LOCUS       DAWABZ010000001        37390 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_324_length_37390_cov_3.604098, whole genome shotgun sequence.
ACCESSION   DAWABZ010000001 DAWABZ010000000
VERSION     DAWABZ010000001.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 37390)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 37390)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..37390
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_324_length_37390_cov_3.604098"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7978..7987
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         8162 a        10578 c        10226 g         8414 t
ORIGIN      
        1 cctctacgcc tgctacaaac tctgtacgat aaactatatt acgcccagat ggatttcatg
       61 agctctttgt acgattcgcc gaggtggcgg atgatgacgt cgaacatggc gtaaatcaaa
      121 tcgccatcaa cttcatcgtc tttgagaagg acgcacgtat cgaggatgag gttgccttca
      181 tcatcgacat agtatttgaa ggacttgtac tgcttgttgt acgtattgag cagcttgagg
      241 acggcggctt cattgtcatc gtgcaggacc ttcggcgcta ccaggacgcg gatcatgccg
      301 taaatgctgg tgtcgaggat gacgatgagg gacaggcggt tgccattgat gtcagcagcc
      361 gaccggaaga cgaccgtctg gaactggtca tccttgattt catcgaccgt aaaagccttg
      421 atatcctttt cgtccagata tttcttgaaa ttttccgctt ttttattcat gggaaacact
      481 ctcctttttc tacaaataac acttcggttt tattataccc catttcatca gggcttgtca
      541 tataaaaaag cgctgtcgcg tgaagacagc gcctttttga gtattatgtt tgcagtttgt
      601 agttaagttt tttaaattta aagcttatac tgtacccata aggttggaca gtcccctttt
      661 taagtggtta gcagctagtg gttcgtgatt agcagatggt tttatagttt gtaatacgga
      721 tcttgtagag gcgccacgtg agcggcccgt tagggattgg gataattctt ccagcaaacg
      781 gggcggtaaa tcatacatat cccgaagcgg gcgccccacg tggcgcccct acagaaaagg
      841 gactgccaca tgtgcgacag tcccttttct tattaggaga ggagtagttt ttcgaggatt
      901 gggtctaatt cttccggtgt cgtgctgggt tcaaagcgct ggacctggct gccgtcccgg
      961 ttgatgagaa atttggtgaa attccatttg atgccgttgc cctgggcatt gtccggatag
     1021 tattcggaaa cgaccttctg gatgagctgg ccgctttctt tatcggtttc gtagccttta
     1081 aaaggcgcgg ctcccgtgag gtattgataa agcggagctg cctgatcacc gcgaacgtcg
     1141 attttcgcaa agagcgggaa ggtgacgcca tggttgaggc ggcagaactg ttcgatgtgc
     1201 tggttatctt ccgggtcctg ttccaggaac tggttgcacg ggaaaccgag gatgaccagg
     1261 cctttatcct tatatttctg atataaggct tccaggccgt cgtactgtgg tgtaaagccg
     1321 catttgctgg ccgtattgac gatgagcagg actttatctt tatattggct cagggataca
     1381 tcgtgaccct ggatatcttt gacagtgaaa tcgtatacgt tcatgacgtg acctctttat
     1441 ggtttataat tcgtggataa cgcagccgcc aaattccggc gagcaggtgg ccccgtcgag
     1501 ggacggcgct tctttgacag cgaacgactc cttccacagg ccgtggaggt tgcagtatgc
     1561 atagacttcg ccggcatcgc ctgtttcaaa ttcagccttc ggttcttcac ctggtttgag
     1621 gtaacggatg tcgacgccgt tgtcatcgac aagggcaatc cattcgatgt aatgcttttc
     1681 ttccatcggg tgtgtcactg agccgacgtg gacttccaga tgaccatcga ttttctggat
     1741 agccggtacg tgtttttctt tagctgcatc gacagtgtcg gcgacgagat gagtcaattc
     1801 ctggccggca tagatgagtt tttccggtac ttgcgtgcct acagatacta aaacgctgcc
     1861 atctttatcg ttacggtaaa attctacgtg tttcatatgg gttcctcctt cggggcttgg
     1921 ccccttaata attatttttc tttatgtgct cattatagac taaatcggta cgcttgtcaa
     1981 taatggagaa taattattct ttattatttt ttcttgatat ccagctcaat gctgtgcggg
     2041 tctttcttta gggccttcat ccgttcccgc gtcgtccggc tggcaccgcc ccatttgagg
     2101 accagtccac cgtttacact ccacgtcgac gtcatattgg agccggaccg ccttgtcaat
     2161 tcgccccaga cggaattccc cttccccaaa gacagggacg tcccaaaagt catgtcatac
     2221 caggtgtaat ggctggaacc gacatcggcc gtctggggtg cctggccatc gccatggaga
     2281 ctgcccgtcg tagtcccatt catttcccgc tgaacgccaa agcggccata gagcaggccg
     2341 gcatggccgt tgacgctata cgatttgccg gccataatgc ccaggcgtcc ggtgacatag
     2401 ggtttattcc cggaccgata ggtcacgtcg tctccgctga ccgtgtaagg gaagacataa
     2461 ctcagggaca ggccggcctg gggttcccaa aagaattgct tgggcaagag acgcgtttca
     2521 taatagcgta taccagcagc gatcagccag ttccggtagt tgaccaaatc gctattccca
     2581 tcggcatcgc tataagagac agtatgctgc aactgggaca ggcggatgac ggcatcaccg
     2641 tgacggtcgt gcttatcgcc ccattccatg gcaaaaccgc cgccatattc cttgatagag
     2701 tcagacccgc caccataggg ctggcttcct gcctgaccat tagtatatac attcgacgtg
     2761 ctcatcgtat aaaaagcgcc ctgatgcagg gtcccgcccc aacgggccat ggcatgcggc
     2821 gaatcataac cgatgagggt cgcactgtag tcctggctga aagacaggct gccgtaatcg
     2881 ggaaaggtat agctgcgata atcggtcttg acccaccagc ccgtatcttc tgatggttcg
     2941 gccaagtctt tataagaagc gaaacgatta tcatcgctgc gccgccagaa aaggtccgac
     3001 gccatttcat aaaaagaagc cgtcccagcc tgtgtcgctg ccaaagaccg gccggcagac
     3061 gaccagtccg ccgttcctgc agcagctgct ccggccggac agaccaggag cgaaaaagcg
     3121 attatccaat tacgcaaacc aggcagactg aatttcataa aaacctcctt cgaggactta
     3181 tgtttccgtc cttactaaat cataatggcg gcagccctgt tcttcacggc agcacaggac
     3241 accgtggtcg acgagatatt cgatgtgggc cagcgtttcc cccagggcga accatttctg
     3301 cgtcggcgga aagtccttcc actgcttgcc gcgcatggac caggtcatat gtggggcgat
     3361 gtcgtaggcc gtgctgtgcg gatagagccc tgccagatgg cagatttccg ccaggcgtcg
     3421 gtcgtgatgg gccacgattt cgtcgatgcg gccggcaatg gacgtatcgc cttcacggtg
     3481 tcccggcaga gccagtcgga cggggagttc ccgcactttc tgtaaacttt cgagatagcg
     3541 ttgcagggcc tgtttcatat gataccagac ctggatgttc ggcgtaatgt cgaagaggat
     3601 gtggtcactg gtaaagaaga cttcttcttt cgccaggtac aggcagcaca ggcccggcgt
     3661 atggcccggc gtgtgaatga cctgtaaatc gcagccggcc aggtggaggc agtcgccgtc
     3721 atggacaggg ttcaccggga aatgcgggtc tggcgaaaag gtccgggcct gattggagaa
     3781 ctgcgtcttg atttcggccg gcggcatgcc ttcgcgcatg aagttatctt cagccgcttt
     3841 ccacgtcgaa ccgtcttcgg aatcgacgag gtactggtaa tcgacgggat gcatgtaaat
     3901 aggacagccg gcctgggcaa agaggccgac gagaccgctg tggtcagcgt ggagatgagt
     3961 gatgaacagg gacgtatcag attgtaaacg gatgtccagt tcttgtaaac cctggcacaa
     4021 ggcgcgttta cattcaggac ggttaaagcc cgtgtcgatg actagattgc cagacactcc
     4081 tttgatgaca taggcgttga gatactgcag gggattgtca ggcaaatcga cgcggatacg
     4141 gtatatatcg ggatgagaat gaacttgttg gatcatggga cctcctttta aaggaatcag
     4201 gcgggccgcc ctctgggacg gccctacgaa taaaggcctt taaatttaaa accattcact
     4261 aaaaactaca gactgcaaac ttactactca aaaagaggct gtcagatgac agcctctttt
     4321 ctcatttctt ctttttagga ttatagcggt tcatgccttt gtcgacgccc aggctcaggc
     4381 agccttcgat agctgtcgct gcgtcttcaa tgccctgctg gtaggcttca cggagttctt
     4441 ccggaaaagg agtcaggacg tggtcgacga cgtcatggtg cggcaagggc cggccgatgc
     4501 cgacgcggaa acgggtgaaa tcggtacagc cgttagcaaa caggctcttg atgccgttat
     4561 gtccgccatc gctgccgctc ttgcggatgc gcagacggcc gacggccaag tccatatcgt
     4621 cataggcgac gtagacatcg ggctgatcga ttttatacca gcgcatgagc gggccgacgg
     4681 cgcggccgct ttcattcatg tacgtctgcg gcttgaccag gatgatcttt tcgccgttca
     4741 ccgtggtctg ggccacgagg gcgtccatct tatcctgcca caaggtcacg ttccagcgct
     4801 tggctaattc atcgacgaca cggaaacccg tgttgtgttt cgattcttca tattggcggc
     4861 ccggattgcc caggcctacg atcatatgca cgacaggtcc cttatttgtc gaagagacgg
     4921 cttacggaaa cgtcgtggaa gatgcgcatg atagcttcgc cgaggagcgg tgcgaccgaa
     4981 aggactttga tcttatcgat ctgtttttcc tgcggcaaag gaatggtatt ggtgatgatc
     5041 agttcggaaa tgactgattt ctggatgcgt tcgacagccg ggtcggtgag gacagcgtga
     5101 gcgcaggcag cgaagacacg ggaagcaccc atttctttca gggctttcgc gccaccgcac
     5161 aaggaaccag ccgtatcaac gatatcgtcg acgaggatgc aggtcttgcc tttgatgtcg
     5221 ccgatgatgt tcatgacttc agcaacgccc ggacggggac ggcgtttttc gatgatggcg
     5281 ataggtgcat tgacgcggtc agccagatcg cgggcacgcg ttacgccacc gaggtcagga
     5341 gatacgacga cgatgtcgcc catatccgtt tcttcttttt tctgattgat gtatttggca
     5401 atgatggaag ccgagccgag gtgatcgaca gggatgtcga agaagccctg gatctggccg
     5461 gcatgcaagt ccatggtaac gacgcgggta atgccggctg tcgtcatcaa atcagcaacc
     5521 agtttggatg taatcggttc acggccgcgg gtcttgcggt cctgacgtgc ataaccataa
     5581 tacggaacaa cggcggtaat gtgtttagcc gaagcgcgtt tgagggcatc tgccatgatg
     5641 agcagttcca tcagggtgtc attggccggc tgacaagtcg gctggatgac gaatacgtcc
     5701 ttaccgcgga cactttcgtc gatcatgacc tggatttcac cgttgttgaa tttgccaaca
     5761 aaggctttgc ccagttccaa accgaggtaa tcggcaattt ccctggccaa ttccggattg
     5821 gcattccccg taaaaatctt cagctttttc ccgtcttcgt aactcatagt gagcctccct
     5881 gtataagtag tgattaattg ttatttttta tatgtatctt ccttgaccca gccttcaatg
     5941 ttgcgctgcc gtgcccgggc aacggataag gctttggccg gaatatcttt ggtaatcgtc
     6001 gaaccggcgc cgacataggc atagtcgccg actttgaccg gtgcgacgag gttgctgttg
     6061 cagcctacga aggcatggtc gccaatgatc gtgcggtgtt tgatcttgcc gtcgtaattg
     6121 acagtgatcg tcccgcagcc gatattgaca ccactgccga catcgctgtc gccgatgtag
     6181 ctgaggtgcg gcagtttcgt gccatcgccg acggtggagt tcttgacttc catatagttg
     6241 ccgactttga cctgattgcc gatgaccgta ttgggccgga aatggacgaa cgggccgatt
     6301 tcacagccgt tcttgatttc gcagtcatga gcatacatga actggacatg gtcgtcatcg
     6361 cccatcttga cgttagtcat gcggacgtac gggccgacct ggcagccttc gccgatgacc
     6421 gtatcgcctt cgaggatcgt ccccggataa aggacggtgt cgcggccgac ggtgaccgtc
     6481 gtgtctacat aggtgttatt cggatcgatg atggaaacgc catcggtcat gagttcatcc
     6541 agtttacgct ggcgcaggac ccgttcggct tcggccatct gctgacgcga gtttacaccg
     6601 agtgtttccg cataggacgg cgcagcaaag gcactgacga ctttgcctgc cgatacgagc
     6661 atgccgatga tatcggtcag atagtattca ccttgcgcgt tgtcattggt gacgttgtga
     6721 agcatatccc acaagagaga catatcaaag cagtaaatgc cggcgtttac ttcgtcgact
     6781 gccagttctt ccggcgtgcc gtctttctgt tcgacgattt tgacgacctg ccctttttca
     6841 tcgcggatga cgcggccgta accggtcgga tccggcatat gtgccgtcag gaccgtcgct
     6901 gcagcgccgc tctgttcatg gcagtcgata agggctgtaa acgtttcctt cgtgaccagc
     6961 ggcgtatcgc cacaggttac gaggagggtg ccagcttccc cctggagcag cggttctgcc
     7021 tgcaggacag catggcctgt gccgagctgt tctttctgca tgacagtttc ggcctgatca
     7081 ccgaggtaat cccggaccgt ttcaccgcca aacccgacga cgacgacctg gcgctgcgtc
     7141 ccggctgctt tgacagcccg cagtacctgt tcgaccatcg ggacgccgcc gactttgtgc
     7201 aaaaccttgg ggaacttcga cttcatacgc gtccccttgc cggcagcgag gataagtgca
     7261 atagtatttt tcatccagtt atagcctccc tgaattccat aactatataa tgtccattta
     7321 aggataacac aaaacggggc gaaaaaaaag aggctgtcac ataaagacgc ctcttttatc
     7381 aaacaagctc gtattaacct aatacataga caacgacatc acgacggccg aagtcgaggg
     7441 cttcgctggt cgaatccatg gccaggtcga tgcggtcacc gacgatagct ccgcccgtat
     7501 cggcagctac ggcatagcca tagccttcaa tgtagagctg cgagccgagg ggaatgacat
     7561 cggggtcgac ggcaaccatg ccgcgctgga gatactgtcc cgttgccgtg tagccatcac
     7621 cgccgccatc ttcagcggtg taagccgtag cttccatgac aagacgacgc tgatagcctt
     7681 ccgtcgaaga cgattcttca gggatcggac ggccactggc ttccgacagg gcttccatgg
     7741 tctgggagcc gacgacgccg tctgccgtga ggccgtggtc agcctggaac tgttcgacag
     7801 cagcttctgt cgagggacca aaatcaccat cagcactacc ggacagatag cccgtatcct
     7861 gaagcatata ttgaacgctc tgtacgacag agccactcat acccatttga acgctagcac
     7921 aagccaggga tgtaaacgcg aacatgcaga ttgttgtcat taatatcagt atacgatnnn
     7981 nnnnnnnttg aacgaacaca atatcaagta cgtcctgaat tataacaaac ttcccactat
     8041 aagtcaaaaa aattaataat tataatctgt ttttgatgca tttattcttt attctgtaca
     8101 ccgtttaaac agcgtatgtc cgttaatgct caacttcgag atgtattttt tagtacctgg
     8161 tactagtttt ttataattat tatgcatttt tacctatttt agctcttcgt cttctccaca
     8221 atatttctta tggattcagc cccttctatg gaatcggtga ggacgtaaat gaagtctcct
     8281 gcccgcagac acgtatcgtc atcagggatg atatctccac tgccgcgctt gatgtcgacg
     8341 atgaccgtat gggccggcca ggcaatgcgg ccgacatatt tcccatcggc ctgactgccg
     8401 ctggatacgg tcagttccag gagattacgc cgttcttccg acgataagac gggcttattc
     8461 ttggccaggt tccgctgcaa taaagcttca taaatcggtt cgccctggca gagctgagct
     8521 acgaccagtg cgaccaatga cgccgtacag aggacaagca ggtgctcata agagctggtc
     8581 atttccatga tgagaatggt tcccgtcacc ggtgactgaa cagaggccgc aaaaaaggcg
     8641 gccatggaga tgacgacgat gttggacaag taatacgaag aaatgagtcc caagcggaca
     8701 aagacgatgc ccgtaatgcc gcctgtcagc gcgcccagga ccagcatggg caggaaaaag
     8761 ccgccaggga cgccgcagcc atagctgacc agggtgaaga ggaacttccc gatgagaagg
     8821 ccggcaaaga gtttcagcga taagggcagg gtatagaggc tgttgaccag gtcgttgccg
     8881 ccgcccagga cttccgggaa gacatagccc aagatgccgg ccatgcacaa ggcaaaggca
     8941 atgcgcatat aattattgcg aaagacaggt aaatcataga aataatgaat attcagcaaa
     9001 cccttgttga agacgacgcc ggccaagcca ataatgattc ccagaatgac cacgagccac
     9061 atgtagcgca ggggaaacgg cggcagcatg ccgaaatgga agaccggcgc ccggccgaag
     9121 acgtagcggc tgaccatggt cgccagcagg gccgctgcca tggacggcgc caggacgacg
     9181 ccggaaaaat tgcggtgcag ttcttccagg gcgaacatga cgccggccaa gggcgcatta
     9241 aaggccgccg ccagaccggc actggcaccg gccgtgatga ggtagcgttc ttccatgcgc
     9301 gtccgcccca aggcccggct caggccctga gccgtcacgg cgccgatctg gatggacggc
     9361 ccttcgcggc ccagggacag gcccaggccg atgccgacga tgccaccgat gagcttgacc
     9421 cagagaatgc gcagccagcg catgcggatg gcccccaaga tgacgccttt tacctggggg
     9481 atgccgctgc cgccggcatt gggttcataa atccccaggc accataagag cagggccgcc
     9541 gccaataaga cgccgaacca ggacaggttc aggagtacgc cgccgctggc taggacgttg
     9601 gcatagatcc attggcgcca gaccgtcccc atttccaggc cccagcgaaa aaagctgata
     9661 accgttcctg ataatatccc gacgacgatg ccttcaaaaa aaaggcgcag gcggaaccga
     9721 ggccaatggt cgagggcccg cagagccgct tctaactgtg tatcgacatg catatctttc
     9781 acaatccttt ctattcatat gtccaaatta tatcatagtc ccgtaacttt cgcaggttcc
     9841 tgatggtatc ccctattata gtattataga gaaggcgaaa tataagtttt cgtaaatttt
     9901 tcgcaagcat ttcctaaaaa tcgttgctat ttaaagaatg actcattatg atagaaacaa
     9961 cacatcgata ggagtcctgt ccatgaatca gccatctttc catttcccaa aaagcccgcg
    10021 ggccaaggcc atcgcctttg gcctcatctt tctcctgact tgtatagcca tttatctcta
    10081 tagccattcc agtgccgtcc caaaagtaaa aggcacggcc cagaacgtgt cggtcacggc
    10141 gtataccgtg acccgccagg atatgaagcg caagatttcc ctgtccggcc agaccgttcc
    10201 cgtcgcccag gtcgacattt ccacgaaata cgccggtaaa atcgccgatg tcgccgtcaa
    10261 cctcggcgat gtcgtcgcgc cgggccaggt cctgctcatc caggatacgg tcgacacgaa
    10321 cctgaccctc aatcaggata aagcagccct ggaacaggcc gtcgccgatt ccaaggctgc
    10381 agaatcgcaa ttcggctcag acctgcaaaa agcagaagtc gaatacgaca cggccaagat
    10441 gaattacaac cgctatgccg tcctgaaaaa cgaaggcgcc atttcccagc aggaactgga
    10501 tacagcctat caggccctga tcgtcgccaa atcggccctg gacaacctgg agtcgcagaa
    10561 tgtcggcgac gtcccggcca gtgtggccag taaatatgcc gcccaggcca aagccggcta
    10621 taccgtcgac agcctgaccc agcagctcga cgacatgacc ctgcgggccc cgcgggccgg
    10681 cgtcgtcacc taccgcaacg ccgaagtcgg cgccatggcc gcggccaaca cgaaagtcat
    10741 gaccattacc gatacgagcg gcgtatacat cgactgttcc ctgtccgaag ccgacgtcgc
    10801 cgccatccag caggggatgc ccgtcagcgt atccatcgag tccctggccc aggactacga
    10861 cggctatatt acctacgtca gcccggccat ggacgatacg accaagacct acgtcgtccg
    10921 catcaccctg gcccagccgg acgcgaagct gcgcggcggc atgttcgccc agtcgtccat
    10981 cgacgtcctg cagaaaccgg ataccctgtt catccccaaa gacgccttga cggaacagga
    11041 tgggaaggct caggtctgcg tcatcaggcc tgacgatacg attgaactcc gcacggtcac
    11101 gacgggcctg cgcaacgacg attatatcga aatcacggac ggcctcgatg acggtgacgt
    11161 catcgccaca accaacttgg cccgcctcaa agacggtacg accgtatcca tcgaagaaag
    11221 gcagtgaccg tcatgaattt gactaagttt tctatcgacc gccctatcgg catcactatg
    11281 gtcatcgcct tcttcgtcgt cctcggcctg ttcagtttct accgcatcgg cgttgaactg
    11341 ctgccggcct tgaacacacc gtacgtcacc gtgtccattt cctaccccgg tgccaatgcc
    11401 gattccgtcg aacagcaggt cgtcaagcct gccgaagacg ctctgtcttc tgtgtctaat
    11461 ttaaaacata tgacgtcgac ggcatcgtac ggccaggccc ggattacgct ggagcttgat
    11521 ttttcggcca atgccgatgc ggcagccatt gacgctacga aaaaaatcaa tgccatccgc
    11581 ggcagcctgc ctgacgatat cgacgagcca gtcgtcatca agcgcgacgt caacgcgacg
    11641 ccgatcatgt acgtcgccgt ccaatcttcc cactccctgg acgatatcta cagcaagaca
    11701 gaaaatgatt tccaagatgt cctccagcag gccgatggcg tctcggaaat cgagctcaac
    11761 ggcggccgtg ataaggaaat cgccgtcgaa atcgacaagg acaagctcat ccactacgat
    11821 atgaccctgg cagaagtcgt caaagccttg aaagcggaaa acgtcctcat gccgtcgggg
    11881 acgatttatt cgtcaggcac gacgacggac gtccgcgtca aagcccagta cgccgatgaa
    11941 agcgaactgt cccaaatcca ggtcaccaat gccaagggtg ccaagattcc cattacggcg
    12001 gtcgcctcca tcaagcgcca ggaccagcga gtcacgcgtt atgcccgtgt caacggcgac
    12061 gatgctgtcg tcatggccat ttacaagaac agcgacgcca acgtcgtcaa cacggtcgac
    12121 aatgtgacca aaaagctgga cgaactgcgc caggacaatc cggactatac ctttaccatt
    12181 accaatgaat cggcttcgta tatccgcaat tccctgcaca ataccctgca gaccctcatc
    12241 gaaggcctgt gcacgacagg gctggtcctg ttcttcttcc tccggggctg gcgatcgacg
    12301 ctggccgtca tgattgccat tccgacatcc ctgatttcta ctttttttgc catgtacctg
    12361 gccggcttca cctttaacat gatgtccctc atgggcatgg ccctgtgcat cggcatcctc
    12421 gtcgacgact cgattgtcgt attggaaaac atccaccgcc acctcatgca gggcgaagca
    12481 ccaaaatcag cagcccttca cggccggtcg gaaattggca tggctgctgt cgccatcacc
    12541 ctctgcgatg tcgtcgtttt cctgcctatt gccttcatga cgggcatggt cggccagtat
    12601 ttcaaacagt tcggcctgac catcgtcttt gccacactct tttccctctt cgtttccttt
    12661 acgctgacgc cgatgctggc atcgcgcctg ttccgcctgg gcctgaaaat cccggacaaa
    12721 ccgatttggc ataaaatgga cgccatcgaa gaaagcgccc gcaaccacta tacgtctatc
    12781 ctgacctgga gcctggatca cgccaaaaat ctggtcatcc ccgtcctggt cctgtttatc
    12841 gccgcagcgg ccctgatccc gacgggcctg gtcggttcgg aattcatgcc ccagactgac
    12901 gaaagcagtt tccgcatcac cgtcgaactg ccgaccaatg ccaatctgac gcggacggat
    12961 aaagtagcgc gccagctcga agattacttg aagacggtcc cggaagtcac gtactatacg
    13021 tcgatgatcg gcggcaagag cctgaaccag gccaatatcc aggtaacgct gaaagaccgg
    13081 cgcgaccggt cgcggacgat atggcaagtc accaacgacg tgcgccgctt tgctgcggac
    13141 cacatctatg acggcgacgt ccgggtcaaa gaagcccagg cttccgtatc cgggacgaca
    13201 ggcggcttcg gcggcggttc aggcaacctt cgcctggaac tgcgcggcaa ggacagcgag
    13261 gccctgctgg cccagtcacg ggctatcgaa cagatgatga agaccgacat cgccggcctg
    13321 cgcgacatca gcagctccta ccaggacggc atgcccgaat accagctcgt cgtcgaccgt
    13381 aataagctga aacaatacgg cacgtccctg agcgatctga acgacacctt ttccaatgcc
    13441 atcagcggcc agaaagccgg cgtcctggcc aatgacgcca aaaacgacaa caacgacacg
    13501 gatatctacg tccgcctcaa aggggccgac gccttccgcc tgtccgactt ggaaacgatt
    13561 cccctcaacg ccaacggcca catcatttac atcgccgacg tagccaaggt ccaggccggg
    13621 acaggaccgg tgacgatccg ccggaccgac aaggaacgga gcatcaccct cggcggctcg
    13681 cctcagggac ggccgctgaa cgaggtcatc caggacgtgg cgaatcacct gaacgccatg
    13741 gacctgcacg gcatgacttt ccgcttcacc ggccaggccg accagatgac gacgaccttc
    13801 acggacttgg gcgaagccct gctcctgtcg ctcatcctga tattcatgat cctggccatc
    13861 ctctatgaat cggtcaagac gccattcatc cgcatgttct cgctgcccct gggcctcatc
    13921 ggctccctgt tcctgctctt cttgaccaat aatacgctga acctctattc ccttatcggc
    13981 atcctggtca tggatggcct ggtcgccaag aatgggacgc tcctcctgga ctatacgctg
    14041 accctcatgc acgaacaggg cctggacccg cgcagcgccg tcatccaggc cggccgcgtc
    14101 cggctgcgtc ctatcttcat gaccaccctg accatggtcg tcggcatgct gcctacggcc
    14161 ctgtccatga cggccggggc ggaaacgcgg gccagtatgg ccgtcgtcgt catcggcggc
    14221 ctgctgacat cgacagtctt cacactggtc attatcccga ttcttttcgt cttcatggaa
    14281 aagcacagcc tgttccgacg aaagagtgcc taaaaaaagt cctgccctgg tggcaggatt
    14341 ttttgtttta ttatagaata atatatacat atatagaaaa tgcatcgggc tcaatgaagg
    14401 aggactgacc tatgaacgat agatacacac cgacatttaa tattcacgaa gccgtgcgca
    14461 agactttacc ttatatgatg gaaatgcgcg aatatttcca tgcccatccg gaattgagtg
    14521 aaaaagaatt cgatacgtgc aagacgatat tccaggaact gtccctcatg ggcatcgaag
    14581 acgttaaaat catcgccggc acaggcgtca ccggcctgat ccacggcgcc ctgcccggtc
    14641 cagtcatcct cttgcgggcc gatatcgacg ccctgccgct gacggaaaaa ttcgactgcc
    14701 cttattcgtc cctcgtcgac ggcgtcatgc acgcctgcgg ccatgacggc catgcagcca
    14761 atctcctggg cgtcgccaaa atcctcaaca agcacaaaga ctgcctgcgc ggcacgatcc
    14821 gcctggcctt ccagccggca gaagaaggca acggcggcgc cgcccgcatg atcgacgccg
    14881 gcatcctcaa caacccccgc gtcgactatg ccctgggcct gcacgtcgcc ggcagcctgc
    14941 ccaaggggta cgtcggcatc ggccgcggtg agatttcggc gtcctgtgac gacttcacca
    15001 tcaccctcca cggccgcggc ggccacggct cccagccgtc cctgtgcatc agccccatcc
    15061 agatgggcat cgacatcatc cagtccatcc agaacttcgt ctacaactgc catgccgacg
    15121 gcgacggcgt cgtcctcacc ttcggccagt tccaggccgg ccacaacccg aacgtcatcc
    15181 cggaaacggc cgaactgaaa gggaccctgc ggacatataa cgaagaaaag cggaaacgca
    15241 tcctcgacgc cattcaggcc atcctgaaaa atgtcactac cacttacggc ggcaccttcg
    15301 acctggacgt cgtccccttc tcgccagtct gcgtcaatga cgacggcgtc accgaccggg
    15361 tcgtccacat cctccaggac cactgttccc accgcgtcga cctggtcccc ttgaagcgcg
    15421 gctccggctc ggaagacttc gcttatttca gcgccgccgt gcccagctcg ttctactacg
    15481 tcggcgtcta cagcgacgca ccggtcatcg gccattcacc ggaattccat tgggacagca
    15541 acgccttgaa gtacatgtgc gaaacgatgc tcaccatcgt ctattacttt gaaagttatg
    15601 cctgagcttc ccagttgtct aattccatat tgagcgtata atgaggggta tccatcttga
    15661 aaaaggagtg atacccctca tggtattctg gttcatcatc gaacacacga tgcttcccat
    15721 tttctttctc gtcgtcctcg gttatttcct cgataagaaa ttccacctcg acgtaaagac
    15781 cttatcgaag ctcatgtttt tcctggtcat ccccagtttc atctttacca atatctatac
    15841 gacgcaattc ccggcgacga gcatcaacat catcgcggcc atcgccatct tcatggtcat
    15901 ctgcttcgtc atcgccaaca tcgtcggccg tatccgccac tacgatgcag ccatgctgga
    15961 atcgtgccgc aatgccttca tgttcaacaa cacgggaaat ctcggcgtcg ccctgatcat
    16021 cctcgtcttt tcccacgacc ccttcgtcgt caacggccag acgccgtatc tggccgaagc
    16081 tatggttgta cagatcatca tcttcatgat ccagagcatc tccctcaaca cgctcggcct
    16141 ctatcaggcc ggccggggcc gcctgacggc cagggatacg ctatctgtta tgttccacat
    16201 gcccctgctc tatgtcctgg ctgcggccct catcgcccgc tacgtcggct gggacgccaa
    16261 ggacttcttc ctgtggccta tcatggatat ggccagcaag gccctcctgc ccctggccat
    16321 ggtcagcatc ggtgcccagt tgtcgcagac gaagatccaa tggctcgaca aggacgtctg
    16381 gattgtcagc atcctcaaga tgacggtcct gcccctcatc ggcctggcca tgatctacct
    16441 ggccaatgcc ttccggcccg gcacgttcac agccgtcagt gccacggtct tcctcatcta
    16501 ctgtgccatc ccgacggccg tcaatacggc cctgtttgcc atcgagttcg acaacaaccc
    16561 cgattacacc acgcaggtcg tcatgaacac gacggccctg agcgccgtat ccctgacttt
    16621 ctatattttc ctggggcaca ttcttttcgt ctgaatgata tactttttat ctgttcgtta
    16681 ctaaaatcaa tatatcccaa cttttattaa aagaatttca aaaaaagtta ttgacatgac
    16741 gcgtttttac ttatataatg atttcatcaa cagaatagca gcggcaaagg agcctggaaa
    16801 gagagagcat aaactatctt tccaggcttt tcgtatattc tggcgataat aaaatatgac
    16861 agtagacaat gatgtctttt ccccgatggt tccatcagga aaggcatctt ttttatttgt
    16921 caggaggttg ggaattatga aaaaattatg gtcacgttta ggagtgctgc tcccgtgtct
    16981 gctcatggca gcgccggcag ccttcgcttc cgatccgggg acgatcgata caggcgatac
    17041 ggcctgggtc ctcatcagcg cagccctggt attcatcatg acaccgggcc ttggcttctt
    17101 ctacggcggc atggtccgca acaagaacgt cttgacgacg attatgcaga gcttcttcat
    17161 cgtcgccatg atttccgtag aatggatcct catcggctat gccatgactt tcggtacgga
    17221 tattaatggt ttcatcggtt ctcttgataa agtcggtctt accggcgtcg gcatggctgt
    17281 cctggaaaac gggacgattc cggaaatggc tttcgtcgcc ttccagtgca tgttcgccgt
    17341 catcacaccg gccctgatta ccggcgcttt cgccgaacgc atgaagttcc acgctttcgt
    17401 cgtcttcatc ctgctctggg ctatcttcat ctacaacccc atggcacact gggtctgggg
    17461 cggcggcttc ctggctaaac tcggtgccct cgacttcgcc ggcggcctgg tcatccacat
    17521 cctctccggt gtttcgggcc tgaccctctg cgtcctcctg ggcaaacgct atggctatgg
    17581 gaaaatcccc atggttccgc ataacctgcc tatgaccgtc ctcggcgcag ccctcctctg
    17641 gttcggctgg ttcggtttca acgccggcag tgctctcggc gccaacgcac tggctgccag
    17701 cgcgttcatc gcttctcagg ccgctgccgc tgccgctacg gtcacctggg tcgctgtcga
    17761 atggattgtc cagggtaaac cgaccatcct cggtgccgct tcgggctgca tcgccggcct
    17821 ggttgccatc acaccggcag ccggcttcgt cgctcccctg ccggccttga ttatcggcgc
    17881 tgtcggcggt gtcatctgct acttcgccgt cgccgtcgtc aaagccaaac tcggttacga
    17941 cgattccctc gacgccttcg gtatccacgg tatcggcggt acatggggct ccatcgccac
    18001 cggcctctgg gctacgacgg aagtcaaccc ggccggggct gacggcctct tctacggcag
    18061 ctcggatctg ctcgtcgctc agattatttc cactgtcgtc gcttacgccc tggccattgt
    18121 cggcacgttc atcatcttca agatcatcag cctcttcatg acggtccgca ccgataagaa
    18181 tgaagaaatc acaggcctgg acattggcga acacggcgaa cggggttata attatgctat
    18241 catgtccggc agccccttcg gcgaaacgcc ggcgacgccg ctcggcaatg catccttgga
    18301 caatgccgcc gtcaacaacg caggcattaa cggtcgtaac ttatagaggt gatacgaatg
    18361 gaagaacatc cgattacaaa agtagaaatc atcactcgtc cgagtaaatt ggaagagctc
    18421 aaagaagccc tgaacaacat cggtgtcatg ggcatgactg tcagccaggt cttcggctgc
    18481 ggcctccaga aaggctacgt cgaagtctac cgcggccagc ggtacaacat caacctcctg
    18541 ccaggcatta aagtcgaaac ggtcgtctgt gaagtgccgg tcgacaaagt cgtcgaagtc
    18601 gcccagaaag tctgcaagac aggcaaaccg ggcgacggca agatcttcat ctaccgcctg
    18661 aacgatgccg tccgcatccg gacaggcgaa cgaggaccta tcgccatcat ggacgaaaag
    18721 tgagtgtaca aacggggctg ccgcatcgcg acagctcctt tcaagttgtt agtggctagt
    18781 tgttagttgt tagcggatgg gtttaaattt aaaggtcttt tctacaaact ataaagcaca
    18841 aggtgtttcg catgatgata taaatcttcg tatgacggcc ccgctttttg ttattagggg
    18901 ctaacaactt atcatttttg cgctataata gcgtcatgaa agaaaaatcc actgcaacta
    18961 ctcacaagag gaggaattca acgtatggtc aaaatcggca atgttgcctt cgacaatggc
    19021 atgcctaaaa tctgtatccc ccttgtcggt cattctacga acgaactcgt ccaggaatgc
    19081 cgctatctcc aggataaaat ctacgacgtc gtcgaactgc gcatcgactt cttgaaagac
    19141 gtcacgtccc tcgacgctgt cggcgaagct ttggccgctg tccgccagga attgccaaac
    19201 ggcgctatcc tgttcacgtt ccgtactaaa gaagaaggcg gcgaaacgga agttcctgaa
    19261 agctactatt tcgacctcat cggctacgct attaaaagcg gtaaagtcga tgccgtcgac
    19321 gtcgaatact tccgcgaccg cgccagcatc gaaaaagcac tggtcgtcgc gaaagaacac
    19381 ggcgtaacgg tcatcatgag caaccacgac ttcgacaaga cgccgtcttt cgatgaaatc
    19441 actggccgcc tcatgggcat gaagaaactc ggcgccgatg tggccaaact ggcctgcatg
    19501 ccgaactcgg ctaaagacgt cctgaccctc ttgtcggcaa cagaagccgt caaatcccag
    19561 tatcccgatg aaccgatcat caccatgtcc atgggcaaac tcggcgccat cagccgcatc
    19621 agcggggaaa tcttcggctc tgccctgacc ttcggttcgg ctaaaaaagc ctctgcaccg
    19681 ggccagctgg aagtcacgac attgcaacaa atcctgcggg cccttcactg aggccttcat
    19741 atatttctga taagtgcttc aagggcatag agtacttctt ccatgctttt atcattgaca
    19801 atgcaataat cagccgcttc acggtaaagt gtgatgcgtt cttcgtagag ccggtagact
    19861 ttctgccggc cctcttttaa taaggggcgg acagctacat ctacatcgga gacgatatca
    19921 tctggtgaac ggtcgatgaa aaatactttg cccgtctttt ttaaaatagc catattttcc
    19981 ggacgtttga tgacgccgcc gccacaggca atgataacgc cctgcttggc agccagcgta
    20041 cgactggctc ggacttcggc atcgcggaag cagtcttcac tgacggtaaa gagttccggg
    20101 atggtcttac cttcctgttc tacgatgacg tcgtcggcgt cgtagaaatc acgtcctaag
    20161 cgtttcgcca aagctttccc taaagtactc ttaccgcatc caggcatgcc gatgaggaca
    20221 atattgacct tatcattcat aataagattc catctccttg gcaatttgtt caatcacaga
    20281 cgaatcgata gtccggccca tccagatttc ttccgaaccg atggcctgag cgacgagcat
    20341 gtacatgccg ttcagggtct tggcgccgtg gcgacgggcg gcctgcaaaa tctgcgtgtc
    20401 tttcgggtta tagacgatat cgacgacagc cggaaaggcg gcgatgatac gttccgatgc
    20461 agggcagccg ttgacgtcag ggaacatgcc gactggcgtg cagttgacca ggacgtcgcc
    20521 gccctgagaa tattcaaagt cctgataatc gatagtatcg gcaccgacgg tcttcgtgaa
    20581 ggcgtcgaat tccgtcttcg agtgaggatg gcgggatacg acggtaatcg acttggcacc
    20641 gctgtcggcc aggtactgga tgatggcccg ggcaccgccg ccggaaccga ggacgacgca
    20701 gtccttcttt tggatgccga taccggcatg ttccagggag cgaccgaagc cgctgtagtc
    20761 tgtattatag ccaaaaatcc cgttatctgt aaaatggagc gtgttgacag ctccgatgat
    20821 gcgggcttcg cgggaaatat ccgtcaagta cggcatgacg tcgagtttgt agggaatggt
    20881 gacattggct cccgtatacc cttcgtcgcg caggcgctga agttcatttg ggatatcgct
    20941 gcggtccatt tccaggacgc cgtactgatg ctgcccttcg gcctgtaaaa tcctgaataa
    21001 tttttcgtgg atagccgggg aagccgtatg acctaactta gccccgatga cgcctaattt
    21061 catctacatt tcctccttgg tcggatacgt aattccctaa gattttaaaa taattgcagt
    21121 gttctgccaa attgcgcagg acccgggatg tcgccggatt gcgcaggttg cccatgacgt
    21181 cgatgtggaa aaagtattca aagggccggc ccttgagggg ccgcgattcc agatgagtca
    21241 tgttcatgcc gttatagaag aagtagccca ggacgtgata caaggcgcct ggccggtgct
    21301 gtgtcgtcag gactagggta atcttgtcgg cctctggcga ctgttccata tcggcggcga
    21361 tgatgaagaa ccgcgtataa ttcatggtat tgtcattaat atgttccacc aggacatcga
    21421 ggccgtacat atcagctgcc gtcttgttgg caatggcagc gatatggggg tcctccattt
    21481 tttgaacctg ttcggcactc tgagaggtac tgaaataggg gtggagctgc cattcgccgt
    21541 ggtgcttgag gtaactgcgg cactggttca ggccctgcgg atgcgaatag acttcgcgga
    21601 tgtcttcgag tttcgtccct ggcagggtca agaggcaatg ctctactttg acgtattttt
    21661 cgccgacgac gcagcagtcg taacggtgga tgaggtcgta tacatcggtg atgccgccag
    21721 tcgacgaatt ctcgatgggc aggacgccgt agcgggcctg ccgggtagca acggcctgaa
    21781 cgacgtcttc aaagtgagag ccataagcag gcaaaatatt cttgccggca aaataggact
    21841 tcatggcttc atacgtataa gaaccgggct ggccgaaaca ggcgatgttc aagggcatat
    21901 cttcagacgc atccattacc agcaccgccc tttctccgac aggcagacgt cgaggatagc
    21961 ccaggcagcg gccgcttcga cgacgggaat ggcgcgctgg acgatgcagg gatcgtggcg
    22021 gcccttgatg acgaggtcgc agttttcctg cgtgtccgtg tcgatggttt cctgtttctg
    22081 gctgattgac gccgtcggct tgacggccag gcggaagacg acaggcatgc cgttggtgat
    22141 gccgccgttg atgccgccgt tgtggttggt ccgggtcttg acgacatcgc catcgtaata
    22201 aaaggcgtcg ttggcctggg agccgcggag gtcagccagg gcgaagccgt cgccgaattc
    22261 gatgcccttg acggccggta cggagaacat catgtgactg agctggcttt cgacagagtc
    22321 gaagaacggg tcgccgatgc cggcaggaag gcctgtcacc atacattcga tgacgccgcc
    22381 gacggaatcg aggtcgttct tggcctccag gattttttct tccatcgggc cggcctgggc
    22441 cttatcgagg acgggcagtg tttccttttt cagcgcctgc agggtttctg ctgtttcccc
    22501 ggcagcatca aagggcttgt cggcgatgcc ggccaggcgc aggatatggg agccgatggt
    22561 aatgccgtgg cgggccagta tctgcgaagc gacggcaccg gcaaagacca ggggcgccgt
    22621 cagacggcct gagaagtggc cgccgccgcg gtaatcgttg aaaccgccat agcgtatctt
    22681 gccggaataa tcggcatggc caggccgcat gatgtggcgc agctggtcgt aatcccgcga
    22741 gtgctggtca ctgttgcgga tgatagcgca cagcggcgtc cccgtcgtat ggccgttgaa
    22801 aaagccgctt tgtacttcaa aggcatcttt ttccgagcgg gccgtagaca ttttattctt
    22861 ccccggcgcg cggcgggcca tttcagcccg gacggcgtcc cagtcgatgg cctcgcccga
    22921 cggcaggccg tcgaggacgg cgccgataga aaggccgtga gattcaccga aaatcgtaag
    22981 gccgatcgtt cttccaaagg tattcatttt actccccctc cttttggatg atgccgccta
    23041 aggcctggaa atcctgccag aattccggat acgatttgcg gacacactca gcgccttcga
    23101 tgcgcagcgg ctggctgcag cgctgggatg cgacggccag ggccatggcg atgcgatggt
    23161 cgttccagcc gttgaccgta ccaccggtaa agccgccgac accgtggatg atgaggcctt
    23221 cgtcttcttc ttcgatatcg ccgccgattt tgttgagctc tgtggccatg gcgtgcagac
    23281 ggtcacattc tttcaagcgg acccggccgg catggatgat gcgggtaatg cccttggcac
    23341 aggacgccag gacggtcagg accgggacca ggtccgggca gtcttcgacg tcgatgactc
    23401 gtccagtcag ttctgacggg caggccgtca attccgtctt gccttcgacc tggtcgccca
    23461 tgtcgcggat gagagagacg atgacttcgt cgccctgaga cgatttcgac cgcagacccg
    23521 tgcagcagat agcccggccc aggctgccag ccgagagcca gaaagcggcc tgggaataat
    23581 caccttcgac gacataagtc ccggatcgat agtgctgatt gccaggaatc cgataggagc
    23641 cgtcccattc tttatcgatg acgatgccgt gctggcgcag gcagtcgagg gtcatgtcga
    23701 cgtagctgcc cgattccagc ggtgtcgtac tgcgcaggac cgagtcgccg tcgagaaggg
    23761 gcaaggtcat gagcaggccc gtgaagaatt gagagctgac gtcgccggcc agggaatacg
    23821 tcccgggctg caaggtcccc gatacggtca ggggcagggc gccttccgtc gtttcataag
    23881 tgatgccctt ttcatcaaag agttcataat acggcgtcaa cggccgtttg gccaggcggc
    23941 ctttgccgga aaaagtaacc ttattgccgc tgaggatggc gacgggaatg aggaagcgca
    24001 aggtcgatcc cgattcattg gcatcgacgg ccagcggctg gccctgaggc ttcaagccgc
    24061 cttggacgcg ataggcagcc cggccggaat gagctgaaac ggcaggtgtg atgtcggccc
    24121 ctaaagcctt caggatatca cacgtcgctt cgatgtcctg ggatagggag atgttgtcga
    24181 ccaggctttc cccttcggcc agggccgcgc agatgaggtc gcggtggccg atacttttag
    24241 aagaaggcgc ttctaccgtc ccttgtaagg tatgtggtgt aatgatgaca ttcattgggc
    24301 gtccttcttt cggatgtatt tcggcaggtc ttccgcagcc agcggcagca ggcgggcctt
    24361 gccgatttct tcgataatga ccagggtaat atggttgccg cgcatcttct tatcattgct
    24421 gatgtaaggg atgagttctt cagccgatgc atcgacatcg atcggcaggc catactgttc
    24481 gaggaccttg cgcagccgcg ccgtcgtccc ggctttcgtc agccccatgg cttcggtgac
    24541 accggtcagc atggacatac cggcagcgac gccttcaccg tgggcgtagt ggccaaaacc
    24601 ataacagcct tcgatagcat ggccgatagt atgaccaaag ttcaacatca tacgttcgcc
    24661 catatcaaag acatcgtgtt caacgatatt ggccttgctc tggcaacagc gttcgatgat
    24721 ttcttcccaa tgctcgaaaa tagactggtc atcgagcttt tccaggagtt caaagagggc
    24781 caggtcgcgg atgcagccgt atttgatgac ttcccccata ccgtcgtgga tgaagcgctt
    24841 gggcagcgtc gatagcagtg ccggatcgat gaggacgcct ttgggctggt aaaaattgcc
    24901 ggcctgattc ttgccgctgg gcaggtcgat ggcaactttt cctccaacgc tgctgtcgac
    24961 ttgggaaata atcgtcgtcg gaatctggat aaagggaacg ccgcgcaaga aggtcgctgc
    25021 cgcgaagccg ccgaggtcgc cggtaacgcc gccaccgaaa gtcaggatac aatcgctgcg
    25081 ggtaatgcca gcatcagaga gctgctgata gaggtctagg agggtcgctg cgcatttgtg
    25141 ttcttccccg gccggaaaga ccaggcggtg aacgcggaag ccagcctttg ttagaaccgt
    25201 ttccaatcgc tgaccgtaga gggcatcgac gttcgaatca ctaatgacag ccaggttctg
    25261 cgatttcgtc aggttgcgga tataatcgcc agcccggtcg agaaggccat tttcaatatg
    25321 gatatcataa gagtcttttc ctaaatcaac gtgaatgtgt ttcatgaact aattcccttc
    25381 tccgcatagc ggcttgctgt aaaaaacggg tcatctcttg ggcatcttct tcggccagca
    25441 tggtcgagaa agattgcagg gctgcgctga agcggttgat ttcatacaag aggttgtctt
    25501 tattggataa gaacagcttg gtccacagcg gcgcattgat gtcggcaacc cgcgtcccat
    25561 cgcggaaact gccggctaca aaatacttgg tcagcgtatt catggattcg ctgttgacca
    25621 gagccgtcgc caggacgtgg ggcaggctgc tggtataagc gatgaagcgg tcgtggtctt
    25681 ccggcgtcac gcggacgacg tgggaacagc ccagggccat ggccatatcc ttcacagccc
    25741 ggatatggtc ttcgtcgttg cccgccatgg ggacgaggat ataattggca ccgttgaaga
    25801 tatcggcact ggccatggca tacccccggc cttcacggcc tgccatgggg tgaccgggca
    25861 cgaaatcaac gccttccggg agaagagcgg caatagccgt cgccgtatcc cctttgatac
    25921 cggctacgtc cgtcaagacc gcccccggtg caaagaaggc tttattttct gccaggaatc
    25981 gtatcatcac atcagcggcc gtacaaaaga tgaccagatc cgcctgcttc aatgccttac
    26041 tgccagccgt atagatttca tcgaccatgc cttcctgctt agcctggtgg agggcttttt
    26101 catcgggatc gatggcgatg atatgtccga cgccgatgcg ccgcaggccg cgggcatagg
    26161 aaccgccgat gaggcccagg ccgacgatgg ccacagtctg tttcgtatta aaaccactag
    26221 cttccttatc catgattgtc tccccctgta tcccttatat ttcccggccg acgacgggtg
    26281 ccagtttgcg gagcgaatcc atgaggtcgg cgaacatggc cggcgtaatc gactgagcgc
    26341 catcgcacca agccctgtcc gggtcgttgt ggacttcgat catcaggccg tcagccccgg
    26401 cagcaacggc ggctttggct aggtcggcta ccatccagcg cttacccgtc gcatggctcg
    26461 ggtcgacgac gatgggcagg tggctctggc gtttgacggc caggacggca ctcaagtcga
    26521 gggtgttgcg ggtgtacgtt tcaaacgtcc ggattccccg ttcgcagagg atgacgttgt
    26581 ggttgccttc ggccatgatg tattcagccg acatgagcca ttcttcgatg gtcgcgctca
    26641 ggccccgctt gagcaggacc ggcttgcgcg tcttaccgac ggctttgagg aggtcgaagt
    26701 tctgcatgtt ccgggccccg atctggatga tgtcgacgcc atcttcgagg aagaccggca
    26761 gtttgtcggc cgacatgatt tccgtaatga ccggcagttt cgccttgcgg ccggcgtcgc
    26821 ggagcatgtc gaggcccttg ttgcctaaac cctggaagga gtacggcgaa gtacgcggct
    26881 tgaaagcacc gccgcgaagg atgttggcac cggccttggc gacgtcgtag gcgacgcctt
    26941 cgatctggtc cggcgtttca atggagcagg gaccggcgat gacggccagt ttcttgccgc
    27001 caatggtcac gccgctgtta ccgacgggaa tgaccgaatc ctggggatgg aacgcccggc
    27061 tggcccgttt gtacgggact tcgacgcgca tgaccttgtc gacacattca tagctctgta
    27121 tctgttcgac atcgacgact tccgtattgc cgacgaggcc catgacagtc tggcgggcgc
    27181 ctttactgat atcgacctgc aggcccttgg ccttgatggc atcgactaat tttttcgctt
    27241 cttctttcgg tgtgtttgct ttcaatacaa tgatcataac tttttcctcc attccagcaa
    27301 caaaaaaagc aggctcacat gagcctgctt ctaagattct cgtttatcgc cttttcctcg
    27361 cgtcttgctt aggcagccat agccggctgg cacggaaaag gtggaagtct tctcatgctt
    27421 acatcggttt tattgaattc gtcgtagcaa aaataaccag cgctaaaata caggtagccc
    27481 cagctaaaat aagtaaagac gtattcaatt acctgatgta agaatgacgt gttcatagga
    27541 atctccttgt gcaacattta aaaattcgtt gtcgtcattg tataccccgg ggaaataatt
    27601 gtcaaggtaa aaaagcaggg attctctgta tagatatgag aatccccgtg gacaatcgtt
    27661 ttactgacgg atatattgta tactattttc gcatatcatc tatgtctttt accaagaagg
    27721 cggaatggct gtcgtgacga cgcggaacgt atagccctgc cctttcagga gatggatgat
    27781 gtccggcagg gcgtcgacgg tcgcaccgtg gccataggca tcgtgcatga ggatgaccag
    27841 atggcgatgg agatagggcc gcaattgcag ctgctgcttt atgttttcca cttctttatc
    27901 ggctgtcttg cccgtcccgt cagcatcgcc ggtcaaggca ttccagccga cttcgatata
    27961 gccgatgttg ttgatagccg tccagaagtc cttggtgaag tgcccttccg taccgccagg
    28021 ggcgcgagaa atgatgggcc ggacgccgag aatcttataa atttcttctt ctgtctgcaa
    28081 gagctcgtcc gtataagcct tgggcgaaac gtatattttc ttataatcat gcgtatacga
    28141 atggacgccg atggcattgc cagactggta gattttcttg accacatcag ggtagcggac
    28201 gacattctgg ccggtcagga agaaggtagc atggatgttt tcctttttca ggatatccag
    28261 aatgcgcgac gtattcttat cgttcgggcc gtcgtcaaag gtcagataga cgacgttgtc
    28321 accataataa taatcccgcg tcttgccgta tgtcgtgggc aggccctttt cctgacgctg
    28381 ccgggtcgta tatttatcgt acaccgtttt atcattatcg acaggaggca gggagaaatc
    28441 atcaccgatc agagctacgt caggcttgct ggaaaggacc tgcggcaccg ggatatgccg
    28501 ggcttcggcc gctgccatga cgcggtcgcg gtcgaaattc aggaaaagcc ccagcgaagc
    28561 caggaagaaa agtaagatac atcccaaaaa cagtctgcct ttttgtttca tttttgctta
    28621 catccgtttc tttttgacat taccatagtg aatatactgg cgtatcgtac acgtttcccg
    28681 atagccatcg gccaggacgc tggccgtcag gtcgaagtct tccaaatcgt cgaggagggc
    28741 ttcggtcatg cggtgctgga cggcattata gcccgtgcag aagaggatcc gcgggcagcg
    28801 cacgccgcgg aacagtcccc gcggatgctt gatgagcttg gccagcatgg ccggcgtaat
    28861 gacagcgccg caaggacggc ccatgatggc cgctgccgtt tcgacgcctt cgattttttc
    28921 ggcgctgatg acgttgccca gcatctgtaa attgaggaca ccgatgacgg tatcgcagga
    28981 agccggcagg ggtacatcgt ctgccagcgg ggccttgagc cacttattgc cggctgcatc
    29041 gccttcgatg aggatgtacc aggccgggtg gcgcatcttg atgtcatcga tgaccttggg
    29101 gtcgaggccg atatagcggt cctggtcgtc gaggccggcg aaccacgccg ggacgtggcc
    29161 ggcggcaatg cgttcagcgc agaaggcttc gcccttttcc atatcttccg tgcagatgac
    29221 gtcaaagggt tcgacattat cgacacgcgt gctgtccatg ggaacgatgc tgctgaccat
    29281 gatcggcaga tgaccgccgt ggccgtaagt ccccagccgt tccaatacgg tcgtcttacc
    29341 accggcaccg acaatagccg taatgccggg tttcaacata cgtcgccaac gattatcttc
    29401 aaacatgaaa gtaccccctt ctggaattca cttcttcaca acataacgat tatagccttt
    29461 ccaggccgat ttgatgaggc cgcggaccat atcgatggtc agggcccgcc gttcttcttc
    29521 ggagcaggtg ccgaaattgc cgaaatcgag ttcttcactg gccagatggg ccacattttc
    29581 tttgacggcc atgccctttt ccgtcagttc ttcactggcc gagcgggcaa tggcagccag
    29641 gccgggacgg ccgccgacga cgatgcacca cgaatcttcc cgcatggcga cggcaccgac
    29701 cagatacggt tcatctgccg gcagcttgcg ataggcttta taggccgtat agacagcttc
    29761 tttggcaatg gaaatgcccg ttaccagttc acccatgggc gggcagtgca aatagtcatc
    29821 caggctcatg cagcctttat cctgcagctg tacatcgacg tggagggaca ggaggatcgg
    29881 caggaggacg gaaaaaggcg ctttccccga aatgacgccg cccagcgtgc cgtagcgctg
    29941 gacgttttta tcggctacgt cactgatggc cttgctgatg gctccgccgg caaactcctg
    30001 gatgaccggg gaaatcttga tctgccaaag cggtgtcatg gcgccgatga cgatgtcgtc
    30061 ttcccgttct tcgatatact gcatcccggc tttcgacaaa tcgacagcca ggtcataatg
    30121 gtctttttta tatttcaagc cctgcgttcc cgccaggaaa acagccgacg cagcctgccg
    30181 ataagcagcg taggctgcgg ccggattatc cgggaccagg gtcgtcgtac tggtaatcac
    30241 gatactgaaa atctcctttc tgcctagtta agggatgtcg tcgaagaacc agtcttccag
    30301 ggccacgttc ggcctgtcct tgttgagcag gaaagacccg ccacattcat cgacgacagc
    30361 atcgacaagg gcgtcgacgc cgatgtcgct gcacaaatcc atttcttctt tatataaaag
    30421 gatgggcatg agaagttcca ccgtcgtacc atcggccatg gaaacgccgc ccagctgacc
    30481 ttcaaactgg acgatgacca ggccggacag agccgtatcc ctggcaaaag gctcatggct
    30541 ttccgagttg ggcaggagaa agccatagga catccacaca tcatggacgt gcgggaaccg
    30601 ccccagctcc atgagcagcc agatgggcca ggcttcttcg tcgtttaggg aaatatccca
    30661 gtccttgcgg aagggccaat cagccggcag gatgaggcag agttcgccgt agccttcttt
    30721 cccggtcaca aattcgccag ccgggtaatg catgggcgcg gcactcatgc cgatggtatg
    30781 aaggagatag aagggttctt ctaccgtagg atataagagc tctacgtcca ctgccagcgg
    30841 cgtcccgtcc gggtcctgga agacactcca ctgccgatga ggaaagaggc tgtggaaata
    30901 agcctggacg gcgcggccat attcctcgtg aggccgctgt atcttcaagg tgtccatcaa
    30961 ggaaaaaaag cgaggccatc gataggacgt aatcggtaag gtactcatcg aatcccccct
    31021 tttcgttata ttcagcttca ttataacata aatgagcccg ctctgggcaa ccaaaaaggg
    31081 gcgccgcgaa cgcgaagccc ctctccacta tcatgacgat agacttttta gccctcaggg
    31141 ttcgtgccgg agggattcac tgaatctcat cgcctgttca attgacggga tatatcatat
    31201 caataaatag ggaaattgtc aaatcacagc gccttcggcg cgccttgtaa aaggcaggac
    31261 ggtatagtag aataaaggag aatggaacgc actatattaa ccgcaaaggg atgactacat
    31321 tgaaggtacg ctgggtagaa ttaaaccgtt ttgaagctga actgatcctc gaattcgcct
    31381 gccagcccaa ggatgctccc gtttctgcca gcctgtgccg tatgatatac cgccgtctcg
    31441 aaggccagcc ggcactggca cagaaccagc cgggactcca ggacggggag gcgtaaaagg
    31501 cagcctcact cttccatcgc tttttctaat cgagcggcga tggccttccc cgtttcggtc
    31561 cgggccaggc cgccatcgga ggtttctttc agggcgactg gcatgaggcg gccgatttcc
    31621 gccatggccg caatgacttc atctggcggg atgacgctct tgatgccggc cagggccatt
    31681 tcagcagccg ccagggcttc aacggcaaag acggcattgc gcttgacaca gggcacttcg
    31741 accaggcccg ctacgggatc gcaggccagg cccagcgtgt ttttcatgca cagggcaaag
    31801 gcctggagga tcgtatcgtt atcgccgccg gccatttcgg ctacggccgc tgccgccata
    31861 gccgcagccg tcccacattc ggcctggcag ccgccgacgg caccggcgac agaggcattg
    31921 cgggaaacga cggcgccgat acccgctgcc gtgaacaaag catcgaccag tacgggacgg
    31981 ggcgtctgca tttcttcggc agcggccatc atgacggccg gcacgatgcc gcaggaaccg
    32041 gctgtcgggc aggcgacgat tttgaacatc ttggcattgg cttcactgac ggccagggca
    32101 tagacagccg ccttatgcgc caaggggccc agtaaattcg gcgccttttc agccagttta
    32161 tgggcatcgc cgccgacgag gccgctgacc gattttcccg ggtccttcat gccggcatcg
    32221 atggattcct ggaacacatc gagacggctg gccatgatgg cccgtacttt ttcttccagt
    32281 tcttctgacg actctacttc atgacgcacg acgatgtctg acagagtcgt cttttctttt
    32341 tctgccaggg ccaataagtc atgaagggtc ttatattcat atttatacat cttattcctc
    32401 ctagagcggc agcagcgtga cgacgctttc aatggctgta atattctgta tcatgtggac
    32461 gatttcattg ggaacagggc tgtccgtgca gacgatcatg acggctgttt cgttcttggc
    32521 actgcggaag acgcgcatat tcgatacatt gatatgaccg atggccagga ccgtcgtcgc
    32581 ctgggcgatg atgcccggct ggtccctgtg gaccgtaatc agtgtatgtt cttccccatt
    32641 cagggccact tctgccccgt tgatttcgcg gatttcgatt ttaccgccac ccagggagac
    32701 gccgaggact tccatcttcc ggccagacgc gccggtcatg gcgatgcgta ccgtgttggg
    32761 atggtccact tcttcatcgg accggtggaa ttcaatagcc aggccctggt ctgcggcaat
    32821 ggcaaaggac gtgcgcaggc ggacatcgtc ggcagaaaag ccgagcaggc cggcgacgag
    32881 ggcccggtcc gtaccgtgac ccttataggt cttggcaaaa gaaccataga cagtgatgac
    32941 cgtctttttc ggttcatctt tcaggatttc ccgggccatg aggccaatcc gggcggcacc
    33001 cgccgtgtgg gaactggacg gcccgatcat gacagggccg accacatcaa aaactcccat
    33061 gagaaaacct ccttacagtt tttatagtct gatttcttgt tgtagatgta agctataaat
    33121 gtacgtttct tcgacgggcc ggtgcgtaat ctgttcgaca gcccgcttgt ataatgccag
    33181 ctggacggca tagcgtctgc ggaaggcgtc tgccgtttcc agccggtccg acttataatc
    33241 gacgaggatc caggcatcat cttcgcggaa aaggcagtcg atgacccctt ggatcaaaat
    33301 ctgttcgccc gtttctgtct caggcagagg atcgccgcca gctaacagga cagtgaaggg
    33361 atattcgcga tgcagttccg gcgattcggc catgcgccgg cctatatcgg actgacagaa
    33421 agctgccagg accggcacat agaccagctt ggcctcttcc cggctcaaca ggccctggcg
    33481 gacaaaggcg tcgatttcac gacgcaaagt cgccgtcgtc tgatcggcag ccggcgtgat
    33541 atactgcaag gccttgtgca gggccgtccc gcgctgagct ccgctgacag ccgcttcttc
    33601 tccggccagc cagggcggca gggcggcaaa ggcatcgtcc tcaccgggcg ccggcgggat
    33661 gacggccgca gccgggacag acagggcggc ttcgtcttgc agttcatcgc tgtgcagttc
    33721 ctgatatcgc cgcttgactt cggatacgga aaacttggcc gctgtccgga cagcctgggg
    33781 ataggcatac tgccaggata actgttcgtc cagccaggac gggacaggcg tgccggtcaa
    33841 gtcgccgcac cgcagggctt ccagccgcgg ttccccatca taggcgcctt cttcaacagt
    33901 ccctgccggg acggccttgg cgatgcgcac ctgccagacc gcatcctgcc aggccgcacc
    33961 gggacgggca taatcagcat cggccttggc cccgaagggg gccagggcag ccggcatgac
    34021 ccagtcgaaa tacgatttgg cctgagccgg attgaggcgg ctcgtccagc gctgccagtc
    34081 acggtcgatg tggctgctgt ggcccgtcag gataagctgg tccctggcgc gggtcatggc
    34141 gacatagagg atgcgctctt cttcggccgt cccttcccag cgcagctggg cggcgatacc
    34201 gctccagatg agcgtcggat aggacatgcg ccattccggg tcatactgct tgagaccgat
    34261 gcccaggcga ttgtgaaaga gtatctggtc ctgcgtgtcg cggcggttga aggccttgcc
    34321 catatcggcg acgaagacga cggggaattc caggcccttg ctcttatgga tactcatgat
    34381 gcgcacgacg tcttcctttt cactgacgac tttagccggc gccaggtcca ggccgtcttc
    34441 tttcatctta tccatgagct gcaggtagcg gaagaggccg cggaagccgg cttcttcgta
    34501 ctgccgggcc cggtcgtaca gggctttcag attggcctgg cgcacgtcgc cgccaggcat
    34561 ggccccgacg aagtcgacat aagccgtatc atcatacagg cgctgcaaga gttctgccac
    34621 gccgtggcgg cggctgtacg tgcgccacga atcaaaggcg gccatgaact gctgcaggtc
    34681 ctctttttca tctacgccgt cgggcagcga agcaacaaag gccggcaggt tctgccacag
    34741 cgtcccgtca ccggccaggc ggacaccggc cagggccgtc tcatccaggc cgaccaaagg
    34801 cgaccgcagg acagccgcca tggccaggtc ctgttcggga ttatcgatac agcgcaggag
    34861 ggccagcatg acctggactt cgacggcggc aaaataaccg ccactctgtt cggcatatga
    34921 cgggatgcct ccttcctgca gggcctggat gaggacgtcg gccttgccgg ccatggagcg
    34981 caggaggacg acgatatggc gataagacaa gggctccagc gtgccgtctt tgcgggctgc
    35041 catgcggccg ctggccagga gctcgccgat gcggccggca atgaagcggc attccttttc
    35101 aaaggccgtg ctgccgtcgt cgtcttcctc gtcactcagc gtcggcacga tgtcgacttc
    35161 gacaggcccg ccgatccagc gttcatcgtc cggagcctgc cggccggcgt agagtttttc
    35221 ccgttcacca taatccatgc cggccgcttc tgccgtcatg gcccgcgaga agacagcgtt
    35281 cacggcgtcg aggacgaccg gaacgctgcg gaaattccgc cccaggtcga tgcagcgctg
    35341 cacggctttt tcatcgcggc tgaaagactg gtatttctcc aggaacagcg tcgggtcggc
    35401 caggcggaag cgatagatgc tctgcttgat gtcgccgacc atgaaacggt tgtcctcacc
    35461 ggaaacgagg cgggtaatga gttcctggac gccgttcgta tcctggtatt cgtcgatgaa
    35521 gacctcttca tactggctgc gcagctcttc ggctgccgcc gacggcacgg gatgttccgg
    35581 cgatgcatcg ggggcgagga ggatctgcag gcagaaatgt tccaagtcgc tgaaatcgat
    35641 ccagcccttt tccttcttcg ccgccccata agctttggca aaatccaggg tcagcgtcac
    35701 caggccatcc atgacgggtt tcatggcccg catgccgtcg agccattctt ccggcgtcgc
    35761 cgagaaatag acggactgca aggtcttgat cacgtctttc ttgacatcgt cgcgcacttt
    35821 cttgcaccgt tcccagacgg ctttatcgtc atccgataat ttccgcagtg acttcaggcg
    35881 cgggaagtcg atggcccgga caaagcggct caaatcattc caggaccggg cctgggcggc
    35941 ctgccggaga gctgcctgtt cagccacgaa ggtatcgcag gccggcgcaa aggcttcgcg
    36001 ctggcgcagg tgatagagca cgccgtcata gaggtgggca tcttcttcaa ggatacggcg
    36061 gacggcatcc ttgatggggc cggcccagac catatcgtcg atgacggcgt ccggcgccac
    36121 gtcataagct cgggccgcct ctttcagcca atgttccggc caggccaggc tgcgggcata
    36181 ggtatacatg cggcttactg tatccatgag gatgtcatcg ccgcggtcgc tgccaaacat
    36241 atcggccagc ggatagagga tggacgcttt ctcatcgtct tcatagtaag acaagaagac
    36301 gtcttccagg acctggcggc gcaagaggtt caattcttct tcaccggcaa tggaaaaagc
    36361 cggatccagg tcgatggtat agaaatactt acggatgaca tgctgacaaa aagcatggag
    36421 cgtcgaaatc tgggccgatg gcaataaggc cagctggcgt tcgacgcggg gatccttcgt
    36481 ttccgacaag gccttcatca gggccgtccc gatgcggtcg cgcatttcgg ccgcggcggc
    36541 cttggtaaag gtgacgacca gtaattccgt aatatcaatg ggatattcct tgtccaggag
    36601 gcggcggatg atgcgttcca ccaggacagc cgtcttcccg gaaccggccg ccgccgacag
    36661 gagcagcgtc tgcccccggc tgtcgatggc tgcctgctgc tcattcgtcc aggccatgtt
    36721 cgtcatctcc ttcatcaaac atcttttgaa tcatctcgtc tgtatcttcc cgtcccgccg
    36781 cgtcatacgt attgccggca acggcgacat cgaaccggca gaccggccgg taagggcaat
    36841 agctgcacgg cgcctgctga cccatgagga tgggatggat ggaaatatca ccgtcgccga
    36901 tatggccggc aatatcgcgt atctgccggg ccgcccagtc gagcaggtgg ctccagcctg
    36961 cttcattata gagcgtgtgg gacgaatcgc tcagggtccc gtcttttttc agccgcaagt
    37021 tgaggaaatc ggaaaagccc tgcatcgacg tatccagctg ctgcatgacc tggccgtcgt
    37081 cgaggtagaa accggtcagc ttccctttgg catcgtagag tttcttcttg tcttcctcac
    37141 tgacgcggta ttccagggat accttatcat tgcggacgta acagtatagg acggcagccg
    37201 gaatggcatc gccgccaata ttcagcaaag ccacgaacat atacgtcaaa agctgcagtt
    37261 ccaggcccgt gaaaatctgg ctgatgtcga gctgtttccg gccggactta tagtcgatga
    37321 tgacgacgta tttatggtcc ccctggcgca aagtatcgat gcggtcgatc tggccggtga
    37381 cgacgacttg
//
LOCUS       DAWABZ010000002        34363 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_364_length_34363_cov_3.405008, whole genome shotgun sequence.
ACCESSION   DAWABZ010000002 DAWABZ010000000
VERSION     DAWABZ010000002.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 34363)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 34363)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..34363
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_364_length_34363_cov_3.405008"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    23935..23944
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7287 a         9100 c        10059 g         7907 t
ORIGIN      
        1 cggttaccct ctcagtcagc tgcgctgaca gctctcctaa caggagagct gggccgccct
       61 ttgggcggcc cctacgaacc acgagccacg aatctcccag gaggtgattt tatgtattat
      121 ttcgataatg cggcgacgac ggcgcagaag cccgaggccg tcgcccgggc cgtttacaat
      181 gtcctgtcat cgggaaccgt gggcaacccg tcccgcgggg cccatggcta cgccctgaag
      241 gcctatggta tcgtcttgca ggccaaggac gatgtaaagg ccttgttcca ctgcggcccg
      301 gaatatgatg tcgcctttac gcataactcg acggcagccc tgaacatggt cctcaaaggg
      361 ctcatccatc ccggcgacgg cgttttgacg acgtcgtggg aacacaatgc cgtcctgcgg
      421 ccgctttacc agttggaagc ccaaggcgta tccctggctt tcatcgccag cgacagtccc
      481 ggcggcgcct tgcagtatga caccctggaa gaacggctga caccgcggac gaaatggttc
      541 gtctgcaacc acgcgtcgaa cgtgaccggc aatgttttgg acctgccccg catcaaggcc
      601 ttctgccagc gtcaccatct ggggctcatc gtcgatgtat cccagacagc cggcgccatc
      661 gacgtcgact tatccgatgg catcgtcacg gcagcctgct ttacgggcca taagtcgctt
      721 tacgggccag gcgggacggg cggcatcgtc atccgccgcg atgctctcgt gacgccgtac
      781 atcaccggcg gcgacggcgt ccattccttc gagcgggagc agccgtcggc tgtccccggt
      841 gtctttgaag cgggtacggc caacgtagcg ggcattgccg gcctcgacgc cgggataaag
      901 cagctcctgg atggtggcgt cgccgctgcg gctgctcatc aggaagcact ggcccggatt
      961 ttcgtacccg ccgtccaggc tatcccgggt atccgcctgt atggcgactt ttccggaccc
     1021 cgcgtcggcg tctacagcct caatatcggc gatgcagaat cggccgtcgt cagcgacatc
     1081 ctttggcaga cataccagat ggcgacgcgc ccggcctatc actgtgcgcc cctcatccat
     1141 caggccctgg ggacggccgt ccagggagcg gtacgctttt ctttttccca gttcacgacg
     1201 gctgacgacg tccgggccgc tgtccgggcc ttgcgggcca ttgcggcgat gtaagatttt
     1261 ctggcaatcc atcaggaaaa cttgactgct ttggtatagt aatatataat agatatagtt
     1321 ataaaagaca aatagtcaaa agaaaggtgg aatctctcat ggaatataaa ttcacagccg
     1381 ataattttca gcaggaagta ttgaacagca gccagccggt catggtcgac tttttcgcta
     1441 actggtgcgg cccgtgccag atgatgggac cggtcatcgc caatctggcc gaagaatatg
     1501 ccggcaagat taagatcggc aagatcaatg tcgacgaaaa tccgggcgta gcccaggcct
     1561 atgaagtcgc cacgatcccg aacctggtct tcttcaaaga cgggaaagtg gtcaaccgcg
     1621 tcatcggtgt cgtcccggac aatgtattga aagaaaaatt agacgctttg ctcaaataag
     1681 cagctggcgc cggaggagga ttttcatgaa aaattattcc tatatgaccc aggggacttg
     1741 tgcccgtcag attaccttca gcctagaaga cggcaaactg catgacgtcc atttcgtagg
     1801 cggctgtccc ggcaataccc tggctctcag caagttgttg gaagataccg atgccgcccg
     1861 ggccgtaaag ctcctgaaag ggaatccctg cggtgaccgc ggtacctcct gtgccgatca
     1921 actggccatc gccgtcgaac aggccttgaa acaaggcgcc tgatggcgga gtcagtctat
     1981 ggcgtcgccc tggtgccgtc gacacggcag gctatggaag gcgaaaagct ggccaaggca
     2041 gccggcctgg ccgtgcgcat cattccgacg ccgggaaagc tcgacgcgtc gtgcggcttt
     2101 tccctgcgct atgaactggt tgatgaagcg gccctgacag ctctcctcga tgaggagggg
     2161 ctggcatggg tcgctttata tcatgcctgc cgccagggcc tgaccgtcac gtacactaaa
     2221 atcagagaag ggtgatatta tggccagaca tatcgtcatc gggacggccg gccatgtcga
     2281 tcatggcaag acgacgctgg tccgggcctt gaccgggatc gatatggata cgacagcgga
     2341 agaaaagaaa cggggattga ccatcaacct gggctttgcc tggctcgacc tgcctgacgg
     2401 tacgcgggcc ggcatcgtcg atgtaccggg gcatgaaaag ttcatcaaga atatggtcgc
     2461 cggcctgccg ggcctcaatc tggtcctgct cgtcgtcgat gccaacgaag gggtcatgcc
     2521 gcagacgaaa gaacatctcg acatcttgac ccttcttggc gtacataaat tcatcatcgt
     2581 cgtcaccaag gccgacaccg tcgacgacat gctgctcagc ctgaccctgc aggacatccg
     2641 ccagaatctg gccgatacgg cagccgaagg ggccccggtc gtcgtcaccg atgccgtcag
     2701 cggccgcggc ctgacggaac tggtcgatga aatccaggcc atggcagcga cgctgcccat
     2761 ggaaagcgct caggggatgg gccgtctcaa cgtcgaccgg tcgttctcca tcaagggatt
     2821 cgggactatc gtcacgggga ccctcatcga cggccccatg atgaatggcc gcgaagtctg
     2881 gctttatccc ggcggccggc gcctgcgcat ccgcaatatg caggtccacg agcaggatgt
     2941 cctgcgggcc gacccgccgc agcggacggc attgaacctg gcaggcattt cctgtggcgc
     3001 catccatcgc ggtgacgtcc tctgtacggc gccggatttt acagcgacgc ggatgatcga
     3061 tgtcaaagtg acctgcctgg ctgacgcgca gccccttttt ctatggcagc gcatgcgcct
     3121 cctcgtcggg acgcgggaag tcatggcccg catcgtaccc ttgggagccg aacagattgc
     3181 gccggggacg gagggctttt tgcagctgcg cctggagcag gatgaaatct atgtcaaggc
     3241 tggggaccgt ttcatcctgc ggaccttttc gcccatgcat accgtagccg gcggggaaat
     3301 cctcgacgcc catccgcaga aacaccgccg cttcaaagac gacgtcgtga ccagcctgga
     3361 agcccgcgat gccggtctga cggacgacgt cgtcgccggc ttcctgcgcc tgcgccaggt
     3421 cccctttacc caggctgacg tcattgccgg ggcgacggac ctgcccctgg ataaggtaga
     3481 gctggccctg gaccatttgc ggcaaaatgg gattgtccgc cggacccgtc agggctacat
     3541 ccacggtgac gtctataaga cctggcaggc caaggccctg caagtcctcc tggcctatca
     3601 tagagagaaa ccgctgcagc ctgggattcc ccagccggcc ttccggagcc gcctgcatct
     3661 cgatgaaaat gacgggacgg ccctattgcg gctgctgacg gctggcggca tctgccgcct
     3721 gtcccgccag tgcgtagcgg cgaaacaatt ccgtatcacc ttttctccgt ctcagcggaa
     3781 attgcagtcg gctattgaaa agaagctgga ccacagtggc tatgtgcccg tcccggtccg
     3841 cgacgtccgg gctttgggcc gggaagccca ggccgtcgcc gatgccctca acggcaagag
     3901 cctcgtcttc ttatcgcctg atttcatctt gtcgaagcgc tacatgatga aagccgctga
     3961 agcggcctgc cggtatctgc aatcaggctg tccgctgaca ctcggcgatt ttcgcgatgt
     4021 cctgggagtc agccgccagc aggccctgct catcctggaa tatatggacc gctgtggcat
     4081 tacccgccgc gttgccgacg ggcgcctggc cggaccggca gcccggaaat atgaaataaa
     4141 aggggagaat gaccatggat gaagaacgac agcagctgct ccgtcagctg cccgatgtag
     4201 accatttact gcaacggccg gtactgctga cctggcagga gaggaaatcg cgccaggctg
     4261 tccgcgatgc cgtccggaac gtattggatg aacggcgtca ggccatcttg gccggccagt
     4321 gggaccagcc tttttccggc gatgactttg accgggccgt cgtctccctc ctgcaggcag
     4381 aagaaacggg ccatctgcgc cgggtcatca acgccaccgg gacggtcctg catacgaatc
     4441 tgggccgggc tgttttcagt ccggacctgg cccgtcacat tgccaatctg gtctgccatt
     4501 acagcaattt agaatacgac ctggcttccg gcaagcgggg atcgcgctat gaccatgtcc
     4561 gggaacttct ctgtgagctg accggtgccg aagatgccat cgtcgtcaat aacaatgccg
     4621 ctgccgtcct cttgaccctg tccagcatgg ccgccggccg ggaagtcgtc gtgtcgcggg
     4681 gggaactcgt cgaaatcggc ggctccttcc ggattcccga cgtcatcgaa cagagcggcg
     4741 gccgtatctg cgaagtcggt acgaccaaca agactcattt gtccgactac gaacgggcca
     4801 tcggcgaaga tacggcggcc ctcatgaagg tccacaccag taattaccgc atcatcggtt
     4861 ttaccgagag tgtctccgct ttggacctgg cccgcctggc ccatgaccac ggactgccct
     4921 gcatcaacga tgtcggcagc ggcctgttcg tcgatttggc cgcatgggga ctgcctcatg
     4981 aaccgacggt ccgcgaagtg ttggacgctg gctgcgatgt cgtgactttc agcggcgaca
     5041 agctcctggg cggccctcag gcgggtatca tcgtcggtaa agccgcctat ctggatgcca
     5101 tgaaacacca tcccctgctg cgggccctgc gcgtcgataa gatgaccctg gccggtttgc
     5161 aagggacttt gcagacctat cgcgacggta cggccgtttg ccagatcccg acgctgtact
     5221 gcctggccca gacgaagaaa gagacgcggg aacgggcgga acggctgttg caggccctgc
     5281 atacagatga cctgccggcc tgcgtttccc tgcgccgtgt agacgattgt gtcggcggcg
     5341 gcgcctgccc cgatgccgtc ctgccgggct gggccctggc cattgatccc tatgacctgt
     5401 cggcggaaaa actggaacag cgcctgcgcc aggccgacat ccccatcatc gcccgcgtcc
     5461 agcactgcac cgtcgtcctg tccctgcgca ccatcggcga cgacgaattc ccggacgtcg
     5521 cccgcatcgt gcgggaagcg ttgggcgttt gagcgtcgcg tggaggtttc tgccatcatg
     5581 tatgagcggg ccgttcttta tagtttgtag tacggagttt gtagtttgta gaagagggtt
     5641 ttgaattaaa aaaacttcac tacaaactgc aaacttaata ctcaaaaagg cgctgtcttc
     5701 atatgacagc gcctttttac atttcctacc gcggggatga ttggccgatg atttcttttc
     5761 cttctacgaa ctggaccgta ggattcggtt ttttcttttc aatgaggtcg accatgaggt
     5821 gcagacagtg ctgaccgatt tcgcccatgg ggatatcgac gcgggacagg gagggaatgg
     5881 cgtaatcatt gaattgagcc atgttgatcc cgatggtgaa gacttccacg tcctgtggga
     5941 tagcgatacc cagttcatgg caggtataga gcatgccgtg ggccaggacg tcggattcac
     6001 agaacatagc ccggggcatc cgcttttctt catagagctt cttcgtagct tccacagcgc
     6061 tcgatgaaga cgtatcgcag aggatgatgt tttcttctgg tacggcgtgg ccggccttgg
     6121 cgaaggtgtc gaggaagccc tgcatccacg tgcgcatgat cgggaacgac gtgtcatggg
     6181 tcaggacgcc ggcgtcgaag atgcccttgt ccaggaagtg ctgggcgacg ttgcagccga
     6241 tagtatagtt gtccatggtg acgctgctgt atttatccga ttcccggtta aagaggacga
     6301 cagggcaggg gatatggatg gaattgaggt agtccatatc tttattggac atattcgcga
     6361 tgatgatgcc gttgaaaaat tcattctgca tggcttcgag ctgtttttgc agctggtcga
     6421 ccttataagg gacgacggtc agatgaatgg ctttgtcctg tttcatgatt tcttcctgga
     6481 tgccgagggt gatgcgggcc aggtagttga tacggaagtc cgttgcccag tagaaggcaa
     6541 tggcatagct gtccttttta gccgaccgca gttttttggc cgactggttg gggacgtaat
     6601 tcaggtcttg tgcaattttg tatatacgct gttgtgtttc ctgcgagatt ttccgttccc
     6661 ggcccttgcc gttcaggacg atggaaacgg tggcgatgga aacgccagca gctttggcaa
     6721 tgtctttaat tgtggacacg atggccacct ccactcttgc ttttattttg acataaatta
     6781 aacgtttttt caaggaaatc tgtgttacag gatgaacaaa tattcatttt tatgtaagtc
     6841 gcgaatcgtt tgcaatcatg gtacaataga agcgattaag acgagaaaag aggatgtgta
     6901 tgacagtgag caaagaacgt taccggcgcc aggcccttca tcaaatcgac aatctggcca
     6961 agccgccggg aagcctggga cttttggaaa agcaggccaa gcagatttta ttggcctggg
     7021 ggacctttca ccgggagctg cggccgaaac acatcatctt tgccgccgac aatggtgtcg
     7081 tccgggccgg tgtcgtcgcg cagttgtcgg atatcacgta tatgcagagc tgccatatgg
     7141 tacaggggac gtcggccgtg acctgtttct gccgcagcca ggatatccct tatgaagtcg
     7201 tcgatgtcgg tatcgacagt gatgatgccg tcggactgga ccgcaagatc gccaggggga
     7261 caaagaactt tgccgtcgaa ccggctatga gccgggaaga gctggcgcaa gccatggcga
     7321 cgggccgcga acgggtgcag gcagcggcca aagatggcat caatctcctg tcattcgggg
     7381 aaatgggcat tggcaacacg acgacgtctg ccgcggtcct tacagcattg tcgccgaaaa
     7441 agatgtcctg gctgactggc ttcggttcgg cccggggcaa ttataaactg ctcttccata
     7501 agcgccatgt catcgccgca gccgtcaagg cctatgaaaa agaaatccag atgccggctg
     7561 atgccctgcg ctgcgtcggc ggctttgatt tggcagcact ctgcggcgcc atgatggcct
     7621 gtgccgacga aaagattccc ttttatatcg acggcttcat tacggcgaca gccctggcct
     7681 gtgccatcgc catgaagcct gccgtccgcg attatgccct gccgtcccat atttcccggg
     7741 agcccggcat ggcccaggcc ctgcgcctgt gcggcatcga tgaatacgac gtgcccatcc
     7801 aggccggtct ctccctgggt gaagggacgg gggccgtctt gggcgtcgtc ctcatgaaga
     7861 ccatgatgta tgccgtctgg catatggcta ccctggacgg catcaaccag gaagcccagg
     7921 accgccatga caaccgcaaa ggagatgaaa cgcatggatg aagaaacgag aaaaaaagcc
     7981 caggccatct ttgagcgcct gggactgacg gaagaacaag cagttgaact cttttataaa
     8041 cggaccattg cggccgggaa aatgcccttt gctgcagccg aaggggaaac caagaaaatc
     8101 aagaaaaaac gcatgaacga acgcttcgcc gaatgggatg ctttttagcg gaccgcagaa
     8161 gctcaattcg tgcagccatc tccgcttgtc tttgccgggc tgactgcgtc gaaaagcctc
     8221 gacagagccc cgctatgcct gcgtttttct ccttgccagc ccggcaaatc cggcgcggat
     8281 ctgaccgcgt cattgagctc ctgcggtccg aaaggctctc ctgcgaaggg gagctggccg
     8341 ccgaaggcgg actgaggggt tcttaaatgt acgagccacg aaccatgaac cactttaaat
     8401 tatttttacg aaagaaggga ttttgatgat gtatccgtta ttacatgcaa cgctgaatgt
     8461 tgggtctctg gttttgaaga accgtctggt cctgccgccg atggcgacgg aaaagtcggc
     8521 gaaaggccag gtgacggatg ggctggtcgc ttattatggt gatatggccc gcagcggtcc
     8581 aggcctgatc attcaggaac acagtttcgt ttcgcctgaa ggccgggcca gtgccaacca
     8641 ggtgtcgctg gcggccgatg ccgatattcc cggactgcag cgcctgacgg cggctgtcca
     8701 cgctcagggc gtacccatcc tggcacagat cagccatgcc ggcagtgctg cccatcgggc
     8761 cattacgggg caggaagtca tcagtgccag tgctgtttcc aatccgtcgc gggccgcttc
     8821 ggtccagggc cagccggaat ttccccggga aatgacgcag ggcgatatcc agcgcatcgt
     8881 ccaggccttt gtcgacgccg ctgtccgggc acagcaggcc ggctacgacg gcgtggaaat
     8941 ccactcggcc catggctatc tgctggacca gttctattca ccggtgacca ataagcggac
     9001 cgatgactat acgggccaga ccttggctgg ccggacccgg ctgcacgttg aaatcattca
     9061 ggctatccgc cgccaggtcg ggtctgattt tgtcctttcc ctgcgcctgg gcggcagcga
     9121 ttatatggat ggcggtgccg tcgtcggcga tgtcagcgaa gcggctaaga tttttgaaac
     9181 ggccggcatc gacatgctca gcctttccgg cggcatgagc gtcttcttcc gccctggcca
     9241 tacggaagcg ggatattttg ccgaattatc ggcagcagcc aaaaaaggga cgacccttcc
     9301 cgtcttgctg accggcggca ttgccgacgg cgccgaagcg gaagccttcc tggaaaaagg
     9361 gcaggccgac ctcatcggca tcggccgggc ggcactccgg gaccaccaga tttaccaaaa
     9421 aatcttgtcc atgtagaaaa aaagcgctgt cgcatgaaga cagcgctttt tgagtattaa
     9481 gtttgcagtt tgtagttttt agtgaatggt ttttaaattt aaagtcgtct tctataaact
     9541 acaaacttta aagagaaagg agcctcgtac gatggcagaa gccttcatgt gacagctatg
     9601 aattatgttt taatgaatac ggtctgtttt tttctgagca aaaagccatt ctaaggcggg
     9661 ctgcatggtg taggcatgct gccaggtaga gcggtggctg ccgccttggt actgggtcag
     9721 gtagtggctg cagtccggcg tgatgagggc agcggcctgg gcgtcgacct ggtcttgcgg
     9781 ctgggtgggg tcgagggtct ggctggctac tttgacgccg tctttttcta agttttccat
     9841 gatttttgcc aggctcgggc tggcaccggg gtcaccttgg gaggtaacgg cccagatatg
     9901 ctggtgacct aagggcgaag tgacggtttc gtcccatttg caggcgacga ggtaagagcc
     9961 ggcgaagaag tccggatatt tgacatccat ggcgatggat gtcatgccgc ccatggactg
    10021 ccccgtgtta tagatgcggt ccgtatcgat ggaatattga cggcagagct gttggacgag
    10081 atggatggtc cggtccagtt ccggcccgta ttgatagttg tcatcgacga tgatcgtatc
    10141 gtattgaggg gccaggacga agcaggggtg cttggcctgc cattccggcg aggcgaaggt
    10201 aatccaaagc ctgccatttt ttcaatttca tgagaaaacc tcccttttag actgtcgcac
    10261 gaaggcttct gccctcctgc gaggttcttt ttcttttatg gtttatatta tagtatagca
    10321 tttgtagttt gcagagaaaa cctttaaatt taaaaccatc ccctagcccc taacaactaa
    10381 aaagcgtaaa cgggcgcccc acgtggtcgc ccctacagaa aaaaggcttg tcgcatatgc
    10441 gacaagcctt ttttatttac ttatcatatc gtctgaggcg aagcccgtgc gattggcgta
    10501 gtcgccttcc aggatttgga cggggacgaa gccgttcttg tctttgtcac cggtaatctg
    10561 gatgcgcgtc ggcttggcaa agaccagtat cttgtcggac cggacgaggg agcggattgt
    10621 ttccatatcg cggtcgtggc tggctttgag gaaggtggga atggtcgtct cgtcggggat
    10681 gacgtaggtc ccgcgcatca tgaggtagcg gccgctgttc gagggcaggc gattcttttc
    10741 ctgctgcgct tctttttcct cgtcgctcag ttccgggacg ccgctggctg aatagcggga
    10801 gaagttgaca ttggtctgtt cggcttctcc atagcggtgg acgtaataga tggtgatgaa
    10861 caggcagaag atgacgaagg gcaggaagag catcttcgtg taatacgtca gtttggcgta
    10921 tttggcttcc ttttcttccc gcgcttcccg ttcttcccgc gtctcgacga cttcttccgg
    10981 ctggccctgg agggcaccgg ccaggatggc cgacaagggg ataccgtagc tggaaatggg
    11041 atagaggttt tccagccagt tcaagccgca gatgcaggcc atggcccggc tcagggcatt
    11101 gtccatgtcg acggggatct tcatgcggtg caggatcgac gtcagcgaat cattggccag
    11161 gttgggcatg tacgtcgagg ccaggaattt caagtcccgg atgtagacat ccttcagggg
    11221 cagttcccgc ccggatgctt catagctggc cttgatggcc ggaaagagcc gttccgagcg
    11281 gtaggcagag aggacggcgt ctttgaggag aaaggcaatg tcttcatccc agacgacatc
    11341 gaaaggcttg ctgtcggcca gcatggcttc cgtcaggttg tgtgcctgga ccgggccttc
    11401 ccggggtttg atgaaatagg aaatgccgtg gacattgccg tcgctgatcc aggccagcgc
    11461 caggcggcag atactgttta agttgtcgtt ggccaggtcc gctgcgatgg cgatggacga
    11521 attgaccttt cgccggtgca gcgattccag cgccttttgt gtattcgcat ccatggcggg
    11581 tatcctccgt gaatcgagtg gttttaacgg cctcgctgtt tatagtaggg gcggaacgtg
    11641 cgccgcggcc gcttgggcgt ttgtttgagc ttgaagtagt tgtgcttgac tgtgcagttc
    11701 ttttcgtctg taaaggtatt gacgtcgaag agtttcaacg attcgatgaa gggaacgaat
    11761 ttcttgtagc caaaattgcg gacgtcgaag tcggagaact gcttgctcag gagatttccc
    11821 agggtacccg agaaaatcca gccgttgtcg tcggaatttt cttcggtcag cttgatgagc
    11881 gcctgacgga tggtggagaa atctttcccc ggtgtcttgc cgtggagggg catcgtttcc
    11941 ggcggtgtca gcggacgggc tgccggcgtt tcgctggcat gattatcggc ttccggcttt
    12001 tcggccttag cctgcggact ggtatgttcg gcctgggctt cttcatggtc cgtgccttcg
    12061 tggtcaccgg cttcgtgttc ttcggcttct tcccaaagga cgtcgaggta gaggaacttg
    12121 ttgcaggccg agatgaagga ccggggcgtc ttgctttcgc ccataccgag gacatatttc
    12181 tgcgattccc gcaggcgcga agccaggcgg gtgaaatcac tgtctgacga gacgatgcag
    12241 aagccgtcga ggttgctctg gtacaataag tccatcgtgt cgatgatgag ggccgagtcg
    12301 gacgaattct tgcggattgt attgctgtac tgctggacgg gctggatggc gttatctaaa
    12361 atggtattgc gccacgacgc catctggggc gtcgtccagt tgccgtaaat gcgtttgtag
    12421 atgacatcgc cgagattatt cgcttcagat aagatgacgt cgatgtattt gctggaaata
    12481 ttttcggcgt cgatgaggac ggcgagtttc atttcttgat tcataatttg attacctctg
    12541 tttacattac ttttttatta tatcatcatc cataataact gtgctataat aaaaccaagg
    12601 ctggaacgaa ggcgtttatc cttcaccagg cctatttttt ttaagaaggt gatacgcgtg
    12661 aaaatggaac atatggcgcc tttctgcgtc gtcgcagccg gtatctgctg gggcatcatc
    12721 ggcctgttta cccgccagct gtcggcctat ggcctgtcgc ccttacagat tacctttggc
    12781 cgggccctgg tcacggccat cgccatggct gtcgtcgccc tattccgcca gccccgcagt
    12841 ttggccattg cgccgcggga tatctggatg ttcctgggga ccggcctggg gagcatcgtc
    12901 tttttcaata tctgctactt cacagccatc gaattgacga ccttgtccgt cgcggctatc
    12961 ctgctctata cggcgccgag tatcgtcatg gtcttttcta tcttcctttt ccatgaacgc
    13021 tttacgcggc gcaaggggtt ctgcctggtc ctggcctttg tcggctgcgt cctggtcagc
    13081 ggcttaggca tgggtcatgt cgaggctgcc ggtatcctgg ccggtctggg cagcggcttc
    13141 ggctacgccc tctattccat ttttggccgc tatgccctgg cgaaatatga acccctgacg
    13201 gtcacggcct ggaccttcat catcgcgtct tcagggctcc tgcctttctg ccatttcggc
    13261 atgatccttt ccctggccgc cgccgtgccc tatacgggcc tcctcttcgc ccttctcggc
    13321 ctgatgtcga cggctctgcc ctttggcctc tatacgatgg gcctgaagca tatggaagcg
    13381 ggccgggcat ctatcctggc ttcagtggaa ccgttgacgt cgaccgtcgt cggcattgct
    13441 attttccatg aagccctgac cctgaccggg gcggccggca tggcctgtat cctgggggcg
    13501 gtcatcctcc tggccaggtc cggatccgct aacggatgaa ttcaaagcgg aatttccggc
    13561 ccggcgtgat atagtcgagg agaaattctt cgttcggcac gactttggcg acgatgttgg
    13621 tccgctcttc cggcggcagt tcacatttga cgatctggac ttcgcccata taacgcagga
    13681 aatcggcgtt gtccagggtg atgtcgccgc ggtggagact gcggaagatg tcgctgtccg
    13741 gttcgatgat gcggccgtcc agatgggccc ggctgccatt ggcgcggatg acatcccgcg
    13801 acgggtcggg ccgggccgtg aaggtatagg acaacaggtc ctggatgtgg ggttcccggc
    13861 tgaggagttt ggccttgatg acgacgacgt ccgtttcttc ccggccggcc cgggccaggt
    13921 cgcgcagttc tttatcggct ggcttgtcgt cgccgatgaa gacggactgg acgcccaggg
    13981 cggccaagtg gcgggctgcc aggctgacgt cctgatggcg gtggaattcc agcgtcggca
    14041 ggccttcata cagcggtgcc cgttggccgg cctggctggc gacgaaggcg ccgacggaca
    14101 ggccataggc gtggagcctg tccgtctggg tcttgaaata gtcgagggcc aggcccgtgt
    14161 ggggacgggg atagaagttg tgcaggctgt cgatgtggtc cagctgggcg ccattcttct
    14221 ggaggtcttc caggtctttc tgggtcagcg tcgacgcgtt gacctggacg cggacgatgt
    14281 ggctgtacat ggccagcttg gctgcatcga aaccgtcgtc aaagcggaca gttgtaatac
    14341 ccagcttggc gataacttcc ggatcgaaac ggtccatgcc caggcaggct gccgaggccg
    14401 gcgatacgtc ggcgacgatg tcgagctgac attgccgggc cgtgtccagg acggcctgca
    14461 gttccttgcg gaaggctgcc gggtccgttt cagggatgtg gaatgacgta aagagccgcg
    14521 tcatccccag ggcagcggcc cgttcgatgc gggccgtttt ttcttggatc gtccctccca
    14581 ggccgggata gacggaaata cccgtttcca tttattcacc ctccttcggg tcgtcgaaac
    14641 ccaagagcca ggttgcaatg aagccggcca cgtaggctgt gacgagtcct aataaataca
    14701 tggggatgtt gtccgtactg gctgccagag gcaggccgga aatgccgatc gtcgcggcgc
    14761 cgaccatgta ggcggcctgg acggcgccgc caaaggctcc gccgatgcag gccccgataa
    14821 agggcttgcc caggggcagg gtgacgccgt agatgagcgg ttcgccgacg cccatgaggc
    14881 cgacgggcag ggccgaggcg atggtcttgc gcagggcctt gttcttcgtc ttggcataga
    14941 cggcgatagc cgcgccgacc tggccgccgc cggccatggc caggatgggc aggagaatgg
    15001 tcacgccgta gcgcgacagg agttccgcgt ggatcggcgt caggccctga tggatgccga
    15061 ccatgaccat gggcaggaag gtcccgccca ggatgaagcc cgtcacggcg ccgccggtcc
    15121 cgatggattc cgtcgccgca tagccgatgg cttctgacaa atagccgccc aggggctggc
    15181 agaggaagag ggccacgaag gtcgaaatga cgacgaccag gaagggcgtg acgaagaggt
    15241 cgaacatatc cgggacgagg cggtggagct tcttttccag ccacgatgaa aagaagacga
    15301 ccaggaggac ggcgatgatg ccgccccggc cagggaccag ggattcgccg aagagattga
    15361 tctgagccag gttcggatgg ctgaggatgg ctgccatgac gccgcccagc atgggcgagc
    15421 cgccgaattc cttagccgca ttgatgccga cgaagatgtt caggccataa aaggcggcgt
    15481 tgccggcgat ggccaggacc tgcatcagcg gctgggccgc cagggacggg tccatcttga
    15541 aggccacgtt gagcaggccc gtgatcaggc cacaggcgat gaaggccggg atgatgggca
    15601 cgaagatatg ggcgattttc ttcagggcca gtttggccgg cgtcgcgttc ttgcggcgta
    15661 tttcttcgtg aagggcctgg ccgtcgaagg gcccctgctt atcgtcgccg gcatctggcg
    15721 cagcggccgc cggctgttct ttacggcctg cttcgactaa gggggcaaag gcctggtata
    15781 gtttggtcgc cttgcccggg ccgacgatga tctgccattc gtcaccggat ttgttgatgc
    15841 ccatgacgcc gggcagtttg gccaggtcgt ctttgctaaa gtgttcatcc tttacggtca
    15901 ggcgcaggcg ggtcatgcag ttgtaagcct tttcgatatt gcctgccgga ccggtgatat
    15961 cgtagatggc ccgtgccagt ttatcgtaat ccatgggcag cctccttgag gacggcggcg
    16021 acatagccct gggccgctgt caggcggtct tcggcttcgc tggccgggat gtgcaggagg
    16081 accatgacga tggccgtctt ggcccggccc tgactttccc tgagggcttc gatggccttc
    16141 tggcggctgc agcccgtcgc cgtcatgacg atgcgtcgcg cccgttcttc caatttctga
    16201 ttggacgact tgacgtcgac catgaggttg ccatagactt tgcccaggcg gatcatggcg
    16261 cctgtagaca acatgttggc gatcatcttc tgggccgtgc cggctttcat gcgcgtcgag
    16321 cccgtgacga cttccgggcc gacgacggcg cagaggtcga tgtcggcaca acggccgatg
    16381 gccgaatgag gcgaacagtc gatggcgatg gtagcggctc cgaccgaggc cgcataggtc
    16441 aggccgccga cgacataagg cgtccggccc gaggcgctga ggccgacgac gacgtcgtca
    16501 gccgtcaggt ttttgtctat caaatcctgc cggcccagtt ccggcgagtc ttcagcccct
    16561 tctttggccc ggaagatggc ttcataaccg ccggcgatga ggccctggat gcgttccggg
    16621 tcggtgctgt acgtcggcgg acattcgacg gcgtcgagga tgcccaggcg gccggacgtg
    16681 cccgagccca tgtagaacag acggccgccg cggcgcagtt tatcggcaat ggtgtcgacg
    16741 gcttgggcga tggccgggat gatggcgccg acggcatcgg cgactttatg gtcttcttca
    16801 tttatgatgc gcagcatatc ttcggtcgat acgctgtcga tatgctggct atggctgttc
    16861 tgctgttccg tagccagggt ttctaaattg atcatgcttg attcctttct ttcatgcagt
    16921 cgtggctatt gccgcttggg aacaatatac acatctctaa ttatagagag tgtttgaaag
    16981 acttgcaata ggcctttttt tcatgagcca gcacctttat ttttttgccg tttcatggta
    17041 tgattaatct gcacgcagca gacctgtgtg ccctgtgcgc ccatagctca gctggataga
    17101 gcagcagttt cctaaactgc gtgtcggatg ttcgagtcat cttgggcgca ccattttttt
    17161 atcttgaata caatctaatc attttacaat ccggaaaggt gggttctctc atgtccgata
    17221 ccaagaaccg gctggcttct ttccatgaag aagtcatgca tgatgctgat tatacgctgg
    17281 ctgaagccgt tgccgaagcc aaacgctgcc tgaattgcaa agtaccgcag tgccgcaaag
    17341 gctgccccat tgaaaatgaa atcccccagt tcatacatgc catgaccttg ggcgatttcg
    17401 gcgccgctgc tgaacatatc tatcaccgca gtgatttgcc ggctatctgc ggccgcatct
    17461 gtccgagaga aaagcagtgc gaaggccact gcatcctcgg caaaaaaggc aaacacgtcg
    17521 aaatcggcaa actcgaacgc ttcgtagcgg acatggccct ggatggcggc ttcctgccgc
    17581 agccggtcag ccgtaaaaca gaaggccgcg tggccatcat cggctgcggc ccggccggca
    17641 tggcagctgc gagagaactg gctggccgcg attatgatgt aaccatcttt gaagccgctt
    17701 cccagcccgg cggtaccatg acctatggta tcccgacttt ccggctccat aaggaattac
    17761 tgcagcgcga agcccaggcc ctgacagcta tgggcgtcac catcgaatat aacgtcaaaa
    17821 tcggcgtcga caaatccctg gacgaactgc agcgtgattt cgatgccgtc ttcatcgcca
    17881 tcggtacgat gaatgcctgg aatctcggcg tcgacaacga cacgatcgac ggcgtcgtcg
    17941 atgcggaaca gttcctgcgc gacgtccagc gcgtccagac cggtgaagtc gaactagcag
    18001 acctgcccgt acagaaaggc gaccaggtca tcgtcgtcgg cgccggcaac gtcgccatcg
    18061 atgcggcccg cacgtcgctg cgcctcggtg cggacgtcaa aatcgtctac cgccgggctg
    18121 aaaagaacat gaagtgcctg ccgtcggaat acgaagaagc caaggccgat ggcgtccagt
    18181 tccagttcta ttcggcaccg aaagccgtcg tcgggacgga ccatgtagaa ggcctgaaat
    18241 acgaaaaaca gcagatcctc gaagatgcca ccatggtacc gacgggagaa ttcggcgtcg
    18301 tcccggccaa taaaatcatc gccgccatcg gcaacaagcc ggaattgtcc gtcgtcaaag
    18361 ctctcggcgt cgatgccaat gacgatggct acatcgccgt caaggatatg ccttacggcc
    18421 tgaccagcaa agaaggcgtc tttgctgccg gcgatatcgt ccacaagccg cagaccgtcg
    18481 tcctggccat gcgcgaaggg cgtaagactg ccgaaggtat cgaccagtac ttgaaggcca
    18541 aaaaagtcat ggccgctgtg aaagcatgag gaggccgcta tgagtgaatt gacacggcag
    18601 atactgaaag ccaataagtc ttttgtcaaa cagtccctgt cccagggcgg cttcgatgaa
    18661 gtcagcaagt atccgagccg caacctggcc gtcctgacct gtatggacac gcgcctcttg
    18721 aatttcctcg aaccggccat gggcatcgcc cggggcgaag ccaaactcat caaagtcgcc
    18781 ggcaatacgg cttttgaaga ctttgacagc gtcatcggca gcctcatggt tgccgtctac
    18841 gaactgcacg tccacgacat catcgtcatg ggccatgacg actgcggcat gctcaagacg
    18901 acggcagaca gcctgtgccg ccatatggcc gaagaaggca tcgatgaaaa ggccatcgcc
    18961 gccgtccgcc ccaaattgga acagtgggcc gaccccatta ccgatatcga cgcgtctgtc
    19021 tgcgatacgg tccgccgcct gcgggccaat ccctacttgc cttcatccct tacgatttat
    19081 ggtatggtca tccatccgca caccggtgaa atccgcgtcg tcgacgatgg agaaggccag
    19141 gaacaggagg tattatgagt ctggtcacac aacgaatgaa agaccacgcc gccgtcctcg
    19201 aggcgacgat ggagttgatc ccggatattg aaaaagccgg ggctctgttc aaagaggccc
    19261 tggcttctgg tcacaagata cttttttgcg gcaacggcgg cagtgctgcc gattcccagc
    19321 atttagccgc ggaaatcgtc ggccgcttcc aaaaggaacg gcgcccgttc ccggccatcg
    19381 ccctgaccgt cgacacgtcg atcctgacgg ccatcggcaa tgattatggc ttcgatacgg
    19441 tctttgcccg ccaggtccgg gccttaggcg aaaaaggcga cgtcctggtc ggcatttcta
    19501 cgtccggcaa cagccagaac gtcctggaag ccatcgatgc agcccgtgaa aaaggcctca
    19561 ctgtcatcgg cctgaccgct tatggcggcg gcaagatgaa ggaggcctgc gatatctgcc
    19621 tggccgtacc ggccaaagtg acggcacggg cccaggaaat gcatatcatg attggccaca
    19681 tcctctgcga aatcgctgag gaagacatgt aatcagccga cgatttgctg gaactgtttg
    19741 ttgttccgta tcttatcgaa atggctctgc gtccgggctg cctggcggac gtcttcgccg
    19801 cccaggtcga tggcctgctt cagatagggc agggcctggt cggctttgcc caggtcgccg
    19861 tagatggtgg cgatgccgta gtaactccag gtattgcccg ggtcggcggc gatgacgttc
    19921 ttgaaatacg tcagggcctt gtcgtattgg ccgtcaatct tataggctaa ggccaggttg
    19981 tagaaagaag cgacgtcttt cggggctatt ttcagcgctt tttcgatgag ctcgatgccc
    20041 tgggagacgt cgtttttgct gcctgtgctg ttgccgcgca tggccagggt gacgccctta
    20101 ttgctcaagg cctgatagtt gttggcgtcg agggaaaggg ccttgtcgaa atcgttcagg
    20161 gctccggcat aatcgccggc ctggtatttt ttcaggcctt cgtcgctgta agcctgcgaa
    20221 tcggcctgga tggccggtgc cgatggagcc tgtacgtcct gtgccgtctg agccggctgg
    20281 ggctgggata gctgtgtact gcatccgccg ctcccggcag ccagggccag caggagcagg
    20341 atggccaagt gtttgttctt catgttcatt ctcctgttcg tcaatatgga aatcctatga
    20401 aacatatcat caaaaaaggg gacatcgtcc tcatcattac gctgctcgtc ttatctttca
    20461 tcccggaagg catcttttac ctgaccggta acgatgcgtc tgtgagccgg acgtatgcgg
    20521 tcatccaggt cgacggcaag gtctataaag aagttccctt gtcgggccac catgggaccg
    20581 atgtcatccc tatccagacc gaggagggct ataacctcgt ggtcatccag gacgagagcg
    20641 tcggcattac cgaagccgat tgtcccgata aaatctgtat cagtgaaggc tttgtcagca
    20701 agcccggtgc gaccgtcgtc tgcctgccgc acaaggtgct ggtcgaagtg aagtccgccg
    20761 gcgatgacga acccgatgtc attccggccc attagaggta cttatgaaaa agacatttca
    20821 cattattatc ctgggcctgt tcatcgccca gtccctggtc ttatacgtcg tcgaagggat
    20881 gctgcccgtg cccttcatcg cgccaggggc gaaactgggg ctggccaatc tgattacggt
    20941 catcgccctc tatgccctgc cgcgtaagcg cgacgtctgt ctcatcctgc tcgtgcgcat
    21001 catcctggcc acggcctttg gcggcggcat caacgctttc ttgtacagcg ttgccggggc
    21061 ggctttcagc ctgggcgcca tgatggtcct gcagaagacg gggaaattct ctatcatcgg
    21121 tgtcagcacg gctggcggta ttttccacaa cctgggccag gtcatcgtgg cctctttggt
    21181 cgtagagaat atcaagatca tgctctacct gccgatcctg gccgtcgccg gcacgggaac
    21241 gggcatcctc atcggcatta cggctatttt caccttgcgc cacttgaagc ggctgcccat
    21301 ttaccggcgc atgcaagagg aaaatcaggc gattaattga ttcggatgga gttgagtact
    21361 tcgtcagcga gctttacctt atcttctttg ctgccgtcgc aggctacgac gacgatccac
    21421 gtcgccgggc cgtcggcaaa gccgatgagg cgggccttca tggatttatc ctgatacgtg
    21481 aaggtaaagg tctggacgac ggcgtgctgg ccgctgaccg tcgtcgtgtc ctggctgacc
    21541 gagacgttct gggcgtcgat tttcgataat acggcgccgg ctgcggcttc ggccgtctgg
    21601 ttgaggcgct ggctgatggc ctggccctgg ctgatgtgca ggtcgtcgta catctgctgc
    21661 gtgtcgatgc tgttggtata gatggcgatg tacaggccgc cgtccatttc accgtacagg
    21721 cggctgccct cgaagtaccg ctgggtcagc gggtctacgg ggacggggaa gtcgagcttc
    21781 tggagatttc ccggcagttt cagggaaatg gccgagccca tgccgtcgac gcggcgttct
    21841 ttgagggtaa tcgtatagca ggccgtcagg ctcagggtaa ggatgagcag gcataaagcc
    21901 agacagaggg aacggattcg ggtatgagtc atgagtgcac ctcgcgaaag ttatttgaaa
    21961 ggcaggtatc cccaggatac gagcaggtaa aaagcgggat atagaataag tatatataag
    22021 agctgtgtcg taatgaaagg cgccatggcc agattgacgt tcaagtcgta aaggcgctcc
    22081 gtaatcaagg cgttgatggc acagggcgta gccatgacga tgacaatcgt ccccaggagc
    22141 accgggtccg acgtgaagca caagaccagg aaatacgatg ccagcggcgt caggaccatc
    22201 ttgatgggaa tgaggtccag cgtctggcgg gcatagtcct tgaagtggga aaagtcgatg
    22261 aggtagccga cggggatgag ggctacccag gcgctgacgt ggatcagggc ctggaaaatc
    22321 gtcgtccccg cagcgggcct ggggatgccg gcggcgtgta aggccgtgcc gatgaccagg
    22381 gctacgacgg acaactggtt ccagttgatg aagatcgatt tccagttatc cttgaaaaag
    22441 acgaagaaat tgcggtcctt gccgccgtgc tggtagtagt agcccatggg gaagaggacg
    22501 aagaacatga gcaggttctg gaagacgccg atgatctgga cgtaggcata ggacatttcc
    22561 ccgtagagaa tgtagctgca caggccgccg agcatccccg tgttggccgg gatggcggcg
    22621 atgaggtaac tgcctcggtc gacgaaggac gagaatttgc gctgccgtcc cagcaagagg
    22681 acgacggcca gcggcaggag gccgttgaaa aaagcgaaga aaggcaggct gagcaagtcc
    22741 gtccgcatgg gcaggaccca gaagctgagc agggtcaggg acgtcatgat gacgatgatg
    22801 ttgaagcgga tgagccagtt cgactggtcc ggcgtcagcc agctccgcct tttggcgaca
    22861 tatccggcaa tcatgggcag gatgatgtct gtgaaaatat aaaccatccg gagtgtcgta
    22921 tcttccatgg ccggattccc tccctttact tgcgggcagc gcggaaggcg tcggccaggg
    22981 agaacgtcag cggttctgcc ggcttggtgg cgtcgatgat aggctgcatg atatcgcgga
    23041 attcttcttt tttcttcgat aagacgtaga gtttggacgt gttgcgggca tcccagaggg
    23101 cgtcgtgcat ctggccgtcg aaggtttctc ccagggtgct gacagccttg tccagggaaa
    23161 tgacattatc caggcccagg aggttgcagt aaatctgctg gaagttctgc cagtggtcga
    23221 gcatacagcg gacgcggtgg tcgtgctcgt cgatttcttt gaggcggagt tcgcagtcca
    23281 gctggcgcca gtcattttca ctccaggcga acatttcgta ggcattgggg cagccttcgt
    23341 tgcaccaggt caggaaatgg tcgaaggcct ggcggaaatg gggtttcccg gccagcattt
    23401 ccatggtgat acctgtcaat tcctgacagg tctgattcat ggctgtcgta tattcgggca
    23461 gcacatattc cttatacgac gagatttcat tcccgtcttc gtctattttg acagcaccga
    23521 tttcgatgat ttctcctttg cagatgcggc gcgcttcctt ctggtcccgg ccgatagggt
    23581 tcatctcgaa atcgacaaat acggctttca tctaaatgcg ggcaggatac tccgacctct
    23641 taggtcggag agaaatgccc gcctcacctc actttcttat taagtagaat ttcagcaaaa
    23701 aacaatgtta taatattttt gatgaagcaa tatacatttt agaaaggcag gtgagaatat
    23761 gtccgaccta accaaaacga taaaacttcg catacatgtt actcctgaac aggaagtatt
    23821 gttccgtcaa atgacggagc agtaccgtca ggcttgcaac ttcgtgtctc aatacatctt
    23881 cgacaatcag ttcgacttga cttaccaaag cctcaacaag aaactgtata gtggnnnnnn
    23941 nnnngtggct tgttcggctt gaagtctcaa ttagcccagt cttctatcaa gacgacgatt
    24001 gccagataca agacggtcaa gcaacagtta ttccgaaagc cttatagata caaggatgag
    24061 gatggcaact ggcaacgcat ccctaagact ttggaatggc tctggaaacc cgtattttat
    24121 cgccggccac aggctgactt agtccgcaac cgagactaca gcttcgttga tgacgggcaa
    24181 gtcctgtcca tcaatacact cgggaaaaga accaaatgta ccttcgaagg cgaacatttc
    24241 gctggatacc ttgatggttc ctatcaactc ggtactgcca aactggtcga actgaaaggg
    24301 agatggtatc tgcatatccc tgttaccaag gcggtcgaag atttccagaa ggagaacgtc
    24361 cgccatgtcg tcggcatcga ccgtgggctt cgtttccttg cagtcagcta tgacgaacaa
    24421 ggcaagacgg agttcgtctc tggcaaaaag attgccacca aacgccataa attccaagaa
    24481 gtacggaaac agctccaggc caaagggaca aaatcagcga agcgtaaact caaagccatt
    24541 tctgggcgag agaaccgttg gatgtctgat gtcaaccatc agctatctaa gacactcgta
    24601 cagaaatacg gcaaggatac gcttttcgta ctcgaggacc ttactggcgt cagcttcgag
    24661 gaaagcaatc tttccagaac cgcgaaacag aactatgatt tgcggagctg gagtttttac
    24721 cagttggaac agttcctgac atacaaggct cacgagaacc gttcggaagt cctgaaagtg
    24781 tctgcgaagt acacctccca gcgttgcccg aaatgcggga ctatccgcaa ggcgaaccgc
    24841 gaccaccata agcatctgta cagttgccag tgcggatacg aatccaacga cgaccgtatt
    24901 ggagccatga atatccagct tctcggaact atgtggattt caggggacaa ccatcctcga
    24961 tatgaacgag taatgactac cttggagtaa actccttggt aaagcgggtg ttgtcaatca
    25021 cccgacgatg taggcggata tagggagaca tcttggatgc cgttagcacc tatgcttttc
    25081 cgacctacaa gctcccacct cgaacgccga aggcgttagg tgggggtagt tgacaagagt
    25141 gtctcctttc tgaatctgat gacaaccatt attataccat aagtaaaggg cttatcgcac
    25201 gtgcgacaag cccttttaag aggattcagg cgggccgccc ttggggcggc ccctacgaac
    25261 cactagccac tagccactaa aacgccggca ggatagttcc ctggaagtgt tcttcaatga
    25321 atttcttgat cggttcagac tgatagattg ccaagacttt cttgtatgtt tcgttgtttt
    25381 cgtcgccctg acggactgcc atgacgttgg catacggcga atccttcgat tcgacgagga
    25441 tcgaatcttt ctgcgggttc aggccggccg ggatagcgta gttggtgttg acgcaggcca
    25501 ggtccgtatc gtcgagggag cggggaatct gagcggcgtc gagttcgacg aactggaggt
    25561 tcttgggatt ttcggtgata tctgttaccg aagcgtcgac agagttgccg ttcttcaatt
    25621 taatcaggcc ggcctgctgg agcaggagca gggcgcgggc accgttggtc gggtcgttcg
    25681 ggatggtgac tttggcgccg tccgtgacgt ctttgatgtc tttgaattta tgggagtaaa
    25741 cggccatggg caggataatg gtcttgccga tggaggtgat cttcaggcct tgtttcttga
    25801 cgacgttgtc catgtacggc tgatgctgca tggagttcat atccaggtcg ccttcggcca
    25861 gggccttgtt cggctggacg tagtcgttga attcgacgac tttgatagta atgccctgtt
    25921 tagctgcttc tttagcgact tcttccatga cttcggcatg aggaccggca gtgacgccca
    25981 tcttgatttc ttttttgtca ccggaagcac cggaattgga agacgaaccg cagccggcgg
    26041 caaagaggca cgtcgtcgtg atgagaacgg cggcgaagag tttttttact gttgcgttca
    26101 tgtaatcatt tcctttctgg ataacggata ataagggctt gccggtcaaa aagaaaagcc
    26161 gtcatacaga tgacggctca atttgcgatt cgttcatctg ccggtataca ccgctggact
    26221 tggcacctat cccgataggg ggttgccgca gcgtcatcgg gccattccct cgactgctct
    26281 tgatgaaaga caatgtatgg ttgttggtgt acatattatc acctggaaga gaatctgtca
    26341 agaaatattg tgcttcttga ggaaaatttc tttttatgtt ataataatac cgctcatcgg
    26401 aggcttatgc tcgtatagta taaagccgga taagatgtca cttcagtcct gcagcggagg
    26461 aagggcgcgt acgtaaacgc gtctttcgat aaagatgcag gacagggtgg tttcttgtcc
    26521 ggcttttttt agtgtgaggt gacagaaatt gatgaataaa cgagatatcc tttggcagat
    26581 ttatagtgcc aaagcctttt acttcctcgg cggtttcggt tttacgtcgt gggcatcgct
    26641 cattcccttc ctgaggcagc agctgcagat tcaggacgac cacctggggt tcctcttatt
    26701 ggccgtcggc ttaggcgccc tgatcatgat gatgctggcc ggttccattg ccgggcgcct
    26761 gggctgccgc cgttctttga ccatcgccgg cctggccatt gccgtcgtcc tgaacgttct
    26821 ctgttatgtg ccggcttatg cagcggcctt ggtcatggcc gtcctcctcg gttcgtccct
    26881 gggactgctc gacgtcgtcg tcaacatcaa cggcatcttc attgaacgga aagtccacaa
    26941 gcgcctcatg tccggcctgc aggccatgtg gtccctgggg aatttcgccg gcgctgcctt
    27001 ttttgcttcc ctgctccacc tgggctttta ctggaaaatc gtcatgattg ccggcgccgt
    27061 cttcatcggc ctctgcgtct gcttctttgc gccccatctc catggggaac ggcaggcatc
    27121 cggcaagggc gcttccctgg tcctgcccaa ggggaaaatc gtcctcatcg gcctggtctg
    27181 caccattgcc ttcctggtag aaggttctat caacgattgg agcggcgtct tcatgacgac
    27241 ggaaaagggc atcgacattt cccaatccgg cctgggcctg accctgttca cggcgtcggc
    27301 cttcctggcc cgcctgcctg gtgattccct gactatgcat ttcgggccgc ggcggctgct
    27361 ggccctgtcc ctgcccgtcg cctttgccgg cttcctgggc atcctgctca tcagcggcgg
    27421 ccccttcctg tttgcatcgt acgtcctcat cggcgtcggc tgtgccaaca ccgtgccgat
    27481 cttttattcc tctctgggga cacagaaaga catgcccgta gctgatgctg tcgccgccgt
    27541 cagcactatc ggctacgccg gcatcctcct ggcaccggcc gtactgggct tcatcggccg
    27601 ggccttcagc ctgaccgcct ccttcgcctt agtcacggcc ctcctggtca tcatgtccat
    27661 catggccatg cggctcttgc gggggaaata gtttgtagtt tgtagtcggg gatgtgtaag
    27721 aatcccgtga ccgacttgcg taaacgggcg ccccacgtgg gcgcccctac taacccctat
    27781 cccctaaccc ctaaccacta accactagcc actagccact taaaaagggc ttgtcgaatg
    27841 acgacaagcc ctttcttcac gcctcaatca ctttccccgg gttgaggatg tctttcgggt
    27901 ccatggcccg cttgatggtc ttcatgattt tcagttcgcc ttttggcgtg aactgttcca
    27961 tatatttcag cttcttggca ccgatgccat gttcaccggc caggcggccg ccgacgctgt
    28021 aggcataggc gtagacctgc tggtggaatt cttcgacgtt cttttcccat tcttcgtcgc
    28081 tcatatccat cttgcagagg acgatgtgca ggttgccgtc gccgatatgg gcgttgatct
    28141 tgacttcaaa aggatagttc ttaccgatgt ccatgatttt ttcaatggtc ccggcgattt
    28201 tatgtaccgg aacgacgacg tcgtccgtga ggaagacctt gctctggagt tcgacggacg
    28261 tctggcagtt acggcgcata ttccagacgc ggtcgtcagc ttccaggact tcggcagcgc
    28321 ccgattcttc acagatcgtg cagacagcgt ccatcttggt gtccaattcg tcttcgtgga
    28381 acgtttcgac ggtgatgatg acgtaaatgc cgtcgtcgta gtgcggcatg tccttgtaat
    28441 ggcagtagtc agcgcagccg cggacgtagg aattgtccat gtattcgacg ctggtcgggt
    28501 cgatgccggc tttcatcagc ttcggcacca tgtgcagggc ctggcgcggg tccgtgtaga
    28561 tggccaggac gtcgaagcgg tacggcggca gggggatgag cttgactgtg actttggtga
    28621 tgatgcccag cgtcccttca ctgccgatga tgagctgttc caggtcgtag ccggtagagc
    28681 atttcttgag gatgcggccg cattcgacga tatcccctgt aggcgtgacg atttccaggc
    28741 agtagacttg gtcgcgggtg acgccatagc ggacggcgcg caggccgccg gcattggtgg
    28801 ccaggttgcc gccgatgagg cagctttcgg cgctggacgg gtcgccggca tacatgagac
    28861 cgactttttt ggcggcgttc tgcaagtcta ccgtgcggac gccggcttcg acggtcatgt
    28921 acatgccttc ttcattcagt tcgatgatct tgttgagacg gtccatgcag aggaccaggc
    28981 cgccgcagac agggatggca ccgtcggcca ggctggtgcc gccgctgcgg acggtaatgg
    29041 ggaagtcgta ttcattggcc aatttgacga tgtccgagat ttcctgggca ttggccggtg
    29101 atacggctgc ttccgggaga tggaacatgg ccggattggt ttcatagtct gtctgatact
    29161 gtaagagcag gtctttatcg gtcttgacat aagtcggacc ggtaatggcc tttaattttt
    29221 caatgatttc tgccgtaata ggctgataag ttttcaagcg aagggcctcc ttataaaaat
    29281 cggttgtctt attttaccac gcccgctttg cttagggaag gcatcagaac ttgaaatcga
    29341 ttgttgccga gacgccgacg ccctggacgc ggctgaggtt ggtgacattc gtcgtcgcca
    29401 ttgggatgag ggcattggcc cgggcgatgc cgttgaattc cccttccagc tgagcgacgg
    29461 ccttgacctt ttcgacctgt gccgatgggc cgatgacctg gacggcgccg atatatttac
    29521 ccgtaccgac actgacgatg gggacgactt tcgtcgcgta gtccgtgccg acgccgttct
    29581 tcatgagcag tttgttgagg aaatcattga tggaatcgcc gtatttggaa acgaggacgg
    29641 aaatgccgcc gactttcagg atagagccga ggttgaaggc ctggacggcc gtgccaccgg
    29701 caaagccgag gagcgatgca accgtaacgg ctgcgatgag ttgttttttc atggaaatac
    29761 ctcctaattt acactttttt gggacttgtg ccgcgggcca gcgaggccgc gacggcgagg
    29821 aaacagagga cggccaagat gacgaacgaa gcgtgcaggg cggctacgac gccatccgca
    29881 taagacggct gtttggccgt ctctgtcgtc atgatgaagg tgacgacggc catgctcagg
    29941 gcctggccga ggttgcgcga taaggccagg atgctcgaag cgatgccgtg atgcatcttt
    30001 ttgaccgacc ccatgatggc gctgttgttg ggcgcgccga acagggctgc gcccaggccg
    30061 atgaagacca ggaatatggc ggcgccgctg acggggatgt gcgggaacag ggcgaatgcc
    30121 gtcagcccca gggccgtcag gacgaggccg atagaggcaa tgccccgtgg gccgtattta
    30181 tccgacaagt cgccggcccg gggcgacaag aaggccatga tgatgggctg gaccaggaga
    30241 atggctccgg atacagccgg cggcaggccg aggatgacct ggaaatagag ggataacagg
    30301 aaggacacgg catacgtgga gctgtactgg atgagggcag ccaggttgga catggaaaaa
    30361 gtcaggttgc ggaacagata gagtggcagc agggggtgga aggacttgcc ttcatggatg
    30421 acgaagatga ccagcaagac caggccaatc cagaaaaaag cactgtaatc ttcaaaggat
    30481 gatagcccgt acagcgcgca gaggatgccg ctgaccgata aggctgtact gaccaggttg
    30541 acaaaaggcg cgccgttggc ataccattct tctttgatgg gccggatgaa ggcgtacgaa
    30601 atgatgatgg ccagggcaga caagaggaag atacagcgcc agccgacgaa ctgcgtcagg
    30661 gcgccgccga tgaccggacc cagggacagg ccaagatagg tcagggcaac ggcccggccg
    30721 aggatatggc cctgatgggc ttcatccgtc gtggccagga gcaggggcat gtaagatacg
    30781 tagacgccgg acagggcgat gccctggatg gcccgcaaga tgatgagcat cgtcagggtc
    30841 tgggcgaagg ccgccgccgc tgtcgccatg gcaaagacga tgagcgacag ggaataggtc
    30901 cgccgccgtc catggacatc ggagaccttt cccaaaggca ggagaaaagc agccgtcgtc
    30961 atcaagaaaa tcgtcacgac ccagctcatg cggtccggcg cgacgccgaa ctgaccggcc
    31021 atgacgggga tggcgatatt gatagacgta gaaagaaaag gcccgacaaa actgatcagg
    31081 caaatcgagt aaaaaatccg tttttccgtc gacatatccg cacctcacaa acgtaatcat
    31141 tctgctccta ttataccata tgagccggag ccggtgggag ctggcggaga aaatcgcgga
    31201 ctaagaacgt gcagaaataa ggaaaggtgt aaatatcgtt ttcatggccg atgttgccgc
    31261 cgatgagctt gatgccgaag cggatgtcac tataagacgg gcttttcgac gaaaaacggc
    31321 tttttctgag gacgctcttt ccactgaatg aggaagtcat cgatttttcg ctttaaaagc
    31381 agtatctgag cgagtcaaaa taatgtttgt tataatgata tgctaacgtg aatttttttt
    31441 gctaatttat tagaaaatat ataaaaagat gaaactatac ttgacaagta gccttttgaa
    31501 tagtaccata ggcgtaaaca ttgagaaatg cgataaccaa aacaagtaca tctgtgtact
    31561 gcgtcagaga gtgcaggtct ggggctgaaa gcctgcatcg ttgggaaaca ggatggaatg
    31621 cctttggttg catggctgcg gaatttagta tgcagctgcg tctgaccggc gttacacggt
    31681 tatgagatac agttttggct gtaaacagag tggaaccgcg ggccaccgtc tctgtcatag
    31741 gagatggtgg cctgtttgta tatccacaga aggatataga gaaaatgaga aagaggggtt
    31801 atgaatgata caggacataa atcaatatat tcatgatgaa tttcaatggt tccatcgtca
    31861 tccggaacta tcttttaaag aaatagagac gaccaaacgg atccgtcaag acttacaaaa
    31921 aggcgggctt catattttga atctgccttt ggagacgggc gtcgttgcag aggtcggaac
    31981 aggaaaacat cctattgttg ctatccgctg cgatattgat gccctgccgg ttacggaaaa
    32041 agctgacgta cctcatcggt cagaacatcc cgattgtatg catgcttgcg gacatgactt
    32101 ccatacagct tctattttgg gggcagctta tttattgaaa gcacgggaag ccgatattca
    32161 aggaactatc cgtattattt tccagccggg agaagaaggt ccgagcggag ccttgaaaat
    32221 cattgaaacg ggcattattg atgatgtcga tgcgattttt ggaatgcact gcacgcccct
    32281 tctcgatgtc ggtcaaatgg ggatcatcga tggtccggcc atggcggctg tcgatgcttt
    32341 tcgtattact tttcatggca agggcgtcca tgcagctcat ccggataaag gtatcgatcc
    32401 catcgtaaca gcgtctgctt ttgtcggtgc tgtccagact gtggtcagcc ggaatatgga
    32461 tcccttcgcc gctactttgg tcagcgttac ccatattgaa ggcggtcaca actggaatgt
    32521 cattcctgat gaaatttttg ttgaaggtac gacgcggagt atgagtaaag aagggcggca
    32581 ggctattcgc aatcatatcg aatccttggc ctcttccacc gctgctgctt atggcgccaa
    32641 ggccgaagtc cattggattc cagggccgcc ggcattgatt aatgatgctt attggggaat
    32701 cttagcccgt aaggtagctg ataaacaggg ctttgataaa caaccgatgg ccccgtcact
    32761 gggaggcgaa gactttgcct tttattacga taaggtacct ggttattttg tttttgtcgg
    32821 gactggccat tcctacccga atcacaatgc taaattcaag gtaaatccag ccgcactata
    32881 tccggcttca caatatatgg ctgaactggc ctgccaggca gtagaacaga ttcagcaaga
    32941 agggagcagc aagggatgat tcaatttaaa gatgtcagca agacctttaa aagcaaaggt
    33001 gttacgacac aagcattaca gcatgtatca ctgactattg atgacggaga tatgtttggc
    33061 gtcatcgggt tcagcggtgc aggtaaatcg acgctgctgc gcatggtcaa cggcctagaa
    33121 atgccgaccg aaggtcatgt agaaatcaac ggccaagatt tgagtaaaat ttctcacaat
    33181 gaaatgcgta aacttcgcaa atccattggt atggtctttc agcaattcaa tcttctcgac
    33241 tcgaggaccg tttttgacaa tgtagctatt cctttacggc ttaataaagc ggataaagac
    33301 aagatgcata aaaaggtcat ggaacttttg gaatacgtca atctggcgga taaggccaat
    33361 gcttatccgg gtcaactttc tggcgggcaa aagcagcgtg ttggcattgc tcgcgcactg
    33421 gctacggatc cgtctattct cttatgcgat gaagcgacaa gtgcactgga tccggaaacg
    33481 acggaatcga ttttgcagct ccttgaacgg attcataaag agttaaatat taccatgctt
    33541 atcgttacgc atgaaataca agttattcag cgtctatgca atcgtgtggc cgtcatggaa
    33601 catggtaaag tcgtagaaag aggatctgtg ctggaagtct tcagtaatcc tcagcaggat
    33661 atgactaaaa aatttgtccg tacggttatt cccgaccaga ttccgccgtc tgtcgttgcc
    33721 aagattcatg aagaaaaaga taattataaa attttgaaac ttcgtttcct tggcgataat
    33781 gcgacagacc accttatgta tcatatcaac aaaacctttt ccgtccagac gaatatcctc
    33841 tttgcatcag tcaatgaatt acaaaagacg attcttggta tcttcatcct tcagattatt
    33901 gggaatgatg cagagattgc caaggttatt caatacattg atgaacagca ggttcagtgg
    33961 caggaggttg atttgtaatg attgatttag gtgtgcctat gtcgcagatt attttggcgg
    34021 cagaacagac tgtatatatg gtcttcgttt cgttattctt agggactatt ttggcaatta
    34081 tcatgtctgt tgtcctggtg ctgactaatg atgatggcat tttgaagaat aaatatgtct
    34141 atactgttct caatacagtt atcaacatta ttcgcagtat cccgtttatc atcttgatgg
    34201 tttttatttt gccattgact aaaatgattg ttgggactcg tattgggacg acagcggctt
    34261 tagttccact ggtcattttc attgcgcctt acttgacccg gctttttgaa aattccattt
    34321 tggaagtcag caaggggatc gtggaagcgg cgcagtccat ggg
//
LOCUS       DAWABZ010000003        31661 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_408_length_31661_cov_3.518035, whole genome shotgun sequence.
ACCESSION   DAWABZ010000003 DAWABZ010000000
VERSION     DAWABZ010000003.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 31661)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 31661)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..31661
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_408_length_31661_cov_3.518035"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6616..6625
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7199..7208
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16945..16954
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    26186..26195
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7690 a         9044 c         8591 g         6296 t
ORIGIN      
        1 cgcggcgatc ccagccgcgg ttcgtgctgc cccatggcgg tcggcaacgc cctgctggaa
       61 atgttcgatg aagtccagga agaactgcat cagacggcat ccgtcgccag cggccatacg
      121 ccagtcaaaa agacgccggt ccggccgaaa gctgaaaaga aggtcacagc cgaaagcatg
      181 ctctgccccc agtgcggcga acccttagtc tttgaaggcg gctgcaacac ctgcaaaaac
      241 tgcggctgga gtaaatgtaa ttagagtctg atttcgcctc tggtgacagc cgtcttgcat
      301 catcttgccc cgtttctctt cgtcagccgc tcctcgacgt agctctgcta cgccttgcgg
      361 gcgtcttcct tgagaaacgg ggcaatctga cgcaatccaa ctgcccccag agacggcatc
      421 agactctggg ctgtgggaga tttggaaaag ccaatgagtc gtgccactgg ctgacgctgg
      481 cctggcaatc caactgcccc ccagagacgg catcagactc tatgcaatct ctggaaaatt
      541 gggaaaagcc aatgagttgt gccactggct gacgctggct tttaggaaat gggcaatccg
      601 gactgtaggg gtcgccacgt gggcgacccg ctggagactg atagtgatag cccatggcag
      661 gatgatggga ctgatttatc ggctacgagc acgcacctac ggctgttggg ggatgatcct
      721 gttaaaattt gtcttgacag gcagggcgtt tgtgtttata ataaacgtaa ttttacagaa
      781 ggcgatgaag gaaaagcaaa ttatgggtcc tttcagagaa ctgccgggtg gtgcgaggca
      841 gcagggctgt aaggcgttcc gctctggagc tgcaaatgat gaatttgacg ggtacgcccg
      901 atatagcggt acaagttggc cgaaaggcaa gttgggtggc aacgcgggaa tatcgactct
      961 cgtccctttg atgggacggg agttttttta ttggtatggc agaagacttt ccctgcggtc
     1021 ttcgcgcagt aagaggtgga aatatgaaga gagtgtacaa tttcaatgca ggtccatcgg
     1081 caatgccttt ggaagtcctc aaggaagttc aggatgaatt cctcgatttc aatcacacgg
     1141 ggatgagcat cgtcgaaatc agtcatcgca gtgctgccta tcaggaaatg caggatgaca
     1201 cggtagcgac gctgaagaag ctcctggctg ttccagatga ttatcatatc gtcttcatcc
     1261 agggcggcgg cagtatgcag ttcgtcatgc atgcagcaaa tttcctcaag aagcgcggcg
     1321 gttatctgaa tactggggta tggtccaata aggccatgga agccgcttcc ttctacgggg
     1381 aaacgtatga agtcgcttcg agcaaagccg atggctttac gtacatcccc aaggcagacc
     1441 gtttcgtcgt caagcccgac acggactata tttacatgac atcgaacaat accatccatg
     1501 ggacggaatg gcatgatttc ccgtctttcg acgttccctt gttctgtgat atgtccagcg
     1561 atttcctcag ccgtccggtc gatgtgaaga atttcgattt catctatgcc ggtgtccaga
     1621 agaatgtcgg cccggccggc gtcgtcgtgg ccatcatcaa ggacgacttg ctgaagaagg
     1681 tctgccgtga tttgccgctc atgctgcagt acaagaccta tgtcgaccac gattcgacgt
     1741 acaatacgcc gccggtcttc aacatttatt tcgtcaataa agtggcccac tggctcgacg
     1801 ataacggcgg actggctgcc atgcatgaac gaaataagaa gaaagcggct atcgtctacg
     1861 gtgtcatcga cgacagcaac ggcttctacc gcggccatgc ccgcgtagat tcgcgcagcc
     1921 tcatgaatgt caccttcaac ctgccgacga aggaactgga agcccagttc gtcgctgaag
     1981 cggctgatca cgacctcatc ggtgtcaagg ggcaccgttc catcggcggc tgccgggcat
     2041 cgatttacaa tgccgttacc gaagaaggct gccaggctct ggccgatttc atgaaagcgt
     2101 tccagaaaaa acattaatgt attacatagg gggagaagaa aatgaagatt ttagtcagcg
     2161 atgacgtatc gatgaaaggc gtagatctct tacgcgaaaa tggatatgaa gtagatgtga
     2221 agaagaacct cgatgaacag ggcctcatcg actgcatcgg cgaatacgac ggcctcatta
     2281 cccgttccat gacccacgtc acggaaaagg tcatcgaagc ggctacgaaa ttgaaggtca
     2341 tcggccgtgc cggtgtcggc gtcgacagca tcgacttgaa gagtgcgacg gcacggggca
     2401 tcgtcgtcgt caatacgccg acgtccaaca ccctggccgc tacggaacat acctgcgcca
     2461 tgatcatggc cattacccgc cacatcccgc aggcccacaa ttcgctcatg gccggcaact
     2521 gggaccgcaa gacctttacg ggcatccagc tccagaacaa gaccatcggc atcatcggcg
     2581 tcggccgcat cggttcgcgc attgccaaac gcatgcaggc tatggaaatg aagaccatcg
     2641 gctatgaccc gtacatcccg gaagaacggt tcaaacagct cggcgtcgaa caagtcgact
     2701 tggatacgct cttcaagcgg tcggactaca tcaccatgca tacgccgctg accaaagaaa
     2761 cgcggggcct catcggcaag gaagaaatcg ccaagatgaa agacggcgtc cgggttatca
     2821 acgtatcccg cggggcggtc ctcgatatct atgcactggc tgaagccctc aagagcggcc
     2881 acgtcgccgg cgccgctatc gacgtcttcc cgacggaacc gctgaccaag gacatcaacc
     2941 ccttcctggg catggataac gtcgtcatta cgccgcacct cggcgcttcg actgtcgaag
     3001 ctcagatcgg cgtttccgtc gacgtcgccg aaggcgtcat ggaagccctg aaaggcgaac
     3061 ccgtcgctac agccgtcaac atggctcctg taccgcccaa tgtctacaat gtcatccatc
     3121 cttatttcga cctcatggaa cgcatgggca acctggccgt ttacctggcc gatgcgccga
     3181 tgaaggaaat cgaagtcgaa tacacgggcg aactggccga tacggaaacg cgcctgctga
     3241 cgacggccgt cctcaaagga gccctcaatc cgatcctcca ggaatcggtc aactacgtca
     3301 atgctgccga agtcgccaag acgcgccaca ttgccatcaa agaaatcaac agccagtcca
     3361 atggctattt cgtcaactcc gtcactgtcc gggtccgcac caataagggc gaacacgcca
     3421 tcgtcggcgt cttgttcaac gggacggaag ccaaaatcgt ccagatcgat gaataccgcg
     3481 tcgacttcac gcccgaaggc tacctgctcc tggtaccgca cgtcgaccag ccgaacatga
     3541 tcggtcagat ttcgaccatc ctcggccagg ctcatatcaa catcatgggc atgcaggtcg
     3601 gccgcatgac caaatcgggc aacaacatca tggccattgc cgtccaggat gacatcccca
     3661 acgacatcat ggtcaagctc cgctcgatca acggcatcct cgatatgaag ctcattcact
     3721 gcgatattca ctaacagggg gaaactatgg tacgcatcat actcattcgc cacggcgaaa
     3781 cacaatggaa tattgaaggc cgttaccagg gccaggaaga cacgcacctg agtgaacggg
     3841 gcctccgcca gggccatatg gtcgccgaag gcctgaagga tacgcccatc gacgtagcca
     3901 tttccagccc cctcgaacgg tcgtacatga cctgctcttt ctgtgcggac ctccacggcc
     3961 tgccggtcct caaggacgac aggctcctgg aaatcaacca cggcgattgg gaagggaagc
     4021 tggccgatga aatcgaagcc aagtatcctg aagaattcca ccgctggcat acacagcctc
     4081 atacggtgac catgcccggc cagggcggcg aatccctgga agatgtccgc gtccgcgccc
     4141 gcgctgcctt tgatgattac gtccggaagt acgacggcaa gacggtcctc gtcgccgctc
     4201 atgacgccgt caacaaggcc atcatctgcg atgtcctggg cctggatatg agccatttct
     4261 ggcagattaa acaggataat acgtgtatca acgtcttgga atgtcaggac ggcgtatggc
     4321 gcctggtcct gctcaattct acgaatcata tggggtattt gttcagcggt attgaacaga
     4381 aaggcttata aggatataat acgaggggta tcaaagcata tttactgagg aaggaagaaa
     4441 ctatcatgtt agatacgaaa ttcatccgcg aacacgtgga agaagtcaaa gaaaatatta
     4501 agaaccgcca tgccaaagcc gatgtcgacg ctttcgtcgc cttggacaac cagcgccgcg
     4561 acattcttca gaaagtcgaa gccatgaaga acgaacgcaa tacggtcacc aaggaaatca
     4621 gccagctcaa acgcaacaaa gaaaatgcgg acgcccagat cgcagccatg aagaagctcg
     4681 gcgacgacat cgacgtcctc gacaaagaag tcaagaaagt tgaagctgac atgcgcgcta
     4741 tccagctcat gatccccaat atgtgccacc cctcggtccc cgttggcaac gacgacagcg
     4801 acaacccgga agtccgcaaa tggggcgaac cgacgaaatt cgatttccag cccctcaatc
     4861 attgggatgt cggtgaaaaa ctgggtatcc tcgattccga acgggctgct aaagtcacgg
     4921 gtgcccgctt ctattattac atcggcgccg gcgcccgcct ggaacgcgct gtctacaact
     4981 tcatgctcga ccagcataca gaaaaggacg gctacaccga agtcatcccg ccgtacatcg
     5041 tcaaccgcga ttccatgacc ggtacaggcc agctgccgaa gttctacgaa gatatgtaca
     5101 agctcaacgt cgaaggcgaa gaaatgtaca tgatcccgac ggcagaagta ccgctgacca
     5161 actactaccg cggtgaaatc atcgacggtg ccaagctgcc ggtctacttc acggccatga
     5221 cgccgtgctt ccgtgccgaa gccggttcgg ctggccgcga tacgcggggc ctcatccgtc
     5281 agcaccagtt ccacaaagtt gaaatggtca aatacgtcgc tccggaaacg agctatgatg
     5341 aactcgataa actgaccaac aacgccgaag atatcctcaa ggccctgggc ctgccgtacc
     5401 acgtcgtctg cctctgcacg ggcgatatcg gcttctccgc tgccaagacc ttcgacgtcg
     5461 aagtctggtt cccggcacag gggaaatacc gtgaaatctc gtcgtgctcc aatacggaag
     5521 atttccaggc acgccgggcc aatatccgct tccgccgcac gccgaaatcg aaaccggaat
     5581 tcgtccatac cctgaatggc tccggcctgg ctgtcggccg taccgtcgcc gctatcctgg
     5641 aaaactacca gcaggctgac ggttccgtca tcgtaccgga agtcctccgc ccgtacatgc
     5701 attgcgacgt gatcacgaaa cagtagtagg ggacgcccaa agggcgtccc gcagttcgca
     5761 ttcacaggcc gccggtcgcg ttcgtagggg gctgtcgcac gaaaaatttt gccatcatac
     5821 acggtccctt ttactttata gtttgtagta cagagtttgt agaagctggc tttaaatttt
     5881 ttaccatttg ctgcaaacta aaaactgcaa acttcaaact cgaaaaaggc gttcgcctgc
     5941 gataaacgat gaacgaaaaa actcctgccc cgaggcagga gtttttttac tctgtctaga
     6001 agtaatacta tatatgtatt tcaattattt ttagatagga ggttcactat ggatcgcgta
     6061 ttgaccttgg aatttgtccg cgttaccgaa acggctgccg tcaaggctgg ccgcctcatg
     6121 gggaagggcg acaagatggg cgctgaccag ttggctgtcg atgggatgca ttcgatttta
     6181 tcgacggttc ctattgatgg gactgtcgtc atcggcgaag gcgaaatgga cgaagcgccg
     6241 atgctctata tcggtgaaaa agtcggcgcc ggcggcacgg aagtcgatat cgccgtcgac
     6301 ccgctggaag ggacgaacct gacggcgaaa ggccaggatg ggtccattgc cgtcatggcc
     6361 atcgcccgca aggggaactt gctccacgct ccggacatgt acatggaaaa gctctgtgtc
     6421 ggaccgcggg ccaaaggccg catcgacctg acccagcccg ttcaggaaaa cctgcgccgc
     6481 atcgccgaag gcctggaacg aggcatcgac gatctgaccg tcgtcatcct cgaccggccg
     6541 cgccatcagg aaatcatcga cgaatgccgc agtgccggcg cccgcatcaa gctcatcacg
     6601 gacggtgacg tctcgnnnnn nnnnngtcga cgccggtatc gaagggtccg gcgtccacgt
     6661 cctcatgggc atcggcggcg ctccggaagg cgtcctggct gcagcggccc tgaaatgcct
     6721 gggcggcgat atgcaggccc gcctcattcc ctataccgac gaagaacgga agcgctgctg
     6781 ggaaatgggc attgatgaca tcaaccgcgt catgaccctc gacgacctgg tcaaaggcga
     6841 cgactgcatc ttctcggcga cggccattac cgatacgccc atgatgcgag gcgtccagta
     6901 ctttggcggc ggcgcccgca ccatgtccgt cgtcatgcgc tacaagagcg gcacgatccg
     6961 cttcatcgac acgttccacc gcttcgacaa gaaggccaaa tggagtattc atttgtaaaa
     7021 gccgtagttc gcaggccgca gttcgcagtt cgcaggccgc tgtcagccgt cagctgttag
     7081 cagtcagcag tcagtcgtca gcagttagcg tcccgcttgt aggttgaaga aaaatttccg
     7141 agtaatttcg tctgtagggg cttgcacgtg gcaagcccgc tccggaatat gagtgattnn
     7201 nnnnnnnntg ccttttggcc atttagaata aactactcga atagtattag gaggcaccag
     7261 aattggagaa acttgttatt catggtggaa agcccttaca gggtacggtt cgcgtcagcg
     7321 gggctaaaaa tgccgtgctg cctatcattg tcgcatctat gctgggaacg acgcagagta
     7381 ctttgacaga aatcccgaaa ttagccgacg tacataccgt ctgtgacgtc atcgcgtccc
     7441 tcggcgttca tatagaacat cctgaaagag atacactggt catcgatgcc catgagctga
     7501 catcgacaac ggctccgtat gatttagtgc gccgtatgcg tgcatctttt ttggttatgg
     7561 gacctttact ggcccgcaag gggagggccc agatttccct gcccggcggc tgctccatcg
     7621 gtgcccgtcc tatcgacctg cacctcaagg cttttgaagc catgggagcc gtcatcaacc
     7681 tggaaaacgg agacatcgaa gcgacggcac cgaatggcct caaaggggct cagatctatc
     7741 tcgatttccc cagcgtcggc gctacggaaa atatcctcat ggctgcatcc atggccgacg
     7801 gtaagacggt cctggaaaat gccgcagaag aaccggaaat cgtcgatttg gcaacgtacc
     7861 tcaacagtat gggcgccaac atccgcggtg ccggcaccaa cgtcatccgc atcgaaggcg
     7921 tcaaggaact gcatggcgcc aatcacgccg tcatccccga ccgcatcgaa gcgggtactt
     7981 tcatggtcgg cgcggccatg acccagggca atgtcttcgt agagaatgcc atttcggaac
     8041 acttgaagcc cctcatttcc aaattgaaag aagtcggcgc cgaagtccag gaagaaatcg
     8101 acggcatccg cgtcatcggc catgaaccgc ttcgcccggc cgatatcaag accctgcctt
     8161 atcccggttt cccgacagac atgcaggccc agttcatggc cctgacgact atctgccagg
     8221 ggacgagtgt cgtcaccgaa acggtctttg aaaaccgctt catgcacgtc gacgaattca
     8281 agcgcatggg cgccaagatc cgcatcgaag gccgcagcgc catcgtcgaa ggcgtcccca
     8341 ggctcaaagg ggcctctgtc aatgcgacgg acctgcgggc cggtgctgcc ttagtcctgg
     8401 ctggcctcgt cgccgaaggg ggaacggaag tcggctattt ataccacatc gaccgcggtt
     8461 atgataatct cgtattaaaa ttacaaaggc taggtgcaga cattgttcgc gtcaatattg
     8521 aggaagaaga aaactaagaa aagagtacag ccgaaacagt cccactcgtt ctttctggcc
     8581 aactggttcg gcagcgatat cgctatcgac ctgggaacgg ccaatatcat cgtgttcgtc
     8641 aagaaccggg gcattgtcgt caatgaaccg gccgtcatcg ccgtcgatac gatggaaaat
     8701 aaagtcgtcg ccatcggccg cgaagcccag gctatgctgg ggcggacgcc gcgtaacatc
     8761 cacgcctacc atccgctgcg caacggcgtc atcgccgact acgacgcgac ggaatacatg
     8821 ctgcgctact tcatccgcaa ggccatcggc aaggcactga tctttaaacc ccgcgtcgtc
     8881 atctgcgtcc cgtcgagcgt caccaacgtc gaacggcggg ccgtcatcga agcggccatg
     8941 caggccggtg cccgcaagac gacggtcatg gaagaaccgc tggctgccgc tatcggcgcc
     9001 ggtctggaca ttgccacgtc taatggttcc atggtcgtcg acatgggcgg cgggacgacg
     9061 gacgtcgccg tcctcagcct gagcggcgtc gtcatcagcg aatccctgcg cattggcagc
     9121 aacacttttg acgaagcgat tatccgctat ttggaaaaga tcaagcacgt cgccatcggt
     9181 cagcacacag cagaagagct gaagatactc atcggcacgg ccatgggcga cggccggcgc
     9241 atgacagccg atgtccgcgg ccgcagcacg gaaacgggcc tgccgacgtc ggtcgatatc
     9301 gactcgcagg aaatccgcca ggccctggac gaaccgattc agaccgtcct caagacgatc
     9361 gtctccatcc tggaaaagac accgcccgaa ctggctgcgg acattgccga ccatggcatc
     9421 gtccttaccg gcggcggcct gctcctggat agtttcgacc gcctcatttc ccagacgaca
     9481 ggcatggccg cctatctctg cgatgaacct ttgttgtgcg tcgccaacgg agccggtaag
     9541 gctttgcggg aaatggaccg cttaaaagat agttttgatg atttatagag tgctccgcag
     9601 caggcggagc tgagaaggag ggagccgtca tgaaaaagtt tcgcagactc gccgtattag
     9661 ctgggttagc aggtctgatg gctatgccgg ccgcagctgc tgacgtatcg tacacgacgg
     9721 tattgagcgg cagcgtcgtc agcgtagccc ctgtcggtgc agccgtcaaa gagggagatg
     9781 tcctgcttac ggtgaattcc ctggccggtc cgatgccggc tgcccgttct gacaaggatg
     9841 gcgtcgtcaa gaccgtcctc gttcagccgg gatcacaagt gcagcaaggc gccgttgtcg
     9901 tcgtggtgga aacgaaatga gatataccat gatgaagaaa gtctccgcct tgcggcgtac
     9961 gttgggccgg gtctgtctga ccgcggccct ttcctgtatc ctggctcttt cggcacaggc
    10021 acaggagttc atgaatgtca gtgaagtcca gccggggatg acgggctatg ccaagaccgt
    10081 cgtccacggt acgactattg ataccttccc cgtcgaaatc ttaggggtca tgaagaacag
    10141 cggcccgtca ggggacttgg tcctggccaa gttttccggg cccctcattg aagaaacggg
    10201 tggcatcgct cagggcatga gcggcagccc ggtctatatc aatggcaaac tgctcggtgc
    10261 cgtcgcttac ggctggtcct ttaccaattc ccgcgtcggc atgattacgc ctatcaacga
    10321 tatgatcaag ctctggaatg tgccgacgag agaagaaatc cggcctttca acgcccgcga
    10381 atcatcgctc atccccattg cgacgccgct catgacgtcg ggcttcgaca gcgtgtcgac
    10441 agcgtggatg cagagcaaac tgccgggcta taacttcatg cctgtcgata cggcttcggc
    10501 cagcagcgac agtacggccc tgcccctgga accgggcagc agcgtcgccg cagccttcgt
    10561 caacggcgat atgaagatgg gtgccatcgg gacggtcact tacgtcgaca acgaccagat
    10621 cgtcgccttt ggccatccct tcctgaaaaa gggcagcatc aattatttca tgcacaatgc
    10681 ctacatcttt acggtcgtca ataacctgtc cagttccttt aagctgggct ctatcggtgc
    10741 cgaagtgggc aagatcactc aggaccgcgg cgccggcatc ggcggccagt atggctatct
    10801 ggcaccgggc atccccgtcg tcatcaaggc cagggatacg gacaccgaca cggccatgac
    10861 caagcgcgtc aagatcatcg aagacgatga actgacgccg gtcctggcgg ctacgacggt
    10921 gtacaatacg gtcaataaga ccatcgaccg cacgggcggc ggcacggcga ccttgtcgta
    10981 taccatccgc tcttccaatg gccgtgataa agatattacc cggcacaata tgtattattc
    11041 cgaagataac atcaatgaaa agagtatcga cgagctctac aatgtactgg atatcttgaa
    11101 acacaatgag ttcatcgact atcctatcct ggacatcaac atgtccgtcg atatcacgca
    11161 ggcctgcaag tcggcgacca tcgtcgatgc gtcggcagcg cctgtcgtcg tcagcccggg
    11221 cgatacggtc tatttcaagg tcaagctcca cccgtaccgc ggtgtcgatg aagtgaagac
    11281 catgagcttc accgtcccca agaaccagcc tctcggcgat atggtcctgg aagtgcgcgg
    11341 cggcggcgtc attcctctgc cgtacctcat cgaaaaacag cgctacaacc tgactgacga
    11401 aatcgtccag cgcctgcgcc attataagga tttcaatgaa ctgaagaagg aaatcgaaaa
    11461 agaagatgcc aacaatgact tggtcatcga aatcctcagc cagggcgtca gcatgatcaa
    11521 cgaaagcggc acgaaagtga agaaggaaaa gatccgcggc aaagaaccgg ggaccagcgc
    11581 caaggatgtc atcaaaccgg gcaagcacaa catcggcgat gaaaatccga acgacgccaa
    11641 atcttccatt ccgacaccgt atatcgtcaa aggcgacggt cagatcacca tcaaagtcgt
    11701 cacgccggaa aaacgcgacg cttatttggc caagaaccac aacatcaacg aagcgaaagc
    11761 cgtcatcggt gaagaaaccg acgggtcctc gcaggatgat gaattggtca aagccgataa
    11821 gagcagcgat gacaacaagg atacaaccaa gaaagatgac aagggaagcg acaagaaacc
    11881 cgataaaggc caggataaga aatcggctga accgatccgc accgtcgacc tggatatgtt
    11941 ccactagccg ttcgtagggg acgcccaaag ggcgtcccgc aggccgtagg ccgcatgtcg
    12001 cagtcagcag ctagccacta gccactaacc acgagccacg agccactagc cactaaacag
    12061 gcgctgtctt catatgacag cgccttttcc cttgccattt ttcgcataaa atgctattat
    12121 tttagctgta gaaaactcaa attgcaattt tagttttcct tattttcttt ctcacaattt
    12181 aggcacaatt gaatacaggt gttttacatc catttttaat gatggtatag taaagtagta
    12241 tgatacttaa agaaaggacg gtctttcatg caggaaattt tggaaggcat cggcagcttt
    12301 acgctccgtg tctttgaaca agtcggcgtc attgccatcc tctttggcca gaccctgcgt
    12361 cagctgccaa agatacaacg ccgcctgacc cttgcccaga tggctcatct cggcgttaat
    12421 tccctgccta tcgtcagcct gaccctgctc tttgccggca tggtcatgac cttgcagatc
    12481 gtcgatattc tcttgcgcta tggcgcccag tcgacgctgg gcggcgtcat gtccgtcgcc
    12541 atgggccgtg aattaggccc tattttaact ggcgtcgtca tggcaggccg cgtcggtgca
    12601 gccatgacgg ctgaaatcgg gaccatgaag gtcacggaac agatcgatgc cctgcgctgt
    12661 atggccgtca atcccattgc ctatctcgtc gtaccgcgct ttgtagcctg cgtcgtcatg
    12721 gtgcctatcc tggcctttta cggctatgtc atcggtacgg ccggcggcta cggcgtggcc
    12781 acattgggcg ccgggctcac gcatttcacc tatgtgaatt ccatcgaact ctttacggaa
    12841 gtcagcgacg tcgtctacgg cctcatcaag gccatgttct tcggcggtat catcgccctc
    12901 gtcgcctgca atgaaggcat gcatgcccag agcggggccg aaggcgtcgg ccaggctacg
    12961 acgaagtccg tcgtcacgtc gattattttc atctttatct gcaattatat cttatcggtt
    13021 ctattgtatt gaggagtgct atgatccgtc ttcgcaatgt gaccgtcgcc tatggcgacc
    13081 gggtcatact agacaacatc aatttagata tccatcaggg tgaaaccctg gccatcctcg
    13141 gtgccagcgg ttcaggcaag agtaccatcc tgcgcctgat catcggcctg cagaagccga
    13201 cgaaagggca ggtcttcttt cacgatgaag acattactac ctttgatgag gaacagctga
    13261 cggctgtgcg ccgccacatg ggcatggtct tccagtattc ggccctcttc gattccatga
    13321 ctgtcggcga gaacgtcgcc tttggcctgc gccagcatac gaaagacgat gaagcgacga
    13381 tccagcgcat cgttgaggag aaactccacc ttgtcggcct ggaaggcatc gaggacatga
    13441 tgcccaacga tttatcaggc ggcatgaaga aacgcgtcag cctggccagg gccattgccc
    13501 tggacccgca ggtcatcctc tacgatgaac cgacagccgg cctggacccg ctgcgctgta
    13561 tggatatcaa ccgcctggtc gtcagtatgc agaagcagat ccatgcgacg tcagtcatcg
    13621 tcactcacga tatggactcg gccttctatg tcgctgaccg cctggcttat ttacaagacg
    13681 ggcgcttccg gctcattgcc gataagaaga cctttgccca tacagatgat gaggaagtgc
    13741 agcgctttat ccacggcggg cgtcttccag aagaaagagg ggatagccta tgaagtggag
    13801 tgcagaagca aaagtgggat tggtcactat cgtcggcgtc ctcttattta cctttgtcgt
    13861 cctgaccctg gctcatgccg aaatctttgg taagcccggc tttgtcgtcc atacggaatt
    13921 caaggatgcc aatggcctgc aaaaagggaa ttccgtccgc tatgtcggcg tccacgtcgg
    13981 taaggttgaa agtgtaaaac cgtcccgcga tggcgtcgat gtggccgtga aactggaaaa
    14041 agggacggaa atcccccgcg attccaaagt gacgattacg acggacggcc tgctcggcga
    14101 aaaaatcgtg gccattacac cggggaatga caagaatcat cttctcgctg atggagatta
    14161 catcgatggc acacagagca agaacatgga tgacatgatg gatagtgccg gcaagctcat
    14221 gggcagtgct gacgatatgg tcaagaatat caatgccatc atcggcgatg aaaagacgca
    14281 ggccgctatg cgcggttcca tccagcatat cgacgatatt accggcaaga cgagccagat
    14341 gatggatgcc aatgccggca atatccagca gattacggct aatatggcag ccatgacagc
    14401 tcagatgaac gcgtccatgc agaacctcga cggcgatggc gcggcttcag ctaacgtccg
    14461 ggccacggcg gccaacatga agcagatcac cgaccgcttc gagatcattg cccagtcgat
    14521 ggaaaagatg acgacggatc cgcagaccca ggccaatatt cagacgacgc tgaataatac
    14581 ggcccagatt acaacaaaag tgaataagat actcggcggc gcatcggaca tcaaagtcca
    14641 gggcgaagcc ggactcttat ataatacgac gaagaatgag accagcggca ctgtaaattt
    14701 taaggtgtac cgtggcgata cgttcggcct cgtcggggca gagaacatcg gcaacggtac
    14761 gaacctcaac ttgcagtacg gccgccgcgg ccgtctgttc gacacgcggg ccggactgat
    14821 caacggcgac ctcggtgccg gcttggactt cttccgggac gggcctttcc gcttgtccct
    14881 ggaaggctat gatcccgatg actggcgctt ccgtttgaaa gcccagtacc gcctgacgcc
    14941 ggatctctat ctcttcagcc agttcacgcg gcccatgagt cgcagtgacg gtggcaatta
    15001 ttatggtatt agttacgctt tttagcagat tatagaaata aacgggggta cccatgatga
    15061 acaacaaact gtacaagaaa ttggtcatcg ccatgatgat tacgaccata agcgcagtcg
    15121 ccgccgcttc ggcagcggat acgacggctt ctgtccgtcc tgtcgactta gcgcaggccg
    15181 atgcgatcca gtcccgcgcc aaggcggaac agcaggaaat caaggctgaa aaagctcagc
    15241 agaagaaagc cgccaaggcc gaagccaaag ccaagaaggc tgccgccgcc gtccggcccg
    15301 tagacattac gccggaactg cgcgaagaac tggctcagaa aatcgctgac gatgccgcca
    15361 agagtgctga aaaagcagcg gataagcggg cgaagaacca ggaagtcgac ccggtcatcg
    15421 tcgtcggccg cgtaccgacc aatggcacgg tcgatttgaa cctgcccaag actgttcaga
    15481 tggccctcga ttataatcgg gatatcaaga attcccagta tgccctgaag aaggctgaat
    15541 acaccattaa cgaagccaaa gccggcaagg cgcctgttgt cgattatacc ttcaatacac
    15601 agcgttctcg tgcaaccgat gccagcctgg ctgcttcccg cagcaaggat gccatctcca
    15661 atgcttttgg caataattta tctgtcacca ttccgctcta tacgggtggc aaagtcgaag
    15721 gcaatatcga cgtggctaaa ttaggcaaga ccaatgccca ggaagacatc cttcgcgtcg
    15781 aacaggcgac gaaatattcg gctatccagg gctattatgg cctcctggct tatcaggaac
    15841 tgcaaggcgt ctaccatgaa gccgtcgaca acctccaggg ccacttagat aacgtccagg
    15901 ctcagtacaa tgtcggcacg aaggcacgag tcgacgtcct ctcgtccgat gtctccctgg
    15961 ccaacgccaa gacgacggcc attacggcag ataacaacgt cgccgttgcc gaatcgaact
    16021 tgaacaacat cctcggcctg cctttggaaa cgaagctcaa cctggccgac catcagctgc
    16081 ccttcgatac gtacaacatt tccctccagg aagcgacgga ctatgccatg aaatatcggc
    16141 cggaagtcct ccaggcagcc attgccgtcc agcaggcaga acgtaacatc gacatcgcca
    16201 atgccggcaa taggccgact gtcgccatta caggcggcaa taactgggcc gacaacacct
    16261 tcccaggcat cgatgccaat aaacgcagct ggaacatcgg tgctggcgtc acttataact
    16321 tctatgatgg cggcgcgacg aaggctaaag tcaaccaggc caaacaggat ctcctcgtgg
    16381 cccgtgaaac ggaacagaag acccgcgaag ccgtccagct ggaagtcaag caggcctacc
    16441 tcaacatccg cagtgccgcc cagaaggtcg aagaaacgca gaccgtcgtc gaccaggccc
    16501 gtgaaaacta tcgtatccag aatatccgtt accaggccgg cgtcggcatc aacctggacg
    16561 tcctcgatgc ccagttgagc ctcaacgaag cccaggtcaa ccatatccag gccctgtacg
    16621 actacaacgt cggcattgcc aaactggaac aggtcatggg cgtcgatgtc gaatcgggtg
    16681 tcatccatcc gtctttgacg gcgacgacat atcggggata aacagggctt aaagagatga
    16741 tgacaggttt ttaacaattt acaagggaaa tatcctattt gtcatctatt tcttgatttt
    16801 cctcaactcc ataggtaaaa taaagagaga attgtaacgt gcaattctct ctttttttgt
    16861 atgcggagat cgattatgaa gaaaaaatgg atggctgcct gtggcgtcac ggcctttttc
    16921 ctgtcagcag ggattgccct gtggnnnnnn nnnngtggat ggaaaaaccg gctctgaccc
    16981 agatggctgg ccaggcgatt acgacaactg caaacagtaa attgaacggg accttgtcgt
    17041 tttcttcact ggatatatcc ctgaacggcc agctggtcat ggccaagcca gtcattaaag
    17101 atgcacaagg gcgggtcgtc atcgaaggcg atgccgtccg ggtttatgtc aaccccggca
    17161 agatcatgac ggctctaagg cagggcgaaa tcctgcaggc cctggatacg gccgatgtcg
    17221 acgaccccgt cctgcatctt tggcagaatg aagacgatgg cacgtggaat gtggcgtcgc
    17281 tcatcaagaa gaatcagagt acgaccgatg ccggtttccg cggggccatc aatgttcaca
    17341 acgggactat cgacgcccaa ttaccggaca gcacggtcgt caccggagaa aatgtcaacg
    17401 gcagcgtggc ctttgccgac tatccgtcgc tggccatcga tgcgtccatg accgtcgatg
    17461 ggcaaaaggt gacggctcac ggaaggtatg cctcgaaccg ccagtatgat ttcaccgtca
    17521 gcgccgacgc cgtcaacggg acttatgcgt cgtcattcat cccgtcgtcg gccgatgtcg
    17581 tcatccgcag cgggaccgtc gaaaacgtca aagtacgcgt cgcggacagc cacaacggct
    17641 ttttcctgtc cggccaggcc gacgtatctg acggctacgt caccgtccag ggcctcgagg
    17701 tagacggcct gaaaggccat gcggccctga cgagcaaaga tattacctta tcggacgtcg
    17761 aaggccgtgt caacggccag gacttccgcg tcggcgggac tattgtaacc aacggcgaca
    17821 cgccggtatt caacctgaac gtcgacgttc agggagccga cgtgacggcc tttgccgatt
    17881 atctgcccgt tgctatcagc gggacagccg gtttcaaagg gacggtctgg gggacggcac
    17941 aggacgtgaa tgcccgcggt acagcgtctc tgcacgatat gacttataac ggctataccg
    18001 tcgataaagc ccaggcagat ttgatttaca gtcagaaccg gctcgatatc gattcactga
    18061 cggcccaggc ctatggcgct tccctgagcg ggaagggcgt ctatgacatc cagagcggtg
    18121 cctatgaagc cgatgccgac gtagacggac tggacctctc ggccgtgccc ggcgtgcctg
    18181 tggccgtcat ggggagtctg tcggcgtcgc tccacgcagc cggcaacagt aaggataact
    18241 cgattatggc gacaggccac gtgacggcag gggacctgtc ttataatggc ctgactgtcg
    18301 actcggcagc cggcgatgtg gcctatgacg gccgcgtcat gaccttgcgc agcggtcatg
    18361 ctgacctggc aggaggaagt atcgacgtca gcggttctta tgaagtggac agccagacgc
    18421 cgcacctgtc ctttaccggc catgacctgc ccctcgacgc cgcttcgcct ttcgtgtccc
    18481 ttcccatgga tgggacggcc gatttgtccg gccacatcga cgggcagcag tgggatgtgg
    18541 cctttaatgc ccgcagcggc cagatcaagg gcgtcccctt cgacagcatc gacgggacgg
    18601 cccggggaca gggcagccgc atcgaaatcc cggccctcta ctggcgccgc ggtgatggga
    18661 cccacaccct gcacggccag gcggacctgg atgcccggac gatacaggca tcgctgacga
    18721 cggcgcatat gcgcatcgaa cagctcctgc cggctatcgg caaggcagac ctgccgctca
    18781 ctggctgggc cgataatacg attaccctca gcggcagcct ggataatccg acggcttccg
    18841 gctcgttccg cctgaccagc ggatcctatg caggctacct ctataagaat gtcagcgccg
    18901 attaccgact ggataatggg acagtctatc tctcgaatgg cgatatttct tcttacacgg
    18961 catccctggc cctgtccggt tccatcggcg atacgctcaa tctcgatttg atcgggaaga
    19021 acgtcgatat ttcccacctg gtaccgcaga acaagacgcc gcgcagcgga tctttcgacg
    19081 tccaggccca tatcggcggc agcctggaca atccgacggc agccggttca ctgacggcgg
    19141 ccaatctggt catcaaccac atggccctga acaatatcca cggcgatttt gcctattatg
    19201 acaatatgct gcgcctgacg gacctccatt tcgcccagct cggcgggacg tatgacggca
    19261 atttattgta taatacgaaa tcgtctctcc tgcggggcca ggcgacggtc gtcaacggcg
    19321 acattgccgg tctgctcaag gtcgcggccc tgccagtaca ggatattgcc ggcaagctga
    19381 atggccaggt cgacatcagc ggcacaggtg acaacccgac ggtctccatg aaggggacca
    19441 tcagtgacac ttctttcggc ggccaggccg tcgaaccggc tgacatcgac gtccagatgg
    19501 aaaatagcgt cgtcaccatc aacaagatgg ccctgaacat cggcagcggc gtcctggctg
    19561 cccaggggac gtatgccctg cacggtccgg tgaaattgtc cgtcgctgcc aagcagttcc
    19621 cggccaaggc cctgaccgac gttctgggac ataaagactt cgtcgtcgat gcgcctatcg
    19681 actttgccgc tgacctgagc ggtacaggcg atgacctcca ggccgacgta tcggcccagc
    19741 tgggaagcgg cacggccaac ggcatttcct ttacgggcgc ttatgccctg ttcaacatcc
    19801 gcaacggcct gattactttg cagcaggcca gcggcagccg cgacccctat aaggtcagcg
    19861 cacacggtac cattcccgtc agcgccttgt ctggcggcaa gaccggcgaa tccatggacc
    19921 tttcgctgca gctcgataac gccggcctcg atgtcctgac tttcctgacg ccttacgtca
    19981 ccgaagccga cggtcccatc cagggggccg tcaaaatcgg cggaaccctg gatgcaccga
    20041 cggtcaacgg cgatattgcc gtcaagaacg gcaccatcaa gttcaaggac acggcttatc
    20101 ccctggccaa catcaatgct gacctggcct tcaaaggccg ttcggccgtc ctcagcggca
    20161 gcggtactat ggataagaaa gggaagaaga atcccggcag catcagcctt gacggcaaag
    20221 ccgcctggag tggccgcagc ttagattcgt attccctggc ggctgatttt tccggcctct
    20281 atctgaactg cccctattac gacggtccca tcacgggcta tgtcaacgtc acgcctggcg
    20341 acgggcggcc taaagtgagc ggcctcatgc aggtcgacaa tacgactgtc gacattcccc
    20401 tggctctggc cagcagcagt gaagggcccg atttggacct ggatttcacc ctgaccctgg
    20461 gcaacaaggt ccgtctgtac aatctggctc tgtacgactt gatggtcaat ggatcggtca
    20521 ccttccaggg caacctcaat catccccatc cgtcgggccg ctttgaagcg acgcggggga
    20581 ccgtccatta tttggacacc aatttccgcg tgaccaaggc taaggccgat ttctcacggt
    20641 atgattcctt cctgccctat gtcgacgctg aaggcttcag ccgcgtcggc cagtacaatg
    20701 tcatgctgac cctgcgcggt caggcggaca acatggacct catgctccgc tccgacccgc
    20761 ccctgacgaa gcagcagatc gtatcgctca ttaccctgcg caacagcggc ggccgccccc
    20821 agagctccct cagcgaggaa gatatggaca gcctcctcgg atcgggcatc cgcatgaccc
    20881 tgaacagcct gggccttaca cagagcctgg aaaaggcctt gtctctggat atgctcaccg
    20941 tcaccaacgg ctcgctgaac ctcaatgata agaataccga tgtgggccgc aactattaca
    21001 acattgaaat ggggaaatac ctgttcaacg acttcatggt cacggcggcc ttcggcatca
    21061 accacggcga cgaccgcttc ggcttcatgt atgacctggg cagcaagttc agcgtcaacg
    21121 cctggacgtc cgacgacgac agtttcatcg gcggtgtcta taagtattcg ttttgattcc
    21181 gtatatgggg tcatgccgtt gcatccgggg attgatggca ggacgccata catacaggaa
    21241 taacgggaag gaaacatgtc ctgaagcggg cttgccacgt gcaagcccct acagatggga
    21301 cacaggccca tccctgtata tgtgtggata cgccattgcc tttgtagggg cgcccgtccc
    21361 tatatatgtg tgaattccat ttgaatgaaa aggtggtgaa aggccatgtt agacgattat
    21421 ctgaaagaac tacagcacat caccctgctc cagcccgacg aagaacgggc cctgtggcag
    21481 gcctataagg acgaggggaa tctggaggcc cgcagccgcc tcatcgagca gtaccagccc
    21541 ttggtcttca aagaggccat gcgctggcac atccatcgcg acatcctgtc cgacgccctg
    21601 caggaaggga cactgggcct catcgaagcc gtggaacgct atgattaccg ccgtggcgtg
    21661 gccttcccgc tctttgccgt ccaccgcatc cgcggggcca tcctggacta tctgaagcgc
    21721 gaaggcgccg tcagcgccgt atcccttgac gaaccggatg aacggggcgt gacgttgcag
    21781 gatacgctct gcgatgagcg ccaggaccct gtcgaagcca cgagccgcca gctgttgctg
    21841 gaaaaggtcg gccacgtctt gaaaaggctg cccgaaaagg aacaggccgt cgtcgaaggc
    21901 gtgtacctga acgacgtgga gcagaagcac ctggccaaga gcctggacgt cagcctgcct
    21961 tacgtctacc gcctgcagaa acggggcatc cgccgcgtgc gcggcatgct cagccggttc
    22021 atccatgaga gccgccacta aaattttttt acattttctt aaaattgacc cgtttttagg
    22081 tgaaaataat cgaaaaatgc tgtaaaatat agattatggc atgtttttgc gcctttttgc
    22141 cggtataaat tcatacggcc tttgccgatt ttataagagt attcgtctag gcagaaagga
    22201 gaaggctgta tgaaacattc ccaacgcgat acagttcacc gggtccgggc cgtccggtcc
    22261 tggctggaaa aggccgaagc ctcgtttgat gaagagtccg atatcaaggg cgaactcaat
    22321 ctgatgctgg ccgaagcgga aatgaagaat ttgcgcaaac accatccagt cagcaggaac
    22381 tggctgcggg ccggtgccat ggtcacgtcc ttcttcctgg cgctgggcgg ctggtatgcc
    22441 ttccgcctga ccgggccgga agaacggcca ccggccgtcg aggccgtcgt ccaggagacg
    22501 gcggcaggga atgaagcgga ccgggacccg gtggatacgg ctgcgcctgt ggccgagccg
    22561 gtcccggcag gcagcgtgca gccccctgtc caggaaggac cagcaccggt ggcagcatct
    22621 gcgccgccag cagctcctgc cggagacgaa ccgcctgtgc agccagccgc gacggctgca
    22681 gccgtaccgg aaccgccagc agcggctccg gtacgggtgg caaggaaggc agtcttgtcg
    22741 gaccgccaga tacaagctac tgtacaagaa gcacgtcata ccttgcgcgg tacaggtatt
    22801 acgaaataga taatggagga tttttatgaa tatgcggaag aaaaaacttc tcgccctgat
    22861 ggtaaccagt gcgatgatga cgtgccctgt ttgggctgat gatacgacag cggcaacagg
    22921 ttatgagtcg tcgggcagcc agacggttac atctggctcg gcagctgctc cggcggatga
    22981 ccagaaaaca gctgtcaata cggtcgtaac ctcgcccaat gatgaaacga cagccgatgc
    23041 catgaaagcg gctaaatcgg gcctgacggg ctatgaaacg gcagaagaac tggccgccat
    23101 tgcggctggt aagagtccgg ctgcagttca gcaggaaaca gcagaagccg tccacaagga
    23161 aaatgaaatc ctcaacccgg ctcctgtcga tacgacgccg cagattacgg gcgacaaagt
    23221 cagctacatg agcggtacca gcgacatgca gtttgatacg tccatccccg gctatcccct
    23281 ggccgtcatc caggccggcc aggacgtggc cctgccttat ggggaagccg ttaccgatgc
    23341 cgacttgaaa ccctatgtcg acaaggtagc cacttccgtt acggtcggcc ccgtaccgga
    23401 agcccagatt gaacagaaac tcctaccgca gctggccatg cgcgtcggcg atgccgtcaa
    23461 catggactac atccgtcacg acctcaacgt catcggggcg acgggcctgt tctcgacggt
    23521 caagccgtcc tttacgactg taccggaagg cgtttccctg aactatacgg tccagatgaa
    23581 tccggtcgtc aagaaaatcg acatcgtcgg caacgaatcc ctcagtacag gtgatttgat
    23641 gaaactcgtc gccacgacgc cgggcacgac gctcaacacc gccgtcgtca gccacgacgt
    23701 agccaatatc aacacggcct ttgccaatgc cggttatatg atgagccgcg tctccgatgt
    23761 ccgcctcgac gacgaaggcg ttttgcacat tgccatttct gaaccgcgca tcgaaaatat
    23821 caatctccgc ggcaatacca agaccaagaa taaggtcatc atgcgtgaac tgcgcatgaa
    23881 gaagggcgat atcttcaaca agaacgctgc cagccgcagt atccaacgtg tttacaacac
    23941 gggctatttt gaagacgtca acgtccgcct cctgccgggc cagcgcaatc ccaaagacgt
    24001 catcgttgaa atcgacgtca ccgaacagaa gaccggttcg gtcaccatcg gtgccggtta
    24061 ctctgactcc gacggcctgg tcggcatcct cggcctggct gaaacgaacc tgcgtggtac
    24121 aggcgataag gccaatatca gctgggaatt cggcggcaac acggatagca acaagaacta
    24181 catcttctcg tatacccatc cgtacctcaa cgatgccggc gactccatcg gcttcagcat
    24241 cttcgaccgc gaatcggaat acgacgatta tgatgaaaag ggcaattccg tcgctgaata
    24301 tgaccgccgg accaatggct tcaacgtcac gtacggccgt gtccgcagcg aatacgtcag
    24361 cgactacgtc actttggaaa caaagcggac gaaatacacc gatcacgata gcgggtttaa
    24421 ctatagaaac gatgcaaaag accctgataa taaaggatat gattttgcgg gaatggatta
    24481 cttgggtaag aacttcggcc gcaccaatag cctgacctgg tcccacgtct tcgataaccg
    24541 cgacaacgtc tacgacccga cgaaaggcaa gcgcctgtcc tttacgggca cctgggctgg
    24601 ccacggcatg ggcggcgact tcgactactt caaattcatc gccgaaaacc gcctgtacta
    24661 taaagttggc cgggcccacg tcatcgccgt ccgcctcatg ggtggtatcg ccaccggtga
    24721 tatgccctac aacgacctgt tcaccctcgg cggtgccgac aacctgcgcg gctatgaaga
    24781 cgatgaattc cgcggcaaca agatgtacga agcgaccgtc gaataccgct atcccattgc
    24841 caagaagatc cagggcgtcg tctttaccga cgtgggcaat gcctggggtg gcgtagaaaa
    24901 catcccctgg taccatgaaa acaataaact ccattattcc ggtggtatcg gcttccgcat
    24961 caccacgccg atcgggccta tccgcctgga ttacggcgta ggccaggatg gcggcaagtt
    25021 ccacttcagc tttggcggta agttctaaga cgatgaaagg tcgtggatga tgaagatccg
    25081 cacttattta ttgactgtct ttgccgtctg tgctagtctt agtagtgcta cagcagcatc
    25141 ggtagaccaa gtgacggtcc atgtcgcctc cgtagaggag gcgattccgc cattagtcga
    25201 gaaacgcatt gctgccagca tccaaactgt agggaaccac atattcctca accacgacag
    25261 cggggaaatc gtcagccgtc atgaagaata cgagcgaacc gtcaacgata tcatcaaccg
    25321 cgtcctcata ggctatacgg tggaatccct tgccatccat cccggcagcc ggacggacct
    25381 ggatgtgcgc atccgcccct ggggcgatac gatccgcaaa gtccactttg ttatcgacta
    25441 cggcgctctg ccggcgatgg gccaggccct ggtcgataag gacctggaac acgccgggga
    25501 tatggctgag agcctgctca cgggactgcc cgtcgatgcc ctggattggg ctaacggcgc
    25561 cgtcaagtcg gtcctggaga atgaactgga agaccgggtg ccggaattct atccgcacat
    25621 cgtcatccac ggcgggcccg agacagacgt cacggtctat atgctgccca agctgcctgt
    25681 cgtgcgcaac gtccgcgtcg ccatcaaggg cgatgatgtg cccaaggtca tcttcctcag
    25741 cgcccgcaac catctggaac gggactacgc cggactggac ggcctgcctg tcgccttcgt
    25801 taagcgccat gagcgggaca tcgccgcagc cttgcaggaa gacgtccagc gtcagtgggt
    25861 cgtcaagaac tacggccttc acgtgacgcc gtccttgtcg ctcggtgagg atacgaccat
    25921 cgaccttacg tcgaagacgg ctttttatga catccgcggc ggagcctata tcgacgccgc
    25981 ccgtgaccac agcagcgacg aagatacggt cctcatggcc catctgggcc gcaagatcgg
    26041 cagtcatcac gaaatctacg gcgccgtcaa gttcatgccg tcttcgctgg actggaattt
    26101 cattcccggc tacttttacc gctttgccgg cagaacacag gcgggatacc tgttcgagag
    26161 cctcgacgac agccagcacc tgtggnnnnn nnnnncgggc ggtggacctt gcgctacgac
    26221 cgggacctga cgcatggcga ttccgaagtc ggcctgcact atcgcgtcga tgattatgtc
    26281 ggtttggaat atatcgtgtc cgaccacgat gcttggctcc ggattatcgg ttatttgtaa
    26341 tacaaaagga gaagttttat tatgatgtct gaaataggta agaaacgcaa tatcaagatt
    26401 ttttcggctg ttatcgcagc tatcttcgtc ttcagtgtcg ccggcctggc tatcatgcag
    26461 acgggcaacc ctgtcaatgc ggctccgtcg agcaacatcg gcgtcgtcga tacgagcaag
    26521 atcatcactc ctgataatca ggacgccgtt gcggcacaga aacagctcca gcaggctggc
    26581 gaagacatgc agaaacagtt tgaacagcag tcggccggca tggacgacca gcagaaacag
    26641 cagctcttcc agaagatgca gggcgaactg gctacgaaac gccaggaaat cttcaaaggc
    26701 atcaaagata aagtcgacac ggctgtcagc gatgttgcca agaccaaagg cctcagcctc
    26761 gtcgtcgaca agagcgtcgt cctctatggc ggtacggata ttaccgacca ggtcgctaaa
    26821 gaactgaaca agaactctgg ttccagcagc tccgacagca gcgccaacag ctccagcagt
    26881 tcggacagct ccagcagcag taaccagtaa ggagtcttta tgaaacggat atatgcggca
    26941 gccggtgccg cggtcctcat ggtctgtctc ctcgccggct ggtggttctt cagccaccgc
    27001 agccagccgg ccccgtcgac agctgcgacg ccggtctatg cctatgcaga cctggaacac
    27061 gtcgtcatga gccacccgcg ttatagcgag tatcaccatt tagaactgga atacaacgct
    27121 atggttgccc agtatcagtt tgaacagtgg aactattccc agaaagctgc tgccgaaggg
    27181 acgtcggtcc agaattttgc cgctgccgac gccgcaggta cggcagccct cgaccaggaa
    27241 ctgcaggcca aagtcgccct caaggagaac gagctcaaca accggctgaa acagcggtat
    27301 gattcgctcg tccaggaaaa gaagaagacg acgcctgtca tttcccaggc cgacacgttg
    27361 aaaatcgtca atctccagtt gaagctgaag accctgtccc tgtctaaaga agaacgggca
    27421 gcgacagagg aacagctcca aaccttgctc aaaggcagcc aggctgacgt ccaggtgacc
    27481 aaccggacag ccgatgaaat cgcggccctc atggcaccgg ataaggccca ggccaagaaa
    27541 gagctcgatg actacgccca gcaggtcaag aaagacctgg aaggacgcaa ggcagacaac
    27601 cagcaggtct tccagcagca gctgggcgtc ttgcaggacc ggccggaacc ggctgtgtgg
    27661 aacaaggaat ggaaagataa actcgatgca aaagaaaaag aaatgaaaga tgtgaaagac
    27721 gccattatgg cagatatccg cgataaagcc gccgatgtcg cccaggaaca gggctttgag
    27781 atgatcttcg ccaactacga aggcatcggc acggctacgg atgttaccga tgatattatt
    27841 gccaagttgg cataacagga ggcttttagc aatgaaaaaa gtttggaagc gcttaggaat
    27901 cgtcgccgca atgctggccc tgacgggtat gctcgccggc tgtggcggaa aagataatgt
    27961 aggcgtcgtc gacatgtcgc gcgtacagaa agaagcgccc ctggtccagc agtataaaca
    28021 gaagaccgaa gataaacaga aatccatcga aaaggaattg caggacgcac agcagtccat
    28081 gtcggctgaa gatttccaga agaaacagca gcaggcccag caggaactca atattttcgg
    28141 cgccagcatg cagcgtcagt tcatgtcgga catccagagc aagttaggcg acatcgccaa
    28201 ggataaaaat gtcggcatca tcgtcgtcaa agaagccgtt ccttccggcg gcatcgacgt
    28261 aaccgacgat ttaattgcca aattgcagta aaaaaagggg ttgtatatcg tgaagaaaac
    28321 agtacaggaa ttagcagtat tgctgggcgg cacggtcatc ggcaagggtg acgccgtcat
    28381 tgaagacgtg aaaggcctgg ccgaagccgg tctgcaggac attacctttg ccgtcgatcc
    28441 gtatacggaa tatttgccgc aggtccatgc cggtgccgtc attgtcgaaa aagaagttcc
    28501 tgccggagat aatacgctcg tcgtcgtaga aaatccccgc ctggcttttt cccagctcct
    28561 cgtgctgttt catccccgcc agtcggtcac gccgggcatc catccgacag ccatcatcga
    28621 cggcagcgcc tctatcggcg aagacgtagc catcatgcct tatgccgtca tcggcaagaa
    28681 cgtcgaaatc ggcaagggct cgacgattta tccctacgtc tttttaggcg ataatgtcaa
    28741 gatcggcgaa ggttcggtcc tctatcccgg tgccgtcatc cacgaaaact gcgtcctcgg
    28801 aaagaacgcc gtcatccggg ctcacgccgt catcggcggc gaaggcttcg gctttgctac
    28861 ggaaaagggc aagcatacgc ggatcccgca gatcggcaac gtcaccctcg gcgacgatgt
    28921 cgaaatcggc gcctgcacgt gtctcgataa tgcaaccctg ggttcgacga aagtcggcag
    28981 ggggacgaaa atcgacaacc tcgtccatct cggccacaac gtcgaaatcg gtgaagactg
    29041 tttcctcatc gcccagacgg gcatcgctgg cagcaccaaa gccggcaatc acgtcatctt
    29101 tgccggtcag accggctgca ctggccacat caccattggc gacaatgccg tctttgccgg
    29161 caaatcgggc attacgggca atgtcaaggg caaccaggtc tatgccgggt tccccatccg
    29221 gcctcacatg gaatggagcc gtacccaggt ctacctgaaa cacctgccgg aaatggccaa
    29281 gaccgtcaaa gccttgcaga aaaaaatcgc tgaactggaa aagaaattag aacaacagta
    29341 agaggatttg cctatgtaca cctttatgaa atgtctgagc tggatcatct gccacattcc
    29401 agaaggctgt cgccgggccc taggcacctt tttaggtgcc tttttctgga ctttcgttcc
    29461 caagaagcgc aaggtcctgg cccagcagca gattttggac tgcggtctga ccgatgatcc
    29521 ggaaaaggcc atggccatcg ccaaggccag tacggtccgg ttcgggccca tgatcatcga
    29581 agtcctgtcg tatccgcgct atacgaagga gatgctcgac gaaaagatta cctggcacgg
    29641 caaggaatac ctggacgaac tcaaagccag cggcgaaggg gccgtcttca tggcatccca
    29701 tgccggcaac tgggaactcc tgggagctgt cctggccatg aatggctatc ccctcatctc
    29761 cgttgctcag gaacagaaca gcaagagcgc cgataccttc atcaacgagt accgggccat
    29821 gatgaagcag cacgtcactt acaagacggg catccgcgac atggtccgct tcctccgcga
    29881 cggccactac atcggcctgc tcatggacca ggacccgggc tataccggca tcatggtcaa
    29941 actctttggc atggacaccc tgacggctga cggaccggcc aagatggccg gcatcgccaa
    30001 ttaccccatc gtgtcgacct tcatccacga agaccggccg taccaccacg tcgtcgaagt
    30061 cctgccgccc atcaagccgt acacagcgga ccacaagctc tcgaaagagg aaaagaaccg
    30121 catcatgtac gaaacgacac aggaactcaa cgaccgcctg gaagcccata tccgcaagta
    30181 tccagaagac tggttctggc tgcacaatcg ctggaaatgg actaaacggt ataaaataaa
    30241 acaagctgaa aagggggctg gcgtatgaag gcttatgcca tcatgcgtag tcactattac
    30301 ttcgtagttt gtagttcgga gtttgcagtt tgtagaaaat ggctctaaat ttaaaaccat
    30361 tcactaaaaa ctacaaactg caaacttaat actcaaaaag aggctgtctt cagggacagc
    30421 ctcttttttg ccttacaatc cacgacaaat ggggcgaaaa atgctatgat taaggaaaga
    30481 cattcttcac ttaggaggga cgatcgtgaa tattttctta tatctcatcg ctatcatctg
    30541 ttttgccgtt cccagttata actggttcgc tacgggtcat ctgtccggga cgttcagtct
    30601 cgtcttcggc gtcttcatcc tctactggat tcaccgccgc aataagaaga agttcaagcc
    30661 ggaactggaa ttttccagcg gcaacgaaga agtggacaag ctgaacgacg agctcttcag
    30721 caaagccgtc gatgatttca acgccattga aaaagaaatg aagcacgtcg atgacgacga
    30781 actgcggaaa cagctgcgca agatgcaggg cattgccaag aatttcctga cctacttgca
    30841 gcagcatccg gaacggatga gcctggcccg gcgcttcgtc gattattacc aggaccgggc
    30901 ccagctcctg gtccgcaagt accaggagct ggaaaagacg ggccttgaag cgcaggaagt
    30961 gcagcagtcg aaatgggaga tcaagcagct cctggataag ttcgacgaag cctatgaaga
    31021 ccagttctcc aaggtcctca atgcccagct catggacctc gacgccgaaa tgaaggtcat
    31081 gaagcagaac atggccgctg acggcatcca gacggaaccg cccaaggtgg aaccgcaggc
    31141 caagcgcagt gatgacctgg tcgggacgat catcgattta gccgaccagt tcttgaatac
    31201 aaaaagaaag taggtagata ctatggccga tatcaatttg aatgaccttt tagagaaaaa
    31261 gcaggccgac agccagaaag ccctgccagc cgtcgatgaa aagcacgaag tagctcattt
    31321 caatgcccag gtcgaaagcc tgacgccgga agaacggcag caggtcgaca ccatcaagga
    31381 tgccatcgac ctggtcaatt ccaacgccat cgtccagtac ggcagcgccg cccagaaaaa
    31441 tatcgccacc ttttcggaca gcgtcttgtc gacggtcaag agtacggacc cggccgcagc
    31501 cggtgacctg ctcaacgacc tggtcaagcg ggtcaagtcc tttgaagacg aaaacgacga
    31561 ggaccagggc ttcctggcca gcctgcccgt cgtcggctcg ctgttcaaga aaaaagaagc
    31621 cctgacccag tcctatacga cgatggccgc ccagatcgac a
//
LOCUS       DAWABZ010000004        31539 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_413_length_31539_cov_3.125778, whole genome shotgun sequence.
ACCESSION   DAWABZ010000004 DAWABZ010000000
VERSION     DAWABZ010000004.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 31539)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 31539)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..31539
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_413_length_31539_cov_3.125778"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    11889..11898
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    30522..30545
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6112 a         8385 c         9670 g         7338 t
ORIGIN      
        1 cttttcggtg tcgcggccta attccgtgcg gagagcgtta atcttggtcc agcgttcgac
       61 ttcgagagca ttgctgtcgc catcagcatg gagctgttcg gcagcgatga ctttcgagag
      121 gttttccgga atgatgcggt actggagttc cagtgcccag cggctgagag cgccgagttt
      181 gaaggattcc atgatgcgtt tctggaaagc gtcgcaagcg aggtcgtcca gtttttccgg
      241 atacgtcgta agggctttga agttttccca taccgtacgc ggagctttgc cgaacatggc
      301 attgcgttct tcgtcgctga agttttcgaa gacgtcgact tcggagcggt atgcccggtc
      361 tttttccagg taatctgcgt cttcgcccgg tttcttggag agttcagcca ggagttcagc
      421 cgtcgatttg ccgctggaga gagcgtattt gatgccgtcc agagcgccga ggtagcacat
      481 agcgatggcg atgtacgtat tggtgaacgg gttgggagcg cggagttcga aacgcgtagc
      541 tgccgggctg gtgatgtcac ggatgaggcc ggccaggacg gaacggttac gagtcggaac
      601 gtccggtgtt tcgccgccga gagccgtaac gatgcagaca ggagcttcga agcccggttt
      661 caagcggttg agggagtccg tcgtcgacga tacgaagggg tttatgactt cgtagtgttt
      721 gaggataccc atgatagcgc catagccgac ggccgagagg aattcgctct tcatgtcatc
      781 cgggttgaag aggttgacga atttgccatt cttcatgata gcgccgatgc cgagatgcgt
      841 atgttcaccg gagccggcaa caccgtggat cggttttgcc tggaaggtga cttcgaggcc
      901 gttttcgcgg aagacttcgc ggacgatgat acgggcagcg atttcgttat cggctgtctg
      961 tacagggtcg ctggcgtatt tccagtcgac ttcgagctgt tcgaggacga aggtcaattt
     1021 accggaatcg tcgatctggg ctttgacgcc gccgacttct ttatggccca tttccggttc
     1081 gagaccgtat ttttcaaggc gttcgacggt ctgttcaagc gccgtgcgga cgacgccgtg
     1141 tgtacgctgc cagtactgtt cctgcatctt ctgggatgcc gacaagaggc gggtagctac
     1201 gtcctggctc ggggtcttga cccagaattc caattctgta gccgtcgtga agacgatctt
     1261 ttcgatgtca tcaggattga tggacgtgcc tttgatgccg tatttcttga caagagccaa
     1321 aatctggtcg cctacgtagt cgcaggtgtt gcgaaggatg gaacgggagt cgataaagcc
     1381 cgtagcatgg cggaggaagc tcgggatacg gagcgtaccg acgggtttgc ccgtttccga
     1441 atcgatattg ccgtcattgt aatcgacgaa ccagtcgacg ccggggtcgg ctttgatgtc
     1501 gacacgagca ttggacaagg tagcgatgcc cggcaggaca acggaagaac cgtctgtctg
     1561 gaaagccgta gcattgaaga atttatcgta ttcatcgaag aatacactga taggaatctt
     1621 ttcgtccgta tcgttaccgg cgaggtcgat gcctacgaga gatacaaatt taatttccgg
     1681 gtgctgttcg agtaaagcca gcacaccttc tttaccatat tgacctgcag ggattacata
     1741 tactaaatca tctttagcca tcatgactcc tcctttgggt gaaaaaaaag actccgcccc
     1801 tgtggttaag gttcggagtc ttctttgact cttgtttcat gacctatttt attttcaaat
     1861 catgctaaaa taatacaatc aatcagtcta tttgtcaata gattttttag aaaaatctaa
     1921 caaaactaat tattttgtaa aatttctcga acaatctgtg aaacaagctt gccgtcggcc
     1981 ttacctttga ccttgggcat gacgagcttc atgacgtcgc ccatcttgag accgctctga
     2041 ccggcaacgg catcgttgac gatctggtgg atttcatcgg ctgtgagctg tttgggcatg
     2101 tacttttcca gtacggccat ttcggccttc gtttcttcga cgaggtcgct gcggcctgcc
     2161 ttttcaaact ctgccaacga ttccttgcgc tgtttcattt ctttggcaat gacggcaccg
     2221 acgccagcgt catcgagttc gcatttgccg tcgatttccg tattgcggat ggacgaacgg
     2281 accatgcgga tgacggataa agccgtttta ccggattctt tggctttcat ggccgctttc
     2341 atgtcctgaa gcagttcttc tttaagagac atgctatcac ccttcttaca tttgacgatt
     2401 atgctctgta cttccgtttc cgtgctgctt cggatttttt ctttctccgt acactcggtt
     2461 tttcgtagtg ttcacgtttc cgtacttcgg aaagaacacc tgccttttgg caagaacgtt
     2521 tgaagcggcg aagtgcgctg tctaacgttt cgtttttacc aactctgatt tctgccattc
     2581 tattatccct ccctccaagg tatgataggg agtgctttta ctgcacacaa tgaaattata
     2641 ctcagattag tgtcatttgt caacaatttc tgaggttcag cgtataaatt tactgaatac
     2701 ataattgcca tatcctaaac cgtcttcact gaggcagcag ccccgctcgg tgcgttccag
     2761 gaggccctgg cggatgaggc cgtcgatgac aggaccgaag tcggcttcga tggatttccc
     2821 gaagcgtctg gcataatcgc cgtaatcgat gccgacccgg cggcgcaggg ccaggaagca
     2881 gtaatcttcg acggcccgtt tggcgtcgat ggcgacggat tcggcgacga cactctccct
     2941 gccggcctta tggatatacg ccgggatgtt ggctatattg gcccagcgcg tcgtcccatc
     3001 gaaggaatgg gccgatacac cgaagccgat ataaggctca taagtccagt atttgctgtt
     3061 atggcgcgac tcgaaaggcc ctttggcata gctggaaatc tcgtagtgat ggaagccata
     3121 accgtgcatg gcgtcgtgga cggccttccc catggcttcg accgtcccgt caggcggcag
     3181 gtgccatgtc cccttttcca ggcccgcctt gagctgcgtc ccgtcttcga cgatgaggct
     3241 gtaaatggac gcgtggacca cgggcagctg ggaaaggcgc tctacgtcgc ggcggacgtc
     3301 ggccaggtgc tggccgggca ggccgtacat caggtccagg gacaaattat ggatgccagc
     3361 cgcgtaggcc gcctggatgg ccgcttcgcc ctgggctgcc gtatggaccc ggcccagaaa
     3421 ggccagcatg gcgtcggaaa aggactggac gcccaggctc aggcggttga cgccgcaatc
     3481 ggccagagcc gcggccttat cataatcgag actgtccgga ttggcttcta tcgtgatttc
     3541 cgcatcgctg gcaatgcaga aggtctgccg cagggtcttc aggatgcagc tgacgtcgcc
     3601 ggcttccagc attgacggcg taccgccgcc gacgtagacc gtatcagccc cttccccggt
     3661 atagggcgag gccgcgatgt cccggcacag ggcttcgaca tagccctggc agtatcgctc
     3721 caggccggca taggacggaa agtcgcagta caggcattta tggcggcaga acgggatgtg
     3781 gacgtacagg cccagcgtca ttcttcgtcg actttgagga cggccatgaa ggcttcctgc
     3841 gggatttcga cggtaccgaa ctgtttcatg cgcttcttgc cttccttctg tttttccagg
     3901 agcttgcgct tacgggaaat atcgccgcca tagcacttgg ccaggacgtc cttgcgcagg
     3961 gctgccacgt tttcgcgggc aatgatctta ttgcccacag cggcctggat ggggatctgg
     4021 aagagctggc gcggaatgag gctgcgcagt ttttcgacca gggcgcggcc gcggcgggcg
     4081 gcgaaatcct tgtggacgat gacgctcagg gcgtcgacga cgtcgccgtt gatgaggatg
     4141 tccattttga ccataggcga ggcctggtag ccgttgagct cataatcgag ggacgcatag
     4201 ccgcgggtcg ccgacttgag gacgtcgaag tagtcgtaga ggatttcaca caggggcagg
     4261 ttgtagatca aagaaacgcg cgtgtcgtcg aggtaatcca tggtgacgta ctgaccgcgg
     4321 cggttctggg agatttccat gaccgtgccg accatgtcct tggggatgat gatcgtcgtc
     4381 ttgaccagcg gttcttcgat gtggtcgatt ttcgtcatgt ccggcatctg cgacgggttg
     4441 tccacttcga ccatggtgcc gtccgtgcag tagacgtggt aaatgaccga cggcgccgtc
     4501 gtgatcaggg acaggttgta ttcccgttcc agccgttcgc ggacgacgtc catgtgcagg
     4561 agtcccagga agccgcagcg gaagccgaag cccagggccg acgacgtttc cggttcaaag
     4621 agcagcgccg catcgttgag gtggagcttc tccagggcat cgcggaggtt gtcgtagtcg
     4681 ttcgtttcga ccgggtagag gccgcagtag accatgggca cagccttgcg gtagcccggc
     4741 agggcttctt tggccggatt gtcggccagg gtaaccgtat cgccgacgcg gcagtcgcgg
     4801 acgttcttca tgctggctgc cagatagccg acgctgccac attccaggga cgggacggga
     4861 tgcatctggg gcaggaagac gcccgtttcg atgacttcaa attctttgcc cgacgccatc
     4921 atgcggatgc gctggcccgg cttgacgctg ccttccatga tgcggatatt ggcgatggcc
     4981 cctttatagg catcgaaatg ggaatcgaag atgagggccc gcaggggctt gtccccttcg
     5041 tcagccggtg ccgggatgcg ctggacaatg gcttccagga ccttgtcgac gccgtagccc
     5101 gtcttggcgc taatcatgat ggcgtccgat gcgtccaggc cgatgacgtc ttcgatttcc
     5161 ttgcagaccc ggtccgggtc ggcgctgggc aggtcgactt tattgatgat gggaataatt
     5221 tccagatcgt gttccagggc caggtagacg ttggccaggg tctgggcttc gacgccctgc
     5281 gtggcatcga cgatgagcag ggccccttcg caggccgcca ggctgcgcga tacttcgtag
     5341 ttgaagtcga cgtggcccgg cgtgtcgatg agattgagcg tatactgttt gccatctttg
     5401 gcatcgtaaa tgagacgggc cgactgggct ttgatggtaa tgccccgttc ccgttcgaga
     5461 tccatcgtat cgaggagctg gtcttccatt tcccgtttgg gcacggtgcc ggtcatttcc
     5521 agcatccggt cggccagcgt cgatttgcca tggtcgatat gtgcgatgat tgaaaagttt
     5581 ctgatatgat ctgtagacac aggaatcctc ctcttttttc ctattggatg acgccgccgc
     5641 ccaggacgcg ttcgcctgtt tcggcgtcgt aaagtaccag ggcctggccg gccgtgacgg
     5701 cgcgctgggc atcgtcgaat tcgacccgca ggctgtcgcc gtcgggatag accgtgcagg
     5761 ccgcttcacg ggcagcatag cgtatcttgc ccagggcccg gaacggtgcg gacggtgcct
     5821 gttcgaccca cgtcgcttca ccggcatgga cagtcttcga gaacaaatcc tgttccgagc
     5881 cgacgacgac ccggttatgg gccacgtcga gggccgtcac ataatacgga ccgccgggac
     5941 cgccgaggtt caacccccgc cgctgaccga tggtataggc ggcaatgccc ttgtggcggc
     6001 cgatgacgcg gccgctctgg tcgacgaagt cgccgggctt gaagatgccc ttgcgttcgt
     6061 gctgcaagaa gcccttatag tcgttgtccg ggatgaagca gatatcctgg ctttcaggct
     6121 tgttgaagac cggcaggccc cattctttag ccagttcccg cgtatggacc ttttccaggc
     6181 cggccatggg gaataagaga tggggcagca ggtcctgtgt cagctgatag aggacatagg
     6241 actggtcctt gcggcggtct gtgccgcgga gcagggaata gattcccgtc ccttcgtcct
     6301 tgacgatgcg ggcgtaatgg cctgtcgcca ggaagtcggc accgaactgc ttggcctttt
     6361 cccagagcag accgaattta atgaatttat tgcaggcaat gcagggattg ggcgtcctgc
     6421 cgtggacgta ggcatcgacg aagtagtcga tgacgtcgcg ccggaagata tcgcggaaat
     6481 ccagggtata atggggaatc cccaggaccg aggcgacggc tttggcatcg tcgacggaac
     6541 tgagggagca gcaggcgtgg acgttgtcga tggtgcaggc cgggtcttct tcccacaggc
     6601 gcagggtaat gccgatgacg tcatacccct gctgcttcaa aaggccggcc gtgaccgaac
     6661 tgtcgacgcc gccgctcata gctacggcaa ctcgtttcat agtgactctc ctctgctttt
     6721 acggacagcg tcgccgatga tggcgatggc tgcttcgatt tcatctaagg tattttcttt
     6781 gcctacggac aggcggatgc tgctgcgcag ccatttgtcc tcaacgccca tggcctggag
     6841 gacgtgggac gcttcgacgg cgccggcttc gcaggccgaa ccagccgata cggccagacc
     6901 ctgcaggtcg agggcgataa gcaggatggc gctgtccacg ccgtcgacgc tgaagtcgat
     6961 gatgttgggc aggacgtggt tgcgccggcc gttgaggtgg aagcgggcgt cgcccttgat
     7021 gagccggtca tggacggcat cggtcaggcg ccgcgccgcc cggacgcggt cgtcccgttc
     7081 ggcctccagg agttcggcag ccttgccgaa tccggcaatg gcggcgacat tctccgtgcc
     7141 cgcccgcagg cggtgttcct ggggtccgcc ataggcttcg gcagcgacct gcagtccgtg
     7201 gcggatgtac aaggcgccga cacctttggg gccgtaaatc ttatggctgc agaccgtgag
     7261 catgtcgatg ccctgttcca gcggccggat aggcacgtag ccaaaggcct ggacggcatc
     7321 gacgtggaag gcgatgccct tctgccgggc cagggcgccg atggcgtcga tgggctggac
     7381 catgcccgtt tcattattgg catacatgac ggaaataagg accgtatcat cgcgcagggc
     7441 ctgggccaca gcgtcggctg tgacccggcc gtcggcttcg acagggacat aagtcacgtc
     7501 atagcccttc ccttccaggg cctggaaagt ccgcaggacg gcgtggtgtt ctacggccgt
     7561 cgtgaccagg tggccgccgt gggggtaatt ggcccgcagg aagccgagga tggccaggtt
     7621 gtccgcttcc gtgccgccgc tggtgaagac caggtcttcc cctttgacgc ccaggcagtg
     7681 ggcgacctgg ctgcgcgccg tctggacggc agaccgggcc tgctggccga aataatgaag
     7741 gctcgacgga ttgccgtaag ccgtcgtcaa atacggcatc atgacgtcca ggacgcgcgg
     7801 gtccatcggc gtcgctgccg cgttatctaa ataaattcgt ttcattgatg attttgactc
     7861 caaagttgac aaaatagcta taaaaaatag cctattattt ataataccat aggtagttgt
     7921 tggtggctag tggctagtag gggccgtccc aaagggcggc ccgcttgtgg gaatcccgtt
     7981 tgctggcggc ccgttgcggg aatcccgcgg gacgcaaatt caacctctca gtcagctgac
     8041 gctgacagct cccctataaa gggagccttg ggcgggacgc ctacgattgc ggtccgtggg
     8101 accgcggcgg actgcggact gcgaccggcg acctgcgtta tccacaggat tcgcggcggg
     8161 ctccccacgt gggagcccct acagttacta tgccagacta gtgtctggca tagtaacttg
     8221 gtacgccgtc gacgtcggcg catttggtat tacagccttc ctggctgcag acccagaagg
     8281 ggccgtaggg gccgctgcgt ttctggaagg tccctttgtg gcagcgcggg cacgggtacg
     8341 gcgacggttt ggcgtcgggc cgtttctttt ctccgtcgac gggttcggtg tggcggcacg
     8401 acgggtagtt gctgcagccg tagaagtcgc cgtatttgcc gtggcgcttg accatggcac
     8461 tgccgcagac ggggcaggtc ttgatgccgg ccatgtgcat gacgactttc cgggccgccg
     8521 tgaccagggt atccaggaag gacagctggt cgtggaggaa ggtgtccagt ttatccttgc
     8581 ccaggctcat ctgatagagc cgttcttccc agacagccgt ttcgtcggga tagagcattt
     8641 catcgggcag ggaatcgacg aggaggtagg cgtcatctgt cggcttgagg tacttcttct
     8701 tgccttccgt cgtcaggaat ttgcgctgga tgagctcctg gataatcgtc gcccgcgtcg
     8761 cttctgtgcc gatgccctgg acttctttca atttcttctt caggctctcg tctttgacgt
     8821 acttataaat ttccttcatg gctgccaaga gggtcgacgg cgtgaagcgc ggcggcggct
     8881 tagtcgcctt tttgtcgacc tggccgttct gataatggac gccatcgccc ttgcggacag
     8941 ccggcagggt ccccttctct tcttcgtcgt catcgtgctt gtcgctctga tagaggacct
     9001 tccagcccgg cacggtgacc gtcttgccat gggcgacgaa gcgttcatca gccgcttcga
     9061 tgaccatgcg cgtctggtca tattcatgga tgggatagaa ttgggcaata taggcctggg
     9121 cgacgaggta ataaatatgc tgctgcatag acgtcaattt atcgaaggga caggctacgg
     9181 tcgtcggaat gatggcgtga tgggccgtga tcttgctgtc gttccaggcg cggctgcgta
     9241 tcttgccgtc ggcaccggcg acccattcag ccagcttccc tgccggcagt ttcccgagat
     9301 tggccaaaat atggggtaca tccttgcgct ggttcaccgg caggtattcg cagtccgacc
     9361 ggggataggt cgtcagtttc cgttcatata attcctgagc cgtatcgaga acctgctgcg
     9421 gtgtatagcc ataacgcctg cctgccagga cctgcaggga cgacagggac aggggcaggc
     9481 gctgggcttc ttttttcttg gtctttttat gctcgacgat ggttccgtca ggccgttcct
     9541 gcaggcgttc gaggatggac tgggccgtct tttcatcgac acagcgccct tccgggtcga
     9601 ggccgctctg ggtatccctg ggctgccact gggcgcggat gaggccgttc ttatgaagga
     9661 aatcggcttt gacgacgtaa tacgtgacgg ggacgaagtt gtccaattcc cgctgacggc
     9721 ggacgacgag ggccagggtc ggcgtcttga cgcggccgat gggaaagacg acgcggtgtc
     9781 cctgacggcg ggcggccagg gtataggccc gggacagatt catgccgatg agccagtcgg
     9841 cgcggctgcg ggccagggcc gattccttga gatggacgaa atcgccgttg ctgcgcaaat
     9901 cgtcgaggga ttcgtggatg ctcttctcgt cgagggcatt caagaggatc cgttcgacag
     9961 gcttcttatt gccgacgtaa tcgaggatct catcgatgag cagctgccct tcccggtccg
    10021 ggtcaccggc gttgacgata gtatcgacgt cggggttggc gatgaggtcc gtaatgacct
    10081 tgaactggtc cttactggcc aggctgacca ggagcttcca ctgcttgggg ataatcggca
    10141 agtcgtcggc gttccagcgc ttgtacttgg gatcataatc ctgaggctcg gcctgctgca
    10201 ggacgtggcc ataagcccag gtcacgatgc cgccgcccgt gcggatacag ccgttccccc
    10261 gctggtgcgg atcaggcagg cacttcgcca attcccgggc catgctcggc ttttcggcaa
    10321 taaaaagttt cataaggcat ctctctttct gaaatagact gtaccttata tcataccatg
    10381 gttggggatt agtggctagt aggggccgtc ctgtaaaggc ggcccgcttg tgggaatccc
    10441 gtcgaatcgt ggctcgtggc taggggctcg tggttcgtgg tccttctctt tatagtttgt
    10501 agtacggagt ttgtagtttg caggagatgg ttttaaattt ttaaccatgg tctacaaact
    10561 aaaaactaca aacttgaacc ttacacgtga atgcaaattc aacctctcag tcagctgacg
    10621 ctgacagctc ccctataaag ggagccttgg gcgggacgcc tacgattgcg gtccatggga
    10681 ccgcggcggc ctgcggtccg cgtttattcc cattattcac agcggtctcc ccacggggag
    10741 cccctacgaa agtcaccaaa gccgttacac ggctttggtg actttggcca gctggccggc
    10801 ggcttcaatg gcttggacga aggccggcgg gatgtcttct tttttcaggg actggccgat
    10861 gaggcggccg aggatgacgc ctttgttgct gtagagccag gcgaagtaat agatgtacag
    10921 ggccttttta ttacgcgggt tgacgtgcca caagaggcga gccgggtaat cgtggaagtt
    10981 gacgtccagt ttgcggcagg cctttttgat catgtccgtc gtggcccggg cgtgttcgcc
    11041 gcaggccgtg ttgatgatgc ggtccaggtc gcggcgctgg ccgtggtcga tcatggtctt
    11101 ggccaggtcc atttcaaagc agatttcgtt ggtgaagtag acgccccgtt tgcctttgtg
    11161 gctgcccttg acgtcggcat cgtagagggc gacgacggga atcttgaagt attccaggag
    11221 tttcttgatc ttggagatgg aactttcgcc gcgggcattg atgaggcaga tgccgtagta
    11281 gtcgaagggc aggtccaggg tcttggcgaa gtgctggaaa cagccgtatt ccgtttcccc
    11341 ttcgacgatg aggacgctcc gcgaatagag ggcttccttg acttccggga agtgcatgat
    11401 caggtgtttt tcgatttctt cgctgaagtg gaagccagcc ccgcaggccg ctttgaccag
    11461 gccctgcttg ttgcggtaca ggcggatgat gtggcggtag tcgtcgatga gggaatccgt
    11521 cgagtgggtg acgacgaata actgcccccg caggccgtcg aggttgaaaa ggtccttgag
    11581 gagcgcacag aactgcgggt cttcattgtt caggagctgc tggtaataat gcaggaccga
    11641 gcgctgcatg tagggatgga ggtgcatttc cggttcatcg atggagatga tgaggggcag
    11701 gtagcgcttc ccttcttcgt cgacgatgac gttgtgttcc aacggtacag cgcggctgat
    11761 ggacatcatg tagacctggg tgatgacgca cagggcggcc agggaaatga atttcatgtt
    11821 gtccgccctg ggccggtcca tgcggcagag catgcgggac aagaggaaga tgttgacgaa
    11881 atacgatgnn nnnnnnnnac gatgaatcga ggctgccgac ggcgacttcg tgatggatga
    11941 aggccttggc ttcttccgac aggccgtccc agtgagcttc gctccattgg gagagcttct
    12001 gttccagggc gcggtaaata cgcggcggca cgcgctgttc ttccggcatg gccgccgaat
    12061 aggacacgta gcgcaggcgg cggatgagtt ctaacggcag ttcttcgccg ctgttgtcgt
    12121 cgtagagatg gggatacatg tcgctgacat gcatttccat gcggacgcgc aggctctgac
    12181 ggtgctcgcc cggcgtatcg gcgaaatact ccttctcgct ctggagcagg tgcaggtcta
    12241 aggtgatgac gatgggccgg gtcacgtcgg cgtagtcgtc ttcttcgaca ttgcggccgg
    12301 cactcatgta ggaaatgagg cgcagcaggc cgctcttgcc aatggcgttt tcaccgacga
    12361 tgtagttggc cctttcgtgg aaagtcattt cgacgtcttg tagattccgg taattttgaa
    12421 tacgcatgta agtaatatac acaaaatcac atccttttca acattacttt tactatacgt
    12481 attcgacaaa aaacgcgcta ctcccttctt ttagagagca gcgcgttatt ccttttacgt
    12541 atcgttcatg gtttgatgaa cggcctatgg gttcagcaaa catcgtgaaa aacgatgtga
    12601 ccaatctctg gcgggcgccc cacgtggtcg cccctacgat gaatggccta cgacgaacga
    12661 cctacgatca acgtccgctt tcgcggcaca ctttggccag ccagtgggcg atgtcgaagg
    12721 tcgtctggcc ttctttgagc tgccagtgcc agttgccttc ggcgacaccg ggcgtgttca
    12781 tgcgtcccga cgacggcagg gccaggaggt cctgcatggg gattatgacc gtttcggccc
    12841 ggcggcggta ggtataggcg atgagggcgc cgacgatttc ctgcggtgcc gcttcatcgg
    12901 acaatcctag catctggcgg acgtggagct gctggctcgg cgtcaggtcg tcttcatacc
    12961 agccgcggat agtgttgttg tcatgggtcc cggtataggc gatgcagtcc ggctcggtat
    13021 cgaaggccag gcggccgtcg gcccggtcct tgaggtggaa ctggatgact ttcatgcccg
    13081 gcaggtgcag ggcatctttc aaggtgcaca catcgtcggt gatgacgccc aggtcttcgg
    13141 cgaccaggcg gatcctgccg attttctgat agaccgcccg gaagaggtcc aggccggggc
    13201 catctttcca agcgccgctc ttggccgttt ccgaaccggc cggtacggcc cagtaagcgg
    13261 caaagccgcg gaaatggtcg acccggactt cgtcgacgag gtccatcatg cggcggaagc
    13321 ggtctgtcca ccactggtag ttatccgcag ccatggcctg gtaatcatag aggggattgc
    13381 cccagagctg gccgtcggca ctgaagtagt caggcgggac accggcgacg gacgacggat
    13441 tccctgcttc atcgaggtcg aactggtcct gatgggccca gcagtcggca ctgttatggg
    13501 cgacgaacat gggcacgtcg ccgaggatgg acacgccgtt ttcagcggca taggcccgga
    13561 catcgtgcca ctggcggctg aagatgtact gcatgaattt gacatgcgat acggcgccgg
    13621 ccagctcctt gccatactgg gccagggctt cccggctgtg atggcggatg tcttcgggcc
    13681 attccgtcca gcaacggccg ccgaagaagt cgcggacagc ccggaagagg gcgtagtcat
    13741 cgagccagta ggcattcttt tcacagaagg cccggaacgg ttcccaggga atggtcaggt
    13801 ccggatcatg tgacatatcc cagaggagtt tggccttata ggctgccagg ctgtgccagg
    13861 acgccgtctt tttcccctgt gccaggaaat cgtctaaggc ttcctgtttc agccagcccc
    13921 agtcgcgcag gacttccagg gaaatgaagg cttcgttgcc ggcaaaggct gattccgaca
    13981 gatacggcga atcgcccatg agaggcggcg tcaggggcag gatctgccag acccgctggc
    14041 cggcggcctt gaggaagtcg acgaagcggt acgctgccgg ccctaagacg ccgttgccgc
    14101 aggcagccgg cagggacgtc ggatgcagga ggacaccggc ccgcttggtc ccggcatctt
    14161 tcccctggag gatgaccgct ttcaacggcg gcaaagttag ggtgaagcgg ctgttcacgg
    14221 tcgtgaccgg cgtcatgcgc ggatggaggg cattggtcag tttgccgtag gccaagccgt
    14281 ccgtatagag gctgatgttc cgcagggccg acgggttgcg gttcatggcg atgatgaaga
    14341 aaccgtcttc agcgggcttg ccgaagacat cctttccccc ttcgatgacc cgggcaaagg
    14401 caaagacgtc gccatcggcg tagagcggga tgaaacggcc cgtctgtaaa gcgtcgtgtg
    14461 ctgtccgcag ccgcgtcagg gccttggtcc aggccaggag gtccaggtct tcatggcccc
    14521 agggataggt ccggcggttg tccgggtcct tgccgcccgt gacgcccact tcgtcgccgt
    14581 agtagatgca cggcgcaccg ggcatggtca tctgccagtc tgccagcagt ttcaggcgct
    14641 tccggcctgt ttcttcagct gtggccacgt cggtgccgcc ggcccgggtg aggaccgtca
    14701 ggatgcgttc gatatcgtgg ctgctgatga ggttgagcat ggcatagaaa ttttcaggcg
    14761 ggtaattttc gatgagccgc gtcagttcgg cattcatgcg ccgcgcatct ttggcgccgg
    14821 tcatgaagtc gatcatcagg gagcgctggg cgtagttcat ggcgctgtcg atatcgtagc
    14881 cgcagaggta ttcccgctgt tcgctgtagc tgaccttgtt cgacgcatct tcccagactt
    14941 cgccgatgag gacggcatcg ggcttttcct ctttcagggt atggtagaac tggcgcagga
    15001 agggtacggg cagctcgtcg atgacgtcca gtcgccagcc gctgatgcct tcgttcagcc
    15061 agtatttgag gacgctgtct tcgctgttga tgatgaaatc catgtacgac ggcgtcgttt
    15121 cttctacgtc cggcaggtcc gatacgcccc accacgattc atagtcgtcg ggataatgct
    15181 tgaagcggta ccattcatag tacggcgact ccttcgactg gtaggcgccg acgccgggat
    15241 agttgccgaa gcggttgaag tagatgctgt cggcaccggt atggctgaag acgccgtcga
    15301 ggatgatatg gatgccttct tccctggcag cctgacagaa ttcggcgaac tcttcattcg
    15361 tccccagcat ggggtcgacg cggaagtaat cggccgtgcc gtaacggtga ttgctggaac
    15421 tttcaaaaat cgggttgagg taaatggccg taatgcccag ttccttgaga tagggcaatt
    15481 tttcccggat gccggcgatg ttgccgccga aaaagtcgta atagacgatg tcgccctggc
    15541 cttcgcgctt gcagtaatag ggcacatcgt cccagtcgct gtggatgacg gcgcccttct
    15601 tgccgtagag gcggcccgac gtatgggtac cgtggcggaa gcggtccggg aagatctggt
    15661 agacgatggc gtgcttgaac cagtccggcg tatgggcgtc cttatcgtag accgtgacct
    15721 gataggccgg cggcacgtcg ggatatttgg ccccttcgcc gccgaggcgg tcgtagttgt
    15781 tgccatagta gaaggtccta tggccatcgt cgatgatgaa ataataccag agcagacagc
    15841 ccttgggcgg aatggtgacg atgcccgtga agatgccgtc ttcataactc atgggtacaa
    15901 aggattcgcc gacattgatc tgccacaggc ggatgcgggc ccggcagttc tgagcatcgc
    15961 tgccgctggc acggaggcgc aggatgagcc ggctcccgga aggaacggcc ccaaaagacg
    16021 agcggtaata gaattcgtga gagttgtggt acaatcgtaa gtccataagc ggtcctttcg
    16081 tggttgggga aagtaaaaga ggctgtccat gaagacagcc tctttttgag tattaagttt
    16141 gcagtgtgta gtgaatggtt ttaaatttaa agtcatctgc tacaaactaa aaactccgta
    16201 ctgcaaacta taagtcaaag ggactatgca taatggcaga agccttcatg cgacagtccc
    16261 ttttgagttt gcagtcttta gtttttagtg aatagctgga aatgcaaagc caatttctac
    16321 aaactacaaa ctctgaacta caaactgtct tttattgttg taatactttt tcgtacaggt
    16381 tctggtaggc ttcggccgag cggtcccagc tgtagtcgct ggtcatggcg ttgcggacga
    16441 ggatatccca cagggccgtg tcttcatagt aacccatggc ccgtttcgtc gtgaacagca
    16501 gttcgtgggc gttgaagttc gggaaggtca ggccattgcc ctgtttgctg tatttatcga
    16561 agggatggac cgtatccttg aggccgcccg tttcgcggac gatgggaatg gcgccgtagc
    16621 gcagggcgat gagctggctc aggccgcacg gttcgtaccg cgacggcatg aggaacatgt
    16681 cggcagcggc gtagaccttg tgggccaggg cttcgttgaa ggtgatgttc gccgatacct
    16741 tggtcggatg gcggcgggcc agttcgcgga aggcttcttc atagccggca tcgcccgtcc
    16801 cgaccaggac cagctggacg tcgtcgtcga cgagttcatc catgatgcgc gtgaccaggt
    16861 ccaggccctt gtcttcgacg aggcgcgtga ccatggcgat catggggacg ccccgcttga
    16921 tgcgcaggcc cagttcggcc cgcagggcat ctttattttc ccgcttgcca gcccgggcgt
    16981 tggcgacgga atactggcgc ttgatatacg ggtccgtttc cggattgtag acgtcgatgt
    17041 ccaggccgtt gatgatgccc gtcacctttt ccccattgtc gcggatatag ccatccaggc
    17101 cttcgccaaa gtacggatag gtgatttcct gggcatacga cggactgacc gtcgtgatgt
    17161 ggtcggcgta gaccatgccc gacttcatgt agttcacgca gccgtcacat tccaggctgc
    17221 cgttgtcgaa gagttcctgc ggcaggccaa tgatgtcgcg catgatcgac ggcggataga
    17281 tgccctggta tttcaggttg tgaatggtat agatgacttt gattcccttg tagaaatcat
    17341 cgtgccagaa atcttctttg aggaagacgc ccatgaggcc cgtatgccag tcgttgcagt
    17401 ggatgatgtc cggcttgaag ccgatcttgg gcagcatggt caggacggcg cggcagaaat
    17461 aggcgaagcg ttcgtcgtca tcgccgtagc cgtagaggcc gtcgcgcttg aaataccatt
    17521 cgttgtcgat gaaatagacg gtcacgccgt cctgcttgat ttcttcgaca ccgtagtaga
    17581 gctggcgcca gaccaggttc acgatgcctg tcgtcacttc tttcatctgc ttcttcgctt
    17641 cttcagcgat gcggctgtat ttcggaatga ccaggcggac gtccatgccc ttcttggcta
    17701 aagccttggg cagtgcgccc atgacatcgg ccagcccgcc tgttttgata aatggcactg
    17761 cttctgaggc cacaaataat acattcatcg tatgtccccc actttcccta agtaccgtat
    17821 caggcgtctg cttccttttt ggcaggacgc ccagttcttc tctttctcgt tgcagcagtc
    17881 ttcttttcag acgctgccgt cttggctttc ccttttccac gggtcgtcgc cttctttccc
    17941 ggcgccttgg ccgttttctt taccgtatcg tcggcagact tatcggcttt cactgtccgg
    18001 cggcgccgcg gtttggtcac tgcttttttt taacactccc tgtcagctgc aggtaaatcg
    18061 tcgccagcgg cggcaaggtc agttccaggg actggtccat gccgtgcatg ggcacatcct
    18121 gggcctcgaa ttcgccttca ttgaggacgt tggagccgcc gaaggccgtg gcgtcactgt
    18181 tgaagacttc gacgtacgtc cctttccggg ggacgccgaa gcggtacgaa tggcggacaa
    18241 ccggcgtgaa gttgcagacg acgacggtca tgtccgtttc gtccttaccc gtacggtaaa
    18301 aggcgatgac gctgttgtcg cagtcgtcgc agctgatcca cgagaagccc gaccagtcgc
    18361 agtcttcttc ccagaattcc ggatggtcca catagtacgt attcaggctg cgcacgtagt
    18421 cgtgcatggc cttgtgcatg ggatacttga ggaggtgcca gtccaggctg tcatcgtatt
    18481 tccattcgat gaactggccg aactcgctgc ccatgaacag aagcttcttg cccggatggg
    18541 acatccagta gccgtaaaag gcgcgcaggc cggcgaattt ctgccagtag tcgccaggca
    18601 tcttgtccag gagggaatgt ttgccgtgga cgacttcatc atgcgacaag ggcaggatga
    18661 agttttctga aaaagcatag cataacgaaa acgtaatcag gttgtgattg tccttgcgga
    18721 acagcgggtc cagcgacatg tagcggagca tatcgttcat ccatcccatg ttccatttgt
    18781 aattgaatcc cagtccgccg acgtcgacgg gcttggtcac gagaggccag gccgacgatt
    18841 cttcggcaat catcagggcc tgagggtatt ccttgaagac ggccgtattg agatggcgca
    18901 ggaagtcgat ggcttccagg ttttcccggc cgccgtactt attgggctgc cagtcgccgt
    18961 cggcatggcc gtaatcgaga tagagcatgt tggcgacggc gtcgatgcgc aggccgtcga
    19021 tgtggaactg gctgatccag aacagggcac tggaaatgag gaaggactgg acttccgtcc
    19081 ggccgtagtc gaaattcatc gtgtcccatt ccttgttttc tgccagcatg gggttgcccg
    19141 attcatagca cggttcgcca tcgaaatggc gcaggccgtg ggcgtcgcgg caaaaatgcc
    19201 ccggtaccca gtcgaggatg acgccgatgc cgttgcggtg gcagaggtcg acgaggtaca
    19261 tgaagtcttc cgggctgcca tagcggctgg tcgccgagaa ataccccgtc ccctggtagc
    19321 cccaggaacc atcgaaggga tattcgcaca gcggcatgag ctcgatgtgg gtataattca
    19381 tctgtttgac ataatcgacc agctggggcg cgatatcgcg gtacgacaac atttcgccgt
    19441 cttcgccgcg gcgccacgag ccgagatgga cttcgtaggt cagcatgggc ttacggtacg
    19501 aatcgtaagc actgcggctg tccatccatt tcttgtcgcg ccacgtgtag tggagcttgg
    19561 tcagtttcga tgccgtattg ggacgtactt ccgaccagaa ggcataggga tcgctcttta
    19621 agatgcgttc gcctgacgcc gtcgtgatgg cgtacttgta gatgtcccct tcctggaggc
    19681 cggcaatatg cataacccaa atgccgttgc cgcagctgct ccgctccatg ggattggcat
    19741 cggcgtccca gtgattgaaa tcaccgacga cgctgacctg ctgggcgtga ggcgcccaga
    19801 cggcaaagcg cacgcagggc ttgcggcgca ggaccgtgaa atgggccccg aacagacgat
    19861 aactggcgta gttcgtaccc tgatgataca agaaggtact gtactcgtcg attgcactga
    19921 gtttcatatc ttatcctcct gattcttagt tagtgtttga ccggcttgat gcgccaaatg
    19981 tcatcggcgt actgctgaat cgtccggtcg ctggagaagt agccggactg ggcgatgttg
    20041 acgacgctcg aatgacacca tttcgtatgg tcgccgtagc gtttggcgat ttcctgctgg
    20101 gcctggcagt agggctggaa gtccttgagg acgaagtatt cgtcgttccg gtcgaggaag
    20161 gccgagtaga gcataccgaa gtggtccccg ccgatagagc cgtcgataga gccgtcgacg
    20221 agggcatcca tgaggcggcg gatggcggcg gcgtcgctgt acatctgcca cgacgagtag
    20281 ccgccgtgga tgtagtagtt catgacttcg tcagcccgca gcccgaagat gacgcagttt
    20341 tcgtcgccga ccttgcggtg gatttcgacg ttggccccgt ccatggtccc cagggtgatg
    20401 gcaccgttca tcatgaactt catgttgccc gtgcccgacg cttccttgcc ggctgtcgaa
    20461 atctgttcgc tcacgtcagc agccgggaag aggagctggc ctaaggaaac attgtagttt
    20521 tccaggaaca ggaccttcat ctgcttgttg accttacggt cggaattgac catatgggcg
    20581 acgacgttga tgaggcggat cgtttccttg gcttcgccgt agccggcagc ggccttgccg
    20641 ccaaagagga aggtccgcgg cgtgatcttg agtttcgggt tgtccttcaa gaggtagtac
    20701 aagtgcagga tgtgcaggat gttcaaggtc tggcgcttgt acaggtggat gcgcttgatc
    20761 tggatgtcga agatcgagtc cgtccgcacg tccgtatcgt agcggtcgcg gatgtagcgg
    20821 gccaggattt ccttgcggtc ctttttgacc cgcgtcagct gttcctggaa ggccgggtcg
    20881 tcggcaaagg gcatgaggtc ctggagcttt gacggcgtcc ggcgccagct gtcaccgatg
    20941 gtatcgtcga tgagcgacgc cagctgcgga ttggcttcga tgagccagcg gcgatgggaa
    21001 atgccgttgg tcttgttcga gaagcggtgc gggaagcacg tgtagaactg gtgcagcgtc
    21061 gaatccttga ggatctgcga atggatttcg gcgacgccgt tgacgctgtg gctgccgagg
    21121 acggccagat gagccatgtg gacctggcca tcccagagga cggcgacgtc gcgggctctc
    21181 gattcatcgc ccggatagag gctgcggact tctttgagcc agcggttgtt gatttcttcg
    21241 acgatgaggt aaatgcgcgg cagcagttcc ttgaacatgg ggatagacca tttttccatg
    21301 gcttccggca ggatggtgtg gttcgtatag gccacggtgc ggacggtgat gttccaggcc
    21361 gcatcccacg atatgccttc ttcatccatg aggatgcgca tcagttccgg gatgaccagg
    21421 gccggatggg tgtcgttgat gtggatggcc acatagcggt cgaatttata gatgctttcg
    21481 tgatatttct tcttataatg gcggacgatg ctctggaggc cggccgatac gaagaagtat
    21541 tcctgcatca ggcgcaggcg gcggccgtct tcatggctgt cgtcaggata gaggaaacgg
    21601 gaaatctgct ggacactgtc cttgtagcgg aaggcccggc gccggtcgcc gatggacaat
    21661 tcctggtaga gctgttcccg cgaatattcc gcccgccaca ggcgcagcgt gttgaccgta
    21721 ttgttgtggt agccgatgat aggcacatcg tacggtacgg cgcggacaga cgaatacttt
    21781 tcgtggacac attccaggtt gccgtcttcg tcaggacgca tgtaggcgtt gccgccaaag
    21841 cggacataga cggctttgtc gactttcttg acttcccagg gatagccgtt gaccagccag
    21901 ttatcgggca gttcgacctg ctggttgtcg acaatgcgct ggttgaacaa gccgtactga
    21961 tagcggatgc tgttgccatg gcccggcagc tgcagcgctg ccagggaatc gatgaagcag
    22021 gcagccagcc ggcccagtcc cccattcccc aggcccggat cgcgttcggc cgggatgacc
    22081 ttattcaggt cccagcccat gtcttccagg ccctggcgac agatgtcgtc catatcgatg
    22141 cccaggagat tcgattccag gaggcggccg atgaggaatt cgatggagaa ataatagact
    22201 tgcttaacct tctgttcggc atagatttca ttcgtgcgga tccaatcttc acttaccaga
    22261 tcgcggatca tataggcgat ggtctgatag acttcattca aggtcatgtc ccgcagatct
    22321 ttttcccaca agatgtgagc tttctcggta aataactttt tgaattcttc cttatcctgc
    22381 cattttttca tattgtctct aaccccttca tagttgcgtt tagcggactg aacgtttacc
    22441 aatgcacaac gggttgtcgt ggctgccgct caacacagct tccggctgga tgaccgtatt
    22501 cttgtcgcat acgacgtaat cgagctgagc gtcatcgccg acgaccgagt gctgcatgat
    22561 gatgctgttg cgaatgacag cattttttct gaccttgacg cggcggaaaa gaatcgaatt
    22621 ctctacgcgg ccttcgatga tgcagccatt agctacgaga gaattggaga catgcgattc
    22681 ttccatgtat ttggccggcg cttcgtcctt gattttggta tagatgcggt gatccatatc
    22741 cttgaggagc agttcatgcc agatgcggct gtcgcgcagg tccatgttga cctggaagta
    22801 ggaattgacg gaatcgatcc gttttgcata tccctgataa ttatagccga aaatccgcag
    22861 gcggtctaca ttgcgctgga gcacatcggt aataaaatgg gtatatcccc gggcataggc
    22921 ccgacgtatg caatgctgga atacatcgca gtcgatgagg attccccgct ggtataaatt
    22981 atcgccagct ttgacttccg gcttggcttc gatggccgtg acccggtccg ggcagacatc
    23041 gtcgatggtc aggacatagc cttcgcggtc gaagtcgtat ttctgctgct gatagatgag
    23101 ggtgacgtcg gcgttgtgat gacgatggaa gtggaggacc ttgtcatagt cgatgttctg
    23161 gaccatgtcg cagcccgaca gcagggcgta gcgcttgttg gcccgctggg cgaagatgag
    23221 gttcttataa taagcactga tgtccccttc tacgggatgt tcgatgtccg cccgttcttc
    23281 cggcagatag aacaagccgt cgcccttgtg ggccagaccc cattccttgc cggaccggat
    23341 gtggtccagg accgaccggg actgactgga gagcaggagg ccgacgtttt cgatgccggc
    23401 gttgaccata ttggaaagcg tgaagtcgat gaggcggtat ttgccgccga tgggcagcgt
    23461 cgacagcggg cgcgtgtcgt tgatttcctg caagggatgg tcttcgcgca tattgatgag
    23521 gccgatcata ttactattca taactagtgt tcccctttcg ctgtaacttg ttttggtttc
    23581 agttcccctt tgcgtcccag tacggcgaca gacccgtctt cattgtggag gacggcgccg
    23641 ggatagatgt ggcagcgttc gcccaggatg gccttgtcga cgacggcatt ttcttcgacg
    23701 acagagccgg gcatgatgac ggaattggtg actttcgccc cttctttgat ggtgacaccg
    23761 tagaagatga ctgaattgct gacagtcccg aagatttccg agccttcagc gatgagggaa
    23821 ctcttagccg agccgttagg cccgatgagg tgcggcggta aactctggtt gcccgaatag
    23881 atgcgccact tgctgtcgcg caggtcgata ggcggttcgt cggcgatgag gtccatgttg
    23941 gcctgccaca ggctgtcgat ggtgccgaca tccttccagt agccttcaaa gggataggca
    24001 tacagcggga cctggtcggc cagcatcgcc gggatgacgt tcttgccgaa gtcgtgttcc
    24061 aacttttcat ccttggcatc ggccttgagg tacgattcca ggacgtcgcg gtcgaagatg
    24121 taaatgccca tggacgccag gttgctttcg ggcttggccg gtttttcctg gaacttggtg
    24181 atgcgcatgt tttcattggc gaccatgatg ccgaagcggt tggcttcatc ccaggggacg
    24241 cggatcgtgc cgatggtgac cttggcgtgg tgtttcttgt ggaaattgag catcttgtcg
    24301 tagtccatgg aatagatgtg gtcgcccgac aggatgagga cgtaatcgtc gccgaccatt
    24361 tccacgaagt tcaagttctg gtaaatagca tcggctgtgc cgcggtacca gttggaaccg
    24421 ccgtcgctct gatacggcgg caggacgtac gtgccgccct tggtcgagtc gaggttccaa
    24481 gagctgccgt tgccgatgta ccagttcagt tccagcggct gatactgggt caggatgccg
    24541 accgtatcga tgccggaatt gcggcagtta ctcagtggga agtcgatgat gcgatatttt
    24601 ccgccgaaca aaaggcccgg tttagcctgg tctttggtca ggacgcccaa acggcttcct
    24661 ttcccgccgg ccaaaatcat ggccaggcaa ttagattttt tcatagaagc tcctcctcac
    24721 ttgactggga cccggccgct tcgcttatgg tttcagtatg tatgataatg gacatcgtgt
    24781 cgtcagacgg cgggtcttgg attcaatagc gtaccgtata cgcatataat atacgctata
    24841 aagtatttta tgattatatt attaattata caccaattct gtcaagaatt taaagggcta
    24901 accgccaaaa aatgattagc agaaacaact attttacaca ttacaacaag gactgctatc
    24961 ttttaaaatg taacgttttg aaccggcatg gaattgctct atcgtagggg cgcccacgtg
    25021 gggcgcccgt cagactatgg ggacgacaaa cagcatgacc agtatgagct atgcccggga
    25081 ttcacgcggg ctccccacgt ggaagcccct acaaaaaaag cgctgccgtc aggggcagca
    25141 cttttcacta aatcgtataa ctataaattt tgtaaatttc atacactact tttgttcagc
    25201 ttgcgctaag gcttcctggc ggctcaggat gcgcaccggg atgggcagcg attggagtac
    25261 ctcaatcgtc tcgcctgccg tgtccttcgt cgtccgtacg aagcctatgc ccttcttgcc
    25321 gtccagggcc gtgaccacgc ccaggtagtc acagccttcg atgatgcggt tgatgaaggt
    25381 catttccgac ggaagggcct ggaagtagac ccagtcttca tcaaacatgg ctcttcacct
    25441 ggcggcgcat catggaatat ggttccagcc gttccggtac ggccaggccg acgagctgct
    25501 gggcgtggcg ggcggcgtcg agttcctgcc cgtcttcatc gacgatgcgg tcgatggtat
    25561 agcggaccag atggcccttg ggctggagga attctacggt ctggccgagt ttgaagtggt
    25621 tccgctgttc tacccaggcc aggccctttt cttcatcata ggacttgacg aggccgatga
    25681 aatcatgtgt ctgggtattg ctcgactggc tatagacctg gtcggccgcc gtcggccggg
    25741 agacggagaa gccccgcgtg tagggccgat gggaaatctt ttccaattcg tcgagccatt
    25801 cctggcggac cgtgaagtgt tccgggtcgg cttcgtaggc gtcgaaggcc tggcggtaga
    25861 ccttggtcac ggtggcgacg tagtggatgc tcttcatgcg cccttcgatc ttcaggctcg
    25921 taaagccggc gtcgtagagg tccggcaggt acggcaggag acagaggtcc ttggagttga
    25981 agatatacgt cccccgttca tcttcgacga cggggaaata ctggcctggc cgcttctctt
    26041 cgacgacgtt gtacttgaag cggcagcact ggacacattc gccgcggttc gagtcgcggt
    26101 cgccctgggt gaagtaattg ctcagcaggc agcggccgga ataggaaatg cacatggcgc
    26161 cgtggatgaa gccttccagt tcgatatcgg ggactttggc atggatttcc ttgacgtcgc
    26221 gtaggctcac ttcgcgggcc atgacgacgc gcgatgcgcc gttatcgcgc cagaatttcg
    26281 tcgatgccca gttggtcgtg ttggcctggg tcgatatgtg gacaggcagg tcaggcacga
    26341 gctgccgggc cagggcgaag atgcccgggt cggcgatgag gatggcgtcg acgccgatat
    26401 cgcgcaggta gacgaggtat tccggcaagc ccttgaggtc gtcgttatgc gggaagatgt
    26461 tgaccgtcac gtggacgtgc ttgccccggg catgggcata gtcgacggct tccttcagtt
    26521 cttcattgga aaagttgtcg ctgaaagccc gcaggccgaa ggacttccct cccaggtata
    26581 cggcatcggc gccgtataaa aaggccatct tcatcttatc catactgccg gccggagcca
    26641 gcaattccat ttttcccatt ctcttatcct ttccattgcg aaacggtcat gccgtcgcca
    26701 ttttcaaaaa atacagtctc aaaaagggac cggtcgccga cgaggtccat gaattcccgc
    26761 aaacgggtca cggccgtgcg gtgtttgtgc ggcagggtcc cttcgatata aaacatgtcg
    26821 tggtagtcga tattatccgc tgcgacgatg gcgccaggca ggagcttggg caggagatat
    26881 cgcagctggc gcacatactg ccctttcggc ccgtcgagga agacgaagtc ccacggaccg
    26941 ggaagctgtt ccgccagcgc cgtcccatcg ccgtggatag ccgtaatgac cttttcatag
    27001 ggtgagcgcc ggaagaactg gcaggccagg tcatagcgct cacggtcgag ctcgatggtc
    27061 gtgatcgtcc cgcccggtgc caggaacggt gccatctgca gggccgaata gccgatgcag
    27121 gttccgattt ccaggacatg tttcggccgg cacgacgctg cgatgcccgt gaacaagggc
    27181 agctcgctgt cgcgcaggat gggcaggttc cgctcgcggg cacaggcttt catatcctgg
    27241 aacaatgcct ccatcatggc atgtgctcct ggacgttctt catgtgctcc tcataggtgg
    27301 cggcgaagta attatggccg tccttatcgg cgacgaaata gaggtagttc gtctgtggtg
    27361 cctcgaggat ggcttccatg cagtccatgc cgggattgcc gatcggtccc ggcggcaagc
    27421 cttcatagcg gtacgtattg tacggcgaat cgtactgcgt ctcgttgatg ctgtaggctg
    27481 ccttgtgggt ccccatagca taggaaatgc tggcatcgga ctggagtttc atgtggatct
    27541 tcaggcggtt ctcgaagacc gacgcgatga ggggccggtc cttttcgtat ttcgcttcct
    27601 tttcgatgag cgaggccaag gtgacgaact ggtagatcga caggttctgc ttggcaatgc
    27661 ccgctttcat ggcatccgtc aagtgggcgt cgaagttctt gagcatcatg gccacgacgt
    27721 cgtcggccgt cgcgtcataa gggatgaaat acgtgtccgg gaagaggaag ccttctgtcg
    27781 ggaaggtcac tttgcgattg cccttcatat acggcaggag ctggtccgat gacgaggcag
    27841 ccttgaggaa atcggcttcg ctgatgcgcc cgttggcggc gacggccttg gcgatttcac
    27901 ggaccgtata gccttcgggg acgaccaggc ggtccgcttc cgacttgccg ctggttagtt
    27961 tttccagcag cgtatggagg ctcatgcgcg aatcgaaggt atactcgccg gcatggatgt
    28021 caccggcctg tcccgtgacg gtcgctacgg cgcggaacca caaggggctg gcaatatagc
    28081 cttcatcggt caggacatcg ccgacttccg aagccgtcat gtcgctgcgg acgtggacgt
    28141 agccgctctt atagggcagg aggaaattgg ggccgaaata gccgtaagcc acggcggcga
    28201 tgccgagaca gaccagcccg gctgcggcgg cagcgatacg gcggcccttc tttccaagcg
    28261 gaacgcggcg tttctcatga agttgtgtcg ttgtatgatt ctgttgttcc atggggtatc
    28321 ctatttctgt gcagcccagg tacggctctg ctgggctttt tcttcactgt gattgatggc
    28381 ctgggcctgt ttttcctgtt cgtaacgggc ctgggcagcg gccttggcgg cctggagctg
    28441 ggcctggacg gccgggtcgg cctgcgtgac cgtcccgtcg gcattgacgg tctgggccgc
    28501 atcgagctgg gcctgggcgg ccgagacttc attggccagg ttctggagtt cgcggtcatt
    28561 catattgtgg cgcacgtcgg cttccagggg gacagacggc gtcggcttga catcgacata
    28621 gacttcgaac aggcgctgcc acggcagggt gacatcgacg gtcttggggt cgatgccgag
    28681 atacgtcgac gcatagcgct ggagctgttc cttcgtgcgg ctctggagtt cttcgttgag
    28741 cgtcgggtaa tactgagtga cgacgccggc ttccagtttc tgctgcatac ggtagatgta
    28801 atcgcggaca ttggcctggt acgcccagtt gtcgagatac tgcgtggtca gcatgtcgcg
    28861 gtgggcatcg gccgtcatat acttggacaa gacgccctgg gcgatgccgt agccgacgga
    28921 gttgctggcc gtattccagc cgttgtaggc agccaggcgg tacatcatct tatcctgata
    28981 gaggccgtag accagggtgt tgtcggagcc gttggaaaag gccatatcga cgatgctgac
    29041 cgggatgtcg aggttgacgg ccttttcgat ctgcgtcagg aaatcccgcg tcgactggag
    29101 catgatcggg aagttttcaa agttcgccga ttccgtcgtc gacgtcgtca gcggcgtgtt
    29161 gacagccagc aagaggtccg gccgttcgtt gtcgaccatg gtgccgccga tggcttcgac
    29221 gtgggacgcg atggtcttgc caatggcctg gccgtcatag cggggcacgg tcttttcgcc
    29281 gccgcccaag ggatagatga cggtgatctt gggatgataa ttattgaaat cgttgctggc
    29341 ccgcgtgatg agcagcaggc ccagctggtc ggcaccgggg aaggagccga aaaccgtctt
    29401 ggggatgccc gtaccggcca ttttcaggta cttcgattcc agcgacgact gggtgctgac
    29461 ggaattatcg tcgtgcccca ggctgtaata ggtaaagacg cctttgcggg catcatcgat
    29521 gaggcggtaa ttgatgctca tgttcttgcg gcgccggtta taccagtcct ggaggtattc
    29581 catgggcacg cggcgcatga tggcgaacca gtcattgcgt tcctgcggat tcaggccttc
    29641 ttcgtccatc ttggcctgga gcgatgccag ctgatagatg tcggagccca tggtcaggta
    29701 atagtccggt tcgacgccct tgccgccggc ccagggactg cgcatgaccg tgctgaagac
    29761 ataaatgggc acgttcttgt gcgatttatg cagcgcttcg accttttcca ggcgcgctgt
    29821 cagcgtttcc atcggcaggt tatgcttgcg cgaatcgacg aggccgccat agaccaggga
    29881 atcgatggac aggaccatgg catcggcctt gccggcattg gcattgaccc aggccatgag
    29941 cttatccggc gaaccgtgga agttcatgcc cgacagatac tgggccggcg gcgtcaggac
    30001 ctgatagccc gccgcttcgg ccgtactgac cgtgtattca taatcgacag gccggtcatc
    30061 ctggggtaca tataaaatcg tctttgccag tgcagccggc gaggcacata aaaagaaaga
    30121 ggctacggcc aggctgactg ctttctgtaa cagattcacg ttatcactcc tattatgaag
    30181 aaaaaaattg taccctttat tatatcacgg ccctgggaaa atttgtaggg gcgccctacg
    30241 ccctccgatg gtgggcccgg cgaatccttg ggatattgag gtgcagtcgg atggccacca
    30301 ggcggatggc gacggtgacg gaaaaggcga cgatggcgcc gacttccaag gggacctcaa
    30361 aggggatgac ggcgccgtac aagaagatac tgcccaggag ggacgccgtc gcgtagactt
    30421 ccttgcgcag gacgccgggg atacgcccgg ccaggacgtc gcggatgacg ccgccgccga
    30481 cagctgtcag gacgccgagg gtgatgtcga ggatccagta gnnnnnnnnn nnnnnnnnnn
    30541 nnnnnccgta aaggagccga ggccgatggc gtcgcagacg agataccaga tggagagctt
    30601 gccgacgacg cggcgccaca tgcggatatt gaaatagcgg acgatgatcg tgatgacggc
    30661 catggagccg aggatgatcc acaaatagat actgttctta aaggcagccg gcgggatatt
    30721 gccgatgagg atgtcgcgga tgataccgcc gccgacagcc gtcgccgcac tgaggacgaa
    30781 gatgccgaag aggtccatgc gccgtgaaat ggctgcgaag acgccggata cggcaaaggc
    30841 cgtcgtccct aataagtcga aaatctgcca ccaaatggca tccataatag tttccccttc
    30901 cctgttttcg tgttatgata gacataaatt tttttgctaa ggagactcca tccactatga
    30961 ctaaagatga attttatatg ggcaaagccc tggccgaagc caagacggcc tatgccgtcg
    31021 gagaaatccc catcggcgcc gtcatcgtct atgaaaagaa agtcgtcgcc cgggcctata
    31081 acctgcgcga aagcctgccc tgtgcgacgg cccacgccga actgctggcc attgaaaaag
    31141 cctgccgcgc cctcaatcgc tggcgcctga caggctgtac cctctacgtg accgtcgaac
    31201 cgtgccccat gtgtgccggc gccatcgtca acagccgcct ggaccgcgtc gtctacggct
    31261 gcgacgaccc gaaagccggt gccgtgcgct ccctcttcca gctcgtcgac aacgatcgcc
    31321 tcaaccaccg gaccgaagtc acagccggtg tccgggccga agaatgtgcg gccctgatga
    31381 agagtttctt ccgtcaaagg aggaagaaat cccagagact gttgcattaa gacagcctct
    31441 gggaagtttt aagtttgcag tttgtagtct gtagtgaatg cttttaaatt taaagccatc
    31501 ttctacaaac tctgtactac aaactataaa gactcgctc
//
LOCUS       DAWABZ010000005        30113 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_441_length_30113_cov_3.833988, whole genome shotgun sequence.
ACCESSION   DAWABZ010000005 DAWABZ010000000
VERSION     DAWABZ010000005.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 30113)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 30113)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..30113
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_441_length_30113_cov_3.833988"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         6176 a         8468 c         8686 g         6783 t
ORIGIN      
        1 ttattacaca ttgtggcggt attcataggg gaggactttc aggccgttat agctcgggca
       61 gtcgcggacc cattccaggg attccttgag gatagccgtc tgcatgggga tattgttcgg
      121 ttcgccggca ttggagccga tagggacgtg cggcgctgtg atgcgcggtg ccccctgctg
      181 gcgggcgatg ggaggcaggg cggcgatgac gcagcagctc atgccttcgg cttcgcagca
      241 gcgggtgacg atcgtcgccg aacggtggca ggtcccgcag ccgcccgtgc agagaacgat
      301 gtcgacgccc tgggctttga gcttcttggc aatttccggc cccgtttcat tgcggaattt
      361 ttcgacgttg ccgccgccgc ccatgaaagt atacgtttct tccggcaggg agccgatgaa
      421 gccttctgca accagttcgt gcaggcggtc gatggggaac atggcattga cgtctttgtt
      481 gatatccgag ttgtcgaagc ctccgtgggt gaccatcagg ttcgacgatg gagtgtcgaa
      541 ggggatgacg cggtacgaga aatccccgga cgtattgaac ggcgtctggt cttttttatg
      601 gatgccgcca gccgtgatga aggcgacttt acattcactc agtggtttct ttaattcagt
      661 aaataccggt ttcggtgtaa cgggaacgaa gatttccgag cgcatccctt ctacagtcgt
      721 taagttcata actacccctc ctatagttca tctacagccg ccatggggct gatgtaaaat
      781 tcagtatatt ctccctcttt cagggcgaca tcggtaaaga cccagcgccg cggtacggca
      841 atggtagcca ggccgccgag acagtcggct ttgatggccg tatcgttgac ttcgctgagg
      901 acaccgccgg ccaggaccgg cgtgaattcc cggtcttttt cgagcggccc ataatcgtaa
      961 tcgagcgggg tctctacgat ctgcatgtaa ctaaaataga atcggaattt ccggccttcc
     1021 cggggctggt ggaggaacaa agacggtaca gcgtggatca cgcccagtct ccctctcagg
     1081 ttgagagcca cgtcgtccgc agcggtcttg gtgaccgtcc cttccatgag taccgggttg
     1141 agctgataat cgagtttggc catcagtgga gcgtcccttt atcgaggagg accgtttcag
     1201 ggagaccgag gagtttattg ttggcgttga tgacggcgtt gtcccatttc ttcggtgcag
     1261 ccgcaatggg gacgccggcc atcttattct tgagcatctg gatagcgcgg gctgcgactt
     1321 ctttagtgac gcacgagcag ccggccacgt cgttttcaaa gccgccatgg tccatatttt
     1381 cttcgaccat ggcatcggca tagggattgc cgacgacgag ctggccctgg taggcacaga
     1441 aggaaacgcc gacgacgggg atgccccgct tgccagcctg gccgatgtgc tggatgaagt
     1501 cgatgtggtt attaccaaag ccttcggtcg tgatgatgca gccatcgatg tcgaggcttt
     1561 ccaggagcga gcctaggcgg tcggaaaccc agagtttttc gtcattgacc tgcggcgagc
     1621 cgacgaagac gacgccgatg aggtccagtt catcgtcatt ggccaggcct tcgaccaagg
     1681 gttcacggat gtaatgacgg gtcatttctt tcgtagccgg cccgatgcag gtcagggcat
     1741 ggatggaacc gtcgcggacc tggttcggtg tcagtacaat cggtacgttg ccgaggtcga
     1801 cgttcttctg gccacccatg acgctgcacg gttcgttcgg gcagaggacg ttgtcgtgca
     1861 tagccccctg acccatgatt tccttgacca ggacgacgcg gggattgcct ttgcggcgca
     1921 ggtcttcgca gacttcttcg cggacgactt cgccgtcgta tttcttcaat tcattgcgga
     1981 cggcctggat gatgtagtct tcacatttat gagcggcaaa aggtcccgga cgggtcatgc
     2041 ccgtgaggcg tttgatgacg gcatggcagc ggatgatgat gtcgccttca tcggcgcagc
     2101 ccggatggcc gaaatacatc ttttcatcga gaaagccttc agacgaaccg aattcatgga
     2161 tctggacgcc gtcttcgtcg acgccggtga gcatgaatac gacaccgtcg gcgactttgg
     2221 tgaccccttc accgagttcg ccttcgactt tcgtcgcaat gggcgctacg tccatgatgg
     2281 tatcggtgta gacgtggcgt ttatcgggat aaatgacgtc gaggctcatg ctgacgcaga
     2341 gcgggtcctc tttgacggcc ttttcgacga tggacccgtc gatgaagatc ttcccgtctt
     2401 caatggccgt cttgtcgctg atttcgacat cggtgatttt gatgtgtttc ttgatgagct
     2461 tgcggatgac cttcttttcg cccgtctttt ccgtcggccc tgccggcagc gggccagcgg
     2521 cacccaggat ggtaccggcc gggatatcca ggctcaggcc ttccagttta tctatcttca
     2581 agtgaatcgt tcccgacggt gcggctgtcg gggcggccgg tgcagctggc gaggctttga
     2641 ccggttctgc cggtgcttcg gccgggacgg acttggcatt gactttatcc aggacatctt
     2701 tagtaatcgg cgtcaaggct tcgacatcct gcaagagcgt gctgcccagg acttcttcga
     2761 tggtcaggcc atcgtcactg agcgtgagca ggccggcatc gaccatgtca tcgaacagcg
     2821 tcgggtcttc caggtcatcg gctccgatga ccgtcccctt ggcccggcgg cagcagaaaa
     2881 cggcaggatc tgtaaggtgt ttctgtaagg tatctttggt aatagacata caatcactcc
     2941 ccctgtgtag tatgagttac gccttgatgc ggcggattaa tttatgatgg actgcccgca
     3001 gggcgccatc ggtacaatgg tagaccggca gatgaagctg cggcgcacag gccatgaccg
     3061 tgttggtgca gtctgtgcag ttactgatga gtagggcctg ggccccttct ttcttcagcg
     3121 tcatgacccg ctggacctgt cccggacagt ggccgggatt gtcgcatttg cggacgcccg
     3181 gtttgacttc atgggcctgc ttgcaaaagg ctacgccggc tacggccgac cccatggaag
     3241 ccgctatttc ccggaggcgc ttttcatcgg cgccgcaggc acaaacgaga aggccgattt
     3301 tttcaggccc tggcaggaac gtttctgccg cgatgatgcc gcctgtcgtc ttgacgatgg
     3361 gtgcgtccag gctcgtccgc ctgcccatga aaggcccgcc catgatgagc tcgccatatt
     3421 ctttgcccag gccgccggcc ttttccagga catcgttgac ggccatgccc aagggcaggt
     3481 cgtggaagac ctggacttcc ggaccgttca gcttgccagc tacggtcacg tctttatcga
     3541 tgagcggttt cttgtcatcg acggcttcgg ccacgcgctg gagcgtttcc acgttgatga
     3601 tgacggcgcc ggctttcgac ggcagactgc ccgcatcgag gagcgtcccc aggacttccc
     3661 ggacgacagc ccgttcttcg ccgacaggat agacattggg cagaaaatga agggccaggc
     3721 ggtcgtcagc ggcgatggcc tgctgtaagg ctcggacggc gtcgccgtgg atggccttga
     3781 tggcaatgac gcctttatcg gcatggacga ggtccatggc cagctgcagg ccccgtatca
     3841 tcgggcggca gtcccgctca atggccttga tattatgggt caggacgggt tcacattccg
     3901 aagcattggc aatgacgacg ccgccgttca aaaagggaac ggccagtttc tgatacgtcg
     3961 ggaatccggc gccgcctaag ccgacgatcc cggcctggcg gatataatcc aatggagtct
     4021 gtgccgtaag aggcacgtac tctttcggct ggacgtcgtc agctcgaacg atgacggcat
     4081 catccgtcac ttccaggacc gtcccggata cactcgtata taccgggctt cctaatcctt
     4141 ccccagcctc agcgatgcac tggccgcgct gtaccctgtc tccaggctgg accactggga
     4201 ccgatggagc gccgatatgc tgtgaaagag gaaaaacaaa gggtctcacg tccgtccacc
     4261 tcctataatc atgatgtcta aattcttgct ccgcttgatg tcctcaagca tggccttagt
     4321 atagtcccag cctatcttga aaaaaacagg aaaatttgaa attttcatga attcatcatt
     4381 tattccttaa atttatttgt tctttatggc atcgttttgt gttaaaataa gataaatagc
     4441 cgtctgaggg gatatattaa aagatggctg ccatatagag ggggaatgtg catgattgta
     4501 gaaacctacc cgattgcttt gaaaaaattc atgaaattca cgaaaatgaa gtccgtgacc
     4561 ctggccgaat acatcggtta cgacgtatcg tatatcagca agtggatcaa cggggccaag
     4621 ctgccatcgt cacgggccat tgaacgcgtc aatgaagatg cggctgcact ctttgccgaa
     4681 acgattgaaa aactcgatcg ccgccaagac tgctgccgct atttttcgtt tcccgccgat
     4741 acgcccgacc tggtctttgc catcagcgac tatctgagcc gggcttaccg cacgtcgctc
     4801 cagagcgggc gcacggaaaa ggccaaaaag tcctggcccg tccgcatcat cacgagccat
     4861 cagaaggtag cccacttttt aaaagactta ttccgaaaaa aactcccgac cctgacgggc
     4921 aaggaggaac tcgtcatcta cggggaattc ttcgattttt acgacgacgg cttctggtcc
     4981 tacctcgacg tgccgtccca ttataaacgc ctgcgcatcc gcgtcggcct gaataaagag
     5041 aagatacgcc gccatccgga atacgtagcc cggctctacg ccgtcctcaa ccgcttcatg
     5101 gatgcggaat tcacctttta cgacttctcc gccttgagcg atgccaatct gatcatcctc
     5161 aaggaccagt tcgtcatcca gtattcgctc cagagccatc ggggcatgat gatgtgttcc
     5221 tacatcgacg acccggccgt cgtccacgac atttacgagc gcttcggcgt ccaccatacg
     5281 ccgaaactcc taactcacat ccagagtccc tggaacgacg actacggcta ccgcacgtcc
     5341 ttctatgaaa cgtcgcggtt ctgctttttc ctgaccaacg gcatcgaata cctcctgccc
     5401 caggaagtct tcgaccggct catcgccgaa tcgccggctg accgggccgg caatatcgaa
     5461 cgcctgcgca ttacctggga agaaatcatc gccaaggccc gcgtatcgct catcgtaccg
     5521 acgccgtcct tcatgcgcta tctggaaacg ggccacattg acctgaccga cgtccagtac
     5581 accttgacgc caggcgaacg aatagaccac ttgaagacca tcgcccggac tctgacccag
     5641 agccagggca tcatcttggg gaccatgcac ttgtcgccgg ccttcctggc ctataaagaa
     5701 gcgaacctgt ccttctattc caactaccgc atgagcttct tcaaaaagaa caccctggcc
     5761 atgaacagcc ggaccgaccc gttctacatc atcgtcgata aggacctgga cgccatggtc
     5821 ctggacttct tcgacaacat gacgaaatcc acctactgcc atccgtgcag tggccaggag
     5881 ctggaaggaa aaatcaaggt ctaccaacac atgctggaaa aaatcaccag cttggctggg
     5941 acatagaaaa aaggcttgtc gccgtgcgac aagccttttt aagttgtagg ggtcaccacg
     6001 tgggcgaccc gccggggatt cggtaactat cctggacggg cttgccatgt gcaagcccct
     6061 acggactaca aactattttt tacatggaaa tcttgctggt cttagtccag atacccatct
     6121 tttctgcgtc tttgatgagt tcttcacgga agtccggatg agcgatgttg atcagggctt
     6181 cggcgcgttc ccacgtcgat ttgcctttca gcagggctgt accgtattca gtgacgatgt
     6241 agtggacagc cgaacgcggt gtcgtgatga ccgtgccagc cgggagggtc ggtttgatca
     6301 gggattcgac atggccgtcc ttgaatttac gcgtcgaatg gacgcagatg aagctcttgc
     6361 cgccgttgga tgcgaatgcg ccctgggcga agtcgagctg gccgccggtg ccgctgatct
     6421 gctgatagcc agccgtttcc gcgttgacct ggccatagag gtcgaggtcg atgcagccgt
     6481 tgatggagat gaatttatcg atgctggcga caacgccgac gttgttgacg tagcttaccg
     6541 gagcgttgaa gacgatctgg ttgtcgttga tgaaatcata gaggcgctgt gtgccgccgg
     6601 caaaagcata gatgatcttg cctttatcgc ggttcttgtt gcccgtaatc ttgccggctt
     6661 catagaggtc gacataggaa tcgacgagca tttccgtatg accgctcaaa tctttgacat
     6721 ccgattcagc cagtttgcag ccgacagccg acgggatacc gccgatgccg agctggagcg
     6781 tactgccgtc ttcgatcatg gggatgatgt aatcggcaat cttgtcatcg acttccgtac
     6841 cggcgcggtt ggggatctgc ggcatgggat ggttcgagcc ttcgacgaca tagtcgacgt
     6901 cgcagaggtt cagttccgtt tcatagccca gagctttcgg catgttctgg ttgacttcga
     6961 ggatgatcgt cttagcagca cggatcaaac cgcgaaggtc cgatgcctga ggcccgaggt
     7021 tgaagttgcc ccactggtcc ataggatgaa cctggatgat gagtttatcg atgttttccc
     7081 aatacttcgg caattcgttg aagagaatag gaatgaacca gcagcggccg gctttggaca
     7141 tcttgcgttc aaacccgttc atatgagccg ttgcgaagcg gatctgttcg ttcgagtcac
     7201 tgttgagata agccgcatag ggttcatctt tgacgacggt cgtggcaacg atttcgacgc
     7261 catgcaggtc tttcgcccgt ttaccgattt cgatgtcgat atcatacgga gcagcgcagc
     7321 cgaggccgta ggaaacgcgg tcgccatctt tgacgagagc cgcagcctgt tcagccgtga
     7381 tcaatttctt cttatactct tcaggaattt gtgaaagttt gtacattcta aagtctcctc
     7441 cttcatgact ttgcaatagt agtatgagat aaccttatat atagtataaa actttcccgc
     7501 ggcgactgca atcatatttt gtgaaaaata ttacaaaata tgctattata ccagaatagt
     7561 ccgtgatgaa atctccctaa cgggccgcct tttaggggga cagcccttac gcacaaggcc
     7621 acaggccgtc tatttacgac cggccacttg ccacctgcgg cctgcattta ttttttcatc
     7681 gttattcttt tcgtcggcct tgacatccgc cgtgttgacg gcttctttga attcccgcag
     7741 gctctttccc agggcaatct atttttgttt acattttctt caaggcagta taaaatgaaa
     7801 atattctggt tatttttcgt ttagagaaag gattatgcta tgtatcattc acaagctaaa
     7861 ggcacgatac tgctgctcat tacggccctc atctggggcg ctgccttcgt cgcccagagc
     7921 gttggcatgg actatatcgg cccgtttacc ttcagcgccg cccgcgatgt catcgccatc
     7981 atcgtcctga ttcccgtcat cctgttgttt accgataaag ggacagacgg gacctatccg
     8041 ccgattttgc aacagctgaa accggaccgc atcaccctca tcggcggtgc ctggtgcggc
     8101 ctggtcctgg gcgccgccga tacgctgcag caggtcggca tctccatgac gacggctggg
     8161 aaagccggtt tcatcacggc tttgtacatc atcctcgtcc ccctcatggg ccatttcatg
     8221 ggccacaaag tcccgcggat catcgtaatc tgcgtcaccc tggccattgc cggcttttat
     8281 ctcttatgca tcaacggcga tttccaggtt tctttcggcg acttcctggt cctctgctgt
     8341 gccgtcttct tcgccctgca catcctggtc atcgaccatt tcctcttgaa aaaagccaac
     8401 agcatcaagt tgtcctgggt ccagttcgcc acggccttcc tcttttccgg caccttgaca
     8461 gtcctcttcg aacagcccga ctggtccgcc ctgtgggccg ccaaatggcc cctgctctac
     8521 gccggcggcc tgtccagcgg tgtcgcctac accctgcaga tcgtcggcca gaaatatacc
     8581 gaaccgacga cggcgaccct catcatgagc ctggaatcgg tcttcgccgc cctggcaggc
     8641 tggctcatcc tgggagaagt catgacggcc aaagaactga caggctgcgt cctcgtcttc
     8701 gccgccgtca tcttagcaca gattcccctg ccacatctct ctttccgtca aaagaattaa
     8761 attatatttg aaaatattat atttcaaaat ataatatttt gaagtataat atttttcttc
     8821 taaattctca aaaaaaagct gcctatgtcg attgacataa gcagcagcga aaaaaattca
     8881 ttaaattttg ttgctccgcc gccagatgcg ctgggcttcg gcggctttga tcaagtcttc
     8941 acgcatatcg ggatgggcaa tgtttatcag ggcttctgcc cgctgccacg tggccaggcc
     9001 ggacaggttg gctacgccgt attccgtgac catgtatggc gcctgggtcc gcggcgtcgt
     9061 gatgatatcg ccggcagtga acttggggag gatgttggaa tggcggactc cctttttatc
     9121 gacccggctc gacggcatgg ccaggaaggc tttgccgtgg tcggcggcat aggcaccggt
     9181 cacgaagtcc aactggccgc cagtcccgct gatgtgccgc gtaccggccg attcggagca
     9241 gacctggccg taaaggtcgg cggcgataca gctgttgatg gaaatgaaat catcgagctg
     9301 gcggatggtt tccggattgt tgacgtaatg cataggcgct gacaggacat cctgattgtg
     9361 gtccaaaaat tcatagagtt ccttcgagcc catacaaatg gagaagacgc ctttcccccg
     9421 ctggaaaggc tttttgtcgt tggtaatatt gcctgcctta tacaggttga gatagccgtc
     9481 gctcatgagc tctgtatgca tgcctaagtc cttcaagtcc gattcggcaa tgaggatacc
     9541 cagggcattg ggcatgccgc cgataccgag ctgcagggta atgccgtcgt gcaggtaggg
     9601 gaaaatattc tcggcgatct gcttgtcgat ggcactcggt tcccgattcg gcgccaggct
     9661 gaccgcttcc tggctttcga cgatggcctg gactttcgaa acgtggatgt ggtcgctgcc
     9721 catgccaaag acgacgggca tgttgggatt gacttcgacg atgatcgttt ccgccgcgtc
     9781 gagcatttcc tgggtacagc cattggtcag gccaaaggaa aaattcccgt gcctgtccat
     9841 gggagctacg gtgatcatgg cgacgttaac cggcgcaaag ccccgcgtat aataggagcc
     9901 gcagtcgcgg aacagcatcg gctcatggta ggcccggccg gcatcgagat atttccggtc
     9961 gatagcggaa caatgccaga gattatacgt aaaggtctcg ttttccgggt cggcttccaa
    10021 aatagctaca gggtggcagg aaatggcatt gcggaccttg atgtccttca aatccccctg
    10081 gcgccgggcc agggccttgt ccagggccgt cggataagca cagctcatgc cataatcgac
    10141 ccaatcgcca tccttgacga cctgcacggc ctggtcagcc gtacagcatt tccctttata
    10201 ttcctgatat acgtccatga ccggtcacct cactgtctgg ccgcagccat accggcgtca
    10261 aaggctttta agttgatgtc gacaaaggct ggtttcactg ttttggcaat gatgctcttc
    10321 caatcgatat catggaggcc cagggcttcg atgagggcgc ccaggaggac gacgttggca
    10381 ctcttgggat tgcccaggtc ggtggcgatt ttcgtcgcat cgaggtcccg gacggggatg
    10441 tgtttcttca gttctgcaat gatgccctgg ggatactggc aggctccgga aaggacgggc
    10501 agggacggga tctgcgtcgt attgacgacc atcttgccgc ctttcttgag gaagcgggca
    10561 tagcgggcgg cttccatttc ttcaaaggaa acgaggatat ccgctgagcc ttcgccgatg
    10621 atgggcgaat agactttttt gccgaaacgg acctgcgtgc tgatgctgcc gccgcgctgg
    10681 ctcatgccgt gaacttcgga catcttgaca tcgtacccgt gttcgataag gccgatggtg
    10741 aggatcttac tggctaatat cgtgccctgg ccgccgacac cgacgaagag tacactcgtt
    10801 acatctgaca tattatttat ccccctttct ggaaatggcc tgaaccgggc agacctgcat
    10861 acagaccgta cagccgacgc agtcggcctg ggcaatactg gatttcttgt tttcactgtc
    10921 atagcgcagg gccgggcagc cggtggaaag gcatttctta cagccaatgc acgtatccct
    10981 atcgacgacg caaggcgtct tgtcgaggtt gaactgggct ttatcttctt tcgagaactt
    11041 cttgaggacg cagggccact tggtgatgat gaccgacggt tcctcttcga tggcaaaggc
    11101 ccagtccaac gtttctttca tggccttcag gtccagggga ttgacggtac ggacgttctt
    11161 gatacccaag gctttgacga ccgtttcgac atcgatgagc gtcgccggtt cccctttgat
    11221 gttgtagccc gtcccggggt tgtcctgatg gccggtcatg ccggtaatgc ggttgtccag
    11281 gatgacgcag atggcgttgg agccgttgta ggccacttcg gtcagggagt tgatgcccgt
    11341 atggaagaac gtcgaatcgc ccatgcaggc gacaacccgc ttcttctgat cactgagctg
    11401 gaggccatgg ctgaagccgt gagcaacgga gaaagcactg cccatacaga cggccaggtc
    11461 cttggcgttg agcggcgccg acccgcctaa ggcatagcag ccgatgtcgc cgatcatgac
    11521 cgtatctttc cgcttgccca gttcatagaa gaaaccgcgg tgcggacagc cggcacagag
    11581 atcaggcggt ctgggaacga aacaggaatc ggagacgtcc acgccgtgct gctctttctt
    11641 gaggacgccg gccgatacga tattggggtt cagctcgtcg atcttgggga tgacatcctt
    11701 gccgtggcag ggaatcccgg cctggcgtat ctgttcttcc atgaagggat ccagttcttc
    11761 gatgacgtag agttcgtcga ccttatcggc aaaggctttg attttctgca tcggcagggg
    11821 ataggtaaat cccagcttca gatatgaagc ctggtcgccc aagacttctt tggcatacat
    11881 ataggctgcc ccagcggcga cgatgccgat tttcgtatca tgcatctcta cttcgttgaa
    11941 gggacagatt tcggaataac ctttcaattt ttcttcccgt tcttcgacga catagcggcg
    12001 tttcttggaa atggccggta cggcatccat tttttcccgc tgtttctgat agaggacggg
    12061 cggcacttcc tggcggtcct gttcttcgac gagactcttg gaatggcaga cgcgggtcgt
    12121 catgcgtatc atgaccggga cgccataggt ttcgctgact ttataggctt ctttggtcat
    12181 gtcgatacat tcctgcgaat ccgacggctc gaacatggcg attttggcaa acttggcata
    12241 attgcggtta tcctgttcat tctgggatga atgcatcccc gggtcgtcag ctgaaacgaa
    12301 ggcaaagccg ccgttgatgc ctaaataagc aaaggtgaac agcgggtcgg ctgcgacgtt
    12361 gacgccgacc atcttcatgg ctgcaaaaga gcggacgccg gacatagagg cgccgatggc
    12421 cgcttccatg gccacttttt catttggtgc ccattcggca tacatatcat catatttcgc
    12481 gacattttcc aaaatttccg tactcggtgt tcccggataa gccgaagcaa aggacacgcc
    12541 ggcttcatac aagcctctgg cgatggcttc atcaccggtc aataactttt tcatactcca
    12601 tacctcctcc atcgttcgat cggttcatta tctattcact aatctatatg taatatccat
    12661 gccaattcat tccatgaggt atataagcca ttttcccgaa gagggccttc gacgataggc
    12721 tcattaggta aaatactttt taccttcttt acatttattt cgtatttctt tatgatggaa
    12781 agagaaacgg aggaggaaag aaaatggata ccatcgcttt tttgattgct gacgagttga
    12841 tgcagcagtc tgtctatacg atactgcaca tttataaaga taaactggaa gcccatgaca
    12901 tcacgacgac agtcacgatc atcgacttcc cccatctcat cgaccaggcc cgcggcctga
    12961 tacgccaggg cgccaaagtc atcatcacca acagcggttc ccaccagata ctgaccaagg
    13021 ccatcgacaa cgtgccgatt ctctgccttt tcagttcgac caacgacgcc ttatatacac
    13081 tgcgctctat tgctccgaaa tacaagcaga tccacttgct gctcaatgag aacttcatgt
    13141 tcaacaaaga tgcctgtccg gaagagcttc gcaaaaaaat cgtcttctat ccccgctaca
    13201 gcctggataa gacgcactta gagctgttag cgcgggtccg tcagcttccg gtcacgccgg
    13261 atacggccat cgtcggctgt cccctgctgc cccagattgc ctatcccagt cccatgccga
    13321 tttattccat caggcccagc gaatcgacga tgctctccgt cctgctctac gcccaggagc
    13381 tcctggtctt tcaaaggaag gacaataagc agctgtccat gatgtcgtcc atcttatccc
    13441 acgtcacaga cggcatcatc ctctatgcca agggcggcgc catttcccac atcaatgaac
    13501 gggctgcgtc cttcctgggg attccggtga cgacgaagaa catgcgcgac atctttcccg
    13561 actggaccga agggtcgagc ccgtctttcc acgagacgat tctccagcgg ccgccgtata
    13621 cgctcatcgc caattctgac tttttcctca tggacagtag gtcccaatac atcctgacta
    13681 tccgcgacgt gacggaactg cagcgcctgg aaaagaatat ccgctacaaa ctctccaaga
    13741 cgggactgac ggcgacgcac catttcgagg acatcaagac cgtcgacgaa ggcatgaagc
    13801 acgtcatccg ccaggccgaa gtcatggccc ggtacaacgc gcccatcctc atccagggcg
    13861 agagcggcac ggggaaggaa ctctttgccc agagcatcca caatgccagc gaccgccgtc
    13921 acggtccttt cgtggccatc aactgcgccg ccctgccgac agacctcctg gaaagcgagc
    13981 tcttcggcta tgttggcggg tcctttacgg gcgcccgcaa agaaggcaag gccggcctct
    14041 ttgaaatggc ccacaacggg acgatattcc tcgatgaaat caacagcatg gcgccgagca
    14101 tccagtcgaa gctcctgcgc gtcctggaaa cgaagcaggt catgcgcctc ggttcggact
    14161 acgtcatccc cctggatatc cgcatcatct cggccagcaa tgccgacatc atcgccgctg
    14221 tccaggccgg cagcttccgg cgcgacctgt tcttccgcat caacaccctg cgcctgagcc
    14281 tgccgccgct gaacgaacgg cctgacgaca tcctctacct cttcgcttac ttcctggaaa
    14341 atttcaccca tcgccggcag cccctcaagg cgtcattaaa aaaagccctc atccagcatg
    14401 actggtgggg caatatccgc gagctgcaca gcgtcgcctt gcgctattat atttttggcg
    14461 atactctgga cgatgactac gataccttat tcgatatccc ggacgaggcc caaaagaacg
    14521 ccctgctgga tacggcgtcc ctccagctga acatgaagag cctggaaaag acgatccagc
    14581 agattctcat tgaagaactg caagaaaaag gctatagcca gaaggatata gccaaattgc
    14641 tcaacgtatc gcggcagacg attttcaata agatgaggcc ttagccttta cggcctccag
    14701 cggcgccgga tgcggctgtt ggggatgttc aatttttgcc ggaacttggt caccgtccgt
    14761 cgggctacgg aaatctgggc agaggcaaaa tattcggcca agtcctggtc cgaatagggg
    14821 tggtccttat cttcgctttc gacgagatcc gcgattttcc gcatgatagc tttatcggat
    14881 acgctggccc catcttcttt atcgacggca taggccgtag ccaaaaagga ctgcagtgga
    14941 tatacctggc cttcaaagag gacgtagcgg tggcggcaga cgcggctgac cgtagccgtg
    15001 ctgagtttcg tctcctcggc gatgtctttc tgccgcaagg gctgcaaggg cttatggtac
    15061 aagaaatagt ctttctggtg gctcatcagg caggtcacga cttgttcgat ggaatgatgg
    15121 cgataggcca gggccgaggc caggtcattg aaatcacggc gggctttgcg gatatagacc
    15181 agggtctcct tgtcgccctg ggcggcgtac tggtcataca agtcatcgcg aaaatagaca
    15241 tccggtatct cttccaacga gcgcagggcc agctggcctt tctcgtcgca ataaatctcc
    15301 acgtccggcc ggatatagac ttcttcctgg gtaatctggc cggccgactg gagggacagg
    15361 gtcttgagga aatcgcggat agcctgcagg cccgcttcag acagggccag ggaagcctgg
    15421 attttctgcc atttcccctg gaggaagtct tcataatgct gcgccagcag ggcttccgtc
    15481 ttggcagggg ccttgcggct gcgccgggtc tggatgagca gggcttcgcg cagatccctg
    15541 gcggcaatgc ccggcgggtc aaaggactgg acgagttcca gccctttttt catgtcttcc
    15601 ggagtgagat gataagccgt gcccagggaa gccaggtcgc cctggaaaaa gcccttatcg
    15661 tcgagggagc cgatgaccag gcctgccgcc atcagctgcg gcctgggcag ggactggacg
    15721 cggagctggg ccatgagccg ggcttccagg gaatcactgc ggctgcggag attgtcgatg
    15781 gccttatcgc ccttatcgcc ggccggccgg acatcgtgat agcggatatc cagcaaggga
    15841 ttgtccgtca cctgttcctg caaaaaatcg gccagatcct gggcgttgag ggccagcata
    15901 tggatggtca ggcgctggac ctgcaatatt ttcgctttct gttcgagttt caacgataag
    15961 ttcgacatga cacgcctctt tcacgtaaag aattattttt tcaccatagc ctctaagata
    16021 cgatgggctt cgtccggttc gtggtctttg atgatgcggc tcaccgtatc atatcccagg
    16081 gccccgatat aggctgcgcc aaaggcagcc gaagcctgga ggtgctgccg gcacgagtct
    16141 tcccggactt ccagtccttc gacacaggcc gtctggaaga tgcgcactga ccggatgagg
    16201 agctgcaggc tttccagcag gcagtcggcg atgagcggtt caaaggcatt gagttcaaat
    16261 tcgccgtgag cggcggccat ggcaatggcg ctgtcgtcag ccatgacttt catggcgcac
    16321 tgcatgacca tttccgggat gaccggattg accttgcccg gcatgatgga actgccctgc
    16381 tgacgggcag gcaggcggat ttcgccgatg ccgccatgag ggccggaatt catgaggcgc
    16441 aagtcgttgg cgattttcat gagattgacg gccaaggcct tgagcaggcc cgacacttcg
    16501 acgaagacgt cgttgttctg ggtcaggtcg acgggatatt ctgccgcggc caggccgatg
    16561 cccgtcagct ggcgcagctg ttcgatgact tcgtagcggt actgccgcga cgccgtatcg
    16621 gccgtgccga tagccgtgcc gccgaggttg accatgcgca ggcgttcctc gattttatag
    16681 aggcgccagc ggtcacggga cagggcctgg gcataggcgc cgaactcttc gcctgccgtc
    16741 atggggacgg catccatgag ctctgtccgg cccagtttat atgtcttctc ataggcctgt
    16801 tctttgtgct ggaaggcttc ctgcaagtcg gcacaggcct tgctcagctc ccgcagctgg
    16861 cggatggcgg caatgcgcag ggccgtcgga tagacgtcgt tcgtcgactg gccgcggttg
    16921 acgtcatcca ggggatggat gacgtcgtat tggcccggct ttttccccaa tatgaccaag
    16981 gcggcgttgg cgatgacttc gttgacgttc atgttcgtcg acgtaccggc gccgccctgc
    17041 agggcctcgg tcacaaaagc gtcgtcgtac tgaccggcca ggatggcatc acaggcagcg
    17101 gcaatggcct ggtaaatccc gtcttcgcgc acacccaggc gggcgtaggt cagggcggcg
    17161 gccttcttga ccgtcaccat ggcatagatg agcggcatag ccgtccgctt gtggcctaag
    17221 gggaaatttt ccaaagccct ggccgtgttg ataccgtaca tcgcattttc cggcaaatcc
    17281 acttcaccta gtacatcgcg ttcgatacgc gtgtgttcca tccgaatccc tcccaaagta
    17341 atcgccccta caaactacaa actacaaact acaaactaca aactacaaac tacaaactac
    17401 aaactatttt acaccagcga ctgcaaatac gggaaaacgc tgaggctgcg tttcaagatg
    17461 ccctgcatat aggcgatggc gatgccgtaa ttggtaatcg gcacctgctg gtcggcagcg
    17521 catttcatgc ggtactggac ttcccgcccg ttgagcatac agccgccgca gtggatgacc
    17581 agggcatagc ccgataaatc gtcggggaaa tcgcggccgc tggcggtttc gatgcggata
    17641 tccttctgcg tatagttgcg cagccagcgg ggaatcttga ccatgccgat gtcgtcgcat
    17701 tggcggtggt gcgtacagcc ttcagcaatg aggaccgtat cgccgtcgtg cagtttttcg
    17761 atggccgtga cgccgcggac ggccgtttcc agcaggccct tgtgccgggc catgaggatg
    17821 gagaacgacg tcaggcgcac atccagcggc gtgtcggccg agactttggc aaagacctgg
    17881 ctgtccgtaa tgaccaaggc cggtttctgg cccaggcagt ccaaggtatg gcgcagttca
    17941 tattctttga cgacgatggc ggcgctgtcg ttgtcgatga tttcacggat tgtctgctgc
    18001 tggggcagga tgaggcgccc tttcggcgcg gccgtgtcga tgggaacgac gaggacgacg
    18061 aagtcgccgc cctggatgag gtcgctgacg atgggcgcgg ctgtctcaac gggcttcagg
    18121 cgggccaggc gttctttcag ttcatggatg ccctcccctt tcagggcact gacgtagatt
    18181 tcatggtcct gcgcctgtcc gggccgctcc agcagatcgg ctttattata gacgacgaga
    18241 taaggcaggt tcttttcccg gatgagattc aggacttcgt catcggccgg cgtccgccct
    18301 tcggcagcgt cgatgatgat gacggctata tccgtcttgt tgaggacctg ttttgtccgc
    18361 cgcacccgtt cttcacccag ggcgccgacg tcgtcgaaac ccggcgtatc gatgatgacg
    18421 actggcccta agggcaggag ttccatggct ttatagaccg ggtccgtcgt cgtccctttc
    18481 gtcgccgaca cgatggacag gtcctggccg gtcacggcgt tgacgacgct cgatttgccg
    18541 gcattgcggc ggccaaagaa gccgatatgg acccgttcgc ctgacggcgt atcattcatc
    18601 cccatagata tcgctccttt cctaaacctg gagcagcttt tgagctaact cccgataaat
    18661 ctggctgacc ggcaggtccg ggtcgccttc gacgacggtc atgcctttat tttcataata
    18721 ctggatttcc ttgcttctgg gaatatcggc tacgatgggc aggccgtgtt catcggcaaa
    18781 ggccttgacc agttcgtatt catcatcgac attgcgccgg ttgaggatga tgcccttgac
    18841 ctgggcatag ccgcggctct tgaagttttc gacggccttg atgatattag ccgctgcgta
    18901 gagggccatc ttttcgccgg acgtgacgat gaagatttca tcggcatagc catcgcggat
    18961 gggcgcagag aagccgccgc agacgacgtc gcccaagaca tcatataaga cgacgtcagg
    19021 ctggagcttc tggaagactt cctgttcgtc catgaggtcg aaagtggcga tgatgccccg
    19081 gccggcacag cccaggcccg gcgtagggcc gcccgtttcg atgcagaaga cgccgccgaa
    19141 gccttcccgg ccgatatggt cgaaatccgg tccttctgta tgttcccgat agtagtccat
    19201 gaccggcgtg acgggatggc cgtgcaggag gttcagcgtc gaatcggcct tagggtcaca
    19261 gccgacttgg acgacttttt tccccatcat ggccagggcg gccgacacgt tggctgccgt
    19321 cgtcgacttg ccgatgccgc ctttcccata aattgcaaat tttctcataa gacttcctcc
    19381 tcacgtcaat ttatttttca gccatgccga aaaggacggc ccgacgaaga tgggcatatc
    19441 ggcgtgataa tgccgtcccg aataggcttc atggggcatg gaaatgaatt tttctttttc
    19501 atccggcagc agccgggcgt aaatcgggtc ggcaatgacc cggcgggctt tgcggcattc
    19561 ctgacggatg acatcttcga cgctggccac ttcgacctgt tccaggagcc accgcggcgc
    19621 atcggcaagg gggcagagca tgcggacgtc ggaaatgccc atttcctgct ggagcatagc
    19681 cttcatcgac aagaggtaga ccggttcgcc gatgaccagg acgtcacagg gcgtatcggc
    19741 gtagaccggc tccgtcttcc aggcgttgag gtcggccagg gcatactggg ctttgatgta
    19801 cttttcctgt cccgtaaagc ctgtgagata cgacgaatcg cagttccgca aggcttccag
    19861 ccagtcagcc gtcccctttt cacccatggg gatgccgacg acataaggga tgccgtaggt
    19921 atcgcgcatg tactgggcca ggtagaaggc cgtcgacgac aggacagcgt tgacgtcggc
    19981 attggccgct tcggccaacg tgtccagggt atcgcccatg gaccacgaac tctgcagggt
    20041 aaagccggca tcggtgacga tttgcttgag cgtcgtcgca ttgccgacga cggaaaagtc
    20101 cagcggcgtc aggcccagga gattgacccg tttcgccggt ttctgcggct tgcgatgagg
    20161 cgccttcaaa aaggtcttgg ccagccagac ataggcgccg ccggcaccgg gcaggtaact
    20221 gtggataccg tcggtccgga agcccagcgt cggcaggccc gtccgggccg ctaccaggcg
    20281 ctcgatggcc ttgaagtccg tgccgatggc attcggcatg ggactgccgc cgatggcgac
    20341 aaagcgggga tgggccgctt ctacggcttc ggcgatattg tcgatgaggc gctggtcatt
    20401 gccctggatg gtatcgattt cattgatgcc cgatacgtag accatgctcg gcgtatcgta
    20461 ccaccgcggt tcatcgtgcg tatcgtatgt cgaattacag ccgtcagcat cgtgcatgac
    20521 gatgaggccg cctaattcaa agagcgacga tgcaatgccg aacatgtcga cggcatacgt
    20581 cgataagacg cgttgtgctt gtttcaacca tgacaccccc atgcttttat ttgaatgata
    20641 tcccgcatct tgggattttc ggcggcattg gcctggccga tcaaatctaa aatcttcaac
    20701 aggcccgtat agccatagaa accgccgttt tcaacgagat tgacgaaata cttcgtatcc
    20761 gtgaaatagg ctgccatctg gccgatggcc aggactttcc cgtacttctt actgtcgtca
    20821 cgcggcagga ggcggcgctt gaaatgcgac gtcgaacgca gggacaggtt cggatactgt
    20881 tcccgcagga actggaacgt ttccttttca tcaggcgaca tttcgtcgag ataaatggcg
    20941 tagacgttga aaccgtgggt caagaggaac gcagccagtt ccaagggccg cggcgtcgcc
    21001 gtatagtcga tggcgatgtg cgtatcaccg acttgttccc gcagggtttg gactttttct
    21061 tcgatagcct gttcataggc gtcgcaatcg ggcatggtca ggcccagggc atccgccgcc
    21121 ttttgcaggt tgcggcgcat ccggccgtac tcataggcat agggcaggta cagggcctgc
    21181 tggtccaggc gcttgcccat atcctgagct gccggaccgg cattttccag gaagtaaatg
    21241 ttggccaccg attcacccat ggccttatat tcttcataag tctggcaggc agcgaaatcg
    21301 tggatagcat agcctccctg gcggagcata gccgtcaatt cattgtcttc gtcgacggga
    21361 tagtaattga cgacataatt gacggccttc tgtttcggca ggggctggac agcccgcacg
    21421 agctggcgcc ggccgaggat gtccggcgta tagcgccgct gcagggtcgg aatcatatag
    21481 ccgtcgatga aatcgatgtc gggatatttc tggcgcagcg tcccgtagac gacgcgcagg
    21541 tcgatattga ggaagtgctg catacagctg gtaaagcctt cgatgcactt cggccgctcg
    21601 ggcatatcgt cgatgatatg ggaaatgccg tcgatcatca tttcttccag gttgcccttt
    21661 atcaggttct cgttcgtcaa ggtgacggta gagaagcggc cggccccgtc cgggacttca
    21721 tcagccgaca agacgacgcc gtgaaggcag caggcgcagc aaacgtagac ttcgtggcag
    21781 ccagggatga gcatgggcga atgggcaatg gtccaatggc cccgggctgg cgaagcatat
    21841 ttcaggccga tgtggaaagg cttgggaaac gacgcctggc tgatggcgac ttcggcgccg
    21901 tctggtgtga cgtgataccg cggcacctgg ggtgaatgaa tttcattttc gtaaatcatg
    21961 ctctccctcc tccttagcag atgcgcggca gcagttcacc gcctggcggc ggcagcagcg
    22021 tttccgcgcc gatttccgta cgcatgatga cccggccggg ctggtcggct gtaacttcgc
    22081 cgatgatggc cgcatctttc gaatatttgc cctggcgcag ggtggcgaca atatcgccgg
    22141 ctacggcttt gggcgcaaag atgaccagcc ggccttcgca ggccaggtaa agcggttcca
    22201 ggccgagcat gccgcagacg ccgcggacgg ccgggtcgac cgggatggcc tggctgtcca
    22261 agtggatgcc gacgtggctc tggccggcga tttcatagag gaccgtaccg acaccgccgc
    22321 gggtcgcatc acggatgacg tggatgtcct tggtcgtatc cagcatggct tcgacagtcc
    22381 cccataaagg cgcgcagtcg ctggtcacat cggcatcgat gccgaactgg ttgcgggcca
    22441 ggaggatcgt gcagccatgg cggccgacgt cgccgctgac gatgacagca tcgccgggac
    22501 gggccagggt ccccgacgta tggacgccgt ctacgatctg gccgataccc gtcgtcgtaa
    22561 tgaacaggtg gtcgacctgg cctttcccgg ctactttcgt atcgccggcg acgatgcgca
    22621 caccggcttc agccgccgtc ttggccatag ccgccgcata ttcttcgact ttagccaggg
    22681 ggaagccttc ttcgatgacg aaggcacacg acagatataa gggcttggca cccatgcagg
    22741 acaggtcgtt gacggtcccg cagatgctca gtttgccgat attgccgcca ggaaattccg
    22801 gcggcgagac gatgaagccg tctgtcgtaa aagccaggtt agtcccgccg atgggcagga
    22861 cagcggcgtc gtccccggtg aactggggat tggaaaaatg agctttaaag acacggtcga
    22921 tgagttcggc agtctgtttg ccgccggcgc cgtgggctaa ggtaatgaca tcactcatgt
    22981 aaattgcctc catataaata ataagccgag caggcccctt cgtgggaaac catgcaggcc
    23041 cctacgggat gttccggcgt gcagaccttg ccaaagagcg ggcactgggg cggcgtgcat
    23101 ttcccctgca ggatatcgcc gcaacggcag gccggattgg cgcgcccttc catagccggc
    23161 acgccgtatt tcttgcgggc gtcacagact gcaaaggcag gccgcagctg caggcccgac
    23221 tgggggatga cgcctaagcc gcgccattcc gaatcgcacg gttccatgac ttcttttatc
    23281 agggcctgag cggccgggct gccttcagcc ttgacgacac ggggatagca gttcttgaag
    23341 aagggcttcc cctgagggaa tttgaccagg atgacggcaa aggccgtgag cagttctttg
    23401 gccgtaaagc cggcgacgac gccggatacg ccttctttca gcatggcttc gccagagcgg
    23461 gtccccatga cggcgtcgac gtggccggga tagaggaaag cgtcggcgct gtccttcatg
    23521 gcttcatagg ccatgggcat ggtcttgtta gccgtgagca gggaaaaatt cgtcatcccg
    23581 tcggcgacgg cctgcttgac agccaggcac gacggcggtg tcgtcgtttc aaagccgaca
    23641 gacaggaaga cgacgtcttc gtccggatgc gccttggcat aatcataggc gtcgaggggc
    23701 gaatagacga tttccacatg acctccctga ctgcgggcat cggccaggct catcgtactg
    23761 cccggtacgc gcacgaggtc gccaaaggtg cagatcgtaa cgcctttttc cagggccagc
    23821 cagatggcct ggtcgatata accgacaggc gtgacgcaga ccgggcagcc cggcccggag
    23881 atgacgtcga cctggggcgg caggatctgg cggatgccct ggcggaagat ttcatgcgta
    23941 tgggtcccgc atacttccat aatgcggatt ttcggcccgt cataactgcg gataatatcc
    24001 agggccgtcc gcttctttgc ttctgccatc agttcaggtc ctccaggata tcgtctgttt
    24061 cgtcggcatc attatcggta atcttggcga tagccatgcc ggcgtggacc atgacgtaat
    24121 cgccaggatc gagttcgtcg ataagagctg ccgatacttc ccgtttagca ccggaagcat
    24181 cgacgagagc cattccattc tgtattttca taacacgtgc cggtaaccct acacacatga
    24241 taattcctcc tttatgtata atcaattaat ttcgttcatc gaacaacggg caacctctca
    24301 gaccgccttc ggcggccagc tcccctatca ggggagcctt gcgacctgcg acctgcgacc
    24361 tgcggcctgc gggccgcctt ccaggcggcc cctacgcata cgcggcgtaa acggcctgtc
    24421 ctaaggcgat gccgccgtcg ttgggcggga ccgtgctgtg cttgaggacg gtgaagccca
    24481 ggtgctgcag ggtctggtcg caaaggcgca ggagcaggcg gttctggaag acgccgccgc
    24541 tcagggccac ggcgtgcagt cccgtttctt cctgcacggc caggcaggcc ccggcaatca
    24601 tggcggccag gccccgatga aatgcatagg ccaggcagcc ggccgcttct ccggccaggc
    24661 ggccttccag tatctgccgg accaggacac ccgtcggcag gagcagccgc tcttggccat
    24721 ggatgggcgc catgggcgtc acatccggtg cgggacggtc tttttcccag gcctcagcgg
    24781 caaactggag ggccatcgac gcctggcctt caaaggtcga agcccggcaa atccccaaga
    24841 tagcgctgat gccgtcgaag aggcggccgg cactggtcga caagacgctg ttgatctgct
    24901 ggtcagccag gaagaactgg gcctgccgtt cggcttcact gcaaaggtgc agggcttcga
    24961 tcatggacgc cgcctggctc cggtccttct gcaagctgtc gatgaggctg acggcaatgc
    25021 gccagccctc gcgggccgat gcgtcgccgc cgcgctgcca gaaaggctgg attgacgcag
    25081 accgctgaaa gccatggacg tcactgacca ggacttcgcc gccccaaatc gtcccgtctg
    25141 tcccgtagcc ggtaccgtca aaggcgatgc cgatgaccgg ttcttcccag tcgttttcag
    25201 ccatgcagga caggatatgg gcatagtgat gctggacggc gacgacgggc aggcccagtt
    25261 catgggctac ggccgtcgtg ttatagcgcg ggtgcaggtc gcaggcgacg acagacggct
    25321 tggcttcgag gagcgtcgtc atgcgccgga tggactggcg cagggcttcg acagtccgta
    25381 tatcggccat gtcgccgatg tacggcgaag gatagaacag gtgattgcgg ccgatgcaga
    25441 aacagttctt caattctcca cccatagcca ggacatcctt gccgcttccc tttccataga
    25501 agaaaggcag cggcgcatag ccccgggagc ggcgtatcat atagggcttg ccgtcatacc
    25561 agtccatgac cgaatcatcg gcccgcaggc ggatgcggcg gttgtggctc aggatgaggt
    25621 cgcagaggcc ggacagttcc tgccgcgctt cgtcgtcatc gtgacagata ggcgcgccgg
    25681 actcattggc gctggtcatg accagtgcat ccggtacggc gaggccgtcg tcataagtaa
    25741 agagcagcag atggagcggc gtatagggca gcatgacgcc gacagacgga ttgcccggtg
    25801 caatggacgg cgccagggtc tgactgcctt tcttgggcag taagataatc ggcttctgat
    25861 ggccgtcgag gacgttttcc gccccatcgg gaatgatgca ctgccggcgc acggcgtcga
    25921 ggtccttcat catgacggca aaaggcttta caggccgccg cttgcgctga cgcagggtct
    25981 ggacagcctg ttcattgcgg gcatcacagc agaggtggaa gccgccgatg cccttgacgg
    26041 cgatgatttt tccatcaata atcgcctgtc tggccgcaat gatggcctga cggcgccgtt
    26101 cgggccggtc caggagatag acttccggac cgcagtcgtt gcagcagacc ggctgggcat
    26161 cgtaacgccg cgtttcggcg tgggtatatt catactcaca ctggggacac atggggaact
    26221 cgcccatact cgtccgttcg cggtcgtaag gcatggcatc gaggatggtc agccgcgggc
    26281 cacaggccgt gcagttgatg aaggggtgga gatagcggcg gttggtctta tcgaagagct
    26341 ccgctttgca ggcatcgcag atggcaatgt ccggcgacac gaagatatcg cctttttccc
    26401 tggcgctctc gacgatggaa aaatcctcgt acggcggccg gtcttccgtc gtcgtgacga
    26461 ctttcaggat agccgaccgt tcaggcgcct tgccttccaa atcatcgtgg aaggccatca
    26521 gcgccgccgg ggaaccttgg gccaggattt ccacataaga gcccttattg cagaccgtcc
    26581 cgtgaatggc atgagccgct gcgatgcggc tgacaaaggg ccgaaagccg acgccctgga
    26641 cgatgccaaa gacacaaatc cgttccgtat gcatcttatc ccctcccgga cacggcgaaa
    26701 tgcgggaaat cgacgtattg gccgtggaag ttcggcaggg cccggcggaa ataggcatcg
    26761 ccgaggatga tgtcgaactg gtcgttgtcg gccaggcggc gcaggtcgtc ttcttcacgg
    26821 agccggacac agtccgtccc ctgcggctca gggagcccca tgaaccacga agccgtcgtc
    26881 acgtaagcgg caccggcttc ttcggccatg gcggccatag ccgaggcagc gacagcctga
    26941 tggatgatga ggacgttctt gccagaaaag aggttgtcgt caaaatcgac gacggcatcg
    27001 aggccgaagt aatggagctc ataaggtgtc ccgaaggtct tctgcagata ttgagcggcc
    27061 ttcaggccgg ccggtgagag gaccagattg agggaggcct tgccggcctt gcggtaatcg
    27121 tcgatttcat cgaacaagag aatctgctgc cagccgtctt cgaccaggga ctggctgatc
    27181 cattcgatat cgccagggtg catcagttcc aggggaacag cgccgaggac gccgactgta
    27241 tcggcttcgg cctgctgcga cgggtcggcc aggaccggga ataaggcgtc gtaggccttt
    27301 tcgatgccct tgtcgtagta atccatgccc gtcgtatcga ctgtgacgac tgggatggac
    27361 agcttctttt cagccatgtg cttcaaggcc ttatagtccg tgccgatgac cgacgggacc
    27421 ggcgtcccga cgatagcggc aaagtcggca tcaatcgttt cggcagcctg gcagagttta
    27481 tcgaccaggc ggtcgtcgcg gccgaggatg gcgtccatat cgcgcaggcc ggcactgaag
    27541 acggcactct ttttcccact gaaccagcgc ggttcatcga aaccgcagat gttgccggca
    27601 cagccgccgg catcgcaaat gacgacgatg ccgttcagtc cgtagaggac ggccgccgcg
    27661 ccggagtcgt ccggtgcaaa gggtgaaata tatttccata aacctttcat ggatgtttcc
    27721 tttccaacaa ggcctgctcc agccgctgta acagtgaaat gactccggca taaccgaagg
    27781 gctggatatc ttcatcccac gagagatggg cggcctgttc atgataatat gcggcatctt
    27841 tgccgatggt gacgtcgaca ggatggtcct gtccccggta atagagcatg gacggcgaca
    27901 aattggagta tacctgcgta tccgggctca gctgggccag gtggcgcagg tagacgaaat
    27961 cgtccggccc caggttggcg tagatttcgc ggatgacgaa accgtagcgg gtcagggcca
    28021 gggccatttc aaaggcgttg ccgttctgca tctcgccgat ggccacggtc gtgccgctgc
    28081 atttttcccg cacagcccga acgacggcct gggcccgttc atgccaggca ctgtcatcga
    28141 tggtcgtccc cagtacctga gccagggcct gatactgttt gtggatctgg gcgacctggt
    28201 agagacgggt cagctcgatg ctgggaatac ccaggcgctt ttccatatcc tgggcagcca
    28261 ggcgcgcttc cggattgagg atgatgttga cgttggcctg ggacatggcc ttataggcgg
    28321 caaagtcggg acagcgggaa atctcattga tctgattaaa gccgacgcct ctcaggatgt
    28381 cgtacaactc gcagtcgtcc tgcaagggcg cgaaatagcc caggagattg accgtccggc
    28441 ggcggcgcgg ctgtttttcc agcagcgaat aaatggcccg gcggacgtcg accatcggcg
    28501 gcttgcggcc ttcccgggtc agagcgtaca tgtagcaggg cacgacgggc aggcccgctt
    28561 ccttttcggc tttgcggcag acccgttcca tatcggtccc cagaagggcg tcgacacagg
    28621 tcatgcagat catgacgact gacggctttt cagccaggct gtcgacgact tcacagacgg
    28681 cctgggggat tttcttcaga tggcggcccg tgacgatgtc cgtttcatcc atgatataga
    28741 agaagaaacg gtcgctgtac ccgcccattt cgctgaggat cgtcgtattg cggccacagc
    28801 agcccggtcc gaccaggagc atgaccgatc cgggaatgga caggcctgcc cgtttgacgc
    28861 cgaagccctg ggccccaggc gagttgaagg ccagggcagc cggagaatta tagatgaggt
    28921 gcgtcgacgt gacgagttca gccgggatat catcgcggcc gcgcgtcacc aggtcctgca
    28981 cggtgatagc ataaggtttt cgttctgtca taatcattcc ttgtcttgta ataatttttg
    29041 ggccaaatcg aggaagatat ggctgatagg caggtccgga tcgccttcga tgacggtctg
    29101 gcccagatct tcatagcggt tgatgtcgtc gctgcgggga atgtcgccga cgatggaaag
    29161 gtgagccttt tcggcaaagg cccggacctt cgcttcttcg tcggggacgt tgcggcggtt
    29221 gaggaggata ccccggacct tggcatagct gcggtcggca aaattggcca cagccgtatt
    29281 gatgttgttg gctgcgtaga gagccatttt ttcgcccgat gtgacgatga ggacctgttc
    29341 cgcatagcct tcgcggatag gcgctgcaaa gccaccgcag acgacgtcgc ccaggacatc
    29401 gtacaggacg acgtcgggct catatttttc aaagaggcct aaatcttcca acaaagaaaa
    29461 agtggcgata atgccccggc cggcacagcc caggcccggc gtaggaccgc ccgtttcgat
    29521 acagaggaca ccgccatagc cgatgcggga aatatcctcg atggattctg gatcttcgtc
    29581 gtgttcccgc agataattca tgaccggctg gacgggatgg ccgccgagca gattgaacgt
    29641 cgaatcggcc ttagggtcac agccgatctg gatgactttc ttgcctaaat aggcgaaggc
    29701 agcggccaga ttactggtca tcgtcgattt cccgatgccg cctttgccgt aaatcgcaat
    29761 tttcagcata cgcgcctcct actgcggcat aggcgggtaa atcgtcttgg tgctgacttc
    29821 cgggagcatc cctaatttcc ccgacaaagc gctgatgacg tcttgcggcg catcgacgat
    29881 gaggctgatg acgttgatat ccttatcctt ataagggacc cccatgcggc cgacgacata
    29941 ctggccatat tcatgcagga tctcatttaa tttagtcgat tcctggcgat tccctacgat
    30001 aataccgatt aaagcgactc tcgtttccat attcgtacct ccctaaagaa aaaaggggct
    30061 gtcgcacgtg cgacgcccct ttttgagttt taagtttgca gtttgtagtt ttt
//
LOCUS       DAWABZ010000006        29911 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_444_length_29911_cov_3.650723, whole genome shotgun sequence.
ACCESSION   DAWABZ010000006 DAWABZ010000000
VERSION     DAWABZ010000006.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 29911)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 29911)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..29911
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_444_length_29911_cov_3.650723"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    10496..10505
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17510..17519
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    27311..27320
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6259 a         8203 c         8919 g         6500 t
ORIGIN      
        1 ctcgcacgtt ggcgttcatt cagttcattg ttcagttttc aaaggtcatc gttgccatcg
       61 cttcatcgcg acgacttgta tatcttacca aactccgaga agtctgtcaa gccctgattt
      121 gaactttttt caactcaatc agtgccggtc cgctaagcga actcatacag acacaacaaa
      181 acaaacctat cgtttctttc gtccgtctca gtacagctgg ttcctatata atagcattta
      241 gcttgcaaaa tgtcaacact atttttctaa cagggaaaaa taacggtgga tatggcaggc
      301 caggaagacc gtgaaccgct gttcccagtc agccagatca tagggaatga gtttctccgc
      361 cttgtggatg cgataggcta cggtattgcg atgggtgtag ggctgggccg ctgttccctt
      421 caggctgcca tcgtggcaga gataggcttc cagcgtttcg tacaaactgg accggtggct
      481 ttcatcataa gccagcaagg ctcccagggc ccggtcgtgt aggacgcgca gcacccggtc
      541 atcgggctga gaaaagaaga gggcataggc gccgagggct ccgaaatagc accaggactg
      601 ctgatggaga cgggcccata ccaaagcctg ctgcccctgg cggcagctgt ctggcagggt
      661 tttcaagtca ggcgccgtca ggccctggcc cgtcaccagg cggtcatggc ctttgagcca
      721 ggcgacgagt gctgccggca gcgttccctg ctggagcagg acgatcgtgt ccttataagg
      781 aaatataaag tatggctggt ccagcccgtt gaggaaggat ttgctgtcag ccaaaaaatc
      841 ggcatcgcca tcggcgccgg ccaggaccag tgccgtatac ggcccctctt cctgccagcc
      901 cgtcagagcc agttcagtca ggaccgacga cccctggacg ccgaagagga attccttcca
      961 cgccgtaatg gcccggtatt cttcacggtt ggtcaaaaag agggccgaca aaaaggactg
     1021 ggtaatgtcg gccaggcgca cttcccaagg catggtgaat atcgggaagg ccgcttcctc
     1081 acagcgttct ttcaaggctt ccgtaatatc cgacggcaag aggtactttc ccgtattgac
     1141 gaccaggccg gccgcatcgg ctgccagcag ggcatcgacg aagttttcca gccaatgcgg
     1201 gtcgccgggg ctggccaggc ccgtcgtgaa gatgaaggca tgaggccgca aaaaagtgcc
     1261 atagtctgca tcttccatga cctgcaccca ggccacgtcg tggctcagtc cctgccgtcc
     1321 ggccaggagg tccagtttat atttgttttc atcctgcctg taaagttgag cgatggatag
     1381 agacatggga ctccctcctt tgtgtcgtca gcacaacatc atatttttaa ctttggtttc
     1441 ccatcttctt gttgttttta atagtaaaac gtataataag cgtatcattt aagattatcc
     1501 atgcaaagtc tttcatagag cactcaccaa ggctttgtat cttgcattga taagggggag
     1561 acattatgtt aaaagatgaa ttaccgaaaa ttgttaccca gaccgttccc ggtccgaaag
     1621 ccgctgccgt cctggcccgc cgccgtcagg ccatcccgga tgccatcggc accatctatc
     1681 cgacagtcat ccagcgcggc gaaggcgcca tgttcgaaga catagacggc aactatttcc
     1741 tcgactgggt cggcggcgtc ggcgtcctga acatcggcta ttcccagccg gaagtcgtca
     1801 aagccgtcca ggaccaggcc gggaagtatt tccacgccat ggccaacatc gtcacccatg
     1861 aaggctacgt cgccctggcg gaaaaactca acgccatcgc gccggtcaag ggcgacaaga
     1921 agcgctcctt cttcatcaac agcggcgctg aagccgatga aaatgccgtc aaagtcgcca
     1981 aggcctatac gaaacggccg aacatcatcg tcttctccgg cgccttccac ggccgcacgc
     2041 tcatgaccat ggccatgacg gccaagaaag cctacgcccg cggcatgggc cccttcccgg
     2101 acggcgtcta tcgggccgaa ttcccttatc tctaccgcaa accggctggc atgagcgatg
     2161 acgaagccct ggaattctac ctgaattccc tgaaagaagt cttcatgtac gcttcgccgg
     2221 cggaatacgt cgccgccatc gtcgtcgaac cgctccaggg cgaaggcggc ttcatcccgg
     2281 ctcctatccc ctggatcaag gccctgcgca aagtctgcga cgacaacggc atcctgctca
     2341 tcgccgacga agtccagacc ggctggtgcc gcacaggccg catgctggcg acggaatact
     2401 ggaaagaagc cggctgcccg ccggatatca tcgccacggc taaatccatc ggcggcggcg
     2461 tgcccctcgg tgccgtcgta gcccgcgaag aaatcatgga atccgtcccg gccggcgtca
     2521 tcggcggtac ctttggcggc aacgccctgg cctgcgcttc ggccctgaaa gtcatcgaaa
     2581 tcatggaacg ggaccatttg gccgaccggg ccctggaaat cggccagaaa gtctgcgccc
     2641 gctatgaaca gtggaaacag aaatacgacg tcatcggcga ctaccgcggc ttaggcgcta
     2701 tgctcggcct ggaattcgtc cacgacaaag ccagcaagaa gccctatccg gaactggtca
     2761 cagccgtcat caaagaagcg gcccaacacg gcctgatcat ggaaaactgc ggcatttacg
     2821 acaacgtcat ccgcttcctc tgcccgctcg tcgtcaccga cgcccagctc gatgccggcc
     2881 tggacatcct ggaacaggcc atcattacct gcacgaaata atcagtattt ctctcccatg
     2941 acatcgaggg cgaccatggc catggccttg gcgcccgtca atgcctgcat cttgcccagg
     3001 tcgctgccag cggcttgggc aaattctttc gtatgggcat tgatgtcgta gccgatgcag
     3061 atttccggct ggctgacccg cgtaatttgg ctgacattgc ctacgtccgt cgagccttcg
     3121 gcatagtcgt cgtcgcggat gccgaattct tcgtagagct ggttgaaggc gtcggcaaag
     3181 taaacgtcca tcctggggtc attgatcata ttcttataga gcggttcata atggaccatt
     3241 tccactttcg ctccgtgtgc gtcggcgacg tcacgggcga ttttttttat gtccggcagc
     3301 atcttgtctt ccaggtattc cactttcagg gcccggatag tcgccttgag gacagcccgg
     3361 tcaggcacga tgttcggcgc cgtaccgcct tcggtgatga tggcgccgat gatatggcgt
     3421 tcggtcaagg tcttttcata ggccttcagg cagccataga aatcgacgag ggcatcgagg
     3481 gcattgacgc cgtggtaggt cgcatcggcg acgtgggccg ccttgccgag gaaggcgaac
     3541 tgcatggggt gcgtcgcata ggacgtgccg ccgatatagg tcttatccgt accgggatgg
     3601 acgatgacca ccgcgtccag gccgtcgaag acgccgtgtt cgctcatata gactttgctg
     3661 cccaccgttt cttcggccgg acagccgtag acgtggaccg tcgccttgtc gccggccgct
     3721 ttggcaaagg ccagggccgc gccgacgctc atggcggcga tgaggttatg gccgcagccg
     3781 tggccgattt ccggcagggc atcgtattcg gccaaaaagc ccatctgaaa aggaccgtgg
     3841 ccgcagacag cgtgaaaggc cgtcgggaaa gcgtcggcga cgggattgtc gatggcaaag
     3901 ccgcggtcct gcaggatttt tttcagcgtc gccgatgcga agacttcgtt catgcccgtt
     3961 tccgggtggt cgtgaaggct ttcggcaatg ccgtagatga ccggtttcag ttcatctaca
     4021 tagcggcagg cctggtcttt caaatcttta tattccatga gaatctctcc tatccaaatt
     4081 acagcagcgt aaagcgtaca ccgtccaggc gggcttcgag accggccatc tggcccaggg
     4141 gcagccaggc gttgtcggca ccgtggccga agggcaggcc catgacggcc ggtttgcccc
     4201 acttgcgggc ataagcctgg aggacttctt tcaccgtcca ttcataacgg gcgccttcgt
     4261 cagggccgca gtggtggaat tcgccgaagg cgatggcccg gacgcggtcg atgaggcccg
     4321 attcctcgaa ctggcagagc atgcggtcca gggcataggc ctcttccccg acttcttcca
     4381 ggcagaggat ggcgccgtct ggcggatcga tggcataagg cgtgcccatg aggaccgaca
     4441 ggagcatcat attgcccccg gccagcgggc ccgtgacggc accggggacg agggtttccg
     4501 gctggcagcc cggcggcagc ggcagttccc gcgcttcata gggatgctgc aggccgtagg
     4561 cgaactgctc tttcgtatat tccgaggcat tgcggcccag ggacatgacc atagtcccgt
     4621 ggatgggaca gaagccgcag cgctggagga aggccgtatg cagggccgta atatcgctga
     4681 agccgacgaa caatttggga tgggccgcaa tgagctcata gtccaagagc ggcaggatgc
     4741 gcgtcgcccc atagccgcca cgcaggcaga cgatggcctt gacggcgtcg tcggcaaagg
     4801 cttcgttgat atcccttgcc cgcagccggt cggggccggc cagatagcca tcaaaggcag
     4861 atacggatct cccgattttc acttcatagc cgagggaccg caagaaggtg aggcccttga
     4921 tcacgtcgtc accggccagg gctgacgacg gggcgacgac ggcgatggtg tcgccagctt
     4981 gcaacggttc tgcataggta tactccataa ggctacttcc tttctgattc atcgatatag
     5041 gctttcttga aatcgaccag tcccagcggc gaccagtggt agcccttgat atagggctgg
     5101 gccacgtagg gctgggtcgt gtagtacaag gggatgatga cgcagtcgtc gaagagtatc
     5161 tgttccgctt cgtggagata ggccatgcgc tgtctttcgt cgttgctggc cttggctttg
     5221 gcaatcaatg cattatacgc cgggttgtgg tactgggcgt cattttcgtc atcggcaaag
     5281 acgtcgagga aggtcatggg gtcgatgtaa tcggcgatcc acgacgcccg ggccagctgg
     5341 tagtcgcctt gggcgcgggt ggccatgaag actttcgatt cctggttggt aatattgacg
     5401 tcgacgccga ggttttcttt ccacatggcc tgcagggctt cggctacagc cttgtgcatt
     5461 tcattggtat tgaagaggag cgtcacttcc ggcagcgggt gctggtcatc gtaaccggct
     5521 tccttcagga gctggcgggc tttggccggg tcttcggcga tgaggttacc cccttcctta
     5581 cggaaatcac ggcctgtggc cttgtccttc agtcccggcg ggacccaggc gtaggccgct
     5641 tccttttcac cgcggacgat gtacttcatc agtccctggc gctggacggc cagggcaaag
     5701 gccttgcgga cgcggacgtc gtcaaagggc ttcttctgga cgttgaagac gtagtaatag
     5761 gcgccgagat aaggagcaat ctcgtagagg cccatctgtt ccagccgttc ctggtcggcc
     5821 ggcggcggtt cgacggtcat gttggcctga ttgctttcga ccagggtcag gcgcgtcgcc
     5881 tgggaatcgc tgatggggaa atcgaggtgg tccaggatga ccgaaccggc atcccagtag
     5941 cggtcgttct tgacgacttc gatttcactg gaatggttcc aggaaacgat tttatagggg
     6001 ccgctgcaga ccatgccgtc cgcatcggca gcccacgtat cgggcttggc ttcgacgagc
     6061 ttcctgggga cgggataata gcagtggaac gccgtcaggt tgaggaaata cggtgtcggt
     6121 tccttcaggc ggacgaccag ggtccggtca tcgacggctt tgacgccgac ggcatcggcc
     6181 gaggccttct tattgaaata gtcttcggcc ttgtcgatgc aaaagagcat ataggcattt
     6241 tccgaagcta cgtcgggatc gacgacgcgc ttccaggaat attcgatgtc gtctgccgta
     6301 atcggcgtgc cgtcgctcca ttcgatgccc ggccgcaggt ggaaggtata cgtcttgcca
     6361 tccggtgaga tatcccagct ttcggccagg gccggctggg gtacatcgtc atcgtcgagg
     6421 cgggtcaggc cttcaaatat ctgcagttcc acattgcttt cgacgagggc cgtcgtcatg
     6481 gccgggtcca gccgcgacgg ttccgcttcc aggacgtagc tgaccgtatc ggggtgctgg
     6541 atgccgcagc cgctgaagag gacggccgtc agcaggcagc tcaggagtaa gagtaatttc
     6601 ttcatcgtct gcctcctctc gcatgatggc aggcgacgaa gcggtcgtct gcgacctgcc
     6661 ggaaatgagg cacgtcgatg cggcagactt catcggcgta gggacagcgg ggatggaaga
     6721 cgcagccctt gggcaagtcg agggggctcg gcacttcgcc gcagacgatg gtccggcgcc
     6781 ggtagtgcgg gtccggccgg ggcacggagg cgatgagcat ctgcgtatag gggtggagcg
     6841 ggttgtggaa gacggcgtcg cccggtccca gttcgacgag gcggcctaaa tacatgacgc
     6901 ccatgcggcg gctcatgtga tggaccatgg ccaggtcatg ggcgatgaac agataggaca
     6961 ggccccgttc ctgctgcagg cgctgcatca tgttgacaat ctggacctgg acggacacgt
     7021 cgagggccga aataggttca tcgcagaaga cacattccgg gtcgacggcc agggcccggg
     7081 cgatgccgat gcgctggcgc tggccgccgc tgaattcatg gggatagcgc tgggccgctt
     7141 caggccgcat gtcgacctgt tccaataagg acgccacgcg ctggcgccgc tggctgtcgt
     7201 cctgacagag atggtagttg cgtaagggct cgctgatgat gtcttcgacg gtcatgcgcg
     7261 ggtccagcga ggcataaggg tcctggaaaa tcatctggac gtggcggcga tagtcgcggc
     7321 ctacagcgcg gtcggcaatg tccctgccct tataggtaat ggacccgccg gtcttggcat
     7381 ataagcccag cagggtccgg gcaaaagtcg atttgccgca gcccgattcg ccgacgaggc
     7441 cgaccgtctc gccctggccg atggtcaggg acaagtcgtc gacggcggtc agggtcttct
     7501 tgggccgtcc cagccagtcc cgctggacga cgaattgttt actgatatgt ttggcttcga
     7561 tgaggttcat ggcaatgcct ccttggccgc ggcggcctgg gccagccagc acgtgtaatg
     7621 atggcccggt ccggcttctt cccgcggcgg cgccgctgtc atgcaggcct tcatggcctg
     7681 ggggcagcgc ggatagaaac ggcagccctc gggcatgtgg aagaggtccg gcgcctggcc
     7741 ggggatcccg gtcagggctt tatccttctg ggccgggtcc ggcagtgagg ccaggaggcc
     7801 ctgggtatag ggatgggccg ggcggtagaa aatatcatcg gccgtccccg cttcgacgat
     7861 ggcgccggca tacatgacgt aaatgcggcg gcacatctgg gcgatgacgc cgagattatg
     7921 ggaaatgagg acgacggcca tgcccaattc cgcctggagg cgcttgagga catccaggac
     7981 ctgggcttct gtcgtcacgt cgagggccgt cgtcggttcg tcggcaaaga ggagccgcgg
     8041 cgaacaggac agggccatgg cgatgagaca gcgctggcgc atgccgcccg ataattcatg
     8101 gggatattga tggagccgct tttccggcga cgtcagtccg acggctctga gcagttccag
     8161 ggccttggct tcacaggctt tacggcccag gtgctggtgc agggcgatgc cctcgcagag
     8221 ctggttgccg atggtcagga cgggattgag cgacgtcatg gggtcctgga aaatcatggc
     8281 catgtcgcgg ccgcgcaggc ggttcatctg cgtttccgac aaggggatga catcgcggcc
     8341 gtcccagagg atctggccgc cagtatattc cgtcgcttcg gccggcagca gcttcatggc
     8401 cgcctgggac gtgacggatt taccgcagcc cgattcgccg acgatgccga cggtttcgcc
     8461 gggattgacg gtcagcgata cgtcgtggac ggcttccagc agtccccggt aagtatggaa
     8521 agaaatggtc agatggcgta catctaataa ggcttgcatc agcgtccctc cttctggttc
     8581 tgcgggtcca gggcgtcgcg caggccgtcg ccgaggaaca tgaagcccag catggtgatg
     8641 cacagggcca gggccgggaa gacgagctgg aacgggaagg agcgcatact attgatacct
     8701 tctgaagcca ggacacccca gctggccatg ggcgccgata cgccgaggcc gatgaaactc
     8761 aggaaggctt ctgtaaaaat cgcgtcggga atggacaagg tcagggtgac gataatcggc
     8821 ccgacgcagt tgggaatcat atgacggcag aggatatgcc acgtcgacgt cccgcaggag
     8881 cgggcggcca tgacgtattc ctgctgcttc aggctgagga tctggccgcg gacgatgcgg
     8941 gccatgttga gccagtaggc aatgcccagg gccaggtaga tgttgagcag gcccggcttg
     9001 aagacgacca tgagcaaaat gacgtagagc agcatgggaa tggaatagag gatgtcgacg
     9061 atatgcatca tgatctggtc cgtccggccg ccgacgaggc cggcaatgcc gccgtagatg
     9121 acgccgatga agacgttgat caggctggcg acgaggccga tggacaggga aatgcgggcg
     9181 ccgtagagga cgcggacgaa gaggtcacgg cccagggaat ccgtaccgaa ccagtgcgca
     9241 gccgacggcc aggcattggc tgccgtcaaa tcctggtcgg cataagtata gggcgccagc
     9301 caggggccgg caatggccag gaggctcatg acgacgacga tggccaggcc ccataaggcc
     9361 aggcggttct tcttcatctg ccgccagccc cggtcgcgca gggtctgggg cggtacatag
     9421 ggttccgtcc ggtcagcagc cgtaagcggt gtaaaatcca tcatcagccc tccttctttc
     9481 catccatggt gatccgcggg tccaagagcg gataaatgac atcgatgagt aaattcatca
     9541 tcatgatgag gaaactgtaa aaaatcgtaa tgcccaagat gaccgtatag tcgcggttat
     9601 aaatgctggt gacgaagtgg cgtcccaggc cggggatggc aaaaatcgtc tcgacgatga
     9661 agctccccgt catgagggcc gccgccaaag ggccgatata actgacgaca ggcagcaggg
     9721 cgttgcgcag ggcatggtgg cagatgaggg accgcgtgcc gatgccccgc gaccggaccg
     9781 tgcggatata gtcctgcccc agggcatcga gcaggctcga ccgggtcagg cgcatgatga
     9841 aggccgtcgg ctgggccgcc agggccaggg ccggcaggac aacgtaagcc ggccccttcc
     9901 acagggccgc cggcaggatg ggccaggtaa aggcgaagag ctggatgagg accgctgcca
     9961 ggatgaaact gggcacggag acgcccaggg tggcgccgac catcatggtg tagtcgatcc
    10021 aggtattctt ataccaggcc gcggccatgc cggccaggac gccggcaccg atggccaaaa
    10081 agaggctgat gagccccagc tgggccgata cgggaaaggt ttcggcaatg atatcgttga
    10141 cggtcttgcc gggatactta taagaaggcc ccaagtcgag gaccgctgca tggcggacgt
    10201 aatcgacgta ctgggaccat aagggttcgt cgaggtgata tcgggcttcg acggttgcca
    10261 tgacggccgg cggcagtttc ttttcctgcg taaacgggcc gcctgggatg gcatgcatga
    10321 ggccaaaagt aatggtgatg atcgcccata agacgacaca agcgccggcc aggcgctttg
    10381 caaggtacat ggccaattca ggttccttcc tttccaacat agatattatt cattataagg
    10441 ctgtgttccc atcgtgacaa gttgtaatcc ccgaaaaggc ggcgtataat aagaannnnn
    10501 nnnnngtacg ttaacaatcc atacgagaaa ggtgatcgcc gtgaactgga aattctgtct
    10561 ggccattctg acctgcaacg tcgtcttcat gtcttcaagc tataccatgc tcatcccctt
    10621 cctgcccatg tacctgacgc gggaactggg cgtcgatgcg gcctcggtca acatctggtc
    10681 cggtgtcgtc ttttcgtcga ccttcctcat cagcgccatc atggcgccta tctggggacg
    10741 catggccgac cggcggggca agcggctcat ggccatccgc gccagcctgc tcttatcgat
    10801 cagctatttt ctcggcggca tcgtcacgtc gccgctgcag ctgacgttca tgcgcatgtt
    10861 ccagggcttc gcatcgggcc tgtggccgat ggaactggcc atcatgacca tctatgcacc
    10921 gccgaagaaa ctgggcatct gcctgggcat catgcagggc gccctgacgg caggcggcat
    10981 catcggcccc ctcttcggcg gcatcctggc cgaagccttc ggcatgcgca tgtccttctt
    11041 cctggcagcc ggcgccctgt tcctcaactt cgtcgtcctg gtcttcttca tcaaagaacc
    11101 gccgacggat acagccgcag cggatacggc cgcagccggg gcagaaggcg aaaaggtcag
    11161 cctctggaag attcccgtca tccgcctgat gctggtcagt gccgccctgg tccaggtcgt
    11221 catcctcatc gtccagccca tattgacgac atacatctcc cacctggccg gcgagctgga
    11281 caacctggtc ttcatttccg gcctcatctt ctccatgagc ggcttttcca gcgccattac
    11341 ggctccgctc tggggacgct tcggccagca tcacggcttc gtcaaggccc tgcgcctgtc
    11401 cctggtcctg gcggggattt tcttcttcat ccaggccatc ccggacacgc tgtatacctt
    11461 tgcggccagt cagttcgcca tcggcctctt cttctccggc atctatccgt cgatcaacgc
    11521 catcctggct gaaaagacca gtgccaatat caaaggccgt gtcttcggcc tgatgttctc
    11581 ggcccagcag gtcggggcca tgggcggccc tatcctgggc ggcgtcatcg ccaccttcct
    11641 gggcatgaaa tgcgtcttcc tggcagccgg cgccctgctg ctcgtcatca gtatcgccgt
    11701 ccagcgcaaa gcccgccagc tccacgaagc ctagccccgc gtgccgcggt tgtgggtgta
    11761 attgggcagg agcttcggcg acaggatatc gtcgtctacg ccatactgtt cccgttcttc
    11821 atcgaaacgg cggacgtggt ccagcgtccc tttcccagga tgggccgtct ggctgtagcc
    11881 agccgcttcc gtcccggcaa tgcggccgaa gacgatgata tccaggaggc tgttgcccat
    11941 gaggcggttc gtcccgtgga taccgccgac gtcttcaccg gcggcataga ggccgggtat
    12001 cttcgtttta ctcttagcgc cgatacagat gccgccattc tggtaatgca gggtcggata
    12061 gaccaggatg ggctgcttgc ggatattgat gccaaatttt tcatacatgt ggatcatggc
    12121 cggcaggcgc ttttccatgg tcccttcgcc gttgatgagg tcgaccatgg gcgtgtcgag
    12181 ccagacagcc ggtccgtgga gggcatagac ccctttgtcc cgttcctgac attcgcggat
    12241 gatggccgct gcgacgacgt cacgcgtttc caggggatgg aggaaggctt cgccgttctt
    12301 attgaccagc atggccccga gagagcggac cttttccgtc accagggcgc cgtagatctg
    12361 cggcggatag gcagcgcccg tcggatggta ctggatggcg tcggcataga tgaggggcac
    12421 gccggcgcgg taggccatga ccaggccgtc ggccgtcgag ccgtagtgat tggacgttgg
    12481 gaagccctgg tagtggaggc ggccggcacc gcctgtggcg atgatgacgc tcttggcccg
    12541 gacgacgtgg tattcccggg tatcgaagtc atagaggacg gcaccggccg ccttcccttc
    12601 gccatcgagg aggagttcca cagccggtga atattcgacg acgtcgacgc cggcattgcg
    12661 cacttcgtcg cggaggacgc gcatgatttc cgcccctgta tagtcgccgc aggcgtgcat
    12721 acgcttgcgc gacgtgccgc cgccatgggt cgtgaccatg gagccgtcgc tgttcttgtc
    12781 gaagaggacg cccagcgagc tgagccactg gatggcttcc ggcccgtgga ggacgagttc
    12841 ggccagcagt tcaggcttgt tcttataatg gccgccgccg atggcgtcga gatagtgggc
    12901 ctccggcgaa tcgctttcct tgtcggctgc ctggatgccg ccttcggcca tgcccgtatt
    12961 ggcatcgcct atacgcagtt tcgtcgccat gaggaccttg gcgccgtgcc gtgccgcttc
    13021 gatggcagcc gccgcaccgg cgccgccacc gccgatgacc aggacatccg tttcgaactg
    13081 gatgtgatcc aaatcgacgt cagccgtgtc catgcggctc ttgccctgca gcagggcagc
    13141 cagttccttc ggcactttat cgcctttatt ggggccgacg gtcaggacgg taaagccttc
    13201 ttcatggtaa tccggatggt actggcgcag gagttccccc ttctcttcag ccgtgaagcg
    13261 cggcagttct tcagacaaac ggatattccg cgtagcttcg acccgcgcct gggaagcccg
    13321 catttcttct gtataaatca tgatcgtccc cctatttctc aatatcccgc gattcatata
    13381 agcggcgtaa ttcatcgtca ccggctgcca tcagatgctg gacgtcggcc acgaactggc
    13441 cttcccggat ggcctggacg cgctggtcca ggtgcctgct cttcttggcg atgtacttgc
    13501 ccatgaggcg ccgtgccagg aggccgacgg caccgtgact gatcttggcc gggcagctgg
    13561 cagcgcagat gttgcagcag acacagcgga aggattcctt ggcacagccg gcataatcac
    13621 cgcgctgggc cagggcgatg tacttcatga cctggatgcc ctggggacag ttccgcgtac
    13681 aggcgttgca gtggatgcag ttgtagattt ccggatagag ctggccgacg acgttgccgg
    13741 ctacgggcag gtcgtccata tcgtaagtct gtttcttgat gggaaatgat tccaggcggc
    13801 cgacgcacat gccgtcttcg actttcgtct ggcaggccag ggccgaatgg gtctccgtct
    13861 ggccctgcag gcggtagacg acggcacagg ccccgcagaa cccgctgcgg cagccgcagc
    13921 cgcggaccaa gcggaaaccg gaatattcca tggcccgcat gatcgtcaaa tcttcaggga
    13981 ccgaaaattt cttgcccagt aaatagaccg ttaccatttt cataatgata aaaactcctt
    14041 tgtgaatgaa tggcaaaaga ggctgtcgta ctaagacagc ctctttttag ttgatagttt
    14101 gtagttttta gtttgtagtg aatggctaaa aatttaaccc tcaactacta caaactacaa
    14161 actttatact acataacgat tattccagta cttccaacag gcagtcaccg ctgatgatgg
    14221 cctggtcttt ggtggcgtag atttcgccga ctgtaccgtc gataggcgac gtgatggtcg
    14281 tttccatctt catggcttcg gtaacgacca agggttcgcc tttcttgact ttctggcctt
    14341 tcgtgaccag gactttgacg acggagccgg acagggttgc accgatgtcg ccggctttgt
    14401 cgggatcggc cttgcggctg gtgacggact tcatatcgac gctcttatcc tggacctgga
    14461 tttcgcgttc cgcaccgttg aacatgaagg taatcgtgcg gacaccactg tcatcgggat
    14521 cactgatgtt gatgagagtg atgatgaggt ccttcccttc ttcgatttca acgtggactt
    14581 cttcgttctt cttcatgccg aagaagaagg tcggtgtatc gaggacgctg acatcgccgt
    14641 atttcttgac catttcgttg aagtccttga agactttcgg atactggcag taagcgttga
    14701 tgtcttcgtc ttcgtgtttg taaccggcat cggtcaattt cttgcggatc gcttcgaaat
    14761 cgacaggagc caggctcttg cccgggcgtt ccgtaagggg tttcttgcct ttcaggacga
    14821 tcttctggag tttttccggg aagccctgat aagggatacc gatgcggcct tcaaagaatt
    14881 cgacgacaga ctgcgggaag tcgaggacgt cgcctttatc gtagacatcc tgttccgtca
    14941 ggttgttctg gaccatgaac aaggtcatgt cgccgactac tttcgacgac ggcgtaactt
    15001 tgatgatgtc gccgaacatc atgctgaccg tgtggtacat cttcttgatt tcgttccagc
    15061 ggtcgccgag gccgacggct ttggcctgct ggcgcaggtt ggagaactgg ccgcccggca
    15121 tttcatgctg gtagacttcc gtgttcggat acgcatcggc tttgtcgacg cccttatagt
    15181 acggacggac agtagcccag taacgggaca tttcttccat ggcatcgacg tcgaggtcgg
    15241 gctggcggtc cttgccgctc aaagcgtagt acaaggtgct catgctcggc tggctcgtgc
    15301 cgccggcaaa ggcggaataa gcgacgtcga cgatgtcgac accggcgtcg atggcgcggt
    15361 catacgtgca gatggcgttg cccgaaccgt cgtgcgtatg gagatggata ggcaggtcga
    15421 cggcatcttt cagggccgat accaggcggt aagcggcttc cggtttcaag aggccggcca
    15481 tatccttgat ggcgatgata ttggcgccgg cattctgcaa ttccttggcc atgttgacat
    15541 agtatttcag atcgtattta tcgcgggccg ggtcgaggat atcgccagtg tagcagaggg
    15601 cgacttcggc gagcttgttg ttttcgcgga cggcctggat cgtgccatac atgttgtcca
    15661 ggctgttgag gctgtcgaag acgcggaaga cgtcgatgcc gttcttggcc gacaggtcga
    15721 tgaagttctt gacgacgttg tcgggatagc ttgtatagcc gacggcattg gcaccgcgga
    15781 cgagcatctg caagaggatg ttcggcgctt tcttgcgcat ctggtgcagg cggacccacg
    15841 ggtcttcata gaggaagcgg taggctacgt cgaaggttgc gccgccccag cattcatagg
    15901 agaagaggtt cggcaatttc ttggaagccg cttccaggac gcggagcata tcgatggagc
    15961 ggacgcgcgt cgcgaacagg gactgatggg cgtcacgcat ggtcgtatcg gtaaagaaga
    16021 ctttcttctg gtctttgatc cactgggaga atttttccgg tcccatggca tcgaatttct
    16081 gcttcgtccc atccggatac gggccggcaa caggcgtcgg cagttccagg gcttcaaatt
    16141 ccggtttgtc ctgatggccg gcgtcggcat aaccgttgat ggtcgtattg ccaatgtatt
    16201 tgaggagctt cgtgccgcgg tcctggacga cgggcaggtt gaacagttcc ggatggtcgt
    16261 cgatgaagtt gacgttatac gtaccggcga tgaattccgg actcttgagg acgttgatca
    16321 agaagccgat gttggtcttg acgccgcgga tacggaattc cttgaggacg cggagcatct
    16381 tttcagctgc ttccttatgg gtccggccat aagtcgtcgt cttgaccagc agcgaatcgt
    16441 agtacggcgt gatgatggcg ccggtgtagg cattgccgct gtcgaggcgg acgccaaagc
    16501 cgccgccgct gcggtatacc atgatcttgc ccgaatcagg catgaagttg ttcatcgggt
    16561 cttccgtcgt gatacggcac tggatggcgt cgcccaggca gcggacgtcg tcctgggact
    16621 tgatgccgat ttcgtcgctg tcgagggcgt agccttcggc gactttgatc tgggtctgga
    16681 cgatgtcaat gcccgtgatc atttccgtaa cggtatgttc gacctgtacg cgcgggttga
    16741 cttcgatgaa gtagaattcg ccgtccggcg taaccaggaa ttcgaccgtc ccggcgttga
    16801 cgtaatggac gttcttcatc aatttcaggg cagcgttgca gattttctga cgcagttcga
    16861 caggcagggc cgaagccgga gccgtttcga cgactttctg atgacggcgc tggatggagc
    16921 agtcccgttc aaagagatgg atgacattgc cgtgttcgtc gcccatgatc tggacttcga
    16981 cgtgtttcgg attgacaatg catttttcca aataaatttc atcgttgcca aaagccagtt
    17041 tcgcttccga tttcgcgcgg tcgtaggcat cgcggagatc agccatgcgg tcgaccatac
    17101 gcataccgcg gccgccgccg ccgttgacag ctttcagcat gatcgggaag ccgacctgtt
    17161 tagcgaattt ttcgacttct gcataattca tgaccgggcc gtcgctgcca gggatgtact
    17221 ggataccggc cttcttcgcc tggatacggg cattgatctt atcgccgaac atgatgaggt
    17281 gttcaacttt aggaccgatg aagataatgc cttcttcttc acagcgtttg gccaggtccg
    17341 cattttcaga caagaagccg tagcccggat ggatggcatc gacgtcatgt tcatgagcaa
    17401 tacggataat atcttcaatg tctaaatatg catctaccgg ttttttgcct tcaccgacga
    17461 gatacgattc atctgcacgg aaacggtgca gggataacga atcttctttn nnnnnnnnnt
    17521 ctttggaata aatagctaca gtacggattc ccagttcatt acaagcccgg aaaacacgaa
    17581 tagcgatttc cccgcggttg gcaactaaaa ccgaacgaat ttttttcatg aaaataccct
    17641 cctgcttaga aaaattaagt caagaccggt aaacgcacag atgtccactc ccatacattc
    17701 accgctttct tcattataac aaaataagaa aacattacaa gggacggcgc actttgtctt
    17761 tataaaaata ctgtatacaa gtcatgtttc tccgcccttt tccccgtttc cccggcttct
    17821 gccgtttatc gccctcatga tgatgattgt tccttcatat aagacaatca tgggaacggc
    17881 aatacaggtc tgcgtaaaca tatccgtcgt cggcgacacg acaccggcaa agatgaaggc
    17941 caggataacg aagacgcggc gcttgccctt cagcatggcc accgtcacca ggcccaggcg
    18001 ggacaggacc agcaggacca aaggcagttc aaagcccagg gcaaagggca ggaggaaagc
    18061 gataaaaaag gataaatatg ccgacaaaga aaacataggc tgcaacgaca ccgtggcaaa
    18121 gtccatgaag aactggaccg ctgccggcag gacaaagaaa aaggcaaagg ccgatccgcc
    18181 atagaagaga agcagcgccg acgggatgaa ccacaaggcc gtcttcactt ccccgccgcg
    18241 cagggcaggc cgcaggaagc gccagaactc gaagcagtgg atgggcaggg tcccgatgaa
    18301 accggcaaag gcggcgatct ctatataaga aaaaaagact tctgccggat tgaggaaata
    18361 caatttcccg gcaggagccg aaatgacggc gataatatct tcagcatata tataggaaat
    18421 aatactgccc accagccagg ccacgaggca gacgatgatg cgccgccgca attcctccag
    18481 atggaaagta ataggagctc gttcttcaga catgggattt tcctttcagg tgtataaaag
    18541 ggcttgtcgt ataacgacaa gcccttttaa gttggtagta gttagttgtt agtggttagc
    18601 ggatggcttt aaatttaaac ctttctctgc aaactacaaa ctctgtacta caaactaatc
    18661 atacataatg tacgcgcgac gggtcgtagc ggtcttcgcg gggtttttct tcggccgtag
    18721 cctgaggata gcccatggcg acgatggcga acggggtcag gccctgggga acgccgaggg
    18781 accggcggac gaggacttcg cgggccttat acggggcgac gccgagccat acggcaccga
    18841 ggtcgaggtg tactgcttcg agcaggatgt tttcgacagc tgcgctgagg tcgaggttga
    18901 tgaaagccgg aaaacgcatg ttttcatcgc gggaacagac gacgatggcc aaggaagcac
    18961 cggcagcaca gccggcataa ggactgcagt gggacaggtc ctggatgacc cgtttgtcgc
    19021 ggacgacgta gaattcccag ggctgctggt tcccagccga aggagcggcc atagcggcac
    19081 gcagtaactg ttctatcttt tcatcttcta ccatggtcgg taaaaattta cggatactcc
    19141 gtctggcaaa gatttcgttc atgtgaatcg actccctcct ataatcatat atctatctca
    19201 ttataacata tatagataga aagaaaaaga agttgtatag gcttccatca cgaatgatga
    19261 aaaagaggct tgccgctatg cgacaagcct ctttgcttgg tgccgaggga gggacttgaa
    19321 ccctcacggt gttacccgcc tgattttgag tcaggtgcgt ctgccattcc gccaccccgg
    19381 catatctctt gtgcccggca catggtgttt cagtgcttgg cacaagaaga attataccat
    19441 aactaacgga atcgtcaagt attttttttg aaaattttta cgacttaatg gccagccggt
    19501 actttttctt ccgaatcttt ggctacgacc atttcttctt ctttcaaatg agcgaagtat
    19561 ttcttcgttt cggctacaac gacgccagac aggccgatga gggcgatgag gttcgggata
    19621 gccatgaggc cgttgacgat atcagccagg acccagatga cgtcgagttt gatgaaagcg
    19681 ccgcaggcga cgagggcgat gaagatgatg cggtacggca tgataccttt gacgccgacg
    19741 aggtattcga cgcagcgttc accgtagtag ttccagccga ggatggtcgt gaaggcgaac
    19801 aaggtcaggc cgacgcagag gatgatgctg ccgtaggtcg ggaaagccat ggtgaaagcc
    19861 gcattggtca gggctgcgcc gttgacatcg cccatccagg caccgctgac gaccaggacc
    19921 aaaccggtca aagtgcagat gatgatggtg tcgatgaagg taccggtcat ggaaatgagg
    19981 ccctgttcag ccggccattt cgttttggca gcagcagcta cgataggtgc actgcccagg
    20041 cccgattcat tggagaagac gccgcgggca acgccgttgc gcatagcaac caataccgtg
    20101 gcaccgagga agccgccagc tgcagccgtc ggtgtgaagg cgctgacgat gatctgttca
    20161 acagcagccg ggatctggtc ggcaaagacg acgaggaaag cgaccgtaga aacgacatag
    20221 atgacagcca tggccgggac gattttggaa gcgacgcggg cgatggactg gaggccgccg
    20281 atggtgatgg cagcgacgag gatagtcaat acgatggccg tgtattcgac ggggatgctc
    20341 gtcgtcaggc gcgtgatttc aacgatggaa ttgacctgcg cgaaagtccc gatgccgaag
    20401 aaggcgacga ggacgccgaa gaaggcgaac aagaaagcca agggcttata tttcttgccc
    20461 aggccgtttt cgatgtagta catcgggccg ccggcaatat cgccgttctt gtcgaccgtg
    20521 cgatatttga cggccagaca gccttcggcg tatttcgtgg ccatgccgac gaaagcggcc
    20581 atccacatcc agaagatagc gcccgggccg ccggcatgga cggccgtagc gacaccgacg
    20641 atgttgcctg tcccgaccgt agcggccagg gccgtgcaca gggccttgaa gctgtctacg
    20701 tcgccttcgc cctggttctt ggccgtgaag atgagcttca gcgctctcgg caggcggaag
    20761 acctggagca aatggaggcg gacagtcagc atcaggccgg tgccgatgag cagcaccagc
    20821 aagggcggac cccagacaaa accatcaatg gcattcaacg tttctaacat ctttcatact
    20881 ccccttttgt tatggtaatt ataacgacat gaaaaaagac ctatcatcat cactctgaag
    20941 ataggtccta agggtcgcag aaaaagaaag gtcgttctgc cagctctgtc cttttgcctg
    21001 agagattagg aatcgcttcc ttgcaccttc ggcgcccaaa cgggactctc cagagttgtg
    21061 tccacacacg gtcccacccc tttgcggggg cacctgagag tttgactccg tcggtaggca
    21121 ttgccttctc ccgtgtgttt cgttcacgca atatgtatga tgtgattgtt attataaacc
    21181 ggcttcttaa aaaatgcaag ccctttacgg aaatttgtcc gtattcagcg aactttcttt
    21241 ataaatctgt gtcagaaaag gcgtacggca ggagctgatc catcgtgacg acgcaccatt
    21301 cccctttcag gttggccatg atgaccttgg ggatatggaa ttcggccatg acctgccggc
    21361 aggcgccgca gggcgaacag gggccgggcg tatcggcgac gatggcaata gccgtaaagt
    21421 cccgctcccc ttcggatacg gccttgaaga tagccgtccg ttcggcacag ttgcccaggg
    21481 gataactgga attctcgata ttgcagccgc catagacctt ccccgacgcg gccaggacgg
    21541 cggcgccgac gggaaaatga gaatacgggc tgtacgaatg agcgcggtag gctttcgctt
    21601 tttctatgag ttcttcatca gtcatagtga cagacatcct tttaagtggt ccatgacccg
    21661 gtcggccgtc tgctgccatt tgagcggcgt cagggcgata tgaccctggc ggatgcaggc
    21721 gacatcgctg tcgtcgggag ccgtcgtcat gtcgatatcg ccgacgatac gatagcagac
    21781 gctgccgtcg aggtcgcgtt tctcagcgat gacattgtgg taaatctgga ccgtctgggg
    21841 cacgacgcgg acgttgtcca ggctgacagc cccttctggc ggcacgttga tgttggcgat
    21901 gcccgggaag cggcctttga cgacgagctt ctggaccagg tcccagacga ggtgggccgt
    21961 ctgagcgcag cgttcatcgg tcatgcccag catggatacg gcaaaggccg gcaggccgta
    22021 atacggccct tccatggccg ccgacacggt cccggaatag aggacgtcgc tgcccaggtt
    22081 atagccgtcg ttgatgccgg aaatgatgag gtccggttta tcttctttga ggaaatattc
    22141 catagccatc ttgacgcagt ccacaggcgt accggatacg gcgatttccc ggattccctg
    22201 taccatcgat tcgtcgcggc agtagagggc cttgttgatg gtcatggaat gagacgccgc
    22261 actgcgctgg ccattgggcg caacgacagt caggcggcag tcatgagatg ccagttcccg
    22321 ttttagcgtc tgaagacccg gcgctttcaa tccgtcatca ttggtcaaca aaatatgcat
    22381 agtctatctc cttacttctt attttcttta cgcaataagg ccgcgctttc ataagacagg
    22441 gcccgcgtgt gcagggtgtg gctccatttc atcttgtgcc ggtctttgaa accgaggaag
    22501 ttgacaggga tccagctgta catgaaaatc ggataaataa tgaggtatgc ccatgatttc
    22561 ggcggcacct tgatctgcaa gaggacgatg accggaatga ggtactggcc gacgccgatg
    22621 atggtccaaa tctggacggg cacgatggca ttgaggatat tcgtatagaa gggcaggtac
    22681 gaattaatcg aagccaggac ggcgtaaatg gtcgaagcca tcatgaaaaa tggctgggac
    22741 aaggtgacga tgccgtcgag catgcggatg ttgccggtct tgataccccg ggcgaagagc
    22801 ttgggaatga agcgttcgcc gcagtcacac tggccctggg cccaacgctt gcgctggcgg
    22861 caggactgca tgaagccgac gaccttttcg tcgtaaatga tggcatcatg ggcccagcag
    22921 gtgcggacgc cttcgaggag gactttcatg gaaaattcca tgtcttccgt caggcagtca
    22981 cagccccagc cgtacttgcg gacgatttcc gtggcgatgc acatgcccgt gccgcccagg
    23041 gccgtagaca agccgatgtt gtacttggcc aggtgccaca tgtggttgat gagccagaag
    23101 gcgatggaaa aggtgccggc aatccacgag tccgtcggat tcttggaatc catatagccc
    23161 tggatgacct tttccccctt gcagaggtgg ttgttcattt cccgcaggaa ttccggatgg
    23221 accaggttgt ccgcatcgaa gacgacgaag gcgtcatact gcttctcctg ggccagcatc
    23281 tgcgagaaca tccagtccag ggcatagccc ttgccgacct gttccttgtt ggtccgcacc
    23341 ttgacgatgg ccccgtgctc gcggcagatg tcggccgtat ggtccgtgca gttgtcggcg
    23401 acgacgtaga tgtcgtacat atccttcgga tagtggagct gtttcagatt gtcgatgagc
    23461 tgccagacga ctttttcttc attatgcgcg caaataacgg cagcgaaggt attcttaggc
    23521 gctaccgtaa tgcgttcccg ccgcttcatg aggccgaagc aggcgaggat gaaataatag
    23581 agggaataaa aaacgatgat gacctgtatg ggtatcatga tgatatccaa gatgtgttcc
    23641 atatgcgact ctccctaaag acttagcgag cagctgcgac gttcatggcc gtattgacta
    23701 acccgaagac gatatacagt aagaaaggca tggtgatgat cagatggaag tcgacgaaga
    23761 ccaggaccag aacgccgaag atgagggaaa tggccagggc cgatttgtgg agcacgtccg
    23821 ggctggcccc tttgaaatcg gggttatgga ccttgctgac cagatggtag cccaggagga
    23881 cgacgaagat ggccagcagc catgccggca ggacggcgcc ggaaatgacg tacgtcgcaa
    23941 tgaggcagcc gccattagga atcggcatcc cctggaaata gccgtggatg acgccggtgt
    24001 tgaggttgaa ccgggccagg cggataccgc cgagagcggc gtagatgatg gccgggatgg
    24061 cgccgatcca gcccagggac tggagtgaat aagaatagat caggaaagcc gaagcggccc
    24121 caaaggatac gacgtcggat aaggagtcga gttccttgcc gaaatcaccg gcgacaccaa
    24181 gggcgcgggc cgagcggccg tcgcaggcgt cgcagaccat ggccagcagg atgcagacac
    24241 cggcccaggt atagagcccg tggagggtca taatcatact caggaccccg aagctgaggt
    24301 ttcctgacgt aaacaaacat ggtattacat ttttcattaa actatcctcc ctatgacgga
    24361 tttaccagca cggaccttct ggcccttctt gacggtgatt tccacattgt cgcggacata
    24421 gatttctgca cacgacccga atttgatcat gccgtagagc tggccgtgct gcagttcatc
    24481 gcccaaggtg acccaggaga cgatgcgccg cgccaggatg ccggcaatga tggtgaccag
    24541 gatatccgta tgggctgaat ggatgccgat ggaatagcgt tcattttcat agccgacgcc
    24601 ttctttataa gccggacgga agcggccgca cgtatactgc tggaaatcaa tggtccccgg
    24661 cagcggtgcc cggttgacat gggcatcgaa gatggacagg aaaatgatga ttttccggca
    24721 cttctggttg agataggcat cttcttccac ttcttcgacg ccgacgacag tcccgtcagc
    24781 cggcgacacc aggagatggt cgtctgtcgg catggtccgg ctggggttgc ggaagaaata
    24841 ggtgaaatac gcagccagca gcagcagcgg aaccacggcg taggcattga tgaaatagcc
    24901 tgctgccaca gccagtaaca gaggcacgat aatgaagggg tatccgtctt ctacgatata
    24961 aggtttgttc aagacatcac ttttcaactc atcacctcat cacttaggcc ggccgcccag
    25021 cttgacagcc gcgacgaatt tcggcagcgc cagcatgcgt ttgaaccgcg tcggctcctt
    25081 caacaggcga tagagccatt ccaggcggtt gtcctgcatc catttcggag cccggccggc
    25141 tttgccagcc aggacgtcga aggtcccgcc gacgcccata cagacgcagg ggccgagttc
    25201 gtagagatgc tcagatatcc atttttcctg tttcggcacg ccgagggcga ccaagaggat
    25261 gcgcgtcccc ttgctgcgga tatcttcaat gataccctgt tcttcgtcag ccgaaaagaa
    25321 tcccgaatga gtcccgacga tgttcaggga ccccagttgc tgctgcacat tggccgcagc
    25381 ctgttcggca atgcccgggg caccgccaaa gaggtagaca gccgtttggc gtgcggctgc
    25441 ttcttggagc agactgcacg ccaaatcgac acctgtcacg cgttctttaa atttcttgcc
    25501 ctgctgttct gcagcccaca ggacgccggc cccatcggga actaccaggt cggcatgctg
    25561 gaggatatgg gccagttcgc ggtccgtctg ggcccgcatg accatttctg cattggccgt
    25621 cgcaatggaa gcggcccggc cttcgtcgat gcacttaaag gccaggttcc gggcttcttc
    25681 catagtgacg ttccatatgg ggatggacaa gatatcgatt gtactaatca acgtatccct
    25741 ccgccctgct tagtttacgc tgtagttcgg agcttctttc gtgatgttga catcgtgagg
    25801 atggctttca cgaagaccgg ctgcggtgat ctggatgaac tgcgtatttt caatcatttc
    25861 tttaatgttg tggcagccac agtagcccat ggatgcgcgg agaccgccga ccatctggta
    25921 aatcgtgtca gctgccggtc ctttgtacgg tacacggcct tcgatgcctt cggggacgag
    25981 cttcttggca tcttcctgga agtaacggtc cttactgcca cattccatag cggccaggga
    26041 acccatgccg cggtattctt tgtagctgcg gccctgatag atgaccgttt cgcccgggct
    26101 ttcttccgtg ccggccagga ggttgcccat catgacgacg ttgccgccgg cagcgatggc
    26161 tttggccata tcaccggaat atttgatgcc gccgtcggcg atgatgggga taccatagcg
    26221 ctgagcgact ttagcacatt catagacggc cgtgatctgc ggtacgccga taccagcgac
    26281 gatacgggtc gtgcagatgg agccagggcc gatgccgact ttgacggcgt cgacgccgca
    26341 ttcgatgagg gcttccgtag cggcacccgt agcgacgttg ccggcgatga ccgggagatg
    26401 cgggtacatc ttcttgattt ctttcagcgt attgaggacg cccagggaat ggccgtgagc
    26461 cgtatcgatg acgacgacgt cgactttggc agcgacgaga gcgtcgagac ggtcggtcat
    26521 gtcatgggtt acgccgacag cagcagcgac caggagacgg ccgttgccat ctttggcaga
    26581 attcgggtat ttcgtagcct tttcgatatc cttgatggtg atgaggccct ggaggttgcc
    26641 ttctttatcg acaaggggta atttttcaat gcggtgcttg cggagaattt ctttggcttc
    26701 tgccagggac gtgccgacag gagcagtgat caggttttct ttggtcatga tgtcaccgat
    26761 gcggcggctc atgtcttctt caaagcgtat atcgcggttg gtgatgatgc cgacgagttt
    26821 gccgtcgacc gtgataggta cgccggagat gcggtatttg cccattaatt cattggcatc
    26881 ggccaggaga ttatcgggat gaaggaaaat cgggtcgatg atgatgccgt gttccgaccg
    26941 ctttactttg tcgacttccc gcgcctgggc ggcaatggac atgttcttat gaataacgcc
    27001 gatgccgcct tcgcgggcta cggcaatagc cataggagct tctgtaacgg tatccatacc
    27061 agagctcata ataggaatat tcagtttgat atcgcgcgtc aggttcgtcg aaacgtcgac
    27121 ttccttcggg agtacgtccg attttgccgg aaccaggaga acgtcatcaa atgtcaaacc
    27181 tctcatacca aatttatcag atctcatatt tgcacctctg ttttgaaaaa tataagaaac
    27241 taaggggaaa tacccctaaa tagcttatcc cctattataa ctgaaatcag ctcttatgta
    27301 catatattac nnnnnnnnnn ggtcttcgtg gcccagccac cattgccggg ccgcatcgcg
    27361 ggccgccgcc aggagaggta cgtcgtttat aatatcggca gctttcaaat cgggcaggcc
    27421 atgctgcata tagccgaaga gctgtcccga accgcgcagg agcaggtctt tttcagccag
    27481 cgtgaagccg tcgtgtatct gttccatcca tttcaggcgc tgccgcgtct catcgctgcc
    27541 gcctttggcc atgaggatgc aataggcctg cagatcgccg cggccgacgc ggccccgcag
    27601 ctggtggagc tgggccaggc cgaagcgttc ggcgccgtcg atgaacatga ccgtcgcatt
    27661 ggggacgttg acgccgactt cgatgacgct cgtcgcgacc aggagctgga tatccccttt
    27721 ctgaaaggcc tccatgacgg cttctttctc tttgccggcc atacggccgt ggacgaggcc
    27781 gcacgtgcgc tttttgaaga cgcgggaccg cagttcttcg tagaggtccg tcgccgcctg
    27841 caaatcgacg ctttccgagg cctcgacgag gggacagacg acgtagcact gctggccctg
    27901 ggccatgagc ttgtccatga agatatagac ccgccggcgc agggcttcgc cgacggcata
    27961 ggtcttgacc cgtttccgcc ctggcggcag ctcgcggatg gacgacacgt cgaggtcgcc
    28021 gtagagggac aaggtcagcg tccggggaat gggcgtggcc gtcatgaaca gggcatgtgg
    28081 ctccttcccc ttttgttcca gcgccgaccg ctggcggacg ccgaagcggt gctgttcgtc
    28141 cgtgacgacc aggcccaggc agcggaaggc cacgtcgtcc tgtatcaggg cgtgagtccc
    28201 gatgaggatg tcgatgcgcc cctgggccag ttcttccagg agctgctgcc gttccttagc
    28261 cgtcgtccgg cccgtcagca aggcgatgtg gacgtccagg ccctgaaaga ggcggcagaa
    28321 ttcttcatag tgctgggccg ccaggatgcc cgtcggcgcc atgagggctc cttgatagcc
    28381 gttctctacg atcttggcca gggccagggc ggcgacgacg gtcttgccgg agccgacatc
    28441 gccctggacc aggcgctgca tgggcacttc gctctccata tcggcctgga tatcgaggaa
    28501 ggcctgggtc tggcctttcg tcagggtaaa gggcaggtgg tcatagaagg ccttgagcag
    28561 atggccgtta gggccgcatt tgatgccctg gcgcttgcct tcatggcgcc ggcgcaggag
    28621 caggccgagc tgcatgtcga agagctcttc aaaggccagc tggcgccggg cctgttcccg
    28681 tttctccata gaatcgggga aatgcatctg ctcataggct tcgagcgtcg gcatggcggc
    28741 aaaaggcgac tgacggcagc cgtcccagcc gggcaggatg tccggatgtt ccctggccag
    28801 gtccagggcc tggcgcacgg ccttgcggat atcctgctgg cgcaggccgt cggtcaggcc
    28861 gtagacgggg acgaagccat cgccgccagc ctgcccttct tccgtgtcgg gcgtattcat
    28921 ctggcggcgg ccgtaagccc attccatctt gccgaaagcc gtcagggtca ggccgacatg
    28981 gaactggcgt ttcttgaagg cctgattgaa ccagaccagt tcggccgccc ccgtgccgtc
    29041 gctgaccatg accttcaaga tggagaggcc ccggcggggc cgcatttcct ggacagaggc
    29101 cacggtcccg cagaccagga cggctttccc cgtcccgcac ggcgcctggc cgatgggcca
    29161 gatgtggctg cggtcttcat aatcgcgggg catatattgt aataaatcgg acaccgtcgt
    29221 cagatggagc cgggcaaaca gggcttcttt gcgcggtccg atgcctttta aatcgcgaat
    29281 ggaaaaattc ataccaaaaa ctccttgtca aatattcgtt tttattgtat catatttcgt
    29341 aggctttttt aaaaaataaa acttgctttg cctaataatc tgtgataaga tttatatgtt
    29401 gtactgtgat ggtgcaggga ggtgtaacca atggcaaact tttgcgaaat ctgtggtaaa
    29461 ggaacaatga gcggcatgaa tgtcagccat tcccacttga aaacgaaaaa aacctggaaa
    29521 ccgaatatcc agcgtgtccg cgctgtcgtc gacggcgaag taaaacgagt taatgtttgc
    29581 actcgttgcc ttcgttccgg taaagtccag cgtgatgttt aaggatgata aaaaagaggc
    29641 tgtcacatta agacagcctc ttttttagtt taaagtttgt agtttttagt ttgcagtgaa
    29701 tgtttaaaaa tttaaagctg tcatctgcaa actacaaact ccgtactaca aactacaaag
    29761 agaaagggac ctcgcatgat agaataaacc ttcatgcgac agtccctttt ttcgtggttc
    29821 gtagctcgta gggggcgcca cgtgggcgac ccgttgggca tttgtgtgat ttccccaacg
    29881 aaatgaacgg ggaatcgcac atatcctgga g
//
LOCUS       DAWABZ010000007        29068 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_465_length_29068_cov_3.164512, whole genome shotgun sequence.
ACCESSION   DAWABZ010000007 DAWABZ010000000
VERSION     DAWABZ010000007.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 29068)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 29068)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..29068
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_465_length_29068_cov_3.164512"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1570..1579
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6686 a         8179 c         8084 g         6109 t
ORIGIN      
        1 gtcagcttgc gctgacagct ctcctatgag gagagccatt gggaaagcct cccctcatag
       61 gggaggtggc cgctaggccg gagaggttga agttcaatat attttaaaag gaggttctat
      121 gaaaacgctt ccctattcta aagaaaatat atcatttgcc cgtttattaa ggaaaaatat
      181 gaccaaagaa gaacgtcact tatggtatga ttttttacgg acgtatccag tgaaatttta
      241 taaacaacga cgaattggta attatattgc cgatttctat tgcaaagaag cagggctcat
      301 cattgaatta gacggtgggc agcactatga tgaagccggt atgaagtcag atcaggaacg
      361 gacagctgta ttggaattct atggattgac ggtcatccgt tttaccaatt tggatatcca
      421 taaaaatttt agcggagttt gccaaactat tcatcaatac gtcagtgagc ataaaggctg
      481 ttcctggtga ttaaaaagca acctctcagt cagcctgcgc tgacagctct cctatgtgga
      541 gagccattgg gaaagcctcc cctcataggg gaggtggtcg ccaggccgga gaagttgtct
      601 ttcaaattct cctttttttg tgataaaata aaagaaaaaa gcaggtgata atgtgaagag
      661 aatatggctc atccgtcatg gcgaaagtat tgccaatgcc ggcgaagcga cgcaggatca
      721 tcgcaatatt cccttgtcgg aattggggct caagcaggcg caggccttgg ctttacagat
      781 tcctaagaaa ccggacctga ttgtcacgtc gccttatctt cgggcgcagc agacggccat
      841 gtgtacaata gggcggtttc ctgatacggt gacggggatt tgggactgtg tccatgaatt
      901 tgtttatttg gcaccggcga cgtgtgtcgg gacgacgtcg cagcagcggc ggccgcgggt
      961 catcaattat tggcgcaaac tcgacccgga ctatgtcgac ggcgaagggg ccgaaagtta
     1021 tcgccagctc atcgggcgaa tcgaacggac cttatctctg ctgcagcggc ggccggagca
     1081 attcatcctg gtctttacgc acgcccagtt tatccgcaac ctgctcttgg tctatgaaaa
     1141 tccgggcagg cctgtagaag aatacatggc cgctttccgc cgcagccgcc cggtccataa
     1201 tggacagatt gttgaaatta cgatgtaaag gagaaattac catggaatta caatatggac
     1261 ccaataaaat ctggatggaa aatgaagctg gaacggtcgt agctgaagtg gacttcgtcg
     1321 aacgggaaga cggcaattac gacatcgtcc acacctttgt cgacgacagc ctgcgcggcc
     1381 aaggtgccgc tggcaaactc gtcaaagccg cagctgacga aatacggcgg cgccataaaa
     1441 aaacggccac cagttgctcc tacgccgacg cctggttccg ccgccacaaa gacgaacagg
     1501 atttacttgg ataatcagga cgagaatcca cttgatttat ctacattcat gatagaatga
     1561 gaatagagcn nnnnnnnnnc ctctagtttt agtgtttgaa gaaggccgtg taccacaagt
     1621 acgcggtttt tttcatatta caagtttatc gcttgcttaa cactcgaaaa aagctgcctt
     1681 cgtatggcag cttttttctt actgaaggac tttcacccgt tcttcgatgg ggacttttac
     1741 gatgggtttt ggcggttctg tgggatggcc gaagggcatc tgggcgatga gctgccattc
     1801 tttgggaacg cctgtgagtt ttttgacttc gtcgtcgatg agggggttgt agtgctggag
     1861 gttgacgccg agtcccaggt cggtcagggc ggtccagacg gcgaactgca tcatgccgtt
     1921 ggcctgctgg gcccagatgg ggaagttctt ggcgtagagc gggaactggg aggccatgtt
     1981 gttgacgatg gtcgtgtcgt cgaagtagag gatcgtgcca taggctgcgg cgaaggagtt
     2041 gatcttggct tccgtcgtgg cgaagttggc tggcgggacg attttgcgga gcgtatccat
     2101 ggtgattttc cagatggcgt cgtgcgtttc gccaaaggcg atgacggctt tgccggactg
     2161 catgttgaag gccgacggta cttcgcggat gacgtcttcg atagtcttgg taatggtttc
     2221 ctggggaatc gtgacgtctt tgctgagggt gtagttcgtg cgccggttgc tgacggctgt
     2281 gatgaagttg gtcataagag cacctcctga atttacaaat catttcctaa ttgatagtaa
     2341 ttattagttg tttattggct aaagtatacc atagtgaaga aaagaagtca agaatcatga
     2401 ccccttgaca gcaaggcttt tttcatgata ttatttatac attgcgtaag caaaatttaa
     2461 tttcttgaat tactaatatg aggtgatgta gaaaatggca acgaggagag acccgcgttt
     2521 taaggaatgt cgccggttcg gagttaatgt gtacggacac cctaaggcat taaaacgcgt
     2581 acgtaaagat cagcgtcaga aaaaaatgtc tgaatatggc acacagttat tggaaaaaca
     2641 gaaacttcgc gcatactaca atttgctcga acgtcagttt gtccgttatt atgataaagc
     2701 caagaagatg gaaggcgtta ctggccagaa catgctgaaa ttgctggaat gccgtttgga
     2761 taacctggta taccgtatcg gttttgcaaa ctccatccgc caggcacgtc agatggtcaa
     2821 ccacggccac atcctggtca atggcaaacg ctgcgacatc ccgtcggcac acgttaaagt
     2881 tggttccgtt atcgctttga aagaagcttc ccgttccaac gaaatgtaca aaaaatcttt
     2941 ccaggaattg aagactttcg atgtaccgta catcgaaaag aacttcgacg gcttcgaagg
     3001 cacacttacc cgtatgcccg aacgcgacga aattccggta gaaatcaacg aaacacttat
     3061 cgtcgaattg tactccaaat aagagctgtt tgatggtaac aaaaaggcgc tgtcgcatga
     3121 agacagcgcc tttttttggg ggttagcagt tagtggctag ctgatgattt tataaaacaa
     3181 gggactacgc atggtgatat gagactgcat gcgatagtcc cctttttttg tttgatgtta
     3241 aaggcaacct ctcagaccgc cttcggcggc cagctcccct ataatgggag cctttcgtca
     3301 aacattcatg tcacgagcca cgagccacga gccacgaacc actagccact aacttaccgt
     3361 ccttcagaga agtccaggcc tttgtcggtg gcgtagtatg tgcctggttt ttcgccggct
     3421 tctacgtagc ctttttgtac caggtcgtcg atgagggccg ggtccggtgt tccgggagcg
     3481 aacatttctt tcggccttcc ttcttcgcgt tcgacggcgg cgatgagggc ggcttctttg
     3541 tcgtctgtcg tcggtgcttt ttggacgtac gtcaggtcgg gcaggaacat ggggtattcc
     3601 ttgtgggcca ggagaaagaa gcggcctatg gcgatggcct ggctcaacag gttgccttct
     3661 tcggtctgga tatccatttc atcgcagatg acgtcgagct ggttcgacgg gaagtgatag
     3721 agtttcttgg cgacttccag tgtatcggca tagcagaagg ccggtgcgtc gatgccgtag
     3781 atgtgcaggg cgtgggtcag gcagcccatg acgaagggcg cgttgtgggc gaccagggtc
     3841 tgactttcga tgaggttttt caggccggcc tgccagtatt cgccgaattc atcggccttg
     3901 gagagttcgt cccactgata gcggcctttg acggcagaca agttgttttc tggcggacgg
     3961 atgtagaagt aacgcgtatc cttcagttcg ccgtggtcga attcagccag ggccaggaga
     4021 cagacggaat agggctgttc gttggccagt tccagggcga cggccgtgaa gtgctgcggg
     4081 aagcggcgca gtttcccttt ttcttcgatg ggatatttgt aatagccgtc attgggcttg
     4141 atgtcgtact ggtgcaggtt ggcgactttg acgtgttcct tcttggccag ttcctgctgg
     4201 accttcgcct tgcgatggcg cttgcgcagg gccgacgtga gggaatagag gacggcagcg
     4261 atgaagacga tagtgacgat cgtcgtaatg atgtggatca gggaatccat ggaatatcaa
     4321 atccttttca ataatttttg agccttattc ttggaagaaa ttcatgtcgt aacaggcccg
     4381 gaaagcgcgg ttggccgcat tgccgttggc agacaggcgg tctgtcgtct tgacttccgg
     4441 attgggcagg gacaaggtgg acacgacgtt gccctgcaaa tcgtagccga catcgccgag
     4501 agtctgtgtt tttacggtct gggctttaaa attgtagaag aaattgttat cataaccttg
     4561 gaccgtgttg ttggcatagt cataggtcac gaccgtagcc cggatgatgt agtacggcgg
     4621 atcgtagcgg acggactgga tggtgttcat gtcgatatac gattcggtcg tgtcatcgtg
     4681 gccgacctgg acgaagcggg ggttgtcatc gagaaatgtg gcggatgcca tgaaactgaa
     4741 gacgcaggca aatgtgactg ccagagcggc aatttttttg aacataagac atacttcctt
     4801 tccctgtctc ttccaatcga ggcagtttat acatatatta tatcggaatc atgaaacatt
     4861 acaagaacgt atatttttca agtaggactt ttgccctttc gggtcgaata ataataatat
     4921 aagatagaag aggtgatcat attggatgtg atatatattg gcctgccttt cttcttttgg
     4981 caggaagatg agtcggagca cggcctcgat gtccacgtga cggaaggctt ccagaagctg
     5041 ggttcccacg tgtaccccct caatgccggc gacgacgcgg aagaaatctg ttcggcttat
     5101 aattggcata catcctttgt cgatgaagac gaagacatta cgccgtcgga agaattcatt
     5161 tcagaacatg tcttgtggga cgatttccgc ctgctctata tttcggcagc tgcggctacg
     5221 tcggaagacg aatacacgca gttcgtctgt catacggcag agttcgccaa agagagcggc
     5281 ctcgtcgtgg cagcggaagt cgtcgacggc gacagcgacg atgaagaccc gtatccctgg
     5341 cgcgataagg cgacggtcct ctggtcccgc agcgatatcc tgccatcggg aggtccggcc
     5401 tgtgccgtgc gcctggccct gggcaggggc atcaccattg ccagccaggg cgaagaacgg
     5461 acctatgacg tccaggtcgt cagtgaatgt ttcatccctt cgtttttaca aggattgctg
     5521 gaagggcgcg atcccttttc catcatagaa tcttatgtat cttaagttag gaggttattt
     5581 atcttgaaca gtaaaaaaat ggaagaacat cctttgcggg cggccattct cactatcagc
     5641 aacagccgca ctttttcaga cgatacgaac ggcctgctca tccagtcggc cctggccaat
     5701 tacggtcatc aggtcgtcga ctatcagatc gtcaaagacg acaaggccga aatcgaacgc
     5761 catctccatg aatgggtctg cagcgtcgac ctggtcatca ccagcggcgg tacgggcatg
     5821 gccaagcgcg acgtgaccat cgaaacggta gaagccctgt tcgataagaa aatcccggct
     5881 ttccagtcga tgatgacgta tttcgcctat cgccgggcct gcggcgtcca ggccatggcc
     5941 taccggacga cggccggtat catccaccag tgccaggtct actgcctgcc gggcctgccg
     6001 agcctcatca agatcggcat ggaaaaggtc attttgccgg aagcctttca tttgtattcg
     6061 gaaatcaaga agtaaatgga tacgtctaaa atcatacttg tcaccggcgg ggcgcggagc
     6121 ggtaagagcg cctttgccga acgctatgct gccgcccttc ccgggcggca tgcttatgtc
     6181 gcgacggccc gcatcttcga tgaagaaatg gcccagcgca tcgccatcca ccgcaagcgc
     6241 cgcccgtcgt cgtggcagac ctgggaaatc ccgcaggacc tgccagagac gatggaacgg
     6301 ctgtgccagt cgtcggacgt ggccctcgtc gactgcctga ccctctattt ttctaattat
     6361 ctctttgccc acgacggcga agacgacgaa gctatcatcg acggtgccct ggccgaactg
     6421 caagcggtcc tggcggcttt ccgccaggcc ggcgtgacgg ccgtcctggt cactaacgag
     6481 ctgggctgcg gcatcgtgcc catggaacac gtgagccgcc tgtatcgcga cctcatgggc
     6541 aagatcaacc aggccgctgc ggccgaagcc gatgaagttt acttatcggt ctgcggtatt
     6601 acgacggaac tgaagcatca ggccgtctgc ctgccggagg tgaagtcatg aaggcctttc
     6661 tcattgccct gcagttctta tcgcgcatcc acctggcttc gcagagcgtc tggcgcgatg
     6721 aggatttcgg ccggtccgtc ctgttctttc ccctcgtcgg cttgatcatc ggcctgcttt
     6781 tggcggtgct ctattgggcg tcgtctttcc tttttgacgc ctttggccgg tcggccctgg
     6841 tcgtcgcctt ctggttctac ctgaccggcg gcctccacgc cgacggctat atggatacgg
     6901 ctgatggcct cttttccggc cgcagccgcg agcggatgct ggaaatcctc aaggacagcc
     6961 gcgtcggcgc cggcggtgtg acggctttcg tcttcctggt cctcttgaag gtagccttcc
     7021 tcagtcagct ggcaccggaa gcagccctgc cggccctcgt cggtatcccg gcagcggccc
     7081 gcttcgggac tttgatcagc atcttccaat ttccctatgc ccgcgagcag gggctgggcc
     7141 gggccttcgt cacttacgca ccggctcata ctgtagaaaa agccctggtc ctggccctgg
     7201 tgccggtctt tttctgcggg cccttttatc tggccctctt aggtgcggcc atgttcctct
     7261 ctttgtgggc caacagccgc atcagccgca agctgggcgg cgtcacgggc gatacgtatg
     7321 gcgccgtcct cgagggcagc gaaatggccc tgctcctgat gacggccgtc ggcagcaagc
     7381 tggccgattt cttgttataa tgggacggca cgaatgttga attacttata gggaacttac
     7441 ggaggattac gatgataaaa ttatatttag cacgtcatgg tgaaacagaa ggcaatgtcc
     7501 agcagtggta tcaggggtcg accgacgtcc ccctcaatga acacggcctg gaacaggcca
     7561 ggtgcctggg tgaatttttc cgccatgtcc atctcgatgc cgtctacagc agcacgctcc
     7621 agcgcgccaa ggtgacggcc gaatgtgtcg ctgcgccgca tcatctggat gtcgtcgctt
     7681 acgatgaatt gaaggaagcc gatttcggcg cctgggaagg ccatacgtat caggaaatca
     7741 cgactcagtg gccaggcgaa ctggaagcgg tctatgcgtc ggacgggacc ttgccggccc
     7801 ggggcggcga atcgttctgc caggtccggg accggacatt gaagaagacg cgggaaatcc
     7861 tgtcgcatca caaggacggc gacagcgtct tcatggtatc ccacggggcg gccatccgct
     7921 gcctgctctt cggcctgctg ggcctcgata tgaagcgcat ctggtgcttc cagcagttca
     7981 atacagcttt taacatcatc gaatattacg gcgaccgcaa tgtcatgacc ctcatgaact
     8041 gcacgcagca tctggaaggc ctgacggggt accagcccca gtgggacaag atgccgtcgt
     8101 tataaggagg catcatggca aagacaatgc gcctcgacaa gctcctgggc catacgggct
     8161 gggggacgcg gcgggaactc aaggaattgt gcaaaggcgg gcacgtgact gtcaatggcg
     8221 ccgtctgccg cgacagcagc cagaaaatcg acccggcagc cgatgccgtt gccgtcgatg
     8281 gtcaggccgt ccattatgaa gagtctattt atctcatgct ctacaagccg gccggcgtcg
     8341 tatcggctac ggaagacaac gtgtccccga cggtcatcgg cctcttgccg cgccagtacc
     8401 agggggccgg cctctttccc gtaggccgcc tcgacaagga tacgacgggc ctcctgctca
     8461 tcaccaatga cgggacctgg gctcatggca ttacgtcacc gaagaagcat accgataaga
     8521 tatacgacgc cgtcgtcgaa ggggatatcc ctgaagatgt aggggaccgc tttgccagcg
     8581 gcatcgtcct cgacgatggc ctgcactgcc tgccggccaa agctgtccag accggccccc
     8641 ggtccctgac ggtcgtcgtc caggaaggga agttccatca ggtcaagcgc atgtgtgccg
     8701 ccgtcggcct gaaagtcgtc cagctgcacc gccgctccat cggcagcgtc gtcctcgatg
     8761 acggtttgga gccaggccag ttccggcccc tgaccgatga agaacgggaa gcgttgcgca
     8821 gccagagccg gtgatttgca attttatctt ttcagccagt ggaaaatatg gtattataaa
     8881 atatgattga attactatag ggtgataggg ggatactatg atagaacaac gaattgcccg
     8941 cctgcgcgct tttctccagg aacacgatgc agacggcgtc attatcgtcc aacctgaaaa
     9001 cctgcgctac ttcagtgcct ttacgggtgg tgaaggggca ctggtcatcg gcctggacca
     9061 ggccgtctta tggactgatt cccgctatac ggaacaagct gccaaccagt ccggggcatg
     9121 gtacgatatc aagaaccacc acaatgcctt atcggcctcc atccgttcgt cactgagcga
     9181 catggaaatc ggcgtggcag cgtatgaaga aaatttcctg acccatttca tgtacgaaaa
     9241 tatttccgac ggagccttat gcggcttcct gccctgcgat ttgaccagcc tgcgggcggt
     9301 gaaggaaccg tatgagctga aagcgacgcg caaggccagc aacatcgctg accgggcctt
     9361 tgctgattta ttgccgtaca tcaagcccgg tgtcaccgaa aaggaactgg ctgcccgcct
     9421 ggaaagcaat atgctgctct taggctcgga agaaaagtcc tttacgacta tcgtcgcatc
     9481 cggcaagcgc tcggccatgc cccacggtac ggccagcccg aaagtcgtcg aagccggcga
     9541 tttcgtcacc tttgatttcg gcgccgtctg ggaaggctac cattcggata tcacccggac
     9601 tgtcgtcgtc ggcaaggcgt cgcaggccca gaaggatttc tacaatctca tcctggaggg
     9661 tcagaagctg ggcgtatcgc tcatcaaggc cggcgtttcc cgccgcgaag tcgattttgc
     9721 cgtgcgcaac tacttcgccc gctatcacgt cagccagtat ttcactcatt ccctgggcca
     9781 cggtacgggc ctggagatcc acgaacaacc agtcctgtcg ccgcgttcga caggcgtact
     9841 cgaagagaat atgatagtca cggtagaacc gggactgtac atagaaggca agtttggcgt
     9901 ccgcattgag gattccgttg ccgttacagc caacggctgt gaaattttaa ccaaaacacc
     9961 caaagatttg atggagctgt tataggagga gtttacacat gattacaagt aatgatttca
    10021 gaccaggcgt aactatcgaa atcgacggcc aggtatggca ggtcgttgaa ttccagcatg
    10081 taaaaccggg taaaggcgct gctttcgtcc gcgctaagat caagaacctc gaaacgggcg
    10141 ctgttgttga acgtacctgg aacgctggcg aaaaagtaca ggacggccac gtcgaccgcc
    10201 gccagatgca gtacctctat gaaaacgaag gcatgtactg tttcatggat aacgaaacat
    10261 acgaacagat cgaattgaac aaagaaaacc tcggcgacgc tgtccatttc ctcaaagaag
    10321 aaatgaacgt ttccgtcatg atgttcaaag gcaaagtcat cggcatcgat ctcccggctg
    10381 ctgtcgaatt gaaagtcgtc gaaaccgatc ccggtgtccg cggtgataca gctacaggcg
    10441 gcagcaaacc ggctaaattg gaaacgggct atgtcgtcaa agtaccgctc tttatcaatg
    10501 aaggcgaagt tctccagatc gacacgagaa ccggccagta tatcggcaga gcataaggtt
    10561 aacgcaagtc gttagccgcc agccgctagc atagggtatc aaaggggctt cgcacgtgcg
    10621 acagcccctt ttgtggacta aaggggtgat tcccatggaa acgactgaag taaatgaatg
    10681 ggggagtatc catatttccc agcgcgtcat tgcctctatt gcggctctga cggcggccca
    10741 atcggcctat gtcgctgatt tggggacgaa tatcgccgaa gtggcagcgg aaaagctggg
    10801 gcgtaccctt ccgggccgcg gcgtcgacgt ctctatcgac ggccggcgca tcgaattgac
    10861 ggtccgcctg gtcgtccgct atggctgccg tattcccgac gtagccctgg aagtgcagaa
    10921 gtccgtcaag gatgccgtcg aaaaggcgac ggcctgcacg gttacggcgg tccacatcat
    10981 tgtacaacag ttagtgtttg gagaggagga ccgccatgga tgaggaacag gattttgcct
    11041 atgccgtcag tgccaaggcc ctgggccata tcgccgagac ggccggccgc caagcctctg
    11101 gcgtcgtcgc tatacgcagc cgcagcgcgg ccgtgacgga cggcgtcgtc catctccatc
    11161 tggccgtccg cgcccgttat ggcagcgacc tgcacggcct ggccctggat gtccagcagc
    11221 gggcggctga ggccatcgac gccatggtcg aaccagaggg ccttgatatt tccgttacca
    11281 ttgaagacct ggcggtactg ccggctgaat aaagttatta caggaggaaa ctcgtgagca
    11341 gacataaagc gcgccagcag gcgctcgaac ttttatattc ccgtgaattc cacggagaaa
    11401 atgatatcca gtacagcgaa caggaaatcc tggaaaatga ttacgtcacg accgacgcct
    11461 tgaccgaaga cggcccggaa ctgagtgatc aggaacgcaa tgactattgc acgtatctcg
    11521 tcgaaacgac gacggaacac cgggacgaac tggatacgat catccgctcc tttgccaagg
    11581 gctgggacat cgaacggatg aaccgcacgg accgcaatat actgcgcctg gccttgtgcg
    11641 aactcgtata ccccaaggaa agcctggcgc cgtccatcgt cctcaatgaa gcggtcatcc
    11701 tggccaagga attcagcggc gacaagtcgg cgcggttcgt caacggcatt ttgggcgctt
    11761 atgtaaggtc ccaggcctga tggacgtctt tctcggcatc gataccagct gttatacgac
    11821 gtcgctgtgc ctcgtcgacg cggcttatga cgtcgtcgcc gacgaacgca tcatcctgag
    11881 cgtcgccaag ggcggccgcg gcctgtccca gtccaacatg gtctatcagc atacgcgcaa
    11941 cctgccggtc ctctttgaac ggctggctgg cgtcctggcc agtgcccata tccgggccat
    12001 tgccgtgact gaccagcccc ggcgccgcgg tgattcgtac atgcccgctt tcctggccgg
    12061 cctcggttat ggccgggccc tggccgccgt tctgggcgtg cccttgtacc acctgagtca
    12121 tcaggaaaac catctcctcg ccgtcctgcg cagcatgggc tatattggca gcgagccgtt
    12181 ctacggcatc cacctgtccg gcgggacgac ggacctgctg cgggccgtgc ccgatgaaaa
    12241 aggcctcgat atcacccgta tcggcggcac cagcgacatc agtgccggcc agttcatcga
    12301 ccgcatgggc gtcgccctgg acctgccctt tccggctggc gttcatgtcg acaagctggc
    12361 tcagacggta aatccgcccg tcaagggagc tcatgtcttt tgccgcgatg gccaggtcag
    12421 cttttccggg cccgaatcgc agggccagcg gcgcatcgcc gccggtgggg aagacccggc
    12481 ccgcctttgc agctggacgc tgtcgacggt ctggcgcggc ttggagagcc tcctcgatta
    12541 cgccgctgcc gatggcatga aacatctcgt cgccgctggc ggcgtcatgt ccaatggcta
    12601 cctgcgccgg cagatgagcg attattgccg ccgccatcag atagacctga atctggcagc
    12661 cgacggcttc agtgccgaca acgcgtcggg cgccgccttc tgggctgccg tacaggaagg
    12721 gaagaaatca tgaaatatgc atcggttacg gacgtcgtcc gccgcatcaa agatgatatc
    12781 cacggcgatt accgcttgca gagcatcgct atggaaggca acatcatcgg cctcaagcgg
    12841 gcgtcgaacg gccattatta tatgaatatc cgcgatgatc agtgctccat ccgggccatc
    12901 gtcttccgca gccgcgtgac ggcagcggtc cgggccgtcc gcgaagggga ccatgtcgtc
    12961 gtcatcggcg ccgtcaacgt ctatgaaaaa ggcggcgccc tttcctttat cattgaacag
    13021 ctcttttccc aggggacagg gtctctgcag gcccagtacg aacgcatcaa gaatgaactg
    13081 gcggctcagg gctatttcga cagcagccac aagaaggagc tgccgcgctt cccctggcgc
    13141 atcggcgtcc tgacgtcgaa gaccggcgcc gtgctccacg acatctataa gattgccggc
    13201 gagcgcaatc cttatgccga aatcatcctg catcccatcc ccgtccaggg cgacggcgtt
    13261 gatacggcca tcgccaaggc catcggtgag atgggccggc gcaaggacct ggacgtcctc
    13321 atcgtcggcc gcggcggtgg ctctatggaa gacctgtggt gcttcaaccg cccgactgtg
    13381 gtcaaggcca tttaccatgc cagcgtaccg gttattacgg ccatcggcca tgaaacggat
    13441 acgaccctgg ccgattatgc cgctgacgtg cgggcggcga cgccgaccca cgcggccgag
    13501 atggctttct ttgacgtcag cgaagcggaa atggacctgg ccgcactggc ggaccgggct
    13561 tacgaacggg tcatggcctg catcgaagac cggcagcgct gggtcgaaca ggcggcgggc
    13621 cggctgaacc tgcagcgcta cgatgccttc ctggccatgc gcgaagcacg cctggcaggc
    13681 ctgatggaac aggccgaccg ccagctgcgc ctccatctgg aacagaagca ggggaagctg
    13741 aataccctga cggcttccct gcaggccctg aacccggcag ccctcgtgcg ccgcggctac
    13801 ggccagctca tgcagcatga caagatcctt acagatatcc acagcgtgtc gaaagaggcc
    13861 ccgctgacga tacaagtcgt cgacgggacg attacgacgg ctgttaaaga ggtggacata
    13921 tatggcaaga acgactgata aattgaagcg ttttgaaagc aattatgaaa aattggaaca
    13981 gctcgtacag gaactggacg caccggacct gacgctgaag cagtccctcg atttatatga
    14041 aaaggccatc aaattatccc agtcctgcga aggcgccctg gaatatgccc gccagaaggc
    14101 ccaggccctg gccgatgtcc gggccgataa ggacagcagc gacgaggcgc cggctgaagg
    14161 ggtgttggac ttatgacctt ccaagactat ctggcaggac agcgtaagcg cgtcgacgat
    14221 tacctgtccc acgtactgac gacggaccag ccgtgttttt ccaggctcta tgaagccatg
    14281 aattacagcc tcctggccgg aggcaagcgc atccgcccgg tccttctgct ggcgacggtc
    14341 gaagccctgg aacaggatgc ctctccctat acgggactgg cctgcgctct ggaatgtatc
    14401 catacgtatt ccttgatcca cgacgacctg ccctgtatgg atgacgacga cctgcgccgg
    14461 ggcaagccga cgaaccacgt cgtctacggt gccggcctgg ctaccctggc cggtgacggc
    14521 ctgctgacct ttgccttcga gctcatggcc agccagaaag gcattgcacc ggacaaactg
    14581 aaccgctgca tcgccgtcat cgccagagcg gccggaccgg ccggcatggt cggcggccag
    14641 gccttcgacc tggcttcgga cggcgacatg gccatcggcc gggaaggcat ggaactcctg
    14701 caccgttcca agacgggcgt catcttcaag gcggccatcg acatggcagc catcgtcgcc
    14761 gatgcgtcgc agcagcagcg gcaggccctg gaagcctatg cgtcgtacat gggcctgacc
    14821 ttccagatca ccgacgatat cctcgatgtc gtcggcgatg aagctttgct gggcaaaccc
    14881 gtcggcagtg acgcccgcaa cgacaaggcc acgtatgtga ccatcttttc cctggacgaa
    14941 gcccggcgca tggcccgcga agcggccggc aaggccgtcc aggccctgga accgttaggg
    15001 ccgcaggcct ggttcctgaa agaactggtc gaacatctgc tggtccgcgt tcattaatgt
    15061 gagaggggta ttacaggtat gcagcataaa gagagattag acgtgcttct cgtcagccgc
    15121 ggcttgtctg aaagccggga aaaggccaag gcgaccatca tggccggcct ggtcttcgtc
    15181 gacaaccagc gcgtcgataa ggcgggcacg aagctgcccg tcgacgtaga tattaccgtc
    15241 aagggcaatc ccattcccta tgtcggccgg ggcggcctga aactggaaaa ggccatggaa
    15301 acgtttccca tcgacttgac gggtaagacc atgatggata ttggcgcctc gacaggcggc
    15361 ttcacggact gcgccctcca gcgcggcgcg gccaaggtct ttgccgtcga cgtcggctat
    15421 ggccagctgg cctggaaact gcggactgat gaccgcgtcg tcaacatgga acggaccaat
    15481 atccgcaacg tcactgttgc agatatcggc gagcccgtcg atttcatctc cattgacgtc
    15541 tccttcattt ccctgctcaa gatcatgccg gccgtcgaac cgctgctcaa ggcaggcggc
    15601 acgatggtaa cgctcatcaa gccgcagttc gaagcgggcc gggaacacgt cggcaaaggc
    15661 ggcatcgtcc gcgacatcca ggtccaccgc gacgtcatcc gccacgtcac ggacggcatc
    15721 gccgactgcg gcctgtcgcc gctgggcctg accttttcgc ccatcaaagg ggctgacggc
    15781 aatattgaat atctcttgta cagccgtaaa gatgacccgt gccataacga tatcaccgac
    15841 gctgtcgtcg atgatatcgt cggtaaatcc catgaaatgt aggtgatccc catgcgtatc
    15901 ggtattttcc cgaacctcgt caaacgtgaa agtgctgcca ttgtccagca gatgatcaag
    15961 atttgtgaaa aacatggcat tgaatattac ctgccagcct acgtcagcga gagccgacag
    16021 ccggcgtatc agcgcatcgg cgctgaacac ctgcggccgc gcatgaccat ttacagcacc
    16081 atcgacgtgg ccatgatcct cggcggcgac gggacgatcc tcaagatggc caagcagttc
    16141 gctgaagccg atgtcccggt ctgcggcatc aacctgggct ccctgggctt cctctacgaa
    16201 gtcgagacca agaacctgga aaagcgcatg gaagacatcc tggccggccg ctatttcctg
    16261 gaagaacgga tgatgctcca ttcggaactg tgctatgaag acggcctggt ccagtccctg
    16321 cccgatgccc tcaacgacat cgtcatcggc cacggcaacg tgggcaagct catccgcatc
    16381 gacttgagca tcaacggcca cttcatccag cagtatcccg gcgacggcct catcgtggct
    16441 acggcgacgg gtagtacggg ctatactttc tccagcggcg gccctatcgt cgcgcccagc
    16501 gtgccctgca tcatggttac gcctatctgc ccgcatctgc tgctgaaagt gcccctggtc
    16561 ctgcgcgatg acgaccaggt gtcggtgacc gtggccaaca gccgcaacag cgtccgcgtc
    16621 tctgtcgacg gcatgatgga ccaggaactc atgcgcaaca tgaccctcca ggtccgcaag
    16681 tccgaccacg tcttgaagat catccgcttc aacaagaatt atttctatag caacttattt
    16741 acgaaaatga tgggaaatga ttgacatgta tccattccgc aatgccgtta cggtatcaca
    16801 tatgatgact gaaccctacg cccgccaggc ccatgtcctc gtcgacatga cctgcggcaa
    16861 cggccacgac acggccttcc tggcccgcac catgcctgat gacgccgtcc tctacgcctt
    16921 cgatatccag ccctgtgcca tcgagcgcac gcgccagcgc ctgcaggacg aaggcctggg
    16981 ggacaaggac gtccgctgcc gctgcgggtc ccatgaacaa ctgctggctg atgtacagtc
    17041 agacattgac attatcgtat ttaatttagg ctatttgcca tctggtgacc ataaaatcca
    17101 tacaaattgt gaaataacac taaaagctat aaaaataggc ttgaataaaa ttgcgataaa
    17161 tgggataatt atgataacag cctatcccgg tacggaggcc ggggcttgtg aagaacaggc
    17221 gctgcggtcg ttcttacaga ccatacccca gcaggacttc catatttcgt cctggcaacc
    17281 cgtcaatgag gtgcattgtc cacccgtatt atatattgta cagaaaagag ggaaacgtca
    17341 ttatgaagag attccgttac accaagatta aagaaattgt ccagtcccgt cctatcgaaa
    17401 cgcaggaaga attagccaag gccttgcagg aagaaggcat cgaagtcaca caggctacgg
    17461 tttcccgcga tatcaaagag ctcatgctca tcaaggtccc gaccagtgat ggccattacc
    17521 gctacgccct gtcgccggaa cagaacatgc tcatgtccaa gaaccgcatg gcccgcctgt
    17581 tccaggactc catcgtccgc gtcgattcgg cgtataacca gatcatcgtc cataccctgc
    17641 cgggttcggc caatcccatt gccgccgcca tcgaccatgc ccgctgggaa aatgtcatcg
    17701 gcaccctggc cggcgacgac acggtcctcc tcatcgtcaa ggacacggaa acggttccga
    17761 aactggtcaa acttatcgta tcgctgatga aggaatgagg cccttatgct gcagtccctg
    17821 cacatccaca attttgctct gattgaagac ctgcacctta cttttggcga cggggtgacg
    17881 atttttaccg gcgaaaccgg cgccggcaaa tcgattctcc tcgacgccat cggtatgctg
    17941 gcaggcaaac gcgcatcggc atcgttcgtc cgtcacggga cggactcatt ccttgtcgaa
    18001 ggcgcgttct ttttttctcc cctgccagaa gggctggaac agctgctgac cgataatcac
    18061 atcgaagtcg atgaaggcca gctcatcatt tcccgccagt tccagcgcag cggccgcggg
    18121 acgatcctgg tcaacggcac gctggtgccg acgacagtcc tgcggcgcct cggcgcatac
    18181 ctcctggata tccacggcca gttcgacaac agcctgattt tcaactcgtc ttaccacgtg
    18241 gccatcctcg acggcctcac ggccgacgtc cgtcaggccc gccaggacta tgacgaacta
    18301 tatcgccagt ggcatcagac ggaaggcgaa atcaaggccc tgcgccacga tgaaagcgaa
    18361 aaggcgcgga ggctcagtat cctggccttc cagatcaagg aaatcgaagg ggccgccctg
    18421 aaagaagggg aagacgacga actggaacag aaaatcaaca aggcttccca tgccgaacac
    18481 atcaaggata acctgaaaga agccatgttt gccctggaag ggggcgaacg ccagcccggc
    18541 gtcatcgaac agatcgagac catccaccgc agcctggaaa aggcgtcggc ttacgatgaa
    18601 gctttcagtg ccctggccgg caaagtcgag actctgtcct atgaaatcga agacgtccac
    18661 gacggcctcc tgagctatgc cgattctttc gacttcgatg aacgggccct ggatgccatg
    18721 cagtcccgcc tggcgtcgat tgaaaagctt aagcgcaaat acggcttcac cgtcgccgat
    18781 attctgcaat tcctggccaa ggccaaggcc gattacgaac ggctcgacca gtccgaaagc
    18841 cacctggcca agctggaaga gaaattggcc cgtcaggccg cggctgtgcg ccagcaggcc
    18901 caggtcctgg cggccgcgcg ccgtgacgcc gacagccagt tcagccagac catgacggcg
    18961 acgctcaaca agctgggcat gctgaacagc cgtatcgcct tccacatcga accgatgaag
    19021 gacatcacag cggatggttc agccggtatc gagctctatt tctcggccaa tacgggcgaa
    19081 gatatgcagc ccctggccaa gatagcatcg ggcggcgaag tatcgcgtat tgccctggcc
    19141 ctgaagacgg ccggtccgga acggacgacg ggcaagacga tgatcttcga cgaaatcgac
    19201 gtcggcatca gcggccagac gggcctgcag gtcgccgacc acatccgcca gctctccggc
    19261 cagggccagg tcttctgcat tacccacctg ccgcagacgg cggccattgc cgaccaccat
    19321 tatttcatat ataagaaaga aatggacggc cgcacggtca cccaggtccg gggcctctcg
    19381 gaaggggaac acgtcctgga aatcgcccgc atgttcgccg gcaacgatac gaccaaggcc
    19441 agcatcgaag ccgcccgcca gatcgtaaaa caagtcaagg gcatgtaatg cccttgactt
    19501 gcgattcgac attgctttac gggttgacga atgtccgtga taggtatata gttaattata
    19561 atttcaaaaa atagaagttc tacgaaaacg aacccatagg agggatagat atgcttcgtg
    19621 tattagtcaa tggtgccgat ggccgcatgg gcagccaggt cgtcaaagct gtctttgaag
    19681 atccggaatt ggaagtcgct ggcggtatca gcatcactca tgtcggtgaa gatgtcggcg
    19741 atatcgccgg tgtcggccac ttaggcctgg ccatccgcaa tgacctgggc gccgccctgg
    19801 acgacatcaa accggatgtc gtcgtcgatt tcacgacgcc gaaagtcatc ttcaacaacg
    19861 ccaagacctg catcgaacac ggtatcaaca tggtcgtcgg tacgacgggc ctgacggcag
    19921 accagcgcga tgaactcaac gccctggcac tggaaaagaa gaccagcatc ttcattgcgc
    19981 cgaacttttc catcggtgcc gtcctgatga tgaagctctc gacagaagtc gctccgtatc
    20041 tgccggatgt cgaaatcatc gaactccatc acaacaataa actggatgcg ccgtctggta
    20101 cggccatcat gacagctgat aaaatcgaag ctgcccgcaa ggccgctggt gctaaaccgg
    20161 ctccggacaa gacccatgaa agcctcgacg gcgcccgcgg tgcctctgtc gccgggatcc
    20221 ctatccacag cgtccgcctg ccggggtacg tcgcacatca gcaagtctta tttggggggt
    20281 acggcgaact gctgacaatc cgccacgact cgatggaccg caagtccttc atgcccggcg
    20341 tcgtcttagc cgtcaaaaaa gtaagctccc gtccgggctt gacctttgga ctggaaaact
    20401 atttataaaa gaaccattag gggaggaagt ttgttatgac aaaagaaccg gtagtagcga
    20461 ttttaggtgc cacaggtgcc gtaggccagg aatttatccg cttgatcgga gaacgcaaat
    20521 tcccgtacag caagttgaag ttattagcat cgtaccgttc ggctggcaag aaattgaccg
    20581 tcaacggcca ggaatatacg gttgaagaag ccaaaccaga atctttcgac ggtgttgata
    20641 tcggcttgtt cgccggcggc tcgatcagta aggaattggg acccgaagct gctaaacggg
    20701 gctgcgtcgt catcgacaac tccagcacgt tccgtatgga cccggatgtc cccctggtcg
    20761 taccggaagt caaccctgaa gatattgcct ggcacaaggg catcattgcc aacccgaact
    20821 gctcgacgat catcatgctc atggcactca agccgattca cgacttgtcg ccgatcaaac
    20881 gcatcatcgt cagcacctat caggctgtat ccggtgccgg caaagaaggt atcgacgaac
    20941 tgcgcgatga aacggaagcg tacctcaaag gcgaagaaat gcagcccaag atcctgccca
    21001 gcaagagcct gcccaagcat tatcccatcg ccttcaacct cattccgcag atcgataaat
    21061 tcgtagaaaa agattacacc aaagaagaaa tgaagatggt caacgaaacg cacaagatgc
    21121 tccacgatga ctccatcgcc attacggcta cgacggtccg cgtcccggtc ttccgcagcc
    21181 atgccgaatc ggtctatgtc gaaacggccg atgaactgac agttgatgcc gtccgcaaag
    21241 ccatcgatgc tttcccgggt gcccaggtcc aggataatcc ggctgaaatg gaatatccta
    21301 tgcctttgtt cacgtcggaa aaatatgact gctacgtcgg ccgtatccgc aaggatcttt
    21361 acaacccgaa agccatcaac ctctgggtat cgggtgacca gatccgcaaa ggcgctgcgc
    21421 tcaatgccct gcagattgcc gaatacatgc tggcccacga catgatccac tagtacgaag
    21481 aggggagaga atcctttatg ttaacgtttc ccaatgttat tgtcgctatg attacgccgt
    21541 tccatcagga tggctccgtc aattacgaag ctgccgtcga actggctaag aaatatgctg
    21601 acgaaggatc ggccatcctc gtcggtgcta ctacaggtga aggggctgtc atgacggaag
    21661 aagaaaaact caagctcttc aaagtcacag ccgatgccct gaaaggccag accctggtca
    21721 tgggcaatgt cgggaccaac aatacggctc agaccatcgc catgattcag aaagcgaaag
    21781 atacgggcat cgatgccctg ctgtgcatcg tcccgtatta caataaaccg aaccaggaag
    21841 gctgttacgc ccactttgca gctatcgcca agtcgacgga catgcctatc ttcatttaca
    21901 acgtaccggg ccgtacaggc ggcaaaatcg aaccgcagac ggtctgccag ctggctcatg
    21961 acttcccgaa catcatcggt ctcaaagatg ccagcggcga cctggactac gccggttacg
    22021 tcgcagccaa tgcaccggaa gggttccatc tctacagcgg tgatgacaac ctgaccctgc
    22081 ctatcgtcgc tgtcggcggc gaaggcgtca tttccgtcgc tgctcacgtc atcggcaagg
    22141 aaatgaacga aatgatccag gcccacaaag acggcgacgt caagaaggca gcggccctcc
    22201 atcagaagta cctgcccttc atgaaaggct gcttctgcac ggtcagcccg gtcccggtca
    22261 aaactatgct caacatgctc ggcttcccgg ccggcccgtt ccgcctgccc ctggtagacg
    22321 ccacaccgga aataaaggcc aagtgcgaac aatacctgaa agacatcggc agattataag
    22381 tgctgataaa cggaaacctc tccgacctgt actcaagcct cccttatagg ggaggtggcc
    22441 cagtgggccg gagagatttc tataaacttg gttttatatt aaaaaaagcc ttgacatatc
    22501 cctgatttct tgttattata tatgagtcgt caggaacgac aagatatgac tcggtagctc
    22561 agctggatag agtgactgac tacgaatcag taggtctagg gttcgaatcc ctaccgggtc
    22621 accacatcaa aggactgtca cattttgtgg cagtcctttt ttctttagct aaccactaac
    22681 aactagccac tagccactta aaagggcttg tcgtcgtgcg acaagccctt ttacccactt
    22741 taggcccggc cctatccatg ctataataga gacatcgcaa attgtaaaaa ggaagtgtaa
    22801 gctatgacag tacagctcga agaattattg aaagcctttg aactgaataa tccctatctc
    22861 aagttctatt taaataaaaa tgacggccat atggccttgg tcacggaaat ccccggtgac
    22921 gacaaggcgg aaaatgaagt cacagccaat cccgatgcgt atatcaagct gccgacccag
    22981 gccgacctgg atctgccgga aatgatcaag ggctttgtcc ccatgatgaa agacccgaag
    23041 cagcgggcgg cttttcagca gtccatcgaa gcgggcaaga ccgtttccca gctggaacgg
    23101 gaattgaagg acatgggcct ggtccagttc tggtatacct tccagcgcat ggaattccgc
    23161 aaaattgcca aaaagtggtg tgaagacaac cacattgatt atgaagaata ggggagctcg
    23221 gagatgacta atatttcaga tatcggtgaa ttcggattca tcgatgctat taaagagcat
    23281 accctcttcc atccggaaaa cgtcgccgtc ggcatcggcg atgacggtgc tgtctacacg
    23341 acgacgccgg gctggcagca ggtagctgtc atcgacacga tggtccagaa cagtcatttc
    23401 atcatcggtc agacagcgac ctggcatgac gtcggcttca aggccgtagc ctcgaacctc
    23461 agcgacatcg ccgccatggg ggccgtgccg acccatatcg tcctgtcgac agccctggca
    23521 ccgcagatgg acgtcgatga cgtcattgac atgtaccgcg gcatcaagga tatttgccgc
    23581 acgtatgaag tcaatatcct cggcggcgat acggtcatgt ccaaagaagg cgtcgtcatc
    23641 actgtcgctg cctttggcga aatcgaagcc ggaaaggccc tgctgcgcag cggagcccag
    23701 gccggcgacg tcatcgccgt atcccatacg atcggcgatt cggcaggcgg cctcgacgtc
    23761 ctcctggccg gacaggacgg atatgaacat ttgaagaaag cccaccagta tccggaaccg
    23821 cagatttccc tgggacgctt actggtacag catcattgcc acagcctgaa cgacatcagc
    23881 gacggcctgg ctagtgaatc caatgaaatt gccaaggcca gcggcgtcga actggtcatc
    23941 gaccgggacc aggttccgac gagcgctgag ctgaaagcct gggctgcggc atcgggcaag
    24001 gatatttgga aattcgtctt taacggcggc gaagactatg aactggtctt taccatggcg
    24061 ccggacgatt tccaggcttt gcaggccgtc tatccggctg tgacgcctat cgggacggtc
    24121 cgccaggggc cgggccgggt cctgctccgc cacgatgggg gcgaacaggt cctgccgccg
    24181 acgggctgga cccattttta ggaggaggat atcatgagct ttgatgtagc aactcattca
    24241 gaagcggaca ccatcccttt gggcaaagcc ctggggccgg tcctcagaga cggcgatgtc
    24301 ctggccctgc gcggcgacct cggtgccggc aagacccatt tcgtccaggg catcgcccag
    24361 ggcatgggca ttgacgacgt cgtcgtcagt ccgaccttta ccatcctgaa ttattatgaa
    24421 aataccattc ctctccagca tttcgacttt tatcgcttgg aagaggaata tgaattagac
    24481 gatctgggat tcgacgacta tctcgaaagc ggcgtgaccg tcatcgaatg gtcggaaaaa
    24541 ttcccggacc gcctgcctga cgatgcggct gtcgtcacca tcgaaaagac gagtcccacg
    24601 gaccgcctgt tccattttga cttccacggc agccgttggc aagctgtaga aaatgaggta
    24661 aaaaaatatg ctcttagcca ttgaaacatc gagcctcgta tcgagtgtcg ccttgctcca
    24721 tgacgacacg ctgcgggctg aactcaccat ccaggcacgg ctgacccatt cggaacagct
    24781 catgccccat attgccgaca tgctcgacaa ggcgtcggtc aagaaaagcc agattgacgg
    24841 cgttgccgtt gccgtcggcc ccggttcctt tacgggcctg cgcatcggcc tggccacggc
    24901 caaaggcctg tcctttgcct ggaacgtgcc tatcgtcggc gtcacgacgc cggtcagcct
    24961 ggcctggaac ttcgtcggcg tctctgaccg catctgcacc ctcatcgacg cccagaaagg
    25021 caatgtctat gccggtgtct atcgctggga ccggacgacg ctgacgacgg aaaaagaaat
    25081 ctacatcgcg ccgcggacgg aagtcctgga taccctggaa agggaaggcc agcccgtcgt
    25141 cttctgcggt gacggctccc tcaaagggcg ccgggatatc gaaggccgga gccccttgtt
    25201 ccgcatggcg ccgccgacga tggtcattcc ccgtgccggc agcgtcgccc tggcagccaa
    25261 tgtccgtttt gccgccggtg actacgatga ctgcatgacc ctgacgccgt cgtacctgcg
    25321 ccgcagcgaa gctgaagtgc tctgggaaaa acgccacggc ggtgccccat gtcagtaacg
    25381 gtccgtcagg cctgtcggga ggacctgccg gatatcatcg ccatcgaaga ggcgtccttt
    25441 tccgtgccct ggtcccatca gtccctggca gcggaactgg acaatcccgt agcccgttat
    25501 tatgtcctgg aaaaggaaga cgggagcgtc gccggttatg ccgacgtctg gctcatcgcc
    25561 gatgaaggcc agctggccaa catcgccatc catccgtcag cacggggcct gggctatggc
    25621 gagattctcc tgcgaacggc catggaagcc ctcttccggg aaggctgcac gagtttcttt
    25681 ctggaagtcc gccaatccaa cgcgccggcc cagggactgt accgcaagct gggctatgaa
    25741 gcccttgctg tccgcaagga ctattattcc cagcctgtgg aagatgcgtg catcatgcat
    25801 tgtcaaaaag agaagtatca atggctcggc cattcactat agaaaaaagg atgaaatcta
    25861 tgtatacctt agctttagaa acgagttgtg acgaaacgtc ggctgctgtc ctcaaagatg
    25921 gccgccacat ctgctccaac atcatatcga cacaggtgcc gatccaccgg aaattcggcg
    25981 gcgtcgtacc ggaaatcgcg tcgcgccagc acatcgaata cgtcatgccc atcatcaagg
    26041 aagcccttga tacggctggc gtcaccttgg cagatatcga ccatatcggc gtcacttacg
    26101 gtcccggcct ggtcggggcc ctgctggtcg gcgtcgcagc ggccaaggct atcagctttg
    26161 ctgccgataa gcccctggtc ggcgtcaacc acatggaagg ccatatcttt gccaacttcc
    26221 tcagccatcc cgatttggaa ccgccgttcc tggccctcgt cgtatccggc ggccataccc
    26281 agctggtcca gatcaatggc tataattcgt tcagactcat gggccagacc cgcgacgatg
    26341 ccgccggcga agctttcgac aagatcgccc gcgtcatggg ctatccctat ccgggcggtc
    26401 cgcagatcga caaactggcc aaagaaggcg atcccgacgc catcgccttt cccaaggccc
    26461 tccatgaaaa gcacaatttc gagttcagct tcagcggcct caaatcggca gtcctcaact
    26521 acctgcacac acaggaacag cggggccagt cctataatgc cgccgacgtg gcggccagct
    26581 tccagaagac cgtcgtcgaa acgctcgtcg aaaagaccat ggatgccgcg gcctactgcg
    26641 gagccaggaa aatcgccgtc gccggcggcg tctcggccaa cagcggcctg gctgcggcca
    26701 tgagtgcggc ctgtgccgac aagggctatg ccttctaccg gccggaaccg atactctgca
    26761 ccgacaacgg cgccatgatc ggctgccggg catattatat ggccctggac ggccgctttg
    26821 ccgacttgac cctcaacgcc aagccggccc tgccgattac atcggactaa tcctgagaag
    26881 agatggggga atcttctatg gctacactcg aagcggtctg ccggacaagg agcaccttgt
    26941 cgcccgtgca gatccatatt ttacagaaca gtgaagaact cttgcagttc gccagcgact
    27001 tgtcgcaccg ggaactcttc gtctatgtgc ccgggcggga agcggggacg ctggtcctgg
    27061 ccgcgcaccg gacgccgctc ctgcagaagg ggaggcagac ggaaacgact gatcctggcg
    27121 ctgccttttc ccgttatgaa gaaccgctta attatcaggt ctttcaggcg ggaacggcca
    27181 tgagcggtga gaaagaaatc gaatacggcc gcatggcgcc catcgtggct tatcccttcg
    27241 tcgataacgg cggcgggacg attgccgtca tttcctttgt cggcacagtc gaagacaatc
    27301 gggagatttt gacggaaaca gccttcttat ctttgcaggt gcccatggat ttccattcca
    27361 tgtaccggcc cctgtccatc caggatggcg tcgtcctggt caactgcaac ggcgtcgtca
    27421 tctatgccga cgatatggcc gacagcatcc tgcacatgtg cggccagcgc ggggccatta
    27481 cgggtaccaa tatctataat acccgcctcg acctgaccgg tgccaagaaa gccctgtccc
    27541 aggccgaggg attggccgat gacgtgacct taggcgatgc tgtcgtttcc cgccgggtca
    27601 tccctattct gcagcgcggc aaagtgcgcc gggtcatttc catcctgact gagcggacgg
    27661 aactgcatcg caaggaagaa gaactcatgg tcaagacgtc ggtcatcaag gaaatccacc
    27721 accgcgtcaa gaacaacctg cagaccattg ccagcctcct gcgcatgcag atgcggcgca
    27781 cgacgagcga cgaagcgcgg gatgtcctca acgagagcct gaaccggatc ctcagcattt
    27841 ccctggtcca cgagacgttg tcccatcacg atgaagagaa catcgacatt tccgacgtcg
    27901 cccagaaact gctgggcctc ctggcgcaca gcctggtcag caaggattgc cacgtggaga
    27961 cgaagttttc cggcgacacc ctgcccatgc cgtctgatgc ggcgacatcc ctggccctgg
    28021 tcctgaatga actcatcacc aatgccatca tccacggctt tgaaggccga cgggaaggcc
    28081 tcctggccgt caccatccgc cgcgacgggc cggacggggt catcatcgtc tgtgatgacg
    28141 gcgtcggcat gtcccacgca cccgtcgata cggggcgcaa acatctgggc atggccatcg
    28201 tgcgcacgct cattgaaaaa gatctgcagg ggacgattga tttcgatgac ggcgtgcctg
    28261 aagggaccgt cgtcaccatc cgttttccgt taccagagag gggagaataa ccaatgggat
    28321 atcgcgttgt aataggggat gacgaatcga tcatccgtat ggatttatgt gaaatgctgg
    28381 aagatgccgg ccacaccgtc gtcggagaag ccgctgatgg cgtcgaggcc ctcgatttag
    28441 tgcgcaaaga aaagccggac atcgtcctcc tggacatcaa gatgccccgc ttggacggca
    28501 tccacgcggc ccgcatgatc ggccacgaag ggctggcacc ggtcctgctg ctcacggcgt
    28561 acagtcagca ggatatcgtc gacaaggcca aggactccgg tgtcctgggc tacctggtca
    28621 aaccggtcag tccggtcaat ctcttcccgg ccatcgaaat cgccatttcc cagttccgcc
    28681 accagcgcga agtggcacag cagctcgatg acatgaacga acgcatcgag acgcgcaaag
    28741 ccgtcgaccg ggccaagggc tgcctcatgg acctttatca tataccggaa cacgaagcct
    28801 accgacggct gcagcagtac agcatgaaga accggaaatc cttgaaagcc gtgtccgagg
    28861 cggtcattgc cagtgcggaa aagataaaaa gtcaaaaaaa ataaaacgtt tgatgaaaaa
    28921 gtcagggtga aagccttgac ttttttacct ttaatggcta atatatatat tgtaagttag
    28981 cactcggaag tatggagtgc taataaagag tagcaactta cttttaagga ggaatgtaaa
    29041 atgttaaaac cgttaggaga tcgcgtag
//
LOCUS       DAWABZ010000008        28587 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_473_length_28587_cov_3.453701, whole genome shotgun sequence.
ACCESSION   DAWABZ010000008 DAWABZ010000000
VERSION     DAWABZ010000008.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 28587)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 28587)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..28587
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_473_length_28587_cov_3.453701"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3504..3513
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    24736..24745
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6830 a         7548 c         7853 g         6336 t
ORIGIN      
        1 aatgaggtcg atgatgatgg cggcgatacc gacaccgaca ccgaagagga tgtagttcca
       61 gtcgaggctg gcactgaaga taccttgggc gatggtcgtc atcaaggttg cctgcggagc
      121 tgccagggcc tgggtcggat ccatgccggc acggggaagg gcaccggtga agccataggc
      181 ctgatagagc aggttcagga ccggagcgat ggcgaaggag ccgatgatcg agcccaggat
      241 gagggcgacc tgctgtttcc acggcgtagc gccgacgagg tagcctgtct tgaggtcctg
      301 caggttatcg ttggaaatag aagcgatgct gacgatgacg ctggtgatga agatagcaaa
      361 ggccgtagcg aactgcgtgc cctgcatggt ttcaaagaca ccggccgaag agccgatacc
      421 gaggacgacc agggacgata cgatgatgcc caggatgccg ataccggaaa tcgggctggc
      481 cgacgtgccg atgaggcctg ccatgtaacc gcaggcagca gcgacgaaga agcccatgag
      541 gatggcgacc aggatgccga cgacgatgaa catgagcgtt accgacatgg aaaggccggc
      601 gttgctgacg aaggtgtaga aagtgatgaa caagccgata aggatggcca ggaagaccag
      661 gccgaccgat ttcggcgaca tgtcgatgtc cgtatgatgg agtatcttct tttcttccgt
      721 atcgttggcg cgcaatgcgt tgatggacat cttgatgccg tcgacgaccg gtttggccag
      781 gcggatcaag gtccagacag cggcgatacc gatgcagccg gcaccgatga agcggacttt
      841 ctgagcccag atggccttgg cgaaagcggc ggcactctgg ccttcagccg gcgtgatgac
      901 cgacgtcaga tagggaacga agacgtacca ggccaggagc gtgccgacta agatagccat
      961 gccgctggcg atgccgatga ggtaacccgc gccgaggagg gctgtcgaaa agcccagggg
     1021 aatctgggtg acggctttgc cgaacgagag ccagtagctg aattcggaag aaacgacgtg
     1081 gaagccgttg gaacagagac tgaagagacc agccagggcc gtgccgccga cgatatcttt
     1141 catgcctgtt tcttttttgc ttgtgtcttc gccgttggct tcttccttgc ggatttcact
     1201 gccgactttc aggatttcag cagcagcccg gccttccgga tacggcaggt cgctgttgac
     1261 gaccatagcc cggcgcaggg ggatagtgaa caagaccccg agagagccgc cgcaggcgca
     1321 gagcagcagg gtctgccaga acgggaagcc ctgccagtac cccagcatga gcaagcccgg
     1381 caggatgaag atgattgcag ataaagtgcc ggcagcagat gcctgcgtct gtaccatgtt
     1441 attttccagg atgttggaat gtttgaacat gcgcaggatt gccatggaga tgactgcagc
     1501 cgggatggac gacgagaacg tcaggccgac ttttaagccc aggtatacgt tggaagccgt
     1561 aaagataacg gtgatcaggg cgccgaggat cataccgcgg gccgtcaatt ccggcatact
     1621 tgggtagttc acagtcagat ttgattgtgg tttcatagaa aaacctccca aataaaaatt
     1681 aagcagtact aaaacgtaca acttcactga cgcttatatt atactcaatt tttttgcatt
     1741 cgtcatatat caatttgtac actgtacgcg gaaaaacagc tgtataatta ctttttcgca
     1801 agcaaaaata tacacatgcg gatagtagcc cgatgcgcct cgtccgtggt ataataaata
     1861 aacgattaag caagtcaatc gcagagactt tttgaaggga gcgtttgtca tatgaacgat
     1921 catgaaatca ttgaaggtgt catccacgaa tttgcaggac tggccaagca tccgcgtccg
     1981 tcccaccatg aaaaaggcgt cagcgattat atcgtcagcc gtctccatga actgggcgtt
     2041 gccgatgtaa cacaggacga agtatataat gtcatcgcca atgttccggc cagcaagggc
     2101 tgcgaagatt ggccgcttac ggtcctccag ggccatatgg atatggtctg cgtcgccaag
     2161 cccggcgtcg atttcgaccc cttgaccagt gaaatcaaac tggtccgcga aggcaacatc
     2221 ctccacgccg atgggaccag cctcggtgcc gatgacggca ttgccgtcgc cttggcgctg
     2281 tatctcatcc agcaggatat ccagcacggc ccgctgcgcc tcatctttac ggtcgatgaa
     2341 gaaacgggca tgaccggggc tacccatctg gatccgaaat acgtccagga tgtgcagtac
     2401 atcatcaact gtgactctga atacatggat gtcctctgtg tcggctcggc cggcagcgtc
     2461 catacgcatt tcagccggcc catccactgg caggctgctg aaggtaaaca ggcgtttact
     2521 atcacagcca aagactttgc cggcggccat tccggtgaaa ccatcaacga cggcaagagc
     2581 aacgccatca aggccctgag cctggccctc cgccgcattg cccaggccgg cgtatcctat
     2641 gtcctggcat ctatcacggg tggcgtagct gccaatgcta tcccgtcgga agcctcggct
     2701 gtcatcgttg tcgacgatgt aaacgcaggt gaaacggtga agaaagccgt cggcgaagaa
     2761 caggccgaaa tcgcggaagt gtatggtgaa gtcgaaaaga acgcccactt cctcgtcgaa
     2821 agtgccgacg taccggccca gaccttctcg gctgacgata cgaagaatct ggtcagcctg
     2881 ctgaacatcc ttcactgcgg cgtcttcgcc atgaaccagc tgctgccgaa gctgccggac
     2941 ctgtcggcca acatcggtac gatccggact gaaggcgacc atgtcgccat ccagtatttc
     3001 ccccgcgctt cggccgatgc ccgcctgcgc gaattgatgg ctatcctccc cgtttacgca
     3061 gagttgacag gctgtgacat cgaactggct tcgccggaac cggcctggac ggaaaatccg
     3121 cacagtaaac tcgttcccct cgtcgctgac gtttacaagg aagaagccgg caaagaagcc
     3181 cgcatcgaag ccatgcacgg cggcctggaa acagggtact tcttctccat gaacccgaaa
     3241 ctggacatca tctccgtcgg cccgacgacg catgacatcc acagcgccga tgaatcggtc
     3301 gaactcgata cggtagccct cctgacccgc atcgtcgcca agacattggg aaaattaaat
     3361 aattagtggc tagtggatag tgattcgtgg ctcgtagggg cttgcatgtg gcaagcccgt
     3421 tcctggatat atgtgatttc ccagtcaggt gtcatgggcg tcatataaat acccagcggg
     3481 tcgcccacgt ggcgacccct acannnnnnn nnncctacag taaaacttaa caaaaagagg
     3541 ctgtcttcat gcgacagcct cttttgttgt atttgcaacg gttttatttg gatatttttg
     3601 ctatagtata agtgtacgaa aaggggggag tatgatggaa aaaacgcata ttgaccgact
     3661 ggttacaccc gattatttta accgcaagga atgtgaaggc tgcggctgtt gcatcagtat
     3721 ctgcccgtat gaagctttgt cgatgcagac ctgcgccgac ggcatccggc ggccccgcgt
     3781 cgatatgact aaatgtctgc gctgcaaccg ctgtatggat gcttgtgaaa tttacaagtg
     3841 ttactatatt cctcaaaaac aatatgaaac tgccaggagg gctgcactat gaaatactcg
     3901 ctgaatccac acatctttgt ctttggcaag accatcaaga agccgaagat ttccatttct
     3961 tttttatctt acctgcttcg ggggaaacag aatatcctca tcgacacggt tcccgataaa
     4021 gcagccgatg cctatatcca ggacatcgaa agtgtcctgc ctgtcagcca gatcgatgcc
     4081 ctgattctca accattccga agaagaccat agcggagccc tgcaggccgt cctggaccgt
     4141 catccccagc tgcccatcta ctgcacgccg gccacgaagg aacggctcca gccgacgtat
     4201 ccggaagctg atttccgcgt cgtccagcat ggcgaaagcc tgacgttagg cgattatacc
     4261 ttccagttca tccatacgcc ggggctccac tggccggata atatggtcac ctggctggct
     4321 tcggaaaaaa tcctcttctc caatgacctc tttggccagt atttagccga agaaccgccg
     4381 gtcgatgccg gttattccaa ggaagccatc ctcaaaggca ccgaacccta ttttgaacgg
     4441 gtctttgccc ctgcctctga aagcgataaa gccatcatcg ccgatatcgc cgctatgcct
     4501 atccagacag cagcaaccgg ccatggcctc atcttgcaga gccaatggcc ggctgtccgg
     4561 gatttctatg tatcacacgt gatcaaataa atatttcgta tcgtaactga ggtccatgta
     4621 ctgcgcaatc gtttcgtcgt cgaatccgta ttcttccgac ttatgggtca aattacccca
     4681 tttgatctgg agcttcttct tggaattcaa gggttcttcg tattcgatgc gcagttcttg
     4741 tgcttcttgg gagtggtcct ggaccttgac aaacaggcct ttgtcgaaag tcagctcgta
     4801 gacttttcgg aaagcatggg gaagctggaa accgaaggga acgaagtatt tatcgatgaa
     4861 gctgttggcg atgaggatgg acccgctgta atccatgacg tggttgagtt catatttcag
     4921 gtcccctgcc ggcgaatgga aggaaacggg atggatgccg tcgatggcag gcggttccat
     4981 gccgttgtta ttggtcagca atttatggag ggtcagcatc ttttcatcgt tgaagccgaa
     5041 gatcgccgta tatccgcgca ggcaggcttc attgtacatg atgggatgga ggccgaattc
     5101 ctcgatgtta aagaagtcgt ccacctcttc gatggcgacg atactgaatt tatacgtggc
     5161 aaaatcgaaa ccgtcaaaaa attgggcgcg catgatgatt ctccttattc gacgaagaca
     5221 ggtttgttat tttcaaaacg ggcgatgcgg gccagagatt cgacgcggaa gcctttatct
     5281 tccaacgtcc tgcggccgct ctggaaagtc ttttcgacga cgatgccgat accggcgaca
     5341 gtacagcctg cctgggcgac gatgtccgtc aggccgacgg cagcagctcc actggccagg
     5401 aagtcatcga tgatgagaac cgattcgccg gcgtggagat aggctttgct gatcgttgcc
     5461 gtatattctt cttctttcgt atacgagtag atcttggccg tatactgttc gcccttggtg
     5521 agcagggatt tcttcttgcg ggcaaagacg ataggcaggt tgcccatggc cagggaagcc
     5581 gcataggcta cggcgatgcc cgaagcctcg atggtgacga tgcggtcgat gtgtacgtct
     5641 ttgaaacggc gggcgaactc ctgaccgacg tgattgatga ggttcgtatc gatttgctgg
     5701 ttcaaaaagt tatcaatctt caaaatccgg ttgtcgatga ccaggccatc ctgtaaaatt
     5761 cgctgtttca attcttccat tacttccatg tcctccctgt gatgaaaaat ggtaaacatt
     5821 gtctactata atagtactag tctaatacga ttttataggc aagtcaataa aattctaagt
     5881 ttacttatgg ttttaaaaca ggtatactaa agttgtctaa tgataagtta tctatctttg
     5941 agggaggtca tgacatgcag tctatccgac gtttgtatgt tgccaagaac ggtgtttttg
     6001 ctgacgaagc aaaacgattg ctcactgatt tacaggaaaa cctgttgatt aaagggttga
     6061 ccgatattca catttaccac cgctatgacg tatccgggct cgatgatgcg gcttttgaag
     6121 cggctaaaaa tatgattttc tccgaaccgc ctgtcgatgc cgtctatgac gaattgccgg
     6181 ttggcaagga cgacacggtc ctggccgtcg aatatctgcc gggccagtac gatcagcgcg
     6241 ccgattcggc catgcagtgc ctgcagatgc tgaccatgaa gaacgacagc ctcgtccgca
     6301 cggcacaggt cctcgttttg tccggcagct tatccgctga tgacgtcgct gccatcaaac
     6361 attattgcat caaccccgtc gaagcccgcg aagcctcgct ggaccccgtt tcgacgctgg
     6421 aaatgccctg ggaaaaaccg gctgacgtcc ccgtcatcga cggcttcgat gccatggatg
     6481 acgccgcttt gaaagaactg tcggcacaca tgggcctggc catgagcttc gacgatttga
     6541 agttcgtcca gacctacttc cgggatgaag aaaaacggaa tccgacagtg acggaaatcc
     6601 gcgtcatcga cacctattgg tcggaccact gccgtcatac gacgttcatg accgaactca
     6661 cggatgtttc ttttgaaaac gggacgttta cggcgcccat ccagcgcgct tatgaatcat
     6721 ataagacgac gcgggaaaac ttgaaccgta agaaaccaca gaccctcatg gatatggcga
     6781 ctattgccgt caaagaattg aaagctgccg gtaaactcga taaccttgac gaatcggaag
     6841 aaatcaacgc ctgcacgatc atcatccctg tcgatgtcga tggcgtcgaa gaagaatggc
     6901 tcctcctctt caagaatgaa acccataacc acccgacgga aatcgaaccg ttcggcggcg
     6961 cagctacctg cctgggcggc tgtatccgcg accccttgag cggccgttcc tatgtctatc
     7021 aggccatgcg cgtcaccggt gctggcgacc cgcgccagtc ggtcaaggat acgctcgtcg
     7081 gcaaactgcc ccagcgtaag ctgacgacag gcgctgccaa gggctacagc tcttatggca
     7141 accagatcgg cctggctacg ggcgaagtca aagaatatta caaccccggc ttcattgcca
     7201 agcgcatgga aatcggtgcc gtcctcggcg cagctccgcg caataacgtc gttcgcgaag
     7261 aaccgctccc tggcgacatc atcattctcc tcggcggcaa gaccggccgc gacggctgcg
     7321 gcggtgctac gggctcgtcc aagaaacata ccgtcgaatc cctggaaacg tgcggcgccg
     7381 aagtccagaa gggcaatgcc ctgacggaac gcaagatcca gcgcctcttc cgccgccatg
     7441 aagtcacggt actcatcaaa cgctgcaacg acttcggtgc cggcggtgtc tccgtcgcta
     7501 tcggcgaatt gacagacggc ctggacatca acctcgacaa agtcccgaag aaatacgaag
     7561 gcctggacgg tacggaactg gctatttccg aatcgcagga acgtatggct gtcgtcgtcg
     7621 ctccggaaca cgtcgatgaa ttcatgaaat atgccgccga agaaaacctg gaagcgacga
     7681 tcgtcgctgt cgctacggat acaaaacgcc tggtcatgca ctggcgcgac cagaacgtcg
     7741 tcaatattac ccgcgctttc ctcgatacca acggcgtcac ccagcatcgc aaagccgtcg
     7801 tcgtcgctcc ggaagacaaa gatttcttca aggctccgga agtcaaagac gtcgccaaga
     7861 cctggctcga taccatgggt accctgaata tcgcctctga acagggcctg gccgaacgct
     7921 tcgacagcac catcggcgcc cgcacggtcc tcatgccctt tggcggcaaa taccagaaga
     7981 cgccggtcga aggcatggtt gccaagattc cggtcgaaca cggcaatacg acgacggcca
     8041 gcatcttcac ccatggctat gatccggacc tggctatctg gagccccttc cacggtgccc
     8101 tctacgccgt catccagtcc attgccaaac tggtagccct cggcggcgac cgcaaaaaag
     8161 tctatctgac catgcaggaa ttcttccaga gcctgggaac caatgaaaag gcctggggcc
     8221 agccggtcag cgccctcctc ggtgctttcg tagcccagca ggtcatggaa gtggccgcta
     8281 tcggcgggaa agacagcatg agcggtacct ttgaaaacct caccgttccg ccgacgctcg
     8341 tttcctttgc cattgcaccg gaacacacga accatatcat ctcgccggaa ttcaaagccg
     8401 ctgacgacgc agtcctcctc ttcgacctgc cccgtgacgc tgaagaaatg ccggacttcg
     8461 atacttttaa agcccactgc gatttcctcc atcaggctgt cctcgatggt aaaatcaaag
     8521 ccatgcatgc cgtcggtcag ggcggtatcg ccgctgccgt cgcccagatg gcctttggca
     8581 atggcattgg cttcgccgtt gatgcatcct ttgctacgca ggaactgttc aacctccgct
     8641 atggctcgat cctcgtcgaa accgacgctg ctacggctgc tgaatgggtc aaacgggctc
     8701 acgtttccct cgtcggcaag acgacggctg ctgctgatgt gaccgtcgat ggcgtgacga
     8761 tttctctgaa agacctccag gatgcctggg aaaaaccgct gagccacgtc ttccccatca
     8821 agagcgaaag cggtacaggt gatgccgaac tgccgctgtt cacgacgtat ggtccgcgcc
     8881 gcagcgaagc ctttggtacg ccgcgggtct tcattcccgt ctgcccgggc acgaactgcg
     8941 aatacgactc ggccgctgcc tttgaacggg ctggcgctac gacggatgtc atggtactgc
     9001 gcaacaacac gccgcaggat ctggcagaat ctatcgatga aatggctaaa cgtattgcca
     9061 aagcccagat catcatgttc cctggcggct tcagtgccgg tgacgaaccg gaaggttcag
     9121 gcaagttcat tgccaccttg ttccgcaacc cggtcctgtc ggaagccctg gaaaacctgc
     9181 tctatcagcg cgatggcctg gctctcggca tctgcaacgg cttccaggcc ctgattaaac
     9241 tcggtcttct gccttacggt catgtccagc cgttgaaagc tgacagcccg acgctgacat
     9301 acaataccat tggccgccat ctgtcgcgca tggtcgaaac caaggtcgtt tccgtcatga
     9361 gcccgtggtt cagcagctgc aaagccggtg acgtccacac cgtcgccatc tcccatggcg
     9421 aaggccgttt tgtcgccaca ccggaacaga ttcgcgaact ggctgccaaa ggccagattg
     9481 ccacacagta cgtcgacctt tccggcaaag cgacttatga cagcgaatac aacccgaacc
     9541 agtcggtcct ggcagtcgaa ggcattacca gccccgatgg ccgtgtcctg ggcaagatgg
     9601 cgcacaccga acgcttcagc gccgaagacg tcttccagaa catcgctggc aacaaactcc
     9661 agctgatctt caaatcgggc gtagaatatt ttaaataaaa gatagttttt agtttgcagt
     9721 ttatagtttg tagcggatgg caaaaatttg aaaccatctt ctacaaacta caaactccgt
     9781 actgcaaact agagggaaaa aggggctgcg catggtggta aaaaccttcg tgcgacagcc
     9841 ccttgcattt ccccatcacc tatgatataa ttaacaagct tgaactgtgt caagctccta
     9901 acccttctgc gggaagggtc atatgtccaa aagaggaggt gatttcgtga acaagtacga
     9961 agtcatgttt attgcaaaac cgctcgaaga agcagaagta gatccgatcg ttgaattggt
    10021 atccaacctt ctcaccaaga acggctgcac tatcgaaaaa gtagatcgct ggggtaaacg
    10081 tcatctcgca taccctgtta aaaaacaggc tgatggctac tacgttttga tcaactttga
    10141 aggcgaacct gacgtcatca aagaaatcga ccgtgtcatc aagatccacg atactattct
    10201 gaaacacttg atcgtcaaga tcgacgaata accggaggcg caaaggtatg aactcagtcc
    10261 agttattagg gaatttagct cgtgacccgg aaatccgttt tacgaagacc ggccgggcag
    10321 tcgctatgtt taccgttgct tgtacgcggt cgtatattcc ggccggcagt tcagaaccgc
    10381 gggaattaac tgattttatt ccttgtgttg cttggggtaa tttggcggaa aactgtggca
    10441 ataacttgtc caaaggcagc cgtgtattcg tccagggccg tatctccgtt cgttcttacg
    10501 aaacgcagga tggtcagaaa cgctatcgca cggaagttgt atgcgacttc gttgcccaga
    10561 caatgggatc agaacaccag caggctgctc cggcaccgca tgctagtgca ccgcagccca
    10621 aaccggctgc accggggacc ggctttgaca gcatgggttc cagcacagac gaagaaatcc
    10681 cattctaaaa ggtcagacac accccttcta attcttgaag gaggaaacta tacatgagaa
    10741 gagatcgctc gagaaaaccg agaagaaaag tatgcagctt ctgcgtagat catgctgaac
    10801 agatcgatta caaagacgtt gcaaaacttc gccgtttcac aacagaacgc ggtaaaatct
    10861 tgccgcgccg catttccggc gtttgcgcta aacatcagcg taaactgact gttgccatca
    10921 aacgcgcacg tgcaatcgca ctcttgccgt atacggctga ataataaaaa aagacttgtc
    10981 gctttgcggc aagtcttttt tttgtagggg cgacacgtgg ggcgcccgca tcggaatatg
    11041 tacgattttc ctgtcagggt gctaggcgaa tcatacaaat gcaacgggcc gccctttggg
    11101 acggccccta caacggctaa cggctaactc catctacata gcgggcgatg atgttgcggt
    11161 cgtcgccgtc gtagatgaac tgctggagtt tttcaaaagg cgtccgttcc aggccttcga
    11221 tgtcgtccat gtggatgacc agggcgtcaa aggcgtagcc tttttcaaag ctgccggtct
    11281 ggccgtagaa gcggcccggt cctttggtgg ccaaatacaa gccttcaccg atggtgaggg
    11341 ctttttctct atcgtgcttc agggaataga gtttcgatat ttcaatggtc aaggcaatct
    11401 gcttgttcat ggccggtgta tggccggcag cgacgtcgct ggcaatcgtg caggtcaggc
    11461 cgttttccag gttgcgccgc aagggcatga tgccactggt caggttggca ttggactggg
    11521 cgcagtgtgc taagtaaacg cctttatcgc gaagtaaacg tttttcttcg tctgataaat
    11581 agatggcatg ggccatgata gtcttttcgg gccgcaagag tccaaaggta tcatagacgg
    11641 ccgtataaga cggcaagtcg ggatggagct ctttgaccca ggcgacttca ttccggtttt
    11701 ccgacagatg ggattggacg ggcaagtcgt aggtttcaca gaggcgaccc aggccggtca
    11761 tcagtttttc tgtcgtcgac gggacgaagc gcggcgtcgc gatgaagcgg accttgtgca
    11821 atgcttcgcg gcagcggacg gccagctctt ccgtatcggc cagggagcgg tccgtatctt
    11881 ccaggaggta gtccggggcg ttgcggtcca tgtttacttt gccgatatag gctttgaaac
    11941 ccatggtttc ggccagttcc atgagctgcc acgtcgattc tttatgaatc gtcgcaaagg
    12001 cggaaaaaca tagggtccct tcggcctgga ggcgtttcag gaaggccgta tagacgcgac
    12061 gggcaaagac gaggtcggcg aatttccctt cggccggaaa ggtgtaatgt tccagccagg
    12121 gaaggagttc ttcgtcaaag ccgaggccgc ggtttatgta ttgcggggcg tggatatgca
    12181 ggtcgacgaa ggccggaatg atgatatcct ggttgtaatc gacgactgtt tccgggcctg
    12241 tcgtttccgg caggtccgtc atgaagcccg ttacgacgcc gttttcgata tgggcatatt
    12301 gattttcacg gatcgtcagt tcatggcgtg acggcgtgaa gagaaaattt cctttaatga
    12361 acatgtaaac gctccttgtc tatcgtttac gcaggtacgt aaacggataa gaatgatggc
    12421 aggacttcga tgtccaaggg gaaagccggc ccttcgtcgc cgtccatgtt ggtcgagaga
    12481 tggctgccat ccagcagttc gatgcggacg cggtccatgg tcttcaaggt catcagttcc
    12541 gacgtcagcg gctggccggc caggaattcc gggatgacct tgatgaggtc cagataattg
    12601 aagtccttga agatgcccag ccagattttt ccgtcgtcga cgcgggcctg gggcgccagg
    12661 ttgcggaaac tgccgacgga attggtcagc atgacgacga tgaggggcga gcggtggact
    12721 gtcgagccct gggccgtcgt gatcttgaac agcgacgtct tatgggcctg catggccttg
    12781 atgccggtca ggaaataggc cagcgggccg aggcgcgtct tctgttcgat ggatacgtgg
    12841 ccgacggctt cgggcatgat gccggcggca atggtattga cgaagtagcg gtcgttggcc
    12901 ttggcgatgt cgactttgac cgtcttgcag tgctgcagca tggcgatggc agcttccgga
    12961 tggaggggga tgttcagggc ccgtgccaga tcgttgatgg ttcccagggg gatgaagccg
    13021 aagtggatgg cactgttggc ctgtgccaag ccgttgatgg tttcattcag cgtcccgtcg
    13081 ccgcccatgg aaaataccga atcgaagccg gtcagggctg cttccttggc ccattccgtc
    13141 gcgtcgccgg ctttctgcgt ttcccgcaat tcgacgtcgt cgaacatcgt ttccagttgt
    13201 tgtcttaaat tatccttgtg atattttgcc cgttcacgcc ctgctgtagg gttgacgatt
    13261 aacatacacc gtttcaactt gctctactcc ttacttgtaa attgtgataa gtccttaacg
    13321 tgactgatga ttactattat agcggtaaaa aagtcgttcg tgaaggtgta aacaaaggat
    13381 ttctctgatt ttcgtcgaac tctttaagta atgttcactt gtaatttttt gaaaagggga
    13441 gattggaatg aattttgatg catctgtgta tccctatggc tcccaccggg aagtggtcta
    13501 tggccgccgg ggcatggtct gcacgtctca gaccctggct gcccaggccg ggctggatat
    13561 gctgaaaaaa ggcggcaacg ccatcgacgc tgccctggct gcggccatgt gcctgactgt
    13621 cctggagccg accagcaacg gcctgggcag cgacgtcttc gccctgatat ggacgaaggg
    13681 gaaactctat ggcatcaacg gcagcggctg gtccccggca ggactgacgc gggatgaatt
    13741 gctgaagcgg ggctatgacc aggtcccgct ccggggctgg gagccggtca tggtcccagg
    13801 ggcgccggcg gcgtgggccg aagtccatcg ccgttttggc cgactggcct ttgaagaact
    13861 ctttgaaccg gccattgcct atgcccggga tggttatgcg gtcatgccga acctggcagc
    13921 tatgctggcc gacagtatgg catcctttgc accttatcgg gggaagaaaa tctttcagca
    13981 cttattcgat acttttttcc ctcatggcca ggcgcccaaa gccggtgata tcctcaaatt
    14041 gccggaactg gccaagtctc tggaaatcct gcgggacagc cattgccggg ccctgtacga
    14101 cggtgaactg gctgacgcca tagagaactg gtcaagggaa acgggcggtc ttatccgaaa
    14161 ggcggattta gcagcgtacc agccgcagtg ggccgacccg atccatacgg cgtaccgtgg
    14221 ttacgatgtc tgggaaatgc cgcccaacgg ccatggcctg gtcgtcctca tggccctgga
    14281 tattgccagc gggtttactt ttagtaaacg ggatgatgcc gaatcgattc accgccagat
    14341 cgaagccatg aaactggcct tctgcgatgg caagaaatat attgccgacc cgcgtttcat
    14401 gaagacggct gttgacgatt ggctctctcc agcctatgcc gaccggcggc gggcccttat
    14461 cggtcacgag gccctgctgc cgcagccggt cgatgtaaac tgtggcggta ccgtttactt
    14521 gtgcagtgcc gacggcgaag gcaatatggt ttctttcatc cagagcaact acagcggttt
    14581 cggttcgggc atcgccgttc cgggatacgg catttctctc agcgacaggg gtcataactt
    14641 ctctatggac ccagccagcg ataactgcgt cgggccgcgg aagaagtcct atcatacgat
    14701 cattcccggc tttttgacga aagacggccg tcctgtcggc cccttcggcg tcatgggcgc
    14761 tttcatgcag ccccaggggc agttccaggt cctgctgaat accatcgact atcatctcaa
    14821 ccctcaggca gccctggatg ctccgcgctg gcagtgggtc ggcggcaaga ccattgaaat
    14881 ggaacgggcc tttccaaccg ctgtcattga agaactgcgc cgccgcggcc atgacgtcgt
    14941 cgtcaaagac gatatccaga cctatggccg gggacagatc atctggcgcg atgaccaggg
    15001 gacgctcatg ggtgggaccg aaccccgggc cgatggctgt gtcgccgcct ggtaaaggtc
    15061 gtttactttt cttcttccgg aaaatatgat aggatggaga aaactaaagg aggactgtcc
    15121 ccatgaaacc gaaactttct attttagacc ctaatccgac gccgctccaa tacctgtccg
    15181 gcctgtcgca ggaattattc cgtgaaatct atatcaagcg cgatgacctg acgcctttcg
    15241 gcggcggtgg caataaactg cgtaaattag agtatctgat gatcgaagcc ctcaactctc
    15301 aggcgacgac cattgtgacc gtcggagcgc cgcagaccaa ccatggccgc ctgacggcgg
    15361 ctgttgccgc gaaattcggc ctgaaatgca ttatcgtcgc tgtcggtgat actgatggcg
    15421 aactgagcgg caatttactc ctcgacggta tttttggcgc ccgcgtcgtc ctcaagcatg
    15481 acgatggccg cgaccacgac gtccagctcg aagaaacagt caagaaagtc atggccgccg
    15541 aattaaagaa gggtgaacaa gtttacttca tccccatggg cggcagcgat acgacgggta
    15601 tgctgggcta catggactgc gcccgcgaac tcgacgccca ggccaaggaa cagcagatcg
    15661 acggggcgac ggtctatgtc gccgtcggca gtatgggtac atatctcggc ttgtattgcg
    15721 gcttgaaagc cattcagtcg aatttgaaac tggtaggcat tgccgtcatg cccttcgaca
    15781 tggaaaaatt aaaggcctac tttaaccagg ccaaggaaga atatggcttt gatttcgaca
    15841 gcgacttcca cgtcgaaacg ggctatatcg gcaagggcta caacgaaccg gaaccgcggg
    15901 tccgcgaagc catctacacc atggcccgtc acgaaggcat cctcctcgac ccgtgctaca
    15961 cgggcaagat gtttgccggc gtcctgtcga tgatcaaaga aaagaaaatc aaactgggcc
    16021 ggcaggtcat cctcgtgcat accggcggca tgcctggcct ctacaccaaa gcccatcgca
    16081 tcaaatttga aaacgaactc aaaaaccaga tcgagattgt gaagtaaagg gctagtggtt
    16141 agcagtcagc agttagtggc tcgtggctcg tggctagtgg ctcgtagggg acgcccaaaa
    16201 cggacagcag cgatgggcgt cccgttagga atttggctga ctctcttagt accctgacag
    16261 ggaaataaat ttaaaaccat ccgctaacca ctaactacta gccactaacc acttaaaagg
    16321 ggctgtcgca tatgcgacag ccccttttac tatgttttta aaagaacacc tttgtcaata
    16381 tgacgtacga caaggcaccc atgacagctg tgcagggaat ggtcatgacc caggccgtga
    16441 ccatctgccg ggcgacgctc cagtggacgg ctttgacgcg ctgtgccgaa ccgacaccca
    16501 tgatggatcc ggaaacgacg tgtgtcgtcg agacgggcag gtgcagcagg gtggccgaga
    16561 agatgacgac cgctgagttc aagtcggctg caaagccgtg gacgggatgc atcttgaata
    16621 tcttgccgcc gacggtacgg atgattttcc aaccgccgac gctggtcccc agggccatgg
    16681 acagggcgca gaggaatttg accaggtccg gtacttccag gctggtgatg aagccgcccg
    16741 ataccaatgc cagggtgatg atacccatgg atttctgggc atcattggag ccgtgggaaa
    16801 aagccatgag ggccgccgac acgacttgca gcctgttggc cgccaggttt accttggccg
    16861 gtttgaatga atggcagacg gcaaagatga tcttcatgat gatgaagccc gtaatgatgg
    16921 ccgaaaccgg tgaaatgatg agggacagga cgattttcag gataccccag ccgttgaggg
    16981 cggctacacc ggccgatatg atgatggcac cgatcatgcc gccgatgagg gcgtgagacg
    17041 aactggaagg catgccgacc caccaggtca ggaggttcca gatgatagcg ccgaccaggc
    17101 cggcgatgat gatcatttcg ttgactaacg tcggcgagac gacgatgtcg ccgccaatcg
    17161 tcttggcgac acctgtcgaa atcatggcgc cgaggaagtt gaggatagcc gtaccgacga
    17221 tggcgacgcc agggcggatg gcacgtgtcg atacgcaggt tgcgatggca ttggccgtat
    17281 cgtggaagcc gttgatgaag tcaaagacca gagccaggac gacgaccatg atgatgagcg
    17341 acctatcaag catatttcat ggtcacctct ttaatggtat ttcccagggt ttcgacgtgg
    17401 tcggccgctt cttccagggt tcctaagata tctttccagc ggatgacgtt gagcaggtcc
    17461 gtttcatcgc cgctgaagag cctggaaatt tcctggcgat agatatggtc tacttccgat
    17521 tcgaggcggt tcagtcgttc agcatgacgc atgagtttgg cttcgttgtg gtcgatatcc
    17581 tttaggaaag agaaaatctt cttcaattct tcgcccatag tgacaatctg ttcggtcatg
    17641 gcgatggccg ctttcggcgc ttccgtgacg tgatacatgc tggtgcgcat gagagcgccc
    17701 tgcagggaat caatgcagtc ttcgagctga cacgtcagtt tgtaaaaatc ttcccggtcg
    17761 ataggcgtga tgaaaacgcg ggacaatttg aagatgactt cctggttggt tttgtccgac
    17821 gtgtgttcca gtttgacgat ttccttgaga tgcatggaaa tcgttgaaac gtcctccatg
    17881 acttcatggg cgatgagggc gccgcgatgg aaattttcgg cgttggtcat gagataatcg
    17941 aagaattctt cgtgcttgtt gctgatactg aacatgttat ttcctcctct gtgtacttaa
    18001 aatcagtata tgagagacat atctttgttt tgaaaagaat atgtaaagat tgagtaaatt
    18061 ttagctacat gttttatttt ttttgagtca atggtataat aaaaacggta ttgttatttt
    18121 ttataggtat gtatattgtt aagaaaaaat cgcttgccat ggtgcaggct ttttttgaac
    18181 atgaggtgac ttttacgaaa tgaacgaaaa cctgactaaa cggatccatc ccgttgacga
    18241 aatgctgccg gctggccggc tctttgccta cggtctgcaa cacgtcctgg ccatgtacgc
    18301 cggggctgta gccgtaccga tcatcattgc ccagaccctg cacttgtcgc cggctgaact
    18361 ggtccacctg atcaatgccg acttattttc ctgcggcatc gccacgctga tacagacttt
    18421 aggcttctgg aaaatcggcg cccgcatccc gatgatccag ggggtcactt ttgcgtcggt
    18481 cacgccgatg atcctcatcg gggcggaaca cggaattacg gccatttacg gatcgattct
    18541 cattgccggg ctgttcactt tcttcattgc tcctttcttt agccggttca tccgcttttt
    18601 cccgcctgtc gtcacgggga cgatcatcac catcatcggt atcaccctgc tgccggtagc
    18661 cgtcaactgg atgggcggcg gcaatccgaa gtcgccggac ttcgcctcac cgatgaattt
    18721 gttcctggcc ttcatcaccc tggtcatcat catcgccatt taccgctgcg gcaagggctt
    18781 cttgagcaat gtttctgtcc tgctgggact catcggcggg acgatcattg ccatgatttt
    18841 agggcagacg aacttcgccg aagtcggcaa cgcggcctgg attgggttag tcacgccttt
    18901 ttccttcggc ttcccgactt ttgatatcgg ctcgatcctg tcgatgatcg tcgtcatgat
    18961 cgtcacgatg gtagaaacga cgggggactg catcgctatc gggtccatcg tcggccgccc
    19021 cattggccgc agccgcctgg cacgctgcct ccgggctgat ggcttatcga cctttatcgg
    19081 cggtatcctc aacagcttcc cttatacggc ctttgcccag aacgtcggcc tcatcgccgt
    19141 cacccgcgtc aagagccgtt tcgtcgtcgc cgcgtctggc atcatcctca tcgctttggg
    19201 cctgttcccg aagatggcgg ctgtcgtcgc ctctatcccg aattccgtct taggcggtgc
    19261 aggcgtagcc atgttcggca tggtcattgc cagcggtatc cgggccttga gtaaagttaa
    19321 attcgacggg acgtacaatc tgatgattgt cgccgtcagt atcggcgtca gcatgatcac
    19381 cctgacggcg ccgaactttt tccacagctt cccgtcgtgg gcgaacatcg tcctgcacag
    19441 tggcattacc ctgggcagta ttacggctgt cgtcctcaac ctgattctga atggcagcgg
    19501 ccgggaagat gatgtaaacg aagattaaat aaatgtaaat caaaataaag gcgcatcagc
    19561 gcgatgaaag tgagggattt ttttgctgaa acgaatgatg gctctgacct gcgtcctggc
    19621 cttgaccgct ggagctgcct gggccgatac gccggaactg aagactggac cggctccttt
    19681 tgcagtcaaa gtggaaatca agccgacggc ggcgccgcag gaagaaacga aaccggccca
    19741 gccagccgta acggcgaaga aggacacgaa aagccagacg gccgccaagg aaggccagca
    19801 ggccaaggcg gtcgaatcgg aaaagaaaat aggtatcaac ctggccagcc gtatcttaac
    19861 gctctatgaa ggcgatacga aggtcaggat gtatcacgtc ggggtcggca agacgtcgac
    19921 gccgacgccg accggttact atgccgtcca atataaggaa gtcaacccga cctgggtcga
    19981 cccggatgac acgagcatcc agatcggttc aggtccggac aaccctattg gctaccgctg
    20041 gatcggcttt tacggtaact atgggatcca tgggaccaat catcctgaat ccatcggcgg
    20101 ctatgtatcc aatggctgcg tgcgcatgaa agaagccgat gtagaagacc tctaccagta
    20161 tgtatccgtc gggacgccgg ttacggtcta ctatgaccgc ctggtcatcg acgtcgaccc
    20221 ggaccatacg gtttcttatt atatctatcc tgacggctac ggctggcagt ccctgagcat
    20281 cgctcaggtc aagaaggccc tggccggcta tggcgtagaa gattttgctg atttccagga
    20341 aatctccgat aagatcaacg cctccgatgg caacgtcacg tacatcgcca aggcttacga
    20401 tttagttgta aacggccata aactggccaa gcgggcactg ggcaagaatg gccagattta
    20461 cctgccgtcc gtagccgtgg cgacggcgct gaatctggat ttacagtgga attcccagca
    20521 gggcatcctg accagtcctt atggcatcgc cccgggatac gtcaagagcg acgtcgttta
    20581 catgaacgcc gtcgacgcct actccctgtt ccatctcaaa ggtgaactga cgccggacta
    20641 tgtctataat atgtactccg tcaaaggcaa cagtacaccg accgtcgtca tctcgccggg
    20701 aagcgggaat gattgacgtt tgatgcgatg gcttttttgt aggggcttac acgtgctttt
    20761 ttagcccgtc aggtatatgc aagatggcca tgcaccgatt ggcgtgaacg ggcgccccac
    20821 gtgggcgccc ctacgacgaa cgataaacga cctacgatga actcaaaaag aggctgtctt
    20881 aatgagacag cctctttttc attgacaaat actgacatat gtcatatact aaaatcaaat
    20941 taatcgacat atgtcaaaaa aagacgggcc gcgaggaccc gtctggttat tgcagattgg
    21001 aggagaaatt gttatgatga gagtagcagt tacgtatgaa aatgggaatg tattccagca
    21061 tttcggccat tcggaaaaat tcaagattta tgatgtcgaa gatgggcata tcgtcaaggc
    21121 tgacgtcatc gacaccaaag gtcagggcca tggtgccctg gtcggtttcc tgcaggccca
    21181 ggaaatcaat gccgtcattg ccggcggcat cggtggcgga gcccagcagg ccctggcggc
    21241 cgcttcgatt gacctctatg gcggcgttac gggcagtgcc gatgaagccg tcaaggattt
    21301 gctcaatggt acgctgaaat tcaatcccga cgtagcttgc agccatcacg accagcagca
    21361 tggccatggt gaacatcact gcggttccca cggctgcgga ggtcatcagt aacctgtctg
    21421 ttccctggaa aggagacgat tcccttgaag aatatcaccg ctttttatat gaacggctgc
    21481 ccctattgca agggcgccaa agaagccttg gctgaactga tcgaagaaaa tccggcttac
    21541 cgccaggtag ccgtcgactg gtacgaagaa tcggaacacc ccgaagtcgt acagggccat
    21601 tcttactact atgtgccgtc tatgttcatc ggcacggaaa aactctacga agcccagcca
    21661 ggccagtctt atgacgacat caaggcccat gtcaaagcgg ccctggatgc ggcgctgaag
    21721 gcttaatcgc cacctaagac ggcatggacg gcatcccggc agcggcggac ggcttcatcc
    21781 atggtctgct catcaatggt gaggggcgga ttgaagtaaa tgacgtcgcc aatggggcgc
    21841 aggaccagtc ccaggtccat ggctttgcgg aagatgtgcc agccgacccg cttttccggg
    21901 tcaaaggcgc gtttactccg tttatcttca actaattcaa tcgcattgat caggccgatg
    21961 tggcggattt cgccgacatg gctgtggctg ccgagagcag cgtctaactt ttcgtgaagg
    22021 tagatgctct tttcggcagc tttttttaat atatcgtcgc gttccatgat gtgtagcaca
    22081 gccagggccg cccggcaggc caggggattg ccggcatagg tgtggctgtg gacgaaagcc
    22141 ttatgggtct tgaaatcgtc gtagaaggcc tggtaaatgg catcggtcgt aatcgtcagg
    22201 gacatgggca ggtagccgcc ggtcaggcct ttcgacgtgc agaggatatc cgggctgacg
    22261 ccggcgtgtt caatggcgaa catcttgccc gtccggccga agccggcagc gacttcatcg
    22321 tcgatgagca ggacgccgta ctggtcacag agctggcgca ggcggcgcag gtattctggc
    22381 gggtagatgc gcatacccgc cgcgccttgc agaatcggtt cgacgatgag ggctgccgtt
    22441 tctttgccgt aagcggcgaa ggccttttca gcgccgacga aacattcgca gtcacacgta
    22501 tcccgcgttt ggccataggg acagcggtag cagtcgaggc cgtcgaaatg gatgttattc
    22561 atcatcatgg gcttgtaaat gcgggcatag aggtccatac tgccgacgga cagggcgccg
    22621 atggtctcgc cgggatacga ttcagacagg cacatgaaac gctgccgttc agggtggccc
    22681 gactgatact gatattgaaa ggccatcttc agggcacatt cgacagacga tgagccattg
    22741 tcggtgaaat tgaaatgcgt cagccctttt ggcagcagcg gcagcaacgc ctgacagagc
    22801 tcgatggccg ggcgatggga gaaattggca aagatgacgt gttccagctg gtcgagctgg
    22861 tctttgatgg cgtcattgat ttcgtcgttg cagtggccga ggagattgca ccaccaggaa
    22921 ctgatgatgt cgatgtattc tttgccttct gtgtcataga gatagcagcc tttgccgtgg
    22981 tcgatgacga cgggcgggaa gacttcggcg tctttcatct gctgagccgg atgccagatg
    23041 tattttaagt cagcttcaag taaactcatg taaatcactc cttactggta tagttgaatc
    23101 aaagcctggg catcggcctg taaaagttcg gcgttgtcgc cgacgacggc caggacgggc
    23161 ttgccgctga gcttttcaat catgcggatg ttgtcttttt ccatggtgct tcccgtataa
    23221 tggttgacga tgaaaccttg gacaggcagg cccttttctt tcatataaaa ggcggtcagg
    23281 acggcgccgt tgatggtccc caggccagcc gggcagacga tgagcgatgg gatgtgcagc
    23341 cagccgatga gatcttccag ctggaagctg atggcatcat cttcgcggag gggacagatg
    23401 atgccaccgc tgccttcgac cgtgacgtag ggataggctg ccgtgacgcg ctgccagtcg
    23461 cggaggatga cgcttttttc gatgggcttc tcttcccagc gggctgccag gtgaggcgac
    23521 acggcgtggt cgtaaagata agacaagagc aggtccggcg attcgccaat cccggctatc
    23581 tcatttacat aaccggcatc actggccgcg acggtcgggg cgccgctgat ggcagcctta
    23641 taataaccga cgtcataacc gtgctggcgc agtgtcttga tgagcagggc tgtgacatag
    23701 gtcttgccga tgtccgtccc cgtcccggtg atgaataaac ctttactcat agtaaacgaa
    23761 ctcctttcta gagggcagtg gtccgacgta ttcggcgggt caggaaggcg gcgacgatgc
    23821 agagaaagat gtcaccggga atgggcagga cgaagcagta gacgaagagt gtccacagac
    23881 tgatcggtgt gcccaggtat acgttattga ttaaagctaa atagaccatg ccgcaggcgt
    23941 agacgatgat gaggccggca aaatcagcag ccagcaggcg ctgcagcgat ggcatggcgc
    24001 tttgatgggc aatgtatccg gtcagccagg caccgccgat gaagccgatg agatagccga
    24061 aggtcggctg gaaaatatag gacggaccgc cgccagacgt gaagaccggc agtccgacca
    24121 gccctaagag gacgtagacg gccaccgagg cggcgccgcg gcggctgccc aggagcagtc
    24181 cggccagggt ggtgaacaat aattgcagcg taaaaggaca gaacggaaca gggatgcgga
    24241 taaaggcccc gatggcgatg agcgccgtga acagggcgaa taagaccata tcccgcgtat
    24301 cccagtggtg ggctgagagg acagaagaaa ccatgaaatc actcctttgg ataaatgtaa
    24361 accacaataa tatttaaaaa gtttacgata atctggatta tagccaataa tcgacttatt
    24421 gtcaattttt aaatagtaca aattagttaa caataatccg gtgagaattt ctcccattga
    24481 caaaagcgaa agaagtgatt aatctataat tacaaaatag atataaatta ttttgtttaa
    24541 aaaagaaatg gaggaattgc gatgtcaaat gttaatattg actggtcggc cttaggtttt
    24601 gaatatcagc cgattgcaaa acgttatgtt gccaactata aagatggcaa atggggcaaa
    24661 ggggaattga cggataaccc gaatgtcgtc ctcaatgaat gtgccggcat cctgcaatac
    24721 tgccagcaag tctttnnnnn nnnnngccta taaaaccaaa gacggccgca cggtcacctt
    24781 ccgtccggac cagaacgcaa aacgtttgat ggactcggca aaacgcttgg aaatgccgcc
    24841 ggttccggaa gatatgttca tggaagctgt cgacgaagtc gtccgggcca ataaggatta
    24901 tattcctccc tttgaaagcg gcggcgctct ctacctgcgt ccgtatctct ttgggactaa
    24961 cccggtcatc ggcgtcaaac cggctgacga atatcagttc cgcctcttcg gcacgcctgt
    25021 cggctcttac ttcaagaacg gcgtcaaacc gattacgatc tgcatttctg acttcgaccg
    25081 tgcagcaccg cgcggcacag gccatatcaa agccggcttg aactatgcca tgagcatgta
    25141 tccttacatt ttggctcatc agaacggctt cgatgaaaac atgttcctcg acccggctac
    25201 gcgcacgtac gtagaagaaa cgggcggcgc taacttcctc ttcgtcaaga aagatggcac
    25261 tttggttacg ccgaaatcgg actccatcct gccgtccatt acccgccgtt ccctgatcac
    25321 gatcgcaatg gacatgcttg gcatgaaagt cgaacaccgt ccggttaagt tcgcagaagt
    25381 cagcgaattc gctgaagccg gcctctgcgg tacggctgcc gtcatctgcc cggtaggcaa
    25441 agtcgttgac cacggcaaag aaatctgcct ccccagcggc atggacgaaa tgggtcctgt
    25501 cctgacgaaa ctgtacaaga ccttgcgggg catccagatg ggcgaaatcg aaggaccgga
    25561 aggctggatc cacgaagtta aataatttat atttcgtagg ggctcgcacg tggcgagccc
    25621 gcgggtatat gtaagatgcc cgtgaaccga tttgcgcgaa cgggcgcccc acgtggcgcc
    25681 cctacagaaa aaaggcttgt cgcatgtgcg acaagccttt tttttacaac acgcccaagc
    25741 cttccagaaa tatttttaaa ccaatcaaga tgaggacggc cccgccgatg cgttcggcct
    25801 tatttttata gcgcatcccg aagaggctgc cgacttttat cccggctgcc gagaacagga
    25861 acgtcgtcac gccgatgagc gtcacagacg acacgagggc gatgtccata aaggcgaagg
    25921 tgacgccgac ggccagggcg tcgatactgg ttgcgatagc cagcatgagc atcgtatagg
    25981 gactcatgga cgcatcacag tgctcttcgt cctcaaagga ctccttaatc atattgaggc
    26041 cgatggcgct gagcaggatg aacgtgaccc agtgatcata ggcatcgatc atgtcggcga
    26101 aaaagctgcc cagaccatag ccgatagtgg gcatcagagc ctggaagccg ccaaaccaca
    26161 ggccggccag gaccatgtgg cgccagttga tttttcccag cgacaggcct ttacagatgg
    26221 aaacggcaaa ggcatccatg gataggctga cggccagcaa gagtaaatca agtgtattca
    26281 ttctcttctc tcctcatcaa tgcagcaata tacaattcaa ttattatagg agatagggaa
    26341 atcgatgtca acgaaaaaga ttgccaaatc aacgaattgt gctacaatac attttgtgac
    26401 aagaaaggac gtgaaaacac atgaaatata gaaagcgcca tctcctggcg gcgctcctca
    26461 gtgcgtttac tttattttcc ggtcaggcca tggccatgtc cgtggataac ctgatgcagg
    26521 tcgtcgccaa ggacagtgcg ataggcaaga ccatttcctg gcagtatgac agcgaccgta
    26581 gcgattacag tgtagaatac cgtcacaagg accagggtga tgtccagttg gcttatgtga
    26641 ccgaagggaa acggcctccc atttacgacg ccgacaatcc ggcaccctat acgtatgggg
    26701 cttacatgca gaaactgaca ccggcgacgg aatatgaata ccgcatcgtc aatgctgacg
    26761 gtgcgaccga ttggattact ttttcgacga caaaagaaga tttaaacaaa tacaaagtcc
    26821 tcgtcttcgg cgactctcag tccaccgatt acagcgtctg ggggcagacg gcgcagatcg
    26881 cctggcagca gaacgatgat gcggctttct tcatcaacat gggcgacatt accgacaacg
    26941 gacaggccta cttccaatgg cgggactggt ttgaaaatgc cgatgtcctg accagccaca
    27001 tcccttttgc cccggtcctg ggcaaccacg aagcctatag catggattgg aaatttgccg
    27061 aaccgtatac gtataaggcc ctgttcgccg tgccctatgg tggaccgaag gaccagaacc
    27121 gcctggctta ctccttcgat tatggcgacg tccactacgt gtccctcaat acggattatg
    27181 aagaattgaa cggctggcgt ccgacgatga tggaagatga agcagcctgg ctcgacaagg
    27241 atttggctgc ggccaagaag gccgggaaac ggctggtcgt cctcatgcac cgtccgcctt
    27301 gggacagccc gtataacggc accctcgatg taaacggcaa atacttcatg ccgctcttcg
    27361 ataaatacga cgtccccctg gtatttacgg ctcatgaaca ctgctatgaa cggaccgttc
    27421 ccatcaagaa cgaccaggcc gctacggaag ggaccgtcta cattgccacg ggccgctccg
    27481 ggacagaagc ctgggatggc tcggtcaaga agccgactga tgtcatttac tataatccaa
    27541 tggatatgcc catgtacctg acgctccagg tcgaaccgga tgaattccgc gtcagcgcca
    27601 tgaaaaatga cggcaccctg atcgataccg tttccatccc ggccaagcac caggaagcgg
    27661 aagtttccaa aaacgcagac aacaccgtaa aggcgccgga aacgaagacc aagaaagata
    27721 agcagagtgc taagcaacat gctaagaaaa gtgtaaacaa acataaacag aagtaaagac
    27781 ggttgttttc tttctggaag atgtgtataa ttaacagtta cagaaagtaa acagaagagg
    27841 agcgaatgag aatgaaaaaa ttagcaaaaa atatgatcct ttgtgcgctc gtgacgatga
    27901 gtttatcagc cgtctgcttt gcagaagata cgactacgac ggaaacgacg gcaacagaac
    27961 cgcaggcaac agctacggtg acgacaacga ctcaggccgc tccggaccag aatgtcgtcg
    28021 actggctcga cccggctgcc ggtgaatggt acagcaccaa gggcaacctg gtcatgacca
    28081 tcgaaggcaa caccatcaac ggctgtacgg taacggaccc gaaagactgc acctatgact
    28141 atccgcggac cgggaccttc accatccatg aacagactgg cgaccggacg atcaagatgg
    28201 acctcatggg ccataaatcc catcagtacc tcatcgtaga caataagatg cccttacgcc
    28261 gttccctcaa cgccgaccgt tacgaatcca ttggcggcgt ctacctcggc atgacccagg
    28321 aagacctgac ggctgcctat ggccagccga gcagtactgc cgaagatcag ggaacagacc
    28381 gctggattta tgataaccat cacatggatg cttatctcca gggcggcatt gtcatcggta
    28441 tccgcattta tgacggcagc gatttgaagt tcgataaatc tggcctgacc gcttctgata
    28501 cagcaggtgc ctatgccaaa tcttacgaat tggaaacgac gccggaagtc ccgacagaag
    28561 ccaacgccat ttccaaagcc tataaac
//
LOCUS       DAWABZ010000009        28389 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_479_length_28389_cov_3.268158, whole genome shotgun sequence.
ACCESSION   DAWABZ010000009 DAWABZ010000000
VERSION     DAWABZ010000009.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 28389)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 28389)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..28389
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_479_length_28389_cov_3.268158"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5869..5878
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9764..9773
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    22260..22269
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6721 a         8162 c         7587 g         5889 t
ORIGIN      
        1 agcatcacct tgccaaggtg ggggtcgcga gttcgagtct cgttttccgc tccatatatg
       61 gcggcatagc caagcggtaa ggcagaggtc tgcaaaacct tcatccccag ttcgattctg
      121 ggtgccgcct ccatattttc atctttcgtc atgccgaggt ggcggaactg gcagacgcaa
      181 cggacttaaa atccgtcgga tagagatatc cgtaccggtt cgattccggt ccccggcacc
      241 aaaaaaacaa cgagcgtgtg agcggcgttg gccgtggccg acgagtccct tgcaaaagaa
      301 aggagctgtc ttaggacagc tccttttcgc agtccagtga aattcacatt catgggaaaa
      361 tgagttataa tgaatcatga cagaatacaa gtataaagta tcggattttg aaggtcccct
      421 ggacctgctg ctctatctca tcgaaaagaa caaagtcgat atttataaca tacctatcgt
      481 cgagattaca gaccagttca acgcctacgt agcggacatc gacacattcg atgtcgatta
      541 tgccagcaag ttcttcgtca tggcagcgac gctgttgcag atcaaatcgc gcctgctgct
      601 gcctaagcag ccgaaactgg ctgaagagga ggaagaagac ccgcaggaag cgctggtccg
      661 ccagctcgtc gaatataaaa agatgaaagc cctgtcccag gctatgggac agttgtggga
      721 tgcccgcagc ctcatgctgg ggcgcctgcc gacggccatg ccctatgagg cccgctttgc
      781 cggccacatc gacacagagg ccctctacca ggcgttccac aaggtctgcc aggcccttcc
      841 cgagccgaca gccgtagaaa tccgcataca gaaggaaatc tacagcgtcg aagagtgcat
      901 gcgccgcgtc acgtacgccc tgcgccgtgc ccaggggacg atggccgtcg acgccatatt
      961 ccggacctgc cggtcccggg tcgaactggt cgtcaccttc ctggccgtcc tggaactgct
     1021 gaaagttggc cagtgcgcta ctgttgctga aggagaggat gggaatacca ttgccttacg
     1081 atacacgcca aactgatacc atcgagccta cggctctgct ggaagctctg ctctttctga
     1141 gcggcgaccc catgacggtg acggccatct gcgcccatac gggctggacc caggccgaaa
     1201 cggaagcgac ggcagaccgc ctgcagcacc tgctcagcag ccagcggcgg ggtatcgtcc
     1261 tcatccgcgt cgccggcggc tttcagctgg tcacgcggcc ggacctccac gatgccctgc
     1321 aatgggtccg gacggggacg acggagttgt cctctatggc actggaagta ttggccatcg
     1381 tcgccttcaa acagcccatt acgcgggctg aaatcgaaaa gctgcgcggt gtctcgtcag
     1441 aacgggtcct gacgtcgctg gtccagcagg gcctcgtcgt cgaactgggg cgcaaggaca
     1501 gcccagggcg gccgatttta tacggcacgt ctgcttattt tttagaatgt atcggcatgg
     1561 attccctggc cgatttagcc ggccgcatgc cggcagatat tacggaggat gcaactgatg
     1621 gaagaacggt tacagaaagt gatgagtaac tgcggtgtcg cttcgcggcg cgcttcggaa
     1681 aagatgatat tagaagggcg cgtccgcgtc aacggcgtca tcgtccgcga actggggacg
     1741 aaagtcgacc ccgacaagga ccgcattgcc gtcgacggca agaccctgga agcgcagccg
     1801 aagcattatt atctgttcaa caagcccaag ggcgtcatca ccacgtcgag cgatgaccag
     1861 ggccgggcga cggtcatgga ctatttcagg aaggaaaagg accgcatcta cgccgtcggc
     1921 cgcctggacc agaatacgga aggcctgctg atcatgacca atgacggtga actggccaac
     1981 atcctcatgc atcccagccg cctcgtcgac aagacttatg aagtcaaggt caaagggcgc
     2041 attgccgatt cggtcctgca gcacctggca gacggcgtcg agctgaagga cggcatgacg
     2101 gccccggccg atgtctacta tattggctat gacccgaaga cgaacctgac cagtgtggaa
     2161 atcaccatcc acgaaggacg caaccgccag gtccgccgca tgttcgagtt cttcggctac
     2221 caggtccaca acctgcgccg cgtccagtac gccttcctga ccctgagcgg cgtcaagcgc
     2281 ggggcctatc gcaagctgac ccgcgaagaa gtggcggaac tctacaaata taaataaaca
     2341 ggagtgctta tgaaggaaat actcgtcatc ggtgccggtg cggccggcat tatggctgcg
     2401 ctggccgcgg cagaagccgg ggccagggta cacttatttg aaaaaaatga tatcgtcggc
     2461 aagaagatgg gcattaccgg caagggccgc tgcaatctga ccaattcgtg cagcatggcc
     2521 gatttcatcg cccatacgcc gggccacggc aaattcctct acagtgcgta tgaacaattt
     2581 accaatcagg atctgctgga taaattgcat acctggggcc tggctaccaa ggaggaacgg
     2641 ggcggccgca tcttccccca gtcggacagt gccatcgaag tgcgcaagct cttctatcgc
     2701 aagctctgcc agaagggcgt cgacctccat ctgagcaacg ccgtccacgc cgtcaaggcc
     2761 tggggcagcc gcatggtcgt cagtgcggcg tcggggaatt acgaaggcga cgcctgtatc
     2821 atcacgacgg gcggcatgtc ctatcccgtg accggttcga caggcgacgg ctacggcttt
     2881 gcccgcagcc tgggacacag cgtgacggag ctgaaaccgt cgctcattcc ctttacgacg
     2941 gaagaaacct ggcctcacac cctgtcgggc ctgtccctgc gcaatgtcga agcgtcgctg
     3001 tggaagcggg gcaagaaagc tgcctctcat ttcggcgaga tgctcttcac tcatttcggc
     3061 gtgtccgggc cgatcatcct catgctcagt accgaggcgg cccataagaa gtcctgcgtt
     3121 tttcccatgc agctgcgcat cgacctcaaa ccggctttgt ccaaggaaaa gctcgacgcc
     3181 cggctgcggc gggatttcga gaaatacatc cgcaaagaag ccgccaatgg gctgaaggac
     3241 ctcctgccgc agcggctcat ccccatcgtc ctcgaccagg ccggcatcgc cgctgcctgt
     3301 ccggtcaacc agatttcccg ggaacagcgg caggacctgg tcgataccct caaggccctg
     3361 agcctgaccg tgaccgggac gcgtcccatt gaagaagcca tcgtcacggc tggcggcatt
     3421 gccgtcaagg aaatcaatcc caagatgatg gagtcgaaac ttgtccccaa tctctatttt
     3481 gccggagaag tcatcgacat cgacgccttt acaggcggct ataatctgca ggcagccttt
     3541 tcgacgggct tcgtcgccgg aacggcggcc gctacaaagg agtgcatacc atgcgaaaat
     3601 tgacggttgc cattgatggg ccggccggag cgggcaagag cagtgtcgcc aaggtcctgg
     3661 ccaaccgggt ccattacttg tatatcgata cgggtgccat gtaccgggcc tttacctggg
     3721 ccgtcctgga aaaaggcatc gacctttctg atgaaaaagc ggtccgtgac ctcgtcgata
     3781 ccattgatat ccgcctggaa ccgcaggcag acctgtgccg cgtctacgtc ggaccgacgg
     3841 aagtgaccga agccatccgg acccagcgcg tatcttccca tgtttctgcc gtcgcggctc
     3901 ttgcagtcgt acgggaaaaa ctggtaaaat tacaacaggc catggccaaa gaaggcggcg
     3961 tcatcctcga cggccgcgat atcgggactg tcgtcctgcc ccaggcagac ctcaagatct
     4021 tcctgacggc gtctgtcgat tcccgggccc gccgccgtta tttggaagtc caggccaaag
     4081 gtggcagcga gacgtatgaa gaaatcgctg ccagtatcgc cgcccgcgac gatatggatt
     4141 cgcaccgcac cgtttcgccg ctgaagaagg ccgacgatgc catcgtcgtc gacaacagcg
     4201 atctcgattt agagcagacg gccgacgtca tcctgggcct gatgaaggag cgaggctgat
     4261 gcataccttt acatatggcg tgatctgcaa gattttcaat gtcgctacct acggcgtcct
     4321 gggcatggaa gtcatcggtg aagagaacat ccccgaaaag gggccgttcc tggttgtctg
     4381 caatcatgcc agcaatttcg atccgctcct gctggggacg gccatgcgcc accatctgat
     4441 ccatttcatg gctaaagagg aattgttccg caatcccctc atgggctggt tcctgcgcta
     4501 tgtccacacc ttcccggtcc accgcggcca catcgaccgc aaagccgtca tcgagtcctt
     4561 tcgggtcttg aagagcggcg aggtcctggg gattttccct gaagggaagc ggacgcgggg
     4621 cggcaaagtc gggcctttcc atgaaggctt tgccggaatc gccatcaagg ccaaggtgcc
     4681 ggtcctgccg gcagctatcg tcaattccga attcctgccg aaaaagaccg gtcccgtccg
     4741 ggtcatcttt ggcaaacccg tcccggcacc gcagggaaag gcatcggatc gcgacttggt
     4801 caaaggtttt tccgacgaaa tccgcgatat tatcatagct atgcaaaatc agtataaagg
     4861 ggacgcaaaa tgaaagtaac tctcgccgcg gcctgtggct tttgctatgg tgtgcgccgg
     4921 gctgtcgagc ttgcggggca ggccgaaaag gggacgcata ccctggggcc catcatccac
     4981 aatccgcaag ttgtcgggcg tttagccgct catggcgtat cgccggtcga ctcgctggac
     5041 gatgtcgaca agggggctgt cgtcctgata cgctctcacg gagtcggccc ggatgtctat
     5101 gaacaggctg accaaaaaga actaaaagtc atagatgcga cgtgtccaca cgtcaaaaag
     5161 gcccaacaag acgctaaaaa catcgtcgaa aagggcgaaa aactcataat agtaggagaa
     5221 aaatcccatc ctgaagtaat tagcatttca caatgggggg caaatcatgc tattataata
     5281 gatggggaag aagaagctga aaaaattccc ttttgcgatt ctatgggagt cgtcgtccag
     5341 acgacgttct ctcaagagca attcaagcgt atcgccgcta tcctgcaacg aaagaccaat
     5401 catctcgatg tgcatatgac catatgcacg gcgacgcagc agcgtcagca ggcagcgata
     5461 gaactggcag gccatgtgga cgccatgatc gtcattggcg gcaagaacag cgccaacaca
     5521 ggtcgcctcg cgcaggtatg ccgtgaacag gattgcccga cataccacat cgaaacagca
     5581 gccgaattgg atacggcctg gttccgtggt atgaatcata taggcattac agccggcgct
     5641 tccacgccgg attggataat acaggaggtt gttaagatta tggaaaattt gcaagctgaa
     5701 ggcacagaag taatgagtga agaattgttg gacaagtatg actatgaaga aaatccaaag
     5761 aaaggtgacg tggtcaaagg cacggttatt tctgtgaatg atgatgctgc ctatgtttcc
     5821 atcggtacga aagcagaagc aatcctgccg aaaaaggaaa tggctgttnn nnnnnnnnat
     5881 ggctgttccc gctccggaaa aagcaagtga ctttgtcaaa gtcggcgacg aactgactgt
     5941 tgaaatcgcc aacaacatca aagaagaagg agctatcgtc gtttccctcg tcaagatgaa
     6001 aaaagtcgaa gactggaaag aagtccgcga tgcttttgaa aacgaccagc tcgtcgaatg
     6061 tacaggtaaa gaaacgaaca aggctggcct tgtcgtttcc atcaaatccc tgcgtggttt
     6121 cattcccctt tcccagggcg atatcaaatt cgttaaatct ttggactatc tcgtaggcca
     6181 gaccttccag gtcaaagtca tcgacatcga cgaacacaag aaccgcctcg tcctttcccg
     6241 caaagccgtc ctcgaagcag aacgcgaagc aaaacgcgaa gaagccctca aacacattga
     6301 agaaggcgct gtcctcgaag gcacggtcgt caagatcatg ccctatggtg cattcatcga
     6361 tctcggcggc gtagaaggcc tgctccacat ttccgacatt tcctggaaac gcatcagctc
     6421 tgtcgatgcc gttctccata cgggtgaaaa actccaggtc ctcgttcaga aattcgataa
     6481 agaacggaac cgtatttcct tgtccttgaa agcactccag aagaacccct ggatcgctgc
     6541 tatcgaaaaa tttgaagtcg gcgatatcgt caaaggcgaa gtcaagaagc tcctgccctt
     6601 tggcgctatc ctggccatcg atccggaatt gcagggcttg ctccacattt ccgaattgac
     6661 ggaaaaacgc ggcgttgctg tcaaagacct ggtcaacatc ggcgatgtca tgaacgtcaa
     6721 gatcatcggc atcgatacgg ataagaaaaa gatttctctc agcgtcttgg ccattcagaa
     6781 agatgaagaa gaacaggaag ttcgcaacta tctcgataac caggataaag aagaagaaaa
     6841 caactaaggt ttcacaaaaa ggggatagcc gggctctacc ggttatcctt ttttcgtcta
     6901 tagaaaggat tctgccatgt acaaaccctt agtcgctgtc gtcgggcgtc cgaacgtcgg
     6961 caaatcgacg ctcttcaatg ccattgtcaa gaagcgcatt tccatcgtcg aagacattcc
     7021 cggcgtgacg cgcgaccgca tttatttcga cgccgaatgg ctcggccagg aattcaccat
     7081 gatcgatacg ggcggcatcg aattcgtcga ttcagataac catatcttta ccagtatgcg
     7141 ttatcaggct gaactggcca tccgcgaagc cgacgtcatc ctctatgtcg tcgacggcaa
     7201 agtcggcgtc cagcctcagg atgaagaaat cgcccacctc ctccgctcat gcggcaagcc
     7261 ggtcatcctg gtcgtcaaca agatcgacag cgtcgaacaa gaaatgaata tctacgaatt
     7321 ttacagcctc ggcctgggcg accccatcgg cgtctcggct gtcaacctga tgaacctcgg
     7381 cgacctcctc gacgaagtcc tgaagtacat caagaaagtc ccggccatgg acgatgacga
     7441 cgaagccatc cacatcgccc tcgtcggccg gccgaatgtc ggcaagtcat ccctgaccaa
     7501 tgccctcctg gggcaggaac gggtcatcgt cagcaacgtg ccggggacga cgcgcgattc
     7561 catcgatacc cactggtcct atgaagggac ggatttcgtc ctcatcgata cggccggtat
     7621 gcgccgcaag gccaagatcg acatgcctgt cgaacgctat agcgtcgtcc gggccctgcg
     7681 cgccgtcgac cgttcggata tcgccgtcct ggtcatcgat gccacagaag gcgtgaccga
     7741 acaggatacg aagatcgccg gttacgtcca tgaagccggc aagggctgca tcatcgtcgt
     7801 caacaagtgg gacctcgtcg aaaaggacag caagacgagc cacaaatttg aagaagacat
     7861 ccgccgcgaa ctggctttcc tccagtatgc gcccatcctc ttcgcttcgg ccctgaccaa
     7921 gcagcgcatc aaccgcctgg ccgacatggt caagttcgtg tcggaacagc agcacatgcg
     7981 cgtgtcgacg agcgtcctca acgaactcat cgaagatgcc cagctgacca atccgccgcc
     8041 ggccaagggc ggcaagctgc tcaagattta ctacatgacc caggcttcgg tccagccgcc
     8101 gacgttcatc ctctttgtca acgaaccgca gctcatgcat ttctcgtacc tgcgctttct
     8161 ggaaaaccgc ctgcgcgaaa ccttcggctt tgaagggacg ccgatccggc tcatcctgcg
     8221 cgggaagaag gacggtgaaa gcttatgatg ctcggaatcc tctgcctggt actggcctat
     8281 atcgccgggt ccttcccaag cggtctggtc atcggcaagg ccttgtatca tacggatatc
     8341 agggactacg gcagccataa cacgggggcg accaacgcct accgcgtcct cggcgccgtc
     8401 ggcggcctgc tggtcctcat ctgcgatgtc gccaagggga tgctcggcgt ctacctcggc
     8461 caggtcgcca gccagtcggg cctgggcagt acggacacgc agatctactt catgatcgcc
     8521 ggcggcctgc tggccatcgt cggccattcg tgctcgattt tcctcaagtt caaaggcggc
     8581 aaaggcgtcg ccacgggcct gggcatcatc ctgttcctgg ccccgtggga aacgctcatc
     8641 gtcttctgcg tctgggccgt catcgtcgcc ctgacgcgca tcgtctccct cggttccatc
     8701 gtagcggcca tcctggtgcc cgttaccatg ttctttttca acgaacctct gccgctgacg
     8761 attttcggcc tgctggcagc cctgctggtc gtcgtgcgcc ataaagacaa cattatccgc
     8821 ctcatgcacg gcaaggaact gaaggtcgaa cgaattaaga aaaaatagat tgatatgtaa
     8881 taaaaaacgt gtataattaa ctatgtacga gcaggtgatc ctatgagaat catatccggc
     8941 agtgccagag gacgggtcct gaagtccccg aaggggatgc tgacccggcc gacgctggac
     9001 cgtacgcggg aaagtctctt caacatcctc gaaaacagcg gcggcctgcg cggtgccgct
     9061 gtcctcgata tctttgccgg tacgggtgcc ctcggtctgg aagcgctgag ccgcggtgcg
     9121 gcatcggctg tcttcatcga ccattacacg cagcagctca tccgccagaa tgcagccctt
     9181 tgcggctttg cagactgcgt cgaagtgctg cgccttaact cggccaaagc cctggcacgg
     9241 ctgcagggac gcaaatttga ttatatcttt gcggacccgc cgtataacaa aaatttggtg
     9301 aacgatacaa tcgctttgat aggtgaatac gatttgctgg ctccgggagg attgattctg
     9361 atggaataca gccggaacga aggtataacg gagagcccat tgtatgaggt catcagagaa
     9421 aaatcctatg ggaaagacac gcggattgcc tttatccgcc gtcgtcagga aggagtctag
     9481 aaagtgcgaa tcggtatttg tcccggcagt tttgatcctg tcaccaacgg acacattgac
     9541 atttttgaac gggggagcca gcttgtcgac aagctcatcg tcgctgtctt tgagaacccg
     9601 tcaaagaagc ccttgttctc gatggcagaa cgggaagaga tgttgcggga aacgacaaaa
     9661 cacattccca acatcgaagt cgtcagcttc catggcctgc tcaacgaata tgccatcgaa
     9721 cacggtgcga ctatcatcat ccgcggcctg cgcgccctga gcgnnnnnnn nnnacgaatt
     9781 ccagcgggcc ctgctcatga agaaggtgga accgcgtatc gaaacggtct tcatcatgac
     9841 cagtacccag tattcgtata tcagctcgac aggtatccgc gaactggcct gcttcggcgg
     9901 caagctcgac gatatggttc ccgattatgt cgaacagaaa ctgaaagaga agtttgcaga
     9961 caagtacaat gcttaggagt ggagaataca tgaattcgga taaactgtta gaagatttgg
    10021 aaagtttggt aatgaacgcc tcgaagatgc cttttaccaa taagaagatg gtcgaagaag
    10081 aagaaatcct tcagatcatc gacgatttga aagaatccat gccggccgaa ttggaacagg
    10141 ccaagaaagt cttgtccgaa aaagataaga tcatttccga tgcccagcat catgccgaaa
    10201 gcatggtcgc ccaggccaag gattatattg ccaaattgac agaagaaagc gaactggtac
    10261 ggcaggctca ggaacatgcc aaccagatca tccagaatgc caacgattcg tcggaacagc
    10321 tccattacct atgccggcga tgtcctcaaa tacgttgaat cgacattgga aaagaccttg
    10381 tccagcatcc agcagaaccg cgaaagcctg ctcaagaata acggacatca ggacgacaag
    10441 ccggatcagc agtaatcatg gcttatacga gagggactgt cgcatgaaag ttatcatgcg
    10501 aggtcccttt ttttatccgc atggcgggta gataggaggt actatgaacg aaattacgtc
    10561 ccgcgacaac cagtgggtca agatggcctg ctccctgaag cagaagaaag gccgccaggc
    10621 ccatcagcgc gtcttcgtcg aaggcctgcg ggtcattgcc gacgcagcgg ccatggctat
    10681 tcccgatgcc atctgcttcg tctcggaaaa ggggcgggcc ctggatggct ttgaagccct
    10741 ctatgccaga ggcctcgaat tgggatggac tttttatgcc gtcaccgaca gcgtctatga
    10801 caagctgaaa gacaccagga cgccccaggg attggcggcc atgctgcctt tctttgccta
    10861 tactttcgac agcctgccgg ccattgcgcc ggaaaaggcc gtcctctatc tgcgctccat
    10921 ccaggacccg ggcaatctgg ggaccatcct gcgcacggcg gctgcggcca atgccggcgc
    10981 cgtcctgctc agcgaaggca gtgtcgacgt ctataatgac aagaccatcc gcagtgccat
    11041 gggggctatc ttcaaggtcc ccgtcgtcca ggacgtgacc gatgaacagc tggaccagtt
    11101 ctgtgccgac gaggaccgcc aggtctatgg gacggctccg gaaggggccg tctcgtatgt
    11161 cgacgccgac tacagccagc ctgtcatcct ggccttcggc aacgagggca acggcctgcc
    11221 tggtcctttc ctggactgct gcgatgacgt actgaccatc cccatgcgtc ccgatacgga
    11281 atcgctgaac ctgtccatgt cggtcggcat cgtcttatat aaggcatggg aaaaaaatgg
    11341 ttttaaggag taactatgga agaaaataag gaaaccctcg atttgggctt tgccaaactg
    11401 gacttgcagc gtcagcagcg ctgcggcttc ccggaagtcg tctactgtgc cggcaagact
    11461 gtcaatcagt cggtacggat cctggacatc cttatggaga agtacgacaa cgtcatcggc
    11521 acgcgggccg ataaggccgt ctacgacgcc ctggccggac gccatccggc tgcccagtat
    11581 gacgaattgg cccgtatggt ctatcagcat aaggataaaa ccgtcgtcaa tggcgaccgc
    11641 ctcatttcgg tcattaccgc cgggacgagc gatatcccca ttgccgaaga agcggccttg
    11701 acggcggaaa tcatgggcaa ccgcgttgaa cgcatctacg acgtcggcgt cgccggcatc
    11761 caccgcctcc tggcccgcgt cgatgatatc cgccgggcca acgtaaatat cgtcgtcgcc
    11821 ggcatggaag gggctctggc cagcgtcgtc ggcggcctcg tcgacaaacc ggtcatcgct
    11881 gtgccgacca gtatcggata tggtgccaat ttccacggcc tgtcggccct cctggcgatg
    11941 ctcaacagct gtgctgccgg cgtatccgtc gtcaatatcg acaacggctt cggcgctggc
    12001 cgcctggccg atacgatgaa tcgcttgagg tgatggaaat ggaaacatta tggcagctgg
    12061 aagccaatat cgatgacatg aacccgcaga acatggaata cgtcttttcc ctcatcctgg
    12121 ctcagggcgc caacgatgtc tgggccatgc cgatgatgat gaagaaaggc cgcatggcct
    12181 ctatgctctg cgtcttgtgc cgtcaggaac tcatcgacac cctcatcggc gtcattgtca
    12241 gggagacgac gtccattggc gtccgcttct tcccagtgac ccgcctggcc tgcgaccggc
    12301 tcatcacgac ggtccgcgtc gatggccgcg acctgcactg caaggtctgc cgctatgacg
    12361 gcaaggtcgt caacatatcg gcagaatacg atgattgccg ggctgccgcc gctgagacag
    12421 ggacgcccct gaaggattgg cagcgcaaag tgaaggaagc ggcatatcgg caatttgcgg
    12481 aatagaatgg gcagaggata ttgtcaaaat acgattgtcc atgtataata gtcattaagc
    12541 tcattatgta gtacgtcctg aaaggacggg gagtttgaaa gatgaacaga gtcatcatca
    12601 atgccgatga tttcggcatc catacagaag tcaatcaggc cgtcatcgaa gcctgcgacc
    12661 aaggtgtcct gaccagcacg tctctgctgg ccaacggcct ttgacgaggc cgtcgacctg
    12721 gcccggaaat gtcccggcct gggcatcggc atccatctca gcctggtcgg ttctctgccg
    12781 acggtcctgt cggccaggga agtgccgacc ctggtccagc ccgacggcct gctgccggaa
    12841 agctatacgg aagtcatcaa gcgggcctgt cagggaaaac tcgattacgg tcaggtctac
    12901 cgggaactcg acgcccagat ggaaaaaatc atggctacgg gccttcccat cgaccacctg
    12961 gacagccacc agcacctgca cgtcctgccg caggtctggt ccatcgtcca gtcgctgatg
    13021 atgaagtacg gtatccaccg cctgcgcatc cccagggaag cctattccta taaggtattg
    13081 tcggccaatc ccgtgcgcat tgccggccgc gacggcctga cctacttgtc gaagaaagcc
    13141 atggcctcgg tccggcgcct gcactttacg acgacggact ttttctgggg catggtcgac
    13201 ggcgggcaca tgacggaaag caatctgcac tatctcatcg acagactgcc ttttgggacc
    13261 catgaaatca tgatgcatcc aggccgcagt acggctgtcc tcagccagtc ctttgcctgg
    13321 ggctatcact ggcaggatga attccaggcc ctgctgtcac cggctatccg ccagcagctg
    13381 gaagccaaac agattgaact cattacttac ggtgacctgc cgtaaggatt ggaggtctta
    13441 ctttgcatat tttagttacc ggcggggccg gcttcatcgg ttcccacctc gtcgacgcct
    13501 tgctggaaga cggccatgac gtgaccgtct ttgataatct gagtacaggc tgccgggacc
    13561 agattccgac agaggcccgt ttcgtcgagg gcgatatccg cgacgaagca gccctgcagg
    13621 ccctctttga agaaggccat ttcgacgtcg tctttcatga agcggcccag acccaggtat
    13681 cctactccct ggcccatccc agggaagatg ccgaactcaa cgtcatcggc ctgattaatg
    13741 tcctggaaca gtgccgccgt cagggtgtac agaaattcat ctattcgtcg tcggccgccg
    13801 tctatggcga taatcctaaa gtgcccctga gcgaatcgga actactggca ccggcgtcgt
    13861 tctacggctt gaccaaagtg gctgcagaaa agtatatcca gctctatggc agcctgttcg
    13921 gcctgcccta tgccatcctg cgctatgcca atgtatatgg cgaacgccag ggcaatggcg
    13981 gcgaaggcgg tgtcgtcggc ctctttgcca gagcattggc ctgcggtcag gacctgacga
    14041 ttttcggcga cggtgaacag tcccgcgact tcgtctacgt caaggatgtg gcccgcgcca
    14101 atatcgccgc tatggacagc ggcgtgccgt cagggattta taacatcagc acccagatcg
    14161 agacgaccat caacgccctg aaagagattc tcttgtattt ctctcatgcc ggtgtggccg
    14221 tttcttatgg agaagcccgc aagggcgata ttttccggtc gtccctggcc aatggcaagg
    14281 ccatcgaaac gctccgctgg cggccgaaga tgaaactcat gccgggcctc atggcgacgt
    14341 atcaatattt tctcgaaagg gaggaagccg agtgaaagaa gacagcaacc ataaatttta
    14401 cctggtccgc gaagacattc tgcccgaagc catcaagaag accatcaagg tcaaggaaat
    14461 tctcaagcat catcagacgc gcaccatcaa cgaagccgtc cgcatgatgg acttgagccg
    14521 cagtgcgtac tataagtata aggactatgt cttcccgttt tacgatgtga ctcaggggaa
    14581 aatcgtcacc ttgtcggtca tgatcttcga ccggcctggg gaattatcgc aagtgctcaa
    14641 cacggtcgct gccaataacg gcagtatcct gaccatcaac caggggattc ccttgcaggg
    14701 aatggcagat gtcagcttgt ccatcgaaac caaggatatg accgttcctg tcgatgaact
    14761 gattaaaacg ttggaaaagc tgtccggtgt cagcaaagtc gatatcatcg gacaagcgta
    14821 agataaaaaa gggggagtag gctcatgaaa aatgtttcca ttgccttgtt aggctgcggt
    14881 acggtcggaa aaggggttgt cgacttactg gccatgaacg gtccgctcat tgaagaacag
    14941 ctcaatacga aaatcaacat caagcgggtc ctcatccgcg atttgaataa gtacgaaaag
    15001 atggaagact tacctgattc catccagctc acgacggatt tcaacgatat catcaacgac
    15061 gatgaaatcg acattgtcgt cgaagtcatg ggcagcgccg attttgccaa ggactgcatc
    15121 gaacagtgct tcctgcgcgg caagagcgtc atcagtgcca ataaggacct catcgccgat
    15181 tacggcattc cgctattgaa attatcgcag cagcacaacg tggacttcca gttcgaagcc
    15241 agtgtcgccg gcggcatccc catcgtgcgc ccgatgtatt cgtcgctcaa cagcaaccgc
    15301 atcgaagaaa tcatcggcat catgaacggc acgacgaact tcatcttgtc caacatgacc
    15361 gcctatggct cgtcgtatga agacgtcctc aaggaagccc aggaaaaggg ctatgccgaa
    15421 gcggacccga cgaacgacgt cagcggttac gatgcggccc gcaagatcgc catcctggca
    15481 acgctgggct tccactcggc tgtgaccttc aacgacgtcc atgtcgaagg cattgaaaac
    15541 attagcaaag aagatattga acacgccacg gaaatgggct atgtcatcaa gctcctggcc
    15601 attgcacgcc aggatgaaga cggcatttcc ctcaacgtcc atccggcctt catccgcaaa
    15661 ggccacccgc tggccaatgt cgacggcgct tacaatgccg tctgcgtccg cggcaactgc
    15721 gtcggcgacg tcatgttcta cggccagggc gccggctcct tgccgacagc cagtgccgtt
    15781 gtcagcgacg tcatgaacgt catcctccac cgcaacctga agatgacggg caaacgggat
    15841 tacgtatggc acgaagccaa gctcaaaccg caggccgaca tttcctcgcc gtactacatc
    15901 cgcatgatcg tcaacaatgc gccgggcgtc ctggcggctg tttccaaggt actggcagaa
    15961 aacaatatca gtatccgctc cctcatccag aaggatgaaa cggcagaaat cgctgaaatc
    16021 gtcatcctca tcgacaaatc gccagcaggc ctggtccagg cttcgctcca gaaaatcgaa
    16081 gacttgtcgt gcgttcgcaa gattgcgaat gccattcgtg taatcgaggt gtaagaaatg
    16141 acaaaaacgc tgcacgcaca gattccggcc acgtcggcta acgtcggctc cggctttgat
    16201 gccgtcggga ttgcgctgac tttgtacaat gacatttatt ttacggaaga accggataaa
    16261 gacacgatca ctgtcgaaat cgaaggtttg ggagccaatt ccctgccccg taatttcgat
    16321 gagaatatgg tcggacaggc catgaaggcc gtggccatca agaaccgcca gtccctgccc
    16381 aagggcggca cactgcggct ggtcaatgct attccgccgg cccgcggcct gggaagcagt
    16441 tcggcagccc tcgtcggcgg catcctcatc ggcaatgcct tgacgggcaa taagatgacc
    16501 cgcgaagata tgctgaccat ggcgaccgtc ctggaaggcc atcctgacaa cgtggctcct
    16561 gccatttacg gcggcctgtc cgtttctatc atggtcgatg gcaagaccct gaccaattcc
    16621 atccccatcg acgatgacct gtctttcatt acggtcagcc cggaagtcga agtttcgacg
    16681 gaagaagccc gcaaggccct acccaagacc attgactacc agagcgccgt cttcaacgtc
    16741 agccgcgtca gcttcctcgt ttcttcgctg ttcaccaagc agtacgaccg catcgcctat
    16801 ggcctgcagg ataaactgca cgtgccctac cgcctgcgcc tcatccccag cggcgaactg
    16861 gtcctcaaga aggccgtcga agccggtgcc ctgggcgcta ccatcagcgg ttccggttcg
    16921 acgctcatcg cctttgcgac ggatcaggaa gtgcagatca tggacaccat gatcaactgc
    16981 ttcaacgacc acggcatcgc cgctacgggc catatcctga aatgcagcaa tgaaggagcg
    17041 aaactcgtcg aatgatacaa gacattggca atcacacgta ccatgtcgaa taccaccctg
    17101 tgcctctggc gcgggatggt attctgctgt cttatcaggg ccgcaaggtc ctggtccggc
    17161 tgacgggcga taaagaattc gtctatcccc gccatgccga ctatgccgac atcggagaag
    17221 accagttccg ctggctcttt gccgaagacc gggcccagtt ctacctggcg aaggaagccc
    17281 tgccggaacg ggacggcttt acctatgtgt ccatcaattt catgcgcgct gcccggccgc
    17341 gttacctggc ctttgccggc atcgccggcc tgtccctgca caactggtac agcaaccaca
    17401 tctattgtgg ccattgcggc cgccccatgg tacacagcga gaacgaacgc atggtacact
    17461 gcgaccactg ccacaccatg atttatccgc gcatctgtcc ggccgtcatc gtcgccgtcc
    17521 gccatggcga caaactgctg gtctcgaagt atgccggacg ggaatacaag aatatcgcct
    17581 tgctggccgg ttttgccgaa gtcggtgaga ccatcgaaga aacggtccac cgggaagtca
    17641 tggaagaagt gggcatccgt gtcaagaatc tgcagttcta taagagccag ccctggtgct
    17701 tcacggatac gctgctcttt ggcttcttct gcgacctcga cggcgacgat acgctgaaag
    17761 tcgaccacgg cgaattggcc acggccggct ggatcgaccg caaggacatt ccggaagata
    17821 cggaaaaggc cagcctgacc atggaaatga tgaccctctt caagcagggc gtctattgat
    17881 tttatgcgga gtaccttgac aggcccaagt aaacttgcta caatatttat cacataaaag
    17941 cgatgagcgg aagaagcctt atttcttttt gtcagcgaga tgccgagtcc ggtgcgaggg
    18001 catccaaaga gggtagggcg cgtcacccgg gagctggcag gaggaaaggc ctgcccgtgc
    18061 agcagacgtt acctgtgcca tgaagcggtc gcgcaagcgg caaattaggt ggtaccgcgg
    18121 aagatacagc attctttcgt cctaaggaat acccttaggc gagggaatgc tcttttttgt
    18181 tttggaggga atatagatga aatggaataa ggaacagtta ggcgcttatg caccgaacga
    18241 cattgaaatg tcctggtatg atttttggga aaagaatggc tacttccatc aggacccgga
    18301 tccgagcaaa gaaccgttca gcatcgtcat gccgccgccg aacgtcacgg gtcagctcca
    18361 catgggccac gccctggaca ataccctgca ggatatcctc gtccgcttca aacgcatgga
    18421 aggctataac gtcgcctgga tccccggcac ggaccacgct ggcattgcaa cccaggtcaa
    18481 agtcgaacag cagctggcca aagaagaagg caagtcccgc tacgacatcg gccgtgaaga
    18541 attcctgaaa cgcgtctggg cctggaagga acagtacggc agccgcatcg aaaaacaggt
    18601 ccgccgcctc ggatcttcct gcgactggag ccgcaagcgc ttcaccatgg acgatacctg
    18661 cgcccgcgcc gtccgcgaag tcttcgtaac gctctatgaa aaaggcctca tctaccaggg
    18721 ccagcgcatc accaactggt gcccgcactg ccatacggcc ctgtcggata tcgaagtcga
    18781 ccattccgac gtccagggcc atctctggta catcaaatac cctgtcgtcg gcgaagatga
    18841 ctacatcatg atcgctacga cccgtccgga aacgatcatg ggcgatacgg ctgtcgccgt
    18901 caacccggct gacgaccgca tgaagaaata catcggcaag aaagtcgtcg ttcctctcat
    18961 cgaccgcgaa gtcgaagtca tcgccgacga ttacgtcgat atcggcttcg gtacgggcgc
    19021 cgtcaagatt acgccggctc acgacccgaa cgactttgaa atgggccagc gccacaacct
    19081 ggaatccatc atgatcatga acctcgacgg cacgatgaat gaaaaagccg gtgccaaata
    19141 caacggcatg acccgggaag actgccggaa agccgtcgtc gccgacttga aagaattggg
    19201 cctcctggac cacatcgaag aactgaccca tgccgtcggc cattgctcgc gctgcaagac
    19261 gacagtcgaa ccgttcgtca ccaagcagtg gttcgtcaag atgaaaccct tgactgaagc
    19321 cgccctcaag gccgtcgaag acggcaagac gaagttcatc cccgaccgtt tcgtcaagac
    19381 ctataatcac tggctggaag acgtccacga ctggtgcatc agtcgtcagc tctggtgggg
    19441 ccatcagatc cctgtctggt actgcgatga ctgcggcaag tcttccgtca gccgggaaga
    19501 catcacagaa tgtcagcact gccacagcaa acatatccat cgcgacccgg acgtcctcga
    19561 tacatggttc agttcggctc tctggccgtt ctcgactatg ggctggcccg ataagacgcc
    19621 ggatatccag cagttcttcc cgaccagcgt cctggtcacg ggctatgaca tcattttctt
    19681 ctgggtcgcc cgcatgatgt tcatgacctg cgaattcatg aaggaaatcc ccttcaagaa
    19741 cgtcttcatc cacggcctgg tccgcgacag ccagggccgc aagatgagta aatccctggg
    19801 caacggcatc gatcccctcg gcgtctgcga acagtacggc gccgatgccc tgcgtttcac
    19861 cctggtcacg ggcaatacgc cgggcaacga catgcgcttc tacatggaac gcgtcgaagc
    19921 gaaccgtaac tttgccaata agatctggaa cgcttctaaa ttcgtcatca tgaacctcgg
    19981 tgatttcgac gaaaccttca tccctgaaga taaagacctg accctggccg accgctggat
    20041 cctcacatcc ttcaatgaaa ccgtcgccaa agtcacggaa gatctggata aatttgaact
    20101 cggcgacgct gccgatgccg tttacaactt catctggaac tcctactgcg actggtacat
    20161 cgaactggcc aagaaacgcc tttaccaggc cgaaagcgaa cgggacaagc acacggttca
    20221 gtacgtcctg gtctatgtcc tgacgcacac cctggaaatg ctccatccct tcatgccctt
    20281 cgttacggaa cacctctggc agcacctgcc tcacgaaggc gaaagcatca tcgtcgctcc
    20341 ctggccgaag acgcaggata aatggaactt cccggccgat gccgctacga tgaataccat
    20401 gatggaagcc atcaagggta tccgtaacct ccgcgccgaa tcgaacgtcc ccatgggcaa
    20461 gaaggcgccg gtcatcctgg ctccggctac ggaagacatg gctcagacct tgaagactta
    20521 cgaagattac ttccataccc tggcctttgc cgataaagta accctcctcg gtgcgggcga
    20581 tgccaaaccg gaaaatgccg tcgtcgccgt tgtcccgggc attgaagtct acttacagct
    20641 caaagacctc atcgacgtcg aaaaagaaaa actccagaag gaaatcgccc gcctcgacaa
    20701 gaagctgtcc aaccagggct tcctctcgaa agcaccggca gcggtcgtcg aaaaagaaaa
    20761 gggcaagctc gccgactacc aggaaaagat ggctgctttg accaagcgca tggaagacct
    20821 ggctaaactc taagaggcag ctatgaccta cgaacaagca atcgactatc tggaatcagc
    20881 caatgtcttt ggcatccagc tggggctcgg ccgtatccag gccttactgg aacggctgga
    20941 caatccccag aacaactata agaccatcca cgtcaccggg accaacggca agggctcggt
    21001 gacggctatg atcgcctcgg tcctgactga agcgggcttc cggacgggcc gctacacgtc
    21061 gcctcatctg gaagactata cggaacggat ccatatcgac aaccatgaca tttcccgcga
    21121 cgactttgcc aaagccgtat ccgtcgtcaa agacgccgtg acggccatgg tcgctgccgg
    21181 cggggaaggg ccgacggaat ttgaaatgat tacggctgct gctttctggt acttcgacta
    21241 tatcggcgtc gattacgccg tcatcgaagt gggcttgggc ggcctcctcg attcgacgaa
    21301 cgtcatcgtc cccgtcgtct cggtcatcac taacgtcgcc atggaccaca tgaaatactg
    21361 tggtaatacg atcgaagcca ttgccgaaca gaaagcgggc atcatcaagg aaggcgtacc
    21421 tgtcgtcacg accgccgatg ggccggcttt gcaggtcatc gcccgcaagg cctatgaaaa
    21481 gcacagccgc ctctatgctc tggaccacag cttcgacgtc gtcggcaacg ccggaccgga
    21541 agacccggaa gccggtcaga tggtgacggt ccgggaaaaa tacggctttg ccattacgac
    21601 ggtcctgccc ctgctgggca aacaccagcg gacgaattgc gccgctgccg tcatgacact
    21661 gctcatcttg gcccgccatg aaaagaaact gacccggacg gtcctggaaa acggcatccg
    21721 ccatgtccag tggccaggcc gcttccaggt cttcaaagcc ggtgacatcg acgtcgtcct
    21781 cgacggcgcc cacaacccgg ctggcatcga caccttctgc aagacttacg aagacgtctt
    21841 cgcctcgcgg aagcgggtct tcctcttctc cgtcctggcc gacaaagatt acagtcagat
    21901 ggtacgggaa ctcttccacg acgacgatta cgtcgtctgc gcaccggcac cgacgccgcg
    21961 ctcagccgac cccaaggcca tggcggccat cctgccgtgc aaagccgatt gggccggcac
    22021 catcgccgaa ggcctggaca aagccctggc tgccatcaaa ccggaccagg tcctctgcat
    22081 cgtcggctct ctgtacatcc agggagaagt ccggaaatac ttccggaaga gataccatat
    22141 tgagtaaggg ctgagcggct agcagcttgt aggggccgtc ccaaagggcg gcccgcttga
    22201 ttcctcttta tagcagctag cggtgagcca ctagccattg aaaaggcgct gtcttacagn
    22261 nnnnnnnnng tcttacagga cagcgccttt tcgttgcgcc tctgcgcctt tttatggtaa
    22321 aatgatacat atattttaca cggaatgtaa ggagcgaaca ttatgaagtg gatgatacgg
    22381 accggcagta cgccagccga gcgggcacgg ctgcgcgtcg tctatgctat ggctggtact
    22441 gtctttttta tgccggtgaa cttgttcatt atggaaggct ttttcatcat cgccctgggc
    22501 ctggggctct attactgtaa aaaatatccc aaactgcccc tgcgccagtc gccgttgtcc
    22561 ctgccggctg tcggctttgc cctggtggcc tttctgtctc tcgtcggctc gccgcacttt
    22621 ctcttaggga cggctttcta tgccttcacg gttttgcagt acctcgtgct ctactttggc
    22681 atcctcctgt tcgtccgcca ttcctgggaa cggcgcctgc tcttttcgac gctcctcctc
    22741 agtgccttcg tcgtcgccct gtacggcctg taccagtatg cccacatgct gaccctccac
    22801 gaggccgaat gggtcgacaa ctcagccttt cccatgctgc ggcgccgcat gtactcgacg
    22861 ctgtacaacc ctaacttatt gtcggccttc ctgctgatta tcatgagcgc tgcggcgtcg
    22921 atgatgatct gtacccgcca tcgctggcat cacgtcatgt acctggcctt ttttgccatc
    22981 ctgaccctct gcctgatcct gacctattcc cgtggcgcat ggctcagcgt ctgcgctttg
    23041 atcttctttt tcggcctctt ttgggataaa cgggtgtggc tcctctttct ggcaggcccg
    23101 ctgatcctgg ctttctacca cggcggcgtc gccgaccgcc tgatgtccat tttcagccac
    23161 agcgaagcgg acacgtccgt ttccatgcgc atggacatgt gggaggcagc cattgccatg
    23221 tttgtcgacc atcccgtcct gggcattggc tggggcgcct tcaaacacgt ctatcccgtc
    23281 tataatgaat tgatccagga agcaggtatc gtcatcttcc acgcccataa tatgtacctc
    23341 aacatcctgg cagaaacggg catggccggc ctgggcttcg gcttatggtt ctttttcggc
    23401 aatgcctggt atgccatccg ctatgtacgc agccatgcgg cccacacctt cagccgatcc
    23461 ctggccatga ccctggccgc tgccgtcttg tccctgggaa tcagcggcat gagcgactac
    23521 gatttattta gtacccagat ttcacttact ttttggctga tgagtgccct ttttgctaac
    23581 atgtatggcg aagaatgtga aaaaaaacga taaaaatagt ttgcgaaata attcacaatg
    23641 atatataata agtctgtgac tattatcact agataagatg acttatagct atagggaata
    23701 gcttgggaca tagttggagg gtattatcca tgagacagaa aaaggcaata tcggatgctg
    23761 tcatcgaacg attgccgctg tactacaggg acttgctatt attgcaagac gcgggaatcg
    23821 atatcatctc atccgaaaag ttaggggaac ggttggggat tacgccggaa caaatccgca
    23881 aagacctgac ctgctttggg gcttttggaa aaaagggcgt aggctattac gtcagtgaat
    23941 tatgccagca gatcgtggaa atcatcggcc tgcagcagca ttggaaaatc ggtatcgccg
    24001 gcatcggcca tctcggctgg gccttggcga actacaagac tttcggacgc ttgggcttcc
    24061 gcacgacagg cttgttcgac gtcgatcacc gcatcatcgg tcagaacgtc ggcggcgtcg
    24121 aagtcacgcc gatttctgaa atgggcgaag tcattcagcg cgaaggcatc cagatcggta
    24181 tcatcacgac accggcagct gtagctcagc aggtcgccga ccagatggtc gccgccggca
    24241 tccggggcat cttgtccttt gcaccgctgc gcctgaccgt accggacggc atcttcgtct
    24301 tccgcgaaga cctgtccatc ggcttgagcc gcatttcgta ctatttgacg aataagaagg
    24361 attgatgatg gccgtccgct attggcatgg cgggcgccct tacattatct ttatagtttg
    24421 tagtacagat agtttttggg gtgtaggggt cgccacgtgc gagcccctac agcaaaaagg
    24481 cgctgtcttc atgcgacagc gccttttgtg tgctataata ttcgtacatt cccgataatt
    24541 tatagaatga ggtgatacta ctatggattc tatctatggt tcctatggac cggctttgca
    24601 ggctgctctg gaagatatta tcggccgtat ccgcgcctat agtgacgata taaaggccaa
    24661 gaccggggaa gcggcttatg aacacctgat ttaccgcatc aagtccgaag ggagtatgcg
    24721 ggaaaaatgt gaccgcaagg gcctgcctca gacggcctat tcggctctcc acgatatccg
    24781 cgatgccatc ggcatccgta tcgtctgttg tttcctcgac gacatctatc gggctatcga
    24841 cttcctgaaa agcctcgatg gcgtccgcgt cgtcgaagag aaagattata tccgccacgt
    24901 caaaccgaac ggctaccgca gttaccacgt cattttggaa ctggaagagc cttatgaaga
    24961 tgtgaccggc gccaatccgg gtcatttcta tgccgaagtc cagctgcgca cgattgccat
    25021 ggattcctgg gccagcctgg aacatcagat gaaatacaag cacgacatca agaacccgga
    25081 actcatcgtc cgggaactca agcgctgtgc cgatgaactg gcttcgtgcg acgtgtccat
    25141 gcagactatc cgcaatttga tccatgcaca agaataaggg ggaaacgcta tgcgccttct
    25201 attagctgaa gatgaaaaag aaatgtccgc agctctgtct gccatcctga cccattcggg
    25261 atacgatgtc gatgccgtct atgacggcca ggctgccgtc gaaaaagccc tgtcccagtc
    25321 ctatgactgt atgatcttcg acatcatgat gccgaagaag gacggcgtcc aggccttgaa
    25381 ggaaatccgc gacaaaggga atatgacgcc ggtcatcatg ctgacggcca aagccgaaat
    25441 cgacgaccgc attaccggcc tcgacgccgg tgccgacgac tacttgacca agcccttcgc
    25501 catgggcgaa ctcctggccc gcctgcgctc cctgacgcgg cggtcgacgt cctatgcgcc
    25561 gtcgaaactc actgtcggca atgtcgaact cgacgtggaa gaacaggaac tggcctgccg
    25621 caattctatc cgcctggcca ataaggaaac gaagctcatg aaattcttca tggccaacgc
    25681 cggcaaaacc ctgtcgacgg cccagatttt tgataacgtc tggggcgatg aagaagacga
    25741 cgtcgatgaa ggcatcgtct tcctttacgt ctcgtattta cgggaaaagc tcgaagccgt
    25801 ccaggccgac attgaaatcg tcggccaaag agggcaggac tatgccttga ccgtgaaaga
    25861 agcgtctgcg gagggttaga ccatgaatat gatcaagcgc ctgcgccgta aattcatcat
    25921 catggcgaca atcggcgttc tcgtcgtcct cggcggcgcc ctgggcctca tcaataccat
    25981 cgcctatatg cggatgaatc agcaggtcat gtcggtcctg acctatatca tccataacaa
    26041 cggcgctgtg ccccagaaaa tcggcggcga cgatggcagt ttcttcagcg acccgtcatg
    26101 gaccgaagag acgccagaat ttgcctacca gatacgcttt ttcagcgtcc tcgtcaatgc
    26161 cggcggctat gcggaaaaaa tcgacatcca gcacatcgct tcgttttctg aaaaagaagc
    26221 cattgaatac gcccggacga ctgtcgctga ggcgcgggaa aacggaacga aacagggctt
    26281 tttcaaaaag aaccgggcca gttatgccta tatgattcag cccaacgacg acggcagctt
    26341 cctcatcgtc atcctggact gcacgcgcga cgtcgctgct gtcacttcgt tcatgcgcta
    26401 ttctctctgg tttggcctgg gctgtgtcat cttatacgtc atcatcctgg cggccatgtg
    26461 caacgtcgct atccggcctt tcgtccgcaa catggagaac cagaagcgct tcatcaccaa
    26521 tgccgggcac gaactcaaga cgccgattgc catcatctcc gccaatgccg aagccatcga
    26581 gatgctcaat ggccagagcc agtggacgga cagcatcctc aagcaggtcc gccgcctgtc
    26641 caacctcatc cagaacctca tcatgctgtc caagatgggc gaacgctctc aggtcgacct
    26701 ggtcattacc gatgtgaact tgtcggaaac ggtccatgcc gtagccgact ccttccagca
    26761 gctggccatt gacgccgaaa agaccttgca tacggatatt gccgacggca tccacgtcaa
    26821 aggcgacagt aagtgcctct atgaactggt caacatcctc gtcgacaacg ctgtcaagta
    26881 ttgcgatgac aaggggacca tcgacgtctc cctgcgccgt ccccgtctgg ggaagggggc
    26941 gattgtcgcc gtgaccaaca cctatgccga cggggctaat gtggattatt cccgcttctt
    27001 tgaacgcttc taccgcggcg atacgtccca taacagccag aaggccggct atggcatcgg
    27061 cttgtccatg gccgaagaat tgacgaaact catgggcggt aagattaatg tatcctataa
    27121 ggcccaacgt ataacgttta ctgttcggct gggctgattt tccctatagg gaaagcgcct
    27181 gacctgtgtt ataatgtgac caacatcact tttattcagg gggaagatac caatggaaat
    27241 gcacatggaa tatatccgaa aattaccgga accgcaggaa ttgatggaac aatttcccat
    27301 cgatgacaag gtcaaggcca tcaaggcgaa acgggatgaa gaaatcaaaa acatcctgac
    27361 cggccaagac gaccgcttcc tgctcatcat cggtccctgt tcggccgaca atgaaccggc
    27421 tgtcctcgac tatatccatc gcctggtcaa ggtccaggaa caggtcaaag ataaaatctt
    27481 tatcatcccg cgcgtctaca ccagcaaacc ccgtaccatc ggcgtcggct ataagggcat
    27541 gcttcatcag ccggacccgg aaggcaagga agacatgctg ggcggcatca tcgccatccg
    27601 tgaaatgaat gcccgcgtcg tccgcgaaac gggctttacc tgcgctgaag aagtcctcta
    27661 tccggaaatc ttccgttact tgtcggacct cttgtcctat gccgccgtcg gcgcccggtc
    27721 cgtcgaagac cagttccacc gcatgattgc cagtggcgtc ggcatccctg tgggcatgaa
    27781 gaacccgacg tccggtgaca tttcggtcat gctcaactcc atcgaagcgg cccagcatac
    27841 gcaggatttc ctcttccgcg gctgggaagt ccgcaccaaa gggaatccgc tggcccacgc
    27901 catcctgcgc ggctatgtcg acagcttcgg caacagcatg cctaactacc attatgaaaa
    27961 cctccagcgc ctgtacgaag cctatggcaa gcgggacctg tcctatccgg ccgtcatcgt
    28021 cgacgccaac catgccaatt cgggcaagaa atacctcgaa cagatacgca tcgccaaaga
    28081 cgtcatccac agccgcaagc actcggccga catcaaacag ctggtcaagg gcttgatgat
    28141 cgaaagctac atcgaagacg gcaatcagaa aatcagcgaa ggcacctatg gcaaatccat
    28201 caccgacccc tgcctgggct gggaaaaatc cgaacgcctc atctacgata tggcagaatt
    28261 gctgtaatta gtggctcgtg gctagtggtt cgtttttgcg gtcttatagc cgcactgtag
    28321 gagcttctct atagtttgta gtacggagtt tgtagtttgt agaagagggc cttaaattaa
    28381 gaaccaacc
//
LOCUS       DAWABZ010000010        28236 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_485_length_28236_cov_3.738192, whole genome shotgun sequence.
ACCESSION   DAWABZ010000010 DAWABZ010000000
VERSION     DAWABZ010000010.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 28236)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 28236)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..28236
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_485_length_28236_cov_3.738192"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    12617..12626
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13598..13607
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6192 a         7838 c         7561 g         6625 t
ORIGIN      
        1 cagcgtcgtt gtcagtttga gtctgtcgcg gcgtagccgc atctgagtcc gaggaaaggc
       61 cttaaatttt agaaatggaa atccagttca gtccggaaga gattacggtc atattcatcc
      121 gacgtactcc agtggcgttt ctgtttggaa tacatcgttt cccagataat gtttttccac
      181 gggacataac ggacaccggc aatccaaccc ttggtgccat tccaaatgga gttccattcg
      241 tcatccccag cgagccagcc gttttcgcca tatttaatat aacgcagata aaggccataa
      301 ctgccaggct ttttataatc agcatctttg taattcaaac gcaaagcgat gctgttgttc
      361 tggaaatcgg catcggtttt ggcatagtcg gcaataaagc gcaagtcttt attgatgttc
      421 gtagccatac tgaggacata gccataactg cgtttattcg tcgtatcctg attatcgtat
      481 actggcgata cgcggccata atttttattg ggagtcccat cgtctttaaa ttccttcatg
      541 tccgtctggt agtacgaaac attgccagaa ccgtacttct catttttacc taagctgctc
      601 tttgcatagg caaagttgat atccaggttc ttggccacag acccccaaac agcagcaccg
      661 tataagcttt ccctgttccc tgcacctgtc gaagcgaacc agaaaccact gccagtcaat
      721 ttcgttcccg gaatgggcgc tccgaattgg ataccgtcag tttcattacc gacgaggaca
      781 ttctgaaagc ctaagccgcg ccaggcacga ccaatgctga caaaacgacc gtctttctgc
      841 ccttcgaccc aaattcgctg catattgccg tcatggccgg accaagtctg ttccccattc
      901 gagttaaagg cattttcact gcgccgatga ccgtcattat aatggcggtt cgtttcgctt
      961 tggaacttga ccgtccagta atcgttgacc ttaaagtcgc cgcgcaagtc catgtaaaaa
     1021 cgattggtct gtttatcaat agtgccggag gtcgtatctt tgtcataatc gtactgtgta
     1081 cgcatgaagc catacaattt gactcggtcg attttttcct tgttttcctg gatatccttg
     1141 gtattctgct cgatctgctt ggtgttcttg gcgacttgct gctgcaagac gttgatttcg
     1201 tcggcatatt cggcgtgcag tttttctagg accgtcttgt caccgaagtt gctgccgcct
     1261 ttttgcatgg ctttggcgac gatgctggcg acttcgtagc gggtcatggt gcggccgcct
     1321 tcaaaggtgc tgttgcccat gccttcgatg acgccttctt ttgccaggta atccagggct
     1381 tcataggacc agtggtcttt ggggacatcg gagaagctgt cagccgatgc ggccagggcc
     1441 gtgccggcag tgccgagagt cacggcagcc aggacaacca gggctttttt attcaaatcc
     1501 atggtaagga cctcttttct tataaattat cttccagcat ggcccctacg ctgtcataga
     1561 ctttacgagc ctggtcctgg atagccggac gggccgtggc gacggtgtag ccgttatagc
     1621 ccaggatcat gggcaggtcg ttgatttcat cgccaatagc caggatgtcc gcaccgtccc
     1681 agccaaagag ggagagcgtg tgggcaattg cctgttgttt actgatatcg gagggaatga
     1741 tgtcgacggc acagcggttg ggataggcat gaacggtatc gccgaaatgg gcgttgacct
     1801 gttcgctcgc tgccagggca tcgtcgggat cggtgaagcc cagggaatac tggtgaatcg
     1861 tctggggcag atggtcgatg tcgtcttccg ttatcggcac gatggggtag tcccattctt
     1921 tcgcttcccg ccagacccag gtgcccggca aatcatggca gagataggtc cggtcctgcg
     1981 aagagaagga gaaatggaag gacttgcgga ccagctccag cttgctgacg gccttgagga
     2041 cggccggttc gatgcgggct tcccagagga cgcggtcctg gtcatcgctg atgaggccgc
     2101 cgttattgca ggccaggtaa tcatattcga cgccatagtg tttgagctgg ggcatgagca
     2161 tgccgtggtc ccgtccggac acgacgccga atttatggcc tgcagcccgc catctgcgga
     2221 tggcttcggc atcgcgggcc gtaatgatat cgtcgcggaa cagggttccg tcataatcgc
     2281 tggcagcaat ttttaacata ataatcctcc tcatacacga tagatgacgg cttcggccgg
     2341 gcgcaatctt ccggcttcct gggcgtcgta gttgcccaga aggaccgttg cgccttccgt
     2401 cagcgatgcg tcataatcga tggtttcgtt ggtaaaattg accagcgtca atgtcgcctg
     2461 gtcgtctaag atacgcttga aagcgtaaat gcgttcatca tcagccagca gctcttcgta
     2521 gcgccctcgc tgcaggatgg ctgccgcttc cccctggaag cgttcggcct ggatgcggcg
     2581 gtagtaccag aggaccgaat cagcatcgtg ttcttcgctc tcagcacagc actgcaggta
     2641 atcgtcatgg acgggcagcc acggcgtccc cgtcgtaaag tcggcctggg ctgccgtcgt
     2701 ccactgcatg ggcgtccggg cattatctcg gctgtgggcc tggacgaggt gcagggcctc
     2761 tgccggtgca aagccttcat ccagggccag ctgatactgg ccgtgcgtcg aaatgtcatt
     2821 gtagtcttcg atacgggcaa aggccgtatt ggccatgcct aattcctggc cttcgtaaat
     2881 gaagggcgtc ccgcgcaggc tgaacaagat ggtcgtcatg gctttggccg ccttcttctt
     2941 atccgtccct tcggggaaat aacggttgac acagcgtatc tggtcgtggt tctcgaagaa
     3001 gatgggatac cagccattgg ctgccgtcgc ctgctggctg tcggacaggg ccttcttgag
     3061 cttggtcagg ggccattttt cagcgcggca ccagacttca tcgtcaggaa attccaggtt
     3121 cacatggctg aactcgaaga gcatgctgaa agccccgtcc ttaccgaccc actggggcag
     3181 gtcagccggc gtgacgccgt tggcttcggc gacggtgaag atatcgtggc cgtcgaagac
     3241 cttccggcgg aattcgtgga gaaagtcgag gatgcccggc gtattagcga tcatcttgtg
     3301 gatggacgac aggccgtcgg cgccgtcgac ggggccgtct gtgaagacgg ccggtttctt
     3361 gatgtagacg atggcatcga tgcggaagcc gccgacgcct ttggctatcc agaaattggc
     3421 cgcatcatag agggcctggc ggacggccgg attttcccaa ttcaaatcgg gctgttcgac
     3481 ggcgaaggtg tgcaggtaat actgctgccg ttcttcgcac cacgtccagg ccgggccgcc
     3541 gaagatggag cgccagttcg tcggcgccga gccgtcttct ttggcatcgc gccagatgta
     3601 ccagtccgct ttgtcgttcg tgcggctgcg catcgattcc tggaaccagg cgtgctggtc
     3661 cgacgtgtgg ttgtacacca ggtccatgac gacgcggata tcccgcttct tcccttcggc
     3721 aatgagggta tccatatctt ctatcgtgcc gtagcgcccg tcgatggccg tataatcgct
     3781 gatatcgtag ccgttgtcga ccatgggcga cgggtagacc ggcgtcagcc agatggcccc
     3841 gacaccgagc ttcttgagat aatccaagtg gcgcgtaacg cctgcaatat cgccttcgcc
     3901 gctgcccgtc gtgtcctgga agctgcgggg ataaatctga tagacagccg tctgctgcca
     3961 ccatttcaaa gtatccataa aaccctccta gaaaatgaca atcggtgttt caccagcgac
     4021 gacaggctgg tccggttcct tcttcatctg cgggtattcc gtcgagttgg tgacggccat
     4081 gatgacgatg tcgttatagc cggctttggc gatggcatcg cggtcgaagc ggatcaaggg
     4141 gtcaccggct ttgacctggg cgtcctgctg ggtcagcagc gtgaacccgt cgccattcat
     4201 attgactgta tccaagccga tgtggatgag cacttcggca ccgttgctca gttccaggcc
     4261 aatcgcatgg agcgagcttt ccatgatcat ggaaacggtc gcgtctgccg gggcgacgat
     4321 ggtatcgccc gacggtttga tagcgacgcc atcgcccatc atcttctgcg agaaggcatc
     4381 gtccggtact tcgctgatgt cgacgaggtc gccggagaca aaggctttca gcttcatgga
     4441 acggtcgtct tcatcgacgg aagcgatgga ttcagcagcc ggagctgccg gcgtttctgc
     4501 cgaggcttct ttcgtttccg gaaccgtgtt ttctacggct gccggcattt cccctttcat
     4561 gagggcgtcg aagtggccgc ggacattggg cacggacagg ccgacgatga cctggatggc
     4621 gtggccgttc ttgaccaggc caaaggcacc ggcattggtg aacttgctgg tcgcagcgac
     4681 cttatccggg tcgttgaccg tgacgcgcag gcgggttgcg cagttggtga cttcgcggat
     4741 gttatccgga ccgccgaggc aatcgaggaa gacctgggct ttcagggcat cggcaccgcc
     4801 ggcaatgcct ttcttttcca tttctttctt ggctttgtat tccgctttgg tgaacaattt
     4861 gtcttcgccg ccatcatcgg tacggcccgg cgtgggcaaa tcgaagtgaa ggatgaggaa
     4921 gcggaagacg acgaagtaaa tggccgtaaa gccgaggcca atggcaatct gcaaaatgta
     4981 ggtccaggca tgatagcgga acaaggggat ccagttctgg acgaagcagt cgatgaggcc
     5041 gccgccgaag ttgcccgaca ggccgatgaa atagagcgtc gccgacaagg tagccgctaa
     5101 gacggcatgg acgacgtaca agagcggagc gacgaacaag aaggtaaatt caatcggttc
     5161 ggtaatgccg cagaggacgg ctgtaatcgt agccggaatg agcagggttg cgactttctt
     5221 gcgtttttcc ggtttggcac aatagtacat ggccagggct gcgccgggca ggccgaagac
     5281 tttggcactg ccgtggaggg caaagccgcc ttgggggaac atgtcccgca aggaatgagc
     5341 actggtggcg aaatcattga ggtgctgcag ccagtaagcc tggatgccgc cgtcgcagac
     5401 agccggaccg taaatgaagg gcaggtaaat aaaatgatgc aggcctgccg gcaagagaat
     5461 gcgttccgag aaggtgtaag cccagacgcc gaagatgccg ctggtcttga ggaagaactg
     5521 gaactgttcg ataccgtgct ggataggcgg ccagatgtaa cagaagagga gcgccatggg
     5581 cagcatgacc aggaagccga cggcgacgac gaaggccggt cccttgaaga tgccgagcca
     5641 gtccgggatt tccgtatcat agaaatggtt gtgcaggtag gccgagatgc aggcaatgaa
     5701 gatagcgccg agcatgccca tgtccagggt cttgatgttg gcaatcatgg ccaggcccgt
     5761 gccggcaccg gcgttctgac tatagtcgac gccgaagtag ctgccgtgaa gggtcaggat
     5821 accggcgatg aaatagttaa ataccatgta gatgacgaaa ccttccatgg cacagcgggc
     5881 ctgttctttc ttggcaaagc caatgggaac ggcgatggca aagaggatcg gcatctgatt
     5941 gaagacggtc caggcgccct gctggacgac gtaccagaaa tcataccaga gggtcccttt
     6001 ttctgccagg ctgccgaaga ggcccgtatt gctgaagatg atcgacaagg cgacagtcag
     6061 cccgaagaag gcgaataaga tgaccggtgt gtacatagcg ccgccaaaac gctgcagttc
     6121 ctgcatcacg cggtccttgt tgaatgacat agacaattcc tcccgtttgt gaaacaaagt
     6181 acataagctt tccttagtaa caggatataa aaaaaacgcc gcgaatacaa tagattcgcg
     6241 gcgttctggc acacggcccc cggcctgtaa cctggtacat ttcctgtact ttggtacagc
     6301 ctatcgggaa gcctcttttt cttgtttcaa atggtgcaga tacatacaat atttcacata
     6361 ccatatctca gccaggacaa aataaggcat catggactga aaggcgacgt ggtcgtcttc
     6421 cttgtcatag agctggaagc gggccggcga gacgtaaaaa ttgaccgtcg acagcgacgc
     6481 cagggtattg tcgtgcaggc gggtaatgga aatgaaggga atgtcccgca gtttcagggc
     6541 ctgggccagt tccttaacgg ccggcgattc gccggagaag gagacgataa tgaacaagtc
     6601 gtctttggtc atggtccgga tgaggttgcg gaactcttcc cgtgccggta cgtcgtagag
     6661 catgacgtta tcgtagaaaa aaagacgctt cagctcctgg acgacgttat tctggacgta
     6721 gcccgacgca taggtgaaaa tccggttggc ttcatggacg agggcgctgg cgctatcgaa
     6781 gttacggctg cacagcttgt ccacggtctg ttcatagaag cgctggagtt cgtccatgac
     6841 attcgtatca tagcaggaaa tttccggttc ttcccgcttg aggagcatct tgaattcacc
     6901 gaagccgtcg aggcctaatt tctgggcaaa gcggacaatc gtcgtcgtcg agacgtgaca
     6961 ggccttggcc agttcatgga tcgagctgtg gcggacagcg gcgcggtgct gcaagatgta
     7021 cttccatatc cccatatccg tattattgag ctgctgacga tgggcgttga tgagttcggc
     7081 aagttgcatg gctggttccc ccttcacgaa acaggccccg gcggaaaaat ccgcaggggc
     7141 cgggtcatag tttcattata aggaatccag ggacggacgt caagctggtg ggcgctgtgc
     7201 cgcacagaaa aaggctgcca ttttactaca aactacaaac taaaaactcc ataagaagct
     7261 gtctgtatag aatagcactt ccgctggctg actattatct acaaagtgcc gcagcgatgg
     7321 cagcgccgac ggaggcgccg ttgctgttgg ggatgacgtg gatgcgcgta ttttcggtcg
     7381 gagccatgca gaagacgcag atggcatctt cgatgagcga cggaacgcct ttgatctttt
     7441 cgaccaggct gccttcgatg gcgatggact gttccgtaaa gcccttatcc tctgccaagt
     7501 gactgagaag gccgaagctg gccgccccga tgagctgggc tgcccgggca aagacggccc
     7561 ggaccagggc acagaggcct tcttgctctt cttcgctgac ggcgaaaccg agcagttctt
     7621 ctgccgtctg ctggccggaa atgagttcgt tcatgtcgcg cgtcgtaaaa tacgggatgt
     7681 cccgtttgag gtattcgcca aagacgacgc ggcagacttc gctcaaatag cggccgccga
     7741 tcatcttttc aaagggatgg tcgccaggca gctgagacgc ttcatcgacg aggcggtccc
     7801 agaccgtagc cgggccgccg tcgaagccgg ccgcttccag gttgacgatc atctgcaggg
     7861 ccggttcata atagcacatg ttaaagcccg tgccacagac gatgccgatg cgcgacgggt
     7921 tgtgctgata gaccgaagaa aggagggtcg ccgtcgtatc gttgagcagg gccaccggtt
     7981 cgatgcggtc caggccggcc cggacaaggg cctctttcag caggccattg atggactggc
     8041 cggccacgcc gggaacggcg atttccttgg accaggcgat gaggctggca tccttggccg
     8101 acgtctggtc gacggcaaag gaaaaggtaa agcccagttt atagtcgtga ttgccgtcag
     8161 ccgcttcctt gacgatggcg gcgatgaagt cgaagagttc gtctgccgtc ttcgacgaag
     8221 ccgtgtagtc gtagtcagcg catttcaagg gtttggccac cttgctttct acggcaaagc
     8281 cctgttcgcc catgaggcgg atgcgcgaag cccggacgtt cgtgccgccg aaatccaggg
     8341 ccaggaaggt ccccgtttcc cgtcccgtcg gcaagcccag ataggtgcgc agcgccaaaa
     8401 gggagctctc cttcccttcc agagcgtctt cggcactctt ccggaagtgc tgtgccagtt
     8461 cccgcagctc tgcgccggat aactgcaatg cctgtctcat tttttcccaa ttttttatcg
     8521 tatctgccat gatcgttggc ctcctgtctg tacgcaaaaa catctatatt ctcatgataa
     8581 tgaaaaaagg ccataaagac aactctttta tggcctttcg gttccgtaac acgacaatgg
     8641 acggtagtac aaaatatgga tattggtaca ggcgctgcgc gtaaaaataa aaaggaaccg
     8701 tgcacgatgg tgaagacctt catgctccag ttccttgaat agagtttggt gggcgataac
     8761 aggatcgaac tgctgacatc ctgcttgtaa ggcaggcgct ctcccagctg agctaatcgc
     8821 ccaagtggtg acccctacgg gatttgaacc catgttctcg ccgtgaaagg gcgatgtctt
     8881 agaccgcttg accaaggggc cacgtatgtg gaatgtggtg atccacccga gattcgaact
     8941 cgggacaccc tgattaaaag tcaggtgctc tgccaactga gctagtggat cataatggca
     9001 ggggtagtag gattcgaacc tacggatgac ggagtcagag tccgttgcct taccgcttgg
     9061 cgacacccct gtatggctcc tcaagtagga ctcgaaccta cgacaaatcg gttaacagcc
     9121 gattgctcta ccaactgagc tattgaggaa taaatggagc ggaaaacgag actcgaactc
     9181 gcgaccccca ccttggcaag gtgatgctct accactgagc tacttccgca tgacgacttt
     9241 ctaagtatat cacatcggga ttgatatgtc aacgctgtat tccggcgggc cgttgtgccc
     9301 gtcacagtgc ttaattataa taccatgacg tatgagattt gtcaagaaaa aaataggctg
     9361 tcgcattaag gcagcctatt tttgagtatt aagtttgcag tttgtagttt ttagtgaatg
     9421 gttttaaatt taaagccatt ttctgcaaac tacaaactct gtactacaaa ctataaagag
     9481 aaaggagcct tgcatgatgg caaatccatt cgtgtgacaa tcccctacga tttacagcct
     9541 acgatatttc ccctgcctgt ccgatgaagc cgcggacgac gttggggata gtcaggtctt
     9601 tcgttccagc tccgtaggcc acggtgtcgt agacgaaatc cttcgggcgg acgccggcat
     9661 gcgccaggac tttgaccagg gcagcgccgg tcggcgtgac cagttcgcct ttgatatccg
     9721 tcgcatacca gggaatcccg gccaggagtt cggccgtcgc cggtgccggg acgggcatga
     9781 tgccgtgagc gcagcggaca aagccgctgc cggcatggag cggcgacgtg gcgatttgtt
     9841 cgatgcccag gtatttcagg caccagaccg taccgacgat atccaggata cagtcgatgg
     9901 cgccgacttc atgaaagtgt atctcgtcga cggggacgcc atgcaccttg gcttcggctt
     9961 ctgccagggc ctggaatacg gccaggctct tggccttgat atcgtcctcg ataggtgcac
    10021 cttcgatgat cttggtaatt tccggaaggc cccggtgctg atggtgatga tgatggcccg
    10081 tcatatcgac gagatggccc tggccggcca actcttcccc atgatgcgcg tggtgttcat
    10141 ggtgttcgtg tccctgttca tggtcgtgat gctcatgatg gtcgtgagcc ccgtggtcat
    10201 gacgttctgc atcgaggagg acattataat agatagactg gatgcccagc ttgttgacgc
    10261 tgtggcacac caagtcatag cccgtcaggt tcagcgaggc catggcctgt ttaaaggctt
    10321 caaaggggac accggcctgg atgagcatgc cgatgaacat attgccgctg ataccggaaa
    10381 agcaatcgag gtataaggtt ttcatggaaa tcgctcctta ttttaaattt tcattcatca
    10441 tctgtaccga tgcgtcgagc atggtcaggc ccatagccga gcccatgccc tggtcgaggt
    10501 tctggtccag ttcgacgaag ggcttgaggc ccagtttctt catctgcatc tgatggaccg
    10561 gttccagata gcgcgccgaa gcgcagacgt aatcgtcaat cgtcgggcat aaggcgcggg
    10621 caatgaggac ggcagagccg gtgacggcgt tgtcaaagat gacgagcagc ttgcgctggg
    10681 ctgccgacag gatgaagccg accatggcgg caatgtccgg agccccggca cggcgcagga
    10741 ggtccagggg atctgacgca tggaagtggt gctggtcgat gacttccttc aatttctcta
    10801 atttctgacc ggacttcatg tgctccggca gttcttccag ctggtcccgg aaaaagacag
    10861 ctgtcaatat gaaagccgtc agcagtcccc gttcgccgag gttccccaga cctacagccg
    10921 tatagccttc atcggccagg ttctgtccga tctgcaggcc gctgaagagg gcatccatca
    10981 tttcatcttc gctcatggca tcctggcggg cccagaaatg gctgccgtgc atgactttta
    11041 aattctgtac gccctgcgat tccgggatat cttcttccag gcccatgtca aggagcatga
    11101 cgccggcacc gatgcggtgg gccaccttat tgataggccc tcggccagtg gcgatttcca
    11161 gggcgtcggc cttgctgttc ttgcccttgg tcttattttc tccgccatcg acggcatggt
    11221 cagctgcaaa gacgacgacg gctttctgca atttttccgg atgcagcgtc ccctgtacac
    11281 cggcaatgcg gcctgccaga taggacaact tgctgtcctg gcgtatggtc agactggcca
    11341 ggcgctgata gctctctgcc gtttttgcca aatccatcgg ttcgatggca ctgatgactt
    11401 gttctaatac ttggtccata atcccctccg tggcgtgaaa agtttgaaaa ggagctgtcg
    11461 caataagaca gctccttttt gagttttaag tttgaagttt tcagtttgta gtatatggta
    11521 aaaatttaaa aacatcttct acaaactaca aactacaaac tccatactac aaactaatct
    11581 tattataaaa ttcattatac acgaaaaagc cagttatcgc tacatcatga cgttcatcgt
    11641 agtccgttca tcgttcatcg aagcacttca accaacggcc ttcaaggtat tataacgaac
    11701 ggccgaattc gtcggtatcc atgatggctt ttttgaaagc ttctttggtc agcggttcgg
    11761 ggacgcaggt ccattcggtc agggccggag cagcatcaac gatggaatcg acttcgccgg
    11821 ctttgatatc gaggtcagcc agggtcgtcg ggtaatggca cttcttatag agggccgcta
    11881 cctggtcgcg cagttcaaac tgtttgtcat aggtcagcag gaccaggacg ccgaaggaaa
    11941 ccgtttcgcc atggagatgg ccttcgacga cttgcggcag gaccgtcgac gcgttataga
    12001 acatgtgagc cagggacgaa ttgtagtagt agtcgccgcc gcccgtcgta cagttggaaa
    12061 cgaggcccgt gctgatgatg atatccagga cgacctgctg cagggcatag gtgatcttct
    12121 tggccttgaa atcttccagg gcctgttcac catactgcaa gagcggcgcc gtgcaggctt
    12181 tgccgatggc ccggcccagg aggaccgtat ggaagtgttc cgccgtacgg ctggccaatt
    12241 ccgattcaca ttctttactc atagcatcgc cgatgccggc ccagaagaac ttgaaaggcg
    12301 agtcggcaat gatggccata ttgatgaagg tatggaccgg cggctggtcg agatagtagt
    12361 agccggcaaa ggatttatcg gctttgtaca tgacagccag ggccgtcgat ggagcacagt
    12421 tggaagccag cgtcgggaaa gcgaacaagg ccttgccagc ccggtcagcg acgactttac
    12481 aggtatcgat ggcacggccg ccgccgacgg cgaagaccag gtcagcatcc tggacggcct
    12541 gttcacggag caggcggtcg acgtgttcat acgtcgcatc gtcgccgtac cagagtacgc
    12601 cgaggacttc caggtcnnnn nnnnnnttcc aggtccgtct tggcgatggc atcgagcaag
    12661 ggctgttttg ccttggacag ggccgtcttg ccgccgatga tgacgacttt tttgccgaaa
    12721 tgacgggtaa tcttgccgat agcatcataa gcatggacgc caatggtaaa agccggcaga
    12781 accaaagtat agtcagtttg agacatgata aaaacctcct gtaataatga aaaaagataa
    12841 gttcaaaagc tatagccatt atagcattgt ccactggccg tggctagggt aaaaggttta
    12901 acttttctat tcgcaatacc tatgaaaaat cgctatgttg acatttatcc atatgacttt
    12961 cagtaattat atggcttatc tagtatgtaa attaaacgtt cattgctata tatagtatga
    13021 aaacgtttac tataacaaat tgtaatttga taatgtattt tgagttataa tcagttagcg
    13081 tactatgctg agtgtacatc ttgcaattcg ggaaaactgc tctataataa gggagcgtaa
    13141 tgtagtgtac cccatttcca agcgttgtac gttggtatca ttttgtttca tacggaaagg
    13201 gttgcacgca tactatttta tcaaaggagg ttctttttgt atgaacggtt tgtatctcgt
    13261 tattatttca gcattagtgt tcgtactggc ttaccgtttt tacggcgctt tcatcgccgc
    13321 aaaagtcttg acgctgaacc agtacaaagt cacaccggca ttccgctttg aagacggtca
    13381 tgactacgta ccgaccaaca agtacgtcgt cttcggccat cacttcgcag ccatcgctgg
    13441 tgcgggcccg cttgtcggcc cggtcatcgc cgcccagttc ggttatctgc ccggcgcttt
    13501 atggattctc atcggtgggg ttctcgccgg tgctgtccat gacatggtca tcctcttctg
    13561 ctccatgcgt tacgatggca agtccatcgc cgaaatcnnn nnnnnnntgg ccagaagaca
    13621 ggcgtagcta cttctttcgc cgtcctgttc ctgaatatcc tctgcatggc tggcatggcc
    13681 gtcgttatcg ccaatgccct ggcaaacagc ccgtggggta ctttcaccat cggcatgacc
    13741 attcctatcg ctatcttcat cggcatctac atgcagttcc tgcgtcctgg caagatcaaa
    13801 gaaggcacca tcatcggcgt catcctcacc ttgctggctg ttgtcgctgg tccggcagtc
    13861 caggcttcgc cggtcttagg gcccatgttc accatcagcc ataacggcat ctgcatcgcc
    13921 ctgatcatct acggcttcat cgccgcagcc cttccggtat ggctccttct ggcaccgcgt
    13981 gattacctct ctacgtacat gaaaatcggt actatcggtg ccctggctct cggcatcctc
    14041 atcgtaggcc cgcagattca gatgccggca acgacgcagt tctgcgctgg cggcggcccg
    14101 gtcattaccg gtgcagtcct tccgtacatc ttcgttacca tcgcctgcgg tgctgtttct
    14161 ggtttccatt ccatgatttc caccggcacg acgccgaaga tgctcatgaa cgaacgctcc
    14221 atcctgcctg tcggctatgg tgctatggtt actgaatcct tcgtcgctat gatggctctg
    14281 attgcagcct gcgccctcgt accgaacgat tacttcgcta tcaactcttc ggctgcacac
    14341 ttccaggctc tgggcatcca ggtcgtcgac ctgccgcacc tgaacgaaat ggtcggtgaa
    14401 aacgtcgctc atcgtccggg cggtgctgtt tccctggctg tcggcatggc ttacatcttc
    14461 tcgaacctgc ctggcctcga ccacctcatg aactactggt atcattgctg catcatgttt
    14521 gaagctttgt tcatcatgac tatcatcgat gctggtactc gtgtcggccg ttacatgctc
    14581 caggaactcg tcggccacgt atggccgaaa ttcggtgacc cgaactggaa accgggtgct
    14641 atcgtagcat cggctttgat ctgcgcagca tggggttacc tcgtactgaa cggcaacttg
    14701 tccacgattt ggccgatttt cggcgtatcc aaccagctgt tggctatcat cgccctctcc
    14761 atttcgtcgg ttgtcatctg ctccatgggc aaagcccgtt acctctgggt tactggtctt
    14821 ccctgggctt tcctgatggt catgattttc tgggccgact tcctgaacat cttcgaaatc
    14881 tacctgccga agggtgaatg gacgatgttc acagtctcca tcatcatggc cgtcctggtc
    14941 atcatcgtag ctatcggtgc tatccgtaga tgcatctacc tggctaagac cgtaccggcc
    15001 agctatgata cgacagaaac tgtcgaagca gaagaactca aacatctcca ggaactcgtt
    15061 aagacggata aagccgctca gcgtttccaa gaattaacca ttgctcatca ctaagagcga
    15121 gtaaaaaagg cttgtcgcac atgcgacaag ccttttttct gtaggggcga ccacgtgggg
    15181 cgcccgttta cgcaaatcgg ttcatggata ttctacatat ccccagcggg ctcgccacgt
    15241 gcgagcccct acaggcacga atcacgagcc actgtcaata atgcacaaag tgcattgttg
    15301 accatcgata atatactcaa aattctcttt gcgcaaagga aatgcccgtt ttataacatt
    15361 ttattatttt tattatgaat ttatatcatg ctgttttcgt aatctttact ataagtaact
    15421 tgaatttttc tctcctttag cctatagtaa gtgtataagt acagtgtata ctttatttct
    15481 cgtgcgatac ataattcata ttacaccaat tagaaataag tatagctccg gatccgtggg
    15541 aagtagagta gcctacacac aatttcataa aggagggatt gttttatgaa tggtttgtat
    15601 ttagtcatta tctctgcatt agtatttgta ctggcttacc gtttctatgg cgctttcatc
    15661 gctgccaaag tcttgacggt caaccagtac aaggtcacgc cggccttccg ctttgaagac
    15721 ggccacgact acgtgcctac caacaagtac gtcgtcttcg gccatcactt cgccgctatc
    15781 gccggtgccg gcccgctcgt aggcccggtc attgccgctc agttcggtta tctgcccggt
    15841 gccttgtgga tcctcatcgg tggcgtcctc gccggtgccg tccacgacat ggtcatcctc
    15901 ttctgctcca tgcgttacga tggcaaatcc atcgctgaga tcgcaaaagc ccagattggt
    15961 gacaagactg gcctggctac gtccttcgcc gtcctgttcc tcaacatcct ggctatggcc
    16021 ggcatggccg tcgtcatcgt caacgccctg cacgacagcc cgtggggtac gttcaccatc
    16081 ggcatgacca ttcctattgc tattttcatc ggtatttata tgcagttcct gcgccctggc
    16141 cgcattaaag aaggcaccat catcggcgtc gccctgacct tgctggccgt cgtcctcggc
    16201 ccggccgtcc aggcatcgcc taccttggct ccgctcttta ccatcagcgc caaaggcatt
    16261 tccatcgccc tcatcatcta cggcttcgta gccgctgccc ttccggtatg gctgctcctg
    16321 gcaccgcgtg attacctctc gacgtacatg aagatcggga ccatcggcgc cctggccatc
    16381 ggcatcatca tcgtcggccc gaccgtccag atgccggcta tcacccagtt ctgcgccggc
    16441 ggcggcccgg tcatcaccgg tccggtcctg ccgtacatct tcatcaccat cgcctgtggt
    16501 gctatctccg gcttccactg catgatttct acgggcacga cgccgaagat gctcatgaac
    16561 gaacgctcta tcctgcccgt cggctacggc gctatgctga ccgaatcctt cgtcggcatg
    16621 atggctttga ttgcagcctg ctcgctcgta ccgaatgact acttcgccat caactcgtcg
    16681 gccgaacatt tccaggctct gggcatccag gtcgtcgacc tgccccagct gaacgccatg
    16741 gtcggcgaaa acgtcgccca tcgtccgggc ggtgctgttt ccctggctgt cggcatggct
    16801 ttcatcttct ccaacatccc cggcctcgac cacctcatga attactggta tcacttctgc
    16861 atcatgtttg aagctctctt catcatgacc atcatcgatg ccggtacccg tgtcggccgt
    16921 tacatgcttc aggaactcat cggccgcgta tggccgaagt tcggcgatcc ccactggctg
    16981 cccggcgcaa tcatcgcttc ggctctgatc tccggtgcat ggggttacat cgtcctccaa
    17041 gggaacttgt ccactatctg gcctatcttc ggtgtatcca accagctgct ggccatcatc
    17101 accctggcca tctcgacggt tgtcatctgc gctatgggta aagcccgctt tgcctgggtc
    17161 acggttatcc cctgggtctt cctgaccgtc gtcatcttct gggccgatta cctcaacatg
    17221 ttcaacatct atctgcccaa acaagaatgg ctcctgttca ccgtttcggc catcatggct
    17281 ctcctggtca tcatcgtcag catctcctcc atcagaagat gcctgtacct ggctaagacc
    17341 gtaccgccca gctacgatac gacagaaact gtagaagccg aagaattgaa acacctggaa
    17401 gaactcatca agaccgaccc ggtcgccaaa cgcttcaaag aacttacagt tgatcatcat
    17461 tagaaaaaag aggcttgtcg cacagcggca agccttttta ggttgttcgt ggggcgtccg
    17521 ctcgcgcaaa ccgacctcgg tattcccaca tatatcgggc ggactcgcca cgtgcgagcc
    17581 cctacaatct ataaacgtct tacctaatca tttaacgttt aaccttgttt tattttaaaa
    17641 atttatggaa aaacatcttg acatagacca gcaaattgcg tacaatgtgt tcaagcttca
    17701 ggaaacaaac cgtgaagcga aatattcgta aatctaatga cgagagaaag tagtctgtac
    17761 cattgctttt cagagagctg gcggccggtg cgagccagta cgatgatacc gatgaagctc
    17821 ctctccgaag atcgacgctg aatttcatta ggcgccgacg gcatttcacc gttaccagaa
    17881 acgcgtatgt tggtacgttg tagagagtcc ggctgcagca atgcgccggg aatcagggtg
    17941 gtatcgcgga attatactcc gtcccttaca gaaggggcgg agtttttttg ttggcctctt
    18001 ttgacaaatc tctaaatccc aacgataaaa ttcttatttt atattacaaa ggagatatgt
    18061 tatcatggca aaagtatttt atgatcagga tgtaaactgg gatgtaatga aaggcaaaaa
    18121 agtcgcaatc atcggttatg gcagccaggg ccacgctcat gcgttgaacc tcaaagacag
    18181 cggcatcgac gtcgtcgtcg gcttgtatga aggcagcaaa tccatcgcca aagcccaggc
    18241 agccggcctt gaagttaaat ccgttcctga agccgttaaa gaagccgata tcacgatgat
    18301 cttgattccg gacgaaaaac aggctgacgt ctacgcaaaa gaaatcgctc ccaacttgaa
    18361 agacggcagc gccctcgctt tcgctcacgg tttcaacgtt cactttaaac agattgttcc
    18421 tccttcgaca gtcgacgtct tcatggtcgc tccgaaaggc ccgggccacc tcgtccgtcg
    18481 tactttcact gaaggcggcg gtgttcctga cgtattcgct gtcgaacagg acgctacagg
    18541 caaagccttc gacatcgctt tggcctatgc ccgcggcatc ggcggtaccc gtgccggcgt
    18601 catccagacg actttccagg aagaaacgga aacagacctc ttcggcgaac aggcagtcct
    18661 ctgcggcggc atctgccagt tgatcaccaa cggttttgaa accctttgcg aagctggtta
    18721 tcagccggaa atcgcttact ttgaaacctt ccatgaaatg aagttgatcg tcgacctcat
    18781 gtacgaaggc ggcatggcta agatgcgtaa atccatcagc gacacggcag aatacggcga
    18841 ctacacagcc ggcccgcgcg tcatcacgca ggattccaag aaagccatga aagctgtcct
    18901 cgacgacatc cagaccggtg ctttcgctaa agattggctc ctcgaaaaca aagccggcgg
    18961 ccgcgctcac ttcctcgcta tgcgccgtca gcacgctgaa catccgattg aaaaagtcgg
    19021 cgctaaactc cgcgacatga tgccgtggct ccacaacaac gaccagaacg actaagtaag
    19081 atccgttcat cgcaggccgt tcgtcgttca tcgaaaagga cgatgaatgg ccgcttcgat
    19141 gaacgaacta cgacaaacga tgaacgatat taagaggtga atgatgatga aaatgacagg
    19201 tgcaagggca gtgctggaat gtttgaaacg ggaaggcgtc gacgtcgtct tcggctatcc
    19261 cggaggggct atcctgccac tatacgaaga aatgtataaa gacaacttcc ggcacatcct
    19321 taccggtcat gaacaaggag cggtccacgc tgcagacggc tacgcccgcg ttaccggccg
    19381 gcccggcgtc tgcatcgcca cgtccggacc aggggtatgc aacatggtta cgggcattgc
    19441 tacggccaac atggactcta tccccatggt catcatcagc ggacaggtcc cgacgggcct
    19501 catcggccgc gattccttcc aggaagccga tatttccggc attacgtcgc ccattaccaa
    19561 gcacaactac ctggtaaaga acgccgcttc tatcccgcgc gtcctgaaag aagcctttta
    19621 catcgcatcg acaggccggc ccggtccagt cctgatcgat attgccaaag acgccctgga
    19681 agcggaaatc tatttccaat atcccgataa agtccatctg ccgggctacg tcgtcgaaac
    19741 aactggacaa gcttatgaca tcgaccgggc tgtcgccatg atccagcaca gccagaaacc
    19801 ggtcttcctc gtcggcggcg gtgtcatcct gtcggaaacg acgaagtatc tcgaagacat
    19861 cgtcaatacg acgaagattc ccgtcgtcac gacactcatg ggcaaaggag ccatccccga
    19921 ttctcacgaa ctccatctcg gtatgggcgg gatgcacggc tcttatgcat cgaacaaagc
    19981 catctcaggc tgcgatctgc tcatcgcctt aggggcccgt ttcgacgaac gcctgaccag
    20041 ccgagccgat acgtttgctc ccaatgcaca gatcatccat tttgaaatcg atgatgtcga
    20101 aatcgacaag atcatcgaca tcgacttagg catcaaaggg gacctgcgtt ggtccctgcc
    20161 cattttcgcc aagaagctga aagaaggcgc cttcgattat ggagccgcct ttgccgattg
    20221 gcgtcatgag gtcctggcca tgaagaaaag ccaccccttc gcgacgccgc cgcgtccagg
    20281 ctgcatttca ccccagtcaa tcttcgccgc cgtccgggac atcacccggg acgacgatac
    20341 cgtagccatt accgacgtcg gtcagcatca gatgtgggca gctcaattct tcacgacgga
    20401 tgagccgaaa cacttcattt cttccggtgg tctgggcacg atgggcttcg gcctgccggc
    20461 tgccctcggt gccaaagtgg cctgtcccga aaaaacagtc gtcgtctttt ccggcgacgg
    20521 cagtatcctc atgaactgtc aggaattcgc gaccttgtcg gaatacggca tcgacgtgaa
    20581 agtcatcgtc ttgcacaacg atacgctggg aatgatcgtc cagctccagg atttcttcta
    20641 taaccaccat tattcacaat gcactttgaa taagcgcaaa aacctgccca tgctggccga
    20701 atcgctgggc gtcaaaggat acgccatcga aaaacaggac gatttaaccc ccgtcctgaa
    20761 agaggccttt gcccataaag ggccggccct ggtcgatatc cgcatctcgc cggaagaacg
    20821 ggtatacccg acagttcccg gcggcaaagc attgagtgac atgattttag gaggtgacgc
    20881 ctgatgagaa aacagcatct ggctattttg tccgataata aaccgggcgt attgacacat
    20941 attgctggcc tgatcagccg caaggccatc aacatcgaat cgttgaccgt cggttatacg
    21001 gaagaaccag atgtgtcgcg cttcaatatc gtcatcagca tcgaagacga taaagaattg
    21061 aagcagacca tcagccagct ggccaagctc atcgacgtca tcaagatcat caacctcgat
    21121 gaagcgccct tcatcagcta cgaactggcc atgatcaagg tccgctacga cacgccgcaa
    21181 gtccgcgacg agctggccag cctggccaac ctgttccacg ccaaagtcgt cgatgtcaac
    21241 ttgaactcgc tcatcctccg cgttacagga cgcgaagacc acgtcaatgc cctcatcaag
    21301 gtattgtcgc catatgaaat attagaaatc gcccggaccg ggaccatttc cctctcacga
    21361 ggactcgtac cggtcaaggc gatgtaaaaa aggagctgtc gcatcgcgac agctcctttt
    21421 ttctgtaggg gcgcccacgt ggggcgcccg tccacgcaaa tcgaccacgg gattcttaca
    21481 tatgcccggc gggctcgccc tctacagtgg tccgtttcag caaccgtcct gccattcctt
    21541 gtagtcatgg cctgacgggc tgaaaaggca cgtgcgagcc cctacaaatt ataatgcacc
    21601 ttatcagaaa catccatgtg ccagccacat aaatatgcca taataaggta tcttgtattt
    21661 gatagaaagg gagtgatatg catgtcagct acggtcttga cgacggcttc cccccgtccc
    21721 tgccctttga aatcgccttg ggcgacatcc tggccaaagc cattgccatg tcggcccagg
    21781 ccctccagtc cctgccgaaa gaccagcgcc tgctcatcat cgggacgacg cccatacacg
    21841 gcggcctgct ggtcttcatt aaacatgccg tatcaccgga catcccggac gacaaaaacg
    21901 acctcctccg ggaagcgccc tatgcccagt tcgttccgga cctgaatcag ttctcccggc
    21961 tctacgaggc gaccttccgc tatgacctcc acaaccacct ggccagcctg tatttgacga
    22021 ttccctatca gacttgattg gaagtgatac tcagccatgc tttacaacat cgtgaccgtt
    22081 ttccttatga cgacagccct catgccgcca gctatcctcc agtactatgc ctttgaaccc
    22141 ttgctgaagc atcggcagaa agtcctcgtc ctgggtggat atgccgtcct gtacctcatg
    22201 gaactcatct ttttcctcta tatgttcgtc tggggtccct gggaacacag cttcatctta
    22261 tataaaaaag tctttgtcat cgcctggatt ccccattttc tttgggccgt cgtgacgatc
    22321 cggccttatg tattcctcca tctctacgtc ttttccatcc gcgcagcctg tacgatcttc
    22381 gtccacaccc tgatggccct gatcctgctg gccatcttcc atggcgacag ctcccggccg
    22441 gccctgtcgg cagccatcta tccggcccaa tggctgttct atatcgtctg ctacatggcc
    22501 cttatgccca tgctgcgccg ttatttcaat gaagtcttcg tgaaataccg tcatctgacg
    22561 acgcatcact attggaaata catcagcatg ctgccgctct tcctgctgct ggacatgtac
    22621 ttcatcatgg ccagttcgga cgtcctcatc atggaccgga tgctcctgcc ccgcagtatc
    22681 ctgctcctgg ctttgctcat catcgcccag agcatccgca cgggcctccg ctaaaccgat
    22741 cagaccctga agatgtacga acggacagaa aatatgacag cccaaatcga agccagctac
    22801 gattataccc attccctgct ggagtcacag aaccagatga aggccatata taaaaaatga
    22861 aaggcctacc tggacgaact ggaacaactc atcgtgtccc atcgttccca agaagccttg
    22921 cagctcatcg aggaaatcgg tgccgagctg aatcagacca agcgcaaaca gtattgtcag
    22981 aatcccctgg tcaacgccgc cttgtcgacg tatctggcca aagccgaagc cctgcacatg
    23041 cccattaccg tcaaagccgt catccccaag gaaaatacgg ctctcagctc cgacctggcc
    23101 atcgtcttgt ccaacctcat cgaaaatgcc atccatgcca gcgaaaagca gcccggaagc
    23161 catcgcgccc tgtccctgct gaccatctgc cagggcgaca tcctcagcat cctggtcaag
    23221 aaccgcttcg acgggaccgt agccctcgat gaagacggcc tgccgacgac aaaggtcaag
    23281 ggccatggca tcggcatgaa atcgctgagc cgcttccgcg acacctatga cgccagcgtc
    23341 ctctgcacct gtgaagacgg ctggttctct acgtacatcc gcgtcccctg gggcaagggc
    23401 agcccgtctt agacgacgcc tgtaccgtcg aaggccggga tgaaggcgtc gctggcactg
    23461 cctttttcct ggacgaagcc gtcttcgatg ccgctgtcga agaggtactg ttcggcttca
    23521 tcgtactctt cgtccgacaa gggccggtcg atttcggggt attccccggc ccggccgcag
    23581 ggtacatact ggcgcatcaa actgaataag acctggccgt catcgaagtg gcctcgcacc
    23641 cagtcgatga cggctttcgt attgtctacg cagcccggca ggatgaggtg gcggatgacg
    23701 acgcccttct gcaggatgcc gtcgtcgtct atttcataag ggccgacctg gtcgtacatg
    23761 cgcaggatgg ccgccgtggc gacttgcgga tagtcttcgg cagcactgta gcgcttggcc
    23821 aatttcgggt cgaggtattt caaatcgggc agatagatct gcactttgcc cttgaaatcg
    23881 tcaatcgtct cctgcgattc atagccgccg cagttgtaga cgaccggtac cggcaggggg
    23941 gcggccagac tcgccaagat ggcctgttta tacggcgtcg gcgtgaccag gttgatgttg
    24001 tgcgccccct gggcgatgag ccggccgtaa agctcccgca gcttgtccac cgtaatgggc
    24061 acgcccgtcc cgcccatgga aatggtggca ttctggcaga aacagcagcg caggttgcag
    24121 cccgtgaaaa agacggcgcc agagccgtgg ctgccgctga tacagggttc ttcccactgg
    24181 tgcagggccg cccgggctac gacgggctgg agcggactgt ggcaaaagcc gggcatcttt
    24241 ttttcatccc aaaaggccgc gcggacgacg ccgcagcggc ggggacagtc attacaatag
    24301 gtttccatag ttttctatac ctcagtcaaa aatttttccg ggattcagga tattcttcgg
    24361 gtcccaggcc tgcttcacag ccttcatcaa ggccagttcc accggatccg tatacttggc
    24421 gaacagggcc ttgcgggcct gaccgatacc gtgttccccg gaaatggcac cacccagctc
    24481 ataagctgcc tggcaggcct tatcttcaaa ggcggcgact ttctgctttt catccccttc
    24541 atcggcgaag cccttgcgca ggacatcgag atggatattg ccgtcgccgg cgtggctgac
    24601 accgcggaag acgacgccca gcgcctgtcc caaatcccgt aagcgttcca tgaagagcag
    24661 gagccggtcc ggcgggacga cgaagtcttc catggccgcc acggggcagt ccgcagccga
    24721 cgcttccgta aaggccttgc gggccttcca gactttatcg gcgtcggcca tgaagacatc
    24781 gacagctccc aggcggcggc agacttcatc gacagtgacg cagtcgtcat cgagcgtttc
    24841 gtcccgcgag ctgtcgagct ggataatcat ataggcgccg ccggccgggc agggctgttt
    24901 ttctttgagc cagtcctgga cgacggaaat gacgtcatag tccatgaatt ccaggcacgt
    24961 cggcgtaata cctgccttgg gcagggccgt gaccagggcc agggccgttt ccggcgacgg
    25021 aaagacggcc aggacgtggg ccgtggccct gggcaggggt acgagcttca aagtcagctc
    25081 cgtaatgatg cccagcgtcc cttcggaacc gatgacgatt ttttccaggg gataaccggc
    25141 cgtcatcttg tgcaggcgct tgccgagatg ggcgatctgc ccggtcggcg tgacgacttc
    25201 gatggcgtag atctgatccc gcgtcgtccc gtacttgacg gcccggttgc cgccggcatt
    25261 ggtcgcgcca ttgccgccaa tacagcagca gtcgccgctg caggggtcgc cggcatagag
    25321 gaggccgcgc ttgcgggctt cttcctgtat ccgcgccgta atgacgccgg tctggacgac
    25381 catgtacatg gcgtcttcat tgatttctac gatacggtcc atccgttccg tactgactac
    25441 gatgccgccg cggctgtcgg ccacagctcc gccttccagg ccggtcccgg tccccctggg
    25501 gatgaccggg atgacggcgt cattggccag gcggacgaca gccgccaatt cttcggccga
    25561 cgccggcagg acgacgacgt cgggcagaca ggctggcccg gtcccttcgt cttgtccata
    25621 gggaatcttt ttatcccggt ccgtccagac atatttagcg ccgacgatgg cttcgaggtc
    25681 cgcgacgacg gccggggtta cgtgattata ctccataatg acttccttct caggcttcct
    25741 gcccgaagat aaattttaaa taactttcct ggaactggcg atatttccgc ttggccaggg
    25801 gaatcttatg gccctggatt tcgatgtaat cgctcttgat gacgcggata gccgactggt
    25861 tgacgatata gcctttccca ggcgatacga actggcccgg cgcaatggca tccagcgtat
    25921 ccaagagact agccagggac tgccgcgttt ccagcagcaa gtggtctgcc gtatagacct
    25981 gctggatgtg cccattgctt tcgacgtaga ggatggcgtt gacttcttcg acctggatac
    26041 cgccgccgac gaggcggaag atcatcttgc cgctgtggcg ctggcggatg ctttccatga
    26101 cgcggtccat ggcctggtcg aactctttct gcgtaaaggg cttgaccagg taatgggcag
    26161 cccggacggc aaaggcttcg acggcaaatt cttcgctcgt cgacaagaag acgatttccg
    26221 cccggctctt ctgcttgcgc atctctgcgg caatgtccgt ccccagcagg ccgggcatac
    26281 agatatccag caggacgatg tcgaaattcc cttccttttc aaaagcatcg acgaaatcca
    26341 tggcattgtt ataggtaaag acttcggtcg gttccttttt atcgtggaaa tactgttgga
    26401 ctgccttttg aatgatgtcc aactgttcct gtaaatcatc acaaatcgca attcgtacca
    26461 tcgtgacgcc tcctttcaat aaacaagtat tttacaagtt tattatatca ccagattcgt
    26521 ctcaagccgt ccgatgtgtc acgaaatggc gcatttttga cgcgtagcgg cgttcagaag
    26581 aaggtattta ccatcatgca tggtcccttt ttctctatag taggggcgcc cacgtggggc
    26641 gcccgtcagc atatgcaaga atcccgttat cactgatttg cctgagcggg ctcgccacgt
    26701 gcgagcccct acagaaaagg gactgtcgca catgcaacag ccccttttcc cccaactaca
    26761 gtacgatgcc ccgcaggagc ggtacgatgg tgaactgata taatttcaaa aagccgaaga
    26821 cgcagatgcc gatggatgcg ccgacgatgg ccccgttgat acggatccag gccaggtctt
    26881 cgccgacttt gctttccagg aactggttga accgcttctt gtcgaagccc gacaggacgt
    26941 cgcggacggc gacggcgatg agcgagtgtt catgttccag gatgacgttg caggcccggc
    27001 gtatctgttc atcgagccag tctttgattt ccggatagga ccggcagtat tccaggtaat
    27061 ggatgaactg ttcttccaat aaatccttca gttcttcctt gccctggtcg cctttggtga
    27121 aatagtcctt ggccgaattc tgggtcgccg tcagccactg ctcgatgggc agggagtcga
    27181 cgaactggtc ctgccagttc tggacgccaa aggccagctg gccgttcatg gaatagagga
    27241 agagttccag tttgcggcac agccattcgc gctgttcccc ttcggcatgt tcccactggt
    27301 cgagccattc gacgatgccc atcttgatat ccttggcaat cttacgggta tcgacaatgc
    27361 ccaggacttt ggtcagtgtg aggctggaca tggacgaatg ctgcgattct tcacgccact
    27421 ggaggatgag cttttccagg aggctgtcga agtcgtcgct gttgaccagt tcccgcacga
    27481 ggccgatggc atcgttgaac atgttgctgt tcgggtcttc caaaagggaa atgaggcggt
    27541 cctggaagaa actgaccagg ggctgctgtt tcaggtactt gcggatgtgc gaggctatgg
    27601 atgcctgggt cccacccttc tgagcgtagc ggtaagcccg ctgagccact tcgtagagga
    27661 cgctgcggaa cttttcccgg ccgttcgggc cgcgcagcca gccgacgagc ttatcgatga
    27721 aagaaatctg atagagctta tcctgccaca tattgggctg gagaagcttt tccgagatga
    27781 tcttggtcat gctgtcgatg atctggtcgc gatggcgata gagcagttcc gtgtgatacg
    27841 gaaagcccaa cggcttttca aagagcgccg taacggcgaa ccagtcggcg atactgccga
    27901 tgagggccga ttggatgacg aagaacagca gttcttccca gaccgagacg tcatgcgtat
    27961 accaccagag gaaaatggcg atgaagcaga cgaaagacag gcctaaaatc atattggcac
    28021 gctgtctgta tgtcataaga tagcccctcc cttcacggcg tacatgacca ggtaaatgac
    28081 ggcacccagg atggcgccga cgagcgaacc gttgacgcgg atcatctgca ggtcatacca
    28141 gacactgttt tccagctgtt ctgccaggta attgccgtcg tatttcagga tttcctgttc
    28201 tgccgtcttg ccgaaccata cctggaggcg cttcaa
//
LOCUS       DAWABZ010000011        25574 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_556_length_25574_cov_3.345860, whole genome shotgun sequence.
ACCESSION   DAWABZ010000011 DAWABZ010000000
VERSION     DAWABZ010000011.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 25574)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 25574)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..25574
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_556_length_25574_cov_3.345860"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2178..2187
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10588..10597
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5332 a         6899 c         7583 g         5740 t
ORIGIN      
        1 ccgaggaata cgacggtgtc tacgcggttg cgcgtcgtcc gtgccagttc ttccagggca
       61 aagatgcgtt ccggactgtt gatgttgcct tcttcgatgt acggcagctg cgggaagagg
      121 acttcttccg gttctccgtc tttcgagaga tggcccggca tgatgccggt cttgcgcata
      181 ttcataacgg cttcgtgagc cttggtgtaa acgggttcaa aagccttgac ttcttcttca
      241 gtgacggcct tgggaccata aaggttggtt acgtcaaaag taaaaccaga acgtaatttt
      301 aattgagtca tgtaagttcc ctacctttcg atagaataat gtcatattcg cgtatgaaat
      361 cattatagca caaaacgggg cgcacgggct acctcctgga cggcgaacgg tccgatgtgg
      421 aaaaaggact tgtcgcatga cgacaagtcc ttttgctgta ggggctcgca cgtggcgagc
      481 ccgctcacgt aaatggttga taacgggatt cctttatata ccggcgggcg ccccacgtag
      541 gcgcccctac aatacgatcc acgggattcc cacaagcggt ccatgggcaa cctctcagac
      601 cgcctttggc ggccagctcc cctattagag gagccttgcg gcctgcggcc tgcgaattgc
      661 gaactgcgaa ttatgatttt accatcaaaa atggagcatg gatttgagga agttctgggt
      721 ccgttcgttc tgcgggtgtt cgaagacgtc ttccggtttg ccttgttcct ggatgatgcc
      781 gtcggccatg aagatgaccc ggtcggcgac ttctttggcg aaggccattt cgtgggtgac
      841 gatgatcatg gtcatgtgtt cgtcggcgag ctgtttgatg gtccgcagga cttcgccggt
      901 cagttccggg tccagggcgc tcgtcggttc gtcgaagagc atgatgtccg ggtccatggc
      961 cagggcgcgg gcgatggcca cacgctgctg ctggccgccg gagaggcgcg acggataggc
     1021 atcgcgttta tcccagaggc cgactttttt caggagttct tcggctttgg gcatgatttc
     1081 ttcggtcttc atcttcttga cgatgcgcgg cgcttccagg atgttgtcca ggacggtcat
     1141 gtgcgggaag aggttgaagt gctggaagca catgcccatg cggcggcaga tggtccggac
     1201 gtcggctgcc ggggcgtaga cggcacggct gctcccttcg ggcgtgctga ccatggtctg
     1261 gccgtcgacg acgatggagc cgctgttgat cgtttccagc tgacagagac agcgcaggaa
     1321 ggtactcttg ccgctgcccg acgggccgat gatggaaatg acttcccccg tttcaacgtg
     1381 gagggtgacg tctttcaaga cttcgagact gccgaagctc ttgcggatat ggtccatttc
     1441 aataaaactc atgataatcc tccttattca tcgtaaacgg catagcgttt ttccagataa
     1501 ccaaagacct tggtcaggat aaaggtaaag aacaggtaga agacggcagc gaccaggaag
     1561 gccgtcgtgt cgaagtcgcg ctgtacgatg gagcgcgtaa tgcgcaggat gtcgttcatg
     1621 gccaggatgt agaccaggga cgtatccttg aggaggttga tcgtctcgtt cgagaccggc
     1681 ggcaggacgc gcttgatgac ctggggcagg atgatgcggc gcatggtctg gacgtaagtc
     1741 atgcccagga ctttcgcacc ttcatactgg ccgcggtcga tggactggat gccgccgcgg
     1801 aagatttcgg caaagtaggc ggcatagttg aggacgaagg cgatgatggc cgccgggaag
     1861 gccggcagtt tcaggccgtc gatgatgaag ggcaggccgt agtagatgaa gaggatctgc
     1921 agcatgagcg gcgtgccgcg catgacgtag atataggcgt acatggcctt acgcaggatg
     1981 gcgatcttgg aaatacggcc gatagccatg aggatgccca agggcaggct caggacgatg
     2041 gtgatgatga aaattttcag ggtaatctgc aggcctacgg caacgacagg cacgatgtga
     2101 aataaataat ccatgttggc tccttttttt ggaattgaca catatacagc ataggatacg
     2161 tatatacgca aggcgggnnn nnnnnnnagc agtataaaga cctgcgctcg tgaccggtga
     2221 gcgggccgcc cttttgggcg gcccctacga atcgtcgtta catcccgctt cgcttactgt
     2281 actttaatgg taatatcttc gttaaaccac ttctgggaca acttagtcat tgtaccatct
     2341 tcactcattt gtttcaagac gtcgttgagt ttatcctgca agagcgtatc ttccttgcgc
     2401 atgccgacgc cgaatttttc gtcgcccatc ttgtcgtcga tgactttgaa cttgcccggt
     2461 ttcttggcca tgtagtaacg gccgacgacg ctgtcgacga ggatgccgtc agtacggccg
     2521 atttccaggt ccatgaaggc gttgacgaag tcgccgtatt tcttgacttc tgccagggaa
     2581 tccttgaatt cggcgttctt ttccagggcg tcgatggagc tgctcccttc ctgggtgccg
     2641 atgaccttcc ctgccaggcc ggcccggtca ttgatgggcg aatcggcgcg gacgacgagg
     2701 agctgggcgt tgttcatata gggcttggag aaggcgatct gctgggaccg ttcatcggtg
     2761 atggtcaggc cgttccagag catgtcgacc tgtttgctct tgagggctgc ttccttgctg
     2821 tcccagtcga tgggcttgaa ttcgacgggg atgcccaggc gcttggacgc ttcctgtgcc
     2881 aggtcgatgt cgaagccgac gagctgaccg ctgtcatcgc ggaagcccat gggcgggaag
     2941 ttgtcatcca ggccgatgac gatcttatcg ggcagtttct gttctgcctg tttatttgca
     3001 ttgccgccgc agccggcgac catggtaacg gctgctacgg ccagtgtacc cgcagccagt
     3061 gcttttttcc aattcatgtg aattcctcct tacatcgtga cgtcgatgcc aaaccatttt
     3121 tcagaaatct tcttcgacgt gccgtcgttg tgcatgtctt ccaggacctt gtcgagctgg
     3181 gccttcagtg ccgtatcgtc cttgcgcatg ccgacaccgc tggggatgtc gggatagccg
     3241 acgtcgatga tgcggaacgg cgcgttgttc ttcttgatga agtacgccgc tgtcgtctgg
     3301 gcgacgacga cggcatcgat gcggccgact tccaggtcca tgaaggacgt gacgttgtcc
     3361 gaatactgtt tcagttcctt aaaggaatcg cgcagttccg gttcggcttc gatggcttcc
     3421 aggccggtgc tgccctgctg ggtgccgacg attttgccgg ccaggtcggc cttgccctgg
     3481 atgggcgagt cgttcttgac caacaggatc tgcgggccgt tttcataggg ccgggagaag
     3541 aggatgttct gttcccgttc cggcgtgatg gacaggccgt tccagatggc atcgattttt
     3601 ttgctcttca gttccgtttc cttgctgtcc caatcgatgg gcttgaattc gacttccatg
     3661 cccaggcgct tggaggcttc cttggccatg tcgatgtcaa agccgacgag ctcgccgctt
     3721 tcatcatgga acccaaaagg cgggaagttg tcatcgaggc cgatgacaat cttatccctc
     3781 ttggcagccg tccccttgtc ggcctgtttg tccccgccgc agccggcgac gaaagttgct
     3841 gtcagaattg ccgccatacc ggcagctgcg tattttttcc attccatgat tatcgctctc
     3901 cccttgagat catctaggcg taccgttgtg tacatgccct attttacttt atttcgttaa
     3961 aaagataaag gcttttgggt ataaaaaatc ggcatgtctc catgccgatt catgcatatc
     4021 gcacatagga caggtcctgt caatgggcgc tttcgtcatc gtccgtttca tgggcctgct
     4081 tgatgcggtc gatgatgcgc tgtttgttgc gcttggacag gaactgcagg tgcacgggca
     4141 ggacggccag gccgccttct tcggcttcgc ccagctggat ttcctgccag ttgtcgctga
     4201 tgacgaagat gtccttatac gggaggaaga tgaggctccg ctcctcatgg aactggtcgt
     4261 cgtagaattc atggagcgac cggaactggc ggcggacgat gaggcccttc cgggtgacgt
     4321 agcacatgac gtcgtcctgg cgatggatgc gccacaggca aaaggccagg acggcccagt
     4381 tgtcgaggtg ttcaaccagg tgcgcttccc ccgtcatgag ccagaagaag tagatggcca
     4441 ccatgaggaa gaggaccttc atgaagccgg aaaaccattt cgagttctca taggccgcct
     4501 cgacgggacc acagcggaaa tgcttttcca ggtctgcctg tttcattttc tcagtttttt
     4561 gctcgtcttc ggtgccgtct tgatgacgac gttgcggccg tccttgacga cttccaggga
     4621 gatgtcgccg acgccgtcct tgaagtaagc catcttgata tcgaggagga tagagccgac
     4681 tttttcctgg aggtcgtccg tgaagaactg gcggcggaag tccgaggcgg tctgcttctt
     4741 agccgtcttt ttggcctgcg gccggcggac gctcacttcg tcgtcttccc agcccgtcgt
     4801 ttcaaaggtc gattcttctt cgacgccctg gaacatgtct tcgatagagc gattcttggg
     4861 aatcttatgt tttgccatga caatcaccta cttcttttcc attttatcgg atttttccga
     4921 tgcttcgtgc atactgttaa gggtaatctt attgaagtct tccggcgtct tgtacgtcgc
     4981 tatcttcttg aagccgaccc gcaggcggcc gcggtaggcg gctacgacct tgtcgtcgtg
     5041 gttcatttcg gcgatgtaga tttcctgtcc atcgataccg atgagacgga aattgccgct
     5101 cggcgagatt tcacgccagc tgctggtcgc cccgtcgaac atgtagacga cgccggtccg
     5161 caggttgtcg tagaagaacg tatccgaatc gtagaatacg gcgatgcggc cgatgttttc
     5221 tgcctggacg tggatgcggc gcgtgtcgta gttggcgtcg gtccggtaga tgcggtattt
     5281 attgtcgccg acctggagct tcatgtagac gacattggtc gccgtcgaat aggcgacatc
     5341 ggtgatctta ctgtcgaccg gggcatcttc gataggcgta tcgagttcgc ggtccggata
     5401 aatcgggttg tactgggcga tgtagacgcc gtacttgccg gtcttcctgt cgcggccata
     5461 ggtggcgaag atagccaggt tgcggtcggg cagccattca aagaaggaga ccttgcggcc
     5521 gtggagattg atgcgttggg gatcgttctt cttgccggct tcgtaaatgg tgaccctgtc
     5581 gtcgacgatg gtcgccatga agcgcttgtc ataggaatag aagctgcggc cgttcggttc
     5641 tgtcccttcg cccatggctt cctgctggtt cgtgccgccg atctggaaat ccgacgtcgg
     5701 cgcgaacaag acctggtcca ggtagagata aatagcagcc tggacggcga tgccggccag
     5761 aaagactgca atgaactttc gtaatttcat agcctgcctc ctacttgacg acgaacgccg
     5821 tcggaatcat gcgttcacct aagaggtccg ccggtttgtt gatgactttg ccattataat
     5881 acagcgtcga gctcgacccg ccatccaggt tggccgcaat gacggcgcct tcttcataca
     5941 tgatgttctg gatgtcgcgc agtgtcgccc cgacggaata gccaggctgg cggccgtcga
     6001 tgaccaggaa gaggaccgtg ccgtctttgc gctggccgat ggccgtgcgg ggaccgacgc
     6061 cccagccgcc gtcgccgtgg gtgatcatct tttcaccgtt gacgatgagg gccggcccga
     6121 aggaaatgcc ttcgacgacg tccttttctt tcagttcggc cttggagtag ttgccgacga
     6181 tgaggttgcc gtccttggta aagccgacga attcattttc ttcctcatcg gacgcatcct
     6241 ggccgaggag atatttgcca tcgtggagga tgaaaccgta aggctggcgg cccgtaccgg
     6301 tcccgttggc atcatagaag ccgccggcat tgatggccgc tacggcgccg acgcgttcgg
     6361 caataccgct ggtcgtgtcg cctttttcat ccatattttc agccgtcgcg acctgtacgc
     6421 ggtgcgggtc cgggatttcc aggaggaatc ccttgaaacg gctggaatca atattcttca
     6481 gggtaagtgt cgagtccgtc cgggctttga acttgaacag ttcctgcttc ggcgtcgagc
     6541 tgatgccgcc caggatttcg tcgatttctt cctgcgaaaa aagccaggtg atgtactggg
     6601 gatgacggct cttcaggata gccccgatga cggtgcgctt caggttgtcg aaggggccga
     6661 agaggacgac aaacggcgat gtcaaggcaa agaaaatggc gacaaagaca aaaaacttac
     6721 cgatttcttt tacgtgtttc ctcataagta acctcgaaaa taataaagtt agcgttcttc
     6781 atactgcggt gtcgtgacgc gctgcttgaa gttttctata tcgacggcat tgccgatcca
     6841 gatgcgcttg accgcatagg ggttgtcccc gtatttggcc cagcccggat ccatggtaaa
     6901 ggccagtttg ataccggctg ccttcaacgc gtcgccggcg cttttcgtat agccgccata
     6961 gggatagcag aaatattcgt tggtaatgtc cagctgttcc ttgagttcag cctggctctt
     7021 cttgatttca tccgcaaagg tgcgccctgt catttccgtc gcccgttcat ggtgataact
     7081 gtgggaggca atggtcaggc cggcagcctg catttccttg acctgctgcc agttcaggcg
     7141 cgttccctgg tccgtgtcat acgtcggcac gaagaccgtc ccggcaaagc cgtattcctt
     7201 caaaacaggc atgacgatgc tgtacgtatc gggatagccg tcgtcaaaag tgaggacgac
     7261 cggccggact ggtacgggct tgttatgcac catatagtca tagagctggt ccagggtaat
     7321 gggatggaag tcattatcct tcaggaactt catctgttcc cggaagtgcg attccaagag
     7381 gaccgcatcg ttatcgggga catcgcctat catgtgatac atcaggaccg ggataccctg
     7441 cttgggcacg gccatttcca cctgcgcctg cggtgccttg tcggccttgg ccgtatcgcc
     7501 gctctgcgtc ggcgtgcagc cgaccagcag gaagcagcac aggatggcca ggcaggccag
     7561 tttccatata cgcaaattgt acagcccctc tcgttaagat tcttcgttgt cccgcaaggt
     7621 cacaacgggc agctcatcgt aatggccagc caggaacagg cggatgtacc gttcggcatc
     7681 atcgcggacg gccttgtcgg cttcgacgga atggaagcag acgctgggcg tgagcaggaa
     7741 ttccggccgg gcgatcatct tgagcagcat cgggctcgga tgttccatgg acaatacgtc
     7801 gagggcggca ctgcggatat ggccgtcgtc gacagcatga taaagagccg cttcgtccgc
     7861 caggccgccg cggcagcagt tgacgaacat ggagccttcc ttcatttcct cgaactgttc
     7921 gtcctggaat atgtagcgtg tcgtttcgtt gagcggcatg tgaagtgata cgacgtcgct
     7981 ggtctggaga agtttgtcga agtcgacggg acggacgctg cgttccatca tgaccttttc
     8041 gctgacaaac ggatcataag cctggatctt gcatccaaaa gcctggagac gggcggcgac
     8101 ggcacgaccg acatggccaa acccgacgag gccgatgaga ttgctgctga cacggtggat
     8161 aggccaggga acggacccgt attcccaacg gctgttggtg cggatatcgc tctggtactc
     8221 cggcagctgg cggatgaggg ccaggatcat ggcacacgta tggtcggcaa tatcgtcgac
     8281 acagtacgac gtattgttga tgaccatgac acccttttcc cgggcagccg ccaagtcgac
     8341 attatccgac cgcgtcgata agacgacgat caatttcaga tcgtccaatt cttccagaag
     8401 ggatgccgta atcggttcgg acttacagat gatgacatct gcatcttcga gcctgtcggc
     8461 caattcttct tcatctccgg gaggacaata ttcaacgcgc gctacatcgc gcagtctctc
     8521 tccaaaatca tcttcatcct gacgtcctgt caaaacactt atgtgataca ttatcactgc
     8581 cccttttcta taaacaaaag atttagacat aaagatacac ttaattatat accatataaa
     8641 agtcaattag caagtccctg tgccgtcctt gacggagttg tgaaaactct gctataatag
     8701 tttcgttatg gaaaggtgtc cgagtggttt aaggagctag tcttgaaaac tagtgatgcg
     8761 gcaacgcacc gtgggttcga atcccaccct ttccgccaat ttccaaaaag ccctgtcgcg
     8821 taggtgacag ggctttttga gtggttagca gcttagtggc taggaattag caaaggcccc
     8881 caatcggggc ctttttatga tttacaatct gtaggggctt gcacgtggca agcccgattc
     8941 aggatatgtg cgattatcca attagttttt tagagaaccg ttcaaatgtc cggcagggcg
     9001 cactctgggc gccctctact atttcttatg gaaaaagtgt ggcgaatcgc cccattgtcc
     9061 caggccgatg gtgtcgccta cggaggctac gcgggcgctg aggcagttct tgcactggtc
     9121 cggtacttcg tcgacacagg gtttgttgtc gtataagagg tactgtgggc gatatttggg
     9181 gaccgtgacg atgggcatga ggacgttggc cccggctttg aggcccagtt cccgtcccag
     9241 gggatggagg gcctggaggg ccgtcgtagc ggcgatgttg acgtcgggca ggaagaggcg
     9301 cgtgacggca atcatcttga ggcccagttc caggcgccgt ttcttttctt cggcactgtc
     9361 cataccggca gccatgacgt ctttgccgac cggtgtattg tgatggacga cgtagggacc
     9421 catgccgatc atgtcgatgt ccatgtcccg gtaaaagagg atgtcatcgg ccaaatcgcc
     9481 cagggtctgg ccgggcaggc cgatcatgtc ccctgtcccg acctggtagc cgatgcggcg
     9541 caggctgtcc aggcagtcct tgcggacctg ccagcggtga tggccgtcct ggggatggag
     9601 cgtcgcgtag aggtcggggt tgctcgtttc gatgcgcagg agatagcggt gggcgccggc
     9661 gtcgaaccag cagcggtacg tttcttccgt ctgttcaccg acgcagaggg tcaggcccag
     9721 ttcgccgtgg ctcaaggcct tgatgtcgcg gactaggcct tcgacgtagt cgatgaactc
     9781 ttcatcctgc cgttcgccgg actggagggt cagggagccg tagcggttct cccaggccca
     9841 gcgggccatg gccaggatgt cttcgcgcgg cgtatcgaag cgttctactt cgtcattgct
     9901 cttgcggatg ccgcagtatt tgcagtcttt gatacagtaa ttggaaaatt cgacgaggcc
     9961 ccggtaatag acgacgcgtc ccacaccggc ggccttgacg gcataagccg catcataaag
    10021 cgcctggagt tcatcaggat cggtaatcga gagcagcgcg atcaaatcgt cgcgggtttt
    10081 cggttgtgga atagtcatag acggaactcc tttcagatgg cctgctattc gttggcccaa
    10141 tgaatctttt caaagaaaag gaagataagg ctcatgacca tggcgacgac cgtagccaga
    10201 cccatgccct tgagctgaac cggaccgagg acgatagtcg ccccgctgag gccggaaatc
    10261 atggtaactg ccgtgaggat gaggttcttc gagcgcgtat aatcgacttt cttttcgacg
    10321 agcatgcgca ggcccgaagc ggcgatgaag ccgaacagga gaatgcagac accgcccatg
    10381 accggcgtcg ggatggcgtg gatgagggct gcgatcttgc cgacgaagga aaagaggatg
    10441 gcaaagacag cagcgccgcc gatgacccac gtactgtata cgccggtaat ggccatgacg
    10501 cccatgtttt caccatacgt cgtattcggc gtcgaaccga ccaggccgga caggacgttg
    10561 gacaagccgt cggcaaagag cgaccggnnn nnnnnnngtg caggcccggg tccttgatga
    10621 ggtcgcggcc gacgatatcg ctggtgacga agagatggcc caggtgttcg gccaggacga
    10681 cgaacagggc cggcatgatc atcatgatag cgctgagatt gaattccggc ttatagaagg
    10741 tcggaaaagc gaaccagggc atttcctgga catgggtcca atcgacgacg cccatgaaga
    10801 acgacaggat atagccggca atgacgccga tgaggatggg gatgatgccg atgaagcccc
    10861 ggccgaggac ggtggccagg atggtgacga ccagggtgaa cgtcgaaatg atcatggccg
    10921 tttcattgga catgccctgg acttcgccca tataaccgga catattcatg gccagcggcg
    10981 ccagttccag gccgatgatg gctacgatgg agcccatggc agccggcggg aagaggacgt
    11041 cgatccactt ggtgccgacc ttgtcgacaa tggccgccag gatcatgaag gacaggccga
    11101 agacgatgaa gcccccctgg gcgtcgccat aggacatgcc cggtgtcgcg cagacagcca
    11161 ggaccggcga aatgaaggcg aaactcgaac cgaggtaggc cggcaggcgc catttacaaa
    11221 tggtcagata aatgagcgta ccgacgccgt tcatgaacag ggccgtcgcc gggtcgacgt
    11281 ggagcaggaa cgggaccagg accgtcgagc cgaacatggc aaagagatgc tgtaaactca
    11341 ggggaatcgt cggcaagata ggcagccgtt cttccacatg tataattctt cgttgttcca
    11401 taaaatcaaa atcctcctaa attcaatgcc ccataaaggg cggacgcaaa gcctgccggg
    11461 cctgctgggc actcaagtcc tgcgccttgg ccgcggccat gtaattatca tagctgacgc
    11521 gcagcaggag cgacgcagaa atcaggttca aaataaggga tgacccgccg taactgatga
    11581 agggcagcgg gacaccgacg acgggaaaca ggtccgtaac catagccatg ttgaccaggc
    11641 cctggccgcc gagagaaaag gtaattccca tggccaggaa catgccgaac cggtcctgac
    11701 aggagcctgc cgcctggacg ccaaaataaa tcagggcgaa aaacaggaat aagaccagga
    11761 caccgcccaa aaagccccat tcctggcaaa ggatagcata agcgaagtcc gtatgggctt
    11821 cgggcaggta actgaacttg ctgatgcccg tccccatgcc ctggccccag acgccgccgg
    11881 aaccgatggc gatgaggctc tgggttatct gatagccgat ggtcttcttg tagtcctcag
    11941 ggtatatcca ggcccggatg cggtctttct ggtaatcatg accaaagaaa taaaaagcca
    12001 ggacgccgat aacgattgcc gcaagggtcc acaggccgat tttcttatac gaccagtgcc
    12061 ggtcatagag tgggatgccg ctgcaaaaga gcatgaacgc cgacggcaca aagagcacga
    12121 tagccgtgcc ggcatcaggc tgaatcaaga taatagcaga gaggaagaga gccggcaaga
    12181 ggcagagcag accatagctc ttgaagagct tcttctttgc caataacgac ggcgccaagt
    12241 gctcgatgcg ccatttttta gtgaatcgtt cgacgacagt ctgctggtca ccgtgacatt
    12301 cccaaaagaa agtcagcgcc gcagccgtgc aaatgatggc tgccagctta gcaaattccg
    12361 acggctggaa agacacggcc ccatagccca gccagcgctg agcaccgttg acggtgatgc
    12421 cgatggcctt gacggcaaag agcagcacgg cgctgatcag gaccagcgta ccgcaccagc
    12481 ggcgcagcca cggatagccc aggtgccaga cgccggcaga cgccagcagg cccatcacca
    12541 ggaagcccaa ttgtttcaag agataaccat agaccgtact gccgccatcg aggacaaagg
    12601 tcgagctgag gatattttcc atgccgatga cgacgaggca cgaaaagata cccaccagcc
    12661 agaggacgtt agtcctccgg cgccgggtcc ggacggtcag tctgggtttt aacatgagca
    12721 atcacttcct tgggacgcgt agcgacacaa tggaaaatgg gctgtccctt ggcgctgaac
    12781 ttcgcttcat attccgtcat ggcttcattc aaaatcgggc tgttatgcag atcataagtc
    12841 acttccgaca agggccagcc acattcgcgg aactcgtcga gggtgaaatc gaagagaccg
    12901 gcattatcgg acttgaagta aatctggccg ttttcccgca ggagccgtgc gtagcggtcg
    12961 aggaaaccgc ggtacgtcag gcgccgtttg gcatggcgct tcttgggcca gggatcacag
    13021 aagttgatga agaagcggtc cacttcccct ggggcaaaaa tatcttccag gcgggctacg
    13081 tcaaagcgca ggagctggac attgtccact tcggcagcgg cacacttctt gccggcataa
    13141 tagaggacgc cgacctggat ctcgatgccg atgaaattga catccttgtg gatctgggcc
    13201 atctgggaaa tgaaattccc cttccccgtc cccaattcga cccacagcgg cgcatcggga
    13261 ttcttaaaga gggaacgcca ttttccctta tagctttcgc agctttccag atggagaaaa
    13321 tcactataat ccttgatagc ctcgtcaatc cacggttttc ttcgtaaacg cattgtacct
    13381 cctatgtaaa aaacgattca tcgtacaaga acccttcaga cctgctgtac aagccgaagg
    13441 gttccatgac taactttttc agacttattt aattataaaa aatgtcggcg ccggtgtcaa
    13501 tcttttaatg gcagccagcc atcaatggcc gtaatgccca ggcccggtgc gtcggggacg
    13561 acgaggtggt ctttatcata ggtgatgccg ccccggacgg ggtcggcggc catgaggtcg
    13621 gcggcgtcga ggtcgttctt ggtgatgacg gccttgccgg cagacagggc cgcagcggcg
    13681 gtgatgccga ctttactttc cagcatacag cccatcatgc aggcgacgcc ggcggcgtcg
    13741 gccatggcgg cgatctgggc agccggggcc aggccgccgg ccttcatgag tttgatgttg
    13801 atgaggtcac aggcccgcat ggccaggagg cggaagacgt cgcgcgggct gaaagccgat
    13861 tcgtcggcca tgatgtccgt cgcgacgtgg tccgtgacct gtttcaggcc gtcgaagtca
    13921 gcggctggca cgggctgttc gaccagttcg acagcgaggc cggcgtcttc catgcggccg
    13981 atgaggcgga tggcttcttt cggcttccag ccctggttgg cgtcgaggcg gatagtgacg
    14041 tccggcccga cggccttgcg gatggcggcg acgcgctgga agtccatggc cgggtcgatg
    14101 ccgactttca atttcaagtg gctgtagccg cgagaaacgg cccggacggc gtcggaagcc
    14161 atcgtgtccg gcgcgttgac gctgatggtg aggtccgagg cgatgcgcgt cttgcggccg
    14221 ccgaagaagc ggtacagggg caggccgtaa cgctggccga agaggtcgtg gacggccatg
    14281 tccagggcgg ccttggccga cgtgttgtgg accatagatt gatggatcgt gtcgaggata
    14341 tcttcgcggt cgtcgatatc ccggccgatg agtttcggcc cgatgatata tttaatggca
    14401 gcggcgaccg attggtgact gtcaccggtg atgacgaccg tcgccggtgc gttgccgtag
    14461 cccgtgtggc cgtcgtcggt atggaccttg acgaccaggt cttcggcgac gtcgacgcgg
    14521 cgcagggccg tgatgaacgg cgtcttcaag ggaatggcga tcttcccgat ttcaatagct
    14581 gtaatcttca tgataattcc tacggtaaaa tatctaaaat ctcttcgact gaatcgacga
    14641 tgtagcggac gtgctccttc tggaacatgg gcttggccgc cggcccgtcg atgccggtca
    14701 gggtcccgat catggtggct cccatggccc gggcgcacca gacgtcggcc agggagtcgc
    14761 cgacgacgta gtagatgccc ttcttgaagt cgtcgggatg gctgacgtaa tcgaggtaat
    14821 gtttcttatt cttgccgaaa gccccgaggt aataagcaaa ggggttgggc ttgtccaggg
    14881 acatgtgcag catggcttcg gcttcttcga tgtcgctata ggtcgcaacg tagtgttcgt
    14941 cgaattcgtc gagccagcca taggtccgga agggaatctc catttccatt ttcgaccggc
    15001 ccgtggcgat ggcaatctga tagccccgtt tcttcaattc ccggaacatg ttgcggatgc
    15061 cgtcaacagt ccccagtggt tcttcccgcg tcaaaaagcc cggtttcccc ggtgcataag
    15121 gtttcttgcc atacgtctgt tcatagagct cgtcgccgaa ataccagtcc tggaaggcct
    15181 cgaattccag ctgccacaag ggcgaatcga ggggcgtcca ctgcttggag cccggtccca
    15241 gggacgactg gacggcattg gccagatagg taaagacttc gtccttgccg gcccgttcgg
    15301 ggatgacatt gctcaggcgg cgatagacgt cgtcggccgt cggtacggga tagccccgca
    15361 ggagagagcc caggtactgg atatcgtcga ttttcttcag gctcccttcc agaccgccgt
    15421 ggtcggcgac atacttttcc agcatgagcc acaaggtgac gacgatgtgg gcgtggacca
    15481 tgtcccagtt gctgttgacg ccgtgtttct tgagccacaa taagatatcg tcgttcctcc
    15541 agaagcgggc ccgcagggcg gcgatggtat catcgtcaag gtcggccgtg atccgttccg
    15601 gtttgaggtg catgtagcgc gggctgtaat accattccca gactaccagg gccgatacgt
    15661 cgaaatagcg tttttcactg agcaagacgc catcgacgtc aaaaatcact ttcttcatcg
    15721 cgccttcctc ctcttgctta aaatgtcttg tccaaatggc cgacagtctt cttattgccc
    15781 ttgacatggg accgttcttc caggaccttc atgacatcgt tgacggtgac gcccgtgtcc
    15841 tcccagagga cggacaggtg atacaagagg tcggccgatt ccttgcagac ttccaggcgc
    15901 aggttgtcgg cggcttccga cgtatccttg acgacggggt cggcgttctt ggcggcgatg
    15961 atgacttccg acgattcttc gccgattttc ttgcagatct tgtcgatgcc cgtctgcagg
    16021 aggtaagccg tgtaggattt ttctgtcgga tggatgcgtt tttccttgat ttcttcgcgc
    16081 aggtccgaca gcatggacaa gttgcccgta taggtctttt cccattccgt catggaccgg
    16141 aagaaacacg atttgtggcc tgtatggcag gcggcgccga cctggtcgac ggtcaggagg
    16201 atcgtgtcgg catcgcagtc gacggcgatg gagcggatgt gctggaaatg tcccgacgtt
    16261 tcgcctttat gccacaattc ctggcggctg cggctgtaat accaggcatc gccactttcg
    16321 acggtgcgct tcagggcttc ggcgttcata taggccacca tgaggacctt gccgctgcat
    16381 ccttcctgga cgacggccgg cccgaggccc ttttcatcaa atttaatgtc tttcatcgat
    16441 acactttcca tttatttttc tgcccctctc atgggaattc cctgtccctg taaataggtc
    16501 ttcaaatcgg ggatgtcgat ctggccgaag tggaagaccg aagcggccag ggcggcatcg
    16561 acgccgacgt cgaagacatc cttgaaatcc tgcatcgtgc cggcaccgcc ggaagcgatg
    16621 atgggcacag gcagttccgt cgccaggcgg cggttcagtt ccaggtcgaa gccggccttt
    16681 tcgccgtccg cgtccatgga attcatgcag atttcgccgg caccgagggc gacgccctgt
    16741 ttggcccagg cgatggcgtc gatgcccgta ttcttgcggc ccccttcgac gtagacctgc
    16801 cagctgccgt ggttatcgcg cttggcatcg atggacagga cgacgcactg gctgccgaag
    16861 cgctgggccg cttcgcgtat cagttcggga cggcggacgg cagccgaatt gaccgatacc
    16921 ttatcggcac cggcccgcag gacctggtgg aagtcgtcga tgctgctgat gccgccgccg
    16981 atggtaaagg gaatggtaat ggcttcggct acggcgcgga cgacgtcgat gaagatatgg
    17041 cgcttttcat gggtcgccgt gatgtcgtag aagacgagtt catcggcgag ctggtcggaa
    17101 tagaatttcc ccaatttgac gggatcgtcg acgtcctgga tatgtttaaa ctgcttgcct
    17161 ttgacgacgc ggccatcgcg gacgtccagg cacggtacaa tacgttttgc caacatctta
    17221 cattccccct aatgcgcgga tttcggccat cgtgattttc ccctcgtaga gcgccttgcc
    17281 ggtgatggca ccgtagacgc ccatggcctg taatttcttc agatcagcga tgctgctcat
    17341 gccaccggac gcgatgaggc ggatgaaggg cagctgctgg agctggctga gcatttcaaa
    17401 gttcggccct gtcagcatgc cgtcgcggct gatgtccgta tagaccaggg tattgatgcc
    17461 gatgtcgagc atggactggg caaagggcag gaccttcttg tcactgccgt cgacccagcc
    17521 gcgggtagcg acgacatcgc ccttggcgtc gatggatacg gccagggacg agccgtattt
    17581 cttggccata gccgtaatga attccgggtc ttcgacggct ttggacccga tgatgacgcg
    17641 gttaacaccg gcttccagat acgtttcgat agcttcttcg ctgcggatgc cgccgccgac
    17701 ttcgacgggg atatcgacgg cttcacagat gtcgccgatg atcgaggcat tggtcagttt
    17761 gcccgaaaag gcgccgtcca ggtcgacgac gtggagaaac atggcccctt cgtcgcacca
    17821 tttgacggca gcgtcgacgg ggttctcaaa ataggtcgta ctgtcgtcct tgacgccctg
    17881 gcgcaggcgg acagcatggc cgttctggat atcgatagcg ggaaatacga tcatagtctc
    17941 accctttctt catgacgtaa tcgacgacgt tccggaggag ctgttcgccg acgcggccgc
    18001 tcttttccgg atggaactgg aagcccagga cattgtcttt gccgcagata gccgggacgg
    18061 gctggccgta atcggccgta gcgatgacgt cgggcgtatc cactggcgat ttatggtagg
    18121 aatggacgaa atagacgtaa ggatgagccg gcagtccttt ctggagccag tgttcctggc
    18181 ggaattccaa ctcattccag cccatgtggg gcactttcag tttcgtgtgg aagggcacga
    18241 tgtcgccggg caggaaaccc aggcagtcgt aggtgccgcc ttcgtagctc ttttcaaaga
    18301 gggcctgcat gcccaggcag acgcccatga agaacttgcc gctctggacc tgttcctgca
    18361 ggagcgggat gaggtcgaat ttctgtaagt tccccatggc atcgcggatg gcaccgacgc
    18421 cgggcagttc gaccaaatca gcgtcgcgga tgacagccgg gtcgcgggta atggccgctt
    18481 cgatgcccag gcgtttccag gcattgtata cgttggccag gttgccaact tcataatcga
    18541 taatcgcaat cttcatcata acaccccttt tgtcgacaag acactcgttt cgtcgccttc
    18601 gatgcggacg gcttccttca aggcatggcc cagccctttg aacaccgatt cgacgatgtg
    18661 gtgggcattt tcgccataga gattggtgac gtgcaggttc atgcggccgt ggacggccag
    18721 ggcatagagg aattctttga gcatttccgt ttcaaaggtc ccgatgcgtt cgacggcgag
    18781 tttcacgtcg tagaccaggt agggccggcc gctgaggtcg atgacgaccc gcgtcagggc
    18841 ttcgtccatg gggcagtaga acatgccgta gcggttgatg cccttcttgt cgcccagggc
    18901 ccgggtcagg gcgtcgccaa agacgatggc caggtcttcg atggtgtgat gcgtgtcgac
    18961 gtcgagatca ccgtggacgt ggagagtaat gtccatgccg ctgtggcagg ccaggaggtt
    19021 catcatgtgg tcgaagaaac cgatgcccgt cgtgccaccg aaggtaccgg agccgtcgat
    19081 gttgagggag acggcgatct gcgtctctgc cgtttcccgt tcgacctggg cactacgcat
    19141 atttcttcgc ctcctcggtc atgacggcga cgacgcggcg gacgacgtcg tccgtggtga
    19201 tggtgatgcg caggcagttc ttgaggtctg ccgccttata ggagcggacg cagatgtcgg
    19261 cgtcgtgcag ggccttgacc atggccgggc cgtcggggac gcggaccagg acgaagttgg
    19321 tcgcactggg atagaccgta agattcggca cgtcctttaa ggcgtccatg aaatgctggc
    19381 gcgctgccag ggtcggcggg atattgtggg ccaggatgtc atcggcatag tccatggcgg
    19441 ccagggcagc gttctgcgag aggatgttca ggttgtagac gagtttcgtc aggcccaaag
    19501 cgtcgatgac ttccttctgg gccacgccgt agccgacgcg gcagccggcc aggccgaagg
    19561 ccttggacaa ggtgcggatg acgatgaggt tgtcgtactg ggcaagtttg gtaatgatgc
    19621 tcggcgcgcc ggcaaattcc aagtacgctt catcgacgac gacgatattg tcggcagcct
    19681 ggaccaggct ttcgacgtaa tccaggggca gcagttcccc tgtcgggttg ttcgggttgc
    19741 agaggaccgt cactttcggc tggagttcct tcactttggc caggaggccg tcgcgattgc
    19801 ggcggtagcc gtcgaggtcg gacacggtga cgaccttggc accgaggact tcggcgtcga
    19861 tgccgtagat gtcgaaggtc ggcgtatggg tcaggagcgt atcgccggga ttgaggaagg
    19921 tattcatgat gaggctgatg atttcatcgc cgccattgcc gacgaggact tcctgaggct
    19981 ggcatccgac gtatttcgcc agggctgccc gcagttcttc ggcaaagggg tccggatagt
    20041 ggtacgacgg cgtcttggcc aggcgttcca ggaaatcggc tttgaccttg gggaaatcaa
    20101 agaggttgta cggactttca ttggcattga tgaccgtatg ctcccggatt tccgggacga
    20161 catagggttc aaaagagcgg atggtattgc gaagccagtt attattcatc gattctcctc
    20221 cttacagcca ggccgtgagc ctgcaatcct tctacattgg cgaagcgcat gaccttgtcg
    20281 gcgacagctg tgaaagcgtc cttatcgtat tccaggaggc tgctgcgctt gacgaaatca
    20341 tagacgccga ggggcgacga gaaagccgcc gtccccatag tcggcagggt atggttgggg
    20401 ccggcgaagt agtcgcccag gggttccgtc gtatagggcc ccatgaagat agcaccggca
    20461 ttgacgattt tcttggccca cgtttcggcg tcgctgaagg cgatttccag gtgttccggc
    20521 gcgatgaggt tcataatatc tacggcctgg tccttatcct tgacgatgaa gaacttggcg
    20581 tgtttttcaa tggattcggc agcgatgtcc ttgcgcggca gttcttccag ctgacgccgg
    20641 acttcagctt cgacttcgcg ggcaaaggcg gcgctgggcg tgaccaggaa gacggcggcc
    20701 cggcggtcgt gttcggcctg ggccaggagg tcagcagcga cccagaccgg attggcgtcg
    20761 tggtcggcca ggacgccgac ttcactgggg ccggcgatca tgtcgatgcc gacagtgccg
    20821 aagaccaggc gcttggccat ggcgacgtaa gcgttgcccg ggccgacgat cttgaagacc
    20881 ggcgggatgc tttccgtacc gtaagcggca gcggcaatgc cctgggcacc gccgacttta
    20941 tagatttccg tagcgccggc gacatgggcc gcagccagga tgtagggatt gaccgacccg
    21001 tctttggcag gcggcgtaaa gacggcgatg gacgggacgc cggcgacgtg ggccgggatg
    21061 gtgtccataa ggaccgtcga cggataggcc gccgtgccgc cggggacgta gacaccgacg
    21121 cgctggatag gcgtgaactt ggcccccagg cggacgccgg gacggaaggt gtcgatccag
    21181 gtctgttcga cctggcgttc atgataggcc cggatgttgg cggccgcttc ttccagaatg
    21241 gccatgaaat cgggaccgac gatgtcgacg gcatcgtcga tttcttccgg cgaaacgagg
    21301 aaactgtcga ggtcgacgcc atcgaagcgt ttcgtatatt cccggacagc ggcgtcaccg
    21361 cgctgttcga tgtcggccag gatagcttcg acagccttgc ggatgtcttc actttctgcc
    21421 tggtcccgtt tagtaatgcg gcgaatgtcg cgtaaggtca acccttcact gggtgcatct
    21481 atccattcca ctctaactcc cccttagtta tgccttcatg ctgtgttcac ggatgacatc
    21541 catgagctgc tggatttcgt tatatttcat cttaaaggaa acgggattgc aaatcatgcg
    21601 ggcactgaaa tccatgaagt atttcaggac ttccaggccg ttggccttca aagtattgcc
    21661 cgtttcgaca atatcgacga tgacatcgga caagccgacg atcggtgcca gttcaacgga
    21721 cccgttgagc ttgatgatgt cgacgctgtg atgattggcc tcgaaatagc tgcgggcaat
    21781 gcgcggatat ttcgtgccga tagtgatgcg cttggtcatg tcgaccttct tgccggcgat
    21841 accggcgacg gccatgcggc agcggccgat gcccaggtcc atgacttcgt agacgtcggc
    21901 gcccggcgct tcttcgatga ggacgtcctt gccgacgata ccgacgtcag cggcgccgcg
    21961 ttcgacgtag acagcgacgt caggtgactt gaccagggtc actttgagct ggcccttggc
    22021 gtcttcaaag atgagcttgc ggctctcttc ggaatacgtc gggaaatcat agccgctttc
    22081 ggctaaatac ttcatgacca ctttatgaat acggcccttg gccagtgcaa tatgaatcgc
    22141 catgttacaa cctccccatg agtgcttcca attcttcggc cgtcatggcc ttgccgtcct
    22201 tatagtacga gccgttgcgg taaatagcgt ggatactgta atcgccgctg tcgatgaagg
    22261 acgaatcggg ataggcggcg acgcggaagc cgcggctgcg ccagttcatg aggtcccgga
    22321 ccagggtctg gtcgccggct gtataggata cggccatctg gaacgacggt tccgtatctt
    22381 ccaacaggcc ggcatcgttc atgtattcat acaataaatt gatgttcatg ccgaagccgc
    22441 aggccgggcg gtcgacgccg aaccgcgacg acagctggtc atagcggccg ccgtcgatga
    22501 tgtcgtacag agcgccgtcg acgtagacct tgaagaccgt atccgtatag tagcccaggc
    22561 ggctggcaaa gccgaagtcg agctggacct tatcggcata gccggcataa aggatgtagt
    22621 catagacccg ttccagccgc gacagggcgt tgagcatgcc gctgttgata gagtaatctc
    22681 gggccttgct cagggtctgg gcatagggcc cgaagaggac cggcagggct tccagggcct
    22741 tgcgggcatc ggccgatacc ggcagggcgg cgatatagga cttgcaggcc acgaggttgc
    22801 ggtcggccat atagcctttg agcgtttctt tctcttcttc cgaaatggga aggccatcga
    22861 acaatgcctg cgtataggct gccgtaccga tgtcgatgcg gatgttttcg acgccgacgg
    22921 ccttgaggat ctcagccgcc gtgacgatga cttcgccgtc acattcgggg ctggagtcgc
    22981 ccaggatttc aatgcccccc tgcaggaaat ccttgccata cgtcgaccgg ccgtagtatt
    23041 cgcggaagac tgtactgaca taaccgaact tgagcagctg gttcgagcgc gggaattcgc
    23101 gggctgccgt ttcgacgaga ggcagcgtca catccggccg caggaccagg acacggccat
    23161 cggtatcgat ggtcttgacc atctgctggc ggagctgtgg aaagaagtgc tggtatacat
    23221 cgtaatcttc aaaactcggc gtttcaataa tcttacagcc gtggttcata atgacagaaa
    23281 cgattttccc ctggatccgg tcataattgc gcatttcatc atggtggagc atcttcactc
    23341 catccaaggt attgttgttc atcctatgac ctccctttct ttagttcttt attctagtaa
    23401 agcattaaaa tattggctgt tgaatattgt accacaaagt cctaaaaata aaaatacctt
    23461 tttctaactt tttacgcttt ttcactttca tcaccagcgc attttaatcg tttctttaca
    23521 gacttttcaa attttggcct gggaatcatg acccggtggc cgcacccggt gcagcggatg
    23581 acgaaatcga cgccgatacg cagcacttcc cattcattgc agccgcaggg atgggccttc
    23641 ttcatctgaa cgacatcacc tacataataa cggacaaaat cagccatcaa aattcctcct
    23701 tcaaaagaca aatagagtta tatatcatta tatagcccca ggcccccctt cgcaagggaa
    23761 cgggccgccg tgattgacct gctatagtgc ctgtgttaag ataacaggta atgaattgaa
    23821 gaggaggaat tgctttgaaa tcatcgatga gccggcagag cctgcgccgg tcctatctca
    23881 gctgttacat catcggcacg atctgcacgg tctgcgccat cgcgctcctg gcctgggtgg
    23941 ccatctgcat cgccctggaa aaagagcccc tggcatcggt cgcctttttg ccggacatgc
    24001 cggtatttgc catcttcatc gtcatcatcg ccatcgccgc catcagcgtc gctgcctggc
    24061 agtacggggc gacgtttcac cagcgctatg aagcactcac gggaaaggaa gggcctgaca
    24121 gagtaaagca tgagaaagaa tgaatttatg gacctcttgc gctactattt tcgcaagagc
    24181 gacaaaaatg atttaaaagg catcatcgaa gactgcgaag aacagttccg cctcggtgcc
    24241 aaagaaggca ggtcggaaga agaaatctgc tgtaaactgg gacatcccaa gaacatttac
    24301 cgctattata tcggcgaacc catcgtcccc gaagacaacc cgcgtatgcc cggcaacgaa
    24361 tacggcgcct acggcgattt cgggccgggc gtggaagacc gcgtcttcta tgaccaagac
    24421 cgggacgacg aatactactg tcagcagcag ctggcccagc agcagcgccg ttacgaccgg
    24481 gaacaggtca tgcgccagcg ctacgccgcc gaagccgcca agacgggcaa ggacttcaac
    24541 tggaacgacg gcgacagcca gatgggcaaa gcggccaaag ccgtcgccag ccccttcatg
    24601 gacgtcctgg ggaccctctt cggtatcctc agcggtttcc tttacctggc cctggccatc
    24661 gtcatcctgg cagccatcgg catttccttc atcccggcct atgcctatac ggacctcctg
    24721 cccttgccga cgctctccct ggtgacgcgg gtcctcgtcg tcctgacggt cctcttcgcc
    24781 gccatgacgg ccagctcggc cagccaggcc tgccacgccg cagcccgcgg gaagggggcg
    24841 cagtaaatga agcgcgtcct catcttcctc atcctgacac tggcctgcgc cttcggcagc
    24901 tacacatcct tcatgagcaa cgattacggc ccgttgaagc attggatggc ctattataaa
    24961 gagtataaag aaatccaggg tgccttggat aagggaaatg ccagcaaggc cgaactcctg
    25021 gctaaactga aggccatgta cccggaaaaa gcggaccaga ttgaagccca gttcaaggcg
    25081 aaatacggca gcctcaagga ctcggccgaa gatgccggcg acacggtcaa atcgaagtgg
    25141 gattcctata cccgcgacga cagcaccagc cacagccgct cccaatccca gagccgcagc
    25201 agccagtcgt cgtcttcccg gagcagccag agccagaaca gccgcagcag cagtcagaac
    25261 agcagccatg ctggcgaccg ggaccatacg gcagacggcc acgacaacaa ccgggcccgt
    25321 taaaagtttt ctactaacga ataataatta tccgttgaca tcagagttaa aaagtgctac
    25381 aatgaaatca acccaaagag gtgctaaaaa ataatttcaa atatgacttt tttatcaagg
    25441 aggaaatgaa ttatggaatt aaaaggttcg aaaacagaaa aaaacttaca ggctgccttt
    25501 gcaggtgaat cccaggcccg caacaagtac acctattatg ccagcgtagc taaaaaagca
    25561 ggctacgaac agat
//
LOCUS       DAWABZ010000012        25433 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_559_length_25433_cov_2.632319, whole genome shotgun sequence.
ACCESSION   DAWABZ010000012 DAWABZ010000000
VERSION     DAWABZ010000012.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 25433)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 25433)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..25433
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_559_length_25433_cov_2.632319"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1546..1555
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5124 a         6832 c         7412 g         6055 t
ORIGIN      
        1 cgtgggagcc gggctggttc ttgacgtgga accagaggtc atagggatgg gctttcttca
       61 gggtcaggaa gtcattctgc cggttgttgc ggccgaccca gatatcgagg ccgtcggcct
      121 ggagggtcag gatatcctgg gccgattctt ttttgagctt gtcccggttg tggccgctga
      181 tgaggttcat ctgtttcatt tcggccttga tatcggcgat ctcatctttc gtactcgtgt
      241 tttccagggc gtattccagc gaatagagat agtccaggtg tttctggttc tcttcttggt
      301 gtagcggttg taatagcgat tggcattgtc cgtcatggaa taggccggat tcaagggaat
      361 ggtcaccttt tcctggtctt ccgacaggag gttctggaca gtcacttctt tttcatggtc
      421 gtgcttttcg taggcgtaga tcatgagcag gtcaccgtag agcttgtacg tatccatttt
      481 cttcgtttct ttcatttcgc tggcaatgcg cttgatcttg cgcttctgct tttcgatgag
      541 cttgccgacc ttcttctgca gctgttcctg ttcgccgttg aggctgcggt gcgtggcctg
      601 gtattcgctg aggtaggtct cgatgaggct gaagtggcgc cacggatatt gggccaggct
      661 gtgcagttcc agcgggaaga tgacttcctt gcccttgacc gtatagacgt aagccccgga
      721 cataccttcc aggcgcttgc caaaggcttc gactgtacgg caccaggcga aacggtctgc
      781 cggcgacagg tccccgacgg gccgccctgc atcgatgccc gaatcgtggg acagttcgtt
      841 gaggacgatg gtgctcatgc cgttgaagcg cttgagcatc cacgtcttga gcggttcgtc
      901 cgtacccgtt tccatcatgt ccgtcagttc ctgggccgaa aaggcaaagg gatccatgcg
      961 catgaagttc ggcgggaagg catagggttc cttgggggcg atggtccgca gggcctgctt
     1021 gttcttcccc gtcttgatga gggcttcgat gatggtgccg tcttcggtaa agatgacgtt
     1081 gctgtatttg cccatgagtt cgacatggat tttacgtgtc accaggcggc ccgacatgtc
     1141 gaggacgtcg acggcgattt cgatgagccg gtccaggtgc agctgggcga tggacgcgat
     1201 gcggccgttt tcataatatt tgcgcaggaa catgcagagg cccgtcggga tatccggcgt
     1261 cggcggcatg gtatcggcga cgtacaggcg cggcgagtcg tccagggtga tgaccaggtg
     1321 atggatgccc gtatcgttga agatgcggaa atagagggac cgggcgtcga gctgatagat
     1381 tttagaaatc tggccgccct tgaggatgtc ggccagttcg ttggtaatgc aatgtaatgt
     1441 gataccatcc agattcatga tgatacctcc ttatcgtaat tctcactgaa acaaacaatc
     1501 cttttaatga taccatgttt tgccgtatcc tgtgaagagg gacgtnnnnn nnnnntcttt
     1561 taacagctag ccgctgactg ccaaaaagga gctgtcttca tgggacagct cctttttttc
     1621 actgcttcaa ataccagctc aagggctggg ctttggcggc ggggttattt tttttgttgg
     1681 ctgctggcgg cagcgaagat gagggaatcg cggtacatct tggctacggt ttccagggtt
     1741 tccaggcggt agaaggcctg ttccaggtct cggccgacgg tggcggcgcc gtggcgttcc
     1801 atgaggaaga cgtcggctcc ttctgcggct tcggcacagg ccgtggccag ttctgccgat
     1861 cccggcggtg cataaggtac ggtcggtacc gggcccagga ccaatgcccc ttcggtgacg
     1921 atgtccggcc ggaagggtat gccggctacg gtcagggccg tggccgtgac gggatgggcg
     1981 tggatgatgg cccggacatc attccgcttc cgataaattt ctaaatggag agccgattcg
     2041 gatgacgcct ttcccgtccc ttccaggaca tggccatcga ggtcgacgac gagcaggtgc
     2101 ttcggcttca attccccttt tggcacgccg gacggggtaa tgacgacggt accgtcgtcg
     2161 cggcggaaac tgatattgcc atcacaggcg gcgactagtc cggcttcttt cattttctgt
     2221 ccgatgcggc acagtgtttt cttggtatcc aagctctcgc ccccttagcg catggacggc
     2281 atattgcggc cgttgtagat ttccagcatt tcctggcgta cctggcggcg tatctgctgc
     2341 tgctggcgcg gcgtcaggtc atccttggta atattgaaga gataattatc caggtcgaag
     2401 tcgtgcagga ccatcttggt atggaagatg ttttcctgat agacattgac gtcgatcata
     2461 tcgtagcggt tgcgcgtggc cggggcgata taattctgga tggaattgat gcggtggtcg
     2521 atgaacagtt tctttccgtt cacatcgcgc gtgaagccgc ggacgcggta atcgagggtg
     2581 acgatgtccg aatcgaagct gtcgaggaga taattcaagg cgttgagcgg cgaaatacgg
     2641 ccgcacgtag acacgtcgat atcggcccgg aaagtcatga tgcccttctg gggatggctt
     2701 tcgggataag tatggacagt cagatggctc ttgtcgaggt gacagacgac atcggcatcg
     2761 tgcacttctt cttcggaaat gaggatggta acgctggccc cctgggggtc ataatcctgg
     2821 ctggcgacgt tgaggacgtg ggcgccgatg atgtccgcca cgttgcgcag gatctgggtc
     2881 agcctttccg cattatactg ttcatcgatg tattcgatgt attgacggcg cgactcaggg
     2941 ctgacggcat aacaaatatc atacatgttg aagctcaggg tcttggtcag gttattgaac
     3001 ccgtaaagct tgagtttatt cgttttcatg aatcttatca tccatttctg ctggaattac
     3061 cgttcgattt gatgaaaggc cacgtcggta atgacgccgt catcgatgga cacgatggca
     3121 tacgtcccct cccggccatc ccggggcagg ccgatactgc cgggattgac aacccacatc
     3181 tctccgtcca ttttggcgaa acggcggtgc gtatggccaa agacgacgag gtccgcctga
     3241 ttctgacggc ccagttcgac cagcttctgc caacgccgtt cgccatacca ctgacaaccg
     3301 tggatggcga cgatgcgaac gccgccgacg gtgacgcagc ggcattctgg atcatgggta
     3361 tgagtcatat aatcattgtt gcccaatacg gcatagacgg gaacccccgt ataaatagcc
     3421 aggtcttcgc catcatcaca gtagtcgccg cagtgaatcc aagccgccac gtcaggcgcg
     3481 tcatcggcca tgcgtgacag acattcaaaa cgaccatggc tgtcactgac caggccgacg
     3541 cggaccatac tcatgacaag gcctcgacga gcttcttcaa ggctttgccg cggtggctga
     3601 tggcgttctt ctcttccatg gtcatttcag ccatggtctt tcccttttca ggcacgtaga
     3661 agagcgggtc atagccgaaa ccattgtcgc cggcatagaa gtcgcgcagg acgccgtcac
     3721 agctcccttc agccgtcacg atccggccgt caggccagat gaggacgagg gaacagatgt
     3781 agtggaccgt ccactgttcc ggcggaaagg cggccaagtc ggcaatgagc ttctgattat
     3841 tggcttcatc gtcgcagtcc aagccggcat agcgcgccga atagacgccg ggccggccgc
     3901 cgagggcgtc agcggcaatg cccgaatcgt cggccaggca gggcaggccg gtggccttca
     3961 tgtaatacgt cgctttgagc agggcgtttt cggcgaaggt tttccccgtt tcttccggtt
     4021 cagtaatatc ggccatgacg tcgtggacgg aaactgcttc ataacctaac ggttccaaga
     4081 tgctcttgaa ctcccggatt tttccggcat tatgcgttgc cagtacgatt ttttctgcca
     4141 caggcctgtc tccttagtcc ttcttccggt tcttgacaaa ctgggcgtaa ttcttgataa
     4201 cgacgatagg tgtcttctga gaagcattgc cgaaggggtt gtcgatgagg attttggcga
     4261 tttccttcgg gttcaggcct ttgctgtggc cgaggacagc cgagcgcttg aggtcgttga
     4321 cgtcagcgac gacggcgccg tagcagccca tgcgctgttt gatcttttca gcgactttat
     4381 ccggttcggc cgggccgtag acgatgcatt tatcaaaagg cggcatggtg ccggtgacat
     4441 cgtccgtgag cgatgcctgg cggcatttgg cataccatac accgttcttg ccgacgagct
     4501 tggccaagaa gccgatgaag aaccagaaca tcatcttcca ttcgccttcg aggttcatcg
     4561 cagcctgcat gccatagagg ctggccatac tgccttcgcc cgggacgaag cggcgcatga
     4621 ggcgggcctg ccacgacggc gtcagttctt ccggacgggt atagcggcgc tgggtaatgg
     4681 cgacgacgct ttcggccgag cagaccaaat cgtccgggcc gacgatatct tttgcatatt
     4741 cttcaatgac gtcgacaata tcatctttat ccgtaaggat tctcgttttt acaggtacta
     4801 attctaattc tgccatctcg ttaaaacctc ctagttctgt tccaatgcgt gctgtacttc
     4861 cgaagccttc agggtgatgc gggctttatg gatgtaccat tcgctgcggg aaacgacctg
     4921 gtataccaaa tcgatgggca tgtcagggaa ggtcttcagg tcttcgcgga tattgccgtc
     4981 tttgctgacc agggcgatag tgacgcggat catgcccttg tcgccagggt tgaagatgac
     5041 cgattcccaa tagttgtcat cgcgttcagc cgctttattg gccagctggg aatggacgat
     5101 gaccttgtcg tactgttcgc ggggcagcag ggtccgggga tagaagtcca tgatcgtacc
     5161 gagctgacgg ccggcgttgt ggagggggac ttccgtttca aagacagccg tatcctgggt
     5221 catctgtttc agttcaaacg gtttgcggcg acgggcttcg atgacgaagc gggcatcgcc
     5281 ctgccgccaa gcaaagaggc ggaataaaag gtacaagaca gcgacaatgc cgatgacggc
     5341 aatgacaaca ttgagtaaga catagaaaaa cacgacgcaa gactcctttt atataatatt
     5401 ttgataacca ttaagtgagg gaaatttctt cagctgtaaa ttcattggcc gggatgaggt
     5461 gctgggccag gcgggagccg cggccgctct ctgcggtaaa gcacaggcgg agagtccccg
     5521 gttccggctg cttgttgagg aggccatcct ttttcaggac gtccaatgtt tccagggcca
     5581 tttcatgagc cgggtcgaca aaagcgatgg aatcaccgca gagttcttca aacaagggct
     5641 ggacgaaggg gaaatgggta cagccgaaga tgcccgtatc ggcaccggaa tgaaggaccg
     5701 gttccgtata gcgcttcaaa ggctcgcgga tttccgggcc gtcgaggacg ccccgttcga
     5761 tgagattggc cagggccggg caggactgct cgacgatttc gatgtccggg tccatggcgg
     5821 cggcgacttt gcgatggctg tggttggcaa tcgtgagcgg cgtggcaaag acggcaatct
     5881 tcttggtcgc gctgatttcc cgcgctgtcc gcaggccccg gctcatgccg atgaggggat
     5941 acggtacttc ctggacgacc gtatcatagg cgacggccgt cacggtgttg cagccgatgg
     6001 cgacgagctt gacgccctgg gccgtcatga agcgcaggat atcccgcgta taggtcagga
     6061 tttcttccgg cgtccgcgtc ccatagggat tgcgcttggt atcgcccacg tagatgatgt
     6121 tttcgtgagg cagcagcatc cgcagcttgg cgacgacggt caggccgccg acgccggaat
     6181 caaagacgcc gataggctgt tgattacgtt ccatggaccc ctcccccggc ctgggcagcc
     6241 gctttatctt cggcatacat ggcctgtaat ttcttataat cggcttcatc gaggcgggcg
     6301 actttgccgc tgtccttatg ggcaaagacg ttctgcgtcg tccccgtcgc cagcaggcgg
     6361 tggtcatcgt ggttgacgat gcggtacgaa aagaccatct gcgcccggct cagcttgacc
     6421 agccgcgtct cgatgtcgat ggtatcggca tagttgatgg gactgatgta ctcgcaggac
     6481 acgctcttga tgggatagga aataccccgg tccagcaggg cgaacaaatc gatgccggca
     6541 gctgccagat attcacagcg gccgcactcg aaccagcgga tatgattgga atggtgggca
     6601 atgcccatca tgtcggtttc atagaaccgc actttttcaa cgacagtaat cataagttct
     6661 taactcctaa ctatatgcat cttacatcat gcacactcat actattttac gatgaggaac
     6721 tatgtttgtc aaaggaaaaa gaggctgtcc cataaagaca gccgcttttt gagtattaag
     6781 tttgcagttt gtagttttta gtaaatattt ttaaatttaa agccatcttc tacaaactac
     6841 aaactcccta ctacaaacga taagtaaatg gaccgtgcat gatggcaaag cccttcagac
     6901 ggctttcccc gccttcacac gcggacgcgg aagaacggga acatatgacg tactttttca
     6961 cggtgttccg gcttgacgta agtcccggcc aggcgcaggg ccgcagcgac gacggcggca
     7021 tcatcgagcc agccgaagcc ggcaaagaca tcgggcacga aatcaaaggg cagaatgaca
     7081 taccccaagg cccccatgag ggccaatttg atgaatttcg gcgtgtcttc atcctgcata
     7141 cagtaaaaaa tcgtcaggag cttcggcaaa aagtggatct tcctccctgt cttgtggaca
     7201 aaggtgacga atttgccagg tgtataaaaa cgtgcaaaaa agcgcagtac cttccacatg
     7261 ggccttcacc ttacttctta tcttccaaag ggattttggc atcttctacg gcggctttca
     7321 ccttgtcttt gacagacgcc gtatcgccgt ctgcagccgc tgccgtttcc ggttcggcag
     7381 ctgccgtcgt ttcagccgag gcttcggctg tttccacagt ttcggcagtt tctgccgttc
     7441 cttctgcctt ttcggcttca ccggccttgg tttcttcgac cttttcttct acgatttcat
     7501 cgaccaggtc gtcgtgatgg cggacggtgt gatagagttc ttcccctttt tccttgacgt
     7561 cctggaagcg gtccttggcg acgccgcccc atttcttcat gaagtcgatg gtttcatcgc
     7621 cgagggggac gttcttcaag ggattttcct tgagtttgtc gacttgttcc cgagccttat
     7681 ccttccattc gcccatcatg tcgacgtagg gacccatgtc ggtaggcatg ccgtccttga
     7741 gccagcgctg ggtgacgcgg cccaggctgt aagtgatgcc gacggcgaca ggtacctgga
     7801 tggcgctgaa aggaataatc gtcgtcagca ggctgccggc gacgcggctg ccgacggcgc
     7861 cgaggaaggc gatgagggcc cgttcgctca gtttgacatc atagaccttg gcaatgcggc
     7921 tgaccagata gacttcgttg gccatgaggg cgacggtccc gacgagcggc gcaatgacga
     7981 tggcaccggc ccgggctgca gcccagcggc acaacatttc gactttctcg tctttgtctt
     8041 ctgtcgactc ttccatggcc ttgacgacac tggctgcggc atcgtcggcc ggctgggact
     8101 gggccttcat gtcggccagt tcctgtttca atgcggcgat ttctgcctgc agttcttcta
     8161 cagtcttggc ttcttctttg tatacttctt ctgccataac aaacacccca ttctttaacg
     8221 tattaaaatt tatactctat attttcccat gtttctacca gcttgtcaaa cccccatcga
     8281 caggaagtgc cacgccggta atgaacgacg cgtcatcgct caagagaaag gcgatggcct
     8341 tggccacttc atcgggccgg gccagtcggt acaagggata ctgctctttc atggcatccc
     8401 gcgtcgtatc gggatggtcc cggagctgct gctccagcat aggcgtttcc acgtcaccgg
     8461 gacagacgca gttgacccgt atctggtgcg gtgccgcttc cagggccagg gactgggtga
     8521 aggccacgac ggcgcctttc gtcgccccgt agagagcgca ggcgatgttg ccctggagac
     8581 cggcgtcgct gctgacggtg acgatactgc ccggccggcg ccgcaagtgc ggcagggcat
     8641 agcggcagag cgagattgtc ccgaagacat tgatggaaaa gaggcgctgc atgtcggcag
     8701 gcgtgacatt ttccaagagg ccttgttcat agacgccggc cgacgtgacc aaaccgtcga
     8761 ggccgccgaa gtgagaaacc gtctcttcga ccatccggcg gcagtcgtcg tcgcaggcca
     8821 cgtcgccctg gatataccag gtccggtccg gcgcagacaa cgccgccagg gcggcctccc
     8881 ctttttcccg atagcggccg ctgatggcga cgttccagcc cctttccagt aagaccgccg
     8941 ctgccgccag gccgatgccc gacgtgccgc cgctgatgag taccgtatcc atagcgtccc
     9001 tcctacataa taccaaaata agataagccc aatttgccga tgaggacgac tgtgacggcc
     9061 agcatgacca ggcggacgaa gcccgtaccc cggcggatag ccaggcggga accgaagaag
     9121 gcgccggcaa agatgcacag ggccatggcg atgccgtaga cgtagaggat ctggccccac
     9181 cagatgagga ggacgaagga cgtcacattg ctcatgaggt tgaggatctt ggcattgccg
     9241 gcagccgtga cgaagtcgaa gcccaaggcc gcaaagccga cgctgaggaa ggtccccgtc
     9301 tccggcccga tgaagccgtc atagaaaccg atggccgagg ccatgaccag ggcggccagg
     9361 acgttcttcc gggtattgcc gctgtacgtg ctggcagcgc cgagctgacg ctggctgaag
     9421 acgaagaccg ctgccgcgat gagggcgatg atgatgatgg gctggagcca ttcgctcttc
     9481 aagtgcagca tgaccaggca acccaggatg gcgccgatga aggtccccgg caggatccgc
     9541 ttcaagaggc tcatgtcgac cttgccagcc cgccagaact gccaggcgct catgacggcg
     9601 ccaaaggtcg cggaaaattt attagtccca atggccaggt gcggcggcag gccggctgcc
     9661 aaaaggacgg gcagcgagat gaggccgcca ccgccggcga tggaatcgac gaaaccggcc
     9721 aggaagccgc cggccatgat gaagagcagc agcgacgccg acagggtcgt atgaaaaagt
     9781 tggaattcca tgaaaaaatt actccttttg gaaaaaaact cctcttgctt cgcagagcga
     9841 aaccgaggag acccttttct cagtgttcat atatcaagtc cgccgccgtt cccgctgtct
     9901 ggggccgcag aaggcggacg atttccttgc cgatgatccg cgcaccgtcg cggtggcagt
     9961 cgaggctggc ttcggacatg gctgccagct tatcgggatg tgtcaacagg tccgctacga
    10021 cggaaacgag gtcttcctgc ttcttgaccc agcgggcaca gcctttggcc tgcatgtacg
    10081 tcgcattggc ttcttcctgg cccgggatgg gactataaag gaccatcggt acctggacgg
    10141 cggccgcttc ggtacaggtc agggcgcctg gtttggtgac cagcagcgat gcctggcaca
    10201 tgagctgggg aatccggttg gtatagccca ggatgttgac gtcgtgatgc agttcgctgc
    10261 gcatcttgga cagttcgtca tagagatcgg cattgtagcc ggtgacgaca gtgaagctgt
    10321 cgacggcgtc gaggcggtcg aggagcgtca gggactgttt gatggaaccc atgcccaggc
    10381 cgccgcccat gatgaggacg ttcttatccg ccttatgggc ggcatcccaa tgccagcgtt
    10441 cctgttcaaa ggccttgcgc acggggatcc cggagacgac gatcttgccg gcatcgatgc
    10501 cctgttcgac gagcaggtcc ttgagctggg acgcggcgac gcagtagaca tcgatctgag
    10561 gatagaccca gagctgatgg atggcaaaat cggtgatgac gccgaccagg gggatatcga
    10621 ggcggtgctg gcgcttgagg agcgctgccg cccctgccgg gaagggatgg gtaaagacga
    10681 ggacatcggg cttcttttcg gccaataccc tgagcatccg ccgcttgagg ccccaggcaa
    10741 agagcgtctt gacgacgagt cccttcttat atccttgtga cgaatagtac atcaaatcgt
    10801 acagcatggg aaagacatcg aggatcttga tgtacgtttc cttgacgatg ttgtcgatag
    10861 acaggacgtc gttggacagg aaatccaggt gttccacttc acattcttcg ggaatggtct
    10921 tgacgtattc ttctatcgcc cgtgctgcct gtgtatgccc cgtaccgatg gatgcggaca
    10981 ggatcaggac tttcttcttt gtcatgcatg ataccctctt ttataaacag cattagtcta
    11041 gtagctcata ctactatggt acgacaattt gcaagaaaag aaaagggccg tttttcatgc
    11101 ggcccttcgg aatcgtacct tatttgttgt tgaccaaatc tttgagctgt ttgccagctt
    11161 tgaaagccgg ggtcttggat gcttcgatct gaatcggttc gttgttgcgc gggttgcggc
    11221 cttcacgggc tttacgctga cggacttcaa aagtgccaaa gccgatgatc tgtactttat
    11281 caccctgggt gaggctgtcg ctgatagctt cgaatacagc ttttacagct ttttcagcgt
    11341 ctttcttggt caaatcggat ttctgtgcga ctgcagcgat taattctgtt ttgttcatga
    11401 tactaggtcc tcctttaaat cgttacatct gcgtaacgct aactgtctgt attttccttc
    11461 tgccgttcta tctgtttggc ctcctgccag aacgagtcga gctcatccag cgaaaaagca
    11521 tcccacggcc ggcccgactg gcggacccgg tcctctacgt gagcaaagcg gcgtcggaat
    11581 ttacagttag cccgatgtaa cgcacattcc ggctctatat gatagtgtcg gcataaattt
    11641 gcaaagacaa acaggacatc cccagcttct tcttcggcgt ggacggaatc tccttcgcca
    11701 agggcctgac ggaattcctc ccattcttca tggaacttat cccagacgcc ctggacatcg
    11761 ggccagtcga aaccggtctt agccgctttt tcctgaagtt tatctgcagc caggagcgac
    11821 ggcagtccgg gaacgacgcc gtccagcagg tgagcatgct gctggcgttg ttcgccttgc
    11881 tttaatctat cccagtttaa catactcgta cctatgtttt caaggctttt ttggctaaaa
    11941 acgtagggat ggcgccgaat catcttatct gtgacgtctt ttacgacatc ttgagccgaa
    12001 aaaaggcctt tttcctgggc gatgcgggcg tggatggcga cctgatagag gatgtccccc
    12061 aattcctcgc ggatgcccgc tacgtcgtgg ctgtcgatgg catcgagcat ttcatagacc
    12121 tcttcgatga ggtagcggcg caaggtccga tgggtctggg cccggtccca ggggcagccg
    12181 tcttcgcggc gcaggcgcgc cgtcacgtcg acgatgggcg ttacgtcgaa gggcatggcc
    12241 tcagcaccgt ccccctgccg cttccgggcc tcttcgatga gacggcacac ggcctgggcc
    12301 atcgccggtc cacccgggac ggtatggacg acggcatgga ggcggctttc gcgcagcagc
    12361 agctcaacga tgggcgcata agtctcggca aaagactggc cgtcctggcc atacgaatcc
    12421 aggggcttgt cgtcgctccc cagcgggatg atcttgattt ccatagcagt tcctcatttc
    12481 ccggcaaata tgcctatgcc tgtcattata acacggagtc cgtcatttgt cgcggcctgg
    12541 caggaaaaag ggcgtcccac agggcctggc ggacatacgg gccataggtt cccttgagta
    12601 tcgcccccgg cccctgacag gccgccgtga cgatatctga taggattcgg gcctgggcag
    12661 ctgaaaaagc gctatgcctg ccggccaatc catgcagatt ccggagggta aaggccgccc
    12721 gttcatagtc ttccttgacg atgagccagc gcacctggaa cagggcctgt acatcgcggt
    12781 gaaagcggcg caggccgccg cagcggtccc accagtcata gaaccgctcc tcctgtctgc
    12841 ggcgcaggct caggcgcagc agggccacag acgggcgcag atagtgctgg cggtcatacg
    12901 tgccgggagg ctgccgctgc tggagctgcc gggcccggtc gtaatagccc tcggcctggt
    12961 cataggccca tcgggggcgg ctggtcagcg gccattgacc gacccgttcc aggaggacgc
    13021 ccagaagata gggatatacc ggctggtccg gctgctgtcg gcaggccgcg agcagttccc
    13081 ggaccatacg gcggcagcgg cagatccggc cggcctggcc cttcgcctgc cccaggggca
    13141 ggccatagag cagagagatt tcttccaaca gagatgagcg cagggacaag taatggaagt
    13201 cctggcggaa gtgatgagtc ggatacatgg gcaaaccttc tttctttttc catttgcccc
    13261 cagtgtaaca gggaaaaatg aaaaaaacct gaaaagcagt gcttttcagg ttaaaatttg
    13321 ctgagaaaat catttctggc tcagtttcgt cagcaggcgg atgagttcgg cctgttcgtc
    13381 ctcactgagg cggttcatga gcgtcgaaat gcgcagataa tggccgggca gtacttggtc
    13441 catgaactgg cggccctggg ccgtgaggct gacctttttg ccccgcttgt cttcctgatc
    13501 gatgacgacc gtcgtcaatc cgtcgcggac catgcgcttg accatggtcg taatcgtcgc
    13561 ccgggtcacg ccggtcttct cagccagctg tgacggtgca ataccttccg ggctctggtg
    13621 caggacgatc atgacccgca gtttcccctg ggacaagtgg tattgcttgg ccaggacgtc
    13681 gttgatggac gagcggatga tttcagcggc ctgggagatg cgcagcatgg ccaggaccgc
    13741 cgacggttcg atttccggaa tgatccgggc gtgtttttcc aatttttccc gcgtcgggaa
    13801 tgcttcaaat ctcatacgtc ttcccctctt ttgattagct tgctaattaa aattataaga
    13861 taaaacgagg aaaaatacaa gcatttttcc tcgttttacg gaaatatttt tataacatat
    13921 gcgttcgatt tacatattct tgctacgcag gtcagctaag atttcctgga cgcgggcgac
    13981 ggcctggtcg ataggaacga cttcctgttc gctagtccgg cggatcttga tttcgacagt
    14041 gccttcggca gcgctcttct tgccgaccgt gacccggacg ggatagccga tgaggtcggc
    14101 gtcgttgaac ttgacgccag cccgttcatt acggtcgtcg aggaggacgt cggccttggc
    14161 agcctgcagg gcctgataga ggtcaccggc gatgcggacc tgatcctgat ctttgctgct
    14221 gaccgggacg atggccactt caaaaggcgc gatggcgaca ggccacatga tgccgtgttc
    14281 gtcgtggaac tgttcgatgg cagcggcaat agtacgggtg acgccaatgc cgtagcagcc
    14341 catgacgtag ggtttggcct tgccgttctg gtcaaggaag gtcgcatgga gggcttcgga
    14401 atacttcgtg cccagttcaa agacctggcc gacttcgatg ccgcggacga tttcgatggg
    14461 tgcaccgcag tgcgggcatt tgtcgccggc gaccatgttg cggatggtcg tgaccgtcac
    14521 gtctttgaag tcccgcgacg gattgacgtt ctgatagtga tagccggcct tattggcacc
    14581 ggttacgcca ttggggacgt tcatggccga cggatcgacg aggacgaaga acgtttcatt
    14641 atccggttta tcggcaccca tggggctcat gtagcccggc tggaggccgt gggccttcaa
    14701 ttcgtcttcc gtcgccggtt cgatgacatt gccgccgacg aaattctgga cggcgacgtc
    14761 attgacttca tggtcgccgc ggaccatgca gaggaccgtc ttttcgccgt cgatagtgaa
    14821 agcgacggct ttgatcgtct tttcgacggg aatgtggagg aactgggcca ggtcttcaat
    14881 ggtcgagcag ttcggcgttt cgatgagttc tacatctttg gcgtcttctg ccggcatttc
    14941 gatggcctga ggaacggctt tttcaatatc tgctgcataa tcgcacttcg agcacgatac
    15001 gatttcggct tcgccggcct gggccagggc catgaattca tgggtgtgat tgccgccgat
    15061 ctggccgctg tcggcttcga cgggcttgaa attcaggccg cagcgcgtga agatgcgcgt
    15121 ataggcatcg tattcatcct gatagctctt gtgcaggcct tcttcgtcga cgtcgaagga
    15181 ataagcatct ttcatgacga actcacggct gcgcatgagg ccgaaacgcg ggcggatttc
    15241 gtcgcggtat ttattctgca tctgccacag cgtgacgggc atctgcttgt acgagcgcag
    15301 ttcattgcgg accagggtcg taatcaattc ttcgtgtgtc ggcccgaggc agaaatgatt
    15361 gccatggcgg tcttcgagtt tgaacatttc cgggccatag gcattccagc ggcccgattc
    15421 atgccagatt tcagccggct gcataatggg catcatgatt tcctgggcgc cgatgttgtt
    15481 catttcttcg cggacgatgg cttcgatctt gagttcgacc cggcggccta aaggcaggaa
    15541 agaatacaag ccgccgccgt tcttgcggat catcccggct ttcaacatat actgatggct
    15601 ggcgatttcg gcgtctgccg ggatttcccg cagcgtcgga ctatacaatt tcgatgctaa
    15661 catgagttaa gactcctttg caatggtttt ttcaatttct tccagcaggc tggacacgag
    15721 ccggtcttcc ggcacggtgc gcaggacctg gccatgagaa aagacgatgc ccttcccctt
    15781 accgccggca atgccgaaat cggcttccct ggcttcaccg ggaccgttga cgacacaacc
    15841 catgacggcg accttgatgg gcacagtcag atgagaaatg gcctcttcca cttcctgggc
    15901 caggtcgatg aggtggacct gggtccggcc gcaagtcgga caggaaatga gggtcggccc
    15961 gaaacggcgc aggccaaggg cactgaggat ggtctggccg gccttgattt cttcgacggg
    16021 atcgcccgtc agggatacgc ggatcgtatc gccgatgcct tcgtggagca gcgtaccgat
    16081 accgatggcc gacttgatgc tcccttcacg gaccaggccg gcttcagtta cgcccaagtg
    16141 cagggcataa ggcaccttat cggccagggc ttcataggct tcgatcatca agggcacgtc
    16201 ggaggacttg agggaaatga ccatgttgcg gtaatccatg cgttccagga tgcggatatg
    16261 gcgcagggcc ccttcgacca tgccttctgc cgtcggatgg ccgccgctct gagccaggat
    16321 atcttccggc agggaacctg tattgatgcc gatacggatg gggattccct taggacgtac
    16381 tttttcgacg actttgacga cattgtcgtc agatccgata ttgcccggat tgatgcgcag
    16441 ggcctggacg ccgctgtcga cggcggccag ggccaggcgg taatcgaagt ggatatcggc
    16501 gacaatgggg atatccacgg cctgaacgac ggcggccagg cctttggccg cttccatatc
    16561 gggaacggcg aagcgcaaga tttcagcgcc atacgatgcc aggcggcggg catcgacgac
    16621 ggcttcggcc atatgggccg ggttgaccgt cgacatggtc tggaccgata cgggtgcatc
    16681 gccgccgatg gtgacgctgc ccgcctggac gggaagagat tttctacgtt tcatgcaagg
    16741 cctgcctttc taaaagcggc tgatatcctg aaacatggcg aagaggaaca gggagcccaa
    16801 gaggacggcc cctgtgacct ggatgtagta caacgcccgt ttcggcatcc gccggcgcgt
    16861 aataccttcg agaatcaata aaataatata gcccccatcg aggagcggta cgggcaggag
    16921 attcaagatg cccaggttga ggctgaggaa ggccgtgaac atgagcaaat tggcaaagcc
    16981 cgatgcagcg acctgaccgg ccagctgagc gacgccgata ggaccggcga cttcggcctt
    17041 ttcccggccc gtcaccatat catagaggcc gacgatcatg agctggcaga gcttgcctgt
    17101 ccgttggacg gccatgcccg ccgattcaaa gaagccatga gcttctttgg tcgtcatggg
    17161 catgacgccg agcatggctt tcttcgaggc gctgtctacg tcaggcacca tttcgacgcc
    17221 ctggatctgg ccgtcccgtt cgatttccac ggtaacgacg gcgtggctgt atggcgcgac
    17281 ggcctgagaa atatcagacc atttggaaat ttcctggttg ccgatgcgga cgatgcggtc
    17341 gccggctgca aggcccgact gttcggccgg tgagccggcg atggtcgagc cgacgacggc
    17401 ttccggcgaa acggtcaccg taccgacgac gaagaacagg ccccacaaga ggacgatggc
    17461 caggacgaag ttcatgatac cgccggcagc gatgacgatc atgcgcttcc agacaggttt
    17521 gctcaggaaa gatttatcgt tcaagtcttc ctcttccgtc atgccggcga tgcggttgaa
    17581 tccgccaaga ggaatggccc gcagggaata ttctgtatct ccgcggcgta cactgtacac
    17641 tttgggacca aagccgagtg cgaactcatc gacctgcatc cccgcccact tggccgtcgc
    17701 gaaatggccc agttcgtgga cgaagacgat gacactgaag acgaagatcg tcgctaaaat
    17761 agtaagaccc acgggaaatc ccctttctac agcaaggaag cagcacagcg gcgggcccag
    17821 gcatcggcct ggcggataga cgccaggggg acgtcatgcg agaccgtgtg ccgttccatg
    17881 actgtacgga caatatcaaa aatacgcgta aaggaaatct ctttacgcag aaaagcatag
    17941 acggcgactt cattggcccc gttgaggaca cagggcatgg taccgccgat gcggccggct
    18001 tcataggcca ggccgatgga tgggaacttc tcgtcgtcag gcgggaagaa ttccagcgtc
    18061 cccagcgtct tccagtcgat gggccgggcg ccgcggacgg tcatccgttc cggataggta
    18121 aaggcgtact ggatgggcag gcgcatatcg ggcatgccca gctgagccag gacagaaccg
    18181 tcgatgaact cgatcatgga atggatgatg ctctgcggct ggatgacgac ttcgatcttg
    18241 tcgaagggca tgtggaagag ccagtgggct tcgatgactt ccaggccctt attgaacatc
    18301 gtcgccgaat cgatggtgat cttgccaccc atgttccacg tcggatgctt gaggcactgt
    18361 tcgaccgtaa tcgacgaaaa ttccgacgca ggccggcgga aatggggtcc gccggaagcc
    18421 gtgaccagca gtttggccac ttcttccggg cggcgcccct ggaggcactg gaagatggcc
    18481 ccgtgctcgc tgtcgacggg caagatgcgg acgccgcggg ctgcggcttc cttcatgacc
    18541 agttcaccgg cagcgacgag ggtttccttg ttggccaggg cgatgtccat ccccttctcg
    18601 atggcgtgga gcgtcggttc gataccggcg gcgccgacga gggacgtcag gacgatctgg
    18661 gcgtcgcatt catcgatgag tttcaagagg ccgtcactgc cggaaaagac agtacacgcc
    18721 ggaccgacgg cacggcgcag ttcatcagcc gcctttttgt cgaccatgca ggcccatttc
    18781 acctgatggt cccggacctg gcgggccact tcctgccaat gggaataagc ggcgacgccg
    18841 gccaggcgga aggcctcggg atgggcgtcg atgacgtcga cggcctgggt cccgatagag
    18901 ccggtactgc ctaaaatcaa aatatccttc attccctgcc ctcctttaaa tgaccagcat
    18961 caaatagacg tacatagccg gcgctgcaaa gagcaggctg tcgaagcggt cgaggacgcc
    19021 gccgtggcca gggatgatgt tccccgaatc cttgacgccg cagacgcgct tcaagatgga
    19081 ttcgaccaga tcgcctaaag gagccatgat gccgatgatg acgccaatgg ccagggcgtg
    19141 gaggacggag aatttaaaga tgagcgaaaa gatgatggcc acggccaggg taccgacgaa
    19201 accgccgata gcaccttcgc gggtcttgcc ggggctgatg gccgggcaga gcttgacttt
    19261 gcccatcatc ttgccgacgg caaaggcaaa agtatcgctg gcccaggtcg aaaagatgag
    19321 gagaaagacg aagaaccgcg ccggttccaa catgacggcg ccgaagctgc tgcccatgaa
    19381 ggaagccaca gagccgctgc gcagggccag catggccaga aagccacagc cgatatagag
    19441 caggccgtac aaggtatagg cactgtctac gggcttaatc ttgttgtgga aaaagacggt
    19501 ccgcagcatg agccagaaaa ggaggcccaa ggccaggacg agcatgacct tggtgctgcc
    19561 gaaccaatag gcggccagca tggcgacgag ccatacggca ccgcacttgt cggccgtcac
    19621 ggcgtggagc ttgtggacca ggttgccgta ttcataccag gcgacgaggg ataacagggc
    19681 catcatgagg aaaaacagcc agttcccttc gtaaatcaca aaaatcgtaa aggcaccacc
    19741 gacgatgcct gtcagtattc gttttgccaa catatcatat accttccctg atttatttta
    19801 agccgccaaa gcgccgttcc cggccctgga aggacaaaat agcttcgacc agggtatctt
    19861 tgctgaaatc gggccagcag agctgggtgt accagaattc ggcgtaggcc atctgccaga
    19921 ggaggaaatt gctgacccgc ttgtccgcac cggggcggat gagcagatcg acgtcatttt
    19981 ccggctccgt atagagatac tgggccagcg tcgcttcgct aatgtcttcg gggcggatct
    20041 ggccgtcccg gacggcttct gccactttgc gggcagcctg ggtgatttcc aggcggccgc
    20101 cgtaattgac ggcgacgttg aggataaggc ccgtattggc ggccatttcg gcttcggcct
    20161 gagaaaaggt atcctgcagg tccggatgga gccgcttcat atcgccgatg acgtggagct
    20221 gcacgttgtg ttccttgagt tccaggagat tcttgctcag gtaactcttc atgagcttca
    20281 tgatgtacga cacttcttca tcagggcgct tccaattttc cgtagaaaag gcatagaccg
    20341 tcaggacctt gatgcccagg tcgtcggcag cgacgacgat gcgcttgagc gtatcggcac
    20401 cggcgcgatg gccgtaactg cggggcaggc ccttgcgctt ggcccagcgg ccattgccgt
    20461 ccatgatgat ggctacgtgg cggggaatgt tgttcggatc cagcatggct tcggacacgg
    20521 agcctgctgt ttccttcttt ttgccaaaaa agttaatcat gctgtacgtc tccatttcgc
    20581 agggctgccg ctacggatcc gctcatctcc aggctgtcgt tgaccaagag gcagactgtg
    20641 ccgtcatccc gccgctgggc gcggatgaca taggtctgct cagggtcgca gtggaaaaag
    20701 tggctccgcg acagcgtccg ctctatgaca aaaggaatat ttttttcctg taatacagat
    20761 ttggcatacc gtaacggcat gccaataaaa atccgtatat ccatcatacg gccatgactt
    20821 ctttttcttt tttcgctttg atttcgtcga tgaccttgat ttttttatcc gtcagcttct
    20881 ggatgttttc ctgaccttct ttgctgtcat cttccgtgac ttccttcttc ttttccatct
    20941 tcttgatggt ttcgttggcg tcgcggcgga tattgcggat gacgactttc gagtcttcgg
    21001 caatcttgct gactttcttg ctcagttcct tacggcgttc ttccgtcagc tgggggattt
    21061 ccaggcggat gatcttgccg tcgttgttcg ggttcaggcc caagtcggac ttgaggatgg
    21121 ccttttcgat ttcaccgagc atcttcggtt cccacggggc gatggtgatc atgcgcggtt
    21181 ccggagcggc cacattggcg acctgtttga ccggcgtcgg cgtaccatag tagtcgacgg
    21241 tgatccggtc cagcagcgac gtgctggcgc ggcccgtgcg gatcgacgac aattcccgtt
    21301 tcagggcttc gatggtcttg tccatctttc cttcgtgctg ggcaatgatt tcttttactt
    21361 ccatgtcaat cacttcctta ttttattttt ataagtatat catacagctt ttagtcttct
    21421 ttgtcgacta cagtgcctaa atcttctcca ctggcggctt tgacgatgtt ccccggctgg
    21481 tcgatgttga agacgacgat ggggatgttg ttgttgcggc agagcgtcgt agccgtagca
    21541 tccatgacct tcaggtcttt gttgatgacg tcgagatagg tcagatgctt gaacatcttc
    21601 gcatccggat tgagtttggg atcgctgtca tagacgccgt cgacgccgtt cttggccatg
    21661 aggatggcat cggcttcgat ttcagcagcc cgcagtgcgg ctgtcgtatc cgtcgagaag
    21721 taagggttgc ccgtaccggc accgaagatg acgacgcggc ctttttccat atggcggata
    21781 gcccggcggc ggatatacgg ttcagcgacc tgctgcatgt tgatagccgt ctggacgcgg
    21841 gtagcgacgc cgttcttttc catagcgtcc tggagggcca gggaattcat gaccgtagcc
    21901 agcatgccca tgtagtctgc cgagacgcgg tccatgcctt tagccgagcc ggacaggccg
    21961 cgccagatgt tgccgccgcc aacgacgatg gcgacctgga cgccgagctt attgacttcg
    22021 acgacctgct tggcaatggt ttcgaccgtt tccgggtcga tgccgtaacc ctgtttgcca
    22081 gccagtgctt cgccgctcaa tttcaatacg atgcgatgat actttttcga ttccataaaa
    22141 gaagtgcctc ctctgtcggt atacaagtcc aattagaaaa taagagaaca cgggttgggt
    22201 gttctcttat gcaatcttat ttagcttgtg ccattacttc ggaaacaaaa tcatcgttcc
    22261 gtttttccag gccttcaccc aattcataac gcgtataacg gcggatgtcg attttttcac
    22321 cgatcttagc gatggcttcc gtaacgacct gctggaccgt cttgtcagaa tctttgatga
    22381 attcctgatc gaggaggcag ttttctttgt agaatttttc gatacggccg acaatcatct
    22441 tttcgacgat cttttccggt ttgccttcgt tcagggcctg ctggcggaga acttcttttt
    22501 cgtgttccaa tacgtcagcc ggaacttctt cgcgtttgag gtatgccggt tttgcagcag
    22561 cgatctgcat tgcaatgtct ttgcagagtg ctttgaagtt atctgtctga gcgacgaagt
    22621 ccgtttcgca gttgacttcg acgagaacgc cgatacggcc gttgccatgg atgtaagaat
    22681 aaaccaaacc ttcagcagcg atgcggtctg ctttcttagc tgctgcagcc aagccgtgtt
    22741 cgcgaagcaa gtcgacggct ttttccatgt cgccgttgct ttcggtcaat gcttttttgc
    22801 agtccatcat gcctgcaccg gtttttgttc ttaattcttt taccattgct gctgtaatag
    22861 ccatgagtat tcctcctaaa agtaatgtga attatacagt ttgaagaaag gtaagaagca
    22921 aaggctcctt acctttctat aataaccatt cagttctcaa agaacgagta atcttattct
    22981 gctgcttttt cttcagcggc ttcagcacct tcagcggcgc cttcttcagt ctgttcgccc
    23041 tggttgcctt cgaggacagc gtcagcgatc ttgccggtca agagtttgac ggcgcggata
    23101 gcgtcatcgt tagccgggat cggatagtcg atgacatcgg ggtcgcagtg ggtgtcaacc
    23161 gtagcgatga tggggatgtg aagtttgcgg gcttcagaaa cagcgatttc ttcttttttc
    23221 gggtcgatga tgaagagagc gtccgggagt ttcttcatgt ctttgatgcc gccgaggtat
    23281 ttctgaagtt tttccatttc atgtttgagg agcgtaactt ctttcttcgg caagagttcg
    23341 aacgtgccat cttcggcctg tttttccaat gtcttcaagc gttttacgcg cttttcgatg
    23401 gtctggaagt tggtgagcat accgccgagc cagcgttcgt tgacgaagta ctggttgcag
    23461 cgttctgctt cgtttttaat ggattcctgg gcctgttttt tcgtgcctac gaagaggact
    23521 ttgccgcctt cagcagccag gtcgcggaca aaattgtaag cttcgtccat tttcttaacg
    23581 gttttctgca agtcgatgat gtagataccg ttacgttcgg tgaagatgaa acgagccatc
    23641 ttcgggttcc aacggcgggt ctgatgaccg aaatgaactc cggcttccaa taattgtttc
    23701 atagatacta ctgccataat tgtaattcct cctgtttttt cctccgcccg gtcatcgctc
    23761 atcttcaccc ttccccacgg ggaacgggca cagtgctgag ctcgccaagc gtgtgtattt
    23821 tagggtttgg tattcaaacc tttggtatta tagcatatat gacaaacaag ggcaagcatt
    23881 ttttcaaatg cctgcccttg ctttgagttg taagcttgca gttcgtagcg gataatgaaa
    23941 aatttaattc aggacataga ccggtacggt gtggcggccg aagttcatgc aggcatcgta
    24001 gtcttccatg cagaggtcga tgcggttgcc gaggatggct ccgcctgtat cttcggcccg
    24061 ggctacgcca tagccgggaa tgaagaccgt cgagcccagg ggaatgacgt tggggtcgac
    24121 agcgacgacg ccataacggg cccgcgtccc catgcgggtg atgcactggc cgtcgccgtc
    24181 cgtcggcaga taggccgtcg cttccatggt gatgatcttc tggtagtggg atacatcacc
    24241 tgtgctggta ttgatttcaa cggtttcctt agtgccgact ttgcggacca gaggctgcat
    24301 ttccgaaatg agctccgtcg acagcgtttc cttgtcggcg acctggccgt cgacgctgac
    24361 gatgttcagc ttgacccgct tgcggccgtt ctgcccttcc tggatgactt tctcttcgcc
    24421 ctggcccaag gtttcatcag ggatgctttc gatggcatag ggcacgactt catcttccgt
    24481 gatggtcttg cgcgatacgt ggccgatgac gatgtgctgc cccgcctgga gcggtttctg
    24541 gacgtcgcca taagggcggt acttttcctt attatagccg tactgttcgg cgacttcctg
    24601 ggaattgtgc ttggccgtgt agacttcctg ggtctgtccg cggtactcca gggtgaccgg
    24661 gatggcccgg cgcaccttga cgtagctgcc gtcgtcgagt ttctgcgtcg aaacatcgat
    24721 ttcatcgtaa tcaccgatgt cgatattgtt ttcccgcatc aatgcctttt cattgacagc
    24781 gtgggtatat acctggcggg tttcgccatc ggcgacgagg gtcactgatt tccccctgtc
    24841 gatgaaccct gtcagggaca cgatgaccaa taaggcgatc atcgtcgcgt acatgcgtgt
    24901 aatcggtcgt ttattcttga atccagtcat gctgaatcgt ttccagtcca tgccatgcct
    24961 cctaatgctt ttgttttcat tgtagcaaag accggcaggc ttgtcaattt acttgtcttc
    25021 ttcagcagga cgcgggcgca ggacgacgtt gcggaactgg cgggctgcct tcttccgttc
    25081 cgatgccagc cgttcagctt cgcgctggca tgtatcctgg gcgttgacag acttgccctt
    25141 gcgttcgtct gccaggtagc ggtacgagcc gtcgctgttc atagcatagg ccttgacgtt
    25201 gtcgtcgaag tatactttca ggatgcgctt gatccgttct ttatgcggcg ggaattcgac
    25261 aggtgtcatc aattcgacgc gttcgttgag gttgcgcggc atccagtctg ccgaagaaat
    25321 gaagacgtct tccttgccgc cgttgtagaa gtagaacagg cggctgtgtt ccaggaagcg
    25381 gccgacgatg ctgtggactt cgatgttttc gctgatgcca ggcaggccag ggc
//
LOCUS       DAWABZ010000013        24557 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_592_length_24557_cov_3.288507, whole genome shotgun sequence.
ACCESSION   DAWABZ010000013 DAWABZ010000000
VERSION     DAWABZ010000013.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 24557)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 24557)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..24557
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_592_length_24557_cov_3.288507"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1411..1420
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10232..10241
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13774..13783
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17470..17479
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5589 a         6748 c         6529 g         5651 t
ORIGIN      
        1 ggtgtcggca gccattcatc gtctcaatgc ccgtgttgtc atgattgata atgttgaatt
       61 cctgttcgca ccgatcctga acctcgaccc cgtagcgatg ctgaagaagt tgagccgcga
      121 atgcccgatt atcgtcagct ggcgcggcag cctggaaggc aatactttgt attttgaaca
      181 caatggcgat ccgaaatatg ctaaattcgt catcgataac ccgaatcacg tcatttgcct
      241 cgacgaataa aaataggcgt aatttaaaga ttcgttattt tttcgcaatc gttttttcat
      301 agatacttgt tagaatgagt gctgtcccgc aaagaaaagc tctttgcgcg gcagcacttt
      361 ttttttacct gccattgtgg tatactttct agtattgttg taaacatttc atcaggtatt
      421 cagcgaaaag gagttaaaaa catgagtgat tatattattg ccgtcatcct gggtatcgtc
      481 gaaggggtca cggaatattt gccagtttca tcgacaggcc atatgattct cgtcggcgat
      541 atgctcggct ttaccggtcc cagggccagc gtctttgaag tcttcatcca gctcggcgct
      601 atcttgtcgg tcatcgtcat ttataaagag aagttcatcc gcatgctcca tcagtaccgc
      661 tgctgggacc tcttccgccg ggaaaactgg ttccgcaccg atacgggact gaccctggcc
      721 cacgtcgcag ccggcatcgt gccggtcatg ggcatcggct atctggccca ccacgccatc
      781 aagacctatc tcttttcggc agggacggtc atcatcggcc tcatcatcgg tggtatcttc
      841 atgctcgtcg ctgaaaagtc ccataccaag acggtctgta acgacgtcga aaagatgact
      901 attgcccagg ccttcctgac gggcatgttc cagatcctgg ccttatggcc cggcttttcc
      961 cgttccggtt cgaccattgc cggcggcctt ttcctgggcc tcagccggaa agcagcagct
     1021 gacttttcct tcatcatcgc cgtaccggtc atgatcgtcg cctgtttcta tgacctcttg
     1081 aagagcctga gcgacctcag cggcaacgac ctggtcatga tcgtcatcgg cttcattacg
     1141 gccttcatcg tcgcctacat ctcggtcctc tggttcctga aattcatgaa caagtcgacg
     1201 ctggcatcct ttgcctacta ccgtttcgtc gtagccgtca tttccttcgt ctacttcttc
     1261 atcctgtagg gcgttggcct gccatcatgc gagtatggtt tgtagtacgg agcttgtagt
     1321 tgtcagagaa aacctttaaa tttaaagcca tcccctaacc actagccact agctgctaac
     1381 cctaaaaagg ggctgtcgta cgtgcgacag nnnnnnnnnn cccctttttt tgaatcaggt
     1441 tggcgggatc cccatggcgg gcgccccacg tggtcgcccc tacggatgcc gatggcggga
     1501 ttcccacaac gggccgcaac cacgagccac taggccacag acgccatcac gatccgctgc
     1561 cggcgagctg gcggaattcg ttggccagtt cgtccaggaa ggggatgatc ttggcgtcgt
     1621 cgatgtcggc gttgaaggct tccatcatgt tggggaagaa gatgtacagg ccctgcatat
     1681 agacgtagta gtaatactgc aggtcgtcat ggctcatgat ggccaaggcc agggcgtcgt
     1741 tgacggcgaa gttgaggtcg tagaagtcga ggatttcatc tttgaagcgg cctaagtcga
     1801 gctcgccgcg ctggtagtct tccatgaggt ggctgcccat ttctcccaag gcatcggcaa
     1861 tgtcgccgac gttgtccgga acggcaaggc cggcgtcttc catgatgtgc gtcaaggtgt
     1921 ggagcgacgt gatcagggat tcacagaaaa aattaaaggt cagggctcct tcagagccgc
     1981 gcagattcgt aaagacagta gattccataa ggacctcctt gaagtaattt tttctattat
     2041 agcatatttt gccggaaatg aattgaatcg aggccatgaa tatagtagga taatactaag
     2101 agtattttat aggaggtaat cgttgtgtct caacgcaatc aaaaaattaa attgaagaag
     2161 cggtcccgcc aatcggcacg tatccatcgc ggctttgcca tgcctgtcgg tccgggcagt
     2221 gaaaacagcc tggtcagttt ccccgacttt gccgatctga aggccaagta tttccgcttt
     2281 accggccgca tccagcgccg gccttttatt ttccggacgc tgatcttgat gttcgcccag
     2341 ttcatgtttt ccatcatcct ctacagcaag atcgtcgaat ctattttaat caatcatatg
     2401 gaatatgcca tcatcttcgg tatcatcttc gttctgctgt ccattccggc tatttggtcc
     2461 cagatttccc tgggcgtccg gcgctgtcac gacatcaata agcccggcct catgtttgcc
     2521 gttcccttca tttgttacct ggccagctat gtcctgcccg tcctgggaca ggatacggcg
     2581 gcgacggcag cccagtcgat catggctgta tcctatttag gcttgttcac ggtcaagggg
     2641 acgtccggcg ataacgctta cggtccggaa cgggcacgat agggggcggt agcatgacct
     2701 tagcagaagg aaaaatcgga cacgactata tcatcgaaac attggatttg ccggaccaga
     2761 cggaaaagcg cctccaggcc ctgggcatga ttgaagggac ggaaatctcc gtgctcaata
     2821 ataagaacga agggacggtc atcatcaaga tgcgcagcac ccgcctggcc ttgggcaagg
     2881 gcatttcgtc gcatatcggc ataaggggga aagaccatga ctagcgaagt aaaaataaag
     2941 gctggccagc cgcaggtcca gaaggcaccg gtcaaggctg aaaaaaagga aatgaccgtc
     3001 gctttcatcg gcaatccgaa ctgcggcaag acgacgctgt tcaacgccta tacgggggcc
     3061 aacctcaagg tcgccaactg gcccggcgtt accgtcgaaa aagttgaagg cgccatcaat
     3121 gcccacggct atcacattca tctcgtcgac ctgcccggga cgtacagctt gacgtcgtac
     3181 accatggaag aacaggtttc gcggaatttc atcctcagtg acgatgtcga cgtcatcgtc
     3241 gatgtcatcg acgcgtcggc gctggaacgc aatctctacc tgaccttgca gctcctggaa
     3301 ctggggaaac ccgtcgtcgt cgccttgaac atgatggaca tcgtcgaaaa acggggcatg
     3361 gaaatcgact tgcaccgcct gccggaaatg ctcggcgtgc cggtcatccc cgtatcggcc
     3421 ctgaagcgcc gcggcctgag cgtcctgctc catgcggccg tgcaccacaa ggaccacgtc
     3481 cacccggatc ggctcatcca caaccattca gaccataccc atcatcagca taatcatcac
     3541 agtgaattcg ccatggtcta cagcgacgcc atggaagacc gcatcgacgc cgtgcgccag
     3601 ctcctgcatg acaaatatcc cgatttgtac aatcaccgct ggcatgcgct gaagctcatc
     3661 gaaagggacc cggaaattac ggccaagtat cccgtcgacg acgtccatca cgtcctggac
     3721 cgcagttatg aaacggatat catcaccgaa aaatatgcct tcatcgaaga aatcatccat
     3781 gaagtcgtcg tcaaccaggc gtcgaaagca gcggcaacgg attccgtcga ccgctacttg
     3841 accgataaat ggctgggcat ccccatcttc ctggccatca tggccatcgt ctttttcctg
     3901 actttcttca tcggcgatat ggtccaggat tccttccagg gctccattga tactttatcg
     3961 gccctggctg aaggcggcct gacttcgctg ggggctgacg atatgctcat ttccctggtc
     4021 tgtgatggca tcatcgccgg cgtcggcggc atcctgacct ttttgcccaa catctgtatc
     4081 ctcttctttg ccctggccat cctggaagac agcggttaca tgtcccgcgt ggcctatgtc
     4141 atgaacgata tcatgaaccg tttgggtttg tcgggccggg ccttcatccc catgatcctc
     4201 ggctttggtt gcagtgtgcc ggccatcatg gcgtcgcgtg ctctggagga taaacgcgac
     4261 cgctatcgga cgatgctggt tacgcctttc atgtcctgca gtgcccgcct gcccatttac
     4321 atcctctttt cgggcatgtt cttcccgcac aatgccatga ttgtcgccta ttccatgtac
     4381 ctcatcggca tcgtcgtcgc cctggcagcc ctgaaggcga tgaatctctt cagtcatgag
     4441 aaaaaggaaa acgacctgct catcgagctg ccggaatata agcggcccag tgcccatacg
     4501 gtctacgtct atgtctggga aaaaatcaag gattacctga gccgtgccgg tacagtcatc
     4561 tttgccgctt ccatcctcat gtggttcatc ctcaacttcg gccctgccgg ctatggcgat
     4621 atggccgaca gtttcggggc catcatcggc aaggccatcg tgcctttctt tgcgcccatc
     4681 ggcctggggt actggcagat ctgtctggcc ctcatcgctg gcatttcggc caaggaagtc
     4741 gtcgtatcca gtttcgccgt cctcttcgat ggcgtcaaca tcaattctcc ggcagccctg
     4801 gcggctttgg cctctatgat gagccaaggc ggctttactc agctcaatgc ctactgcatg
     4861 atgctcttct cgctgctcta tattccctgt gccgccacga tagccacgat ccacgccgaa
     4921 tcgcacagct ggaagtggac ggctttctcc gtctgtttcc agattgccgt tgcctgggtc
     4981 ctgaccttcg tcgtctatca gatcggaagt cggatttttt aatcactaat aactaaggag
     5041 tgcagtatga cagaacctat gataaaaacg gatcatctca gccataccta tgaagacgaa
     5101 gacggcaagg tggtctatgc tttgaaggat atttccctgg aaatcggcaa gggcgagttc
     5161 gtcgccatca tcgggaccaa cggttcgggc aagtcgacgc tggccaagca tttcaacgtc
     5221 ctcctgacgc cgacgtccgg cacgtgtgaa gtctgcggca tgcggaccga tgaccccgac
     5281 aatatctgga agatacgcca gcacgtcggc atggtcttcc agaatccgga taaccagatc
     5341 gtcgccgccg tcgtcaaaga agacgtggcc tttggcccgg aaaacctcgg cgtcccgtcg
     5401 gacgagatca agacccgcgt cagcgatgcc ctgcgccgcg tcaacatgac catttatgcc
     5461 aagcacggac cgcacctcct gagcggcggc cagaaacagc gcgtcgccat tgccggtatc
     5521 ctggccatgc agtgcgactg catcgtcctc gacgaaccga cggccatgct cgaccctgtc
     5581 ggccgcaagg aagtcatgga tacgctccac cagctccatg acgaaggcat taccatcatc
     5641 atcatcaccc atttcatgga agaagccgtc caggccgacc gggtcgtcgt cgtcgaccag
     5701 gccgaactga agatggacgg ggcgccgcgt gacgtcttta cgcacgtcaa agagctgaag
     5761 gccatgggcc tcgacgtacc ggtagcggcg gaactggccg aacggctgcg ccagaaaggc
     5821 ctcgacctgc cggactccat cattaccgag gaagaattag gagatgcatt atgtcaatta
     5881 agttaagcaa cgtgacctat acctacggca tcgggacgcc ttttgaaaag acggccctct
     5941 acgatacaga tgtcgaaatc catgaagggg aattcgtcgg catcatcggt cataccggat
     6001 cgggcaagtc gacgctggtc cagatcctca acggcctcat caagccgact tccggaacgg
     6061 tgaccgtcga cgggacggat atcggcaaga agacgaaaga agccatggcc gtgcgccata
     6121 aagtcggtat ggtcttccag tatccggagt atcagctctt tgaagaaact attgccaaag
     6181 acatcgcctt cgggccgcgc aatttcggtc tgagcgaaga agaaatcacg gaacgggtca
     6241 aagaagccat ggacttcgtc ggcctcgatt ataagaccta tgccgaccgt tcgccgttcc
     6301 gcctgagtgg cggccagatg cgccgcgtcg ccattgccgg cgtcatcgcc atccatccgt
     6361 cgtatctcat cctggacgaa ccgtcagccg gcctggatcc tatcggccgg cgggaaatct
     6421 tttcggaaat ccagcgctgg caccaggaaa agggcattac cgtcatcttg gtatcgcaca
     6481 acatggacga tatttcccgc ctggccagcc gcctcctggt cttgtcccac ggccatatcg
     6541 tcctcgacgg cgaaccgctg aacatctttg cgactcagca gcaggccctg cacgatgccg
     6601 gcgtgtccgt gccgcctgta acgggtgccc tccaatatct ccagggccgg ggctttgccg
     6661 tcgacgaccg ggtacggacg gtcgaagaag gggcacaggc tatttttgac tgcctgaaag
     6721 gaggcaaggg ccatgctcac tgatattact ttgggccagt attttcctgg cacgtctttt
     6781 ttgcataaac tcgatccgcg catcaagatc gtcgctacga tgatcttcat cgtcgccatc
     6841 ttttttgccc attccctggc atcctatgcc atcgtcacgg ccttcgtggc cttgaatttc
     6901 gccatttccc gcctgccggc gaagttcatc gtcaagtcgc tgaagccctt gtgggtcatc
     6961 atcattttca ccatgggcgt ccatatcttc acgacgccag gcgatgtcct ctggcagtac
     7021 ggcattttcc acatcaccaa agaaggcctg tatcaagggg ccctcatgac ggcccgcctg
     7081 gtcttcctca tcgtcttttc gtcgctgttg acgtatacga cgtcgcctat cgtcctgacc
     7141 gacggcatcg aacacctgct caatcccttt aagcgcatcg gtgtgccggc tcacgaactg
     7201 gccatgatga tgaccattgc cctgcgcttc atcccgaccc ttttggaaga aacggaccgc
     7261 atcatgaagg cccagacggc gcggggtgcc aattttacca gcggcagcct cttgcagcgc
     7321 ggcaagaaca tgatccccct gctggtaccg ctcttcgtca gcgccttccg ccgggccgat
     7381 gacctggcga cggccatgga agcccgctgc taccgcggcg gtgaagggcg gacgcggatg
     7441 aatgaattgt cctataccta tcgggacggc atcgccatgg cggctgtcct gattgtcacg
     7501 gccgtcctgg ccgctatgcg gtggttatga tgaagaagaa tatctgcctc gtcgtcgcct
     7561 atgacggtac ggatttccac ggcttccagc gccagaccaa cgccgagtcg atccagggct
     7621 gcatcgaaca gggcttgtcg gctattttcc agacgcctat tacgatttat ggcgccgccc
     7681 gcacggatgc cggtgtccac gcccgcggcc aggtcgtcaa tttctatggt caggaatcga
     7741 ttccgacgga aaagattccc tatgccatga aaggctatct gccgccggaa attgccgtcc
     7801 tgtcggcccg ggaaatgccc aatcgcttca gcgtccgcca cgataatgtg ggcaagcatt
     7861 accgctattc catcgacaac aaccgcatgc acgacccctt tctcctgcgc tatgcctggt
     7921 tcatccgcaa gcccctggac cggcaggcca tgcgggaagg ggcggccatc ctcttgggaa
     7981 cgcatgattt ttctgctttt gaaggccaga atacgacgcc catgaatcct gtgaagacga
     8041 tgtattccat taccctccat gagcggggcc atgtcctaga catcgacgtc gtcggcgacg
     8101 gcttcctcta ccatatggtg cggaacattg ccggcggcct cgtcgacctg ggcctgcacc
     8161 gcctgacacc ggagcgcttc cgggaaatcc tcgccagcgg cgaccggact cagctcgggg
     8221 cgacggcacc gccccagggc ctgtgcctgg agaccgtctt ttatgatgaa ggggagctga
     8281 aagggcgcat tgccgaatta gaaggagaat gagccttttt acagcgctga tgggcataat
     8341 atgctataat aaaagagaat agtatagttc cgtttttttc aggcggaggt gaggaccatg
     8401 ctgaccagct tactgaaaac cattactaaa ggaaaagacc ttagcgaacg ggaaagcgct
     8461 atcgtcatgg agcagatcat ggaagggacg atttcagacg tccagttggc tgcttttttg
     8521 gcagccttga cgacgaaagg cgcttcagag cgggaaatca cggcctttgc ccggaccatg
     8581 cggaaataca gtatgcatgt tccctgtacg atggacgtct tcgacatcgt cgggaccggc
     8641 ggcggccgga ccaagacctt caacatctcg acgacggcgt ctttcgtcat cgccgccggt
     8701 ggcgtccgcg tcgccaagca cggcaaccgg gccaagacgt cgcgcagtgg ttctgccgac
     8761 gttctggaag ccttaggggc caatatcttc ctcagccccg aaagctgcct ggatatgctg
     8821 gggcagatcg gcctctgtta tttctatacg cgttactact attatatgat gaaacgtatc
     8881 gatgccgtcc gcgaacagtt gggtatctat acggtcttcg atgtcctgcg gcccttgacc
     8941 aatccggctc atgcagtcta tgagatcctc ggcgtcaatg cgccgcacct ggtcgaaccc
     9001 atggcccatg tcctggctgc cctcggcgtc cgccacggca tggtcgtcta tggtcaggac
     9061 ggcagcgatg aaatctcggc atcggcagcg acgacgattt gcgaatttac gccagaatcg
     9121 ttttcgacgt atgaaatcag gccggaagat ttcaacctgc ccagggcttc gcgggacgaa
     9181 ctgcgcggcg gcctgcccaa gcacaatgct gccattaccc gcgccgtcct cgacggccgc
     9241 aagggcgggg cccggacagc cgtcctcctc aatgccggcg ccggtctcta cgtcggcggc
     9301 agtgtcctca acctctatgc cggcgtccgc aaggcagccc gcctcatcga cagcggcctg
     9361 gcccggcaga aactcgatga cttcctcatc ctgagccagc gcctgggaca ggcggaaaaa
     9421 gtgaagctca tagcagacag ggacccggaa gatatggtat aataaagggt acgaaaaaat
     9481 tcattattac gtgaggaccg catttctatg aatgataaat atttaggctg gctccttcat
     9541 atcggcgggg gcatcttagt cgtcttgatg tttttatcga ttcagctcat ggcacctgat
     9601 ttttattcga tcatgtggca tctgaccgtc agtggtgatc tggatgggtt agccgattac
     9661 attgcctctt ttggcttcta tgccatcggc atcagcgtgt tcatgattgc ctttgtcaat
     9721 gccatcggcc tgccgtccat tcccttcctg accgtcaacg gcatcatctt cggcctcatt
     9781 ccgggcatca tcatttcctg gatcggcgaa gtcatcggca ttgaaatcag cttccgactg
     9841 actcggacgc tgctccgcaa gcaggcccag aaggtcattt ccaagagcaa gatgctggaa
     9901 aaactcgatt cttacagctg tatcaagaca gtcatgatgg gccgggccat cccgtatgca
     9961 ccgaatgtcg tcgtcacggc gttcagtgcc ttgagccata tttcttaccg cgaccacttc
    10021 attgccaact tcttcggcaa gatgccggcc gtcctggtcg aagtctggct gggccatgac
    10081 ctcctgcgca tccaggaaca ctggggccgc ctgctcatat tggtagccgt cgtttctttc
    10141 atttatggca tcatctggtg gcgcaagcgt cacataaaaa aaaaccgata tcatagggtg
    10201 aagtaaaaaa aaggagctgt cgcatcgcga cnnnnnnnnn ngctcctttc aggttgttag
    10261 ttgttagcgg ataggtttaa ttttttactt ttttacgaga tttcctgttc tgacgatttt
    10321 ttatcgtcct ggacgatatc agtccataac ttatcggctg tcggctgcca gtgcttggca
    10381 taggctttgc atttagcaca gaggtgttca accgattctg gtgactgcat gtccgtcgat
    10441 ttggctcccg tccgtttgac catatcgacg agggattggg gattttccaa catcgggcag
    10501 gggcggagat gattgtggtt gaacggctgc ccttttgcgt attccttaaa taagggctgc
    10561 tggagacagg tgatgaggtc ctggtcgttg atgttggcac tagagtagtg gataaagacg
    10621 cacggttcga cgtcgccgtt aggattgata tggcagtagt tgcggccgcc agcgacacag
    10681 ccatcaatga actggccatc gttctggaag tcgatagcaa aaatgggttt gccgcccttt
    10741 acgctgcgga tttcacggat acgccgtacc atataagcac gctgttccgg tgtgggcagt
    10801 aaatcgacag aggcatcgtt gccgacgggc atatagtgga agtaccaggt aaaccaggct
    10861 cccttgctga tgaggaggtc gaggaaggta tcggatgtga cggtctggat gttcttacgg
    10921 gtatagcaga tggaagtacc gaagacgata ccgtactggc gcagtaggtc catggcgtgc
    10981 atgaccttgt cgaagacgcc ttcaccgcgg cggctgtcgt tgacttcgcg gaagccttcg
    11041 acgctgaggg agaagctgag gttgccgacc ttgacgactt cctggcagaa tgcatcgtcg
    11101 atgagggtcc cattggtaaa ggcgtggaat tcgcagtccg gatgtttccg acaaagtttc
    11161 aggatgtccg ctttgcggac cagcggttcg ccgccagtga ggaggtagaa atggcagcct
    11221 acttctttgc cttggcttac gattttatcc atgacatcgt aggacaggtt cagggtgtga
    11281 ccatattcgg cagcccagca gccgatgcag tgcaggttgc aggcgctggt cgggtcgaag
    11341 aggatggccc agggaatatt gcagtgatat ttggctttgg cttcgtcccg cgtacgcaga
    11401 ccgtagaagc ccgattcaaa gcccaggtcc atgacggctg tacggactac gttggggttg
    11461 agtgtatcca gggctcggtt gaggaattgg aaccacttgc cgccttcgtc aatgagcttg
    11521 cgggcattgg caaaagattc ttcttcaaat aagtcaccga agaaggtttc cataatcgtg
    11581 gtcagtcgtt tcaaagtctt cttacgggtt tctggatttt tttcggccat gatgcggttg
    11641 accaggaggg cgaccgattc tttttgggct ttatacatga gtttattcaa gggaatcatc
    11701 tcctgaggag tttaatgaag taatgattag cggatgatga ggacgggaag gcctgaatgg
    11761 gaggatacat agcggccgac gctgcccagg aggacgcttt ggatacggct caggccgcgg
    11821 ctgcccatga cgatgaggtc cgtttccgta tcggctgcgg tctgtaagat gatcggaccg
    11881 ggcgagccga ttttatacag gcattgcaag gggacttgag atgacaattc ttcctggcag
    11941 cgcaggaagg cgttatgacc cttggtccgc atgtgagcgg ctaattcctg taatttatgc
    12001 ggttccagcg tattggggat ggccgtatcg cgcaggctgt cgctgacgct ggcgacgtag
    12061 aggacggtca gggaagcgcc atctcctttg gccaggactt cggcttgttt cagggcctgt
    12121 aaagacgatt cagacccgtc tacgggcacc aggatatgcc ggtatgggcc tttcttttca
    12181 tcgcgggcca agaggaccgg gcagggtgcg tgggtgatga cgtaagtgct gacgctgccg
    12241 agaagaaaat tcttgagggc ctggccgccg ccgcggccca tgcagatgag gtctgccgat
    12301 tgggatttct gttctgccag gatggccgct tccggggcgc tgagttcaaa aatagactgg
    12361 actgacaggg aagcgggtat catggcggca aattttttta acagtcctgt gctgttggcc
    12421 tgggccgaag ccgtcatttc atccatagtc agttcactcg tattttccat ttgcccgaac
    12481 tggttgatga tatcggccat attggcgacg ctcagcaggg taatggcacc gccagtgatc
    12541 ctggcgatgt cgatggcgtg ctgcaaggct tgtttagaat gatcggatcc gtcgataggg
    12601 acgaggatat gctgatatga tttcataaaa atacctcctt caagggtacc tttaggatag
    12661 ccttgaagga ggctggcgtc aaagtggttt tgccgtatac gaactatagc tcttgtgtta
    12721 taattaggta tacgaatatt ttttgttaca taaaggagct tttatggagt tggaaagacg
    12781 tgaatatgaa tatatccgta aaattgctga gacagggagt ttcatagcgg cagccgaaaa
    12841 attatacatc acccagccgt cgttgagcca gttcatccgc cgcatcgagc gccatgtcgg
    12901 tgcggaactc tttgaccgtt cccatcagcc tgtgaccttg accgaagccg ggcgtatcta
    12961 tctggacatc gaagggcaga tccaggactt gtgcaaggtc cgggaacagc gtatccagga
    13021 tttggggcag accgttggcg gcaccgtgcg catcggcagt tccaattatc gcagttcgac
    13081 catcctggcg cgggctattc ccatcatcaa cgaacgctat ccgcacatcg atatctgcct
    13141 ggaagaaggg acgacgcggg aattggaaga tttcgccatc cagggaaaga cggatttttc
    13201 tatcgtcgtt aagcctacag ggcataggga cttggattat atcgaacttt tccgggaaga
    13261 attgctcttg gctgtctcgc cggactgggg cctggggcgg gaaccgctgc atgacgaggg
    13321 cctccactcc cgctatcctg tctttgatta tcacagcctc gacggccatc ccttcctggt
    13381 catgaaagaa ggccaggaac tgcgccattc cttttttgcc cttgtcgaag ccctggatat
    13441 atcgccgaca gtggccctgg aaacgacgga catggccacg gcccagaccc tggccggcat
    13501 cggcgtcggc gcggctatcg taccgggtgg cctggctctg gccaatatcc cagctgtgga
    13561 accgaagtat ttttccctgc gggcctatct ggacgactgg gatatcgtcc tggcctataa
    13621 acggggacgt tacttgtcca aggccgataa agccgtcatc cgcgtcatct gtgacgtcgc
    13681 tggttgaaaa gcgctgtcgc gtagcggctt ctgccatccc ctaaccacta gctactaacc
    13741 actaaccact aaaaaaagcg ctgtctttat aagnnnnnnn nnncattccc aaacggatta
    13801 ttttgcgaag taacgagcca ggaggccatc gaatacacgg ccatgacgag cttcgtcttt
    13861 tgccatttca tgaacagtgt catggatagc gtcgaggttg agctgtttag cgagggttgc
    13921 gatttctttc ttgcctgcgc aagcgccctg ttcagcagca gcacgcattt cgaggttggt
    13981 cttggtgtcg tccgtaacga cttcgccgag gagttcagcg aatttagcag cgtgttcagc
    14041 ttcttcgaaa gcgatgcgtt tgtaggcttc agcgacttcc ggatagccct ggcggtcagc
    14101 gacacggctc atagcgaggt acatgccgac ttcggagcat tcgcctgtga agttgaggtg
    14161 gaggccttcg aggatgcgtt cgtcgacgcc tttagcgatg cctacgcgat gttcgtcagc
    14221 gtattctttg acaccagcga cgagttccgt gaatttttct ttcggagcgc cgcactgagg
    14281 acacttttcc ggagcttccg taccttcata aacaaaaccg cagataccgc atacaaattt
    14341 tttcatcatt cattcctcct gatattataa tacattttga ataaaatgga tattaactga
    14401 atactaacaa ataactttta tttgttatgg ctacattgta acagactgat gggggaaaat
    14461 caagagtaat tttgtaaaac ttttaattga aaataattat tgattaaaaa ataaggcctt
    14521 ggaggggcga ggcggcacca ttttattgca atttatacat gagtcttata taatggaggt
    14581 agtattcaga aaaagccaag acgatacgat tagcatagaa agagactgaa ttttggaggt
    14641 tacgcctatg tggaagaaaa aagtagcatg catcctggct gtcatggcct tgtgtgggac
    14701 catggctgcc ggtgcggccg attatacgat cctggaaaaa gcggataaag tcgaaacgac
    14761 ggtctacggg acgacccaga ccggttccct gaacgaccgc atcgcggcgc tggacaagct
    14821 gctcaacggt cagtcgacgg tatccggcag tctgcaggat aaaactaatg ccctatataa
    14881 agatgtatat gggaattccg gttccgattt gtccctgttg acggcagtca acctcatgca
    14941 gtggcagtat tccggccaga ttaccgatga acccctgctg gtccgcatcg aatcgctgga
    15001 acagagcatc gacggcaaga ccatgagcgg ctccctggaa ggccgtgtcc tggccctgcg
    15061 ccgcacgctc ctgggcaata agaagtacgt ctcccagacc gtgaccattc cagccaatac
    15121 gctggtgacg atgaccaata tcgacgccct caactcgaag accattcagg aaggcgacgt
    15181 cgtccgcttt gccgtggctg atgatatctg cgtcggcgac gtcatcgcca ttccccgcgg
    15241 catggaagcg acggggaccg tcaccaaggc ccggaagtcc ggccgctttg gcaaggacgg
    15301 caaaatcgaa atcacctatg acaacgtccg cgctgccgac ggttcgcccg tagccctgac
    15361 ggtcggcgat aagacgaaag aagaatataa gcgcatggcc ggtgctgtcg gcgcgtctgc
    15421 tgccggggcc atcatcctcg gaccagtcgg cctggtcggc ggccttttcg ttcatggcaa
    15481 tgaagtcgat atcccggcag ggacgacgat gtacgccgaa accaaggaaa atgccgaagt
    15541 cgtcggtttc aaggaaaatg gcgtcatgga tgacatgaag acggccaata tggctgccag
    15601 tggcgtacag gtgccgacct ttgcgcctgt caccaatagt gatgtcgaca acagcggggc
    15661 tggcgatgcc ggtacgtaca ccgacaccga tacggcgcct cagagcagca acggcgattt
    15721 gcaggccgga ctgaaagatg gtacggttac gcctgtcgac ctgtacaaca gcaaacacaa
    15781 tgacgaccag caggccgtcg taaccattca atccaataca gcaggtgacg cgaatgaata
    15841 aaacgtggac agcggcgatg atcgcagccc tgctgcctct gaccttttcg gccagtgcag
    15901 ctgatacagc gaaacttcat acagataagg taatggccgt tcccgtgaca gaaaagaaaa
    15961 tcctgcccgt cgatttggac caggcttctg cttcgcagga cgtcgtcctg ccgcagcagc
    16021 ctgtccacat gacggctgat acattcgtcg cccgcaactc cgacggctat gtcaagagcc
    16081 ggggcaatgt cgacatcaag cagggcatgg atgaaatcca tgccaactat gtcgaaggca
    16141 acatgaagag cggcgtctac catacgaaag gacccgtagt ctacgtcaac gaaaccaatg
    16201 ccctgacggg ccagaacgtg acctataaca gccagaacgg cagcatgacc atggataagg
    16261 tcgaaggctt cgtcggcccg acgacatacg tccgcgggac cgatgccgaa atgtacgata
    16321 atatcggcta tatcaagcat ggcctgatta cgacgcctca cgccgtcgcc aagacgccgg
    16381 actattatat taccggtgat gacatccgca tctatccggg cgaaaagttc acggcagaaa
    16441 atacggccct ctggttcaag cacatccgcc tgttcacata tggccattat gaaggccgcc
    16501 tggacagtga acatacgaaa tcttctttat tcacgctgct gccgcggccg acctacaaca
    16561 gcgatgacgg catcggtgtc cggggctatg ccgatgtgcc cctcaaccag tccggcgacc
    16621 tgagcttcca tgccagctat gccctgaata gcaagaatgg cttcaagccg gcggcccagc
    16681 tccagaagaa tactaaaatc ggcactttcc gttttggcta cagcaaagaa gaaagtaccg
    16741 ataacgacga taatatctgg gctacgaagt ggccggaact ggaatacttt gcaccgcgca
    16801 tccatctcgg cgatacgggt atctatatcg attccagtgc caactggggc cgctgggctg
    16861 aagacggcgt caagacgggg acgcataagg gcttccgtac ggaaatcacg catacgccca
    16921 ttcccttgtg gcagcgggcc aatgtccgct tcttcgccgg ctatcgtaag gatttatatt
    16981 ctaccaatga tgcccagcgc cgcgatccct attcgggcgt catcctcaac cagggcatca
    17041 acgaccgcct gtggaccagc ttctggtata agaaacacaa tgtcagcggc tatacgccgt
    17101 accgcttcga taccctcgat gacccgcgcc agcatggcat gtccatcggc tacgtcctga
    17161 cgccgcggga tacggtcatc ttcacttggg ctcaggacct cgacgatgac catatttcgg
    17221 accgcaatta cacctgggtc cgcgacctcc actcgttcat tgctaccatc acgtataaac
    17281 aagtcgataa agaatggcag gtcaaaataa aggccaagga cttcgatttc taaaagggga
    17341 gaggacagga aaactttttg ctatcatgcg cagtcccttt ctcttccgcg tttgtagggg
    17401 tcgccacgtg ggcgacccgt taggatttgg aggattcttc tagcaatctg attgggaatc
    17461 gtacatatcn nnnnnnnnnc cctacaatgt aaaaaagggc ttgtcgtcat gcgacaagct
    17521 ctttttttac cctcttttta cagggcgtat ttgcattttt gcgctataat ggaataaagt
    17581 gagaaaacta gattttacat gaaaggactg tcgctttatg cctttgattt actgtgtaga
    17641 agatgatgaa aatattcgtg aactcgtcgg ttatgccctg cgcagccagg acttcgaggt
    17701 ggaaaccttt gctgacagca aggaattctg gccggctttg gagaagcgga caccggcttt
    17761 gttcctcttg gatatcatgc tgcccggtga aagcggcaat gatatccttg acaaactgcg
    17821 caagactcag cagtacaaga cactgcccgt catcatgctg acggccaaga ccagcgaata
    17881 cgatatcgtc aggggcctcg atggtggcgc cgatgactac gtctgcaagc ccttcggcat
    17941 cgtcgagctc atttcccgca tccgcgccgt cctgcgccgt tcgggccggc agagtacgga
    18001 aacgaacctc tatacgtatg gcaccgtttc cctggaccag gaaaaacata tcgtaaccgt
    18061 cgacggcaag ccctgccact tgacggtcaa ggaatttgaa ctcctgcgct acctcctggt
    18121 caatacggat atcgtcctca agcgggaaca gatcatggaa gccgtttggg gcttcacgta
    18181 tgaaggcgaa agccggacca tcgatatgca catcaagagc ctgcgccaga aactgggcga
    18241 cggcggcaag attatccata ctgtgcgcgg cgtgggctat gtcatcggag ggaacgtatg
    18301 aagcggaaag tctacctcag cctgctgacc atggggctgg cctgtatggc cgtgaccctg
    18361 ctcatttcga cgtggttctt ctggaactcc acgcagcacc agatacagca ggaactggtc
    18421 atggccatgg acgtcgtcga gacgacgatc gaggaaggga aggatacgtc cgtgtatttg
    18481 aagaaactcg gcgtccacga gacggatggc ctgcgcatta cctggatcaa taccgatggc
    18541 gatgtcctct ttgaatcgga ctatgacaaa ggggtcatgg aaaatcacct ggcccggccg
    18601 gaagtccaag aggccatcga gaacggcaag gggacggccg tccgcgattc ccagaccgta
    18661 tccaaggccc tctattatta tgctaagaaa ctgcccgacg ggacaatcct gcgggtcagt
    18721 ctggaacggg atgcttttta cgcccatttc ctcagcctcc tgccctgggc gctgctgctc
    18781 ctgggcttgt cggccctggc ctgcatcaag gtgtcgcgct tgctgacgac gagcctcctc
    18841 agcccgctcc ggcagacggc ccggttcatc cgccagatca acgacaccga cgtgaagatg
    18901 gtgcagccgc cgcgggtcga ccatgaattg cagcctttgg tcgataagat ttttttgcag
    18961 agccagacca ttaccgacaa tatgcgcagc ctcgaacagc aacgcaatat catgcgcctc
    19021 atcatggaaa atctccagga aggcgtcatc ctgacggacc aggcctgcgg cattgccggt
    19081 ctcaacctgc gggccgccca tttcctcggc gccggccaga cccaggacgt cttgcagcgc
    19141 aatctgaagg aactcctgcc cgatgtatcc tgggatacgc tgcagcacat cgacagtgcc
    19201 tgtgacgtga aactgacccg ggccgaccga ctgtacctgc tgaccatcca gcctgtctat
    19261 aaagatgatg atttctatgg catgctcttc atcatcgacg acgtgaccga acaggagcac
    19321 cgggaacagc tgcgccggga attcacgtcc aacgtatcgc acgaactgaa gacgcccctc
    19381 acgtcgatca gcggctttgc cgaagtattg tcgacgggca ttttcaagaa taaagacgat
    19441 gtcatccatt tcggcacact tatccgcaag gaagccctgc gcctcttgca gatgattgaa
    19501 gaaatcatgc acctggcccg catcgaagac ggcaagcgcc aaatccatcc cgaacggctg
    19561 cgcctcagcg gtttgattca ggatatcgta gaattcatgg aaccggtcct gatggaaaag
    19621 aaagtcactg tccactgtac catggacgat acggaagtat gggcggaccg cggcctgttc
    19681 cgggaaatga tcatgaacct cctcgacaat gccgtcaagt acaaccggcc tggcggccat
    19741 gtctacatcg atgtccgcca cgacggccac aaggccttct tcgtcatccg cgacacgggc
    19801 atcggcatcc cggaagataa acagcagcgc gtctttgaac gcttttaccg ggccgattcc
    19861 agccgttccc gcaagatcaa ggggacgggc ctgggtctgg ccatcgtcaa gcacatcgtc
    19921 gaacagcatc acggtaccat tgccttgaac agcaaagaaa acgaaggcac gaccatcacc
    19981 gtcaccctgc cgatataggg gagtgtatgg aggtatctac catcatgcaa ggttcctttt
    20041 gctttatagt ttgtagaact gagtttgtag tttgcagaag atgctttaaa tttaaaacca
    20101 ttcactacaa actgcaaact aaaaactcta aaaagagact gtcttaggac ggtctctttt
    20161 tttacaatat tttacaatac atacataaag tccttacatc tccttggtag gataaaactg
    20221 tattcagaat caggaacatc tcagattcgt ttatttaaag gagaggattt tttatgaagt
    20281 ggaaaaagtt agttcttgtc gccttagctg ctaccatggc cattggggct gccggttgcg
    20341 gaagtcagca gaaagctgat tccgggaaag acgctgccgt ttccggaagc attaccggtt
    20401 ctggttcttc ggctttgctg ccgttggtca aagatgctgc cgaaaaattc aaggccaaca
    20461 ataaggacat tacaatcacc cttaatgccg gcggttctgg gacgggtctg aaacaggttt
    20521 ccgatggttc tgtcgatatg ggtaactccg acgtaccggc tgaaacgaaa ctggacaaag
    20581 ccaaagctga aaagctggaa gaccacaaag tctgcgtcat gacagtcgct acgatcatca
    20641 ataaagatgt cggcgtcaag aacttgaccc gtcagcagtt gcaggacgta ttcacggcca
    20701 aagtcaccaa ctggaaagat gtcggcggca aagatatgcc tatcgtcctg gttacccgcc
    20761 cgaagacgtc cggtacgcgt gccctgttca aacagtacgc tatcaacggt gctgaagaag
    20821 cggacaacaa atccctggaa acggataact ccggtatcct catccagagc gtcgctcaga
    20881 atccgggtgc tatcggctat gtcgctctgc cgtatctgct caataacgac acggtcgatt
    20941 ccgtttctat cgatggcgta gctccgacac tggaaaacac ctataacagt acatacagtg
    21001 tatggggtta tgaacacatc tacaccagca aagaaccgaa agctgccgtc aaggccttca
    21061 tcgaatacat cacaggcgct gattacggca aacgcatcga agaactcggc tatggcgtaa
    21121 gctcgaagat gcagaccaaa gaagttcact aatttactgg aggagcgggt atgaatacaa
    21181 tcgaaacggt agtccaacgg gctcaccgca acgagaaagc agccaaaacg ttcatcacta
    21241 tttgcggcat cggtatggca ctggtgcccc ttctcattgg cgcttttctc ttcattaaag
    21301 gtacagatac atttttcaca tttggccaca gcgtgacgga attccttttt tccggtcagt
    21361 ggaagcctag cgatacggca gaaggtggcg gtcaggtcgg cgcagctatg ttcctggctg
    21421 gctcgctggt cacctgtctc ttgtcgctga tcatttccct gcctttcagt ctggcatcgt
    21481 ccatttacat gacggaaatc gcgacgcccc agcggcgccg cctcattcag ccggctatcg
    21541 aattgttcac gggtatcccg tcggttgtct atggcttcgt cggcatgacc gtcctcattc
    21601 cggcactgcg tcatattttc cccatgcctt ttggcttttc cgttttagct gccggcattg
    21661 tcctggctat catgattttc ccgaccatta cgaccatggc cgccgatgcc atggccgccg
    21721 tgcccaagtc gtggcgtgaa gcttcttatg gcctcggcgc gacgcgctgg gaaacgattg
    21781 cccacgtcgt acttccggcc gccaagagcg gtatcttcac cggcatcatc ctgggcctgg
    21841 cccgcgcttt gggcgaagcc ctggctgtcg ccatggtcat cggccagatg aaagtcttcc
    21901 cgacgtccct gttcctgccg gcctgcacgc tgacgacggc tatttcggcc gatatgggcg
    21961 gcgccatgga aggcggcgaa tacaacgctg ctttgtggac gatggctctg gtcctgttcc
    22021 tgctgtcatt tctgttcatc tttgtaatcc accagatcaa tgccgcttcc gaattaaaag
    22081 gaggacggag ttaatatgac aagtattgaa acgaaaaaac agaaagataa aatgatgacg
    22141 gccatcttta ccggcggtgc cgcctttgcc gtcatcctgc tcatcgcctt tgtcctctac
    22201 gtcgtcgtca gcggcgtcag cggcatgacc ccgcagctcc tgtccttcag cccgaccggt
    22261 ctggggaaca tgttcttcaa tacgatttac ctcgtcgttc tggccctcgt cgccagtacc
    22321 attacgggga tccctgccgg tatcttcctg gccgaatacg ccaagaaagg gcgcatcact
    22381 cattgggtcc gcatggccgt cgaaaccctg gcctccctgc cgtctatcgt cgtcggcttg
    22441 ttcggctacc tggtcttcat cgtcatgacc ggttctcagt ggaacctcat ggccggcgcc
    22501 ctggccgtat ccatcctgac cctgcctttg gtcacgtccg ttaccgaaga cgccctgcgc
    22561 aacttgccgc cgagctaccg caacggcagc ctgggcctgg gtgcctctca ttggcagact
    22621 atctggcacg tcctcttgcc ggcctgcttc ccgcgcatca tgaccggcct catcctggcc
    22681 gccggccgtg gcttcggtga agccgctgcc ctgatgttca cggccggtat gtcgacagac
    22741 atcgactgga cgaactggga tattacggcc acgtcgtgtc cgctcaatcc cttccgtccc
    22801 ggcgaaacac tggcccttca catctgggcc ttgcgtacgg aagcccttca tgcagacgct
    22861 gcccagcagg ctgctgcgtc gtcggccatc ctgatgatca tggtcctcgt cttcagtctg
    22921 gctgcacgct acttcagcta tcgaattgac aagaaaatgg gaggaaataa ataatggcag
    22981 aaatgactac gacgctgacc ggctttgccg cagcgacgat tgaaaagaaa cgggctgcag
    23041 aagccgtaca ggaaggcgca caggctgcta cgggtacggc ggaatatacc tttgatgtca
    23101 gccacatgga cctcttctat aatgagttcc aggccttgaa agacattaac atgaaaatca
    23161 ggaaaaatga aatcacagcc ctcatcggcc cgtcgggctg tggcaagtcg acgttcctca
    23221 agacgctgaa ccgcatgaac gactgggtcg aaggctgccg ggtcactggc gatatccgct
    23281 ttgaaggcaa ggatatcttc aaggaagtcg accccctggc cctgcgttac cgtgtcggca
    23341 tggtcttcca gcagccgacg ccgttcccga agagcatcta tgaaaatatc gcttatggtc
    23401 cgcgtatcca gggcatcaag gataagaagc agctcgatgc tatcgtcgaa aagagcctgc
    23461 gccaggctgc ctgctgggat gaaatgaagg accgcctcaa taagagcgct ctcggtcttt
    23521 ccggcggcca gcagcagcgt ctctgcattg cccgtacctt ggcagcggat ccgtcagtca
    23581 tcctcatgga cgaaccgaca tcggcattgg acccgatttc gacgcagaaa attgaagacc
    23641 tggccttgga actgaaggaa aaatatacca ttgtcatcgt cacgcacagc atgcagcagg
    23701 ctcgccgcat ttccgacaag acggcatttt tcctcttagg cgaactcatt gaatacaata
    23761 ataccctgga catgtttacc aatccgtcta atcctaagac ggaagaatac attaccggcc
    23821 gtttcggtta agaggaggaa agggacttat gctggaagtt tacgaaaaat ccgtagcaga
    23881 attgaaacac gatatgatta acctgggcat gaaggtcgaa caggccgtcg aaaatgcctg
    23941 gaaagcgctg gaagacaagg acatcgaatt ggctcagcgc atttacgatg gcgacgatgt
    24001 catcgatgat ttggtcaaga actgtatgaa gaaagacttg accatcagta tgatgcaggg
    24061 tcccgtagcg gctgactatc ggagcttgat ggcgacgttg aaaatcctct ccgacttaga
    24121 acggattgcc gatcactgcg ccgacatcag ccactacgtc atccatttgg aacagaccca
    24181 tcacaacgtg cccttgccgc agggcattaa ggaaatgtac ggcgtcatgg cctccatggt
    24241 cagcgatgtt atcgatttct acaaggaacg gggcacttcg tctcagacca gcctcatgcg
    24301 tgataaagac gatatcgtcg accagtcttt caataacctc atggaaacct tggctgccga
    24361 aatggcggct catccggaaa actccaagga ttacatcgac ctggtcctgg tcataaaata
    24421 catcgaacgc atggccgacc acgccaacaa tatcgccgaa tggctcatct accgcgatac
    24481 caacgaaatc cggatttagt aatttgtagg ggcgcccacg ttgtcaagaa taacatcgtt
    24541 tacattttgt gcaagga
//
LOCUS       DAWABZ010000014        24331 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_602_length_24331_cov_3.861303, whole genome shotgun sequence.
ACCESSION   DAWABZ010000014 DAWABZ010000000
VERSION     DAWABZ010000014.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 24331)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 24331)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..24331
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_602_length_24331_cov_3.861303"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    14525..14534
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    19629..19638
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    21917..21926
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5694 a         6754 c         6436 g         5417 t
ORIGIN      
        1 gtaaatttcc gggatgaact ggacgatagc tgcaccgata gtcaccagga taccggcgat
       61 gacgaccatc tggccgattt tccctttcgt cggccgcgac ttggcatcca tttccttata
      121 aatcttggca aagatttcca tgaggaaggt aaagacgaaa aagacgatgc caatgaaagg
      181 attgagcgca gccagggcga tggtaatcaa aatcgggatg gccaggcgca cgccggggaa
      241 aaaggtgaaa ctcttgatct tcatgacatg gagctgagag tacaccgtaa aatacgtaaa
      301 gacattaaac atcaacatag cggcaataca ttcgacaggc ttggccccca tgtacaagaa
      361 catgaggagg acgcacatgc tcatattgat acccgtaaaa cgggcgaaca ccgtccccat
      421 caggccagcc acgaagaaag gcgctatggc cgataacatt tccatgaaca aaacatctcc
      481 tgacccagat tatttcagat ggtggctgcc ccgttcagtc atggggatgc cctgtttgca
      541 gagcgggcat tcttccggtt tgtacgtcgg gacggtcaaa tgcaggagag cctggacttt
      601 atctttcggt acgccgaatt cagctttacc gccgctgcgg tcgacgagga ggccgacacc
      661 gacgatgacg ccgccggctt tcttgacgac attgacgact tcctggacgg aaccgccagt
      721 cgtgacgata tcttcgacga tgaggacgcg ttcacccggt tcgatgtgga aaccgcggcg
      781 cagtgtcata acgccctttt cacgttccgt gaagatagcc cgcgtaccga gtgccttgcc
      841 gacttcatga gccagcagga tgccgccggt cataggaccc atgacggtct ggacgttctg
      901 gtccttgaaa cgttctgcca attccttgca gagtttttcc gtatattccg gatgctggag
      961 gacgttgaac ttttcaacgt acagcgggct gtgcaggccc gacgtcaaga ggaaatggcc
     1021 ttcgaggacg gccttggtgt ctactaacaa ctgtcttact tctgcttctg tcatcatgat
     1081 tgattatacc ccctgaattt ctgctaaaat cttttgggct gcttccttag gatcggcggc
     1141 cttggtaatc ggacggccga tgactaaatg agatgccccg tcgcgcaaag cctgagacgg
     1201 cgtcgtgaac cgcttctggt cattggctac ggcaaacgtc ggacggatgc ccggcgtgac
     1261 gatgaggaaa tcgggaccat tcatttcccg gatttcttta gcttcatacg gcgaagagac
     1321 ggtgccgtcg ataccggctt ccttggccag tttcgccaga ttcttgaccg attcggcaat
     1381 gctgtacttg ccgccgattt ccttccacgc gtcttcgtcg atactggtca ggaccgttac
     1441 agccagcagg cgcggccgtt cgatattcag tttggccgct tcatcgcgga cagcttcggc
     1501 tgccgcttcc atcatagccc ggccgcctgt gccgtgcagt gtcatgaggt cggcaccgag
     1561 acgggtcagg gcgcggatgc tctgggccac cgtattggga atatcgtgga ccttcaaatc
     1621 gaggaaaaca tgtttcccct ggtccttcaa ataccgtact gcatcgggac cggcactgta
     1681 aaagagttcc atcccgactt tataaaaaga aacactatcg ccaagggttt caacaagctt
     1741 cttcatatca tccagtgaat ggacgtccaa agccgtaatg atgcgatcat ctgccatagg
     1801 ctacccctcc tgtatataaa tgtatatagg catatctaat tcattgtgtc atgttttggg
     1861 aagcctgtca aggcaaaaaa cgccttactc catccattgg ctgcggtacc agtcataggt
     1921 ccggcggatt ccttcttcca aatccatttc cggcacaaaa cctaaatccc ctttcaaggc
     1981 ctcattactg aggacggacc gcaggatatc gcccgtcctg ggtgccgtat actggacggg
     2041 gaccgtatag ccgacggctt tttcaaaact gcggatcaaa tcgttgatgc tcgtttcctg
     2101 tccggtcgac acgttcatcg tgtgacagcc tttgagtggc agcgcccgga tgatggcctg
     2161 ggcgatatcg ccggcataga cgaaatcgcg ggtctgattg ccgtcgccga agaccgtaat
     2221 cccctgacgc tgagacaaaa gcttgcagaa aatactgacg acgccgcctt cgcccttttc
     2281 gccctgccgt tcgccataga cgttggcaaa gcggaagacc gtcgcatcca taccatataa
     2341 ttcgtgatag acccgcaggt aatgttccgt cgtcatctta gtgataccat aaggggacgt
     2401 cggcacgagc cgttccgttt ctttcagggg gatatggagg ttgtcgccgt agacagcggc
     2461 actggacgaa aagagaatat gccctgtgcc atggatccgg caggcttcca gcacgtggag
     2521 gacgccttcc agattaatct ggcaatcgag gacaggctgt tccactgaag tcgggaccat
     2581 cgtctgggcc gccaagggga tgacggcatc aaagtgatgg tcggcaaaaa gggcatctac
     2641 ggccgcttca tcgcagacgt cgccttctac cagttccatc ccagccggta catgttcttt
     2701 ggatccgctg gacaaattgt caaacacgac gacatccata tcgcgccgtc cctggagctg
     2761 tgccaaaatg tgtgacccaa tgaacccagc accgccagta atcaatattt ccataatttt
     2821 aagcccccta ctattaagga tacaatactc attttgtcat caatcaaata tatactacat
     2881 atttttatat attgtatcaa taaatgaagg atgaggcaat gttcctgtgc cgcggactaa
     2941 tcgtcggatt tcttccaatt taccttcact atcggcgttc tgcgaatgga aatcgaggag
     3001 cctatcgaga tagccctgtt cccgcgccag ctgctggctc cggggataga ctaaatcaaa
     3061 atagaggcag agcgcaccga cataaaagtc catgaaggtc ttcttatcct gcgtccgcac
     3121 cagtttcccc tggcgggcct gagccatgac gacatcggaa ataggctggt tttcaaagtc
     3181 agaccagtcg acgttccaga tattcttctt ttggcgcata tacatgacat agacccggaa
     3241 aatatcgatt ttgtcggcat cgcggatgag ctggcaaaag agttgctgtc gcggcgtcaa
     3301 atcccggcga agctgataga cattatgctg gtcaatagcc atacaaatca cattatcctg
     3361 actcatatcg tccagaaaat cgcggatatg accgtcagca aacaagtaat gggccccgta
     3421 tttggcatgg tccatggacc gataatcgac gaacgtatga tactcccgca gctgctcaaa
     3481 gcggccgata tcgtggagga cgccgatgag ccaggccaaa tccacatcat cctgaaacaa
     3541 atccagactc aaagcaatct gttcacacaa cccgctcacc cgcaaagaat gagcatactt
     3601 cagccggacg ccttcttcct cctggtcaaa gccctggacg taccgcgtaa attgtgatac
     3661 cacaaaatcc cgatccatac gactcaccga accaccttcc ttatgtatcc ttaatataat
     3721 tcaattataa cgttaattat atcatatacg agcagacttg ataataacgc aggccactgg
     3781 ccctatcttc gtatggcaca aatgccccgc ttcactcccc ggatgaccat gactaaaaca
     3841 atgccaatta tacaaccgac accgtcgagc accacgtcat gccacgtcga cgtccggcca
     3901 ccggaaaaca gctgaatata ttcgtcaatc gcggccacgc tgattccaat caggagcacc
     3961 caaactttat gagaacgccg cgaaactaag acagaaaaac tgctcaacac aacgcccaat
     4021 gtcgtatatt ccgtcaaatg cgccaagcgc cggatatgcc cgtcacttaa atacgatgcc
     4081 agccgcgtcc cctgaagatg tgacaaggca aaagccagca tacgccggct ctcagcatgt
     4141 gaagccacac cattctgcat agaatgccac caaatcacac cgatgacagc agaaacaggt
     4201 agtgtcaaga ttctttcgca aattcatttc atccgccagt aaatatcgta atcagatata
     4261 tggattttcc tcgtcatttg gctgctcaat ttccatcttg aatagatgct ccatgtccat
     4321 atagcgtttt gagccccatt ccgaggcggc aacatgacga agtctggcac agacgagcat
     4381 caaagcactg ttgccgtcag gaaaggcacc aatggcccgt gtacggcggc gtatctcgcg
     4441 attcacccgt tctgttaggt tattcgtgcg gatccggctc cagtgttctg ttggaaaatc
     4501 catataggtc aaggtttcgc ccacgctgtc ctcgactttc ttggctgcgg ataaaagctt
     4561 catttcacgg agcttggcgg caatggagac cgccttttcc tgtgcagctt ccttgctctc
     4621 ctgcgcgtgg attgccttga gcattaaggt aacctgcttc atcttagtct ttggcgtcac
     4681 tgtgaagacg ttccggtaaa aatggaccgt gcagcgttgg tatttggctt caggaaagac
     4741 tacagggatg ctttccagca tgccaaggca tttatcgccg ataatgagac gcacgcctgc
     4801 aaggccacgg cctttgagcc aggtgaagaa gttcaaccag ctttccctgt cttctttcat
     4861 gccttctgct gctccgagaa tctcacggaa cccgtcttta ttgacgccaa tagcgactaa
     4921 aacggagaca ttttgaatct cgccacccca gcagcgcttg aggtaaatgc cgtcgacgta
     4981 aacatacgga tattcccccg ttaaaggacg ctgacgccat tcctcgatgt gcttgtatgc
     5041 cttctggttg agatggctga tggtgccaga ggagaccttt gtgccccaaa gcaattcggt
     5101 aatatcttcg acacggcgca cggatacgcc ggccaggtac atctcaataa gggcttcctc
     5161 gacagagcat tcacggcgtt tatagcgctc gatgatggcc gtctcaaact ggatgccctt
     5221 gagcttcggc acgcgtaatc tgacttcgcc ggaagtcgtg ccaaaatttc gctcgtaatg
     5281 accggaacgg tagcccttac ggtctcccgt tcgctcatac ttgccagcgc ggacgagttc
     5341 atccgcctcg tggtcgagca gggcgttcag tgtttcctct acactgttgc gtacaagatc
     5401 ctttaaatta ttctttataa gatcctcatt aagttgtata atattaccag acatggtcta
     5461 ttgcctcctt acgaattttg tgtggtaact taattgtact agcggcaata gactatgtct
     5521 attttttatg aaattgaatt tgcgaaatat attttacctt atcgcgtctt atgtccccct
     5581 taacaatctt atcgctaatg tggtataata ccctattacg gataacttta tctcattagc
     5641 gatttttttt gaaggaggat tttttggatg cggccttttg ttcatttgca tgtccatact
     5701 caatacagtt tgttcgacgg cttgtgcaag attcccgata tggtgaagcg cgccaagaag
     5761 ctgaatatgc cggctgtggc gattacggat catggcaata tgtacggtgt catttatctg
     5821 tataaggaag ccgtagccca aggcatcaag ccgattctgg ggtgtgaagt gtatatgacc
     5881 cggggcatcc gttttgaaaa gaagacgaaa gaacggctct gtcacttgat tttgctggcc
     5941 aaagacaata agggttacca caacctcatc aaaatcgtgt ccaaaggttt tgtcgatggg
     6001 gaaaataatt accataagcc ccgcgtcgac tacgatttgc tcgaacagta tcatgaaggc
     6061 ctcatcgcca tgagtgcctg catcgaaggc cacatccagc aggatatcct caatcacaat
     6121 gaagaagggg ccaagcggac tttggaccgg ctggtttcga tttttggcaa ggatgatttt
     6181 tacttggaag tccagaacca tggtattccg gaagaaaaag ttgtccgcac ggtattcaag
     6241 cgctggtccc aggaatatgg cctaaagctg gtggctacca acgattatca ttacatcgac
     6301 cccagcgatg cagctgccca ggaggtcaag ctctgtattt ctacgggcag tactctcgat
     6361 gacccgtcgc atttccgttt ttataataat gaattttata tgaagagcgg cgacgaaatg
     6421 gccgacctct ttgccgacat gcccgaagcc ctggatacga cgctggaaat cgccgataag
     6481 tgcaacgtcg agatttcttt cagtgagcgc catctgccgc agttccccgt acccaagggg
     6541 gaaacggatt cgtcctatct gcgcaagctc tgcgagcagg ccctgccgga aaaatatgac
     6601 cccattacgc cggaagtccg caaacggctg gactatgaat tgggtgtcat cgacactatg
     6661 ggattcccgt cgtacttcct catcgtctgg gactatgtca aatacgccag ggaccatgaa
     6721 atccccgtcg ggccgggccg cggctcggca gcgggctcgg tcgtcgcgta tttgctgggc
     6781 attaccggtc tggacccgct caagtacgac ctcctctttg aacgattcct caacccggaa
     6841 cgcgtcacta tgccggatat cgacatggac ttctgttatg aaaaccgggg ccgcgtcatc
     6901 gattacgtta cccggaaata cggtgtcgac cacgtctcgc agatcatcac tttcgggacc
     6961 ttggcggcca aagccgtcct gcgtgatgtc ggccgcgtcc tcgacatccc cttgagtgaa
     7021 gtgaaccgca tcgtcaagat ggtgcctaac gaattgggca tgaccctcga caaggccctg
     7081 aagatgagca aggaattccg cgaggaatat gaacagaatg cgacggtcca ccgcctcgtc
     7141 gatttcggta aatccctgga aggcctggtc cgccattcat cgactcacgc agcgggcgtc
     7201 gtcatctcgg cggctcctgt cgacgactac gtgccggtcc aacattccaa ggaaggcgat
     7261 ttgacgacac agtacgaaaa ggacctggtc gaagaactgg gcctgctcaa aatggacttc
     7321 ctgggcttgc ggacgctgac cgtcatcggt gatgccgtca agatgatcaa aaaggaccac
     7381 ggcatcgacc tggacatcaa tgccattccc ctggatgacc cggaaacgtg ccggctcctg
     7441 accgaaggcg atacggccgg cgtcttccag atggaatcat cgggcattac caacctggtc
     7501 aaggaactgg cgccgaggca ctttgaagac atgatcccgc tcgtcgccct ctaccgtccg
     7561 gggcctttgg gcagcggcat ggtcgatgat ttcatcaagg gcagtcacgg tgaaaagaaa
     7621 gtcacctacc tgcacccgct gctggaaccg atcctcaagg atacatatgg cgtcatcctc
     7681 taccaggaac aggtcatgca gatcgcctcg gtcatgggcg gcttttcctt gggacaagct
     7741 gatttgctgc gccgcgccat gggcaagaaa aaggaatcca tcctggtcgc ccagcggtct
     7801 aatttcctgg aagggacgcg aaagaacgcc attcccgacg acattgccaa caaggtattc
     7861 gacctcatgg tctatttcgc cggctatggt ttcaacaagt cccattcggc agcttatgcc
     7921 tacgtagcct ggcagacggc gtacctcaag gcccattatc ggccggaatt catggcagcg
     7981 actatgacca gcatgatcaa cgatgtcagt aaaatcagtt actacgtcgc agagtgccgc
     8041 cgtcacggcg tccaggtcct gtcgcctgac gtcaatgcca gcatatccat gttctccgtc
     8101 caggacgggg ccatccgctt tggcctgtcc ggcgtcaaga gcgttggcgc caacgccatc
     8161 gaagccatcg tcaaggcccg cgaagagggc ggaccattca catcgctcgg cgatttctgc
     8221 agccgcgtcg attaccatat cgtcaacaag cgggccctgg aaagcctcat caaatgcggg
     8281 gctatggatt cttttggcaa gaagcggtcc caactcatgg ccgtcatcga ccaggccatc
     8341 gccatggggt ccctcagtca gaaggactac gcgtcgggtc agatggggct cttcgacgat
     8401 gccgaagatg tcatgacgga actggaatat ccggatatcg aggaatatcc catcgaaatg
     8461 cgcctggcca tggaaaagga atacgatggt ttttatttca gtggccatcc cctcaataaa
     8521 tataaggaca ttatgaagaa attgacgccc ttgtcggtcc tctatgggga agacgggcgg
     8581 cagtacgatg gcaagatgat gcgcatcggc ggcctgatta cggccaagaa gcaggtcatg
     8641 accaagcgca atgaacaaat ggccatcatc accgtcgaag acttcaccca tgccgtatcg
     8701 gtcgtcgtct tccctaagac ctatgcccgc tatcagcagg ccgtcgccgt cgatatggcc
     8761 gtcgccatcc gcggccgggc cgacatcaac gacgacagca tccagatcct ggccgaagaa
     8821 gtgaaaccgc tggaccagga aaagggagaa cccgtgcagc ccgctgccgt atcgccggca
     8881 gcagcccgcc aggctggcga ctattggcat cccggtatgg gcgccaaatt attcatcaag
     8941 atcccggccc acttggaacg gaccgatttg gccggccgca tcggccaggt cctggaacaa
     9001 catccgggac cggtcaaggt ttacttccac ctcatgggca gccgcaagac catcttgact
     9061 aatgaaaaat attgggtaga aacgacagaa gacctgcggc aaaaactgca ggccatgctg
     9121 gaaccgcggt ccctggtcgt gcagtgaggc gtatgaaggc ttctgtcatc atgcgaggtt
     9181 cctttgcttc atagtttgta gtatggagtt tgtagtttgt agaagctggt tttaaatttt
     9241 ttaccatttg ctgcaaacta aaaactacaa acttaaaact caaaaagagg ctgtcttaat
     9301 gcgacagcct ctttttgtca tttccccgga tcagccatga ttttggccag gacgacgcgg
     9361 tatttatcgt tgtattttcg catgcagtcg ttgatgtcgc ggcggatgcg ggcgatgttt
     9421 tcgacgttat attctgccgg cgggatgtgg atgtcacaga tgacattgag ccgggcatgg
     9481 cctgaggtga tgcgcaggtg gtccgtatga taacggggat atttttcgtg gacgatctta
     9541 tccaggacgt ggcggacgtc gttgattttg tggatgtctg tgcagagcgg gtccatgtgg
     9601 atattgactt ccatatggta cttgtcctgc aattcgtctt ccaggacgtc taaggcggcg
     9661 tggacttcga cgaggtcagc cttgtccggg acttcggcgt gaatcgagcc gaacatgcgg
     9721 cccgggccgt agtcgtggat gcggatgtcg tggacgccga cgacgtgggg acaattcaaa
     9781 gcagtcgcta cgatgccccg gatgagttcc gggtccggcg ccttgcccag gagaatgtta
     9841 atgacgtctt tggcaatgcc gtagccggcg tacatgatca tcaggccgat ggccgtgcct
     9901 gcagcggcgt cgatgggcag cgtcgtgaac tgctgggcaa tggtagcgac caggacgccc
     9961 gtcgtggcga tggcatcggt aatgctgtcg gcagccgtcg catcgttgac gccggaattg
    10021 attttcttgc cgatatagcg gttatagatg cacatccaga ccttgacggc gatggagatg
    10081 acgaggacgc cgatgctcca ataggagaat tccatgtctt ctggttcaaa gaatttgcca
    10141 atcgacgttt cgcagagctt gtagccgacg acgaggatga tgatggaaat ggtcagcgat
    10201 gccaggtatt caaagcggcc gtggccgaag ggatgttcct tgtctggcgg tcggttgctc
    10261 aatttggcac tgatgatgga aatgaatgac gaacacatgt cggtcaggtt gttgaaggca
    10321 tcggacaaga taccgatgct gttgctcatc atgccgacga ggaatttgct gacaaagagg
    10381 aagaggttac aaataatgcc taagatgccg ccgaggatac tgtattgttc acggacgtgt
    10441 ttgtcatcgg tatcctcata gttcttgatg aagcggcgga taatgaattc gatcatatga
    10501 aatcacccag ctttctgatt cccttcatta tagcagagat agggcgagat acaagtaata
    10561 acgagaaaga aattgagaat aaaagaaatg caaaaaaatc ataatattat cgaaaatggg
    10621 aatagctatt gagtattttg ccgggaagaa ttgcaattac tttatttttt tatataatag
    10681 aatagtgatt gaagaataat aagctcataa agtatatgat ggggaggatg tatggatata
    10741 cgaaaagcac ttatttttga tatggatggg accatgtttg atacggaacc catttcttat
    10801 cgctgttggc gggacgtatc agcccggtat ggttttgacc tggaccgctc ggtgttcgac
    10861 cgcatgctgg gacgggacaa tctgcgcatc cgtgctattt gtgatgacgc cttcggtccg
    10921 gattatccct atgacgatat ttgtcgggaa aaggtggcct tgcagctgga atattaccgc
    10981 agccatgcta ttcccatcaa gccgggcctg ctccacgtcc tgcactacgc ccgggaagaa
    11041 gggattgcct gcgccgtcgc ttcgtcatca ccgcggacac tgattgaata tttattagat
    11101 aaaacgggtg tagctccctt cttttccgtc gtccagagcg gggaagaagc ggcccatggc
    11161 aagccggatc cggatgtgtt cctcatggcc tgcgacaaag cccgtgtttc accggaccag
    11221 gcattagtat tggaagattc agagagtggc atcctggccg cccatgctgc gggaatcccg
    11281 tctatctgga tccccgattt aattactatt cccaaagacg tacaagacct ggcctggaca
    11341 tgttgccaca gcctggaaga agttccagga ctgctggcga aaggggattc ataatatgac
    11401 acagacaaag cgaagccgct ggcttttgct catgaccgta tttttaggga tgctggcagc
    11461 tattgcaccg ctgtcgacgg atatgtatct gccagccctg ccgtcgatga tgacggcctt
    11521 tggcgtgacg ccgtctgtca tccagctgac tttgacggcc tctatgaccg gcatggcgct
    11581 ggggcagatc gccgtcggcc ctatcagtga tgataagggg cgccgcctgc ccttgatggt
    11641 cggcatggcg atctttacct tgtcgacggt gggctgtatt tggtcgccgt ccattcaggt
    11701 cttcctcatc ttccggctca tccagggctg tgccggtggt gccggtatcg tcctgtcccg
    11761 ggccattgcc cgcgacatct gcaagggcag cgccttgacg cggctctttg ccatgctcat
    11821 gctggtcaat gggattgcac cgattctggc tcccgtcatc ggcggccaga tcctgcgctt
    11881 tgccccgtgg gaaggcgtct tcctctttat tgccattgtc gggctgatcc tgaccgtatc
    11941 ggccggtttg atgcgtgaaa cactgccgca gcggcgccgc gtatccggcg gcatggcagc
    12001 cagtatgaag agctttacgg ccctgttccg tcagccttat tttatggggc attgtatcat
    12061 gcagtgcttt tcctttgccg ccttttttgc ctatatttcc ggatcatcct ttgttttcca
    12121 gaacgtctac ggcgtatcag ctcaggcttt cagcctcatc tttggcatca atggtgtcgg
    12181 cctcatgatc aacggtgccg ttacgggccg gttgacaggc cgcattgctg actggaaact
    12241 ccttcggttt tccctgtgtc tggccgctgt cggcagcgtg gccctgctgg cagccttcag
    12301 taccggcatg ccgctgccgg tcatcatggt cattctcttc ttgaccgtat cgacgctgtc
    12361 catgatgagt accagcagtt tctccatggc catgcaggac cagggacgca gcgccggcag
    12421 tgcgtcggcc ctcatcggct tcttctccat gatcagcggc gccgtcatgg ccccagtcgt
    12481 cggcatcgcc ggagaccata cggccatccc catgggcatc gtcatggtca tcggcgaaat
    12541 cggcgccctc ctggccttct atgccttgat ctatccgcga catagaaaag aacattgatc
    12601 ctttgtaggg gctcgcacgt ggcgagcccg ccggggatat gcgagatact tgtaaacttg
    12661 atttgtgtga gcgggcgtcc cacgtggacg cccctacaga aaaaggagct gtcgtattac
    12721 gacagctcct ttttttcata tattccgtgc gacgagtttt tcgagtggtt cgtctggccg
    12781 ctggaagggg atatccggtt tttctgccag gtaatccagg gcaaagccca ggcaggtggc
    12841 agcgccgtag gtcaggccgt cttcgtcgag gtcgaattcc ggcgtgtggt gattggcacc
    12901 gcatcctttt tctttgtttt cgatgcccgt aaaggtcagg acgccgggat agaggcggga
    12961 cgtgatggca aaagtttccg aagccatcca gggtgtgcag tccgtcaggg cgtcggcgcc
    13021 tatgtacttt tcgacggctt tttcggcgat ggccacgcag accgggttgt tctggacttc
    13081 aaagagcggc tccggcatgt gcaggatgtc gtaacggcag tcataggtct ggcaggcatt
    13141 ggccaggatg cgcttgaatt cttcgtagaa gcgcctgcct gcatggtcgt agctgaagaa
    13201 gcggcacgta ccggcaaagg tcaggctgtt ggggatgacg ttgaggacgt cgccactgtg
    13261 caggctgccg atggagaagg tcagacattc tttcggcggg acggcccgca tccgcagggc
    13321 ctgcatgttg ccgtagaagt cgtggaaaca gtcgataggg ctttcagcca ggtccggccg
    13381 ggagccgtgg ccgccgtggc cgtcgatgcg gatttcaaag ccgaagccgc cggccatggg
    13441 cgcgtcgtgg cagacagaga ctttgcctgc cgggatgtcc cagcggacgt gggtcgcata
    13501 gcaggcgtcg atatgccagg ggctttctgt ttccaaatag cgcaggagat attccagagg
    13561 gcccgattct tcttcgcccc gttcaaacat caagacgacg gttccgtccc actggtcttt
    13621 ccagcgctgg aggcattttg cagccatgag ctgcatggcc atatggccgt cgtgaccgca
    13681 ggcgtgcatg acgccgtgat tctgagagcg gcagacgcgc gggcacgata aattccgttc
    13741 gccttcttcg atgggcaggg catcgacgtc agcccgtaag agcagcgtct tgccagggcc
    13801 tttgccgtca atccaggcga tgaggccgcc cttgtcgacc aggcggtaag aaatgccata
    13861 ttcctccagc tttttggcaa tataggccag ggtctggtct tcctttttgg acagttccgg
    13921 atgttcgtgg aaatggcggc gcaaggcgac gagttcacct tgttctttgc gtacttcttc
    13981 gagaatatcg atcatctttg cttccccctc tgtgttattc ttcaatccct atgcgggcgg
    14041 caaggccccg gtcaaaggga tgcttgattt tcttgatttc tgagacgtaa tcggccaagt
    14101 ccaggagggc cggactgggg tcgcggccag tcaggacgac ttccgtatgg gccggtttgt
    14161 cctttaagaa atcgaggacc tgctcttccg ggacgaggcc cagggcgcag gcgccgacga
    14221 gttcgtcgca gacggccagg tcgggatggt gcatttcgca gaagtcccgg actttttgca
    14281 acaatgccgt ctgggcctca caggtggcct gtttttcggc gtcagtcatc tgaaaggtga
    14341 atttcaggat ggctttgccc cgcaggaccg tgacctgcgg caggaccttg agagactcca
    14401 gctcgcctgt cggccggccc ttgaggaact gggcgaaaag aatcttgccg ccccggccgg
    14461 cacagcgcag gaccaggccc agcgaggccg tcgtcttgcc cttgccgtcg ccgcaatata
    14521 tatgnnnnnn nnnntgtggc gggcgcccca cgggggcgcc cctacggggg ttattatacc
    14581 ataatcaaaa aatggcaggt agtggtcagg cccatccatt cgtgatataa tataacatca
    14641 gttaattttt tttagaaagg aaggccggga agatggaaga acgagcccga cagctattga
    14701 cagctttaga ggatgcaggc tttgaagcct atgtcgtcgg cggtgccgtg cgcgacctgc
    14761 ttatgggaaa agaaccgcac gactatgata tcgccacctc tgcccggcct gatgatatcc
    14821 gggccgtcgc cgccaaagag gactggcaca ccgtcgagat ccacggcgaa gcctttggca
    14881 tcgtcgtcgt cgtcattgcc ggccagacct ttgaagtcgc gactttccgc ggcgaagcct
    14941 atggcgagga cagccaccgg ccggatacgg tctggtatgc tgatacattg cgcgaagatg
    15001 tcctgcgccg tgatttcacc gtcaacgcca tggccatgga ttgccgcggc gatatttacg
    15061 actacgtcgg cggtcagaag gatttgcgca ggaaacggct ggtcaccgtc ggcaatccgg
    15121 tccgccgctt ccaggaagat gccctgcgtc tgtttcgggc ctgccgcttt gcgggacagc
    15181 tcgatttcat ggcctcgaaa gatctcgtca aagccatgcc ccaggccttt ggccgcgtcc
    15241 ccggcctatc cctggaacga gtcgtcaacg aaatggaccg cctcatggtc acgccggcgg
    15301 cgtaccgggg actggacatc ctcgtccgca ccggcctcgg cgcctgctcg tgccgccaga
    15361 aggtgaacgg ctgttatgaa gctgtcccca tcctgccgga attgagtcat ctgcccgaca
    15421 cgccgcagtc gaaaccgttc catctcttcg atgcctgggt acacaccctg gcgaccgtcg
    15481 ccaacacgcc gccggatctg accatccgat atgcggcgtt gtttcacgat gtcgccaaag
    15541 gcctacccgg tatccgcggc gtccataagg gccgctatac cgattacggc cacgatgcca
    15601 agggcgcaga actggccgaa gccatcctgc tgcgctggca caagaaaccg gcctttgccc
    15661 agcgcgtggc ctggctggtc aagacgcata tgaaattcca tttctttgcc aatacggccg
    15721 aaggcgacgt cgagaaatgg cttcgccatg aagccctcga tggccctttc catcggacag
    15781 aagaactagt cgaagccgtc ggccaggcta cggctgtggc ctgcggcgat attttaggct
    15841 gcggccatgc cgacgccagc acagaaggga cggaatcctt cggggcctat atggccatgc
    15901 tggctgaggc catgccagtc agtaccaagg acctgcacta cgaccgacgg attcccgatg
    15961 cgtgcggccg ccgtacaggc gactgcctgc ggatcctctt gaagcgggtc cagaaccagg
    16021 acctggaaaa tgaggctgat gtactggccg atgcagcccg gcgctggatg aagcgccatg
    16081 aaggggaagg gcatcatgaa taaaaatgaa aagaacctga cccatcgcat ttgcagccgc
    16141 atggtcattt gcatggctat tttggcggcc atcggctttg gcaccgttac ccgcttgttt
    16201 atagagcaag tcatccatca tgacaaaaac gtggcctctg cccaggaaca gagtcagatt
    16261 gaacggaacc tgcaatcgcc gcggggcatg atactcgacc gcaatggcaa ggtcctggcc
    16321 atcagcgaga tgtccaagtc actctatgcc gacccgacca tgctcaatga agacccggcc
    16381 atggtagccg atttactggc gccttatctc cgcatctcga aggatgaaat tgaacggcgc
    16441 ctgaaagaag atacggcctt cgtctggctc gaccgccaga tggaccacga aaaatatgaa
    16501 gccgtcgaat cgctcatcaa ggaagacaag ctgcacggcc tggctttccg cgatgaaaac
    16561 cgccgtttct atccgaacgg ttcgatggct gcccaggtca tcggttttgt cggtgaaaat
    16621 gaccacggcc tggaaggcct ggaaatgatg ctggacgatg aaatccgcgg cagcaaacag
    16681 actttccgcc tgcagaccga taaccataac atccctgtct tttcgtcagc cctggaacgg
    16741 atcctgccca acaaggaacg gtccgtctgc ctgactttgg acagtaccat ccagtacgtc
    16801 gccgaaaagg gcctggacgg catcatggcc cgcagtcatc ctcaaggtgc atccattatc
    16861 gtcatgaatc ccaagaccgg tgagatattg gctatggcca gccggcctac gtatgatccc
    16921 aatgattaca gtaagggaaa taaagaagct tacaagaacc gggctgtcgt caacgtctac
    16981 gaaccggggt cgaccttcaa gcccctcatg gccgctgccg ccctggattc ggggaaatgg
    17041 cacatcggcg atgtctatca cgacacgggt tctgtccacg tcgccgaccg gacgatttac
    17101 aactgggacc acaagggcat gggcgacgtg accttgcggg aaatcatcat gtattccatc
    17161 aataccggca tggcccatgt tgccgtgacg acaggcggca agacgctgac cgattacgcc
    17221 cgccgctttg gcttcgggac gattacgggc atcgaattgt ccggtgaaca agagggcatc
    17281 ctgttcgatc ccgataagat gtccatcgtc gatacagcca cgatgggcat tggtcagagc
    17341 gtcgccgtca cgccccttca gatggtacag gccttcagtg ccatcgccaa tgaaggccgc
    17401 atgatgaaac ctttcctggt caaggaaatc gataatcctg acggttccat ttataaaaag
    17461 acagaaccga cagaagtaag gcagcccatc aatgccggta cggctgaaac gattagcaag
    17521 ttcatgggcg aagaagtcgc tatcggcggc ggccagagcg ccaaaatcga aggctatcgc
    17581 tttggcggca agaccggcac ggcccagaag aagaccgaag acagcacggg ctacgccgaa
    17641 ggccagtatg tcggctcctt catcggcttt ggtcccttgg aagacccgca gttcctggtc
    17701 ctcatcgtcg tcgatgaccc gcagggcgtc tattacggcg cccaggtcgc tgcgccggtc
    17761 ttcaaggaaa tgatgaccga aatcgtccgc atgaaaggca tccgtccgac ggaagcgatg
    17821 gaccagaaac cgctgggttc cgtcgtcatg acgccggctc atgccattcc gcccgtccat
    17881 atttccagtg atggcgtcct catgccgtct ttcatcggct ggagtacgag ggaagtcaac
    17941 gactggctca atgaagcggg cttaggcttt atccccaagg ggatggggaa ggccatccag
    18001 caatcgccgc aggcgggcag ttatgcaccg cagggcagta atgtcaccgt tattttcaaa
    18061 cggaatccat agaataacag ggggaactag ttatgcaatt actcaacggc cgggaagtgg
    18121 ccgcatttca tcgcgaacgg gtggaacgcc gcctggccga tatccaggaa gaactccata
    18181 tccagccgga actggccatc atcctcgtcg gggatgatgc gccgtcggcc atgtatgcca
    18241 gatccatgca gaagacggcc cgggccgtcg gcttgaaggc agaaatctat cagaaaccgg
    18301 attccatcag tgaagtagag cttttatcgc tcatcgataa gctcaacgaa caggcttctg
    18361 tcttcggcat cctgcccatg atgcccttgc cgcgccactt gaacagccag cgcatcatcg
    18421 actgcatcga cccgaagaaa gacgtcgatg gcctgaccga tgtcaacatc gcccatttat
    18481 atacgggacg accggggttc gtgccctgta caccgcgggc agtcattgcc atcctcgatt
    18541 attaccatat ttctctgagt ggcaaggatg tagccatcat cggccgcagc aacgtcgtcg
    18601 ggaaaccgct ggctcagctc tgcctgaacc gcaacgccac ggtcacgcac tgtcatacgc
    18661 gcacgaaaga cctgaaagcc gtctgccgcc gggcagacct ggtcatcgcc gctgccggga
    18721 gagccggctt agtgacaggc gatatgataa aacctggtgc tgtagtcatc gacgtcggca
    18781 tcaaccgcgt cgatggcaag actgtcggcg acgtcgcttt tgatgaagcg gccgccgtgg
    18841 ccggggccgt gacacccgtg ccgggcggtg ttggcgccgt gacgacgatg atggtactgg
    18901 aaaacgtcgt ctgcggcatc gcccggacag aaactatttt ataaaagtga gggagtaatc
    18961 tatgcatatc ttagataatt tcatgattca ttatttacgt cgcttcgata aacactgctt
    19021 tacggtacaa cttcacgaca agacctacat cattggcgaa ggccagccgt tgttcaacgt
    19081 tattattcac aaagacattc cgaaaaaaga attgctggcg tcgacgtcgc tggcactggg
    19141 cgaagcgtac atgcgcaaag acgtcgaaat ccaaggcgat ttgttcacgg ctcttcgctg
    19201 cttcttatcc cagacgagcc agttctcgct ggataaatcc ttgttcaaac gcatcctcta
    19261 tccgtcggaa tcgaaatcgg ctcagaagaa acaggtttcg tcccactacg atctggggaa
    19321 tgacttctat gccaaatggc tcgatccgtc catgagctat tcctgcgcat acttcaagaa
    19381 tgaagacgac agcctcgaac aggcacagca taataaagtc cattacatcc tggaaaaact
    19441 ctacctcaaa gaaggcatga ccctcctcga tatcggctgc ggctggggct atctgcttat
    19501 cgaagctgcc aagaaatatg gcgtcaaagg ctatggctgc acactcagta aagaacagtg
    19561 gaaaaaaggc caggaacgca ttgaaaaact gggcctccag gacctggtac agatcgacct
    19621 catcgactnn nnnnnnnngc cggtagacca gtacatggcg gaatcgggct tgtcgccgaa
    19681 ggccgtcagt acgaccgcat cgtcagcgtc ggcatgctgg aacacgtcgg ccgctccaat
    19741 tatccgctgt acatggaaac agccagccac ctcttgaagg acggcggcat gttcctcctc
    19801 cattacatca gcggccacga cgaaagcatc ggcaacccgt ggatgcgcaa atacatcttc
    19861 ccgggcggaa ccttgccgtc gctccgcgaa atcgtcagcc tcgcttacga cgatgacttc
    19921 caggtcatcg acgtcgaaag cctgcgccgc cattactata agaccctcat gtgctggttc
    19981 aataacttcc agaacgtccg cgatgaagtc atcgctgccc gcggcgaaga attcgcccgc
    20041 atgtgggacc tctacctctg cggctgcgcc gtatccttct acatcggcaa catcgacatc
    20101 caccagatcc tcatgaccaa aggcaccaag aacgacctgc ccatgacccg ttggtactaa
    20161 tgtagtaggg ggcgcccaaa gggcgtcccg caggccgcag gtcgcagggg cgtcccgctg
    20221 gctcccctga taggggagct ggccgccgaa ggcggtctga gagtttgatt ttctattcac
    20281 tacaaactta aaattcaaaa agagactgtc gcacatgcgg cagtctcctt ttttacgaac
    20341 cacgagccac gaaccacgaa ccactttcac tctcctatat acgcgtaaaa cgtataacca
    20401 tagctgcttt gatgataaaa tagcataaat agtatgcttg acttttatat ctacaacggc
    20461 tatactaaaa gtacagaaag cgttaaacgt tatacgaatc cggatgaatt atctttgaat
    20521 agtagaaaag gggagtgtct cttatgatta aacagatcgt gaaaacctta gacggcaacg
    20581 aagcagctgc tgacgttgcg tataacttta ctgaagtcgc tacgatttac ccgattactc
    20641 cgtcgtcgcc gatggcagaa cacgtcgacg tctggtcggc caatggccgc aagaatctct
    20701 ttggccagcc cgtcaaatta gttgaaatgc agtccgaagc cggcgctatc ggtgccgtcc
    20761 acggcgcttc ggaaacgggc agcctctgtt cgtcctttac ggcgtcgcag ggcctgatgc
    20821 tgatgattcc agtcatgcat cgcctgtcgg gcgaattgaa accggtcgtc ctgcacattg
    20881 ccgcccgtac cgtcgggacc cacaccatgt ccatcttcgg cgaccattcc gacgtcatgg
    20941 gctgtcgcaa cacgggctgg gctatgctct gcagtgccag cgtccaggaa tgtgccgacc
    21001 tggcagccgt tgcccatctg tcgaccatca aaggccgcgt tccgttcatg cacttcttcg
    21061 acggcttccg cacatcccac gaaatccaga aaatcaagat gctgccggct gaagaactgg
    21121 gcaaactcat cgaccgcgat gctctctatc atttcaaaca caccggcctg aacccggaac
    21181 atccggttat gcgcagtacc gttaccaacc cggatatgtt tttccagtcc cgcgaagcca
    21241 ataacccctg gtatcagcgc ctgccctaca ttgtccagga ctacatggac cagatcagcg
    21301 aactgacggg ccgtccgtac cacctcttcg attactacgg cgctcctgac gcagaacacg
    21361 tcgtcatcgc catgggttct gtcaccggtg ccatccagaa aactatcgac aagctctgca
    21421 aagaaggcaa gaaagtcggc ttcatccagg tccacctcta tcgtccgttc tcgctggaac
    21481 actttatgaa agccatgcct gacacggtcc gcacgattac cgtcatggac cgcacgaaag
    21541 aaccgggcgc cctgggcgaa ccgctttacg aagatgtctg cgcagccatg tcccacgtca
    21601 aacaggacgt caccgtcctg ggctgccgct atggcctgtc gtcgaaagac gtaccgccgg
    21661 catcgatcaa cgccgtcttc accaacatgg attcgctcca gccgaagaac cacttcacac
    21721 tgggcatcat cgacgatgtt acccatcatt ccctggccga cgtacctatc acggtcgaca
    21781 cggaagggac catcagctgc aagttctggg gctttggctc ggacggcacg gttggtgcca
    21841 acaagaactc catcaagatc atcggcgaca acacggatat gtatgtccag gcttactttg
    21901 aatacgatac caagaannnn nnnnnnaaga aatccggcgg cgtcaccaag tcgcccctgc
    21961 gctttggcaa gaatccgatc cgggctacgt actatatcaa atcggcggac ttcctggcct
    22021 gccataaaca ggcgtacatg gggacttacg atattgtcag tgaaatcaag gacggcggca
    22081 tcttcctgct caactgcagc tgggataaag acgacgtcgc caaccacctc agcaataaag
    22141 taaaacgcca gctcgcttcc aagcacgtcc gcttctatat catcaatgcc accaagatcg
    22201 ccgaagaaat cggcctgggc agcaaccgca ccaatacggt cctgcaggca gctttcttca
    22261 aactggccaa catcctgccc atcgacgatg ccgtgaaata catgaaagac gccattgcca
    22321 agacctatct ggccaaaggc gaacaagtca tcgccatgaa ccaggctgcc gtcgaccgcg
    22381 gcatttcgga tatcgtcgaa gtcactgtac cggaagcatg gaaagacctg ccgtctgacc
    22441 ccgtcgtcga agatacccgc gatattccgg aattcatcaa gaaagtcgtc gaaccggcta
    22501 acctgcagct cggtgatacc atcccggtca gtgaattcgt cccctatgcc gacggttcct
    22561 accccctggg cacgacgaaa tatgaaaaac gcggtatcgc atcgaccgtt cctgaatggg
    22621 acctggaaaa atgcgtccag tgtaaccgct gctccctggt ctgcccgcac gccgctatcc
    22681 gtccgtatct ggtaacggct gatgaaaaag ccaaggctcc ggctgacttc aagaccaaga
    22741 aagccatcgg caagggcctg gaagactatg aattccgcat ccaggtttcg ccgctcgact
    22801 gctacagctg cagtgcctgc gtcaatgcct gcccggctca ggccctgacc atgaagccgc
    22861 tggaaacgca gcgtcatgaa agcgtcgact gggattatgc ccagacactc cctgaaaaac
    22921 atacgactct cgataaattc tccgtcaaag gcagccagtt ccatcagccg ctcctcgaat
    22981 tcaacggcgc atgtgcaggc tgtacggaaa cggcttacat gaagatcctg acccagctct
    23041 tcgggccgcg catgattgtc gccaatgcta cgggctgtac ccaggcttgg ggctcggcaa
    23101 tgcccagcat cccgtacacg acgaactgtg aaggcttcgg gccggcctgg tcgaactcgg
    23161 tctttgaaga caacgcagaa ttcgccctcg gcatgtccct gtccatggaa cagcagcgcg
    23221 atgctatccg cattaaatcg gaagaactcc tgggcctgac tgacggtgcc ctccacgacg
    23281 ccatcaaatc ctggatcgat tccatcggcg tcgctaacga aggcgaagtc acagaaggca
    23341 tcagccggac atacatcaaa gaattgatgg ctgctcatct caccggccgg gctgcagact
    23401 tgcagaaaga aatcctggct cataaagaac acatcatcaa gaagaccatc tggatgtatg
    23461 gcggcgacgg ctgggcttat gatatcggct acggcggtct ggaccacgtt ctggctatga
    23521 acgccaacgt caatatcatg ctcgtcgaca cggaagtcta ctccaacact ggcggccagt
    23581 cgtcgaaagc tacgccaatc ggtgctgtcg ctcagttcgc tgcatcgggc cgcaagttca
    23641 acaagaaaga cttgggccgc ctgctcatga cctatggcca tatctatatc gcccaggtcg
    23701 ccctcggcgc tgacccgaac cagctcatca aagccatcaa agaagcagaa gcctacaatg
    23761 gcccgtccat catcatcgcc tatgcaccgt gcatcaacca tggcatcaaa ggcggcatgg
    23821 gcaacgccca gcacgaaatg aaacgcgccg tagactgcgg ttactggcac ctctatcgct
    23881 acaacccgct gctcaaagaa gaaggcaaga accccttcat cctcgactcc aaagaaccgc
    23941 agcaatcctt ccgcgaatac ctcatgggtg aaacgagata tgctgccctg acgagaacct
    24001 tcccggatat cgccgaagaa ctctttgcca aggccgaaga attcgctaag aagaaatacg
    24061 aaacttataa ggaattggct gataaatagg taaaacacgc taatgccata tacacacccg
    24121 aaaacagggc tgctccaaaa ggcggccctg ttttcgcaca ctcggactca gatgcggcag
    24181 agccgcgaca gactcaaaag aaggacatcg cctcggaaac caagcctccc ctcatagggg
    24241 aggtggccgt caggccgaag aggctgaagt tcaccaatgt ctatcgatga tcaaaaagca
    24301 acctctcagt cagcttgcgc tgacagctct c
//
LOCUS       DAWABZ010000015        24223 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_608_length_24223_cov_3.295349, whole genome shotgun sequence.
ACCESSION   DAWABZ010000015 DAWABZ010000000
VERSION     DAWABZ010000015.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 24223)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 24223)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..24223
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_608_length_24223_cov_3.295349"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2224..2233
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5694 a         7061 c         6680 g         4778 t
ORIGIN      
        1 ccctgctttc tgtagaaaaa gatcggaaaa tcgctgaaat cacgttgaaa gtcgacggtg
       61 tagttctccg cggcgtcgaa gccaacgatg atatgtatgc tgctatcgat ttagtctatg
      121 ataaactggt ccgccagatt cataaataca aaacaaaaat cgcccgccgc atgaagaaag
      181 gcgctttcaa tgacgctctc gtcgaagggc cggccgttcc ggaagaagcc tttgaagtcg
      241 cccgcgtcaa gaaattcgcc gctcgtccga tggacgtcga agaagctatc ttgcagatga
      301 acctcgtagg ccacgacttc ttcgtcttcc tcaactccaa gaccgacaca atcaaccgga
      361 atactagggg ttgtccgtgg ctcccttgat aggggagctg tcagcatagc tgactgagag
      421 gttgaaaaga tgaataaaaa agctgtcgca ttaagcagga atgcataatg cgacagcttt
      481 tttttgtatt aagtttgcag gagaggcaaa taatggcggt ctgcgactgc ggccggcggg
      541 ctcgccacgt gcgagcccct actcttcctc attgtggagg acgctgattt tgggagtatg
      601 gtggatggct tgttcaaatt cgtcctggat ttcctggctc ggtccgccgt cgagtttgct
      661 gacgatgtag atggcgatga gcgagaagag gaagcccggt acgatttcat agaggccgaa
      721 ccaggcgaac tgtttccaga tgaggacggt caggccgccg acgatgatgc cggccagggc
      781 gccgttcttg gtcatgttct tccagtacag ggacaggagg atggtcgggc cgaaggcggc
      841 accgaagcca gcccaggcat aggagaccat ggtcaggatg taactgttcg ggttgagggc
      901 caggacgatg gccagggcgg cgacgacgag gaccgtcagg cggctgacgt agattaattc
      961 tttatcgctg gcattcttgc ggacgaaggt atgataaaag tccttggaaa tggccgaggc
     1021 cgtgaccagc agctgcgacg aggccgtact catgatggct gccaggacgg ccgataagat
     1081 gaggcccgaa gcaaaaggcg ggaagagctg acggctcatg accaggaaga ctttttccga
     1141 atcggcgccg actaaaggcg tcgggacgat gactttgcct accaggccga ccaggacggc
     1201 agcggccagg gagatgacga cccaggtcat ggcgatgtgt ttcgatttct tcagttccgt
     1261 cgggtcgccg atggccatga agcggaccag gatatggggc tggccgaagt agccgatgcc
     1321 ccaggccagg gcggagatga tgccgatgat acccgtcgtc gtcgtgtccg gtgccatttc
     1381 caggaggttc ggtgccgtcg tagccaatcg ggtcatgacg gcgtcgcagc cgccaaaggc
     1441 gatcatgccg accaagggga ccaggacgat ggccaggaac atcatgcagc cctggatgaa
     1501 gtcggtccag cagacggcca tgaagccgcc caggaaggtg tagatgacga cgacgccggc
     1561 actgagcagt agggccgaga agtagtccat gccgaaaacc gattcaaaga gcttgccgcc
     1621 ggagacgaag cccgatgacg tgtatataag gaaaaatata aggatgaaaa tagccgagat
     1681 gatgcggatg atattttttt catcgtggaa gcggttcttc aaatagtccg gcatagtcag
     1741 gctgttgttg gccacttcgg tatagttacg caatctctgg gatacgaact gccagttgag
     1801 gtaggtaccg atggcgatgg caatccaggc agccgagata cctgtagaat aagcgaagcc
     1861 cggtacgccc atgagcatcc agccgctcat gtccgatgct tcggcactca tagaagtgat
     1921 ccatggaccc aggctgcgcc cgccgagaat gtaatcgctc agccgctgct gggaccggga
     1981 ataatagacg ccaatcgcca gcattgccag taagtagagg gcaaaggctg tcaatatgga
     2041 cacatcatat tgcatcataa tgataaaacc cctttcacgt ggtggataaa taaataataa
     2101 aatcatttta gcatattcat gcggtgttgt atatgggaaa atacctgttc tttcccaaag
     2161 gaatcccctt atattttctt aaatcccttt gaaacggttg caaaataggc gccaatcacg
     2221 gtannnnnnn nnncacggta taatgtataa ttgatataat atcacagagt agattgcaaa
     2281 ttgtgtaaat tggaggtctc ggataggtgt ttaataaact gatacaaaga ctgctcggga
     2341 ataacacgga gcatgaatta aagaagatgt ggcccattgt ccgccgcatc aatgatctgg
     2401 aaccgaagtt ggcaggcctg agcgatgcgt cgctgcagga gaagaccttc gagttcaaga
     2461 atcgtctggc caacggtgaa acgttggccg acattctgcc cgaagccttt gccgtcgtcc
     2521 gcgaagcttc gcgccgtgtg acagggatgc gccattttga cgtccagctc ctggggggta
     2581 tcgtcctcca tcgcggggat atcgccgaaa tgcgtacggg tgaaggcaag accttggtcg
     2641 cgacgctgcc ggtttacctc aatgccctga cggggaaggg cgtccacgtc gtcaccgtca
     2701 atgactacct ggctactcgt gacagtgaag acatgggccg catctataag ttcctcggtc
     2761 tgtccgtcgg gctcatcgtc cacgacctca cgtatgacca gcgccgccgg gcatataacg
     2821 ccgacgtcac ctatgggacg aacaacgaat tcgggttcga ctacctgcgc gataacatgg
     2881 tcatcagcga agaccagatg gtacagcggc cgctcaatta ttgtatcgtc gacgaagtgg
     2941 actcgatcct catcgacgaa gcgcggacgc cgctcatcat ttccggcccg ggtgaaaaat
     3001 cgacggacct gtactacacg ctggcaggca tcgtcaagac cttcaccaaa gacgactaca
     3061 cgatggacga aaaacagaag accatcgcgc cgaccgactc gggtgttgcc aaagttgaaa
     3121 agatgttggg catcagcaac atgttcgaca acgaccacct cgatttgaac cacctcgtca
     3181 tccaggccct gcgggcgcgc ttcatgatgc accgcgataa ggattacgtc gtcaagaacg
     3241 gcgaaatcgt catcgtcgat gaattcacag gccgcctcat gttcggccgc cgttacagcg
     3301 atggcctgca tcagtccatc gaagccaagg aaaacgtcaa ggtccagggc gaaagcaaga
     3361 ccctggctac gattaccttc cagaactact tccgtatgta cgacaagctg gccggcatga
     3421 ccggtacggc caagacggaa gaagacgaat tcaacaagat ctataaactc gacgtctacg
     3481 tcatcccgac caacaaaccg gctatccgcc gcgatctgcc ggacgtcatc tacaagacga
     3541 agaacgccaa gtaccgcgcc gtcgtccggg aagtcaagaa gcgccacgct acgggccagc
     3601 cgatcctggt cggcacgacg tccatcaacc agtcggaaat cctcagccag ctcctggata
     3661 aggaaaatat cgtccacaac gtgctcaacg ccaaatacca cgaaaaagaa gcggaaatca
     3721 tcaagaacgc cggccagaag ggcatggtca ccattgccac caacatggct ggccgcggca
     3781 cggacatcaa actcgggcca ggcgtcgctg aactgggcgg cctgatgatc atcggtacgg
     3841 aacgccacga aagccgccgt atcgacaatc agctgcgcgg ccgtgccggc cgtcagggcg
     3901 acccgggtac gacgcagttc ttcctgtctc tggaagacga cctcatgcgt atcttcggct
     3961 cggaaaacat ttccaagttc atggataaat tgggcatgga tgaagacgaa ccgattacgg
     4021 ccaagatgat tacccggtcc attgaaaagg cccagaagaa agtcgaaagc cacaactttg
     4081 aaatccgtaa atacgtcctg gaatatgacg atgtcatgaa ccagcagcgt gaagtcctct
     4141 acagccagcg ccgtcaggtc ctcacagccg attccctgcg cgatactatc atgggcatgg
     4201 tcgacgatat catccacgaa ggcctggaca aatacgccaa tgaaaagctc tatcctgaag
     4261 aatgggattt cgccggcctc ctgagccaga tgcagcagta tttcgtgccc aagaacgcta
     4321 cgtctgtgga agaactggaa aacctgagcc gcgtcgaagt ccaggataaa ttgaaacaga
     4381 tcgccgtcga cctctatgaa gaacgcctca aggaaatcgg cgaacagacc atgaaggaac
     4441 tggaaaaggc catcatgctc cgcgtcgtcg acagcaagtg gatggaccac ctcgacgcta
     4501 tggatgccct caaggaaggc atcaacctgc gggcttacgg ccagaagaat ccgctcgtcg
     4561 aatacaaatt cgaagcctat gaaatgttcg aggaaatgat cgaatccatc aaacggaccg
     4621 tcgtcacctt cctctatcat atccaggtca cgtacagcaa gcccgtaccg caggcggaag
     4681 atcatctcca gggcgctcac gaagtccacg aagaaagcaa acccgtcagc gccgaagaca
     4741 tcaagaaaga cgaagaacgg gaacggcaat gatggcgtag gggcgtccac gtggggcgcc
     4801 cgcgcgaatt ctgtgaacat cctattatag ttatttttat ttagaaaaag caggtgatac
     4861 ttgtgttgtt agatgaaatg cgtaaaccgc tggaagacct caaacataga atacaagaaa
     4921 tcggggattc actttaactt acctcggaaa aaagaacgta tcatggattt agatatgcag
     4981 atgagtgacc cgaccttctg ggatgacccg gacaaggcca agaagatttc ccaggaagcg
     5041 acgctgctca agacggaagt cgaaggccac gaagaactcg tccgcaaagc tgatgacctc
     5101 agcgactatt tggaaatggc catggaagac ggcgacgcct cctttgccga agacattgaa
     5161 aagcagtacc acgacctgct caaggacatc gaaaagcggg aagtacggct cctcttgtcc
     5221 ggcgaatacg acaagtgcaa cgccctcatc accttccacg ccggtgccgg cggtacggaa
     5281 gctcaggact gggcacagat gctcatccgc atgtacaccc gctgggctga acgcaatggc
     5341 tacaaactga ccgtcctgga tatgcagccc ggtgatgaag cgggcatcaa gagcgctgcc
     5401 ttccgcgtcg aaggcgaata cgcctatggc tatttgaaat cggaaaaagg cgtccaccgc
     5461 ctggtccgca tttcgccttt cgatgccgca gcccggcgcc acacgtcctt tacggctgtc
     5521 gatgtcatgc cggaactgcc ggacgacgtc gatgtcgaaa tcaatatgga cgacgtccgc
     5581 gtcgattact tccgcgcctc cggtgccggc ggccagcacg tcaacaagac gagttccgct
     5641 gtccgcatga ctcacatgcc gacgggcatc gtcgtccagt gccagaatga acggtcacag
     5701 ctgcagaata aggaaatgtg cctgaagtac ttgcgggcta agctctttga attggaacag
     5761 gaaaagcagg aaaaactgaa agccgaaatc ggcggcgtcc accaggctat cgaatggggg
     5821 agccagatcc gctcgtacgt gttccacccg tacaacctgg tcaaggacca ccggaccaat
     5881 gtcgaaacgg gcaatatcca ggctgtcatg gacggcgacc tggacatctt catcgaaggg
     5941 tatttgcagc aggaaaaaga aaagaagtta gcaaagcagg agctctaaag taaatgaaaa
     6001 aatggatgat tttctttgtc atcgtcgcag ccctcttggt cggcgccaat tcctatgcgc
     6061 cccagctggc cgaagccggc ctctgtcaag ccttgtcggc gacactcaat ctgcacagcg
     6121 atgatgtccg cgtccaggcg tcgccagggg cgaagatctt cctcggcgac atcgacgcca
     6181 ttacggttca tgccaataat ttccaggctg gcgatctgac cttcgcccac ttcgactgca
     6241 atctctacgg cgtccatttc aagccgctcc tggccctggc cgatcaggaa gtccgggtca
     6301 cccgggccga gagcggtgaa atgacggcat ccatccgcag cgaagaactg gaaaagttcc
     6361 tggtcaagaa ggtccaggga ctgacggacc cgcaggtctc ctttgaagac gacgccgtcc
     6421 gcgttcaggg acgggtgaag ctgggcggcc tcgtgtcggc ccaggctgat gtccgcggtc
     6481 attttggcat gaaagggtcg aagctcatgt tcatcccgtc gtccgtgacg gtagaaggca
     6541 tgggcatgca gatcagcacg acgcagatgg ccaatgctga gatttacgac tttgccgaat
     6601 tccccttggg aatccagccg gacagcgtca ccatgcgcga cggcctcctg accatccatg
     6661 gccaggtgag caactcatag ataaggtgaa gagaatgaca aacatgaata aacgcagtat
     6721 gctggcgatg atactggccg tctttatcgg cctcgtatgt gcgctgggca tgtgcgtaca
     6781 acgcaatgcg atagaaaatg agtcgacgac ggtagaacag gctatggatt atgatgccat
     6841 cgtcgtcatg gcacgtaacg acggctacga cctcgacacg gcacttcaga tgtgccgcga
     6901 tgccggcgtt acgagcttta ccatttacga tactaccctc aataagctga cccagcgcgg
     6961 cgaactgtcg ctggtgacga aactcggtgc cgacctctat tatcctcagt tcggcattac
     7021 cgacaaatcc tatgactact acctcatcgg caagccccag ggccagacgg acctctattt
     7081 cgacgaagtc gtatccgacc tcaagacccg cctgggtgac gataaagtca agatcatgtc
     7141 ccatggcccg taccgtattg tcggtattca aggtgtcatg ccgggcttag gcgatgtcaa
     7201 cctgggtatc ctcagcgctg acgccaagac tatcagcgac cacggcttcc acgtcatcct
     7261 gcggccgacg aattacagca acccgacgaa ggaacaggtc gctcatttct tcgaccgcgt
     7321 cgaccagatc aagaatgtat cgggcatcat gttcgtcggc aaggaagtcc tgggctatac
     7381 gccggtcaac gccgaacgca agaccatgct ggactatacg gcggacaacc tgaacgacag
     7441 ggacattcct ttctatatga tcgaatcggt caaccagctc cagtataatc agcaggacgg
     7501 catgtatgac ctggccggcc tggtccatta ccgcacggcc cgcgtctatg ccatggccaa
     7561 ggaagaactg gaaaagatta cgcctgaaga agcggctatg cgcttttaca tcagcgacct
     7621 ggaacgcaat gcccgggtca atctctatcc gctctataag aagcctctcc acggcatgaa
     7681 cctgacccag accaacttgt cttacgtcaa gatggtttcg cagaaactga cggaccgggg
     7741 ctacaccctg ggccgggctt ccatcatgcc ggcctattat ccgaaccgcc tgctcctggc
     7801 cattacggca gcggcagcag cctgcggctt cgtcttcgtc ctcaacctgc tcctgccctt
     7861 gacggaccgc aagaactaca tcctcatggg cctgggcatc gtctgtgccg tcatcggtgc
     7921 tatcgtcgcc aaaggggcct tgttcctcca ggtctgggct atcggctgtg cgacagctgc
     7981 gccgacagcg gccattctcc tggccctgga ccactggaag aagatgaaga tcactaagaa
     8041 actcggctat ggccgcgtcg tccgcgatgg caccatcggc ttgttctttg ccgtcgccgt
     8101 ggccatgatc ggcggcctct atatcgccgc catgctgggc aatatccgct tcttcatgga
     8161 attcgacttc taccgcggcg tcaagctgac cttcatcctg cccctgatcc tggtggctat
     8221 cggctatctg cgccgcttcc cgctcatggg acagaccatc gcctcgccgg aagacctaaa
     8281 ggtcttcgtc aaggatttcc tcaatattcc cattaagatg gggaccatcc ttatcctggc
     8341 cgtcctggcc ctggctggcg tcatcttcgt cggccgcagc ggccacacgg caggcgtccc
     8401 cgtaccgggc gtggaagtcg ctatgcgccg cttcctggaa aatgtcctct atgcccgccc
     8461 gcgggaaaag gaattcctca tcggccatcc ggccttctac ctcatggtcg ctgcgctgta
     8521 ccgtaaatgg ccgcagctgc tgcacttctt cctggtcatc gccgctgcca tcggcgtcgg
     8581 ctctatggtc gaaaccttcg cccatatccg cacgcctttc ctcatgtcct tcatccgcgg
     8641 catcaacggc tgggtgacgg gcatggtcat cggtatcggc ctgatcatcg ccattgccat
     8701 cgtcagctat gtgacgacgt ggctgggaaa gcaggtggcc catcgtgact agagtcgtta
     8761 tttccggcta ttacggtttt gccaatgccg gcgacgaagc catgctggcg gccattgtcg
     8821 gctccctgaa agacatcatt cccgacgtat cgattacggt catcaccggc aactgccgca
     8881 tgacccggga aaaccataac gtacaggctg tacaccgcct gaacttcttt gctatcgcag
     8941 cggccattcg ccgctgtgat attcttataa gcggcggtgg cagccttttg caggacgtga
     9001 ccagcgcccg cagcctctat tattacttgt ggatcatgaa aatcgccctg ttcttcaaga
     9061 agcccgtcat gctctacgct cagggcatcg gccccgtccg cggtgccaag gcccgccggg
     9121 ctgtgcgcga tgtcttgcag cgcgtcgccg tcatcggcgt ccgcgatgcc gattccaaag
     9181 ccgaactggc cgccatgggc gtgacccggc cgcctatcca tgtcacggct gacgccgtct
     9241 tgtccatgca tcccgtcgat aagaacatcg gcctctatat tctcaagaaa cacggcatca
     9301 ccggcatccg ccggcgcctg ggcattgccg tccgcaactg gcaggaccat gaagcctata
     9361 aggacgccat tgccgctgct gttgacgagc tgcaggaaac acaggacgtc cacgtcatct
     9421 tcatccccat gcagtacccg gccgacgtcg aagccggccg cgacattgcc agccgcatga
     9481 agacgaaagc cgtcgtcctc gacggccact atacgacggt ggaattcatg tccctcatgg
     9541 gctgtatgga tgcggtcatt gccaaccgcc tgcatgccct catcttttcg gctatcatgc
     9601 aggtgccggc gacggccgtg tcgtatgacc cgaaaatcga cagtttcatc gacctcatcg
     9661 gcgatagggt ctgcggcacg acagaagacg tccaggctgc ggatatcgtc gccgacgtga
     9721 gccagaaatt ggctctcggc catttggcgc cggaaatcaa ggcccgcctc aatcacctgc
     9781 ggcgccagtc cctgcgcaat gcctatctgg ccctgcgcat cctggaaggc cgcaagggcg
     9841 tccgcaagcg cctgcaacag catcggaggt ccttatgatt gaattaaaga acgtatccaa
     9901 ggtctatgac aatggctcca ttgccctgga ccacgtatcg ctccatatcg gcaagggcga
     9961 gttcgtcttc gtcgtcggcg ccagcggtgc cggcaagtcg acgctcatca agctcttgtc
    10021 ctatgaagaa ctgccatcgc ggggttcggt cttcgtcaac ggcattgaag tcaataagct
    10081 ggccaagaac cgcgtgccct atctgcgccg taaaatgggc atcgtcttcc aggatttccg
    10141 cctcctgccc aataagacgg ctcaggaaaa cgtggccttt gccatggaag tcatcgaagc
    10201 gccgcggcgc ctcatccgcc gccgtgtccg cgatgtcctc gacctggtcg gcctggtcgg
    10261 caaggccgat gccctgccta agaacctcag cggcggcgaa cagcagcgcg tagccatcgc
    10321 cagggccatc gtcaaccggc cgctgctgct cattgccgat gaaccgacgg ggaacctgga
    10381 cccggaaacg tcgcgggaca ttatggaagt cttcaagaaa atcaaccaca tggggacgac
    10441 agtcctcatg gtgactcatg ataagaccct cgtcgacatg atgaacaagc gggtcatcga
    10501 aatcgacggc ggcaaagtcg tccgcgatga acagaagggg ggctatgacg atgaaaatta
    10561 ggaccatgcg ctactacatg gccgaagcca ttcagtcgtt ctggcgcaac cgcttcatgt
    10621 ccatcgcgtc cattgctacc gtcgccttgt ccctgttcat cctgggcatt ttcctgaccc
    10681 tcgtggccaa cctcgattat ttcgctgaga acctggaaag ccaggtcgaa atcacggtct
    10741 atctcaaaga cgacgtgacc cagaaggaca tcgacagcgt cggccgccgc ctgagcgccc
    10801 tgcccgatgt gaagtccgta tcctttaccg acaaggacca ggccatggac atcctcaagg
    10861 accgcatgaa ggaccagccg ggcatcctcg acgccctgga cggcaagaat cccctgccca
    10921 gttcttatga agtgaccttt accgaaccgg aagccgtgcg ccggacggcc gacatcgtcg
    10981 ctgactatcc cgaagtcgaa gggacccatt acggccagga aatcatcgaa cagctcttcc
    11041 agatcacacg catcatccgc tggggcggca ttgccctgat catcttcctg accctggcta
    11101 tgctgttcat catttccaac actatccgcc tgaccgtctt cgccaggcgc aaggaaatcg
    11161 ccatcatgaa atacgtcggc gcgacgaact ggttcatccg ctggccgttc ctcctggaag
    11221 ggctgctcct gggcttcatc ggcggcgtcc tggccgacct ggccttgtgc cagttctacg
    11281 gcttcgtcgt cacggcggtc caccagtccc tggccttcct gcccatggtc agcgtctatc
    11341 cctttatgta ccgcacggca gccattctct tggtcatcag tatgatcatc ggcgccctgg
    11401 gcagtacgat ttcgctgaaa cgatatatga aggtgtaata tggtaagaaa atatatcatc
    11461 ctctccgtga cggcagccct gttatcgccg tgtctcctga cctttgccga cgatgacctg
    11521 cagggccagc tggacgacgt ccagacccgc atggccgagc agagtgagaa gaaggcccag
    11581 gcccaaatgg tcatcgacaa cgtcagcgat aagctctatg aaatccagaa gagcctggaa
    11641 gccgctcagg gcgaatacca gacggtgacg gccgatttgg cggcgacgga agaaaaaata
    11701 gccgcgaccc aggccgagct cgacgagaac cgggcgcgcc tgcagaagcg ggagaaagtc
    11761 tttaccaagc gcatccgcga tatctatatg cacggccagc tgagttatct cgacgtcgtc
    11821 ctcggcgcca aggatttcag cgacttttcc aaccgcctgg aactcctgcg ccgcgtcgtc
    11881 gatgccgata tctccctcat ttctgacatc cgtcaggaac gggcagctat cgaagcggct
    11941 caaaaagaat tagaagtaca gcgcgaccgt caggcacagc tgcgcgatga agcgaaggcc
    12001 aagcgcgacg aaatcgccgc ccataaagac gaacagcagg ccgtccttta ccaggcccag
    12061 aacgataagg ctacggctga gaaggcctat gccgaatatc agcaggcttc ccagtccctg
    12121 ggcgacaagc tgcgccagcg ggctgccgaa gaagcggcgg cccaggccgc gccgcctgcc
    12181 ccggacagca gcgatggcag cgattcccag cccgtcagca gcggcgggac gggagccatg
    12241 atctggcccg tcaacggcgt catcacgtcg ccttacggtt accggactca tcccatcttt
    12301 gggacgacca tctaccacag cggcatcgat atcggcgtcg attatggcac gcccgtccag
    12361 gcagccgatg gcggtaccgt cgtcgaagcc ggctggatca gcggctatgg ctatgccgtc
    12421 gtcatcgacc acggcaacgg cctgtcgacg ttgtacggcc acaatcagga actcgccgtt
    12481 tcgccgggcc aggccgtctc tcaaggccag gtcatcgcct atgccggctc gacgggcaac
    12541 tccacgggcc cgcacgtcca ctttgaagtc cgcgccaatg gcgaccccgt cgacccccag
    12601 gggtatttgt aggggaaagt cggcgcggta cggcttctgc tgccaggcac gagtacctaa
    12661 tcgtttttcg ttcgtcgttc gtcgttcatc gtgacgggta attttttatc ttttacgatc
    12721 aacgatgaac ggactacgat gaactttttt tacacacaag gaggcaccct gatggggaaa
    12781 catagtttac ggcagacgtg ggagagcgtc cgccggccgc tgggatatgt ctgcagcggt
    12841 atgatcatca tgattgtcgc catggttggc ctcttttggt attatacggg ccggccttcg
    12901 tcgatgttcg agtttttccg gaccttggaa atcatcgaaa gccattatgc cgaaaatgtc
    12961 gataaaaacg ccatctttga cggtgccttg aagggcatgg tcagtacttt gggcgataaa
    13021 cattcgacct atctcggcgg cgatttgtat aaagactttt ccgcccagat gagcggcact
    13081 tacgccggca tcggcgtcta catcgcctct accgatgaag gcatcctcat cgccggtgtc
    13141 atggaaggca gtccggctga ggaagcgggt ctccagcgcg gtgatatcct ggtgtccatt
    13201 gatggcacgt ccgtcgaggg ctatcagctg gaagatgtgt ccaagaacat ccgcggacct
    13261 gtcgacacca gcgtcgacct cgtcgtccgc cgcgatggcg aagaacaatc ctttaccgtc
    13321 cagcgccgcc agatccacgt gccgacagtc gccggcaaga tggtcgacgg cacggacgtc
    13381 ggctatatcc gcgtggccgt cttcagtgat ggcacggctg acgatttcac caaggaattc
    13441 accaagctcc gcgagcaggg catgaacaag ctcatcctcg acctgcgtga caacccgggc
    13501 ggcatcgtcg aacaggctgt cggcgtggcc agcaatttcg ttccgcccaa cagtaccatc
    13561 gtgtcttata cggaacagga tggcaaagtg gaccagtaca cggcccaggg gacggatgac
    13621 ccgattccca tcgtcgtctt ggtcaatgaa aattcggcca gcgcttcgga aatcgtcgcc
    13681 ggtgccgtcc aggacatgaa actcggaccc atcgtcggcg tcaagaccta cggcaagggg
    13741 accgtccagg gcgtcttccc cgtcgactcg gcatcagccg tcaaagtgac cgtcgccaag
    13801 tatcggacga cgaatggccg cgaaatcgac ggcgtcggta tcgaaccgga tgtcgtcgtt
    13861 cccttgacgc cgtccgaccc ggaagacagc cagttcgaga aggctttgga aattattcag
    13921 gaaaaatgac tttacagatg agcgaatatg ctttaaaatt tgatagtaac gtcaattaga
    13981 tatttttgga cagatagaca ggagacatgg tatatgtatg ctgtgtctct ttctgtatcc
    14041 gcatgtattc ctatgaggtg aaatatgttt atagacagag caagaatttt tgtacagtcc
    14101 ggcaagggcg gcgacggcat gagcagtttc cgtcacgaaa agttcgtccc caagggcggg
    14161 ccgaacggcg gtgacggcgg ccagggcggc aacgtcgtcc tcgtcgccga ccgcaacgtc
    14221 aataccctgg tcgacttccg gttccgccgc ctgttcaagg ccaagcccgg cggaaaaggt
    14281 gaaggcagca acaagtacgg ccgcaatgcc gaagatttat tgatcactgt tcccttgggg
    14341 accatcgtca aagatgaaga aacgggccag gtcatggccg acttatcccg cgacggccag
    14401 cgggccatcg tcgccaaagg cggccgcggc ggccgcggca actggcattt ccgcaccagc
    14461 gccaaccgga cgccgacctt tgccgaacgg ggcgaaccgg gggaagaacg gtggctgcgc
    14521 ctggaactga aggtcctggc tgacgtcggc ctcctgggtt accccagcgt cggcaagtcg
    14581 agtatcctgc gcaaggtttc ggcagctcag ccggaagtcg cagcttacca cttcacgacg
    14641 ctcaacccga ttttgggtgt cgtcaacctg ccggaccacc gcagtttcgt catggccgat
    14701 atccccggcc tcatcgacgg cgccagtgaa ggcgtcggcc tgggccacga tttcctgcgc
    14761 cacatcgaac ggacgaagat cctcatccac gtcgtcgacg tgtcgggcat cgaagggcgc
    14821 gaccccatcg aagactatga aaagatcaat gccgaactgg cgaaatacag cgaaaaactg
    14881 gcgcgcaaac gccaggtcgt agcggccaac aaaatcgacc tcctcggcga ttcggacaac
    14941 ctggaacggc tcatggacta catgaccgct cacggcgtcg aagtctatcc catctgcgcc
    15001 atgaccggcg aaggcatgga caagctcctg gaacgggtct ggacgatgct cgaagagtac
    15061 gtcgaagaac cggacgaaac gacggaagaa gtcgtctata aagcccagaa taagccggac
    15121 tttgaagtca agcgcgacga cgacggcgcc ttcgtcatca ccggcgcccg catcgaaaac
    15181 ctcgtcgcta tgaccaactt cgacgacgac cagtccctgc gccgcttcca gcgcatctgg
    15241 cgctacatgg aactggacaa gctcctccag gaacacggca tccaggacgg caacaccgtc
    15301 cgcatttatt ccatggaatt tgaataccac aaataacgca ggccgccatt ggctcccctg
    15361 ataggggagc catcgtaggg gctcgcacgt ggcgagcccg cctgagtcca acatatttta
    15421 cataataatg agcacaagga gatccatatg aacaataaag ataagaagca actcaagggc
    15481 atggccagcc tcatgccgtc tgtcgtccag atcggcaagg acggcctcag cgatgccgtc
    15541 atcgacagcg cccgtgctgc tattacggcg cgggaactga tcaaggccca cgtcctcaac
    15601 aacgccaatc tgccgacgaa ggaaaccatg gaagatttgg ccgacatgct cggcgccgaa
    15661 ctcatccagg tcatcggcca ctacggcgtc ttattcaaga agaaagacaa gaaatctcat
    15721 tacgaattta ttaaagacta ggacgtgagg ctttgtgaac agtacaagca gggacttcgg
    15781 ccagaagaaa cgcatcgtcg tcaaggtcgg gtccagcacc atttcccatg ctacgggaaa
    15841 attgaactat cggcgtatgg aacacctggt ccgggaactg gcggatctcc agaaccaggg
    15901 taagcagatg atcctcgtca gctccggcgc gaccaatgcc ggtatggcgc cgctccacat
    15961 ggaccaccgg ccccggtcga tacgggaaaa gcaggctctc gccgccgtcg gccagggcgt
    16021 cctcatgcat acgtatgaac ggctcttccg cgaatacggc caggtcgtcg gccaggtcct
    16081 gctgacccgc atggattccc aggaccggag caagttcatg aactctcgca acgctttgct
    16141 gacgatgctc gacatgggcg tcattcccat catcaatgaa aacgacgtcg tcgccgtcga
    16201 tgaatacaaa atcggcgaca acgacacgct gtcggctatg gtcagcggcc tcgtcgacgc
    16261 cgacctgctc atcctcttgt ccgacatcga cggcctctac acggataatc cgcggaccca
    16321 tgatgatgcc aaaatcatcc ctgtcgtcga caacatcgac aagcacatct atgaaatcgc
    16381 cggcgacgcc gggtccagta tgggcacggg cggcatgtac actaagatac aggccgcgtc
    16441 cattgccacg gcagccgatg tggacatggt catcgcctcg ggcagtgaag acggcatcat
    16501 cagccgtatc tgcgccggcg aagaagtggg gacgctgttc aaagctaaag aaacgccgct
    16561 gcactcgaag aagcgctggc tcgtatcggg cagcaaggcc cagggccggc tcgtcgtcga
    16621 ccagggctgc cgcaatgcca tcatcgacca cggttcgagc ctcctgcccg tcggcattac
    16681 cgccgtcgaa ggcatcttcg atgaagggga catcgtcagc gtcgtctacg acggcctgac
    16741 cattgccaag ggcatcagca actacagcag cgtcaacatc aatgccatca agggcctgca
    16801 gtctgcggat attcccaagg tcctgggcca caatggcatt tatgaggaag tcattcatcg
    16861 cgataatctc gtcgccatgt attaaaaggg ggtattttct atgatatccg aagtattccg
    16921 caaaggccgt gacgccaagg aagcctcgta cgtcctggcc gctgcctcta cggcggaaaa
    16981 gaatcaggct ctggccgcca tcgccgacgg cctcgtcgcc catgaaaccg atatcctggc
    17041 agccaatgcc gtcgacttgc agcgggctaa agccaagggc atggcgtcgg ccatggtcga
    17101 ccgcctgacg ctgaccaaag agcgcatcgc cgccatggcc gaaggcgtcc gccaggtcat
    17161 cgccctgcca gacccggccg gcgtcgtcct cgacgaatgg gaccgcccca acggcctgca
    17221 catccgcaag gtcagcgtgc ccttaggcgt catcgccatc atttatgaat cgcggcccaa
    17281 cgtcacggtc gacgccgccg tcctctgcgt caaagccggt aatgcctgcg ttctccgcgg
    17341 cggcagtgaa gccatcgctt ccaatacggc cctggccaaa gtcatccgcg aaagcctggc
    17401 cgcagccggc cttccggccg atgctgtgaa cctcatcgag gataccgacc gggccctggt
    17461 caaagaactc ttgaccttgc gccgctatat cgacctggcc attccccgcg gcggggccgg
    17521 cctgatccgc atggtcgccg acacggcgac agtgccctgc atcgaaacgg ggagcggcgt
    17581 ctgccacgtc tacgtcgaca aggatgccga tttggacatg gccgtcaaga tcgtcgaaaa
    17641 tgccaaggtc tcgcggccgt cgacgtgcaa cgccatggaa acgctcctgg tccaccgcga
    17701 tgtggcccag gccttcctca agaaactggc gccggtcatg aaagccgacg gcgtcgagct
    17761 gcgcggtgac gacgaagcct gccagtccag cgccatgaag gctgccagtg acgacgactg
    17821 ggcgacggaa tacaacgccc tcatcatgtc ggtcaaggtc gtcggctccc tccaggaagc
    17881 actggaccat atccgccgtt tcagcactca ccattcggaa gccatcatca cggaaaacaa
    17941 ggccactgcg gctgccttcc aggccgccgt cgattcggcc gctgtctacg tcaacgcgtc
    18001 gacgcgcttt accgatggct ttgaattcgg ctttggcgcc gaaatcggca tcagtacgca
    18061 gaagctgcat gtccgcggac ccatggggct ggaagctctc gtgtcctata aatacctcgt
    18121 cgacggcgac ggtcaggtcc gttgaggctg cggaagctgc gcgggtatgg acggaccctg
    18181 cgccagtacg ctgcgtcgcc gaagacacag tttgaatacc ggcattatgc cagattttta
    18241 ttgctttacg cgttagcagt agctatcgcc tggggaggaa tctattggta ccatggaaat
    18301 cactaggctc ggcatcatgg gagggacctt caatcccatc catttaggcc atctcatgat
    18361 cgctgaagaa gcgcgccaga cctttcacct ggacaaggtc ctcttcgtgc cgtcatacat
    18421 cacgcccaac aagaacgtca acggcgcgac ggccgagcag cgcctggcca tgacgcgcct
    18481 ggctacggct ggcaacccgc attttacagt cagtgatatg gagatgcgcc gcaagggcaa
    18541 ctcctatacg gtcgatacgc tgcgcttcct gaagaagctc tacggaccgt cgtatatctt
    18601 gtattttata tccggcacag acacgatcca cgacctgtac aactggaagg aaccggaaga
    18661 aatcctgaag ctgtgccagc tcgtcggagc gacccggccc gatggctcag aacaaatcga
    18721 ttccatcatc gcctctttcg gagagctggg gaagcatatc ctcaagctgc ccgtaccgac
    18781 catggaaatc tcatcgacag aactgcgccg ccgcatccgc ttagggctat ctgtgcgcta
    18841 tatgataccg ccggctgtgg ccgaatacat cagaaaaaat ggagtatatc aatgtacaac
    18901 gaagaattca aagatagaat caaagaggat ttaaagaaat ctctgtcttc tcatcgctat
    18961 acccatgtac tgggtgtagc agcagcggcc cgcgaattgg cagaccgcta cggctgcgat
    19021 gccgatacgg ctgaagtagc cggccttttg cacgatgctg ccaaacagct gcccctgccg
    19081 gagatgcagg cactggcgga aaaatccttt cccgtcctgc cggcacctat cctggctaac
    19141 ggcggcctgc tccacggcta tgcggccgtg accatcgcca aggagcgcta tggcattacc
    19201 gacccggccc tcttgggggc cgtcgcccac cacacgaccg gggcggaaca gatgagcacc
    19261 ctggaaaaaa tcattttcct cgccgattac atcgaagtga accgcgactt cgacggtgtc
    19321 gacgacctgc gccaggcagc caggcgcgac ctcgatgaag ccgtcctctt gggctatgat
    19381 tcgacgatcc gccatctcct cgaccagggc aaacctattt tcgtcggcac cgtcgtcaac
    19441 cgcaatgccc agatcgacgt cgtccgtgcc gcagatggga aggcctgagg atgacacagg
    19501 agaaacgaag aaagatatta taccgcatcc tggccgtcgt cgccgccatc gccatcgtcc
    19561 tggcaggccg gtccctgtac ctgcattacc agtccatcaa agaggcgccg atccagcagg
    19621 atgtgccgga cacgagccag acccagagcg acagctccgt ctttgcccag accagcccgg
    19681 acaagccgct ctacgtcctg ctcctcggca ccgatggcgg ggacccgcag caggcgaatt
    19741 tcgtcggcgt cgcggctatc aacaaggcca agaagcacat cgacatcatc atgctgcctg
    19801 acgatacgaa aatcgaaggg cgcaaggaaa agggcatcca ggaattgcag gatgtctaca
    19861 gtgaaggcgg catgagcctg gtccgggccg tcgtcgaaga cattttccac atccccatcc
    19921 cgtattatgc gacctttacg gccgattcct tctcgaagat ggtcgacctc agcggcggtg
    19981 tgccgattta cgtcgaaaag aacatgtacc acggcgacga agcgacgggg aagaccgaca
    20041 tcaacctctt ccagggctat cagaccctga ccgggaagga agccgccggc tatatgcgct
    20101 atatcgacgc ggacggctat ctgtcgcgga cgcagcggca ggaacggttc atcaagaact
    20161 tctatgaaga ccggcaggac tgcttcggcg tgaccaatgc cttcttcatg taccgggcct
    20221 ggcacaacgt cgattccaac atttccgcca aggacatggc ccagctggcc ttcgatttcc
    20281 gcggtgtgac ggctgacaac atcgatttct atatcctgcc cggcgaaatg gccatgagcg
    20341 gcaagaacgg cgatacgaaa tactactgga cctacgatcc ggtcgaagtc cagaaagtca
    20401 ttggccgcac gaacaacgcc atctcgacgg cgccggaacc ggatgatacg aaacaataat
    20461 tacgtcataa aggagttact atatacatgc caattaccat gaaagcagcg aaagctgaac
    20521 aaccgaaaga actgaagagc tatgaaattg cctgcaaggc cgctgacgac aagaaagcct
    20581 gggacatcgt caccctcgat atgagcgatt cgtccctcat ggcggactac ttcgtcatct
    20641 gcagtgcccg caacaaccgg cagtcccaga gtattgccga caatatcgaa gaacagatgg
    20701 aaaagaacgg ctatgatgtc cgtcacgtcg aaggctaccg cgaaggcaag tggatcctca
    20761 tcgatgccgg tgaagtcatt gcccacgtct tcgtcgaaca ggatcgtcag tattacgatc
    20821 tggaaaactt atggtgcgac gcaccgcgca ttgcctacga aggtgaataa gatgacgaca
    20881 cttcaggaaa atagtattgt tacgctggaa gtcctgcgcc agagcgatat gggcgtcttt
    20941 ttgaacggcg gtacgggcaa tacgaacgac gatattttac tccataagaa ccagcagaca
    21001 ggcgacgtca aagtcggcga caaagtgacg gtcttcttgt accacgaccc gtcaggccgc
    21061 ctgacggcct ctatgcgcct gccgaagatc aaagtcggcc agatcggcta tgcgccggtc
    21121 atcaatacga cgcgcttcgg tgccttcgtc gacgtcggca cggaacgggg catcttcatg
    21181 ccctttgccg aaatgaaagg ccgccccaag gaaggggaat acgtctgggt caagctctat
    21241 gaagacaagt cccaccgtct ggccgtctct atggaagtgg aagatgaaat gcgccgggct
    21301 tcgcgggcgg cgacggacgc caaagtcggc gactgggtcg aaggggcggt ctacaacatg
    21361 accgaccagg gggcgtatct catgacccga gaacgctgga tcgccttcct gcaccgcagc
    21421 gaattcaacg ggcccatcct catcggccag atgatcaaag gccgcatcac ctacctgcgc
    21481 gaagacggcc gcatcaacat ctccctgcgc cagaccaagg aaaatgccat caatcccgat
    21541 gccgaacgta tcctggactt cctcaagcag cgcaagggca agatgcccta tggcgacaag
    21601 acggcaccgg ccgtcatcaa agccaaattc ggcctgagca aagccgcctt caagcgcgcc
    21661 ttgggccacc tcatgaaaga aaagaaaatc caccaggacc agggctggac ctatttggat
    21721 atataggggc tcgcgcgtgg cgagcccgca tgtggcacgt tgtgggaatc ccgcaagctg
    21781 gtgtgattga gcggttcgta taaccaacgg gaccgtgcac gaagacatat gtcgtcatgc
    21841 acggtcccgt tttgatttta taggggcttg cacgtggcaa gcccaatcca gaatatgaac
    21901 gcttttcccg tttgaggaga gtgccgccaa atacccgacg ggctccccac gtgggagacc
    21961 ctacggattg aactttttct gttagacaga aaaagttcaa tctgtcccat ttacaatggg
    22021 gtggatatac cctaaaatag agtacagatt aatcataacc atacattggc caatgtgtga
    22081 aactctataa ccgttagggg gatttcacat gtcatcatat tctatggaac acaaacaatc
    22141 ggccacttcg gctgggagcc tttgttatgc ggccgttttg acggcgttgg tcttcgtcct
    22201 cacttttgtg ccgaagattc ccattccact gggctatgct catttgggag acgcgattat
    22261 ttttgcgtcg gtcctgttca gtacgcgccg gcaggcggcc ctggctgcct ctatcgggtc
    22321 ggccctgtcc gatttcattg gcggtttccc gatttggatc atcccgacgc tgatcatcaa
    22381 gtacgtcatg gtcgaagccg tcttttatac ggtacgcccg gacaaacggg aatggacgct
    22441 gacgtcgccg cgcgtcttca tcggtttctt cttatccgct gcctggatgg tcctgtctta
    22501 taccgtatcg ggcagtctcc tctacggcag ctgggccgtc ggcatcccca tggtgccggg
    22561 actcatcggc gaaggcgtcg tcaatatgat tgccgcttat gcactggccg cttgtctggt
    22621 caaggctaaa ttccatggtt atggttatag cctgaaaaga taaggaatga ttgtatatgc
    22681 atcagaagaa actggctctg gtcaatgatg tgaccggcta tggccgctgc agcatcgccg
    22741 tggccctgcc catcgtgtcg gccctgaaag tccagggctg cattttgccg acagctatct
    22801 tgtcggtcca tacgggcttc cacgactatt atctcgacga ctatacggat cacatgacgc
    22861 cgtatatcga gagctggaag aagaacggcc tgacctttga cggcatcagt acgggcttcc
    22921 tcggctcggc ccggcagatc gacatcgtcc tcgatttcct ccgcgatttc aagggacccc
    22981 atacattggc tatcgtcgac cccgtcatgg gcgacaatgg ccagctgtat ccgtcgtata
    23041 cccaggagat gtgcgatgaa atgcggcgcc tcctggccta tgccgacgta gtgacgccca
    23101 atgtgaccga agcctgtgag cttttgaacc tgccgtaccg gtcggaccgc gacttgagcg
    23161 aggacgattt cgccgccatg gccaaggcct tggccgacaa ggggccgcag ggcgtcatcc
    23221 tgacgggcat ccacaatggg ccgtacatcg gcaatttcat ctatgaccag gggaagatga
    23281 gctgggtccg ggccaaaaag gtcggcggcg accgcagcgg tacaggcgat gtctttacgg
    23341 ccatcgtatc ggcgtccgtc gtccgcggcg aaagcctgga acaggccgta cagaaggcgt
    23401 cggacttcat ctgccgcgtc atggcctata cggaagaact ggacctgccg cacaacgccg
    23461 gattggcctt tgaagaatat ctgaccacac tgagatgatg tctgccattc aggcagctct
    23521 aaataacaaa aaagaggata gggtattatg atcgtttaca ctgtagtagg acaaggacaa
    23581 tatgtagatc tcgcagatgg cttgaagatg aagcctgaag ggcagtggag catgtacgtc
    23641 aatctgacca accgctgtac ctgttcgtgt actttctgtc tgcgctccat gaagaagatg
    23701 gccgaaaatg aaacgctgtg gctgaagcgg gaaccgaccg tcgatgaagt gaagaaggct
    23761 ctcgatggcg ctccctggga ccacatcaac gaagtcgtct tctgcggctt cggcgaaccg
    23821 acggaacggc tggccgattt gacggccctc ttgaagtacg tcaaggaaac gcatcccggc
    23881 gtgaagaccc gcgtcaatac caacggcctg tccgacctgg cctatggccg cgatacggcc
    23941 cccgattttg gcggcggtat cctcgatacc atttccatca gcatgaatgc gtccaataag
    24001 gaacgctacc tggaactgac gcgcagcaaa ttcggcatcc agtcttatga cgccatgctc
    24061 aaattcgccg tcgactgtaa acagtacgtc cccaacgtcg tccttaccgt cgtcgaccac
    24121 gtcgaagaca gcgaagaaat caagaaatgc cgcgccatct gcgacaaact gggtctccac
    24181 ctccgcgtcc gcgtgtacga ggataaataa cgatgtaggg gct
//
LOCUS       DAWABZ010000016        23046 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_646_length_23046_cov_3.414684, whole genome shotgun sequence.
ACCESSION   DAWABZ010000016 DAWABZ010000000
VERSION     DAWABZ010000016.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 23046)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 23046)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23046
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_646_length_23046_cov_3.414684"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    13546..13555
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5099 a         6461 c         6276 g         5200 t
ORIGIN      
        1 agtattaagt ttgcagtttg tagtttttag tgaaggcttt taaatttaaa accattttct
       61 acaaactaca aactccgtac taaaaactat aaaataaaag aggctgtctt catatgacag
      121 cccctttttg aacccgtccc gccactagcc caagcggcct aaagatgtta aaatggatag
      181 gaaataagct gtttgctgtg tgaaggggga atctgcatgt ttacgtatta tatgccgacg
      241 aaggtatttt ttggaaaggg ctgtatcgct cagtccggcg gagtgctggc tgatctggga
      301 cgcaaggcgc tgctggtcac ggggcgtcat tcggccaagg tgaatgggtc ccaggctgct
      361 gttgtggaaa agctggatga attggggatt tcctggttcc tctttgatga ggtcgaaaat
      421 aatccgtctg tagcgacgat ccgcaaggcc gctgcattgg ccttggaaaa gggcgtcgat
      481 ttcgtcatcg gtatcggcgg cggttcgccc atggatgcgg ccaaggccat cgccctggtc
      541 tgcaccaatg agctggatga tgaacagttg tttaccggcc cataccgcaa gccgctgccc
      601 atcgtcgctg taccgacgac ggccgggacg ggcagtgaag tgaccaaggt cagcgtcctg
      661 accaatccca aggccgggac caagcagtcc atcaatacgc cgctcatttt cccggtctat
      721 gcctttgccg acccggcctt tacggacagt gtgcccgaag ccgtcaccat tgatacggcc
      781 attgatgcct tgtcccatgc catcgaaggc tatatgtcca cggcgtcgac gcccatgagc
      841 gatgtctggg ccgaagaagc catgcgtttc ctcggcgtcc atttgacgga cctcaaaggc
      901 gacgtttcgc ctgtcgtccg ggaagacctg ctctatggct cgaccctggc aggcatcacc
      961 attgcccaga cggggacgac catggtccac agcatgggtt atatgctgac ctattataag
     1021 ggactcagcc atggccgggc caatggcctg ctcttgccgg cgtacatgaa gctcatggaa
     1081 gaaacgatgg ccgataaggc ggaaaaggtc tgggctatcc tgggcctgtc cggtgccggc
     1141 gattttgcgg cccttatgca ggatctcatg ggcgataccg tcgatttgag cgacgaagaa
     1201 atcgaaaaat tcgtcaccct gaccttgccg acaggcggtg tccagcggac gccatatgcc
     1261 gtcacggcgg agctgctcac tcgtatttat cagcagttgg ctgggacaca taaatcgtaa
     1321 aagcgtaatt gccgtatgac gatagcgctt tttgagttcg cagtttttag tttttagttt
     1381 gtagtgaggg caaaaattta aaacccaatt tctacaaact acaaactcta tgctataaac
     1441 tattaaaagt aggaggattt tcatggcaga attattagcc ccggcaggct ctttagaaaa
     1501 tgtattcacc gccattgacg ccggtgccga tgccgtttat tttggcggga aaggcttcaa
     1561 tgcccgcaaa tttgctcata atctcgacga tgctgaaatc ggacaggctg tccggacggc
     1621 tcatttattt ggcgtcaaag tctacgtcac ggtcaacatc ctcatggccg atacggaatt
     1681 agcggaattg tccgcgtatt tacagctcct cgactcgctc gctgtcgatg gcatcatcgt
     1741 ccaggacctg gccgtagccg ctttggcccg gaaagtcgca ccggatctgc cgctccacgg
     1801 cagtacgcag atgactgtgg ccgacttgga cggcgtccgt ttccttgaag ccaatggctt
     1861 cacccagacc gtcttgtccc gcgaattgtc ggcgcaggaa atccgtttca tcgcttctca
     1921 gtcgtccatt cccattgaag tcttcatcca cggcgcgtcg tgcatggcct attccggcca
     1981 gtgcctgatg agcagtttcc tcggcggccg cagcggcaac cgcggtgcct gtgcccagcc
     2041 ctgccgtcag ccttatacgc tgctctgcga tggccaacct gtcatgaaac acgaggccta
     2101 tgtcctcagc ctgaaggatt tgaaatccct cgattatatc gacgacttgc tcgatgccgg
     2161 cgtcacgtcc tttaaaatcg aaggccgcat gaagggcgat acctacgtcc ggaccgtcgt
     2221 ttcggcgtac cgcagggtca tcgatgccca ttaccagagc ccgcagcagc gcaaggccgc
     2281 ccatcacgaa gcggcagccc tcctggaagg cgcctttaac cgcagttatc aggccgattt
     2341 cctgggagat tccgtcagcc gccgcacgtt gaccgaacgc aatccgtcgg gacggcagac
     2401 ggctgacgac atggccggcg gattgacgcg gaaactttcc atctatggcc acgtcgacac
     2461 ggaagaccag gggaacctgc gcctgacctt gtgggatgaa aatggccata ctgcctcggc
     2521 tgtagccgat ttccagcccg aaccggcccg ccagcggccg gcaacggccg attacgtcca
     2581 gggccagctc ggccgcctgg gcgatacgcc ctttgccctg gccagtgtga ccgtctggga
     2641 tgaaacgcgc atgatcccgg ccagtgtcct caacggcctg cgccgtcagg ccgtccaggc
     2701 tttgaccgag gccatcctgg cggattaccc gcggccggca gccggaaagg cccggcagcg
     2761 gcaggctccg gacaggcagc agggggacaa ggaagccatg cagctcgtcg tccgctgtga
     2821 cagtgtcgac ggcgtcgtcg ccgccgcgga ccagggtgcc gacgtcatca tctttggcgg
     2881 cgaatcgtac catcatcatc ccttcggcac taaggcctgg caggccgccg tccaggccgc
     2941 tcatgaacag ggcgcggcgc tgtgggccgg tacgccgcgt attgtccgcg aagagaaccg
     3001 gctggtcgtc cagaacgaac tggaacgggc catcacggcc ggcgtcgacg gcatctatgc
     3061 cggtgccatg gccattttcg ccatgatagc cgacttgaat attaccgtac ccgtccgggc
     3121 cgattggtcc ctcaatatct ttaacagcca ggcggcccgg gcctatgcat ccttgggctg
     3181 tacggccatt acggcgtctg tcgaggcgac gttgcgccag ttgaaagaaa tggtccgcca
     3241 gagcggctgt cccatcgaag ccgtcgtcca gggccgggcc gaaatgatgg tcactgagtc
     3301 ctgcgtcatt tcgtcctttg ccggcaaggg ccagaagaaa ggctgcccgg gcgtctgcaa
     3361 ccgcggtgcc tatgccctgc gggaccgccg ggacgaaacg ttccctgtcg tcaccgacca
     3421 gtattgccgc aaccacatcc tcaacagccg ggatctggat atggcgccgt attataaaga
     3481 cctgtgccgc gccggcatcg ccgccatccg catcgaaggc cgcggccgca cgccacagtg
     3541 gatcggccag caggtcagcc ggtaccgccg cctctgtgac ggtacggaaa cgatgctctt
     3601 aagcagggaa gaccagaccg tgacgcgcgg tcatttcttc cacggaatct tataacaaca
     3661 ggagtaatgc catttatgaa taaaagaagt atgaggattt tgggattcta ccaggtccag
     3721 aacatgctgg tccgcctggc cccgtcccgg ttgtctaaag aaatcgcccg ccgcctgaga
     3781 ccggtgaacg atggcgatgt cattgaaaag aacctgaccg atacggaaga agccgtccgc
     3841 tgcatgcaga cggaaacgag tacgcccttc ggcggcatcg tcgatatccg tccgtccctg
     3901 gaaaaggcca agcgcgaagt gaccctcgat tcccatgaat gtatcgatat ctggaataac
     3961 ctccagcatt atggggaaat ccggtcgttc ttcgccgaac ggggggctga gtatccccag
     4021 ctggccgaac aggcagaggt catcggcgat ttttcccagc tttctcattc tttcgccact
     4081 gtctttgaca acaaccgcca gatccgcgac aatgcgtcgc cggaattgat gcgcctgcgc
     4141 agccgtatcg tcgaactgga acggcagacc aagcgctatg tcaataatgt cctgcagaac
     4201 aaggaatacc agaagtattt ccaggatgcc ctggtcacag tgcgcaacaa ccgctatgtc
     4261 atccccatca agcaggaata ccgccatgcc tttcccggca tcgtccacga tacgtcggcc
     4321 agtggttcga cgctctatgt cgaaccgctg gccatcgtca atgccaacaa cgacctgcag
     4381 gccgcccgca ttggcgaaac gaaggaaatc gaacgcattt tccgccgcct gacggccaaa
     4441 gtgcagcagc agtacaatga cttgtatgac agcacgaagt gtgtcggtca gctggaattc
     4501 gcctttgcca aggcccagtt ggccttgaag atgaaagcct gccgtccggc tatttccaag
     4561 acgcgggaaa tcaggctcat ccaggcccgc catcccctca tcgacgccaa gcacgtcgtg
     4621 cccaatacca tcgtcatcgg cggcgattac cgcatcctgc tcattacggg ctccaacacg
     4681 ggtggcaaga ccgtttccat gaagaccctg ggccttttgg tcctcatgca tcaggccggc
     4741 ctgttcatcc cagtcggcga atcgtcggaa ctgcccgtct tccgcgacgt cttttccgac
     4801 atcggcgacg aacaggatat ttcccagaac ctgagtacct tttccagcca tatgaagcag
     4861 ctcatctaca tcctcaagca ctgcgggccg gaagacctca tcctggcaga cgaactcggt
     4921 tccggtacgg acccggccga aggcagtgcc atcgccattg ccatcctcga tgccttttac
     4981 cagaaaggct cgtatgtcat ggtcacgacc cattacaatg acctcaagaa ctatgcctat
     5041 aatacgccgg gcatcgaaaa cggccacgtc gagttcgata tggaaacgct gaagccgacg
     5101 tacaagctgc gcatcggctc ggccggcagt tcccacgcct tcagcatcag cgaacgcctg
     5161 ggcatgccca aggatatcct ggaaaaagcc aaagacctgc gcagcaaggc tcaggacatg
     5221 aatatggaaa aagtcctgac ccagctcaat gcacaggcca aaaagatgga cgaagaacag
     5281 gcggaactgg aataccgcct gcgcgaagcg cggaaactgg aagacgattt gcgcaaagaa
     5341 aaggacaagg tcacgtcaaa gcggcaggat atcatcgacg ccagccgccg cgatgccgtc
     5401 gacctcaagc gcaacctccg cgtcgaagcc gaaaagatta tccgcgagct gaaggcccag
     5461 tccaaggagg ggacggaccg ggaaaaggcc aaggccatcg acaaggcccg ccgggctatc
     5521 cagcagattt ccctgcctga agcggaaaag ccccagcgcg accctgtcga cctgtcgaaa
     5581 ttgaaagtcg gccagcaggt cttcgtcaac aacctggaca gcgtcggcac ggtcgaagac
     5641 atcggcagca aacagctgac cgtctccgtc cggggcatga cggtccgggt caaattcaaa
     5701 gacgtcagtg cgccgtacct ggacgaattg aagaaagaac aacaggccga aaagaaagcc
     5761 gctgccgcct cgtcgtaccg gcctatccgc acgtccagcg tggccacgga attgaacatc
     5821 atcggcaaga ccttcagcga agccctgccg gaagtagaac gcttcctcga ccaggccctg
     5881 gctgccggct tcagtccggt caagatcatc cacggcaagg gcagcggtgc cctgcgccgt
     5941 cagatccacg ccttcctcga cgaccagccc ttcgtcaaga aataccagct cgacgacgta
     6001 gaaggcggtg gtgccggcgt caccctggtc tatttctaag gaaagtgggg ggctgtcgca
     6061 cgaaggtttt tgccatcatg caagacccct cttactttat agtttgtagt atggagtttg
     6121 tagtttgtag aacggagttt atagtttgta ggggacgccc gtgggcggcc cgcccctaca
     6181 gaaaaaggag ctgtcttcgt acgacagctc cttttttcat gtaatatttc ctatgctgac
     6241 tacgccatca ccatggtcga tgaaatcgaa aagggccatc acatcaatca gcgcatcagc
     6301 gtcgtcgggg cctaagtatt cctggatctc ctaaaaaaga agcagcccac cttgtcgtaa
     6361 ccaccgtatc gcagtggctg cggcaacatg agccgataga agtcgtcttc aacgttttta
     6421 ccgaggaaga ccggataata tatgaacgag aactagtgga aaaagggaat agtagattgt
     6481 gcggtagggg tcgccgcgtg ggcgacccgc tgggcatttg gaaacgctct ccccgagttg
     6541 gaaaatcgct catattctgg ggcgggcttg ccacgtgcaa gcccctacta gaacagcatc
     6601 tggaagtgga tgcgggtcat ttcgtggggg tccgggctgc cggtcttggc ctggctggcg
     6661 aaggactgca tgacgctgaa tttcaggttc ttggcgacga tgtagtcggc accgatgccc
     6721 cagctgcgct ggttgtccat gtcgctggag aagcgccacg tgaaggggtt gccgcccatg
     6781 aaggagccgg cgatatattt ggcaaaatag tcttcacccg aactgtggtt ggactgatga
     6841 aggcggttgt agtagaaacc ggcttcggaa cgggcggcct tgccgccgcc gaagaaggcg
     6901 tcgatttcac cgtagcccgt cttcaggcca tccgtactga agtgatccga atgggcggcg
     6961 tcggaaatcg gcttcaccgt atcgacgccg ggtttcttgc cgtccttgaa tttgccgtag
     7021 cctgtggtga tggccaagtt atcgttgtga tagcgcagtt cggcgccgtc gagggcatcg
     7081 aagtactgca ggccgtaggc gtcgcggccg atgcggtaga cgtcattgcg gccgacgctg
     7141 agttcccagt tcttgctgcc gaagttgtag tccactttgg ccaaatcgac atagggatta
     7201 ttgccttcgt cagaagcggc tgccagggta cagactactt gtttcttcac gaataaaaga
     7261 ccttctttct tctcattctt tttgcgtatg cagaaaagtg cataaaatta tacattgcct
     7321 ctagtatact acgatgtgtt atgactgtgt gtcaaatgag aaggaagttt accttttctt
     7381 tataatttta cgtgactgaa gacttcgcca atcgggtcgt ggaggaccag gtcggcggcg
     7441 ctgtccatgt cggtgctcga cttgttgatg aggaccagtt tatggccgcg gtagtagcgg
     7501 accaggccgg cagcggggta gacgacgagg ctcgtgccgc cgatgatgag catgtcggcg
     7561 tggctgatgt agtacaggga ctgttcgatg acgtcgttgt ccaggccttc ttcatagagg
     7621 acgacgtcgg gcttgatggt gccgccgcag gcgtcgcact tggggatgcc tgtggaattc
     7681 ttcatgtatt cggcgtcgaa gaatttgttg cagtgcatgc agaagttgcg gtggacgctg
     7741 ccgtgcagtt ccaggacatt cttactgccc gctttctggt gcaggccgtc gatgttctgg
     7801 gtgacgatgg ctttcaattt gccggctttt tccagttcgg ccagcttgat gtgggccatg
     7861 ttgggctggg cgtcgagggc cagcatcttg tcgcggtaga agcggtagaa ttcttccgtg
     7921 ttgtccatga agaaggagtg gctgaggatc gtttcgggcg ggtatttata gtgctggttg
     7981 tacaggccgt cggtgctgcg gaaatcgggg atgccgcttt ccgtcgatac gccggcgccg
     8041 ccgaagaaga cgatgttatc ggaatttttt accatgtcga cgaatttttc aatatttgtc
     8101 atcgtaatta cccctttcat acattgtagg tgtctttcaa taactgcagg cccgacgggg
     8161 tgcggaatac cttgtcgcgg aaggtggcaa tggcttccgg tttcagctgg tcttcatagg
     8221 cattgaccag gaaatcggct tccaggagta tctggtagtc cagtccgtcg acaccgtcgt
     8281 aggtgtgatg gtgggcgatg aggaagcaga cccggtcgat gagcttttcg tcagcaccga
     8341 gttcttccag gatagccctg gccggggcgg gtccttcgat ttcctgatat ttgcccgacg
     8401 aggaaccgta tttttcttcg gctgcgtgga tgccgatgtc gtggaggacg gcagccgtgc
     8461 gcaggatgtc ctgctggtct tcgggcagtc cttccaggcg gccgatggtg tcggcatagg
     8521 cgtagacctt gaggaaatga tggatgcgcc gggcatcgcc gtggtcgaag gcgatagcct
     8581 tctgtattac ttgttcaata gctgtcatgt aaatccctgc ctttctgttt cattatacta
     8641 cagggccggg aagataggaa cggcctgata caaaccatcc atagcctatt gacagattat
     8701 cacatcatac cgtatccttt ttataggact ttttttaatt tttatttata tttttatttt
     8761 tatggtaagg atgtgattct atgattatgg ctgtcctgaa ctacctcgat gatgtccttt
     8821 attatcctat cttaatggtc atcctcattg cagccggcct tattttttcc tggaagacgc
     8881 gctgtgtcca gctgcgcctg ttcccggaaa gtatccgcgt cgtcatggaa aaaccgacgg
     8941 aagccgggaa agtatcgtct ttccaggccc tcatggtttc gacggcgtcc cgtgtcggta
     9001 cgggcaatat catcggcgtc tcgacggcaa tctgcctggg cggtcccggt gccgttttct
     9061 ggatgtggct tctggccatc atcggcgggg ctacggcctt cattgaaagc acgctggccc
     9121 agatttacaa gcgccgcaat cccctgggcc acagctacgg cggtccggcc tactacattg
     9181 aaacggccat gcacaagcgc tggctcggcg tcctcttcgc cttttccctg atcgtgactt
     9241 atgccggcgg cttcaacctg ctctgctcgt tcaacatgca gtcgacgttc ctggtctaca
     9301 gcttctacga cccgacgacg acgccgtgga tcatcggcgc catttttgcg gctctcgtcg
     9361 cctactgcct catcggtggc ggccagcgca tcatcaaggt cacatcggtc ctggttccgc
     9421 tcatgggcgg catctatatc ctggcagcgc tcatcgtcat cgtcttccac attaccagcc
     9481 tgccgcaggt cttcgccatg atcttcgccg atgccttcga cttccagtcc atcctcggcg
     9541 gcgtgtccgg ctcctgcatg gtctacggta tcaagcgcgg cctctattcc aacgaagccg
     9601 gtatcggttc ggcaccgaat gcagcagccg cagccgatgt cagccatccg gtcaaacagg
     9661 gcctggtcca gatgctgtcg gtcttcatcg ataccctcct ggtctgcagt gctacggctt
     9721 tcatgggcct cttctcgggc gtgcccatca ctaaggaagt agccggtgcg gcttacgtcc
     9781 agaatgcagc tacggccgtc ttcggcggct tcggtccgct cctgattacg atctgcatgc
     9841 tgctcttcgg tttttccacg ctcatcggca acttgttcta tgtcgataac tgcatccgct
     9901 tcatccacaa gggcgcgccg tcccacggct tcgtcaatac tttccgcatt gtctgcgtcc
     9961 tcgtcgtctt cgtcggcgcc ggcatgtcca tggccgctgt ctgggatatt gccgatatcc
    10021 tcatggccgt catgtgcttc atcaacatcc cggcctgctt catcctgggc aacacggccg
    10081 tcaaggccat gcgcgactac cagcagcaga agaaggaagg caggaaccct gtcttcaagg
    10141 cggcttccat cggcattgat gaaagtacgg tccagtattg gaagtaagcc tcttgacaaa
    10201 tgggggataa gcctttacaa tacgtctatg tacaagtaat tgtcacaaaa aatgaaaagc
    10261 gatgaacaag acggccagtg tcgcctgtgc cttttcagcg aaccggggat agtgagagcc
    10321 cggcaaaggc cgtatgctgg cggatcactt gggagctgca gctgcgaacc agctgccggg
    10381 tcgcgtcgtt attgcgcgtc aaagtggctg ctccggcagt caatagggtg gtaccacggt
    10441 gattacgtct tcgtcccttt ttgggacgga gacgttattt tttttcacag attcagagga
    10501 ggaaacacaa tggattacgg taagacttta catctgcctc agaccgattt ccccatgcgc
    10561 ggcaacttgc cgaaacggga accggacttt ttgaaattct ggcaggacaa caagatttat
    10621 gaaaaacgct tgaagaaacg ggacggtgcg cccaagttca ttcttcacga cggtcctccg
    10681 tatgccaatg gcaagatcca catcggccat gcattgaata aagtattaaa agacattatc
    10741 ctcaaatata agacccagca gggctgctat acgaaatacg ttcccggttg ggatacgcac
    10801 ggcctgccta tcgaacacgc cgtcatcaaa gatacgggcc tgaaccgcca cgaaatgacc
    10861 cccctcgact tgcgtgccaa atgccgtgac tacgccctgg cccgcgtgga agaacagaag
    10921 aaagacttca tccgcctcgg cgtcctcggt gactgggacc atccgtacct gacgctgcgc
    10981 aagcccgtcg aagtcgctca gatcggcgtc ttcggggaaa tggccaagaa aggctacatc
    11041 tacaaaggcc tgaagaccgt ttactggtgc ccgcactgcg aaacggccct ggctgaagct
    11101 gaaatcgaat acaaggatga taaatccttc tccatttacg tcaaattcaa gtccgtcgac
    11161 ctcagctgcc atatgccgaa aggtgccgat ccggacaaag tcttcgccct catctggacg
    11221 acgacgccgt ggaccattcc ctgcaacgtc gccatcagtg ccaacgaaaa ctttgaatac
    11281 gtctgggtac gcatcggcga cgaatacctg ctcatggcca aagacctggt cgaaccgacc
    11341 atgaaagccg gcaaagtcga cgactacgaa gtcctgcccg acgtcatgac gggcaaacag
    11401 ctcgaaggcc tggtcttcaa gcatcccttc tatgaccgta aagtccccat catcctcggc
    11461 gaccacgtca ctctcgaagc tggtaccggc ctcgtccata ccgctccgga ccatggccag
    11521 gacgactttg atgtttgcaa aaaatatgct gcctggggcc tcaagccgct gggcacagtc
    11581 gacggcacgg gccgttacac cgacaaggtt cccggctttg aaggccagtt cgtcttcgat
    11641 acgaacgtac cggtcatcaa gaaactggct gaactgggcg ccctctttgc caagagcacg
    11701 ttccgccatc agtatccgca ctgctggcgc tgcaaggaac cgatcatcta ccgtgctacg
    11761 gaacagtggt tcgcttcggt cgacggcttc cgccagaaag ccctcgatgc catcgacacg
    11821 gtcaaatgga ttccgtcctg gggccatgac cgcatttaca atatgatcca cgaccgcggt
    11881 gactggtgca tttcccgtca gcgtgtctgg ggcgtgccga ttcctatctt ctactgcgaa
    11941 gactgcggcg aacacatcat caacgatgaa accatcgctc atttgcagaa gatgttcgcc
    12001 aaagaaggct ccgatacgtg gtggatgcac gacgtcaagg aactcatgcc ggaaggctat
    12061 aaatgcccgc actgcggcgg cacgcacttc cgcaaggaaa cggatatcat ggacgtctgg
    12121 ttcgacagcg gctgcaccca tcagggcgta ctgaaaaatg acccggacct cgattatccc
    12181 tgcgaaatgt acctggaagg ctcggaccag catcgcggct ggttcaacag ctctctgctc
    12241 acgtcggttg ccgtcaacgg ctatgctccg tacaaatcgg tcctgaccca cggcttcacg
    12301 gtcgacggcg aaggccgcaa gatgagtaaa tccgtcggca acaccgtcgc tccccaggaa
    12361 gtcatcgaaa aatacggcgc tgacgtcctg cgcctgtggg tatcgtcggc tgactaccag
    12421 ggcgatatcc gcttgtcgcc gaagatcctg aaacagctct ccgacgtcta ccgcaagatc
    12481 cgcaacacct tccgctacct cctgggcaac ctggccgact tcgatccggc taaggatacg
    12541 atcgcttacc aggacatgac ggacctcgat aaatgggctc tcctgcgtct ggaacaggtc
    12601 tttgaagaag tcaccgatgc ttatgaaaac taccagttcc acgtcatgta ccacgctatc
    12661 cacaacttct gcaccgtcga tttgagcgcc atgtacctgg atgtcatcaa agaccgcctg
    12721 tatacggaaa aggctgattc ccatatccgc cgcagcgccc agtcggctat gtacatcatc
    12781 ctcgatacgc tggtcaagat cgtcgcaccg gtcctgtcct tcacggccga agaagtatgg
    12841 cagaacatgc ccgctgtcga aggcaaggaa gaaagtgtcc tgctcaccga ttggcccaag
    12901 gctcacaaag aatatctcga cgctgacctc gatgcccgct gggctcagtt cctgtcgtac
    12961 cgccgcgatt tgacgcgtgt cctcgaaggc gcccgtaagg aacacaagat cggccatgcc
    13021 ctggacgcag ccgtcaccat ctatgccgat ggcgaagact acgatttcct cagccagtgg
    13081 caggatcagc tggccacgct gctcatcgtc agcgaagcca aactggtcaa aggcgaagcc
    13141 ccggctgaag ccgttgccgg tgaagaccac ggcgacatga aagtcgtcgt cgctccgtcg
    13201 acagctgaaa aatgcgaacg ctgctggatc cacagcgaca ctgtcggctc ggatccgaac
    13261 catccgaccc tctgcgcccg ctgcgctgcc gtattaagtg aataaaagtt tgatatatga
    13321 aaaaagggac tgtcacacgt gcgacagccc ctttttttag cagtcagcgg ctagcagtta
    13381 gcggatggtt ttaaatttag tgctatccac taaaaactac aaatgctgag aaatcaacct
    13441 ctcagaccgc ctttggcggc cagctctctt atgaggagag cctttcgtca aatatcaaac
    13501 tattttcacg aatcaaatgc ggcttccata gcagcggctt tttccnnnnn nnnnnctcct
    13561 atgaggagag cctttcgtca aacatcatac atcaaaggca aagaaaacct acgtgataac
    13621 aaagagcttt acatgagatt tctttttttt attgaaaatt tacggctttt gctttataat
    13681 aaacaagtgt attcatacat tatacatttg taacatggaa ttagggtagg aggttttatt
    13741 tatgcatagt ttatggaatg cattgaaccg catcagcctg atcaagcaga tcatcatcgg
    13801 cttggtcatc ggtatcgccg tggctatggc tttcccggca gcagggaagg aactggcctt
    13861 gctcggcgtc atcttcgtcg gggccttgaa gggcatcgcc ccggtactgg tcttcgtcct
    13921 ggtcgccgct gcactgagcc gcaaacagga agaccacgaa tcgaacatca aagatgtcat
    13981 catcctgtat ctcatcggta cttttttagc ggctttcttt gccgtcatga tcagtttcgt
    14041 cttccccctg acgctgcagc tcgacgtatc ggctgccagc aataaggcac cggaaggcat
    14101 tgccgaagtc ttgacgacga tcctcaagaa catcgtcgac aacccggtcc acgccctgat
    14161 gacgggcaac tacatcggta tcctcggctg ggcctgcctg ttcggcgttg ccgtccgccg
    14221 cgtctccgaa tcgtcgaaag ccgtcctcga tgactgtgcc gacgctgtca cgacctgcgt
    14281 ccagtggatc atccgcctgg ctccgctcgg catcatgggc ctcgtcgccg actccgtttc
    14341 gtccaacggc atcggtgccc tcatcggcta tgctaaattg ctctgtcttc tcttagggtc
    14401 gatgctgctc ttcgccttcg tcatcaaccc gatcatcgtc tacgccaaga tccaccgcaa
    14461 tccgtacccg ctggtatgga aatgcgtcaa gacgtctggc gtcacggcct tctttacgcg
    14521 cagctcggcc gcgaatatcc cggtcaacct gtccctggcc aaggaactcg gcgtcaatcc
    14581 cgatacgtat acggtcacca ttccgctcgg tgcgaccatc aacatgtccg gcgcagccat
    14641 caccatttcc atcatgaccc tggctgccgt ccacaccctg ggcatttccg tcgacttccc
    14701 gacggccctc ttgctcagcg tcctttcggc catcggcgcc tgcggtgctt ccggcgttgc
    14761 cggcggctcg ctgctgctga tccccatggc ctgcagcctc ttcggtatct cctatgatat
    14821 cgccatgcag gtcgtcggcg tcggcttcat catcggcgtc ctccaggact cgaccgaaac
    14881 ggccctcaac tcgtcgacag acatcctctt cacggcagct gccgactttg ctcagcgcgg
    14941 ctatccggaa cacctgggtg gcggccacga agtcagccat caagggtagg aacgcccctt
    15001 gggcgttccg caggccgcag ttcgcagtcc gcggtccgcg tgtagggcgc cacggattat
    15061 gacgaataat gcaggccgcc gttcgcatgt aggggcgcca cgtggggcgc ccgccacgga
    15121 ttataatgaa taatgcagtc cgccggccga aaaaggaccc tcgcatgacg gtattcgtca
    15181 tcatgcgagg gtcctttttc ttggtcaaaa acaacctctc agaccgcctt cggcggccag
    15241 ctcccctata aggggagccg ctagccacgg gccactaacc actaaaaaag ggcctgtcgc
    15301 acgaaggttt ctgccatcgt gcgagggtcc tttttcttgg tcaaaaacaa cctctcaggc
    15361 cgctttcggc ggccagctcc cctataaggg gagccgctag ccacgagcca ctaaaaaagc
    15421 gttgtccgtc aggacaacgc ttttttctac cttatttccc gctttcagcg atgcagtcat
    15481 tgaggtcagc aatgagtgcg tcgacgtcga tgccgtgggc accagcgccc tggccgatgg
    15541 tttcaaagtg agcagccatg cagccaatgc agccgaggcc gtactgctgg aagatttcaa
    15601 tgatctgcgg atatttctga acggcttcaa tgatacccgt gtcttttgta atctgtgcca
    15661 ttatttttat tcctccttgt caattcgtat ccgtaccgtt ttgaaggaac ttccggattg
    15721 catctattat atcatacttt ttttcatttg tgcgaggaaa tacttttttc ctccaagagt
    15781 tggccgtcgg gcaggaagct gcggaaggtc aggctgtcgt ccgtcgcttc cagggtcatg
    15841 taattgtccg tttccggctg gggtgcgacg tattcgtcga ggctgtgctg tttccacaat
    15901 cccggatact ggacgttgcc ggcgacgccg gtgatgaggt acagcgggcc cgattcatcg
    15961 cgctggaagt tcctgatatg gccccggtcg cggtaggtgt ggagatgggc cgagaggacg
    16021 gcatcgacgc cgtactggtc gaagaggggc atccacagcc ggccttcggg cgagaagcct
    16081 tcttctcggg gctggggccg gttggcgaag ccgtactgga gcgggtcctt gtgtatgagg
    16141 acgattttcc atttcttcgt cgtcttggcc atgtcttcct tgaaccaggc cacttcgtct
    16201 tcgtcgaggc tgggctcgaa gtcagccagt tcctgggact gcgtgttgag gacgacgaag
    16261 tggacgtcac cgtagtcgaa ggagtagaac tggttctggt atttttccct gtcgatacga
    16321 ggcagggcga agaggtgcag gtaggcttcg ggcatgcgga ctttccagtc cttgtcatac
    16381 gtttcgtggt tgcccaggag ggggacgacg gggatgcggg cgatcatgtc gccgacgacg
    16441 tcgaaccagg cgttccactg gtaatggtcc tggccgttgt cgacgaggtc gcccatgttg
    16501 atgaagaact gggcgtcctg gttgcgctgc caggcaggtt gggccgtggc ggcccagacg
    16561 ctgtagtcgc tggactggga atccgggaag atgagggcct tgaagtcatg gccctgggcc
    16621 gtatggaagg actgccagtc actgcgtttg tcgccggcac cgacgcggta ttcataggcc
    16681 gtacccggtt ttagatcgct gacggccgcc gtgtggatgt acgtcgtcac gccatcgtcg
    16741 ctgaaggcgt cactggaagc ggggagctgc atgagggaat cgtcgtcgcc ggcttggcgg
    16801 tattcgacga caggattctc ttcggcgtag tccgactgcc acatgaaggt ccgcgacgtc
    16861 gtgctgtcgg ctgtgatgac ctggcggatg ttcgaggcat cgacggcatc cgtcgacagg
    16921 tagtgggccg ccgcggcttt ggtggccttg acgccctgtc tggcgacggt cttcgtaccc
    16981 ggcagccagg tataggcact gacggcggcg atgacggcag cggccaggga aaaggcgatg
    17041 atttttttat atttggcttt catagaattc ctcctgtttt ttttgatata agtcattata
    17101 agacatggag tcaactccaa gtcaagcaaa aaagaaaaac ttgacaaggc cttcgcttcg
    17161 atataagata gatgcatcaa aagtgataga gaacgtccat gaacgggagc aagtaccgtc
    17221 atgataccct tgacagcgag ccggtggcag tggaagtccg gcgaagaggc catgagcggg
    17281 aatgggcccg ggagatgcag gctgaacgcg agtaggtcct gccggaactt tcaccgttac
    17341 caggaaagaa tgcatctacg tgtgcatatg agccgggcag ttttgccaac cagggtggta
    17401 ccgcggaaat gactttcctt tcgtccctaa tgtgagggat ggaaggattt ttttattgaa
    17461 cagggaggaa tccatcatgt ctattatttt ttctggcatt cagccgagcg gcaatttgac
    17521 tttaggcaac tatttaggcg cactgcgcaa tttctcgaag atccaggacg gcaacgaatg
    17581 ttattactgc gtcgtcaacc agcatgccat cacggtccct caggatccgc agcttttgca
    17641 tgaacgtacg cggaaactgg ctgccatcta cctggcttcc ggtctggatc cggaaaaatc
    17701 gacgctcttc gtccagtcgg aagtcccgga acacgcactc ctgggctgga tgatgattac
    17761 cttgtcctat gtcggcgaat tggaacgcat gacccagtat aaggacaagg cttccaaacg
    17821 gggcgattcc atcccggccg gcctgttgac ctatccgccc ctcatggctg cggacatcct
    17881 cttgtaccag accaacttcg tccccgtcgg cgacgaccag aagcagcaca tggaaatcac
    17941 ccgcaacctg gcccagcgct tcaaccgctt ctacggcgaa gtcttcacca ttcccgatat
    18001 ctacctcggc caggacggca cgcgcgtcat gagcctccag gaaccgacca aaaagatgag
    18061 taaatccgac gacaacacga cggcgacgat ttacctcctg gacgaaccga aagccatcga
    18121 aaagaaaatc aagcgggccc agacggacag tgaaaactcc gtccattacg ataaggagaa
    18181 caagccgggc atttccaacc tcattgaaat cttctcggcc ctgaccgacc agacccacga
    18241 acaggtcgaa gcggcctacg ctggcaaggg ctacggcgcc ttcaagaaag acgtggccga
    18301 tgccatcatt tcgacgctgg aaccgatcca gcagcgttac cgccagctcg tcggcgaccc
    18361 cgaactggac gccatcctcg acaagggcgc tgccaaggca cacgcaaagg ccagcgtcac
    18421 ttacgaaaaa gccgccaagg ccatgggttt ataccggaag taaaaattaa aaatatggca
    18481 acacaactta atactttatt ttaaatatta tattgacata acaaaaataa taaagtatta
    18541 tactttacac atcgaaaaaa taaataaagc agaggcaacg gtgccatcag acactgtgct
    18601 tctgcttttt ttattttgta aagtcatctg tgttataaag ggggagtgcc atatggcagc
    18661 gaaaaagtct agttttgaca aaggactcaa tgcctgggac gtcctggtca tcgccttcgg
    18721 ggcaatgatt ggctgggcct gggtcgtatc gacaggggat tggatcaccg gcggcggtgt
    18781 cctcggcgcc atgctggggt tcgccatcgg cggcgtcatg attttctttg tcggccttac
    18841 ttatgccgaa ttgacatcgg ctatgccgca gtgcggcggc gaacacgttt tcagttataa
    18901 ggccatgggg cctgtcggtt ccttcatctg cacgtggatg atcgtcctcg gctatgtcag
    18961 cgtcgtctgt tttgaagcct gcgccttgcc gacgatcctc gtctacttgt tcccggattt
    19021 cctcatgggt tacttgtaca ccatcggcgg cttcgacatc tatgcgacct ggctggctgc
    19081 ggccgtcatc atcgctgtcg ccattacggc tatcaacatc atcggtgcca agacggctgc
    19141 ccgcctgcag accatcctga cggccatcat cggcggcgtc ggcatcgtct ttgtggctgc
    19201 ttcggtcctg accggttcga cggccaacct ggacggccag ctcttcatgg gcgctacgac
    19261 gtcggaaatg gccaagggta ccctggctat cgccgtcatg acgccgttct atttcatcgg
    19321 cttcgacgtc atcccgcagg cagccgaaga aatttccgta tccttgcaga aaatcggccg
    19381 tatcctgatt ttgtccatcg tcctggcggt tctcttctat gccctcatca tcttcggtgt
    19441 cggctatatc ctcaaccaga gtgaaatagc cctggctatg aaaggcgtcg gcagcctggt
    19501 cacggccgat gccatggctg tcgcctttgg cagttccatg atggccaaag tcctcatcat
    19561 cggcggtatc tgcggcgtcg tcaccagctg gaattccttc ctcatcggtg gcagccgtgc
    19621 catgtattcc atggctgaat cgtacatgat tccgcatact tttgctaaat tgcacgacaa
    19681 atataagaca ccggtcaacg cgctcctgct catcggcgtc ttgtccattg tcgctctgtt
    19741 cttcggccgc aagatgctgg tctgggtcgt cgatgccggc aacttcggct gctgcatggc
    19801 ttactgcatg gtttccattt atttcctgat cctgcgcaag aaggcgccgg acatggtccg
    19861 tccctataag gtacgccact ataaattcgt cggtgccatg gctgtcctca tgtcggcctt
    19921 catggtcgtc atgtacatcg tccccggttc cggtgccgcc ctggctccgc aggaatgggg
    19981 catggtagcc ggctggtccc tgctgggcgt catcttcggt gtatactgca aggccaaata
    20041 cggcgaacgc ttcgctaccc atatcgacgt cgccatggat acggaagtca gggaagcggc
    20101 cctgccgtcg agtgaactga agacggtcaa attgtaaatg ccttctcaaa gattgtcttt
    20161 aaaattagaa agttaaaata acacagattt ataaaaataa aagcaaataa catattgaca
    20221 aagaaagtgt aaagtattac actatattca acaaagaaaa taacggagac aagaggcaag
    20281 ggagcctgat ggaacatcag ctttccttgc ctctgttttt gtttggggaa ttctttgtta
    20341 gttatcattt ctttataaaa tcatcagttt agggggagtt acgaatgaga ttacgcgatg
    20401 taggattgac caaagaagaa atcaaagcca aagtagacaa gtacatgatc gaaacgtacg
    20461 aacgcttcga catgatcgct gaacgcggca agggcatgta cgtctacgat gaaaatggca
    20521 cgccgtacct cgatttctac gctggtatcg ccgtcaacag tgccggcagc tgtaacgata
    20581 aagtcgtcct ggccgtcatc gaccagtgca tggacatcat gcagaccttc aactatccgt
    20641 acacggtccc gcaggccctg ctggctgaaa aaatctgcac gaccatcggc atggacaaga
    20701 ttttcttcca gaactccggt gcagaagcca acgaagccat gatcaagatg gcccgtaaat
    20761 acggcgtcga acactttggt cccaacaaat acgaaatcat tacggcaaaa gaatccttcc
    20821 acggccgtac ctttggttcc atgagtgcta cgggccagcc ggacaatgcc tgccagatcg
    20881 gtttcggcgc catgacaccg ggcttcaaat atgctgaatt caacaatttg aaagcctttg
    20941 aagacgccat cacggaaaac accatcggca tcatggtcga accggtacag ggtgaaggcg
    21001 gtgtccatcc ggcaacgaaa gaattcctca caggcctgcg caaactgtgc gatgaaaaag
    21061 gcctgctcct cctcctggac gaagtacaga gcggctggtg ccgcaccggt gctgtcatgt
    21121 cctacatgaa ctacggcatc aaaccggata tcgtttccat ggcaaaagcc cttggcggcg
    21181 gcatgccgat tgcagccatc tgcgcccgcg aagacgtttc caaagccttc accatgggtt
    21241 cccacggcag taccttcggc ggccatccgg tctgctgcgc cgctgctttg gccgaagtca
    21301 acgaactgtt ggacaaagac ctggccggca atgccaagaa gatgggggca tacttcctgg
    21361 aacaggcccg cgaaaaactg ccgcatctga aggaagcccg cggtcagggc tgcttcctgg
    21421 ggcttgaatt cgacgacaag atcaacagcg tcgacttgaa gcacaaatgc ttcgacaaac
    21481 acatgctgtc gacagctatc ggcgaacacg tcctgcgcct ggtaccgccg ctgatcctcc
    21541 agaaggaaca cgtcgacaag gccattgcca tcatcaagga atcggtcgaa gaattggcaa
    21601 aataagtaca cgtaactgag gggtatgaga aggtgtctgc cagaattgag gggttggcag
    21661 gcaccttttc ttaaaaaaag ggggaaacga aatgaaacat ttcgtcatca ctatgggatg
    21721 tgaattcggc agcggcggac cggaaatcgg caagatgctg gccaagtcgc tgggaatcga
    21781 atactacgac cgggatctcg tagataaagt cgtggaaaaa atcggcgtag aaaaacatct
    21841 cgtagaagaa gcagataata agaatttcgt tccgtacggc attgaaacgt cgttggggac
    21901 gcgctatgcg aatctgtcga ataaagtcat ttacacgcag ttcgatgtca tccgcaagat
    21961 ggccaaaaca tcgtgcgtca tcatcggccg gtgcagcgac tacatcctga aggggcagga
    22021 tgatgtcgtc aacgtcttcg tctatgcacc gacggaagta cgcatcaaga ccatcatgga
    22081 aaagatgaat ttatccgaac gccatgcggc agaagtcatc cgggattatg acaatgccct
    22141 ccatcgccgg tataaataca tcaccggcac ttatcgcggc gaccgtcaca accgccacct
    22201 gctggtcgac agcagcgtcc tgggctggga aaagacggcg aaattcatca aatccttcgt
    22261 cgaaatgtgt tttgaagaga cttgaggggg tatgaaagat ggctacaaaa aagtctgatt
    22321 ttgataaagt cctgagtgcc tgggacgtcc tggtcatcgc ctttggcgct atgatcggct
    22381 ggggctgggt cgtatcgaca ggcgactgga tcatggaagg cggcgtcctc ggcgctatgc
    22441 tgggcttcgt cattggcggc atcatggtct tcttcgtcgg cctgacctat gccgaactga
    22501 cgtcggccat gccgcagtgc ggtggcgaac acgtcttcag ttataaggcc atggggccca
    22561 ttggttcctt tatttgtacg tggatgatcg tcctcggcta tgtcagcgtc gtctgctttg
    22621 aagcctgcgc actgccgacg atcgtcgctt atatttatcc tgatttcctg acgggttata
    22681 tgtataccgt tgccggcttc gacatttacg cttcctggct ggctgtggct atcctggttt
    22741 ctgtcttcat tacagccatc aacatcgtcg ggaccaagac ggctgccatc ttgcagacca
    22801 tcctgacggt catcatcggc ggcgtcggta tcctgctcat cgccgcatcg gccctgaccg
    22861 ggacgacggc caacctcgaa ggtcagctct tcatgggggc tacgacgacg gatatggtca
    22921 aagggacttt ggccgtggct gtcatgacgc cgttctattt catcggcttc gacgtcattc
    22981 ctcaggcagc ggaagaaatt tccgtatcct tgcagaaaat cggccgtatc ctgattttgt
    23041 ccatcg
//
LOCUS       DAWABZ010000017        22685 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_650_length_22685_cov_3.250508, whole genome shotgun sequence.
ACCESSION   DAWABZ010000017 DAWABZ010000000
VERSION     DAWABZ010000017.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 22685)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 22685)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22685
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_650_length_22685_cov_3.250508"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4906 a         6588 c         6133 g         5058 t
ORIGIN      
        1 gcctggcagc ccataagagc ggccaggatt tcggcgtcga cagccgtcta ggcccagccg
       61 acccggccct ggcttcgtcc ctggccagcc atctcgggac gaccgtcgcc atcaccggcg
      121 ccgtcgatgt cctttccgat ggctgccaga cggccgaaat ccgcaacggc tgtcccctgc
      181 tgacctatgt gaccggtacg ggctgcatga cgacgtccct catcgcctcc tttgccgccg
      241 tggccgaccc gttcacagcc gcagccggcg gcatcctggc catgggcatc ggcggcgagc
      301 tggccctggc aggggagggg aagaatgggc cggggacctt tcacgcccgt ctcttcgatg
      361 ccttttatca attaaatgat gctatcttac aaaaatatgg aaaggttgta gtacatcgtg
      421 aataatactg aactcaagaa aatgacagtg acggcgattt tcatcgccat cggcgtcctc
      481 ctggcgcctt tcgtatcctt tcccttcggc ggtgcccggg tcttcccgct gcaacacctc
      541 atcaatgtcc tcttgtcggt cctcgtgggc tggcgctatt cgacggccgg tgctttctgc
      601 atttcatcca tccgcattgc catgggcgtc ggtaccgtcc tggcctatcc cggcagcatg
      661 atcggcgccg ctttatccgg cgttttgtat aaaaaaacaa attccatctg gggtgccgtc
      721 atcggggaaa tcgtcgggac cggccttctg ggcggcctca tcgcctatcc tgtcgccgct
      781 ttcgtcctcg attccaaagt cgtcgcctta tggttcttca tcgttgcctt tttgcccaat
      841 acctgtattg gtgcggcctg tggtgctgtt ttatacaaag tcctgccgat tcggaaattt
      901 gccgaagaat tgagaaaata ataatatcaa cttgtttatt gacttataag gtgctgtata
      961 ttaaactata aattggtact gagttttata gtttgagttt caaaagtatc accttgtatt
     1021 tttttgagta ggacgtgttt acatgacaga aaatatccgc gccaagattc tggccgaagc
     1081 ccttcgtgaa attaaccgcc atggttccaa tttccacatg gacgacttag cccgtaatct
     1141 gcgcatcagc aagcggactt tatacgaaaa tttttcgtcg aaacaggaaa tcatcgaaca
     1201 ggccatcgcc gattttaccg ataagatcta tgcctatcat cagcgtatcg tcagcgatcc
     1261 ggccatgact tgcgaagcca agctcatcgc ttatttcgac gtgcccagtg acaactggca
     1321 ggtcctgtcc gtgcggaaaa catcggatct cctggcaaag atgcccggta cttgtgaacg
     1381 cttgcagtcc cgctataaaa aagactggct ccttttggaa cggctcctcg atgaagctca
     1441 ggagacagga gaattcaagc cctttgataa gttcctgttc ttgcgtatcc tgcatagcgc
     1501 agccgatgac attatcgatt attttaatga tgtcaaacac gattccacct ttagtgacta
     1561 tatgaagcgc tgtatcaacg tcttgcttta tggcattaaa aatcaggaat cgaaagaaat
     1621 aggaggaaag tgaagatgat acaaaaacgc aaacttgcga cgatgagcgt taccgtactc
     1681 accatcgccg cgctcttagc cggctgtggg gccaaccaaa gtgcgactaa ggctcccgtt
     1741 gcagtcaaca cgtacaaagt cgtcgccgcg gacacgccgg tcaaggccga gtacagcggt
     1801 accgtgacgg caacagacaa ggtgcctgtc cgtcccaaga tttccggccg tgtcgtagaa
     1861 aaatacgtcc aaggcggtca ggaagtcacg gaagggcagc cgttgttccg cctcgacagc
     1921 cggacctatg acgccgccct ggcccaggcc aaggctaccc aggcccagtc cgaagccaat
     1981 ctggccaatc agcagctgaa cctgcgccgt taccagaccc tggccagcca ggacgccatc
     2041 ccgcagcaga ccgtcaccga ccaggaagcg gctacgcagc agcagcaggc tgtcgtcgaa
     2101 gccaatgcag cccaggtcga cgctgctcag gataacgtcg acgataccat tgtctatgct
     2161 ccgttcagcg gcaaattgaa cgtcgatgac gtacctatcg gtacctttgc gacggccggt
     2221 tcgacggcac tggtcaccat ttcttcgacc aatccggtct atgtcgaatt ctccatcagt
     2281 gaagccgaat acctgcagat gaccaagcag gctgcggaca acgccggcag ctggggcgac
     2341 catctcctgc tccgcttgag cgacggcacc atgtatcctt atgaaggcca tgtcacccag
     2401 gtcaaccacg gcatggatgg caactctggc tcgatcatcg tcaaggccgt cttcgacaat
     2461 ccggataatc tcctcatccc aggcctctat gcgacggtcg tttccgatac ccagatccag
     2521 cgcaatgcgc tgtccgtacc gcagcgcgcc gtccagcaga cgctgggtaa atattatgta
     2581 tccgtcatcg acggcaatgg cgaagcccag aaccgggaag tcacgccggg ccagaaagtc
     2641 ggcaaattct ggatcatcaa cgatggtctt caggatggcg atacgattat cgtcgacggc
     2701 tatcagaagg ccaaaggcgc tgccttggac cagcacctgc tgaccaaggc cgatatcgac
     2761 aacgacagct ccgatacgtc gtcggacaca tcatcgtcaa atagttagga ggcgggatcg
     2821 tgatatctaa gttctttatc gaccggccta tcttcgctat cgtcatttcg ctgatcattt
     2881 ccatcttcgg cctgttgtcg atggcgactc tgccggttgc caagtatccg caggtcacgc
     2941 cgccgcaggt ccgcgtctcg gctgcttata tcggcgcgaa tgccgaagtc gtcggcacga
     3001 ccgtggccag cgtcatcgaa cgccagcttg tcggtgtcga taacctggtc aatatggaaa
     3061 gctcgacgaa cgataacggg tcttactcca tgaccgtcca gttcaagtcc ggcagcgatg
     3121 gtgatatgga cacggtcaac actcagaacc gcgtcagcca ggtccagtcg accttgccgt
     3181 cagaagttac ccagaccggt gttacagtcc agaagtcgac gagttccatg gctatggtct
     3241 tcgccctggt ttcgcccaat ggctcttatg atgcgacgtt catgaagaac tatgctaccc
     3301 aatacttcat ggatgaactg aaatccgtcc cgggcatcgg cgacgtccag gaattcgggt
     3361 ctgactatgc catgcgcatc tggctcaacc cgatgaagat gaatatcttg aagattacac
     3421 cgaccgatgt catctcggct atcagcactc agaataaaca ggccgctgtc ggcaccatcg
     3481 gctcccagcc gtcgccgaac aaccagacct accagtatac cctgcgtacc gacggccgct
     3541 tgcagacggc ggaacagttc aagaacgtca tcgtccgcac caatcccgac ggtaccatgg
     3601 tccgcgtcgg cgacattgcc gatgtcagcc tgggatccaa ggattattcc gttgccagta
     3661 acctcaacgg cggcaaggct gccggtttca tgatcagcct gacagcagac gccaatgcca
     3721 tgcagtccgt cagcggcgcc cgcaaagtct tggaaaaggc gaaacagtcg ttcccatctg
     3781 acttggatta taagattgtc tacgatagca cgaccttcgt tacggcatcg atccacgaag
     3841 ttattgaaac cttcatcgaa gccctgctgc tggtcgccct catcgtttac atcttccttc
     3901 agagcggccg gtcgacgctg atcccgctca tcgccgtccc tgtatccctt ctgggtacct
     3961 ttgcctgctt caaggtcctc gatttctcca tcaacaccct gacgctgttc gccatggtcc
     4021 tggccatcgg cctcctcgtc gacgacgcca tcgtcgtcat cgaagccgtc gaatacgaaa
     4081 tcaagtacaa cgcccgaagt ccgcgggagg cgacgatcat cgccatgcag aacgtacaga
     4141 acccggttat cggtgttgcc tgcgtcctgg ccgccgtctt catccccgtc ggcttcatca
     4201 gcggcatgag cggtatcctc taccgccagt tcgccctgac cattgccgtg tcggtcctca
     4261 tttcggcctt cgtcgctttg accctgacac cggccatgtg tgcgtcgatt ttgaatgtcc
     4321 acaaacccaa tgacaacccc aagggcattt acaagttctt ccagaaattc aatgatgcct
     4381 tcgataggct catcgcctgg tatggcctgc gcctggctca cttgcagctc cacctcaaat
     4441 ggtgcgtggc cttcctggtc atcatcagcg ccctcacggc aggcgtcttt aaaatcatgc
     4501 cgacaggctt tgtcccgtcg gaagataatg ccttcgtcat gatgaacgtc accttgcccg
     4561 aaggcatttc ccagacggaa acgaaacaag tcagtgaaca aatcagcaaa tggctggaac
     4621 agcagccggg cgtgaccagt gtcatgaatg ttgtcggttt cagtatcctg gccaatgggc
     4681 cgaaatcgaa tggggctacg tccttcatcg gcatgagtga ctgggatgaa cgtaaagata
     4741 aatccctgtc cgtcgacgcc ctggtcggca agatcatggg catgggcaat cagattcctc
     4801 aggctacggt catcgccatg aacccgccgc cgatcgacgg catgggcacg tcgtcgggct
     4861 ttacgatgca gctggaaaac cgcggcagcc atacgacggc cgaattgtca gcgatggcac
     4921 agaaattcat cgccgctgcc cggaagcggc cggaaatcgg gtcggtctac acggcctttg
     4981 ctgccgatac gccgggctat gagctcgaca tcgaccgcga taaagttgcc aaagaaggcg
     5041 tttccctgag cgacctctat tcgacgctcc agtccttcta tggttcctat caggtcaacg
     5101 atttcaccat gttcggtcgt aattttaagg tcgtcgttca ggccgctccg gaattccgtg
     5161 aaaccatcga tgccaataac cacatctacg tccgcaacag tgccaaccag ctcgtttcgg
     5221 tggccaactt tgtccgaccg aaagccatcg gttcagcgtc gatcctcaac cgtttcaacg
     5281 actatccggc catcaagatt caaggggccc cggctaatgg ctacagctcc ggtgatgccc
     5341 ttaaagcgct ggaagaagtc gctaaagaca cgctcagtga aggctatgcc tatgaatgga
     5401 gcggcatgag ccgtgaagaa gtcgaagccg gcaacaagac ggtctacgtc tttgccctgg
     5461 ctatcctctt cgtcttcctc gtcctggccg ccttgtatga aagctggaaa gtaccctttg
     5521 ctgttatgtt cagtctgccg gcaggcgtct tcggggcttc ggcctttgcc tacctcctga
     5581 accagcagaa taacctgtac ttccagattg gcctgttggc ggtcatcggt ctggccgcca
     5641 agaacgccat cttgattatc gaatacgcta aagtccgtgt cgatgaacgc ggtatggatc
     5701 ccgtatcggc tgctatcgaa gccgccaaga tccgtctccg cccgatcatc atgacgtccc
     5761 tggccttcgt cgtcggttcc atcccactgg ccatggccag cggcgccggc gccgcatcgc
     5821 gagtcgcgat gggcatcacc gtcgtctttg gtacgtccat tgcgaccatc ttcggcgtct
     5881 tcctcatccc catgatgttc atcatcgtag aaacattcgg acacaaacga gtccgtccta
     5941 aacaaacagt cggaagactg aaagatatgt agtatgaaaa aaggcttgtc gcatgtgcga
     6001 caagcctttt tgagtattca gtttgtagtt tttagtgaag gtttttaaat ttaaagccat
     6061 cttctacaaa ctacaaactc cgtactacaa actataaagc caaagggacc tcacatgatg
     6121 gcagaagcct tcgtgcgata gtcccttttt ttagtggtta gcggatggtt aaaaatttga
     6181 aaccatcttc tacaaactct gtactacaaa ctatgttgaa agaacaacct ctcagtcagc
     6241 tgcgctgaca gctcccctag caggggagcc gggccacgag ccactagcca cgagccacta
     6301 acccctaatc tctcgccagc agccgttctt tcatttccag gtagcgtgtc gtgtagtacg
     6361 gtgccagctg ggcttcgcgc aggtcgctgc cgaaggggcg gcggctgatg gagaggatag
     6421 gcgggctctg gatgcgcttg gcgacggcga agccggcgat gaggcgccac caccgttcca
     6481 ggtcgtcgat gaaggctttt gccgtcggga agagctgatg gacatcgacg ctgcagccga
     6541 tgttcttttc cagggtgccg tcgatgtacc attcgaggat ggtttccggc gtggcccgct
     6601 gccagcgttc gacgaaggcg gcgaagaggt agtcgtgata ggggtagatg atcgggtcgc
     6661 cctggccctt ggtgatatcc tgcttgtccg acagttcggc gctggggacg atgtcgatgc
     6721 tgccctgggg cacgacttcg cgctggaaga cctggtcatt gaggtagcgg gccaggtcgt
     6781 agacctgatg cttccacagg tccgccgtag ccgccaggta gccggcgctg tcgccgtaga
     6841 gggtggcgta gccgacggac agttcggcct tgttggcatt gcacgtgaag acgccgccaa
     6901 aggctgccgc cgctgcggcc aggatacggc tcgaccggtc gcgggcctgg atgttttctt
     6961 caatgaaact ggtcagttcc agatgaccct tgtcctggcc tttctggctg agcccggctc
     7021 cttcgaactg gtgccgcgtc agttccaggc tgtcctggac ggggacgacc gtgtacaggc
     7081 agccgatgtt gtcggccagt tctttggcca ggctcttggt catgtccgag ttgtagcagc
     7141 tgggcatgtt gaccagcaga atgttttccg gcggcagtac cgtcgcatag agggcggcgt
     7201 tgacggccga gtcgatgccg ccggagacgc cgaggacgac tttatggacg ccgatggcat
     7261 ccatgaagga tttcaggccg tagtgcaggc tggcgtagat gtcttcgatg ggagactttt
     7321 ccggctgcaa gtgcgaaggc gagacgaaac gctgggcctt ttcatcgaag tcgacgatga
     7381 ccatttcttc cgtaaaaggc gtacattcga cgtgctgcga accgtctttc tggtacgtgc
     7441 tgctggcgcc gtcgaaggtg tagatgttct tgccgttgtt ctggataccc gtgcagttga
     7501 cgtagatggc cgggcagtgg accttgtcga tggcctggcc gaagaggcgg tgacgccgct
     7561 gggtcttgcc cagggtgaag ggcgagttgg aaatgttgat gaacatgtcg atgtcgtaca
     7621 ttttccctaa atagctcatg ggcttgaaag gatagttttc atcccagctg tcttcgcaga
     7681 tgagcggccc catgcggaac ttgcggccgt cgtcgaaggg aatctggacg ggcgacagga
     7741 agtcttccgg gaaggcatgg cgttcctggg cgacttcgat gagcgacgtg aagtagcgga
     7801 tgtcgctgaa ttcgcggtag ttgggcagga gcgtcttgat gtagaagggg tagggcgagc
     7861 gggccggcga aatccagcgg ccgttgtaag cgatgaacat ggtgttatac ttccgcgtcc
     7921 ggccgtcgag gttgacccgg tccgtctctt tggcgacgtt gccgaagatg atggccatgc
     7981 cgtcggacag ggccttgatt tcttcaccga agcggacgca gtcgtcgatg tagtcgggct
     8041 ggtcccaaat atcaccgatg agatagcccg gaatagcgag ctccgggaag acgatgaggt
     8101 cgcagccggc tgctttggcc ttggcgatac attctttcat gacggccgtg ttctgccgtg
     8161 gattgccagg gtggacattt gtctgggcaa gtgctatttt aaacaaggaa aagcctcctt
     8221 ttcgtagggg aaaaactatg tactatctca tcttatcata aaaaggggca ggcgaaaagg
     8281 ctgtccccgg ccaggggcgg aaaagacatg acagaacgcc gtccttgcgg tatacttaat
     8341 acatataaaa atttaaagag aggctttcat gaaaacactg cttaccatct tgaattccaa
     8401 attcatccaa tcgtccctgg ccctgcgctg cctgtcgacg gcgtgtgacc accagggcgt
     8461 cgccgtgtcc gtagccgaat atacgatcaa tcagaacagc tatgacatcc tgcgccacat
     8521 cgcctcgttc gaggccgacg tcatcggctt ttcctgctat atctggaata tcgaaatgac
     8581 ctgtcatctc atcgacctgg tgaaacaggt ctttccccag accaaggtct tcgtcggcgg
     8641 cccggaagta tcctatacgg cccggcagat actggaagat tatgagcata ttgattacgt
     8701 cctccaaggt gaaggggaag agatggtacc ggctttcctg aaatccctgg cagccggccg
     8761 cgatgggaca gaagtccccg gcgtcctcgg ccgcgtcagc ggcgtcatcc agggcgacga
     8821 gaccttccag gaagtagaag acctggacag cctgcctttt ccttacgagc atcaggactt
     8881 ccgcgaactg gaccaccgca tcatgtatta tgaaagctcc cggggctgtc ctttccactg
     8941 ccagtactgt ctgtcgggca tgaacgaccg cgtccgcttc cgctcggtcc cgctggtcct
     9001 gaaggaactg caggctttcg tcgatgccgg cgtccatcag gtcaaattcg tcgaccgcac
     9061 tttcaactgc agcccggccc atcaccggcc gctcatagcg tacatgattg ccgtcgataa
     9121 gcccatcaat ttccacctgg aaatcgagcc cggcgtcctt accgatgacg acatcgacct
     9181 cctggcccag gcaccgaaag gacggatcca gctggaaatg ggcatccaga cgacgtatga
     9241 gccgacgctc caggccatcc accgtcataa tgactggccg cgcattacgc atatcatgga
     9301 acgccttgtc gccttgggga cgatgcatct ccatttagac ctcatcgtcg gcctgcctta
     9361 tgaagactac ggccatctgc gccagtcctt caacgatatc tatgccctgc acccccataa
     9421 attgcagatc ggtttcttga agctcctcaa gggctccggc atccgccgcg actatcctga
     9481 cgattaccgg tacgacccgc aggggcccta tgaagtcctg gcgacgacgt ggctgcctta
     9541 tgaaaaagtc gcctatatga aggtcctgga agacgtcttc gagcggactt ataattccgg
     9601 gaaattcact tatttcttca actggctcgg ccgccgctat gctccggatt atatgaaact
     9661 ctacgaggcc ttgacagacg agtggctcgt cctgcataat gaccaaaagg ccctgtccga
     9721 tgagtccttg tgtcatttcc tctgccactg catcgaagat atcctgcccc tgacagaaga
     9781 ccagcagcgc ttggccaaag acctgctggc cctggatatg ctgacctttt tccgcttccg
     9841 cctccatccc gatttcctcg gctggtgcga agcgccgcgc agccagaccg acgccatctt
     9901 ccgcgacgaa gcctggctcc gccaatttgc cagggactat catttcacca actggcgcga
     9961 catcaagatg cgctatcgca tctggcctgc cagcccggcc ctgaaagggg aactgcgccg
    10021 ctacgggccc gtcccggaag gcgcggccta tatgctggct gaaaaaaaga aagaccgggc
    10081 cgaatggtgc gccatcccgg tcaaaggaga accatcgtga acgaacgtta ccgcgtctac
    10141 tcgacgtatt taaaagaaac atatggcgaa aaagtctata aactgcctat cagcatcccc
    10201 gatacctgtc ccaaccgcga cgggaccctg ggcgtccggg gctgtgcctt ctgcggttcc
    10261 atcggcgccg gctacgagaa cctgccggcg acggtgacca ttggccagca gatcgagcag
    10321 aacatggctc acatcaagcc caagtacaag gccaataaat tcatcgccta tttccagaat
    10381 ttcaccaata cctacctgcc gccagagcgt ttccgggatt acctcgccgc cgcctgccag
    10441 cccgatatcg tggccctggc cgtagcgacc cggccggact gtctcaacga gacctatctc
    10501 gatatcatgg ctgacgtcaa agaaaggtac ggcgtcgata tcctcgtcga actgggcctg
    10561 cagacggtca actataagac cttggccaag atcaaccgcg gccataccgt tgccgaatac
    10621 atcgacgcca tggtcatgct ccgggagtat cctttccgga cctgtacgca tgtcatcctc
    10681 gacctgccct gggacaccat ggaagacacc gtcgagacag ccaagaccat tgccgccctc
    10741 ggctctgacg aagtcaagct ccacgccctg tacatcatta agaacacggc catggccgaa
    10801 tggtatgccc agggaaagat caagctctgg acctgcgaag agtacgtccg ccgcgtcgtg
    10861 gccttcctcg aatacagccg gcccgatacg gtcttccagc gtctcatcgg ccgggcgccg
    10921 aaagaagcga cggacttcgc caactggggc atgggatggt ggcgcatccg cgatatgatc
    10981 gatgaagaac tggaaaaacg cggcacctgc cagggagccc gctgtgacta cctgcacggc
    11041 aaggccgtcc gtaaattcat gaaataaagg agtatctcac tttggtagaa aagaaactgc
    11101 aattttccga tttgcgtgaa gcccgggccg tcctgggcat gaaagacgaa ttcctcaaga
    11161 cgatgcagcg cgaattcccg gactgccgca ttgccgtccg cggcgatacg gccatgatcg
    11221 tcggcagcga agaggatgtg gcctatgtga ccgaagtctt ccgcgtcctg cgctacttgt
    11281 tccgggaaaa tacctatctg acgacgcagc acgtccgcta ttcggccaga ctcatcaaag
    11341 aaggccaatc ggaggccctg cacaacctct atgaagatac ggtcggcatt tctgcccggg
    11401 gccgtctcat caagcccaag acgctgggcc agaaacgcta cgtcgattcc atccgcaaga
    11461 acctggtcac cttcggcatc ggtccggccg ggacgggcaa gacctacctg gccgtagccc
    11521 tggcggcttt ttatctcaag aaccgcgaag tcgaacggat catcctgacc cggccggctg
    11581 tcgaagccgg ggaaaaactg ggcttcctgc ccggtgacct gcaggaaaag atcgacccgt
    11641 acctgcgccc gctctacgac gccctggccg acatgttcgg ctatgaccag ttccagaaga
    11701 tgatcgaccg caacatcatc gaagtggcgc cactggccta catgcgcggc cggaccctgg
    11761 aagaatcgtt catcatcctc gacgaagccc agaacacgac gcgggaacag atgaagatgt
    11821 tcctcacccg tatgggcaac cactcgcggg tcgtcgtcaa cggcgatgcg acccagatcg
    11881 acctcgtcga ccggcgcatg tcgggcctga cggaagccga aaagatactc aaagacgtcc
    11941 gcggcgtcgg catggtctac ttcagcgatg aagacgtcgt ccgtcacgat atggtaggcc
    12001 gcatcatcag ggcctacgaa acttattatc atcacgatca ctagattggg ggaattgcca
    12061 tgaatatcaa catcaattac gaagatccgt cgttccagca cgatgaatat gaaaaagtca
    12121 tcgaccgcgt ctgtgaagaa gccgccctgg tctacgacct ggggccgaag gctgaaatca
    12181 gcatccttct gtgccataac gactatatcc atacattgaa caaacagtac cgcaacatcg
    12241 accggccgac ggacgtcctg tcctttgccc tcaacgaagg ggaggaagac ggctatgacg
    12301 gtcccgatac ggccctcttg ggggatatca tcatttctct ggaaaaagtc caggaacagg
    12361 ccgacgaata cggtcattcc tttgaacggg aactggccta tctgaccatc cacggcatgc
    12421 tgcatatcct cggctatgac cacatggaac cggacgataa agcagaaatg cgcaaagaag
    12481 aagaattcat tttacagcgc ctgggctatg tccgcgaagg cgaagaatta taagggagga
    12541 attacgatat ctatggaatc atttaaatcg ggtttcgtcg ccgtcgtcgg ccgcccgaat
    12601 gttggcaagt cgacactcat caaccgcatc atcaagcata aagtctcgat cgtctccgat
    12661 aaggcacaga cgacgcgcaa ccgcatcctc tgcatccata cggacgacga atgtcagatc
    12721 gtcttcctcg acacgccggg catccacaag ccgaaacata agctggggga attcatggat
    12781 gaagcggcct atcagtccct gcgcgacatc gacgccgtcc tgttcctcat ctcgggcaat
    12841 gaaaagaagg gccctggcga cctcttcgtc ctggataagc tgaaagacgt cggcgttccg
    12901 gtcttcttga tcatcaacaa agtcgacctc atgacgaaag aagagatact gcacaagatt
    12961 acggaatatg ccgaactcta tcctttcgcc caggtcatcc ccatttccgc cctgaaaggg
    13021 gacaacgtcg atgccgtcgt cgacgaactg cgcaagatcc tgccggaagg gcccaagtat
    13081 ttccctgacg acatgatcac ggaccagccg gaacgccttt tggtcgctga aatcgtcagg
    13141 gaaaagctct tccgctgtac ccgcgatgaa gtgccccatg ccatcgccgt ctatgtcgaa
    13201 gaaatgaagg accgcggtca caataaggtc tatatccggg tcacggtcta tgtcgaacgg
    13261 gattcgcaga agcgcatcgt catcggcaag aacggcacgg tcctcaaaga agtaggcaac
    13321 ctggcccgcc aggaaatcga aaacctcctc ggttcttcgg tttacctcga catctgggtc
    13381 aaagtcaagc gggactggcg caataagtcg ggcgccctca gcgaattggg ctataagaac
    13441 gagtaggggg ctgcatgatg gaagtacaat caaccgtcca atgtctgctc gtctttctca
    13501 tctgtttggc cgtcagcgcc atctgcgtca ccggtaaatt cgcctttgcc cagctgcgcc
    13561 gggaatacct ggaagaactc gtccgcgacg gcgatacgtc gctgaaaccg ctcttgtccc
    13621 tctacgacaa gcccatcgtc tttctgggga cggcccagct cggcatggcc ttgtccggcc
    13681 tcatcgccgg cgctgccgga ttctgcggcc tctatggcct ctacggcctg ctggctccgt
    13741 atatcggcca tacgtggctc atctggctca tcgaaatctg cgccggtatc gtcctctgct
    13801 tcatcctctg ggtctttggc gagctcatcc ccaagtccat cggcctccag cgcccggaaa
    13861 agagcctcaa ggccctgcgc cgcttcatcc agccctggag ccggcccttc atgcccttca
    13921 tcctcttcgg gaactggctg ggccgggcca tcctggcaga ccgcaacctc gatgtgacca
    13981 atgaaatcga tatggaccat tcggaagatg aaatccgcat gctggttacg gccagccaca
    14041 aggaagggaa aatcgaccag gtcgaaagcg aactcatcgg caacgtcttc gatttcgccg
    14101 accgcctggc caaggaaatc atggtgcccc gccaggacat cgtctgcctc tatacggaag
    14161 atacgatgaa ccagcatttg cagaccatcc gccagtcgcg gcatacgcgc tatcccctct
    14221 gtgaagacga taaggaccac gtcctgggcc tggtccacat caaggacatc atggacctct
    14281 atatccacaa gcgcagcaac ttgaacctca tcaagcggcc gatcctcatg gtcccggaaa
    14341 tcatgccggc atcgaagctc ctccagctca tgcgcatgcg ccggacctac ctggccatgg
    14401 tcgttgatga atacggcagc accgtcggcc tcatcggcct ggaagacatc ctggaagaac
    14461 tggtcggcac gatccagaac gaacacaccc aggaaaaaga agaaatccag cccctgcctg
    14521 acggcgccta tgaatttgcc gggacagtcc ttctcgacga cgtcgaagag ctgctgcaca
    14581 tccccgtcga agacgatgtc gataccgata ccatcggcgg ctacgtcttc aatgccctgg
    14641 gccatacgcc gaaagtccac gacgaagtca ctatcggcgc ctactgcttt acagtcctgg
    14701 aaatgcagcg cttccgcatc gtccgcctga aagccgtacc cctgccggga gcggctgccc
    14761 ctggggaaga cagcggggat gaagaagata agactcatga atcgtagcgg ctactatggc
    14821 cgcaaaccgt cgcggtccta tgaaggcatc atcctcaccg tccgcaagac cagtgccaaa
    14881 cagctcgtcg tcacggtcct gacccggcag gccggcatcc tccacgtcct ggcccgccgc
    14941 agcactcagg gcaagatggg ctacggcgcc ttgacgcctc tggccgagat gacctttgac
    15001 gtctttgaaa aagacggcgt ctacacgctg acggaatacg actgccgcag caaccagcgc
    15061 ctccgcgagc tgacttggga cggctatatc tattcgcaga tcttcgtcga aatggtcctc
    15121 ttcatggccg gcggcggagc cgatgaagtc ctgtacgatc tggtccgcct ctatagccgg
    15181 gccatcgaac tcaaggacat ccgcatcgtc accatcatcg ccggctggca gctcatggcc
    15241 ctgacaggct tcctgccgga catcgacacg gcccgcgtct tctgtcaggg ccagggctac
    15301 tgccggcagc ccgtctatta catcgacgat gaaaggccgg aaaacatgaa agaactgcct
    15361 ctgacgccgt ctgtacgcca gacctggaag accctggtcc actatccctg gggaacccaa
    15421 gaaaccgtga acctgcgcat ggccgacctg aaattcctgg aagccctgct ctacaactac
    15481 gtcaaccagt gcagcgaacg ccagatgaag acaccggaat tattagtggc tcgtggctag
    15541 tggttcgtgg ctcgcggatc gcggatcgta ggggcggccc acctggctct cttgatagga
    15601 gagctgtcag caaagctgac tgagaggttg ctcttttaaa tagttcgtgt ttcgtagggg
    15661 tcgccacgtg ggcgacccgt tagggatttg gatgattctt ctagcagact ggtcgggaaa
    15721 tcgtacatat ttcagagcgg gcgccccacg tggacgcccc tacaaaaaag aggctgtcgc
    15781 gcatgcggca gcctcttttt gccccctcac cgcttatgca gcgggtcgta tttccaatga
    15841 tgggcgtatt cttttagact ttcggcgacg gcgtattggg ccgtatcggt atagaccatt
    15901 tgcgtatcta cggactcttc ccagatgggg acaggccagt cgcggggcgg ttcgatgtaa
    15961 tagagcagga attccctggt cttttcgatg aaccattccg gcaggcgggg gtggaatttc
    16021 ttgtagaaga tgaacaagtg gatgtgattg ggcatgaccg tgaactcttt gatgatggca
    16081 atctggttgt tccgctgcat ggccttgagg gcccgcgtcg ccgccatgcc ccaggcggtc
    16141 ggccgccaga tgtgctgtcc ctgatagcgc aggactttcc ccagtgtttc ttccttttga
    16201 tacgtacaaa aggtgatgag ctggcaccgg ggaacggtag agaagggttc ccgccatgca
    16261 ggaagggaga aaaacggttt gtgatacatg ggcaacacct ccgaaaaaaa acaaaacgtt
    16321 taaaacaaga tgcgtgttac gcctattgta ccctggaaat atgaaaggga cattaaaaaa
    16381 tcaggggcct tgattaaaaa atgattaaaa cttgtttttg tgccctatca tgctataata
    16441 aaactatttc actccttgtt attagagaaa ggacgattcc atggttcgca gtatgacttc
    16501 gggcaatccc atccgcctca tcctggcttt tacggtgccc cttttaatcg gcaatgtttt
    16561 ccagcaattt tataatatcg ccgacatcat catcgtcggc cgcactatcg gcgtcaatgc
    16621 cctggccgct gtcggcgccg tggcacccgt cttcatggcc gtcttcggcc tgacgatcgg
    16681 tttgtcttcc ggtttttccg tcatcacggg tcagcgcttc ggtgccgaag acatggacgg
    16741 cgtgcgccgg tctgtggcga cgtctgccat gctggccctg gtcatcaccc tgatgctgac
    16801 ggcctgcgtg agcctggcca tgccgctcat catgcggctc atgaacattt cagatgtcct
    16861 ctacgacgac gcctatcatt acatgctcat cgtcgtcctc ggcctggtag ccatgatgag
    16921 ctataatctc ctgtcctgca tctgccgggc cctcggcgac agccggacgc cactttattt
    16981 cctcatcgtg tcgtctctct tgaatatcgc cctggccctg ttgttcatcg tcgcctttgg
    17041 ctggggcgtc tccggctcgg ccatcgccct ggtcatcgcc caggccgtat cggccgtgct
    17101 ctgcctgtgt ttcatgcgcc gccgctttcc catgctgcgc ctgacccgag ccgactggaa
    17161 ctttgactgg ccctttgcct ggcagcattt gcgcctgggc ctgcccatgg ccgtccagtt
    17221 ccttatcatt tccatgggca tcctggtcct gcagtcggtc tgcaatacct ttggccctga
    17281 aacgattgcc ggcttcgtat cggcgacgaa aatcgaacag ctggccttgc agcccatgat
    17341 ttccttcggc atcgccatgg ctgtctattc ggcacagaac tacggcgcca gccagtatgg
    17401 ccgcatccgc cagggcgtcc gtcagtgctc cctcgtgtcc ctggtattct gcctggccgc
    17461 agccgtggcc atgtacagct atggccggga actcgtcagc gtcttcacga cggaccacga
    17521 cgaagtcctc atggaacagg ctttcctcta tctgaagatg tccgtcccgt tctatctctt
    17581 cctgggccag atcttcgtct accgcaacgc attgcagggc atgggcattt ccagcgtgcc
    17641 cctcatcagc agcatcctgg aactgggcgg ccgcagcgta tcggccctgg ttctggccgc
    17701 catgtggggc tacttcggca tctgctgtgc cagccctatt tgctgggtcc tggcctgcct
    17761 atttacaggt ggttcctatt tctgggtcat gcacaccatg aaggccaacg gctgttaaaa
    17821 agggaagggg acagtctatg aatacagaga aagaaaacaa tatgacttcg ttaggagtat
    17881 gaagcatttt tttagcaagc tgattgggaa aacgtacata ttacaaagcg ggcgccccgc
    17941 gtggcgcctc tacagaaaaa gcgccgtccg cgaggacagc gctttttttc tattcaaact
    18001 gattgactaa ttctatgagt ctttctttat gctggggata ccattcgttg taggactggt
    18061 tggcatcgaa aagggtctgg ttgagggcca ggaagaagtc gggcaggtgc tcgatggtga
    18121 tgaccccgat ttgttcgccc atgacgcctt taccgatggc gtagttgcct ccggcgtaga
    18181 tgttgaaggc cggttccatt ttgtcgccga tttttttgct ggcgccgcgc aggcccagag
    18241 gagccagctg ctggaccgtg cagttcgacg gacagccaga gaagtggcat ttcggcagga
    18301 agtggctgtc gatgccgcgg tctttgaaga gctgggccag tttgacgaag gtgccgtgag
    18361 aatcgcgcag gccctgctgg cagaccgtag cgccgatgca gctgacggaa tgttcaaagg
    18421 ccgtcgtcgc cgtgtcgtcc gccgtgaggg cagcgacttt acgggcttcg tctgccgtga
    18481 ggttgatgat gtaagccgtt tcgtcggcat tgaggcggat ttctgcgcct tccatggtct
    18541 ggagggcctt gagcaggtcg tggaagacgg agagtttcgg cgtgccgcct aaaggcttgt
    18601 attcgacgta atagaggccg gcctgtttct gtttgccaat gcggccgtct tcgaggacgc
    18661 tgtcatcggc gccgctctta gctaacggcg tttcttcttc gatggtcagg cggtaatcgc
    18721 cttcggcctt ggctttggcg acggcatcgc ggacggcctg gcggaaggca tcgtcgccga
    18781 gcttggtgag gatgaaacgc gaacggttct ggccgcggtg ttcatagtcg ccgtatttca
    18841 tgtagacgtc ccggccatag cccaggatgt aataggaaat atcctgcgga tcgacgcctt
    18901 cgtcgatgag gatgccgaag cgcgagccgt tgagtccgaa gccgccgccg gcatagacgt
    18961 cgaatttgtg gtccggccgg gccacgaagc ccaggtcctt gaaggtcgca tggccttcgt
    19021 tgtccaggcc gctggagaag ctgattttat atttacgcgg caagttgaat ttgggaatgc
    19081 agttgatgac gaagttggct gctgccttga tgtacggcgt catgtcgaag tattcgcccg
    19141 gagcgacgcc atggagcggg ctggccgtga tgttacgcgg attgtcgctg ccggcgccga
    19201 cgcagtagac gccgtggtcg aaacattcct gatagagggc gaggacttct ttttcgccca
    19261 aatgatggag ctgcagggcc tcacctgtcg tgaagtggac gaggtcgatg tcgtagcggt
    19321 cgaggacgtc cttcaggaaa gcgatgtgtg ccggttcgat ggcaccgccg gaaaagcgca
    19381 ggcgcagcat ggtgctatgg gccccccgtt cgccatagca gccgaacttg cccgacgtcc
    19441 ccttatattt ggccggcggc agttccttag cgtaaaaagc ctgcgtcgct tcgacgaact
    19501 gcgggaagcg gcggctgatt tcttcctttg tttcttttgt gatttgagtc atttcataaa
    19561 caccccttat ttaaagaggg caaaagattc accagaaaat ttgctttttg ttatattttt
    19621 gtttaacctt agtatgtata ctagaagaaa atttagagaa accaggtgag gactatgcaa
    19681 ctccgggact ggcggctgcg caagcgcctc cttttttcca actttatcat gattttcatt
    19741 cccgtcctgt ttacggtcgt cgtcagcatc gccatctttt tggccctgca attcggcaac
    19801 atcaaccggg ccagtatcat ttcctttatc tggcctgaga gcggtcagaa catgtccatc
    19861 cagttcgagc tgagccgcct ccgtgtccgg gccgatcagt ataatggcaa catggaccgt
    19921 ctcctccagg ccgccgatca tttggaggac cagggcctca atgtatccat ttggcgcgat
    19981 aaggaagacc tctacgatac ggatggcatc gacaaggtgt acctgcgcca gcaagtcatg
    20041 gccggtcatc gtcagggcca tgccgacttt acctggactg gcgaaggcct gcgcttttac
    20101 tacgtctcgc ctcatacgga catccatttg gccgtcgccg gtgccgtgcc gagttattcc
    20161 gaaaacgagt acatcgactt gtcatcgaag gacatcttga aggtcgcctt ttacgtcctg
    20221 gctattctga ctatcttgct gaccatcctc gtcggcctga aactgtcgcg ctggatggcc
    20281 ggccagatcg tcggtcccgt cgaaaggctg cgcaatatgg ccgatgccat cagccagggc
    20341 gatttggacc atcccgtccc gattttgtcg cgtgacgaaa tcggcgatac ctgccaggct
    20401 tttgaaaaga tgcgcctcca gctgcgggct gcccgggata cgcgggaaaa atacgacaag
    20461 aaccgcaagg aactcctggc cggcatttcc catgatttgt cgacgccttt gacgaaaatc
    20521 gaaggctatg ccagcggcat cctcgacggc atcgccaata cgccggaaaa agaacggcac
    20581 tacctgggca tgatcgtcga tacgtcgcgc cacatggccg gcctggtccg gacgctcttc
    20641 ctcttttcca agctcgacct gggccaggta cccttccatt gggaagacgt cgacgtggcg
    20701 gcctatttaa aggattatat tggcgaacag acggaccatt tccaccagca agggctggac
    20761 gtgaccttcc agtcctccct ggacaaggcc gtcatttcta tggaccgtca gcagttccag
    20821 cgggtcattg aaaatatcct gggcaacagc ttgaaatata aagaccaggc cgtcggccat
    20881 ctggccatta tcctgaccga tggcggggaa ggggcatacc tcctttcttt tgccgatgac
    20941 ggctgcggcg tcgaagagaa ggaactgccg aagctctttg aaagtttcta ccgcaccgac
    21001 aaggcccgga ccaatgtggc caagggaagc ggcttgggcc tggccgtggt ccagcagatt
    21061 gtccatgcca tgaagggaaa aatctgggcc caggcgacag tgcctaaggg attgaccatt
    21121 tgcatttcgt taccgaaagg ggaatcacat acatgaaacg tatcttgatt attgaagacg
    21181 accagggcat tgccgaattg gaaagggact acttggaagc caatggcttc agtgccgaca
    21241 tcgcggtcga tggcaagaca ggggaaaaga aagccctgga agacgcctat gacctcatcc
    21301 tgctggacat catgctgccc ggccgcgatg gcttccagat ttgccggact atccgcgaaa
    21361 ccaaggacat cccgatcctc atggtatcgg cccggcagga agacatcgac aagatccgcg
    21421 gcctcggcct gggcgccgat gactacatcg tcaagccctt cagtcccaat gagctcgtag
    21481 cccgcgtcaa aggtcacctg gcccggtatg aacagctcac gtcccgcgaa gtccatcgcg
    21541 acgtcctgca ctacggcgac ttggaaatcg atgagagcgg ccaccgcgtc ttcgtcaagg
    21601 ggaaagaagt gaccctgccc aataaggaat tcgagctcct cttgttcttt gccaagaatc
    21661 cgggcatcgt cttcagcaag gagaccctct tcgaccgcgt ctggggcagc gaagccctgg
    21721 gggaaacggc gaccgtgtcg gtccacgtca accgcatccg cgaaaaaatc gaagaggata
    21781 cggccaaccc ccgctacatc gaaacggtct ggggcgccgg ctaccgcttc cggggttagc
    21841 ctgacggaat tttttacggt cctttctcgt tttgttatcg ctctgtttat cttttattta
    21901 acttcccttc ctatactgat accatcaaaa cggtaacgaa taggaagggg agtttttatt
    21961 atgtatgaat ttcgtgcaaa acaggaagac gtggccctgt cggtatggaa gcgcctgtgg
    22021 cagaaaatca tggcttcgcc ggctgccatc ctcctggtcc tggcagccat cctctgtttt
    22081 gtggccaacc acctgctggt cgtcacggac cctgtcgaat cgaactatgt tctgacggct
    22141 aaggaaatgc tcgcttctgg cgactacttc tcaccgcgca tcttcggcga ttactggtac
    22201 gataaaccga ttttcttcta ttgggaactc attgccgcct tcaagctctt cggcatcagt
    22261 aatttctcgg cccgcttttt cccggcccta ttcggtatgg ccggcatcct cctggcctat
    22321 ggcttcacgg cccgcctcta cgataagaag acgggattcc tgaccggcct catcttgctg
    22381 acgacggtag aatattttta cctctccaag gccatcatta cggatatgac cctcttcatc
    22441 ttttattcgg cctgcctcat ggccttttac atcgcctaca gcgaaggcaa gccgcgctgg
    22501 tattacctgg cctacgcctt tgccggcctg gccgtcctga ccaaagggcc tatcggcttc
    22561 ttccagcccg ggctgatcat cctgcttttc ctcgtatggc gccgggatct caaggccctg
    22621 ctgcacatca agctcttttc cggcctgatc ctgttcctgg ccatcaccct catctggtat
    22681 ggtcc
//
LOCUS       DAWABZ010000018        21473 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_693_length_21473_cov_3.177608, whole genome shotgun sequence.
ACCESSION   DAWABZ010000018 DAWABZ010000000
VERSION     DAWABZ010000018.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 21473)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 21473)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21473
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_693_length_21473_cov_3.177608"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1703..1712
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6281..6290
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13111..13120
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15293..15302
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18459..18468
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4249 a         5787 c         6416 g         4971 t
ORIGIN      
        1 gtgccagccc cggcgcacca gctcctgacg ggctgccaga ccgggcctgt cgccgtaaga
       61 agccatggcc atccaggcac agagggcttc ctgccgggcc tgttcataag cggcgcgcgg
      121 cgaatcggca aagacagaaa aagctgttgt cacgcaaaaa aggcacgtga aaacagcttt
      181 gtaacaagaa gtgaacatag gctttcccct ttccagccca tcagggctcg taggacaaaa
      241 tggaaaagac ttcccggttc ggatggtgtg attcttcaaa atagagagtc cgcttcagct
      301 cccgttccca ttcggctttg cgcttcttgt ccttgagcag ggccaggacg tcgggatggg
      361 cttcgatgag gatatcccct ttgatgtggc ggacctggac catatggcgc aggcggcgga
      421 tgatctggat ggccacggtt tccggcgaat agaggtagcc tgaaccgccg cagacgtcac
      481 acgggctgaa ctggacctgg tacaggctct ggcgggcctt cttgcgggtg atttccacca
      541 ggttcagggc cgtcatgccc aagacgcggg tcttggtgca gtcctgcgcc gtttcccggg
      601 tcaggatctg gaggatttcg tcgcgtttcg tcggctgggc catgtcgatg aaatcgatga
      661 tgatgatgcc gccaatatcg cgcaggcgca gctggcgggc gatttccacg gccgcttcct
      721 tgttgacgtg gaaaatcgtg tcctgcaggg tcgaaccgtt accggtgtac ttgctgctgt
      781 tgacgtcgat gaccgtcagg gcttccgtgt ggtcgatgac cagattgccc cctgacggca
      841 ggggcacgat gcgcgacatg aggtgggtca ggctcgcttc gatggcgtac tgttcaaaga
      901 tggacaccgt cccttcgtag aggcggacgc ggctgcgcca ggccgggtcg ggaaagacct
      961 ggcagatgcg ttcataagct tcgcggctgt cgacgacgac ttgtttgacg tcggccgtga
     1021 aatggtcgcg gatcatgcgc atgaccaggt cggcttcgcg gtataagagg gtcggcttgc
     1081 gggaccgctt ataccgctgg aggatgctgt cccacgtccc caggagatac tgcaggtcct
     1141 tgaggagctc ttcccgggaa acgcccttgg cgacggtccg gatgatgagg cccatgccgt
     1201 cgggcttgat ttccgatacg atgcggcgca ggcggttgcg ttcttcttca tcgcggattt
     1261 tcttggacac gccgatgtaa tcgacagtcg gcatgaggac ggcgtagcgg ccggccaggc
     1321 tgatattggc cgtgactttc ggccccttca tgccctgttc gtccttgatg acctggacca
     1381 ggacggactg gcccacggag aggtgcatct gctggatttc ttccttcgac gccgcccgcg
     1441 ggaacatgtc gcccatatag atgaaggcgt tcctgcggcg gccgatgttg acaaaggcag
     1501 cctgcaggga cggcaggatg ttctgaatcg tccctttata gatatggttg acaatgtgca
     1561 tgtcgtcatt gcgttcgaca gcgaaatcac gaagacgacc atcctccagg atagccatgc
     1621 gcgtttcttc tggcatgaca ttgcctacga ttgttttcat cttctcattc ctcctgtgcg
     1681 ggcaaattgg taatcgcaga cannnnnnnn nntttttcat agacccgttc tcccggcagg
     1741 ccgcagcgtt cgatcatcga cgccttgtcg aggagctgca ggtcccgcag ttcctcctgg
     1801 attggtgcca gccgtcccga gggcttcatg atgaccttgc tgccttccag ggccagcagt
     1861 tctttgcggt gtgcattgtt tccggggatg acgatgaggg cttcatcctt ttcgcacagc
     1921 ggctgctgga gcttggcggc gacggcgcag aaactgggca cgccgggcac gatttccgtg
     1981 tcgtagccgg cctcatggac gaggcggtgg acgtagatgc tggtcgcgta gaccgtcggg
     2041 cagcccaagg tgatgaggca gacgtcgcgg ccctggtcga gttcgtcctg caggcggcgc
     2101 gccgctttct catgggctcc ctggaggacc tggtcgtcgc gggtcatggg gaaatccatg
     2161 gcgatgacca ttttatcttc taaggaaacg ccgcagccgt ccagggcctt caagaggatg
     2221 ccctgggccg tgacgacgcc ggtccggctc tggggcacgg ccaggacggg acaggtcctg
     2281 atgattttca gcgccttcag ggtcagcagt tccgggtcgc ccgggccgac gccgacgcaa
     2341 tagagtgtac ctttcatgat catccctcaa ataacgaata tttctgaccg ttttccttgc
     2401 ggacgaaaat acccgtccgc cgggccagca tcatgtccgt gtggatgggc aggccgaatt
     2461 ggctgcccag gatttcccag acctgggacg gcttgatggc cccttcgccg gtctgataga
     2521 tgccgacagt cagatagaca ttgccgtcct tcacatggcc gtggacggct tcgacgacgt
     2581 gctgcttgac gtcgatggta cgcacgcggt gcttgccctt gtgggacacc ttttcataga
     2641 ggacgctggc tgcgtcattg aatttcgcca aaatctggtt gagtccgtcc tggctgaggt
     2701 cttccgtcag cgggccgcgc agggtataga cggcgtaatt ggcaatggcc atcagcttca
     2761 gtgccttggc atcgacgtat ttgccatcga ggacggcaaa gccctggggc gacatggcgt
     2821 tcatgcgcgc catgacgtcg gcgacgggca gcttttcagc cagttccatg tcgaggtatt
     2881 ccacatcggc cgagacaccg acgcccaggg ccgaggcgaa gctgagcttc atatgcggat
     2941 tgaagccttc agaataacag ataggcagtt tggcccggat gatgacgtag cgcacggccc
     3001 gggcaaaatc caggtgcgac aggaattgca gggccccatc tttcttaaca gctaaacgta
     3061 gtctttccat ggtcgtcccc ttcctttctg tcgatgatgg atacgcccag attcgggcag
     3121 acaccgcagc ccgtacaggg caggcggcgg cagtcgtgag tcagttcagc tcgagaggcc
     3181 ttctgccatt cgcgcttcaa ataggccttg gcggcgccgt tgctgagatg gtcccagggc
     3241 agggcttcgt cgaggctgcg cgtccgggcg gcatagtaat cggggtcgat gccggccagg
     3301 tcgaaggctt tcatccacat atcgtaatcg aacatttccg tccagccgtc gaacttgcag
     3361 cccagtttcc aggcttcgta gaggactttg cccatgcggc ggtcgccgcg ggcgaaggtc
     3421 gcttccaggc ggcccgtctt ggcatcgtgg taatggtagg aaatgtgttt gtcgttgatg
     3481 agacccttga tgtactgctg tttccgttcg atttcttcaa tcggcagctg gccgaaccac
     3541 tggaaggccg tgtgcgactt gggtacgaag ctcgatacgg agaccgtgac cttgccgttg
     3601 ttcttgtggg tcactttgcg gtagagattg aggaccttgt aggccaggtc ggcgataccg
     3661 gccagatctt cgtccgtttc tgtcggcaag cccatcatga aatagagctt gaccgtggac
     3721 cagccgtttt caaaggcatt ttcacaggct gccagcaaat cttcttccgt aacgcccttg
     3781 ttgatgacat cgcgcaggcg ctgggtcccg gcttcgggcg caaaggtcag gccgctcttg
     3841 cggacctgct ggaccttctt ggcgatatcg acggaaaagc tgtcgacccg cagcgacggc
     3901 aggctgacgc tgactttctg gcccttgaat tcgtccaaga gctggtcgac cagttcgggc
     3961 aggcaggaat aatcggccga gctcaggctc atgagggaaa tctcattgta gcccgaattg
     4021 gcgataatct gtttggccag gtcggccagg tgttccggcg atttttcccg gaccggccgg
     4081 tagagcatgc cggcctgaca gaagcggcag ccgcggctgc agccgcggaa gagctccagg
     4141 acggcccggt tgtggacgat gtcgatgttg ggtacgatcg gtttcgtcgg gaagaagacg
     4201 tgttccacat cggaaacgac gcatttctcg atttctgccg gcgctcccgg ttcagtgacg
     4261 gtcaggccct ggaagtcgcc ctttgcatcg tactgggctt catagaagga cggcacgtag
     4321 ttgcccggct gctgggagag ctgcttcaag aggcccttct tgccgcccgg ttttccctgc
     4381 tctttccagg cgatgatcgt atcggccagg acctgcgtcg attcttccga ttcgcccagg
     4441 aaaaagacgt cgatgtaatc ggccaggggt tcggcgttgt aggcacaggg accgccggct
     4501 gccaccaggg gcatgtcgtc gccgcggtct ttggcaaaga gcgggatgcc ggccaaatcg
     4561 agcatgttga ggatgttggt aaagctcatt tcgtactgga gcgtaaagcc gaccatatcg
     4621 aagtcgcgca caggcgtctt cgtttccagg gaaaagagcg gatagccctg gctgcgcatg
     4681 acttcttcca tatccggcca gggcgcgtag acccgttcgg ctgccacatc gggccgttca
     4741 ttgaggagcg catagaggat gcgcaggccc agatggctca tggcgacttc gtagacgtca
     4801 gggaaacaat aggcaacggt cagtttgacg gcatcgtggt ccttgacgac gctgttccat
     4861 tcgccgccga cgtagcgggc cggcttgctg actttgctca gtaaaatcgg atcgatctcc
     4921 acgggatgtg acaaattaat ttcctccttc acggcgggtt aaaaaatcat cgtctgctga
     4981 cgcatgtaaa tactcagtaa gaggcccagg cccatcatat tggtcgtcaa ggcgctgacg
     5041 ccgtagctca tgaacggcag agggatcccc gtgaccggca tgatgccgca ggtcatgccg
     5101 acgttgacca ggatctggaa agcccacatg gaaacgacgc ctgtcgccag catcatgccg
     5161 aagctgtcct tgcagtcctt ggcgatcatg aggccgcgat agacgaggat gaagtacagg
     5221 aacaagagga agacgcagcc gaggaagccc agttcttcgc cgatgacgga aaagatgaag
     5281 tccgtatggt tttccggcag gaagttcagc tggctctggg tcccctggaa gaggcctttg
     5341 ccaaagagca tgcccgaacc gatggcgatc ttcgactgga tgatatgata gccggcgcca
     5401 aaggggtcgg cattgggatt gaggaagacc gtaatacggg acttctggta atccttgagg
     5461 aaatgccagc ctacgggcgc caggaagacg ccggccagga agacgttgcg caagagggac
     5521 atcttgatgc ggccgacgaa gagcatgccg aaggtaatgg ccaggaagac gagactcgtc
     5581 cccaagtcag gctgtttcaa gaccaggagg aagggaatcc cgacgtagag gccgatgggc
     5641 aggacctggc gccatgtgtt gagctggcct acgcggtcgc tgaggatcga tgccgtgcag
     5701 atgatcatga tgagcttgga aaattccgac ggctgcagcg tgattgggcc gagctggatc
     5761 cagcgctggg cgcccagggc cgacgtaccg acgaacatga cggcgaccag catgagcagg
     5821 ttgatgatgt acaggggctt ggcgtatttc ttcagcttcg cataatcgaa gcggctgaaa
     5881 aagaggatga tcatcaagtc gacgacaaaa gccgccccct gttttgcgac gaagtcgtag
     5941 ttgatggccc ctttattgat atgcgtcgca ctgccgatga tgcacaggct gatgcagacg
     6001 atgaggaagc tcacgatgag catgacttta tctaaatttt tccattctcg tttaataaac
     6061 atttctctct cctatcgacg atagtggcgc caattccggc attcctgacg gcggcgctgc
     6121 agcgacaggc ccggtaagac ttcggcctgg gcctggtgtt tcggcaagag ggccgtcaga
     6181 gccgtttcct cgatggcctt ccccgtttcc agccagtctg ccgtcgccga cagggcctga
     6241 cagaaaggca gggaatcagc catacaggtg atccgccctt nnnnnnnnnn gccatcatca
     6301 tgcggcaaga gaccggcgac gtggccggtc tgcaaacggg ctttggctgc gtctggcgtg
     6361 atgacgccgt cagcgtaaaa attattgagg atgacgtcgt agtgaaagcg cttctgccgg
     6421 gccgcgtact gcatgacctt agccgcatcg cgcagggacg acggcgacgg ttcgacgacg
     6481 aacaggatct catcggccag ggccgtcgcc gcctgatagg cgccgtcgcg gccgggcggg
     6541 cagtcgatga gggtatagtc ataggacccg gacagggact ccaggacata ttgatacgac
     6601 gggatgtcga tccgctccca ggtccgcttc tggctggccg gcaggaaatc cagtcccggc
     6661 tcgaccggca gcaaagcctg gccgggcagg catttccccc gcgcagcctg ggcggcatcg
     6721 taatggatgg acccgtccag accgaagagc aggtcgaggt tgcgcaggcc catgtcggcg
     6781 tcggccgcca ggacgcggtg gccccggcgc tgcaggagga cagacaaggc cgccgtaatc
     6841 agggtcttgc cgacgcctcc cttaccggag gcgatggcga tgagctggga catgaaatca
     6901 ctttcctttc tgatgagata aggccgtcat caggacttat cggtcagact cagtcccatc
     6961 gcgatggctg ctgtccttcc cgtcctgcgc attcttattg gccttgttgt ccttttcttt
     7021 gccgtccttg tctttatcct tcggtgcgcc ttcggcgtat tcaccgacgt ggaagtaggc
     7081 ttccagcatg gaacggacaa tcgggccgga cgacagagac ccgaaaccgc cctgttccgt
     7141 caggacggcg atgacgatgc gcggatgttc atacggcgca taggcgacga accagccatg
     7201 gtccgtaccg ccgaagtttt ccgccgtccc tgtcttacca gcgacctgga tggggaagcc
     7261 ggcgaacagg gaaccagctg taccggattc ttcagcaacg ttacgcatcc cttcgcggac
     7321 caggtccagc gtcgatttgg atacggacag ggtgccgacc tgttccggtg caaaaatctt
     7381 atacggcgtg ccgtcgaggt tgtcgatacg gctgacgatg aacggccggt actggatgcc
     7441 gccgttagcg acttcgctga cgatcatggc tgcctggagc ggcgtaacca gctggaaccc
     7501 ctggccgata gccgagttga acgtatcacc gaggtaccat tcatcgtgga aattcttctt
     7561 cttatattcc gcactggcga ccaggcccgg tgcttcgccg cgcagggcga tgcccgtttt
     7621 cttgcccatg ccgaacatgc gggcgtattt cgccagttcg tcaatgccga cgcggcggcc
     7681 catttcgtag aagtatacgt tatcggactt agccagggct tcgacgaaat tgatccagcc
     7741 cagggcttcg ccgccggcgt tgcgcatgtc gaccagccag tggcggccac tgtcgaagat
     7801 catttcatca ggcgtgactt tcttgagttc cagggctgcc gaaccggtga tcagcttgaa
     7861 cgtagagccc ggcggatatt cgccggcaat gacgcggttt tccatggggt gattgggatt
     7921 gtcgttgatg agggaccatt ctttttccgt aatgccccgg gtaaaccagt tcgggtcgta
     7981 agccggacgg ctggccatgg ccaggacggc gcccgtattg gggtcgatgg cgacggcagc
     8041 gatgccgtgg gtgccgatgc cgttggcgat ctggcggtcc atggcccgtt ccgtagcctg
     8101 ctgcaggtag aggtcgatgg tcaggtgcat attgtgaccg gctacggggt ccttgcgttc
     8161 catttccttg accggactgc cggcggcgtc gacttcgacc tgacggccgc catccttgcc
     8221 gcgcaggagg tcatcgtaat aggcttccag gccggcccgg ccaatctggg tgatgttgga
     8281 tacgccttcg acgccttcca ggcgcttggc gtcttcttca tcgatctggc cgacgtagcc
     8341 aaagacctgg gcggccatca tcttgtacgg ataatagcgg atcggctgga cgtcgacgga
     8401 gacgccaggc aggtcgttgc gctgttcttc gattttcgtg acgatatcct gggtcaagtc
     8461 attggccagg accgtcggca cgtaagagct cttgaccttc tggacctttt cccgcagctt
     8521 atcttccggc atgttgagga tgccggccag tttctgcagt tcttcgtcgc tcatgccgtc
     8581 cttaggcggc acgtaggtca ccatgaagct cggccgcgag ccgacgagga tctggccgtt
     8641 gcggtcgtac atgataccgc gcatggctgc aatggggatg gcccgcaggc ggttcccttc
     8701 ggccagggta taataatatt caccggcaaa gagctgcaag tagaccagcc ggctgatgag
     8761 gatgacgaaa atcgcgatga cgatccagta gaggtagcgg aaacggccat cttgattctt
     8821 ctttttcaat aacgcttcaa gcatgtaact aaatctcctt tatttaccag cgatagcccg
     8881 ggaacccgta aaaggcatcg tcgcggcgca gatgccagac gatgtgatcc accggcaggg
     8941 ccaggattcc gtgataaaca agcataggga tgctgaattc caacaagtag gcggcgatgt
     9001 tgatgtactg gccggccagg aagaggaacc cgcaggtcag gaccaggccg acttcggtcg
     9061 ccccgaggac gatgagcaat gtcaataccc actggtcctt gtcgatgtcg cggccgaggt
     9121 agctgcagat gagggctacg atgagataga ccagcaggtg caggccaaag aaattgccga
     9181 taatgacatc ctgacaaaat ccgcccaaga gggccgtgat gatgccgatg cgctggccgt
     9241 gatggagggc catgaggacg acgaagacga agatgaggtt aggctggctc acgctgttaa
     9301 acataaaagg caggatggca gcctgcaaaa taaagacgat gaaggctgtc atgacacagt
     9361 tcagtttctt cataatgccg cggctccttt aattccttct actttgtcgc gattcgtctg
     9421 aggtacgagt ttcggcgtga cttccggttt ttcataggac ccggtatcgg ccgacttcaa
     9481 gatgacgaag acttcttcca gtttggagaa atcgacgctg ggacgaatga cggcatattt
     9541 tacgaaatca ttttcattct gatggatcga tacgatggtg ccgataggca ggcccttcgg
     9601 atagatggag ccgaagcccg atgtgaccag tgtatcccct tccaggatat ccgcatcctt
     9661 ggcgatgttg acgaactgcg gttccgtcgg cacgttgccg ttgccgcgga cgacagagga
     9721 aacgcgcgat tccggacgct gtacgatggc accgatgctg gtccgcgggt ccgtcaggag
     9781 ctggacgcgg gacgaatgcg gatagacgtc gctgatgaag ccgacgacac cactcggcgt
     9841 gatgaccggc atgtcttttt cgacgccttc gccgctgccg acatcgatga tcatggtgtt
     9901 ggtccacgtg ccgtagtcac gggaaatgac cgaaccagcc agaagctggt actgattatg
     9961 ggtcgatttg aaattgagga gcgcccgcag gcggtcgttt tcggctacga cttcatcgta
    10021 gtcgacagtc cggctcttga ggtcggcatt ttctttttcc agcgattccc attcaatgcg
    10081 gttcttcaag ctgacgtcga cgatgtggat gctcgaggtc agcgaatcca tgacgcggct
    10141 ggcaccgtat tcaaaaggcg acatgatcgt catcagcggc ttggtaatga acggcaggga
    10201 atcgcgctgg cgccaggccc agccgacaag cgcaatgacg atgaataaga tgagtacgaa
    10261 gacgacgctt cttttaccga acgaaaacaa ttttaaaatc tcctcctata tatacgctgt
    10321 gccccttcga tgagggccga taatttatca cgatcagcga cggcaatgcc cgtgcccttg
    10381 gcgacagcca gttccggctc ggaagccagc atgaccggca cgcccagttc ctgggtgaag
    10441 agctggtcca tgccctttag cttggccccg ccgccagtga ggacgatgcc atgttccata
    10501 atgtctgcgg ccagttccgg cggcgtttcc cgcaggacat ccttgaccag gtcgaggatc
    10561 ttgatgagag gcttgccgat gacgtggtag agctcactcg atttgatggc catttcccgc
    10621 tggaggccgt catcgaggcc gcggcccatg aagtaatagc cccggtcttc caggggcagg
    10681 acagctgagc cgttggtcac tttcatctct tccaccgtcg tgtcgtcagt gacgatatcg
    10741 tactgatagc gcaggtaatc tttgatggcc gcattgaaat cgaggctgcc cagatggagc
    10801 gacttggata cgacgatgcc gcccagggac agcatggcta cattggtcgt accgccgccg
    10861 atgtcgacga ccatattgcc gaccggatca tagacgggca gattcacgcc gagagccgca
    10921 gcagccgccg tttcgatgag gaaggcttcc ttggcgccgg cctgatagac ggcttccatg
    10981 acggcccgtt tttcgacatt cgacgccgcc gaaggcacgc cgatcatgat gcgcaggcgc
    11041 tggacgctgc ggacgcactt gctgaggata tagcccagca tcgtccgggt tacactgtaa
    11101 tcggcgatga cgccgtggac cagaggccga tggagcttca ccgtttccgg cgaccgggac
    11161 aataagtcct ctgccgcccg gccgacagca atcatcttgc tgttccgttc atctgtcgcc
    11221 atgaccgacg gttcagacag gacgatgccc cggccctcca catagacctg ggtcgtagac
    11281 gtccctaaat caatccctat atttttagtt aatggagaaa aaatcgaagc cataacaaat
    11341 cctttctatt tctttcgcat attctgttat acaaaacagt catcattcca tattctatca
    11401 cattgcgccc cttttcgcat taactaattt catggaatga aaaaaaggcc tgtcgcacgt
    11461 gcgacagacc cttttaagta gttagcagct agtggctagt ggctagcgga tggtctttaa
    11521 tttaaaaact ctcgcttcaa acatcaaact tcaagcaggg atacacccac atgtgcatga
    11581 cagccgaaac gaccacacgc ttcttacgcg actttcaatc cggcgcggtg caaggccatg
    11641 acgatcggcg tgatgatcag gcccgatacg atggcttccg ggatgccatt gacggccgtg
    11701 atgcccagca tgatgttgat gacgttcatg aaggggatgc cctgtgcttc ggcataaggg
    11761 acgccgacga tggccgtaaa ggaaccgagg aagagaatcg tgtgacagac cgtcgtcaag
    11821 accgccgtga cggcgatgcg ccatacttcg cggattttca gatgacggta caattcaaag
    11881 gcgatgacgc cgatcaggat acgcggtacg atacagatga aggcgtcggc aacgacgtta
    11941 tactgtacag caaacaacag caacgcgctc ggtgcctgca aggaccggat aaaggaaaag
    12001 ataccgaaca tgaagccgac gatcatgccg accttgcggc ctgccgtcag gctgccgatg
    12061 atagtcggga tgtgaaggat cgtcgcgttc atgatgacca gcgggatgaa accatagccc
    12121 gtgaggccta aaaaaatcgt aatcgccgat aagaaaccgg caaccgttaa ctgacgaatc
    12181 gacaaaaaag tgcgtcgtgc cggcatgacg ccagcaacat tctgtttttc catgtttttc
    12241 ctccgttctc gctctttcgc aagatacaag tcgcaatacg attaaagacg caaaaacacg
    12301 gccggtccgc gccagtatcc gcccgtttcc ggtaccaacc cggcgcttta aaagaacctg
    12361 ccttatgtgt cacgcgtagt atatcatact tgaaggggtc ttgccaatag taattgggaa
    12421 tattgtgaat ctttacacaa gcataatgga ggataaaggc cttcggccgc agttggcaaa
    12481 gaaacacggt ccagcaagaa cgggctgtgg atttttctcc agcaggctcg ccctctatca
    12541 tgaatcaaac ttcaatcctg aaaaagaaag ggactgtacg cgatagcaca aagcttcatg
    12601 cgacagtccc ttttggtgcg agtggaggga cttgaaccct cacgccgtga ggcgccagat
    12661 cctaagtctg gtgcgtctgc cattccgcca cactcgcaag taccttttta ttctaccatg
    12721 cttccctcct attggtcaaa caaaaaatat ggtatcatat ttctatatat atcataaagg
    12781 aggattgtta tgttattaca aagggaatcg aaatttgaag ggtctgtact cggatttatc
    12841 ggctatggcc tcatcttagt catccttacg atttgtacat tcggcatcgc ctatccttgg
    12901 ggcgaagtca tgttccgccg ctggatctgc cgcaatacga tcatcgacgg ccagcgtctc
    12961 acttttgatg gaacaggcct gcagctcttc ggcagctaca tcaaatggtg gttcttcact
    13021 atcatcacgc tgggcatcta cggcttctgg ctgttcaaca agatgaccgc ctggcgggta
    13081 aaacatacgc attttaggga ataataaaag nnnnnnnnnn gagttactaa ccactaacca
    13141 cgagccacta accacgagcc accaatctca cacgacctga aacaggtaga acttcaggta
    13201 attcgtttcc gggacattcc agaggatggg atggtccggc gactgctggc gcccttcgat
    13261 ctggcggagg cggacgccgg cgtctttggc ggcgtcgtgg agcatccggc ggaacaggtc
    13321 gtcacgcatg aagtgggagc acgaacaggt cgcgaggtaa ccgccgcggg gcaggagttt
    13381 catggctttc aagttgattt ccttgtagcc ccggatggcg ttgtggacgg tatgacccga
    13441 tttggtgaag gccggcgggt cgaggatgat gaaatcatag tcatggcact tgtggtcggc
    13501 cagatcggtc aagaggtcga agacgttggc ctgcttggcc gtgacgatgt cttccaggcc
    13561 gttgcggcgg atattttcgt tggtcatgtc gacggcttcc tgggaaatgt cgacggctgt
    13621 gacgctggcc gccccgccct tggccgcatt gagggcaaag gctccggtat gggtgaagca
    13681 gtccaggaca tgcttgccgg cagcgatttt ggcgacagcc aggcggttgt atttctggtc
    13741 caggaagaat cccgtcttct ggccattttc cacatcgaca tcgtagagga tgccgttttc
    13801 gtcgatggtc gtcaagacgc tgcccggctg gggcaggaag tcggcctgat accagccttt
    13861 atactcttcc atgccgtcca gttcgcggac tttgacgtcg ttccgttcat aaatgcctcg
    13921 gatggtgacg cccatggcgg ccagttcgtc gacgagagcc ttgaagatga ccggtttgac
    13981 ctggtccatg ccatagcaca gggtctgggc gacgaggacg ttgttatagc ggtctacggt
    14041 caggcccggc atgtcgtcgg cgtctccgaa gatgaggcgg cagcaggcga agtctttatc
    14101 gcccatgacg gtcttgcggt agtccagggc atatttgacc cggcgctgcc agaaagcttc
    14161 gtcgaagcgg tcgttggcat tgcgcgacac gatgcgcacg cgtatcttag aaatgtcgtt
    14221 ggcaaagccc gtgccgagat agcggtcttt gtcgctgtag acgtcgacga tgtcccctgt
    14281 ctggtacgtg ccttcgacat gggtcacttc ttcgccatat acccaggggt ggccgctgcg
    14341 ggcagcccgt tcccccttct tggtaatcgt taatttcgca taaggtcggt tcatatttcc
    14401 cccgttttat aaaatataat ggctcaagtc gacgttctgg acgacgtcgt tcagcttgcc
    14461 cgaaacgaat tctttattga cgacgatgtg cttttcgtcg atatccggtg cttcataggc
    14521 cacatcttcg aggatttttt ccaaaatcgt gtgcagccgg cgggcgccga tgttttccgt
    14581 ttccaagttg acgcggtatg cgtattcggc aatggcgtcg atgccgtcat cggtgaattc
    14641 cagggtgacg ccatccgttt ccaggagggc cttatactgt ttgaccaggg actggttcgg
    14701 ttcagtcagg atgcggcgga aatcgtcgac cgtcagggac tggagttcga cgcggatcgg
    14761 gaagcggccc tgcagttccg gaatgaggtc gctcggcttg gatacatgga aggcaccggc
    14821 agcgatgaag aggatgtggt ctgtcttgac cgggccgtac ttggtgttga ccgtagctcc
    14881 ttcgacgatg ggcagaatgt cgcgctgtac gccttcgcgg gagacgtcgg gcccgttgga
    14941 acggcccttt tcggcgatct tatcgaattc gtcgatgaag acgatgcccg attcttccgt
    15001 cgcttcgatg gctttgtcga cgatgatgtc catatccagg catttttcca attcttcttc
    15061 cttgaagatt tcccgggcct tggcgacggt catcttcttt ttcttcttct ttttcggcat
    15121 gatgctgccc agcatttcct ggaaattgtt ggtcagttct tccgtcgaat tgcccgtcag
    15181 gatgccctgg aagccctggt tctgttcagc cacttcgact tcgatttccc ggtcgtccat
    15241 cttatgggcg caaatgtctt caaacatctt ctgccggcgg tcggacttcg gcnnnnnnnn
    15301 nntttccttg atggacttct tcggccagaa gacctggagg atgcgcttgt tggcttcttc
    15361 ttcggccttt tcttcatagc gctggctttc ctgttcctgg accatgcgga tggcattggc
    15421 cacgaggtcg cggatcatct gttcgacgtc gcggccgaca taaccgactt cggtgtattt
    15481 cgtcgcttcg actttgatga acggcgcacc gacgagcttg gccaggcggc gggcgatttc
    15541 cgtcttgccg acgccagtcg gcccgatgag caggatattc ttggggatga tttcatcctg
    15601 catgtcttca ctcaggcgtt tgcggcgcca gcggttgcgc agggcgatgg agacggattt
    15661 cttggcttcc ccctggccga cgatgtattt attcaattcc gccacgatct gttttggcgt
    15721 caattctgtt ttttccatga aaatacctct ttcttaaatt tcttcgacaa tgacgttgtg
    15781 gttggtgtag acgcagatat cggctgcaat atgcagggat ttttcagcga tttcccgggc
    15841 cgacaagtcc gtgttctgca tgagggcccg tgccgaagcc agggcatagt tgccgccgga
    15901 accgatggcc aggacgccgt cgtccggttc gatgacttcg ccgttgccgg aaatgagcag
    15961 gatgcgctgg ctgtcagcga ccaggagcag ggcttccagc ttgtgaagga ttttgtcgga
    16021 ccgccagtcc ttggccagtt cgacggcact gcggacgagg ttgccattgt gttcgttcaa
    16081 tttttcttca aagtggtcga ataaggtgaa cgcatcggcg acggagccgg caaaaccgct
    16141 gttgatcttg ccgtggtaga ggcggcggac tttctgggcc gtccctttca tgatgacggc
    16201 attgcccatc gtgacctgac cgtcgccggc gatggccgtc ttgccgtcgc gctggacggc
    16261 gcagatcgta gtagcgtgga attctgtaga cattaattca gacatgggta atcccctttc
    16321 atttgttcaa tttcatcgag tgcccgctgt gccagcaggg cgttcttttc tttcttcttc
    16381 atgcgtttct tgatgcccag cggttccatg aggccgaagt tgatgttcat cggttggaaa
    16441 tggtcgttcg ggccgctgga aatatattgg ctcaggccgc ccatggccgt gcgccgggaa
    16501 aaggtcaggc cgtcttcccc ttgcaggagg cgggccgcat tgacaccggc caacaggccc
    16561 atggctgccg attccaggta gccttcgacg ccggtcagct ggccggcaaa gagcaggttc
    16621 ggctgtttgc gcagctgcag cgtcggcagg agcagttccg gcgaattgat ataggaattg
    16681 cggtgcatga cgccatagcg gacgaattcg gcgtgttcca ggccgggaat catggaaaag
    16741 acccgcttct gttcggggaa cttgagatgg gtctggaaac cgacgatatt gtacagcgtg
    16801 gcgctgccgt tatcctggcg gagctggacg acggcataag gcagttcacc tgtaccgggc
    16861 agttccaggc cgacaggctt catggggccg tagcgcatgg tatcgatgcc gcggccggcc
    16921 atgacttcga tgggcatgca gccttcaaag acgctgtcgt ctttttcaaa gtcgtggagt
    16981 tcggcacatt cagccgtcgt cagggcctgc cagaaagctt cgtattcttc tttggtgaaa
    17041 gggcagttga gatagtctgc cccacccttg ccgtagcggg cggcgcggta gactttagtc
    17101 atatccaggg agtcgacggc gacgataggc gcggcggcgt cgtggaaatg gaagtaatcc
    17161 tccccggtca ggcggcggat ggtatcggcc aaggcgtcac tggtcagggg gccgctggcg
    17221 acgatgcaga tctggttgtc cggcaattcc gtcacttctt cgtggcggat ggtgaccagg
    17281 ggatgatcct tgagtttttc cgtcacgtac tggctgaagg ggacgcggtc aacggccagg
    17341 gccccgccag ccggaacgcg gtgcgtatcg gcggcatcca tgatgagcga gccgagacgg
    17401 cgcatttctt ccttcaagag gccgacggcg ttttcaatat tcgctgcccg cagggaattg
    17461 ctgcagacga gttctgcgaa atattccgtg tggtgagccg gcgacgattt gaccggccgc
    17521 atttctacga gttctaccgg gatgccgcgg cggacgagct gccaggcagc ttccgatccg
    17581 gccaggccgg ctccgatgat taagacacta ttcttcatgc actacctcac cttttttatg
    17641 gttttcacac tctgcattgc tacagaaaat cttatcgggt ccttttttat aatgtttgac
    17701 aatcatgatg ctgccacaaa ggggacactt cttgtcgaca ggctggttcc agaggacgaa
    17761 gtcacattcc ggatactggc tgcagccgta aaagacgcgg ccccgcttgg acttacgctt
    17821 gatgagcgtc cccttgccgc acttgggaca ggtaatcccc aggtcttcga cgatggcctt
    17881 ggtattgcgg cattccggga aattggagca ggccaggaat ttgccgaagc ggccgaattt
    17941 atagaccatc ggcgcgccgc agagctcgca gacctgaccc gaatcctggc cggaaatggt
    18001 gactttttcc atcttttctt cagcttcgtc catttccggc ttgaagacgt catagaattc
    18061 ctggaggacg tggcggtacg tgtccttgcc ctgttcgatg gcgtccagct cttcttccat
    18121 gtgggccgtg aagccgacgt tgatgatctg ggcgaaatat ttcttcagca aatcgacgat
    18181 gatgacgccc agttccgtcg ggacgaactg tttatccttc ttttcgacgt aattgcggtt
    18241 ctggatggta tccatgatgg gcgcatacgt actcggccgg ccgataccct tttcttccag
    18301 cgtcttgatc aggctggctt cggtatagcg tgccggcggc tgggtgaaat gctgctgcgg
    18361 cgtgacggcc tggttgtgga cgacggtatg ggccgaaata tggggcagct cagccagagt
    18421 cttgtccttc ttggcgtctt cgtagagttc cgtaaagcnn nnnnnnnncc cttgaacagg
    18481 actttgtagc ctgcagcccg cagttcgtag cggccgctgt cgatgatgat ggccatgtgt
    18541 tccgtttcga ccgattccat ctggctggcc aggaagcggt tccaaatgag cgtatacagg
    18601 cgcagctgat cgcgggacag gaattccgac accttgtacg gtgtcagttc cagcgacgtc
    18661 ggacggatgg cttcatgcgc atcctgactg gtctgtttcg tcttgtagac gcggggcttg
    18721 tcggggatgt attcttcgcc gtagttcgac gtaatgaact cccgcgcggc ctggaccata
    18781 tcatcgttga tgcggaccga gtccgtacgc atgtaggtaa tgaggccgac atggccctgg
    18841 tcgcccatat cgaggccttc atagagctgc tgggccagca tcatcgtctt cttggcgccg
    18901 aaattgagct tgctgacaca ttcctgctgc atcgtcgacg tcgtgaacgg cggctggggc
    18961 tggcgtttgc gcttgcgctt ttcgaccttc acaatcgtat ccggttcgtg gtcttcgccg
    19021 cggctttccc aggtcaggtc gtgggcgatg gcttcggccg cttcctgcgt cgagatttcg
    19081 gccttcttgc cgtcaatctt ggtcagttct gtcggcaggg tcaggccgtc ttcggtctta
    19141 taggttccac tgacggtcca gtattcttcc ggcacgaaag ccttgatggc ttcttccctt
    19201 tcacagatga ggcggacggc gacggactgg acgcggccgg cgctgaggcc cttgcagact
    19261 ttcttccaca gaagagggct cagtttatag ccgacgatgc ggtccaggac gcggcgggcc
    19321 tgctgggcgt cgactttctt gaggtcgatg ggttccgggt ccttgatggc ctcaaggatg
    19381 gctttcttgg taatttcgtg gaacatgata cggcatttgt cgtgcgggtc gatttccagc
    19441 aaatgggcca gatgccagga aatggcctcc ccttcgcggt ccgggtcagt tgccagatag
    19501 acttgtttcg atcggatgta ttcattctgc aagtcttcaa tgagtttgcg cttatcccac
    19561 atattggtat acgtcggcac gaagccttct tcgatattga cgccgaactg gttgcgcggc
    19621 agatcccgca ggtggccgcg gctggccatg accttgtagc cgctgcccag gaagcgttcg
    19681 atagtcttgg ccttggccgg cgattcgacg atgaccagat tcttggcgtt cttcgggagc
    19741 gggtccagct tcttcagcgg ttccttcttg ggcagattga ccgtgccgtg gatgacggtc
    19801 ttctcgccgt cgcctttttt cttgcggcct cgtttcttct ttttagtatc atcaatgatg
    19861 attgtgcgtt taatcggaaa ttctaacatc tatctccatc ctgcctctcg ctgtacacga
    19921 aatctgaaaa tgtacttaaa ctatttctca tcatacacta ttcgccgcga cctttcaagg
    19981 tgtgactctt tctaatacta taatatgagc aaatatgagc acaatatgag caaatatgag
    20041 cacaaaaaag aggctgtcgc acgaagctaa aatggcttaa tgcgacagcc ccttttttag
    20101 tggctagtgg ctagcggctc cccttaaagg ggagctggcc gccgaaggcg gtctgagggg
    20161 ttgcttttga ccaaaaaaga ccctcgcatg atggtagaag ccttcgtgcg acaggccctt
    20221 tttgggtttt gagttttcag tttttagtga aaccgagtta gtgaatttca ctgatgatga
    20281 catcggtcat ggtctgggtg tcggctttct tgaagccttc tttatagagg tccggcgtgc
    20341 gccagccggc agccagggcg gcgtcgacgg ctttttcaat cttgtcggcc gcttcgtctt
    20401 catccaggga gtaacgcagg agcatggccg ccgaaaggat ggtggcgcag ggattggcaa
    20461 tgccctggcc ggcaatgtcc ggtgccgagc cgtgaatcgg ttcgtagagg ctggtcattt
    20521 cgccgatgga agccgacggc atcatgccga tggaaccgcc gaggacagcc gcttcatcgc
    20581 tgaggatatc gccgaagagg ttgctcgtga cgatgacgtc gaactgcttc ggattgaggg
    20641 ccagctgcat ggcgcagttg tcgacgtaga aatggttcaa ggcgacgtcg ggataatcct
    20701 tggcgacgtc gatgaccgta cggcgccaca ggcgggacgt agccaggacg ttggccttgt
    20761 cgacggacgt aaccttgtgg cggcgcttct gggcggcctt catggcaaag gtgctgatgc
    20821 gctgtacttc agggacactg tagttttcca ggtcccacgc gcgttcgacg ccattgtgga
    20881 tttccgattc gcagcggtcg ccgaaataga tgccgccgac gagttcgcgg acgatgagga
    20941 cgtccgtccc ggtgacgata tccggtttga gcggcgagta ttcggccagg acttccggta
    21001 ccttgaccgg gcgcaggttg acgtagagcc ccaaagcctt gcgcaggccc aggacggcct
    21061 gttccgggcg cagtttcggg tcgacgttgt cccacttgtc gccgccgacg gcgccgaaga
    21121 ggatggcgtc ggctttcttg gccgcttcga tggtatcttc cggcaagggc gaaccgcaga
    21181 ggtcataggc cgtgccgccg gccggtttct tttcaaaagt caggccgatg tggcagactt
    21241 catcgatttt ctctaaggct ttaacggcac ctgccgtgat ttcttccccg attccgtcgc
    21301 cggggatgac gacgatatgt ttagccatgt tacttagact cctttacgta attgatcaag
    21361 ccgccggctt tggcaatgtc ctggatgaag cccggcaggg gcggcgcctg gaaggtgtcg
    21421 cctgtcgtct tgtcgacgat tttccccgtg tcgaggtcga tggagaggat atc
//
LOCUS       DAWABZ010000019        20337 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_738_length_20337_cov_3.188295, whole genome shotgun sequence.
ACCESSION   DAWABZ010000019 DAWABZ010000000
VERSION     DAWABZ010000019.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 20337)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 20337)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20337
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_738_length_20337_cov_3.188295"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6905..6914
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8612..8621
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9190..9199
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15334..15343
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15807..15816
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16253..16262
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4539 a         5663 c         5814 g         4261 t
ORIGIN      
        1 tcctaaagtc atgcgcgttc actctttctg tacacaatag tctctgttgt acacattcaa
       61 tttcgtatac tatacaacac aatgtaatat acactgatca ttataatatg gaaatcatta
      121 taatatggat gataaaaagc gtcaaccgta tgattctaag aacttgcaag tattatgctt
      181 aaatataata ttgggacgaa aaatgatact taaattaaga aaaatattga taaaaacata
      241 gagatgtata cggagtatag aataaaaagc tatttatggc tgaaaaaggc acgaatcagg
      301 cggaccaggc tttcctggtc ggcagcgccc atcagttccc ggcacgagtg catggccgta
      361 atggggatgc cgatatccat ggcccgcagg gggagattgg ctgagagcag ggagcccagg
      421 gtcgagccgc cggggatgtc cgaccggttc atgaatacct gataggggat atcattttca
      481 cggcagaggg atttgacgac agctacggca gcggcgtccc cggcatagcg ctggctgctg
      541 gcgatcttca gggcgacgcc gcaccccatg acgggctggt tggtgacgtc gcatttttcc
      601 ggcacgttgg gatggatggc gtgggccacg tcgacagaga gcatgaaggc ccgggccagg
      661 tcggccgaga agtcatcgtc cgtcaggccg aaggcgtggt agatgcggtg caggatgtcc
      721 ggcaggacgt gggagtccgc cccttgtttc gtctggctgc cgacttcttc gttgtcgaag
      781 agggcgacga cgttgaggcc gtcggaagcg gctgtttcgg caaggcccgt caggcaggcc
      841 aggaccgacg tcagattgtc caggcgcggc gacgatacga attcatcgtg ccagcccagg
      901 cggcagcctt cttcataggg atagaccgtc aggtcccagg acaggatggc ttcgggcagg
      961 cagcggcatt tttccgacag gaaatcgagg aagaaatggt ccgtcagctc gcgctggtcc
     1021 aggcccatga ggggcagcag gtctttctgc gggttgagtt cgacaccttc attggcccgg
     1081 cggttcatgt ggatggccag gcggggaatg gtcagaatcg gtctccctgc gtcgacgagg
     1141 cggatttccg gctggaaggc atcgctgccc tggagggcca cggtcccggc cagggacagg
     1201 ggacggtcga gccacgattc gcgcagcagg ccgccgtaga cttcggcgtt cagcttgagg
     1261 tagccgtgct ggcggatttc cggccgatat tttattcgca gacaggggaa atccgtatgg
     1321 gccgcgctca ggcgcaggtg gcggcgcaca tcggaacccg tgcggaaggc gatgaggccc
     1381 gaatcataga gggggacgta ataggcaccg ttcggcgcca gttgccacgc ttcggacagg
     1441 gacagggcct ggaagccggc ccggtcgaga taatcccggc tggcctggac cgtatggtac
     1501 ggcgacgggc tgtgatggat aaaattcaat aagtcctgga tataatcata agtcatggag
     1561 gtcatccttt ctgacgagat aggtcggtta tttcaatagt agttcttatt atatcatata
     1621 tataagattc tatggaatga tatgtttttt tgcggcttcc tcgccagcca tggtataata
     1681 aaaaggattt tatttttttg aagggacgct tatggattta tacgtaatct gtcaattttt
     1741 aggaaggatc gcctgggccc aggccggcgt catgggtctg ccactgctca tggccctggc
     1801 tttcgccgaa gacagctggc cggcctttat gggcaccatg gccgtctgtg ccctggctgg
     1861 ctgggctttc ctgcgctatg gccacgtgcg gacgaagaaa ctgaccatgc gcgaaggcat
     1921 tgctatcacc ggcctgggct ggctcctggc aacttttttg gggatgctgc cttattgcct
     1981 cggcggctac ctgaactatt tcgacggcct gttcgagagc atttccggct ttaccgggac
     2041 cggggcgacg gtcatccccg acttggaggt cctgccacag agcctcctct tctggcgcag
     2101 cctgacccac tggctcggcg gcctgggcat tgtcgtcatc tttatcgccc tgctgccgga
     2161 agcgggccag tcgacggtct acatgtacaa cgccgaatcg acggggccca cgcgggagcg
     2221 ggttctgccc aggctccacg acatgaccca ggtcctgttc cgcatgtaca tggccttcac
     2281 ggcggcggcc ttcgtcatct tcctgctctg cggcatggat ttcctcggcg ccctgaacca
     2341 tgccttatcg acggtctcag ccggcggctt ttccacgtac aacgccagtg cggctcactt
     2401 cgagagcccg gccgtcgaag cctggatgac cttcttcatg atcctcacgg gcggcaacta
     2461 cggcctgtac taccaggcct ggcagaaggg ctggcacgtc ctgcggcgca atacggaatt
     2521 caaggcctac ctgttgatca tcgccgcggc gacagtcctc atcggcctca acctcatgga
     2581 cggcttcgac gtatcggcgc cgacagccct gcgctattcg tcgttccagg cggcgtccat
     2641 cgcgacgacg ggcttcgtct cggcagacta cgaccgctgg ccgccctttt ccaagggcat
     2701 cctgctggtg ctcatgattt ccggcggctg tgccggttcg acggcagcgg gcctcaaagt
     2761 ctcgcgcgtc gtcatcatgg tgcgctacat ctggcaggca gccaaagcca agctccatcc
     2821 tcatcaggtc tggggcctga ccatcaacgg ccaccgcgtc ggcgacaata cggtcgtccg
     2881 ggcggcccag ttttgcttcc tctacctgat gttcatcgcc ctctgggccc tgctgctgac
     2941 ctttgacggc atcaatacct tcgacgccat cggcatcagc gtgtccacca tgggctgcat
     3001 cggcccggcc ttcggcatca ccggcgcgac ctgtacctat gccgaactct cggtcttctc
     3061 caaatccatc ctctgcctgt ccatgctcct aggacgactg gaaatgttca ccctcctggt
     3121 catgctgcgc aagagcttct ggaagtccag gagcagctgg taaatgagag aaagattaaa
     3181 ttttgctgaa ttcaattaaa ggacgattaa attttcgaaa aggggttaaa aaatagggca
     3241 tactatacat atagattctt acagtatatg tataggaggg attgtcatga agaaattcgt
     3301 agctttggcc ctgattttgg caggcgtggt cctgttccaa ttcgcctctg actttgaaaa
     3361 cgcttttagt gagccaccgc cggccgtaga agagccattg ccggaaaaag cctttgtcta
     3421 tgtcacggga gccgttaaga atcctggcct ctatgcgctg gaaacgggga aaacggctgg
     3481 cgaagccgta gaagcagctg gcggcatgat tgcctatgcc gatacgggag ccgtcaattt
     3541 agcagaccgc gcagaagacg gtgcccatat ccacgtgccc tatgatttcc aggggattcc
     3601 cgaaaatccg gcggaagccg gcaaggtatc tttgaaccga gccgatgaga aagcccttac
     3661 ggacctgccc ggcatcggac cagctatggc ggctaatatc atcgcttatc gccaggaaca
     3721 cggctctttt ggcagtatcg aagaactaca gaaggtcaaa ggcatcggtc cggcgaagtt
     3781 cgccaagctg aaagatttgg tgtgcttatg atgggaaagg ttaaaccgga atgggtcagc
     3841 ctgtccctgg tcctggccct ggctgggggc attaccctgg cagatgtgct ttctttggct
     3901 ttgagccttt ggatttcttt gttcctgctg tctttgctgg gcctattctt tttccggtcc
     3961 cgcagccgta ttgccctagt tgtctgtctg gtcctggcgg ctcttagtct gggcgccggc
     4021 cgtctccagc tggaggcaga gcgctgcgaa gccctgccgc atcaggcagc tggtgccagg
     4081 ctgaccgtgg aagggacgct gcaagaacgg cgcagtacct atacgaatga aaagggacag
     4141 gtcggacggt acattatgca ggtccggcgc tattcctatg acggggaagc ggacgtacag
     4201 cctggaaacg gctgtatcta tgtgactttg ctggaaccgc aggtcgtagg cagtacggtt
     4261 gtcgtcactg gcgacagccg gcctttgaca tactataaaa atgacggcat gtacgacgcc
     4321 ctccatcgcg accgggaaaa agcgatttac ttgcggctgt ttgccgatga tggggaaaag
     4381 gtggtcgtga cggctccgcc aggtcgctgg gaggcctttt tgcaggattt gcgccaggct
     4441 ttgaccgggc gttatgaggt catcctcggc gagacgtatg caccggtact ggctagtctg
     4501 ctctttggcg gccattacga ggacttgccg ccaggtctgc tggaatcgtt cagtacgacc
     4561 gggctgattc atatattatc tgtttccggg tctcacgtgg ctttgctgct ggccgtcatc
     4621 cagctcatag gccgcgtcct gggattgcgg ccgcgcagct tgtgttgtat ttctgtgact
     4681 tttctcttct tatacggcgc gttgtcggat ttttcgtcgc ctgtcgtccg ggcgtccctc
     4741 atgggggccg ccagtgcctt gagcctgacg gcgcggcgcg aatacctggc cggtcatgct
     4801 ctggccctgg ctatggccgg gatgctgctt tacagcccat acctttgttt tgacttgagt
     4861 ttccgcctgt cctgtggcgc ctcggcaggc atcattcttt ttcagcggcc cgtaagctcc
     4921 tggtttgcgg gtctgccttt gtttttgcgg gattgcctga ctgtctgtgg ggcggcccag
     4981 attctggtcg tgcccctctt gttttcggcg ttattttctt ttcccgtcta ttcgctggtg
     5041 gccaatgtcc tggtcgcacc ggtcttggac ctggtcatgg tcctcggcct gttggcgtcg
     5101 gtcctgtccc tcttgtgggg cagcgctgcc gatatggtgt tgtggctcat caagcccttg
     5161 ctggtcctgg ctctgaaggg caatgccttc atcgccgcct tgccggggag ccgcttctgg
     5221 gccggtcagc cgtcgaagtg ggcctgtttg gcctggtatc tggctgtagt ctttgtcttt
     5281 tgcccctctt ttcgcaggcg catgggtccg cccttgctgg cctgtctggc ggcggcctgg
     5341 ttcctgcggc catccgggcc gggcgtcctg gtcctcgatg ccggacggga ccaggtgacg
     5401 gctgtcattc atgaggataa atcggccgat atatggtata ataaatcgag attcgccaac
     5461 cccgaccagg cagctgtcgt cgtgacgccg gctttgcggg ctcatggcgt cttccggctc
     5521 cgccagtgcc gtgtcgatgg cgacggggcg gatcatacgc tggccttgct gcggcgggat
     5581 tttaccctcc tgtccgggac ggggagcggc gatgtgcctt ttcgctgtct ggcgtcggtg
     5641 gccaggcctt ttccggcagg accgctttgt ctggaattcc ggtccctgac gggctggaat
     5701 ggccgggatt tccccgtatc ggccatggcg accattgtct atggctcgcg ccgcagcgat
     5761 gaaaccctga cagagtgggg cgagacggcg gccggttacg gtgtgccttg tttttcgcca
     5821 tcccgggacg gccaggtccg tctgtaccgc tggcgggggc agtggcgcat ttctacgttt
     5881 aatgaggaac agtcatggaa tataagaaac atataatgat tatttatggg acagaaccgt
     5941 atgtcatgga agagagtcgg aagcggttcc tggcggcggc ccacgagcgg gccggcgggg
     6001 aagccgagat tcagtctttc cagaaggatg ctgtggcggc gacggtcgtc gaatccttcc
     6061 aggggtcgag tctctttagc agtgggacgg tcacgatttg gtatgactgt ccctttctgc
     6121 cgctcaagcg cggcggccgg tcgcggagca aactcaccaa ggatgagcag tggttcctgg
     6181 acgaggcccg gcggctcagc gacgagaaca gtcttttgtt ctatagcaag ggcaagatgg
     6241 atacgggcag tgcttttttc aaggccctga agcccatggc cgacgtcgtg gcggccgaag
     6301 cggtgacgga aaagacggtc atgccttatg tgacggacta tttgaaaggg aagggcaagc
     6361 gcctgaccag ccgggccgtc gcctatttgc gggccctgtt ccagacctgg ggtgatattt
     6421 cgttgctcta tgtcttttcg gagctggaga aattgtgcat caccgtgccc gacagcagga
     6481 aaaccgtcga tgtggccgat ttgcaggacc tctttgccgg gacgatggaa aagaatctct
     6541 tcacctttat ggatgaattt ttgcggcgca atggggaaaa gaccctgcct tttttggaag
     6601 gcctgttcag ccggcaagat gcttttttga agaatacggg ctatatgttg tcgcgcctgc
     6661 gcctgctcct ggcttataag gaattgcgcc gggcccatgc cggccagcac cagtgcgaac
     6721 agatcctgca gaccatcaat aagggccgca gcgtaaaata tgtcttatac cacttgcaaa
     6781 aagtggcatc ttattggaaa atagaagagt tggaaagttg tatttgccaa atttttacgc
     6841 tccagctcca tatccgccgc ggcacggcgt cgccggccga tatgggaccc cttatttgtt
     6901 tgtannnnnn nnnngcctac ggcggcccgc aggtcgcagg tcgcagttcg cctcccctca
     6961 taggggaggt ggcctgaaag gccggagagg ttgatttgcc gtttgcagga gcagttgaaa
     7021 tatagagaaa aggtaggtta gcatgtgaga gatgtagggg cgaccacgtg ccttttcagc
     7081 ccgtcaggtc atgactgtaa ggaatggcag gacggttgct ggaacggaca gctgaggctg
     7141 gcggcccgca ggtcgcaggg gagaacctct cagtcagctt cgctgacagc tcccctatga
     7201 ggggagccag gggcgattga aatatagaga aaaggtaggt tagaatgtga ctgacgaaga
     7261 acgagcacgg aaagaacgga tgaaggaata tcgccggcgg taccgggaaa agcatggatt
     7321 gcctccggaa aaggacacgc cgtcctggaa gaagcgggct gctgttgtct tggccttgtg
     7381 tgtgattggt ttcggcggct tttttgcggc ccagagcaag gcggaacaga tggtgaccct
     7441 caatgggcaa tctattgccg atatgacgga tgacgagatc accaattatc tcgacctcca
     7501 cgagaaggaa gcggaagacc gcaagctgcg cctggccgcg gacggcgtcg atgtgacgct
     7561 ggacctggac gaactgggag ccaaattgga ccgcagctat atcgatgatg aactgcatct
     7621 cgtcggccgc cggggcctgc cctggcagcg cgtggccgat gtggtgacga cgctcacgca
     7681 gggcaaggat gtgcccctgg ccattgccgt tgacgaggat aaattggata aggccctggc
     7741 cgatgtccac gacaagtacg ataaggatcc gcagaatgcc tatgcctatg tcaaggacga
     7801 ccagaagact gtcgccattg aagatgccaa ggaccgcatc gtcatcaata cagataagct
     7861 gaaagatgct gtgacggcca aactgcatac gggcaggacg gacacgctgg acgtccccgt
     7921 agacagccgg gaaggggcgg acatcaaggc cgacgacctc aagaatatcg acacggtctt
     7981 gtcgtactat acgacccatt tcgatgatac caacccggac cgcaacgtca acatccgcct
     8041 ggcacagaag cgcctcaacc acgccgtcgt catggcccag aaggatttct cctttaacaa
     8101 gtatgtcggc acgcggacgc cggacaaagg ctataagcag gcaccgtcgt attttgaaaa
     8161 caagctcgaa cagtcgacag gcggcggcgt ctgccaggtc agtacgaccc tgttcaatgc
     8221 ggctttgcgg gcgggcttgt tcattgccag ccgggaaccg cattttgccc cggccgctta
     8281 tgtgcccgtc ggcatggatg cgactgtcgc cgatgacggc ctggattttg cctttaccaa
     8341 tcctttccag catccggtct gcgtctatac cgtcgccggt cagaatacgg tgacgacgta
     8401 tatcctgggc aatcacgccg atacgtgcag tgtgtccttt gaaacgacgc atctccagaa
     8461 cctgcctcat aaggtcatca agaagcacga cgacagtgtg accgaagata agcgcaagca
     8521 ggaaggctac gacggccatg acgtgactat ccgccggacc gtggcttaca gcgacggcga
     8581 ccgccatgtc gataccatcg agtcccatta tnnnnnnnnn ntatgaaccg aatgatgaaa
     8641 tcatcctcac gcccggcgac gatagcgaag aagtcgtcag cacggcggac ctggaaccgc
     8701 aggacccgct cctcaacgca ccgcacgaca tgatggactt caatgtcccg gcgcccgatg
     8761 cggtcaacac ggccgatgag gaataactca tcggttcatg tagtaggggc tcgcacgtgg
     8821 cgagtccgtc aggtcatgac ggtaagaaat ggcaggatag ctgctgaaac ggaccgctgt
     8881 agagggtgag tccgtttgga ttttgtagga atttcaccaa caggtagcga atgttcccac
     8941 actgtgtgcg acgggcgtcc cacgtggacg cccctaccca tggatgggaa gcaggggagg
     9001 catgtagggg ccgtctcagt tcagtggtta aaatttaaag ccattgtctg caaactccaa
     9061 actataacgt aaaagggacc gcgcatggtg gtaaaaactt tgtgcgacag gcccgcttat
     9121 agaaaggaaa atatatgaaa gtctggcata ttgttcctaa aaatgatatt ttgatccccg
     9181 tcgacggcgn nnnnnnnnnc ggtcgcttcg attgcggccg atctggccgg tcatgcctgg
     9241 catgtccgga cggaatcgcc ttatgccatc ggcgatatgg atcttctccg cgaccgggtc
     9301 agccggaaat tgggcctgga cgggacggta tcggtcgaac accgggtgac gtcggtcttt
     9361 cacggcggcg atatgtctct ggccgtgccc gacaatgacc cgctgcgcca ggtcgccgat
     9421 atttccactg atgatatgga aggctacggg attcctcatg aagactgcta cgattccatt
     9481 tacgatgtgg acgacgaatt gtacgccgat gaagattaca agaaggcctg ccagtcggcg
     9541 tcccagcccg gtacgggcag taccaggacg ggcggcaagg ccagcggcac caagaccggc
     9601 ggcgacagcc gggtctggat gggccgggcc tttggcggcg atgccgtcgc catcaacgac
     9661 gtcctcggcg aggaacagaa cgacgttatc ctcaagggga aggtcgtcaa ggtcgagttc
     9721 cgggaactca agtcgaagcg catcctcctg accttccaga tggccgacag caccaacggc
     9781 atttcagcca agaagttcct cgacgtcagc aatcagggcg gaggcggcaa gttccgccgc
     9841 aagaacacgc tgacgccgga agaatacgac aacctggtca agaagctcaa gcccggcgtc
     9901 tacgtccggg tccacggcaa tatccagtac gacaattacc agaacgacta cgtcctcatg
     9961 gcctacgaca tgatggaagc cgacggcggt acagtggaac gggaagacca taatcccacg
    10021 ccgcgcgtcg aactccacct gcatacggtc atgagcgaca tggacgccct gatcacggtc
    10081 aaacagctca tcaagaccat caagaagtgg ggccatccgg ccgttgccgt caccgaccac
    10141 ggcgtcgtcc agtccttccc gctgctccag gaaatctcga cggacaagac caacaacgtc
    10201 aaggtcatct atggcatgga aggctacctc ttcgatgata agatcgacca gtcgtaccat
    10261 atcatcatcc tggccaagaa ccagatcggc atccgcaatc tctataaatt ggtcagcatt
    10321 tcccatttga aatatatcta ccgcggccgg ccgcgcatcc cccgggcggt cctgtcagaa
    10381 taccgcgaag gcctcatcct gggctcggct tgcgaagccg gcgagctggt ccgctcgatg
    10441 gtccagaaga agctgcctta tgaagaattg aagaaaatcg catcgttcta cgactatctg
    10501 gaaatccagc cgctcaccaa caacggcttc ctcgtccgcg aaggcttcgt cgccgatgaa
    10561 gaaggcctgc gcgacatcaa ccggacgatc ctcaagctcg gcgatgacct ggggaaattg
    10621 accgtcgcca cctgcgatgc tcatttcatg aaccccgaag acaagattta ccgggaaatc
    10681 ctcatgactg gcaagggctt caaggatgcg gaattcctgc ccgacctcta cctgcgcacg
    10741 accgacgaga tgctggctga atttgcctac ctcggcgagg aacgggcgcg ggaagtggtc
    10801 attaccaacc ccaataagat caacgatatg atcgacgact gccggccggt gccgaaggaa
    10861 acgctctatt tcccgcagat cgccggttcg tcggaagcac tgaagaacat gtgctataaa
    10921 aaggcccatg aaatctacgg cgacccgctg ccgaagattg tcgaagaccg attggaagaa
    10981 gaattcacgt ccatcctggg ccacggcttc ggcgtcctct attacatcgc ccacaagctg
    11041 gtcaagcact ccaacgacga cggctacctc gtcggctccc gtgggtctgt cggctcgtcc
    11101 ttcgtggcga ctatgtcgga cattaccgaa gtcaaccccc tgccgccgca ttatgtctgc
    11161 cccaagtgcc agtggagcga gttctttacg cacggcgaag tcggcggcgg cttcgacctg
    11221 cccgacaagg tctgcccgaa gtgcggcacg ccgctccaca agaacggcca caacatcccg
    11281 ttcgctatct tcctgggctt cgacggcgac aaggtccctg atatcgacct caatttctcc
    11341 ggcgaatacc atccgaaagc ccataagtac acggaagaac tcttcggcaa ggacaacgtc
    11401 ttccgcgccg ggaccatcgg tgccgtcaag gacaagacgg cttatggcta cgtcatgaag
    11461 tgggccgaag ccaagggcat caaggtcaac gatgcctatg tcaacagcct ggtcgccggc
    11521 tgcacgggca tcaagaatac gacgggccag catccgggcg gcgtcatggt cattccccgc
    11581 gatatggacg tccatcattt cacgcccgtc cagtatccgg ccaacaagaa ggacagcggc
    11641 atcattacga cccatttcga ttatcactcc atcgaaggcc gtctggtcaa gctcgatatc
    11701 ctcggccatg atgatccgac ggtcatccgc atgctcgaag atatgaccgg catcaaggcg
    11761 acgactattc cctttgatga cccggcgacg ctgagcctgt tcagctcgac caaggccctg
    11821 ggcgtgacgc cggaagaact gggctcgaaa gtcggcagcc tgggcatccc ggaattcggc
    11881 acgtccttcg tccgccagat gctcgtcgac accaagccca agaccttttc cgaactggtc
    11941 cgcatttccg gtttctccca cggcaccgac gtctggctga acaatgccca gaatctgatc
    12001 acgtcgggga cggtccccct gaaggaagcc gtctcgacgc gtgacgacat catgaactat
    12061 ctcatccagc acggcatcaa gcccaagacg agtttcaagg tcatggaaaa tgtccgtaag
    12121 ggcaagggca tcgacaagct caataaattg ggccagaaga cgacggacta cgaaggcgag
    12181 ctcaaggccg gccacattcc gcagtggttc atcgactcgt gccacaagat cggctacctc
    12241 ttcccgaggg cccatgccgt ggcctacgtc atgatggctt tccgcatcgc ctggtacaag
    12301 attaaccagc ctctggccta ctactgcgct ttcttcacga tccgcgccaa ggccttcaag
    12361 ctgtcggcca tgatccacgg cctggaaggg caggaacggg ccatgaagga aatcgccgcc
    12421 aagggcaaga gtgcgtcgaa aatcgaacag gacctctatt cggccctgga actggccaag
    12481 gaaatgagcc tccgcggctt ttcctttatg aatgtcgaca tcaaccgctc ggcagcgacg
    12541 aagttcacca tctgcgacgg caagatcctg ccgcccttta cggccatcga cggcctgggc
    12601 gccaacgtag ccgaacagat cgtatcggcc cgcgaacagg cgccgttcag ctccaaggcc
    12661 gacctcaaga tgcgcggcaa agtgtcccag tccctggtcg acgtcatgag ccaggaaggc
    12721 tgtctcggcg atttgcccga agatgaacag atcgacctct tcgccatgta ggcggaaaaa
    12781 acatttcctc gacagattga ctttcgtgcc gtataatgtt tttagtattt aacgaactaa
    12841 aggaatctgt cttagaaaga gggattgtca tggcagtgaa tgaaaaattg cgagaccatt
    12901 tgcacgatca gttatttcgt gctattttgg aattgaagag tgtcgaagaa tgttatgaat
    12961 tttttgaaga cctttgcacc attcaggaaa tgaaggccat ttcggcccgc ctggaagtgg
    13021 cccgtatgct caaggccggc gatatctatg aagacatcgt caagaagacc ggcgccagca
    13081 cggcgactat cagccgcatc aagcgctgcc tggtctacgg cagtggcggc tacgagaaga
    13141 tcctctcccg catggccgaa aaggacccgg atttcctgaa gttatccaag taggagggta
    13201 gccgatttca tgaaggctaa gattgatgtc accattttcc ataatggtga tatggatata
    13261 ttgcatgctt ccatttacga agagttgtgg aaggattatt gtacatttaa aaaacgggct
    13321 gccatgcagc aggacaaggg gaccaagaag ggcacgttcc tggcccggcg ctattaccgg
    13381 gcggccctct tgtccctgtt cgctttcttt gaaggcgtct tgaacagctg ggtcaagacc
    13441 gtcatccagg aacggcagga atttgccggc gtcgaacggc aggatacgct caagaagtgc
    13501 gacgccatgg tggaatactg ctttttctgc tcctatacca agcggcccgg tacgttttgt
    13561 tcgctttacg gctatatcaa ccgctatgaa cagcacgacc tggccctcat cgagcacatc
    13621 gacggccaga cgctggggca gatcgagacg gccatggaag agttcttctg ttacgtcgaa
    13681 gccatgaccg ccctgcgccg cttcccgaaa ccgaacgagt cgacgacggg cctcgtgagc
    13741 cgccttggcg ggatggtaaa ggattgccgg ggctgaacgg aaaggatgtg ttacctatga
    13801 gtgacaagta ttacctcggt ttcgatgccg ggacgcagag cgtcaaagtc gccgtctacg
    13861 acgaagacca gcactgcctg gctatggaca cggccaagac ccatctggcc tacccccagc
    13921 cgggctgggt ccagatggac gtcgatgaat attaccgcct catcaagcag tgcatggctt
    13981 cgtgcagccg ccagatggaa gagaagggct gggacgtgaa gaacgtccgg gccatcatgg
    14041 gcgacgggat catctgcggc atcgccggta tcgacgccga cggcaacgcc atcacgcctt
    14101 atgtcaacta tctcgacagc cgcacggccg acgacgtgga agccctgaag gcgcagaagc
    14161 tggccatctg gggcaaagaa acgggcaatg ccgaaccgaa catcatgttc ccggccatgt
    14221 tcgctcgctg gttcatgaag aacgtcccgg cctggcagga aaagggcgtc aagttcgtcc
    14281 acaactgccc gtacgtcctc atgcacctgg ccggcctcaa aggggaagac gccttcatcg
    14341 actggggcgc catgtccggc tggggcctgg gctatgacgt ggtccagaag aaatggtcgc
    14401 cagaacagct ggatatcctg ggcatccctg aacgctatat gccgcgcatc gtcaagccct
    14461 gggacatcat cggcaggctc tgcgaagaag atgccatgga aacgggcttc cctgcaggta
    14521 tccccatctg cgccggcgcc ggcgacacga tggaatccat gatgggcagc ggcatcctgg
    14581 aagcgggccg cgccgtcgac gtcgccggga cgtgcgccat gttctgcgcc gccacggacg
    14641 gcatcattcc ggaactgtcc gtgccgggga gcaacctcat cttcaattcc ggcaccctgc
    14701 cggggacgta tttctactgg ggcatggtcc gcacaggcgg cctggccctg cgctggttca
    14761 aggacatcat tgcccgcgaa gaaggcgatg actactacgg gaccctggac gaactggccc
    14821 ggcgccatca gcccggctgc gacggggcca tgttcattcc ctacctgacc ggcggcaacg
    14881 gcgactatcc caacgtccgc gggaccttca cgggcctgac cctcgactcc gaccaggggg
    14941 ccatgtggcg ctgcgtcctc gaaggcatcg gctatgacta tatggaaatc accgaccgct
    15001 accgcaaggc cggcatcgac ctgacccagc tgaccatcac cgaaggcggc agccgcgatg
    15061 aactgtggaa ccagatcaag gccgacatgc tggatacgaa aatcgtgacc ctcaaagtcg
    15121 ccggcggcgc cgtcccgacc aactgcatcg tcgccgccta tgccgcaggc gacataccgg
    15181 acctcaaaca ggccctccag cgccagctgg aaatcaaggc cacctacgtg cccgacgaaa
    15241 accatacggc tacctaccgc agggcctatg acaaacgcga aaacctgctc aaagccctga
    15301 acggcacttt gtgagtcgtg gctcgtggct cgtnnnnnnn nnncgtaggg gccgcccaaa
    15361 gggcggcccg cctgaatccc aggagcaacg ctatccgtaa ttcgccggcc gcttcggatt
    15421 ccgatgcggc ggcccgccgc gacagactca aaatgagaac gacgcttcgg gataccctca
    15481 gcgcgtagcg ggccgccctc tgggacggcc cctacgaatg ccgttgcacg gcagcgcctc
    15541 aaagggaaag catcgccttg ggtgattttc gactcgtaaa aatggccccg attggggctt
    15601 ttcgtagggg ccgtcctgat aaggcggccc gcctgaatcc cgtgaacaac gctatccgaa
    15661 gggcggtccg ttcggctctc ctcataggag agctgtcagc gaagctgact gagaggttgt
    15721 cctttcgtga tagtttatcg tacggagttt gtagtttata gtttgtagaa gttggtttta
    15781 aattttttac catttgctgc aaactannnn nnnnnngtct taatgcgaca gcctcttttt
    15841 tgcggtattc ccacaagcgg gccatccgta aacggaattc ccacaagcgg gccgcccgcc
    15901 acggctgtcc gttccagcag ctgtcctgtg cctcccgttg gtcagcacct gacgggctgg
    15961 aaaggctttg gggcggcccc tacatgcgac atctttgatg tcgcggcgac ctgcgggtgg
    16021 ggattcccaa agggaatccc tacccatgca ctttgtgaaa aatagaagta tctcccatga
    16081 cgtaagttca taacgtcaca tagatctatg agataattct atcgaatgat gcaaagaagt
    16141 aatttgactt cttgattatt gcataaaacg catgtcataa atgtgaaaaa ttgaaaaaag
    16201 tgccgttttt tagtggattt ttttgggaag tgtggtatac tatcagtgta agnnnnnnnn
    16261 nntgaatctt atttagaacg tgcgtgattt ttttagaaga atctagtgaa aaaaatcacg
    16321 cgacgaagga ggagttaaac atgagtaaag taatggttat tggtgcaggt acaatgggtt
    16381 ctggcattgc acaggttttc gcaacgcacg gctgggatgt cgtcttgaac gatattaaag
    16441 acgaattcat cgctggcggc aaagctaaaa tcgaaaaaca gctgaccaaa caggtctcca
    16501 aaggcaaaat gacccaggaa gacaaagacg caactatggc tcgcgtttct ggcttcgtaa
    16561 cccgtacggc tgataacatg aaagacatcg acctcgtcgt tgaagcagct atcgaagact
    16621 tgaagatcaa atcggctatc ttcaaagaac tcgacgaact ctgcccggaa cacacgatcc
    16681 tcgcatccaa cacttcgtcc ctggctatca cccagatcgg cgcagctacg aaacgcccgg
    16741 acaaagtcat cggcatgcac ttcttcaacc cggcaccggt catgaaattg atcgaagtca
    16801 tcaacggcat cgctacttct gaagaaacgt atgaaaagat cgacaaactt tcgaaagacc
    16861 tcggcaaaac accggtcaaa gttaacgact tcccgggctt cgctggcaac cgtatcgtca
    16921 tcccgatgct gaacgaagct atccaggctt tgatggaaga cgttgcttcc aaagaagaca
    16981 tcgataacgt ctgcaaactc ggcttcaacc atccgatggg acctctggct ctctgcgacc
    17041 ttattggtaa cgacgtcgtt ctccacgtta tggaagtcct ctacgaaggc ttcggcgatc
    17101 cgaaatatcg tccttgcgca ttgctcaaga aatatgtcca ggcaggttac ctcggccgta
    17161 agactggcaa aggcttctac gattacacga aataagttag cgatctgcta atcgaatacg
    17221 gggcgtgact ttcacacccc gtattatttt tagaatctca tgcgcgtatt gacagtgcgc
    17281 cgatcctgtc ggcaggggcg gtctgactgg caggagtccc tttggggagg gcagcctgtt
    17341 gtacatacta tttctatact atactattaa cactattata tgtatttagg aggtttttct
    17401 catggattac cagaacattc tttttgctgt cgaagacggt attgcaacga tcaccatcaa
    17461 tcgtccgaaa gctctgaacg ccctgaacca ggctacggtc agcgaattga aggacgttgt
    17521 cgaaaaagtc gcagttgata aagctatcaa agtcgtcatc atcaccggcg caggtgctaa
    17581 atccttcgtc gctggcgctg acatcaaaga aatggcttcc aagaacgctg ctgaaggccg
    17641 cgaatggggc cagttcggtc agaacgtctt cacggaaatc gaaaacctgc cgcagcctgt
    17701 catcgcagct atcaacggct tcgctctcgg cggcggctgc gaactctcct gcgcttgcga
    17761 tatccgctat gcagctgaaa acgctaaatt cggccagccg gaagtcggtc tgggcatcac
    17821 cccgggcttc ggcggcacac agcgcctgac ccgtgtcgtc ggccgcggcc acgctaaaga
    17881 actcatctac acgggcggca tgatcgacgc tgaaaaagca aaagctatcg gcttggtcaa
    17941 cgaagtcttc ccgcaggaag aattaatgcc ggctgctgtt aaactggcta agaagatcgc
    18001 taagaacgct ccgattgctg tacagctctc caaagctgcc atcaaccgcg gcatcaactg
    18061 cgacgtcgta accggtatcg cttatgaagc tgaagtcttc ggcctctgct tctccacagc
    18121 cgaccagaag gaaggcatgg ctgctttctg cgaaaaacgc aaagcaactt tcgaaggtaa
    18181 ataattccat tgtttgactc ctaatagaaa agggcctgtc gctatgcggc aggccttttt
    18241 ctgttgagca acctctcagt cagcttcgct gacagctctc ctataaggag agccacgagc
    18301 cacgaattac gagccactag ccttttcaca aaattgtgtt acaacggcca caggaaggcg
    18361 aaccaggcgt ggtataatga attgcaaaaa aatttcaatt cgaggtgacc tcttcatgaa
    18421 aggtaaacat attcccgctc tggctgtgct ctgctgtgcc ctcagcctga cggctcctgt
    18481 ctgggccgca ccggcagtgg atggcttaga tcagacggcc gtgacggcga cggcagcggc
    18541 gacagcggct ccatcttcta tccgcgacgt gacggccttg agtacggtct atgatgacgg
    18601 cgaaagcgta tcggctgtag cccttcatta tcccaagacc attgatgcca gccgcctgaa
    18661 ggcgtcggat ttcaccgtcg ccggcaagac gatcgacaag gtctatacca atgatgcgcc
    18721 ggaaacggcc ctggccggca agaagggcag ttacgtcatc gtcagcctag tccatccggc
    18781 tacgcccttg ccgacggcag cttcccagaa gaagcccaaa gatgaccagg gcgataaggg
    18841 caaagaaggc tccaacgcct tgaacggcgg cgatgcgccc atgtattccg accgcaaggc
    18901 accggacctg agcgtcgctg tcagccagac cgggacgatc tacgcgacgg atggcacggc
    18961 ttatggcccg acgacgacgg ccctggctgc cgacaagacc gtcgatgccg tcaaggacgc
    19021 ctttaaaact tatatatata aggacccggc tacgggtata taaggacccg gctacgggct
    19081 atgaaatgcc gtataatatt tacctgccca aagattacga cgccgacaag gccaagactt
    19141 atcccatggt cgtcttcatc gccgatgcca gcgccaacat caacgacccc aaggccgtcc
    19201 tctatcaggg caacggggcc atcgtctggg caacgccgga agaacaggcc aagcatccgg
    19261 ccatcgtcct ggcgccgcag tatacggaag acctcatccg ctctatcggc atgatgacca
    19321 ccgataccca ccagtggacc cagggcctga ccctggtgac ggacctcatc atggatgtat
    19381 ccaataagta ccgcgtcgac aaggaccgca tctacgggac gggccagtcc cagggcggca
    19441 tggccaacat cgccatcagc gacgcctatc cggaactctt tgccggccag tacctcgtcg
    19501 cctgccagtg ggatacgcag gaaatggaaa aactcaaggg caagaacctc tggatcaccg
    19561 tctgccaggg cgacagcaag gcctttccgg gcatgaacga agcgacggcc ctgtgggaaa
    19621 aggacggcgc tgtcgtcgcc cgcagccgca tgtgggacag cacgggcggt acgcctgtct
    19681 ttgaacagat gatgcgggtc atggaagacc agcaggcacc catcaactat acggtcttca
    19741 aggacggcag ccacatgtat acctggtcct ttgcctacaa tatcgaaggc atccgggact
    19801 ggctctttga acagaccaag gccgaagatg tcctgaagaa aaaggcaccg gccggcatgg
    19861 atgccaaaca gcacggcaag gacggcgccc ataagggcag cccccagggc aaagccctgc
    19921 tcaacacggg tattgcctac ttgtacggcc aggacggtgc gacggactac gacgtcgccc
    19981 gcgaatgttt ccagaaggcc tggaacgacg gcaacatgaa ggccggccgc tacctgggcc
    20041 tcatggccgc taacggcttg ggcgaaaaac agagcgatgc ccaggctgcc cagtggtacg
    20101 acaaggccta gcagaaaggc gatacgacca gtatgtatct tttgggcaag tgctacgaac
    20161 agggcaaggg cgtcaaacag gattacgcca aggccaggag cctctacgaa aaagcggctc
    20221 agcgcataaa gcctccctta ctaggggagg tggcccgaaa gggccggaga ggttgaattt
    20281 cactgtaggg gctcgcacgt ggcgagcccg ctgggtatat gtgagatact tgtgacc
//
LOCUS       DAWABZ010000020        18690 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_815_length_18690_cov_3.593024, whole genome shotgun sequence.
ACCESSION   DAWABZ010000020 DAWABZ010000000
VERSION     DAWABZ010000020.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 18690)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18690)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18690
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_815_length_18690_cov_3.593024"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8040..8049
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14691..14700
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4281 a         5273 c         5021 g         4095 t
ORIGIN      
        1 gaataaaaaa agagagctgt cgcacaaagc cgaaatggct taatgcgaca gccccctttt
       61 tttagtggtt agtagctagt ggctaggggt tagcggaggt tttcaaattt aaagccatct
      121 tctacaaact ataaactaca tactacaaac tataaagtga aaaggacctc gcatgatgac
      181 aagccccttt ttcgacagcg ggcgccctac gtggcgctcc tacagggaat accgcttgaa
      241 agcggtattc ccttgcaccc cttcctcacc tcgtgtatac ttaggataat atgattgccc
      301 caggcagagg aggtcttagt atgacaaaaa cgcagcatgg tattgtggct tgtgtcatcg
      361 gtgcgacgac gtgggggttc aatggcgttg tcagtcagtt cctgctctct cattatcctg
      421 tcgagccgtc ctggctggcg tcgatacgca tgtgcctggc cgggttgttc ctgacgctca
      481 tggtactgcc gacgaagcgg gccgagatac gccagatgct gcatgacccg gtcagtctgc
      541 gccacttggt cctgtttgcc ctattcggcc tcatcttgag ccagttctcg tatttatcgg
      601 ccataaagaa ctcgaacgcc ggcacggcga cggtcctgca gaccctgagt gtcgtcctca
      661 tgtcgctcta tttagccgtc cgcttccata aacggccgac agggcgggaa atcctgtcgg
      721 tcttcctggc ctttggcggc gtctacctgg ctgcgaccaa cggcaacccg tcgacgatgg
      781 tcatttctcc gttaggcctg acctggggcc tcatcggcgc catcgcctgt gtcacctatc
      841 ctgtcccagg ggctggcagc tcattgggga gcggccgttg ccaacggcct gggcatgatc
      901 atcggcggcg tttttctcag tctgaccctg caggtctgga cgatctggcc cgcattggac
      961 ctgacgggct ggctggctgt tgcctttatc gtcatcatcg gcacggccct gtcttttacg
     1021 gcttttgtca cgggtatcag tgaaatcggg ccgatgaagg cgactctcct gggcacgctg
     1081 gaaccggtcg tcgcttcagt gacttcggcc atcgccttgg gtacgacgtt ccatacggtt
     1141 gaactcatcg gctatgcctg tatcatcgct acggtcttca ttatcatctt gcagaagaaa
     1201 ccagctattt cgtaatacgt acttttcgtg tataataaga gatgaatgaa taatgttttt
     1261 ttcatctttt gagaaaggtg tgttactatg attattgatg atgccggcgg cctcgtcttc
     1321 cgcccgccca gtgaagcccg cagcttcatc ctgcgcgtta ctatcggctg ttcccacaat
     1381 acctgccgtt tctgcgatat gtacaaagcg tctaaattcc gcatccgtcc catggaagaa
     1441 atagaaggga tcatcgagcg cggcgcctat gccatgccct tcatccgccg gattttcctg
     1501 gctgatggcg atgccctggt cctgccgacg gacaaactcg tagctatcat gcagaaatgc
     1561 tacgaaaaat tccccaatct gaaccgtatc ggtgcctatg cgactccggc tgacctggtc
     1621 cgcaagacgc cggaagaatt gaagcgcctc cgcgaagcgg gcctggccat cgtctacatg
     1681 ggcattgaaa gcggagacga cggggtcttg aaactcgtcg ataaagggac gacggcagcc
     1741 ctgaccatcg aagccgggaa aaaagccctg gcagcaggca tgaagctgtc gacgatgatt
     1801 ctcctcggct tgggcggcca ggaacggacc aaagaacatg ccctgcatac ggcccaggtc
     1861 gtatcggcca tcaacccgac catgctcagc gccttgtcgc tcattattcc ccgcgaagta
     1921 ccgctctacc aggatgtcgt caatggaacg tttacccccc tgacagcgcg gggcttcctc
     1981 caggaactgg acatgatcct gcgccatacg gaaatgacca agccctgcat cttccgcagt
     2041 aaccacgttt ccaatctcct gcctgtcggc ggcaccctgc cgatggataa ggaaaaaatg
     2101 ctggccactt tggaacgcta catcccgcaa gtcgacgaca cgctgccgct cttaaacgat
     2161 aacggcaatt tctgattttt tgctggaggt gttgcctcgt gaaaaaactc tgtcttttgc
     2221 tcttcttctg catcctgtcg attacaacgg tctttgccga cggcccgcgc ttcaataaca
     2281 tgcgcactgt cgtcattgcc gatacgacac aggacagcta cgccgcccgt ttcatgaaaa
     2341 accggctgaa acagccgttc cgcatcccct actgggaccg catcgaaacg gatacagctc
     2401 tgtcgccgtc tgacgtcaac atcgacacga tgcgcgccct ttcggcccag tataaagccg
     2461 acgtcgtcct ggtccccgtc gtccagacct ggtactggcg gcagcacatg gccgggctct
     2521 ggcgctatga tgatgacgaa atcatcacgg aatgtctcta tcacctgacg gtatacgcct
     2581 acgataagcg gaccgatacc ttcaagagct acagcgacaa aggccgtgaa atcgaatcgg
     2641 catctatcct gaacgacccc aaggaaatcc tgacggaatc tatggaccgc attatgaaaa
     2701 agctgcccta taagcgcatc ccgacggata tcgaagacgt cgcaacggtt ggaacgtcct
     2761 tgcagacaaa aaccaccgaa ggcggggcta aaatactgac caacaccttc ccgcaggtca
     2821 tttgaggtta gggattagtg gctcgtggtt agtggctcgt aaaaaaaagg ggtgtcgcat
     2881 gtgggacagc ccctttttgt gttgtagggg cgacccgtgg ggcgcccgct ccggtatatg
     2941 tacgatgtcc caatcagctt gctaggaaaa ccatccaaat ccctagcggg tcgcccacgg
     3001 gcgaccccca caaactttca ttcatgcaaa cgacacacat tcttgtggcg ggctcgccac
     3061 gtgcgagccc ctacgttaga atgctacttt tttatgaact tttatctata aggtttccta
     3121 tttcatgcag gctgatttct gctttttggt catcgaaggg ttcgcagtgg atgcggttgc
     3181 ggccggcttc tttagcctga tagaggaaat ggtcggctaa ggcgaacatt ttccgcaggt
     3241 cgacagcgcc ctggcagagg ccccacgtat agccgccgct catcgtcggc tggcgggtca
     3301 aatcgtcaaa gggcaagtcc ttcaagtgcc gctggaaccg ctctacatcg tcctggaacg
     3361 aaggttgatt ccgaaatgac aaaatcagga attcatcgcc tccatagcgg tagacacaat
     3421 catccgggaa gatatggcac aaaagccggc tgatgccgga caggatatca tcgccgagta
     3481 catggccata agtatcattg aaaaatttaa accggtcgat gtcgcaaatc atgacacaaa
     3541 tcgtctgtcc taaatactgt tcaaaatctt cccgcagggc ttcgcggttc agcagcccgg
     3601 tcagctcatc ggtccggctc cgctggtaaa actctcttca ttactggacc ggaggtgcat
     3661 gcgtatctgg gcaaaagcca gggccagatg gcgccgcgaa aagatgatga ccagacaaac
     3721 cagataaaag aggacgatat cgtattggtc ctgccaggca atctgatgac ctgtcatggc
     3781 gatgacggca aaggtcgtga ccagattgac ggcgacgatg ggaaacaaaa ccttgaggtt
     3841 atcgtaaatg ggcagcaaat agaccatgat gagcatgagc aggtattcaa agaagaaact
     3901 gctcccgtcc tgggtgctga aaaaagtcag gcacgccagg gatgaattga tgaacaaaaa
     3961 gatatagacc agattttcta atatcttttc attgcggacc gctccgtgca gacatcggta
     4021 gaaaccggca aaaaagagga cattgacgct aaaaatgatt ccatagactg ccgttgcatt
     4081 gatagccggc aagacggctc cggctgcatt gggcgcatac gtaccgatga ccccggtcag
     4141 ggcgatgatg cacaggaggc aggaaatgaa actgcaggca tagagattat cccgtatttt
     4201 ccgtttcctg aattccggat ccgccaaaat agcctggata tcaccaaatg gcccatgaaa
     4261 tattttatgt acatgttttc gtaaatatat aacaaatctc atgaaaaagc ccccatttgt
     4321 ctatacttat agtatcaaaa caaatgaggg cttgcaagtt ttactcgatg acctcaatga
     4381 ctgcccggta cggcttccat gagcgtttcg gcctgagcct tcgctttttc tttggtttca
     4441 tgagcatcca tgtggcgggc aaagatgccg gccaggatag aacccgttac ctgatggata
     4501 gccgctccga cggctgccgg aatggcgacg gccggggcaa agaagaccga taccagcgaa
     4561 atggccaggg cgtcgttctg catgccgact tccaacgtga ccgagtgctg ctgtttcttg
     4621 cccaagccca gcttgcctgt aacgaaatag ccgatgagga agccgccgag attgtgcagg
     4681 aggaccatga ggatgatgaa gatacccatg tccaggattt tagcgccatt gatggcaatg
     4741 acgccgccga cgatgaccag gacgccgaaa gccgaaacga ggaccaggac cttcgacagg
     4801 gaggcaatat gtttttctcc catcaggcga tgcgtcaata agccgaggat caagggaacc
     4861 aggatgactt tcatgactgt catggccata gccataaagg aaacttcgac ccattggccg
     4921 gccaggcccc agacgataaa aggcatcata atcggcgcca gcaaggtcga acacgtcgta
     4981 atggaaacgg acaggggtac atcggccttg gccaggaagc ccatgacatt tgacgccgtc
     5041 ccgctgggac aggagccgag gaggatcatg ccgacggcga tttccggcgg catttcaaag
     5101 gccaggacca gggcataggc caagaggggc ataatcgtat agtgagccgt acagccgatg
     5161 aggacttcct tcggccgctg gacgatcaat ttgaaatccc gggcatggag cgtcataccc
     5221 ataccgaaca tgatgacgcc taacaagagg accgtatgag gaaccgccca gagaaaggca
     5281 ttgggcatga aataagccca gatgacgacg agaaagacga tatacgtcaa attcttccca
     5341 acaaaaccgc tcaactttcc aatgaaatcc atcatagtca cgcacatcct ttctaaaata
     5401 agacctgaca aatacaaata cgcaaacggt tttgatggag caaaaaaaaa ctttgcctct
     5461 aaaaagagac aaagtattca ctctgcggta ccactctcat tgtcgtaatg accacttttc
     5521 cagcatctcc gggacagccc atgtaatgcc tggcaaggta acggttgcct gccggctgcg
     5581 tctactagca aaatacgttc gggcagcagc tcctggacga cttcacgccg ggattccatc
     5641 ctgcctctca gccgccagca ggtctctgtc atggtttccc aaacattact atttccattc
     5701 aacactgttt tgtatttgtt gaggcctatt ataccgtgtc gctcccgtga agtcaataag
     5761 cttttaagtt agttgtacgt tttaccgtca aaaatttaca tatgtcatgg tcatcttttc
     5821 tatattttat agttgaaatt tgaatttgaa ttatatataa tagaaagaga aaattctgct
     5881 aatctgcata ccgtcctaag aaagaaggaa tgtccatgct ctccgcttcc ctgcaagccc
     5941 ataagaaaca gctcttcatt ttcatcatcc tcctggccgc tgccgctggg gcctggtgct
     6001 actggcagca gcctaaagca gcgccgctta cgctccatgg caatgtcgat gtccgtcagg
     6061 tttcgctcac attcaatgcc agtgaacgca tcgcctcgat gaatgtcgaa gaaggcgacc
     6121 gtgtccacca agggcaggtc ctggctacgc tcaatacgac gcccctaaaa ttgacgattg
     6181 cccgctgcca ggcgcaaatc gcccagcagg aagccgtcgt cgctaagatg aacaacggct
     6241 cgcggcccga agaaattgcc gaagcagcgg cttccgtcca atcggctcag gccgaagcgg
     6301 ataatgccgc cgccgattat cagcgcatga gtacgctcta tgccggcgac gccatcagca
     6361 agcagacctt ggatgcagcg gaagccaagg ccaaatcgac agcggctacc ctggcccatg
     6421 ctcagtcggc ccacgacctg gccagcaacg gctatcgcca ggaagacatc gacgccgcac
     6481 aggcacagct ccagtccctg cagaacgaac tgcagaccgc ccagtataac ctgagccagg
     6541 ccactctcgt cgccccgcag gacggcgtca tccgctcgcg cctcgtcgaa ccgggcgaca
     6601 tggcctcacc gtcgacaccg gtctatacgc tcagcctgga tacgaccaag tggatccggg
     6661 cctacatttc ggaagataaa ctgggaaaaa tccacgaagg ccagtcggca acggtcacta
     6721 tcgacagcgt cgacacgccc ttgacgggcc aggtcggtta catttccgac acggcagagt
     6781 ttacgccgaa atccgtggaa accgatgacc tcaggacatc cctcgtctat gaagtccgga
     6841 tttacgtcac cgaccccgac aacgtcctgc gcctgggcat gccggcgaca gtcaccttct
     6901 gaggcagaaa ggaaggattc ccatgacgaa ctgtcagcct gcatcgacct ccccagccct
     6961 cgaggcccag gggatcgtaa aacaattcat ccgccctggt cagtccagtc cggtcacggc
     7021 tttatcaggg gtttcactga ccgtccgcac cgggagtctg acggccatca tcggccctga
     7081 cggggctggc aagacgactt tcatgcgcct cgtctgcggc ctgttgaaaa gcgatgccgg
     7141 cagcctgtcg gtcctgggta tggacgtggc cagccatcct caggccgtcc aggaccatat
     7201 cggttatatg ccccaggaat tcggtctcta tgaagacctg tcggtccagg aaaacctgga
     7261 cctctacgcc aacctccacg cggtccctca agccgtgcgc caggagcgct tcaagaaact
     7321 gctcgacctg accggtctga gcccgttcac gaagcgcatg gccggcaagc tctctggcgg
     7381 tatgaagcag aaattggggc tggcctgcac cctcgtttcc atgccgccgc tgctgctcct
     7441 cgacgagccg acagtcggcg tcgaccccct atcgcggcgg gaactgtggc aaatcctcga
     7501 caccctgtcg gcatcggggt ccctgaccat cctggtcgca acggcctacc tggaagaagc
     7561 cgaacactgc cacgacgtat acatcctgca taaagggcat atcctggccc agggcgcacc
     7621 ggacacgctc tgccactatg cccagggccg ctgttatgtc gtcaggccgc cggaaaatgt
     7681 cccaccgcgg ctcatacagg ctgccctcat cgacgaaacg gaagccgtag ccgatgccgt
     7741 tcccgaaggc ggggccgtcc gtttcagtct gacgccccag gccagcctga aggacctcgc
     7801 cctgcccggc ctgacagtaa cggccgtccc ggccagactg gaagatagtt tcatgagcct
     7861 cctgcgccag caggaaccgt cccttccctc ccactacagc cgccccgata tgactgctgc
     7921 caaaagagta gaaacaggac ggaaaatcat cgaagtccgg gacattgtcc gccgcttcgg
     7981 cgatttcatc gccgtcaatc atacgtcgtt cagcgtctac gaaggggaaa tcttcggccn
     8041 nnnnnnnnnt acgccgggga attgttacgg tgtgatcggg gcgaacggag ccgggaaatc
     8101 gacgacgttc cgcatgctct gcggcctgct tcccgtatcg tccggccaga tttccatagc
     8161 cggcgtcaac ctgcgaaccg cccgagccca ggccagggcc catatcggct acgtagcgca
     8221 aaaattctcc ctctacacga cgctgtccgt cgaagagaac ctccgcttct tcggcagtgc
     8281 ctacggcctg cgcggccgcc ggctggacca gcgcatcgaa gcggtcatcg aagaattcga
     8341 tttgtccggc ttccgccata tgccggccgg cagccttcct ggcggctaca aacaacgcct
     8401 gtccatggca gcggccctgc tccacgaacc acctatcctc tttctcgacg aaccgacaag
     8461 cggcatcgac ccgctggccc gccgtctctt ctggcggcag atcacggacc tgtcggccaa
     8521 ggggacgacg atcatcgtca cgacacattt catggaagaa gccgaatact gcgaccgcat
     8581 catgatacag gaccgggggc agctcatcgc catcggcacg ccgtcggcca tccggaaaca
     8641 gggcggtaat agtccgacta tgaatgacgc cttcatccat atcgtcgagt ccggccgcca
     8701 tcatcaggag gtatcgtcat gaacctgcat cgttttctcg ccttatgtca taaagaattc
     8761 tgtcagatgc tgcgcgaccc cagcagcttc ctcatcggca tcgtcatccc tctcatgctc
     8821 atcatcctca tgggatccgg cctatccctc gatgtcaagg acctgcccgt cgccgtcgcc
     8881 ctggaagaca gctcgccgac ggcccgcagc acccttcact tcctcgacgg ctcgccatat
     8941 ttctcaccgc actacgtatc ttccataagt gaggccgccg ccctgatgga tgcccgccag
     9001 gccgatgcca tcatcgtcat cccgccggat ttcacggacc gcctggaaca aggccatgcg
     9061 acggtgcagg tcctcttgta tggcatcgat gcgacgacgg ccaatgcggc caatacctat
     9121 atttccagcg gcttccaggc ctggcaggcc gcttgggcca aaagccacgg cgcagcgtcc
     9181 cggacgggta cgattacact ggtgtcgcgg caatggttca acgacgccaa tacgagttca
     9241 tggatgttca tccccggact catcgtcatc gtcatgaccc tggtcggcgt cttcctgacc
     9301 tccctggtca tggcccggga atgggaacgg ggcacactgg aatccctctt cgtcacaccc
     9361 gtccggccgc tggaaatcat tgccgccaaa atgataccct atttctgcat cgccctggtc
     9421 ggccttgttc tctgcctcgt cgccgcttat tacggctacg gcgtgcccat gcacgggtct
     9481 ctggccttga ttcttctatc atctattgaa tacatcttcg tcgccatcgg catgggcctc
     9541 accatttcgt cgctgaccaa gaaccagttc ctggcctgcc agatggccct ggtcgtcagc
     9601 ctgctcccga cgatcatgct cagtggtttc ctctttgacc tgcgtagcgt accgacagcc
     9661 attgccgtca tcggccacgt cctgccagcg acgtattata tggaattgat gaaatccttg
     9721 ttcctggccg gcacgacggg gcccctggtc atcaaggact gtgccatcct cggcgcttat
     9781 gccgtattct tcgttttcct gtccctgcgc gtcaccaaaa agggggttgc ctgatggaca
     9841 cattccgact gactttttat aaaatctacc agctcatcgt caaagaactg ctgacgacct
     9901 tgaaagaccc gaagagccgt gtcatcctgg tcatccccgt cctcgtccaa ggactcctat
     9961 ttgggtatac ggcgacgtat aacctcgaca atgtaccgta tgccctcgtc gatgaagacc
    10021 acagccggga atcggccgat ttcatcgccc atctcgatgg gaccggcatc ttccagcgcg
    10081 tccagacctt gtcgtcgaca cagcagatcg cccaagccat cgacagcggc gaggccatga
    10141 tcgtcattac catcccatcg gactttgccc gccgactgaa cagcggccag gaagcgccga
    10201 tccagacggt caccgacgga cgcaatccca tgacagccgg cctggcggcc agttatgctg
    10261 cctctatcgt ctcagccatg aatgtccagc gaatcggcca tcagccactc atcgaaatcc
    10321 gttcgcgcat ctggtataac cccaataaca ttacacgccg catgttcctg ccatcgctca
    10381 tccccatgct ggccttgaca caggtcctca tcctggccgg cctctccgtc gccagggaac
    10441 gggaaaatgg caccttcgac cagcttctgg tcacgccgct gacgccgcca gaaatcctca
    10501 tcggcaaagc cgtgccgcct atcctcatcg gcctcgtcca ggcgacgctc atgttgtgca
    10561 tcgccgtgtt ttggtttaaa atacaattag tcggttccct ggccgctctc taccttacga
    10621 ttttcatttt cctcgtaagt tgtgtcggcc tgggcctgtc gatttcagcc gtagccaaga
    10681 acatgcagca agtcatggta tactgtttct tattcctcat gcccatgata ctcctgtcag
    10741 gactggcgac accagtccgc aacatgccgc aggtcctgca gtacgtgacc tatatcaatc
    10801 ccatgcgctt tgccatcgaa gccatacgcc gcatctacct ggaaggcgcc ggtatccctg
    10861 taattgccta tacttatctg cccatgctgg ccgttgccgc cgtcaccctg cccctggctg
    10921 cctggctgtt tcgccataaa ttatcataga aagagggatt gctatgcctc ataaagcgcc
    10981 tgtgacgtcg agccgcgaag acggccggga aacaaaagca cgcatcatcg aagctgcagg
    11041 cccgctcttt gcccaacacg gctacgacgg tacgacaagt aaggaaatct gccaaaaagc
    11101 tggtgtcaat atggcggctg tcaactatca cttcggcagc cgcgacggcc tgtacgtcgc
    11161 cgtcctcgaa gaagtccagg attacctggt caatgtggac gtactcaaaa aactggcgca
    11221 gcaagaaggc tcgccgcgcc agaaattaga acgttttgtc gattatttta tagaagatgc
    11281 ctttcgccgc aacgactggc acgtccgcgt ctggatccag gaactcctgc acccgtcgcc
    11341 gtacattcag aatatcattt cccaaaaggg cctgcccaaa ttctacgtat cctcaggcgt
    11401 cctgcagcag gccttgggct atgacacggc ggatatcgga ttttacagtg cctacctcag
    11461 cctcatcgcg ccgttcaccc tggccttcct ggcccagaac agcccggtca tcaaggctct
    11521 gcccgtccac tatccaaagg aagaattcat ccagcactta aagaaaaact tcttcaagac
    11581 tctcgatgcc ttgacggttc ccaaaacgta gtgcctggct gatttacggg agtacagacc
    11641 ggacccgatt ctggagctcc ggcacgacgt cggcctcgaa ccagggattc ttggcctgcc
    11701 agcggaagtt gagcggactg ggatgaacga tggggaaata gtccggcaag taagcctgat
    11761 aattccgcac agtctccgtc aggttgcgtt ccgtccggcc tttcaaataa tagtcgatgg
    11821 cgtacttgcc gacgaggatg gtcagtgcca ggtccggcat gagttgcaga atttcacgat
    11881 gatattcctg ggcgatgaac cgtctcggcg gtttgtcgcc cgttttggct ttgcccggat
    11941 agtagaaatc cataggcata atggaaatgg ccggcgaata gaaagtctgg ctgtcaatgc
    12001 ccatccaggc catcagcttt tcaccggact tgtcatggaa aggaatcccc gattcttcga
    12061 ccttcttgcc cggagcctga ccgatgatca agattctcgc tttctcgtgg atctgaaaaa
    12121 taggaccgat gccgcgggcc gtatacgacg cgttccgttc gtccgttttt aattgatcaa
    12181 tgatctcctg caaatgcata aaaacgcctc ctaaatggta aagagcctgc catgatacac
    12241 gaataagctg tggtcgaaca tcaccacggg cctccctggc cgggacggcc cctacgtagg
    12301 cggcagatag ccgcgttatt tttcatcgcc tgtatgcgat gccgtagggg acgcctgtct
    12361 aaggcgtccc gccgtgaatc ctgtaaatga ttggaatata tgacaagtgg cacattgtcg
    12421 taataccatc atcagacgat taaatgataa taaataatag gtatgaatag ggctggcagg
    12481 aaatcggcta ctttcagctt cgtcaactgg aacatgttga gggcaatacc caggatcatg
    12541 acactgccga cggcattcaa ttcccggatg acatcgggcg tcatgagggg catgatgaag
    12601 gtcgccaaca gatagaaaaa gccctgatag atgaagagga cgatgccgac gggaacgacg
    12661 cccaagccgt aaagggtccc aaaaatgaaa gaagacgtaa agtccagggc gctcttgaaa
    12721 tagagaaggt catgactccc cagggccgcc tggatagggc cgaggatggc catggcgccg
    12781 atgacctgca tgatggataa ggtgacgaag ccccggacga accgcgaatc atcgccgtgg
    12841 cccatgcgtt tcttgaggaa ttcgccgaag cggttcatgc catcttcgat gccgatagcc
    12901 gtcccgacga gggtaccgat aatcatgcta gccaatacga ggagcagatt ctggctctgg
    12961 acggctcctt gtatgcccat gatggcgacg cacagagaaa tgacaccaaa aatggccttg
    13021 gtaaagcgct ccggtatgcc gcgcttcaaa agaaatccca gcaatgcccc taatgacgac
    13081 gtcacggcat tgacgatgac agcgaacatg tactttcccc ctgacaagtg aatttgtatc
    13141 cattatagca gaaaaaagga gctgtcgcac gaagacagcg ccttttttta gcagttagca
    13201 gctagccgtt agcagttagt ggatggcttt gaatttaaag gctttctatg taaactacaa
    13261 actccatact ataaactata aagagaatgg gagcgcacat gatggcagaa gccttcgtgc
    13321 gatagtctct tttagcagtt agtggatgga atcgaattta atgggctgct gtgggcgata
    13381 cgctggcagc ggctttctgg tcggtttcgt agttggagtc gaagcgtttg ttcaggtagt
    13441 tgacgtagag ataggccaag acggcaccgg agatgttgtg ccatgcactg aagacggcac
    13501 cggggacgcc ggccatgggc agggatgcaa agtgtgcttt ggccaggccc gttgctaagc
    13561 cggaattctg catgccgact tcgatggaca aggcgaccat tttcttccag ctcatgccgc
    13621 agagatggcc gataaagaat cccagggcat agcccaggca gttgtgcagg atgacgacca
    13681 gcaggatgat gcccgaactg ttgaggatag catcgcggct ggctccgatg atgccggcga
    13741 tgaggaagga aatagccagg gccgaaacag ccggcaggta atccgcagcc gtatcagcca
    13801 gcttcggcag gaagctcttg acgatgaggc ccagagcgat ggggacgatg acgatctgca
    13861 ggatcgataa gaacatgccg accgggtcaa aggagatgcg ctgaccgatg atgagatacg
    13921 tcaaaagcgg cgtcatgatc ggcgaaatta acgtcgatac actggtcatc gtgaccgaca
    13981 ggggtacgtc gcctttgctc atgaaggtga tgacattaga cgatgtaccg cccgggcagg
    14041 tcccgacgag gacgacgcca accgtcaggg ccgggtcgag ctggaaaatc gtcgaaagga
    14101 cgtaggccag gaaaggcatg acgaaatact gggcgcaggc gccaaaaaag acatctttcg
    14161 gccgcttgag gaccagggcg aaatcgtgga cgctcatggt catgcccatg ccgaacatga
    14221 tgacgccgag gaggacggag agaacggaaa tgccgcccac cttgccgagg acccaagaaa
    14281 agctcgtcgg cagggtcatg ccgatgatga ggaagatgac ggcgatgacg ccgaaatatt
    14341 taccgataag ggcacagact tttttcatga tgaatacctc tttttaaaac aatatgttta
    14401 taagtccaaa aaaaaggaac ctcctgcccc gatggggatt ctccgccatt ccgaagaggc
    14461 ccggccaggg caagaggctc gttgaaagac taattatttc atgacagcgc cggtattggc
    14521 agacgtgacg agtttcgcat agcgggacaa gtaaccggtc ttgaccttcg gttccggctg
    14581 cttccattca gctttgcgtt ttgccaattc ttcatcgctg acttccagtt ccaatttacg
    14641 gttgggaatg tcgatggaaa tagtgtctcc gtcgtggacc agggccaggg nnnnnnnnnn
    14701 ggggccgcct tccatagctt ccggcgagat gtggccgatg caggcgccgc ggctggcacc
    14761 gctgaagcgg ccgtcggtca agagggcgac tttcaggccc atgccggtga tgacggctgt
    14821 cgggttgagc atttcacgca tgcccggacc gcctttcggg ccttcgtagc ggatgacgac
    14881 gacgtggcct tctttgattt caccgccgat gatagcggca atggcagctt cttccgagtc
    14941 atagcatttg gccgtacctt tatagaccag gaggtccggt tcgacggcac tttccttgat
    15001 gaccgagccg agaggcgcca ggttgccttt gaggacggca atgccgcctt tatgcatata
    15061 ggcgttttct acggaatgga tgacgtcgtg gttgaggatc ttcgcatctt tgatgcggtc
    15121 gccgaccgtg ccggtaatgg tcttggcgtc ggtgtggatg aggcccaact tggagacctc
    15181 tttcatgaca gcgcagatgc cgccggcttc atcgaggtcc tgcatatgat acgtgccgcc
    15241 agggctcatc ttgcagatat aggccgcttt acggctgatt tcgtcgaatt catcgagcga
    15301 gaggtcgacg ccggcttcgt gggcgatggc cggtaaatgg agggccgtgt tagtcgaacc
    15361 accgatagcc atgtcgacgg caatggcgtt ttcaaaggct tccagggtca tgatgtcgcg
    15421 cggcttgata tctttgcgga ccaaatccat gacgacctgg ccggctttct tagccaacat
    15481 gcggcgcagg cccgtatagg cagcgggaat ggtgccgttg ccgggaaggc ccatgccgag
    15541 gacttcggtc atgcagttca tggtgttggc tgtgaacaag ccggaacagg aaccgcagcc
    15601 cgggcaggcc tggaattcca tctggtgcat ttcttccggt gtcatctggc ccgattcgac
    15661 tttgccagcg gcttcaaaca tggtgctgac gctgatgttc ttgccgttgt gacggccggc
    15721 gagcatgggg ccgccgctga cgacgactgt cgggatgttg aggcggccgg cagccatgag
    15781 catagccggg acgattttat cgcagttcgg gatgagtacc aggccatcaa aggcatgagc
    15841 tgtcgctacg gcttcaatgg agtcacagat cagttcacgg ctggacaagg aaaagctcat
    15901 gccgcgatga cccatggcga taccgtcgca gacgccgatg gacgggaatt caatcggtgt
    15961 accgccggca gcggcaacgc cgagcttgac tgcttcggca atatcgcgca aatggatatg
    16021 gccgggaatg atttcattga aggcattaca gataccgatc aacggtttct gcaaatcttc
    16081 atcgctatag cccatagcat ggaataacgt acgatgggcg gttcgggtaa cgcccttttt
    16141 aacaacatca ctgcgcatgg tattcatcct ttctctacat atccttgacg gccatttcgc
    16201 cgcgggacag ggaaatctgc ccggtccggg cgatttcaat aatatcataa tctttcagca
    16261 tttccaaaag agcctcgata tggtcttccc ggcctgtcag gcgcaggacc agggaacggc
    16321 gctggacgtc gacgatcttg gcgttgaaga gctgggcgat attggtcagt tcttcgcggg
    16381 tctgcggcgt cgggctctgg actttgatca tcgccagttc ataactgatg aacggcgcat
    16441 cgtcgaggtt gacgactttg atgacgtcga tgagcttggc cagctggttg acggcctggt
    16501 cgagttccca gcggttttca acggaaacga cgatgttgat acgcgttacg tcagcttctt
    16561 ccgtataacc ggcattgata cattcaatat taatggctct gcggctaatc aggccggcga
    16621 cgtgcgtcag gacgcccggt ttattgtcgc agagaatagc gagatgttgt ctcatcattt
    16681 cccttcacct cccaatacca tctggtccag gcgcttgccg ccgggaacca tgggatagac
    16741 attttcatca gccgggatga agacgtcgag cagtgccggc ccttccgtct tgaacagggc
    16801 ttctacggct ttagccaggt cttccggctt atggacggcc gtcccgggaa cccccatgga
    16861 cgcggatacg gctgtcaaat cggggttatc cccaatcagc gattcagcgc agtggccgtt
    16921 gtagaacaag cgctgccact ggttgaccat gcccaggacg ttgttgtgga gaacgatggt
    16981 cttgacatcg acgccatatt tatgaagagt ggcgaattcc tggcagttca tcatgatact
    17041 gccgtcgcca gtgaataaga cgactttcga cttgggacag cccagcttgg caccgagggc
    17101 agccggcagg ccatagccca tagtacccag accgccagag gtaacgaagc ggcggggctt
    17161 atttaaatcg aagaactgag ctgcccacat ctggtgctgg ccgacgtcgg taacgacgat
    17221 ggtgtcgtcg tcggtccact tctggacctc ttcaaagagc ttctgcggcg aaacggcatc
    17281 gggaaggtcc cgcgtggcaa agggatgagc ggccttcata tcgaggacga aagtccgcca
    17341 ttcaccatag cggtccgcca gatgcgtttc atgagctgcc agcttttcgt cgaggagcgg
    17401 cagggaccag cggaggtcgc cgttgacgct gatgtcggcg tggacgattt tattgatttc
    17461 tactttatcg atatcgaagt ggacgatctg cgcgttcggc gcgaaatttt cgacgtcgcc
    17521 agtgacgcgg tcatcgaatc gggcgccgat ggcgaccagg aggtcgcatt tcgtaacggc
    17581 catattggca gcgtacgagc catgcatgcc gaccatgccc aggtgggcgt catacgtatc
    17641 ggggacgcag cccttgccca tgagggtcgt gacgacaggg acatgcatct gctggacgat
    17701 gtgtttcaag cggtccgaca tgttggaaag gacgacgccg ccaccggtga ggatgaccgg
    17761 gcgctttgcc ttttcaatgg cttcgacggc gcggtcgaca tcggcttcat tgccgacgtg
    17821 atggccctgg tagccgcgca gatggacttc ttcgggatat tcaaattcaa aaggtgtatc
    17881 gaatacatcc ttggcaatat cgacaaggac cggcccgggc cggcctgtgg ttgcgatgta
    17941 aaaggcttct ttcaagaccc ggggcaattc cttgacgttc ttcactaaat agttgtgttt
    18001 cgtaatcggc gtggtaatac cggagatatc agcttcctgg aaggaatcgc gcccgataag
    18061 gggcgttgct acctggccgc tgatgacgac gaggggaatg gaatccatgt tggccgtggc
    18121 aatgcctgta accatgttgc agacgccagg accggacgta gcaaagcaga caccgggctt
    18181 gcccgtagcg cgggcatagc cgtcagcggc gtggacagct ccctgttcgt ggcctgtcag
    18241 gacatgggga aatttggctt tatataattc atcatataat gtcaggacag ccccgccggg
    18301 atagccgaag acgacttgta cctgttcacg tttcaagcat tcgatcactg cttgtgctcc
    18361 ggtcattttc atctcattca cctcatcata aaacatgact catacttaca aagaagctat
    18421 tcttcaatca acctgcttct tgggtttgat gtctgaagtt tgatgttaaa agttatttcc
    18481 aacttcaaac tccaaacatc caacttcaaa ctatgtctta gtcgttctgg tcgttgttgt
    18541 ggagccacgg catcatggca cggagttttt caccgacctg ttcgagcgga tgttccgcat
    18601 gctgacggcg catagccagg aagtgagcgc ggccgccggc tttgttttcg aggagccaat
    18661 ctttggcgaa ggcaccggac tggatgtcgt
//
LOCUS       DAWABZ010000021        18330 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_829_length_18330_cov_3.614993, whole genome shotgun sequence.
ACCESSION   DAWABZ010000021 DAWABZ010000000
VERSION     DAWABZ010000021.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 18330)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18330)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18330
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_829_length_18330_cov_3.614993"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    9327..9336
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13135..13144
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4305 a         4864 c         4990 g         4151 t
ORIGIN      
        1 ttccaggcag accacggact ggattgtgat ggcagcagat ggctttaaat ttaaaggttt
       61 tcgctaaaaa ctacaaactc cgtactacaa gctataaagt gaaaggaatc tcgcatgatg
      121 gtataagcct tcgtgcgaca atggccccat actataaatg tggcgaaaaa aatggtataa
      181 ttaattatct atgttacaaa tttgggaaag gggtattcca tggatacgac aaagaagata
      241 gccgttatgc tgggggtgtg cttcatgacg tccctgtcgg tggctggtgc gacgttccat
      301 cccggtgaca agggacatca gattactgaa atccagcagg ctttgacggc gcatggttat
      361 gatacggatg cggatggcga ctatggcgat tcgacccagg ccgcagtccg tcagttccag
      421 gcagaccacg gactggattg tgatggcatc atcggcgccg ctacgtatga agccctcatg
      481 ggggaagcca tgccggaaaa caggacttcg cagggagggg cggcttcgga cgctttgccg
      541 gctgttacgg cagacaatga agtccgcgtg atccagcagg cactggccaa taatggttat
      601 accgtcgatg tcgatggcgt ttttggcgtc gggacggagc aggccatccg ccagttccag
      661 gccgaccatg ggctggaaac ggacggcatc gtcggccagt cgacttttta tgcccttacc
      721 gggcagggcc tgcccaacgg tcctatccgc cgtttcggca atggcggata taatggcctg
      781 gcccgcgtcg aaagtccggc ggctacgcat atcctcgaca ttgccaacca gtatatcggc
      841 gttccctata tctttggcgg ttcgacgccg tccggcttcg actgctccgg cttcacgcgc
      901 tacgtctatt cggcagccgg catcgacttg ccgcgcagtg ccgacgaaca gtatggcgta
      961 ggttattcca tttccatggc taacctgcag cctggcgacc tggtcttctt ctcgacgtac
     1021 gactcgggca tttcccacgt cggcatctat atcggcaatc accagttcat caatgccgcc
     1081 agcgatggcg tcagcatctc cgatatggac agcagttatt gggctgcccg ctacatcgga
     1141 gccaaacgcg tcatgtaatg tcagaaaaaa tcctacagaa aaatcagaat ttttcggcta
     1201 ccgtcagcaa aatccgttcc ttataataga aaccgtaaat tgcggaatta tgattacgga
     1261 tttttgcaac ggaaggagtt tttcactatg ttcaacctga tttggaattt tttcaagacc
     1321 gctccggatg ctccgcagat gacggatgcc gcggcgatta agaagaaatt caatgccatg
     1381 cgctggcgcg tctttgcgtc cattaccatc ggctatgcct tttactacat catccgccag
     1441 agttactcgg tcatcaagaa gccgctcctg gcttcgggga tcgtcacgcc gacggaaatc
     1501 ggtatcatcg gttctatttt ctatgtgacc tacggtcttg ggaagttcac caacagcttc
     1561 ctggcagacc ggatgaacaa taaacggttt ttctcattcg gcctgttcat ttcctcactt
     1621 accatggtcg gcatggggct ggtcaattcc tttgtccccc tggccgtctt gtggggcttt
     1681 aatggctggt tccagtcgta tggtgccggg ccgtctatcg tttcccttaa ccagtggttc
     1741 agcaataaac aacgcgggac cttatatggc atctggttca cgagccacaa tctcggctct
     1801 tctttcgctt actttgccat tgctgccatc gtctccatgt ataactggtc tatgggattc
     1861 atttcggccg gcatcatttc cctggtcggt acggtcttca tctactttgg catgagtgac
     1921 cgcccggaaa cgcagggcct gccgaatggg gctgtctact atggggaaaa gacacaggaa
     1981 cagatcgacg aagaaaagaa aaaatccgtc ggtgccatgc agtggaatgc tgtcgtcaag
     2041 aatccggccg tctggatcct cggcttgtcc tcgctctgcg tctatattgc ccgctatgcc
     2101 atcgaaagct ggggcgtcgt ctatctgacc tcgcagaagg gctatgacat catgggcgct
     2161 gccggcgtca tggcttacat gcaggtagcc ggtatcttcg gggctctgtt gtgcggcgtc
     2221 atttctgaca aattctttaa ccacaagcgc aacatgccgg ccttgctgta cgggattttc
     2281 tactccttgt cgattgccgg tttcctctgg gcaccgcagt cgttcaccat ggatatgctg
     2341 tgcatgacct tctacggctt cacgatgggc gccctggtct gctacatggg cggcctcatg
     2401 gctgtcgata tcgtaccgaa gcgcgtcacc ggggcagcca tgggcatgat cggcctgctc
     2461 agttatgccg gtgcagctat ccaggaattc atcactggga agctcatgac gattaccgtc
     2521 gtcaacggcg tcaagaccta tgatttcggc atggctacgg aattctgggt cggtgccgcc
     2581 atcgcttcga ccgtattggc cctcctcgta tggaacgcca aaccgaaaga agattgattc
     2641 gttaatctga aagaggttgt cttaatgtga caacctcttt ttgcgcatct ccatcctgta
     2701 tgatatagta aacgtaatga tttatggagg taacagatga aacgggtcct ttcactgatc
     2761 gtgctggtca tgctttcttt gttctgcgca gcctgtgccc gtccgcaggg ctatatcgac
     2821 ttcacccagc ctcaggatac gcatccggct gcagaggaac cggatacgaa gccgctgcgc
     2881 atcgcctttg cgtcggtcat gtcgccgaag gaaacgcggc aggtatacca gcagatcgtc
     2941 aattatattt cgcagcagga aaaccggccg gccatcctca tccagcgccg gacctatgaa
     3001 gaactgaata cgctgctggc caatggcgat gccgacgtgg ccttttcttc gactggggcc
     3061 tatgcggctt atcaaggtca tgagcccatt gaactgctgg ccatgaccga aacgaatggc
     3121 tcgtcgtatt atcagacgta catcattacg gccgcagaca gcgacatcac cgataaacac
     3181 cagctccagg ggcgggtctt tgcttttacg gaccccttga gctattctgg ctgcctggcc
     3241 attactttcc gcctggccca gcaggggacg acgccagagc agttctttaa gcgcttcttc
     3301 ttcactcacg gccatgacaa gtccatctgg gccgtggcca atcacttggc cgacgcggct
     3361 ggcgtggaca gccagattta tgagctcata gagcagacga atccccagct ggcccggcag
     3421 gtgcgggtgc tggaaaagat gccggaagcg ccgacagggc ctatcgtcgt gcgcagcagc
     3481 ttggactcgg aagaaaaggc gtccttgcgg cggatattct atacgatgca cgaaaacccc
     3541 gatttatcgc ggtccatgaa gactattgtc atcgaccgct tcgtagctcc cgatacggcg
     3601 gcttatgagc agttcaagcg gcagtatgcg gaacagagac gattggcagg gacttgatat
     3661 gagaaactat acactctatg taaaatttaa cgccctcatc gtcggcacaa tctttgtctg
     3721 cggcctgctg gctgcttccc tgttccttta ctcgacgagc acctatatga attacgaact
     3781 cgatacatcc gggcgggaac tggcggcctc aatcagtcag gtcatcagta acgatatcct
     3841 cgtcgatgac cgctttgcca tgacggaaca gctgctccat gctcagcaga cgaatgacca
     3901 ggtgcggtat atcctggtga cttatcccga tggccgtatc ctggccagca cgtttacgga
     3961 cggcctgcca aagggcttgc cggaacggcg cgacgtgcag cacgacagag ccgataagac
     4021 tgtgaccttc gacagtacgg aaggatatat acgggaagtc gtctatccca ttgatggcgg
     4081 cctggtcggc tatttccgca tcggcttgtc ggaacggccg atgatgcagc tcatgtggaa
     4141 gcgcttcgtc cagaccatgc tggtcatcct ggccatctgt ctggcagctt ccgtactggc
     4201 tacgcgctat gcccgggctt ttttgcggcc tgtccagacc ctggcccagg ccatccgcca
     4261 aatcggccgc gggaattata gtgtccgcgt gcccgtcgag acgaaagatg aagtcgggcg
     4321 cctggcgaaa gccttcaatg tcatgagcaa ccgcctggct gtaaaagacc gggagaactc
     4381 gaaattgctg accgccctgc gggaaaagga gaagacccgt atctggctca tcgaccagct
     4441 cttttctgcc cgtgaagacg aacggcgccg catttcgcgg gaactgcacg atgagacgag
     4501 ccagtccatg gtatcgatgc tggcctattt gcggctcatc atggaccaga cagaagagcc
     4561 aaaggtccgg gaactcgtcg ccggtgtccg cgatttgacc cgcgagactt tggaaggcct
     4621 gcgccatctc gccgtcaatc tccatccgcc cctgctggat gacctgggcc tggtcgtagc
     4681 cattgaaaag tacctggaca cgtatcgcca gacacagccg gggctgaccg tgatcttcca
     4741 tcatgacggc gacatatcgc gcgtgtcccg gccagtggcc ttgttttgct atcgcatggt
     4801 ccaggagggg ctgaccaaca ttgcccgtca tgcccaggcc cataccgtga ccatcgggct
     4861 ccacgtcctg ccgtcgaagc tgaccttgtc tgtccgcgac gatggcatcg gcttcagtgc
     4921 ggaccgggcc gaacgggctc gcctggataa ccatttagga ctggtcagta tgcgcgagcg
     4981 gacagatgta ttgaacggga cctttgccat cgacagccat cctggtaaag ggacgacgct
     5041 gaccatttct ctaccgttgt atttgagtga ggaggaagaa catgaacatt ctcttggctg
     5101 atgaccaccg tatcttgcgg acggggttga aactattgct ggcctctcag gacgactgcc
     5161 atgtcgtcgc cgaagcctcg aacggacagg aagtcctgga gcggctggaa gacacgcctg
     5221 tcgacgtcgt cctgctggac ctttccatgc cggtcatgaa tggcatcgac tgtctgcggg
     5281 aaatccggcg ccgccattat gatgtcaaag tcctggtcct gaccatgtac agtgagcagc
     5341 agtacgtcac ggaactcatg cagctcggtg cagacggcta tctctgcaag gacacgctcg
     5401 atgcggaact tttcaaaggg ttgcgctctg tcgtttccgg acaccgctac ctgagtgaac
     5461 gcgaagcccg gagcctcctc gatggcgtcc tcgatggtgc cggccagcat ccggccctgt
     5521 cggcgcggga gcaggaagtc ctcgtggccc tggtccacgg ccattccctg tcggccattg
     5581 gcgacacact gcacctctcg gtcaagaccg tgtcgactta taagacgaga ctcatgcaga
     5641 aattgaactg caagaccaaa gccgaactcg tagactacgc cttgaaacac catttaatgt
     5701 gatggctttt aaatttgaaa ccatccactg actgctaaca gccagctaca acgggccgcc
     5761 ctttgggacg gcccctacga aaaaaaggct tgttgcatgt gcgacaagcc tttttttaaa
     5821 atgttttctc aataatcgcg atatcttcat cagtgacggt caggtccggt ccttggcgca
     5881 gggcttcgat ggcctgggta tcgacgaccc agagggccgg tgctagttcg gcggcgtggt
     5941 ctttcatcca cgttgcgatg gcccgggcgc cctggctata gggcaaggcg gctttgacgt
     6001 agcggaagtg ggagcgggga acggctttca tcggctgttt ccatacttct tcgatttcca
     6061 gggtatagtc acattcgtga tagccgttgg atacggcttc gcggccgatg tcttccgtgt
     6121 tcttctggcc catgtcgagg agcaggtagt cgtagagcag gacttgttcc cattttgcct
     6181 taaagatatc ccgttcaggg agaatctggc acggatcttc gctgtacagg cgcagcaggt
     6241 ccaggacgaa gcagaagcgg tggcgcttga aggtgaagtt cacggcgtcg acatagattt
     6301 cccgcatgcg gatatactgt ttcgtctgtt tgcggaaatc gctgtcaaaa tagctgatcg
     6361 gcgtctcgtg gcggatatac tcgaacagtt tctcttccag ggccatcccc tttttaaggg
     6421 ctgcccggcg gtcggccggg gctgccaggg gattgcggaa ggtacggttc agccggtcca
     6481 gatagttcag gcaggccgtt tccagggcca gggcgctgaa gggaccgtta tcatgctgat
     6541 gtgaaaaaat ttcgtctttt tctgctgccg atagcaatga tgtggacatc gtgatctact
     6601 cctctgaaag gtaaggatgt tcggctgcgg cactctggat ggccgcgttg gcatccttga
     6661 aataatcttt aaacgactgt acccatgtcc gggatgggtg cgataaatag cccttgcttt
     6721 tccagatcag ggccagggtc caatagagat ggggctggac gagggggacg gagacggcaa
     6781 ggtccggtga aatccgctga cagatcgtgt gcggcattag ggaaatcccc aggcggccgg
     6841 caaccatctg ggccaggaag tcccagttgc tggaacggca gacgatcttc ggatgggcca
     6901 tgatttcctg gaaagccgaa cgcaggtagg gattcaggga gaatccgctt gggtagaaga
     6961 cgaacgtttc gtctttgata tccgacaagg tcagcgatgg ctgggaggcc agggggtgat
     7021 ccggccagag aacgacgtcg agaggttctt cgtggaagat gaaaaattca taaggaattt
     7081 ctgtcgtaat cggcagggcg acgaacccga cctggatgag gccgtcatca atggcgaata
     7141 cgacgtcttg ggaaccgact tccttcattt ccagttcgat tttgggatat ttgttgatga
     7201 aatgggcgat gaacggcgcg atgacggaag agccgatcat aggcggtacg ccgacggaca
     7261 atttaccggc attgagcagc tgtggcgact ccatttcttc attcagctgg gtgaatccct
     7321 tgatgagttc ttcgatttta ggaagcaaat aggaacccgt ttccgtaagc tctacatttt
     7381 tccccttgcg gtcgaagagc tggacacccc agtgttcttc cagggctttg atccccttgc
     7441 tgatcgacgg ctggctgatg tgcagcgatt ttgaagcttt ggtaaagctt tttttgtgag
     7501 cgacttcgat gaagtatgtc agttggaata aatccatgat ataatgtttc acctctcttt
     7561 agtattctta tagtttacca tattacggaa tgaaaatcca atgatgtata cttctttata
     7621 aatggcggta ctaagccatt ttaaagcaat ctgtgtttct gtagactgtc taattgttaa
     7681 caataatttg tacgaattaa tgcgcccctc tatatttagg gcggccaatc gttgtataat
     7741 ataaagtaga atggaggagg gatggttatg gcacggcgat ggatcatgca tgtcgatatg
     7801 gatgcgtttt atgcttctgt agaacaacgg gataatccgg ccctgaaggg cctgccggtc
     7861 atcgtcggcg gcctgatgga gcggggcgtc gtcgcgacgg cgtcttatga agccagggct
     7921 tatggcgtcc attcggccat gagcatgaaa ctggcccgca ggctctgtcc gaatggcgtc
     7981 ttcctgcctg tgcgcatgtc ccattaccgg cgtatttccc atcagatacg cctgatcctg
     8041 tcccattact cgcccttcat cgaaccgctg gccctggatg aggccttcct cgacgtctcc
     8101 ggcatggagc gccattatcc ggatatcgtc gacatgggta aagccgtaaa ggaagaaata
     8161 cggcggacga cgggcctcgt cgcatcggct gggatagcac cgaataagtt cctggccaaa
     8221 ctggcatcgg accttcgcaa accggatggc ctggtctgga ttccctatgg gactgagcgg
     8281 cagatattgg cacccttgcc gctgagccgc ctgtggggcg tcggcaaggt gaccgaagag
     8341 gccctgcaaa aagcgggcta tcatactatc ggcgacatcg ccgcggccag tcccgaaacg
     8401 ctccagccca tcgtaggcaa tcaggcccag cggatttacc gcttgtccct gggccaggat
     8461 aaccggccgc tggaagtacg gcggcggccc cagtctgtcg gcaatgagca tacgtacagc
     8521 catgacctga cgcgcgacgc cgaagtggat gaacagttcc gcctcctggc caacgaagtg
     8581 tcgtggcggc tgcggcagaa ccggctcatg ggacggatca tcaccatcaa gatacgctat
     8641 acctctttca agaccattac ccgctctttg acgctgcaca gcatagggac ctgttcggaa
     8701 gaacagttat atttttcggc aaaaaaattg tataataaga gcagaggtca tgagcccatc
     8761 cgcctcctgg gcctgacggt cagccagctc caggcctatc aggtgcaggg cgacttgttc
     8821 tcggaagatg aagagacgaa ggcgaaagtg acggaagcca tcgacaagct gcagaaacgc
     8881 tttggccgca cggccatcat gaaaggattt ctttgggaaa tgtcccatga aaaagataca
     8941 taagaacgat aaagaaggag tttttgttat ggcaattttt aaagtagctg cccacacggg
     9001 tgataacaac aacgggtaca tcgaatacga tacagagacg aaagaactcg gcgtccactt
     9061 gaacgacgac gtcatgaatg aacgggcccg ccagtatttg acgacagaaa aagtcttgca
     9121 ccagtatacg gatttagcgc agtataagac catcagcacc gtcccgacat cgagcctgga
     9181 aatgctgaaa ctggccttgt gctatatctg gcgggctacg ggcatccaca ttgactggag
     9241 ccgccctgtc gattaaggga taagcctcca ccataatcta tgaacaattt atccacattt
     9301 catgtgcaca agggtgacag aaatgtnnnn nnnnnntttt tcaggtgcat actttttggg
     9361 gattcgcagg ggaaatgtgg ataactttcg tggataaatg tgtacctccg gtgaaaacgg
     9421 cctgaaaacg ggatgattac gcgatttata atggacgaac aaatggcgca gataatgtgc
     9481 ataattcctg agaaaatgtg gataaatgtt ctcggcaagt tatgcacaat atgtggatac
     9541 attgtggaaa ggtatacaca ggtggacaaa gcataaaata gccattctga cagtgtgtac
     9601 acaacttatc cacttcattt atccacaatt tccacagcct tgccgtttgt ggtatactgg
     9661 aggcaatgaa cacaattagg aggaaatcaa catgaaagct atcatcgacg gactcattgt
     9721 cctgccgaac gaaatcatta acggtcacgt cattatgtat gaaaaggata tctggcgcat
     9781 cgtaccgcgc aagcagttcc gggccggcat gtgctcggaa ttcattgatg ccaatggcgg
     9841 cttcgtcgta ccgggattca tcaatgaaca catccacggc tgtgccggtg ccgacactat
     9901 ggatgacgat gaccaggccc tgaagaccat gcagcaggcc ctgccggcga cgggcgttac
     9961 gtccttcgta ccgacgacga tgactatgga acagggacgc atcgagaaag ccctcagccg
    10021 gatccgtcag gcccgggacg actatcaggg cggcgcccag gtattgggcg ctcatatgga
    10081 agggcctttc atcagcccgg cctacaaagg ctcccaggcg gaagaaaata tccagactgc
    10141 cgatttttcc tggctcgaac catatgccga cgtcatcaag atcatcacct tggcaccgga
    10201 aacattgaag aacaagaaat tcctgaagga ctgccaggac cacggtatta tcgtgtccat
    10261 cgggcacacg gcggccgatt tcgaccaggc catggactgc atggacgccc tcggcagctg
    10321 ccatgttacg catttatata atgctatgac gccgttccac catcgcagac ccggtgtcgt
    10381 cggcgcggcc ctgctccata agggcgcctg ctgtgaattg atctgcgacg atttgcacgt
    10441 ccatccggca gcgcagaaac tggcctatca gatgaaaggc cgcgacggcc tcatcctggt
    10501 caccgactcg ttgcgggcct gcctgcaggg cgatggcgaa tccagcttgg gtggccagaa
    10561 agtcttcgtc aagaatggtg aagcccgcct ggctgacggg accctggctg ccagtattgc
    10621 ccccatgaac gaagtggtcc tccatttcct catcaattcc ggcgcctcca ttcccgatgt
    10681 cgtcgccatg gctacggtca acccggccaa agccctcggt gtctacgacc gcatcggttc
    10741 tctggaagaa gggaagcagg cggacattac catcctggac agcaacgatt tccatgtaaa
    10801 acagacggtc atcgccgggg agctggcata taatgaagaa gcctaatacg aagagcggca
    10861 ttttcctggc actggctatc ctggtgctgg cagccatcgg cgcctggttc ttctgggacc
    10921 gttcgccgtc gccagcgggc ccggcagggc cctctaaagg cgctgttcag gaagatgaag
    10981 catcgcggct cgtcgccaag atgtcaccgg aagtaaaaat cggccagctc atgatgatcg
    11041 gcctggccgg gacgaccatc gacgaccagg gccagcgcca gctggaatac tgccatgccg
    11101 ggaatgttat cttattcgac cgcaatatgg atacacccga acaggtccag catttgacaa
    11161 aaaaaataga cacgacgatc cgcaagaaga gcggcgtcat gcctttcatt gccctcgatc
    11221 aggaaggggg ccaggtcctg cgcatgcgca aggccttccc gcccgtaccg tctgaacagt
    11281 ccatcggcca gagcggccag tcggcccagg cgaaacagtg ggccgtcacg acgggcgatg
    11341 ccctgaaaaa gctgggcatc aacgtcaact ttgcgcctgt cgtcgacctc ggttctcagg
    11401 ctgaacggtc gtacagcctc gaccctggcg tcgtgaccca attcgcccat gaagccattg
    11461 ccggctacga acagacggga atctggtgtt ccctcaagca tttcccgggc atcggcaaag
    11521 tcaagacgga tccccatatc gacggcgaca gcgtcaacag ttccaaggat gacttgatga
    11581 agcaggattt gaagcctttt gaaaacctca tcaagagcga agacaatcag aaaatgttca
    11641 tcatggtctc gaacgtcacc ttcccggcgc tggaccccaa tcttccggcc tgtgtctcca
    11701 agccaatcat gaccgattta ttgcgcaaca ctttcggcta ccagggactc atcgtttccg
    11761 acgatatgga aatgggcgcc atggccaagc actatgcctt tgccgacatg ggcgtcatgg
    11821 ccatcaaggc cggtgccgat atggtcctgg tctgccatga atatgcccac gaacgggaaa
    11881 cgtatgacgg cctcttgaag gaatataaga gcaacgcgga attccgggcc ttagtcgatg
    11941 aaaaagtaac ccgcatcgtc cgcacgaaac tgaatcatca atagctgcct tttcgatgca
    12001 gcaatagata caacgcgccc agcataccgg cgtcgttctg ccgtttggcg aaggccagtc
    12061 tcttaggcgg cagcatcatg gccggcaatc gcgccgccag ggctgtttcc agcttcggcc
    12121 tcaggatatc ctcttgggcc atgatgccgc cgccgaggac gatgccatcg ggattgcaca
    12181 ggcaggcgat attggccagg ccatcggcca gattctggca gaatgtatcg aggacagctg
    12241 ctgccgtggc atctccctga cggacccagg caaagcaagt acggccgtcg accttttccg
    12301 ggtcggcggc ttttttgcat gccatttccc ggcagaggta tgtcgccgaa gcgacgtcgt
    12361 ggaggcggcc ggcagggacg cgcatatagg cgatttcccc ggcagaccgg caggctccgt
    12421 gaaggatatg gcccttttcg atgaaggccc cgccgatact ggtgccgacg gtcacggcga
    12481 agagggagtc catgtcccgg ccggcgccga gccaccattc tcccagggcg gcgcagttcg
    12541 tgtcgttttc gacgatacag gggagctgga attcattttc caagagcgat ttccaggccg
    12601 tccccgtata gtccgggatg gacagggcgt agtcgacggc ccctctttcc gtgtcgatga
    12661 tgccggctgt cgagatgccg atgcccgcta agggatggtc ggccagctgg cgctggatga
    12721 gcgagcggac tttgtggaca atgcccggac cgccgcccgt cgccgcttct gtcggcaggc
    12781 tgcctttctt atggaaccgg cccgtttcgt cgatgaggcc gaatttgatg gctgtaccac
    12841 cgatatctat gcagctgtaa atcatgagta tacactcctt cctgagatta tacataaatt
    12901 atacatagat ttgacctttt ctatgtatag ggactttata cttatcgtgt cgaatatgtt
    12961 acaaaattca ggaggtcacg atatgaaacg acaatggtta aaacgagcaa ttctttccat
    13021 ggtagcctgc ggctcattag caggattcat tcccggagta caggcggcag atgtccgcat
    13081 ccttcccgtc gataatgcga aattcctggc tggtgccaaa ttcgatttcg acatnnnnnn
    13141 nnnnaagaat ctgaagaatg tcgacattac ggtcaatgga cagaaggctg ataaattctt
    13201 tggtcagaaa ttagatgcta aagatttagg caacggcgtc atttcatatc gtgccaatca
    13261 ggtcaatttc cccaaagccg gtgcctatac ggtaaaagcg acggctaccg atgccgatgg
    13321 cgctaattcg gccgatgccc gctataccgt cgttcaggac aaagctcagc gccaggccaa
    13381 gaacgtcatc ctcttcgtcg gcgacggcat gtccctgcag gcccgcgaaa tggcccgtat
    13441 tatttccaaa ggcatgacta acggcaagta caatgactta ctggccatgg aaaaaatgcc
    13501 ccataactgc ctgattacga ccagcggtta cgattccctg acgacagaca gtgccaactc
    13561 ggcgtctacc tatgcaacag gtcataaatc cgtcgtcaac gccttgggcg tctatgaaga
    13621 ctccacgaaa gaccccttcg atgatccgcg cgtcgaaaat atttcggaaa tcgtaaaacg
    13681 cagccgcggc atgtctgtcg gtatcatcac ccaggccgaa tcgacagacg ccacgccggc
    13741 agccatggtc ggtcatacgc gccgccgtgc caaccaggat tacatcgctt cggaatacct
    13801 cgaagattat catcgcccgg acgtcatcat gggcggcggt tcgcgccgtt acatcccgca
    13861 gtccgaacag ggctcgaagc gccatgacaa ccagaacgtc atccaggaat tcaaggataa
    13921 aggctatacc tttgtcagca attctaagga aatgatggcc gctccggata ataaaccgct
    13981 cctgggcctg ttccatccga gcacgatgaa tgtctatgtc gaccgtgaaa tactcaagaa
    14041 cccggaagtc ctcggcaaat atacggacca gccgaacctc attaacatga ccaagaaatc
    14101 cctggatatc ctcagcaaga acccgaaagg cttctttgcc atgattgaag gcgcttccat
    14161 cgacaaacag ctccacgcca tggattggca gcgcgcaacg tatgacacca tcgaattcga
    14221 caaggccatc gaatacgccc agaaatggaa taaagaacac ggggacaaca ccctgatcat
    14281 cgtcgtcgcc gaccatgctc acggcgcttc cattaccggg acctaccatg aacgggatgg
    14341 caagaagggc ctggcagctg tccgcactta tgccgactcc atcttcccga ccttcaaaga
    14401 tgccaaccac gatggcttcc cggataatcc tgatcccgat gtcaccctgg ccatccagta
    14461 tgccaaccat ccggaatatt acgaaaactt ccacttccag cagaaaccga cgctgccgac
    14521 tgtttctcag accgttacca aacaggaagc tcagcaggct ggcgataaga ccgaatacca
    14581 tcaggtatcg acagtcgtcg aaaaagccaa tccggcccgc catcacgacg gcgatccctg
    14641 ggaactcgta ccggccaaca ttcccgatac catcggtgac gaatgccatg ctgccgacga
    14701 cgtaccgctc aacgctgaag gtcctggctc ggaatacttc cagggcgtca tggacaacac
    14761 cgaagtattc tttggcatca tgagagccct cggcatcgat gctacgaaaa atgtgaaata
    14821 attagtggct cgtggcccgc agtggctctc ttcataggag agctgtcagc gaagctgact
    14881 gaaaggttgc tcttttatag tttgtaattc ggagtatgta gggggcgcca cgtgggcgac
    14941 ccgttaggga tttggatgat tcttctagca aacagggcag gaaatcgtac atatcccaga
    15001 gcgggcgccc cacgtggcgc ccctacaaga aaaggcgctg tcttcgtgcg acagcgcctt
    15061 tttattattt ccctaacacg ccttccagga aggaagcggt atctgccata tagctggctt
    15121 catagccttc gatatcgcct ttatcgcagt gggctgcgaa ttccgggtcg atcggcagtt
    15181 ttgccgtatg ggggatatca tatttctgtg ccgattcttc gacgtggctc ttgccgaaga
    15241 tggacagttt ttcatgacaa tgaggacatt caaaatagct catgttctcg atgaggccca
    15301 ggactgggac gtgcatcatt tccgacatgt tcagtgcttt ttcgacgatc atggaaacga
    15361 gttcctgcgg cgatgtgacg accaggatgc cgtccattgg cagggactgg aagaccgtca
    15421 gcgggacgtc gcccgttccc ggcggcatgt cgacgaacat gtagtcgata tcgccccaga
    15481 tgacatcggt ccagaactgc tttaccgcac cggaaatgac cgggccgcgc catacgacag
    15541 gagccgctgc gtcttcgagc aggaggttca tggacatgac cttgatgccc gtcttggtcg
    15601 ttaccggata gataccgttt tcatcgcccg tagcgtggtc ggtcaggccg aagactttcg
    15661 gaatcgacgg gcccgtgata tcggcgtcga ggatagccgt cttgaagccg cggcgctgca
    15721 tttcaacggc cataagggac gttaccaggg atttaccgac gccgcctttg ccgctgacga
    15781 cagcgatgac atgcttgatg tgcgagccgg catgaggggc ggcctgcagg ctctggggct
    15841 gtgtgcgttc agcgcaggag ctgccgcagc tggaacagtc gtgtgtacaa ttttcactca
    15901 ttgggatcat tctcctttgt attctttttt ccggcgcggc agcaggtatg acaacacgtg
    15961 ccggaacgtt cacaaacttg gatatggccg ccttcgatga cgaggcgttt gccatggatg
    16021 agggcgtcgg ccaacttgtg tcgggccgaa tcataaatgc cggttaccgt cgtgcgcgcc
    16081 acattgatct gagcggcaca ctgttcctgc gtcaggccga cccagtcgat gaggcggatg
    16141 acttcgtatt cgtcaatgcc catgacaatc acatcgtctg actgtgcgcc atctataggc
    16201 tcgaaagccg ccgaaacagg caacgaacag acccgtcggc accgcggtgg tctcggcata
    16261 gtaccccctc ctttacgcct tttattatag atttatagat gacatatgtc aacattgtaa
    16321 gggggctatg acgcattttc ggattttggg agctgccctg gggaaggatt gtgaagtcgt
    16381 ccttcatgat ctgcgttatc ccgatgaatc ggtcatcgcc attgccaatg gcgacatcag
    16441 cagccggaaa gtgggggcgc cggcgacgga ttttattttg aaactcatgc aggtcggtaa
    16501 gaaaagggac caggaatata tgaccaatta ctacgggaag agcgtcaacg gccataccct
    16561 gcggtcttcg acttttttta tccatgatga ggaggataat attatcgggg tcctctgtct
    16621 gaattacgat gtccagcgct atctggacgt gcggaaacaa ctggatgcct tcatcttgat
    16681 ggacccgggg aagcatatcg acggcatgcc tcaggataac gctaagaagg aagatgacca
    16741 tttcctgagc gtcgaaatct cagaaagcat gtatccgacc gtagacgatg ccatccagca
    16801 tctcatccag cggaccctgg ccccttacgg gaccgaggcc aaacgcctat cccaggcaga
    16861 acgcctggct gtcgtcgaag acctctacaa gaacggcctc ttcgtcctca aaggcagcgt
    16921 ctatgccctg gcccagatcc tgggcgtttc ggaaccgacg atttaccgct acttgaaccg
    16981 tatcaagaaa gaaaatcaca atgcacaggg gagagaaaaa tggatttagg gaattggaga
    17041 gacttctttt catctaaaat ttacaagcgt ggcgaacgct attacgaaga aggcgacgtc
    17101 tccgatctga agtgcacgga gaagcagata acggccgacg tctatggcag tgatatatat
    17161 gaagtcacta tcgatttgga cgacggccag gtcgaaggga tggactgcac ctgtccctat
    17221 gccgaacagg gagaatattg caagcacatg gtcgccgtac tgctggccgc tgaagacgaa
    17281 gaactggatg aagcggctga tgctggacag aaaaaagcat cgtggcagga tatcttgcag
    17341 cgcctgtctg aatcagattt gcgtaacttt gtctttcaat tagccgaaga taatcgggat
    17401 ttgcagaaga aaatcgtact agatttttcc aggggcaaac cgtctgctca ggaaatgcat
    17461 cgatatgaac gcgaaataga ccgggtattg gctccttacg acctcttggg atatattgaa
    17521 tataatgcca gtaaggcttt cgctgacaag gtccttgatt atttgagcag caccataccg
    17581 ccgctgatta agcgctcttg tacgatggaa gcattggaac tggtcaatat catggcgtcc
    17641 aatgtggcgg atatatcttt tgaagatgaa gatgaccagc agctctgtct ggatgaatgt
    17701 cgaacttact gggccgatat cattggtcat gccaaaccgg aagacgtgcc gaagctgcat
    17761 caatggttta tacagcagat ggatgcaatc aaaaacaagg gcaatgagct atacggctat
    17821 tacgaagagg tgctgtataa tgacttcgat gacccggcgt ccttgaaatg gaaacttgaa
    17881 cggattgatg cggaaatcga gcactatgaa tcgacaaaat ccgaccaaaa cgattctgta
    17941 ttgtttattt gaaaaaactc agcacctatc ctgaaggcct ggacttggcc cgcaaaatcg
    18001 ctcaagaatg gtgtgaaacc taccggaacc gccgggccat gaaagacgaa ctgtccaaag
    18061 caggcttcta aaaaggctcg cctcatagga gagctggccg cctgaggcgg tctgagaggt
    18121 tgaagttttg tcggtgtccc ccttgataat agcttttgac aaaaagtcgt gcggaaaaat
    18181 gtagtaggaa tacaaaaagt cgcgcagaaa aatgtagaaa tagacgcaaa agtcgcgcga
    18241 aaaaatgttg ttgggcttta tatagaaaaa tcccctgatg ctgtccacta tttcgatagc
    18301 ctcgggggat ttttttagtc gatgttgata
//
LOCUS       DAWABZ010000022        18163 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_843_length_18163_cov_3.226585, whole genome shotgun sequence.
ACCESSION   DAWABZ010000022 DAWABZ010000000
VERSION     DAWABZ010000022.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 18163)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18163)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18163
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_843_length_18163_cov_3.226585"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6209..6233
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8376..8385
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3779 a         5062 c         5294 g         3993 t
ORIGIN      
        1 gtcttcggct gcatagggat agggaatgag gatggacggc acgccgcgga cggtcagttc
       61 agccagaccg acggcaccgg cccggtagac ggccaggtcg caggcggcca gggcgtcggg
      121 catatgatgc aggtacggga tgatacggct tcccttgcca tagcggccgc ggcattcgat
      181 gcccggcagc tgcttgacga cgcggtcgta ttcatggtcc cccgtgacgt ggagaatctg
      241 gatatggtcg tcgttgcgga aatacttgtg gacttcgatc atcgccgtgt tgatgctgcg
      301 ggcgccgcgg ctgccgccgg cgacgaggag cgtaaactta tccgggtcca ggcccagggc
      361 cttgcgccct tcttcccgcg tcaccgtgag gatatccttg cggacgggat tgcccgtata
      421 gaccaggata tccttgcgct tgaaacgggc cgctgcctct tcatagccca gggctacgcg
      481 gttgacgaag cggctcaaaa tcgtattggt aacgccgggg atgacgttct gttcctgtat
      541 cagtgtgggc acaccactca ggctggctgc caaaaggacc ggaccgcaga cgaaaccgcc
      601 cgtcccgatg acgacgtccg gtttgaattt gtggataatg ccttctgcct tgatcagact
      661 gcctgccgtc ttgcccaggg tgacgaggtt gcggacggaa atcttccgtt ccaggccccg
      721 cacgtcgagg gtgacgaaag gcaagccttc tttagggatc aatgtatttt ccaggccgat
      781 ttgggagccg acgtagagaa attcggtggg ctcaatgtcg gcaatcgccc gggcaatggt
      841 aatcgccggg tagatatggc cgcctgtgcc acctccggaa ataatgactc gacgcatgtt
      901 cctgcctcct gttgtttctt ctaaatatcg ttaaatggcg ttgacgatgc ccttgaagac
      961 acgtccgcgc tgttcaaaat tatcatacat gtcaaagctc gagcaagccg gcgacaagag
     1021 gacggcctgc ggtgccttgg caattttgcg ggccagcatg atggcccctt ccatgctgta
     1081 accagtgcgg cggatatcct ggatacccgc cttcttggca gcggcttcga agcgttcggc
     1141 cgcatcgccg atgaggatca gcgtatcgac gcgtttcgcc gccaaggcca tgaagtcatc
     1201 gaggggcgtc ttcttgtcat agccgccggc aatgaggatg aggtgcccct gcgggaaggc
     1261 ttccagggcc ttcatggacg catcgacgtt ggtccccttc gagtcgttgt aataggtgac
     1321 gccgtggatg gtccgcactt tttccaggcg gtgttcgacg cctttgaaag ccttgaggac
     1381 ctggcggatg acgtcgaggg gcgctccggc agcataggtc aagagaatcg cagccaggca
     1441 attctggata ttatgcttgc cgaagaggtg caggtccgtt tcggcacaga cgacggtatc
     1501 ctggccatgg aggcgcagga taagctgtcc gtcccgataa aaggcccctt ccgggacttc
     1561 gccgcacgtc gagaaataga cgaccgtacc ggtcatgtct ttcgccatgg cccgtacccg
     1621 ttcgtcatcg taattgagga cagcgtaatc gcccttgtcc tggttgagga aaatccgttc
     1681 cttggcagcg gcatagcctt ccatcgtatg atggcggtcc aggtggtccg gcgtgatgtt
     1741 gaggattgtc gcggccttgg ccttgatgct gtgcgtgaat tcgagctgga aactggacag
     1801 ttcagcgacg acgacggctt cttttggcag gttgtcggcc gtagccgata ttgccgccga
     1861 cgacggtctg acggcctgtg gccttcatga tttcgccgac cagggtcgtc gtcgtcgtct
     1921 tgccattggt cccagtaatg ccgatcatgt cagcatcggt gacccggcag gcgacttcga
     1981 cttcactcca gattggcagg ccgcggcggg ccgcttcttt catgatcggg atttccggag
     2041 aaatgaccgg cgacgggacg acgacatcca tgccgtccaa aagagccgtt tcctggctgc
     2101 cgaagaccag ctggacgccg gcggccttta tatccggcca cggctgttcc gccgtatcga
     2161 tggccttcat atcattcagc gtaacggacc cgccatgacc tgccagggcc agagaggcag
     2221 cgcggccgct gatgcctgcg ccgatgacta agaaatgttt tccagaaaaa tccatgatgc
     2281 tcattgccaa cacctgcttt atataaaata tcgtagttca atcagaaaag ccgcaggccc
     2341 tatttgcagg ccagtacgac gataatggac aggatcgcca tgagagccga gaagctccag
     2401 aacacagtga cgaccttgac ttcactccag ccagacagct caaagtggtg gtggatcggg
     2461 ctcatcttga agacgcggac accgcgggtc ttgaaggaaa tgacctggat gatgaccgac
     2521 agggcttcga tgacgtacaa gccgccagcc aggatgagga gcagttccgt cttggtcaaa
     2581 atggccatag ctgccagggc accgcccaga gccaaagagc ccgtatcgcc catgaacatc
     2641 ttggccggat gctggttata gaagaggaag cccaggcagg ccccggtcag ggccgtgcca
     2701 aagatagcga aatgggtata gcccagggcg acggcgatga cgacgtaggc aacggacgta
     2761 atggcgcagg acccggcagc caggccgtcg aggccgtcgg tcaggttgac ggcattgctc
     2821 gtgccgacga tcatgacgaa gacgaagaga tagtagaaca tgcccaaatc gacagtgatg
     2881 gaagtaaagg gaatccacaa ggtcgtcggg aaatggagaa aggcatcgag gatgtagcag
     2941 aaaatcgcgg ccatgacgaa ctggccggcc agtttctgtt tcgccgtaag acccaggttg
     3001 cggtgcttga tggccttgat gctgtcatcg aggaagccga tgacgccatg ccccaaagcc
     3061 aggaataagg ccaggccttt gcgcaggtcc atcggatcga agatgaggca cgatacggtc
     3121 agcgccgtca ggatgaggat gccgcccata gtcggcgtcc cggctttata tttgtggctc
     3181 tgcggccctt cttccctttc ggactggcgg gcatggaatt ttttcaattc cttgatgacc
     3241 agcggtccga agaccaggcc gatgaccagg ctgacgatga agctgagaat cagcatttta
     3301 aacattcaca ttacttcctt tcaaaatagg gcagtatttt ttccatggca aagctccgcg
     3361 agcccttgag gaggactgta tcgccatcgt ggaggagttc tttcagctgg cgcgccgcct
     3421 gttcatgcgt atccgtcgta tagacggccg tcatgcctgc agctttcgcc gattgagcga
     3481 tatctttggc taaatcgccc atcgtgatga gggcatcgag accggtctgg gcgacgaggc
     3541 ggccaatggt ttcgtgttcc cgtttcgccc agtctcccag ttcgcccatg ccgccgagga
     3601 cggcgatctt gcggcccttt gtcagcaggt ccagcgtgcg gatggcttcg gccatggacg
     3661 cgggattggc attatacgta tcgttgatga aggtatacgg cccgatatgc agcagttcct
     3721 ggcgcatggc catgccgtgg tacgatgcca ggccgcgctg tatctcagcc gcgctcaggc
     3781 ccgtcagctg gccgacggca atggctgcca gggcattgta gacgttgtgg ccgccgatga
     3841 ccggcacgga aacgtcgaaa gactggccgg cgccgctgca atggaagcgg acgccctgct
     3901 ggtcgcagac gatatggtct gcccgtacag ccgcctggtc atcggtccca tagaggacag
     3961 tcttcccttt gcacaaagcc gtcatcgggc ggacgaaggg atcgtcgccg ttgaggacag
     4021 ccgtcccctt ttcatccagg gcctggacga gttcggcttt ggccttggcg atattttcct
     4081 gcgagcccag cagttcgata tggctcttgc cgacgttggt tacgacgccg atagtcggtt
     4141 cgccgatggc agccagttcg gcaatctgcc ctaagccgcg catacccatt tctacgacgc
     4201 aggcttcgtg gtccggcgtc agctgcaaga gcgtcttggg caggccgatt tcgttgttga
     4261 aattcttttc cgtctgcaag acacggtact tttcgctgag gacggtagcg atcatattgc
     4321 gcgtcgacgt cttgccgacc gaaccggtga cggctacgac ggggatggca aagcggcggc
     4381 ggtggaagcg ggccaggtcc tgataggcct tgagcgtgtc ggcggcgacg aaaatcggcg
     4441 ccgtcagccc ttcatattcc ggacgcactt ccgatacgac tgccccggca gcgccggcag
     4501 ccagggcctt atctacgaaa tcatggccgt tgaaagacgt ccctttgaag gcgacgaaca
     4561 acgaaccctt ggcgatggcc cgcgtatccg tgcagacatc gctgaaagac acagaggcgg
     4621 ccggcttcag gagccgggcg ccagtggcct ggattatttc ttcgctagta aaagcagcca
     4681 ttatttatct cctctgtgca gtgcatggac ggcgttgcgc gcttcttcgc ggtcatcgaa
     4741 atgaatggtc ccgtcgttga ggatctgata cgtttcatgg cccttgccgg cgatgacgat
     4801 gatgtcgttg gtctgggcca gttcgacggc gcggtaaatg gcgtcgcggc ggttgacgat
     4861 gacttcatgg tgctgacccg gtttcaaacc ttccttgacg cccgcttcga cttcggaaac
     4921 gatggtttcc gggttttccg agcgggggtt atcagacgtg acgataggga tatcggccag
     4981 ttcggcgccg atgcggccca tgatggggcg cttggtctta tcgcggtcgc cgccgcagcc
     5041 aaagacggcg atgatgcggt tcggcttcat ggcccgcgcc gtttccagag ccttctgcag
     5101 gctgtccggc gtatgggaat agtcgacgac gatggcgaaa tcctgaccgg cttcgacgag
     5161 ttcaaaacgg cccggtacgg agtgaaaggt ctggatagcc ttgtcgatag tggcttcatc
     5221 gacgccttcg gcatgggctg cgccgatggc agccagcgta ttgtagacat tgaacaagcc
     5281 cgtgatattc atgttgaggt ggacgttgac gaaggggccg acgacatcaa aggaggattt
     5341 cttcaattcg actttcaagt tggacccttt gaggtcggca ttcttttcga caccgtaggt
     5401 caggaccttg cagtggcagg cctggaggat atccttggac cagggatcgt cgatattgac
     5461 gacagccgtc ttgttttcct tgacgtggtc ggcagcggtc aggctctgga agagcttggc
     5521 cttggcggcg acgtagtttt ccatggtctt gtggaaatcg aggtggtcct gggtcaggtt
     5581 ggtgaagaca gccgtatcgt attcgatgcc ggcgacgcgg ttcaaggcca gggcatggct
     5641 ggaaacttcc atgacgacat gggtcatgcc cttttcctgc atgagggcca ggaagtgctg
     5701 gagttcgacg acatccggcg tcgtgttgtg gatcggcagt tcttcatcgt cgatgagggc
     5761 gtggatggtg ccgatgacgc cgacatgata gcctgcagcc cgcaggatat gggcgatgat
     5821 gtgagtcgtc gtcgtcttgc cgttagtgcc ggtgacgccg atcatgcgca tcttcttgcc
     5881 cggataatca taaaagaacg ggactatgtc ttccagggcg tgatggatgt ccggtacctg
     5941 gatctggata gtgctcttgg gcaggtcgag gtgtttcgtc gtcaagacgg cgacggcccc
     6001 tttttcgaca gcctgggctg caaaggcggc gccatcgacg tggacaccgg aaatgcagac
     6061 gaagagatag cccgcttcaa tctggcgcga atccgatgaa ataccggtga tcaccgtatc
     6121 ggcactgcca ttgagattat aaatgccctt gatctgttta caaagttctc ctaccgtttt
     6181 ggtcataatc ataggtcctc cttgtgacnn nnnnnnnnnn nnnnnnnnnn nnnatacaag
     6241 ctaaacaaca atgaggtcgt cgcacgaagt gcgtcgacct ctcggtttta agcaattatc
     6301 gacgttttcc ccttgcggcc tggtccgtct ggctcccgta aaagatccgt gacggtgcta
     6361 ccatgcccag tcgcttcgtc gcggtcgtgt aaattcgttc cggtgtttcc aatttggaaa
     6421 tctcgacgcg cagttcatcg ttttcacgct gcagctgctg cgtctcgcgc ttcacctcca
     6481 tgagttctcc actctgctga tacgtccagg atgtcagtag attggacacg atcacgatag
     6541 ccgcaatcat tccaatcggc agcaggacgt aagccgcccc ccgccggatt gcgtctacgg
     6601 cagccgtccg ccataagggt atgctcttat caccatgtat gttcccggct ttcctcacca
     6661 gcatacgcaa cacatccatt tccatgatca tagcccggcc ccgttacttg cggatgccga
     6721 cgcggagctt cgcactgcgg gcccgcggat tcgtatccag ttcttcctgc gacggcagga
     6781 tgggctggcg cttcgctttt aggatggcat gatgatggca cgtacaaacg ggcatgtccg
     6841 gcgggcagat acaatctgtc gccaagagcc tcaacgtctg tttggcaatg cggtcttcca
     6901 gggaatggaa ggtaatgacg cacagccgtc cgcccggctt caggcgctgg gcagcctgtt
     6961 ccagggaatc gtgaaggatt cccagttcat cattgacttc gatacggatg gcctggaacg
     7021 tccgtttggc cggatggggt ccgtcctttc gggcaccggc cgggatggcc ttcttgatga
     7081 cctggaccag ttcttctgtc cgttccaagg gcttttcccg ccgcgcctcg acgatgaagg
     7141 ccgcaatgcg cttggcccac cgttcttcgc cgtaggtctt gatgatgcgg tataagtcgg
     7201 cctggctgta gccattgacg atatcgtaag cgctgcgctt ctggtcccgg tccatgcgca
     7261 tgtccagggg accgtcctgc atgtaggaaa agccccgttc cggcgtatcg agctgatagc
     7321 tggacacgcc gaggtcgaac atgacgccgt ccaccttttc tatccccaat tcatcgagca
     7381 cgtctggcag atagcggaaa ttccgctgta ccaggatacg ccggcaggcc gtatccttca
     7441 gccgttccgt cgccgctgca atggcgtccc ggtcctggtc gaggccgatg agcgtcgctt
     7501 taggcgacaa gcgttcggcc agcgctcggg aatgaccacc gccgccgagt gtacaatcga
     7561 catagatccc gtcagggtcg gtaattaagt tatctatggt ttctttacga agcacactga
     7621 cgtgatgaaa attcataatt cctcctaaaa gtccaagtcc agggcatcgt cgagactttc
     7681 cgcaatttct tccatgaccg gtacggtctt ggcagcatac gcttcataag cgtctttgct
     7741 ccagatttcg accttatccc ctgtccccag gacagtcaca tccttgacca gcttggcata
     7801 ggccctcaat gtcgccggaa tcaggatgcg gccttgttta tcgaattcta cttcggcagc
     7861 actgccgaag acaaaccgct tgaaggcgcg gacattttct ttagaagccc ggagtttttt
     7921 caaactgccg gccaaccgca tccacgcctc catcgtatag acagaaaggc agccttccaa
     7981 accgcgggta acgacgaact ttggccctag ttcgtcccga aatttgacag gcaaaataac
     8041 gcgacctttt gcatcgatgg tatgttcata ttcacccata aacataaggc cgcaactcct
     8101 ttcacttttc accacatttt accacatttc cacacgccgt gacaacataa tggcgaaaaa
     8161 aagaagtttt gcctgaaagt ttcttctttc agacaaaact tcttggggag attcgtggtt
     8221 cgtagttcgt agtccaagtt aaaagtttgc agtttgaagt ttgtagtgaa tagttttaaa
     8281 tttaaagcca tcttctacaa actccgtact acaaactata gagaaaagta acttcgcata
     8341 atgacaggaa cctgagtgcg tctcctctgg cgggcnnnnn nnnnncctaa agcatgttta
     8401 cgacgatggg ctcggtgcgc aatttgtgga aatcgcagcg ccggacaagt tcatcccggc
     8461 tatagcggcg ccgttccggc accaggccgc cggcgtaggc caggtaggat aagatgtctt
     8521 ctgccgtaag gccccggtcc cgcaaggagc ggatagtgat gccgtgctgg cgcttggata
     8581 ggcggttccc gacttcatcg acgagcatgg gcacgtgggt gtatacgggc ggcgtataac
     8641 ccaacgtttc catgagccat atctgctggg ccgtcgacga taacaggtct tcgccgcgca
     8701 ggacatgggt gatggccatg gccccgtcgt cgacggagac ggccagctga taggcataca
     8761 tgtcgtcggc ccggcggacg acgaagtcgc cgcagcagcg cgtcaagtct tcgtggaacg
     8821 gcccgtagac gccgtctgta aaggaaatgg tccgttccgg cacatggacc cgccacgacg
     8881 gcttcttggt catctgcttc cgctgggctt cgctcaggcc gtagcaatgg ccgtcgtaga
     8941 cggtatgctc gccggcatga ggggcgccga cggcctgcag gcgggcgcgg ctgcaataac
     9001 agggatacaa gaggccggcg gttttgagtg tatccagggc cgccgcatag cggtcgtatc
     9061 gttcctgctg gatgtacggc ccgtaaggtc cgcctatgtc aggcccttca tcccaatcga
     9121 ggcccagcca gtgcaggtct tccatgaggg cctgggtaaa gatgggtttc gatcgctgtt
     9181 catcaatatc ttcgatccgc aggaccaagg tcccgccggc ctggcgcact tgcagccagg
     9241 ccaggaaagc cgaccagaca tttcccaaat ggatatagcc cgtcgggctc ggcgcaaaac
     9301 gtccgcgcat gacactcaca tcctaatcgg gcagcgaaat gaggctggtg aacaagtcct
     9361 tcgcttcgcg tatcttggct tcactgccca tgacgcagac attgtcatcg gccatgacgg
     9421 cgcggatgcg cggtgccaga gcccggatgt cggcggccgt cgtcgccagc agctggtccc
     9481 ggcgctgctg agcaatggca tcagtggtcc ccgtgaaata gcgggtcatg gccttggccc
     9541 cccgcaggga cggcgtcaag gggatgtcga tgcgactcaa ggtcccgatg acgtatttcg
     9601 tcatttcccg gtcagacacg tcgaaggatt ccagataatc tgccagggca tcgtaggctt
     9661 cgacgctgga gcggagattg gggtcgcggt acgagcagaa gaccatgtcg ccgttgtcat
     9721 agaaacgggt gaaggcgccg tacgccccgc cctggacgcg gatcttcgtc cacaagtagc
     9781 cgtactggag gatcgtatcg aggaccatga gcgagccgtc gtagtcgtag ccgtggctgc
     9841 ggaagttgcc gcctttggcg acgtactgga ccgtgccgct ggtcatgatg ccttcattct
     9901 tccgggacag gccgaaatcg cacaagccct ggagcttttc gccctgcggc agggccgtga
     9961 tccacgacga cacagtgttt ttcgccgtct gccagtcgtc ccggctggcc gtcacttcca
    10021 gggtcagggc cgaacgggtg aagatcttct tcatgaccgc agcgagtttg gcgccgatgg
    10081 cttcggcgtc cgtgtggatg cggctggcga catcggtaat gaattcgtag tacgacaatt
    10141 ccccggcgtc gcagaaggct tcgactggcg aaacgtagga caagacgcgg tgcatgacga
    10201 tcgtatggcc ccggcggaag gcatccatat cccagccggc cttcgtttct tccacgagtt
    10261 cgaccaggcg gtccttattc gtgaagaccg tatgcaggga gatttccttc aacaaatcga
    10321 cgagtttgtc caggttctgc gtcaggcctt tggccttgaa gcggaagacc ggcatgtagt
    10381 ccttgttgtc cgtccggttc gaaaaggccg acacgctgta gtcgatgccg cccgtgtaca
    10441 agtcgatgag cgacgcgatg tccccatacg tatggtctgt cgtatccata tcgccgatga
    10501 ggtcgctcag caggtagaca taggaaattt cttcccgtgt caggccgtgg aggtcgaagt
    10561 aggcattgac ataagtaatg ccgttcgttt catccgcgac atagcagacc gggacgccgc
    10621 ctacatcctg ctgttccatg gcaatcgtgt cggccttctt ttccaggtcg tcccgggtca
    10681 gggtcggaat cgtttccagg gcttccggcg agtccggcgt ggcctgccgc ttctgcaggg
    10741 cctgggagtc ggctacgatg gcgtcgattt cttctttcga gagcgaagcc ttgtaaacag
    10801 ccagcttgtc ggccagggcc ttgtcgtgcc gttcggtcag gcccggttcc ggtaccagtg
    10861 agatcagggc gtaataggga ttgtcgagga tgtatttctg gatgaggcct tcaaagtagc
    10921 cgttgtcgat gccgcggcgc aacacatcca gggagccttc ataagacagg gcagccaggg
    10981 gatccatatc gtagagccac gtatccatgc agcggatgcc gtagatgagg cccttcgggc
    11041 ggccgacgaa atcggcttcg cgcaggatga attccgtccg gttcaaagcg cctgtgagca
    11101 gggtcttgtc caggccgtcg cggaccatgt tgctgaggac gtcgcggacg atgggcagga
    11161 tcttgtcctg tttatccggg tccgagccat tgacggaaat gccccacatg ggctgcagga
    11221 gaccgtcctg gaagtcaccg gaaatatcct tgcccagacc ggcatcgacc agggctttct
    11281 tcagcggcgc tgccggcgac tgcaggagga cgtaggtcag gactttgaag gccatggcta
    11341 ccgtcgggtc cagggcgtcg tcgatgacat aggtcaggct gtgcagcgtc ttgtggtccg
    11401 tcttttctcc cgtggcaatg ccgtagggga aggtctggac ctggctgccc gggcaggcct
    11461 ggcggccgat ggccgaatcg atggtctggg cctggaactg gctcaggtat tcgccgtcga
    11521 tgaaggccag ggtctggtcg atatccatat cgccgtagag gaagatatag ctgttcgacg
    11581 ggtgataata tttggcgtgg aaagcggcaa agccttcctg cgtcaagttc gggatatcat
    11641 cggggttgcc gcccgattcg acgccatacg tcgtatccgg gaagagggca tccatgacgt
    11701 gccgttccat ctgcgagtcc ggtgaagaga agacgccttt catttcgtta tagacgacgc
    11761 cgcgataagt cagctcgtcg tcagccgagt cgagatgata atgccagcct tcctgcatga
    11821 ggacctgttt gtccttaatc atgttcggga agaagacggc gtcgagatag acgtccatga
    11881 ggttcttgaa atcagcagcg ttgcggctgg ctacgggata catggtcttg tccggaaagg
    11941 tcatggcgtt gaggaaggtg ttgagcgagc ccttcaccag ttcgacaaag ggttccttga
    12001 gggggaactt ccgcgagccg cagagcgtcg aatgttcgca gatatggggt acgccagtgc
    12061 tgtcggacgg cgtcgtccgg aaggaaatgg aaaagacctt attgtcgtcg tcattctgga
    12121 tatataacag gcgggcaccg gactggacgt ggtgcagctc ataggcctgg ctgtgcagtt
    12181 ccggtacgta acgggaccgg tcgacggcaa agccatggat catctgtttt tcttgtaact
    12241 tcataaaaat gctccttcat ctcttattat ataatgtatt atagctagca tcatgcttgc
    12301 aaaagtaact gtttcaagtc ccctacggtc gctgccgtgt aatcgggctg gaaggccttc
    12361 cgttccgctt catcgccata gccataggtg acataaatga cgggcaggcc caattcgtgg
    12421 gcgccgatga tgtcgaaacg gcggtcgccg accatgacgg cgtcgccctt ttccaccggg
    12481 cccaggccag ccaggatgcg ccgcatgacg tcagccttgg tatcagtccc gccgacggcc
    12541 ggtccggcaa tggcttccac atagggatcg atgccgaatt tctggcagat tgcctcggca
    12601 tggacctggg gcttcgatgt agcgacgaac gagcggatgc ccgcttcctt catggccttg
    12661 agcatgggaa cgatgccgtc gaaagcccgg ttctcgaact tgccgatcgt gctgtagcgt
    12721 tcctggaaat aggcataagt ctgacgggcc ttttccggcg aaaagccata gacgtcttca
    12781 aaggtcgtca acagcggcgg cccgacgata gtatgctgat ccagacggcc ttctgtcgat
    12841 tcgcccatct gttccagggc ataggcaacg gacttgatga tgccttcctt ggaatcggtc
    12901 agggtcccgt cgagatcgaa taaaacgtat ttatacatgt aacttctcct gtgaaactat
    12961 acttattgtg taccctatct attttagcct gctttgtcaa tatttgcaat catttataca
    13021 gggcaaagac cgtgaaaaaa ttcccattat tgaccgtttt agtatactta tggtataatg
    13081 actttacatt aacaaagaat cattttctct tatgtccatt acgtagaaag gaagtgtcct
    13141 gatgaaaaat gtcatccgcc cctcttacga tttatatatc aatggccgct gggtcccgtc
    13201 cagcgacggt tctaccttta aagccatcaa ccctgccaac ggtgaagtcc tgtcggtctg
    13261 tgccgaagcg acgcaggacg acgtcaacgc cgctgtccgc gccgcccagg ctgccttccc
    13321 ggcctggaag aacgtatcgc cccgaaaacg acaggccatc ctcttgaaaa tcgccgacat
    13381 catcgataag aatgccgaca cgctggccct catcgaaacc ttggacaacg gcaagcccat
    13441 ccgggaaacg aaggccgtcg acatcccggc agcagccgac catttccgct acttcgccgg
    13501 cgtcgtccgc agcgaagacg gccgggcgtc gaccctggac gaccacacgc tgagcctggt
    13561 catccacgaa ccgattggcg tcgtcggcca gatcatcccg tggaacttcc ccttcctcat
    13621 ggcctcctgg aaactggcac cggccctggc cgccggctgt acgatcatcc tcaagccatc
    13681 gagcacgacg tccctcagcg tcctggaact gatgcacctc ctggaaggcg tcctgcctga
    13741 cggcgtcgtc aacgtcatca ccggcaaggg ctcccggtcg ggtcagtaca tcctcgacca
    13801 tccggatatc cagaaactgg ccttcacggg ctctacggaa atcggccgcg gcgtctacaa
    13861 agcagcccag gaaaaactca tcccggcgac actggaactg ggcggcaaat cggcgaatat
    13921 cttcttcgac gactgcgatt gggacatggc catggacggc gcccagctgg gcatcctctt
    13981 caaccagggt caggtctgct gcgccggctc ccgcatcttc gtccagaaag ggatctacga
    14041 caagttcgtc gccgaactgg ccgaacgatt caaccgcgtc aaagtcggcc tgccctggct
    14101 ggaagacacg cagatgggcg cccagatcga cggccgtcag gtcgaaaaga tcctcagcta
    14161 catcgaaatc ggcaagcagg aaggcgcccg cctggtctgc ggcggcgaaa aggcaaccgc
    14221 caacggcctc gacaagggca atttcatcaa accgacgagc ttcgccgacg tcaccaacga
    14281 tatgcgcatt gcccaggaag aaatcttcgg ccctgtcgtc tgcatcctgc cctttgagac
    14341 ggaagacgaa gtcatcgccc tggccaacgc cagcgaatac ggcttaggcg gcgccgtctg
    14401 gacgcgcgac atcaaccggg ccctgcgcgt agccaaaggc gtggaaacgg gccgcatgtg
    14461 ggtcaacacc tacaatgccc tgccggccgg cgcacctttc ggcggctaca agctctccgg
    14521 catcggccgc gaaacggaca agacgaccat gcgccattac atgcagacca agaatatctt
    14581 catcaacctc tcggaaaagc ccagcggctt atacgaataa gtaatcacga aaggacactt
    14641 ctcctatgac agcaaaaaaa tcatctccct cctgctcatg ctgctcctgg cctgtacggc
    14701 cgtctacggc tttgccaccg tcgaccggcc ggctccgccg caggctgcga ccggtgccag
    14761 caccaagaaa taacgattga caaggacctc ctttcttcct ataataaagc cattgcagag
    14821 aagaaaggag gtttttccat gaaaaagtta ctgtcgaaga aagaaaaagc ccggcgccag
    14881 agcatccgcc ggtcaccgca gatcaaacag gccatccgcc gcctcgaccc ggcgcgcatc
    14941 ctgacgctgg acctggagac gacgggcctc acggccgacg ccgaaatcat ccagctgtcc
    15001 atcatgaacg gctgcggcga cgtcctcatg aaccgctatt tcaagcccct ctacacggaa
    15061 cgctgggatg aagcgatgga agtcaatcac atcacgccgg aacaggtcgc ctgtgaagac
    15121 ccgttcatcc tctgggccga cacggtcagc agccttttca tggatgcaga cctcatcgtc
    15181 ggctacagta ccttcaacga cattgccaag ctccacgcgt cgggcgtcgt cctgccggac
    15241 aaggacatct acctcgacct ggccgaagcc ttttccctca tccattacca ggaaacgaag
    15301 accatcactt atgaaaagct catgaactgc gccgcccact acggctatca cggccgcaac
    15361 tggcacgaca gcctgaccga tacggacgcg acgctcttct gcttccagca catgctcgac
    15421 gacgaccagg ccatcttcaa aaaacgccgt tacccgcaaa tcagcggata acggcgtttc
    15481 gtcgtcccag ctagacgccc cggtcgtcgg ggttccagat gatcttatgg agttgaagct
    15541 gcatgtgcca gtccgtcaga cggtgttcct tcatgaactc gacgatgcgc cgcggctcga
    15601 ttttgccgaa gacggcgccg acgtagacgg cacatttagg ccggtacagc tgatagacac
    15661 ccagagcctg gcgcaggtcg gcttcgtcgc cgacgacgaa cttcaggacg tcctgaggcc
    15721 ggagcttctc gaagttctcc ggcagcatgc gattggccat gtccgaagac aggctcttat
    15781 aatcgacagt gaaccagacg tggtcgtatt tgaaatacgg ctcgatgtcc atgctgccat
    15841 tggtctcgat attgacgctg tgccctttga gcccttccag gagggcgtgg atgtcctggc
    15901 acagcggttc gccgccggtc agggtgacgt tgggatagga cacttgggcg atgatatcgg
    15961 caatgtccat gtgccggcct tcgttaaagg cataggccgt atcgcagtaa gaacagcgca
    16021 ggttgcagcc gcagaggcgg atgaacgtcg ccagctgacc ggctttcttg ccttcgccgt
    16081 cgatactgtc gaaaatctcg ttgacacaga ggctataatt cataagtggc cacgttccct
    16141 tcgctttcct ggaccgtcac tttgacacaa tggggaacgg tgtcgcagat ccacttggcg
    16201 atattttccg ccgtcggatt gaagtcgaag acatcgttca gattctgatg gtccatcttg
    16261 gcgtggacca tttccttgat tttcttgaaa tcgacgacca tgccgtttcg gtccagtgtt
    16321 tcggcctggc aggtcacaga gatgatccag ttatgaccgt ggagatgctc gcacttgctc
    16381 tcataatcga ggaccaggta atgggcagcg gatatttcca tccgcttggt tacagtatac
    16441 aaaaggcgtt cctcccttgc cgcaaggccg gctataatcc cagctggtag atgcggttgg
    16501 cattgttaaa gaagacgtca tcccaatacg tttcagggat gaaatctttg acaaaggtaa
    16561 tataatcggc aaaattagcc aggggccagt cggtcccgaa catgacccgg tcataagagc
    16621 cgaggtagtt cagccagtcc cggagcatgg aaatatagcc ccgcttatcc cgcaggaagg
    16681 tgaccatgtc gggtatcttc ccttccagga ggcctgacaa gtcgacggcg acgttgtggt
    16741 tcttttccag gacggcaatg gcatcctgca ggaagggatt gccgatgtgg cacatgacga
    16801 actggacatc gggatatttg acggccgctt catcgagggt catgggatga ctgtacttca
    16861 agagggcgtt ggccgtagcc gtcaggcccg tgtggatggc gacgggcttg ttatacttcc
    16921 gggccagctc gtaaatgggg tcatagagct catcgtagac gtagacgtgg ttatagcccg
    16981 gatagagctt gatgccgcag cactgcttgc gcttgaggtt ctcttcgacc tgggccgtca
    17041 tgaggtccat atcgtcgtaa aagacgttgc tgtccaggcc gatgcagtag ctcatgtaat
    17101 cggggtagtg atggcctgcc gggtccaggg tcttgttgcc catgacgacg ccgtggacga
    17161 tgttgttgtc ctgaaaggcc ttgtgcaggc attcttccgt attagtatag cccgcagcca
    17221 gggcaatctg gttgaagccg acgtaatcac aaaaatggaa gtggctgtca atgacccgta
    17281 gttctttagc tctcataggc ggttactcct ggatagccgt ttccaaggca atgcggatca
    17341 tatcgtcgaa ggacgtctgc cgttcttccg gcgtgcaggc ttcgcccgtc agcaagtggt
    17401 cgctgaccgt gaacaatccc agggcctgag cgccgtattt ggcagccagc gtatagaggc
    17461 cggccgtttc catttcgacg gccatgatgc cgtactgggt cagtttatca ttgtcgattt
    17521 cgtcatcgta gaagcggtcg acagagacga tgttgccgac ccgcgccggg atgccctgtt
    17581 tgaccgactg ttcgtaggcc tggcgcagga gcgtgaaatc agccagtggc gcgtagttga
    17641 tggagccgcc gaagatgttg cggatgatcg acgagtccgt cgtggccccc tgggcgatga
    17701 tgacgtcgcg gagcttgata tctttgcgca tggccccgca ggtgccgacg cggatgagct
    17761 tcttcacgcc gtaatcgcgc atgagttccg tcgcataaat ggaaatggac gggatgccca
    17821 tgcccgtgcc ctggacggac aggcggacgc ctttataggt ccccgtatag ccgaggatgt
    17881 tgcggatatc cgtgtagcag acggcatcgt ccaaaaaagt ttcagcaata tatttagccc
    17941 gcaggggatc gccgggaagc aagatgcggt cggcgatttc accgtcacga gcagcaatat
    18001 gaagactcat gataaattcc tccttaaaaa tagcatatct gtattatacc acatggtttg
    18061 aaaatggcta tctctatgct acaatcccga gtttgacact tttgaaacta aaaaatgctc
    18121 acaaagagcg tggttgtgcg ctctgtgagc attttttatt tgg
//
LOCUS       DAWABZ010000023        18080 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_846_length_18080_cov_3.004161, whole genome shotgun sequence.
ACCESSION   DAWABZ010000023 DAWABZ010000000
VERSION     DAWABZ010000023.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 18080)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18080)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18080
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_846_length_18080_cov_3.004161"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    252..261
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    708..717
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6612..6621
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13893..13902
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17768..17777
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4398 a         5140 c         4881 g         3611 t
ORIGIN      
        1 ggcagcgatg gcgaaagcca gccgctccat gggcaagcca caggcggcac acgatattgc
       61 cgaactggcc ttgtcgattg cccgcaaacg tcgtccgtaa gaggaaaaga agggtgtgga
      121 ataaacctat ggttaatctg aacgatgtta ccaacgttca ttttatcggc attgccggcg
      181 ctgggatgcg ggcaattgcc aatgttttca ttgaaaaagg atatcatgtg tccggttcgg
      241 acctcaaagc gnnnnnnnnn ncggtaacgg aacagttcgc caaaaaaggg gcccgtatct
      301 gtatcggcca gcgtccggaa aacctgcagg acgcccaggt cgtcgtcatt tcatcggcta
      361 tccgcgatac caatgtggaa ctggccgaag cgcgccgccg cggtattcct gtcatccatc
      421 gctcggatgc cctggtccat atcatgagct gggggaaggg gatttccgta gccggtgccc
      481 acggcaagac gacgacatcg tccatgctgg gccaggtctt tgaagaatgt catcaggacc
      541 cgaccatcgt catcggcggc gaagtcgatt acctgcatag caattccatc ctggggaaag
      601 gcgcctatgt catcgccgaa gctgatgaaa gcgatgggac tttcctcaag cagaaccctt
      661 atatcgccgt cgtcaccaat atcgacgccg accatatgga ccattacnnn nnnnnnngat
      721 ccgcgccctg gccccgcaga cgggccgccg ctacgtatct tacggcatcg aacagccggc
      781 ggactatcag gccagcgacc tgcattacct caagggcaag ctccattttt ccgtctataa
      841 gcacggcgaa aagatggggg atatggtcct caacgtaccg ggacgccaca acgtcctcaa
      901 cgccctggcg acgacagccg tcgccgatgc ctgcggcctg cccttcgacg gcatcgcgtc
      961 ggccatgagc catttccacg gcgccaaacg gcgtttccag accaagggct tcgtcggtga
     1021 cgtctgggtc gtcgacgact atgcccacca tccgacggaa atcaatgcga ccttgacggc
     1081 tgcccgcgat atggggacgc accgcgtcgt ctgcctcttc cagccgcacc gctacacgcg
     1141 gacgaagctg ctcctcgacc agtacggcag cgcctttgcc aaagccgata aactcatcgt
     1201 caccgacgtc tacagtgccg gtgaagaccc catccccggt gtatcagggg aactcattgc
     1261 caagaaggtc aaggaaacga cgggacaaga tgtcacctat atcccgcaca aagaagactt
     1321 agtggcttat ttaaaggaaa attcccggcc tctcgacctg gtcatcacca tgggggccgg
     1381 agacatttat aaagtcggcg aagcctatct cgctgaagct aaagaaatgg agaaataagg
     1441 aaatggaaga catgaaagat aaaaaaattg ccatcgtcgt cggcggtcct tcgacagaag
     1501 cagaagtatc gcgccgcact ggggctgcca ttgctgacgc tttgacgaaa aagggatatc
     1561 acatcgaaac gatcgaactc gtaccgaaag aattcgccaa gaccttgacc gaaaagggta
     1621 tcgacatcgt cttcaacgcc cttcacggcc gctacggcga agacggtgtc atccagggct
     1681 tgtgtgaaat gatggggatc ccctataccg gtcccggcgt catggccagt gctgttggca
     1741 tgaacaaggt catgagcaag gccgctttcc agggggccgg cattgctaca gcgccctttg
     1801 cctactattt cgccactcag gaccgcgaag ccatcgctgc cgacattgaa cagcgcttcg
     1861 acctgcctgt cgtcatcaag tcgtccggtc agggctcgag tatcggcacg gtcattgtcc
     1921 agaccaagga ggaactccgt ccggccctgg aagaagcctt caagtacagc gcttcgctgg
     1981 tagccgaagc cttcatcgat ggcgatgaat tcaccgtcgc cgtcatggac gacgtcgcct
     2041 tcccggtcat ccagatcgtg tccagcacgg gcaagtacga cttctattcc aagtatgccc
     2101 ctggcgtcgc caagcatctc tgcccggctc cgatttcgcc ggaactgacc aaagaaatgc
     2161 aggacctggc cctgaatgtc ttccatctgt gccactgcag cggcgtatcc cgcgtcgata
     2221 tcatgaccga caaagaaggc catccgttcg tcctggaaat caacaccgtc ccgggcatga
     2281 cggcgaccag cctggtcccc gatgccgccc gggccatggg aatgtccttt gaagatttgt
     2341 gtgaaaagat tttgctggaa gcatcggtag gtaagtttta atgggaaaga aatcatatca
     2401 tacggggcag cgacgggtcg tgctgcctaa cgattctcat gatgtgtttg ttcctcctca
     2461 tcttgagaac gacccgctga acgaaccgga ccgggaagcc ctggccgcca agaaagaagc
     2521 ccagcgccgc cgcaaggccc gccaggcagc acggctgaag aagaaaaagg ctcggcagaa
     2581 acagatttgg gcccggcgcc gccgtatcgg ctgtatcttt acggtcctcg gcgtcttcgt
     2641 tatctggctc ttcctgcgcc tggcgccggt ctctttcggg acgatcatcg tcgatggcaa
     2701 cgagaacatg tcctttgaag acgtctaccg ggcctgcggg gcttattcct atgtcaacgt
     2761 cgtccagttg tcgacagacg atgtggaaga acggttgaag aaagacctgc gcattgccga
     2821 cgcgacagtg acgcgggaat tcccggcgac gatccacgtc accctgacag aacgcaagcc
     2881 agccgctgtc atcacgacgc tatacggctt cgcctatgtc gacaagacgg ggcgggtcat
     2941 cgaactgggg ccgcagatca agggtgtatc cctgccaatc atgacgggca agaaagtcga
     3001 taacctgctc ttaggcgata cggtgacaga tccgaccctc cattcggcca tggtctatct
     3061 ccagagcctg ccgccggata ttctcaagaa tatcgctgaa atcaacgtgg gaaatcccga
     3121 aagcatcgtc gcctatacga ccgattccat ccctatccac ctgggcagcg gcgatgaacc
     3181 ggcagaacgg gcgaagctga cggaaacgct gctggccgaa gttcaggaaa atcatctggc
     3241 cgtccaatac atcgacacgg atgtgcgctc gccccttgtc aagacgaaat aaggcgcata
     3301 gggccttgat tttcaaaaac aggtagataa attttctaag atagtgtaaa ataataagga
     3361 atcctactgc acagggtgtg cgttatagag tctgaaggag gaaaatgcga tatggcagag
     3421 tttgcaaaca taaaagtaat aggtgtcggt ggcggcggta acaacgctgt caaccgtatg
     3481 atagaaagcg gcttgcaggg cgtccagttc atttcggtca atacggaaga ccaggtcctg
     3541 gaagtatcca aagccgatgt caagatccag atcggtgaaa aattgacgcg cggcctcggc
     3601 gccggtgcca atccccaggt cggcgaacag gctgccctgg aaagcaaaga agaaatcgtc
     3661 aaggccctgc agggtgccga catggtcttc gtcacagccg gcatgggcgg cggtaccggt
     3721 accggtgcag ctcctatcgt tgccgaatgt gccaaggaaa tgggggccct cacggtggct
     3781 gtcgttacca agccgttctc ttttgaaggg aaacgccgta aggaagtcgc tgataaaggt
     3841 gccgactact tgaaagaaaa ggtcgacacg atcatcacta tccctaacga taaattgctc
     3901 cagatcatcg acaagaagac gcccctcaag gacgcattcc tcgtcgctga cgatgtcctc
     3961 cgccagggcg tacagggcat ttccgatttg attacgacga caggcctcat caacctcgac
     4021 ttcgccgatg tcaagaccat catgtccgac cagggcgaag ctatcatggg tatcggcgtc
     4081 ggctccggtg aaaaccgtgc tgtcgaagct gtcgatggag ctatccacag cgccctcctg
     4141 gaaacgagca tcgacggtgc ccagagcatc ttgctcaacg ttaccggcgg cccggatatc
     4201 agcttgtacg aaatcaacga agcagctgaa aaagtggctg aagctgtcga tccggaagcc
     4261 aacatcatct ttggttccgt cattgatccg gatatggaag attccatccg catcactgtc
     4321 gtggctacgg gttttggcaa agaaccgtct tcgattcccg catttggcca gggcactggt
     4381 acagcagcca aagccggcga tacgaaagac aatgccggcg gcgtagaaat tcctacgtgg
     4441 atgagataat cagcatgaaa aaagcggatc ccggcggacc cgcttttttt taaaaatacc
     4501 gtcgagaaga aggtaggagg aaaaaacatg agatgcccat tttgccaatg tgcagataca
     4561 aaagtaacgg attcgcgggc taccgatgac ggcaaggcca tccgtcgccg acgcgaatgc
     4621 cagcagtgcg gccgccgttt cacgacgtat gaaatggtcg aagaagtgcc gctcgtcgtc
     4681 gtcaagaaag atggccgtca tgaattattc gaccgcaaca aattattgaa cggcctgctc
     4741 cgggcctgca acaagcggga agtcacgcgg cagcagctgg aggacatcgt caccaaggtc
     4801 gagcgtaaca tccgcaatac cctggcccag gaagtgtcca gtgaaaagat aggggaaatg
     4861 gtcctcagcg agctgcgctc tgtcgatgaa gtcgcttata tccgctttgc ttccgtatac
     4921 cgcgaattcg ccgatctgga cagtttcatg gctgaactgc agcatctcat gggcacgaaa
     4981 gaaaagaaat aagagaccgg aatcgaggaa cattatgaca gaaaattata tcgatcattt
     5041 tttgaaccat gtcctgccgg aattgcgcat cgccgtcatc ggcgacatca tggtcgaccg
     5101 ctatgtgttc ggtaaagtgg aacgcatttc gccggaagcg cccgtacctg tcaataaagt
     5161 cgaccacatg acgtccgtcc tgggcggggc tgccaacgta gcctcgaacc tggccaatct
     5221 cgattgtcac gtctttctgg gcggcctcat cggcgacgat gaaaacgcgt ctctcctgca
     5281 ccagctgctg gaagacgccc atatcgacgg gtcgggcctg gtcgtgcgcc aggaccacgc
     5341 gacgacgacg aaaatgcgca tcctcggcgc ccgccagcag atggtacgcc tggattttga
     5401 agaagtgacg cccttggccg ttacggaaga ggaacaactc atgacctggc tgtacaactg
     5461 cgtggcagcc ggcctggacg gcatcatcct gtcggactat aagaaaggcg tcctcaccga
     5521 ttccctggct cagagcatca tcaaagaagc caatcgggcc gggattcccg tcctggtcga
     5581 ccccaagggc agcgattgga cgaaatacaa cggcgccgat ttcgtgacgc cgaacatgaa
     5641 agaactgagc gactgcctgg gacggcctgt agccaatgaa ggacaggccg tcgtagcggc
     5701 agcggaaatc ctgcatgagc agtatcagtt cgcccacctc atggtgaccc gctcggaaaa
     5761 gggcatcacc gtcatcggcc gggacggcaa ggtgtggaac aacccggcga cggcgcagga
     5821 agtcttcgac gtatccggtg ccggtgatac cgtagccgca gctttcctgt cggctattgc
     5881 cggccacttg tcgattcgca cgagcctgca gatcgccaac gccgcagcgg gtatcgtcgt
     5941 caccaaggtc gggacctatc ccatccatcg ccacgaattg ctcaaattat ggcaggaatg
     6001 gcagccggcc cactggcatc cgtatcagcc cctgagtcgg gaagaagcgg ctgaaaagat
     6061 acgccaatgg cagaagaagg gcgataccgt cgtctttacc aatggctgct tcgacatcct
     6121 gcaccgcggc cacgtcctct acctgcagca ggcggccatg ctgggccagc acctcgtcgt
     6181 cggcctcaac tccgatgcgt ccgtgcggcg cctgaaaggg gaaacgcggc ccctggtcaa
     6241 acaggaagac cgggccatcc ttttgagtgc cctggcctgc gtcgatgaag tggtcatctt
     6301 cgaggaagat acaccgaaag aacttttaca ggtcctgcgt ccggatatcc tggtcaaagg
     6361 cggcgattac aaagctgatg aagtcgtcgg ccgggaatcg gtcaagcgcg tcgagatcat
     6421 ttcctttgaa gacggatatt cgacgacagg cctgatccag aaaatagctg atttagtaaa
     6481 gaaggggaaa ctatgaaaat tgtaacaggc ggtgccggtt ttatcggcag caatatcgta
     6541 aaacagttga acaaccgggg catcaacgac atcctcatcg tcgacgacct gacggacggc
     6601 cgcaaatgcc gnnnnnnnnn nccagggcct cgatttcttt gattacatgg actatgccga
     6661 cttcgacgaa cagatggccg acgacacctt cgactgcggc tacatcgacg tcgtcttcca
     6721 tgaaggggcc tgctcggaca cgatgaacta cgacggccgc tacatgatga agaacaacta
     6781 tgaaggcagc aagaatctcc tgcgctactg catgcagcgc aagattccct tcctctacgc
     6841 ttcgtcggct tcgacttatg gcagcggcaa gcacggcttc cgcgaagacc cggcctgcga
     6901 acaggcgctg aatccctatg cctattccaa gctccagttc gaccgcttcg tccgccgcgt
     6961 catgcccctg gccaagagcc agattgtcgg cttccgctat ttcaatgtct tcggtcccca
     7021 ggaaaaccac aaggaccgca tggcttcgct catctaccag aagtaccatg aactccagga
     7081 aaaagggaag attaccctct ttgaagggac ggcaggctat gaaaatggcg gccagatccg
     7141 cgatttcatc tacgtcaacg acgtagtcaa cgtcatcttc tatttctggg aacatccgga
     7201 actgtcaggc atctacaact gcggcaccgg catcggccat acgttcaatg aattcgtcca
     7261 gggcgtcatc gattattgcg gcaaaggcca tatcgaatac gtccccttcc cggacatcct
     7321 caagggcaag taccagagtt atacccaggc cgacacgacg aagctcatgg aagccggcta
     7381 tgaccgcggc tttaccctgc tcccggaagc cgtcaaggaa tactgcagca tcctggaaaa
     7441 gagcgacggg tattacgaat gaggaaggcc gtctttttcg accgcgacgg cgtcctcaac
     7501 gtcgacaaat cctatttggg cttcatcaag gattttgaat ggatggacgg ggccaaagag
     7561 gccctggcct atctgacgga agagggctat gccatcatcg tcgtcaccaa ccagagcggc
     7621 gtcgcccgcg gttattatac ggaagacgac gtgaaggtcc tccacgactg gatgtgccgg
     7681 gaagcggcca aagccggcgg catcattacg gccgtctact actgccctta tcttgaaggg
     7741 gcgccggtca aggcctatga caagaagagc gactggcgca agccggcgcc gggcatggtc
     7801 ctgcaggcgg cgaaagatta cgatatcgat ttgaaacact ctttcatgat cggcgatatg
     7861 ccccgcgatg tggaatgtgg gcagcgggcc ggcatggatg gctatttatt tactggcggc
     7921 aggctggacg atttcgtccg ggccatcgtc gcagaaagga agggccgtga aagcatataa
     7981 gaacatactg gtcatcaaga tgagctctct cggcgacgtc cttcacgccc tgccgacact
     8041 ctacgcattg cgtcagaact gcccggacgc ccgcatcgtc tgggccgtcc atgaacagtt
     8101 cgcgccggtc ctgccgggaa aaccttatat cgatgaagtc atctatatcg acaagaagcg
     8161 actgaaatcc ctgtcgtact ggaaagagct gcgccggacg ctccatgcct atcatttcga
     8221 cgtatccttt gacctccagg gcctggccaa gagcgctatc gtcgcctggt tgagcggtgc
     8281 tgaagaacgc tatggctact gggaaatgcg cgaaggcagt ttcctggtca gcaagggcct
     8341 cacgggtccc cacaagtacg accacgtcat cgaacgctat ctcgatacgg tccgcgtcct
     8401 cggcggccgc gtcgatgcca tcgaattccc gcttcccgat attaccggtg aaagacgggc
     8461 ctggcgccag cgtctggcgg cggacggcct gaccggaccc tatgtggcca tcgtgcccgg
     8521 tgcccgctgg agcgtcaagg aatggccctt gtcccattgg caggaattcc tgcagcgcct
     8581 gacggcgtcc ggcatggtcg ccgtcctctt aggcagcggc gacgacgtcc ccaaagggaa
     8641 ggccctgaag gaccatgatt cgtcgggcca ggtcttcgac tatacaggcc gcaccgattt
     8701 gcggcagctc atggcggcca ttgctgaaag cgccctctat atcagtgccg atacggggcc
     8761 gctgcacatg gccaatgcct tgaaaaaaga actcatcgcc ctctttgggc cgacgtgtcc
     8821 ccagcggacc ggcccttacg gcggaaacga cgacgcctac atccacatgg tcctgtcgcc
     8881 gacgtcccag gcgacgccgg aacggccctt ggtcgatgat ccggactgca tggcccagat
     8941 tacgcccgat gcgctgtggc aggtctatga aggcgtcctg aagaaggtgg aactatgatt
     9001 aatctgcgca acaaaaaaat aatcgtcacc tttctcatgc atctaggcga cctcatcctc
     9061 atcaccccgt tcctgcaggt cctgcgccgt catgcccagg gctcggaaat taccctcgtc
     9121 gtcgatgaga aagtggccga cgtcgtccgc tacaacccca atatcgacca cctggtcacg
     9181 gtcgacaaga agggtaaaga caattccgtc cgggccctgt ggcgtatcgg gcggcacttg
     9241 catcagcatc actacgatat cctcatcaat ctgcatccca atgaacggac gtccttcctg
     9301 gcggccgtca tccacgccaa gcagttcgtc ggcatgagcc atttcctggt ccggcccctg
     9361 atggaccgct atacccgcct ggaccgcatc catctccacg cggccgatat gtacatcaac
     9421 gtcctggccc agttgggcat cgatgactac cgcagcgacg gcctgcagtt cttcacctgc
     9481 aaggcctggg atgacaaggc gacggaattc taccgtaccc agggcgtcca caacagcgat
     9541 gccctcatcg gcttcaatat cggcagcgcc gttccgcaga aacgctggcc ggccaagcgc
     9601 tttgccgccg ttgccgacta cttcgcccgc cgcggcttca agtgcgtctt cttcggcggc
     9661 cctatggatg aagccatggt caaagaagcg acgtcccaga tggaatcgga cccgatcatt
     9721 gccaccggga aattcaccat cggcgaactg gcctcggcca tccgctgctg ctcgctgttc
     9781 atcaccaacg acagcggccc tatgcacgtc gccgtcagtc agggcgttcc cctggtcgcc
     9841 ctctacgggc cgagcaatcc caagctttac ggcccctata cggaccgggc catcgtcctg
     9901 gaatcgacga accactatga agttggcaag agcatgaaac aaattatccg cgaaggtaat
     9961 tataaaggta tttcggttat tcccatgagc caggtcatcg ccgccggcga agaactcctg
    10021 agccgttatt atggaagaaa ataacccgta ttatatggag gtggaaggtt atgaaaaaac
    10081 ttattattgc agcgaccctg gccgccctgg ccatgacttc ggctgctatg gcagccccgt
    10141 tgcgggatcc tcagcccggt gacttgaagg ccaacgccaa ctacggcttc gaccagaaag
    10201 aaggcggccg cagtgccaag agccgcctga ccggtggcga cgtcacctac gtcctgaaca
    10261 atcgctggga catccagtac gtcaacaact acaccaaggg cgataacgac aacaagatca
    10321 acgaaaacta tctcgtcggt aattaccgct ttacgccgta tctgtcggcc tttgccggcg
    10381 gctcctacgt caagaccgaa acgtataata cgaccaagtc ctatggctat caggtcggcc
    10441 tcaaaggcca gctccctctg gcagcacgct ggcagggctt tgcatccgtc ggtgtcggcg
    10501 atgacgtcaa tacctatgaa gtcggcgtcg gttacgacat cacgccgaac tgggatgccc
    10561 acataaaata ccgcagcagc atcgtcgacg tcgataacta cgacgacgat gtcaaaggct
    10621 ggcaggtcgg catgggctat aagttctgat attgacaagg ggcggatccg tcccgtataa
    10681 tgtaccaatg acatgtgctg tggttgaaag tcgatgtcag tcgcagacca agcgatccac
    10741 gtaaggcacc caaagcggtg ctgatcatag tgcggcctag aagtaagacc tgccgcggaa
    10801 ataccgcgag agggctagta gtgggggaca gcagtctatg agcgaacgtc ccagcaggcg
    10861 agtgtggggg taaagaccag gtcaggcatg tcagggaaag gggctgtcgt atgaaggatt
    10921 ctgccatcat gcgcagtccc tttttcttta tagtttgtag tttatagaag atggccataa
    10981 atttcaaacc atccgctaac cactagcccc tagcccctag ccccgtaaaa gaggctgtct
    11041 tgatgtgacg gcatcttttt tgtattttcg tttgacggat cattgttcgt atagtaaaat
    11101 gtaagggtat ttatgttatt agcctaagga gtggagtcat aatggatgct aagcggttgg
    11161 aacgagaaat tcacaaaaat cccttcgata cctttatgga tttccacgtc gaaaaggctg
    11221 aaccagagag tacgatcctc acttttgaga ataaaggctc gaaatgggat aatcccaatg
    11281 gtaccatgta cggcggtgtc ctctattcca tggccgattc ggcgatggag gctgtctgtg
    11341 gcaaagccgt cctcacactg gacctgggta tgaattacct gcgtccggct tttgccaata
    11401 cggtcatccg ggccgaagcc aggatcatcc ataacggccg tacgacgatg gtcgccctct
    11461 gtgattttta cgataatgac cagcgctatt tagctcatgg caagggcacc ttctttgtga
    11521 caggggccta cgtctttgag ggagaaaggg ggcatacgga tgaatgaacc agaagaagca
    11581 gtgagcccgg aagtggcaga acggctgcat cgtatgctgg acagtattta ttcgcataca
    11641 ccttatttcc agagcatgcc ctgtacggta gaaaagctcg acgtcggccg cgtccgcatc
    11701 ggcttcgata tcagccgtca ttcctgcaat taccggggca ttgcctcggg cggcgtcctg
    11761 gccgctttct gcgataccct catgggcatg gccagccgga ccctgggctt ccaggtgacg
    11821 acgctggaaa tcaacatgaa ctatatccgc agtgtcaaag gcggcgagca cattaccggc
    11881 gtgggccagg tcattcacca cggccggaag accattgtcg tcgaatgtga atgttatgat
    11941 gccagcggta atattatcgt aaaagggcgg tcgtcttttt atattttgaa aaactcgatt
    12001 gataggataa tcatgtaatt atagggaagg ttggttagta caaagatgga ttttgcttat
    12061 aacgacgaac agaaagaaat catcaaggtt gtccgcgacc tggtggaaaa agaaatcgtc
    12121 ccctacgctt cggaaatgga tgaaacggga aaactccatg aagggctcct ggaaaaattg
    12181 gcagcccagg gcctgctggg cgtcgccatt ccggaagaat tcggcggtgc cggcctcgac
    12241 gccatcacta tcgccgctat ttatgaagaa ttaggcaaag gctgtgccgg cgtcgctacg
    12301 accgtcgctg ccaatgccct ggcctcgtat cccgtcatca ttgccggtaa cgacgaccag
    12361 aagaaacgct atttcgatat catcaatgaa gacaacctgg ccgcttttgc cctgactgaa
    12421 ccgggcaccg gttccgatgc cggttccgtt tcgacgcggg ccatgaagac cgaagacggc
    12481 aaaggctata tcctcaatgg tacgaaatgc ttcattacca acggcgccct ggccgaagtc
    12541 ttcgtcgtct tcgccaacac ccgcaagagc agcggcatcc gcggcttgac ggccttcatc
    12601 gtccgcaagg ggacgccggg cttcacagtc ggcaaggctg aaaataaaat gggcatccgc
    12661 gcttccaata cgacggagct catcttccag gactgcttcg tccctgtcga agaccgtctc
    12721 ggccgcgaag gccatggctt ccgcatcgcc atggatactt tggatgcagc ccgtcccttc
    12781 gtcggtgccg tttccgtcgg cattgccgaa gccgctttcc gtgcctgctg cgaatatgcc
    12841 aaggtccgcg tacagttcgg caaacccatc gcttcctttg aaatggtcca ggccatgatt
    12901 gccgatatgg ccatgaaagt cgaaggagcc cgcctcctgg tccaccgcgc ctgctggatg
    12961 aaagaccagg gtatggactt cagccgtgaa gcggccattg ccaagtgcaa cgcgtccgac
    13021 gtggccatgc aggtcacgac cgatgcagtc caggtcatgg gcggctatgg ctatatcaag
    13081 gaataccctg tcgaaaaata catgcgtgac gccaaaatca tgcagattta cgaaggcacc
    13141 aaccaaattc agcggttggt tattgcaaat aagacacttt actgatacaa tatataacaa
    13201 acccatagag ggatttatgg caatgataaa gaggggatgg aactgactcc gcctacaggg
    13261 aatcgaggcc gcagattgag agcctcgtgc cggatgttcc atgcgttcac tttagagcct
    13321 cctgctgaac ggagtaggca ggacgggggt tcccgttaca ggacaatcga gaatcaagta
    13381 gggtggtacc gcgaatgaga ctttcgcccc ttagggggcg ggagtctatt tttttttgta
    13441 cttgccagta ggaggaagaa agtatgatca gtgataagat cgaggccatc aaagcggccg
    13501 tcaatgaaca gctcgccaag agcgaacatc ttcaggacgt acaggatatc cgcgtcaagt
    13561 tcctgggtaa aaaaggggaa ctgacggcaa tcatgaagga aatgaagaat ttttccaaag
    13621 aagaacggcc taaagtcggc cagctcgtca acacggcccg caatgccatc gaagaacagc
    13681 tcaaggccaa gatggaagaa gtcaaggcca aggaattggc tgcgaagatc gaatcggaaa
    13741 aaatcgacat taccctgccg ggccgcaaac cggtcatggg ccacgaacat ccccttcatc
    13801 agactctgcg cctgatggaa gactcgttcc tgcgcatggg cttctccatc gtcgaaggga
    13861 cggatatcga atcggattac tataacttcc aannnnnnnn nnatctgccg gaagaccatc
    13921 cggcccggga catgcaggac tccatgtaca ttacggataa tatcctcctg cggacccata
    13981 catcgggcat gcaggcacgg accttgcagg cccacaagcc taacgaaccc ttcaagatca
    14041 tcgcaccggg caaggtatac cgctgcgact acgatgcgac ccattcgccg gtcttccacc
    14101 agatggaagg catgatcatc gacaaggaca tccgcttctc cgacctcaag ggcatgctcg
    14161 aagacttcct gcgcgacatc ttcggaccgc agacgaaagt ccgcttccgc gccagctact
    14221 tccccttcac ggaaccgagc gccgaagtcg atatttcctg cgtcatgtgc ggcggcaagg
    14281 gctgccgtgt ctgctcccac acgggctggc tggaaatcct cggctgcggc atggtcaatc
    14341 ccagcgtcct gcgcctgaac ggctatgacc cggacgtcgt cacgggtttt gccttcggca
    14401 tgggcgtcga acgcattgcc atgttgaaat acggcatcga tgatttgcgc ttgttctatg
    14461 aaaacgatat gcggttcttg aaccagttct aggagggtat gaataatgaa aagttcttta
    14521 acttggatgc aggattatgt cgacgtcgac atgaaccagg acatgcaggc atttgccgat
    14581 aaattgacca ttgccggtat ccctgtcgaa caggtggaat actgggggac agaagtcaag
    14641 aaagtaaaga ccgggaagat ccttcagatc gacaagcacc ccgatgcgga taaactcgtc
    14701 gtctgccagg tagacatggg ggatgaacag ctccagatcg tcacggctgc gaccaacgtc
    14761 cgcgtcggcc agatcgtccc cgtcgccgtc cacggcgctc atctgccggg cggcactaag
    14821 atcaagcgct cgaagctgcg cggcgtcttg tccaacggca tgttctgctc ggcccacgaa
    14881 ttcggcttcg acgacagcct gctcttgccg gaagaacggg aaggcatttg gatcctgccg
    14941 cccgatacgc ccctcggcgt cgatgcggcc gactatctcc aggtccgcga cgtcgtctat
    15001 gaatatgaac tgacggccaa ccgcgccgac tgcttctcca tggtcggcct gtcccgtgaa
    15061 ttcgccgtcc tcagcggcaa agaagcccgt tacccggata tttccgtcaa agaatgtgac
    15121 acgcccatcg acggcaaggt caaagtatcc attgacgacg aagaactgtg cagccgctat
    15181 gccgcccgcc tgctcctgaa cgtcaagatc ggcaagtcgc ctatgtggat gcagcagcgc
    15241 ctgcgcaagt ccggcgtccg tcccatcaac aacgtcgtcg acgctaccaa ctacaccatg
    15301 atcgaactgg gcatcccact ccatgcctat gactacgata aagtcgccgg ccatcacctc
    15361 atcgcccgcc gggccaaagc cgatgaagcc ttgaagactc tggatgatgt agaccgtaaa
    15421 ttgaccgacc acatgctggt catcgccgat gaagaaaaag cctgctgcgt cgccggcatc
    15481 atgggcggca tggatacgga agtcacggac cagacgacgt cggtcatcct cgaatgtgct
    15541 tccttcaaag gctccaacat ccgtcatacg ggccgtatgc tcggcctgcg ttccgaagct
    15601 tccggccgtt ttgaacgagg actcgattcg gacagctgca tcaaatccat cgaccgctgt
    15661 gcccagctcc tccaggaaat gggcgcctgc aacgtagccc aaggcgtcgt cgatgtctat
    15721 ccgaaaccgc aggctacgac gcgcatcgcc tttacggcgg ctcaggtcaa caaattcctg
    15781 ggcacggaaa tcccggaagc cgacatggtc catatcctgg aaacgctgca gttcggcatt
    15841 gaaaaagacg gcgatacctt gacggccgtc gttcccagct atcgcggcga ctgcacggaa
    15901 atgccggaca tcgccgaaga agtagcccgc gtctacggct atgaaaacat cccgtctacg
    15961 cgcccggccg ctcccatttc caaaggcgaa gccggcttcc accacgacgt aggcgaaaag
    16021 atttcgtcca tcctcagcag cagcggcctc aacgaaacgg tcacgttcag cttcatgcac
    16081 ccggaccagc tgaagaagct cctcttcaag gacggcgatg ccgtatacaa cgccgtgccc
    16141 atcctcaacc cgattacgga agacttcccg ctcatgcgga cgacgctggt accgaccttg
    16201 atggatgtcc tggtccgcaa ccaggccgtc aagaatccgt ctgtcggcat ctacgaaatc
    16261 gcaccggtct atcggccgaa acagctgccc ctgacggaac tgccggaaca ggaattctat
    16321 gtcagcggcc tcctctacgg ccagcgcacg cctgctgaat ggccggccaa agcggaaaat
    16381 tatgacttct atgacgtcaa gggcctcgtc gaagccgtcc tccagggact gggcatccag
    16441 gccgacctgg aagtctccga ctgggcgctg ctccatccgg gcaaggctgc ccgctacgtc
    16501 aaagacggca aagtcctctg cgacttcggc gaactccacc ccaaggctat cgacaattac
    16561 gatgtcgccg gcccggtcta tgtctttgaa atgcatatgg acgctatcct gcccttggtc
    16621 aacctcctgc cggattacca taaagtcgcc aagttcccgg catcgagccg cgacctggcc
    16681 ttcctggcac cgctggctac ggccaatgcc gacatcgtca gcgtcatcaa agaagacggc
    16741 ggcgacaacc tggaagccgt ccacctcttc gacatgtaca cgggcaaaca ggtaccgcag
    16801 ggctacaaga gcctggccta ttccttagtc ttccgttccg aagaagggac cctgaccgac
    16861 gccgacatcc aggccccggt cgacgccatc gtcaaagacc tgaaagaaaa attcggctgc
    16921 gaattgcggt agggagtgat tggtttgtaa tacaaagttt gtagtttgta gaagatggcc
    16981 tttaattttt aaccatttca ctgcaaacta aaaactacaa acttcaaact caaaaaggcg
    17041 ctgtctttat gcgacagcgc ctttacccat attgtaaaaa aagtaaagtt gtggtatgat
    17101 gtagagcgta atatggtatt ttagagatat tcagggctct accggcccat aaggaggaca
    17161 tacttagaat gaacgtaaca gtgaatgaag tagaccagca caaagtaacc ctgcacattg
    17221 aagtgcctgc taaagacgct tccaaacagg cagcagcagc ttgcaagaac ttggcaagcc
    17281 gtgtcaatat cccgggcttc cgcaagggga aagcaccgcg catggtactg gaaagcttct
    17341 tgggcaaaga tgctgtcaaa caggaagttt ttgaagctat tgccaataaa gcctacagcg
    17401 acgccctcga ccaggaaggc atcgttcctg taactgaacc ggaattgaac gtcattaccg
    17461 acgaaaaagg caaagacgtc gtatttgaag ctacggtcac caagaaaccg gaagtcaaac
    17521 tcggcgaata caagggcatt accgttgcca aagacaaagt cgacgtaacc gacgaagacg
    17581 tagccaaaga actggctaac ctccagaaac agcatgcgaa actcgtcgta gctccggaag
    17641 gcacggaaat tgcaaaagat gacttcgctg tcatcgattt caaaggcact gtcgacggcg
    17701 tcgcattcga aggcggcgaa ggcaaatcct atccgctcca gatcggctcc ggcagcttca
    17761 tcccgggnnn nnnnnnngat cagctcatcg gctgcaaagc tggcgacgaa aaagacgtca
    17821 aagtcacctt cccggaagat tactttgaaa aatccctggc tggcaaagaa gctgtcttcg
    17881 ccgttaaagt caacgacgta aaacgcagcg aactcccggc catcgacgat gaattcgcta
    17941 aagaagccgg caaattcgac agcgtcgacg acctcaaagc cgacatccgc aaacgcctcg
    18001 aatcgaaagc atccttccag gctttggaaa aattcaactc tgaagtcctg aagacggctg
    18061 tcaacaatgc cgaagtcgat
//
LOCUS       DAWABZ010000024        17883 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_857_length_17883_cov_3.780850, whole genome shotgun sequence.
ACCESSION   DAWABZ010000024 DAWABZ010000000
VERSION     DAWABZ010000024.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 17883)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17883)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17883
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_857_length_17883_cov_3.780850"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4235 a         5098 c         4779 g         3771 t
ORIGIN      
        1 taccagttgt tcatatcgcc ccttcaccgg ccgtgctgcc ggttttatcc gacttgttct
       61 gcctatgcca tcgaggcttt gcagaagtac ggtgctgtca aagggagtta tttggcacta
      121 aggcgcattt taaaatgcca tccctttcac aaaggcggat atgatccggt accgtagatc
      181 cctattttta caactgaaca ggagttgaga gtattgggct ttttcagtgg aataatggat
      241 gtcctcagcg gcattatgac gacattcatg aattactgtt atgcatttac ccagaatgta
      301 ggctatccga gctatggcgc tgccattatc atgctcacct tgattattaa actggttctg
      361 gctccgctta cggctaaaca gatccgttcc atggaaggca tgcagaccct gcagcctaag
      421 atcaaagaag tacagaagaa atataagggc aatcagaaaa agatgcagga agaaatgacc
      481 aaactgtatc gggaaatggg cgtcaacccc ttgtcgggct gtctgccgat cctcattcag
      541 atgccattcc tcatcgccat tttctatgct cttcgggaat atccctacga cccggccttt
      601 gaaagtttct tatggcttcc cagcctgggc cagccggacc ccacatatat catgccggtg
      661 ctgtcggctg tttcgactta cttcatccag aaccagatgt ccggtacgca ggtagcggct
      721 tcggatgcac aggctaaaca gcagaaaatc atgaagatcg tcatgccgtt attcatcggc
      781 tggatcagct tgaatttccc cagcggcctg gtcatttact gggttgtttc taacgtattc
      841 cagtgggcac agcagttcct catgacccac ctcaagaaaa aaggagcacg atcagaagca
      901 tgagtaccat cgaaactaca ggaaaaacga tcgaagatgc cgtccgctcc ggtctggtcc
      961 gtctcggcag aatgaaggac gaagtcgaca tcgaagtctt ggaagaaccg aagagcggtt
     1021 tcttcggatt cggcagcaag ccggcgaaag tacgcattac cgaaaaagaa ggcgtaccga
     1081 cggtcccgac catggaaagc cagccggcag ccgtggaaaa gcccgtcgca ccggcagcgg
     1141 ctgaaccggc tgccaacgca gctggcgcag ctgaagaagc ggttccccag gtagaaacag
     1201 aaccggctga agccgaaaca ggcgatgatt ttacggctga agaagccgcc gccaaggcga
     1261 aggccttcct tcaggaagtg ctcaagaaca tgggcatcga cgtcgtcatc gaaaagatga
     1321 tcaagtccga taaagtcctg ctccacctgc acggcaagaa cttgggtatc ctcatcggca
     1381 agcatggcca gaccttggat gccctccagt acctgaccaa cttgacgacg aatcagggcg
     1441 aaaggacgcg ccatttcatc atgctcgatg tcgaaaatta ccgccatcgc cgggaagaaa
     1501 cgctgaaaca gctggctatc cgtctggcga accgcgtcaa gaagaacggc gaccgcgtcg
     1561 tcctggaacc gatgaacggt tatgaacgca aaatcatcca tgtgaccttg cagaacgacc
     1621 cgcaggtccg cacggaaagt gaaggccagg acccgtaccg ccacgtcgtc atttactatc
     1681 aagaataatt tttagtttgc cctatgaagc ttaacaaaga ggctgtcgca ttcagacagc
     1741 ctctttcgag tatttttcta caaactctgt actacaaact ataaagtaaa aggaatcttg
     1801 catgatgtca ggaaacttca tgcgagcccc ttggctatga aaggagtatt gccatatgta
     1861 cgatacgacc gatacgatcg cggccattgc cacccccctg ggggaaagcg gcatcggcgt
     1921 catccgtatc agcggcagcc atgcctatga cgtaggcgat gccattttcc gcagcccttc
     1981 gggaaagccc ctggcccagc gccaggaccg ttctatccag catggcttca tcgttgccga
     2041 tgacggccgt cctgtcgatg aagtgctcat gctcatcatg aaaaggccgc actcctatac
     2101 ggcggaagac gtactggaaa tccagtgcca cggcggccgc aaggccttgg aagaaatcct
     2161 cggcctggtc ctggcccacg gcgcccgcat ggccaatccc ggagaattta cgcagcgtgc
     2221 cttcgtcaat ggccgcatcg atctggccca ggccgaagcc gtcatggatg tcatccaggc
     2281 caagagccgc cagggcctga ccagtgccgt caatcagctt gaagggcggc tgtctaaagt
     2341 catcggtgcc atgcgccatg atttgacgga cttcattacc cgcctggaag tcaccatcga
     2401 ttatcctgaa gaagacctgg aagatatcac cgtccccgat gtagccggag ctatccgcaa
     2461 acagctgacg gccctggacg atatgctgga ggcttctcac gacggccgca tcatccgcga
     2521 cggcgtcatg gccgccattg ccggtacgcc gaacgctggc aaatcgagcc tgctcaaccg
     2581 cctgctccag gaagaccggg ccatcgtcac cgatgtgccg gggacgacgc gggatgtcct
     2641 ggaagaatgg atttccctca aaggtgtccc ggtctgcctc gtcgatacgg caggtatccg
     2701 ggaaacagac gataccgtcg aaaagatagg cgtcaccaag gccaaggcct atatggacaa
     2761 agccgatatt atcctggtcg tcatcgaccg gtcccggtcc ttgtccgatg aagataaggc
     2821 catcctggaa gacacgaagg ataagaatgt cctggtcgtc ctcaacaaga ccgatttaat
     2881 gccagtcgtt acggaaaaag atttggcagg ctatggtcat cccgttctct ccttgtcggc
     2941 tggtacgggt gacggcctgg attccctaaa agataaattg ttgtccctgg ccctgtccca
     3001 tgccgatacg gcaggcagtg ccctgctcac caatacgcgc catatcgaac tggtgcgcca
     3061 gagccggcaa gccctgcagc gggccctcga ttccatcgaa gccggtatgc ccgtcgactg
     3121 tgccgtcgtc gacctgcgcg aagcctggga gcttttgggc tcgatcacgg gcgatacggt
     3181 ccacgacgat atggtcgaag aaatattcag ccgattctgt ttaggtaaat aaaggggtgt
     3241 aaccatgttt gtagtcgata catacgatgt catcgtcatc ggtgccggcc atgccggctg
     3301 tgaagcggcc ctggccagtg cccgcctggg cgccaagacc ctgatggcga cgctgaactt
     3361 agataatatt gccctcatgc cctgcaatcc cgctgtcggc ggaccgggga agagccatct
     3421 cgtccgggaa atcgacgccc tgggcggtga aatgggcatc aatacggata agacgtgcct
     3481 ccagatgcgc atgctcaaca ccggcaaagg accggccgtc tattctctcc gggcccagtc
     3541 cgataagaag atgtaccaga tggccatgac caagaccctg gaaaaccagg accacctcga
     3601 tgtcaagcag ctcatgatca cagaccttct cgtcgaagac ggcgccgtca ccggtgtccg
     3661 gacggaactg gatgaagttt ataaagccaa gtgcgtcatc ctggcgacgg ggacctacct
     3721 caaagggaag atcatcatcg gcacctgtac ctattccggc ggccctatcg gccagcgctc
     3781 ggctgaagat ttgtcggggt ccctggtcaa ggccggcctc aagcttatgc gcttcaagac
     3841 cggtacgccg gcccgcgtcg accgccgtac cctgcggacc gatgaaatgg ccaagcagga
     3901 aggcgacaaa gacggtcatg ccttttcctt cctgtcggaa cggaaagacc gcaacaaaga
     3961 atgttgctgg ctgaccttta ccaacgaaga aacgcataag atcatccgcg acaacctcga
     4021 ccgggctccc atgtgcaatg gcatcatcac cggcatcggg ccgcgctact gcccgtccat
     4081 cgaatcgaaa atcgtccgct ttgccgataa gaagcggcat cagctcttca tcgaaccgga
     4141 aggggaatcg acggaagaaa tgtacgtcca gggcatgagc accagtatgc ctgccgacgt
     4201 ccagtacgcc ttcctgcgta ccatcccggg cctggaagac gtcaaaatca tgcgcccggg
     4261 ctatgccatc gaatatgact gcctggaccc gacgcagctc acgccgtggc tggaaacgaa
     4321 gaagatttct ggcctcttct cggcaggcca ggccaacggg accagcggtt acgaagaagc
     4381 cgctgcccag ggtctcatgg ccggcatcaa cgcggccttg aaggtccagg gcagagaacc
     4441 gttcatccta acccgctccg aagcctatat cggcgtcctc atcgacgatt tggtcacaaa
     4501 ggggacgaat gaaccgtacc gcatcatgac cagccgcagc gaataccgcc tcatcctgcg
     4561 tcaggacaat gccgacctgc gcctgaccca gaaaggctat gacctgggcc tggtcacgca
     4621 ggaacggtat gaccgcttca cggccaagaa aaaagccatc gaagaaggca tcgccgccct
     4681 ccaggccatt tccgtcacgc cgtccaaggc caatctggaa aaattggaac gcctgggcac
     4741 ggcacccatc cacacggcca tcacggccta cgacctcctg cgccgcaacg atgtcgacta
     4801 cgaggccctg cggacggtct tcgacctgcc ggaactggct gccgacgtgg aagaagaaat
     4861 cgaaatcatg atcaagtacg aaggctatat cagccgccag atggaacaag tcgaaaagat
     4921 gaaccgcctg gaacagaaga agatgcccgc cgatatcgtc tatgccgata tcgacaccct
     4981 gtcggccgaa gcccggcaga aactcgatga aatcgggccc ttgtccttag gccaggccag
     5041 ccgtatttcc ggtgtgtccc cggcggatat cacggccttg ctcatcgtac tggaacagca
     5101 cagccgggag gaaaaaaatc atgtttcgca gtgaattaga acaggccctg aaggccatgg
     5161 gccttaccgt gacagacggg cagctttccc agttctgcgc ttttaatgaa agactggtgg
     5221 aaacgaataa agtcatgaac ctgacggcca ttaccgaccc gaaaggcgtc gccatcaagc
     5281 acatggccga cagcctgtcc tgttatgacc cggcttattt caaagaaggc gctcgtgtcc
     5341 tcgatttggg gactggcgcc ggattccccg gcctgcccct gctcatctac cggccggact
     5401 tgaacgtgac gttcttcgat tccctgcaaa agcgcctgac cttcctggaa aacacggtca
     5461 gcgatatagg actgacggcg tcgttcctcc acggccgggc cgaagaaatg gcccataagc
     5521 cggaataccg cagcgctttc gatatcgtca ccagccgggc cgtcgcccgc ctgaacatat
     5581 tggccgaatg ggccctgccc tacgtgaaga ccggaggcta tttcatttcc ctcaaagggg
     5641 cccagtatga agacgaactg gcagaaggcg aaaaagcctt gcagatcctg ggcggcaaaa
     5701 tcgccgaagt ccgacctgtt cacctgccgg gactcgatga cgtccgcgcc gtcctttaca
     5761 tcgagaaagt gaaggaaagc cccaaaaagt atccccgcaa gcccaagatg gctgcgaaaa
     5821 atcccttata gagaaagaag tgtatgctat gtctttgaaa agaatcttct gtctggctgc
     5881 agccgcctgc ctgactgtgt cggccgccgt ctttgccgat acgccgcagc cacctgaaat
     5941 cagcgctcat gccgcctatg tcatggatgc cgatacgggt gacacgctct atgcgaaaaa
     6001 tgccgacgac cggacctatc cgggcagtac gacgaaaatc atgaccggta ttctcggcgt
     6061 cgaattgggc aaggcccagg gcaaaatgga ccagcccctc aacatcacca atgacgtcta
     6121 caacatcgaa agcgatgcgt ctgttctggg catcgatcct ggcgaccaga ttaccctgcg
     6181 ccatgccctg accggcatga tgaccgtatc cggctgtgat gcggccgttg ccgtagccga
     6241 aacggttacg ccgacccagg ccgatttcgt cgccaagatg aatgaaaaag cagccgccct
     6301 gggctgcacg aatacccatt tcgccaatcc ccatggcctg ccggacagcg atcactattc
     6361 gacggcccgc gatatggcga cgattgccgc ttatggcatg aaaatgccgg aattccgcga
     6421 catggtcagc cacagtgaat acgacatgcc ctatatcaac ggcgggtcca agcactgcac
     6481 gacgacgaac tatttcctga ccagcggctt ccccggtgcc aacggcatca agaccggcac
     6541 gaccaacgcc ggcggtccct gcctggtcgc ttcggctacc caggatggcc ggacggtcat
     6601 cgcttccatc atgaactcgg acgaccgctt cggcgatgcc cagaaattga tggcctatgg
     6661 tttttccgtc ctgaaaccgg tcgaaccggc agaaaacgtc tatatcctgc gtcaggcacc
     6721 ggcaggccag acattgtcgg atatcaggga aaaaaaggcc caggctgctg aactggcatc
     6781 gcagcagaag caggaagccg ctacggctgc cgcttccacg gctgcggcca aagcgcctgt
     6841 caaaacagct acaacggtaa agaccgcaac ggaaacagct gctaaaacgg cagccaagac
     6901 gactgcgaaa accgctgcca aagctgatac ggcgaagacc agtaagccgg ctgtttccag
     6961 ccagccttac gttcccttct cgccggctgc ttaaaagtta aaaaagggat attgacaatc
     7021 accgtccata ttcgtataat aagcacatca gcagattcgt acaggactga cggaatcaag
     7081 tggagtaacc acatgaactg tacggagatt ttcatgccga ccgtctgggc aagagtatgc
     7141 tacatattct tgagccaggc ggccggtatt ttttttcgcc agtctggctc ctcacaagga
     7201 gtgttgtcta aatgaaacga aatcgctggc tcatcgccct gtctgccatg gggctccacc
     7261 tctgtatcgg cagtgtctac gcctggagcg tcctcaccaa gcccatcatg gaatctatgg
     7321 gcttcaccct caaggaaacg acgtggacct tttccctggc catcctcttc ctcggcctgt
     7381 cggccggctt tttgggcagt ttcgtccaga aaatcggtcc ccgcaagtcc ggcctcttat
     7441 ccatgacctg cttcgtcacg gccatgttcg gcacggcagc ggctttctat gcccacagcc
     7501 tgcccatgct ctacgctttt tacggcgtca tcggcggcgt cggcctgggg acgggctata
     7561 tcacgcccgt atcgacactg gtcaaatggt tcccggaaaa tcggggcttt gctacgggcc
     7621 tggctatcat gggcttcggt tttgccagcc tcatcgccgg tccggccatt cagctgctgg
     7681 tccagcattt tggcttagtc gctaacttca tcatcctcgg ctgcgtctat atggtcgtca
     7741 tgacggcttc gtcgctctac ctggctccgc cggcagccgg agacgttccg gccaaggccg
     7801 aaaaagaatc ttccgtcgaa tacgccgacg cccagccgca gttcaccgtc cgccgggcca
     7861 tgaagacctg gcagttctac gccatctggt ggctcttctt caccaacatc acctgcggta
     7921 tcgccctgct gtccgtcgct tcgcccatgg cacaggatgt cgtcggcatg tcgcctatgg
     7981 cagcggcttc catggtcggc ttcatcggcc tcatgaacgg cgccggccgc attgcctggg
     8041 cgaccatatc cgactacatc ggccgctcgg ctacgtatgt cctcttcttt gtcattgaaa
     8101 tcctggcctt ctggcagctg gctggcgtta cgggcgcctt cagtttccag gtcctggtcc
     8161 tgctcatcat ttcctgctat ggcggcggct tctcgtgcat gccggcctac ctcagcgacc
     8221 tcttcggcac gaaatacctc agtgccatcc acggccgcat cctcacggcc tggggcgctg
     8281 ccggcgttgc cggccccctg gtcctgtcgc tcatcaaaga aaataccaat agttatgccg
     8341 tcaccttgta cttcttcgtc ggcgccttca ccgtcgccct ggccctaatg ctcttcctca
     8401 aataccgcac ccgccacggc atcgcccgcg tcgcagaagc acggggatga tcgttcatcg
     8461 taggcctgat gatcgtttat cgtaggtcgt tcgtcgtagg gggccgcacg cgggcgcccc
     8521 acgggtcgcc cctacaacga aaaaagctgt cgctatgcgg cagctttttt ttcgataacg
     8581 acgaacgatg aacgaatcgc agccaggcga caaataatcc aaggcctgtg gccttgtgtg
     8641 taggggacgt cctgtcaagg cgtcccgttg tgaacaatgg aagatatact taactgatat
     8701 atgtggcatg gttttgcgac ctgcgaccga cggcctgcat tctcttggac tcagatgccg
     8761 ctgtgcggtg acagactcaa actgacaacg acgcttcggg aagccctccg cgcgtagcgg
     8821 gccgccttta caggccggcc cctacggaat cgcagccagg cgatgttatg gatttacgac
     8881 aaacgatgaa cgcattacca taggcgatgt tatggcagaa ccgttatgga ttaacggcaa
     8941 atcattcacg gcctatggcc ttgtgcgtag gggacgctct ttaacctctc agaccgcctt
     9001 cggcggccag ctcccctata aagggagcct tgggcgtccc gttgtgaaca atgggagata
     9061 tactcaactg gtctatgaga aatggttttg tgacctgtga actgcgaata atgatatcga
     9121 cgaacagcat ttcacgggat tcccacaacg ggccgcccag cctctgctgt ccgttccagc
     9181 aaccgtcttg cgcctcccat tggtcggcac ccgacgggct ggaaaggctt tggacggccc
     9241 ctacagcgac atctgtgatg tcgcggcggc ctgcgggctc gccacgtgcg agcccctaca
     9301 tatgtgaata aaatcactta atgagccgga cgcatagatg aacgaaaatg attgcttttt
     9361 gattataatg aatgaatttg attgaaaatg attgaatata acggtataat gaggccaaag
     9421 gaggataaaa agggaagtga tacgatgttg acagaagaaa gacatgaaaa gatcatgcgt
     9481 cttgtcaata cgagtggtgc cgtttcggtt caggacttag tgagttttct gaacatttcc
     9541 gaatcgacgg tccgtcggga tttactcacg ctggaccggg aaggccgcct gcgccgtgtc
     9601 catggcggcg ccacgacgct gcgcgatgat tctttatatg aagcggatat ggaaaatctg
     9661 caggacaagt attccattca tatgagcgat aaacgccgca tcgcccagta tgcagccagc
     9721 ctgatcaaga aggatgactt cgtctatctc gacgccggtt cgacgacgga acagatggcc
     9781 gatttcctgg ccgaatctga agcgaccttc gtgaccaaca gcatcccgct ggcccagaaa
     9841 ttggggcgca gcggcagccg cgtctacatc ctgcccggac gggtcaaagg cgatacggaa
     9901 gccattgtcg gcagtgatat gcgggatatg atcggccgct atcatttcac caagggcttc
     9961 ttcggagcca atggaatcgc tccccattcc ggatgtacga cgccggatga tgaagaagct
    10021 gtctgtaagc gcgctgccgt aggccagtgc ctagaccgct tcgtcctggc cgattccagt
    10081 aaattcggtt tatcttcgca cattacattt gcagacttat caatggttac gctgattacg
    10141 gccaaagatg aagaacacgt cgattaccgg ccttatcagc agattacgga ggtgcatatc
    10201 ctgtgattta tacagttacg ttcaatccat cgttagacta tgtcgtccac atgaagacct
    10261 tcacggccgg tgacatcaac cgggccgaac aggaaataat ctatcctggc ggcaaaggca
    10321 tcaacgtgtc cctcgtcctg ggaaaccttc atattccttc taagatgctc ggtttcgtcg
    10381 ctggtttcac aggccgggaa attgaacgcc tggccaaaat caacggcggc gatacggagt
    10441 tcatcatcct cgacgaaggc tgctcgcgta tcaacctcaa agtcagcgcc gatgatgaaa
    10501 cggctgtcaa tggcatggga ccgcatattc ccgaagaaaa acggcaagcc ctgctggctc
    10561 agatcgatac cctcaaagcc ggtgacaccc tggtactggc cggcagcatc cccagcgata
    10621 ttcccgatga tatttacgaa cagatcctca aacgcctcca ggaccgcgat atccgcgtcg
    10681 tcgtcgatgc cacaggcgac cttttgaaga atgtcatcaa atatagaccg ttcctcatca
    10741 aaccgaataa ctttgaatta ggcgaattgt tcagcgtcga cctccatacg gatgaagaaa
    10801 tcatcgtctg cgccaaacag ctccaggcca tgggggcccg caacgtcctc gtatctctcg
    10861 gcggtgacgg cgccctgctc ctcggcgaag acggccaggt ctaccgccgc cagtccccga
    10921 aaggcacgct ggtcaactcc gtcggtgccg gcgacagcat ggtcgctggg ttcctggccg
    10981 gtttcgaagc gtccaagggc gacctccagg aagccttgaa aatgggcatc agcgccggca
    11041 gtgcatcggc cttccatgaa tggctggcta cggaagacga tatcaaggaa gtatacgcta
    11101 ccttataaag aaacacgcat ttttgtgtag agagttgaga agagaaggag agaaataatg
    11161 cgtattgtag atttactcaa gaaacagagc atcgacctca atgcgtctgt agccgacaag
    11221 gccgctgcca tcgaccacct ggtcgacctc atggccgccg gcggcaacct gaacgacaaa
    11281 gagctataca agcagcgcgt cctcgcccgt gaacaagaag gcagtacggg tatcggcgaa
    11341 ggcatcgcca ttccccatgc caagacggaa gccgtcaacg aaccgggcct ggcttcgatg
    11401 attgtccgcg acggcgtcga ctacgaaagc ctcgacgacg aaccggctca cttgttcttc
    11461 ctcatcgccg ctccggctgg cggtgccaac gtccatctgg aagtcttgag ccgtctgagc
    11521 cgtatgctca tggacgacga tttccgcgac aacctgatga aggcccagac gccggaagaa
    11581 tacctggcta tcatcgacaa ggccgaagcc gaacaagtcg cagctgaaga agcagaagct
    11641 gccgctgaag ccgctgccga agaagcaccg gctgaaacgg aaacggccgc tccgtcggat
    11701 cgtccctacg tcatcggcgt cacagcctgc ccgacgggca ttgcccacac ttacatggca
    11761 gcagaagccc tggaaaacaa agccgctgcc atgggcgtcg acatcaaaat cgaaacgaat
    11821 ggttccggcg gtgtcaagaa ccacctgacc caggccgata tcgaccgcgc tgtcggcgtc
    11881 atcgtcgcct gcgataaaga cgtgccggtc cagcgttttg ccggcaaacc ggtcatcttc
    11941 acgaaagtcg ccaacggcat caagatcccg gaagaactca tccagaccgt cctcgacggc
    12001 aaagcaccca tttatcaggg caatgaatcg tcgtcggaag ccgtcgatgt cgaaagcgct
    12061 gaaaaagaaa gcgtcggccg tcagatttat aagcacttga tgaacggcgt atcccatatg
    12121 cttcccttcg tcattggcgg tgggatcctc atcgccctgg ctttcctctg cgacatggat
    12181 aacgccggta cggccaactt cggcagcggc acgccgctgg ctgccttcct gaaaaatgtc
    12241 ggcggcctgt ccttcagcat gatgatgcct atcctggcag gctatatcgc catgagtatc
    12301 ggtgaaaggc cggctctcat gcccggtatc gtcggcggtt tcctggcaac gacaggcggt
    12361 tctggtttct tcggcgccct ctttgccggc ttcatcgcag gctatctcat cattttcctg
    12421 aaaaaagccc tggcccatct gccggaagcg ctggacggta cgaagtccat cctcttctat
    12481 ccggtcctcg gcctggtcct catcggcgtc atcatgactt atatcatcaa tccgccgacg
    12541 tctgttttca atacgtggct cttcgacgtc ctcaacagca tgaccacatc gagccgtatc
    12601 gtcctcggct tcatcctcgg cggcatgatg tccatcgact tcggcggccc gatcaacaaa
    12661 gccgcttacg tcttcggtac ggcctcgctc atgagtgcca gcggtcaggc tgtcagctcg
    12721 ggcatcatgg cttcggtcat ggtcggcggc atggttccgc ctctggctat cgccctggct
    12781 atgctcatct ttaaaggtaa attcacaccg aaagaacgac agtcgacgat taccaacttc
    12841 atcatgggcc tgtccttcat caccgaaggg gccattcctt tcgccgcatc ggatccggtc
    12901 cacgttatcc cgccctgcgc tatcggcgcc gctgtcgccg gtgccttgtc gatgctcttc
    12961 aactgcgccc ttccggctcc tcacggtggt atcttcgtct tcggtgtcat caccaactgg
    13021 cctatgtaca tcgtcgcctt agtggccggt gtcatcgtcg cagctatcct gctcggtatt
    13081 ttccgcaaaa aacgtccgca agactgattg cgttatcgct ataaatcgta tacactatat
    13141 tatgtagctt tatctcatct tctatatttc ctaaaaggag gaatttgtca tgaaagaatt
    13201 tgaatttgtt gtcactgatc cccagggtat ccacgctcgt ccggcaggcc ttttggtcaa
    13261 agaagcaaag aaatttgaat ccaatatcag cgtattcaaa ggcgcccgca aaggcaattt
    13321 gaagaaaatc ttcaccgtca tggctctcgg cgtaaaacag ggcgaaacga ttaaagtaca
    13381 agtagaaggc gcagacgaag aacaggcagc tagtactgta gaagacttct tgaaagaaaa
    13441 cttctaattt ctttgagggg gaaagtacat gctgacgtta aaaggtaaaa gtgtcttctc
    13501 cggtatcagc atcggaccgc tggcactgtt ccatcgtaat acgatttcta cggccgtacg
    13561 gccaatcgaa gacgtcgatg ccgaaatcgc gcgtttccag aaagcccgcg aagaagctat
    13621 cggccagctc aaggacttgt atgtcaaggc tgtagacaaa gtcggtgaag aacaagccgc
    13681 tgtctttgaa gtccatcaga tgatgcttga tgacgatgac tatatcgaag gtattgaaac
    13741 caagatctgc gatgaaaaaa tgaatgctga agccgctgtc gaccagacgg ctcagaaatt
    13801 tgctgaaatg ttccgttcca tggaagatgc ttatatgcag gcccgcgcag ccgacgtcct
    13861 cgacatctcc cgccgtgtag aacagcagct ttgcggcggc ccgggaatcg acttcagcaa
    13921 ttatgaccac gtcatcatcg cagccgatga cctggctccc agcgaaacgt tacagctcga
    13981 tacggataag attctcggct ttgttacgtc agcaggcagc acgaactcgc atacggccat
    14041 cctggcccgc accctgggca tcgctgccgt cgtcagcacg ggcacacagc tccacaacga
    14101 cgtcgacaac atgacggcca tcatcgatgg cacgacgggc acggtctaca tcaatccgga
    14161 tgaagaaacc atggcggcca tgaagcagaa acagaaggaa gaagaacttc gcgtagccgc
    14221 tctggaacag gtacgcggcg tcccgacggg taccaaagat ggcaagaaaa tcaacctcta
    14281 tgccaatatc ggcaaccctg gcaatctgcc gcaggttctg gccaacgatg ccgaaggcat
    14341 cggcctcttc cgcagcgaat tcctgtatct cgaaagcagc gattatccga cggaagacca
    14401 gcagttcgaa gcctataaac aggcggctga agccctgcag ggcaagaccg tcgtcatccg
    14461 taccctggat atcggcgccg acaagaaagt cgactacttc cagctcgacc cggaagaaaa
    14521 cccggccatg ggcatgcgtg ctatccgcat ctgcctgacc tgcgtcgacc tcttcaagac
    14581 gcagctccgc gctctcctgc gcgcttcggc ttatggtaag atcgccatca tgttcccgat
    14641 gatcatttcc gtctgggaag tacagcggtg caaagaaatc cttaacgaag tccgtcagga
    14701 attggatgaa aaaggcattg cctacgataa agatatggaa atcggcatca tggtcgaaac
    14761 accggctgct gccgtcatga gtgaagaact ggctaaggaa gtcgacttct tcagcatcgg
    14821 ttccaacgac ttgacccagt ataccctggc catcgaccgt cagcagaccc agctcgatgc
    14881 cttcttcgat ccccatcatc cggctgtcct caaactcatc cagatgacca tcgaaaatgg
    14941 ccacaaagcc ggcatctggg tcggcatctg cggcgaactc ggtgcagacc tgacactgac
    15001 cgaaacgttc ctgcgcatgg gcgtagacga actctccgtc agcccaccgt cggtcctgcc
    15061 gctccgtgaa aaagtacgct ctcttgattt gagcaagtaa taaaaaaggt cttgtcgcga
    15121 tgcgacagac ctttttagct gttagccgtt agccacctgc ctcccctgtg aaggggagct
    15181 ttttggcatt tgtaggggtc gccacgtggg cgacccgtta ggaatttgga tgataatctc
    15241 agcaggctgg ccgggaaatc gtacaccccc ccaagcgggc tcgccacgtg cgagccccta
    15301 cgacctgata accattactt cagcacttct tccgagctgg tcgggaggtt aggcatatcg
    15361 gtgttgaacc attttttgta gattttctgg aattcgcccg attctttcag tttggccatg
    15421 gcgtcgttga cttgtttcaa caattccttg ttgtccttgt tgatggcgaa ggccaggtat
    15481 tccggtttgg tatcggggat attgatgaca tcgaatttat ctttgccgtc ctgggtcaga
    15541 aagtaatcgg caaccggttt gtcgagaatg gctgccggcg aaccgccgac ctggagttcc
    15601 atgatggctt cggagttatt gtcgaactgt ttgatcgtat agccctgttc ttcggcatat
    15661 ttagcgccag tcgtaccgat ctggacggca atctgtttgc cctgcaggtc ggctacggaa
    15721 tggacgccgc tgtttttcgg tgtcaagatg accagtttcg attcgtagaa cggagcggca
    15781 aaggcgactt tctgggcccg ttccttggtg acggtcatgc ccgtagcggc gatatccatg
    15841 tcaccctgct ggatgatggg gatgatacca gaaaaagcga tgttgttgaa ttcgacatca
    15901 cggcccattt ctttggctac ggcgcggatc aagtccattt caaagccctt atattccgtc
    15961 gattggtcgt tatccttgaa ttcaaaagga ataaacgtcg cattggtagc gacgcgaagc
    16021 ggtttcttcg tatcgttggc agcatctttc ttgtcactgc cgcagccggc cagcatcgaa
    16081 gcggctaata cgcctgtaag caaagcagca cctaattttt tcgtatccat acaatcccct
    16141 ccagaaattg aataaaaata aatttatgct tattattata ccatcttgtg ggaaaaagta
    16201 aagggcttgt cgcaagcgac aagcccttta tcgttgttcg tgattcatga ttcgtagggg
    16261 agaccacgtg gggcgcccgc tcctagggat gtacgaatct tccggctacg gctaatctgc
    16321 tggagaaatc ggatatatcc cgaagcgggt cgccttgaca ggacggcccc tacgagcagc
    16381 aagcagctga ctgcttgctg ctactccttc aactccggca tcttgatagg tgtcttatcg
    16441 atgaggcgga cgacgaggag ggtgatgatg cagatgatcc attcaaaata aatgacgtgg
    16501 ggcaggacgc ggatctgcgg gcagagctgc caggcaaaga ggccgatgat gccaaccagg
    16561 gtcgtccaga aggccgtgtt cttgcggcac agggacgggc agaacatggt gcaaaggaag
    16621 accagcgtga aggccgtcgt caggctcaaa ccgatgagca tgactttgac gatgtcgacg
    16681 gcgttgaagg ccagccacaa cgtaccggcg ccgatgataa ggatgattag gcggttggcc
    16741 ttgacgtagc ttgcttcgga aatatccggt ttgataaaac gtttaaaaat gtcctgagag
    16801 atgagggtac cggtaccgag gagaatcgtg caggccgtcg atacatcggc cgcccagagg
    16861 gcagccaggg tcatccctga cgagaacggg tcgagactca tgattatctg cggcagagcc
    16921 atggtcggat tgatgcccgg gaactgggct ttggcagcca ggccgaggat agccgacaag
    16981 aagccgaccg ggaagatgag caggccgccg aggatgaagc ccctgcgggc tgccttggcg
    17041 ttcttggcgc cgcaggcaat ctggaccggc ccctgagccg tgatggcctg agtcgtcatg
    17101 acgatgatcc agcccaggag tacggcaaag gacaggccgc ccatgggcga gaaccagtta
    17161 aaatccgctg ccggcagggc tgcattgatg ccgctgatgc cgccgtcgcg gagaaggatg
    17221 cggacgaggg aatagaggat gccgaggtaa atgacggtga cgctgaggat attcgataaa
    17281 ccggaagacc agaggccgcc gatggccgtg atgccgacga agacgacggc actggtgatc
    17341 atgccggcgt gcatggagaa gatatccggc atgagggcgt gtaaaatcga accgccggcg
    17401 acgtactgca gggacgtgat gcagatcatg atcagggaca ggccgatgac gctgatgatg
    17461 cgggcttttt tatcgtaact ccgttcaaag agttccggaa tggtcgtgca gttcaggtaa
    17521 cggtacttac cggcagcgac aaaacccatg atgatggcgc cgatagccca ggccgcattg
    17581 taccagccgg cagccaggcc gattttcgtc gcgctttcag cgacgccgac ggtcgaagcc
    17641 gcgccgacgg ccatacccgt gatactgacg gcgaccaggc ccgtagaaaa ctggcgccct
    17701 gcaaataaat actctgtcgg gttctgactg gcccggcgtt tgacatagaa agaaatggca
    17761 aataataaaa tgatgtataa gataacaata cacaattgaa tcgacatttt ctctccatcc
    17821 tcctttgact tataaattta aaaatttgca accttagttc cgtctatggg aacataatat
    17881 gcc
//
LOCUS       DAWABZ010000025        17145 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_906_length_17145_cov_3.174839, whole genome shotgun sequence.
ACCESSION   DAWABZ010000025 DAWABZ010000000
VERSION     DAWABZ010000025.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 17145)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17145)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17145
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_906_length_17145_cov_3.174839"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6722..6731
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9485..9494
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13650..13683
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3798 a         4843 c         4575 g         3875 t
ORIGIN      
        1 atcaataact tttattttat tatattacat tttttcagga gcagaaaccc ctaaaatagc
       61 caaactgttg gcgatagtga tgcggactgc cgtgaccagg gccagacggg ctctttccag
      121 attcttgtct tcctggatga tgcggcagtg gttgtagaac gtatggaaca taccggccag
      181 ttcgtggacg aagtgggcaa tgcggtgcgg tgcccgttcg cgggcggcac gctggacttc
      241 ttccgggaag gcggccatct tcttgatgag ttccagttcg tattcgttgg tcagcgtatc
      301 ccatttgacg tcggaccagt ccgtgctgag gtcggcgccg gcttccttgg cctgacggta
      361 aatgctgtag atacgggcat gagcgtactg gatataatag accgggtttt cgttgctgtg
      421 cgattttgcc aggtccaggt cgaaatcgag ctgcgtatcc agggaacgca tgatgaagaa
      481 gtaacgggct gcatcggtac caacttcttc gatgagttcg ttcagcgtaa tggcctggcc
      541 cgtgcgcttg ctcatcttga ccggtttgcc gtcgcggaag aggctgacca tctgcaagag
      601 gagcacttcc agctgattga cgtcatagcc gagggccgcc atggccgctt tgacacgggc
      661 tacatagccg tggtggtcgg cgccccagat attgatcatc ttcttgaagc cccggtcgta
      721 tttattctta tgataagcga tatcggcagc cagataagtc ggcacgccgt tgtcgcggat
      781 gacgacgcgg tctttgtcgt cgccgtaatc cgtcgatttc agccacaggg cgccgtcctt
      841 ttcataaatc ttaccccgtt cttccaggac cttgcaggct gcctggatgg catcgggatg
      901 gagggtccgt tcgctgaacc agttgtcata atggacgttg aaatgggcca ggtcgcgttt
      961 gagggccttg agcttttcgg cataggcccg gtccttgaag agttccagcc gttcttcttc
     1021 aggcatgtcc aggtatttgt caccgtcctg gtcgatgatg gcctgggccg tttcgatgat
     1081 gtcgtggccg tgatagccgt tttccgggat ttctgccggc ttgcccaaca gttccaggta
     1141 gcgggcattg acggaagcgg ccaggttgtt catctggttg ccggcgtcgt tgatgtagta
     1201 ttcggcctgg acgttatagc ccgcagcccg caggagattg accagggcac tgccataagc
     1261 ggcgccgcgg ccgtgaccga cgtggagcgg ccctgtcggg ttggcactga cgtattcgac
     1321 ctggatcgtg tcttctttgc gcagcggtgc atggccatag gcatcgccgg cagccaggat
     1381 ctgtttgagc gtatcgtaaa tgacgtcatt cttcaagaag aagttgatga agccggcacc
     1441 ggcgatttcc gcatggtcga gccagggata atccatgccg tcgacgatgg cctgggcaat
     1501 gagtctcggt gctttatggg cgacgcgggc cgactgcatg gcaatgttgc tggcgaagtc
     1561 gccgaattcc ttctgcggcg gtacttccag tgcgacgtcg ggatagtcgc cggctggaag
     1621 agtgccggct gcaatagcct tgttcagggc ttccgtaatg ccctgcttca ataaatcctt
     1681 catttccatt acgcttgatc ctcctgcacg gttatggtta attgtgtata attggcactg
     1741 acgccttcga tatggacgtc gtagcccaac tgaatcgtac cgactccatc gagcaagtcg
     1801 gcgcggcatt cataggtctt catctgcacg tcgaacgtgc cgaccggtgt gtcatactgg
     1861 gagcgcgtca cttttccctt gcggaattcc tgacgctggc ggagcttccc aaggcgcaac
     1921 aataagacgg accctccggg taacaccttg atgaccgtcg taacgccctt catttccgtc
     1981 agctcgcttt cttcgtagac gatgtactgc gtcccgtcct tgacgtaaaa cttgccttcg
     2041 gaaacgagct ccattttcag ctcttctccc tctttgttac gttggatact ttttacactt
     2101 actaagacag gtttcaatgt attccacctt tcctatcaca aatccaatgt tactattata
     2161 taacaagtgc tgggaaaaag aaatactttt tttcacttca tttcaatcag aaaagaccaa
     2221 tacttcttgg gcatataact gcggcgcagg gcctcgttgc ggcacgatgg cctggcccag
     2281 gtcgaagata tctgcccgga cagccggctg ggcctggtat tttctgagca gcgcttcgtc
     2341 ccggtcgatg acggccgaca aatagccgaa aaaagttcca tcataactgt aaatcatcgc
     2401 taccgctcct aaaacagact catcggttca taagccgtct gggctgacgg ccgggccagg
     2461 agcagctggc ggatgcggac cgggtccgtc gtcgctccat aatattttcc cttgcaggta
     2521 atgaaatact gggccctctt gaggacgatg cccaggcggc gcagtgtgtc atacgacaag
     2581 aaagcctggc tccgggccgc acagatgcgc cgggccgagg tgacgccgat gccggggacg
     2641 cgcagcaaag cgtggtacga cgcccggttg acttcgaccg ggaaaaactc cggatggcgc
     2701 agggcccagc aggccttggg gtccaggtcg aggtcgaaat caggcgcctc gtcagtcaat
     2761 atctcatcgg ctttgaactg gtagaagcgc agcagccagt cagcctggta gaggcggtgc
     2821 tcccgcagga gcggcggctt ggcgatggcc ggcaggttgg gccggtcacg gccgggacat
     2881 aagccgaata atagacgcgc ttcaattcca ccttatcgta taaggcctgg ctcaggcgca
     2941 ggatcgtccg gtcgctgtcc ggcgtcgcac cgacgatgag ctgcgtcgtc tggccggccg
     3001 ggacgaagga aggcgccttc ttatgatgtt tcttctcttc ccgccgctcc aggatgccct
     3061 ggcgaatgtg ccccatggga ccgaggatat tttccttcgt cttctgcggc gccagcaggc
     3121 gcagactcgg cgccgacggc agttcgatgt tgacgctcag gcggtcgacg tagcggccca
     3181 gttcacggat gagctgggca ctcgtcccgg ggatgccctt catgtggatg tagccgttga
     3241 atttcttttc gtcccgcagg atgcgggccg tccggatgag gagctccgtc gtatagtccg
     3301 gcgaacgata gacgccggaa ctgaggaaca ggccttcgat gtaattgcgc cggtaaaagg
     3361 cgatggtcac gtcggcgatc tcttccggcg tcaggatggc ccgttccgct tcccggctgg
     3421 ccgcgttgac acagtaggca cagtcgtaga tacagcggtt ggtcatgagg atcttgagga
     3481 gcgacacaca gcggccgtca gccgaccagg tatggcagat gccgcagaca gccccattgc
     3541 cgatgccccc ggcctggttg gcccgccggc tgccgctgga cgaacaggac acgtcgtact
     3601 tggccgcgtc gctgagaata tgcagtttct gttgaatccg gtcccaatcc atgataaagc
     3661 ccccctcttt tcctttatca tagcataagg ccggccctat tgcaaacgta tgttttgatt
     3721 ggccggtcgc cagtcgcagg tcgcagggca catctgtctt tcgtaggggc tcgcacgtgg
     3781 cgagcccgtc cggaatatga aaattttctc gccactaggc aaaagcgggc gccccacgtg
     3841 ggcgccccta catttgcggt ctgcggactg cgaacggcgg gcgcccctac agcggaaaaa
     3901 ggacttgccc atagggcaag tccttttcct tacatatgta atccttcgcg taatttttca
     3961 aagattttct gttcgtcttc ggtcaggtcg cggcccaggt cgatgtggag ttcttcccca
     4021 ttgctgcggg ccttgtcctg ttcgtgctta tcgccgctct tggcgctgtc gtcacggatg
     4081 atcacgctta caccacctta gatcaaagtt ttcggatcgg gatagtcttt aatgctttca
     4141 gccagtttat cggccagttc gtcgagttca cattcctggt cgtcgcggag agacgatttc
     4201 atatccatcg tcggttccag gagttcgcag catttgaact gattttcgac gtagtcgacc
     4261 atggtcttat aggcagccga tgcccagctg tggcaggcga tgacgccgat cgtgcgtttc
     4321 tggaacatga gggtcttcaa gtcgtggagg aagttttcca tgggcaggaa gaggttcagg
     4381 ttgtacgtcg gagctgccag gacgaggtgg ctgtattccc aggctttagc cgtgatgtac
     4441 gacgaatgga tcttggcgac gtcgtagagg cggatatcct ggatgccgcg ctggctcaat
     4501 ttatgggcca ggacgcaggc agcccgttcg gtgttgccat agatggacga gaaagcgatg
     4561 acgacgcctt tcttttccgg tacgtattta gcccagtgga ggtatttgtc gatgatatac
     4621 ttaatcgtag ccggcgtgcg gaatacgaag ccgtggagcg ggcagatgaa gtcgatagga
     4681 aggcccgatg ccttcttgag gacgttgacg acctgcgggc cgtatttgcc gacgatgttg
     4741 acatagtaac ggcgggccga atcgaggtag ttttcttcaa agtcgacctg gtctgcatag
     4801 acgttgccga ccgtaccgaa ctggccaaag gcatcggcac tgaagaggac gtgatccgtc
     4861 aagtcatagg acatgatgac ttccggccaa tggaccatcg gcgctttgac gaactgcagg
     4921 gtatgtttac ccagagccag ctggtcccct tccttgacgg cgacatagcg gtccgtcatg
     4981 ggatgacgga agaactgttc aaagagcttg aaggtctgcg gctggccgat aatcttggcg
     5041 tccggatatt tttccaggag agccaaagcc gagctggcat ggtccggttc catatgctgg
     5101 aggacgaagt agtccagttt acggcctttg agcaggtatt cgacgtcttc caggaattcg
     5161 tcacggcacg atttatccat ggcatcgacg acacaagtct tttcgtcatc gatgaaatag
     5221 gaattgtacg taacgccttc gggaatcggg aaaatccgtt caaacaattc cgtacggaaa
     5281 tcgttgcagc ccatccaata aataccattt ttaatttctt ctacacaatg catactgata
     5341 tgacctcctt aaaaagtaca agcagcattc atgctgtaat gtcaattctc atacccatat
     5401 cataccatct tttggacaaa caggaaatac taacaagaaa aaaagttaat taaaaatctt
     5461 tattgtttat ttgttttacc cgtatcgcga ttgttatcgg acgactggcg gctcttcgac
     5521 gaatcggaac tcttggtcgc attcgtcgac ttatcgtcag accggctgcg ggtcgtgctc
     5581 tgggactcgg aagccgattc actgcgccgc gtttcctgag aggagctgcg gctcggtgcc
     5641 gatgcgccga tggaatccgc ttcggctacg acattgtcga tgagctgttc caggacatcg
     5701 cggtcggcct gggacaggtt ggtgctttca ctgagcatgt ccttggcctg ctgggccaga
     5761 ctctgggcct gggaacgagc cgagctgcgg ctgatggagc cgttgcggta ctggaccttc
     5821 aaattgcgca gttcattgga aatgtcattg gccttgctgc ggatgcgggc cgctgccatt
     5881 tcgccgctac gctgcttacg ggcatcggca tcttgcaggc gcttgtcttc ttcccggttt
     5941 tccagcagtt cttcctgctg ctgttcggcc actttatcca gcgacgctgc cgtcacatgg
     6001 gtcccgacgt aatattcata gcccatgtag cccaggatga tgatagggat acagaggagc
     6061 atcatttcca gtttgcgcat atgcgctgcc cgtttccagc tgatgacgtt cttgcggcgg
     6121 tgttccatga tgaggacgat gatcaggacc aggacaacca ggaacagggc gatatagatg
     6181 aacaatttat gccagaccgt ggcctggagc ttgatttccg tcggattgga attggacagg
     6241 ttccacaaat aggtactgcc ctggtcatcg gctttatcgc tgttgctgac gacgcgggtc
     6301 cccgacggga aggagacgat gtattccagg tcgacgccat tttccggcat cggcgcgcct
     6361 aagcgcaggg tctggatact attttcatca aaattataat caatgtaata agtcgaacag
     6421 atgaggccat cgtgggtctt gtggacaatg tggtcgacag agctcttgct ggctccatcc
     6481 ttggtaatcg ttttcatagc ccggaagcct tcgccattat cattgttata atccataatg
     6541 gtataattgc gcttctggaa ttcatcggca tcggctttca aatctttctg ccccttagcc
     6601 gaatcggaga ctttcaggac atcctgtgtt tcgctgccat cgtcgtggat gatggtctgc
     6661 gcccggaacc ccgataagag gaagaacgct gccaacagca tgactgtcat agcaatcttg
     6721 gnnnnnnnnn ncaatcttgg caattctcgc gtacgctttc ataaaattct cgctttcttt
     6781 ctgtgccatc attcttcttt cggcggtacc atgccggtat cgacgaggtg aggccggtca
     6841 aataaggtct gggcctcttt ttcctggcgg gccttggcca tggcggcgcc attgctgata
     6901 ttttccaacg ggattccatt gacgaagatg ccttcgatga cgccttcgcc ccgcttcatg
     6961 cggatgctgg cgatgacgct gtcatcgggc tggaggttgt agatgccatc ggtcagctcc
     7021 ggtgccatgc gatagcggtc aaagccaacc tggaactgga caaagccatc ctgataggac
     7081 acgacagtgg cctgcatata gtcgccgccg acgaaaggct tcgacgccga agccccctgg
     7141 atttccatga ggcccgacgt atcccgtgaa atatagacgt aaatctgctg tcccttttca
     7201 ggcggcgtat cgtccttcca ctgcgtcgtg tcttcgggaa aaacgacgcg gacgtagtcc
     7261 gtcccaaagg cggatacttc cagccgcggg acgcagcgcc actgataggg gatgccatcg
     7321 acggcgatgt tatgccagcg ccagcccata taggcgacga cggcgaactg gacgagaacg
     7381 acgaagacga aaaagatcca gcgataacgg ccatccagtg aaaaggaaaa gtggaatccc
     7441 tgcaaaggca atggaaattt catttcatat cgcctccttg gtgagaccac ggcgagtggg
     7501 taaagtattt tttccgtttc ggtgccgact tcatcgagct ccacggcgat tccgtaaccc
     7561 gcggcttttc ttccgcttcc ggttcctgag gaacagccat ttgctggggc acggcttccg
     7621 tcttttcatg gactgtcggt gtgaccggcg acgccggcgt ttccggcgtt tctttcttta
     7681 cgtcatcagc gggtgccgtg aaagtaaatg ccggcggtgc ttcttccggc tcggccggga
     7741 cgggcggcgc tttgggcaaa tcgtcggacg tcttttcccc tgccggttcg gcaggcagtc
     7801 ccaattcagc cggcgacggg atgcgtacct ctttcatcca ctcggccagg gtgtcgacat
     7861 cgctggcggc atcctgagcc tgccaggacg gtgtctcttt aggcggaagt tcgtcctgtt
     7921 tttcttcgcc ttcacggcgg acgcgcgtcg acgaatggcg cagacctgcc cgccgggcag
     7981 cgcgcaaggc ggcagccgcc ttatccttgc gggtccgctt gaactggaag atgatcaggg
     8041 ccaggacgac gatggccagg agcggtgcca gcggggagaa ctgcgtcccg aaaataaagg
     8101 ccagcaccag gctgatgaag atgacgacgc caaatttcat atggaggctg tgtcccggct
     8161 tgaagccctg agacgtttca aagaaaccta atacgaccat gtacagcgac gaaaggacca
     8221 ggggaatcga cgaatagagc ccggcccgga cgaggatcgt ttcaaaagcc aggataaatg
     8281 ggaccgccag gtacaggacc ggtccccaca agcgattctt tacggtctgg gccatgaggg
     8341 caatcatgac ggcaaagagg acgagcgtca ccaaggccgt gacccagtga aaggcatgga
     8401 cccgggcaat gccataccaa gccgcaggca ggcaggaaat gagcagcgag ccggcagcgg
     8461 caaagcggcc gaaccagcgg aagggtacgc cccaggactg atggatgtcg atggaataac
     8521 cgacgagcat gatggtgacg gccaaactcc ccagcatgag gaaagggata tagtccgtcg
     8581 acctggctgc caggccaaaa gcagcaaaga cggtaatcgt catgacccag gaaaaaatag
     8641 acaggcgccg ttcatcacgc atggtgatga gggtatcata aaagggcacg gccaacagga
     8701 ggaaaatcca caacagcgcc gtcccgccca gcgtattcag tggcccgccg gcagccgtcc
     8761 agtacaagag agccaggtca tagacgacga gggccgcaat ggaccgcagc agatagacga
     8821 cgggcaggca gaggatggcg cagacggcga cgtaggccgt cacatcccag ccgatataga
     8881 aagactgctc ggccattgcc aggacggcaa agacacccag gcaatggact agggccacgc
     8941 cttcggcgac gaggctcccc tgccgttcct ggaacatggc ggcgccgaca ccgatctgag
     9001 taagcaggag caggccacag gccaggccca tgcggaccgt aagtgataca tagtaccaga
     9061 actgggaaag gccccaaatc agggcgatac cggcagcggc caggccgatg acggccagca
     9121 gggcatataa aggcagggtc gtcaaaagag ggttcctttg ttcgtccctg ccgtaagatt
     9181 ttcgtatttt ttgctgggat tcttcactga tccagccctt agaaacccag tcagcgacct
     9241 tgcggtcgag ccattcgttg gtatttcgtt tcatacttgc ctcccaggca cctatcccgg
     9301 aacgaggcag tccgggaccc tatactcatg aataggtttt gtgtaatcag gcaaaaaaag
     9361 gcatacctgt gtactcagta taccacaaaa agaacgattc ccctatggcg taacgtaaaa
     9421 ttttcagaaa aaaggcgcta tcctatgaag acagcgcctt ttttctgtag gggcgccacg
     9481 tgggnnnnnn nnnnatgaat catccaaatt cctaacgggt cgcccacgtg gcgaccccta
     9541 caaacttcat actacttttt agtggttagt ggttagcggc tagtggttag gggtcagtaa
     9601 cgcaggtcgt ctctccgcga accacgagcc acgaactacg aaccactaat tacttattta
     9661 acgatcaata cagggcagac ggaatgggtg acgacgtagc tgctgacaga acccatgaac
     9721 aagcctttca acggcccgag gccgcggctg cccatgacga tgaggtcagc attgtacttc
     9781 ttggctaccg acaagacggc cggtcccgga gaaccgactt caaagacact cttgacttcg
     9841 atgttctggg gaatggattt ggcgaattcg tcgaggatac ctttcccttc ttcttccatg
     9901 tccagtgcga cctgttccgt tacatagccg ctggcattgg ggatctggtc gaagttagaa
     9961 atgaccgata cgatattggc gacatagacc agtgtaatcg tagcccctaa cgacgaagcg
    10021 atgacgacgg catgttccag cgcacgtttc gagttaaccg aaccatccgt cggcacgatg
    10081 atgttattat attttttcat atgaatccct ccttattaag gcctgttctt atccttaaca
    10141 tatatattcg ccatgacttt tacggattcc ttctttgtca tccaattttt ttcagaaaat
    10201 gcctgctttt tatttttata aaattattta gcttctactt aaatgactat gagaaatctg
    10261 aaaaatgagt ataaactttc atgtctctat gctataatac tgaatatgat tgtaaaattt
    10321 ttaagtttct ctgcaatcag ataataagaa aggtggtttt tatgacacat gaactcgtaa
    10381 gtgcgacctt gcccgtatgg agtatcatcc cattcgccgg gatgctctta tccatcgcca
    10441 tcgtccccct cgtccgtccg gaatggtggg aaaaacacat gctcggcgct gcgttcgcct
    10501 ggtctctttt attcctcatc cccttcgccg ctgcctatgg cgtcggtgag tcgtggttcc
    10561 gcctgctcga atcggtgctc ctcgactaca tccccttcat cgtcctcctc ttcggcctgt
    10621 tcgtcgtcgc cggcggtatc gccgtctgcg ggaccttagc cgggacgacg aaaatcaact
    10681 gtctcctgct cttcatcggc accttcctgg ccagctggat cggtacgacc ggcgcagcaa
    10741 tgctgctcat ccgtcctatc atccgggcca atgcctggcg caagaacaaa gtacacctca
    10801 tggtgttctt catcttcctc gtcgccaaca tgggcggctg tctgacaccg ctcggcgacc
    10861 cgccgctgtt catgggcttc cagcgcggcg tcccctttac ctggaccttc catctcctgc
    10921 ccatcctgat tttcaacctg gtcctcatgt tcgccttatt cgccgtcatc gacaagcatt
    10981 tttataataa ggaaataaaa gaaggccgta cgccgctcga cttgatcgat gaaaacgaca
    11041 agcagcccct ccgcctggaa ggggcccaca acctcatctt catcgccatg atcatcgtcg
    11101 gcgtcctggc caacggcctc ctgccggata tgttcccggc tttcgccaac ggcgctggcc
    11161 tgcacattta cgacgaaatc gtcttcccct atgctacact ggcagaaatc gtcatcatcc
    11221 tcgtcgcagc cttcctctcc atcaagacga cgaagacgtc gacgcgggaa ctcaacgaat
    11281 tcacgtatgc ccctatcctg gaagtcgcta agctgttcat cggcattttc atcaccatga
    11341 tcccggccct gatcctgctc aaggcccatg gcgctgaact gggtctgacc catccgtggc
    11401 agatgttctg ggcagccggt gccctgtcgt ccttcctgga caacacgccg acatacctgg
    11461 tcttcctcca gatggccggt gccctcggcg ctacccaggg catcacgacc tctgtcggga
    11521 ccgtatccca gctcatgctg gaagccatct cggccggtgc cgtcttcatg ggtgccaaca
    11581 cgtatatcgg caatgcaccg aacttcatgg tcaaatccat tgctgaagaa aacaagatca
    11641 aaatgccgag cttcttcggc tacatgggct ggtccgtcgc catcctcatc ccgttgttca
    11701 tcatcgacac cgtcgttttc ttcctgtaag ttgtggctag tggctcgtgg ttagtggcta
    11761 gcggtcccat ggaccgcatt tgtaggggcc gtcccagcgg gcggctgacg ctggctgctc
    11821 gctatttctc catcagaacg taaacaaggg actatcgcat aaaggcttat gtcattatgc
    11881 gtagtccctt ttatcttata gtttgtagtt cggagtttgc agtttgcagc agatggtttt
    11941 aaatttttaa ccatccacta caaactaaaa actacaaact tcaaacacaa aaaagctgtc
    12001 gcaaaagcga cagctttttt aactggtgat ccatccgaga ttcgaactcg ggacaccctg
    12061 attaaaagtc aggtgctcta ccagctgagc taatgaatca tggctggcag gggtagctgg
    12121 attcgaacca gcgcatagcg gagtcaaagt ccggtgcctt accacttggc tataccccta
    12181 tggggcgagt atagggattc gaaccctagc gtaacggagc cacaatccgc cgtgttaacc
    12241 acttcaccat acccgccata ttagattatc tgcgtcggcc gcgctacatt agcgccgtca
    12301 acgtagataa tcttaccacg agtcgctcac aaagtcaagc actttttcaa acttttttag
    12361 cgttcttttt caccgtacaa taaatctttg acgaaattcg gcaggacgaa ggcgctctgc
    12421 tggatgcctt cgttatagta ccgcgtcgag aaggtccgtt tacggttcgg ctgccacgta
    12481 aggggatcgt atttcttcga gcccatggtg aagcagtgca tgcctgacgg atagaggggg
    12541 atgaaggccg tgtacaggcg ggtgatgggg aagatgtccg acacgcagtc gaagatatcc
    12601 ttgaccagct tgcggtgcat gaacggcgat tcggtctgct gtacgaagag gccgtcgtct
    12661 ttgagggccc tgaaggtatc cttgtagaaa tcatacgaga acaggccttc gccgggacca
    12721 acagggtccg agcagtcgac gatgatgacg tcgtagtaat tttctgcttc ccgcatgaac
    12781 gcgatgccgt cgccgacttt gacagtcagt ttcgggtcgc cttcgatcat ggccttggcg
    12841 atttccggga aatactgctt ggataattcg atgaccttgc cgtcgatgtc gaccatggtg
    12901 accgattcga cttccggatg gcggacggct tcccgggcag cgccgccatc gccgccgccg
    12961 atgatgagga ctttcttggg attgggatgg aggaacatgg ggatgtggac gatgctttca
    13021 tggtacatga attcatcttt gatggacgtc tggaaaacgc cgtccaggac gagcatacgg
    13081 ccgaattcct tagaatcggc gacgatgata tcctggaact gtgaatggcc gcggtagagg
    13141 atatccgaca cttccgtcgt caaatccagg tagggggact gatgttccgt tacgaatttt
    13201 ctcatgacaa ggctccttta tcatataaag tatagttttc attaagacat tgacactttt
    13261 attctaccat atttaccacc atttgtcgta actcattttg ggtcgtaaaa aaatcctctg
    13321 ccccacccct tacagaataa gaggaatatg gtacaatata aagtagactc tttatatata
    13381 gtctttatca tatagaaatt tataagaaag aatcggttgg taaaccgata gggtgacaaa
    13441 acatgaaaaa tctcttacat atcggcattg acatcggctc gacgacggta aaaatcgccg
    13501 tcctcaatca tgccaaacag tgcctccacg catgctatgt ccgccactat acggatatac
    13561 gccaaaaagt ctgggattta ctgcaaaacg cctatgaaat ctttggcgac cgtcaaatca
    13621 ccatggccat caccggctct ggcggcatcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13681 nnnggcagac tacctgcaca tccccttttt gcaggaagtc atcgccggta ccaaagccgt
    13741 ccgcacgtat tatccccagt cggacgtcat catcgaactc ggcggtgaag atgcgaagat
    13801 cacctatctg accggcggca acgaacaccg catgaacggc aactgcgccg gcgggaccgg
    13861 ggccttcatc gaccagatgg cgaccctcct cgacaccgat gccggcggcc tcaatgaaat
    13921 ggccaagaaa gccgacacaa tttaccccat cgccgcccgc tgcggcgtct tcgccaagac
    13981 cgacatccag gctctgctca acgaaggggc cagcaaggaa aacgtcgcgg cgtcggtctt
    14041 ccagtccatc gtcttccaga ccatcaccgg cctggcctgc ggccgcccca tcaaggggac
    14101 agtctgcttc ctcggcggcc cgctgaccta cttgtcccag cttcgggaac agttcgccaa
    14161 gacgctccac ttgacgccgg aacaggtcat cgccccggaa aacgcccagg tctacgtggc
    14221 catcggcgcc gccctgggca gtatagacac gaaacccgtg tccttcatga acctcatggg
    14281 ccagctgaag agcgtcgacg aatcgacgct ggccaaatcg gaccgcgtcg aaccgctgtt
    14341 caacaatcag gctgagctcg acgaattccg ggcccgccac agccagcata ccgtccccca
    14401 ggcggacatc accacctata gcggcccgtg ctacctgggc atcgacgccg gctcgacgac
    14461 gattaaagcc gtcgtcctca gcgaagacat gaagatcctc tattcgcact atcagaacaa
    14521 cgaaggcagc cccttggcgg cagccaagaa aatcgtcgct gaaatctacc gccgcctgcc
    14581 ggaaaaggcc ttcatcgcca aggccggcat cacgggctac ggcgaatcgc tgctcaaaga
    14641 agcgctgcac ctcgacctgg gcgaagtcga aaccattgcc cactatcagg cagcgtccca
    14701 cttctgcccg gacgtcgact tcctcctcga tatcggcggc caggacatga aatgcagccg
    14761 catcaaagat ggtcatatcg aagatatcct gctcaacgaa gcctgctcgt cgggctgtgg
    14821 ttcctttctg gatacctttg ccaaatccct gaaaatgtcc atccaggact tctcccaggc
    14881 cgccctgctg gccaagagcc ccattgacct gggctcgcgc tgcacggtct tcatgaactc
    14941 caaagtcaag caggcccaga aagaaggggc cacgctggcc gacatttccg ccggcctttc
    15001 ctattcggtc atcaagaatg ccctttataa agtcatcaaa gtcaaagatc cggccaaatt
    15061 aggaaagcac atcgtcgtcc agggcggtac tttctacaac gacgccgtcc tgcgggcatt
    15121 tgaaaaactc ctgggccgcc acgtcatccg cccggccatt tccggcctca tgggggcctt
    15181 cggcatgggc ctcatctgcc gcacggccta ccacgaagac catgccaaat cgaccatcgt
    15241 cagcccgaaa gccctggaag aattgcaggt cacgacgtcc atccgccact gcggcttgtg
    15301 caccaacagc tgcctgctga cggtcaatac cttcaacgac ggccgttcct tcatcaccgg
    15361 caaccgctgc gaacgaggtg ccgccggctc gacagcagcc cgcaagaagc ccctgcccaa
    15421 catgtacaag gtcaaatata acctgctctt cggctacaag cccgtagaaa atgcgccgcg
    15481 cggcaccgtc ggtatcccgc gggtcctcaa tatgtacgaa gactatccct tctggttcac
    15541 gttcttcacc aaactcggct acaccgtcgt cctctcggac ccgtcgtcga aggccctgtt
    15601 tgaaaaggcc atggacacgg tcccgtcgga ctcggcctgc tacccggcca aactggtcca
    15661 cggccacatt gagaacctcc tggaaaaggg cgtcgaccgc atcttctatc cgtgcatcca
    15721 gaacggcccc agcgaaggca gcaaagacaa taccttcaac tgccccatgg tcatgagcta
    15781 tcccgaagtc atccgcaaca acatgggcga acgcctggcc gaaaccaata cgccctacct
    15841 ctgccccttc ctgcccctcc atgacaagaa acgcatcggc aaacgcctgg ccgaagaatt
    15901 gaaggcctgg aacctgccgg ctcacgaagt caaagccgct gccgctgccg cctgggatga
    15961 agaagaagca tataaacaga agatatacga cattaccaaa aaaatcctgg ataacctgga
    16021 aaagacgggc cagcgggcca tcgtcctctg cggccgtccg taccacatcg acccggaaat
    16081 caaccacggc atcccggaac tcatcaacat cctaggcctg gccgtcctca ccgaagacgg
    16141 cgtcgctccc ctgggccagt tcccgggcca cctgcgcgtc gtcgaccagt ggtcctacca
    16201 ttcgcgtatc taccgggctg cccagtacat cacacagcat ccgaacctgg aactggtcga
    16261 actcaattcc tttggctgcg gccttgacgc catcgtcgcc gaccaggtcc aggacatcct
    16321 ggcccacggc cacaagatcc atacactgct caagatcgac gaaggctcca acctgggtgc
    16381 catccgcatc cgcctgcgct cactgctctt tgtcatgaat caccgtccca aagaagatca
    16441 cgtcgtcgaa ccgtattctt acgaaaagac gattttcacc aaggaagacg ccaagcagaa
    16501 cgtcatcctg gctccgtcga tgacaccgat tcatttcccc ttgttcagcg ccgcctttga
    16561 acacgaaggc taccgcgtcg aactcctgcc ccaccagggc cagaaggcca tcgacacggg
    16621 cctggcctat atccacaacg acgcctgcta tccggccatc atgagcctgg gccagatcat
    16681 tacggccctc aaatcgggca aatacgacct ggaccacacg acagtcatga tttcccagac
    16741 cggcggctgc tgccgggcga cgaactacat tggcatgatc cgcaaggccc tgaacgacgc
    16801 caacatgtcc caggtcgcca tcttgtcgct gaacctcaag ggcatgaaca agcagcccgg
    16861 cttccagccc ggcatcggct tctggcatcg cctggtcatg ggcatgatct acggcgacgc
    16921 cctgcagcag gtcctctacc gcacgcggcc ttatgaaaag gtcaagggga cagccgaagc
    16981 cctcttccac aagtggcaga agaaatgcgc cgaacagctg aagaaggccg atttcaaatc
    17041 gttcaagaga aatatccgcg acctcatcga cgaattcgac cacatcgaca tgagtgaaac
    17101 catgaagccg cgcatcggcc tggtcggtga aatctacgtc aagtt
//
LOCUS       DAWABZ010000026        16681 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_931_length_16681_cov_3.307771, whole genome shotgun sequence.
ACCESSION   DAWABZ010000026 DAWABZ010000000
VERSION     DAWABZ010000026.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 16681)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16681)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16681
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_931_length_16681_cov_3.307771"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3191 a         4541 c         4931 g         4018 t
ORIGIN      
        1 caatagtgaa tcccccagac aaccaatccg cattcatgga aaccgatcta tggttttacc
       61 gatgaaccgc agatcgatga cgattttatc atgccctctt tctattgtga ccaacatccc
      121 gattcccggt tcaataaata catgaaaatc agcctgttta cccaggatgc cgatttgact
      181 atcgtaaaag atgaaatgaa agtgtatcga aatggcaaag tagaaaagcg tcttcccatt
      241 tctggaaaag aagaagctgc ccatctcctg tctgacattt tccacatctc agttccccat
      301 aattatcgtc ttttaccata aatttaaaga atatgaccat acagctatga tatttttgca
      361 tacagcaaaa gggcttatgc acgattctcc gtgcaataag cccttttacg ttgttagtaa
      421 ggtcaaatat caagtctcga acgtacttcc tgatccagca cctgcagcag gccgatgtag
      481 tcttctttga tgtggcggta gatgtcctgt gccgtttctt cattataggt atgaaaagaa
      541 aggttgcgtt tgacctgcat gtccagccat ttttccgcat cgccgatgag atgttgtttc
      601 caggcttcgc ggatactgct gcgtgggctg gtcgcttcga tgccttcata gtccaggacg
      661 gccttcatca gtttccaggc cagttcaaag gtaaattcaa aacgctggat ggttgcatcg
      721 agatagaggt cgtcttcgtc caaagtcttg gtcagggcaa tttccaggcg gttcagggct
      781 ttgtggtagt cttcccactt cagccgtaat tgttctttcg tcatcatcca ttctcctttg
      841 ccgtcgacag cccagagcag tttcccttcc ctgtcgatgg catctttcaa tttctgattc
      901 ggctgacgcc ggtaatcaat gagatcaaaa gtaaagggca gctggctctg gtcgaaatcg
      961 agctgcagcg gcgtcaccag attttccgta ctggcaatgg ccaaatccac atcggaaaca
     1021 ggcttataat cgccacgagc ccgcgacccg aagagtttca cccaatcgat ccggttgcca
     1081 tagcgggaca aaatagcaaa gatctgccgc cactgtctga cactcaatcc gtacatagta
     1141 ctcctccttt gggacggctc ctgcttaacc tctccggccc tgcgggccac ctcccctatg
     1201 aagggaggct tgggcgcccc tatgattggc gggcggccgg cgacctgcga cctgcgggcc
     1261 gccttttagg cggcccctac gattggcact tcgggcagta gtgggtgccg cggccgttca
     1321 gtttggtgta ttcgatggtc gtgccgcaga cggtgcaggg cgtgccttcg cggccgtaga
     1381 cgcggaggtt ttcctggttc tgcccttctt tgccgttgct gctgatgaag ttgcggattg
     1441 tcgtgccgcc gtgttccagg ccttcctgga ggacggcgac gatggcttca tgaaggcggg
     1501 ccgcttcttt ttgggtcact gtatcgctgt ggcgcatggg ccggatgccc gcccggaaca
     1561 gggcttcgtc gacgtagatg ttgcccaaac cggccacttt ggtctggtcc aggaggagca
     1621 tcttcaccgg ccggtggcag cccttgagga ggtcatagag ccgctccgtc gtgaagtccg
     1681 gactgatggc gtccggtccc aggttgtcgt aacctttgac gcctgtcgcc ccctctgacg
     1741 gcacgagcca caggcagccc agggtgcgga tatcgcggta gaggaggcgc ccttcgctga
     1801 gcgtaaaaat aagatgaatg gcccggccag catactggcc gggcttttct tcatagagca
     1861 ggctgcctgt catgcgcaga tggaccagaa aggaccaggg accgcggcag cggacaaaga
     1921 ggtacttgcc gcggcgttcc acgccttcca gggtctggcc ggtcaaggcg gctgcaaaac
     1981 ggtctgccgt cgtattcttg atgagcctgg gcaggcctat ttcaacggcc gtaatggcct
     2041 ttccccggac acgctcgtcg aggaaggccc gtatcgtttc tacttccggt aattctggca
     2101 tggtctcacc tacttcgctt cttcccagtt gacgccgctg tggatgtcga cgacgagggg
     2161 cacggataaa tcgacaatgt gttccattgt atccttgagg agctgttcca cttcgtcctt
     2221 ttccccggcc gggacttcca ggaccagttc gtcatggacc tggaccagga gctggctctt
     2281 cagatggcgt ttttccaatt cgtcctggac agcgttcatg gccagcttga tgatatcggc
     2341 ggcgctgccc tggataggcg tgttcatggc cgtccgttcg gcaaaggacc ggcgcgtgaa
     2401 gttcttgctg ttgatttccg gcaacgcccg gcggcggccg aacatggtga cggccatgcc
     2461 cgattccttg gccgaggcga tgaggccgtt catgtagtcg tggaccgtgt tgtagcgggc
     2521 aaaatacgaa tcgatgtatt ccttggcttt ttggcgggaa atggagatat tccgggccag
     2581 gccgaagtcg ctgatgccat agatgatgcc gaagttgacg gccttggcat gggaccgttc
     2641 ttccggcgtg acctcgtcca gggggatgcc gaagacttcc gacgccgtcc ggcggtggat
     2701 gtcctggccc ttcttgaagc cgtcgatcat agtcgggtcc tgggacaagt gagccaggag
     2761 gcgcaattcg atctgcgaat agtcgccaga gacgatgtag tcatagcctt cgccgggcac
     2821 gaagagggag cgtatcttgc ggccctgttc ggtccggatg gggatattct gcagattcgg
     2881 gtcggaactg ctcagacgtc ccgtcgacgt gaccatctgg ttgaaatggg tatagatgcg
     2941 ccccgtttcc ggcacgatga gcggttccag gccatcgaga taagtggaaa tgagtttctt
     3001 caaggcccgg aagtgcagaa tcttgtcgat catgggatgc ttgtcgcgca gggcgtcgag
     3061 gacggaactg tccgtcgaat agcccgtctt ggtcttcttg atgaccggca gctgcaactt
     3121 ctcgaagagg atgacgccga gctgtttcgg cgaattgatg ttgaaggtct cgccggcgtc
     3181 gtcgtaaatg gcttcttcca gggcatcagc ctggcgggac aattcgctct tcatggtcat
     3241 gaggcggtcc gtatcgacag tgaagccgtt catttccatg acggccagcg tatggatgag
     3301 cggttcttcg atggtatcgt agagctgtgt cagggatgcc ttatccaatt cagctgccgc
     3361 tttgggccag acctggaggg caaaagcggc atcgcaggcc agcttctgtc cgtcgtcttc
     3421 tgtgccttcg tccaggctga cgtcgaaggt ttcggaaata tacgtcaggc cataggacgt
     3481 ccgcgtcggg tcgaggagat aggctgccag gatgatgtcc ttcaagggca ggcggcccgg
     3541 agcgacgcgg gatacgacct tggtcaggtc cttgccgtca tcggtgacga cggctgccgc
     3601 ttcggtcagg acggcgagat aggcgtcgta atcgctttcg ggaacgacga aggcgccatc
     3661 atcgacggcc atggccaggg ccgtcacagc cgggatgggc tggctgccgt catagcgggc
     3721 ggcgacggcg acggtcttcc ctttcaggct ggccgcgccc ggtgcgtcca tgacctggac
     3781 ggccgctgcc ggtgccgcgg cgtctgccag atgagagaag cggtcgaagg cggcaaagcg
     3841 ttccagaaga ttgcggaagc ccaggctgcg gaagagctgg gccacgtctt cctggcgcgg
     3901 ctggggctgg aacatgtcca tggtgtaatc gaggggcacg tcacagcgga tagtggccag
     3961 gtccttggac aagacggcct tgtccttgtt gtcggccagt ttttcctgca gcttcttgcc
     4021 cttcacttcg tccaggtgtt cgtagacgcc gtggacgctg ccgaactggc tgatgagctt
     4081 gagcgccgtc ttttcgccga cgccggggac gccggggata ttatcggccg tatcgcccat
     4141 gagggccttc atgtcgatga cctgggccgg cgtataaccg tattcggctt ccatgacggc
     4201 cggcgtaacg gccagcatgt tggtgatgcc cttcttggtc agatagaccg tcacgtcgtc
     4261 cgtgaccagc tgcagggcgt cgcggtcacc ggtgacgaca gcgacggcat agccatcggc
     4321 tgccgtgcgg cgggccaggg tgccgatgat gtcgtcccct tcgtagcctt cctgttcgag
     4381 gacgcagatg cccaggcagg ccaggacttc gcggatcagg gcgaactggg gccgcaggtc
     4441 atccggtgtg ggcttgcgcg tagccttgta gccgtcgaac aagtcgttgc ggaaggtgtg
     4501 cttgctcttg tcgaaggcga cggcaacgta ctgcgggtcc agttctccgt acaatttcaa
     4561 taacatattc aaaaaaccat agacggcatt cgtcgggtgc ccctgggcat cggtgagatg
     4621 cgggatggca taaaatgccc gatagagcag actgctgccg tcgatgatga cgatattttc
     4681 tttcatagaa cctccctacg cccactcacc gatgcaacag ggagctgacg atgctgtaga
     4741 tacagtagat gacgaagatg cccaagagat agggcatggc catgatgata aagcccaggg
     4801 cgccgatgat gacggcgacg atgcagaaga cgatgaagcc cagggccagt ttgacacgcc
     4861 acgaaaggcc ggacgtgacg atgtggatgc catgatgcgc ttcctgttgc tgacgcagcc
     4921 gttcttcata gagggacttc tggtcttgga ccgtcccatc ttcatcgatg gtctggccgc
     4981 ggaaactatc ccgttcgtga tccgaaagga tttttgggtc ttctgaattc atagatactt
     5041 ctcctcctga ttagtagtta cggctcttca tgcggcgttc gatgtcgcgc ttggcttctt
     5101 tttcggccag ggcctgccgc ttgtcgtaga gcttcttccc tgttaccagg gcgacggtca
     5161 ctttgacctt ccccttcttg aaatagaggt tgagcgggat gagggtatag cctttttcct
     5221 ggatcttgcc caggagcttg atgatttcat atttatggag caagagcttg cgcgtccgca
     5281 ggggatcgtg gttgaagcgg ttgccctgat catagggact gatgtgcatg cccaggagga
     5341 agacttcgcc gttctcgatg cggcagaagc tgtccttcag attgaccttg ccctggcgca
     5401 gggacttgac ttccgtcccc gtcagggcaa tgccggcttc atacgtttca tggataaaat
     5461 aatcgtgacg ggccttgcgg ttgtccgaaa tatatttcac accctttgtc ggtgacgttg
     5521 ccaaaacgaa ttcctcctat ttcttcttct ttttgccgcg gcggctcttg ctgcccttgc
     5581 ccttgcacgc ggctttgcgg ccggatttct tgtccttgca gcctttctta tccttcttgt
     5641 ctttcttgcc cttcttgccg atggctccgg cgaaccagtt gtgcttggtg tcggtgtcct
     5701 tcttatcgct gtgtttccgt ttatggcgcc gttcggcact ggccgccatg agctgctgca
     5761 cgtcttccag cgattcgtag cggcccggga cgaagtcgat ttcgcatttt tccacattga
     5821 cctgagccag ggtgacttcc atggcatcgc ctaagcggta gaccttgccg ccgtgctggc
     5881 cgcgcagggt gtacgacgct tcgtcgaatt cgtagtcgtc gtcagtgagc agggaaatgt
     5941 ggaccaggcc ttcgatgccg ttttccaggc ccacgaagag gccgaaagac gtaatgcccg
     6001 taatgtgcgc ttcaaaaggc tgaccgatga agggcagcat gtactggcac ttcttgaggt
     6061 cgtctgtttc ccgttcggct tcgacggcaa tgcgttcgcg cgagctggcc tggtcggcgg
     6121 cgatggtatg gtacgacagg gactgctgcg attcttcctc ggtcagcttg ccccggcgct
     6181 ggtactggcg gatcagacgg tggaccatga ggtccgggta gcggcggata ggcgacgtga
     6241 aatgagtata acattccgaa gccaggccga agtggccggc attagtcgtc gaatagcggg
     6301 cctgctgcat agagcgcaga gcgatggtct ggacgacggc ggcttcatcg gtgcccttgg
     6361 tcatttccag gagcttctgg aagtctgccg gcttgaccga ttccgtatcg ggcatgggca
     6421 ggttgaaact cgacaggacg gtgcgcatca tatccagccg ttccggttcg ggcagttcct
     6481 ggatacggta gaccgacggg ttttcgctgt cccggagata gcaggctacg gtttcattgg
     6541 caatgagcat gctttcttcg acgatctgtt cggcaatggt ccgttcccgt ttttccagac
     6601 gcagcggtac gccgtccgga tcaaggatga ctttgtattc agggaaatcg aagtcgatgg
     6661 ccccgcggcg gaggcgcatg ttcttgagga tcgtcgacaa ggcccgcagt tcgtggagca
     6721 tgggcatgaa gggctgcaag tcgtcaggca cgatgccttc caggagggcc ttgcggattt
     6781 cgacgtagtt gcagcgccgt ttgacgcgga tgacggccgg gccgatgtcg gccttgagga
     6841 ccttgccttc ttcgtcgatt tccatgacgc aggtcatgga gtaacggtct tcattggcgt
     6901 tgaggctgca gatgccgttg gacaggaccg tcggcagcat ggggatgacc cggtcgacaa
     6961 ggtagacgct ggtgccgcgg ttatatgctt cctggtcgat ggccgatccc gggcggacgt
     7021 agtagctgac gtcggcgatg tagacgccca gtcggaaatg gccgttgggc aggcggacga
     7081 cgtcgatggc atcgtccagg tccttggcat cttcactgtc gatggtgatg agctggcggt
     7141 cgcggtaatc gcggcgccct tcttccaagg tgaccgtcgt gtcgatcttg gcggcttcct
     7201 tcttgacgtc gtccgggaag tcgaaaggca ggccgtggcg ggccatgatg accttgatat
     7261 ccaggtcctt gtcgccgtca tagccgagga cttccgtcac ttccccttcg gctttgcgcc
     7321 cttcttcggg ccacttggtg atcttgacca ggacgcgggc actgctgcgg gcgccgccgg
     7381 tcttgtcgag gggcacgtag acgtcttcgc ggatgcgttc gtcgtccggc gtgacgaagg
     7441 cgctgtgttt ttccagctgg aacgtaccga ccagggtctt gttgacccgt tcgacgatgg
     7501 atacgatttc cccttcctgt ttatgacggc catcgccgct gtgcaggatg cggaccgtga
     7561 ccttatcgtt attcatggcc gtatggcggt tctgttcggc aatgtatacg tcctccgtca
     7621 ggtcctggct gaggacgaac ccgaaggtcg gcgtataacc tttataaata ccggtgattt
     7681 cctggggctg tttggccgag atataccggt ctcctgcgat tctcatgagc ttttcactcc
     7741 tcactaattc ttcaaatgtg tcaaatacgt cagccagccg ggcgccggtg atgccgagct
     7801 gttcggcaca gtcttcgacc gttgcgccgc cgctgtcttc attacatata cttattatat
     7861 catgagccag ctgtaaatcc atagttcccc tcctcaaatc gccgttctgc aaaggataaa
     7921 aaaggagccg ccgcatatgc gacagcccct aagaattcat taaaaagtat gcatcagtct
     7981 gcctaacagc aatgtacaaa tggcaaatgc tatcgacagg acgatagtca gtttggaaag
     8041 cagggcatcc atgccgcggg cccggctccc gaacaagtct tcggcaccgc cgccgaaacc
     8101 ggcgcccatg ccctgggtct tgctcttctg tgccacgacg acaccgatga tgagcagtgc
     8161 caaaatgaca acgatgattt ctaatgctgt gatcacgtta atcctcctca cacaatgacg
     8221 ctaacagtac tattgtacac tcatttagct tttctatcaa taggttcttt ggctttctca
     8281 tcgggttccg tttctttttc ggcattgcgc gtttccagat aatgttccag cttgcgtttg
     8341 acccgctgca gggcattgtc gatggacttg acgtgccggt gcagctgacg ggacatctcg
     8401 ttgtaggatt tgccgtcgag ataggctgtc aggacctgcc attccaaatc gctgaggatc
     8461 tcattcatct tcagttcgat atcattgaat tcttcgcggc tgatgaccag ctcttcggga
     8521 tcgctgacgc ggatgccgga caggatgtcc tgcagcgtcc ggtccgattc gtcttcatag
     8581 acgggcttgt tgagagacac atacgagtta aggggtatgt gtttctgccg cgtcgccgtc
     8641 ttgatggccg tgatgatctg ccgggtaatg cataactcgg caaaggcccg gaacgacgac
     8701 agcttgtcgc cgcggaaatc ccgcgtcgcc ttgtagaggc cgatcatgcc ttcctggatg
     8761 atgtcatccc ggtcggcgcc gaccaggaag taggcgcggg ctttggattt gacgaagttg
     8821 cgatacttgt tgacgatata atcggccgct ttgccatcgt gctcttcctg ggcgatgcgt
     8881 acgatttctt catctgtcat gcctgcaaag cggtcatgtt tttcttctgc attggacgct
     8941 gcctgcataa tcgttacctc gcttatttta aattattcgc ctgcctgccg ctgccgcacg
     9001 acttcataca tgagcagggc cgctgcgacg gaggcgttga gggagttgac gtggccgaac
     9061 atgggaatct ggaccaggac gtcgcacgtc tccttgacca ggtgcgagat gccccggcct
     9121 tcactgccga tgaccaggac gaccgggtcc gtcagggagc tgtggaaata gtccgtctcg
     9181 ccgtccatat cggcgccgat gacccagaaa ccgcgcttct tgagctgctt cagggtctgg
     9241 gcgatgttgc cgatctgcac gaggggcatg tattcgacgg cgccggccga cgtcttgccg
     9301 acagtctcat tgatgagcgc cgtccgccgc ttgggcagga ggacggcgtc gacgccggca
     9361 cattccgccg tgcggatgat ggcccccatg ttgtggacgt cttcgatgcc gtcgaggagg
     9421 accaggaacg gtaccttccc cttttcatca gcagcgatga gaacgtctgt caaatcggcg
     9481 tattccacgg gcttggcaaa ggcgatgacg ccctggtggc ggttccccgg tgccaggtgg
     9541 ttgaggacct tcaggggcac gtcttcgacg aggatttcct tccggcctgc caggtcgcgg
     9601 atgcgctgca gcggctgcga ctcattgccg gcagcgacgt acagcttggt gatggaccgg
     9661 ccggccttca gggcttcggt gacgctgttg cggccgatga tgatatttgc ctcactcatt
     9721 ccattcctcc atgtttttcg ttacaatagg ctttcgtttc ttcatagacc tgggccatga
     9781 cggcgtccag gcgttccgtc cggccagcca tgaccaggta gcccaccagg gcttccaggg
     9841 ccgtgctgtc gtggtactcg gcgacggtgg cgctcttcgg cgccagggta taggcattgc
     9901 gggcccgctg gcagacggcc ttttcgtcgt ccgtcaggcc gtcgtggatg cggcggtaga
     9961 cataggcctg ggccttggcg gatacgaatt ctgccgccag cgtgtgcagg acctggacgt
    10021 gggggatgcc cgtttccacc aggcgccggc gcagggtcag ggcgtaatag gcgtcaccga
    10081 cgaaggccag gttgatcgag tcgagatggg ccgcatcctg gtcgggcacg tccaggcgct
    10141 tgccgtcggc aaaggcctgg taggcccgtt tcttcagtgc ttgcagttgt ttgaatttca
    10201 ctttttccag cgcgctccct gcggcgaatc ttcgatagtg atgccaagag cggccagttt
    10261 atcgcggatg gcgtcggcca gggcgtactg tttctgggcg cggcattcct gacggatgtc
    10321 gaggatggcc tgcatgaggt cgtcgtagtc cttgctgctg gcgtcttcct tcttgccggt
    10381 ccagcggtct tcgaggatgc cgaggatatc cgtgaaggtc ttgaagacgc gcttgacttc
    10441 cgacatggct gccgaatcga cggggacgcc gttgttgacg acggcattct tgaagatgtt
    10501 gacgttcttg gccaaatcga acatggccgc gctggccagg gccgtattga agtcgtcgca
    10561 catagcattt tcaaagtctt cttcgtcctt gtgggccgtt tccaggagct gctgggcttc
    10621 cggcgattta gcaccggccg gcatagccag gagttccagg acgttgtcga tggccgtgcc
    10681 caggcgttcg aggctgcgcg tcatttcttc cagccgttcg tcgctgaagt ccaaggggct
    10741 gcggtagtgg ttggaaatga ggaagaagcg cagggcatcg gggctgtagt gttccaggac
    10801 gtccttgacc aggaagaagt tattcaggga cttgctcatc ttttccgagt tgatggtgat
    10861 gaagccgttg tggagccagt atttgaccat cggtttcttg cccgtaaagg cttcggactg
    10921 ggcgatttcg ttttcatggt gcgggaagat gaggtcgctg ccgccgccgt ggaagtcgaa
    10981 ggtatcgcca aggtacttat tgctcatagc cgagcattcg atgtgccagc ccggacggcc
    11041 tttgccccag gggctgtccc acgacggttc gccgggctta gcacctttcc agagggcgaa
    11101 gtccatggga ttgtgcttgc ggtcgtcgac ttcgatacgg gcgccggcca tcatgtcgtc
    11161 gaggctgcga ccgctcaatt cgccgtagtc ggcgaacttt tcgacgctgt aatagacgtt
    11221 gtggtcgatg acgtaggcat agcctttgtc gatgaggttc tggaccatgt tgatgatttc
    11281 atccatgtgt tccgatacgc ggggatactt gtcggcccgg cggatgtgga gcttgtccat
    11341 gacgtcgaag tacgatgcga tatagcggtt ggaaatggta tcccacgtga cgccttcgcc
    11401 gttggcagcg ttgatgatct tatcgtcgac gtcggtgaag ttctggatgt gcgtgacttc
    11461 atagcccaag tgttccagat agcgcttgat gacgtcccac gtgacgaaag gacgggcatt
    11521 gcccatgtgg gaatggttgt acggcgtaat gccgcagaca tacatcttga cctggcccgg
    11581 cgtaacgggc acgaagattt cttttttacg ggtcaatgta ttatatactt tcagttctct
    11641 catacggtaa cctccctgaa atttgaacaa aaaggccacc atcccctatg acagagacgg
    11701 tggcccgtgg ttccactctg tttttacggc agtgccgtaa tctttactgc gcagtaacgg
    11761 gcgcccccgg accggattac tcctttcacc cggcctgctc acaaaggcag cattgcagtt
    11821 ttatgacctg gaaaggctct cagccatgac ccttcttctc tgtcggatgc cactgcaact
    11881 cattttgatc atcgcagatt cttatttgat acatattatt agttaatacg catgtatata
    11941 gtacccaata atcgggctca tgtcaagccc tttatgccaa cgtttcgtcc agacgggcca
    12001 ggactttgtc gcggcccatg agggcgacga tgacgtttag gtccgggccg tgcatgacgc
    12061 cggtgacggc gacgcggata ggcatgtaga cgaacttgcc cttgaccttg aggcccttct
    12121 ggattttctt gaacaggggc tgtaccgtgt cgggcgtgac gtcgtcgagg gcagccagtt
    12181 catcgcggaa catcttgagg acggacggag ccgtttcttc tttgaggact gctgcggttt
    12241 cttcattttc cggcgcatag tcgtcggtga agaagacctt ggcagcgtcg acgatctgag
    12301 cgccgtactg gacgtgttcg cgcatggcgg cgcagatggc tttcaaccag gcagcttcct
    12361 tttcgtccgg attttcgctg gcataacctg ccttctgcag gtgcggcagg cagacggcat
    12421 agagctggtc ttcatcgagc tgcttcatgt aatggaagtt gatccagttg agcttttcga
    12481 tgtcgaagac ggccgggttc ttggcgacgc ggtcgagggt gaactgttcg atgagttcat
    12541 cgcggctgaa gatttcctga tcgccttccg gcgtccagcc gaggagggcc aggaagttga
    12601 ccagtgcgtc gggcaggtag cccatatcgc ggtactgctg gaccgatgta gcgccatggc
    12661 gcttgctcat cttcttcttg tcggcgccga ggatgagcga tacgtgagcg aactgcgggg
    12721 cttcaaagcc caatgccttg tagatgacca gctggcgcgg cgtgttggac aagtgttctt
    12781 ccgcacggat gacgtgggtg atgtgcatga gggcatcgtc gaggacgacg cagtagttat
    12841 agaccgggat gccgtcggat ttgacgatga cgaagtcgcc gacgttgttg gaatcgaagg
    12901 atacgtgacc gcggacgagg tcgtccagtt caatcgtttc gttcttcggc gtcttgatgc
    12961 ggatgacgtg gggacggcct tccttgaggt actgttcgac cgtttctttg ggcaggtcag
    13021 cgcatttgcc ggagtagacc ggcgtttccc ctttggccag ctgagcctgt ttttccgctt
    13081 ccagttcttc cggcgtgcag aagcagtagt aggccttgcc ttcggccaag agcttttccg
    13141 tatatttctg ataaatgccc aggcgttcag tctggcggta cgggccatcg ggaccgccga
    13201 cgtcgatgcc ttcatcccag gtgatgccca gccatttcag ggcttccttg atgttttctt
    13261 ccgattccga cgtcgaacgc ttgcggtccg tatcttcgat gcggacgacg aacttaccgt
    13321 ggttgtgctt ggcaacgagg tagttgaaca gtgccgaacg ggcaccgccg atgtggaacg
    13381 ggcctgtcgg gctcggagca aaacgaacgc gtacttcctg tgacataaaa aattcctcct
    13441 aaagactata tatatgtatt attgatagct aaggaccatg gctgccgcct gggcggccat
    13501 gccttcttcc cggccgacga accccagttt ctctgtcgtc gtcgccttga cggagacagc
    13561 agtcttgggg atacgcaggg cctcggcaat attagcgatc atctgcggca catagggctt
    13621 gattttaggc cgttgtgcca ataaggtcac gtcgaggttg acgatgcggc agcatttctc
    13681 ccgcagcaga tggcggacct tttccaagag gaccaggctg gaaatatcct tgaaggcctg
    13741 gtccgtatcg ggaaaatacg tcccgatatc gcggagcccg gcggctccca gcagggcgtc
    13801 catgatggcg tggatgacga cgtcggcatc ggaatggcca tcgaggcccc gttcaaaggg
    13861 aatctcgacg ccgcccagga ccaggcgccg cccttctttc aggcggtgga cgtcatagcc
    13921 cgtaccgatg cgctggactt ccctcccctt catgtacagg gacaggatgg gaatgtcgtc
    13981 gcgcgtcgtc accttgcggt tcttctcgct cccttcgagg atgaccacgg gtatacccag
    14041 ggcttcgacg agggatgcgt catctgtcgc ctggatgccc tcggtccggg cctgctcatg
    14101 ggcttccatg agcaggtcgc ggtgaaagat ctgcggcgtc tgggcggcga ccaggcggct
    14161 gcggttcagc gtatggagcc ggccgtcgtc gccgcggacc ttgaccgtat ccttgagagg
    14221 gatgccgtaa atggcagctc gggccgtttc catcatggcc gcgccctggt cataccagtc
    14281 cgggccggca aaaggccggg cgccgtcgtg gaccatgacc acatccacgt cgcggctgac
    14341 ggccatgagg ccgttgtaga ccgaatcttc ccgttcggcg ccgcccaggg cgtagcgtac
    14401 gggctggata ggatggaggt catcgacgag gcggcggatt tccttctcat cgacgcggga
    14461 atggacgatg accacttcac tgaccgaccg ggccgcgaaa atgtggcgca gcgtatgggt
    14521 caggacaggc tcgccgcaca agggatagaa gagcttgttc cgctcgtagc cgaagcggct
    14581 ccctgtgccg gccgctacga cgatgacaga tactttcaca catttcacct actcatttgc
    14641 aatcttaacg aatatcatgc gcccagccga cgtctgcagg acagacgtca ccgtaacggg
    14701 cacagtagaa ccgatgtatt tctggccatc ttcgacgacg atcatcgtgc cgtcatcgag
    14761 ataggcgacg ccctggttct cttccttgcc ggccttgacg atgaggacgt tcatgtgttc
    14821 cccagggatg cgggccggtt tcagggcatt ggccaggtcg ttgatgttga ggacggccac
    14881 gccctggagg gcggcgactt tattcaggtt atagtcgtta gtgacgactt tggcattgat
    14941 cttgcggccc aggcggacga gcttggaatc gacgccctgg acgtcgtcga aatcgtcgtt
    15001 gatgatgcgg acttcgacgt aattatcttc ctgcatctgt ttcaggatat ccaggccgcg
    15061 gcggccctta ttgcgcttca acaggtccga cgaatcagcg atgagctgca attcttccag
    15121 gacgaagacc ggcaccagca gcggcccttc caggaagccg gtactgcaaa tctcggcgat
    15181 gcggccgtcg atgatggcac tcgtatcgag gagcttgccg gaaaattcca tgtccttatg
    15241 cttgctgccg tgatccttgc cggacttgcc caggcgcaga ttgcctgcca gggcgatgat
    15301 ttcgctcttc ttgcggatgg cgatgctcat gccggcgtag ccgaagacga tgctgaagac
    15361 gataggcccg taggccccga tgatcggcag gcgtgcgaac gctaggccaa ttaaattcgc
    15421 aatgataagt ccaaaaagca accccagcgt cccgacgatg atgtcctggc tctcgaaatc
    15481 attcaggccc gattcgatgc ggtgaatcgc tgcccagatg aggcgcagca cgaacggcga
    15541 aatcccatag cccaccaggg ccccgacgat gatgccgacg atataggcta cgatggtgat
    15601 gatagtcgta ccgaaaatac tgccgtggaa aaattcccct tcgatttcgg cactgatgaa
    15661 gggcgtcgta atcacctggt cggcaatcat gacgaagatg acggccataa tcaggacgat
    15721 gacacctttt atgatgttat cggccacatt gcggccattg aacttcttcg atttcggccc
    15781 atcatgattc gattcatatt tatcttccat agagtaaaaa aacacctccc ctttgtatat
    15841 acttctataa ctatactagt ttaccacatt tcccgtcaat gtaccacagg tggcaggtaa
    15901 agtttttaca gtcgtcagat tcccctcagc gatgcatcat ctgggcaaac gtaacgagat
    15961 ggatatgctc atttcctgcc gcttcacgta agcatctttc agaaaaccca cgcttggcaa
    16021 aaatcatgta ttccttgcgt gacgcttgga aaagtccgcc ccgataaagc aacagttcca
    16081 gcacatcctg gtcaaccaat tcattccgcc atttacattc accaaagagc cgttctcctt
    16141 ccacattgcc cgctacgata tccagttctg tctgcttatg ggtccgcggg tcgccccccc
    16201 accagcgccc ggcatcatca aagacaaaag gcagacggcc gtcagcattt tcacgccata
    16261 accattgtag acaaatatct tcgaagacct ggcccataaa atccggcagg tgcggcttga
    16321 tccgctgcca ggccacttca gccatgccgt tttggatttg cgaatagtgt gtggggatga
    16381 aacgatacca aaagcggaac aggctgacct ttaacaaata aatactttta cgggaatgac
    16441 ttttacggga atgactttcc ccgaaaggat attctttctt gatgatgccg aggccgataa
    16501 gattcttcaa gtacacgaca caggcactgg cttccagccc cgttttcgtc gcaatctgac
    16561 tgactttcgt actccccgtc gcgacggcct ggataatggc attatacgta gccggctcac
    16621 gcacttcctg cttcaagagg ttggacggtt cctcgaagag ataagccata gggtccagga
    16681 a
//
LOCUS       DAWABZ010000027        16598 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_936_length_16598_cov_3.327691, whole genome shotgun sequence.
ACCESSION   DAWABZ010000027 DAWABZ010000000
VERSION     DAWABZ010000027.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 16598)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16598)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16598
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_936_length_16598_cov_3.327691"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2683..2692
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3803 a         4957 c         4740 g         3088 t
ORIGIN      
        1 ctgtcttaat gcgacagcct ctttttttgt atccatccta ttcatgcgtg ctgttttgtg
       61 ctacaattaa aaactatata agcagcacct ctgtagtagg ggcttgcacg tggcaagccc
      121 gctccaggat atgagcgatt ttcccgttta gggagagtgc cgcccagtat ccagcaggtc
      181 gcccgcgtgg cgacccctac ggtgcaaaaa gggatatttt ttgttacaat ttaaaactat
      241 ataagaagag ggaaagaggg gtttacgatg agtgcaatta aaacggaact ggcagctttt
      301 atcgctgccc atacgagtga ggattttgtc atgacgccga ttaccatgcg cgaagcgtcg
      361 ctgcatacgg catgggaaca agagctgccg aaagtgccga tggcatcggt cgtcgatacg
      421 gtattggacg gctgtgaggt ctacgacgtg ccggtccgca tctatattcc cgatacgagc
      481 cggcccctgc cggtcctgat ttactatcat ggcggcggtt ttgccatcga cacggtcgcc
      541 gtctatgacc cggtctgccg ccgcattgcc caggcaacgc agcatatcgt catttcgccg
      601 gaatatcgcc tggctccgga aaatccttat cctgcggcct atgaagatgc cctggccgta
      661 gcccggaagg ccctgcctgc gctcgatgcc ttgcagatcc cctaccaggc ggacctgacc
      721 ttgtgcggtg acagcgccgg cggctgcatg gcagcttata cgagccaggc cctgcagcat
      781 gatgcatccc ttccgctgac tcatcagatc ctggtctatc cctgcctgga tatgacccac
      841 tccttcccgt cggtccagga gaactgcaat acccagacgg gctttacgcc ggccaagatg
      901 acctggtatt tcaaccagtg tttccgtccc ggtgacgacc gccgggatgc ctcgcccctc
      961 tttggcgaat tgtcggcaga tatgccggcg acgctggtct tgacgacgca gttctgcccc
     1021 ttccgcgacg aaggccgcga ttacgtgcgc cgcctggctg aaaagggcgt gcgggccgaa
     1081 acgtataatt acggcaacat ggtccattcg tacctcaact ttgaaaaaat atgctacgac
     1141 gaaatctgcg acacctaccg gcgcatggcc gattttttgc aagactgagg gaaatggctt
     1201 atactagaag catgtactta cgctaagtaa aggatgtgta ttttatgaag aaatgccaac
     1261 aatgcggggc gcccttgcag gatgacgacc gcttttgtca ggcctgcggc gctgaacagc
     1321 tgccggaagg ggagccgaag aaacgcattt gtccggaatg tggcaaatgg gtgagcgtcg
     1381 atgggaaatt ctgcccattc tgcggtgccg aactgccgaa aatggatgat gacgaaccgg
     1441 tcatcgacga agcgacggga acggaaacct ttgtccataa gcaccgggaa ccggtccggg
     1501 acgaagagcc gcagcggccg ccgcagccga tggaacgacc ggtcatgacg ggccggccga
     1561 cgccgccgcc ttttcaggaa aagccttttt acgtgcgcta tcagaaggcc atcatcgccg
     1621 gtatcgtcgt cctcgtcctg gctctggccg ctgccgtggc ttttcacttc tatggcagcc
     1681 gcagcagcgg ggactatgtg aaggatgccg tttccctgtc gcagcagctg gcgtcggaaa
     1741 atgaatctgc caccagtacg gccaagcgcc tgagccagaa gagcaaaggc gacgatgtcg
     1801 attccctggt caaggatttg ggcaagagcc gcaagaacgt cgaaaagatg gccaagaatt
     1861 atcagcccaa gaccattccc gaccagtatg ccgccgatga caaggctatc cgcgaagtca
     1921 tgcgcgacca gatggccatg tatgacacgg ccatgacgat tgctgaaaat ccgtcagacg
     1981 acgctatcag cgaaaaattg gatgacctgc gcgacgaagt ccagaaggcc aagaagacgg
     2041 cgcggaccat caaggtcccc ggtgccgatt tcacagctgc cgtcgatggc gacgacctgg
     2101 tcagctacct ggaaaaatac gtcagcgtcc agaccaagcc cgatgaaaag ccgcagcctg
     2161 aagccaaggc caatccggat aagcagatcc tggcgtccta tgccagcagc aaggacttgt
     2221 tcaacgacga tatcggcaaa ttggccggcg acatcaacgc ctacttgaat gaccacacgg
     2281 acttcaaggg caatcgcgaa ttcaccagcc gggccaatac gctctacgtc cagatttcca
     2341 agacccgcag ccagctggac aacgatacgt ccatcgacaa tacggccatg aaatataagc
     2401 tcgatgccct cttccaggcc gaacaggacc gcgtccgcgg cctctacgac ggcgtcagcg
     2461 ccagccagcg cggacaggac taccgcccag gcttccagcg gggccaggcc gcatcgaacc
     2521 gctacgataa actcaacgac gaatggaata gtttgtaatg ttcgtggccc ctacggctga
     2581 atggtgaatc acccatatcc cggggcgggc gccccgcgtg gtcgccccta cagaaaagct
     2641 ttaaatttaa agccatctgc taacccctaa aaaggagctg tcnnnnnnnn nncactcatt
     2701 tcttcacttt tttcatggac aggccgatgc ggccgcgggc ggcgtcgacg ctgatgattt
     2761 cggcttcgac gatgtcgccg gcgtggacga cgtccgtcgg atggcggaag ggatgccggc
     2821 tcagctggga gcggtggacc agtcccggcg tcttgaggcc aaagtcgacg aaggcgccga
     2881 agtcgacgac gttctggacc gtgccgcgga cgacggagcc aatctgcagg tctgacaggg
     2941 acaggacgtg gcggcgcgtc agcggtttcg ggaagtcgct gcggacgtcg cggccgggct
     3001 tgcgcagttc ttccaggatg tctttgatcg tcggctcacc ggcgccgaga gaggcggcca
     3061 gtttgggagc ggcgttcatc tggatcttat cgcgcaggat ggctaatttt tccgggtcct
     3121 tgagctcgtc atggctcagg ccgtagtggc cggctatctt ttcggccagg ccgtatgatt
     3181 cgggatggac cgacgtgttg tccaggggct ccgtcccgtc cttgatgcgc aggaaaccgg
     3241 cgcactgggt gaacgtcgcc gggcccaggc gggggacgtt gagcaggtcc tgccggttct
     3301 ggaaggggcc gttctcgttg cggtaggcga cgatattgcc tgccgtcgcc gccgtcaggc
     3361 cggcgatgtg ctgcaagagg gccggcgatg ccgtgttgag gtcgacgccg acgtagttga
     3421 cgacagacgt gacgacctgg tccagggcgg ccgacagggc tttctggttg acatcgtgct
     3481 gatactggcc gacgccgatg gcccgcgggt cgatcttgac cgattcggcc agagggtcct
     3541 ggatgcggcg ggcgatggaa acggcgccgc ggatggtgac gtccaggtcc ggcagttcct
     3601 cccgggccag gtccgatgcg gaatagacgg acgcgccggc ttcgttggcg atgacgtagc
     3661 ggcatttcag gtgataggct tcgatgagat gggatacgaa ttgttccgtt tcatacgagg
     3721 ccgtcccgtt gccgatggaa atgagggtga cgccgtggcg gcgtatcatg ttggccaggc
     3781 tgacggcact ctggtgtttc tgctgttccg agtgggtcag gtagtacgtg ccgtagtcga
     3841 ggacctgtcc cgtcgcgctg atgacggcgg ccttgcagcc cgtccggaag cccgggtcca
     3901 agcccaggat gatctggccg gcaaagggcg gctgcagcaa gaggtggcgc aggttttcgg
     3961 caaagacgtc gatggcctgc ttttcggcct gtgccgtcag ttcgttgcgg atttcccgtt
     4021 ccatctgcgg cgaaatgagg cgcttgtaac tgtcggcggc ggcgtcgcgc aggatctgat
     4081 catacggcga cggcctggtg atgaccagct ggcagagcat ggcgatgtgg cggtctgccg
     4141 gctccttcaa ggtgactttc agtttctttt cgttttcgcc gcggttgatg gccaggatgc
     4201 ggtagctcgg gatacgggcg acgcgttcgc tgaagtcggc ataggtcagc atgggaccgg
     4261 cttggtcttc ttcgacttgc aggctgcagg tcaggcgggc ctgctgccac agttcgcggc
     4321 gcaggtggcg gcggaaatcg gcccgttccg aaaagcgttc ggccaggatg ttggctgcgc
     4381 cctggatggc gtcttcgacg gtcggtacgt catcgctgag aaaggccagg gcggcgtctt
     4441 ccggagccgg gcccttgggg tcctggctgg caaagcagtc ggccagcggt tccaggccgg
     4501 cgtcgcgggc catggaggcc ttcgtccgtt tcttgggttt atagggcagg tagaggtctt
     4561 cgacgtcctg gagctgccgg gccgcttcca ggctccgtgc caggtcgtcg ttccagcagc
     4621 cctggtccat gatggactgg cggaccgttt ctttgcggct ggccagggcc tgttcgtatt
     4681 catattgctc ctggatgcag cgcagctgta cttcgtcgag ggtccctgtc gcttctttcc
     4741 ggtagcgggc gatgaaaggg atggtattgc cctgggcaaa gagggaaagg gcagattgga
     4801 cttgcgccgg gcggataccc agagtcctgg cgatttcagc gatcatcgtt tcctctgtca
     4861 tgattacatt ccttcctaac aaaatagtta atattactta ttgtataaga agaatttcgc
     4921 gtaaaacatt gaaatttttc ttaataaccg ttacaatata atacaggata ctctccagta
     4981 ttctatatga ttcaaagcta gaataaaact agaatgtctt attataccac agttttccaa
     5041 gaaaggggga taaaaatgga aattgctgaa gttttgcgaa aaaacggcta taaagtcaca
     5101 ccgcagcgcc tggctgtgta cgaagccatt aaccacaatc cgacgcatcc caatgccgag
     5161 gcgatttata agatattgca gcccaattat ccgtccatga gtctggccac ggtctataag
     5221 accatggaaa tctttgccaa aatcggcgta gtccaggtat tgcaatgtgc cgaagatgcc
     5281 catcgttatg attataatac gacgcctcat gcccatatcc gttgtgaaaa gtgcaaccgc
     5341 gtcatcgata tcgacatgga ccaggagggg ctgcgtaagg ccgctgccga acagagcggc
     5401 ttcgtcgtca acggcgtcag tatttcgttt gtcgggattt gcccggaatg tcgggaaaaa
     5461 tcgtaagtat ttgattttca tatagtctaa ggataggggc tgctgcatga ggccggacgg
     5521 gcttcgtgcg gcagcctttt tttgttatcc gtcgcgcagg aggaaaaaat atgttgcata
     5581 ttggatgtca tttatcggtt tcacaaggat ttttgcacat gggtgacgaa gctttatcta
     5641 taggggcaga tacgttccag ttcttctccc gcaatccccg cggcagcggc gtccgcaaac
     5701 tggacgtcga cgatatacag gccctgaacc agctcatggc agcccatcac ttcgcgccca
     5761 tcctggccca tgcgccgtat accctgaacg gctgctcgaa gacggccaag acgcgagatt
     5821 tcgcccgcca ggccatggcc gacgacctgc gccgtctgga atacgtcgac aactgccgct
     5881 ataatttcca tcccggcagc catgtcggac agggcgctga aacgggtatc gccctcattg
     5941 ccgacatgct caacacctgt atgttccccg gccagaagac gacggtcctc ctggaaacca
     6001 tggccggcaa ggggacggaa gtgggccgga cctttgaaga actggcggcc atcatcgaca
     6061 aagcggccat gaacgataaa atcggcgtct gcctcgatac gtgccacgtc tttgacggcg
     6121 gctacgatat cgtccaccat ctggacgacg tattgacgac ttttgataag gtcatcggcc
     6181 tggaccggtt gaaagccgtc cacgtgaacg atagcaagaa cgagctggcc agccacaagg
     6241 acaggcatga aaaaatcggc cagggccaca tcggcctcga agccttccgg gccatcgtca
     6301 atcatccggc cctgcgggac ctgccctttt atctggaaac acccaacgat atggcaggct
     6361 atgcccggga aatcgccctg ctgcggtcgc tgcagcagta gcttggtaag gaggtattct
     6421 atgcgtatct tacatacagc cgactggcat ttaggaaaat tattctatgg gaactatctg
     6481 accgaagacc aggcctacgt cctggagcag cagctcctgc ccatgatacg ggacgagggc
     6541 atcgacgccg tcgtcctggc cggcgacgtc tatgaccgca gcctgccgcc ggccgaagcg
     6601 gtggaactct tcgacgacgt ggccacgcgg atcacggcgg atttgcacgt ccccttcctg
     6661 gtcatcagcg gcaaccacga cagcccggcc cgcctgtcct ttgccagccg cctcctggcg
     6721 ccgcagggcc tctatatggc cggtgaactg gagcgcctca cgggcccggt catcctggaa
     6781 gatgaggccg gctcggtcgc cttcctgacc gttccctatg ccgaaccggc cgtcgtccgc
     6841 cagtgcctcg gccaggacga ggtccgtagc catcagcagg ccatggaggc cctcctcggc
     6901 tggcagggca gccaggtcac ggacggcgta cgcacggtct gcgtcgccca tgccttcgtc
     6961 gccggcggcg tagccagtga ttcggaacgg cccttgtcca tcggcgggac ggaccaggtg
     7021 acgagcggcc tctttgcgcc gttcagctat acggccctgg gccatctcca cgggccgcag
     7081 aaagtcacga gcgataccat ccgctatgcc ggcagcctgc tcaaatactc gttcggcgaa
     7141 gcgcgccagc ataaaggcgc cgtcatcgcc gacatcgggc ccgacgggaa agtggacctg
     7201 gccttccggg atttcgtgcc gcgccacgac gtgcgcatcg tcagcggaac cttcgaggcc
     7261 atcatggcgg ccgatgacga caggccggac gacttcatcc tggcccgcct cgacgacagc
     7321 cagcccatcc tcgacggcat ggcccgcctg cggcgcaaat accccaatgc catggcgatg
     7381 gaaatgccca accgcaatac ccgggccgca ggacagcgcg atttcgacgt acagcaggtg
     7441 acggaacagg aactgttcac cagcttcgcc caggccatgc ggcccgacca gcccctgacc
     7501 gaagccgagc aggactgcgt ccgcaccttg tgggatgaat tagaaaaggg aggcagcctg
     7561 ttatgattcc gatttactta gaaatgacgg cctttggccc ctatgccggt caggaaatcg
     7621 tcgacttccg cgaactgggc gaccataaac tcttcctcat ctatgggccg acgggttccg
     7681 gcaagacgac catcctcgat gccatgtgct atgccctcta cggcagtacc agcggcgaca
     7741 gccgcactgg cgcccacatg cgcagcgaat acgcgccgcc tcaggaagcg acggccgtgt
     7801 ccttccgctt tgccatcggc cggaaatact accgcatcga ccgcagcccg gaacaggaaa
     7861 tcgccaagaa gcgcggcaca ggcctgaaga aagccgcggc ccacgcggcc ttatatgaaa
     7921 cggacgaaga cggggcccag gaacagcttg tcgccgccaa gaacgtgacg gctgccgtcg
     7981 aaaggctgct gggcttccag gccgaccagt tccgccaggt cgtcctcctg ccgcagggcg
     8041 acttccgcaa gctcctcctg gccggttcca gtgagcgcca gcagatcatg cagaccctct
     8101 tccacaccca gcgctacgcg cggctccagg agctggccaa ggcccgccac gacaccctgg
     8161 ccggccagta tgagcaggta cgccagcggc gggaacagtg cctggccgac ctgggcatcg
     8221 ccagcgaaga agagctgccg gataaagaga aagcctggca ggataaaagc gccgccgtcg
     8281 atgcacagtg gcacgaggct gaaaccaggc gccagacctg ccagtctgcc ttccaggccg
     8341 cccagacctt ggaaaatcac tggctcctgc gggaacagaa gcgccgggaa gcggcgcagc
     8401 tccaggacca ggccggccgg ttccaggaaa aacgggatac catcgccatc ctgcgccagg
     8461 ctgaaatcct ggccgaaccg tgccgtcacc tcgacgacct ggaaaaagag ggcaaagagg
     8521 ctcataaggc ggctgaaaaa gcccgtaagc tggccgagaa ggctgagaag gacctggctg
     8581 aagcccagcg gcagctggcg gccctttcgg cccgtgagag cggctataag gcagcctgcg
     8641 aagaacgggt ccagctgacg ggcctgcagg aaaaagccgg ctcctaccgg gactgctgcc
     8701 tggaagaaga acggtgccgc aaggcggcag gcgaagctga acggcgctgg gaccaggtgc
     8761 ggcagaaaaa ggaagcagaa aaacaggccc tcgacaaggc ccgggaagcc gtcgccggcc
     8821 tggccgaaca ggccgtagcg gcggaacagg ccaaagcccg ctggcaggcc ctgagcgacc
     8881 ggctccagcg cgaaggccag gccgcttccc tggaagaaca gctgaaagaa gccgggaaac
     8941 aatggcagca cagtcagaac ttcctggaaa aagccgctgc caaggcacgg gacgaacgcg
     9001 tggcctatga aagtgtccgg gccctgttcc tccaggccca ggccgccgtc ctggcgtcgg
     9061 aactggaaga agggaaaccc tgcccggtct gcggctcggc ggcccatccc atgccggccg
     9121 tgcgcccgga ttccctgccg gaacggcgcg acgtcgaaca gcaccagaaa cgggccgacg
     9181 ccagcgaagc caagcgacag cagtgggaag tgacggccaa agccgatgag acccgccatc
     9241 aggctttgaa agagcagtac gacgccctgc gccgccagta cccccaggat gggaccctgg
     9301 ccgactggca ggacaaggcc gaacaggccc ggaaagccat ggaaggcctg cagcagcagt
     9361 tacaggccgg tgaagccctc aaaaaggccg tcgccgccgg ccaggcccgg ctgagccgct
     9421 gggaaaagga cgaagaagcg gcccggacgg cagccgaaca ggcgcggacc cttgcggcca
     9481 aggcggccga aacgaaggcc cgcctggaag cggacctgcc cgaagcctat cgtgacctga
     9541 aggccctgca gcagcgcctg gatgccttgg gcaaggccat tgcagcctac gaaaaggact
     9601 tgcagcagta ccgcgacgct gtcgtcgccg gcgaacgcca ggctgccaag cagcagcagg
     9661 aagcgcagaa ctggcagcac caggtcgaag tgctgcggga ccgcttcagc cagggggtcc
     9721 aggccctgcg ggaccgcgtc gtggcagccg gtttcgagag cgtcaaggcc tgccgggcca
     9781 tgcaggctgc agtcggccag atcccggaac tggaagcggc cgtccgccag tacgacaccc
     9841 aggtccagca ggtacagggc cagctggacc aggaagaaaa ggccatcggc agccagcacc
     9901 ggcccgacgt ggcagcagcc cgcacggcgt taactgaagc cgaaaaagcc agccaggcct
     9961 taggcgaaga aaagggcagc ctggccgcac agtaccggcg ctggcagcag ggcaaagcgg
    10021 ccttggccgc cctgcagcag gaaggccggg acctgaccga agcttataaa accgtcggcg
    10081 ccgtctacga actcatcgcc ggcaagcaga cgggcatcaa ttttgaacgc tacgtcctcg
    10141 gcgccctcct cgacgaagtc ctggccgccg ccaacggccg cctgcagggc atgagccgtc
    10201 agcgttacag cctgcagcgc tcccggtcgt gggacgataa gcgggtcaag cagatcggcc
    10261 tggacatcga agtctatgac aattacacgg gctatgcccg gccggccaac accctgtccg
    10321 gcggcgaaac cttcctggcg tccctctccc tggccctggg cctggccgac gtcgtccagg
    10381 cgtattccgg cggcatccac ctggatacga tgttcatcga cgaaggcttc ggcacccttg
    10441 acggtgaaac cctggattat gccctgaaga ccctggcgtc cctgcgcagc ggcggccgcc
    10501 tggtcggcat catctcccac gtaccggaac tgaaggaacg catcgacgcc cgcctggccg
    10561 tccacaagac cgaccggggc agtacgtcgt cctttgagtt tgcctgagcg ccgtgattag
    10621 tatataatac gattataacg aattgagagg gggagcaacg tgtctattca aaaagtacgc
    10681 gactattttg cccggtgggg gatggcagac cgggtcatgg aactcgacca gtccagtgcg
    10741 actgtcgaag aagcggcggc cgcccttcat acggagggcc ggcgcattgc caagtccatg
    10801 tctttcctca tcggcgacca tccgatcctg gtcctttttg ccggtgatgc ccgcgtggac
    10861 aaccataagt tcaaagaacg gttccacaag aaggcgacca tgatcaaggc cggcgacgtg
    10921 gaacggctca tcggccatcc cgtcggcggc gtctgtcctt tcgccatcaa cgaaggcgtc
    10981 gacgtcttcc tggacgaatc gctcaagcgc ttccagaccg tctttccggc ctgcggcacc
    11041 gccaacagtg ccatcgaact gacactgccc gaactggaaa cctattccca tgccaggaaa
    11101 tgggtcgacg tatccaaggg ctggcaggaa gagaaccaat aaataaaaag ggggtagtac
    11161 tatgcaattc acctttaagc acaccaattt caacgttttg aatctccagc gcagcatgga
    11221 cttctacaaa gaagccctgg gcctgaccga agtcagccgc atcgagaacc cggatttcac
    11281 catcgtctat ctcggcgacg gcgtgtccga cttccagctg gaactgacct gtctcaaaga
    11341 ccgcaaggaa ccgtataacc tcggcgaaaa cgaattccac acggcctttg ccgtcaagga
    11401 tatggatgcg gccctggcca agcacaaaga catgggctgc gtcgtctatc agaacccggc
    11461 catgggcatt tatttcatcg tcgaccccga cggctactgg ctggaaatca ttccggagcg
    11521 ctaggccatg aagaaagaat atacccagcg gacagcccgc ctcatcggcc aggataaggt
    11581 ggacgaatta gatacgaaaa aggtcctcat cttcggcgtc ggcggcgtcg gctcagccgt
    11641 cgtcgaagcc ctgggccggg ccggtgtggg ccatctcatc ctggtcgatg ccgacgtctt
    11701 cgacccgtcc aacgttaacc gccagctcgg ggcgacggtc cagaccattg gccggccgaa
    11761 agttgaagtc atgaaggaac gcctcttgtc catcaatccc gacatcgacg tcgagaccca
    11821 tgcgacgttc tatctgcctg aaagccatca gggcttcatc gcccattccg gggtcgatta
    11881 cgtcgtcgat gccgtcgata cgatgagcgc caagatcgcc atcatcgacg aagcctatca
    11941 cagcggcatc cccgtcgtgt cctccatggg ggccgggaat aagctccatc cggaatattt
    12001 ccagctcgat tacatcgaaa agaccagcgt cgatcccctg gccaagatca tgcgccggga
    12061 actgaagaag cgccgtatcc gccgcatcaa ggtggcctat tcgacggaag tcccgcataa
    12121 acccttctcg gacagcgatg aagtgcgccc cagtccgggc agcatttcct ttgtcccgtc
    12181 gacggcgggc atgatcctgg ccggtgccgt cgtgcgcgac ctcctgaatc tggattaaaa
    12241 gaaaattttt gctagttata cacaagggct gcgtctataa aagcgcagct cttgtgttgt
    12301 cctttcaagg cctgccagcc attttgataa aaactaacct taacttgtcc acaacatgat
    12361 ggagtatgta cacgttgaca ttcgctacta tatatagtat catagggcat gtgaaaaacg
    12421 gaccctgtac catatatctt gtagcaggta tgggaaaatg tacaatttgt atactatata
    12481 ctgtggaggt agacatatgt tagaagtaat caagcgcaat ggagaacgag tgcccttcaa
    12541 tcgagataag atcaccgtag cgattgaaaa ggccatgaac agcagcagcg gtatttacga
    12601 agaagggcag gctgccaaga ttgccggtga aatcgaagcg tatgctgctt ccatccagaa
    12661 ggacctgacc atttacgcca tcgaagacca ggtctactac cgtttgttga aatacaacaa
    12721 tccggccacg gccagggctt acgaaaacta caagaccatc caggccttca agcgccagac
    12781 caatacgacc gacgacgatg tctttggcct gctgaacaac accaacctgg aagtcctcga
    12841 cgaaaactcc aataagaacg gccacgtcgt atcgacgcag cgcgacctca tcgccggcga
    12901 agtgtccaag gacattgccc gccgcaaact gatcccggcc gacatcgtcc aggcccacga
    12961 cagcggcgcc atccatttcc acgacatgga ctacatcatt cagcccatgt tcaactgctg
    13021 tctcatcaac ctggaagaca tgctggccaa tggtacggtc atcaacggca agaaaatcga
    13081 tacgccgaaa tccttccagg tcgcctgcac ggtgacgacg cagatcatcg cccaggtcgc
    13141 cagcggccag tacggcggcc agtccatcaa cggcatcgac cgcatcctgg caccgtacgt
    13201 ccgcaagtcc ttcaagaagt acatgaaagc cgtcgtcgaa gaacagcgcg aaatctacgg
    13261 catcgaaccg gatatggaaa aggccgaaga aatcgcctgg aagcggacga agaaggaaat
    13321 caaggacggc atccagacca tccagtacca gatcaacacc ctcatgacga ccaacggcca
    13381 ggcaccgttc gtcacgctct tcatgtattt ccagccggat tacgaatacg ccagggaagc
    13441 ggccctcatc gacgaagaat tgctgcgcca gcgcatccag ggcatcaaga acgaagccaa
    13501 cgtctacgtc acgccggcct tcccgaaact catttacgtc ctggacgaac acaacgtccg
    13561 cgaaggcagc ccgtacttct atttgaccaa gctggccgct gaatgtacgg cgaaacgcat
    13621 gtatcccgac tatatttcgg ccaagaagat gcgcgaatcc tatgaaggca acgtcttcag
    13681 ccccatgggc tgccgctcct tcctcagccc ctggaaagat gaaaagggca actaccagtt
    13741 cgacggtcgc ttcaacatgg gcgtcgtctc cctcaacctg ccgcagatcg gcatcctcgc
    13801 cgacggcgac gaagaaaaat tcttccagat cctcgacaag cgcctggaac tctgcaaaaa
    13861 agccctgctc ctgcgcgtcg acctcctcaa gcgcatcacg tccgacgtat cgcctatcca
    13921 ctggcagtac ggcgccattg cccgcctgaa gcccggtgaa accattgaaa aattcatcta
    13981 cggcggctat gcgaccttgt ccctgggcta catcggcatc tacgaagcga cggtactgac
    14041 caagcactgc tcgcacacgg cgccggaagg ccggaaattc gccatgcgca tcatggacga
    14101 cctcaacgcc cacattaaga agtggcggaa ggaaacgaac atcggcttcg ccctctacgg
    14161 tacgccggca gaaaacctga ccaacctctt ctgcaaaatc gacaaggccc gcttcggctc
    14221 catccccgac gtcaccgaca aaggctacta taccaacagc tatcacgtcg acgtccgcga
    14281 accgatcaac gtcttcgaca agttctcctt tgaagccgac tttgaagaca aatcgacagg
    14341 cggctgcatc agctatgccg aaatccccaa catgacccat aatatcccgg ccgtcctgac
    14401 catggtccag tacatctacg accacatcac gtacggcgaa ttcaatacca aactggacta
    14461 ctgccatgaa tgcggctttg acggcgaaat catcctcaac gacgacaacg aatgggaatg
    14521 cccgcgctgc cacaacaaag accgcaacaa attgaccgtc atccgccgga cctgcggcta
    14581 tttgggtgaa aatttctgga acgaagggcg gacgcaggaa atcaagaacc gcgtcctcca
    14641 tatctaagag gtgacttgac ttgcattatg cacagatgcg gcaatatgac attgccaatg
    14701 gaccgggcat ccgctcgacg attttcgtca ccggctgctc acgccactgc ttcaactgtt
    14761 tcaacccgga ataccaggac cccagggccg gacaggaatg gacggacaag gaaatggaac
    14821 agctcctgtc ctggctcagc gccccgacca acagcggcct gaccctcctg ggcggggaac
    14881 ccatggaaaa caccgacggc ctgacgcaga tcgtccaaaa cgtcaaacaa gccatgcccg
    14941 ataaatcggt ctgggtctac tcgggcttcg cctatgacga aatcatccag gacccgcaga
    15001 aaaaagccct cttggacgtc tgtgacgtcc tcgtcgacgg ccccttcgtc gacgacctga
    15061 aagacccggg cctctacttc cgcggttcct cgaaccagcg cgtcatcgac ctcaaagcga
    15121 caagaaccag cggccagatc accctggtat ggcccgacgg ccgctgatga caaataatta
    15181 ataaaaatta tccgttgata ttatttacaa tggaagcctt tcctgctata ataactgtag
    15241 gggcgaccac gtggggcgcc cgccacagac gtgtggtgct atccgttacc gtataaatgg
    15301 aatgctttgc ggtctgccag gctggcgatt ttgttcgacg gccttgaatt tcaaaaaagg
    15361 agggcttctc atggttgttg aatcggtgaa aaaagttaag aatatcgtct ctgaagtctt
    15421 taaaggctat gccagcaatt ccagcggcgg ctgctgcggt gtatcccttc caccggaaga
    15481 acagcgccag ctcaaacgct tgaaggaagg ggcccaggcc gaaaagaaag attgacaggg
    15541 gctgtgaaaa tggctacact aaggatataa gaacaagacg cacgacgggg acaccaccta
    15601 aggtgtcaga cgtcgtgcgt ttttattttg ttacgagggg tgtataccat gcagttagta
    15661 ttgaacggaa agaaaatgaa ttgtccctgt gaccacttgg aagacctgaa agccgcctat
    15721 ggcagcggcc aggaaatcac tatcgtcaac ggctttgcca cgacagagaa cttagccctg
    15781 aaagagggcg atgaaatcta tttcatcccc aaagaccgcc tgccgccgaa agaagccctg
    15841 gaaggcatga tgtgttcccg ccatacaccg aaagtccacc agaaagtcag cgccggccgc
    15901 gtcgccatct gcggcctcgg cggcctgggt tccaatgccg ccgtctacct ggcccggacc
    15961 ggtgtcggcc atctgcacct catcgacttc gataccgtcg atgccagcaa cctcaaccgc
    16021 cagtcctaca tggtccgcga cctgggccag cgcaagaccg acgccctggc ccggcagatt
    16081 gccgacatca atcccttcat cgacgtccgc acggacttcg tccgcctcac cgaagacaac
    16141 gtgccgacca tcctgaaaga cgaccaggtc atctgcgaag ccttcgacaa tcccgatgcc
    16201 aagaccatgc tcgtccacac catcctggaa caatgtcccg ataaatacat cgtctccgcc
    16261 tcgggtatgg ccggctacgg cgacagtaac gccattgaaa caaaaaaaat caccagccac
    16321 ttctacatct gcggcgacca gacaaggaac gccaaacccg gccgcggcct catggccccc
    16381 agagtctcca tctgcgccgg ccacgaagcc aatttgatcc taaaactcct ggtcgacgtc
    16441 ttataaatgc ctctcctcat aggagaggtg gcccgaagag ccggagaggt ttattggtag
    16501 cacggagttc gtggtttgta gaagatggtt ttaaattttt tgtcatttgc tgcaaactaa
    16561 aaactacaaa cttaaaactc aaaaagaggc tgtcttaa
//
LOCUS       DAWABZ010000028        16503 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_946_length_16503_cov_3.536296, whole genome shotgun sequence.
ACCESSION   DAWABZ010000028 DAWABZ010000000
VERSION     DAWABZ010000028.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 16503)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16503)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16503
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_946_length_16503_cov_3.536296"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2827..2836
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6369..6381
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7089..7166
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3963 a         4519 c         4386 g         3534 t
ORIGIN      
        1 tttttcagta gggggaagac ccctatttat gtaaactatg ttattgcaca taacacacgt
       61 agggcgcccg ctttcgtata tgtgggaatt tcgtacagtg ccaggttcgt gatatgtgat
      121 atgtggggcc gctgcatgat ggtttatgct atcatgcggc ggcccctttt tgctttatat
      181 tttgtagtgc ggagtttgta gtttgcagag aaaaccttta aaagccattc gctaaccact
      241 aaccactaac cactagccac ttaaaaggag ctgccgcatg tgcgacagct cctttttggc
      301 tcccatcata ggggagctgg ccgccaaagg cggtctgaga ggttggtacg aatcactagt
      361 tccctaatat tctggcggca tgttcttttt cgtatgcggc atggagaggg catcgccggg
      421 atactggtgg tatttgacgg gtgatgtcgt gaagccgcgg cctacgactt ccgaggcgtc
      481 gctgatgatc atgaaagcct gggggtcgag tttgttgacg atttctttga ctttggccac
      541 ttcggtcagt tttatgacgg cgtagatgac ccgtttatcc tgcatggtat aggcgccctg
      601 gccgtggaga taggtggcgc cgtggccgac gtagcgcatg aggacctggg cgatcggcgt
      661 ggcccggttg gaaatgatga tgacgctttt acgctgtttc aggccgatga cgactttgtt
      721 ggtaatgaag gcggcgatgt agatggcgac gaaggtcagg acggctcgtt ccagggagaa
      781 gagccaggct gcggccagga ggatgaccag attgagaatc atcgtgaccg tacccatttc
      841 cagggaataa tatttcttga cgatggcacc gatgacgtcc aggccgccgc tgttgccatt
      901 gtatttgtag atgatgccga agccgatgcc cgacaagata ccgccggtaa tggatgacag
      961 catggcgtcg tgcatgatgt tccacgtcga caggaaggac gtgacatcga ccaggaccga
     1021 gagcatgatt gtaccgacga tgcttaggac tgtatagcta cggcccatga atttgtagca
     1081 ggcgatcatg atcgggatgt tgaggatgaa gatgccaatg ccgacgggga ggccggtcag
     1141 gaagtagaac atgatagcta ccccgctgag gccgctgctc aataagtgga aggggatgaa
     1201 gaaggcattc atgccgatgg cgacgaccag actgccgaca aaggacgcca ggtaggggac
     1261 ggctaattcc caaagggtag gtgaagtcag ggttttcata attttttttc ctccgtgttg
     1321 aatgaagaat tgtaaagtaa aggaaaaaac ggtataatca ttatagtata atgatgaaag
     1381 tgtgcattca cagaggatgc gcaaagggcg ctgttctctg ctgtcgtctc attataacat
     1441 agggaaaatt ttaatcaata agacaatgca cattttatgc acggagtgaa agtatgatga
     1501 atatagtaga aaaaggatat gggaaaatta atctggccct ggccataact gaccgtcgtg
     1561 ccgatggtta tcacaatatt gatactatct ttcaatcgat aggcctttgc gataccgtga
     1621 ccgtgagcga agccgatgcg ttcgagctca cttgttcgca gccgtcgctg gcctgcgacg
     1681 aaacgaatct ggcctataag gcttggaagg cactttgtcc gtataaaaag gacaatcaac
     1741 aagtgaccat ccatatcgaa aagcatatcc ccatcgccgc cggactggcc ggcggcagta
     1801 ccgattgcgc cgctgtcctg cgcggcctca accgcctctg ggggctggag ctgccagaaa
     1861 cagaactctg ccgtctcggt gccggtctcg gtgccgacgt gcctttctgc atctgtggcg
     1921 gcaccatgcg cggcaccggt atcggcgagg aaatgaaacg cctgccgccc ctgccggcgt
     1981 ggtccgtggt catcgtccat ccccgcgtgc ccgtacagac caaacaggcc tatgccctgt
     2041 tcgacagccg gatccagacg gcgcccgtcg ctgtcgatgc cgtcgagcag gccgtccggg
     2101 ccggcgattt ccagggattg acggcggcca tggggaatac ctttgaggaa ctgatcattc
     2161 ccaatgtgcc gcaagtcgcc gattgtcagc agcgcctgac cgcgttaggc ctgcagccgc
     2221 tcatggccgg cagtgggccg acggtgttcg ccctggtccc gcctggacag gaagagaccg
     2281 tccgcagcca gacggcgtcg tggcatgatg tcgacgtata catgacggcc atagtgaaat
     2341 gagtggaagg tgagagatag aacgatgaaa accacgaaaa gacaacatgc attagaaccg
     2401 attaaattag atgcttataa gccgctgcgc gaagtcgtca gcgaaaccct gcgccaggcc
     2461 atcaaggacg gcgtactcaa accgggggaa cggctcatgg aaatccagct ggccgatgaa
     2521 ctcggcgtca gccggacgcc gatccgcgaa gccatccgca aattggaact ggaaggcttc
     2581 gtcgtcatgg ttccccggcg cgggacgtat gtcgccgata tttccttgaa ggatatcgcc
     2641 caggtcttcg agatccgcag cgccctggaa gaactggcag ccggcctggc agctgaacgg
     2701 atcacgccgg acgaactgga atacctggaa cggatcctcg tcgaaatcaa cgaatatatc
     2761 gacaacgacg aattcgataa gatcgtcgat gccgacgtcc gtttccatga cgtcctctat
     2821 catgccnnnn nnnnnnctca ataatctgcg cgaacagatg ctgcgcttcc gctccatttc
     2881 catgcactat cccggccgcc tggcagcgac gtgggaagaa catcggcaga tggtggaaaa
     2941 cattgccgat cacaacagtg ccatggcccg caaagtcgcc aagaagcaca tggaaaattc
     3001 cgaacggacc ctgctcaagg gcatcagcaa agacgaagag tcggcccggc gcgttcatga
     3061 attatttccg cacggcggag aataaggaaa ggaagcgacc atcggatggg acattttctc
     3121 ttgaaccatt tccgccatgt ccacgagaag tcggaacata tggatgactt tgaaaacttt
     3181 ttgtatatcc tgacctacat cttgttcgtc tgtctgttta tccgctccct ggtcgccttg
     3241 tacatgggac gctggaactt gttctaaaac tatatttata ttaaagaggg gtatttttta
     3301 tgatgaagac tttttctctt ccctttggga agggtacaca gaccgtttcg ctcgatgaag
     3361 cacacgtatt gtatgacctg cacggcaatc ccgtcgatgc cgtagccgat gaacaggaag
     3421 ccatccgaca ggctctgcgc caccctatcg gcagcgcacc gctcaaagac gtcgtccagg
     3481 ccggcgatac ggtagctatc atcgtcagtg acattacccg tctggtccat acggcacaga
     3541 tgctgccggt catcgtcgat gaattgaacc aggccggcgt ccgggacgaa cagattaccg
     3601 tcgtcacggc ccaggggacg caccgggccc ataggccgga agaagacgtc atcgtctgcg
     3661 gcgaagacat ggtcaagcgc gtcaaagtcg tcagccatga ctgccgcgat gcgtcgcagc
     3721 tgaccaaaat cgggacgacg acgcatggca atgacgtcta tctcgactcg cacgtcgtcc
     3781 aggccgataa agtcatcttg accggcgccg tatccttcca tcccatggcc ggttttggcg
     3841 gcggccgcaa agccgtcctg cccggcgttg ccagctatga aacgatcatg cgcaaccacg
     3901 ccatggcctt gaccgatacc tttggcggcg gctgcaatcc ccgatgcgaa acgagtctgt
     3961 tggaagacaa tcccctccac gacgatatga aacaggccgc agccctgctc aatccctgtt
     4021 ttctggtcaa caccgtcttt tctgcagacg gcgatctcta tgaagtcgtc ggcggccatt
     4081 ggtacgatgc ctggaaaaaa ggctgtgacg acttgctgca catcgccagc gttcccatcc
     4141 aggaactggc ggatattacc atcgcatcgg ccggcggcta tcccaaggac atgaacctct
     4201 accagagcat gaaggcgccc atgaacgccg tcttcgccac caagccgggc ggcacgatga
     4261 tcctgaccct ggactgcccg gatatcaaag aaccggccat ctttacggac tggttcttcc
     4321 gcagcgacat ggaagccttt gaaagggact tgcgggcaga cttttccatt cccgccttcg
     4381 tcgccttcaa gtcgcactgc attttccgct ccatgaaggc ggtatacgtc gtcacgcggc
     4441 cggaaaactt cgacatcatc cgtcacagtg gcctcatccc ggcagcaaca ctggaagaag
     4501 cctgggaaaa agcgcaggcc gatctgccgg aagactacaa agtcactgtc atgggccacg
     4561 ccgccgccac tttccccgtt cggcagtagg ggcttgtcct gttcggcacg cttgtgggac
     4621 gatcgtgttc gtggttcgta gcatataccc aaatgctcat acccacgatt cacagcgggt
     4681 cgccttttag gcgaccccta cggaaaaagg cactgtcttc atgtgacagt gccttttatg
     4741 ctctacggtt atacgtttta ccatatttat tacgtttgtg catgtattaa aatgactaaa
     4801 ctgtatatct tttttttaca atttccaaga caaatggcac cttgtgtact ataatattca
     4861 taattcacga gaaatagttg caataagtga aatgatggtt ccatttttca ttaaaaagta
     4921 aaggggttct tacaatgaaa gaatataatc cggttacagc tgaagtcatc gacgctttga
     4981 aagccgtcgt cggtgataaa tatgtaaaga ccgatgccga tgtcctggac atctataaat
     5041 ccgatgaaag cctggcaccg tccttctgga agacaccgga agtcgtcgtc ctgccggcta
     5101 atacccagga agtttcggaa atcatgaaga tcgccaaccg cttcgacgtc ccggtcacgc
     5161 cgcgcagtgc cggcaccagc gtcagctgcg gcgccgttcc ggcttataaa ggcatcgtcc
     5221 tgctcatgga acggatgaac cacatcatcg aattgaacga agatggcatg tatatgaccg
     5281 tcgaagccgg tgtccgcact atcgacatcc agaatgcagc ccgcaaaaag ggcctgctct
     5341 acgccggtga cccgtgcagc tccgacagct gcctcatcgg cggcaacttg gctaccaacg
     5401 ccggcggcaa caaagccgtc cgctatggca cgacgcgcca ccaggtctac tccattgaag
     5461 ccgtcctgcc gacgggcaag atcgtcaacc tcggcgctac tttaaagaaa tgctcaaccg
     5521 gcttctgcct ggaccagctt atcatcggtt ccgaaggaac cttgggcatc atcaccaagg
     5581 caacgttgaa gctcgttccc ttgccgccgt ataaactcga cgtcctggcc gtcttcacgg
     5641 acctggccaa ggcgacggct ctggtaccga aactgattaa agccggcctc aacccgacat
     5701 ctgtcgaatt catggataac aacttcgtcc gcagcgccag cgattacagc gacttgaaac
     5761 tgcctcacta cgaagatggc tgttatgata tcatttccat cgaaaccttc aatgaagacg
     5821 aattggacga taaaatggaa aaattggccg ctgtctgtga agaatgtggg gctacggaca
     5881 tcgtcgaagc cgacgaccgc gtctggaaag tccgccgcaa ctgcctggaa agcacgcgcg
     5941 tcttctcgaa agtcgccacg tcggaagact tggtcgttcc ggtcacgaaa atcgccgact
     6001 gcatccagac cctggtcaag gaaagcaaga actacgattt ccgcgtcttc tgcctggccc
     6061 acgccggtga cggcaacctc catttccaga tcctcaaatg cgatatgagc gacgaagact
     6121 gggaaaaaca gctgaaggaa ttccggacca aagcgtataa atacgtctac ggcttgggcg
     6181 gccgcctgtc cggtgaacac ggcatcggct tgaagcgcct gaaatacatg gaagaatata
     6241 cggatccggc cgaattggac ctcatgaaga ccatcaaacg cgccgtcgac ccgaaatgga
     6301 tcctcaaccc ggggaaagtc atcgaagaaa actaaataag tttaagttgt aggggcgccc
     6361 acgtggggnn nnnnnnnnnn natcatgcga cagaacgaaa ggctcccctg ataggggagc
     6421 tggccgccga aggcggtctg agaggtttac ttaaacgtat tactttaaac tataaagaaa
     6481 cagcttaaaa ttataaatta actattggac aatggcagcc tttttatgtt atactagtag
     6541 tacataaaac gataattagt atcaaaatat aaaaaggaga tgttatgatg aacgccattg
     6601 ttgtgtattc ttctcagacc ggacgtattg aacaaattgc acatgccatc gccgcaggcc
     6661 ttccgcaggg gacgccctgc gtcagtgtcg acgacatgcc cgatgacttt tcatcgtatg
     6721 actgcgtctt tgccggtttt tggatcgacg agaaccaggc ggaccctaaa gggcaggaag
     6781 cgttgaagaa aatcggcaac gaccacgtcg ccgtctttgc cacgctctac gacgatccct
     6841 attcggatca ggcctccaag cacctgcgca gtgccgtaga gctcctcgag cctgacagcg
     6901 gcgtcatagg gacgtacgtc gcctggacgg acaaggacaa gtacatgcat cggccggaag
     6961 cgggggaaga cctgcacctg gaccgggcca aggcctttgc ccaggataca ctgagccgcc
     7021 tcaaaggcca gtaagctgca gaaaaggcgc tgtcgcatga agacagcgcc ttttttatgc
     7081 tgtaggggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnccct actacagacg gcggatgctc gttacgaggg
     7201 cgtcccgcct gatagcagct actttcgttc gagaatctct tttttataag cttctgcgcc
     7261 ttgttccggg ctgatatagc cttgttctgc catcaaggtc aggactgttt tctgccgggc
     7321 tttggcggcg tcgtaatttt tcaggggatt atagtaggtc ggcgcctgta ccaggccggc
     7381 cagcatggat gcctgggcca ggtccagttt ttccggcgtc gtcgtgaaat acgtccgggc
     7441 cgcgtcgtcg atgcccgtgg cgttggcgcc gaaataggct gtattcaaat agatttccag
     7501 gatttcatct ttgctgtagt aatgttccag caagaaggcc atgacgaatt cctgggcctt
     7561 gcgggccatg gtccggtcct gggaaaggaa gacattcttg acgacctgct ggctgatggt
     7621 gctgccgcct tcgacggtct caccggattc gatgttgacc aggacggccc ggatgatgcc
     7681 gatcgggtcg atggcaccgt ggtcgtagaa gcggcggtct tccgtggcga cgacggcatg
     7741 ctgcatgggc ttggaaatgg cgttgatggc tacgtagacg gggattttct tgatcttgtt
     7801 gtccatagct tcgtggaagt gcaggtacgg atagatccgg atttccagcg gccgcagcgg
     7861 tgccggccag cttccctgcg gttccttttc ttcttggagc ttggccgccg tctcgacggc
     7921 aggcttggcc gttttcggcg cgtcgaagag caggttccag ccggcacaga tggcaaaggc
     7981 cagggcaaag atggcgatgt attttagttt cattgaattc ctcctactaa acacttttat
     8041 tatactatac tttgcagggc gagggaattg acaggcctgt aaaaaaaagt ttgcaaattt
     8101 tttatgcaaa atacttgcat aatatttcaa ctgggtgtat actatcaaac gtagacagaa
     8161 acaaacacaa atcaatcgct ttggttcatc agatagagga ggaaatcatc atgagtaaca
     8221 cgaaaaaaga tattaaaaaa attgtcctgg cttattccgg cggcctcgat acgtcggtca
     8281 tcattccctg gctgaaggaa aactatcctg gcgccgaagt catcgctgcc tgcgccgacg
     8341 tcggccagcc ggacgacttt gaagccattg aaaagaaagc ctatgcttcg ggcgcttcca
     8401 aagtctatgt catggacatc aaaaaagaat tcatggaaca gtacgtttgg ccgacggtca
     8461 aagccggtgc tgtctatgaa ggcaaatacc tcttgggcac gtccttcgct cggcccctta
     8521 tttccaagaa actcgtcgaa gtcgccaaga aagaaggcgc cgatgccatc gctcacggcg
     8581 ctacaggcaa gggcaacgac caggtccgct ttgaattgac cgtcaaagcc ctggctccgg
     8641 aaatgaccct catcgctccc tggcggatgt gggacctcaa atcccgtgaa gacgaactga
     8701 aatacgctga agcccacaac gtccccatcg actatcagtc cgaagaaaat ccctatccct
     8761 acagcatgga ctggaacatc tggcacctga gccatgaagg ggacgacctg gaaaacccgg
     8821 ccaatgcccc gaaagacatg gtctacatgg tcaccaagac gccggaacag gctccggatg
     8881 ctccggaata cgtcaagatc gactttgaac agggcgtccc tgttgccgtc aacggtcaga
     8941 aactgggtgc tgtcgaactc attacggccc tcaatgaaat cggtgcccgc aacggcgtcg
     9001 gcatcgtcga tatcgtcgaa aaccggctgg tcggcatgaa gagccgtggc atctatgaaa
     9061 acccgggcgg tgccatcatc atgtatgctc acagagaatt ggaatacctc tgcctcgacc
     9121 gcgacacgta tcactataaa gaaaatgtgg ccaacaaatt tgccgaactc gtctatgacg
     9181 gcaaatggtt ctcgccgctc cgcgaagccc tgtcggcttt cgtcgatgaa acgcagaaga
     9241 ccgttaccgg ccaggttaca ttgaaactct ataagggcaa catcatcagc gctggttcta
     9301 catcgccgta ctccttgtac aacgaagaat tcgtcacctt cggcgaagac gacgtatatg
     9361 accagaccga tgccgaaggc ttcattaacc tcttcggcct gccgctcaag atgcgggccc
     9421 tcatgaaaca gcacgtagaa aaacagggta aataaggagc gattcgtatg aagttatggg
     9481 gcggccgttt taccaaggcc acggataaaa gtgtagaaga attcaatgcg tccatccagt
     9541 tcgattgccg catgtatgct gaagatatct gcggcagcat tgcccatgcg acgatgctgg
     9601 ccaaacaggg catcatctcc caggaagaca aagacgccat cgtcaagggc ttgaaggcta
     9661 ttttccagca gatcgaagac gggaacttcg atttctccgt cgagctggaa gacatccaca
     9721 tgaacatcga aaaacgactg accgatgcca tcggcgaagc cggcaagcgc ctgcataccg
     9781 gccgcagccg caacgatcag gtcgccttgg atacgcacat gtatgtccgc cgcgaaatcg
     9841 ccgccgtcgc caagctcctg gtcaagctcc aggaatcgct ggtcaaggct gcagaaaagt
     9901 acagcgacgt catcatgccg ggctataccc acctccagcg ggcccagccg atcctcttca
     9961 gccaccacct catggcctat ttctccatgc tgtcccgtga cttcatccac ttgaaatacg
    10021 cctgggacat ggccgatatc atgcccctcg gtgccggtgc cctggctggc acgacgtatc
    10081 ccctcgacca gcctttcgtc gccaagaccc tgcatttcgg caagatttac gacaacagtc
    10141 tcgatgccgt cagcgaccgg gactacatca tcgccttcct ggaattcgca gccgtcctga
    10201 tgatgcacct cagccgcttg tcggaagaat tcatcctctg gtccagttcg gaattcaagt
    10261 tcatcgaact cgacgattcc cactgcacgg gctcgtccat catgccccag aagaagaatc
    10321 ccgacatctg cgaactcgtc cgcggcaaga cgggccggtt ctacggccac ctcatgggcg
    10381 tcctgaccat gatgaagggc atccccatgg cctacgacaa ggacatgcag gaagataaag
    10441 aaggtgtctt cgatgccatc gataatttga aattcgccct gaccatctat gcggccatga
    10501 tcgacaagat gaccgtcaat ggcaaacata tgcgggaagt gctggaaagc gacttttcca
    10561 atgcgacgga tatggccgac tacctggcca agaaaggcct tcctttccgg gaagcccatg
    10621 ccgtcgtcgg caaggccgtc cactactgca tcgaacaggg caaggtatta cagcagttat
    10681 cgctgcagga attgcaggcc atgagcccgc tcttcgcaga agacatccat cactacttgg
    10741 atatcgaaac ctgcgtcaag cagcgtaaca cgtataacgg cacgtcgcct cagtccgtgg
    10801 cccgccagtg taaagccggc aaagccgcca tggatgggga acaggcccag attgaaacct
    10861 ggaacgacgt cgtcgcctct gtctatgaac tattgaagta aaaagcgatt gaacgaaaag
    10921 gagctgtccc ctggggcggc tctttttcat ttgcaaggaa ttaggtgggc cgcgaaccac
    10981 ggaccactag gccgcaggcc gaaaccacga acaacaccct tgtcatacac cctataaata
    11041 aggtataata aaaaagtatg aaaaaaatta tcttactcgt aacaggaaag gaggggcctg
    11101 cctatgtcaa tagtagtaca aacgacgctg gctcatgacg tttcatataa cggcatcggc
    11161 ctgcatgctg cgaatgaagt tcatatggta ttgcgcccgg ctccggctga tacaggcatc
    11221 gtctttatcc ggacggattt gcccggcaga ccgtctgtac aagcccatct cacgaatgta
    11281 accaatacaa tgcgggcgac gacgttggaa aacggggaag ccaaggtctt cacagtagaa
    11341 catctgctgt ccgccttgag cggcatggaa gtggataact gcttcgtcga aatggattcg
    11401 gtcgaaccgc ccgtcgccga cggaagcagc ctgacttttg tcaagctcat cgaagaagcg
    11461 ggaatccgtg acctggacgc accgcgccgc ctgctggccg tccaaaaaca gcatctcgtc
    11521 caggaggacg ataaattcat cgccatcgtg ccctatgatg gcttccgcat caccttcact
    11581 tcgatcaacc cgcaccccat gctgggcgtg cagttcctgg acacgacgat taccaaggaa
    11641 tcgtatgtcc gcgacatttc ctttgcccgc accatcgcct ttacggaaga attggaaatg
    11701 ctcaaggaat taggcctcgg caaagggggt accatcgaaa acgtcgtcgt ctacgataag
    11761 acgtcgtgcc tgtccgttcc ccgctgtgac gacgaactgg tccgtcataa ggtcctcgat
    11821 atcatcggcg acctgtccct gctcggccgt cctgtccgag ggcacatcat cgccgtcaaa
    11881 tcgagccata aactgaacaa cctgctgacc cataaaatat ttgaagaaat gcagtgattt
    11941 aaggaggaat tctcatgact ttggaagtaa ctgatattat ggaaattttg ccgcatcgct
    12001 atcccatgct gctcgtagac cgcatcatcg aacttgaacc gatgaaatat gccatcggca
    12061 tcaagaatgc gaccttcgat gaacaatttt tccagggcca tttcccggga aaaccggtca
    12121 tgcccggcgt cctcatcgcc gaagccatgg cccaggtcgg cggcgtagcc ctgctctatc
    12181 cggaagaaaa ccgcggcctc atcccgttct ttacaggcat cgacaaactg cgcttccgcc
    12241 atcccgtcat ccctggcgac cagctggtca tgcgggctga catcgaaaag atcaaaggcc
    12301 gcatgggcaa ggtcaaggcc cagtgtcacg tcggcgacac actctgtgcc gaaggggaat
    12361 acctctttgc cctcatgccg ggtcagaaca cgaaaaaata agttaagaaa aatttaaaaa
    12421 atctttactg catgttaaat gacggcctaa atgggcgtat ttgcgagtat acagaggaat
    12481 atagcgggaa aaggcctgat tttgcccggc aaaaccggac tggtattctg cgagtgtata
    12541 taatatgtga tttaggttga aaataagcgc ctgccgctca ggcagggaag cgaggagtga
    12601 ctttaatgac cgaggaaacg aataacatgc ctcagagcat gatacatccg acggctatta
    12661 tagacccgca ggcaaaaagc ggtgccggtg tcaaaatcgg gccgtatgcc gttatcgggc
    12721 cccacgttac cattggcgat ggtacggaaa tcatggccca cgttaccatc gacggctgga
    12781 ccacgatcgg taaggactgc cggtttttcc cgagctgttc catcggttcg gaaccacagg
    12841 atttgaaata tcacggtgaa aagagctacc tgatcatcgg cgaccgctcg gtgttccggg
    12901 aattcgtcac cgtcagtacg gccaccggcg aaggcgaaga aacgcgcatc ggcaacgact
    12961 gcctcttcca ggcctgcact catgtagctc ataactgcgt cgtcggcaat cacgtcatca
    13021 tgagcaactg cgccggcctg gccggccacg tcaccgtcga agaccgcgtc gtcatcggcg
    13081 gcatcgccgg cgtccatcag ttcgttaaga tcggccgcaa cagcatgatc ggcggcctgg
    13141 caaaagtcgt ccaggatatt ccgcccttcg tcatcgccga cggtcagccg gcccgctgca
    13201 tcgggctgaa cagtgtcggc ctttcacggg ccgggattcc ggaagaagta cgccgggaac
    13261 tgaagaaggc tttccgcatc ctgtaccgtt ccggcctgaa cctgcgccag gccattgccg
    13321 aaatggaaca ggaactggac agctatgaag aagtagaaca tttcctccgc ttcctccgca
    13381 atgccgaccg cggtatctgc agagggacga aagattaggc agccgctatt ggctaaaaag
    13441 gggtgtcgcg tctgcgatgg cccctttttt tgctgacagg gggcctgttt ttttgttata
    13501 atataaagat gaatatttct ctttatgaat aaggatgatt gctatggaaa aagtaggatt
    13561 gcttgccggt gtcggcaaac tgcctgtaga atttatgcgc gctgccaaac agctgggtca
    13621 cgaagtcgtc gtcatcgccg tcgtcagtga tgtcgaaccg gaactggctg ccgaagcaga
    13681 tgcttattat gaaatcaacg tcgccaaact ggataaggtc atcaagaccc tcaagaagaa
    13741 tggcgtcgcc aaagtgacca tgattggcaa ggtcactaag gaaatcctct tcaaaggctt
    13801 gaagttccct gactggcggg ctatcaagct gctggcgaaa ctgcgcaacc gcaaggacga
    13861 tacgatcatg ctggccatcg tcgaagaact ggccaaagat ggcctggaag tcgtcgacca
    13921 gacactgtac ctgaaacccc tcatgccgtc ttgcggtgtc ttgtcgcgcc gtaaaccgac
    13981 ggcgtcggaa gaagacgata tccgcttcgg tttcgtcacg gccaaggcca tgggcggcat
    14041 ggacatcggc cagactgtcg tcgtcaagca caaggccgtc atggctgtcg aagccatcga
    14101 agggaccgac gcttgcatcc gccgcggcgg cgccctggga cgggaaaaat ccgtcgtcgt
    14161 caaagtggcc aagcccaacc aggacccgcg tttcgacgtg cctgccgtgg gcaagcagac
    14221 catcttgtcc atgattgaaa gccattgcaa tgtcctggcc atggaagccg gccggacgct
    14281 cttcgtcgaa caggacgaag tcctggacct ggccaaccgc cacgatatct gtatctgtgc
    14341 tgtcgataag aatttttagg agagcctatg aaaatcatgt tttctgccgg tgaggcgtcg
    14401 ggcgacctgc acggggccaa tttggcccgg tccctgcgcg aactggatcc gcagatcgaa
    14461 ctgctcggta tgggcggcaa ggaaatggca gcggccggcg tcgacatcgt ctatgacatc
    14521 aagaacttag gcgtcatcgg cgtcggggaa atcatccgca agattccctt tttcttccgc
    14581 ctccgcgact tcctcgtcga tacgatgcgt aaagaaaagc ccgatgtcct ggtctgcatc
    14641 gactatccgg gcttcaacat gcgcctcatc aaggtggcca aagcggcggg catccccgtc
    14701 gtctattata tcctgccgac catctgggcc tggcacaaga gccgcggcaa gaccattgcc
    14761 aagtatacgg atctggccat ttccctcttt ccctttgaag ccaagatgta tgaagacatg
    14821 gggacgaatg tcatctatgc cggccatccc ctggtcgata cggtccatgc gaccatgagc
    14881 cgccgcgagg cctgtgctca tttcggactg gacgaacgga agaagacggt cctcctcatg
    14941 ccgggcagcc gggtccagga agtacgcggc ctcctgccgt gtatgctgga agcggccaag
    15001 ctcatccgca aagaggcagg ggacgtccag ttcatcctgc ccaaggcgtc gactatcgac
    15061 gaaggcctcc tcgatgagat catccgggct tcaggcgtcg ccgtcacggt cggtgaagaa
    15121 aaagtatacg atatgatgaa tctcagtacg gcagccatcg cggcttcggg gacggcgacg
    15181 ctggaaacgg ccctcatggg cctgccgacg ctcctggtgt accgcgtcaa ccgcctgact
    15241 tactggttat cgaaagtcct ggtccatatc gacagcatcg gcctgccgaa catcatcatg
    15301 ggccatcggg tcatccctga attgtggcag gatgaggtga cgccggaaaa catcgctgcc
    15361 gccatggtgc ctatgatcac cgataaagtg caccgtgaag cattgcacaa atccctggcg
    15421 gccgtccgcc gcacgatggg ggaacccggc gctgtccagc ggacggccca ggctatctta
    15481 gattttgcaa gagaaagagg tgcccatgaa gcaatacaat agttggcaaa gttataagag
    15541 attattaatg ttcatcaagc cgtacaagat gcgcctcgtc gtcgccgtca tctgtatggc
    15601 cctgtccggg gcgagcgacg tcgtcgtccc gtggctcatc aaggacgtca tcgataaggt
    15661 cctggccaat aaggatatct acacgctgaa cctcatcgtc atcggtatcc tggtcctctt
    15721 cctgctccgc gggttcttct atttcggcca gaaatacctc atgagtttcg ttggccagaa
    15781 aatcgtcaac gatatccgcg aaaccctgta tcgtcacttg cagaccctgt ccctgtcgta
    15841 cttcgacaag cacaagacgg gcaatatcat gagcaacctg accaatgacg tcacggcttt
    15901 gcagacggcc attgcaggca acctcatttc cttcgtccag gaagcggtca tcctcgtcgg
    15961 ctccctgggg tccatgctct tcctctactg gaagctgacc ctgttgacct tggtcatcgt
    16021 ccccctcgtc gtctttacga tcaacttctt cggctcccgc ctgcgccggg ccggccacga
    16081 cgtccagggc aagatggctg acattacgtc gcttttggaa gaggctattt cggggatccg
    16141 catcatccgc tccttcaacc gggaggactt tgaaatccgc cgcttcatgg tccagaacga
    16201 ccgcaacttc tgggctctca tgacgacgac caaactgacg gccctgctga cgccgttcat
    16261 ccagttcttc gcagccatcg ctgtcacggg catcatctgg tacggcggca tgagcgtcat
    16321 cgacggcgaa atgacggccg gcgccctcat cgccttcctc atctactcca tcaacctggc
    16381 caacccggtc cgccgaatca gtgaaatcta cggcgatatc cagaaatccc tggccgctgc
    16441 cgaccgcgtc tttgaaacaa tcgacaccga accggatgtc aaggaaaagc cggacgccat
    16501 cgt
//
LOCUS       DAWABZ010000029        16292 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_962_length_16292_cov_3.602882, whole genome shotgun sequence.
ACCESSION   DAWABZ010000029 DAWABZ010000000
VERSION     DAWABZ010000029.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 16292)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16292)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16292
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_962_length_16292_cov_3.602882"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    15334..15343
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3592 a         4928 c         4130 g         3632 t
ORIGIN      
        1 agaggctgtc gcatgaagac agcctctttt tgagttttaa gtttgtagtt tttagcgaat
       61 ggttttaaat ttaaagccat cttctacaaa ctacaaactc cgcactacaa actaaaatta
      121 aaagaaccac gcatgccggc caacgccttc acgcggcaaa aaatcaatgg aaaatggcgc
      181 aggccgtctt gaggaagggc aggcgtttgc tcaggcccgt ccgcgaccgc tggatccatt
      241 tgaggtcata cggttcaaag acgacattgc ggtaggcttc gatgtccaag ggcgtcccgt
      301 cccaatcgga atggatggcc ccggtctgct tgatgagaat atcatagagg atatcgtatt
      361 ccttatcgtc ccgttcatcc cgttcgatga ccgtactgta cggcagggtc ttatgatagg
      421 tcagttccat gcctttgtaa tcgaaataga aaccgcagcg catacggcag ccgtcgaggc
      481 ccgtataatc ggcgattttc ttcaggtcat gaaggatatg gtcatggcct tcttcatata
      541 aattatccgg cgtcgtttcc gtatagtcga aacgaaactg gattgatgcc ccatcaatct
      601 tgcggaaccg ttccagatac ggtttgagct ggtctgccgg atatttcttg tacaatacgc
      661 agttgatgcg gaacggaaca gccaggcggc tcaggaggcc atcattcgat tcctgtacat
      721 agtgctgcat atggcgcgat acgttgatgc aggtgatctt atgacggttg cgttccgtaa
      781 aagcgacgat gtcgtcttct gactgattct caaagacggg cagcgtcgta ttgatatata
      841 ccttgtgtgt cacaggaata cagtccagca tgcgctgcag cgattccata ttggcaaaag
      901 gttcaccgcc agtgaatacg aaatcacaga acggcgtcaa gctgtccatc aggcggatgc
      961 tctggcagat ggcttcttca gaaaaacccg tcatatcttc atattccgcc ttgttgatgc
     1021 agaacgggca atggttgtga caattatagg gcacgaaaac cgtgacggta gcgccgccgt
     1081 tccgtgtctt atacgggtgg accacacgcg gtacagtcgt tgcggccatg ctgattgttg
     1141 ttttcattct tttttcgcct cgaatcgtgg ataagatccc aagctgttcc cacacagcag
     1201 taagatggaa aggaaagtat tttttcacct cacagcagac aatcttgtga cccgaatgta
     1261 gtatccacgc ttattgtacc ataaaagtcc atttctgcaa atgcatgttg cgtattccca
     1321 ttttgcataa tttttatacc aaacaattca tcataaacat tattttattt ttctaaagaa
     1381 atccttacat ttcctttgaa tctaaagccc ggctaatgtc ggccgccgtc tttttgacag
     1441 cttcgatggc caggtcccgt ccttccgccg ttagccggtc tgccggcccg gatacactga
     1501 tagccgccgt aatgattcca tgatggaaaa tcggcgcggc aatgcatgcc atgccgagtt
     1561 cgacttcttc ccgttccacg gcataacccc ggcagcggat cttctccagt tcctggagca
     1621 ggtcggtcca gtcggtcacg gtatattccg taaacttctg cagcgtgcag cccgtataag
     1681 tgtgtaggta ttccggtgaa aaagccaaga ggcacttgcc cgaggccgag caatacgccg
     1741 gacggacggc accgataggc ggtgccgtat caatcgtcct gggcgacttg atcttctcta
     1801 aaatgatgtg ctgcggatag gaagccccat tatgggcaaa ggtcgtaata tggacccctt
     1861 cattgcactg cagggacagg gccttggcat acggataggc gactttaggg aaggcatcct
     1921 tttcccggaa gagcatcccc agggcataga cctggagccc cagccagtat ttccctgttt
     1981 cactgtcttt ctggacaaag ccgcggcgct ccagcgtatc catagtgcgg ctgacagtac
     2041 tcttatgaag gtccatcagc ttgctgatac gggtcacact gaggggcctt tcttccttta
     2101 aaaaaagctg taaaacggcc agggcccggt caatcgattc aatcatcctc gttcccccga
     2161 tccggcatcg tcacgacgcc aagaaattaa tatactttat tatatggaat acaggcataa
     2221 tgtcaagagg gaaacgccag gccccagccg gagagtgcta gtggctgacc gcttcgcttt
     2281 cttcctggga cgtctgatga tggtcgtaca ggcactgcga cagcttgcgg atcaaatcgt
     2341 cacattccaa attgccgcgc cagctcttgg ggatagcctg gaccccatcc tgggtaccca
     2401 tgatattgcc gcagatggcg ccgcacgtat cgctatcgcc atcctggttg caggccatga
     2461 gaactgcgtc tttcagatgg cccgtcttga ggacgcagta tacggccagg gccagggctt
     2521 catcggcttt cttgcccagg cccagcttct tcatggagcg cactggattg cggtcggtga
     2581 cggcttcgtc gacagcccgc aggatagtct tcatgagcgg tgtcccttcc ggatcagcct
     2641 ggaggatgcg cagggcccct cccagggcag cgctgatatc cttaccagct gccagggagg
     2701 caatcatggc actcagcgtg gccgtcgata aataggcttc cggactaccg taggtcaggc
     2761 tggcagcctg gcagccgacg gcaaaggctt tttccgggtc gccggcataa aacaggccga
     2821 taggcgcagc ccgcatgacc gtactgctgc cacaactgtg gttcagcggt tcctgacggg
     2881 tcaatttttt ccccgacgcc agcgacgtca ggcaggtctt gcccggagag cggcgggcaa
     2941 agagggcctc ttcccccatg atgtcgtagt cgaaatcgac ttcatgaggc tggcgcttca
     3001 tccatttttc ctgttccgga tggatgatgc gttcggtctg cgtataatac cagcgcatat
     3061 acgcccggta caggccatct gaaggttcca atccgtcgtg gtcggcccag agcatgccgt
     3121 cagctgtgaa caacgccagc tgcgtattgt ctgagataag gctcttattg ccatttttag
     3181 ccgattttaa aacagtgcgc aatccataag ggccgtattt cttatgaatc gtcttcaaat
     3241 cgtcgaattc aatgacaaac cccaaagcgt cgcctacggc gccgccaaac atgcaaccca
     3301 taaaccgatc tagtccatcc atgtagcatc ctcctatcct cgtctgtcgt ccgagtcttt
     3361 ttttctcaaa aaactttaca atttcgttca agtagtatat aataaaaagc ttatacgttc
     3421 attatacagg ttatgatagg aatttgcaaa taaaaaaagg gcttgtcgca cgaagatttt
     3481 tgccatcatg caaggcccct tttactttat agtttgtagt acggagtttg tagtttgtag
     3541 aagattactt taaatctttt accatttgct gcaaactaaa aactacaaac ttaaagctca
     3601 aaaagaggct gtcttaatgc gacagcctct tttaagttgt tagtggttag cagttagcca
     3661 cgagccacga gtcacgagtc acgagccacc aacattttaa ctcatttccc cgctgcgagg
     3721 aggtcggttc tgccttctac gcggtcgagg gctttcttta cgatggcggc caggccctgc
     3781 atcttgattt ccgggatgga gcagacgccg aggcggacgc cctgggccag gggaccggca
     3841 aagaccaggt cggcttccaa ggcctgacag acggcagccg attgctgcgt cggaatggag
     3901 atgaagaagc cgcctttata gggcacatac ggcaggccgc agcggtcggc ttcataggtg
     3961 aagatgtcgg cacggcggcg caggctcttg gcaaaactca tgcgttccaa ttcgagctgg
     4021 gccatgaggt cgcggtcgct gcggatcttg gtcaggagcg tcatggccgc gcggttgatg
     4081 ttcgaccagg ccgtgcgggc cgaatatttg cccagattgg cgaactcttc gataacggct
     4141 ttgctcgacg acaagccgac gagagcgccg gcgcgctggc catacatagt atagcctttg
     4201 gacatgctga aggcgaacat ggtaaagatg tgttccggaa ggccggcgaa ttgtctcatg
     4261 aacgagcgga cgcggtcctt ttccccggca taggcgatat aggcgatatc gacgaggata
     4321 ctgatgcgct tgccattctt ttcatggagg cggcagactt cgaggacatc ggcccattcg
     4381 gcatcggaca gggcaaagcc cgtcgggttg ttggccggcg tgttgaggat gaggagcagc
     4441 gaatcctgac ggcgcaggat gttggaaaca gcagccgaga acgccgccgt attgaaatgg
     4501 ccctggtcgt ccatcatcga gaaggtgtgc aggtcgcggc cgatttcctg gcacaggcag
     4561 tcataaatgt tccagcgcca gtccgtcgtc agtacggcat cgccgacttc tgaatagttg
     4621 gcaatggcga tatggatggc accggtacca ccggacgtag ccacagcatc gacatagcca
     4681 tcgggacgac agccttcaaa ggtttcgtcg atgacggcct ggcgatagcc ttccaggccc
     4741 agcggcggtg cataggtaat gaaatcgctc ataggcagag agcggtataa ttgttctacc
     4801 acaggcaggc aagccagttt ttcatcgtcg ccggcataac agccgacggt cgcattgacg
     4861 accttatccg ccccgtactg cgctgccgct tcccgggctg ccgctccagc ggcgaatacc
     4921 acgtcacgta atgtcttacc ttctctgctt gctgctgcca taatcgtcat cgtaaaaaac
     4981 ccctctctgc ataatagttc gtgtttagcg gacacttgtt ctctccaaat gtcctgacgt
     5041 cctacattat agcactgtga aaagagtttt tatatagcga aaagcctaaa atactgtata
     5101 cacagcatga atattgtata caatattcat ttgtggtctt cgctgtcgtg tctggcaccg
     5161 gcatcgacgt ggaacgtcgc ctgtttgatg cccttcatgg ccaggagctg gctgacgatt
     5221 tccgtttcat ccatgtaatg agccgccgtc agttccaggt cgatgtcggc caagggtgca
     5281 tcgtcatcat tatgggaatg gaaggtcttg atatgcatgt ggtggtcatg gataaaagtc
     5341 gaaagtttcc gggcctggcc gatgttgttc ttcatgacca ggtgcatggc atagcgcttg
     5401 cggaagtgcc cttccaggcg ggcatcgagg cggacgaaga cggtcaggac caggaaaatc
     5461 agcgccgtcg ccacacaggc tatgacgaga tagccggcac cgacgacgag gccgatggcc
     5521 gatacggccc ataagccggc agccgtcgtc aggccgcgga cgctgcggtc tttctggttg
     5581 gccaggatcg taccggcccc gatgaagccg acgccggata cgacctgagc tgcaatccgt
     5641 tccgggtcgg agttattata gccaaaggtg aagtaataat ccatggcaat attgatagat
     5701 atgatcatgc acaggcagga gccgacacag accagggtat gtgtcctgag gcctgccgac
     5761 ttgctctggg cctgtcgttc ataaccgatg agtccgccca ggatgagggc cataatcagg
     5821 cggatgatgc tgtcttccgt agaaatcata gaggctcaac ttctttcaaa ataattttta
     5881 catccgttaa gaaaacgtta taatggtatt ataatatata gatgcagcag agaacaagca
     5941 aaatgttctc cccgcatcgg agaaagaagg tcatgatcat gaaccgcttc atttgctttg
     6001 ataatctgcg cagtgccgcc gtcgtattga cgctggccgc tttattgcta caatcctgga
     6061 cagcctttta cgaagagagt gccgtcgtcg ccatttccat cgtcttcttc atcagtgcct
     6121 acttcggagc ttcggccctg cgcatccgca cgatatcgtt cttcttgaaa gataaatgga
     6181 aacgtctggg ctggccgggc ctcgtcttag tcctcttcgg catcgaagaa cagctggtca
     6241 catcagccct gccggtcacg tcgtgggtcc cgctctggct cttcctgctc tttgccggct
     6301 tgtccctgat caagcatgtc aaccataagg tcttgcagca ccgcatcgcc tcgaagccat
     6361 cagttccttt cctgctgggc ttcctcgtct tgctggcagc cgccctcttc ctctgtcagg
     6421 ccttcctgcc tcagtccctg gccatccctt acatcgcccg gatccgcccg gactggctgg
     6481 tcctgtccct ctttgccttt gccctagggg tctacacctt ccgccatcgc tggttcacgc
     6541 cgtctggcta tcagcctaaa agcagcagcc tcttcggctt cgccatcatg gtcggcgtct
     6601 atgccgcctg gatgcccggc aagggagcct ctatcctgac agcgctgacg ccggctttac
     6661 tgctcgtcgc cgccgtcctt ggcctgacgg cctggttcca tcagcagttc gataagcact
     6721 cggcctggtc ccacacgctg caccgcattt cctacggcct gttcttcgtc agcgaaccga
     6781 tcatcctcca ttcgatgtgg ttcttacagc ccctggccgt cgccgtatgg ctcaaggtcg
     6841 tcattacggc cctggtcctg gccatctacg gctatctgct ctgccgttac gccctgttcc
     6901 acatcccatg ctttaaaagc aagggataat agctggcgga cactagcccc ggcaggccat
     6961 ctgtgctatg atagatagca agtttgtttc tttacattat cgataaggag tgtgcctact
     7021 tggatacatt attacaactc atggaatcgt acggctacat cgcgatgttc gtggcaatgg
     7081 cattggaaaa cgccaatatc cccatcccca gtgaagtcgt actcggattt gcaggcttcc
     7141 tcatttccca gcaaatcttt tcattctgga cgacctttgc cgtagcctgc gtcgccggcc
     7201 tcgtcggctc ggtcatcagt tactggctcg gctcttatgg cggccggccg ctcttgttaa
     7261 agtatggcaa atacatcttc ttcaacgaac gtaaattccg catggccgaa gacttgttca
     7321 acaagtacgg cggcattgcc gtcatcatct gccgctgcct gcccggcgtc cgcacgttca
     7381 tttccttccc ggccggcgtc gcccgctatc ctttctggaa attcgccatc ttcaccgtca
     7441 tcggcaccat cccctggacg ctgctcctcg tatgggccgg ctccctcctg ggcagtcact
     7501 ggcgggacct catacagtac aaccacatct tcctcatggc cgtcatcgcc gtctgtgtcg
     7561 tcgccaccgt cgtattcgta tggcatagac gccgccggag aaatgcatca tgaaacaatg
     7621 gttcttaaca catcgttacg gcctgaccgt cgccttagtg gcagcaatga tcttgctgcc
     7681 atttttaggt gctgccccgc tcatggaccc ggacgaaccg gtctacggcg aaacggcgcg
     7741 ggaaatgctc ctggctgggg attggctgtc accgcgcatc ttcggccagt actggtacga
     7801 taaaccaccg ctcttctact ggctggaaat gctgtcgtac agctttttcg gcgtctctga
     7861 catcgcatcc cgcctgccca gcgtcatcat ggccatggca acgacgatgt acgtctacgt
     7921 ccaggggaag gaattattca ataaacccat cgccttcctg tcggccctgt tcctggtgac
     7981 cagtgccggc ttcatgtata tcggcaaggc tgccgtcacg gacatgaccc tcatgttcac
     8041 cctgaccgtg accatggttt ccttttaccg taaaaactat tatatagcct atatcttctg
     8101 cggcctgtcg ctcctggcca aaggccctgt cggctacgcg ttcccggcgc tcatcatgct
     8161 catttatctg gcctggacgc ggcagtggcc gctcttgaag catatgaaga ttccccaggg
     8221 catcctgctg gccttcctgg tcggtctccc ctggtatatc ctcatgtacc gggtccacgg
     8281 cgaagtcttc ctggacacct tcatcggcta tcacaacatc acccgcttcg cggcgccgga
     8341 acatccggga cagaacagtc tcttcttctt catccccatc ctcttcgccg ccatgatgcc
     8401 ctggacggcg gccctgttcc cgacaatccg gcggctgttc cacaagcggg accccttccg
     8461 cgacatactc cggttctgct tcgtctgggc ggcctttacc ttcgtcttct tctccctctc
     8521 caagacccag ctggtcacgt atatcgcccc gatgttcccg ccgacggcat atctgttggg
     8581 ctggtactgg taccgctgct atatcgagag gaaattttcg cggcccttgc tggtatcctt
     8641 ctatatcctg ggcctgctgc tgctcgccag caatgccatc ccgctccacg aaaacgccgc
     8701 ctttttccga ccggccatcg cctggggttc gggcctgctg gcagcggcat tattcctccc
     8761 ggcctggtgt ctgtggcgcc gccgctaccg gtcggctttc gtggcaggca tcgtgatgat
     8821 gatctgtttc tcctgggcgg ctttcggctt catcatcccc cagttcaaag gccacgtcac
     8881 atcttacgag acagcccaaa tcctgccgga tgtctatgac ggccattccc cactctatat
     8941 cgaaaaattc ctgcgcccgg gcatcgccta ttacagcggc atgtacggaa cggaatgggg
     9001 ccccgtcaag gacccggacc tggaagaatt gctcgcagag aaggataaga ttttcctggt
     9061 catgtcgaaa tcgaccttcc ataagctggc caaatcccag caattgattg accgcttcgg
     9121 catcgccgcc gaaacgccga cacaagtcat cctcatcaac catccttagg aggtgtcccc
     9181 atgaaacagc gcacattcct gaccatcatc ttcgtggcag cattcctttt cctggccgtc
     9241 ggcatatctc agctggccgt cacggacccg gtcgaatcga attacgccct gacggcactg
     9301 gaaatggtcc gcagcggcga ctggctgtcc ccgcagattt acggcacgta ctggtatgat
     9361 aagcccattt tcctgtactg gctcctgagc ctgtcttaca gcctcctggg gacgacggat
     9421 ctggcagccc gcctgccagc tgtcctcttc ggcaccgctt cctgcaccct ggctgcctgg
     9481 ttcgccctgc gccagacggg ccgccgcacg acagccatcc tcttcgccgc catgaccgtg
     9541 acatccctga ttttctggtt cgtcagccgc tccgtcatca ctgaccagcc cttgtatttc
     9601 ttcagtggcg ctaccttgtt tttcgcctat atcggcctga cagaagggaa gaaaggctat
     9661 atgacagggg cttacgtcat ggctgccggt gccgtcctga ccaagggccc ggtcggcctg
     9721 gtcctgcccg gcctgttcct gcttctcttt accgccatcc agcgccggcc cgattacctt
     9781 aaaaggctgt tcccgccggc aggcatcgtg ctcttcctgc tcctctgcct gatctggtat
     9841 ggccccatgt acagccgcca tggcatggat ttcatcgatg gcctgctggg cttcaacaac
     9901 gtcgtccggg cgaccgtatc ggaacatccc gaatacgacg tctggtatta ctacctcgtc
     9961 ctcgtcccaa tcagtctcct gccctggacc ggcccctgcc tctacggcct gtggcggcgg
    10021 cgcagtcatc acgatgagta cgtattcatg gccatctggg ccctcgggac aatcctgttc
    10081 tacagcctca tggcaacgaa atacccaacc tatgcctata tcgccaactg gcctctctta
    10141 tacctgggcg cccgcacgat gcaggcctgg ctcgacgagg acgcccgcta tgcctggctg
    10201 gcagccctca tcccggcctc cgtctacagc ctgctcttcg ccgtattggt ggcctttgcg
    10261 gataaagcgc ctttccccgt ccaaggcctg ttgctgctga cggtattcct ggccatcatg
    10321 gctatcctga cctggctggc gtggtggcag aaagcctatc tggccctgcc cttcttcctg
    10381 attacgacga cagtcatcac ctacctgatc gtgaccttcc aggtcctggt ccccttttat
    10441 caataccggt ccgcccaatc cctgaccgtc ctgaacgatg cggccccggt ctatttcttt
    10501 gaagagtacc gcacgtccta tccttattat acgggacaga cggcatactg gacggcaccg
    10561 gccgactacg acgaaaatgc ccggctccgg cgcgatgccg tatgggctaa gaaacacgtc
    10621 tacccgacag cttccgaaga ccaggtgtta tccagcctga atcagcacca gaacctgacc
    10681 ctcgtcgtcc cggaagggaa atataaagat tataaagcct cggaattttt cagcctgacc
    10741 cggaaagtag gccggatcgg cccgtattat atcttcaagc ccagccctta ggataggagg
    10801 attttccatg gaagaactca tcgacaaact ctgtgcctac catcatcccg gcgtattcaa
    10861 tccctggtcc gaatatgacg tctcctgcga catcagtccg gacgcgccag ccatccgctg
    10921 ccagcagctg cgggaatatt tggcgccccg cctgacccgg gcccgcatcg tcctcatcgc
    10981 cgaagccgcc ggataccagg gctgccgctt cacaggcatc cccattacct gcgaacgcat
    11041 gctcctggac gaacacaaac agatacatgc tgacgtcatc ctgggccacc gcggccagcg
    11101 cacgagccgc cccgacagcg ccttcatgac cagccgtcct cagcgggaaa aaggcatgaa
    11161 cgagccgaca gatacctacg tctggggagc catcgtcgac aacggcctgg acccgcgcga
    11221 cgtcttatta tggaatatct tccccttcca tccccacaag acctcgccct tctccaaccg
    11281 cacgccgacg gacagtgagc tggccgacgg cctcgtgtac gccaaagccc tgctgagcta
    11341 ctgccggccg gatatacgcc tggccgccat cggcaggaag tcagcagaaa ccttgcagaa
    11401 tgccggcttc ccggccatcg ccatgcgcca cccggccaac ggcggagccg gtctttttcg
    11461 ggaacagttc acacgttttt ctcgataatt cctttaaaaa atcggtaaaa ttcgattttt
    11521 acattaaaat tgtcggcgtt tcagcaaaaa cattcagaaa aatcattgca tcgacttcac
    11581 aaattgttta taatcatagg tacaccacag tatatacgag ccttctctct catacagtga
    11641 cggctcgttt ttgtataccc aaagggggat tacgtatgaa taaagaaatg cgcgatctgc
    11701 cgcgcccgca aggcctgtac gatccgtctt atgaacacga tgcctgcggc atcggtatcg
    11761 ttgccaatat aaaggggaaa aaatcatacg gcatcatcga cgatgccctg acgatcctgg
    11821 aaaacctccg tcaccgcggt gccgaagggg ctgacgccaa cagtggcgac ggcgccggca
    11881 tcctggtcca gattccacac cagttcttca agcgcgaatg tgaagtcctg ggcttttccc
    11941 ttcctgacga aggtgaatac ggcgtcggca tgatttttgc ccatcgttat gaaacattcc
    12001 gtcataaaca gatggaagcc tttgaagaca tcgtccgctc cgaaggcctg tccatcctcg
    12061 gctggcgcga tgtccctgtc gatgaaagcc tgatcggcaa cattgccaaa cagacgtgtc
    12121 ctcatttcct gcaggtcttc atccgcaagg acccgaccat gaagacccag atggactttg
    12181 aacggaaatt atacatcatc cgcaagctga cagaaaaagc catcgtcccg gaaagccaga
    12241 aaaacggcac ggacttctac atcgccagcc tgtcatcgaa gaccatcgtc tataaaggca
    12301 tgctgacgtc catgcagctg cgccacttct tcctcgacct gtcggacctg gatttcgtca
    12361 cgtccatggc cctcatccat tcccgtttca gtaccaatac cttccccagc tgggcccggg
    12421 cccatccgaa ccgttacatc gtccacaacg gggaaatcaa caccatccag ggcaacatca
    12481 actggctcaa cgcccgtgaa ggcaaatcga aatcggactt cttcccggac atggaaaaag
    12541 tcttccccgt cgtcgacgat acgggcagcg actcgtccat gttcgacaac tgcctggaat
    12601 acctgtacat gacaggccat tccctgcccc atgccatgat gatgatgatt ccggaaccgt
    12661 gggaacggga tccgctcatg agcgacgaaa agcgcgattt ctaccgctat cacaacttca
    12721 tgctcgaacc gtgggacggc ccggctgcca tcggcttctg cgacggcacg gtcatcggcg
    12781 gcatgctcga ccgcaacggc ctgcgtccgg cccgttacta tgtcacccgc gacgaccggg
    12841 tcatcgtaag ttctgaagtc ggcgtcgtca acatcccgcc ggaagacgtc ctctacaagg
    12901 gccgcctgga accgggcaag atgctcctcg tcgatacgca gaaacagcgc atcatcgacg
    12961 acgacgaaat caagcacgaa atcgcgacgg aacaccctta cgacgaatgg tataaagaac
    13021 acatcgtcaa cctcgacgac ctcatgacgg gtcagaacat cgccaacagc gatgccatca
    13081 tcccttatga cctgaaggaa caggaaaaag tcttcggcta tacccaggaa gatatggaca
    13141 aggtcatcct gcccatggcc cgcgatgccc agcacgccat cgactccatg ggcgtcgacg
    13201 tccccctggc cgtcctcaac gacaaacctc agctcctcta cgattacttc aaggaaaatt
    13261 tcgcccaagt caccaacccg gctatcgacg gtgtccgcga aaagacggtc atgtcgtcag
    13321 ctgtcatggc cggcaacgtc gccaacatca tggaaccgaa cgaagcgtcg acggcagcct
    13381 tgtacctcaa aggcccgatt ctgaccaacg aacagatgtc ggtcatcaag tccctcatga
    13441 cgccgaaact gcgggcggct acgctgtcca tgctctatcc cgtcaacagc ggctctgaag
    13501 gcatggaaac ggccatcgaa tcgctctgca tcgatgcctt gaaggccatc aagaacggcg
    13561 ccaacatcct cgtcctgtcc gaccgcggcg tcaactcgcg catggccgcc attccggccc
    13621 tcctggcctc ggcagccctg caccattatc tcatcgacaa gggcgtccgc tccgacgtcg
    13681 gcctcatcct ggaatcgggc gaaccgcgcg aagtccatca tttctgtacc ctcatcggct
    13741 acggcattac ggccatcaac ccgtacatcg ccctggaatc catcaaggaa ctcattgcca
    13801 acaagaagct ccagaaaatc gattacgaaa cggccaaaca gaactacatc gacgccgccg
    13861 tcaaaggcat cctggccgtc atgtccaaga tgggcatttc gacagtccac agctaccacg
    13921 gcgcacagat ttttgaagcc gtcggcatca aacaggacct gatcgacaaa tatttctgca
    13981 acacgccgtc ccgcatcgaa ggcctgggca tcgctgaaat cgccaaggaa aacgtcctgc
    14041 gccaccaaga agcgtacagc acgggagata aactggaacg gggcgacttc taccagtacc
    14101 acaagggcgg ccagccgcac atcatcgacc cggaaacggt ccagctcctg cagaaagccg
    14161 tccagaccgg cgactacgca acgtataaaa aatacgccga ccatgtcaac cgtcagacca
    14221 ttttccgcct gcgcgacctc ctggactttg aatatcctgc cggcagcagc atccctatcg
    14281 aagaagtcga acctgtcgat tccatcgtca agcgcttccg cacgggtgcc atgagttacg
    14341 gcgccctcag caaggaagcc catgaatgtg tcgctacggc catgaaccgc ctgggcggca
    14401 tgagcaatac cggcgaaggc ggcgaagact cggcccgttt cgatacggaa acgaacgacc
    14461 gcatcaaaca ggtcgcatcg gcccgtttcg gcgtcaccag cgactacctc atccacgccg
    14521 atgaattgca gatcaagtgc tcccaagggg ctaaaccggg cgaaggcggc catctgccgg
    14581 gcaataaagt ctatccggat atcgccaaga cccgtcatgc tacgacaggc gtcgccctca
    14641 tctcgcctcc gccgcatcac gatatttact ccatcgaaga cctggccgaa ctcatctttg
    14701 acctcaagaa tgccaaccgc aaggctacga ttggcgtcaa actgacgtcc ggagccggca
    14761 tcggcacgat tgccgccggt gtcgtcaaag ccaaggccga cgatatcgtt gtcagcggtt
    14821 acgacggcgg gaccggcgct tcgccgcgga cgagcctgcg ccatgccggc ctgccctggg
    14881 aaattggcct gtccgaagtc cagcagacgc tgctcctcaa ccgcctgcgc gaccgcgtca
    14941 ccctggaagt cgacggcaaa ctgctcacag gccgcgacgt cgccattgcc gccctcttcg
    15001 gcgctgaagt cttcggcttc ggtacggcac cgctcctggc catcggctgc gcgtctgcca
    15061 cctcaacacc tgcccgtacg gcgtctgcac gcaggatgaa aaactgcgca aacgcttcaa
    15121 gggcaaaccg gaatatatca tcaacttcat gcgcttcgtc gcacaggacc tgcgggaaat
    15181 catggcccgc ctgggcttcc ataccatcga cgaaatggtc ggccgctatg actgcctgaa
    15241 acagcgcgat cacttcggca actggaaagc caagacggtc aacttgaaga acctcctgta
    15301 ccgtccgtac acggatgcgt cggtcggcca gcannnnnnn nnngcatttc acgacgcccc
    15361 agaaccacaa gatcaatacg accttggata tgtccaaact gatccgcatg tgccggccgg
    15421 ccctggaaga acagaaacat atccgggccc gcctgcgcat caagaatacc gaccgcgtca
    15481 ccgggaccat cctgggcagt gaaatcacca agcgctacgg cgaaaaaggc ctggccgaag
    15541 acacgatcat cttgtccttc gtcggctctg ccggccagag cttcggcgcc ttcatccctt
    15601 gtggcctgac gctgaccctg gaaggcgatg ccaacgacta cctgggcaaa ggcctgtcgg
    15661 gcggcaagat catcgtatcg ccgccgcggg aatcggtatt ccctgccgaa gataacatca
    15721 tcatcggcaa cgtcgccttc tacggtgcca ccagcggcga agcctacatc aacggcacag
    15781 ccggtgaacg cttctgcgtc cgcaacagcg gcatcagtgc cgtcgtcgaa ggcgtcggca
    15841 accacggctg cgaatacatg accggcggcc gggtcctcat cctcggccag acagggcgca
    15901 actttgcagc cggcatgtcc ggcggcatcg cctacgtcta cgacttggac ccgaataaat
    15961 gcaacaccga cctggtcaaa ctggaaccgc tcaccgacga cgacgaaaaa gcggctgtca
    16021 aagccatgct ggaaaaacac gtccactaca cggacagcaa tctgggccac atcctcttgg
    16081 aaaactggga cgacaccgtc actcacctga ccaaagtcat tcctgaagcc tatgaagaaa
    16141 tggtcgccct catcgcccag gcagaagccg aaggccatac cgaccaagaa gcacacatga
    16201 ttgcttttga aaagaaacac ggaaagaacg gaaagaattg aacctctccc gcttatagaa
    16261 gcgggagatt cttgagaagg ttgctgttta ag
//
LOCUS       DAWABZ010000030        16174 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_966_length_16174_cov_3.092127, whole genome shotgun sequence.
ACCESSION   DAWABZ010000030 DAWABZ010000000
VERSION     DAWABZ010000030.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 16174)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16174)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16174
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_966_length_16174_cov_3.092127"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3533 a         4300 c         4483 g         3858 t
ORIGIN      
        1 gcgtgaacca ggtatttata atagtatact cgaggctatc gccaatggag ccaataggat
       61 ttccaccatc tccgaccgca ttcacgaaga ccggagcaaa tgcacgaaat atctgcaagt
      121 tttacagaat atccggctgg taaagaaaat catcccctgt ggccagccta agacgagcaa
      181 aaagggaatt tacgtgattg cagaccatta ctaccgtttt tggtaccgct acacgttctc
      241 acaaaaaaat tactactttc tcttaggcga agaaaaagcc gcccaagaaa tcatggacgg
      301 catatctgac tatatgggac ctgtctttga agaaatctgc accgaatacc tctggcgcca
      361 ggccaaggct cacgccctcc ccttcattcc cgctgtcatc ggcaaatggt ggggcactaa
      421 tcccctattg aaacaacaag acgatatcga tatcctcgca ctggacaaaa cagaaacaga
      481 gggaatcttc tgcgaatgta aataccggaa ccgcccgatg cccatggaag agtatgatga
      541 cttagtccag gctacggccg ctttcccgaa agtaacgaaa aagcacctgg tctttttcag
      601 caaaggcgga tacacccaac ctgtcataga acgcgctcaa agagaaggca cacacttgct
      661 aggtataagc gatttgttct aagcgccgta tttatgtaaa taatcggcat agtaaaattc
      721 atcggtcgtc atgttgaagt agcggatgcc ttcgcgcagg cccaggaagc ctgggatgag
      781 ggtccagcag aagagggcgt agatggcccc gcggacaggg cgcttcaaat agaagtaatg
      841 gatgccgaag gatcccagga agaaggccag gacggctgta atcaggcgct cttcgttgat
      901 tttatggcgc agttccggtg taatcgacga gaaggccccg tccgtatagg catcgaagcg
      961 gtcggcggct ttctgcttcc actgagccag ggagcctttc agggacgacg gcttctttct
     1021 ccgcgcggca tccgccgggt cgtacaggcc ttgggcagac aggttctggg ccatgaccgt
     1081 cgtttctccg tcgatggtca ctaattccgc ttccatgacc tgcgtgaact gttcttcata
     1141 ggcctggtcg agttggctga gcaggttctt gagcttgttc tgcgtgttga ccactttgtc
     1201 cgtcttaacg cctgttacgg ccagtttctt atatttatgg accatgtaga cggcccggtc
     1261 ctgatagtag tcgatgaagt gttctgccag tccgattcgt tcgggatact gctgtaaata
     1321 gtagagaaag ttacgggcaa tgtgctgcat attggcaacg gtccggcgca ggtccgggtc
     1381 ggtcagcccc aatcgttccg cttccatgcg gttgaagtcg ctgacagcct tttcaaaggc
     1441 cgcctggtca cggctggctc ccttcccttt gctgccgggc cacttcaaag aaagcttgtg
     1501 cggctgccgt tccaggcgat acaggatata cagcgacagg ataacgccga tgacaccgac
     1561 aggatggtta taaaagaacc aggcaccgcc gccgatgagc aggcagaaaa aagaaaggac
     1621 atataaaata tatttcatcg aatttctcct ccatctcatg cccttattgt aacaaatgtg
     1681 gggagatata gcaaaaggac ctgccgcaaa agcgacaggt ccttttctgt aggggcgacc
     1741 acgtggggcg cccgctccgg gatatgtacg atttcccggc caatctgcta gaaaaatcat
     1801 ccaaatccat aacgggtcgc ccacgtggcg acccctacga gccactagcc actagccact
     1861 agccactagc cactagccac taactatttt acaacagcat tgttgatgat agccaggaag
     1921 tcgtcggctt tgaggctggc accgcctacg aggccgccgt cgatgtcggc agagcctgtg
     1981 agttcagcga cgttgccgcc tttgacgctg ccgccgtaga ggatggaaac gtcttctgcc
     2041 gtagctgcgt catagagggc agccagtttc ttgcgaatga tagcgcagac ttcttctgcc
     2101 tgggcagcgc tggccgtctt gcccgtgccg atagcccaga ttggttcata agcgatgatg
     2161 actttcttga cgtctgctgc cgtcaggcct tccaaggctt tttcgatctg gctttcgatc
     2221 caggactgca tgatgccctg ttcgcgctgt tccagcgttt cgccgcagca aacaatcggc
     2281 gtcagattat gagccagtgc ggctttggtc ttcttgttga ccgtttcgtc cgtttcattg
     2341 aagtacgtcc ggcgttcgga atggcccagg atgacatggg tgacaccgat gtcgacgagc
     2401 atagccggcg aaacttcgcc agtgaaagcg cctttttctt cccagtgcat gttctgggct
     2461 gcgacgtgga tcggcgtacc tttggtcatt tcgacagcca tgggcaggta cgggaagacc
     2521 ggagcgatga tgacttccgg ttttacgtcc tgcgggactt tggcgacgag gccctggata
     2581 gtttctttgg cgtcggcacc gagatggttc attttccagt tgccggcaat gatggtttta
     2641 cgcataatga gtttcctcct ttaaaggtga atgattttat ttatcgctca gggatacgac
     2701 acccgggagc tgtttgcctt cgaggtattc cagggaagcg ccgccgcccg tggagatgtg
     2761 gctgatcttg tcggccaggc cggtcttgtt gacagccgag acggagtcac cgccgccgac
     2821 gatagaaaca gcatcggaat cagcaacagc tttggcgact gcttccgtac ctttagcata
     2881 gttttccatt tcaaatacgc ccataggacc attccaaacg atgagtttca tcggagccag
     2941 ttctttggcg aagagttcct gtgtcttcgt gccgacgtcg aggatcatcc aatcagccgg
     3001 gaccgcatcg acgggaacga ctttgtgatc agcatcgttc ttgaattcag cggcgaccat
     3061 gacatcgacc ggcaggagca gtttcgtacc ctgttcctta gcctggttga tgagctgttt
     3121 agccaggtcg atcttgtcgg cttcgacgag agatttgccg atgccataac cctgggcagc
     3181 caggaaggtg ttggccatgc cgccgccgat aatcatgagg tcgactttcg gcagaaggtt
     3241 ggaaatgact tcgatcttgt cggaaacttt agcgccgccg atgatagctg cgaaggggtg
     3301 agccggatta tcgacagcac cgccgatgaa gcggatttct ttttccagga ggaagccggc
     3361 agccatgggc aggaacttcg taatgccagc gacggaagca tgagcgcggt ggcagcagcc
     3421 gaaagcgtcg ttgatgccga cttcagccag ggaagcgagc tggcgggcga aatcttcgcc
     3481 gttcttttct tcttctttat ggaaacggag gttttccaga agcaggactt cacccggttt
     3541 gagggcatca gccatggctt tcgtttctgc gccgacgcag tccggagcga atttgacgtc
     3601 gatgccgagg agttccgaca ggcgtttagc gaccggtttc agggaatact tttcgacgac
     3661 ctgtcccttc gggcggccga gatggctggc caggatgaca gcagccttgt ggtcgagcaa
     3721 gtatttgatg gtcggcagcg tcttctggat acgcgtgtca tctgtgatgt ggcagttttc
     3781 atcaaggggg acgttgaagt cagcgcggac aaagacgcgt ttgttttcga catttacatc
     3841 ggtgatcgtt tttttcatga aaggttcctc ctccaattaa agcagttgta tgattttttc
     3901 agccgcacct tcatcagtga ccagcgtgcc cgtatggcag gcccgcatga cgccgacgat
     3961 ggcttctgcc ttgctggcgc caccggctac ggcgatgatg tgcggatgat atttgatttc
     4021 ccgtaatgaa atccctacat tattcgtacc atataagatt ttgccgtcca ggccgcaata
     4081 gcagcccagc gattcgccac aggcgccgag gctgtgcagg gcatcgcgct ggagcgccgt
     4141 catgccccgc ttgcgggcca tgcggtcggc ccggccgatg ccgaagatga cgacctgggc
     4201 ccgggcggtc atggcgacat tttcctgggt ctgacggtca gctttgagca tcatctgcag
     4261 cgccgagtcg ctcaggcctt cggggatgtg gatcatgcgg tacgagctgt gagtcttgga
     4321 cgcaatgttg gcggcaatat agttggcctg gacttcgacg tggttcccca tgctgccgcg
     4381 ggccggcagg atggtcacat tggccagaca ttccggcagc atacgggcca cttcggccat
     4441 ggtcgaaccg ccgctgatgg cgacagtatc gacgctgcca aagaggtcgt tgagcaagag
     4501 ggcgccacgg cggcccagtt cttccttcgc tgtcttatcg ctgtcactgt caccgggaac
     4561 gatgtagaca ttcttcaggt ccagcttctg gcgaatctgg cgctgcatgt catccaaatt
     4621 gttcaggcgc aggaaacatt ccaacagatc cggcatgagc cgtttgcctt ccggctccat
     4681 agcaatgccc agcgtcgtaa agcggacgat accgctgcgc cgcaggacgt cgacgtgcga
     4741 ccggacgacg cgttccgaca ggcccgaaag gccggccagg gtccggcggc cgacgggtcc
     4801 ggcgtcggcg atatgccgca gcagcaggta acgttctttg attgtcccca tgacatcggg
     4861 agctattttt tgaaagatac tcaatgtatc ttgttccatc ttactctctc ccttcacgat
     4921 gcgatagaac aacagagtat aatcactgat ccagcaggca tttgccggtc atatcggcag
     4981 gaatggccat accggccagg gtcagcatag tcggggcgac gtcggccagg atgccgtcgt
     5041 gcaggtgttc ccctttatgt tcttcactga ccaggatgca cggtacatga ttggtcgtat
     5101 gagccgtgta agggcatccc gtatcgggat cggccatctg ttccgcattg ccatggtcgg
     5161 cgatgatcag gagctggccc tggacgggct tcaaggcttc gacaatcttg ccgacgcagg
     5221 tatcgaccgt ttcaacggcc ttgacagcgg cttcaaagac gcccgtatgg ccgaccatat
     5281 cggcattggc gaaattgagc atgatcatgt catatttatc ggccttgatc tgttcgatga
     5341 ctttttccgt cacgataggc gcgttcattt ccggctgtaa atcgtaagtc gcaactttcg
     5401 gcgacggcac gaggatgcgg tcttcaccgg gattgggttc ttcttcaccg ccattgaaga
     5461 aatacgtgac gtgagcgtat ttttccgttt cggcaatgcg cagctgggtc ttgccggcct
     5521 tggccaggac ttcgcccagt gtgtcgggga tccgttcttt atcatagacg acgtgtaccg
     5581 gaaggtcgtc ttcgtaacgg gtcatggtcg caaagtacgt gtccagtttc ttcggacgcg
     5641 ggaagccgtc gaaatcgggg acgacgaaag ccgtcgtcag ctgacgggcc cggtcggggc
     5701 ggaagttgaa gaagacgacg ctgtccccat cctggacggg atgtttatgg atgactgtcg
     5761 ggatgacgaa ttcgtcggaa acgtcagctg cgtaggaatg ttccaggcag gtctgggcat
     5821 ccggttcctg cggtccttcg ccgagggcga cggcatcata ggctttttcg acgcgttccc
     5881 agcgcttgtc gcggtccatg gcatagtagc ggccgctgat ggtggcgatt tcaccgacgc
     5941 cgagttcagc gcacttgtct tccagttcct ggaggtattc gccggcactg cggggcagta
     6001 cgtcgcggcc gtcgaggaag gcgtggatgt agactttttt caagccatag cgcttcgcca
     6061 tttccaaaag accatagaga tgctgttcac tgctgtggac gccgccaggc gataccaggc
     6121 ccagcaggtg gagggccgag tctttttcct tgacgttttc cacggcaccg acgagagccg
     6181 gcttcttgaa gaaagctccg tctttgatat ccttggtgat cttggtcagc gactggtaca
     6241 caatacgtcc cgcaccgata ttaagatggc ccacttcaga gttgccaatc tggccgtcag
     6301 gcagaccgac gtcttcgccc gatgcgccga gatacgaatg aggatattct ttccaaagtt
     6361 tcggcaggac cttgaggtct gcctgggcta cggcgttatc tttagcgatg ctgctgcagc
     6421 caaaaccatc catgatgacc agcattacag gtttcttttt cgtcattaca gtatttgact
     6481 cctttgtgat gtgagaaagt aaaaagaggc tgtcccatta agacagcctc ttttcgagtt
     6541 tgaagtttgc agtttgtagt ttgcagtgat tggctaaaaa ttcaagccct ttctacaaac
     6601 tacaaacttt acactaaaaa ccttgaaaga aaaaggagcc gtgcatgaga acgagaacct
     6661 tcatgcgacg gcccctttag ggttcaatct gatttgtaac gggaggctgt ttacccgtcg
     6721 ggaaatgcgt cgctacaaat tagtgtttgc tgttgtagtc gttcatgaca gcaatgaggt
     6781 tgaggacttt gttggagtag ccgatttcat tgtcgtacca ggaaacgact tttacgaagg
     6841 tgtccgtcaa agcgatacca gctttagcgt caaagatgga cgtgagcggg cagccgagga
     6901 agtcagagga aacaacagct tcgtcagtgt agccgaggat gcctttgagt tcgccttcgg
     6961 aagctttttt catagctgcg cagatttcgt cgtacgtagc cggtttagcg aggttgaccg
     7021 tgaggtcgac gacggaaacg tcgagggtcg gtacacgcat ggacataccg gtcaatttgc
     7081 cgttcaattc cggaatgact ttgccgacag ctttagcagc acccgtcgtg gacggaatga
     7141 tgttgccagc agctgcacgg ccgccgcgcc agtctttcat ggacgggccg tcaacggttt
     7201 tctgcgtagc cgtcgtggag tgaaccgtcg tcatgaggcc ttctttgatg ccgaaagcat
     7261 cgttgaggac tttagcgatc ggagcgaggc agttcgtcgt gcaagatgcg ttggaaacga
     7321 ttttctggcc tttgtatgta tcggtgttaa cgccgcaaac gaacatgtct gcctggcggc
     7381 cgtcagcacc ctgtttggaa ggagcggaga gtacaacgta ttttgcaccg gctgcgaggt
     7441 gtttttcagc gaggtccatg gtgaggaaac ggcctgtcga ttcaacgacg tattcagcac
     7501 cgacttcgtc ccatttgagg tttgccggat ctttttcggc cgttacgcgg atttttttgc
     7561 cgttaacgat gagctggctg ttttcgacgt cagcttcgat cgtgccatcg aactggccgt
     7621 gcatggtatc atatttgagc atgtatgcca tgtagtcaac cgggcagagg tcgttgatgc
     7681 ctacgatttc gatgtcatca cgagtctgtg cagcgcggaa aacaaaacga ccaatacgac
     7741 cgaaaccatt aataccaact ttaatcattg tcgatacact ccttttgaaa acgaaaataa
     7801 ctcttttaca taattctaag aacttgaggg ccttactact tacactgtaa gtatgccaca
     7861 tcgggtacga ttttgcaata ccatgggcgc tttatgtccc aatttattgc atccgcaaag
     7921 aagcggcgcc tatgccgaca tattccttga tggccagaca ggagttgaat aagtaaaaag
     7981 actggggcac gatggaatag cccgaccagt tcgtcgctgc actcgtgtgg taatcacagg
     8041 gatagggcgt cacgtccagg ccttggcgcg taaagaagcg gaccgaccgg ggcatatgga
     8101 aggcgctggt gaccaggatg ggcttcttcc agccctggtg ctcgcagatt tgcctggaaa
     8161 aagcggcatt ttctgccgta ttgcggctct tgtcatccat gaaaatctta ttttccggca
     8221 cgcccaggga taagagcatc cgcttttcga tttccgattc attggcgtcg cccttgagga
     8281 tggtcccgcc tgataagagg atgggcagat ccttcgcctt ttgcaggcgc agggccataa
     8341 ggaagcggtt ggccgccgca ctgccgacct gaccggttcc atcgaaatca ggaacgccgg
     8401 cacgagaacc accgccgagg aggatgatga cgtcgccgtc tacatctgcc ccctggggct
     8461 ggtcataggc cgtttccaaa ggatggatga ggccattcga cacgggttcg atagagagca
     8521 gataaaagac agccgtaata gcagccaaag cccagcggcc gggactgtgc ttgcggaaca
     8581 tgtaacacaa caggagaatc aaagccagga cgatgccacc cataggcagg atccaggcat
     8641 aaagccattt aatgatatat aataacaaaa caaagacatc ctttcgtata gttaagcctc
     8701 tcctgctagg agagatggcc caaaagaccg gagaggctgc ttgtaaacca taaatcgccg
     8761 cgacggcaaa ctgcgacctg cgaccggcgg tctgcgaact gcaatctgcg aactgcggtc
     8821 tgcgggccgc ccgctacagc tgtcctttct ggcatccccc cctgcacctc ccgctggtcg
     8881 gtacctggtg ggccagaaaa gctttgggcg gcccctacgc atattatatc ataaacttca
     8941 ttggcaaaag gtaaaatatg tgctatactt attttgaatc cttgtaaagg gatattctgt
     9001 ccatggggat gtaaaggttt cgacgtggat ggttgtatca tgaatagcga gccggggttt
     9061 catctgcccg taaatcggtg aaccttttaa attaaacgca aaagaaaaca attttgcttt
     9121 agctgcctaa tcgcggctaa cgtctcctgc tgctaatcct gcgggcagct cgtagacgtc
     9181 acttagcggg ataccacatg gctctgcctg gcagtcgtgt cgggaaactc acaggctgga
     9241 atcgtctagt ttgtccttgt acgaaaacgg tttaagatcc taacaaggtc tgcgctcgga
     9301 gaagttcatg gggcggcatt tacggacagg agttcgactc tcctcatctc caccatgata
     9361 aaaagggatt gtcgcacatg cgacaatccc ttttaagttg ttagtggcta gtggttaggg
     9421 gcccctgata ggcccttttt ggcccgtcag ggactgaccg gaaagaagga caggacggtt
     9481 gccaaagcgg agagccttgc tggagctgtc agcgcagctg actgagaggt taaacctctc
     9541 cggccctttc gggccacctc tcctatgagg agaggcttgt agataaaaaa tttggaataa
     9601 accattgaca aattgatgca caaggtctat gataagggcc gtaagttaaa catcgatttt
     9661 atattttgac attcgatgac gagaaagagt acatttttac ggaagcgcac agcgagccgg
     9721 gtatggtgaa agccggcggt ggaaggaaaa tggaaaatca tctctgagaa ttgcagttga
     9781 agtgagtaaa ctgcaccgtc tgatccacgt tacaggaaac gcgtatgata gtacgttgaa
     9841 aagtgccttg cttttttttg gcgaggaata ttgggtggta ccgcggatat gaacttccgt
     9901 cccttgtgga cgggagtttt ttttatttca tgtttatctt atgactatat gactcacaat
     9961 aggaggaatt catcatggaa aacatcaatt tcgacagtct gaaacgacgg agagatcttc
    10021 gcatcatttt accggtcttt ctcgtctcga ttatagcctg catcgaccgc gtcaatattt
    10081 cctacgccaa actgaccatg acccagaccc tgtcctggtt atcgccggaa gtatatggtt
    10141 tcggcgccgg catcttcttc gccggctatc tgctcctgga aatccccggt tccctgttcg
    10201 ccgcgaagtt ctcggcatcg aaatggattt cccgcatcat gtttacctgg ggtatcgtca
    10261 gcctgtgcat ggccttcgtc acgacggaat ggcagttcta cctgtgccgc ttcctgttag
    10321 gcgcatcgga agccagtctc tacccggtca tctattccct cttgttcccc cgctggttca
    10381 cggcagctga acgggcccgg gccacgtcgc tcatgttgac atcgcttctc ctttcgacga
    10441 tcatcggcgc accgattgcc ggcgtcctcc tggaacattc cttcttcggc ctcttaggtt
    10501 ggcaggaact gtttatcctg gaatccatcc cggctctttg cttcgctttc tatttcttct
    10561 tcgccgtcaa agaccgtccc gaccaggcca gctggctgac ggatgctgaa aaagaatact
    10621 tgaccaccat gtaccaggaa gaacaggcca agatgcagag cgtcaagaaa tacacagttt
    10681 tccaggcctt tacggatacg aaagtcttga aactctgcct catctacttc ctctgggtcg
    10741 tcggtttctg gggcttctac ttctggatgc ccacggtcct caagaacctc tccggctggt
    10801 ctacgtcgct catcggcggg gctatcgcta tccccatgat ggcagccctc gtcgtccagc
    10861 tggtcatcgg ccatacggct acggttacgg gcgataaagt atggcacgtc ttcggtgccc
    10921 tggccgtcgg cgctgtcggc ctcggcttgt cgcctttcgc tcataacatg ggcgtcgcct
    10981 tgttcctcat ctgcctgtcg gcaatcggca tccacgcttc caggggcgta tggtggacca
    11041 tcccgacgac gttcctttcc ggcccggccg ctgccggctc catcgccctc atcaactcct
    11101 gcggcaacct gggcggcatg ttcggtccga acatgatggc ctgggtccag gctcagacag
    11161 gcagcttcga catgggttac tacctcatgg ccgccttcat gttctgcgcc ggcctcctgg
    11221 tcctgacttt gaaatataaa gtcaacggcg ccgccaaaaa agacgtcctc ctcgacgcca
    11281 aagccaacgt ccagcctaca gtcggacatt agtggctcgt ggctcggacc cacattgtag
    11341 gggcgccacg tggggcgccc gttatgaata actggcgcct acgccaccga gctgtaaggg
    11401 tccaaaaaaa gcacccccta ggatggctta ggggatgctc acataaaccg tgcgggcgcg
    11461 ccacgtgcac gcccctacag ttcgtaaaaa ggcactgtcg cataaagaca gtgccttttt
    11521 acgtgaatca ggcggtccgc cctttgggcg gcccctacaa gccacgaacc actagccact
    11581 aacaccccat cgctttcagc acagctccgg ccaggacggc gtagccttcg gcattgggat
    11641 ggacgccgtc ggcatagaga ttgcggcctg tccgccgttc ggctttgagc agggcttcgt
    11701 agaagtcgat gacgatgcat tccgtttctt ttccgtagga aaggagccat tgccgatact
    11761 ggcggagccc gtcgttgtgt ttttctacgt cactggcatt ttgccagccg tacatggcgc
    11821 tttccatctt ggtcagcggc gggatgccca ggtatacggg cttcccctgc tccttgaaag
    11881 tcgtgacgat agtccgcata ttctggcaga ttttctccaa ctccccgccg aggaggatgt
    11941 cgttggagcc gcccatgatg aagaaggcgt cataccggct gaggtcgaac tgatggaggc
    12001 gatagaccat gcccgtcgtc gtatcgccgc attggccttc attgtcgata tggatatggc
    12061 tttgggccga gaccagggcc gtccagctct ggtcgggccg gacgtcgaag ccgtaggtca
    12121 ggctgtcgcc caggcacagg agtttcacgg gtggacggct ttctggacgg cctttacggc
    12181 agcgtccgtc gacagccacg gggcatcgaa ctggcagccg gctttttcca ggtccatctg
    12241 gcaggcgaag attttcggga tggcactggt ataaatatca tggtcataca tatagcgcag
    12301 cgtcgtttcg acgtcggcat cgctgaggat gtggccgtgg tcgatgagga ccagccggtt
    12361 ggcatagggc agggctgctt ccagcgtatg ttcgatgacg acgatggtat gaccgtattt
    12421 gacattcaag tcgtggatca attcatagaa ttcatgggtc ccttccgggt ccagggccga
    12481 cgtcggttca tcgaagacga ggatatccgg attggtcgcc agtacgtcgg caatgaccag
    12541 gcgctgtcgc tggccgccgg acagcttcgt gacctggcgg ttgcggtacg gcgtcatatt
    12601 gaccttttcc agggcttccg aaatagcctg gccgatctta tccggtgcga cgccctgatt
    12661 ttccaggctg aaggcgattt cttcttcaac cgtcatggcg acgagctggc tgtcataatc
    12721 atcgagcatg gcgccgacac gggttgccag ctgggaaatc gtcgtctgcg tcgtcgcctg
    12781 gccgtcgacg tagaccatgc ctttcatgac gccgccgaaa taatgcggga tagcgccggt
    12841 catagccatg cacagcgagc tcttgccgct gccgctttca ccggtaatga ccaggaagtc
    12901 gcctttattc acggtcatgg tgacatcgtc caggacaggc gtcttctgct tgttatagat
    12961 gtacgacaaa tgttccatgg aaatgaggcc ttcctgggat ggcttcaaat catacatgct
    13021 gtggtccgac gtgacttccc gttcttccag ggaatcgagg atgccccggg atacgaaaag
    13081 gcgcagggcc gggaaataca tgagcggtgt aacgatggca ttcaaggcgc cgatgacgac
    13141 aaccaagggc agcatggccg taaaatagac gacattcggc agattcatga cgaacttcag
    13201 gatcgtgacg aaagcgccgc cactcatgca ggtcgctgca aagccggtca ggacgggcag
    13261 gcacgaatga cctttgaatt tgatgaatgc caggttcttg acgatgaacg tacaggtcag
    13321 ggcgccgagc ggttcactga tgaggttgcc ataaggaaag cccgacttcg acgtcaggac
    13381 gttgaccagg gcagcgacca ggccgatgcc caaggcctgc ttgtaactcg gcttggtcag
    13441 gcagatggcg acacaatacg tggcgatggt ccagttcggc gtgacgccgc cgacgctcgg
    13501 tgagaccagg tgcaggatcg tgccgatggc cagcatgagc gtcgtaatgg tgatccagcg
    13561 gaactggccg ccctgacgat gatgaaattc taatttagca atatctgcaa cagttttcat
    13621 ctttcttccc tcctaaacca agtcttgcag gcgcaagtaa acgacaaaac agctcagggc
    13681 cagcatgatg acgatgccca catagtcgcg gcctttcggg cggacattgc tcatgaagct
    13741 gtgttcgttg ctgccgaaac cgcgtaattc cagactgagg gccatcgttt ccgaccggcc
    13801 cagggacttg agcatcagcg gctggacgac gctcatataa ttgatgactt tcttaaagac
    13861 attgcccttc gtcgacagac cccggcaggc ctgggcttcc tggacggcct tgctttcggc
    13921 gatgaaatcg ggcacgaagc gcagggccgc cgtgaacatg aaagcatatt cataaggtat
    13981 cttgatctgc ttgaccaggg cggccgtcaa atcctgcagg cgcgtcgtcg tcagcaagat
    14041 gatgaagacg atggtcatgg ccagcatgcg caggcccgtg acataagccg attccaggga
    14101 cccgccgccc aggagctgga cgacagccag gaagacggcg aagacggcca ggctgaagac
    14161 ggccttgagg ttgcgcgaca ggctgccgca taatcccaag aggatgagtt cgatgacgac
    14221 taagatgacc aggggcagcg tcgtatgcat gaccagggcc caaaaggaaa aggcgaaggt
    14281 gccgatcaat ttcgtaacag ggaccaaatt cttcatcatt ttttcatacc tccaatccgt
    14341 tccagcatgg ctgtcgtaaa ttctttcatc gatttacaat aaggaaaatc aggaaccgcc
    14401 atggataaag tgacggcatc gggccggctc agacccaggt catataaatc atcgcgctgg
    14461 gtgaacagtt cctgcggcgt cccgtcgaag gccttggtat gattgccgat gaccaggacg
    14521 cgctgacagt attcggcgaa aatatccatg tcatgactga tgatgaggat ggtcatgccc
    14581 tttttattga ggtccgtcag caaggccatg agctggtcct tgtcttcact gtcctggccg
    14641 ctcgtcggtt catcgagaac gatatatttc gtatgcatgg cgatggccga agcaatggcg
    14701 acgcgctgtt tttcgccgcg gttcaagttg ggcggatagg cttcagccag ttccgtcagt
    14761 tccgttgcag ccagggcttc gtcgacggcc tggcggaact tgtcgcagct catgcccagc
    14821 tgctgcggcc cgtaggctac ttcttcacgg accgtcggcc ggaacatctg ccgttccggc
    14881 cgctggaaga cgtagcccat gaaatggctg cgcacagaag catcgtcatc gttgatgacc
    14941 ttgccgtcat acaggatttc cccggatacg ggcttttcca gccccatgag caggcgcgtc
    15001 accgtcgtct tgccgcagcc gttgcggccg ccgagaccga tgaattcgcc ctcaccgatc
    15061 tgtacattta catgttccaa tacgggcacg ccattgccgt agttgaaact gacatctttc
    15121 atttccagca tctttctctc cccttctatt tgaagacaca aaaaaacctt ccgtccctat
    15181 acagggacga aaggtttgat ttcttccgcg gtgccaccca gattgactca gtgagtccag
    15241 ctctacatgt acggcataag cgatacacgt ttctttgata gcggtggaaa tacaccgtcg
    15301 caccctactg gtcaccgttc aggccgcatc tcccgaatcc attctatccg ccggtccgtg
    15361 ccgaactccc actgccatcg gctcgctgga actgccacaa tcggatatac tcgtttcgtt
    15421 catcgacaaa tccatttaga ttttgcttta ttatatactt gtaacgcgcc tattgtcaag
    15481 cctcaggccc gcctattcag ccggttcgat ggtgccgttg tatttatcgg caatgaactt
    15541 cttgacagca tcggaatgga agatttcagc aatcttcttg tacgtcgggt tgtccttgtc
    15601 ctgagcccgg acagccagga ccatgacggc cagcggttcg tctttcggtt ccaggaagat
    15661 gccctgttct ttgacgttga ggccggcact catgacgtag ttcatggtga tgacggcggc
    15721 atcgacgtca ccgaggctgc gcggcagctg ggctgcttcg atttccttga actggaaatg
    15781 cttcggatta tctacgacat cagccggcgt agccttgaac ccgacgccgt ccttgagctt
    15841 caagaggcct gcccgctgga ggagtgccaa accgcggccg ccattggtcg ggtcgttggg
    15901 aatggcgatg acagcgccgt cgggcaaagc atcgacgctc ttatatttgt cgctgtaaat
    15961 ccccatgcgc atgaggatcg tattgccgat aggcactaaa tcagtgccat tctgcttatt
    16021 gaaattatcc aggaacggct tgtgctggta gctgaccaaa tcgatgtcgc catcggccaa
    16081 agccttgtct ggcgtaatat agtcggaaaa ttcgacgact ttaacctgat tctgcaagaa
    16141 gtctcccgcc tctttaggcg gtgagatgaa ttgc
//
LOCUS       DAWABZ010000031        16139 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_970_length_16139_cov_3.827841, whole genome shotgun sequence.
ACCESSION   DAWABZ010000031 DAWABZ010000000
VERSION     DAWABZ010000031.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 16139)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16139)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16139
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_970_length_16139_cov_3.827841"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2768..2825
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3641 a         4144 c         4311 g         3985 t
ORIGIN      
        1 cgcattaaga cagcctcttt ttctgtaggg gcgcccacgt ggggcgcccg cccatgcaaa
       61 tcggctacag gcatcataac atatacctga cgggctcgcc acgtgcgagc ccctacaatc
      121 aaaaaaagaa gcttgtcgca aatgcgacaa gccctttttt tacagtccgc aactcacagc
      181 gtagtccgcg gctgtgtcaa accctctcag gccgccttcg gcggcctgct cccctatcag
      241 gggagctttt ctgcaaacta caaactctgt actacaaact attatttgtt catttcttct
      301 ttcatctttt tcaaacccat gggtttatct tcaaagagga aggccggcat gactttgact
      361 tcgtcgatga tcggcgtgaa gcccatctgg tcgaggacgt ctttctggag gtcgatgccc
      421 ggggcgattt cggtcaggtg gacaccgtct tctttcattt cgaatacggc acgttctgtg
      481 atgtagagga catgctgttt atgtttacgg gcatagttgc cgctgaaggt gatgtgaccg
      541 acttcgggaa cgaatttctt gactttccct tcctgcagga tgtgcagttc accgtctttg
      601 atttcttcgc ggagtttccc tgtcgtaaag gtcccgcaga agacgacgtt cttcgtattc
      661 tgggtgatgt tgacgaagcc gccgcagccg gcaatgcgcg taccgaattt ggatacgttg
      721 atattgcctt ctttatcgca ttcggccagg cccaggcagg tcaggtccag gccgccgcca
      781 tcgtagaagt cgaactgtgt agcctggtcc atgtaggctt cggcattgac agaagccccg
      841 aagcggacgc cgccgagagg tacgccgccg atggccccgg cttcgacagt catggtcaag
      901 ctgtcgccga tgccttcttc gttggctacg gacgagacga gttccggaat accgatgccc
      961 aggttgatga cggcttcact gctcatattg agaagcaaca gggctgcccg gcgggcaatg
     1021 actttcttgg cattgagcgg agccggatcc agggctttga cgggaatcgt cgtttcgccc
     1081 gtcagggacg ggtcatattc gcagtcgaag gactgctgcc gtttcgtttc gtcatcgacc
     1141 tgaacgacgt agtcgacgta gatgcccggt actttgacca gtttcgggtc gagcgtgccg
     1201 cctttgacga ttttttcgac ctggacgacg acgataccgc cgctgttatg gacggcctgt
     1261 gccagcggcg tggcatcgaa aggcgatact tccttggtca ggacgacgtt gccccattca
     1321 tcggcataag tcccgcggat gaaaccgata ttcatatcca tgcgcggata gaagagctgg
     1381 tcatggcctt cgatgttgat gactttgacg aagtcttctt tggtgacgtc attgagacgg
     1441 ccgccgctga tgcgggggtc cgcaaaggta tcgaggccga cgtgggtaat cgtgcctaag
     1501 cgatgagctg cgacgtcacg gaaatactgg gacagcgtgc cctggggcag gttgtaggcc
     1561 tggatcttat tttcattgac taatttctgc agggccgtaa cggcgttcca gtggccgagg
     1621 atggcttttt tcaacatccc ttcgtgggcg aagtggtcgc cgccgcggcc atccgtattc
     1681 ccctgggaag cggcatagat gagcgtcaaa tctctcggcg aacccgtatt gaggaagcgt
     1741 tcttccaggg cgctggtcag cgcttccggc tgtccggaac cgacgaaacc attggtcgtg
     1801 acgacgtcac cgtctttgac gagctgagct gcttcttgag ctgtaattat tttcacttgt
     1861 ttcatgtctt ccatctccta tttcttcacg tttgttatat gcatttcgtg aattcattat
     1921 acaaaggaag catatggaat gatagacgaa aaggatggac taaattggta ggaaaaaatt
     1981 ccatgaagtc aaattactgg gcttccaggg cattccaggc ttcatcagct cgttcgacga
     2041 acatctggcg aaccgcttca ttttctccca ggccatggat ataggtcgta accgtgaagc
     2101 cttctttttc caggatactc ttatgggaat cggcatcggc accggccatg tcgttattgg
     2161 catggtcgcc ggcgaccatc atcatgggca tgaggatgac gtgcttgatg cccttggctt
     2221 tcagctgggg aatgactgta tccagatggg gccagccttc gacagagtag atgtagacat
     2281 tgcccagatc agctgcatcg aggcggttct ggacgacgga gtaataagca ttggccggat
     2341 gcggcgtgcc atgggccatg accaggatgg cgtctttctt gccaattttc gggaactgtg
     2401 tcgagaaggc tttgacgaat tcagcgatgt cgtcgcgctg gtcttcctgt cccatccaat
     2461 acatgagcgg cgtgccgagg gtcaatttct tgaaatcatg tttgtagagg tcgaaaatag
     2521 ccgtatcata agcatattcc atacccggga tgatgtccag ggaggtaatg gccacgcgcg
     2581 tatagccttc ttttttcagc tgggccatgg cctgttccgg cgtcggtacg gtcacgcctt
     2641 ctttagcctt gatgcggtcg acgatgatat gcgacgtgaa ggcgacgacg actttctgat
     2701 ccggatgggc cgccttgata gccgatacag tctggtcgat ggtcttctgg cggctgtcct
     2761 tataggtnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnngatgg cgtctttatt ggcttcgttc tgcatgtccg ggttgttgta cgtgcggacc
     2881 ccgatttccg cggccttttt caaagccggc gtagcgtcct tgacttccgg gttcagcgta
     2941 tagccggcag caaaagaggg cgccgaaaaa gcaccaaacg acgtcagggc caaagataaa
     3001 atcagcgcgg tctttaattt tttgtggaac ataatcatct ctcctttgat aaaaaatata
     3061 aaaaaagagg ctgtcacatg atggcataag ccttcatgcg acagtccctt acatacgagt
     3121 ccttcacggc cagcagctca taaaaagcgg ctcgccgtaa aaatcgccat ccccatcgct
     3181 cgcaggtcaa ccggtgatcc taatcaggca gttcttctgg ctcggattca tggccatggc
     3241 tgcgccttcc catacgcgga cgtacagtga catcattgca gcctggctcc tccttacagc
     3301 ggcgggaccg cgccggtttt gcaccggact tctctattga gcttacgcac ctgattgcac
     3361 tatggaattg tatgaatcct tacaggacta acgtcggggc gacatacgta cgggccagta
     3421 attcctgatt cagggcatag aggcctttgg catcgctgcc gaacatcttg tatttcttga
     3481 tcaaatcgtc ggcattcttt tcttcttcgc cctgttcctt gacgaaccag tcgaagcact
     3541 gcatggtccg gtaatcgtgg actttatcgg cagcctgata aatcttgttg atcagcgacg
     3601 tgacatactg ttcatgagcc aagccagctt tgaggaccgc tttgatgctg tcaaagtcgt
     3661 tctgcggttc ggcgatggcc gagaaggtaa cgcgttcgcc gttgttatgc agatatgtcc
     3721 gcatgagcat cgcgtgatcc cgttcttcct gggcctggac atcataccag ttggcaaagc
     3781 cgtcgaggcc attgtcatag aaatagttgg caaaagctaa atacaaatat tccgaataga
     3841 attcctgatt gatctgggta ttgattaatt cttttacttt tgcatcgagc ataacggtac
     3901 ctccttgaga tatgtctcaa aacgtgtaca gaacttcatc ggttttcttg aaccgtcctt
     3961 attatatcac atttgaccct atcaggaata cttcttattt gactttttgc tcatcaatgg
     4021 cattcttgat gctcttcaag tccttgatat cgacttcttt caggagctgg cggaaccggg
     4081 ccagctgttt ttcgacttcc ggtgacaatt tcgggaattt cgggttaatg gccttgagtt
     4141 ccttgaggac gatctgggcg acgacatagc gcgtatacca cttgtcatca gccgggacga
     4201 tgtaccaggg agcgtacttc gtcgacgtgg ccgataagac ttcgccgtag actttctgat
     4261 agcggttcca atattgccgt tcctcgatgt cagacatgtc gaacttccaa ttcttctgct
     4321 gagtcaggat gcggtcgatg aggcgtttct tctgttcatc tttcgataaa tggaggaaga
     4381 tcttgaccat agggaagcca ttttccgaca agtaccgttc ccagtcgcat atctggcggt
     4441 agcgcgtttc ccagatatcc ttgcgtacca gtttttccgg cataccgtcg cttttgatga
     4501 ggttgtggat gcgggcgacg acgacttctt catagtgcga gcggttgaag atgccgattt
     4561 caccccggcc gggcagggct ttattgatgc gccacatata gtcgtgatcc agttcttccg
     4621 agctcggttt cttgaagctg tgcactttga cgccagccgg attgaggtgg gcaaagacgt
     4681 gtttgaccgt accgtctttc ccggccgcat ccatggcctg caggacgatg atcaggccgt
     4741 agtgattgtc ggcacagagc ttttcctgta aatcctgcat ttcggaaatc gtctggtcca
     4801 gcagttcgcc tttgaccttc ttcttgtcga tgtccacatc gcaggtcgtc gaccactggg
     4861 tcaaatcgat ttttttgcct tcttccgcta aatatcggga aatatccata gtaaaaactc
     4921 ctttctctat ctatagtgct tctcacaaga ctggctgtgg gccgcctttt ccccagcggc
     4981 tccgcttccc gtttttatag ttgatgttga tggaccctat cttgactttg ccattgacaa
     5041 tggaaaaagc gtccgtcgat acgtcaccaa agaacatgga aatgacgacg tcccggaagt
     5101 tcaagatctt gccatcctga gcgaaagagc cggaaatgcc ccggaagggc agctggtgat
     5161 agcgtcccgg gccggactgg aaatcaccgt aaatcgcctt ggtgccggtc gttttatttt
     5221 cttccatgtg cagattcaag tcgacgttgc agcgcaggaa gaggtcatgg gccgcaatgc
     5281 cgcctttgag ctgggagccg gtcatgtccg ccgtatacga tttatcgtcc aggtttacct
     5341 gtccatgacc tttcatggag ccgccgaaga cgccggccgt gacttcgttt atatcgagca
     5401 ggccctgttc ataatggacc tgtcctacga catccgtaaa gaccagggcc gtaatatcca
     5461 gacggtccat cttcaaagtg ccgtcgatga ccggggcata taaagggccg cggatgtcgg
     5521 ccgaaactgt cgccgggtcg tcgatatcca ggccggcccc gagagaacgg ggattgcagt
     5581 cgctcagcaa gagcgacagg ttatactgcg gtgtttcttc tttgaaatcc aggctgccgc
     5641 ggatgcgcag ggacttggca tcgatcatgc cggaaaagtg accggcccgc aagtcgattt
     5701 tcgtcttgcc tttagtatgg atggccgaag agaactcgac atcgccgatc ttgaaataac
     5761 ggcctggcgc ttcagcttcg atgacgctct gatgcagggc gacgtggcag ttaaaggggc
     5821 ggttgctctt aggccctgtg accttgacca aatccttcgg gccgctgctg tgatggtcac
     5881 tggccttctt gatatgctgt aactccattt tatcattaaa gatcaaatga atataggcat
     5941 tattcaaagt cacgtcttcg acagtctgcg taccgatatg accggtcagg acatcgctca
     6001 gtttgaccgt aaactggcct tccggtatct ggaccagcgt atccccatct tcatcgtgcc
     6061 ataccaggcc ctcaaaggac acggttccgc ctggcgtcgc cgtcaggcgt tccaccgtga
     6121 ccgtaccggg aaagagctgg cgttctgcga cgacttggtt gaagatatcc gccgctttgt
     6181 agctcatgta gaaacagagt ccgtaaagaa cggcggcggc gatcaaggta atcgccccca
     6241 tgcgtttcag ttttcgttta tgtcggacat accatttatt ccacatgttc tcccgtcttc
     6301 tttgcactca tatcactgat tttccaggtg ttcccgtctt ttaccatcgt cacctgaccg
     6361 ttgaaatact ggactttgac cttggcactg cctgggatac ggtcccgcgc tttcaaggtg
     6421 aaggaaaagg tcgtcttatc gcttccggaa gcgattttgc ggagattgga aacgtcgctg
     6481 gacaaggtgt tggcgtagcc cggcgcgtaa ccatcatagc cgccgacaga attctggaat
     6541 tccccagtca gcatctggaa ggcatcttgc aggtcgtgat tggaaatggc cttatggtaa
     6601 gccaggaaag cctgcttcgc gccatctgtc ttttcatcgg ccaggcggac gctgatatat
     6661 tcgacggtct ggctgttctt gctgacggca aagcgcagga ttccctgttt cccgtcatcg
     6721 gtcttaaagg tatattcata atgaatctgg gcaccgtatt ccgaggcctg gtaatcgtca
     6781 ccataggcat tgacgacgtc atccaaagag ctgccctgat ggatgccccg cttgctctga
     6841 gctgccggcg aggaggatgt cagcatgatg actttatcgt ctttgacgac gacatccata
     6901 tcggtaaact tatgatgaac ggcaccgtct ttactatccg acgtgctgag ttcccggccc
     6961 aggccgtctt tagcttcatc ccagctttgg tccaggtaaa cgccgtttaa agagagttcc
     7021 gaatcagccg tcttgacaag ctgcgacgat acggtactcc cctgggccgg tagggctttc
     7081 tgttcatggg cgtaccagat gccgccggca atggccacga cgaggacgca ggccagggcg
     7141 atgaagagat tgcgatgctc ctggagctgc gcttttaaag gaacttcttt tttctcccgc
     7201 tgcggcataa ctacaggatg tactgccggc cggctttcag gctgcactac cttttcctcc
     7261 tgctgtacgg ctgtccgatt ggtcagatga ccgtattggg cttgcagaac ctgcagcggg
     7321 gccccgcagg acgggcagaa ttttgccgac gggtcgacgg gctggtgaca ttgcgggcac
     7381 gtatggacgg gtttgccgca atgactgcaa aaggccgcat cagccgctac gggacggccg
     7441 caatgagggc agtatttttc atttactccg tttacatttt gcgttgacat tgcattttca
     7501 tcgttttgat gattggcttg gttatcgcct gtatcgtcaa agttaaatga tgtaaacatg
     7561 ttttcaccgc tcaatgtgcc aatcgacgcc agggaagaaa tgccgaagat gaagaacagg
     7621 gctgtcatgg gaacggtaca catgaggcga aaggcctgat agaaggaagt ccccagccgt
     7681 tcttcccggc gatagatgta atgctggcag aaaacaggaa tcatccagac caggaccagg
     7741 aggacggcat agccgataaa ggtcccatag ccgccgcggg tcatgccgaa catcagctgg
     7801 gtcatactat agacgatatg gccgacgacg gcagccgtac aaagggcgct gaccagccag
     7861 ctgaggatga cgtagtaaat ataacggcgg tattgactga tatagtgcca gcgcttgcga
     7921 ttcacataaa tagtagcgat gatacctaag atacagagaa tcgcagccac agtcccattg
     7981 aaaatttccg caatcaagat caacaatagg tctacgacaa aatagcccag gaacattttg
     8041 agcagataag ccagagcccg cgtcttatag agcaggtaaa tcatgatgac cagaagagcc
     8101 agcgtgacca gcgtattgag gccgctgaaa aaggtcagga catccatggt ccgccgtacc
     8161 ccagcaggac caccgaacaa gccgatcata gccagtcccc gcagggcaat ttcaaataaa
     8221 acgcccagga ccgtcgtcac gacgccaaca tacatcatgg cgataatagc cgtgtacttc
     8281 ttatgctccg tcgggaacca cttgccaaaa tagcctaaag aatccataga aacacctcca
     8341 taaagataag gtaattataa ttttatgtta cacctatttg gcaaaaagga aaaaggcttg
     8401 tcgcatgtgc gacaagcccc ttttcgagta ttaagttttc agtttgtagt ttttagtgaa
     8461 ggcttttaaa tttaaaacca tctgctaacg gctaacagct agccgctgac tgctaaaaaa
     8521 ggcgctgtct tcatgcgaca gtacctttta gtgattaatg gctattatat tttgtcatgt
     8581 ccagtgtccc cagctgttta tcgatcatga tggagttggt gacggaacca gaaacgttga
     8641 ccatggtccg catcatgtcg atgaggggat cgatggccag tatcgggccg atgaggctga
     8701 acgacgaggc catgccgacg ccggacaggc tgactgaagc ggccatggtc gccgtaccgg
     8761 gaatcccggc aatgcccagt gagccgatgg tgacgacgat gacggtcatg accagcatgg
     8821 taatatccag cggcgtcccg gtcagatggc agacgtagac gatgagcata gccgggaaga
     8881 cgccggcaca gccctgcatg ccggctgtcg tgccaaagcc ggcaacaaag ctcgccgttc
     8941 cctgattgac gccgaggcgg gtaaccaggg tttcaatggt gaccggcagg cagccggcgc
     9001 tggaccggga cgtaaaggcc aggatgagca gggccacgct tttcttcaag tagtgaatgg
     9061 gattgacacc atgcagcatg acggccagga tctgaatgat gaactggacg ataagggcga
     9121 catacagggc gatgatgaag atgcccacgt cgaggatgct tgccaggcct ttctgggcaa
     9181 tagtgttagc cagtaaggcg atgaccgccc agggcatgta gtccaagatg agcagggcca
     9241 tgttcatcat ggtcttgtgc aggccatcga tgagtttatg caaaaaggct tccatatcgg
     9301 gcttatctgc tttgatccac cagatggccc ggccaaagac ggcaccgaag atgaccatgc
     9361 cgatgacgtt gttcttgacc atggaatcga cgaggtttcc cggaatgaga gcccgcaggg
     9421 tcgaagccac gctgacgaca tccttcggtt tggccctggc agcttcactg ctgatggcac
     9481 tgcccgaacc gacatcgaaa aggataccga agacgatgcc gatgacggcg gcaatagccg
     9541 tcatgaccaa ggtcaccagc aatgtccgct tgaccaggcg gctcatggtc ttgccagaat
     9601 ccatattgat gacgacgtgg gcaatagaaa tgatgaccag cggaatgacg atcatcttga
     9661 tgaggtcgat atagccattg ccaaagagct ggaaccagtt cgtcgtttcc ttgatgaaga
     9721 cgactttcgt cggatcatca gggaagccag ccatcagctg catgaccagg cccaataaag
     9781 cgccgtccac catgccgacg cagacgacga gcgagaagcc catcttgcac ttatgataga
     9841 gataatgaat aagtccgaag agaacagcca ggacagctaa gaataagact gtccgccaat
     9901 cgctgatcat caggaaggac gtaaaaaatt gactttccat actgaattcc ccctagtaat
     9961 caataaccaa tatacattga ttttcttatt atactaaatt ggtagtcttt tgcaaaggac
    10021 ttttctgctg ttttattgat gaaaaaatag gctgtcgtat taagacagcc tattttttga
    10081 gtgttaagtt tgtagtttgc agtgaatggt tttaaattta aagccatctt ctacagacta
    10141 caaactccgt actacaaacg ataaagagaa agggaccatg catgatggca gaaacattcg
    10201 tgcaacagct cctacgagcc acgagccact aaccctaata ccattcgtta atccagttct
    10261 tttcggtcgg gaagagatcc tgcagggttt gcagagcctt ttcgtttccc tggagttttt
    10321 cgttgaaggc caggtcgttt acatccatga ctccgaaagc cagcagggcc agggtgccga
    10381 tgggcatggt gacgtccggt tgtttatttt cttcttgggt tactttgggt ttaccattgt
    10441 ttacagtcag ggtaaaggtc ccgcagttcc aatctgccag gggatcttct gtatggaagg
    10501 tgacggtccc gtcagctcga taaggcagct gttccagcgc ccctttgacg tcgacgatac
    10561 ggcctgtcat gaagggcatc gtttcatggc cctgtttccc gtcgggatag aagcggtagg
    10621 actggtcatg gatgccttca ttccactgaa aggcgtcgcc ctgggaacgg tggttgtaca
    10681 tgtagcctaa gaggccgcgc ttgcctttgc ggcaggtgta aacgaattca ccgctgatga
    10741 tggtcggttc tcccagctga tagaaggcat agccaatggg ttcatcgcca tcgaagcaga
    10801 cggcgatatt gccttcggcc aactgggctt cgatatggct ccgccagctc gcttcatcgc
    10861 gcagggcata gcctgacagg cctttcgtat aaccttcata aatggcagcc aggtaaggcc
    10921 actggtccgg gctgttgaca aaggcatagc gcacggtccg gtccgtcagc ggccgcaggg
    10981 cttccagatt catcgtctct ttccactgat ggcagtagag ttcataacca tagggctggt
    11041 aaaaaccggc cttgctgggc atgaggatgt ggatataatg accgcggcgc ttcatttctt
    11101 ttagggctgc cgtcagcagt ttcccaccga cgccgctgcg ccgagctgcc gggtgcgtcg
    11161 ccaaaccgac gatatacgac gtgtctacgg ctttgccgcg cagacagagg gtgtacgggt
    11221 tgagatgggt caggcaggcc atatcgtcgc cctcaaagcc catgaggacg ttttcaggct
    11281 gatagaaatg gttgaagtac cactggaaaa aaggatcttc ccgatgttca aaacagtagt
    11341 cccaaaggct ttcgacgagg ccccggtccg attcagccgc taagcggaag tgcatagaaa
    11401 agccccctta tttctgacgc agtgtgacgt cgtatttttc gacgaagtga tccggattgt
    11461 acgattcctt ggaatggcgc aggccgggaa tccccatatc ttcttcacgg ttgatatacg
    11521 tcgtatgcgg ccaggcatgg acgacgaatt catgattgat gacctgatag aggccgcgga
    11581 tcgtcggatc gctcttttca aagtggatga tggccgtatc atcgttgagc atctcaccga
    11641 tagagaaagc gacgatctta ttgtacatgc ggatggcacc gcccgtcggc tggaggactt
    11701 cccagttatt gaagaccgtt tcgatggcat ggcgttcgcc caagagttca tcatcttcgt
    11761 caccggcttc gtgctggtcg taccaggatt tttccgcagc cagacattca tcgaagatgt
    11821 cttccgtaat ggggacgtat tcccagttgc cgaaatactg attgcggaag tgattgagat
    11881 tgtttttctt ctggcggtat ttcttgcccg ataagttgat caggctctgg gtcagataga
    11941 cgtattcata attgtcccgg tccggcgtga agtcataaca gtcttcacag agggccttga
    12001 tacgttcgac agcttctggt gtaacgcctt tcatcaagaa aggatagcca tgttcatcga
    12061 accattcgtg catgcgcagg acgcctttga cgaaactgcc gtctttgcgg gcgaagggcg
    12121 ggatccagaa ctgtttcttg ccgcggcctg agcggatata gaggacattg tcttcttcgg
    12181 cccactggat ttcatagggc ttctgccagg caaagagcgt catgaaggta gcgtcggcct
    12241 gttcatatct tttctgatga aaatagtcat ctataagtgg tttatcttct aagcgaaagt
    12301 ctctgaattc aataatggta atctccccct aaaatattta caccttagat acataaggat
    12361 tgtcttggat ataaaaacgc cagggaaaat atttcccatg tacggcgtaa tcaatatgga
    12421 tacgcggcga aacggccact gtaaacggga cctcttcttc gggatgggaa atatacaggc
    12481 aggaagacgt caaatctaaa gcgttttcct cgcgcgtaat ggccatggcc tgacagagct
    12541 tgcccggtcc attggtcagg ttcctgccgg ctttcctggc cttccgccgt gcggccatga
    12601 ggtctgtccc gcagatgggt tcgatggccc gtatgagcac ggcctggcct tcgccttcgg
    12661 gaccggcgac gacattgaag cagcagtaca tgccgtaaat caagtaaaca tagctgatac
    12721 cgccggcatg gaacatcggt gccgtccgct tggtcaggcc gcggaagctg tgggccccat
    12781 cgtcgggaaa gccgtcataa gaaccgccat aggcttcggt ctcgacgata cggccgctgc
    12841 agatgccctg tggcgtttca tggatgacat actggcccag caggcgtggg gccagcaggc
    12901 tggcatcgac acaaaactcg tcagctctcc atctgcggcc tatcattctt tcttgcggta
    12961 gcctaaggaa tgggaaattt cataggcata ctgactcata gtaaacaaca tcttctggcg
    13021 gatggaatca taaaagcgca tagacggccc ggcaatggtc aacgaggcga tgacctggcc
    13081 ttcaaaattg aagataggca cagacagggc gaacagtcca tgctcgcttt catcctgggt
    13141 aatggcatag ccgttatgac ggatcgtagc aaactcttct tcagaaatat tgccgtattt
    13201 ttcgatatag cgctggcgtt cgtcatcgct cgtccaggcc agcagcaccc ggccggcagc
    13261 acccttatag aggggcagcg attcgccgat gggaatgacc cggcgcaggg aatgggtact
    13321 ttcgacgtgg tccaggcaga tgcggcagtc gccttcggca atgtaaatac tgaccgtttc
    13381 gttaaacaac tgctgtaaac gccgtaaata cggcttggcc agggtgatga cgttttccgt
    13441 ccaatgagac gaatagccca gcgccagggc ccgcggcccc aggcggtact gcttggacgt
    13501 atcgttgcgg gtcagatact ggcgccgttc caatgtgttt acaatgcgga aagccgtcga
    13561 cgtattcaga tgggtatgct cagcgatttc cttcaaggtc atgcccgtat tacattcgac
    13621 gaagaggtcg aggacgtcga ggctcttttc caaggatttc aataaactgc tgtgatgctc
    13681 ttcttttacc aatggctctc acccttcctt ggaattacga tttaactttc atgccgattt
    13741 ccagcgaatc cggcgggtca gcgacgacga tatccccctc gtcgagacca tcgaggatga
    13801 cgacgcggcc gtcttcttca tcgccagtca ggacagaacg ggcatcagcc aggccgctgt
    13861 cattgacaat gtaaacgaag tcaccgctgt cctttgtgcg gactgccgtc ttggggacgg
    13921 taatctggga gacttctttc ggcgtggcaa tgcggacgtg atagaattca ccgggaacga
    13981 acaggctctt ggcgttattg aatgtgacct tggccatata agagccagtg gacggatctt
    14041 tttccgttgc cagataggtc agttcccccg tctcggtttc gcccgaagcg gcgatgatgg
    14101 atacttgcag gctgccgtct tttttggcat cctgtaaagc ctggatatac atttcaggga
    14161 tggcaaaggt agcaaccaaa ggagacatct gctgaatcgt agccaggaca ttgccggcaa
    14221 cggcaacgga cggattatcg gctacagccg tgacccggcc gtcaagaggc gaataaatcg
    14281 tcgtattccc catcatatcg cgggcagcct ggacggcctg ctgcagctgg gcgatctttt
    14341 cgtggtccac agccggagct tcctggacct ggacggtctg cgtcgtttca ccttgagaag
    14401 atccagaaga actgccggaa tcagccatca tctggttgta ttcaactttc gataaggccc
    14461 ccatattata ccaggccttc aattcagcac ggtgaccgct gttgtcgccg ccaccaccgc
    14521 cgccactgct ggcagccggt acggtaacgg tcttgtacgt cggcgtcgat gccgaagaag
    14581 cagctaccag ttccgcttcg gcctgattga cactctgctg atacggtgcc gaatcgactt
    14641 ggacgacgac ttcaccggct ttgacttcct ggcctacggc cagcggtgtc gatacgagtt
    14701 tccccgataa cttcggcacg atattggtca tatgtaaggc cgtaatgttg gcatccaggg
    14761 tcagggccac agggtggtcc gacttggaaa ccgtttcagt tttggtagac acgctgttgc
    14821 cgcagcccga aaggactgat acagccaagg cagccgtaca ggccaggacg gcccatttct
    14881 ttaatttcac gatatcccct cttcacgcag gttaaaaaaa cgtgtaatac aaggtaacca
    14941 gtatgacccc gggcaggccg aacaggccga tgatgaggcc tgtaccgacc gtaatcggaa
    15001 cgtgctggaa accaaaaaga tagccaaaat ggttgacgcc gtacaggacg atgagcccga
    15061 cgacgacgtt gaccactaaa tggcgcatag gcgccaagat gagcttatgc actaagaaca
    15121 ggacgaaaat gcctgcggct atccataaat attccataat cccctcacat ttcctgccga
    15181 ttggcaacag caccagccag ggttgcttca tcagcatact caatgtcgcc gcccatggga
    15241 aggccgcggg caatgcgggt cactcggatg ccggccgtct tgatgaggcg ggccagatat
    15301 aaagccgtag cttcgccttc cacgtcggga tccgtcgcca ggatgacttc cttgaccgtc
    15361 gtattgccca ggcgctgcag gagctcgcgg atgcgcaggt cttcgacatt gacgccatcc
    15421 aggggtgaca aggcaccgtg caggacgtgg tacagtccat gataatcgcc gctgcgttca
    15481 atggcctgga catcctgcgg cgtttcgacg acgcagatgg tcgagcggtc gcggctgatg
    15541 tcactgcaaa tcgggcacgg gtcctgggac gacaagttga agcagaccga gcaaaaatgc
    15601 atattctgct tggcatcgcg gatggcatcg acgaattcat cgacacggct gttgggctgt
    15661 tgaacgatat aataggccag cttgcgggcc gtcttgacgc cgatgccggg aaggcggcgg
    15721 aactgctcgg tcagcttatc taatggcgta tccattaaaa catgcccggc aggtgcatcc
    15781 cgcccgtgat tttgctcatt tcctgagcca tcatgtcgtc gactttcttg ttggcttcat
    15841 tgacggctgc gaccatgagg tcttccagca tttctgcatc ttccgggtcg atgatgctcg
    15901 ggtcgatgtg gacgctttcg acattctttt caccattcat gacgatagtc aggacgccgc
    15961 cgccgacggt agcttcgacg gtgcgcttct tcaaatcatc ctggagtttc ttcatttcct
    16021 tctgcatttt ctgtactttc ttcatcatac cttgcatatt tccaccaaac atgtgcgttt
    16081 cctcctaatt ttcgtgtaaa cttatttttt attttctata tatatattac agtctccta
//
LOCUS       DAWABZ010000032        16063 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_975_length_16063_cov_2.951462, whole genome shotgun sequence.
ACCESSION   DAWABZ010000032 DAWABZ010000000
VERSION     DAWABZ010000032.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 16063)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16063)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16063
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_975_length_16063_cov_2.951462"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    853..862
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7733..7742
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10522..10531
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13381..13390
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3169 a         4495 c         4981 g         3378 t
ORIGIN      
        1 ggaacagccg acggctgggg aaatcatcgt cgacggcatc agcatgagcg atgccaagag
       61 catcaacaat atccgcaagg aagtcggcat ggttttccag cgcttcaatc tcttcccgca
      121 catgacggtc ttgcagaaca tcatcttgtc gcccatgaag acccgcggtg aaagcaagga
      181 tgaagcgacg aagacggcta tccagctctt ggaaaaagtc ggcctggccg ataagaaaga
      241 cgcctatccc gaaactttgt ccggcggtca gcagcagcgt gtggccattg cccgcgccct
      301 ggccatgaaa ccgaagttca tgctcttcga tgaaccgaca tcggcattgg acccggaaat
      361 ggtccgcgaa gtattggacg tcatcaagaa tttggccaaa gacggcatgt ccatggccat
      421 cgtcacccac gaaatgggct ttgcccggga agtcgccgat cgcgtcctgt tcatcgacga
      481 agggaaaatc ctggaacaag ggaagccgga tgacatcttc ttccatccga aagaagaacg
      541 gacgaaactg ttcttgtcga agatattgta ggagaagtgc gccctagggg gcgcacgcgg
      601 ttcgcaaaaa gcggccagcg gccgcacgta ggggctcgca cgtggcgagc ccgcttccgc
      661 atatgcacga ttttcctaac cagtttacta ggagaatcat caaaattcca aacggaccgc
      721 ccacgtggcg acccacatca gacatcaaac tttcaactta aaactcaaaa aaagggctgt
      781 cgcattaagc aatttttgct tcgtgcgaca gccctttttg ccgtaggggt cgtccccaag
      841 ctttttcagc ccnnnnnnnn nnatggaagg cacccgacgg ttgctgaaac ggacagctga
      901 ggatggatgg cccgcttgtg ggaattccgc aacctgatac cgcaggccgc cgataaacct
      961 ctcagtcagc tgcgctgaca gctctcctag caggagagcc gggcgccctt tgggacggcc
     1021 cctacgatgc agccatcagg ctgcgagacc taaccactag ccactagcca cgaaccactg
     1081 ctttttcaaa ggctgtgcgg gcggcggtta tggttttttc gatgtcttcg tcgctgtgga
     1141 cgccggagac gaagttcgtt tcaaactggg acggtgccag gtagatgcct tgttccagca
     1201 tggacaggaa gtagcgctgg aaggcttcct ggtcactggc ttcggatgtg gcatagttgg
     1261 tgaccgggac ggcgctgaag aagacgctga acatggtgcc ggcccgatgg acctggacgg
     1321 cggcgccggc ttcgtcagca gcggctttga ggccgtctgt caggattttc gttttggcgc
     1381 aggctttttc gacgtaatcc gggtcttccg tgatgatgcg cagtgtttcc aatccggctg
     1441 ccatggccag gggattgccg ctgagggtac cggcctgata gaccgggccg gccggcgaga
     1501 cgcagtccat gatgtccttg cggccgccgt aggcagcgac gggcaggccg ccgccgatga
     1561 tcttgcccaa ggtcgtcatg tccgggacga tgtgatagat gctctgggcg ccgtgcaggg
     1621 ccgtgcggaa gccgcacatg acttcgtcga agatgaggac tgtgccgtgt ttcttcgttt
     1681 catcgcgcag gagctgcaag tagccgtctt ccggcaggac caggcccatg ttgccggcaa
     1741 tgggttcgac gatgacggcg gcaatgtcgt cgccgcagcg gtccatgagt tcccggaagg
     1801 cgtcggcatc gttataggcg acggtcagcg tgtctttggc cgtgccggct gtgacgcccg
     1861 ggctgtcagg ctgaccgaag gtagcggcgc cggaaccggc cttgaccagc aggctgtcgc
     1921 cgtggccgtg atagcagccg atgaatttca cgattttatc gcggcccgtg tagccccggg
     1981 cgacgcggat ggcgctcatc gtcgcttccg taccggagtt gaccatgcgc agtttttcga
     2041 tggacggata gatttctttg accaactggg ccagctgcga ttcaatcagc gtcggcgcac
     2101 catagctcgt gccgcgggtg gccgcttcct gcagggcctt gacgacgcgg ggatgggcgt
     2161 ggccgacgat catggggccc caggaaccga cgtagtcgat atactggttg ccgtcgatgt
     2221 cgcagatggt actgcctttg gcccaggaaa tgaacggcgg cgggcagccg atgcggttat
     2281 acgagcggac cgggctgttg acgccgcccg gcatgagggt ctgcgcttct tcaaaagcgg
     2341 cagctgattt ttctaagtga agcatggaaa ggcctcctta ttctttgatg tctaaattga
     2401 agacgttccg cagttcgcgg acgacccggt cttcgtcagg cgtgttggct gcttcaatcg
     2461 tgcggcgcag gggagcacgc aggattttgc ggaccaggat atgggataag ttctcgacga
     2521 cgcggaactg gtcatcggtc agttcgggca gtttgttgcg ggctcgtttg acgaaatggc
     2581 gccgtgtccg ttcagcctgc tgggacaggc tgaggacgac gggacggacc gacaggtatt
     2641 gatagcgttc aatcatggtg ttcacggctt cgtcgacgat atagctggcg gctacggctt
     2701 cgcgctggcg cgtagcttcg ttgtcgtgga cgacttcctg aaggtcgtcg atgttgtata
     2761 aggtgacgcc gggcagacgg gtcacggccg ggtcgacgtc gcgcggtacg gcgatgtcga
     2821 tgagcagcag gggccggccc tggcgtagtt cctggaagcg ctgcagccgg gggacggtga
     2881 tgacgtagtg cggtgccccc gtcgaggcga tgatgatgtc gacgtcggag acgacggact
     2941 ggacttcttt atagtggatg gcctggccgc cgacgtcagc ggccagttcc tgggcccgtt
     3001 ccagatggcg gttgacgatg tacagtttcc cggcgccttt gcctaagaaa ttctgcgccg
     3061 tcaggcgggc catctggccg gcgccgtaga gcaggagatt ccggccagac agggagccga
     3121 gctgtttttc ggccatctgg acggctgtat agctgacgga tacggcgttg taggcaatgt
     3181 gcgtttccgt ccggacccgc ttgcccgtgg cgatggcctg ctggaagagc atgttcagga
     3241 tggcactggt cgcgccgttt tcgtgggcca tgacgtaggc ctgtttgacc tggctgagga
     3301 tctgaccttc gccgaggacc agcgagtcca ggctggcagc gacgttgaac aagtggtgga
     3361 tgcaggcttt tcccgtatag ctgtagaaat aggacggttc ataagccgga cggccggctg
     3421 cgtcgaggaa gaagtcgcgc agggcctgtt cattatcgcg cacgtcgtcg acgacggcat
     3481 agacttcggc gcggttgcac gtcgacagga tgacggcttc gcgcaggccg ccgtaatcgt
     3541 cgagatgctg cagggcgtcg tggatggcgt cgggtgtcat agtgaagtgt tcccggatgg
     3601 cgacggggac ggtcttatga ttgaggccga ggatgacgag gtccatttta tgcctcgact
     3661 tcgccgtttt tcagccactt ggcgacgtcg acggcgtggt aggtgatgat gatatcggcg
     3721 ccggcgcgtt tcatgctggt cagttcttcc atgacgatgc gtttttcgtc gatccagccg
     3781 ttgcgggctg cggctttgac catggcgtat tcgccgctga cgttataggt cgcaatgggg
     3841 cgctggcagc gctggcgggc ctgatagacg atatccaggt aggaaagggc cggtttgacc
     3901 atgatgatgt cggcgccttc ttccaggtcg aggtcgattt ccttcatggc ttcgcggcca
     3961 ttggccgggt ccatctggta ctggcggcgg tcgccgaagt gcggtgccga atcggcggcg
     4021 tcgcggaagg ggccgtagaa gccggatgcg tatttggcgg cataggacat aataatcgta
     4081 ttgatgaagc cttcttcgtc cagggcctga cggatggatg cgatgcggcc gtccatcata
     4141 tccgacgggg cgacgatatc ggcaccggct tcggcctggc tgacggcgac tttgttgagc
     4201 agcttcagcg tcgcgtcgtt atcgacgtag tggtctttga cctgaccgca gtggccgtgg
     4261 tccgtgtact ggcagaggca gacgtcgccg acgatgacca tgtccggcag ggctttcttg
     4321 atggcgcgga tggcacattg gaccgggctc tgcgggtccc aggcgctgga gccgatttcg
     4381 tctttataag ccggcaggcc aaagacttcg atagcccgca atcccaaggc atagatgtct
     4441 tcggccaggc gtacggcgtt gtctacggac aggtgataac agccgggcat gctggggatt
     4501 tcttctttga cattcgtgcc ggggacgacg aagataggat aaatcaaatc gttgagacat
     4561 acatggtttt cccggaccat aacgcgcagg ggagcagaaa ggcgcaggcg gcgggggcgt
     4621 aaggtattca atttcatgac ttttggattc tccaatctgt aatacattca gccagaccgg
     4681 ctgtggtgta ggtctgggct gtgattattt ttttcaggcc tgcttcctgg caggtgcggg
     4741 ccgtgatggg accgatgcag gcaatggtga tgtcgtcgag gacgtcggtc ggggcctggt
     4801 tcagggccag gaagttctgg acggccgacg aactggtgaa cgtcacaacg tcgacggccc
     4861 ggcgcgtcaa gagatccgtc agggtctcgc tgttttcgct ggctgcgacg gtacagtagg
     4921 cctggcagat gtcgacgacg gcaccccagc ggcgcagggt gtcaggcagg acaggccggg
     4981 cttctttggc ccgcgggatg aggatgcgtt cttttcccga caaatgagcc tccagagcgg
     5041 cggccaggtc ttcggcacag taggcgtcgg ggacgacgtc ggcgcgaagg ccgtaccggt
     5101 tcagggcctc ggccgtagcg ctgccgatgg cggccagttt ggcccggccc agggcccggc
     5161 tgtcgcggcc gtggtgctgg aggcggtgga aaaaggcatc gacgccgttg gtgctggtga
     5221 agatgaccca gtcatagctg gctaatcggc cgatggcttc gtccagggcc tgataatcgt
     5281 cagtcggcgg cgtgatgcgg atggtcggga cttccaggca gcgggcgccc agttcttcca
     5341 ggcggtgtgt caaggccggt gcctgggcat gggtccgggt gacggcgatg gtcaggccga
     5401 agaggggctt ggtatcgaac cattgcatgg acggacgcag ggcgacgacg tcgccgacga
     5461 tgaagacagc tggtgcctgg aagtggcagg ctgccgcttt ttcagcgatg tcgccaaccg
     5521 tggctaccag cgtttcctgg tcggccttgg tgccccagcg gactagggcg gccggcgtgg
     5581 aagccgggcg gccgtaggtg atgagctgtt tggtaatgga cggcaggttg tggacgccca
     5641 tgaggaaaat cagggtatct acggccgtcg ccagtttgtc ccagtgaatc gatgaatggc
     5701 ccttggtcgg gtcttcgtgg cctgtgacga cggcaaagga agaggccgcc gtcctatcgg
     5761 tcacggggat gccggcataa gccggtgcgc cgatggccga tgtgacgcct gggatgattt
     5821 caaagggcag gccggcgtcg tgaagggccc ggctttcttc accgccgcgg ccgaagacga
     5881 aggggtcgcc gcctttgagg cggacgacgg tcttgccttc tttggctttg gcaatgagca
     5941 ggtcgatgat tttgtcctgc gataacgtat ggtcagcggc ttttttgccg acgtagattt
     6001 tttcagcacc tggcggggcg aattctaaaa ggcgcggatc ggccaagtag tcgtagatga
     6061 cgacgtcggc ccggcccagg acttcctggc cccgcagggt gagcaggcgg tagtcgccgg
     6121 gaccggcgcc ggtgagataa acgataccgt tcatggaaaa cctccttagt aagtcggcgt
     6181 aatgcctaaa tcggtgagga tgcggcggcc gccgtcatcg aggagcgtac aggccgcttc
     6241 tttgccgatg gcagcgctgt cggcgacggg accttgcgtc gtcgtgcggc agacctggct
     6301 gccgtctggg gcggcgatga tgctctgtaa ggtcagcgtc ccgttccgga ccgtgccgta
     6361 gacgccgacc ggaacctggc agctgccgtt gatggcggcc aggaaggccc gttctgctgt
     6421 cgcagcggcg gccgtgtcct ggtcgttgag gaaagccagt gcctgcctca gtgacatatc
     6481 gtcggcgcgg gtttcgatgg ccagggctcc ctggccgacg gcaggaatca tggtcgtgac
     6541 cggcaggagc tggcagatgc ggtcggcccg gcccaggcgc tgcaggccgg cttcggccag
     6601 gatgatggcg tcgtattgac cgtcatcgag ctttttcagg cgcgtatcga cgttgccccg
     6661 aaggtcgctg acgaccaggt ccggccgggt gtggagcagc tgggctctgc ggcgcaggct
     6721 cgatgtgccg atgcaggcgc cggccggcag ggattccagg gtggcatacg tattggaaac
     6781 gaaggcgtcg tggacgtggt ggcgggccgt gatggctgcc aggcagaggc cgtccgggac
     6841 gtcggctggc aggtctttca ggctgtggac ggccaggtcg atggtgccgt ccaggaggcg
     6901 ccgttccaat tccgtcgtaa acagcccctt gccgccgatg cgggctaagg ggacgtctag
     6961 gattttatcg ccttgcgtga cgatatgctg gagctggacg gacagctccg ggtcgtggcg
     7021 gcgcagggcg tcggcgacgt agttggcctg ccacagggcc agctggctgc gccgcgtacc
     7081 gatggtcaga ttacgtttca tggtttttcc tttcccaggc ggccagccga tcccggagca
     7141 gggcgctggc tgcgtcgatt tcgttattgt ctaccagggc catgacgtcg tccgtcaggg
     7201 cctggcgcca gaaggcctgg cggtcccggc tggtcggaag gacggactgc ccttggcggc
     7261 ggatagctgc taattcagcg agccagcggc catagcgttc gtcgaagtgc tgttccagtt
     7321 gcttccggag ccagcgcgac agcgccggtg ccttgccgtt ggtggaaatg gtgagctgca
     7381 ggtcgccctg gcggacgatg gacggcaggg tgaagttgca caaatccggg acgtcacaga
     7441 cgttgagcag tttcccctgg gctttggcag cagctgcgac ggcgtggctg acggcttcgt
     7501 tgtcagtggc acagatgatg aggaagaagg ttgactcatc accggcctgg tatgtccgcg
     7561 gctgccaggt caggtggtgg ctttcggcca gttcctgcag cggccgggtc agggacgggg
     7621 caatgaccgt cacggcagcc ccttcggcca gcaggcggca gaccttgcgt tcggcgacgg
     7681 cgccgccgcc gaggaccaga caggactggc cgttgagatc gaggttgatt ggnnnnnnnn
     7741 nntccttttt agaagtcagc ggctagctgt tagccgttag cagatggttt taaatttaaa
     7801 gccgttgtct ataaactgca aattccaaat ggtgacccct acactcaaaa actgctttgc
     7861 gtgcaataaa aagagcaacc tctcagtcag cttcgctgac agctctccta tgaagagagc
     7921 cagtggggca cgagagcggg ccgccctttg ggacggcccc tacgatttct tatccctacg
     7981 agcccttagc ggatgaagtt ggtcatgatt ttggcttctg ttgccggggc ttcgatgcct
     8041 gcttttttgc cggcttcgat gcatttcagg atccaagcca tgttgcggcc gagggtacgc
     8101 atgatctgca agccttcttc gtctttcttg acttcttccg gcgtgttgcc gtggaccatg
     8161 ggccagtagt tggatgcgac gctgaccatc tggtgataag ccaggtattt attgaggacg
     8221 tccagcgttg ccgtcgtgcc ggcacggcgg gccgaagcga cggtggcgcc gactttatgg
     8281 gtcagggcag cgccggcttt gccagccagt ttatccatga aggagatgat ttcaccagac
     8341 ggcgaagccc agtagaccgg cgagccgatg accaggccgt cagcagcctt cattttttca
     8401 gcgacagcgt cgacggtagc atcgtcggga acggcgtgga cgatttcagc atcgataccg
     8461 ttttctttga gcgtgtcggc gacgacggat aaagccgtat acgtgcagcc cttttcacgg
     8521 cggctgccat ttaataagat aactttcatg ggaatccctc ctgtaaatat catgattaca
     8581 tatagagatg atagaaaact accattcttg caaagatact atagcacaga aagggcaaaa
     8641 aagcaaatat gcaggcctcc gttcgccgtt cgccgttcgc aaaaccagcg gctagccgct
     8701 aactgctaac ggctggtttt tccccaggcg catagggtac cctgcttcta tgatataaaa
     8761 atatcaaaat ataaaattta aaatgatatt tttattgcat ttagtcaacg ggaatggtat
     8821 cataaagtca tcgaaaagat tccttaccaa gctacgtgaa agggagtatg actcatgaat
     8881 attattacgc ctatggattc ggcgaaccag gaagtgtgga tcggcgtcga cgtcggtacg
     8941 accggggtcc gggccattgc ttatacggaa gacgggacca acgtctgctc gtcggaagcc
     9001 ttttatccgc tgttgacacc tcatccggac tgggctgaag aaagcccgct ccagatcctc
     9061 gaagccgtcc aggaagtcat cagtaagacg gcggctcagc tgcgttataa gaacaaagaa
     9121 ctggcaggca tcgccttgag tacggtcatg cacagttttg ccggcctcga tgaaggcaga
     9181 gagcccctca tggacatgca gacctgggca gacagccgca gtgcggccat cgtccgggag
     9241 atgaagaaag acgaagcctt gtgccgctcc ttctatggac ggacgggctg ccccatccac
     9301 gcctgctatc ccctggccaa gatcatctgg ctgcgccaga atcagccgga actcttccgc
     9361 cagatgcgct atgtaggctc gctgaaggat tatttattct atcatttgac tggccaatgg
     9421 gtcatcgata agtctgctgc cagcacgagc ggcatgtaca acgaacagac tctggattgg
     9481 gacgatgaaa tcctggccta tgccggtatc accaaggacc agctgccgcc tgtcgtatcg
     9541 acgacgtaca gccaggggct ctgtgaagaa gcggccaaag ccctgcgcct gcctgttggc
     9601 cttcccgtcg tcatcggcgc tactgacggc gtcctggtca atgtcggcat cggagccgtc
     9661 gaaccgggcc agctcagcgg gaccatcggg accagcggcg ccctgcgcat gctgacccgc
     9721 cagcctaaga cagacccgca gatgcgcacg tggtgctata acctgaccga cgacatgtgg
     9781 gtcgccggcg gggccatcaa caacggcggc atgattctgc gctgggtccg cgacaagatc
     9841 tgccattacg gcggcagtgc cctggaaacg ctggacatcg acccttatga cctgatgacc
     9901 atgaaggcag aacacgtcga tgccggcgcc gacggcctca tctgcctgcc gtatttcacg
     9961 ggtgaacggg cgccgtactg gaattcggaa ctgcggggca tgttcttcgg cttttctcta
    10021 aatcacagcc ggtcccacat gatccgcgcc gtcatggaag gcatctgtta cagcctgaac
    10081 agcgtcatgg cagccctcaa ggaattcggc gacgtcaagg acatccgcgt cagcggcagt
    10141 ttcaccaagt cgaaactgtg gctccagatt ttatcagacg tcctcaacca gcccattacc
    10201 ctgccggaca acagcgaagg cgctgccttc ggggcggctg tgctgggctt catttccagc
    10261 ggcaggttga agggcatctc cgatacggcc gacctggtcc acgccaagaa ggtgtatacg
    10321 cctatggaag aaaacgttgc cgtctatcag cagctctatg acatcttcgt ccggctctat
    10381 cataacttgc agcgcgaatt tgccgacatc acggcatatc agcagaaatt ataatttcca
    10441 gtgaaaagga gcgattgcta tggcacttca cgatattggc gtcgtcggca tggccgtcat
    10501 gggcagtaac ctggccctga annnnnnnnn ngccgaccac ggctatgacg tttcggttta
    10561 caactatacg ccggacttga cggaacagtt cctccaagaa cggcctcatg aaaagattac
    10621 gggctacttc gagctgaaag atttcctggc ttccctgaag cggccgcgca agattatgct
    10681 catgatcatg gccggcgctc ccgtggacag catgctcgac cagctgctgc ctctcctcga
    10741 tacgggggac atcatcatcg acggcggcaa ttcctatttc ggcgatacgc gccgccgcta
    10801 cgaccgttgc agggaagacg gcatccactt ctacggcatg gggatttccg gtggggaaac
    10861 gggggcccgc cgcggcccgg ccatcatgcc tggcggcaat aaggaaacgt acccggaaat
    10921 ccagcccatt tacgaagcca ttgccgctaa agccgctgac ggcaagccgt gctgcacgta
    10981 tatcggcgaa gacggtgccg gccactatgt caagatggtc cacaacggca tcgaatacgc
    11041 cgacatgcag ctcattgccg aagcctattt gctgctcaaa tacgtcggcg gttacgacaa
    11101 cgccgctatt tccaagattt tccatgaatg gaaccagggc gagttgaaaa gcttcctcat
    11161 cggcatcgct gcagacatct tcgctgaaga cgacgaagcc ggcggacagg tcctggataa
    11221 gatcgtcgac gctgccggtc agaaggggac aggccgctgg acgagtatcg agtcaatgaa
    11281 acaaggcgtc gatatttcca tgatcaccgc agcctgcaat gcccgcgtca tgtccaatgc
    11341 actgggccgt gcgaaagcca ggacctgcat cgaaatgcct gccttgtccc atcagcacgg
    11401 tgatgacttc gtcgaagccg tccgccagag cctctatgcg gcgaaaatcg tcgcttatgc
    11461 ccagggcttt tccctctaca agagcgcgtc ggaaacgtat gactggcacc tcgattacgg
    11521 cgctatcgct tctattttcc gggctggctg catcatccag gccgaattcc tgaccaagat
    11581 taccgaagcc tatgacaaga atccggacct ggacaatctc ttgttcgatg acttcttcct
    11641 ggcaaagatt aacgccaatc agggcgccct gcggcagatc atcggcctgg ccatcgccaa
    11701 cggcctgcct atcccggctt tttcggcagc cctgcaatac ctcgatgcct acagcagtcc
    11761 ccaggtcggg gccaacctca tccaggccct gcgcgactac ttcggcgccc atacgttcca
    11821 gcgcgtcgat aaagaaggca ctttccatca tcattggcac gagcactata ccaaataaag
    11881 gaggcttctc attatgccac tcgtcatcct agccattggc attcttgtct tatttttcct
    11941 catcgtcaaa gtaaaactca atagtttcct ggctttgctc ctcgtcgccg gcgccgtcgg
    12001 cctggctgaa ggcatgccta tcgacaagct catcccgacc attgaaaagg ggctgggcgg
    12061 taccctaggc ggcctggcca tcgtcgtttc tttcggcgcc atgctgggca agctcatggc
    12121 tgaaagcggc ggcgctcagc gcatcgccac gacgctgatc aacgtcttcg gccgggaaaa
    12181 cgtcaaatgg gccgtctgcc tgacaggctt catcgtcggt atcgccttgt tctatgaaat
    12241 cggctttgtc ctgctcattc ctttagtctt caccattgcc gcagaagcca agattcctct
    12301 gctggaagtc ggtatcccta tggctgcggc cttgtcggtc acccacggct tcctgccgcc
    12361 tcatccgggc ccgacagcta tttccatcat ctataatgcc gatatcggca cgaccctggt
    12421 ctatggcgct atcatcgcca tcccgacggc catcgtcgcc ggtccgctgt actgcacgct
    12481 gacgcgcgac atcaatccgg aaatccccaa gggcatgtac aacccgaaaa tctttaaaga
    12541 cgacgaaatg cccggcttcg gcatctccgt cttcacggcg ctggtcccgg tcatcctcat
    12601 ggctgcttcg gcgattgcca aattgactat gggtgccgat gtcccggccc gtcacgtcct
    12661 gaccttcttc ggccagccgg acatcgccct gaccatttct gtcgccatcg ctatctatac
    12721 gttcggcctg ggacggggta aatccatgga agacgtcatg aagacggtcc aggatgccgt
    12781 cctgaccatt gctatgatct tgctcatcgt cggcggtggc ggtgccttga aacaggtcct
    12841 catcgacagc ggtgtcggca cgtatatcgg caacctcgtc gccggttctg atttatcgcc
    12901 gctgctcctg gcctggctgg tcgctgccgt catccgcacg gcctgcggtt cggctacggt
    12961 tgcagccctg acagccggcg gtatcgcagc gcctatctgc gccgtcaccg gtgccaatcc
    13021 ggaactgatg gtcctggcaa ccggcgccgg ttcgctcatc ttcagcccgc cgaatgaccc
    13081 cggtttctgg ctcttcaagg aattcttcgg cctgacagtc aaacagacgg tccgcacgtg
    13141 gtgcgctttg gaaacgatca tcgccgtcat gggcctggcc ggtgtccttg tattgaacat
    13201 ctttgtaggc taaaataaaa gtaaatacag aagtgggagg gaagacaatg gaagaaacga
    13261 aactccagtt atttcctgtc ctgcgcagtg catacggcgg tctgaccaag tcagagcgcc
    13321 gtattgccga ttatatcgtc gatcatacgg acgaggtcat ggaacagacc gtttcggacc
    13381 nnnnnnnnnn ttcatgcgca gagttcggaa atcactattt cccggttttg caagaaattg
    13441 ggatgcagcg gcctgaagga attgaagctc atgctggctg cggaattatc ggcggcgccg
    13501 cagaaggcct tccacgacat ccagaccggc gatagcggac gggacgtagc ggccaaggtc
    13561 tttgccaata ttgccgaagg attgcaggat acgctgagcc tcctcgatta tggcgctctc
    13621 gatgcggcgg cccggctgct cttgaaagcc cggcgcatct acgtctttgg ctttggcaac
    13681 tcggcgacgg tctgcatgga tatggccacg cgtttcctgc gcctgggact ggctatccgg
    13741 gagtatgccg actcccatat gcaggtcacg gcggcggccc tgatgacggc cgacgatgtg
    13801 gtcatcgccg tatcccatag cggggcgtcg aaggaactat tgcaatcggt ccaggccgcc
    13861 agggagaacg gggccaaagt catcgtcatc accagccatg gccagtcgcc tttggctgcg
    13921 ttagccgata tctgcctgtg cggcatgggg cgcgaagtcc agtacacgtc agaagccggg
    13981 gcgtcgcgcc tgatccacat ggccatcggc gatgtcctct atacgcgcgt ggccatgcag
    14041 gaaaaggcgg tctttcagga caatatggaa aagatgcgcc gcgaaatcgt caaaaagcgg
    14101 cagcactgat tttggctacg aaaatcggcc gttctgtggt acaataaaac tgttcttatt
    14161 gcaaacagag gaaggatgat tagcaggtga agaaattctt ggaaatggcg actatcgtcg
    14221 agaatgaagc cattattcct gaagtatgga aaatggtatt ccatgcaccg cagattgccc
    14281 gggaagcgca gcccggccag tttgtcaatg tccatcacga tgggggacag acctttttgc
    14341 gccggccttt tggcatcgtc gatgccgatg ccgaaacggg tcatgtcacc atcatttacc
    14401 gcctggtcgg caagggcaca gaagaaatga agggcctgcg cagcggtgac gcattgagcg
    14461 tcgaagggcc cctgggcgaa ggcgtcttta cgacaacgcc gggccaggtc ctgctggccg
    14521 gcggcggtgt cggcctggct ccgctcatct ttttggctaa gcgcctggac catcccatcg
    14581 tgctcgttgc cggcaagacg gcggctgaaa cgttctggac caagttcttt gaaccctatg
    14641 ccggccagat ttacgtgacc actgacgacg gctccctggg tatcaagggc tttgccgtcg
    14701 atgccctgcc gcagatcttt gcagaacacg ccatcgaccg cgtatcggtc tgcgggccga
    14761 cggtcatgat gaagaccatc gcccaggcgt cggccaaagc cggcgtcgcc tgtgaagtgt
    14821 ccatggaaaa gcggatggcc tgcggtatcg gcgtctgcct gggctgcacc tttgaaagcc
    14881 agctcgacgg caaacgctat aaagtctgtg ccgacggccc cgtatttgac gcgaaggagg
    14941 tctttgcatg atcagcgaac gcattgccgt ccagtttgcc ggtatttcca tgaagacacc
    15001 ggtcatggga gcatcgggga cctttggatt tggcgtagaa tacagtgatt ttctcgacct
    15061 cgatcacgtc ggggccatta tttccaaggg catcacgccg ctgccgcggc cgggcaatcc
    15121 cggcgtccgc gtggccgaaa cgccggcagg gatgctcaac tgcatcggcc tggaaaatcc
    15181 cggcgtcgaa gtcttcaagc gggatatcct gcccaaggtc cagcaggcct gccagacgcc
    15241 gctcatcgtc aatatatcgg ccgggactgc cgaagaatac ggcgaattgg cagctcagct
    15301 cgacgtcgac ggcgtggctg ccctggaagt caatatttcc tgtcctaacg tcaaagaagg
    15361 gggcatcgtc ttcgggaccc agccggaagc ggctgcggct gtgacgcgca gtgtcaagga
    15421 ccatacctgg aaaccggtca tcgtcaaact gtcgccaaac gtcacggaca ttacggtcat
    15481 ggctaaagct gtcgaagcgg ccggtgccga tgccgtatcg ctcatcaata ccctgacggg
    15541 catggccatc gacatcaaga cccgccgccc cttgctgggc aatatcaccg gcggcctgtc
    15601 cggcccggct gtcaagcccg tcgccctgcg catggtatgg cagacggctc aggccgtatc
    15661 catccccatc ctcggcctgg gcggcatatc gacctggcag gatgccgtcg aattcatgct
    15721 ggccggtgcc agtgccgtcg ccgtcggcgc ccataacttc gtcgacccca gagccgtcga
    15781 aaccatcgcc gaaggcctgg accagtattg ccgagaagaa ggcatcgaac atatacagga
    15841 tattgtaggg gcattggaga ttcgtggcta gtggctaggg attagcagtc agcggtcagc
    15901 cgtcagcagt tagccgcggt cccatggacc gctttgtagg ggccgtccca aagagcggcc
    15961 cgttgtggga atcccattca cgagctgccc tggatatatg ccaagacatt tatataccca
    16021 tttgcgtgga cgggcgcccc acgtggggcc cctacagaaa aaa
//
LOCUS       DAWABZ010000033        15884 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_1000_length_15884_cov_2.738834, whole genome shotgun sequence.
ACCESSION   DAWABZ010000033 DAWABZ010000000
VERSION     DAWABZ010000033.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 15884)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15884)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15884
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1000_length_15884_cov_2.738834"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2581..2590
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4686..4695
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6893..6902
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3156 a         4238 c         4410 g         4050 t
ORIGIN      
        1 tcgacgagga ggatatccat gcccttggct ttggccgacg ccgtggcatc gaagacgacg
       61 gcggctgcat cggcgccttc ctgatgcttg acgatgggta cgccgacgcg gtccgcccag
      121 atggtcagct gttccgacgc tgccgcacgg aaggtatcgc cggcggcgag catgaccgat
      181 ttcccctgct gcttgtaata cttggccagt ttgccgatag tcgtcgtctt gccgacgccg
      241 ttgacgccga cgatgaagat gacccgcggc ccgtctgttt ccggcaggtg ttcatcgttt
      301 tcattgagca gggcgacgat gcatttttcc aaatacggga cgacgtcgtt gccgtctttg
      361 atttccttcg atttgacacc ggtccggatc tggtccagga ggtattccgt cgtttcgacg
      421 ccaatatcgc cggtgagcat gaccgcttgc aggtcatcgt acatgtcgtc gtcgatcttg
      481 gcatagccgc ggacgacggt ttcgatattc tggatgagcg agtgtttcgt cttttctaag
      541 cctttgcgca gttttgaaaa aaatcccata gttatgttcc ttcctttaac aattcatcta
      601 ctttgacagt cagcaggcgc gatacgcctt tttcttccat ggtcacgccc aacagggtat
      661 tggccgcttc catggtcttg cggcggtggg tgatgacgat gaactgcgtc gcaccgctgt
      721 aattcttgag gtatcgggcc atgcgttcga cattggcttc gtccagggcg gcgtcgactt
      781 cgtcgaccag gcagaacggt gccggatgat aggccagaaa agccaggagc agggcaatga
      841 ccgtcagggc ccgttcgccg ccggagagaa gcgtcaggga ctgctgcttc tttcctggcg
      901 ggcggatata gatttccacc ccggcttcca ggatatgttc cttgtcggtc agggccaggt
      961 gggccgtacc gccgccgaag agacggctga agatatcctg gaagtggacc ccgatttcct
     1021 tgaaggcctt ggcgaattgt tcggccatgg ccgcgtcgat ttcagcgacg acagtccgca
     1081 gtttttcccg cgattctttc aggtcatcgc actggcggtt gtagaagtcc cgcttttcca
     1141 gagccgtctt gtattcgtct tcagctgccg ggttgatggt ccccagggct gtgatctgcc
     1201 ctttcaggga ggcgaccttg tcgtggagct ctttcagcga gccttcgcgg cggcgctcca
     1261 tggcttcctg gcggctcagg ccctgcatgg ccagccgttc ttcgtgatgg ctgatatcgc
     1321 tcttgtattt ttcccgctgg atgtcggcag cgttgcagcg ctgctgccaa tcctgatggc
     1381 gccggcgcag gtcgtcgacg acaccatcca ggtcctggct ctgtttcaag gactcattgc
     1441 gggtctgata aaaagcctct ttggcctggt cgcagcggac cgtatcggct tctttcaagg
     1501 taatattttt cgacagttct gtcaacaggc gggccgcttc ttcctggcgg cggcgcaaat
     1561 cctggcggcg ctgggccagg gcctgttcct gtaccgtcag ttcctgctgc catttgtcat
     1621 gctggcgcag ctggtcgtcg ccatggctgg cctgttctgt cagtttggct acggcaatct
     1681 gcctttccgt caaggtctgg cggcactggg cggcttcggc cttggcagca tcacaggcgt
     1741 cctgccattg ctggacatcg gcggccggcc ccgtttccat atcggccagg gcggcttcct
     1801 tggcgaccag ccgggcctgc atgtcggccc gttccttttc caaggcttcg acgcgctggt
     1861 caaaatcagc cagggcctgg cggatatcgg ccaggccctt ctcttcctga ccgccctgcc
     1921 agcgggcctg ttccgcagcc tggctggctt tgcgccaatg gtcttcggcc tgggccagtt
     1981 cccgggcaga ttcttcggcc tgccggcgca gttcccggcc ctcagcggcc agggtttcca
     2041 gacgctgctg gccacggcgg caggtctcct gcaattcctg cagcagcgcc cgccggctga
     2101 tgagggaacc ttcctgattg cgggcgctgc cgccggtcag ggagccgccg gcgttgaact
     2161 gcgtcccgtc gaggcagacg atgcgcaggc gatggccgta tttccgggcc acagccgagc
     2221 ccgtatccat ggtatcggcc aggagggtcc gccctaagag ggatgaaaag accttctcat
     2281 atttagcatc ataagaaata gcatcggagg cgaagccgag catgcccggt tcgccggcag
     2341 cctgttcttc atcggctgtc cgcagccggc tgcgcagggt atccaggggc aggaaggtcg
     2401 tccggccggc ttttctgtct tttaaatacg aaatagcctg tttggccgcc ctttcgtctt
     2461 cggcgacgac gtaacggacg gcaccgccca gggcgacgtc gatggcgacg gcaaagcgcg
     2521 atgggatctg gcagagttcg ccgacggcgc cgcacagccg ggtgcgccag ggctggctgg
     2581 nnnnnnnnnn cgtcaggacg actttgaccg cccgtcccag gccttcatgt tcctgttcca
     2641 tgctctggag gacgcgcatg cgctgggagg cggcgtcgac atcgccgcgc aggcggcggt
     2701 attcatcttc tccttttttc aggcgctggc ccttttcctg agccgtctgt cgcagcgagg
     2761 cggcctgctt ttcaaaggcg gcggcttttt tctggcacgc ttcctgttgg gcccgggctt
     2821 cctcatggcg ggcttctgcc gctgtcaggg cctgcttctt gtcttcccac tgcttcaggg
     2881 cctgctggca ggcggcttca ttttcttcca ggcggcggcg caggtcttca ctgtcccggc
     2941 gcagcatgaa agcgtcgcgg ctgcgcttgg agttggcgtc gacccgttcc tgcagggctt
     3001 tggctgccgc ttcggccttg gcttcggcct ggccgaacag ggtctgagtc agggctaaag
     3061 ccttctgagc cgcagccagt tcagctgctt tctggtcctt ctgggcctgc agtgtttctt
     3121 tttgttcctg ccaggctgcc tggcgctgct gcagctgagc ggcgtcgtca gcggcctggg
     3181 attcgttctc ggccagttcg gcctgccgct gccggcaggc ttcacggcgg tggacgaggc
     3241 tgtccagctc gttgtggacg gccatggctt cttcatcgag gcggcgcagt ttttcgtcgt
     3301 ctgccgccat gacctccaac agggccttgc gttttccctc ggccgctgcc agttcctggg
     3361 cggccttttc ttcttctgtc gccgccgtca tgcggctgtt ctcggctttg gccaggaggc
     3421 gttcgctgtt gcgcagttcc tgcaaggtca cggtcccttc ataggccagg cgctcgctgt
     3481 cgaggcagcg gaatttctgc agagtttctg cctgttcctg caatggttcc agccgctctt
     3541 cgaggacgct catgaggtcg cggatgcgct ccaggttgcg ctcggtctcg gcgatcttgc
     3601 gcaggccgtc ggccttgcgg cctttgaagc ggctgatgcc agcaacttct tcaaagatga
     3661 cccgccgctc ttcgggcttg ctgttgagga tgcggtcgac gcggttctgg ccgatgacgg
     3721 ccatagagtc ctggccgata cccgtatcgg cgaagaggat atggatatct ttcaggcggc
     3781 acggccggcg gttgatatag tattcactgt cgccggagcg gaagaggcgc cgcgtgacga
     3841 cgacttccgt gaattccgta tccaaggcgt ggtcgctgtt atcgaagtac agcgagactt
     3901 ccgctgcccc ctggggccgg cgggtctggg tcccggcaaa gatgatgtct tccgacttct
     3961 gcccccgcag ctggcggaca ttctgttccc ccatgaccca gcggacggca tcggaaatat
     4021 tgctcttgcc gctgccattg ggaccgacga tggccgtaat gcccttgtcg aaggtcagga
     4081 tcgtcttatc tgcaaaggac ttgaatcccc gcagttccat tttcaataat tgcatagttt
     4141 ctactctctc tgtggactgc ctggacggac acggtatggc gccgcatagc agttctgctt
     4201 atttactcgt acagttaatg ataccataat taccagcctg ccgccaccac ggcgtcgcca
     4261 gccagggctg ggcaaagttt gtgtaaaata tcagggaaag ccttgacaag tcctgtctta
     4321 atggattata ttagaattta ggtttgaagg ccctcattct gcagaaaggg agagtaccgt
     4381 aatgattcaa gtttataagc acagcaacgg ccatctggaa gacaacattt ccctggcatc
     4441 ggctgaaaaa ggatcctgga tccacgtcgt aaatcccgat tccgaagatt tacagctcgt
     4501 atccatggtc acggaaatcc ccactgatgt tctgaaaacc gccttggaca cggaagaacg
     4561 ttcccacgtc gaaatcgaag ataactacat ctttgtcgtc atcaacatcc cgatcatctt
     4621 ggaaacggac agttatgaca ccttgcccct gggcattttc attacaccgg acttcatcat
     4681 cacccnnnnn nnnnnccctc tgtattcaag aaacagaagt catgcgcgcc tttacggaga
     4741 ataagtatcc tctgttctat acattcaaga agacgcgttt cttattccag atcctgtacc
     4801 gttcggcgac gctgttcctg cgctacttga tgcagatcaa ccgccggacc gatgacatcg
     4861 aggaaatcct gcgccattcc atgcgcaaca aggaattctt cctgctcctg gaattacaga
     4921 aatccttgac gtacttcacg tcggccctgc gcagcaatgg catcgtcatg gaacggctca
     4981 tgcgcctgcg ccgcaatacg tcgctccacc acctcatcaa gatgtatgaa gaagacgaag
     5041 acctcctgga agatgtcatc atcgaaaaca agcaggctat cgaaatggtc gagatgtatt
     5101 ccaacatcct catgaatatg tccgatacct ttgcatccat catttccaac aacctgaact
     5161 tggtcatgaa attcctggct tccattacaa tcatgctggc cgtcccgacg atcatcttca
     5221 gcctgtgggg catcaacgtc cccgtcccct tccaggacac gacagccggc ttctattacg
     5281 tcgtaggcat cgccatgttc tgcaccgtcg tcgccgtcat catgctctgg cggaaggatt
     5341 tattctgaaa ggcccttcac catgctcttt atcggttcaa aagagcggcg gtgaatggga
     5401 cagggcccgt atttttgcaa cgcttccata tgcattttcg tcaaatatcc cttgtgcacg
     5461 gcaaagccgt actggggata ttttttgtcg tattcgacca tgaggcggtc acgcgtgact
     5521 ttggcgatga tcgaagcggc ggcgatggac gcgctcaggg cgtcgccgtg gaccagggac
     5581 ttatggggca cggtcagttc ccgaagaggc atggcatcga ccaggacggc ttcagccggc
     5641 acggccaggc cgttcacggc gtcgtacatg ccctgcatgg tggcgtggta aatatcgagt
     5701 tcgtcgattt ctttttccga cttggccaca caggatacgg caacggcttc ggccataatt
     5761 ttatcataga ggacgtcccg cttggccggc gggacttttt tgctgtcatt gaggccggga
     5821 cattcccagt agggcggcag gatgacggcg gctaccatga ccggaccggc agccgggccg
     5881 cggccggctt cgtcgatgcc ggctacgtgg tacagccctt ggttgtagta agccgtttcc
     5941 tgggcataca tgtgcaggac ccgttcccgt tcggcttctt cccggatttt ctggcgcata
     6001 taggcggcag ccagcttgcg gaccgacacg cggccgtctt ctttcatggc tgccaggagg
     6061 tcctggacgt caccgccagc ggccaggcgg gccttgaggt cggcgacgcg gagtttctcg
     6121 atgtccatgg tcagtcctcc ccttcccatt cgcccagccg gtccagggag atggcgccga
     6181 tcttgccttc gcggaattcc ttgaggatga gcttgtacgt cttttccagg tcgacgacgc
     6241 cgccggaaac gatacagccc cgcttgcggc cgatggcttc gatcgtatcc agcggcttgt
     6301 cacagagggt ttccagttta taacgggcct tcaattcatc agggtactgg gccgataact
     6361 gttcgatgag ctgcatgatg accgtttcca tgtcgaagac cgtatcggaa atggcccctg
     6421 tcgctgccag gcggtaggcg ctgacctggt tttccagttt cggccacaag acgcccggcg
     6481 tatccaggag gtcgagctgg tcgccgatgt ggacccactg cttgcccttc gtttcgccag
     6541 gcttgtcggc cgtcttggcc gcactgcggc gggacagctt gttgatgagc gtcgatttgc
     6601 cgacattagg aatgcccagg atcatggtgc ggacggcgcg gttcttgagg ccccgggcct
     6661 tccagcgggc cagcttcggc gctgccaggg agcggaccag ggaaatgagc tgtttcaccc
     6721 ctttgccgcc tttgctgttc agggccagga ctttgcggcc ctgctgcttg taataggcca
     6781 cccattcgtc gaggacggcc gggtcggcca ggtcggcctt gttgaggacg acgacagacg
     6841 gcttgtcgcc gatgagggac ttgatcatgg gattggtgct gctcataggg atnnnnnnnn
     6901 nncccttttt agttgttagt ggctagttgt tagtttttag tagatggctt tattttaaaa
     6961 cttctaactg caaactacaa actcacgcta ttagccttct cttgtttcgg tgagtttctg
     7021 gatgtgggcg acgacttcgt cgatcggttt ggcgcctaaa tcgcctttgg agcggtcgcg
     7081 caggccgagg gtgccgtttt ccatttcttt atcgccgacg acagccatgt aaggaacttt
     7141 ttccatctga gccttgcgga tcttgtagcc gatcttttcg ctgctttcat cgacttcgac
     7201 gcggatgccc aatttcttga acttcttagc caggtctttg cagtaagcca ggtgtttgtc
     7261 ggagatgggc aggaacttga tctggaccgg agccagccat accgggaaag caccggcata
     7321 gttttcggta atgatgccca ggaaacgttc gatactgccg aagcaggcac gatgtaccat
     7381 gaccgggcga tgtttctggc catcttcgcc gatgtaggtc atgtcgaagc gttccggcat
     7441 gagcatgtcg agctggatag taccgcactg ccaggtacgg ccgatggagt ctttcaggtg
     7501 gaagtccagt ttcgggccgt agaatgcgcc gtcgccttcg ttgatgacgt acgggatgtg
     7561 gcgttcttcg atgacatcat gcaaggtctt ggtagccagt tcccagattt cgtcggagcc
     7621 catggagtct tccggacgag tggacaattc gacgtgatat tccaggccga actgtttgta
     7681 agccttgtcg aagaggtcca tgacgccgga gatttcttct ttcatctggc tcggcagcat
     7741 gaagatgtga gcatcgtcct gagtgaagca gcggacgcgg aacaggccgt ggagggcccc
     7801 atggagttca tggcgatgga ccaggccgag ttcagccatg cgcatcggga agtcgcggta
     7861 gctgtggacg ttactcttat agaccaggat gccgcccggg cagttcatcg gtttgatggc
     7921 gtaaggctgt tcatcgatgg tcgtgaaata catgttgtcg cggtagtgat cccagtggcc
     7981 ggactgttcc cagaggtgac ggttgaggat catcggggtc atgatttcgt cgtaatcggc
     8041 ttcatcatgg agttcatgcc agaatttaag caagttgttg cgcaggatca ttcccttggg
     8101 atggaagaac gggaagcccg ggccttcttc gtggaagctg aacaggtcga gctgtttgcc
     8161 cagtttacga tggtcgcgct tttcagcttc ttccatcatc ttcaggtatt catcgagttc
     8221 cgatttcttt tcaaaagcgg tcccgtaaat acgctggagc atcttgttgt gttcatcgcc
     8281 gcgccagtaa gcaccggcaa cggtgcgcag cctgaaggcc ttgacggcac cggtcgaagc
     8341 gcagtgcggg ccggcacaga ggtcgacgaa gtcgccctgg cggtagatgg agatgacggc
     8401 atcttccgga aggtcttcga tgagttctac tttataatcc tggccagcgt ctttgaagag
     8461 tttcaaagcg tcggcacggg acaagacttc tttggtaatg ggaaggtttt ctttgacgat
     8521 cttagccatt tcagcttcaa tggcagcaaa gtcttcctgt gtgaaacggt gttctgtgtc
     8581 gaggtcgtag tagaagccgt tttcaatgga cgggccaatg gcgaacttga cgcccgggaa
     8641 gaggtgctgg atagcctgtg ccatgacatg agatgctgta tggcggagcg tatttttccc
     8701 ttcctggtcg gcgaaggtga ggaattcaac ggtcgagccg tcgacgatgg tttcgcggag
     8761 gtccgtgact tcaccgttta cctttgcgac taatactttc tttgcaagac tgttgctgag
     8821 ctgtttgaca gcttcggcaa gagagatgcc atcgggaaac tgttttttgc ttccgtcttt
     8881 taatactaat tctgccatta caaacatcct ccttaataaa aaaagctctc gtcctcaagg
     8941 gacgagagca taactcacgt ggttccaccc tagttcaccg gaaaacccga tgtactcaag
     9001 cgcgataacg gggcggccgg aaccccctac ttacttcggg aatcctgctc caaggcggta
     9061 acttgccaga ctttataaag cactctcacc atcgtgcctc tctctgtaat aaaggagcta
     9121 tcaagtgttg tccttttcaa agcatcttac atatctctat gtatgcgtaa attatacggc
     9181 aggaaatggg ggttgtcaag agagaagagg ctgtcttaaa gcgacagcct ctttttgagt
     9241 ttgtaggggt tgcccgtggg cgacccgcca agtatttgta tgatgtttct agcaacttga
     9301 ataggaaacc acacatatgc tgaaacgggt tcgccacgtg cgaaccccta cgaactagtg
     9361 gtcagacact aacggccagc tgccagtagc aaaaaaggtc tatcgcatag cgatagacct
     9421 tttgattatg cacggttaga taacttatgc aatgcctttc tgtttcttga tagcttccgt
     9481 gaggagcggg agaactttga agacatcgcc tacgatacca tagtcgcaga ctttgaaaat
     9541 cggagcatct tcgtctttgt tgatggcgat gatgcagtca gaaccagtca tgcctgccaa
     9601 gtgctggata gcaccggaaa tagcgcatgc aatgtagatc ttcggaccaa ctgttttacc
     9661 agtctggcca acctgatgga gagcatcgat ccagccagcg tcaacagctg cacgggatgc
     9721 accaacagcg ccgccgagga cgtctgcgca ttctttgagg ataccggtct tgaacggttc
     9781 ttcaccgttc atgccacggc cgccagcaac gataacgtca gcgtcttcga ttttaacgcc
     9841 ttcgccgccc aatttgatga gttcgacgaa tttggtcatg aagtcagcat cggagaggtt
     9901 agctttcttt tcgataactt cgccagttgc agaaggatct gcttctggtt ttttgaagac
     9961 gtgcgggcgg acagtaccca tctgcggacg gttgtccggg cagatgattt cagccatgat
    10021 gttgccgccg agagccggac gggtccatac gatggtcttc ttgtcgtcgt caacgccgag
    10081 ctgtgtgcag tcagcggtga ggccagtctt caaggagtta gcgacacgcg ggccgaggtc
    10141 acggccgatg ttggtagcgc caacgaggaa tacgttcggt ttttcttcat ggaagaaatc
    10201 gcaaataact tttgcataag ccgtcgtatt gtaatgttcg agctgaggat catcatatac
    10261 gaatacctta tgagcaccgt gagcgatgag ttcctgagcc aacggtttta cgtctttacc
    10321 aatgaggatt gcaccgactt cgtcgccgat ggtgtcagcc aagatgcgag cctggcccaa
    10381 caattcgaaa gatacatcac gtaatttgcc ttcaaactgt tcagcaatta cataaatgcc
    10441 tttatattct gctaaatcca ttttcactgt tcctccttag attaaatgat tttctgggca
    10501 accagttttt ccataatctg gtcgacgatt gcctgttcgt tgtcatcttc aactttgaga
    10561 acgagaccac cggatctctg aggcggtgtg aaaatcttgc ggactttagt cggagagcca
    10621 gacaaaccga tctgagcgtc atcagctgca acagcagcag cgtccaaatt cgggatttcg
    10681 cggcgttttg ctttcatggt gccacgaatg gtcgggaaac gcggttcgtt caagtcttta
    10741 acagcggtaa tcaataccgg gaagttagct tccgttacga tgtagccttc ttcgttttcc
    10801 tgctgaacag taactttgtt gccttcgact ttgatgtcgc gggcatacgt aatctgagga
    10861 ataccgagtt cgcaagcgat ctgcgggcca acctgagcgg tatcgccgtc gatagcctgt
    10921 ttgccgcaaa ggatgatatc gaactgttcg atacctttgt cagcagctat ttttttgata
    10981 gcctgtgcca aagcatagcc cgtagctaac gtgtcggaac caccgagttt gcggtcggac
    11041 agaagatatg catcgtcagc gcccattgcg aggccttcac gaagaacgtc ttttgcctga
    11101 tcaggaccca tcgagagtaa cgtaatcttt acgtctttgt cagcatcttt catctgaaga
    11161 gctgcttcca gagcattctg gtcgaagggg ttaaaaatgt taggaacacc agcacgaatg
    11221 acggtatgtt ttacggggtc aatcttaact tctgctgtgt ccggaacttg tttgacacat
    11281 aacaatattt ccatttacgt ttgcctccta aaatcttttt tatgtaacag gcttggctga
    11341 tgccaagcct gcatcataaa cttcacttat cttaacagag caccgcctgt aaccatgagc
    11401 tgaacttcgt tcgtgccttc gtagatctga gtgatcttag catcgcgcat gtgacgagca
    11461 accggatatt cttcgctgta gccatagccg ccgaaaatct ggactgcttc tgtcgttacg
    11521 cgcatagcaa catcggaagc aacgcgtttt gcgatagcag catcaacggt gaagggttta
    11581 ccttcctgtt tcttgcaagc agctttgtaa acgaggttac gggcagcttc gatctgcatc
    11641 ttcatgtcag ccagtttgaa ggaaatggac tggaatttgc agagcggttt gccgaactgt
    11701 acacgctgtt tggagtattc aacagcatct gccagtgcag cttctgcaat gccgagagcc
    11761 tgagcagcaa cgccgatacg gccgccgtcc aaagtcatca tagcgatctt gaagcctttg
    11821 ccttcttcgc cgagcatgtt ttcagccgga actttgacgt cctggaatac gagttccatg
    11881 gtctgcgaag tatggatgcc catcttgtct tctttcttac cgaaagtgaa gccaggcatg
    11941 ccgtcttcga ggatgaaggc tgtaatgccg tggttgcctt tgctcttgtc ggtcatagcg
    12001 aagatgatgt agatatcagc agcgccgccg ttggtgatga aaatcttgga gccgttcagc
    12061 gtgtatgtgc cgtcgtcgtt cttaacagca acagtctgct ggccggaagc atcaggacca
    12121 gcgttcggtt cggtcaagcc gaaagcaccg agtttagtac cttcaaccaa aggaacgagg
    12181 aatttttctt tctgagcttc tgtaccgaac tgccaaatcg ggttagcgca aagggaaacc
    12241 gttgccgaca gggtgatagc aacaccagcg tcgtatttag ccaattcttc aacagcgagg
    12301 atgtagctca aaacgtcgcc gccgtcatcg ccggaaccgc cgtatttttc ttcgaagtaa
    12361 gcgccggtaa taccgaggct gagcagttca tcaataaggt ctttgtcata aatacctttg
    12421 tggtcgcgtt ccgtaatggt cggagctaat ttcttttcgc cgaaatcatg agcgagtttt
    12481 aagaagtcct gttgaatatc tgttaagtta aaatccatga tgtttccact ctccttagtg
    12541 tatagttagt ttagtaaagc cataagataa aaacctgaac atcgaggcga tacatttcct
    12601 tacagtaaat tctgtctgga tatatattca caccgtctca gtgcttacaa atgcattgta
    12661 caacttttca catgttaaat caatagcaaa aagcctataa agcacaatat cactattcca
    12721 ttttctcatc ctaaaacttt aacaagactt ttttgtacta atggaggcaa aaagattaaa
    12781 atcttcttga cagagtacgc cttgcttttc cgtatacgct tttgtacgta tacctaacta
    12841 tactggttac aaatattaag gaaagcttac ggttgtatta tagcacggat accccggaag
    12901 ttgtcaagga tgtgtgccgt aattccccag attttataat tttcatacgt ataaatcttg
    12961 acgttatacg tccggcgctg tttccagcct gtctgatggg aaatatccag gcccttggga
    13021 aagcccggtg ccggccgggt agccagttcg acgcggccga tttccggttc atgctcatcg
    13081 aaccaggaca gagggaccgt aaaggtatgg tccacttcgc cccggttgag gacgaagtca
    13141 tctctatcca tataggcggc aaagggccat acggtgacgc ctacaggtga ttccatgtag
    13201 tccagggggc cccagacgtg gatatgggat gggtcgacgc cgagttcctc cttcgtctcc
    13261 cgcagggccg cttccagagc gctgtcgtcg ccgtcgtcga tgcggccgcc ggggaagcag
    13321 acgtcgccag gctgccagcc cagcttgctg ctgcgcactt caaacacggc ctgttcttcc
    13381 ccgtctttgt agataatcgg cactaagacg gcacaagccc gcgtattcat acaggtgttc
    13441 tggcggacga tacggtccgg ccatggctta aatgctttca tacaaggctc ctttctgagg
    13501 aaaagactaa gttcaaagaa gtacaaagta aaagggcttg tcgtacttcg acaagccctt
    13561 ttactgtagg ggcgccacgc ggggcgccca tatctgttag ttgtcagtgg ctagccatca
    13621 gcttatagct aaccccaacc tctccggcct ttcaggccac ctcccctatc aggggaggca
    13681 aaactgcgaa ctgcggccgg cgggctcgtc acgtgcgagc ctctacttat tttatgacga
    13741 tgttgatgat tttcttcggt acgacgatga atttgactac tttcttatcg ccgatgaatt
    13801 cctggatgtg cggtaaagcc aggacttttt cctgcaaggc atctttgtcg atgtcgacgg
    13861 ctacggtcag gcggtcgcgt actttgccgt tgatctggac aggcagttcg atttcatcga
    13921 cgacgagagc cgatttgtcg acttccggcc aatcctgggc atggacgctg ccttcaaagc
    13981 cgcatttctg ccagagttct tccgtaatat gcggtgtgaa gggagccagg agcttgagca
    14041 ggttcttgtt ggcttccagg gcaacggcct tgtggatggt cttgttagcg ccgacatagc
    14101 catgcatggc gttaacatat tccatgatcg agctgacagc cgtattgagg ccgtagttgc
    14161 cgtctgtgcc gcggacgtct tctgtgacct tggcaatggc tttgaattcc tggaaacgca
    14221 gttctttttc ggcttccgta tagtccgtcc cttcaaggcc gtctttgacc aagtcctgga
    14281 actgatggac aatgcgccat acgcggttga ggaagcggta agagccttcg acgccctggt
    14341 cagaccaggc caggtcgcgt tcgggcggag ccgcgaagag gatgaacagg cgggccgtat
    14401 cggcgccgta tttggcgatg atttcttccg gcgatacgat gttgcccttg gacttactca
    14461 tcttgctgcc gtcgaggagg accatgccct gtgtcagcag gcgttcgaaa ggttcgacat
    14521 tgttgaccag gccggcgtca tagaagacct tcatgaagaa acgggaatac atgaggtgca
    14581 ggatggcgtg ttcgatgccg ccgatatact ggtcgacgtt catccaatgg ttggctttct
    14641 tcggatccca ggctttttca tcattgcggg catcggtata gcggagatag taccacgacg
    14701 agcagacgaa ggtatcgatg gtatccgttt cacgcgtagc cgggccgccg catttcgggc
    14761 acgtgcagtg gacgaagtgt tcgctcgtag ccagcggcga tacggcgcca gccgtgaact
    14821 tgacgtcttc cggcagcttg accggcagct ggtcttccgg tacaagctgt tcgccgcatt
    14881 tcgggcagta gatgataggg ataggagcgc cccaatagcg ctgacgggaa atgagccagt
    14941 cgcgcaggcg atagttgacg cggcgtttcc ccagtccttt ttcttccatg tagtcgatga
    15001 tggctttcat ggctttatgc atttccatgc ccgtgaaagg accggcattg accaggacgc
    15061 cgtccttgtc ttcataggca tggtccattt cttccagctt cagttcgtgg tctttcggct
    15121 gcaaagtgat gatcttcggg atgttgtatt tcgtagcgaa catccagtca cgctggtcac
    15181 ccgtcggtac gcccatgacg gcgccggtac cataatcgta gaggacgtag ttggtgatcc
    15241 aaatctggac gctcttgccc gtcagcggat ggacgcagtc gatgccggta tacatgccga
    15301 ctttttccga ttccgtgctc gtccgttcga tatcgctggt attgcgtact ttttcgcaga
    15361 atgcttccag ttcggctttc ttgggattgt tttccaaaat cttgtggacc agcggatgtt
    15421 ccggcggcag gacgacgaaa gtgacaccgt aaatcgtgtc aggacgcgtc gtgtagacgg
    15481 cgactttttc cttcaggctg ggaatgtcga agctgaattc caagccttcg ctgcggccga
    15541 tccagttctt ctgcatggtc ttgacgcgtt ccggccagcc cggcaggagt ttcagatcgt
    15601 cgagcaggcg gtctgcgtag tcggtgattt tgaagaacca ctgtttcaag tcctttttga
    15661 cgaccggtgt gtcgcagcgc cagcatttgc cttcgatgac ctgttcgttg gccaatacgg
    15721 tgttgcattt ttcgcaccag ttgactttag cttctttgcg gtaagccagg ccgcgtttgt
    15781 agagcagttc aaagatccac tgcgtccatt tatagtattt ttcgtggcac gtagcgactt
    15841 cgcggtccca gtcatacgac aggcccaggg ctttctgctg gcgt
//
LOCUS       DAWABZ010000034        15767 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_1014_length_15767_cov_2.949084, whole genome shotgun sequence.
ACCESSION   DAWABZ010000034 DAWABZ010000000
VERSION     DAWABZ010000034.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 15767)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15767)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15767
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1014_length_15767_cov_2.949084"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6836..6845
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3571 a         4291 c         4059 g         3836 t
ORIGIN      
        1 gtccgcgata ttgccgtatt atcgcatgat ttagtatcag gctgatgtgt tatattatct
       61 atggatatgg gccttttttc tatacagtag tgttttaata gttgagctaa tcaactattt
      121 gcaaaatcag ccttttctcg taaaaatagt tcaaaaaatt atttcttgcg aatgattttc
      181 tctggcagta taattacatt tatctatcaa tttattttta cggagggtta ttcgcgatgc
      241 agcgattttt atcttatgaa aatacgattt tagccgtcgt catgatggta tctttcatca
      301 atccttttac cagcagcgcc ctgacgacag ccctgcctag catcggtacg gcttatggtg
      361 cgactgatgc tcagctcagt tgggtcattg agttgttctt gatttcttcg accgtgacca
      421 tcatgccaat cagcaagatt gccgaccgga ttggcaagcg tcatatcttt ttattcggcg
      481 tctccttgtt ctgtgtctct tcgctggccg tttattttgt gaactccttg tacggtctgt
      541 tcttgctgcg catcctgcag gggctgggca gtgccagtat tttcgcgacc agcatggcca
      601 tcgtatccct ggtcacgccg ccacagcgcc gcggccgggc catgggcttt acgattgccg
      661 ccgtatattg cggcctgtcc tttgggcctg tcctgggcgg cttcttttct tattacttag
      721 gctggaagac gattttttat ttcatcgcct tcatctgtgc cctggcctgg ctgattgcag
      781 cccgcagcat gacgaaggaa gaatggatcg tcaacgcgaa aggcagtctc gatttgcctg
      841 gcagtctgtt gtatgccctg gccatgattg ccattatggt tggcttatcg gaagtaatga
      901 ctctggcata cgccaaatac ctcttagctg ccggtctcct cttgttccta gctttcttat
      961 acggtgaatg gcaccggaaa agcccggtcc tgccagtccg tatttttgca ggcaaccgcg
     1021 tcttctcttg ttccagtctg gcggccatgc tcaattactg cgcgaccttc ggcatcagtt
     1081 tcctcttgtc catttacttg cagcgcatta ccggcctcaa tgcccgcgat acggggctgg
     1141 ttttactgat tcagcccgta ctgatggccc tcttgtcccc tgtcaccggg gccctgtcgg
     1201 accgtatcaa tccagccgtc ctggcttctt ccggcatgat tttgattacc tttggcctct
     1261 gtctcttagc ggtggatgtc tcttaccctt ttttctggct catcgccctg gccctagtca
     1321 tcatcggcat cggctttgcc ctgttcacgg caccgaataa caatgctatc atgggggctg
     1381 tgacgccaaa atattacggc gctgcctcgt ctgtcgtcag tacggtccgc ctcatcggtc
     1441 aggtcctcag tgtggctatc atcaccctga ttctgtcgca aagcggggcc tcggctgcct
     1501 cggattggct ggcccagcac atccagcagg ccttcatcgt ctttacgatt ctctgtgcca
     1561 tcggcatcgt cccttcagct gcacggagta agtagtgatt agtaattcgt ggttcgtggt
     1621 tcgcaaaaaa gcgactgtcc tataaagaca gccgcttttt gagtattaag ttttgtagtt
     1681 tttagtgaat gattaaatgt aaagccatcg tctgcaaact acgaacttcg aactataaac
     1741 tatgtttttt attttacaaa ataatgagcc gagtcgtcga cgaggctgtt ttccaggagg
     1801 gctttgaatt ccggtgcgct catccccgtt gcctgggcgg agaactgata gtccccgact
     1861 ttccaatgag ccgcatagga cgtgtcactg atttttgcta tgctgacggt gacgccgtcg
     1921 atgacctggt ctttccaggt tacgctatag atgccagaaa tgtcttttat ggatttatcg
     1981 gctttctgcg gcatcgtgcg gacgcggacc gtcgtttccg gctgtcctaa gcgctggaag
     2041 ccgatatctg ccgtgttttt gccgatcaaa gaaagatagt agcagctgaa tccggcatcg
     2101 cgggtcaaat ataagggcgt aaagccggca tctttgacga cactttcata agtatcataa
     2161 gttacgatgg gattgggcag ccccatggcc tggaccttcg gcgtgctggc agccagggaa
     2221 acgctgccga tcatcaaggc cgcacaggct aatacagcgg ttctttttac agtatatttc
     2281 attttctaca cctcgttcac cataaaaaaa aattaaacgt ttaagtaact atgagtatac
     2341 ccaaacgttt aatttctgtc aaactaaaaa ctattcccac gttttccaga cttctggcac
     2401 gtagccgacg gtcgccttgg cgccattgcg gacgatgggc gtctttaaga cttgctggtt
     2461 cgccagcaca ttggcatctc tttcgtcatc gacggcatat tggatgcggg ccagcgtatc
     2521 ctgatccttg catttcggat tgagcatggc attgaggccg ccgacggcct gcttgacctt
     2581 ggtgtattcg cctttactca ggcctttttc tttcaagtcg atgaactggg ccttgatgtt
     2641 ccgttctttg aaatagcgga tggctttctt ggtatcaaag cactttttcg tgccgaaaat
     2701 ctggatattc atgggattcc tcctaatgag tagcggactg agggctgtgg accttctttt
     2761 ccgcggcttc tttttcttta ttagcttcga tttcagccca caacttgtcg gctgtcggtt
     2821 tccactgtac ggcataagcc ttgcatttat tacataagtg ttctgccgat tccggcgact
     2881 gcaggtctgt cgatttggca ccgctgcgtt tgaccatggc ggctaaatac tgcggatttt
     2941 ccagcatcgg acacgggcgc agatggttgt ggttgaaggg ctggcctttg gcatattctt
     3001 tgaagagcgg ctgctgcaag catgagagca ggctctgatt gttgatattg gcgctggaat
     3061 aatggatgaa gacgcacggt tcgacgtcgc cattggggtt gatatggcag taattgcggc
     3121 cgccagcgac acagccgtcg atgaactggc catcgttctg gaaatcgatg gcgaaaatcg
     3181 gcttgccacc ctttacgctg cggatttcgc ggacccggcg taccatatag gcccgctgtt
     3241 cttctgtcgg tagcaggtcc agcgaggcgt cgttgccgac gggcatataa tggaagtacc
     3301 acgtaaacca gacgcccttc ttgatgagca ggtccaggaa ttcgtcagac gtgacggtgt
     3361 cgatgttttt ccgggtataa cagatggacg tcccaaaaac gatgccgtgc ttgcgcagga
     3421 ggtccatggc gtgcatgacc ttgtcgaaat cgccttcgcc gcggcggctg tcattgactt
     3481 cacggaatcc ttcgatactg agggagaagc tgagattgcc tacgtccgtg acttcgtcgc
     3541 aaaaagcatc atcaatgagc gtaccgttgg taaaagcgtg gaattcacag tccgggtgtt
     3601 tccggcacag acgcaggata tctttcttgc gcatgagcgg ttcgccgccc gtgagtaggt
     3661 agaaatgaca gcccagagcc ttgccttcgc ggacgatttt atccatgacg tcgaacgaca
     3721 agttcagcgt atgaccgtat tcagcagccc agcagccgat gcagtgcagg ttgcaggcgc
     3781 tggtcgggtc gaagaggatg gcccagggaa tattgcagtg atatttcgcc ttggcggcgt
     3841 cgcgcgtacg caggccgtaa aatccagctt caaagcccag gtccatgacg gccgtcttga
     3901 cgacatgcgg gttcaaggta tccagagccg tattgaggaa gcggaaccat ttgccccctt
     3961 catcgatgag tctgcgtgca tcgtcaaagg attccttttc aaagaggtcg ccaaacagat
     4021 gctgcatgac ataagataag cgtttcaatt ttttttgacg gtctgccgga ttccgttcgg
     4081 ccatgatgcg gtcgaccagg atttcgacag ccttcttttc gatacgatgg gttaacttgc
     4141 tcataccaat catctcctac aaaattaaac aattgaaacg gcgctgtttc tatttctttg
     4201 tatagttcta ttatacgctt attttagcaa aagtcaaaaa gtcaatatga attggaaaac
     4261 aaaaggcttt tacttttttg atgatatact ttataaatta cttgtataat atttatttac
     4321 accataaaag ggaatttttt atggctaaga aagaaaataa gttcaagggg tatgacattt
     4381 cgacacaggc tgtacacact ggtgccggct atgacgcaga aacgggcgct gtccgccggc
     4441 cattgcacat ggccaacagc tacaagctgc ccgatgatct gtcgaaggtc aactattcat
     4501 cgacggattt gctgatgtat gcccgtaacg gcaacccgaa tcaacattgg ctggaagaaa
     4561 aagtagcggc ccttcatggt gccgatgacg ccatcgtcct gggaagcggc gtcgccgccc
     4621 ttcatgccct gttctggacc ctgctcaaga cgggcgaccg cgtcgtctat ccgaaagtca
     4681 gttatatggc agtctatcgc ctgttccacg aattattcaa ccagaaattc ggcgtcgaaa
     4741 cggtcatggt cgatatgact gacctggaag ccgtcaagaa agcgattacg ccggggacgc
     4801 gcctagtcca tattgaaacg ccggacaacc cgactgatgg catttcagat atcgaagcca
     4861 tcgctaaaat cgctcatgaa aacgaggctg tcttgtccgt cgacaatacc tttgcgtcgc
     4921 ctctcaacca gcgaccactg gacctgggag ccgatttcgt cgtcgaatcg ctgacgaaat
     4981 atatcaacgg ccatggcgat gcccagggcg gggccatcat ttccaatgat ttggcagcga
     5041 tggaccggat ccgctatgaa gcccaggtca acgtcggctc tgtcatcagc cccttcaatg
     5101 cctggcagat tttccgcggt gccgtgacct tccccttgcg gatggaacgc atcaacgcat
     5161 cgacgcagaa aatcgccgaa tggctggaac agcggcaaaa cgtcaccttc gtttcctatc
     5221 ccggcctggc cagccatccc ggtcatgaat tggccaagaa acagatgaag cacggctttg
     5281 gcggcgtcat ttcctttggc atcgcagccg acgacaaggc cgtcgaacgc ttctgcgcag
     5341 ctttgaaagt cgtcaccttc gccgtatccc tcggccatga cgaaagcctg attttcccgc
     5401 agcccagcta cgatgaacgc atcaatctct acccggaaaa attccgcaag ggcttcatcc
     5461 gtttcagcat cggcctggaa gacccggacg acatcatcgg tgacctggac caggtactga
     5521 aagccatcgg cttataaagt cagcggaacc agcggctgcc gtacaagaga atcgattggg
     5581 gaatccaccc cgacagaaaa cggagcaagg tcgagacgac atccggcgtg atgacgactc
     5641 ggcctttttt cacggcctgg aaggagcggc gggcaatggc ttccttcgtc ccggccaggg
     5701 gaaaatggcg gaacagccgg ccttcatcgg tctggcgggc cgtcgcgatg aatcctgtat
     5761 ctttcaccca atacggacaa acggcagata cgaggatacc ctgcggtgcc agttccacgg
     5821 ccagtgcccg gctgtagcgt aggagaaagg ctttcgtcgc cgcgtagaca gccagttccg
     5881 gaatgggcag gaaggctgcg caggaacaaa tctggaccag atgactgccg gcatgacaat
     5941 aaggcagtac agcctggacc aggctgacag cggcccggtc gttgaggtcg atcatgtttt
     6001 ccaggacgcc tgccggaacg gccgccacag gccccatctt gccaaagccg gcggcgtgga
     6061 tcaaataacg gaccgtgacc tggcaggccg ctaaggcttg ctgcagcgtc agcaaagaag
     6121 ccctgtccgt caggtccagg gcgtagatac ggcttttcgt cgtcagttct ccagccaggg
     6181 tttccaactg gggccggcgc cgggcgacga gccagatttc atccaatcct tcttggtcca
     6241 gcaatttcgc atattcccgg cccagcccgg acgaagcgcc ggtgacgatg gctatttcca
     6301 taagactccc tcctgacaag aacgatagca ctgcaagtat tcggcttcga cgtcggcaaa
     6361 gggacgcggc cggtaatcgg ggatacaatg gacctgtggc ggctcgccct gaggctggtc
     6421 gtgcaagaga caggtcaggt cataccagag gaaatcatcg tcaatccctt tgaggaggcg
     6481 tgtttcccct tccgtcaagg gctggcctaa gaatttctca taaatgatag ccagcaattt
     6541 ggcttccgtc tgccggtata tcggcatgag ctgcttcatc ggccggggca cgtcggacat
     6601 ataacactcc gtcgcatcgt gcagtaaggc ggccaggacc agccgggacg gcagctggcg
     6661 ggccatcact tcgttggcgc attgcagaca atgctggccg acggaataaa aagtcatcac
     6721 atggccgttg ccccgacaga tgagggacaa ggcgtgggca atatcttcga tgcagatgct
     6781 taccgggtcg ggatgggtga tatcgaaaaa ctttccgcta taggtagtga tttgannnnn
     6841 nnnnntgatt tgaggagaca tgatataagg ggttcttcct tctctatatt tttgattttt
     6901 gttatattat tccgataagt attgcttatc ggaaagttaa ggttttatgc tatatttgcg
     6961 gcatcttgcc gccgcgtttg ccgccatgat ttcacaaaat cgaatcgagc ttcttcatgg
     7021 cttcccgatt gcgtttatcc gtaatcgtat cgtaaatgtt cgccgtcgtg gcaatggtag
     7081 aatggcccag ccgcttggaa acgtagtcga gctcgatatt gttttccagc atcatcgtcg
     7141 catgggtgtg gcggaaggaa tggaaactgc cgccgccgaa ttcctttttg caccacatat
     7201 tgaagtaccg catgtcattg ctgttcatgg gacggccgtc ttcacgggta cagacgaaat
     7261 cgctttcgac gtagtcgtcg ccgtaaagac gatggttctt ggcctgctgg gccttatggc
     7321 ggagcagggc cttgcataag gtttcattaa aaggaatggt ccggaccgac gacttcgtct
     7381 tcggcgtggc cttgcagacg ggcacaccct ttttgtcata caaggtactg accacggaaa
     7441 gcgtgcggtt ttccatgtcg atatgggacc attgcagggc caggcattcg ccgacacgca
     7501 ggccagtaca ataggcaatc atgcacggcg cataatattt atgccctacc gggaaggcat
     7561 ggaaaataat ctgtatctgc tgatgtgaaa agatgaccgg ctttttccgg ggaatatcga
     7621 atttcggcat cttgacgttg gccatgggat tgatttcgat atactggcgg ttggagacgg
     7681 cccagcgcat ggcgtcgttg aagaccgtga aaatagattt aaccgttgac cgggaataac
     7741 catcctcttt caggctgttg atccagtcct ggatttctgc cgtcgtcagc gcttccaggg
     7801 cataattgcc aaaacgggca ccaatatgcc atttaatgat gccggaataa gaatcatacg
     7861 tattagggcc taaattgatg cgcacatcct tttccagcca gtcccgcaaa aaagccatga
     7921 ccttgacttc cggcttttcc ggcagctccc cgtcttcatc cagggctttc atggcctggc
     7981 gataggcttt cgccgcctgg gcatacgaag taccgccgac gcgttccaca cgctgccttc
     8041 cctgaccatc ttccgtcggg acttcaatcg tataatacca tttcttgcct cgttttcgta
     8101 aatacatatg cggcacctcc tttgtttact attgtactat gagaaaagac aagatgcaac
     8161 aaacgttcct gttgaatttg ccgatttaag gaaaataaaa caagtttccc atgtgattac
     8221 tcacaagatg ataaaaaaac gtctcataag gccatttatg aaaattaggc ggattttttt
     8281 gcaaataaag aaactatgtt cgacgaaacg attatccctt tagcgaacat agtttctatt
     8341 tgctttattg ggccgatatg gtacaataaa aatagagaat tagcaaattg ttggattcgg
     8401 tagaagcaaa gcacaatttt tatggaatat ttacgctgtt tatacattcc tttgttatac
     8461 tgaaaagact agcacagaaa aggaatgata tcatgaagaa actgacttat gaaaatattg
     8521 cccatgccaa agagctgaat gtttatttag aacagggaaa ccgggtcctc gatgctctgg
     8581 ggtatacgga ccattcgcaa aagcatacgg ctaaagtggc cgtcacggcc ggcaaaatcc
     8641 tgaagaagct cggctatgac gcccacatgg tggaacttgt gcgcattgcc ggttatatgc
     8701 acgatttggg gaacagcatc aaccgcgtag accatgccca tacgggggcc ctcatggcgt
     8761 tccatttcct gcgcgactgg ggcgcaccac cagaagatat tgctgccatc atctcggcca
     8821 ttggccagca cgatgaacgg agcggcatcg cctttgaccc cctgtctgcc gcccttatcc
     8881 tggccgacaa gagcgatgtc cgccgggacc gcgtccgcaa caccgtaccg gaaaccttcg
     8941 acaaacacga ccgcgtcaat tatgctgccc tgtcgtcgaa gctccacgtc gacacgcaca
     9001 agaaagtcat taccttgaac atcgagctcg atgaatcgat ttgctccatg attgattatt
     9061 ttgaaatctt ttcccagcgt atgctcatgt gcaagcgggc agccgaagtt ctgggcctgc
     9121 gcttcaaact tgtagccaat ggcaataaaa ttgcatgaca gcttctaact acatataatt
     9181 accataaaaa gtacatattt cgcagcttat cgaaaagaag gcgacaccta atttttaaca
     9241 tttaggcatg ttgccttttt tttccatgat acacttataa tatagtttaa aacatgttgt
     9301 agagaaagga atcatgcata tgctacgtta tggaaaagga acacttctca ctacggtgat
     9361 ggccggactg ctggccctgt ccctgctgac ggcaggctgc ggcagcacga caccgactaa
     9421 gagtgccgct gtgtcggtca aggccatgaa agtcctccag caggatacgt atgaatcgga
     9481 agtcctctca gccaaatcga atgtcgacaa ggcccggacg acgccaacaa ctccctcgtc
     9541 gacttgaacc gttatcagca gctggcagct tccggcgccg tagccgaaca gaccctgacc
     9601 aaccagcagg ccacggtgga cagcaatcag tcgagttacg acgatgccaa taccctcttg
     9661 cagaaggccc aggaaaactt agacgacacc atcatcaggg cgcctatttc cggcaagctc
     9721 tctgtggacg acgtacccag cgggacttat gtcacgccag gcaacacggc cctggtatcg
     9781 gtaggttcaa tcaatcctat ttacgtctag ttctcgatca atgaaaatga attcttgaac
     9841 ttccgcatgg ccaatgacaa tgagccggaa gaccctacgc cgccgacggc atccctgact
     9901 ttgagcaacg gtaagaaata cgatgaaacg tcgacatcgt accagttcga ccgggaagtg
     9961 tcggacagta ccggtacgat tacggtcaag gctctcttca ataatgccga tggcgtcctc
    10021 ctgccgggga tgttcgcccg tgttaccatg gaaggccacc ccttgaagga tgccatcctc
    10081 gtgcctcagc gtgcggtcca gcaggtcctg gaccagtcct tcgtcatcgt cgtcgggagc
    10141 gataataagt ccgtcgcccg cgtcgtcacc ttaggcgacc aggtcggcag ctactatatc
    10201 gtcaagagcg gcctcgataa ggacgatatc gtcgtcgtcg aaggcctgag taatctgaag
    10261 gaaggtcagg acctggacgt caccatgacc acggctgatg aattgggcct gtccctgacg
    10321 gcatccgatt cgtcggcttc gacttcttcc gtatcgctta cggctaaata aggaggcgcg
    10381 tcatgtctag tttcttcatc cgacggccta ttttcgccat cgtcatttcc atcatcatcg
    10441 tcatcctggg gggcctgtcc ctcctgtccc tgcctattgc ccagtatccg cagatttcgc
    10501 cgccgacggt caatctgagt acgacctaca tcggtgccaa tgcgaccatc gtcaatcaga
    10561 ccgtcgccca ggtcatcgaa gaccagctca aaggcgtcga tgatatgtcc tatatgtcct
    10621 cgacgtcgag caacgacggt tcgtacagcc tgtccgtcgt cttcagcctg gacagtgacg
    10681 gcaatacaga tgccatcaac gtccagaacg acgccaatct ggccaagaac agcctgccca
    10741 gcgacgtaca gacccagggc ctgaccgtcc ccacagcctc cggcgacatg gtcctcatgg
    10801 ctaacatcta ttcgccgaac gggacgtata accaggcgtt cctcaagaat tatgccgata
    10861 tttacctgct tgacaaaatc cagcgcatcg acggcgtcgg cagtgtcaat accttcggtt
    10921 ccagctatgc catgcgcgtc tggctcaatc tggaaaaact ggccgaacgc ggcctgaccg
    10981 tatcggacgt cattgcggcc atcaaggaac agaatatgtc ggctccggcc gggaccattg
    11041 gcagcctgcc ggcaccggcg acacaggaaa aacagtacag cgccaaagtc gaaggccagc
    11101 tgtcgacgcc ggaacaattc ggcaatatca tcttgaagtc tacctcgaat ggccagttcg
    11161 tctacctcaa agatgtggcc aatatccaga cggggacgca gaacgaaagc agcaactcca
    11221 aaatcaacgg caagacaggg attggcttcg gcatccagct ttccagcgac gccaacgccc
    11281 tggacacgat tacggaagtc aagaaggtcc tggaacaggc caaggaaacc ttcccgccgg
    11341 acctggatta taccattgtc atggataaca cgaatttcat caacgcatcc atcaacgaag
    11401 tcaaagatac ctttgtcgaa tcgctgctcc tggtcatcct cgtcgtcttc gtcttcctgc
    11461 agaaatggcg gacgacgctc atcccggtcc tgtccgtgcc ggtttctatc gtcgggacct
    11521 ttgcgtcctt taaggtcctc gatttcacca tcaacaccct gacccttttc gccatggtcc
    11581 tggccatcgg cctggtcgtc gacgacgcca tcgtcgtcat cgaaaacgtc gaagagcata
    11641 tgtccaagga taaactgtcg gccaaggacg cgacggaagc ggccatgaaa gaagtccaag
    11701 ggccgattat cgccacgact gctgtcttgg cctcgatttt cgtccctgtc gccttcatcg
    11761 gcggcgtcac gggcgtcctc tacaagcagt tcgctgtgac catcaccatt tccgtcgtca
    11821 tctcggcctt tcgtcgccct gaccttgacg ccggccttat gcggtgttct cctgaagccc
    11881 gatgataagg ccatccaggc cgggccgctg ggtgccttct tccgcgggtt caaccgggtt
    11941 ttcgatcatt tcaaggatcg ctacacgggg aaagtcggct ggatgatccg tcatctgaaa
    12001 tatgccgctg tcttcctcat cctggtcacg ggccttatgg gcatctgcta caaagttctg
    12061 ccgtcgacgt tcgtccccga cgaagaccag ggcgtgttta tggccagtat caccatgccc
    12121 gaagggagaa tcgattatcg ccaagttcaa taagactgtc gtccccaacc atcccgaagc
    12181 ccgcgtagcg gctttcaatc cggcatccct ccccggcatg ggccaggaag gcggctggac
    12241 catgcagctc atggataata cgggcctgtc cgatacggaa ctgggcaatg cggccaacca
    12301 ggtcgtcgcc gcctgctcgc agcggccgga attatccggt gtccgcacga gctatgccac
    12361 caatacaccg agcgtggcct atacggtaga ccgtgaaaaa atcaagaatc tgggcatcga
    12421 attatccgac gtcttcacgg cgctccagac caatttcggc ggttcgtcgg tcaatgactt
    12481 cacccagttc ggccggtcgt acaaggtcat cgtccaggcc gacacgtcgt atcgctctca
    12541 ggcagacagt ctgaatttca tttccgtcaa gtcgtcgtct gggaaaatgg ttcccctcga
    12601 tacgctgctg accagctcca tgaccaccgg cccgtcgtcg atcacccgtt tcaacggcgt
    12661 ccgcagcgtc accatccagg gcagcgccgc cagcggctac agttccggtg aagccatggc
    12721 agcggccgaa gaagcggcca agagcgtcat gccgacgggg atgaccatcg aatggtccgg
    12781 ccagagccgc gaagaaagca cgtcgtccag ttcgacgacg aaaatcctgc tcatggccct
    12841 ggtcttcgcc ttcctctgcc tggctgccct ctatgaaagc tggtccatgc cgttcgccgt
    12901 cctgttcacc gtaccgacgg ggattttcgg ggctgtcggc tcggaataca tcatccgcca
    12961 gctggcgacc ttgttcaacg ccaatgccag cggcttcttg aacagcgtct acatgcagat
    13021 tggcgtcatc atgatcatcg gcctggcggc caagaacgcc atcctcatcg tcgaattcgc
    13081 caaagagcgt gtcgacaagg gtatcgaccc gatggaagct gtcgtcatcg cctccaagct
    13141 tcgcctgcgg ccaatcctca tgacggcctt tacggccatc atcggctgcc tgcctctggc
    13201 ctttgccagc ggtgccggcg ccggagcccg ctgcggcatg ggcatcgccg tcgtcggcgg
    13261 catgaccttt gccaccctct gcggcctgct cctcatcctg gcgttctaca tcatttccga
    13321 aaaaatggcc cgcagcttct ggaaagccac aggcctgaaa cgaatcaaat tcaaacaccg
    13381 catgttgagg cggtgaaaaa aaagcttgtc gcatgtgcgg caagcttttt taagtggtta
    13441 gtagctagtg gttagcggat ggcagaaacc ttcacgcgac gccatcccta ctcatgtacg
    13501 atgagttcca ggatgtctgg gcgggtgtag tggccggcgg cgtcgaattc catgtggcgc
    13561 aggacgactt ggtccatgtc caggtcggcg tagatgatgg cttcccggtc ccagacgggc
    13621 tcggtgacgt agtgaccgca cggatcgatg atgcagctgc cgccgcggca ggccgtgtcg
    13681 ttcagtttgg cgacggcccc gctttcgacc aggtcggccg ggtacatgtc cttagtgaaa
    13741 tactggtcga cgttgaagac gtagcagcgc ccttcgatgg cgatgtgctt gaccgtatcc
    13801 tgccattccg ggttgtcatt ggtattcggt gccaaataga ggctgacgcc tttctggtag
    13861 agcgccgtcc gggccagggg catataattt tcccagcaga tgagcgagcc catgggcccc
    13921 cacgacgtct ggaccatggg gaaatacgtc gacgggtcat gagaatcagc ccagatatag
    13981 cgttctgccc cagtcggttt gattttccga tgatgagccg ccagcgtgcc gtccggtgcc
    14041 aagatgacat tgctgcaata cagggaatac gtcgtttcat cccgttcggt aatacccagg
    14101 ctgacataag ccccggcggc tttggccgca gcggctacgt cctgcaaatc ctgacggacg
    14161 acgacggcct ggtcataata gcgcttccag tcggcccgtt cggcttcgga acggctgccg
    14221 acggtaaagc cgaaggtcag gccataggga tagcatggaa ccagtgattc gggaaagacg
    14281 atgagctgtg ccccattccg gccggcctcc tcgatttgat ggacgacttt ctgcagcgtc
    14341 gcctgtttat cgaagaatac aggcgcagcc tgcacgatag cggcgcgtag tttcttttct
    14401 aaatttcgca tagcgaatcc ccctcatttc ttttttctta gtataccgta atttaaaaaa
    14461 atgtgctata ctgattcagt ccacagttta cggaccgata aggataacga ctgtgcagtt
    14521 ttcgggctgc cgtcgttatc cttttttgta tgataaagga gggcttatgt atttgctcat
    14581 cgataattac gattctttta cgtataattt ggccgattta gtggcggaaa cgggacgcga
    14641 cgtacaggtc tgccgctgcg accagattgg gcggaatgag attgccgctt tgaaaccgca
    14701 gggactgatt atttcgcctg ggccgaaacg tctctcaaag cctatttgaa aaagcattac
    14761 caggccaatc agaccgccct gcccttgaca gctggggcca tcggctatgt cagctatgac
    14821 tacggcctgg cactccatgg gatgaaatcc cgccacgccc ctgcctttcc cctgcctgat
    14881 atgaagtggg tcttttacga tttcttcatc atcgaacacc ggcaaagcca cgccgtatgg
    14941 tttgctgcca atggccagac gatgccggcc gaagccctgc tagcacagat ggaagagaaa
    15001 atccggactc gccttcagga aaaaacgctc ccgcatactg acgaaaatag ccaatatgcc
    15061 ttaaccgtcc aagccgacag cacgacggaa acctatggaa aggctttaca aaaactccgg
    15121 gactacctct tcgccggcga tgtctatgtg acgaatttca ccagcacctt aaccgtccag
    15181 agccactgcc cgccgtacac cttcttttcc cgtctgcgcc gagacaatcc gtcccctttc
    15241 ggggcttatt tacaatatgg ctcttatcaa atcatcagtg cttccatgga acggctgctg
    15301 tgcctgcgcc atgaccagat tgaaacgcgt cccatcaagg gcacacgacg ccgcggcaag
    15361 acgcctgctg aagatgaaaa gctgcggcag gaactggctc aatcgcaaaa agaccagagt
    15421 gaattactga tgatcgtcga tttggagcgc aatgacctgc acaaaatctg ccagcctggc
    15481 agcgtttcgg ttccgacgtt gtttgtcatc gagccatatg ccacggtttt ccatttagtc
    15541 gccgacgtaa cagggaccct gcggccgggg cagacagcca tcgatgcctt gatggccctc
    15601 tttccaggcg gctccattac gggtgcgccg aagcggcggg ctatggaaat catcgacgaa
    15661 ttagaattca gccggcgcca gctttatacc ggaacaattg gctacctttc tttagatggg
    15721 aactgtgact taaacattgt catacggacg gccgtccact acgatga
//
LOCUS       DAWABZ010000035        15440 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_1049_length_15440_cov_3.084823, whole genome shotgun sequence.
ACCESSION   DAWABZ010000035 DAWABZ010000000
VERSION     DAWABZ010000035.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 15440)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15440)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15440
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1049_length_15440_cov_3.084823"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3447 a         4374 c         4330 g         3289 t
ORIGIN      
        1 ggagatgcct tcggagatgc tgtagcagaa gggcatcgcg atgatggtga tgtatgccgg
       61 gatggattcg ctgaagtcgt cgaagtcgat gccggcgacg gacgtcagca tgaggaagcc
      121 gacgatgacc agggccggtg ccgtggcaaa ggccgggatg gccatgaaga acggcgacag
      181 gaagagggac agggcgaaga ggacagcgac gcagactgcc gtcaggcccg tgcggccgcc
      241 ttcgctgacg ccggtagcgc tttcgacgaa ggtcgtcgtc gtactggtgc cgatgacagc
      301 gccaactgtc gtggcgacag cgtcggccag gagggcgcct ttgatacgcg gcaggcggcc
      361 ccgttcatcg agcatattgg ctttggacga gacgccgatg agtgtgccca gggtatcgaa
      421 aatatcgacg aagaggaagg cgaacatgac gaccaggaag tccaggctaa agacgcggct
      481 gaagtcgagc tggccgaaga tggggctcag gctggccggg gcaaaggcag cggcgccgcc
      541 ggacaggtcg gggataacgc tgaacatctt tatttccgga ttgggcacgt agaggccggt
      601 caattcgcaa agaatggcca ggatccaggt aaagaggatg ccccagagga tgttgccgcg
      661 gactttcttg accatcagga cggctgtgaa gacgatgccg atcatggcca ggagaaccgt
      721 aatgccgaca gtgtggaagg tacccgtagc ggcagactgt ttgaacgaga acagggcaat
      781 cttggttgcc gggttggcga cgatgatctg ggcgttcagc aggccgatga gggcgatgaa
      841 caggccgatg ccggcagaaa cggctttctt caaggtcatg gggatggcgt tgaagatagc
      901 ttcgcggaca ttggtcaaag acaggatgat gaagataacg ccttcaaaga agacggcggc
      961 cagggccatt tcccaggtgt agcccatctg caggacgacc gtaaaggcga agaaggcgtt
     1021 caggcccatg cccggtgcca gggcgaaggg atagttggcg aaaaaggcca tgcacagcgt
     1081 cccgataagg gaagccaggg cggttgccgt gaggacggca cctttgtcca tgccggcggc
     1141 gctcatgatg gtcgggttga cggccaggat gtaggccatg gtcatgaagg tcgtgatacc
     1201 agccaggatt tccgtgcgga ccgtcgtgcc attttcgctc aagtggaaga aacggtcgag
     1261 gaaggacggt tggttttcca taatacattc acactccttt agtaataaaa caagttgaca
     1321 tccatccgga aatacatgaa gccaaatagc agacgacgga catcaactga tatcattatt
     1381 atagggtgtt ctgccttttt gggcaactat tttgtcaaaa aaaggaaatc tatggtacaa
     1441 tgagtccgaa agataaaaat cttacgcctt taccgatagg aaggagtaca ttatgactat
     1501 gacacggaat cttttgaaac ggcgtattgc cgtagcccgg ggcttggaac cgggggacgt
     1561 cgtccttaaa aactgccaga tcgtcgacgt ctatacacag accatccggc ctggggatat
     1621 cgtcatcgcc gacggcatca tcgccggcgt cggcggcccg tatgaaggac aggaagtcat
     1681 cgatgcccat ggggcctatg ccgcaccggg cctcattgaa agccatatcc atatcgaatc
     1741 gtcctatgtc agcccggaag agttcggccg cctggtcgtc cccctgggaa cgacgacgat
     1801 cgtcgccgac ccgcatgaaa tcgtcaacgt ctgcggcctg gccggccttc gttacatgat
     1861 ggaagccgct gaaaagacgg ccctggacgt ccgtttcatg gtgccgtcgt gtgtcccggc
     1921 gacgcctttc gaccacagcg gggccgtgct ggaagcagac gacatgaaat cgcccctggc
     1981 cgacgaccgc gttctcggtg tcggtgaatt catgaactgc ccgggcatcc tgaacggcga
     2041 tgatgccgtc ctggacaagc tcgtcgccgc ctatgaggcg aaaaagccca tcgacggcca
     2101 cagccccggc gtcactggca aggacctgca ggcctatgcg tcggcccaga tccggacgga
     2161 ccacgaatgt ttcaccttgg acgacgtcga agcgcgcctc caggccggca tgtacgtcat
     2221 gctgcgcaac ggttcggcct gccatgacct gccaaatctg gtccgggccg tgacgccggc
     2281 caatctgcgc cgcttcctgc tctgttcgga tgacctccat cccaagacga tctttgagaa
     2341 aggccacctc aacgaacacc tgcgcctctg cgtcgccgcc ggcctgtcgc cgcaagcggc
     2401 tatcaccatg gcgaccctca acgccgccga ctgctaccac ttcgacgacc gcggcgcctt
     2461 ggcaccgggc aagcgggcgg acatcgtcct ctttgaagac ctgaaggact tccacgttct
     2521 ggcgaccttc atcggcggtc agaaaacagc ggaaaagggc cagtatctcc tgccctttac
     2581 gcgggctgat tacagcgccg tcggcagctc tgtctgcatc gccggtttct ccgaagagaa
     2641 aatggttatg cacttgacgt cggaccgggt ccggactatc gacatcgtgc ccggcggcgt
     2701 cgtcacggaa aaaggggaag ccctcatcca tcgcgatggc cgcggcgatt tcgtctacga
     2761 cccgcaggcc gatatcgtca aagttgccgt cgtcgaacgc catcacggca cgggctgcgt
     2821 cggcctgggc ctgctgcgcg gttatggcct taaacgaggt gccgtcgccg tatccgtggc
     2881 ccacgattca cacaacatca tcgtcgccgg cacgaacaat agggatatgg ccgctgccgt
     2941 cgaggccctg gagggccaga agggcggcat ggccatcgtc ttggacggaa cggtccttgc
     3001 atccatcccg ctgcccattg ccggcctcat gagcgaccag ccggccgccg ctgtcgatgc
     3061 agctatggac gagctctacg ccaaggccca ccatatcctg ggtgtcagcg ctgacgtcga
     3121 tgtcatcatg acgttgtgct tcatgagcct gcccgtcatc cccaagctga aactgctgga
     3181 cacgggtctc ttcgacgtcg aagccttcga tttcgttccc attgaacctt aaggaggtat
     3241 gattatggat gccaagaaag ttaagttagt cgtcatcaag tctcattgtg atctttataa
     3301 agaaggcgat gaaatcgtct tcgacgggcc tgtcatcgat aaagcaaaga gcggcgccct
     3361 ctgcatgacg gccctccagg ccctttatcc ttatgtcttc gccggccgcc agggttcgct
     3421 ctggccgtcg gatatccagt gtcccgactg cgacgaaggc gtcatcttcc gcgtggtgga
     3481 acaatgatgc ataaccctaa tagggcagaa cgagaaggta ttggcaaatg gcttgacgac
     3541 cgtgatataa tgaaaggtag aatgatagtc gtgtgagtgg ggaggaatgt gaaagttgat
     3601 actcagcaaa gcctgtaaga caaagatggc tgctcttttc atcggcctca tggccttgat
     3661 gccggtgcag gccattacgc aggaagaaat ggtctatgac caggtcagca aatatatccg
     3721 cggcagcgaa gcggcggcca ttgccaagga tatctgtgat gcatcctata cgtatgacgt
     3781 cgacccgatc ctcgtcgccg ccgtctttac gacagaaagc catttcgata attctgccgt
     3841 gtccggcgtc ggtgccgtcg gcattgccca gcttatgccg tctacagcgg catcactcaa
     3901 ggtcaatccc tacaaccgca gggagaacat ctttggcggc gtcaagtacc tcggccagat
     3961 ggtcgaacgc taccgcacat gggaccggcc cttcgtctat gccgaagcgg catataatgc
     4021 cggaccgggc gccgtcgacc gggcccgagg cgtgccgcat tatacggaga ccatgaatta
     4081 tgtccagacc gtcgaacaga cgcggcggga aatctggcgc cttgccggtc atgaagccat
     4141 ggccctgaag ccctacaagc ggtcggccat gaccgtgacg gaaacggaag cacccatttt
     4201 tccgagcctg aaagaatggc atgaacagca cagtcagaaa gcagccgctg tccagactaa
     4261 gactacggtc cggcccaaga cggcccgcgt agccaattcg gtccgcagcc cgcagcctgt
     4321 gtcgtattgg acgacgtcgc ggccctggga cacgcggagc agtaaaaaat aatatcgagg
     4381 aagggacctt gcatcagcga ttatgcgatc tccctttttt tacggcctgg agccgtaagg
     4441 aatgaaaaaa ggagttgtga aatggaaaaa gaattgattc acctggagca cgtatccaag
     4501 acctatgatg ggcacaaggt attagatggc cttgatctga cgatttctga aaatgaattc
     4561 gtgactctcc tcggccccag cggctgcggc aagacgacga cgctccgcct cattgccggt
     4621 tttgaaacgc ctgatgaagg gcgtatcctc ttcgatgggg ccgatatcac ccagctgcct
     4681 ccgaaccggc gtcaggtcaa tacggtgttc cagaattatt ctcttttttc ccatatggac
     4741 gtggccgaaa acatcgcctt cgggccgaag attcacggca agtcccgcga ttatatccgc
     4801 gataagatcc gttatgccct gaaactggtc aacctcgacg gctacgaaca cgccaacatc
     4861 gtcgacttgt ccggcggcca gcagcagcgc gtggccatcg cccgggccgt cgtcaatgag
     4921 ccgcgggtcc tgctcctgga cgaacccctg agtgccctag accggaaatt gcgccgcagc
     4981 atgcagctgg aactcatcaa gatcaaagaa gaactgggca ttaccttcgt cttcgtcact
     5041 catgaccagg aagaagccct gagcatgtcc gacacggtcg tcgtcatgaa ccagggttat
     5101 atccagcaga tcggcacgcc cgaaagcatt tacaatgaac cggaaaacgc cttcgtcgcc
     5161 gacttcatcg gtgacagcaa catcgtcgac ggcctcatgg tacacgacaa actcgtgcgc
     5221 atcgccgact acgacttcga ctgtgtcgac gtcggcttcg ggacccaccg ccctgtcgac
     5281 gtccatatcc ggccggaaga tatggaactg cagccgcctc gcgacggcgg tttcaacggc
     5341 gtcgtcacca agacgatttt tgaaggcatg acctataacc tcactatcca ggcgcggggg
     5401 atcgagtggc tggtcaaatc gacgaagcac tatcagcccg gcgacgaagt cagcctctgg
     5461 gtggacccct tcaatatcca gatcatgaat aaaccggaat cggaagacga aagggcgatg
     5521 gaaaatgatt aattcagcag cgattcgcaa cctctttgcc gcgcccttcc tcatctgggc
     5581 ggtcttattt atcttctgtc ccgtcgtggc catcgcctgg tacggcctga ccgatgccca
     5641 cggcgccttt accctggcca atatcagcct catcgcccac tgggagtacc tgaaggccct
     5701 ggaactgtcc atcgtcctgg ccctcatcag cacggtcatc tgcctggtcg tggcttatcc
     5761 gctctgcctt atcctgacgg aaaagaagcg ggggaagggc tcgctcctgt tcctgctctt
     5821 cgtcctgccc atgtggatga actccctgct gacgaccatg gcctggcaga cgattctgga
     5881 aaagaacggc atcctcaacc agttcctgcg cctcttgtcc ctgcctgacg taagcctcat
     5941 caacacgccg gcggccatcg tcatcggcat gatttacaac ttccttccct atatgatcct
     6001 gcccctctac gtcgctctga gcaagatcaa cggcaacgtc atcgccgcgg cccgcgactt
     6061 aggggccaat tcctggcaga ccttcaccaa ggtcatcctg cccttgtccc tgcccggcgc
     6121 catcagcggc atcaccatgg tcttcatccc gtcgctgacg acgttcttca tcagcggcct
     6181 cttagggggc aataagatcc tgctcatcgg caacatcgtc gaacaggaat tcaccatggc
     6241 ctatgactgg catctgggca gcggcctgtc tctggtgctc atggtcttca tcatcgtcaa
     6301 catggtcgtg acggcttatt tcgaccgcag cgaagggcag gtgaagaagt gatggaaaaa
     6361 gtgaagaaac tctacgtcgc cctggtcgtc ctcttcctct acgcgcccat cggcgtcctc
     6421 gtggcccagt ccttcaacgc cagccgttac cgcggccact ggacaggctt taccctgcaa
     6481 tggtacggcg agttattcca gaatgaaggc atctttgacg ccttgcaaaa taccattacc
     6541 atcggtctct cgtcggcggc catcgccacc gtcctgggtc ttctcacctg catggccctg
     6601 cgcggcatgg gtcgcaaaca gcggtcggtc tatctcggcg tggctagtat gccgctgctc
     6661 aatgcggaca tcgtcaccgg gatttccctg atgctcctct ttttaggcat gggcctgcgc
     6721 ctgggctacg gttcgattct catggcccac gtcgtcttca acctgccgta cgtcatcttg
     6781 tgcatcatgc cccagttcct gtcgctggac cgcagctgtt atgatgccgc ccgcgacctg
     6841 ggcgcgacgc cgttcgtcgc attccgccgc gtcgtcctgc cggaactgcg gcccagtctc
     6901 ctggcaggct tcatgctggc cttcaccatg tcggccgacg acttcatcat tacgcacttc
     6961 accaaagggg ccggcatcga tacgctgccg acggaaatct atgccgaatt gaaattgggc
     7021 atccatccgg aaatgtatgc cctttcgacg ctcgtattcg tctttgtcct ggtcttcgcc
     7081 gtcctgctca tcgtcaaccg ccggcgcctg gccttgggtc ggaactggta ggaaagggga
     7141 aacgctatga aatatatccg catgattctc ctcggtctct gctgtatcct ggccctgagc
     7201 ggctgtggct tctttgacag cgaagaagac ggggacaacg tggtctatgt ctataactgg
     7261 ggcgattacg tcgacccgga tacgctcaag cagttcgaag aagaaacggg catccgcgtc
     7321 gtcatggatg aattcgatac caacgagagc atgtatcccc tggtcgcggc cggcgccgaa
     7381 cgctatgacg tcatctgccc gtcggactac atgatccaga agatgatcca caatgatctt
     7441 ttgcagcctc tcgattattc ccagctgccc aacgcccgcc agtacgtagg caaacagttc
     7501 tggacccagt ccgagtcctt tgacccgggc aaccgctatg ccgtccctta ttgctggggc
     7561 accgtcggca tcgtctacaa tcggaccatg gtcgatgaac ctgtcgacag ctggtccatc
     7621 ctttggaacg agaaatataa gaatgaaatc atcatgcagg actcgccgcg cgattccttc
     7681 atggtcgcct tgaagcgtct gggctattcc ctgaacacga cggatcctca gcagatcaaa
     7741 gaagcgcggg atatgctcat cgcccagaaa cccctggtcc aggcctatgg catcgatgac
     7801 gtcaaggaca agctcgtctc cggcgaagcg gccatgggcg tcgtcttttc cggtgaagcc
     7861 ctgcgcatga tcctggaaaa caggaacctg gaatacgtca tccccaaaga agggaccaac
     7921 ctctggattg actcgtgggt catccccaag aatgccgaac acgtcgaaaa tgcccataaa
     7981 ttcatcgact tcatgagccg tcccgacatc gccctgaaga acttcgagta cctgacctat
     8041 tcgacgccga atacggccgt cccggccatg gaagaagacg aagactataa agataacccc
     8101 gtcgtcttcc cgccggaaga agcttatgcc ggccaggaaa cgtacaaata cctgggcgaa
     8161 gaaggggaca agcgctataa cgacgaatgg atccaggtca aggtctggtg attttttagg
     8221 ataaatgctt ttcaagaata caaatgtgta gtataatgag tacaagttca gaatgagacc
     8281 aaagaaggaa ccgttgcacg aaggcttttg ccatcatgca cggttccttt tgctttatag
     8341 tttgtagtat ggagtttgta gaaaatggct tcaaatttaa aaacacccac taaaaactac
     8401 aaactacaaa cttcgaactt ctgttgtatg agattgggag gcggggatat ggctgagaaa
     8461 aggcctttgg ttttcaatgt gcagaaattt tccgtccacg acgggccggg gatacgaacg
     8521 acgattttct tcaaaggctg tcccttggcc tgcccatggt gccataatcc ggagagtcag
     8581 cgcttcgagg ttgagagaat ggagcgtagc gatgggggac aggatgaggt cgggacctat
     8641 tattccgtag cagaactcgt ccgccaggcc gtgcaggacc agattttcta tgaacgttct
     8701 ggcggcggcg tcaccttgtc gggcggtgaa gtcctggccc agcccatggg gtatatcgcc
     8761 gagctcgtcc gggccctggc tgaaaaaggc atttccgtcg gcatcgacac cagcggcgct
     8821 gttcccttta cggcttttga accggtcctg ccttatacgg acttcttcct ttacgacatg
     8881 aagctctttg acgccgaacg ccaccgacaa tatatgggcc agggtaatag gcagatactg
     8941 gagaacatga agcgcctcag cgacgccggc gccggcctct atctgcggct catcatggtc
     9001 gacggcgtca ataccgacgg ggaccgggat atccggccct tccaggactg gctccaggcg
     9061 cagcagatac gcgtggaaag aatccatctc ctgccgtacc atgagctggg acgccataaa
     9121 tatgaggccc tgcatcggac gttccagccg ttccatgccc cgtcagggca ggccttggaa
     9181 gccatccggc aatcttttgc tgtactcggt tatgataccg tcatcggcgg ttagatagga
     9241 gggaattcac atggcaacag ctttgaaaga acagaaattc gtggaaggta ttcggggatg
     9301 tacggaccgt atccgccagc tgcgggaaga aagcgttcac ggcgccgtcc cgacgatttc
     9361 catggaacgg gccgtgctgc tgaccgaagc gtaccagcag tacgaaggga cttgttccat
     9421 tcccgtcctg cggggcaaag ccttttacta tctcatggcc catcggaccc tctatctggg
     9481 acggggctcg ctcatcgtcg gcgaaaaggg gccgaaaccc tgggcggcac cgacctttcc
     9541 ggaactgtgc tgccataccc tggaagactt cgacaccctg aacagccgcg aacgggtctt
     9601 cttcagagtg accgacgaag ataagcgcat ccagcgggac gtcatcatcc catactggaa
     9661 ggaccgggcc atgctgacca agatgaacgg cctgctgccc cgggcatggc agaaattatt
     9721 tgctgccggt atgtttacgg aattcctgca gcagcgcggt ccgggccata ccgtggccga
     9781 tggcaagata tactggaagg gctatggcga tttcctgcgg gacatccagg aacgcctggc
     9841 cagcctcgat ttcaataacg atatggatgc cctggaaaaa cgggacgaat gggaaggcat
     9901 ggcctgggtc tgccggggca tgatcctctt agggaaacgc tatgccgccc tggcccggga
     9961 catggcggcg aaagaaaaag acccggcctg gcgccgagac ctgctggata tcgccgccgt
    10021 ctgcgacgtc gtcccggaac accggccaca gacgttccgc caggccatcc agatgtactg
    10081 gttcacccat atcggcgtga cgacggaaat gaacaactgg gatgcctatt cgccaggcaa
    10141 gttcgaccag tatctggaac ctttctatga ccgggacctg gccgaagggc gcctgacccg
    10201 ggacgatgcc aaggagctcc tggaagcgct gtggatccag ttcaataacc agccggcacc
    10261 gccgaaagtg ggtattaccc tcaaggaaag cgcgacgtat accgatttct gcaacatcaa
    10321 taccggcgcc ctgcggtccg acgggacctg tggcgtcagc gatgtaagct atctcatcct
    10381 ggaagtcatg gacgacatgc gcctcctcca gccgagctcc aacgttcaga tttcccagaa
    10441 gacgccggaa cgcttcctgc ggaaggctct ggaaatctcc cgcaagggct ggggccagcc
    10501 ggcgttctat aacagcgaag ccatcgtcca ggaactgatg tatctgggca aatccctcga
    10561 tgatgcccgg gcctgcggca ttgccagcgg ctgcgtcgaa gccgggacgg ccgggaaaga
    10621 agcctatgtc ctgacgggct atctcaatat ccccaaggtc ctggaactgg ccctgcatcg
    10681 cggctatgat gcctataccg gggaaaaggc aggtatcgac gtcggcgacc cgttgcagtt
    10741 tgcgtcatat gacgatgtat ggcaggcttt cctgaaagaa ctgaaatacg tcgtcgatgt
    10801 caaagtggtc ggcaataacc tcatcgaacg gctctatatg cagtacatgc ccgtgccgct
    10861 gctgtcagtc atcaccgacg actgcattgc caatgggaag gactacaatg ccggcggcgc
    10921 ccgctataac acgagttata tccagtgtgt cggcattgcg accattacag attccctggc
    10981 cgtgctgaaa cagcaggtct ttgaacagcg gcggttcacc atggaaggca tcatcaccgc
    11041 ctgcgaccgg gacttcgacg gcgacgatgc cttgctcgac gtcgtcctcc atcatacgcc
    11101 gaagtacggc aacgatgatg actacgccga cgctatcctc cacgacgtcg accgggctct
    11161 gcaagcaact attgccggca ggccgacgcc gaaaggctcg aagacggtcg tcgaattcct
    11221 gccgacgacg tgtcacgtct actttggcca ggtcatgggc gccagcccta acggccgccg
    11281 ggcccatgtg cccctgcccg atggtatttc gccggaaaag ggcgctgata cccacggacc
    11341 gacggcggtc atcaagtcct gtgccaaact ggaacagctc aagactggcg gcgctctgct
    11401 caatcagaaa ttcacgccgt ccgtcgtcgc cggcccgaaa ggcctggctt gcctggctgc
    11461 cctggtccgg tcgtacttca ccatggacgg ccatcacatc cagttcaacg tcgtcgacag
    11521 ggaaaccctc ctcgatgccc agcgccaccc ggaacaatac gaaaacctca tcgtccgcgt
    11581 cgccggctac agcgattact tcaacaacct ggacaaagcc ctgcaggacg aaatcatcaa
    11641 ccgcactgaa cagagctttg gggcgtaaaa aagaccgtgc ataatggttt ctgccatcat
    11701 gcacggtttc ttttttttgt tttaaattgt tgtaaatttt tttcattgta ggggctcgca
    11761 cgtggcgacc cctacagtgc caaaagcgag ttcacaagat tcgcggcggg ccttagagcc
    11821 acgagccacg aactacttgt cgaaggcttt cccagtgagc atttcgtagg cttctttgta
    11881 tttggcaatg gtcttgtcga tgacgtcctg gggcaggagg tagtcgctgt ccgggttggc
    11941 tttgagccag tcacggacga actgtttgtc atacgacggc tggctctggc cggctttgta
    12001 gccttcgagg ggccagaagc gggagctgtc cggcgtgagc atttcgtcgc ccaagacgat
    12061 attgcctttg tcatcgaggc cgaattcaaa cttcgtatcg gcaatgatga tgcctttgga
    12121 gagggcgtat tcagcacatt ttttatacag ggccaaggtg taatcgcgga ttttttcagc
    12181 gtattccttg ccttttcccg ggaaggtctt gtcgatgaaa gcagcgcctt cttcgagaga
    12241 gacgttttca tcatggtcgc cgagttcggc tttcgtgctc ggtgtgaaga tcggttccgg
    12301 gagtttttct gattctttga gacctgccgg cagtttgatg ccgcaaacgg tgccattctt
    12361 ctgatagctt tcccagccgc tgccagtgat gtagccacgg acgatacatt ccatgggaat
    12421 catagtcagt ttctggcatt tcatgctgtt gccgatgaac tgttcctgct ggaagaattc
    12481 cggcatgtca gcgttgtcga cggaaatcat gtggttcggg atgatatccg acgtcatatc
    12541 gaaccaaaac ttggacatct gcgtgaggat ggcgcccttt ttggtgattt tgttcttgag
    12601 gatgtggtcg aaagcggaga tgcggtccgt tgcgaccatg ataatgctgt cacctgcgtc
    12661 atagacttcg cgtactttac cggatttaaa aggggtgtat tctttcatct tgtctggcct
    12721 ccatgattca gaacttattt tattatggta gaacagtatt atcatagcat attttgggga
    12781 aagaaaaaag gttttgtcat caaaggacaa aacctttttt tcgtggctcg taggggacgc
    12841 ccagagggcg tcccgcaaat catgtgtgat gtgtatataa taactcttct ggacgggcgc
    12901 cccacgtggt cgcccctaca atgcggtttg tgaggccacg tggtcgtgct tacgatacgg
    12961 tcgcggtccg gcttactgca gataataagt caggctgacg ctttggctga gggtgttggt
    13021 gccggcttcg actggtgtcg agtcggcttc catcttgttg acggcccgca ggctcatggc
    13081 agcgccgtag acgcggctgt acgacgggct cgtaccggaa atgttgattt ccttgacggc
    13141 accgagctgg ctgccagcgg cttcagctgc ggcctgggca gcgcgctgac cgttgtggac
    13201 ggcttctttg atcaggtttt ccttgatctg gtcggcgtgt tcattggtaa aacggacgtt
    13261 gttgatctgg ttggcaccga ggctgccggc cttgttgatg acctgcgata ccatagtgag
    13321 gtcggtcagt ttgacttcca ggctgttggt caccgtatac gacgtgactt tctggccctt
    13381 catgtcatag ttcggtgaca tatagaagcc cgtggtcttc atgttttccg ctgggatacc
    13441 catggccttc aaggcattcg tgacctggtt catggccaga ttattcttgg tccgggcctg
    13501 ctgggcatcg gcgtcggtac tggttgtgcc gatagtgaca taagccgtat ccggggcgac
    13561 ttcttgctgg gcatagcccg tgacggtaac gacagacggt tccggaccag ctgccagggc
    13621 ctggggcgca gccaggacca gggacgatgc cagcatagcg gcgacgacaa ggcctttcaa
    13681 gggtttatat ttcataagtt aagacctcct ttttaagata ttcctattat accacggcag
    13741 ggggatgtac gtctatgtgg gataaaatgg cgaaattgtc tatgataaat gttacgaaag
    13801 tgttgcattt tttttcgcgg cgtactataa tagccacaga tacatagaaa aagctagggg
    13861 agccttcggg ctgagaaaag tgacggatta cacttgaccc tttacctgat gcgggtaatg
    13921 ccgccgtagg gaagctttac gggattataa agctcacctg cggaggaccg ccaggcgagc
    13981 ttttttattg cattattaag gattattttt atataggaag gatgtttgta atggattaca
    14041 caacacagat ggacgctgcc cgcaaaggca tcgtcacgcc gcagatgaag attgtcgctg
    14101 aaaaggaaca catgcccgtt gaaaaactca tggccctcgt cgccagcggg aaagtcgcca
    14161 ttccggccaa caaagtccat accagcctgt cgccggaagc tgtcggccag gactgccgca
    14221 cgaaaatcaa tgtcaacctc ggcgtttctc cggaacacaa caaccacgaa gaagaactgc
    14281 agaaagtcca gaatgccatc gacatgaaag cagaagctat catggacttg tccaatttcg
    14341 gcaagacccg tgacttccgc cgcaaactgg tcaagatgtc tacggccatg atcggcacgg
    14401 tccccatgta cgatgcggtc ggcatgctgg aaaaagactt gaaagacatt accgtcgatg
    14461 aattcttctc cgtcgtcgaa cagcacgcag aagatggcgt cgacttcatg accatccact
    14521 gcggcatgaa ccgcaagacg gcacagcgca tcaaagacaa cccgcgcctg accaacgtcg
    14581 tttcccgcgg cggttcgctg ctcttcgcct ggatggaaat gaacaaccag gaaaacccgt
    14641 tctacgaata ttatgaccgt ctcctcgaca tctgtgaaaa atacgacgtc accctcagcc
    14701 tcggcgatgc ctgccgtcca ggctgtaccc atgacgctac cgacgccgct cagatcgaag
    14761 aactcatcac cttgggcgaa ttgaccaaac gcgcctgggc ccacaacgtc caggtcatga
    14821 tcgaaggccc gggccacatg gtcctcaacg aaatcgcagc caacatgaag attgaaaaac
    14881 gcctgtgcca cggcgctccg ttctacgtcc tcgggccctt ggtcacggac atcgcaccgg
    14941 gctatgacca catcaccagc gccatcggtg gtgccatcgc cgcttcctgc ggtgccgact
    15001 tcctctgcta tgtcacaccg gctgaacacc tgcgcctgcc gaccgtcgac gacgtcaagg
    15061 aaggcatcat cgctgcccgc atcgcagccc acgctgccga cttggtcaag ggcatcccgg
    15121 gcgctcagga atgggatgac gccatgagca aagcccgcgt cgacgtcgac ttcgacaaga
    15181 tgatttccct gtccatcgac ccggaaaagg cccgcagcta ttacgaatcg agtaaaccgg
    15241 aatgtgaagg cacctgcacc atgtgtggta agatgtgccc ggcccgtacg atgaaacgca
    15301 tcctcgccgg cgaagacgtc tccatccgct aggctccaac ctaaatttac ctcatagata
    15361 ctgaaaaagg cccgtcgcca gatgacgggc ctttttcagg tacagaccgc cgacctgtag
    15421 taggggcttc cacgtgggaa
//
LOCUS       DAWABZ010000036        14780 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_1113_length_14780_cov_3.208081, whole genome shotgun sequence.
ACCESSION   DAWABZ010000036 DAWABZ010000000
VERSION     DAWABZ010000036.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 14780)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14780)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14780
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1113_length_14780_cov_3.208081"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3414 a         4177 c         3876 g         3313 t
ORIGIN      
        1 atttagaggc gccgagggct gcggcatgtt cgctcttgta ggagccgaat ccgacggcga
       61 aggagccttt gtcatcgggg tcgaaatcca agggatggag ttatcatcac tatcccatta
      121 ttcaggaaga acagctgaag aaattgctga aggcaataga tgatttacct gataactaca
      181 tgatcatcta gtttctgcaa gtcctagtta tctgaataca tggctgacaa aaatgtgcaa
      241 aaagatagga ataccccata ttggtgttca ctctttgcgt catatggccg ctacgtatgc
      301 tctgaatgaa ggggcatctt taaaataaaa ctgttgtaat aacgaaaaat ctgctacaat
      361 aaagctgtat tagtctgtgt tagcagatgt tcacaaagga ggttcctcat gaaaacatta
      421 gatgaattgt accagcagat gcttaaacgg gctgctgacg gtaaaccggt cggtgtcgat
      481 ggtgacccga aattgatcga ttgtctggct catcccgacg accatcccct gatctggcgg
      541 gtcaagccct gtctctgtcc gcccgatgaa ccacatcact gccaggaagc ctgtgactgg
      601 ggcgctatca cgtccggtcc cgatggcatc tccatcgacg acagccggtg cgtcggctgc
      661 caggcctgcg tcgatgcctg caagctcgat accctgaaga cgcggaaaga ccttatccct
      721 gtcgtccagg aactgcacga ctacgacggc cctatctacg ccttggttgc tcctgccgta
      781 tccggtcagt tcggtcccga tgttaccatg gggaaactgc ggacagcttt caagcgcctg
      841 ggtttcacgg gtatgctgga agtcgccgtc tttgccgata tcctgacatt gaaagaagcc
      901 ctggaattcg acaagaacat caacaatgaa gaccagttcc agctcaccag ctgctgctgc
      961 cccatgtgga ttgccatgat caagaagctc tatcaccagc tcctgcccaa cgtcccgggc
     1021 tctgtctcgc ctatgattgc cggcggccgt acagttaagg ccctgcatcc taatgccaag
     1081 actgtcttca tcgggccctg cctggctaag aaagctgaac ggaaagaacc ggacctggta
     1141 ggcgctattg attacgtcct gacctatcag gaactgcgcg acttcctgac ggttacggac
     1201 atcgacctgg catccctgcc ggaagacaac gtgccccacg cttcggaagc gggcatctgc
     1261 tacgcctatg ccggcggcgt ctctgaagcc gtcacggaaa cggtccatca gctgaacccg
     1321 gaccgcaaag tcgacatcaa gacgcgcaaa gccgatggcg tcccggcctg caaggccatg
     1381 atcaacgaca tcctggccgg caaccgcgat ggcaacttct ttgaaggcat gggctgtctg
     1441 ggcggatgcg tcggcggtcc ccgggccatc atcaagaaag aagaaggcaa ggtcaacgtc
     1501 caggcctacg gcaaacaggc ggcctacaag acacccatgg aaaatcccta cgtcatcgaa
     1561 ttattgaaga aactcggctt cccgacagtc gaagaattcc tcgaacggag ccacctgtac
     1621 gaccgtattt ttgactgatg atgggtatgg tcatcatgta gtgacctttt tcgtcagagt
     1681 ttctaggggg cgccccacgt ggcgccccta cagaaaaaag ggctgtcgca tctgcgacag
     1741 ccttttttta cacggcctgc gaactgcagc cttcaagggc catttaccaa tgccggctct
     1801 cttttaatgc tgcatattgg gccaggaggt ccggcttttc cgggctctga taggtatagt
     1861 cgggcatggc ggctttggct ttttcgacag ccgctttgaa gtcgtagtag acctgccatt
     1921 tcgattcgac cagttcgtgg ccggtctggc gcaggagaaa ggccgggtga tacgtcggca
     1981 tgatgtcata gccgcgccac gtgaaccact tgcctcggtt gcgcatgatg ctggcggcgc
     2041 ggccatagaa atactggaag gcgactttgc ctaggcagac gatgactttc ggctggacca
     2101 gttcgatttc tttgaccaaa tggatgtccg cgcagtggcg cccttcttcc agggtcggcg
     2161 tgcggttgtt cttgggccgg cacttgacga tgttggtgac atagacgtgg tcccgcgtga
     2221 gtccgacgct ccacaaggcc ttgtcgagga gctgccccga cgggccgacg aggggaacgc
     2281 cgtattcatc ttccacaccg ccaggccctt cgccgacgaa catcagcggc gacgcacaat
     2341 cgccggaata gcgcgtcggt ccgcgattgc cttcgtcccg taagtgacag gctgtacagc
     2401 tcatgagttc tgtcagccaa tctttcatct tttcattttc ttccatgata ccccttcttt
     2461 atgcgataat taattttact ataacaaggc ggccgcctct tttcaagtcg tccagcggta
     2521 ttctaaaaaa atacttttta catactatag tatggtatat aataaagcta aattgacatg
     2581 aaaattaaag aaagggggaa gtgcagtgaa aatctcactc attcaatttc ctatcgtccc
     2641 cggcgacagg ctggccaatt tcgaccaggt ccggcgccgc gtccaacagg cggccagggc
     2701 tggttcggat atggccgtgc tgcctgaatt gtgggacctg tcgttctacc cgcctgatgt
     2761 ttatgacctg gccgatgaag agggccgcca ggcccaggcg tttttgcagg acctggctgc
     2821 gtcctgtcat atccagctca tcggcggctc cattgtccgc cgtcacgacg ggtgcctgta
     2881 caatacgacg tatatcgtcg atggagaggg gaatcgcgtc tcatcgtatg acaaatgcca
     2941 tttattcacg cctggtgggg aagagaaggt cttcgcgccg ggagatcact tgaatacttt
     3001 cttcctgggc gacatcccta tggcgtcgat tacctgttat gacctgcgct ttggcgaatg
     3061 ggtccgcatg gcggccctgg ccggtgccca gatcctcttt gtcccggccg cctggcctca
     3121 tcccagactg gctcattggc agatactcaa tcgggcccgg gctattgaga accagttctt
     3181 cgtcgtcgcc gtcaacagct gcggtacctg cggtgactac cgcttctgtg gccattctat
     3241 gattatcgac ccgtggggcg aagtgctggc ccaaggagga gacggtgagg aaatcgttac
     3301 cggcgaaatc gacttgtccg tcatcaaaga catccgcagc cgcatcaatg tctttcgcga
     3361 ccggcggcct gagttatatc atttagaagg gaaacggtaa cgtggaaggc gtattgcacg
     3421 gtttacaagg tatttttgaa gtcctcttca tcatttccat cggcttcatc ttggccaaga
     3481 aaggctggtt cgggccggac atgagcgctg tctttaccaa actggtcatg aagataagcc
     3541 tgcccttata catgctctgc cagctggaaa aggactttac ccatgattcg ctgctccgca
     3601 tcgcgccgga cctgctcctg ccctttgcgt caatcctttt ggcctatgcc gtcggccgca
     3661 tagcggccaa agtcctgcac atccgccagg accggcaggg cgtcttcatc acttgttttt
     3721 tcattgccaa taccatcttc atcggcctgc ccgtcaacct ggccctgttc gggacgcaga
     3781 gcgtgccgtc ggtcatgctc tattacatgg ccaacacgac catgttctgg accctcggcg
     3841 tctaccatat cgtcaacgac agtctgggcg gcaaggggaa tatgcccttg ttttccctgc
     3901 agaccctgaa gaaagtcttt tcgccgccgc tcctgggctt tttggctggc ctggtcctga
     3961 ttatggccaa tatcaagctg cctgatttcc tgctgacgtc gttccaatac gttggcaata
     4021 tggcgacgcc gctgtcgctc atggtcatcg gcattgaaat gacgggcatt tctctggcgt
     4081 cggtccgctg ggaccgggac atcatcggcg ccctcgtcgg ccgcttcctg gtctgtccgg
     4141 cctgcgtcct ggtgctcctg cccttcatcc cggtcacgcc catgtcggcc caggtcttta
     4201 ctatgcaggc cagtatgccg gccatgaccc agatgacagt cgtcgccaag tccgtcggcg
     4261 ccgatgtccg ttattcgacg gaagtcagct ttatcaccgt cgtcatgggc atcgtcatca
     4321 ttcccttata tatgtttatc attggataga aggagttttt ttagaaaatg aataccctcg
     4381 aagatttgaa gaaagctttg aaagcccatg aagcccaggc ttttcagctc aactatacca
     4441 ttgctgacga cccggagctg tccggtgaag aatatcatgc ctgccggcac tacgtcgatt
     4501 tgtgccgttc tttgggcatg gaagtggaag aacagttcac cggtcagccg acggctttcc
     4561 gagccatcgc tcatcgggcc gataaacccg tcttcaagat ggcccttttg gcggaatatg
     4621 atgccctgcc cggcgtcggc cacggctgcg gccattcggc caatggtgcc atgagcttcc
     4681 tggccgctgc ggccttaaag gatttgaagg atctgcctgt cgacgtcgac ctcatcggca
     4741 caccggacga agaactgcgc ggcggcaagg tcatcatgtg cgaccagggc gtcttcaagg
     4801 actatgattt cgccgtcatg gtccacgtgt cgccgacccg gaccaatgcc aattcccatt
     4861 tcctggccct cgacgactac cgcattgcct tccacggcca gacggctcac gccgccggtg
     4921 aaccctggaa cggccgcaat gccctgaatg gggccatgct ggccctgcac gccgtcgata
     4981 tgctgcgcca gcacgtccgg ccggaaacgc gaatcggctc gtatatcgta aacggtgggg
     5041 ccgcctccaa cgtcgtcccc gattacgccg aagtcgaatg ttgtatccgc cacagcgaac
     5101 gggaatatct cgataccgtc gtggccaagg tcatgaactg cttcaaaggg gcggccattg
     5161 ctacggaaac gacatatgac gtcacccaaa tgggctgcaa attcgataac atggtctgga
     5221 atgaagcggc gacgaaagcg tcggaacagg tcctccaggc catggggatc ccttacgaag
     5281 aaccaaagga ttgcggcagc tccgacgtcg gcaacgtcag ccatcagtgc ccggccgtcc
     5341 acgtccacct ggccttgggc gacgtcccca tgccggaaca ctcgacggac atcgccaatg
     5401 ccgtcaaggc caggaccatc gaaccgacca tcgtcaaagg cgccgaaatc atggcacgcc
     5461 tggccctgct cttcgcccag gacacggccc tgcgccagca gatgaaagat gagttcacgg
     5521 cgaagagcct ctcctgatag aggtagtttg taggggctcg cacatgcctt ttcagcctgt
     5581 caggtcatga ctgcaaggaa tggcaagacg gctgctgaaa cggaccgctg tagagggcga
     5641 gcccgttagg tatatgtgaa atacttggaa aacggatttg cgtgaacggg tcgcccacgt
     5701 ggcgacccct acagaaaaaa ggagctgtcg cgatgcgaca gctccttttt tcacgctcta
     5761 tatattcagt ttcaggtata ccttagaaga caccttgtgc cagcatagcg tcggcgacgc
     5821 gttcgaaccc ggcgatattg gcaccggcta cgaggttgtg gcccaggccg ttgcgggcag
     5881 ctgcgtcttt gcagttctgg taaatggtgt tcatgatttc gtggagacgg tcatcgactt
     5941 ccttggcagt ccataccagg cgttcgctgt tctggctcat ttccagggcc gatgtagcga
     6001 caccgccggc gttgacggct ttcgacggag cgacgatgat gtctttctga tcgagtaaga
     6061 atttcagggc atcgttggtc gtcggcatat tggcgacttc gatgtagtag cggatcttgt
     6121 tggcagcaat ctgtttagcc tgttccatat ggacgtcgtt ctgcatagcc gacggcatga
     6181 cgatatcgac tttgcggccc cacggttttt cgcccgggaa gaattcgacg ccgaatttat
     6241 cggcatagtc ctggacgcgg ttgcggccgc tgttgcgcat ttcgacgatg tagtcgatct
     6301 tttcctgcgt agcgacgccg tccgggtcat agatgtagcc gtccggaccg gagagggtaa
     6361 cgactttggc acccagttcc gtagctttct tgcagatgcc ccaggttacg ttgccgaagc
     6421 cggcagcggc gatagtcttg cctttgatgt cctggccgtc gtctttgaga acgttttcca
     6481 ggaagtatac ggcgccgtag cccgtagctt ccggacggat gcgggaaccg ccgaagctga
     6541 agcctttgcc cgtgaggaca ccgttttcga agcagccttt caggcggcgg tattcgccga
     6601 agaggtagcc gatttcacgg ccgccgacac ccatgtcccc agccggtacg tcgacatcgg
     6661 gaccgatgta gcgatagaga gcctgcatgt agctctggca gaaacgcatg atttcagcgt
     6721 cggacttgct cgtcgggtcg aagtcagccc cgcctttggc gccgccgata ggcaggccgg
     6781 tcagtgcgtt cttgaaaatc tgttcaaagc ccaggaattt gatgatgcct gagtatacgt
     6841 tcttctggaa acgaaggccg cctttgtagg gcccgatagc gccgttgaac tggaagcggt
     6901 agcccgtgtt ggtgtggtat ttgcctgcat catcctgcca gacgacgcgg aatgtgaatt
     6961 gccgttccgg ttcgacgaga cggttgagga ggtcgaactt ttcatattcc gggtggcgtt
     7021 cgacgacagg gtccagggag ctgaagactt cttcgacggt ctggacgaat tccggttcgt
     7081 ttgcgtgttt cgtgcgtaca ttttcaatga cagattccaa atacttattc atatgtaatc
     7141 acctcattta aaattaatta tacctaaaac tgatttctac aattacataa tagcatgttt
     7201 ttccgtcctt gcaaatacca caatgaggaa aagatttttc catgatggca ctgcattgcc
     7261 actatgtgaa agaggacgtc aaaattcaag gatttttaag gtataagata aacgttatag
     7321 taagttaaag ggaggtattc gttaaaaata attgcaatat aaatcaaaat catggtataa
     7381 atagaactat acttattgag gaagaagggg gaagacggcc ctttgtcttt tgctggagga
     7441 ataattgtat acaccggtta aacaaaatgg agaggaaggt gacgaaaatc agaaaaagaa
     7501 cctagtcacc ctgacaatga tgatatacaa tagaaagtaa ataagccata caacatatac
     7561 tatataagaa agagaggtac gcaacatgga aacgtacgat acctattccc cggaatattt
     7621 gcgctatttg gagctcctgt ccaaggagta tcccacgcag gcggcgacgt tcacggaaat
     7681 catcaatctg caggccatcg tcaacctgcc gaaagggaca gaacacttca tgagtgacct
     7741 gcacggtgaa tacgaagcct tctatcacat cctcaacaac tgctccggcg tcatccgcga
     7801 aaaagtggaa atgatttttt ctgacaagat gagcaaggcc gaacaggacg acttgctgac
     7861 tcttatttat tatcctaagg aaaagctgaa catgctctac cgcgaaggca aggtcaacga
     7921 tgactgggcc cggacgacgc tggaacggat gatcgacctg gccaagctct tgtcgtcgaa
     7981 atatacgcgg tcgaaagtcc gtaaagccat gccggaagtc ttccgcttcg ttatcgatga
     8041 actcttgcac gcccagcccg atgaagacca gaaccagctg gtctatcaca tgaagattct
     8101 cgacaccatc ttggaaacgg gaagtacgcg ccaattcatt tacgctttgg ccaatctcat
     8161 caaacggctg gccgtcgacc acctgcacat catcggcgat atctacgacc gcggcgctca
     8221 tgccgacaag atcatggaca cgctcatgca tcatcattct ctggacatcc agtggggcaa
     8281 ccacgacgtc ctctggatgg gtgctgctgc cggcaacgcg gcctgcatcg ccaatgtcct
     8341 gcgcaataac atccgttacc ataatatgga aatattagaa agcggctatg gcatcagcct
     8401 tcggcccttt gccctctttg ccttgcagac gtataaagag gacgacggca ttgagcccat
     8461 ggtcaaggcc atcaacgtca tcgtggccaa gctggaaggt cagaccattt accgccatcc
     8521 cgactacaat atggacgacc gcctgctgtt ccacaagatt gattgggaaa aaggcgtcat
     8581 cactctcgat ggcaagacgt atgaattgaa gaccaaggac ttcccgacgg tcaatcccca
     8641 ggacccgtac acgctgtcgc cggaagaaca ccagctcatg aacgacatcc aggccgaatt
     8701 taccgaaagc gaacgcctgc agcgccacat ccgcttcctc tacagccacg gctcgttata
     8761 ccgctgcatg aataacaacc tcctgttcca cggcggcctg cccctcaacc aagacggcag
     8821 ctttaaggaa atctacctcg acggtaagcc gtataagggc aaagccttca tggataaggc
     8881 ggaacacctc atccgccggg cttatgacca gcgcgatact gacagcctgg actatatgtg
     8941 gtacctctgg tgcggtgccg attcccctgt atccggccgc atcgtcaaga cttttgaacg
     9001 gagttacatc atagacgaat cgacgtggaa agaaccgcag aatgcctact accgcctgaa
     9061 tcgcgataaa gacgaatgca tcaagatcct ccacgaattc ggcatcgact cgcctcaggg
     9121 acacatcatc aacggccata cgccggtcaa ggtcaagaaa ggcgaaagcc ctatcagggc
     9181 ggaaggcaag gaaatctgca tcgacggcgg tttctgcaag gcctaccagg ggtcgacggg
     9241 cattgccggc tacacgctca tcttcaactc ccacggcata cgcatcaaat ctcactaccc
     9301 ctttgaagat gtctaccagg cgttgatgaa caacgtcgac atcgattccg aatcgacaca
     9361 ggtcgaactg gaaccgaagc gcgtcatgat cggcgacacg gataacggca agaaactcct
     9421 ccagatgatc cacgacctga aggccctgct ggaagcctac cgccagggcg tcatcctcga
     9481 acgccgtccg aacccgtagg ggccgtccca aaggggggcc cgcccggttt taagtttgca
     9541 gtttttagtt tgcagcaaag cgtaaataat ttaaagccat cttctacaaa ctacaaactc
     9601 tatactacaa gctataaaga aaaaatccgt gcatgatatt agaaatattc gtgtgacatc
     9661 acccaatcaa ggagaaaata tgaaacgaga atcgttaagc ggactgttac tcattgcctg
     9721 tgcgatactt gccatggcca tctctaattc tcccttcggt cccgcttatg aacgctggct
     9781 ccaggccccg ctgccggcag ggacgcacat gtccctgctg cactgggtca atgacgccgt
     9841 catggcgctg ttcttctttg tcgtcggcgc cgaagtgaag cgggaattcc tctatggaga
     9901 actgaagtcc cggtcggcag cgatgctgcc catggctgcg gccttaggcg gcatgctcgt
     9961 gccggctgcg atttatggcc tgtgcaatgc cggcctgccg acggctggtg gctggggcat
    10021 ccccatggcg acggacatcg ccttttccct gggcgtcctg tccctggcgg ctccgaaagc
    10081 gccgcgcagc ctcgtcgtct ttttgacggc cctggccatc gtcgatgatc tcggcggtat
    10141 cgtcgtcatc gctgtctttt atgccggttc cctcaacctt ccggtcctgg ccggcggcct
    10201 catcgtctgg ctgctgatgc tggctgggtg ccgcaggaat tggcaatcag cctggtttta
    10261 cgctgtggga ggtatcctca tctggctggc cttcttctac ggcggtatcc atccgaccat
    10321 ggccggcgtc ctcacgggct tcgccatgcc ggccgtgccc ggcaaggatg gaagggaagg
    10381 cttgctgctg cgggcagaac acgccctgac gcctgtctcg gctttcgtca tcatgcccgt
    10441 cttcgccctg gccaacgccg gcatccgcct cgacctgagc ggcctgaccg gcctggcgtc
    10501 ggcaccggga ctgggcgtcc tggctggcct ggtcctgggc aagcccatcg gcatcggcct
    10561 ggccgtctgg ctgttgacgg ccttgaaaaa agcgtccctg ccagaagggc tgacgatgaa
    10621 acacttctgg gccgtcggcc tcctggccgg tatcggcttc accatgtccc tgttcatcgc
    10681 cggcctggcc tttcccggca gtccggtcct gatgacggcc aagaccgcca tcaccgccgc
    10741 gtccctcacg gcggccatcc tcggcggctt cctggtcaac cgcgtaagcc gatagccttc
    10801 ctgtaattat cggtaggggg ctcacacgtg gcgagcccgc cgcgaatagt cagccatata
    10861 ccaagcgggc caatgtggcg tggttttgcg gcctgcggcc tgcggtctgc gaacagcggg
    10921 cgcctttggc gcccatcatt gacgtaatac gttcatgtcg ttataatgaa gagtaactta
    10981 tcatttttta tacaaaagga gcgaattcga tgaatattat tgatgaattg tcttggcgcg
    11041 gtgccgtcaa tcagatgaca gatgaagaag ggttgcgtaa gctcacggaa gaaaagagca
    11101 tttccctcta ttgcggtgtc gacccgacgg gtgacagcat gcatatcggc cacttgattc
    11161 cgttcatgat gcttaaacgc tttgccaatg ccggccatca tccgtatgtc gtcattggcg
    11221 gcggtaccgg tgcaatcggt gacccgagcg gccgcaagac ggaacgccag ctccagactc
    11281 tcgataaaat caaggccaat gccgaaaaat tgaaggctca gttcatgcat cttttcggcg
    11341 attcggaaaa catcaccttc gtcaataact atgactggct cagccagatc agcctcctcg
    11401 atttcctccg cgattatggc aaactgttca gcgtcaacat catgctcagc aaggaagtcg
    11461 ttgccagccg tctggaagca ggcatttcct ttacggaatt ttcctaccag attctccagt
    11521 ccatcgactt tgaacacctc ttttcccata acgacgtcca gctgcagatc ggcggcgccg
    11581 accagtgggg caacatcacg gccggtatcg acacgatccg caagatgcac ggcagcgatg
    11641 ccaaagccta tggcctgacc attcccctca tgctgaaatc tgacggcacc aagttcggca
    11701 agaccgccgg cggcgctgtc tggctcgacc cggaaaagac atcacccttt gaattctatc
    11761 agttctggct caaccaggat gatgcggacg tcgtcaaata cttgaaatac ttcacctttc
    11821 tcagcaagga agaaatcgac gccctggccg aatcggtcga aaaagaaccg gaaaaacgac
    11881 tggcccagcg ccgcctggct gaagaagtca cgactttcgt ccacagcccg gaagccctga
    11941 aggaagccca gaacatcacg gaagccctgt accacggcag catcgccgac ctgtcggaaa
    12001 gcgaattgga acaggccttc ggcaagatgc cgactgtcga cgtttccaac gaagataaag
    12061 acgtcgtcaa ctggctcgtc gattcccaga tttacaaatc gaaacgccag gcccgcgaag
    12121 acatcaccca cggtgcggtc accatcaacg gcgtcaaagt cacgtccctc gacgaaatcg
    12181 tcaaacctca caagaattcc aacggcaagt tcgtcatcgt tcgcaaaggg aagaagaaat
    12241 atactttggc ccgggttcat cagtaagggt agattttaga aaattgttaa aatagaatat
    12301 tgacagaagt gctttgctat gctataataa ctcattgcaa aatgttcaat tgatattatg
    12361 agatacatgg aggaagcgct tccggtgagc aaaaaattaa gcaaggttca aatcgtctga
    12421 gagccttgct tttttgtcgc tttacacggc gctgtacaac gctatcataa tgatttactt
    12481 tttataaggg gtgaagaatc agcatgttca aacctgtaca agtagggaaa aggactcgat
    12541 acacgtatgc taagattaac gaggtcttga aaatgcctca tttgttggat ttacagaata
    12601 aatcctacga atggttcctg gaaaagggac tgaaagatat cttcaaagac atttctccca
    12661 tcgaagatgc atcgggtaac ctgtctttat ccttcgagaa ttttaaatta ggggaaccga
    12721 agtacgacat tcgtgaatgt aaggaacgcg atacgacata cgccgcaccg ctccgcgtcc
    12781 agatccgtct tgtcaaccac gaaacaggtg aaatcaagga ccaggaagta ttcatgggtg
    12841 atttcccact gattaccgat acaggtacct tcattatcaa tggggcagaa cgcgttatcg
    12901 tcagccagct cgtccgttct ccgggcgtct actatggcca cgaaatcgac ccgatgggca
    12961 tccgcctgta caattcgacg gtcattccga accgtggtgc ctggatcgaa ttggaaacag
    13021 acgccaacga catcgtctat gcccgtatcg accgcaaccg caagattcct gtcacggtcc
    13081 tcatccgcgc gctgggctgg tcgtcgaacg ccgacatcct cgaactcttc ttcgacgata
    13141 aacgcctgaa ggctacgttt gaaaaagata cgacggaaaa tcaggacgaa gccctcgttg
    13201 aaatctataa gaaactccgc cccggtgaac cgccgaccgt tgaaagtgcc cgcaatctct
    13261 ttgaaggcct cttcttcgac ccgcgccgtt atgatatggc ccgcgttggc cgttacaaag
    13321 ccggcaagaa actcggctgg gaacgccgcc tctttggcct gaccttgcac gaaccgatcg
    13381 tcaacccgga aacaggggaa gtcatcgtcg atgcccatac ggaaatcggc tcggctgaag
    13441 ttgaaaggat ccgcgaaagc ggcgtcttcc atcgcgaaat cgccgacggt gcgcccgccg
    13501 gtgacggcgg cctcgtcgaa gccttcgtcg aaaaaacgga cggctcggtc tacaagatca
    13561 tctgcaacaa cagcgacctt ccgtatgacc accgcacgat tacccgcgaa gacatcatcg
    13621 cccacatcag ctatcttctc aacctcatgg acggcttcgg aactgtcgat gacatcgacc
    13681 atctgggcaa ccgccgcctg cgctgcgtcg gcgaactcct gcagaaccag ttccgcatcg
    13741 gcctgacccg tatggaacgc gtcgtccgcg aacgcatgac catccaggat gccgaaagca
    13801 ttacgcctca ggtcttgatc aacatccgtc ctgtcgtcgc tgctatcaaa gaattctttg
    13861 gttccagcca gctgtcccag ttcatggacc agacaaaccc gctggcagaa atcacgcaca
    13921 aacgccgtct gagcgccctc ggccctggcg gtctgtcccg tgaacgcgca gggttcgaag
    13981 tccgcgacgt ccatcattcc cactacggcc gcatgtgccc ggtagaaacg cctgaaggcc
    14041 cgaacatcgg cttgatttcg tccctggcct gcttcggtaa agtcaacgaa ttcggcttca
    14101 tcgaagcccc gtaccgccgc gtcgataaag aaacggtcgt cgttacggaa gatgtccact
    14161 acatcacggc tgatgaagaa gaacagtacg tcatcgccca ggccaacgaa ccgttgaccg
    14221 aagatggcca tttcgtccac gaacgcgttg cctgccgtca cggtgaagat acccgtgaat
    14281 tccgggctga ccgcgtcgac ttcatggacg tatcgcctaa ggaagtcgtt tccgtcggta
    14341 catccatgat ccccttcctg gaaaacgacg atgccaaccg tgccctcatg ggtgcgaaca
    14401 tgcagcgcca ggcagtacct ttgctgcgca cacaggctcc gctcgtcggt accggcatgg
    14461 aatacaagac ggcttgtgac tccggcgtct gcgtcctggc aaaacacagc ggcgtcgtat
    14521 cccgcgttac tggcgacacc atccaggtcc gcacggattc tggttctgtc gatacatata
    14581 aactcatcaa attcgtccgc tccaaccaga gtacctgcat caaccagcac ccgttggtct
    14641 ataagaacga aagggtcacg gaaggtcagc ccctggctga cggcatggct acggacggcg
    14701 gcgaattagc tcttggttac aacatcctcg tagcctacat gccttgggaa ggctataact
    14761 acgaagacgc cgttctcctc
//
LOCUS       DAWABZ010000037        14664 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_1124_length_14664_cov_3.574577, whole genome shotgun sequence.
ACCESSION   DAWABZ010000037 DAWABZ010000000
VERSION     DAWABZ010000037.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 14664)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14664)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14664
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1124_length_14664_cov_3.574577"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6738..6747
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14120..14129
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3464 a         4275 c         4061 g         2844 t
ORIGIN      
        1 tatgccggca cgttcttcgt cttcagcgat tacatgaaac ccatgatccg cctggctgcc
       61 ctcatgagcc tgccgacgac gtacgtcctc acgcacgaca gcatcggtgt cggcgaagac
      121 ggcccgactc acgaaccgat tgaacagctg gccatgctcc gctcccttcc taacgtcaac
      181 gtcttccgtc cggctgacgc tacggaaacg gctgccggct ggtacctggc tgcgaccagc
      241 cagaagacgc cgacggccct cgtcctgacc cgccagaacc tgccgcagct cgccggcagc
      301 agcaaagaag ccctgaaagg cgcttacgtc atcgccgatg ccaacaaggc cgttcccgat
      361 gccatcatca tggccagcgg ttcggaagtc gaactggctg tcagcgctaa agacgaactg
      421 gctaaagaag gtgtcgacgt ccgcgtcgtc agcatgcctt gcatggaact ctttgaacag
      481 caggacgaag cctataaaga acaggtactc ccgaaagctg tccgcgcccg cgtcgctgtc
      541 gaagccgctt cggacttcgg ctggggtaaa tacgtcggcc tcgacgggac gacggtttcc
      601 atgaaatcct tcggtgcctc cgctccggcc ggcgtcttgt tcaagaaatt tggcttcacg
      661 acagaaaacg tcgtagccgc cgtaaagaaa gtcgtaaaat aatcgtggtt cgtggctcgt
      721 agggaacgtc caaaggcgtc ccgcagaccg caggtcgcag ttagccgttc gtaggggccg
      781 tcccaaaggg cggcccgccg cgtctttgcg ggattctcac atatacggaa acgggcgccc
      841 cacgtggcgc ccctactagc cactaacccc tagcccctaa ccacttaaaa ggcgctgtct
      901 tcatgcgaca gcgccttttt catttgtcaa ggcttgattt ttcttctata ttttattata
      961 ctagatacaa gaagaaaccc tactgtgtac gtaaatggtc tcgatgtttt gaaccaacac
     1021 tttttacatt gggagttcgg gctctatatc gttaggcaat gccttcactg gactgtcgac
     1081 ccttatagga gaacacccac ctgcttaggc aggctcaaaa ctcggtttat aaacggcatg
     1141 gtggggctct tttcttattc gagaatttag ttatttcgtc cgccgctgag gagacttggc
     1201 ggcgttctat ttagtctgga gagcaaacaa atgagcggag acgatttatt ttctatggct
     1261 gatgaacgga gcggccggga ctatgcgccg ctggccgtcc gcatgcggcc ggaatctttg
     1321 gatgacatct atggacagga cgacctcatc ggcccgggca gttttctgcg gaccctcatc
     1381 gagaaggaca cgatcccgtc ccttttgttt tacggtccct ctggcgtcgg caagacgacg
     1441 ctggcccatg tcattgccaa tgagacggac agccgtttcg taaccctcaa cgccgtcacg
     1501 gccgggacgg cggaactgcg caaagtcatc gcggcggcgc aggatgccat ccatttatat
     1561 caaaagcgga cgatcctctt catcgacgaa atccaccgct tcaataaaag ccagcaagac
     1621 gtcctgttgc cgtatgtcga agacgggacg gtcatcctca tcggggcgac gacggaaaat
     1681 ccttattttg aagtcaaccg ccccttattg tcgcgcctgc gcgtcatcca gctgaagccc
     1741 ctgccggaac aggccgtcat ggccgtcctg cggcgggctc tgacggataa agaaaaaggc
     1801 ctgggctcgc tgggcatcac ggccagcgat gagatgctgg gaaccctggc gcggctggcc
     1861 gaccgcgatg cccgcgtcgg cctcaacctg ctggaacagg tctgcatggt cgtcccggct
     1921 ggcggccata tcacacacga cgctatcgaa aaagtagccg gtcataaggt ctatacgtat
     1981 gacaagaaag gcgatgccca ttacgatacg gtatcggctt tcatcaagag tatgcgtggg
     2041 tccgaccccg atgcggccct gcactatatg gcccgtatga tcgacgccgg agaacagccg
     2101 tccttcatcg cccgccgcct ggtcatctgt gcggctgaag acgtaggact ggccgatccc
     2161 caggctctgg tcatcgccaa tgccgctgcc caggccgtcc agttcgtcgg ctggccggaa
     2221 gggcgcatca tcctgtccga agccgtcatc tacgtggcct gtgcgccgaa gagcaacagc
     2281 gcctacatgg ctatcgatgc ggctctggcc gacgtccgcc ataaggactg tggcgatgtg
     2341 cccgattatc tacgcgacag ccattacagc ggcgccgccg ctctgggcca tggcctgacc
     2401 taccagtacc ctcacgattt cgatgggggc tgggtggccc agcagtatct gcccgatgct
     2461 ttgaaaggag cgtcctatta ccgggaacgg ccctatggac aggaagggag catggcagct
     2521 gcctggcata agcgccgtgg cggcaagtaa tctattagta gaaggtgaac tgttttgaca
     2581 aaaggaaaga cccgtaaaac cagccgcacc aagcggacga ctggcacgcg tcagaaggcg
     2641 aagactgcca ggaaggcagg gacgtcgcca cttttgaaat atgaaatcat aggtatttgt
     2701 tgtatcctcg caggcgtctt tgcgacggcc ggcttcatcg gcgtcgatac aggccgcatc
     2761 ggccacagcg tcgacgatat cttatcgtat atcttcggcc tgggccggat cgtcgtcagc
     2821 ctgtccctca tcgtcctggg cctgaaatac atcgtcatcc gcaaggcctg cccagtgtcg
     2881 aaatcgtggg ccgtcgggac gtggatctat atcctcctcc tggggctggt ccatttgctg
     2941 gtcatcccgg aagggacgga attcctgccg cacagcttat ctgtcggcgg gggaatcgtc
     3001 ggggccgtca tggccttggt cctgcggcgg tttcttggat tttttggagc tgtcatggct
     3061 attaccggtg cctgcatcgt caccttcctg gtctggaaga attggtctat ttctaagcct
     3121 gtggctgtcg tagccgacaa ggccggccag gaagcggcca aagtgtcggg taaggccgtc
     3181 gaaggccttc aggacgcatc ccagtccctg aaacagtggc atgaacgccg ccgcatcttc
     3241 gatttgcagg cggttgtagg ggacgacccg ttagggcgtc ccgcttgtgg gaatcccgat
     3301 gattatcccg ctgatatgga acgcccggcc tcaaccattg ccgatgcctg cgacttgggc
     3361 aacggcgtcg aagaagtccg cttcgatgcc agccctatcg aagaagacgt gccttacgat
     3421 tgggccagcg acgattcgta tgaagaagtt ccggaaacgc cggaggaaat gccggaagac
     3481 gcccggcctt acagtcagat acatgaaacc gtcgtcggcg atacagtgcc gacggcccat
     3541 gacgtacagc ccggcccggc agcgccggcg gcttcactgg ccggagaaat ggatgtcctt
     3601 ggccatgata tgggcgacgt caagcccatt gaagtcgaaa agagcagcgt cgctgtcgaa
     3661 accagcgaag aagggagcag tgccgttccc aaagcggctg gcgaaaagac gtaccgcctg
     3721 ccgccggtga gcatgctcaa accggggccg cagcatatca tcggcctgag cgatgaagtc
     3781 cgggaaaatg cccgcatcct ccaggaaacg ctgcagagct tcaacatcga tgccaagata
     3841 ctcaatgcca gccaggggcc gtctatcacc cgttacgagc tggaaccggc agcgggcgtc
     3901 aaagtcagca aaatcgtcca cttagccgat gatatcgcct tgaaactggc cgccacggat
     3961 atccgcatcg aagcgccgat tcccggcaaa gcggccgtcg gcatcgaagt gccgaataaa
     4021 aagctgaccg gtgtcaatct gcgcgatgtc atcgacacgg atacgttccg caaggccgcc
     4081 ggcggcgtcc cggtctgcct gggcaaagac attgccggca accccatcgt cgccgacctg
     4141 accaagatgc ctcatctcct ggtagccggc tcgaccgggt ccggcaagag tgtctgcatc
     4201 aacaccttca ttgccagcat cctcttcaag cagcggccgg aagacgtcaa gctcatcctc
     4261 atcgacccga aggtcgtcga attatccaat tacaacggca tcccgcactt gctgacgcca
     4321 gtcgtcacgg acccgaagaa ggccgccagc gtcctgcgct gggctgtccg ggaaatggac
     4381 gaccgctata agcgcttcgc catcacccat acgcgggata tttcccgcta taatgaactc
     4441 catccggaag acacgatgcc tttcatcgtc atcatcatcg acgaattggc cgacctcatg
     4501 atgatggcct cagacgacgt ggaaaagtcc atcatccgcc tgggccagaa agcccgtgcc
     4561 tgcggcatgc acctcgtcct ggcgacacag cggccgtctg tcgatgtcct gacgggtctc
     4621 atcaaggcca atgtcccgag ccgcattgcc ttcgccgttt ccagccaggt cgattcacgg
     4681 actatcctcg acatggccgg tgcagaaaaa ctgattggca agggcgacat gctcttctat
     4741 cctctcggtg cttccaagcc tctccgcgtc cagggcgctt tcatttccga ttcggaaatc
     4801 gacgaaatgg tcgaattcat caaggcccag ggcggtccca attacgatga agccgtccag
     4861 aaggcccagt cggaaaatcc cgaagacagc gtcgatttct ttgaagacga cctcatgcgc
     4921 caggccatcg acatggtcct ggaaacgggc caggcttcga cgtccatgct ccagcgccgc
     4981 ttccgcgtcg gctatacgcg ggcagcccgc atgatcgaca tgatggaagc catgcacatc
     5041 gtcggcccca ataacggcag caagcccagg gaaatcctca tgacggccga cgaagtccag
     5101 cagaagtatt tgagctgaac ccctgtaccc ctggccgtat ctgttatata ataaaaggta
     5161 cgattataga gaatggggag aggaacaaca gtgaacgaat cgtcgatcgg agaagtgctg
     5221 cgactggcaa gagaacaaca agggctgaca ctacaagacg tgtccgatgc cctcaatata
     5281 aagcgggaat acctggaagc cctggaaaag gacgaatacg atgccattcc cggcgccgtc
     5341 ttcgtcaagg gctttcttcg caattacggc attttcctgg aactggacgg catcgccctg
     5401 gcccgggaat acaaggaaaa cgtcgaagaa cggacgccgc agccggaagt gcggaccatc
     5461 atgcccgtca aaaagaagaa agtccgcaag gacagcaata aaaagaagaa gcagcgtcag
     5521 ggaaaatggc cggaaatcac tatcattgcc ggcatcatca ttttcctgct gctcatcatt
     5581 tggattttaa tttaaataag gagccatatg cgcgagaaat tcttagtaac cagccgggag
     5641 gacatggaac gccgcggctg ggatcagctc gattttgtct acatcaatgg cgacgcctat
     5701 gtcgatcacc ccggttttgc cgcagccctc atcggccgcg tattggaaag ccggggctat
     5761 cgtgtcggta tcatttccca gcccgactgg catagtgccg aaccgttcaa gaaattggga
     5821 cggccgcggc tggccgcttt gattacagcc ggcaatctcg attccatgct caatgaaaag
     5881 acggcagcca agaaattccg cagccgcgac agctattcgc caggcggcga agccggccac
     5941 cggccggaac gggcgaccat cgtctatgcc aaccgcatgc gggaagccta taaggatgtg
     6001 cccatcatca tcggcggcat cgaagccagc ctgcgccgct ttgcccacta tgattactgg
     6061 tccaataaag tgcgccattc catcctcctg gacagcaagg ccgacatcct cagctacggc
     6121 atgggggaac attccgtcgt cgaaatcgcc gacgctctgg ccgaaggcaa gaccgtcgcc
     6181 gaaatgtacg acatccgcgg catctgctac gtcaccagcc ggccgcccat ttcagataag
     6241 accgtcgtct gtccgtctta tgaagacgtc aagaaggata aattgacctt tgccaaggct
     6301 ttcaagatgc agtacgaaga acaggatccc ttttacggca agactatcat ccagccgtca
     6361 gagaaccgct tcgtcgtcca gacgccgccg gccctgccgc tgacgacagc cgagatggat
     6421 gctatctacg aactgccctt ccagcggcgc tggcatcctg attacgacgc agccggcggc
     6481 gttccggccc tgcatgaggt ccagttcagc ctgaccagcc agcgcggctg tttcggccac
     6541 tgccatttct gcgccattgc cagccatcag ggccgcatca tccagcatcg cagccatgaa
     6601 tcactggtca gggaaacgga gcgcatgacc catctgcccg gtttcaaagg ctacatccac
     6661 gatgtcggcg gcccgacggc gaacttccgc cacgtcgcct gtgccaagca gctcaccgac
     6721 ggggcctgcc gcaaccgnnn nnnnnnncca ctgcatcggc agcgaaacgt gtccgaacct
     6781 caatacgtcc cacgacgatt atctgaagct gctgcgggaa atacgcagcg tcaaaggcgt
     6841 caagaaggtc ttcgtccgct caggcctgcg ctatgactac gtcctggccg accacaacaa
     6901 ggctttcgtc aaggaactct gtcagtacca cgtcagcggc cagctgaaag tggcgccgga
     6961 acacgtcgtc aagcacgtga cggacctcat gggcaaggcc gacgtctatg ccttcctgaa
     7021 gttcaaggac tggttcgatg aagccaaccg ggaactgggg aagaaacagt acctcgtccc
     7081 gtatttcatg tccagccatc ccggctgtac cctgaaagat gccgtcgccc tggccgaatt
     7141 ccttcgggat atgcatatgc agccggaaca ggtacaggac ttcatcccga cgcctggaag
     7201 cctgtcgacg gccatgtact atacaggcct caatcccttg acgggtgaaa aagtctacgt
     7261 cgcccgccgg ccggaagaga aacagatgca gcgggccctg atgcagtata agaacccggc
     7321 caattatgat attgtctata aggccctgtg cctggccggc cgacgggacc tcatcggata
     7381 cggtcccaaa tgcctcatcg cgccgcggcg ccatcaggca gggagacggc ccgataaggc
     7441 cggccggcat gccgctccgc cccgcaggca ggggagggga ccggctaaga ctagaaaatt
     7501 caggtgaagc taatgaagaa attcgtgccc tttatcgtgc tcagcctctt cgtcatcctg
     7561 ctcctgggcc tgtgcgggcc catcctggta cagcgccagc aggaagaagc acagcgggcg
     7621 tatgaagccg aacgccatct cgtcgtcttt tccgacttgc ctcaggatgt caacgacggc
     7681 ctggcccagc gcttttacga acagaaacat ctgcgcgtcc agatctattc caagagcgac
     7741 agcgagatcc gccagtccct gaaggacgac ggcggcgtca agcccgatat cctcatcgcc
     7801 tcggaagacg acttgcaggc tcagaagaaa aacggcatct tgcagcccta cgcctcaccg
     7861 gtcacggata aagtgccgcc gtccatgaag gacccggaac agctctggaa tggcctctgg
     7921 tataacccta tggtcttcgt cctcagccag gcctattacg aacggcgggg catgcagatc
     7981 cagtcctggg acgacctgtt gaccgacccg ggtatggtcc tggctttccc ggacctggca
     8041 tccatggata tggccggcga gttcctctgt tcttttgtgg aaatgagagg catggaagac
     8101 agtgaacgct atctgagctc gctccagagc catgtagcgg cctattctaa gtccatgtcg
     8161 gtcaatgtcc gccgcgtagc cagcggcgaa gccgatatgg gcgtcgccga cgcgtccgtc
     8221 gcccggcagt attacaaaga cggggcgccg atcttcatcc tctatcctca agacggcacg
     8281 tcgtactggc tgaccggtgc ggctgtcacc aactggtgtg aagatggaga attgaccaac
     8341 gccttcatcg actggctcta ttcgcccgat gctattgacg tcttgcggca gagacacatt
     8401 tttttgagcg aagccctgcc gccggccaac ccggaagtcg atgccaaagg ccgggagctc
     8461 gtcttattcc ccgtaaaaaa acagtattct gatcagggcc gccgcgattt gcaggcctgg
     8521 tggatcaaat caattcgctt cggaaaggaa aagtaaagat acatggaaaa aatcggattt
     8581 gtcagcttag gctgctccaa aaaccttatt gatacggaag tcatgctggg catcctccgc
     8641 gaccgccata tggagatcac agaggacctg ccccaggcgg atatcattat cgtcaatacg
     8701 tgtacgttca ttgaaaaagc caagcaggaa tccattgata cgatattgca ggctgcccgc
     8761 tacaagacgg aggggaaatg caagatactc atcgtcacgg gctgcctgag ccagcagtat
     8821 aaagaagatt tgatgaagga aatgccggaa atcgacgccc tcttgggaac cggttcgtgg
     8881 gaccgcatct gggaagccat cgatacggtc aagaaagggc gcaaagcctg cttcatggac
     8941 gatgtcagcc atctctacaa ccagaatacg agccgcctgc ggacgacgcc gaagtacagt
     9001 gcctatgtga aaatcggcga aggctgcaac aacggctgct cgttctgcat cattccccat
     9061 gtacggggca agctgtacag ccggcccgtc gattccgtcg tcgccgaagt acagcagctg
     9121 gcagccgacg gcgtcaagga agtcaacctc atcgcccagg acacgacgag ctatggcgtc
     9181 gacctggccg gtcattccct gttgccggaa ctgctgcgcc agctggtgaa gatcgacggc
     9241 atccgctgga tccgcctgtt ctatctctat ccccactact ttacggatga attgatggac
     9301 ctcatcgtca aggaagaaaa aatctgtccc tacgtcgact tgccgctcca gcacatttcc
     9361 cagtccgtct tgcagcgcat gaaccgccgc gattccgaag ccgatgtgcg cagcctcatg
     9421 aagaagctct gcagtcacgg ccgcaagctg accctgcgct cgacgttcat cgtcggcttc
     9481 cctggcgaaa cggaagaaga attccaggaa ctctgtgatt tcgtcaagga aacggaattc
     9541 gacgatgtcg gcgtcttcac ctattcccgc gaagacggca cgccggcagc cctcatggac
     9601 ggccagattc cggaagaagt caaggaagaa cggtatcatc agctcatggc catccaggcc
     9661 aaggtatcgg aaatccgcaa tcaggaactg gaaggctctg tccatacgat gctcgtcgaa
     9721 agagtagaag aagaaaaggg tatccgccag gctgtcgggc gcattgaaat ccaggctccc
     9781 gacgtcgatg gcctgaccta tctggaagac gcccaggatg tacagcctgg cgatatgatt
     9841 cccgtccgca tcgcccaggg ctttgcctat gacctcgtag cggaacggat ggattaaccg
     9901 aggtgatcgc ttatgttcgt ggaactggtt acgacaggtt ccgaattact gttaggtgaa
     9961 attaccaatt ataactcagc ttacttgtcg aggaaactca atgaaatcgg ctattctgtc
    10021 atctatcata cgacggtcgg cgacaacccg cggcgcatgg aagaagccct gaagacggcc
    10081 ctgtcccgcg tcgacatggt catcaccaca ggcggcctcg ggccgaccca gggcgatatg
    10141 accaagatca tcggcgcccg cctcatggga atgcctctcg tttataatga aaccatcgcc
    10201 gaatcggtcc gccaatgggt cctcgtccat cacggcagca cggccatgac ggaaaaccag
    10261 aagcgccagg ccatggtccc acagggcacc gtcctcttcg ataacggcgc cggtacggca
    10321 ccgggtgtgg ccatgcagca aggcggcaag accctggtcc acctgccggg accgccaacg
    10381 gaaatgcgct ggatgtatga aaaccgcctg aaaccgtggc tgctccagac ttttggcagc
    10441 cagggctgca tcgcttcgcg ctatatcaaa atctatgaca tgggcgaagc cttcatcgaa
    10501 gagaaactca tggacctggt caaggagcaa tcgaatccga ccctggccat gtatgcccgg
    10561 ccaggcttcg tcgaagtccg cctgacggcc cgggctgacc gggaagatca ggcgcggtcc
    10621 ctgctggctc ccctggaagg ggaagtccgc cgccggctgg ctggccagat tgtcagcttc
    10681 gacgacgata ccatggccca tgtcctgggt cagctcgttc gcgacagcgg ctattccgtc
    10741 agcgctgccg aatcgtgtac cggcggcctg gtgggctcac tcatcaccga tgtgccgggc
    10801 gcctccgatt acttccgcgg ctcggccggg acgtactgca atgaaatgaa agaaaagata
    10861 ctcggcgttt ctcacgatac gctggaacgc tatacggccg tcagcgtaca gacggctgct
    10921 gaaatggccg aaggcagccg ccgcctgtac gggacggacc tggccgtcag tacgacgggc
    10981 attgccggcc ccggcggcgg cacgccggac cagcccgtcg gcctggtcta tacgggtatc
    11041 agcggtccct gggggaccgt gacccaccgc aacgtctatc acggcggccg ggtcgaagtc
    11101 aaacagcggg ctgccctgcg ggccgtctat tacttagtcc actatatagt gacacataca
    11161 aagaaagcat agttaggagg agacaatttg gacgcaaaac agacagcact tgaaaatgca
    11221 atgcgcaaga ttgaaaagga tttcggcaaa ggcgccatca tgcgcctcgg cgacgtcgcc
    11281 gacaagatga acctggaagt catctcatcg ggttccctgg ccgtcgacct ggccgtcggc
    11341 gtcggcggtt atccccgcgg ccgcgtcatc gaaatctacg gtcctgaatc gtcaggcaag
    11401 acgaccctgg ccctgcatgc catcgcctcg gcccagaaga gcggcggcat tgccgctttc
    11461 atcgatgccg aacacgccct ggatccggtc tatgcccgcc acctcggcgt cgacacgaat
    11521 gaactgctca tttcccagcc cgataatggc gaacaggcct tggaaatcac ggaagaactc
    11581 gtccgcagcg gcgccgtcga catcatcgtc gtcgactccg tagccgccct ggtcccgaaa
    11641 gcggaaatcg aaggggaaat gggcgattcc cacgtcggtc tccaggcccg cctcatgagc
    11701 caggccctgc gcaaactgac gggcatcatt tccaagtcca aaaccatcgt catcttcatc
    11761 aaccagctcc gcgaaaaagt cggcgtcatg ttcggcaacc cggaaacgac gactggcggc
    11821 cgggccctga aattctacgc ttcaatccgc atggaaatcc gcaagggcga agccatcaag
    11881 tccggcggcg atgtcatcgg caaccgggcg cgggtcaaag tcgtcaagaa taaagtggct
    11941 ccacctttca agcactgcga attcgacatc atgtacggca cgggcatttc ccgcgaaggc
    12001 acggtcctcg acctgggtac atctatggat atcatcgaaa agagcggcac ctggtattcc
    12061 tataagggtg aacgcctggg ccaggggaag gaaaacgtca agattttcat gaaggaacat
    12121 ccggaaatca cggacgaagt ggaaaagatc atccgcgata ccctggcagc agaaccggaa
    12181 aaattcgacg aagccatgga agaagaagat acggcgtccc tggatgcggc ggatacaact
    12241 gctgaaaaca gcgacgaagc ctaaatggat tatcgcgaag cttatgaaaa agccttgcag
    12301 ctcctcaacg tccgctttct cagcgaaggg gagctgaagg ccaagctgcg ccgccgcggt
    12361 gttgacgacg acgtcctcaa ggacgtcgtg gctgctctgg aaggggaaca cttcctcgac
    12421 gaccagcgcc tggcccgtga cgtgtaccgc tattatgccc gcaaagggca gtacggccac
    12481 ttgtatatcg tcaaccgcct gcgccgccgc tgcctgcccg taccggaaga cgtcgagcgg
    12541 cccgatgaat acgccctggc cgggaacctg gtgaagcgca agttctctta tcgtgccgat
    12601 gaagcccgca aggcggcccg ttttttacag taccgcggct tttcgccctc agtcatacgg
    12661 gacgtcctgg aagattttac ggtccattga tacgttaatc ttgactaagt ctatcttgac
    12721 attgatataa aaaaaattta caattaagag agccggtttg atacactggt ctatattttg
    12781 cataattgta ggttttttga acatgaagta aattttacat actcataaaa aaagccggac
    12841 tgcgagttcg gctttttttt gtgaaagggg aggtgaatcc attactatgc taacgattat
    12901 tgctgcagca gtcgcctgcg gtctcatcgg cggcggcacg ggctatattt accgtaagaa
    12961 gatcgccgaa ggcaagatcg gccaggccga agtcgaagca cagcgcatca ttgccacggc
    13021 ccagcagaac gcagaagccc gtaagaagga actcgtcctg gaagggaagg aagaagtcca
    13081 caagatgcgc aacgacgcgg aacaggaaca caaggaacgc cgcaatgaat tgcagcgttt
    13141 tgaacggcgc ctgttgcaga aagaagaaca tttggacaag aaatctgatt ccatggaaaa
    13201 gaaggaagat gccctgcagc gtaaggaaga tgaagtgacg cggatgcagg aaaaaatcga
    13261 agcccttcat gaacagaaga tggctgaact ggaacggatt tccggcttga catatgaaga
    13321 tgccaaggaa ttgctcctga acaacgtcca gaccgaagtg aagcacgaaa cggcgcagat
    13381 gatcaagaac ctggaagacc aggccaagga agaagccgaa ggcaaggccc gggaaattat
    13441 ttccatggcc atccagcgct gtgctgctga ccacgtcgcc gaaacgacag tatctgtcgt
    13501 ttccctgccg aacgatgaaa tgaaaggccg catcatcggc cgtgaaggac gcaatatccg
    13561 ggctattgaa acgctgaccg gcgtcgatct catcatcgac gatacgccgg aagcggtcat
    13621 cttgtcgtgc tttgatccca tacgccgtga aatcgcccgc attgctctgg aaaaactgat
    13681 tgccgatggc cgtatccatc cggcacgcat tgaagaaatg gtcgaaaagg cccgcaaaga
    13741 agtcaatcag aagatccgcg aagctggcga acaggcgacg tatgaaacgg gcgttcacgg
    13801 tctgcatcct gaactcatca aaatcctcgg ccgcctgcgt tatcgtacca gttatggcca
    13861 gaatgtcctg cgccattcca tcgaagtctc tcagctggcc ggcatcatgg ccgctgaact
    13921 gggcgtcgat gaaaacctgg ccaaacgggc cgggctcatc cacgatatcg gcaaggccct
    13981 ggaccacgaa ctggaaggga ctcacgtttc gatcggtacg gaaatcgcca agaaatacgg
    14041 cgaatccaag ctggtcgtca actgcatcgc tgcccatcac ggcgatgaag aagcgcacag
    14101 cgtcgaagcc gtcctggtgn nnnnnnnnna tgccgtatcg gctgcccgtc cgggtgcccg
    14161 ccgggaaacg ctggaaaact atctgaaacg cctgacgaaa ctcgaagaaa tcgccgaatc
    14221 tttcgatggc gtcgaagact gcttcgccat ccaggccggc cgcgaaatcc gcgtcatggt
    14281 caagccggac aagctggatg aatcggactg tgtcctgctg gtccacgaca ttgtcaaacg
    14341 catcgaagcg gaactggaat acccaggtca gatcaaagtc gtcgtcatcc gcgaaacgcg
    14401 gatggtggat tatgcaaaat aacctttttt gaattcatca ggacgcccgg tgatggcttc
    14461 ggccatgacg ggcgtctttt tcattcctac aaaaaaagtt tgaaaaaaat aaaaaaaagt
    14521 acttgccaaa acatccttct tgtagtagaa tagtttttgt tcgaggcact aagaaatacc
    14581 gaaacaacct cgaaagaacg aaagaaagtg gccccgtggt gtagcggttt aacatgtcgc
    14641 cctgtcacgg cgaagatcgt cagt
//
LOCUS       DAWABZ010000038        14484 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_1144_length_14484_cov_3.231340, whole genome shotgun sequence.
ACCESSION   DAWABZ010000038 DAWABZ010000000
VERSION     DAWABZ010000038.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 14484)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14484)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14484
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1144_length_14484_cov_3.231340"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4784..4793
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2936 a         4078 c         4238 g         3222 t
ORIGIN      
        1 cgacaagcct ttttaagtgg ttagtggcta gtggttagcg gatggtttta aatttaaacc
       61 atcttctaca aactccgcac tacaaactat aaagccaaag aacctctccg gcccgttggg
      121 ccacctcccc tatgagggga ggctttgaga accactattt tttatccttc ttctggagtt
      181 tggccaggcg gtcttttaag gacatgaact cgccctggtt gtcggactgg agttccgttt
      241 cgggctggat ggtctggtct ttataggcgg ctttgcgttt ggctgcttcg gcagcggctg
      301 cggcctgacg ttcttttgca gtccgttcgc tctggacggc ttcgcggatg ggctggagtt
      361 tattgtccgg gacgacgagg aagcggaccg gcaggttggc ggctcccggc ggcatgatgt
      421 ggatggtcat gtccgtcccg gccgggaagg tatcgacgta ttccatggca tacgggtcat
      481 tgaggcccat ggagtggcga tggcgcggca gttcgacgat tttcttgtct tcgccccgct
      541 ggggatcgct gtagatgagt tccgtcacgc cggcatattc gccgccctgg ggattgaaat
      601 acaagtggat gttcccggtc cccgtcgtgc gcaggtggat ggtgtagtcg acgccgtagt
      661 tgcccgtatc ttccgaggcc cgattgtcca tgacgtcacg gccctggagg aaacggtccc
      721 agacgccgtc agccaggagg atatagccga tgccgctgtc cgtcgaatag ggaatgagcg
      781 tgctcaggta acggtccttg ccgtggaaag tgccgcgcag ctggacggag tcactggcca
      841 ggtactgctg cttgcggatg aaggcaatcg ggtcttcctg gggcggcagg atcatggacg
      901 acaggatcat gggttccggc aggctgacgt tgacgatgcc cgaaaagagc tggtccggcc
      961 ggacgacagt ttcattgagg cgctgaccga cgatggcata gtcatgggcc gggacggtga
     1021 tcttattaac cgtcatgttg ccttcataat acatggtcga cagttccttg ccgacctggt
     1081 agtagtccgt actgggccgc gtatactgat aaccccggag gaccgcttcc agcggatacg
     1141 ggtgcggatt gtagagcatg gctacgattt tccggttata ctggtgctgg ttgacatgat
     1201 agaaatagag gcgcacgtcg ccgctgaccg tatccccata gaggatcccg tcaccatcgg
     1261 cgtattccgg gctatcagag aggagcagcg tccccgtatc gacaggagct gtcatggaaa
     1321 taggaagcat acgctgggcg acgacgcggt ccatgacgtc cgggtcgtcg acagcgggca
     1381 gatccgctgc cagggcgccg gccaggggca gacaggccgt cagcaggccg gctatatagg
     1441 tacatagttt cttaaatttc atcttctcgt tgaccttcta tctgtttcgc tatatcagcg
     1501 atttttttaa aacggtggga caagcccgat ttcgtcaatc ccgactgggc cgccagttca
     1561 ctcatggaag cttcaggatt ggccatgcgc aattccgccg cttcccgcag cttcggcgac
     1621 atgcgctggt acaggcccga ttcctggatg caggtaatgg cccggcgctg gcgcacggcg
     1681 gcgtctacgg tcttctggag attggccgtt tcacagttga cgacgcggtt gacattgttg
     1741 cgcatcccct tgacgacgcg gacgctctca aacttgagat acgcctgggc tgcgccgatg
     1801 tattgcagga attgggacac gccattgcct tctttgatgt aaaccaaata atcgcccttg
     1861 cggtccgtcg tcttggccgg caggcggaaa cggcgcatga ttttcaacaa tttttcggca
     1921 aaggccgcat tgcccgtcac cagttccagg tgataatcgc ccttgggctg attgacggac
     1981 ccgccgccca tgaaggctcc ccgcaggaaa gcccgctggc agtcggtctt cttgaggtcg
     2041 atggccgaaa attccgtatc tgtcggaaac agggacaaat ccttcaggat ggccgtgctt
     2101 tccggcgacg gcgatacgcg caggatatag taattcttct tgcgcaggcg caggccctgg
     2161 cggacccgga cttcgggccg gaccgggaac gattccatcc acatcttcag ggccttgcgg
     2221 gcgacggcat tattggacgt gctgaattcg atgccgatag tcccgcgcga gccgagtatg
     2281 agggagccgc ccatgcagag cagcgctgcc agttcggcaa aacggcaggc ggcattttcg
     2341 ggcttgcagt gggcgatttc attcttcact tcttctgcaa atgacaaagg gcgccgcctc
     2401 ctaacgatgg ttgaatgtgt aacgcttcaa atagtaatgc aagagctccg gcgacaaatc
     2461 ggtccgcaag gcgttgatca gatcaaagag gacgttggcc agcttcttcg tatcgtgagt
     2521 ggcactgttt tccttgttga tcaaattcga caggatgagc gtcgccccgg tctggctgac
     2581 ttccttcttg tcgatcatga ccggatggga cccgtgggcg acatagcgct ggaggacggc
     2641 cggatcgaca tcgccgttgt tggcgatgac gaagtcgatg accttgccgc cggcctggcg
     2701 gttgatggcc cggatgtggt cggatacggt gtaatcatcc gtttccccgg gctgggtcat
     2761 gacgttgcag atatagatct tcggcgcctt gctggtgcgg acggcctgga cgatatcggg
     2821 tacgcagaga ttgggcatga tgctcgtata gaggctgccc ggtccgagga cgatggcgtc
     2881 ggcttccctg atggcgtcga cggccgactg gatggccctg gggtgttccg gcgtcgtaaa
     2941 gacgcgcttg atcttgccgt gggcatgggg aatctgcgat tccccttcga cgatgcggcc
     3001 gtcggtcatt tcggcattga ggcgaagctt ttccgccgac gatgggatga ccttgccgcg
     3061 gacgcgcagg accttgctgg tcgcgtccat ggcttcctcg acgtcgccca ggacttcgat
     3121 gagggcggcg atgaacaagt tgccgaagct gtggcccgtc aggttgccgg acccgccgaa
     3181 gcggtgggcg aagagctttt ccatgagccc ttccttatcg gccagggcga ccaggcagtt
     3241 gcgcaggtcc cctggggcga tgatatccag gtcctggcgg atgcggcctg tcgaaccgcc
     3301 atcgtcggcg acggtgacga cagccgtcag gttatatgtc ttggccttga ggccgcgcag
     3361 catgaccgac aggcccgtgc cgccgccgat gacgacgacc ttcggccctt tggacagctg
     3421 gaggttggaa aagatcaggt cgctgaggtt atccgattcg ccgggcatga cgacgccgat
     3481 catggtacgg atcaggcgcc gcgtcgccca ggccatgacg accaacccca ggaggatgac
     3541 caggatgccc atactggcca ggatggtata gttataatgg cccgtcactt catagagcag
     3601 gcggaaagcc cattcttcca gcgaccccag ccattggtag ttaaaggtca ggataatgcc
     3661 gaagctggag cacatcatgc ccaggccgaa gagaaagagc catcgcttga tgtgcaagcc
     3721 cggatgcagc catttcataa aacgcatgac agcacctcct acgcttcgcc ttcgtacccc
     3781 ggcgcatctt cttcgacgtc gttcttctgg atatcccggt gttcgatgga tacgtcgaca
     3841 ccgttatccc tgagatgttt atacatggct tcggccatac agacactgcg gtgcatcccg
     3901 cccgtacagc cgacggcgat gaccagctgg ctcttgcctt cccgttcata gttgggcagg
     3961 aggaagtcga tcatatcgaa ccacttttcc ttatacgtct gggtaatggg gaagctttcg
     4021 acatagtcgc gcacacaggg tacacggccc gatttatgtc ggtattcttc aatataataa
     4081 ggattgggca ggaagcgcac atcttcgacg atgtccgcat cgatgggcat gccgtgcttg
     4141 aagccgaagg acacgacggt aacggtcatg ctcttcttgc tgtccgtaat ggcatagcgc
     4201 ttgaccagga tctccttgag ctgcttcggc ttgagattcg acgtgtcgat gatgaaatcc
     4261 gctttctggc gcagcgaggc caggcgcttg cgttcttcgg cgatgccctg gctgatgcgg
     4321 ccatgcgggg ccaggggatg gacgcggcgc gtttccttat agcgccgtac cagggcctga
     4381 tccgaagcct caatgaagac gatttcgtag gaaatgcctt cggccttgag gtctttcagg
     4441 cactggctca tggcgtcaaa gaattcaccg ccccggatat cggcgatgag ggccacgtgg
     4501 cggacccgtt cgccgccttt gatgcacaat tccgcgaact tgggaatcag gaccggcggg
     4561 atattgtcga tgcagaaata gccgatgtct tccaatacct ggacagcatg gctcttaccg
     4621 gcaccggaca tgccggtgac aatgatcaaa tgaaaatggt ccataagaac ctcctatata
     4681 gacaatgtta caatacaaat ttctcaaccg ccgcggcgac gccgtcgtca ttgttggacg
     4741 ccgtgacgta agacgcgacg gcctttactt cgtcggcagc attnnnnnnn nnncaaatcg
     4801 ttctgggaat cgccgagggc catgatctca tcggccgtga tgccgtagag ctcgcccagg
     4861 cggcggatac ccttgccctt gtcgacgccg ctgggcatga tttccaggaa tgtcggcatg
     4921 gaaaagagga tgttgaactg accggggaaa cgctcctcga tgagggcctt gcgggccacc
     4981 aggtcgtcgt gctcgccccg gctgagaagc ttgttgcagt cgccctggac gcggtagaat
     5041 tcgtcgccgc agatacaggc cttgctgtgc gtgatttctt catagccgcg gatccactta
     5101 tcatccttgg ccgtgcgcag gacgtcgtcg atataggtct gcatctgcca gcccttttcc
     5161 cgggccaggt cgaccagttc ctgtgccgtc tgctgcggaa tggcctgctg gaagagaatc
     5221 ttcttcgatt ccagcgtctc gatgaggccg ccggcaaaga gcagcagcgg actgtcgccg
     5281 aggtccaggc ggtcgccgta aggccgggcc gtctggtaca tgcgtcccgt ggcgatggcg
     5341 acgacgacgc ccttatcgcg ggctgcctgc aaggtgcgga ccgtccggtc cgacacgctg
     5401 ccgtcgctgc gcaggagcgt cccatcgagg tcgatggtga tcatctttac cggcttcatc
     5461 aggccttgcc ccccttccag tcgaagacga cgccctgact ggacggttcg gcttccttcg
     5521 gcgcgtcgct gacgtactgc aggtcgtcgg catagctctg ggccagttta tcgatttccc
     5581 gctgctggtc tgccgtggct ttgctgcggt tcttcatggc agcggcgatc atttccgtca
     5641 cttcatagcg cgtcaggttg tacgacggcg caaagcgcgt catcggcacc cccgtcaggc
     5701 cggccttgga caggtcgtag acggccttgt attcccaggt attgcggtct acggcgtcaa
     5761 actggttcgg cgcataggga ttataggcca gggccgcgct gagccccagg cagcaccagg
     5821 ctgccagcaa aagaaagaat cgtttcatcg ttgtccccct ctataaacaa atatactccc
     5881 tgccgggtct aacgtgcgtc atacctgaaa gagagcatat ccataaaata cgtattattg
     5941 ggctttttta gcgtcctttt tggcatcact gtcatcgtgg ctgccatgat cgggtacggc
     6001 tttctgcgcg gcttcggtcg aaacggcgac agccggggct gccggtttga cggcgaccga
     6061 tttatccgca gcgacaggtg ccgcaccttc gccatcgagg gccatgtcgt cgagcagctt
     6121 gagctggctg gtcaggatgg cacggcactt ggcgcggtat gccccggtct gggccttgag
     6181 cttttcccat tcctgccggg actgctgcag attcatcatc gtttcgtcga gcatctgctt
     6241 cttcttgctt tccgcttcct gcaggatgag gtcggcttcc ttgcgggccg agaccttgac
     6301 gttttccgcc gtctgctggg ccagcatgag cgtactgttc atggtagctt ccatcttttc
     6361 atactgcgtc agcttgtcct gcaggctgtc gcagcgttcc ttcaattcgc agtattcccg
     6421 gtataccttt tcgtagtcgc cggcgatgtt gttcatgaaa gcatcgacgt cttcttcgct
     6481 gtacccgcgg aagccgcgtt taaattcttt gttgtgaata tccattggtg taagcatggt
     6541 atttcctccg tgtgaaaggg aattacatat accgcttcag caagacgccg atacggccct
     6601 tcctggacgt acccgtgatc tcggccagtt ccatccggcc cttgccgcgc agcgaaatga
     6661 catcgccctg actgacgtcc tggctcggtc ccttggcagg ctgccagttg acctggaccc
     6721 ggtcgccctt gatggcttcg gccgccttgg tacgggaaat gccaaagccc gaagatgcaa
     6781 tggcatcgag gcggagcgat gcgacggtcg tcttgatttc cttgactttt tcctggcgag
     6841 gcgcaatgtc cgacaggggc atctcttcga tggcaatgga taccatggca attttagtga
     6901 aattctgctg taaatacggg acgagtttcg tatcggccaa gaggacggcg ccggcatcct
     6961 gcatgatgat gtcgccgaag cgttcgcggc cgacgccgag gctcatgagc gagcccagga
     7021 cgtcgcggtg ccccaggagc cggtagcggt catcccagga caggcggcag gcggtgatgc
     7081 cgaaatcgac aggcccgtcg taatcggcgc tgacgaaagc cagcttgacc cgttctgccc
     7141 cttcatagcc gccaaaggtc ttgaggacca gggacggcac gtgggcctgg atggtctcgc
     7201 cgatctggac ggcaccggga gatacgaact cactgaccgc ataagggcgg cccttggccg
     7261 tgctgtcggc caagtcgata aggcgggcgg cgagcttgcc attatcggtt ccctgataat
     7321 atcggatgat ccgatctctg ttcttcaaac tgatccctca attactagtg ctgcggcgtg
     7381 ctgctgccgt tgttgttatt gttgttgctg ttccaggcca ggggcggcgt attgaaatcg
     7441 ccgtagtcgt cacgcttgcc gtggtcgacg gtgacattgc tcggtacgca gaggaagatt
     7501 tccttgccga ccttctggat gttgccgtcc agggcatacg tagcgccgct gacgaaatcg
     7561 acgacgcgtt tggcaatgtc gtccggcgtc gattcaaagt tgatgacgac gggtttgcgg
     7621 tcgcggatat aatcggcgat gctctgggaa tcgtcaaagg tttccggttc gacgatgacc
     7681 atgcgcagcg gtttttcctg gggtgctgca taattgacgc cggaacggcg cggggctaca
     7741 gccggcgtgc ggcgcggtgc gcgttcttct tccatttctt cttcatcctc gtattcgtct
     7801 gtttcaaatt cgtcgtcttc gttgacgccg cctacagtac ctactaattt atcccaaact
     7861 ttctttactc ccatggtgaa tactcctcct tagtaattac ggggcccaaa gattgccgta
     7921 cctacgcgga cgacattggc gccttcttcg attgctattt caaaatcatg ggtcatgccc
     7981 atggacaggt aacggatttg accttccgga aacttcggct tcatgtcttc atacaaagcg
     8041 tgggcaatgc ggaatacagg ccgtacatcc tctacgttgt cgtaattcgg ggcgatgcac
     8101 atgaggccca ggacgcggac gtgttccatg gtcttggcca tttcgcacat agccgggaag
     8161 tcttccactt ccatgccgaa tttgctttct tcgcgggcca cattgacctg caggagaata
     8221 tcctgtactt tgccgatctt gccggcttcc ttatccacag ctttgagcag gtgttcggaa
     8281 tcgatcgagt ggatgaggtc gaagtgttcg acggcatgtt tggccttatt ggtctgcaaa
     8341 tgaccgatta agtgccaggt caaatggtta tccgggaacg cttccagctt ctgaagtgct
     8401 tcctggaccc gattttcacc gacgtcggtg acaccgcatt tagcggcttc ttccatcatt
     8461 tctacgggat gattcttcgt gactgcgaca agcgtaattt tgtcagtcct gccgacgcgg
     8521 gcagctgcat cggcaattct cttatttact gcctcaagat tttccttaat caagcctatc
     8581 cctccgtagt gcatgtgatt atatatattc atatatttat aaaacattac acttctttta
     8641 ttatatatag ttaaggctat aaaagctagt catatttaaa aatttgtccc atctgctttt
     8701 caaaggacag caaaggcccc catgcggccc gtatgaccgc cgtcgcggcg ataggaaaag
     8761 aacttccctt cctcggcaaa ggcacagctc gtcgtgactt cgatgtggtc cggccgaagg
     8821 cccgcttcct ccaggagcag ccggttggcc tgccacaagt caacggacca gacgccatct
     8881 ttttcgccgg cgcagttccc ataagccgtc cccaggcggc ggaaggccat ggccgtagac
     8941 tcgctgacct cgtaatgggc ccgggaaatg gaaggcccca cataggccag gaggtctttc
     9001 gggtccgacc cgtaggccag gcgcatggca aagaccgtct tggccaccag gcgctggacc
     9061 gtaccgcgcc agccgtcgtg gatgacggcg acggcccgct tcaccggatc gtagacgatg
     9121 accggcacgc agtcggcgat gcagagcatg agcggcacgc cgggaagatt ggtcatgatg
     9181 gcgtccgtat gggccagggc atcggcataa ctgccggcac cgcagccgat ctctgccggt
     9241 cccacggcga cgacgtggtc ttcgtgggtc tgctgggccg tcgtcagctt ctgcagggga
     9301 tagcccagga gggcacagag gcggcgccgg ttctccagga cggccttggg ctggtcgccg
     9361 acgtggagcc ccaggttcag cgacgtgaac ggggcctcgc tgacgccgcc gctcttgccg
     9421 gtcacgccat ggcgcaggcc ggcgtctgcc aggagggtgc tctcttccca acagattcca
     9481 ttaccgtgtt ctacccactt agtcatgata tcctccacaa atgtgacagc gtcggcagcc
     9541 gtcgaaacag gggatggtga attccccgtc tttggctttt ttcagctcgt ccgtcaggta
     9601 cgtcggcacc agtcccgtgt cgatcgtatc ccagggcatg acggcgtctg tcggccgctg
     9661 gtcatataaa aatcctttaa tatcgaaatg aagctccttg gccgcccgtt tccagccctt
     9721 gacgccgccg tattcatggg ccttgaccag gaggtcgccg acgcggcggt cgccccggga
     9781 caggacgccc tggatatagg ccgagcgcag gggctcgatg agcagctcga tctgcttgtc
     9841 cttcaaggcc ttcttgagga aattcagccg tttctcgacg accttctttt ccgtcatggc
     9901 catccactgg aaaggcgtga agggcttggg gatgaagggg ttgatgctca gggtgatgcg
     9961 gctcaggttg ccgatggccg ccatgtgctg ctggacgcgg cgggtcatat cgacgatgcc
    10021 ctggatatca tcatcggttt ccatgggcag gccgaccatg atgtacatgc gcacgtggcg
    10081 gataccggct gccgtcgcca ggtcgatgga atggagcagg tgctcttccg tgatgccctt
    10141 gttgatgatg tcccgcatgt gcttgctgcc cgcttccggc gccagggtaa tcgtcttctg
    10201 gccgctttcg gccaggccct tgacgatggc cggcgtaatc gagtcggccc gcagggacgc
    10261 acaggaaaag gccagcccct ggtcgcggat gaacgtgacc agttcgtcga tatagggata
    10321 atcggaaatg gccgcgccca tgaggccgat tttcttgccc agggccttgc cgcgcaggac
    10381 ggcggacttg acgtattcca gcggacggac gcggggacgg cggtaacagt agccggccat
    10441 gcagaagcgg cagtgacggc cgcagccccg ggcaatctcg atgaggtaca tggcgccgaa
    10501 ctccgtattc ggcgtggcga tgaccgtttc cgccggctgg gtcagctcgt gccattggcg
    10561 ttcgaccttg gccgggatgc cctcttccgg aacgatgcgc acgacgtggc cgtcgtcatc
    10621 gtattcgtgg cggtacaggg acggcacata gacgcctggg acatgggtag ccagatagcg
    10681 cagcaggtcg tgacgggaca ggccctgggc ctttccttct ccataggcgt cgaggaaggt
    10741 atggatgaat cgctcccctt cgccgaccag gacgacgtcg acaaaaggcg acagcggttc
    10801 ggcgttgaaa aaggcgacag gcccgcccat gacgacgagc gtatcgtcat catcgcggtc
    10861 agcggccagg acggggatgc gccccatttc caggatcgtc ggcacgttga aatagtccat
    10921 ttcaaaactg accgacagac cgaggatttc gaaatccgac aggggccggc ggttctccag
    10981 ggaaatgagc ggttcccctg tcttctcgaa gaccttcatg aggtccttat ccggcaggaa
    11041 agcccgttcg cagagccagt cctcgcggcc gttcacttcg cggtagatga tctgcatccc
    11101 cagattgctc atggccacgt catacgtatt gggataacag atagccatgg catgacgcgt
    11161 gccgaaagga tactgtacag cccccgtttc ctgggccatc agatcatcca gcgctttctc
    11221 tacttgccag ctcatgcatc cccctccttt cattcatcgt tcgttaggta tcctatattg
    11281 tatcacagaa tatcccgatg ggccagccgt gtcttgcatc gccgctccca tcgtgttatg
    11341 gtattaattg atgttcgcag gccgcaggcc gcaaagcctc ccctcatagg ggaggtggcc
    11401 caacgggccg gagaggttga attaccgcag gccgcgttgc aaaagccacc tctcagtcag
    11461 ctgcgctgac agctctccta tgaggcgagc cttttgaaag accccctcag gccgccgcgg
    11521 tcccacggac cgcttccgta ggggccgtcc caaagggcgg cccgcttatt tcataaaaaa
    11581 aggagtccta ttcatggaaa aagtcctctt cgtcgccctc ggcggcggca tcggcgcagc
    11641 cctgcgctat ttactcacag aactggccaa cctccacggc ggcgtattct tcccatacgg
    11701 caccatcctg gccaacctca tcgggtcctt ctgcatcggc atcctcttcg tctttttctc
    11761 cgaaagcagc gacctgtcgc ccatcgtcaa actcttcttc gtcacaggta tcctgggcgg
    11821 cttcacgacc ttttcgacgt acaacatgga actcctcacc ttcgtccgta ctggcgccgt
    11881 cttcccagcc ctggcctact tctcgttcaa cgtcatcggc ggcttcgtct gctgctggct
    11941 tggcttcgcc accggcaacg ccttgtgggg atgagagaag ggccgccaga gcggcccgca
    12001 ggtcgcaggt cgcaggtcgc aagcctcccc tcatagggga ggtggcccaa tgggccggag
    12061 aggttgaatt accacatact acttcaaaag caacctctca gtcagctgcg ctgacagctc
    12121 tcctatgagg cgagccttgg gaaagccccg caggccgtaa agcctcccct cataggggag
    12181 gtggcccaat gggccggaga ggttgaattt ccacacggtg atttaaaaac aacctctcaa
    12241 gggagcaaaa aaagaggctg tcgcattaag acagcctctt tttaagtttg tagtttttag
    12301 tttaatcgcc gtatcggccg accggttgcg tttgcgctaa gatggctgga tatgcgcgct
    12361 ggggcaaggc ggaggaagga ggcgtagcca tagctacgtc gactgacgac aacgcagtcc
    12421 cagcgtgcag agccggtcag atttgcgcaa taagcagacg gccggccgat actcctaatg
    12481 ctgtgccggt gcgccgacag gtttcgtttc ttctacgagt ccgtccgttg cgatggcttt
    12541 ctgggatacg aagctgatgc cgcagaaggt gacggcgcag acaatccatt cggcataaat
    12601 gacgtgcggc agtacgcgga cagccggtac gaacatccag gcgacgagga cgatgatgga
    12661 agccaggatg gtgtagaacg cagcctgacg ggagcagtat ttcggcgcat acatggtcat
    12721 gaggacgatg actgcgaaag ccgtcatgag cgacaagccg gccatgaggg tcgaaatgat
    12781 gccgctgatg gtcagggcga agcccaaggt caacaggccc aggagcagga cggaaatacg
    12841 ggttacggtc atgtatttct tatcggacat attggggtcg atgaactgtt tatgaatatc
    12901 atgggaatac agcgtcgcag ccgacagcag gagattgcaa gccgtgctga catcagcagc
    12961 ccacagcgaa gccagtgtca cgccggcaat ccagggattg agggacatga tgagcttcgg
    13021 cagtgccgtc gtcggcgaaa tagccggata catttcggca gcgatgacac cgaggagagc
    13081 ggcgatgaaa ccgacaggca gcatgaccag accaccgatg atgaaacctt tgcgggccgt
    13141 ctggacatcc ttggcaccga gagaaatctg aatgatagcc tggagcgata cattgaccgt
    13201 aatgaggacg acgatccaag tgatgatggt catgggaccg acaccgctga tgaggtccat
    13261 ggacgtaatg gacggagcct ggatagcgac gacgtcgatg ccgccggcca tgcccaggat
    13321 gaggaaagca gcaaccgtaa tgccgatgta ctgcaaggta acattgagga cgttagactg
    13381 gctggccgac cacataccac cgataagagt aatgccgatg aagacgacgg cactggtgag
    13441 catccctgtg accggcgtga agatattcgg catcagggca gccaggatag cgccgcctgc
    13501 gacgtactgc aggctcatga tgaccagctg gaccaggacc tgacagccga taccggcgat
    13561 catgctgcgg cggtcataat accgttccag cagttccggt acggtcgtaa tattgagctg
    13621 gcgatacttc ttggcgaccg tcaggcccat gacaatggca ccgatgcccc aggcagccgt
    13681 ataccagccg gccgacaacc cgactttata agcctgttcg gcaacgccga tggtcgaagc
    13741 cccgccgaca gccaagccga cgatggacac catgatgagg ggcgtcgtca atttgcggcc
    13801 agccaacaca tacgcttctg ccgatgcggc agccttatgc ttgacgtacc agctgatacc
    13861 gaagagaacg ccgatgtaca ctaaaatgat gaagacttga atccctgtag ttcccatagt
    13921 aaaaactcct ctctaaaata atttcccgaa aaaaaagccg cccctcacaa aagggacgac
    13981 tcagtcgcgg taccacccaa tttatcgata tatggataaa aaaaagagtg gtacgccagg
    14041 gacggaagat tccgcggtac caccctaatt atctcgaatc gatcaactca tggccgtata
    14101 acggcagccg ccccggtaca gcctactcac gttcagccgt acagttcagg aatgaacttc
    14161 atgccctctc ccctgtcccc tcacaccggc cggagactct ctgaaaagct caaagacact
    14221 acttttttcc atcacaactg ttatcaggaa atctgttgtt gaccatgatt atacaggaaa
    14281 ataaaataaa gtcaagcaaa atctgacgaa aaaatcgaaa gaaaatcacg taaaacgtac
    14341 tacgaattag gtaaaacgtg cgacctgaga atcaacacaa tctctgtgtt tttatgcgac
    14401 ttatagtgat agcgaaataa ttttcccaac actggcaatg acgccagaat cggcaccttg
    14461 cggaaatgct cgacctcttc ccgg
//
LOCUS       DAWABZ010000039        14127 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_1182_length_14127_cov_3.480529, whole genome shotgun sequence.
ACCESSION   DAWABZ010000039 DAWABZ010000000
VERSION     DAWABZ010000039.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 14127)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14127)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14127
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1182_length_14127_cov_3.480529"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3301 a         3729 c         4051 g         3046 t
ORIGIN      
        1 actaagagga gaatgaatgt gaagttgttt tctgattttt atgctttggc agcggcttcg
       61 cccaattctt tgcattcagg agcgttgtcc ggtgtttcgt tgacgatggc cgtaccaatg
      121 acggtggcac cggcatcttc tgtacgctgt ttccatgcgt ccatccattc gccggaaccc
      181 cagccgtaag aaccaaagag gccgactttt ttgcccttca atttcggagc caaatcggtg
      241 aagaacggtt cgacgacaga gtcttccagt tcttctgaac ccatggccgg gcagccgagc
      301 aagatgacgt ctttgctagc tacatcatcg acatttgtat cttcaaagcg gactgattct
      361 acgtcagcac cggcagcttt gacggctgct tcgatttcgt tagccattgc ttctgtatta
      421 cctgtaccac tccaataaac gatttctacc atgattaaat cctccttatg ctacaatgag
      481 cgagtctaat gttgtcccaa cggcaaagcg attcaaatag tcttgccggc agcacgtata
      541 gcgatggaaa gtacgggcgg cagcgtgggc gtcgccgtat accttccatt cgccacaaag
      601 cttggcatgg tttcccttgt tggcgatgaa accgcacacg tcctctaggg gatagccgag
      661 gaaaatgccc gattcatgag ggaaacagcc atcttctgca aagcgttcgg caagacggtc
      721 cagcagggaa gagatgtcct ggcagtcctg atagccgaat tgctgcagaa atgatgaaat
      781 gtcccgctgc tgtacgtagg cccagaccct ggccggtcgg tatacataca gcaaagtgcg
      841 tttggaacag gcgtacaaga tacggaaata caagcctttt gcattgtatt tatgattata
      901 attttcaatt atatcatcat aggtacagcc gctgtccaag cggggtagac agagaaggga
      961 tgcctgtttg agacccgcta aagtcggtga acagtgggtt attaagacga aatccaggtt
     1021 catatccggt tgccgctcct ttcgctttga tactgtaagt ataatttatt taaaagtgat
     1081 tgtcaatact aatatgataa ttcgtttcaa ataagcgaaa ggcttgccaa gtatagtata
     1141 ataaaggtag tgtgaagaat attcggaggg gggggattag atggaaactg cttatgatac
     1201 gaatcattgt ttcggctgtg gccgcgataa cccgattggc ctgaagctgg acatgaagat
     1261 ggaaggcgat cactgcgtgg cttattttac gcccaaggca gagcatgaaa gttatggtga
     1321 ccgcatgcac ggcggcctga cgagtacgct gctggatgaa gtcatgggag attatgtgtt
     1381 ccgcattgcc ggtaaaccag cctatacggc gcggctggaa atccgtttcc gcagtgccat
     1441 acgcatcggc gaaacggtca aggtcgaagg ctggccggaa gtccaccgcg gccgtctgtt
     1501 catcatgaaa ggaaagatta cccacgctga tggtacgccg gctgccgaag ccaaagcgga
     1561 aatgatgctg gctaaataaa ggaggagtta ctatggatag aagcgatatg atgcaccgca
     1621 tgtatgatat taaagggcct ttagtggcca agaagtttgc agcccgcggc tttgaagccg
     1681 tatactgtcc gacgaaggaa gaagccttga agcaggccat ctcgtatatc ccggaaacgg
     1741 atgtcgtatc ctggggcggc tctgtatcca tcaatgaaat cggcctgcgg ccttatgtac
     1801 tggaacacaa tccggtcatc gaccgcgatt cggccaagac gccggaagaa cgggtcgaac
     1861 tgatgcgcaa ggccctgctc tgtgacacct tcctcatggg ggccaacgca gccgtccagg
     1921 acggccagct catcaacatc gatggcaacg gcaaccgcgt cgccgccctt tgctacgggc
     1981 cgaaacaggt catcgccatc gtcggtatga acaaggtcgt cccgacggtc gaagccggca
     2041 tcgaacgggc gcacaccgtg gcgtcgccgc agaatatcgg ccgcttcccc ggtgtcggta
     2101 cgccttgtgc caagacgggc atgtgtggcc actgcatccg gccggacagc gtctgtgccc
     2161 aagtcgtccg gacgcgtatc tgcaagcccc agggccgcat caaggttatc ctggtcggag
     2221 aaaatctggg cttttagtcc tgaaaagaga ggtattttat tatgagaact ggaacgggcg
     2281 tcgtcgaaga agtctgcccg aatggcctgg ccaaaatcca gaccaaccgc aataatctat
     2341 attcgccgtg cagcgacagc atgtgcgcgg acaatgtcgt catcgatgct gtgaacccca
     2401 tcggggccaa aaagggacag tatgtacagt tcaacatccc cgacgccggc atgggcgtca
     2461 agggcatcat ctgcttcggc gtccccttgc tgctcatcat cgtctgtggc gtcgcaggct
     2521 acctgctggg agaatcatgg caggtcaaac cggaaatgac ggcctttgcc ggcatgattg
     2581 caggcggcat catcggctgc ctggtcctga aaggctatga aaagaaaatc aacaacaacc
     2641 agaccaaagc cgaaatcacg ggaatcctca gttaagaggc tgcaggttcg taggggctcg
     2701 cacgtggcga gcccgccaga tatatgtaag aattccgtga acaattggca tgagcgggcg
     2761 ccccgcgtgg cgcccctaca gaaaaaggct tgtcgcacgt gcgacaagcc tttttttata
     2821 caatatcgat ttggcaggct ttcatggcct gtaatgctct ttcgtggttt tctggtgtcg
     2881 aaccggcaca gcatgtgctg tcgacgatga tttccttttc cgggtagaag gctttgagca
     2941 gcatggcatt ggcgatgacg cagatgtccg tgcacaagcc catcaatacc agccggtcgt
     3001 agtcgcgtac ttcatcgacc agggccggac agccaaagga tggcttttcc aggatggtgg
     3061 cccacatggt gtacggcagg agctgtttgc agatgcgcca gccccgcgtg tctttcaggc
     3121 agtgtggaac gggaagtttc cggccttcct gggtttcgct gtaggcctcg tcgtgggtgt
     3181 cctgggtgaa aatcaggtcc gtttcctggt cggcatcctt tttgctggcc acgatcttgg
     3241 cctcgatgcg ggggacgacg gcttgagcct gagcgcttcc cagggcgccg tcgatgaaat
     3301 cattctgcat atcgatgatg atataagcct gggtcattac ggtcatccct tctctttagg
     3361 cacgcaattc ttttaaatat cccggtacgt ccgggcaggt catgaggccc gacatgaggg
     3421 cgacgccttt gatggggacg tcctggagct gagagatggt cttcggcgta acgccgccta
     3481 aggcatagac cggaatcttg acggccttgc agatggcttc gaccgtgtcg acgccgatag
     3541 gcggatccga cggcttgcag gctgtcggga agacgtggct ggccatgagg tacgtagcgc
     3601 cgagctgttc ggctttgacg gcttcttcga cggtgtgtac ggagacgccg agggtcgtca
     3661 tgtaatagag cagggacgaa tgggtttcca aataaggcag agagcactgg aaacggggaa
     3721 tatgaagacg gatagcagcc tttccgaaat gctggaggat acacgtcttc tgatggatgc
     3781 tgcacaggcg cagggcccgt ttcgcatagt ccgtgtattc ttcttcagaa aggttttttt
     3841 cacgaaggac gatgaaatct acatcggatg cggcgatttg ctcgagctga tcccagtagt
     3901 tcgtcgaagc caattcgtga tttgtaattg cagctacttt taacacgatt tatcacctga
     3961 atattagagt attttatata tataactata catgaatctc atactaacgt caacaattat
     4021 accaatagaa tataataaat gttctatatg agtttaacgc cctaaaacga gaagatatat
     4081 gacaaaaacg gttcggggtg gattgggtcc cggaccgttt ttgtcatata cattttatag
     4141 gattggtaat ggattacgtt agatggttta caaataaata tactcactgg tgacaggctg
     4201 caggcccatg cccttgatgt cggagtacat ctggtcaaag gaccggccgt cagtgatttc
     4261 gaactgttcg tcacccattt cggagccgtc attttccaga tggccgccga tgccgacgca
     4321 gacgctggcc gatactttgg tagcggcgat tttcatgatg gcgttgcggt agcgggcgct
     4381 ttcacggctc gatacgacga tgcaggcgta aggcatgaac agacggtagg cgcagatgat
     4441 ctgcgtcagt ttccgttcgt ctacgtcctt cgggttgatc ttatcgttat tgatgatcgg
     4501 gcacaggcgc ggacaggaca gggaaatttc ggcatgcggg tacttacgct gcatgagcca
     4561 tgcgtgcatc cccgttgcca gggcatcttt ctggtagtcg tccaggccta agagggctgc
     4621 gaagccgacg ccgcgcatgc cgccgaggat ggcccgttcc tgggagtaga agcggtacgg
     4681 gaagatgcgc ttgtgaccgg ccaggtgaag ggtagcgtac ttgtctgaat tgtacgtttc
     4741 ctggaagacc gtgacgtagt cggcgccaca ttcgtggaga tatttgtagt cttccgaatt
     4801 gaccgggtag atttccatgt tgatgacccg gaaatactgg ctggcgatct tgacggcgtt
     4861 gccgatgtat ttgatatcgc tcatcttcgg gctttcgccg gtgagcatga tgacttcttc
     4921 gatacccgta tcggcgatgt tcttacattc gcggtggagt tcttcgtccg tcaggcgggc
     4981 gcgcttgatc ttgttgtgcg agttgaagcc gcagtagatg cagtagttat cgcagtagtt
     5041 ggcgaaatag atcggcgtca ggatggtgat gttgttgccg aaatggcgcc gcgtgaccag
     5101 atgggctttc tgggccattt cttccaggaa cggtgcagcg gccgtcgata agagggccat
     5161 gaagtcccgc gggtccagat gttctttcga cagggccagg cggacgtctt tatcagtgaa
     5221 gtcgtcattg ttgaaggaat tgcggatggc gacgacctgg tcgagcatat ccgagtcgat
     5281 gacttccatg cccggtaaat atttcatgtg gtcgatgagt ttgctctgat cgatagtttt
     5341 agtttcttcc gtcatgttcg attcctcctc agtctcctaa gaatcccgtc agcggatccg
     5401 aagccgaagc cgtttcgcgg acgcggccca agccggcaag gtaagcattg cggccggcac
     5461 ggatggcttc accgaaggct ttggccatga gaggcaggtc gccagccgta gcgacggcgg
     5521 tgttggccat gatagcagcg gcgcccattt ccatggcttc gcaggcctgg gacggacggc
     5581 cgataccggc gtcgacaatg accggcaggt cgacttcgtc gatgaggatc tggatgaaat
     5641 ctttcgtcga caagcctttg ttggtgccga taggcgaagc cagaggcatg atggcagcag
     5701 cgccggcgtc agccagggca cgggcggcgt agaggtccgg atacatgtac ggcaggacga
     5761 cgaagccttc tttagccaaa atttccgtag ctttgatggt ttcgttgttg tccggcagga
     5821 gatatttgga gtcgcgcatg acttcaatct tgatgaaatc gccgcagccg acttcacggg
     5881 acaagtgggc gatgcggacg gcttcgtcgg cattgcgggc actggacgta ttggggagca
     5941 gggtgacatc tttcgggata tagtcgagga tattgtcttt ctggccggct tcgacgcggc
     6001 gcagggccat ggttaccatc tgggcaccgc cttgttcgac agcggccttg atgaggtcga
     6061 cagagaattt accggaacct aagatgaaac gcgatgtgaa ttcgtggccg ccaaggacca
     6121 gggtatcttt tttttccatt gggaaacact ccttttataa tgatagatga gttcatgact
     6181 gctttgccgc agggaatcag cccccgccga cgaagtgtac gatttccatg gaatcgccgt
     6241 ctttcaaagt cactttatcg taatccttgc gcgatacgat atcaccgttc agctcgatgg
     6301 cgatgcggtc tgtacgatat tcctggtcgg tcaggtaagc gagcaggttc tggccggccg
     6361 cttcgatgtc tttgccgttg atccgtacca tggaggtcct cctttctctc aaatgaaaaa
     6421 agggtcgcac gaagaattac acccaaggtg gtgtacccct tcgtgcgacc cttgtgattc
     6481 ggtctcactc tgcctatgat tatagcctat tgtcacaaaa agtcaaccgt tgataatgaa
     6541 ctgtcttgag agaaatgacc ggggaagacg acaaaggccg aaaaactata gtataataaa
     6601 gttatgattt tacgagaggt gtgatcactg ccatggaaga acggatggac tggatgaata
     6661 ttgcccgtta tttttatttg accgacgagc ggctgcagca gatttcccgc gattttgcag
     6721 acgacatgga acgggccctg cagggaaagc cgggagcgac cgtatcagcc ctgaagtcgt
     6781 atgtgtccct gcctggcggt gaggagcgcg gtgtctatct ggtcctggat ttcggcggta
     6841 cgaacgcaag ggctgcgcgg atccgcctgt tgggccggca ctgttacctc atcgaaaaga
     6901 aggtctgcca gccgctccgg ctgcctggcc agtacgacta tctctcttcg gcgacgacgg
     6961 ctggggagct tttcgatttc ctggcccgcc tcgtcggtca ggttgcccat gggcagcagg
     7021 cctataccct gggccatacc ttttcctttg ctatagagcg gcaggacctg cgcgatggcc
     7081 ggctcctgtc gtggtcgaaa gaaattgccg ttcccggtgt ggaaggcgaa tgggtcaacg
     7141 ccatgctcaa acaggccctg gcccgtcagg gctttagggc tgtcgaaccg gtggccctgg
     7201 tcaatgacac gacggccctg ctgttgtcgg cagcgtatac gctggaacgg gtccgcctgg
     7261 gcctcgtctg cgggacgggc ttcaacgcct gctactatga gccggcctgg gatatgatcg
     7321 tcaatctgga agccggcgac tatggcggcc tcatacgcaa tcgctgggac aaggcagtcg
     7381 acgccctgtc ggcccagccc ggtcagcatc ggatggaaaa gatgacaggc ggtgcctatg
     7441 cagcggaaat cttccggcag accttgctgt cttatttcaa gactcgccag ctcccggtgt
     7501 tttccacagc cgctatgaat gacatcatca gctgcgacaa tgatttgcag tgccggctca
     7561 agatgggcca gctgtggaag cgcatcgtgc ggcccgatga gatgcggcct gtccgcaata
     7621 tcggcgccgc cattttcgtc cgcgctgccc agctggccgg cgccgtgagc tgcggcatcc
     7681 tgcgccatct ttatggcggc gaagccgttc cggcccagtc cgtggccgtc gacggcagcc
     7741 tcttggaaca cgtccgcggt gccttgttca tgatggaaga tgccatgcag gcctgccaga
     7801 atgaaggcgt gagccgggac aagcagattc ccgtcgaccc ggtactcatc caggacggcc
     7861 ccttagtcgg cgccgccgtc gcagcggcca tggcaggcga aggccggtga cagcaggccg
     7921 gcccaccacg aaccacgagc cacgaaccat gaaccactag ccacggccac gttttttcgg
     7981 tacaatataa tattgcagag atatttccac catatctgta gttcagggaa aggagacggc
     8041 gccaggtcac gatgctgcaa ctcgataaat ctaccagcaa accctattac gtacaaatct
     8101 atgaatatta tcgccgggaa atcgaagagc accggatgat agccggcatg cgcctcgact
     8161 cggtccgtga actggcccag gctgcgggca tcagcaagat gaccgtcgaa aaggcctact
     8221 accagctggc cagtgaaggc tacatcctgc gccgccataa ggcccgctat gaagtggcct
     8281 ccctgggcgg tctggaagcg cggaccgaag aggctgctgc gcccgtattg cgtgaagcca
     8341 gtcgtcgccc tgtctatgcc tatgactttg ccagtggcga catggctttg gaacgctttc
     8401 ccctcgacat atggcggaaa tatatgaatc ggatcttatc ggagccagac cggctgctgg
     8461 cggccaatga tgaccagggc gtccctgatt tgcgccgggc cttgagccgt tacgtctatg
     8521 aaacgcgcgg cgtccatgca gagccgtcgc agattatcat cggtgccggg acgatttctc
     8581 ttctcggcgt cctgaccaat ctgcttcacg ataaatatgc ctgcatcggc gtcgaagacc
     8641 cgggcttccg cctgggccgg gagattttcc gcaacagcgg ctattccatc gtgcccattc
     8701 ccatcagcgg cggactgctc cacgtcgatg ccctggaagc cagcggcgtc cgcctggtct
     8761 acgtcagccc gtcccatcag ttcccgacgg ggacggtcat gcccgccggc atccgccatc
     8821 gtctgctgca ttgggctgaa aagacggatg gactgatcat cgaagacgat tacgacagcg
     8881 agctgcgcta ttatggccgc cccgtcccgg ctttgcaggg cctggaccgc cacggccgtg
     8941 tcgtctacat gggggccttg tccaaggtat tgcccttttt catccgcctc agctatatgg
     9001 tactgccgcc tcagctcatg cccctttacg aacagcggcg cagcctgttc cgccagggcg
     9061 cgtcggttcc ggagcagtgc gttctggcgg aatatatcga gagcggcgaa ctggcccgtc
     9121 aggtacggcg cctgcgcaag gaataccagg aaaaagggga gctcttaggc cggctcttag
     9181 tcgaagcctt tggtcaggat atcgtcgtag ggcggctggt ttccggcgta tactgccata
     9241 tccgcctgca cagccgctta tcggaagaag aactgcgccg ccgggcggaa ctgcagggct
     9301 gccgtgtctt gtccatgcag tctttttatg aaacaccgtc gcaggaaacg gataaagaat
     9361 ttctcctgtc attttcaaaa attcccacct gcaggctccg cgatgccgta gcggcgttgc
     9421 atggagcgtg gtcagagaaa gaaggatact aatggcgaaa agtttgcgtt ttattcaatg
     9481 cggggatctc cacttaggga gccctttcca taacctctcc gctattgatg aacggtggca
     9541 gcgcatcgtc gggaaagcgc cggtccgggc gttccagaaa atcgtccaga tggccattga
     9601 taaacgggtc cacgctgtcc tcatcaccgg cgatgtctac accagtgccg atcacaactt
     9661 gacggcacag ctcgattacg tgcgcctttt acataaatta gcacagaata acatccaggt
     9721 ctttgccgtc ctggggaatc acgacccgct cgaagcgtgg aaggcgaaga ttccctttcc
     9781 gccgaatgtc cacgtgtttc cgaccgatgc tgtcgaacgg gtccccctgg tcgtagatgg
     9841 cgaagaagta gcggccattt acgggcagag ttatgccaag agcgaacagc gggacaatct
     9901 ggcctggaaa ttcaaccgcg ctgctggcga ccgattttcc atcggcatgc tccataccca
     9961 ggtaggcagc cgggattcga catatgcgcc gtgcaccctg gaagatttga aagaatgtgg
    10021 catggattac tgggccctcg ggcatatcca caagcatgag atactctgcg aaaagcccta
    10081 tgtcgtctat gccggcaacc cgcagggcct cgattgtacg gaaacggggc cgcggggctg
    10141 ttattacgtc gaagtcggac cgtatgggac gacggacctg gaattcatcg acacgagcat
    10201 cgtccgctgg gaaagcatcg acctcgccat cgacggtatc gaatcggtgt cggaactgcg
    10261 cgagtccgtc cgcttcgcca aggaaaaagt gcgccgtgat atcggcaagc cgacgttcct
    10321 gacggtcaat ttcaccggtt ccggcagtat gtaccacgtc ctcaacaatc cggaggcgac
    10381 gcagtactgg ctggaaagct ggcgtgaaga agaacagggg aaatacgcct tcgtcatggt
    10441 cgaacgcctg cgtaacctgg cccgtcccaa gatgaatatg gacgaacgaa gtaaactgcc
    10501 cgactccgtc ggcgactact tgaatgtctt cgacaagctg gaaaagctgg ctcctgaaga
    10561 aagggccaag gcgctgcggg atattctcat gagccgcccg gaatttgaac gcctcggggc
    10621 ctatggaagg gccctgacgg accagcgcct gctggaaacg ttcgagaaag ccaagtggct
    10681 gggcgtacag aaattgctgg aacatagaag aggttaagca gtatgaagat tacagagatg
    10741 catttggatg atttcgggat ttaccataat gtgagctgga atccgccgga acacggcctg
    10801 atcgtcatgc atggccgcaa cgaaagcggc aagacgacgc tcatgaaata tgtccgctcc
    10861 atgttcttcg gctatctgcg cggagactgg aagggctatt tcggcagcat gggcatccgt
    10921 cgggaagacg gaaaggaata ccgcatcgtc cgcaatgaaa aagaatattt cctgtcagac
    10981 ggtcaggaaa aagtacagga agagccagcg aatctgtggt ggcacggcct ggaccgtcag
    11041 acgtatgata cgatttttgc catgggcctg gaagacctgc agggcttcaa aatcctgtcc
    11101 aatgaagccg tgcgcagcca tttcttcagc atcgaaggcg gcatcggcat gggcctggcc
    11161 cgcagtgaag tgaccaagca tatgggtgac ctcctggtcg cttcgccgca gggcaagaaa
    11221 cccatcaatg ccctgctcaa cgagcagaag gacttcgacc gccgcattac cggcatggcc
    11281 tacgatgaag atgaattcgc cagcctgcag gaaacggaac agacgacgca tgaaatcgag
    11341 aaccgcatcc gcctggatat cgaagaatcg aagcagcaga tcgaacggat ttccatgcct
    11401 atcgctgcct gggatgtcta taaacgcggc caggacgcca tgcagcatat gcaggccctg
    11461 gccgatgtat cccagttccc ggctgacggc gcgcagaaat gggccgagct ggaacagaac
    11521 atcaaggcca ttgacgaaca ggtccgcaag ctcaaggcga cgagccgcaa aggcccggcc
    11581 ttccaggaat cgtggaaccg ctggctggcc tgcggccccc agctcgacgc catggctcac
    11641 catgtcgtgg aatggaagca ggggctggaa gagctttcgg accatgaaga taaggaaatg
    11701 gactgggaat ttgaaggcac gaaacaagcc gacctgctca agtcctggac cgatggcaag
    11761 gatattccgg cagccgtcaa ctggactcag gggatttcgg ccgccgctgc cctcgaccac
    11821 agtcgtcagg atttagagaa atggcaggct gacaagccca agaatgtttc cggtgccagc
    11881 ggcgaagacg gtccggctga aaagaccaag gacgaatggg atgtcatcgg gacggccgta
    11941 gcgacgatcc agaacgtcgt catggaacgc cagaagatac aggaacagct ggaatggctg
    12001 aagaacgaac cggctggctc gtcgaaggcc tttaccttcg tcggcatcgc tttcctggtc
    12061 ctggcggctc tctgcgtcgc cgctgtctat ctggctcact tcgattcgac cctgggcctg
    12121 ggcggtgccg ctgcctgtgt cgtcatcgcc atcatttccc tggtccgcca gagtaccggt
    12181 gccgaccggg ttccccggaa aatcgcagaa atcgaaggcc agctggcagg cgtcaaccgc
    12241 aaaatcagcg acttagccaa agaagcggaa atgaccctca gtacggacga atcgaacgaa
    12301 cgctggctcc aggaactgga cgccgtccgc aagcagtatc tggactggca gacgcgggaa
    12361 acgaagaatg cctggcagaa agaacagaaa gtcatgtacg acgccattta tgaaaaatgg
    12421 cagtccgatg gcaatacctg caaaaaacag ctcctggcct gtgaaacggc ctgggacggc
    12481 tggcgcgata aaaatagttt cagcaaactg gatacggccg atgtggccaa agccaaagaa
    12541 gcctgggata cgtggcatga agtcacgacg gccgctgccg attggaagcg acgcaagttg
    12601 gaattgaaag gcgccatttc ccgctggagc gatacggcag aacagatttt ccgggaagtc
    12661 ggcgtcaaga aggccgtttc cccggatacc gtcgaagacg tctacaaaca gtggcaggac
    12721 atccgcgtcc aggctgaagt ggccaaagaa caggaccgtc agcagaaaga acgggaaagt
    12781 cagattgcca gcctgggaga agaacggcag cagcgcgtcc aagaacagca ggaactgctc
    12841 aaggcaaccg gcgcccagac ggaaggcgaa ttccgcagca aagtcctgcg cttccgccag
    12901 ttccaccagt acaaagaagt ctatgaccag acggaagccc atatccgtct catcgccaag
    12961 acgccgaaaa acctggccga actgcgccat gaattgaaaa tccatacctt gaagacctgg
    13021 acagatgaac gcgactacta ccagaaaaaa atcgccgatg ccgaaaagaa actggctgaa
    13081 gtcgcagaaa aacgaggcag tatcattgaa cgcctgagcc agatggccaa gagcgacgaa
    13141 tacggcaagc tcctgcaggc caaacagaac cgcaaggccg aactggaccg cgacgtcgac
    13201 gactggctga ccaatatgta cacccagtac atgctggaac aggcccagga atactacgaa
    13261 cgggtccgcc agcctatcgt catccgccag gccggcgaat acctcaatct catgacccag
    13321 ggccgctata ccctgcaggc tagcctggac ggcaaacagc tctttgccgt cgatggcagc
    13381 cagcgccgcg tcccggaaaa acagtggagc agcggcctgg gcgaccagat ttacctggcc
    13441 atccgcatca gcctggccat ggccttctcc aaacaaatcg aaccgatgcc gctcatcctc
    13501 gacgacatcc tcgtccgctt cgacgaacag cgccagaaag aagccatcca gttcctggcc
    13561 tcgttgggca aaaaagaaca gatattcctg ttcacctgct ccgatgccac gttgcacctg
    13621 gccgaagaag tccaaaaaca actgtctgga gaaacagaca cgatccatct ctttgaaatc
    13681 gaaaaaggga cgatacgggg aaaggaaggc ttatgctaga gtatgtagtt cgacaaacgg
    13741 cggcctacat ggaatccatg cctaaacaga ttcgcaaaaa aagtggtcaa ttctttacca
    13801 gcatagaaac agcccggttc atggccgggc tgtttgctat ccctgcacag cctgaaatta
    13861 ccatattgga tcccggagct ggcacgggta tccttacggc tgctttattg gaacgtattt
    13921 cggcaatccc agctgtggaa cgagtacacc ttatatgtta tgaaacagat gaaaaaatca
    13981 ttcctgtttt agaagataat cttcgttatg tgaccgctca ttgtttaaaa aaagctgact
    14041 atgagctccg cactttgaat tatattacaa gccaggaaaa tgctttcaat ggaggcctgt
    14101 ttccagaatc aggcatcact tgcgacc
//
LOCUS       DAWABZ010000040        13683 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_1226_length_13683_cov_3.383475, whole genome shotgun sequence.
ACCESSION   DAWABZ010000040 DAWABZ010000000
VERSION     DAWABZ010000040.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 13683)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13683)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13683
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1226_length_13683_cov_3.383475"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3062..3071
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7392..7401
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3080 a         3995 c         3692 g         2896 t
ORIGIN      
        1 tgtaaggggc ttttggacag ccctttgaga ataaggctat cctgatttca ggagggaact
       61 taaacagcag tcttctcaag aatctcccgc ttctataagc gggagaggtt caatacgatt
      121 ctcgtgctgg cagggtattt gaaaatcgta tcccaccggc gagccgcctg agccctggac
      181 ggacgacgtc gtatctgatg acgaccatgg aaaacccgcc gtcagattga tgaaaagggc
      241 tatgaaaccg atttcaagga ccggggcatt acgaaagtcc tgaaatacgg tatcgccttt
      301 tacgggaaac aggtcgtctt agtgtaaaaa ttgagtcaaa agaggtcgaa gcccaaggga
      361 aaatctttgt gcaacggcct ctttcatgat ataatgaacg ttagtctatt tatttctatt
      421 ttatggtatg tatggaagac aagggaggca gtgaagatgc gggcaatggg aatcgatccg
      481 gggacggcta tctgcggttt tggtgtcatc gacgccgacg gcagccgtct gaaaccggtg
      541 acttatggga ccatccagac gacgccggac cataccgatg ccgaacggct ggtcatgctc
      601 tttgacggcc tcaatgaact ctatcggaaa tataagcccg acgtcatcgg tgtcgagcag
      661 ctctttttta accgcaacgt cacgacggcc attaccgtcg gccaggcgcg gggcatcatc
      721 ctcctgacgg cccagcaggc gggcatccct atcctggaat tttcaccgct ccaggtcaag
      781 cagggtgtca ccggctacgg ccgggcgacg aaggaacagg tcatcgacat gaccatgcgc
      841 cttctcggca tccgccagaa aatcaagccc gacgatgcgg ccgacggcct ggccatggcc
      901 atttacgcga tttattccag tcattctatg agtctgaaaa gggaggtgca gttatgatcg
      961 gttatctgaa aggcgtgatc agccacatct tcgtcaattc ctgtttcatc gacgtccacg
     1021 gcgtcggcta ccggacctat gcaccggctt cgacgctgga cactctctgt gtcggccggg
     1081 aagccatgct ctatacttat atgagcgtcc gcgaagacgc catcgtcttg tatggctttg
     1141 ccacgcagga tgaatacgat ttgttcatgc tcctcatcgg cgtcaacggc gtcggcccca
     1201 aagtggccct gggcatcctc agcgccggca cggccgacag tttccgcctg gctgtccatc
     1261 agaaggacat caagggcctg accaagatgc ccggtatcgg caagaagacg gctgaacgca
     1321 tcgtcctgga attgcaggat aagatcggtc ccgtcgccag tgaagacgcc ggggctgcgg
     1381 atctgggcgg ttcggccgcg ccgggcctgt cgggcatcct ggcagaaacg ctggcagccc
     1441 tgaccagtct cggctacagc gaacaggaag tcctgcccgt cgccgaatcg cacctggccg
     1501 gctgtacgac agtggaacaa ctgctgaaag agacgctgaa ggccttgggg agtgggaggt
     1561 aagccatgga agaaaatcgc atcatcgaga cgacagacct gccgggcgat tcctggcagt
     1621 atagcctgcg tcctcattat ttacgcgaat atattggtca ggataaggtc aaagacaatc
     1681 tggaagtttt catcaaggcg gccaaactgc gcaaggaagc cctggaccac gtcctcctct
     1741 acggcccgcc gggcctgggc aagacgacca tggccggtat cattgccaat gaactgggcg
     1801 tcaacctgcg catcacctca gggccggcta tcgaacggcc tggcgaccta gcagcgctcc
     1861 tgaccaatct ccaggaacgg gatctcctct tcatcgatga aatccaccgc ctgtcgcaca
     1921 gtgtcgaaga agtcctctat gcggccatgg aagactatgc tctggatatc gtcatcggca
     1981 agggccccag tgcccggtcg gtgcgcatcg acctgccgcc gttcaccctg gtcggggcga
     2041 cgacccaggt cggccgcctg gcaccgcctc tgcgcgaccg cttcggcgtc atctgccgcc
     2101 tggaattcta taaaccggag gacctggtcc tcatcatcaa gcggacggca gatatcctcg
     2161 acatggccat cgatgacgac ggcgccttgg aaatcgcccg ccgctcgcgg gggacaccgc
     2221 gtattgccaa ccgcctcctg aagcgcgtcc gcgacttcgc ccaggtcgcc gaagccgcga
     2281 cgattacggc gtccctggcc aacgatgccc tgaaccggct ggaagtcgat tcgtgcggcc
     2341 tcgaccggac ggaccgccgg gtcctgcagg tcatcatcga taaattcggc ggcggtcccg
     2401 tcggcctcga caccattgcc gccgccatca gtgaatccgt cgatgccgtc gaagacgtgt
     2461 acgaaccgtt cctgatgcag cagggctttt tgaaccgcac gccccggggc cgcgtcgtca
     2521 ccgacgccgc ctaccgccat ctgggcctgc ctgcgccatc agaaggatag gagggacgat
     2581 tatggcgaaa ttactcatcc ttacgggtct ctttttcatc gtcgccggcc ttttatggat
     2641 ggccggtgat tccctgggac tggggaattt cctgggccat ctgcctggcg atatttcctt
     2701 taccaaaggt aacacgtcct tccattttcc catcgtgact tgcctcatca tcagtgcagt
     2761 ccttacgatt gtcctcaatc tcttctttcg cctgtgagga ggatttttat gtataaaaag
     2821 aagatagccc gcctggcctt atgcacggcc ctctgtgccc tggtgacgac gagtgtcttt
     2881 gcgtcgccga ccaaggccaa ggccaaaagc catccccgcc agcccgtcaa agcggtgcgg
     2941 cagacggcca aggcgccggc tggatataca cataaacaag ccgtccacga tagcgctacc
     3001 ctgcgcatcg gcatccgcga aggccggggc agtgtggccg tcaccgggcc gcagggtctg
     3061 gnnnnnnnnn ngtctgggcg tctatcgcgg cgacatgctc tggaagaaag cggcggccaa
     3121 cgtgcccgtc accattgccc tgtcggggac gaacctgacc gtcaacggcg acatcagtac
     3181 cgtaccggcc caggtccggt ccctggtcca cggcggctcc gtcaagatca ctgacggcta
     3241 cgcctaccgc ggcgccctgg aaatgatgaa atctcccggc cgctggggcc tgaccgtcgt
     3301 caacgtcctg ccggtggaac agtatctcta cggcgtcgtc ggcaaggaaa tgtcgccgtc
     3361 gtggagcgaa gaggccctga aggcccaggc cgtcgccgcc cggacctatg ccattgccca
     3421 taagagccgc ttcagccagc gcggcttcga ccttaccgac gatacgtcga gccaggtcta
     3481 tgccggcatc aatggcgaat cgccgtcgat catcaaggcc gtcaatgcga caaaagggga
     3541 gataatcact tatcagggcc ggcctatcga cgcctttttc tgctccactg ccggcggctg
     3601 gacggaaaac agtgaaaatg tctggggctc ccatatcccg tatctgcgcg gtgtcagcga
     3661 tgccagcgat gagatgccgg gttatcgctg ggctgtgacg acgacgccgg aacagatggc
     3721 ggccaaactg agtgctgccg gcaagggcgt cggccgcatc caggccatcg agttgtcgcc
     3781 cctggccaag cggcccatga cgacggccga ccgcggcgtt tccggccggg tccagtccat
     3841 ggtcgtcaag gggacgaagg gcagcgtcac cgtcaagggt tcggccttcc agtccatctt
     3901 tggcctgcgc agtacgctct tcgattttta cagcggccgc ggtacggcgc cggatcccga
     3961 ttccggagct gtcgcccgca aggccgattt caaggtcaaa gccggccagc ccgtgacgat
     4021 ttacggtttc ggctggggcc acggcctggg catgagccag tacggcgcct atcagatggc
     4081 gcgcagccat gccagtgaaa aggattatta ccgcaacatc ctgacccatt actatacggg
     4141 gacgaagatt gaaaaattat attaagctaa aaagaggaag attattttga aactgacaga
     4201 tttttactac gatttaccga aagaactcat tgcccagcat ccggccgaac cgcgggacca
     4261 cgcccgcctc atgctctatg accgtcagac cggcgccgtg gaacacaagt atttctacga
     4321 cctcgtcgac gaactgcacc caggcgacgt cctggtcttc aacgattccc gcgtcatccc
     4381 ggcccggctc cacggcaaac gggtgccgac tggcggcaag gtcgaagtcc tgctcttgac
     4441 gcctgtcggc gacgaccgct gggaagtcct ggtcaaaccg ggcaagaagg ccctgccggg
     4501 gacgaccatc gagtttcccg aaggcctgac ggggaccgtc gaagaccgga ccgatttcgg
     4561 cggccgtatc atcaagttcg cctaccaggg caatttcgac gacatcatcg acaagatcgg
     4621 tgaaatgccg ctcccgccgt atatccacga aaagatggaa gatcccaatg aataccagac
     4681 ggtctatgcc aacgaacgcg gcagcgcagc ggctccgacg gcaggcctgc actttacgga
     4741 agagctgctc cagaagatcc gcgataaagg cgtagaagaa gtcttcgtca ctctccacgt
     4801 cggcctgggg acgttccggc cggtcgaaga agaaaacatc gaagaccacc agatgcacag
     4861 tgaattctac agcatcacgc ccgaagcggc ggccaagatc aacaaggcca aggccgaagg
     4921 ccgccgggtc atcgccgtcg gcacgacgag catccgcact ctggaaagcg ccggcacgac
     4981 agggaccctg caggccggtt cgggctggac gaatatcttc atctatccgg gcttcacctt
     5041 ccacatcgtc gatgccctgg tcactaattt ccatcttccc gaatcgacgc tgctcatgct
     5101 catcagcgcc ttgtcgacgc gggaaaaaat cttgaaagcc tatgaaatcg ccgtccagga
     5161 aaaataccgt ttcttcagtt tcggcgacgc catgttcatt cactaaggga ggaaaagcta
     5221 tatggtcatt acctacgaac agcaggccga atgtgacggc gcccgcgccg ggatactgcg
     5281 cacgccgcac ggcaccttca agacgcccat gttcatgcct gtcggcacgc aggcgacggt
     5341 caagacgctg acgccggaag aactctacga tatgggcgcc caggtcattc tcagcaatac
     5401 gtaccacttg ttcctgcggc ccggccacga cctggtccag gaagcggggg gcctgcataa
     5461 attcatgaac tggaaacagg ccatcctgac cgacagcggc ggcttccagg tcttcagcct
     5521 gggccagatg cgcaagatca ccgaagaagg cgtcaccttc cggtcccacc tggacgggtc
     5581 gaagaaattc ctgtcgccgg aagtttccat ggatatccag atggccctcg attcggacat
     5641 cgccatggcc ttcgacgaat gtatccctta cccgtcggac tatgaataca cccagatgtc
     5701 gacggaacgg accagccgct gggctgcgcg ctgcctggac caccatcaca gcgatacgca
     5761 gggcctgttc ggcatcgtcc agggcggcat gtacaaggac ctgcgcaagc agagctgcca
     5821 ggacctggtc gacatggatt tcgacggcta tggcattggc ggcttgagtg ttggcgaatc
     5881 taaggatata atgtatgata tattagagca tacgacaccg tacctgccga aggataaagc
     5941 ccgctatctc atgggcgtcg gcacgccgga ctgcctgctt gaaggcgtcg cccgcggcgt
     6001 cgatatgttt gactgcgtct tcccgacgcg cgttgcccgc aacggcatgg ccatgaccca
     6061 tacgggccgc ctgaccgtgc gcaacgccaa gtatgcccac gacttccatc ccattgaaga
     6121 aggctgtcag tgttatacgt gccgcaatta ttcgcgcgcc tatatccggc atctcttcaa
     6181 ggccgaggaa ctcctggcat accgcctggt cagcatccac aacctgtact tcctgctgca
     6241 gttcatgcgg gatatgcgcc agtccatctt cgacggcacg ttccgccagt tccgccagga
     6301 tttctggaaa cgttatcaag acgcgtaagc gcttgcatat agactatttt tttatttcac
     6361 ggaggtgtaa gacatggata ttatggaaca gctcaatgct ttttggccca ttatcctcat
     6421 ggtcgtcatt ttctactttc tcttatggcg tccgcagaag aaacagcaga aaaaacggca
     6481 ggaaatgctg gaaagcttga agcccggtgc taagatcgtc accgtcggcg gtgtcatggg
     6541 gaatatcgtt tccctgcacg acgattacct cgtcgtccgc attgctgacc gcgtcgaaat
     6601 caaagtcacc cgcgctgctg tagcccaggt cctgggcaag gccgaaggca agaaagcagc
     6661 caaaaaagcg gaaaaggctg aaaaagctgc cgaaaaagcc gctgaaacca aagcagaagc
     6721 tccggctgaa gccaagagcg aagccgttgg tgaaacgaag gaagccgaag ccaaagatgg
     6781 ccagtaaaaa agaactgcgc aaagccatga atgcccgcct ccacgggctg acggcggaag
     6841 aaaaggcagc ggccagtgcc gctgtctgcg cccgtctggc cgccctgccg cagtatcggc
     6901 aggccagcag tatcatggct tttctccata tgcccgacga agtcagcctc gatgccctga
     6961 tcaaggacgc cctggctgcc ggcaaggaag tctacgtccc tgtctgcgtc gataaacagc
     7021 atatcgaagg gcggcgcctg tatacgctgg aacaggctgt ctgcggcgcc tacggcatcc
     7081 ggacggcacc gccggacaat ccgcgcatcg aagccgatga gctcgatgtc attttcgtgc
     7141 ccggcctggc ttttgacgcc aagggccacc gactgggcca tggggcgtcc tattatgacc
     7201 gcttcctggc cggaaagggc ggagcagccg cgattgctgc ggcctgggat gtccagatca
     7261 tccccgacgt gcccgtcgaa gcccatgacg tcatcatgac cgatatcgtc acggacaaac
     7321 agcatctcat tatccatcca taaaaaacta ttgaagatta cccagagggg ggagtattgt
     7381 tgcggactcg tnnnnnnnnn ncggactcgt aacatgataa aatttttcgg ggcagtcatc
     7441 atcatcctgg ccgcctttgt taccttgatt tatccgcttg ccaactccat caaacagggc
     7501 ctggacttac agggcggtac gcatatcgtc ttggaagccg aagatacgcc ggacgctccg
     7561 gtcacggaag actccttgag ccgtgccgtc accatcgtcg aacgccgtat caatgaaatg
     7621 ggcctgaccg aaccgcttgt ccagaaggaa ggcgcccgcc gtatcatcgt cgagctgccc
     7681 ggtgaaaaga atccggaaaa agccatcgag acaatcggga aaacggctgt catggagttc
     7741 aaggatgaaa gcggcacgac ccgtatgacc gggaatgatc tgaagactgc caaggaacag
     7801 attgaccagg gccataagaa tgtcgtcgcc attgaattta ccgacgaagg ggcgaagaaa
     7861 tacgccgatc tgacagcgtc caatgtaggc cataaaatcg gcatctacct cgacggcgac
     7921 ttgctcacca atcctgtcgt caacgaaccg attaccggcg gcaaagctgt catcacgggc
     7981 agccagactc tggaagacgc caagaacctg gctatcctcc tgcgttccgg tgccctgcct
     8041 gtcaaattga aagtcatgga agtccgcacc atcggcccga gcctgggcct cgactccaag
     8101 atcaagagtg aaaaggcctt tgccatcggc atcatcctga tcatgatctt cttgatcgtc
     8161 atttaccgta tgtccggcgt cgtagccaac atcgccctgc tggtctacgt cctcatcctg
     8221 ctgggcatcc tgaaatacct cgacgcgaca ctgaccctgc cgggcattgc cggtatcatc
     8281 ttgtccatgg gtttcgccgt cgatgccaac atccttatct ttgaacggtt caaggaagaa
     8341 gtcctcgtcg gcaagagcct gaggacgtct atggaagccg gtttccgccg ggccttcaat
     8401 accatcctcg atgccaacgt gtcggtcatg atcgccgcca tcgtcctcat cgtcatgggc
     8461 agcggcacgg tcaaaggctt tgccgtcaac ctggccttgg gccttgtggt cagcatgttc
     8521 acggccatcg ttgtcagccg gtccctgctc acgtggttca tcaatacagg cctcatcacc
     8581 aatccctggt tctacggcct gaaccgcaaa ccgcctgaac atatgttgaa aaagggggga
     8641 cagaaatgag ttttgatatt gtcaaacatc gcgtatggtg gttcacctta tcgtctgtcc
     8701 tcgtcattgc cagcttgatc tctatcttca tcaacggctt caacttcggc atcgactaca
     8761 cgggcggcac gatcctcgat atgcagttca ataaagccgt caccgtttct gaagtccgcc
     8821 aggtcatcga caatgccgac atggacctgg gcaacacggt catccagctg acaggcgtca
     8881 ccgaccagga ttcgtcgact gacgtcatgc tgcgcatgcg caacctgtcc agtgacgaaa
     8941 gcaaggccgt ctcggacaaa ctgagcgggg ctttaggcgg ggctgaaatc aagcggaccg
     9001 aatcggtcgg cgccgtcatc ggctcggaag tcacgaaaaa tgccgtcacc agcctggctg
     9061 tggctttcat cgccctggct gcgtacattt ccttccgctt tgaatataag atcgctattt
     9121 cggccttagt gtcgatcctg cacgacttga tcatggtcct gggcttcttc tcgttctttc
     9181 atctggaaat cgatgcgtcc ttcctggcgg ccatcctgac cgtcgtcggc tactccatga
     9241 acgaagccgt cgtcatcttc gaccgcgtcc gtgaaaacac gcggacccac cgccggacgg
     9301 actcgtatga aaaactggcc catgacagta tcgcccagag tatccatcgc agtatctata
     9361 ccctgacgac agtactcttc gcctgcggtg ccctgcactt ctttggcggc gattcgacga
     9421 agaacttctc cctggtcatg ctcatcggct tcatcagcgg ggcttattcg tccatctgcg
     9481 tcgacacgtc cctgtgggtc gtatggaaga accatacctc tcataaacgc ggcccccacg
     9541 ccgtagaagc agaaccggca gaaacagaag aataacataa aaggcttgtc gtcgtgcgac
     9601 aagcctttta aagctgttag gggttagcag ttagctgtta gcggatggtt tttattttaa
     9661 aaatttcact gttgtagggg ccgtcccaaa gggcggcccg ccacgaacca cgagccacga
     9721 acccctaacc cctagcccct aacccctatc cctaaaacaa ggaggaatca cgatgtttaa
     9781 gatcaatgct ttgggcaaag cctgcccgct cccggtcatt gaagcccaca aggctttgaa
     9841 ggaccacgat gctgtcgaaa tcactgtcga caacgaaatc gctacggaaa acctcaagaa
     9901 tttatcccag gaaatgaact gctcctatac gatgagccag gaatcggata cgcattacac
     9961 ggtccgcctg gtcaaggaag gggccgatgt cagtgaagac gaaggaccgg tcacgtcggg
    10021 ctcggactac acggtcgtca tcaatgcacc cgtcatgggc gtcggcgacg atcagctggg
    10081 caagaacctg ctcaagacct tcatctacac cctgaccgaa caggatgtcc tgccgaaacg
    10141 ggtcatcttc tacaacggcg gtgtcccctt ggtcacggaa ggttcggaat cgctggaaga
    10201 tttgaagaac ctcgaagccc agggcgtcga aatctacgcc tgcggtgcct gcctcaacta
    10261 ctatggcctg accgacaagg tcgccgtcgg ctccattacc aacatgttcc gtatcgtcga
    10321 aatgatgcgc acggccaacc gcattgtcaa accataggcc atggaagaaa cgattatcaa
    10381 agctgccatc ctcgtatcct ttatcttttg cggctatacc ttaaaacagc tgcgcctttt
    10441 cgggcgcagc acttttaata ccatttcgac gatcgtcttc aacatcaccc tgccgtcggt
    10501 catcgtcgtc aacctcaatg gcatccattt cgacacgcgc tatctgctca tcagcctcct
    10561 ggccatcatc ttcaacctgg tcatggtcgc catgggctat gccgccggcc ggacgaagga
    10621 cgaaaaagcc ttttacatgc tcaacatgaa cggctacaac atcggcaatt tcgccctgcc
    10681 tttcgtgtcc tacttcttcg acagcgccgc cgtcctcatc gtctgcatct tcgatgccgg
    10741 caactccctc atgtgcctcg gcggggccta tggcctggcc cgctgtgtgc gcggtgagaa
    10801 aggggagagc atcgtcatga tcctggccaa gacgattttt tcgtccattc cggtcttatc
    10861 gtatatcacc atgattacct tgtccctggc cggtttctcc ctgccccagg tcgtcatcga
    10921 ctgggccaag atcccggcgt cggccaatac tttcttgtcc atgctcatga tcggcgtcgc
    10981 cctgggcttg tccttgaaaa aggaatacct gcacctcatc tataccgatg tgggcctgcg
    11041 cctgctggca tccgtcgtta tagcggcctt cgtctatttt gccctcgatt atcccatggc
    11101 catcaagaaa gtcatcatgg tcctggtctt tgcacccgtt gctggcatgg cctgctacta
    11161 cacggccaag atgaagctta aaatcgaagt ggctgcctgc atcagctcat tttatatcct
    11221 cttgtccatc gtcgtcatgt cggccatgat catcgccctg cagtccgtat gagcagtcgg
    11281 ccagtaattc ttcttccagg tcggccggaa tcgtcgacgg gctgcgcgtg atgtagtagc
    11341 tgtcgacttt gggcggctcg aaatcgagga ggggaatggc ccgcaggcgc ttttcggcca
    11401 ggtcctcttc gatgaggctc ttgggcagga aggtatagcc gatgccgtcg gccacgtact
    11461 ggggcagctt ggtgctgttg tcgatttcca gggggaagcg gtgatggcgt gggaacaggt
    11521 cctggatgta gaggcccacg ccctggaggg cgaaattgca gaagaggtag tcgattttct
    11581 gcaggtcgtc cttggaaatg ccgttggcat attccgtatt gactgcctgg cagaccagga
    11641 ccagcgagtc cgtccggtag gggcggcaga tatagccgtc gcggaacatg gcgctgaagg
    11701 aaaagatcgc gtccagcgtc ccgtcctgga gctggtgcat catggccgtc gtatggccga
    11761 tggtgacgtg cagggacagg tccttcttct ctttgaggaa gctgcggatg cggcgctgga
    11821 ggtgacattc gtaaatagtg ttagtcgtgc cgatgctgat tttctgggca tacgtcggtg
    11881 acgactggat gtcctgcaga gctgccagtt ccaacgagac gaagcgctgg gcatagtcca
    11941 ggaatttctg gcccgacggc gtcaggtcga cttgcttgtg gcttcggatg aataagggca
    12001 cgcccagttc catttccagg tcgcggatgc ggttggtgac cgtcgattgt gcgacgaaga
    12061 gctgttgtgc cgttttggtg aagttcttga tttgtgccag ggcgatgaac gtccggagtg
    12121 tcgttgtgtc catggccgga cctccttcag ataatttgat ttaatttgat tatcgaatga
    12181 aataaattaa aataatctat ttcacaaatt gtctacttgt attatataat aacccatgta
    12241 gagaaacaat gaattcaatt acaagaaact ggagctgaaa taggatgaac aaaaaaactg
    12301 tcccctttaa cgaagaacaa atccgttcta tcattgccga acacccgaca ccgttccata
    12361 tctacgacga aaaagccatc gtcgataatg tccgtaaatt gattaaagcc tttgactggg
    12421 cttatgactt caaagaatat tttgccgtca aggctacgcc gaacccgtac atcatgcgcc
    12481 tcttgcagaa agaaggcgtc ggtgcagact gcagttccct ggcagaactg gaactgtgcg
    12541 acaaagtcgg catcaaaggc catgacatcg tcttctcgtc caatgacacg ccctatgctg
    12601 aattcaagcg ggccatggaa ctgggcgccc tggtcaacct cgacgatatt tccatgatcg
    12661 aattcatgga agaacgcggt cccctgccgg aatggatctg cgcccgctac aatccgggcc
    12721 cgctcctcaa aggcggcaac gacatcatcg gtacgccgga agaagccaaa tacggcatga
    12781 cccgcgaaca ggtcatcgac gccttccgca tcctcaagga taaaggcgtc aaacacttcg
    12841 gcctgcacac catggtcgtc tccaacgaac tccacgtcaa cggcctgatc aacacggcca
    12901 agatgatgtt tgaactggct gtcgaagtac agaacatgct gggtatcaat atcgaattcc
    12961 tcgacttcgg cggcggcatc ggccttcctt accgtccgga agaatcgttc atcgactatg
    13021 acgaactcag tgcgggcatc aagaaacact ttgaagaaat catggaaccg gctggcctcg
    13081 ataaatgccg catctccttt gaatgtggcc gtgccatcac cggcccttac ggctacctgg
    13141 tcacgacggc tatccatcac aagcacatct ggaaggaata catcggcgtc gatgcctgca
    13201 tggccaacct catgcgtccg ggcatgtacg gttcgtacca tcacatcacg gtcatgggca
    13261 aggaaaatgc accgaaagac cacgtctatg acgtcgccgg ttcgctctgc gaaaactgcg
    13321 ataaattcgc catcgaccgc agcctgccgg aaatcgacga cggcgacatc ctggtcatcc
    13381 acgacacagg cgcacacggc cattccatgg gcttcaacta caacggcaag ctccgttcgg
    13441 ctgaactcct gctccatgct gatggcagcg tcacccagat ccgccgggct gaaacactgg
    13501 acgacctctt cgctaccctt gatttctctg atttataatc gaatatgaat gaagggcatc
    13561 tcatgaatta aaaattttca tgggataccc tttttttatt gtaaaatttt tttgaatttt
    13621 aatctccttt taacctgatt ctgcaagaag tctcccgcct ctttaggcgg tgagatgaat
    13681 tgc
//
LOCUS       DAWABZ010000041        13416 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_1257_length_13416_cov_3.939525, whole genome shotgun sequence.
ACCESSION   DAWABZ010000041 DAWABZ010000000
VERSION     DAWABZ010000041.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 13416)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13416)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13416
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1257_length_13416_cov_3.939525"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    12098..12107
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2828 a         4156 c         3676 g         2746 t
ORIGIN      
        1 tgcccgacga atcgaagtta aaggaaattt ccacgtccgg atgttctttc tgattctggg
       61 cggcaatctg gttcatcgtt tccgtcatgc tggccgcagc aaagacggtc aggtggactt
      121 tatcgccgtc cccggcggct ttgtcctctt tcttgtccga cccgcatccg gccagggcaa
      181 aagccagcgt cgccgccaag accgatgcga ctactttttt ccacgtcatc accgtacatc
      241 tcctttttgt aaatccattt atatgcattt acttgcggcc acaatcccgg acttcgccgc
      301 gcagtaagtc gaggccatgg ggaatcgtat ccaggaagac atccatacat tcctgacagg
      361 ccttgggact gccgggcagg ttgatgatca aggtcttctt gcggatgacg ctgaccgccc
      421 gggacagcat ggcccgcggc gtaatctgca tggaagcggc ccggatggct tcggcaatgc
      481 ccggcgcctg acggtcggca acggccaggg tcgcttcggg cgtgacgtcg cgagggccaa
      541 acccggtacc gcctgtcgtc aagatgaggt cagcctggcg ctggtcggcc agccggcaga
      601 gctgttgttc caaggggccc tcgccatcgg gcaggagcag ctgttccatg acttcaaacc
      661 cagcccgttc cagccgctgg acaatgacgg ggccgctggc atcttcccgc aggcctttgc
      721 ttcccttatc cgacaaggtg ataaccgccg cctgataagg ccgtttccct tgccgcggga
      781 cttcggtcat gacatcgccc gggcggatga cgccgccttc aaggacgcgg gcaaatacgc
      841 cttccctggg catgatgcaa tcgccgacgg cttcatagac ggcacaatgg ttgtgacatt
      901 ccttgccgat ctgggtcatt tccagcagga cgtcgccaca gcgcagcagc gtccctacag
      961 gcagggccga gaaattgaag ccgtcgacga cgagattttc gccgaaagcg ccgaattcca
     1021 cctgaccgcc gcggcggcgg aaagcttcga tgctttccag gcccagcagg ctgacctggc
     1081 ggtgccagtg ttcggcatga gcgtcgcctt caatgcccca gttgactatg aaattccctt
     1141 ccggtacggc ctgtttagcc acgccgcgga tggtgctgat gcaaacagca tgtactttcc
     1201 ccatttttta tcctccgatc tgattcatcg tcttatgttc actgatatgg ttctgttcgg
     1261 cgaagcaatg cttggccggc ttgtcataaa ccgccctggc catggcgcgc tgcagctcct
     1321 gacagatagt ttcctcctct tggccgctgc ggacgatggc ccgcaaatcg acgccatcgt
     1381 catagcagag acagggcttc aaaaagcccg tactcgtcag gcgcaggcgg ttacaggacg
     1441 agcagaagcc atgggtattg gcagcgatga agccaatacg accttgcagc tggccgctcc
     1501 ggtaatacga agccggtccg tttccccgcc gttcttctgt ggcagccaaa tccggatagg
     1561 cccggcgcag gacggccagg gccgcttctt ccgtaaggcc tttcagcgtc gtcccgtagc
     1621 cgatgggcat gagttcgatg aagcgcacat cgacgggaaa ccggccggcc aagcgggcca
     1681 gcggcaggat ctggtcctgc gtttcttcca gtaatacggc gtttactttg acgtttaccc
     1741 gagcagccgc ggcccgttcc aaggcttcca ggacgacgga cagctggccg cctccggtca
     1801 gcccggcata ctgcccggca tcgaggctgt ccaggctgat gttgatgccg ttgatgccca
     1861 tacggaccaa gtccggcagg gcgtcggaca acagcaggcc gttcgtcgtc aaggtcacag
     1921 aacggacgcc tttcatggct ttgagggatt ccatgaaggc cacggcccct ttgcgcacca
     1981 gcggctcgcc gccggtaatc ttgaagcggt cgatgcccag ggacagagca gcccgacaga
     2041 tgagctgcat ttcttcatag cgcaggacag cttcgtggcc gacgctggtc agggcttcag
     2101 gcatacagta gcggcagcgc aggttgcagc ggtccgtcag ggacaggcgc aggtagccga
     2161 tggtccgtcc ccatttatcc ttcatggtgc tcccctccgt catattggaa atggccgctc
     2221 ttgccgccga ttttttcgac gaggtggata tccttgagac acatgcgctt atcgacggcc
     2281 ttgcacatat cgtagaccgt cagcaaagcc acggatacgc ccgtcagggc ttccatttcg
     2341 acgccggtct tgccgttgca cttgacagta cagcggcctt cgagttcaca agtatcgtcg
     2401 tggaagatga attcgacgcc gcatttggtc aaattgagga tatggcagag cgggatgagg
     2461 tcgctcgtcc gcttggctcc catgatgccg gcaatgcggg ccacgccgag gacatcgcct
     2521 tttttcacgg caccggccct gacggcttca tagcactcgc ggctcatgga aatacggccg
     2581 caggccacag ccgtccgggc cgtcacggct ttaccgctga cgtcgaccat gacggcgtca
     2641 ccgttgttgt caatgtgtgt aaacgggttc accaggatca cctactttcc aaagccacag
     2701 ttcgggaagg tacagacagg acagcccagg cataagccgc cttcgccgag accggccagg
     2761 tcatcggccg tcacggggac atcggccatg agcgacggca ggacgaggtc aaagatcgtc
     2821 cgcttggcat acatgacaca gcccggcaga ccgacgatgg ggcgatcgtc cccgctataa
     2881 gccagaagga acatggcccc tggcaggacc ggagcaccat acgatacgat gcgggctccc
     2941 gtattcttga tggccagcgg cgtctggtca tcggggtcga cactcatgcc acccgtacag
     3001 aaaaccatat ccatgccctt atccagcata tcctggatag ccgccgtaat ggcagccggg
     3061 tcatcggcca gggtcgtatg catggccatg acggcgccgt attcagacag cttgctttcg
     3121 atgaccggcg taaacgtatc ttcgatacgg ccatggaaga cttcattccc cgtcgtgacg
     3181 acgccggctt tcttctggcc gaagggatgg acgtcgagga gcggtgccgg accggctgct
     3241 tcctgggcag cggccatctt atccttcttg atgaccaagg gaatgatgcg cgtaccggcc
     3301 agtttatcgc ccttgcggac cgggaagccg ccgtggcggg tggcaatcat catttcgccc
     3361 agggaattga cggctttcaa gccatcccgg cggatagtca ggacgccgtc gcagtcggcg
     3421 atgagttcga ttttcccttc tttcggcgca ctgccgtgca tatgagctcc catgcagatg
     3481 tcgcgcagga tggcagcggc ttcatcttca tggaacaggg tgtcatcttt ttcccaaata
     3541 tagacgtggt ccttgccgac cgacaagagg accggaatat cttcttgggt aatgacatgg
     3601 cccttgcgga agaccgggcc tttggtgacg ccgcggatga tctgcgtaat atcatggcag
     3661 aggacatggc cgacagcgtc ttcggttttc atcattttca tacttatcta tccttccatt
     3721 ttttgtaatt tatggagttc ttcatcattg aaccattgcc ggaactggcg gtctgccacg
     3781 tcgtgcaggg cctcgttcat ctcttcatac cgttccagga tgatttcagc ctgcttggtc
     3841 aggacggacc ggccgccgtc tttgccgccg acatagcggc ggatgagggg aaagccccag
     3901 atttcttcgg cgttgcgcat gactgtccag gccttggagt acgacatgcc catatcccgg
     3961 gctgcgccct gcagggaacc atgacggcgg acaccttcca gcagttcggc cacgccgggc
     4021 ccgaaggccc gcttatcgcc catgaggcac agggtcactt tgcaatgtag tttctcttct
     4081 gccatggagc cacctccttc aagcgttatt cgcgcttaca aataacgaat ctactaagct
     4141 cattatacat gaaaatctgt tagctggcca tacttttttt gtaacccgta gtgaaatcat
     4201 caccgcttat gcaaattatg atataataca cgcataacat acaaaaggat gtgataggat
     4261 gacaaaaata cttcgccctt tatcgctggg gctgattatc ctggccggcg gccgcagtac
     4321 ccgcatgggc cgcgataaaa cggccctccc ctggcgcgat acggacctgc tgacggatct
     4381 gcttctgcgc agccaagctt atccctttac agagcgcttg ctttccatca accgtcctta
     4441 tgaatcgtcc catttacccg ccaaattagc ccgcacagtc cgcctggtca tagacaatta
     4501 tcctgactgc ggccccttgg gcgggatgga ggccgccctg tacagcggcc agtgcgactt
     4561 ctatctggtc ttatctgtcg accttccctt ctatgatttt gcccccgccc cggatttggt
     4621 caaattactc cggcgccagc ccaccctgca ggccatcata cccaggacgg aaaacggcca
     4681 ggaccagccc ctggccgcct tatacagccg tcagctcctg ccagccatta cggaccatct
     4741 gcagaagggc aattaccgcc tgcgccatct gtatcaagac gtgccggcag cctatgtcga
     4801 tgaaaccttc cgttcggcct tatatttcaa tatcaatacg cctgaagcct atgaggctgc
     4861 caaaggccgc gacaccaaca gccgccgcca ggtacccgtc gtcaccctga cggcaccgaa
     4921 atcaggcgat ggcaagacga cagccgctgc ggcagccatt acggaactga cgcaccgcgg
     4981 ctatcaggtc gcctatatca agagcagcca tcacaccgtg gcccgccgca agaccggctc
     5041 cgatacggac cgggcccgca aagccggtgc cgtttccgtc tgtctctgcg gtccggcaga
     5101 ttatcccatg ggaacgcgga aagaagatta catcctggcc ctgtcccagc aacagctggc
     5161 cgacctggtc ctcgtcgaaa gccgcagtca cggtcccttt ccacaaatcg acgtgaccca
     5221 ggccgatgac aaccagctgg tcaacaatat cttattcctc actggctacc gctcgtcggt
     5281 gatataagca gtttgttctt ttctgtcttg caccctgcta aaataagtcc tacactgaga
     5341 ctagtaaagc gtaccccgtc cgtggtatac taaaaatctc cagtttatgt aaaagtgagg
     5401 tgttcctatg tgtgcctata agaaaatcga cacggtctgt ccctattgtg gcacaggctg
     5461 cgtcatcacc tgcacggtcg acacggaaaa aaaccaggtc atcgaagcca aggtctcgcc
     5521 gcgcggtgtc aacaatgaag gccggctctg tctgaaaggg ctgtacggct gggactatct
     5581 gaatgaccct cagatcctga ccaagcgtat ccgccagccg atgatccgca aaaacggcaa
     5641 gggcacgccc ctcgtcgaag tatcgtggca ggaagccatc gaatacgcag cggcccgcct
     5701 caaagccatc atcaaggaat acggcccgga tgccgtcatg ggcgccggtt cggcccgcgg
     5761 tcccggcaat gaggccgctt acattacgca aaaattcatg cgcgccgtcg tcggcacgaa
     5821 caacgtcgac cactgcgccc gcatttgaca cgctccatcg gttaccggac tcggtgagtc
     5881 cgtcggtgaa ggtgccatgt ctttcagcgt tcccgaactc gaagacgcag aagtcatctt
     5941 cgatatcggt tataacgcgc cggtctcgca tcccctcgtc gcccgccata tcgtccgggc
     6001 caagaagaag ggctgcaaga tcatctgtgt cgatccgcgc ctgacagaaa cgggccgcat
     6061 cgccaccatt tacctgccta tcaagggcgg cacgaacatg ctcttcctca acgccatggc
     6121 ccacgtcatg gtcacggagg acctggtcga ccacgatttc atcgaaaagc atacgacagg
     6181 ctttgccgac tatgccaaag aagtggccca ggataaatac gcgcccgaag ccgtcgccgc
     6241 agcgacccac ctcgatcccg acgatatccg ccgggcagcg cgcctgttcg cttcgtccaa
     6301 acataccatg accctctggg gcatgggcat cacccagttc tcacagggcg tcgaatgcgt
     6361 catcgcctgc tccaacctga gtctcatgac cggcaactac ggccattacg ccaccggcgt
     6421 cggcccggtc cgcggccaga acaacgtcca ggggacctgt gacatgggcg tcctgcccaa
     6481 ccagtatcct ggctatcagg acgtcatgga cgatgaaatc cgccataaat ttgaaaaggc
     6541 ctggggcgtt tccctgccat cccacgtcgg cctgcccctg acctatgtgc cggacaaagt
     6601 cctgcgcgaa ccggacccga agaagcggat tcacgcctat tacatcatcg gcgaagaccc
     6661 ggcccagtcc gatccggacc tgccggaaat ccgccgcacc ctgtcggcta tcgacctggt
     6721 catcgtccag gacatcttct tcaacaagac ctgcgaatat gccgatgtca tcttcccggc
     6781 tacggcctgg ggcgaacaca atgccgtata tacctcctgc gaccgccgct tccagcgcgt
     6841 ccgcaagctc atcgaacccc agccgggctg caagacggac tgggaaatcc tctgcctcat
     6901 ggcaacggcc atgggctatc ccatgcatta cgacaatacc gaacagattt gggacgaaat
     6961 gcgctccctg tgcccgaaat tcgccggtgc cagctatgaa aagatggtcg agaaccacgg
     7021 cgtccaatgg ccctgcacgg aagacagccc ggatgatacg gggacacagt atctccacaa
     7081 gggcgctcat tttgctacgg ccgacggcaa aggccatttc tactactacc cctggacgcc
     7141 ggccaaagag ctggaaagtc ccgaataccc cctgtccctg tcgacagtcc gcgaagtcgg
     7201 ccactattcg gtccgtacca tgaccggcaa ctgccgcctc ctggccgacc tggccgatga
     7261 accgggctat atccagatga atcccgaaga ctgccaggaa ctgggcattg ccaacggcga
     7321 cctggtccgc gtcgtctcca agcggggcca taccatcacc cgctgccagg aaacagaccg
     7381 cgtatccaaa ggggcgacgt acatgaccta tcagtggtgg gtcggcgcct gcaacgaatt
     7441 gacgtcggcc gccctggacc ccaagagcca tacgccggaa ttcaaatact gtgcctgccg
     7501 cgtcgaaaaa atcgacgacc agaaacaggc cgaacgcgat gtaaaagccc agtataccgc
     7561 tatccgcagc cgcatgggca tcgaagtaaa ggaggtgtaa acgatgttta aacacgtcaa
     7621 gacggacccg gaactgtgca tcggctgcaa gacctgcatg gccgcctgtg tggccagcca
     7681 tacaggcaag gccctgtttg ccctcaagcc caagagcttc gatttcacgc cgcgggtcca
     7741 cgtcgtttac acgtcgaaag tcaccaaggc cgtccaatgc cagcagtgcc cgaagccgcg
     7801 ctgtatggag gcctgtccgt tccactgcat ttccctgggt cccgactcgg tcgtcatcga
     7861 tgaagaggcc tgccggggct gcggaaaatg ttcccgcgcc tgtcctttcc aggccatcaa
     7921 catgaccaag atctaccacg gcgataccaa aggccggccc ttccgcatcg tcgcctacaa
     7981 atgcgacctc tgtgccaata cggaaacggg ggaaccagcc tgtatcccgg cctgcccaac
     8041 ggaagccctg agtctcttcg acccggccgt cgtcgcggct cagcgggcta aactgaaagc
     8101 ccaaaaagaa gcggccaaaa cacaggcagc ttatggaaaa gcattgaaaa aaggaaagcc
     8161 ataactatgg aaatcgtcac gattaccgaa gcaaggcgcc gtctcgacgg cgccttgcct
     8221 tcctatgaag cgaaaataga atatgtcaca ccaaaccagg ccctgggccg catcacagcc
     8281 cgggacatta cggcagcctg cgactatccg ccgtaccgta aatcgccctt cgacggctat
     8341 gctatccata gccatgacgg aaatgcctat gaagtcgtgg cgaccatcgg cgccggcatc
     8401 gtctatgacg gccctgttgc agccggccag gccgtacgcc tcatgacggg ctgcgccgtc
     8461 ccggatgatt gcgataccgt cgtcccccag gaatacgtcg aacgccaggg aaatcatata
     8521 cgagttacag aaagcatccc gccgggcagc aacatcatcc ccaaaggcga agagtgccgt
     8581 tccggcgccg tcatcatccc ggccgggacg aagctcacag ccggtgccct gtccgtcgcc
     8641 gtcggcctgg gaaatgaacg ccttcccgtt tacgccaaag cgaaggtcct cttcctgacg
     8701 tcaggacggg aactggtcct gcccggagaa gtgcggcacc atggccagat ttacaactcc
     8761 aacgcctatc tcttccagcc cttattggaa cagtacggcg cagaagtcac cttctatcac
     8821 gtatcggacg gaccggacca gctggctgat gaaatggcag ccgtccgccg cctggcccgg
     8881 gacaaggacc tcatcgtcag cacgggcggc gtctccgtcg gcctctatga cacgatgccc
     8941 cagatttatc aatccctcgg tgccgtccaa ctttacgacc gcatcactat gcggcccggt
     9001 tcggcttcct atggcggcgt cattgaaaca gacggccgcc ggacgctcat cctcggcctg
     9061 tccggcaatc cttcggcgtc gttcaatgcc ttccatctcc tggccgtccc catcctgcgc
     9121 cgcctcaacg gcgaaagaaa cagcgaattt cccgtcgtca cctgccattt aggcgacgcg
     9181 atctataaga aaaatcctgt cgaccgcttc gtccagggcc gcctgaccta cgaacagggc
     9241 cagcccatat tcacacccaa tacgacactg acgtccagtg ccctgctggg ccttgcccac
     9301 accaacgccc tggcgtatat aaaaaaagga acggccccgt tgccaaaggg cgctcccatt
     9361 gaagtaatat tcctataaaa agggcttgtc gcacagcgac aagcccttgt ttgcactgta
     9421 ggggtcgcca cgtgggcgac ccgttagaaa ttgggacgat tttttagcaa ccacttcggg
     9481 aaatcataca tatcccgaag cgggctcgcc acgtgcgagc cctacataaa aaagctgtca
     9541 cacgaagcca aaatgccttc atgtgacact ttttttatat tataataaaa tccgttctgg
     9601 atgagcaaat attttgaaag aatcaggccg caagccgcag gacagggtaa tctggtactg
     9661 ctccgccagg gacacgccca gactcgtcgg caaggcccgc gatgccagga agcgaacgcc
     9721 caggccatgg atctttttga tgaccgattg gggaacgcgg ccgctgaaga ccaggacggc
     9781 ctgactgacg tcgatgtgct gtaattcgac gaagccccgc aggcggtcga ggacattgtg
     9841 gcggccgata tcttccgtat agacgaggac ttcatcgcct tggaccaagg ccccttcgtg
     9901 gacgccgtgc gaggcgtgat gtgccgtcga taagctgtcc aagagacctc cataggaata
     9961 gagcttctct gccgtaatcg gcacaaaagg cggcttttct actgcccgtg ccgtcccggt
    10021 ttccgtcgtg acccaggccg ccccatcgtg caggcagaca ccagccagct tgcggccagc
    10081 cggtattttc tcttcggcca gacagtaacc cacagccagg acatcgatat cttccggcga
    10141 agaaatgagc gtactgatgc gctgcccatc gagatagagc ggcgtcatca cttcgacggc
    10201 aatggactgc ttcttagctg ctgcgccttg gtaatagtcg atatagggca gctcccggca
    10261 cgacgaaaaa tgtaagtcat tctgattcat cttcacgaaa attccccctt ccccatccag
    10321 agcagggccg tcaggacggc gccgccgagg ctgcgggcct tgtcggaaat cgtaaagcaa
    10381 ttctcccgtt catcatcccg cgggtcgatg tcggctatct tgaagccttg cggtacctga
    10441 tagccgtcac ggatcagtcc ccggacgaat cccgtcagcg acgccagaat cggcgtcccc
    10501 atacattccg tcacagccat tccaaaaggg atgggctctt ctgtcaggac agccatgggc
    10561 tgtccttttt ttactttatc gccgatgtgg accaatccgt atacgaaccc ggcctgctga
    10621 ctgtggatga cgcgctcttt gccgtagccg gcaatcaatc cgggaatccc cgtattggcg
    10681 atagccgtgc cctgccaaat gatacgcccc agctggtggc cccgcttggt ttcaatgacg
    10741 gcatcgacat cgaccccggc cgtaaatccc ggccccaggg ccacggtatg acgtgccata
    10801 cgccggttcg tccccaagtt cttcttagcc aggatggcat cgacgacggc ccagggcttc
    10861 agcgccgcaa tcgacgcccc ttccgggtcc accagcaagg gaatggcctc gccttcccag
    10921 gacttccgga cctcggccaa agaccgacag cggcagcaag tcagattctc gacggcagcc
    10981 gtcccatcgt agacggcttc acagagggcc acctgacgcc ggatagccga cggcttctcc
    11041 acttccagca gcagcaggcg gaacccagcc tcatacaaag actgggccac gcccgtcgcc
    11101 aggtccccag ccccgcggat aatgaccaat ttatcttcca taagaccacc tttccacatc
    11161 gaaacatcaa acatccctat tataagagtg cttgtcgcaa gacgtcaagc actctttagt
    11221 tgttagtggc tagcagacgg ctttaaattt aaggccctct tctacaaact acaaactctg
    11281 tactacaaac ttttatttct ttttcttccg gctatacggc gtcccttcta agggcaatgt
    11341 tgtctggaac tggccgttcc atttatagta ggctccggct acggcaggtg ccgtcgggat
    11401 ggaggtgatt tcgccgacgc cgatggctcc gtaggctacg tcgacgcctt ctttttcgat
    11461 gggaatggct tcgacgtcag gggctttatc ggcccggaac aggcccaggg tcccgaattt
    11521 ggccgtcggt ttacagtggt ccaacggata ttgttccgtc agagcataac cgcagcccat
    11581 gacggtgccg ccttcgatct ggccttccag gctcttgcgg ttgacgacgc ggccgacgtc
    11641 gtgggcggcg accattttgc ggatggtacc gtcgtcattg aggatgcaga cgtgtgtggc
    11701 atagccgtag gcgacgtgtg atacgggatg agggacgtca gcgcccatct tgtcggtctt
    11761 gcccaggtat tcgccgatgt attcccgacc gttgaggaca tctaagtcgc tacggttgaa
    11821 gcggacggcg gccgtagcgg gcgattcttt ttctaccgtt acgccttcca ggtagttgct
    11881 ggcatggctc gtttcttcat aaggcaggcc cttggcggca aagaggtcgc gccgcagctg
    11941 gacggcggct cgccgggccg cttccccggt caacgtcgtc tgccgcgaac ccgacgtcgt
    12001 accggcatcg ggtgaggtcg ccgtattggg catatgccat ttgatatctt caatgggaag
    12061 acctgcagcc tgggatacca tctggaccag gaccgtgnnn nnnnnnnctg tcgtctttga
    12121 cgaccagacg gcagcggcca tagtcgggca ggccgacgcc gacgccggaa ttcttcaagg
    12181 cacaggcaat gccaacataa ggcccgctgt gatagtacgc ctctttgacg gcatccagcg
    12241 tttcggccaa gcctgtcgac gggtcggcaa tctggccatt gggcaggatg tcaccgggcc
    12301 ggatggcatt gcgatatcgg atttcccacg ggtcgatgcc gaccagttca gccagtttat
    12361 ttaagtttaa ttccgtggca aaaatggtct gagtgacgcc aaatccgcgg aaggctccgg
    12421 ctggtggatt gttagtgtaa acggctgtcc cgtcgatgtc gatggtctgg tagttataag
    12481 gccctgccgc atgggtacag gcgcgctgga gaacgggtcc gcccagggag gcataggccc
    12541 cgttgtcggt gacggcgacg agcttcatag ccgtcagttt cccagtttca tcgcaggccg
    12601 tcgtcatgtc catgctgaac tgatggcgct tgggatggat gaggatgctt tcctggcgcg
    12661 tcaacttcac tttacatatc cgtttacata cataggcgac gagggcagcc aaatgctgga
    12721 ccgtcacgtc ttcccggccg ccgaagccgc cgccgacgaa gcagttctcg acgatgactt
    12781 tttcttccgg caggcccagc atgagtgccg tttcccggcg cgtatcataa atgccctggt
    12841 ccgacgaata aacgaagaca ccgtcatcaa agggcatggc gacggcacat tccggttcca
    12901 ggaaggcgtg ttccgtccat ggcgtttcaa agtggtgcgt caccttatac ttcgaggcgg
    12961 caatgacggc atcagcgtcg ccgcggacca ggtgttcatg ggccaggacg ttcgtaccag
    13021 ccgacgggtg taccagcggc gccccttctt tcagggcgtc ggctgcacag gtcagggccg
    13081 gcagttcttc atattcgata tcgacgagct tcttagcctg ttccaggatt tccggcgttt
    13141 ccgcagccac cagggcgatg gcgtcgccca ggaagtgcgt cgtcgagccg acggggatga
    13201 gcgtatccca atcggtcttc aaatggccga ctttgacgga cccggggata tcgtctgccg
    13261 tcaggacgca gacgacaccg ggcagggcct tggccttttc cgcatggatg gccttgacga
    13321 cagcccgcgg ataagcgctg cggatggcac tgccgtaaca caggccgggc aggtcgacgg
    13381 cgtccaaatc atctgtatat ctgccgacgc ccagga
//
LOCUS       DAWABZ010000042        13317 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_1273_length_13317_cov_2.828382, whole genome shotgun sequence.
ACCESSION   DAWABZ010000042 DAWABZ010000000
VERSION     DAWABZ010000042.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 13317)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13317)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13317
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1273_length_13317_cov_2.828382"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1594..1661
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3090 a         3908 c         3733 g         2518 t
ORIGIN      
        1 cccgctgggt atatgtgaga tacttgtgac cgatgggtgt gagcgggcgc ccaacgtggc
       61 gcccctacag aaaaaagagc tgtcgcatgt gcggcagctc tttttttaca actaatttga
      121 ctttttgact tatattctct tgacaaaaca tattctccat gctatcttat atatagtctt
      181 agaagtcaaa tagtcagacc actaaaaatg gtcaaatgag cagaaggtga gtacgatgga
      241 tttggatgca cgaaaacaga aaatactgca agccattatt caggattaca tctcctctgc
      301 cgaacctgtc gggtcgagaa cgattgcccg caagtatgac ttaggcgtca gcgccgcgac
      361 gatccgcaac gaaatgttcg atctggaaat aatgggctac ctggaacagc ctcacacgtc
      421 gtccggccgc atcccgtcgc tgaagggata ccgcttctac gtcgactgcc tgctcaagcc
      481 gacgaagatc acggacgcag aaaagcaatt cgtccgcgcc tggttccagg acaaagtgag
      541 cagcgtcgac cagatttttc agtcgacggc caagttactg gccaggatca ctcacaacgt
      601 caccctggtc atggcgtcac agcggctcaa ttccaagctg aagtacatcc gcttcttacc
      661 gctcgatgac cgccgggcca tcatgatcgt cgtcaccgat acgggacagg tagagaactg
      721 catttacccc aaaccggccg gtgccagcct ggacgatctg aacatcatcg cccagcggct
      781 gaccaactac ctggccggca tggccatgga ccatatcgat gaaaaggcca tcgaagcctt
      841 tcacgagacc atcgtcgacg acgtcgaatt gtaccgcctg gccttccggg ccatgggacg
      901 ggccttccac aaagaccagc agttctacaa aggcgggacc atggagctcc tgaacaagcc
      961 ggagttcaaa gacgtcgaca aggccaagag cctctttacc atgctggaag aacaggatgt
     1021 cgtcgccaat ctccttcacg acgagggcga aggcggggcc gtgacggtcc gcatcggcga
     1081 agaagcgaaa ttgtcgtcta tcaatgattg cagcatcatt gaagcgacct ttaccgacca
     1141 tgatgtagtc ctcggcaagc tggccgtctt gggaccggct cgtatggagt atgccaagat
     1201 catcggtctt ttggacttta tgaaacagca tgtgacacat atgctggcac attaccacga
     1261 cgataagtag ggaggggtac acagacatga gtaagaaaga aaataaagaa gaaaaagata
     1321 ccgtattagc agaagacgcg gaaaaagtcg ccgaagatgc tgctaaggaa aaggtagacg
     1381 aagccaaggg aaccgcggca gatgaaggcg ccgataaaaa ggcaccggat gccaaagacg
     1441 ctgctgccaa agcggaagcc gacaaggccg ccaagctcca gcagcagttc gtccggctcc
     1501 aggcagattt tgctaactat aagaaacgca cagcggctga aaagctccag atttcagaag
     1561 tcgtcaagat ggacgtcatc actaacatcc ttcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggataaggt ggccgatgat
     1681 gtgaaatcct tcatcgacgg ctatgaaatg atttacaaac agctcctggg cgtcctggaa
     1741 aaggaagggg tcactaagat cgatgctgtc ggcaaacctt tcgaccccaa ttatcatcag
     1801 gccgtcatgc gcgtgcccag cgacgaatac gatgacgacg tcgtcgtaga agtcctccag
     1861 gaaggatatc tcctgggcga caagacgctg cgtccggcca tggtcaaagt tgcattcaac
     1921 ggttaatcat cgtttgaaat aagattggag gaatctttta tgagtaaagt tattggtatt
     1981 gacttaggta caacaaactc cgtagtagct gtcatggaag gcggcgaacc tacggtcatc
     2041 acgaatacgg aaggatcccg cctcacaccg tctgtcgtcg gcttctccaa gacgggcgaa
     2101 cgcctcgtcg gccagctggc aaaacgtcag gccgtatcca acccggaaaa caccatttcg
     2161 tccattaaac gccacatggg tgaaaactac accgtagaca tccagggcaa gaaatataca
     2221 ccgcaggaaa tttccgctat gatccttcag aaattgaagg aagacgctga aagctacctc
     2281 ggcgaaaaag tcacccaggc cgtcatcacc gttccggcat acttcaacga cggccagcgc
     2341 caggctacca aagatgctgg taagatcgcc ggcctcgacg ttctccgtat cgtcaacgaa
     2401 ccgacggcag cagccctggc ttatggcctc gataaaggcg gcgacggcaa gatcctcgta
     2461 ttcgacctcg gcggcggtac tttcgatgtg tccatcctcg aactcggcga cggcgtcttc
     2521 gaagtaaagg ctaccaacgg caacacgcac ctcggcggcg atgacttcga taacaaagtc
     2581 atggaatgga tgatcagcga attcaagaaa gaaacgggca tcgacctctc gcaggataag
     2641 atggctgaac agcgcctcaa ggaagctgct gaaaaagcta agatcgaatt gtccacggtc
     2701 ctctcgacca atatcaacct gcccttcatc acggctgatg ctacgggccc gaaacacctc
     2761 gatttgacct tgacccgtgc taaattcaac gaattgacgg aagacctcgt ccaggctacg
     2821 atggaaccga ccaagaaagc catcgctgac tcgggctaca ccatcgacga aatcgacaag
     2881 atcatcctcg tcggcggttc cagccgtatc ccggctgtcc aggatgccat caaatccatc
     2941 ctcggcaaag aaccgagcaa aggcgtcaac cccgatgaat gtgtcgctat cggcgcagct
     3001 atccaggccg gtgtcctcgt cggcgatgtc aaagacgtcc tgctcctcga tgttacgccg
     3061 ctttctctgg gcattgaaac cctcggcggt gtcttcacca agatcatcga ccgcaacacg
     3121 acgattccga cttccggcag ccaggtcttc tcgacagctg tcgataacca gccgtctgtc
     3181 gacgtccacg tccttcaggg cgaacgtgaa atggcagccg acaacaagac cctcggccgc
     3241 tttgaactga ccggcatccc ggctgctccc cgtggagtac ctcgtatcga agtcaccttc
     3301 aacatcgacg ccaacggcat cgtaaacgtt tcggcaaaag acctcggcac aggcaaggaa
     3361 cagaagatca ccatcaaatc ttcgtcgggc ctcgacaagg acgaaatcga ccgcatggtc
     3421 aaagaagctg ccgctcacga agcggaagac aagaaacgta aagaaggcat cgaagctaag
     3481 aacaacgctg actccctcat ttaccaggcc gaaaagacca tcaaggatat gggcgacaaa
     3541 gccgacgctt ccaaagtttc ggaaatcaag gataagatcg ctgccttgaa agaagccatg
     3601 aagaccgacg atacggataa gatcaaagcc gcttccgaag ccttgaccaa accgctctat
     3661 gacctgacca gcgaaatgta caagaacgca gacgctgccc agcaggctca gcagcaggca
     3721 gccggtgccc aggccggtgc acagcaggcc cagagcgatg gccagaaaga tgaaaaagta
     3781 gtcgacgccg attacacggt agtcgacgat gacaagaagt aagatagata agaggggctg
     3841 cccacatgag taaaagagat tattatgaag tcctcggcgt tcccaaagac gccagcgacg
     3901 cagaaatcaa gaaagctttt aagaaattag cgctcaaata ccatccggac cgcaacccgg
     3961 acaacccgaa ggcagcagaa gaaaaattca aggaaatcaa cgaagcgtac agcgtcctgt
     4021 cgaataaaca gaaacgcgcc cagtacgacc agttcggtcc ggcagcattc cagaacggca
     4081 atgctgccgg ttccggtgcc ggtttcggtg ccggtttcgg ccagggcggc ttcggaggtt
     4141 ttggcggctt tggcggattc ggccagggcg gccagggctt cggcggcttt gaagatatct
     4201 ttgattcctt cttcggcggc cagggcagcc gtcagtcggc caatggcccg cagcgcggtg
     4261 ccgatttgcg cttcgacgta gacattacct ttaaacaggc tgcctttggt acggaaatgg
     4321 aaatccaggt cccgaaagaa gaaacgtgcg atcactgcca cggcaacggt gccgaaccgg
     4381 gaagcaaggt agatacctgc ccggactgcc acggcacagg ccagcagcgc gtcgtccaga
     4441 acacgccttt cggccagatg gtcaatgtcc gcacctgttc ccgctgcggc ggcacaggca
     4501 agatcatcca tgacaagtgc aagaaatgcc atggcacggg gacggtcaag gtccgcaaga
     4561 aactgaagat caagatccct gccggtgtcg acgacggcgc acgcctgcgc gtagccggtg
     4621 aaggcgaacc gggcgtccgc ggcggcggtc acggcgactt gtacgtctat atcttcgtcc
     4681 gccgcgatcc caagttcacc cgtgaaggca acgacatctt gtcccaggaa acgatttcct
     4741 tcgcccaggc cgccctgggg tcgaagatcc tcgtcgaaac actggacggc aaaatcgagc
     4801 tgaccatccc ggccggcacc cagacgggga cggtcttccg catccgcggc aaaggcgtac
     4861 cggtcctggg gtccaagaac cgccgtggcg accaccacgt ccaggtcacg gtctccgtac
     4921 cgacgcacct caacgccaaa cagaaggaac tcctgcggga attcgcctat gctggcggcg
     4981 aaacgtgggt gggggagcca aagccggctg aacaaccggc cgacggtgac gccacgagcg
     5041 cgaagagcga caagaaagat acgaagaaga ccaagaagag tttttggagt aagttaaaag
     5101 aagatctgta aagataaagg gagtatgaac gatgcaatgg aatgaagtag atttgctcgt
     5161 ttccccaacg gccaccgatt tggtggccct tcttgttttt gaatgtggaa gcaatggctc
     5221 ggtcatcgac gacgagcacc cggaccacaa aggacgcatc cgcattacgg cgtatttccc
     5281 gctggcacag gaaggtcttg tggaaacgat acgagaaaag atgggcgccc tggaaaaacg
     5341 gggcgtcgac ctgggcgact ggaaggtcgt cgaaaagaaa gctgacgata aggactggct
     5401 ctttgcctgg caggaacact tccatcccaa gaagattgcc cgccatttct gggcggcccc
     5461 ggcgtgggaa ccggcccgtc cggccgacgg agaagacgtc ctttccatcg atccgggcct
     5521 ggcctttggc agcggcttcc acgacacgac gtgcatgtgc gtccagtacc tggaagatac
     5581 ggtgaaggcc ggcgatacgg tctttgacat cggcacgggc acgggcatcc tggccattgc
     5641 cgccgccaaa ctgggcgcat cccacgtgac ggctgtcgac ttcgacggcg tcgccgtcaa
     5701 gcaggctgcg gtcaatgcca aactgaacgg cgtcgaagac aaattgacca tcgccaacag
     5761 cgacctcctg gcggccatcc cgcaggggga aaaggccgac gtcgtcgtcg ccaacttggt
     5821 gaccaatgcc gtcctggccc tgctgccgac gctgccgccg tatctcaagg aaggcggtac
     5881 gctcattgct agcggcatca tcgacgaacg catcgacgaa gtgcgccgcg ctgccgctgc
     5941 cgtcggcttc acctgggtcg atgaacagtt ccgcagcggc tggtatgccg tccagatgaa
     6001 gaggtgagac tatgcgtaag attttcacgg atttccccat ttgcgggacc tttgaattgt
     6061 ctgccgacga tgcccatcac gtcgtcgtcg tcctgcgcca taccgtcggc gatacgctga
     6121 acgtcacgga ctgcaccggt gcgacctatg aatgtttcat cacccgcatg gaaggccatt
     6181 cggcgtacct cacgccggcg cgcaagattt ccgacgggcc gtcgtccaac ggcagggtca
     6241 tcctggcggc agggctcctc aagggcgaca agttcgactg ggtcgtccag aaggcgacgg
     6301 aactcggtgc cgggcggatc gttcctgtac aaatggagca ttgtgtagta aaattagatg
     6361 ataaacggcg gcaggcacgg ctcgaacgct ggcagcgcct ggccctggaa gcggccaagc
     6421 aatgcggccg cgacgacgta cccgacgtgg caccggtcat gagcttcacg gaactcgtcg
     6481 acgcctatgc cgacacgcgc ttcgtcatcc cctatgaaca ggagacggcg cccttgtcgg
     6541 aagcctgcca cgacgtgcgt accggcgacg tcgtcatcgc catcggtccc gaagggggct
     6601 atgccgaaaa agaaatcgcc tatgccacgc agcgtctgtc gtggtgccgc acggtctctc
     6661 tcgggccccg catcctgcgc gctgaaacgg cgtcgctggc ggccctgtct atcattatgt
     6721 acgaaagagg attcaagtaa tgccaactat cgcttttgcc actcttggct gccgtgtcaa
     6781 ccagtacgat acggacagta tgcgcggcct cttcctggct gcaggcttca cggaagccga
     6841 tttcaatggc ccggccgacg tctatgtcat caatacctgt tcggtcactc aggtcggcga
     6901 aaagaaatcg cgccagctca tccggcggac gaagaagcgc aacgatgcgg ccaaggtcat
     6961 cgtcaccggc tgctatgccc agctcgaccc ggagcttttg acgtccatgc ccgaagtcga
     7021 cgccgtcgtc gggaccaatg aacgcaagcg catcgtatcc attgtccgcg aactcctgca
     7081 cgacgaagac gacgaagcca tcacggccgt ccacgacgtg cgccgccacg atgaattcga
     7141 ggaaatcccc ctctatccgt cggcagtgga acacacacgg gccgacctga agatccagga
     7201 aggatgcaac aatttctgtt cctactgcat cattccctat acgcggggcg ctttgaaatc
     7261 gcgccagccc gacgccatcg tcgccgaagc caaccgcctg gtcgcagcgg gcttcaagga
     7321 aatcgtcctg accggcatcc acctcggcgc ctatggccgc gacctcaacg accatccgtc
     7381 gctggcccac atcctggacc gcctggtcaa ggaaaccgac gtggcccgca tccgcctggg
     7441 ttccatcgag tccctggaaa tcggcgacga catgatcgac gtcatgaaca gctcggaccg
     7501 catctgcccg catctacacc tgcccctcca gtccggttca gacccgatcc tgaaggccat
     7561 gaaccgccat tacacggtgg ctgaatatga ggacctcatc gcccgcctgc gcagccgcat
     7621 cgacggcctg actgtctcga cggacctcat cctgggcttt cccggcgaaa cggaagccct
     7681 cttcgaggac accatggcga cgctggaacg cctctctttc tcgcatatcc acgccttccc
     7741 gtattcccag cgcaagggga cgccggcagc ggatatgaag gaccaagtcg aaacggcgga
     7801 aaagaaacgg cgcgtcgaac tcgtcaatga actgtcggcc cgccagaaag cggcgctcct
     7861 gacggccctg gtcggcaaga aggcctcggt cctcatcgaa aaacaagacg gcacgacagg
     7921 cgaaggcttt acggagaact acgaacgggc cgtcgtcgaa aatcttccgc ctcatgccga
     7981 aggcaccgtc gtcgaagtca ccctcatcgg cactgacggg cagaagctgg aagcgcgttg
     8041 ttagtggctc gtggtttcgg cctgaggcct agtggctcgt ggtttcgacc tgtgagcccc
     8101 taacccctaa ccactagctg ctgactgcta acccctaaca cttgtagggg ctcgcacgtg
     8161 gcgagcccgt ccggaatatg aacgcattct gcctgcatga ttacgacggt ctggtgggcc
     8221 agaaaggctt tcaggccggc ccctacgtac acctacctaa ggaggaattt atcatggaag
     8281 actgcatttt ctgcaagatc atcgccgggg aaatcccgag cacgaaagtt tgggaagacg
     8341 ataatttctt cgccttcaag gacatcaatc cggtcgctcc ggtccatgtc ctggtcatcc
     8401 ccaagaaaca tatccagagt atcgccgact tgacgccgga cgatgtcgct gtcgctggca
     8461 agatgctcta tgccatccag aaagtcgccc aggtcatggg ccttgataaa gacggcttcc
     8521 gcgtcgtctt caacaccggt gaaaaagcag gccagacggt ccatcacatg cacgcccaca
     8581 tcttaggcgg caaagaaatg gcttggcccg gcgtatgagt gaacggcagc ggagacggaa
     8641 gccccagcgc caggtgacga ttacagcggc ggcccttacc cgcatcatcg ccatcgtcct
     8701 gctcgtcggc gtcgtcgctt tcctgatgat ccacaaaatt taatcttctt ctcataaaaa
     8761 aagacccgga agggtctttt tttcatgtaa ttttaatctt tctcgtctac gatggacgta
     8821 atcggataca ttgagaaagg ggaatcgcca tggagaagaa tctattaggc tattatggag
     8881 aacaagcggc gctggccttt gtgcgccagg aaaagggcta tcacgtgcgc tgctgtaatt
     8941 accgcaaccg cctgggagaa atcgacatca tcgccgacga cggccatacg ctggtcttca
     9001 tcgaagtcaa gacgcggacg acaggttctt acggcctgcc ctgcgaagcc gtcgagaaac
     9061 gcaagcgccg tcagatcacc cgcgtggcct cggcctatct ggcccagttc gatctttggg
     9121 agcggccatg ccggttcgac gtcatcgaag tctggccaag agataacgga cagcctggca
     9181 tccgccacat tgctcatgcc ttcctggccg aaaggcggtg acgccgtgtt cgcgcgtatc
     9241 tatggggcgg cgacactggg cctgaacggg cacgtcatcg ccgtcgaaac ggatatttcc
     9301 aacggcctgc cgtcgttcga cctggtcggc ctggcatcga cgtcggtgaa agaggccaaa
     9361 gagcgggtcc gggctgccgt caagaacagc ggctatgaat ttcccatgcg ccgcctgacg
     9421 gtcaacctgg cgccggctga cctgaagaag gacacgtccg gcctggattt gagcctggcc
     9481 gtggctatcc tggtgtcgag cggccagatt cccgaagagg cctgcgaaaa atgtctcttc
     9541 atgggcgaac tggccctgga tgggcgcatc cggccggtga acggtatcct gcccatggtc
     9601 ctggaagggg ccgcccaggg gatgaagacg gtcttcgtca gccgcgacaa tgccgccgaa
     9661 gccctgctct gtccggacct cacggtctac ggtgtcgata cgctctgcga cgtcgccgcc
     9721 tgcctgaccg gtgaaaagcc gctggacgcg gcccggccgg aacccttggc gccggaccag
     9781 ctggactatg acgtcgactt cgccgaagtc cagggccagg ccgaagccaa gcgggtcctg
     9841 gaaatcgccg cagccggcgg ccataacgtc ctcatgatcg gcccgccggg aagcggcaag
     9901 accatgctgg ccaagcgcat cccgactatc ctgccgccca tgagcgaagc cgaggccctg
     9961 gaagtgacca agatatacag cgtcgccggc cttttgggca aggggcggct cctgcagcag
    10021 cggcctttcc gcagccctca tcacaccatt tccatggccg gcctcgtcgg cggcgggtcc
    10081 atccccaagc cgggcgaagt gaccctcagc catcacggcg tcctcttcct cgacgaactg
    10141 ccggagttcc cccggtcggt tctggaagtc ctgcgccagc ccttggaaga cggcgtcgtc
    10201 cacattgccc gcgtccaggc ggccctgtcc tatccgtcgc gcttcgtcct ggtggcggcc
    10261 atgaatccct gtccctgcgg ccgctacggc ttcgacgaag gctgtacctg ttcggacagc
    10321 gagatacgcc gctataccca taagatttcc ggccctctcc tggaccgcat cgacctccac
    10381 gtccgcgtcg aacggccggc ctactgggaa ctgacggccc ggcggcgggc cgaaccgtcg
    10441 tcggccatcc gctcgcgggt cctgtcggcg cgccatcgcc agcaagagcg cctggctcct
    10501 tatggctgta cctgcaacgc ccatatgggc caccgggaga tacgggccac gtgccagctg
    10561 accgaggcgg cccagcagtt gttgcgcgag gccttcgacg ccctgcgcct cagtgcccgc
    10621 agttacgacc gcatcatcaa agtgtcccag accattgccg acctggccaa agaaccggtc
    10681 atcgccaagg atcacgtggc cgaagccatc agttaccgca ataagatgca ccagccatga
    10741 aggggggatg aaggtggaac aggaaacgat gtacgcagcg gccctccagt ccctcaaggg
    10801 ctgcggcagc cgccgcctgc aggccctcct cgacgtctgc cgctcgccca gccaggcctg
    10861 gggtgccgtc tgggatgaag acctgcggcg ccggacgggg attccggcca agattttttc
    10921 tcagctcagg caagaacgag atgtttttga ttgggatacc ttccagcgca ggctgtcttt
    10981 ttatggcgtt cggcccgtcg ccctgtggga cgacgattac cccagctggc tgcccttcat
    11041 cgcccagccg ccgctggtcc tcttttgcca ggggacgatg gagcgcgaca gcagcagtat
    11101 cgccatcgtc ggctcccgca aggccagccc ctatggcctg aatgccgccg agaccctggc
    11161 cgctgagctg gcggcccagg gcctcaccat cgtcagcggc ggtgccaggg gcatcgatac
    11221 gcgggcccat cggggcgccc tgaaaaggaa ggggcggact gtcgtcgtcg ccgccaacgg
    11281 cctggaccgg acctatccca gggagaacaa ggccctgttc cggcaggtcg tcgatagcgg
    11341 cggcgcagtc atctcggaat atgccttcgg cgtcgagccg ctatcccgga atttcccggc
    11401 ccgcaaccgc atcatcgccg gcatggcagc ggcgacgatc gtcgtcgaag cggccctgcg
    11461 cagcggctcc ctgattacgg ctgacctggc cctcgacgaa gggcgcgacg tctttgccat
    11521 gccgggcagt gtcttttccg agaccagccg ggggacgaac catctcctcc ggctcggcgc
    11581 cattcccctg acctgtgctg acgatgtggt ccgggaattc cgccgccgcg gctggcaggg
    11641 gccggagacg gcctgccatg aggaaggacc cagcctgagt ccggccgaac aggccgtcat
    11701 cgacgccatc cccttcgacc gggcggcgcc ggtgagcgag ctcctggaaa agacgggcct
    11761 cgccgtcgcc gacctgctgc ccatcctcct ggccctgcag atgaagaaag ccgccgagga
    11821 actccccggc ggctatatcc gctgtgctgc ggccttttct gccctataag gcggtgatat
    11881 acgcttgggc tttgtcgacg gagacgacgt cgatccagcc gaaggggacc agggcatgac
    11941 agcaggcaaa gagggtatga tggtctgtga tgaattcttt cagggcggcc atggtctggc
    12001 tgctgccttc gacgcggcca tagccggcga cggctgcgtc gatgagggca ttgtccggcc
    12061 attcctcgcg gaagcagctg tgcaggaaga ggaacaggtc caggacgggc gccggcgcgt
    12121 cgacgaattt cttttcattt tcccagtcga ggaaggtaat cgtcccggtt tccttatcgt
    12181 aggcaatgtc ccgcagggcc ggccgaccgt ggtgcaggcc ggcggcgtgg agctgagcca
    12241 gtccctggcc ggctttttca aaggcatcgt ggcggacggc ggcgtattcc gcttccttgg
    12301 ctacgccctg caaggtcttg ccggcagctt ccatgacgaa gaagtcttca tccaggagga
    12361 cgaccttcgg cgccagggga aggcgggcgt tgacggtcat gatcttgtag gcttcgcacc
    12421 aaaaggcggc cgacggattc tgtttggcaa aggcattgcg gccattgccc atgcggcgct
    12481 tgacgaagta cgttttattt tcaaaagaaa gtgataaaat ccgcttgtcc ggcgcttttg
    12541 tgatggcaga ttgaacggat tttaagagag acctgttgaa caagaaagtc acacctttca
    12601 aaattttaag atataatttc aatatttaca gtttctacac ataagaacgt gcctacatta
    12661 taacaaattt tgtcagtaaa ataaatggcc tggcccttga aaataaggca ttttcctgcg
    12721 aactcccctc aaaatagacc atcttgtaaa caatatgtta tattgaattt gataaacaat
    12781 attgacacgt gaaagaagac actttataat aatagacgta aatgaatatc aattttttca
    12841 agggagatgt gtcgtatgtt tcagaataca gatttttgga ttggcttagg tatcggtgtc
    12901 gtcgctggtg ttttcggtta taaactcatg tccgaacggg aacagcagct cctggctatg
    12961 cagcagcccg ctgccggtgc gatgaccgct tcggtacccc tggctgaatt gcagcgtcag
    13021 aaagaagaat tggaagacat gattgcagct caggaagccg cacaggcgaa ataagtttgt
    13081 agggaaggcc tttgcggttt cagcggaagc ctttaaattt aaagccatct gctaacaact
    13141 aaccactagc cactaaccat ttaaaagggg ctgtcgtacg aagatttttg ccatcatgca
    13201 aggccctatt tactttatag tttgtagtac ggagtttgta gtttgtagaa gctggcttta
    13261 aatttttacc atttgctgca aactaaaaac tacaaactta aaactcaaaa agaggct
//
LOCUS       DAWABZ010000043        13249 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_1285_length_13249_cov_3.227603, whole genome shotgun sequence.
ACCESSION   DAWABZ010000043 DAWABZ010000000
VERSION     DAWABZ010000043.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 13249)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13249)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13249
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1285_length_13249_cov_3.227603"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8360..8369
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3199 a         3682 c         3555 g         2803 t
ORIGIN      
        1 ccaaccactt ccgcctgaac tactccaaca tgccggaaga ccgcatcgtc gaaggcatta
       61 aacgtttggc tgaagttttg aaagaaaaaa tcggtaaata attaaatttt aaggaaacaa
      121 ggcgctctct cgtgagggcg tcttttcttt tgcaaatttt tttaaaaatt ttttacagtc
      181 attctttaat tttttttaag ctggatattt actattatca gtcattctcg ttatttacat
      241 ttggtttata aaggttgtac gaaacgtaac atatttataa cgcaaatgtt taattgcata
      301 aaagtaattt tgatgatact atatatacat agataaacgc gtgatgtaca taacctgaag
      361 gagtgattga tatgcatttt tccgacttag ctatgaattt gaaagcctct gaaatccgtg
      421 aactcttgaa actgacgacc atgccggaag tcatttcctt tgctggcggc ctgccggcac
      481 cggaactctt cccgacggaa cagctgaaga aagttgacga cgccgtcctg accaaagaag
      541 gccgcgaagc cttacagtac ggcacgaccg aagggtacaa ccccctgcgc aaacagattg
      601 cagcccgcat gaaacaggct ttcatggtcg actgcgatat cgataacatc actattacgt
      661 ctgggtccca gcagggcctg tccatgctgg cccagatttt cattaacacg ggcgacatcg
      721 tcctcgtcga aagcccgacc tacctcggcg ccctcaatgc ctttaagctc tgcaaaccgg
      781 aattcgttga agtgccgacg gacgaaaagg gcattatccc ggaagaattg aaaaagattc
      841 tggaaaaata cggcgaccgc gtccgcatga tgtacgtcat cccggaattc cagaacccga
      901 cgggcatcac ttggcccgta ggacggcgca aggctttcat ggacctcatc aaccagtatg
      961 acttcccggt cctcgaagac gacccctatg gcgaactgcg ctatgacggc gacaaggtac
     1021 cgtccctgaa atcgatggat acgaaaggaa atatcatttt tctcggttct ttctcgaaaa
     1081 tccttatgcc gggccttcgt attgcctgga tggtagccga cccggtcatc atcgataaag
     1141 tcgtcaagct gaaacaggcc gtcgacctcc agtcgtcgtc cttcggccag cgtcagacct
     1201 cgtactacat cgatatgttc gacctcgatg aacacgtcgc caagatcaag gccctctata
     1261 agaaacgccg cgacctgatg tgcgaatcga tgaagaaata cttccctgaa ggcatcacct
     1321 ttacataccc cgaaggcggc ctgttcacgt gggtaacgct gccggaaggc atggacgcga
     1381 aagaactcat gccgaaagtc ctggctaaga acgttgccta cgtgccgggc ggtcccttct
     1441 acccccacgg cggccatgcc aaccacttcc gcctgaacta ctccaacatg ccggaagacc
     1501 gcatcgtcga aggtatcaaa cgcctggccg acgtcttgaa agaagaattg gcaaaataag
     1561 gctaaagatg atatgatata aaggagctgt cgcttacgac agctcctttt tgagcattaa
     1621 gtttgtagtt tgtagtttgt agtttttagt gaatggctaa aaaatttaaa accaactgct
     1681 acaaactaca atctctattc tataaactca aattcatcag cagtaaagct gagttgttag
     1741 tctttcgtag atgggttgat tatcttgatg gaactcgaat ctcttttgaa acaatatttt
     1801 ggctatacgt cgttccggcc gggccagcat gaggtcatcc agacccttct cgatggccgc
     1861 gactgcctgg ccatcatgcc gacaggggca gggaagtcga tatgcttcca gctgccggcc
     1921 ctgatgatgc ccggcgtgac gttggtcatt tcgccgctca tttccctcat gaaggaccag
     1981 gtcgacagcc tggtcaacca ggagataccg gcgacgtata tcaacagcca gtgtactttt
     2041 gaagaggcca aggcccgctt cgccgccatc cgcgccggcc gggtcaaact ggtctatatt
     2101 tcgccggaac gcctggaaaa cgaattcttt acgtccttca tgcagagttt gcccatttcc
     2161 atgttcatca tcgacgaagc acactgcgta tcccagtggg gccatgattt ccggcccagc
     2221 tactgcgcca tcaaggactg gattgcggcc ctgcccgagc ggcctgtcgt cggggccttc
     2281 acggcgacgg cgacggaaaa ggtcaaagag gacatgatga acctcctggg cctggaaaag
     2341 gaacgcatct tcatcggcgg cttcgaccgg ccgaatctgt atttccgcgt cgtccggatg
     2401 aaccgcaagc tcgacttcgc cttgtcctac gtccagcagc atagggaaga cagcggcatc
     2461 atctatgcgg cgacgcgcaa ggaagtggac cgcgtctatg aagagctgac gcggcggggc
     2521 atccgggccg gccgctatca tgccggcctc agcgacgaca tgcgccggac catgcaggac
     2581 gcctttacgt atgaccggct ccaggtcatc gtcgctacca acgctttcgg catgggcatc
     2641 gacaagagca acgtccgcta cgtcatccat taccagatgc ccaagaacat tgaaagctac
     2701 taccaggaag cgggccgggc cggccgcgat ggtgcgccgg gggaatgtat cctcctcttc
     2761 agccgccagg acatcatgat ccagaagttc ctcatcgaac agtccgtcca cgatccgcag
     2821 cagcaggccg tcgaattccg cctgctcaac gccatggtcc gttactgcga aggcaatcac
     2881 tgcctgcgcc attatatcct gacctatttc ggcgaacatc catcgtggca gcgctgcgag
     2941 aaatgcggca actgcgacca ggaaacggtc gaagaagaca tgaccgaaca ggtccgcagc
     3001 atctgcctct gcgtcgacga attgaaaggg cgcttcggca tgaccatggt ctgcgacatc
     3061 ctcaagggaa gccagaacgc caaggtccgc cgctatggct ttgaacataa ttcggccttc
     3121 ggcatgctcg gcgatttcac tttgtccgaa gtccgcgaca tggtccgcca gtgcatcgac
     3181 gacggctatc tcgaccagtc cgatgggaaa tatcccgtcg tctccctgac ggccgacgga
     3241 cggcaggccc tcagcggcca tcagcgcatt gtccagcaga aagtcgtttc agctgaaccc
     3301 gtgccggaac tgcccaagcg ccagcagaag cgccgcgccg gggccatcga cgaagacgcc
     3361 ttgcggcccc tgttcgatac cctgcgggca gtccgcctgg aactggccaa ggacgagcat
     3421 attccgccgt tcgtcatctt ttccgatgcc acgttgtggg atatggccgc tctcaagccg
     3481 gacagtctgg aagccatgag cgacatcaag ggcgtaggca gcttcaagct ccataaatat
     3541 ggccgccagt tcgtcagcgc catccagtct tatatagata accattgatt ttgaggtgac
     3601 atacatgagc attgccgata cacagtatct gaatatcgtc gaaaatatcc tcgaccacgg
     3661 ctatttcgac aacaaccgca cgggcgtgcg gacctataaa ctgccccacc agatcatgca
     3721 gttcgatttg gaaaaagaat tccccatctt gacgacgaag ttcgtcgcct tcaagacggc
     3781 cgttaaagaa atcctctgga tctggcagct ccagagcaac gacgtgcggg aactgcagaa
     3841 gatgaactgc cacgtctggg acgaatggat gcggcccgac ggcaccattg gcaaggccta
     3901 tggctatcag atccgcaagt acaaacaggt cgacaagctc ctggaaacgc tgaagacgga
     3961 tcctcagaac cggcgcatga tcgtatccct gtggaacatc gaagacctgc cggacatggc
     4021 ccttcagccc tgtgcctacc agaccttgtg ggatgtcact gacggccgcc tgaactgcat
     4081 gctcatccag cgcagcggcg acatgggcct gggcgtcccc ttcaacatga cccagtatgc
     4141 cgtcctcatc catctcatcg cccagtccgc cggcctgcgt ccgggcctgt tcacgcacgt
     4201 catcaacaac gcccacgtct atgagaacca cgtccaggcc atgaagaccc agctggcccg
     4261 ccgtgaccag gcctttccgg caccgaagct ggtcctcaat cccgatgtcc acgacttcta
     4321 tgatttcacc atcgacgaca tcgccctgga caattaccgg cacttgggta aactgcccat
     4381 ggaggtggcc gtctgatgat ccatctcatt gcagccgtcg accggaacgg cggtattggc
     4441 aaggacaacg acctcttgtg ccatatttcg gccgacttaa aacgttttaa atccctcacc
     4501 atgggccata cgatgctcat ggggcgcaag actttcgaaa gcctgcccgg cgtcctgccc
     4561 ggccggccgc actgggtcct gacccatcaa aaggattaca gcgtcagcca tgacgccgtc
     4621 actatcttcc acagtgtcga agccatgcag caggccatgg aagccgggga ggactatttc
     4681 gtcatcggcg gcgcttccat gtacgaagcc tttctgcccc tggccgacag cctgtacctg
     4741 acggaaatcg acgaagcctt cgacggcgcc gacactttct tcccggcctg gaaaaaggac
     4801 gagtgggagg aacaaaaccg ggaattgcac cgtccagacg gacgggaaaa atattctttt
     4861 tctttcgtcc actatgtccg ccgtttcgat tgacattgtt aatccgttat gttatactaa
     4921 cgtaagagct acaagatagc tggatttgtt tttttaggag gattttgtta tgcacggtaa
     4981 agtaaaatgg tttagcgcag aaaaaggtta tggcttcatc gaaagagaag atggcggcga
     5041 cgttttcgtt cacttctccg ctatccagga agatggcttc aaatccttga ccgaaggtca
     5101 ggaagttgaa ttcgacatcg ttgaaggtgc gcgcggaccg caggcagcta acatagttaa
     5161 atgctaatcg gaaagtaaaa tagacctttt aaggggctca caggaaggct tcggccagcc
     5221 tgcgcggccc ttttttttgc accttgtata tgtataaaaa cacgaaaaag tttgcataaa
     5281 tacttgcaat attcatacgg ctgtgctatg atatatccat cgaacaagtt ctggtcttaa
     5341 ctattggagg atggatatac atgaaacatg caaacagctt cgcagattat acaaccgatg
     5401 aattgaaagg cattttattg ctggccgaaa agatcaagca gcatcaagat gattacggcc
     5461 atatcctgga tggcaagaaa ttgtacacgc tctttgaaaa gacgtcgaac cggacgtacc
     5521 tttccttttc catcggcatg gaagaattag gcggcaaagc ctacaaccag ctgtggaagg
     5581 acagcaattt cactatcggc gacctcatga gtgaagtcaa atacgtctgc cgcaacgtcg
     5641 actgcatcat gggccggttc aagaaagacg aaacgacgga aggcttcatg aagaacgcca
     5701 cggtcccggt catcaacggc tgtgacaata cgttccaccc gacgcaggcc ctggctgaca
     5761 tgctgacgct ctacgaaaag accggccatt ttgaagccaa ggtcctctac atcggcgcca
     5821 agaacaatac gtataactcg ctcagcgaaa tcgtcaccaa gatgggcggc atgatgtacg
     5881 gcctgacgcc gttcagtcag attaccaatg tgccggccga tttctacgac aaactgacgg
     5941 ctacgggcca ttataaggaa ctgccgacag acatttccaa agaagacctg aagaaagtcg
     6001 tcgccgacgt ggactgcgtc tataccgaca cctgggtcga catggaattc ttcaccaatc
     6061 cggactatgc cgaaaagaag aaccaaatca tcaacatgat gatgccttat cagatcgacg
     6121 ccgccctcat ggaagggacg gataccatgg tcttccacga catgccgatt catccgggct
     6181 atgaaatcac gcaggacgtt atggaaaaac acctggaaac catcctcgac gaagccgaaa
     6241 acagacgcca cgccgaaaaa ggcctgttag tctacctcat cacggggaaa ttggatgttt
     6301 aggtcatatg aaggtttctg ccatcatgca gggattcttt cgctctatag tttgtagtac
     6361 ggagtttgta gttttcagcg aaacctttaa atttaaaacc atctactaac aactaactgc
     6421 tagccactaa taacttaaaa gggattgtcg catgtgcgac aatccctttt ttcatcccaa
     6481 tcccaggcag accaggggca gggtgacgat ggagcagagg gtcgtgacca ggatcatgcc
     6541 ggcggcgtag ttaccggctg agtgctggtt ctgggccagc atgaccacgg cggccatgct
     6601 gggcagggcc gacaggaggc tcatggtgac cgtcacgtcg tggacgacgc ccagggtttg
     6661 gcagagcatg tagaagaggg gcgggaagag gcacatcttg atggcgacgg cgccgtagaa
     6721 ctctttcttg cggatgtagc ggggaatgtc gatgtagcag aacaggccgc ccatgtaaat
     6781 catggccagc ggtgtcgtca aggcgccggc tttggtcagg gccgtattga ccggagccgg
     6841 gagctcgatg cccgtaaaga tgccgacgat ggccaggacg atggcgatgg tcgccgggtt
     6901 gaccatcttc tgcaggcggc tggcgatgga catggcattt tcccggtcca ccggtgccgt
     6961 caggttgaca cccagcgtcc agagcaggag ctggtcgatg accgtgaaca gggcgatgta
     7021 gagcatgcct tgttccggga agacggcggc gacgatgggg atgcccatga agccgatgtt
     7081 gccgaacatg gcacaggccc ggtagacgtg attttcattg cctttcaggc cgaagaggcg
     7141 tgccaaaaac agacagagca ggtataataa gaagtacatc aggaccgtca cggccaggac
     7201 cggcagggcg ctgatgaact gctggcgcgt cgccccgttg agggtgttgg agaataagag
     7261 caggggcagg gaaatcttgg tgatgaaccg cgagaagacg cccagggagc ggctgcccag
     7321 gacgcgcatg cgcacggcgg cgatgccgat gaggatatag atgacgaaac cgatgatttg
     7381 cgataagaca atggaaaaat aggccattgg ccagcctcct ttctaaaacg tccccatttt
     7441 atcatgctgg tggcggcatg actaatgaaa cctttgacca tggccatgaa aaaaagtgat
     7501 ggataggaag accgattatg gaccttgcga aaatacaata tttcctggaa gcagcacggc
     7561 tgacgaattt cacccaggcc gccaggaatt gccacatcgc ccagacgacg atgacgaaat
     7621 acattaccca gctggaaaag gaattgggct gccgcctctt ccgccgggag caccgcggtg
     7681 tctcgctgac accggaagga cggcacttct atgaaggcat ggcgaagatc tgccaggaat
     7741 atcaggacct gcgcgactca ttgcgcctga aacggcggaa aggcctctac ctgggcattg
     7801 ccatgcagga atacatcgaa gtcctgtggc tgcgccgctt tgaagaagcc tttcccgatt
     7861 gtaagctcta tttcagtttt cacgacatcg aagaactgga atagagcctg gagaacggga
     7921 ctttggatgg cttcctcttt tccgatgccc aatccctgat gggccgtttc acctgccagc
     7981 gcctgtttgc catccgccag tccttcatct gttccaagcc cctcctcgaa cgctacggta
     8041 cggtaggcga catcatcgct cacagccact ttgtgaccaa gtccaacagc ccggcctatg
     8101 ccgccaagat acgccagcac tgccgccacg ccttccatca ggagttcctg tcggtacacc
     8161 tgtgcaagac tctgtcggaa cagttgctga cggtcagcct gtcccagggc tttgccatcc
     8221 tgcccatgcc cgaaagcgat gcctatcccg gcctgagcgt catccccctg ggcgacgact
     8281 ttacagaaca tgccgtcctg gcttatcgcc aggacggcat gacagacgag ctgaaaaagt
     8341 ttttatccct ttttgccggn nnnnnnnnna tgccataatt cgctgagggc gacgccgccg
     8401 aggatgcagg cagcaccgat ccattgcagg aaggtcaggc tttcgccgag gaggatggcc
     8461 gacagggcca gggccgtaaa gggttccatg tagccgcaga tagagacgct ctgggccgac
     8521 aggcgctgga cgccggaaaa atagagatag tagccgaggc ccgtgttgac gatgcccagg
     8581 cagaccatgc aggcgatgtt ggtcatatcc aggctgaccg gtgccgtcgg catggacaaa
     8641 gtgacggcgg cgacgacgag cgacgccacc aggagctggc aggccgggga gaggatgccg
     8701 ccgatgccgc tgaccttttt catggcaatg accagcaggg caaagcagac ggcgctcagg
     8761 aaaccgcaga ccaggcccca cgacaggccg tgcgcctgaa cgtcggcgcc attgatgcag
     8821 aggatgccga ccgtaacgat gaccatggcg cttactttcg ggaccgtaaa gggctcatgg
     8881 aagacgaagt acgacaggat cataatcagg atgggcccgc agtagcacat gagggttgcc
     8941 aggctgacgc cgatatgctg gtaggcttcg tagaggaaga gccagttccc ggacaggaag
     9001 aggcccgaca ggaccagcca gaccagggac gtgcgggacg cgcggcgcag gctgccccat
     9061 tcgcggcggg ccgtcgccag gatgagcagg aagaaaccgc ccaggaccgt acgggtcagg
     9121 acgatttcat aactcgacag gggaatatgg ctggcgatga tgccgttcgt gccgaaaatg
     9181 acagcagcca ggatgaattt gatgtacgca tttttcatga gcgtccctcc ttcgcattga
     9241 taagtttact ataccatatc tgacgccatc tgtctgtatg gctgacgcca tgaaaaaaag
     9301 tgattgaaaa tgacgcgtct ttcatttcac aacgatcttt atctatgata cgattccggt
     9361 agtgaatgag aaaacattcc gtgaacagga ggaactatca tgaaaatatt tcatatcgat
     9421 gaagacgtct ttcgcatgct tcccgactat tatgtcggag tcgtcgccgc tgaaggcctg
     9481 gtgaaccgtc aggacaatcc ggccgttgcc gaccggctgg accaggccat tgctgccttt
     9541 gccgatgatt tccagggtat caacctgaaa gaagacccgc atatcgtccc gtaccgcaat
     9601 gccttccgcc tcatgggcat caaccccaat aaatatcttt gctctatcga agcgctggcc
     9661 aaacgggtgc agaagaagaa ggccctgccc catatcaatc cggccgtcga cctgggcaac
     9721 gccatttcct tggaagaacg cctgcccatc ggcgcccacg atgttgggaa tttctgcgat
     9781 ggccgcatgg aagtccgcct ggctgccgaa ggtgatactt ttttaccgat gggcggtggc
     9841 gagctggaaa agccggacga acgggaactg gtctatgtca gcggccatac cgtaaagacc
     9901 cggcgctgga cctggcgcca gagcgatgat gggaaaatca gcgaagatac gcaggccatc
     9961 ttgttcccta tcgacgggtt ttacggagtc aatgaaaaag ccgtcgccga agctgtccag
    10021 aagttgtccg acgccgtctg ccaggccttt ggctgtatga cccatacggg catcatcgac
    10081 cgggaccatc cgaccttttc ctggtaagcg cttttttgtt gacagccatt ttgttttgtg
    10141 ataaagtgaa gctcgtagcc tcagcgaggc tgctgtaaaa ttaaatcaga ttaccgccgg
    10201 gtacgcttgt actattccgt taggcctttg aaggaatagt atgggcgtac tttttttcgg
    10261 cggaaatgaa aaggagcgat tcgtatgtct tggatgtatt tattgattgc cggtgtcttt
    10321 gaaatgggct gggccattgg cctcaagtat acccagggat tttcacaact cgtaccgagt
    10381 atcgtcaccc tggccagcat ggcagcaagc ctggttttcc tgagcctggc cctgcgccac
    10441 ctgcccattg gcattgccta tgccgtctgg acgggaatcg gcatcgtcgg tacggccgta
    10501 gccgggattt tcctcttcca tgaatcgacg acgccctggc aggtcctgtc cctggtactg
    10561 atcatcgccg gtatcgccgg cctgcgcctg accagcgcgg cctgaagggc tgttgcatga
    10621 agacttctgt catgatttgg gaaacctctc agtcagctgc gctgacagct ctcctatcag
    10681 gagagccttt gcgagccaat agccgcgagc cacgagccgc gagccactag ccactagcca
    10741 ctagccacga aatatatggt ataatagggg aaaacagaaa aggggaactg ccatgagtat
    10801 gcatcatgaa gtaggcatta cttgtccgaa atgccagaag gccggcgtct tcacggtatg
    10861 ggacagcatc aatacgaaac tggatccgga catgaaggac cgcgtcaagt cgctggaagc
    10921 ttttcgctac cactgcggcc actgcgggac agacgtccag gtcgattacg gcttcttgta
    10981 tcacgacatg gaccatcatt tcatggtctt ctacgcgccg acggataagg accaggaaaa
    11041 tgccgaccgc gatatggccg acggccagga taaattcaag gccatggtcg agaaggcagg
    11101 ctatgtcttc cgcctggtcc gcagcaaagc cgatttgttg gaaaaaatcg ccatctttga
    11161 agccggtctc gacgaccgcc tgatggaatt gaccaaggcc gctgccctgg ctcaggtcgg
    11221 ggaaaaggat aaggatttca ccgttacaga aagccatttc ggcacggctg acgacggctc
    11281 cctgcgcctg aatctaatcg acgggaccca tggccagcag gcctatgtcg actttgccaa
    11341 cggtctgggc cagctctatg gccagtttga aaagaacttt gccgccgacc tgccggcaga
    11401 agatgccgtc ctggcccgca tcgacgcggg atggactcag cagttcttga ataaatattt
    11461 atataattag gggagggatt tccatgtcga aactcgtcgt cgtcatccaa tgcgatatcg
    11521 tcaccaaacg ctgtatcggc tatgcctgca tgaacacctt tgtaaaccgc aaagatactt
    11581 ttgccgatta tccgcccgat acgcgcttcc tcatgatgac ctgcggcggc tgctgcgggg
    11641 cagggctctc ggcgaaactg gaaaatctgg gcaggaagct gaagaaattc ggcgatgcca
    11701 aggaaaatgt gaccatccac ttcgcgtcgt gcatcgtgtc cgacagctat caccggccgc
    11761 cctgtccttt ccgcaattat atggcccaga tcatcgaacg caaaggctat cccctggtcc
    11821 acgggaccta tatttcccgc atgaccgaag aaaaacggaa gcaagggact tatcatgcct
    11881 tataaaaagg ggaggagcct cgtcgcagcc gtcctgatgg tcagccttct tggcggggga
    11941 ccggtcttcc tgccactgcc cggcgtggct gctgcaggaa atgccgtcgt cgataagaaa
    12001 gctatgaaac aggccgattg gcagctggct gaggcctacg gccggcccat gctcgacgtc
    12061 ccggccggtg ccgatacgat tatgggtccg gcgacgatcc cggcccgcca gatggtccat
    12121 ttcatccgcc agcgcaatcc ccatcctaag ctgaatgcgc cgctggaaga ggtcgtccag
    12181 gcctattatg acgaagccgg ccgcgaaggc atccggcccg acgtggccct ctgccaggcc
    12241 ctgaaggaaa caggctactt tgcctatggc ggtgacgtgt cgccagacca gaacaatttc
    12301 tgcggcctcg gcgcgacggg aaataaagtc gccggtgccc gtttcgctac gcctcagctc
    12361 ggcgtgcgcg cccatatcca gcatctcctg gcttatacca gcacagagcg gcccaagacg
    12421 gccatcgtcg atccccgcta tgaactgctg gccgaaaaac atcccgaact ctatggtaag
    12481 gtcgattcct ggacgggcct caatggccgc tgggccgtgc cggggaagca ttacggccag
    12541 gaaatcctct ggatgtggac cgaagcccag acgccggacg gtacgaatga cagcctgatt
    12601 acgggcttcg aaagagtctt tgcccatccc gacgacgccc aggcctatct ctaccggggc
    12661 atcctcttct tcaaccacca ggactactgg ctggccgaac gggacttccg gcgcgccctg
    12721 gaactggaca agacgtcacc ggcggcctgg tacgacctgg ctttgacgca acaaaaaaga
    12781 ggggagcccg aagcttccct caccagttat gaccaggcca tcgcctgccg gcccgaatat
    12841 ctgcaggcct ggtataaccg cggcctggcc gccatggacc tgcaccggga cgacgaagcc
    12901 ctgcggtcct tccaggaaat cctgcgcttt tcgccgcaat cggccgatgc ggccaacgcc
    12961 atggccatcg tctatctccg ccagggaaaa tatgacgacg ccggctcgtg gctggaaaag
    13021 gccggccaga tcaattcggc caatcccagg gtccaggcca atcgccagca gttccaagcc
    13081 taccggcggt agctgtattt gaatttcatc aatttatcca tcaaatattc caagtgactc
    13141 ttttctgcct tcatcggtcc atcggtcgtc tgtaccaggt gggccgtttc ttttttctga
    13201 ttgaattcca cgtcatagcc atgggtcacc atcaaggctt gttccaatc
//
LOCUS       DAWABZ010000044        12386 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_1396_length_12386_cov_3.036656, whole genome shotgun sequence.
ACCESSION   DAWABZ010000044 DAWABZ010000000
VERSION     DAWABZ010000044.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 12386)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12386)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12386
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1396_length_12386_cov_3.036656"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    995..1004
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1652..1661
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5163..5172
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6057..6066
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10978..10987
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2794 a         3470 c         3193 g         2879 t
ORIGIN      
        1 aaccacaact ttacataccg tcagaaggat aaaggctggc aggtcatact ttcatataag
       61 gacggtgata aatggcgtca gaagagcaaa caggggctaa agactaagca acaggcaaag
      121 gctgctggcg acaaactttt atctgagctt caaagaacat ttgtaccctc atcgaatgag
      181 ctggcaggaa ccacgctccg taactttgtc cccgtcgtca tcaaggataa ggcattgacg
      241 ccatcgacaa aatatgcatt cagccgcgtg ccgattttct ttgctgatat tgctgataag
      301 gtcatctccc agataacaac agccgacata ctgcatgtcc tgtctacaaa gaagggcctg
      361 gcaaatagta cgatacgtca ccggctgacc ttgttgaaca ccgtattcaa ccacgctatc
      421 caggtgtacg gcattgcgat aaggaatccg gtgcaggccg tgccgcgtgt aaagctgccg
      481 aaacaatcct gcatcaaggc tatttcccat gatgatttca caaggctgat gaaaagcagc
      541 ggcggccgga attgcgataa ttacaaaatt tgttgccaga ttgcctatta tacgggaatg
      601 cgattcggtg aaatggtcgg gctgatatgg gatgatattt cctttgataa cagacagata
      661 accgttaaac gacaggtaaa ggcatatcgc catgacggca taatcacgta cgggccaggg
      721 cctttaaaga cgacgaattc ctaccggacg attcccgtcc ccggagtcct gcttgacgca
      781 ctgaaaaact ggaagcagaa aagcgcgtca gaatcggttc taggcttaat gagttccaac
      841 acgcaattaa tcaaccgttg gataaagaaa acgctgccaa atacgagtat acatgatttt
      901 aggcacacat acgccacgaa cttactggca aacggcgtag acattcaaac cgtcgccgca
      961 ctatgtgggg actccgtggg aaccatcatt tccannnnnn nnnnaagaga tgagaaagaa
     1021 ggccgcccaa aacgtcgata ccatctttac aaaatgattt ttgacgaatt tttgacggcc
     1081 ctctatcggg atgctatgaa acggcactat taagccattc ttcgtttgtg cggttataac
     1141 aataaattat atcatagaat atccaaattt tatagtcttt tagtcgtcaa actcctattt
     1201 ttccgggctg ccgttgacgg cctgccagac ctgggcgacg aacttgcccg ctgctgggga
     1261 catttcctgg atgtctggga cggcgatgcc cagcgaaatc gactgggccg gggccagagg
     1321 cctaagttcg acgcggccag cccacttctc cgtcgtcagg cggttgttga cgctgattcc
     1381 caaaccctct tcgaccatgc agtacgccgt atacgggtcg gtcgtcgtga agcggacatc
     1441 gggctggacc tgattggctg ccaggaagcg gtcgatatcc gtatcgtgat ccggcagagg
     1501 gatgataaaa ggcgccccgt tcagccattc tacgggaatc tccttttccg ccgtccaggg
     1561 atgatccggc ggcagccaga cgacgagctc atcgtccttc aagggaatcc agtcataggc
     1621 cacatccgct ccgggcttgg cggccagaca gnnnnnnnnn ngccaattcc tgattcgtcc
     1681 cttctaacaa atggacttcc accttgggat actgggcctg gaaacggcgc aaaatcttcg
     1741 gcagccacgc cgcggcggca ctataataac tgccgatatt gaggaccccg gccaggacgc
     1801 cttgaatggc cgcccccaag tcggcggcct tttgcgaagc cctgacgact tcctgaaaga
     1861 ccggcaccat ctgctggcca ttggccgacg gccggacgcc ctgcttgttg cgcacgagca
     1921 gagggaagcc gatttccttc tccagcgaac ggatcatacg ggtcatcccc gactgagtat
     1981 accccagttc cttgccggcc gccgtcagac tgccatattg aatcgccagg agcaaagcct
     2041 gacattttat actatccata tcacttattc acccatttcg tcatgacata catgctaaat
     2101 caccgtttgt gtcatatctt attataccgt ataataaggc catccgtaaa ggattagacc
     2161 caaatgagga cccattatga ctactttatt acccttcgcc tcagactata tggaaggcgc
     2221 atacccggaa taaccatgaa tttcacagct caaagtggat acggtactga ttattcgttc
     2281 atcgaaattc ggtcctgtcg gccctaacgg ttcaaaaagg ctctgggacg gcccctatgc
     2341 acgaggccgc tgtccgtgta ttttttcggt ccgcaggacc gcggcggcca gcgaactgcc
     2401 tcctaataat tcactgcgtt ttgttgaaac atataacgtt taacgtcaat ttacttgaaa
     2461 ttttttgcga aaatcatttg acaaagtccg tttttgatgg tagacttacg tcaacaactt
     2521 gatagtgttg caatgaagag aagagtacac gataggaatt ggcacagaga gttcccattt
     2581 ggtgaaaggg aatgcaaggg tcatcgtgga agatggactc cgagcagatt ggtcgaacag
     2641 ttaggccgtc cgggcgtgcc cgttacagca ctatggtatt caaggctcat ttgcctgcgt
     2701 acctgaagag gctcatcctg tgagggatga gtaaaattgg gtggtatcac ggtgcttccg
     2761 tcccttactt gggacggaag ctttttttat tacaaggctt tcaagtttat attacattat
     2821 atatttttta atattagggg gaaatgtatt atgagcgaaa acacaaacta caaattcgaa
     2881 acattacagc agcatgcagg tcaggtaccg gatccggcaa cgggtgcccg cgccgttcct
     2941 atttaccaga ccacatctta tgtctttaaa gatacacagc aggccgaaga ccgcttcgct
     3001 ttgaaagacg ctggctacat ctacagccgt ctgaccaacc cgacgcagga cgtcctggaa
     3061 aaacgtatcg cagccctcga aggcggcaca gccggtttgg ctacagccag cggcgcttcg
     3121 gctatcgcct actccatcat caacctcgcc ggtgctggtg acgaaatcat cgctgctccg
     3181 accttgtacg gcggcacagt tgaactgttc agcgaaacct tcaaacacct cggcattacg
     3241 acgcacttcg ccgatcccga cgacccgcag tcctttgccg aatacatcaa cgacaagacc
     3301 aaagccattt acctcgaaag cctcggcaac ccggccatca acattccgga cttcgaagct
     3361 atcgccaaaa tcgcacacga caacggcctt ccggtcatcg tcgacaacac ctttgctacg
     3421 ccgttcctct tccgcccgct cgaacacggt gctgacgtcg tcgtccattc ggctacgaaa
     3481 ttcatcggcg gccacggcac gaccctcggc ggcctcatcg ttgaaaacgg caaatttgac
     3541 tgggctggct cgggccgttt caaagaattc gcttcgcctg acgaatccta caatggtgtc
     3601 aactttgcca aggatatccc tggtgccggc ttcgtaaccc gtatccgcgc caaagccctc
     3661 cgcgacctcg gtgcctgcat cagcccgttc aacgcatggc tcctcatcca gggcctggaa
     3721 tccttgtccc tgcgcatcga acgccacgtc gctaacgccc agaaaatcgc cgacttcctg
     3781 gaaaaccatc cgaaagtcgc taaagtcaac tatccgtccc tcccgtcttc gccgtactac
     3841 aaactggctc agaaatactt ccccaaggga accggttcga tcttctcctt tgaattgaaa
     3901 gacggctatg cagctgcccg taaattcatc gacaatgcgg acatcttctc cgacctggct
     3961 aacgtcggcg actccaagtc cctcctcgtt cacccggctt cgacgacgca tcagcagctt
     4021 tcggaagaag ctcagcgcgc ctgcggtatc acaccgggca cgatccgtat ctctgttggc
     4081 ctcgaaaaca tcgaagacct cctcgccgct gttgaaaaag cactggaaac catctaagac
     4141 tattgtaagg agaaatgcac tatgcctatt aaaattgctg acggtctttc cgccaaggaa
     4201 aaacttcacg aagaaggtat cttcaccatc gataaggggc tggccctcca tcaggatata
     4261 cggccgctgc gcatccttat cctcaacctg atgcctttga agaaaccgac agagctgcag
     4321 cttctccgcc tcctcggtga ttcaccgctc caggtcgaag tggacttctg ccatacggac
     4381 agccatgaga gcacccacac cgacccgtct tatttagaag aaaactacta cgtcttcgat
     4441 gaaatcaagg ataactacta cgacggtttc atcatcaccg gcgccccggt cgaacagatt
     4501 cccttcgagt ctgtcgacta ctggccggaa atgaagactt actttgaatg ggccaagaca
     4561 catgccttct cgacgctcca cttctgctgg ggcgcccagg cagccctgta ttactactat
     4621 ggcatcgaaa agcgtctcct gccggccaag ctcttcggta tctttgaata ccagctgacc
     4681 cagaagcacc atcccctcct gcggggcttc gatgaccgtt acttcatccc ccagtcgcgg
     4741 catacggcga ttaacgacat ccaagtctac aatacgccgc agctcgacat cctctcacgg
     4801 tccgtcgaaa acggcatcaa catcatcggt acgccggatc accgccgttt cttcgtcctc
     4861 ggtcacttcg agtacgaccg gcggaccctg gaagaggaat acctgcggga taagaaaaag
     4921 ggcctgccca ttgccgtgcc caagaactac tatcctggtg acaacgatac caagcagccg
     4981 tacttcacgt ggtgcagcta cgctcacctg ttctatcaca actggctgaa catcgtctat
     5041 caggaaacac cttatgattt acaagccatc gccaaggtcc atccttgtgc gcattgaaaa
     5101 aaggcctgtc gcatttgcga caggcctttt ttacaccgta gggggcgcca cgtgggcgac
     5161 ccnnnnnnnn nnagccccta caataaacta caaacgatct ttattttcgc taaacgacct
     5221 acaatgaacg attacttcgt tccgaagatg cggtcgccgg catcgcctaa gccggggacg
     5281 atgtagcctt ggtcgttgag tttatcatcg agggcagcga cgtagatgcg gacgtctgga
     5341 tggtcggcgt tgactttctg gacgccctgg ggcgctgcga cgaggcacat gagcttgatg
     5401 ttctcgatgc ctttttcttt gagcagcgta atggcagccg atgcactgcc gccggtagcc
     5461 agcatggggt cgacgatgat gaaatcacgg tcttctacat cgggcagctt gcagtagtat
     5521 tcgaccggtt ccagcgtctg cgggtcacgg tagaggccga tgtggccgac tttggccgtc
     5581 gggatgaggt tgagcatgcc ctgtaccatg cccagaccgg cgcgcagaac cgggacgatg
     5641 cccagtttct tgccgctgat ctgtttgcct atcatcttgg caacaggcgt ttcgatttcg
     5701 acgtcttcca agggcaggtc ccgtgtgatt tcatagccca tgagcatggt gatttcttcc
     5761 aggaggttgc ggaaatcctt cgaacccgtt tcctttttcc gcatgagtgt gactttgtgc
     5821 tgaatgagcg gatgatccat tacttgtacg tgcatgtgtg aaatcccctt tcctatttat
     5881 ccatttctgc aatcttggcg acgcggacag cgtggcggcc gccatcaaaa ccagtggaga
     5941 ggaagatacg gacgatttct ctggccaggc ccgggccgat gacgcggccg cccatggtca
     6001 ggatgttggc gtcattgtgg cggcgggtca tctctgcttc caggcagtcg gcacagnnnn
     6061 nnnnnnacct gtgccgcaga cgaggatgcc ccggtcgaaa gtgccgtcag cgacggcttc
     6121 agcgactttc ttggcaatgt ccggataatc gcagcgttcg cccgtcaggg tgccgtaatc
     6181 ggtgaattca atgccttgtt ccttcaaata ttctttgatg ttttctttca ggtcaaatcc
     6241 gccgtgatcg gcgccgagta ctaatttcat aatcaattcc tcccatatat gctgataaat
     6301 tcagttttat tatacccctt tcggagtcat gtgaaaaggt ttaaccgaca atgaccacgt
     6361 ggccgccggc agcttttttc atgcggttca tgatagccag gcccaatccc tcatcgtcga
     6421 tgccttcggc caggatgata tcgacctggt cgctgtcaaa ggacaagagg cccgtgtaga
     6481 gctggttggc cagggcttcc ttgtcgccat ggcgacccca gatatacata tctttatgtg
     6541 ggaacgaggc ccctacttct tcgctgacca aaaagccgac ggttttgcct tcgcccttgg
     6601 cctggtcgta taaggcctgc agtttttccg tcaccttgtc gacagggccg acgacgacgg
     6661 tcatcggtgc cgatggcgca tagtggcggt atttcatgcc cggcgccttg gggacgccct
     6721 tgcccgtgac cagcgtcgta tcgagctgga cgtgaggtac gacttcctga agcatctcga
     6781 cagtgacgcc gccgggacgc aggatggtga cgccgtcgtc gccgttacat tcgacaatcg
     6841 tcgattcgac gccgatgtgg cagggaccgc cgtcgatgac catatcgacg cggccgtcca
     6901 tgtcgtgcat gacttcttcg gccgtcgtcg ggctgggccg ggtcgacagg ttggcgctgg
     6961 gaccggctac ggggacgccg gcacagcgca ggaaggcccg gcagatatcg tggtccgggc
     7021 agcgcagggc taccgtatca aggccgcccg tcgccgcatc aggcacgatg ggcttgcgcg
     7081 gcaggacgat ggtcagcggt cccggccaga agcggtccat gagcttctgt gccgtcggcg
     7141 ggatgacagc cgctgcctgg cgggcccctt cttcgtcggg cacggtcagg atgagcgggt
     7201 tatcgctggg ccggcctttg gcgacgtaaa tgctgcggca cgcgtcggcg tcgaggccat
     7261 tggcaccgat accgtagacc gtttccgtcg gaaagacgac caggccgccg tcgcggatga
     7321 tctggcccgc ttcttcgagg atggctttct gtttctctat gtccgtaatt tctacaagtt
     7381 tcgtctgttt catcgtctct actcttttct ggaaaatacg aggaccctgt cgatgccgcc
     7441 gtaatccttg acggtctgtt cgtggtcata ggcgccatgg cggcagaaaa cagccgtcac
     7501 ggcgtcggcc tggccggcgc cgatttcgac ggcacagtgg ccgccggctt tgacgtaagc
     7561 cgggctttct gcggcgatgc ggcgatagaa ttccagtccg tcgtcgccgc cgtacagggc
     7621 cagggccggg tcgtggcgca cttccggctg gagatgttcc atatcgccct tggtcaggta
     7681 cggcggattg gacaccagcc agtcacaagc gccttgcgga accttggtga ataaatcgct
     7741 ttcgatccag gtcacgcggc cgtctaggcc cagggtacgg ccattttcag cggctatggc
     7801 cagggctttg ggcgaaatgt cgacgccctt ccccgtggcc tgcggcagat agtggagcag
     7861 gctgaggatg atggccccgc tgcccgtacc gatgtcgatg atggccggcg ccgtcccttc
     7921 ggcagcggcg acgacagtct cgacgagcat ttccgtttcc ggcctgggaa tgaggacgtc
     7981 ttcactgacc ttgaaatcga ggcccatgaa ggccttggta cccaggatgt gggctacaga
     8041 caggcggtca gcccgctgtt tgaccatcgc ccggtaggcg gccagttctt caccgttcat
     8101 gggttcgtca taatgggcgt acaggtagat gcgctcttta tgcaagacgt gggccaggag
     8161 gatttccgca tcgaggcgcg gcgaatcgat gcctttgccg gcaaagtact gttgcgtcca
     8221 ttgcagcaag gcaccgatag tccaaatctt attcatcttc ggcttctttc atcaattccg
     8281 cccggcgggc ttcggccagg gcctggatga attcgtcgat atcgccgttg agaatgttat
     8341 ccagcttgta cagggtcagg ttgatgcgat ggtccgtcac gcggccctgc gggaagttat
     8401 acgtgcggat acgttcgctg cggtcgcccg taccgacctg gctgcgccgt tcttccgtca
     8461 attcggcatc ggccttctgc tgggcctggt cgaggaggcg ggctttcagg acgcgcaggg
     8521 ccttggcacg gttttccagc tgggaccgtt catcctgaca ttcgacgacc atcccagtcg
     8581 ggatatgggt catgcggacg gcagaactcg tcttattgac gtgctggccg ccggcaccgg
     8641 aggcgcggaa atagtcgacg cggacgtcgt ccatgttgag gtcgatttcg acgtcttccg
     8701 cttccggcag gacggcaacg gttgccgtcg acgtgtggat acggccgccg gcttcggtga
     8761 cgggcacgcg ctggacgcgg tggacgcccg attcatattt caacttgcca tagacgccag
     8821 cgccttcgac ggagaagacg acttccttga agccgcccag ttccggtgcg ttggaactga
     8881 tgatgctcgt cttccatccc ggctgttttt cgatgtattt catgtacatg cggaacaagt
     8941 cgccggcaaa gagggctgct tcgtcgccgc cggctccggc gcggatttcc atgatgacgt
     9001 tcttgtcatc ccgcgggtcc ttgggcagca tgaggacgtg cagctggtct tccagatcgg
     9061 cctgttcctt agtgaggccg ttcatttctt ccttggccat ggcgatgagg tcatcgtcgc
     9121 tgtcgggatc gtcgatgatt tccttgtcgc cgtcgatggt gctggtaatg tctttgtatt
     9181 tgcggaatgt ttcgacaatc ggcatcagcg aagcgtgttc cttggtcagt ttctgccatt
     9241 cgtcctgacg ggcgatgaca gacgggtcgc tgatctgctg ttccaggtcc atgaatttat
     9301 tctctacggc ctggacttta tccataaaag agctcataag gtcctcctca tttcaattcg
     9361 ttcgattcat tttcttcatc agccggcttg gcggcgttga gggcttcgat agccacttcg
     9421 atctggtcgg catggggttc ccgcgtcgtc agatactgga gccacaggcc cggacggaat
     9481 aaggcttgaa tgaccggatt ctgggtgcgg ccggcgaggc gtatcatttc ataactgatg
     9541 ccggcgacga cgggcatgag cagcacgcgg gatgcgatgc gttcgacgag attcggccag
     9601 cccaaaaagg caaagacgaa aatcgacacg accatgacga tgagcaggaa attcgtaccg
     9661 caccgcggat ggaggcggct gtggcgcttg acgttctcga cggtcagctc ttctcccgat
     9721 tcatacgtcc aaatggtctt gtgttcggca ccatggtatt caaagacgcg gcggatgtcc
     9781 gacgtcaggg aaatccccca tatatataat agaaatatag cgaggcgcag gaccccttcg
     9841 acgacgttca ggcgaaaggc actgtcagcc acgccgggaa tgaacttggc tgcatacgtc
     9901 gggatggcca ggaaaaagac gatggccagg acgacagaca cggccatagt cagggccatc
     9961 tgcgtgttcg acagctgctc atcttcttca ccggcagcct gggccgaata ggacaggctc
    10021 ttcatgccgt ataccaggga ctcgcacaat gcaacactgc cccgcaaaaa aggcttcttc
    10081 aaaatcggat accgttcggt aatggagtga acggcgtctt tctttacagt aatttcaccg
    10141 gacggcgtcc gcactgctgt ggcaatgctg acaggaccgc gcatcataac cccttcgatg
    10201 acggcctggc cgccgacgta cgtcttcggt ttcttcaaag agttgcctcc atttctgtac
    10261 atacaaatag agcagagcat ttctgctctg ctgcccactt gtactgctgc ttatttagct
    10321 gttttaccat aacgtttatt gaactgttcg atacggccgc gagcctgctg agcgcgctgc
    10381 tgccccgtaa agaacggatg gcatttggag caaatttcaa cacgcagttc cggtttcgta
    10441 gaacctgttt caaacgtatt gccgcatgcg caagtaactt tcgcattcgg gtagtacttc
    10501 ggatgaatac cttctttcac cacttacacc tctttctgta tcaatcattc aaaacctatg
    10561 ctagaatagt ttagcacatt ggcccttgaa atgcaaggat ttttttcccg caaaaggcga
    10621 taccgtattt ccatacacgc tgaattcccc gctggcggaa ttccgtcacg tattctcttt
    10681 cttcaatctg ctgcaacgcc tctttggcct tcgccgccat gtcagcttcc ctcgaagcca
    10741 ctttgaattc catgatgacg ccggcctaga cacatccttg ggaataattg ccaggtcgaa
    10801 gcggccgtac ccgctttccc ggttcgattc gataatataa tcggatacaa ataacgccgt
    10861 catgccaagg acgaagccgt gatagaaact ttctttattg gccgtatcat atacgctgac
    10921 caggtcccgg agatagcctc ccagcagttc cgaaaaggcc tttccgttgc ctgtaagnnn
    10981 nnnnnnnacg atatacatcc tgtacttccc ggttgggaat gaccaattca cagcgtatgc
    11041 cgccgattcc ccgcttgcgg ctgacagccg tcaaatagcc cgtcgtcagc agcatcgtat
    11101 agagggcatc cgtatcccgg ccaatatctt cataaatgac gccttcccgt atcaaagccg
    11161 tcacatggcg cccctgtaaa agagacagta aatccgtctc cgtttcagcc gtcacatggc
    11221 gcagcatttc ccggataatg ccatttcccg acgtattgac ccaataatct ccgacatcac
    11281 cgtgacggaa aaaattgaca atcgaccagg gattataaat ctccgcccca ccgaagagat
    11341 agccatcata ccacgctttc aaggccggaa gggccgtggt catggaaagg tcttctgcca
    11401 tctgggccac ttcctgtggc gtaaatccaa tgacatcccg ataggcagca tccatgacgg
    11461 agcaaacgtc gagattattc aaatcgctga aaatactctt tttggccacc cgcaggaccc
    11521 ccgtcagtac ggcaaaatcc aagtcgcgat tgcccttcag gacactgccc aggaactgtt
    11581 tcataaaagc gatgcactct gtataaaagc catgttccca agcctgctga atcggcgcat
    11641 cgtattcatc gagcaataaa atgacaggct tgccgaaatg ctgatgcatc atcttgacca
    11701 atagtgtcag ggccagctgc atttcattct gcccggcccc ctgctgtttc agtgccagga
    11761 agcgcctgcg taaatccggg ttcacagccg gactctctgc caaatactca tggtcgatga
    11821 accaataact aatccaggaa ctcagggatt gcaacatctg cggccaagta aggtccttca
    11881 aatctttcaa agacaagaac agcaccggat actggccgcg atgggccatg taggcagcgc
    11941 cggctccttc gatagccagt ccgtcgaaga gacggcggct ctgtgcctct ttttcgatag
    12001 aaaagaaatg ctccagcata ctcagggtca aggtcttacc gaaccgccgc ggccgcgtaa
    12061 tcagcgtcac cgcactgtgc ccgtcgatga gctggcggat aaaatccgtc ttgtcgacga
    12121 aataataatg cttactccgt atatcaaaaa aattatcgac accaatcggc atctgccact
    12181 tcataccatc accttctttc ataggtgggg ccttctcctg ttctttgtct tttattataa
    12241 caagaatcac gagaaacaaa aagaggagcc acgccatgaa gggtgagctg ccccccccca
    12301 atagttaggc caaaaatcta acgattgggg ggcagttttt ttatggcaaa acacagttat
    12361 gaatttaaga aaaaaatagt actgga
//
LOCUS       DAWABZ010000045        12310 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_1408_length_12310_cov_2.726071, whole genome shotgun sequence.
ACCESSION   DAWABZ010000045 DAWABZ010000000
VERSION     DAWABZ010000045.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 12310)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12310)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12310
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1408_length_12310_cov_2.726071"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4221..4230
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5354..5363
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7365..7374
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2705 a         3271 c         3522 g         2782 t
ORIGIN      
        1 tcgccaagtg ggctatctgc tgggcgtggc tgaactgggg cttgtccgaa aaaccggacc
       61 acttcttttc cggtaccagg tggaggtgct tgaggtactc catgggcttg cggcctgcga
      121 ccaggagcat cgatgcatcg cggggcttgt ccttgaggac cgacacgatg gccttgttga
      181 cattggaatt gaaagcaccg gccagccctt tgtccgaacc gatgatgatg tagcacgtct
      241 tcttgaccgt atcatgaggc gtgaacaagg cgttgcccgc atcgggcgaa gccgcagaca
      301 gacgctgcag catgccttcc atcttttcgg tatacggggc ggtagccacg gcccgatcct
      361 gtgcacggcg cagtcgtacg gaggaaacca tcttcatggc cttagtgatc tgctgggtat
      421 tggtgacgga tttaatacgt ctgtttattt cgcgtgcact cggcatagct tattcctcct
      481 ctttgatcag ttcgtgagaa gcgccgtact gcttggtgta ttcagcgata gccttctgca
      541 gcgtagcttc tgcgtctttg ccgagttcct tcgtcgattt gatgctttcg ccgacttccg
      601 gatagttact gtgcatgaac ttgatgaagc cgtcagcaaa ctgagaaact tcttcaacct
      661 gtacaggcat gaggtagttc ttgacggcca ggtaaatctg catgacctgg tcttcgacgg
      721 gcatcggctg atactgcggc tggatgagga gctgcatcgt ccgttcgccg gcgtcgatct
      781 gggctttcgt gctcttatcg aggtcggagc cgaactgggc gaaagcggcc aattcgcggt
      841 actgggccag gctcaggcgg agggtaccgg cgattttctt catggccttg atctgggcgg
      901 agccgccgac acgggaaacg gagaggccga cgttgatagc cgggcggatc ccggagttga
      961 aggcttctgt ttccaggaag atctggccgt ctgtgatgga aatgacgtta gtcgggatat
     1021 aggccgacaa gtcaccggcc agggtttcga tgatcggcag ggccgtaatc gagccgccgc
     1081 cgagttcatc ggacagcttg gcagcgcgtt cgaggagacg ggaatgtaag tagaatacgt
     1141 cgcccgggta tgcttcacgt cctggcggac gacggaggag cagactcatg gcgcggtaag
     1201 cctgagcgtg tttggacaag tcatcgtaga cgcagaggca gtccttgccc tggagcatga
     1261 agtattcggc catggaaacg ccggaatacg gggccaggta ctggagcggt gcgccgtcgg
     1321 aagccgtagc ggcgacgacg atggtatagg ccatagcgcc gtgttcttcc agggtccgga
     1381 caacgcgggc aacggtcgat gccttctggc cgatagcgac gtagacgcag atgacgcctg
     1441 tgtccttctg attcaggatg gtatcgatgg cgatggccgt cttgccggtg ccacggtcac
     1501 cgatgatgag ttcacgctgg ccacggccga taggaaccat ggagtcgata gccttgaggc
     1561 ctgtctggag cggtaccttg accggctggc ggtcggcgat gccgaaagcc ttgacttcga
     1621 tgaggcgtga atctttggtc ttgatttccc ctttgccatc aataggtatc cccaaggggt
     1681 tgacaacgcg gccgaccatg gcttcgccta cagggacacg catgacgcgg cctgtacggc
     1741 ggacggtgtc gccttccttg atgagggcgt catcgccgag cagtacggcg ccgacgttgt
     1801 cttcttccag gttcagggcc atgccgtaaa cgccgtgagg cagttccagc aattccccgg
     1861 acatggcttt gtccaaaccg taaatgtgtg caatgccgtc cccgacttcc agaacggtgc
     1921 cgacgtcgtc gacattcatt tccacattat aatcttcgat ttgtttcttg attatagccg
     1981 ttatttcttc tggcttcatt ttcattattc gtccgtcacc ccattcgtca cgtcagcctg
     2041 gagcagttta tgctgcatat cgcgcagctg ccgccggagg ctgccgtcta ttagtttgtc
     2101 gccgatctgg atgaccatgc cgccgatgag cgacgggtcg acgtagtaca gcggttcgat
     2161 gtgctgaccc gtcatggctt ccaggctggc aaccagtttc gtttcttctt tcttggacag
     2221 tggtttgacg acgcggatct tggccacttc gatgtgctgg gccttgcggg ccaaatcgat
     2281 gaacccgtca atggcaagca agagggccgc ttcccggcgc cggtcgatca tgacgtacat
     2341 gaactgcagg acgatcggcg ataggctgtc cttgaaaatc tttgcaagtg tgtctttttt
     2401 agactgcggc ggtacgaggg gatgattcag gaatttccac aaatcgggat tttcctggaa
     2461 cgtgtcccgt acgcctttca gttctgttcc gaactcggta agcttcttct gttcgctggc
     2521 gatgtcgaac atcgcctggc tgtatttgct atagatcgtt tcgggtatca taagatcagt
     2581 tcccctttac gcggtgcgtc tggagctggt cgatgcaatc ggctaccagt ttggcgttca
     2641 tgtcaggatc catgtttttc tggagcagtt tttctgccac agccatggac agtgtgacta
     2701 cttcattacg catttcccgg atagccgctt cccgttcgtt ggcgatttcc gtctgggcga
     2761 tctgtttttc ccgggcgatc tgggcgcgga tggcttccag ctgggcctgg gcttctttgt
     2821 cggcctgctt gacagctttg tcgacaatgg cctgggcttc agcacgggca tcgcggatct
     2881 gggcttcata gtcggctttc atcttttcag ccgtttcccg ggctttggct gcgttgtcga
     2941 ggtccgacgc aatcttgttg cggcggtctt ccaggacttt gagcagcggt ttataagcga
     3001 actttgccag aatggcaact aacacgaaga agttgatgaa ctggaacagc aaggtaccat
     3061 taatactaac caatgtactt ccctccccgg ctgtatatta gaatcttcgg attagagcag
     3121 gctgacgaac ggattggcga agaccaatac gatagcgatg acagctgcga taatcggcat
     3181 ggattcgatc aggccgatgg agataagcat gttgaccagg agtttgccgc ccatttccgg
     3241 ctggcgggcc gtaccttcaa gcatcttcga tgcggcatgg ccgtcaccga tagcagcgcc
     3301 gagagctgcc aaaccaatac ctaagcatgc accaaatgca gataatgcca atacgagagc
     3361 tttttccata ataaataacc tcctaagttg taacggtatc tgagcttttc ttagtcttcc
     3421 gtaaaggacg gtgccagata cgatgtagtc agaatggtga agatgaatgc ctggatgagg
     3481 ccgatgacga agctaaagag gatccaaatc atcgggatgg cgacaggcac gaggaagtac
     3541 aggacttcca acaggatttc accggccagg atgttgccaa agagacggaa ggccagtgtc
     3601 aacggtttgg tgatttcttc catcagattg atgacgacaa acggcacaaa gggttcgaag
     3661 aaatgcttga aatgcttttt gaacccctgg ttcttcaagg ccatgaagtg gaccataaag
     3721 gaagaaacca gtgccagggc gacagtcgta ttcaaatcat tcgtcggcga agccaggaca
     3781 tgcggattcg gcagcatgcc caattcgttg gcgaagagga tgaacaggaa catcgtcagc
     3841 aagaccgaaa cgacctggcc gtatttcggc cccaacgttt ccttgaactg atcgagcaat
     3901 gcttcgatga cgatttccat ggcgttctga agtcctgacg ggacgaggga ccggcccctt
     3961 gtcgcagcga cagtgacgat aatgaccaaa gccgcaacca gccaggtcat catgagcgta
     4021 tccaagttga cattcagacc caggagctgg acgaccatat gatgccctgc atgtaattcc
     4081 atacgcaata cctcctttcc tttaccgtct gagacgcgca gtcagtacat ctgcgaagac
     4141 gaggacgcgg tacgaaaaga taccggccac ggcagccccc agggaaatgg acgggaactg
     4201 caggatgacg atgacggcca nnnnnnnnnn gctgagcatg aagaaataaa cgatgttcaa
     4261 gccgctgccg atacaccagc cacttatgta ctgccaccag cctcctagaa aaaggacggc
     4321 cgtgacgata cacgtaaaac caacgagctt gtatattgtt gcctttacat aagcataaaa
     4381 cgcttccata ggctatcctc cgtgacattt cacttgcgca gatgccggcc atggcgcgcc
     4441 tcgtcctgct gataggacaa ggctttttta tataaggtcc agaatccgga gatggcaccg
     4501 atgaaggaaa aaagaatcaa tccccacggc gacgtcccgg catggacatc gatgaagtgc
     4561 cctatggcga cgccgacaca gatgcagacg aagagagaca aacctaagga accggcaatg
     4621 acaacaataa cccaaagcct catgtgattt aatttgtcat cgtgaggatc atcatgatgt
     4681 ctcacactac atacactcct gctcgggcga tggcagtttt ccataatcgt ttggtatagc
     4741 agttatatac tgccagcaac gattattggc ttattttagc acggaaaatc tggaaaatca
     4801 atacaaaatg aaagaaattt aaacgttata gctaaaaacg attctcagta tagcctaaga
     4861 caatacttag cggctaaaat catccggaac ggcttcttcc ccggcaaaaa cgtggagaat
     4921 agcctggacg atgcgctgcg aagccttgcc gtcgccatag ggattgacag cgttgctcat
     4981 ggcatcgtaa gcggcctggt cggtcaggag ccgcttcgtt tcggcataga cgacatcctt
     5041 gtccgtgccg atgaggcgta ccgtgccggc ttcgacggct tcaggccgtt cggtcgtatt
     5101 gcgcaggacc aggacaggct tgccgagaga cggcgcttct tcctggatgc cgccagagtc
     5161 ggtgaggacc aggctgctca ggctcatgag attggcaaag ggttcatatt ccatggggtc
     5221 gatgaggtgg atgcggtcga caccggccag ttcctcttcg acgactttgc gcaccagcgg
     5281 attacggtgg acaggaaaga cgatttccgt atcgggaatc tcttcgataa tgcggcgcag
     5341 ggccttatag atannnnnnn nnnagatatg gcgcatcggt tcacccagga tttcccggcg
     5401 gtgcgtcgtc aggaggatga cccggtggtt atggaaatcg acgtccttca ggtcgtcgcc
     5461 gaagtcatag tcgccggcaa cggtcatcat caaggcgtcg atgacggtat tccccgtcac
     5521 ataaatatga gaaggattta cattttcctt gagcagattg gccttggccg tcgccgtcgg
     5581 tgcgaagtgg atggctgcga tggcaccggt cagcttgcgg ttcatttctt ccgggaaggg
     5641 cgaatagatg tcccctgtcc gcagaccggc ttcgacgtgg cctaccggga cctgctggta
     5701 gtacgaggcc agggcgccgg caaaggtcgt cgtcgtgtcg ccatggacca ggaccaggtc
     5761 gggcttttcc ttggccagga catctttcag gcccatgagg gacttggtcg taatgtcgta
     5821 cagcgtctgc ccctgggaca tgatatccag gtcatagtcc ggtgtaatct ggaagagatc
     5881 cagtacctgg tcgagcatct gacggtgctg ggccgtaacg gcgacgatgg tctggatccg
     5941 gtcggcatgt ttctgcaatt ccagtacgac aggggccatc ttgatggctt ccggccgcgt
     6001 accgaataca gtcattacct tgatcataat aaaaaaacct ccttataaga caaccgattc
     6061 cacccactac tgtttgtggg tagaatcggt cgttgatttg cgcaatatgc caatgcgttt
     6121 tgccgaataa atgatgacgg caagaacgac caggacagtc acgatacccg tcttatagct
     6181 cacgttggct acaaagaggg ctacgatgcc gagaatcatg ctgacaaaat acattattaa
     6241 tacggcttgc ttctgtgtca tgcccagagc cagcaggcga tgatgcaggt gacccttatc
     6301 aggctggaaa atcggaacgc cgctcatctt gcggcggatg atagcaaacg tcgtatcgag
     6361 gatcggcaga cccagggcga tgatgggtac gacgagagcc acggtggctg ccgtcttgac
     6421 caggcctaag acggcggcga cggacagggt atagcccagg agcatactgc ccgtatcacc
     6481 catgaaaatc ttggccgggt tgaaattgta ctgcaagaag cccaaagccg ccccggccat
     6541 ggacacgatg accagagccg gcaggaactg gttcaggtta taagccagga ggaacatcga
     6601 aatcgatgcg atgaaggcga cgccggctgc caggccgtca agaccatcga tgaggttcac
     6661 cgtattggta aagccgatga tccagaaaat cgtcacggga atggcaatga tttctggcag
     6721 gacgatgata tcaccaaaag gattggtcag ccattcgatc tggatgccaa aggcgatggg
     6781 gatgcaggcg gccaggatct ggccgacgag cttgaccttg gccgggatat tgcagatgtc
     6841 gtcccagatg ccgacggcga cgatagccgt gcagcccagg agcatcccga ccagttcatg
     6901 ctgcaaaggc acgcagaaca agacggctgc catgaagccg atatagattg ccagcccgcc
     6961 gagccgcggg atgacatgag tatgtacctt tcttgcattg ggccggtcca cggcgccgat
     7021 cttgatagcg agtttcttga cgctcggcgt caggagataa gaaacgacta atgcaattac
     7081 aaagggtaca acatattgga gcacaacaca cgcctacttt ccattaatct atcagttcgt
     7141 cagactgcgc atgggcgccg ggatgcggcc gccgcgggca atgaattcat ctgacttgta
     7201 tttattgaca ttgatgatcg ggcaatggcc gaggaggccg ccgaattcaa cattgtcgcc
     7261 gacgtccttg cccggtacgg ggatgacgcg gacggccgtc gtcttgttgt tgatcatgcc
     7321 gatagccgct tcgtcggcga tgatggccga aatggtcgag gccgnnnnnn nnnnattgcc
     7381 ggggatggcg atcatgtcga ggccgaccga gcagacacag gtcatggctt ccagtttttc
     7441 caggctcagg ctgccgcggc cgacggcttc gagcatgccc ttgtcttcac tgacagggat
     7501 aaaggcgcca gacaggccgc cgacgcggga cgaagccacg aggccgccct tcttgacggc
     7561 gtcgttgagg agcgccaggg ctgccgtcgt gccgcaggca ccacagcttt ccaggcccat
     7621 ttcttccaaa atcgatgcga cgctgtcgcc gactgccggt gtcggagcca aggacaggtc
     7681 gatgatgcca aagggcatgt tgaggcgctt cgacgcttcc atggcgacga gctggccgac
     7741 gcgggtaatc ttgaacgccg tcttcttgat ggtttcggcg acgacgtcaa agggctggcc
     7801 tttgacctgt tccagggcga ctttgacgac gcccgggccg ctgacaccga cgctgatgca
     7861 cgaatcccca ttggtgacgc cgtggaaggc gccggccatg aaggggttgt cttcgacggc
     7921 attggagaag acgacgagtt tggcgcagcc taaggcatcc tggtccttcg tccgttcggc
     7981 caggtccttg aagacgcggc ccatttcggc gacggcatcc atgttgatgc cggccttcgt
     8041 cgagccgacg gcgacggaac tgcagacgag gtcggtcaca gccaggcttt cggcgatgga
     8101 gtcgatgagg atgcggtcgc ctttgctcat gccccgttcg accagggccg agaagccgcc
     8161 gatgaagttg atgccgattt ctttagctgc cttgtcgagg gtccgggcga tgtcgacgta
     8221 actcgtcagg tcgctggccc cggcgacgag gctgatgggc gtcacggaga cgcgcttatt
     8281 gatgatgggc acgccgtatt cgcgggagat ggcttctccc gtttcgacta agtgttcacc
     8341 gtatttcgtg attttatcgt aaatattgtt gcacaaggtc ttgccgtcgc tggcgcagca
     8401 gtcgaggagg ctgatgccca tggtaatggt gcggacgtca aggttgttct tttgaatcat
     8461 ctgattcgtt tcaagtacgt cttggattgt cagcatatca tttctcctta gcctacacga
     8521 tgcattgcgt caaaaatagc ctggttctgc gcccggattt caacgccgag ggcatcgccc
     8581 ttctgtttga gcaaatcctg taaggtatct aaggtaatat cggaattatc catgtccacg
     8641 atcatggcca tgttgaaaat gccatctaag atgacctggt tgatcgtcaa gatattgact
     8701 ttattttctg ccatgatctg gctgacggcg gcgatgatac cagtgcggtc cacgccaact
     8761 acggtgatta caactttcac agctgtccac tccttttcgt tattaaattc catgcttccc
     8821 tattatagca caggtagcgt actgaattcc actgtctgca tacgaaatga tagaattgac
     8881 gacaaacttg caactctgag ctatttattc aatgccttgc tgatgagggc cttgccttct
     8941 tccaggagct tgccggcctg gtcgggcgaa acgaagcttt cagcgtataa tttgtacagg
     9001 tcttcggtgc cggacgggcg ggcggcgacc cagccgtcgt cggtcgtgat cttgacgccg
     9061 ccgatggcga aatcaccata cggggacttg gagaggacgg ccgtaatggg gctgccgcac
     9121 aaggtctttt ccgtgacgtc ggcttcggtg agggcgctga tcttgtcttt ttcttccagc
     9181 gtcgccggtg catcgctgcg catggtgtac ggccggccca gtttttcaca gaggtcgttg
     9241 taatattcga tgggcgattt gcccgtgacg gcgcggattt cagcggccag gaggcacatg
     9301 atcatgccgt ccttgtcggt cgtccagacg ctgcggtcct tggcgatgaa cgagccgccg
     9361 gcactttctt cgccggaaaa ggcgatcttg ccatctgtat agagggggcc gaaatatttg
     9421 aagccgaccg ggacttcata gagcgggatg tccaggagct tggctgcccg ggcgagcata
     9481 tccgtcgtga cgacggtctt ggcgatgccc aggccttcga aattgcggtg cgtcatgaga
     9541 taatgggccg cgacggtcaa aaaggcgttg gccgaaatca tgccttccgc gccggagacg
     9601 atgccgaagc ggtcgtagtc cgggtcattg gccaggacga ggttatagtc tgcggcctgg
     9661 ccggtgacgg ccgacatgac ataggccgac gagcagtcca tgcggacctt gccgtcgtgg
     9721 tcgtaattca tgaaggtgaa ctgcgggtca tacgtatcgt tgacgaaatc caggtccagg
     9781 tggtacgtgt cggaaatctg gtgccagtag ttcatgcccg acccgcccag ggcgttgacc
     9841 aggatgcgca atttagcgtc gcggatggcg tccatgtcga tgatggagtc cagttcggcc
     9901 acgtagaggc ccttgtaatc ataagggacg acaaatgcgc tgtccttggc ctggtcatgg
     9961 gccatcatct ggacgccttc gttgtggccg gccaggagac ggttggcttc cctttcgata
    10021 gccttggtga tatccgaacc ggccggcccg ccggtaatgg ggttatactt gatgccgccg
    10081 tgttccggag gattgtggga cggcgtaatg atcaaaccgt cggccaggcg ttcaccacga
    10141 tggctgttgt agcgcaggat ggcccgcgat acagacggtg tcggtacgaa atcgccaaca
    10201 ctatcgacgt aagccgtgac gccgttggct gccaggacgg acaagaccgt ttcataagcc
    10261 agtttcgaca gataatgggt atcttcgccg acgaacagcg gcccttcggc gccgaactgc
    10321 ttgcggaagt tgcagacagc ctgggtaata gccgccacgt ggtcctgggt aaagctgccg
    10381 ttgccggcct ggccgcgatg gcctgatgtg ccgaaacgga cctgctgatg aggatccgcc
    10441 acatcgggat gcgtcgtatg atatgccgca gcgatggctt tcaaatcaat gtagtcttct
    10501 tttcttactt tggtacctgc caattcatgt actgccatac tatcacctgc ttcattattt
    10561 tgacaatcgc acagcgattt tatacaataa ggacgagtat gcggggttcc atcccccact
    10621 ccccatggct gtacgtcatc attgttatag tttacattca ttataacaaa aaatgtatca
    10681 atatcctaga tatataagga agaaaggtga tttgcatgac aaaatttatt tcctggaacg
    10741 tcaacggcct gcgggctgtc atgaagaaag gcttcatgga tacgttccaa gccctcgacg
    10801 ccgacgtctt ctgcctgcag gaaacaaagc tccaggccgg ccagatcgac ctggacctgc
    10861 cgggctatga acaatactgg aactatgccg accgcaaagg ctacagcggc acggccatct
    10921 tcacccgcct gtctcccctg tccgtccgct acggcatggg catcgacgag cacgaccacg
    10981 aaggacgcct gatcaccctg gaattcccgg aatactactt cgtgacctgc tatacgccca
    11041 attcccagag cgaactggcc cgcctgccgt accgcatgaa atgggaagac gacttccgcc
    11101 agtacctgct ggaactgcac cgggacaagc cggtcatcgt ctgcggcgac ctcaacgtgg
    11161 cccacgaaga aatcgacctc aagaacccca agacgaacca taagaacgcc ggcttctccg
    11221 acgaagaacg gcagaagatg acggacctcc tggcagcggg cttcaccgat acgtggcgcc
    11281 acttctaccc cgacgtgacg ggcatctatt cgtggtggag ctaccgtttc aaagcccgtc
    11341 agaacaatgc cggatggcgc atcgactact tcctgactac gaaggacctc gacgaccggc
    11401 tggaaaaagc cgccatatac accgacatca ccggcagcga ccactgcccg gtcggtctgg
    11461 aattgaaatg attttttcgc aaaggagcga tacgtatgtt aaagaaattg accacagcct
    11521 tactggcctg cacggctttt gccctgcccc aggccttcgc cttgtccgtc ctgcccgaag
    11581 ccggtgtcag cctcggcgtc ggcacgaaag aacagtacgc cgaagccaaa gtcctgccgc
    11641 ggacgaccat cggctataaa cgggccgacc gcgaccagta cggcaaccag aacgacatct
    11701 atgccaaata caatgtcatc ggctcgaaag tccagctcct cggcggctgg cgcaacaaca
    11761 tcgacgaaca gtcgagctcg acgtccttct acggcggcat cggcgtcgct gcgccgcaca
    11821 tcctgggcct ccagccctat gcgacctacg tcaagggctc gcactttgcc gaagcccagg
    11881 ccggcctcaa ttacaacatc gccttcggct ggggcctgaa cgtcaattac cataactaca
    11941 tgcccgaaga cggcgacaac gaacacggcg tcggcgccgg cgtgaccttc catttttaaa
    12001 ggacgcagtt cgcaggccgc agttcgcatt cgcaggccgc atcgtaagtg ccgtcccagg
    12061 ggcggcaaaa ggcaacctct ccggccctat gggccacctc tcctatcagg agaggcttaa
    12121 ggctcccttt ataggggagc tggcagcgta gctgactgag gggttgcttg tcattataaa
    12181 aaagggcttg tcgcacgaag gcttttgcca tcatgtgata tccctttctc tttatagttt
    12241 gtagtgtgga gtttgtagtt tgtagaagct gattttaaat tttttaccat ttgctgcaaa
    12301 ctaaaaacta
//
LOCUS       DAWABZ010000046        11659 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_1501_length_11659_cov_3.477163, whole genome shotgun sequence.
ACCESSION   DAWABZ010000046 DAWABZ010000000
VERSION     DAWABZ010000046.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 11659)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11659)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11659
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1501_length_11659_cov_3.477163"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4067..4076
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10415..10424
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2714 a         3186 c         3200 g         2539 t
ORIGIN      
        1 aactacaaac ttaaaactca aaaagaggct gtcttaatgc gacagcctct ttttgcatca
       61 gtcagtagga taaaagcgga tgacaggctt gaagtatatt aataatgtac ttctgggtac
      121 tggagttaca gaattaaaac tatacttttt ttgtctttta ttgtataata ctaaatatag
      181 acaacaaggg ggtagaaata tgagattgaa tcaggctacg gactatgcct tccgcatggt
      241 cctctatttg gcgtctctcc cggaaggcac gaagattacc ggcgcggcgc tggctgaaaa
      301 acagaatata ccggaacgtt ttttattgaa aatcatgcgc agtctcacca aggctggcat
      361 catgaagtct taccgcggtg tcgaaggcgg cttcgccctg cagcgggctc ccaaggatat
      421 caccctcttt gacatcatcg atgccgtcga agggcagacg gaactgcagc gctgtctcca
      481 tgatatcggc tcgtgcagcc gcggctgcag cggcatgtgc tctatttatg ccgcttttgc
      541 cgacatccag cgcgacctgg cggcccacct gaagtccatc aacttcgaga cactggccaa
      601 acaggaagcc aatctgcgca tggtccatga agcagaagcg cagctgacgg ccaaaaaaga
      661 agcggcagaa aagtgagctt gtagtacgga gtttgtagaa gagggcttta aatttaaaac
      721 cctccgctaa ccactagcca ctaaaaaggg cttgtcaccg acgacaagcc ctttttttta
      781 tgcccaaaat attgtaaaat aaggataccc attttatttt tattctcgtt gtgtggagtg
      841 aagccctatg tacctacccc ttcaaggttt catcagcagc ttccggctgc tcgatatatt
      901 ggatatcctg ctcgtcgctt ttgtcctcta taaattattc atcctcatcc gcaatacccg
      961 ggccgtggcc ctgatcaagg gcctgctggt cctgggcgtg gtcactgtcg tcagcaaggt
     1021 cctcgacctg cacgtcatca actggatgct gcagcagggg atgacggtca ttctcgtcgc
     1081 cctgcctgtc gtcttccagc cggaattgcg gcgggccctg gaacaaatcg gccggggccg
     1141 tttctttcgt tccggccagg tcatcagcat ggaagaactg gaacggctca tcgatgaaat
     1201 cgtggctatc agtgaatccc tgtcaaagac ccatacagga gcgctcatcg tctttgaacg
     1261 cgaagtaggc cttaacgact acatcgatac aggcatcgcc atcgacggcc tggtgtcgcg
     1321 ggaactgttg gggaatatct tcattcccaa tacgcccctt cacgatggcg ccgtcatcat
     1381 ccgggaaaac cggatcatgg ctgccggctg tctgctgccg ttaacgtcgg accggtcgct
     1441 gtcgacggaa ttagggacgc gtcatcgggc ggctatcggc atcagtgaac tggccgatgc
     1501 cgtcgtcgtc gtcgtcagcg aagaaacggg ctccatttct tatacctatg gcggtcatat
     1561 ttatcgtcac ctcgacggca atgccctgcg caatgtcctg aagacgttct tgaacaggac
     1621 gcctcagggc ctggccaata tttttaaatg gaggccttag cttatgaaca agattgataa
     1681 aaaatggcag ctcaaaataa tttgcctcct cttggctatc gtcttgtggt tcgtcatcat
     1741 caacgaacag aatcccctca gtgaaggcag ttataccgta ccgatcactg tagagaacct
     1801 cgactcccag tatattacgt ccaacgtgcc caagacggtc tatgtccgcc tgtcgggacc
     1861 gcgcaatacg atcatcaacg tcgggccgtc ggatatcaag gcctatatcg acttgtccag
     1921 tgtccaggaa ggggaagtag aggtcccggt ccacatcgag atccccagcg ggacggagct
     1981 gaagaagcag agcatgacgt cgacgaaaat cacagtcgat gtctatacag tcaaagaatt
     2041 taaattgacg ccccatgtcg tcggcaaact ggatgacaag gatttcatca gcgatttgaa
     2101 aatcgtaccg gagaaagtcg tcgtcagcgg ggcgcgccgc ctcatccagc acgtcagcca
     2161 ggcagtcgta gatatcccgg tcaatcagcg caatggcgac tttgctatta tggcgccgat
     2221 tcatttgtat caggctgatg gcacgcctgt cgaaggactg gaaatgacgc cctggcagag
     2281 caatgtcaaa gtggccattg cccatgatgc cgtggccaag acggtgcctc tcaacctgaa
     2341 cgctacgggc cagacggcct ggaagaccgt cacagtccag ccgatgtcgg tccaggtccg
     2401 cggtgaagcc gatacgctga agaatatcag ctccatcgat ttgccagata tcgatgtgga
     2461 aaacatgacg gaagaaaaga cctggaaagt gctgattccg cccattgatg gtgtcgtcat
     2521 agaccctgat gaagtcgacg ccacactgca tattaagaaa gatgagtaga aagggagaag
     2581 attgtgctta gagtattgaa tatccgcacg gctgtgccca tgaagaagcc tttggaagcg
     2641 ttagttgcca agaagctccg ctgcagccgc agcgatattt cccgccttat cattgtccgc
     2701 cggtccatcg acgcccggcg gaaaccgcgg atttatttcg tttttacggt cgatgtggaa
     2761 ttcgtcgacg aaagccgcgt ttggaagcgc tgtcgtgaca ataaggatgt ccgccagctg
     2821 gctgagtcgc cggcgccgga tatccggccc ggtacgcagc cgcttaccca ccggcccatc
     2881 gtcatcggga ccggcccgtc tggcctggcg gcagccctgg ccctggccga acacggctat
     2941 gcgcctatcg tctttgaacg ggggtgtgac gtcgataccc gcacgaaaga cgttgaagat
     3001 ttttggcgcg gcggcccctt caaggcatcg tcgaacgtcc agttcggcga aggcggagcg
     3061 gggacctttt ccgatgggaa attgacgacc cgcgtcaatc atccgctgct gcggcgcatt
     3121 ttgcagactt tcgtcgatgc cggagcgccg gaagacattc tgtatgccta taatccccac
     3181 gtcgggaccg atgtcctgcg ccgcgtcgtc aagaatatgc gccacaccat cgaggcgcag
     3241 ggcggcgaag tccatttttc cagctgtgtc acggcaatcc atcgcgacga tagcggacgc
     3301 atcacgtcgg tgaccgtcaa cggctgcgat gagtacccgg cagagctggt cgtcatgggt
     3361 atcggccaca gtgcccgcga tacgtattac atgctccatg accagggcat taccctggaa
     3421 aagaagcctt ttgccgtcgg tgtgcgtatc gaacattccc aggatgtcat cgaccgggcc
     3481 caatatggct gcccggcagc cgacctcggc ctggaaccgg ccgattacgc cctggtctat
     3541 cacagcccgg aagggcggtc gtgctattct ttctgcatgt gtcccggcgg tgtcgtcgtc
     3601 ggcgcggctt cggaagaagg gcgggtcgtc accaatggca tgagcctgta ccgacgtgac
     3661 agcggcatcg ccaacagtgc tgtcgtcgtc aacgtcacag ccgacgacat gggcggcgac
     3721 agccccctgg cggggatcga attccagcgc cgctatgaag ccctggccta tcaggccggc
     3781 ggtagcaatt atcacgcgcc ggctcagacc gtcggggctt tcctcagccg tcctggagca
     3841 ccgcaggaag ggcccatcca ttcctatcgt cccggcgtga cctggacgga cctgcatgag
     3901 gtcctgccag gtttcgttac gacgacactg gaacaggccc tgccttattt tgggcggcgg
     3961 attcacggtt ttgacgatga cgatgtcgtc atgaccggcg tcgaaacccg gaccagcgcc
     4021 ccggtacgca ttgtccgcgg cgaagaccgc atggccctgg gggcggnnnn nnnnnnggca
     4081 ggcctttatc ccatcgggga aggggctggc tatgccggcg gcattatgag cgcttttttg
     4141 gatggcctgg aaacggcgat tgaaatcatc cgtaaatatc aacctttgga ggtaaagtaa
     4201 atggctagat tgtttggaac tgatggagta cgaggtattg ccaatgattc gctgacaccg
     4261 gaattggcat atcatttggg cagagcagct gcctatattt ttgggcagaa caaagaacac
     4321 ccgacctttc tcatcggccg ggatacccgc cgttccggca ctatgctggc agatatcctg
     4381 gccgccggta tcgcttcggc tggtggtgac tgctacatgg taggcgtcat tccgactccg
     4441 gccattgcct acttgacgcg gactcatcat atggatgccg gcgtcatgat ttcggcgtcg
     4501 cataatccct ttgaatacaa cggtatcaag ttcttcgacg gctttggcta taagctgcct
     4561 gacgaaacgg aagacaaaat cgaagaatgt atgctcaaag atgaacgggc tgcccttcag
     4621 agccgtccga ccggggccgc tatcggcacg atttcgacgt ggaccgattt acagcaggaa
     4681 tatgccgatt tcatctgcaa atcgacggat gtcgatttgt cgggcctgaa agtcgtccat
     4741 gacggggcca atggttccgc ttcggtcttg gggccggaaa tcctcaaacg cctgggtgcc
     4801 gaagtcattg ccatccatca tgaaccgaac ggcatcaaca tcaatgacaa ctgcggctct
     4861 acccatttgg aaagcttaaa ggaaacggtt ctcaagtatg gggccgacgt agggattgcc
     4921 aacgacggcg atgccgaccg ctgcctggct gtcgatgaaa agggccagga aatggatggc
     4981 gaccagatca tgctcctctg cgccatgaaa ttgaaggccg aaggccggct caaacagaat
     5041 accgtcgtcg gtacggtcat gagcaatatc ggtttccaca aagccctgaa ggaaatgggc
     5101 tgccagacgg aaattacggc tgtcggcgac cgctatgtcc tggaaaagat gcgcaaagac
     5161 ggctattccc tcggcggcga acagtcgggc catatcatct tcctcgacta caatacgact
     5221 ggtgacggcc tgctcacggc ggtccagctg ctgagcatca tgaaacagca gcagaagccc
     5281 ttgtcggaac tggccggatt gatgacgcgc tatccgcaga ttttgaaaaa tgttcgcgtt
     5341 cagacgaaat cgggatggga agacaataac ctcatcatgg ccgccatctc ggctggcgaa
     5401 gaagagttgg gcgatgaagg ccgcatcctc gtccgcccgt cgggcacaga acccttgatc
     5461 cgtgtcatgg cagaaggacc ggacctggaa cagctcgaac gcatcgtcga tgacatcgcc
     5521 gtcgtcgtcg aacacgaaat gggcccgtct aagtaggtat tgacaaaacc ttgcaaatat
     5581 attaaagtgt gggaatggag ttcctggaac tctttcctgc acttttttat gcaggcgtct
     5641 ggaaatcctt gacgatataa tggatagcgc tagtgcctga tggattcagg cagacgaggt
     5701 agagaagtat cgaaatagtc agcggatgtc tctcggcagc ttcagccgtt atatggcgga
     5761 aaaacacgat ggtaacgtcg tcgacaagtt cgtcataaga aggaacattt tcatatcagg
     5821 aggatataac tatgtgtggt attgtaggtt acatcggaga cagagaagca gcagattttt
     5881 tgatggatgg actggccaag ctggaatatc gtggttatga ctcggccggt atcgccgtct
     5941 acaacgacgg cgccatcgct gtccgcaaaa aagcaggccg tctggtcaat ctggctgaag
     6001 aagtcaaggc tcaccccgtc gtagggacgc tgggcatcgg tcatacccgc tgggcgacac
     6061 acggcggccc gtcggacgtc aatgcccatc cccatacgga tgaaaagggg aacttcgcca
     6121 tcgtccacaa cggtatcatc gaaaactaca tggaattgaa ggaagaactc ctcaaaaaag
     6181 ggtatcactt caagtcggaa acggatacgg aagtcgtcgc gcacctctgc gccgacatgt
     6241 acgacggaga tttcaccagc accgtccgca aggtcctggc ccgtctgcgc ggttcctatt
     6301 cgttggtctt catgtgcaag tttgaaccgg ataagatcat ttgcagcaag aaagacaatc
     6361 ccctcatcgt cggcctcggc cagggggaaa attacatcgc ttcggatatt ccggccatcc
     6421 tcagctacac ccgcaagacg tacatcatga acgatgggga aatcgccatc gtcatgaagg
     6481 ataatgtctg ggtcacggac ctcgaaggca agcccatttc caagaaggtc tttgaagtca
     6541 actgggatgc tgaagcagct gaaaagggcg gctttgaaca cttcatgctc aaggaaatct
     6601 acgaacagcc gaaggctatc aaagatacca tgagctgccg catcaccaaa gatggcaagc
     6661 acgtccattt tgaggaattg aactggacgc cggaagacgt caaatcaatc ggcaagattt
     6721 tgattactgc ctgcggtacg gcctaccatg ccggcctttt ggccaagtat ttcattgaaa
     6781 aactggcccg catccccgtc gaagtcgaca tcgcttcgga ataccgttac cgcgatccct
     6841 tgacggacag caagaccctg tgcattgtca tcagtcagtc cggggaaacc atagacaccc
     6901 tggcagccct gaaagaagcc aaacgccgtg gcgctcgttc cctggccgtc accaacgtcg
     6961 tcggttcgtc tatttcccgt gaagccgacc aggtcgctta tacctgggct ggcccggaaa
     7021 tcgccgtcgc ttcgaccaag gcctatacga cgcagctggt cacgctcctg ctcctgtccc
     7081 tgtacatggg ccgcttgaac gggaacctgc ccgtagaccg ggaagaaaaa atcctggctg
     7141 atttgaccaa actgccggat cagtgccagg ccatcttcga ccaggtcgat ttcatcaaag
     7201 gtctctgcag tactttcaaa gataaagaag acgtcttttt catcggccgt tccatggact
     7261 acggcctggc tatggaaggt gccctgaaat tgaaggaaat ctcctatatt catgctgaat
     7321 cctatgccgg cggcgaactg aaacacggca cgttgtccct catcgaagcc ggtgtccccg
     7381 tcatcgccct ggctacccag ctggaagtcg aagaaaagat gatcagcaac atcaaggaag
     7441 tcaaagcccg cgatgcctat gtcgtcggcg tcgtccagga tggcgatacg gaagtcggca
     7501 agaccgtcga tgatttgatc aaagtaccgg catcggacga tttcgtcgtt cctatcctgg
     7561 ccatcatccc cttgcagctc ctggcctatt acatcgctgt cagccgcggc aacgacgtag
     7621 ataaaccgcg taacctggct aagagcgtta ccgtagaata agagatagtt tgtagttcag
     7681 cgtttgcagt ttgtagacga cggttttaaa tttttaaaca ttcactacaa actaaaaact
     7741 acaaacttcg aactaaaaaa gaggctgtcc cagcgacagc ctcttttttg tcccatttat
     7801 tctccgttcg gcagaataat atctcctttc gtcatggaaa agaggcggcc gtcttcgttt
     7861 tgccgtatac tatacttaac gaaatgacag tgatgattgt agatagatgt gaggtgttgt
     7921 cttatgtcac gatttataaa taacctgaaa cgccgcgttg ctaaagaaca gaagaaaatc
     7981 gtcctgccgg aatcggaaag ccgccgcgtc ctgcaggctg ccgaacgggt ccaggccgaa
     8041 ggctttgccc ggcccatcct catcggccgt ccccggagta tcgtcgaagt cgcttcggaa
     8101 taccagatcg atatggacgg gattgaaatc atcgaccctg aaacgtaccc catgatggat
     8161 aagttctgcc aatattatgc caaacggcgg gctaagaagg gcatgagcct ggaagaagcc
     8221 cggaaagtcc tgtcagaaaa ttacatcttc tttgcagcct gcctggttgc cttcgatatt
     8281 gccgacggca tggtcgccgg tgcggtggcc acgtcgtcgg aagtcatccg ggcggctctg
     8341 caggtcatcg gtcctcatcc cggcctgtcg acggtcagca gttctttcat catgattacc
     8401 gataagcccc agttcggcga tgacggtatc ttcgtcgtcg gcgactgcag tgtcgtcatc
     8461 gagccgacgc cgcagcagct ggccgatatt gctgtcagct gtgtcgaacg ggcccgccgg
     8521 acggcgcaga tgctcgatcc gaaagtagca ttcctatcgt attcgaccat gggcagcggt
     8581 gctggcgaag aagtcgaccg cgtccgcgag gccgtccgcc tgctgcggga ccgcaatgtc
     8641 gattttgaat tcgacggcga aatgcaggcc gatgccgcgc tggtaccgcg cattgcccgc
     8701 cagaaagcac cgggttcggc cgtagccggt caggccaatg tcctcatttt cccgaacctc
     8761 gtttccggca acatctgcta taaagtattg gaacacctgg ccggggctac ggccttggga
     8821 ccgctcctcc agggcctggc caagccggtc atggatttgt cccgcggctg tacgcctgaa
     8881 gatattaccg acgtcatcgc catttgctgc agcgatgcca tctacatgca ggctgaaaag
     8941 aaacgcgata tcgcctttac cagccgtttt gaaaagctcg accgccgcgt agccgtcgag
     9001 aaccgcaata tttccatcca gttcgacccg gaaaaatgca agaactgtac tctctgccgc
     9061 cggcgctgtg ccgatgtcat gtccatgacc ggttattatt ccctggaaag taccggcgac
     9121 gtgcctatct gcgtccactg cggccagtgt tccctgacct gcatgtttgg ggcgacgacg
     9181 acggtatccc agcgcgatgc tatccagcag gccatcgacg atcctaagaa agtcgtcatc
     9241 ttccagacag cgccggctgt ccgcgtcgcc ctcggcgatg aattcggact gcctttcggt
     9301 tcggttgtcc agggcaagat gatcgccgcc ctgcgggccc tcggcgggga ttatgtcttc
     9361 gatacgaatt tcggcgccga tttgaccatc atggaagaag catcggaact cttgtaccgc
     9421 atgcataaca agaaagaact cctgccgcag ttcaccagct gttgtccgtc gtgggtcgaa
     9481 ttcacggaaa tcttcttccc tgaactgatt ccccatctgt cgacggctaa gagccctatc
     9541 agtatgctca gtcctatgat caagacgtat tttgccaaac gcatgaagat cgacccggcc
     9601 gatatcgtga cggtctgcgt gacgccgtgt acgtcgaaga aggcggaaat cgcccggccg
     9661 gaacggaatg ctgccggccg ttactggaag aaaccggaaa tccgcgatac ggactactgc
     9721 attacgacgc gggaattggc gaaatggatc aaagacgccc aaatcgactt caacagcctg
     9781 aaagattcgg attacgaccc catcttcggc cagtcctccg gcggcggcac gattttcgga
     9841 aacagcggcg gcgtcatgga agcggccctg cggacgttgg tctatttgaa gacgggcaaa
     9901 ccggctgatg aagaattcct ccactttgaa gaagtccgcg gcttggaagg cgtcaaggaa
     9961 gcggccctga ccctggacaa cgacatcatc cgcgtcgtcg ccatcagcgg tctggccaat
    10021 gcccggcgct tcatcaacct catggaagaa aaacattcgt ggaagaaata tgccttcatc
    10081 gaagtcatgg cctgcccagg cggctgcatc ggcggcggtg gccagccgag aacgaaactg
    10141 ccacaggaaa tcccggccaa acgcgcccgc atcgaatcgc tttacggact ggatgcactg
    10201 aaccagatac gtgccagctg ggaaaacccg gaaatccaga cactgtatac caagttcctg
    10261 ggtgaaccgc tcagcgacct gtcggaatcc ttactgcaca cgcagtatat caacaagcac
    10321 tacatgctgg gcaaagaaga taaagtccag ccaggcgtga gccattaacg tgattagtgg
    10381 ttcgtggcta gtggctagtg gcttcgtagg ggccnnnnnn nnnncctctc ctgttaggag
    10441 aggtggcccg aagggccgga gaggttaacc ctctcagtca gctgtagctg actgctcccc
    10501 tataatggga gccattgtac gaactgctaa cagctagctg ctgactgcta aaaaggcgct
    10561 gtcttcatgc gacagcgcct tttcacttgc ttcctttccc gtatgtcctt ataatgaaag
    10621 gtaacataca tgatgcggaa gggaatcgat atgagccgat atattacgtc tttgaaacag
    10681 caggcggccc aaaagccgca aagaatagtt ttgcctgaaa gtgatgatat ccgtatattg
    10741 caggctgccg accgggtatt ggccgagcag ctggccatcc ccgtcctttt ggggaaggaa
    10801 ggcgacatac aggaactggc ttcacggtat cagctcgatt tggaaggggc tgaagtgatt
    10861 tatcccaaga agtaccctgg tttagaaagc ctggcccaga cctatgcgga taagcggaaa
    10921 gccactggta tgaccttgga agagagccgc cgttttctct tgaatcatcc cttgtatttt
    10981 ggggccgcct tagtcgctgc cggggaagcg gccggcatgg tagccggtat cgaaacgccg
    11041 gcgccggaag tgctccgggc ggctttgcag ctcattggga cgcgggatga catcgatacg
    11101 gtcagcagtt cttttatcgt cgtgacggat atgatgcagt tcggtcatga cggcgtcttc
    11161 gtcgtcggcg atggcgatgt cattcccgac ccggacgaat accagttggc cgatattgcc
    11221 tgcagctgcg tcgaacgggc gcggcggacc ctgcacattg cctcgcccaa agtggccttg
    11281 ctgtcgtatt cgaccatggg cagtgaccgc ggtgccggtg ccgataaagt ccgcaaagcc
    11341 gtgcagatcc tgagtgaccg caatgtcgat ttcatctacg atggagaaat ggaggccgac
    11401 gtggccctgg tcccagcgta tgctgccgat tgggcgcccc gttcgcccgt ggcagggcag
    11461 gccgatgtcc tcgtctttcc cgacctcaat acgggcaaca tctgctgcaa gatgctggaa
    11521 cacgtcgccg gggcgaccgt cctggggccg ctgctgcagg gcctggccaa gccggtcatg
    11581 gacctgtccc gggcgtccac tgtcgatacc atcgtcgaca ccatcgtcat ctgctgctgc
    11641 gatgcgtgag aatacgcag
//
LOCUS       DAWABZ010000047        11599 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_1513_length_11599_cov_3.442654, whole genome shotgun sequence.
ACCESSION   DAWABZ010000047 DAWABZ010000000
VERSION     DAWABZ010000047.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 11599)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11599)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11599
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1513_length_11599_cov_3.442654"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    669..678
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2647 a         3028 c         3227 g         2687 t
ORIGIN      
        1 ttcggtttgt actagagctt tttctacggc ctttacgggg ggcaattcac tctttttttt
       61 ttcgtatccc gaaagttccg catcaccttc caaaaagccc tgcatgtggt acgtgccgcc
      121 cagtatctga gataaagcgg catcggtgaa tttgaagtaa tcttcccgat tggcccgttt
      181 caccatgaag gccgtcttaa aggcgacgat gacctggcct tcgccgatgt aaacgacgcg
      241 gccattgcgg atacaggaca agacggcttt cttcttttcc ttgtcgagaa tggcacacat
      301 ctttttccag acgtcctggt aggctgccga ctcgacgggg ggccgtgctg cctgaacagg
      361 cggcgttgcc ggcgctttgt tgtcggccgg agccgcggtc tttacgacag gtgccgccgg
      421 ggctggaact gcgggctcaa cggggcgcac cggctgaggg actggcgctg ccgcttcccg
      481 ggctgccgga cggacggccg gagccggttc gggaggggta aactcatctt ccgggaaggg
      541 gaccgcttct tccggaccat aagccccgct gtaatcgtct tcttccggta cggggatgac
      601 gtccggcttt gtggcagccg ccgtcttggc ttttctcccc gaagcctggg gagccacagc
      661 cgatatacnn nnnnnnnnac tgacggcagg cggctgggtc agctgccgcg ctgccatgag
      721 caggcccatt tccacggcaa tgcgcggaac cggcgacgtc ttggccgtca ataaggcctg
      781 ctgcaaaatc gccaggacct gaaaaacact atctgccgtc cattcgccgg cctgcctgcg
      841 aagttcatcg aggcagtcat cataagctga aagagtatcc aacgaaggcg cagcctgaca
      901 gagcatgaca gcccgcagat gggccagcaa ttccgtcagg atgacccgcg gctccttgcc
      961 catgtggatg acgtcatcga cagccttgat gatgacatcg cctttcccat cgaaaatagc
     1021 ctgggtcagg gaaaacagcc agtctttgcc gatgaggccg agtaaatcgc ggacgacgcc
     1081 ttcgtcgata gtgccgctcg tgttgctgac gcactggtcg agcatactca gggcatcgcg
     1141 catgccgccg tcggcctgga cggccaggat atgagccgca cccttggtca ggtcgatctg
     1201 ttcctgaccg gccacgtata gcaggcgctt ggcaatatcg tcgctggtaa tgcgccggaa
     1261 ctcataacgc tgacaccgcg acaggatggt catgggaatc cgttccggtt cggtcgtcgc
     1321 caggatgaag atgacatggg ccggcggttc ttccaaggtc ttcagcaggg cattgaaagc
     1381 ttcggtcgtc agcatgtgca cttcatcgat gatataaatt ttcttatgcc cttcggcagg
     1441 catgaattta atcgtttccc gcagggtccg gatatcctcg atgctgcggt tggaagcggc
     1501 gtcgatttcg acgacatcca gggattcgcc gctcatgatg tggcggcaga cttcacattc
     1561 gttgcacggg tgggacgtcg gcccgtggac acagttcatg gcccgggcaa aaattttagc
     1621 cgtgctcgtc ttgcccgtcc cgcgggggcc ggagaataag taagcatggg ctactttgtc
     1681 cgtatctatc gctttttgca gggtttccgt aatgtgggac tggccgacga catcttcaaa
     1741 attcctgggc ctccacttac ggtataacgc aatatatgcc atgagtcctt ttccttccgt
     1801 atacgaaaaa aagcagctaa ccgctgcttt taccaactga aaagtactcg tgtatgataa
     1861 cccgccaggt ttcctcgtgg cacatggaaa tgtctactta ttgctgcttc ctctcggacc
     1921 tgacaaggtt catagattcc cattgcacaa gccccggcgg attaccacgc accagtaatc
     1981 ttattaagtt agctatatta ttatatcgtg aaaaacgaag tttggcaagg aaaatttccc
     2041 tttttgagag aatttagtgg ttaggggcta ggagctaggg gggtagtggt tagtagttag
     2101 tggttagtag ttagtggttc gtggttagtg gcttgtaggg ggctccacgt gcgaacccct
     2161 acagaaaaaa aggacccctc gcacgaaagc ttctaccatc atgcgagggg cttttttggt
     2221 caaaaacaac ctctcagacc gccttcggcg gccagctccc ctataagggg agccgctagc
     2281 cacgaacaac tagccacgag ccactatctt agtgattctg ccagatatct acgcggcggt
     2341 tgtcagcgcg gccctggtcg gtgtcgttgg tgctgacggg atctgtcttg cctttgtagt
     2401 cgagctgcat ctgtgttgcc gggatgccct ggctttcagc ataggctgca acgttgtcta
     2461 cgcggcgttt cgagaggtca tcgttgtaag catcgctgcc tttagcatcg gtgttgccga
     2521 cgagtttgaa cgtatcgttc gtgtttgctt tagcaacctg gacgaagtgg tccatcttag
     2581 cgcgttcgga aggaagcggc tggtcttcgt cgaagccgaa gtgaacgctt tcaacgtagt
     2641 agtcgttctt ctggaccggc gtttcaacga ccggtgccgg agtcggttcc ggcgtgtaaa
     2701 ccggttccgg agccggagct tctttgtggc cgccgccgaa gcgataggag aggccagcga
     2761 tgaagccttt gtaggagatg ttagcgtcgt ctttcaatgc accgagggtg ttgtcgcctt
     2821 catcagccaa tttggtgttc aagtaacgat agccagcgct gatgtcgagg tccggtgtga
     2881 tgctgtagcc gaggccagct tcccacatgg tcgtcttctt ggtaccgaca gcgcctttag
     2941 cgtagaagtc gaggttctta gccagcggag ctttggcgat caagccgatc tgagcgacgt
     3001 tgttggtgtc gttagcatcg ccaccgagat cgtttttaat gcggttccag ccggcgtaag
     3061 cggcgaagtt cttgttgatg gagtaaacga ggtttacttc gtgttcatcg ccatcggtct
     3121 tgtaagactt gttgttgtcg ctcttcgtct tcaagccgta gtagttatac tgcaaagccc
     3181 atttgtcgga aaggccgtag ccgaggccgc cctggaagtc ccatttggaa tcagtgtctt
     3241 cctgaccacc tttccattcg tctttggccc agttgctgga atcaaccgaa gctttcgggt
     3301 tccaagcgcc taaatcgagc tgccactgtc cctggttcca ctgcgtctgc ggcgtagcga
     3361 aagcaaagga agtagcgcag gctgccatga ctgcagccag tactaaagat ttctttttca
     3421 tcataatccc agcttcctat tgatatttaa tagtgccacc tatgacatta ttatgttcta
     3481 gtataccata tctttgtcac aagtgcgtca cactatttca tgatttttag tcatatcagg
     3541 actattcccc atacttttat gattattccc ggccgacgta agaaatgatg gcgtccagga
     3601 tatgcggcaa tttccggctc acactgtcga tatggacgcg ttccgtccct tcgtgcaggt
     3661 ccttacccca tgggcccagg aggaggaagg gaatctgtaa ttttttcaga ctgtccaagt
     3721 cgaagggata ggccttgccc cagaggagca ggttcttttc gaccatactt tcatcgaaat
     3781 cggggtcgac gcagcagtag ctgcaatcgg aaacaccgtt gaaatagcgg tcgaagcgga
     3841 catccatcag agtcgggatt ttttccagca ggtccgtaaa ggccggcgtc gggaacgaca
     3901 ggctgtcagc cgccggataa cagggcggcg caaaggcgac gacggccatg ggcgccgtca
     3961 tgcctgtgaa atcgaggatc tgctccaaga gatgtatcgt ctcttcgaca taagtactgc
     4021 tgccttcctg caaggcgacg aaagaagcct gtttggccgc ttcatagaag acgtcgaaac
     4081 cgggataagc cgaagcctgc tgcaagagct catcggcact gcagacggaa acaggcaaat
     4141 cactgcccat ggtctgctgc aggtcgtgga cagcccgccg cgtttcttcc agcaggacgt
     4201 acatgacgtc atcgggcgtc ttgtcgtaag tcagcagatt cagggccgct gccacccgct
     4261 gaggcagggt cacgtcatag cgttccttgc ggtcccgcag atagacccag gcaggcggcg
     4321 gcgtctgttc gccatcacag cagtcagtca ggtccggact gccttccgtc gccgcgatga
     4381 ggtgggccag gatgcccacg ggattgatgc ccttatcata gcagccgatg tggacaggct
     4441 tgccctggac caggacgaca ggctgtatct tgccgaccga accggtatag gcgacgagct
     4501 tgccctgctc cttatggttc ggttcgctgt cgatgaggat gtcataggtc aaatcataag
     4561 aagaacgcag ttcattgagc aaggtaatgg ccgtccgcat gccggccgaa tacgtttctt
     4621 catcgggcag ggataagtaa atcaagctgg cctgtcccgg ctggaaggca tagtcctcaa
     4681 agacggctag ttgcgcggcc gcaccgccct tcatgtcaca ggcaccgcgg ccaaagagcc
     4741 attcgccgct ttcccagtca cggcgcactt cctcactgag cggcgcccgt tccagcgcct
     4801 tgtagagccc ttgcgggtca aaagcctggt cccgcagata gccataggac tcgacatcga
     4861 cgacatcgtg atgattcatg aaaatgaccg tatgggccgt actgccgcgg acgaggccgt
     4921 agacgacaga ccgctggcag ttatcgtcgg gcacatggat gaggccgctg acgacgccgt
     4981 cgaggtgaga gaagaactgc tgcagatagc cttccatctg cctttccttg agcgtgttcg
     5041 tttcactgtc aatatgaatc atatcctgta aaatttccat aatccgcgct gctcgtgcca
     5101 tagagacacc cccttattta aatatccatt ttttacgcga tatataattc caaacggtga
     5161 cgatggcact agccatgatt ttcgacacca tcggattcag gcctaaaata gccggcgatg
     5221 tgaacagcca catgagcagg agattccagc ccaggccaat ggcactgacc aggaagacca
     5281 gggacacttc cgtcccgcga tgatagcggc tgccgctgtc gaagacgaag cggttgcaga
     5341 ggatgtagtg atacagcgtc gacgtggcaa aggccagggt cgtcgccgcc agaacagtct
     5401 gggtctgcat ggaaaagaca gcactggctg acaacagcct caagaaaata taaaagagcg
     5461 tccattccaa taaagccgct gagccgccga cgaggagata catgaagatc ttcatgtact
     5521 cgttgttggt ataatcaaag ccaaaaatct gctttcgttt catggtgttc cttccttttc
     5581 tgttttcgta tccattatac ggcacttgcc agggaattac cacacactgc cgctttttct
     5641 tgcattttct aaaaagacaa tagatatata tacgaaaata tgataaaata tatagtaatt
     5701 ggtaatacga aatattttta gaaggaaggc agatatcctg tggattacac gagatgcatc
     5761 aattttttgc taaccatttc acaacacgaa gtctttttga ttttctccga gaacttgtcg
     5821 gaattcggca tcacgcccgg ccagtacggc gtcttagcct gtctctggaa acatgaaaca
     5881 ctgacgccga aggaaatcgc caacatcctg cgcgtcgaaa actccaccat ttccggcgtc
     5941 ctcgaccgca tgcagaaacg cggcctcatc gaccgcatcc tcgaccccaa caaccgccgc
     6001 agcatccgcg tcaaaccgac ggctgccggc atggccatca aagaaccggt ccagaaacgc
     6061 atcgaagaac tgaatgacat ggtcttcaaa gaattcacac cccaagaacg ggaagaagtg
     6121 ttgaacctgc tcacccgtat cggcaaagtc tgtgccgatg atttgaaaga agggaaatga
     6181 aaaaggggct gtcgcccgtg cgacagcccc ttttgagttt taagtttgta gtttgtagtt
     6241 tttagtgaag gtttttaaat ttaaagtccc tatctacaaa ctctatacta aaaactataa
     6301 agcaaaaggg accgcgcatg atgacatata ccttcatgcg ctagtcccac caaattatac
     6361 aataccagtt aacatgtaga agaatacgcc gaagaaggcg acgactactt tcaagagggt
     6421 aatggcgaac atatccttat aggcttcgcg atgagtcagg ccgcagatag ccagggtcgt
     6481 gatgacagct ccgttatggg gcaggctgtc cataccgccg gaagccatgg ccgcgatacg
     6541 gtggacgact tcagccggga tgccgacggc agccgtttca gccaggaagt gctggccaaa
     6601 gatatcgagg gcgatactca gaccaccgga agcggaaccg gtgataccag ccaggatgtt
     6661 gaccatcaag gcgacgttga acagcggcat gccctgaccg ataccgagga gggcatcagc
     6721 gacttccagg aagcccggca aggacgaaat gacgttgccg tagccaactt ccgatgccgt
     6781 gttcatgata gccagcagag aacctaaagc gccggcattg agagcggctt tgacgctggt
     6841 gttcttggac atgaacttgc ggcccgttgc agcggcgatg atgatagccg ccaagaggcc
     6901 gaggaccagg gcccagatag cgacgacgtt gtggacgcct ttggccagga gaggcacatt
     6961 catggcgcgg aacggttcca gtaaggcatc atcccactgg aaaatcatgg agaagtagaa
     7021 gttcaagacc aggacgacga tgagcgggat gatggacaac tgccagggcg gaagagaagc
     7081 ttccggatcg atgaccgttt cattgagggt atggttgcca tagccttcgc cggaacgggc
     7141 catctgtttg cggcggaatt caagataaat gaagcagagg ataatcagca aggtaccgca
     7201 aatcaagcct aagataggag cggcaaaagt cgtagttccc agataaatcg tcgggatgat
     7261 gttctggatc tgcggcgaac caggatagca gtccatggta gccgtgaaga caccggcgac
     7321 gaagcaggcc ggaatcaggc gtttaggaat atcggcttcg cggaacagtg cagcagcgaa
     7381 gggatagacg gcgaaagcac agacgaacat ggaaatacca ccataacaga ggatcatgca
     7441 ggcgaagacg acggcgaaga cagccttatc tttcccgatg atcttgatgg tttccatggc
     7501 aatactgcgg gccatgcccg tttcttccat gaccttgccg aagacggcac cgaggaggaa
     7561 gaccgggaag aaggacttaa tgtaggtaac ggccttagcc atgaacagtt ccgtataccc
     7621 cggtaaaatc ggcagccccg aaaagctggc ggcaaagagg gcgaaaatcg gtgcgaagag
     7681 aatgaccgag aatccgcgat aagcaaatgt tacgagcaaa aataaactta ggacgattgc
     7741 aacaatatcc atatacgtgt tctccttttc ttttggtttc cgaatgactc atttcgtatc
     7801 ttcatcatac gtgagaacca atgagaataa aagacaacgc cctaagacaa cctttgcgct
     7861 ttttgcccgt tttggaaatt ccatgaatgc aaaaggactt gccgcacatg cgacaagtcc
     7921 cttttgagtg gttagtggct agttgttagt ggttagcgga tggttaaaaa tttaaaaact
     7981 gggattattt cagtttagca gcgacgtctt tggtgatgtc ctgggactgg ccgatgacga
     8041 cggagcccga gaggacgacg tagtccaggc ctttttcttt ctggacggcc tgggtcttag
     8101 cgaggatatc cttttccaag ggcgagaaca gggcaatgcg ctgctggttc agtttcgtat
     8161 tgtatttctg taccagtgcc tgctgcgcgt ttttatcgct ggtctttttc aattctttct
     8221 ggaaatcctg ctgagccgat ttgacatcgg actgccaagt agccatggtc ttcgcgtact
     8281 gcggatgctg ctgcaagagg gcaccggcat tgatcgtgcc caggcccgat gcgaaagaag
     8341 cgccggcagc gctgaaggtc agcatagctg cggctacggt ggtcatcatc attttacgta
     8401 atttcatagt ggttttcctc ctaattattc ttctacgcct gtcagttcca gacattcctt
     8461 gcggacgccg tcttcacaag agcgcatggc taaaatcgtc ttttcaaaga ggtcgttcag
     8521 gtcccatccc agggtttctg ccccctgctt gatgacgtcc cgtgaacagc cggcagcgaa
     8581 gcgcttgtct ttgaatttct tcttgagact cttcacttcc atgtctttcg tgctcttaga
     8641 cggacgcatt ttggcagccg cccagatgag cccggtcaat tcgtcggcag catagaggac
     8701 tttttccatc aagtgctccg gtgcgacatc gacacagatg ccatagccat ggcagacgac
     8761 ggcatggata aaggcatcgt cggcgtcgat ttcagccagt aattccggtg cctttttaca
     8821 gtgctgatcc ggatattttt caaaatccac atcgtggagc aggccgacga gtccccagaa
     8881 atcgacgtca tcgccatagc ccagttcctg ggcataccag cgcatgacgc cttcgacggt
     8941 cagggcatgc atgatgtgaa aaggctcctg attatatttc tgcaataaag ccaatgcttg
     9001 tgagcgttcc atgtctttat ctccccctag caaatctcga tttaccccat tatagcataa
     9061 aaaacgggcc tgactagtct tttttcacga aagcgcctgt atcggtcatc aacgaggcca
     9121 ggcggttcat gcggaagtct tcgacgacct ggatgaaagg ccggtaatgg atgggatttt
     9181 cggcaaaccc gaagagatgg gacgggcagt cgcaatcggt acagccaaag ccctcgatat
     9241 gccagtctgc cacgacatcg acggcccggg ccaggtcgtg ctccaggtca tccgccgtcc
     9301 ggacgggcag ggcggcgacg acgtcggctt cctggcgcag gtagtcgatg tgccagtgca
     9361 tcttcttgcg cttgcggctg tgacgggcca gacgggcagc caggttcttc cgggccgacc
     9421 cggtatagac gtaatagccg gcaggaaaag ggaactcccc tttcgaaccg atggcgatat
     9481 ccttcgcatc tgccaggtga aggatgaata aataatcacc gctgtcatgg ttttcccgtt
     9541 ccaggatggc ctggggatag gtcaggacgc cggctacgcc gggccttgaa aacgagccat
     9601 cccagtgcag ggctagggcc ttccagtcca agtgcggcgc cgcttcataa aaggcctggg
     9661 caaaggccgg atccgtatgg tagtccggca ggaaccagcg ggcccgattc cagtggacca
     9721 ggaacaagac gcccgtgtgg atgccctggc ggctcatggc agctaattcc aagatatgtt
     9781 tgcggccccg ttcggtgacg gcatcgggaa acatggcgcc gtcgcggcta aagagggtac
     9841 acgatttgac ttcgacgaaa aagcgctctt cgccccggcc caggagcagg tcgaaacggc
     9901 tgtcgccgac ggtgatttcc cggcggatga ggtcccagtc ttcccagccc gggatgaggt
     9961 gttcctggac cagccaggct gccgtatcat tattgtactg tgtatcgagc aggataggca
    10021 cgccatcgcg ctcgataccg acgacatcgt aagccgtccg gcgcttggga ttggctgccg
    10081 gccggacata gaccgtcacg ccgggaaaga gcagttccca catgcgcccg ggattgggca
    10141 tatgacaagt cacgacatcg ccatcgagag aaacatgggc gatgaagcgg ttcggcctgt
    10201 cgacaaaggc ccctttgcga atagaaaaca tttccatagg atactcctgt ctgtgaaaaa
    10261 gggcttgtcg cacggcgaca agcccttttt agtggttagg gtttcgtatt ttacagtgta
    10321 ggggtcgcca cgtgggcgcc ccgtcatgga atcgggatga ttctccacgt gcgagcccct
    10381 acgggcggca aacggcggcc tgcgaatgca tgtatgacat tatcgccatc attccgggcg
    10441 ggccacccca cgtgggcgcc cctacgcttc cagccattcg ccggtgaagg tgacggctgc
    10501 cgggccggtc atgtagacgt tgccgtcttg ttcgttgtat tcgatgtgca gcgtgcccag
    10561 gtagagctgg acgtcgacgc tgcggccggt gaagccgttc aggaaggagc cgacggcgca
    10621 ggcgcaggag ccggtaccgc aggccagggt ggcgccggca ccgcgttccc acgtgtggac
    10681 tttgatggtg tggtcgtcga tgacctggac aaagttcatg ttggtcttgc gcgggaaagc
    10741 cttataggtt tcaaatttcg ggcctaattc gtggagatcg acggcgtcga cgtctttgac
    10801 ataggtcatg gtatggggga cgcccatgag caggctggtg atgttatagg tcttaccgtc
    10861 gatttcgatg ggttcatcga tgaccgggcc ggacgggcct tccatgggga tctgggcgcg
    10921 gtccgtgaag ggcttgccca tgttgatggt gaccaggctg accttgccgt cgtcgccgac
    10981 cgtgactttc ggcttcatga tgcctgccag ggtttcaacg gcgaattctt tcttgtcgat
    11041 gatgccgttg tcgtagacgt atttggcaaa gcagcggatg ccgttgccgc acatttccgc
    11101 ttcactgcca tcggcattga tgatgcgcat gcggacgtcg gctttatcgc tcggcacgat
    11161 gacgatgatg ccatcggcac cgatgcccgt atggggagcg cacatcttga cggccagctg
    11221 ggaaaaatcc ttgtccgcgc cgtttttatc ttccaaaaag acgaagtcat tgcctaagcc
    11281 gtgcattttt acgaatttca tagttaaacc ctccgtgaaa aaatatctat actactatca
    11341 ttgcttactt gaatccttac agttgttatt atacaatagc ggaaagtggc aaatatatgt
    11401 agataatgct ctttatattg caaatcctgc tataagataa ggtgtgaggt gacacaggta
    11461 tggcgcaaaa gacgggttat ttagcggaaa aattaaaact cttccatcta tcggctaagc
    11521 ggcagtggac ctgcccctat cattaccacg acttcgacaa gatcaccctc tttttccggg
    11581 gccacgtatc gtatgacgt
//
LOCUS       DAWABZ010000048        11265 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_1588_length_11265_cov_2.919715, whole genome shotgun sequence.
ACCESSION   DAWABZ010000048 DAWABZ010000000
VERSION     DAWABZ010000048.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 11265)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11265)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11265
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1588_length_11265_cov_2.919715"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7471..7480
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2460 a         3313 c         3081 g         2401 t
ORIGIN      
        1 ccttgaacag cccgtccggc gtcccgaagc cggcatgacc gctgacagcc agggtcatat
       61 catcgccgac gagacgcgcc gcttccacag gcgtaataaa ctgtgtcata acgaccttcc
      121 tttcgtgtgg tagtcaaaag gggctatcgc acgtgcgaca accccttttg agttttaagt
      181 ttgcagtttg tagtttttag tgaagccctt tttaatttca aagccctcat ctacaaacta
      241 caaactccat actacaaact ctaaacttta tttcaagtgt ttcaatcctt tggcgccgat
      301 gtcgctgcgg acttgctggc cgtcgaagtg gatgcgtttg acgttggcat aggccttttc
      361 gatggcgctc ttgaggtccc ctgctacggc cgtaacgccg aggacgcggc cgccgttggt
      421 gacgtactgg ccgtctttct tggccgtgcc ggaatggaag accatgacat cttctttatc
      481 gacagcatcg aggccgctga tgacatcgcc tttatgggac gaagcgggat agccggcaga
      541 agccatgatg acgcagcagg atgccgcgtc tttccagtgg accatatcgg ccgtcagcgt
      601 gcccttggcg caggcataca tgatctgggc caggtcgccg tcgaggagcg gcaagacggc
      661 ctgcgtttcg ggatcgccga agcggcagtt gaattcgacg actttcgggc catcggccgt
      721 gatcatcagg ccggcataga ggcagccctg gtaggtgatg ccttcctgtt tgaggccatc
      781 gacgacgggt ttcagaatct tttcttcgac ttccttgcgg atttccggcg taacgaccgg
      841 tgccggcgca taagccccca tgccgcccgt gttggggcct tcatcgttat cgaagatacg
      901 cttgtggtcc tgggcggaaa tcatgggcac gatggtcttg ccatcgacga aagccaggag
      961 gcttgcttct tcgccgacca tacattcttc aatgaagcac cgaagatgtg gtcttccatc
     1021 atggcgttga cggcttcgat ggcttctgct tccgtctggg cgacgacgac gcccttgccg
     1081 gcagccaggc catcggcctt gacgacgatc ggtgcgccct gttccttaat ataggccttg
     1141 gcggcttcgc catcggtaaa ggaggcgtaa gctgccgtcg ggacgtggta tttcttcatg
     1201 aggttcttgg caaagacttt ggacccttcc atctgggctg cggccttgtt cgggccaaag
     1261 acagccaggc ctttggactg ggccaggtcg gcaatgcctt cggtcaaggg gacttccggg
     1321 ccgacgacga gcatgtcgat gccttcgttt ttggcatagt ccgtgatggc gtccaaatcg
     1381 gacagggcga tgtcgacgca ctgggcgact tcactcatag cggcgctgcc gggaatggca
     1441 tagagcttat cgacgctcgg gctctgagcc aatttccagg ccaaagtatg ttcgcggccg
     1501 ccgccgccga tgactgctac tttcattatt ttgcggcccc tttctgaacc tgttctgcca
     1561 ggtatgcggc aatggccgta tcatagacag ccgtgtgatg gaatgcttcc tgagccagtt
     1621 ccaggcggta ctggtcggag aattcgccgt ctttctggag gatggaaatg atttcgccgt
     1681 atttggacgg gttggtgacg atggcgacgt atttgttatt ctttgccgaa gcgcggacca
     1741 tgcagggacc gccgatgtcg atgttttcaa tggcttcagc caaggtgaca tccggtttgg
     1801 caatggtctg ctgaaacgga tagaggttga cgacgaccag gtcgatcggt ttgatgccgt
     1861 gttctttcat ggctttgacg tgttccggat tatcgcggac agccaggata gccccgtgga
     1921 ttttcggatt cagcgtcttg acgcggccgt ccatcatttc cgggaagccc gtgacgtcct
     1981 gaacggcgat gaccggtacg cccgcttctt tcagggtccg catggtgccg cccgtggaaa
     2041 tgatttcaat gccgaggtca gccagggctt tgcccagttc gacgacgccc gttttatcag
     2101 atacactaat gagtgcgcgt tttactttca tgaaaaattc ctcctattcc tgagtccctt
     2161 tgacgacatt gccgtcgata gtcaatttat catcgcaaaa gagcgctgcc gctctgacat
     2221 aagtcggatg ttccttggtg aggatccggg ctgccagcgt gtcttccgta tcgtcttcgt
     2281 agaccggtac ggctgactgc aagatgatcg ggccggcatc catgtcggga acgacgaaat
     2341 ggaccgtgca gccagcaatc ttgacaccgg cttcgacggc ctggcgctgg gcgtggaggc
     2401 cgcggaacga cggcagcagg gccggatgga tgttgagcat cttgtgttca taatgggaaa
     2461 tgaagtcacc gctcaagaga cgcatgaagc cggccaggca gatgatgtcg gcctgatacg
     2521 gttcaatggc gtcgaggata tatttttcaa aggccgcttt cgagtcgaag gatttgcggt
     2581 cggccaaaat gaccggtacg ccccattcct tagcgcggtc catgaccggt gcgcccggga
     2641 tatcgcagac cagggccttg atttccgcat tgatcgtgcc ggcttcgacg gcatcgtgga
     2701 gggcgatggc gttggagcca cggcccgatg cgaaaatgac catcttcttt ttagtcatgg
     2761 aatacgcctc cccgcaaggt tacgggtacg tcggaatcga cgactttgcc gatgacatag
     2821 gccttttcat cgcggtcggc caggttcttg aggacggcat cagcgtcttt ttcatcgaca
     2881 atcatgatca tgccgatgcc catgttgaag gtacggtaca tttctttggc atcgacgttg
     2941 ccccatttct ggaggagtcc gaaaatcggc agctgcggcc acgccgtgac gtcgatatcg
     3001 acgccgacgc ctttcggcag gacgcggggg atgttttcat agaaaccacc gccagtgatg
     3061 tggaccatgc ccttgacggt aaagttcttg atgatcggca ggcacggacg cggatagagg
     3121 cgggtcggcg tgagcaggca ttcaccgatg gtcattccca gttcatcgac gtactggtcg
     3181 agcttcatgt tctgccgttc caggacgatc ttgcggacca gggagaagcc gttggagtgg
     3241 acccctgtcg aaggcaggcc gatgaggacg tcgccggcct tgatgttttc gccggtaatg
     3301 accttgtcgc ggtcgacgat gccgacgccg aagccagcca ggtcatagtc gtctttggca
     3361 tagaagccgg ccatttcagc cgtttcgccg ccgaggagag cgcagcccga ttcctggcag
     3421 gcatgggcga cacctttgac aatggtcgca acctggaccg ggtcgagctt gccgacggcg
     3481 atgtagtcca ggaagaagag cggttcggcg ccctggacga gaacgtcatt gatggacatg
     3541 gcgacgcagt cctgaccgac tgtatcgtgt ttgcccatca tgatggccag gcggagtttc
     3601 gtgccgacgc cgtcggtacc gctgaccagg atcggtttct tgatgtcgct gttcatcagg
     3661 gaaaagaggc cgccaaagcc gccaaggtca ccgaggactt ccggacggta cgtggctttg
     3721 accgaatctt tcatcaattc tacggctttg ttgccagcat cgatgtcgac gccggcatca
     3781 gcataagtca atcctttatt tgtgttcgag agcatatttt tctccttcct cattggcctg
     3841 cggagtgtcc gtaggatagt cgttattgaa gcaagctaag cacatatctt ccttggggat
     3901 atgttcgaca gcccggttga gaccgtcgat ggaaatgaaa tggagtttgt ccacgccgat
     3961 gtatttctga atctgttcga cactgaacgt cgaagcgatg agttccttgc ggaccgacgt
     4021 gtcgatgccg tagaagcagg gatacttgat aggcggtgcc gatacgcaca tgtagacttc
     4081 cttggcgccg gcttcgtgca ggagcttgca gatgatgccg ctcgtcgtgc cgcggacgat
     4141 ggaatcgtcg accatgacga tgcgcttgcc cttgacgacg gacttgacga cattcagctt
     4201 catgcggaca gccagttcgc gctgtttctg gttcggcttg ataaaggtac ggcccatgta
     4261 acggttcttg atgaggccgt gcttgaaggg gatgcccgaa gcctgggcgt agccgatagc
     4321 tgccgtgttg ccgctgtccg ggacggaaat gacgatgtcc gcatcatact gcgtttcgtt
     4381 ccacatttcc cggcccatgt tcaggcgggc ctggtagaca tcctgaccgt cgatgacgct
     4441 gtccggacgg gcgaaataaa tgtattcaaa ggaacagacc tgcttgcgcg gtgccttggc
     4501 aaagatcgtc gatttgacgc ccttttcatt gatctggacg aattcgccgg gctgtacgtc
     4561 gcggacgaac ttggcgccga tggcgtcgag ggcacacgtt tccgaagcca ggacccagcc
     4621 gccttcatcg gtacggccga tgcacagcgg gcggaagcca taggggtcgc ggacaccata
     4681 gagcgtatct ttcgtcatga gtaccagtga gtacgccccc tggatgcggt tcatgctttc
     4741 catgatgcgt tcttcgatgg tgtctttctg gctgcggcta atgaggttga cgatgatttc
     4801 actgtccatg gtcgtctgga aggtcgcgcc gccgcggtcc agttcttccc gcagagtccg
     4861 ggtgttggtc aggttgccgt tatgagccag tgccagctgt cccataggcg tgtatacggc
     4921 caggggctgg atattgcggg gattattgga acctgtcgtc gaatagcgga cgtggccgat
     4981 gccgacgggg ccgccttctt cttcaggtac gccgttggcg aagaccgtgc tgatgaggcc
     5041 catgccgcgg aacgtatgga tatcctggcc gtcagtcagg gcgatgccgc cgctttcctg
     5101 gccacgatgc tgtaatgcga acaaccccca gtaaatatag cggggaatat cgaggtgttt
     5161 atcgtaaata cctaagacac cacattcttc atgctgctta tcccaaatcg gatcgtaaaa
     5221 catgaattaa acctcctgtt ataacgaaaa atagtctgta ttgcccttag agggacttta
     5281 tggtttcttg caacttctgg ttcttttcca tgacttcgtt agccatgttt tcgcgatagg
     5341 ccttgtattt cttggctaag gccgcatcgc tggcagcgcc gatctgtacg gccaggagcg
     5401 ctccgttctt ggcaccgttg atggcgacgg tcgcaacggg gatgcccgac ggcatctgta
     5461 cgatggccag cagcgcatcc atgccctgga ggctgccgct ttccaagggg acgccgatga
     5521 caggcagcgt cgtatagctg gcaacgacac cgggcagatg agcagccata ccgggaccga
     5581 cgacaaaggc actgatgccc cgttcttcgg ctgtcgaaac gaattcccgg acagcagccg
     5641 gcgtgcggtg ggccgacgct aacaagactt catattcaac accaaattct ttaaaaatag
     5701 caatcgcttt tttcataacg ggaaaatcag aatcgcttcc catgatcaaa ccgaccttca
     5761 acagagaccc ctccttagga aaaacaaaac ccaaaagtga cacacgtcac ctttgggctt
     5821 ttttcagtta gacacacgga gactgtttac aaacggcaaa gaacctgtcc ataaacgtct
     5881 tgtcatagtg catctcctta tcaaatacac tttgattcta tcaaaaacat ccgggactgt
     5941 caatgaaaat cacccgttct atcgcagttt gtacacggat agcgccgact atgttacaat
     6001 ggaggaaaat gaccgacacg gagggataga ttatggaatt aaatgaccag cagcagcaag
     6061 tcgtcagcga cctgtcgcag ccgattctgc tcaacgcgcc agccggcacg ggcaagaccc
     6121 gcgtcctggc ctgccgggtc gcccacatcc tggaaacagg cgccgccgaa ggctcgcaga
     6181 tactctgcct gaccttcacc aaccgggcct gcaaagaact gaagaaccgc atcatcgcca
     6241 cggcccgcga aaagggcctg gctgtcgtcg tcaagacaat ccacagtttc tgttacagcc
     6301 tcatcaaaga agaaacgaag cgccagagcg accgttacaa cgactttctg ctctacgacg
     6361 acgaagactg ccggcagatc atcgacagcc tgcctgcggc agcgtcagcc ccgggcaacg
     6421 acacccaggc cctgcagaac ttcatcgaat ttgtcaagaa acagcgcgtc atcccggacc
     6481 tccacgattt cacggcacct aaagaagccc tccaggcctg gctcagcgac cacggcgacg
     6541 ccctggtcca gcaatacgat gccgtcctgg ccgacaacca cgccgtcgac ttcacggacc
     6601 tcatcaccgg cgcctacgac ttcctcactg acgatgcctg ctgccagcgc tggcgggacc
     6661 gcttccgctt catcgccgtc gacgaaatgc aggacacgag cgaagtcgaa tacgccgtca
     6721 tcagccgcct cttcccgggg cgcaacgtcc tcctctgcgg cgattacttc cagaccattt
     6781 acgaatggcg cggttcttat ccggaatata ttctggaaca attccgcaag acctaccatc
     6841 cccgggaaat cacctttacg accaactacc gggctacgca gctcctcctg aaggccgccg
     6901 aaagctgcct gaaccacctc ttcggccagg tgacggtcca ccacatctac ccccagccgg
     6961 gccaggccgc agccgccgtc ccaggtgaac ccatcgtcgt ccaccaggcc gatacctttc
     7021 tcaacgaaag ccgctggatt ttccaggaac tgagccagct gccgcctgaa gagcgtctca
     7081 aagcctgcat cctcgtccgc ggcaatcgcc aggcccagca gctgtggaca ggtattacgg
     7141 cccataatct ccactatccg ccagcatccc gtctgcccgt cacctgcatc gaccagtttc
     7201 acctgttcaa gcgccaggaa tgtaaagacg tcctggccta cctgcggctg ctcctgaaca
     7261 agcacgacaa cctcagcctg aaacgcatcc tcctgcgctt cgtcccccgc atcggccggc
     7321 ggaccatcga aaccatcgaa agcgagtcct accgccgtct gggcctccgg ctataccggt
     7381 gccgggcgga aaatcgccgt cattgatacc ggcatcgatg tggaccaccc ggctctggac
     7441 ccggatgcct tggcttacgc cctggagggg nnnnnnnnnn tgacggccct ggcagatgga
     7501 cgcatcgtcg tcttcgacgt cgaaagcacg gggaccgaca cgacgacaga cgaaatcatc
     7561 cagattgccg ccgtccgcct tgacgcccag ggccagatca cagacaagtt caatacctac
     7621 gtccgcgcca gccgttctgt cgggacctca ttctacgtcc atcacatcag cgacgaccag
     7681 ttagccgccg atggccggga tccgaaagaa gccctgtctg cctttctgga ctttgccaga
     7741 gatgccgtca tcgtcggcca caacgtcacg tatgacctgt ccatcctgca cagcgagctc
     7801 cagcgcctgg gcatggccga aagcggtgac ttcccctatt acgacacgct gggcatggcc
     7861 gaaagcggtg acttccccta ttacgacacg ctggacatat accgccgctt ctatcccaat
     7921 cttatcaacc ataaactggc tacgctgagc gaaaccttcc ccatcgacca tcagccgtcg
     7981 cacgacgcct tcgacgacat tctggccacg gccggcctcc tgtgctacgc cgtaaaagaa
     8041 aagatagcgc cggccacggc agcccgccgc gcttccctgt ctatgtacct gcccctcttc
     8101 gctcctttca gccgcgacat gggccgtttc cgccgccaat cttaccagga ccggcccata
     8161 gccatcatcg ccgccgtcat gaacgaaggc ggtgtcaagg cctggtacga gagccatcgt
     8221 gaggccagcg atacggccca tcacatcgac cgcctggaaa acgtccgcaa gctctaccgc
     8281 atcgccaagg aaacggatga cccggcccag gacccccgcg acgccctggc ggaatttctc
     8341 aagctgacag ccctgtccaa cagcgaactg gacagcatgc tggccaggga cccgaaaatc
     8401 ccgatcatca ccatccatca ggccaaggga ctggaattcg actacgtctt cctggccagc
     8461 ctggaagacc gggtcttccc gaccttctgg tccgtaaaga aaggcaatat cgaagaagaa
     8521 aaacgcctct tttatgtcgc cattacgcgg gccaaaaaga ggctgtatct gagctggcac
     8581 cagggaatcg gcaatcagcg ctatgcgccg agccgcttcc tccagagcct ggactcccaa
     8641 tatatcgaag ttagatgagg ccggctatgc ctaagaggag gcctaagacg ccggatccga
     8701 tgaggacctt gatgacgccg attttgcgcc gcgtcgctgc aatagccaag aagaggatga
     8761 ccaagccgcc aaggctgaaa gccgaccagt catcaggcca ggtctccgta ttccacaagg
     8821 ccaggaggat gaaactcaga ctggcgacgc agataagggc gacgactgcc ggccgcaggc
     8881 cgttgaggat gccccggata ggaccgatat caccgtactt gaggaagacg cgtgctaaca
     8941 gaatccccaa gaaaaacgac ggcgtgacga agcccagggt ggcagccaca gctccgccta
     9001 atccggcgat tttggtgccg acgaaggtcg cggcattgat gccgatcggc cccggagtca
     9061 tctgagaaat ggtgaatata tcgacaaatt cgctcatcga gagccaatgg aaggtatcga
     9121 cgacactggc ctgaatcagc ggcatagacg catagccgcc gccgacgcag aaagccccga
     9181 ctttacagaa ttcccaaaaa agcaataaat atatcatgat aagtccctcc ccttaatcgt
     9241 acttcttgtc gcgcatgaag gcaaagccaa tgatgccgtc gatgacgatg agcgccatga
     9301 tattgacatc aaagcataag ttagcaataa acgtcgctac gatgatagcc agcggcagga
     9361 ggagcttctt ttttccctgt ttgatgagga ggtcgatgcc gacattgacg ataagcgccg
     9421 tcgccccgca ctgcataccg cggaggatca tcttgatata gagattctgg ataaagaaat
     9481 catagcagta agaaataatc gacagcgtaa tcaggcaggg cagcaccgtc gcgaccaggg
     9541 cgaccagcgt ccccaggacc ccggccatgc ggtagccgag gatgaccgca ctgttgaccg
     9601 caatgacgcc aggcatggac tgggcgattg ccaccatatc gagggtatcc ttatcgctga
     9661 tccagtgata ttcatcgacg tatttccctt tcaaaagagg aataatgacg aagccgccgc
     9721 ctacggtgaa ggcgctgatt aaaaaagtcg atttgaacag ggtccaaaaa aaatgggcgt
     9781 cccgtttcgg tgtcacaata tcgttcatca tctatcatcc tttcttgacg atcatcttgt
     9841 ctatggtctt agtataacac ggcctttact atattgaaaa tatttgtatg attgatataa
     9901 tatacgtaaa ttatatgtca aggaggcatc gatatggata tccgtcaact tacgtacttc
     9961 atggtcatcg ccgaagaagg tcagattacg gcggcagccc gtcgcctgca catggctcag
    10021 ccaccgttga gccagcaaat gaaagctctg gaagaagaac tgggcgtcca gctcctgcag
    10081 cgggaaccgc gcagcgtgac cttgaccgac gcgggcgaaa tcctctaccg ccgggcccgt
    10141 cagattctta ccctcaccga ctcgacacga cgggaaatcg ccgatttcaa gaatggtctg
    10201 cgcggcacct tgtccatcgg gaccgtgtcc tcgtcgggca gcgtcattct ccggccagcg
    10261 ctccgccaat tccacgccag tcatcggggc atccgctttg aaatctatga cggcaacacc
    10321 ttccgcgtcc tggacatgct gggcaaaggt ctcatcgaaa tcggcatcgt ccgcacgccc
    10381 ttcaagcagg acgccttcga ctgcaccttg ctgcctgaag aaccgatggt cctggcctat
    10441 gccgataccc tggccgaccc cagcccgggc cagccgaccc tgaccataga acaactgcaa
    10501 ggcctgccgc tcatcctcta ccgccgcttc gaccagctct tccacgacgt ctgcgaagcc
    10561 cacaacctca cgccgaacct cctctgccgc aatgacgacg cccggacgac cctgctctgg
    10621 gccgaaaccg gcctgggcta cgccgtcgtc ccggcatcgg ccatctcgcc ggcccgcctg
    10681 ccccagctcc agaccaaagc catcgacgaa ccgcgcctgc gcacccaact ggccgtcatc
    10741 acacccaaac accgctacct gtccagcata gcccgccagt tcatagatgc tttgacaaat
    10801 cctgaggcct gacgacgggg cataaaaccg ttcatcgtag gtcgttcgtc gttcatcgcg
    10861 gttggggcct gacggccccg caggccgccg ttcgcaggcc acctcgggct cagatgccgc
    10921 tgcgcggcaa cagattcagc gtaacaacat cacctcgagt gaccctcggc tcgtggaaaa
    10981 ggccgtggtt cgtggctcgt gattcgtggt tcgtaaaaag cctgccatta tacccggatg
    11041 ggctgtgatg aaatctctct ggcgggctgc ccactaggac agcccctaca aatgcggcat
    11101 acagccacat aaaattttat cgcctgtatg cgataccgta ggggacggct aaaagccgtc
    11161 cagcttccgc tggtcggcta cctgacgggc caaaaaggcc gcgcgaattc tgtacacaac
    11221 cggaatttat gacgaacagc acatggtcat aatacaatca tgagt
//
LOCUS       DAWABZ010000049        11251 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_1593_length_11251_cov_3.076635, whole genome shotgun sequence.
ACCESSION   DAWABZ010000049 DAWABZ010000000
VERSION     DAWABZ010000049.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 11251)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11251)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11251
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1593_length_11251_cov_3.076635"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    636..645
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2396..2405
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2779..2788
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2643 a         2965 c         3200 g         2413 t
ORIGIN      
        1 ttttcactgc gtattttcac cgtattcgtt atagagttgt ttgaggatgt tctttcgttc
       61 taccatgccc aggagcttct tgtccttgac gatggggatg gctttcaggt gttctgcgac
      121 catgacgctg gccaggtctt ccatggtggc gtccgggccg gccatgatga cttttttggt
      181 catgagttgt tcggccgtgc aggcaaagag tttcttgaag ccctgctggt attccttgag
      241 gttgccgtaa tagatgttgg ctcccaggag gttgacgtat tgggggacac agggcttgac
      301 ttccttgtac aggaggtctg cgtcggtgac gatgccgatg atttccttgt cgtctttgat
      361 gataggaatg gccgtgatgt tgttttctac gaacatatcg acgaggtcaa agatagacgt
      421 atctttctgc gccgtaatga catttttttc catgatatct gctgccgttt tcgacataag
      481 atcaacccct ttatcataaa ttcgatggga gcatgactcc tattcaatta cattatacca
      541 tattttgggc gtatagggac gtctgttgcc gtcccgccgg tcgctatccg ctaacctctt
      601 aaacgggctt cccacgtgga agcccctaca gcggcnnnnn nnnnnagatt atttagtagc
      661 cgaccctagc ggcaatacga cgattctggt gctgacgcca gtgccgaaag aggaacacag
      721 tgctttggcg gcgaagttat tggccctgcc gggcatcgaa gcggaacagg tcggctatgt
      781 gacccgcgaa gcgggcaagc ccctgcgcgt cgacatgatg ggcggcgagt tctgcggcaa
      841 tgcatcgcgg tcggcggcgg cctgggccct ggcctgtgac ggcgggacac aaggcgtata
      901 cgatgtatcc tgctcgggct gcgatacggt cctgccggct aaggttgccc agaaaggcga
      961 tggcctgtat gaagccttca ttgaaatgcc ttatcccgaa gatgtgagcg gcgtcctcgt
     1021 cgacgccggc gatgtgccgg cccgtttttt ccgcgtcgat ttgccgggga ttacgcactt
     1081 cgtccacttc gtgcctgatt tagaaggcat tgataaagag aaatactggc atatcctggc
     1141 tgattacgtc gatggcgagg atttcccggc ttacggcctg attctctgcg atacgaagga
     1201 acagaccatg attcccgctg tctacgtccg cgatacggac acactatatt gggaaaacag
     1261 ctgcggcagc ggatcggcag ccgtcgccgc ggccctggcc tgcacgaccc ataagaacgt
     1321 ggcctgctac atgaagcagc ccggcggtac cctggccatt gccgccaaag tcggcgaaca
     1381 aggggaactg cagcagatct tcatcggcgg tcccgtcaaa ttgagccaag tccaaaaggc
     1441 aactatatga tgaacgataa cgggattccc acaacgggcc gccttaacag gacggcccct
     1501 acagtgcgac atctatgatg tcgctgcggc ctgcggcttg cgaactgcgg cctgcccggg
     1561 acgggcgccc ctctacaccc gcggtccagc ctggatgatt tcttcgggca tgtcggggta
     1621 cttggtcttg aagttttcgt agaacatggc ggcgactttc ttggccatga tgtcgtagga
     1681 cttcttgttg tcccacgtcg cccgcgggtt gagtatctgg ttgggcacgc ctgggcagac
     1741 ctgggggatg tcgacgttga agcgcatgtc gtgacggtag gggacgttgt cgagttggcc
     1801 gttcaaggcg gcggtgatca tggcccgtgt gtatttcagg tccatgcggc tgccgatgcc
     1861 gtaggggctg ccggtccagc ccgtattgac caggtagacc ttggtgttgt acttttcgat
     1921 gcgttcgccg agcatgccgg cgtaaatatc cggcgccaag ggcataaagg gttcgccgaa
     1981 gagggtcgag aaggtcggaa tcggttcggt aatgccctgt tccgtgccgg cgactttcga
     2041 cgtgaatccc gtgacgaagt ggtacatcgc cgcttcttta ctgagccgcg aaatgggcgg
     2101 caagacgccg aagctgtcgg ccgtgaggaa gatgatgacc gtcgggatgc cgcctttgcc
     2161 gggctgggcc gcattgggga tgtagtcgat agggtagccg gcccgcgtat tttcggttat
     2221 cttgctgtcg aagtagttgg gatggcgcgt gtcttcgtcg aggacgacgt tttccacgag
     2281 cgtgccggcc ttgatggcat tgtagatata cggttccttt tcgggattga ggttgatgca
     2341 tttggcgtaa cagccgcctt caaaattgaa gatgccccgc ggcgacagcc gtgttnnnnn
     2401 nnnnngcgcc ggcaaagccg tcgaagaggt aaatgggccg gttctggaag taggccagga
     2461 ttttgctctt gagggcgttg aatttttctt tggaaatggg ccggttgatg ttgttccagg
     2521 cgatgtagtc gtggatggac ggcgtatcga cgatgaactt gtcatccggt gcccggccgg
     2581 tatatttgcc ggtccgcacg accagggccc cagtgtcgct gaggacgcct tcgccattta
     2641 acagggcctg ttccgtcagc caggccggcg acatattgcg gtatactact ttgggattca
     2701 tgagtcctat gcgttctaaa ccatatgtct gttgcatatc tatcactcct ttgagatttg
     2761 tggctcgtag gggccgccnn nnnnnnnntc agctgcgctg acagctctcc tagcaggaga
     2821 gccgggccgc cttgacagga cggcccctac ggcgaactac ggcctgcggt ctgcgaacac
     2881 ctatctcttt taatcatact aacgtactaa atatagtata atacaatctg gtacactatt
     2941 tcaaattgca tactggatac acgataaagg tcaaattctt aaaaaatact tgactatttg
     3001 actttttgct gtatgatatt ggtgtaaggg tacctgaagg tatccgccac tacggcagaa
     3061 tcgtacaaag gagtgtttga atatggcaaa agaaacccat gaatttcaag ctgaaacgaa
     3121 acaattactg gacttgatgg tccattctat ttatacgaac cacgaaatct tccttcgcga
     3181 actgatttcc aatgcatccg atgccatcga caagctgcat taccagtccc tgaccaatgg
     3241 cgatctcctc gaaggcgata cggcctttga aatccatttg acgcctgatg ccgatgccca
     3301 cacgttgacg atctccgata acggcctggg catgaataaa caggatttga tggaaaacct
     3361 cggtactatc gcccagtccg gcaccaaggc attcctggaa aaattgaaag aatcccagga
     3421 tgccgacagc acggataagg atctcatcgg tcagttcggt gtcggtttct attcggcctt
     3481 catggtcgcc gataaagtca cggtcgtcac ccgcaaggcc ggcgaagaac aggcttataa
     3541 atgggaatcc actggcgacg gtgaatacag cctggaagaa tgtcagaaac cgaaacacgg
     3601 cacgacgatc atcctcagcc tgggcaagga cttctacggc gacgacagtg aagaaaacta
     3661 taccgacaag atgaccctcc agagcctggt caagaagtat tccgactata tccgctaccc
     3721 gatcaccatg gacttcacga ccaaggaaaa accgaaagac gccgacggca aggtcatcga
     3781 agacgcaccg gaagaagaac atacggaaac gcggacgctg aactccatgc agcctttgtg
     3841 gacgcgcaat aaatcggaca tcaaaccgga agaatataag gaattcttcc agcatcagtt
     3901 ctacgaatgg gaagaaccga tggaaatctt ccacaaccgc gtcgaagggg ccgtcgatta
     3961 cacggcactg ctcttcatcc caggcaaggc tccgttcaac ctctactatg ccgactacga
     4021 accgggcatc cagctctatt cgcgccacgt cttcatcatg gataaatgca aggacctcct
     4081 gccggattac ctgcgcttcg tcaaaggcct cgtcgattcg ccggacctgt cgttgaacat
     4141 ttcccgtgaa ctgttgcaga agagccgcga attgagcctc atcggccgta acctggaaaa
     4201 gaccatcctc aagacgttga aacgcaacct ggaaaaggac cgtgaaaaat acgaaaaatt
     4261 ctgggctgaa tacggcaaat ccctgaaaat cggcgtttac agcggtatga tgaccggtga
     4321 aaacaacgtc gaaaaattga aggacctgct gctcttcatg tcatcgaaag atggcaaact
     4381 gtgcaccatc aaagaatacg tcgaccgcat gaaagacggc cagcagaaga tctactacgc
     4441 aacgggcaag gataaagaag ccatcgaagg cctgccgcag atggaaatgc tccgcgaccg
     4501 cggcatcgaa gtcctctaca tgaccgaccc ggtcgatgaa ttcgccgtcg aagccatcgg
     4561 cgaatacgaa ggccaccgct tccactccat cagccgcggc gatctggacc tggaagacga
     4621 cgccttcaag gaagaaaaga agaagaacga agaactggcc aaggataatg aaggcctctt
     4681 gaaggacatg aaagaattcc tgggcgataa agtcgccgat gtccgcttgt ccaaccgctt
     4741 gaagagcggt gccgtctgcc tcgtcgccga cgctgccggc ccgtcgctgg ccatggaaca
     4801 ggcctttgcc gctgccaaca accccatgct caaggcccgc cgcatcctgg aaatcaaccc
     4861 gcagcatgaa ctgttcagca aactgacggc cgtccacgac cagggcaaag acagccaatc
     4921 cttcaaggac tactgcaatc tgctctacga tcaggctctg ctcctggaag gcatcatgcc
     4981 cgacgacccc gtcgcctttg ccaataaagt agccagcctg atggcaaaat aaaattccat
     5041 acagaaaagc ccctcccggt gtgtgaccgg aagggacgtt cctccagcaa aaggtcctgt
     5101 cgttagacga caggaccttt tgccgtaggg gccgtcccaa gcctttccag cccgtcaggt
     5161 gccaaccaac gggaggcgca ggacggttgc tggaaaggac agctgtggct gggcggcccg
     5221 cttgtgggaa ttccgcaaac tgatactgca gttcgtaggc ggcaaatcat cgattaacct
     5281 ctcagtcagc tgcgctgaca gctctcctag caggagagcc gggctgccat aggggccgcg
     5341 agccacgagc cacaaactgc taaccgctaa cccctaaccc ctagtggaat atgttgatga
     5401 acacggtgat gatcgatgcg ttgactgggt cgatgatgag cgagccgatg agggggatga
     5461 cgaagaaggc cgtcggtgcc gggccgaaga gttcggtcat ggctgtcatg ttggcgatgg
     5521 cgttcggcgt agcgccgagg ccgaagccgc agacgccgct ggtcatgacg gctgcttcgt
     5581 agtccttgcc catggcgcgg aagacgatga agtaagcgaa gatggccatg aacagggcct
     5641 ggctgaggag catgacgacc agggggatgg ccaggtcggc gagctgccag agcttgaggc
     5701 tcatgaggac gcaggtcaaa aagacggtca ggctcaggtt gcccaggact tcgctttcgc
     5761 gctgatagat tttataggcg tgcgtgaagt cggcgatgtt gcggatgatg gcggcgacga
     5821 tcatggagcc gatatagccc gggaagacca ggccgatgct gctgaagaag ccgctgacca
     5881 gagtgcccag gcccatggcc aggaagaggt gggccatggc gttcatgaaa cggtagttgt
     5941 cgatggtctg gccgatttta ttttcaatct taatggcgtt ttcggcgcgt tctttattgc
     6001 cggcgacggc tttgagcgtc gaaacggtgc cggcattgaa ggacgggtcg aagcgttccg
     6061 attcggccga gctgacgagg tggaatttgt ggaccagttc ttcgccaatc gggccgccga
     6121 tgacgccgcc ggcgacgagg ccgaacgtag ctgctgcgac ggcaatggtc acgccgccgt
     6181 cgaggccgat gctttccagg agcgggccga aagccgctgc cgtaccgtgg ccgccgacca
     6241 tggggatgga acccgttgcc aggcccagga gcgggctcag gttaaaggcc atagccaggc
     6301 cggagccgat gatgttctgg cagatgatca ggaccgtcgt gacaatggcc attttcagga
     6361 ccatgacgcc gccgcgcttg atgaggctga tgctggccgt atagccgacg ctggtgaaga
     6421 agagcatcat gcagatgttc tgcatgatcg tatcctgttc gtatttccag atgccctgcg
     6481 tatataagat gcaattgatg atagcaaata agataccgcc gacgacgggc gccgggatac
     6541 agtacttacg aaggacgctg atcttgacgc gcaggaagac gccaacgtag tagacgacga
     6601 tggccgcagc cagggtctga taaatattaa gatttaaaga cacacggaaa acctccttat
     6661 aattttaaaa atacaataca tatcataaca gattttaggc tgtaaaacaa ttgtaaacat
     6721 gagaaaatgt tttgattttt taagcatata tgggatatta ttttttaata gacagaaaaa
     6781 atggtgaatg ataataatct tgacggcctt ttcgctggct ctctttagaa aaacaaaaga
     6841 actggatgtc gctggccttg aggtccagct tcagctgttc ctgtcccagg ggagagaggc
     6901 cgggagcgtc ggaacgcact ttggtgacca ccgggagctg gcccttggcc cctttcagga
     6961 aagctgcacc gcggctgttg agggccagga ggcgggcata ttgcggacct gcctcatagg
     7021 agcagtccat gaggtcccgc gagggctgca agagcgtata ggcgcccatg cggcagaggc
     7081 ggctgtaggc atagcgccgc gtcttgatgc tcctgagggc ctggtcgtaa ctgccgcttt
     7141 ccatgaaagc gcggtgccag cggttttcca atccttccgt aaaggccggc agactgcgca
     7201 gctggtccgg tgtcagcagg cggttatggc tggcgacgaa atcgccgtag cggccgtaat
     7261 cagtgaaggc gccctgggcc agctggtctt cggcacctgc caggctggcc gctgtgagat
     7321 aaggccgaca ggcggcggct gtcccgctgc agcgcatggc ctggcgcagg gccgaggcgc
     7381 tggcaaaggc ggcaccgagg ccggtatcgc cgtgtcggcc ctggcggggc aggggaagaa
     7441 aggtcaaggg ctgttcctgt ttcaacatgg cccgggcata ttcaaaggcc agcagggcat
     7501 tgggctgctg cagcagccgg gcttcgtcag gggccgcggc catgagtgcg tcagtcagct
     7561 gctgtccggc tgaggatttt tttgctgcct tcaggcaggc tgtcagtccc tcggagcggc
     7621 gggccaggga gagaaagtca gccgccgttc cgccttcgac gccgcaggcc agggcctggc
     7681 agcccaggcg ggcggctaag gtgacgccgc cttgagcgaa accggccgcg ctggacaggg
     7741 catagacggc agggagttcg atgaccacat cggcaccgcc gtcgatggcc caggccgcgc
     7801 ggtgccattt atcgaaaaaa gccggttcgc cgcgctggac gaaggcgccg ctcatgatga
     7861 tgacgatggc cgcctccggg ccggctgcct ggcggacttg gcggattaaa cgggcgtgac
     7921 ccgtgtggaa cgggttgaat tcggcgatga tggcaaggga ttccatagag cctcctatat
     7981 gttcagaatt tcacaaaatc tcgaaaaagt atagcctgat gagaattata ttaatagcct
     8041 gatgagaatt atattatatg ttttaaataa aatcgtgcta atggttataa gtatacgggg
     8101 caggccggga aacgtcaatg gtgtatactc attcctgcca agggagaggc gaaagtattt
     8161 gcaattcgtc gctttttttt aggtgacgct ataaatcttt tatgctataa tgaacctagt
     8221 ctgtagaaag cataaatagg tgattgcatg aaaggattga aattattagc agttgcggcc
     8281 atgaccgcag ctgtcgtatt gttgtccgga tgcggcaaca tgacgatgct cgaagaatac
     8341 agcttcgggt atgtccagct caaagccggg aattcggaag tctatgtcat gacaccgttc
     8401 gacctggccc atgtaagccg ccagaatggg gaaggcatga tgtacatcaa caacgataag
     8461 cacatcaatg tcgtcgctgt ttccgaaacg gctggcgaaa cgacgcctgt agccatggca
     8521 gaagaatatg ttgccatgct gaaacagaca ccggatgttt ctgatctgca aacgaaggtc
     8581 accgacgcgg aagaaagcgg ccgcaagacc accgttgtcg acgcttccta tacggaaccg
     8641 ctcaacggcc agccggccaa actcatcgtg cggagtctct tctttgaaga taatggccag
     8701 atatggcacg tcatgtacat gtacaaagac ggcgatgccc tggctaaaga agtagttgac
     8761 cacgtatttg gccagctcaa ataattctaa gacaaatgaa agagggtaaa ttttcatgat
     8821 cgtattggtt gtaaactgcg gcagctcgtc cttgaagtac cagctcatca acatggacaa
     8881 tgaagaagta atggcaaaag gtttggtcga aaaaatcggc cttcccgatt cgcagctgac
     8941 ccacaaatgg aatggccaga agaaagaaat caaacagtcc atcccggatc accaagtcgc
     9001 tgtcaaactc gtcctcgaca tcctcaccga cgctgaatgc ggcgtcatca aatccatgga
     9061 tgccatcgac gctgtcggcc accgcgtcgt ccatggcggc gaagaattcg ctgcttccac
     9121 gctcattacg gatgaagtca tgaaagcctt ggaaaaatgc tcggctatgg ctccgctcca
     9181 caatccgcct aacatcatcg gcatcaacgc ctgcaaagcc atcatgcccg gcgttcctca
     9241 ggtcggcgtc ttcgatacgg ctttccatca gaccatgccg gcgaaagcct tcatgtatgg
     9301 cctcccgtat gaactctaca aagaagacca catccgccgt tatggcttcc acggcacgag
     9361 ccatcgctat gtagctggcg aagtcgctaa agtcatgggc gtacccgttg aaaaactgcg
     9421 catcatcaac tgccacctcg gcaacggttc ttccctggct gccatcaaat atggcaaatg
     9481 cgtcgacacg accatgggct tcacaccgct cgccggcgtc ctcatgggca cccgctgcgg
     9541 cgatatcgac ccggctatcg tcctcaacgt catggacaac cacaaccttt cgacgaaaga
     9601 aatggatacg ctcatgaata agaaatccgg cgtcctcggc atttccggcg tttccagcga
     9661 cttccgcgac ctcggccagg ctgctgctga aggcaacgaa cgcgctcagc tcgccctcga
     9721 catgttccac tatcaggtcc gcaaggaaat cggcgctttc gcagctgcca tgggcggcgt
     9781 agacgtcatc acctttacgg ctggcgtcgg cgaaaacggc atcgaagacc gtgcagccat
     9841 cgctgaaggc ctggaatacc tcggtgcgaa actcgacccg cagcgcaaca acgtccgcgg
     9901 caaagatgcc ctcatcagca cggatgattc gacggtcaag atgtacgtca tcccgacgaa
     9961 cgaagaaatc atgatcgccc gcgatacgaa ggacatcgta tccaaacagt aatgaccaaa
    10021 gtgcgtgccc gaagatggag tgaatactgt gaaattacta tactgggaca ttgacggaac
    10081 cttattgaat acgggccggg ccggtctgta cgccattgaa gaagtcttcc acgacctcaa
    10141 gggcgccgac gtgcccgtgc cgaagatcgc cgccggcggc cggacggata atttcatctg
    10201 ccagcagctc ttgtataaag ccacaggcat catgccgaca gacgatgaag tccatgcctt
    10261 ttgccggggc tatgaaaaga agctcctgaa atggctgaag cgcaaggccg aagagggcgt
    10321 tgtcctgccc cacgtagaag agaacctggc tttcttccaa cagcgaaagg attacaggca
    10381 gctcctcctg accggcaaca gcgccatcgg cgcccgcatg aagatggacg tcttcggcct
    10441 gtcccagtac ttcgatttct ctcattctgg gttcgccttc aattattact atcgcgatga
    10501 tctggcccgc aatgccctgg ccatcgccaa ggaagcctgg ggcgatgcca tcgagatggc
    10561 ttatgtcatc ggcgacacgc cttacgacat ccagtgcggc aaagccatcg gcgcccgcac
    10621 gatcagcgtg gctacaggcc gctattccta tgaggaactg gcccagtgcg cgccctggaa
    10681 gatactgcgg tccctgccgg aaccggaagc cttcctgaaa atcatcgaaa attaaagaaa
    10741 agtgtaaaaa ataggctgtc acattaagca aaacatgcat taatgtgaca gcctattttt
    10801 tgagttttaa gtttttagtt tgcagtgaat ggctgaaaaa attaaaacca ccggctacaa
    10861 actgcaaact ccaaactata aagcgtccgg gtctttttcc ggttcttcga tgcgccaggt
    10921 gttttcgagg acgcggaaat tgaggcggtg ccatttcttt tcgatatcgt ccagtgtctg
    10981 cgaggcggaa tataacatat tattttgttt cttggcctgc ttttccagct gtgccatgcg
    11041 aacgcgctgg gtggatacgg tgtccgccag ttcatagacc tggaactgca aggcggcgat
    11101 ccagaagaat tccagcaggg cgacgatgac gacgcatttc tgggcctttg gcagaccgtg
    11161 ccaggcctgg cagaagcggc ggcgcaggcg ggagagggcc gcccggccgt gctgctcctt
    11221 ttggcggatc cccgtcaggt caggagggaa c
//
LOCUS       DAWABZ010000050        11171 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_1609_length_11171_cov_2.972562, whole genome shotgun sequence.
ACCESSION   DAWABZ010000050 DAWABZ010000000
VERSION     DAWABZ010000050.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 11171)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11171)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11171
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1609_length_11171_cov_2.972562"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8512..8521
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2309 a         2919 c         3016 g         2917 t
ORIGIN      
        1 gtctaaatgc cgttcttccg caatctggcg ggccgaacgg agagacataa caccgagctg
       61 ttcgttagaa tcactgacca cgcgtacttc acgggctctg atttgatcgt tgatacgtaa
      121 ttccttacta atcgtctttc acctccgaaa taaaaaagaa cggatagcat cgctatccgc
      181 tctacgtttc cgtcatagac cttagtgact ccagcgcctt gtaaggtgag aagcggatag
      241 cttctgcttt attttcaaca gtacatatta tatcagcctt cacaggcctt gtcaagatta
      301 tttaactttg acgaagcggt aatcctgttc ggcacgggga tatttttcct tatcgacttc
      361 gctcatgaaa tccttcagcg gacgggcata ggtcttcatc gtgtcgtgca aggctttgta
      421 aatgacgaat ttttcacctg tctccgtatg ttcggcaatg gtaataattt cataggtatt
      481 cttcttgaag tggcgccaaa tttcatgtgc tttcggtaat tctctcatcg ataaccctcc
      541 taataatcta cattactaac ctggtgtaat tataccatag tttctcttta tttcaatggc
      601 ttcatgatgg gagccgccgg ccttttctgc cgcagctggt ccaaagcagc ctgtacggcc
      661 ggcacatcga tgcagtcgct ttccttttca gcgacgtcga tgcccaggcg cagcttggct
      721 tccttcttga tggccttata gaggcctttg gggaaatcgg ccagcgtcgt cgtcacgccc
      781 cgcgggaaga cggcttcccg ttccagcgta tagccgtcgg gataacggcg ggaacagcgg
      841 ggatgataca tgtcgacata caggcggtcg acgaacttgc cactgacatt ggtcaattca
      901 aagacgtcca ggttcccgac ctgaccggcc ttgtggaaaa agaccatgga acccgtccgg
      961 cgcaggcgca ggcgcgacaa atagacgaat tcaccgctcg gtccgttgac ggggatggga
     1021 ttggtcgcat catggccaaa acggccgtaa gctccgggaa ggacatcgca gtcttcccca
     1081 tgccgtacct tgtcggccag ctctcccgat aaagcctggt tctgcaattc cggtgaatta
     1141 aagatctgca aggcattgtc atagatctcc tcgctcttcg tccgctgatg ttctttaaaa
     1201 aaggcatcaa aaaatcccat agtcttcctc ctaatatata taatacatta ataatagcat
     1261 ctttaatgga tatcatccgc caaacactca acctgtcatc tggcagatat acttattata
     1321 aggcatttca cgccatgccg ctagtgccga gaaaagcgtc ttttactaag ttttaagttc
     1381 ttcctttatt taaacgtaaa ttttaatatt tttctgttat gaataaatat ctattttaca
     1441 gtgctttata cttcctgttt tttacctcat ctttcttctt tttaaaaaat cagctccccc
     1501 attttcttga ttttatatcg tcatttagcc atttattgcc tttaaatcat ttatcagccc
     1561 tgtcgttgca tttttcagcc attgatttta tttactcgta tgttattata tttatagata
     1621 aagagttctc ctacaggtag aacgaatatc tggtaatcat ttaaaattat taagtatcgt
     1681 catataataa ggaagaaaag aggtaagaac tatgaactta agtgccaatg caaaaatcat
     1741 catctccatg agtgctttac tttcatacag tttcatcgct catcttcttt aagagggata
     1801 ggaaaagggc ttgccgcgac gcggcaagcc cttttttagt ggctcgtggc taggggctag
     1861 gggttcgtag gggccgtccc aaagggcggc ccgcctagga aatgtgcgat ttcccactcc
     1921 gtttgctgag agaatttcca aaattcataa cgggctcccc acgtggcgcc cctacaaact
     1981 atgttgaaag agcaacctct cagtcagctt cgctgacagc tctcctatga ggagagccgg
     2041 gctacgcgat tttatatttc ttttccaggc ggtccaggag ttccatgtag ccttttacga
     2101 attccgggcc ttcttcttgt tccatgtctt ttacagtctg gtcgatttta gctttgatat
     2161 catcggacag gccgcggtcg gctaaggggt agacgacgtt gttttctttt tctacgtgct
     2221 gctgcaggcg gttggcatag gccatggctt cggtcaggac ctggagttta tattccgtct
     2281 tcggttcctg ggcgtagagt ttgagggctt cgtcgagggc ccggacatgg gcacggatga
     2341 ggtcgtgttc gacgagcatg ccgtgcttga tgatgatgga ggcgacttct tccaggtggt
     2401 cgaccatttc agggaaaagg acgagttctt cccggcggtg atggtggccg tcggcataac
     2461 ggcggatgaa atcgaggatt tcggcaaatt cctgctggtc gatttcagcc ccttccagaa
     2521 tagcgcaaca cgattctttg atgactttca gcatccgtga aatattggca tgttgtttaa
     2581 ccatgacttc cgtgcaatac ataaggctcc ctctctttcg tttaggcaga tttcttgacg
     2641 atttcatact ggccttcggc cgggctggtg aaggcgtaat ccgtagccga taacagaccg
     2701 tcgaccaagg cctggcgcag ggcgtagtag cggtcgccgt ggccctgcca gaattcgctg
     2761 tgcaggtcca tagtccggtc ccaggtgatg cggctgtcat cgctttcgcg gacgcggttg
     2821 acctggtcgc agggcatccc gtccaggagc aggtcgtcca ggcggtggaa ggcttccggg
     2881 gccgtagtgg cattgagatg ctggctggca ccgaagtcat aagcggcctg tttcagtgcc
     2941 gtcaggcggt cttcctggtc accggcaacg tcgagtacca aggcggcata acggccttcg
     3001 gcatcgtgga tgcgcgactg gagccagccg tggatgttcg attcgtcgat gacttcatcg
     3061 agtttgcggc ggtctttgct gccgtatttg tcgaccaaat cgctgtcgga ccagccctgg
     3121 ctggatgcgt agcggatgag ttcgtcgatc aggctttcct ggaactggat tttattgtat
     3181 aaccaaaaat ggatgggtcc taaaaatgcg ctcatgatat gtcctcctct tactgtttgt
     3241 tcgcttcaat ggcaacgttg acggctttag taacgatttc cgggtccagg ccgtggacaa
     3301 agcaggcatt ggccaggctt tcctgctgcg acgacgggca gcccaggcag tgcatcccct
     3361 gagaaataag ggtatcgacg acttccggat gaagacggat gatatcgccc ataatcatat
     3421 cagacgtaac tttttcattc attgcgaatt tattcaaaac agatttcaac atagtaccta
     3481 cctcctaagc aagtggatga attatatctt atctctaagt gaggttctct caacttctga
     3541 acgtagtata ccatggataa ataggacaat cggttgttat tacaacggtt tggggattta
     3601 gtggctcgtg gttcatggct agtaaaaaag cgctgtcttt gcgacagcgc ctttttaggt
     3661 ttgcagcttg cagtttgtag tgaatggttc actagccact aaccactctt attcttcctc
     3721 ttccggaagt tctgcgttgt ggtatacgtc ctggacgtcg tcgagttcgt cgagggcgtc
     3781 tacgaagcgt tccatttttt cagcgtcttc tcccgtgagt ttgatcgtgt tttccggaac
     3841 catggtgatc gatgcgtgtt ccgtttcgat gttgttgtca gccagggctt tttcgacagc
     3901 gtcgaaggct tccggcgtgg tgatgatttc gtagtcttcg tcggaaactt ttacgtcatc
     3961 agcaccggca tcgagggcga cttccatgat ctggtcttca tcggcctggt cgcggttgac
     4021 gacgaagatg cccgtgttgt ggaacatcca gctgacacag cccgtagcgc cgaggttgcc
     4081 gccggatttg gagaattcgt ggcggacagc agcggctgcg cggttgcggt tatccgtgag
     4141 gacgttgacc gtaacggcaa cgcctgccgg gccgtagcct tcgtatacga tttcttcgta
     4201 gttctggcct tctgtagcgc cagcgccttt ctggatagcc cgtttgatgt tatctttcgg
     4261 gatattgttt tcctttgctt tctgaagggc cagcttcaga cgcatgttgc cgaccgggtc
     4321 cggaccaccc atgcggacag caatcgtgat ttcacggccg attttggtcg tgatcttacc
     4381 gcggattgcg tcgtttttcc cttttttacg tttaatattc gcccatttag aatgtccaga
     4441 cattcaaatg cctcccttta tttccaccaa tcttcgccgg ccaggcgatc taagatgata
     4501 gctgctgccg aacggacaga taagtgatta tattccccgg ccccgtaaat cggttccagg
     4561 atgaaatcaa atttttccat cgtttctttc gtcatcccgt agcccgtgcc aaagagcagg
     4621 aggatcggcg tatcgtcttc ttcccgcttc tgccgcaact ccttatagga aatggtgttg
     4681 gcataggtcc gcgcgtccgt cgtgacgacg taagggcgct tcccttctgc ggcggtgacg
     4741 gcttcgacag ccgcttcgat ggacgggcgg atgtctacga tatcgaaagc ctgggtgcgg
     4801 tagccgttat agttgcggcc caccgggctt tcccagaaat ccatgatttt atgcaccata
     4861 tcgagctgcc ccggaatatg gtggatgatg aaatatttct tgacgccata agtcttggcc
     4921 gaccgcgaaa tgtcatgcag atcgtagttg gtaatagccg tggcaatgac gttgaagttc
     4981 ttatcatata tgggataatg aaccaatccg acatacaaag gggatttcat catttcttcg
     5041 cccctttcag tttagccagt atcttttggt cagctggcga caaatcgcaa tgctgcaaaa
     5101 ggtccggccg taattccaaa gtccgcgcca gcgacgcttc ccgacgccag gcatcgattt
     5161 tggcgtggtc gccgttccgc aggacctccg gtacggccat gccgcgaaag acggcaggct
     5221 tggtgtactg cggatactcc aggagcgacg aatagaaaga atcgtcagcg gccccggcag
     5281 ctgcgccgag gactccggga atcatgcggg ctacggcatc ggtaacggtc atagccggca
     5341 gttcaccgcc agtgaggacg tagtcgccaa cggaaatggt ctcatcggcc aaatcctgtt
     5401 cgacgcggta atcgacgcct tcgtaatggc cgcataataa aataagctgg tcgtatgtcg
     5461 ccagctcgcg tgccttggcc tgcgtaaagg tctgccctgc gggccccatg aagatgatgc
     5521 gccgccgcgg cacctgacgc cgcacggctt cgacagcggc atagaggggc gctgccttca
     5581 tgagcatgcc gcagccgccg ccatagatgg tatcatcgac catgtgatga cggtcgtacg
     5641 tgaaattacg gggattggtc acatccagat ccaacaggcc ggctgcccgt gcccgcccga
     5701 tgatgctgtg ttggaagaac gtttcgatga gctccggaaa gagtgagata atgtcgattc
     5761 tcattcgtcc gcccactccg gcggatcgac gacaatcttc ttggcacttt catcaatgga
     5821 cttgatgaca tcgggaatgg cggccaggag tgtttccttg ccgtcatcgg cagtgacgac
     5881 atagacgtcg ttgctgcccg tgcgcagtac gtctgtaagt tttcccagtt catggccctg
     5941 aaggtcgtat acggccgagc cgaggatgtc gtagatgtag tgctggcctt tctgcagggg
     6001 cactaaatct tcgcgcggca cttgcaggtc ttttttgacc aacagttccg ccgcattgcg
     6061 atcatcgacg ccgacaaatt tcatgaggac gacccgctta tgaagacgtg cggattctac
     6121 ttcatatttc ttgccgtcaa tgtagccgta tttcattttc aggaatcgtt tcggaaaatc
     6181 cgtatcggga taaatacgaa catcaccccg cacgccgtgc ggggcaacga tttgaccgat
     6241 tgtgaaaaga tctaattcag ccatcgtatt ctattggcgg atagaaacga tcttgccgtc
     6301 ttcaagcagg atttccgttc ccatcaattt ttctaaatca tcgccgacat tgacaacgac
     6361 agtctgttca agcgtgcctt gtctgatttc ggaaccgatt gtcaactgtt cagcttcttc
     6421 tctgtcgtgc tgtgccttag ccttagctgc ggcgagacgc tgtttctgtt cttctacttt
     6481 agcacgcaag gaaatcaacc cttgtgcatc gattttagct tgttcggtca tcatgcgttt
     6541 ggcctggaat tccaggttct gcatttcccg atcgatgtca ctcagggcct ggtcagcacc
     6601 tttgaggata cgatccttga gggcctgcgt aacctttgct ttgacagcga caggaacgcg
     6661 caatgtaatc tgatccatgg ttcctcctat acgatatcca ctgatacttt cttgttagcc
     6721 ttcactgccg cagccttgac aacaaggcgc aatgctttcg caattttacc ctgtttgcca
     6781 atgactttac ccatatcttc gggtgcgaca tgaagctgat agacttcgag gccttttttt
     6841 tgaatcttgg taactgtaac cgcatccggg ttcttgacga gggaccgggc gatacatgca
     6901 atcaattctt ccatagtatc cagcctcact tactttttgg atgcttcaaa ctgagccatg
     6961 atgccagctt ttttgaagat agaacggatc gtatctgtcg gctgtgcgcc tttgccgagc
     7021 catgctacag ctgcttcagc atcgacggtc agacgattgt ccatgtcttt ggacggatcg
     7081 tacatgccca acgtagcaac cgggcgaccg ttgttacgag catcgcggga atcagcgacg
     7141 acgacgcggt agatcggttt ctttttagca cctaaacgag ccaaacgaat tttaatcatt
     7201 gaaaatcacc tccttgatat agtatcttat ttccacaaaa aagggaaatc catttaaaaa
     7261 ggcagacgcg gcatgaaacc ccgtcgttta ttcttctgtg ctttcttggc ctgcttcatc
     7321 atcttctgca tttctgcgaa ctgcttgatg agacggttga catcctggac gcgcgtgccg
     7381 ctgcccttgg cgatacggcg cttgcggctg tccttgatga gcattttcgg attggcactc
     7441 gaccgttcct gggccgtcat ggatgtgatg atggcttcga ttcgtttgaa ttctttgccg
     7501 tccatgtcga ctttgtcgag ttctttctta tatttcccga tgcccgggat gaggctcagg
     7561 agactgccca tattgcccat tttcttgatc atatggagct gggacaggaa gtcatcgaag
     7621 gtgaaggtac cacttttcat ggtcttcttc atgttttcca tcgattcttc atcgaaagcc
     7681 gcctgggctt tttcgatgat cgtttcgacg tcgcctaagt cgaggatacg cgatgcgtag
     7741 cggttcggat ggaattcttc caagccgtcg agtttttcgc tgacgccgat gaacttaatc
     7801 ggtttgcccg tgacggcctt gatggacaag gcggcaccgc cgcgggcatc gccatcgagc
     7861 ttggtcatga tgataccgtc gatgccgagg gctttatcga aggccgatgc agccgtaacg
     7921 gcatcctgac cggtcatggc gtcgacgacg agcaggattt catggggatg aacttcattc
     7981 ttgatgttga tgagttcgtc catgagggtt tcattgatat gtaaacggcc ggccgtatcg
     8041 atgatgacga catcgcgtaa atggctggtc gcatagggaa tagcgtgctg agcaatggcg
     8101 acagggttct tgctgccctc ttcggtatag acaggcatgt ccagttcctg gccgaggacc
     8161 tgaagctgcg tgatagccgc tggacggtag acgtcggctg cgaccatgag cgggctcttg
     8221 ccctgggact tcagcttctt ggccagttta gcggctgtcg tcgtcttacc agccccctgg
     8281 aggccgacga gcatgatgat ggtcggtggc ttgggagaaa tggtgatccg gctctgtgtc
     8341 ccgccgagga gttccgtcag ttcatcgcgg acgatcttga tgaccgtctg tgacgcattg
     8401 aggctgccga agacttcttc gccgacggcc ttttctttga ctttcttgat gaaatctttg
     8461 gtgacggcaa agttgacgtc cgcttccaga agggcgcggc gaatctcttt cnnnnnnnnn
     8521 nctttcagcg cttcgctgac atcctcttca gaaagcttcc ctttgccgcg gagtttttta
     8581 aatgccgcct ggaatcgttc ggacagactt tcaaatggca taaggggcct ccttctataa
     8641 ctctatttca tgcaaaagct gtttcgctct ttcaagggtt tgcggttctt cccggtcgag
     8701 aagccgctct acttcatcga tcttatcccg gaccgcatcg tagcgggcca gcagatgaag
     8761 gcgctgttcg tagctttcca gcgaatggga agcgcgcttg agcatatcat aggcaccttg
     8821 gcgggaaatg tgcagttcct ccccgatttc gctcagggac aggtccatat cgaaatacat
     8881 ttccatacag cgccgttgtt tctccgtaag caaaggcccg tacaaatcga gaagccgtcc
     8941 cttgcggact acatcttcaa gcatggcttc accgcctttc aaacagcgta ctttggtatt
     9001 ataacggaaa gcagcctatc tgtcaagtat ttttacttgt caggggttcg taggggccgc
     9061 ccaaagggcg gcccgcaggt cgcaggtcgc aggccgtgcc tcgtggatgg ctttaaattt
     9121 agtcccgtct gctaaaaact acaaactgca aactaccaac tattacggta cgatggatac
     9181 gcggacgtcg tagaaggtgc tgccggcgcc ttcgtcggtc aggcgctggg acgtgaggac
     9241 gttgactgag gctccctgcc ctttgacccg ttcccgccac cagacgcctt cgctgacgac
     9301 ctggcccggc acagtccgtt tagagatgac gacggtgaaa tcggcctggc cccgttcatt
     9361 ggccaaggtc acggtctggc cgtcctgcag gccatagcgg gccgcatcgt cgggatggac
     9421 gaagaggtcc attgtatggc tggccgtcag ttcgtcccgt tcattgaagg acgaatcgag
     9481 gatgcgcatg tccggcccgt tgacgaaagc cagcgtgccg tcgtcttctg caaggggcag
     9541 atagtccggc agggccggcg tcagggccgc gttgtatatc tcgattttcc ccgacggcgt
     9601 ggcaaatcgc gtctggtaat cgtccggcat gggcagcggc gtcggcaggc cctgactaag
     9661 gcgctgctga tccacaggca ggggccattt cgtcgtcgac gcagccaggg cttcgacgag
     9721 gtcccgttct gagcaatcgt agaaatcatc gtctataccc atagcatggg ccaggtcaca
     9781 ggtgacgcgc cagttggagc ggctctggcc gacaggcggg atggccgcat agccgcactg
     9841 gatcgtgtag ttgccatagg aataataggc gtcgtcgtgt tccagcgacg tcgtcgccgg
     9901 caggacgata tcggcatagc gggccgtatc ggtcataaag cgttcgtgga cgacggtaaa
     9961 taaatcgtcc cgacagaggc ccttcaggac ctggttctgg tccggcgccg tgcaggccgg
    10021 attggacgaa tagacgaaga ggctgcggat gcggtcgtca cccgtcagtg cctggcccag
    10081 gcagcacatg ttgacgacgc gcttgccagg ctgtttccaa tcggaatggg tgacgatttc
    10141 tttatcatag gctttcgacg ccgacgccga agtcaagagg ccggctccct gcttccccca
    10201 ggcgccgacg acagccggca ggcaggtgat gaggcgcgtc gtcatggcgc cgttgcgata
    10261 gcgggactgg ccgctgccga ggcggatgaa cggcgcccgg gcccggccgt aggcgtgggc
    10321 caattcgacg atgacgtcgg ccggcacgcc ggtgatgccc tgcgtttttt ccggcgtata
    10381 cttggacagg acgttctttt tcaagtcctc ccagccgagg acgttccggt ccaggaaggc
    10441 ttcgtcagcc aggccgtcac ggtcgaggac atgcatcatg cccagggcca gggctccatc
    10501 cgtaccgggg cggacggcga tgaagtggtc ggccaggtcg gccgtcttgt tgcgataggc
    10561 atcgatacac cagacgacgg cccctttggc ccgggccgct tcgacgtcgt ggtggaagtg
    10621 gatattcgtg gccagcatat ggatgctcca gaggaagatg cagtcgctgt cctgggctgc
    10681 ctgcggcgcc gtcggcatgg tattgcccat gacgtccttg aagccccgcc ctttggccgg
    10741 agcgcagatg gtccggtcca gttccgaagc acccaaacga tagaacaggc cgtgcagggc
    10801 gtcgtgctgg acgatgccca tggtaccggc ataagaatag ggcaggatgg cttcggcacc
    10861 ggcttcggcg atgatggcct tccaggtccc ggctatttct gccagggcct ggtcccagga
    10921 aatgggcgcg aactggccgc ttcccttggg gccaatccgc ttcagcggcg tcgtcaggcg
    10981 gcgcggcgaa tggacggtcc gctcataatg ggccatcttc gggcacaacg tcccgcgcgt
    11041 aaagggatgg gacttgtcgc cggcaacgga tacgacgcgg ccgccgtcga ccgtcacgac
    11101 gagcccgcag gcatcggggc agtcatacgg acagacagat gtacaggttt tcatggtatc
    11161 tcctcttgtt t
//
LOCUS       DAWABZ010000051        11066 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_1625_length_11066_cov_3.059849, whole genome shotgun sequence.
ACCESSION   DAWABZ010000051 DAWABZ010000000
VERSION     DAWABZ010000051.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 11066)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11066)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11066
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1625_length_11066_cov_3.059849"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2411 a         3041 c         3204 g         2410 t
ORIGIN      
        1 tggatctaaa tggagatatc atgagggagc acggaacatc ccttttcaca cagtggcagg
       61 aaaatcttga tgttttttat cgtgaagcgg agaagattcc cgggctgcgg atgcttcagc
      121 cggccgatct gcaaagcggg agcatggatc gaaccaagat cgctctggac atgagtgctc
      181 tggggctttc gggagcacgg ctggaacagg aactgataaa gcgtgatatt ttctgtgagc
      241 tgacgacggg cagcatcctg atgtgcatga cgggaatcgg caatacgaag gcagactatg
      301 agaagctgct ggcagctttg caggagatcg cagagcagga aagccgaggt gaccggatgg
      361 tgcaggacgt gccaagagat gcggaaaaac cgaagcacac taaactctcg gaaaatccag
      421 caaaatcgaa aatcccgtgg aacaaaaaaa gacccctgca agatactgca ggggaaaaag
      481 agacagagga tatcagaaaa tgcagcggac gcatatgcgg aacctccatt ataccgtatc
      541 ctcccgggat acctctcata tgtccgggag aagtcctgga cgatgaggat atccaatatg
      601 tgcttcacct cagaaagaga ggagagaaag tcatcgggat cgatgaacaa aacaggatca
      661 cggtcatttc aaatcgccgt tgatcccaat ggtaacgacc ttcagcggaa tatcctttcc
      721 gctggcgtcg actgcctgct ttacagcata ttccagaccg ccgcagcacg ggacttccat
      781 gcggaccagg gtgacgtcgt ggatgtcgtt ctgacggagg atttctgtca atttttcggc
      841 atagtcgatg tcgtcgagtt tagggcagcc gatgagggtg atgcggccct tgatgaaatc
      901 ttcgtggaag cgggcgtagg cataagccgt acagtcggcc gcgatgagca ggctggcacc
      961 gttgaaataa ggcgtatggg gtggtacgag cttgagctgg accggccact ggctcaggcg
     1021 ggagatggac ggcccggcag gaacgtcgtc agcccttttc tgcgtttcgc cgaggctgcg
     1081 ggcctggctg ccgggacagc ggccggtgta ggtccgtccc tgggcggcca tgtgctgttt
     1141 cacggcttct tcatcatagg cagcggcttc gcgttcgaca aaggtgatgg catccgtcgg
     1201 gcaatggggc aggcagtcgc ccaggccgtc gcagtagtca tcgcgcatga ggcgggcctt
     1261 gccgtcgacc atggcgatag ccccttcgtg acaggccgtg gcgcaggcgc cgcagccatt
     1321 gcattttttc gaatcaatct gtataatttt acgtatcata atttcctcca tcgtggtata
     1381 tgcatcaaac tggtttggga cctttatggt ctaagtatag tcgatggagc aaaaataagc
     1441 tgttgttttt ccaactatca tctttttttg gaggtctttc catggaacaa tatctttccc
     1501 tcattgaaac gtcgccgctc ttccacggcg tcgccgaagc cgatgtcctg ccgctcttgc
     1561 agcgcttgaa ggtacgcaag aaaaaatatg agaaagggga attcctcttt tattccggcg
     1621 atgccgtccc ctatatcgga ctggtcctgg aaggggcggt ccacatcatc caggaagatt
     1681 actgggggaa ccgcaatatc ctgtcccaga tcccggcagg cttctttttc ggcgaagcct
     1741 ttgcctgtct gcctgacgcg ccggcgacgg tcgacgtcgt cgccgcctcg gatgcggtca
     1801 tcatgcaggt ctatgtcggc aacatcctcc atgccggtca ggtcctgacg ccggaccagg
     1861 ctcgttttac aggcaatctc ctggctctga tggccgaaaa gaaccgcctc cttacggaaa
     1921 agatacgcta cctgacccag cgctcgacgc gccagaaaat catgctctac ctgtccgaca
     1981 tggcccagaa aaaagggaag gccaccttcg tcctgccttt caaccgccag cagatggccg
     2041 acttcctctc cgtcgaccgc agcgccttat cggccgaact gagcaagatg aaaaaagaag
     2101 gcctcatcga ttaccacaag gacaaattca ccttgcggca ggaacattaa ggaaatcggc
     2161 gggccgccag ctaagctgtc cattctggca gctaccctat cggtacctgg tgggccagaa
     2221 aggctttcag gacggctctt acaaaccacg agccacgagc cactgctaaa aaaggcgctg
     2281 tcttcatgcg acagcgcctt ttttactcgc ttttaatgca gatgggggag gatgtggccc
     2341 attttattct ttttcgtttc caagtagaat tcgtcgaatt tctggggttt catttcgatg
     2401 gggacgcgtt ctacgatttc gacgccgaag ccttcgaggc cgtagacttt ttccgggttg
     2461 ttcgtgagca ggcggatttc tttagcgccc atgtcctgga gaatctgggc accgatccag
     2521 tattcgcgaa ggtccggagc gaaaccgagt ttttcgttgg cttcgactgt atcgtagccc
     2581 tgttcctgga ggacataggc tttgagttta ttgatgaggc cgatgccacg gccttcctgg
     2641 cgcatgtaga ggagaatgcc ccggccttcg gcgttgatct gtgtcagggc agcggccagc
     2701 tgttcaccgc agtcacagcg gcgggagccg aagacgtcgc ctgtaaggca ttcagaatgg
     2761 acgcggcaca agaggttctt gccgtcgccg atttcgcctt tgaccagagc gacgtgatgt
     2821 tcgcccgtca ggtcgttgat gtagccgtag atcttgaaat cgccgtattt cgtcggcatc
     2881 ttggcgacgg cttcgcggat gacgtgtttg tcatggcggc ggcagtagtt ctggagatcg
     2941 tggatggtga tgaatttcag gccgtattcg tcggcgattt tccagaggtc cggcgtgcgc
     3001 atcatcgtgc cgtcgtctgc catgatttcg cagcacaggc cgacctgttt gagtccggcc
     3061 aggcgcatga ggtcgaccgt ggcttccgtg tggccgttgc gggtcaggac gccgccgcgt
     3121 ttggcgacga gggggaagtt gtggccagga cggcggaagt cttcgggacg ggccgattct
     3181 tcagcgcatt tgcgggccgt cagggcgcgt tcgacagccg agatgcccgt cgtcgtgtcg
     3241 acgtggtcga tggaaacagt gaaggccgtt tcgtggttat ctgtattttc gctgaccatc
     3301 tggggcaggc cgagcttatg ggcatattcg atgctcatgg gcatgcagat caggcccttg
     3361 ccgtggctgg ccatgaagtt catgttttct gtcgtgcagt actgggcggc gcagatgaag
     3421 tcgccttcgt tttcgcggtc cgggtcatcg gtgacgatga tgacgtggcc ggcctgtaat
     3481 tcttccaatg cttcttcgat agtgttgaat ttacgttttt ctgtagtcaa aataaagacc
     3541 tccttaaagt ctgtattgtc catagttggt ggtactcaaa aagccctggc gatgaggcac
     3601 agggctttga catgcaagga aaggcacgct gcagaaacag cacgccacat cttcttccat
     3661 ccagactata ctgtcggttt tggaatcgca ccaaatcacg agccaatggc tcctacgccg
     3721 tagcgttcgc ggactatacc gccgatcggg aatttcaccc tgccctgaag atatacgttt
     3781 tattcagttc tgttatgtat tatacacggt aactttcatt tttgcaagtt agtcgacgtc
     3841 gaagattttc cccgggttga ggatgtcgtt cgggtcgagg gctttcttga tggccttcat
     3901 catttccagt tcgccgggat cggtgaattc ttccatcaaa tgcttgcgct tgtagccgat
     3961 gccgtgttcg ccggacaggc ggccgccgtg ggcataaatg aatttgtaca gttcctgctg
     4021 gttggccttg acccgctggt cccagtcttc ataggtggca tcttcattct tgaggatgtt
     4081 caagtgaatg ttgccgtcgc cggcgtggct ggcgatacgt gtgaccaggt tgtatttctt
     4141 ggccaggcgc aggatttcgt cgaggagggc cgcttcctgg tcgacaggga cgacgacgtc
     4201 ttctttgcag acgatgaggc tttcggcgcg gacggcttcg gcaaaggcct tgcgggcctg
     4261 ccagatctta ttgtggtcag cgacgaggac tgacgacgcg ccgttttccg tgcagacttc
     4321 gtcgaggatg acggccttgt cgtccaggtc gtcttcgctg gtcccttcga cttcgacgat
     4381 gacgtagttg ccgttgtcgc tgccctggag attttcattg aggtattttt cgacggactt
     4441 gatggtgatg ttgtccatga attcgacgca cgtcggcgtg atgccggcct tgatgatttt
     4501 ggtgacgacg cccatggcct ggcggcggtc gtcgaagatg gccagcaggt cgagggcgta
     4561 cttcggcttg ggcaggagct tcagcgtcgc cttggtgatg atgcctaggg tcccttcgga
     4621 gccgatgacc agctggtcca gggaatagcc tgtcgactgt ttctgcaggc gcgcgccgag
     4681 gttgacgatg cgccctgtcg ggttgacgac ttccacgctg tagacctggt ggcgcgtcgt
     4741 cccgtatttg acggcccggt tgccgccggc gttggtggcg atgttgccgc cgatgaagca
     4801 cgagtcgccg ctgcacggat cgccggcgta gaagagacct ttcttttcgg ccgcttcctg
     4861 caggtccgac gtgcgtacac ccggttcgac gacggcatag aggccgtctt cattgatttc
     4921 cagtatcttg ttcatctttt ccagcgacaa gacgatgccg ccgtattccg ggacggcgcc
     4981 gcaggccaga cccgtgccgg ccccgcgggg gacgacaggg aagtggcagg cattggccag
     5041 cttgatgatg gcgctgacct gctgggctgt ttcggcaaag acgacgactt cgggcatgtg
     5101 atggtaagcc gggtcggtca cttcgtcatg ggaataaggc tgcatggcgt cggcgtcggt
     5161 gacgacgtct ttcgggccga cgatggcctt taatttttcc aggatttctg tcgttatttt
     5221 ttcatagtgc atacgtgttc cccctcggat aagatacaaa atgtcgtgcc tctattatat
     5281 ataactgccg cccattttaa aaggaatttt acaaaaaaac tttatcttgc cagcaaagca
     5341 cggtatacta tagggtaaga ctttttcagt ctcttgttct tagaaagggt gattgggtgg
     5401 aaaccaagca gtatcgccag cctcggcgcc gaaagaagcg ccgcaaggtc ccccggaagc
     5461 gccgtacagg gctgctcatc cggctccggc agatgagctg gtataatctt attctcgtgg
     5521 ccatcatcgt cgccggcttc tatctcatcg gcgtccgcct ctacaggctg tgggaaatcc
     5581 atgacgacat gaaccggacc ctgcagcagg aacagacctt gcgggacgaa aatcttcgcc
     5641 ttcgcgaacg gcgtgacaag ctgaatgatc ccgatgaaat cgcccgggaa gcgcgcgaac
     5701 agttcggcct ggccaagccc gatgaaattc cctataagcc gtgattccct atattttgta
     5761 ttttgatgat tgaatttttt ggcatttatg agtataatgg ctttatacgt ttagcagtaa
     5821 tgctgatatt acaaggagga aatcaaaaca gcatgacaat cgaagtaggc agtgtgatgg
     5881 aaggtaccgt tacaggaatc acaaaatttg gcgcattcgt agagcttccg gataagaagg
     5941 taggtcttgt acatatttct gaagtcgcta atgagtatgt caaagatgta aatgattttt
     6001 tgaaagttcg agacaaggtc aacgttaaag tgttgtctgt agacgataag ggaaaaatcg
     6061 ccctgtctat caaacagacc cagccggcgc cggaaaagaa agaattccgt ccgaaacgcg
     6121 attttcggca gaaacatgaa tttcgccagc ataacggctt tgattcgcgc cgttcgtcgg
     6181 gctcgctgtc ttttgaagac cggctcagca aattcctgaa ggaaagcgat gaacgcctga
     6241 tggatttgaa gcgcaatacg gaatcgaaac gcggcggccg cggtgcgcgc catgctgatt
     6301 gagtgcgtat tgaatagcgg aaggtaaaaa agaagggcgc tgttgcacaa agacagcgcc
     6361 tttttacgtt tacaggaagg agcagatatg gagtatcaga agtatgcagt catcgacatc
     6421 ggctccaact ccctgcgact catgacgggc tggcaggaag tcccgggcac gtggattttt
     6481 tcgccgaaag aactggcgac gacgcggctg gggaaagata tcgatgaaac acaccatttg
     6541 tcaccggcag ggatggaagc gtcctttgcg gccatggaag gctggcaaga acagctgaca
     6601 ggcgtaccgg tctgtgccgt cgccaccagt gccgtccgcg aagctatcga cggccaggcc
     6661 ttcctggcag aagtgcgggc ccgtttcggc tggcactgcc gggccatttc gggcctggaa
     6721 gaagcgtcgc tcagcttcac tggagctacg gcctcgctca gccccgatgt gacggcagcc
     6781 gtcatcgaca tcggcggcgg cagcagtgaa gtcgccgtcg gccgcggcgg tgacctgtgc
     6841 tggtcccaca gctaccccgt gggggctgtg cgctttacgc ggggcgaagt catgagccat
     6901 caggccgtcg cccatctgga aaaccgctgc tatcaggaat ggctgcccat gcaggtccag
     6961 ccggacctgc tcatcggcgt cggcgggacg ctgacgacgc tggcggccat ggacttgcag
     7021 ctggccgtct acgatgcaga gaaagtcgaa ggccatcgcg tttcctatga tgccctgtgc
     7081 ggccatatcg aacgcctccg ggccatgacg ccggaagaac ggcgccacgt accgggcctg
     7141 cagcccaagc gcagcgacat catcgtcgcc ggcctgctca ttgtcaagtc ctttctccag
     7201 cgctatcaca tcccggccat caccgtcagc gaacgggatc tcatggaagg cgtctttttc
     7261 cgccattcct tccatgatgc ggcctggaac tcccgggacc gccagtcgtg aaccttgtcg
     7321 accgcgtaga cgcttacggc aggcagcacg gcctgtggca gccggggagg gccctggtcg
     7381 tcgcctgctc cggcgggccc gattcgctgg ctctgctgga tatactgagc cgcctggccc
     7441 cgttatatag gctgaccttg acggcctgct acgtccacca cggcatccgc cgggcggccg
     7501 atgaagaagt cagcctggta cggcgcgaag ccgtgcgccg ccattgcggt tttgcctggc
     7561 agtacgtcgg cgtgccggtc ctggcccggc gggaaaaggc gtcggaagaa gccgtaggcc
     7621 ggcgcgaacg ctaccgcatc ctgcgggaaa cggcccgggc ctgcggggcc gaacgcattg
     7681 ccgtggccca tcacgccgat gatcaggccg aaacggtatt gctccatctc ctgcgcggca
     7741 gcggcctcga cggcctctgc ggcatggcgc ccaggcgggg cgacatcatc cggccgctgc
     7801 ttatggtgac caaggcggag ctggcagcct atgccgcggc ccgggacctt ccggtctgtc
     7861 acgacgaaac caatgacagc cggcgctaca gccgcaaccg gatacgcctg gacctgctgc
     7921 cgcagctgca ggcttataac ccggccatta cggcggacct gaaccgcctg gccgatatcg
     7981 tcagggccga cgaagccttt ctggacgacg ctgccgaaac actctatggg cagctcgtcc
     8041 ttcccgaaga cgtaccggct ttagacaaga agcgcttgct ggcccagccg ctggctatgc
     8101 agcggcgcct catccgccgc ttgtggcagg aggcgacggg ctctcgtcag gacctgcctt
     8161 ttcattatgt tgaaacgata cgggacctgg ccgccaaagg agctggaaaa cagttccagt
     8221 gcggccgggc ctgtgcttat acgacccgga cagccctctg cctgggtcct gccgttcccc
     8281 gcaggggacg gcaggggtaa ttcacttttt cacggaggaa aacaagatgc atcaggatat
     8341 caaagaaatt ttatttacag aagaacagat tgctgcccgc gtccgcgaac tgggtgcgca
     8401 gattactaaa gattatgccg gcaagaccat cctcatgtgc ggcatcctca aaggggccgt
     8461 cacctttttc accgacctgg cccgctgcat cgacggcccg gtctacttcg atttcatgag
     8521 ctgctccagc tacggttcgt cgacgacgtc gtcaggtgtc ctcaagatcc gcaaggacct
     8581 agataacgac gtcaaggata aagatgtcct catcgtcgaa gacatcatcg acacgggcgt
     8641 caccttgagc catctcgtcc ccatgctcca ggaacggggt gcccgctcgg tcaaattggc
     8701 acccctcctc agcaagccgg cccgccgcca ggtcgaagtc cctgtcgatt acaacggctt
     8761 tgaaatcccc gatgccttcg tcgtcggcta tggcctcgac tacgacagcc gctaccgcaa
     8821 cctgccgtac attggcattt tgaaagaaga agtatattcc aagtagttgt tactttagaa
     8881 gaagtgtggt aatataagac tgtattggtt ggaataaaaa agcattatca taaactaagt
     8941 gaaaggagcc atattttgag taaatttgtg cggaatgtaa gtttatactt actcatcatc
     9001 atcgtcgccg tatcgatcat agatgccttt accagcagta agacagataa atcggaaatt
     9061 tcttacacga atttcatgag tcaggtgcag cagaaaaaag tcgatgccgt ccagatcaca
     9121 gccgaccacg ctatcgtcgg acagctgaaa gacggaacct cttttacttc ttatgccccg
     9181 accgattcgg cactcttgcc ggcactgcgc gatgctgatg tcaacatcat cgccaaaccg
     9241 cccgaacagc cttcgtggtg gatgaacatg ctgacagcag tcctgccaat cctggtcctc
     9301 attgccatct ggttcttcat catgcagcag acccaaggcg gcggcggccg ggtcatgaac
     9361 tttggcaaga gccatgccaa gatgcacggg gaaggtaaga tcaaggttac ctttaaagac
     9421 gttgctggtg aagacgaagc gaaggaggaa ttggcggaaa tcgtcgaatt cctgcgcaac
     9481 cccagcaagt ataatgccat tggcgccaag atacccaaag gggtgctcct cttcggccct
     9541 ccggggaccg gcaagaccct cctggctcgt gccgtagccg gcgaagccgg tgtccccttc
     9601 ttcagcatca gcggttccga cttcgtcgaa atgttcgtcg gcgtcggtgc ctcccgtgtc
     9661 cgtgatttgt tcacacaggc caagaagaac gcgccctgca tcgtcttcat cgacgaaatc
     9721 gatgccgtcg gccgccagcg cggtgctggc cttggcggcg gtcatgacga acgggaacag
     9781 acattgaacc agctcctggt cgaaatggat ggcttcggtt ccaacgaagg catcatcacc
     9841 atcgctgcga ccaaccgtcc ggatatcctc gacccggcgc ttctccgtcc gggccgtttc
     9901 gaccggcaga tcaccgtcga ccggccggac ctgcgcggcc gccgggctat cctcgacgtc
     9961 cacgccaaag gcaagcccct cggcaaggat gtcgacctcg acgtcatcgc caagaagacg
    10021 ccgggcttca ccggtgccga cttgggcaac ctgctcaatg aagcagccct cctggctgcc
    10081 cgggctaata agaaagtcat caacatggct gaactggaag aagccagcga aaaagtctgc
    10141 ttcggtccgg aacgccgcag ccatgtcatc agcgataagg aaaagcgcct gacggcagtc
    10201 cacgaatcgg gccacgccct catcgcctat ctcctgcctg atgccgaccc ggtccacaag
    10261 gtcaccatca tcccccgcgg ccgggctggc ggctatacca tgatgctgcc ggaagaagac
    10321 cggtcgtatg aaacgaagtc gtactacctg gcccagatcc gcgtcgccct cggtggccgg
    10381 gctgctgaag aaatcgtttt caacgaaatc agcagcggcg cttccggtga ccttcagagc
    10441 gtcacccgta tcgcccgcca gatgatcacc cgcctgggca tgagcccgaa actgggcccc
    10501 atggtctttg gcgaacagca ggaccaggtc ttcctcggca agagcctggg tcacgaacgc
    10561 aactacagcg aaagcgtagc cgaactcatc gacaaggaaa tgcacgacac cgtatcggaa
    10621 gcctatgccg acgtgctcca catgttgcgg gaacacctgg atacgctgaa aaatatggcc
    10681 aatgccctga tggaagtcga aacgatcaat catcagcaag tcgataacct ggtcaaatac
    10741 ggcaccttgg aagggccgca gggcgttaaa aaagaagctg ctgaaacacc ggcagaaacg
    10801 gcagaagccg aaacaccgga cacagcagcc gtttccgaag ccgctccgac agtcaaggaa
    10861 gaagcacctg caccggcagc tcccaaagcc gaacagacag aagtacagat cagcccctgg
    10921 ggaaaccctg tccccaaagg cggcctgacc acaggcctga ccgataagga cgaagaagaa
    10981 aaaaaggctg acgaagaaaa atagagagtg aaaaaaggcg ctgtcgcatg aagacagcgc
    11041 ctttttgagt attaagtttg cagttt
//
LOCUS       DAWABZ010000052        10648 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_1708_length_10648_cov_3.080619, whole genome shotgun sequence.
ACCESSION   DAWABZ010000052 DAWABZ010000000
VERSION     DAWABZ010000052.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 10648)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10648)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10648
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1708_length_10648_cov_3.080619"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2073 a         3309 c         2976 g         2290 t
ORIGIN      
        1 caaagtcacc ctgcccggcg tgggtgaggt ctattacgtc tttatcgccg attacgaccg
       61 caaatacctg accagcgaca tcgacaacat gaccggcctg cccgggaacc ggcgcttcct
      121 ccagtatgtc cgggacctca tggaatccgg tcagttcctg tcgggatact gcctggtcta
      181 tttcaacgtc atcaacttca agctcgtcaa tatccgctat ggcgtcgccg tcggcgatga
      241 ctgcctgaaa ggcctggcca ccatgattcg ggaaaccttc ccgcagggct tcttgtcgcg
      301 tcgccgcaat gaccacttcc agctgctcac gccggaaggg tctgtgcggg aaaagctgga
      361 aacacttcat gaatccatct gccaggtcta tcgcaagtac cagctggaaa tcaaggccgg
      421 cgtctaccat tttcctgaaa atcaggtcgt cgacccggct ctggccgtgg attatgccaa
      481 aatcgcctgc cagcacatcc gcgatgaagt gggcgtctcc gtcttggaat atacccagga
      541 cctgcagaag ctgattacgg tcaacaacta cattgctgga cactgtgatg acgctattga
      601 gcacggctgg atccaggtct attatcagcc tgtcatccgc accctgaccg gtgccgtctg
      661 cggcatggaa gccctggccc gctggaatga ccccgtcgtc ggcttcctgt cgccggcccg
      721 cttcattccc atcctggaaa agaaccacga catctataaa ctcgactgct acatcctgcg
      781 ccagacctgc caggccctgc gccgccgcct ggatcagcag cttcccgtcg tcccggtatc
      841 cttcaatttg tcgcgtctgg actttttcct ctgcgacatc ttccaggtcg tcgaagatac
      901 ggtccggaca tatggcctgc cgcgggacct catccatctg gaaatcacgg agaccatgct
      961 ggtccacgat aaagaagtca tcaaagaggc cctgggacgc ttcctccagg ctggctacca
     1021 aattctcatg gacgattttg gcagcggcta ttcgtcgctc aacgtcctca aggattatga
     1081 cttcaatatc atcaagctgg atacgcattt cctctcgtcg tcgacggaca aggcccgttc
     1141 catcatcggc aataccgtcg acatgatcaa ggaaatcggc agccagagcc tggccgaagg
     1201 cgtagagacg cagaaacacg tcgatttcct ggcctctgta ggctgcgata agctccaggg
     1261 ctatttcttc agtaagccca tgccctggaa cgatttattt tcctatctgg aaggccagta
     1321 ccggtctttc gaagatgcgg ccagccgagc ttattacgag aaaaccagcg ccgccatccg
     1381 ccggacccat cagtccctgg ccctggtcga atacgacggc caagcattcc atcacgtcta
     1441 tctcaatcag gcctatcggg atcagctggc cagtttcggc cgcattacgg ccctcagtgc
     1501 cgaactgcgc atcaaccggc ctggaacgcg gctttatgcc catttccggg acctcatgga
     1561 aggcctcaag cagagcggtc atttggaaac gatcttccag tcggaaaagg gctactacgt
     1621 ccagctcacg gcggaactcg ttgcctccat gccgtcgtgc tggatgctga ccgtgtccct
     1681 gcacaacctg accctggacg aacacatcca ggagcaggag gcgctgaacg tccacattca
     1741 tgagctctac cgcctgtacg atgtcatcta cctcatcgac ttggacaaat cagccgggat
     1801 ccccttgttc tcgcgccatg ccgtagccgg ggaactcatg gagaaatgcc tcttcatgcg
     1861 ccagttcctt catcactatg cccggagcta tatccatccc gatgactgcc agcgcttcct
     1921 ggatttcacc gattatacgg cgctcaaaag gaaattatcc caaggggacc agttggattg
     1981 cttccgcttc cgccagaaag gagcggatgc aggctatacg tggaaagagg tcacgtacat
     2041 cgccgttccc cggagcggtc ggaaattggc cctggcctgt gtgcggccgg cgctctgctg
     2101 cccggcctgt cagctgcata aaggagggac cctttcatga tgcaaactat acggtcccgc
     2161 tttttgaccg tcatctgcgg cgtctccctg acgctgaccc tggtcttcgg cagcctctgc
     2221 gtcttcctcg tcgaccgcag cgagacggaa atcgccgcca cgaccctcac tggccgggcc
     2281 atccacgcca gtacgacact gaatccaatt ttcatgcagt ccgaagacat cgtccactat
     2341 atcggccata ccatcgagca tgaagtcaaa aatccccagg acttgcgtaa taaagccaac
     2401 cgcgaccggc tggaagccat gattagccgg tctttttata atgctgtatc cggcatcgac
     2461 ggcgtccagg gctattacct ccattacaat gaagacctgg ccggcgggcc ggacggcttc
     2521 tggtataccc gcaaagatgc gcgtcatgat tttgtgtccc agcccattac ctccgtcgag
     2581 gcgtacgggg aagccgacgc gggccggacg aactggtatt atcagccctt gcgtgagaag
     2641 agggccttgt ggacgacacc ttacgtcaat gacaataatg gtgtccgcat gatcagctac
     2701 gtcatgcccg tctacgtcca aggccagttc gtcgccgtcg tcggcgtcga tatcgacttc
     2761 aacatgctct gcgacctcat cggcagcatg aaggtctatg acagcggata cggcttcctc
     2821 ttcaaggact ggcagacttt ttatttccat cccgatggca gggaggtggc ctacgagttc
     2881 aagccgcgct tcgatatcgt cagccacgga gagctgctgg agcagtcagc gacggatacc
     2941 catttgattc attattacta tcagggcatt cccatggcca tgggctttgc gaccttgaag
     3001 aatggcatga aactgggcgt gacggcaccg gaacgggaaa tctatgccgg ccgccagctc
     3061 atgaccttcg ggatcctggc tgtgaccgtc gtcatcggcc tgttgtcgac gctcctggct
     3121 atccgggcgg cgaaccgtat cgtccggccc ctgcatgaca tcagtgaagc cgcccgccgc
     3181 ctgggccgcg gcgaatatga ccagcctctg gacagtacag ctcaagatga aatcgggcgg
     3241 cttgcaggcc atatgaacga tacgatggaa cggatgcgca aatacgtcgg cgccatgcag
     3301 aaacaggcct atcaggatga actgacggcc gtcaagaacg tcgccgccta caacaggaag
     3361 gtctacgacc tcaaccagaa aatcgccgcc ggccaggctg cctttgccgt ggtcatggtc
     3421 gacttgaacc agctcaagga cgtcaacgac cgttttggcc acgaaaaggg gaacatcgcc
     3481 ttacagcgcc tgtgccaggc cgtctgccgc atctataagc acagcccggt ctaccgcatc
     3541 ggcggcgacg aattcgtcgt catcctcgaa ggcgaggact atgctcatcg ggacgcctta
     3601 ttcgagaaag tccgtgcctt tgaacacatc cgcgacctga gtgccgctca gccgtggacg
     3661 cagctcgccg cttccgtagg catggccgtc tacgacaaga cacgcgacac ggcctaccgg
     3721 gccgtcttca atcgggccga caccaagatg tacgaacaga agcagcacct ggcgccgcgg
     3781 gcctgagggt gagtgttggc tggctcgggg caaaggggag agggctgcca gatcatcgcc
     3841 ttggcggcga aaaggaaaga tgtacaaaaa ggacaaaatg tgatactatt tgtaggggct
     3901 ggaacatggt ggtttgtgcc gtcatgtgga gccctttgtc ttcaaaagtt gtagtatagc
     3961 gtttgtagtt tgtagaggat gggttttaat ttttagtcat tcactacaaa ctaaaaactg
     4021 caaacttaaa gtataaaaag aggctgtctt cgtgtggcag cctcctttct atttcgactt
     4081 acaaggaggt ctttcctttg cgtatcgttg tcgtaggcgc cggcaagctg gggtacagta
     4141 ttgacgagct cttatcgaat gaacaattcg acgtcgtcgt cgtcgatcat aatgaagaac
     4201 ggctggaagt cgtcaagaat aatctcgatg tattgaccgt cctggccaac ggggccagcc
     4261 cgattaccat gaacgatccc gatatccgcg gtgccgatat cctcgtcgcc tgtacggctg
     4321 tcgatgaagt caatatggtc gcctgtatca tggccaagaa acacggcatc aagtacacgg
     4381 cggcccgcat ccgggacatg cagttcctgt cggaagccaa ggattatctg aagcagaatt
     4441 tcgacatcga cctcatgctc aatccggaaa tgatcacggc ccgggaaatc aaccgcatcc
     4501 tcatgacgcc ggccgccctc aacgtcgaag acttcgccaa cggcaaggtc cggctctttg
     4561 aaaccaaggt ccgccgccat tcgccgctca tcaacatccc ctttaaggat atgaacatgc
     4621 cgccgtccat cctggccggc atgattttcc gcgatcaccg catgatcatc cctcatggtg
     4681 atgactgcct gttcccgcac gacaatgcgt atttcatcgg cgaccccaag gccatcgaga
     4741 aattcagtga gaacttcgtc cccagcgaca cgaagatggt cgaacgggtc atcatcatcg
     4801 gcgccggccg gtccggccgt tttttggccc ccatgctcga caaggaaggc atcaaggtca
     4861 agattttcga taaagaccgc gaacgctgcc gccaggtggc agaactcctg gacaacggcc
     4921 tggccatttg cggcgacggc acggacatcg acctcctgga acaggaaggc attgccgatg
     4981 ccgacgtcgt catctgcctg accgatgacg accgcctgaa cctcatgctg gccctcatcg
     5041 ccaagcactt gggcgccaag aagaccatcg tccgcgtgtc gcgcatcgaa tacggcgacc
     5101 tcatggaaaa ggtcggcgtc gatatcgtct tgtcgacacg cctcttgtcg gccagcgaag
     5161 tcctggcctt tgcccgccgc ggcggtgtcg tctccgtgtc cctcctggaa ggggccaagg
     5221 ctgaagccgt ggaagtcatc gtccaggacg gggctcccgt agccggaaag cgcctcatgg
     5281 atgtccgcct gcccaaggaa tgtctggtct gcgcctatgt ccgtgacggc gaagcctata
     5341 tcccgaacgg ccagtccgtc ctcatggccg gcgaccggat catcctcttc atccagacgg
     5401 aatattccaa gaaggtcatg aagtacttca aaggcaggga atagaccatg aaccgcagta
     5461 tcgtctttta tctcgtcagc cgcctggccc tgatcaacgg tctggcgctg ctcatccctc
     5521 tgctgtccgc cttgtggtgg ggtgaagcgg gcctggtcta ttttgccccg gccctggccg
     5581 ccgccttgct tgcagcggcg ttctgccgtt accgcggccg ccatcacaaa cgccacctgg
     5641 gaccggggga gggggcctgg tacatggtat cggcctggtt cctcctgggc cttttgggta
     5701 tgattcccta cgtcatggcc ggcactttcg catcgcctgt cgacgccttc tttgaaagcg
     5761 tctcggcttt tacgacgact gggacgtcgt gcctggctga cggcggggcc ggcctgccgc
     5821 agtcgctctt gttgtggcac agcctcatgg aatggctcgg cggcctcaac ttcatcctcc
     5881 tcctggtatc ggtcatgccc caggtcagcg gctgtttcgg cctgaccttg tccgtccatc
     5941 agagcgtatc cttcagcccc atggtggccc gcatgcagga agcggcccgc caggccggtc
     6001 tcatctacct ggccattacg gccttttcca tggtcctcta cgtcctggcc ggcctgcctt
     6061 tgggcatggc cgtcaatcag ggcctcatga ccatgtccac cagcggcggc gattcgctct
     6121 tcgatttttc tcaatatgac agcctggccc tggaagcggc cggagctgta tccatgctcc
     6181 tggccagcgg caatttcctc ctctactgga agagctggga acggcgcgac ttcaagagcc
     6241 ttttctgcga tgccgaatgg cggacctttt tgaccgtcgt cgtcgtcacg ggctgcctcg
     6301 tatccttcca tttgtggcgt ttcggcgtct acgacggctg ggataacctg cgctacggct
     6361 ttttccaggt cctgtccttc atcagcacca gcggtttttc gtcagccgcc tacagcacct
     6421 ggccggaatt cgacaagtac gtcctctttg ccctggcctt tgtcggcggc tgtatcggct
     6481 cggctacagg cggtctcaag gtcatgcgct tcatggtcct tttcaagatg gctgcccagg
     6541 aaatgcgccg gaccctacat ccgcacatgg tcatttcttt gaaaatcgac ggcgtccctg
     6601 tcgatatgaa gatcatcagc cgtgtcctca gttatttctt cctcttcatg gcgacgttct
     6661 tcgtctccat gctgatcatt tccctgtccg gcgtcacgcc catgcaggcc atggggatgg
     6721 ccgtaggctg cctgtccagt gtcgggtcga cgatggacct cttcggcgta gccgacgttt
     6781 ccgtcctgcc ggcatggact aagctgtatt gcagtttcct catgatttta ggacgcgtcg
     6841 aaatcttttc cttcctcgtc gtcatccaga ccgcattcgg ttatctgcgg cggcgctggt
     6901 aggaggtaca ccatgaatat acgaattgtt ttatttgtct tggggaaatt ggctcaggcc
     6961 tgcgggctgg ccttgatgat tccctttgtc gtcgcccttt tctatgggga agccagtatc
     7021 gccgcgttcc tcgtggccat tgtcatcagc ctcctcatag ggcgcctgct catgtggcag
     7081 ggccatcggc cgacggacag cctgactgtc cgcgaaggcg tggccattac ggctctgggc
     7141 tggatcatgg tcactttcct gggcatggtt ccctatgctg ccggcggcta tttgtccgtc
     7201 ctggacggtg tctttgaatg tatttccggc ctgtccggca cgggggcgac ggtcatcgac
     7261 gacatcgaag ccatgccggc cagcctggtc ctctggcggg ccctgaccca ctggttcggc
     7321 ggcctgggta tcatcgtcat cttcatcgct gtctttcctc agttcggcaa gggcatcgtc
     7381 cacatgttca atgccgaaag cacaggcccc cggtcggacc gggccctgcc gcgcatcaag
     7441 gagatggcca aggccctgtt cacggtgtac gtcgtcttta ccgttgcctc gacggtcgtc
     7501 ttcatgctct gcggcatgtc cttcctcatc gcagccgaca acgcctttgc cactatcgct
     7561 acgggcggtt tctcgccgta caataacagc gtcgcccatt tcgacagccc tgtcgtcgaa
     7621 tgctgcctgt cctttttcat gctcatcagc agcgccaact tcggcatgta cgtcgcggcc
     7681 tggaagaagg gctgcaaagt catcctcggc gatacggagt tccgcgtcta cttggccatc
     7741 gtcgccatct cgacggcggc catggccctc aacctggtcc tggcccagca gtgggacggc
     7801 atcgaagccc tgcgccaggc ctttttccag tccgtgtcca tttcgtcgtc gacgggcttc
     7861 gtctccaacg actttgaaca gtggccggtc ttttccaaat tcatcctcct ggtcctgatg
     7921 ttcatcggcg gctgtgccgg ttcgacgaca ggcggactga aagtgacgcg cgtcatgctg
     7981 ctggtcaaga ccattgccgc catcatccgc cagaagatac atcccaacat ggtcctgcac
     8041 atccgttcta atggcgaaga attctcgatg gacctcattt acggcgtagc caaattcttc
     8101 ttcatctaca ccatgctcgg cgtcctctgg acgttcatca tggtctttga cggcgtggcc
     8161 atcttcgatg ccatcggcct cagtgtctcg accatgggca gctgcgggcc aggattcggc
     8221 cagttcggtg cgacggctac ttgttcggcc ctgccgccgc tcagcaaagt cgccctctgc
     8281 ctgtccatgc tcatgggccg cctggaagcc ctgccggtcc tggctatcct catgccgtcc
     8341 ttctggcgcc accgcaacag ctggtaattg ggggaaatcc cttgcatgac gccatccctt
     8401 tgccgtataa taaagacaat cttaacgtta ctctatcttt tatcaaaggg ggattaccta
     8461 tgagtttttg ggaacaaatg gctaagacca tcgatgccag tgaagaaaaa cagaagaagg
     8521 aagatctcta ccagaagacc ttccacgaat tagccgaaaa attgagcgaa atccgcaaca
     8581 aagaaacggc ggacaagacg gaagattaaa ggattcttaa gtttttcgcc gttttccgat
     8641 ttttcattaa aagacgatta aattttgtca ggacgtctcc tttccatgct atgctagcta
     8701 tggaaaggag atttttttat ggacgtaaac aagttactgc aagacgccct ggctgccggg
     8761 gccagcgaca tccacatcga gcccctggac gacgccgtcc gcatccgctg gcgccgggac
     8821 ggtgtcctct gtcaggccgg gacgctccac aagggcggcc tggagaaact ggtaagccgc
     8881 atcaaagtcc tggccggcct ggacatcgcc aaccgccgcc tgccccagga cgtctggaag
     8941 gacggggaac gacgcctgga cctgcgggtc agtacgctgc cgacagtgcg cggcgaaaaa
     9001 gtcgtcatcc gcatcctcga cggccagcgc atccccctgg ctctctcggc cctgggcatg
     9061 gacgaccagg tccggcgctg tctggaaaag ctcatccgct gccgccaggg gctcatcctc
     9121 atctgcgggc ccacgggcag cggcaagacg acgactctct atgcggccat ccacgagatc
     9181 caggacgact cggtcagcat tgcgaccctg gaagacccgg tggaaatcct catggagggc
     9241 atttcccaga gccaggtcca ggccaaaggg ggcatgatgt tccaggacgg cctgcgggcc
     9301 ctgctgcgcc aggacccgga tatcctcgtc gtcggcgaaa tccgcgatgg cgagacggcg
     9361 cgcatcgccg tgcgggccgc cctgacaggg cacgtcatct tcagtaccct ccatgcgccg
     9421 agcgccgtcg aggccgtgat ccgcctgacc gatatgggcg tcgcgcccta cctggcagcc
     9481 gatgccctgg ccggcgtcgt gtcccagcgc ctggtccgct gccgcaggag cgatgggtct
     9541 tatcagggcc gtttctgcct gtgcgaagtc gtgccggctg gccgccgtct ccgcgacgcc
     9601 atccgccgct gtgccggcgt ccgggacctg acggatgcgg cccggtccga tggtgctgtc
     9661 ctcctgcccg acgtcatcgc ccggaccctg gcggccggac ggacggatga aagggagata
     9721 cgccgcgtct gtgaaggagg gagctcatgg tgaaggtaag ggaactgatg gaaacggcca
     9781 tgggtcagca ggccgcagac cttcatctgc agacgggccg gccgccgatg atgcgcctgg
     9841 cccagggcct ctgtccgctt ccagggccgc ccttgagcga aggggacctg cgccaggtcc
     9901 tgcaggccgt cggctggagc ggcggggacc agggcgacgg cgccttttcc tggcacgacg
     9961 ccctgcgctg ccgcctccac gtctgccggg agtacgccgg cctccatgtg acgatacgct
    10021 tcctctatcc tttagcgtcg ctgccgccag atggagacgg accgctgctg cagcgcctca
    10081 gctgcctgac cagtggcctg gtcctggtct gcgggcccac gggcagcggc aagacgacgg
    10141 ccctgtggcg catcctccag gccattaatg aacggcggcc ctgccatatc gtcaccctgg
    10201 aagacccgat tgaatacgtc gaagccggga aggccgcctt gattacgcag cgcgaactgg
    10261 ggacgcactt cgcgaccttt gccgaaggca tccgccaggc cctgcgccag gatccggacg
    10321 tcatccttat cggcgagatg cgcgaccggg ccgccatgga tgcggctttg acggccgccg
    10381 agacggggca cttcgtcttg tcgacgctgc acacgccgtc ggcagcacag gctgtgagcc
    10441 gcctggccgg tgcctatagt ggcgagaccc agcaggaagt ccgctgccgc ctggccatgg
    10501 tactgcaggc catcctggcc cagcgccgcc tggaacggca gggacggatc gacatcgtcc
    10561 gggaagtcct cgtccgcacg ccggccgtgg tccagctcat ccgcagcggt aaggaatatc
    10621 agctgccgac ggtcatgcag accggtgc
//
LOCUS       DAWABZ010000053        10571 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_1721_length_10571_cov_2.890262, whole genome shotgun sequence.
ACCESSION   DAWABZ010000053 DAWABZ010000000
VERSION     DAWABZ010000053.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 10571)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10571)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10571
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1721_length_10571_cov_2.890262"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7227..7236
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8144..8153
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2729 a         3007 c         2725 g         2090 t
ORIGIN      
        1 acttataaga aaggcttcga cgtattcttc gataccaatg acgtattcga tgaacggtcc
       61 atcgtcatca gcatgggcaa gaatttcttc tttgaaggct tcaaactctt gtaatcctgt
      121 catattgtag aatcaccgag aatgtggtaa aataaggaat aatcattgat agcgaggtga
      181 atatcatggc aaaacacatc attacaatca acacagaatc gatccagaag acggcacagc
      241 acgctggctg cagcgaatgc cagacgtcct gccagtccgc ttgcaaaact tcttgcactg
      301 ttggcaatca gatctgcaca aaataagtta cgtgaaggaa aaggggcgtc gctcacgcgg
      361 cacccccttc tttttgtatg gaggaaacat tattcatggc tgaaattaaa ccaatgattc
      421 ataaatactg tcagaacggg acgtacatgc tcctcgacgt caacagcggc atcatcaacg
      481 tcatcgacaa gatgacctac gatgtcctcg acgtctatga cggtaccaat aaagacgctg
      541 tctatcaggc ctttgccggg acgtatgata aaaaggattt ggacgaaacc ctgggcgaac
      601 tggatgagct catcgaaaag gaaatgctct ttgcaccgat gacggaaaac ttcaaggtcg
      661 tcgccgaaga agaaccggtc atcaaatcgc tctgcctgaa cattgcccac gattgcaacc
      721 tgcgctgtca atactgcttt gcctcccagg gcgattacga tacgcacaag cgggaactca
      781 tgagcttcga cgtagccaaa cacgctgtcg acctgctcat caagagcacc gaaggcaaac
      841 gccagcactg cgaaatcgac ttcttcggcg gcgaaccgct gatgaacttc ggcgtcgtca
      901 agcagaccat cgagtacatc cgggaacagg aaaagatcca cgataaagta ttcaagctgt
      961 ccctgacgac gaacggcatg ctcctcgacc cggccaaggt caagtatttg acggataatc
     1021 acatcagcct catcctgagc ctcgacggtc gtccggaagt acacgaccgc atgcgtcccg
     1081 atgccggcgg tcacggctcg tacaagacct gtgccgacaa tcaggtctat gcggcgaaac
     1141 accgcaacgg tgaagaatat tacgtccgcg ggacgtatac aaaatacaat ctcgatttca
     1201 ccaaagacgt agaacacatg gctgacctcg gctttgaagg cctgtccatg gaacccgtcg
     1261 tcggcgacga cttgtcctat gccatcaccg atgatgactt gccccgcatt tatgaagaat
     1321 acgaccgcct ggccgatttc tacctgaaac gcatggatga aggccggccc ttcatttatt
     1381 accacttcat catggacctc tatcgcggtc cctgcatcgc caagcgcctg cgcggctgtg
     1441 gtgccggcca tgaatacatg tgcgtcgtcc ccaacggcga catctatccc tgtcatcagt
     1501 tcgtcggcca ggacgactat gtcatcggca acgtctatga cggcgtgacc aatacggaac
     1561 tgccgccgct cttccgcgac atgcacgtcc tcaacaagcc catctgctgc gactgctggg
     1621 ccaagttctt ctgcagcggc ggctgccatg ccaataacat caagtacggc ggcaacatcc
     1681 agacgccgta tgaactgagc tgcaaaatcc agaagaagcg catcgaatgt gccatgtaca
     1741 ttcaggccgt cctggctatg cgcggccaga aagcccgcct cttcggggac ccggaagaaa
     1801 attgtgaagg atgcggcgcc tgtgaataag ctgaccaagc tcatgaagct caaggtcttc
     1861 ttatggatcg tcgtctatgc ctatttactc tatgccatcg tcgtcgacgg cccgtcgcca
     1921 ggtcttctag gcctgctggc ggcagctgtc gccgtctgtg tcatgcaggt atggatgcat
     1981 agcaaggccc gggcccggtg gatgcataat aaatttgata aaaaataacc agaaacgcta
     2041 ctaaataggt tgactttgtg gcgtttatag agtacattaa gaacagtcca gtaagtgtat
     2101 ctaaatggta cactttatca atacaaaaat tcgcgctctg tcctgctgtg caggggagag
     2161 gtgaaacgta taacttatgt aggagtgata ctgatgagtg aattattaag agtagaaggc
     2221 ttgcacatcg ctgtcgaagg caaagaaatc ctcaaaggcc tcgacctgac gatcaacaca
     2281 ggcgaaacac atgtcatcat gggttccaac ggcgccggca aatcgacgct gttcaatgcc
     2341 atcatgggca acccgaaata cgtcgtcaca gccggcaaga tcttctttga aggcaaagat
     2401 attacggaag ctcccgtcaa tgaacgcgcc aaagcaggta tcttcatggc cttccagcag
     2461 cctatcgccg tccagggcat ttccgttgaa aacttcatcc gcaacgccaa gagcaccgta
     2521 tcgggtcagc agcagcgcat catgcctttc cgtaagaagc tccacgccca gatggaacag
     2581 ctgaagatgg ataagtctta tgctgaacgc tatgtcaatg acggcttctc cggcggcgaa
     2641 cggaaaaaga cggaaatcct ccagatgtgc atgctcgaac cgaaactgac catgctcgac
     2701 gaaatcgact cgggcctcga cgtcgatgcc gtccgcatcg tatcggacac ggtatccaag
     2761 taccacaatg aaaacaattc catcctggtc attacccacc acagtgaaat cttgcagcac
     2821 ttgaaaccgg atttcgtcca catcctcatc gacggcaaga tcgtcaagac cggcgatgca
     2881 tcgctcatcc aggctattga agccaacggc tacgacgcat acaaaggata gtaggaggta
     2941 cagctatggc tgataataaa gaaaagctgg tcgatcagga aaaagaaaaa ctggtcgaac
     3001 agaaagaaga aaaaatcgct gaagaacgcc agaagatcga taccttcgcc gacttcggca
     3061 acatgtataa ctttaagaaa gattttacct attcctataa gaccgatgcc ggcctgacgg
     3121 aagacatcgt ccgtgaaatt tcggaaaaga aacacgaacc aaagtggatg ctcgatttcc
     3181 gcctcaaatc gctggatatc ttcaaccaca tggaatatcc cgactggggc ccggacatca
     3241 gcgaactcga catggacaac atcgtcactt atgtccgccc gaacgctcag atgaaagctg
     3301 actggaacga cgtccccgat gacatcaaag acaccttcga ccgcctgggt atcccggaag
     3361 ccgaaaagac gtccctcgcc ggcgtcggtg cccagtacga ctcggaagtc gtctaccaca
     3421 gcatccagga cgaactggtc cagcagggcg tcgtctatac cgacttcgat acggccctgg
     3481 aaaaatatga agacatcgtc aaggctcatt tcatgaagct cgtcccgccg acggaccaca
     3541 agttcgccgc cctgcacggt gctgtctggt ccggtggctc cttcgtctac gtcccggccg
     3601 gtgtcgacgt ctccatcccg ctgcagagtt atttccgcct caatgcgccg ggcgcaggcc
     3661 agtttgaaca cacgatgatc atcgtcgaaa agggagcgaa agtccacttc atcgaaggct
     3721 gttcggctcc gaaatacaat atcgccaacc tccacgcagg ctgcgtcgaa ttgttcatcg
     3781 gcgacgatgc atccctgacc tattcgacca tcgaaaactg gtctaagaac atgtacaacc
     3841 tcaatacaaa gcgggccatc gtcggcaaga acggcaccat caactgggtc acgggctcct
     3901 tcggttccca tacgtcgtgc ctctacccca tgagtattct ccagggcgaa ggcgcacact
     3961 gcgaattcac aggcgtcacc tttgccggca agggccagta cctcgatacg ggttcgaaag
     4021 tcgtccacaa cgcaccgaac acgacgagca acatcaattc taaatccatc agcaagagcg
     4081 gcggcgtctg catctatcgc ggcagtgtcg tcatcaaccc gccggctgac ggcgccaagg
     4141 ccaacgtcag ctgcgaatcg ctcatgctcg acgaacagtc gcagtccgat acgatcccgg
     4201 ctatcgtcgt caagaacgac aacatcgacc tcggccacga agcttctatc ggccgtattt
     4261 ccgacgaagc tatcttctac ctcatgagcc gcggcctcag cgaagatgaa gcgcgggcta
     4321 tgctcgtccg gggcttcgtc gaaccggtat ccaaggcact gcctttggaa tatgccgtcg
     4381 aaatgaataa cctgatcaat ctcgaactca aaggttcgtt gaaatagggg aggaggaaca
     4441 tcatggcagt aaagacagaa aaaatccagt acaaccaact cccgcgcccg accttccgct
     4501 ggatgaaggt caatcacctg gaattggacc ccttgaaagc ccaggctgct ccggcttata
     4561 cgccggaaga acgccacgac ggcgacgcag cggtgatctt ctatgaaggc aatcagatgc
     4621 cgggcctcgg cgatttccag ggttccaatg cggaagaact ccaaaaagca ctgaaccagg
     4681 ccaattctgg ctgcgccgtc agcgtcggcg aaaaccagaa agggtcggtc catttgaaat
     4741 acagcctcag cgaagcgatg cctcagctca cggctcagct caaaatcgaa gccgctgccc
     4801 acagcgattt gacggtctac ctggaatttg acggcagtgc cgatgacggc tcagtcaact
     4861 tcctcactta cgtcaagacc ggcgaagggg ctcacgtcaa agtcagcaag gtccagctca
     4921 acggcgaaaa cgtccgccac gtcgaacacc gctacggcga tgccggcaaa gaaagtacga
     4981 tcgactacgt cagtgccgaa ctgggcggca aggcagctat cgtctattac aagacagacc
     5041 tcatgcacga tgaaagcaat ttcaacagcc aggccatgta cctcggccat gacgaccagg
     5101 tcgtcgactt ctcttactgg gttcccacac agggcgtcaa gaccaatacg gacatcttga
     5161 cgacaggcgc cctcctcgat acgtcgaaga agttcttccg cggtactatc gacttcctcc
     5221 gcggcggcaa gaaagccgtc ggttccgaat cggatacgtg catcctgctc agcccgaagg
     5281 tccattccat ttccgtaccg ctcctcctct gcaaagaaga cgacgtcgtc gggaaccatg
     5341 cttccagtgc cggccagatc gataaggaca agctcttcta cctcatgagc cgcggcttta
     5401 atgaaaccgg cgcccagcta atcatcgtcg aatcgaatat ccgcccggtc atcgacgccc
     5461 tgggtgataa agatttagaa aataaagcgc tccaggctgt acgcgaaaag atgcagttct
     5521 gccgcaagaa aggtgattgc catgctaaat gtacgcaaag atttccccat cttgactaaa
     5581 gcccataata gacatcagat cgtttacttt gataacgctg cgacgacgca gaaaccgtac
     5641 caggtcatcc acgccatcgt agacctcttg gaacaccaca acggcaatcc ccatcgcggt
     5701 gcccacgtcc tcagcatcga agccggtgaa ttgtacgacg aagcccgcga agcagtccgc
     5761 aaattcaaca acgccgcttc gacggaagaa gtcatcttcg tccgcaatac gacggaagcg
     5821 ctgaacctcg tagctcgcag ctatgcagaa ccgcgcctga agaaaggcga caagatcgtc
     5881 atccccattt cggaacacca ttccgacctg gtcacgtggc agcgcgtctg ccagaagacc
     5941 ggcgccgtac tcgattatat gtacctcgac gaagaaggcc acttcacaga agaagacctg
     6001 gctaaaatcg acgacaagac gaaaatcgtc gcctttgccg ctgtcagcaa cgtcttcggc
     6061 atgaagcgcc cggtcaagga aatcgtcgcc aaagcccatt ccgtcggagc catcgccatc
     6121 gtcgacggtg ctcagtcgac accgcacatg aagaccgacg tccaggatat ggactgcgac
     6181 ttcttcgcct tctcgggcca caagatgtgc ggttctgcca gcacgggcgt cctttacggc
     6241 aagaaagcca tcctggaagg catggaaccg ttcctcctcg gcggcgacat gatcgaatat
     6301 gtcaaggaac agtcgacgac ctttaacgaa ctgcccttca aattcgaagc cggttcccag
     6361 aacgtcgaag gtgccgttgc tctgcacgct gccatcgact atttgcaaga cctgggcatg
     6421 gacaacatcg aagcccatga agaagaactg acccgccact gcctggaagg gatgaagaaa
     6481 atccctcata tccacatcat cggcagtacc gacccggctg aaaaaaccgg cgtcattacc
     6541 ttcaccatcg acggcgtcca tccccatgac gcagcgacca ttctggacag cttcggcatc
     6601 gccatcagtt cgggccacca ctgcgcacag cctttaggcg ctcacttggg cgtagaagcc
     6661 tcgaaccgcg ccagcttcta tatctacaat accctggaag aagtcgacta cttcctggaa
     6721 aaactgcccc tcgtccgcaa acagatgggc tttaaagatt aaggagatag aaagtcatgg
     6781 aaaatatgaa cgaattgtat tccgacatca tcttggaaca caatcagtgt caggaaaata
     6841 aacatcccct ggaagatgcc aacctcagcg aacacggcca caacccgagc tgcggtgacg
     6901 acatcaccct ccaggccgac atccaggacg gcgtcatcaa agacgctgcc tatacgggcc
     6961 acggctgtgc catcagccag gcttcagcgg acatcatgat cgacctcatc aaaggcaaga
     7021 ccgtcaaaga agccctgcgc ctggtcgacc tcttcctgga tatgatcaaa cgggaagtca
     7081 ccgacgatga ccagttggaa gaactggaag acgccatcgc cctgaagaat atctccaaca
     7141 tgccggcccg cgtcaaatgc gccgtcctgg catggcatac cctgaaagat gccctggaac
     7201 gggaagaaaa gtagccgcta gcagttnnnn nnnnnngcta gtggctcgta ggggacgccc
     7261 agagggcgtc ccgcttgtgg gaatcccata aatcaaaaga ggctgttgca ttaagcacat
     7321 gtttttgctt aatgcaacag cctctttttc gtgtacagag attcaggcgg gccgccagcc
     7381 tctgctgtcc gttccagcaa ccgtcctgcg ccttccgtgg gtcggcccct gacgggctgg
     7441 aaaggctttt aggacggccc atgcggtgct tgcggcctgc aggctgcgag ccacgaacca
     7501 ctagccactg actttttcat ccacgctgcc tttccactgg atgaggatga aggcgatgag
     7561 ggcgatggcg aaggcgatga tgctggtcat ctgggccgat ttcaggccca gcgtcaggtt
     7621 gacgtagtcg ccacggagga attccaggaa gaagcgttcg acggcgtaga gcatgacgta
     7681 caggcaaaat acctggccct tcttgtgggg gaagcagctg tagaggagca gcgcgacgaa
     7741 gaccaggacg tcgatctgac cttcccagac ttcggccggc cacaagggct gattgccata
     7801 cgtgtgatag gccagggtcg tgtcgggata gacgatgccg aagtcaccgc ctgtgggatg
     7861 gccgaaggcg tcgccgttca tgaggttggc catgcgcccg atggattggc ccaggatgat
     7921 ggacggtgcc aggaggtcgg caaaggccca ggtgtcgatg tgatggtact tcgtgtacag
     7981 gatgccggct acggcgccga agagcaggcc gccctggata gccatgccgc cctgccagac
     8041 gtaggggatt tccgtcagat ggttgtgata ataggcccag tcgaagaaga agacgtccca
     8101 tagacggccg ccgacgatgc ccgacaggcc gcagtacatg gtgnnnnnnn nnnagtcaaa
     8161 gatgtggacg tgccagcccc ggccgtcgcg tttcatgata tagtaagccg taatgccggc
     8221 agaaacgata cccagcatga ataacagccc ataggcccgg atcgggaagg aaccgataaa
     8281 aaatagatat tgatgcaata taaaacaccc ctttgttgtg gtataatgaa acaatgtgaa
     8341 cggaacatga aaaggttcac ggttaccata gaattataca ggattgagga cttttttcca
     8401 atccccgtga ggagcaaatg acgatgaaat acaaccatgt tatgcggacg tcctgtctgg
     8461 ccgccttgat ctgtctgtcc ctgagtgctg tttcctgggc caatgggccg gctgcggaca
     8521 gcgccaaggc gtcggacccg gccttcagtg tatctaccgt agccaatcct atccctgatg
     8581 aagccatcaa agacctggaa atcgtcgaag accaggggcg ctggctcgtc tctgacaagg
     8641 cgctggaaca atacggcatc cgtgaagccg accacgccaa ggggacctac tggttccgcc
     8701 tgccgaaggc ccagaacggc tcgacggcct ggcagaacga aaacgtccag ctgactctgc
     8761 caggacgagc agtcagcggc ggccggacca tcaatatccg ccatgtccgc cgtcccctgg
     8821 gcatcagcta cgtcctgggc aacgggacga cagaaaagaa cgaactcctg ccgccatccc
     8881 agcccgtcca tacggtaccg gaactccgcc aggtacagga cgcagtaccg gcagcggcta
     8941 aggaagggaa gatggggacc gtcctctttt gggaccctgt catgaaggaa gacgccagcc
     9001 tgccgtcttt gcagacccgt cagcccgtca tgtcgccctg tgctttccgc ctgacggaaa
     9061 gcggcgtcga actgcgcaat cccgacttcg atatgctggc ttcgacgtat accagcaagg
     9121 gatacgttat gtggcctttg atcgacaaca atttcaaccc caaactgacc catcaggtcc
     9181 tgagcaacag ccgtctccag gacacgatgg tccgccagct cgtcggctat gccttattat
     9241 atgatttcaa aggctataac atcgactttg aaaacgtcaa ttacagcgat aaagatgctc
     9301 tgacggtttt tgtggccaaa ctgtcccagg cctgccatgc ctatggtatc cagctttcca
     9361 tggatgtcac gcccgtctcg gacagcccca actggtccct ggtctatgac cgggcggccc
     9421 tggctccgca tctcgactac ctcatggtca tggcctatga ccagttcggc cgggccagca
     9481 cgacagccgg ccctgtcgct tcctatccct gggtcgaacg ggctgtacag aatacgatcg
     9541 atctcgtacc gccgtcgaaa gtcctgctgg gcatgccgtt gtacatgcgc ttgtggtatg
     9601 aatccagcga tggccgcgat ttgccgaaag acatccacga ctggccggct gtcgtcggcg
     9661 caccgccggc aaagggcgtg aaatacagcg aggccagaga agaagcagct gaagaagcgg
     9721 ctgggccgat gctggtccag gtcgtaacgg cggaacagga aaatgtccgt cgggccaaac
     9781 agccggtcat catgcctgtc gaactcgatg gggaaacgta taagcgccgt gtcctgccgc
     9841 gtcgtatcag gacggcaccc aaagctgcag ccacggctcc ggcgcgcaag agcggcggca
     9901 agggcaagct cttcgtccgc acgttgacca tggccgacag cgaagacatc ctcaaggcct
     9961 ataaggacta tgtcgtctgg aacgatgatc tgcagctcta ttatctggaa ctgccgctga
    10021 cgacggggac cgtcaagatc tggtttgaag atgaaaagtc actgaaagcc aaagccggcc
    10081 tggtccagac ctatggtttg ggcggcgctg ccttctggcg caaaggcttt gaaccggcac
    10141 atttttggca gcgttttgca aaacatgaat tgacttgacg acgccctgcc actataataa
    10201 tactcaagac gctgtagtac agtctgtctg tattttgact ggggcaggat aggaggctct
    10261 cacaattgga aaagtatatt ccacaggaaa tagaaaagaa atggcagcac gtatgggaag
    10321 aaagtaaatg cgactgctgc accgaagaca acggcaagcc gccgtactac gtattggaaa
    10381 tgttcccgta tccgtcgggt aacctgcaca tgggtcatgt ccgcaattac acgattggtg
    10441 acgtcattgc ccgttatcgc cgcatgagcg gctataacgt cttgcatcct atgggctatg
    10501 atgctttcgg tatgcctgca gaaaacgcag ctatcaaaca caatatcccg cctgcagaat
    10561 ggacatacag c
//
LOCUS       DAWABZ010000054        10276 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_1782_length_10276_cov_3.460033, whole genome shotgun sequence.
ACCESSION   DAWABZ010000054 DAWABZ010000000
VERSION     DAWABZ010000054.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 10276)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10276)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10276
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1782_length_10276_cov_3.460033"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2402 a         2837 c         2776 g         2261 t
ORIGIN      
        1 atgctaatca ggagcgcctg atggaaaaac tgaggggaga agaacaattc ctggtcatcg
       61 attcgcatac catgggagaa ccgacgcgta tcgtcgtcaa tggatttcct cacgcgccag
      121 gcaggacgat gatggaaaag aagcagtaca tcgccaagca ctacgatgcc tatcgcaagg
      181 ccatcttgct ggagccgcgg ggccatacga atatgttcgg cgccatcctc atgacgccgg
      241 tccacgaaga agctgatgtc ggcgttgtct tcatggacag cggcggctac ctgaatatgt
      301 gcggccacgg ttccatcggc gtggcgacgg tgctggtcaa tgaacagatg atacccgtcc
      361 atgaacccta tacggacatc tgcctcgatg cgccgtcagg cctcatcaag ggccacgtca
      421 aagtcgaagc aggccgggcc gtagaagtga ccattaccaa cgtgccctgc ttcttgtacc
      481 ggacaggggt cacggtccct gtcgacggct taggccacgt tgccctggac atcgcctttg
      541 gcggcagttt cttcgccctc gtcgacaccg atgctttggg gatgcgctta gagcctgaaa
      601 atatccggac tatcatcgac acgggcatga acatcctccg ggccgtcaac aatcaggaag
      661 tcgtagccca tccggaactg gacatccaca gcgtcgattt agtcgaattt tatggtcagg
      721 ccgatcggcc cgaagcgacc atgaagaacg ccgtcgtctt cggcaactac cagatcgacc
      781 gcagcccctg cgggaccggc acgagtgcca aagtggcgtc gctgtacttg cgggggaaat
      841 tgaagaaaaa tgaactattc gtttatgaaa gtatcatggg aaccttgttc aaagcccgca
      901 tcgtagatga tgccgaagtc ggcggcatgg ccgctgctat cccgcagatt acaggcagtg
      961 cctatattac cggcatcaac tgcctggtcc tcgatgagac ggacccgttc cgccacggct
     1021 tccagattca agcctgaata tacagttccg gtcatgagac ctgaatgata gattccagtc
     1081 atacatgtga agaggggtgc ctgattatga tgatgaacag taaggtaaaa aaagcaatct
     1141 tagccggcct gagtgccgtc gtatccatat ctgtcttagc tggctgcggc ggaggtagcg
     1201 gcagcagcga taaggccagc ctgaaaggga aagagctgaa agtagcgacc gggatctatg
     1261 cgccctttgc tttcaaagat gaaaacagta agctcgtcgg cttcgacctg gacctgctcg
     1321 atgccatgtc caagaaactg ggctttacct ataaagtcac gccgacggaa tatgacaaca
     1381 tgttcatggc cgtcgccaac ggtgagtacg atatcggcat gggccaggtc tgcattaccg
     1441 aagaccgcaa gaagaagatg gacttcaccg aaccctatga aaacgcaggt ttgcagttcg
     1501 tcgtccgcaa agatgaaaac cttacgtctg tagatcagtt gaaaggcaag aaaatcgccg
     1561 tcgaaaaggg gacagctgcc cacaaatacg taacggaaca ctgtaaggat tcggaaatcg
     1621 tcgtcttccc ggctatttca gcagccttca tggaagtcga acagggcaag gccgacgccc
     1681 tcatgcagga taaaccgaat gccgtctatt atttgaagaa gcatcctgaa agcggtctgg
     1741 ccctcttggg cgatgaaacg gccaagattc ccgatggctt cgccctgcct aaggacagcc
     1801 cgtataaagc tgaattcgat aaggccctga aagaattgca ggaagacggg acaatcaaga
     1861 agttagaaga taaatggctg aatgtctaac ggcagcagcc agtcagcagt ggcccggcaa
     1921 gagtcgtact tttgccggac ttcttgctat aaggggtctt ttattaaggg gggaatctta
     1981 tgtttaactg ggaacacttg gacatcatcg tcggtgttgt tacgaaaggc ctgttctgga
     2041 ctgtcgtcat ttccgtgacg agtatcatca tcggctattt catcgggctg gctacggggt
     2101 tttcccgggc ccgcaatata cgcatcctca agagcatcag cgctttctac gtctggatca
     2161 tccgcagtac gccgatcctg gtccaggcct tgtacatcta tttcgtcatg ccaaaactct
     2221 ttggcatcca tttatctcat gaagcggcag gcatcctctg cgtttccctc aatgccgggg
     2281 cgtatatatc ggaaatcgtc cgcggtgcca tcctggaagt gcctaaaggt cagtgggagg
     2341 cgggcatgtc cctggggatg acgaagctgc agatcgtcat gaagctcatc ctgcccatgg
     2401 ccttccggac catgctgccg gccctgggca accagttcat cattaccgta aaggatacgt
     2461 ccattttgac ggtcatcggc gtcgccgaaa tgacctatca ggctggccag tacgcttcgt
     2521 cgaactttga attcgtcgaa agctataccg tcctggccgt attctactta gtcctgattt
     2581 cgctgctgac cgtagccgtc aatatttgtg aacagaagat gggagctgat ataaaatgat
     2641 gcgaattgaa catttaaaga aacagtatgg tgataacctg gtcctgaatg acatcagcct
     2701 gtccctggac gatgaagtca cctgcatcct cgggccgtcc ggcgccggca agagcacctt
     2761 cctgcgctgc ctgaacctgc tggaatcgcc gacaggcggc gacatcaagt acaacggtca
     2821 gagcatcctc cagccgggct acgatatccg caagatgcgc gaagaagtga ccatggtctt
     2881 ccagcacttt aacttatttc ccctgcgcaa cgtcgtcggc aacgtcatgc tggccctgca
     2941 aagcgtcaag cacatgaaga aagccgatgc ccacgatatt gccatggaaa tactaaaaaa
     3001 ggtcgacatg gccgataagg ccgaaaagat gccggatatg ctctccggcg gccagcagca
     3061 gcgcgttgcc attgcccgcg ccctggccat gaatcccaag atgatcctct tcgacgaacc
     3121 gacgtcggcc ctggacccgg aactcgtcgg cggcgtcctt gacgtcatgc gcgacttggc
     3181 ccataacggc atgtccatgg tcatcgtgac ccatgaaatg gcctttgccc gcgacgtatc
     3241 agaccgcatc gtcttcatgg ccgacagcat cgtccaggaa gatacgacgc cggctgaatt
     3301 cttaagccac cccaagacgg aacgggcccg caatttcctg agccgcttct tatcgtaagg
     3361 gagggagtac gatggaaaaa ttcatcatta ccatcaaccg ccagttcggc agtatgggcc
     3421 ggcccattgc caaagaaatg gccgaagccc tgaacgtcga attttatgac cgcgacctca
     3481 tcgaagctgc agcgaagaag atgaatatgt ccttgtctat tgccagcgac ctggaggaat
     3541 ctgccaggcc gaaccgcttc tggcgcatgt gcttcccact ggggcacgac gaatcgcgga
     3601 aacaggacga actcttccag gtggaaaaga aaatcatcct ggacctggcc gatgccaagt
     3661 cgtgcatcat cgtcggccgc tgttccgatt ccatcctgaa atgttccaag cacttgctgc
     3721 gcatctttat ctatgcgccc tataaagaac gcctcaagaa ctgcgtcgaa cgcctcaaga
     3781 tggacgaacg gacggccaag gaaatgatac gcaacgtcga ccgggcccgc gatgcctacc
     3841 aggtgcgcta cgccggctat ctgccaggcg acttccacca tacggatctg ctcatcaaca
     3901 gcagcttcct cggcgtcgaa gcgacggccc agtatctgac cgaactggta ctcaaaaaat
     3961 acggttccca tcatatcgaa gaagtatgaa aaggctcccg ccagggacat gctgtcttgt
     4021 tattgacaaa tgagattacc tattgcatga tagtgataat actaagaggt atcagacaaa
     4081 ggagatgtct tatgggcggg agcaaaatct gttatatcta tttcgtgctg agtcaggcca
     4141 tctggggcgt actgccggcc ttctggctgc tgttgcggca gctgccgccg ctgtatacgc
     4201 tggcttcgcg gattgtctgg gcatcggtct tctgcttttt actgatattg cagaaaaagc
     4261 tcctgcccga cctgaaaggg gtccgacagg agcgctggca atggccttat attgccgggg
     4321 cctgcatctt tatcaccctg aattgggggg cttatattta cgccacgaca cagggcttca
     4381 tcctccaggc cagcttggcc tatttcatca atcccatcat cctcgtcttc tttggtggtc
     4441 tgatcttcca tgaacggctg cggccggtcc agaaactgtc cgtcgtcgtc gccgctaccg
     4501 gcctggtcct ggccttctgc ctgtacggcg tcgtcccctg gctgtcgttc ttcatctgcc
     4561 tgagttgggc gatatacagc atgttgaaaa agaaagtccg cctggacagc caggtttcgg
     4621 tcttcatcga atccttttcc atggtgcccc tgtccctgct gttcatcgcc ttcagcgaat
     4681 atcatggcat gggggctgtc gggactttcc agggctggca gtggctgctc ctgccggcga
     4741 cgggcatcgt cacggccatt ccgatgatgc tcttttcggc cggcgtcaaa ggcgtcccta
     4801 ttacgacagg cggtatgctc atgtacctct cgccgagcat cactctggtc atcggtctct
     4861 tgagcggcga aaccatcacg ccgcccctgc gcctgacctt ctgcttcgct tgggttgccg
     4921 tcgccctgta tatgtacggc ctttaccaga ctatcaaagg gctccgggaa agcggatacc
     4981 attaggtacg atccttgctc ctgaatgacg ctgtaggggc tcgcacgtgg cgagcccgct
     5041 ccaggatatg tacgatttcc cggttagttt gctaagagaa cgattcaaat acccggcggg
     5101 tcgcccacgt ggcgacccct acagaaaaaa gggtgtcgta cgaggtttgt gccaccatcc
     5161 tctaacacct aacccctaac aactaacaac tagccactaa caacttgaaa ggagctgtcg
     5221 cgatgcgaca gctccttttg tatcacttac tttcaaaaaa tgctttgtat gccaggtagc
     5281 cgctgtaggc atcggctttg gcgacgattt ctaagggagc gtgcatgctg tgcatggcta
     5341 cgccgcagtc gacgacgtcg cagccccagt cggccatgat gtaggcgatg gtaccgccgc
     5401 cgccctggtc gaccttgccc agttccccga cctgccaggg aacttctttt tcgttgaaga
     5461 tgcggcggat cttggccatg aattcggcgt tggcatcgtt gctgccggcc ttgccgcggg
     5521 caccggtgta tttgcacagg cagataccgt agccgagctt ggccgaattc tgttcttcat
     5581 aggcgtcggc aaagaccggg tcaaaggcgg cgcagacgtc ggccgacagg acttcgctgt
     5641 tgcgcagggt ccggcgcagc cagatttctt ggtcatggcc ctggagggac aggagttcgg
     5701 cgataaagtc cgggaagtac gacgattgga cgcccgtgtt gccgacggaa ccgatttctt
     5761 ctttatcggc aaagagggcg acctgtgtct tttcccccgg tgcggcatcg aggatggcgg
     5821 ccaggttggc ataagagcag acgcggtcgt catggccgtg ggacatgatc atcgaccggt
     5881 ccaggccgac gtcgcggctc ttgacggccg ggatgatttc cagttctgca ctggtgaaat
     5941 cttcttcttc aataccgtat ttttccttta acaaggtcag gatagccggt ttcgtctttt
     6001 ctccttcgcc gttcgtgccg gccaagggca tgagcatttc gccgctgatg gcttcagaga
     6061 gctttttggc ggcctggttc ttgcccaggt ggggcagaag gtcattgatg aacaggaccg
     6121 gatcgcccgg gtcttcgccg atagagacgt cgacgcggct gccatcggct ttatagacga
     6181 cgccgaccag ggacaggggc aggcagaccc attgatattt cagcaagccg ccatagtaat
     6241 gggtcttgaa atagacgatt ttattctttt cgacgacagg catggccttg acatccaggc
     6301 gcgggcagtc gatatgggag ccgacgattt tcagcccttt ttcgatgggg gccgagccga
     6361 tgacggctaa gatgaccgat ttccctttct ggtcccaata gaccttgtcg ccggccttca
     6421 ggctggtgaa gtcagccagg ggatggaacc cggcgttttc agcctggcgg atgatttctt
     6481 tattggccag ccgttctgtc cgggctgtgt ccaggaactg acggtaatgg acgctgtacg
     6541 cttccatctg gccgcgctgt tccggtgaga agcgggacca gacgctgttt tctgtatcaa
     6601 atgctttcat gatataagcc tcctctgtaa atgatgatgt gtgacactgc atccttatta
     6661 taacgcactt atgggagctg tactagttct tgaaaaagac aggggaaacc gttgtatttc
     6721 tttatattgt gtggtattat taatcatact ttataaataa taattcatac atgggagggt
     6781 ttgccatgat cgataaaatg actatcaaag acttagtgaa gcggactgtg cctgatattt
     6841 gtgccatgcg ccgtcatatc catgaaaatc cggaattatc cggcgaagaa gtggaaacgg
     6901 ctgcctttat ggcagccaag ctgcggtctt tcggcatcga tgtacaggag cacgtcgccg
     6961 gaacgacggc ggtcctgggg acgctcaaag gcggcaagcc ggggccggtc atcgccttgc
     7021 gggccgatat ggatgctctg cccatgacgg aagaaacagg cttgcccttt gcgtccaatt
     7081 tcgtcggcaa gatgcacgcc tgcggtcatg acggccacat gagcatcctc ctcggtgccg
     7141 ctgaggtcct gagtcaggtg aaagacgata tccccggcac ggtcgtcttc atctgccagc
     7201 cgtctgaaga aaaatcgcct gtcggcggag ccaagggcct ggtcgcctcc ggcgtcctcg
     7261 acggtgtcga tgccgtttac ggccttcacg tctggccgac actgcccagc ggcaaaatcg
     7321 gtgtcctgcc cgggccgatg atggccgctt cggaccatat ccgcatcgtc ctcaacggca
     7381 aggccagcca tgccgccatg ccgcataaag gcgtcgacac catcgtcgca gccgcccagt
     7441 tcctgacggc ggcccagacc atcatcagcc gccgcgtcaa tcccctctat ccggctgtcc
     7501 tgacttttgg caaaatcact ggcggcacgc gctacaatgt cgtcgccgat acggtcgaaa
     7561 tcgaagggac ctgccggacg taccatgcgg aggcccagga tgccgtcgaa gcccatctcc
     7621 aggatacgct caaaggcatc gatgccatgt acgggacggt cagccatctc gactatgaac
     7681 ggggctatgc ggctgtccgc aatacgccgc agcaggcggc tttcgtcgct ggcgtagcca
     7741 aagactgctt tggcccggac gttctggcag ctgtcgacga accggccatg acggccgaag
     7801 acttttccgg ttatctccag aaattcgatg gcggtttctt ctggctcggt gcgaccaagc
     7861 ccggcgacac ggtttatccc ctgcacaata cccattttgc cgtcgatgaa gcctgcctgc
     7921 cagtcggcgt cgaactgatg gtttccctgg tcctggccaa aatgaaagaa aaagcataat
     7981 tatttttgaa aaaatgcaac ttaaaaaagg aatctgcccc cttcatctcg aatatataat
     8041 attaactgag attccatagg aggcgattgt tttatgaaag aatacacaag tgataaaatt
     8101 cgcaacgttg ctgtactttc gcatgatggc gccggcaaaa cggctgttgt tgaatcatta
     8161 ctgcttgcct gcggtgctgt cgatgctgtt ggcgtcggta aagacaacaa gcatattatg
     8221 gactatgaac cggaagaaat taagagaaac gttacgatcc agctcggcat tgcgccctgc
     8281 gaatgggaag gctacaagct gaacttcctc gatacgccgg ggtattccga attctgcggc
     8341 gaagtccgcg ctgccctgcg tgccagtgac ggcatcctgc tcgtcgtttc ggccgaatcg
     8401 ggcgtcgaaa tggatacgga acgggcctgg gactacggcg tcgaattgaa attgccgcgc
     8461 atggtctatg tcaacaagat ggatgcggaa cacgccgatt tcttcggctg cctggaaaaa
     8521 ttgcgcgccg tcttcggcaa atccatcatg cccctccaga tccctatcgg tgaaggcaag
     8581 gatttccggg gcatcatcga cgtctgcaag atggtagcct gggaatggaa taacggcgaa
     8641 tgcacggaaa tcaaggtgcc gccagaatac atggccaaag cccgtgaagt ccgcgaaatg
     8701 tgcatggaag ccgctgccga aggcgacgac gaattgctgg aaaaatacct caacggcgat
     8761 gaactgacca tcgaagaact gcgcaaaggc ctgcgccagg gcatgctgac gggccgcgtc
     8821 tgcccgctca tgtgcggcag tgccatcaac catatcggta cggcagaact cctgcgccgt
     8881 atcatcacct acatgccgga tgcttcgcag aaagtcatga tgggcacgga taagaagaca
     8941 ggcgaaccgg tcctggtcta tcccaacaag cctttcacgg ccttcgtctt caagaccctc
     9001 atcgatccct ttgccggcaa actgagttat gtccgcattt tctcgggcga actcaaggaa
     9061 ggggacaagt tatacaatat gtcccaggat aaagaagaaa agatgggcaa agtcctgacc
     9121 ctggtcggca aggaacagat tcccgtcccg gcggctattg ccggcgacat cgtcgtcctg
     9181 ccgaaattgc cgaatgcccg gactggcgat acctttgccg ctcctgattt ccccgtcgtc
     9241 tatgacccca tccgcttccc gaacccgctg catacggtag ccctggtccc ggaaaagaag
     9301 ggcgaagaag aaaaattgat gaacgccctc ctgcgggtca gcgaagaaga cccgacgtgc
     9361 caggtcgaaa agagcaccga aggcaagcag ctcatcgtcc gctgcatggg cgatgtccac
     9421 ctcgaccaca tcctgaccaa aatcgaacgg aaatacggtg tcaaagccaa acaggaagac
     9481 gtttacatcc cgtatcgtga aaccatcaag agcaaggcga cagccgaagg caaacacaag
     9541 aaacagagcg gcggccacgg tcagttcggc cacgtcttcc tggacatcga gccgctgacg
     9601 acaggcgaac cctttgaatt cgtcgataag atctttggcg gcgccgtacc gaaacagtac
     9661 atcccggccg ttgaaaaagg cgtccgtgaa accttggaaa aaggcctcat cgccggcttc
     9721 cccatgatca acgtcaaagt taccctgacc gatggttcct atcatcctgt cgattcgtcg
     9781 gaaatggcct tcaaagtcgc tgccgctcag gccctcaaga aagccgtacc tgaagccaag
     9841 ccgatcctcc tggaaccgat ttacaacgtc gacgtcgtca tccctgaaga ttacatgggc
     9901 gatgtcatgg gcgacttcag cagccgccgc ggccgcatcc tcggcatgga acaccatcgc
     9961 aaccgcaaag gcatcatcgt cgtcaaagcc caggtcccgc tggcagaaat gaaagactac
    10021 gtcaccgtcc tgcgctccat gacccagggc aaaggcacgt tcaacatgga attcttcgac
    10081 tacgaagaag taccgaaaaa aatcatggac gaaatcatcg aaaaaagaca aagtgaataa
    10141 gactagtggc tagtcgttcg tagtttttag tttgtagaaa tgggctttaa gtttttaaac
    10201 atccgctaac cactaacaac ttaaaagggc ctgtcgcacg taatcgtgca caggctcttt
    10261 ttatgtatgc aaaaac
//
LOCUS       DAWABZ010000055        10086 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_1824_length_10086_cov_3.291297, whole genome shotgun sequence.
ACCESSION   DAWABZ010000055 DAWABZ010000000
VERSION     DAWABZ010000055.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10086)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1824_length_10086_cov_3.291297"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2582 a         2743 c         2662 g         2099 t
ORIGIN      
        1 gattttcccg gtccacgacg aaaatcatca tacccatttg atgatcggtg aaatcatgca
       61 tttcgaagac acaggcaaca aggacgaaga cattgcccgc atgacccgca agatggccgt
      121 cgtcacggag aatttcatca aggaacaccc gacagaatgg ctgtggttcc agcatcgctg
      181 gagtacagaa ccggaagaaa tcatagcact gcagcagaaa ccggaggcgc ataccgatga
      241 tagccgaaaa gctgaaaaag aataaaatct ccatcattgc cgtcatcgcc gtcatcggct
      301 ttgccgtcat gctctatagt ttcatgcatc cccagaagac ggaagacccc atggtcagcc
      361 cggataagct cgtcgaattc gaagggacgg agctggaaga aaagaaagac ggccagctcg
      421 tctggcgctt gacagccgat aagatccaga tcgacccgga cacgcagatc atgtatttca
      481 ccaaccccaa ggccctggtc gtcgccgaag atggaacgca gatgaccatt acgtcggaca
      541 agggcatcgt cgaccgccag aagcggacca tcgaaatcaa gccgcctgtc aaagcagaaa
      601 ccgataaggg cgatacgttg cagaccgacg gcagtgtgta ttataatatg gatacgcgta
      661 tgatcaaagg cggcaaggtc gtcatggacc gccatgacaa gacgtcgctc aaagccgatg
      721 cttttgaaac ggacagcagc ctgaccaagg tgacgctgac cggccatgcg caggtgacaa
      781 agggagaata actatgatgg atttacttgg aaagaaactg gcagctgccg ccctcatggg
      841 ggccatgatc ctgccggctg ccggcgccct ggcggcggat aatgacggca acccggccgt
      901 atccgtcacg gcagacacgc tggaatataa cggtaagacc caggtcgcta cggcgacggg
      961 caacgtcgtc atcgtccgcg accaggctac gatgaccggc aacaaggccg tctataactt
     1021 gaagaccgcc gaagcagaca tggaaggcaa cgtatcggtc cagcagccgg acatgcagct
     1081 caacgccgat aaggtccatt cgaccaacaa gaattacgtc gtcgctacag gcagcgtccg
     1141 cggcgtctac ggcgacaaga aggtcaatgg cgacaaagtc gaatactatc tcgaccagga
     1201 ttacggcatc gtcacgggca acggctatct tgaagcccag ggttcgcaga tgtgggccga
     1261 tcacatcgac gcctggttca agcagataaa ggccatcggt acgggcaacg tccatatcga
     1321 atcgccggcg gaaaacctca tcgcctatgc caatcaggcc gtctacaccc agacgccggg
     1381 gcagaatgac ggcgtcatcc acttgacggg gaatgtcaat gccactcaga acggcaattc
     1441 tctcaacggc gacaatgtcg atatccgcct ggccgataat tccgtccaga cccgcagccg
     1501 atcgactatc gtcatcgtac cgggcagtaa tccgtaatcg taaggggtat ctatgtttat
     1561 tcagactgaa gatctcgtaa aagcgtataa agaacgaaat gccgtcaacg gcgtcagtat
     1621 ccgcgtcgac cagggccaga tcgtcggcct cctgggaccg aatggcgccg gcaagacgac
     1681 gactttctac atgatcgtcg gcatcgaacg gccgacgtca gggaagataa ccattgatgg
     1741 caaggacatc accaacctgc ccattcacaa gcgggcggcc tatggcatcg gctatctgcc
     1801 tcaggaagcg tcgattttcc gcaagctcac cgtcgaagac aacctcctgg ccatgctgga
     1861 gacgacgaag ctcagcaaga aggagcagca tgaaaaagtg gaagccctga tggaagaatt
     1921 ttccatcacc cagctccgcg accgcctggg tatccagctg tccggcggcg aacggcgccg
     1981 cgtggaaatc gcccgctgtc tggtcatgga cccggccttt atcctcctcg atgaaccttt
     2041 tgccggcatc gaccccctgg ccgtcaacga catccagggc atcatcagcc atttgaaacg
     2101 gcgcggcatg ggcgtcctca ttaccgacca caacgtccgg gaaacgctga gcatcgtcga
     2161 caaagcctat atcttgagtg aagggaagat cctcctcgaa ggcagtccgg aaatgattgc
     2221 caatgacccg gtagccaaga aattctactt aggcgaagaa ttcaggatgt aacagggagt
     2281 atggaatgcg aatattagat aaatatatac taaaagaatt tattggcccc tttttattcg
     2341 gcgtctgtgc ctttacagca gtcttcgtcg ggacgggtac gctctaccgt attgccaacc
     2401 tcatcaacca gtacggggct tcggcctggg ctgccttccg cgtcttcgtc ctggccatgc
     2461 cgtcgatcat cgtcgtcacc tttcccatgt ccgtcctctt agggtcgctc atggcctttg
     2521 gccgcctttc cagttccagt gaaatgatcg tcatgcgggc tggcggacag aacttcatcc
     2581 gcctggccat gccgattttc attgccgctt tcttcatttc catcggggcg acggccttca
     2641 acgaatacgt cgtgcccaag gccaacaacg cctataacac gatcatcaat gaagaaataa
     2701 agcacaatgt cgcgccgacg acgcaggacc atatcatcat caagaacacc aagggctccg
     2761 atatatccag cctcatgtat gcccgtcagt acgacgccaa cacgaagcag ctcaaggata
     2821 ttaccgtcca ggaatttgaa aatgatacgc tcatgcgcgt cgaacaggct gaccgggccg
     2881 actggaacgg ccagaaatgg gtcatgcacg aaggcatgat ctatgacgta tcggccaatg
     2941 aacagacgac gcggtccctg aagttccaga accaggccct gccgattacg cagaagccga
     3001 ataagatcaa tgcatcccag aagaaaccgg atgaactgac catccgcgaa ctgcgggaac
     3061 agattaaact cttggaccag aattccgtca acaccaataa gatgaaagtc gaaatgtaca
     3121 accgcttcgc cctgcctctg gccagcctgg tctgcgccat cgtcggcgcg cccctgggga
     3181 tgcagaaaca gcgcggcagt tcgtccatcg gctttggcat cagtgtcgtc gttatcttta
     3241 tttactattc catcatgacc ctgggcaacg ccctgggcaa tggcggcaag ataccggcct
     3301 acctcgccgc cttcctgccg gacatcatct gtggcgtcgc cggcgtcatc ttagtctaca
     3361 aaaaatcgaa atgagggaaa aggcgctgtc gcatgaaggc agcgcctttt gagtattaag
     3421 tttgcagttt gtagttttta gcgaatggtt ctaaatttaa agccatctac tgcaaactac
     3481 aaactctgtg ctacaaacta taaagcgaaa ggggcctcgc ataatggtag aaaccctcgt
     3541 gcgacagccc cttttaagag gctgggagct aacagccagc tgctaacgac tagggcagga
     3601 cggcccttgc agtacacggt agttatgggc tataatataa tgatataaag gaatgagcaa
     3661 aggtggtgtc ttatggaata tggatgggtc atgctgatcg tcttgtctgt tatgggcggg
     3721 atcattgcct atctcggcga taagatcggc tcccgtgtag ggaaacagaa aattaaatta
     3781 ttcggtttgc ggccgaagta tacgtccatc ctggttacca tcctgacggg gatcagcatt
     3841 gccgtcatca ctttaggcgt catgtctatc ttgtcggaaa atgtccgcat cgccttgttc
     3901 ggtatgcacc agctgcagat gcagaaggcc cagctcgaag cccagcggga tgaactgctg
     3961 cagcaggcca aggacctggg caaggaactg gacgataaga acgagctcat cgcctccaat
     4021 gaagaactcc tgaagaaaca gcaggaacag ctggacggca agaatgaaga aatccggacg
     4081 actcagctgg acctgcagca ggcccaggcc gcccgcgacg acaaggcccg ccaactgacg
     4141 gtcatccagg ttgccatgga cgaagccaag aagcagcgcg actcggccct ggccgatagt
     4201 gaaaaggccc gtcaagacgt agctatgctg gaagaaacga agcagaagat gatgggcacc
     4261 atcgacgtcc tcgacaagca catcaagctg ctcaatgaaa cgatgaccaa tatccgcgaa
     4321 gggacggtcg tcttccgcgt cggcgaagtc ctgtccagta acgtcctcaa cggcggcctg
     4381 agccaggccc agacgcggga agaactgagc cgggccatga gccagaccaa taccatgatc
     4441 tgccgtcatc tcggcgtccg cgatgaaaat acggtcctcc tctatattgc gccagacgaa
     4501 ttcaacgcta ctgtcgacgc cctgcagaaa gccgggaagt cgcagaaact gctccgcatc
     4561 atcgccgccg gcaacatcat gctcggcgaa ccggccctgg tccacgtcga aatgtatgac
     4621 aacaacctca tctatcaccg cggcgacgtc gtctacgaag aacggctgac agcccaggaa
     4681 ttgtcgggca atgccgaact gcaggtcatg cgtttcctcc ataacgtcaa tcagcagacc
     4741 caggccaaag tcctcctgcc ggacccgctg accggcaatg tcggggccct gacggcagtc
     4801 gaaatgttcg agaccatcaa caagataaag aaccacagcg gcgccgatat cgtcctcaag
     4861 gccgtcaccg aagccgatac ctacacggcc ggccccttgc agatcgacat ccgcgtagaa
     4921 gccgacgaat gatgggagct gttacatgaa ggcttctgtc atcatgaaaa gtccctttct
     4981 ctttatagtt tgtagtatag agtttgtagc aaatagtttt ataattactg caaactacaa
     5041 actgcaaact taaaactcaa aatgaggctg tctgaatgtg gcagcctcat tcctaggagg
     5101 agatacattg atctttgcca ttgaccctgg ttcggaaaag acggggactg ctgtcgtcgc
     5161 cgatgacggc agtctggtca cgaaggccat cgtgccgacg ccggaactgg gcgactattt
     5221 gagtcacatt tatgaaaact atcctttttc ccatatcgtc atgggcgatg ggaccaatca
     5281 taaaaagctg cagcccctgg tcgagcagtg gatcgccagg aaccacgggg atatcacttt
     5341 ttccctgaca gatgaaaaat tcaccactgt cgaagggcgc aagctctatt ttaaatatac
     5401 cccgcgcaag ggctggcgcc gtttcgtgcc actgtccttc caatatccgc cagaaccggt
     5461 cgatgacttc gtcgcctgga tcatcggcca ccgttattta gcgaaacaac gaggagaaaa
     5521 aatatgaaat acatcttgcg tgccctgggc ctggcccttg gcgctctcat ctacaccatc
     5581 ggcctggacg tcttcctggt cccgaaccac gttatcgacg gcggcgtcgt cggcattgcc
     5641 ctcatggcag cgcaggtgac aggcatcagt ttcagtgtgt tcatcatcat cctgaacatt
     5701 cctttcttta tctttggcta taagaagata ggtgcctttt tcaccatatc ttcgctcttt
     5761 tccatcctct gcctgtccgg gtggagcacg ttcatccatt acgaacgggt cacgtcggat
     5821 cctttcttgt cgaccattta cggcggcatc atcatcggcc tcggcgtggg cctcatcatc
     5881 cgctggggcg gttccctgga cgggacggaa gtcctggcca tcatcgccga caggcgcacg
     5941 cctttttccg tcggcgagac gatcatgttc ttcaacctct tcatcctggg cagttcgggc
     6001 tttgtctttt cctgggacag cgccatgtat tccctggtgg catacttcat cgcctataag
     6061 atgatcgacg tcgtcaccaa cggcctcgat gaaatgaagg ggatgctcat cgttacgggc
     6121 aagcacgacg aagtggccga ctgcctgacc agggatatgg gccgcgccgt gaccctcatc
     6181 aacggcgaag gcgcttacaa gcgggaaaag acgatgatcc tctactgcgt cgtgtcgcgc
     6241 ctggagatca ccaagatacg cgatgccatc accggcgtcg accccaacgc tttcgtatcg
     6301 gtcttcgata tcagtgaagc acatggcggc ctcttcaagc ggcataaaat gcatggataa
     6361 aaagtcaaaa aaagcttgac ataattggcg aatctgctgt attgacatac agctcatatt
     6421 tgctattata taaaataatt gggttatagc gttaacctga aaaaatgaca acacaggtga
     6481 tagaacacct attgggaggt atacaatggc acagttaaat ttcaatatcg aagacatttg
     6541 cggcactttg tcggaaaata aagacggctg gcgcaaagaa ctgacgtaca tcagctggaa
     6601 taatcgggca ccgaaattcg acctccgctc gtgggatccg gattatcagg ccatgaccaa
     6661 aggtattacc atgaccaagg aagaactgga aaaactgcgt gatgtattga acgaaatgga
     6721 tttcgataaa tactaaggag tgattaagta tgctttcggc aattcaatga gatgaatgga
     6781 atgaaagcat acttttttgt tttccttgcc aaatttgcca aaacattata taatctctag
     6841 ttgtatgtgc tgttagtact tggtaataat ttcaagggtg aatgaaatgg ttcgtatagc
     6901 gagagataag cgacatgctt tattaaagaa gcggattgaa gaaaatcctt ttataaacga
     6961 cgaagaattg gctgaagaat tttccgtcag cgtggctact atccgcttgg accgcatggc
     7021 cttgggaatc cccgaaatgc gtatccgcat ccgccagaag gcagaagcgg cgcagccgaa
     7081 actgcgtaac gcccctgtcg tcggggagct cgtggactgc atcatcggca agaatgccat
     7141 ttcaatcatg acggcaacag acgatatggt cgacgaagca cacatcgtgc gttcccagtg
     7201 cctatatgcc caggccagtt ccctggcgcg gacggtattg aatcttcctg tctgcattac
     7261 agccgtagcc aatattaaat ataaacagcc cgtcactgta ggcgataaac tgatcgccaa
     7321 ggccgaagtc atgcgccagc gcgaccagaa atactacgta tgggtcacga tccggaagat
     7381 gcggaaagaa gtattccggg caaaatttat catagaatct ttggaataat ggagcggtaa
     7441 tagaatgaaa atagctgtag atgctatggg cggtgattac gcacccgaag aaatcgtcct
     7501 cggtgccgta gaggccgtca aagcatatga ttttgatgtc gtcctggtag gtgacgaaaa
     7561 gaaaatacgc ggcgtactga gcaaatatgg cgccaaagaa agcgaccgcc tgtccatcgt
     7621 acacgccagc gaagtcatcg aaatgggcga acatccggct caggccatcc gccgcaagaa
     7681 agacgcatcc atcgtcgtag cgacgcggct ggtcaaagac ggcctctgtg atgctgtcgt
     7741 cgccgctggc agcactggcg ccgctgtgac ggcggctctc tttgggctgg gacgcatcaa
     7801 aggcatcgaa cggccctgca ttgcgacgcc tatcccgtcg aagaagggga tcacggtgct
     7861 cctcgattcc ggggctaatg ccaacagcaa gccgaaacac ctcgtcgaag gggccatcat
     7921 gggataccac tatgcaaaat acatcctggg cgtcgaaaaa ccgacggtcg gcctgctgaa
     7981 catcggcgag gaagcgtcga agggcaatga gctctgccag aagacgtatc cgatgctgga
     8041 aaatttgaaa actatctctt tctatggtaa cgtagaaggg agagatatac cgaaaggcac
     8101 ggtcgatgtc gtcgtctgtg acggcttcgt cggcaatgtc atcttgaaat ttggcgaagg
     8161 catggccatg ttcatcatcc gcctcgtcag agatacgatc aaacagagcg gcttcatcac
     8221 taaactgggg gccttagcgg tctacccggc cttgaaatcg ctgaagaaac gactggattt
     8281 caccgaatac ggcggtgcgc cgctgctcgg tgtcgatggc agctttatta tctgtcatgg
     8341 cagttcgaaa gccaaggcca tcaagaacgc cgtccgcgtt gccggtgaaa tcgccgaaca
     8401 ggacgtcgtc ggccatattc gtaaaagtat agaggaagaa gaggtagaac agtatgatag
     8461 tgaaagctaa tcccgctggg attcttggta caggtcactg tgtacctgat caggtatgga
     8521 cgaataaaga tttagaaaaa atgatggata catccgatga atggatccgc agccgtacag
     8581 gcatcggcgc ccgccacgtt gcgccagaag gcgtcacgac gtcggacctg gctaccaaag
     8641 ccgccgaaga agccttgaag aacgccggca tcaaagcgga agacctgaat ttgatcatcg
     8701 tctgcacctt gacgccggac cgggccttgc cgtcgacggc ctgcaaagta caggccaacc
     8761 tccatgcggt caatgccgct gcctttgatt tggaagcggc ctgctcgggc tttgcctacg
     8821 gttcgatcat tgcttcccaa ttcatcgaaa ataagatgta taaatatgtt ctggtcattg
     8881 gtgcggaaat cctgacccgc gtcgtcaact ggaaggaccg ctcgtcgtgt gtactcttcg
     8941 gcgatggcgc cggtgccgct gtcatcggcc ccgtaccgga aggctacggc atcctcggcg
     9001 gggctatggg agctgacgga acgggcggtg atttcctgac gattccggcc agcggtgtcg
     9061 aaatgccgcc gaccgatgcc ctgcgcgaaa acggcatggc ctatgccaag atggacggca
     9121 aggaagtcta taaattcgcc gtcaaagcca tgccccatgc cgcagaatta gccctcaagc
     9181 ggtcgggcct gacgaaggaa gacatcgatg tcctcgtacc gcatcaggct aatatccgca
     9241 tcatccagtc cgccgccaaa cggctccacg ttccgatgga aaaagtattc gtcaacatcg
     9301 agaaatacgc caacacgtcg ggggcttcca ttcccctggc tttagatgaa gccatcaaag
     9361 cagggatgat ccaccgcggt gatatcgtct gcctggatgg cttcggcgcc ggcctgacct
     9421 gggccagctt agttatgaaa tggtattgat ttttttacga gaggtgtgac tgatgatgaa
     9481 aaaggcattt gtttttcccg gccagggtgc ccagaaagtt gggatggtca aggatttata
     9541 cgacgcatat cccgttgtca aacagctttt tgaagaagcc gacgacgctt tgggtttctc
     9601 cctgaccaag ctctgctttg aaggtcccga tgaagaactc atgaagacct ataatacaca
     9661 gccggctatc ctgacggcca gcacggcctg ctgccgcgtc ctcgcccagg aaggactgac
     9721 accggacatc gctgccggcc acagtctcgg cgaatattcg gccctcgtcg ctgccggcgc
     9781 cctgaccttt gctgacgccg tccgcacggt ccgcctccgc ggccagttca tgcaggaagc
     9841 tgtacccctc ggtgaaggcg gcatggccgc tatcctcggc ctggctgaag ataagatcaa
     9901 agacatctgt gctcagattt cggctcaggg cggcacggtc caggccgtca acttcaactg
     9961 ccccggccag atcgtcatcg ccggcagtac caaagccgtc gaagaagctg ccgctgccat
    10021 gaaggaagcc ggtgccaaac gggctatcct ccttcacgtc agtgctcctt tccacagcac
    10081 gctcat
//
LOCUS       DAWABZ010000056        10042 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_1831_length_10042_cov_3.047261, whole genome shotgun sequence.
ACCESSION   DAWABZ010000056 DAWABZ010000000
VERSION     DAWABZ010000056.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 10042)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10042)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10042
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1831_length_10042_cov_3.047261"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4446..4519
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1936 a         3059 c         3136 g         1837 t
ORIGIN      
        1 gtcccgtaca gaccagggct ccgtctttca tgatgacgac ttcgtcggca tattgccagg
       61 cctgattcat gtcgtgcagg acgacgacga tggtcttctg ccagccctca ttgatgcggc
      121 gcaggaggtc gaggatgcgc agctggtggt gcacatcgag atacgtcgtc ggttcgtcca
      181 gcaagagtat gggcgcatcc tgggccagca agacggccag ccaggcccgc tgctgttcgc
      241 cgccagagag gtgctgcatg acctcgccag cataggcttc gaggccgacg gcctggatgg
      301 cccggtcgac ggcgtcccgg tcttcccggc tccggccgct ccagagacgg cggtaaggaa
      361 agcgccccat gccgactaag tcgcggacgg tcacatcagg cggcacgtca tgggtctggg
      421 tcaggaaggc catttcccgc gacaaggcct tacgggaaaa atcatggacc tcgcgctgac
      481 ccaggcggat agagccgctg gctgcgggaa tcatgcagtt cacggccttg aggaaggtgc
      541 tcttgccgca gccgttgggg ccgatgatgg ctgtcacctt ccctttgggc agggctacgc
      601 tgaaattatg gaggatggtc cgccgaccgc gccggacgac gaggtgctgg acggtaatgg
      661 catcagccat ggctcacact tccttccgca ggagaaataa gaaaaagggc gcccctataa
      721 aggccatgat gatgcctacg ggcaattcgg ccggtgccag gatgagccgg gccgccgtat
      781 cgctcaaggt cacagtcgcc acgccgaaga tggccgaacc agggatgatg tagcggtaat
      841 cggacccgac caggaggcgc acgatatgtg gcacgatgag gccgacgaag cccagcagac
      901 cggcaacgct gacagcgctg gcagccagca gggcggcgac ggccgtcagg cccaggcggg
      961 cgccttcgac agacaggccc aggctgcggg ccatatcatc gccgagctgg agcaggttga
     1021 gataataggc gccgacagct gataaagcca ggccgatgac cgtatacggt gcgatggccg
     1081 tcacgtcttt ccagctgctg gccgccaggc cgccgaccat ccacagcagg gctccgtgga
     1141 cgcggtcgct gtaaaagatg aggatgcccg aaatgatggc ccccagaaag gccgacacgg
     1201 cgacgccggc caggatgatg cggacaggca cgatgccgtc tttccaggcc aggaggtaaa
     1261 tgacggcggc tgcggccatg gcgccgatga aggctacagg cgtcatccag aagagataag
     1321 ccggccagac gatgaggacg acgataccgg ccaggccggc gccggctgaa atgccgatga
     1381 tatgcgggtc ggccagggga ttgcgcatga cggcctggag gatggcaccg gacaaggcca
     1441 ggttagcccc gaccagggct cctgtcagcg tccggggcag gcggatattc cagatgatct
     1501 gatccgccgt ccgggccgac ggctgccaga ggatctgcag gacatcggcc ggcgtaatgg
     1561 cggcaacgcc ctgcatgacc gacagcaggc agcctcccag ggccaggagg acaaagacag
     1621 ccagcatgag ccagcgccag cgggtccgat agcagccttc cctattcatg agccgactcc
     1681 ggatagacga gccgggccat ataggctacg gccttggggt agtccaggcc cggattgagg
     1741 aggaagtatt tttccggcag gacatagacc tggcccgcct tgacggccgt cagggcggcc
     1801 caggccggat tgccctggat atcttcgcgg atgcgttttt caatcttatc ggacgacccc
     1861 atggaggtca ggaaaatgac atccgggtcg gcctggacca ggctttccat actgtagggg
     1921 atgcgcgtcg tctggccgtc gccagcggca tcggcagcga tattcttcaa gtgcaggaga
     1981 tccgccatgt cgccggcaat actcgacggc aattcggccg atacggaact gggtgtcacg
     2041 tggaggatgg cgacccgttt cccctgggcc ggtacggcat cggtaacagc atggacggcc
     2101 tggtccagtt cgtcttcctt ggccttggct gcctcttctt tgccatagac cttgccgatg
     2161 acctcaaggt tgcgcttaac gtcgtcatag gtcttggtct tgaggaccag gaccgggatg
     2221 tggctctctt ccaactgggc cgccaatttt tcgtggtggt cagcgctggc aatgactaaa
     2281 tcgggctggc aggccaggac cttttccagg ctcacgttgt agacaggtcc gacgtccggc
     2341 acatgctgat atttttccgg cagcgatgca tcttcggctt tgaccgtcgc ccggccggcc
     2401 aggtcgccgt cgacggcagc ggcgaagttg aggagcgatg tcgacaagac gacgacgcgc
     2461 tgcggtttct gggccagagt gacgaccgtt ccccggtcat cggtcacgac ggcataggcc
     2521 ccggacgagc cgctgctttg gctagcgtcg ttctgaggcg cacagcccgt aagcaggacc
     2581 agacaaaaag cgacgatgag cagacaaatt tttttcaccg taaaggcacc tcctaaaact
     2641 ttctctttac gccgacctgc cagttgcgcc cggcagacgg ataattatgg tacaaatcat
     2701 aagcctcgtc gttgagattg ttgactttgg catacacggt cgtcgccgca tcaggcgaat
     2761 acgacgcgcc gacattccag acgacgtagg aattattgcg gtaatacgta tcgttgcgcc
     2821 cggtcccggc ggtcatatcg gcattggcct gccatttgcc gttgtgataa tggacaccgg
     2881 catgatagcc gttgggctga ttataggtcg tatcgaaggc caggccttcc cctttgtcca
     2941 ctttcgtgcg gatgtaggaa taacctgctt catagtccca tttatcgttg actttctgct
     3001 tccacgtcag ctcgaagcct cgcttgtctt cttcatcgag gttctctact tcgtggtatg
     3061 tcggatttga tcggtcccag cggatgacgt gctgcaagtt gctgtggaag atgctgcctt
     3121 cgatgttcgt cttgggacca gcctgatact ggaagcccaa agtctgggta tagccttctt
     3181 cgggctggag attttcgttg ccctgcgacg taatcccgcg ggacgtcgtc acatagaact
     3241 ggtcgttcag gcggggcggc gaaaagacgc ggctccacga ggcgtagacg ttgaaggcgt
     3301 cattggcctt gtaattcatg gccaggcgcg gcgtctggtg cgtcccaaaa gtgctattcc
     3361 ggtcccagcg cagacccggc gtgaccgtga atttgccgaa cgtcagggca tcttcggcat
     3421 agaaagcctg attatgacgt tttttgtcga tataatgggc ttcataattg gtccccatat
     3481 cttccgtcca ttcggcaccg gccgtcagat gctggtgcgg gccgagctgc cagttatccc
     3541 gccactccag gccctgggtg cgggtgaaat aatgattgga ccagtacgtc cgctggtaat
     3601 cgttgaaata gcgcaaaaag cctggctgag ccgtttcttt cttgaaatga taggtcacac
     3661 tgtagtcgtt gatcaggcgg tcgataggct tattggccgt caggtaccag cccgccgggt
     3721 cggctttata cgtcgaaccg tggccgtgat aatcttgatg ataggccttc acggtcaggg
     3781 acgtatggtc cgtcaggtcc tggtccagcc gcaaggataa ggacttcctg gcaatatcgc
     3841 cgcgggaatg gtccgtattg gctccgtgag gaaaggcata gtatgcccgc tgctccaggt
     3901 cgccgctgac gagccagctc gtcgcgccag cccggccgct gtgggtcagg gtatagccgt
     3961 gcatccccca ggaaccggtg taggcataga gggaagattc gcggcggtcc cccttcttgg
     4021 taacgatatt gatgacgccg ccgacagcgc cgctgccgta gcgcacggag ccgctgccct
     4081 tagtgacttc gatgtggtcg atagccccga caccgggcag gctggtcaaa tcgacgctgc
     4141 cccggccata gccggagccc tgggcattgg ccagactctg accgtcgacg aggatcagga
     4201 cccggtcgtc accattgagc ttgacataag aagagatgcc aggcaccatt tcattgacgg
     4261 tcacgccggt gacgcactgc aaggcctgct ggaccgtcgt atcgtgatgg ctttccatat
     4321 ccttggccgt aatgactgtc gtgttggact gattgggtgg gatggttggt gtaggtggtt
     4381 gcatctttgc ttccgataac tttacaggta gtttttcccg aatggcatcg aggacgtcgg
     4441 cgacgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnt cctttcccgg cccggcagcc cagccgcggc cgccggctgc
     4561 tgaacccgta tggccgctga gacgttctgt cccagcctgg gccgacggct gggagccctt
     4621 tgccgcaccc tttacagagg gctccccttt gccgctttcc gtcttggctg cataagtcat
     4681 cccgtcggca ccggcttcat ccggatcagg cgacgcaaca tcggaggctg ccacagccct
     4741 gtccgaagat tccggggatt ccggtccttc cggtccccta tcacggccat ccggctgaga
     4801 aacacggccc tcatccgtat cggtgccagc cgcgtcgtga ccgccggcac cggccaggtc
     4861 gtcggcgtta tagaccatga cgccggttac gccgtcctgg cgggtgtaat gatggatcag
     4921 cgtgaaaaaa ccgccagctg ccagggccag atagacggcg acgtggagcg atacggaaac
     4981 gccggcagcg acgcggcccc gtcgtttttc ccagggcttc atggctggcc tcccttgtcg
     5041 gcggccaggc cgatgtgggc gataccggcc cgcttgaagg catcgaggac gtcggcgacc
     5101 tgaccgtaat cgaggccccg gtcggcgcag aggacgacgc tgaaagcgct gtcctgacgc
     5161 tgctggtccc gagccgaagc caggagctca tcggccttga cgggctgtcc gttgagccac
     5221 agggacccat cggccttcaa ggtcacgacg tactgggccg acggctgggc ctgggcggcc
     5281 gaagcccgcg gcagctggac gccgaggctg tgcaaatcga ccatggacaa ggaggtcatc
     5341 atgaagaaga ccagcaaaaa gaacatgatg tcgatcatgg ggatgatcat aatcgacggc
     5401 tgttcaaaat cgcgaatatt acggaggcgc atggtcactg ccccgctttc acaggcatcg
     5461 tttccaatgc ggaaaaacac atttccatat ccgtaatgat gcggtccagg cgctgggtga
     5521 aataagaatg gacggccagg gccaggatgg cgacgcagag gccgaaagcc gtggcgatga
     5581 gcgcttcgcc gacgccgccg gtaatggcca cggcctggcc ggactggaca ttgaggatgc
     5641 tgaaagagcg gatcatgccg ctgatcgtcc ccaagaggcc caggagcggc gccatggtga
     5701 cgatgacgct cagatagtag agacggcggc gcagtttggc cacgaagatg cccgactgca
     5761 tttcgacgaa ggccttgacg tcaggacctg cctgatgctg aaagacatcc tgcaaaatga
     5821 ggggcaggcc gccgcggccg tccatggcca agcgctgggc ctggtcgtag cgccgcagct
     5881 tcatctgcgt gtagaattgt tcggcaaagg ctttcccggc gtccatgtct ccatagaaaa
     5941 tcgcccgttc gatgccaatg tagccgacga agagagaaca ggccaggagc aggtacatga
     6001 ccgacccgcc tgcctgaaat aactgaaaac cctgtaaaat cgcttccaaa ataccctcct
     6061 gtccggcctg ctgacgtgtc gaatgaaggt acaaaatcaa aaggagccgt cttgtagcga
     6121 cagctccctt acgtatcttt tttacaatct ccttcctatc gctcgcaggt ctatcggtga
     6181 ttatccgtca ggcagttctt ctggcttggc ttcctggcca tggctgcgcc ttcccatact
     6241 tcgtacagtg acatcgttgc agcctggctc caccctacag cggcgggacc gcgccggcat
     6301 tggaccggct tctctattga gtctatgaca cctgacctat ttcaaactta caaacataac
     6361 tatatcatgc ccagcgccag actgtcaatg aagggctgcc ccggcctttc cgtccggtga
     6421 gacctcgaca gtgacgacgc tgcccccttc gccccgttcc cagcggagga tctggcaggt
     6481 ccccagggga caggcagcgg gccagtcgtt gagccaggcc aggaggtcgg caaaggtccc
     6541 ggtcaggacc agcgtctgcc gactcccttc gcggacggac tgctgccacg acaagagctg
     6601 gacgttccgg gccgccaggg aggccgccac atcagggccg gcatcaggct ttgttgcctc
     6661 ccgcttctcc tggggccggg cccgatgggc atacggggcc agctgacggt ggacgccctg
     6721 ccagccctgc caggtgtgat acgatgagat ggcaaagcag gcggctatgg cagccagcac
     6781 ggcgaggacg ggaaaggccc gctgccagtc agcccttctt cgccaggcct tcatgacggc
     6841 tcacctccag ggaaaaaggg atcccctcgc cccggtcggg ctgcatcttg gccacggcgg
     6901 cctggcccat gccctgctgc tgcaaggtcc gctgccactg ctgaggccgg tccgcagcgg
     6961 acgcccggcc ttggatgacg acccggccgg cctgactgct cacggtttcc acgacgagcc
     7021 cgtcgggttt ggacgaagcc agggccacca tcaggtacgg cgtcagcagc gggccgtcgg
     7081 acggcccttt cttggcgcgg tcggccaggg cctggctgcg ccggatggca tcattgcgct
     7141 gctgtatctg ctgctgcacg ggatagagat agtctgtata ataggccttt tcccgttgga
     7201 tctgatactg aaaaaagagg cctgccccgg cacagacagc cagcatcagc cccagggcca
     7261 gacaggccgg ccgctgccag cggcgatggt cctggcggta gctggccggt acgaggttgc
     7321 atttcatgat tctccctcct tacaagaaag ccagcatggc cgtaggctgg gacacgtggt
     7381 aagcatccat caggcggcgc acggcttcgc tcggcccggc aggctgccat tccgtctcgt
     7441 ctgccccatc cgggagccag cggaagaccg gcccatcagc cgtcggcaca gccgcctgta
     7501 cgtcataggc caggggcagg ccgtcgcgca ggcggacgcg gtgctgctgg ccatcggggg
     7561 cttcctgaaa ggccagggtc cccgtaccgg ttggccaaag gcgccccaaa aaagcaggca
     7621 gcaagtcgat ggactggacg acggctccgg cagcgccggc agcccgacag atagcggcga
     7681 cacattcggc cggataggcg ccgaccgtcc agtcaccgct cccgtcaccg cctttcgacg
     7741 gacaagccgc ctcgaagtca taaggccgcc cttcttcact gaccaggccg gagggcatcg
     7801 gcttcctgcc ggtctgtcgt ccccagggac aggcgcttcc aaaggacgct cggcccgccg
     7861 atgaggacgc gcaggcgcca gccagccagg ccatagcgct gcaggagttc tggcagacag
     7921 gactggacgc ccccttcctg gcccatccag tccgggtccc gcttggtttc atagtcgcga
     7981 tgaccctggc ggaaggccac gcgtccatac caggtccgtt tcgtcttcaa aagatggaca
     8041 gactgtccat tcagccataa acaggcattt tgttgccacc acataagctc acctctctac
     8101 taattgacat actgtacgaa atgcagcacg gtccggccct gttcatcggc cgtcacggca
     8161 aagtgcaggc cgcacgtctg ctggacgccc gttcccggcg cagcggcccg ggcctgcaga
     8221 tagccgcccg gcgtctgacc ggccggctga tactggcagc ggacggtcag gatgtcccca
     8281 gggccagcca gcccttctgc ccggggcacg gccatggcct ggcccgccag aaaagcgtcc
     8341 gggtcctgcc gcagctggtt ccagccctca cagagggcgc tttcggccaa ataggtcagc
     8401 cgcacggtct cttcataggt ccgggccatc tggaagtgct gccacgccag ggtcgccgcc
     8461 gtcagggcca ggctcagggt cagtacggcg acggtcacga tagaaaaaga aaagaagcca
     8521 ttgcgtcgtt tcatgattca ccttcattct ggggacacac ggcaaactgc acgggccggg
     8581 acaggccgct ccgccgttct tttaggagaa aggcgacttg tacggcctgg ccggtccggg
     8641 agaaaaaggg ccgaccgtcc aggggctgga tgaccagggc ccggccctgg acggtgctgc
     8701 cgccgcctgt caggggctga tagttgccat cgctgaggcg gacatacagg ccttgatggc
     8761 ggacggcaaa gcccttcacg gcgcccttgg ccgtgcggaa gcggacctcc tgaggacgga
     8821 cttcaacgtc ccggccatag cgcagggcct ggaacagcgt ttcttctacg ctgacgtgct
     8881 gccgcagcag gccttcttca atctggcagc gccaatccag gcgggccgtc tgtcccgcca
     8941 gaggcagcaa agccgtcaga gtcagggccg acaggctcaa ggccaggagt acgtccaaga
     9001 ggatccagcc gtttttcttc ggtccctgcc ggcaccagaa gccggcagga aacatggggg
     9061 ccatggtcat cacctgcctc tacctgccaa aggacatagc cctcgccgag agccgtctgc
     9121 gcatgcgtca cctcataggt ccggccctgg atgacgacgc ggtctgatgg cggacagccg
     9181 ctcttccagc cttccatgac ctgccggcag accatggctg ccagctgccg ctgtctggcc
     9241 tggtcatgca gtctgacggc ctggagggaa aggcccaggg ccgccgtgaa caagcaggct
     9301 ccgaggagca ggcccgccag gccgtcggtc aggaaaaaga agccattggc ctcaggcgat
     9361 acggatgcgc ccggtctgcg ccgccacgac gatagaacgc gtataggccc catcgggcga
     9421 agtaatgacc acagccatcg tatgctgcgg acgaccatcg acagcaaagt gtacatctcc
     9481 tttcgttacc tgcaggcccg gcggataggg ccgttctttg acccggatgt agccttcctg
     9541 gaggacgtag cgcttttctt ccaggaccag tttccagcgg ccgttgctct ggttgttgcc
     9601 gacacagcgc tggcggacct gctgcatatc cagggccatt tcccgcgcca ggccatcgac
     9661 ggcctggtgg ctgcggctgc gctgaccggc agtgagtccc aggcccagat acaagagcag
     9721 caggcccagg gccagcatca ggccggtcag gctgataaag ccgttccgcc tcaccatagc
     9781 cagcctccgg gccaggcgcg ctggagcggt tcctgggcca gccaccagcc ggccaggagg
     9841 aacgggccga agggaatgat cgtcttccct gtgcggaaac ggtagaacaa ggccacgagc
     9901 gcggccaggc caaaagaagt gactgccatg gtccaggccc cccagcccgg gcagcccggc
     9961 gccagggcca gggccagctt gatgtcgccg ctgccgatgg acccgggcca gagaccatag
    10021 aggagcagga agagggcggc ga
//
LOCUS       DAWABZ010000057        10009 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_1837_length_10009_cov_3.961021, whole genome shotgun sequence.
ACCESSION   DAWABZ010000057 DAWABZ010000000
VERSION     DAWABZ010000057.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 10009)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10009)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10009
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1837_length_10009_cov_3.961021"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2247 a         2969 c         2830 g         1963 t
ORIGIN      
        1 tggcaagttc agctttggca aatccgtagc tgacaatggc tggaatatgt tcacgaacat
       61 gctggaatat aagctggctt ggcaaggcaa aaagcttatc aagatagaca agtggtaccc
      121 cagcagccag ctgtgccatg tttgcggata tcagaaccac gaaaccaagg acttgtctgt
      181 aagggaatgg gattgcccta aatgtggcag tcatcataac cgagataaaa acgcagccat
      241 aaatattcga gaagaagcca ggcggatatc tgcctgacgt aatccataaa ataccgtggg
      301 tcgcacggga atctacgcct gtggagagag tgtaagccgc cgcaactctt tggagtccgt
      361 ggcgctgttc tcgttgaagc aggaagctcc cgcctctata ggcgggagta cgttcactat
      421 atttattgca atgaatttgt atagatggga gattggggtt atggcgcaag aagcaaatgt
      481 attgatgggg ttgagtgtca aagcgatgat ttttcatgaa gggaagctgc tgctgttgca
      541 gaagaacgat gccgaagggc tgcatcattg ggaattccct ggcggcggcc tgcgctttac
      601 agaagatttt gaagccggcc tgcgccggga agtcagcgaa gaaacgggcc tttccatcac
      661 cttagaggct ccggcaggta tctggagtta ccagaagaaa gacggccaat tcctgaatgg
      721 tgtcattttt acggccatga ccacgtccgg taacgttaca ttatcagaag aacatctggc
      781 ctaccgctgg gtcacgccgg ccgaactgcc gtcgtaccgc ctgcacggct ctctgcagcg
      841 ctcgctgaag cagatgaaac agttctcgta tgaaaagagc cacgctttgc tgcgcggttt
      901 cctttcggct atatgaggtg gcccatggat agagaaaagc agcaaaaggc gcctttcgtc
      961 gaagcactgg aagggtattg ccagcagcac atgaaggcct accatacacc gggtcataaa
     1021 ctgggacagg gcatttctgc ctaccaggcc gatctctttg gcgacgccct gcggcgggac
     1081 ctgggcgtca tgtatgcttt ggatgacttg ttccagcctg aaggggcgct gaaggaagcc
     1141 atggaactgg ccgccgacct ctatggcgcc gggcggactt tcttttccat caacggcacg
     1201 acggcctgca tcgaagccat gatcctggct gtctgcggcg atggcgatga gctcatcatc
     1261 ccccgcgaag cccacaagag cgtcatcaac ggcctggtcc tgagcggcgc cgttccggtc
     1321 tatatggaaa gccgcttcga tgcggcccgt caggtatcat tgggaccgga cctggacagt
     1381 ctgaagcggg ctgtcgccgc ccatccccgg gccaaggccg tcgtcttcac ctatccgact
     1441 tacgacggca tcgccggtaa cttgaaggcc ctcacggcct atgcccatga aaaaggcctc
     1501 tatgtcctcg tcgatgaagc ccatggggcc catctgggct tccatcggga cctgcctgcc
     1561 gaagccctgg cctgcggtgc cgactgcgtg gcccagagta cccataaaat ggccggctcc
     1621 ctgacgcaga cgtcgatgct gcactgtcgc aaaggctttc ccctgacgga ccgcgtcgcc
     1681 gcctccatgg ccgtcgtcca gtcgacgagt cctcattact ggttcctggc ctccctggac
     1741 agcgcccgcc agcagctggc cctggaaggg ccggaactca tcggccgggc cctggacctg
     1801 gcccggtcgg cgcgggacgc tatcaacgcc attcccggct tgaccgtctt tggccgggat
     1861 atcatcgacg gacaggtcgt cagcggcttc gatgaaacga agattactat cgaattttcc
     1921 ggcgtcggcc tcgacggcgt gacggccgaa cggctcctgc gtcagcaggg catcgaagtc
     1981 gagctcgtcg ccggcaatca cgtcctggcc ctgatcacct taggcgatac ggaagaaaca
     2041 gcccaggctt tggtacaggc ctgccggact atcgccgaag ggcgcagcct ctgccaggcc
     2101 ggcacggtgg ccgaacaggc cctgcccgtg ccccgcgtcg tcatggcgcc gcgccaggcc
     2161 tggaactgcc cggtagagac ggtgcccttt gaaaaggcct gtggccgcat tgccgcagag
     2221 aacgtgactt tctacccgcc aggcattccg gtcctgacct tgggtgaaga aattacggcg
     2281 gcctgccagg cctatatccg acgcaagatg gctggcggct ataagccgaa cggcccggct
     2341 gacgggacct tggcgacgat acgcgtcttg aaggaggatt ttcatgaaag gtaaattatt
     2401 catcatcgaa ggcggtgacg gcagcggcaa agcgacgcag acccgcctgc tgacggaaca
     2461 cttgcagcgc gacggctatg acgtgaagtc cgtcagctac cccgactatg acagcgactc
     2521 cagcgccctg gtcaagatgt acctgcgcgg cgacttcggc agcgatgctg atgccgtcaa
     2581 tccctatgcg gcgtcggcct tcttcgccgt cgaccgcttc gcgtcgtatc agatgaagtg
     2641 gaaagccttc tacgaacagg gcggcatcgt cattgccgac cgctatacga cgagcaacat
     2701 gctctatcag atgatcaaga tcgacgaccc ggcccagcgc cgggcttatc tggcctggct
     2761 cgaggacttt gaattccgca agatgggcct gccggcaccg gacaaggtct tcctcctcga
     2821 cgtccccctg gctgtcacgg agcggctcat ggcggaccgg acgggcaaga ccggcggcca
     2881 gaccggcgat atccacgaaa agaatgaagg cttcctggcc aaatgccacg ccgcttatga
     2941 tgagctggcc caacgctatc actggcagcg cattgcctgc acggaaggcg gggccatgcg
     3001 cacgcctgaa gccatccatc aagacttata tggctgccta tttactttgt aggggatttt
     3061 ttctataatt aattgttaag gtattttatt ggcgtaaggg ggcgacatga tggacaggac
     3121 actgtttgat ccgattatcg gccacagccg cctgaaagaa cagttcgccc gcctcatcga
     3181 cgaagaccgc atccctcatg ccatgatttt tgccggcccg gctggtatcg gcaagaccct
     3241 gatggccatc gccgtcgcct cggccctggt cggacgccgt gtctttcccg acctggccag
     3301 ccgcgaaggc gaagccgtag ccggcgataa agacgatgcc ttctatctgg cgccgatggg
     3361 ggccatgctg aaagtggacc agttccgcca gctccagggc gaactggccc tgcagggcga
     3421 aaccggccgc cgccgggtct gtatcatcga ccatgtggag acgatgaata cagaatttgc
     3481 caaccgcatg ctgaagattt tagaagaacc gccgtcgggc gtctgcttca tcttgattac
     3541 ggaccagccc gatttgctgc tgccgaccat catttcccgc tgtgcccgct ttaccttcga
     3601 ccccgtcggc gacgacgaaa tgcgccaggg actgcggcgc ctgcggggcg acggcgggaa
     3661 ttgggacgaa gccatcctct ggggtggcgg catcgtccgg actgtattgt cctatctcga
     3721 cggccagggg acggataagg cccatttcgc cctggatttt ttgacgacta cggcgaaaca
     3781 tgcctgtccc tatgccaaat ggctggccct gtccgcggcg ctgacggata gggaaacgac
     3841 ggagatactc ggctggatca gcctttttct ccgcgacatg gccgtcctgc gcagcggtgc
     3901 cggccgggac tatatacgtt taaaaggata tatccatgaa atgacagaat tactgccgta
     3961 ttggacggat gacgccgtct ttggcctttc ccgggtgctc gatgaagggc tggaagccgt
     4021 ctcgcgtcac gtcaacgtcc gcctggtctg ggactatgta tgcctccagg ccatacggct
     4081 tagaggagga attcaatgat cgttgtaggt gtacgcttta aacctgccgg aaagatatat
     4141 tatttcgatc ccgccactat cgaactcaag ctggatgacg gcgtcatcgt cgaaacggcg
     4201 cggggcatgg aattcggcca tgtcgtcatt gcgccgcgca gtgccgattc gtcggaagtc
     4261 gtccagccgc tgaagccgat cattcgcaag gcgacggcca aggacattcg ccaggtggaa
     4321 aagaacaagg aacgggaaaa gaaagctttc gccatctgcc tggcgaagat cgccaagcac
     4381 aaactgccca tgaaactcat cgacgtcgac tataccttcg acatgggcaa gattatcttc
     4441 ttctttacgg ctgacggccg catcgacttc cgcgaactcg tccgcgacct ggcgtcggtc
     4501 ttccgcacgc gcatcgaact gcgccagatc ggcctgcgcg acgaagccaa gatgatcggc
     4561 ggcatcggct gctgcggccg gcccctgtgc tgctctacgt tcctgggcga tttcaagccc
     4621 gtttccatcc gcatggccaa gggccagggc atgtccctca atccgaccaa gatttccggg
     4681 atctgcggcc gcctcatgtg ctgcctgcgc tatgaaaacc agctctatac gtcgggcgag
     4741 ctgaagtgct gcaactgcca gcagaagagc tgtgaacagc agaagccgcc ggctgtgggc
     4801 aaacgggtca tgaccgacga aggtgtcggc acggtcctgc gcgtccacat gaaggaccat
     4861 acggtcaaag tccagctcga atcgggacac aacatcaacg tgccctggtc ggacataggg
     4921 gaaacggaac atgaacattg agttaggccc gcacgaacgg ctggacgacc tggtcctgga
     4981 cggcatgaaa gtcattcagc gcgatgacca gttctgcttt tctctggata cggtccttct
     5041 ggcccacttc ggtgccgtgc ctcacggacc ggctttggac ctggggacgg ggacggcggc
     5101 tatccctctc atcctgacgg cccgcggtgc caaggcgctg acggcagtgg aattcaatcc
     5161 cgtcatggcc gacattgccc gccgtaacgt cgccctcaat ggccgggacg accgcattac
     5221 catcgtcgaa ggcgactacc gccgcatccg ggagtgggcg gcgagcggcg cttttgccgc
     5281 agtctacgcc aacccgccgt accgggaaaa gagccgcggt gcctatagcg acgtcgacgg
     5341 catccgccgg gcctgccatg aagagacggc gacccttggc gacgtcctcg atgctgtaaa
     5401 atacgccttg aaatatcacg gccgcttccg catggttcat attacggaac gactggccga
     5461 tattttagag gccatgcgcg cccgagatat cgaaccgaaa gtattgcaga tggtccacgg
     5521 ccggcctgac aagaaggcca agctcttcct cgtcgagggc atccgcggcg gcagcaaggg
     5581 catggacgtc ctgccgccgc tcatcgtcca caaccccgac ggcagctaca gcgatgccgt
     5641 cctgcgatta tacgggaaac gataactagg gaggaacatg atgccggaaa tctttcagaa
     5701 agggacgctg tatctttgcc cgacgcctat cggcaatctc gaagacatta cgtaccgcac
     5761 ggtccgctgt ctccgcgaag ccgacctcat cgcggcagaa gatacgcgtc atacgaaaca
     5821 gctgctcctg gcgtatgata tcgatacgcc gttgaccagc tatcatgaac acaataaagc
     5881 tgaaaaaggg ccgcagctca ttgaaaaatt gaaagacggc ctgatgatcg ccctggtcag
     5941 cgatgccggc atgccggcca tctgcgaccc gggcagcgat atggtccgcc tggccctgga
     6001 agccgatatc cccatcgtac ccctgcccgg cgccaatgcc ggcctgaccg ggctcattgc
     6061 ctccggtatg gatacgacgc gctttacctt tgtcggcttc ctgccgaaga cccagaaaca
     6121 ccgcctgccc gtcctggaat cggtgaaagc ctatgaaggg acgctcatct tttacgaagc
     6181 gcctcaccgc atccagcagg tcctgggcga aatgctcgaa gtcttgggcg accgccgggc
     6241 cgtcctctgc cgggaactga ccaagcggta tgaacagtac ctgcgcggca gtctcagcca
     6301 gctgcgggcc gacctggctg agcaggggac gcgcggtgaa ttcgtcatcc tcgtcgaagg
     6361 ggcggcgcct gctgccgaat ccgctgcctc aggcgacgtc gattacgcgt ccctggtccg
     6421 ggaactgatg gaacagggcg tcgataagaa agaggccatc cggaccgtcg ccagacggtg
     6481 cggcgtgcct aaacgtagtg tataccaggc tgcgcttagt ttataacatc cggctcattt
     6541 gccggtatag gaggaaaatc ccatgtctga aaaaagaaag tattacatta caacacctat
     6601 ttactatcca agtgccaaac ttcacatcgg gcacacgtac tgcacgacta ttgccgactc
     6661 gctggcacgg ttccaccgtc tgcgcggcga cgacgtcttt ttcctgaccg gctcggatga
     6721 acacggccag aaaatccagc gtgcagccga agcaaaaggc gtcaaaccga ttgaatacgt
     6781 cgacgaaatc gtcgccctct tccagcagct ctggaaaaaa ctcaacattt ccaatgatga
     6841 cttcatccgc acgacagaaa aacgccatga aaaagtcgtc caggctttga tgcagaagtc
     6901 ctacgacaac ggcgatatct ataaaggtga atacaaaggc tggtactgta cgccgtgcga
     6961 aacgttctgg ccggaaaaca agctgcccga aggcaaacac gtctgcccgg actgcggccg
     7021 tccgctggaa ctcgtttcgg aagaagcgta cttcctgaag atgaacaaat acgccgaccg
     7081 ctggctgaaa ttcatcgacg aaaacccgga cttcatccag ccggaatcgc ggcgcaacga
     7141 aatgatttcc ttcgtcaaga gcggcctgga agacctctgc gtttcccgta cgtccttcga
     7201 ctggggcatc aaggtcccct gggacccgaa acacgtcgtc tacgtctggt tcgacgccct
     7261 ggtcaactac gtcacggctg ccggctatct cgatgacccg gaaaaattca agaaattctg
     7321 gcccgctgac ctgcatctgg tcggcaagga aatcgtccgc ttccacagca tcatctggcc
     7381 gatcatgctc atgagctgcg gcctgccgct gccgaagaaa gtcttcggtc atggctggct
     7441 catcgttgac ggcacgaaga tgagtaaatc tttgggtaac gtcgtcgacc cgatccccct
     7501 catcgaccgc tacggcgccg atgcgctccg ttactacctc ctgaaggaaa tccgcctcgg
     7561 ccaggacggc aacttctccc tgccgtcgtt catcaaccgc atcaactccg atttggccaa
     7621 cgacttgggc aacctcttgc agcgtaccct ggctatggtc gaaaaatacg aaggcggcgt
     7681 cgctgccggc ccgacggtca aagaaccggt cgacgacgat ttggcaacgt gcgctgtgga
     7741 aacggcaaaa cgttttgaaa cctacatgga cgacatgaag atcaacgatg ccatcaacga
     7801 cgtctgggcc ctcatccgcc gcagcaataa atacatcgac gaaacgacgc cgtgggtcct
     7861 ggccaaagac gatgccaaag cccagcgcct gcagacggtc ttgtacaatc tcctcgaagc
     7921 ccagcgcatc atcgctaccc tggtcagccc cttcatcccg gccagcgcag cggaaatgtg
     7981 gaaacagctc ggcttaggtg actttgccga agccaacctg ccggctgccc aggaatgggg
     8041 ccgttatccg gctgatacga aggtctgcaa aggccacgcc ctcttcccgc gctacgattt
     8101 gaaggaagaa gtcgccgaag cggaagaaga aaagaaagcc gctcaggcct gcccggctgc
     8161 ccaggaaccg acgaagccgg aaatcactat cgacgatttc agcaagatcg acctccgcgt
     8221 cggcaaaatc gtagcctgtg aaaaagtacc taaagccaag aaactgttga aactgcaagt
     8281 cgacctcggt acggaaacgc gccagatcgt cagcggtatt tccctgtact ataaaccgga
     8341 agacctcatc ggccactgcg tcatcgtcgt caccaacctg aaaccggtca agctctgcgg
     8401 tgttgaatcg aaaggcatgc tcctggctgc ttccgatggc aacgataacc tgcaggtcgc
     8461 tttcgtcgac ggcatggcac cgggaagccg ggtccgctga tgctcttcga tacccattgc
     8521 cacgtcaatg acggtgctta cgctgaagac catccggccg tgttcgaccg ggcggccgca
     8581 gccggcgtag gcctcatgat ctgcccggcg acggatatgg aaacgtcagc ccagtgcatc
     8641 gccatggcca gggaacacga cggtctttat gccgccgtcg gcatccatcc cgaagaagcg
     8701 gccaaggccc aggacggcga tgtcgacaag ctgcgccaat ggatacagtc ggaagagaaa
     8761 gtcgtcgccg tcggcgaagt cggcctggat tatcactggc ctgaaccggc ccgggatgtc
     8821 cagcaggccg tcttcatcga catggtcaaa ctggctgtgg aactcgacgt acccatcatc
     8881 atccatgacc gcgaagccca cggcgacacg ctggacatcc tgcgccagta cggcaagggc
     8941 atccgcggcg tcttccactg ctattcaggc agtctggaaa tgacggacga actgctgcgc
     9001 atgggcttct atttcggctt tgacgggccc ttgacctatc ccaattccaa acgggccaaa
     9061 cgcgtcgcgt cccatttgcc gttagaccgc atgctcatcg aaacggactg cccgtacttg
     9121 acgccccagg cccaccgcgg ccagcgcaat gaaccggcct atgtccacta cgtcgccgaa
     9181 gaaatcgccg ccctgcgggg catagacgcc gccgaagtgg cccgggcgac cacagaaaac
     9241 gccaagcggc tgtttgcgat tccgtaacct gcaatcttgt aggggcttgc acgtggcaag
     9301 cccgctccgg aatatgagcg attttccagc ttggagagag cgtcacccaa tgcccaacgg
     9361 gtcgcccacg tggcgacccc tacagtgtga aacagagttg ccgcacgaaa gccgatagct
     9421 taatgcgata atttctgctt acttatccac aaaggagaac accatgtcac gaggaaaacg
     9481 tcccgctatc cagcttacga ttacggggaa actggggact atgggctgcc atcgcggcca
     9541 tcacatcggg gaaacctttg attacgatac ggaccgggga aaaatctgtc ccatggccct
     9601 gcactgtgcc tttccctatg tcgatatcct gcgctatggc ggccagcttc cgggacagcc
     9661 ggaaggagaa gccgaattct gctgttccga cgccgacgta gccctggtct tcaaggctaa
     9721 aatcattagc gattagcagg taggagttag ggggcgcacg aaggcacatg ccattatgcg
     9781 aggccccttt ctctttatag tttgtagtac ggagtttgta gtttgtagaa gatggcttta
     9841 aatttaaaag cctttactaa aaactacaaa ctgcaaactt aatactcaaa aaatcccttg
     9901 acagaatcct tcggatttgg tattataatc aagtcttctg aaacagacaa cacacatgcg
     9961 gaagtagctc agtggtagag catcaccttg ccaaggtggg ggtcgcgag
//
LOCUS       DAWABZ010000058         9974 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_1843_length_9974_cov_3.266458, whole genome shotgun sequence.
ACCESSION   DAWABZ010000058 DAWABZ010000000
VERSION     DAWABZ010000058.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 9974)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9974)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9974
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1843_length_9974_cov_3.266458"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3368..3377
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2224 a         2854 c         2978 g         1908 t
ORIGIN      
        1 actttatagt ttgtagtaca gagtttgtag tttgcagaga aaacctttaa atttaaaccc
       61 atccgctaac cactaacaac tagccactag ccacttaaaa agcgctgtct tcatgcgaca
      121 gcgctttctt tttttttgca atcagcggtc tgcggattgc gacaaacgac gaacgaccta
      181 cgcttctgtt tctccgatga cgacgcggcg gccgccctgg gcttcgtaga ggcgatggga
      241 gatggagagg accgtccggt ctttggcggc ggcctgcagg gcctgcagga cctggctttc
      301 tgtcagggaa tcgagattgg ccgtgatttc atcgaacaag agaatctgcg ggttcatgac
      361 gacggcccgg gcgatggaca ggagctggct ctggccgcgg gaaaagagtt ccggccggaa
      421 gggcgtgtcc agtccctggg gcagggcaca gcacgtatcc cacagaccga cgaggtgcag
      481 ggctttttcc atggcgcctg ctgtgacccg cgggtccttg aggctgattt ggtcggcgac
      541 ggtaccgctg atggcgtgga actgctgctc gacgtagccg aagacctggc gccggacagc
      601 aggggccagc cgggacgggt cgcagccaaa gatgcggacc tggccttccc ggggctggta
      661 gaggccgagg atgagtttga agagggtgct cttgccggcg ccggtacggc cggtgatggt
      721 gaccatttcc ccggcggcga cggtgagcga aaagtggcgg aaaatatctt tttcccggcc
      781 atagccaaag gcggcatcgt cgatgaccag tggtgccgct gctgcgtcag ggacttccgg
      841 ttcttttcgt tcctggatgg gttcggcaaa gaagtccttc aggcggccga taccggcgac
      901 ggcagactgg atgttctgga tttccatgcc gatgctttct aagggcgaga agatgcggcg
      961 cacataggcg atgagggcga cgacgctgcc gacggtcatg ccgaagattt cctgcaggga
     1021 cggactgctg acggacaggg ccatcatcag gcagatgatg accgtgcagc tggtgatgat
     1081 gatcggcgaa tagatggagt cacagaaatt gctccgttcc atggcgtcga aactggcccg
     1141 gatcgtccgg ccatagcgct gtcgcaggta gcgttcctgc tggcacgtgt gaatcgtgcg
     1201 gatattatgg accgtttccg gaatgagttc gttcgtccgg cctacggcga tgcggttgtc
     1261 ccgctgggcc tggagcatgc gcttctggaa atagcgggtc aggataaaca agaggggcag
     1321 gcagatacag agcaatatgc ccaggcccag actcaggtgg aagacgacga cgaggatgct
     1381 gagaatcgtg aaggcatcgg caatcatgct gacgacgccg gaatcgaaga ggttttccag
     1441 ggtatcgacg tcgttgacga agagcgatgt gacagcgccg gcttcatggc tgacgaagta
     1501 cgccgccggc aattcgtcga ggcgctggca catggctgag cgcaggccgt gggtgatttt
     1561 ctggccgaag acggtgatca gcgtctcctt gagggcatcg acgaggccgc tgccggcatt
     1621 gagggcaaag tagaggatgc ctgccgtcag caggggatag gcgctgaacg agccgctgac
     1681 caggctgtcg acgatgtatt gcaggatgag gggcggcgtg acgcccacgg cgatggacgc
     1741 gatgatggct acgatgagga tcaccaggag caggccgttc tttttcaggg tctgccagaa
     1801 aatattacgc atggtgggca cctccctggg catgctgcag gtcatagaga ttccggtagg
     1861 ccggacagga tgcatataga gcttcgtgcg tggcgacgac gctggtcccg tccccgttga
     1921 gccagatgac ctggtccaga ttggggaaga ggctcaggcg gtgcgagatg aggaggatga
     1981 tgcggtcgct gtaatcctgg cgcaggctgg tgaaaatctt ccgttctgtc gccatgtcga
     2041 cggcggcaaa agggtcgtcg aggatgagga gcggccgcgg atggaacagg gaccgggcca
     2101 gggcgatacg ggcctgctgg ccgccggaca agcgctggcc gccttcgccg atgggcgtct
     2161 ctatccccat cggaaaggta gcgcagtcct catccatgca gacctttttc aggaccgggg
     2221 cgatgtcacc ggacaggccg aggcggatgt tctcttcgac ggtagccgca aagagttccg
     2281 cgtcgtggcc gcagtagccg acgatgccgt ctggcgccgt gtggccgttg cccagttcct
     2341 tggcgccaaa gaaaatctgg ccgccgtgcg ggttttcaca gaggaaggcc tggcccagcg
     2401 tcgatttgcc gcaggcgaca ggcccggtga tgccgatgat agtaccggga cgggccgaga
     2461 aggagaggtc ctggaaaata gggccgtcac tgccgggata ggtgaaggac aggtggcgga
     2521 ccgtcagggc tgccggggct gccggcggta cgggatcggg cagttccgtt gccgcctgga
     2581 gatggggctg gatgcgctgc cacgaaacct gggctttctg gatggcgttg aacaattttg
     2641 ccgcatggga tgatttcgtc gccagcttga tgaaacaggc caggtacgtc gagaaagcgg
     2701 cgatatccca gctttcccaa cccgttccca ggacgttgcg gccgccgaac cagatgacca
     2761 gcatcgtgcc gatcatggcg atgactttgt aaatcggctg gagtgtattt tcccagagat
     2821 tggccaggat tatcttcttt tcatagtcag ccaggtagcc ttcataccgc tggtcctgta
     2881 tggcttcttc gccgtagacg cggtacgtca gggcgttgct gatgcggtcc agggtcatgc
     2941 ccgacagggc gccgctgctt tctttgctgc gggccgccgt cacggcgacc aggcggcgca
     3001 ggctgttggc cagggcatag gcgatgggcg ggaagacgag gacgcacagc gtcaggcgcc
     3061 agtcgtacca gacgagcatg accacatagg ccgccatgac gacgccggta tcgaagattt
     3121 ccgtcgtgaa cttgcgcatc ccttcgacgc aggtgtcgat gtcggaaatg actttggtca
     3181 tcaaggcacc cgtatcggcc tgctccatgt cccgcttcgg cgtctggacc aggtgctggt
     3241 aaagccggtc tttcatggaa aggctgatgt tgttggcgaa cttccggacg tacaggcgct
     3301 tgacatagcg ggcgccctgg acgccgagga tgaccagggc gtaacagaga cagagggcgt
     3361 acatatcnnn nnnnnnnccg ggtaccgccg aaaatatcgt agaggtattg ggccagctgc
     3421 ccgtcgaacc aggggccggc aatcatgccg acattgtata agataccgct cagcgtgacg
     3481 atgagcagca atttcttttc atggtaaaaa taataacgga ccgaacccgg gccgaatcgt
     3541 ttcaatggga acacctcaca tttgatgatt atagttcata tatcatacac tattatgggc
     3601 ctttgtacaa caggacgcag gcagggtgcc gttcatcgtt cgtcgtagga ctatcgcatg
     3661 aagatttctg ccatcatgca aggtaccttt caatttatag tttgtagcat ggagtttgta
     3721 gaaaaccact cactaaaaac tacaaactgc aaacttaaag ctcaaaaagg cgttgttttc
     3781 atgtaacagc gccttttttt cgaatgagac tattgcctat atgatgggaa atggggacag
     3841 gcctcttgcg taaagtcaaa aagacgaata taatagagtt gtatgaaagt caaaaaggaa
     3901 aatatatttt tacggggtga ttatgatgag tgtattagcg gataagattg aacaattcat
     3961 tttacataaa ttgttagaag aagacgattc agacattgta ttgcgccgta atgagctggc
     4021 cgacgaactg caatgcgctc cgtcccagat cagctacgtc ctgagcacgc gtttttccaa
     4081 cgacaagggc tatctggtcg aatcgcggcg cgggtccggt ggattcgtcc gcatcatcaa
     4141 gctgacgccg aagaagcagg accgcacgcc ggtcctcctg cacgaacggc tgccggccgt
     4201 cagcatttcc atggaagacc tggatgccct gctgtaccag ctcctcaagc ggcgcagcct
     4261 gaccaacaac gaagcggtca tcctacactt gacttttgaa tccctctatc gccgcatcga
     4321 cgatgaaggg acgcgcctgc tggtcatcag cgacattttg aagaacctca atctccattg
     4381 agtgatagaa aggacgtgag ggctatgtta tgtgatgcct gtaagaaaag agaggctgtt
     4441 gtccatatcg tcaagatcga gaacgggcgg cgcacggact tgcacctgtg tgcggcctgt
     4501 gccaagaaac agactaattt ttctaatttc agagatttga atatcatcga taacgacttt
     4561 ttccgcaaga tggcttatcc cgattatcag ggacgcagcg gcgacgaacc acgctgtgcc
     4621 agctgcggca tgacctacag cgaattcaac cgcctcggga agttcggctg ccccgactgc
     4681 tatgaagcgt tcaaggaaga aatcccgccg ctcctgcgcc gcatccacgg ccacagcaag
     4741 cacgtcggca aagtgcccaa ccgcggcacc ggcgtcttcc ggacggcgac gcacgtcaag
     4801 cgcctgcgcc agcacctgca gaagctggtc caggaagaac gctatgaaga agcggccaag
     4861 gtccgcgatg agatccgcgc cctccagcgt caattgccgg acggcggaaa gggggaatga
     4921 gccatggcac atgatatgct ccagcagccg ctctgcccct ggcaggacgg gacgggcaat
     4981 ttcaatgaca tcgtcatcga cagccgtgtc cgcctggacc ggaacctgaa gaaatacgtt
     5041 ttccccgaca aagcgtcgga tacggaactg gcagcggttc tggaagaagg aaaacgccag
     5101 atcggtcccc tcgatacctt gggccatggg tgctatacct tcatcggcct cgacgaattg
     5161 tcgccgctgg aacgggaaat gctggcagtg aaacattttt cctcggccag tcatatcgaa
     5221 catcccaagc accgcggcct cctgctccgt gaagacgggg ccgtgtcgat catgatcaat
     5281 gaagaagacc atttctgcat ccagacggca gcacctggtt tcgacctcca gcgcgtctgg
     5341 gaccaggcgg cccaggtcga cgatgccctg gaaagccacc tggatttcgc cttccgcgac
     5401 gatttcggtt acctcacggc cagtccgtcc ctgaccggta cgggcctgac cgtcggcgtg
     5461 accatccacg tcccggccct gatcctcatg aagcggttca accgcatcgt ccagggcatt
     5521 actaaattcg gcttcaccgt atccgggatg tacggcgaac gcaatgaatg tatcggcaac
     5581 atcttccaga tcaccaacca gattacgctg ggcgtgaccg aaacggacat cctcgatcaa
     5641 ttgagcaaga tcgtcactca gatcgtccag gaagaacaaa attgccgcag tctcgtttgg
     5701 gaccacaatg aaaatacctt aaaagataaa tggctgcgca cgtacgggac gctcagtcag
     5761 gcctggctcc tgtcggacca ggaatcgctg gccctggcca gtgacctgcg tttcggcatc
     5821 gatatgggcg tcatcccgga acagcccctg gcctatgaag ccatcatgac cgtcgtcgaa
     5881 ccggcttatt tacagctcaa ggccggggcg gaactgaatg atgaagagct ccagcaccag
     5941 cgggcccagg ccgtccagtc cgtactcgca gcttaccgga ggaagtgaat ttcccatgat
     6001 gaacaatcgt ctcaccaatg atgccaaagc tgtcctgcag tatgcgcagg aagcggcaca
     6061 aaaattccac catgattacg tcgggaccga acatatcctg ctcggtctcg ttctcaatac
     6121 agacggcatc gccggcctgc tcctcaacca gttgggcctg acgccggaaa atgtcacccg
     6181 cgccatcgaa cagcacgtcg gctggggaca ggattcgccg acgaagctgc gcctgacgcc
     6241 gcggacgaag cgggctatgg aactggccgt ccgtgaagcc aaccggctgg aacagaacta
     6301 tgtcggcaca gaacatatgc tggccggcat cctcgaagaa gggagcggca tggccgtcca
     6361 gatcctggaa gatatggaca tcgactcgga cctggtctcc cagcgcctga gcgagcttat
     6421 gaacgatccg gccgtcgaag gcggcgatgc cgtaccgtcc aagaatggca gcgacttgtc
     6481 ccagttcggc cgcgacctta atgaatgggc caaacagggg aaaatcgacc cggtcatcgg
     6541 cagggaaaag gaaatcagcc gcgtcatcca gatcctcagc cgccggacca agaacaatcc
     6601 ggtcctcatc ggcgcccctg gcgtcggcaa gacggccatc gccgaaggtc tggcccagcg
     6661 catcatcact ggcaatgtgc ccgacagcct gctggacaag cgcattttct ccctcgattt
     6721 ggccagtatc gttgccggta ccaagtaccg tggtgaattt gaagaacgga tcaagcgcat
     6781 cctcgacacg atcgaagagg atgactcgat catcctcttc atcgacgaaa tccatcagct
     6841 catcggagcc ggtgctgtcg aaggcgccat ggatgcggcc aacatcctca aaccggccct
     6901 ggcccgcggc gatttgcagt gcatcggcgc cacgactatc gacgaatata agaaacactt
     6961 tgaaaaagat gccgccctgg cccgccgttt ccagcccgtc ctggtcggcg aaccgacgga
     7021 agacgatgcc atggctatcc tcttcggcct gcgcgaccgc tacgaagcct tccacaaggc
     7081 ccacatcacc gatgaagccg taaaggctgc ggtcaaactg tcgtcacggt acatttccga
     7141 ccgttatctg ccggacaagg ccatcgacgt catggacgaa gcggcggccc gcgtccgcat
     7201 gaaggtctat gcgccgtccc atgaacttca ggacctggaa cagaaactgg ccgacatcaa
     7261 taaagaaaaa gaagcggccc tggctggcga agactttgaa aaatgcgcgt ccctccgcga
     7321 tgccggcaag aaagtatcgt cggaaatctc ggcccttcag aaagaaaaga agcagcacga
     7381 cgatgaaaag ctcgtcgtta cggaaaacga cattgccgac gtcgtatcca tgtggacagg
     7441 cgtcccggtc caacagatta cggaaacgga atcgcagcgc ctcctccatt tggaagccga
     7501 actgcacaaa cgggtcatca gccaggacga tgccgtcacg gctgtggcca aagccgtccg
     7561 ccgggcccgc gccggcctca aggatgccaa ccggcctatc ggttccttcc tcttcctcgg
     7621 cccgtccggt gtcggcaaga ccgaactggc ccggaccctg gcagcccagc tcttcggcag
     7681 tgctgacaac atgatacgca tcgacatgag cgaattcatg gaaaaataca gcgtttcccg
     7741 cctggtcggc gcgcctccgg gctacgtcgg ctatgaagaa ggcggcgaac tgaccgatgc
     7801 cgtccgggaa aagccgtaca gcgtcatcct cttcgatgaa gtggaaaagg ccagcagcga
     7861 cttcttcaac ctgctcctgc aggtcctcga cgaaggccgc ctgaccgatt cgaaagggcg
     7921 gactgtcgac ttccgcaata cggtcatcat catgaccagc aacctcggcg catcgcatct
     7981 caagccgtcg ggcccggtca tgggcttctc gacgggcggc gacagcgcca aggaccggga
     8041 agcatctttt gcagtagcca agaaggaaat catggccgat gtgaaacgct tcttccggcc
     8101 ggaattcctc aaccgtatcg acgaaatcat cgtcttcaag ccgctggaac agaaagacct
     8161 gcgccagatc gtcgacatcc tgctcaagga cctgaccaag cgcctcggtg aaaagggtgt
     8221 cgccctcgac tggacaacgg ctgccgacga cgtcctggtc gaggaaggga cggacttcgc
     8281 ctatggcgcc cggccgttga agcgggctat ccagaagctc gtcgaagacc cgattgccga
     8341 aatgctcctc agcagcagtg tcaaggaagg caatacgatc catgtcgaca gcctggacaa
     8401 gaagaaactc gtctttacga cggagtaagg agcagtcatg gcaaagataa agacccgtta
     8461 cgtctgcagt aactgcggca gcgtgtccag ccgctggctc ggtcgctgcc cgcagtgcgg
     8521 cgaatggaat accatggttg aagagacggt cgcgccggaa gcgcccaaag cggcacagag
     8581 catggctcgc agcggtccgg cgtcgcaggc ggcgtccctg gcggatatcg ccatggaaga
     8641 catggcccgc atggaaacgg gcattggcga actggaccgc gtccttggcg gcggcatcgt
     8701 cccaggggcg ctgatcctgc tcagcggcga tccgggcatc ggcaagtcga cgctggtcct
     8761 gcagctggcc gctgccgtct gtgacaagaa cggcaccgtc ctctacggct ctggcgaaga
     8821 atcggcgggc cagatcaaga tgcgcgccca gcgcctgggc atttccacgc cggacttgat
     8881 catccaggcc gatacatccc tggaccatat cctcgacgaa gccaagaagc ggcggccggc
     8941 cctgctggtc gtcgattcca tccagaccat gtacagccag tccgtcgaag ggacgccggg
     9001 cagtctggcc cagatacgcg agaacacgag ccgcctcatg acctttgcca aaagcgaagg
     9061 catccccatc gtcgtcatcg gccacgtcac caaggacggc gccattgccg ggccgcgcat
     9121 gatggaacac atggtagacg tggtcctcta ctttgaaggt gaacggaact accagttccg
     9181 catcctgcgg ggcatcaaga accgcttcgg ctcgaccaat gaatcgggcc tgttcaccat
     9241 gaccagcagc ggcctgtcgg aactggagaa cccgtctcag ctgctcctgg ccgaacgggc
     9301 ggccgaccag gccggctcag ccgtagctgc cgtcctggat ggcatgcggc ccatgcttgg
     9361 tgaaatccag gccctgacgg cccattccgt cttcgccgtc ccgcggcgga cggcatcggg
     9421 catggactat aaccgcctga tcatcctcct ggccgtcctg gaaaagcgcc tgggcgtacc
     9481 cctgggcagc caggacgtct acatcaacgt cgtcggcggc ctgcgcctga ccgaaacggc
     9541 agccgacctg cccgttgccc tggccgtcta ttccagcctg cgggacatct ccatggacag
     9601 ccggaccgtc gtcatgggcg aagtcggcct caccggcgat atccgccgcg tcccccaggc
     9661 cctgcgccgc gtcaaagaag cggccaagat gggcttcaac gcctttatcc tgcccaaagg
     9721 caacctggaa gacatcgaaa cagacaaatt cccggactgc catttcgtag gcgtagccac
     9781 cctgcgcgaa gccatcgaaa aggccttccg aagataacct tccgctacaa actgcgaact
     9841 taatacccca aaaggcgctg tctttatgcg acagcgcctt ttcgcttgcc ttttttgagt
     9901 aaacctgtta cgctaatttt agtggctagt ggctagtggc tagtggctag tggctagtgg
     9961 ctagtggcta gtgg
//
LOCUS       DAWABZ010000059         9910 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_1856_length_9910_cov_2.904617, whole genome shotgun sequence.
ACCESSION   DAWABZ010000059 DAWABZ010000000
VERSION     DAWABZ010000059.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 9910)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9910)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9910
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1856_length_9910_cov_2.904617"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4410..4442
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9626..9654
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2066 a         2971 c         2760 g         2051 t
ORIGIN      
        1 caccattggc ggtacctggg gaatacaatc ctcttcgtca tcaacagccg cgaccgacgg
       61 gcttcgctcc aggcctggat gaagcagcat gagctgccgt tccgggacta cgatgatgac
      121 cagagtacgc caggcggcgt ctacgtcgtc gaaggggaga tccacgacgg cttcgagctg
      181 ccttatgcca aaatcgtcgt cgtcgccgaa cgggatgtct tcggcaccca gaagcggcgc
      241 ctgcgccatc atgccgccaa aggccaggaa atcaacgtct ttaccgacct caagcccggc
      301 gattacgtcg tccacgaagt ccacggcatc ggccgctaca tcggtatcaa gaccattgaa
      361 atggatggtg tccacaagga ttatctggaa atccactatg ccggcaagga tatcctctac
      421 gtgccgacgg accagttgtc cctgttgcag cgctacatcg gtaacgaagg ggatacgccg
      481 aaattacaga aaatgggcgg ttccgactgg cagaagacgc gggccaaggc ccagaagtcc
      541 attaccgacc tggcagaaaa actcgtcgcc ctctatgcca agcgggaagt cgttcagggc
      601 tatgcctttc cgccggatac gccgttccag aaggaatttg aagaagcctt tccttatgaa
      661 gaaacggaag atcagttgaa agccgtccgc gatatcaagg ccagcatgga aaagccgacg
      721 cccatggact gcctggtctg cggcgacgtc ggtttcggca agactgaagt ggccatgcgg
      781 gccatcttta aagccgtcac agccggcaag caggtcgccg tcctggtgcc gacgacggtc
      841 ttgtcgcagc agcatttcca gacctttatg gaacgcttcg gtcccttcgg cgtccacgtc
      901 gacgtcctca accgcttccg ctcgtataaa gaaaagaaag atatcctggc ccggaccctg
      961 accggcgata tcgacgtcct catagggacc cacagcctgc tcaacaagaa ggtcaagttc
     1021 aaggacctga gtctcctggt cgtcgatgaa gaacagcgct tcggcgtggc ccagaaggaa
     1081 aaatggaagg cctgggcagc caatatcgac gtcctgtccc tgagcgccac gccgattccg
     1141 cggacgctgc acatgtccct ggtcaacctg cgggaaatgt gcgtcatcga aacgccgccg
     1201 acagaccgtt tcccggtcca gacctatgtg accgaatacg acgcccgcat cgtcagcgat
     1261 gccattatgc gcgaaaaacg gcggggcggc caggtcttct tcgtctacaa ccgcgttgcg
     1321 accatcgaaa agatgaaaga cgaactccag gccctcttgc cggatgtgac catcggcatc
     1381 gcccacggcc agatggccgg gagcctgctg gaatcggtca tgttcgattt ctatgaaggc
     1441 aaatacgacg tcctcctctg ttcgagcctg gtcgaaaacg gcctggacgt agccaatgcc
     1501 aataccatca tcatttacga cgccgaccat ttcggcctgt cccagctcta tcagatgcgc
     1561 ggccgcgtcg gtcggagcca tcgcatggcc tatgcctatt tcctctaccg ccgcgacaag
     1621 gtcctgtcgg aagtcgccga aaaacgcttg caggccatca aggaattcac cgaactcggc
     1681 tcgggcttca agattgccat gcgtgacctg gaaatccgcg gcgccggcaa cctcctgggt
     1741 cgggaacagc acggcaatat cgccagcgtc ggctttgcca tgtactgcca tatgctggaa
     1801 gatgccatcg ccaaggctca gaacggcaag gaagcagcgc cgccgccacc ggaaacggtc
     1861 atggaaatcc acgtcgacgc ctttatcgac gacggctata tccaaaatgg cggccagaaa
     1921 atcgaaatgt accagcgcct ggccgtctta tcgtcgaaag aagaactggc cgacctgcgc
     1981 cgggaactgg ccgaccgcta cggcaagccc agccagcccg tagagaaatt gctgcaggtc
     2041 acggaaacgc gcctcgacgc caaggcccaa ggattggtcc tcatcgctca gaagaaagac
     2101 gtcctggaat tgaagtggca gcgcgtcgcc gatatgcccg acctttccaa actggaaccg
     2161 tccctgcgcc gccgtttccg ccttgtgccc aacatgcccg tcatggtccg cgtctccctg
     2221 gctgggattt ccgacgtcct ggcctttatc cagacgctgc tggtggcctt tttatcattg
     2281 agaaagggga attcccttga gtaagaactc ctttttacgg ggcgccatgg tcctcaccgt
     2341 ggccggtatc gtcgtcaaga tcctgggcgg gctgaaccgt atcttcttgt cgcgcctctt
     2401 ggggggcgaa ggcatcggcc tgtaccagat ggcttatccc gtctatatcc tgctcctgag
     2461 cattatcggc gccgggattc ccattgccgt gtccatcatg atcgctgagg aagcggccaa
     2521 aggccattac agcggcgtcc gccgtatctt tcatgtgacc ctggtcttca tgaccgtcgt
     2581 cgccgtcgtc tgcggcctgg ccctgccttt tggcgcccat ggcctggtcg aaatgggcat
     2641 cgtccgcgat agccgggtct ttccggctct ggccgtcctg gcgccggccc tgagcctgtc
     2701 ggtcatcgcc tgctgtttcc gcggctattt ccagggcctg cagctcatga cgccgacggc
     2761 actgtcccag atggcagacc agtttgtccg ggtctgcacc atgctcgtcc tggcctggat
     2821 tctcttgccg aaggggctgg aatgggccgc tgccggtgcc gccttcggcg ccgtcccggg
     2881 ggcggcggca gggctggtgc tcatcgcttt cctgtattac cgtcacagcc gccatcccat
     2941 gcccgatgac gacggcgtct tcgtcatcca gtcgccgcgg cgcatcatcc gccggctcat
     3001 cgtcctggcc gtgcccgtag cggcggccaa catgctcctg ccggccgtgg ccagcatcga
     3061 cttgttcatc gtgcccctgc gtctggaagc agctggctac agcacccatc aggcgacggc
     3121 cctttacggc tatctgacgg gcatggccaa cggcctggtc cagctgccgg ctatcttgac
     3181 catcgccttg gcgacgagcc tcgtcccggc cgtatcggct gccgtttccc agcggaatcg
     3241 ggacgtcatc ctcgaccgga ccgacacggc catgcgcatt gccaacgtca tcaccgtgcc
     3301 ggcctgcctg ggcctggccg tcctggccgt acccatttcc cggctcctct atgcgacgcc
     3361 ggcagcgggg ccggccatct gcgtcctgtc catttcggtc ttcctcgtcg gtctccagca
     3421 gatcaccagc ggccttttgc agggcatggg ccatacggcc gtcccgctgt acaacatggc
     3481 cgccgcggcc gtcgtcaaga ttatcctcag ctggcacttg acggccattc cctggctggg
     3541 cgaagtcggc gccgcctggg cgaccaatgc cgatttggcc gtcgctacgg cgctcaatct
     3601 ttactttgcc cgtaaataca ttgcctaccg cgtccagtgg ggctacatcg ggaaactctt
     3661 tctggccggc gccgccatgg ccggcacggc ctggctcgtc caccacgcct tgttcgtcct
     3721 cttcggcaac gccgtggcca ccatcgccgc catcatggcc gcagccctgg tctacctcat
     3781 cggcctcgtc gtcttccggg ccctcacggc agaagatatc gaaaaaatcc ccgtcgtcgg
     3841 gaagaaagta agccggcgga tacatttgta ggaaagacgc aggccgcagt ccgcgggtgg
     3901 caggccagaa aaaaatacac ggcctgcggc cgtatgcgta ggggctgtcg cacgaaggct
     3961 tcggccatca tgcgaggccc cttttacttt atagtttgta gtacggagtt tgtagaagct
     4021 ggtcttaaat tttttaccat ttgctacaaa ctaaaaacta caaacttaaa actcaaaaag
     4081 aggcgatgtc gtcagtttga atctgttgcc gcgcatgtca tcggggatgt acacaggatt
     4141 cacgcgggac gccttaacag gacgtccccc tacgatgcgg tctgcgaccg gtagggacgt
     4201 tagccccaaa actcgatgaa cggctctagg ctgcggcctg cgggacggcc tttggccatc
     4261 ccttgctttt tcccgttctt tttgctatac tggaaacaaa tagttgagca aatcaactaa
     4321 ttactgaaga aaaggggatg cagcacatgg attatttgaa ggctggtcat gatttcagtg
     4381 tcatcaacca gcgttcccaa gcctatgtgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nntcgaagcg tgccgtcctt ggaatttgtc ttattcggaa catgtgacgc tcctgagttt
     4501 gtacagccac gatggctgcc ggcagaatga gctctgtcag gtcatgcagg ccgacaaagc
     4561 cctggtggcc cggaatctga agacgctgga ggcgaaggga ttcgtcagcc ggcgccagca
     4621 ggggacggac cggcgttaca agtatctcta tttgacgccc aaggcccggg agctgcagga
     4681 tacgatggaa gagattttaa ggaaatgggt cgacgtcctc gtccagggcg tcgatgaggt
     4741 gacgctgggg ccggcgttgc agctcatgca tcaagtggcc gacaatgccg ctgaagccga
     4801 tattgctgcc cttaatccca caaaggagat gaagccatga aacgcgattg gaaatataca
     4861 gtcctcgtca tagccgtcct gacggccttg ggcctgacag gctgcggcca gcagacccag
     4921 caggccgaac agccccagct ggtcaaggcc atccaggtcg gcagtgaaac ggcgggcacg
     4981 acggccggta cctattcggg gacggtcaag ggccgatacc agagtaattt gtccttccag
     5041 gccggcggcc gcattacggc ccgcaacgtc cagctcggct cccaggtcca tgccggagac
     5101 gtcctgatga cggtcgaccc gaaagacgtg gcccaggctg ttaatcagac ccaggcccag
     5161 gtcgacgccg ctgctgctca gctccagctg gcccagtcca atttaacccg ttatcagcaa
     5221 ttatacgata tggatgccat cagcgcgtcc gtcctggacc agtaccagac agcttacgac
     5281 caggcttcgg cccagtataa ccaggccttg gcagcccagc aggcgcagga aaaccagtta
     5341 tcctatacgc agctcacggc cgatgccgac ggcgtcatct cagccgtatc ggcagaagtc
     5401 ggccaggtcg tcgctgccgg ccagactgta gtcaccctgg tccattcagg ggacctggaa
     5461 gtccaggtca atgtaccgga aaataaattg gccgattttc cagtcggcca gaacgtgacc
     5521 gtcaccttct gggccctcca gaaccagcag gccgccggtg tcgtccgcga agtggctcct
     5581 atggccgatg cggcgtcgcg gacgtaccag gtcaatattt ccctgcccaa tccgccggac
     5641 ggcatgcagc tcggcatgac ggctacggtt gccaacggca gtgaacaggc agccgctgaa
     5701 gataccttcg tcctgccgct ggccgccatc tatcagaccg gcgacacgcc ccaggtctgg
     5761 gtcgtcggca aggataagac cttgtccctg aaagacgtga cggtccagga ttttggcgat
     5821 aatacggtca aagtcacggg cctcagccgc ggtgatgtcg tcgtcacggc cggcgtccat
     5881 atgctcagtg aaggacagaa agtccgcgta gagggggaag atgaatgaga aatttatcgg
     5941 aagtctctat tcacaaccgc gtcctcgtct ggtacttcat catcctcgtc gccattgccg
     6001 gtatcttttc ctacatgaaa ctgggccgca tggaagaccc gacgtatacg gttcggacta
     6061 tggtcgtctc cgtcgcctgg cccggtgcca cggcggaaca gatgcaggaa caggtgacgg
     6121 ataaaatcga aaagaaactg caggatacgc cgaacctgga ttacatcaag agttattccc
     6181 ggcccggcca gtcggtcatc tacgtctacc tcgacggcaa ggcgccgcgc gacagcatcc
     6241 gcagcacctg gcatgaagtc cgcaatttga cggaagatat gaagaaagat ttgcccgaag
     6301 gcgtctatgg gccttattac aacgaccgtt tcgacgacgt ctacggttct atctatgcta
     6361 tcaccggcga tggctattcg tatgaagaat tgcgtcagaa agcggaaaag atacgccgca
     6421 tgatgatgtc cgtcgacgat gtcagcaaag tcgaactcgt cggcgaacag tcggaaaagg
     6481 tctacatcga agtggccaat tccaaactgg ccgaactggg gattgcgccg acgaccatcg
     6541 ccaatgccgt caaaggccag aaccagatga cgccggccgg catggtcgat acccagtcgg
     6601 acaacgtcta cctgcgcctg tccggtgtct ttgaagacgt cgaagccatc cgcaacctgc
     6661 ccatcaatgc caatggccgc gtcttccgcc tgggcgatat cgccacggtc gaacggcgct
     6721 atacggaacc ggcggacccg aagatgttct ataatggcaa gcctgccgtc ggtatcgcct
     6781 tgtccatgaa gagcggcggc aataacctcc agctcggcga aaacttgaag aagttgcagg
     6841 aaagcgtcaa atccgatttg ccggccggca tggaaatcca ccaggtatcg gaccagccgc
     6901 aggtcgtcaa agagtccatc agcgacttta ccaacaccct gcgtgaagcc atcatcatcg
     6961 tcctcgtcgt cagcttcctc agcctgggac tgcggacggg cctggtcgtc gctttctgta
     7021 tcccactggt cctgaccggc gtcttctgtg ttatggaagt agccggcatc gacttgcata
     7081 aggtttcctt aggttccctg atcatcgccc tgggcctcct cgtcgacgac gccatcattg
     7141 ccgtcgaaat gatgacggtc cagctggaga agggcatgaa ccgcgtcgat gcggcctgct
     7201 atgccttcaa ggcgacggcc aagcccatgc tgacggggac cctcattacc tgctacggct
     7261 tcatccccgt cgcctttgcc gatggggcgg cgtcggaatt ctgtaaggat ttgttcccag
     7321 tcatcacggc ggccctggtc ctgtcgtgga ttgtatcggt catggtggca ccgctcttcg
     7381 gctattacct gatcaagccg aaagtggcca aaggcgaaga gccggagccc ttgtatcaga
     7441 gccggttcta cgtcttcttc cgcaaggtcc tgacctggtg cctgacccac cgggtattag
     7501 tcatcggcgg gacagcggct atgttcatct tgtccgtcgg catgatgaaa ctcattcccc
     7561 aggaattttt cccgccctcc ctgcggccgg aactcatcgt cgaaatgacc ctgcctgaag
     7621 gctcgtccat gcaggccacg cagaaggaag catcccgctt tgcccagtat ctggacagtc
     7681 agcaggcgaa gctcaaagat tattcgtact acgtcggcga aggggcaccg cgtttcgtcc
     7741 tgaccatcga accggtcctg ccggccaata actacgccca gttcgtcgtc gtcgccaagg
     7801 atacgcagac gagggaagaa ttgacgcagt ccatcaaaca ggaaatggcc gacaagttcc
     7861 ccaacgtccg ctgcaacgtc aagaacgtgc ccttagggcc gccgtcggat taccccatca
     7921 tgctgcgcgt atccggctac gatacggata aggtcaaagc ctatgcccag cagatcgcgg
     7981 cgcgcattgc caaggacccg aatacggaaa atgtcttcct caactgggac cagaaaaata
     8041 agatcatgca cgtcgacctg gatcagaata agctgcgggc catgggcatt tccggtgaag
     8101 acattgccca gaccttgtat acggaactgt ccggggcgac ggtcgcccaa tactatgcgg
     8161 ccgataagac catcggcatc cagctgcgcc tccaggacag cgaccgcaat gacctggcca
     8221 agatcaagag cctgcccgtc tacgtaggcc aggccggtta cgtgccgctg gaacagattg
     8281 ccactatttc ctataaaggc gaagatggcc tcatctggcg ccgcgatttg aaaccgacca
     8341 tcaccatcaa cggcggcatc aagtccggta cggccaacga tgtgacgcag aaagcctacg
     8401 attccattgc cgatatccgg gaaagcatgc cctttggcta taccgtcgaa gtcgacgggg
     8461 ccctggaaaa cagccagaag tccctgaagt acctcctggt accggtaccg gtcatggtca
     8521 tcatcatcat gaccctgctc atgttccagc tgcgcagtcc gtcgctcatg ttcctggccc
     8581 tgattacggc gcctatggga ctcatcggcg tctcctgggg gatgctcctc ttcgggaaat
     8641 ccctgggctt cgtcgccgat ttgggcatcc tggccctggg cggcatgatc atccgcaact
     8701 ccattattct cctggaccag atacagaaac atatggctgc cggcgaagac ccatggcatg
     8761 ccgtcatcga ctcggcggtc atgcgcttcc ggcccatcat gctcacggcc gcagcggcca
     8821 tcctgggcat gatacccctc atgaagagcg tcttctgggg ccccctggcc gtcgccatcg
     8881 ccagcggcct cttcgtcgcc accgtcctga ccctgctggt cctgccgacc atgtacgccg
     8941 cctggttcaa agtaaagaga acgagttagc ttagtggctc gtggttcgtg gctcgtaggg
     9001 gccgtcctga aaggcggtcc gcaagggctc tccttatagg agagctgtca gcgaagctga
     9061 ctgagaggtt gctcttcata gtttgtagta cggagtttgt agaagatagt tttaaattta
     9121 agaccatccg ctaaccacta gccactagct actaatcact caaaaaagtc ctgtcgcaca
     9181 tgcggcagga cttttttgcc atgatccgaa agcctgaggc gtcagtcttc cgggtccgta
     9241 gcaatgtccg gttcgttctt gcggttgaag ccgatgatgc ggcgcggctt ttcgatgtcg
     9301 tcgtggcggg ggacggcgac gaggagcaag ttgagctggg aaatgtgctg gaccagttcg
     9361 accgccgagc cgtcggacaa ttcgccgtag aagacgatca tgttcgggtc ccagtagccg
     9421 atgtcctgga tgctgaaggt gatgtcgcca gccgatacca ggcgcccgcc ggcctgcatg
     9481 tcgtcgggca ggcccgattc aaagttgttg atcagggcga cgatgcgctg atacatgtcg
     9541 gaagccagat agggcgcttc cgggtcgtct tcttcttccg ggacggcgcc gggctggagg
     9601 atagtcagtt ccttttctgc agctgnnnnn nnnnnnnnnn nnnnnnnnnn nnnntttcgg
     9661 gggccttttg ttttttctta tcgtttacgt ctgccataaa aatccctcct tatcattctc
     9721 attatatcat ttagacatag aatgaggcaa ttcctgctac ttttttccgg gtagcaggct
     9781 ttttttcttg gcatccctat gctgatatgg tataatggtc aagacatgct gacatcaggt
     9841 aacatatttt cacacgaaaa gaggtttttt cttcatggaa tatgtgaacg agttggacac
     9901 tatatcggcg
//
LOCUS       DAWABZ010000060         9754 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_1898_length_9754_cov_3.651510, whole genome shotgun sequence.
ACCESSION   DAWABZ010000060 DAWABZ010000000
VERSION     DAWABZ010000060.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 9754)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9754)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9754
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1898_length_9754_cov_3.651510"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2216 a         2713 c         2616 g         2209 t
ORIGIN      
        1 cagctgactg agaggttaac cgctccggcc ctttgagcca cctctcctgt taggagaggc
       61 tttcgtggat gcagatttcc gagacagcaa aaaagggctt gccgcacgac gacaaaccct
      121 tttttacata tcctaggggc gggctcgcca cgtgcgagcc cctacaaact atttttaaat
      181 tccgaaacgt gcgaagatgg tgtcgacgtg gctgagcatc ttcttgacgt cgaagcagcc
      241 ttcgatttct tcggctgtca tgtatttcgt gacgtcttcg tcttttttca ggttttccag
      301 gaagtcttcc ccttcgagcc agcgtttcat ggcattgcgc tgaacccagc ggtatgcctg
      361 ttcgcggaca gcgccttttt caacgagcgt gttgaggatg atcgggctgt agatgaggcc
      421 gccagtaagg ttgaggtcct tcatcatctt gtccggatag accaggagct tgtcgatgac
      481 gcgggtgaaa gtctggagca tgtagtccag agcgatagtg ctgtctggca ggatgacgcg
      541 ttcgacagac gaatgggaaa tgtcgcgttc gtgccagaga gcgacgtctt ccaaagcagc
      601 ctgggcgttg ccgcggatga cgcgggccaa gccgcacatg cgttcacaag tgataggatt
      661 gcgtttgtga ggcatgatgg aagagccttt ctgtttgggg ctgaagtatt cttcggcttc
      721 gcggacttcc gtgcgctgga ggtgacggat ttccgtagcc atcttgtcga tggagctggc
      781 aatgacagcc agggtcgtta catattcagc atggcggtcg cgctgcaagg tctgcgacga
      841 aatcggcgaa gcagcgatgc cgagatgttc gcagacatat ttttcaacaa agggatcaat
      901 gtcggcatag gtaccgacag caccggaaat cttgccgacg gaaatcgttt ccttggcgtg
      961 tttcatgcgg tcgatgtcgc gttcgatttc agccatccag ttgcacagct tgaggccgaa
     1021 cgtcgtcgct tcggcatgga tgccgtgggt acggccgatg cacggagtat atttgaattc
     1081 aacggcgcgg cggcgcaata cggcgtggaa gttttccagg tccttgatga ggatgtccga
     1141 agcctgtttg agcatgtagc ccagagccgt gtctttgacg tctgtcgagg tcaggccgag
     1201 gtgtacatat ttaccggctt cgccgatatt ttccgaaagc gtcgagacga aagccgcaat
     1261 gtcatggtgt gtttccgctt cgatttcatg gatgcggtcg acagaaaaag cagctttttc
     1321 gcgaatttcc ttagccgctt ctttcgggat gttgcccagt tcggcctgtg cttcactagc
     1381 caggatttcg acctgtttca tcgtatccca ttcattgtat tctgtccaaa tatgacccat
     1441 ttccggtttc gtatagcgtt caatcacagt tgatctctcc tttgtagttg acatgagtac
     1501 ggtaaaaaaa ctcaccaact ttattataca ctatctatgg ggctgaaggt agccgttttt
     1561 acaagtttta ttcgattacg atggaccatt tgtcccgctc ggtcacatag ccgacgacgg
     1621 ccgcacaggg aatcgagtct ttcaaggctt tttccagggc cggtgcgtcc ttttcagcga
     1681 cagcgatgag caggccgccc gaagtctggg gatcgtagag gatgtccatg aagcgcttat
     1741 cagtgccgtc cgccttgacg cgtcccgcag caaagtcgcg gttgcggtag gctccagccg
     1801 gaatcagccc catggctgcc atgtccaatg cctgtttatg gtagggaatg gcgtcactct
     1861 gaaaatgaat cgtcgttcca ctgccgtcag ccatttcaca ggcatggccg agcaggccga
     1921 agcccgtaac gtcggtacag ctgtggactt cgtagccgac catgatgtcg cgggcccctt
     1981 tattgagctg ggccatctgg tcgtagagct gctgcaggag cggcttggcc acgaaatcgc
     2041 cctgggctgc cgtcatgagg atgcccacgc ctaagggctt ggtcaggatg aggacatcgc
     2101 caggccgggc cgacgagttg cgcaacagtt tctgtggatg gacgaaaccc gtgacggaca
     2161 agccgtattt cggttccttg tcttcgatag tatggccgcc ggtgatgatg acaccgcctt
     2221 cataggcctt ctggtagccc ccttcaagga tagcccggac ggcttcgcgg cccatggaca
     2281 ggttgacgca catgacgttc atggccagtt tggcttcggc tcccatggca tagacgtcgc
     2341 tcagggcgtt ggccgccgca atttgaccga ataaataagg atcgtcgacg ataggcggga
     2401 agaaatcgac cgtttggacg acagccagat cgtcgctcac ctgatagacc gacgcgtcgt
     2461 cgctcttatc atagccaacg aggaggcggt catctttatg tgttggaagc ccgtccaaca
     2521 attgagccag tgtccctggc cctaacttag cgcctcaacc ggagcaggac gccatttgag
     2581 tcaatttcaa acgttcatcg ttcatggtag atacctcctt gatgttacag tattcagaat
     2641 aaggtcatcg tacaaagacg gccttattgt tagctttgag tttgcagttt ttagttttca
     2701 gtgatggcta aaaatttaaa accatctgct acaaactata ggctacaaac tcattatact
     2761 atatttatgt aattcttacg gcctggttta gctcgatttt agatttatcc gttatactgt
     2821 cctcgtagtc aagaataagt gattgaagac aggaggaatt catcatgacc caggaaaaat
     2881 ggcaagatac actcgtttcg attaaaaata aagtctgcac ggcccgcaat gcaaaaatcg
     2941 ctgctgcctt ctgcgtcgtc tgcgtcgtag ccacggccgg cggccactac tatctgcacc
     3001 agcagcatct ggcccgtcac gccgaacggg tcgacgccat ggcccagatg accaaggccc
     3061 aggccgacca gcacaacatc tccctcatca gtgaagacca ggcctgcgcc gctgccgccc
     3121 aggccatcgg caaggacgaa agtgaattgg atttcaaaga aatcgccctc ttcgatatga
     3181 gcagcgccaa gcacggtccg aaagacggcc gcaaggatga ccgcaaaggc gaccacaaag
     3241 aaaaagcgaa ggacagagac cgcaaaggcg atgaaagagg cgaccagcgc ggaccagaag
     3301 gcatgaaacc tggccgtcct ggtgaagaaa aggccgatgc cgctgccggt gctacggcca
     3361 aagccgctcc cgacggccag gccggcaacc aatccgcccc catgacaccg ccccagggcc
     3421 agccgccgat gactgcttct ggacaaccgg gccagccgga tatgaaaggc cagccgccgc
     3481 agggagctat gccgcaacgc gccttccacc ccatgtataa agtccgcgtc acatccggat
     3541 ccgtccgcta cgacgtcctc gtagacgcca ctaacggcac cgtcatccat acagaaattg
     3601 aagattagct tgatacaaaa gaggctgtcc atgaagacag cctcttttgt agttttaagt
     3661 ttgtagtttg tagtttttag ttttctacaa actacaaact ccatgctaca aactatagag
     3721 aaaaaggagc cgcgcatgat ggcagaaacc ttcgtgcgac atccccctac gaatcactag
     3781 ccacgagcca cgaatcactc tataatgaaa ctatcactac gaaaggatgg cctgtttatg
     3841 ctgcatatac gtgatatttc actgcgacac cgcctgctca tcactaattt taccatggtc
     3901 tttgttccca tcgctatttt gacggccctg ggattcctac tcatctcagg actccgcttt
     3961 tcatcgcctc acaatgattt gacgacgtta tggccggaaa aggggccggc cctgtccatc
     4021 cagtatacgg ttagttccct tcgcgtcaag gccgaacggc ctgggccgtt aaaggtcaag
     4081 gatatgcgcg aagactgccg ggtcctggaa gacctgggca ttgccacggc catcgtccgc
     4141 aacgaccagg tcgcctatat cacgccgggc atcgactttt atggtctggc cgacagggtc
     4201 ctgaaaaaag cccatggcca gcagacggtc atgacctggg acggcgacgg tttctttttc
     4261 cgctacacgt ccccccacag cgggacgacg gtcatcgctg ccggcaatac gccgttcatt
     4321 gctaaaagcg gcatccgcga aggcatggcc aagggcgtca tgcaaatgct tctcctgttc
     4381 atcgtcggca cggccagcgc catcatcatc gcctgcggct tcatcctgtc ccgcgtcctg
     4441 tcccgccaga tactgacgcc gctggcagcg cttcggacag cggcagctga aatcgagaaa
     4501 ggcaatctcg actacgagct ccgcctgtcg tcgcgcgatg aactcggccg gacctgtgaa
     4561 gccttcgacc acatgcgccg ccagctgcgc tcagcccgcc tggcccagga aaagtatgag
     4621 cagaaccgca aagagctcat cgccggcatc tcccatgatt tgtcgacgcc cctgaccctg
     4681 ctcaaaggct atgccagcgg catcctggtc ggcatcgcca agaccgctga aaagcgccat
     4741 cattacgtcg agctcatcta ccagaacgcc tgcaccctgg aaaaactcgt cgacaggctg
     4801 ttcctctttt ccaaactcga cttgggccaa gtatccttta tgatggaacg ggtatcgctg
     4861 cgcgattatt tcgctgattt cgccgccgaa aacacggaac gcctggccga gcggggcctc
     4921 atcctgcact attcgccgcc agccggtccg gcctggacag ctatcgacag gatgcagttc
     4981 cagcgggtca tcgacaacct attggaaaat gccctgaaat ataaagaagg ccctacagtc
     5041 accatggacc tgcacctcgc cgaaacgccg caggccgtcg tcctgaccgc agccgaccac
     5101 gggccaggtg tcccggcggc agacctgccc cgcttattcg acagttttta ccggaccgat
     5161 gccgcccgga cggatgtcca aaagggcagc ggcctgggcc tggccattgc caaacagatc
     5221 attacgaccc tgcacggcca aatccatgca gaagcaacac ccggcggcgg cctgaccgtc
     5281 gtcatcacct taccgcggat agaggaggaa tatcatgaaa cggattctga tcattgaaga
     5341 taacttagaa attgccgaac tggaacggga ttttctcgaa gccagtgcca ttgagagcgt
     5401 catcgaaacg gatgggacga aaggcctgga acgggccctg caggaagatt tcgacctcat
     5461 cctgctggac atcatgctgc ccgggaccga cggcttccac atctgccgcc gcatccgcga
     5521 ggaaaaggaa gtacccatcc tgatggtatc ggcaaagcgg gaagacatgg acaagatacg
     5581 cggcctgggc ctcggcgccg acgactacat catcaaaccc ttcagcccga cagagctcgt
     5641 agcccgcgtc aaggctcaca tcacacgcta tgaacggctc atgggcaagg cccagcagaa
     5701 agcggccgat ggcgaagtca tccgcagcgg cgacctggaa atttacgtcg ataagcaccg
     5761 ggtctacgtc aaaggaaaag aaatcgccct gaccaaccgc gagttcgagc tcctcatctt
     5821 cctggccaag catcccggcc tggtcttcag ccgggaccga ctcttcgagc gcgtctgggg
     5881 tctcgacgcc gaaggcgaca cggcgacagt catggtccac atcaaccgca tccgcgaaaa
     5941 aatagaaccg gacccggcca agcccatcta catcgagacg gtctggggtg ccggataccg
     6001 gttcaaagaa tgacgctatt tacttttctt cccgccgcag gatttcctga ccgactgtca
     6061 cgccggctgc cgaagcctgt accaggccgc gggtgatgcc ggcgccatcg ccgatggcaa
     6121 agaagttggg aatcttcgtt tccaggacat ggctcaattc cagccggttc gagtagaact
     6181 tggcttccac gccgtacaac agggtatggc gcgagttggc accaggtgct acggcatcga
     6241 gggcttccag catttccttg atatcctgca tatgacggta cggcaggacc agcgataagt
     6301 cccctggcgt cgcattgggc atggtcggcc ggaccaggcc ccgctggatg cgttccggcg
     6361 tcgaccgccg cccgtcgagg aagtctccca ggcgctggac gatgacgccg ccgccgagga
     6421 tattggccag gttggcaatg tatttcccgt atttgatcgg ttcatggaaa ggttccgtga
     6481 atttcttgga tacgaggatg gcgaaattcg tgttttcgct cttcttgtga gcataactgt
     6541 ggccgttgac cgtgagcagg ccgtcgttat tctcttcgac gacgaaacca taagggttca
     6601 tacagaacgt gcggatccgg tcgtcaaagg atttgctgaa ataaatgagc ttcgattcat
     6661 agacgacgtc ggtaatcgtt tccagaactt ctgccggcat ttccacgcgg acgccgatat
     6721 cgacggcgtt agacgtcagc ttgaggccca tcttttcgac ttcgtggacg aaccattccg
     6781 acccttcccg gccgggagca ctgaccagat agcggcaggc atagaactgg ccgccgatga
     6841 ggacgccttt gatttcccct ttttcttcga cgatgtgatc gactgccgta tcggccatga
     6901 tatcgacttt cccttccagg aaatcgcgca tattggtcag gatctgggca ttgacatcgg
     6961 tccccagatg gcggatgcgg gccgggatga agcgcaggtc agcagcagcc gctttgcgct
     7021 tgatatcgcg gatacattcc ttgttctcat cgccatagac cttgcggccg gcaccgccaa
     7081 agcggcagta gacgtcgtcg acgtaacgta tcttgtccat gaggtcggcc ttggacatat
     7141 agtcgtcgag attgccgccg tattccgtcg tcagcgtcag tttgccgtca ctgaaggcac
     7201 cggcgccgcc ccagccacag acgacattgc gcatcttatc ctttggcagg gccttcttgt
     7261 tcaggctcgt cgcaatgcgc tcccgtatat cctgtccctt ttcgaccatg aggatcttaa
     7321 atcctttatc ggccagttcc agtgccgtaa aaataccggc cggaccggcc ccgatgataa
     7381 tgacatcata tgtctttact gcatgcataa tcacacatcc tttccaggca gaccctttgc
     7441 gaacaggagc tcacacgtgg caagcgctct aaagtggctc gtggctcgtg gtcgccccta
     7501 cggcctgcga actgcggtct gcgggccgct ctgcaaagcg gcccttaccc cctcacgcta
     7561 tcatattgtg aaaagaaagt caacctatgc tataatgagg taaaacgcta tagaaaggat
     7621 gatgacttat gtctcgtcgt aaccgcccgg ctgtccctga tgactccagc cgggatttaa
     7681 aacgccagga agggattttc ctcagcactt ttgccctcat ggtcctcttt ctcgtatcct
     7741 tataccttcc ccttcccgtg gctgtcccca tcgtactggc cgtcgtcctc gtcgcctgga
     7801 ctgtcgccat gtacgtcaaa ttccatgact tctacaagat gcgcgaccgt ggccagcgga
     7861 cgtggtgtgt caccatttcc atgtacgcca gcctcatcct gaccctggcc tgtgcctggt
     7921 atttcaccaa agatgcccct ctgacggatg aatatgccct ggtcttcctc ttcggcttca
     7981 tgttcttcac gtacatggtc taccgcaccc tgagcccgac tatggtcgtc ggcaaccgcc
     8041 gcatccggta taagtaaaaa agggactgtc gcatgtgcga cggtcccttt ttaagtggct
     8101 agcagttagg ggttagcggt tagccaatga caaacccctt ttgagttgta ggggcgacca
     8161 cgtggggcgc ccgctctgga atatatacgg taggtcgttc atcgttgatt gcataggatg
     8221 aaaaatatca agctatcacg atgaactacg aacgaactac gaaattactg ctgagcacgt
     8281 tcttttttgt agaggtcagc tgtgatggca tcggagcagc atacagcaac gacgtcagca
     8341 acgtcttcag acgagcagcc acgggacaag tcgaggatag gtttatccaa gccctggacc
     8401 aaggggccaa gagccgttgc gttagcgaaa cgctgaacca tcttgtagcc gatgttagca
     8461 gcatcgaggt tcgggaagat gaggacgtta gcgtggccgg caactttgga gcccggtgct
     8521 ttgtgttcgc caacttccgg tacgagagca gcgtcagcct ggagttcgcc gtcgaagtcg
     8581 aagtcgacgt tgcgttcttt caggatttca acagcatggc gtacgttgtc gacggattcg
     8641 ccagcgccag aacctttggt ggagtaggaa aggaaagcga ctttcggatt gaggatctgg
     8701 accgtgcggc gggcgcgttc tacgcagatg caagcgatat cagcgagctg ttccgacgtc
     8761 gggttgggga taacgccgca gtcaccgagt acgaggatac cgttatcgcc atactgcgga
     8821 gtattggtca agagaatgaa agcggaagat acggtcttgt tgccaggacg ggggccgatg
     8881 acctgaaggc ctgcgcggat aacttcagca gaagtcgtag cagcaccgga tacgcagccg
     8941 tcagcttcac cgagagcaac gaggccagct gcgaagaagg ggtagttgtt gagcatgatt
     9001 tcacgagctt tttccagggt catgcctttc ttttcacgac gttttgcgaa gtaagcgcaa
     9061 agttcgtcca tacgatagta agtttcaggg tcgacgactt cgacaccggt gagatcgatc
     9121 tggaatttag cagcatcttt tttgatctgt tccagtttac ctacgagaac gattttagca
     9181 aaaccatcag ccaaaacgcg ttcagcagct ttcaatacac gacggctgtt cgtttccgga
     9241 aggacgattt ttttctgtgg tttggaaacg cgggctttca aatcttctgt aaatctcatt
     9301 tgggtaccac tccttgtaag ttcttagaaa ttagtaggaa tatgtcctta tgctaaagaa
     9361 aatatcccat cgctaccatc cattataata caaaatttat aggaatcaaa catattatta
     9421 cgcatatata aaatgagcag aagaaataga ctatgccgat tgcgcaatcc aaaggccaat
     9481 gtgatacgta ttttatatcg ttcattgcag gggacgccct ttgagcgtcc cgccgcaggc
     9541 cgcaggtcgc aggccgcgga ccgtggcggc aaatcactcg tcatgggtag aacccctcag
     9601 acgcctgcgg cgccagctcc ccttcacagg ggagccatta gccactagcc actaacaacg
     9661 caaaaaaaac ctgtcacaaa gtgacaggtc tttttttcct ggtgggcgat aacaggatcg
     9721 aactgctgac atcctgcttg taaggcaggc gctc
//
LOCUS       DAWABZ010000061         9690 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_1914_length_9690_cov_3.028127, whole genome shotgun sequence.
ACCESSION   DAWABZ010000061 DAWABZ010000000
VERSION     DAWABZ010000061.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 9690)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9690)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9690
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1914_length_9690_cov_3.028127"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8085..8094
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2080 a         2596 c         2735 g         2269 t
ORIGIN      
        1 atataagcga agatgatgta cagccaggtc atgcggtcca ggtaaatcgg caggtcgatg
       61 ccgacataga gcatggcgat caagaggacg atggcgatga cgaagcgtac tttttcactg
      121 ggattgaact tcttgatgaa caggccaaag gcaatggcga cgaagatgaa cagcatggaa
      181 atggaagcgg cagccgcatt gggcaggacc atggatccat ccttggcaaa gccattgaac
      241 gtcgtagcga cgatatcgcc gaaagcggcg atgaccagca ggctgaagac ccagcagaag
      301 agcaggaaca ggcggcggcc cgtcgtgccg atgtactttt cgatgaggac gcccatggat
      361 ttgccgtcat tcttcatgga tgcgtagagg gctgcgaagt cctggacggc accgaagaag
      421 acgccgccga tcatgagcca taagaaagcc ggcagccagc cgtaggcagc ggcgatgatc
      481 gggcccgtga cggggccggc accggtgatg gacgagaact gattggcaaa gaccgtgaac
      541 ttagaagacg gcgtataatc ctggccatct tccaggcgat gggccggcgt cagggcgttg
      601 gggtcgatgc cccacgtctt agccagccac ttgccataga accgataggc taccgccagg
      661 atgacgatag cacaagtgac taaaacaaca ccattcatag aaattcctcc tatactccaa
      721 aaaaacaaat ggatctgtgt ggaggagctg aagatgttaa ggtaaataaa aatgaatgcc
      781 ataaaataca tcttcgcttc tgcgcatgtg tacggtataa cacgattaaa agggcctgtc
      841 aaatatacta ttaagtcatc gtcggcgttt tacttatatt ttttaagaat aaaaaagaga
      901 ttaaacgtta tgatttcgtt taatcccttt aacatattac atgtcaatgt gacaattatt
      961 tatattttat ggatttcagt ttttttcatc ctcagtgccc ggatacgctg gcctggctcg
     1021 tctggctggc ctgacggtgc tgggccgcac gcggacggcg aacccgcttc ttacgggcag
     1081 cggctttttt ggcattggcc tggaaattgg cgacgatagt ccgggccagc cagcccatga
     1141 gacagcctgt cagcaagatc ttgacagcca gcaagtccca cgtcgtcgat aacaataagg
     1201 ccaatacgac aaaaatagac gtaacgatcg ttttattcat gaacaaacac atctcccctg
     1261 tcgacataca taaaaatcat catactaact ttaattatac acgtttttca tggattaaac
     1321 aagggcttgt cgcacatgcg acagaccttg ttgagtatta agtttgcagt ttgtagtttt
     1381 tagtgaaggc tttaaattta aaaccatcct ctacaaactc tgcactacaa actatacagc
     1441 aggagggaca aatttaaaat ttgtatttat attgcagggg ctcgtacgac ggctactcaa
     1501 aagtcagccg catctggtac catgtgacgc cgccgtggac ggaggcgctg atgccttcgt
     1561 tctggaagcc gaatttcgca tagtacggga ggagcttgtc tttgcaggtc aggaccaggc
     1621 cctgtcgtcc ctgctgcctg gcgtcggcaa tggccgcttc caggagcttc ccggcatagc
     1681 catggcagcg gcacgaaggc agggtattga cgccgaaaat catctgccag gccccctttt
     1741 cgtcatgcat ggccgcctga gcgtacatgg cgtcagtcaa atcccgttca tccgtcacca
     1801 tgccgtcgac gaaggacacg aggtccccct tttcatcgaa gagcagccag aaatgggccg
     1861 cataatgggc caggcggtcc cggaattcat cggccgtcgc cgcttctgcc ggcgggaagc
     1921 aggctttctc tacagccgcc acagcggcta aatcggccat cgtagccgtc ctgattttta
     1981 taaacaatct cctctttcct atctattttc tctccattat acaggccatc gccgcctgcc
     2041 gcaacagaca gccttcaggc cttgcccttg aatttatgga tcaggctggt ccgattggtc
     2101 acgccgaagc ggtgatagag ctgggacaag cgcttcttga cggtgaaaat gaaggtacag
     2161 cgctgatatt cctgcagata gaccttatac gacgagtcgg ccctcagctg acacgaaccc
     2221 tcaaccagcc acaagtcctc cgtcagttcc ttggcccggt acgtttcctg gctcatgaca
     2281 caggggatga gccggttggc actggcccgg aagatatcgc cgacccgctg ccgcccttcg
     2341 gcactcattt cccggccggc gccgagccag acgacatcgg gcgccagata agccaagagc
     2401 aggtccaggc tggatgaggc gaaataggaa tgtaaaatac ggcgcgccaa ggcaatcaga
     2461 ctgcgccgcg tcgatgaagt cttcataaaa aggcatcgcc tccttttcct aaatgtcatc
     2521 ttttattgta attgaaaaag tgcctttttg taaaccagta tggtacgtta cctttattgt
     2581 aaattatcat caaatttcaa aaaaaaatca cctgtatttt actacaggtg atagatataa
     2641 aatatacata gtacctggcg attccgccct ttactattcg tttcagtcat tatttaattt
     2701 tattgagctg cgtcgccaat tcttctgccg acgggtcgcc gttgaccggc gtcatgatgt
     2761 tctgcgcccc caagtagagc gtaccgccgg acgccgtggc ctgctgcttg ctgtggccgt
     2821 tctggtaggc tgcggccagg ttgcccagga cgaaatagga ccgttcggca ctgctcatgc
     2881 tggacgtggc tgcgggcttg atgaatttgt tgccgttgac gtagaccgtg ccgtctttga
     2941 cgctggcgtg tttgccgctc ataattgtcg cgccgttcct gattcgatgg atggtcagag
     3001 ggagagaaga tttcaccgac aaagttgctg gcattatcgc cctgtttttc catgacccgc
     3061 tgccagatgg cggccgtcgc accgggatta tagccggcac gggtgatata cccgaaggac
     3121 aggttgtccg cttcccattc ggcagccctg tcgatatgga ccgtttcgat ctgattgctg
     3181 accgtgttga ggatgaggtt gttcaggccc gagccgccaa tcgtatcggc caggaccatc
     3241 ttgccgattt cgacattgac cttcttctgc gtcgaattgg cgacgtggtg tttctggccg
     3301 tggcccattt catggcctaa gacgacggcg atttcatcat cgttgctgac caggtcgaac
     3361 atgcccttat tgacgaccat gacgtggccc atggtgcagg ccgcattgaa cgtcgtgtcc
     3421 gggctgacat accagcggta gggcaaggtc ttgatgctgg ggtccgtctg ggcgatgctg
     3481 gcgctgaggc ggttcatgat agaagccagc cgttcatcgt aataagaatt attgaggacg
     3541 ccggtctgct tggtgacgga ctggtaaatc tcgctctgtc cttctttggt cgtgtcatag
     3601 tgcttgatct gcttgctcaa ggccgatttc acctggaagc cgccgatgac atcggtaatg
     3661 aggtcagcct ggaccggctg gaccggcacg atgcccatag gcagtgagaa cgcggccatg
     3721 gctgccacac aggcagcggc tatttttttc ttcatatgca taaaaatcac ccttcccctg
     3781 tactggaaat ctatacttcc attatacaag gaaagggtgg acttgcaagg cttaataagg
     3841 tcaggatatg tacgattctc ccccaaaaag ctaatgagga aattccaaca taacctgcgg
     3901 cgggcttgcc acgtgcaagc cccctacagg agtcacgaac cactaaaact aaatctgggc
     3961 aaagagtttc ttccgggtcc gttcggcttc ggctttgatc ttttcgatgt ccagtttggt
     4021 gaattcttta tggcgcaaga ggacttcgcc gttgaccagg accgtatcga catccatgct
     4081 gttggcactg taggccatga ggctcagggt gtcgttcttg gggtaccagt gcaggccttc
     4141 gcggtcgacg agagtgatgt cggctttata gcctgtttcc aatttgccga cgtctttgta
     4201 gcccaggcac ttggcgccgc cttcggtcag catgttgatg gccgtttcgg ccgggatgac
     4261 cagcgggtcc agggtattgg ccttgtggat gagcgtagtc aggcgcattt cttcgacgat
     4321 gtccaggttg ttgttgctcg atgccccatc ggtaccgagt ccgacagtga tgcctttggc
     4381 cagcatcttg gggaccggcg cgatgccgct ggccagcttg aggttgctgc ccggattgtg
     4441 ggcgacgcgg acgtgctttg ccgccatgat atccatatcg tcatcatcga cccagacgca
     4501 gtgggcagcc aggcagccgc agtccaatac gccgatttca tccatcaggg cgatgggcga
     4561 cttgccgtat tgctgcttga tgttttccac ttccccctta gtttcgctga ggtggatatg
     4621 gatttcggct cccagttccc gtgcctggtc gacgaattta tggagataat caggcgggca
     4681 cgtatacggc gcatgcggcc ccatcatgac cgtgatgcgg ccatcggccg tgccgtgcca
     4741 gtcgaggaag agctggcggt tttcatcgag gccggcctgg gcattcggcc cgataccgat
     4801 actgccgcgg cacaagcagg cgcggatacc cgattcttcg acggcgcggg cttcctggtc
     4861 catgtggtcg tacatatcgg cataggccgt cgtaccgcat cggatcattt cggcaatgcc
     4921 cagcatggac tgggcgtaaa tgacatcgtc tgtcaatttg gcttcagccg gccagatttt
     4981 cttttccagc cagtccatga gctgcatgtc gtcggcataa ctgcggaaaa ccgtcatggc
     5041 tacatggtta tgggtgttga cgaaacccgg cagggccagc atgcccttgc cgtcgatgac
     5101 ttcatcatag tccgatgcct tcggcgcttc gggaaatccc gtgatgcgcg agccgtcgat
     5161 ggcgatgttc tggccttcta agacgccgtg gtcctggtag agggctacgt gctgaatcag
     5221 ttttttcatc gtttcccctc ctggtcctta gtcgagattc aaatatttac gctgttcttc
     5281 gctgagcgtg tcgatggcat aacccatgga agccagtttg atcttggcga tttccgtgtc
     5341 gatttcatgg ggcaggacat agacatgatt gcccagttcg gcatggtgtt cgaggatgta
     5401 tttagcagcc agggcctgga cggcaaagga caggtccatg atttcagccg gatgaccgtc
     5461 accggcagcc aggttgacca ggcggccttc agccaggagg ttgatgacat ggccgttcgg
     5521 gagttcatag ccttcgatgt tgtggcgggc ttcaaagtgg cggactgcca gggcttccaa
     5581 ttcgggtttg ttgatttcta cgtcgaagtg accggcattg gccaggacga cgccgtcttt
     5641 catgttcttc atgtgttcgc cacggatgac gtccttgtcg ccagtcaggg tgacgaagat
     5701 atcgccgact ttagcggctt cagccatggg catgacttca aaaccgtcga aaacggcttc
     5761 gatggctttg atcgggtcga cttcggtgac gatgacatga gcccaggcct ttggcacgca
     5821 tagcgcagcc cttgccgcac cagccatagc cggcgacgac gactttcttg ccgacgacgg
     5881 tcaggttcgt cgtgcgcatg atgccgtccc acgtggactg acctgtgccg tagcggttat
     5941 caaagaggta tttgcagtac gaatcattgg ctgcgaccat ggggaacttc aattcgtcgg
     6001 ctttgtcgag gccgtggaga cggttgacgc ctgtcgtcgt ttcttccgtg ccgcccagga
     6061 gattttccat ggcatcggcc cgcgtcgtgt gcaggagatg gacctggtcg ccgccgtcgt
     6121 cgatgatgat gtccggatga tggtccaggg atttattgag gaaatcgaag tattcttcgt
     6181 ccgttgcgcc ataccaggca tagacggtca agccatcttc gacgagggct gcagcgacgt
     6241 cgtcctgcgt ggacaagggg ttgctgccgg tgacgatgac gttggcgccg gcgcgatgaa
     6301 ggacttctgc caaataggcc gtcttggctt cgagatggac gctgacgcaa atcgttttgc
     6361 ctttaaaggt ctggtcggct ttcagtttgt cgccaatggc attgagggcc ggcatgtgac
     6421 gatcgaccca gctgattttt tcatgaccgc ttttagctaa cttgatatcg cgaataatag
     6481 attccatacg agtaagacct ctttctgtaa tgaattagtg ttggaaaaac gccggtacgg
     6541 atatggcatc cggccggtgg atgatgccct gttccgtcac gatggcgcgg atattttcat
     6601 gaggcgtcac atcgaaggcg ggattgaaga cgggcgtttc ggggagggct gccgccgcac
     6661 cccggaaaga acggacttca tcgtgattcc gttcttcaat agggatctgg ctgccgtcgg
     6721 caatggacaa atcgaaagtc gaagccggcg cggcgatgta caggggaata tccagttccc
     6781 ttgccatcag ggacaggccg taagtcccga tcttattggc cgtatcgcca ttggcggcga
     6841 tgcggtcggc accgacgatg atggcatcga cgcctttcgt cttgagcgtc caggccgcca
     6901 tgttgtcggt aatgagcgtc accgggatat ggtcagccat gagctcgaaa gccgtcaggc
     6961 gggcgccctg caggaggggc cgcgtttcat cggcgtagac catggacacc tggccccggc
     7021 gatggagctc gcggatgaca cccagcgccg tgccgacacc agccgtagcc agggcaccgg
     7081 cattgcagtg cgtgagcagc gtcaaaggac ggctgatttt ttccagctcg tcggccccga
     7141 accgggccat agccgtattg atggcaatat cggctttatg gatggccacg gccttttctt
     7201 ccagggctgc cgtcgtggct tcagcgccgt cggcttcatg ctggcgggca caagccatca
     7261 tctgggtcag ggcccagtgc aggttgaccg ccgtcggccg ggccgcatcg aggtcccggc
     7321 agatagtttc caggtccgtc aaaaagggcg tatctccccc tttctggcac agggtacgcg
     7381 ccgccaggac gacggcatag gccgcagcga cgccgatagc cggcgccccg cggacagcca
     7441 ggacggaaat ggcctggcgc acgtcctgcc aggaatggca gggcagatag atttcttcaa
     7501 agggcagctt cgtctggtcc aggaggaaca gcgtgcccgc ttgccagcgc agtgtatcca
     7561 ttataccgta aaccctccat aagcctgcag ggtgtggcgg caggcgcagt cctcattatc
     7621 atataattcg atgctcttca ggatgagctg ctgcaggcgc tgggaattct cggccatagc
     7681 ttcgacgacc tcttcatggg tcaggggcgt cggcgaaatg ccggcggcaa aattggtgac
     7741 catggaaatc gtggcatagc agatttccgc ttctctggcc aggcagactt cggggacgtt
     7801 ggtcatgccg accaaatcgc cgccgagctt ttcatacatc ctgatttcag ccggcgtctc
     7861 gaagcggggc ccttccgtgc agacataggt tcccttatca tggagcgtat aaccgccaag
     7921 ggcctgagcc gcagccacta cggtccggcg cagggtcggg cagtacggtt cggtcatgtc
     7981 cacgtggacg acgccgtttt cgccaccgtc gaagaacgac gtatgacggc atttggtgaa
     8041 atccaggaac tggtcgggca ggacgaaatc gcccggcttg tcatnnnnnn nnnncgacag
     8101 ccgtcgtcga caagatggcc gtgacgccca ggcgcttcaa gcccaggata ttggcccggt
     8161 aattgatgag gtgcggcgga atggaatgat tccggccgtg gcggggcaaa aagacgagtt
     8221 ccttatcctt atacgtcccc tgccaataat gaatggtccc gtaaaaggta tccatatagt
     8281 cttcatggac attggacaaa atcttcgggt cgcagacacc ggtaccgccg atgatggcaa
     8341 tgagtcccat cgtaaaacct ccttcggaga atcaattctt cttatggcgc tgggcagccg
     8401 cttcatacag cgctgccagt tcatcgtgag acaatacgta tttggccccg caatactgac
     8461 aggtcatttc cacctgcggg tccttcaaga gggcttcctt atcttcctgg cgcagggaca
     8521 acagagcatt ttcaaagcgc tggcgcgagc acgtgcagcg ccagtacaga ttctgccggt
     8581 agagttcctt gaaatgcata ccgctgagga tgatgtcaat gaggccttca ccattgggat
     8641 ggtcctgcaa atacgtcgtc aaagcaccga cctgggtgat attggcttcg acacgggcca
     8701 ggtcttcatc cttcgcgccc ggcagggcct ggacgaggaa accgccggct gcagcaatgg
     8761 tgtaatcctg gctgaccagg acgcccaggc tgatcgtcga cggcacttgt tccgatgtga
     8821 acagatagta agcgatgtct tcggcaattt caccggacac caaatcggcc tggctcgtat
     8881 aagtcgtctt ttccggcgtg aaacgcgtca ccgtcaactg gccgtgctgg ccgacaccgg
     8941 ctgccacatc gagcttgcca ttgaccttca gcggcaggtc gacctgcgga ttgtctacat
     9001 agccgcggag cttattcgta tcgaagcagt ctgcatggac ggcgcctaag ggcccgtcac
     9061 cagcgacctt cagggaaacg ccttcgtggt tcttgtaatc gccagccaac agggctgcgc
     9121 cggtcatcgt ccggcccaga gccgcactag ccacaggcga caaattatgg cgctgccgtg
     9181 ccttctcgac gatacccgtc gtcaaggcaa tagatacccg gatgagctca tccgatgtat
     9241 aatgaatgat ctggttgcgt gtaatcatgt atgttctatc ctttctttac aaatccagtc
     9301 tctattatac aacatcggtg cagacctagc cagtgccttt tgcatgtgtt ggggagccta
     9361 tcggccccgc cggtcgcagg ccgcctagag tgaccctcgt ctcgtgaaaa aatagtggct
     9421 cgtggttcgt ggctcgtggc tcgtaaaagc ctgccattat actcgaatca gctgtggtcg
     9481 aatctctctg gcgggcttcc tacaggaagc ccctacaata cggccaccca ggccgttttt
     9541 tttgcgaact gcgaccggcg aactgcgttc atttcatgaa tctgtcgcgg cggaacgccg
     9601 catctgaatc caaaaaagac cacacatgat agccgctgat gtgaccccaa aaagttagac
     9661 cttggtaacg agacagtttt tactgtctcg
//
LOCUS       DAWABZ010000062         9641 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_1934_length_9641_cov_2.794492, whole genome shotgun sequence.
ACCESSION   DAWABZ010000062 DAWABZ010000000
VERSION     DAWABZ010000062.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 9641)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9641)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9641
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1934_length_9641_cov_2.794492"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1833 a         2675 c         2923 g         2210 t
ORIGIN      
        1 tagccaaagt ttatgatgat gccagaatgg gctttgatga aatctcgcaa cgggccaaaa
       61 aggcttggga cagcccctac gcaaccccaa tcggggttag ttgattgttt tgtttctgtt
      121 tatctttcgt ctcagctata attttttgca tacagaaaca ggacctgtgc acagttatgt
      181 gcgcaggtcc tgtttaggta atcccgattc aattagtgag ctctggtcaa caaggccacg
      241 gactcgacgt gatgggtccg cgggaacatg tccacgggct gtactttggt ggtgtggtag
      301 ccgtgctggt cgaggtaggc caggtcgcgg gccagggtgg ccgggttgca ggagacgtag
      361 acgacgcgtt tgggtttgct ctgggcgatg gctttgagga ccggttcgcc gcagccggcc
      421 cggggcgggt cgaggacgat gacgtcggga cggacgccgt ttttgataag ttccggcagt
      481 ttgtagatgg cgtcgcctaa gatgaactgg gcgttgccga cgcggttgtc tttggcgttg
      541 cggacggcgt cgcggatggc cggggcgacg atttcgatgc cgtagacctg cttggctttc
      601 tgggccagga acagggtgat ggtcccggtg ccgcagtaga cgtcggccac ggtttcgccg
      661 cctttgaggt cggcaaagtc gagggccgtt tcatagaggc gctgggcctg ttcgctgttg
      721 acctggaaga aggactgggc ggaaatgtgg aaggtcaggg gtccgatgga gtcgtggatg
      781 gtccctttgc cgtagacggt cttggtcttg ggtcccaaga tgacgttggt gtggcgcttg
      841 ttgacgttct ggacgacgct ggtcaggccc ggtatgtccc ggcgcaggcg ctggaccagg
      901 tctttcatgt gcggcaccat gtcgcaggcc gtgaccaggc agaccatgat ttcacccgta
      961 tggacgccga cgcggcccat gatgtggcgg acgatgcccg tccgggcgtc ttcgtcatag
     1021 gcggggatct tgaagtcctt catccactgg cggacgacgg cgacgatggc gttgttccct
     1081 tctttctgga tagcgcagtc cgtcacgtcg atgacttcgt gcgtagccgc agcgaaacag
     1141 ccgatagcga ctttgccctt gccggcagcg gcgacgggga actgcatctt attgcggtaa
     1201 tgccagggcg tatgggcgcc cagggtcggc aggacgtcga ggccctgcag gtggccgatg
     1261 cgttccagcg catcccggac ctgctggcgc ttttccttca attccccttc atagctcagg
     1321 tgctggagct ggcagccgcc gcagcggtcg tagacgggac actggggctg gatccggtcc
     1381 ggcgagggct tcaggacttc gaccagggaa gccgtggtat aggtcttctt gaccagggta
     1441 atgcgggccc gtacggtttc accgggcagg gcgaagggca cgaagaccgt aaagccttca
     1501 tagcgtccga cgccttcacc gctgctgccc aggcggacga tggatatatc ataggttttc
     1561 cccttctgga cggggatttg ccgtgtttct ttcatgggat tcctttctat ttagacgatg
     1621 atttcgctga tgaccggttc ctggacggga gcctgttcgg ctgtgacgat gatgtggtcg
     1681 gcgacctgcc gggccaggtc catcatatcg gcgccttcct tgccgtacaa ggtgaacagg
     1741 gcttcgccgg gacggaccgg gtcatagagc tctttgaaca ggcggatgcc gaccatgggg
     1801 tcgatggcat cttccttgcg ggcccggccg gcgcccatag ccatggcgat ttcgccgagg
     1861 gcccggccgt cgatgcgggt gatgtagcct tcatcggtgc agacggccgt aaagacctgg
     1921 ggcgccttgg tgaggggccg cttgccgatc cacgacgtac tgccgccctg ggccttgacg
     1981 aattcccaga acttagccag gcccttgccg gaagcgacga ggtcgcggat catggctctc
     2041 ccctgccttt cgtcctgggc cttgccgccg acgatgagca tctgggcgcc gatggcttcg
     2101 acgacttcca tgagccgccg gccgcctttg cccgacaggg cttcgacggc ttcatcgact
     2161 tccaggcagt tgccgatagc cgtgcccaga ggcgcttcca tggacgtgac gacggcccgt
     2221 gtcggccggc cggccaggtt gccgatgccg accatggtcc gggccagttc ccgcgcccgt
     2281 tcttgggtct tcatgaaggc gccgtcgccg tatttgacgt cgaggacgat ggcatcggca
     2341 ccagaggcga ttttcttgct cataatcgag ctggcgatga gagggatgct ctctaccgtc
     2401 gccgtcgcgt cgcgcagggc gtagagcagc ttatcagccg gtgcgatttc gccggactgg
     2461 gtgataaggg acaggccgat gcggcgcact tgttccagga actgttcttc cggcaggacg
     2521 acgtggaagc cggggatgga ttccagttta tctgccgtgc cgcctgtgaa gcccaggccg
     2581 cggccgctca tcttggcgac ggggacgccg gctgccgcca ctaagggcgc gacgatgagc
     2641 gtcgtcgtat cggcaatgcc gccggtgctg tgtttgtcga ctttgatgcc cggaatggac
     2701 gacaagtcga ccatatcgcc ggaatgggcc atcgccaggg tcagttcctt cgtttcttca
     2761 taagtcatgc cctggaaata gacggccatg agccaggccg ccgcctggta gtcggcgacg
     2821 cgccctgccg tatagtcagt gatgaagcgc gagatttctt ctttcgtcag gaccttgccg
     2881 tcccgtttgt gttcgatgca gtcgatgggc cacataggat cacctcagtt ccgataaaaa
     2941 gctggtaccg aattcaaggc ctgtcaggcc gaaattatcc atgatgctca tgcccaggtc
     3001 ggcataggtc tggcggagcc ctaagtcgac ggattccttc agggccgggc tgtagagcag
     3061 gagcggtacc cgttcgcgcg tatggtccgt gccgtgccag gtcgggtcgt tgccgtggtc
     3121 cgacgtgatg accagcagtt cgtcgtcgtg gatttccgtc aggagctggc ccagttcctg
     3181 gtcgaaggct tccaggcagt cggcatagcc ctggacgttg cggcggtggc cgtacatgct
     3241 gtcgaagtcg acgaagttgg ccatgagcag gcccttatcc tggcgatgag ctgccagctg
     3301 ggtccgcagg gcttccatgt cttcgtggtt gttggccgtg tggtacgatt cggtcaggcc
     3361 gatgtgggcg tagatgtcgc cgatcttacc gacgccgacg acggcataac ccgcttcgga
     3421 caaataggag aagaccattt tccgcttggg cagacggctg taatcgcggc ggtcaccagt
     3481 gcggacgaaa tggccctggg tcccgatgaa cggccgggcg atgatgcgcc ctacttcata
     3541 agggccgaca cagacttcgc ggcgcgtaat gtcgcagatg cggtacaatt cttccagggg
     3601 gatgacgtcg ttgtgggcgg caatctggaa gacgctgtct gccgacgtgt agacgatggg
     3661 caggcccgtg cggaaatgtt ccggccccag gcgttcgatg atttccgtgc ccgacgcgat
     3721 ttcattgcca atgtagccgt agcccgtctt ttccgtaaag gctttcaaca ggtccggcgg
     3781 gaaggcatcg gggaaggtcg ggaagggatt ttccacggga ttgcccatga attcccaatg
     3841 gccgctggtc gtatccttgc cggccgagat ttcctgcatg aggccatacg agccgacgac
     3901 atcggccggt tccgcatgga tatgggcgac gcggcccaga cccaggcggc gcagattagg
     3961 caccttcaag ggtacattgg cgtcgatgtg accatacgtg tcggctcctt tgtcgccgaa
     4021 tttttcggca tccggtgcat cgccggcgcc gacgctgtcc gtgacgatga cgatgatgcg
     4081 attgaatttc atagggttcc ctccttataa tcccggcaga tatttgcggg ctttttcaaa
     4141 agctgcgata gtcttggcat cggtgatttc gttcttatcg atgaggtctt ccatttcctt
     4201 gggcgtctag tattcgacgt tcatgaattc gtcgtcgtcg aggtgctgct tccctttctg
     4261 gaggccccgg gccagataga ggtaaatccg ttcactgcag aatcccggcg acgtataggt
     4321 ataacctagg ggcaggattt ccgaagccgt gacgcctgct tcttcttcca gttcccgggc
     4381 ggcgcagtaa tcgggcgatt cgccgtcgtg ttcgattttg ccggccggca cttccagcat
     4441 gacctgggcg gcagcatagc gatactggcg gacgaggata atcttgccgt cattggtcag
     4501 cggcaggatg gcgcaggcgc cgaaatgcca gaccgcttcg cgcgtggccg tcttcttttc
     4561 gccgccctgg ccgtcgtcga tggaaaccgt atcgaccgtg acgtgcagga cccggccctt
     4621 catgatttct ttacgagaaa cgagtgtttc tttgctgata ctcacttgat tcatcatcct
     4681 ttcaaaaaaa ggctccccta ataggggagc tggcagccgc aggctggctg agaagttgtt
     4741 ttttcagtgc aacccttccg gcccgttggg ccacctcccc tatcagggga ggcaaaaccc
     4801 tccagatagg tatattatct cattttcact tttttacttt cccgtgctgc cgatgccgcc
     4861 agtgcggata ccactggcag cgtcgtcgtt gacggtcagg tacttcatga agataccctg
     4921 accgatgcgt tcgccctttt ccagggtata ggcctgatca ttggtattgt aataggcaat
     4981 catgatatgc ccttcattgt cggcattgtt gtagtaatcg ctgtcgacga tgcccgtgct
     5041 gttggccagc atgaggccct tcttgaaggc caagctggag cggatgaaga tggaaaggta
     5101 ttcatcgtct tccatgaagg ccttcagccc agtcgggacg acggtgacgg catgcggtgc
     5161 caggaccgtc gtttctgcgg cttccaagtc atagccggca ctcttggccg tcttgcggac
     5221 gggaagatgg atgccacagc cttcataggc cgtgacgatt tcaaatcctc tgcctgccat
     5281 aagccctacc gtttctgacg gtcgtggttg tgaccgccgc ggggaccccg gctgcgatgg
     5341 gacgggcctt cgtcgccgcc gcccaggagg gctttgcggg acaggttgac acggccctgg
     5401 ctgtcgattt cgacgcattt gaccatgatt ccatcgccga cgtggacgac gtcttcgact
     5461 ttttcaaccc gttctttagc cagctgggaa atatggacca ggccttcttt gcccggcagg
     5521 acttcgacga aagcgccgaa gttcatgatg cgcgtgactt tgccgacgta ggtcttgccg
     5581 acttcgacgt cttcggtcag tttttcgatc cattctttgg ccttctggcc gctttcgccg
     5641 tcaacggcag cgatgtatac caggcccgaa ttgtcgatgt cgatcttggc gcctgtttct
     5701 tcggtgatct gcttgatcat cttgccgccc ggcccgatga cgtcgcggat cttatctggc
     5761 ttgatgtgca tggagatgat gcgcggagcg tagggagaga gctcctttcg cggttcactg
     5821 atgacctgca tcatcttgcc catgatgtgc atacggcctt cgtgggcctg tttcagagcc
     5881 tgggccagga tttcacggct gaggcctttg atcttgatat ccatctggat cgccgtgacc
     5941 cctttcatgg taccggcgac tttgaagtcc atgtcgccca aggcgtcttc catgccctgg
     6001 atatcggtca ggatagtgaa gtcatcgcct ttggtgacca ggcccatagc gacaccggca
     6061 acgggcgctt tgatgggcac gccggcatcc atcagggcca gggtgctgcc gcaaacgctg
     6121 cccatggagc tggagccgtt cgattcgagg acttccgata cgacgcggat ggcgtacggg
     6181 aattcttctt cactgggcat gacctgggtc aatgcgcgtt ctgccagttt accgtggccg
     6241 atttcacgac ggccgggacc gcgggacgat ttcgtttcgc cgacgcagaa ggacgggaaa
     6301 ttgtagtggt gcatgtagcg gcggtctgtt tccaggccga cgccgtcgat gtgctggcat
     6361 tccgacagcg gagccagggt gcagaagctc atgatctgcg tctggccgcg ggtgaacagg
     6421 cctgtgccgt gagtgcgggc aaagaggccg acttcgcagc tgaccggacg gacttcgttg
     6481 acacggcggc cgtcaggacg gatcttgtcg acggcgatca tgtggcggac gatttccttg
     6541 atcatcttct gcgtgacttc agcgacatca tcggcgttgt ccggatagat gtcggcaaag
     6601 tgttcataaa tgtcggcttc gacagcgtcc atcttttctt cgcgttcgac tttgtcagcc
     6661 gtgaagatag ccgtcttcat ggcttcttcg ccataagcgt ggacagcttc gacgatatct
     6721 gccggcggtt ctttttcgac gaattcctgt ttcggtacgg aaatcttgtc gaggaagctg
     6781 agctggaatt cgacgacttt cttgatttct tcatgggcga acatgatggc gtccaggacc
     6841 tgttcttccg ggacttcgtg agcgccgcct tcaaccatga ggatggcgtc ttttgtaccg
     6901 gcaacgacga tatccatgtc gctctgttca ctctgggcga cggtcgggtt gatgatgaac
     6961 tggccatcga cgcggccgac gcggacaccg gcaatcgggc cggcaaaggg gatatgggac
     7021 atgcacagcg atacggatgc gccgatcata gcggcgattt ccggagcgtt gtcatggtct
     7081 acgcagaaat cataagccac gacgtggacg tcatgacggt atgtcttcgg gaagagcggg
     7141 cggatcggac ggtcgatgag gcgggcaaaa agggttgccg attcaggcgg tttgccttcg
     7201 cgtttgatga agccgccggg aatcttgccg acggagtaca tcttttcttc gtaatcgaca
     7261 gtgagcggga aaaagtcagc gtcttcacgc gcttccttcg agcccgtagc cgtgacgaaa
     7321 acggccgtgt cgccataacg gaccaatgcg gcaccgctgg cctgtttagc cagtttgccg
     7381 acttcaatca ccagcgggcg gccggcgaaa tccatttgga atttcggttc ttccattgtt
     7441 acctccttga attcacaggt gataggcact agtccgggaa catcggcgat atacccaggc
     7501 tacaacctac caccctgtgt cctgaaaaaa gaggctgatg cagtcacatc agcatcagcc
     7561 tcacagtatg cgagattatt tacgcaagtt caatttttcg agaattgcac gataacgatt
     7621 gagatcgtgt ttctgcaagt aaccgagcaa gttacggcgc tggccgacga gtttcaacag
     7681 gccgcggcgg gatgcatggt cctttttatg gctgcggaga tgttccgtca aatagatgat
     7741 gcggctcgtg aggagcgcaa tctgtacttc cggagaaccc gtatcgtttt cattggcacc
     7801 aaatttagct acgattgctt ttttttcttc tacgcttaac attgtatctt tcctccatgt
     7861 gtaaaaaaat tgaatctatt cgcaaagtgc taagtgatcg tcggagtcat cgagacaccg
     7921 agcacccgta cgacatagct attatactat acccgggcgg ttgtgtaaag acccgggcgg
     7981 ttgtgtaaag gggattttcg tggttcgtgg ccagcggcta gcagccagct gctcatatgg
     8041 ccttgtcaaa aaggccgctt tcgcttatac tatactaaaa aggttgtatt taatattttg
     8101 ttagaaataa aggagcgcta tttatgaaga aatatgatgt cattgtcatc ggtacggggg
     8161 cagccaatat tgtgaccgat gcggccctgg cccagggctt gcaggtggct gtcattgaac
     8221 gcggccggtt cggcgggact tgtctgaacc gcggctgtat cccgacgaag gtcatggtca
     8281 cgacagccaa ccgcatccgc gaaatccgcg aagggagccg tatcggcgtc tttgccgaca
     8341 acgtccgcct ggattgggat atcttgtcca agcgactgtg ggaaaaggtc gatgaaagtc
     8401 cggctattca aagttattac gaccagttcc ccaatgtcga cacctttaac ggcacggcgt
     8461 ctttcgtcga caaccacacc gtcgccatcg ccctgaatga cggcagcagc gtcactttga
     8521 cgggcgataa gattttcgtt gctactggcg gccggaccaa tatcccctcc ctgccgggcc
     8581 tggaagaagc gggctatttg acgtcggaat cgttcttcgg cccgtcgtat ccgaagaagc
     8641 cctaccagag tctgattatc atcggtggcg gccctatcgg ctgtgagttt gcccacgtct
     8701 tcgacgccgc cggcacgaag gtcacgatgg tccagcacaa tgtccgcctc ctgcccaagg
     8761 aagacccggc cgtatcggca ttcatcctca agcagttcca gcgctacggc atggatatcc
     8821 gcctcaataa agacacggtt tccgtcagcc agcgcgacgg cttgaaggtc ctgtccttcc
     8881 gcgaccgcac gacgggcgaa gaaggcgaag tggctgccga agaaatcctg gtcgctccgg
     8941 gcatccggcc catgacggaa ctgctgcacc tggaaaatac cgacgtccgc ctggacaagc
     9001 gcggctacat cgaaacgaat gaattcctgg aaacgacggc tgaaaatatc tgggccctgg
     9061 gcgacgtcaa cggcctggcc cccttccgcc acaaggccaa ttacgaagcg gaaatcctgg
     9121 cacacaacct cttttccgga aacgaaccgg aaacgtggcg ctgggccgat tacgatgccg
     9181 tgccggccgt cacctacacc tatcccgaag cggctcacgt cggcctgacg gaagaccagg
     9241 ccgtgaagaa aggctatgac gtcgaaacgg ccatcaacca ctattcatcg tcggccaagg
     9301 gctacgccat gggctacgaa ccgggcgatg aagacgacgg cttcgtcaaa ttggtcatcg
     9361 acaagaagac gaaattcatc ctcggcgccc acatcgtcgg ccccgaagca tccatcctca
     9421 tccagccttt catcaataac ctcatgagcg gcacccacgt cgtccatccc atccacgaag
     9481 acatcgcgtc gccgacggtc caggccctgc gggccaaacc gctgacgcgg accctcaatc
     9541 ccaaatcggt ctacaccttc agcgaaacca tgacccctca cccgtccctg tcggaagtcg
     9601 tcatgtggac gcggtattat tacgaaggga agtaggaaaa a
//
LOCUS       DAWABZ010000063         9640 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_1935_length_9640_cov_3.137402, whole genome shotgun sequence.
ACCESSION   DAWABZ010000063 DAWABZ010000000
VERSION     DAWABZ010000063.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 9640)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9640)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9640
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1935_length_9640_cov_3.137402"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6152..6161
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2180 a         2616 c         2732 g         2102 t
ORIGIN      
        1 gaattcatat gaagctcaaa aaattgcact atactgtcga ggacggtatt gctgtcgttc
       61 agctcgatta tcccaagaat ctcaatgcca tcgatgaaga aatggccgat gaactgctct
      121 acgtcgtcca ggcggctgaa gccgatgaat cggtgaaagt cgtcgtcttc aaaggttctc
      181 cccgtgcttt ttcggctggt ggcgacatcg gctacttcta ccagctcatc aaggccggcg
      241 gtgacgtcaa tatggacggc ctcatcggca aggtaggcaa ggtcacggac ggcctgaaga
      301 agatgagcaa gatggtcatc gcctcggtca gcggtgccgc agccggtgcc ggtgccagcc
      361 tggcactgac ggcagatttt atcatttgca gtgaaaacgc caagttcatc atggccttcg
      421 tcaacctggg cctggtcccc gatacgggtg gttcgtattt gctggtcaaa cagctggggc
      481 cgaaacgggc catggatatc tgtgcgaccg gccggccggt gagcgctcag gaagccaagg
      541 acctgggcat cgtctaccgc atcgtgccca acgaggacct cgacggtgaa acgatgaagt
      601 ttgccaagaa attggcgtca gggccgatca tttcctacaa gaatatcaag cgccagatct
      661 atgcagccgc ctttgccgaa taccagcact acctggaaaa tatcgaagtc ccgtgccagc
      721 gcgaatgtgc ccggaccaag gatttcgccg aaggcgtctg cgcgttcatg gaaaaacggc
      781 gtccctattt taccggccag taaggctgct ttgtgaaggg agtgagtacg gtgttatcga
      841 aagtattttc tctccaagat atcctggaac atatccacga tggccggacc atcatgttcg
      901 gcgactggca cggccagttt gcagccgatg agatcatcga cggcatgctg gaaaaaggcg
      961 tcaaggatat caaagctatc gccgtatcgg cgggctatcc cggccagggc gtaggcaaac
     1021 tcatcgtggc ccatcgcgta tcgtccatca ttacgaccca tatcggcctc aacccggaag
     1081 cactgaaaca gatgctggca ggtgaactgg ccgtcgaatt cgttccccaa gggacctggg
     1141 ccgaacgggt gcgctgcggc ggtgccggat tgggcggcgt cctcacgccg acgggcgtcg
     1201 ggacgagtgt cgaagaaggg aaacagaagc tggtcatcga cgggaaagaa tacctcctgg
     1261 aattgccgct ccatgccgac gtggccctgg tcaaagcgac caaagccgat acggcaggca
     1321 atctctattt ccgcatgaat tcacgggcga cgaacagcac cattgcctat gctgctgatt
     1381 tcgtcgctgc cgaagtcgaa gaaatcgtcc ctgtcggcca gctttcgccg gaagaaatcg
     1441 ccattccggc gcccgtcgtc gatatggtct atgaacggca gggcgaaaaa cgctatgtct
     1501 gcccgatgtg gaaaaaggcc agggcccgtg ccgaagctaa agcgcgggaa cggcaggaaa
     1561 ggggatgagg tcatggtcga acggaaagga agagctttga ttgcctggcg gtgtgcccaa
     1621 ttcttcaaaa atggggattt cgtcaattta ggaatcggcc tgcccctgat gtgcgtcaac
     1681 tacctgcctg aaggcgtaga cctctggctg gaagcggaaa taggcacggt cggcagcggc
     1741 ccgtcgccgg attgggacca tgtcgacatc gacgtcatcg acgccggcgg ccagccggcc
     1801 tcagtcatta ccggcggcag cgtctatgac cacgaaacgt cctttgcctt catccgcggc
     1861 ggtcatatcg acgcgactgt cctgggaacg ctgcaagtcg accaggaagg gaatatcgcc
     1921 aactggacca ttcctgggaa attcgctccc ggtatgggcg gggccatgga cctctgtgcc
     1981 ggtgtcaaga aaatcatcgt cgccacggac cattgcgaaa agagcggcca ttccaagata
     2041 ctgaagaaat gtacgctgcc cctgacggga gcccattgcg tcaccgatat cgtaaccgaa
     2101 cgcggctact ttgaagtcac accgcagggc ctggtcctgc gggaactggc accgggctat
     2161 accgtagaag atatccgggc ctgcacggaa gcagacttca tcgtccccga aaccatcgcc
     2221 gtcatgggcg gatgagggga ggtcgcacga aggcttcgta agcctctcct actaggagag
     2281 gtggcccaaa tgggtcggag aggtttaacc ttgtagggga tgtttttata catcacccgt
     2341 catgtataat gtgggcatca ttatttcagg cgggccgccc tttgggacgg cccctacgaa
     2401 tactgcaaac tcaatactca aaaagaggct gtcgcacatg cgacagcctc tttttcacta
     2461 ttccttatta ttgcggtcgg cttgttgtct ggccggctgc tgggtctgtt gtttggattt
     2521 ttctttggtc gtcgtttttt cggtcgtcgc cgattttttc ttcggttctt cccggtcgtc
     2581 gctgcggacg ctgttactgg aagatgaaga cgacgtcgtt tctttacggc gttccatgcc
     2641 tgacggggcg gagaagtcgc cacccgattc atcggacgta gccgacgaca tgtaatcctt
     2701 ccagatttct gccgggatcg tgccgccgta gatttcgcct aagctgtgcg aacccgtatc
     2761 gtcgccaatc catacggctg tgacgatgtc cggcgtgtag ccgacgaacc aggcgtcttt
     2821 gttgtcgtct gtcgtaccgg tcttgccggc ggccggacgg ccgatggcgg cggctgtccc
     2881 tgtgccgtgg tcgatgacgc cttcgagcat agtcgtcatt tcataggctt cccgttcgga
     2941 catgacgcgg gtcttcgttt cttctttttc taacgtcgat gcgtcttcca ggacgttacc
     3001 gtcgcggtcg aggattttga cgatagccgt cggtttgacg tggacgccct tattggcaaa
     3061 ggtgccataa gcactggcca tttccagcgg cgtgacgcct ttggtcaggc cgcccagggc
     3121 catggccagg ttgtcgtctt tggcgtcgag ggtggtaatg cccatcttct tggcattggc
     3181 gatgatgttc ttcgtgccga ctttgtcagc cagctggacg gcgatggtgt tgaccgagtg
     3241 ggccagggca tcgctgaggg tcatggtacc gctgtaggag ccgccggcgt tcttcgggct
     3301 ccagctgccg taggtgacgg gctggtcgtc catggtcgta tccggcgtca tgtcgtgctg
     3361 cagcgccgtc aggtagacga aaggcttgaa ggccgaacca ggctggcgga cagccatgct
     3421 ggcgcggttg aagctatcct gtccgcggcc gccgaccatg gccaggatgt ggcccgtctt
     3481 ggggtcgatg gagacgatgg ctgcctgcgg ctgagtcagg ccgttttcgt cagtgtagtt
     3541 gttgggcagg ttgcgcatgg cccggacggc ggcgtgctgc ttgtcgacgt ccatggtcgt
     3601 gtagatcttg aggccttcct tatagagggc atcgtcgccg tatttcttgg cgacctgctg
     3661 ggagacgtag tcgatgaagg atgcatattc gtcggcttct ttggactgat ggctcttgga
     3721 gaggcccagg tcctggttct tggcgtcttc cgcttcggca gccgatacgt agccgtattt
     3781 gaccatctgg tcgaggacga cgttcttgcg cttcttggct tcgttcaagt tgttgaaggg
     3841 cgagtagtag ttcgggctct taggcaggcc ggccagcatg gcgcactgcg tcagggtcag
     3901 ttcgttgacg tctttgttga aatacgtctt ggccgctgtc tggatgccat aggcaccctg
     3961 gccgaagtaa atctggttca tgtacatttc caggatttct tttttactgt atttgtgttc
     4021 gatttccagg gccagcatgg cttcctgtat cttacgcttc aatgtctgtt cctgggacag
     4081 gaaggcgttc ttggccagct gttgggtgat ggtactgccg ccctgggcga tgccgcggtt
     4141 ggtcaggttg gcaaagatgg cgcggaagat gccgataggg tcgatgccga tgtgttcata
     4201 gaagcggttg tcttcggcag cgatgaaggc attctgcaga ttctgcggca ctttgttgat
     4261 gtcgatgggc aagcggttct gatcggaatg aagggtcgtg atcagccggc cgtgggaatc
     4321 gaagacctgt gatgaaacgg caggccgcat gttattgcct acagcaggca aactttgata
     4381 ggcagcaaaa atatagccaa accctaagcc tgcgaaaacg acaaggcata aggcgatgaa
     4441 aaggcggatg cggcgaaggg gaccggcttt tttggctttt tttgctctgc gtctgggtgt
     4501 atacattttt tcctcctacg tgacgacgta atcgttcaag atgagcaggt aatgagacat
     4561 tactaatttc ttattatatc acatcataca tcattatcct atataggcgg gacataggag
     4621 ggacaggttg aaaaaattta aagtcttttt ggcgattaaa tcgttttacg ctacacggaa
     4681 tccgaaaatg tttcatatgt agtatactct ttaaaagagt atcgtgtagc ttttttagaa
     4741 atgccattga caatcgcaga aaaatagcgt actataaacg taacaagcag acaatttagc
     4801 atacacgctt ggttataata gcaggaggta aggattatgg caaaggacag agaaaaagca
     4861 tttgtattgt ggttcgatga actgcgcaga gccgatgtag ctctcgtagg cggtaaatcg
     4921 tcttccctcg gtgaattgac gtctcagacg gatgttcccg ttccctatgg gttcgcgacg
     4981 acagccgaag gctatcgcca tttcatgcat gaaacgggca tggacgtcaa gatcccggaa
     5041 ttactgaaag acctgaccga cgtcgaagac gctgtccagc tccgcgatgt gacggcaaag
     5101 atccgcaagg ccatctgtga tgcagaaatg ccggccgact tggcagccga tatccgcaag
     5161 agttacgaag aattgggtaa gaaagtcggc gaagccaatc ctttcgtcgc cgtccgctcc
     5221 agtgcaaccg ctgaagactt gccggacgcc agctttgccg gtcagcagga tacgtacctc
     5281 aacgtccagg gggctgacat ggtcatccag aaggtcaagg aatgttacgc atcgaccttt
     5341 accgaccgcg ctacgtacta ccgcacgaaa cagggcttcg atcatctctc cgtcgctttg
     5401 agcgccgcta tccagatgat ggtcttctcc aaagctgccg gcgtcatgtt caccgtcgac
     5461 ctggtcacgg gcgatgattc gacgattacc atcgaagggg cctggggcct cggcgaatac
     5521 gtcgtacagg gcacggttac gccggataac ttcaaagtcc gcaaagacga cctgaccatc
     5581 ctctccaaga tgatcaatac caaaccgatc gaactggtcc gcaaaccggg cggcgattgc
     5641 gaagaaaaac tcgttccgcc ggaactgcag aaacagcagg tcattacgga cgaacaggta
     5701 aaggaactgg ctacgtacgc caaagctatt gaaaagcatt acggctgcta catggatatg
     5761 gaatggggcg tcgacgaacg gaccaataag gtatggctcc tccaggcccg tccggaaacg
     5821 gtatggtcca agaagaataa agaaaagaaa gaagccgctg ccgaaaaggc cgctgtcacg
     5881 acggaccgca aagtcgtcgt caaaggcctg ccggccagcc cgggcagcgc tgctggcaaa
     5941 gcccatgtca tcctcgaccc gtcgaagatc gctgaattca aagacggtga aatcctggtt
     6001 acgaccatga ccgctccgga ctgggtaccg gccatgaaga aggccaaggc catcgtcacc
     6061 gatgccggcg gcatgacctg ccacgcttcc atcgtcagcc gtgaattggg catcccctgc
     6121 atcgtcggca cgaagagccg cagcgtcgaa gnnnnnnnnn nacaaccatc caggatggcg
     6181 cagaaatcac agtcgatgcg acgaacggca tcgtctatga aggcatcgtc gccgacatcg
     6241 tcaaaccgga agctgctgaa gccccggccg gccagactgc aacgaccgtc gtcgctgccg
     6301 aagccgctcc gattacgggc acgaagattt acatgaacct gggcaacccg gatttggctg
     6361 aaaaatacgg caaactgcct tgcgacggca tcggcctcat gcgcgaagaa ttcatctgga
     6421 cgacattcat tcatgaacac ccgttatacc tcatcgaaac gggccgggct gaccgcgtcg
     6481 tcgatcagtt gtccgaaggt atccgcaaag tctgccaggc actggctccg cgtccggtca
     6541 cgctgcgcct gagcgacttc aaatcgagtg aataccgcaa cctgacaggc ggtgacaaat
     6601 acgaacccca ggaaagcagc gccctcctcg gctggcgcgg tgcttcccgt tactatgatc
     6661 cgaaatacgt cgaagccttc aaactggaac tgaaagctgt ccgcaaagtc cgcgaagaat
     6721 acggctacaa gaacctcaac gtcatgatcc ccttctgccg ccgcgtcgat gaaatgcaga
     6781 aagtcgtcga cctcatggct caggaaggcc tccaccgcgg tccggacttc aaggtctggc
     6841 tcatggctga aatcccatcc aatatcatcc tggccgacca gttcaaccag ttcgtcgatg
     6901 gctattccat cggctccaac gacctgacca tgctcatcat gggctgcgac cgcgacaacg
     6961 atacggtagc accgctcttc gatgaacgca acctggcagt caaacgggct atccgccatc
     7021 tcattgccgt agcccataag gacggcaaga ccgtttccat ctgcggccag gcaccgagcg
     7081 tctatccgga cttcgctgaa ttcctggtca agagcggcat cgattccatc tcggtcaacc
     7141 ccgatgccgt cgtatcgaca cggaaacacg tggcacaagt cgaacagcgt atcctcctcg
     7201 attcgctgac aggcaagggc cgtgctgata cagaagatta tacgtggtaa tagcatatcc
     7261 gatttatgag taatagaaag gcggtcccaa aagaaaaaca gacttttggg ccgccttttt
     7321 ctcatactag gaccttgtat tatacactat ttgcatatta tacatcttat atcgcgggat
     7381 gtttttatat atgttatgat aataagtgaa taagagaacg cttgctatgt agggggtgaa
     7441 aagacgctat gttatcggaa cgtcaggaaa cgattttaca gattgtaaag gaaaacagtc
     7501 cgattacggg ccagcatatt gccaaccggc tccacgtgac gcgggcagcc ctgcgctcgg
     7561 acctggccat cctgaccatg ttagggctca tcgatgcgcg gccgaaatta ggctactttt
     7621 ttacgggcaa taaagaaaaa gacctgctct cgcgggccat cacggatatc accattgatt
     7681 cctgcctgtc gcagcctgtg atcgtcggtg agcatacgag tgcttacgat gcgatcatcg
     7741 ccatgtttac ggaagatgcg gggaccgtct ttgtcgggac ggacaatatc ctggccggcg
     7801 tcgtatcgcg aaaggattta ttgaaaacgg ctatgggcag caatgacctg cgggccctgc
     7861 cggtgcgcat ggtcatgacg ccagtgagca aagtgatcta tgcccagcct gaggaaaagg
     7921 ctatcgtcgc cgctcagcgc ctgatcgaat atgaaatcga ctgcctgcct gtcgtcgagg
     7981 tcgaaagtac cgatgaagac gacaagaagg aattgaagat ccttggcagg atatcaaaaa
     8041 cgaacataac ccgtctcttt gtagaatgtg ggttagggag gagataactg tggaaaaacc
     8101 gattatttat gcctgctcag actcgcttgg tgaaacggct gaacgcgtcg cccgggcagc
     8161 agccagccag tacgacaagc aagaattcaa catcatccgc gtaccgtaca tccggtcgga
     8221 acagcaaatc gaggagctgg tctgcaaggc cgaagctcac aatgccatga tctgctacac
     8281 catcatttcg ccgaccctgc gccggaaact gtgggaactg actaagtcgg ccaacgtcac
     8341 cgtcgtcgat atcatcggcc ccatgctggc cggcgtcggc accctgacgg atacggaacc
     8401 gactttgaag gccggcatga tccatcagct cgacgaagaa tatttcaaac gcgtcgaagc
     8461 tatcgaattc gccgtcaaat acgacgacgg caagaacccc atgggcttct tgaaggccga
     8521 tatcgtcatc gtcggcgtct cgcgcacgtc gaagacgccc ctttccatgt acctggccca
     8581 taagcgcatc aaagcggcca acctgccgct ggtgccggaa gtcagcctgc cgccggaact
     8641 gttccagatc ccggcccgca agatcgtcgg cctcatcatc gacccgttca agctgaattt
     8701 catccgttcg gaacggctgc gcagcatggg gctcgatgcc aacgccagct acgccaatat
     8761 cgaacgcatc aatgaagaac tggaatatgc ccgcggtgtc atgcgccgcc tccattgtcc
     8821 catcatcgat gtgtcgtcca aggctatcga agaaacggcg aacctggtca tggaaatcgt
     8881 caaacgcaat aaagaactgt atccagaata aaatcagcca atggcggcga ggggggcttg
     8941 ttgcatcatg gttcttacca ttatgcaagg gccctttttg cgtcactgcg tgaggtttga
     9001 cattacgaca tcaaacttcg gaaggagcac tatgaaagat tcgacgacag ccattttcac
     9061 gtccctgtcg gcggccctgg agagccggca ttttttggcg acgctgggaa tggaaaaaga
     9121 tgaaatgacg gcctggatac gccgcagccg cctgcggtcc ctggccgatg aattacagca
     9181 ctgccgcgac ggggaagggc gcttttcttc gcctgctgtc gtcgcggcgg ccaaacccta
     9241 tctggactgc ctccgggacg aaccggccgg cggctggctg cgctgctgcc atcagcgcgt
     9301 cctggcccgc ctctttcccg gttcctgcca gggcccggag gccatggccc tgacagggcg
     9361 cggcagtcat ggcgacatct accggcgggg ctgcctgatc ctcatgcagg tcctgtgcgg
     9421 cctctaccgc tatgaacgcc aggtcctgcc cttcgacccc aagcgggaca tgcccctggt
     9481 atcggtccag gaaatcatgg cccacggcta caccaaggaa tacctgcgcc tgcgtacact
     9541 ggctgccgac gattatattt atgaattcat gcgcatcggc gtcgacatta cgcctttcaa
     9601 tacgctgggc cacgtcggcg gcgtccacta cgtcgccgtt
//
LOCUS       DAWABZ010000064         9595 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_1946_length_9595_cov_3.088155, whole genome shotgun sequence.
ACCESSION   DAWABZ010000064 DAWABZ010000000
VERSION     DAWABZ010000064.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9595)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9595
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1946_length_9595_cov_3.088155"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5004..5013
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5912..5921
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1933 a         2554 c         2911 g         2177 t
ORIGIN      
        1 gacccctatt tatgtaaact atgttattgc acataacaca cgtgcctttt cagcccgtca
       61 ggtcatgact gtaaggaatg gcaggacggt tgctgaaacg gaccgcagta gagggggcgc
      121 ccgttggata tggggacgat ttctccagaa aactgattgg gaaagcacac atattctgga
      181 gcgggcttgc tgcagcaagc ccctacaatt ccagggacta gcgtagctag tttttagttg
      241 ctaaggcaat gcggaccagg gtcagcataa tcgggacttc ggtcaggaca ccgacggtcg
      301 tcgccaaggc tgccgggctg gtgacgccaa agagcgcaat ggctacggca acggccaatt
      361 caaagaagtt agaagcgccg atgaacccgg ccggtgcggc aatgtcgaag ggcagtttca
      421 gcagccggca ggctccatag gcaaggaaga agataaagaa ggtctgcaag gtaaggggaa
      481 tggcgatgag gacgatgtgg agtggattcg ataagatgac ctgggcttgg gagctgaaaa
      541 tgatgaccag cgtcagcaac agtccccacg tcgttgccga atcgaatttg tgtacaaagt
      601 gctgttcaaa ataatcgaga ccttttcgtt tgacgacagt catccgggtc aggataccgc
      661 ctgccagagg aatgacgacg aacaggaaga tactgacaaa gaggacacta taaggaaccg
      721 caacttgtga aactcctaaa aggaacttga cgataggtac gaaagcgatg agaatgatta
      781 aatcattcgt cgcgacttgg acgacggtat aggccggatt ccctttgacc agtgtactcc
      841 agacgaaaac catggccgta cagggagccg caccgagcaa tacggcgcca gccaagtatt
      901 gccggcccag gtccgcaggg atccacgaac caaagaggac gaaaaggaag aaccaggcaa
      961 tagcatacat ggtaaaaggc ttgatgagcc agttggcaat ccaagtgacg aacaagcctt
     1021 taggctggcg tcctacggct ttgatgctct gaaagtcgac tttcatcatc atcggataaa
     1081 tcatgagcca aatcaaaatg gcaatgggaa tagaaatccc catgacctgt aaggtatcta
     1141 aaaaggctgg cacagccgga agggcataac cgatggctac gccgacgacc atgcataaca
     1201 agacccaaag ggtcagatat cgctgaaaaa aactgatgcc tgcgtgtgat tcatgttcca
     1261 tgataacgcg cctcctataa atagataaat tgaaatatat gaatgtttaa tatctgaaaa
     1321 agggcttgct acacgatgac aagccctttt actacaaact acaaacgctc aactacagac
     1381 tataattttc ccaggacttc cccgcggact tcgtcggggc gccaggcgat gagggccgtg
     1441 tggccctggg tgtggtcgtc gactttgttg atccaggcga tttcgtggga cgctttggct
     1501 tttagcaggg ggctcgtcgt ccgggcctga tagaaggatt tgttgatgac ccaccatttc
     1561 gagcgcagtt tcgtccgctt caggtccgct tccaggcgca gggcttcata gaccggcgtc
     1621 gcttcgggca gggtgacgat gacgacttct gtttcgtccc ctttcaggcg cggcagcagg
     1681 tgctgtactg acggcggcgt cgaaccgtgg gtccggcgga tttcccggtc gtagctttcc
     1741 gtcgattcca ggagcagcag cgtgtggcct gtcggcgccg tgtcgatgac gacgatctgg
     1801 tcgtcggccg cttcgacgat gtcggcaaag gcatggaaga cggcaatttc ctgggtgcac
     1861 ggcgagcgca ggtcttcttc gatgtaggcc aggtccgacg ggcccaggcc gttggctttg
     1921 gcctggctca ggacttcatc ctggtatttc ttcagctctt ccccttggtc gatatggctc
     1981 agggtcaggt tgtccgtctg gctgacgacg tactgcaagt gagcggccgg gtcggtcgtc
     2041 gtcaagtgga cgcgatggcc gcggcgggcc aggcccaggg cgatggcggc agcgacggtc
     2101 gtcttgccga cgccgccctt gcccatggtg aagatgacct tgcgctgatg ggcttccagg
     2161 tcgtcgatga cggcctgcag gggctggacc cgttccagcg tcagttccgt cgccgcttcc
     2221 ttcacttccc ctttgagcag ggcccggacg tggtccaggc cggtgatgtt gtacgtccgc
     2281 aagggaatct ggaagcgcgg caaatcggcg atggcttcgg gtatggcctt cagggcggcc
     2341 tgctgttttt cgtataacgt cttggtcgca tcgtcatccc acgaggccaa caggccgttg
     2401 acgatgagct gctggttggc aatgcccagg gcggccagtt ccttcgacgc ccgggccgct
     2461 tctttcagcg gcagggcatc gggacgggcg acgaggagca tcgtcgtcct gtccccgtcg
     2521 gccagggtct ggacggcctg tttatacagt tctttccgcg attccaggcc cgacagctgg
     2581 cccaggcagg acgcgccgtg tttgctcttc tggatgaact ggctccacgc cgacggcagc
     2641 tgcagcatgc gcagggtgtg gcctgtcggc gccgtatcga agatgatgtg gtcgtagcca
     2701 caggcaacgg cttcatcggt gaggaaattg gtgaactcgt tgaaggcggc gatttctacg
     2761 gtacacgagc cggacagctg ttcttccatg ttgcgcaggg ccgcttccgg caggaggcag
     2821 cgatagggcc cgatgacgct ttcccggtat tcggccgcgg cctggaccgg gtcgaggttg
     2881 gctacggcca agttgggcac ggcggcgatg ggcgtggcct tattggtcag ttccatggaa
     2941 aagacgtctt gcagattcga cgccgggtcg gtactgatga gcaggacctt cttaccggca
     3001 tcggccaggg cgacggccgt ggcacaggcg acggacgtct tgccgacgcc gcccttgccg
     3061 gtcaggaaga ggtatttcgt ctggacgacg tgaaagggat cgtagaggat catttacagt
     3121 cacctccgca acagcacgac gggctttccg gttccgccgg cgtgtcaccg cagcagcagg
     3181 aagctgtttc ttcggcgctg tcgccgcagc agcagctgtc cccagcggcc tgagcggctt
     3241 cgaggatggc cgggtcgaag ccgatgaggc tggcaaattc atcattcgtc ggatagcggc
     3301 cggcaaactg gacggcgccg tcgacgagga tgaggggcag gtcttcagga ccgtggtact
     3361 ggagaaagtc cgtcacggcc ttggtctgga cgaatttcaa gggcgcacta gtcaggttat
     3421 agcggccgac gtcgatgccg tgctgtttca gcgtgtccag gaccgtcgat acgcgcaaga
     3481 gttccggatc gatagacgga ccgcagagac cggtcgggca gcacattgct tgttcaaaga
     3541 tttccatatg tttcatgatg aaagacctcc taaatagaaa tttatatatt tacatttatc
     3601 aatgtataag ctgaaaaaag ggcctgcctc actggcagcg gcaggcttcg tcctctgccg
     3661 acacggcact gggctggaat aagtcgtgaa cggcctgttc caggacctgg gcctgggcgc
     3721 ggttgaggga ataatagacc cacttccctt ccttgcggcc tgtgaccagc tgggcatcgc
     3781 agaggatctt catgtggtgg gacagcgtcg gctgggacag gttcaagtgt tccaggagca
     3841 cgcaggcaca gcgttccccc tgccgcaaga gcaacaagat gcgcagccgc ttttcatcac
     3901 ccaaagcctt gaatacgcga accgcttccc tttcctccat cacggcgcct cctttcatat
     3961 cgaagtttat caatctatgg ttagagtata gcagacacat tgacgtttgt caatgtgtct
     4021 gctagcggct cgtggttcgt aaaccaatcg cgttcatttg ggaaagccat tcacgggatt
     4081 cccacaagcg ggccgcctag ccacagctgt ccgttttggc aaccgtcctg cgcctctcgt
     4141 tggtcggcac ctgacgggcc aaaaaggctt aggacggccc ctacgaaagc ggtccatagg
     4201 accgcgccac gaaaaaaagc ttgtcgcaca tgcgacaagc ttttttcatc tctctatttg
     4261 cttaccaagt cttcttctac ctggatgtgg agttcatgca gctgtttttt ggtggctgtc
     4321 gacggagctt cgctcatggg gtcgatagcg ttctgggtct tcgggaaagc gatgacgtcg
     4381 cggatggact tgcgtttgag catgagcatg atcatgcggt ccaagccgaa ggccaggcct
     4441 ccgtggggcg gtgcaccgta ttcgaaggcg cggaggagga agccgaattt ctgttctgct
     4501 tcttccggtg tcaggccgat ggccttgaag acgcgctgct gtacgtcagg ctggtagata
     4561 cggagggagc cgccgccgag ttcgatgccg ttgaggacca agtcataagc cttggcatag
     4621 acgtgagccg ggtcggtttc cagtttgtcc aggtcttcgt cgcgcatggc cgtgaacgga
     4681 tggtgcatag cgacgtagcg gtgttcttct tcgctgtatt cgaacatggg gaagtcgacg
     4741 acccaggtga aggcgaattc gtcttcgtcg atgaggttca tgcggcgggc catttcttta
     4801 cgcagttcgc cgagagcggc tgcgacgact ttcggtttgt cggcaatcat gaggaccagg
     4861 tcgccctttt cagcaccgca ggctttaccg atttcacgga gcttgtcttc gccaaagaac
     4921 ttcttgatct gcgatttgag cgtgccgtct tcgaggaaac cgatccaagc caggcccttg
     4981 gcgccgtagt ggctgacgta ttcnnnnnnn nnnacgacgg ggaatgtcgg cgtcgccttt
     5041 gacgttgatg gctttgacct gaccgccgtt gtcgatgacg ctgcggaaga ctttgaagtc
     5101 cgtgtccttg acgagttccg tgacgtccgt gaacttcatg tcgaagcgca gatccggctt
     5161 atcggagccg tagttttcca tagcgtagtc ccaggtcatg cggtgaatcg ggagcttgac
     5221 gtcgtggccc agggtttctt tgaagatgta atgtaccatc tgttcgacga gggtcaggat
     5281 ttcttcctgg tccatgaagg acatttccat gtcgagctgg gtgaattccg gctggcggtc
     5341 ggcgcgcaaa tcttcatcgc gaaagcagcg ggcgatctgg tagtaacggt ccataccgga
     5401 aatcatgagc agctgtttga aaatctgcgg cgactgcggc agggcgtaga atttgccttc
     5461 gttgacacgg ctcggtacga ggtagtcacg ggcgccttcc ggcgtgctct tgcagagttc
     5521 cggcgtttcg atgtcgatga agccgtgttc gttgaggaac gtgcgcatgg catgttttac
     5581 tttatggcgc atgatgaggt tcttctgcat ttccggacgg cgcaggtcga ggtagcggta
     5641 tttgaggcgg atcttttcgt cgacgtcgat gttgtcttca atatagaacg gcggcgtctt
     5701 ggacgaattg aggatacgca gttcttcgca gcggatttcg atggtcccgg tcttcatctt
     5761 ggggttgatc gtatctttat cgcgcatgcg gacgatgccg cgtacagcca ggacgtattc
     5821 cgtacgtacc tgttcagctt tatcgaaaga aaccttttca tagtccggcg aagcgacgac
     5881 ctgtacgatg cccgtacggt cgcggaggtc gnnnnnnnnn ncggaggtcg atgaaaatca
     5941 gtccgccgtg gtcgcggcgg cgttcgaccc agccactcaa gacgacttct ttgccgttgt
     6001 ccgcttcact tacttcagcg cagtattggg agcggtgtaa accttccatt gtatccattc
     6061 gtaaatcatc ccttcttgtg tgtttccata tatgcaacaa tcccatcaag gggaacgctg
     6121 acttgttctt tgctggccat attgcggacc tgggcttcac cggcggctaa ttcgttttcg
     6181 ccgatgatga ccgtgtaatc ggcattgagc ttgttggccg atttcatctg gcccttcatg
     6241 ctgcggccca ggaggtcgat ttcagcatag aggccctttt cgcggagctg ctgctgcagt
     6301 tcgaaggccc tggtcttggc cgcatcgcca agggcgacga tgaagacggg ctgcttcttt
     6361 tcgaccggcg gcaggaggcc ctgttccttc aaggtcagga gcagccgttc catgccgatg
     6421 gcaaagccga tgcccggtgt cgacggaccg ccgacttctt cgatgaggcc gtcgtaacgg
     6481 ccgccaccgc agaccgtgct ctgtgcgccc aaaggagcgt acatgatttc aaaggccgta
     6541 ttggtataat aatcgaggcc gcggaccagg cgcgggtcga gggtaaaggg aataccgatg
     6601 gccgtcagat attcctggac tttgtggaaa tgttccttgc agtcgtcgca gagattatcc
     6661 gtgatcaaag gtacgcccat ctgcatcatc ttttcgccgt cttccttgca gtcgaggacg
     6721 cggagagggt tcttttccag gcgttcgttg cagtcgtcgc agagctggtc ccgtttgtcg
     6781 gcaaagaaat cgaggagctt ctggcgatag acgggacggc acttcgggca gccgacggag
     6841 ttgaggtgca ggaccagatc attgaggccc agttcccgga agagctggaa ggccatggca
     6901 atgatttccg catcgacagc cgggtccttg gagccgattt cttcgacgcc gaactgggtg
     6961 aactggcggt aacggccagc ctgggggcgg tcatgacgga acatggggcc catgtagtag
     7021 aacttgtgga cctgcgggtc gccgtagagc ttgtgttcca ggaaggcccg gacgacagaa
     7081 gccgtatttt ccgggcgcaa tgtcatgctg cggccgccgc ggtcttcaaa ggtatacatt
     7141 tccttggtga cgacgtctgt cgtttccccg atgccgcgta aaaacaattc agtactttca
     7201 aacataggcg tgcggatttc gccgaagccg tagagcgagc agatctggcg gatctttgct
     7261 tccagcagct gccactctgc cgtctgttcc ggcagaaaat cctgcgtgcc tctcggtgca
     7321 gtaatagcca tttaaacatc ctcttttccg tatatgatag cacacaaacg gcggcgctgg
     7381 gctattcttt cggccagcgc ctgcacatat gcatcgacgt ctttcagcat gtacaccttt
     7441 tccaaggtgt ggccgtcgcg gcagaggaac ttcgtcgccc cgcccggtcc ggctgccagg
     7501 acggactgcc gttcttccat catctcgata ttataggcac tgacgctgcc cggcagggca
     7561 tagccaatgt tggcgaagct ggctgccatg tacttctggc ggtacatgta gtacggcaca
     7621 tagccgccgg ccgtcaggat ggactgacag aggcggacca tatggcccgt ttcttcggct
     7681 ggcggaatgg cggcccgcaa ttcatgatgg aataagggtg cccgtttttt taatgctaac
     7741 gtatgaattg taacattttc cgggtgcagt ttgcaaacaa tttccatatt ttcctgcata
     7801 tctgccacgg tttgcccggg aaggccggcg ataaagtcca tgttgatgac ggaaaaaccc
     7861 agtttccggc agttctcata catggtgtaa atatcttcga cccggtggcg ccggcccagg
     7921 gcatcgagga gatgctgctg caacgtctgg ggattgacgc tgatgcgatc gacaccggcc
     7981 tggcggagca tggccagcat gtccggcgtc gccgtatcgg gccgtcccgc ttcgacggtc
     8041 cattcgcagg cttccgggaa attccgggcc agccgcgtca tcatcgcttc cagtaccggc
     8101 aggggcaggc acgtcggcgt accgccgccg acgtagagcg acgtgacttc caagtcatag
     8161 gtccggcaca ggtcggccag gtcgtcgagg tcggccagga cggcatcgcg aaaagccgtg
     8221 agttccgcct ccccttccct gccgatgagg cgcgacggga aggagcagta caggcagtgg
     8281 ctgggacaat agggaatccc tacgtagagg gccactttcc gacccctgtc ggtgacgtaa
     8341 ggccgttgga gcctggccat ggccaccagg tcccgggcga cggccggcgc cacgcaatac
     8401 gacgtctgca aggccttttc ggcctggtcg gctgtgaagc cctggtcgaa gaggtggtgg
     8461 accagtttcg tcggccgcac gccgatgagc gtcccccacg ggctgggtcc gtggccgcag
     8521 aagcggttat gccagtggat gaggcagtcc ttgagggatg cccgttcccg gtccgtcccg
     8581 acgaggcggg cctcagaaga ccagggcgcc ccgccgtcct ggaaggtaca gcgcacgtgg
     8641 aggtgggcgc cttcctcgcg gacagtgacg gcagccccga cgtcgcggac ttcccggccg
     8701 ccgacatagc ccagggaggc catgacctgc ccgacgaggg aatggaagcg ttccggcccg
     8761 tcataggtat aggtctggag ggccatctta ttccgcttct ttcgcagctt cggcttcttt
     8821 ggccgcttct tcttcccgtt tcttctggca ggcttcgcag tagccataga ctttcagctg
     8881 atggtcgacg gtgtggaagt ggaggcgctt ttccaggatc gtttccagcg tttccaggag
     8941 gtcgtcttca cattcccaga ccttgccgca gcccaggcag atgagatgat gatggaaatg
     9001 gccttcattg tcgcggtcat tcaattcata gcggctgcag ccgtcgtcga aatcgagttt
     9061 cttcaagagg tccatctggg tgaacaggtc cagcgtccgg tagaccgtag ccaggccgat
     9121 gtcaggcgcc gtcttcttga ccagctcgaa gacttcttcg gcgctcatat ggcggatcgg
     9181 gctttcgacg aaggcccgca ggatgatctg ccgctgggac gtcatcttgc atttcttgaa
     9241 cttgatcttt tctttcatgc gtttcatcat ggtatccatg atttctttct tattggtttc
     9301 tgttctcgtt gtttccattg tacttcatcg ccttttcttt atatgtattg taaaaccata
     9361 aatgtttaca caaaatctgt aagactgccg tcaccgggac ggtaaggatc atgccggcta
     9421 cgccaaagag ggtcccgccg atgagcaggg cgatgacgat gacgaccgga tgcaggctga
     9481 tgacggcccc catgacctga ggcatgatga ggtggctgtc gatctgctgg atgacgatat
     9541 agaagatgat gactttcagc ggcagggaca aactgaccgt cgcgttgagc aggat
//
LOCUS       DAWABZ010000065         9325 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2007_length_9325_cov_3.588673, whole genome shotgun sequence.
ACCESSION   DAWABZ010000065 DAWABZ010000000
VERSION     DAWABZ010000065.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 9325)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9325)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9325
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2007_length_9325_cov_3.588673"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5174..5183
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2048 a         2490 c         2499 g         2278 t
ORIGIN      
        1 ccgcaaaggc cagggcctgg cggttcatgt gttccacctg gtctgggctc tggcgccacg
       61 attccagttt attgatgacg taattagctg cgaccttgca gacccgcttc tgccgttccg
      121 ggtcctggac gtagcgctgc caggcttctt tcgtgtccgg ccgcgtgacc aggctgttgt
      181 acatgcggtt cttatagctg tcgatgcgct ggcgccattc gtcgttgaat tcgatctttt
      241 ccgtctgttt ccacaaatag cgcagggcct gctggcgctg gagggaattg tcttccttgg
      301 cttcggccag gaggtccagg cacttcgtct gcaagaggcc ggctacgtgg tccggctgga
      361 agacttccat cttcaaggcc gccttgatgg cccagccgag gaagatattg tgcttggcgt
      421 actggcgcag ggaggcttcg tagatttcgc cgacatagcg cttcatcgtt tcactgcgca
      481 cgaggtcttc caggttgagg atcatgaaat cgaggaaatc cttccccgat tcaccgcgca
      541 ggccgatttt gatggccttg ctcatgatcg gtgccaggtc gactttctcg atagcctggc
      601 cgccgatgcc ggcaaaggcc ttgacgacgg tcttcatgtc gatggtgtac aagaaggtat
      661 tgaggatctg accgaagacg cgttctgccg actggaaccc cttttccgta tggagataat
      721 ccagcgtcgc gcccaggatg ggatgatgtt tgaggacgtt gtacatattg gaaacggtca
      781 ggatttcata ttcgatcata tgccgggcca tttcggcaat gcgttccttg ctgttcggaa
      841 tgagggccgt cttaaaggag atgcccaaag gccggcggaa cagggccgtt acggcatacc
      901 agtctgccag gccgccgatg agggccgccc cggccagatg ggtcagcagt ccgccgacaa
      961 aaaagtcttg gaaggggtat gccagaagca ggaggactgc cgccgtaatc aggatataat
     1021 tggcaatctg gctgcgtctg cccccgtgtg tattacttgc catgatacct ccttactgcc
     1081 cgccgatgat gacgtcgtcg ccatcatgct ggggaatcca caagggtacg tccggataag
     1141 cctgtttaaa agctgccgac ggcgtccagg gcgtgccgaa ataatgcatg gggatgaggc
     1201 atttcgtgac cgaaacgtct ttcaggaaac ccgtgacgcc ccattcgcgg gcgccttcca
     1261 ggcgggcgtc gacagggaaa aagacaatgt cgaaagtctg tccggcaagg tgtttcattt
     1321 cccggtcgaa gttgcccttg gcttcggcat tattttcatc cgtatcgccg agccagtgcc
     1381 accagttcaa gtcgccggca tggaaaatct tcaagccgtc cgtttccacc aggaacgacc
     1441 cgccttcatc agtcgaccca tactgggtca ccgtcgtatc tcccaggggc agcgtttcat
     1501 acaacttcat ggcatggaac cggtctgccg gaatgtcctt gaggcgcaca tcttcattga
     1561 cgatgtactg ggcaccgccg aactggccga tgctgcggtt gaaatggtct tcgtgccaat
     1621 ggctgacaaa gaaccacaac ggcttcctgc cgacctggct gtaagccttg accgtccccg
     1681 atggatcctt gtagtaatca aagaccagca ccttggaatc ggtctcgacg gcaaagccgc
     1741 tgtgattcaa aaaggtaatc tgtacactca tatagtaacc cccttatcgg ttgtttaatg
     1801 gctattgcat actttttatt ataccatata tgcgtcgccc gtgtacatgc cggggagggt
     1861 tgtggctagg ggctggtggc tcgtagttcg tggctcgtgc gaatttctcc aatttttagt
     1921 tgtaggggct cgcacgtgcc ttttcagccc gtcaggccat gactacaagg aatggcagga
     1981 cggttgctga aacggaccac tgtagagggc gagcccgctt gaggatatat gtgattcccc
     2041 cagccagttt gctagggaac tcatccaaag ttctaacggg tcgcccacgt ggcgacccct
     2101 acaaagtaaa aaggcttgcc gcacatgcga caagcctttt ttactttccc tctgcgatca
     2161 gccctgatgg aattctacgc ccatagcgcc tttcggatgt ttccagctct ttacgccttt
     2221 gccgccggaa atgacgcggc acgtaccaca ttcgaggcag ccttcatgac tgaagaccag
     2281 tttgccgtca tcatagtgat acaggttagc cggacatgcc atgactaagc gtttgatttc
     2341 tgcttcgtca tgataatctt cgttgatctg gatatgagat tcgtgttcat cggtcacgaa
     2401 cttatcgaaa cccagtttat cgtctacact catctttgcc atcttacatc gcctcccaag
     2461 ctttcttacc taaatcgaag agattagcca agccgccgtt gtttgccaaa actgtcagag
     2521 caatcatggg cagagcgacc ggtgcgccgc cgtctacttt gtagaccatg ccggcaaatt
     2581 cgtcggccat cttcgggaaa tcgttgaaga taatgtgatc ttccaagagg cccggcaggt
     2641 tctgatactg tttcatgagc ttcatgatga agctttcttc taacaaggtc ttgtaaaccg
     2701 agaggctttc agccgagaag tcgttgcggt tcttcgcttt gatgacggct tcgccagcca
     2761 gacggcccga ttcgatagcc aggtccatgc cgcggacggt gtaacccagg ttaacgacca
     2821 tagctgctgc atcgccagcg acgaggacgc cgtcacggac gagttccggc atcatcttga
     2881 ggccgccttc ggtaacgagg tgagccgaat attctttcag tttcccgcct ttgatgaagg
     2941 gagcaatagc cggatgagct ttcatgcgtt caaccatgtc cggtacgctg acatcgacgc
     3001 ggccgatatc gctgatggtc gtaacgatgc ccagggacag gctgtctttg ttggtgtaaa
     3061 tgaagccgcc gccgaggttg ccgcccgtcg ggtcgccagc catcatccag gctgcgccgt
     3121 cgttgccgct caaaccgaag cgttcgttga tgacgtcttc accgagttcg atgacttctt
     3181 tgacgccgac tgcaacgtct ttctgttcca gttctttctt catgccgatc tgctgtgcca
     3241 gcagggagtt gacgccatca gccaggatga cgacatcagc gtcgaattct tcgccgcccg
     3301 tcatgacacc gatgactttg ccgtcttcga cgaccagttt atcgacgagg aagccttctg
     3361 tcagcatgac gccggcttct tcggctttat cggccaacca tttatcgaat ttagaacgca
     3421 gtaccgagta ggacggatat ttgaggtttt ccagtttatc cgaaccatac tggaagctca
     3481 gtgcgccgcc ttctgtcatg agcgacaggc gttcctgcgt aatcttacgt tcaatcggag
     3541 cttctgctgc gaaattcgga atcagttttt ccaagctgtg tccatagaga cggccgccgc
     3601 tggagttctt ggaaccgcag aatttaccgc gctccaccat cataacatcc aaaccagctc
     3661 tggccattgt caaggctgca gcagaaccag ctaaaccgcc gccaaccacg atggcatcaa
     3721 atttttcttc actactcatg ttcttaccct ccttaaccat acggtttgct atcttaattt
     3781 ccgttcgtat ctatcgtaac gtaatgtaag tccttttcat aatccttcat tcgaccgcag
     3841 gcttcttcca ggagctcctt cttcctgaga aaatcctgcg tcgactgcca cagccaggtg
     3901 tattcctgcc gatggcggaa gccgccgccc aggaagggca tgacataccg gaagtacacc
     3961 gaataaaagg gccggatgac cagagaatcg gggacaaagg agtcgagaca atatacccag
     4021 ccgccgggaa cgacgacgcg ggtcatttcc tgcaggacgc gcaggtagtc cgatgtgttg
     4081 cgaagcccga aggagataca ggctgccgaa aaggtattat cctcaaaagg caggttcatg
     4141 gcatcacctt cctgccaggt aacgttagca agcggcgcgc cttttttgcg ggctacgtcg
     4201 agcatcgccg gcgaaaaatc gaggccagtg accttgagat ccggccggtg cgctgccagg
     4261 gaaatagcga tgtccccagt gccactgcac acatccagga cacggctgcc cttggctgcc
     4321 tgctcacaga cagcttcgat gaggcagtct ttccaacggc tttccatgcc gagactgatg
     4381 cgcccgttgg cgcggtcata agtctgggaa atctgctgga atacatgata taccttctcc
     4441 cgttttgaga gttcagccaa aagtaccacc tccgaagagg accatcgcga gtgctaacag
     4501 ataggcaccg ttgccaaaga gattggcggc gatgatacct ttcatctgtt tcacccgccc
     4561 ctgcggttcc agaggcgact gaagcagatt gacaaaggta tgtcgggcaa acacgataaa
     4621 gaaagccggg ctgatgaggc cccaccaacc gatataccat acgggcagga ggcagatgag
     4681 tgccatccag atccatacgg cagcccggta caggcggacg gcctgagtcc ttcccaagag
     4741 tgccggtaag gtccggcgct tggcctgcct gtccttttca atatcactcg tattattggt
     4801 catcatgatg agaccgatac tgatgatgaa gggcagagcc gcaggcaaaa cggaaaaatc
     4861 gaaggtactg ctgacggcgg cgacgattcc catggggatg aggccgccca tgacaaagcc
     4921 cgaaacgaac tcgccaatcg gcagatagga tatcggcagg ataccaccgg aataggtcca
     4981 tacggcgatg gctccgatga cgccgatgat cagcggcgcc ggtccggctg tgaccagtgc
     5041 cggcaaggcc aggagaccgg ctgcaactaa gtagccaagt cccaaagcca atgcctgtcc
     5101 aggcttgatg ttttcataaa ccaggacggc gtcgctcacc tcgacgttgt cgtcggcagt
     5161 gtccgtgcct tttnnnnnnn nnnatgaaga cgcaggccca aaagagggtg atggccagcg
     5221 tccccgtaag ggggtagccc ttcaggaggc aatacagttc accgaatacg gcgggatata
     5281 cagaagccag ccaggtgtgc ggtgcagcca ggcgaacggc cagcttaggt gttaagcaat
     5341 gttgctgttc cattcacacg gcccccagct tacgtacgat cttgccgagc tgcagggaaa
     5401 tatcacttga cggcagggct tcgaggatgt cctcagcccg tttctggtag gtgtggatct
     5461 ggttccacgc gccttccagg ccgcccaggg ctgcgacgat ttcttccatg cggcagatat
     5521 ccgcatgagt cagcgtccct tcggcgctcc tttccatata ggggtagagg agccggcggc
     5581 cgccgggctg ttcccgtgcc aggaggaccg gcagggtata gatcccttcg cggaaatcct
     5641 ggtgggcttt cttgccaatc atcgaactat cgggtacgaa gtcgaggagg tcgtcgcgca
     5701 tctggaacat atagcccaga cattcgccga aggactcgag catccgcgat acccgttcgt
     5761 cacaaccgct ttccatggca ccgatgcggc agcaggcgcg gaacaaggcg acggtcttgc
     5821 cgtggatgtt ctgcagatat tggtcaatag tgacgtcctc tttatagcgg cacatggcct
     5881 ggccgatttc gccggcacac atttcctcta cagcctgggc gatgaccata cctgcccggt
     5941 tcaatccctc gcgcatcaag tagaaggtga tgcggctcat caggaaatcg ccggcataga
     6001 cggctgcgtt cttcccgtac ttgctctgca ccgacggctt gccgcgccgg accggtgcat
     6061 cgtcgacaat atcgtcatgg atcagtgagg ccagatgggt catttccact acggccgcca
     6121 gtttatacaa ccgctcccgc acatcgtcgc ggtctgttcc gaacgagccg gccatcagta
     6181 ccaggcgggg acggatcatc ttcccctgcg aagaggctgc atcgtcgagg acagcgccag
     6241 tggctgtacc ttcaaaggga tgctgcgtat atagttttat atactctttc actctgcgga
     6301 cctggtcatc gctgataccg gcaaacaatt tgggcataaa cttccgggcg ctggtttcta
     6361 agagtgtctg ttttatcatc atgctctatt tacctttccg gccaacgtta ttgcctattt
     6421 tttttcgcgt ttttcaacga cgatctgtgc tgtgtgttta acctgaatgt tgttcaagcc
     6481 gcgagcatcg aggccgccac ggatctggag caagcagccc gggcagtcga cagcgacgac
     6541 atctgcgcca gaagcgacga tgttgttgat cttttcttcg aggatcggag ccgacatttc
     6601 agggaattta acggagtaag aaccaccgaa gccgcagcat ttatcgcaat cgtgcatttc
     6661 gatgagttcg acgcccttag tagctttcaa gagttcacgc tgttccttgg tgacgccgag
     6721 gtaacggttc aagtggcagg agtcgtgata ggtgaccttc atcggtacgc catcgccggt
     6781 atccggcaag ccgccgagca tggaaacgag tttgcagaag tcgaaggtct tgctgcggag
     6841 tgcttccgct ttcttgagca tttccggatc gtccttgaag aagtcgacat aatcgcggag
     6901 ggcatgcgta caagtcgggc aagccgatac gatgtaatcg tatttttcag cttccatagc
     6961 gttcaagttc atttcagcag ctttcttggc gttttcaaca tcgcccatat aggtagccgg
     7021 agcaccgcag caagcctggc caagcggcat ttctacgata tagccagcca tgttgagggc
     7081 tttgacgacg tccgttgcga tgtcgacata gacgaagtcg aggagacagc ccgtgaagat
     7141 agcgatcttg cccttcggat tgggaacatc ctgtttgatt gtcggcagga tatcgcggaa
     7201 cggctgcgga gcaacgctcg gcagactgcg gcctgctgtc agaccggaca agaacatcgg
     7261 cagatgacgg atcatcggct gacctttggt aatcatgccc tgagcaacgg aagcgatgcg
     7321 cagcatagag tggaagaggt gacggtcagc aaccgtatcg agacagaatt tatggaccgg
     7381 attgaggcca tcttcctgag cgaatttcgt acgtaatttc aggatcatgc cggcaatatc
     7441 gagtttgccg ccgcagactg tgttacacgt accgcactgg agacagatgt tttggatatc
     7501 tttaccgcgt tcacggctgt tgaggaagct cgtgagcagc gtgccgatac cgccggtata
     7561 aatggagccg ccatatacgt ggccgccgac gaggcggaag gccggacata cattcaggca
     7621 ggcagcgcag cggatgcagc agaagatatc tttgtaatct tcactggcca tgatcttacg
     7681 gcgttccggc gtatcgagga tgatcatgtg gaagttcttg tcttccttcg tatcgttttc
     7741 cggatccgta acgactttcg tagcgcccgt gtagaaggaa aggtatgccg tgatgttctg
     7801 ggctgtaccg ttacgaggca gtactttgaa gatgtagcgg atatcgctca tcttagcgac
     7861 gaatttttca ataccaaaga tatataagtg cgttttcgga agggttgcga ccatacggcc
     7921 gttgccttca ttcgtcatgg taatgactgt ccccgtttca gcgacagcga cgttggcacc
     7981 ggaaaccccc atttcagcgc tgaggaattt cggacgcatg acgcggcggg acgtcttgac
     8041 ttctttcgtg atgaccggtt cttccggctg tttcgtatac gattcaaaga ggtcggcaac
     8101 gtcttcttta gaataatgaa tagccggcat aaccatgtgg gacggcgtat caccagcgat
     8161 ggagttgatg aattcgccca agtccgtttc ctgagccgta atgcctgctt tttcatagtt
     8221 ctggttgaag tgcatttctt ccgatgccat ggatttcgac ttaacgcaaa gtttgacgtt
     8281 gttgtctttt gccagtttca aagcatagtc catagcttct tccggagaat gagcgacgaa
     8341 tacgtgagcc ccgttggctt ccgcattctt gatgaattcg gccatgactt cgtccagatg
     8401 gtcacgagca taggatttta cttcatggac tttctcacga agcgcttcaa agtcatatcc
     8461 tttgtaaact cttgcacggt tgccaggata agctgtcgta aagttcttca acgccttctg
     8521 cagcggcgca ttatccatgg cgcgctcgat ttcttgcttc agtaattcgt tagacatgtg
     8581 ctgttccctc ccttacgcat cttctacgac gatgaccgaa tactgacgcg ggccatgaac
     8641 ccctacagta ccgacacatt cgatatcagc tgtccggctc gggccggtga tgataccggc
     8701 gaatttcggg aacggtttcg tttcggccat gatgtcgatc atgtcatcga aagtatcgac
     8761 gatcttagaa agtttgacga tgccgacata gacgtccgat accgtttcaa cgatacgaca
     8821 gtcgatgtcg tccttcatct ggacaatgga gcccaagttg gcgatgccgt aatcggcttc
     8881 ggtaacgccg cctttgtcgt ccggggcatt cttgcggaag tgatccgtat ccactttgat
     8941 gcctgcggct ttcaatgcgt ctacagcgcc gatttctttc aggagcggcg tttcaacgag
     9001 ggcaacttca tgtgtgtcct tgtcctggaa aattttggtg agagttgcag caacgtctgc
     9061 ttttttggcg atgtaacttt cgccgttgac gctggtcaac cctttctgga atgtttcata
     9121 caattctgtc ttttccattt tcacacctct ttacttataa atcacaacta caacgaatac
     9181 tatgaagtaa agtatttaac ataagtatac catttcatgc gcaaagtgca ataagtatag
     9241 aaaaatgggt aatacaaaat gagaaacctt gaatggtcat ctatgcattt ttgttattag
     9301 ctcattcatt actaatatgg tcttc
//
LOCUS       DAWABZ010000066         9313 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2012_length_9313_cov_3.014690, whole genome shotgun sequence.
ACCESSION   DAWABZ010000066 DAWABZ010000000
VERSION     DAWABZ010000066.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 9313)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9313)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9313
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2012_length_9313_cov_3.014690"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    951..960
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2002 a         2644 c         2660 g         1997 t
ORIGIN      
        1 gccgtggacc aaggccggcg tatgttcgat ggtctggacc aaattcggct tgatggcatc
       61 tttcatgagg atggccatgg cgccttcgac gccgagctga cgggctttga ccagattgcc
      121 gtccaggtca taggccacgg tgatgtcgcc catgcgcttc ttgaggtcca tgaggtcgct
      181 ggccaggcag agggctgcca tgatttccga agcgacagtg atcatgaaat ggtcttcccg
      241 cgggaaaccg cagactttgc cgcccatgcc gacgacgacg ttgcgcaaag cccggtcgtt
      301 catatccatg acgcgtttcc agatgatgcg gcgcacatcg atgcgcaggg cgttgccctg
      361 gtggacgtgg ttgtcgatgg ctgcggccaa gaggttattg gctgccgtaa tggcgtgcat
      421 gtcaccggtg aagtgcaggt tgatgtcttc catgggcagg ccctgggaat agccgccgcc
      481 agcagcaccg cctttgatgc cgaagaccgg ccccagggac ggttcgcgca gggcgacgac
      541 agccttatgg cccatgtagt tcagtgcctg ggccaggccg atagtggtca aggtcttacc
      601 ttcgccagcc ggtgtcggcg tgatggccgt gaccaggacc agtttcccgt cttcattctt
      661 cgacaggcgc ttgatggcgt caaaagaaat ctttgcttta tattttccgt agagttccaa
      721 gtcatcatcg gtgaggccga cttgttttgc aatggccgta atcggttcca atttcgtctg
      781 ctgtgcaatt tcgatatcgc tcaatacctt tgccatagta gttgtcactc ctgttctttt
      841 ctttgcataa aaaaagccgc tccgctggaa ggttgcccag acggtcggct tcaagaaatc
      901 atgctccccg tggtaactcc acttccgtca gtcacatgtt tcagatttat nnnnnnnnnn
      961 gattatggct tcattataca aaaaaagaac cctctggtca agggttcttt ttcaggacat
     1021 cttactggat cacgctggta tcgaggtgtt caaagcccgt gagcaggcct ttcacctgat
     1081 tggcgatctg ctgacagccg cctttttccc tgctttcgat gttcagcggc atgttcgggt
     1141 cgtggaggga catgcgcagc agggcccagc cgttagggaa tacgagacgc acgccttcat
     1201 aggacggctt ggcgatggcg atgcccgctt gagcggcccg ttcttcaaac tgtttaagta
     1261 cgtcagcgcc gtaggcctgg acgtcgtctg tgccggtgat tttcaggcgg aattcgacgc
     1321 cttccgcagg atggcccaat ttggcaatga ggtcgccgag ctgtttgcct tccttcttgg
     1381 ctttcgtcgc agcgatgagc agtttgacgg ccaggtacgc gccgtcgtcg aggtagtagt
     1441 tttcactcag cgcgccgtgc ccggatgttt cgatggccag cggcgaaacg gtgccggcct
     1501 tgttgaggcg gatacattcg tcgatgacat tcttatagcc ccgcatatag cggtgatgga
     1561 ccagatggag ttcatcttgg aggaacgtcg tcaattcgtc ggatgtgacc gagtcggtga
     1621 cgatagtgct gccgggataa tcaggcgcca agatggcggc catcatggcg atgagggcgt
     1681 tgcggttgat ttcttcccca tcgggcagga cggcgctcat gcggtccacg tcggtgtcga
     1741 aaatgaggcc caagtcggct ttgttgtcga ggacggcctt gcggatagag gccatggcat
     1801 cggcattttc cgggttgggg atatggttgg ggaacatgcc atccggttcg aggaactggc
     1861 tgcccgtcgt atcggcgccg agttccgcca ggaccttagt ggcaaagaaa ccgccgctgc
     1921 cgttgccggc gtcgatgacg atgtgcatcc cttccagagg acggtcgtac atgccggaac
     1981 agaggccggc acagatctta cggcgcaggt gcagggcgta gaggtcgatc agatccagcg
     2041 aggcaatggc tgccgatgat acggccatgg ccgacaggcc cgaagccagg gtcaggactt
     2101 ctttgacttc gctcttttcc aggccgccgt cgacggtaaa gaatttcagg ccattgcggt
     2161 tatagggcaa atggctggcc gtaatcatga tggagccatc catgtgggta tcttcaaaaa
     2221 taatggacat gaacatggcc ggcgtcgaag ccaggccgca gtccgtgacg tggacgccct
     2281 gaccgaggag gccgccgaat acggcttctt tcatcatgcc cgccgacacg cgggaatcgt
     2341 gtccgacggc aatgcgcagg tcactcgtct gtttattcag cttcttggct aaaaacttgg
     2401 caaatccagc ggcaacgcgg ttgaccgctt ccgggaccaa ggtgacgtct tcaccgggaa
     2461 cgccggcgat ggcgacgccg cggacgtcac tgccattctg taatttcatc atcgcttctg
     2521 tagttatcat gtagggatgt ttcctttctt cgatttagcg gacagcgtcc gctgcctctt
     2581 atcatactga aaatcaggcc aaaaggcaag cgtcgacaat ggcttctgcc gtcaggccgt
     2641 actctttcaa gaggtctccg gccttgccgg attcaccgaa acaatcctgg atgccgacgt
     2701 aagcctggcg cgtcggacag cggcgcgaca aggtttctgc cacggcgacg ctgtgttctt
     2761 cggcagtgac gacgaagccc gtcttcctgg ctgcagcgac gaccaggtct tcgtcgagcg
     2821 gtttgatggc ggccatgtcg atgaccatga cctgacagcc ccgattggcc aggatatcgg
     2881 ctgctttcag ggcttcctgg accatgaggc ccgtggcgat gacggcagca tcacagccgt
     2941 cgcgcaggac tgtccccttc ccccactgga agggacagcc agattgatag acgtcgtcga
     3001 ctttggcccg gcccatgcgc acgtagaccg gcccgtcatg agcgacagcc gcccggatga
     3061 cagcttccgt ttcggcgccg tcagccggac agacgacggt catattcggc agactgcgca
     3121 tgatggccat gtcttcaacg gactgatggg tcgccccgtc ttcgccgaca ctgaggccgg
     3181 cgtgggtcgc acagatcttg acgttgagct ttggcaggca gatggagttg cgaatctgtt
     3241 caaaggcccg acccgtgccg aagacggcaa aagtgctggc caggggaatc ttgccgcaag
     3301 cggcgaagcc cgccgccgtg cccatcatat cctgttccga aatgcccata tcgaaatagc
     3361 gttccggtgc taccttttta aaagcaatcg accgcgtcga cgaacccagg tcggcgtcga
     3421 ggacgacaat cctcggattt ttatacaatt ccgtttctaa gagatgcccg taggcatcgc
     3481 gcgttgccct tgccatcagc cttcgccccc taattcacgt aatgcccgtt cacattcttc
     3541 tttcgtcggc gccttgccat gccaggacac ggcattttcc atgaacgata cgcctttgcc
     3601 cttgacggtc tgggcgatga tgaccgtcgg cttccctttt gcggcctgcg cctggtgcag
     3661 tgcagcgccg atggcgtcgt aatcgtggcc gtcgatttcc aggacgttcc agcggaaggc
     3721 ccggaatttg tcggcaatgg gcatgacgtc catgacgtct tcattggacc cgtcgatctg
     3781 cagtccgtta tggtcgatga acagggtcag gttgtccagt ttatactgag ctgccgacat
     3841 agccgcttcc cagatctggc cttcttcgat ttcgccgtcg ccggtgatga cgtagacccg
     3901 ccagtccgcc ccgtccagct tggccgtcat ggccatgccg ttggcagcag acaagccctg
     3961 tcccagggat cccgtcgtca tgtcgatgcc cggcgtcgta ttcatgtcgg gattgccctg
     4021 gagcatggcg ccgcactggc gcaggtgcca caattcatct tccgggaaaa agccccgttc
     4081 ggccagcgtc gcatacagag ccggtacggt atggcccttg gagaggacga aacggtcccg
     4141 gtccggccag tgaggccgct tgggatcgat ccgcatgaca tcgaaataca agacttgcag
     4201 catatcgacg atggacaaag agccaccggg atgaccggac tgggccaggg tgaccatctt
     4261 gacgatatcc tgccgcacgt gagccgcatg acggctcaat tctttgctat ccatgagaaa
     4321 gccccctttt gccttccatt atagcacaaa agaggttgcc cggcggcagc ctcttttgtg
     4381 agtttgaagt ttgcagtttg tagtggatgg tcataaactt gaaaccatct tctacaaact
     4441 ccgtactaca aactataaag agaaatgggc ctgcatgatg gcaaaaagct tcgtgcaaca
     4501 gtctctttta agtggctagt ggttagtgga tggctttaga tgtaaatgcc cattggctct
     4561 cttcatagga gagctgtcag cacagctgac tgagaggttc aacctctccg gccctgcggg
     4621 ccacctctcc tatcaggaga ggcttacggt ctgcgacctg cggccgcggc cttcgcagtt
     4681 cagtttctta cgagccacga gccacgctca caacacatac ttctcgatgg cttcggcgac
     4741 ggcgccttcg ttattggtgg cgtcggtgac gacgtcgcag tattggcgga tgacggggtt
     4801 gaggttatgg acgccgacgg aaaggccggc ggccttcagc atgtcgatgt cgttgatgtt
     4861 gtcgccaatg gccatggttt cttcatgggc tacaccgagg atatccgcca atttccacag
     4921 gcccgcccct ttattgacgc ctttgtgcat gaattcgata tagcggttgc tggagaagct
     4981 gaccgtaatg tcgtccagca aggggcggat ttcagcgtgt atctgctgta aatagtcgta
     5041 atccgtattg gtcacgatga gcttgcagac ttcttctttg ccgcggatga aatcgaggtc
     5101 ctccgcttcg acaggcacgt agcccatgcg gccgtggagg aaggcttctt cgtcgggcgt
     5161 aatattcgtg atgtagacga catcacgcgt atagatatgc atacattgac cataggtaca
     5221 gcagcggcgg aaaatccggt cggccaggtc aaaggggatc gggtcccaga agagggagcg
     5281 gctgcccttg ttttccgtaa tattggcgcc gttgaagccg ataacatact ggtccgcctg
     5341 gtcgtagagg cccagagtcc tcagaacgcc ttcgatggaa cggaagcccc ggcccgtaca
     5401 gggcacgaat ttcacgcctt tggcaatggc tgccgtaatg gcttcgatat tgcgccggca
     5461 aatcgtcgcg tcgctgctga gcagcgtttc atccatatca caagcaatga tcttatactt
     5521 catgtcgtct ccttttcttt cgtaacggct ggggcatagt ggtcgtaaat cttccaggct
     5581 gccagtgctg ccagacattc aaagatccag aaggcgtgcc acacgccgtc cggtccataa
     5641 acgcggcaga ggagccacgc cgccggcagg ctgatgacga cgtagcgcaa cagggtgatg
     5701 gcaaaggacg gattgccctt ccccagccct tccagggcgc cagacgccgt aatagacaag
     5761 gccgacggta cgaatcccca ggcaatgatg cccagggccg acgcaccggc tgcgatggtt
     5821 tcctcacggg acgtgaacca gcccatgagg acgtccggta cggccaggca gatgaccagg
     5881 cctgccgcca tgaccaggcc gttgaggaac agcgtcgtcc gataaatgcg gcgcacgcgg
     5941 cccagttccc cagctccgta attatagccg atcagcggcc gcacaccttg aatgatacca
     6001 tttgtcggca tagaaagaaa ggtctggagc ttataataga cgccgaggat gaaaatatac
     6061 gtcggcgaaa aaggcgccag gagatggttc agggcgacga ggaagaccga cgacaggccc
     6121 atgttgaggg ccgccgggat gccgatgcca tagagccgtt tatcgagttc gatgtcccag
     6181 tggagatggc agcggcaaac ttcgacccgc agcggccgcc aatggcagac cacggcataa
     6241 aagaggaggt tcaccgtctg gcccaggccc gtcgcccagg ccgcgccttc aatccccatg
     6301 gccggaatcg gtcccaggcc aaagatgaga atcgggtcgc cgatgatatt gaccaggcag
     6361 cccagggaca agccggccat ggtgacctgc atgcggccga cggcctggaa catcttttca
     6421 tagaccaggt cgacgctgag gatcgtagaa aaggcaaaga cgatgacggc atagcgcagg
     6481 cccaggtcga tgagtcccgc gtcctgcgta aaggcccgca aaaaagacgg cacggcccag
     6541 atacagcctg ccgtcatgac cagaccatga atgacagcca ggaagacacc gtgtgtcgca
     6601 gccacgtcgg ccgtcccctt ctggccggcg ccgaggcaga tggaaataat ggcattgagg
     6661 ccgacgccaa agccgatgca gacggcattg acgaagttct gcatgggaaa gaccagggac
     6721 acagccgtca tggcctgttc actgatcatg gcgacgaaca ggctgtcgac gatattatag
     6781 agcgaattga cgatcatcga cacgacggcc ggcagggcca tagaagcgat gagcggcaaa
     6841 atcgcccgtt ctttcataaa cgattcgtcc atacgtcacc ttctgcggcg gtcgcgcagc
     6901 cagcgccaga gggcagcagc gcctttgatg accaggtaga agatgaagat attcactaac
     6961 aggccgagga tatcggcacc ccagcccagg gaaccccagc cgaagagatg gctgagcata
     7021 ctgcccagga acatgccgcc ggcaaagagg ccgataccgc gcatgacgct gcccaggcgg
     7081 gacgaagacg tcgtccggct ggcattggcc tgagccgttt cagccttgcg ggcctgcgtc
     7141 tgcccattcg gtttcgtatt gcccaggctg ggcgcttcat ttttcgtcgt ccccgttttc
     7201 ggtgtcacat tcttaggcgc cggggccggc agcgatttcg gcgccgatac gcgggcaccg
     7261 ccaccacctt tggcggcctg tacggaagac gggacagccg gcagggccag ggccgtccac
     7321 aaagcaaaga cacaaacggc gataagagtc gtttttttca taaataggct cctttataac
     7381 aaattgtgcg acggatccga ccctttcaaa acggccggtc ccccatgggg aaacatgatg
     7441 tcatatagat tcaatgtcga tagataatcg gccagcttgc cctgtacccc ggccagggct
     7501 tcgtggacgt cacagaaacc atagcggtga tggtacgggc actggaaatc gccgtccagg
     7561 cagctggtga tcatgttttc ccgttcgttt tcccgttcca tgacgttaat gatgtccatg
     7621 agggtaatgc cgtgcaaatc ccgtgtcagg cggcatccgc cctgggcgcc gcggatgatt
     7681 tcgatgaacc cggcccgttc gagtttcttg gaaatcttgt aggcgaactg ccgggggacg
     7741 agctgttcgt cgctgatttc ccgtatattt ttcagctgta aatcagccag ggatcgtaaa
     7801 atgcggatcg catagtctgt ttctctggta ataatcacga tgtaagacgc tccttctcat
     7861 tctgtataga ttaagtctat tataacgtga aatgacgaga aatgccagtc gtaaatgcct
     7921 tgcggttacc agaaaaaccc gatataataa atagtgtttg catgttttag ctttaactac
     7981 agatgaggtg tactatgtca ttaatcgatg aagtaaataa tcgccgtact tttgccatca
     8041 tctcccaccc tgatgccggc aagacgacgt tgactgaaaa attactgctc tacggaggtg
     8101 ccatccacct ggccggttcc gtcaaggccc gcaagacggc gaaacatgcg gtttctgact
     8161 ggatggaaat tgaaaaacag cgtggtatct ccgtcaccag ttcggtcctc cagttcgact
     8221 acgaaggcca tcatatcaat atcctcgaca cgccgggcca tcaggacttc agtgaagata
     8281 cgtaccggac cctcatggct gccgacagcg ccgtcatgct catcgacgtc gccaaaggcg
     8341 tcgaagccca gacgaagaag ctcttccgcg tctgtaaaga acggggcatc cccattttca
     8401 ccttcgtcaa caagatcgac cacttcggcc gcaacccctt cgacctcatg gatgaaatcg
     8461 aagacatcct gggcatccac gcctacccca tgaactggcc tatcggcctg aatggcaact
     8521 accagggcat ctacgaccgg gcttcgtcgt ctatcgaact ctttgaaaaa gacacgaccc
     8581 acggccagcg caaactggca tcgaccaagg gctcggcaga cgacccgatt ttcaaagaac
     8641 tcctcgacga agacgtccac cagggcctca tcgacgacat cgagctcctc gatgaagcgg
     8701 gcgacgaatt cgacatggaa aaagtcaagc gcggcgaact gacgccgatg ttcttcggct
     8761 cggctatgac caacttcggc gtcaagccct tcctggaaga attcctgcgc atggctccga
     8821 agccgcagcc gcgcaaatgc ctggaaggcg tcgtcgaacc gacatcggac cagttcacgt
     8881 cgttcgtctt caagatccag gccaacatga acccccagca tcacgaccgc atcgtcttca
     8941 tgcgcatctg ctcgggcaag tttgaaaaag gcatgactgt cacgcaccgc cagtccggca
     9001 agaccctgcg cctcatgcag ccccagcagt ttctggcaac ggaacggacc atcgtcgaag
     9061 atgcctatcc gggagacatc atcggcgtct tcgataacgg caccatgggc gtcggcgaca
     9121 ccctctattc gggcaagaag aaagtcacct tcaaggactt cccgaccttc ccgccggaac
     9181 tcttcgcccg catccgcgcc aaggattcga tgaagcgcaa acagttcctc aagggcatga
     9241 cccagctggc ccaggaaggg gccgtccaga tttatgaaaa cctgggctcc atggaaagct
     9301 acatcgtcgg tgc
//
LOCUS       DAWABZ010000067         9312 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2013_length_9312_cov_4.012423, whole genome shotgun sequence.
ACCESSION   DAWABZ010000067 DAWABZ010000000
VERSION     DAWABZ010000067.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 9312)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9312)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9312
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2013_length_9312_cov_4.012423"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2023 a         2522 c         2373 g         2394 t
ORIGIN      
        1 gcacaatctt attcaatcaa ttatttcgtt tctttgtgta atgtttcttt tttgcagaac
       61 gggcagtatt ttttcagttc caaacggtcc ggattgttct ttttgttttt gttcgtccgg
      121 tagttacgct gtttgcattc tgtgcatgcc aaagtgatag ctgtacgcat tccttacacc
      181 tctcttaaaa atagtcacaa gcctagtgtt acgtaagact gcctttataa tgtatcacaa
      241 cagtcccctc taagtcaagg aaatttttct gtaacttaaa aaagccaggc gttggtgaac
      301 gcctaacttc gattattcta acatgtgacg accgtcctgt caagagattt tccataattc
      361 catagcaggc cgcccagcca cagctgtccg ttctggcaac cgtcctgcgc cttccgtagg
      421 tcggcacctg acgggccaga aaggcgcggg acggccccta cgcacaaggc cgctggccgt
      481 gcattttttt tgtgacttgt ggcctgcgtc tatcgcggcg catccgcatc tgaatcgtac
      541 aacgccgcca gggcgtcctg gtatgttccc aagatgcgcc ccacctgtgc ttctatggcg
      601 tcgtggctgc cgtggcggtc gcggagcatg gccagccata tgtcttcatc gacgaagagg
      661 tattgttcat aagccgcggc gatgagttcc ttggcggagc ccatggcagc catgccctgg
      721 ccgtcccgct tcagggcttc gcgaaaggcc atcaagccca aatcaaaggc ttcctggaag
      781 atgtggacga tgccatggca gacgaagggg ttcgtcaaat ccgcttcccg agcctgctgc
      841 aaaagctgta tatgttcttg gaaattatcc tgccaatcca tagctgcctc cccaaacatc
      901 aaatatcaaa catcaaacct attataatgg atttccggca aaaaaagaag cagccctgct
      961 gtcaggagga gacagggctg cttcacgata aatggcttgg gaatttagcc agagattttc
     1021 tgttgtgcca gataggtttc ttccaggacc tgtttgcctg caatacccgg ctgggtcatt
     1081 tcctgcggcg acaggatgat gtccatctgt tctttcgtca gatagccgtg ttcgaggatg
     1141 atttcccgga tcgggcggcc tgtggcatag gcttccttgg ccagcatgga cgaccgttca
     1201 tagccgatgt gcggcaagag ggccgtgacg atgccgacgc tgtggtcgac ccaggactgg
     1261 caggcttttt tgttggcctt gacgcctttg agcaggtatt tgacgaaggc gtggatagcg
     1321 ttggtcaaat actgcatgga gtggaagagg ttgaaggcca tgaccggttc catgacgttc
     1381 aattcgaact ggccgttttc gacgcccagg ctgatggcca tgtcattgcc gatgatctgg
     1441 tagcacgtct ggtcgaggac ttcagccatg accggattga ctttgcccgg catgatggaa
     1501 gaaccgggct gacgggccgg cagcttcaat tcgttgaggc cgcagcgtgg accggaagcc
     1561 atgaggcgga agtcgttggc catcttgatg agcatgagag ccgtagcttt catattgccc
     1621 gatacttcgg caaagatatc cgtgttattg gtcatatcga tgaggttgcg ggccgtgacg
     1681 aagatatcac cggtcagcat ggaaagtttc gtggcgacgg ctttgatgta agccggttcg
     1741 gcgttgaggc ccgtgccgac ggccgtaccg cccatgttga tgacgtggac gccttcgagg
     1801 gaccgggaaa tgcgtttgcg gccgcgggcg acagccgaag cataggcgcc catttcctgg
     1861 ccgagcgtaa tgggaacggc atcctgcaag tgggtacggc ccatcttgat gacatctttg
     1921 aattcatcgg ctttgacatc cagttccgtt tccagttcct tcagtacttc tgtcagcttc
     1981 ttgcctttca tgctcaggca gactttgatg ctcgtcggga agacgtcgtt cgtcgactga
     2041 gccatgttgg cgtgattgtt cggcgagatg atgtcgtaac ggcctttttc tttgcctatg
     2101 atttctaagg cccggttgca caatacttcg ttcatattca tgtttacgga agtaccggca
     2161 ccgccctgga tcggatcaac agggaactgg tccagccact ggccgtcgat gatttcactg
     2221 gcagcctgta ccagggcatt gccgattttc ttgtcgagcc gtcccgtttc catgttggcg
     2281 agagcagctg cttttttgac aatggccatg gcctggatga agtccggatc cagatgttca
     2341 cctgtaatat ggaagttttc catagcccgc atggtctgta cgccataata aacattgtcc
     2401 ggtacattca tttcacctaa aaagtcatgt tctgttctca taatatcctc cccaataccc
     2461 atcttgcaat agatgcatta aatattttgt atacagtatt caagtaataa ttatagcaat
     2521 actgtagtaa ttaaattcat acccctccgg cgtccccacg ccgttacata cacaaaaggc
     2581 gctgttgaaa ttgctttcaa cagcgccttt gccgtacgta ctatgaattt acctatatca
     2641 tagtgcataa atgggcagat gtcaatattt attccgccac tttatgtttt atttagactt
     2701 cttgcagcct taggcttctt cgatggcccc agccggacag gagttcatgg cttcctgagc
     2761 agcgtcgttg ttagcttccg tcgattcagc ataggcttca gccatacccg tatcatcgtt
     2821 catacggaac acttccgggc aagtcccagc acacatacca caggcaatac acgtatcgtt
     2881 tactttatat ttcataatga gtcactcctt tgtccggacc gtctatccgg aaaacttagt
     2941 ccagtaagtc attccttact gtgcctatag tataccctgt tttcggccaa agggtcgttg
     3001 tatttacaac gctttagcaa attagcgata cgatgcctga taaatagcaa cgcagtcttc
     3061 ttcactcatg ggcatgcggt cggccatgaa taatcccgcc atggccgtct tggcattatg
     3121 ggccatttta tggaattcat cgggactgat gccgtagtcg gacatcttga ggttggcgac
     3181 gccgcatttt tcctgcaatt ctttcaaggc gtcgaggaag tcttccggtt tggccgcatc
     3241 cggacgcccc atatggcggg ccaggatgat gaaacggtcg tcacagacgt ggatgtcgat
     3301 catgtgcgag aaataagcca ggctgatcat aatcaggccg gcgccgtggg gcaaggcctg
     3361 gtgataggcg ctgagggcat gttccagcga gtgttcactc atgttggagc tgaggcacat
     3421 ttccaagccg ccgagatagt tgccgaaagc cacgcgttcc cgggccttca tgtcgatgcc
     3481 attctggacg gctgtcggca agtaggcgct aatctgggag atggcttcca aggcgaccat
     3541 ttcgctcatg agattggcct tattggccag atagccttcg atattgtgga acaaggcatc
     3601 gaaaccctgg taggccgtga acttcggtgg taccgtcgcc atgagttccg ggtcgatgat
     3661 ggccaggacc gggaagaggg atggtccttt gacgggcgtc ttttcattcg tgtcttcatt
     3721 ggtgatgacg ccgccggcat cggtttccga gccggtcccg gccgtcgtcg taatggcgac
     3781 gataggcagg ggcttgatgg caatgggttt gcccttgccc gtgccctggg tgatgtagtc
     3841 ccacaaatcg ccgtcattgg tggcgacgac ggcaatcccc ttggctgcat cgatgcagct
     3901 gccgccgcct aaggcgacga tgaagtcaca ttgattttct ctggccatgg cgccgccgcg
     3961 catgacagtc gatttcaagg gattcgcttc gacttggtcg aagaggaccg tttcgacgcc
     4021 ggctgtatgc agttcctttt ccgtccggtc caaatagcca ttgactttcg tcgatttccc
     4081 gttggaaatg accagcaggg ctttcttgcc gggaagggcc tgttcatgca aatgattgag
     4141 ctgaccggca ccaaataacg ctttcgtagg gacaaacatc tgaaaagaca cgataacacc
     4201 actcctatac tgggaataaa aaagagtctt atccacttgc gggtaagact ccatcgtcat
     4261 gaataaattt aatttacaag ccgattatac tataatattt tatttttatc aacttactat
     4321 tttatatttt aatttttctt tggctgcaag gaaatttccg ttacgccata gccagcattg
     4381 acaacggttt ttaacaacgt gtcatcgctc acggaatcag cgacgacgac ttctgcctgg
     4441 ccgcgcatca agtcaacttt gaccttgccc ttaaagccgt cgacggcttt aagggcttcg
     4501 gtcacgtgct tagagcagtt ctggcagacc atgccggtaa tgtagatata ttttttcgtc
     4561 ccctgaatat catcgtgatg gaagagattg ctgaaaaatc ccataagtca cgccccctct
     4621 gtttcatttt attatgaatt ctcatcatca atattatagc gtatcttgcc gatattgcaa
     4681 gattggtgat aaaggatact gtatacacaa atctatattt ataaaacata ttgacgatac
     4741 ttactgtatc atagtacact gtatttaaca aaaagtttat acaatttaac tttttcattg
     4801 tttttattaa aaaaagggag tgtgttttat gtcgtttttc catacgaaat ccgttgccca
     4861 gcttcagagc gatgcagaaa actcaaagct gagcaaaaac ctcagcgtct ttgacctgac
     4921 attcctcggc gtcggtggta tcatcggggc cggtgtcttc gtcctgaccg ggattgccgc
     4981 cgcccgctat gccggcccgg gcgtagcctt atcctttgtc attgccggta ttctctgtat
     5041 cctcgtcggc ctgacgtaca gtgaattttc ctccttcatc ccggcttcgg gcagtgtcta
     5101 ttcctacacc tttgcctccc tcggcgaagg catggccttc ctctgcggct ggtccctgct
     5161 cctggcctat acggtcacag ccagtgccgt cgctgtcggt ttctcaggct acctgtcggg
     5221 gatgacggcc tctttagggc tggtaatccc ggattatctc ctcaaaacgc cggctgaagg
     5281 cggcctggtc aatctgccgt ctatcattat tatcctttta ttgtgcttag tcctcatccg
     5341 cggtaccaaa cagagtgcga aactcaattt tgcccttatc ctcgtcgctt tagcggctat
     5401 catcgccttc gtcgtcatcg cagcgcctca cgcccaggca gctaatttat cgcccttcac
     5461 gcccttcggc gtccgcggcg tctgcagcgg ggcggctatc gtcttcttct cgtacatggg
     5521 cttcgacgcc gtcgcaacgt cagccgaaga atgtaagtgc ccgtcacgtg acctgccaat
     5581 cggcatcatc tcctccatcg tcatctgtat gaccttgtac attgccgtat ccctgtcgct
     5641 gacagctgcc atcaattaca cccagctcga tacaccggaa ccggttgcct atgccctgcg
     5701 tctcctcggc tggcccggca tggctaacct catcgccgtc ggcgtcctgg ccggtatcat
     5761 cacgacgctc atcgtctatc tcttcggtca gtcccgcgtc ttctttgccg tatcccgcga
     5821 cggcatgctg ccgcagaagc tctgcaagat tcatccgacc ttccacacac cgtatttgat
     5881 caccatttgg ggttccatcc tcatcgccgt catttccggc ttcgtcccgc tcatgcacat
     5941 cgtcgaattg tccaatacag ggaccttggc cgtcttcctg gtcaacttca tcggcatcgt
     6001 cgtcatgcgc cgcaaacacc cggaaatccc gcggaaattc cattgccccc tgctctacct
     6061 catcgctccc gtcggcatca tcgtctgctt ctacctcatc tatgaactgt cgacactgac
     6121 ccatatcctg ttcgcaggct ggattctctt aggcctcatc gtctactacg cctacagccg
     6181 taaacacagc attgtaagag aataaaggga agtgaaaaag gagctgtcgc atcgcggcag
     6241 ctcctttttg agtggctagt ggctagttgt tagtggctgg ttttaaattt aaagctatta
     6301 catcaaacat cataccattt ccatcgcctg tatgcgatat cgtaggggac gtcctgataa
     6361 ggcgtcccgt tgtgggaatc ccgtaaaatg ctgtttggag aaaacgccat tcgcaggccc
     6421 gcagaccgca gttcgcaaaa ccatgcctca ttaacccgtt acgtatatca tcgactgttc
     6481 gctacgggcc gccttacccg gccggcccct acgcaggccc cggtcggggc attttttacg
     6541 agctgagggg tactcgaagc gatgttatca gtttgagatt gtcgccgcac aaccgcatcc
     6601 gtaggagcaa gcccagcggg aggcccgcta cgcgctgagg gtttcccgaa gcgtcgttgt
     6661 cagtttgaat ctgtcgcggc gcagccgcat ctgaatccga gcgaaccgcg gcctgcgaac
     6721 tgcatctcat ctatatttta atggtggaag tccgtgcgat gtgttgcttc tacgtggtca
     6781 gcaaaggatg tttctttttc ctgtttggca aagcccttca acatccaggc catgttgcgg
     6841 ccgatgttgc gcatggtctg catcccttcc aaatctttgc gcacttcgtc cggcgtcatg
     6901 ccatggacca tgttccaata tgtcgaggta acgatgggca tgctggcaat gccgaaatat
     6961 ttctgaatga catcgacagc ggccgtggta ccgccgcggc gggccgatgc aatcgctgcg
     7021 ccaggtttac cggcaaagac cgggtagagc tcgcctacag ccgacgaata gaagacgcga
     7081 ttcaggaaag acaggatctg tccactcggg tgggcaaaat agaccggcgt cccgaagacg
     7141 aagccatcag cttccttcgc cttttccaag aagcggttga cggtatcatc attgtcatcc
     7201 ttaaagatac agcccttacc ctggcattta ttacagccga tgcagtcgcg gatcggccgg
     7261 ggccctaact ggaaaatttc ataatcgata ccacatcctt ttgaaagaaa tggaagccga
     7321 cggcattctc tatagaaaag aatatcccca ggtaccgcca aaggtcgaat atggactaac
     7381 ggaactgggg aaatctttga tgcctattct acatgaagtt tgtacgtggg gacaggatca
     7441 ctacgttcag cgctgaattc cgaaagcctg gaatccggga tagatgccct ggccgcgcaa
     7501 ttcgtcttca atctgcaaga gacggttgta tttcgctacg cgttcgctgc ggttcggcgc
     7561 accgctcttg atctgagccg tattcaaggc gacggccagg tcggcaatgg atgtatcttc
     7621 tgtttcaccg gaacggtgag aaacgatggc ccgatagccg gcacggtgcg ccatcttgat
     7681 ggcttccagc gtttccgata cggagccaat ctgattgagc ttgatgagga tggcattgcc
     7741 gcagccctgg gcaatccctt tggccaaacg cttcgtgttg gttacgaata aatcgtcgcc
     7801 cacgagctgt acgcgctggc ccaaggcttt cgtcaaggcc gtccagccat cccagtcttc
     7861 ttcatccaag ccgtcttcga tggaataaat gggataagcc tgggacaatt tctgccagtg
     7921 ggcgatgagt tcgtccgtcg taaaggactg gccactcttg ggcagaacgt aaacgcccgg
     7981 tttgggcccc ttccattcgc tggcagcggc atcgagggcc aggacgaaat ccttgcccgg
     8041 ttcatagccg gctttttcaa tggcctgaag gatatattgg atagcttctt cgtcgctggc
     8101 caaatcagga gcaaagccgc cttcgtcacc gacggaagta gccagcccct tggctttcaa
     8161 gagaccggcc agggcatgat agacttcgga gcaccagcgc aggccttccc ggaaggaagg
     8221 agcgcctgcc ggcatgatca tgaattcctg gacatcgacg gtattggcag catgagcccc
     8281 gccgttgagg atattcatca tcgggacggg cagataatta ccattgacgc cgccgaggaa
     8341 acgatagagg ggaatcccca gggacgcagc tgccgcacgg gcgcaggcca gcgatacggc
     8401 taagatggca ttggcaccga gccgcgattt atcttccgtc ccatcggcac gaatcatcag
     8461 gttgtcgata ccgtaagtat caaaaggatt ctgccctctc aagacatcct gtaaaactgt
     8521 attgacatga ttgacggcct ggctaacgcc tttgccgcca aagcgctgag catcgccatc
     8581 gcgcaattcc agggcttcaa actgccccgt cgaagcgccg ctgggagccg cgctgcggcc
     8641 caaagtccca tcggataaca acacatcggc ctgtaccgta ggattacccc gcgaatcaag
     8701 gatttcccgg ccaacgactt gttcaatacg gatatctttc atagtaaaac ctcccctgtc
     8761 gaaagtaata tatagcgaat accataattt cagtatcttt acagctatat tatacaatat
     8821 tagtctcctt tcgcataatg ctttatgaga atcgctgtta ttataattga gtatgttcgc
     8881 aggccgccgg tcgcagttag cggttagcgg tcagcagtta gccgttagca acggccgtcc
     8941 cacgagccac gaaccactag ccactatttt cccacctctc ctgccgaatc gaggcggtcc
     9001 accggaccgc tttcgtaggc gacgttctga aagccttttt ggcccgtcag gtgccgacca
     9061 gcaggaggcg caggacggct gccaaaacgg acagctgtgg ctaggcgtcc cgttgaggga
     9121 atcccgcgaa atgctgttcg gagaaaacgc cattcgcagg ccgcaggccg ccggtcgcag
     9181 gtcgcagtca gcaggcggtc caccggaccg ctttcgtagg ggacgtcctg tcaaggcgtc
     9241 ccgctacgcg ctgagggttt cccgaagcgt cgttgtcagt ttgagtctgt cgcggcggaa
     9301 cgccgcatct ga
//
LOCUS       DAWABZ010000068         9093 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2067_length_9093_cov_3.286457, whole genome shotgun sequence.
ACCESSION   DAWABZ010000068 DAWABZ010000000
VERSION     DAWABZ010000068.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 9093)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9093)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9093
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2067_length_9093_cov_3.286457"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7173..7182
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2284 a         2414 c         2247 g         2138 t
ORIGIN      
        1 ataaaagtta ttgattgatt tttgattcga tgagcagaag ctcaacaggg aggaaatatg
       61 gctaaataca tctttgtcac aggcggcgtc gtttcttcgt tagggaaagg cattacagca
      121 gcatcgctgg gccgtttgct gaagaaccgc ggctacaagg tcacgatcca gaagtttgac
      181 ccctatatca atatcgaccc gggcacgatg agcccgtacc agcacggcga agtcttcgtc
      241 accgacgatg gcacggaaac ggacctcgac ctcggtcact atgaacgctt catcgacatt
      301 aacctcggca agaactccaa cgtcacgacc ggcaagatct actggtcggt cctgcagaaa
      361 gaacggcgcg gtgactattt gggccatact gtccaggtca tcccgcacat caccaatgaa
      421 atcaagaacc gcgtctaccg cgtcggcgac gatgacaatg cggacatcgt catcaccgaa
      481 atcggcggta ctgtcggcga catcgaaagc ctgccgttcc tggaagctat ccgccagatc
      541 aagaaggaag tcggccgcaa tgatgtcttg tacatccacg tcacactcgt accgtatatc
      601 ggcgctgctg gcgaactgaa gaccaaaccg acccagcaca gcgtcaagga actgcgcagc
      661 atcggcatcc agccggacat catcgtctgc cggacggaac gccccttgtc ggatgaaatg
      721 aaagaaaagc tggccctgtt ctgcgacatc gacaaagatg ccgtcatcga aaacaagacc
      781 ttgaacagca tctatgaagt acccctcctc ttgtcggaag aagggatgga ccgcatcgtc
      841 ctggaaaaac tcgacctgcc caatcgtccc tgcgatatgg aagactggaa gaaaatggtc
      901 catgccattt acaatacgga aaagaccctg gaaatcgcct tggtcggtaa atacgtcgcc
      961 ctccacgacg cctacctcag cgtcgctgaa gctctgaacc atgctggcat cgcctataag
     1021 acgaaagtca atatccactg gatcgattcg gaaaccctgg aagacccgaa cctctcgatg
     1081 gaagaagtct tcaaaggcat cgacggcatt gtcgttcccg gcggcttcgg caaccgcggc
     1141 gtcgaaggca agatccgtgc cgtccagtat gcccgtgaaa acaacgtgcc ttacctcggc
     1201 ctctgcctgg gtatgcagtg cgctgtcatg gaattcgccc gccacgtcgt cggcctcgaa
     1261 ggcgctacgt cgtcggaatt ccatgaagat gccaaattcc cggtcatcga cctcatgccc
     1321 gaccaggtcg acatctccga taaaggcggc accatgcgcc tcggtctcta tccctgcaag
     1381 ctgaccgaag gtacgaaggc ccatgaagtc tatggcacca acctcattta cgaacgccat
     1441 cgccatcgct gggaattcaa caataaatac cgcacggaaa tggtcaacgc cggcctcgtc
     1501 ctctcgggca catcgccgga cgaccgcctg gtagaaatcg tcgaactcaa ggatcacccc
     1561 tggtttgtag gctgccagtt ccatccggaa ttcaaatccc gcccgaccaa agcccatccg
     1621 ttattcaaag gcctgatcaa aacggcttta gaattaaaag gggaataatt agtgggggcc
     1681 gccgcatgaa gggttctgcc atcatgccag gcccctttta atttatagtt tgtagtacgg
     1741 agtttgtaga agatggcttt aaatttaaaa aacttcacta aaaactacaa actgcaaact
     1801 taatactccc aaaggcgctg tcataagaca gcgccttttt cgcgtctatg cggcagacgc
     1861 atataggatg catatatatg tgatttattg aacgtatggc aagttgttct agagccgtta
     1921 aaatacgata aaagttagtt acggcgacat gaaaaataaa ctattttttg ttattatgca
     1981 tccttataaa atagggtaag aacggccttt atactataca aaacaggtga cgtatacgga
     2041 aatgtaaact acgttatctc tttgtatccg gacaattttt gacttctttt tcctgcgccc
     2101 ggcaggtcat aaaaataagt taaatgtgta actttgctct gattttcaca ttgaataata
     2161 tgctacataa tgatataatg taagcgattg aaagcatgat tcatgttttt attatttttc
     2221 attatataat aatgaggtga tttttgatgt tgaatacctt taaaagaggt ggggtccatc
     2281 ctgacgatgg caagatctat gcaaaagaca aagccataga aaccccggca gtaccggctc
     2341 aagtcgtcat tcccatgagc cagcactttg gtgcgccttg tacgccgacc gtcaaagtcg
     2401 gtgacctggt caagaaaggc cagcttatcg ggacgagtga cgcattcttg catgccgaca
     2461 tccatgcatc tacgtccggc aaagtcatca aagttgcgcc tatgccgcac aacatgatgg
     2521 tcaaatgcat ggctgtcgtc atcgaagcag atggcaaaga tgaatgggca gaagggattc
     2581 cccaggctca cgactggaaa cagctcgaca gcaaggaaat cctggaactc atcaagaaag
     2641 ccggcgtcgt cggctgcggc ggtgctactt tcccggctca tgtcaaattg gctccgaaca
     2701 aaccggtcga tacgttcatc gtcaacggtg ccgaatgtga accgtatctg acttgtgact
     2761 atcgtgccat ggtcgaaaag gaaacgacgg acaaactcgt cacgggcgtc cagatctgca
     2821 tgaaagtcct cggcgtcaag aaggctttca tcggcattga agacaacaaa ccggaagcca
     2881 tccagaacct gaccgaagct ttcaaagata ttcctgaagt tacggttctc gcttgtaaga
     2941 cgaagtaccc ccagggtgct gaaaagatga tgatcgaagc ctgcacaggt cgtcaggtcg
     3001 aaccgggcgg acttcctatg aacgtcggct gcgtcgtcag caacgtcggt acggtcattg
     3061 ccatcaccga tgctgtctgc cagggcatcc ccttcatcga acgccttacg accgttaccg
     3121 gtgactgcat caaagaaccg aagaacctcc gtctccgtat cggtacgact ttccaggaag
     3181 ccatcgacta ctgcggcggc ttctccaaac agccggatcg cctgatcgcc ggcggcccga
     3241 tgatgggtct ggcccagtat cagctcgatg tgccgatcac caaaggggct tccggtattc
     3301 tggctctctc tccggaaaaa tgccaggtcg gcgaagaatc tccgtgcatc cgctgcggcc
     3361 gttgcgtaga agcatgtccc atgggcctca ttccgagcca gctcagcatc ttctcgtcct
     3421 gccaggcatg ggataaatgc atggaatacg gcgtcatgaa ctgtgttgaa tgtggcagct
     3481 gtgtctatac ctgcccggca aaacgtaaca ttgtccagta tatccgcaat gcgaaaggcc
     3541 agtgcaaagc tatccagcgc gtagcgcagg ctaaagctga agcacagaaa gccaagaact
     3601 aattggaaga agggatatca tgagtccaga agctaaagga caaacaaatg gaaaggatgc
     3661 taaagacatg caggaattta aacttaccgt agcatcttca ccgcatgttc gctgtaacga
     3721 aaccattcct aagatcatgt ggaatgtcgt cgccgccctt gtaccggctg ctgcgttcgg
     3781 cgtctactat ttcggtgtca atgctctcgt caatatcatc gtcgcaattg tctcggcagt
     3841 tgtatttgaa ttcttatggg aaaaactgat gcacaaacgc gtcaccatca aagatggttc
     3901 tgccgtcatc acgggccttc tcctggctat gtgctgcccg ccgagccttc cctggtggat
     3961 gagcatcatc ggttccttcc tggcaatcgt cgtttgtaaa caggccatgg gcggccttgg
     4021 ctacaacatc ttcaacccgg ctcacgtcgg ccgtgctggt ttgatggttt cctggccgat
     4081 cgccatgacg acctggaccc agctcaatgg gaccgtcatc gacggtgtcg ctggggctac
     4141 accgctgaac gtcttcaaac acggcggcgt agatgctctc ttcaatctct tcggtactaa
     4201 cgattggggc agcatttatc agagcttatt cttcgggttc cgcaacggtt ccctcggtga
     4261 aacgtcgaca gtcctcctca tcctcggtgg tctgtggctg atctacaaag gctacatcaa
     4321 ctggcaggtt ccggtcatca tgatcgctac cgtcggcgtc ctcatgggtg ctttctatgg
     4381 cgtcaacgtt gcaatctttg aaatgatggc tggcggcctc atcatcggcg ccttcttcat
     4441 ggcaaccgat atggtcacgg ctccgattac cctgaaaggc cagatgatct tcgcattggg
     4501 ctgcggcctg attacggtcc tcatccgtac tctcggcggt tatccggaag gggtttgcta
     4561 ctccatcttg ctcatgaata ccttgactcc gctcatcgac cgtctggtca aaccgaccgt
     4621 ctttggcgca gtaaaaaagg gggctaaata aaatggcaga caaaaacgaa agcggaatct
     4681 tcaaggttgc gatgaacctt atcctgacct gcctggtttc cggctgtatc atcggcgtcg
     4741 tctatgctgc taccagcaag attgcctatg aaaaagctga acagatgaaa caggattcca
     4801 tgaaatccct cgttccgcag gctgaaaaat tcgatcccgt ccctggcgaa aaagatacct
     4861 tcgtcgctga acagggcggc aagaaagtgg cttatatcat tccgacggct ccgaaaggtt
     4921 acggcggcgc tatcaagatg ctgacggctg tttccatgga tggcacggtt atggactaca
     4981 cggtcctcga agccaacgaa actccgggcc tcggcgacag aggtgctaaa tctccgttta
     5041 aagatcagtt caaaggcaaa aaactggaaa acttggaagt caccaagagt gctagcgaaa
     5101 aggataaaat ccaggctatg accggtgcta ccatttcttc ccgtgctttc actttgggcg
     5161 ttaaacaggc tgttcagaaa gcagcagagt tagggggtaa ataataatga atctttggaa
     5221 agtatttact agaggcatta ttgaagaaaa cccgtacttc gtcctggcct tgagcctgtg
     5281 cccgggcctt gccgttacga caagcatcgt caatggcctg accatgggct gcacagtatg
     5341 gttcgtcatc acggctaaca atacggtcgt ttccatcatt cacaagctga tcaacaaaaa
     5401 agtccgtatc cctgtttaca tcacctgtat cgctactatc gtaacgtgcg tacagctgtt
     5461 cttacaggct tttgctccgg atctgtacaa agccctgggc gtatacctcg acctggtcgt
     5521 cgtatttgct atcatcctgg ctcgtgctga atcggttgca tcgaaaaacg gcgtcgtcgt
     5581 ttccttcctc gacggcatgg gcatgggcat tggcttcacc attgctatgt tcctcatttc
     5641 cttcgtccgt gaaatcctcg gcaacggtac cttctttggt atccctgtct tcggtccgtc
     5701 ctatcagccg gctctgatca tggccttgcc gcctggagcc ttcatgctca tcggtttcat
     5761 catggctggt ttcaaaatct ggaatgaaca tgttgaaaga cagagacaac tgaaagggag
     5821 tgaaggataa tggaatactt tacgctgttc atcggggctg tcttgattaa caacttcgta
     5881 ttgaccaagt tcctcggttt gtgtatcttc ttcggcgttt ccaagaacct gaacgcctct
     5941 gtcggcatgg gcttcgccgt cactgccgtc atcactatga gtactatcct ggcatggtgc
     6001 ctgtacaact tagtcctggt accgcttcat ttggaattcc tgcgcacgat ttccttcgtt
     6061 atcttgatcg caagtttcgt acagttactg gaaatcgtca tcaagaaaga atcgccgacc
     6121 ttgtataaca tgtggggcat ttacctcatg ctgatcgcta cgaactgtat cgtcctgtcc
     6181 gtcccggtca tcagtgtcac cagcggttat aacttcttgg aaaacatcgt cttcgctgtt
     6241 ggcgcaggcc tcggcttcgc cgtagctctg atcctcatgg caagctgccg tgaaaaattg
     6301 gactatgccg ctgttcccca cgctctgaaa ggcacaccaa tcgctttcat cgtcgccggt
     6361 atgatgtccc tggccttcct cggcttctcc ggtatgattt aaggcaaagg agtgaattag
     6421 attatgtacg attatacaac gatagctatg tatgctgtcg gcgtcatggt ttgcatcggt
     6481 atcgtcttcg gctttatcct tgcaagtgct gataagaaat ttgccatgga agaaaatccg
     6541 ctcatcggcg aagtaaaaga agcacttcct cagggccagt gcggtgcctg cggtttcgcc
     6601 ggctgtgcaa aatatgcgga agccgtcgtc ctcgacccga acgttccgcc taacctctgt
     6661 gttcctggta aagctgaagt cgctgctgtc gtcgctaagt tgacaggcaa agtcgctgaa
     6721 gctacggaac cgaaatacgc tcacatcaaa tgccgcggcg gcaatggcat tgccaaactg
     6781 gcttatgaat acaaaggtgt tcatgactgc gcggctgcta agatcgtcca gcagggcccg
     6841 aaattctgta aattcggctg tctcggcttc ggcaactgca cgcgtgcctg cccgttcggt
     6901 gctatgagca tgggcgaaaa tggcctgcct gtcgtcaaca agacgctctg cacaggctgc
     6961 ggtaaatgcg cttctgtctg cccgaaccac gtcatcgaac tcctgccgat cagtgccaaa
     7021 gtcagcatca gctgcagctc caaagatttc ggcgctccgg ctatgaagaa ctgcaaggcc
     7081 ctgtgcctcg gctgcggttc ctgcatgcgt aactgctcgc atcaggaaga aggggctatc
     7141 aagatcatcg acaatctggc cgtcgtcaac cannnnnnnn nnggacatct gccatacctg
     7201 tgacaatgct acctgcctga ccaagtgccc gactggtgcc atcaacgaaa tgatcaaagg
     7261 ctctttcaag aaagaagaaa acgtaggtta atacatggat aagaaacggt atgtatgggt
     7321 tcttctcacc atggcgttcc tcatcatcct tagttgctcc ggatgcagtc tgcatccgga
     7381 gtcttctttt gataggagcg gtgtcgccat ggatacgacg gtctccctgc gggcgtccgg
     7441 gaaagaagcc aaagaagcag tcgatgaagg ctttgaacgc ctgaatcagc tggacaaact
     7501 ggcaggacag aatgaagaca gcgacgtaag ccgtatcaat gccgctgccg gcaaagcata
     7561 cgtccaggtc gacccggcca tttatgaaat gattgaatac gccaaggatt attcggccaa
     7621 gagccagggc gaatgggaca ttaccgttgg cgtcatgacc aatttatggc gcatcggcaa
     7681 cgacgaccag cacgtgccgt cagccgatga gatttcccag gccctgatga aggtcaacta
     7741 taaggacatc ctcctgcggc cggaagacca cagcgtcatg ctggctaaac cgggcatggc
     7801 catcgacctg ggcggcgtgg ccaaaggcta tgctgtcgat gaaatccgca agatttatga
     7861 aaaacaccac atcgaaagcg gcctcatcaa cctcggcgcc agctcgatgt atgccgtcgg
     7921 caagacgacc aagggcaagc cctggcgcat tggtatcaaa catccccgct ctgacgacga
     7981 caagagcttc ctgggcatcg tatccatcga gaaccaggcc ctgtccacat caggcgacta
     8041 tgaacgctat ttcatccagg atggcgtccg ctaccaccat atcttcgacc cccgcacggg
     8101 ctatcccgcc cggtccggcg tcatgagcga ctctgtcgtc atcgacggtt ccgtacctca
     8161 tgcaggcatg ctcagcgaca tgatgacgac tatcgtcttc gtcctgggac cgcagaaggg
     8221 gatggaactg gcaagcagca tggaaggcgt caactgtgaa atcacaggca gtgacaatgc
     8281 cgtctacatg acccagggct ttgccgataa tttctctgat ttgaatagtg atttccatct
     8341 ttccgattcg gaataaacca gcctccatag aagtaaaaag tatagcagaa aaaaaattaa
     8401 tatctttaag tctatttact ttttcattcg tttgtaagat aatagatatg taaattttag
     8461 aaattatgga gggtagtatc tacatatgga tcatatcgat gacaaaattt taacgatact
     8521 caaacacaat ggcaaagctt cggctacgga aatcagcaag gtcgtcggcc tgtccattcc
     8581 ggctgttgcc gaacgcatcc gcaagatgga acacaacggc atcatcgaag gctacggtgc
     8641 caaaatcagc cgccgcaaga ccggttacaa cgtgacggct ttcatcatgg tcaatctcga
     8701 acgctctggc gatgtcgaag aattccgctc gaaaatcgtc cagtacccgg aagtcctgga
     8761 atgtcaccac gtcgtcggcg cctttgacta cttgctcaaa gtgctggtca agacaccgga
     8821 tgacctggaa cacttcctca tgcaggtatt gaacgaaatc ccgacggtcg cctcgtccca
     8881 gaccttcatg tgcctgtcga cactgaagga agaatggaat gtgtaaagag cgtcgtgcct
     8941 cgtagttcgt agggggtgcc ccaagggcgc cccgcagaca ttgacgaacc gctaacaact
     9001 aaccagaacg ggccgccctt tgggacggcc cctacgataa aaggggctgt aacaaaatga
     9061 gtttttttgc tcattttgtt gcagccttcc ttc
//
LOCUS       DAWABZ010000069         9072 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2073_length_9072_cov_2.769214, whole genome shotgun sequence.
ACCESSION   DAWABZ010000069 DAWABZ010000000
VERSION     DAWABZ010000069.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9072)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9072
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2073_length_9072_cov_2.769214"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5765..5783
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2083 a         2501 c         2685 g         1784 t
ORIGIN      
        1 gtttttgcat acataaaaag agcctgtgca cgattacgtg cgacaggccc ttttattatt
       61 tatactttct ttaccttgca ggctacggtg tagatgacgg cacacatgac taagtaggcg
      121 acgatgccga ggtaggtggc tcccggcgtg acgacccagt ctttgaggaa ccagtcgatg
      181 ccgccgatgt gcaggacggc gctgatgagg ctgccgatgg cgccgccggc gacgaagccc
      241 gaagcgacgg tgctgccttc actgaggcgc agggcgttga gggcatcgtc tttgctgctg
      301 tgggagacga agtacgatac gaggccgccg atgaggacgg gcgtgttgat ttccatggga
      361 agatacgcgc cgagggcaaa ggccaggggc gggaccttca tcatccacag gaggaaggcg
      421 aagaaggcgc cggccatgta cagcggccag ggagcggcac cgccgtccat catgggtttg
      481 acgatggctg ccatggcgtt ggcctgcggc gctgccaggg cgcccggtcc cgtgtagccg
      541 taggcgtggt cgaggaggaa gatgacgccg acagcgatga agctggagac gatgatggac
      601 aggatctgcc actgctgcat cttcttcggc gttgccccgg tcaggtaggc gactttcagt
      661 tccgacatga agttgccggc tacggccagg gtcgagcaga ggaaggcggc catgagcagg
      721 atggcgatga taccggagct gccgctcatg ccggcattgc ccatgaagac ggccgagacg
      781 atgatcatga agatggtcat gcccgaaacg ggttcgttcc ccgtaaaggc gatggagctg
      841 atgccgacga cggagaggag gaacgagaag atgaggatga ttgccccggc cagcagggtc
      901 tgggtgatgt tctgcgagca ggtgacgtgg aagaagagcg tgaacagggc gcacatggcc
      961 agggttcccg tgacaatctg gctcatgggc aggtcctgct gggtccgcag gaggccggcc
     1021 gacgaagccg ggcccgacgt gaacaggtcc gaagcggccc ggcggatgac ccggccgacg
     1081 acgccggaca tggagaccag gccgataatg ccggccatgg ccagcatgcc gatgccgatg
     1141 tgacggacgt actgcgagaa aacggcgtcg atcggcgcat cggccaggag gacggcctgg
     1201 ccgtggaggg tcatgacgtg ttcgccgccg aggtagtaga cgacggggat gcagacgaac
     1261 cacgagaaga acgagcccgc tgcgatgata gccgcgtagc gcaggcccgt gaagtagccg
     1321 atgcccagga gggcggcgtc gttatccagg ctgaaagcca gcttgtattt cgccgccgcg
     1381 gcctggcccc aggagaaggt cagcgtcgac agtacttcct gccaccagcc cagggtgttg
     1441 aggacgaagt cataggccat ggccaggaga ccgctgaaga ccatgacatg agctttggag
     1501 ccttcgttac aggtcaggac ttcggcagcg gcccggccgg acgggaaggg gtaagccgca
     1561 tccatttctt cacagaagta gcggcggaac gtcgtcgcca ggaggacgcc caggacgccg
     1621 ccgaggacga tggggatgac catttccgta taggtcagct ggctgtcgag ctgcaagatg
     1681 aacagggccg gcaggatgaa catgacgccg ccgatgatgt tggtcccggc actggcgatg
     1741 gcctggatgt ggaccgtttc cgggaaggca ttcttgcgct tgaagaggac ggccatgccc
     1801 atggccagga cggagacggg cgcaaaggca tcgacggtct ggccgatttt caggcagaga
     1861 tagccgacgg ccagggagaa gagggcggcg aagaacagcc cccagccgac gacataagca
     1921 ttgatttcaa cgtaattctg gtccggagac atgggcggat cataagggcg ccgctccgga
     1981 tgggattcag acatgataaa aacctcctaa cctcctaaaa gtataaagat tagctggaaa
     2041 atcaaacgat taacctgttt aatactattt aattttaaaa gaatagccta gggctgtcaa
     2101 tgggaagata cgttagatat agattctgta aataaaaata attatccgtt gataaattgt
     2161 tgaatatgga gtataatagg tgacataaga agacgaaaaa aggaggctta cttatgaata
     2221 ttgtagtttt ggtaaagcag gttcctgcga tttctgatat cgaaatcgcc aaagacaaca
     2281 acttggtccg tgtcggcgct ccgtcgatgc tcaacccggt tgataaacac gccatcgaag
     2341 cggccgtagc cgtcaaggat gccatcggtg gtacggtgac gatcctgacc atgggcaacg
     2401 ccctggctgg tgaaatgatg cgtgatggca ttgctatcgg tgccgataaa ggtgtcctcg
     2461 tctcggacga acgcatggct ggctccgata cgctggcaac aggcctggta ttggccaagg
     2521 ccattgaaaa attgggcggt gccgacctgg tcttcacggg caagcgctcg acggatggcg
     2581 atacggggca gatcccgccg gctattgccc agcgtttggg tatggccctc atttcctatg
     2641 ccaattcggt cagtgtcgac ggcacgacgg taacggctac gcgcctaaac cacgacggca
     2701 ttgaaacggt acaggctcag ctcccggctg tcgtctcggt tacggaaaag agcaatacgc
     2761 cgcgttctcc gaagatccgc ggcaagatgg ctgccaagaa agccgtcttc gatgtctgga
     2821 aagtggaaga cctgggcctc gatgcagcaa gtgtcgggaa atctggttcg gcgacgaaag
     2881 tcacggaact cttcgcaccg gaaccgaatc cggtcggcgt catgatcgaa ggcgctacgc
     2941 cggcagacgc tgcggctaat ctcgtcaaag aactgactgc caagaacttg ctgtaaggag
     3001 ggctgaatta tggaattgaa acagaatatc tgcgtatata tagaaatcgc cggcggtgcc
     3061 ctgagcccgc tgagcatcga attgttgggt aaagcccgtc agctggccga tgccaagagc
     3121 aaacaggtcg tcgccttcgt cgccggtgcc gatacggcca aagccagcca ggaagccatt
     3181 tcctatggcg ctgatgccgt cgtcgctgtc gaagacagtg ccctggctgc ctacgattcc
     3241 gtcttgtaca ccaaagctat cgccgctttg gctgaaaaat atgaacccag cgtcatcctc
     3301 atcggcggtt cgcccgatgg ccgtgacctc ggcggccgcc tgtcggctca gctcggcgtc
     3361 ggcctggtcg cagactgcac ggacgtccgc tttgaaggcg atgacgatac gctgacctgg
     3421 atccgtccgg cttatacggg caagctcttc gtcaagatcc agacgacgac gcgcccccag
     3481 ctggctacca tcagcgacaa gattttccac ggcacgccgg ctgacccggc ccggaaaggc
     3541 gaaatcatca aagaaagcgt cgacttcgcc ggtgctgctc cgtccgtcaa agtgacgaac
     3601 tttgaaccgg tcgaaaaaga agcggaagaa ctgaccatca cgacagccga tatcgtcgtc
     3661 ggtgccggcc gcggcgtcca gactgaagac ggcatgaaag ccgttcaggc ctttgctcag
     3721 cgcatcggcg cagccttcgg cgtatccaag cctctcgtcg acaaagaatg ggctccctat
     3781 gaatggcaga tcggcattac aggcaagaaa atcgccccga agatttacat cgccctgggc
     3841 atttccggcg ccatccagca caaactcggc atccaggacg ccggcctcat cattgccgtc
     3901 aacaaagacc cggatgcacc gatcttcaaa ttcgctcact acggcatcgt aggcgacctc
     3961 tttgaagtca tgccggaact ggaaaaacaa attaaaaaga tccgtggcta gaaaatagtt
     4021 tgtagtacgg agtttatagt ttttagtgaa aacctttaaa tttaaaacca tccgctaact
     4081 gctaaaaaag gtgctgtctt catgcgacag cgcctttttt atgttataat acccttatca
     4141 tatacgaaaa tgttgtgaaa aaatacggag gtggttccat ggattctttt gctgcccgcc
     4201 aggcctgccg cgacggccgc tatgacgagg tactggctat gtatggcaag gcggcaccgg
     4261 accggtcttg gacgtgttgg gattattatt actatgccta tgccctgcgc aagacgaagc
     4321 agtacaagaa ggggcgggaa atcgcccggg ccggcatgat cgccttcccc gatttcatca
     4381 atctccacgg gatttactgc tggtgcctct attatttata tatccagaaa ttcgatgaac
     4441 ggacgcaggc accggaggat ttccgccgcg ccgtcgacgc catcttgaaa tacagtcagc
     4501 aagggccgta tacgccgtat gccctggccg tgtggcgcat ggtcgatgtc ctcaagaacc
     4561 agcccggtca ggccggactg atggggtcct acttgtgccg cctcgacccg gaccagctgc
     4621 cggaccagga gaagaccgtg actttgaagg ggcgggaacg ggtcattgcc tcggaccggg
     4681 aacgctggta ttccctcatg agccgcatcc tggtcaagga agagaagtac gacgactgca
     4741 tcaccttgtg ccggcaggcc ctgaactatt tccctcagct ccatcatgac aacgacatct
     4801 ggttttctta ccgcatcgcc ctctgcctgc tgcgccaggg ccgcgtcggg gaagggcggc
     4861 agcgcctgga gaacctgctg aagtacaagc agcactggat tcttttccgg ggcctgttct
     4921 ttgccgccca ggccgagggc gacagcgcgg ccatgcgccg ctatggggcg tcggccttcc
     4981 tggctggcgg cgagttcaag gccaaggtca atttcatggt ccagttcgcc caggctttgg
     5041 agaatatggg cggttatgag aagatggcct atttccatta cctcctggcc aaacgggtcc
     5101 gcatggacca gcattggaag gtcaaggccg aactcctggc caagaccgac agctatgctt
     5161 ttcccgaacc ggaccggcag gagctgatgg acgggctcca tggtttctgg atggacgaga
     5221 agcacgccgg ccagacgcgc cacaagggga ccatcgaaaa aatcctgccc ggcagcaagg
     5281 ccggcttcct ccgggaatac ggcggcaatc agtattattt ccgtaccgct tcgctgtacc
     5341 gcgtccgccc ggaggaaggg gagaaggtca cgttctatgt agaggacttc tttgagacag
     5401 ggaaagaaaa accggctcat cgcgccgtgg acatcgaacc ggttttgcta tatgataaga
     5461 agtgaattta ctagagaaag tgagtggcac tcatgatttt atcaggcaag gaaattgtca
     5521 agcatttagg taaggaaatc atcattgaac ctttccatcc ggaacgggtc aatcccaata
     5581 gctataacct gtctttgtac aatgaactga tggtctatga ccaccaagaa ctggatatgg
     5641 ccaagcccaa tccggcatcg accatttata tccccaagtc gggctacgtc ttgcagccta
     5701 acaagctcta tctgggccgg accaatgaat atacccggac tgacggctat atccccatgc
     5761 tcgannnnnn nnnnnnnnnn nnngttttgc cggttactgg acgctggaaa tcttctgtgt
     5821 ccagcctatc cgcatctatc cgaacgtgga aatctgccag atctactatc acgatatcga
     5881 cggcgaatac gatacgtatc agcacggcaa gtaccagaac aatacgggta tccagcccag
     5941 tatgctctgg aaagactttg aaaagatgaa ataaagggaa gtgtcttcat gaaagaaata
     6001 gatatgttag ccatggcccg gacgaacggt ttccgcctgg gcaatgccca ggaccgccag
     6061 ggcggcacgg gctgcacggt cctcgtgttc ccggaaagtg cgccctgtgc tgtcgacgtc
     6121 cgcggcggcg gcccggccag ccgggaatcg gaactgctca gtcctatggc ggctgcgtcg
     6181 ggcatccacg ccatcctgct cagcggcggc agtgcctttg gcctcaatgc ctcgactggc
     6241 gtcatgcagt atttggaaga acagcacatc ggcttcgata cgcgcatctg caaggtgccc
     6301 ctcgtcgtcg aatcgtgcat ctatgatctg ggctgcggcg acaacgtccg ccctgacgcc
     6361 gccatgggct atgcggcctg ccaggcgtcg gaaagtgggg acttccgcca aggctgttac
     6421 ggtgccggta cgggggcgac ggtcggcaag ctctgcggcc cggactatat gatgaaatcc
     6481 ggcctcggcg cctatgccgt ccaggtcggc gaccttcagg tcggtgccgt cgtcgccgtc
     6541 aatgccgtcg gcgatgtcct cgacgaaaac ggccagattc tggccggtat gcgcagccgc
     6601 gatggccagg gttttgccga tacgcggcgg gtcatgctcg aagaagccgg ccagacgccg
     6661 accctctttt cccagcgcgc cgtcgggacg acgaccaata cgactatcgg tgccgtcgtc
     6721 accaatgggc agttcgacaa ggcccagctg aaaaaaatcg ccgccctcgg cagcaacggc
     6781 cttgtccgga ccatccgtcc cgtcaatacg acggcggacg gcgattctct ctatgccgcc
     6841 agtgtcggca gcgtttcggc cgaattgagc ctgaccggga ccatcgccgc ctacgtcgtc
     6901 gaacaggcca tccgccgcgc cgtccgccag gccgaaagta tgtacggcgt gccggcgtac
     6961 cagtaaaagg gtagcccctt acgccaggcc gggtttgtcc tgccggttgt agtatagagt
     7021 ttgtagtttg tagtgggggt ataactgcga actgcgacct gcggcctgcg aaccagggat
     7081 tcccgcaggc cattaataac gaaatgagga actaaattat gtacgattac gtcatcgtcg
     7141 gcagcggcct ctttggctgt gtctttgccc acgagatgca tcaggccggc caacagtgcc
     7201 tggtcctgga acgtcggggc catatcggcg gcaatgtcta ttgtgaagag aaggacggca
     7261 tccatatcca tcgctatggt gcccacattt tccatacgtc ggaccgccgg gtctgggact
     7321 atgtcaatca gttcgtcgaa ttcaatcatt ttatcaatgc gcccattgcc aattataaag
     7381 gcgaactgta caatctgcct ttcaatatga atacctttgc caagatgtgg ggcatcatca
     7441 cgccggaaca ggcggcggcc aagatcgaag gccagcgccg ggcggcaggg attacggagc
     7501 ctaagaatct ggaagaacag gccatttccc tcatcggcac ggatatttat acaaaattaa
     7561 tcaaaggcta tacggaaaaa cagtggggcc ggagctgtac ggacctgccg gcattcatca
     7621 tcaagcgcct gcccgtgcgc tatactttcg acaataatta tttcgacgac tgttggcagg
     7681 gcatccccga agggggctac aatgtcctca tcgacgccct tctggaaggc atcgaagtgc
     7741 ggactggcgt cgattataac cgcgaacggg cgtcgtacct ggatatagcc cgtaaagtcg
     7801 tctataccgg tcccattgac gagtatttcg gctatcgcct gggccatctg gcctatcggg
     7861 gcctgcattt tgaaacggaa cgttataacg aggtcaacca tcaaggtgtg gccgtcatga
     7921 actataccga ccgggaaacg ccgtacacgc ggacgattga acacaagcat tttgaatttg
     7981 gcaggcagcc tgtgacgtat gtcaccaagg aatacccggc tgaatggcag gccggcgaag
     8041 aagcctatta tcctgtcaac gatgcggtga accagaattt ataccataaa tatgcggcct
     8101 tagcccgcca tgaacgcaat gttatattcg gcggccgact ggcagaatat acgtattacg
     8161 atatggacga tgtcatcgcc ggcgccctgg ctgctgtaga aaaggaagga taccatgcag
     8221 aaacgaattc gtaaggccgt catcccggcg gcaggctatg ggacgcgctt tttgccggtc
     8281 accaaggcca tccctaagga aatgattccc attgtcgaca aaccggtcat ccagtacatc
     8341 gtcgaagaag ccctgcagag cggcatcgaa gaaatcctca tcatcacggg ccacggcaag
     8401 cgggccattg aagaccattt cgataccaat atcgacctgg agctgcagct gcgccagcag
     8461 gggaaggacc agctgctgca tctggtccag gacatttcgt ccatcaatat ccattatatc
     8521 cgccagaagc acatgcgcgg cctgggcgat gccatccgct gtgccgaatc gttcatcgat
     8581 aacgagccct ttgccgtcct cttgggcgac gacgtcgtct acaatcctga gcagccggcg
     8641 ctgaaacaga tgatggatgc cttcagccgc ctgggggcta ctatgctcgg ctgtcaggaa
     8701 gtgccgcagg aactggtatc gcgctacggc atcgtccagg gccagccgac ggacgacgac
     8761 cgcgtcgtca agctcatcga catggtcgaa aagccggctg tcgacgaagc gccgagccgc
     8821 ctggccgcgc tgggccgcta tatcctgacg ccggatatct ttgaaatcct gcgccgcgtc
     8881 cagcccggca agggcgggga aatccagctc accgacgccc tgcgcctcat ggccgaccgg
     8941 gaagcggtct acgcctacac cttcagcggc cgcagctacg acacgggcaa caaactgggc
     9001 tttttgaaag ccaccgtcga atacgccctg cgccgggaag acttgggcga agccttccgg
     9061 gaatacctgc gc
//
LOCUS       DAWABZ010000070         9024 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2086_length_9024_cov_3.512209, whole genome shotgun sequence.
ACCESSION   DAWABZ010000070 DAWABZ010000000
VERSION     DAWABZ010000070.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 9024)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9024)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9024
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2086_length_9024_cov_3.512209"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2116 a         2358 c         2395 g         2155 t
ORIGIN      
        1 tagcgaatac gagtatattc tctcctatta tcgcattttt gttcatgtaa atgcaaggta
       61 aaatagaaaa caaattcaga aatatgacct tttatgtata ctatattttg cataaaaatt
      121 atagcctgtt ttaaggctat acgcaaagtg aaaatatgta aatatttagt tgaaataatg
      181 tgctaggtga tgtatgatga aatcacagaa aatcctctat gcaggtagcg aaagaagata
      241 ccagtgtaat tgatgaaagg agcaatgtta ttatgcaaga caagcaatac gtcatggccc
      301 ttgatgccgg caccacgagt aatcgcgcta ttattttcga caatgaatca catatcgtca
      361 gcgtcagcca gaaagaattc actcagtatt tcccgaaacc gggctgggta gaacatgacg
      421 ccaatgaaat tttccacagc atggtcgaag tcatgcgcga agccctggaa caggccaata
      481 tgacggctag tgaaatctcg gctatcggta ttactaacca gcgtgaaacg acggtcgtct
      541 gggacaagaa cacaggcgac cccgtgtaca acgccatcgt atggcagtcc cgccagaccg
      601 cgccgatttg tgaagacctc aaagcccgcg gcctggtcga tgaattccgt gaaaagacgg
      661 gcctggtcat cgatgcctac ttctcgggca cgaaggtcaa atggattttg gacaatgtcg
      721 aaggcgcccg ggaaaaagcc gaaaaaggcg atctcctctt cgggaccatc gactgctggc
      781 tggtctggaa actgacaggt ggccaagtcc acatcaccga ctattccaac gcatcccgta
      841 cacttatgtt caacatcaaa gaactgaagt gggatgacca gcttcttgaa tatttaaccg
      901 ttcctaaatg catgctcccg gaagtccgcc cgtccagtga agtctatggc tgcacggatc
      961 cggctatcct cggcggtccg gtcaacatcg ccggtatcgc cggtgaccag caggcggctc
     1021 tctttggtca gacctgctat gaaccgggta tggccaagaa cacctacggt acaggctgct
     1081 tcatgctcat gaacacaggc acgacgccga ttccttccaa gaacggcctg gtcacgacca
     1141 ttgcctgggg ccttgacggc aaagtcgaat atgccctgga aggttctatc ttcgtcgccg
     1201 gttcggctat ccagtggctc cgcgacggcc tgcgcctcat cgacacggct cctgattcgg
     1261 aatgggtcgc taaacgcgtt cccgactcgg acggcgtcta tgtcgtaccg gcattcgtcg
     1321 gtctcggcgc tccctactgg gatatggatg cccgcggctt gatcatcggc atcacgcgcg
     1381 gtactaagaa atctcacatc gttcgtgcaa ctctcgattc tatggcttac cagacgaaag
     1441 atgtcctcgg cgccatggaa gcagactctg gcatcaaact ggctgccctc aaagtcgacg
     1501 gcggagctgt tgccaacaac atgctcatgc agttccaggc cgatatcctc ggcgttcctg
     1561 tcgatcgtcc gcaggtcatc gaaacgacgg ctctcggcgc cgcttatctg gctggcctgg
     1621 ctgtcggcgt atgggattcc aaagaagaac tcgtatcgag ctggaaactc cagaatcgtt
     1681 tcgaaccgac catggatcct gaaaaagcag cagggtatta taaaggctgg cgcaaagctg
     1741 tcaaacatgc aatgcactgg cttagtgatg aagactaaga gagaggtgct ggaatcatga
     1801 tgtttgacaa cttattagga gaatttatcg ggacgatggt cctcatcgtc ttcggggccg
     1861 gcgtctgcgc ctgcaacacg ctgaccaaat cgaaaggcca gaacggcggc tggatttgta
     1921 taaccgcagg atggggtttc gccgtaactc tcggcgttta cacggcaacg accctcggtg
     1981 ctccgcaggg tgacctgaac ccggctgtta cgctcggcaa gacgatgatc gggatttaca
     2041 gtgttcctca gttcttcgcc acgtccatcg ctcagatcct gggcggtatc ctcggcgcag
     2101 ctatcgtctg gctggcttac ctgccccatt gggctgaaac ggaagataaa ccggcgaaac
     2161 tcggtatctt ctgcacggct ccggctatcc gcaactatcc ggccaacttc ctttgtgaat
     2221 tcattgctac tttcttcctg atgttcgtta tctggatgat tttctccaat aaagtcggtg
     2281 ccattcctcc tggagtcggc ccgtatatcg tcggcatcct catctgggct ttgggtatga
     2341 gccttggcgg tcctacgggg tacgccatga acccggctcg agatttgggt cctcgtattg
     2401 ctcatgctat cctgccgatt gccggtaaag gcggttcgga ctgggcttat gcctgggttc
     2461 ctgttttcgg cccgctggct ggtgcctttg ctgcctacgt cgtagccatg gctttacatg
     2521 tcttctaatt tatttaatat atggttttgg gaaagggctt gtcattcatc ggcaagccct
     2581 ctcctttact taagtttata aatttgttta taaattttga acataatttc atacttaatc
     2641 aaaggtaata ttcctatatt tttgcatgaa aaaatagtat aattcggcta tgtgaaaata
     2701 tgtaaaaata gtcttgcaat tattttcaca aaccgatata ataagggcat caaagatagt
     2761 gatacttggt atttacagag aaagaggttg tgaacatgac aaaacaagca gatgtcgtcg
     2821 taattggcgg cggtattacc ggcacagcca ttttgcacga actggcaaag tacaatcttc
     2881 gtgcagtcct ggttgaacag gaaccggaac tggctgccgg cacgacaaag gccaacagtg
     2941 ccatccttca cgcaggtttc gatgcgccta cgggcagtat gaaagcccgc atgaatgccg
     3001 caggcaatgc gatgtatcat gaattgaaag acgaattgga tctggacatc cgctggtccg
     3061 gctcgtatgt agccgccctc gacgacgaac agatggacgt cctcaaggaa ctcctcgaac
     3121 ggggcaatgc caatggcgtt cccggactga aaatcgtcag cggcgatgaa atgcgcaaag
     3181 aagaaccgaa tgtcagcaag gacatcaaag gcgcactctg ggctccgact gccggcatct
     3241 gctggccttt cggcctggcc ctggcttttg cggaaaatgc cgtcatcaac ggcgctgaag
     3301 tcatccgtga atgtcaggtt acgggcatta ccgtagaaga cggtgccgtc aaagccgtcg
     3361 aaacggacaa gggtactatc gaaacgaaat acgtcatcaa tgccgccggc gtccatgtcg
     3421 acgaaatcag ccgccttgcc ggggacgata cctttaagat ccatccgcgc cgcggcgaat
     3481 acatcctctt tgataaaacg gctcagaaag acctcgttta ctcgccgatt ttcccgacac
     3541 cgacgaaaat gggcaaaggc atcctggtct gcgcgacgac acatggcaac gtcttcgtcg
     3601 gcccgaacgc ccaggatatg ccggatgaag aaaaagacga cacggccgtc accattccgg
     3661 gcatggacga catcctggac aaagcccgcc gtctggtccc gaacatccct gtaggcgcta
     3721 ccattacgga atttgccggt gtccgcgccg tatccagcac gggcgacttc atcctcggcc
     3781 cgtcggaaaa gacgaaaggc ctcatccagg ctgccggcat ccagtctccg ggcctgactt
     3841 cggctccggc tatcgccaaa tatctcgtcg acggcatcgt cgctgaaacc ggtgcgacag
     3901 ccaagacgga ctataagaaa ggccgtccgg ctcagccctg cttccgcatg atggctgaag
     3961 ctgaccagaa agccctcatc gccaaggatc cccgttatgg ccgcgtcatc tgtcgctgcg
     4021 aaacgattac ggaaggggaa atcgtcgatg ccatccatcg ccccgtcggt gcccgcaccg
     4081 tcgacggtgt caaacgccgc acgcgtgccg gcatgggccg ctgccagggt ggcttctgcg
     4141 gacctcgtgt tacacagatt ttgtcccgtg aattgaacat ccccgttacg gaagtacgga
     4201 aagaaatgcg taattcttac atgttctaca acaaagaaag cggaggtaag gcataatggg
     4261 tgaacaactt tatgatatta tcatcgttgg cggcggcccg gcaggcttat ccgctgccta
     4321 cagcgcttgg cagaacggcg ctaaaaaaat tctcgtcctc gaacgtgacc gcgaagctgg
     4381 cggcatcctc cagcagtgca tccacaatgg tttcggcctc catcacttca aagaagaact
     4441 gaccggcccg ggctacgccc agcgctgcta tgacctggtc aaggataaac cggaaatcga
     4501 aatcctcgtc gacaccatgg tcctcagcgt caatctcgac aagacggtta cggctgtcag
     4561 cccggctcac ggcctgatga aagtacaggg caagacgatc atcctcacca tgggctgccg
     4621 cgaacgtaca cgcggtgcca tccgtatccc cggttatcgt ccggctggcg tctttacggc
     4681 cggtgcagcg cagcgcatgg tcaacatgga aggctatctg cccggtaaga aaatcgtcat
     4741 cctcggcagt ggcgacatcg gcctcatcat ggcccgccgc atgaccctgg aaggctgtaa
     4801 agtccaggct gtcctggaaa tctgcccctt ctcgaacggc ttgacccgta atatggtaca
     4861 atgtctgtat gactatgata ttcctttgta tctgtctcat accattacgg ccatccacgg
     4921 caaagaccgg gtcaacggca ttacggccgc catagtcgac gaccatatga aacccattcc
     4981 gggcacggaa ttcgacatcg actgcgatac gctgctcctg tccgtcggcc tgatcccgga
     5041 aaatgaattg tcccgcggct tggggctggc tatgcatccg ctcaccaatg gtcctatcgt
     5101 cgaccagcat cgtgaaacga gcatccctgg catctttgca gccggcaacg tcgtccacgt
     5161 ccatgacctg gtagacttcg tatcggaaga agctgaaatt gccggtaaat acgctgccct
     5221 caaggcacag gaacagctgc cggaagacgt agccgacgtc aaggtcgaag ccatcgacga
     5281 agtccgcacc tgcgttcccc agcacgtccg cgctacggca gaaggcgaaa cgatccgctt
     5341 gttcatgcgt gtccgcaaac ccatgaagaa agtccgcctc actgtcaaga gcggtgacga
     5401 tacgctgctg acgaaagtcc tccccgtcgc caaaccgagt gaaatgatcg ccgtcaacgt
     5461 accggctgaa aaagccaagg gccatcatgc ggattggacc gtttccgtaa aggaggaagc
     5521 ataacatgag tgtagaaacg aaacaactga actgcatcaa ctgcccgctg ggctgcctgc
     5581 ttactgttac cctggaagac ggtaagatta ccaaggtcac aggcaatacg tgcccgcgcg
     5641 gtgaaaaata tgcccatcag gaattgaccg acccgcagcg catgctgacc tcgacggtcc
     5701 gcatcgaagg cggcgaactg ccgctcctgc cggtcgtatc caagacgacg ttgccgaaag
     5761 gcaaaatcct cgactgtgca gcagccctgc gcagcgtcga agtcaaagcc ccggtccata
     5821 caggcgacgt catcgtcaaa gatatcctgg gcctcggcgt agatatcgtg gccagccgcg
     5881 atatgggcaa agaataagca tcggtaccaa tattccctct tacatatacg agaaaggggc
     5941 tgtcgcatgt gcgacagccc ctttctcgtt gctcgttgtt cgtggctagt ggctagcgaa
     6001 aagaggttgc aggggtcgtc ctctacagcg atcttcttca gcagccgtcc tgtcgttttt
     6061 tacagtcatg ccctgacggg ctgaaaaggc acgtgcttta gagggactca ttaatttgct
     6121 tgatttatta ggcgaacatg gtacaataaa aacagagata gtccgatggt ggtacatcgg
     6181 tctcctctgt ggtttatgtt tgaagaaggc cgtgtaccgc gagtacgcgg tttttttcat
     6241 gctaaaggtt tatcgcttcg ttgctgaaat aatcagcact ataagagttg cgaatgcaat
     6301 catcaatgac aatgcttcgt atacactcat gctgatcacc tcgctttcgc gagggaaacc
     6361 gaatacatcg aactatctca tggagttcag tataccagat ttactaccag gcttcaagga
     6421 aaagccattg ttcgtaattc ataaagccat accatcatta tcgaataggt tgtggtgaaa
     6481 tctcgcagcg ggccgcccat cctctgctgt ccgtttcagc agccatcttg cgccttccgt
     6541 tggtcggcac ccgatgggct ggaaaggctc tgggacggcc cctacgcagc taaccactaa
     6601 ccactaacca cttaaaagag gctgtcgcat gtgcgacagc ctcttttctc atgtattggt
     6661 tggaggatgg tctatttatt tcgttttctg ctggatgaag cccggcgtcg tgaggtaacg
     6721 gtcgccgtta tccggcagga aggctacgat ggtcttgccg gcgttttccg gacgttttgc
     6781 gacttccgaa gcggcataga gagctgcacc gccggagata ccgatgagga ggccttcgga
     6841 ttcggtgaat ttctgagccg tttcataagc ctgttctgtc gtaaccgtga agacttcgtc
     6901 gtagatcttg gtatccaatg tttccggaac gaagtttgcg ccgatgccct ggagtttgtg
     6961 cgggcctgca tggccttcgg agaggagcgg cgaatctttc ggttcgatag cgatgatctg
     7021 gacggacggt ttcttttctt tgaggtactg gccgacgccg gagagcgtgc cgcctgtgcc
     7081 gacaccagct aagaagatgt cgacagcgcc gtctgtatct tcatagattt ccgggcccgt
     7141 cgtcaggcga tggatttccg gattgaccgg gttggtgaac tggccgggga tgaaggaatc
     7201 ggggatttct ttggccaatt cgttggcttt gtcgacagcg cctttcatgc cggtcttgcc
     7261 cggtgtgagg acgatttcag cgccataagc ggaaatgagg ttgcggcgtt cgatagacat
     7321 ggtttccggc atggtgaaga tggcgcggta gcctttggca gcagctacgg cagccaggcc
     7381 gatacccgta ttgccactgg tcggttcgat gatgaccgag ccttttttga ggatgccttt
     7441 cttttcagcg tcttcgatca tggctttggc gatgcggtct ttggcggagc cggccgggtt
     7501 gaaagcgtcg agtttgacca aaaggttagc agctacgcca gcggcgttgt tgtaacgttc
     7561 cggtttcaat aatggtgtgt tgccgatcaa ctgtaacatg gatgtgtaga ttttgctcat
     7621 gagaatctct ccttttccta tgattcatcc cccgatgaat gcaaaaatat gttgaagtat
     7681 agtaaggtat accaaatggg tatagatttt atgtacacct gtagagggat ttttcatttc
     7741 catacagggt gacaacgaag tttattattt caatggccgc attttgccaa cgcagcgttc
     7801 atcttgctgg tggtccatca tttcgacgcg gtgctgcaag agggctacct gatgctgcag
     7861 ggcgatgatc tgatcttcga tggggtcggg caggcggttg tggttgaggt cgacgtcgcg
     7921 ttcgctgtgg ctggccttga ggcgcttgcc tttctggacg acgacgcggc ccgggatgcc
     7981 gacgactgtc gagtacggcg gcacttcttt gaggacgacg gaaccggcgc cgatttttga
     8041 gctttcgccg acggtgaagg acccgaggac cttggcgccg caggcgacga cgacgtagtc
     8101 gccgatggtc ggatggcgtt tgcccttttc cttacccgtc ccgccgaggg tgacaccctg
     8161 atagagggac acgttgttgc cgatgaccgt cgtttctccg atgacgatgc ccatgccatg
     8221 gtcgatgaac agaccttcgc cgatttgggc gccgggatgg atctcgatgc ccgtgaagaa
     8281 gcgggaaatc gtggaaatga ggcgtgccgt gacgaaccac tgatgcttat agaagaagtg
     8341 ggccaggcga tgggcccaga tggcatgaag gccggaatag cagagcaaga cttccagcgt
     8401 gttcttggcg gctggatcgc ggtccttgat gacgcgaatg tcgttgcgaa gtgatttgaa
     8461 catggcgtag cgccctcctg tgtcgtaaaa aagtaaataa aaaaagacct gccgtcatac
     8521 agagacggtg gtccgtggtt ccactctgat tgcagtcttt cgactgccac tcaatacccg
     8581 taacggcggc aaccgggatg acctactcac gttcaatcat cctgctccca aaggcattcc
     8641 atcacattca acttcgatag gggctttcag ccggtggccc ttactctctg tgccgttatc
     8701 ctgtgtgtac ttgttttggt cattgcattt ttgttttcgt gttgaacaca tgatacccct
     8761 tcacaggacg tttgtcaata gaaaaattgt aaaaagatga caaaattggt gtcttattca
     8821 aaaaggctga tagaatcacg ttctttatat atccatataa gggatttgcc gtttttcgtt
     8881 catcgttcgt cgtaaccagg gcttttgttt ttttcgacct acgacgaacg gcctgcgatg
     8941 aacgatgttt tcataaagac tcattatttt tgattaaatt tgtctgcata caatatccat
     9001 gtttagtaga tatgaattgt actc
//
LOCUS       DAWABZ010000071         9017 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2089_length_9017_cov_2.956260, whole genome shotgun sequence.
ACCESSION   DAWABZ010000071 DAWABZ010000000
VERSION     DAWABZ010000071.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 9017)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9017)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9017
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2089_length_9017_cov_2.956260"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1715..1724
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2024 a         2567 c         2455 g         1961 t
ORIGIN      
        1 aaaaaaagag agctgtcgca caaagccgaa atggcttaat gcgacagccc cttttaagtt
       61 gttagtggct agcagttagt ggttagcgga tagcttaaaa tttaaaagtt ttctctgcaa
      121 attataaagc aacagggaca gtcgcatgat ggtcaaaacc ttcgtgcgac tgtccctgtt
      181 tgtctgagct gcctatgcgg cagttaacca gggaagaatc cctgtttttc tttttcaaaa
      241 tttctaagct gcctacacgg cagttaacag gttactctta tttaaccaaa atttcaggca
      301 tttgtcaaaa ggcttgattt accaggcctg caggcaaaga aaaaatttta ggtcaaaaaa
      361 tcgtgagtct gtcgcggcgg aacgccgcat ctgaatccgc ggcggcctgc gaactgcgaa
      421 cggcggcctg cgtcctttaa tcatttatgt tccactttat gtgtcactgc gctcaaggcc
      481 gctgcgacgg gacccatacc gcgcaggacc atggggaaaa agaggaagag gacacccatg
      541 gcgccgacgt tggccgaaat gacgatgacc gccatcccgg gctggcagac ggtcccaaag
      601 agaagaccgt tcgggcaatt cgcctgaacg gtctgtccgg catcggggcc atcttccatg
      661 cgcaaggtga cgatctgatc gcccacacgg cttccgtctt cctgcagatt atcgcggaca
      721 atatcgacaa cgcgggcctt ttcaaagacc cggccatcgc tgttgaccag ctgcggctta
      781 tcgacctggt tatactgcca gaggataagg ccgccggcca ccatcagcac gacgatgacg
      841 accaggcgga accagcggga gtggatcatg ctcaattacg taaaaatgtc agtcctgttc
      901 atttcccttc tgccgccaat aggccgcttc gacggtatcc ttctccacgc cttccccctt
      961 ttccagggca cgggcataat tcttcatgcc ctctttatag ccagacgcag ccgactgggc
     1021 gaaccaatac gccgcctgac agaggtcatg gcggacgccg tgtccgtctc gatagaggac
     1081 gcccaggttg ttctgggccg gtccgtcgcc ggccatggcc gcttcataat accagtgcgc
     1141 cgccagttcc cagtcctggg ggacgccgcg gcccgtctca tagaggtatc ccaggttgtt
     1201 ctgggcaatg gtctggccct tcccggcggc ctgggcgtac caataggcag cctggtcgta
     1261 atcccgcttg ccggcatagc cgaactggta catgatggcc gtattgcact ggccgtcggc
     1321 atcgtcgtgc cgcgccgccc ggccgtacca gtgcagggcc tgggcgtagt cctggggcag
     1381 gccgcagccc gtttcataga gatggcccag attgttctcg gctgcgccgt agccttgatt
     1441 ggcggcccgg aggtaccagt acacggcctg ttccaagtca atctctatcc ccagaccgtg
     1501 ttcatactga taggccaagt tgtactgact ggcggcgtgg cccttctggg cggcccggtg
     1561 ataccaatag atagctttcc tatataaaac atctttatct tctatatccg catcgaaagc
     1621 ttcaaaaaaa tagtcatcgc ccatgtcaca ctgcgcttga gcatcgcctt caaaagcttt
     1681 ccgttctttt tcgtccatat atccgtctcc tttcnnnnnn nnnnacaaaa taagtgtagc
     1741 taaaggttcc ttcctttgtc aaatattttt ctatttttct ttttgtccta ttgacttttt
     1801 gacttttgac gatataataa tactagagct tgaggaaagc tcctaagaat gattacttcc
     1861 accataagta aggaggtctt aattatggga aacgaaaaat atacgcaaaa ggtcattgaa
     1921 gccttccagt ctgcccagca gattgcctcg ctccattata accaggaact ctcatcggtc
     1981 catatgctca tgggcttgac gaaagaaccg gaaggcctgc tcaatacgat tttctcggaa
     2041 tgtcacactg acgtccccat gctccaggcc cgcttggaac agttgttgaa gaaaatcccg
     2101 tctgtcaaag gctccagcca gttgtccatg tcgacagaaa tggtccgtgt catcggcaag
     2161 gctcagcagt tggccgactc catgcacgat gaatatatca gcacggaaca tatcctcatg
     2221 ggcatcgtca gcgaaagcga cgacgaagtc cagcagttgt gccgtgaatt tggcttgacc
     2281 caggacaaga tcatgagtac catcaaagcc aaccgcaagg ccaacgtcaa cagcgataac
     2341 ccggaagaaa actacaaggc cctggaaaaa tacggccgcg acttgaccgc tgctgcccgc
     2401 cagaataaat tggaccccgt catcggccgt gacgacgaaa tccgccgcac catcgaaatt
     2461 ctctcgcgcc gccgtaagaa caacccggtc ctcatcggtg aaccgggtgt cggcaagacg
     2521 gctatcgtcg aaggcctggc ccgccgtatc gtcagcggcg acgtcccgga atcgctgaag
     2581 aacaagaccc tctactccct cgatatgggt tccctcatcg ccggtgccaa ataccgtggc
     2641 gaatttgaag aacgactcaa atcggtcctc aatgaaatcg ccaaatcgga cggccagatt
     2701 ctcctcttca tcgatgaaat ccacactgtc gtcggtgccg gcgcctctga aggctccatg
     2761 gatgcaggca acatcctgaa accgatgctg gcccgcggtg acctgcgctg catcggcgct
     2821 acgacgctca atgaatacca gaaatacatc gaaaaagatg ccgctttgga acgtcgtttc
     2881 cagccggtca tggtcgatca gccttctgtt gaagacacga tcaccattct ccgtggtctg
     2941 aaagaacgct acgaagtcca tcatggcgtc cgcatccgtg ataaagccct ggtcgctgcc
     3001 gccgtcttgt cggaccgcta catttccgac cgtttcctgc ccgataaggc catcgacctc
     3061 gtcgacgaag ctgccgccaa actgcgtacg gaaatcgaat ccatgccgac gccgattgac
     3121 gaactgtgcc ataagatcat gcaactggaa atcgaagaac agtccctgaa caaagaaacg
     3181 gacgacgctt ccaaagaacg cctggctaag atcacggaaa cgaagaacga actgaaagaa
     3241 aaagaaagca agctcaaagc tcagtgggat aaagaaaaac agtccatcct gcgcacgcag
     3301 ggcctgaaga aagaaatcga cgccgtcaag agcgacatgg cccaggcaga acgcaattac
     3361 gacctggcca aggcttcgga attgaagtac ggcaaactgc cggaactgca gaaacagttg
     3421 aaggaacagg aagcctacct ggctgaacac caggattcgc agctcctcaa agaagaagtc
     3481 ggcgaagaag atatcgccca ggtcgtcagc cgctggacgg gtatcccggt caccaagatg
     3541 atgacgggcg aacgggaaaa acttctgcac ctcgacgaaa cgctgcataa acgggtcgtc
     3601 ggccaggacg atgccgtccg cgtcgtgagc gatgctatca tccgcgcccg tgccggtatc
     3661 aaggatccga accgtccgat tggttccttc atcttcctcg gcccgaccgg tgtcggcaag
     3721 accgaactgg ccaagaccct ggctgaagcc ctcttcgatg atgaacgcaa catcatccgt
     3781 atcgatatgt cggaatacat ggaaaaacat accgtatccc gcctgatcgg ggctcctccg
     3841 ggatatgtcg gctatgatga agggggccag ctcactgaag ccgtccgccg tcgtccgtac
     3901 agcgtcatcc tcctcgacga aatcgaaaag gcccatccgg acatcttcaa cgtcctcttg
     3961 cagattctcg atgatggccg cctgaccgat ggcaaaggcc gtgtagtcaa cttcaagaac
     4021 accatcatca tcatgaccag taacctcggc tcccacgaaa tcctcgaatc gaaggattac
     4081 gacgaagcca acaagaaagt ccgtgaactg cttaaacagt acttccgtcc ggaattcctc
     4141 aaccgtgtcg acgacatcgt cgtcttcaaa gcgctccaga aagaccaggt acgcaatatc
     4201 gccgctatcc tcctggaacg cctgggcaaa cgtctggaaa aacaggtcaa gatccacctc
     4261 acctggaccg acgaagccct gcaggaattg gccgacaaag gcttcgaccc gcagttcggt
     4321 gcccgtccgc tgcgccgcct gatcacgcat accgtcgaaa cggccctgag ccgcgacatc
     4381 atcgccggta ctatccgtga aggcgataca gtcaccatcg gctacgacgg caatggcttc
     4441 accttcacgc ccatgcgtgc ccatgccgcg tgagtgtggc gagctgttag ccgttagctg
     4501 ttagctgtta gtatgtggaa aacgctgtca gacactaacc cctagctgct aggccctaac
     4561 ccctggcagt tggcggtcag cggctagcag tcagctgtca gcagtgagtg gctggcggct
     4621 ggcgcataaa ggacccgttc ctgatgcaaa aaaacatcgg gaacgggtct ttttttctgt
     4681 ttacattagc ccgaaaacag tatataataa gctgtaacta agtaacgggt ggagatttat
     4741 gaataacaca ttaggaaaac atgtactcat cgatttttat aactgcaagg cttccttttc
     4801 ggaggccgaa gatttgcagc ccttggtaga gcgcgcgttc gacctcgtcg gcgcccctgt
     4861 cgaagggatt acattcttcc atatcgacga cgaactgacg tgtattgccg tttccggcaa
     4921 cgcacatatt tgtattcacg cctatcctcc cctgtcctac gtggcagtgg acatctacag
     4981 ttttaatatc gacctcaaga cgagccagat catgagcacc ctgaaggtaa acctccgctc
     5041 agaccgcatc aaagcgacaa gtatccgccg cggtgatttc ggctctatcc gagacatgcg
     5101 cccgaaacgc aaatcgaaga tcacgacggc acggcgcatg aaaaacaccg gtgcacgcat
     5161 caagaagacg agtgtgaaga tgttcaacat cctccgccat ccgcagaagg tacgtcgggc
     5221 acatcggacg acgaacaagt aacacgtttg cagatgaaag ggtttgttgg tcatgtatat
     5281 ggtatcgtta aaacaggctg cgggcattgc ccgggaagaa atgacgtccc tcgacgccat
     5341 tacagcggaa ttgctcaatt tcatacagtt gaagagtcag cgcttatata tgcattccct
     5401 gcaggtcgct aactacagcg tcagcgtcgc cgccaaactg acgctgccca agagtgaaat
     5461 cgaacaagtg aaatacgcag cactcctcca tgacgtgggc ctgctcttcg tgtccaatac
     5521 cctgctggcc aagatgccct atctcaaccg ctcggaaaag gcgcagtaca agcgccacgc
     5581 cgcagccggc ggcaacatgc tggaaaacat tccctgctgc caggacatcg tgccttacat
     5641 ccgctatcac cacgaacact gggacggctc gggctttccc aaacacctgc gcggcgccaa
     5701 tattcccttg ggggcgcgca tcatcgccgt cgccgattac tacgacatga tcatcaaccc
     5761 gtcggcagaa ttctgggcga aaaccaaaaa acaggccgtc cgcgaactct tcagcgcgtc
     5821 gggcctgctc tttgatccgg aagtggtcaa agcttttatt gaaacattag gttaataaca
     5881 cattgtaatc aaaaagcgct gttgcatgaa gacagcgctt tttacgttca tcgtaggtcg
     5941 ttcatcgttc atcgaattga agaacgccgg ccgcctgcga actgcgttta ttttatggtg
     6001 caccgctggc gatgacgagg cccgtcacgc tggcggcgcc tttttgtttc aaggcatgag
     6061 cgcaggcttc taaggtcgca ccagtcgtga agatgtcgtc gacgaggagg atgtgttttt
     6121 tatcgacgtc gatggtttct ttgacggcaa aggcatcgtg gacgttctcg gtccgctcgt
     6181 cccgccggag ctgccattgg actttcgtcg gccgcaggcg ctgcaaggcg tcgcaccagg
     6241 tccagcgggc cgaggcccag tcgcgaaaga gggcttcggc ctggttgaag ccgcggcgct
     6301 gtaatttttc cggtgccagc ggcacgggga cgacgacgtc gatgcgggca aaccgttccg
     6361 gccatgggaa gcagtcgagc agggtccggc aggcccggca cttccctggc ttgccgttat
     6421 atttgatgtc gtgcaggaca tggcgcatgg caccgcggta atcggccagg cagtaacagg
     6481 cgctgaggtg cctcacagcc ggcgtcttgt tgatgcgccg gggatgccag atggcggcca
     6541 ggcaggccgg gcaccagtcg ccatcatggg ctactatggc gccgcaggac ggacagcgcg
     6601 gcggatataa gaggttgaaa agctgcgtga tcagggacat ggtcttccct ccctagacga
     6661 tgagttctcc ttgcaggcgc gggatgagca gtgtgaagcg gcggctcgtg cgctggttct
     6721 ggacggctgt ctggacggcc cgcttggtgc cgacgaggat gactttgcgc ttggcccgag
     6781 tgacggccgt atagaagagg ttgcgctgga gcatgatggc gtggctgttg accaggacca
     6841 tgatgaccgt gtggtattca ctgccctggc tcttgtggac ggtaatggca taggccaggg
     6901 tgatttcatc gacttcctgg ccttcgtatt tgacatcctg ttcaggatag cggacataga
     6961 ccatgtcctt ctggatggca aagatttcac ccatatcgcc gttgaagacg cctttttcat
     7021 agtcgttgtg cttctgcatg accttatcgc cgacgcggaa gacgacgttc ttccctttca
     7081 gttcggcctt cccttcggcc ggcgggttga accgttcctg gatgagctga ttgaggttat
     7141 ctacgccaca ggggttctta tacatcggcg acaagacctg gacggcgaat ttatcgcccg
     7201 tttccaataa ttcgctgtta tagagggcgc ttatcttttc ggcgccgtcg gcttcgttgt
     7261 cgatttcgac aaagcggaat tccgtgtctt cattgacgac gggcaggcgg ccgctgttga
     7321 tgagatgggc attggtgacg atgcggcccc cttctttctg acgaaaaatc gtatccaggc
     7381 ggacgaccgg gacctgaccg gacgcgatga tatcgtgcag gacggcgccg gccccgaccg
     7441 atggcagctg gtcggcgtcg ccgacgagga tgcagcggca ctgcggcttc agagcgctga
     7501 ggagatggta catcatttcc atgtcgagca tggatacttc atcgacgatg accaggtcgg
     7561 ccggcagcat cttcgtttcg ttgtattcaa agacctgcat ggccccgacg tggccgtcgg
     7621 gaacgagcag gcggtggatg gtcttggcct tgcgctgcgt cgtttcggcc aggcgcttgg
     7681 ccgcccgtcc cgtcggtgca caaagcagga tgcgcaggcc ttcctgttcg gccaggcgga
     7741 tgatggtctg gacgacagtc gtcttgcccg tcccagggcc gccggtgatg acggtcatgc
     7801 cggactgcag ggatttctct acggcttcgc gctgcttgtc ggccagctcg aaatggcgcg
     7861 catcctgcca gcggtcgagg aagagctgga cgtgagtctt catgggcagg ggcttcatat
     7921 tccccatttc gccgatgcgc ccggcaatat attcctcttc ttcataggct tccggcgtgt
     7981 agacgtacaa agtcccttca aaatcggccg tacagagctg gcccacttcg atggcttccc
     8041 ggaggatgtc gtgcagcccc agggcatcag cctggaggat gaaagccgcc cgccggacca
     8101 gttccgtatc tgggatacag acgtggccat tctgggtcat cgtccgcagg acataggtca
     8161 ggccggcgct gaggcgctgc gggtcctgcc ggtccatgcc gtaggccagg gcaatctggt
     8221 cggccgtctt gaagccgatg ccgtcgattt cagcgatcat gcggtacggt tcttccgtca
     8281 ggacgtagtg gacgtcgtcg ccgtatttct gcaagagccg gctggcataa cggccggcga
     8341 cgccgtgctg ttccaggtca taggcgatgt cgttgacctg tttttcttca tagaaggatt
     8401 ccgtaatgac agccagcttc ttagggccga tgccttcgac ttccaggagc cgctgcgggt
     8461 ctttttccag tacggccatg gtatccttgc caaaggctgc gacgacgcgg tgggccaggg
     8521 ccggtcccat gcccttgacg gcgccgctgc ccaggaagcg ttcgatgccg tcgaccgatt
     8581 cggggatgat gcggctccag ccgtcagcgg cgaactggcg gccatagcgc ttatgctgga
     8641 cccagtggcc cttcacttcc aggcggtcgc cgacgagggg tttcgtcccg tggccggtga
     8701 cggtatatgt cgcatcatcg tcttcatcgt gcaggcggaa gacgcggtaa tccgaatcgg
     8761 gcgcttcaaa taagaggcgt tccgcaatgc ctcgtactac gatttgttcc atcttcacag
     8821 ccccatatgc tcccgttcgt atttcttgct ctcatccatg atgcactgat aaatacgccg
     8881 cagatacggc gacaattcct tctggacggt cagattggcg atcttatcca ggacctgccg
     8941 ttcccggcgg gggtcataga tttccatatg atgctcctcc ttataggcgg cgacttcagc
     9001 gacgacctgc atgcgcc
//
LOCUS       DAWABZ010000072         9016 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2090_length_9016_cov_2.631180, whole genome shotgun sequence.
ACCESSION   DAWABZ010000072 DAWABZ010000000
VERSION     DAWABZ010000072.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 9016)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9016)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9016
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2090_length_9016_cov_2.631180"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1865 a         2343 c         2607 g         2201 t
ORIGIN      
        1 gcttcgctga cagctctcct ataaggagag ccacgagcac gagccactaa tcttttttgg
       61 ctgcgatgcc gtagggaatg gacaggaaga ggacggcacc gacgatgttg cctaaggtga
      121 cgaaggccag gttgtagagg taaccgccga cgctgacggc cgtaccggcg ggattcagca
      181 ggcccagggt caggacggtc atgttggcta cgctgtgttc aaagccacag gtaaagaagg
      241 cgtagaggca ccagaagacc atgatcagtt tgccgctgtc cgatttgcag cggaagccgc
      301 accagacggc caggcagacc aggacgttac agaggatacc tctgaggaag aggaccagcg
      361 gcggcaagct catcttcatg gcagcggcat tggccaaagc ctggccgacg ccgccagccg
      421 tgccggcagc accatagaag agcagggaca ggacgatggc gccgaaccag tttcccaggt
      481 aacagacgac ccacaggcgg atgacgtccg tcaaggtgac cgtcttcgac cgccagccat
      541 tgtagaggac gaaggcgtta cccgtgaaca gttccgcccc ggcgatgatg gccaggctca
      601 gggcgatgcc gaaggacagg cccatggcga tcttagtcat gggaatggct cccaaggtcc
      661 cgctgaccgt aaaggacagg aggatgccaa agccgatata ggcaccggct aaggccgaca
      721 acaggaagta tcccagggga ttcttcttca agaggcctac tttcgcagct gccccggccg
      781 cggtcgcagc gtattcgtcg ttgtacatag gctcctcctt atttcagcgg caacggtttc
      841 tggaatacat ccagatcggc cggatttacg tctttgccga cgtagaacgg ttccgatttc
      901 aagccgacat agcggatacc ggaccagtta agaggacggt agatttccgc cttcttcggc
      961 agatggacgt cctggaggat gaacttgatg aattcttccg gacccgtacg gtctaccatg
     1021 tagccgatat gttccttgcc acccggagcg gtcgggtcga tatatttctt ggcaaaagca
     1081 tagacgttgg agataatctt cacgacgctg tcttcatcgg cccatttgat gaaatcgacg
     1141 cccatgcgcg ggttcttttt gcctgtacgg cccaaaaggg tgatacggaa atactgttcc
     1201 ttgctgcgcg tccaggcacg gttcgggcag gcccagacac agacgccgca gccgatgcat
     1261 ttttcgacat tgcggacagg acggaaattc ttcatctgca aggcttcgac agaaagctgt
     1321 ttacacttgc ggacgcacat gccgcagctg acgcagcggc tggcatcgaa ctgaggttcc
     1381 gtcatgccca tgacgccgaa gtcatgcatg cggattttgt cgcagtcgtt gggacagcct
     1441 gtaagggcga ctttgacgtg caggtcgttc gggaaaatag ccttttcgat ccgtttcgcc
     1501 agggccgtcg tatcgtagtt gccaaagggg cagacgcggt tgccgacgca ggccatgaca
     1561 tttcgggtac cggacgcttc aaaaccagcg cctcgttcgc tctgattgat gtcgagttca
     1621 tcgatgatcg gctggagagc cttctttact tcttcaactt tttcaaaggg aatccccatg
     1681 atttcaaagc cctgacggat ggtcagatgg atttcgccat taccgtacgt ttcggcaata
     1741 tgctggacct ggcccaggac cttggcattc agggaaccgc cgggtacacg tacacgggct
     1801 gaagccatat tacgattttt tgttacccgg aaagcgtatt tctttaactt accgcaattc
     1861 atatccataa tgtatcagcc tcctagtcga tcaatgtacg aagttcggtg tatttgaata
     1921 ccggcccgtc ctggcagacg tagactttgc caatacggca gtggccacat ttgccgagac
     1981 cgcagcacat tttgcgcgat tcagagagcc agattttttc ttcatcgaaa ccgagtttaa
     2041 tcaattcctg gatggagaac ttgatcatca tcggcggacc gacgacgacg gcgatggcgt
     2101 tgtcgaagtc cttgaggggc agttccggga tatactgggt gaccaagccg acatggccgt
     2161 cgtaatcccc ttcttccgga gccttgtcga cggtgcagat gaagttcatg ccgtcgtcaa
     2221 tccagcgttt gaggtctgct ttgaagagga tgtctgccgg cgatttgaaa ccggagatga
     2281 cagtcaggct cttgcattcg tccatgtgat cggcgaagta gtcgatgacg ccatggaccg
     2341 gggacaagcc ggagccgcct gtgaccagga cgatttcctt gcctttgaac atattgacgt
     2401 cgaagccgtt gccataaggc ccgcgcatga agaagtggcc gccgacgtag ttttcaaaga
     2461 tttcatcggt caggctgccg actttacgga tggtgaaatc cagggaatgg tcggtaatgc
     2521 cgctgacgga aatgggcgct tcaccgtatt tcgggataga gatttcaaag aactggcccg
     2581 gtttgaccgg atgttctttg ccgcggtcat aggacatacg gaaggtatat tccacatcag
     2641 catgtttaat gacttctaag atttccgaac ggaacggtaa atattcgtta ttcattctct
     2701 gtgacctcct tcatgccttc cggcagtttg acattgatgc aatggctgag cgaaatgtat
     2761 tccggacaga tggcatcgca gcggccgcag cctacgcaca tctggaaacc ggaacgctgt
     2821 ttgaattcca gcgtcttatg gagcactttg aagcgcatcc gttcgcccgc cttcttgcgg
     2881 aagcaaaggc caccggcaac cagggtatag tcgtcgaaca tacaggacgt ctgtacacgg
     2941 cggcgttcac cggctttatg gtcttccgtg aagaacacat cctgcatgga ccagcaggta
     3001 caggtcggac aggccagggt gcagcggccg catttgatgc agcgcttgtc gtattcatcc
     3061 catacgtggg atttggcgat agccgacgag aggttttccg gaatgtgtac ctggagcttg
     3121 ttttccgtca catggtccgg gacgacgtcg acttccggtg ccttggcttc gctgacagct
     3181 ttcccaagga cgtcatcttt gacatcgagg aaatactggc cgtctttgac gttcaaggcg
     3241 acatcgtagt tatcgctggt attggtgccc atggaaacgc agaagcagtt gtcgaattcc
     3301 tggctgcagc ccatgaggac gaatttgacg cggtcgcgga cgcgtttgta ataatagtct
     3361 tccggaccgt tctggaggta catgtagtcg aagcgcttga tggcgtgcaa gtcgcagctg
     3421 cgcagcaaaa tgatggcacc cttttccggg gcttcggctt cggtgacggt attttcattg
     3481 aaatagaaca acgtctggca aacgggcaat aaaacgtctt ttgccgagta gtcggaccgt
     3541 ttgtcccaaa cgatttcttc cggcttggtg atcttgccat agcgtacgat gtcctgcatc
     3601 gaagaacgtc ccgtgccggc aaacagtttc ggagcgtaaa tgtcatacgt atccgaccat
     3661 ttggcaaaga ccgtgttcaa tgcatctgca tttaattgat agcccatgct gttaccctct
     3721 ttctaaagaa ataaatgaca aatataatgg ataaacaaat tatttttttc acatacaccg
     3781 tgacgacatc ataagccgcc tcgttgagtt tatgataaca gcgggagaag aaaaaaaccg
     3841 ttgccgtagc aacagttttt ttaatttttt tctaaatact atgctttttt atcatctaaa
     3901 atcgttatcg ttgaggaatt taaatacatt atacttttcc ccataatctt ttcttttcag
     3961 gtcctatgac tcatcataac gctgtaactt attaccggta gtccatgctg tctttgcata
     4021 cagaaaaaga ggctgtcgcc tgaagacagc ctctttttga gtgttaataa tcattcaaaa
     4081 tcgttgtgga atttaatctt gcgatacgtt tcaaaagtca tccacttgtc gaagagttcg
     4141 gcatcggagt ttcccccggc cttggcgtag tccgccttga aggcagcgaa ttcctgcggc
     4201 gaatggacgt tgatggcaaa gccgcggccg ttgactaaag gctgttccgt ataggcatag
     4261 ccgtcgaggg acaggatgtt ggcgactttg gcgtcgcgga tttcgacggc aaccttgtcg
     4321 cggaggatga ggatgtcgaa gttatccgga taggcatagg tttcgccttt cgtcgggatc
     4381 gtatcgccga tgtggtactg gtggaaggcc ggtttggcct tggtgatatt gaggctctgc
     4441 atgccgtaga agaagtcttc aaagacgtcg ccgcgggcct tcagggagcc gttgacgaag
     4501 gtgtggtcgg cgttggtgtc gttaacgtcg atgacgacgc cgcaggccga ggtcatattg
     4561 gtgacgcggt cgatgaggat gagttcgccc aggaccttgt gatgactgaa ggtatcgacg
     4621 acaatcggtt cctgcaggaa gagcgtgcag acggcgattt cgttcttcgt aaggctcgaa
     4681 gcgcgtttct tttcgcctgt attgacgtca atggtgtagt cgatatcagt gaggacgccg
     4741 acgatttcct tggtccccaa tttgacgacg tattctttgc cgacagccag gtcctggtca
     4801 tccatccaga gcatggtcgc tgtaatgcgc tgactcaggg cgacgtccgt ccccttggta
     4861 aagacgcagc cccgggatac gtcgacttcg cggtcgagct ggacgttgac cggctggcct
     4921 ttgacggccg aatcgacggt atcgaagccg atgaggatgg atttgacctt agcttcttcc
     4981 ttgctgggca gggtcgtaat cgtatcgccg acggccaagg cgccgctttc gatctggccc
     5041 tggaagccgc ggaaggtgtg gttcgggcgg cagacgcgct ggatgggcag gtaaaagcct
     5101 tcttcttcgt cttcttcgac gttgaccgtt tccagatagt cgaggatggt cggcccctgg
     5161 taccagggca tattggccga ggccttggta acgttgtccc cttccgtcgc cgagacaggg
     5221 atgaggatga cgttggccag ttccaattca tcgcggagct tggcgatctg gaccttgatt
     5281 tcttcaaaac gttcctggct gtagccggcc aggtccatct tgttgatggc gaagatgaag
     5341 tagcggattc ccatgagggc gcagatgcgg gcatggcggc gggtctggac caggacgcct
     5401 ttggtggcgt cgatgaggat gatggccaag tcggcaaagg aagcaccgac agccatgttg
     5461 cgcgtatatt cttcatggcc cggcgtgtcg gcgacgatga agctgcggtt gtccgtgctg
     5521 aaatagcgat aggctacgtc gatggtaatg ccctgttccc gttctgccat gaggccgtcc
     5581 aggagcagcg agtagtcgat ggccccgccg cggctgccga ccttgctttc cagttccaga
     5641 gccttcttct ggtcggcgta gagcaatttg gcgtcataga gcaaatgacc gatgagtgtc
     5701 gatttgccgt catcgacgct gccgcaggta ataaatttta acaagccgtt tccgtttcta
     5761 tccattttag aaatacccct ctctctttct gcgttccatg ctgccagctg cttcattatc
     5821 gatgacgcgg ctcgtccgtt ccgacgatac ggcgctcagc gtttcatcga tgatggcatc
     5881 cagcgtatcg gcatcggatt cgatgccccc tgtcaaagga tagcagccca aggtccggaa
     5941 gcggatcttc ttgtgcatga tttcttctcc cggcttgagg cgcatgcggt cgtcgtcgac
     6001 catgatgatg ttgccgtcac gatagacgca gggccgttcc ttggcaaagt acaaagagac
     6061 gatgtcgatg ttttcccgtt tgatgtactg ccagatatct ttttccgtcc agttggaaat
     6121 ggggaatacg cgcatgcttt cgcctttatg gatgtgcgta ttgtagagct tccacatttc
     6181 cgggcgctgg ttcttcggtt cccaggcctg ggcttcattg cggaaggaaa agatacgttc
     6241 tttggcgcgg gatttttctt cgtcccggcg gccgccgcca aaagcagcgg tgaagccgta
     6301 tttgtgcagg gcctgcttga gggcctgggt cttcatgatg tccgtatagg ctgcgccgta
     6361 atcgaaagga ttgacgccct gtttgacgcc ttcctggttg atgtattcaa tgagctgcag
     6421 gccgtacttc ttcatggttt catcgcggaa gcggatcatt tccttgaact tccacgtcgt
     6481 attgacatgg aggaacggga aaggcggctt ttccggataa aaggccttcc gggccaggtg
     6541 aagcatgaca gaactgtcct tgccgatgga atagagcatg accggctttt cacattccgc
     6601 agcgacttcg cggatgatgt aaatcgcttc ggcctctaac ttgtcgagat gcgataatgc
     6661 acacataata tgaatccccc tattgataaa atgcagtttc cagtttatag tctgcagttt
     6721 gccgtaatgg gtgctctttt actacaaact cgctactaca aacggcaaac taattaatat
     6781 gaattcgcct gtttgcggtc gacggtgatg gtcttttcac tgccgtcggc cttatggata
     6841 tgccaatgca ggtagtggcc gtcagcagtg acgttcatcg tgctgccgcg gccgcccata
     6901 tcggcgccga ggtagaagtc gatggcctgg accgggcaga ctttgacgca ggatacacag
     6961 ccccagcagt ccttgggata atgcatccag gccttgccct ggccgttgcg gtcgatgagg
     7021 ctgcccgggc agacgtcgat gcactggccg cagccgatac agcgttcctg cttaatgcgt
     7081 atgctcataa gtttcccctc ctggtactaa atcgcggtag atcatgtgga cctggccgtc
     7141 ttcgtccatg cgggaattga cgtatttcag ccaatccgca cgctgttccg gataatcgag
     7201 gttctctgca aaggaatgcc accgcgtttc cttgcgggcc cgcaggtggg cgatgaggac
     7261 cttgcagacc gtcagccgtt cgcgcagttc gtagacgaag agcagttcat ggtaatcgct
     7321 ggcgccgacg tgggcggcca gttccatgag ctggtcgatc ttttcatcgg ccagggccag
     7381 ctgcttttcg ttgtactggt aatggctgcc gatgccgccg gcgtaggtat ccatgacttt
     7441 ctgcatggct tcttccagct gttccacagt gaagaagctg tccgtctcac cgagggcggc
     7501 gttgtattcg gctacctttt cggcgaggat ggctttttcc tgggcttcat cgaggccgga
     7561 tgcatcgctc aggccctggc ggaccatatc cttggcggcg atttcccctt cgaccagggc
     7621 accagtgacg tatttctgcg gacagccgcc ggcgacgtcg ccagccgcat agaggtggcg
     7681 gatcgtcgtc tgacggttcg tgtcgaccca gtagccgctg gccgtatggc cgccgacgac
     7741 gtacggttcg gtcccttcga tttcgacgtt ctgctgactc ggcaatttcc ccgactcgat
     7801 ccatttcaaa gtctggctcg gcgccatgtt gaggtaggcc ttgaggagcg attcgtcctg
     7861 agccggcgtg atgccttccg tgcggagata acagggaccg ttgccgtcca gcgtttcccg
     7921 gaccgtgccg tacaggcgct gggacgtggt caggccgtat ttctgttcat atacgtcacc
     7981 gcgggcgttg acctgcttgg ccccgacgcc ctgggcgata gtccctgtcg gcgcgatggt
     8041 atccttgcag cgcagggcga tgaagcgcat ttcaaaggtc gtcatttcgg ccccggcccg
     8101 gatgcccatg gcatagccgg caccggtgtt gaagggacag taccacatct tgtgccggga
     8161 aaaaccggga ttgttgggcc ggtagaggcc ggctgcgccg cctgtggcac agaggacggc
     8221 tttggcccgg atttcatagg ccgtgcccgt gcgcatatcg aagcccaggg cgccggccat
     8281 ggtattgtcg cggacgatgt agtcggtaat attcagatgg ttgatgacct ggacgccatc
     8341 gagcttcttg acggcgtcgg ccaggatggt cttgatattt tcgccgttga tcttgatgtt
     8401 gcgcgtgccg cgggcgacgt attcaccctt ttcgtccttc aaaatgacca gtcccaggtc
     8461 ttccagcttc ctggtgactt cgttgaggcc ttcggacatg gtgaggagca agtcttcccg
     8521 gacgatgccg gctgcgtctt tcttggcgta gtcgacgtag aactgcggcg tatggccctt
     8581 ggtgatatag gcattcaggg cattgacgcc ggctgccaga cagccgctgc ggcggatatc
     8641 ggccttttcg gcgatgatca cggacgcatc agacaggctg cggagggtca gggcggcata
     8701 acagccggcc gtcccgccgc cgatgatgag gacatccgta tggatgatct tctcgctggt
     8761 aatctgcata acatagactc cttcgttaaa tatagaaaac gtcggccgag gtcatggcgc
     8821 tgttttcttt taaatacgtc ttgtcgccgt cgaggacgta caagcggtaa atgaggacct
     8881 ggacggtctc gccgacagac agcggcgggt cgtcgaagga atattcgccg tggatggcca
     8941 ggcccttgat gcggacggtc acgtcgaggt ggctgccgcg gaagacgacg gcttcgaccg
     9001 tcccttctgt attccg
//
LOCUS       DAWABZ010000073         8989 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2099_length_8989_cov_3.238639, whole genome shotgun sequence.
ACCESSION   DAWABZ010000073 DAWABZ010000000
VERSION     DAWABZ010000073.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 8989)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8989)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8989
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2099_length_8989_cov_3.238639"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2012 a         2566 c         2546 g         1865 t
ORIGIN      
        1 gagcgcacgc ctgataagcg tgaggtcggt ggttcgagtc cacctaggcc caccacacag
       61 accatcgttt ggattattcc ggaacgatgg tttttttttt caacagaagg agggaagacc
      121 atgacggaaa gtgaaaggca gctccaggaa atggccctgt ctatccaggc ggcccggcag
      181 caggccgggt gcagggcggc cgtcacggca ccaccgacag cggccgatac gattcccaat
      241 ctcggcggtg aagctcgggc aggagactgc ctggtcagca tgctggccag cggcagcaag
      301 ggcaatgccg cttatatccg ttgtggcaag acgaatatcc tcatcgatgc cggcatcagc
      361 tgccgccgca tcgaacaggg attgaagcgc tacggctgcg ctttagggga cctcgatgcc
      421 gtcttcatca cccatgaaca ttcggaccac gtcaacggcc tggcgacgct cctcaagcgg
      481 acggacatgc ccgtctatac gacagccgag acctggcagg ccataggccg gaaagtggaa
      541 ggcttccagg accgcttcgt ccgcctgaca cgccgcgtcg gcctgaagga tatccaggtc
      601 attccctttg ccatatccca cgatgccgtg cggcccgtcg gctatacggt ctgccacggc
      661 gaatcgaaga tcaccctggc gacagattta ggctgtgtca gccctgacgt cgcggcggca
      721 gcggcttatg cggacatcct cattttagaa gccaatcacg acgaaggcat ggtccgcaac
      781 gggccctatc cctatccgct ccagcagcgc atcctcggcc gctggggcca tctgtccaac
      841 aagacggcag ccgctttcct ggccggtctg ccgacgaagg gcgtcatgcg ggtcctcctg
      901 gcacaccgca gcgaaaagaa taacacgccg gccctggcgc tgcacacgat gcgcactatc
      961 ctgtctcagg ccggacgggt catcggccag gatattttat tgcggttggc ctgccagaag
     1021 ggcagcgtcc gcttccagga aaggagtcat gaattatgat gcaacgcaaa catgtcatta
     1081 cggcggcagc cgccgtcgtc ctcatcgccg tcggcgtctt cggcggtttc aagatccacg
     1141 atgccttttt tacgccgagc ggtacctata acgggacggt gaaccagctc caggaaccgt
     1201 cgaaatcgga ttccatttcc gatgcccgca atacggccgt cgtccaggct gccaagaaag
     1261 tcaacccggc tgtcgtcggc attacgacga aagtctataa ccgcgatatg ttcaaccgca
     1321 aagtcctggt cggtgaaggc gtcggctctg gcgtcatctt cgacaaggcc ggctatatcg
     1381 tcactaataa ccacgtcgtc ggcacggcca agacggtcat cgtttctttg gccgatggcc
     1441 agagtaccga agggactgtc gtcggccgcg atgaaaagac ggacctggcc gtcgtcaaga
     1501 tcaagatgga caacctgccc gtcgctgaat tcggcgacag cgattccctc caggtcggcg
     1561 taccggccat tgctatcggc aaccccctgg gcctggaatt ccagggcaca gtcacagtcg
     1621 gcgtcatcag ctccctcaat cggaccatcg gggctgaagg ccagagcatg aagctcatcc
     1681 agaccgacgc agccatcaat ccgggcaatt ctggcggcgc cctcgtcgac gccgacggca
     1741 aggtcatcgg catcaacagc gccaagattt ccaaggaagg cgtcgaaggc ctgggctttg
     1801 ccattcctat caacgcagcc cgtccgattt tgcaggacct catcaagaac ggcaaagtcg
     1861 tccgcccgta cctcggctta tacggtctgg accagcagat ggcagcccgt ttcggcatga
     1921 agctgaacgt agccggcatc tacgtctata aagtcgctgc cggcggtcct ctcgaccagg
     1981 caggcctccg ccatggcgac gtcatcctga aactcgacgg cacggatgtc aaagacttca
     2041 gcgctctcca gagcgtcatg gataaacaca acgtcggcga ttcggtcagc ctcgactaca
     2101 cgcgcaacgg ctcggaccac gaagtcaccg tcgtcctcca ggaaagcccg cagaatgatt
     2161 cgtccgacca ggaagacggg accaactgat gaagtaccac gtcattacca tcggcaagat
     2221 caaggagaaa tacctcacgg ccggcatcga tgagttcttg aagcgcctgc gcccgtacgg
     2281 ccaggtcgag ctcaccgtcc tgcccgaaga gaagatgccc gacaacccgt cgccggccca
     2341 gaagacccag gtcctcgaca gggaagggga gaagatgctg cgccacgtcc gccagggctc
     2401 gcacctcttc gctctggacg tgcagggcaa gctcgtgtcg tctgaagacc tggcggcgtc
     2461 ctttgccaaa ctggccctgt acggctgcag cgaaatctcc ttcatcatcg gcggcccctt
     2521 cggcctgagc gacctcgtcc gccgcagagc cgacagctgt atctcctttg gccgcatcac
     2581 cctgacccat cagatgatac gcctcctctt attagaacaa atctaccgcg ccatcaaaat
     2641 cgaccggcac gaaccctatc atttgtaagg ggctggcagc tagcagttag ccgttagcag
     2701 cttgggcgtc tggtttttac gaaccgctaa caatgaataa cttaaacagg gactacgcac
     2761 gaagacttat gtcatcatgc gtggtccctg ttgttttata gttcgtaggg gtcgccgcgt
     2821 gggcgacccg ttggatttgg atgattattc tagcaaactg ggcggtaaat cgtatatatt
     2881 tctagagcgg gctcgccacg tgcgagcccc tacagcttaa aaaaggcttg ccgcacatgc
     2941 gacaagcctt ttttaagctg gtggaccatc aggggatcga accctggaca ccctgattaa
     3001 gagtcaggtg ctctgccagc tgagctaatg gtccatattt tgttgtcctt cgtactcgct
     3061 gtttcctgag cacatgtaat ataatacact aaacctcaga gaaatgcaag cgctttttta
     3121 agaaaattta cagaatcttg aatttccccc gattcttgat tttggcgaca cggcgaatgt
     3181 ataatgaaag aagtgatatt ttacaaaaac tactcgtttg ttgatagatt gaaagtgagc
     3241 gatgtgtatg attacttata atggcaatgg cggcatggac actgtcgctg tcggcctgaa
     3301 tgaacggggg tcgacgctgc gccagctgcc cgaaggggat gtccagctca tccgggccta
     3361 tgccggacag cacccggaga ttttgcagca tacgtccctt cacgtcttcc actgcctgtc
     3421 tgacggccgg gccaggacct atttcctcat cggcttggaa gggaagccca tccgctattc
     3481 gacgtatgaa gatttccgca tggcccggcg cctcatggcc gcagccatga aagaaggcgt
     3541 cagcgaactg gctgtcatgg ccgataccct ggatatggac ctggggtccc tcatcgatgg
     3601 cctgctctac cgtaattacg aattcaatca ctataaacgc caggccaaga gccggactat
     3661 cgggaatatc aatatcctga cccacagcct gcgcccgaag gaattcaatt ccatgtgcta
     3721 catgtataca tccatctatg aaggcatcta cgcggcgcgg gacctggtca acatgccgtc
     3781 caatgatttg acgccgcgca agtttgccga tatcgtcggg gccatgctca aaggcgacaa
     3841 catcctcata gagaatctca ataaatgggc cctggaaaaa cgccatatgg gcggcatcgt
     3901 cgccgtcggc aagggcagca tgaacccgcc ccagctcctg tccattgcct accagggcga
     3961 ccctaacagc cgggaagtcc tgggcatcgt cggcaagggc gtcacttatg acagcggcgg
     4021 cctgtccctc aagagccatg ccaatctgga attcatgaag gacgacatgg ccggtgccgc
     4081 agcggctgtc ggcgtcatgc gggctttgat gctgctcaaa tatcccgtca acgtcctggc
     4141 cgtcctcccg ctggtcgaaa atattccctc cggcatggct tatcacgtcg acgacatcct
     4201 gaccatgtac aacggcaaga cggtcgaagt caagaacacc gatgccgaag ggcggctcat
     4261 cctggccgat gccctgacgt atgctcagga aaagggggcg acgcgtctca tcgacctggc
     4321 gactttgacc ggtgcctgcg tgacggccct ggggaccgtg cggacgggca tgatcggcaa
     4381 cgaccaggaa tggatgaacc gtttcttcag cgctgcgacg gaagttcacg aaaagatatg
     4441 gcagctgccg gctgatgaag aatatgaaaa gttcctggaa agcgacgtcg ccgatttgaa
     4501 gaacgtcggc ggctccgaag cgggagccat cacggcaggc ctcttcctca agcagttcgt
     4561 ccacgacggc acgccgtgga tccacttgga catcgccggc ccggccttca atgaaaaggc
     4621 cgacgaaacg ggcctcatcg gggcgaccgg cgtcggcatc aaatccattc ttcacttatt
     4681 gcagggaggc gtggcgtccc atgatcgttg atttacatat gcatacgact tgttcagacg
     4741 gcgtatatac accggaacaa ctaacacaga tggccgtcga cgccgggctc agcgtcatgg
     4801 ctatttccga ccacgatacg gtagcggctt atgacggcag ccatcgtttc gccgcaggcg
     4861 tccgcgtcat cccggctatt gaaatcagct cggaatgtga cggcgaagat gtgcacatcc
     4921 tgggctacgg catcgacacg gctgacgccg gcttgcaggc ttactgcgca caattcaagg
     4981 aacggcgctt caacagggcc ctggaaatcg tcgaccgctg ccgcagctta ggctatgaca
     5041 tcaatcgcag cggcatcgaa gcgacactgc gccagggcgg gactgtcggc cggccccaca
     5101 ttgcccgcct gctcgtggcc aagggctatt tccccgacgt caagacggtc ttcgataaac
     5161 tgctctaccg cggcggtccg gcttacgtcc cttatcaccg ccgcagcatc gacgactgcg
     5221 tggccctcat ccgccaggcc ggcggtacgg ccatcctggc ccatccgggc ctgctcaagc
     5281 ggacgctgga ccaggtcctg gcccatgact ttgacggcgt cgaagtctat catcccaaga
     5341 accgcggccg ctataaagaa ttcttgaaac tggctgagga ccgccatggg tacgtcagcg
     5401 gcggctcaga tttccacggc acgacaggcc gtttccccga aaaggtcggc gtctttaccg
     5461 tcgatgccaa ggcggtaaaa gctctgctcg accatgtaga agggaaccta aagaaaagca
     5521 ggtgagatta tggaggtttt cggttttatc ggcgccagcg gcacgggcaa gagccatcac
     5581 gccctggtcg tcgcctatga ccacgatatc caggccatca tcgacgacgg cctgctcatc
     5641 gaccacaacc gcatcgtcgc cggccggtcg gccaaagaag agacgaaccg cctgaaagcc
     5701 gtgcgccgag ctatcttcaa caagcccgaa gacgcagcgg ccatgcgggc ggcgctggag
     5761 aagatacatc ccgataaatt gctcatcctg ggcacgtcga agcacatgat acaccgcatc
     5821 tgtgagtccc tgcacctgcc ggaagcgtcg cagttcatcc gcatcgaaga tgtgtcgtgc
     5881 ccgtcggaaa tcgccaaggc ccgggccatc cgcatcaaag agggcaagca catcatcccc
     5941 gtgccgacga tggaactgaa gccccatttc cagggctatc tcctggaccc gatccgttcc
     6001 ttcttctatg gccgcaacgg caagaagata tcgccttttg aacggtccgt cgtccggccc
     6061 gtcttcagtt attacgggaa actgaccttt tccaacgacg tcttggaagc cctggtgcgc
     6121 catgccctgg ccaagaacga cggcgtcacc aagatcaacc gcctgcgggt ggccatcaat
     6181 tacaactcga cgcgcaatgg cctggccatc atcctgtccc tgaccatcgc ctatggccag
     6241 aacatcaaga agctcatgag ccgtatccgc cgggacatcc agcaggaagt ggaatacacg
     6301 acgggcatgt ccgtcgagat cctcaaaatc acggtccgca gcatttccca gcctgaagac
     6361 taggcgtcgc cgttttcttc ggtcaggacc atcatgagcg gcgtaatcag gccccgcagg
     6421 gcgtcgacat tttccgccga gaagcccttg tactgaggag aatggctcag gccgtcgaag
     6481 aaatcatgca tacagccgat gagctgggaa ttattgagca agtaaatggg cttggtctgg
     6541 ggcaatatgt ggtgcgtgtt ctttttgaag aaagctgacg tgtgattgcc gtaaaaagcg
     6601 agattagaaa aattcgtata actgctttct cccgatgccg ggaggagaat cccgtaagta
     6661 atggccgggt tctgcttcat cttttcgacg atgaggttga gctgggcctt tctttcgtct
     6721 ggcgtcatct ggtagttcat gtcggtcagg tagaagcagc ccgtttccag gtagcggatg
     6781 atggaattct tgggcatgat gattttcatg cgcccctgtt cgatgatgcg gtaccagatg
     6841 tactggatgc gccggaccca ttctttggtg gcattgggat attttccttc gtcgatgagt
     6901 tggcacagcg acgtatagac ttcgtcgggg aggaggaatt ccaggccgtt ggtcaggaag
     6961 aagaggtagt tgttggatgt aaagaagatg tcgcggtagc cgaatttttc catgcccagg
     7021 gactggcggg gctccatgag gacgggctgg accatcatgt aggcgcagca cttcttatag
     7081 atttcccgga cctgggccgg gtcggtgatg gtcaggcaca tgtcgatgta gtcgtcgccg
     7141 tcgacgtagt agaggtaggc aaaggcgtct ttgaggacga tgatgttgcg gtacgattcg
     7201 tggtcgattt cgtgcatggt gaaattatag gtcaataagt ggtccagaca ctgatagagg
     7261 ctgctggcgg cctggggcat atgatcggca aagacgtcca tcttcaggcc taagtggacg
     7321 tcgcaggacc agcggtcgtt gtgacattct tccaggagct gccagatgcc gttctggtcg
     7381 aggacgtgga actggccggt catgaccagc attccctggg actggaggcc ttccaggtat
     7441 tggtggagca gctggcacag ggccgtgtgg cagtcagcgc ggccggcgta gaaatggatg
     7501 gcctggtcgc ggctttcact tttccccttc ctggtctggg cctggcggta ggcacggctt
     7561 agcaggtcgt agagatggaa ggccagatcc tggccgtcgt cggcaaagga gaagatgcgc
     7621 ttcaggtccc cttcctggtg ctgtttctgg gccgtgttgg caaagaagcg ggccatgtcc
     7681 tcgtggatgc ggtcggcatg gcgtgaagcc ggcaggcggg cgccgttgac ccatttgcta
     7741 atgtaggaca tgtcgtagtc gatggcctgg gataaggtcg tatatttcgt ctctgtatat
     7801 tgcagcagct gcttgaggac ttctgcataa ggattcatga acaggactcc cttgatgata
     7861 gatttgaata cgtgtatatt ccttatattc cttatattca ttatatagcc tggccaggac
     7921 accttcaagg aaaggccttg tcatatttgt catctttgag gcaagttttt catcttttgt
     7981 cacggctttg tgcgtatttt tcaagggcaa aaggggctat actaaagcca gaagatgaga
     8041 agaaaaagag ctcatcgaag acaagtaagg aaatctataa ggaggaagtc atcatggcaa
     8101 aactgattac agcagaaaat gcagcatcgc ttattaaaga tcgttcgact atcgccgttt
     8161 gtggttttgc acagtgtgca ttgccggaag aagtattgca ggccctggaa gaccgcttta
     8221 ccaagaccgg atatccgcag cagctgacac tggtccatac ggccggcatc ggtgacggtg
     8281 tgagcaaggg tgccagccat ttcgcccacg aaggcatgct gaaacgggtt attgccggtc
     8341 attacaacct ggctcccaaa ctggggaaac tggtcatgga caataaggtc gaaggctaca
     8401 acctgccgca gggcacgatg gcccagtggt tccgcagcat tgccggccgc aaaccgggcc
     8461 tgtttacccg tgtcggcctc aataccttcg tcgacccccg catcgaaggc gggaaattga
     8521 acagcatcac taaggaagac ctggtagaag tcaaggaact ggacggcgaa gaatatctct
     8581 ggtacaaacc gttccccatc gacgtcgcta tcgtccacgg gacctgcgcc gacttgaatg
     8641 gcaacgtatc gacggataat gaagaagtcc gcatggaact cctgcccatg gccctggcag
     8701 ccaaagccag tggcggcatg gtcatcgtcc aggtcgaaac gctggccgaa aaccggacga
     8761 ttccgccgaa acacgtcgtc ctgccgggca tcgacgtcga ctacatcgtc ttgtccaagg
     8821 tccgtcaggt ccagaacccg gcttataccg gtgaaatgcg tatgcctttg tcccagattc
     8881 cgccgatgga actcaatgcc cgcaaggtta tcgcccgtcg ggctgcgatg gaactcgttc
     8941 ccaacgccgt cgtcaacctg ggcatcggga ttcctgaagg tgtttccag
//
LOCUS       DAWABZ010000074         8962 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2106_length_8962_cov_3.088582, whole genome shotgun sequence.
ACCESSION   DAWABZ010000074 DAWABZ010000000
VERSION     DAWABZ010000074.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 8962)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8962)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8962
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2106_length_8962_cov_3.088582"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6506..6515
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2320 a         2459 c         2286 g         1887 t
ORIGIN      
        1 tttaggcccc ctcccctata aggggaggca taccgacgaa cgcatcgccg aaggcgataa
       61 gccacgaacc actaaccact aaccacgggc tcgctacgcg agcctacttc aaaaggagtg
      121 atacaaatgc catatcgtat gatgttagct gtcaacttta caccggaacg gctggaccag
      181 gtcaagctcc tggccctctt gacgaagtcg aaggtcaagg ctgttacgga agcggaaaaa
      241 ggcgaaacag tcggcaaagt cctgggactg actgacgaag aaatcgctga aatcgccgct
      301 atgaaacagg aaacggaaca gaccaaggtt cacgaacaga tgaacgaaga cgaaaaactt
      361 ccgcctgtca ccaaagaagc gctgattctc tgcggcttcg ataaccaggc tgtcaacctg
      421 ctcctgagca gcatccgccg gggccgcctg aagaacgtgc ccctcaaggc tatgttgacg
      481 ccgaacaaca tttcttggac tatcgaaacg attttgcagg aattgtcgaa agaacacgaa
      541 tattttcata aaaagaggta agaccatgcg tcctacgaca ctttgctttc ccgtccgccc
      601 ggacgggaaa cttttattag gccgcaaaaa acgcggcttc ggcgtcagca aatggaacgg
      661 cttcggcggt aaaatcgaag ccggtgaaaa cttcctgcag tgcgccgtcc gggaactccg
      721 ggaagaaacg ggtctcatcg ccaaggaaga agacctggaa ctcgtcggct tcctggactt
      781 tcacttctcg gcctcatcgg acctggacca catcggcttc gtctacttcc tccgccaatg
      841 gcaggggact gtccaggaaa ccgaagagat ggaaccgcaa tggttcgacc cgtcagccct
      901 tccgtatgat gaaatgtgga aaggcgaccg gacctggatt cctatgctgc tgaaaaaaga
      961 aaaagtcaaa ggcatcatta cctttgccga agataatgag aatctgcaac atctagaatt
     1021 gtatcgcata gacaatgtat agattcatga gttagctaag acgacagata gacaatatct
     1081 gtcgtctttt gcagtcttga gacaattgcg acaaaattcg ggtcttgaca ttgttttggc
     1141 atttggttta cacttataaa gtattaatag gtgataaata gaacgtagtc atttttaaaa
     1201 ctttatgtat gaaatagtaa taatgcatat taattatgct ttttatttta tgatgaagtg
     1261 gagtttgatg acgatgaaaa cgatgaagac gaccttgatg ggtgccctta tgctgagttt
     1321 gggtatgacg gccagcgtat tggcagcacc ggcgacgacg gccaacgttt ccgtagacag
     1381 cccgtattat ggctatatcg aaaaattgag tgccatgggc tatctcgata ctatgcccaa
     1441 tggggccaaa ccgtacagcc gcatgcagat ggctcaatgg gtcgtccagg ctcaggataa
     1501 ggcacagacc aaaccgatgc cgaaatacct ggcggaccag gtcgatgccc tggcccagta
     1561 cgtagcgccg gaagtggcta ccctgcgcgg ggaaaagaca tatgacccgg tgaaattccg
     1621 ctcggtctcg ctgacggcag cagctcagct ctcggatacg ccgcgccatt cctatagccg
     1681 cactgtcaac gccggctggc agacctttgg cgccaatcgc aatggctata aatacggccg
     1741 tgacggcaat ggcatcttgg aagctgaaat ttccgggaac atcggccatg aaacggctat
     1801 cgccctgcgg ccgcgcttca gctatgacaa ggacaacgac ttcagtgcca gcctggaaga
     1861 aggttatatc aagacccgtg ccggtatctg ggcctttgaa gccggcaaag aagccatgtc
     1921 ctggggacag ggggaaacgg gaaatctggc cttagggaac aacatgcgtc ccttgacgac
     1981 catccaggcg cacttcattg aaccgcagaa agtaggcggc ttcttccgtt tcctcggtca
     2041 ggcagacgtc caccttttct acggtttcct tgaaggcgac cggcgtgatc gtgcggctgc
     2101 ccggggtatg acggattacg acgatgccgg cctcatcggc atccgcgctg acttttcgcc
     2161 gacatcatac ttcaccttcg gcctgtcccg cctgtccatg ctcggcggtg acggcaatgg
     2221 tctggatagc tccgactggg gccattggct ctatggccgc aatgacgatg ccgataagga
     2281 ccgctgggat gacattgccg gcggcgactt ccgcctgagc ctgcctggcg tcaccttcta
     2341 tggcgaactc tacggcgaag accagtcgca ttatatgccg tccaaagtcg cataccgggc
     2401 cggtatctac ctgccgaaac tgactcatga cgggtcctgg gacatgacct tggaaatggc
     2461 cgataccagc gatgcctggt acggtcacca gcgcttcaat aacggctgga cctatcacga
     2521 cgccatcatg ggcgatgcca tgggccgcga tgcccgcaaa tactacggcg ccatccgcca
     2581 ttatttgcct catgaaacat ccatcggcct ctatgcccag cggaccgaaa tggaacgggg
     2641 catgcgcatc catcctacag tcaatgaatt cggcctcacc ggccagacca aacttgccca
     2701 ggacgtctac ttaagcggca tcatcggcta cgccaacgta gaaaacgccg acttcaccat
     2761 tcatacagac cacgacaaat tcgccaccgc caccatccag tggaggtatt agaatacggc
     2821 tctcttgata ggcccttttt ggcccgtcag ggactgaccg gagggaagtg caggacggtt
     2881 gccaaaacgg agagccgtgc tagagttgtc agcgaagctg actgagaggt ttcgcaggcc
     2941 ggcgcccacg accaaccaac ctctccggcc tttcaggcca cctcccctat aaagggaggc
     3001 ataacgacga acgatctacg atgaacgaca ggggccccgg cccccagacg gagaactatc
     3061 atgatagaca ttcactcaca tattttatac gacattgatg atggctctga atcgctggac
     3121 atgtcgctgc agatgctgcg catgtccgtc gattccgggg tcaccgatat tattgcgacg
     3181 cctcatgtca atcgtcaggg ccatattccg gcctggcaga cgattctcga caaggtagca
     3241 gcccttcagg aagaagcgga taaggctcag attcctatcc gtatctatac gggcgcggaa
     3301 gtggaactga acggggacac gttgcgcttt atgaaggaaa atcacgacga ttattgtctg
     3361 gctggcagcc gttatgtcat gttggaactg accgaccagt cccagccgga tatcgtcgaa
     3421 aagatgattt atgagctgca aatgcgcggg ttcctgccac ttctggccca cgtagaacgc
     3481 tatgagcgcc tcatggagcg cccggaaaca cttctggcat ggatgaataa gggcgtcctg
     3541 gctcaatgca atacaggcag tttcacgggc tatttcggct cgaaactgca gcagcgcgtc
     3601 cgcgacctct accgcaatgg catgatccac ttcctcggca gcgacgggca tcgcgtggaa
     3661 tctcgcacga cggatatacg cgaagcccac gaagaactgg accgcctggc tgccaaggat
     3721 aagcagcacg tcaatctttg gaacacggcg acgcgcaatg ccgaatgtat tttaaagaac
     3781 cgcgtctttt atcccgactt gccgtcgcac tggcaaaagg agaaaaaagg ctttttccat
     3841 cgtctctttg gttgaatata ggatgtgaat acagatgtta aagaaaagta tactggcact
     3901 ggccgccgtg accgctttgt ggaccgggac ggcctgtgcc gaagagtatt atatgcggcc
     3961 tggcgatgag ctcaacatcg tcgtcaccca gcagcaggac ttgggcaatt cgtcgaccaa
     4021 tcagtcgccc ttcgtcgtcc ggccggacgg caacgtatcc tttcccttag tcggggaaat
     4081 ccacgccgaa ggcatgacgg tctcccagtt tactgacgtc ctgcagcagg gcctggcccg
     4141 gtatatcgtc gatcccgatg tatccgtcaa tatttccaag ttaggccgcg tccgggtcta
     4201 cgtctttggc gaagtcaaga agcccggtgc cgtagaactg gaaaagggcc ataccgtcat
     4261 cgacgccatc ggtgccgccc agggctttac gcgggatacg gcgaagaaga aaattttcct
     4321 catccatcag gaccagccga agtcgctcat tcccattaac ctcaataata tgctcaaaac
     4381 cggcgatatg tcgcagaacg tcaccctccg cgaaggggac atcctttatc tgaccaagaa
     4441 tcatcgcatc gactttgccc gcgatattgc gccgatcttc agcagtattt acatgattac
     4501 cgaagcgaaa gacaacttag ataacaatta gtatttacca tacaggagtt gaacagggtg
     4561 gaagaaataa ccattgattt gaaagacctc tttcaagttg tcatggccaa caagaagaaa
     4621 atcgcggcta ttacggcggc cttcatgatt gccggcgggg cctatctgct cgtcgcgccg
     4681 ccggtgtacc agtcgacgtc gctgctgcgc atcaagcagg ataagggcct gggcgattcg
     4741 attttgtcca aagtatccgg cggcaatgcc atggatgaca agcagcgcat gatgaccgac
     4801 gcagaaatcc tcaagagccg caacgtcgtc ctgccggtca tcgcccagac agaagaacgc
     4861 gatgaaaacg ggaaattgcc cgactatgac ggctatgtca agagtcatat cattaccaaa
     4921 ccgtataaag atacagaaat tctggaagtc gacgtcaccg gcaagtcgcc ggaacaggcc
     4981 cagaaagcca ataacctcat catcaagggc tttatgaacc gcctgaccga actgagccat
     5041 gacgaacaga agcggacccg cgagttcctg gaaaagaggc tggccgcgtc caaagacgaa
     5101 ttgagtcagg ccgaagataa gctgcagcag tatcaggccg ccaacaagat gtactctacc
     5161 gacgaccaga tgaagcagct tacggataag ctgaacattg tcgacaaggc taaagccgaa
     5221 aaccagctca acctggaaac ggcccaggcc ggcctgagca gcgtcaatga acagctcagc
     5281 tcagcgggtg tatccatcgc cgacagcccg gctatccagc agtataagac ccagctggct
     5341 cagctggaag cgaccaaagc cagttacgtc ggcaaatata cggatgaaca tccgaagatg
     5401 caggaaatca acaaccagat caactccacc cgcgattctc tgaatcagga aatcagcaaa
     5461 atcgtcgccc agcaggcgcc gtccagcaat actgtccagc agggcttatt ggccgataaa
     5521 ttcaagaacg aagcggccat tgctgttgcc cagagcaaaa agacggccct ggaccagatg
     5581 gattcggaaa atgacgccat catcgcctcc ctaccggaaa aggaacaggg cttcgtccgc
     5641 gtcaagcgcg atgccgacgt caacaacgaa atctatgtca tgttggctaa acgcctggaa
     5701 gaagccaagg tcgccgaagt catggtgccg aacgaagtac aggtcgtcga ctgggggaca
     5761 ttgccggaaa aaccgattaa gccgcggaaa gtcctgatta tggacatcat gaccttgtta
     5821 ggtctcatcg tcggcatggg gacggtcatc gtccaatccc tcatgtatcg caagatacgg
     5881 acggccgaag acgtggaaaa agaattgggc ctgcctgtcc tgggcatgat tcccgatacc
     5941 aataccttgc agaaggaacc ggacagcagc catggcttgt ggtcgaaact taggaggaaa
     6001 ttatggcgga aatagatttc atcacccatt atgacagccg gtcgccggta gccgaagcct
     6061 atcgggccat ccggacgaac ttacagtttt ctggtgccgg caagaccttg aagaccatcg
     6121 tctttacgtc ggccatccca aacgaaggga agtcgacgac cgtcgccaac ttggccatta
     6181 ccatcgggca ggacgataaa aaaatcctgc tcatcgactg cgacatgcac aagcccgtca
     6241 tccatcgccg tttcagcctg ctcaaccggg gcctgagcaa ctgctttgcc gaagacttgc
     6301 ccctgaaaga agtcatccag gccgacgtct ttcccaacct cgacatcgtc accagcggtc
     6361 ccatcccgcc gaacccggct gaactcctgg gctcgaagaa gatgaaggcc ctcttgcagg
     6421 aagcagcgga aatgtatgat tacgtcttcc tcgacatgcc gcccgtcctg gccgtcactg
     6481 atgccgtact catgagcagc cagacnnnnn nnnnnagacc gacggtacca tcctcgtcct
     6541 gggcagcggc gacatcagcc cggacgaagg caaacaagcc aaagaactct tagaaaaagt
     6601 ccacgccaac atcctcggcg tcatcttaaa taaagtcccg cagcaccaca agagcggcta
     6661 ctattactac tactattacg acgaaaatca taataaaaaa cggaaaagac gttagtagta
     6721 tggctccccg gataggccct ttttggcccg tcagggactg accggaggga agtgcaggac
     6781 ggttgccaaa acggagagcc gtgctggagc tgtcagcgaa gctgactgag gggttcttac
     6841 agcgaccagt cagctatata tccaactggt caatgtggca tggtttcacg acgtggcatg
     6901 gtttcacgac cggcggcctg cggtctgcga ccggcaaacc aacctctccg gccttttggc
     6961 cacctcccct atgaggggag gcttaacgac gaacgacggg gccaccggcc ccaaactcaa
     7021 cgaaaggaat gaaaaaaggt catgaagacc cgtgtctatc ctacgtcacg taaacttttc
     7081 ggtttactcg gtgacgtgac tatcatcacc ctaacttaca tactcctgac ctatctgatt
     7141 atcccgccga gtgcgtggga agtacatcag gctttatact cggggatgct gccgattgcc
     7201 gttatcttca cggtcatgat gttcagtatc aacggtcttt atacgattga acacaagcgc
     7261 ttcagcgaag tcctgatcag tctggctgtc agtctcttcc tggtcctcat cctggtcatg
     7321 gccttgagtt ttgtcattca tgaattcgct tattcccggg tattgattat ttccagtacg
     7381 gccgtacaat tcgtcctgct cggtttttgg aagcgggccg tccagcgttg ggaacagagc
     7441 cttcatcccg tccgacgcgt catgattatg ggcaacgaag acgaatgtcg tcatgtccat
     7501 taccgcatga gtgcccagcc tcagctcaag ctgaagctcc agcacgtcat catggacccg
     7561 cagaaggatt ggcgtcagga actggaatcc tgcgacatcg acacactcat catctgctcg
     7621 gatatcccta tcacgactaa ggcggatatc gtcaactatt gtacggataa agaaatccag
     7681 ccctacatca ttcccggctc ttacgaaatc ttctgcaacg gtgccatgct gcagaaaatc
     7741 gacgacatcc cggtcttccg gcccaagagc ctgcgtctca gcgtcgaaca gcgggccgtc
     7801 aagcgtaccg tcgacatcat cgtggccggt atggccgcca tctgtgcctt gcccttcgcc
     7861 attcccgtcg ctctggctat taaactcggt gatggcggtc ccgtcctcta cagccaggtc
     7921 cgtgtcggcc gcttcggcaa ggaatataaa gtctataaat tccgttccat gcgcgtcgat
     7981 gcggaaaaat attccggtcc ccagctggcg acggaagacg acccgcgcat taccaaagtc
     8041 ggtaaattca tccgtgccac ccgcctggac gaactgccgc agatcctcaa cgtcctcaac
     8101 ggcgacatga gcctcgtcgg tccccgaccg gaacggcctt tcttcgtcga aaaattcaaa
     8161 aaagaaaacc ccgaatacgc ctatcgccat aatgtcaaac cgggcattac cggtctggcc
     8221 caggtcttcg ccaagtataa caccacgccg tacgataaac tcgtctacga cctgatgtac
     8281 atcgaaaact acagcatctt agaagacttc atcatcatga tccaaaccat caaaatccta
     8341 gtcaccaaga gcgctaccga aggcaaagac gtcagcggta aagacctaga cctgagtaaa
     8401 tttgaacgct aaagcctccg cctcccctga gaaggggagg tggcccgaaa gggccggaag
     8461 ggttcttccg caagacggtt gccaaaacgg agagccgtgc tggaggtggc ccggaagggc
     8521 cggagaggtt cttactattt ttaaaaagga tggattattt atgcctgtca attattctgt
     8581 tttaatgtct gtctactaca aagaacatcc cgattactta cgacaaagca tgcaaagcat
     8641 ttatgaccag actgttccga ccgacgactt tatcctcgtc tgtgacggcc cattaacgca
     8701 agaactggat gctgttattg ctgatatgca gcaacaattt ggctcacgct tacatatttg
     8761 tcggctttta agaaatgggg gtctcggcaa agctttgaac tttggcatcg ggcaatgtaa
     8821 aaatgaactc attgcccgta tggattctga tgatgtcagc tatccaaatc gatgtgaacg
     8881 gcaacttttg gtttttcaaa aacatccaga attcagtatt gtaggtggga ttgttgaaga
     8941 gttttctgat actatccaac ac
//
LOCUS       DAWABZ010000075         8714 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2181_length_8714_cov_3.383185, whole genome shotgun sequence.
ACCESSION   DAWABZ010000075 DAWABZ010000000
VERSION     DAWABZ010000075.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 8714)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8714)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8714
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2181_length_8714_cov_3.383185"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1986 a         2043 c         2488 g         2197 t
ORIGIN      
        1 attaaatgtt gttggaccca ttattgccaa gaacctggac aacattggcg aaatacaaat
       61 tcaaggccat gcagatatta gctttgaaga cgatttcaat ttacagcttg ctgcaaaccg
      121 ggctattgca gtatataaat tcctgcaggt caatgtaggt atagacccgg cccaatactt
      181 gatgtcagca acatcttttg gtaaatataa acctgtaggc cgtgatgccg gcaatgacta
      241 tagctatgca caactgcaag ctgctaatgc caacgaaaca ttaatggcga aaaatagacg
      301 tattgaaatt gttttatttt ataagaatca taaaaaccaa taattttcac ataaaacaat
      361 tcaaaagagg ctgtccttgt acgacagcct cttttgaatt tctgtataga gatttgagag
      421 agaaagcgga tttagatttc tacgttgaag tgtttgagaa tcattttttc ggcttcggcg
      481 ttccacagtc ctttgtgggc tttgcagcat ttgtccagtt ccaggaagaa cgggtctttt
      541 tcaccgagtt tggcgaagtc ttcgatggcc gtcatgggca tatcgaactg ggtgtaggcc
      601 agttttttgc cgcccgggat tttcggcatg ttgagcgttg tttccgcgat gctgtcgata
      661 ccgccgacgt gggtgatcat gacggccggg tcaatgatgc ctttggctgc cagtgtattg
      721 gcttcgatga ggtcgtcgtt gttgccgcct gtcgtaccca tgatatgggt gctggtgtaa
      781 tggcagtcat agaggttgat gtcggcgctg aagtttttgt ccgtcgggcc ggagaaaaaa
      841 ttcatgcagc cgtcgaaggc caggagtttg ttgcccagtt cggcgacggc gcggatcggc
      901 gtgtagacga agacgtcgtc gtagccgtgg ccgtcggtga gcttcatcaa gtcatcatag
      961 gccgtatcca tgttggacgt attaacgtag atgagttcga cgccgtgctg agctgcgtat
     1021 tccggcgata cgacttcttt ggcccgggcg atgcgggcat cgttgatatc ggtgacgacg
     1081 atgcgcttcg gcttgttttc aaaatgaatg ccgtagctga tggcaccgag acccatgggg
     1141 ccgcagccgc cgaggatgat gatattgccg ccttctttgg tgcccatggc gtggttgtaa
     1201 ttgtgtttat tcgtatgata gttggcatgg tagccgccga tgatgcagct catgggttca
     1261 cctagggacg cttcaaagaa gctttcgccg tcgtaaggca tgagagcgcc cagttccatg
     1321 acttcgtgcg ggaagatgca gtatgtgcag tcgccgccga agaattcata agagtacccc
     1381 ggggaagcca ggctgccttt gtagttcagg gccggctgct gggcgaagcg catgcccggt
     1441 ttgaactgat cctgccattt cttgccgact ttgacgatga cgccggcgaa ttcgtggcct
     1501 gtgatgattg gatgtgtgtc catattctgg ggagcgcgtt tatgtttctt gccctgttcg
     1561 accagtttat acgtcgacat gcagatgctg tcggtaatga ttttggctaa gatttcgtcg
     1621 tctttgattt ccggaagttc aaattcttcc aggcgtaaat cgcgggtacc gtacattctg
     1681 actgcttttg ttttcatgat agagtcctcc taaatgattg ttctgtaata atatatgtat
     1741 taaaagatgg atgattattc tgcttccata ttggcgaagg tattgtaaat gtcatcgacg
     1801 gaactcttga ggatttcgtc gacttcgtct tcatcggaac aggtgatggc aatgttgctc
     1861 aggatgtcga tgtgttcatc gccgacgccg gcgatgccga tgactaattt gactgtttcg
     1921 ccgccccagt ccgtaccttg agggaaggtc atgataacca agcctgattt cttgacttca
     1981 catttgcctt cttcgctgcc atgagggata gcaacggcgt tgccgatggc tgtattgaag
     2041 accttttctt tgtccagcat ggcctgggta tatttttcgg tcgtatagcc gtcgtcatag
     2101 aaaatcttgc cgatttcctt gatgatgtcc tctttggctt tgggctgaca gttgaggatg
     2161 atatttttct tctgtaaaat cgtgttcatg gtacctatct cctttattca ctttgacggg
     2221 cagcggccaa ttcatcgacg agcatgccgt attccggggc tgccataaaa ttagtaattg
     2281 taatcagacg ggcctgtggg gcgctggcgc gggcccggtc ggccaaatcc tggtgacaga
     2341 cgacaagctg ggcatctgcc gggatttcag aaacggaagc gtggctgaca gtgatatcgg
     2401 ttaggccggc tttcttgaat ttcttctgca agacggcggc tcccatggca ctggaaccca
     2461 tgccggcatc gcaggcgaag acgacgttag tgatgtgttt caaatcaact tgggccggag
     2521 cggcagctgc cgggaccgtg cctttcgatt cggccttcat gtcctgcatc ttctgctgag
     2581 cctcttccag ggattcgccg gatttagcag cggacatctt gataatcggc gaggcgatga
     2641 cgaaggatac gctagcggcg atgacgatgc tcagaatgac cgagagggtc gagcctttcg
     2701 gggccatggc caggtaggaa atgaggctgc ccggagccgg cgggccggaa aggccgaggt
     2761 cgaagatcat gttatagaac agggcggcta cactgccgct gatagtagcc agcaggaggg
     2821 ccgggttcat gaggatgtac gggaaggaaa tttcgtggat gccgccgagg aggtggatga
     2881 tcagagcacc gggagcggac tggcgggttg tattgtcttt ggagaagaac cagtaggcca
     2941 ggaggacgcc tgtgccggga ccgggattag tttcaatcat ataaaagatg gacttgcccg
     3001 tttcagcgac ctgctccgca ccgaggggag tgaagatgcc atggttgatg gcgttgttca
     3061 ggaacaggac tttagccggt tcgacgaaga cgccgatgag cgggaaaatg ccggcctgga
     3121 gcaggatttg gacgccggct gtcaggaagg acaagatgat gcccatgacc ggaccgatga
     3181 ggtagaagcc gatgatggcc atgaccatgc ccaggatacc gacggagaag ttgttgacga
     3241 gcatttcaaa gcccggcttg atgcggccat cgacagcagc atcgaatttc ttgatgacca
     3301 ggccagccag gggacccatg accatggcac ccatgaacat ggtgtatgtc gtaccgcaga
     3361 tgacgccgac tgtggcgatg gcacccatga tggcgccgcg caggccgccg acgattttgc
     3421 cgccctggaa ggcgatgagc aggggcagga cataggtcag gaaggggccg accattttgg
     3481 ccagctgttc attgggcagc cagccgctgg gaatgaataa ggccgtgatc aggccccaag
     3541 cgatgaaggc gccgatgttc ggcatgacca taccgctgag gaaacggccg aaggattgga
     3601 cttttgcttt catggtgatt cctcctaaaa tgcttggaat gaatacgcta aacatgagca
     3661 gtacctgaca ctccggattg cactgcgctg ctctcttgat ggctacagta taacttgtgt
     3721 acatatcaaa agcaattaaa agtaatttaa gtttgtcctt ttgcaagatg acaggacttg
     3781 agaaatgagg cttttacagg cttcatccct actagccata acgcagtttg tggtataatg
     3841 aaggtaaaaa atcacgaaga ggaggtgttg acatgggggc gaaagccgtg atcatcgatc
     3901 acgaaccact gatggcggcg cagttaaaaa aaatgttaca gaaggggaaa acagctatcg
     3961 aggtgaaagc gatatgctat gaaggggagc aggccatggc ctgtctggca gctcatcagc
     4021 cggatatcgt ctttctgacc ctggaaatgc cgcgggtcag cggcatgact attgccaagc
     4081 agatcgaagc ggaaaaagag cctcatccgg ccattgtctt tgtcacgggc cagcgcgatt
     4141 tcatttctca gacgctgcgg ctcaatgtcc tcgattatct ggtcaagccg gttacggaag
     4201 gtgaagtccg gcgggttgtc gaaaagttct gtgccgtcta tacaaaaaaa gaagcccctt
     4261 cacaggaagg agcttctctg acgaccgccg acggcggcga acggcctagt tatgcccggc
     4321 gcttttccgt cgatgaaggt gataagataa aactcatcgc aacggaagat atccgcctgg
     4381 tctatgcgga aaagcgcaag gtctatctgg ttactctctc aggccggact tatcccagcc
     4441 atctcagtct ggtccagttt gaaaagcgtc tgcctgaaga catctttttc cgctgtcacc
     4501 gcaattacat cgtcaatatc gatgaagtcc agcaaattga accgtggttc aaccatcaat
     4561 acgtcctcat cctgaaaggg atggaagacc agcccgtgcc catcggccga tcgtatgtca
     4621 agaagctccg gcagtatgtc gacttatagg cccagggcgg actcgaaata ggccttgaga
     4681 atccgctgca agtggcagcg gattttttct gggtcgtggc cgaggatggc ctggtaaaat
     4741 gcgtcatcgc tgaccaggga actgctgatg aagcccagta cgtccgtatg gacctggcgg
     4801 tattcgtctt cgggcatgat gagcagcagc actgtcgcga tgcctttgaa gtagtcatcc
     4861 ttaaaggccg gaccgtcggg aacacaggta tagaagcggg attggtggac agtctgggtg
     4921 cggcagtgga gcagggcaaa gccctggtca ggaaaaatct ggctgttcag ccgttcgcgg
     4981 cggatgatgg cgtcgaataa gagggctgta tcgtcttcgt tctccgtgac tgcactggct
     5041 aaggtatgga cgacttgggc aaatgtagcc tgtgccggga cggtgaattg ctgataatgg
     5101 cgcaggaggt cggccatttc ccgggccaga aggtgcgtcc ggtccagggc tgataaaaag
     5161 gtatctcctg aaggcgtgac caggccgggg tctttccgga tatggaaata agtatcgatt
     5221 tttcccagaa tatgctgaag ttcggatccc gtaatgaggg gcgatgtgcg caggacgtcg
     5281 gctccaggaa tctggagggg cagggtgctg acgaaaaagt cggtctggtt caatacgctt
     5341 tctgtcaggt cgtctttacc atacgtatgg agctcgacat ggctaggaag tttgttctgc
     5401 aggcgcgtca tcatcaggcg ggccaggccg aagccgctgg cacagacgat gccgatgacg
     5461 actttgcggg taaaggcttt tttccccttg agccgttcca gggccgcgcc gaagtggatg
     5521 gtcagatagc cgatttcttc ttccgaaacg gtcgtatcca aggcacgggc cagaacgtca
     5581 gcagcgcggc gggctttttg gaagatagag gcgtattcct gctggatgtc gtccagcagg
     5641 gggttgatga tattcaagtg atgttttagg cgtacaatgg tcggctgcaa atggacgaaa
     5701 aggccgcgga tgaattcgtc gtcacagcgc aggtcatagg catattgtgg gtcgaaggca
     5761 tcaatcatcg catcgataat gccgaggaga gccgattcat ccagtccgtc aggtccctgc
     5821 caggagctgc cgctatagcg gattttggcg cctttgatgt gcaacagcag gtatgacact
     5881 tcgatggccg gcatggacag gtgaaactcg ttttccatgc ctttcaggat gcggcgcgcc
     5941 agggcgctgt cttcgtcgtc tggaacggat ggtgggactg agggttcttc ctggagctgg
     6001 ccgctggcta cgcgttcgat ggcgatggcg atatggatga ccaggccgat tttggcagca
     6061 tcagtgaagt gggtgatcct ttcttcatgg agctccttga ggacggcata gacccgctgg
     6121 atcgtgtggc tgttgagcaa tttataaata cctttgtcgg tgacgttcat gacagcttga
     6181 gaaagggctt cgtggtctgc gtgaagttct ttgacatctt cggctgacgt cgtttcactg
     6241 atgaagcggc gcatggcttc acgatagtct ttttcgctgc ctgtgaggac gacgccatag
     6301 ccctgcttct tgaggacggc taagttattc ttttgcagcc acggtgtcag tgattccagg
     6361 tccgacgcgg ccgtggcttc actgacgccg aggagctggc tgtaatagaa aagcttcttc
     6421 ggcgtccggt cgcgcaggat ttcaaagagc agataacggc ggcggttgtg tttatccgtg
     6481 aaatccatgg ccgaagccgt ctgtaagttg gcagtcagtt tttctttccc gtctgccggc
     6541 ccgatgagtt taaggccggc ttttttcttg cgctgtaatt gcaggccgta ggaagccagg
     6601 gcaccggccg tcccgtcgag ttcccgttgg actgtccgtt tgctgatgcc caggtcatca
     6661 gccagggcct gctcagatac aaaatcctct tcggacagca ggcgctgtaa aatctgttgc
     6721 atacgagggg taaaatccat gctatcacct ccatcgtcca tggctttcct gtatgtcttt
     6781 gctttttagt atagcccagg ctataggccg gtgcaattca aagtatttta attttgtcat
     6841 cgtcgcagat gacagcattt tgataatgca tagattgcag gatgagggtg aaaaaatgcc
     6901 tggttgttat agaataatgg caagccatat gatataattc aaagtaaagc cttgtaaaga
     6961 atatgaaaga atcgtgtaaa ttttgataaa ggcttattct tatcttttcg ttaaggagat
     7021 cgatgattat ggcattccac ttaaagccca aggaagaaaa attcttttcc ctattggacc
     7081 agcatgcgac gttgagctat gaagcggctg aattactgag ccaggctatt aacggcgaag
     7141 tcgcaaaaga aacggcgctg gaaaaaatcg acgctctggc cgaccaggcc gatgcgctgg
     7201 tcatggaaac gatgaagcgc ctccagaaga ccttcattac gcctatggac cgtgaagata
     7261 tccagcagct gatcgatcag ctcgatgcag ctgtcgacaa catcagtgaa atcatggata
     7321 aaatgtgtat gtaccaagtc ggcgaagcta cggatggcgc taagcagatg gcgaccatta
     7381 cggcgaaagc catgaaagag atccgcaaat ccatcggcta catgaaggac ctcaagaaga
     7441 attacctcaa agtcgaagcg cggagcaaga aagtcttgaa attggaacag gactgcgatg
     7501 atttgtacca tgaagaaatg gcgaacttgt tcaagaactg caaggacccc attgaaatca
     7561 tcaagtggaa ggaaatcctc cagtctgtcg aagacgtcac cgatgattgt gaagatttag
     7621 tcgttacgtt cagaagggtt gtattgaagt atgcctgagc attggttaat ttgggccatc
     7681 gttttgctgg ccgttctctt tgattatatt aatggcttcc acgatacggc caatgccatc
     7741 gcgacgggcg tatcgacgcg ggccattaca ccgcgccacg ccatcatgat ggcagcgtgc
     7801 ctcaatttcg ccggggccat gatcagcacc ggcgtcgcta agaccattgg cggcgacctg
     7861 gtcctcaatc cggctgaaat cagcttggaa gtcatcgtag ccgccctcat cggctcgatt
     7921 atctggaatg tccttacctg gtattaccgc ataccgagca gttcgtccca ttccctgatc
     7981 ggtggtgtca tcggcgccgt ctgcatcagc gtcggaccgg cagccctcga tatgggcggt
     8041 atcggcaaaa tcatcttgtc tctgatcctg tcgcctgtcg tcgccttggc taccgggtat
     8101 ttcgtcatga tcggtatcct ctggattgtc cgcaggtatt cgccgctggc cctgaaccgg
     8161 cagttccgca agatgcagct cttgtcggcc agcctgatgg ctttttctca cggttccaat
     8221 gatgcccaga aggccatggg gatcatcgcc ctggctctct tgtccggcgg ttatattgaa
     8281 actatggaaa tcccgatttg ggtcaagatg gcctgcgcta cgtccatggc tttagggact
     8341 tcggccggcg gctggcgtat catctctacg atggggacga agatcttcaa gatggaatcg
     8401 atcaatggct ttgcggcgga tttgaactcg tctatcgtca tctttacggc gactttcctc
     8461 catctgcccg tcagtacgac ccacgtcgta tcgggctcca tcatgggcat cggttcagcc
     8521 gaacgcgtcc gcgccgtcca ttggggcgtc gcccgcagta tgctcgtcgc ctgggtcgtc
     8581 accatcccca tcagtgccct tatgtcggca atcattttca aaattatcga ttttctttaa
     8641 gtgaaattag tttgtagtac ggagtttgta gtttgtagaa gctggtttta aattttttac
     8701 catttgctgc aaac
//
LOCUS       DAWABZ010000076         8529 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2237_length_8529_cov_3.125443, whole genome shotgun sequence.
ACCESSION   DAWABZ010000076 DAWABZ010000000
VERSION     DAWABZ010000076.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 8529)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8529)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8529
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2237_length_8529_cov_3.125443"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    423..432
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5919..5928
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1659 a         2448 c         2726 g         1676 t
ORIGIN      
        1 ctttggcgac gatgtaggga atgccggctt ccatgatgac ttctttggca tagacgacgt
       61 tgacgaaacc gacagggacg ccgatgatca gggccggccg catcttcccg gcatcgatga
      121 gttcccgcag gcgcaggagg gctgtcgggg cattgccgac ggcgacgatg accggccctt
      181 cgagggctgc ggctttatcc atggaagcct gggcccgcgt cgtcccctgc gctttagcag
      241 cggcagctac gtcttcatcg gccatgaagc agactttgcg gcagcccagt ttttttagac
      301 ccggctggct gatacccatg caggccatgt tggtatcggt cacgatggtg cagccgcctt
      361 tcagggccgt caaggcctga tcgacggcgc cagggctgaa acgcaggttg cggacatagt
      421 cannnnnnnn nngtcaaagt cggcactggt gtggacgacg cgcttgatga tatccttttc
      481 gtctggcggc aggatgatgt cgcctaattc gtcttcaatg atttccatgc tgcgccgttc
      541 gatatcggct ggtaaaatgt gttcaatttt catgagttcc ttcctttcga tggatgcagg
      601 ctgcgacgaa gcgggcggcc cagtcccttt tccccaggaa atggatgtgg ggatagccgc
      661 cgatgaagtg gtcgttcgcc atgatgcagg gccaatggtg ctggctcccg gccttataag
      721 ccgtaaaatc atggccgttg tccgtgctgt ctgaatagtg gaattcgtgg gccgtcacgg
      781 tgtcgccagc gcagcagagc aggttgtctt tttcagcctt cagggtgacg taaccgaaac
      841 gcttcaggga cgaggtcatg aaggtttcgc caggtacggc gccgacccag ggccacaggc
      901 ggtcctggct gtcgcgaaag gcctgatgga gatacatgaa tcccccacat tcggcgatgc
      961 acggcagccg ggcgtccaag gcacggcgaa gggattcacg catcgacgcg ttctggctca
     1021 aggcctgggc atacagttcg ggatagccgc cgccgagata gagaccgtca caaggcggca
     1081 gttccccgtc gtggagggga ctgaaaaaga ccaggtccgc gccggcctgc tgcaggacct
     1141 ggaggctgtc ttcataatag aaacagaagg cttcgtcccg ggcgatggcc aggacggccc
     1201 ggccaggatc gggatgaaag gccggactgc cagacccggt caaaggcgct gcctggcggg
     1261 cacaagccag gagttcatca atggcgatag tcttttcggc ctgggcggcc aaagcggcca
     1321 ttttcccttg gaggtcagta atttcatcgg ctgtgaccag tcccagatgg cggctggaaa
     1381 aagtgcagtc cggcatatcc gggaagcagc ccagggcttt caggcccgat gccttttccc
     1441 aggccccttt gaaatgctca tagaccatgg gcttgatgcg gttgacgatg aagccgacga
     1501 cgtggctgtc agcccggaaa gcagccattc cctgcaagac ggctgccagc gacaggccgg
     1561 caccgcggcc gtcgacgacg aggacgaccg gcgtatctgt cgctgcggcc aattcatagg
     1621 cgctggcctc cgaacccgtg ccgacgccgt cgtagtagcc catggcccct tccaaaatgg
     1681 ctacatcagc cgcagcacca tggcacagca gcgtctcccg ggcagctgcg gcgcccggct
     1741 cgtggcggcc gaggagaaag aggtccagat tatacgacgg cgtatccagg acccggcggt
     1801 ggaacatggg gtcgatataa tcaggaccgc tcttgaaagc agccggcgac aggccgcggt
     1861 ccttgagggc ctgtaacaaa gcgcagacca ccgtcgtctt gccgctgccg ctgctgggag
     1921 ctgccaaaag cagccgtgga actggcgttc cttgtataga tgagggcatg aggatctcct
     1981 ttcgtgaatg taaagtcgtt catcgtaggt cgttcatcgt tcatcgaaaa agcaaagacc
     2041 ccggcaggcc gcaggtcact ttgtaggggc cgtcctaagc ctttttggcc cgtcaggtgc
     2101 cgaccagcga gaggcgcaag acggttgcca aaacggacag ctgtggctag gcggcccgcc
     2161 ttcgcagttc gcaacaaggc tcccctgaga aggggagctg gcagcatagc cgactgagga
     2221 gttcttccag caaaggcccc ttgcaaggtg gcagaaacct ttgtgcgaca gtctgggtct
     2281 aagcttcccc tgcgtcctga cggacagctg tcatgatata taccgggttc tgggccatca
     2341 tgagatgatg ctggccgact cgtttgctgc gggccgtgct gacctgggtg atgtccagct
     2401 ggtatccgtt acgggcggcg taataagcgg tgacggcggc cagggtttcc aggctgatgg
     2461 ccgtaatgac catgcggcag gcttcattct tgtcatacag gtcatcgagg atggatgcca
     2521 gattgccttt ggtcccgcca atgaaggcgt aatccggtgc gatgtccaag gttttcaagg
     2581 tctggctggc atcgccggcg acgacggtga cgttagtcag gccgaaatgg ccgatattct
     2641 gctgcaatac agtcagggcc tcttcattga tttcaaaggc atagacgtgg ccaaaaggag
     2701 cctgcaaggc cagttcgacc gtgcacgaac cagtaccggc cccgatgtca cagacgacgg
     2761 cgtctgcttc cggagccagt ttggacagga cgacgctgcg gatttcctgc ttggtcatgg
     2821 gcgctttgcc gcgcaggaag gcttcatcgg gcagaccgtg gacgggccgc acccagggcc
     2881 gggccttttc gttttctacc atcatgacgg ccagggaatc ggcgggacgg tcggtgaaat
     2941 cggcagccgt cccgcggagg atgttttcat tgtcataaga taagtcgcag ccaatggcta
     3001 cggacaccat atccaggccg gcctggcaga gttcccggca caaggcggcc ggtgaatcgg
     3061 acccgcctgt caggacgaag actttccgat gctggcggac ggcggcgacg agggattccc
     3121 ggcggccatg gcggctgacg ataaaggcgt cgttccacgt cgtctgcaag gcggccgcaa
     3181 agtagacgag gctgctgata ccggcgatgc gggtcaccgt gcagccaggg atgtccgtga
     3241 gcaaggcggc caggctgaaa aagccgacgt cgccggacac gaggatggcc atgggccctt
     3301 cctcatcgga caaagaagcg gcaagctgac aaatatcgtc acgccggtac gtcgcgacca
     3361 ggcgcttccc ggaagccgca aaaggcgcca gcatccgctt gtcgccgacg aggatgggcg
     3421 aagcctctat ggccgctttg gcctgccccg tcaacaaagc cggattgccg ggccctgtgc
     3481 cgataatata tacgtgcatg gtatgtctcc tttcatgggc gcccgcaggt cgcagtccgc
     3541 tcggactcag atgccgcggt gcggcaacag tttcaaacgg acaacatcac ttcgagtaac
     3601 cctcgctcgc aaaaatttcc cgaacggggc tatttttcgc gaactgcgtc ttccctattt
     3661 gcctgaatag atatgtgtaa aaagcaacct ctcagtcagc tgcgctgaca gctcccctat
     3721 gaggggagcc ttgctcttaa gcctcccctc ataggccttt tttagcccgt caggtgctga
     3781 ccgtagggaa gcgcagggca gttgccaaaa cggagagctg agctggaggt ggctcaaaga
     3841 gccggagagg ttgctttctt gtcattactc tccccgttcg atgacgatga ccgtggcacc
     3901 tgtttgtctg gcagcttcta atttttcctg aaagccgccg gtgtggccgg agttcttggt
     3961 gacgacgtag tgagcgccgg tctggcggaa catggccgca ttgagttcgg tcgtgaaggg
     4021 gccctgcata caaatcacgt gggccggcag gtagcctaga tccagggccc ggtccaacga
     4081 cggacggacg ggcaggatgc gggcgtagat gcgctgggca taatcgggaa tctgggcaaa
     4141 ggaatccaag tccttactgc ctgtcgtcaa gaagatgggc cccgtcgtgt ggttcaggac
     4201 ttcgacagct tcgccgacgg aatggacggc gatgcagcca acctggtcct catagcggcc
     4261 gcgccggcgc tggatgcggc ggtacgggac gccgacggca gcacaggcct ggcggatatt
     4321 ggccgtcacg actgtcgcat agggatgggt cgcatcgatg acctgagcga tagcctgttc
     4381 ccggagaaag gtttccatct ccccttcgtc catgcggccg gtcacgacgg tcacgaaagg
     4441 gctttcaggc actaacgaag cgccgtaatc ggtggcgacg gacacgtaag cccggatatg
     4501 acagcagtcg gcataatggg ctagtttccg cccttctgtc gtgccgccgg caatccatac
     4561 ggggctcctc ataagtgata tccccgcggt gtgaccagtt tgccatggat gttccgggtc
     4621 tggctgttgc cgatgaagac cgtacagaac atatccaggc gcatcttgcg cagttcgccc
     4681 agggtcagga ccgtcacttc ctggccttcg cggccgatat tgcggacgat gccggccggc
     4741 gtatcggctg gctgatggcg caagatgatg tcacaggcct tttccagata gtctgcccgc
     4801 ttcttgctgg ccggattgta caggcagatg acgaagtcgc cggcagcggc acagtcgagg
     4861 cggttggcga ttttttccca cggcgtcagc aaatcgctga gggaaatgag gcacgtatcg
     4921 gcgaccagcg gcgcaccgag cagggccgca ccggcgctgg cagcggtcac gccgggaatg
     4981 acgacgatgt cgacgtcgtc atcggcagcc acttcgtgca tgatgccggc catgccgtaa
     5041 atcccggcat cgccgctgga cacgatggcc acggtctgcc cctgcttggc ccggtcccgg
     5101 accatgcggc agcgttcgac ttctttggtc atgcccgtcg tgacgaattc tttatcagga
     5161 aaataatgac gtatcaaatc aatataggtg tcgtaaccga cgatgcagtc gcagccatcg
     5221 aggatttccc gggcctgcag ggtcatgccc tgcagggaac ccggtccgat gccgacgaca
     5281 taaatacgat gtttcataac ctttctcctt tcaacatgca aaccggacct ggtagcgttc
     5341 gcaggctacg gctacggtga tgccgcggcg gctctgtttg gtgacgatga gggtcccttg
     5401 atggctggcc aggacggcag cccgttcgca gacgttatcc gtgccgacag tctgtttgac
     5461 gaaatcggac gccgtaaagt cgcctggcac ggcattgagt tcatcggccg taaagaagtg
     5521 cagcggccat tgctgggcct tggcaaaggc caagagaccg gcttcatcat gcttgacgtc
     5581 gatggagctg acgtcggcaa tggcgtcggg cgacaagttg gcccgctgta ggacggcatt
     5641 gacccaataa gcgatgtctt tggccggcgt acccttgcgg cagccgatgc cgatgtggac
     5701 gatgaccggc cgggcgacga cagtcgtgcg gaacacgggc acgtgggctt tggtcgtgat
     5761 caccaggccg gcgtcgccat ggaaggcata gaccaggccc ggcggcagcg gcccctgcag
     5821 ggggaaatcg ctgtacacgc cggcagcgcc cttttccagt atggcagcgg caaaggcctt
     5881 ggcatcgttc agggacgaca ggcgcaggcc ctggcgggnn nnnnnnnncc cattcatcga
     5941 cggcgaacaa ggcgttgaca tcggtcgccg tcgtcacgac ggcctggcct tccagttctg
     6001 cagccagacg gcgggcgatg gcattggcgc cgccgatatg accggacagg atggggatga
     6061 cgaactggcc cttctcgtcg atgaccagga cggccgggtc caggtcttta tggcggataa
     6121 agggcgcaat agtgcggacg gcaatgcctg cggcgccgat gaagacgacc agccgggccg
     6181 cttcaaaagc ccgggcctct tcatcgtgga gattgggtgt aaacggcagg accccttcct
     6241 ggacgacagc gaatttcttc agcgacgcct ggcggacgtc ccagccgcct ccccgcaagc
     6301 aggcagcgac gcggcagccc agggccgtcc cggcctgggt aaaggacagg acgacggctt
     6361 tcatggttcc gtctcctgac cgatgtggtc gccgtactgc tggtcctccg gcgccgcctg
     6421 gcggcagccg tgggtaaagg ccggatgata caaaaggctg cggtcataag ccggccccag
     6481 caccttgccg acgacgatga gagccgtctt ggaaatggca tggcgccggg ccgtttcagc
     6541 cagggtgccg accgtgcagg tataagtctg ttcttctggc catgatgctt tgtagacgat
     6601 ggctgccagc gtatctgccg catagccgcc gcgcaggagt tcggcctgga ggccgtccaa
     6661 gagggatgcg ctgaggaaaa tgaccatcgt cgcctgatga gcggcgtaat cggcgatttt
     6721 ctgcttatcc ggcacgggcg tccgcccttc catgcgggtg atgatgaccg actggctgat
     6781 gcccggcagg gtatattcgg cctggaggga cgcggcagcg gcacagaagg agctgacacc
     6841 gggtacgacg tcataggcga tgcccttctg ggcgaggatg tccatctgct cgcggatggc
     6901 gccatagact gatggatcgc ccgtatgcag gcggacggtc atccagccct ggccctcccc
     6961 ttcttccatg gccgccacga cggcttccaa cgtcatgccg gcgctgtcga ggatgcggca
     7021 gccttctttg gcacaggcca gcagctgtgg gttgaccagg gagccggcgt aaataatctg
     7081 gtccgctgtt tccagtaatt tctggccgcg gacggtaatg aggtcgacag caccgggacc
     7141 ggcgccgaca aagtgtatca tcgtagttcc cccttcgtat gcaatgcaag aaattctttg
     7201 gcccgcgacg tcaggcccag gacgccgtag gcattggaaa agacgacggc cgccgtcggg
     7261 atttcaccat gggtccgctg gtccatatag gcctcgattt tctcacagac tgcggccatg
     7321 accggttcca gcaggttttg ttccttcagc aggcgcacgg cttcatccgt cgtcaggctg
     7381 tcgtaaatct gacgggccag ctgccgtgag gcaccggcaa agacggcttc cagggccagg
     7441 agggtcgtgc ggccgtcggc ataatgggaa tgggtattca tgatgccttg ggcgactttg
     7501 accaatttgc cgatatggcc gatgaccagg acgtcggaaa agcctttgta ggcggcataa
     7561 tcaagggttt cgccgacata gttgctgatg gtcacgcgct ggctgacgtc gacgcccaga
     7621 tggtcgcggc tgaaatcgac gccgtaattg ccgaaaaagg ccaataaatg ggtctggccg
     7681 gcagcgcggc gggaatccat ttccgtatgg atcgtatcga ccagggcctg ttcactcata
     7741 gggtcgacga tgcccgacgt ccccaggacg gacaggccgc cgacgatgcc caggcgcggg
     7801 ttgaaggtct tctgggccag gcgctggccc tcggggatgg acaagttcag gcgcaggccg
     7861 cccgtatagc cgcactgccg gcaagccgct tgcagggctt cggtaatcat gcggcgcggc
     7921 acggggttga tggcccaatc gccgacggca caggccaggc cgggctgggt cacgcggccg
     7981 acgccgatgc cgccgacaat ggacaggccg gacggcgtct tttcgacggt cccgtagacc
     8041 agcatgccgt cagtgatatc cgggtcgtcg ccactgtcct tgcggatggc gcacgtcgcc
     8101 gactggggac cgaaagagac gtcttccggg atgagctgga ggaccgtccc ctgcggcgtc
     8161 tgcaaggata tggcttcgac gcggcgcccg gtcaggacca tgatggccgc cgccttggcc
     8221 gccgcggccg cacaggaccc ggtcgtatag ccgcagcgca ttttcttccc ttctttgacc
     8281 gtgaatagtg acatggtata tttctccttt tatcgttttt cgttcatcgt tcgtcgtgat
     8341 ggatggttat ttttttaaac cttacgtcaa atgatgaacg tatttaacaa aacttcactg
     8401 ataatctgac gtgtcattgc aacacaacga tggccgaacg gccgtcactg gtggtgacga
     8461 tgtcggtccg gacggaaaag acgtcccaaa gccgttccgg tgtgaggacg gcctggggtg
     8521 gtcccgtac
//
LOCUS       DAWABZ010000077         8345 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2313_length_8345_cov_3.408926, whole genome shotgun sequence.
ACCESSION   DAWABZ010000077 DAWABZ010000000
VERSION     DAWABZ010000077.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 8345)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8345)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8345
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2313_length_8345_cov_3.408926"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1647 a         2320 c         2370 g         2008 t
ORIGIN      
        1 tgtacgaggt taaagtaacc agaaccaaaa acgagatgaa tatgaatgct ataacgtaga
       61 ggaccggtag acatatgtcg cgtgtgtatc ggttctttta cgtgtttttt ttacctgact
      121 tgtcccttct ccattgacag aaaccgtccc ctcttctata atatactaaa acgatattct
      181 atagaattcg tttagattta tcaaaggggg agacaattat gtccaacgaa tctcaagacc
      241 gggtccaaga acaagcccgg gaagccatcc gcggcagcca gggccagcat cgcggccgtc
      301 aattcgcctt gtggatgggc gccttgatca tcggcgccat cttaggctgg ctcggcattc
      361 cggcactgaa ccaattattt gatttcattg caacagtttt cacgcgctta ttccagttca
      421 ttgccgtacc gactatcgcc ttagccgtca ttactacttt atcggcgctg ggagccaaaa
      481 aagaaacggg ccgcatcttc gcccatgccg tcacgtacac gctcttgacg accatctgtg
      541 ccgccgccat cggcctggca ctctacctct ggattgcccc gggcaacctg ccggtagacc
      601 tcatcaattc cggcgtttcc aatgtcccgc agaaattgga aaaagtcacc tattacgacc
      661 acttcctgtc ggtcatcccg aataacatgc tccagccgtt cttgtcgggc aacgtcctct
      721 cggtcatgct catcgctgct gctgtcggcc tggccctggc cttcatgcct aagacagaaa
      781 accgcgccgt cctcctgaaa ggcctcctgg gcttacagga agtcctgttc accctcatcc
      841 gtgccctgct ctggattttg cctgtcggca tcctggcttt ctcggcacag ctgtcggctc
      901 agattgaagc cggggtcatc gtcggtgccc tgggtaaata tactctggtc atcctcggcg
      961 gcaacctcat ccagttcttc atcgtcctgc cgattttcct ggtcctccgc ggcctcaatc
     1021 cgatttacgt cttccgcaag atggctccgg ccattgccgt cgccctgttc accaagagct
     1081 cggccggtac cctgccggtc accctggctt cggctgaaca gcgcctcaac gtccatcccc
     1141 aggtatcgcg cttcgtcctg ccgatctgca cgaccatcaa catgaacggc tgtgccgcct
     1201 tcatcctggt cacatccctg ttcctcatgc agaacggcgg cttcgacctc tccatgggca
     1261 ccatgattgc ctggctcttc gtcgccgtcc tggctgccat cggcaacgcc ggcgtcccca
     1321 tgggctgcta cttcctgact ttgtcgctca tgtccggcat cggtgcgcct atcggcatca
     1381 tgggtatcat cctgccgatc tacaccatca tcgacatgat cgaaacagcc gaaaacgtct
     1441 ggtccgactc ggccgtctgt gccatgaccg accacgactt agcttctgaa ctcgatgccc
     1501 agccggcacc ggctcagcat taagagcgta aaaaaaccgc tgtcttaaag acggcggttt
     1561 tttttagttg ttaggggcta gcataactgt tagccgcgaa tggtagcttc tttcatggct
     1621 ttgacgtagt catagatgtg cggagccgca tcgtcgccgt atttagcgat gattttgaca
     1681 atggcactgc cgacgatgac gccgtcagcg acgcggccga ttttttcggc ctgttccgtc
     1741 gtgttgatgc cgaaaccgac ggcacacggt acgtctgtga cttctttagc cgtatcgatg
     1801 atttccttga ggtccgtctt gatttccgtc cgcgtccccg tgacgcccag ggaggaaacg
     1861 atgtacagga agccttcgcc gactttagcg attttctgga tacggtcttt cgacgtcggc
     1921 gcgatcatgg agatgatgtc gatgccatgg gccttagcgg cagcggcaca ttcatcgcgt
     1981 tcttcaaagg gcatatcggg gatgatgatg ccgtccatgc cggtttcctg gcagcgggca
     2041 aagaattttt cagcgccgta atggaagacg ggattgagat aggtcaaaaa gaccagggga
     2101 atctgcgttt ccttgcggac ccgttcgacc agggcaaaga cgtcgtcaat gcgcgtccct
     2161 gctttcaaag cccgcaggtc cgcttcctgg atgacgatgc cttcggcgat gggatcggaa
     2221 aaagggatgc cgatttcgat gaggtccgct ccggcctttt ccatggtcat gatatattca
     2281 tacgtcttgt ccagcgacgg gtcgccggct gtcaagaatc cgataaaagc tttgccgttc
     2341 ttaaaggcgt tgccaatctt actcatgtaa gtccctccct ttatatcttg caatggcggc
     2401 gacgtcttta tcaccgcggc cggacaagca gacgatgatg atgtcgtcct tgctcatctt
     2461 cggtgctatc tgcatggctt cgtagacggc gtgggcgctt tcgatagcgc agatgatgcc
     2521 ttccgtccgg gccaggtatt caaaggcttc gacggcttcg tcgtccttga ccgggatgta
     2581 ctgagcacgg ccggtctgat ggagataagc gtgttccggc ccgatgcccg gatagtcgag
     2641 gccagccgaa atggaataga cttcgtcaat ctgaccgtct tcattctggc agaaaatgga
     2701 cttcatgccg tggaaaatgc cgacttcccc tttggccata gtagcggcat gcatcttggt
     2761 gtcgatgccc ttgccagcag cttcgcagcc gatgagcttg acgtctttat cgccgatgaa
     2821 gtggtagaac atccccatgg cattgctgcc gccgccgaca caggccagga cagccgaggg
     2881 cagtttccct tcccgttcga ggatctgctg acgcgcttcc ttgctgatga cggactggaa
     2941 gtcgcggacc atcatcggga agggatgagg acccatggtc gagccgatga cgtagtgcgt
     3001 atcgtcgacg cggctgaccc attcgcgcat ggcttcgttg acggcgtcct tgaggacccg
     3061 cgagcccgac gtgacggcgt ggaccttggc gcccaggagt tccatacggt agacgttcag
     3121 ggcctggcgg atcgtatctt cttcgcccat gaatacttca cattccaggc ctaagaaagc
     3181 cgctgccgtc gccgtagcga cgccgtgctg gccggcgccg gtttcggcga tgacccgcgt
     3241 cttgcccatc ttcttggcca gcaggcactg gcccagggcg ttgttgatct tatgggcgcc
     3301 tgtatggttc aggtcttcgc gcttcaagta aaccttggcg ccgcccaaat ctttggtcat
     3361 ccgttcggca tagtagagca gggacggacg gttggcataa ttgtcaagca agtctttcaa
     3421 ttcagcctgg aaatcgggat cactcttata atggctgtac gctttttcca agcgctggat
     3481 ttcattcatc aatgtttccg gtatgtattg accgccgaag gggccaaatc ttcctgtact
     3541 catgtagtta cctcctgtaa gcacgtactt gtgccatgaa tcgtttcatt ttttctttgt
     3601 ctttcacgcc gtctgtctca atgccgctgc tcacatccag ggcaaagggt tggagcgctg
     3661 ccgcctgggc ggcattgtcg ggattgaggc cgccggccag aaagtacggt ctgtctatat
     3721 gggccagcag ggcccagtcg aaacagtccc cggtcccgcc ggcgccgtgg tcgagcagga
     3781 tgtagtcggc ccggctgcac cgggcctgcc ggacatcggc ttcgctggcg atggaaaagg
     3841 ctttgatgac ccgtaccttc aaggtctgct ggacagcttc gatcatcgcg tcgtcttcgc
     3901 atccgtggag ctggacgatc tggatggtcc cggccgtcac cagctgccct atgtgtttca
     3961 gactgtcatc gacgaagacg ccgacggccg ggatagacgg gtccagggcc ttgcgcaagg
     4021 ctgcggcctg gtcgtcgctg acgcggcgct tcttgccggc aaagacgaaa ccggcataat
     4081 cgggccggag ttcattggcc caggcaatat cacagggccg cttgaggccg cagaacttga
     4141 ccttaaccac gatagcctcc ggccagctcg tcgagcatgg ctttcttatc gggactgcgc
     4201 atgaaggttt cgccgatgag gacggcgtcg acgccgtggt cgcgcaggac cttcgtgtgg
     4261 tctgccgtct tgatgccgct ttcggcgacg aagaggatgt ccttcggtac cagcggccgc
     4321 aggcggcagc tcgtatcgag ggataccgtg aagtccttga ggttgcggtt attgacaccg
     4381 atgatgcggg caccgatggc gatggcccgg cgcacttcgt cttcatcgtg ggcttcgacc
     4441 agggccgaca ggcccagaga atcggccagc tggaagaagg tccgcagccg gtcgtcgtcg
     4501 aggacggcgc agatgaggag cacagccgac gcgcccaagg cccgggcttc gtaaatctgg
     4561 tattcgtcga tgatgaaatc cttgcgcagg accggcgtat cgacggcagc ggcgatttcc
     4621 tgcaaatact gcgggctgcc caggaagtaa tcccgttcag tcaggacgga aatggctgcg
     4681 gcgccggcct gttcatattc tttggcaatg gcgacgtaag ggaaatcggg agcgatgagg
     4741 cccttcgacg gcgaggcctt cttgacttca cagatgaaag acaagcccgg cttcttcagg
     4801 ttcctttcaa aggggaatcc ttctttcttc ggagctgcca gggcccgctg cttcatgact
     4861 tccggcggga cctgctgttt atccgtgacc agccgcttgg caacggctgc cgtaagatca
     4921 tctaatatca tagggcacct tctcactcgt cgctgacggc aatgcatttt tccagggctg
     4981 ccgtcgcctt gccgctgtcg atgaggctgg ctgcgagctg gatgccgtcg gcgatggaat
     5041 cggctttgcc ggcgacaaag aaggctgcac cgctgttcat gaggacggca ttgcgtttcg
     5101 tcccctggat tttcccggac aggacatcac gggtaatctg ggcgttgact tccggcgtcc
     5161 cgccgacgag gtcttctttc gtccccggcg tgaggccgaa gtcttccggt ttgatgaccg
     5221 atgtccggta ataaccgtcg cggagttcac agatcgtcgt cgggccgttc ggcgagattt
     5281 cgtcgagttt ttcctgaccg taagcgacga ggccccgttt gacgccgagg gaggccagga
     5341 ccttggccag cggttcgacg agatagccgt catagacgcc gaggaggaac atttccggct
     5401 tggccgggtt ggtcagaggg ccgaggatgt tgaagaccgt ccggaagccc agttccttgc
     5461 ggatagggcc gacgtatttc atagcagcgt ggtatttctg ggcaaagagg aagcagaaat
     5521 tcgtttcttc gagcatcttc aaggccagct gcgggtcctg ctggatgttg atgcccagcg
     5581 cttccaggca gtccgccgta ccgcacaggg atgaagcggc ccggttgccg tgtttggcga
     5641 ccttgacgcc gcctgcggcc aggaccatgg ccgacgtcgt ggaaatattg aaagaatggg
     5701 cgccgtcacc gccggtgccg acgatttcca ggacttccat gcccggatgg ggaacgggcg
     5761 tcgccaggga gcgcatggct tcggcacagc cggaaatttc atcgatcgtt tcggctttgg
     5821 tgctcttcgt cgaaagggca gccaggaaag cggcgttctg agtcggcgtc gtttcgcctt
     5881 tcatgatttc catcatgacc tgatgggctt cgtcgtaagt caaatcgcct ttattgacaa
     5941 tcttgataat cgcttcttta atcatcgtaa gtacctccat tcaaagagtc atctatatta
     6001 cttataatgt aaggaaattt tctaccatca tcggcccctg gggcgtcaaa atcgattcgg
     6061 gatggaactg caggccgtag acgggatact gcttgtgacc gacggccatg atttcgccgt
     6121 cttccgtatg ggccaggact tccaattctt cgggcagcgt atccgggtcg agggccaggg
     6181 aatgataacg gccgacgtcg acctgttccg gcagcccttt aaacaacggg ctgtccgttt
     6241 tcagcgtcgc tacggactgt ttgccgtgca tgagctgctt ggcataggta atggttccgc
     6301 cgtagacttc gcagatggcc tgatggccta agcagacgcc caggatgggg accttgccgg
     6361 cgaagtagct gatggcttct tcgcagacac cggcatcggc gggacggccc ggaccgggtg
     6421 aaatgatgag gtgatccgga tgcatggctt caatatcctg gacggtcttt tcatcgttgc
     6481 ggatgaccgt gatatccggg tcgaactgac cgaggagctg gtagagatta taagaaaaac
     6541 tgtcgtaatt atcaatgaga actatcattt gtcgattcct ccattagcct gctgtaatgc
     6601 aatgacgacg gctttggcct tgttcagaca ttcctgatgt tcctgttcgg ggatgctgtc
     6661 ggcgacgatg cccgcaccgg agcaggcata gacgtgattg cctttcttgt aagccaggcg
     6721 gatgccgatg cagcagtcca tattgccggt aaagtcgatg tagccgatgg cgctgccgta
     6781 gacgccgcgt ttgtggcctt ctagttcgtc gatgatttca caggcccgga tcttcggcgc
     6841 ccccgacagg gtgcccgccg gcaggaccga agcaatggca tcgacagccg tcttgtcctt
     6901 gcgtatcgta ctgtggacag tcgagccgat gtgcatgatg tgggagaagc gcaggacgtc
     6961 gagatagcgt tcgacgtgga cgctgccgaa ttcgctgatg cggcccaggt cattgcggcc
     7021 agagggaagg tgaagaggtc gccattttcc aggcgggtca gcgtttccgg cgaagaaccg
     7081 gccagttcga tatcgtcacc agagaagaag aacatgtacg gcgacggatt gatggtccgc
     7141 aggacgcggt acgtatcgag gaggctccct tcggcttcgg cgctcaggcg gttggccagg
     7201 acgacctgga aaatatcgcc ttcacggatg tagttcttgc ccttttcgac catctggcaa
     7261 tattcttctt cattgaacaa gggtttgaaa tcgctcttga gctgcagcgg tacgtccggt
     7321 gccttcttgc cttcgcagat gaggctttcc atgcgcttca tttccaggag ggcccggttg
     7381 taattttctt caaccgagtc gaggcggaca ttgacgatga ggtaaatctg ctgtttcagg
     7441 ttatcgaaga cgatgacctt gtcgaacaac atgaggtcga aatcgttgaa gccatcatca
     7501 tcgacggcat tgagcttcag cgacggttcg gcgtatttga tgaaatcata ggcaaagtag
     7561 ccgaccaggc cgcccgtaaa gggaggcagg tccgaaaggc gcggcgcctt attatctgcc
     7621 aggatctggg aaatgagctg ttccggatgg tccgtatgga tctggacttc ggcgccggtc
     7681 ttgatgtgga ccgtgctgtc cttgcagtac aggcccatct gcgggtcata gccgatgaaa
     7741 gtgtagcggc cccagcgctg ggagtcttcc atgctttcca gcaaatagca atgagagctg
     7801 atgttcttga cgatgcgcaa tacttcgatg ggcgtgcgga cgtcggcata tatgctgcgg
     7861 cagatgggca cgaccttata ttcaccttga tatgctttta cctgttccag actgagcgtt
     7921 tccatatgag cctcctaaaa aaagaatccg tccatgacaa tgttacttgt caaggacgga
     7981 ttaagttccg cggtgccacc ttgtttcagg gatcgagccc tactctcgac ggaataccaa
     8041 catattcccg gcaactgacg tatgccaaca cgtcgcagaa tactcagcct tacggccttt
     8101 gactgcgccc tcagcggtcc atttaacgag ctgcgtcggc cggctctcag caccccggct
     8161 ctctgtacgt gcacgtcatc gtttttactt ccgcctcaaa ggtttcatgg acgatgttca
     8221 tttgatggtt gtatgataca ccgcattttt taacctgtca aggagatttt tgtccatatt
     8281 ctaaagacaa tcgttttact ggcaaaaaag aggctgtcgc atgaagacag cctctttttg
     8341 agttt
//
LOCUS       DAWABZ010000078         8182 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2369_length_8182_cov_2.996678, whole genome shotgun sequence.
ACCESSION   DAWABZ010000078 DAWABZ010000000
VERSION     DAWABZ010000078.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8182)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2369_length_8182_cov_2.996678"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1644 a         2413 c         2456 g         1669 t
ORIGIN      
        1 acgcccctca cgccgtgagg cagcaccatt ccggcagggt tacggtacgg tcgaaggccg
       61 ggagccactc gccgcgggta tggtcatagg tccggatgag gatatggtct tcatagaggt
      121 agagggagtt ggtataaatc gtatcttcat cccaatcgct gttgtagaca ttgaggacgc
      181 ggccttcata atagttggcg tcgtccatga aggtcgcatc ggacggcatc gtatgggtat
      241 ggcccgatac ccacaggcgg gcctggtgat ggcgggccag gatggtgcgg atatcccctg
      301 ccggctggac ggaattgcgc ggcgacggca ggctgatgcc gggccggctc gatacggccg
      361 tctgttccag cggcgcgtgg cagaagatga tcgtcggctg ctggcgatgg gcggccaggg
      421 tctggcccag ccaggccagc tgctgatgag acaattcgac agctaagtcc aggtcccgcc
      481 agttattcat ttcctgcagc actctttctt tggcggccat ccggagcaga cgttctttcg
      541 gcgtgaccag gttcttgcag gggtaatgga aatcactgat gatgaccagg cgggaataat
      601 tccgctgaga ttgggctttg gcattggcaa ataacgacgc aaacatggca tttcctcctt
      661 catccatgaa aaaacaggct ctggctgtgc agtttcagta tagccagagc ctgtattttt
      721 tttgcaaaaa tcaagggaaa tgtctgtaaa aggtcatttc ttctggacga cgggatagtc
      781 ctgcagggca tcgcagcctt cttcgccggt ccggatcttg acggcgttgt cgatggccga
      841 cacgaagacc ttgccgtcgc cgtaattgcc cgtatagagc accttcttcg ccgtttcgat
      901 gatggtccgc ggcgacactt tggaaacgac catttcgacg cggaccttgg gcaggagctt
      961 ggccgacact ttggccccgc ggtagaattc agtccgtccc ttttcgatgc cgaatcccag
     1021 ggcgcgggtg atggtcatgc ccgtgatgcc gatggcttcc atggcgtctt tcaaggtttc
     1081 aaagcgcttt tcatcggtaa tgatagtcac gttatacagg gcatggccgc cgaccggcta
     1141 gggactggcc atgaccgaat cggggatagc ctggtccaag gggatttctt gggccccttc
     1201 cggcgcttcc ccggccttca aggcttgcga caaatcctcc aaatcgtcgc tgcggtccag
     1261 ttcgtaagcc ggtaccaggc cgttttcctt gaagtccagg ccgttgattt cttcggctgc
     1321 cgagacgcgc aggcctatcg tcgcctggag gatgcggaaa accagggtca tagcgacgaa
     1381 gacgaacagg gccatagcca cgacgccggc cagctggacc aggaacagac ggatttctcc
     1441 cgtatagaac aggccgtctt ggacgctgaa caggcccgtc aggagggtcc ccaaggcacc
     1501 gcagccgccg tgagcggcaa tagccccgac cgggtcgtcg atgcgcagtt tctggtcgat
     1561 gaattcgatg aggaagaccg tcacgatgcc ggccagggcg ccgatgacaa aggccccagg
     1621 cacgctgacg acatcacagc cggccgtaat agccaccagg gccgccaggc tgccgtcgag
     1681 agtcatggat acgtcgggct tgccatagcg gatccatgtc gtcaccatgg ccgtcgtcgt
     1741 cccggcagcg gcggcgatat tggtcgtaaa gaagatagac gcggctttta ggatggtctg
     1801 ttctcccgtc agggacaggg tctggccgcc attgaagccg aaccagccga accataagat
     1861 gaacatgccc agacaagcca ggggcaggct gtggccggga atggcattga ccgagccatc
     1921 gtcattatat ttgccgatgc gcggccccag ggccttggcc ccggtcaggg cgcagatgcc
     1981 gccggtcagg ctgacgacag aagcgccggc gaagtcatgg aagcccaggg ctgccagcca
     2041 gccgccgccc caggaccagt gcccgacgat gggatagatg agcagactga tgaggatcgt
     2101 aatcagacag gatgacgaaa atttcgtccg ttcagccata gcgccggaca cgatggccgc
     2161 cgtcacggca cagagaacgg tctggaagag caaaaaggcc aaatccggat aaggcgtccc
     2221 ggccatgagg tccatatgct gggcgaagaa atcgggcatg ccgatgaatc cgcctatgtc
     2281 gcggccaaag agcaggctga agccgacgat ccagaaggcc agggtcccga gggaaaagtt
     2341 catgaaattc ttcatgacga tattgccggc attcttgccc cgcgtcaggc cggcttcgag
     2401 catggcgaaa ccgggctgca tgaagaagac caggacggcc gccatgagga tccacaaggt
     2461 atcgaccgat gaataactca ttgcgctccc ccctcttctt cgggataggt caggccccac
     2521 tgctggcgga tggccgtcat gaggtccatg acatctttcg tataagcctg gcacatgccc
     2581 tggccgccgt gggccacggc gtcttccatg tcccggattt cgtactgcaa ggccttggac
     2641 tgactgccgg cctgcaggac atcctgatgg ccgtcggccg tataggtgat ggtcgccgta
     2701 tcggcccggg gatagtcgta gatttcgata tatcccttat cgaaggcggc catgccccgt
     2761 ttcggctgct tggcatggag cgataaggaa agcgtcgcca tctccccttc ccggttctgc
     2821 aagaggatac ctgcctgctc gtcgacaccg gtcggtgcca ggcggacctg ggacgcgatc
     2881 tggtcgggct gacagctcat gaaatagcgg acgaaggaca gcgcatagac gccgatatcg
     2941 aggagggcac caccggccag ggagcggttg aagaagcggt tgttcatatc gtactcttta
     3001 taactgccga aattcatctg aatgaaacgc agcggcccca gggcgccgga acggatgagt
     3061 tcagcgagct gtttgtaaat aggcatatga tataaggtca tggcttcggc caggacgacg
     3121 ccgtgttcat cagcacagcg ccgggcctgg tccagttcca ggctgttaag ggtaatggat
     3181 ttttcgcaga gcacgtgttt gcccgctgcc agcgccggca gcagataacg gatatgcgta
     3241 ttatgaggcg tcaaaatata gacgatatcg acgtcgggat ccgaaaagat atcctctggc
     3301 ttgtcgtaga ccttggcgat gccgtatttt tcagcaaaag acaaggcctt ttcatgggtc
     3361 cggttgccga cactgtacaa attcccgccc agggcctgca tagcctgggc cagttcattg
     3421 ccgatgacgc cgcagcccaa agtcgcccaa cgatatgctg gcataatgat tccccctttt
     3481 acgataacag gcggctgacg agcttgacac agtctgccgc atctttggaa tagccatcgg
     3541 caccgatttc atcggagaaa ctcggcgtaa cggctgcgcc gccgatgatg atcttgccgc
     3601 catactgttt atccgccgct tcggcgatga catctttcat gtgcatcatc gtcgtcgtca
     3661 tcaaggccga caagccgacg acagatgcct tttcgcgcag cgccgtctcg atgatggtgc
     3721 cggccggcac atccttgccc aggtcgatga ccttatagcc gtagttgcgc agcatgaggc
     3781 cgaccaggtt cttaccgata tcgtggacgt cgccttcgac ggtggcaatg acgatggtcg
     3841 ccatgtcctg ctggcccggg tctttcttga tgagcggttc caaataggca atggccgttt
     3901 ccatcgtatt ggcactggca atgagctggg gcaggaagta aatctgttta tcgtataaat
     3961 cgccgacctc agtgatggcc ggaatgagca ggtcgttgat gatggcatcc ggtgccatac
     4021 ccttggccag ttcttcctga gccaaggcga cgacggcgtc cttttcccct tccaggacag
     4081 cctggtagac cggattgacc ggcccttgct cagcgtcttg ggactccccc gccgtttcga
     4141 cagccccagc cgtaccgtgg ctggccagga gttctgcttc ttcctgccgt tttctttcgg
     4201 cttctttctt gtcgaaatac tgcatgcggt ggatgtaggc caaatcgctg ccctctttat
     4261 tcatgagcat attggccgct gctgccgtat tcatgagcaa atcctgggac ggattggcaa
     4321 tggccatggt caggccgctg gcaatggcca tgcccaagaa ggtggcgttg acatagatcc
     4381 ggtccggcag gccgaaggag atgttggaca ggccgcagac cgtagccagt cccaggtcct
     4441 gcttgcagtg agaaaacgtg gccatgcagt ccagggccga cgtcggcgca gccccgaccg
     4501 tcgctaccag ggcgtcgacg acgacatctt ccggcgaaaa gccgtggtcg taggcatcgg
     4561 ccaggagctc gtcgaggacg gcatgttttt cttcgagggt cttggggata ccgttatccg
     4621 aaacaggcag ggcgatgaac atggcgccgt actttttgac gacaggtagg agtttctttc
     4681 gcttttccgt ttcacaggaa atggaattga ccagggcccg gccgggatag atgcgcaggg
     4741 ccgcttccat gatgtccggt gagctcgtgt cgatgctgag gggcaggccc gatacgcctg
     4801 tcacttcgta aatggccttg agcatcattt ctttttcatc gatgccgttg gtccccatgt
     4861 tgacgtcgag gatagacgcg ccgttgcgtt cctgttcgcg ggccatggcg cggaccatgt
     4921 ccagcttgcc cgcccgcagt tcagcctgga gcttcttctt gcccgtcgga ttgatgcgtt
     4981 cgccaatgac ctggaaagag ccgtcgagct tgatttctac gacctgccgt tccgaggcca
     5041 ggacgcggtg atggctggca tgcggcgctt tcggcgtcag ccggcagaca gcctcgtgaa
     5101 gggcccggat atggtccggc gtcgtgccgc agcagccgcc gacgatatgg gcaccggctt
     5161 cgatgagcag ccggccgtcc tgggcgaatt cttccggcgt cgtcttatag accgtcttgt
     5221 caccatccaa ttccggcaaa ccggcattgg gcttggcaat gaggggaatc gtcgcatatt
     5281 catacatctt ccggaccgtt tccaccatgt cggccggtcc tgtcgagcag ttcaggccga
     5341 cggcatcgac accgaggttc tgcaagacga cgacagccgc ttccggcggc gtcccataga
     5401 gggtacggcc gtctttttca aaggtcagcg tcgccatgat gggcaggtcg cagacctcct
     5461 tgatagccag gacacaagcc cgcgtttcct ggaggctcat catggtttcg acgatgaaca
     5521 ggtcgacacc ggcatcgtac aaggcctggg cctgttcgcg atagacgtcg acgagaccgt
     5581 caaaggtcag ttcacccata ggatagagct gctggcccgt catggtcatg tcgccggcaa
     5641 ccagggcctt cccctgggcc gcttctttgg agatagcaac caagccctga ttgatggcga
     5701 cgagctggtc atcgaggccg tattgggcca atttgagccg gttccccgta aaggtcggcg
     5761 catagaggat ctgcgagccc gcttcgacga aacggcgctg caaatcgatc atgacgtcgc
     5821 ggttctctaa gatccattgt tccgggcaga cgccgatagg catgcccgcc tgttgcaggt
     5881 tcgtccccgt cgccccgtcg agcaggacaa caccgccctg tagaagttgt tgaaatgctt
     5941 cgttagtcaa tctaagtccc cctaaaaatt cagataatca atgtataagc gttgaaaggc
     6001 ttcgtcgttg ccgaggacga tttcctctgc cccggcggcg atggcctgta actcttccaa
     6061 agtcacggcg cccgtcaaaa ggccgatggc ccctttcagg gacgtgttgc cgacagccgt
     6121 cgtccgttcg gccagatctg ctggcaacag gccgatgaca gccgctttct gcggatccag
     6181 gtaataaccg aaaccgccgg ccaggaagac ctggtggacg cgctgacggc tgattccgct
     6241 gcgttcgatg agcgattcga tgccggcccg gatggtgctc ttggccatct ggatttcgcg
     6301 gatatcctgc tggcttaggg taatgcgctg ataatccagc gtcgacgcca aaggaaagcc
     6361 tttatggaaa taaggctcta cgagtttccc cgtttcgtcg accaggcctt cccggaccag
     6421 gccggccatg gcttcgagga tgcccgtccc gcagataccg acgggaacgg cgtggtcgat
     6481 agtccgcacc ttggcccggc cccgctcaat gcggacgccg caaatggccc cggggacgct
     6541 gcccgtcccc caggtcagtt tgcccccttc cagggccgga ccggccggag ccgatgcaat
     6601 gaagcgctgg tccttattgc ccaggaccat ttccccattc gtcccgagat cgacgaagag
     6661 gctgacgtcg tctgacgagg ccaggccgca ctgccagatg ccggccgtaa tgtcggcacc
     6721 gacgtaggtg ctcatgccgg gcagcagggg cactgtagcg tctgccagtg gcgactgcgg
     6781 tcccaggacc tcctgagcgc gataagtctt cccgcccagg gatacgggat ggaacggcca
     6841 gtcgcccagg ccgtcgcagg gccagcccat gagcaggtgg agcatggtcg tattggcggc
     6901 aatggccatc tggcggcagc gagcggccgt gccgggatgg tccttcagga gctgcttcat
     6961 cagccgcgtc aggtcccggc ggacggcctt ctgcaaggcc ttgcctttgc catggcaggc
     7021 ggcaccgatg cggctgacga catcggcccc aaaagagcgc tggctgttga ctgccgtcgc
     7081 tgtcgccagg atttttccat ccgtacagtc taccagagct gcggccaacg tcgtcgtccc
     7141 gatatcgaca gccaggccat agtcatggtc cgctgccagt gaaagggctc cctcgtcagc
     7201 ggcttcggca gccagagccg aaaagccctg ctgttcctgc acgggaacga caatttcgac
     7261 cgcttcacgg gttacggcct tgcaggccag gcgccagccc tgggccagct gagcttcgga
     7321 aaagatgcgc ttgtcttccg gggtgacggg aagccgcccc cggacgacgc ggacacggca
     7381 tttgccgcac atgcccttgc cgccgcaggg catagccagg gccgttccct gagactggat
     7441 atagtcatgg acccggcagc cgccagggca ggtcagccgt acttcccggg ccgattcggc
     7501 ctggcgctgc gggcaatccg tcttagggca ggccgtacag tcatgtttca cctggaatac
     7561 agccgggtct tcgcaaaggt caaagacgag gctcatggat ttctccggcg acaaagccat
     7621 ggccgcattg accgtcacgc cagccgtccg gccggcagcc gtctcacaga ccgcatcggc
     7681 ctggaccgat aagggcaaat cgctgcccgg ttcgtggcgg caaacgatgc cacagccctt
     7741 ttgccggcaa ataaggcgca gctgctgcaa aacctgctgt tccagggcaa agaggcacgt
     7801 atcagccatg gcagcgaaga gcacggcggc cagctcatcc ccgtcatcct gatagcggtc
     7861 gatctgccgg ctcacagcag cacctaacgt caggatgaca tagagccgtt ccgctgtaaa
     7921 ggccaggacc gccttggcct gcaaagaccg ccgcaagagc ggtgccatat cccggcacat
     7981 cccttcagcc cgttcccaag ctgccccttt cgattcataa ccagcaaacc gcagagcctg
     8041 ctccacgtcg gggacgatcc gctgcccccg gatgatacga atatctgcca tgtctatcct
     8101 cctaaaaata ttatctataa tacataatta tataacctca aggcgcctcg tggcaacaca
     8161 atcttaacag aaacctcacg gc
//
LOCUS       DAWABZ010000079         8082 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2407_length_8082_cov_3.258627, whole genome shotgun sequence.
ACCESSION   DAWABZ010000079 DAWABZ010000000
VERSION     DAWABZ010000079.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 8082)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8082)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8082
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2407_length_8082_cov_3.258627"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5992..6056
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1742 a         2356 c         2184 g         1735 t
ORIGIN      
        1 gtcgtggccg ggctggaaca tctcatcgat tatgtacaca atatacattt cagtgacgac
       61 gacctggcct atctgaagag caccggtttt ttccaggaca aattcctcga ttacctgaaa
      121 gacctgcgct ttacgggcga catctatgcc atgcccgaag ggaccgtcgc ctttcctcag
      181 gaagtgctcc tgcgcgtcca gaccaagaag gacgaagcct tactgttaga aacgtgcatg
      241 tccatgatca tgaaccatga aagcctcatt gccaccaagg ctcgccgcgt ccggacggtg
      301 gctggtaagg acaacctcat ggaattcggc ctgcgccggg cccaggggaa atcggccggt
      361 gtttacggcg cccgttcggc catgatcggc ggctttaacg ggaccagcaa cgtcctggct
      421 ggcgccaaat tcggcatgcc cgtcttaggg accatggctc acagctgggt catgagcttc
      481 tcgtcggaac tggaagcttt ccgcgcctat gcccgccagt acccgaacaa cctcatcctc
      541 ctggccgata catataatac gctgaaacag ggcgtccccg atgccattac gatcttcaag
      601 gaaatgaagg ctgccggcac cctgcccaag gcttatggta tccgcctgga tagcggcgac
      661 ctggcttatt tatcgaaaga agcccataag atgtttgccg atgcaggctt cccggatgcc
      721 atcattgcgg cctcgaatga cctcgatgaa aacctcattt ccgaattgaa agcccagggc
      781 tgcgccatca actcctgggg tgtcggcacg aacttgatta cggccaagga cagtccgtcc
      841 ctgggcggcg tcttcaaact cgtcggccag tatgacgatg gcaaattcgt gccgaagatc
      901 aagatgagcg acaatgccga aaagatttcc aatccgggcc tgaagaacgt cctgcgcatt
      961 tacgataaag atacggggaa aatgaaagct gacatcatca tcctcgaagg tgaaagcatt
     1021 gacgaatcga aagacctgct cctccagagc gataaggcac cgtggcgctc gcggacgatt
     1081 cctggcggga cttaccgtgt ccagaagatg ctcttcccta ttttccagaa aggcgaactg
     1141 gtctatcaga aaccggacct caaaaccatc atggcctatg cagaccagca ggtccagact
     1201 ttgtggcccg aatacctgcg cctggtcaac ccggaagaaa tgtgggtcca gcgcagcagc
     1261 aagctgtcgg ctttgcgcga taaagtcttg aaagaagaat cggctcattt cttttaggga
     1321 gatgattcca tgaagggagc tatcatcggc gatattgccg gctccatcta tgaattcgat
     1381 gaaaccgcgg aatccctgga ttttccttta ttcgttaaaa acagccgttt taccgatgat
     1441 acggtgacga ctgtagccgt agcggcagcc ctgatggagg ggaaggcatc ctgctgcggc
     1501 tatatcgaac ccttgcggcg ccagctccgc tactggtgcc ggaaatatcc cgacgccggt
     1561 tttggcggcc tgttccgccg ctggttcctg gccgacgagg caccggctta cggcagctat
     1621 ggcaatgggg cggctatgcg cgtcagcccg gctggctgga ttaccgatac tctggaggaa
     1681 gcccaggcgc tggcagagct gacagccgtc gtcagccatg accatcccca agccatcaag
     1741 ggtgccattg ccgtggcgtc ggctatcttc ctggcccggc aggggaagga gaaagaggcg
     1801 attgccgcat atctgcggtc gcatttctat gatctgtccc ggacactggc ccagatacgg
     1861 ccgtcttatg gctttacctg cacgacggac gatagtgtgc cagaagccat cgaatgtttc
     1921 ctggaaggga cggactatga agatacgatc cgcaaggcga tttggctcaa cggcgatacg
     1981 gatacgcagg cagccattgc cggcagcatt gccgaagcct attatggcgt gcccgatgcc
     2041 ttgtggcagc aggccctgcc atatgtcggt gacgaagaaa tgaagggcgt cattcgacag
     2101 ttctatgaaa actatattgg ttcgaggggg aactcttttg atttcaacag agacaagcaa
     2161 gaaatatgaa aaactccagc agatcttaaa ggacatgggc agtgtcgtcg tcggcttttc
     2221 cggcggcgtc gacagcacct tcctgaccta tgcggctcac caggctctgg gggaccgggt
     2281 cctggccgtg acggccgttt cggcgaccct gccgggcagt gaagcggctg atgccaaggc
     2341 catggccaag gtcatcggcg tcgaacaccg cctggcccat tcgacggaat tcgaggaccc
     2401 ggacttcgtc cagaatccgc cagatcgctg ctatatctgc aagaaaatac gctttacgtc
     2461 gctcgtggcc ctggccaagg ccgaaggcta tcagtgggtc gtcgacgggg ggaatgtcga
     2521 tgacctcggt gattaccggc cgggtatgaa agccctgcag gaattatccg acgccgtccg
     2581 cagcccgatg atcgaagcag gcctggccaa ggcggatatc cgggctttgt cgaaggaatt
     2641 cggcctccag acctggaata agcagagcgc tgcctgcctg gccagccgcg tcccgtatgg
     2701 cgtggaactg acgccgcacc gcctggctca gatcgatgcg gccgaagccg ccgtagcgcc
     2761 ttacgtccag gggtccctgc gcgtccgcta tcacggcgat gtagcccgca tcgaagtcgc
     2821 cgtcgatgaa atcccgtcgg tcctggctca tcgggaggaa atcgtccggg ccatcaagga
     2881 tacgggcttt acgtacgtca ccctcgattt gggcggctat gaaatgggca gcctgaacga
     2941 cgtaatccat actgatgagg aagtaaagtg atatggctat ttccaagaaa gtaaaagatg
     3001 aaatgctgca gcggttccag gaccagtacg gtatcatgaa accggctctg cactataatt
     3061 ctccttttga attgatgatt gccgtcatct tgtcggccca atgtacggat gaacgggtca
     3121 atatcgtgac ggccggcatg ttcccggaat ataacacgcc ggaaaagatg ctgaccctgg
     3181 ggacagcggg actggaagaa aagatacgga cctgcggcct ctatcgcagc aaggccaaga
     3241 atatcctggc caactgtgcc atcctttgtg aaaaatatca tggccaagtg ccgcagactt
     3301 tcgatgagct ggtgaaactg cccggcgtcg gccgcaagac ggccaatgtc ctggtcagta
     3361 tcttgttcaa tacgccggcc attgctgtcg atacccacgt cttccgtgtg tccaaccgtc
     3421 tgcagctggc ccaagggacg acgccgctgg aagtcgaaaa gggcctgcag aaggccattc
     3481 ccaaggaatg gtggagccgg gcccatcatt ggctcatctg gcatggccgc cgggtctgca
     3541 aagcccgcaa gcctttatgc gacaactgct ttttggctga cctttgtccc agttgtcaga
     3601 agtaaaacgt taaatcaaaa taaatacatt gacaagtcgt taaaaatttt ataaaataac
     3661 accaatacag acaaatttca taagttatat cgagagcagc tgagggacgg gcccgatgac
     3721 gctgcggcaa cctgcctaag gtaaggtgcc aattcctaca ggctattcct gaaagatatc
     3781 attatgactg aggcgcagct tggaggcagc gcctcttttc ttttggcgta ttatgtactt
     3841 attttcacat cattcaatca ttcttagggg gtattttcat gaacaacaac tcttctggct
     3901 gcaaggctat ccgggggctg gatgtgctca tcctgggatt cgccttcttt gccacctatt
     3961 ttggcgccgg caatttgatt ttcccgcccc agctcggttt gaacagcggc agcgatttcc
     4021 tgtccggcct ggctggcctg accctttcgg gtatttttct tcctatcttc acgctcatcg
     4081 tcatcggcct caacggcgac gtccactcca ttactgaacg ggtagggaag aatacgtata
     4141 acgtcctcct ggcagccctc atgctggtct gtaccttcgt ttccattccc cgtacgtgtg
     4201 cgacggccat ccagctcggc atccagggca acctgccggc agcaccgttc atcccgctgg
     4261 tcatcgccta tttcgtcatc agcttcttct ttgttgccga ccagaacaac gtcatggacc
     4321 gcatgggcaa atacctgacg ccgctcctgg ccctgatcct ggtcatcgtc gccttcgtcg
     4381 gcatcttcaa tcccctgggg acacccgtcg ctccggctgt ggaccatccc ttcgtcaatg
     4441 ccttcctcgg cggctataac acgggcgacg tcctggtcag cttcatcatg gcagctgtct
     4501 tcatcagctc catttacggc aagggctata cgaccgtcgg ccagcgcaat aaagtgctca
     4561 tctactgtgg catcgtttcc ttcgtcctgc tgctcatcat ttacggcagc ctgctctaca
     4621 tgggcgcctg cgtcagcggt gactatgcgc agaacatcgg ccgtgcggaa ctcctggtcg
     4681 ccatcatcca gcgcgtcggc acgtgggtca tggttcccat gggcatcgcc gtcgtcctgg
     4741 cctgcctgac gacagccatc ggtcagattg ccgccgttgc cgaattcacc agcacggcta
     4801 cgggcaaccg catcagctat aagcaagtcg ccgtcgcctg ctgcatcttg tcggccctga
     4861 cggccctcct cggcgtcgac ggtatcgtca cctacatcgg ctggattttc ggcgtctgct
     4921 atccgccgtg cctggccctc ctggtcctgg gcatcatcgg ccgcttcatg cccaacaacg
     4981 gtgcttacaa aggctctgtc tacctggtca cgctcttcgc ccttttggaa tccctgccgg
     5041 gtctgagcag cctgggcttt gctaaagcca tcgtcgccgc tacgcccctg tccacatacg
     5101 gtttcggctg gatcgtcccc tttatcatcg gcctcatcgg cggctccatc atcggccatt
     5161 tcgtatccgc ctcagaaccc gtagaagccg aagcgaaata agatagtttg tggtatggag
     5221 tctgtagggg gcgccactaa aaatagactg ccttttaggc agtctatttt tttccccgac
     5281 ttgcataatc aatgacttat ctctataatt aaagaataat ctcgagtaaa agggggtaat
     5341 ttcatggatt tgaaaaaact atatatcgat ggccagtggg ttgcgccctt gtctggccaa
     5401 tatattgacg tagaaaatcc ggcgacgaaa gaaatcttcg ccactgtcgc cgcggctggt
     5461 gccgatgatg tcgctgccgc tgcccaggcg gctgccaaag ctttcccggc ctggtcggct
     5521 atgccgctgg aagaccgcat caccttcatg aagaacctgc tggtccagat gatcagattg
     5581 gaaccgcagt tcgtcgacgc cgtcgtcaag gaattagggg cgcctgtagc ctttgccagg
     5641 accaaccata ttggctacca gttcgtgcgg acccagtcgt atatcgactt ggcaccggaa
     5701 ttgccactgg tcgaacatct gtcccaatcc gtcgtctacc gccggccttt gggtgtcatc
     5761 ggctgtatca cgccgtggaa ttatccttta gggcagatta tccagaaagt cgtaccggcc
     5821 ttgctcgtcg gggcgacagt cgtcctcaag ccgagtcagc acacgccggt gacggcgtac
     5881 tacctggccg aagcctttga ccaggccggt ttccccaaag gcgtcttcaa tctcgtcgcc
     5941 ggccggggca gccaggtcgg caatgccatc tgcgacgacc cgaatatcgc cnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaggt
     6061 atctcagcgc gccttagctt cgatgaagcg catcagtatg gaactgggcg gcaagtcgcc
     6121 tttcgtcctc ttgaaggggg cggactacga aaaggctatc cgcatgtgct tcaattccat
     6181 tttcctcaac agtggccaga cctgtacggc cctgtcgcgc ctgatcatcc cgcgggatga
     6241 caaggaacag attgaacagc tcatgctgaa gatactgccg gaatataccg taggcgaccc
     6301 ggctctggaa acgacgaaaa tcggtcctgt cgcatcgtat gaccagtggc agaccgtcga
     6361 tgcctacatc gccaagggcc tggaagaagg ggcgacactt ctggctggcg gccgtccgga
     6421 tgcgccggac cacggttact atgtcaagcc tgtcatcttt acggacgtaa ccaatgacat
     6481 gaccattgcc cgcgaagaaa tcttcggccc cgtcctcagc gtcatcgctt atgataccgc
     6541 cgacgaagcc ctggccattg ccaacgatac gccttatggc ctcaacgctg ccgtctgggg
     6601 accggacaag gaagaagcct cgaaggtggc agcagccgtt ttgtcgggca acgtctacgt
     6661 caacgacagc ccgcgcgacg tcacagcgcc tttcggcggc tttaaggaca gtggtatcgg
     6721 ccgtgaaggc ggcaaagatg ggatgttgga atttacagaa ccccaggcca ttttttatgc
     6781 ataaacagtt ataggaggag ttttagcgtc atgaatttcg atgaagaagc catgaaaatc
     6841 cgccgcggca aacacggcgt tattgaaacc attccccggg aagtcctcaa gacccacgac
     6901 gacctgagta ttctctatac gcctggcgtt gcccagccgt gccgggaaat cactaaggac
     6961 ccggatttgt ctttcgactt gacctgccgt ggcaatatga tcgccgtcgt atcggacggg
     7021 acccgcgtcc tcggcctggg cgacatcggc ccggaagctg ccatgccggt catggaaggg
     7081 aaatccattc tcttcaaggt ctttggcggc gtcaatgccg tacctatctg tctggatacg
     7141 aaggacccga aagaattcat cgaaacggta aagcgtttgc agccgtcttt cgccggtatc
     7201 aacctggaag atattgcttc gccgaaatgc tatgaaatcg aacagacctt gaaacgggaa
     7261 atggacatcc ctgtcttcca tgatgaccag cacggcacgg ccattgccgc cctggccggc
     7321 gtcatcggtg ccttccgcta tgtccataag gacttgaaga cagcccgcat cgtcatcaat
     7381 ggggcagggg ctgccggcgc ttccatcggc cgcctcctgg tccgcgccgg agctcggaac
     7441 gtcaccatgg tcgaccgtcc ggggattctc tacgctggca tggaagggct gaacccggtc
     7501 caggcggaac tggcatccct gaccaatccg tcccatgccc acgggaccct ggctgatgcc
     7561 gtcaaagggg ccgacctcct cctaggcgta tcggcaccgc atatttttac taaggaaatc
     7621 atccaggcca tggccgacga tgccgtcgtc ttcgccatgg ccaaccccat cccggaaacg
     7681 gactacgcct ctgccaaagc ggccggtgcc aaagtcgtcg gcactggccg gtcggatgca
     7741 ccgaatcaga tcaacaacgt catggtcttc ccgggtatct tccgcggcgc cctggccgtc
     7801 cgggcccgca gcatcaacga agacatgaaa ctggctgcgt cttacgccat cgcctccctg
     7861 gtcggcgatg acgaactggc cgaagaccac gtcatccccg acgccttcga ccaacgcgtc
     7921 gctccggccg tcgccgcagc cgtcgccaaa gctgccatgg aaagcggcgt cgcccgcatc
     7981 acccgcgacc cggaagaagt ccgccaggaa gcggcagaca ccatcgacaa actccacgcc
     8041 atgctcgact tgatccacgg gtaggggcta gcagttagta at
//
LOCUS       DAWABZ010000080         8046 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2419_length_8046_cov_2.841447, whole genome shotgun sequence.
ACCESSION   DAWABZ010000080 DAWABZ010000000
VERSION     DAWABZ010000080.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 8046)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8046)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8046
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2419_length_8046_cov_2.841447"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2078 a         2135 c         2328 g         1505 t
ORIGIN      
        1 gttagatagc agcgtaggcg gggccatgac gaaagccatc gcccagacaa agcagatgca
       61 ggcgcagatc cgcgcggcga atcgcgaaat ggccagcctg caaaagcagg cagccaagca
      121 gcaggccagt aagggctatg tcgagtacga caccgaactg gctatgctcc aagcgtccgc
      181 taaaaagaat caggccgcga aagagtacga ggccacgatg gaccgtatca acgcaaagca
      241 gaaagcgtcg gcgaatctgt cggccgctgt cagtaacctg aaagccgggg ctgtcgcggc
      301 ggctgccgtt gccgcgccgt tggggcttgc ggtcaatgaa gccattaagt ttgaatcggc
      361 tatgtctgat gtgcgtaaag tcgtagactt tgacacgccg cagcaattca aggaaatggg
      421 cgacgacatc ctgcggatgt cccaggaaat gccgatgtcg gccgaaggta tcgcgaaaat
      481 tgttgccgct ggcgggcaag caggtatcgc gcgggatgat ttgaagcaat tcgcgaccga
      541 tgccattaaa atgggtgtag ccttcgacat atcggcagag caggccggcg atatgatggc
      601 caaatggcgt acagcctttg gtatggacca ggggcaggtc gtccagctgg ccgatcaggt
      661 aaactacctg tcgaatacga cagccgccag cagtgatgcg atttctgaca ttgttacccg
      721 cgtagggcct ttgggcgacg tcgccggtat cagcgcctcg cagattgcgg ctattggtgc
      781 atccatggcc gccgtcggcg tcaagtccga cgtcgcggca acgggtatta aaaacttagc
      841 tctaggcttg gtagccgggg cgggtgctac gaaatcccag caggcggcct ttgcacaatt
      901 aggcctgtcg gcagaagacg tcgcaaagcg gatgcagact gacgcccaag gtactatcgt
      961 agatatctta tcgcgcatta aacaactgcc aaaagaagca caagcggcga cgttgagcga
     1021 cttattcggt aaagaatcta tcgaagccat cgcgccgctg ttgacccagc tggataatct
     1081 aaaggacaat ttcaacaaag taggagacgc ctcgcagtac gcgggctcta cggaagccga
     1141 ataccaggcc agggcgggca cgacagcaaa ccagctgcaa ctggctaaaa ataatttagt
     1201 cgctttagct gtcaatctcg gcagcctctt gcttccggcg gttaaggccg tagcaggcgg
     1261 cgtggcccgg gctttaggcg ctgtcgctca gtttgtgact gagcatcagc agttggcgtc
     1321 ggtgattaca ggtactatcc tggctattgt agggttgacg atggcggccc tgggcatccg
     1381 tgctgccgtc gcctactaca agtacatggt agcgacaatt aacatggtcc gcaacgcaca
     1441 cattgcagca tctattgcta caaagatttc tacggcgtct acgattgcag ccgcggcggc
     1501 tcagcgagcc tttgctatcg gcgcgcgtat tgccgccgtg gcccagatgg ccctgaacgc
     1561 cgtcatggcg atgaatccat tcacgctcct agtcatcgct atcatgattg tcgtggccgc
     1621 gctggtatat ctgtggaata caaacgaaag cttcagggca gcgtgcatcg cggcgtggga
     1681 atccatttgc gctgccgtat cgtcggcgtg ggatacaatt tcctcggcgg cggccgcggc
     1741 ctgggattac atcaccagtg ccgtatccgg ggcatatgcc tttattgtcg gctgctttga
     1801 ttctatttcc gctgcggcgc aggccgtgtg ggatgcagca gtcagcgcag ctcagagtgc
     1861 ttgggatagt attacatccg ctgttgacgc tggcgttcag tggtgcatcg acaaatggga
     1921 ggccctaaaa gaagcttttt cccatcctat cgacgccatt gtgaatttca tcaagggcgg
     1981 tgacggcgac gcagcctctg cggccggcat gtctgccagc ggcggtgtct tcaaccaccc
     2041 gtacctgacc tgggtagctg aagcggggta cccggaagtt attgtcccga ttacgcacga
     2101 cgccaatgct tataatctct gggcaaccgc gggccagatg ctcggcgtcg gccaggccgc
     2161 gatgccgacc atgccgaata tgcctgctgt ggcgccgtct tccggcgggc cggtagaaat
     2221 caacttcgcc ccgaccatca atgtacaggg cggcccaggg agcgaacagg ccgtttcacg
     2281 tctgatggac cagaagatga gagaattcga cagcatgatg aagcggtggg ccgcaaacca
     2341 gaggaggctg agctttgagt agcacataca acaccatcca gggagatatg tgggatctca
     2401 tttccttccg ggtctacggc tcggagaaat acgtcggcga gctgctggac gccaacccac
     2461 agtacagaga cgtcacggcc ttttcagcgg gaatcacact gacctgcccg gacatcaaag
     2521 ccggcacgca taaagtcatg ccgccgtgga agaggtgatt ggatggcttt actgcaaaag
     2581 atgagggccg agatggaacg gctgaagaag caggccatgg ccggcgtcgc cgcacaggaa
     2641 cacatggccc gccgcgcctg gctccaagta atttacagca acaaaggcgc cgacggcaag
     2701 gacatttcag agaccttggc tgaatacctg cttagcgtaa gctatacgga caatttatcc
     2761 gggcaggtag acgacatttc cctgacgctg gaagacaaag ccgggctctg gcagggcgac
     2821 tggatgccga ctaagggcgc cacattggac gtcacgctgc acacttacaa ctggtacagc
     2881 ctttacgaag gcgaaatcac tgtcagtctg ggcatgttcg acgtcgacga aatcgagctg
     2941 tcgagcgccc cggatgtcgt taccattaag gccgtggcca tcacagtagg agacgacagc
     3001 acactgagaa gtaccttgca ctcacggact tgggaaaaca tcagcgtgca aaagctcgct
     3061 aatgacattg cccaggaaaa cgagatggat ctgagctggt actgcgatga cgatcccatc
     3121 attgacaagg tagagcagga cgaagagtcc gatttggaca tgctgcagaa agtctgcaat
     3181 gatgccggct ttgccctcaa agtaacgacg aacgaaatca tcatctttga tgaggcagcc
     3241 tacgaagaag aagacccgct catagaaatc taccatccgg acacgcagaa agtcatcgac
     3301 atcagtgatg aagacgacga ggcaaagccg gaacggattc tgcagaacac gggctattct
     3361 ttcaagtcga agctccgcga cgtctactgg gcatgccacg tcaaatatgc caaagataaa
     3421 gacaaggccg tcattgaagg aacgttcgtg gacccgaata aagaaagcgg cgccgtactg
     3481 gaggtcaacc aggaagtcaa ttctcaggcg gaagccgaac ggctggcgaa gaagaagctg
     3541 cgcgaaaaga accgcgagga gtacaccggg agcttcaaca ctataggcca accattcctc
     3601 tgcgccggcg aaaccgtcga gatgataggt ttcggaactt tttccggaaa gtacatcatc
     3661 acccaggcaa agcatgacct cagcagcagc ggcttcgtca ccagcattga aataaggagg
     3721 tgcctcattg gctactgata tttataaact gctgtcccgc tgcgtccgcg tagggagagt
     3781 atcgtcggta gacacagcgg acgcctcggt cagggtaacg tttccggaca gggacaacat
     3841 cgtttcaccg ccactgaaag ttgttatgcg cggctgcaag gcggacaagg actattggat
     3901 gcctgctgtc gacgaccagg ttctctgtct gttcatcgcc aacggtggcg gcaaaggaag
     3961 cggcacgggg tacatcctgg ggactatcta cagctctgta gacccaccgc cgggcggcgc
     4021 ctcgcgagta ctgaacattc cgggcagcat gaccatcaac tgcggcagcc tggtcgtcaa
     4081 tgccagcggc ggagacatca tagtaaacgg tatttccctg gtcaaccata cccacggcgg
     4141 cgtagaatcc ggcggcagta agaccgggaa gccgacgtaa ggaggcgaga gtatgtatat
     4201 tggctatttc ggcacagtca tcttcggcgt cgccgaacac tacctggtga cgccggatga
     4261 ggtggaaagg agcggccagg cgcgctggca gacacatgac actatcatga agaagcccgt
     4321 gccacagttc atcgggccga gccaggaaag tatttccttt aagatgcacc tcatgacgct
     4381 gtacaacgct atgccggaac aacagctgaa gaagttgcgc gaaatgcgcg ataatggcat
     4441 tgtttgcccc ttgatcatcg gcggcgtccc ggtctcacag aactactggt acctggagaa
     4501 tttgagcgag agcgattcca tctacaatgc atacgggaaa attatcgctg tcactgccaa
     4561 cgtacaactc aaagagtacg acacgaccaa cacggacgaa gaatccatgg tgaacaaagt
     4621 aggcaaggca tataacgtcc tcactacctt gttcgggaga ggaggcctgt aagtgcggta
     4681 tgaagtaacg gcaaaagacc cggacacgat tgattttgca ccgaagaacg aagtggaaga
     4741 agtcctacag aatgttcgca ccatattgtc gactgctaag gggactgtgc cgctggacag
     4801 gcagttcggc ttggacgggg ccatcatcga cctgccggtg acgttggcca aggcgaaact
     4861 gacaaatgaa atcttccagg cgctcaaacg atacgaaccg cgggtgaaag tggaaaaaat
     4921 aagctttagc ggcgacatct cgggacagct gacgccgaaa gtagaggtga gcatcaatgg
     4981 atctgagtaa gctgaaagac gtctcatttg ctgactacgg cgaagagacc gtaaaaaacg
     5041 cgctgattag cgcctatgaa agcgtgagcg gccggacgct ggcgacaggg gaccctgtac
     5101 ggcttttcct gctgagcatc gcggccatca tcattcagca gcgtttcctg attgaccagg
     5161 cggggaagat gaacctcgta gcgtatgcca aaggggacta tctggaccaa ctgggcgctt
     5221 acctggacgt tgagcgaatt cctgcgacag ccgcggaaac aactctgcag tatacgctgt
     5281 ctgcggtgcg gccggaagca gtcgtcatcc cgaaaggaac caaagtgacg ggcgcgaaga
     5341 aagacgtttt ttttgcaacc gagaatccac tggtcattgc ccccgggtcg ctgagcggca
     5401 cggtagacgc acagtgcacc agcgtagggc tcacgggcga gggcctgccg gcaggatacc
     5461 tgacagaaca ggtggacccg ctgccgtacg tcgccagcgt caccaacacc acgacgacgt
     5521 ccggtggcgc agacatagaa acggatgact cgtaccggga acgaatccgc gaagccccgg
     5581 aaaaattctc cgatgccggg agttacggcg cctaccaatt ctgggccaag tccgcgtcgg
     5641 ccgacatcat gacagtgggt gtatccagcc cgacggcggg gacggtgcag ctgattccgc
     5701 tcctcaaggg cggccagatc cccggcgaga acctgcggaa acgagtcctc gaaatatgca
     5761 gcgccgaaaa agtacggccg ctcacggata atgtaacgtg tatcgcgccg acggaagtga
     5821 gctacgacat cgacgcaacg tataaaatca acacggatga cgctgcagca atcactgaca
     5881 ttcaggcggc cgtcaccaag gccgtagatg actatatcac ctggcagcgg tccgatttgg
     5941 ggcgcgacat cgacccgtcg aagctgtacc agctcatggt agatgccggc gccgtaaagg
     6001 tgcagattgc taaacccacg ctcacagcac tgacagacga ccagctggcg gtcagcggaa
     6061 aaaagacggt aacgtttgga ggcctggcaa atggataaaa aactggaaga aatcatcccg
     6121 caatctatcg cccaggacaa gaccgtccaa gatctatgca aggtcttaga tgaagaaatc
     6181 ctacccatcg ccacgggccc gaaaaaagcc ttggtgtaca gccggattga ttcgctgcct
     6241 atcgaagccg ttgacctgtt ggcctggcag ttccacgtag atgtatacga tatagccacc
     6301 gacgacacgg caaagcgtca ggccgtcaaa gacgccatta gaagccatcg atataaaggc
     6361 actccatggg ccttgaaaca ggcaatcaga cgggtcctgg cggatggaga cattacggag
     6421 tggcaggaat acagcggcaa gccgtattac ttcaaagtaa cgacagccga gggcctgacc
     6481 agcgcaaaac acttgcaaga cctgctgcgc gtcgtcgcag acagcaagaa cgtgcgctcc
     6541 tggctggacg gcatcgcaca gaccgttaat ggtcggggaa tcatttacac cagcggccgc
     6601 cggcggctgc agcggaagat gacgatttac ccgtccgaac gaactgaatg gaccaattcg
     6661 tcaactatct atgttgggat tgtccgacat gaaattgaag aaacagagat aaggggggat
     6721 ttccgtggcg atttggagta aattcgcgct gacaaacaac ggcaaaacgc tgctgacaaa
     6781 acatgtcgcg gaccagaaga ccttcactat caccagagtg gccactacaa atacgacgga
     6841 tgcgctggac gtcaaaagcc tcacgaaact accaggcaat gaacgttttt tcgggattat
     6901 gtcaaacacg cttacgaacg acgaagaaat caaagtggaa tgtatgatta caaacgtcgg
     6961 tctggctgaa ccgtacaaac ttacggccat cgggctctat gcaaagctca gcaatgatga
     7021 aacagacaca ctttttgctg ttacgaccgc tacggaccca gacactatcc cggcactcag
     7081 caatggtgtc gtaaagctgt acacagaaaa gattttcctt gccttcgaca gcaccgaaga
     7141 cgtgagcgta aagattgaca tggacactta cgtcacccac tcggaatgca tcgaaacagc
     7201 gtaccctgtc ggtactgtcg gacagacaga cggatcatac aagccggcgg acaaatgggg
     7261 cggtgtctgg cagcagatat ccggcgtata cctcatggcc agcggcgtct ataacggcac
     7321 aacctgccag gtcaataatt atgccggcga atatacccac cggcagacgt acgatgaaat
     7381 gttcccacac ggccatacca gaggcacgat gaatatcacc ggcggcttcc cagcggacga
     7441 ctcgcaggtt ggccgtcact cgggccagca ctacccatgg ggcgcatttc ttgtgggcgg
     7501 cgctgacggt attgacctgg actcggatac gggcgaaggt tcatacatca acttcgatgc
     7561 atcacgcaca tggactggcg tcagcagcac cgaaggcggc ggcaaaccgt ggccggtcat
     7621 gccaagggca tatgttgtcg atgtctggcg gcgggtgaag taaggaggga gagagatggc
     7681 gtttttgacg tcggggtata tcctgactac caaagggaga gaactgcagg cacgagtcga
     7741 aaaaggagaa acactgaacc tgacacgcgt caagctgggc agagggaaag tagaaacact
     7801 cactgactac gcggaaaaga cggacattgt cgacccggtc tgctcgctac tcatctcgaa
     7861 gaaagaagtg caagaaacgt cgtgcatctg cacggcatcc gtctccagcg agggcatcgc
     7921 cacaggcttc aacgctacgg agcttggaat cttcgccaaa gatggggacg atgaagtgtt
     7981 gtatgccgtg tcatatgaag gcaccagccc gacctacaca cctgggaaaa atgacaacgt
     8041 gagtgt
//
LOCUS       DAWABZ010000081         7953 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2463_length_7953_cov_3.687516, whole genome shotgun sequence.
ACCESSION   DAWABZ010000081 DAWABZ010000000
VERSION     DAWABZ010000081.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7953)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7953)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7953
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2463_length_7953_cov_3.687516"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1950 a         2399 c         1991 g         1613 t
ORIGIN      
        1 ccttacgtta cagttaccgt cattccctca ttatactaga agcgggctca ccacgtgcga
       61 gcccctacaa ttgcgcagac aggcaataac taattcacgg cctgtagcct tgcgcataaa
      121 tgtatgcaaa aaagggcttg ttgcacatgc gacaagccct ttttctatgc ccaaatgtaa
      181 cagttctctt tctgtcattg caaaaaaagt gtagatacct aacaaatttt tagctctttt
      241 ctattgcaaa tctgtaaggt catgttatac tctaggtata caaaaacttt tagatttact
      301 aaaatatttt tttcagttta tctttaggag gtacggatat gaagaaagat atgaaatggg
      361 ctatgaacca tctgcccaaa tcagatgata aaaatttgcc gatcatgtcc ctcagcgaag
      421 tcgccaaggc ccggacattc caccagagtt tcccgcaata cagcgaaacg ccgctgaccc
      481 ggctcacgca gatggcccgc tacctgggcg tcggttcgat tttcgtcaaa gatgaatcgt
      541 accgcttcgg cctcaatgcc ttcaaagtcc tgggcggttc ctattccatg gccaagtaca
      601 tcgccaagga aacgcaccgc gatgtatcgg acatgcctta tgacgtcctg acgtcagatg
      661 agctccgcca ggaattcggc caggcaacct tcttcacggc aaccgacggc aaccacggcc
      721 gcggcgtcgc ctgggccgcc cagaagctgg gccagaaagc cgtcgtccac atgccgaaag
      781 gcacgaccca gacgcggctg gaaaacatca agaaactcgg cgccgacgtc gacatcctcg
      841 acctcaatta cgacgattgc gtccgccagg cagccaaaga agccgccgct acgccgcacg
      901 gcgtcatggt ccaggacacg gcctgggacg gctatgaaga aatcccgtcg tggatcatgc
      961 agggctacgg cacgatggcc atggaagctg gcgaacagct gaaggcccgg ggcatcgaac
     1021 ggccgacgca catcttcgtc caggccggtg tcgggtccct ggccggtgcc gtcgtcggtt
     1081 acttcgccaa cctctataaa gacaatccgc cgaaattcat cgtcgtcgaa gcatcggccg
     1141 cagcctgtct gtataaaggt gccgcagccg gtgacggcaa gccccgcatg gtcggcggtg
     1201 acctggatac catcatggcc ggcctggcct gcggcgaacc gaacacgatt tcctgggaca
     1261 tcctcaagaa ccatgtgacg accttcatcg cagccgatga ccccatcacg gtccgcggta
     1321 tgcgtatgct ggcagcgccc ctcaaaggag atgcgcccat cgtatccggt gaatccggtg
     1381 ccgtcccctt cggcaccctg gctacaatca tgttatccga tgaatgtaag gacctgcgcg
     1441 ctgaactcgg cctcgacgac cattcacagg tcctggtctt ctcgacagaa ggcgacacgg
     1501 acccggaacg ctataagaat atcgtctggc gcggtttgaa taagtaaaac attatataag
     1561 atataggagt gattttcatg acagtaccgt acgaaaaaat caaagaagcc gccaaaggct
     1621 atgaacagga tatgacccgt ttcctgcagg acatcgttaa attccccggt gaaagctgcg
     1681 gcgaaaagga acatatcgac cgcattgccg aagaaatgcg caaactcgat ttcgataaag
     1741 tggaaatcga ccccatgggc aacgtcctgg gctacatggg caccggcaag accctcatcg
     1801 gcttcgacgc ccacatcgac accgtcggca tcggcaacaa ggccaactgg gatttcgatc
     1861 cctacgaagg ctttgaatcg gaaacggaaa tcggcggccg cggcacgtcg gatcagctcg
     1921 gcggcatcgt ttctgccgtc tacggcgccc gcatcatgaa ggacctgggc ctcctatccg
     1981 acaagtacac cgtcctggtc accggtacgg tccaggaaga agactgcgac ggcctctgct
     2041 ggcagtacat catcaacgaa gacaaggtcc gtccggaatt cgtcgtctcc acggaaccga
     2101 cagacggcgg catctaccgc ggccagcgcg gccgcatgga aatccgcatc gacgtccagg
     2161 gtgtatcctg ccacggcagc gcacccgaac gcggtgataa cgccatttac aagatggccg
     2221 atatcctcca ggacgtgcgg gccctcaacg aaaatgacgc agccgacacg acggaaatca
     2281 aaggcctggt caagatgctc gacaagaaat acaatcccga atgggaagaa gccaactttc
     2341 tcggccgcgg tacagtcacg tgctcccaga ttttctatac ctcgccgagc cgctgcgccg
     2401 tcgccgattc ctgtgccgtc tccctggacc gccgcatgac ggccggcgaa acgtggcaga
     2461 gctgcctcga tgaaatccgg gctctgccga gcgtccagaa gtacggcgac gatgtcaaag
     2521 tcagcatgta tgaatacgcc cgcccgagct acaccggcct gacctatccc atcgaatgtt
     2581 acttcccgac atgggtcatc ccgaaagacc acaaagtcac caaagccctg gaagaagcct
     2641 atacctccct cttcggcgac agccgcatcg gcactgccga agcggaagcc atgcgtaaag
     2701 cccgccccct gacggataaa tggaccttct cgactaacgg cgtttccatc atgggccgca
     2761 acggcatccc cgtcatcggc ttcggccccg gcgcagaagc ccaggcccat gcaccgaacg
     2821 aaaagacatg gaaacaggac ctcgtcacct gtgccgccgt ctatgccgca ttaccttctg
     2881 tatacaccga taaatgatca catcccataa cgattaaaag gagactgccc tatgaaaacc
     2941 ttacaggact acatcgataa attaaatgcc ctcaatttca aagacatgta tgaaaacgac
     3001 tttttcctca cctgggacaa gaccgatgac gaactggaag ccatctgggt cctagccgac
     3061 gccctccgct tcatgcggga acacaatatt tccacaaaga tttttgaaag cggcttaggc
     3121 atttccatct tccgcgataa ttcgacgcgg acccgttttt ccttcgcctc ggcctgcaat
     3181 ctcttaggcc tgcaggtcca ggacctggac gaaaagaaga gccagattgc ccacggcgaa
     3241 acggtccggg aaacggccaa catgatctcc ttcatggccg acgtcatcgg catccgcgac
     3301 gacatgtaca tcggcaaagg tcatgcctac cagaaagaag tcgtcgattc cgtcacgcag
     3361 ggctataaag acggcatcct cgaacagaaa ccgacgctgg tcaacctcca gtgcgacatc
     3421 gaccatccga ctcagtgtat ggccgacgcg gcccacatca tccacgaaat gggcggcctg
     3481 gaaaacctga agggcaaaaa aatcgccatg acctgggctt actccccgtc ctatggcaaa
     3541 cccctctcgg tcccgcaggg cgtcatcggc ctcatgaccc gtctgggcat ggacgtcgtc
     3601 ctggctcatc ccgaaggcta tgaagtcatg ccggacgtcg tcgacgtcgc caaaaagaac
     3661 gccgaaaaat cgggcggctc cttccgcatc acccacgaca tggccgacgc cttcaaagac
     3721 gccgacgtcg tctatcccaa gagctgggct cccttcgccg ccatggaaaa gcggactaac
     3781 ctctacgccg ctggcgacca ggccggcatc gacgccctgg aacaggaact cttagcccag
     3841 aacgcgcagc ataaagactg gtgctgtaca gaagaattga tgaagaccac gaaagacggc
     3901 aaagctatgt atctccactg cctgccggcc gacatcaacg gcgtcagctg taaagacggc
     3961 gaagtcgaag cctctgtctt tgaccgctat cgcgattccc tctataaaga agccagctac
     4021 aaaccctatg tcatcgccgc catgatcatg ctggccaaat gccaggatcc ggctcagacc
     4081 ctgaaagcct tagaagaacg cggcattctg cgcaaaatga aataatttga ccgccaaagg
     4141 tgccggtcct ccggaccggt gcctttttca tctccctgcc atgcgtttta ctcatcattt
     4201 ataaaacttt ttcacacata aaaggagatt tatcatgacc gagaaaaaga aacgtatcgt
     4261 cattgccctc ggcggcaacg ccctgggaaa tacactgcac gaacagatgc tggccgtcaa
     4321 gacgacagcc aaagccatcg tcgacctcat ccaggaaggc tgtgaagtca tcgtcgccca
     4381 cggcaacggc ccgcaggtcg gcatgatcaa ccacgccatg gccgccctga gccgggaaga
     4441 cccgaaccag cccaacacgc cgctgtcggt ctgtgtcgcc atgagccagg cttacatcgg
     4501 ctatgacctg cagaatgccc tgcgcgaaga aatgtacaac cgcgacatct tcgacatccc
     4561 cgtcgccacc atggtcactc aggtccgcgt cgacccggaa gacccggcat tcaagaaccc
     4621 gtcgaagccc atcggccatt tcatgaccaa ggaacaggcg gaatacgctg ccaaagaata
     4681 cggctacatc gtcaaggaag atgccggccg gggctggcgc cgcgtcgtcg cctcgccgct
     4741 accccaggaa atcattgaaa tcggcgccat ccaaaccctc gtcgattccg gccagctggt
     4801 catcgcctgc ggtggcggcg gcatccccgt catgcgccac ggcaaccacc tcaaaggggc
     4861 cagtgccgtc atcgacaagg attttgccag cgaacgcctg gccgaagacc tccacgccga
     4921 ctacctcatc atcctgacag ccgtggaaaa agtcgccatc cattttggcc aggcagacga
     4981 acagtggctg tcggaaatga cgaccgacca agcccggcaa tatatggctc aaaatgaatt
     5041 cgctcccggc tcgatgctgc ccaaagtcca ggctgccgtc aaattcgccg aatcccgtcc
     5101 cggccggaca gccttgatca cactgctcca aaaagccaaa gaaggcatcc tcggaaagac
     5161 ggggacccgc atttaccggg gcgcaaggat ctgactgatt ccctgttgca cgtaatggat
     5221 gaatttcttg actgccggtg atgccccatc ccggctggga atggcaatgc caatggtaat
     5281 cgtatggggc ggatccatgg gaaggatagc cacgcggccc tgccactgtg ccgtcatgag
     5341 gcgattgttc atactgacac ccaggccggc ttcgaccatg cgataggtcg tatagttatc
     5401 gatggccgta tatcgcgttt cataagaaat atgttcttcc ttaaggatcc ggacgacatc
     5461 cgtatcctgc tgcggcagtg gttcaataaa agattcatga cacaactgct tcaacgggta
     5521 tacctcggct cgtgccaagg gatgattttc cggaagccac atgaccattt catcttcaca
     5581 taagggaatc cagtctccgg aaaaagggcg ccacgtcgtg atacaacaat cgaagcgccc
     5641 ttcttccaat ccatcgtaca tcactttatt ggcgcattcc tcgatgtgga cgtggactgc
     5701 cggataggcc tgctggaaag cctgtatgat ctgcggcagc cagttggcgg ctacactgaa
     5761 ataagagcca atgtaaatat ccccggccgt caatccccgg atctgggcac tgtactgcaa
     5821 caaagcatct tcatcacgga tgaggcggcg cagataaggg agcactgcct gcccttcacg
     5881 ggtcagataa atgccttgtg ccgtccgccc cagcagggga aagccgattt ctttttccag
     5941 cgatgcaatg gaccgcatga cgccagacgg ggtatatccc aatcgttcgg cagctgaaga
     6001 aaaactgccc gcctggatga cttgcagcaa gagcctgcat ttttccgtat tcatatctat
     6061 cacctttgct tttttatcaa aactatatat attattttgc tattctctcc atactgcttt
     6121 tatcgtatca taaaagcaag aaaaaagtaa ggagggactt tttcatgaaa caaagaaatg
     6181 cagaaatact gctcatcatc gtcatcgctt cgcgcagcat ttcctatctc ctgtcgaaaa
     6241 tcggactgaa cgggataccg cctttagaat tactggcaat ccgcttcgcc ctgacgacac
     6301 ttatcctgct gctgctcttt caccgccgcc tcccgctgtt gacgcgccag ctatggaagg
     6361 ctgccggcct cttagggacc ttcctcttcg cctgcatggc ctgtgaactc atcagcctga
     6421 cgacgattcc ctcgtcgaca gcggccttcc tggaaaatac atccgccgtc tgggtcctga
     6481 tcctaatggc cgtcgtcagc caccattggc cgggaaagca agtcctgacg gctacgagcc
     6541 tgctcctcat cggcatcgcc tgtcttaccc tgcaaggcgc cgttttccat ctggcaccgg
     6601 gagaaatcat ctgcctgacc ggttccgtct tctacgccct ttggatctgc ctgaccgccc
     6661 gcctggcccg gcagtacgac ccattgctgc tggggatctt gcaaatggga ttcctcgccg
     6721 tgtacagtac agcaggcatg gtcttatttg aaagcccggc cgttcctgcc gatacgacga
     6781 cgtggggtgc catcctggcc ctggtcttca tctgttccgt ctttggcttc accttccagc
     6841 ccgtcgccca aaagtatacc agcgccgaaa aagccggcct gtttacagca ctgaacccgc
     6901 tcatcgccgc cgtcctgggc gtcattttcc tgggcgaaac ctttacgccc gtccaattag
     6961 ccggagccat ctgtatcctc ctgggcatca tcatcgtcca gcttcccgaa aaatatcgct
     7021 gcaaaacgca tagctgtcaa aatggatgac acctgccttg acaaaacacc tccgcccggc
     7081 ctatgctgaa tgcggaggtg tctttatgaa aaaaaatcgt cttatcgcag ccggactctt
     7141 aggcctattg tggcttcccg gcctggcact gggccaggaa gaggatgtac aacaagccaa
     7201 gccagcaacg gtatggcagg acctgtatta cagtcgccaa gaaaaaaaag acctcatcat
     7261 cagtgctgat gacaaagtcc ctcattcttt ctacggcctg cagattggcg acaaaaaaag
     7321 cgacgccctg aaaaagctgc gtccagcctt caaattcatg tatgaactca aggccatcgg
     7381 taacgaatac ctcctgtccg acgtcgtcta cccggactat tacctgacca tcatgttcga
     7441 cgaaaagggc cgggtattca tgataagcta tcaaaaaaac caaaaatacg aattgctgta
     7501 gaacggcgtt caaagaaaac attattcgca ggccgccgat cgcagttagc agacggtcca
     7561 ccggaccgct ttcgtagggg acatcctgta agccttttgg gcccgtcagg tgccgaccag
     7621 caggaggcgc aggacggctg ccaaaacgga cagctgtggc taggcgtccc gttgtgggaa
     7681 tcccgcgaaa tgctgttcgg agaaaacgcc attcgcgggc cgccggtcgc agttcgtaaa
     7741 accatgcctc atggacctgt tatgtatatc accatttatt cgcggcgggc cgcccaaggc
     7801 tccctttata ggggagctga ccgctaaagg cggtctgaga ggttaaagat cggcccctac
     7861 gcaggccccg gtcggggcgt ttttacgagc tgagggttac tcgaagcgat gttgttagtt
     7921 tgagtttatc gctgcgcagc cgcatcgtag ggg
//
LOCUS       DAWABZ010000082         7813 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2513_length_7813_cov_3.577468, whole genome shotgun sequence.
ACCESSION   DAWABZ010000082 DAWABZ010000000
VERSION     DAWABZ010000082.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7813)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7813)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7813
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2513_length_7813_cov_3.577468"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    924..933
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1608 a         2040 c         2268 g         1887 t
ORIGIN      
        1 ggtaaaaaat ttaaaaccag cttctacaaa ctacaaactc catactacaa actataagtt
       61 attccatatc ataatcctgc aaggcagcga cgtggtgata gccttcgttt ttgcctttgt
      121 tggcaaagac gcggaggact tcacgggctg tatcgaggga ggtcaggcag ggaacggcaa
      181 gttctacggc catgcggcgg aggttgaagc cgtcgcgttc cgggtgtttg ccataggtca
      241 gggtgttgag gaccaagtcg attttgccgt tgcggatcag ggtggcgata ttctggccgc
      301 cttcgtggat cttgcggacg atgtcgacgg gcatgccgtg gtcgttgaag tatttgcctg
      361 tcccttcggt ggcgatgatg tgatagccca attccttgaa gccttcagcc agctggaggg
      421 cttcagcttt atcgcggtcg gcgacggtga cgaggatcgt accgccagcg gggatcttca
      481 tcttagcgcc attgacggct ttgtacaggg cttcggagta cgaatggccg atacccatga
      541 cttcgccagt cgatttcatt tccggcccga gggcgatttc agccaggccg atcttactga
      601 acgagaagac tggagccttg gcggcgacgt gtttcttggc cggcatgaga ccgctgtgat
      661 agcccatgtc cttcaaagtt tcgccgaggg cgatgcgggt tgcgatttcg acaatcggga
      721 aatgggtgac cttgctcagg aagggaacag tacggctcga acgcgggttg acttcgatga
      781 tgtacaactt gccgcgggaa acgacgaact ggatgttgac cgaacctttg acctggagtt
      841 ccagggaaat gcgcgtcgtg tagtcgacaa tctggttgat catatcctgg tccaagtgct
      901 gcggcggata gacggcgatg gagnnnnnnn nnncctgttc catgatgccc gggatgtaga
      961 cgtcagtacc gtcggcaacg gcgtcgactt cgacttcacg gccgaccatg tattcgtcga
     1021 tgaggatcgg gtggtccggt gaagcgatga cggcttcacg gagatagcgt tccaattctt
     1081 tttcgttata gacgatttcc atagcccggc cgccgaggac gtaagacggg cggacgatga
     1141 gcgggaattc caattccttg gaaaccttca tggcttcttt atcactggta gcgatgcggc
     1201 cgtccggacg gggaatgttc aatttggcca ggacttcgtc gaagcgttcg cggtcttcgg
     1261 cgcggtccat gccgtcaaca gacgtaccga ggacggtaac gccccgtttg gccagtggag
     1321 ctgccaggtt gatagccgtc tggccgccga actggcagat gacgccgacc ggtttttcct
     1381 tgtcgacgac agccatgacg tcttcgacgg taagcggttc aaagtacagg ctgtccgacg
     1441 tatcgaagtc ggtgctgacc gtttccgggt tgttgttgac gatgatgctg ttcatgccgg
     1501 ctttgcgcag ggcccaggcc gaatggaccg agcagtagtc gaattcgata ccctgaccga
     1561 tgcggatcgg gccggaaccg aggacgatga cgctcttctt gcccaggggc ttgacttcgt
     1621 cttcgtcggc atagcacgaa tagtaatacg gcgtagcggc ttcaaattcg gcagcgcacg
     1681 tatcgaccat cttgaaggtc ggcgtaatgc cgtggtcgag gcggaactgg cggacttcgt
     1741 cttccgtctt gttggtgaag ccggcgatga cggcatccgg catgctgtag cgtttagcca
     1801 ggcggatgtt atcgaccgtc aggttgttgc gcttcaagtc gaattccaag tcgatgatgt
     1861 tcttgatctt atggaggaac cacaggtcat agcccgtgat gtaatgaatc ttttcttcgc
     1921 tgatgccgag gcgcagggcc tgagcgacga cgaagagccg ttcatcatcc tgtttctgca
     1981 gaagttcgac gatgtcgatg agggacttgc tttcgagctt cttcatggaa atatagttga
     2041 cgccgatttc cagagagcgg acggctttga gcagggcccc ttcaaagcag cggtcgaggg
     2101 ccatgacttc gcccgtagcc ttcatctgcg tgcccagggt cttgtcggcg agggcgaatt
     2161 tttcaaaagg ccagcgcggg aatttgacga cgcaatagtc gagggtcggt tcgaagcagg
     2221 ccgtcgtctt gcccgtaacg gcattggtaa tctggtccag ggtcaggcct gtagcgactt
     2281 tggcggcaac tttggcgata ggatagcccg tcgctttgga agccagggcc gacgaacggc
     2341 tgacacgggg attgacttcg atgacgtaat actggttgct gttcgggtcc agggcgtact
     2401 ggacgttgca gccgccttcg atcttcaagc ggcggatgat gtccagcgat gccgtacgca
     2461 gcatctggta ctgcaagtcg ttcagggtct ggctcggtgc gacgacgatg gagtcgcccg
     2521 tatggatgcc gactgggtcg atgttttcca tgttgcagac gatgatgcag ttgtcggcct
     2581 tatcgcgcat gacttcgtat tcgatttcct tccagccggc aacggagcgt tcgacgagga
     2641 tctggttgat cgggctgagt ttgatgccgc gcgtggcgat catgaacaat tcgtcttcgt
     2701 cgtgggcgat gccgccgccg gtaccgccaa gggtaaaggc cggacggacg atgatcggat
     2761 agccgatttt gtcggcgaat tcgatggccg acgggacgtc ttcaaagata tcgctttccg
     2821 gaacaggctg gttgatatct ttcatggctt tcttgaagag tgcccggtct tcggcttctt
     2881 cgatagccga cagatgcgtc ccgaggagct ggacgttata tttttccagg acaccggcgt
     2941 tagccagttc gacagccata ttcaggccga cctggccgcc aagggtagcc agcaggccgt
     3001 caggccgttc tttttcaatg acttccgtca cgtagtcgag ggaaatcggt tcgacataga
     3061 cacggtcggc aatgtcttcg tcggtcatga tcgtcgccgg gttgctgttg atgaggacga
     3121 cttccagtcc ttcttccttc aaggcgcggc aggcctgagt gccggcatag tcgaattcag
     3181 cggcctgacc gatgataata gggcccgaac cgataacgag tactttcttc aatccttcta
     3241 tcatgttagt tttcctccat catcttgacg aattgtgtaa acagatacaa attatcagtc
     3301 ggtcccggcg aagcttccgg atggtactgg acgcacatga tcttcttgga cgggacttca
     3361 aaaccttcga gggtgttgtc gtggaggttg cgatgggtga tgacgacgtc atcgggcaga
     3421 gaggcttcgt cgatgacata gccgtggttc tgggacgtga tgtagacgcg gcccgtgcgg
     3481 atatccttga ccggatggtt ggcaccgcgg tggccgaagg tcagcttgcg gctcttgccg
     3541 cccagagcca ggccgagcat ctgaaggccc aggcagatgc cgaaaatcgg tttcttgccg
     3601 atcatcttct tgacttcttc gacgatgtac gggacgtctt tcgggtcgcc agggccgttg
     3661 ctcaggaaga taccgtccgg attgacggcc aggacttctt cagccttggt ttcagccggg
     3721 aaaatggtca ggcggcagtc gctgttgacc agttcgcgca ggatgttggc tttggcgccg
     3781 taatccatga cggcgacgtg gaagcggcca tcgcccatgt gcttgatttc acgcgtcgtg
     3841 acgcggcgga cgagctgcgt ttccagcggc gtatcgaaga gttccttgat agcgctttct
     3901 tccatcgttt ccggcacgat gacgcctttc atgacgccct ggctgcggat ggcccgcgta
     3961 atggcacgcg tatcgacgcc gtagaggcag gggatgtggt gtgtcataat aaaggtagac
     4021 agcttgcctt cgttctgcca gttgctcggt tcttcacaga gctcgctgac gatgaagccg
     4081 gcagcatagg gacggtcggc ctgttcgatt tcgtggaaca aaccgtagtt accgatgagc
     4141 gggtaggtca tggtaatgat ctgaccggca taggacggat cggtgaacgt ttcctgatag
     4201 cctgtcatgc ccgtattgaa gacaacttcg ccgacagctg gttcatccat cagcatgttg
     4261 ccataaaact tctgtccatt ctctaatatg aggacacctt tcattataaa acctccccgt
     4321 ttttcattac aatttcacca ttgacgatgg tagctgcggc cttgcctttg aaaatcattt
     4381 catcgaaagg cgacgctttt cccttagaat agaatttcga ggaatctacc gtccattctt
     4441 tatcgagatc gagtacggtc aaatcggcat ccttgcccgg tgcgatgaca ccggcatcga
     4501 ggccgaggat cttggcctgt gccgtcgaca tggcattgac cagagtcatg aggtcgatat
     4561 gacccgtatg atacatataa gtcagcatgg cgccgacgct cgtttccaag ccgacgaagc
     4621 cgctgggagc caggttgaat tcctggtcct tttcttccca ttcatgcggt gcgtggtcgg
     4681 tgacgacggc atcgatggtg ccatcctgca ggccggcgac gcaggctgcg cggtgttctt
     4741 ccgagcggat cggcgggttg actttatacc gtgtttcaaa agtgcgcagg gcttcatcgg
     4801 tcaggatgag gtgctgcggc gtgacttctg ccgtgacctg gacgccgcgc ttcttggcct
     4861 gacggaccag ttcgacagcg ttcttggtgc tgatgtgggc gatgtggacc ggcgtgtgtg
     4921 tcgcttcggc caggaaaata tcgcgggcga cggcaatgtc ttcagcgaca gccggacggc
     4981 ctttgatacc cagttcgttg gaaacgacgc cttcatgcat gttgccgcct tcgacgaggc
     5041 tctgttcttc gcagtggtcg atgatgacct tgtggaacat gctggcatat tccatggctt
     5101 tgcgcatgaa atcgcagttt tcgacgtaac ggccgtcatc ggagaaagcg gcgacgcctt
     5161 ctttggccat ggcacccata gcggccagtt cctgaccctg gaggcccttg gagatggcgc
     5221 cgatgatttc gactttgact ttggcttccc gttcgatctt atacttcatg ccgtcgacga
     5281 tgattgccga atcgatgacc ggcttggtat tcggcatggt cgcaacgcgg gtgatgccgc
     5341 cggcagcagc tgccttcgtc cccgtttcaa tcgtttcctt gccggactgg ccgggctggc
     5401 ggagatggac gtgcatatcg atgaagcccg gtgctacgac gaggcccgat gcgtcgaaga
     5461 tttcggcgcc gtcagcgctc agattggctc cgacttcctt aattttacca ttttcaatga
     5521 gaatgtcaca aatttcatcc tgctgcatgg ccggatttac gactctgccg cctttaacga
     5581 gtagtgtcaa cgtcatcgcc tcccataatt gtcagatata atacggccat acggacggat
     5641 acgccgttct tgacctgttc ctgaatcgaa gaaaattcac tgtaacatgt atccggttcg
     5701 atttcgatgc cccggttcat gggacccgga tgaaggacga ggacgtcgtc tttagcccac
     5761 ttgaggcggt cgcggttgat accgaagagc gaattgtatt caccggcgct gggtaagaag
     5821 ccgatgccct ggcgttcttt ctggatgcgc aggacgttga tgacgtctgc atcctggatg
     5881 gcttccttca agtcgtagtg gactttgacg cccatggctt ccaattccgg ctggagcagt
     5941 gtccgcggac cgacgaggtg gacgtcagca cccatggtct tgaggccgta aatattggag
     6001 cgggcgacgc ggccgtggtc gatatcgccg acgatgacga ccttcaagcc gtcgatatgg
     6061 cctttgactt cctgaatggt gaacatgtcg aggaggccct gcgtcggatg ttcgtgggcg
     6121 ccatcaccgg cgttgatgat gatcgggtcg acgcagcggt cggcaaacca aggcgaacct
     6181 tcctgtttct gacgcatgac gatggcatcg acacccatgg ccgttaccgt cagcagggta
     6241 tcgcggaaac tttcgccttt ctggatggag cttccccgcg gcgtgaagtt gatgacatcg
     6301 gcgccgagat acttggcagc catttcaaag gaaccgcgcg tacgcgtgct cggttcccag
     6361 aacatgttga ccaccgattt ccctttgagc agggataact tcttattccc gctgttgacg
     6421 acggctttca tcttcttagc tacctgcagt atcaactcaa tttcttcttt cggtactccc
     6481 tggagcccca acaggctttt gccctgtata ctaaccatcg aatcctcctt gtaaaaaaag
     6541 acctctgccg cagggcaaag gtcatattat aagcatggca aaatatgtcc ctttttagcc
     6601 tctccggact atattaaagg ttcaatctga aagtattaga atcgcacagc ctcacgggac
     6661 tgccacatat tcttctatat attgtatcgg ccagggccta taaagtcaag cagttttttc
     6721 acgacttcaa tccgtcgacg ccggcgtaat ggtcacggcc tggggcgtaa tggtgacgct
     6781 gttgtaaaag gcttcaaaga gctgggccgg gacgtacatc gtaccgttga cattgaccgg
     6841 gccggcaccg tactggaaga tgtggttcaa gttgaccttc ttgattgtcc cgatccgttc
     6901 gtaccagtct tcaccgggtt tgacgatgat ataggcgata ttcatctcga cgcggacgct
     6961 ctggtcgtca ggattccagg tcacggtata accgagctgt tccgccactt tacgcagagg
     7021 aatcatgacc acgttatttt cctgtaatac ggctccatcg gccgggtcca tgaccttgcc
     7081 ggcaacggtc agctggcact gctggatcgc cgtcagcccc ggaatcgacg tatacggccc
     7141 cataaccggc ccttcatcat aggcccaggc cggcccgcaa gccaaagccg ccacgaccat
     7201 ggctaagata atactcttct tcataaggga ttcccccttt atccaaaaca caaaaatgag
     7261 tcataaataa ggacatatat aatatattcg acaaatcccg ccgttttcct gctaatattt
     7321 gaaagaacgc aggtcgcccc tggctcccct ataaagggag ccttgcaaaa aaagcgctgc
     7381 catagaagac agcgcttttt tgcaaaacta aaaatcaaag aatcccgtct ggtcggccat
     7441 gggtacgacg gggaatcccg tcggtactgg ctggatgatc atgaacacca gggctgccag
     7501 gacggcaatg cccagggtga ggtacgagaa gcttcccttt tccttatgtt catacaggcc
     7561 gaacaaacgg atgccgacag ccgaaccgat ggtagcgaac aaggcaccca gcgagaaata
     7621 ctggtacatg gcatacgatg ccagcagggc aatgatcatg gccccgttga tgacgatggg
     7681 atagcattta gacaagaccg attcgggatt acggcgcatg gccgtgagcc agtccgtccc
     7741 gtcgatgccg ctcaggcccg tgaggaacgt cgaagcgtag agcagcttat cccaccaggc
     7801 cgttcccttg cgg
//
LOCUS       DAWABZ010000083         7787 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2525_length_7787_cov_3.341956, whole genome shotgun sequence.
ACCESSION   DAWABZ010000083 DAWABZ010000000
VERSION     DAWABZ010000083.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7787)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7787)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7787
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2525_length_7787_cov_3.341956"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1864 a         2133 c         1873 g         1917 t
ORIGIN      
        1 tgcggacgaa tatatctttt gcatattggc tcattgggtc agaaaatgct ttgcgtcggt
       61 acttcaccac taaaacaaga tggtaataga gtaagaacac tgaatggtta ttactgtcta
      121 attccatagt ataatcagtc ctttctctct tactactgat tatactgcaa aacaaattat
      181 gaagaaattg tctttctgag ggcatcgaac cctcagaaaa acctgcaatt catctcaccg
      241 cctaaagagg caggaaactt cttgcagaat caggttaaac tttattatac catatttatg
      301 tccctgcgag aatcagcagg caggacagaa tcgccgcaat ctgtcttatt tacatggtcc
      361 atatcttggg ataaaataat agtacagttt acttttagga ggtttttcta tggcaactaa
      421 acgcgtcctc gcaatccacg atatgtgttc tttcggccgc tgctccctga cagccgccat
      481 cccggtcatt tcggccatgg gcttccaggt ctgccccttc ccgacggcta ttttcagcaa
      541 caacctgacg tatggcgact ataccttctc tgattttacg ccgcacatga cggaattcat
      601 ggataaatgg cagaaactgg gctattcgta tgacgccatt tacagcggct tcctcgccga
      661 cgccggccag atcgccatcg tcctcgacgc catcgcccgc ttcgccaaga aggataccct
      721 cgtcgtcgtc gatccggcca tggccgatga cggcaagctc tacccggtct tcaagcccga
      781 tattgtcatg gaaatgcgca agcttatcgc caaagcgacc atcattacac cgaattatac
      841 cgaagctgcc ctgctggccg acatccccta tgcccagggc gcgccatcca tgaagactat
      901 gctcgaactc tgccagaagc tggccgccat gggaccgcgt caaatcatca tcaccagtgt
      961 ccccacgggc aacgaaacga tcaccgtcgt cagctacgac gcccagacga cgtctttcga
     1021 cgaatgtacc gtcgaccgca tccccttcag cacctgcggc acaggcgacc tcttcaccag
     1081 cgtcctcaca ggttccctgc tctagggcca gtccctgcac gacagcgtca aaaaggccac
     1141 acagttcctg aactacgcca tcggctacac ctaccagtcc ggcagcgatc cccgcgaagg
     1201 cgttcaggtc gaaccgtgcc tgaaaaagct gttctaagtt ttattgttaa cttatataaa
     1261 tccatataaa gttgacctca tgagcgcttt tttgctataa tacggaagaa gtccgtaaga
     1321 aaaggagcgc ttttatcatg tctgattcaa gagaaaagac gatgcgcctg acgcgcatcg
     1381 ccttattgat tgctatgaac tgtatttctg cctatttgat cattcccctg cccttttccc
     1441 agtcgcccat cgccttgcag acgctcgtcg tcaacctggt cgccttcctg ctgccgccga
     1501 aagatgcagc catcaccatg ctggcctaca tcggcatcgg catcatcggc gtccccgtct
     1561 tcacaggcgg tacggcaggt cccggcaaga tgttcggccc gacaggcggc tatatctggg
     1621 cctatgtcgc cgccgtcttc ctcatgtcct ggttcaaagg gaaggaatac aacttccgtc
     1681 gctactctct ggcagccgtc gccatcggta tccccgttat ctatatcggc ggcgtactgc
     1741 agctcaaagg catcacggga atgccctggg aagcagccat catgagcggc gtcgtcccct
     1801 tcatcccctt ggacatcgtc aagagcctct gcgctgccgc tctggcacgg cccttattgg
     1861 ccataaataa aaccgtatct cacgaatgac gccagcttgt gtaaatccgt tctttgtcag
     1921 tatacatctc ccgatctgcc tgggataaca cctcgtcgat attggcaata ggcgtctggc
     1981 agacgacaac gcctaaggcc atgctgatgc cgctggaacg gtaacgggcc agcagctcgc
     2041 ggacgagctg ctgagcctgc tgttcactgg cattcctggc gatgaccatg aattcatcgc
     2101 cgcccatgcg gaatacggcg ttatcgccgc tcaaggcttt catatgccgg acagcctgct
     2161 ggaggagatg gtctccggct tcatggcctt gcgtatcatt gattcgcttg aggccgttga
     2221 ggtcgccaaa aataaaggcc aggaaaatcc catcgggcac agcccggata tattcggtaa
     2281 acccccggcg attgccggcg ccggtcagct ggtccgtcgt gctgaggcgt tccagcgaac
     2341 ggaaagtatc gcggttgcgc agcatactgg ccaggaacaa ggtcagcgtc gacaacagga
     2401 ggctggccga tttaaaggcc aatgccgacg ggttgacgac ttccgtaaag cccagggact
     2461 tgcccgattg agtcagatgt cccgatacga ggcggcggac gccggaaaga tgcggcgtaa
     2521 atccaggatg agcccggacg aaagcctgtg catcttcaat aatggccatc tgtttagcat
     2581 caaaggccgc atacagcggt cgcagggagt ccatgggaat gtgctggacc gtaggagcca
     2641 tcggctgcaa atcctcttga taccattcat aggtagcgct gacggtctgg tccggctgtt
     2701 cctcaaaaat caggacccgt tcagcttgca ggctatgacc gattttttcc agcattttct
     2761 gcaggcctgt atggggatcg gcttcacgca tgccgatggc aatgacgtcg ttggccatga
     2821 cttcccggaa gacgacatca atttcttccc ggtcgcggaa aatataccgg ctcaggtcga
     2881 aagccataga aaaacggtaa ttcttcccct gccaaggaat gagcgtcgtc cgcatgaaca
     2941 ggtattgccc tgtcttgcgg ttgcggcgcg ttgccgtata aaaacaatcg cggcgcagcc
     3001 tgtcattgag gcaattatcg cagggagtct cccggtcagc caggatttta taacatttct
     3061 caccgaccca gctgctgcta tcagaaaggc cataggcccg ttgcatatag tcattgacat
     3121 agaccagttc atacgtatct gggtccatga cagacacttc ttcatcgagc ttgtcgaatt
     3181 tctccttgtc gaagaccagg cggtcgccaa tatagatggc ttgcccatac tgctgttctt
     3241 tcgcttcatc cagtcgtttc cgcaacagct gcatgacctc atcgaaatcc tggccctccg
     3301 acccctgggc cacggcgtat tgcaaataaa gagtacaact gtagccgcca atattcgtaa
     3361 tgccatggat agcattgatg aggcccagca ttttattccg gaaatcggca tccatcgcct
     3421 tcgggacaag gcagcaaaaa cggctgccgc tcaaatgggc tatcgtttca ttcaacacat
     3481 ggaaggccag cagctgctgc gtaatgacct gcaacaattt ctgtcctact tcatcgccat
     3541 actcctgaaa aagcgcttcg tatgcaggca cttcgagtag gacacacgta taatcctgtc
     3601 catagcgccg atatttatcg gcatactcgt ctgccgcctg caagataccc cggaaattga
     3661 gcagccccgt cacatcgtcg acaaaactgc tctgccggag ctgcacctgc cgggcttctt
     3721 catactctaa atcctcaaaa taacccatca ggccgataat acgccgtccc tggtagatag
     3781 ggaatttcgt agctgcgatt cgatgcggtc ggccttggac gagacactcc ccgataatat
     3841 gtcgcgtaat ttcgcctttt tccaggacag cccgttcggc ttcctcatac tttttcccct
     3901 ggatatgcca gccaatctcc tcatcggtct tcccgatgag gattttttca tcggtcaggc
     3961 cataatagtc gaggaaggac tgactgacgc cggcaaaacg ccgttcccgg tctttccaga
     4021 agaatttgat atccgaataa tgaatcatgg cctggcagag ttcccgcaag aggcggaaat
     4081 aaggctgccg ggccggttcc aattcgtgga tgccaaaaga agtggccaat atgggcaaca
     4141 ggctcttgcg gtcccgcgcc gtttcccatg catcatcgcg gcagcacaac agaatgtcat
     4201 attggccatc ggcctgctgg aggcggaggg ccaaaaagac agtccagcga taactgccgt
     4261 cggcctgttt gatacggaaa acgtcaccga cactggccgt cggggactgg tccgcctggt
     4321 ggcaaaggtt atcgaaatca aggaatgata agaacctttc cctgtcgtcg acgtagacgg
     4381 actggacagc atagtcgatg agcagctttt ggatgccttt cagggacttt ccatcagccc
     4441 aatgggtcat cgtcgtcttg atgacttcaa acgtatcttt gccaggatgc aggtaatata
     4501 agcctttata gaccttcagg atattgcgca gcagataatc gatacgctgt atctcctgac
     4561 gttcgctggc atcggcaata ttatagatgc aggcccgtcc caggtatcgc ccctgagttc
     4621 caccgatgag ttccaaatgc agctgcaaat aatggtctct ttcaacatag gtcatgactt
     4681 gattttgccc ggaaacaagg agttcatcca gacaaggttg caggcgctgc ctgaatgaaa
     4741 agtcctgaat ccacaagggc aggtcatggc cattgtatcc gggcaaagac catcggagga
     4801 tttttcgaaa agccagatta gcccataaca ccccggcctg agtgccatcg tagtgaaaaa
     4861 tagcagtcgg taaatccgtt acgacattga ccagaccggc tttatcatag actggttctt
     4921 cccgccgcgt ttccaagcca tgctcatagt gcttgcaaaa cactgacaag tcttcgtagg
     4981 tcataggccg tccaaaataa tagccctgta tcttttcaca gccgacatcc ttcaaaaagt
     5041 cgacgtgggc ctttgtttct gcgccttcgg ccaaagtatg tatgcccagt tctttcgcca
     5101 tgaggacgat ggccttgata atctgacggc tctgttcatt gaaattatgt aaaaaggcca
     5161 tgtcaatctt cagttcatca aaatgatagt tgtgaaggac gttgagcgag gaataggcac
     5221 tgccgaagtc atcgagccag acttggtagc ccgactactg gaactgcttc aaactgcgga
     5281 gcagattgtg cttttctttg accaacgccg tttccgtcac ttcgatgcgg aaataatccc
     5341 gctgtagatt atagcgggaa atgacttttt caacaaccgc aaaagggtcc atgaggttga
     5401 aatcatgacg cgataaattg accgataccg gcaggacagg ccttttattg gccagctggt
     5461 aatgaagctg cttcgctacc tgattgagga catagccatc cagcttatgg atgagccggg
     5521 cctgttccag gacgggaatg aagacattcg gcgtgagtcg gccgtaaacg ggatcttccc
     5581 agcgggccag cgcttcgaca ccgcagattt tcccggtcat cgtacggacg acgggttgat
     5641 aatagatttt gatgtagcct ttttctaagg ccgtgttgaa attttccagg acgaaatccc
     5701 gcttctccag ggcgacgccc atttcccgcg tataaatagc ccagcaccga gtggcatcag
     5761 tcttgatggt atcacaggca atcttggcca tgtcgaaagt atggcgcacg atatggatat
     5821 cgtcctcttc gagcaggcag acgccggcct tcaagacgat attggaatta ttgatatacg
     5881 cgttgacacg gctgcagatg acttctatct tttggaatat atcttcccgc ttcgccagga
     5941 cgccaaaacc atcggccgaa aaatgggaaa tgaggtctcc cgggaaatac tggcgcagca
     6001 gctcggcaat gcgcttgagg caatggtcgc cggcagccag tccgtgtagg gaattgtaga
     6061 ggcggaagtt cgtcagattg aaataagtcg gacagtattc agacagacgg ccttggggac
     6121 tttcccgccg ggctacctgc agggctttct tgaagaacag gcgcatcccc atcagccccg
     6181 tcagcgggtc gttctgggta ctgctgcccc tcagttcact gctgatgagc tgcagcagcc
     6241 atgccttggt cccatcttcc agagaaatga cgctggccga accttcggcc cggcggaaat
     6301 ggtcatcccg cgtgcggaag cggaaggtca cgaactccga atagtccgtt tccgacatgt
     6361 gggcgacagg ataatagtcc tcactatcga ccatgccccg gaaccgccgt cccgtaaagc
     6421 ggtaaaactc cgcttcatcc atacaatgat aaatcttcag catcccttta ttgacgaata
     6481 aaatgggttc ccccggttgc ataccgatca ggcagatacc ggacgtcagc cggtcgtaat
     6541 agcgatgcag ctcgctctgg atttcctcta gccccatgcc cttcacatcc ttctatgcca
     6601 tgcgttataa agcattatga ttgtcaagac aaattatata aatagcgttt acaaaatata
     6661 cgttcattat atcacaaggc agtcattcac gcacgggttt ccggtctgtc cggtataaca
     6721 ggatatagcc aaattcgaca gccaccagca tcagtcccca ggcagcgctc gcccagttca
     6781 atgtatgcag atcctgccgc agtgaaggcc ctgtcaccac gtaagcagcg gcacacagga
     6841 aaaactgacc agataggtaa cactgctatc aactcctttt ccatgtggat gtgaggacaa
     6901 tattcatcca gatattcata atcctgccca ctttagcagc ggggaaccag accatcctcc
     6961 cgacttttct gcctgttatt tcaaggcctg ttcggtatct gccagaaaca gctgcacaca
     7021 atcggaaaaa tccaaccccg cccggtcggc cagaacggcc agccaccata cgcattcccc
     7081 gattttagac gggagtttgc cttcttcatc agatggccag cgcccttcat gactcatagt
     7141 cagccgtcct accagtccgg cgtcggtcaa aaaagccagg gcatcttcct ctgccgacca
     7201 ttcggagcca tgatattccc gttccagagc atgataggcc tttctgatgg ccgcagagcg
     7261 tttcgatact tcttgtaatt ccatatgatt ctctcctttt tgagtttggt cagtttggtt
     7321 agttgtttag tcagtttggt tagttatttg gtcagttttc agagtttggt cagtcgttcg
     7381 gtcagcagaa tggataaaag cgatacctgt cccctgacgg tgccatgacg gaatagcatt
     7441 agttgaatct cacgacccag tatcctttcc ggtgcgttcc catacgttga agacagtctt
     7501 tttcctggag ctttttcata taatacttta tcgtactaag agacagtgat gttgctttag
     7561 cgatttcatg ttgcgtgcca tccggtttct tttgaagata tgacaaaata actttttcct
     7621 tttcatttag ttttacaggc atattcgttc ccgtttgtcc agttttcggg gtttggtcat
     7681 tttttttagt ttggtcagtc gtttggtcag ttgtccggta caagttaacg cgcaaatcgc
     7741 catcaaaatc aatgatttcc ggttccttca aaccataatc ccggcaa
//
LOCUS       DAWABZ010000084         7744 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2542_length_7744_cov_3.417349, whole genome shotgun sequence.
ACCESSION   DAWABZ010000084 DAWABZ010000000
VERSION     DAWABZ010000084.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7744)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7744)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7744
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2542_length_7744_cov_3.417349"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    687..696
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1375..1384
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1848 a         2326 c         2124 g         1426 t
ORIGIN      
        1 ccgcaacggc acagccgaaa aagaagaaag gacccgctca cggtctggac cggagaacct
       61 gggtcttgta cctcgtcggc ttttccgccc tggcctggtc ctattttatc ttattcaccc
      121 gcaactggtc ggacgcaggc tttgccttca ccgtcctggt tacggttctc tggccctggt
      181 ctttgtataa gaattggaag aaagggccga aaaagaggta acgcttatgg atatgtccca
      241 ttacaataaa gattacgata cgtcccaccc tttccacgtc gtcgcccctt atgaacctat
      301 gggcgaccag cccgaggcca tcgcctctct ggcagacggc atccgttccg gccaatgggc
      361 ccaggtcctc gtcggggcta ccgggacggg caagaccttc actatggcca agctcatcga
      421 agccgtccag aaaccgaccc tggtcattgc ccacaataag accctggctg cccagctgtg
      481 cagtgaattc aagtcattct ttcccgataa cgaagtcggt tatttcgtca gttattacga
      541 cttctatcag cccgaagcct atatcgcctc gacagatacg tacatcgaga aagacgcgtc
      601 catcaacgat gaaatcgata aattgcgcca ctcggcgacg atgagcctct ttgaacggcg
      661 cgatgtcatc atcgtcgctt ccgtttnnnn nnnnnnatct acggcttagg cgacccggaa
      721 gactacagtg aactcgtcgt atccctgcga ctggggcagg aaaagccgca ggaagaaata
      781 ctgcacaagc tcgtcgatat ccagtacagc cgcaacgaca tgaacctcga acgcgggacc
      841 tttcgcgtcc acggcgatac aatcgaagtc tttccggcgg ccttcgataa ctccatcatc
      901 cgcatcgaaa tgttcggcga tgaaatcgac aggctcacgg aagtcgacgt cctgacaggc
      961 gaagtcattg ccgagcgcaa gcacgtcgcc atttatccgg ccagccatta tgtcacgacc
     1021 agggacaaga tggaacgggc cctggggacg atccgccagg aattggacga acggctgaaa
     1081 gtgctgaaag acggcggcca tttgctggaa gctcagcggc tggaacagcg cacgcgctat
     1141 gacatggaaa tgatggaaga aatgggctac tgctcaggca tcgaaaatta ttcgcgccac
     1201 ctggccggcc gcaagcccgg tcaggcgccg tatacgctcc tcgactattt ccccgatgac
     1261 ttcctcatcc tcgtcgacga atcgcatgtc accctgccgc agctgcgggc tatgtacgcc
     1321 ggcgaccggt cgcgcaagga aaacctcatc gaatacggct tccgcctgcc gagcnnnnnn
     1381 nnnncgatta cgcccgcaaa aaaagccttg tggacttcgg cttccgcctg ccgtcggctt
     1441 tggataacag gccgttgaaa tttgaggaat tcgtcgaacg catcaaccag attgtctacg
     1501 tcagcgctac gcctggccct tatgaaatgg aagtccagac caatctggcc cagcagatca
     1561 tcaggccgac gggcctgttg gatccgaaag tggaagtccg tcccattgcc ggccagatgg
     1621 atgacctatt gggtgaaatc cgcaagcgca cggaaaagga cgaacgcgtc ctggtgacga
     1681 cgctgaccaa gaagatggct gaagacctga cggattacct gaaggaaatg ggcgtccgcg
     1741 tgcgctatct ccattcggat atcgccacca tcgaacgggc cgacatcatc cgcgacctgc
     1801 gggccggcgt cttcgacgtc ctggtcggca tcaacctcct gcgcgaaggc ctggatatgc
     1861 cggaagtctc gctggtagcc attctcgatg ccgataaaga aggcttcctg cgttcagaaa
     1921 cgtcgcttat ccagaccatg ggccgggcgg cgcgaaacgt ccatggacag gtcatcatgt
     1981 atgccgacaa gattaccgac tccatggcca aggctatgga agaaacgcag cggcgccggg
     2041 aaatccagga tgcatataat aaaaaacatc acattacgcc gaaatcggtc aaaaaagacg
     2101 tcgtcgacct catcgaactg acgaaagtcg ccgaaacgga agcggcctat ggcagccaga
     2161 agggaaggaa aggcggcaag atgacccgcg aggccctgaa caaagtcatc aagaccatga
     2221 ctttgcagat gaaagaggcg tcgaagaacc tggaatttga aaaagccgcc gaactgcgcg
     2281 accgcctggg ccaattgcgc cagcagctgg ccgatatgag ccacgatgat accctgccta
     2341 aagacatgca gggcgaagac atgcccaagc gccgctaccg ccgcaagata aagcggcctg
     2401 atgcgaagaa gaagtaaacg tagttcgtag ttcgtgggtc gttcctcgaa gcaggtttta
     2461 aattttagcc catcacgacg aacgatgaac gacaaacgat gaacgttatg ataacgggag
     2521 gcacgaagtg caagataaat atattgtcat caaaggtgca cgtcagcaca atttaaagaa
     2581 tatcgacgtc aagatccccc gggacaagct cgtcgtcttt acgggtctgt ccggctcggg
     2641 caagtcgtcc ctggcttttg atacgattta tgccgaaggc cagcgccgct atgtcgaatc
     2701 gttgtcggcc tatgcccggc agttcctggg ccagatggat aagcccgacg tcgattacat
     2761 cgaaggcttg tcgccggcca tttccatcga ccagaagacg acgagccgca atccccggtc
     2821 gacagtaggg acggtcacgg aaatctacga ttacctgcgt ctgctctacg cccgtgtcgg
     2881 cgaggcctac tgcccgaaat gcggcaagcc catccgtcag cagaccatcg agcagatggc
     2941 cgatgccgtc atggccctcg gcgaaggcga gaaggtcatg gtcatggcgc ctgtcgtcga
     3001 cgggcgaaag gggactcatc agaagctcct ggcccggctg gtcaaagacg gctatgtccg
     3061 cgtccgcgtc gacggcgaag tttatctctt gtccgacgat atccccctgg ataaaaataa
     3121 aaagcacact atcgacgtca tcatcgaccg cctggtcatc aagaacagta tcttgacgcg
     3181 cctgacggat tccctggaaa cggcggctaa attggccgat ggcatcgtcc atattttccg
     3241 catggcgacg aaagaggtcc tgaccttcag ccagcacttc gcctgcccgg actgccatgt
     3301 cagcctgccg gaaatcgaat cccacctctt ttccttcaac agccccttcg gcgcctgccc
     3361 ggcctgctca ggcattggca gcaccatgga agtcgacgcg catctggtcc tgccggacca
     3421 caagaaatcc tttgccgacg gcgccgtagc ggccctcagc agcaaccccg attcgtggtt
     3481 catgcgccag ctcggtggcc tgctgcggcc ttacggcttt accttggata actgctacga
     3541 tgacttgctg gaagaattgc aggaaaaact catgaacggc tcagacgata cgtgcgtctt
     3601 tgaatacgag aacctgcgcg gcgaagtgaa gcagttcgcc acggcctttg aaggcatcct
     3661 gcccatggtc cgccgccgct accgcgaagc ctcgtcggac atgatgcgcg accagttcgg
     3721 ccagttcatg actgtcaagc cctgctcgac gtgccacggt gcacgcctgc gccacgaagc
     3781 gctggccatc aagatcggcg gcctgaacat cgccgaattg acggatttgc ccgtcagcga
     3841 catgattccc ttcttcgata acttgaaatt gacggaaaaa cagcagttca ttggcaagca
     3901 gatcttcaag gaaatcaagg cccgcctgaa cttcttgcag accgtcggcc tggactacct
     3961 gacattgtcg cggacggcgg gcaccctgtc cggcggcgaa gcccagcgta tccgcctggc
     4021 cacccagatc gggtcgggcc tggtcggcgt cctctatatc ctggacgaac catccatcgg
     4081 cctgcaccag cgggacaacg acaagctcct ggaagccctc aagcgcctgc gcgacctggg
     4141 caatacgctc attgtcgtcg aacacgatga agacaccatg cgtgctgccg attatatcgt
     4201 cgatatcgga ccggaagcag gggaacacgg cggcactatc gtcgcccaag gcacggccga
     4261 tgacatcatg gcctgccctc agtctttgac gggccagtat ctgaagggga ccaagtatat
     4321 ccccgttccg caagaacggc gccaggggaa tggcaccttt ttggaaatca tcggtgcagc
     4381 tgagcacaat ttaaaacata taaacgtctc catccccatc gggaccctga ccgtcgtcac
     4441 cggcgtgtcc ggctcgggca agtcgacgct ggtcaatgaa atcctctaca agggtatggc
     4501 cgaagccgtc tacggcacgc cgcaccggcc gggcaggttc aaggccctga aaggcgttga
     4561 atacatcgac aagatcatca acatcgacca gtcgcctatc ggccggacgc cgcggtccaa
     4621 tccggcgacg tatacgggcg tcttcgactc catccgccag ctctacagcc agacggcaga
     4681 agccaaggtc cggggctaca aggccggccg cttcagcttc aacatcaaag gcggccgctg
     4741 cgaagcctgc aaaggcgacg gcatcatccg catcgaaatg aacttcctgc cggacgtcta
     4801 cgtcccctgt gaagtctgcc acggcgcccg ctataaccgg gaaacgctgg aagtcaaata
     4861 caagggcaag aatatttccg acgtcctcaa catgaccgtc gacgacgcct gccagttctt
     4921 tgaaaacata ccgcgcatcg tcaataagct gaagacgctg cagcaagtcg gcctgggcta
     4981 tatccgcctg ggccagccgg cgacgaccct gtccggcggc gaagcccagc gggtcaagct
     5041 ggcgacggaa ttgtccaagc gcagtaccgg ccggaccctg tatatcctcg acgaaccgac
     5101 gacgggcctg catacggcgg atatccacaa gctcatggac gtcttgcaga tgctcgtcga
     5161 cggcggcgat acggtcgtcg tcatcgaaca caacctagac gtcatcaaga cggcagacta
     5221 cctcatcgac ctcggtcccg aaggcggtgc cggcggcggt acggtcgtcg ccatggggac
     5281 gcccgaagaa atctgccagg taccggaatc ctatacgggt cagttcctca agcccgtcct
     5341 ggaacggacc aaggccctca tggccggcaa gaagggggta taagccatgg ccgtcagcga
     5401 ggccgtccgg gaaaaagtcc gcaacctgcc gacccagccc ggcgtctatc tctggaagga
     5461 tgaaaagggc aagatcattt acgtcggcaa agccgtcaac ctgcgcaacc gcgtgaccag
     5521 ttacgtccgc cacgatgcca acagggcgcc gaaagtagcg gccatggtca gtcatgccgc
     5581 cgatttggaa accatcgtcg tcgccacgga aatggaagcc ctcatcctgg aaaatacgct
     5641 catcaagaaa taccatcccc attacaatat catgctccgc gacgacaaga cctatcctta
     5701 tatcaaggtc actgtccagg aagattatcc gcgcatcttc atgacccgcc gcgtcagccg
     5761 cgacggcgcc cgctatttcg gaccctttgc cgactcgacg gcagtccacc gtgtcctgaa
     5821 actcatgcag cgggcctatc atatccgctc gtgccggacc atgccgacag accggccgtg
     5881 cctgcaatat cacttgcacc actgtgatgc gccgtgcgtc cactatatta ccaaagccga
     5941 ctacggggcc cgggtccgac aggccctgaa tatcctcgaa ggccgggaca gcggcatttc
     6001 ccggtcgctg cagcagaaga tggaagacgc tgcggaagcc atggaattcg agaaggccgc
     6061 catgtaccgc gaccagatca aggccattgc agtcatccag gaacagcaga acatcgtcaa
     6121 tacggctggc ggcgatatgg acgtcatcgg cctggcccgc caggctggac agacgtgcgt
     6181 ccagatttat acggtccgca tgggcaagct catgggacgg gagacctttt ccctggacaa
     6241 cagcagcgat gacgacgtgc cgtctctgat ggaagccgtc gtcgaccagt attatagcga
     6301 cgggaccttc atcccgtcag aaatcgtcgt gccggccctg gaagacctgg acggatgcga
     6361 acgccgcctg agccagcaga agggcaggaa agtgaccgtc gtcattcccc agcgggggcc
     6421 gaagaaacag ctcctggaca tggcttccga aaatgcagcc ggcctgttgg aacagaaacg
     6481 cctgcaatgg cagcacgacg tagacaagac gaccggtgcc gtcgaaggcc tggcgcgcat
     6541 cctcaatctg ccctgcctgc cggagcgcat ggagtgcttc gatatttccc atacccaggg
     6601 gatagagacc gtggcctcga tggtcgtctt cgagcacggt cagccggcta agaaagagta
     6661 tcgccgcttc aaactgaaga ccgtccaggg caagcctgat gactttaagt ccatggcgga
     6721 aatcatggaa cggcgctatg gcgaaaagga ttggcccgtg ccggacctga tcatcatcga
     6781 cggcggcaaa ggccagctcc atgcggccct gccggtcatc cgccaggctg gctgcgaagc
     6841 gccggtcatc agtctggcca agcgcatcga agaagtcttc gtcgaaggcc gcagcgattc
     6901 catcatcctc agccatcaca cgccggaact ccagctgctg caggccatcc gcgacgaagc
     6961 ccaccgcttt gccattacct atcaccgtca cctgcggggc aagcgcagcc tcgtatccat
     7021 cctggaccac gtcgaaggca tcggggccaa gcggcgcaag gccttgtgga cggctttcaa
     7081 gacgctggcc gacatgaagg ccgcttccat cgacgaactg gctgccgtgc cgggcatgaa
     7141 ccgccaggct gcggaaaatg tctattattt ctttcgcctg gggacggatg aaaagaggaa
     7201 attgacccaa tagatatgat ataatagaaa gaaatgaaag aggagggatt atgatgatga
     7261 aaaagaaagt cctcaccgtt ttatgcggcg cctggctcat gggggccgcc gccatggccg
     7321 cttcgccggc cgtcatccag gatgcgccca aagccgtgta tacccatagc gatggcagcg
     7381 tcctcagtat ccaatacccg tccatcacga tgacggaaaa tgccggagct gcccagctga
     7441 tagcccagta ttttgccgat gaacagcagc aggccaagac ctttttcgac cagcagggca
     7501 ataaaggcgt caaattgacg gaagagaaga cctacaccgt caccttgaac gacggcaaat
     7561 atttgtcctt cctcgatgaa ggctatctct atttcgacag ggctgcccat ccgaccagct
     7621 ggaagaccgg cgtcgtcttc gatatggtga cgggcaagcg catcaccaac tggcgtgacc
     7681 tggttaaacc gggcgatgaa aagtatttca ccctcaagaa aatcaccaat aaactgaccc
     7741 tgag
//
LOCUS       DAWABZ010000085         7664 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2578_length_7664_cov_3.178210, whole genome shotgun sequence.
ACCESSION   DAWABZ010000085 DAWABZ010000000
VERSION     DAWABZ010000085.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7664)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7664)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7664
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2578_length_7664_cov_3.178210"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1548 a         1911 c         2174 g         2031 t
ORIGIN      
        1 ggcgctttcg atggcgacgt cggtcccggc accgatggcc atgcccagat cggccttggc
       61 caacgccggc gcatcgttga tgccgtcgcc gatcatggcg actttatggc cttcggcctg
      121 gagggcggcg atgtgttttt ctttatcctg cggcaggacg ccggcgatga cctggggaat
      181 atgcaggcgc tggcggatgg cttcggctgt gcgctggttg tcgcctgtga gcatgacgac
      241 gttgatgccc attttggcaa agtcggcaat ggcttcggcg ctggtcgctt tttcgacgtc
      301 ggctacggcg atgataccga tgatttcttt ggcatcggca aagaggagcg gcgtcttgcc
      361 gtcgtcagcc agggcattga ggctggccgt gatggctttg gtgtcgatat gggcttcgtc
      421 catgaggcgc gtgttgccgg cataataggt cttgccgtcg atgacggcct ggacgccgcg
      481 gccgaagagg gccttgaaat cgctggctgt cctgccggtc agctgatggt cgccggcata
      541 ggtcgtaata gcttcggcca ggggatgttc gctgcctttt tccagggctg cagcgatggc
      601 gacgaggtct tctttcgtgc ctttcagggg aagaatatcc gtgactttcg gacggccttc
      661 ggtaatggta ccggtcttgt ccatgacgac cgtgtcgatg gcatgggccg tttccagggc
      721 ttcgccggat ttgatgagga taccgttttc agcccctttg ccagtgccga ccatgatggc
      781 gactggcgta gccaggccca gggcgcacgg gcaggagatg accaggacgg agatggccgt
      841 cgagaaggcg aattcggcgt cgacgcccat gacgaagtac cagatgaggc ctgtaatgat
      901 ggcgatggtg atgacggccg ggacgaagta acccgaaatg gtatcggcca tcttggcgat
      961 aggtgccttg ctggcactgg cttcatcgac gagcttgatg atctggctga tcgtcgtgtc
     1021 gtcgccgacg cggcgggctg tgatgtggat gaaacccgtc ttgtttatcg tagccgacgt
     1081 gactttatcg ccgacttgtt tttctacagg gatgctttcg ccagtaatgg cggcttcatc
     1141 gacgctggtc gtcccttcag aaatgatacc gtctgccggg atgctttcac ccggacggat
     1201 gacgatttca tcgccgacga cgaggtcttc gacgggaatg accgtttcga cgccgttgcg
     1261 caggaccgtg gcctgtttcg gcgccaggtc catcaatttt tcgatggccg tgctggtctt
     1321 gcctttggcg cgggcttcga gatatttacc gacgtcgatg agggtgacga tcataccggc
     1381 tgattcaaag tacagattgg tgctgtactg ggcgacgagg gcccagtcgc catggcccat
     1441 accccagctc atgcggaaaa tggcgaagac gccgtacagg gcagctgcca tggaacccat
     1501 gccgaccagg ctgtccatgt tcggggcgcc ctggaagagg ttgcggaagc cgttgatata
     1561 atacttgcgg ttcaagtaca tgatcggcag gatcagtaag aactgagtaa aggcaaaggt
     1621 aatggcattt tcatcgccgt ggaagacgta ctggaaacct tccggtacgg ggatgccgaa
     1681 ccattcgtag aacatgtggt gcatggcgat gtacatgacc gggatgagga agacgatgga
     1741 ccagatgagg cggtgcttca tggatttcat ttcttccttg gctatgtcag aggcgctctt
     1801 ttgtggagcg ccggcctgtt tctgactgcc gccttcggcc aggctggctc cgtagccggc
     1861 gtcgacgacg gcctggatga tgtcttgctc gctgacgaca ttttcatcgt acgaagcctg
     1921 catactgttg gtcagcaggt tgacgctggc cttgtcgatg ccgtccagct tgttgacggt
     1981 tttttccacg cgggacgagc aggcggaaca gctcatgccc gtgatgttga atttttcgtg
     2041 cttcaacgga ctcactcctt tatttcatga gtttctgaag ggcgcagacg agttcatcga
     2101 cgacttcgtc tttgccttca cggatgtcgt ggacgacgca ggacttgata tgctggccca
     2161 ggagttcctt attgaaagaa ttcaatgccg actggatggc actgacctga gtcaggatat
     2221 caatgcagta gcggtcgtct tcgaccatct ttcgcacacc ccgtacctgg ccctcgatgc
     2281 ggttcaggcg ggaaatgagc gatttatact ctttgccctc tttgttgcga tacttatgtt
     2341 tctccgtatc gcagcaggga cagactttct tttcgtccat aattgagttc tcctttcatt
     2401 taaatacccc tatggggtac attgatataa tataccgata tggggtatat gtcaaggggg
     2461 ttttagcggt tagtagttag gggctagcgg atggttttta atttgtaggg gcgaccgcgt
     2521 gaggcgcccg ctccggaata tgcatgattt cctaatcagt ttgccaggag aatcatccca
     2581 attcctaacg ggtcgcccac ggacccctac gaacgctaac cgcggattgt taaaaaaagg
     2641 actacgtatg atgatataag ctctcatacg atagtccttt ttctgtaggg gcgtccgccc
     2701 cgggattatg cgatttcact gtaggggcga ccgcgtgggg cgcccgctcc ggaatatgtc
     2761 tgatatccca atcagtttgc caggggaatc atcccagttc ctaacgggtc gcccatgagg
     2821 ccgccacgag ccactaacac taaccactaa ccactaacca ttaaaaaaag ctgccgcaaa
     2881 gcgacagctt ttttactcct atttcaacaa tgcttttaca gcttctgttt catccgtagc
     2941 gttttcagta ccggagatga tggcaccctg gactacgatg cagtcgaggc ctttgtcctt
     3001 gcggacctgt tcgactttgg aaacgacgtc tttttcaatg gggacgatca gtttgttttc
     3061 caatttgctc aagttctgag ccaattcacg gccgaggttc tggcgttcct ggtcggtctt
     3121 cattttcgga gcttttttag cgaactgttc gttagcgtct ttaacggcag cctgcatctg
     3181 ggcgctgact ttcgggaagt ccttatggga ctgtaccaat gtatcgaagt tgacgacacc
     3241 gacgccggaa gcaaaggccg acgaaacagc gacgaggctc atcattccca taacggcaag
     3301 ggatgctaat tgttttttca gtttcataat gtattcctcc tgtgccgcag agcggctgaa
     3361 ttactctttc ttactatggt tctattcgta tattataaca aacttaccga taaagtacaa
     3421 cctgcacaga atcaggcttt ttcgtcgtcg ccttctgctt tgtcttcttc atcgtcgtga
     3481 atataaatgt atttgtcttt tttcatcttg tcgatgattt ccggtgccat ggcgatgagt
     3541 ttttcaaagg ccgaagcctg gtagccatct ttcttgatgg agaacatttc tacccggtcc
     3601 cctttgatga ccaggatggc agctggttcc atcttggcac cgccgccgct gccggcactg
     3661 ctgcgcccgt tttcggtctt gccgttgacg ccgccggacc cgaagccaaa ggtgacgtct
     3721 acgaacggta ccaggatggc gtcaccgatg tggacggcct ggccgacgac ggtttcgact
     3781 ttgatcattt ctttgaaact ttcaaaaatt tgttccaagt tgttcttgat gtacgagtta
     3841 tccatgttgt tcctccttgt tcacagcgtg ccactcagcc cataaacggc gtacaggccg
     3901 ggaggcggca aaggcggctg taaacagaag cagcactgcc ggatatagac ggcccgaagc
     3961 acggacggta cagtcatact gttcctctac gaaattgaat tgtaagtctg tgacgctccc
     4021 cggacagatg gcatagaggg caccggccag catccccgtt tcatgcggtt cagggaggcc
     4081 gacgatgccg gtcagggtga agcggcggat ttggccgtgg ccgagcaggc gcgacagcca
     4141 ggtgaagaaa gccgctaaaa agtcggcatt gaagaagctg gctaagaacc ggcgtatggg
     4201 cggtttcttt tttttcgctt ctttcgcgtc actctgtgct ggggcatccg gcccttcctg
     4261 gcggagcagg tcttcatacg tgaggcgctg gctttctttt tccagttcgt cccaggcctt
     4321 ttcttcctct tccgctgtag ggagcggcgg ttcgacagac tctttcacgg caaggctgcg
     4381 cttccagcac atatgcagtt catgatgcgg ctcctcgccc agcgtatagg accaggatgc
     4441 atcggccaat ttccggcccc atcggccctg gccttcgatg tggaaaggcg tcccgatatg
     4501 gatggtaagg gcgtaggaga cgggcaggca gaggatgagc agtatgcagc tgaataagat
     4561 ggcaaggatg acgatgattg ccatagggca gccccctttt gtgaaaataa tccgttactt
     4621 ttatttattc gatgaactcg gggcgatttc ctctttttta ggcgtcttgt ttcgcttttt
     4681 cttgtttatt gaaaatgcag tccacatctt cggcaccgaa gcagcgggaa caggaaatgc
     4741 acagggccgg ttcggtatcg cccgatttcc agcggttcgg caggtccggc tggcgcaggt
     4801 agggacggct catggacacg aaagccaggt ctgtcgattc cagtgtatct tcgatatctt
     4861 cgaccttgcg gaaaccgccg acgacgccga catcgatttt gtctttcagc atagctgcgg
     4921 cttcagaggc ataggctttg aaatacatcg gttcctttgt gcggcggata gcccggatcg
     4981 ggcccttgcg cggcagggac gatgtgttgc cgccgctgac ttcgatggcg tttacaccca
     5041 tgtcggccag ccaggtcatg acctgcttgc tttcttcaaa ggtcatgctg ccgtctttca
     5101 tgaaatcggc acagttcaat ttgacccaga ccggataatc ggcgccgacg gcctggcgca
     5161 tggcggcgac acattccagg aggaagcggg cccggttttc caggctgccg ccgtaggcat
     5221 cggtgcgggt gttgaaatgg ggcgacaaga acttgctggc caggtagccg tgggcgccgt
     5281 ggaactggac gccgtcgaaa ccggcggcct tggctctggc agcggctttg gcgacggctt
     5341 ccgtcagttc gtggatctgg tcgactgtca gggcctgcga ttcctgggtc cacttgccga
     5401 ccttttccgt aacgccgctg gggctgatga taatgcgcgt ttccccttcc gggatttccg
     5461 gcaggaggat ggcggacccc atgacgacga gctgggcgat gatttttccg ccgtgctggt
     5521 ggacgcggtg gacgacctgg gacagcgggg caatacattc atcatcgtcg atgcggatct
     5581 ggcagtgctg acgcggttcc attttggtag tagccatcat gccggtaata atgataccgg
     5641 caccgccgtc ggccagattt tcatacattt gggccagctt ttctgtcgga aggccgtagt
     5701 tccccattcc ttcaaaggta gcagaacgga caaggcggca cttcgcttca ataccgccga
     5761 tgtgacatgt atcaaataac atggtagaaa tctctccttg gtaataaaaa atgaatttca
     5821 gtttatcccg aatattatac aatatttgag gaaataagcc aatgcattat tttttgaaca
     5881 tttgggagtt cattttaaaa agggcttgtc gctattcact tattgggatc atcaggagat
     5941 tttttacctg tttctcgttc atattcatcg aaatcaattt tgctttttga accgagttcc
     6001 aatgcttttt tatagtacca ttctttaaaa tccagctctt ccatgtagta ctgcagttgt
     6061 tcaatctgat ccaggacaaa ctgacgctgc tggcggatga gggctgacgc tgctggcgga
     6121 tgagggcctg ccgttctttc aggctttcat cgccgcgctg agccagttcg gcatattcgc
     6181 ggatgcgctt gatgggcatg cccgtatttt tcaggcaggt cagcaggtgg agccagcgaa
     6241 tatccatatc gctgaagaga cggataccgt ttttccgttc tacctggggc agcagccctt
     6301 ctttgtcata atagcggatg gcagacggac tgataccagt catctgcgcc gcttgtgaaa
     6361 tcgtataaga catagtttat ctcccttatt ttaaatactg ccggtagaag gcttcgattt
     6421 tgtcgaaggg aatgacatcc attttataat acaggtcggt gtgtacggca tcggggatga
     6481 tcagcagttc tttgttgttc cctttcagtt tcttgaacgc gtcttcgctg aagtagcggg
     6541 agtgggcttt ttccccgtgg atcatcagta cggcgctttc gatttcatca gcccgggaca
     6601 gaaggggcat gttcatgaaa tcgagcaggg aactgagagt tgcgcctgtg gtggagccga
     6661 aggaccgggg gctgtagccg cgtttttcct ggtagtagtc atgataatcc ttgacgaact
     6721 gtggcgtatc ggctgttact tctgtgggga caccgccggc cattttatag gtgccattcc
     6781 ggtaatcttc tgtgcgctgt gcgctgatgg ctttgcgggc ttccatccgt tcttttttta
     6841 gggttgcttc atctttttca tagtcgaaat agccattggc gatgacccgg ctcatgtcgt
     6901 acatggtaga tatgaccgta gctttgacgc gcgtatccat ggcagcatca ttcagagcaa
     6961 agccgcccca gccgcagatg ccgaggatgc cgattttttc cggggcgatg ttattgcggg
     7021 tggacaggta atcgacggcg gcagaaaaat cttccgtgtt gatatccggt gaggtcgtgt
     7081 tgcgcggttc gccggcgctt tccccggtaa aggacgggtc gaaagccagg gtgatgaatc
     7141 cccgggccgc taattcctgg gcatagcggc cggaaacctg ttccttaacg gcaccataag
     7201 gaccggctac ggcaatggca gcgagttttt caccttgttt catatttttc ggcgtataga
     7261 ggtcgccgac taacgtaatg ccatagcgat tatggaaggt aattttttgg tgagcgacct
     7321 tcgcattttc tggaaacgtc ttgtcccatt tattggaaag tgttgcagcg tcaatggttt
     7381 cagccggtgc tttcgatgga gctggggcct gggcggcata actgctaccg gcacctgcgc
     7441 cgatgatgaa aagggagctg cacagggcag cgaataccat atttttcatg atgcgttttc
     7501 ttaatttcat aattataggc ctcctgtcag aaaatttata gctcatttgt atttcatggc
     7561 ctaagcataa accttaaagg taactttaag tcaagggttt tctttagcgt gatttcttta
     7621 taacccccaa aaccttttga gttcattgac aaaatgggct taaa
//
LOCUS       DAWABZ010000086         7658 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2585_length_7658_cov_3.375510, whole genome shotgun sequence.
ACCESSION   DAWABZ010000086 DAWABZ010000000
VERSION     DAWABZ010000086.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7658)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7658)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7658
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2585_length_7658_cov_3.375510"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1894 a         2320 c         1997 g         1447 t
ORIGIN      
        1 acaactgggc cgtcctgcga ttcaggatat cgtgtactca tattgttaac cttgatacaa
       61 acgaaataaa gttgactgca atcgccgcag cgagtataat aatcccatag ttcacaataa
      121 aaatagggag tgggttgaca atgaatacga tagcagcact gaccgatagc attatacagg
      181 ggtaccgcat ccaacgcggc gatgatttat cgattttcct ggaagcacct ttggaagaat
      241 tgcagcaggg agccggcgtt ttacagcgcc atttttgtgg caatcatatt gatttctgca
      301 ccatcatcaa cggccgcagc ggccgctgtg gggaagactg caaatactgt gcccagtcgg
      361 cctgccacca tacgggctgc gaagaatacg gtttcctggc gaaagaagaa atcctggcca
      421 acgccaaggc caatcaggat gccggcgcca accgctttgc catcgtcacg tcgggccggg
      481 ctctgacagg ggcggatttt gaaaaagccc tggatacgta ccgcacgatg aagcagacgc
      541 tgcacatcga cctctgtgca tcgcacggcc tgctgacgcc ggaacagctg caccgcctcc
      601 acgaaacggg cgtgaccagt tatcaccata atatcgagac gtcgcggcgt ttcttcccat
      661 acatctgcac gacccacacc tatgacgacc gcatccgcac catcaaggcc gctcagaaag
      721 aagggctctg cgtctgttcc ggcggcatca tcggcatggg ggaaacctgg gaagaccgcc
      781 tggacatggc catcagcttg gccgaactgg gcattcagtc cattcccgtc aacgccctca
      841 tgcccattcc cggcacgccc ctggaacatc ggccccagct cgacggccgc gatgtcctgc
      901 ggaccatcgc cttcttccgc ttcatcaatc ctgaagctaa catccgcctg gctgccggcc
      961 gcaaactcct gccggaaaac ggcgccacgg ccctgctcca cggcgcgtcg gcctcgatta
     1021 cgggcaacat gctgaccacg tcgggctcga ccatcgccga agacatggcc atgctccagc
     1081 gcctgggctt gactaattcg taggtcgtag gccgtagatc gttcgtcgtt gatggtacga
     1141 ttaaaaatca taactaatac gacgaacgat gaacgaaaaa cgtttgaatc tgcgagggct
     1201 atgtgcatga tagtctatac tggtgtatga caagccctgc actagccttc atcctttgtc
     1261 tatgatataa tgttagggat atagaaacat acatatactt ttcaatgagg tgattatcgt
     1321 ggcaaaacag atttttgtta ccggtcataa gagcccggac acggactcca tttgttcttc
     1381 cattgcctat gcaaaattaa aacagttaca gggtatcgac gctacgccga tccgcgctgg
     1441 cgaaccgaat aaggaaacgg cctttgccct gaattatttc cacgttgctc cgcagccact
     1501 ggtcaaggac ttctacgtca aagtcagcga agctgtccac acggaagtcg aagcccttcc
     1561 ggtcagtgcc agcctgaaag atgctgccaa atggttcgca tcccacgaaa gcgatgtcgc
     1621 tcccgttgcc gataacggcc agttcgtcgg cgtcatcgaa aaaggccagc tcgccgatga
     1681 attcgtcaag accgtcatgg gtgaagaatt gagcacggtc aaagccttgg ctcacgatcc
     1741 ggaattgttc ttcaagccga ccgataacgg ccatgatatc ccggctgatg gtaaatacgc
     1801 tgtcgtcgaa aacggcaaat acgtcggcat gatctgtgca gactgtgtcg ctgctgtcga
     1861 aaaacagcag gtcatcctcg tcgaccacaa cgaaaagaaa cagatcatcg acggcatcga
     1921 cgaagcatct atcgtcggcg tcatcgacca tcaccgcatc ggcggcactt tggaaacagc
     1981 cggccctatt tccatcctct tcaaacccgt cggctgcacg gcaaccatcg taacgggctt
     2041 gtatgaacag gctaaggtcg acatccccaa agacattgcc ggcctgctct tgtcggccgt
     2101 catttccgat acggtcctct tccgttcgcc gacctgcacg ccggctgacc gcgaagctgc
     2161 cgaaaaattg gccaacatcg ctggcgtaga cgtcgaaaaa tacggcatgg aaatgctcaa
     2221 agccggtgcc aacgtctccg atttgacgcc ggaccagatc gtccacaacg acatgaaaga
     2281 attctccagc ggcaatacga ccttcaccgt tgcccaggtc caggtcatgg atacgacgga
     2341 cctcctcgac cagaaacagg tcctgctcaa cgccttggaa aaattgcgcg ctgaacacga
     2401 ttacgccgct tccttcctga tgatcaccag catcatcgac gaatcgacga acctcatctt
     2461 cgccggtaat atggacgacg tcgtcagcag tgccttcggc aaggacgtcc tcgataagga
     2521 agtctacctg cccaaggtca tgagccgtaa gaaacaggtc attccgccta tcctttcggc
     2581 aatgaaataa gtgatgattt tcagaggccc catcgacacg gtgtgggcct ttttatctat
     2641 aaaggggaag aatgcatgaa tcctattttt gataccctca acgaccgcca gtgcgaggcc
     2701 gtcaagcata ctgaagggcc gctgctgatc acggccggtg ccggatcggg caagaccaaa
     2761 gtcctgacct gccgcattgc ccatctcctg gaattaggcg tcgcgccgta ccgcatcctg
     2821 gccattacct ttaccaacaa ggccgccaag gaaatgaagg aacgtgtgac caacctcgtc
     2881 ggtgcccagg ccgacagcat ctggctgagt acgtttcact cgttctgcgc caagctgctg
     2941 cgctttgaaa tcgacggctt ccatggctat acgcgcaatt tcaccatcta cgattcgtcg
     3001 gaccagttgg tcctggtcaa ggactgcctg aaaaaactca acctcgacga caagcagttc
     3061 acgccgcgct ccgtgttggg gaccatttca tcggctaaga acgtcctcat ggatgccaag
     3121 gcctttgcgg cgaaagcctc cgatttctac gaacagaaag tcgccgacgt ctacgctctg
     3181 tatcaggaaa aattgcggga aaacaatgcc gtcgatttcg acgacctttt gttcctggcc
     3241 gtgcgcctct tgcaggaaaa cgaggaagtc cgggaaaaat accagtcccg gttccaatat
     3301 atcctggtcg acgaatatca ggatacgaac catgcccagt atgccctgac caagatactg
     3361 gccgcccgct ggcgcaatat ctgcgtcgtc ggcgatgccg accagagtat ttacgcctgg
     3421 cgcggtgccg atatccgcaa catcatcgac ttcacgcggg actatcccga tgcggcgtcg
     3481 atcaaactgg aacagaacta ccgctcgacg aagactatcc tccatgcggc caatgccgtc
     3541 atcgacaata acgaaagccg gcccaagaag accctgtgga cagagaaccc gacggggaac
     3601 aagatcatcc attaccaggc ccagacggaa cacgacgaag ccgattatat cgccggcgtc
     3661 atctacaacc gccatgaaat cagccatgaa ccgtatggcg acatggccat cctcttccgg
     3721 accaacgccc agtcccgtgt cctcgaagaa aagctcatgc gctatgccat tccctatacc
     3781 atggtcggcg gcaccaagtt ctatgaccgc aaggaaatca aagacgttct ggcctacctg
     3841 cgcctgctct ataacccgga agacagcctg agcctgacgc gcatcatcaa cgtgcccaag
     3901 cgcaacatcg gtgcgacgac gatggaacac gtcgcggcct atgccgaagc acaggggatt
     3961 tccttatttg aagccctgtc gtcgaccgat gagatccccg tcaccaaacg gggcaaggca
     4021 tcgctggaaa acttcgctgc catgattttc gacctcctca acgacatcga aggcaaagac
     4081 gtcctgagcc tcattgaaac ggtcatcaaa cagaccggtt acggcgacat gctcgataaa
     4141 gaagcggaac acgacccgca gggcgaaagc cgcaaggaaa acgtcggcga attcctgtcc
     4201 gtcgccaaag actacatgga cagcaatccc gaaggcaacc tccaggactt cctggaaaac
     4261 gtcgccctcg tcagcgatgt cgatgatttt gaaagcagcg actcgaaagt cacactcatg
     4321 accctccatg ccgccaaggg cctggaattc ccggtcgtct tcctgaccgg cctcgacgaa
     4381 ggcctgttcc cgcacagccg gaccctgatg gacccagcgc aggtcgaaga agaacggcgt
     4441 ctggcctatg tcggcattac ccgggccgaa cggcagctct acgtgaccaa tgccgtcacg
     4501 cggaccatgt acggccgcat ctcggcctat atgcccagcc gtttcctggc cgaaatcccg
     4561 ccgcagttca tggaagacta ccatcgcaag agcgccatgc cccagagccg gacgacggcg
     4621 gtcccgggga aacagcgcgt ctccatcctg accaagcccg tcgcgtcgag cctgcccaag
     4681 aaacatgccg ttaccgatac ctttgccaaa ggcgataaag tccgccataa aatctggggc
     4741 atcggcaccg tcctcgacgt catcggcgaa gggccgaaca tgcagatgaa aatccaattc
     4801 ccgaccaaag gcgtccgcca agtcgtcgtg aaatacgccc ccttagagaa aatacagtag
     4861 gggagcctaa gccttccata ttaggccctt tttggcccgt caggtgctga ccacaggaaa
     4921 gcgcaggacg gttgccaaaa cggagagctg agctggaggt ggcccaacgg gccggagagg
     4981 ttgcttgcac ttttgcaggc cgcttcggag agctctcggc tcgtaaaaat gccccggccg
     5041 ggctttcgta ggggtcgcca cgtgggcgac ccgccacaga acttgcgatg agattctttg
     5101 caggacgaat ggaaacgatt ttcatgaatg aaggagaaac ccatggatac tgaaaaagcg
     5161 cggctcttgg agctgcggaa aaaaatagat gaattgagtt atcaatatta caccctcgac
     5221 aagccgacag tgactgatta tgaatacgat atgatgtacc gggaactgga aaacatcgaa
     5281 aaagcccacc ccgactgggt cacgcccgat tcgccgacgc agcgcgtagg ctccaagatt
     5341 tccggcggct ttgaaaaata tacccatgac cggcccatgc tcagcctggg cgacgtcttc
     5401 agcgacgatg aattgcggga attcgaccag cgcgtccgcg atgacctggg cggcgaagat
     5461 ttggaatacg tcgtcgaact gaaaatcgac ggcctggcag tcaatctcat ttatgaacag
     5521 ggtcagttca tccgcggcgt cacccgcggc gacggcgtcg tcggtgaaga cattaccaac
     5581 aacgtccgga ccatccgcac cattcccctg cacatcgcca gcgattcgcc gcacatcgag
     5641 atccgcggcg aagtctacat gcccatcacg tcgtttgaag ccttgaacca gcagcgccgc
     5701 gatgacgaac tggaaccctt tgccaatccc cgtaacgccg cagccggctc actgcgccag
     5761 ctcgacccgc gcattacggc ccagcgcaaa ctggctttct ttgcctacgc cttgggcgga
     5821 aacagcggca tcaccatcga gagccaggaa gaactgctcc aggacttggc ccagttccat
     5881 ttccaggtca atccggaata ccgcaagttc acgaatatcg aagacgtcat cgccttcatc
     5941 cacagttggg acgaccgccg cgacagcctg ccttatgcga cggacggcat ggtcgtcaaa
     6001 gtcaactcct ttgcccagca ggcccgcttg ggcaataccg tcaagattcc ccgctgggcc
     6061 attgcctata aattcccgcc cgaacaggcg cggacgaaag tcctggacat ttccgtcagc
     6121 ctcggccgga caggcgtcct gacgccggca gccgacctcg aaccggtcca cctggccggg
     6181 acgacagtca aacgggcgac gctgcacaat gaggattaca tcaaagaaaa agacatccgc
     6241 ataggcgata ccgtcgtcat ccagaaggcc ggcgaaatca tccccgaagt cgtccgctcc
     6301 ctgccggaac tgcgcgacgg ctcggaaaaa gtcttcgcca tgccgacgac ttgtcctgcc
     6361 tgcggcagcc ctgtcgtccg ccgcgatggg gaagcggcct ggcgctgtac caatccggac
     6421 tgcccggctg tcatcggtga aaaaatcgcc catttcgtct cccgcgatgc catgaacatc
     6481 gacggcctgg gcgacgccat cgtccagcaa ctcctggccc agcatctgat ccacgacgta
     6541 gccgacatct acggcctgac taaagaagaa ctggtcgacc tcgaaggctt tggcgacaaa
     6601 tcggcagaca acctgctcaa agccatcgaa acgagcaaat ccgtcggcct ggcccgggtc
     6661 ctctttgccc tgggcatccg cttcgtcggc gccaaagccg gccgcgtcct ggccgaatcc
     6721 ttcggctccg tcgatgccct catgaaagcc aatgccgatg acctgacagc tatcgacgac
     6781 atcggaccgc gcattgccga cagcatcgta gcctacttca gcgacgccaa gaaccaggcc
     6841 ctcatcgaaa aactgcgcgc tcacggcgtc gatatgacgg cagaaaagaa aacactcatc
     6901 aatacgacct ttgacggcga aatcgtcgtc ctcaccggca aattgcagat gttcagccgc
     6961 aaagaagccg aacaggccgt cgaagcacga ggtggtaaat gcacgtcatc ggtcaccaaa
     7021 aagacgaccc ttgttgtcgt cggagccgac ccgggcagca aatacgaaaa agccaagaaa
     7081 ctggggaccc ccatcatcag cgaagccgaa ttcaaagaat ggctcgaacg ctaaataagc
     7141 cacctctcag tcagcagagc tgccagctcc cctacaaagg gagccttaag cctcccctca
     7201 taggcccttt ttggcccgtc aggtgctgac cgtagggaag cgcagggcgg ttgccaaaac
     7261 ggagagccga gctggaggtg gcccaacggg ccggagaggt tgcttgtact ttcgcaggcc
     7321 gccggtcgca gttcgcagtt cgcaggccgc ctcggactca gatgccgctg tgcggcgaca
     7381 gactcagact aacaacgacg cttcgggaaa ccctccgcgc gtagcgggcc gccctctggg
     7441 acggccccta cggatgccgc ggcgcggcga cagtagaaat gcccccggct ggggcgtagg
     7501 ggtcgccgcg tgggcgaccc gccacagaac gaatgggaat acctaaacag actgaatgga
     7561 gatgatttta tgcgtgctat ggataaagta atgaaagaat tacatgcatt tattgctaag
     7621 aaggctccga acggtgttaa gagcgaagaa gagatgca
//
LOCUS       DAWABZ010000087         7629 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2600_length_7629_cov_2.949036, whole genome shotgun sequence.
ACCESSION   DAWABZ010000087 DAWABZ010000000
VERSION     DAWABZ010000087.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7629)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7629)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7629
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2600_length_7629_cov_2.949036"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    263..273
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1358..1367
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1933 a         2078 c         2013 g         1584 t
ORIGIN      
        1 tatttatagt aagttaatat cacttatagt gagtaagaat gtatgtaagg agatgaatgt
       61 catgaattta tctaatactg ccaaagtaat caaggaatct gagacaagaa aacttcttac
      121 cagcaaagga aatcaagaaa taaaagaaaa aatccgtaag gcctctcatc tcatgtttca
      181 acgcaatgaa aaagcctata gggacttaga aaatcgatga aacacgagca aatattgata
      241 gaaattatcc tagaagatgt acnnnnnnnn nnntgcgttt ttgatttggc ggagcgggtg
      301 ggattcgaac ccacgagacc tttcggccta actgatttcg agtcagcccc gttatgacca
      361 cttcgatacc gctccataag gtactttact agtatagcat ggtatgcgct atccgtcaag
      421 tttttttgca tatttatttt tgacagttgg tttacaaaga aaatgttgac ttagagtgta
      481 cttcaggtgg tacaatgaaa gtatcttatt gttagggggt aaaaatatgg aaaaagccaa
      541 ggtatatttc acggacttcc gctgccgtcc ggggaaaaat aatttacaga aactgcagaa
      601 gctctgcaag aaggctggta ttgaaaatat caattttgaa ggaaaattcg tcgccatcaa
      661 gatgcatttt ggggaattag gctgtctggc ttcgctgcgc ccgcagtacg tcaaagccgt
      721 ggccgatttg gtcaaggaat taggcggccg gccgttcctg acggactgca atacgctcta
      781 tccgggcagt cgtaagcacg ctttggaaca catggattgt gccaacctca acggttataa
      841 tacggtcact acgggctgtc agatcatcat cggcgacggc ctgcgcggca ccgatgaaat
      901 cgaagtgccg gtcaagaacg gcgaatacgt cgaaaaagcg aaaatcggcc gggccatcat
      961 ggatgccgac gttttcatca gcctggctca cttcaagggc catgaaatga cgggctttgg
     1021 cggcgccatc aagaatattg gcatgggttg cggcagccgc gccggcaaga tggaacagca
     1081 ttcgtcgggc aaaccgaaag ttgacgaaag caagtgcaag aactgtcatc gctgcgctca
     1141 ggaatgcggt tctgacgcca tcacttatga agataacgtc gcccacatcc acgaagactt
     1201 gtgcaaaggc tgcggccgct gcatcggcgc ctgtgctttt gatgccattc agactgtcga
     1261 atgggatgcc aatgaaaaac tggaccgcaa gatggccgaa tacgcccagg ccgtctgcca
     1321 ggaccgcccg tgcttccaca tcaacctggt catggacnnn nnnnnnnaca tttcgccgaa
     1381 ctgcgactgc catgccgaaa acgatgcacc tatcctgccg gacatcggca tgttcgcttc
     1441 cttcgacccg gtcgctctcg accaggcttg tgccgatgcc tgcatgaaag ccgctcctat
     1501 gcccaacagc cagttgtcgg ataacctggc taaacccgat tggcagcacc atcacgatca
     1561 cttcctcgac agcaatccta atgtcgactg gaagacgacc ctggaacacg ctgaaaaaat
     1621 cggcttaggc acacgggaat acgaactcgt ccgcgtccgc tgattaaagc taactactaa
     1681 agtttgagtt aaaatctatg attactatta tagcaagtat attgctatag tgtaattcca
     1741 taaaggataa atgtatattc acgattcgcc ttttagacag tggagtctat aaatacgcag
     1801 gaactaaacc ggaaggggcg ccggtttggt tcctgcgttt ttttatgtga agatatactt
     1861 cttaagaata cgttaccaag ctgaaacagg ccggtatcct gacgggttat cccgatggga
     1921 acttcgacgg cgaccgcatg atgacgcgtt atgaatttgc tgccatcgtg taccgggcca
     1981 tcatggccgg tgccgcttcc aacccggctt tgagccagga cggcacgctg gataaattag
     2041 cgaaagaatt ctcgccggaa atgaaacaca tccgcatcga tacgattgcc aaagataaga
     2101 atggcaaacc gacgatcgaa cgagtccgcg tcattcccga agcaaaacag tagaggaggg
     2161 gagtatcatc gcacagatag gattgtcttt tcgtatcgat tcagacctca aaaaggagtt
     2221 tgaagcattt tgcgatgctg ccggcatcag catgacggca gccatccatt tgttcatcaa
     2281 gacaaccgtc cgggaacaac ggattccctt tgaaataaag gcttcgagca aaaaatagaa
     2341 tcgagagagt aaagagaaag gacttgtcgc acggcgacaa gtcctttcaa gctgttagca
     2401 gctaggggtc aggggttagc agatatcgtt cgtcataaat cccggctggg cacaggattc
     2461 acggcggcct gcgaactgcg accggcggcc tgcgggccac gagcccctaa aaagcccttc
     2521 acaggggcgg cctatatatg gtattatata ctgtaaaccc taaattatga tttttgtgaa
     2581 aaacggagag aatacatgga ttttcagagc aatcgatttt atcatattat ttcttatggc
     2641 tgtcaggcga atatgtctga cagtgagcat tatgccggtc agttggagga gctgggctat
     2701 cacaatacgg aagatttgga tacagccgat gtcatcctca tcaatacgtg ttgtgtccgc
     2761 gaaacggcgg aagacaagac gctggggaaa atcggcgaac tcaagcattt gaagaagcag
     2821 aaccccgatt tgatcattgc cgtcacgggc tgcatggccc aggaatggca ggataagctc
     2881 ttcaagcggg cgccgcacat cgatttggtc atcggtacgc acaatatcca caagctcatc
     2941 gagctcatcc gggaacggcg cagcaaggcc gaccattata tggctgccga catgaccctg
     3001 ccggctttcc atgatatgcc ggtcaagcgc ttccagaaat tctttgcctg gatccccatc
     3061 atgaacggct gcaacaagtt ctgcacgtac tgtatcgtgc cttatgtccg gggccgtgaa
     3121 gtgagccgtc ccatcgacgc cattgtcgaa gaaatcaaga aggtcgctgc tgaaggctac
     3181 aaggaaatca cccttctcgg ccagaatgtc aattcctatg gcctggacct caaggacggc
     3241 acggatttct ccaagctcgt cgccgctgtc gacgatatcg acggcatcga acgggtccgg
     3301 tacatgacca gccaccccaa ggatatgact tttgccatga tcgacgccat tgccgacagc
     3361 aaaaaggtcg tcaaccacat gcatctgccc atccagtgcg gttctgacga gctcttgaag
     3421 aagatgaacc gcggctacac ggtggaacat tacctggaac tcgtcgacta tgcccgcaag
     3481 tgcatgcccg acctggtcct gacgacggac atcatcgtcg gcttcccagg cgaaacggag
     3541 gccatgttcg aagatacgct gaacctgctc aagcgcgtcc agtacgacat ggcctatacc
     3601 ttcatctatt cgccgcggac ggggacgccg gcggccaaga tggaagacca gatccgcagg
     3661 aagaaaagag ccgccgtctg cagcggctca tggatgtgca gaacgtctat agtttagcaa
     3721 aaaataaaga aatggaagac cacgtctatg acgtcatcgt tgaagggccg accaagaacg
     3781 atgccaacca ctggttcggc cggacgacgg gcaacaagat gatcatctgg gaaaatgacg
     3841 gttccgtcgc catcggcgat accgtgccgg tccaggtcga caaaggccag acgtgggtcc
     3901 tcaaaggtca cttagtgcac taaaggaagg tgagactttg gcaggaaaaa aattaacgcc
     3961 tatgatgcag cagtacatgg aagtcaaaga gcagtgccag gataagattc tctttttccg
     4021 cctcggcgat ttctatgaaa tgttttttga cgatgccctg acggcgtcgc gcgaactcga
     4081 cctgaccctg accggccggg ctggcggcaa taaagagaag gtccccatgt gcggcgtacc
     4141 tttccattcg gccgacacct atatcgaacg gctggtccac aaaggctata aagtcgctat
     4201 ctgtgaacag atggaagacc cgaagctggc caaggggctg gtcaagcgca agatcatcaa
     4261 ggtcatcacg ccggggacga ttactctgga gaatgccgtg gcgtcgaaga agaacaatta
     4321 catcggctgc ctggccgaaa gcgatgccat cctttccatc gccctcatgg acgtcacgac
     4381 gggcgaatgt ttgtgggccg actgtgcggc gtcggtcatg gaagattccc tgttcgacat
     4441 cctggccgtc tacgaaccga gtgagctcat ttacgcccac atggggaagg atatggatgc
     4501 cgtccgcgaa tacctgcgca gccacctggc ccagtgtacg gttacggaat ttcctgccga
     4561 tgacagtgtc gattaccggg aacaggcccg gtcctatttc ccgccggaag actttgccgc
     4621 tgcctcggtg gccggcgagt gtatcggcat gctcctggcc tatgtcagtg acgtcatgca
     4681 gtccgatatt tcccacatca attcgctgga acatatcgat aatgaacggc gtctggtcgt
     4741 cgatgcggcc agcctgcgcc acctggaaat cacgcagaac gtccgcgacg gcggccgccg
     4801 ggggacgctc ctcgatgtcc tggacaagac taagacggct atgggcggcc ggctcctgcg
     4861 caagtggctg gaagcgccgc tcatccgcat tgccgacatt acgcgccgcc aggatgccgt
     4921 cgaagatttc gtcctccacg aaatcatgcg ccaggacacg gcagacgtgc tggaccggat
     4981 ttacgatttt gaacgcatcc tgacccgcat cgaaacgggg accgtgagcc ccaaggacct
     5041 ggtagccctg cgggaatcgc tggccgtcat cccgcagctg aaacaggtcc tgtccggtgc
     5101 cagtgccgac ctcctgcaca gcctcaatga ccgcttgcag actcatgacg acgtctatga
     5161 cctgctctgc cggggcatca aggacgaacc gggcctggtc atccgcaacg gcggcgtcat
     5221 ccgcgacggc ttttcgccgg aactcgatga aatccgctcc attgcggcca acagtcatgc
     5281 ctttttgcag gaactggaag agaaggaacg ggaaaagacg ggcatcaaga tgaaaatcgg
     5341 ctatactaag gtctttggct attacttcga gatttcccat gccaatacca agcccatccc
     5401 cgattactac gtgcgcaagc agacgttggt caatgccgaa cgctacatca cgccggaatt
     5461 gaaggaattt gaagtcaaag tcctgaccag ccaggaacgg atgctggcgc tggaatacaa
     5521 gcccttcggc gatatccgcc gccagatcca gggccatatc ccggccatgc aggaaacggc
     5581 ccgggccatt gcctgcatcg actgcctgta cagcctggcc tgcgcggcct atgacaaccg
     5641 ctacgtccgt cccagcctca ataccaaggc gacgatttcc attaaggacg gccgccatcc
     5701 cattattgaa aagtatttga aagacgaatt gttcgtgcct aacgacgtca ccttgaacca
     5761 tgaagaccat gaaatcctgg tcatcaccgg ccccaacatg gccggcaagt cgacgtatat
     5821 gcgccaggtc gccgtgctgg tcctcatggc ccaggccggc tccttcattc ccgccaaaga
     5881 agggtctatc tgcccggtgg accgcatttt tacccgtatc ggtgccagcg acgacatcct
     5941 caccggccag agtacgttca tgatggaaat gaaggaagtg tcgtacatcc tgaaacacgc
     6001 caccagccgc agcctgctca tcctcgatga aatcggccgc ggcacgagta ccttcgacgg
     6061 catgtccatc gcccgggctg tcatcgaata ctgcctgaag cacatccacg ccctgacgct
     6121 gtttgcgacg cactaccatg agctcatcgc catggctgac gattcgccga aaattaagaa
     6181 ttataccgtc gccgtcaagg aacggggcaa gaacatcaag ttcctgcggc gcatcattcc
     6241 cggcggggcc gaccgcagct atggcctgca cgtcgcccgt ctggccgggc tgcccgaaag
     6301 cctgctcaag cgggccgacg tcatcttgga agacttggaa aagaacggcg gagcacccgt
     6361 cccggccaag gcgcctgtga atccggcgcc gtcacccatg ggagacagcc tcttttccag
     6421 tcccgtcatc gacaagttgc tcagcgtcga tgtcagcagt atgacgccga ttgaagcgat
     6481 ttctttctta tataatctgc aaaaagatgc caaagaagga agtgggatcc agtgagttca
     6541 atcattcatg tcttagatga agcaacgcgc aataagattg ctgccggcga agtcgtcgaa
     6601 cggccggcat cgtgcatcaa agaactcgta gaaaatgcca tcgatgccgg tgcccatgcc
     6661 attgaagtgg aaatcgccga cggcggccag tcctatatgc gggtcactga cgacggctgc
     6721 ggcatgagtc cggaagatgc ccataagtgc atcatccgcc acggtacgag caagatcagt
     6781 tccgtcgaag atatttttgc cattacgtct ttaggcttcc gcggcgaagc cgtgcccagt
     6841 atcgccgccg tatcgcacat gcagatcacg acgcgccagg ccagcgatga ctttgcgacg
     6901 cacctcatcc tcgacggggg cgaaatcacg gcagaagacc aggccggcgc accggtcggg
     6961 acgacgatgg aagtgagcga cctcttttac aatacgccgg cgcggcgcaa gttcctcaag
     7021 agcgaacgga cggaaagttc gaaaatctct gaaatggtga ccaaactggc cctggccaat
     7081 ccggccattg cctttacttt taccaataac ggccggacga caatgaagac cggcggcacc
     7141 ggcgatttgc gggaaaccgt tgccaatatc tacggcgcca acgtcgcccg cgatgtcttt
     7201 gccgtcactg ccgaccagga cggtatcagc ctggaaggct atgtaggcaa gccgtcggtc
     7261 ttaaagagca accgcaactg gcagacgtgc atcgtcaatc accgcatcgt ccataatccc
     7321 ttgatgttca aggccattga caatgcctat catgccatgc tgccgaaatc gggctatccc
     7381 tttgccatgc tccacctcca cgtcgacccg gcgacgattg acgtcaacgt ccatccggct
     7441 aagacggaaa tcaaattctc tgatgaacaa gccgtctacc gcgccatcta ccacagcatc
     7501 gtcacggccc tggtggccca ggaaaagccc gaagccatcg ccaagaccat cggtgatgct
     7561 gtaggggccg tcccagaggg cggcccgcag caagtccatg taggggcgcc cacgtggggc
     7621 gcccgctcg
//
LOCUS       DAWABZ010000088         7589 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2606_length_7589_cov_3.288028, whole genome shotgun sequence.
ACCESSION   DAWABZ010000088 DAWABZ010000000
VERSION     DAWABZ010000088.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7589)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7589)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7589
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2606_length_7589_cov_3.288028"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1729 a         1937 c         2361 g         1562 t
ORIGIN      
        1 gaggatatgt ggctttttat ccagaaccag gttttaggga tgcagtggct gaatgaggtc
       61 gttgcggcaa ttttacaata tgccggtgtc gatgtacaga gccgggtcgg cggcagtttg
      121 cagtttttct tttatgatgt cgtgaagatt accgtactgt tatgcgtatt gatctttctt
      181 atttcttata tccagagtta cttcccaccg gaacggagcc ggaaaatcat gggacggttc
      241 cgcggtatct gggcgaatat catgggggct ttgctgggga cggtgacgcc gttctgctct
      301 tgttcgtcca ttccgctgtt catgggattt accagtgccg gcctccccct gggcgttact
      361 ttttcttttt tgatttcgtc acctatggtc gatttaggca gcctggtatt gctcatcagt
      421 atctttggct atcgcattgc cgtggcctat gtggtttttg gcctgatcat cgccgttatc
      481 ggcggttccg tcatagaacg cctgcacatg gaggcggaaa ttgaagattt catccgtcag
      541 gctccggctg tcagtgaagc ggagattcca caattgacaa ggcgcgaccg tttggactat
      601 gcgaaagaac agatgaccag tacgttccgc aaggtctttc cctatatcct gattggcgtt
      661 ggaatcggtg cgttgatcca taattggatt cctgagcagt gggtcgaaga cgttctgggg
      721 cgccagaatc cgcttggcgt aatcctggcc accctggtag gggcaccgat gtatgccgat
      781 atctttggct ctatccccat tgccgaagcc ttgctgggga agggggcgca gttaggcgcc
      841 gtattgagct tcatgatggc cgtgacgacg ctgagtctgc cttcgcttat cttgctgcga
      901 aaagccatca agccgaaatt gttaggcgtc tttatcctga cttgcgtcat cggaatcatc
      961 ttggtcggct acagtttcaa cgcccttcag ggatttctat tgtgaggtga ttcaatgaat
     1021 gccgtagatg tatccttatt atgtaaagcc ttgggggatg cgaaccgcct gcagattgtc
     1081 caattgttga cgcaggggga aatgtgtgcc tgcaagctgc tggagtattt tcaaatcacc
     1141 cagcctacct tatctcatca tatgaaggtg ttgactgaat gcggtctggt gactagccgg
     1201 aaagaatgga agaataccta ttactccctg aattgccaga ccttgacggc tttccgcacc
     1261 tttatagata ctttaaagtg tacgcacact ggctgcgtac cgggcaaagc ctgccagcca
     1321 tcagcgcagg ccgacaaaga ataggagttg atcatatggt aatcaaagta ttaggcagtg
     1381 gctgtagcaa atgtgaatcc ctcctggctg ctgcgaaaga agccgtacag catttgggga
     1441 ttgacgccga agtccaatat gtcacggact ttgcccagat cgcccgctat ggcatcatga
     1501 gtacgccggc actggtcgtc gatgataaag tcgtttcgac aggccgggcc ctcaaagcgg
     1561 accaggtcga agcatacctg aagtaagggg attatactgc aaaaaaaggc gctgtcttca
     1621 tgcgacagca cctttttctg cacttcctag aaccacgtgc gcacgatgaa ccaggacagg
     1681 gccaggatga cgaaggagca ggtgatggcg ctgaccatgg ggcggacgcc ttctttggcg
     1741 atgactgaga agttgacgtt caggcccagg gcggccatgg ccatgcccag gatgaggtaa
     1801 gctatcttga ccagcatggg gacggccaat ccgatggcgg ggatatagga gccgatggca
     1861 ctggctgcga tgaagccgcc catgaaccag gggatgggga ctttgaattt ctgatcccta
     1921 tcctgggact gacggcgctg gcgccaggct tcgacgatgc ccaggatgat ggcaaccgga
     1981 gccaggagca ggacgcggga taatttggcg atgatggcgc tgtctgtccc gaccgggccg
     2041 gcactgccgc cggcggcgac ggcgtgggcg atttcatgga gactcaggcc ggccatgacg
     2101 ccgaactggc tgtccgtaaa gcccaggatg ggctgggcga agacttcgat gagtgtaaag
     2161 accgtgccca ggatggctac gatggcgacg gccaggacgg actggtcggc cttggccttg
     2221 agggcgccgg acacgcccat gacggcggcg gcgccgcaga tgctgcagcc acaggccgtg
     2281 agcagggcca gggtgtgatc gacgcgcagg aaacgggcgg cggcgtaatt gaggggaatc
     2341 atgatggcga cgacaaagac tgccgcttcc agactttgca gaccggcgct gaccaggacg
     2401 atgagattca gcttgaaccc caggaagatg atgccggccc ggaggaattt gttggcaatg
     2461 aagcccgtac tttcccgggc tgccagggtg acgggacggc agacctggat gaccatgccc
     2521 aatagcaggg ccaggacgag atggccgatg agggaaaagc ccggcagggc tgccagggat
     2581 tggccggcca gggaacaggc cagggtcagg acgacaccga gcagggcgtg gcgccagtcg
     2641 atcatagtgg ataaatgcat gatgaagtca ctcctatact gatatttgag atgactccag
     2701 tataatctgt ctctttcaat aagtaaaata ctaggatata atataaatca taagtattta
     2761 cctatgaaat gaggaggaga agtccatgac cttgcgccat ttccgcattt tcctggctgt
     2821 ctgcgagcgg ctgagcatga ccgaagcggc ccgccagctc cacatcagcc agccgtcagt
     2881 cacccaggcc atccatgaaa tggaaaacca ttacggcata ttgttattcg aacgcctggg
     2941 tcgtcatatt tacctgaccg ctgccggcga gaacttgaaa cagtatgcct atcagatttt
     3001 atcgctctgc cagcaggctg aagacagcct gacggccatg cagcatcagc tgccaatccg
     3061 cctgggcgtc agcatcacca ttggcgacat cttcctggtc gaactcctga cgcattgccg
     3121 tcagatacat cctgaatggg acatcctgtc ggctatccac aatacggagg aactggaaca
     3181 gctcctgatg gaagataaac tggacctggc ccttgtcgaa ggacgagtcc attcgccata
     3241 cctcgtatcc cggcccatcc ttacggatta tctgaccctc atcgcttcgc cgaacagcga
     3301 atggagccgt aaggccatta cagcgccgca ggacctggac gacctgcccg tcttcgtccg
     3361 cgaacaggga agcggcacgc gggacctctt tgagcgcgtc ctggaagacc accacctgac
     3421 ctgccgcatc atcggcgtct acaataactc cgaagccatc aagaaagccg tcatggccaa
     3481 cttaggcgta tccgtcctgt cccgccgcct ggttcaaaac gaaatcgaaa cgggccgcct
     3541 cgtcgaactc cctttgccgg gcctcgaatt caagcgccat ttccgcctag tacatcacaa
     3601 aaacaaatac ctgatacagc ccatgcagga cctcatatcc atctgctgcc acagtgatga
     3661 atggctgtga tggaacctaa aaatgaccgc catctgtctg ccgacggagc ggtcattttt
     3721 gattgattta tttcttttcc cgtgtgcttt cgccgacgat gtacggcggg cggcctttgc
     3781 tttcgttata gatgcggccg acgtattcgc cgaggatgcc catgaaggtg aactggacgc
     3841 cgccgatgaa gaggatgagg gagatgaggg acggatagcc tgggacgtct tcgccgaaga
     3901 gtagggtgcg caggacgatg aagacgagat agatggcggc gacgatggcc aggaaacagc
     3961 cggtaaaggc ggcgatgcgc aaaggcgccg tcgtgaagct ggtaatgccg tcgatggcca
     4021 ggttgaagag cttccagtag ttccacttgg tcttgcccgc agcccggggc ggccggtcaa
     4081 agaggatttc tttcttctcg aagccaatcc agctgaacag gcccttggta tagcgctgac
     4141 attcgcgcag gctgcgcagg gccttgacgc agcgcctgtc gagcaggcgg aagtcgccga
     4201 cgtcttcctg gaccgggacg ggggacaatt tcttgagcag gcggtaatag acatggctcg
     4261 accacttctt gaaaaacgtt tccccttcgc ggcgccggcg gcgggccgag acgtcgcggt
     4321 agcctttttc ccaccaggcg atcatgtccg gcaggagttc cggcgggtcc tggaggtcgg
     4381 cgtccatgat gaccagggcg tcgccttcgg cgtagtccat gccggccagc atggctgctt
     4441 ccttgccgaa gttgcgcgac agggatacgt aggccacgcg cttatctttt tcgtggagct
     4501 ggcgcaggat gggcagggtc tggtcgcggc tgccgtcgtt gacgaagatg aaggtaaagg
     4561 cgtagtctgg cagggcgtcg atgacggcgc acgtccgctg atagaactgg ggcaggacgg
     4621 cttcttcgtt ataacagggg acgaggatgt cgatttgctt catcgtttgt cacctccggc
     4681 ggcggcccgg ttcaggagga ccgtatagtc ttcgacgtcg aggcgctggg ccggaaccgt
     4741 tcccaaaacg cgcagggctt cgtcgcggta tttcggctgg acgacgacga tagccggctg
     4801 gtcgctgttc cagaggtcat acaagtcctg gctgccgtag tacatgttcg tctggtccgt
     4861 atgatggatg ccgaattcca gctcgcccat gaagttgccg gcggcgatgg gacgatccag
     4921 atagtagacc aggcctggca tgaacgagcc atacatgatg atcggcgtgc tgtccgggac
     4981 gttcagggac cggatgtcct ggctgactgt ataggccgtc tgatggtcgt gaatctggcc
     5041 ctggatctga tgggcgccga ggccgaagcc ataggccatg cagctgagga tgaccaggac
     5101 cgagaggaag tccttcttgc gatgccagac ccaggccgct gccagggtgc cgacgaagag
     5161 cgaggcgatg agcaggccgc cgtgggtcag gaagtcggcg acggtcagga aatcggtgtg
     5221 catgaggtag gcgatgcccg ctgccgtgaa cagcaggcag aggatggcat ttatgagaaa
     5281 actgtggccg agccagcgcc cttcctgacg gcaccgttcg atggacgcgg ccaggaggac
     5341 cgacaagggc agccagcagg gcaggatgta cgtcggcagt ttcgagtcgg aaatactgta
     5401 gaagacgtaa atcaggccgg cccagagggc gagcagcagc aggtccctct tatgccgcag
     5461 ggaaccgggc ttgcggaaaa tcccgtcttt gctgaagaac gtcgccacga agcccgtcca
     5521 gggcatgagg ccggcgatga ggaccgggac gaagtagtac cagggatgga agcgttcgtg
     5581 catcttggtg gcgaagcgca gaaagtgttc gcggatgaag aagaaataga agaagtccgg
     5641 attcttctgg cagaccaggt agtaccaggg cacggcgatg accaggaaga ggatgatgcc
     5701 cggcaggtag aagagtttga ggaagaggcg cggccgcttg ctcagcaggg cataccagaa
     5761 gaggatgcca aaggggaaga cgatggcgat gaggcctttc gtgagcatgc cgaaacccat
     5821 ggcagcgtag aagacgagca ggtatttctt gttttccgtc cgttcaaaga cgtagaacga
     5881 gaccaggcag gcatcgatga aaaaggtcag ggccatgtcc atgacgccga tagaggcgac
     5941 gatgagctgc agtgccgacg tagccacgat gacggcagcc aggagccctg tgcgctggtt
     6001 gtacatggtc ttggccagcc agaaggccag gaagacgtta ccaaggcccg tcagggccgg
     6061 caggatgcgg cccgtgaatt cacggatgcc aaagagctcc atggacaggg ccgtgaccca
     6121 gtattggaaa ataggcttct cgaaataggg catgtaatcg aagtggggcg tgatgtagtc
     6181 gccggacagg accatttcca tggcgacccg gtgatagcgt ccttcgtcga ggtcgatgag
     6241 atgatacgac ccgatgtggg ccaggtacaa aaaggccgca gcggcgatga acaggcccat
     6301 cagaaagcgc cggtggcggc gaagtgcgtc agtcataaaa tacctcctat atatgtgcgt
     6361 tactatatag tatacaggaa gattataaat aaatcataaa agaaatgaca atttcctgtg
     6421 ttttcatatt tctgtgacat atgtcatttt taccttaatg cgtcagggac aggcgcggcc
     6481 gtctgggttt gacgatgagc aggacgccgc ccaggaccag gagaatgccc agggctgtat
     6541 gccaggtgaa ggcttcgccc aggacgagga taccgctgac gacgccgacg acggggacga
     6601 ccagtatggc gacgccggcc ttgccggctt ccatgtgggc gaggatataa ttccataaga
     6661 agaagccgac agctgcagcc aggataccgt tatagagcag acaggcggca gctgttgttg
     6721 tccagtggat ggaaggctgt gggacaatca gggcgacgat gaagagcagg agcgagccga
     6781 cgaccatctg ccaggccgag tactgcaggg gcggactgtc ctggaggtgc cgtttcagga
     6841 tgatgcccga tgcggcccag gcggcggccc cggcgaggac gatggagacg gctgtcatat
     6901 tgcccaggtc atggatgtcc atgaggacga agaggccggc gatgcagatg gctatgccgg
     6961 ctattttccg gagcgtcagc ctttctttga ggaagagctg ggccagaatg gccatccaca
     7021 cgggcatggt gtagttcaac acggagacga cgccggctgt cagcgaatac atgccgatct
     7081 gcatgcagac attgttgacg gcaatctgca agatgcccgt agcggcgatc cacttccagt
     7141 acttccgttc cgggacggga atacggcgcc aggcgcagac cgagagcatg acggcggcgc
     7201 cgatgaggaa gcggaaggcc acgaacagga cgggcgggaa ataagcattg gccgtcttca
     7261 tgacgaccca gttgaaaccc cagatggcat ataatagaaa tagagcgcgc atcataggaa
     7321 aacaccttct tttttagaaa ttggttagca accttatttt atcggacggg agaggagaaa
     7381 tcaaggcagc ggcccgtccg gcagggcatg gacagacagg ccgggaacgg ctataatgga
     7441 agaaaagatt tacaggagga gcgattctta tgttacatat tggcctgacc ggcggcattg
     7501 cctccggcaa aagtactgtc gtcaccatgc tgcgcgaatt gggggcaggt atcgtcgact
     7561 gcgacgtcat tgcccacgac gtcgtcgaa
//
LOCUS       DAWABZ010000089         7521 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2637_length_7521_cov_3.006831, whole genome shotgun sequence.
ACCESSION   DAWABZ010000089 DAWABZ010000000
VERSION     DAWABZ010000089.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7521)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7521)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7521
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2637_length_7521_cov_3.006831"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4351..4360
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4646..4655
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7160..7169
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1808 a         2088 c         1999 g         1596 t
ORIGIN      
        1 ccctcatctg gtatggtccg atgtacgcca tgcatggcag cgacttcgtc agtcagttca
       61 tcggcgtcca taacgtcctg cgggctaccg tttccgaaca tcctcagttc aatgtctggt
      121 attattacag cgccatcttc ctggccggct tcatcccctg ggtcttcact ctgcccctgg
      181 cctggcgcga ctatggcctg acccggaaaa tcgcggcggc ctggcgggaa aaacgactgc
      241 gcctgcctgc cttgtccatg aagacccagt tcctcatcgt ctgggcggct acgattttcg
      301 tgacctatca gctcatggcg acgaaataca tgacctatac cttcccgtat atgattccct
      361 tggccatcgg ctttgcgtcg tatttgaaga aatatgaccg cctggtcctc aacatggccg
      421 gcatcgtcct ggccatttac gtagccgcta cgtgtacggt catcgtgcct cagtgccagc
      481 aggcttcggc ttatgatgcc gcccaggtcg tcagcggcct ggccgataat gaaacggccg
      541 tcgtcaccta tggcggccgc taccccgtat ccctgaccta ttattccaat catccggctt
      601 atcgcctgga acaggctgat gacattgcca ataaactgcc tggcggtatc agctggaatg
      661 ccaagaacgt catgcccttc atggctatcg aagacctgcc gaaccatcct aaagtcctgg
      721 ccgtcgtccg cgaccgcgat gaagcccact tcctccagga agtaccgggg aactggaaat
      781 ggcgcaccca gaaaggcgtt tgggccatat accaaaaaga agccgatttg tagccctcct
      841 gaaatggcct tgtgactgaa ttgtaacggc tcatgagtat actatgagta acctaaataa
      901 ggagagtgat acttatgaag aagttcaact atatagtacg catgatgctg gttttggtcg
      961 ttaccttcat gaccttcagc ggcgccgccc tgacggccca ggccgcaggc cccagagatt
     1021 atccacagcg ctgggaacag cacgatgacc gcgactatcg tcatgatgac cgcaactggg
     1081 aaaaagaacg gaaagattgg gaaaagaacc ataagacatc gacccagaaa gatgtcgaca
     1141 aagccaaccg taaggccaac atcgccatcg gcgtggccgc cgtcgccacc atcatcgcct
     1201 tgacgaagtg agtgggaata atagtggcta gtggttcatg gctctcctta taggagagct
     1261 gtcagcgaag ctgactgaga ggttgatctt cgttatagtt tgcagtattg agtttgtagt
     1321 ttgtagaaaa ttgctttaaa tttaaaagcc ctcactacaa actacaaact gcaaacttaa
     1381 aacttaaaaa ggggctgtcg cacatgcgac agcccctttt ttcgacgaac gatgaacgac
     1441 ctacgactct aaccgctaac ccctaaccgc taacccctaa cccctagcta cgaaccacta
     1501 gcccctttca attctcgtcc cgtctgttat aatagggagt gcaattagat tctgtttata
     1561 aaggaagtgt ttatatcatg aaattaacgg accgcctggc tgcggctgta tccctcattc
     1621 cacagggggc cgtcattgcc gacatcggga ccgaccacgc ctatatcccg gtctccgtct
     1681 gtgagagcgg ccactgtccg tcggccattg ccgccgatat cgtcccggga ccgctggcag
     1741 cggccgtggc ccacgtccgc ggtgccggct tgaccgaccg catcgactgc cgcctgggtg
     1801 acggcctgac ctgcgtcaaa gcaggggaag tcgatggcgc tgtcatctgt ggcatgggcg
     1861 gcccgctgat ggtgcggata ctccagactt cctatgcggt ctggcagtct ttgaaatttg
     1921 tcgtcttgca gccccagagc gatgccggtg ccttgcgccg ctacctgtat gagcatggct
     1981 ggcacatcga agacgaacgg ctggtcatcg acgacggccg cctttacgaa ctgatgcggg
     2041 ccgtgccggg acaggccggg gccctgccgg actggatcta tgacatcggc cccgtgaact
     2101 ggcagcgccg cgacccgctg ctcagccgca agatagacat tctcatggaa acaaaagggc
     2161 acatcttgga aggcctgaag aagagccgca aagatgtgca cgaacggatg gaaaaaatag
     2221 aaagcgaatt gcgcttctgg agggaattga aatggcaact acagtaggtg aaatcgtcaa
     2281 agtattgaaa gcttgggcgc cgttgtcgct caaagaatcg tgggataatc cgggcctgct
     2341 catcggcagc cctgacgaac cggtcgataa actcatggtc accctggacg tcatgatggg
     2401 gaccgtcgat tatgccatcg aacacggcat ccagatgatc gtcagccatc acccggtcat
     2461 tttcgatggc ttgaaatccc tgcggaccga cacatacagc ggccgcatgt accaaaaact
     2521 cctggcccac cacatttccg tctacagcgc ccataccaac ctggatagtg ccgacggcgg
     2581 cgtcaacgac gtcctggccc gcctcctggg cttgacggat ttgaagggcc tcgtccccgt
     2641 cgccgaagat aaactctata aaatcgccgt ctacgtcccg gaaagccatg gtgacgccgt
     2701 ccgccaggcc ttggccgacg ccggtgccgg ctatatcggc aactacagcg actgttcctt
     2761 tacggccaaa ggcgaaggcc gtttcaaagc ccatgaaggg acgcatccct ttatcggtga
     2821 aatcgggcaa gtcgaaaaaa cggcagaaga acgggtcgaa accatagtcc ccgaaagcaa
     2881 gctccgtcgg accgtacagg ccatgctggc cgctcatccc tatgaagaac cggcgtatga
     2941 cctgtatccg ctgaaaaatg ccggtcatcc cttcatgatg ggccgcgtcg ggacctggcc
     3001 gacaccggaa ccggctatgg acgtcttgaa gaaaatcaaa ggcctccttc atcgcgatgc
     3061 cttgtcctat gccggcgata cggacgtcat cgtccgccgc gtcgcccttc tcggaggtgg
     3121 cggagctggc ttcatcaaac tggcgagaga cgccggtgcc cagctttacc tcacaggtga
     3181 tgtcaaatac cacgacgccc aggaagccat ccgacagggc atcgtcgtcg ccgacggcgg
     3241 ccacttcggc accgaatcgc ctgtcgtcgc cgacctctgc cgacgcctgg aacaagctgc
     3301 cgaagaacag caatggcaca tcacctgcga aaccgacccg accagtaagg acatgctgca
     3361 ttatatgtaa agcgccttat ggcgcgccgt tcgcaggtcg caggccgcaa tggctcccct
     3421 gataggggag ctgtcagcga agctgactga gaggttgatt tcctgtcaga tagtgccgct
     3481 gtgaaattca accacagccc attcttgtcc aatggcgggt ttttacgagc cacgagccac
     3541 gagccacggc cttccaaggc gaccagcgaa ctgcggggcc gtcaggcccc attaattccc
     3601 atagaaagga aacgtatcat gactaaagaa gaacatgtaa gacatttatt agaagacccg
     3661 gtatatgcca ttatgggcaa cgaggaactc tgcctgggcc gtagcaatat ggacgtcgcc
     3721 aaggaattga tcgcggctgg cgtcacgatt atccagtacc gcgaaaaaca taagaaatgg
     3781 cgcgccaagt acgaagaagc caaagccatt gcccagctct gccacgacca cgacgtgacc
     3841 tttatcatga acgactcgac agacctggcc atcgcctgcg gcgccgacgg catccacgtc
     3901 ggccaggatg atgcaccggc ccagatcgtc cgtcagctcg ccggcccgga cgtcttcatc
     3961 ggtgtatcga ccaataccgt cgatgaaatg aaacaggccc tggctgacgg tgccgattac
     4021 gtcggttttg gtcccatgtt cccgacacag tccaagaaag acgccgacga agtcgtaacg
     4081 cctgaagcca aggaatatgc ccttaaattc gacctgcccg tcgtcaccat cggcggcatc
     4141 agcctggcta acatccgcag cctctacgac gaaggtttcc gttcctttgc catgatttcc
     4201 gccatcgtag gccaaaaaga catcaaaggc gccgtagaaa aaatccgccg gaccttgcgt
     4261 tagctataag cgaatagagc ttatagcgcc ggtcgcagtt cgcagaccgc cgtccgcaat
     4321 ggctcccctg ataggggagc tgtcagcgaa nnnnnnnnnn gatagtgccg ctgtgaggtt
     4381 caaccacagc ccattcttgt ccaatggcgg ggttttacga gccacgagcc acgaacaacg
     4441 agccacggct ctttcctcgg atttagatgc ggcagttctg ccgcgacaga ctcatgattg
     4501 tattacgacc atgtatcacc tgtcataaat gccggctgtg cccgagattc acggcggcct
     4561 gcgacctgcg gggccatcag gcccctgatt cgatgaacgg aatcggcctg cgggcccgtc
     4621 aggcccttga ctctcatcat caaatnnnnn nnnnnaaata tggtatccta tacgaatgat
     4681 gagtatgaaa agcaatcgct agtgcttttg cattagagga aagtccgagc tccgtagggc
     4741 aagatgccgg ctaacggccg gcgaaggtga ctttagggat agtgccacag aaatgtgtac
     4801 cgccacgcaa gtggtaaggg tggaacggtg cggtaagagc gcaccagcgg tgcagtaatg
     4861 catcggctag gtaaacccca tctggagcaa gaccgaatag gggagggatg aagcggcccg
     4921 ctgatcttcc gggttgtgtc gcttgaacat gctggcaacg gtatgtctag atagatgatt
     4981 gctgctgcgc aagcagaaca aaactcggct tactgattac tcatcacctt gtgaatctaa
     5041 aaggactgtt acacgaaggc ataggccatc gtggacagtc ctttttttat gtttgaaaac
     5101 ggccatttca tcacgatgaa cgaagaacga cctacgatga acggacggcg gcctgcgacc
     5161 ggcgaactgc ggcctgcggg cctttaggcc ctacaagccc tttacatttc cccttgtcca
     5221 tgatatcatt tcaataagtg gtttcagatg gtaataaacc ataataatga attaccatga
     5281 taatgaatac ttaaacaaac aaaagcgagg gatttaagtt ggcattaatc gttaagaagt
     5341 tcggcggcag ctccgttgcg acaccggaca aaatgcgggc tattgtacaa cgagtcttaa
     5401 aaggcaaaaa gcctgaagac aaaatcgtca tcgttgtttc ggcaatgggg gatactaccg
     5461 acgatttagt gacattagca aagcagatta cctctaagac ttacggccgt gaaatggata
     5521 tgctcctgac gacgggcgaa caggtctcca tcgccctcat ggctatggct ttccaggaag
     5581 ccggccagaa ggctatttcc tttaccggtg ctcaggccgg catcaagacc agcgattcct
     5641 ttaacaaagg ccgtatcctg gcattgaaac cgggccgcat catgaaagcg ctggatcagg
     5701 gcaatatcgt cgtcgtcgct ggtttccagg gcattacggg ccatggcgat atcacgacgc
     5761 tgggccgcgg cggttccgat acgacagccg ttgccattgc cggtgccatc catgctgatg
     5821 tttgtgaaat ctataccgac gttgacggcg tatacacgtc cgatccccgt gtcgttccca
     5881 atgcaaggaa aatcgaacag attacatacg gcgaaatgct ggaaatggcg aaactcggcg
     5941 ccggtgtcat gcagccccgt gccgtcgaaa tgggcagccg cttcggcgtc cccatccatg
     6001 tacgctcgac tttttctgaa gatgaaggaa ctatgataca ggaggattgc tctatggaag
     6061 tcaaacagta tctcatccgc ggcgtcgctt cggataaaaa tgttgcaaaa atcaccgttt
     6121 tagggattcc caataaaccg ggcttcgctt atcgcgtttt cgctgaattg gcagaaaaac
     6181 acgtcgacgt cgacatgatc gtccagagtg tccgcattgc caacgaaggc gtaacggata
     6241 ttacctttac cattgcccgt acggacctgg aacaggccaa agctgtcttt gccaatctgc
     6301 gcaaggaaat gcctgtcgaa gatatcctca tcgatgataa gatggctaaa gtttctgtcg
     6361 tcggtgctgg catggctggt catccgggca tcgctgccgg catgttcggt gtccttggcg
     6421 ataacggcat caatatcgaa atcatttcca cttcggaaat cagcatcacc tgcctgattg
     6481 cagaagatgt catcgacaaa gccgtcaaag ctatccacgc gcacttcttc gacgaagact
     6541 gagggggata catgtctgta ttggatagct ttacagaaga gatgttgcag tctgaactgc
     6601 ccaaacgggt catcctcgaa cgcctgaccc atggcctgga agtagaaaag ccgccgcagt
     6661 ttgccattcc ggctcccaaa tacacctttg agaccaatct gcacggcttc cgttacgact
     6721 atcaacatca gaccgtcacc atttcctata aagtagccca cggccttcat gacgatatga
     6781 ccgtatcctt tatgactttc cgggtcatcc tggaaggatt gggcgtttgt atccgaatgc
     6841 agaagtggta gtgaaataca gagggggttg tcgccaatgc ggcagcctcc tttttgtggt
     6901 gaaggagggg aaacgcggtt cgcaggccgc agatggcagt ccgcaatggc tcccctgata
     6961 ggggagctgt cagcaaagct gactgagagg ttgagaggca tgcgtagggg ccgtcaggcc
     7021 caaaaaggag gggaaacgcg atgcattgcg gaaccgatat cgtacaaatc agccgtatag
     7081 cggacatggc cgcccgtcac ggccgttcct tgggccgctt ttttacggaa caggaactgg
     7141 cttattgcca gcggaacgan nnnnnnnnng catctttgct gccaaagagg ctttgttcaa
     7201 ggccttgagg accggtttcc gccagggaaa atggacggac gtcgaagtct gccatgactc
     7261 gttgggagcc ccgtattatc agctccatgg ctattacgcc caggccgtgg gccaggatgg
     7321 ccagctgccg tccctgtcca tcgcccacga cggcgattac gccatcgcct tcaccgtgtt
     7381 agggtaaaaa aaacgcagtc cgcaggccgc agatggcagt ccgccatggc tcccctaata
     7441 ggggagctgt cagcgaagct gactgagagg ttgattggct gtcagatagt gccgctgtga
     7501 ggtttaacca cagcccattc t
//
LOCUS       DAWABZ010000090         7417 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2684_length_7417_cov_2.603369, whole genome shotgun sequence.
ACCESSION   DAWABZ010000090 DAWABZ010000000
VERSION     DAWABZ010000090.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7417)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7417)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7417
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2684_length_7417_cov_2.603369"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    964..973
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1316..1325
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3171..3180
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1751 a         1847 c         2088 g         1701 t
ORIGIN      
        1 gtcttgtacc tcaagaaagc cggtactttc atcctcggcg catccgtcct cgtctggttc
       61 ctcacggcat acccgatgga cgtcgaatat tcccaggact tcgatgccgc taaaaatcag
      121 gtcacggctg aaatggaaca gaaacagtct gacatccttc agtcctacgg cctcagcgct
      181 atcgaagaca atgccgcttt gaacgatatg tatgaatcca tggtcgctgc cgctgatgaa
      241 gccgctgacg aagccgatgc tgacgaagcc gatacgggca atgccctgaa cccggctgcc
      301 gctttgggct ccgttgaaga ccaggtcaag aattccgaag aagaagaaga caacttgttc
      361 atgggtaaat acccccagag cttcgccgac ctgcaggaac agaatccggc cgtcttcgcc
      421 caggctctgc cgctcttcga tgccaaagcc gatgctgacg acgaaacgtc caaactcgac
      481 gaccagcaga cggctgaaaa actggaacag tcctatgctg cccgcctggg gcacttcgta
      541 gaaccggtca tcgcaccgct tggctttgac tggaagatcg gcgtaggcct catcgcctgc
      601 acggctgcta aagaagtcat ggtttcgacc ttgggtacga tttacagtgt cggcggtgac
      661 gacacccacg aatcgggcat cgtcgcttac ctccgtgatg acccggactt caacccagcc
      721 gtcgccctgt cgctcatggt cttcgtactg ctctacatgc cgtgcgtcgc tgccatggcc
      781 gtcatcaaac gtgaaacagg ctcgtggaaa atgctccttg ccagcgatgc catgtgcctc
      841 gtactctcgt acgtcctggc ctttgtcacc tatcacctgg ccctcatggc cggcctcggc
      901 gcttaagact tgaagcagcc ccttccataa cgtataatat tagtggctcg tggctagcct
      961 cccnnnnnnn nnntcatagg ccctttttgg cccgtcaggc gttgaccgca gggaaaggca
     1021 ggacggctgc caaaacggag agccggctgg aggtggccca aagggccgga gaggttgatt
     1081 tcacaacgaa taattgctaa tcccgagagg aggtatatct atgagtcctg caaccattat
     1141 cgtcgctatc gtcgtcatca tcgccgtagc gtacttcatt caccatatcc gcggcctgtt
     1201 caacggaacg gccagctgct gcggcaactg ctcaggcagc tgctcatcca gtggaaaaac
     1261 aaaaacgggg gacgattcca aattgcctcc gtgctgccgg aaatagcata agaaannnnn
     1321 nnnnntgtta gttgttagcg gatgggttta aatttaaagc ttttttctgc aaactacaaa
     1381 ctctgtgcta caaactataa agtaaacggg gcatcacatg atggcagaaa ccttcgtgcg
     1441 acagcctctt tttgctttcc gcctgctgcg acggcaggaa acatggtata atgaaagaaa
     1501 agtatcagaa aggatgaagt ccatgattaa acgttgtaac catgactgtg atttttcctg
     1561 ccagcagtca aaaaaacaga aggtgcgagt catcggcggc accggcgtct ttacgctcct
     1621 cgtgtcggcc caggtgcgca gtgagaacgg gaagtaactc gaatgaagga catagcatga
     1681 aatctattgg ttcaataatc aaagagaatc tggtactttt tctggcagtg gcagcggcgc
     1741 tggcgtcgat gacggccgta ccgccttcgg ctgcttatgc cgggtatatc aatatgcctg
     1801 tactggcgat tttgttctgt ctcatggtcg tcatcggcgg cgtgcgggaa agcggcgtct
     1861 tttctgccgt gctggcccgt ctcctgctcc gcctggggac gatgcgccag ctggcggcgg
     1921 tcctggtatt tgcctgtttt tttgtcagca tgtgggtgac caatgatgtg gccctcctga
     1981 cctttgtgcc tttttccctg atggccctgc ccaaggtggc cagtgaggaa cagatggctc
     2041 ttgtcatcgt cctccagacg ctggcggcga atctgggcag tatgtgtacg cccgtcggca
     2101 atccgcagaa cttgtatctt tatttttatt acgctatgga tttgccatcg tttctgacgc
     2161 tgctcctgcc atataccctg gtgtctttcc tgctgcttac gctgtccctg ttccgcttgc
     2221 cggggacgcc gattcccatt tccgtgcggc tagcggcacg gcggacacga aatctgcctg
     2281 tattggctcc gtcgcggcag ctctggattt tgggcgggat gtttttcctt tccctgctcg
     2341 ccgtggcgca tgtgctcgac tatcgcctgg ccctggcctt tgtcgtggtc gtcacggcat
     2401 tgacgcggcc acgatatttc cgttatgctg attaccggct tttgggtacc tttgtggcct
     2461 tttttatcct cgtaggcaat atgagccaga tgcctggatt cagggacctg ctgacagggt
     2521 atctggacgg tcatgaattc ttcatggccc ttttggcaag ccaggtcatc agtaatgtgc
     2581 cggcagccgt cctcctgtcg ggctttaccg atgaggcagc ggccttgttg ctgggaaccg
     2641 atataggggg attggggaca ctggttgcct ctatggcaag cctcatttcc tatggcatgt
     2701 acgtccggtc ctatccccgg cagcagggaa ggtatctgaa actgtttggc ctgctcaacc
     2761 tggcctatct gctggtactg gtcctgattg cctggctgta cggattcgga gtataataaa
     2821 aatatagaat taggaaagaa taggataagg agggattttc catgagtgta gaatttgaag
     2881 gaaataaggc gtgcattgcg ccggaagggg tatttatcat cgggacgtac gacgaaaagg
     2941 gcgtgcccaa tgccatgaat gccgcttggg gagcccaatc ggactacggc gaaatcacgc
     3001 tctttttggc taagcacaag acgacggata acatcaaaca taccaaggcc tttaccgtcg
     3061 cctttgccac caaggatacg gttgaaatag ccgattactt tggcgtagaa acgggaaaca
     3121 acgtgaataa aatcgaaaaa gccgcctgcc atgtccacaa gagtcatcat nnnnnnnnnn
     3181 tcccagtcac cttggaatgt gaatgtacca gctatgatga caatacgggt atccttgtag
     3241 gcaaaatcgt agctcagcag gccgacgaat cggtcctgac agacggcaaa atcgactacg
     3301 acaaactgca gcccatcatc ttcgacatcg ctaccatgac ctatcgcctg atcggtcccg
     3361 tcgtaggcca ggccttccat gacggactga aattgaaagg ctgaacttag cgtatccgta
     3421 aaccagtttt ttgcaggtgc tccgttaggc atttggatga ttccccgggc aaactgagct
     3481 ggcaatcgta catagactgg agcgggcgcc ccacgtggcg cccctacgaa ccatggcttg
     3541 ggattgtatg ggcgtgctgc ggctcggtct ggagagcttt aaatttaaaa ccatctgcta
     3601 gccgctagcc gctagccact agccacttaa aaaggcgctg tcttcatggg acagcgcctt
     3661 tttcctaatg tcctgcgact tcggctgtgg gctggaatgc ctgggcagct tcggggtttt
     3721 cgttcatggc gtcgatgcgg gcgcggtatt cgctatccga agcgtagcgt ttgtttttgt
     3781 aatagccgat ggtcaggaag gggacgatga tgatggcgat ggctaagtaa ccgcaatagc
     3841 cgtagccgta tttgatgatg ttggtcaggc cggcgacgga gacggtcatg gaagcgatga
     3901 tgatgaataa ggtaacgata gccgaacggg tgacggcgtt atggcttact ttcttcaggc
     3961 atttcgtttc cgagaagcgg gccgtgaaac cgaagacggt tgtgacgcct gtcgaaatga
     4021 ggcacagcag taagaaggcg ctgtaggcga tggtcagcca ggacaggccc atggctttgc
     4081 aactagtcag ggtcggtaag ggcgatttgc cgtagatgcc ggtccagcct aagagcatgc
     4141 agacggctaa ggtcagggcg atggcgttca tagcgaagga gaaccacatg gacgtggcgc
     4201 aattcttgcg ggtgactaag ggcgtgccga cgacgatcat tgtcgggatg acgacgcact
     4261 ggaaaccgga ataggtgaag gcatggagaa tggctgtcgg tactttactg aagccattcg
     4321 tggcgaaatc ctgggccatg gcagccgtga agaactgcgt cccttcgatg gaaccgccca
     4381 tatagatgcc gacggagaaa atcatgatga ccatggctaa gatggccatg cccatatagg
     4441 tcgtggcccg gcgtaccaga ccggcgccga agatggtcag gaagaagatg atggtgccga
     4501 cgatgccgat accggcgtaa taattgaggc ccgtctgagc gttgatggcc gaggcagccc
     4561 cggaaatcgt agccgatacg gccattaaga ccatgatata gaagaaggct tcaaagaggt
     4621 attcaatgcc tttgaagggg ctgtataagt tctggaataa ttgacggtaa ctggtgagat
     4681 gacgggaatt gtacatgttc atggcttcct tgatggtcag cgtgaacagc agcatagtga
     4741 tgatagccgt aatcgggccg agccagccga ggctgacgaa gtagacgttt tcctggttgc
     4801 ccgtagcgaa accaccgccg gcgtgggaac tgaataagac ggcaccgacg gaaaaacaca
     4861 tgctcagtga agcggctttg atcgatgaaa tattagccat aagattttcc tccttttaag
     4921 aaccatggag agaaacgaaa acgcctttcg tctctctgct cgaagcatta agagacgaaa
     4981 ggcgttgtat gatccttccg cggtaccact ctgtttcatc cctggggatg cactctcgga
     5041 tacggctcat ggcgatatcc acgctctgta acgggagttc ccgtatcggc ctactccatt
     5101 cagcctctcc gctcggcaat gagttcggtc cggcatcgcc gctgcctcac accagccggc
     5161 agctctctgg aaacgataga ctcagacgta ctattttgcg tcgtcgcgtt tatgacgttt
     5221 atcagttgat ggtgctatga tagcatcatc acttaataaa gtcaaggatg aaacataata
     5281 ttttttgaaa taaacgcaat aaacgtttaa gtttaaggtt taaccataaa atatagtttg
     5341 tagtactgag tttgtagttt gtagaagatg gttaaaaaac ttcactacaa actacaaact
     5401 gcaaacttaa aactcaaaaa ggcgctgtct tcatgggaca gcgccttttt cctacttatc
     5461 gcgggaaagg tcgacctctt tcaagggaat gaggtgagtc ttatgtttga gtttgtgata
     5521 gaagaagacg gccaggaaga cggggatgcc gatgtaggcg acactggcac cgtaccagtc
     5581 gatttctgcg cctgtgaagg ccgagtagtt ctggcctgcc gtgacgatga tgcacatgat
     5641 cagggccagg agggggccga aggggaacca cgtagcctta tagggcaggt ctttcaggtc
     5701 tttgccctgg gcgatatagg cccggcggaa gcggaagtgg cagatggcga tgccgatcca
     5761 ggtgatgaaa ccggccatgc cgctgatgtt gaccagccag tcataggctt tgccttcgcc
     5821 gatgagggac gtcaggaagg cggccatgcc gaagacgacc gttgcgaaca gggcatacga
     5881 cgggacgccg cgcttgttca gtttgaggaa gcatttcggg gcctgtcccg tttcggccat
     5941 ggcgtagagc atacgcgtcg agacgtagag gcccgagttg cctgccgaca ggacggcggt
     6001 caaaatgacg gcgttcatga tggaagcggc tgcggccagg ccgaagcgtt caaagaccag
     6061 cgtgaacggg ctgatggaaa tgttttcgat gttcgtattg agcagattcg ggtccgtgta
     6121 ggggatgagg aagccgatga ccgtaaaggc acctaaatag aagatgagga tacgccagaa
     6181 gatggagtgg acggcgcggg gcacgttctt ttccggatct tcactttcac cggcggcgat
     6241 gccgatcatt tccgtcccct ggaaggagaa gccggcgacc atgaagatgc ccagcatggc
     6301 cgtgaagccg ccgacgaagg gcgcatcgcc gatggtccag ttggtaaagc ccggcgacgg
     6361 ttccggcccg aggcccagga tgagcaatgt cccggcaaag aggaagacca ggaccgtcag
     6421 gaccttgatg ccggcgaaaa tgaattcgct ttcgccgtag gaccgggtcg acaagtagtt
     6481 gaggacgaag aggataatga ggaacaggcc gctccagatg gcagccggta cgtcagggaa
     6541 ccagtatttc ataatcaggg caccggcgac aagttcggcc gctaccgtga tggcccagtt
     6601 gaaccagtag ttccagccca gggcaaagcc cagggattta tcgacgtagc ggttggcgta
     6661 ggtttcaaaa gaaccgctga ccggcaggta cgacgccatt tcgccgaggc ctgtcatgag
     6721 gaaataaacc atgataccga tgagggcata ggcgacgagg gcaccgcccg gaccggcaga
     6781 gctgaccgtt tcaccgccgg cgacgaacag gcccgtgccg atagcgccgc cgatggcgat
     6841 catgttcatg tggcgggctt tcaggctgcg gcgcagtccc tgctgctgtt gagtttcttc
     6901 catgcaaaat cacaaccttt ttttcattga attgaattga taaataaaat ctaaaaaaac
     6961 tcataccctt acattataaa ggatgaatcc ttctctgaca aatggatatt ctgcatggaa
     7021 gtaaatcgtt tatgcgatta atggatatat aacgtgacgc tgtagtcttg acaaggattt
     7081 cgagaacagg tacactaaag ataaaatagt tgaacatgtt caagaaggtg tttttatgga
     7141 aaaagataaa gacgaagtcc gtcagcgcat cctggctgcg gcgaaggagc tctttgtcca
     7201 gcaggggtat aaaaagacga cgatccgtca gatcgtcgag aaatcgggcg tcctcacagg
     7261 gagcatctat tatttcttca agaataaaga agatatcttc cagtccctgg tcatggagct
     7321 gctgcggcag tgtaccgatt tgattgcagc ccatttcagg caggagtcgc cggccttccg
     7381 ctacgccgcc ctgtgcctca tcgaatttga agccgtg
//
LOCUS       DAWABZ010000091         7345 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2718_length_7345_cov_2.995199, whole genome shotgun sequence.
ACCESSION   DAWABZ010000091 DAWABZ010000000
VERSION     DAWABZ010000091.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7345)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7345)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7345
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2718_length_7345_cov_2.995199"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1697 a         2023 c         2085 g         1540 t
ORIGIN      
        1 aaatgagcaa aaaaactcat tttgttacag cccctttttc tagaaaaatc gtccgataag
       61 gggcattttg gctgcgtctt ctttggtgac accgccgttg aggaccatgc agccgatgta
      121 gatggccgac ccgacgatgc aggtcaggaa gaggtccagg aagagtccca ggtaagggaa
      181 gaggaaggcg tacatgaaat acatggccac gcccatgatg gcggcagaga cgatgttttt
      241 catgacgact ttgatgtcga taacgtaacc gattttacgg taaatgaaga acatgttgag
      301 gacggcagcg acgccgatgt cggccactgt cgcataggaa gccccggtga tgcccagggt
      361 cgggatggcg actaagaacc agttgcacag gaccttggcg acacaggcga cagccatgtt
      421 gatgaccggg atcttcggtt tcttcaggcc ctggaggata gccgtcgaaa tctggtgcag
      481 gcccaagaag cagatggcga tggccacggc ccgcgtggca tccgtagcgg ccggcgcgtt
      541 gtagatgaag gtgacaatcg gttcggccat gacgtagagg atgacgctga agggaatggt
      601 gaccaggaag gagatgcgca tggacgtcgc cgtccgttcg cggatttcat cgtattcctt
      661 caaggtgaaa ctgtggctga tggccggcac caggctcatg gccatggctg ccgtcaggat
      721 cgttgccagg ttgatgagcg gtacggacat gcccgtcagg taaccgaaca gctccgtggc
      781 ctgatgggtc gaataaccgg ctacttccag ccgccgcggt acgatgagca ggtccaggtt
      841 ggatacgacg gggagcatga tgctcgacag ggaaatgggg atagccagga tgaccaggcg
      901 cttgaggatg tgcttgatgc tttcctgctc gaatgtcggc ccgtcgacgg ggagggtccg
      961 cttcaactta taatagtact tataatagta atagaccagg accagccagg ctgcaaaccc
     1021 accggcgccg gcgccgagac tggcaccgcc ggcggcataa tcgaggccgt acggcaggag
     1081 gagcgcggcg aagccgagca tgacgacgac gcggaccagc tgttcaacga tctgggaaat
     1141 ggctgtcggt gtcatctgct gccagccctg cagatagccg cggtagccgg caatgatcgt
     1201 cgtaaagaag atggccggtg ccagggcgat gagcgaataa taggcccggc tttcgcggat
     1261 gatgtgttca tcgatgagga agctggaacc aaagaacatg aggatactca aaaaaagtgc
     1321 cgtcgtacag agcatggaca aggacacgtg gaaaatgcgc tgggcgccga aatagtcctg
     1381 tttggctact ttttcagctg taatgatgga aattgcaatc ggcagcccgg cactggaaat
     1441 atcgagtgcc agcaggtaaa tcgggaaagc catctgatag atgccgatgc cttcaccgcc
     1501 gagaatacgg gacaagatga tccagttgat actgccaatg gctttgacga caaagcccga
     1561 caaggtcagg atcaatgtgc cggagataaa cttgtttgcc atgataaccc cttaactcta
     1621 taaaattttt accaacataa caactaattt tagcacattt gccagacctt gtatatagtg
     1681 aagtgttagc cgttcgtggg ggccgtccca aaggggagtc agccgatagc cacgtattcg
     1741 ccattattca tggcgggcgc cccacgtggc gcccctacga gccacgagcc acgaaccacg
     1801 agccacgaac cactagtcac gacctgcggt ctgcgctctc aaaatatcca tgaattcttt
     1861 gccggtaatc gtttcttttt cgtagaggta tttggccagg gcatccaggg ccgggcgatg
     1921 gtcttcgagg attttcttgg ccttggcgtg ttgttccttg acgacagaga cgactttggc
     1981 gtcgatgtat ttctgcgttt ccggcgcgca ggccagggac gtatcgccgc cgaggtattt
     2041 attggtgact gtttccatgg cgaccatgtc gaattcgtcg gtcatgccgt aacgggtgat
     2101 catggcccgg gccagcttgg tcgcctgttc gatgtcgttg gaagcgcctg tcgtgacctg
     2161 gttgaagacg acttcttccg cagcccggcc gccggtgaga gtggcaatct tgttttccag
     2221 ttcttcccgg gacaggatgt atttatcctg ctggtcgacc tgcatggtgt agcccagggc
     2281 gcctgatgtg cgggggatga tggtgatttt ctggaccggt gccgaatggc tctggagggc
     2341 agcgaccagg gcgtggccga tttcgtggta ggcgacggtc ttcttttcgt cgtccgacaa
     2401 gatggcgttc ttcttctggt aaccggcgat gacgacttcg atgctttctt ccaggtcggc
     2461 ctggctgacg gcgtcgcggt ggtcgcggac ggcgcgcagg gccgcttcat tgacgatatt
     2521 ggctagttcg gcgccggaag ccccggcggc catacgggcg atagtgtgga agtcgacgtc
     2581 gtcgccgagg gcgactttct tggcgtgggc cttgaggatg gcttcacggc cagccaggtc
     2641 cggcagttcg acagggacgc ggcggtcgaa acggcctggc cgcagcaggg ccgggtccag
     2701 cgattccggc cggttcgtcg cggccaggat gacgacgttg tcatcggcgt tgaaaccgtc
     2761 catttccgtc agcagctggt tcagcgtctg ttcccgttcg ctgttgccca tggcattgcc
     2821 cgtgcgtttc tggccgatgg cgtcgatttc gtcgatgaag atgatgcacg gcgccttctg
     2881 cttggcctgc tggaagaggt cgcggacctt ggaagcgccc atgccgacga acatttcgac
     2941 gaattccgac ccggcaatgg ggaagaaggg cacaccggct tcaccggcca cggccttggc
     3001 cagcatggtc ttgcctgtgc ccggcgggcc gacgaggagg acgcctttgg gcatcttggc
     3061 cccgattttg gcgtatttat caggatgatg gagaaaatcg acaatttccg acaggttttc
     3121 cttggcttcg tcttcaccgg cgacgtcatc gaaatggatg ccgttagccg acgggacgta
     3181 gaccttgacc tggttcttgg cgccgaacat gagcgaattg ccgccgccgt ggttcatgag
     3241 cttgcggttg aaatagtagc cgacgccgaa gaaaatcagg atcggcagga tccaggcgat
     3301 gaggaagtcc gtaaagggcg aatccttttc cttgatgact tcattgaagg agacattggc
     3361 cttgtgcagc cgttccgtca gctgcgggtc gttgacgacg cccgtgcggt attcgttgcc
     3421 gtccttgtcc ttgaagagaa tctggttctc ttcgatttgg acgtcggcga tcttttcgtc
     3481 ttccgtcatg ttcatgaacg tcccgtaatc gacctgtttg acactcttcg aatcataata
     3541 aggccgggcg atggcgttga acatgaaaat gacagccaaa gcgacgatat aatagaataa
     3601 aatcggtttc ttcttttctt tgacttcttt cacaattcct cacatccttt gaaaaaatca
     3661 agctatactt ttattgtaca ctccttgcgg gacctggcaa gaggggactg ccttgcatat
     3721 tcccggtgga tgtgctaggg taaaagaaaa tacaccaaga aagtgatgaa gcatataatg
     3781 aatatattaa ccgtagaaaa tgtaaagaaa tcctatggcc tgcgcgtcct cttcgacgac
     3841 gtgacctttg ccatcgacgg cggccagaaa ctgggcctca tcggcttcaa cggcgccggc
     3901 aagacgacac tcctgaaaat cattgccggc ctgaccgaca ccgacagcgg cacgatctgg
     3961 cgcaatccca aggtgcgcat ccattacctg ccgcaggaac cgcagctcgt gtcgggccat
     4021 acggtcctgg aaagcgtcct cgacggcgac ctgccggtca tgaagctcct ccagtgctac
     4081 gaagaagcgg tccagcgcga tgacgatgca gccgtcgtcg ccctcagtgc cgaaatggac
     4141 gaacagcagg cgtgggaact ggaagcccat gccaagatca ttctcagtaa gttgggcatt
     4201 tccaattttt cccagtccgt cgacgaattg tccggcggcc agcgcaagcg cctgggactg
     4261 gcccgggccc tgatcatgcc ctgcgacctg ctcatcatgg acgaaccgac gaaccacctc
     4321 gacgacaaga ccatcatgtg gctcgaagaa tacctgcgcg acagcaaggc cgctatcctg
     4381 ctcagcacgc atgaccgcta tttcctggac tgcgttgccg attccatgat cgaactcgac
     4441 cggggtcacg tctataccta taccggcaat tacggccagt acctggaaca gcgccaggcc
     4501 cggctcgaac gggaagaagc gagcgaagcc aagcggcgca acctcatccg ccgtgaaaag
     4561 gcctggatcc agcgcggcgc ccaggcccgg tcgacgaagc agaaagcccg taaggaacgg
     4621 tatgaacagc tctgcgccat cgaagcgggc aaacccatcg gcgaagtgga aatgttcgac
     4681 acgtccgtcc gcctgggcaa gaccatcatc gacatcgacg acatggcctt cggctacgac
     4741 ccggcccatc ctttgttcca gcacgtgacc tatcacgtcg tcaagcacga ccgcatcggc
     4801 atcatcggcg agaacggcgt cggcaagtcg acactgctca aagtcctggc cggccagctg
     4861 gcgccgacgg aagggacagt cactatcggc cagaccgtgc gcttcggatt ctttacccag
     4921 cagctgcccc agttcgacga aggcatgcgg gtcatcgact acgtccagga acacggccat
     4981 ttcgtgacca accagttcgg ccagcgcatt tcggcgacgc gcctgctgga ccagttcctg
     5041 ttcactgaag aaatgcagta tacctacatt gccaagctga gcggcggcga acggcggcgc
     5101 ctgtacctgc tgcgcctgct catggaccag cccaacgtcc tcctcctgga tgaaccgacg
     5161 aacgacctgg acatcccgac cctgaccgtc ctcgaacgct acctcgatac tttccagggc
     5221 gtcgtcctcg tcgtatccca tgaccgctac ttcctggacc gggtggttga taaacttttc
     5281 acattagaaa aggggacatg ggaccgcttt tacggtgact atagtgaata cttggaagaa
     5341 aaatacagtc atcaccaaga aaagaaaagc gaaaagcccg tttcaccttc tattactaaa
     5401 acgaaagccg gcctgacgtc gcggcaggaa gaagaattgc ggcgcatcac ggaagaactg
     5461 ccgcgctacg aagccatgct caaaggcctc aatgcggcta ttgccgccgc cgggagcaat
     5521 tacgaagaag tcgaatccct gctggccgag cagaaggaaa cgcaggataa agtcgatacg
     5581 ctgaccgaaa gatggtgtga gttagaagac ttaaaatcat agattacggc gattttttta
     5641 aaatattaca aaaaagcata gaaattatgg aaaaataggc agatgaatta cacaaaagta
     5701 atggaaatcc atgccacttt gtattatcct ataggtaatg aagtttgtga taaaatgtta
     5761 catagttact gattttcaaa cttactgaat ttctgaccga aaaggagtat ctgcatatgc
     5821 ctgaatttca ttcccgtttt gaaaaaattg accgtcgtgt tcccattgat gaacacaact
     5881 gtgccgttca atttgatgtg acgaagtgta agaactgcac cttatgtcgc cgggcttgtg
     5941 ccgacacaca gactgtgttg gactactatt ccctgtccag tacaggcgac atgcccatct
     6001 gcgttcactg cggccagtgt tcgtctgcct gcccctttgg tgccatcgtc gaagtcaacg
     6061 atgtcgacaa ggtcaaggca gccctgaaag accctgaaaa gatcgtcatc ttccagacgg
     6121 ctccggctgt ccgcgtcggc ctgggcgaag ccttcggcat ggaccccggt acctttgtcg
     6181 aagggaagat ggtcgctgcc ctgcgtacgc tcggtgcgga ctatgtcttc gataccgatt
     6241 tcggcgccga cctgaccatc atggaagaag cgacggaact cctgcaccgc ctgcagtccg
     6301 aagaaattcc tatcccgcag ttcaccagct gctgcccggc ctgggtcgaa tttgccgaaa
     6361 ccttctaccc cgacctgctc cagcatctgt cgtcgaccaa gagtcctatc agcatcctca
     6421 gcccggtcat caagacctat ttcgcccagc agaagaacat cgaccccaag aagatcgtca
     6481 atgtctgcgt cacgccgtgc acggccaaga aagcggaaat ccgccgtcct gaactgagcg
     6541 cagccggcct cttctgggat gaaccggaaa tccgcgatac ggatatctgc attacgacgc
     6601 gtgaactggc tcagtggatc caggacgaaa acatcgattt cgccaacctc gaagacggcc
     6661 agttcgacaa ggcctttggc gaagcttccg gcggcggccg tatcttcggc aactccggcg
     6721 gcgtcatgga agcggctatc cgcacggctt accacatgtt cacgggtcgt ccggcaccga
     6781 aggacttcat tccctttgaa ccggtccgcg gcctgcaggg cgtcaagaag gcaacggtca
     6841 tcttcggcca cttcgtcctt cacgtcgctg ccatcagcgg cctgggcaac gcccgcgcct
     6901 tcatcgacga cctcatcaag aacgactcct ttgaagacta ctccttcatc gaagtcatgg
     6961 cctgccccgg tggctgcatc ggcggcggcg gccagccgaa agtcaaactg ccgcaggtca
     7021 agaaagtcca ggaagcccgg acggcatcga tttataagag cgacgaagaa acggacgtca
     7081 aagccagctg gcagaacccg gaaatcgaag aactttacga agccttcctc gacgaaccgc
     7141 tgtcggaaat ggccgaattc accttgcaca cctacttctc cgacaagagc gaccagctgg
     7201 gccgcatgaa gaacctcacg ccccagacca accccatgtc gccgaagtat aaacccccga
     7261 caacagaaga atagcctccc ttgttagggg aggtggccca aagggccgga gaggttgaat
     7321 ttcttagtaa aagcctgcgt agggg
//
LOCUS       DAWABZ010000092         7320 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2730_length_7320_cov_2.916862, whole genome shotgun sequence.
ACCESSION   DAWABZ010000092 DAWABZ010000000
VERSION     DAWABZ010000092.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7320)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7320)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7320
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2730_length_7320_cov_2.916862"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4173..4182
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1890 a         1917 c         1900 g         1603 t
ORIGIN      
        1 ccttttatag cgaacgatgt ttctagttgg cttcattaag cgaaagtggt ataatggatt
       61 tgatttacat cggccagatg ggaggttgta taatcatggc agtagtttcg attgacagtg
      121 atgctgattt ttctgtgaaa gtccttcatc agccgggcat tgtcatcgca gacttctggg
      181 ctccctggtg cgaaccgtgc aagatggttc attcggaaat cagccaggct gccgcgaatt
      241 ttggcgatgc tgtgaaagta gtacgcatca atgttgatac gatgcggaat attgccgaaa
      301 aatatgaaat catggcggtt ccgacattat tgttctttaa gggcggtaag ccactcaaac
      361 gcattatcgg ctatgcctcg caggcggaaa tcagcaagtt cctggcatct gtcgtagcag
      421 aaaacaaggc tgccggtttt gacaacaaaa atattgggat gatataattg aattcagaaa
      481 cgagagggac gggacgacag ttattgtcta ttgtcgttcc tgtatacaat gaacagccga
      541 acattgagaa attctatgaa gaggcgacga aagccgcagc ggcactcgat atggattatg
      601 aaatcatctt tgtcgacgac ggttctaaag atgcaacgcc cctcatcctc agccgcttga
      661 cccagcagga tgagcacgtg cgggctttta tcctggcccg taacttcggc catcagctgg
      721 ccattacctg cggcatggat catgcccttg gcgacgccgt catcaccatg gatggtgact
      781 tgcagcatcc gccgtcgatg attcccgaat tggtccagaa atggcgcgaa ggcaacgacg
      841 tcgtccagac catacgggaa gcgacggacg atgcgggctt cctcaagcgc ataacgtcga
      901 aatggtatta tcgtatactg aatgccgtat ccccggtcca catcacacca ggcgggtccg
      961 acttccgcct catcgataag cgggtcctgg aaacattcaa gcagttccgc gaacacgacc
     1021 gtttcatccg cggcctcatc ggggacatcg gttacacaca gacttttatt catttcgtag
     1081 cacccaagcg ttatgccggg aaatcgaagt tttccgtacg caagatgctt cattttgccc
     1141 tggacgggat catggcctat tccaagattc ccctgcgggt ttccttttat atcggcatgc
     1201 tgagcggttt gttgagcctg ctcctgattt tgcatgtcat ctattgtaat ataatgggcg
     1261 aagccgtgcc gggatggacg acgacgatga tcgtcgactg cctgttcagt gggttccagc
     1321 tcatttttat tggtgtcatc ggagagtata taggacgtat atttgaagag gtgaaacata
     1381 ggcctctgta ttggctgcgg gctgaactcg gccagcataa agacatgtag gaacggtgcg
     1441 ttgtatggcc ttcgagacag agaggagtat ttatgtggaa aaaaatcagt aaagtttgga
     1501 tatttgccgc acttttcgtt tttgccgcac tgccggcgct ggcagcttct ccgctgacca
     1561 ttggcagccg gggcgatgaa gtccgttcga ttcaacagat tcttcgcagt aaaggctacc
     1621 gggtcaatgt caacggcgtt tacaccaatg atacggcgcg ggccgtatcg cagtatcaga
     1681 aagataagaa aatccaggtc agcggtaaag tcggcggctg gacctattat ctcctgacag
     1741 gcaatcgttc catgctgccg cctaagccgg tcatgaagag agggcagggg acgcgcttca
     1801 acaatggcgc tatcagcaaa taccgtcatt tcagcggcgg cgatgctttc ggcaaccagc
     1861 tggtcgattc ggcataccag tatttgggca tcccttacgt attcggcggc aatacgccgg
     1921 ctgaaggctt cgactgctca ggctttacga aatatgtctt ttcccataat ggcatcaatc
     1981 tgccgcgcct ggcagatgaa caatatgaag taggccagcg ggtccgccgc agcgaactca
     2041 tgccgggcga cctggtcttc ttcacgacgt atgaaccggg tgtatcccat acgggcatct
     2101 atgtcggcaa taacaatttc atcagtgcca ccagcagtgg cggcatccgc gtagacagcc
     2161 tcgacagcgg ctattggtcg ccgcgctatg tcggtgctac acgggtccgg agataggcaa
     2221 actggctagg aaacagtcgt cctctagcct ttccttatac aaccgggctg tgagataaaa
     2281 aatcacaaga aaaggttgcc gaacaggggg aaatgagcgt ataattttcc catgtagaaa
     2341 gatatttaga gggagtgttt actatatgaa gtacatgacg ggtaatgaaa tccgcaaagc
     2401 ctatttacag ttctttaaga gcaaggagca cttgatcctg cacagttttc cgctcattcc
     2461 ggaaaacgac cccagcctgc tcctcatcgg cgccggcatg gcaccgctca aaccatattt
     2521 cacgggtaag ctcgtaccgc cgtcgtaccg cgtcacgacg agtcagaagt gcatccgcac
     2581 tgacgatatc gacaacgtag gccgtacggc ccgtcaccat acgttctttg aaatgctggg
     2641 caatttctcc tttggcaact actttaagaa agaagccatt tcctgggcct gggaattcct
     2701 gaccaaagtc ctggaactgg atacggataa attgtatgtc accatctatc ccgatgacaa
     2761 agaagcctat gactactggc ataatatgat tggcctggct gacgaacgga ttttcaaatt
     2821 cgacgataac ttctgggaaa tcggcgaagg cccgtgcggt ccggacagtg aaatcttcta
     2881 tgacctcggc ccggaacgag gctgcggtaa accgacgtgt accgtcggct gcgactgcga
     2941 ccgctacctg gaaatctgga acctcgtctt cacccagtac aaccgcacta aagacggcaa
     3001 gtacgaaccg ctggccaaga agaacatcga tacgggctgc ggtctggaac gactggcttc
     3061 ggtcatccag cagaaagaat cgaactttga aacggacctc atcttcccaa tcatcgaaaa
     3121 agtcatcgcc atttccggcg gcgactacaa tgacccccgt cagaagatgg ccatgaaggt
     3181 cattgctgac catatccgcg ccattacctt catgatttcc gatggcatcc tgccgtccaa
     3241 tgaaggccgc ggctacgtcc tgcgccgtat cctgcgccgt gccgtccgct tcggccgcct
     3301 cctgggcatc aaggacctct tcctggcagg tctcgtcgat acggtattgg acatcctcgg
     3361 cgatgaatat ccggaaacgc gggaacacga agccctcatc aaacagatca tcaatacaga
     3421 agaaaaacag ttcagcgcta ccctggcaca gggcatggaa ctgctcaacg atatgatggc
     3481 ccatgtgggc gacgacaaag tcctcactgg cgctgaagtc ttcaaactct acgatacgta
     3541 tggcttcccc atggaactga ccgaagaaat cgccggcgaa cagggcttca aagtcgataa
     3601 agacggcttc caggaagcca tgaaggaaca gcaggaacgg gcccgcgctg cccataccaa
     3661 agtatcggcc aaagtcgcta cgccggatac gaccaagctg gaccagtcga aactggtcaa
     3721 cgacgaaacg gctgtcaaag cgacgattgc catgctcggc aaggaaggcg tcgaaatcag
     3781 cgaagcccgc gacggccagg aagtgacgat catcctcgac gacaacccct tccatgccga
     3841 aggcggcggt cagctcagcg atacaggcgt cctctgtgct gctgaaggca aggcacaggt
     3901 cactgatgcc caggctctgc ccaatggcct ggtatacctc atcgctgaaa tcactgaagg
     3961 cgtcctccgc acggacgatg ccgtcgatat cgctgtcgac aagcagcgtc atatgaacct
     4021 ggcccgcaac catacggcaa cacacctctt gcaggctgcc ctgcgcaaag tcctgggcaa
     4081 tcacgtcaac caggccggct cactggtcac accggaccac ctgcgtttcg acttcaccca
     4141 tttctcgccg gtcacgcagg atgaactgga tannnnnnnn nnaagccctg gtcaacgaag
     4201 aaatcctcaa ggccgtcccc gtcaccatcg aagaaatggc cctcgatgcg gccaaagaaa
     4261 agggcgctat ggccttgttc ggtgaaaaat acggcgatat cgtccgcgtc gtctccgtcg
     4321 acgacttcag ctgcgaactc tgcggcggct ctcatgtggc caacacgtcg gtcatcggta
     4381 cgttccgcat taccagcgaa accgggactg gctccggcgt ccgccgtatc gaagccgtta
     4441 ccggtccggc tgccctcaag ctggctaagg atgaatcgca ggaactgcag cagctggcag
     4501 gcctcttgaa aggcaaggtc agcgatgcgc cggccaaact ggaagctctc ctggcccagc
     4561 tcaaggatac ggaaaaagaa ttggctcagc tcaagaaaga cgcagccctg tccgatatgg
     4621 acagcatcct ggcagctaaa gaagatatcg gcggcgttcc tgtcgtcgca gccgtcgctc
     4681 aggctgattc tatggataac ctgcgcgacc tggctgatac ggtcctcgac aagctcggca
     4741 gcggcgtcgt cctcttgggg atgaccaatg gcgataaagt caacttcgtc tgcaaagtcg
     4801 ccaagagcga taccaagaaa ggcctccatg ccggcaagat catcaaagct gctgctcagg
     4861 cagctggcgg caatggcggc ggccgtcccg atatggccca ggcgggcggc aaggaaccgg
     4921 aaaaattggg cgatgccctt aaagccggtg ccgcagccgt acgcgatatg ttagcataaa
     4981 tttttggcag tcatgccagt tgaataaaaa ggatgtgagt gaacatggca gtaagcgacg
     5041 aaacgatgat tatgcaaaac cgaagtgaag aacagaccgt tgcatccatg attctggaag
     5101 atgtatatca cgcattggaa gaaaaaggat acaacccgat taatcagatc gtaggctatc
     5161 tcctgtccgg tgacccgacg tatatcacga gccacaacaa tgctcgcagc aatatccggc
     5221 gcatcgaacg agatgaactc atcgaagaat tagtccgttc gtacgtcaaa cagcacaact
     5281 tataggagac atatgagaat tttaggactc gatgtcggtt ccaagaccat cggtgtcgcc
     5341 tgcagcgatg ccttgctgat tacggcccag ggcgtagaaa cgatccgccg taaatccgat
     5401 gaagcagact ttgcccgtct gcgcgaactg atcaaagaaa aagaagtaca ccgcatcgtc
     5461 gtaggcaagc cgcgtcatat gaacggtgac tacagcgata atatgaaaaa aatcgaagaa
     5521 ttcgtcgaac gattccagca aaccgtcccg gatatagaca ttgtctattg ggatgaacgc
     5581 ctgacgaccg ttatggccca gaatgtatta aaggaaggca atgtgcggcg cgaaaagcgc
     5641 aagcagtttg tcgataaaat ggcagcaatc cttattttac agaattattt agacgctcat
     5701 gctcattaag gaggactact atgtcggaag aaatgaacaa cgaattggaa gaagtcatca
     5761 tcgtgaccgc tacggatgac caaggcaacg aagtggaata tcaggaagta cagcgcctcg
     5821 acctcgatgg taaagtattc accctgctcg tcgaagtcac ggaagacgaa gaaggcggcg
     5881 atgccatcat cgcccgcgtc gaccaggaag acggcgaaga cgtctacgtc gcaccgacgg
     5941 acgaagagtt cgacgcagcc aatgcaaaat tcgatgaact cttagataag taattgaagg
     6001 ggccctgcat gaaggtttcg gccatcatgc aaggcctttt tgctttatcg ttcatcgttc
     6061 gtcgttcatc gtgatgggat gctttttttc gacctacgag gaacgaccta cgaggaacga
     6121 aaaaaggagg tcccatggat ttaccgtcga tacagcagct gcggaatttc gtcatttacg
     6181 ggaagtatgg aaatttcacg acggctgcca atgccgccaa cattacccag tcggctttca
     6241 gtgcgcagat gaagaaactg gaagacctcg tcggcgtccg cctcatcgag cgctcgaaca
     6301 ggggaagtca cttgacgccg gctggcaaga tgttctacac gaaaatagcc cgcattttag
     6361 gtgaattaga agaagccatg agcgaactgc cagggcggac agggaagacc cagcccctgt
     6421 cggtgggaat catgctgtcc ttaggcgacg tccacatgaa ccgccacctc gcttatttcc
     6481 aggagcacca cagcggcgct tctttccggg tctacaacct ggaagtccgg gagatgatcc
     6541 aatggctgaa agacgataaa ctggatatcg tgtccctctt ttatctgccg gcactgcacc
     6601 tggaagacta cgagaaggtc ttttttggca tcgagaacct cgtctattac gggccgaacc
     6661 tggatctgcc ggaacatgcc gtgacgcctc aatacgtggc atcccagccg ttggcgcaat
     6721 attcgccgca atacgaaatg aataactaca tcaatcacta tttcacggcc catgcctgcc
     6781 cgcctcagga aacgcaggcc tggttctcta cgccctatgc catgatgaac tattgccagc
     6841 agaaccgcat cggagctatc ctgccggaac gattcctcca ggccatgggc atcacccagg
     6901 gctactgtgc attggaaccg ccgctgcgct tgccctgttc tctggtctat aagaaggaca
     6961 atcccaagta cgcgacgata caggtcttcg tcgactatat ccgtcgcgtg tatcatatca
     7021 aaaaataatg ataaaggtat ctgccatcgt aaaaaacgat gacaggtatc ttttttttgc
     7081 gttatacaag ataggagaga aggtgtagaa tctagcttgc aaatacgaaa ggagcagtga
     7141 atacagaatg tcgaatcaat cactttggaa caaagacttc cttctcgaca caggtatcaa
     7201 ttttgtagtc tatctcattt attatttatt gatggttatt atcgccgtcg tcgccaaaga
     7261 ccagcttcat gcttctctcg gtgaagccgg cctggcttcg ggcatcttca tcgtcggcac
//
LOCUS       DAWABZ010000093         7188 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2794_length_7188_cov_3.760550, whole genome shotgun sequence.
ACCESSION   DAWABZ010000093 DAWABZ010000000
VERSION     DAWABZ010000093.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7188)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7188)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7188
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2794_length_7188_cov_3.760550"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1455 a         1905 c         2006 g         1822 t
ORIGIN      
        1 cgagacagta aaaactgtct cgttaccaag gtctaacttt ttggggtcac atcagaaacc
       61 ttcgtgcgac agcccctctt ttaagtatca agtttgtagt ttttagtttg cagttattgg
      121 ctaaaaattt aacctctttc tacaaactcg gcactacaaa ctttaaacta tcttttagcc
      181 ttcgatttcg gatacgacac cggcacctac ggtacggccg ccttcacgga tagcgaagcg
      241 caggcctgct tcgatagcaa tcggtgtgat gagttcgact tccatcttga cgttatcgcc
      301 aggcatgcac atttctgtgc cttccggcag ggtgatgaca cccgttacgt cagtcgtgcg
      361 gaagtagaac tgcggacgat agccgttgaa gaacggcgta tgacggccgc cttcatcttt
      421 ggtcaggaca tagacctgag ccttgaattt cgtgtgcgga tgaatcgtgc ccggtttagc
      481 caggacctgg ccgcgttcga cgtctttgcg gtcgatgcca cggagcagtg cgccgatgtt
      541 gtcgcctgct tctgcgaggt ccaggatctt acggaacatt tcaacgcccg taacgacggt
      601 atctttcggt ttatcagcga ggccgacgat ttcgacagcg tcaccgactt tgatcgtacc
      661 acgttcgaca cggcccgtag caaccgtacc acggccggtg atggtgaata cgtcttcgac
      721 aggcatcagg aacggctggt ccgtcggacg tttcggcgtc gggatgtagc tgtcgacagc
      781 atccatcaag tcgaggatgg atttttcagc ttccggatcg ccttcgaggg ctttcaatgc
      841 agaacctacg atgataggta cttcatcgcc aggataatca tattcagtca ggaggtcgcg
      901 gacttccatt tcaacgagtt caatcagttc cggatcgtcg acctggtcag ctttgttgag
      961 gtatacgacg atagccggta caccgacctg gcgggccagg aggatgtgtt cacgagtctg
     1021 cggcatcggg ccgtcagctg cggatacaac gaggatagca ccatccatct gagctgcacc
     1081 agtgatcatg ttcttgacat agtcagcatg ccccgggcag tcaacatgtg catagtggcg
     1141 tttttccgtt tcatattcga cgtgcgccgt gttgattgta ataccacgtt cacgttcttc
     1201 cggagccttg tcaatatcgg agtaatcttc gaacttggca aagcccttct ttgctaatac
     1261 tttcgtaata gcagccgtta atgtcgtttt gccatggtcg acgtgaccaa tcgtaccgat
     1321 attaacatgc ggttttgttc tttcataatg ttcttttgcc attgtaatca tttcctcctt
     1381 taggaaaaaa acagctaccc acttgtattc tatcacacat ccatactctt tgcttaaaaa
     1441 aataggaaaa tttaaacaaa aaatattttt gcatatagaa ataacctcgt atgcacgagg
     1501 ttattagtgt ggtggcggct cggagattcg aactctggac actgcgggta tgaaccgcat
     1561 gctctagcca actgagctaa gccgccatgt gtggagctat tgaccgggat tgaaccggtg
     1621 accttatcct taccaaggat acgctctacc gactgagcta caacagctta tgaactggtc
     1681 aaagccaatt atgaagttgt ttttttacct gacagtaaaa aaatttggta gcgggggcag
     1741 gacttgaacc tacgaccttc gggttatgag cccgacgagc ttcgaaggca aacgccggca
     1801 gatttacagt ctgctccctt tagccactcg ggaaccccac catttactgg agccaacgat
     1861 aggacttgaa cctacaacct actgattaca agtcagttgc tctaccaatt gagctacgtt
     1921 ggcttactgt tccgtggaac gtagtctatt atatcaaagg actgacgttc tgtcaagaac
     1981 ttttttatca gcttttctta tttcttctga cgaggcagaa ccccgtttca aaaggctctg
     2041 tcagaaccgc tgacttgatt agtatactat accccacgga ataatgcaag tgctttttac
     2101 acttttttta aattaatttt atctaccctt catgctgcgt gtccaggttg aggaacagcg
     2161 tgaactcgtt cttgaagtag agcttgaccg acccgacagg gccgttgcgg tgtttggcca
     2221 ggatgacttc tgtaatgtgc tggttctccg tttccttatc gtagtaatct tcgcggtaca
     2281 agaaggaaac gatatccgca tcctgttcca gggcccccga ttcgcggagg tcactgagca
     2341 taggccgttt atcctgccgg ctttcgacac tgcggctgag ctgggacaag gcgatgagcg
     2401 gtactttcag ttcccgcgcc agggccttga gggaccggga aatctcggag atttcctgct
     2461 ggcggctttc ggcattgcgc ccctgcatga gctgcaggta gtcgacgata atcaggtcca
     2521 ggccgtgttc cgacttgagg cgccgcgctt tcgatcgcat ttcggcgaca gagatgcccg
     2581 gcgtatcgtc gatgtaaatg ggcgactcat aaagcttgtc gcaggcatcg gtcaggcgcg
     2641 tccactcttc atcgctgttg agctggcctg tgcgcagttt ctgcgagtcg atatgggcga
     2701 tttggcagag cagtcgctgg actagctgtt cctgggacat ttccagggaa aagaaggcta
     2761 ccgtcttatg ctgacggacg ccgacgttct gggcgatgtt cagggtaaag gccgtcttgc
     2821 ccatactcgg acgggcagcg accaggatca ggtccgacgg ctgcaggcct gacgtcatgc
     2881 ggtccaggtc gcggaatccc gtcggcaggc cggtgaggcc cgctttattt tcatagagct
     2941 tggtgatctt gtccagggtc acttcgacga cggcgccgac ggaagcgaaa tcgccgcggt
     3001 tctttgtatc cgacagctgg agaatgcgcc gttcggcatc gtccaggagg gcttccgttt
     3061 ccttttggcc gtcataggct tccgtcgtca gttcggtaca cgtcgaaatg aggttgcgcg
     3121 ccagggcctt ctcggcgacg atattggcgt aatattcgat attggccgct gacccgacca
     3181 ggttcggcag gttcaggacg tattcgagac cgcctacatc gtcgagcttc ttcatgcgtt
     3241 tcaactcgtc cggcagggtg atgacgtcga tttctttatt ttcccgatgg aggtgttcca
     3301 tggcctcgaa gatgatgcga tggacgtcgc ggtaaaaatc ctgggactgg agcttttcca
     3361 tagcgacgat gacggcatca tgggataaga gcatcgcccc caggacggct tgttccgcct
     3421 ctacgttctg cggtggaatc cgctgttcca tattaggctc cctcctgttc gaccaacatc
     3481 agtttcagta cgtcgtcgat ggtgacggca tagtggatat tcaggcctaa atggcgttcc
     3541 gggatatcat gagcattttc tttcgggatg atgattcctt tcatgccagc ctgacgggcg
     3601 ccataagcct tttcaaagac gccgccgacg ggcttgacct ggccctggat ggaaatttcc
     3661 ccggtcaggg ccatgtcctg gcggaccggc ttgcctgtga tggccgagat gagggccgtc
     3721 gtaatggcac agcctgccga cgggccgtcg atattgccgc cgccgacgaa gttgatgttg
     3781 acgtcgtaat cggacagttc cttaccggtc atgcggcgga cgacagcggc tgcgttgaac
     3841 atggagtcct tggccatgga accggccgta tcgttgaagc ggtaataccc cttccccggc
     3901 gcccgcgccg gaaaggcgac ggcttcgatt tcaatggccg agccgaggta accggcgacg
     3961 ccgaggccga agaccttgcc gacgacaggc gtatcgctgg ccttttccgt cacataaggc
     4021 acgaggcggc tgatctgggc gacgcggcgg acgtggtcgc aggtgatgcg cacgtggtcc
     4081 ttggagcccg atttaaagac agacaggcca tatgtatcgg ccaggatgtt gacggccttg
     4141 cggccttcga tggtgaactc actgatcaat ttggccacgc cgtcttccag ttccgccccc
     4201 agctgggcag cggcattggt gacaatctgt tcgatatccg acgggatgag gggttcaaag
     4261 aagatttcag cgcagcgcga ccggatggcc ggattgattt ccgacgggtc ccgcgtcgtc
     4321 gcaccgatga ggatgaagtc ggccggagcc ccttcggcaa agagtttctt gatatacttc
     4381 ggcacattcg ggtcgtcttc gtcgtaataa gccgattcaa aacggacccg cttgtcttcc
     4441 atgactttca gcagcttgtt gagcagcatc gggtccactt ccccgatttc gtcgatgaag
     4501 aggatgccgc catgggcttc ggtgaccagg cccggcttcg gttccgggat ccccgtatcg
     4561 gccaggtcgc ggcgggcgcc ctgataaatc gggtcgtgga ccgaaccgat gagggggttg
     4621 gtcatatccc gcgaatccca gcgcagtgtc gtcccgtccg tttcgataaa gggcgcatca
     4681 gggccaaacg gtgtaaaggg cagcttcttg gcttcgtcga ggacgatgcg ggcagccgtc
     4741 gtcttgccga cgccaggcgg tccgtagagg agcaggtgct gcggatagac agacgataac
     4801 ttgctctgca tggcttccac ggcccgttcc tggccgacaa tagcccggag ctgtttcggc
     4861 cgtacccgtt ccatgaccga ttccgtaaga tggacgtttt ccaggtcgtc caattccttg
     4921 agcttgcgct tggtctgcgg cgtttcgacg tcgtgcagtt cttccttgat gagttccatc
     4981 ttgatttccc gcacgtattc ctgctgtttt tcatccatgc gctggctgat tttcttttcc
     5041 agccgttctt ctacggcctg acgggccagc atctcagcca ggaccatttc cagttcattg
     5101 aggacgttga tgacttcatc gtcatcgggt actttatcat acgagacgtc ttcatagatg
     5161 aggcgctgca gtccgaccag gcgctggcgc gggtcttcgg aatggatgta cgacaaggca
     5221 gagtacttac tggcccggag gaccatcttt tcagacccta taagaccagt aaaagcgcca
     5281 taaagcacgt taacctgacg gcggagttct tccgccgtcg gttcgaggta cttatggcgc
     5341 tgtaataatt tgtccaataa ttctttcatt gtatcccctt atggcttatg atcagccttc
     5401 tacaacgtgt attttgatga cactggtaat cgtcggatgg acgcggatgg taacatcgta
     5461 atcgccgagc gatttaatcg gatccttgat ttctactttt ttcttgtcca ggtcgatatc
     5521 gtactgggcc ttggccgctt cgctgatagt cttgcccgta atggcaccga agactttgcc
     5581 gccttcgccg actttaacgg gaatggtcag ttcgactttc ttcagctggc tggccaggat
     5641 gacggcttca tccgaagcga cctgggcctt atgcttggca gcggcttttt tctgtttggc
     5701 atcgttgagg ttttccgatg tgccgggagc ggctaattta cggggcagga ggaagttgcg
     5761 gccatagcct tccgatactt cgatgatgtc ccctttctta ccgagtttct ttacatcctg
     5821 taataagatt actttcattt ttcatcaatc tccttgtctt tatctatgat ttcatggagg
     5881 gctgccatga cgtccccctg ggcttcgacc agggtccggc cacgcagctg ggcaccggca
     5941 acggtacggt ggccgccgcc gccgagagct tccatgacta attgtacatt gatttcgcct
     6001 ttcgagcggg cgctgacacc gatgcagtca tcggccagat aatagaaagt gaagctggct
     6061 tcgacgctgt cgatgttgat gagcatatcc gctacctggg cggcgatgat ggtggcattg
     6121 cgctgaccct tggggcagct ggccatggcg acgccgtcgc tgatttgggc accggacaag
     6181 atgagggccc ggtccttgac ggtatcgaag tcagagctga acagttcgcg gacgatatcc
     6241 ggatccgctc cgcagcggcg cagatacgac gcggcgtcga aagtgcgcac gccggtctgg
     6301 acggcgaagt tcttggtgtc gacgatgatg ccggcataga ggcccgtcgc ttcgatcttc
     6361 gtcaattcta catcttcctg gaaatattgc aagagctccg tcaccagttc gctcgtcgaa
     6421 gaactcgacg tttccgtata ggtcagcaag ggcttcttga tgaagtccga actgcggcgg
     6481 tgatggtcga tgacgacgcg ccggtccgtt tcgtccagca aagccggggc tacggtcatg
     6541 tccgggcggt gcgtatcgac gatgaacagc agggtctggc tgttgcagat gttctccgcc
     6601 gtttctgccg aaatgagcag gtccttgaaa tcgggattat ccatgatctg atcttccagg
     6661 cgccggatgg cgtcggtctg attgctgacg acgacatgta ccggcttgcc tgccaggcgg
     6721 gccatgtggg ctacgccgac ggcagcgccg atgctgtcat agtcttcccg ttcatgtccc
     6781 atgaccagga ccatatcgct cgtatcgatg agttcgtgga tggcctgggc gacgacgcgg
     6841 gcccggacgc gggtattctt ttcgacgcag cgcgtcttgc cgccgtagaa gtgaggcgat
     6901 ccgtcctctt catagacgac gacctggtcg ccgccgcggc ccagagccag gtcgaggccg
     6961 gcccgggctt tcgtagccag ttcgttgaaa ttggctttgc ccgccgcaat gacgtcgctc
     7021 gggaagaggg cgatgcccat gctgagcgtc acggggatgc ggttgaccgt atggatggaa
     7081 cggatttttt ccaggaaaga aaagttatct tccttataat attccagcgt atcccggctc
     7141 atgcaggcaa tgtacttctc attgccgtaa gcccagataa agcaatta
//
LOCUS       DAWABZ010000094         7149 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2818_length_7149_cov_3.251903, whole genome shotgun sequence.
ACCESSION   DAWABZ010000094 DAWABZ010000000
VERSION     DAWABZ010000094.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7149)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7149)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7149
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2818_length_7149_cov_3.251903"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1460 a         1892 c         2040 g         1757 t
ORIGIN      
        1 agcggcgaga ttcgaactca cgacctcttc caccccaagg aagcgcgcta ccaagctgcg
       61 ccacgtcccg tcgacttttc tattttaact gattcgacag gacttgtcaa tagtctattt
      121 gttatctctt ttacggaaaa acggcagggc tacttctggg aacagggtat agctcaaaac
      181 gtcttccggg ctggcatcgg ggaatccttc gtcagccagg gctttcttcg atttttccag
      241 ttccggttcc aagtcgtcgg ccgggcggtg ggtgatgacc ggttcgtcgc cgatgcacat
      301 tttgcggaac ttttcatcga tcttgccggg gacgcggccg tatttcccgc ggacgatgtc
      361 gcggacttcg ttggtgacga tcttatagcg gccatagagg acgttgaggg ctgctgccga
      421 gccggtgatc tggctggtcg gcgtaaccag cggcggatag ccgaggtcgc ggcggacgtt
      481 gggcatttcg gccaggactt cgtcgaagcg gtcttcggcg ccctgggctt ttagctggtt
      541 atagaggttg gtcagcatgc cgccaggaat ctgatatttg cggacagccg gattgatctg
      601 gctgggcatg gtcaatttga attcgtcttc cagttctttg cggacatgct tgaagtggtc
      661 gacgatgggc cacaggcggt cgacgtcgat gtccgtatca tagatgctgt gctgcagagc
      721 catgaccatc gattccgtcg ccggctggct ggtgatttcg ccgaatggcg acaaggccgt
      781 gtcgatgatg tcgacaccgg cttcgatggc cttcatatag gtcatttcac cgaggccgca
      841 cgtacagtgc gtgtgcagtt caataggcac ggacaagtgc ttcttgagca tgcggatcag
      901 gttgtatgcg tcatacggcg acagcaggcc ggacatatcc ttgatgcaga tggagtcgac
      961 gcccatgtcc tgcagttcct tggcgacctt ggtgaacgat tccatcgtat ggatagggct
     1021 gatggtatag accagggcgc cctggacgtg gacggacttc gtgtctttcg ccgccttgat
     1081 ggacgattcc aggttgcgcg ggtcgttgag ggcgtcgaag acgcggatga tgtcgatacc
     1141 gttatcgacg gcgcggttga cgaattcgta gacgacgtcg tcggcataat ggcggtagcc
     1201 taagatattc tggccacgga acaacatctg caagggcgtc ttcttgacgt gcttcttgat
     1261 ggtccgcagg cgttcccacg ggtcttcgtc gaggaagcgc atacacgtat caaaggtagc
     1321 gccaccccaa cattccaggg cattatagcc gacttcatcc atcgcttcca ggaccggcag
     1381 catctggcgc aggcgcatac gcgtggccgc aatggactgg tggccgtcgc gaaggaccgt
     1441 ttctgtgatt tttactcttg ccattctatt tcctccaatg ttgtatttat cttcaaaggt
     1501 tggatgcgaa aatcatacgt actattttat tatatcatag taggcccggt tttgctattc
     1561 acatcgaggt catacacagt ccggccctgg cagacacgga tatgaccaag gggtacctca
     1621 tcctgatgcc aggtcaggtg ctgaggcagg ctccagcagg ccgtcttgac ggtaacgttg
     1681 cgatagcccc ggacgcggga cgtatcgcgc cggttatagc cgatggtgat agccggttca
     1741 tcgggcaggc tgcggaggaa ggacaggagc tgccgaccat agcggcgagt atcgactaat
     1801 tcgcccatag ccggcgtgta ggccccggcc aggacctgcg tgtcgtcatc caggttttcc
     1861 gtggcttgca ggcccgtccg gatgcaattt atgccctgtt ccaggaagtg ctgcttcatg
     1921 taggccgccc ggacgacgcc ttcatggatg gtcaagggct ggtagcggcc ggcccgccag
     1981 ctggcagcca ggtccgtccc ggcgaggacg gcaacgggat agatacgggc caggtccggc
     2041 tggagccggc agatagccgc tgtcgtccgg accaggctcg gccagtcttc gccgggcagg
     2101 ccgggcatga gctgatggcc gacggtgaag ccgtactgcc gtaagagggc tgtcgccgcc
     2161 acgacatccc ctgccgtatg gccccgccgg gcctgctcga gaacggtatc gtccatggac
     2221 tggacgccca gttcgacgca ggtcatgccg tactgctgca ggcgctgcaa tatctccggc
     2281 gtaatgcagt ccggccgggt cgaacagcgg atggcgtcaa tcgtaccggc ctccaaggct
     2341 tcgtaagccg gctgcagaag gccttcctgt acctccccgg gcatggccgt gaaactgccg
     2401 ccgtagaaag ctgcttccca gtagcgcggc gtccgggccg acgccgtata ggacgcaatg
     2461 agacggcgca cctcatcggg cgacggcaag ccctggtgtc ccgtaatctg ccactgattg
     2521 cagaagacac agcggtgggg acagccgata tggggaatga aaatagggat gatagacgtt
     2581 tgcatgatgc ctcctgtgaa aaaaagaggc tgtctcatcg acagcctctt tttaagtttg
     2641 aagtttgtag tttttagttt gcagtgaacg gttaaaaatt taaaaccatc cgctacaaac
     2701 tgcagactcc gaactacaaa ctataaagaa aaaccttcgc ctttcaaatc catgagcgct
     2761 gtatgggctg catgttgttc ggcgtctttt tttgtttttc ccgatccttc gcccaggact
     2821 ttgccgttga ctttgacttc tatgtagaag gtcttgtcgt ggtccggtcc ttcgctgcgg
     2881 cacagttcgt attcgatgtg ctgcgggccc ttttgctgga cgtattcctg gaagagggtc
     2941 ttatagtctt tttcataatg gccgctcttg accaggtcca agtagccctt gagctggtga
     3001 aggatgaatt tacgggcttc ttcataggaa ctgtcaatat aaatcgcacc gacgacggct
     3061 tcaaaggcgt cggccatgat gctcggccgc gtgcgtcctc ccgtagccag ttcccccttg
     3121 cccaggagga tatagtcccc catgtggaac ttggcacaga ccgaagccag cggcgtttcg
     3181 ctgacgacac tggcccgggc cttggacagt tccccttcgg gcatgttggg ataatgaagg
     3241 aatagatatt cacctacgac caggtccagg atggcatcgc ccaaaaattc caggcgttcg
     3301 ttgtaatagc cgtggctggc cttgttttca ttggcatagg atgaatgggt cagggcctga
     3361 tccaaaatga ggagattgtg gaattccttg aggcccaaga cctgtatgaa ttgttctaac
     3421 tgttgttttc gttcggtatc catcaggtca gtcttcttta ttattcagcg tacttcttga
     3481 agagcagggt tgcattatgg ccgccgaaac cgaaggagtt ggaaatggcg acgttgacgt
     3541 cagcttctac ggctttattg ggtacataat tgaggcccag ttctttcaat tcatcttccg
     3601 gttcgacgag gttgatcgtc ggatggacct tgcccgtttc gatggtcatg gcgcaggcaa
     3661 tggcttcgac agcgccggca gcgccgagga gatggccggt catggatttc gtcgagctga
     3721 tgagcatgtc tttggcatgg tcgccgaaga cgcgggcaat ggccttcgat tcgttgaggt
     3781 cgttgaggtg cgtcgacgta ccgtgagcat tgacgtaatc gacgtcttcc ggctggaggc
     3841 cggcatcttt gacggccaga gccatgcatt tggactgctg gatgccttcc ggagccggag
     3901 ccgtgatgtg ataagcgtcg gcattgctgc cataaccgcc gatttcacag taaatgtgag
     3961 cgccgcgttt cttagcgtgt tccagttctt ccaggaagac gacgccggcg ccttcgccca
     4021 tgacgaaacc atcgcggtct ttatcgaagg ggcgcgatgc atgagccggg tcatcgttgc
     4081 gcgtgctcaa agctttcata gcggcaaagc cagctacggc accgggagat acagcggctt
     4141 cggtaccacc ggcaacagcg gcgtcgagtt cgccgcgctg gatcatgcgg taagcagcgc
     4201 cgatggagtt cgtgcctgtg gcacaagccg tgacgacgct ggcacaatgg ccctggaggc
     4261 cgaagaagat ggatacctgg cccgatgcca tgttggcaat catcttcggt acgacgaagg
     4321 ggttgacgcg agacggacct ttcgcgaaca agcctttgta caagttgtgg agcgtttcca
     4381 taccgccaat gcccgtaccg atgatggtgc cgatgcggtc gcggtcttct tcatccagat
     4441 tgattttgga atcgtcaaga gccagtttgg cagcagctac ggagaactgc gtggacgggt
     4501 ccatatggcg gacttcttta cggtcgatgc cgtagtcagc ggggttaaaa tctttgactt
     4561 cgccggcaat gcgggcagca tattcggttg cgtcaaagtg ggtaatgggg ccaatgccgt
     4621 tttcgccgtt gagcagggca ttccagaaag catctttacc gatgccaaca ggagaaatga
     4681 cacctaaccc tgtaattact acgcgttttt ccaaagtttt cacctcatta taaaaagtaa
     4741 cgtactatca gtcgggaatg gcttccattt cccgtttgat gttggcaaat atatccttga
     4801 ccggcagaat gtcgtggatt tccatcatgc gcctgccgga aaataccaga cctgtttcga
     4861 catcgccctg ctgagcccgc gtcaatgccc ggatgatgca gtatttatga ctgcacttct
     4921 tgagacactg gtcacatgtt tccggcttcg gtgccgtccc caggaggatc cgttcggaga
     4981 aaggcgtgcg gatagcctgg ccgggaaggc cgacaggact gtgtatgagg atgaagtctt
     5041 ccggtttatt ggcccggaga tagactttct tcaattcttc agaaccattc gattcaacgc
     5101 tggcagcaaa gcggctgccc atctggacgc cattggcgcc gaggcggagc atttccgcaa
     5161 tgtcttcacc atgcagtacc ccgccggcac cgataacagg gatgtcaacg gcagcgcgga
     5221 cttccggcac gatgaggcgg ctcgaacggt tggtccccaa atgcccgcct gcttcagctc
     5281 cttcgacgac gacggccgaa gcacccaggg attgggatat tttagctagt ttggcagaag
     5341 acacaatagg gacaataggc gtgcccgatg ctttgcccca ggcgaacatg tcccgggaaa
     5401 agcctgcgcc tgccacaacc agatcgattc cttcttcaat agcagtggtc acgatgccct
     5461 tgaattcacg ggctgcaacc attacgttta tacctatgat tccatgagga gacaattttc
     5521 tggctaactt aatttcatgt cgcaattcgt caaaaggcat gccagatgcc gcaataaggc
     5581 cgataccgcc ttcattggcc acggctgcag ccagccgggc tgtggacaaa cggatggcca
     5641 tgccgccctg aatgattggc acctttgcta cgagcttacc tattcttaat tctggcagct
     5701 tcactctgtt ttcctccatt caagataccg ggaggggaag acatgcaaca tgcctccccc
     5761 ttcgggctct tattgcgtac ttacgctttc ttttcctttt caatgtaatc aactgtatcc
     5821 ttgacagttt tgattttttc agccacatca tcagggattt cgatattgaa ttcttcttcg
     5881 aaagccatga taagttcaac aatgtccaag gaatcagcgc ctaaatcatc gatgtaagca
     5941 gcgtccattt tgacttcttt ttcgtcaacg cctaactgtt caacaacaat gctcttaact
     6001 ttttcaaaag tatcctgttc gctcatgtcc attcaccccc tttcagtgga gtatgctttt
     6061 tgtattacat taccatgccg ccgtctacgt tgagggtctg gccggtaata tagctggcat
     6121 cgtccgatgc caggaacaag acggccttgg ccacttcttc cggctgggcg acgcggccca
     6181 tgggaatggt cttgaccatg gcatcgacgg cgtcttcacc gaggacagcc gtcatatccg
     6241 tggcgatgca tcccggtgct acggcattgc agcggatatt gcgcgaagcc agttctttgg
     6301 ctacggactt ggtgaagcca atgaggccgg ctttggcagc ggcgtaattg gcctggccgg
     6361 cattgcccgt ttcgccaacg acagacgtaa tgttgatgat cgaaccactg cgctgtttca
     6421 tcatcagccg cgatacggcc ttggtgcagt ggaacgcccc attgaggttg gtattgagga
     6481 cggcctgcca gtcttcttct ttcatgatca tgagcagtcc gtcgcgggtg atgcccgcat
     6541 tattgactaa gatatcgacg cggcccagtt ctttcttgac ggccttcacc atgtcggcta
     6601 cagctttttc atcagagaca tcgcactgga tgaccatagc ctgagcgcct ttttcttcga
     6661 tggcctgctt cgtttcttca gcagctttcg tattgccagc ataaatgatg gcgacgctgg
     6721 caccggcttc agccagggcg atggctacgg cgcggccgat gccgcgggag ccgccggtga
     6781 cgatagcagt tttccctgtt aagtgcattt tatcgaacct cctggaaaaa ttcaagcgtt
     6841 ttctgtaagg aaggaatatc ttctacattt tcgctgtgga ttttgcggtc gattttacgg
     6901 ttaaaaccgc aaagggtctt gcccgggccg acttcgacga aggttttggc accgaaagcc
     6961 tgcatggtct tggtacagtc gatccagagg accgggctgg cagcctgttt gaccaggttg
     7021 gctttgatat cggcagccga tgtttcgatc tggccgttga cattggctac gacggggatc
     7081 ttggcatcgg aaatggtgat gtcagcgagc acttcggcca accgtttggc agccggttcc
     7141 atgagcgtg
//
LOCUS       DAWABZ010000095         7029 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2873_length_7029_cov_3.777172, whole genome shotgun sequence.
ACCESSION   DAWABZ010000095 DAWABZ010000000
VERSION     DAWABZ010000095.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7029)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7029)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7029
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2873_length_7029_cov_3.777172"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3056..3065
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1672 a         1857 c         1878 g         1612 t
ORIGIN      
        1 ccgtatcggt aatgtcttca aaatagagga tgaggtaatt gtcatcgtca gcggcttcgg
       61 tctgcagata cttgtagacg acgcggtaat agcgttcgcc gatgtgttca aagaaatatc
      121 ccgatttccc ccagaactta tcgactttca agttcggcat gatgtggtcc agctgctggt
      181 cgttgtcgat gtcgccgacc cagtctcgga agacgctgtt ggcccagcac aagttggact
      241 gcatgtcgat gatggcaatg ccgataggca gattctgcac ggcatacgtc gaagcctggt
      301 cgacgctctt ggagatggca tcgaagaact ggctgatttc ccggttgcgt tcgttatggt
      361 tcttgcgggt caggacataa gccccgccga tgaggatcag ggccagcaag gcgatgaccc
      421 aattatatac ggcaatgatg ataagcagca agacggtaat caggaagaaa ggttcttccg
      481 accgccttga cagcaacgtt ttaaacatgg ttccgccccc tgcgtactaa agcgtacgac
      541 ctttttttct ataattcgtg agcatgtcga agatgccggc taaaaaggca gcatacgaaa
      601 aaacaggcat gactaaaaat gagatgagga tcagggcctg aaggccattc cctaaacgga
      661 aacggctgct gaggagataa aagacgaagg caattccctg gatacagatg aagaagaagg
      721 caatctgatt gagattcatg ccgatgatat tgagccaggt aatatcccgt gtcactcccc
      781 agtatttcat gaccaatgcc aatatatata agtatatcat actacggggt atttcccaat
      841 aacgaatagg caaaaacgcc ttgagcgaaa agccgagacg gcgcaggatg acctgggaaa
      901 tcttgatgtt cgcgtaggcg atgatggccg tccccagaca gaggaacatg ggcagcagcg
      961 acggcagcac ctcccggacc tggttgagct gggcccgcgt ttctgccaat ttgacgtcag
     1021 acatgccctg ctgctggtac tgggccatca tgctgtccat gaactgcgtg aagaaatcgt
     1081 tcatctgggt aaagacgttg acgtcgagga caacatagga caatagggcg atgacgacga
     1141 agacggcgac cagggccgct gtaaccgtcc ccaagaggcg tacagccgac cattcatagc
     1201 gaaagccagc tcccagggcc aggccgacgg caccgaagct caggaattgg accaatgccg
     1261 tcaccgggtc gatgaacaac cccatgagga agcctacggt cacgcccagc agcaaggtct
     1321 ggcgcagacc gtatttcatg aaaatgatga ccatgggaat gggcatgatg aaataaccaa
     1381 taaaagaaaa aatcggcacg tatgtactga gaagcgcaaa gaccagcata atggcggcaa
     1441 aacagccggc cgtcgtcagc ggggtgacac gcctctgctc cataacagtt ccccctttcg
     1501 tcgggactga gaaacagcgg aggcacgaga tgcctccgct gtcacagtaa caaatatcaa
     1561 attatacttc catgatgatc ggcaggatca tcggacgacg ccgtgtcttt tcatagagat
     1621 aacggctcag cgtatcgcgg acctgggact tgatgacagc ccattcgcgg atattaccgg
     1681 cttcgcagcg ttcgaggacg gcggcgacgc ggtcatgagc ttcgcggagc aattcttccg
     1741 aatcgcggac atagacgaag ccgcgggaaa cgatatccgg gccggccagg ggatggctcg
     1801 tccctttggc cagggtcatg acgacgatga cgacaccttc catagccagc tgctggcggt
     1861 cgcggatgac gatgttgccg acatcgccga cgccgagacc gtcgatgaag acgcggcctg
     1921 cattgacatg gcctgtctta tggccggaac ggttcgagaa ttcaaagatc tggccgttgt
     1981 cgccgatgag gacgtggtcc ttggcaacgc ccatcatgac ggccaggtcg ccgtgcgtct
     2041 tgagcatgcg gtattcgccg tggacaggga tgaaatactt cggtttgatc agttccagca
     2101 tgagcttcag ttcttcctgg ctggcatggc cggatacgtg gattttctta tcccggccgg
     2161 cgatgacatt ggcaccgagg cgcatgaggt tatcaatggt gcggctgaca ccggtttcat
     2221 tgcccgggat aggcgtagcc gaaatgatga ctgtatcgcc aggcatgatg ctgacgctgc
     2281 gatggttgtt ggaagccatg cgcgacaggc cggccatggg ttcgccctgg ctgcccgtcg
     2341 tcaagatgag gatctggtca tcgggataac ggttcagttc atccggttcg atgaggacac
     2401 cttcgggaac gtcgagatag cccagttcaa tggcaatctg gacgttgttg accatgctgc
     2461 ggccgaggac ggtgactttg cgcttgaact gcactgccgt attgatagcc tgttggatac
     2521 gggaaatatt cgaggcgaat gtcgccagga tgatacggcc ttttgcagcg cggaaagcct
     2581 tgcggaaagc atggccgaca gtcgtttccg attcggtgta gcccggccgt tcggcattgg
     2641 tgctgtccga catgagcagc aggacgccgc ggcggcctaa ttcagcaaat ttatggatgt
     2701 ccatctgacg gccatcgact ggcgtcaggt ccatcttaaa gtcgcctgta tggacaatgg
     2761 tgcctatggg agtccggaaa tacagggcac tggcatccgg gatggagtgg ttcgtatgga
     2821 tgaagccgac cttcatacag ccgatctgta cttcatcacc atgatgcact tcattcagtg
     2881 tataattcgt aatgtgattt tctttcagtt tcccttcgat gaggccgcat acgagcttgg
     2941 tggcgtatac gggcacattt acttcattga gcaagtagga caaaccgccg atatggtctt
     3001 catgaccatg ggtgatgacg acggcccgta ctttgtcccg gttttcaatc aagtannnnn
     3061 nnnnnctcat gtccgggatg acgaggtcga tgccgaacat gtcgtcatcc gggaaggcca
     3121 ggccggcgtc gatgacgata atatcactcc cgtactggat gaccgtcata ttcttgccga
     3181 tttcacctaa accacctaag ggaattacca tgagtttttc ttttttagcc aaaatatacc
     3241 tcctaaaaat aaatatagtt gtatccgttc caaccggagc ccggcagacc aggctgcagg
     3301 tctatcctat atgtagatat aacgaacagt aatggatttt cttccttcta tcatattttc
     3361 cgttatttcc gggactctgt caataatcta tcgtctcttt ttgtgaaaac tcttaacttt
     3421 tcataaaaaa tttacattta ttttgccgat ttgatgactt tttccggcac ggtcagctgt
     3481 gttccctggg gcagggccgt gcaatcgatg gtcaggctga cttcgctcat tttgatgaac
     3541 gactgggcta cggccttgtc ggcgtcactg cggccatatt catcgaccag cgtcagggcc
     3601 aggctgcgca gctggtccgt caagggcagg gagatgcggg aaatgcgctt cgtcgccggg
     3661 tcgatggtga cgaccgtcga taagtcgccg cactgctgca agccctggag gactttggtc
     3721 atgacgcgga tctgttcggc cgtcatgccg tttttcttcc actgggcctg gtcggccgga
     3781 ttatagatat gggaagcgtc gaagacgacg ttgtaatcat tgccgccggc agcggtgact
     3841 tttttcacgc cagccaggac attgtagtcc tgacgcaggt aatcggccaa gggcttgctg
     3901 tccttcaaat catagctctt cttttcccat tcatcgccat cttcctggta atagacgtcg
     3961 atttttttcc cgttctggac ggcataggct ttcccttcag ggactttctt catcgtcgtc
     4021 gcagcaaaac ccgttacctt agcctgggac tggatgacga aaggccgttc ctggacgtcg
     4081 atattgttga cgacggcccc ttcgccgaca aagggcatct tgaggcccag tttcagcgta
     4141 taagagccct gaggatgggc cgccatgttg cgggccgctt cttcatatgt tgcctgcggt
     4201 gaagcagcca gtgccatacc gccgatcgat gcagctgcca tgaccaggca gcacattttc
     4261 ttccaagaat gtgtcaatct cattttgtcc accctgcttt cttatataat tcataaaagt
     4321 gatgaatcgg gccatggccc ttgccaatgg gttcagcatg ggcaatgcct tcaaagacgt
     4381 actctttcgc cgcaccgact gcgtctggca gactcaggcc attggccagg ttcgaggcga
     4441 tggcgctcga taaggagcag cctgtcccat gggtactcgt gctttccagg cgtttcccct
     4501 tataataatg aaaatcatgg ccgtcgaaga ggatatccgt cgcatcggca atgcgatgac
     4561 cgcctttgac caggacggct ttggcaccca tggcggcaat ggccttggcc gcttcttcca
     4621 tgtcgtgcag gctttcaatg gatttccctg tcagcacttc ggcttccggc agattcggcg
     4681 tcaggacatc ggccatggga aggaggactt ccttcagcgt ctgttcggct tccggtacca
     4741 gcaggcgatg gccgctggta gcaaccatga ccggatcgat gacgatatac ggcggctggt
     4801 attgtttcaa tttatcggcg atgacgcgga tcgtttcagg tacactgacc atgccgattt
     4861 tgacggcgtc gacgtcgata tcttcaaaga ccgcatcgat ctgcgaagca atgaggtccg
     4921 gcgtgatgtc catatagccg cgcacgccct gggtgttctg ggaaacgacg gccgtaatga
     4981 cattcaaggc aaagcagccg tgggcacaaa aagtcttgca atccgcagcg gcaccggctc
     5041 cgccagaagg atccgtcccg gcaatggata atacatgttt catcatgagt acaacctcca
     5101 atttattctt tgtaagaagg tcccaaaaat ttcaaggcct tcggatggac atagaaaagc
     5161 agcgatgccg aaccgatgcg gccgccgtgg acgtcgaagt cgaacagggc catgctgccc
     5221 gtcttgaagt cgacagccgt ccccgtccag gcctgagccc agcggtcaag gaacggcgaa
     5281 tggccgatga tgcacacgac atcagctgtt tcccggctca aaatctcgtt gtatacggcg
     5341 actaagtcac cagaagcgat ggctttatgc gtatggaact gctcaatagg cagatgcttt
     5401 tctaaataag cggctgtctg acacgtccgc acgtatgggc tgacccacag ggccgttttc
     5461 cccgacggcc accatgacga ggcccatttc gccatggacg aagcctgatt acaccctttt
     5521 tcagtcagtt cacgcagttt atccggctga tgcagatcaa aaggctgggc ctttccgtga
     5581 cgcattaaac aaacatacat aaactactcc ccttttatgt acccctttta ttcggcaaca
     5641 tgctccaggc cgacgtcgag cagcgtaaag acatgggcat cggccagcga atagtatacg
     5701 gtcttgccgt cccggcggaa ctggaccagc cgggcatcgc gcaggatgcg cagctgatgg
     5761 gacacagcag actggcccat accgagttca tcggcaatat cccggacgca gacttcctga
     5821 ttcatgagga cgcgcaatat gcgcagccgt gtcggatcgc cgaggacttt gaagacatcg
     5881 gccaaaggct ggatatattg ttgtgaaagt tccggtgctt gtcgtttcaa tgcaatcgct
     5941 cctaatgctg tttatcactg tctgtcttaa tcgttttatt atagcacact tttcacatat
     6001 actgctagag gcgtcacaaa taaggcccgc acagggtaca gacaaggtaa atgaattata
     6061 aaggaattat aaaaagtcct aaaaaggcgt gaaatgtcct ggcaaaagcc tttcctctaa
     6121 atcaaaaatg ccctataatg ataacaaaat cgcaacaaaa tacttttact taggagtgaa
     6181 tgtcatgaat gaaaccatcc ataccctcat cacgcggcgc agcgtccgca agtacaaaaa
     6241 ggcccagatc agcgatgaag acttgtatct gattctccag gccggcacct atgcagccag
     6301 cggtcgtggc ctgcagccct gtaccatcgt cgtcatccag gatacggata ccctggcccg
     6361 cctgagcgct ctcaacgcca aaatcatggg cacgacgaat gaccccatgt acggcgcccc
     6421 gacggtcctc ctggtcctgg ccgacatgga aaaatccccc aacgcctggt ccgacggctg
     6481 cctcatcctg ggcaatctca tgaatgccgc cgcttccctg cacctcggct cgtgctggat
     6541 caaccgggcc aaagaagaat ttgaaacgcc cgaagggaaa gccctcctca aagaatgggg
     6601 cctgccggaa cacctcgtcg gcgtcggcca ctgcatcgtc ggctacggcg acggcccgga
     6661 accggccgca gccccgcgga aaccggattt cattaaattt gttaagtaaa tagtggttag
     6721 tggctcgtag gggcgccccg tggggcgccc gcagaccgca gttcgcaggc cgcagttcgt
     6781 ggctctcctc ataggagagc tgtcagcaaa gctgactgag aggttgcttt ttctttttta
     6841 taaatttaat aacaaccact actagaaaag ggactatcgt atgaaggctt ataccatcat
     6901 gcgtagtccc ttttctagta aggcccgcca cgtgggcgac ccgttaggaa tttgggtgat
     6961 gctcctagca aatgaccact aaccactaac cactaaccac taaccactaa ccactaacca
     7021 ctaaccact
//
LOCUS       DAWABZ010000096         6961 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2905_length_6961_cov_3.555459, whole genome shotgun sequence.
ACCESSION   DAWABZ010000096 DAWABZ010000000
VERSION     DAWABZ010000096.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6961)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6961)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6961
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2905_length_6961_cov_3.555459"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1387 a         1815 c         1968 g         1791 t
ORIGIN      
        1 ggacaggcat aacgttgttg agagcctgtt cgaatacttc caaaggatct ttattcattt
       61 tagaacggat gatgtcgaaa gcatcataaa cgatggtttc agcgacacct tttttgccgt
      121 ccaacataac tttgttgatg aagcgcgtta ccaatttgga atggtacacc ggatccggca
      181 gcacatcacg cttcggcacg gggccttttc ttggcatgtc taattcctcc tctatgctat
      241 gtaagtcatc ttgttcatct taaaaggcaa atcttatttg cctttcttag cgccgtattt
      301 agaacgggac tgctgacgat ctgctacgcc agctgtatcc aaggcaccgc ggacgatgtg
      361 ataacgtaca ccaggaaggt ctttgacacg accgcctctg attaatacaa cactgtgttc
      421 ctgtaaattg tgaccaatgc cgggaatata agcagttact tcgatggagt tcgtcaagcg
      481 aacacgggca actttacgca gagcagagtt aggttttttc ggggtagctg tgtacacacg
      541 agtgcaaaca ccgcgtttct gcgggcagtt tttcaaagcc ggtgctgtcg attttgtgac
      601 ggcaacctga cgtcctttac gaaccaattg gctgattgtt ggcatttggg cacctcctct
      661 ccaaacaatt agatttataa tgtaatcatc aacgagcaga gctcatgaga tgatgttacc
      721 gaaaattatt ttaacaagcc gacggctgca gcttttacct tgatgtggca ggccatgccc
      781 agctccgtca tggtatacgc gcggtcgaca gggatgtgag cgccttcggc ttctgcaata
      841 agcggcgcta cgacctgttc atcgcaatca ttggcgatgt acagttgtgt gacttcgttt
      901 cgtttcattg ctttcagggt ttgcttcgcg cccacggtct tgcggacact ggcagctagt
      961 tccaagctca cgaaacctca ctccttacgt cttgcaattt agacgcaata ctttcattgc
     1021 gacacttaga tattttatca tcacgcttat tatatgtcaa tgagtttttt ggtaaaaact
     1081 gcgtaatcag tacatttttt attacacgtg tcctagtgat tcgtgagcca cgagcctttc
     1141 catatataca aaacgggctc gccacgtgcg agcccctacg aacgacgaac cactaacaac
     1201 aaaaaagggc tgtcgcacat gcgacagccc ttttttactc tcttaatgtc cggcttccgg
     1261 tgtcgtatcg gcaccttccg gtgcgtctac ggggttgcct tcttcgtctt catagctgat
     1321 agacggaacg gtctttacga ccttgatctt gcggtagcgg ctcatgcccg taccagccgg
     1381 gatgagttta ccgatgatga cgttttcttt gaggcccagg agcgggtcga tcttgccttt
     1441 gatagcagca tcggtcagga cacgggtcgt ttcctggaag gatgcagccg acaggaagga
     1501 gtccgttgcc agtgcggcct tggtgatacc gaggagcatc ggttggccga tagcgggctt
     1561 gccgccattg ctttcgatgc gcttgttcat atcttcaaag atgttgacgt cgatataatc
     1621 gcccggcaat acatcggcat cgccggcgtc ttcgatcttc atcttgcgca gcatctggcg
     1681 tacgatgact tcgatgtgct tatcgttgat accaacaccc tgggatttgt ataccttctg
     1741 tacttcgtat acgaggtacc gctgggtagc ttcgacaccc aggatgtgca ggatgtcgtg
     1801 ggggttgatg gagccggccg tgatgcggct gcccttttcg atgaagtcgc cttcatgtac
     1861 gacgaggtgc gaaccgaacg ggatggtgta gctccgttct tcaccgtcct tggaagtgac
     1921 atggacgatg ttgatatcgt gatggtcttc tttttcggta atcgatacgg taccgctgat
     1981 ttccgtgacg atagcattgc ccttcggctt acgggcttca aacaattctt cgacacgagg
     2041 aagaccctgg gtgatatcgc cggccgttgc gacaccgccc gtatggaagg tacgcatggt
     2101 cagctgtgtg cccggttcac cgatggactg agcagcgatg gtgccgaccg attcgccgac
     2161 attgaccttg ccgcccgtac cgaggtcgcg gccatagcac ttcgcgcaga cgccataacg
     2221 gctgcggcag gtgaggacgc tgcggatccg gactttatca tagtattttg caatttcttt
     2281 ggcctggtct gccatgattt cttcgttgag ggcaacgatg acttcgcccg tttccgggtt
     2341 gaccagggtt tcggcagccg tacggccttc gatgcggtct tccagcgatt cgatgacgcc
     2401 gttgccgctg tcttcgacga tagccgtgac ttcgacgccg cggatgtcgt tgttgcggac
     2461 cttgacttca tcgacgtcgc tgttgaggat gcggtcgatg gcatcttctg tgagcggcgt
     2521 gcctttggcg cagatttcaa tgccgttgga atcgatgacg gctttttcga cagtcttgcc
     2581 gagcatattt tccgccatgg atttgcggat agctgcgcgg aaaccttctt ccggagcacc
     2641 gagggaaacc gtttcgatga cgttggtgtg gttcaggtca tcgttgatat ccatctggct
     2701 gccgcggagg gagagtttcg ggatctggtg ttcgatgacc gtatccatat cgtcgttggt
     2761 caggagatgt tctgccggga agacgatgtc gccggtttcc ggatccgcta catcacgggc
     2821 caggacgcgg ccgatgagct tgtcgcgcag gaggttgaca gcctggcggc acgagctggc
     2881 cagacgggcc cgttcgcgga cgaggtcgat gccgaatacg tcgcagtcat cttcacggac
     2941 gatgacgtcc tgagagacgt cgacgagacg acgggtcaga taacccgagt cagctgtacg
     3001 cagtgccgta tcggccaagc ctttacgggc gccgtgagac gaggtgaagt agtccagtac
     3061 ggacaggcct tcacggaagt tcgctttgat cggcaagtcg atgatacggc ccgacgggtc
     3121 agccatcaag ccacgcatac cggcaacctg acgaatctgg tcacggctgc ctcgagcgcc
     3181 ggagtcagcc atcatgaaga tcgagttgaa cgggtccatg ttatccatca tgacatccgt
     3241 aacgtcgtta gtggcctttg tccagatttc gacgctcttg ttatagcgtt cgttttcggt
     3301 aatcaagccg cgctgataca tggcataggc gttctggacc ttcttgtcgg cttcattgag
     3361 gatatcccat ttttctgccg ggatctgaat atcagaaata gcgatggaca taccggcctg
     3421 gcgggcgtaa tggtagccga tggccttgat cttgtccaga agttctgccg tcttggtatt
     3481 gccgacgagg tggaagcagg catcgacgag tttgccgatc tgtttcttgc tcatgaggac
     3541 gcccaggcag ttgacgtcga catattcttt atctttcttg aattcagcca cagcggcttc
     3601 gtcgatctta cgcttcggca aggggcgcgt agccgtgagg tcgacaccga atttttcttt
     3661 cagctgcgag aaaccatagt cgcggatatg gccttcttcg tctttgatct tgacgaagtg
     3721 ggaccagtcg atgaggccgt tttcataggc gaagaccgtg tccttcgggt ccgtgaagac
     3781 catgacttta tccgtatgga agaggcgcag tggttcatag atctgatagt tgtagagcag
     3841 tttgcctgcc gtcgtttcga tgaggccgtg gccagggatg cggaccttga tcagggcttc
     3901 gatgtcgatg actttggcat cgtaagcgag catgacttca tcgtagttgg agaaaatctt
     3961 gccttcgccc ttggcagccg gtttgaccag ggtcaggtaa taagcaccga ggaccatgtc
     4021 ctgtgtcggg acggcgaccg gtttgccatc tttcggagcc aggatgttat gagacgaaag
     4081 catgagcgtc cgcgcttcgg actgggcttc tacggaaaga ggcagatggc aggccatctg
     4141 gtcaccgtca aagtcggcgt tgaatgccgt gcaaaccagc ggatgcagtt tgatggcgcg
     4201 gccttcccag aggatcggtt cgaaagcctg gatacccaga cgatgcagtg tcggggcgcg
     4261 gttcaagagg acaggatgtt ccttgatgac gtcttccagg acgtcccaga cttcactgtt
     4321 gacgcgttcg actttcttct tagcgttctt gatattggta gcaatgcctt tttcgacaag
     4381 tttcttcatg acgaaaggct tgaagagttc cagggccatt tctttcggca ggccgcactg
     4441 gtgcatcttc atttccggac cgacgacgat aaccgaacgg ccagagtagt cgacacgttt
     4501 accgagcaag ttctggcgga aacggccctg tttccctttc agcatatccg acagggattt
     4561 caggggacgg ttgcccgggc ccgtgaccgg acggccgcgg cggccgttat cgatgagggc
     4621 atcgacggct tcctggagca tacgtttttc gttgcggacg atgatgtccg gtgcgcccag
     4681 ttccaagagg cggctcaggc ggttgttacg gttgatgacc cggcgataga gatcgttcag
     4741 gtcagacgta gcgaagcggc cgccgtcgag ctgtaccatc gggcgcagtt ccggcgggat
     4801 gaccgggatg acatccataa tcatccattc cggtttgttg ccggagtggc ggaaggcttc
     4861 gacgacttcc agccggcgga tagcgcggac gcggcgctgg cccgacgaat cgcggagttc
     4921 atcgcggagc tgtttgtcca gctggtccag gtccaggccc tgcagcagtt ccttgacagc
     4981 tgcagcgccc atgccgacgc ggaatttatc gccgtacata tccagatact gacgatattc
     5041 cgtttccgtc atcagctgct gcttggtaag cggcgtatcg cccggatcga ggacgatgta
     5101 cgaggcgaag tataagactt tttccagcga acggggagaa atatcgagga tcaggcccat
     5161 ccggctgggg atgcctttaa aataccaaat atgagatacc ggtgtagcca gtttaatatg
     5221 gcccatccgt tcacgacgga ctttggcgcg cgtgacttcg acgccgcagc ggtcgcagac
     5281 gacaccttta taccggatac gtttgtattt gccgcagtga cattcccagt cctttgtcgg
     5341 cccaaaaatg cgttcgcaga acaacccatc ccgttccggt ttcaatgtac ggtaattgat
     5401 cgtttctggt ttcttgactt ccccgtggga ccattcgagg atcttttccg gtgaagcaag
     5461 accgatccgc atggaatcga attcattcac atcaagcact aataccgctc ccttctatcg
     5521 actcgttatt catcatcgtc agcctgttca gcttcggcga cggcatcgag gctcatggca
     5581 ctcatgatgt catcttcgcc gccgttggcg acgggttcct gttcttcgcc ttcttctcct
     5641 tcggcaggag tgtcttcagc cggtgcagcc ggtgtttccg gaccattttc accttccatg
     5701 acgcggcgga gttcatcagt ctgttccggt tccgtttcat cgtcgagttc cttgatgacg
     5761 acttcttcgc cgtcttcgtt caggatctgg acgtcgaggc cgatggcctg gagttctttg
     5821 aggaggacct tgaacgattc cggtacgccc ggttcaggaa tattttcacc tttgacgatc
     5881 ttttcgtaag ccttgacacg gccgacgaca tcgtcagact taacagtcag catttcctgg
     5941 agggtatagg ctgcgccata cgcttccaga gcccagactt ccatttcgcc gaaacgctgg
     6001 ccgccgaact gggctttacc accgagcggc tgctgcgtga caagggagta cgggcctgtc
     6061 gaacgagcat ggatcttgtc gtcgacgagg tgatgcagtt tgaggaagta cgtgtaaccg
     6121 acagtgacgc ggcggtccat cggttcccct gtacggccat catagaggac ggatttgccg
     6181 tcttccggca ggccggcgat cttcagggct tcaaagacgt cttcggcctg agcgccgtcg
     6241 aagaccggtg ttgcgatatg gacaccggcg acgtccggtt tgggcatgcc gtatttatcg
     6301 aaatcatagc cggcagcgcg gagtttttct tccaggcccg gatccatttc cttgatctgt
     6361 ttgcccagtt cgtggacagc ccagccaagg tgggtttcca aaatctgacc gatattcata
     6421 cgagacggta cgcccaaggg gttcaggacg atctggaccg gcgtgccatc gggcagaaaa
     6481 ggcatatctt cctggcgcat gacgcgggaa acgacacctt tgttgccgtg acggccagcc
     6541 atcttgtcgc cttcgtggat cttacgtttc tgggcgatgt agacgcggac caggtggttt
     6601 acgccaggcg gcagttcgtc gttattttca cgggagaaga tgcggacgtt gacgactttc
     6661 cccgattcac catgaggtac gcgcagcgac gtatcgcgga cttcgcgttc cttgtcgccg
     6721 aagatggcgc gcagcaggcg ttcttccggt gtcagttccg tttcgccttt cggcgtgact
     6781 ttgccgacca ggacgtcgcc cgggaagacg tcggcaccga tcatgatgat gccgttttcg
     6841 tcgaggttct tcgtgctttc ttcactgacg ttggggattt cacgggtaat ttcttccgaa
     6901 cccagtttcg tgtcgcgggc atcacattcg tattcttcga tgtggatgga tgtgtagagg
     6961 t
//
LOCUS       DAWABZ010000097         6952 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2913_length_6952_cov_3.459475, whole genome shotgun sequence.
ACCESSION   DAWABZ010000097 DAWABZ010000000
VERSION     DAWABZ010000097.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6952)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6952)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6952
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2913_length_6952_cov_3.459475"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1409 a         1890 c         1948 g         1705 t
ORIGIN      
        1 ggccttatcc gggcggtcag cgcgggggat ttccaggaat tcgtcatcaa agagttcccg
       61 ggcttcacag attttccagc catcctggaa tgtcagttca cgaatcaagc tgctcttgcc
      121 ggaaccgggt ccgccaatga gaatcaacaa tttttcatcg tcctgggcaa tgtacttcca
      181 ccgttttaaa acatctgcat ctgtaaccat gaaaatcacc ttcgttttct tatattattt
      241 attttattat acctaacttt catcatataa gtaaagcatt tttgctattt tattttgaca
      301 tatgacaaaa caatatgtat catcatttca atccataccg ttccatcggg tcctggatgt
      361 tcttgatcgg cgtcgacgtc aggatgatgt catcgacata gggctcgttg tgacgcacga
      421 cgatgaccag ggacacgcct ttctgatagg gactgtcata gtaataggcg tcgacggatt
      481 tttctttgtc cggcccccat tcgacgcggt ccggccggcc atataaggcc aggatcgtcg
      541 agacatgata acccagggtc gccccgcggc cagtataggc attattcgtc aagaggaaga
      601 ctttctgcac gatattgcgc cggtccactt cgacgccgta atcgccatag agccagaagt
      661 tgctgttatt gcgcttgacc aggttggtcc acatgttgta cttgttcggc tcgaaaggct
      721 ggttgaggcc aaaacccatg agggtaaagt cccgttcgcc cagtttcatg cggtccgccg
      781 gttcgtcccc cgtaatgtac ggcggccgca cgggcatgag ggccacgcgt tcgatgcggc
      841 ggttgccgat ggcaaagacg tacatgacat ctttccgcgg actttcataa gtgaagtaat
      901 agacattggc atcggcatcg cgcaggaccg aatcgggcag gccatattgg cgcagcagca
      961 cttcggccgg atatttcagg cgtacgtccc ggccgattag gatgttcgtc ccagccgaca
     1021 ggaacatcga ttccgcaccg ggcaggacgg ctttgcgcgt atcggcactg tggcgcaggc
     1081 tgtcagccgt cttgttgcgg aaagtaaagc gcatcttcaa gtccttgtcg ttgtactgca
     1141 tggtcgtata gtgggtgctg cgggaaaact tcgccggcgg cccaaaaatg cgcttgacct
     1201 tggccggtct gtcgccgtga tggatgctgc cgatgtaaaa atcactggcc tgcaggggcg
     1261 gaatggtcag cgtatccagc gaatcgagga ggacgcagtc atcatccgtc ggtacatggg
     1321 ccgtttcttt tttgctgtct ttattctctt tgccgtcttt atcggattta tccgctttgc
     1381 cgtctttatc ggatttatcc gctttgccgg ttttgtcatc ggactggggc gcgacggcct
     1441 tgatggcctt atcggcgtcc ttctgggcag acggaacggt cccggctttc ccgccggcat
     1501 ccactgcggc tgcagtggca gtcccggtca gggccttttc cggcttttca gctggtgccg
     1561 cccccgttgc cggcccccag gccaggcagg cagcgaagac cagcgcagcc atggctgtac
     1621 atttcatagc gttcctcctt cgtaggattg agaaaacgga gctgtccaat gaagacagct
     1681 ccgttttacg ttggtcgcag gccgttcatc gtttgtcgca agggagcaaa aataacaagc
     1741 catcgcgatg aacgatgaac gacgaacgaa aaacttttaa gtcaaaggaa tcgtgcatga
     1801 tggcagaaac cttcatggaa ccgtccttat cttatttatg ctgttccggg cggctcaacg
     1861 ggaagaggag aacgtcgcgg atagacgggc tgttggtcag gaacatgacc agtcggtcaa
     1921 tgccgatgcc caggccgcct gtcggaggca ggccatattc cagtgcgcgg cagtagtctt
     1981 cgtccatctg atgcgcttcg tcatcgccgc gttcccgttc cttcatctgt tcgatgaagc
     2041 ggttgcgctg gtcgatggga tcattcaatt ccgagaagcc attggccagt tcgcggccat
     2101 agatgaaggc ttcaaaacga taggtcaaga gcggattgtc ccggtcctgt ttggtgagcg
     2161 gcgagatttc caggggatga ccagtgatga tcgtcggctg gataaggttt tcttcgacga
     2221 atttttcaaa gaaagcgttg atgatcttgc cgacgccatc gtactggccg tattcaacgt
     2281 cgtgttcgtc agccatcttg cgggccgttt cgacatcggt aatgccttca aagtcagcac
     2341 cgctgaattt cttgacggct tcgatcatgg tcatgcggtc ccacggcggc gtgaggtcga
     2401 tttccttgtc gatataggtc aatttcatcg tgccatagag catttctgcc agtttggaga
     2461 ccatgtcttc cgtaatgttg atgacgtctt ccaagtcgcc ataagcctgg tacaattcga
     2521 ccgtcgtgaa ttccgggtta tgccgtgtat cgatgccttc gttgcggaag cagcggttca
     2581 tttcatagac ccgttccaag ccgccgacga ggaggcgttt gaggtggagt tccggtgcga
     2641 tacgcaggta gatgtccata tcgagggcgt tgtgatgggt cttgaacgga cgggctgcag
     2701 cgccgccggc gatggtatgg agcatcggcg tttcgacttc caggaagtgg cggtcatcga
     2761 ggtatttgcg gatgcccttg atgatcttcg accgtttgat gaatgtgtct ttgacttccg
     2821 gattgacgat gaggtcgagg tagcgctggc gataccgcgt ttccttatcg gtcaggccat
     2881 ggaatttttc cggcagcgga cggagcgatt tggacaagag ggtccatttt tcgacgcgaa
     2941 tcgtcgtttc gcccgtatgg gtgacgaaga ctgtcccttc gacgccgacg atatcaccga
     3001 tatcgaggag cttgaataaa ttgtacgatt cttcaccgag tacgtctttg cggaaataga
     3061 gctggatttc gccgctcaag tcgcggagga cgcagaatgc agtcttgccg tggcggcgga
     3121 tggccatgag gcggccggcg acgctgacga cggtttcatt cttttccagt tcaacggcgt
     3181 catctttgac ttcctgtgca tgatgatccc agttgtattt ctgaccgaag ggatagacgc
     3241 cggcgtcgat gaattcctgc atcttttcgc ggcggacctt catttggtca ttcaattttt
     3301 cttcttcaat aatgggcgtt tctttttctt tcgtgtccga catgatattc ctcctaggat
     3361 ttattctgca tcaggcggca agatgcggta tttgatttca cctgccggtg ctttgacggt
     3421 aacgatagcc ggattggcag catcgatgac ctggccgagg atggctttcc ctacaggaga
     3481 ttcattggaa atgcggccgt tgaacggatc ggcttctgcc gtaccgacga cggtgtaatc
     3541 gaagcggtcc cccgtttcgg catcttccag gatgactttg gaaccgaggc ggactttatc
     3601 cgagccatca tcggtttcct tgatgatctt agccgtgcgg atcttggctt ccaattcggc
     3661 aatacggcct tcgatgatgc cctgttcatt cttggcggca tcgtattcgg cgttttcgct
     3721 caagtcaccg agggcgatag cttctttgat gcgctgagaa atttcaatgc ggcggaccga
     3781 tttcagttca ttcaattcat cttttaattt ctgtaaccct tcctgggtaa tcagcgtttc
     3841 gttggtcatg atattcctgc tcctcctgcg atataatcac gtttcaatat catatcttta
     3901 atttataccg tactcttaat tatatgcaca tccgtccctt gtgtcaataa aacagctatg
     3961 tccctgcccg gcgcgccttg tccatcatgt cgcggacgat ggctcggaat tcgtcagccg
     4021 aaccggcccg gttgatgcgt tcgcgcaggg ccgccgagcc atagaggccg tgggtatacc
     4081 acgaggcgtg ggagcgcatc tcccggacgc cgatgtattc acctttatag gccagcaggg
     4141 cttcaaaatg ggacagcatc tgttcataac gttccatggc cgtcggcggc ggcaggactt
     4201 cgcccgtcgc caaataatgg cgtatctgcg ggaaaatcca gggattgccc tgagcgccgc
     4261 ggccgaccat gacggcaccg cagccggtct gttccatcag ggctgccgcc gacgggccgt
     4321 cgacgacgtc gccgttgccg atgaccggga tggatacggc atcgacgacg gctttgattt
     4381 tctgccagtc cgccttgccg ctatagaact gttcccgcgt ccgtccgtgg acggtgacgg
     4441 cagcggcacc agccgcttca gcccgtttgg ccagttccgg cgccgtaaaa gaggcgtcat
     4501 cccagccggt gcgcatcttg accgtgaccg ggatggacac ggcgccgacg acggcacgga
     4561 tgactttctc tgccaggggc aggttcttca tcaaggccga gccgtcgccg ttcttgacga
     4621 ctttcttgac gggacagccc atattgatat cgacgatatt ggcaccggct tcttcgacga
     4681 cggccgctgc ctgggccatg acgtccgggt ccgagccgaa aatctgcatg gacagggggt
     4741 gttccccgtc ttcgatgtgc agcagttctt ctgtatgctg gttgcggtag cggataccct
     4801 gggaactgac catttccgcg cagaccaggg ccgccccata gcggtgggcg atgacccggt
     4861 acggcgcatc acagacgccg gccatagggg ccaggatgac cggggcatcg agctcgacct
     4921 tgccgatggc aaagggctta acggttgcgg tcataaatga ttctcaaccc ttccagggtc
     4981 agcatgggtt cgacgacgtc gatggcatcc gtagccggag cgacgatgac ggccaggccg
     5041 cccgtagcga cgactttagc cttacagccc agttccttgc gcatacgggc gacgatgccg
     5101 tcgacttggc cgacgaagcc atagaagacg ccggcctgca tactttcgac ggtattgcgg
     5161 cagatgacct tatccgtgcg gacgacttcg atacgcggca gtttcgccgt gcgctggacc
     5221 agggcgtccg tagagatgcc gatgcccggg cagatggcgc caccgaggta cgtccctttc
     5281 ttgtcgacag cacagaaggt cgtcgccgtg ccgaagtcga tgatgatgac cgggccgccg
     5341 aatttttcat acgctgcgac ggcgttgacg atgcggtcgg caccgacttc acggggattg
     5401 tcgtatttga tgtccatgcc cgtcttgaca cccggtccga tgaccagcgg cgtcaggccg
     5461 aaatagcgct ggcacatgcg ttccagcgtg ggcatgaccg gcggtacgac cgacgaaatg
     5521 atgatggcat cgatttcatc ggagttgacg ccgttcaaat aaaagaggtt gcgaatgagg
     5581 acgccgtatt catcggtcgt ccggaggcgg ttcgtcgata cgcgccagtg gtccatgagc
     5641 tcttttccct tgaaaatgcc caggacaata tttgtattcc ctacatcaat gactaatagc
     5701 ataactttat ctcctgccta ctttaagttg ataatacgcc tattataata acagagtttg
     5761 cctgtcttga atagtcttta cggctaaatt aagccggacg gacatgaata tcccctgcca
     5821 tgacgacttc cacgccttct ggcgtcgcga cgaggagatt gccgttctca tcgatatcga
     5881 cggctttgcc ggaaaaggtc ttgtcgatgg atgtgaccgt tacgtcctgg cccaacatgc
     5941 aggacagggc ccgccattcg gccaggactg gggcgaaacc gtcacggcag acggtctggt
     6001 acagcccttc gatttccttc agcagcgttt ccagcagggc cagccggctg acgcggacgc
     6061 cttcgcgggc aaaactggtg acgatggact gcaaatcctt aggcaggtcc tcagcggtga
     6121 tacccgtatt gaccccgata cccatgatga tgtaattgac cttttccgcc gaaccgttta
     6181 gttcggtcag gatgcccacc atcttgcggc ccttgaccag gatgtcatta ggccacttga
     6241 tgccgcagtc cgtcaggccg tgatggcgga tggcccgggc cacggcgacg gcagccagga
     6301 gggtcatctt ggagacttcc ataggcggga tgttaggccg caggatcagg gagaaccaga
     6361 cgcccttggc aaaaggcgac aagaacgagc ggttaatgcg tccccggcct ccggtctgtt
     6421 cttcggcgac gacgatggtc ccttccgggg cgccttcatc ggccagcttc ttggcgacat
     6481 tattcgtcga atcgacgcgt tcccggtaga cgatatgacg tcccacgact ttcgtcgaaa
     6541 gcccttcttc gatagcctgt tccgacagca gttccggcgc ttcgcgcaga caataccctt
     6601 tcttggtata cgactcgatg acatagcccc gctgtttgag gacgcggata tgcttccata
     6661 cggccgtccg ggaaatcccg agctgctcag aaatctgctg gcccgatacg aattcgtcct
     6721 tgtgctgtat caaaaaatcg agaatgtctt gtctcatagc aatcacctct gccgctgccc
     6781 ctggcagcat ccattcaaaa aaaagcgcta ctccctagcg cataactcac ctattctatg
     6841 tagaaattgt aacataaacg ataaccctgt caaggggagg ttagggttag tggctcgtgg
     6901 agagcaccac caaatcccag gcgggtcgcc cacgcggcga cccctactac aa
//
LOCUS       DAWABZ010000098         6933 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2922_length_6933_cov_3.552922, whole genome shotgun sequence.
ACCESSION   DAWABZ010000098 DAWABZ010000000
VERSION     DAWABZ010000098.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6933)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6933)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6933
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2922_length_6933_cov_3.552922"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1543 a         1905 c         1959 g         1526 t
ORIGIN      
        1 tcgcacggct gccggtccag cgcctttccc ctcatttcgg caggcatctt tggctacccg
       61 aaagcagaag ccttggatgt cgcccgggaa gcggcagcgg ctttcttgaa aaatcatgac
      121 atggatattt acttggtctt atatccctag gaaaggaaaa cattgtgatt cgcaatattg
      181 tacatgacgt catcttcttg ggccgtaagt ccgaagcggc gacgaaagat gatttgcccg
      241 tcgtccagga cctgctcgac acgctgaagg ccaaccgcat gacctgtgtc ggcatggcgg
      301 ccaatatgat cggttctttg aagcgcatca tcgtcgtcca tacggatagg ggcgacgaaa
      361 ttttcatcaa cccggtcatc gaaaagaagg ccgatccttt tgaggccgaa gaaggctgtc
      421 tgtccctggt cggcacgcgg aagacaacgc gctaccggaa gatacaggtg ttctaccagg
      481 accgccgctt ccgcaaacac cgccagacct ttaccggctg gacggcccag atcatccagc
      541 acgaatgtga ccacctggac ggcatcatca tatagaaagg acgcctgcag gacatccgca
      601 ggccgcaggg tatcgcacga aggtttctgc catcgggctc ggtccctttt gttttttaat
      661 ttaaaatcct tcgctaacca ctaacaacta gctactaaca acttaaaagg ggttgtcgtc
      721 atgcggcaag ccctttttgc gcgcaatatg gtataatagg ctagtggcta gtggctagtg
      781 gctagtggct agtgggccgt aggggccgtc ctgaaaggcg gcccgttggg gtcaccttgg
      841 atttatgagt acttttcaag aaagtgagac aatgatgaat cgatcgatga ttgcgaattt
      901 gatgaagcct ttgacgctgg ggctgatcgt ggccttgttt ttggcgatcc actgggccat
      961 tccttcgttt tacagcacgt tatggcagct gacggtgagc catgatattc cggggctgac
     1021 gtcgtatatt tcttcttttg gcagtattgc cgtggtcctc atgatcctgc tcatcgtcct
     1081 gaccaacatg accggcctgc cgtcggtcca gttcgtcacg gtcaatggca tcttgttcgg
     1141 cctcctgccg ggcatcctca tttcctgggc tggtcaggtc atcggcaata tcctggcctt
     1201 tgtcatcatg cgctatgtct tccggcgcaa ggcacaggtc ctggtcgata agagccgctg
     1261 gctgtccaag ctgaaccgcg gtatcgattt ccggcttatc tttttcttcc gggccattcc
     1321 ctattctccg aatttcgtcg tcaccgccct ttgtgccctg agccgtgtcc gcttcggcga
     1381 acatgcggcg gcgacgatga ccggcaagtt cctggccgtc tgtatggaag tctggctggg
     1441 ttacgacctc atccacttca atccgcacgg cctcatcgta gccggtatca tggtctgtct
     1501 gctggcagcg ggtctctttg gcagccgccg ctggcagaag aaagacgaaa aatagacatc
     1561 ttatcatatt atatataatg ggagggattc ttcatgggtt tgatcaaagc agctatcggt
     1621 tctgtcggcg gcgtcctggc cgaccagtgg aaagagtttt tttactgtga cagcctgaat
     1681 gaaaatatct tgatggccaa aggtcagaaa cggaccagcc gtcatggccg cagcggcaat
     1741 acggacggcg aagacaatat catttccaac ggctcgcgca ttgccgtcaa caacggccag
     1801 tgcatgctca tcgtcgaatc ggggaaaatc gtcgaagtct gcgctgaacc gggcgaatat
     1861 acatacgatg cgtcgacgga agcgtctatc ttcgacggcg actatgccgg tgacctgggc
     1921 gaatcgttcc gcctgctcgt ccgccagatg aaggaacgct ttgcctttgg cggcgatacg
     1981 ggcaaggacc agcgcgtcta ctatgtcaac cttaaggaaa tcatcggcaa taaatacggt
     2041 acgcccagtg aagtgccctt caagtcttat gacgatacga cgggactggc cctgctcatg
     2101 cgcctgcgct gcttcggtga atacagctac cgcatcgtca atcccatcct gttctatacg
     2161 aacgtggccg gcaatgacgt cgacgtctac gaacggagcc gcctggacag tcagctcaaa
     2221 tcggaactct tgacggccct gcagccggcc tttgccagga tttcagcctt gcacatcgac
     2281 tattcccagc tgcccggcca tacgccggaa ctggctcagg ccctgaatga agtcctgtcg
     2341 ccgaaatggc gcgacctgcg gggcatcgaa atcgtatcta tcggcatcaa cagtgtcaaa
     2401 gccaatgaag aagacgaaga aaagattcag aaagctcaga tgggcttgac catgtctcgg
     2461 cccgatatcg gcctggggac cctcgtcgat gcgacggccg attccatgcg cacggctgca
     2521 gccaatgaag gcggcatggg tgctgccatg ggctatatgg gcatgaatat ggccaatcag
     2581 gccggcgccg gcctggccca ggaactggca gcccgccagg cggcccaggc gccttcgccg
     2641 atgccgcagg ctgcgccggc cggcagctgg acttgtgcct gcggtcagtc cggcaacacg
     2701 gggaacttct gcacggcctg cggccgtccc aaaccgcagg ccgccgctgc ctggacctgc
     2761 ccgaaatgcg gccaggccgg gaatacgggc aacttctgca gcaattgcgg cgcgccgcgg
     2821 ccccaggttc cggccaactg gacctgcccg tcctgcagga ctgtcaatac ggggaatttc
     2881 tgtatgaact gcggtgcaaa gaagccggaa aggtaaggtg agggtcatgg cggatacatc
     2941 ggtcagctat caatgcccga actgtggcgc accgctgcaa ttcaagccga aagacaagac
     3001 cgtcacttgt gactattgcg atacgaagtt cgacgtccag accctggaag ccatctatgc
     3061 ccagaaagaa gcgcgggctg ccgaagctga tgcggcccgc aaggtccgtt ggaagacgga
     3121 agcggcaggc caggattgga gcgacgacga agcggctcat ttacagacct acacgtgttc
     3181 gtcgtgtggc gccgaaatcg tctgcgatga gaataccatg gccacagagt gctgttactg
     3241 cggcaatccg actctcattc ccagccgctt ttcagggatg ctcaagcccg attacgtcat
     3301 tcctttcaaa aagacgaaag acgaagccgt agcggcgctg aagaagttct atcaagggcg
     3361 ctggctcctg cctaaggcct ttacggctga caaccgcgtc gaagccatcc agcccatgta
     3421 cgttccgttc tggctcttcc atgcccaggt cacggcgtcg gcagctttcc gggccgaaga
     3481 cgatgccgtc tttgagacgc cggacgaaac gattatcgaa accagcatct accactgtga
     3541 ccggacgggc tctctggcct tccatcgcat ccctgtcgac ggcagccaga agatggacga
     3601 tacatatatg gaaagcatcg agccctttga ctacggcgaa atggtcccgt tcagtccggc
     3661 ctatttcgcc ggttacttgg ccgataaata cgacgtcgac gccgacgctg ccgtccccag
     3721 ggccgatgaa cggctgtcgc agagtgcctt cgccatgttg gaagagacag tcgacaatca
     3781 tcagcgccgg acatttgaag acggcgtcgt ccataaagac gaagggaccg tggaatacgc
     3841 catggcgccg gtctggatct tgacgacgcg ctatgacggc aaagcctaca ccttcatgat
     3901 gaatggccag actggcagga tggtcggttc cctgcggtat gacaaggcca agtcctatga
     3961 atacggcgcg gccctggccg ccgtcctggt accaatcctg tatttcctgg tgaaattcgt
     4021 catggtctgg tcgggcctgt tatgaaggga gtgagtacga tgtggaaatg gttgaaagaa
     4081 acaatccttg ccatagttgt cgtggtcctg acgacggctg gtgccgcagc ggcagcgccg
     4141 agcctgtccg acggcgccca tctactcacg ccggcagaac aggctgaagt gacggccgtc
     4201 ttgcaggata cagaagcaag atatggtgta cgcgtcgctg tagtcaccgt cgcctcgacg
     4261 aagggcgtca aggccggaac ctatgccaat cagctcctgg accgggacta taaggacggc
     4321 cagaacggca atatggtcct gctcctggcc atggaccgcc atgactggta tgttgcgaca
     4381 gacaaggcca tgaaggagaa aatcagcaat aaaaaaggca tcccttatct caaggatgcc
     4441 ttcctgccgg ccttgaagga cggtcattat gcgaaagcct tcgaggccta tggccagtcg
     4501 tcggcaaaac tattggctta ttaccaggaa aagggcaagg cctatgaccc ggccgatgcc
     4561 ttcagccccc tggccctggt cgtggccctc gtcctggctg tgggtggcgg cgtggctttc
     4621 cgggcctatc tcatcagccg gatgagcaat gtccgccaag ccgcggcggc tgatgcctat
     4681 gtcgacgccg gcagtttcca tctgaccgac agcagcgata cgttcgtctt catgaccgtc
     4741 cagcacattg ccaaggcccg tccggccgat gatgacctgc ctgatttcga cagtgacgct
     4801 gactgcagcg acgacgacca cggcggcggt ggcggctcgt tctaggaacg gcggtggcac
     4861 catcaggcct tgtcgtcatg gaagaattcg atgatcttgt ggatggccag ggccatgtgg
     4921 ttcgggttct ggaaggggtg gttggcccct tcgacgggaa cgaattcgat gacgttgttt
     4981 tcagcgaatt cccggacgag gtcgatggga atcatttcat ccttcgtgcc gtggaggatg
     5041 aggatatctt cggcaaaatc gaagtattcg tggtcacgga catcgaagct cttcaaatca
     5101 tccataaatg cctggtcgat tttcatcttg cgttcgaaac cgacctggat ttccttgccc
     5161 ttcttcagct tggcaaagcc ttcgtcggag acatggttgc tcatgctttc atacatgttg
     5221 acgcctgggc tgcgcagggc gattttctgg aagggattgc ccttttcgat gaggtagcgc
     5281 agggtgatgt aagcgccgaa actcgtcgaa tagttgtaga tgtgtttggc ctggagctcg
     5341 gtcttggcgt agtcgacgac ccaggtcaga tagttcatgc attccgggag ggataacttc
     5401 ttgcgggcgt cggcgccgtg gcagggccag tcgaaggcga tgacggcgta gttcttgtat
     5461 ttgctcgtca agtgttcagc gaaatggacc gtcccggccg tgtccttgtg gctgccgaag
     5521 ccgtgcgtga cgatgacgac attgtcgaaa gtgcgcgtct gtttatcatt atcattgatg
     5581 agcagcttgc agcggatgct gtagccctgc tgattgatgt caaaattctt ttccatgctg
     5641 tcattcctct ttaccctaaa ttcgtcaaaa tgacgtacgt cgttagtata gcacaaaaaa
     5701 ggcgctgtcg cacgacgaca agcccttttt cttgtagggg ctcccacgtg gggcgcccgc
     5761 tctgggatat gtacgatttc ccgacccgtt tgctagaaga attatcccaa ttcctaacag
     5821 gtcgcccacg tggcgacccc tacaagctcc gtactgcaaa ctataaagac aacctcctac
     5881 ggaccacgag ccacgaacca cgagccacta gccacgagcc actagccacg aaccactagc
     5941 cacgaaccct taccgatgca ggccatagcg gatgatgagg cggctgattt ccaggagggc
     6001 gatcatgggc agggccagga ggcactggga caggatgtct gtcgtcggcg tgatgacggc
     6061 caggaggatg atgtatttcc gtttcttggc cagcatggca gacgtgatga aatccagctt
     6121 ggccaggacg atgaggacca gtggcaggtt gaagaggacg ccgaagggca ggaccagcat
     6181 gaagacgaaa ttcaagtcat ccatacgtat gaagacgtct tctgttatga cgtccacacg
     6241 gacacggcct tagccggcca gctgcttcac gatatcatga aacccttcgt cttccagtgg
     6301 cagcccgacg gttcatccct gaaggaatat acgattgccg gccagggggc acatcatgtc
     6361 atttccatcg ctgaatcgat gtaccgcagc ctgctgccgg atgtcgtcgt cgctcaggcc
     6421 tgtgcccatg gggccccgaa tgatgctaaa ggggaagcag acgtcgtagc ttggctgaaa
     6481 gccgcgtctc tcatggctca gaaagccccc gtcgcttatg gcgtcctcaa caagatgggc
     6541 aatggcctgc catcgccgca ccgtcaggaa ggctatatcg ttcatctcgg tgaccatgac
     6601 tgggtactca gctcaccagc cggccagaag acgtcggccc tgctcaagaa aatagctgtc
     6661 cagcagtacg gcatgagcga tggcgacagc cagggtgcga agttcaatta cttcaagaat
     6721 tatgttgaat cccagttgtc ctgcatgtat ctcaacatgc tccaggccga accggacggg
     6781 gaacgcctgg tcgctcaggc tgtagccaag gtcatcgtaa aataaatcgt ttaacttttt
     6841 ataatagcag atattgacag aaaccactta aaaatttaca atgtaacaat aaagatttac
     6901 aggaataaat taaaattgta aaggaagtgt tat
//
LOCUS       DAWABZ010000099         6926 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2928_length_6926_cov_3.909329, whole genome shotgun sequence.
ACCESSION   DAWABZ010000099 DAWABZ010000000
VERSION     DAWABZ010000099.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6926)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6926
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2928_length_6926_cov_3.909329"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1644 a         1898 c         1920 g         1464 t
ORIGIN      
        1 ttttgagtat taagtttgca gtttgtagtt tttagtgaag gcttttaaat ttaaagtcag
       61 cttctacaaa ctacaaactc cgtactataa acgataaaga gaaaggaatc ttgcatgatg
      121 atagaagcct tcgtgcgata gccccttaat ctcactcttc tccgctgtcg atgtagctgt
      181 acagggtata tttggagatg ccgaagtgtt tggagatttt gtcgccggcc ttggtgatca
      241 gcagggcccc gcggtcgttg aggaatttga tggctttgat cttgtcttcc cgtgtcatga
      301 gggggacggg tttgccgacc tgacgcgtcg attcgtcgat gaggtcgttg aggaggtccg
      361 tgacattctg cgggatgcgg ctgaccttat cttccttgga cgtgaccgag atgagcggct
      421 tgatggcatt ttcgaccatc atcagttcgg taatgtcata gttgatggag aagatgccga
      481 tgactttttg ctgttcattg cggatgtaaa tggtcgtcga cttgaggatg cggccgtctt
      541 tggtcttggt caggtaactc gtatggtcct ggagcttgtc gggatcgctg cgcagggcgt
      601 cgaggacggc catagacggg ccatcgccta ctttgcggtt ggtgacgtgg ccattttcga
      661 tggcgacgat ggaattttcc ttataattat cgctcaagtc gtggatgacg atttcacagt
      721 tcgggccgaa ttggtctgcc aggcctttgg ctaagtgacg caagaagttg aggacgacgt
      781 cgtctaacat aaggggcacc tctttctcat atcaaaaaca gattattagc ttatctgcta
      841 aaaatttttt aggtactgtt actatagcat attttcggga taaatcaacg cccttggtga
      901 aaaaaggtat gtcgaaaaaa catctttcca ttacaggcta taacaaaaag ataataaaaa
      961 atttttagac taaaaaaaaa ttgacaggta aggcggaacg ggttactatt aagatagata
     1021 cgagaatgac acaaataaca tgcattaact gcaagaaatc ttatgattta tgatttgtat
     1081 agcatagagg aggtatactt atgttaatcg tcggaaacgg tcacgtcgtt acccgcgacg
     1141 cgaagaatca atatttccct gatggcgccg tagccataga cggcacgaca atctgccgcg
     1201 tcggccagac ggcagaaatc cgccgagcct atccagacgc cgcttttatc gatgccaaag
     1261 gcggtgtcat catgccggct ttcatcaacg ttcacgagca tatttacagc gccatggcca
     1321 ggggtatgaa tatcgacggc tatgcgccca agggcttcct ggacatcctc gacggcctgt
     1381 ggtggaacat cgaccgtcac ttgaccaacg acctgactta tttgagcgcc atggaaacgt
     1441 atgtcgaatg tatcaagaac ggcgtgacga cagtcttcga ccatcacgcc agttttggtg
     1501 aaatcaagga cagcctcttt gccatcggcc aggcagcgca ggaactgggc gtccgctcgt
     1561 gtctgtgcta cgaagtctcc gaccgcgacg gtcaggataa agcttgccag gcagtcctgg
     1621 aaaatgaagc ctggatccgc cacgctcagg ccgacacgac ggacatgatt gccggcatga
     1681 tgggcatgca tgcccaattt accatcagcg acaaaacgat ggccctggca gccgaacata
     1741 aaccggacgg cgtgggttac cacatccacg tcgccgaagg catcgaagac ttgcaggatt
     1801 gtctgaagaa acacggtaaa cgcattatcg accgccttta tgactggaat atcttagggc
     1861 ccaagaccct gctcggccac tgcatccacg tcaatcccca cgaaatggac ctcatcaagg
     1921 cgacagatac catggtcgtc cacaatccgg aatcgaacat gggcaatgcc tgtggctgcc
     1981 cgccgacgat ggacatggtc caccgcggta tcctgaccgg cttggggacc gatggctata
     2041 cgcacgatat gattgagtcc tataaagtcg ccaatatcct gcacaaacac catctctgcg
     2101 acgctacagc cgcctgggct gaagtaccgc agatgctgtt ctataacaac gcagccatgg
     2161 ccaaccgcta tttcaagaag cccttaggcg tactggcaga aggggctgca gccgacgtca
     2221 tcgtcatgga ttacattgct ccgacgccga tgaatgaaga aaacatcaac agtcacatct
     2281 tattcggcat aagcggccac gatgtcgtca cgactattgc caatggcaaa gtcctcatgg
     2341 aaaaccgcca gctcaccggt gtcgatgaag aagccctgat ggctaaaatc cgcgaaggcg
     2401 cagcgaccct ggccggacgc attaatggat acttataagg aggaatctat catgagtgat
     2461 cgcatgacat ccattccctt tggaaaattg atgaactggg tcctgaccga agcgccgtca
     2521 gggacgatct ttggcgtcca ccatcgccta caggccgacc cgaatcggac atatactatc
     2581 tttgacagac cgctggaaat gccctttggc ccggctgccg gtccccatac ccagctggcc
     2641 cagaatatcg ctgccggcta tgcggcggga gcgcgcttct ttgaactgaa gacggtccag
     2701 aaaatcgacg gcgaagacct gcccgttccc aaaccctgca tcaaggccga cgatgaatgt
     2761 tacaatgtcg aatggtcgac ggaactgacc gtccccaagg ccctggaaga atacgtcaag
     2821 gcctggttcc tggtccacgt catggccgtc gaatacggct taggaagtcc ggacgggatg
     2881 cagttcaaca tgtccgtcgg ctacgacctg gcagggatac agacggcaaa aatcgattcg
     2941 ttcatcgaaa atctgaaaga cgcatcccat acggctatct ttgccgagtg caaagcctgg
     3001 ctcctggccc atctggaccg gttccagaaa ctccgtcggg aagacgtcga agccattccg
     3061 gcagaaatct gcaactctgt cacggtctcg accatgcacg gctgtccgcc cgatgaaatc
     3121 gaacggattg cccgctattt actgaaagaa aaacacctca ataccttcgt caaatgcaac
     3181 ccgaccttac tgggctatga cttcgtccgg aaatgcatgg atgacctggg ctatggccat
     3241 atggtcttcg atgacagcca tttcaaggcg gatttacagt accgtgatgc cgtacccatg
     3301 ctcaaggggc tgcaggaagc gggtaaagag gaaggcctgt tgttcggcgt caaactgacc
     3361 aataccttcc ccgtcgacat tacgcgccat gaattgccgg ggacggaaat gtatatgtcc
     3421 ggcaagcccc tctttttcct gtccatgaat gtggccaaga agctgagtga agcctttgac
     3481 ggcagcctgc gtatttctta cagcggcggt gccgatgccc acaacatcaa gaatatcgtc
     3541 cgggccggca tctggccggt cacggtggcg acgacgctgc tcaagcccgg tggctatgaa
     3601 cgctttgccc agctggcaca gtgttttgaa ggtcagcgtg gacaggcgtt tactcgtgta
     3661 aacgtcaagg ccctggatga cttgctggct aaggctcaag atgatgccca ctatgcccgt
     3721 ctggacccga aggcccagca gcgcaagaac gaccaggcac tgcccttgat ggactgcttc
     3781 atggcaccgt gcagccagac ttgccccatc catcaggata tcccggccta tatggacctc
     3841 gtcgccgccg ggaaatatga ccaggccctg cagattattc tggaaaagaa tcccctgccc
     3901 tttacgacgg ggaccgtctg ctaccatacc tgtatgaaca gctgtacgcg caatttctgt
     3961 gatgcccctg ttgaaatccg gcgcaataag ctgatagctg ctgaaaaagg ctatgccagc
     4021 gtcatgacga ctttgcaggc cacgccgtcc aatgggagaa aagcggctgt catcggcgcc
     4081 ggtccggccg gactgtcggc ggcttatttc ctggcccgcg gcggcctgga tgtgaccgtc
     4141 ttcgataaga acgacgaacc tggcggcctg gtgcggacct tcttgtgtga aaagaaaggg
     4201 cagatttcac cagaagccat cgacaaggat gtagccctca tcgaaaagat gggtgtccat
     4261 ctcgtcgtcg gcagggccat cgcctcgctc gacgaattgc agggctatga catcatcctg
     4321 gcggcctgcg gcgtcaaagg gaaaatcggt gacaccctgg ccggcgccat cgaaggcctg
     4381 accgtcatcg gcgatggcca ttacggcaag acgacatccg tcgtcgaatg tattgccgac
     4441 ggcaagcggg cggcggaagg cattttaggc cagatggcca gtgtcgatac cctgattcca
     4501 gctgatgtaa acgacgttta ctcgaagcgg ggtattctgg agacggcacc ggaaacaggc
     4561 tgtgacggcc gctgcctcca ttgtgacagc gtctgcgaag tctgtacgga agtctgcccc
     4621 aatcgggcca atacagccat ccaggtaccg ggccatatgc aggctcagat tctgcatatt
     4681 gactacatgt gtaacgaatg tggcaactgc cggaccttct gtccctgggg cggtgcgccg
     4741 tatgtagata agtttacact cttcgctaat gaagacgatt tagaccacag cgataattcg
     4801 ggctttgccg tcctcgacaa ggccagcggc ttctgcaaag tccgcctgga tggagaaatc
     4861 cggacgacga ctttagggac ggctgatgaa gcaatcccgg aagacctgcg gaccttcatg
     4921 gaaacggtct gccgcgatta cgattacctg ctgattgaat agagaaagaa gggaaaacca
     4981 tggattacgt catcaaaaac gggacggtct gcctggaagg ggaacatttc cgggccgacg
     5041 ttaccgtccg tgagggaaaa atccaggcct tgggacaggc tgtgcacagc ggtcatgaac
     5101 aggtcatcga cgccgacggc ctctatgtct gccctggcgg catcgatgcc cacacccata
     5161 tggacctgca gcagtcgccg cagtaccgcg cgtgcgatga cttttacagc ggtggcgtgg
     5221 cagcggcctg cggcggcacg acgaccatcc tggatcatat ggcctttggc ccggccggct
     5281 gttccctgcg ctatcccttt gaacggtacc ggaaactggc cgtgccctgc ccggtcgact
     5341 acagcttcca tggcgtcatc cagcatgtcg acgaccaggt cctgcaggaa ttgggccagc
     5401 tcatcgacga aggttttccc agtttcaaag cctatacgac gtatggctat cccgtcggcg
     5461 agaagaaact gatgcagctc ctgccggtca tcaagaagca tggcggcctg ctcacggtcc
     5521 atgccgaaag cgacggcatt accaacgccc tgcgggatgc cctgggaccg caggacctgc
     5581 agcccatcgg ccaggcgaag acgcggccca acgtggccga agccgcagcc gtgagccgtg
     5641 tcctggcggc agcccgggct tgtggcgatg cgccggtcta tatcgtccac acgtcggccc
     5701 atgagagcct ggaccagctg cgcctggccc ggcgccaagg ccagcgtcat atttacagtg
     5761 aaacgtgtcc gcaatactta ttgctgacag aagagaaatt ccgtcacggc ggccctatgg
     5821 aaggcattaa atacatgctg gcgccgccgc tgcggaaagc cgaagacaac caggccctgt
     5881 ggcaggccct ggccgacggg acgatccaga ccgtagcgac ggaccactgc ccctttacga
     5941 tagctgaaaa acaggagaat gtcagcgatt tccggaactg tcccggcggc gtatccggcg
     6001 tcgaagaacg gatgcccctg ctgttcagtg aaggcgtcct gaaaggccgg ctgaccctgg
     6061 agcgcttcgt ccaggtgacc agtgccaatg tggccaaaat ctttggcctc tatccgcgca
     6121 aagggacgct gctgcccggc agcgatgccg acatcgtcct catcgatccg aaagggacgc
     6181 ggacctttgc cagggagaac ctgcacacga cctgcggcta ttcgccctat gaaggcatga
     6241 cggtccaggc gacgatccgc ctgaccatgc tccgcggcga aatcatcgcc aaagacaatc
     6301 aattctgtgg aaaacgcggc tatggaacct tgttgtatcg attccgcgat aaaagataat
     6361 cttgattata ggaaggtggc attatgagtg aattatgtaa actatgggta aacggccgtg
     6421 aaatagaagc cgatggagaa cgcaagctca tcgacgtcct gcgcaatgac ctgcgcctga
     6481 aatccgtcaa ggatggctgc agtgaagggg cctgcggaac ctgtacggtc gtcatcgacg
     6541 gcaaggcgac caagacatgt gtccaaaagg tcagccgcat ggctggtaaa cacgtgttga
     6601 ccgtcgaagg gctgtccaag cgggaaaaag acgtcttcgt ctacgccttt ggcgaagccg
     6661 gcgccgtcca gtgcggcttc tgcatccccg gcatggtcat gtgcggcaag gccctcatcg
     6721 acgccaaccc ggcgccgtcg cggctggaaa tcgccgcggc catccgcaat aatatctgcc
     6781 gttgtacggg ttataagaaa atcatcgacg ccatcgctat ggcagcagat ttgctgcgca
     6841 cggaccagcc tcttcccgtc cgcggcggct accgcggcgt cggcgctccc tgccagcgcg
     6901 tcaacgtcaa ggccaaagtc ctgggc
//
LOCUS       DAWABZ010000100         6830 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_2990_length_6830_cov_3.839410, whole genome shotgun sequence.
ACCESSION   DAWABZ010000100 DAWABZ010000000
VERSION     DAWABZ010000100.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6830)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6830)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6830
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2990_length_6830_cov_3.839410"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1595 a         2007 c         1755 g         1473 t
ORIGIN      
        1 cttgcagcct ttcgacatcg tcgtcatccc ggccggcccc atccaccgcc ctatcatcta
       61 tggcgatgat gtctacgaac gcatcatcgc ttatatttcc ccggcttatc tgcaaggcta
      121 tgaacgccgc ggctgcccgg ccggccagat attcacggcg cccaaggctc ccgtcctgcg
      181 ccagccccag gaagcggaca acttgtacaa tgtatcgtgc cgcctgcgcc gggcctggtc
      241 ggacacggga ccggaaagcc cgctcttaca ggaaacgctg tttacggagt tcatgatcca
      301 cctgacccgg gccctgcaga aagacaagct ccgtatcgtc caaaagggcc atcagaacga
      361 gaagatacgc cgcatcctgg cttacatcga agccaacctg agcggcgacc tgtccattcc
      421 cagcctggcc gccgcctttt tcatgagccc ggactacttg atgcacctct tcaaggccga
      481 aacgggcatg accgccgccc gctacattac gacgaaacgg ctgcaaaaag cccgccacct
      541 catgcaggag gggcgggctt tgacggaaat ctgctatgac tgcggattca cgacgtattc
      601 aacattctac agggcctgga aaaaccacta tcacacgtca ccgaaagaag gcatccgcgc
      661 cttccccgat actttcgacg aataatcagc ggttccggtt gtaattgatg aggcagccgg
      721 ctttgacgat tttcttttca tcgtccgtca tgggcgcgat atacagcgac aattcacgca
      781 ggtccttgcc gacgacatag gccttgatgg ctttcatgtc gccgtcgagg gcttttttga
      841 cgtccggcac gtagatgtaa tcaccgattt caaagtccgg ttggccttcc atctggaaag
      901 gcagcatgcc ccagttgatg acattggaac gatagcgctt ggtcgcgtat tcctggcaga
      961 tattggccag gccgccgagg acgcgctggc agctggcagc ctgttcgcgg gccgagccgt
     1021 cgccgggctt gatgcagtag atcatgctgc cgatttccac gtctttcatg gaaacgtcgc
     1081 caaagtgagc gcgcaggacg gcaaagacgt ctttcagagc cgggtcgacg ccagccgggc
     1141 agtcgccggc caggcgggcc cgttcgactt tgtctacggc tttggcccgg cctacgtatt
     1201 ccggatcgcg gcgggacaag gtgaattcag ccaggcccag gggattggag cggtacgacg
     1261 acgtttcgcc agacgggatg agttcgtctg tcgtcgtgac cgggtccatg atcttcgagc
     1321 agaccttgag caaaaccgaa tcggccagag gttccatatc cggccaatcc ttgatgttcg
     1381 gaccatagac cagggaagcg ccttcgtcgg cctggccgaa gccctgatag acgcgggtag
     1441 cgtagggctg gtcgtcgtaa tcatagggcg tttcgacata gtcgtccata tagccttcgg
     1501 ccgacgtcag gacgccgccg ttggctgctg tcgcagcgat ggaacgggca tccatgaggg
     1561 ctaccgaagc gaactggcca ttgcccggct tcgagccttc gcggttcggg aagttacgcg
     1621 tcgtatggcg gatgctgaag gcgttgttgg ccggcgtgtc accggcaccg aagcagggac
     1681 cgcagaaagc cgtcttgaag atggcgcccg cttccaggag cgatgccacg gcgcccttgc
     1741 ggaccaggtc gagatagacc ggctgcgacg acggatagac gtcgagggtg aagttgccgt
     1801 tgccgcagga agcctgcttg agtatatggg cagcggccat gacgttgccg tagttgccgc
     1861 cagcacagcc ggcgatgacg ccctgctgga cctggagctt gccgtttttg attttatcgg
     1921 tcaggctgta tttcaaatcc ttgttgctca tgcggccgtt gatggcttct tccgtttcat
     1981 ggaggatatc ttccaggttg gcattgagtt catcaatggt aaaggcgttg ctgggatgca
     2041 tgggcatggc aatcatcggc ttgatggtgc tcaggtcgat gtagattatg ccgtcgtaat
     2101 aggccacatc ggcgggattc aattctttat aagcatcgcc gcggccatgc tgggtcaaat
     2161 aggccttggt gtcgtcatcg gtccgccata ccgacgacag gcacgtcgtt tccgtcgtca
     2221 tgacgtcgac gctgttgcgg tagtccgtcg tcatggaagc gacgccgggg ccgacgaatt
     2281 ccatgacctt gttcttgacg tagccgttct tgaagacggc accgacgatg gcgatggcaa
     2341 tgtcatgcgg gccgacggcc ggactcggct tgcccgtcag gtagacgccg acgacgccgg
     2401 gataggcgat atcataagta tcgcacaaga gctgtttgac cagttcgccg ccgccttcgc
     2461 cgacggccat agtccctaag gcaccatagc gggtatgact gtcagagccg atgatcatgc
     2521 ggccgcagcc ggccatcttt tcacgcatgt actgatggat gacggcgacg tggggcggta
     2581 cgaagatgcc gccgtatttc ttggctgccg acaagccgaa gacgtggtcg tcttcattga
     2641 tggtgccgcc gacggcgcag agcgaattat gacagttcgt cagcacgtag ggaaggggga
     2701 atttttccat tcccgatgcc ttagccgtct ggatgatgcc gacaaaggta atgtcatggg
     2761 aagccatggc gtcgaacttg attttcaagt gttccatgga acccgacgta ttatgatttt
     2821 ttaagataga ataggcgatg gtccctttcc gcccttcttc cttagaaacg gtcctgcctg
     2881 tgagctgtct gagtttggcc gcatcctgtt ccggcacgat gtccgtcccg ttgacgagat
     2941 atgcgcctcc atcatataac tgaatcattc caagccctcc tttgtcaccg ccagcctggg
     3001 cgatggcttt tctcatcgtg aaaatatcgc aattcaattt cacaatataa aattttaagc
     3061 atttcaataa atattatatc ataattaaaa cggccgtggc tacccttaaa acgttttaca
     3121 ctttacgcga tttttatttt actttctcca taattatgct ataataactt atactttgca
     3181 ggaatatact agaaattatt cccaaaataa aatttatgaa tattcgcgta taaccgatac
     3241 tttataagtt attctcccat attcatactt gcaggaaaga gaggaatcac attggacaaa
     3301 aatatttact tagcccagtt ttacggcaag tcacaaaact acacacagat tccccgcgaa
     3361 tggttctcca agtacggcgt aaagcgcggc ctgcgcaatg aagaccacac tggcgtcctc
     3421 gtcggcctga cccgcattgc cgatgtcgtc ggctaccagc gtgacgaaaa aggccaaaaa
     3481 gtcgattgcg acggcgtctt gtactatcgc ggcatcgaca ttcgcgagct ggccaaagtc
     3541 gaaaattatc aaggttactt atatgaagaa gccagctttc tcctcctctt cggctacttg
     3601 ccgacagaag atgaactgaa tgccttctgc aaggtcttac aaaacggcta cgatctgccc
     3661 gatgattttc tggaaatgaa cctcctgcgt ctgcccggca agaacttgat gaacaaattg
     3721 cagcacgccc tgctgacgct ctacaattac gaccccgacc cggacaatac cgacgtctac
     3781 cagaccctgc gcaagggcct gaacatcatg agcaagctgc cgtccatcgc cgtctatgcc
     3841 tatatgtcca aggtccatta ttataaccgt aagagcctgg tcatccacta tccccgcaag
     3901 gactattcga cggcagagaa tatcctgtcc atgctgcggc cggacagcaa attcacgccc
     3961 aaagaagccc agctcctgga cctgctctta ttcctccatg ccgaccacgg cggcggcacg
     4021 aactcgacgt tcaccaatat cgtcgtctcg tcgacggata cggacatcta ttccagcatg
     4081 gccagctccg tcggcgccct caagggcccg cgccacggcg gtgccaatat ccgcgtatcc
     4141 ctgatgatgg aacaggtcat cgacgccatc ggcatcaagg ctacggatga acagattgtc
     4201 gacgtcatcc accagattct caacaagaaa ttcaaaggct gtcacgacgg cctggtctat
     4261 ggtttcggcc acgccgtcta caccctgtcg gatccgcgcg ctgaaatcat gcgccactac
     4321 tgcggtatcc tggccaagga aaagggcatg accgacgtct tccgcttcta caaccgcttt
     4381 gaaaagctgg ccatgaagac cttgaaagaa gaaaagggtg tcaacatgtg cagcaacgtc
     4441 gattattaca gcggcttcgc ctacaacatg ctgggcatcc cgcgcgacct ctacacgccg
     4501 ctcttcgtca tcagccgtat ggtcggctgg ctggcccaca acatcgaaca caaactgtac
     4561 gacaaccgca tcgtccgtcc ggcagccaag tatgtcggcg atttgatgga ctatattccc
     4621 agaaaggaca gatagatcat gaagacaatc actgtattca aaggcgatgg catcggcccg
     4681 gaaatcaccg acgctgtcct gcagattctc gacgctgcca aagcgcccct ttcctatgaa
     4741 attttcaacg tcggcgctgc cgaatatgaa cgccacggcg ccctcatccc ggacgacgca
     4801 ttcgcttctt ttgaaaagac ccatctcctg ctgaaatcgc cgatcactac gcctatcggc
     4861 aagggcttcc ggtccctcaa cgtcacgttg cgcaacaaat acgacctcta tgccaatatc
     4921 cgtccggcca aatcgaatac ggccgtcaag acgcctttcc ccaacgtcga tatcgttact
     4981 ttccgggaaa acacagaaga tttgtacatc ggcgtcgaat acaagatgaa tgaagatacg
     5041 gtccacgcca tcaagctcat cacccgcaag gccagtgaac gcatcatccg ggccgctttc
     5101 gactacgccg tagcccatgg ccgcaaaaaa gtcacctgcg tccacaaagc caacatcctc
     5161 aagatgagcg acggcttgtt cctagatatt ttccacacca tcgccaagga ctacccgcag
     5221 attgaaagtg acgacaaaat cgtcgacaac acgtgcatgc agatggtcat gcggccggaa
     5281 cagttcgaca tcatcgtcac gcccaacctc tacggcgaca tcctgtccga cctgaccagt
     5341 ggcctcatcg gcggcctggg cctgctgcct tccatgaatg tcggcgacga ctacgccatg
     5401 ttcgaagccg tccacggcag tgctccggat atcgccggca aacacatcgc taacccgaca
     5461 gccctcttgt ggtcagcctg catgctcctg gaatacatcg gcgaaacggc cattgccgcc
     5521 aagatccgca aagccgtcga tgaagtcctg gctgaaaagc atcacctcac accggacctg
     5581 ggcggcagtg ccacgacgga agaataccgc gacgccgtca ttgccaagct gtaagataaa
     5641 aggaggcatt ccatgaagat caccgtctta ggctgcggcc gctggggcag tttccacgcc
     5701 tggtacgccg accacatcgg ccatgacgtc accttatggg gccgtcccgg ttcccagcac
     5761 ttgcaggaac ttatggacac ccgccagaat gaatacctgc gcctgcccga ttctgtgaac
     5821 ctgacttcgg acctgacggc agctctggca gcagccgata tctgcatcat ctccatcagc
     5881 gcccaacagc tgcgcagctt cacgcatcag ctgaaagaag ccggcttcgc cccgtccctg
     5941 cccttcgtcc tggccatgaa gggtctggaa gaaggaagtg gcaagcgatt gacgacggtc
     6001 atggcagaag aactgggacc gtccgtacgc acggccgtct gggtcgggcc cggccacgtc
     6061 caggatttca tcagcggtat gccgaactgc atggtcatcg cctcggctga cgaagacctg
     6121 acccacttct tagtcgatac cttcacgagt cccctcatcc gtttctacta cggccaggac
     6181 ctgttgggca tcgaaatcgg cgcggccgcc aagaacgtca tgggcatcgc tgccggtatg
     6241 ctcgacggcg ctcatctgac cagcctgaaa ggagccctca tggcccgcgg cgcccacgaa
     6301 ctatcggtcc ttgtcgccgc catgggcgga caggccatga ccgtctacgg cctgagccac
     6361 ctcggcgatt acgaagcgac cctcttttcc ctgcacagca ataaccgccg cttcggccag
     6421 gacttcatcg aaggaaagcc cttcaaaaaa ctggccgaag gcgtcagcac gacagaggca
     6481 ctcctcgacc tgtctggaaa atacagcgta gaactgccca ttaccgcagc cgtccacgcc
     6541 atcataaaag aaaaaaagga cccccacgag gtcctgaaaa atctatttct ccgttccact
     6601 aaatgtgaat gataaacttg acgtaattta gcgaaaagga tacaatagac atgaaaaagg
     6661 tgctgtccgt aacgggtcag ccccaaatag cactaaagaa taatcgcctt actgcttgga
     6721 gatgcaaagg cgattatttt ttatttaaaa ccgcaaataa gacaatcaga agtaacaatg
     6781 taatattcag attatccccg tcttccaaaa cttttgattc atttacctca
//
LOCUS       DAWABZ010000101         6619 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_3099_length_6619_cov_3.401889, whole genome shotgun sequence.
ACCESSION   DAWABZ010000101 DAWABZ010000000
VERSION     DAWABZ010000101.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6619)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6619
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3099_length_6619_cov_3.401889"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1422 a         1574 c         2074 g         1549 t
ORIGIN      
        1 ggaagcaact tacttgcgat tcctttcgca tctcttggct atattatagc ataggatttt
       61 acgcttgtca tgaggtatac gaaaatatat tatgttattt gcgcattaaa taatagatgc
      121 gtatagcctt tgggaataat ggggggcgcg gtccgcaggt cgcaggtcgc agtccgcagg
      181 cctcccctga taggggaggt ggcctgaaag gccggagagg ttgcattttc gaacccgctt
      241 gtgaaaatcc cgtaacctgc acggtgattc aaaaagcaat ctcgcagtca gctgcgctga
      301 cagctctcct atgaggagag acttgggata ggacatcaaa aggcgctgtc gcataaagac
      361 agcgcctttt tgagtattaa gtttgtagtt tttagtgaag gcttttaaat ttaaagccct
      421 cttctacaaa ctctgtacta caaactttca ggagaaaggg gcctttcatg atggtaaaag
      481 ccttcgtgcg ccccctacgg gttatggaat taggcgggac gcccaaagga cgtcccctac
      541 gggaccaata atgaggttat ttgtcctgta aaatcggcat gattttggtg gcatggggca
      601 tgatgttgat ggcgtagtcc ttgtctttgc cctgttcttt cagtttctgc tgtgccaggt
      661 cccatgcttc ctggacggtt tcgaccggaa tctggccagt gcggcggatg tcgttgaagt
      721 tacgcggcag tgtgaccagg atgaccgtgt ccttatgagt caggcagaag aggttgaagg
      781 cgacgaagaa ggggatagtg aagtgtgccc gcagggcttt ttccatgtct tccatcgtgt
      841 cgtatttgaa gctgtccatg tagatggccg gttctttgat gtcccgggct tccatgatgg
      901 cgatgatgat gccgccgtct ttgacagcca tttcagccgg atcgtagcat ttgcagccct
      961 ggtagaggct gacgtcgctg ggataaccgc cggcgcaggc gatgacgacg tccgattttt
     1021 ctttcatcgg gacgcgctgg atgtcgagga cggcttccgt gccggcccgc catgcttcgt
     1081 accagtcgcc gccgaccatg cggcaaattt caccgtcgcc gttgatgacg gaatggacga
     1141 ggaagcacgg attgatcttg ctgcaggctt cgaccatgtc atcgttgagc gggttgccgt
     1201 cgatcttgag ggcccgcgtc ttgggattga tgccgtggcc gaattcgtca gctaaagcgt
     1261 ggcaatggtt gcgctggatc gtttcaaaac cggagacgcc cggcaggatg agcttgcggc
     1321 cgccgccgta accggcaaag agatgggtcg taatgccgtc gacgatgata actttgtcgg
     1381 cgtcgacgac gtgtttattg agtttcaatg gtgtgccgag cttggtcgta ccgaagtcga
     1441 cgagagtcgg atccatgcag tcgtgctgat aggtcttgat gcggcggacg acttcttcgc
     1501 cgcagacgag gacgtcttct tccggcgtct gggcgcggtg cgagccctgg gcaaagacga
     1561 tgtagatgtc gtcatcgggg atgccggcca ggttcagttc gtcgatgata tagataagga
     1621 attgattgga atggttccac gtgcgggtga cgtcggcgac gacgaggcag accttatcgc
     1681 ctttctggac tttttcctgc aacggggcgc tgccgatagg atgacgcatg cagtcgatcg
     1741 tcgcctgttt gacgtcgacg gcagggacgt gtttcccttc catgaccccg acaatgtgtt
     1801 cttccgggag cttgacagtc tgcgtagttt caccaatatt aaaagtaaat tcctttaatg
     1861 ccattcttta ccaccacttc tttttaaaaa ttaaaaataa actgtaactt attatatcat
     1921 gttcaaaaca gtgcgcaagt ttaaacttta cgataatcgt ttaacgtaca agggatagca
     1981 tcagctagac attgcctaga tagacgtagt gaagataagg aatggccgcg gcctgcagct
     2041 tcttcagcgt ttctacaggt gtcaccgggg cagaccagtg gtactgcggg aaatagcggg
     2101 tcagatgcag ggggatgtcc ggtaaaatgg atgccagcca gcgggcttcg gcgtccatct
     2161 gggccgggtc atcgttttcg ccgggaatga ccagcgtcgt cacttcgaca tggcttacgg
     2221 cagcggccgc ttcgatcgtg tccagcacag gctggagaga tgctccgcag agggactggt
     2281 agaagtcctg ggaaaatccc ttgaggtcga tgttccaggc gtcgatatag ggaagcaggg
     2341 cctggagtgg ttctttttcg atatagccat tgctgaccat gacgtttacc aggccggctt
     2401 tgtggacgag gcgggccgtc tggatgatga attcatagct caaggtcggt tcgttgtagg
     2461 tgtaggccag accgatgctg ccgtcggccc tataatgccg ggccagggcc agggcctgct
     2521 ccggtgtcag ttcccgaaag ggcgcttcct gctgggaaat ctgccagttc tgacagaagg
     2581 ggcaggtgaa attgcagccc cacgaaccca gggacaagat ggcagaaccg ggatggaagc
     2641 gatagagggg cttcttttcg atagggtcca gggcgatggc cgtgcattgg ccgtagttca
     2701 gggcctggag gttcctgcca tcgtaatagc gggtctggca gcggccgcga tggccctggg
     2761 cgatgtggca gtgatgggga cagagacggc agcgagcctg gccggacggg tcgatttcat
     2821 aataagcggc ttccatggtc tcacctactt atagcgcgtg acggtgaagc ggtagaggtc
     2881 aggctggacc tgaggcggga tgccggcttt ttccttggca atggcgattt gctgctggac
     2941 cgtgtcgacg ccttccaggt cgggcagtaa gaggcccgtc tgggcatggc tcatgacgat
     3001 gacgccgtac ttcttggaat ccaggtcggc tggtgaagct acggcttcgg gctggcccag
     3061 gacgtcgacg gaaatatcga tgtctttcag ttcgtccagg ctgaccgggt agaagcgcgg
     3121 gtcccgcgtc gcagccgata cggcgttgtg gatgatttca ctggcaatat tcatctgcat
     3181 gggcaggaag gtgccgatgc agccgcgcag gcggcttccc ttgtggaggg acacgaaggc
     3241 gccggccttg tcctgcaggt cttcaggcag gtatttgggt acgttcatga aggcatggtg
     3301 cgtcaggtag tacgagatgc tttcccgggc caggcggacg cggatgtcgg cgacagccgg
     3361 tttttcagcc cgtttttcct ggccttccgg caggtacagg gcgacgccat agccgacgcc
     3421 gaagggccct tcataggaat aaatcggcat gaccgggcgg aaatggcgca gggcgccgaa
     3481 gaggaaatac accgacggca ggccgcacat gccggcttcg tcgatgaggc tgcggctcat
     3541 agtctttaag atggccgggt cctgtttttg cagggcctcc atgacggtct tgtcgaagat
     3601 ggcccctttc ggcgtgtagc cgttcggcga accgggcagg agacggtgcg acaagtcgcc
     3661 cgatgcgatg atggcgatgc gccggcccag tcgggcagcg gcctggacga cgagatcgcc
     3721 taagaggctc atgctgtcat agtcgtcgaa gcgcggcgcc atgaggacga cctggcccgt
     3781 gaagccggcc tggcgaaggt aatataaggg gacgaaagtg ccctggtcga cgtagagggg
     3841 atggccgaag cggtcgctcc aggcggcatc gagtcggtat acgtcggtct tgggcgtggc
     3901 tgtttcaaag atttcttcgg ccaggtccat atcggtgcgg acatccatgg ccagctccgg
     3961 atagccgaaa gcgtcgaggt tgccgtagag gcggggcgtt tccacgagcg ttgccccgtc
     4021 ggggaagacg tagttatgag gcgacatgat gaccaatgtt tccggtttct ggctgatgat
     4081 caagcgggca gccgcctgta cggcccggac cgtggcgtct atctttcgca gttcatcgcc
     4141 gccgatttcc ggcagcataa tcggcggatg gggaaataac cccgctgcga cacaagcatg
     4201 ttccataaaa aaacctccct cataatggta tatgtctatt ataccattat gagggaggtt
     4261 tgactaaagg gcttgtcgca cgacgacaag ccctttaaag tggttagcgg ctagcagtta
     4321 gttgttagca gatggcttta aatttaaagc tatcgcttca aacatcatac atcaaacatt
     4381 aaagagaaag gaatcatgca tgatggcaga aaccttcgta cgacagcggc ctgtgaaagc
     4441 ggttcagggg attcccgcaa gcgggacgcc ctttgcggcc tgcgaactgc gggccgcctt
     4501 aacaggccgg cccctactag tattccgtgt cttccatgag gctggtcttg ccgaagcggg
     4561 tcttcaggtc tttccgttcc atgctccatt tggacgaggc gatggacatg gtcgggtcgt
     4621 agttcggcga ttccatgttg aggacgccgc agatgaattc gtattccagg tcgttggtga
     4681 aatacttgtc cttattggcc ttgatggctt tgacgacgtc gggattgcgc ttctggtagc
     4741 cttcagacaa gtagcagaac atggggatgc gcaggacttt gaagctggca ctgtctggct
     4801 ggcgggcgac gtcgggatcg ctgccgtgat cggagaagta gatcatggcg tcgaggttga
     4861 ggttatcccg ggcgtagtcg taaatctgcg acaggaccca gtccgtatag aggacggtgt
     4921 tgtcgaagtc ggcttcctgg ttaatcttgc cgtcgttgaa tttctggaac tctttcgggt
     4981 agcggttgcg gtattcgatg tggctgccca tgacgtggag gacgacgaag ttcttttcct
     5041 tcggatcgac gcgtttgagg aactgcagga gcgcgccgtc cagggtcgat tctttcaggt
     5101 cccggtcgac ccattcggag acgtcggctg tgttggcaac cagcgtaatc ggtgtatcgg
     5161 cgacgccgac ggagccctgg ttgctgaacc agtaggtctt atagccggcc ttcttggcca
     5221 tgtcgatgat ggaaatggat tcgttgaaag gcttatcgtt gtagaaattc gattccgtca
     5281 gggcgtgttc caggacgggg accgtgtacc agacgcagct gtaggcgttg gtgaacagcg
     5341 tgaagtccgg attcgatttc atcgccgtca gccacggtgt attgggcacg tggtccggat
     5401 tgtaggcatt catcagcgtc cgcgtttccg attcgccgat gacgacgatg accgtgttgg
     5461 gatagtcggc cggatggagc tggacggcct ggagggcatt gtatttttcg tcgtggtttt
     5521 cggcatataa agccgagcgc tgcatgtagt cgtgcgtgtc gatgaaggtc cggatcggga
     5581 aggcttccgg gaagatttct tcgctcaagg cgccgacaga cggggcgatg atgagcagca
     5641 gcgaagccag ggggatgatc ttgcggtaga cgtgtgtccg gggcaggatg ttctggcgat
     5701 agttcaagag gaacagggct cccagaatca gggccagggc gacggcgatg gcggctacct
     5761 tgaacaggcc cagggagtgg aagtattcca gcgtttctgc cggtcccgtc tggaagatga
     5821 gcagggcgcc ggacgtggtg atcgacgaat tgtagaggaa atagtagacc cactgcagca
     5881 aaatgatgaa gaacgctgcg aactggacga gggtcatgaa gacggccccg attttgtggg
     5941 ggagatactg ccggacaatc atgttcaggc agaaaaagat agcaaaaaag tatagtccta
     6001 catatatatc atatatattg ttcaagtaaa tcgtattcgt attgatcagg gaaatgtaac
     6061 tcaagtaagg gcccgtaatg atccagccca ggccgatgag ggccggcggg atgatggcgc
     6121 ctggtgacaa gtgcttccag tacagggcca gggacaggac gacagcaccg acgccgggca
     6181 gcaggccgaa cgtcatgtct tcgatattgt cgggacccat gcccagggag acgagaaact
     6241 gccattcaaa gcagaagccg tagactacgg cgaagatgat aagcagcggc aggagccgat
     6301 gacgacattt gctgattgcc ttcgttgcat gaaccatgtt gtttgttcta tcctctctta
     6361 atctctttgt aaaaacggtg taaacaaagc ataccggcta tattatacat caaatgtagc
     6421 cgataacata gagggatttt cggaattatt ggaaaaaaag ataaaaaaag acttgccaaa
     6481 aatgccgtgt catggtagaa tagttttcgt tgaggcactc cgtgctgata caatggtgaa
     6541 acggccccgt ggtgtagcgg tttaacatgt cgccctgtca cggcgaagat cgtcagttca
     6601 aatctgatcg gggtcgcca
//
LOCUS       DAWABZ010000102         6496 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_3167_length_6496_cov_3.253532, whole genome shotgun sequence.
ACCESSION   DAWABZ010000102 DAWABZ010000000
VERSION     DAWABZ010000102.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6496)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6496)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6496
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3167_length_6496_cov_3.253532"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1459 a         1807 c         1934 g         1296 t
ORIGIN      
        1 ctttgggcgt cccctacgta gccccagtcg gggcattttt aacatagaaa aaggctgtca
       61 cagagtgata gcctttttct gtattggtat tgggttggaa agcggattaa tggttagccg
      121 gatggccttc gtggacgcct tgttcagccg gtgcagcagt acccggaatg ggagcggcac
      181 cgagttgtcc gcggcgtgcc tgggagatga tgctgtggtt gtagccatag ccgaaataga
      241 tggccaggcc taacagaagc catacgacga agcggatctg cgtaaccagg ggcagcatgt
      301 agatgaggac accgcagaag gcgatagcca ggagcggaac caggggaacg agcggacatt
      361 tgaaggcgcg tacctggttc gggttcttct tgcgaaggac gatgacagca gccgaaacga
      421 tgatgaaggc acagagtgta ccgatattgg tcatttcagc gacgatgttg attggcagga
      481 accctgccgt aatagccgta accacgcaga ccagcatgga gctgcgggcc ggagtcttat
      541 atttcggatg gacgtggccg aagaatttcg gcagcaggcc atcgcgggac atgacgaaga
      601 gtacgcggct ctggccaaag ctcatgacca ggaggaccga agtcaggccg cagatggcgc
      661 cgacagagat agccgcagca ccccagtgat agccgacggc ctggagggca aaggcaaccg
      721 gagctgctgt cgtcttgaat tcgaggtacg gaaccatgcc ggtgaggacg gccgatacgg
      781 cgatgtacag gaccgtacag atgatgagcg ataagataat gccgcggggc agatctttct
      841 gcggattttt gacttcttca gctgctgtgg aaacggcatc gaagccgata taagcgaaga
      901 agatgatgga cgcgccggcg aagacacccg accagccata gggcatgaag ggaacccagt
      961 tggcagcatc gacgtggctg aagcctaaag ccaggaacaa ggcgacgacg gccaatttga
     1021 tgccgacgat gatgttgttg acagttgcac tctggcggac gccgtggatg ttgatgaagg
     1081 cgatgacgca gaggatgagg acggccggca gattgacgat gccgccgtta tatggagcca
     1141 gtgtcaaggc tttcggcagg acgaggccca agttggtgag gatattattg aagtacccgg
     1201 accagccaat agcgacggcc gatacggcaa aggcgtactc tagtatcaag tcccagccga
     1261 tgacccaggc ccaaaattca ccgagggcga cgtagccgta agtataggca ctgccggcaa
     1321 cgggaaccat ggcggcaaat tcagcataac acagggcggc acagccgcaa gccaaagctg
     1381 cgatgatgaa agaaatgata agtgcaggac cggaataatt ggcagcggca acgccggtca
     1441 gaacaaagat acctgtaccg acgatagcgc cgataccgag catggtcagg ccaaaagtac
     1501 cgaggacgcg gttcatgccg ccgtcttgtt tcgtttccga aataaattca ctgatacttt
     1561 tggttctaaa gatgtgtttc gtcatagaac ctctcccctt atgattgtaa agtatcgttg
     1621 actcccctat ggattgaccg gatagtcaat cgttcaactg ccttttatta tactcgtaca
     1681 ttttaatttg taaatgtttt atcctaaatt aattatattt ttatatttaa aattaaaaat
     1741 agcaaaaata tgttcaatca aaaagttgag gtaaactata atattaatac gcttaaatca
     1801 taatctattt atccagtgtt ttatttaaaa agattaaatc atcctggtaa tctatatcaa
     1861 ataattcacc ttttcttttg ggataataga cccaggtctg aggacgcttc agaaactgcc
     1921 ggcctcccgt atcgccgcgc agggtcagga gtcccggtcc ataagggcgg cggaaaacgg
     1981 cagggctgca gcgccgctcg ccgttatgca tacagcctac agctgcgcca gaggccagga
     2041 actgccgcag gaagtcggca atcgtttctg ccgtcagcaa gggctggtct gccggaaaaa
     2101 aagcccaggc gtcggcttca ctatgagcct ggacggcgca gcgcaaagac gcagccatac
     2161 cttctcccgc ctgaccgttc catatgacgt caatgccctg agcctggcag gctgaggcaa
     2221 tggcatcatg agccgtcacg accgtcacct gacagtcgat accggaagat ggcaaaaaag
     2281 cctgtgccgc ctggcacagg cttttcattc catacgtata taaagggcgt ccatgaacga
     2341 gcacttccaa tttatggctg ccaaaacggc ggccgaagcc ggacgccatc aagactaact
     2401 gtattttcat ttcgcttccc tcatttccac tggatacgtc gtcactattc ctggccggga
     2461 caggccgctc aaaatccgtt tccccgcctg ctgccgccgt tcatcgtcgg cctggttgag
     2521 gacgacggtc gtcctgtctt tccaggaccc gatgcgggcc aggcagccgg cttctacaat
     2581 ataggccagg aggcgttcgg tcacgacggc ttccggattg agatgcaata actcccagcg
     2641 gaaacagacg tccttcacga tccgtcccac ggccgacagg ccggccaggc agtatacatc
     2701 acggctgttg gccgggatga ccggttcatg ggggcccatg accttcaagg gcaggcgccg
     2761 tgagccatcg ccttcgacga ggacgacgtc ggcagccgca cataaacgca ggtaaacatc
     2821 ctcgccgggc caggtgattt tcccgttcag cccttcctgg ccgacgacga tgatgccgtc
     2881 gcgccgccat tggccctgcc agcctgccgg caaagcggcc ggtacgaacc atcggctggg
     2941 gtgcttcata tgggtcgtcg tcacgacggc cacgcggcgc ccggcagccc gttcctgccg
     3001 ggcccgggca aagatactgg ttgtcttgcc gccggcaccg atataggtgt atacctcggc
     3061 cgtcatggtt tacggtagcg gctcttttcc cgcccttccc gcttggcccg gacggcaatg
     3121 agttcggcag cgatgctgat ggcgatttct gccggcgtca cggccgaaac ggccaggccg
     3181 atgggcgcgt ggcaggcatc gatttcggca gctgaaaaac cgtcggccag gagctgccga
     3241 tgacgccgag gtaatacggc ttgatggcca gcatctggcg ctggacatcg tagtcgccga
     3301 cgtggccacg ggtcatgatg cagacatagt catcctgcgt tacgccgagg tccgccggaa
     3361 gggcttcata atcagcttta tagacggcat ctgcctgggg gaacagagcc ggatcggcaa
     3421 attccgcccg gtcatcgacg acgatgcagg aaaagccgat actggccagg accgggacca
     3481 gggcctgaga cacgtggccg ccgccaaaga cgaagacccg tcccggataa gtcaggggtt
     3541 cgctgtacca gcgccgctgg ccatccgcag caaggccggc tacattctga cggcaccaag
     3601 ccgcatcgac ggcctgcaaa gccgccatct ggccgcagcc ctgcacctgg ccttcccgga
     3661 ccagggccat gccccaatgg gcttcatcgc tgacatccag ggccagccac gacgccgtct
     3721 tctggcgcag ggcctggatg atagcggcca gtacatccag caggcccggc agggccgggt
     3781 cgaggtactg gcagaagacg gtcaattccc cgccacaggc agcgttgata tcttcttcgt
     3841 cattggggtg gaggatgagc cggcgaaaag ccgactggcg gctctccagg cattgccgtc
     3901 cggcctggat ggccagatat tcctgataac cgccaccgac agtaccggcc gtcgtcccat
     3961 cggcaaagac ggcctgatgg ctgccgatgc cgcggggcgt cgagccccgg ctggcgacga
     4021 cggtcaggag gacagccggc tggccagact gcaaagtttc atataatcgg gtatagaaag
     4081 cttccatgat atcacctata taagagtaaa atagcctgcc catcgacagt gaggcagtcc
     4141 ggcaggacag ccggccggtc gggcttggcg aaacgccacc agatatccgg cgtcccgcct
     4201 tcctgatggg catagcggaa cttgacgatc cattcaaagg gctgctcgat ttcctcggtc
     4261 acgacgatgg gcctggcatt ttcgccgtcg ccaccgcaaa aggcgtggcc gcggatgccg
     4321 atggcacaga ggtcgtcgcc gacaggccgt cccgtctgca gcgtaatctg ccacgacggg
     4381 atgaagacgg ctgtcggtcc ggccttgcgg gccgctacga tattcttgca gcccgtcagg
     4441 accgctgcca cccgggactg gggatcggca aagagttcct ttgtcggcgc acaggccgac
     4501 acgcggccgt cgtctaggac ggcgatggac tgacagagtt gataggcttc gtcgcggctg
     4561 tgtgtcacca agacgacgtc tttcgggaat tgacgcaaca agtccttgag ttctgtcatg
     4621 accttttctt ttaaataact gtccagagcg ctgaagggtt cgtccaggag caggatgtcc
     4681 ggctcattga ccaggatgcg ggccagggcc acgcgctgct gctggccgcc ggacaattcg
     4741 gccggtttct gtttctccag gcctgtcaaa ttcaggcgtt ccatcatgga acggacctgc
     4801 cggcgggcgt cagccttgga cgaagccgag cggacgccgc agcggatgtt gtcttcgacg
     4861 gtcatgttgg ggaacagggc atagtgctgg aacagatagc ctacgcgccg ctgcgggacg
     4921 gggacattga tatggcgccc gctgtcgaac aaggtccgcg ccccgacgac gactttgcct
     4981 gcatcaggct tttcaatacc ggcaatgcac tgcagagtct tgctcttgcc acagcccgaa
     5041 gcgccgagca ggccgaggat gccgctctca tgatggagct tcacatccag cgtaaaagag
     5101 ccccagtcct tatggacatc gatatcgatc atgccaggcg cctcctttca cggctgcgct
     5161 tttcaaagag attgactgtc agcaggacga cggcgctgat ggcgatgttg accaggaccc
     5221 agatgaaggc gctgtattcg tcattggtcc gccacaactg gtagaccgtc gtcgaaatgg
     5281 tcgccgtatt cttggggata tagccgatga gcatactcgt cgccccgtat tcgcccaggg
     5341 cccgggcaaa ggccaggacc gtcccggcga tgatgccctg cttgcagtaa ggcatgcgga
     5401 tatgccagaa gatgaacgta tccgacaggc ccagggtctt gccactccag gccagggttt
     5461 cgtcgaaact ctcaaaggcc ccgcgggccg tgcggtacat gaggggaaaa gcgacgacag
     5521 ccgacgccag gacgccgcca taccaggtca tgacgaagcg gatgcccacg tcattgagaa
     5581 agatgccgac agggtgcttc aagccgaaga ccaggagcag cagccagccg cagacagtcg
     5641 gcggcaggac caggggcagt gtcaggacga catcgaggag ccctttgagg acccgcggca
     5701 ggcgggcgac ataataagcc agggcaatgc cgctaaagaa gacgaggaca caggaaatag
     5761 cggcaatgcg caggctgttc caaaggggat accagtccat aagcagctat ccttagccga
     5821 cagggctgaa gccgacgccc tggaaaatct tcatggcttc ttggcccttg aggtaattga
     5881 ggaaatcctg agcttcctgc gggtgcttgc tgttcttcat gacagccgcc ggataaatga
     5941 cctggccgca catatctttc gtctcttcgt cgacaggttt caacttggca ctgaaggcat
     6001 cggtcgcata gatgacgccg cagtcggcac tgccctcgcg gacctgggtc gtcacttctt
     6061 tgacgttcga gccataggta atgtggccgg cctgagcgat ggcctgttca tcgaggttgt
     6121 agaatttcag gattttctgt gtatactggc cgacaggaac gtcgctgttg cccatgacca
     6181 ggcggatgga gccatccttc aatttcgccg ccatatcatt gaaattctgg acgcccttgg
     6241 ggttgccttc gggcgtcacc aggacaacct tgttttccaa caggtctatg cggctgccgc
     6301 tcttgacgaa gtccagctta tccggattgg ctttggcgtc ttttgaggcg tcgagctggt
     6361 tcatctgctt ctgggctgcc gaaatgaaga ggtcacagtc ggcaccgttc tggatctgcg
     6421 tcttcagcgt gcccgacgaa tcgaagttaa aggaaatttc cacgtccgga tgttctttct
     6481 gatactgggc ggcaat
//
LOCUS       DAWABZ010000103         6487 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_3171_length_6487_cov_3.487407, whole genome shotgun sequence.
ACCESSION   DAWABZ010000103 DAWABZ010000000
VERSION     DAWABZ010000103.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6487)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6487)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6487
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3171_length_6487_cov_3.487407"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1450 a         1716 c         1744 g         1577 t
ORIGIN      
        1 gcttggatct gctttggacc gagacagaaa ggatggctca tcatttgttg gtagtgctga
       61 aatacgtggg atttttcaca atttcgcctg ccagtatgga cgtttgtcgc ttagacacct
      121 ttcggtctgt gccggaaggt gttttttttg tttcttttgc cccagattct catagggagg
      181 tttttttatt gaaaaagtgt tgtgattttc tggtaaacaa catggccatt ttcgtcgtcc
      241 tcatcggtat tgtcggcgcc gtatggcccc agaccttgca atgggtcggc ccgtacgttg
      301 gctggctgct ggggattgtc atgttcggca tgggcatgac cctgactgta caggatttca
      361 agaccgtctt caagcggccg tgggaagtcc tcatcggcgt cgtcgctcag ttcctcatca
      421 tgcccttcgt ggcctgggcc ctggtacgcc tctttgccct gccgccggaa ctggccattg
      481 gcgtcatcct cgtcgggacc tgcccgggcg ggacggcatc gaatgtcatc tcttatctgg
      541 ccaaaggcga cgtcgccctg tctgtctcga tgaccatgtc gacgacgctc ctggcgccta
      601 tcgtgacgcc ggccctgacc tggttcctgg caggcgcctg gatccatgtt tccttcgtat
      661 ccatgatgat ttccattgcg gaaatggtcc tggtaccggt cctgctcggt ttggcggctc
      721 atcatttctt cgcccgcggc gtcgaaaaag tcctgccggc catgcccgtc gtatcggtag
      781 cgactatcgt catcctcgtc ggctgcgttg tatccctcag tgcatcgcgt ctctttgacg
      841 tcggcctgct catggcggct atcgtcatct tgcacaatgt ctttggcctg gcccttggct
      901 atggcatggc caaattgttc cgcctcgatt cgcgtaaatc ccggaccgta gccatcgaag
      961 tgggcatgca gaattccggc atggctgcgt ctttggccgt cttatatttc aacccggcag
     1021 cggccattcc cggcgctatt ttcagcgtat ggcacaatat ttccgggtcc atcgtggcca
     1081 atttcttctc gcgccgcgat gaaaaagccg aagaaaaggc tcgtgccctg tctgtcgata
     1141 aatcgagtgt tcctactcat taattcatat ttcaaaggag tcttacaatc atgaaaattt
     1201 tccgtactgt caaagaaatg caggcatttg ctggtgaaca gaaaaaggct ggtaaggtta
     1261 tcggcctggt cccgactatg ggtgcgctcc acgaaggtca tctgaccctg atgcgggctg
     1321 ccaaggccaa atgcgacgtc gtcatcgctt cggtcttcgt caatccgacc cagttcggcc
     1381 cgaacgaaga ctatgatgct tatccgcgtc gcttcgatga agactgccag aaattggaaa
     1441 gcgtcggcgt cgatgccgtt ttccatccgg aaccgtcgga aatgtatcct gaaggctact
     1501 gcacctatgt caatgtcgac ggcgacatta cgcataaact ctgcggggct cagcggccta
     1561 tccatttccg cggcgtcgct acggtcgtca cgaaattgat gaacattacc cgcgccgatg
     1621 aagccttctt cggtcagaaa gacgcccagc aggtcgtcgt cgtccgccgt ttcgtcagcg
     1681 acctcaatat cccggtccac atcaatatgg tccccatcgt ccgcgaagaa agcggtctgg
     1741 cccggagctc gcgcaatgct tacctttcgc cggaagaaaa acaggcggcc ctggttctgt
     1801 cccgcagcct gcgcaaagcc aaagccgctt atgaaggcgg cgaaaaggat gtagaaacac
     1861 tgaaatccat cgtgaccaag gaaatccagt cggaaccgat ggcttctatc gactacgtcg
     1921 atctcttctc cttcccggcc ttgaaaccga ttgcgacggt cgaccaggaa agcctgttgg
     1981 ctatcgccgt ccgcatcggc aagacgcgtc tcatcgataa cgtcatcttg ggctaaggaa
     2041 atcttcacat tatcttcaca tttcctccac atctttttcg cacccctctg gtatagtaga
     2101 agcataaaca gaggggtact acaaaggagg ttttcgtgat gaagaaaaaa atcttagccg
     2161 gattggctgc actcgtagtg attgccggtt tttccgcctt tgcagcgaca ccagctgctt
     2221 cagatggttc atcgtcaggg acttacggcc cgggctatac gtgttatgac aacggtaacg
     2281 ggtattgccg gggaccgcgt gcctgctatg atgaccagga taactgctat actggaaatg
     2341 gctattgcca gggaccgcgc cgcggctgcc gggctgatgg ccagcgcggt tgtcagggcc
     2401 gtcaggattg ccctgcctat agcgctcaac agtaagccgt tctaatcaaa gggggaaaac
     2461 aatatggtaa tgactcacta catggaactc ttatcgctct atgaaccttg gtttttgatt
     2521 ctcttcatgc tcgtaccgat ggtactggcc gaaacgattt tggctgccgg tgctttttcc
     2581 ttgctctaca aggacagccg cagtgaaaaa tgggacagcc tgagccacgt ctgcggcctc
     2641 atcctgggtg tcttcttcat cgtagctaca gtatacatcg tcacatccta cgtgccgact
     2701 atccagtggc gcggcccgat tgattacatt tctatctggg cctatgtcct gggcgtcatc
     2761 ccggccgtcc tcatcctctt gcaggaattg ggcatcatct ttaagggcct cgatacaacg
     2821 gcaaaaatca agaaacacat cgtcctcatg attctcttcg tcttgttcac ccatttagcc
     2881 atggtcttcg gcatggccga cccgcagctc gcaggctacg tcccgccgaa acagaacatg
     2941 cagatgcaga tgaacggcaa tatgcctatg gaccacagtc agatggacca tagccagatg
     3001 aatcacgacc agatgaacgg tcaaatgaat ggtcagatga ataacaataa cggccagatg
     3061 gaccacagca agatgtagtt tcgtggctag tggctagtgg ttcgtggctt cgtaggggcc
     3121 gtcccacagg gcggcccgcc tctcctgtta ggagaggtgg cccgaagggc cggagaggtt
     3181 taacctctca gtcagctgta gctgacagct cccctataag gggagccatt atacgagcca
     3241 cgaaccacta aaaagggctt gtcgcacgtg cggcaagccc ttttttcatg ctataattga
     3301 tgtaaaaagg ggtgaacacg atggacttag cgcagccgat tttagagggc atcatcgctc
     3361 tggctaaaca atacgatatt caaaaagtca ttctttttgg ctcccgtgcc cgtggcgata
     3421 attgggaacg cagtgatatt gacctggctg tctgcggtgg aaatgtcacg cggttttctc
     3481 tcgatgtcga cgaggtcata ccgacgctgc tcatgttcga tgtcgtcgat ttagatgaac
     3541 cggtccagcc cgaactacga gccgctatcc aacgggaagg agttgttata tatgaaaaag
     3601 tatgagaatt tctgcaaagc cttgaataac ctcaaagaaa taccgcctta ttcgacggtc
     3661 gtccaaacgg gaatggtttc cctttatgaa atctgctttg aacaggctgg gaaggccatg
     3721 aaagaagtat tggaaaacaa tggctatagc cagcataaga tagggtcacc gcgggcgatt
     3781 atcaaaatgg cttatcaggc caatctgatt gacgatgagg attgctggct ggaggcgttg
     3841 cgggaccgga acaatgtagc ccattcttat aatgaagaaa ttgccaataa tatcatcaag
     3901 gacagcaagg aaaagtacat cgccatgttt gaagagttgc agcgggaaat agaaaaaaat
     3961 tggttgtgaa aatgatcggg aggtggaatt ttgcgaacga attcgatttt gatcgtcgat
     4021 gatgatgaaa aactctgtgc cctgctgcgg tcctatttcg agcaggagcg gttcatggtc
     4081 tatgtcgccc atgatggcat gacggcgtta catacactgc gcacccagaa gccgcagatc
     4141 atgctcctcg atttgatgat gcccggcatg gatggcttcg aagtctgccg ccgcatccgc
     4201 cagttcagcg atatacccat catttttctg tctgccaagg atgatgaaac ggaccgcctg
     4261 gtcggcctca atatgggcgg tgacgattac gtgaccaagc ccttccacgt caaggaagtc
     4321 attgcccgcg tctacagcct gttgcggcgg acccgtggtg aagtactgca gcagacgacg
     4381 tcgtatacca tacgggacct gactatcgac aaggaaaaat ttaccgtcct gcggggacag
     4441 cagcccattg ccctgacgcc gacggaattc aatctcctgc tggttctggc ctcggcaccg
     4501 gatcgggtat tcagccgcat gcagctcgtg gataaagtac agggcggcta tggctttgaa
     4561 ggctatgaac ggactatcga cacgcatatc cgcaatctgc gcaagaaact ggagcccgac
     4621 ccggcccatc cgacgtatat cctgaccgta tacggtatcg gttataaatt cgacggaggg
     4681 aatcatgaag aaagctgaca gcatcgtcat ccgcctgacc gggatggtct ttatcctgat
     4741 tttcctgacc atcgccatcc tgctcatcct ggtcaataat cagatggaca gccatttttc
     4801 ccagtatttg aataacatgt cgcaaatgat gggaaacagc catcagggca tggggatgat
     4861 gcacggcatg atgcatgggc cggcggaaat tacgtatata tcggctgtcc accggtcgct
     4921 gttgtgggtc ggcctggtca tgctggccgt cagcgtcacg atcagctact tcattgtccg
     4981 ggaaatcatg cggcccttgt cggccctgac cgaggctgtc cagaaggtca aggccgggac
     5041 gtatggccag accgtgcccg tcgaacgcca cgacgaggtc gggaccctga cggagacgtt
     5101 caacgacatg tcccaggaac tggctaaaaa cgataagatg cggcgccgcc tgtttgccaa
     5161 tatcgcccat gaactgcgga ctcccctggc tatcctccag ggcaatctgg aaggcatgat
     5221 cgacgatgtc atcccgacgg ataagaaggt cctcctgtcc atggaagatg aaacactgcg
     5281 catgggacgg ctcatccagg acctgcgtga cttatcgctg gcagaaatca atgaactgac
     5341 gctccataag gagccgtatg atatcaacgt cctcctggaa cgggccgtga gcatgctcca
     5401 gcctttatgc gacgaaaaat ccctgaccgt ccatttggac ttggccaggg atttaccgaa
     5461 agtatccatc gatatagacc gtatcaatca ggtcatttat aatctgctga acaacgccat
     5521 ccgctatatc gaagcgggat gtatgattac cgtttcgacg caggtcgtca ccgtacaggg
     5581 ccagccctat gtgcaggtcc agatcgccga tacgggcagc ggcattgccc cagaggattt
     5641 acagcatatc ttccagtatt tctaccgtag cgaaaaatcg cggagccgca agagcggcgg
     5701 cagtggtatc ggcctggctc tagcccggca gttcgtccgc agtcatggcg gcaggatatg
     5761 ggcggacagc gaagtcggca gagggacgac gtttactttt attttaccaa ttcatagcca
     5821 gcatcggcaa tgactttggc aaaggcttcc gtcgggatgt ctttgctggt cgtgacgtcg
     5881 gcttttttac cttccaagtc gacagtgaca gccgtgacgc cgtccatggc agccaatgca
     5941 tcgtgtacgt gtttctggca gtgctggcac atcatgcctt cgattttcaa tgttttttcc
     6001 atcgtttcat cctcttcttt ctttggtgta ctgagatttt gtaatgtata tccagcagta
     6061 gtgatgactt ttttgaagtc ctctgcggag atagctttgt cagccgagac atcggctttt
     6121 tgaccatcga ggtcgacgac ggcatcggtg acgccgtcca tggctttcag gactttcgtg
     6181 accgtagcca cgcagtgctg gcacatcatg ccttcgatgg acagggtata gtgatacgga
     6241 gccggagcgg ctgccacttc gggaaccggc gtttgcggcg ctgcctgtgc agctttcggg
     6301 ctactggttt ccggcgccga tgtttcacgt gaaacatcgg cactgtgcgt cgtcttgaag
     6361 aagcgcaggc gcagggcgtt catgcagacc gtgaagctcg acagactcat ggcagcggcg
     6421 ccgatcatgg gattgaggcg gatgccaaag gccggataga ggacgcctgc agccaggggg
     6481 atgcaga
//
LOCUS       DAWABZ010000104         6394 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_3235_length_6394_cov_2.679602, whole genome shotgun sequence.
ACCESSION   DAWABZ010000104 DAWABZ010000000
VERSION     DAWABZ010000104.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6394)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6394)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6394
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3235_length_6394_cov_2.679602"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3445..3483
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1343 a         1832 c         1675 g         1505 t
ORIGIN      
        1 tccccaggtc ccgccaatgg tgcacttctt cttccaggag gttgaactgc ttctggaaac
       61 tggccatcat cttggcggca aagctggccg acgtgtctat catcatatcc ggcacagact
      121 gggccatgag cgacaagatg agctgcggcc ccgggcgctg ggctgtgacc agatttttcc
      181 aggacgccag gcggccctta tagtcgtcgg actccttgag gaccttcttc aggccttcgc
      241 ggacgcggtt cggttcatcc cagatgatga cgccctgtcc ggcatagtcc aggagcgtat
      301 atttctcgtc atcctggaca gtcagggaaa tgggcaggat gcgggccttt tcccgctggt
      361 cctgggattt ctggctgtct gtatcgaacg tgcgcaggct gtccagttcg tcgccgaaga
      421 actccaggcg caggggctgc ggttcgttga cggcatagat atcgacgatg tcgccgcgga
      481 cggaaaaatg accgcgccgt tcgaccatgt cgacccgttc atagccggcg tcgacgaggt
      541 gctgcaacaa ctcgtcgcgg tcgtagtcct gatggatggc cacgtcgata gcggcggcat
      601 cgatgcgctg cggcgccatg acgtactggg ccgcttcttc cggtgccgcc aggatcagga
      661 gccggtcacc ctggcgcagg cggcccaagg cttccatctg ccgggccgtc cggtccaggc
      721 tcttggccgt cgtcgtaaag acggccttgt cgacgacagg gaaatccagg actggcaggt
      781 ccggtaccaa aaaggccagg tccgccatcc aggccgcagc ctgttcccgc gacgggacga
      841 cgaggacggc ctgccccggc ttttccgtca gggccttggc gacgagggcg ctcttgacgg
      901 aaccgctgag gccgtagata ctatgacagc cgggctggga aaacgcagcc acggcggagc
      961 ggatattttc atcttgattc atccattgaa agaattgctt catatcgttt ttacctcatg
     1021 acaaattggg gctgtcgcat gaagccctgg aaaacaaggc ggtccatgcg cagcccttat
     1081 cgtgattcaa ctgcaactat ttaattatat catagttcac tggcccctgc caggagcgat
     1141 tagaagaccg acgcgccctg catgatgatg gcttcccgtt ctttccggct catcttgagg
     1201 aaattcgtct tcttcagctt atcttcataa gacgaatcca taccgttcat cttctgggcg
     1261 acatccttgc ggatctgcgg tagcaggctg tagaggttgc ggcccgggca gtccgtactg
     1321 ttgacgtcgc gatggcctac gatggtagac ggacccggca cgatgctgta gatcttgcag
     1381 atccaggcga ccagtccttc cagggagtgg atctgggccg gcgtggggat ttccttttca
     1441 aaattgcccg tcacattgat gccgacggta tggtagttct gcccttcggc atgagcaccg
     1501 accgtagcca gcgggcggcc ccgttcaatg gtcccgtctt tgcggatgac gtaatggtaa
     1561 ccgataccgg cccagccgtt ggccagatgg gcgcgatgga tagccgctgc cgacgtatcg
     1621 ccatcgggga tgccgacgtg atggatgacg accatgtccg tcttcggccg caggagcagc
     1681 gtttcgcgga actggaaatg cgtttccttg atggccatcg gttcggccgg cgccgcatcg
     1741 gtcacgggat tatagttgcg catgacgccg cgggctccgg cgggtttcgc cgaccgcggg
     1801 acattgccga tgactgcggg cttggcggct gtgctggctg cagcggctgg ctgggacgat
     1861 acaggcgccg tcttgccggc ctggtctttt ttcgtcttcc ctgccttctt ggcagcctta
     1921 gcggctttgg ccttggccct ggctacggca tccatgtgct ggtcgacact ggcgacgccc
     1981 gtcgtatagg ctgcgccttc attgtcggca gcactcgcca aagaacctgt cgtcatgccc
     2041 agggccagac aaagggtcaa ggccagggca gatatcattt ttttcgtttt cacgatacat
     2101 acacttcccc atgttgttgt ttgattttct ggcagagccg gtcccacagg gcctgcggtt
     2161 ccggatggcc gtacgtcccg agccggatgt gcggcttggc ccggccgtgg taatcgctgc
     2221 ccaaggtcgc catcaggcca tagttatcgg caatgcggcg gtaaaaagca ctcgtccctt
     2281 cgtcgtgata gctgcaatag gcttcgatgc catcgaggcc cgtagcgatg agctcttccg
     2341 tcagcggccg gttctgcttc atattggcac cgggatgggc caggacggcg acaccgccgt
     2401 ggtcgtggat gatggcgacg gctttggcaa aatccatgaa ggtcatgggc acgaaagccg
     2461 ggcctccctg gccgcagata tcccagccaa agttgacgta tggcgcatcg ctccgcgccc
     2521 cacccggccg gtacggtgcc aggacgggac aatccgcatt gcgcgggtcc gccagggcga
     2581 cgcgggcgat atggacggac gcaacggcat cgccgctgca gagcttccag atagccggcc
     2641 ggtcgaggac gatgcccagg gcttcgacgg cgtcgaggac cttttctgaa atcgcccggc
     2701 gctggccgta tacatccttt tcaatggccg caaaaacagg gtcctcggca tggatgccat
     2761 agccgagaag gtggaaattc ttccctttat actggcagct gatttcgatg cccggcacca
     2821 ggttcaagcc cagccgggcc gcttcctgcc gcgcttccgg gatagcccgg gccgaatcgt
     2881 ggtcggtcac ggcgacggtc gtcacgccgg catcggcgca ttggcgcatc aagtcggccg
     2941 gctcgaattg gccatccata ctatatttcg tatgcatatg cagatcaagc catgtttcca
     3001 tacatgcact tcccttcttc aaacatcttc cctattatac tacgcttctc cggcccctgc
     3061 cagtcatgac aaatcaatta ttcgattttt gaattttatc gttttgaaat ctctatcact
     3121 aattttttaa gtttttattt acattttcta aaatcttttc tataatatct ccaacggatg
     3181 caaaaaagtc attacgacgc atctatcgac tacctcatta caataaaata ttcattcaaa
     3241 ggagaatcca ttatgacaga aatgcagcag tatgtagaag acgtgctggc catcatccag
     3301 cgccgtgacc ctgaacagac ggaattccag aacgcagccc gcgaagtact gacgtccctc
     3361 gtgcccatgc tggaaaagga accgaaatat aaacagcacc acatcctgga acgcatcatc
     3421 gaaccggaac gcgtcatcat tttcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnaagagcg tcaacctgag catcttgaag ttcctggctt ttgaacagat cttcaagaac
     3541 gccctgaccg gccttcccat cggcggcggc aaaggcggct cggacttcga cccgaaaggc
     3601 aaatcggacc gggaagtcat gcatttctgc cagagcttca tgaccgaact gtgccgccac
     3661 atcggcccca acatcgacgt accggctggc gacatcggcg tcggcgcccg tgaaatcggc
     3721 ttcctctatg gccagtacaa acgcatccag gacgctttcg atgccggcgt actcacaggc
     3781 aaaggcattg attacggcgg aagcttcgcc cgtacagaag ctaccggcta tggtctcctc
     3841 tatttcgtca aggatatgct ggacaacaag aacatcgaca tcaaaggcaa gacggtcatc
     3901 gtttccggtt cgggcaacgt cgccatctat gccatcgaaa aagcccaggc cctgggtgcc
     3961 aaggtcgtca cctgctccga ctccagcggc tatatctatg acgccaatgg catcgacgtc
     4021 gcaaccgtca aggacatcaa agaagtccgc cgcggccgca tcaaagaata catcgaaacc
     4081 catccggatg cagaatacca cgaaaacagc cgcgatatgt ggaaaatccc ggccttcatc
     4141 gccctcccct gcgctacaca gggcgaaatc gacctggaat cggctaaaat cctcgtccag
     4201 aacggcgtaa aagccatcgg cgaaggcgcc aacatgccgt cgaccctcga cgctatggct
     4261 tacttccagg aacacggcgt cctctttgcg ccggccaaag ctgcaaatgc cggcggcgtc
     4321 gccgtatcgg ccctggaaat ggctcagaac tcggaacgcc tgtcctggcc ctttgaaaaa
     4381 gtcgacgcta ccctcaaaga cattatgcgc aacatctttg aagaatcccg tgacaacgcc
     4441 gcaaaatacg gcgtccccga caactacgtc gccggtgcca acatcacggc cttcatgaaa
     4501 gtcgccgacg tcatgattga acaaggttta gtgtaagata tagaaaaggg attgtcgcac
     4561 atgcgacaat cccttttttg ctgtagggga cgcccagaga gcgtcccgct gtgaattttg
     4621 tgtattccct accatgcagc agttagcgga tggttaacaa tttaaaacca gattatacaa
     4681 actataaaat gaaaaaggac cccgcatgat ggcaaaagcc ttcgtgcggg gtccttttca
     4741 ctattttttt ggtgcaccat acaaaagtta atgcgaactt gacgttacaa ttctatccgg
     4801 attatttcgc atcgtgtatt tccgcatgaa aaagacggcc ctgtcatcgg ccgtcttttt
     4861 tattcataaa aaagggcgtc gtgttttgcg acgccctttt aataccttaa tatgacatag
     4921 ttactgctgg ctatcacctg tcggtgcttc cggctgtgta gctgtcgccg gcgtactttt
     4981 agccagtacg agcttgactg cccaaaggat accgttaatc atcaaaggca ggacgaactt
     5041 atcacggacc atgcaccagc cctgctcgct ctcagcctgg acctgtacct gcgtgataaa
     5101 tttatccgca acggtagaaa gggcgtccat gccgtcgcct gtgaggtcgg taatcaactg
     5161 gtcttttgcg gattcgacga cgtcgctgac gtctaacgcc gataccatac catctctaat
     5221 ttctgtccat ttactcatag tttcgttacc tcctattgta cgctacattc gtaatctgtg
     5281 acaccgcggg caatggctgc ggcgaatgcg tccgtgcagt tttccagcag ggccgcgtcg
     5341 ctgtcgttgt cgatgaacgc ggtttccacg agaacggcgg gcattgatgt gtgcttgagg
     5401 acaatcaggc ccggcatctc tttgacgcct ctgtcgatag tgcctaaggc atcgacaatc
     5461 tggtcttgga tgcactgggc gaggttgccg ccgtcagaca tgcgactgta gcactccacc
     5521 tctgtacctc tggcatggcc attagcggcg ttgcagtgga tgctgacaaa aacatcagca
     5581 tgccattcgt tcgcggtttc agttaccgtc aggccctgtc ggtcttcata gccgctgcgg
     5641 ccggcggacg tcggggcgag attgtcgctc tgcatcatgt agcactcaca gcctgccgcc
     5701 tctaaatact tcttgacggc tgagctgatt tccaaggcta cgtcgcactc gcgcaggtct
     5761 acgtttcctg ctgcatcttt atgtaccgcg ccagagtcgt atttcgtatc gtgtcccgga
     5821 ttgataaata ctttcacttc tcattatctc ctctctttgt gacagccgtt ttagcatttc
     5881 cgccgatgta gccaactagc ccgctgccaa tgctcattgt gagctcgttc atgccatgca
     5941 aaacggaaat gatcagtgtg gccaccaggc cgacgacggc cacaacttca atgatgttga
     6001 ttttttcaat taaattcatt ctccctcctt ctagtttttc agtagcaagt tgatgaagaa
     6061 cgaaataatt ccggcaatgg caccgccaat taaatacatg ccattgattc ggtggtgggc
     6121 gctcctcgct gactggtttg ccgtaatcac catgtctctg acttccttca tttctacttc
     6181 caagctgctt accttcctga tttccgtttc aatacttgca agcctctcca gtacttcgct
     6241 ttcaaaatca tgttctccca tctggtcctc ccttaatatc gtcctgttag ggccatttgc
     6301 agcctttcgg cgctggcaag ctcacgtttt aacgcttcgt caattaaggt ttgctctgcc
     6361 agtgccttga taatcttatt ttgcagccca atga
//
LOCUS       DAWABZ010000105         6355 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_3258_length_6355_cov_3.549048, whole genome shotgun sequence.
ACCESSION   DAWABZ010000105 DAWABZ010000000
VERSION     DAWABZ010000105.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6355)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3258_length_6355_cov_3.549048"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1686..1695
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1573 a         1735 c         1697 g         1340 t
ORIGIN      
        1 ctggtcgtca caggaaagca ggtctttacc ctgccggtca cgggagagac tgtggtccgg
       61 cgctatcaga agtgcctgca cccggcaagg tatgtattca aaaatgtttg attttacggt
      121 ataataaagt gttagattgc ctaaatgaat cattaaaatt gcaagaaaca ggagttgaga
      181 atctctatgt cgaaagacaa agctagatat ggtgccgaac agattcaagt attggaagga
      241 ctggaagccg tccgcaaacg gcctgggatg tacatcggca gtacatcgac acggggcctt
      301 catcatctgg tctacgaagt cgtcgataac agtatcgacg aagctctggc cggctattgt
      361 acgcacatcg aagtaaccat ccaggaagac aacagcatca ctgtcgtcga taatggccgc
      421 ggtatccctg tagccatgca taagatcggc aagcctgctg tcgaagtcgt cctgacggtc
      481 ctccatgcag gcgggaaatt cggcggcgac ggctatccca tttccggcgg cctccacggc
      541 gtcggcattt ccgtcgtcaa cgccctcagc gtctggacga aggtccaggt caaacgggat
      601 ggttatttat gggacatctc ctttacccgc ggcgtgaccg atcagccgct ccggaaggta
      661 cgccctctgg aagcaggaga agaaacgggg acaaaagttt ctttcaaacc ggaccatgaa
      721 atcttcccgg acacggtcta tgacttcaat accttgaaga cgcgcctgcg cgaactggct
      781 ttcctcaata aaggcctgca catcaccctg accgataagc gcggtgaagg caagagcgaa
      841 gtctactgtt atgaaggcgg cttgacgtcg ttcgtcgaat tcctcaacga aagccatcag
      901 gccgtcaatc cgacggtcat caacgtcgac ggcgaaaaga acaaggtcat catcgacatt
      961 gccatgcagt ataacgacag ctacagtgaa aatatcctga ctttcgtcaa cgatatcttc
     1021 actgaagaag ggggcacgca tctgagcggc ttccgttccg gcctgacgcg tacgatcaac
     1081 gattatggcc gcagtgccgg catcatcaaa gagaacgaag acaacttgtc cggcgaagac
     1141 gtccgcgaag gcttgacggc ggtcatcagc gtcaaagtac gggaaccgca gttcgaaggc
     1201 cagaccaaga ccaaactggg aaacagcgaa gtcaagggca tcgtcgacaa tatcgtttcc
     1261 gacgggctga aagaattctt tgaagaacat ccatctgaag ccaaggccat catcaataag
     1321 accatttctg cgtcccgtgc ccgccaggcc gcccgccgcg cccgcgagct gacgcgccgc
     1381 aagaacgccc tggaaatcag cagcctgcct gggaaactgg cggactgctc ggaaaagaat
     1441 ccggaccaga cggaaatcta cctggtcgaa ggggattcgg caggcggcag cgccaagcag
     1501 ggccgtgacc gccgtttcca ggctatcctg cccctgcgcg gcaaaatcat caacgtcgaa
     1561 aaggcccgcc tcgacaagat cctggccaat gatgaaatcc gttccatgat cactgccttc
     1621 ggcacgggca tcggccagga attcgacttg agcaaacgcc gctatgacaa gatcatcatc
     1681 atgacnnnnn nnnnncggcg cccacatccg gacgctgctc ctgaccttct tctaccgctt
     1741 catgaagccc ctcatcacag acggccacgt ctatatcgcc cagccgccgc tctatcaggt
     1801 gcgcaagggc aaacaggtct ggtacttcta tacggacgaa tcgatgcagg ccaagctcga
     1861 tgaactgggc cgtgacagca acaacgtcgt cgtccagcgc tataaaggcc tgggcgaaat
     1921 gaacccggaa cagctttggg aaacgacgat ggacccggaa aaccggacca tgctcaaagt
     1981 cgaattggaa gacgccgccg aagcggaccg cattttctcg gtcctcatgg gcgacaaagt
     2041 cgaaccgcgg cgcaaattca ttgaagaaaa tgccaagaaa gtccgcaatc tggacttgta
     2101 agaggagggc ctatgccgga catttcccga aagatagact tattcactga ttccgtcatc
     2161 gcccgcatgt cgaaagtggc cgaagcctgc ggcgccatca atctggccga aggcttcccg
     2221 gactttccgc cgccccaggc catgcgcgac cgcctcagcg aagtagctca ggccggaccg
     2281 caccaataca gcatggataa tggcgcagcc aatctgcgcc aggccatggc cgactgccgc
     2341 cgcatgttca cgggccagac catcgaccct gaacgggaag tcgtcgtcac ctgcggcggg
     2401 acggaagccc tcatggcggc catgatgacc ctcgtcgacc ctggcgataa agtcatcctc
     2461 ttttcgcctt tttatgaagc ctacagtaca gacgccctgc tctgcggggc agagcctgtc
     2521 tacgtatcgc tccgcgaacc ggactatacc tttgatgccg atgaattgga agcggctttc
     2581 aagcagcatc cgaaagcgat tttcctctgc aatccgtcca atccgtgcgg caaggtattc
     2641 tcccgggaag agctgatgac cattgccaag ctggtcgaaa agtacgatgc cttcgtcgtc
     2701 atcgatgagg tctacgagca catcatctat gaaccgacac agtatacgta tttttctacc
     2761 ctgcccggca tgtatgaacg gacgctctgc tgcggttccc tgtccaagac gtattccatc
     2821 acgggctggc gcctgggcta tattttcgcc tcgccggctc tgacggatgc catcaagaaa
     2881 ctccacgtct acctgaccat cagtgcgccc agcccgctgc aggaagcggc cgtgacgggc
     2941 ctgcgcttcg gccgggacta ttacgatggc ctgcgggcca tgtatacgcg caagcgggat
     3001 ttcgtcatga agcgcctcga tgacatcggc atcggccata atgtgccgca aggggccttc
     3061 tatatgctcg tcgatatcgg cggttatggt tatgatgacg acgaagcgtt ctgtcgggac
     3121 ctggcagaaa aagttggcgt cgccttagtg ccggcttcga cgttcttcat cgaaccggtc
     3181 catcacctgg cgcggctgca attcgccgtc cgcgatgaaa ccctggaaaa ggcgatggaa
     3241 cggctcgaaa atatttctct attgaaaaaa taagttgcct atggcaacca gggggaggga
     3301 tttttgatga aaaagtggca gattctctta ggagcacttt gtatcagcgt atcgagtctg
     3361 acagcctttg ctgccgatga gcccgccaag gcggagccgg cagcggctat cgtcctgacc
     3421 gacccgacgg tcaacgtcaa gaacctggac gatttttata tcctcaaagg gagtacctat
     3481 gataaagtga cgggactttt gcaaaatctg ccgtcccaga cgacaggcct cgttggcagc
     3541 tattatttcc gcgtcgacaa gaatgtcgtc ggcgattact tttatcacgt caaggcctat
     3601 gatacggtat cccacgtcct cataggcaat tatttcgtcg ccaaggatga aagctgcgcc
     3661 tggaaactgc aggccggcga cgatgcggcc ctgatttatg gcaatgcaga aaaactgatg
     3721 gataaggtcg aagtcgtcgt ctatccgaag aagatccccc tgggcagcta cggcatcatc
     3781 cgcgtccatg taccggggat ggtaccctat gacgtcaaag cgacgagttt gaatacgaaa
     3841 gtcgccgata ttacggaaaa aatgaatatt catcccatgc aagccggcaa gaccgacttg
     3901 gtcatcgaca tcaagatcgg tgacactgtg cgcactgtga ccaagtcgat taccattgtc
     3961 gatgaagccg accgccatac gggtgaaccg tcccatagcg gccctagtgt cggcgtaggc
     4021 atcggcatcg gctggggcgg cggctggcac caccacggcg gtggtatcgg tattggcgtc
     4081 ggcccttggt ggtaaccatt taaaaggagt gtgtatctat tgtggagttt tccctaggaa
     4141 aacaatttct cgtcatcatt ttgctgattt ttggcaacgg catcttttcc ctcttggaaa
     4201 tggccatcgt cagctgtcat caatcccggc tggaagccct ggccgatgaa ggcagcaagt
     4261 cagccgctat tgtcctgaaa cttcgtgaaa atccgaataa gatgttttcc atggtccagt
     4321 tcggcattac cctggtcagc ctgctcaccg gtgtttacgg cggaaccacc atggccggcc
     4381 ctttgtcgaa gtatgtagcc caaattcctg gcttggaagc ctatgcctat acgatcagcc
     4441 tgacgggcat cgtcgccttc attacctact tgacgattat cctcggtgaa atcgtgccca
     4501 agcgcatcgc catcgacaaa ccggaaaaaa tctcctgtct cctggcccgg ccgatgctct
     4561 atttttccat tctctgtaca ccggtcgtat ggattttgtc ctggtcgacg gcgatggtta
     4621 cgaaaatcat gggcgtcaat aaaccggaag tcctgcccgt tacagaagat gaaatcaagc
     4681 tgctcctgga acagggcgtc gaactcgggg cctttgaaaa agaggaaccg gaactggtag
     4741 atcgcgtctt ccgcctggcc gacatggacg tcggtgatat catgaccaac cggacccagg
     4801 tcgattggat tgatgtcgaa gatccggaag aaacaatcat gcaggaactg gtagccttta
     4861 accacaacac tctgcccgtc ggccggggct cgctggatga tttcatcggc atgatttccg
     4921 tcagcgaaat cttccagaaa tactatgccg ccgtccggaa ggataagacc gtttccctga
     4981 cgaccatcgt cgaagagagc gtccacaagc cgatttatat tcccgaatcg atggacatta
     5041 tgaaagtcgt ccacgtcttt cgcgaacaca gcgtccatga agtcgccgtc ctcgacgaat
     5101 acggcaattt cagcggtatt ctgaccctgc atgatatcct ggaagaactg gtcggcatca
     5161 tgccggctgg cgaagaagag aaaaaggagg aagcgaaccg catcattcag cgcggcccta
     5221 atgaatggct catggaaggc ctgctgacca tcgaagagtt caaggatttc ttcgacatcg
     5281 aagacgattt gcccgacgaa gacgaagatt tgtataagac cctgggcggc ctcatcacct
     5341 atggtgtcgg ccgtatcccg cgggaaaccg atacgtacga gtgggaaaat ttcacttttg
     5401 aagtcatgga tatggacaac ctgcgggtcg ataagatact ggtgacccgc agagatacac
     5461 cggatgccga tgaaaaagcg gataaaggtg ccgataaatg attaggtctt tgagaaagga
     5521 agtttgtatg gaaatgggag aacgccaggc aatttaccag gtgtctacgc tgcaggccct
     5581 ggcccgcggc aattattacg gcagtaccga tatggaaacg ctgctgaccc atggcgacac
     5641 ggggctgggg acgtttcacg ccgtaggcgg tgaaatcatc gtcatcgacg gccatgctta
     5701 ccgcgtctta ggcgatggat cggctgtgga agcggccacg agcgatacga cgccctttgc
     5761 cacggtgacg tggctgcggg aacaggttcc cgtcatattg aagcgccagc cgtcgattga
     5821 agccctgaag gatatcctga ccgagcaggt cagggaactg ggcatcaatg atgtctacat
     5881 cgtccgcatc gacggccatt tccgcagcgt atcggcccgg accgagctgg aacagcagga
     5941 gccctatctg ccctttgcca aggtcctgga aaaagatgaa cgccgcttca cttttgaaga
     6001 tatcgacggc agcctcatcg gcatctacat cccggactat ctgagcggcg tcaatacgtc
     6061 gggctggcat tcccacttca tttcggcaga ccggaaaaaa ggcggacacg tctttgacct
     6121 ggacctggaa gaaggccgtg tcattttcaa caaagccgac cgcttcattc tcgacatgcc
     6181 ccataataaa cagttcgaac ggggcgacct gacggctgcc tcacaggaag aaatcaaaca
     6241 gattgaacaa gggaaataaa aaaataacct gtcattagca ttttttgaca ggttattttt
     6301 tatttcgtat aatatcatca ggtgttgtca cgaacaatag gcggttactt aagcc
//
LOCUS       DAWABZ010000106         6316 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_3293_length_6316_cov_2.796838, whole genome shotgun sequence.
ACCESSION   DAWABZ010000106 DAWABZ010000000
VERSION     DAWABZ010000106.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6316)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6316
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3293_length_6316_cov_2.796838"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6034..6043
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1426 a         1747 c         1776 g         1357 t
ORIGIN      
        1 ccgttataga ccaggggctt ggtgctgtag accgtgccgt tgaccgcatc tgccttgggg
       61 gccgaataga tttcttccgt cgggatgttg gcattgaaca gcaggtggtc cgccgtttct
      121 tccatgccgc cctgccagat atgaccggcc ggcaggccga tagtcaggtc cgttcccagg
      181 ccgttcgtat agtgcaggct ggtgaaatgc tggtccgtca tccaggtccg gcgcttcttc
      241 aattcggcca gatgggcgtc ccaatcggcc agggcatcgg caccgtcggc acgggacgac
      301 tggaggattt gcttccacaa ggcttcgtaa gcggcggctt cgtcgaggtc cggatagagc
      361 agtttggccc agactgcact ggggacggcg gcgacgagcc acgtcgtctt cgaggccatc
      421 atgccgtcga tgtattcctt caaggcttca ttctgggctt tctgctgggc aaagatttta
      481 tgggtgtcga tgccgtccat gaggtacgga ttcgccgaga tgaggctgag gaaggccgct
      541 ttcttatggt aatagtagag gctgaaatcg cggcgccagt ccggcaggga ctcgaaatac
      601 tgcaaatctt cgtgttcgta gcgcaggcgg gccaggtcgt cagaccgcca gttgcagacg
      661 acctgggcgg cgccggccgt ataggcctct ttcgtcagga gggcgacgaa gtcgtgcgat
      721 tcgacagggg cattgatgac cagggtctga cctttctgga gattcaggcc cttctgcaag
      781 aggacgcggg cataagccgt taattgtttg gtagttggca taaaatcact ccttagtaga
      841 tgaggatagg acagggactt tccaggccgt atgcttccac aagcgccagt tcagggcgct
      901 ggcattgatg gccaggctga tgatgaggct gatccaatag ccatagggcc cgtaggacgt
      961 gacgtgggcc atggcatagc ccatagggat gctgatgagc cagtaggtca cgaaagcgac
     1021 gtaagtgatg atcctgacgt ccttatagcc gcgcaggatg ccctggaccg gcgtgccggc
     1081 ggcatcgaag aaggaaaagg cgacggcgta gaagatgaac atgcggatgg cccagaacgt
     1141 ttcggcatcg ctcgtgaaga tggcggcgat atgatcgaca aagtggtaag tcacgacgat
     1201 gagggcgatg gcgatggtga aggccgtcat ctggcagagc acggcatact gccagatgac
     1261 ggcgtagttg cgggcgccga cttcgtagcc gatgacgata gtcgccgtca ggctgatgct
     1321 ccagggcagg gtataggtca gggtcgagaa actgatggct gcctggttgg ccgccaggta
     1381 taaggtgccg aattcggcca tgagcaggcc gacgatggag aacaggctcg tttcgcagaa
     1441 gacggcgatg aagatgggaa tgcccagctc cagctgatga tagccatgat accagctgaa
     1501 aggccgcagg cggctccaga gatggtaaga gcggaagggc tccatccact gcaggacgcc
     1561 cacgaagagc aggaaggaca gccacgacgc gatggtgatg gcatagcccg taccgatacc
     1621 gcccatagcc ggaatggggc cggcaccgaa gatgagcagg cggaagagga tgatcgtcag
     1681 gaccaggttg gtcactaaga tggccatgga aacgtgggtc ttgccgtggg cgtcgacgat
     1741 gtagcgcatc gtcgcctgca ggcagatggg gaccagcccc agggccagag ccttgaggta
     1801 ttcccaggcg atgtagcgga ccggtgcgtc gaggcccata aagtcgagaa agggcggcag
     1861 cagaaaggcg ccggccagga aaatgacggc cgtaaagccc aggctcaagt agaggctctg
     1921 cataatgtac gtcgggattt cctcggtctt ctgagcgccc cggagctggg cgatgatcgg
     1981 cgagatgccg aagaagatgc cgcacaggcc ggcaaagacg ggataccaga tattgacggc
     2041 tacggcgacc ccggccaggt caatcgtccc ggcctgaccg ctgaagatag aactgaagac
     2101 ggatgtcccg acctgggcca tctgggtcag gagcagggga aagaacaggg ccaggaaatg
     2161 gcgtaattgg aaccaatggg ggaacatgat atcctccttg tatgaaaaag ggcttgtcgc
     2221 acaacgacaa gcccttttac gtagctagtg gctagccgtt agtggttagc ggatggcttt
     2281 aaattttaac gctaactgct agctgctgac ggcttaaaag agctagtgtt tcgacgatga
     2341 acttgcgttt actttttgtc aagtatatat gcgaaatagg tacggttaga cgtaactctc
     2401 atccttctca tttgcctagg gtatgttaaa ataggaacgt actgtctcat acttatttat
     2461 tcctatagta aaaggaggaa ttttatgaaa gaccaagtct tttccatctt gcaggctgtc
     2521 ggccgcagtt tcatgctgcc tgtcgccatc ctgccgattg ccggcttact cctcggtatc
     2581 ggggcttctt tcacgaatgc tacgaccatc cagacctatg gattggaaag catcctcgga
     2641 aacggcacga tactccattc actgctcacc atcatgagcg atgccggcaa catcattttc
     2701 ggtaacctgc ccatcatctt cgccgtcggc gtcgccatcg gcatggccaa gcaggaaaag
     2761 gaagtcgccg ccttatcggc gatgatcgcc tttttcgtca tgcacgcctc gaccaacgcc
     2821 gtcctcaaac tgactggcaa ggtattggcc aataacaccg tatctcccga tgtcctggaa
     2881 ggtacgattg ccaatgtctg cggcatcatg tccctccagg aaggcgtctt cggcggtatc
     2941 atcgtcggca tcggcgtcgc ctacctgcac aaccgctatc ataaaatcgt actgcccaat
     3001 gccctgtcct tcttcggcgg gtcgcgcttc atccctatca tttccaccgt cgtctacatg
     3061 ttcgtcggca ttgccatgta tttcgtctgg cctgtcgtcc agagcggcat cttcgccctc
     3121 ggcggcctgg tcacgggtac gggctacatc ggcaccttga ttttcggcat catcaagcgg
     3181 gccctcatcc ccttcggcct gcatcacgtc ttctacctgc ccttctggca gaccggcgtc
     3241 ggcggttcga tgatgatcga cggcaacctc gtccagggcg gccagaacat cttcttcgcc
     3301 cagctcgctt cgcctaacgt cactcacttc agtgctgatg caacccgata tttctcgggc
     3361 gaattcatct tcatgatctt cggtctgccc ggcgccgccc tggccatgta ccgcacggca
     3421 aaaccggaaa aacgcaaagt cgccggcggc ctgctcttat cggcagccct gacgtccatg
     3481 ttcacgggca tcacggaacc gattgaattc tccttcctct tcgtcgcacc gatgctcttc
     3541 tacgtccagg tcgtcctggc cggtgccgct tacatgatcg cccacatgct gaacatcgcc
     3601 gtcggcctga ccttctctgg cggcctgttg gacctgttca tcttcggtat cctgcagggt
     3661 gaagccaaga ccaactggat gtacatcatc ccggtcggca tcatctactt cttcctctac
     3721 tacttcatct tcacgttcct catcaagaag ttcgacctca agacaccggg ccgtgaagac
     3781 gacgatgtgg aaacgaaact ctacaccaaa gccgacgtca aagcccgtga cgccgcagcc
     3841 aaagccggcg acggcgaggc tgccacggac gtcgtcagcc aggccatcac ccgcgccctg
     3901 ggcggcacca agaacatcac ttccgtcgac tgctgcgcta cgcgcctgcg ctgctccgta
     3961 gccgattcgg ccttggtcaa cgatgcagct ctgaaagcca ctggcgctgt aggcgtcatc
     4021 aagaaaggcc agggcgtcca ggtcgtctac ggcccgcaag tcgccgtcat caaggccaac
     4081 ctggaaagct atctggcaac caacccggca gacggcccta tcgaagccgt accggcagcg
     4141 gaaacggttc cggctgaaga agcgccgaaa gcccaggaaa cacagactga accggaaaca
     4201 gatgctgaaa agcattactt caattcgccc ttcaccggca gcctccatcc cattacggaa
     4261 gccccggatg aagcctttgc cggcaaaatg acaggcgacg gcttcttcgt ctatccgaca
     4321 gacggcacgg tctatgcgcc ggaagacgga acagtcacct tcgtcttcga tacgaaacac
     4381 gccatcggta tgaccacggc agacggcgta gaatacctcc tgcacatcgg catcgatacg
     4441 gtcaaactcg aaggcaaagg cttcaatgtc ttcgtcgaaa acggccagga agtcaagaaa
     4501 ggcgataaac tgatggaatt cgacgacgaa ttcatcaagg ccaccgcccc atctgacgcc
     4561 tgcctgtgca tcttcaccga cctcgaagac ggtcaggaag tccacctggc agccgacggc
     4621 aacgtaaaag ccggcgacca ggccgtctgg ttctaatgtg taaaaccgca ggccgcaggc
     4681 aagcctctcc tgataggaga ggtggcccgc agggccggag aggttgaacc cttcagtcag
     4741 cggcgctgcc tgctctcctc ataagagagc caacgggcgt gagccacgag cccttacaaa
     4801 aaaggcgctg tcgcgatgcg acagcgcttt ttttagcagt tagcggctaa tggctctccc
     4861 gggattccca catggcccga gacgggccgc ccacgtgcgg cccctgcgac taaccactag
     4921 ccactaatac agtttcttgt aaatgccgta caccttatca taatcgaggg gcacaaagct
     4981 gtgcttcatg agacgctgct gattttcgat gaccgattta gcaaattccg gcaattcttc
     5041 ttctttgacg ccgtattcat ggagcgcttt cttgggcagg atgacgttga gcagtttttc
     5101 cagttcttca tagacgtgtt ccggttcgca gcccaggatg tcggcgatga agccgttcaa
     5161 tttcgcgatt tcaccgtccg atttgatgga catgtagcag ttcatgacgc ctgtgaacat
     5221 ggcgtagttc gattcaccat gaggtacatg atactttgcc cccagaggat agctcatggc
     5281 atggacagcg gcgcagccag ggacactgaa agcgatgccg gcccagttgc tggccaggag
     5341 gaaagccttc ataagcggga tgcgcgcttc cggaccatgg tcgcggatct gcaaatagcc
     5401 cttgaggatg agttcaatgg cgttataact gaaaacgcgg aagctggcgc tggaatccgg
     5461 agacaaggcc gattcgacag cgtggacgag ggcatcgatg gaactggtcg caaagacctt
     5521 gaaaggcagg gtcttcaaga gctgcgaaat gaggacggct ttatcggcat agagcgcatt
     5581 gtcggcaatg cccttcttcg tgttgcggct aaccagggcc atgaccgata cattggtgac
     5641 ttcactgccc gtcccgcacg tcgtcgggat gaggatgagt tccttttcct tttccacagg
     5701 aatcttgcca tcgaacaaat cgaggaccgg gctgacctgc ttcaagatga acaacttgca
     5761 caagtccatg accgtgccgc cgccgatacc gatgatgcgc ttcacatcct tgggcatatc
     5821 tttataaatg gcttctgcca tttcatcaga cggttcaccc ataccatact tttcctggaa
     5881 caagacgtgg cacgtaaggc ctaaatcctt gaaattcggg tcgaaaatat actgattggt
     5941 aacaatcagg tcatcggctc cgacctgcag gtcttccaca aattcccggc acgtatcgta
     6001 agagtaaatc gtcggtttca actgtaattc aatnnnnnnn nnncaatcat ttcattaact
     6061 cctttctaat gggtaatgca ctcaccgcct ttagtgtaac aaagatggaa acagttttgc
     6121 aagatacagg atgcccgtga tataataaac ctgtcctggg ggaggatggg ttctggtgtt
     6181 ccctctggtc ttcaaaacca gcatggggag ttaacagctt ctcaggtggg ttcgattccc
     6241 acactttccc gccagaaaac gaaaaagggg ctgtaacaaa atgagttttt ttgctcattt
     6301 tgttgcagcc ttcctt
//
LOCUS       DAWABZ010000107         6256 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_3331_length_6256_cov_2.981938, whole genome shotgun sequence.
ACCESSION   DAWABZ010000107 DAWABZ010000000
VERSION     DAWABZ010000107.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6256)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3331_length_6256_cov_2.981938"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1404 a         1547 c         1807 g         1498 t
ORIGIN      
        1 attgagaatc cggcaatttt cttggaaacg aatatcatgg ggacggctgt cctcatggat
       61 gcctgccgca agtacggtat ccagcggtat catcaggtat cgacggatga agtttatggc
      121 gatttgccct tggaccggcc ggatttgttc tttacggaaa cgacgccgat ccacacgtcc
      181 agtccgtata gttcctccaa ggcttcggct gacctcctgg ttctggctta tcatcggact
      241 tatggcctgc cggtgaccat cagccgctgc tctaataact atggtcctta tcattttccg
      301 gaaaagctga ttcccctcat gatcatcaat gccctgcatg acaagccgct gcctgtctat
      361 ggggacggcc tcaatgtccg cgactggctc tatgtcgaag accattgccg ggccatcgac
      421 ctcatcctcc agaagggcca tgtcggcgaa gtctataata tcggcggcca taatgaaatg
      481 agaaatatcg acatcgtcaa attgatttgt aaagaactgg gcaaaccgga aagcctcatc
      541 caccatgtta cggaccgtaa aggccatgac cgccgctacg ccattgaccc gacgaaaatc
      601 cataaagaat tgggctggct gccggaaacg aaatttgccg acggcatcaa gaagaccatc
      661 cagtggtact tggaccacga agattggtgg cagcccatca tttccgggga ataccagcag
      721 tattatcaga agatgtatgg gagtcgaggc taggagcctc gacgcaggcc gcagttcgcc
      781 gtccgcaggc tgtctcggac tcagatgccg ctacgcggcg acagactcaa agtgacaacg
      841 acgcttcggg aaaccctcag cgcgtagcgg gccgccttat taggacggcc cctacgaaat
      901 cgcattcagg cgatgaaaaa attatgcggc tacaccgcat ctgtaggggc cgtcccaaag
      961 ggcggcccgc tgtgaacagt tgggaatatt tccaaactgt tcatatggca tgcagtacgt
     1021 tatgcatcgc tattccggac gggcgccccg cgtggcgccc ctacaaaatc gcatagaggc
     1081 gatgggaaaa gaacctctca gtcagctgtg ctgacagctc cccttcgcag gggcgccttt
     1141 catcgcaggt cgccgcaatc ttgcagacca taagaaatac aaggccccca atcagggcat
     1201 ttttacgagc cgagggttac tcgaggcgat ggtgttactt tgaatttatt gccgcgaagc
     1261 ggcatctgaa tccgacgcgg cctgcgaatg gcgaactgcg gcctgcaaat tgaatatgct
     1321 atgataaaaa ggaacatgca tgaagggaga gaacacatcc tatgaaaata tttgtaacag
     1381 gtgtgaatgg ccagttgggt catgatgtga tgaatgagct ggctttgcgg gggcatgtcg
     1441 gtatcggtag tgatttggct ccggcttata gcggtatggc tgatgggacg gcggtgacga
     1501 cggcgccgta tgtgtctttg gatattaccg atgcgaacgc ggtacggcgg gttattacgg
     1561 atgtacagcc cgatgcggtc attcattgtg cggcttggac ggctgtcgat atggccgaag
     1621 atgacgacaa agtggctcag gtccgggcgg tcaatgccgg tgggacacag catattgccg
     1681 atgtctgcaa ggaattggac tgtaagatgc tctatctcag tacggattat gtgttcaacg
     1741 ggcaggggac ggagccgtgg cagccggact gcaaggatta ccagcccctc aacgtttatg
     1801 gccagaccaa gcttgaaggg gaattggccg tttcgcagac gctggaaaaa tattttatcg
     1861 tccgcattgc ctgggtattc ggtcagaacg gcaagaattt cgtcaagact atgctcaatg
     1921 tcggtaagac ccacgatacg gtccgcgtcg tcaatgacca gatcgggaca ccgacgtata
     1981 cttttgattt ggctcgtctg ttagtcgata tgattgaaag cgataaatac ggctattacc
     2041 atgcgactaa cgaaggcggc tatatttcct ggtatgattt tacctgtgaa atctaccgcc
     2101 aggccggcta tacgacgaaa gtcatccccg tcacgacgga agaatacggc ctgtcgaaag
     2161 ccaaacgacc ctttaattcc cgcttggaca agagcaaact cgtcgccaac ggatttcggc
     2221 cgctgcctga ctggaaagat gccttgaagc ggtatttggg gaatttagtg gctcgtggct
     2281 agtggttcgt ggctcgttga cgaacgacct acgatgaacg ataagggcgg cctgcgaacg
     2341 gcgacctgcg gcctgcgttt acttaaatta tgttataata aaggaaacat gataaaggaa
     2401 acatgcatga agggagataa atattaatgg gtcagataaa ggttgaaaag aatgtgggcg
     2461 gtattgacgg gctttgtgtc attacgccaa cggtgcatgg cgatgcacgg ggttatttta
     2521 tggagacgta taatcagaag gatatggagg aagcggggtt ccatattaat tttgttcagg
     2581 ataatcagtc catgtcgacg aaaggggtct tgcgggggct tcatttccag aaacattttc
     2641 cacagtgcaa gctcgtccgt gctgtccgcg gcagtgtttt cgatgtggct gtcgatttgc
     2701 gccgtgattc caagacgtat ggcaaatggt acggtgtcga attgacggaa gaaaataaga
     2761 agcagttcct cattcccgaa ggctttgccc atgggttcct ggtcctcagc gacgtggcgg
     2821 aattctgcta taaggtcaat gatttctggc atcccaatga tgaaggcggc atggcctgga
     2881 acgacccgga aatcggcatc cagtggcccc agctcgtcgg cgaatatcag ggttcgccca
     2941 gcgccgccgg ctatcacctc gaagacggga cgccgctgaa tctgagcgat aaagaccagc
     3001 agtggctggg gttgaaagat accttttcct tctgattgcc tgaaagcggg gtgagcttca
     3061 tgcagaacat cattgctgtg atttgggatt tcgacaagac tttgattgac gggaacatgc
     3121 aggacccgat ttttgaggaa tatcatattg ataaggcaaa attctggcag catgtcaatg
     3181 ccttgccggc gtattatgcc aaggaaggcg tcaaggtcaa tgccgacacg atttatctga
     3241 atcagttcat caaggacgca cagaatggga ttttcaaggg ccttaataat gaaaagctga
     3301 agacctttgg ccagaagcag aagtattatc acggcgtccc ggaaatcttc aagaatcttc
     3361 agcgcgtcgt cgaagagaac cccaagtacc gcgagtacaa catcaaggtg gagcattaca
     3421 tcgtcagtac gggcatgtcg gctatcatca aagggtcggc cgtcatgccc tatgttgaag
     3481 acatctgggg ctgcgacctc attgaagggc tcgatgcaga aaagcataaa atcatcagtg
     3541 aagtgggcta tactatcgat aatacgacca agacgcgggc cttgtttgaa atcaataagg
     3601 gcgtgcctca gcatccggaa atcgatgtca atgccaagat gtccgatgcc ctgcgccgcg
     3661 tcgctttccc caatatgatt tatattgccg acgggcccag cgatgtgccg gccttttcgg
     3721 tagtcaaaaa attcggcgga tcgacctttg ccatttatcc caaaggaaac agcaaggcct
     3781 tccgccaggt cgaacaactg cgggaagatg gccgcatcga tatgtatgcc gaagccgatt
     3841 accgtgaggg tacgacggcc tacatgtggc tgacccacaa agtcgacgaa atcgccgaac
     3901 gcatctacaa aacagaaaaa gaaagcctgg aaggcgccgt ctcggctgct cccagacatt
     3961 tggtgtgagg tgtttgaggc ttgatgaaag gctctcctaa taggagagct ggccgccaaa
     4021 ggcggtctga gaggtttcgc tttaaagtag gggctgttgc actaaggttt cttccatcat
     4081 gcgaggcccc gttcgctttg tagtttgtag aagctggttt caaattttta accatccact
     4141 aaccactagc cactagctgc taaccactta aaaggagctg tcgttcgaca gctccttttc
     4201 tatctccgcg gtggcggccg atggccgtgg cgtggcggtg gtggcgggcg atgccagccc
     4261 cagtcattat aaaaagggct gccccaaccc caattccaat aggtatccat ataaagctgg
     4321 gtcatgtagg cgttgtaggc catgcgctgc aggtcggcct gggtgacgac ggagatgcgg
     4381 cgcgatgccg tttctttgac gtcgccgacg gtcacgtctg ccaggatgtc tacttggccc
     4441 gtcttgacgg ggatgatggt attgtcttct ccgattttgg cgacggactc attcaggctc
     4501 ttggccgtca ggccattgcc cggcgtctgc agggcgacga tgcctttgct gcccagggcc
     4561 aggtagcgcg ggtagatgat gatgcggctc tttttcatca tttttttagc gctgccataa
     4621 atcaagcctt cgtggggcgt atccatgcgc cagacggcac tcttgtcttt ggcgactaag
     4681 tattcgccaa tctgttccgt cgttttgtcg tcataggccc ggacacggcg gaagtacttg
     4741 tccgtcaggt ctttgtcgac gcggaaataa taaggcctgg ttgttgtgtc cagcccggtc
     4801 cgctgggaat cgatggtctg gagcagcgtc gtcacgtctt tataggtcga gcctttggtc
     4861 aggtaatatt tgtcgatgat tttacctgta tggccggcta acggatccgt aggcacactg
     4921 gctttttctg ctgttttttc agatcctgtt tcggtggcct gggtctgaac ggccgcttcg
     4981 gcaggtgccg ttttggccgt gtctgcagcc agtcctgtca acgtcgtaca cgtgcagaac
     5041 aaggcggcca ggatagcctg tttccatttt ttcacgatac cacctcctca tgcctttact
     5101 atagcgcgcc gctgcggcga gcgtcaatat gccgtaaaga atcttatggg aaatgcaatt
     5161 atttgttcaa ttttacaaaa atgttgcaaa ataaaagtca gtggctctta ggcttactcg
     5221 cccaaggcgt agaggatgtc ggctgcggca gcgttcaggt tgtagcggct ggtgtagcag
     5281 acgagggtgc cctgggaagc ggcctgctgg caggctttgc ggaccagggc gtggcctgta
     5341 tggttggtgt tgatgatgag tacgtcggcg ccctggagtt tttggacgtc gaagtgcttg
     5401 ttttccagga tcatccagtg cgggaagcgc gtgcggactt tgtcatgcca cagggcgctg
     5461 ccgccggcca gacagacgcg gaagccttcc agggcctttt ccaggcgcga cgtgtagtcg
     5521 tcgggcaggg gaagggccct tttttcgacg tcttcctggg gacagcgcgt ttcccattcg
     5581 cgcagggatt gttccagggt ctggacgtag ccatcataac tttcctgcaa ggtctggtaa
     5641 taggcctggg tctgggccag ttcgtcgcgc agccgggcgg catcgtcgtc ggcgacgggc
     5701 ggcgtgtcgt catagaccag gcggatgacg cccaggccca gggcgatttg accggccgtg
     5761 tccaggacgc ctgccaggct tagggcttct gctgtgctcg acggtgtgag gggatggctc
     5821 acggcgatat ggccgcggac tttgtggccg ccgatggctg tcggccgcgg gaactgttcc
     5881 agcgttatcg tggcgatatg acgctgctgt gcggcctccg tgcgatacag ttcgtagacg
     5941 gctttgcgcg gcgtgaagga ctgctgcatc agctgacacg tgccgtccat gtggcccgtc
     6001 tggtctggga attcggccgc gccgtcatcc tgcatctttt tccacagccg ggctgtcggt
     6061 acggcgcggc ctgccaggca gagggcttcg ccataaggca ggaagcgttc ttcgccgcgg
     6121 cacgacaggc ctgccgcttc attggtatac ataatctgtg tgattttcat gataattctc
     6181 ctcgtatcta taacttatgt ctattttgaa cctctcccgc ttatagaagc gggagattct
     6241 tgagaaggtt gctgtt
//
LOCUS       DAWABZ010000108         6142 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_3403_length_6142_cov_3.322983, whole genome shotgun sequence.
ACCESSION   DAWABZ010000108 DAWABZ010000000
VERSION     DAWABZ010000108.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6142)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6142)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6142
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3403_length_6142_cov_3.322983"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1259 a         1882 c         1695 g         1306 t
ORIGIN      
        1 tttgacaatt ctgtgtcgaa tatcttctgt gtagaatatc ttaagataaa ctagttatat
       61 agagttgctt ggaggtagtc aatatgacac tcagtttatt ttggtcgcgt tatacgctgg
      121 aacttcggga acagcatctg cgcgatgtcg atgaacttta tcattatctc attgcccggg
      181 aacgatggaa ctggttcctg gccaagattc cggaacaggc ccagatccag atactccgcg
      241 gccacaacca tggcgaagat tcctggacgt gccgcaagtg gctcgaccac atgctggact
      301 ggatcaagga gaacaagccg cctgccgtct atgatgccgt catcgacaag attcatatga
      361 ttgaagacaa gccgattgaa gaactagaaa aacaggctgc ccgcaacatt tcgccggaag
      421 agttggaaaa actccagcgt gccggttatt ttcaatgtat cgaggcgccg aacaaaagtg
      481 aatgaaagca ggtgagggaa tgacctttct attacgcatg ataacagctg ctgtcttatt
      541 cttttccctg gcagctgccg caccggctgc tgtcgtgcag gccgattccg tccgcgtcct
      601 gcctatttcc ggtgacatcg acggcagcca ggccgccttt atccagcgcg gcctgcgcca
      661 ggccgccgaa gaaggcgata cggccgtcgt cattaccatc aataccctgg gcggccgcgt
      721 cgacagcgcc ttgaagatcc gcgacatgct catggcatcc gacgtgccga ctattgccta
      781 tgtcacgtcg cgggcctggt cggccggggc cctcatcgcc ctgtcgtgcc gccatatcat
      841 catggcgtcg ggcagcagca tcggcgctgc cgaaccgatt cccaatacgg agaagaacat
      901 tgccgccctg aaagccgagt tcagcgcgac ggcggcccag aatggccata atcccggcgt
      961 cgccgaagcc atggtcgaca agtcccaggg ctatcccgac tatgccgaac cggggaagat
     1021 actggcctta tccgacggcc aggccaggga actcaacgtg tccgacggct cggccgatac
     1081 gttgtccgaa gccctggccc tgtacagctt gggcgatgcc cgcctgacct atgtcgacaa
     1141 ggactggcgt gatgacgccg tcggcatctt gcagaatccc tatgtccgca cggtcttcgt
     1201 cgccctgatc atcgccgcct tgctggcaga aatcaagatg gccggcatcg gcgccggtat
     1261 tgctacggcc ttcgtcttcg gcggcctgct gctcctgtcg ggcaacgagt ccctggccga
     1321 cggcctgaaa atcgtcgccg ccttcctggt tagtctgctc tgcatcggcc tggaactggc
     1381 gtcgcctggc gtcggcatct ttggcctggc cggcgtcgtc ctcttgttcg gcagcctgtt
     1441 cttcatgctg ggagccacgt atgaggccgt ctacatcctg gccggcggga cggtcctggc
     1501 catcatattg ttctatatcg tcggcaagca tttgcccaag agccggcttt ttgaaaaatt
     1561 gaccctcaag aaccgttcga cgaaagaaaa gggatatacg gcccaggccg attacagcaa
     1621 atatctctat gaacggggca agaccattac cattctgcgt ccgtccggga cgatccgcat
     1681 cggcaaagag cgcgtcgatg ccacggccaa cggcacgttc atcggccgcg acgtcatcgt
     1741 ccgcgtggtc aaagtcgaag gcagccgcgt cgtcgtcgaa ccggaaccgg aacgccatcc
     1801 caaaccctga gaaggaggta tcttatgtct atcgtatcct ttttggcagt gcctgtcatc
     1861 atcatcgtgg ccgctgtcat catcgtatcc ctgttcctgc acttcgtgcc catcggtttg
     1921 tggatttcgg ccatggccgc cggcgtatcc gtcggcatcg tcaacctcat cggcatgcgc
     1981 ctgcgccggg tcatcccgtc gaagatcatc ctgcccctgg tcaaggccaa caaggccggc
     2041 ctcgatgtca acgtcaacca gctggaagcc cattacctgg ccggcggcga cgtcgaccgc
     2101 gtcgtcgatg ccctcattgc cgcccatcgg gcgacgatgt ccctgacctt tgaacgggcc
     2161 tgcgccatcg acctggctgg ccgcgacgtc ctggaagccg tccagatgag cgtcaatccc
     2221 aaggtcatcg aaacgccgtt catctcggct gtcgcccaga acggcatcga actgaaagtc
     2281 cgggcccggg tcacggtccg cgccaacatc gaccgcctcg tcggcggtgc cggtgaagcg
     2341 acggtcatcg cccgcgtcgg cgaaggcatc gtcaccagtg tcggctcggc acgggaccat
     2401 tcgctggtcc tggaaaaccc tgatgaaatc agtaagaccg tcctcagcaa gggcctcgac
     2461 gccgggacgg cctttgaaat cttgtccatc gatattgccg acgtcgacgt aggccgcaat
     2521 atcggcgccc gcctgcagac ggaccaggcc gaagcggaca agcagatcgc ccaggccaag
     2581 gccgaagaac ggcgggccat ggctgtggcc aaagagcagg aaatgcaggc ctatacccag
     2641 gaaatgcagg ccaaagtcgt cgaagcccag gccgaagtcc cgttggctct ggcccaggcc
     2701 ctgcgcgaag gcaagttagg cgtcatggac tattataata tgaagaatat catggccgac
     2761 acggacatgc gcgccgctgt cgcccaggcc ggcgtcccca agacccagtc ggtcccgacg
     2821 gggcaggaca gtcaccacga cgaccgcggt ccggcaggcc atagtgaaaa gtaggtgagc
     2881 cttatggata tgttatggct catcgtggcc ttcgctctca tggcgatcct gccggacctg
     2941 ctgcggcgtc gccgccgcta tcccaggcgg aaagggccga ttcccgtgcc gccgcggcgt
     3001 aaacccgcac cggccaaggc gccggctccg gctgaaaccg gggaagatgc ggctgggaac
     3061 gagatttccg cacaggaaac gacagctccg gcacagccgc ccatcgtgcc ggctgccgtg
     3121 ccggcccgtc cggctatagc tcccgtatcg gtgccgcacc gcgtccggcc cgcggcctgg
     3181 agccagctga cgccgcaggc gcgggagctc tacgccggct tcgtctggtc ggagatatgg
     3241 caggaaccgc tggcttaccg ccggcgatta attaaaaaat agagaggatt tatggaaatg
     3301 attcaagaaa aacaagtagg tatcaagggt atccccggtc tggtcattac gtccggtgaa
     3361 aaagcgaaag gcgtcgttct gttctatcat ggctggtcgt cgcagataga attccaggcc
     3421 gtccgcggcc gcatcctggc ttcctatggc tacgacgtcg tcattcccga cgctgtcaac
     3481 catggccacc gcggcacgct ggactatgaa tcgaaagtcg tataccccga tttctggcag
     3541 accatcttcc agaacctgac ggaagtgccg gtcatgctgg aatatataca ggaaaactgg
     3601 cccgatacgc ccatcgccgt catgggtcat tccatgggcg gcttcacggc cttaggggct
     3661 gtgagccttt tcagggaatt caagacggct gtcgtcatga acggctccgg ttggtgggat
     3721 gaatcggacc gccgcttccg ggcagccctg cacatcgaaa agccgcgggc ctaccgtttc
     3781 atacagatgc agttcgccgc catggatccc tatacgcata tggaccgcct cagcggccgg
     3841 tccctcttgt ccctccgcgg cggtgccgat gatgtcgtcg acggggctgc ccaggaactc
     3901 tatctggaaa aactggccca gcgcgatgac gtcaagaccc agtccatcgt ctacgatgat
     3961 ctcggtcatt tcgtcacgac caacatgatg ggcgatgccg tcaactggct ccaggcagaa
     4021 ctccattaaa gaaaaactga ggtttgctta aaaataataa gaggtaaaac ctttttctgt
     4081 ggaaaatatt aaaataatta tcgacataca ctttacaaat ttaaaaatac gagtataatg
     4141 tatatataaa cagaaaccgc aagggcgcgg aaacgtaaga atcagtaatc tttctgatag
     4201 aaatgattcg tttccaccgt tgcggttttt atatttttat tgttatcatt tataatcagg
     4261 ggatgtataa caggagggat aggtatgtca aagaatattt tccgaacgaa aagcatcaac
     4321 cagttcctgt cggaaacgaa acaggacggc ggcatgaacc gcgtcctggg gactttctgc
     4381 ctgaccatgc tgggcatcgg ctgtatcgtc ggcacaggga tcttcgtcct gaccggcatt
     4441 gccgccgcca attactccgg cccggccctg gtcatttcct tcatcatcgc cgcactggcc
     4501 tgcggctgtg ctgcactttg ctattctgaa ttcgccgcca tgattcccgt agccggtagc
     4561 gcctatacgt acggctacgt agccttaggc gaattttggg cctgggtcat cggctgggat
     4621 ttgattttgg aatacaccct ggccttgagc gccgtgtcca tcggctggtc cggctatttc
     4681 ggcaatatcc tgaccaatct gggattggct ctgccgaaag aattcattac ggcgccggaa
     4741 gaagggggcc tcatcaatct gccggctatg gccatcatct ggatcatcac cttgatcaac
     4801 atgaagggca tcacccagag ctccctggtc aatgacatca tcgtcgtcat caagctggct
     4861 gtcgtcggcc tgttcatcgc cctcggcatc agccacgtcg aaccggccaa ctggacgccg
     4921 ttcatgcctt atggctggtc cggtgtcttc acaggcgctt cggtcatctt ctttgcttac
     4981 atcggcttcg acgccgtctc gacggctgct gaagaagtca agaacccgca gaaagatttg
     5041 cctcggggca tcatcttatc gctcatcatc tgcacgattc tctacattgc cgtatcggcc
     5101 atcctgaccg gcatggtccc gtacttacag ttcaagacga cggccgctcc tgttgcctat
     5161 gcactgcagc tcgtcggcta tcattggggt gccgctgccg tctccgtcgg cgctatctgc
     5221 ggcctcacgt cggtccttct ggtcatgagc ctcggccaga gccgtatcct cttcgtcatg
     5281 tcccgcgacg gcctgctgcc gcgcttcttc ggtcacatca ataagacgac caagacgccg
     5341 gtccgcagct ccttgttggt cgccatcgtg tcgtccatcc tggccggcct cgtacctatc
     5401 ggcatcgtcg ctgaaatggt caacatcggc acgctggcag ccttcatcat cgtatcggca
     5461 tcggtcatcg tcctccgcaa aaaagcgccg gaccgcgttc gcccgttccg ctgcccctgc
     5521 gtaccgctgg taccgatcct gtcgatcctc ttctgcggta tcctggtcat catgctgccg
     5581 acagtcacgc agatccgctt catcgtatgg ctggccatcg gcctggtcat ctacttcggc
     5641 tatggctata agcacagcat catcaccaaa gcccagaaag gcacgctgaa cgcagcccct
     5701 gcggccgctg ctgatgaaac agccagagta ccttcggtcc acgaaggcgc tcactccact
     5761 attgaacact gacgcattct atatatcgac agaaaaagac ccgccctctc ccctggccgg
     5821 gtctttttct tgccgtcagc tgccagccgc ccgccctttg ggcgtcccct acgcacaagg
     5881 ccgccggccg tgtgtctttt gcggcctgcc atctgcggac tgcggcctgc gctcatttcc
     5941 atcgcctgca tgcgatttcg taggggccgt cccaaagggc ggcccgcttc aggataggtg
     6001 tggtttcccc agctagcccg ccaggtaccg accagcggga ggggaagggg gccagaacgg
     6061 acagccgtgg ctggcggccc gcaggcttct tgttaaaaag caacctctca gtcagctgcg
     6121 ctgacagctc ccctagcagg gg
//
LOCUS       DAWABZ010000109         6126 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_3414_length_6126_cov_3.165047, whole genome shotgun sequence.
ACCESSION   DAWABZ010000109 DAWABZ010000000
VERSION     DAWABZ010000109.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6126)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3414_length_6126_cov_3.165047"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1194 a         1642 c         1961 g         1329 t
ORIGIN      
        1 cgaccagggc gtaaacttcc ttattggcaa aggccgcctc ttccttgcgg aacagggccg
       61 tcagcctgtt gaagaaggcc gtgatgttgg cgctgacggc gtcgttgtag ttcgggcaga
      121 ccaggacgat gacgtcgccc tggcgcacag cgggatagac tttttcgacc atgacgccgc
      181 cgtagaagca gtcccctttt tcgccgaagt ggaggcacgt ttcgtagctg cagccccggc
      241 agtcgacgac gctgccgttg cgcagggaaa tctccgtgac cttcgccttc ccctgcagat
      301 ggcagcggac catctcccag aggaggagcg tattcgacgt gcggcggctg ctggcgtgga
      361 tgacgacgat atctttgacc ttccctgacc cggacgggcg aacgaaggac agcagcttgt
      421 tgaccaggtg gccggcactg acggcatagg cctcttcatt agagaggccc tggatcttgg
      481 cctgggtatt aaaattatag agagagcccg tagcctcgac gagaggcttc ccgggaaaga
      541 tacagccggc ctgattggca aagaaaatca gctcccggcc tatttttttt gtaaaaaatt
      601 ccccctgccc gtcgacgagg acggcaccgc ggcagccggc caggcagtga cggtggcgga
      661 ccaggcagga tacgaggtgg acataggaca ggctgacgcc ggccctgggc agggcgacgg
      721 caaagaggac ggctgtcccc tctttttccg ccggcgtgcg gacgcaccag cgcaaaaagg
      781 ccttttcccg tttgatgacg gtatacgtcc acccggccag ggcccggtcg aggacgcgcc
      841 ggattcgttc cgtgtggccc gcttcctccc agccgacctt gacgaccaac aacatggcaa
      901 ggcccccttc ctatacgagg cggctcagtg ccgtataagc ccgtttccag cgcgcataga
      961 gggccgggca ggcacagacg tcttcatagg tcacgcagcc gccggcgacg cggttcttga
     1021 cgccgaagaa agcgcctttc gatgtatcgc cgaggtagac cgacccgtaa tgccactggc
     1081 cgcgcagggt caggatgatc gacaaggccg ccgacgacag ggccggggcg atgtagggct
     1141 tgtagcccag gtcgcggacg gcgacgttgg catggacgac gaggcccgtc agttccctgg
     1201 acagcgcgtc gtcatagccg tcgaggcggt cggcgatgac caggtctacc ccgtggggtc
     1261 caaaggcgcg gccttcttgc agataatggg cgaagcgcgg atcctttttc gcatagtagc
     1321 aggcccgggc atgcatgacg cccaggccgt agccctggat ctgcgacgga tggagaccgg
     1381 aagcggtcaa aaaggcgcgg cacaaggggt cgaccggatc cgatacgatg cagacctggc
     1441 cgccgaagcc ggcctggcgg gccagggccg cataatgggc gacgagactg cggttggctt
     1501 cgagctgggc catgcgcacg tcgccgccgg cgccgacagg cggtacgccc ttgctggcac
     1561 agaaaatgaa gacatcgcag tcgaagacgt ctttttccgg gacgatggtc acggccggca
     1621 ggaccgggtc gtcacagcca tccagtccgg caaaggggta gcgtatctgg ttcatctcca
     1681 tttccaggcg ggccaggttc ttctcgttga tatcgcacat gccgacagaa ccgatgacgt
     1741 cctggccggc caggagcagg ccgatgagca tggtcgtgcc cacatcgccc aaggcgacga
     1801 tatggacgcg ggccttcccg gccagctgcg gtgccctgtc gaggggcatg gcagaggctc
     1861 cgatttctcc gggcagatca tgtatccatt tattcaagtt ctccatctcc ttccccccag
     1921 cgcaggcgga tgagccgttc gatatcgttc ttgatgaagc gcgtcgggtc catgttctca
     1981 tcgaacttga cgccgacgtc gatatcgggg atgcgcgggt aggtaatgac ttcgccgagc
     2041 cgcttcaggg tcaggcgttt ttcaaaggtt tcctggtaga cgtcctgcaa tgtctccatg
     2101 atagatgcgg cattttgcag acacgtcagg gagtataagt agtcggcgtc gctgttgccc
     2161 ttgccgatga aagccggctt gacatagggc aggaccaggt tgatcgacgg attgcggccg
     2221 tagccgctgt agttatgggc attgtccagg ctgtcgccgc cgatgaaggg atagcagtcc
     2281 ctttcgttga gccagacttc caggctgggc aggaaataag cgctctcggc gcggatgtcc
     2341 ttttcttcca tcatgtccat ggcctgaccg gtcatgacgc cgaccaggac ttccttgatt
     2401 tcgacgccgt tctgttccag gtagggcgtc agcatgcgca tgcggtggcc tttgtgcagg
     2461 aggtcgtcga tgaggatgac cggcctgtcg aaggacttga tggtcttgac ctgattgtcg
     2521 acggacgaat agtgctgaca ttcggcgatg gtaaaagact tgaccgtgcg gtcgaaatac
     2581 ttgtcgatgt gcaggccctt ggtcaccgtg ttgggtacga ggacattgct cagggccttg
     2641 ccaaagggaa cggtcatgta cgggccgcgg cgcttcttct tgtcttcgac gatggaaacg
     2701 ccgttgattt cagcgacttt cttgatgatc ttattataaa aggcactcat gttgaaagac
     2761 aggatgagct ggcccggata aatgtggttc atgaccgaca acagattgtt gtgggcctcg
     2821 tagatggccc ggcggacgcg gggattcttg ttgaaggggt tcttgatggt cgtttcgacg
     2881 tcgcggaaaa tgacgatggg cgatttcatg ttgactgcat aaatcggcgt ctcttccggc
     2941 ggcgcaatgt tgacgaaccc ctggcggcgc aatgcgtcga tgacgggctg ctcgcagccg
     3001 gccggatcga cgggatggta gacggcgtag gcgtagtcgc gggcaatgag ctctgtcagg
     3061 atttccgtaa tgatgatctg gctgacattg gcaatgccgg ccatgtcgtc ggcatagaga
     3121 aagccgatgc aggcaatgcc tccgtcagcg gcatcgcgga tgtgcgaggc aatggccgag
     3181 tcgtggaact cgcgcagcaa atcgcgggag cccacgcggt ggacggcggc gtaagcgatc
     3241 atcttgcgct cctggctggc gtcgtcgatg tacagcgtcc agacatagtc cttttcgacg
     3301 tagctgtcga cggtctccgt atggtagcct ttatcccaga gggcgctgca gatgggccac
     3361 aggctttcgg cgccgcgcgg cttgaaagag ccgatgccga tttcccgagc ttccaggacg
     3421 tgcttgtacg ccggttcgcg caggtaaagg ttgttatcgt aaatgaagtt ctgtgccacg
     3481 gcgtcgatga ggttggaaat atcccggtta aggtcgatgt tctcgcgtat cttcgtcgag
     3541 ctgatgtctt cgtagaactt gtccagcgtc aggcggatga ccttggcctg gatgccgtag
     3601 gtcttttcat gctggtccgg ttcggcattt tcccgcgtct ccctggcgaa ggcgatatgg
     3661 ttgaccgtat ggatcgaatc cggcgtcggt acggcccggt aagccgacgc attttcgatg
     3721 acgtccgtgc cgacggcgat gtacaggtcc cggccggcaa agatgtcctt gagcttcttg
     3781 atgtccgccg gattggcgat gttgatgggg atatcgtccg ggaagggata catgttctct
     3841 tcatcggcga cggatatgtt catgatcttg cggcgcatga gccgcggctg ggtgtgcttg
     3901 gaccaggaaa attcatcgag ggccaggtaa atgtcgaagc ccaggtcgcg tatcttgcag
     3961 gccacggcct tgtggcccag gctgaagggg tcgaaggtcc ccggatagaa gcagggcttg
     4021 cggcctgttt caaaggtaaa gggccctctg gcgaattcgt gctggctgat gtagcggtaa
     4081 atatggttga gcacggccga attactgtag aaatccaggt tttcttcgct gttctcatcg
     4141 aggaggacca ggagtttctt atagcagtgg ctgaagagga aatctttccg ttcgtccggc
     4201 atgagcgggc tgtggaagat gaatttccct aaatcgcgaa aggcgtcgcg gctcagttcc
     4261 ttattgtaat gggcatgggc cttgatcatg atgtacagca ggcggcgctg gcgcttttcg
     4321 ttgaccgccg ggtcttctga gaactctttg gcaaattcgt cgaattgttc caaaatgatg
     4381 cccactgtct ggaccatgga cgctgccaag gggatgttga cagtcagcag ctggccttcg
     4441 atggtcttga cgaattcgtc gaactcccgc ggcggcagct tgaggatcat cttgcccaaa
     4501 aattccggca cgtatttaga aatctgcggg tcgccgattt ccaggccgtt gaacagttcg
     4561 accgtcagtt cgttgcgctg ggcataagtc atgttcgagg caatcttgag caggctgttg
     4621 ccggcggcgc ggcggacgaa gatggattcg ctgaccttga gcagattggt cagatgggtc
     4681 ccgagatgca tgacgctgcc ggcatcgctc tggccgtcga tgtagcgcag catgagttcc
     4741 aggttggcca ctttgacgac ccaatgggtc cccatcttga ggtcggccag gaagaggttg
     4801 ccttcccgtt caaagaaggc gtcccgccct tcaggcaggt tcatgacctt gagcaggtcc
     4861 ttatagtaca agtcatccgt atagtcggca aagacgtggc gggcgcagtt cagggcggcc
     4921 acgcgcaggt tgaaggattc actctgcttg gccctttcta tgaaggggcc gacggcggcc
     4981 ataaaggctt cgtccagata gtcccggtcc atgtcggcca ggaccttcaa gaggatgata
     5041 tactcttctt cactgtaatt gcgccgttca taatacggcc ccatgacggc cgtatagcgg
     5101 ggacgctggg accgggcaca gcggtgcagg acggccgata caaaggtgct caggcagtag
     5161 ccgatccaca gcttgtgctg ctcggtgaag cggatatccg ggcagatgat gcggtccagg
     5221 taggtcgtga acagggacag gttggtcacg gccttggccg gcagggagac gccttcgggc
     5281 agttccttgg tatacgtatc gttgaaattg gcgatgatgc gtcccatgag ggctgccgcc
     5341 tgcaggcgga tgtcgccttc gcggtgggac aagagctcgt agaggaactt cagggtcatg
     5401 agcttctgat gctccgtcat gtacgtcgaa tattcctcga agatgccgat atacgtgcgg
     5461 acattcttcc actgccgctc gctgcgggcc gcttcgatga ggttgccaaa atcgctttca
     5521 tcacggaaga tgctcatgag gcggatgttg tggtcgatgg cggcatattt gagctgggca
     5581 atgacgtcat tgccttcgag gaggactttt tcccgatgga tgggctgcgg cgggacggcc
     5641 ggttcccagg cgaagtcggc aggcagttcg gtgacggcgc cgtgttcttt catgaaatct
     5701 tcaaagtccg ccaatttagc atagactttc tgatagcgct ggcgcttggc gtcgtcgaca
     5761 ttatccagct tccccaggat gacgtcgaag gcctgggcca gggaatagaa atggacgatt
     5821 tcccttccct gggcatcgcg gctgctcttg acgcggaaat cggcataaat gagcaacagc
     5881 gattcgacgg acaggttttc cagttccagg tcccagacgg aatggttagc cgcgatgtgg
     5941 ccgatacccg gcaggcccag gcggcggcag cacatgcccg tataataata atgtaaataa
     6001 gggacccgct tctcttcatt cttcttgcag ccgtacttgc cgatatcatg gcaggcagcg
     6061 gcgccggaaa tgagggcgac gtcgacgggg atgccgttcc gcgccagctg gcgagctgcg
     6121 taaacg
//
LOCUS       DAWABZ010000110         5870 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_3561_length_5870_cov_3.000000, whole genome shotgun sequence.
ACCESSION   DAWABZ010000110 DAWABZ010000000
VERSION     DAWABZ010000110.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5870)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5870)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5870
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3561_length_5870_cov_3.000000"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1171 a         1661 c         1745 g         1293 t
ORIGIN      
        1 caaaatgtaa acgatgttat tcttgacaac gtgcgagccc ctacaaacgt cttacagcga
       61 gcacctacaa atgccctcca ggcacctctc ttatcaggaa aggccagtta cgaaccacta
      121 gccacgaata atctcgctcc aggcgttggc gatgtcggcg aattcctgga gagacagtgt
      181 ttccccgcgg cgtttgccgt cgatgcctgt tttttccagg acctgggcga tttgttcctt
      241 gctcatgccc gtgtttttca aggcgttgga aaaggtcttg cggcgctgct ggaaggccgc
      301 tttgacgacc tggaagaagc gcttttcgtc gattaaatcg acaggcggct gctggcggac
      361 gtccatggcg acgacggccg acgtgacttc cggtgccggc atgaagcatt tcggcgagat
      421 ttcaaagagc atctgcggct gcgtatagta ctggaccgac accgacagag ccccgtaaat
      481 cttatggccc ggttcggcta ccatgcgttc ggccacttcc ttctggacca tgacgacgat
      541 gcgcttgatg ggtagcttct gttccaggag cttcatgatg atcggcgtcg tgatatagta
      601 gggcagattg gcacagaccg tgaagggctg gtgattcatg acttcttcaa tgtcgatgcg
      661 caggatgtcg ccgtggacga cgcgcacgtt gtcatagtgt tccaaggtct tgtcgaggac
      721 gggcaacaga tggtcgtcca gttcgacagc cgtgacggct gcgcccgttt cggccagggc
      781 ctgggtcagg gtgccgatgc ccggcccgat ttccaggacc ggatcgccct ccccgatttc
      841 ggcagcgtcg gcaatgtcgt cgacgacggc gtgacggatg agaaaattct ggcccagttt
      901 cttattcatg cgcaggccaa agcgtttgac gacgtagcgg acgacgtcgg gattggacaa
      961 gtcgacttct ttcatttatt tcacctcgtg gatctgacgg agggcatcat cccattcgtc
     1021 acgggtgacg ccgtaatggt tcaagcgttt caaaaactgt ttggcattgc catagccgat
     1081 acccaggatg gcgccgactt cggcccggcg gtcggccgca tcaggcgtcc cgttgaggcc
     1141 agcagcggaa atgtcctgca tggtaaaggt cgcctgcggt tcatacgtcg cacagcggac
     1201 tttggacaag gccaggcgga tggcttccgg cgaggcctgt tcgacgccca ggtcgttgtt
     1261 ggcaatggca tctttcctgg ggataaaggc gtgtttggca ttagggaagc ggtcggtcag
     1321 gaacttgcgg atctgttcgc cggcccggtc ggggtcggtc aggatgatga tgccccgctt
     1381 ttcataagca cagcggatct gttccagcgt gtgaggcttc aaattgaaac catctgtcgt
     1441 aatcatatcg acgtcgatgt gactggcccg gatgcgggcg acatcggacc ggccttcgac
     1501 gacgaggact tccttaatca tgagcggctc cttcccggtg ttcggcaatg tactgggctg
     1561 cgctgaggat ggccaggaca ttgtgttcaa agacgatgcc gccttggaga taaatcatgt
     1621 acgggtcgcg gatcgggccg tcggccgaca attcgataga cgaaccctga acgaaatcac
     1681 cggcggccat gatgatctta tcggcatagc ccggcatatc gtcgggaatg ggtgtgacgt
     1741 gggcgtcgac aggcgagaag gactggatac cctggcagaa gagacacatg ttctgttcgt
     1801 tttccaggta aatggtctgg atcaggtcga cgcgcgggtc ctggacggcc ggcgagacct
     1861 tgaagcccat cttttcaaag acagccgctg caaagacggc ccctttgata gcctgagccg
     1921 taacgtgcgg tgccatgaag aagccctgga aaaagaggcg gtagccggct gcataggaac
     1981 cgagttcgtc gcccaggccc ggagccgtca ggtagtcggc tgcggcgtgg accagttctt
     2041 ccttgccggc aatatagccg cctgtgggag caatgccgcc gccgggattc tttatgagcg
     2101 acccggcgat gatgtcggca ccggcttcaa tcggttcttc ttcagcagca aattcgccat
     2161 aacagttgtc gacgaagcaa atcgtcttgg ggctgacctt cttgacgacg gaaataatag
     2221 aacggatatc atcaatcgtc aaggatgccc gcgtgctgta accgcgggaa cgctggatga
     2281 ggaccagttt cggctgcttt tcggcgacgg ccgcttcaat gccggccaaa tcataggcgt
     2341 tgtccttcaa ggggacttcc tcatagcgga cgcccttttc cttcaaagag tgcggcgttt
     2401 catgggcata gccgatgacg ccctgcatcg tgtcataagg cgccccgacg aggctcatca
     2461 tgagatcgcc ggtctgcaac agggccagca ggaccgtcgc cagcgcatgg gtccccgata
     2521 cgaagtgggg ccggacaatg gccttttcgg ccttgaagac gcgggcaaag acttcatcga
     2581 gcttttcccg gcccatgtcg ctgtagccat agcccgacgt accggcaaac tggtacgaag
     2641 aaatctgggc ttccttataa gcggccagga ctttcgccgt attatggaga caaatcgtat
     2701 caatgcgttc aaactggtcc gcacattcgg ccagcgcttc ttttcgtaat gtttctaaat
     2761 ccaaagacaa tgtcatgatt cctttctata atgaatgtat aagttagtgg ctagtggtta
     2821 gtgaatagta gttaggggtt aggggctagt ggctctcctc ataggagagc tgtcagctct
     2881 gctgactgag aggttcttcc agacaatgag acgctatacc gttcaggacc ttttttcatt
     2941 tcgggccgcc ctccgggacg gcccctacgg agattgcttt tttccttaca gtccgctgtt
     3001 tcgcaggtcg caggtcgctg aaggaaacct ctccgcctta cggcacctcc cctatcaggg
     3061 gaggctttcg gctctcctca taggagagct gtcagctgcg ggccgccctc tgggacggcc
     3121 cctacggagg ttgcttttct acttgccgcc tgcgccttcc cgtcatggga aaggcacatc
     3181 ccccagcaca gcctgtatca atgcttcttc tccatcgtac tggtccttgt cgtaccagtg
     3241 gatatagggc atgcgtttga accacgtgag ctggcgtttg gcaaagcgcc gcgtccgggt
     3301 cttgatgcgg gctgcggcgt catcgagggg catctgtccc tggagataca gggccagttc
     3361 ctgatagccg atggccttca tggactgaca gcccggcggg atgcccttgt ccagcaggta
     3421 ccgcacttca tcaacccagc cggcggcaat catggcgtcg acgcgctctt caatgcgccg
     3481 gtacaagaca tcccgcggca ggcgcagacc gatgacgtaa gcgtcataga ctacatcccg
     3541 agccttgccg gcccggacga aagattttcc ttcgccctgc tcgatggcgc tgaccaggcg
     3601 ggtcaggcgg tgcgtgttgg aaaacagctc gtgccagtcc ggcggctgcc agtccgtttc
     3661 tgtctcgata taggccttca gttcctcttg actgagagcg tcgatccgcg cctgggccgc
     3721 ttccttgtcg gcttcggtca tgcgggccgt ctggaaatca tagccttcca ggagggactg
     3781 gacgtatagg cccgtaccgc cgacgagaat gggtatcttc ccctttccgt tgacttcggc
     3841 aatggccttt cccgccatat cacagaagcg ggctgccgaa tagggttcgt cgggctcagc
     3901 aatatcgatg agataatggt gatagcgggc cagttcgtcc gccgtcggct tggccgtacc
     3961 gatatccata tggcggtaaa tctggtaggc gtcgccggaa atgaggtcgg tcccgtagtg
     4021 ttcggccagg gcaaaactga gggccgtctt gccgaccgcc gtcggaccga tgatggcaat
     4081 tactttttcc atgaatcaca ctccaatacg gcatagccaa tgcggctgta tttgccgcca
     4141 tataaggcaa catccgggaa atcgcggcag agctggcgga aatcccgttc tttgatgacc
     4201 acccggcgcc gggctttctg cagcgcccgt tcgatgaggt cccgcgtcag accgccgtgt
     4261 tccatgatgg cccggacggg ctggaactgg cagctcgcct cgacaggccg ctggaacatg
     4321 gggtcgaaat agaggacgtc gtacacaggc ccgtcatcgg ataagagata atcgcgatag
     4381 tcgccacagc gcagggaaat ccggcgcagc gccgccgtga cgtcgtcttt cttatggaca
     4441 aagtgggaaa agccccaggc cgtaacggct tccagcaaag gcgatacttc cagggcatcg
     4501 acgcgggagc cagccggcag accgaaagag gcgacggccg catcggcgcc aaaaccacag
     4561 gtacaatcga ggacggaaat gggcggctgt gccgctgcag gccgcaggtc gtactccttc
     4621 acccccatca gggcttccag gagatggtcg cgctggcctt tccgcaggtt ctggatgcgc
     4681 agttcagcca tgttgaggct gaagaaatgg ctgccttccg ggcggtctat ctgaggcccc
     4741 cgtgaggaat agacgaggaa atcctcgccg tattcatcca tcaaatcctg tatggtccgc
     4801 tttccccgcg gcacataggc aaagccctgg ctttcggccc aggctttccc ttcacattgt
     4861 aatttttctt tggccttcaa tgaaggcacg acgtaaatca tatgagtcat gtccgtttaa
     4921 ataagtgcgc taattctttt ggcgtaaagc ggatgatcgt cggccggcca tgagggcaga
     4981 cgtagggttt ttccgtatgg aacaaatcat ccaggagctg cttcatctgg cggatattga
     5041 ggctgtctcc ggccttgacg gcaccgtggc aggacagata ggccagcgaa cgatggcgga
     5101 ccatggcttt atccggttcc ttctggtcat gcaggagcag gcagatatcc tttaaagaat
     5161 cggcaatatc gctggtctgc aaatcgcagg gcacttcttc gacgcgcagc gtcgtcggcc
     5221 cggcttcgga ataggtatag cccaggtcgt tgaagacatc ctgccgttcc aataagagcg
     5281 tcatatcctc gttgtcgact tcgacgaact ccggcgtcag caactgctgg ctcggcatcc
     5341 gttcgacacg cttgcagaac gtatcgtagc ggatccgttc atgagccgcg tgctgatcga
     5401 cgatgtataa atcctgaccc ttcttggcaa tgatgaagca gtccgccact tcccctaagg
     5461 gaatgaagac gtcgtcatcg ccgtcaaaga cgattttagg cgctgacgcc tcggaaacga
     5521 ccgacgtcgc ttccgacggg ccgccctggc catgctgggc caaagcctgg gaaaacaagg
     5581 aagaaccatc cgcaagaggc tcccctgata ggggagctgt ccgcgtagcg gactgagagg
     5641 ttgccttttc ccattgcggg ccgccctttg ggacggcccc tacggaggtt gcttggccac
     5701 tttcctgcga gcgggcgccc cacgtgggcg cccctacatg ggcttgctgc gggccgccct
     5761 ttgggacggc ccctacagag gttgcctggc cactctcctg cgagcgggcg ccccacgtgg
     5821 gcgcccctac atgggcctgc tgcgggccgc cctttgggac ggcccctacg
//
LOCUS       DAWABZ010000111         5846 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_3582_length_5846_cov_3.227422, whole genome shotgun sequence.
ACCESSION   DAWABZ010000111 DAWABZ010000000
VERSION     DAWABZ010000111.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5846)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5846
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3582_length_5846_cov_3.227422"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1241 a         1590 c         1609 g         1406 t
ORIGIN      
        1 tttgcgaacg gctaacagct agctgctgac tgccaaaaag gcgctttcgc atgggcgaca
       61 gcgccttttt cactacaaac ttgatactgc aaactatatt ctatttatcg atgacttcct
      121 tgacgatgct gatagcttct ttcaattcat cccaggggat gttgagcggc ggcaggaggc
      181 gcaggaggcc gttggccgtg aggacgagga cgccgtggtc gacgcagcct tctacgacgt
      241 ccatgagggc tttattcttg agggtgacgc caatcatgag gcccatgccg gatacgtggt
      301 cgacgtgttc gcactgttcc agttcttctt tgatgtattt cccttttttc cggacttctt
      361 ccatgagggc gtcgtcgagg cggtcgatga tggaaatgcc gccggccgtg caaatcgggt
      421 tgccgccaaa agtcgaacca tgggtatggt tggtcagggt atgctgcgtc gaatcggcga
      481 acatcgtagc ccccaagggc aggccgccgc cgatggcttt agccgtcgaa atgatatcgg
      541 gctggatgcc gtagcccatg taggaataga gatagcccgt ccggccatta ccggtctgga
      601 cttcgtcgac gataatcagg atatcgtggg catgcgcata ttctgcgacc tgctggacat
      661 attctttgtc gaggggatgg acgccgcctt cgccctggac catttccatc atgatggcgc
      721 agacgttatt ttcttcgcag agccgcttca tatcggcaaa atcatcgggt tcggcataga
      781 cgaagccgtc gacgaagggc ccgaagtcct catggagcga atcctggccg cacgccgtca
      841 aggtggcaat ggtccggccg tggaagctgt tcttcaaggt gacgatggta tagcggccgt
      901 cgccgtattt gtcgtgcgaa tacttgcggg ccgtcttgat ggcacattcg ttggcttcgg
      961 caccggagtt cgagaagaag accttcttca tacccgtctt ctggcagagt ttttcggcca
     1021 ggacggtctg gggttccgta aagaagacgt tggaaatatg gctcaattta tggagctgcg
     1081 cttcgacggc tttgatccac acgtcgtcac agacgccgaa gatgttgacg gcaataccgg
     1141 cgctgaggtc gatgtatttc ttgccgtcca catcatacaa aattgcgcct ttgccgtggt
     1201 ccaatacggc accgacccgg ttgtatacat tgacgatata ttctttatca agggttttat
     1261 aatcttctgc cattagggga ttccttcttt cttataatat gggagcctga cggctccgca
     1321 ggccgctcaa agagaccgtt tttcgttcgt agttcatcgt tcatcgcgat ggcttggtat
     1381 ttttaactcc catacgatga acgaggaacg acctacgacc aacgaaaaaa atttattttc
     1441 atgtgctagt cgcatttatt tgccgattga atagtgccct acgaacatgg tgccaagacc
     1501 ctcttcggtg agcatttcga tgaggatggc atggggaacc gtgccgttga tgatgaacac
     1561 gcgcttgacg ccggccttga tagcatcggt gcagcactgg actttgggaa tcatgccgcc
     1621 ggcgatgatt ccgtcgtttt ccagctgccg cgcctggtcg agggtgattt ccgtgagcaa
     1681 tgaatcggga ttgtccttat ctttgaggac accgtcgata ttggtcatgg acagcataca
     1741 ttctgcattg agggcaccgg caatcttggc cgctgccgtg tcggcgttga tgttataggc
     1801 ctggccgttg gtgtcggtcc caatggtcga aatgaccggg atatagcctt tatccaggtt
     1861 atctttgatg atggaaacgt cgacgtcggt aatgcggccg acgtagccca gttcatcgct
     1921 ctgtttctgg actttgatca tctggtcatc gatgccgcag aggccgatgg ctttgccgcc
     1981 gatggtcccg atgaggttga ccaggtcttt gttgacctga ccggccagga ccatctggac
     2041 gactttcatc gtttctgcgt cggtgacgcg caggccgttg aggaatttcg attccatgcc
     2101 caatttattg agcgtctctt tgatgtgcgg gccgccgccg tggacgagga cgaccttgac
     2161 gccgatgagg ttcaagagga cgatatcgcc catgaccgat tttctcaggt cttcgttgat
     2221 catggcattg ccgccgtatt tgacgacgac gattttgttg ctgtatttct tgatgtacgg
     2281 cagggcgttc atcaagatct gcgccttctg ggcattggtt acggatttga catcttccga
     2341 gacgagttcg attcgtttaa acattacaat agattccccc attaactgca ggccttcaag
     2401 gaattttgga agcctaattt tttcaataac tgttccagtc catcagccag tacctcctga
     2461 ctgcacgggc tgctgccggc catgccgttg gccatgacga tgaacgtaaa gttttccgtg
     2521 tcgatggccg ccttggcctg agccagggcg ccttcgatga cagcactgcc ggcctgggca
     2581 tcggtcatga tgacacataa gccgtcgctg tcggccgtgc cgtcggcggc gacaccgctc
     2641 agagaacagc cgggcgtccc actggacaag gctacctgat aggccgtgac gtcgccggca
     2701 ttatccagaa ccgcttccag cggctggctg tcggtcccca atcctgccgt cagcgtcttg
     2761 aggctgtgga tgagcaggga cgggcggaaa tactggcctt tggccccgct ggcgaccagg
     2821 gcgatatcgt ctggcgcttc atccatgcat ttcccggcag cctggcagac ttcctgagaa
     2881 atctcatggc cccgcggccc atcaggaagg gccgtaccgt tgtgcaggag cagtacctta
     2941 ccggactgag tcatcaggcc ctgactcgtg gcggtaacga tggagcagtc gcgtcggctt
     3001 acgatcatgg cgacatccaa tttcacacgg ctgctgcata atccactgta cgtaccggca
     3061 gcctgaaatc cttgcggtac gctgaggcta tcataaacct gactcatact gactccacct
     3121 ttcatctgat tcatctaata ctttaaattc agccccgtcg tgggatcaat acccatcatg
     3181 aggttcatat tctggatagc cgcaccggaa gcgcctttgc cgaggttgtc gaacaaggac
     3241 gtgaccatga tgcgctggtc attgccggtg accaggattt ccatatcgtc gtagccgctc
     3301 atatcattgg ctgcgatgaa gccgcccttg tcttcgccaa aaggaaggac gtggacgaag
     3361 gggcagccgt cgtaatggct ggccaaaacc tgccagacgt cgtgagccgt cacctttttc
     3421 gccagcaagt ggctgaagaa aggaatcatg acttccatgc cactgtagta gtcgtcgacg
     3481 ataggcgtaa agaccggttc ctgcgtcaaa tggcagatgg tgacgatttc cggcaggtgc
     3541 ttgtgctgct gtaccaggcc gtactggcgc ggtgcatata aatcctgccc tttgtcgcct
     3601 tcgtactggg caatcatctt cttgccgccg ccgctgtacc cggtcaggga aaaagccgtc
     3661 accggataat cggccggaac gatgccggct tctgtcaggg gctgtaaaat ggaaatggcg
     3721 ccgctggcgt ggcaacccgg attgccgacg cggtcgctct tggcaatggc tgcggcctgc
     3781 tggggcgata attcggggaa accatagatc cagcccggtg ctacccgccg cccggtcgac
     3841 gtgtcgatga ggcggacatc acaaccttca gccagggtta cggcttcggc tgcggcaccg
     3901 tcagggagac aaaggaaggc gatgtcggcc tgtttcatgc aggcccggat ggcgtcggga
     3961 tccttgcgga catcgtcggc aatgggaacc aattcgatat cctggcgggt actgagccgt
     4021 tcatgaatcc gaaggcccgt cgtcccttca tgaccatcaa tgaatacttt cgttttcaca
     4081 cttcatcact cctatatcac atgggctgtg ccctgactat ttgttgaatt tttattcatt
     4141 atagatgcat tttaatattt tgtcaagaat tatgcacgat tttttacatg aatattagta
     4201 ggggctcgca cgtggcgagc ccgcctgcaa ttgaatacat ctcctttata tgaataaagg
     4261 gactacatgt aaacccatct ggcaagtagt gttcatcccc cctagttcag aagcgggcgc
     4321 cccgcgtggc gcccctacaa atcgcactca ggcgatgatt tatgttcgac atgcatatat
     4381 tacaacggcc gtagaccgca aacatcaata aaggtaagcg atgcgccctc cctatcggca
     4441 tcgaggcgga ctttgacgcc gtaaggcacg aaggccttgt ccttggtatg gccgacgggc
     4501 agtcctttga tggcgggttt cccggccaga tgggcataat aatccaggac ctggtccgtc
     4561 gtgaagtcgc ctgggtcgtt ggggcagtcg gtgaaatcgc cataggcgat ggcggcgacg
     4621 cgggacaaaa ggccgctctg ccagagctgg ttcagcatgc ggtcaatgcg gtaagcatct
     4681 tcgccagtat cctccaggac caggatacag cccgtcccgt cgagggcata aggcgtgccg
     4741 acaagggatg tgagaaccgt cagattgccg ccgacgatcg gcccttccgc ctgtcccgga
     4801 acgacggtcg tcagcttccg cccggccggc agggccagag ggcctcgcca tgcatctgtc
     4861 gtaaggccct ggaagaactg gcctgccgta taggcatcgg agccgccgag ggtgaccatg
     4921 agcggtccat tggctgtgac taagcggcat ttttcccaga gggcaatctg caaggccgtc
     4981 acatcgctga agccgacgac cagtttggga tgagacgcga tatattcata gtccagctta
     5041 tcgaggatac gcgccgagcc gtagccgcca ttgaggcaga taatggcctt gacatcatcg
     5101 tcgtggaaca aggcattgat atcgcgggcc cggtccgcat cggtcccgga aaagaaacca
     5161 taattcccag tgaccgtcgg cgtcatcttg acgcgataac cggcccgttc cagcctggct
     5221 acggccgcag aaatatcctg gccttcatcg tgcgtggccg gcgctgcgat gccgatgcag
     5281 tcaccgcggt gcagtgccgg tccccgaagg gccgttcccg ccgttactcc cccttgtaat
     5341 aacgctccat acccttggca tagccatagt cgtcattcag atattggccc gtcttctgct
     5401 tgtaacagag attgacggcg acgaaatcgg caatctgatt gtgcttgatt tccttcttgc
     5461 gcatagggat ttcctgccag ctatgggtcg aatcattata cagccaggcc tggccatgag
     5521 ccaccttata agtcagggta tacgtccctt caatattccc cgaatcctct ggcgaccgga
     5581 agagattgac cttaaaaatc cagccctgac cgtcatcctt cacggtttcc agcgaactca
     5641 aatcgacgta catttcataa cccatgccaa aattaatcgg ccggtaatga ctattcgcag
     5701 ccaaaggact gttgaaaaaa gcctgaccgc tgccaaaacc tccgagggcc accgcggccc
     5761 ctaatgatag aatcattaac ccacgtttca tattcatacc aaaaacctcc cttcaaagta
     5821 tgcctttatt atacgacaaa aaagag
//
LOCUS       DAWABZ010000112         5761 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_3634_length_5761_cov_2.515948, whole genome shotgun sequence.
ACCESSION   DAWABZ010000112 DAWABZ010000000
VERSION     DAWABZ010000112.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5761)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3634_length_5761_cov_2.515948"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1394 a         1494 c         1621 g         1252 t
ORIGIN      
        1 gccgaaacct tcatgcgcca gtcccccttt tcagtcgaac ttatttaccc atagcattga
       61 ctttggaagc caaacgggat tttttacgag ctgcagtgtt tttatggaga acgcctttct
      121 gtgcggcgct gtcgattacg ctgctagctt tatcgaatgc ttttttaaca gcttcagcgt
      181 caccagcggc gatagcttcg gtcgtacggc ggatagcggt atgaacagaa gatttaacct
      241 gagagttagc tgcgtggcga gctgcgtctt tttccatcgt tttgatattg gatttaatct
      301 gcggcaatgg attcacctcc ctaatttaca agaacactaa aagaaatttt accatatggc
      361 gcagaaaaaa gcaatacctt ttttacaaga catgcgatta agctttgcct tccgagccga
      421 ggacgcattt gatcttgtga gcgacgatgt catggacgaa ctgacggccg tcggcgaggt
      481 actggcgcgg gtcgaaatgt tccggatgct gtgccatgtg ctgacggatg ccggcagtca
      541 ggccgagacg gaggtcggag tcgatgttga tcttgcagac agccatggaa gctgctttgc
      601 ggagctggtc ttccgggatg ccgatagcgt cagccaggtt gccgccgttt tcgttgatga
      661 tcttgacgta ttccggtacg accgaagatg cgccgtggag gacgatcggg aagcccggca
      721 ggcgtttgga gacttcttcg aggatgtcga agcggagtgt cggcggtacg aggacgccgt
      781 cagcgttgcg cgtgcactgt tccggagtga atttgaaagc gccgtggctg gtgccgatag
      841 cgatggccag ggagtcgacg cccgtgcggg atacgaattc ttcgacttct tccggacgcg
      901 tgtagtgagc ttcgtgggca gcgactttga catcgtcttc gatgccggcc agctggccga
      961 gttcgccttc gacgacgacg ccgtgagcgt gtgcgtattc aacgactttt ttcgtgaggg
     1021 cgatattgtc ttcgaaagaa tgtttcgagc cgtcgatcat gacggaagtg aaaccgccgt
     1081 cgatgcagtc tttgcaggtt tcaaagtcag cgccgtggtc gaggtgaagg gcgatgggga
     1141 gatcatgatc ttccaaagct gcttctacga gatgtttcaa atacggcggt ttagcgtatt
     1201 tgcgggcacc ggccgaagcc tgaaggatga tcggcgagtt caattcagca gcggcctggg
     1261 taacgccctg gacgatttcc atgttgttga tgttgaatgc accgatagca tagtggcctt
     1321 cataggcttt tttaaacatt tctgtagttc caattaatgg catgtttaca ttcctcctac
     1381 ataatacgta aaaatggtta atatatataa ttccagccgg cccgtcggac gaaggcgtcc
     1441 atatcttcag gcagccggga attaacggtg atgaagcggt ggctcatggg gtgggtgaac
     1501 tggagacagt aggcgtgcag ggcctggcgg ccgatgaagc ggcgcgaacc gccgtagagg
     1561 tcgtcgccgg ccaggggata gcccaggtaa ctgctgtgga cccgtatctg atgggtccgg
     1621 cccgtgtgca ggacgacttg gagcaggctg tagtccttgc cggctgcgag gcaggtgaaa
     1681 tcggaccagg ccggcttgcc gtcgaggcgg acacagcggg tgatgatgct atcgggacat
     1741 cttccgatgg gggcgtgtac agttgtgagc cgcgacgggt atcgtccctc acagatagct
     1801 aagtacaagc gcttatagcc caagtcctgc ttatcgaaag cgtattggat ctgcggttcc
     1861 ttggcgacca tacagagacc ggacgtattc ttgtccaggc ggtgcatggg gtggtaggaa
     1921 cagtgctgcc ccgtctgttc gtaatgatag gcgacggcgt tggccagtgt cccgtcccgt
     1981 acggctgatg tgggatgcat caggagacct gcaggcttgt cgatgaccat gaggtagtcg
     2041 tcttcataga cgatgccaat gggtatgtcc tgtggcgggg aggggttctt ttccggaagg
     2101 gtgacgcaga tctcgtcgcc ttcatggacg aagtcccgcg tcgaggcggc ctggccgttg
     2161 atggtacaga tgccgttgtg cttgatacgg cgccgcaatg tcgacgaaac gccgaaatgt
     2221 ttcaaagcgc cgcctaacga tgtaggccgt atgatcgtcc ctactgtaaa atgaatcatt
     2281 tgcatttccc ctatattggc tttgtctaca tctattatac aaaatgaaaa taaataaatc
     2341 catacatttc gtgaaaacat gtgggctata ttttagcccg caagggcctg ggagtgcggc
     2401 tggccagaaa aaatcatctc ctggaaaagg aaacttgacg ctccgcgtcg aaatagtata
     2461 tcataggaat gttatgtatc cattttttag atttacctta aaagagtgtt ccctaaagaa
     2521 ggaatttcac aggtggatag agaataatat atacgtcagc gttattgaaa tgaaacaggt
     2581 gatatgagtg aaacggtttt ccagcgaatt tatcgaacag gtccggcagg ccaatgacat
     2641 cgtcagcgtc atctccgatt atgtgacgct gaaacggcgg ggccgcaact tctgggcctg
     2701 ctgccctttc cataatgaaa agaccgcctc cttcagcgtc gctgccgaca aaggattctt
     2761 ttattgcttt ggctgccatg ctcacggcga tgtcttccaa ttcattatga agaaagaaaa
     2821 cctttccttt tcggatgccg tcgaacgcct ggctgaacgg gcccatatcg ccctgcccgt
     2881 cgtcgaacgg tcggccgaag aaatcgcccg cgataaacag cgggaccgct tgtatcagat
     2941 caacgaaatg gcgggcaatt ttttccataa ctgcctgacc cagactcatt acgggcagcc
     3001 cggactggcc tatttccaca agcgccacgt atcggatgat accatcgagg cctttaagct
     3061 gggctttgct ccggaaagct gggataaact gacgacggct ttcatgaaaa aaggtatcac
     3121 tggcagggaa ttagtcctcc tgggactggc taaagaaaag aacggccact tttacgatgc
     3181 cttccgcgga cgggtcatgt tccccatccg cgacggccgc agccgcatcg tcggtttcgg
     3241 tggccgcgtc ctcgacgaca gcaagccgaa gtatctcaat tcgccggaaa cgccgatttt
     3301 caataaacgg cggctcctct tcgccatgga cctggcccat aaagcgattt atgagcaggg
     3361 gaaagccatc ctggtcgaag gctatatgga tgtcgtttcc gctcataacc ggggcatcca
     3421 taatgtcgtc gcgtccctgg ggacctcctt tacgccggaa caggcgcggc tcttgcagcg
     3481 ccaggccaag gaactcgtcc tgggctacga catggacggc gccggccgtc aggcgacgca
     3541 gcgggctatg gaaatcgtcc gcggcctggg catgcgcatc cgcgtcttgt ccttgccgca
     3601 gggcaaggac cctgatgaat acatcaatgc cgccgggccg gacaagttcc gccaggccgt
     3661 cgacgaagcg ccgaatgtgc tcgattacat gctcatgcgg gccttgcagc gcttcgacgt
     3721 gacgacgctg gaagggaaat catccattac gaccatggtc ctgcccgtcg tcgctgccgt
     3781 cgacaaccgg gtcctgctgg aagcgttcct gaagaaactg gcaggtcagc tgcagatagg
     3841 ggaaaacgcc gtgcgcagcg aattcaacaa gtatgtcgct gcccatccgg aagcaggcca
     3901 gcagccggtc gtcatctctt cgtccgtatc ccaggaaaat gccgcagccc gcgggagcgg
     3961 tgccatggct gtagccgaag agaatatcct gcgtttcctc ctggaaaagc cggctgcctg
     4021 cgggcggatc cacgccaaag tatcggcgga cctctttgcc gatgaacggc ggcgccacat
     4081 ttaccagctc attttggata cgtatgcaca ccaagggatg tacacaccac atgacatcca
     4141 gcaaaaatta actcctgagg aggccgaaga agtagcacga attatggttc tacaagatgt
     4201 cccactggac gaaaatgtac tgatggatta tgtcaagcgc tttcgcatgg ccgatttaca
     4261 aaagcagtac ctggcgcaca gccgcctggc tgccacttat agccggactg gcgatgcaag
     4321 gctggccgaa gagcttgcag catgtaagaa aattaacgat gaaatgaaac aatggtcttg
     4381 aggagcagaa aggagcctgt acaccatggt gaataaaacg acagatgata caaaagtaac
     4441 gaagaaaacg acgaaaaaga cgacgcgccg caagccgtcc aagaagaagg acgctgccgc
     4501 caagaaaccg ttgagtgaaa aagaacgcca ggccctggcc ctgcagcaca tcaagcagct
     4561 cctgcaaatg gggcagaaga agggcagcct gacgtatacg gaaatcatga acctcatgga
     4621 agaagacgaa ctgacgccgg accagatcga caagatgtat gaagtctttg ccgacaaggg
     4681 catcgacatc atcggcgaag acgactccat ggcggaacat gatgatatag atgatgaaga
     4741 tacggaaaaa gtcccggacc ttcacaatgt cgacctcagc gtgccggaag gcatcaacat
     4801 cgatgacccg gtccgcatgt atttgaaaga aatcggccgc gtccccctct tgtcggcgac
     4861 ggaagaaatt accctggcca aggccatcga aaagggcaat gctcccgatg caacagaaga
     4921 agacatcaaa gccggcacga ttgccaagaa gaaactgacc gatgccaacc tgcgcctcgt
     4981 cgtcagcatc gccaagcgct atgtcggccg gggcatgttg tttctggacc tcatccagga
     5041 agggaacctg ggtctgttga aagccgtcga taaattcgac tacagcaaag gctacaagtt
     5101 cagtacctat gcgacatggt ggatccgtca ggccattacc cgtgccattg ccgaccaggc
     5161 ccggaccatc cgcatcccgg tccacatggt cgaaactatc aacaagctga tccgcatttc
     5221 ccgccagctc ctgcaggaca aaggccgcga accgctgccg gaagaaatcg ctgaaaaaat
     5281 ggacatccct gtcgaacgcg tccgggaaat catgaagatc gcccaggaac cggtatcgct
     5341 ggaaacaccg attggcgaag aagaagactc gcacctcggc gatttcatcg aagactcgga
     5401 agccgtcgcc cctgatgacg ccgcctcctt catcttgctg aaagaacaaa tcgaagacgt
     5461 cttttcctgt ctcaccgacc gggaacgcaa agtcctctac ctgcgcttcg gcctcaaaga
     5521 tggccgtccg cgcacactgg aagaagtagg ccagcacttc aacgtcaccc gtgaacgtat
     5581 ccgccagatc gaaggcaagg ccctgagcaa actgcgcaac tggggcaaac gggaaaaaat
     5641 caaggatttc ctctaagccg aaatctctcc ttgacgccat atgctaattc gcgtataata
     5701 atttggtagg gccttgcgcc ttatcttttg ggcctatagc tcagtggtag agccgccggc
     5761 t
//
LOCUS       DAWABZ010000113         5673 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_3693_length_5673_cov_2.865255, whole genome shotgun sequence.
ACCESSION   DAWABZ010000113 DAWABZ010000000
VERSION     DAWABZ010000113.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3693_length_5673_cov_2.865255"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1072..1081
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1470 a         1350 c         1463 g         1380 t
ORIGIN      
        1 aatacaaaca cctcaaaggc aacttcggca cggcttggca gaaccagcag aaagaattcg
       61 acggcgttcc cggtacattc ctgttcacga cgaactgcct catgccgcct cgtaaatcct
      121 acatcgaccg cgtcttcacg acggaagtcg tttcctttga cggcgtaact catatcggcg
      181 aagacaaaga cttcacgccg gtcatcgaaa aagccctgga actcggcggc tatgcagaag
      241 accagcattt caccggcatc aacggcggca gcaccgttac gactggcttt ggtcatggca
      301 cggtcctctc cgttgctgac aaagtcatcg acgctgtcaa agccggcgct atcaaacact
      361 tcttcctcgt cggcggttgc gacggcgctc gtccgggccg taactactac acggaattcg
      421 ttaaacagac cccggctgat acggtcgtcc tcaccctggc ttgcggcaaa taccgcttca
      481 acgacctcga cctcggcgct atcggcggct tgccgcgcat catggatatg ggccagtgca
      541 acgacgcata cagcgctatc caggtagctg tcgctttggc aaaagccttc gactgcggcg
      601 tcaacgatct gcccttgacc ctcgtcctct cgtggtacga acagaaagct gtctgcatcc
      661 tcttgaccct cctctccttg ggcatcaaga atatttacct cggcccgaca ctgccggcct
      721 tcgtctccaa gaatgtcctc aactacttgg tagaaaactt caacatcact ccgaccagca
      781 cgccggaagc tgacctcaaa aagattttgg gataagaggc cggagtttat agcttgtagt
      841 ttgcagagaa aacctttaaa tttaaagcca tccgctaaca actaacttgc tagccactaa
      901 caactttaaa gggcttgtcg tcgtgcgaca agcccttttt ccttgacgcc ttttttttct
      961 cttgttataa tgaattaaca caaaagaaca aatacaaaca ttatataatg agaaaagtat
     1021 gtaaggataa acatgaggtg aagtgtatgt taccagcaga acgacggcag tnnnnnnnnn
     1081 ngtacaagaa caaagctgta acggtcaatg acctggccca gcagcttgat gtcacgacga
     1141 tgacaatccg gcgggatttg cagcatttgg aagaaggcgg cttagtcgaa aaaagccacg
     1201 gcggcgctgt tttggtagaa tcttctgtca aagaagcgac gtatcgcaat cgtaaattag
     1261 tccacattga agaaaagcgg cgcatggcgg cagcggcctt gcctcttatc gaatcgtcta
     1321 tgagcatcta tctcgatgcg ggtacgacga attttgagct ggctgaattg atggccaaac
     1381 agcgctggga taatctgacc gtcgtgacca atgatttagc cattgcccag gtcctgactc
     1441 cggtcatggg gctgtatgtc atcctcttag gcggccatat tgacgtcgaa tccagttcga
     1501 cgtgcggcgt cttggcgacc aatatggtcc gggccatgca tttcgatatc tgctttttag
     1561 ggactcaggc catcacgccg gattggcgca tcatgacggc caatgctgag aaaatcgatg
     1621 tcaaacgggc ttgtcttcag gcatcggata aggtcgtcct cctggcagac cattctaagt
     1681 ttaagaaaca taaactctat tatatcctgg acctctggga tatggatgtc ctcgtcagtg
     1741 actatcaggc cagtgcagaa gaagcgtcct tatttgaaga acaccaggtt cagtttatcc
     1801 gcgtttaagg gggagagcac atgcatcatt ttgtcatcat tgctgatgat tttacgggag
     1861 ccaatgatac aggcgtccag ctttgcaagc acgggattcc tgtcgatgtt atcctggata
     1921 cggcacagat agaagccaat ggcaacagcc tgtccatcga cagcgagagc cgcgtcgttt
     1981 ccggccagga agcctatgac cgcgtgtatg cggccgtaca gcaggtacag caaaccggtg
     2041 gctgccgttt tctgtataag aaagtcgatt cgacgctgcg cggtcatttg caagaagaaa
     2101 tccgggctgt tgtcgatagt tatgcgccgg aactcatcat ctttgcgccg gcttatcctg
     2161 agcaggggcg gactgtcgaa gcggggcggc tttgcgtcca tgggacaccg ttgctggata
     2221 cggaaatcgc ccgtgatccg cggaatccct tgcaggaaga tcacgtacaa cggattttgt
     2281 cgacgtgcct gcagcagctg gtctgtcact ataccctcga tgccatcgag gacgatcgtc
     2341 ttgactttac cggattagct tattcctttg atacgatgga gcaggcgcag ctggaacgga
     2401 ttgccgaaaa agccggtcag acggggaaga aagtcctgtg gatcggttct gccggtttgg
     2461 cccaggggct attacagata cagctgcggg agctcccggc catggccgtt attggcagca
     2521 tcagcagcaa gaccatggac cagctggcgt attgccggaa ccggggcctt cccgtcgtgg
     2581 cactggacat gagggccctt tatgaaaatc aggatggaag gacagcgatt cagaaaatcg
     2641 taaacttttt gcagtctggc cagagcgtgg tcctcacggg ggctgcctgc tgccaggatt
     2701 atgaagactt tgctgcctat ggccgggaaa aggggatttc tacggatgaa ttggccgaat
     2761 ttaccaaaca gaccctaagc cggatggtgc cggctatttt gcaggaaacc gccgttagcg
     2821 gtttgttttt gaccggtggc gatacggcca ttgccgtcat tttgcagtta ggggccagcg
     2881 ggtcccatat tgaacgggag atagtgcccg gttttgtgca aggccgtttg ctgggaggca
     2941 gccaggaagg attgcctatc gtcaccaaag ctggtgcctt tggtacggaa gaagatattt
     3001 tccgctgtat ccaggaactg aaatacaatt aattacattt tttttagttc taaaatgttg
     3061 ttttgtgtta taaaatcata catatagaga aatgaaaaaa gccaggaatg agcatgagta
     3121 tgccatctct ggcttttttt tctgtgattt tcttggaagt tgcctctgaa atatgatgaa
     3181 aaaatagcat ggtgtatata cgaaaaagcc attgacttat tagagaaact ctcctataat
     3241 ctaattaaca aaaacaaaca gataagaaca taatcaaaca aaaaatgatt gtgacaaagg
     3301 aggaggtaca gtggcagtaa attttgtagg cattaccatg ggcgatccgg ctggtatcgg
     3361 gccggagatt tcccttaaag ctatagatgc taaaacggag taccaacaat atgtcttgat
     3421 ttatggcagc tacgacgtgc ttcaatacta tcatcaacgg cttcagctga cgacgccgct
     3481 ggtacgcatt gaaaaggtac aggacttcca gccagggaaa atcaacgtca tttctgtcgt
     3541 cgacctggct atcggcaaag atattgaaat cgggcaggta tcacccgttg ccggtgatgc
     3601 ggcgtatcaa tatattgccc gggccatttc tgatgctttg aaaggcgata tccgcgttgt
     3661 cacgacggct cccttgaata aggaagccct ccataaaggc ggtcacaact tcgacggtca
     3721 tacggaaatc ttcgctaccc tgacgaagac gaaatcttat acaatgatgc tttggagcga
     3781 acacatgtct gtcgtccacg tatcgactca ctgttccctg cgggaagcct gtgacagagc
     3841 gacgaaagcc agggtgctgg aatgtgttca tctggccgat aaggcgatga agcagttggg
     3901 cgtcgaaacg ccgcggatcg ctgttgccgg cttgaatccc cacagcggcg aagccgggct
     3961 ctttggccgg gaagaaattg atgaaatcat cccgtccagg cggcccagca ggaaggcctc
     4021 gatgtcgatg ggccggtccc gccggatacg gtcttcttga aagcctataa tggcgtctac
     4081 gacatcgtcg ttgctatgta ccatgaccag ggacatatcc ccatgaaaat ggtagccttt
     4141 gattccggcg tcaatgtcac gctgggcctg cccatcatcc ggacatccgt cgatcatggc
     4201 acggccttcg atattgccgg caagggcatt gccaaggcgg acagcatgtg ctatgccgtc
     4261 gatttaggaa aaagatttgc agaacattaa attttacata tattcacaaa ggagcgagat
     4321 acattatgtt taaagcagaa ggtttgatta caccggttgt tacggcgttg acggaaacgg
     4381 aagaattcaa caaaaaagaa taccaggctt ttatcgacca cttaatcaaa gccggcgtcc
     4441 atgggatttt cccgctgggg acgaacggcg aattctatgc attttcggct aaagaaaaac
     4501 tggaaatcat cgaagctgcc gtcgaagccg tccagggtcg cgtccccgtc tatgccggca
     4561 cgggctgtgt tacgacgaaa gaaacaattg aattgtccaa agcagtccag cagatgggcg
     4621 gtgtcgattg cttatctatc atcaccccat attttgtaaa ggcttcacag gctgacttga
     4681 ttgaacatta cagtgccatt gccgaagccg tcgatatgcc gattttgttg tacaacatcc
     4741 cggcgcggac aggcaattcc attgactgga aaaccgtcaa gaccctggca aaatatgaaa
     4801 acatcatcgg catcaaagat tcgagcggca acttcgacaa catgctgaaa tacatcgaaa
     4861 atacagatcc ccgccttaac gtattggccg gcagtgactc cctcatcctc tgggccctca
     4921 tggccggcgg taccggtgcc atttcgggct gctccaatgt cttcccggaa ttgatggtca
     4981 gcatctataa ctactggaaa gaaggcaaca tcgaaaaggc caatgaatcg cagaagaaaa
     5041 tcagagcttt ccgcaatgtc atgcagatgg gcaatccgaa ttcggtcgtt aaattagctg
     5101 tcaacatgcg cggctatcac gtaggaccgg ctcgccagcc gtcgaactgc acggatccgg
     5161 ctattaaaaa agcgctggaa gatgttttca aactgtatga ataataaagg caaggagatg
     5221 taactcatgg ctaaggttgt aatttcacat cgtcttcatg atgatggtat ggctgttctt
     5281 gaaaatgctg gggtagatgt cgttattacg aatagtggcg atcctaaagt tatgttacca
     5341 gaattaaaag atgcggatgg ggttattatt cggattggct ctattgatcg tgaaacaatg
     5401 gaacaatgcc ctaaattaaa ggttattggt cgccctggcg ttggtgttga cgatgtcgat
     5461 gttgaagcgg ctattgaatt agggattcct gtcgttattg ctcctggtgc taatagtcgg
     5521 tctgttgcag aacatgccat ggcaatgatg tttgcttgtg ctaaagatat gatgcgcagt
     5581 gatagagaaa tgcgcaaagg aaattttggt atccgcagtt cctataaagc ttatgaatta
     5641 ggtggaaaaa cgctgggatt gcttgggtat ggt
//
LOCUS       DAWABZ010000114         5637 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_3722_length_5637_cov_2.776424, whole genome shotgun sequence.
ACCESSION   DAWABZ010000114 DAWABZ010000000
VERSION     DAWABZ010000114.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5637)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5637)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5637
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3722_length_5637_cov_2.776424"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5059..5068
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1329 a         1571 c         1373 g         1354 t
ORIGIN      
        1 ccatcacata gatttttgtc tatgtcatag attatacaag tgatatagat gattgtcaat
       61 atgataggtt caaaaagaag gactgccaca tgaaggcgtc tgccctcgtg cgacaatccc
      121 tttccaaaga gtatgtacta tctgctgaaa actacaaaca ctgtacgata aaccatcatt
      181 ttcccgtata cgtcttgcga gcagcgacga cttggcggat gtggtcggct acggtcgtac
      241 agcagccgcc gacggcgacg gcacctgctt tcatgtaatc cagggcataa tcgccgaaac
      301 ggcggtccgt cccgacgccg tgccaggtct tggtctgcgg gtcgtattct tcgccactgt
      361 tcgggtagac gatgatcgga aggtccgatg cccttttcaa ttcgccgatg aggctggcaa
      421 tgtattccgg cttcgtgcag ttgacaccga tggcctgcag gtgcggatag ttacgggcca
      481 gctggcgggc acaatcggcc aggagctggc cttcgcagtt gtggcggccg tcgcagcagg
      541 aaaagctgat ccaatagggg gcgcccagtt cttcggcgat ctgcgcttcg acttccgctt
      601 ccatcaggga cggttccgtt taaaagagca ggacgtcggc accggcctgc cacaggagtt
      661 ccgcccggcg gcgatggaag tcccgcagcg tatcggcact gacgccgtaa tggcccttat
      721 attcggaccc atcggccaaa taagccccgt acgggccggc actggccaga cagagcggcc
      781 aggaccggcc ggcctgcttc ccttcggcgt cccaccattc ctggcgggcg tccaaaaaga
      841 cctggactgc ccgggtgatg agggcttctg cctgttcgcg gctgtacccc gtttccatca
      901 ggcccggcaa agaggcctgg taactgcagg taatgcccgc atcggcaccg gcccgaaaat
      961 actggatgtg gacttccttg accagttccg gccggtcggc caggacgcgg gccgtccaca
     1021 gtttgttgtt caaatcggcc ccgagatttt ccaaagctgt ccccatggag ccgtcgagga
     1081 ccaggatgcc ctcgcggtcc aaaatctgtt tcaatgtact tttcattttc tttccccttt
     1141 cacaggcacc gtcccataag cgacgacagg cggattttcc cctcgttcct gcgccggcag
     1201 gaaacggaac cagaaatcat tgacggacga tcaattccag cgtctccggt acctgcggca
     1261 gatgctggac gaggatggcc aggccgccga ggatgtagag cagggccatg atgggcacga
     1321 cccgttccat gacggccgaa atgcgccgca agccgccgaa gatggtgacg aaacaggtga
     1381 ggcccagaaa agcggctgac cagagcgggt cgatgccgaa ggcttcccgc agggcgccta
     1441 cgacggattc cgtctggacg ctgcacgtcc agggacccag ggtaaaacag gcaatggcca
     1501 acaggaaagc ccctttccgc cagcccaggg cgttgcgcat gacaaaggac cggtcgcaca
     1561 tgtattcatc catggattca gaatacttga cgcggaagcg ctggcccaaa atgatttcgc
     1621 aggccttcgt cgacatgccg agacagcccg agacccacat ccagaagacg gcgccggggc
     1681 cgccggaaac gatggccgtc gccacgccgc cgatattgcc gacgcctatc gtattggcca
     1741 tggccgtaca agctgcggcg aaccccgata ccgacccctg gccgctgcca tggtgctgga
     1801 acatcttgcc aaaagtattg cggaaatgaa acccgatgtg ccggaaatta tagtaaaaat
     1861 gaaaacggat actcaaatac aagcccatac tgaccagcac gacaatcatc ggcgtccccc
     1921 acaaagcatc ggagaccgcc atcaatccat tcaaaatcat agttttcccc caatcaatac
     1981 atagtctgac ttactaagaa tgacataagc catggaaaaa gtcaatgatt ccttccattt
     2041 gtcacgtact tttcgatatg atacaattta tataatacaa ttaatttgtt catatctttc
     2101 atttttattt tggaaaggtg gtttttcttt gaaaattcca aaaaacaact tgcaggcaag
     2161 tattgtcctg cccagtatct tattcattgg gatcgtcatc ataagctgta tcttctttcc
     2221 cgaccggaca gatacaattt taagtattgt aaaagaatat atattcagtt cattcagctg
     2281 ggtatacatt ctttgtgtcg ccttcttttt cctcttcctg gtcatcttat gcctgggaaa
     2341 actcggtgac atccgcttgg gaaacgaaga tgaaaaacca gaattttcct ttttttcctg
     2401 gttgtgcatg ctgttttcag ccggtatggg cattggcctg atgttctttg gtgtagccga
     2461 accgatttcc cacttatcgg cccccttgca cgggtcggct tcatcactgg tccaaacgaa
     2521 ggaagccatg ctgaacaccc tgttccattg gggaatccat gcctgggcga tttacggtat
     2581 catcggcctg gccctggcct atttcggatt ccgctatcag cttcccgtaa ccctgcgttc
     2641 cggtttttat cccctcctga agcatagact gaacggtttc tggggcaacc tgatcgatat
     2701 cgtcgccctc tgtgcgacga ttttcggtct gacgacgacg ctgggcttcg gagccatgca
     2761 gctcaacgcc ggcctgagtg ctgtcggcat tttttcgaca gcctcttttt cccacatcgt
     2821 cctcttgatt gtcatcaccg tcggcctggc catcttgtcg gccatttccg gcgtaagtaa
     2881 ggggatccgt tacatgagcc agggaaatct gatcctggcc atcgccctgc tcctgttcat
     2941 cctcttcgcc ggcccgacgg tatatctctt gtcggccttc acagaaaacg taggctacta
     3001 tctgacacac atcatcgaac tgtctttccg cactttcgcc tacgaaagca ccaaagaaga
     3061 atggttcacg aactggacca tcacctattg ggcctggtgg atttcctggg cgcccttcgt
     3121 cggtctcttc atcgctaaga tttccaaagg ccgcacgata cgcgaatttg tcgtctgcgt
     3181 cctcctggtc cctaccctgt tcaatatctt ctggatgacc gtgttcggca acggggccat
     3241 ctgggtcgac ggccagaccg gtggcatcct gacccaggcc gccgcggaaa cggaaaagct
     3301 cttattcctg ttcctggacc aattccccct ggccctggtc acctcgctga ttgccctgct
     3361 ggtcatcgtc attttcttta tcacttcggc tgactcgggg attttcgtca tcaacagcat
     3421 cgcctcgcaa gggaagaata cgtttcccaa gtggcagact gtcttatggg gaaccctgct
     3481 tgccctgctg tccatcgccc tgctctattc cggcggcctc caagccctgc agacgatgac
     3541 cgtcatcatc gccctgccct ttgccgtcat catgatcctg atggtgttct gcctgctccg
     3601 gggcttgacg atcgataacg cctatttcag cagaaaattg tcagaaagta ccgtctactg
     3661 ggatggctcc cattggcagg accggctgaa aaaaatcgta tccgtcggga ccttggacga
     3721 tttccagcag ttcctcaaac agaccgtcga cccggctttc caggaactgc gccaggaatt
     3781 tctgcggaat ggaatttccg tccacatcca gggcagtgat gatagtcaga cggcctgccg
     3841 ggaattgatt gtcgaaagcg ggagcctccg caatttcatt tatggcatcc gctaccgggc
     3901 caatgacgta tccgatactg tcgtcggctc cctgtccttc ccgaccatta cggaagagga
     3961 aaattatgaa cccatttgtt attttgccga cggccgacgc ggatacagca tcaaatatat
     4021 gcagaaagat gaattgatca ccgacgtcct ccgtcaatat gaacgatatg tcaagatgct
     4081 ggcagccggc aaacacgact tatacctgac agataaagta catcccggac aaggaggaaa
     4141 atcatgaacc gctggaataa cttaatcgat ttgaaaggcg actcagcccc gatggaagtc
     4201 atcaatcagc gcatcttatc cgacgccacc atcgaaggca cgaacttgat cgtcttagtc
     4261 atggccatca tgatcgcctg catcggcctg aacttgaaca gcgtcgccgt catcatcggc
     4321 gccatgctca tttccccgct gatgggcggt atcgtagcga cgggctacgg aatggccaca
     4381 tatgacgtcc cttttatccg caaatccctc atcaaattat ccttccaggt cggcctggcc
     4441 gtgctgacag cttcgattta ttttctcctt tcgccgttta cctcgccgac agatgaaatc
     4501 ctggcccgga ctaccccgac aatctgggat gtcctcgtcg ccttatgcgg cgggattgcc
     4561 ggtgcggtcg ggaacacgcg gaaagaaaag agcaatgtca tccccggcgt cgccatcgcc
     4621 acggcactga tgccgcccct ctgtacggcc ggctatgggc tggcctccgg gaaccacacg
     4681 tttttcctgg gtgccttgta cctgttcttc atcaatgcct ttttcattgc cttgtcttcg
     4741 ttcttggtat ttaagattct gaaagtcccg cctcacggca atgtcgctga agctcatttt
     4801 cgcaaacagc gtctcatttt agttatcctc ggcctattaa ttacgattcc aagcatctat
     4861 atggctcatc taacggtaaa agatgcgctc caggaagtcc aggtcaaatc ttttatagaa
     4921 acaaacatga attttgaaaa aactagtgtc gtttcttaca cactaaaaga taagaccttg
     4981 acaattgatc ttgtcggggc tgtcctgacg gaagaacaaa tagaccagct taaatcgaac
     5041 ataaataact atccacgtnn nnnnnnnngg gaaacaggaa tctgaacact gaggaagtgc
     5101 agcgattaat caattcacgt atagaaaaac aaggcaaagc cgaaaaagaa gcttcgtaca
     5161 agatgctgac cagtaaatat tacccagctt acgaacgtgc tgcagcagac cagcaggtca
     5221 tccagattct aaacagccag gctcctttat tattcccgga aatccagtct gtccggggtg
     5281 gcacgctaac agccttgcat cgagataacg atacgaaaga cggtacattt ggctacgacc
     5341 gtttcatggc cgaagttacc gtcagaactc cccttagtgc cgatgacgcg ctgcgtctgc
     5401 atgattggat caaaggtcag gtaaacatgc ctgtcattct gaatgtccag accagcaatg
     5461 ctcccagcga atactatggc aacgggattc aatggtaaaa tcaaaactgc tttaagcagc
     5521 tgaaaagtat accgaccccc aattgttaga ccaaaactct aacgattggg aggtcggttt
     5581 tttatgtcta aatatagtta cgaattcaag aaaaaaatag ttcttgaata tttagat
//
LOCUS       DAWABZ010000115         5539 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_3805_length_5539_cov_2.742159, whole genome shotgun sequence.
ACCESSION   DAWABZ010000115 DAWABZ010000000
VERSION     DAWABZ010000115.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5539)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5539)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5539
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3805_length_5539_cov_2.742159"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2573..2582
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1277 a         1621 c         1477 g         1154 t
ORIGIN      
        1 aaagaggctg tcttaatgcg acagcctctt tttaagagga acaacaccag gaaggactgc
       61 ttgtatgatc attccgacgt tgaaatggga cctcatcatg agagccgttt ctatttcgtc
      121 ttacttacca ggccgtatcc gcctgcacag taaaaaactc attggcaatg ccgaattggg
      181 ccgcaagggc tactcctaca ttgccagtta taaagaaatc gacaaggtcg atgtcaatgt
      241 ccttacgggt tccgtcctga tcacctaccg gccgcaggtc ctgcgggcca atcgggaact
      301 cgtccgggta gagaactata tcatgaccca tgttgaaagg agaaggtaat tatgtcttat
      361 gcctcaggtt ttatggtggg cacgtcgatc ggcaaagccg tatacgactt gttccacaat
      421 aagaagggca aagcggctgc cgcagctgcg cccgccggtg ccttccggcc tcaggtccag
      481 gcggcaaagg gagtaccgga agtgccccgt ttcgcctgcg tatccgtctt gaaaggccgt
      541 cgccgctatc gggcagcggc cctcgtcggc aatgcggaac tggcccgctt gatcgaagaa
      601 aaagtcagca ccctgcccaa tatccagttc atccaggtca gcgtcgtcac gggcagcatc
      661 ctggtcttcg cccacagcga agggaccttg gaccggctgg aaaacttctt ccgcttccgc
      721 ctcttcccga atgtcgtcga aggcatcgtc agtgccgtct gcgatgcaga aaaacaggaa
      781 ggccagaaaa aggaagagac gacgtatctc aaagcggtcc gcgatacggg ggcttttttc
      841 agcagtatca tccgcaatga atccagtcgt ttttttgact tgcgcacagt cgctgccttg
      901 ctcctgaccc ttcgcggctt gcgcaaagtc gtattcatgg gacagcgccc gtcaggacca
      961 cagatgctgt ggtgggccct gtcgttgttg agggggcgtt aagtcatgta catgcgcaat
     1021 accatcttcc tgcacagcga gatccccagc cgcatgcgcc ctgtcctgag cgtcaagctc
     1081 cgccaggtcc gcggcatcgt ctcggctgtc gtcgaagaaa agaaggtcac cgtctactat
     1141 gtgggacagc tggacctgcg ggcactgaac ctgttcatcc gtgcctttga agaaaaatat
     1201 ggccgcaaga aggaacagaa agacgacgac atggatgttg ctacgtaccg ccgggaagcc
     1261 atcatctccg tcggcggctt catcgccatg aacatcctgc gcaaggtcaa cccggaattc
     1321 tacgggtcga tcctcttgtt tcgccgatta tttaccttat atattgcacg ccgctacatt
     1381 aagaacggca tcatggggct cgtaaaggac catcaggcca atgccgatac cctgacggcg
     1441 acggcagtcg ctgcgtccgt cctggctggc aagccagaat cgagcctgac cctcctggcc
     1501 ctgtcgaatg gggccgaaat gatgaccgaa tacgctgccg aaaaggcacg ccgtcagatt
     1561 tcgggcctcc tcaaactgga ccagcgcgac gtctggctcg tcgaaaacgg ccatgaacgc
     1621 aaggtccctg tcgaatcgct gaagcggcgc gacctcatcg ccgtccactt gggcgaaaag
     1681 atttgcgtcg acggcagcgt cgtcagcggc aatgccgccg tcaaccaggc ctccatcacg
     1741 ggcgaatcca atccggctat taagcaggaa aaattgccgg tctatgcggg cagtgtcatc
     1801 gaagcgggcg acttggtcat ccgcgtcgaa aaggtcggcg ccgacacgtc cctggcccag
     1861 atcatccacc tcgtcgaaga agcccagacc cgccgggcgc cggtccagaa ctttgccgat
     1921 aagatggcca accacctggt tcccatctcc ttcatcggcg cagccatcgt ctacggggcc
     1981 accaaagact ggcagcgcgt cctcaacctc ttgttcatcg acttctcgtg cggcctgaaa
     2041 ttgtcgacag cgacggccat ctcggcagct atcggcgtgg cagcccgcaa ggggatcctc
     2101 atcaagggcg gcaactacat tgaaaacctg gccgacatcg acacggtcgt cctcgacaag
     2161 accgggacca tcaccatggg cgtcccccag atcgaccaca tcgaaaccgt cgaaggcgta
     2221 gacgataagg aaatgatcct cctcgccgct tcggctgaaa tgcactccgt ccatcctctg
     2281 gccgtagccg ttcagaaata cgtcaacgcc cagggctggc agacgccgcc tcatgcgtcg
     2341 tcggaaaccg tcgtcgcccg cggcatgaaa gcgaccgttc ccgattttga aggctacgaa
     2401 ggcggggaca tcctcgtcgg cagccgccgt ttcatgaccg aagaaggcgt ggaaggcatg
     2461 ccggcgacgg ataacaagac ctggggcaag aacctcttgt acatcgcccg caacggcgaa
     2521 tatctcggct tcttggtcat ccaggacccg gtccgtccgg gtatgaagaa gannnnnnnn
     2581 nngaccctga accgcatgcg ccgcctgggc atcgacgaag tcgtcatgct caccggcgac
     2641 tcgaaggacg tcgccgccga agtcgcccgc gacatggata tcgactcgta tcatgccgaa
     2701 atcctgccgg aagacaaggc caactacgtt atgaagatgc agaagcgggg caacgtcatg
     2761 atggtcggcg acggcatcaa cgacgcaccg gccctggcct ttgccgacat cggcgtcagc
     2821 ctcggcggca acaagacgga catcgccgct gaatcggcag ccatcaccat ccgttcggaa
     2881 gacccgtcga aactgtacga tgccctgtac atcggcaggg aaacgatgcg ggccatcaac
     2941 cagaacttca cggctaccat tgtagtcaac tcggcggcca tgctgctcgg cgccttaggg
     3001 aaaatcagcc ccctctgggc tgctgtcatc cacaacacag ccaccctggc cgtcgtcctc
     3061 aacagcgtcc gcatcctgaa accggcccgc ccgaccagac gcccggcata gggaatcgcg
     3121 catgccaatg gtttgtaggg acccgcgcat gccaatggtt tgtagggacc tgcacacgcc
     3181 aatggtttgt aggggctcgc acgtggcgag cccgtccggg agatgaatac attcccatat
     3241 caagcagaaa cgggcgcccc acgtggcgcc cctacaaaag gacttgtcgt tatgcgacaa
     3301 gtccttttta taaatgaatt tcaatagcaa ctattgacag ggtttgtgaa aaaatatata
     3361 atgtttatgg taatgaaatg tattatcatt aattagaagg gatgtgtgag tattatttac
     3421 ggagctgaaa ggagcgatgt aaatgagtgt cgtcatcgta ggtggaaatg attgtatggt
     3481 ttgtcgctat aagcgtattt gcaaagagta cgattataag gcaaaagtct ttacacaggt
     3541 accaagcgat ttcaaatcga aaatcgggtc gcctgatctt gtagtgttgt ttaccagtac
     3601 cgtgagccac agtatggtcc gttgcgccat caaagaagcg cagcgcaaga acgtcgaagt
     3661 cgtccgttcc catacgagca gctcgaacgc gctgaagaac atattagccg cccggatgga
     3721 agaggccgtc tgaggcaagc aaaggggagt tagtcatgaa aattgtttca ttagccgata
     3781 tgaaagtcgg ccagtcgggc gttgtcgatg ccttgaaggg acggggaaat atccagcacc
     3841 gtctcgtcga catgggcgtc gtcaagggct cgcatatttc agtcttcaaa aaggccccgc
     3901 tcggagatcc cattgaggta aaagtcaaag ggtgcgcact ggcactgcgc atgaatgaag
     3961 cggcgatgat ttccgtcgcc gtagaacaat aaagggggta catagtagtc atggaaaatg
     4021 cagcgaagat caagattgcc ctggccggca atccgaactg cggtaaaacg acgattttta
     4081 acaacattac cggcgctaaa cagcacgtcg gcaactatcc tggcgttact gtcgaaaaga
     4141 aagaaggcca tcgtaccttc aacggcaagg acctgctctt cgtcgacctg cccggcacat
     4201 acagcctgac ggcacggtcc ctcgatgaag tcgtagcccg taacgtcatc atcaatgaaa
     4261 aaccggacat catcgtcaac gtcctcgatg cgtccaacct ggaacgtaac ttatacctgg
     4321 ccgcacagct cgtcgaactg ggccgcccca tggtcatcgc cctgtacatg atggacattg
     4381 ccgaacgcat gggcatccag atcgacctca agaagctggg caaacagctc ggcgccgtcg
     4441 tcgtgcccct cgtcggcagc aagaacatcg gcacgaaaga actgctcgac gccatttcca
     4501 gtacgcagac ccagaacctg gtcaatgcca aagtcgatta cggcgctgac gtcgaatcgg
     4561 cgattgccaa cctgaccgat gccatcgaaa aaatgggcat catcaaatac ccagtccgct
     4621 ggctggccgt caagctcctg gaaaacgact ccgacgccat cgccaaagtc cgcgctatgg
     4681 aagggaccca gtccatcctg gccctggctt cgactctgcg cgattccctg gccaacaaga
     4741 tcgatttgga cttctatttc gctcaatgcc gatatcaatt cgcaactgat gtatttaata
     4801 aatcgattat caatgtcggt tcgagcgact ccttgtccga caagatcgac agcgtcctga
     4861 cccaccgtta cttagggatc ccgattttcc tggccctcat gtggctcatg ttcgtcgtcg
     4921 tcatcaacgt tggcgcctac ccgcagggct ggctcgacac gggcttctcg atgctcggtg
     4981 actggtgcag cgatgtcatc gaagatgaac agctccgctc cctcgtcgtc gacggcgtca
     5041 tcggcggtgt cggttccgtc ctttctttcg ttccccttat cgtcctcttg tacttattca
     5101 tcagccttct cgaagatacg ggttatatgg cccgtgccgc cttcctcatc gaccgcgcta
     5161 tgcgtgccct ggggctgcac ggcaagtcct tcattcctat gatcctcggc tttggctgca
     5221 acgtaccggg catcatggcc gctcgtacac tggacaatga aaaagaccgt ctggtcacga
     5281 ttctggcctg cccgttcatg agctgcggtg cccgcctgcc ggtatacacc ttgctcatcg
     5341 ccgctttctt cggcgcttcc ggccacggtg gtacggtcct cttcggcgta taccttttag
     5401 gtatcatcat ttccgtctgc gtcgccctgg tcctccgtca tacgaccttt aagggcgaac
     5461 aggaaccgtt cgtcatggaa atgccgccgt atcacattcc gaccttgaaa ggcgtcctca
     5521 tgcacatgtg ggaacgcac
//
LOCUS       DAWABZ010000116         5511 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_3824_length_5511_cov_3.080462, whole genome shotgun sequence.
ACCESSION   DAWABZ010000116 DAWABZ010000000
VERSION     DAWABZ010000116.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5511)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3824_length_5511_cov_3.080462"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1013 a         1464 c         1632 g         1402 t
ORIGIN      
        1 accacgctct ttgtgagcat tttttatttt caaaagtgtc aaactcggga ttataccact
       61 ttcgcttaat gaagccaact agaaacatcg ttcgctataa aaggagcaaa aaaggggctg
      121 tcctttgaag acagcccctt tttagggggt tagcagttag gggttcgtgg ttcgtggcta
      181 gcggctaact gctacctgct gacggccagc acccgttcaa agaggttctt ggccaggggg
      241 ccgaagccgt cgcgttttag cgacgagtag gccagggccg gccggtctga cgggaagagt
      301 ttgttgagct gggccaggtt gcggttcttt tcattggcct tgagcttgtc cgccttggtc
      361 cctacgatct gcaggggaac gccggcgctg gccagccagt cataggcctg ctggtcgatg
      421 gccaggccgg gatggcgcag gtcgacgagc aggcagagga cgcagaggtt cggcgactgc
      481 gtgacgtatt cgccgatgaa ggcactccac atgtcacggt tcctgccgcc agtcttggca
      541 aagccatagc ccggcaggtc gactaagtag aacggcaggc gctgctcccc ttcttcttca
      601 tcgcgccgcc gcagggctgc gctgaagtag ttgatagtct gcgtcttccc cggtgcgccg
      661 ctgacacggg ccaggccgcg gtggttgcac agggaattaa tcaggctcga cttgccgacg
      721 ttggaacggc cgaggaaggc gacttccggc acgttccctt cggggtactg gtcgcggcgg
      781 accgccgtcg tggcataggc cgccttgatg atatcatacg aagccatcag gacttcacca
      841 gtgcctgagc cagcacttca tccatatggt gaacgaagac gaatttgacg tcttttttga
      901 ccgactgcgg cacttcgtcc atgtcgcgct tgttttcttc aggcaagagg atcttcttga
      961 tgcctgcccg gtgagcggcg atgacttttt ccttgatgcc ccctacgggc aggaccgtcc
     1021 cgcgcaacgt gatttcaccg gtcatggcta cgtcgtggcg gacggctttg cccgtcaggg
     1081 ccgacgtaat ggccgtggcc atggtgatgc ctgccgacgg accgtcttta gggatggctc
     1141 cttcgggcag gtggatgtgc aagtccatgt tggcgtagaa atcggcatca atgcccagga
     1201 catcggcccg gctgcggacg taggtcacgc cggcttcagc cgattccttc atgacgtcgc
     1261 cgagctggcc cgtgagcagc aggcgccctt tgcccttgac ggcaacggct tccgtttcca
     1321 ggacttcacc gccgacctgg gtccaggcca taccggtgac gatgccgact tcgtcgtctt
     1381 tgtggaccgt ttccggcagg aatttgaccg gaccgagcag tttttccagg ttcttgacgg
     1441 agacgctgag cggtacttcg tcgttcatga gcagggattt gccgatacgg cggcagaccg
     1501 taccgatggt cttttccagg ctgcggacgc ccgattcgcg cgtatagcct tcgatgatat
     1561 ggcgcagaac ggcgtcggag aacttggcct gactcggttt aaggccattt tctttgatct
     1621 gtttcggcac cagatagcgc ttggcgatct ggaccttctc ttcttctgta taactcgtga
     1681 agtcgatgat ttccatgcgg tccatgaggg gccggggaat atcgccgagg acgttggccg
     1741 tcgtgatcca gaaggctttc gacaagtcga agggcagttc caggaaatgg tcactgaaag
     1801 tattgttctg ttccgggtcg aggacttcca gcagtgccga cgacgggtcg ccgcgcatgt
     1861 cggacgtcat cttgtctatt tcgtcgagga ggaagaccgg gttcttgacg ccggcctgtt
     1921 tcataccggc caggatacgg cccggcaggg caccgatata agtccggcga tggccgcgga
     1981 tttcagcttc atcgcggacg ccgcccaggg atatgcggac gtacttgcgg ttcatggccc
     2041 gggcgatgga tttggcgata ctcgtcttgc ccgtgcccgg aggaccgaca aaacagagaa
     2101 tcggcccttt caggctgtgc gtcagctgtt tgaccgctaa atattcgatg attcggtctt
     2161 tgaccttctt caggccgtaa tggtcttcat cgaggacttt ctgcgcttcg ttgaggtcca
     2221 ggcggtcctt ggtttccttc ttccagggaa gggccatggc ccagtcgagg taggtgcgga
     2281 tgatggccga ttccgccatc atgggcggca tggagtcgag gcggtccact tccttgagca
     2341 gggccttttc gatgtcttcg tcgagcttca tcttcttgat ctggtcgcgg agttcctgga
     2401 cttccgtgtc cttgtcgact ttgtcgccca attcttcatg gatggccttg atcttttccc
     2461 gcaggtagta ttctttctgc tgctggtcca tcttgccgcg gacttccatg ttcagcttcg
     2521 attccaggtt ggcaatgtcg acttccgtat ccaagagcga cgcaatgcgg cgcaggcggg
     2581 cttcgacgtc agccgtttcc aggagctgct ggcggatgac caggcgcaag ggcagctggg
     2641 atgcagcgaa gtcagccagg acgcccggtt cgtcgatgtt cttggcattt tcgctggctt
     2701 cttcgccgtc gcggagattg cccatccagt ccaggagccg cttgaggacg acccggcgca
     2761 gggcttcttc ttcgacttcg ttgcggctga tttcagccac tttgacggca gccccttcga
     2821 ggtaatcggc tttctgggaa aagccctgca tgcggacgcg ggaaatgccg ttgaccagga
     2881 tgcggaccag gccgccaggc atgcgcagga gctgttcaat cttgaccagg cagccgatgg
     2941 tatagacatc gtcaaaaccc ggattgtctt cgttttcatc gacctgggtc gctaccagga
     3001 gctgccggtc gtgttccatg gcgtagtcga cggcagccag ggacgtatcg cggccgatgt
     3061 cgatgtggct gaccagcttc ggatagacga cgatgccccg gagcgggaca aaaggcaggg
     3121 ccaggatatc ttgctcgttg agcacaacgt tttcgttcat acacttcacc ttcttctatg
     3181 gaaaaataaa actcatttcc ggtttaagaa atgagtttta ttgtacatta tatcattttg
     3241 cctctttcaa tacgaggtcg ggctcagcgc cttctttgac gcatttttcc gtgacgatgc
     3301 acttggctac gtcgtcgcgg ctcggtacat caaacatgac atcgagcatg atccgttcga
     3361 tgatggagcg aagcccgcgg gcaccggtat tgcgccgcag cgcttccttg gcaatggcct
     3421 ggagagcttc cggcgtaaat tcgagatcta cgtcgtccat ggacagcatc ttctgatact
     3481 gtttgaccag ggcgttcttc ggttctgtca ggatgtgtac catcgcatct tcatcgagag
     3541 gattgagagt aaccaatacg ggcaggcggc cgatcaattc cgggatgagg ccgaacttct
     3601 ggaggtccgt cgggatgatt tccttgagga gcgattccat ggagtgttcg gacttggagt
     3661 ggatgtccgc accgaaaccc atattcttgt ccgtcgtacg gttgaggatg gtcttgtcga
     3721 taccatcgaa agcgccgccg cagatgaaca ggatgttcgt cgtgtcgatc tggagcattt
     3781 cctggtgggg atgcttgcgg ccgccctgag gcggaacgcc ggcaacggtc ccttcgagga
     3841 tcttcaacaa ggcctgctgg acgccttcac cggaaacgtc acgggtaatg gacgggtttt
     3901 ccgatttgcg ggcaatctta tcgatttcgt cgatgtagat gatgccccgt tcagctttgg
     3961 caatatcgta atcagcggcc tggatgagct tgagcaggca gttttcgacg tcttcgccga
     4021 catagccggc ttcggtcagg ctcgtcgcat cggcgatggc aaagggcaca tcgaggaggc
     4081 gggccagggt ctgggccagg agggtcttgc cgctcccagt cgggccgacc atgacgatat
     4141 tcgacttctg gagctctaca tcatcatcag aggcactgaa attggtctgg agccgcttgt
     4201 agtggttgta cactgcaacg gaaagggtct tcttggctgc ttcctggccg atgacgtatt
     4261 cgtcaagggt agccttgatt tcatgcggcg tcggcaggtc cttgagctgg aagtccggcg
     4321 tttcttccgc cttttcttct tccatgatgt gctgcgctac ggcaacacat tcgtcgcaga
     4381 tatagacacc cgggccggca atgagcttct gtacttcgct ctggggccgg ccgcagaaac
     4441 tgcaacgcgg ttcatcattc ctatcgttca tcttgtcttc ctcctgtcct atttcgtttc
     4501 atccggcagc ggacgggtca ggatttcatc gatgaggccg tattccttgg cttcttcggc
     4561 agtcatgaaa ttatcccgtt ccgtatcctt ctggatgacg tcaatgggtt gtcctgtgtg
     4621 ttcagacaag ataccgttca attccttgcg catgcgcagg atttcgcggg catggatttc
     4681 gatttccgta gcctggccct ggacaccgcc gagaggctgg tggatcatga cctgggaatg
     4741 cggcagggcg aagcgtttgc ccttggcacc ggccgttagg agtaccgacg ccatgctggc
     4801 agcggaaccg acgcagatcg tcgagacgtc cggcttgatg tactgcatgg tatcgtagat
     4861 ggccatgcct gccgtgacga cgccgccagg actgttgata tataagtgga tatccttgtc
     4921 aggattttct gcttccagga aaaggagctg ggcgatgatg ctgttggcaa cggtatcatc
     4981 aatcgggccg ccgaggaaga cgatgcggtc tttcaacagg cgggaataga tatcatagct
     5041 ccgttcccct tgtgccgtct gttcgacgac gatgggtaca tacatatttg tcaaaactaa
     5101 cacctccgga gaatgcgttt ccgggattag tcttcagcct ttgcgtcttc agctttagct
     5161 tcttcttttt tgtcttcgtt cgggtcgacg gcatttttca ggatgaagct ggctgctttt
     5221 ttacggccga cagactgtac gagcatggga acgcgatgtt ctttcaaaat gatcttgtag
     5281 acttctttcg gatcggcacc gaagttctga gccatcgtga tgatttcagc ctggagatcg
     5341 atgtctttga cttcgatgtt ttcagctttg gcgatagctt cgaggacgag gtccatcttt
     5401 acgttttctt tagccggttc agcgtaatgt tctttgagtt tgtccatatc ctggttcatg
     5461 tatttgagat aatcgtcgag gctcatgcgc tgtgtttcca gtttcatggc c
//
LOCUS       DAWABZ010000117         5482 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_3840_length_5482_cov_3.647687, whole genome shotgun sequence.
ACCESSION   DAWABZ010000117 DAWABZ010000000
VERSION     DAWABZ010000117.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5482)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3840_length_5482_cov_3.647687"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    772..781
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1107 a         1562 c         1605 g         1198 t
ORIGIN      
        1 atcgtacata ttccagagag ggtgccctac gtggtcgccc ctacaagaaa aggatttgtc
       61 gcacgtgcga caaatccttt ttttatgcct catacagtct cttgcggccc gccctttttt
      121 gctggtataa taaacttgat ggactatgca taaaatttat tttttgccct gcaaaggagt
      181 gttttttatg aatattgcgc aatatacgaa agcggcctct ctggaagaag cgtgggagct
      241 taaccagaaa cggtccgctg tcgtcctcgg cggctgctgc tggctccgct tgtcgccgca
      301 gcggcgcatt gcccaggcta tcgacttgtc ggcgctgggg ctggaccaga tcgaagaagg
      361 ggaggactct ttccgcctgg gtgctatggt cacgttaggg cagctagaag gccacgacgg
      421 gctggctgct ttcactcagg gcgccatgaa ggaagcggtc cgccatatcg tcgggaccca
      481 gttccgcaat atggcgaccg ttggtggcag tatcagcggg cgcttcggct tttccgacat
      541 ttggacgctg ttcctggtcc tggatgccca ggtcgaattg tacaagggcg gcatcgtcag
      601 cctggccgat ttcggtcaga ccgggccggg gaaagacatc gtgactcaca tcatcatccc
      661 gaaaaaggcc tgccagacgg cgtatgattc gctgcgcctc aatgaaacgg acttccctgt
      721 catcgccgtg gccgtatcgg ctgacgcaga aaacgtgcgc tgtgccatcg gnnnnnnnnn
      781 ncccgcccgg cccgggcaga agtcgtccgt ctgccggcag ctgatgtgcg ccgccagggc
      841 ttggaagggg ctgccaaaca aattgcccat tccatgactt atgaaagcaa tatgcgcggt
      901 tccgccgatt accgccagga tatggctgtc gtcctctgtc ggcgcttatt ggcaaacgtt
      961 ctgggaggtg accagtgatg accattacgt attggctgaa tggccagaag gcgacggctg
     1021 acgtcgcgcc gggcctgatg ctctatgatt ttctccgcag ccagggctgt tacagcgtca
     1081 aatgcggctg tgaaacgacg aactgcggcc tctgtacggt ctggatcgac gatgtgccta
     1141 tgttgtcgtg tgccctgctg gctgtccgca tcgacggccg ccatgtgacg accctcgaag
     1201 gtttgcagga tgaagccaaa gaattcgctt tctatatggg ccgcgaaggc gccgaccagt
     1261 gcggcttctg caaccccggt ttcgtcatga acgtcttggc catggtccgc aagctggatg
     1321 cgccgacaga agaggaaatc aaggaattcc tgtcaggcaa tctctgccgc tgcacgggct
     1381 acatgagcca tatgcgggct atcaaggcct atttcaaagc caaggggaag gaggtccggg
     1441 cataatggaa tacaaagcta tcaatcacgt tgtcgccaag aaggattctc aggccctggt
     1501 cacgggccag ccggtctata cggatgatct gcggcccaag gactgtctca tcgtcaaggc
     1561 cctgcgcagt ccccatgcca gagccatcgt caagaaggtg aataagagcg ctgccgaaaa
     1621 ggtgccgggc atcgtctgca tcgtcacggc tgaagacgtg cctcagtcgc gtttcaccat
     1681 tgccggtcag acctatccgg aactgagccc gtatgaccgc ctcatcctgg ataagcagat
     1741 ccgcttcgtc ggcgatcccg tcgctctcgt cgccggtacg aatgaagacg ccgtcgacaa
     1801 ggccctccgc gtcctccgcg ttgaatacga agtccaggaa ccgctcctcg atttcaccaa
     1861 agctctggac aacgatatcg tcgtccatcc cgaagacaac tggaagcccc tcgtcgatgt
     1921 cggcggcgac aacaagcgca atctcgtagc ccaggcctgt gacgaacacg gcgacgtcga
     1981 tgccgtcctg gcttcgtcgg acgtcgtcat cgacaggacc tatcatacgc gtcagaatca
     2041 gcagggcgct atggaaacgt tccgggccta tgcctataag gacgcctttg gccgcctgac
     2101 catcgtcagc tcgacgcagg tcccgttcca tgtccgccgc gtcgtcgcca acgccctgga
     2161 aatcccgaaa tcgaagatcc gcgtcatcaa gccgcgtatc ggtggcggtt tcggctccaa
     2221 gcagactgtc gtcatggaag tctatccggc ttatatcgcc tggctgacgg gcaagccggc
     2281 gtacatggtc tacagccggg aagaatcgat gaccgtatcg acgccgcgtc atgaatcgca
     2341 gatccgcgtc gtcctcggcg ccagcaaaga cggccatatc cgcggcattt tcctcgattc
     2401 cctgtggaat gccggtgcct atggcgaaca cacgccgacg acggtcacct tgtcaggcca
     2461 taagtcgatt cctttataca atgcagccga agccttccgc ttcagctata cctgcgtcta
     2521 taccaatacc attgcggccg gcgcttatcg cggttacggt gcgactcagg ggctgttcgc
     2581 cctggaaacg gctgtcaacg aactggctgc ggctctgcac atgagccctg tcgccctgcg
     2641 cctgaagaac atggtccgcc aggggcaggt cctgccggcc tatttcaatg aaacggcctt
     2701 gagctgtacg ctggataaat gcctggaacg ggtcgtccag atgtcaggct ggaaggacgg
     2761 ctgcccgcgc caggtcctgt ccaacggcca tatccgggcc atcggcattg caacggccat
     2821 gcaggggtcg agcatttcca atgtcgacgt cgcttcggtc accatcaagg tcaatgacga
     2881 cggcttctat tcactcaata tcggagctac cgatatgggg acgggctgcg ataccatcct
     2941 ggcccagatt gccgcggaat gtcttatgtg ccctgtcgac aatgtcgtta cttatggcgt
     3001 cgatacggat acatcgccgt acgacagcgg atcctatgcg tcgtcgacga catacctcac
     3061 gggcaatgcc gtcgtcaaga cctgccagag cctgatacag cgcatgaaag aacgggcagc
     3121 tatcaagctc ggtgcagcca acgttgacga tttggaattt gacggcaaag ccattactga
     3181 cttgtctact ggcaagaaaa tcaccctcaa ggacttgggc aatgacgcca tgttcatgaa
     3241 tgacatcgcc ctggaagcga cagaatcgtg ttattcgccg accagtccgc cgccgtatat
     3301 ggccggtgcg gcaacggtcg acgtcgaccc ggaaacgggg cacatctccc tggtgcagta
     3361 tgacgccgtc gtcgactgcg gtacggtcat caatccggcc ctggccaaag tccagaccga
     3421 agggggcatc gtccaggcca tcggcatggc cctgatcgaa aatatccagt atacgccgtc
     3481 gggccgcctg cagaataact ccttcatgca gtaccgtatg ccgacgcgca tggacctggg
     3541 ccagatcaac gtcgaatttg aaccgagcta tgaaccgaac gggcctttcg gcgccaagtc
     3601 catcggcgaa ctggtcatcg actcgccggc accggccatt gcccacgcca tctataacgc
     3661 tacgggcatc tggctgcgcg acttgcccat gaccgccgaa aaactctaca aggctatgca
     3721 actgtagggg ccgcccaaag ggcggcccgc aggtcgcagg tcgcactccg caatgcggga
     3781 gccgtccggt acggcctgcc ctggctccct ttatagggga gctgtcagca acgctgactg
     3841 tgaggttgct ttttctcaga aatttcaatg aaaaagggct atcgtattaa gtgtttttgc
     3901 ttaatgcgat agcccctttt tatgtattcg ggtgtagtcg gagtgcatta tccccgcctt
     3961 agcaggatgg cccctacgga tacggtccat aagaccgttg cgacctgcgg cctgcgaacc
     4021 acgaaccacg aaccactact aggccgaagg ccgaagccac gagccacgaa ctcctcactt
     4081 cacaatcatg acgggcaggc ccgaatgggt cgtgatgtag cggctgacgc tgcccaggag
     4141 aatcgaggcc aggccgctgc ggccgcggct gcccatgatg agcaggtccg agccgtcttt
     4201 ttcggctttt ttgaggattg tcgggcccgg acggccgatg ttggcgacga agtggatgtc
     4261 gacgccgcct ttatggtcgg caatggcggc cttgcacagg gcgaagacgc ggtcggcgct
     4321 ctggcgcagg cggtcggtca agtcttcgag gccgcgtttt ttctggtcca gtgccgtttc
     4381 ttcggcgatg aggtcgcgca tgttggcgac gtggaagacc gtgaccgatg cgccgtcggc
     4441 tttggcgaat tctacggcgt agcggacggc tttcagtgat tcttccgagc cgtcagccgg
     4501 gacgaggatg cgctggtatg gcccttcttt ttcgttgtgt gccaggagga ccgggcattt
     4561 ggcatgggcc aggacgtagg tgctggcact gcccaggagc tgttccgaca gggaatggtt
     4621 gccccggcgg cccatgacga tgaggtcggc gtcgcatttg tcggcttcgc tgaggatgcc
     4681 gatttccggc gtactgacca gctggaccgt gtggaccgtc aggttttccg gaacggcgtc
     4741 catggctttt tccaggacgt agccgctctt atccttggcc agtttggtca ggtccttgac
     4801 tttttcatcg gtcatgtctt caaagacatc gaagctgtcc aggacggcat cggcattggt
     4861 gatattgacg atggtcaatt cggcactata tgcgttggca atgtcgatgg catgttgcaa
     4921 ggcgttgcgc gacggctggg aaccgtcaat aggaactaag atgtttttca tgatacatac
     4981 ccccttttat tggaactatt tttccagtga gctgttgatg cggacaccgc tcatgaaggg
     5041 catgaccggc ttggtgtctt cctgtaccag ttcgcggatg ccctcaaaag tcgtcgtcag
     5101 catcagtttc aggcggtttg tctgattgac cgagctggca ctgggatcat agtcgatagc
     5161 gacgatattc gtttccggga aggcggcttt caaggtcttg atcatccctt tgcccgtaac
     5221 gtggttcggc aggcaggccc agggctggag gcagacgatg ttcttggcgc cgcggtggat
     5281 gaggtcgatc atatcccccg tgaggaacca gccttcaccg gcttcatggc ccagggacaa
     5341 gtatttcttc gcttcgtcag cgatgtcgta aatggatgtg atcgggtcga aatggcggct
     5401 cttggcgacg gcctgttcgt aaggcttgcg gtaccattcc aaaaggcgga cggcagtctt
     5461 gctgaggaag gaatccttcc ag
//
LOCUS       DAWABZ010000118         5461 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_3853_length_5461_cov_3.274695, whole genome shotgun sequence.
ACCESSION   DAWABZ010000118 DAWABZ010000000
VERSION     DAWABZ010000118.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5461)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3853_length_5461_cov_3.274695"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3808..3817
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1317 a         1505 c         1321 g         1308 t
ORIGIN      
        1 attttgacga aaaacacgaa atccctggca ttgccgccgc tggctgtcgg catgggcatt
       61 tacctgccgc cgacactgga aatccctctg gtcatcggtt cggtcatggg ttatctcatc
      121 cataagtccc tgaaagcccg cgctgcccgc cgcagccctg gccacgaaga agaagacgta
      181 gaagcctgca atcatcgcgg tgtcctcttt gcatcgggcc tcatcgtcgg cgaaagcctc
      241 atgggcgtca tcatcgcctt gctcatcgtc gtttccgtta cttccggcgg cagcgaaaac
      301 ccgctggccc tggtcggcaa agacttccag tctacagctg atatcctcgg cctgctctgc
      361 ttcatcgcca tgatcgccat cttcgtccgc cacatcttga ttaccaagtt caatccggaa
      421 gatgccgaaa gcaagtaagg aatatgccgc ttacattaga atttcattaa aatttcgaca
      481 aaatcctcaa aaaaagtcct cgtacacaat acattttttg gtacgagggc tttttttgca
      541 tatttcttct tttcccctaa cggcgtataa taagggtatc ccgcagacta tcctgcgagt
      601 ccactttaaa ggaggtcctt tctttgttct ttcccaaact cgacaagcaa tccctgaccg
      661 acggtatcgt catcccgcag atggtcaaag tacaccagtc tttcccccat aaagatttag
      721 acgatcccgt gggcacactg aaacagcagc tcctggctct cgatgaaaag acgacggcca
      781 gcctgcctgg taaacgcatc ggcatcacag ccggcagccg cgggctcccg cactacaagg
      841 aaatcatgaa agccctttgc gaccagctca aggcctgggg cgccaagccc tttgtcttcc
      901 cggctatggg cagccatgcc ggagccaccg ctgaaggcca aaaagcccat ttagcccagt
      961 tcggcatcaa tgaagactat ttaggcgtcc ccgtcctttc caccatggac gtcgtcgaag
     1021 tcgctacctt agacgatggc ttcccggtct actgcgataa aaacgcctac gaagccgacg
     1081 gcatcatctt attcaacaag attaaaccgc atacacactt caaatataaa cacgaatcag
     1141 gtctgctgaa gatgatctgc atcggctgcg gcaagcacaa aggcgcttcg accttccacg
     1201 gccggggtta tgacgctttc gggcccaacc tggaacgcgt atcgaaagcc ttcttgcagc
     1261 atgtaaacgt cgttttctcc gtcggcatgg tccagagccc cagcgatgac atcgccgccc
     1321 tggaagtcat cccgagagat aaattctttg aacgcgatgc tgccttgcag accatggcca
     1381 aagccgaaat gccccggctg aaactgcccc acatcgacgt cctcatcgtc gacgaaatcg
     1441 gcaaggaaat cagcggtacc ggcatggacc cgaacatcac gggacggacg gaacgcttca
     1501 gtcaggaacc ggccttccgc gccttagcgc cggacatcga acgcatcgtc atcctggata
     1561 taaccgaggc ttcccatggc aatggtgccg gctgcggtat ggccgacatc gtcagttacc
     1621 gcttcgtaaa caagatggat ttcgcttccg tttataccaa ctgcattacg gccaagtcct
     1681 tccgcatggc cctcctgccc ctgtatggca acagcgataa agacgccatc gaaatggcca
     1741 tggcccacgc cttcctgacc gacccgaacc aggctaaaat cgtccgcata aagaacacac
     1801 tgaaactgga agacatcgaa tgttccgtct cgtgcttaga agacataaaa aacgatccgg
     1861 acatgacaat cacatccgaa ccgtttacat ggaagtttaa cgaagaagat aatctctggt
     1921 aaactagttt ttatgaccga tggcatccat ccaggcgaag agctcgtcta aatcgtaatc
     1981 gccggcatgg ccgcgggccc agggagaggc aaaatcgaca tcataaccgg cctgctgcag
     2041 acgcagggcg acgactgccg gtacggcaat ggtcgtatcg cggtcggctt cgccgtggcg
     2101 gatacgccag taatgcgccg tcttgacgcc ggatttgccg atgtagttca ggggattcat
     2161 cgtcttgacg atgtccttat cggccagagc cgatccggct atggtcgaat ggtctttgcc
     2221 gtatgtatac tgtaaaatgc tgtttatcga cagttgccgt gccgaactct tccgtttcag
     2281 cactgctcat atcgagcgca tcaaaggccg gggctgcctt cagccgggtt acatcgacag
     2341 catagccggc taaatccatg tcggtcactt tgccgtcgtt gatggtcagc catgtcttgg
     2401 aagacaaatc ggtgccgttg tcgagggctt tctgtgccga agccatgtaa atcgatttaa
     2461 tgtaatcggc aaaggagcct cgtccatttt catcgagggt caaggctttc ccattcttgt
     2521 caacgagatg taaactattg atataagccg ggtacatggc tttcaaatcc ttcgatgcct
     2581 gttgctggac agcggtcatt tccgtcccgg ctgcggcttc gaccggtgca ttgtcaggcc
     2641 ggttggtaac gacgcctgcc ggtttagcct ggctattacc tgccggcggt ttcatagcac
     2701 ccatatttcc ctgatgatac gtgtttacac cattaaacat ccattcataa gccatgtcag
     2761 catggtccag gtcggtaatc gggcagtata cgctggaagc ataaatatca tcccgttcat
     2821 cagccgcgcc aagggctttc aaatacggtt catagtcctt actgtcgcct gtcgccccga
     2881 tgagcgtcga taaagcaccg ccggcactgg ttccattaga aatgattttt tccgtatcac
     2941 caggcagttt ccctgcatta tggcgcaaat aacggacagc ggccttcata tcgacgataa
     3001 gtgccggagc cttgccggta tacgtgccgt cagcagcctg agttgtccgt ccacgggctc
     3061 ccggagctgc gacgacatag cctttggaga gggcgacgag catggcattg gcaccgccag
     3121 tcatccgatc attttcagac ggctgccccg gtgcccccgg catataaccg ccgactgtat
     3181 tgggcaggaa aatcggagct gtcttcgccg tatagccgtt gatcgtcttg ccattaaagt
     3241 atccttcggg aacgtatata ttcatggact ggtatgtcgt atcgacagga tgggccacat
     3301 acacccggtt ttcataggcc cggaaagcca ctttcttacc gtcgactgtc atcgttttct
     3361 tttcatactt cgtcgtatcc aaatcgaggc tatacgtctg ctgcaccgga gcagcctggg
     3421 ccgttttagc cttagtctgc gtagccgccg ctacggcttg taccggttct gcttccgtcg
     3481 tatccgccgc caacaccagg ccgccagtca tcatgaaaaa cagcgctgct gcgcacatct
     3541 tatttctacc tcgcatatca caactcacct ttccttaaaa atagttttaa ttaaaaccat
     3601 ttctttctgc tttagtatac catagaaatg tggcgatgct atgacagttt ggaataaaaa
     3661 aagagctgtc ctatgaagac agctcttttt ttagcagtca gcagatagcc gttagcagtt
     3721 agcggatggt tttaaattta aagtctccgt gcacctatca gcccatcagc acttgcgcca
     3781 ccaggtctgt acaaccgtgg ccccgacnnn nnnnnnnata gtaaaggatg tggtcagcta
     3841 gttgtgaaca acgtttacta tggtaaacga catggctggc cgcagctccg atgaagaagc
     3901 cgccgacgac gtcgctgacg taatggagac cgctgagtat ccgcgacgac gccaggacgc
     3961 ctgtccaggc cagggccggc agtccccagg ggttgtgccg ggccagtagc atcagggaaa
     4021 tggccagact gttcatggaa tgattgctgg gaaaggacgc attggcctta tgcgctacca
     4081 gcggcgccgc gtcaccacag gcaaaaggcc gggggcgatg ccagatttta ccgatgaccc
     4141 aggaaaccga agagcccacg atgacggaaa agagacagta aactaatgtc ttccgacgtt
     4201 taatctgctc tgaaaccggc cgcggccgga accattcgat aaggccataa aggatgaaca
     4261 gccagtagcc gaaccgggta atccaggtaa ggatccgaag ccaggtcctg cgccgggcaa
     4321 tgaaccattg aacgaataaa aagatagacc ttgccatgat agcctctctt tcagccggac
     4381 tcagccctgt tttgcaaaga cgtcccgcat cgttcccgct acatcggcca aggagatgct
     4441 gcgcatctta tcctccatgg catcctgtac ctgatgcagc cggtcatcta accctgcgtg
     4501 aatcgtcttg cccaccggac actgcgggca gggatgttcg tggaaatgga agagcgagtc
     4561 gccgccaacg cagtctacgg catggtaaac gtcatataaa ctaatgtcag caatcggttt
     4621 ttccaacgct gtgccaccag tcccgcgctt gacctggatc aacccggccg ctttgagctg
     4681 gatgagcagt ttgcggatga tgacgggatt cgtccctacg ctggcagcaa tgaagtcact
     4741 cgtcactttt tccgttttgc cgaagtaatc gatacacaac agggtatgaa cggccatggt
     4801 aaatcgactg gaaatctgca tatgaaagac tcctcaaatg gcttttaatt cacgaaggat
     4861 agccaggtca gccgtacaag ccgggcagtc acagaattta gcccctttgg cctgaatatc
     4921 tttggcatgc gccaagagct gtttcgctgc atcgtccccc ataatctgga cagcatgttc
     4981 cgaagctgca aagtccagga gttcatcgat ggtcgagacg ccctgcttca aggattcgat
     5041 ataagccggc gcagctgcgt ctttatcggc agcatcgagc caagtctggg cggccgtttt
     5101 cagcgtcgga ttgcagctgg gagctgccat caaatcatgt gtcttagccg taacgaattc
     5161 tactgttttt gcatccattt taaaaaacct cctataaatg acgaatcaaa acatccagga
     5221 tagaattatc ctgattacat ccctatcata cgacattttt attgtaaagt caatggttac
     5281 atattatatg ttttgttgaa ctattttaaa cttatttatt ttgatttaaa tatttgtcat
     5341 ccgcttggtt atcacgtttt taaaacgagc gtattctcta tttaaacgtt tttattcgcg
     5401 tgtaaacaat tgtaaaaaaa gaggctgcgc tcgtgcgaca gcctcttttt gagtattaag
     5461 t
//
LOCUS       DAWABZ010000119         5393 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_3912_length_5393_cov_3.281004, whole genome shotgun sequence.
ACCESSION   DAWABZ010000119 DAWABZ010000000
VERSION     DAWABZ010000119.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3912_length_5393_cov_3.281004"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3697..3706
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1081 a         1281 c         1630 g         1391 t
ORIGIN      
        1 gaaaataaac gcaggccgca ggcggccggt tgcaggtcgc aaaaagcggc cagcggccgc
       61 acgtaggggc tcgcacgtgg cgagcccgct agggagattt cgccacagcc cattcgagta
      121 taatggcagg ttttacgaat tatgagccac gagctactaa caactagcca ctcaaaaagg
      181 cgctgtcttc gtgcgacagc gcctttttac tcacggcata ctgatccctt cctggtggtt
      241 catggagaag ccgccttttt ctacgtcgtc gaagtcgagg gacgggtcgt attctggaag
      301 tttggtcaag cgtttgacca ggccgaaggg ctttttcagg gagagtttat ctcctttgcc
      361 cgagaggacc cagaggactt tgtagccttt gggcggcgtc tggagttttt cttcgccttt
      421 gccgtcggtg aagtagacga cgaggtcgac gcgctgtgta ttggcatagg cgaagaccgg
      481 ggaataggct gtgccgccgc ggatgtccag gcggccgcgg acgtcgtcta tagtgcggat
      541 gcggtaggtg cgacggattt catcgtcaca ttcggcgacg atgatttcat ggttgtagca
      601 gcggaccagg tgcaggactt cgccgatggc ctggcggaat tcggcgtcgg tgatgctgcc
      661 gctgatgtcc aggccgatga ggattttggc cgtatgggag cgcaggcagc ccggcaggtc
      721 cagccgttct ggctggcggc ggttgcggcg catggtcgtc tttttccagg tgctggtgac
      781 ggagccgacg agcttcttca ggtaccagtg ccagggcaga tcttcctggg catctttcag
      841 ggctgcaatc atgctttcca gatagttcga cagttcgcct ttgcatgacg cgtcgatgta
      901 tttttctgtg aatttgcgca gcgtctcttc gtcgatgtcg tcgccttcgt cccataaatc
      961 atgggtcttg gccgggtcgt aggacacggc gatggattcg tcggaatcgc tgtccgattc
     1021 gggcaggtcc ttcttgtcgg tccgcaggtc cagggcgcct tgaatcttat cgacgtagta
     1081 ttccaggctt tcaaagggct ttaatagcag ggcatagttc atgttcaccc attccagggt
     1141 cgtcgagaag ggcggcaggt ggtcgaggta ggtgttgacg acgacgtcca tggccagatt
     1201 gacggccagc ttgctgtatt gctgccgcag ttctttggcc cggatgaggt ggagcgatac
     1261 gacgtggagg atttcgtgct tgatggtgct ctccatctgt tccggcgaca ggggcaggaa
     1321 gagcatgggg ttgaaataga ggtgataccg cgtccccttg aagttggtcc ccgtcggtga
     1381 agccatgtcg aagcggatgt ccttgaccat ttggaagagg aaatagccga agaaattgtc
     1441 tttgtcttcc atcaagtgac cggtgatgcg gtccaggaag gcgtagaatt tcttttggaa
     1501 atcggccggc acgtccggga cgctccctgt ccggcgctgt tcggcgagga cggcgtcggc
     1561 tacgatagat gcctgctgat agagtagatt ggccgcaatg ggcagtgact gttccataag
     1621 ctgttatcgc agggagcgga aggcggcgaa gtagctgtcg atgaagtcgt cgttatccaa
     1681 agcgcaggca tagactttgg ggttggcgga tttcatgtcc ttcatgacgc cgaggcgcag
     1741 gtcgaccggg tagagggcca ggaaggctac gaagcgggac aggagcgcct gggcctggct
     1801 gtcgtcgagg cggagttcat gttccagctg tttaaggata ttcttggcag ccagatagag
     1861 gcgggtgtgg ctttcgctgg caatccggct ggacagggcc ggggccaggg acggtccgcc
     1921 gaagacgtcg tcataagcga tgaggggctg ggcgtcggac gtgacgaaat ggacgaattc
     1981 ctgggcgatg agtttgccga cgttgccgcg gatgacgttg tagaagacgc cctgcgggat
     2041 ggccgacggg tcctgtttat aaatgtagta cagcttggaa atgcgttcgt aactgcgcgg
     2101 cgtcgcattg atatcgtctt cgttggtctt gtccaggtat tccgggaagg tggaaatgaa
     2161 ttccatgact ttttcttcga tgcccgctgt cgaggcccaa tcgagccact gcaggtaatc
     2221 ggcttccata tacagccaga cgaagcggtt ctgctgggcc gcatccattt cgacgacttc
     2281 gtagtcgtag gaatcggccg ggttcatggc ggcgatgata tggactgacg gtgataagac
     2341 gtaaccgttg atttcacggt tgaggatgag gttcatcagt tcctgctgga cggcgtggtc
     2401 gcagcggttg atttcgtcga tgaacaagag gaccgtcttg cccttggcga tttcttcgtc
     2461 gattttgcgg agtttatggt gaacggcgta aatcgtcgtg cccgcttcga ccgtaggcag
     2521 gccgccgatt tccccttctt tgaggaggtt gccgtcgatg ccgacgaggc tccagtcgtt
     2581 cttggcagcc agttcccggg ccagggaggt cttgccgata cccgtttcgc cgacgagcag
     2641 tgggacttcg ccggctttga ggactaattc tacgcttttg aaagtatctg taaagttcat
     2701 gatttcgctc ctatttcaat gtcaattttt catctactgt gatgcggcgg gccttcttgc
     2761 tgccgcaatc gtcgacgagg aggactttat cagctggcgt caggtcgacg gcgctgcagg
     2821 taatgaagtt gcggacgtag tcttccggca cgtcatcccg ggacaagacg gccttgaagg
     2881 tgttgcgcca gtcatcgggc gtcagggaaa tgcccttggg catatatttg ctgaccagga
     2941 cgttgcggct gaggaagggg aggaactctt ctttgctgat gtccgggatg aggcggatgg
     3001 cctgttcatt ttcgctgatg gccagttctt tcgcttcttc tgtcggtttt tcgatgtagc
     3061 gcagggcttc gtaattgccg cggatggcag ccagctggac ggccggtgtc ggatgctgga
     3121 tgtatttgat ggcgtcgtaa ttcttttcta cggcccgcag ctgcaggtct tcgtccgggt
     3181 ctttgacgta tttgatggcc cagccggact gttccagggc ggcgtcgatg agttccggtg
     3241 acggattttt gatatagctc aagttgttcc agccggactg gacggcttgc aggagcatgt
     3301 cttccgaggg atggtcgatg aattggatgg cccgcgccgt gttggcgacg gcggctttct
     3361 ggacggcttc ggaaggatgc ttgatgtatt gcagggccag gccgttcatc tggacagcca
     3421 ggagctgcat ttcttccgac ggattttcga tgcggtcgat tacataggga ttgttgcgga
     3481 tcatattcat atatttttca ttcgtcatgt caggtcccct tcatttcata taataaagat
     3541 tcattgcctc tattatacaa tagctgcggg ccggagggaa atattttaag tggctggtgg
     3601 ttagcagcta gtggccaggg gatggtttta aattttttac catttgctgc aaacttaaaa
     3661 ctcaaaaagg ggcgttcgga catgcgacag ccccttnnnn nnnnnnctcc tataaggaga
     3721 gccactgcga gccactagcc acgagccact aatccttctg cggcgtgagg gcggcaaagt
     3781 gatgcagggc cgtgtacatg tcgcggacgt ttttgaactg gaccgtgtcg atcttgacgg
     3841 catagtctgt tccatcgggc cggtagacct tggtcagatc ggctttcagg tcgtatgtcc
     3901 ctttgcgctt gttggcgtgc agggccgctt tgtattcgtt gatctggtgg atttcctggc
     3961 ccatctggaa gaccaggtcg tcgcggatgc cggcgtattt ttcgacgtac agcgtcaggt
     4021 tcatgttttc gatgctgttt ggatagtcca tggtctgcat ggcccggagg gaatccatat
     4081 cggcatagac gatgccgtcc gggctggtgc tgatgacccg gtaacggctg ggattattga
     4141 ctagggtctg ggaatccatg gcattggctg tcaggcagcc cagggccagg agcagggacg
     4201 cgcagagtgt cgtcattttt ttcatcataa tgattcatcc tttcttgtat ttgataagtt
     4261 aatcgtttat ccttattata ccatgagggc ctcgtctact acaagcggac ggaaacgggc
     4321 cggcatcgaa atcgatggga gcgaagtcgt cccatgtgta tacataaaat gcccactttt
     4381 ttcttgataa aaattttgaa atatcataaa attatcttat ggaaaacgca aagaaaggaa
     4441 agggtgctat gttagatttt cgtatccata catttttgtg cgtttgccgt cacatgaact
     4501 atacccgggc tgctgcggaa ctgcacatca cacagcctgc cgtttctcag catattcgct
     4561 atttagaacg cgaatacggg accaaactct ttagttacaa ggggaaacac ctgcagctga
     4621 cccaggctgg tcacatcctt tggaatgccg tgactgccat cagccatgac gatgccatct
     4681 tgaaagagca tctccgtgat ttgcagcata accgctcctt gtcttttggt gcgacgccaa
     4741 cggctggcgt ggggattctc gataagttgg ctgacctgct caagcataac gacgatatga
     4801 atctccgcat ggaaattgcc gatgctgaaa aactgctcca gtccctggat gatggtgcca
     4861 ttgatttcgc catcgtcgaa agctcctttg accaggggtg ctatgaatcg ctcttatttt
     4921 ctaatgaacc gattgtcgct gtctgcggca caggctatga agcgccggag gaaatgacac
     4981 tgtctgaact catgcgctat cgggtcatcg tccgcgaacg ccatgccggc gcccgccgga
     5041 ttttagaaca taagctggca gaacaggggt acacactgga acatttcccg gcccgctatg
     5101 aagtaggcag cttgtcagcc gtcaaagggc tggcctgccg cgactgcggt attgcatttc
     5161 tatatgaaaa atcggttcag aaagaactgg ctgacgggac gctgcggcgc attgtcgtca
     5221 aggatttttc cgtcatgcac gcattttcat tcttgtggcg caaaggcagc atgtatcgcg
     5281 ctttattcac acagatatac gaccagttga aggactgagt cctatacgtg ctacttttta
     5341 tttctcatac attttgaaag ggtgaagaga acatgttata ttcaacagaa gtt
//
LOCUS       DAWABZ010000120         5387 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_3921_length_5387_cov_3.385409, whole genome shotgun sequence.
ACCESSION   DAWABZ010000120 DAWABZ010000000
VERSION     DAWABZ010000120.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5387)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3921_length_5387_cov_3.385409"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1186 a         1476 c         1423 g         1302 t
ORIGIN      
        1 atgccatcat gcgagattcc tttggctcta tagtttgtag tacggagttt gtagtttgta
       61 gatgatggct ttaaattaaa aaacattcac taaaaactac aaactgcaaa cttagtactc
      121 aaaaaagagc tatcgcctaa gcgatagctc ttttttatgt acaactctta ttcagctgtt
      181 gtgttgttga caacctgttt gcctttatag tagccgcaag tcgggcatac atgatgaggc
      241 atcattgctt cgtggcactg cgggcattct acgtagcccg gtgcggtcaa tttccagttt
      301 gcgcgacgca tcttctgacg agtctgggat aatctgttct tcggtactgc catgttctgc
      361 acctccttaa atcacatggt acgttatttc tagcgctttt ctttggatcg caacaattga
      421 gccaggactt cgagtcttgg gtcaatcttg tcggtaggac aactacaggg tccgtcgttc
      481 aggtttgcac cgcactgcgg acagagtcct ttgcagtctg gccggcacaa aaccctcatt
      541 ggctcgctga ggatcaacgt ttctctaatt gtctccgtca aatcgatgta ctccccatta
      601 tagggcaata cgtcctcggg aagctctgct tccgtaccat agacttcaga cagtgtttcc
      661 ttccgatcca ccgataccgg tgacaggcag cggctgcagg tgtatttgcc agaggtctgt
      721 atggttccct gtaccaccat gtggttacca tcgaaccaat aagccccatc cacaactact
      781 gtggcgtcct tccaaggaag gtcctccact tcacctagct cggcagccgg acattcaaaa
      841 gaaaagggga aagacttccc ggcttctttt agtgcttctt cgacttgtag tttcatattt
      901 ctacctcgac tatactatta tatctatcta cttgggcttt gtcaatcatt ttttccggtc
      961 tggcccgatg aattcccgca acagcgaatt gggcggtacg acggacgagt cgatgccctt
     1021 gaagggcttg aggaaccgca ggagccgccg ggcttcggcg taatggacgc tgcccttgtc
     1081 gatagattcc aggagcggtt cggcataggg cttgaggaca gacagttcat aaggcaaagc
     1141 cgtgttgaaa atcgtatcgg catcttcctg atagggatag atgttctttt cttcgccgcg
     1201 gcggacgtca gcccagatat ccatggtcgc catgggcccg cggtcgcgga actgctggtc
     1261 ccggaccatg cggcggatga tgcgcgtatc cgacgtggaa atgcggttgt ggtcgttaat
     1321 gcgtatctgc gtcaggacgc ccagggaaat cttgaccttt tcatagcgcg gcagcatgta
     1381 ggtcagggaa tcgttcaagg cgtggaggcc ttcgacgacg atgggctggc ctttttccag
     1441 gactgtcggc ttgtcgtcga aatggcggtg gcctgtgacg aaatcgaagc gggccaggtg
     1501 gacttccttg ccctggttga ggtcatcgat ctgctggttg aagagcggta cgtcgatagc
     1561 ccgcagggac tcgaagtcat agctgccgtc gggattcttc ggcgtatctt cgcggttata
     1621 gaagtaatcg tccaaggaaa tgccgatagg ccgcaggccg ttgacgcgga gctgcgtcgt
     1681 caggcgcttg cagaacgtcg tcttgcctgc cgacgacggg ccggcgatga ggacgacctt
     1741 gaccttcggc cggcggctga cgatatagtc ggccagctgg cccagcttct tttcctgcag
     1801 ggcttcggcc atgtcgacca tatcctgtat ggtcccgttc tcgatgtaat gattcaagtc
     1861 atagacgtat tcgcagcgga cgatgcgccc ccattcttca gcatccagga agaggcgggc
     1921 catcttgggg atttccttgt attccggtaa ttcattggga ttgtcgacgg tcggcgtttc
     1981 caggatgacg ccgggcgcat aggaccgcag gttgaagcag gtcacatagc ccatgctggg
     2041 caggagaggc cccatatagt agtcaaaggt ctggccgcac tgataggcca tgatatgggt
     2101 gacgtcgacg tggcgcagga gttccgcttc ccgcatcata tggcggttgc ggcacatttc
     2161 catagccgtt tcggcgctgg cgatgacctg ggaaatgggt tcatcttgtt cgacgatttc
     2221 gtgcatgcgc ttcttgatga tgtccacgtc gtggacggtg acggtgtggc cgatttccag
     2281 ttcgcagtac aaggccttgc gcagggcatg cttgaccagg accttgccgt cgggatagag
     2341 gtctctgacg gcgcggacca ggaggaagat ggccgtgcgg atgatgcact gattgccttc
     2401 catggtattg atgggcagcc agtctacgtc gccgctctgg ccgaccttgg tctggaggcc
     2461 gacacattcg ttattgtata aggccgctgc aatgggcatg tcgtcgtgct ggcaatactg
     2521 ttcacagacc tgctgcagcg tcactccttc cttgacttca taggaattcc ctgatttcac
     2581 atcgtgtaaa gtaatcacag gaatcacgct ccttttcttt cggtaatagc atattcatta
     2641 taccatattt ttgcagcctg cggcagatgc gccgaaaggc tgaacgccta acgcagactg
     2701 tggtataata agaaaaatag gatttcgtgt agtaaggaag gataatctat gagaaaaaga
     2761 gtgcttacct tatgggatgg caaagtcttc ctggccctgg tcgtcctcat gggctgtatc
     2821 ctagcaggga ttgcgtcata tcagcacgtc tatatgaaag cgcccgatta tgccgtctcc
     2881 caggtcatcc gtgccgcccg ggacggggat atggagacct tcagccgcca cgtcgatgaa
     2941 ggggccctga gcggccagtt cttcgatgcc ctcgtgtccc atacgtcgga cagtacggat
     3001 gaattctttt tgctgaatat catccgctcg ccgttgcggg cttctttcat caccacagcc
     3061 gacctctacc tgaactggac gctggccggg agtacggaca atgacgaata tcagaagacg
     3121 gccgataacc tgcagcgcac cttgaagagt gccggcctgg ccgtccccct ggcgggctgg
     3181 cacctcgacg gcgccgactg gagccatgac aatacagtga agctctactt gtacaacgac
     3241 cgcctgggcg cgaccgtccc ctgtacggtc acgctggaac agacgccgtc ccagacctgg
     3301 atgatcacgg gcctggccga cccggaagcc ttcctcaacg acttgcgcga tgtcatgaag
     3361 aaagaattgg cagcctacaa caagccggtc cagcaaaaaa tcgacgccat catgactgtc
     3421 cgcgacgtct cttccaaaat cgtcaccggc cagggccgca ccttcctgcg cctgagctat
     3481 acgccggtct tccgcaagga ccgcaatgac atcgcttctg tcaaagccgt ctacaccctg
     3541 cgccgggaca gcgataaagc cgtcctctat acgtcgcccc tgcgcctgtc gacatcggcg
     3601 gaaaaacgga cccatgagag ccagttcctg ctcaatcccc tcatcccgtc acagtacagc
     3661 ctcatccgtt cgcagaccct ggacggcatg accagcgaac tccacctcac ggccatcacc
     3721 ctcaaagacg gcacagaatt ggcattggcc gacacgttgc cggaaaaata agaaaagggc
     3781 ctgtcgcatg tgcgacaagc cctttttagt ggctagtggt tagctgttag tggttagcgg
     3841 atggctttaa atttactgta gggtcgccac gtggggcgcc cgctctgtga tatgtacgat
     3901 tttattaccc aaccagtttg ctagaagaat cgtccaaatc cctaacgggt cgcccacgcg
     3961 gcgaccccta caaacaaagc ctttatacac taattcccgc ctgaacagat ttcgatgatc
     4021 ttcggtttgg aattttcggc tttcgacagg gaacggtcga agagtttgac gccgacggcg
     4081 ccgatgagga agccgatgat gccgccgatg acagccgtct ggactgtatc gaagccctgg
     4141 gccagggcca ggaaatgacc ggcaaaggcg ccggcggcac agaccaagag gggcatgacg
     4201 aaacagatga aagcgccgat gacgatgttg acatcgcgga attcgaattt gacgtgctgg
     4261 ccgactttga cgcctaacgg gtcgacagcc gtgacgacga tgttttcagc cccttcgcag
     4321 gcgccgcagg cgatgcagtc ggaatggcgg ccgaccttga tttcggccgt gcctttttct
     4381 tttatagaaa taatcgtacc ttcttcaatt ttcataggaa tcccttcttt ctgtgcctat
     4441 tatagcatgg ccttttcatt caatcaatac tattttcgtg tagtttcaaa catagcacca
     4501 tcaggagatt ccccctttat tccatagcgg gcgccccacg ggtcgcccct acaatgcggc
     4561 tttgccgcgt caaattcatc ccatttgtca taaattccgg ctgtctacag gattcacagc
     4621 gggacgcctt aacaggacgt cccctacggt atcgcataga ggcgatgata ataataagca
     4681 ggccgccgtt cacaggttca gaccgcaaaa aaacacggcc tgcggccgtt tcgtaggggc
     4741 ttccacgtgg gaagcccgct gtgatatttt gccacagcct agccgagtat gatggcaggt
     4801 ctttacgaac aacgagctac gccttttctc ggactcagat gcggcttcgc cgctataaac
     4861 tcaaactaac aacgacgctt cggaaaaccc tctgcgcgta gcgggcgccc cacgggtcgc
     4921 ccctactagc cttatggcat acgcctattc cttcaatcat tctattttcg tgtagtttat
     4981 agtcgtcgta aaaaaaggcg ctgtcccatg aagacagcgc cttttttctg taggggcgcc
     5041 cacgtggggc gcccgctcac gcaaatcatc cagttcacaa gtatctcaca tatacctaca
     5101 aactccgtac tacaagcttt aaagagaaaa gagccttgca tgatggcaaa agccttcgtg
     5161 caacggccct tttttagcag tcagctgttg gttttaaatt tttagccacc cactacaaac
     5221 gataacgtca tacaaaaaat tcacgcggtc cgcccgttgg gacggcccct acgagccacg
     5281 agctataacg ttattacaca ggattcacgc gggccgccta gcctctgctg tccgttccag
     5341 caaccatctt gcgccttccg ttggtcggca cccgatgggc tggaaag
//
LOCUS       DAWABZ010000121         5377 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_3929_length_5377_cov_3.058812, whole genome shotgun sequence.
ACCESSION   DAWABZ010000121 DAWABZ010000000
VERSION     DAWABZ010000121.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3929_length_5377_cov_3.058812"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5151..5160
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1221 a         1469 c         1563 g         1114 t
ORIGIN      
        1 ccgctctgcc tatccagact ggacgttaga acaggggtac tctcagggga cgtattacgg
       61 catcccgcag atggtctgca tggatacgct gttttaccac aaagaggatc atgccgtatc
      121 ccgtgtccag acgatgcaac agctttatga agccattggt ccacggaaga cccaggcatt
      181 acttcccgga cgggacgaag gtgtcatcgc tgatttcagc ttaaataatg aaatgaagta
      241 ctataatatt cttcaggacc atacaggaaa ggtcgttccc gtgcaggcca tgggagacgt
      301 tcaagcggat gccatgcagt atttgaaaga attgtacgcg atgacgggca ccgcgacggg
      361 ccgttatgac gatggcaatg aatttacgcg ggctgaatgg tttgctcagg gccatggccg
      421 ggcgttgtat gcctatccgg aagcgatgag tgccattgtc cgcttcgggc agggtaatta
      481 tacagaagtg aaggccattt ccagctgtga acatcccgat gtagcgacgg cctatgttga
      541 ttacgtcagc atctcttccc agattccagt gaataaggtg gaaccggcta aaaaattagt
      601 ggccttattg acttctaagc ctttcatgat ggccgccctg aaaccggctt cgaaagaagg
      661 cgtaccgcag tatctcttag cggctcgtcg ggacgtcatg caggaattgg ctgcgtcgga
      721 cccgaattat cagaagttgt accggcattt atatcgtgct aaatcgtggc atgtcatgac
      781 gggaacaaaa gattttgctg catgggaagc taaagtcggg cccgttatcg aaaaagggtt
      841 gaaaaatcag taaagtttgg taatcaaggt gtggctcata ccgatgcaga aatcctccat
      901 atcatggaga atccgtctca gatttgaaag aactgcacga aggtatagcc taaatggagg
      961 ccgtacagca tgaggacggt gccgcagagg acgttgatgg tgcgcaggat gcggggtgtc
     1021 atggcgtggc ggaaggcggc gacgcagccc gtgagcgaga ggaaccacag gcccgatgcc
     1081 aggaggacac cggtcaggaa ggtcgtggct tcggcggcag gcagggtgat gcggaaggcg
     1141 ccgaggagca tcgtgccgtc taaaatggcc tgaggattca tccaggtgac gatacaggct
     1201 ttggaaacgg tctggcccag agaggccggc tgacctttaa gggtcagcgc atccggtacc
     1261 tggcgaatca tgccataggc catgtagagg atgagcagcg tacctgcgcc cagaatgacc
     1321 gctttgagcc agggaagatg atccagcagg ctgccgatgc cgtaaaaaca gctcagcgac
     1381 agactgatgt cgaagaacaa gacgatgagc gctgtcacga cggcgcggcg cagggaatgg
     1441 gataacgctg aatggatgac gaagagattc tgcatgccaa tgggagcgac gtaagccagt
     1501 cccatggtca ggccttggaa aaagaacata aaaaagcctc cttcttttcc aaccaaagaa
     1561 agttggttcg tatggtataa tcacagtata acagagctat gccggattcc aaccagccaa
     1621 agcgatgtga attataccaa ccaaaaagga gacgatgtga tgaagcggca tattcccgtg
     1681 caaatcgaat ggcatcccga cccggcgcag gccgtgcccg tataccggca aatcctcgaa
     1741 tggatgtatg gaaaaatgga aagcggtgaa tggccggccg ggacgcgcct gccgtctcag
     1801 cgcgagatgg cccggcattt ccaggtgaac cggagtactc tgaaccaggc tctgaagccg
     1861 ctattggaag cgggcgtact ccagggccgg ggcagccagg ggacggtcat agccagtacg
     1921 gcctggtccc tgcgcctgcc ggttcagccg gattggaacc gttacatgac ggctggctat
     1981 ttccgggcca atcaggaagt cgtccaggcc atcaataccc tggagttcga tgaccgcctg
     2041 gtccgtctcg gtacgggcga actggacccc aggctgttcc cgcaggatgc cttccgccag
     2101 actttgcggg atgtggccga tgccattacc tccctgggct atgtcgagcc gttggggatg
     2161 cccgccctgc gtcaggctct ggctgggtat gccagaaagc ggaattcaat cgtggcgccg
     2221 tcgtccatcc tgattacgtc tggcgcccta caggggctcc agctcatatc ggcagggctt
     2281 ctccgcggcg gggcgacggt ttacgtcgaa gacccgacgt atgtcaaatc gctccaggtc
     2341 ttccagtctg cccatcttcg cctctgcggc ctgcccatgg acgaccaggg cctgtcgctg
     2401 gaagccctgg aaaagcactt gcgccagtct tcagataggg gcaacagcgt cctctatacc
     2461 atcccgacga accacaatcc gacaggccgg accctaagcc gccgacgccg catccagctc
     2521 ctggaattat gcagccgcta tgacctgccc atcattgaag acggggcata tgaagaactg
     2581 acctttgacg gaacgccgcc gccatctctg aaaagcctgg accctggcgg ccgggtcctc
     2641 tatttgggga gtgtttccaa atctttggcg cccgggctgc gcatcggctg gatgatagcg
     2701 ccggaaccgg tcgtacagcg tttgggcgac gtcaagatgc agatcgacta tggcgccagc
     2761 tccctgtccc agcaagtctt ggcccggttc ttgaccagcg gccgttatga acagtatctg
     2821 gatgggctgc gctgtgaatt gcagcggcgc tgccaggcag cctgtcagtc cttgtccgaa
     2881 gtctttgcgg acatggcaga ctggcaggtg ccgcagggcg gtttttatat ttggcttacc
     2941 ttccgcagca gacttcccct ggaactgctc ttccgcaaag ccgtatcggc tggcgtcctc
     3001 atcaatcccg gcgatatcta ttccagccgc cacacccagt ctctgcgcct gtcctatgcc
     3061 tatacgacgc cggaagaatt ccgccaggct gtcagtcagc tggctgtggt tgtccgttcg
     3121 ctggaaagat gaaagatacc gtgaagcctt cagccttgca gtttcagcaa aaaattcgta
     3181 tactggatta gtggctagtg gctaactaat cgataaaaaa gggggattat atatatgaaa
     3241 ttattggaac atgccgtcat cgccgggatg cccgtgcgca atcgcatcct ccgcagtgcg
     3301 acgtgggaag ccttggccga tgccaagggt tatatgtcgg aaaaacagta ccgcatctac
     3361 gaccagctgg ccaagaacga agtgggcctg atctgtacgg gctatgcccg cgtcgatggg
     3421 gaagaccggc ccaacgccgg catgatgggc atttatgacg atgcctttat cccgcagtat
     3481 caggagttaa cccggcgcgt ccatgcctat ggggcgaaga tcatgatgca gatcgcctat
     3541 ggcgggacca agacgacctt tgagcgggaa gggcggacta tcttttcgcc gtcagccgtg
     3601 ccggaacgaa agaccgggac tgtcggtgtc gccatgagtc cggccgatat ccagcacctc
     3661 atcggcgaat atgcccaggc ggccaagcgc gtccgcgaca gcggattcga tgctgtcgaa
     3721 atccacggcg gccattcgta cctgctcaat cagttcctca gtccctatta caaccggcgc
     3781 tgcgatgcct atggcggttc gccggccaat cgctaccgta tcgtcggcga tatcatcgcg
     3841 gctatccgtc aggcagcggg cccgcatttc cccatctgga tcaagattac ctgttccgat
     3901 ttctttgaag gcggcctgac ctttgaagat tcgctggata tctgccggcg cctgcagcag
     3961 tctggtattg acgccatcga agtgagcggc aatatccacg gcaaggccca gcaggaaatc
     4021 ggcctggaat gggatggcca cgtcatccgc gacggcggat atttcctcga ttatgccaaa
     4081 gacatagccg acgccgtacg cgtccctgtc tatgtaacgg gcggcttcaa ggacccgcgg
     4141 cagatggaaa cggccttgaa gacatcgaaa ctggccggta tcggcctgag ccggccctta
     4201 ttatcggaac cgggtttggt ccgccgctgg aaccagggcg atttgcggcc ggcccgctgt
     4261 gtccactgct cgaaatgccg gacgccggaa ggcaactact gtacggtctt tgccgccatc
     4321 gatgccaaga aaggggcgaa ataaatgaaa ttcttcattg cttcagatat ccacggctcg
     4381 gcttattact gccgccgtat gctcgacgcc tggaagcggg aaggggctga ccgcctggtc
     4441 ctgctcggcg atatcctcta tcacggaccg cgcaacgacc tgcccaagga ctatgcgccg
     4501 aaggaagtct ttgccatgct caacgcagtc aaagaggcca tcctctgtgt ccgcgggaac
     4561 tgtgatgccg acgtggacca gatggtcctg gaatttccca tcatggccac ctatgccatc
     4621 ctgacccagg gcagccgcct cgtctatgcg actcatggcg accgctacaa tctggcatcc
     4681 ctgccgccca tgcagcctgg cgacatcctg ctccacggcc atacgcacgt cccggtctgg
     4741 cagcccttcg gccaggataa ctattacttg aatcccggct cgacgtcgct tcctaaggaa
     4801 aacagcccgc acagctatct cctctgggac ggggacacct tcacctggaa ggacatggac
     4861 ggccaggtct ttcaccagct cgtcttatag agagcgggaa gggcaggctt ttgtctgctt
     4921 cataacggcg gaataaggca gccgtatgcg tctctgggga tacgggaaac acaacggtgc
     4981 caaagcggcg gaccggcatg agtcccatga gggagttcgt caggaaacat tcgtcgaaag
     5041 ttcggatatc ttccggatac aagggctttt cgatgacatc atagcattga cagagataag
     5101 accggatgat gcctggcaat aatccggctt cgacgggcgg cgtatagagc nnnnnnnnnn
     5161 ccgtttttga tgaaaaagag attgctgaca gcgccttctg tcaattggcc ctgcatattg
     5221 cagaaaatcg gttcgtcaat accttcggcc cgggcctgcc gtttggctaa gatcgagtcg
     5281 ccataattaa aggtcttatg ccgtaccagt ggggacgttt cattgcggcg aatagacgac
     5341 agggcgatga cataacctgg acggtcagcc atagatg
//
LOCUS       DAWABZ010000122         5342 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_3955_length_5342_cov_4.032911, whole genome shotgun sequence.
ACCESSION   DAWABZ010000122 DAWABZ010000000
VERSION     DAWABZ010000122.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3955_length_5342_cov_4.032911"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3493..3592
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1290 a         1402 c         1413 g         1137 t
ORIGIN      
        1 aatccctatg aagatgggaa ataatttaaa agtgggtaat agaggatgat gatattacga
       61 tgaaaaacgt cagtgaactt tatacaaaag ccaaacatcc cgtccgtatc ttacagttcg
      121 gagaaggcaa tttcctacgg gccttcgtcg attacgccgt cgatgtagct aatgaagaaa
      181 atggttttga tggcagcgtc gctgtcatca tgccccggtc gggcaagacg gaacgctttg
      241 ctaaacagga tgatgtttat acggtctgcc tgcgcggcca gcaggatggc aaagtctata
      301 aagaaaaccg ggtcattact tccgtccaga ccgtgctcag cgcccgcgat gaatatgatg
      361 cgttcatgac catggctcac gaagattcgc tggaattcgt cgtatcgaat acgacggaag
      421 ccggcattac ctttgatgaa aaagatgctt ttacagattg cccgccgtcg actttcccgg
      481 caaaattgac caagttcctc tatgaacgct atacctttta taaaggcgct gccgataaag
      541 gcctgaccat gttgccgaca gaattgaatg acaacaacgg taaactcctt cgggactgcg
      601 taaacaaata tgtatccctg tggaaactgg aagcgtcttt ccagtcctgg ctcgaccagc
      661 actgccagtt cacggatacc ttggtcgacc gcatcgtcgt cggctatccg gctgattcca
      721 tcgcggctct gcaggacgaa ctgggctatg aagacagcct gctcgaccag gccgaacctt
      781 tctccttatg ggttatcggc gacccggagc tggcttcgcg gctgccgtta aattcaagta
      841 aattccatgt tgaattcacg gaccatatcc aggcctttaa agaacaaaaa gtgcgcatcc
      901 tcaacggggc tcatacgtct atggtcctcg gtgcttatct ggccggtctc gattatgtcg
      961 gccagtgcat ggaagacccg gtcatccgca agtctctgga ccagaccgtc ttcgatgaaa
     1021 tcgttccgac cgtccatttg ccggaagaaa aggccgttgc ctttgccaag gccgtttatg
     1081 aacgctttga aaatcctttt gtcaaacacg cattgctctc gatttccctg aattccattt
     1141 ccaagtggaa agcccgcatc ctgccgacct ttaaagacag tctggcagcg acggggaaac
     1201 ttccgaaatg gctgacctat tcctttgccg ctttcctggc tttctatcgg acgacggagg
     1261 ctggtgaaca ttgtcttatc gggtcacggc cgggcaatac ttatgaaatc cacgatgatg
     1321 aagataaact ggcctttgtc aaagaacagg ccggtaaaac gacggctgat tacgtcaagg
     1381 ccatcatggc acgcagtgat tggtggggcg aagacctgtc ggctatcgac ggctttgccg
     1441 atgccgttac ggctcatctg gaccgcatgg ctgaaatcgg tgtcaaagct cacattgccg
     1501 aattgacaaa ggagtaaacg atgaacgtat tccaaatcaa ctctcatgat aatgtcgccg
     1561 tagctgtcga tgccgttgcc aaaggcagcg tcgtcacggc tggcggtaag actttcacgg
     1621 tctgccagga catcccggca gggcataaag tggccctgca gccgattgcc aaaggcgaag
     1681 acgtcatcaa atacggcttt cccatcggga cggctaaaac ggatatcccc gtcggtgcct
     1741 gggtccatac gcagaacgtc caaagtaaac tcggtgattt acttcactat acctatgaac
     1801 cggaaaaagc tgatcgttct ccctggaaaa gtgaaaaaaa atacgaattc cagggctata
     1861 aacggagtga tggcagtgcc ggcatccgaa atgaagtatg gatcatcccg accgtcggct
     1921 gtgtcaacaa tattgccaga gccattgaaa cggcagccca ggcttataag acggaaaata
     1981 tcgatggtat ctatgcctat agtcatcccc acggctgctc gcagttaggc gatgaccagg
     2041 tccatacaca aaagatacta agtgggctga ttcacaatcc caacgccggt gccgtcctgg
     2101 tcctgagcct gggctgtgaa aacaatcagg tcagcctgat gaaagaagtc atcggcgact
     2161 atgatccgga ccgcgtcaaa ttcctggtct gccaggacgt ggaagatgaa attgaagccg
     2221 gcacggctat cgtcaaagac ctctgccatt acgcggctca gttccatcgc cagccctgcg
     2281 atgcatcgct gttgacgatt ggcttgaaat gcggcggctc cgatggtttt tcgggcatta
     2341 cagccaatcc cctggtcggg gagatttcca accgcctcat cgcagccggc ggtacgtcga
     2401 ttctgacgga agtgccggaa atgttcggcg ccgaaacgct gctcatgaac cgggctcgca
     2461 atcgggaagt cttcgacaag accgtcgacc tcatcaacca tttcaaagag tattttatga
     2521 gttatggcga aaagatcaat gaaaatccat cgccaggcaa caaggccggc ggcatcacga
     2581 cgctggaaga taagtccctg ggctgtgtcg agaagggcgg ccgagctctc gtcgaagatg
     2641 tcctggccta tggagacagg gcgacgaaaa aaggcctaaa cttgttgcag gctccaggca
     2701 atgatttagt ggcctccaat gcgctggccg ctgccggagc ccatatggta ctgtttacaa
     2761 cgggccgggg gacgccgttt gcctgccccg taccgacgat taagatttcc agcaatagtc
     2821 acctggccgg tttcaaacgg aactggatcg atttcaatgc cgggaccatt gccgaagggg
     2881 aatcgcggga agccgcagca gaccgccttt tccagtatat tcttgacgta gcgtcaggtc
     2941 gtgtccatgc gaaatcggaa gccttggaca agcacgaact ggctatattc aaaaacggag
     3001 ttactttata gtaaatatgg ataaaccgtt acattcagat gtgacggttt atccatatct
     3061 tgtggaggaa aaacatgagt aaagtgctta ctatcggtga agccatgggc ttgctgattg
     3121 ctgaagaaat ggggccgctg gaagaagtgg aacatttttc ccgccatgtc tgcggggcgg
     3181 aactgaatta cgccgtcggc atggcccggc tgggcaatga tgtgtcgtat atttccaagg
     3241 tcggcgtcga ccccttcggg aagtgcatct gcaacttcct caatgccaat ggcatccacg
     3301 atgactatgt ctggaccgat gacgaccata tgacaggttt ccagatgaag gaaaaggtcc
     3361 tggaagggga tccgatggtg gccaatatcc gcaaacacac ggccttttcc cacttccggc
     3421 cggcggacct ctccggaatc gactggaccg gcgtcgacca cctgcacgtc acggggattc
     3481 ccctggccct gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatcgactg
     3601 gaccggcgtc gaccacctgc acgtcacggg gattcccctg gccctgtcgg aatcgtgccg
     3661 ggaaacgatt tatacgctga tgatgcgggc tcacggtaag ggcgtgcgta tttcctttga
     3721 ccccaacctg cggcccatgc tctggaaatc ccaggaaatc atggcccgtg tcatcaacga
     3781 cgccgcccag tatgccgata tcgtcatgcc tggcctcaat gaaggccgga tcctgacggg
     3841 aatggacaat gaaagagata tcgccggcta ttatttgcag cgcggcgttc agactgtcgt
     3901 catcaaaatg ggcggcagcc atgggaccta tatccgcacg gccgatgacc aggaataccg
     3961 cgtgccgagc tatcacgtcg accacattgt cgatactgtc ggtgccggtg acggctttgc
     4021 cgtcggcttc accagtgcca tcctcgatgg actgagcctg gaagatgctg cccgccgggg
     4081 cgctgccatc ggggccatgg ccatccaggt cgccggtgat aacgaaggat tgccgacgcg
     4141 ggaacaactg gcggtattcc aacagaacgc ataatattgg ttgtgggatt cccacaaacg
     4201 ggccgccctt tgggacggcc cctacgtgcg atcctatggg accgctgcgg cctgcgacct
     4261 gcgttaatga cacctgcatc aatccgtata attggtgcaa aacaacccca ttaaccgcga
     4321 ataggacatg gccgataaat gtggaacggg cgccccacgt ggcgccccta cgctgcggcc
     4381 tgcgttgatg ccacctgcat caatccgcat gattggtgca aaacggcccc attaaccgtg
     4441 aataggacat ggccgatcgg tgtggaacgg acgccccacg tggacgcccc tacgaaaatg
     4501 ggtataaaaa catgtagggg ctcgcacgtg gcgagcccgc ccggaatgat gggggtaaaa
     4561 cgttcatccg tcgtgaaaag gagagaaaat catggataaa ttagatgttg ttaagaaaat
     4621 tcaggaaatc ggtgtcgtcg ctgtcgttcg aggcaagaca ccggaagaag cgatgatgat
     4681 gtccgaagcc tgcatcaaag gtggcgtcac ggccattgaa ctggctttca cgacgccgcg
     4741 ggctcatgaa gtcatcgaag ccctttccaa gaagtatgcc gataatccag acgtcctcat
     4801 cggcgccggt acggtcctcg atgccattac ggcccgcgtc gccatcctcg acggcgccca
     4861 gttcatcgta tcaccggccc tggatataga aacaatcaaa ctctgcaatc gctatcgcgt
     4921 ggccgtcatg ccggggacga cgactctcaa cggcgtcatt acggccctgg aatacggcgc
     4981 tgacgtcgtc aagatcttcc ctggggaaat cctgggcatg aaagccatca aggccatcca
     5041 cggtcccctg ccgcaggctc cgctcatgcc gacaggcggc gtcaacgtcg aaaacgccgg
     5101 tgactggatc aaagccggct gcgtcgccgt cggtgctggc ggtgccctca caggcggcgg
     5161 caagacggcc gatgaaatca cggctacggc caagaaattc atcgctgccg tccaggctgc
     5221 tcgaattaaa agtttatagt atggagtttg tagtttgtag aaatatggtt taaattttta
     5281 cccgtccgct acaaactaaa aactgcaaac ttaatactca aaaagaggct gtcgcacgtg
     5341 cg
//
LOCUS       DAWABZ010000123         5327 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_3966_length_5327_cov_3.272572, whole genome shotgun sequence.
ACCESSION   DAWABZ010000123 DAWABZ010000000
VERSION     DAWABZ010000123.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3966_length_5327_cov_3.272572"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4079..4088
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1121 a         1507 c         1478 g         1211 t
ORIGIN      
        1 ctatgcaggt caccaccgat gctgtccagg tattcggcgg ctatggcttc actcgcgaat
       61 atccggttga acgctacatg cgcgatgcta agatcactca gatctacgaa ggcaccaacc
      121 aggtacagca gatggttatc tctggcgcca tcctcagata gtcaaagagt ttcctaacag
      181 aactctcctc cttttgaaac acgtatagaa atagtataaa acagtatgag ataaaaccgc
      241 ttacaaagag aggctgtcgc cgatgcgaca gcctctcttt ttcgtatttg taatttgcag
      301 tttgtagtgg ctcccctgag aaggggagct ggccgctcca ggcggtctga ggggccttac
      361 tacagtaatg cgtaagccag cccctaacca ctagccacga gccactaacc acttaacggg
      421 gctttacact gcggcctgcg gcgcccgtat gggcgcccct acaccaacgc ccggtaacat
      481 ttctccgaca tgggcacggg cacgccgtac tggcgggcca ggcggatgag ggcgccgctg
      541 aaggtatcca gttcgctctg gtggccggcg gcgatgtccc gctcgaggga gctcgtggca
      601 tccatgtcgt cgctgcctaa gatgcggtcg tactggtcgt cgacgatgtc gtcgggcagg
      661 gggacgccgg cggccttgcc gacgtcatag gcttcctgca ggagagcccg gaattcttcc
      721 ctgccatgga gctgggagag ggcgccgccg atggatgtcc gatagtacgc cgtcaggacg
      781 ttataagcgc agttgaggat gaatttgcgc cagatttcga cggtgatgtt ctcggcgatg
      841 cagcaggtca ggcctggatg gttgaggatg ccgtagactt tcttcgtcgc atcggcgtcg
      901 tccgagccga tgtaaatacg ggcaaagcgc ccttcctggc ggatggagta gtcttcttta
      961 taagatgacg tgatgtagat ggccgaatcg acgatttttc cgcgcttcat gagggccgct
     1021 gccgtatcgt gataggtgaa gccgttgagg acagggacga cgatcgtatc gtcgccgatg
     1081 cagggcagga ccgccgtcag agccgcttcc agggaataat tcttgacgca gacgaagatg
     1141 acgtcctgga tgcctgccga ggcgggatcg tccgtgacgg ccggatgggc cgtgtggtcg
     1201 ccgaagaagt cgctgtggac gacgaggccc tttgtctgga gcgattcttt gcgcttccgc
     1261 cgggcaatga gtgttacgtc atcataattg gcacagagga cggacgcgat atatcccccg
     1321 acgccgccga tgccggtaat catgattttc atagtatcaa cccctctttc gataataaag
     1381 tacaccttat tatcgcacag ggctggataa acggcaaata ggggagggta tgcctaaaaa
     1441 gtatgtgtag ataacatatg aacgcttgaa catatttaac tttaatggta tcatgaaggc
     1501 gaataaaaag tgagtccctg aaggaggcat atctatggca gaagaatttt tgaaagacca
     1561 gggctgttcc tgctgcaacg gccatcacga cgaagacgaa gccgtcaaaa tggatgctgt
     1621 cggcacttgc ggctgctgcg gtgacgacga tgatgacgaa gaagaaagtc ccttgtggac
     1681 cctcgtcgcc gggacggtgc tctttttagt cacctacctg gccggcatca ttcccgatga
     1741 tttttccctt cccgcctata tcatcgccta tctgctctta ggctacggcg ttctgcggga
     1801 agctggagaa aacatcatga agcgccagcc tttcgatgaa aattttctca tggccgtggc
     1861 taccatcggc gcctttgcca tcggtgacta tccagaagcc gtcgccgtca tgctcttcag
     1921 ccgcatcggc gaagccatgg aagaccgggc cgtcaaccgc agccgcaagc aggtggccgc
     1981 cgccatcgat atgcgcccgg aagtcgtcca tcgcgtaacg gccgatggct cgacggtcac
     2041 catcccggcc aaggacgctg ctgccggtga tgtcgtcgtc gtccgcgtcg gtgaccgcat
     2101 ccccttggac ggtgtcgtca tggccggcga aagcctcatc gatacgtcgg ccctgacagg
     2161 ggaaccggtt ccggtcatct gccgggaagg ctcggaagtc ctgtcgggca gcatcaatac
     2221 gtctggcgtc ctccacgtca aagtgaccaa gcccctggca gaatcgatgg tcacgaagat
     2281 tttggaatcc gtcgaacggg cagcggcgcg caagccgaaa atcgaccgtt tcattacgcg
     2341 cttttcccgc atctatacac cggctgtcgt cgccatcgcc gcggctacgg ccatcatccc
     2401 gtccctggtg accggtcagt ggcactactg gatttatacg gctttgactt tcctggtcat
     2461 cagctgcccc tgcgccctgg tcctcagcgt acccctgagc ttcttcgccg gtatcggtgc
     2521 ggcgtcgaaa cagggcatcc tcttcaaggg cggtattgcc atggaacaga tgaagcacat
     2581 cggcgccgtc gtcttcgata agaccggtac cattaccaaa ggtaacttcg tcgtccacca
     2641 ggtcgttccg gccgataacc gcagtgccga tgacgtcctc tgcctctgcg cgtcggcaga
     2701 acaggcgtcg aaccatccca ttgcccgcag tgtcgtcgcc caggcctgtg aacagggcct
     2761 gagcctgacc gagccgtcgg aaatggaaga aatcgccggg cagggcctga aagcccaggt
     2821 cggcagccat agcgtcctct gcggcaaccg ccagctcatg gatacccgcc acatcgtcct
     2881 gccggccatg gacgacgtcg agtacggctc ggaactgtac gtcgccatcg atggggccta
     2941 tgccggccgc ttggtcattt cggacaccat caaagaagac gctgtcgacg ccgtcaaggc
     3001 tatttcccgc atgggcatca cgacggccat gcttaccggt gattctcaga aagccgcaga
     3061 ttacatcgcc tctaagacgg gcatcgacga cgtccgggcc aagctgctgc cgcaggataa
     3121 agtcaatcag atggatgccc tgcgtaagca atacgggccg gtcctcttcg tcggcgacgg
     3181 catcaacgat gcacctgtcc tggccggtgc cgatgtcggc gctgctatgg gcagtggtgc
     3241 cgatgcagcc gtcgaagcgg ctgacgtcgt cttcatgcag tcccgggtca gcgccgtacc
     3301 ggcagcactc cacctgtccc gggaaaccat gaagattgcc atggaaaacg tcgtctttgc
     3361 cttaggcgtc aaaggcgtcg tcatgattgc cgggtttgcc ggttatgctt ccatgtgggc
     3421 tgccgtcttt gccgattccg gcgtcgccgc cctttgtgtc ctcaattcgg tacgcatgct
     3481 ttacaaaaaa taaagtcttt ctggaaaatt ggtaataaaa acgtttaact aaattgaaaa
     3541 tataacgttt aacgaaagaa aatttaaaaa tattaacaaa agtccttgac aaccagcctc
     3601 ttttcaggga taataggcac atcacacaaa cagagcactg tgaaagcagt gcggatataa
     3661 tgacgatgaa cgggtaaaag acgaatcctg atcctcttca gagagccggc ggtgctgtga
     3721 atccggcgag gtgcaatcgt ccgacttcgc ccgggagttt ctgtactgac tgtttttcta
     3781 gggaattgag gtacagacgc aatcccgcgt tataggatgt ctgtcaaaga cgttgagtat
     3841 aatgacaggg tggtaccgcg aatcatcgcc cctgttgctc cactggagca gcaggggttt
     3901 tttatatcct ctgagtgtgc ctcgtacagt attcattatt aaattatttc ttgggtattt
     3961 tggtattcat gtaaagaggt attggctatg gaaaaagcaa tgggtaagat catgaccgct
     4021 gcagctgaaa cgcgggctgt tgccggcacg atgacaaact tccgatggaa aatcgcgtnn
     4081 nnnnnnnnat aagtttcgtc gcttacatgg accgcgtcaa cctttccgtc gcgacgccgg
     4141 tcatcatgca ggaattcggc ttcacgaaaa tcgacatggg cttcattcag acctgtttct
     4201 tcgccagcta cgctttgatg caggtccctg gcggtatttt ggctgaaaag ttcggtatgc
     4261 gcaagaccgg ggctttggcc atcctctggt ggtccgtctt cacggccttg acggctttag
     4321 ctaaaggcaa gatcagcttc gccgctgtcc gcctggcttt cggcatcggc gaaggccctg
     4381 tctttccgtc cttaggggcc gctaccttta catggttcaa taaacacgaa aaaggcaagg
     4441 ccagctcgtc catcctgtta gggaccttct tcggccccgt cgtcggcccg gtcgcaacgg
     4501 tcgctctcat ggggctcttc ggctggcacg ccgtcttcat catcttcggt ctcgtcggcg
     4561 tcgccctggc ctgggtatgg caccgcttcg cctgcgacaa tccggcagac agtccctatg
     4621 tcaatgctga agaagctgca tacatcaacg aaggccgttc ggctgactcc ctgaagaaag
     4681 ccgtcgctcc ctggcataag ttcctccgct cgactcagtt ctgggctgtc ggcatccagt
     4741 tcatggtcgt cgactacatc atgtacgtct tcctggcatg gctgcccatg tacctgactg
     4801 aagttcacaa catgtccttg aaatccatgg gcttctgggc ttcctgccct tggatcgcac
     4861 tgatggctat ggtcttcctg gccggcttcg tttccgacaa gattgccaat ggcagccaca
     4921 gcgaaatgca gtataagatg cgcaccgtca cggctatggc cggcgtcgcc gttacatccc
     4981 tcggcctgta catcgcatcc catacggctg acccggccat gaacatcttc tggatgtccg
     5041 tttccctcgg tgccttgggc tttgctatga gcgccagctg gtcttccgtc atctccctgg
     5101 gtggtaaata caccggttcc gtttccggct ggatcaacct ctggggcaac atcggcggcg
     5161 tcctggctcc tatcgtcacg gccttcttcg tcaccaacta cggctggaac aacgccttca
     5221 cggcaacgtc cctgttcggc atcatcgcca tcgtagcctg gatcttcgta aaaccgggca
     5281 aacaactcgt cccgcaggat aagtgattga tgggcgccca cataggg
//
LOCUS       DAWABZ010000124         5209 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_4059_length_5209_cov_3.173263, whole genome shotgun sequence.
ACCESSION   DAWABZ010000124 DAWABZ010000000
VERSION     DAWABZ010000124.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4059_length_5209_cov_3.173263"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1045 a         1468 c         1433 g         1263 t
ORIGIN      
        1 tcgcccggcg caccgaatag gacgggcagt tgcctgtcga attgagctgg tcgacgatgg
       61 tatagatatc gatgccggcg cgggcactga tggaaatcat gcgcgacaag ccgatcataa
      121 agttgttgca gccgcctgtc gagcccttgc tgaggtatgt ttccaggaga gcccctgtct
      181 ggggatcgaa gaaagccatg cagtgcaggc tgccgcaacc cgtgatgagt ttacgtttct
      241 tgccgatgac gttgtcgtcg atgctgacga tttcaccgcg ttccaggcct tcaccagccg
      301 taggtacacg tttttcttct tcatgggtcg tcaggatacc ggcccgcttg cagccgtcgc
      361 ggaacagggt caggccctta cagccctttt cataggccaa gaggtagagg tcttccgtat
      421 cttcgacggt aaagctgttg ggcacgttga ctgtcgaact gatggacgca tcgatgtgct
      481 gctgccaggc tgcctgcatt tcgacgcgtt catgataatc cagggtcagg gccgtgacga
      541 agtattccgg aagctggccg tcgtctttga tgtggtactt cttcatatag cgttcgacaa
      601 taggcgtata gaccttataa tagacgtccg tcccgtggag ggattccgtc ttgcgttcat
      661 agaagttcgc ataaatcggt tcgatgccgc cggaaatgcc gagcatcgtc gacaaagtcc
      721 ccgtcggggc gatggtcagg agctgggaat tgcgcaggcc gtattccttg accagtttgg
      781 ccgtcgcttc cgtcgtgttg gctttgaaat aaggcgtact catgatttca tcgatatggc
      841 atttttcgaa ggcaccgcgc tgatgagcca gttctgccga cgcagcgata gccgtatcgg
      901 ccatggcaaa gccgatatca tgacagaagg cgacggcgtc tgtgctgcca taagtcaggc
      961 ccatctggat gagcatgtcg ccgaggccca tgatgcccag gccgatctgg cgccaggctt
     1021 tgacgctttc ccgctgttct tccaagggat gcaggggcag gccttcttcg aggacatcgt
     1081 tcagggcccg gacggcaatg cggacgcagc gcttgaattc atcgaaatca aaggtagcgt
     1141 tcggcgtaaa gggatcgttg acgaagttgg acaggttcat gctgcccaag agacaggagc
     1201 caccggccgg caacggttct tcggcgcagg gattgacgcc ggcaaaggaa aattccggcg
     1261 tgtgggccag gaggttccac gatttgatgc ggtcccagaa cagggcgccc ggttcggcat
     1321 agtcccagtt cgtttctgcg accaggcgga agacgtcggc tgcattgacc aggctttcaa
     1381 tgacctgccc cgtttcctgt cgcttgaaat ggaggcggaa ctgtttgtgt tccttgacgg
     1441 cttccatgaa ttcattgctc aggcggatgg aaatattggc cttagtcacc ttattgaggt
     1501 ccgtcttgag cttgatgaaa tccaggacat caggatgggc acagtcgatg gaaatcatca
     1561 aggccccgcg gcggccgttc tggccgatca gttccgtgac cagggaatag agggtcataa
     1621 aggaaataga accgctcgtt tctttggccg cgttgttgat gcgcgcccct ttcggactca
     1681 agcgggaaat atcgatgccg cagccaccgc cgtagctgta ggtccgggcc aatttcccgg
     1741 cccggtcgaa gatgctttcg atgctgtctt ccggcggcgt catgacgtaa cagttggaca
     1801 aggtaatctt cttgccttca tattccagtc cccggttggc caggatgcgg ccgccaaaga
     1861 gaaatttctt ggttttgatc aaatcggcga cttcttcatc gccgccactg atgcggccga
     1921 cccaatggtc aaaggtttcg ccgttatcgc aatatttatt cttccaaata tccaggccga
     1981 gctgattctc tgcaccgagc cattcttgtt cgttcataat cttcctcctc cactgcgcat
     2041 agcaaacgca tgaccgccgt ctactacatg tagtagacgg cggtcattta ctatactaat
     2101 aatagcagga tggcgggaaa tagtcaagta tcggaaatgc atagtccgac gccttcctag
     2161 tcattgagga catccttttc tatgaaacgg ctgacgacgc gcaggtaatc gctgcggtct
     2221 gtttcaatgc cgacagcatg aggagagtcg gcaaaaatat agagttcttt tttcgggctc
     2281 gtacaattat catagaggct ctttgccgtc ttgaccggca ccagggcatc gtcgctgccg
     2341 tgaaggaaga ggacaggcat cttcatccgc ttcacagcct gcgccggttc cagggaagac
     2401 acggtcttgt gggtatggta gaacatggca gcctggaaga aggggtccag gaggtcataa
     2461 ctccagatag cccgcttatc tcctgaagcc tgccacaatt tctcccgtcc cagggagatg
     2521 atattgccat aagagctatc ggcgacgaca aaggacaggt ccttgccgta ttcactgcct
     2581 gcatacagca gggccatggc cgctccgagg gagacgccgt gcaggccgat gctcatctgt
     2641 tcatcgcggt tgcgcagcca ctggcaccac atggccaggt catcgatttc ccggatgccc
     2701 cattccgtat gggccccttc gctttcgccg tggccccgca ggtcgatgag caggatatta
     2761 tagcccaggc ggcgatagac ttccatataa ggcaggcaca tggaccgatt ctggtagagg
     2821 ccgtggatga ggatgaccgt cttatgggaa tcatgacggt tctcgatata cgtcccgcgc
     2881 aggatcgtcc cgtcctgacc attgacccgg accggttccc agccccgttc cttttcccac
     2941 tggcggacgg tggacacgtc tcggctgtgg ttctcgatgc ccaggagctg gtcccaggga
     3001 gcatcataaa attcttcata agccatgttt ccgatataag ccccgatacc gcccaagatg
     3061 acggccaggc agaccaggaa caggagcagg cgtttcaaga agcgcttcat catgatacct
     3121 tacagcttcc tgtcccggct ggcttctact ttatcctgca tggcagccag aggcgtaccg
     3181 ccaactgctt caatggcggc gatgactgcc ccttcgacga aggggcagtc caggagctgt
     3241 atctgccggt cgtccaggct ttcagcgacc atttctgccg tcatgacggc actgcccata
     3301 tccatgatgc aggcgacgcc atcgccttga tctacggctt cgacagccgc cgtgattttc
     3361 tcgaaactcg ttcccaggct gccgtcttcg agtccgccgg cagcggcaat aggcgcatca
     3421 ggcgccatca tgcgggccag ttcaacggcc ccttcagcgg ccttctggct gtgggaaatc
     3481 aatacgattc ctaccatctt ttccttcact cctaaagatc aggccatttc agccaggacc
     3541 tgcagcaaat agagcgacga cgtagcgccc ggatcctgat ggcccaggct gcgttcgcct
     3601 aaatagctgg cacgcccttt ggtagcgata atggtcttcg tgtattctac gcctttttcc
     3661 gcagcttgag ctgcatcctg caaggcttct ttcaatgatt tgccggccgt tacgtcatcc
     3721 tgcaggactt tcagtgccgg gcacaaggca tcgagcatgg tcttttcgcc ttctgtcgcc
     3781 ttgccgcgca tcttgatgcc gtcgacagcg gcttccatgg ctttgacgaa agcaggtccg
     3841 tcgatttccg tcaggccctt gcaggccatg ccggctttca taaaggccgt cccgtagagc
     3901 gggcccgatg cgccgccaac tgtcgacacg agctgcatgc cgacgccttt gagcagggca
     3961 ccgatgtcac cgcctgccag ggaaggcagc ttcttttcga cggcttcaaa gccgcgggcc
     4021 aggttgatgc cgtggtcgcc gtcaccgatt tcgttgtcga gctcggtcaa atattctttt
     4081 tctgcttcga ttttcttagc catcccttcg acgatggctg ccatctgttt cgtatccaat
     4141 gaactcatcc tctcttattt tttccaggcc agcgtatcgg ccggtgcatc gtagagagcc
     4201 ttcatttcgt catccaggcg caggagagtg atggagaaac cagccatttc gatggatgtc
     4261 atataattgc cgaccatcgt gtcgtagacg ctgatgccct gttccttgag ataatcctgt
     4321 acgaagttgt tgacgatgta cagttccatg agcggcgtcc cgcccatacc gttgatgagg
     4381 acgacgactt catgaccttt ataatcgaga tccgccagaa tctggtcgac gagcatcttg
     4441 gcgatttcat tggccggacg cattttttcc cgatgcgtcc ccggttcgcc atggatgccg
     4501 ataccgattt ccatttcatc atcggccagt tcaaagcccg gttttcccgc agccggaaca
     4561 gtgcacggtt cgatggccat gcccatggag cggacgttgg cgatgacctt ttcggcgacg
     4621 gccttgactt ccggcagttc agcgccctgt ttagctttgg caccggcgat cttatggacg
     4681 aaaatcgtac cggctacgcc gcggcggccc gtcgtataca ggctgtcccg gacggcgacg
     4741 tcatcattga cgacgacata gtcggccttg atatcttcat cggctgccat ttcgatggcc
     4801 atttcaaagt tcatgacatc acctgtatag ttcttgacga cgcagaggac gcctttatcc
     4861 gtcgctacgg ctttgatgcc ttccaggacc tggtccggtg tcggcgacgt gaagaccgtc
     4921 ccggccacgg cgcagtcgag catgcccgtg ccgacgtagc cggcatgagc cggttcatgg
     4981 ccgctgccgc cgccgctgac cagggcgact ttgtcttctt ttttgtcggc ccggacgacg
     5041 atggtcccat aatcgagttt gcgcagcttc ttcggtgccg atttgaccag gccctgaatc
     5101 atttcctctt caacgttgtt gacatcattg acaatttttt tcatgataac cccttctttc
     5161 taggatatag ttaaaagagc ttgtcgcacg tgcgacaagc tcttttaag
//
LOCUS       DAWABZ010000125         5188 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_4075_length_5188_cov_3.483343, whole genome shotgun sequence.
ACCESSION   DAWABZ010000125 DAWABZ010000000
VERSION     DAWABZ010000125.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5188)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4075_length_5188_cov_3.483343"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3730..3803
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1324 a         1321 c         1357 g         1112 t
ORIGIN      
        1 aacaaataat catctaaaaa ataccttagt cacatagtga aaaaagatga ttttatctgc
       61 gcaatgttaa agatgactcc taagtgtaca cggattcttt cagttaccag gttttacaga
      121 aaggggaaaa gtactatggc aaagaaaatc ttcaagacaa tggatggcaa cgaagctgcg
      181 gcttatattg cctacgcctt tacggaactg gcttccatct tcccgataac gccgtcgtcg
      241 ccgatggctg aacacgtcga tgaatgggct gctcatggcc gtaagaactt gtttggcacg
      301 accgtccagg ttcaggaaat gcagtctgaa aaaggggctg ccggtgctat gcacggcgct
      361 ttgaataccg gcgcattgac gacgaccttt acatcttccc agggcatgat gatcatgctc
      421 agcaacatgt tcaagatttc cagcgaactc ctgcccggtg tcttccacgt cgcatcccgt
      481 acagttgcca ccaacggcta tacgattttt gccggccatg acgatgctat ggctatgcgc
      541 ggcacgggca tcagcatgat gtgcgaatcg agtgtccagg aaatcatgga cctggcagct
      601 gtcgtccatg ccacggctat ttcctgccgc atcccgatca tcaacttctt cgatggcttc
      661 cgcacgtccc acgaaatcca gaaaatcgaa gtcctgccgt atgaaaaact ccggcctctc
      721 ctcgatatgg atgccgtccg ggccttccgc aaacgcggca tgaatccgga taatccggaa
      781 gccatttctt tctgcgaacc ggctgaaacg tttatgcagc accgcgaagc cctcaataaa
      841 ttctacgacc gcgttcccga tgtcgctgaa ggttacatgc agaaaatcag tgaaatcacg
      901 ggccgtgaat accacctgtt caactatacc ggcgctcctg atgctgaatt cgtcctcgtt
      961 gccatgggtt ccggtgccca gaccattgaa gaaacggtca agatgctcgt cgataaaggc
     1021 gaaaaggtcg gctgtatcaa cgtccacatg ttccggccct ggtcggaaaa gcatttcctg
     1081 gccgctctgc cgaatacggt caagaagatc gctgtcctgg accgcaccaa ggaaaccggc
     1141 gccaacggcg aaccgctcta tcacagcgta gctgctacct ttgccgtagc agacaatgcg
     1201 ccgaaagtct accacggccg ctatggtatc gcatcgaaag aattcttgcc gggcgacgtc
     1261 gtcgctgcct ttgaaaacct caaggccttc gaaccgaaga acgacttcac tttgagcatc
     1321 gtcgacgatg ttacctataa atctctgccg cgcgtcaaag gcatcacgac aggtggcaaa
     1381 ggcctgaaag cctgcaaatt ctggggcttc ggttccgacg gtacagtcgg cgccaataag
     1441 agtgccatca agatcatcgg cgataacacc gatatgtatg ctcaggctta ttttgcttat
     1501 gactccaaga aatccggcgg cgtcaccgta tcgcatctgc gctttggcga tacgccgatc
     1561 aagatgccgt atctcatcaa taatgccgac ttcatcgcct gccaccgcca gtcctatgtc
     1621 catggcttcg acctcctgcg gggcatcaag aaaggcggta cgttcctgct caatacggtt
     1681 tggaaaccgg aagaactgga tgaaaaactg ccggctaaat taaaacgcgc tattgcccgc
     1741 aacgacgtca aattctacat catcgacgct gtcggcattg ctcagaaaat cggtctcggc
     1801 ggccgcatca acatgatcat gcagtcggcc ttcttcaaac tggccgatgt cattcctctg
     1861 gacctggcta ttgcgaaact gaaagaatcc gtcgtcgcat cgtatggcaa gaaaggccag
     1921 aatatcgtcg acatgaacaa tgaagctatc gaccagggcc tgtctgctat cgtcaaggtc
     1981 gatgtaccgg ccgcctgggc tgatgctgtc gatgaaccgg tcgacatgtc gaaacatacg
     2041 aaattcttcc gcgaattctc cattcctgtc aatgcccttg aaggcgacga cctgccggtc
     2101 agcgcttatg atggcatcga agacggccgt tggccgacgg ggacctgcgc cgaagaaaaa
     2161 cgcggcgtcg ccatgttcgt accgagctgg gatgctgaaa aatgtatcgg ctgtaaccag
     2221 tgttccttcg tctgccccca tgctgctatc cgtccgttcc tgatgacgcc tgatgaagcc
     2281 gctaaggctc cgaaaggcta ccaggacaag aaaatcaaag tggctccggg ctacaagtac
     2341 aatatcgtcg tatccgtcat ggattgcctg ggctgcggca gctgtaccca tgtctgcccg
     2401 aaacaggcat tgaccatgaa acctttcgac ggcgaagaat ataaagcggc tctttgggat
     2461 tacgtcatcg gcctgccggt caaagccaac ccgatggata agaataccgt catcggcagc
     2521 cagttcgaac agccgctcct cgaattcacc ggcgcctgcg caggctgtgc tgaaacgccg
     2581 tattgtaaag tcgtcaccca gctcttcggc gaccgcatga tgattgccaa cgctcacggc
     2641 tgctccagcg tctggggtgg cagtgcaccg gactgtggct ataccaagaa cgctcagggt
     2701 cacggcccgg cttggtcgac atccctcttt gaagacaacg cagaatacgg tttcggtatg
     2761 atgctggctg aaaagacgga acggaaactg ctggaacagt acatcgatgc cgctatggac
     2821 gtcgccagcc cggaactgca ggcagccttt acggaatgga aagaacacaa ggctgaaagc
     2881 gaaggcaccc gcgaacgttc ggataaggtc attgccctcc tggaagctga aaaagacggc
     2941 aaaccggcac tggaacaggt ctatgatatg aaacagttcc tggtcaaacg cagccagtgg
     3001 atcttcggcg gcgacggctg ggcttacgat atcggttatg gtggcttgga ccacgtcttg
     3061 gctcagggcg aagacgtcaa cgtcctggtc ttcgatacgg aagtttactc caatacaggc
     3121 ggccaggctt ccaaagctac gccgacggca gctgttgccc agttcgcagc ttccggtaag
     3181 aagacgcgca agaaggactt gggcatgatg gaagcacagt acggctacat ctacgtcgct
     3241 caggtcgcta tgggcgccga taagaaccag ttcatgaagg ctcttaaaga agccgaagcc
     3301 tacaacggct cgtccctgat catcggttat gcaccgtgca tcaaccacgg cctgaaagtc
     3361 ggccagggtc agagccagct cgaagaaaaa cgggcagtcg aagccggtta ctggcatctg
     3421 tggcgctata atccggaatt gaagaaacag ggcaagaacc ccttcatcct cgattccaaa
     3481 ccaccaacag ccaacttccg cgacttcctc atgggtgaaa cacgtttctc gtccttgcag
     3541 cgcaccttcc cggatacggc agaagcactc tttgaaaaga ccgaagaaga tgctaaggac
     3601 cgctacgaag gctatgtccg catgacaaaa gcctatgatg acaaataaat agttcgttgt
     3661 aagtgaaaaa aggagccgcc ccattaagca agaacacgct taatggggcg gtttttttgc
     3721 actgtagggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnncccgtta ggaatctgga agcttctcct agcagaccgg
     3841 ctagaaaatc acatatatcc tgggacgggc tcgccacgcg cgagccccta cggattatga
     3901 tccccttcgt gagacagcca ctacaagcca cgaactacga gccactagcc atgctcccgt
     3961 ccttctaaga ggccggccag gatggtgatg tagtggcggg cttcttcggg caggggttct
     4021 ccggcaatct tgatccagcc tacggtcatc gtatccaggg gattttcgat ggggatggcc
     4081 tggatatggc ggccgatgat cgacggggct acatggcccg tgccgatggt gtaggcatcg
     4141 gtagaccgta tgacgctgaa cagggtggaa cggtcggtga cgtagatgca ctggcggctg
     4201 gccggattgg gcaggagcag ttcttccgag aactgggact tgtcatggcc ctgttcaaag
     4261 cggacacagg gatattccgc aaattgactg ggaacgacgt gctgacacgc tgccagggga
     4321 tggccgctgc ggacgtaaag acagggcgta aaggcgcaaa gagacgtgaa ttccagcttg
     4381 tttttgtgga ataagtcctg caggaagcgc tcgttcatat gggacaagaa gaggatgccc
     4441 agatggctct cgcgggtgac gacgtcgtgg atgacctgcg acgtccgccc ttcgcgcagg
     4501 gtgaatacgc aggacttgcc cggctggacg tgttgcaggg catggatgaa ggcttcatcg
     4561 gcaaagggat agtgctggga agagatggac aggatctggg gcaggtcctg tcttttttct
     4621 gcctggaaat ggtcgtgcat ggccgctgca tgggagagga tgtgaccggc atagtgcagg
     4681 aattccagcc ctgccggtgt gaacatgatg ccatggcggt tgcgctcgac cagggtcagg
     4741 gagaactctt tttccaggtc ccgtatggcc gtgctcaggc tgggctggga gatgttcaca
     4801 gcctgtgcgg cagccgaaat tgaattgtgc ttagataact ctaagatata actaagttgc
     4861 tgtaaagtca taattaaaca cctcgaatat caaccgtacc tatagaatcg catatcgtac
     4921 ttttattgta tcaaaataaa aaaatagagg aaagttatga gtttttgata caataaagga
     4981 atatttaaaa tgaaaataaa gaataaataa taacgaaaag ctaaaaagag atgtctagcc
     5041 atgacttaca aattataaag aaaatatatc gaaatttgac tttttggaaa ggacatttct
     5101 tcatagaaat gctggaatcg tgagaacaag tcattctcaa tatatgaaaa acctagcaaa
     5161 tgatgaagaa aatttaaagg aaataagt
//
LOCUS       DAWABZ010000126         5183 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_4086_length_5183_cov_2.886895, whole genome shotgun sequence.
ACCESSION   DAWABZ010000126 DAWABZ010000000
VERSION     DAWABZ010000126.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5183)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5183
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4086_length_5183_cov_2.886895"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1173 a         1249 c         1428 g         1333 t
ORIGIN      
        1 tagcaagggc gcccacgtgg ggcgcccgtt cggaataatg gcatatccat catacatgac
       61 aggtgatgca tgaaaacacg cacatatatt agtatacgtt atgctcacat agtcacagcg
      121 ggcttgccac gtgcaggtcc ctacgaaagc ggtccggtgg accgaccgct aacggctaat
      181 ccatcactct tcctgcgtgc gttggaagat ggcggtgact gtcgttcctt tatggagggt
      241 gctgtctaat tccagttcgg cattattggc cttggtgatg tatttggcga tggataggcc
      301 caggccggat ccgcctgtgt ctttggtgcg ggacgaatcg acgcggtaga agcgttcaaa
      361 aacgagttcc aggtcttctt tggggatgcc gatgcctgta tcgctgatga catagcggac
      421 ggtcttttcg tcggcctggc aggtcagggt gacctgtccg ccggcaggcg tatatttcag
      481 ggcattgtcg acgaagatgc gcagcatctg tttgacgctg cccggttcgg ccgtaatcag
      541 ggcttcgtcg ttttgacgca ggatgatctg gtgttctttg tcgatgaggc tgacttcgct
      601 gtatacttcc tgcaggaggt cggccgtatt gattttcgta aagtgtttca acagcgttcc
      661 attattggaa cgggcaaaga agagcaggcg ttcgatgagc tgcttcatat agtccgtttc
      721 tgatttgatg gccgtcaggc tttcgttgag gacggctggg tcgtctttgc cccagcggca
      781 taagaggtcg gcgtagccgg caatgacggt aatcggcgtc cgcaattcgt gggacgcgtc
      841 gctgatgaac tgctgctgtt gtttataacc gtattcgatg cggtccagag cctggttgat
      901 gctgacggcc aggtcgtgga gttcgttctt gttgtcagac agggcgatac ggttgtccag
      961 gcggttgact tcaatggtct tgaccgtgtc attgatgacc tgcaaggggg ccagggagcg
     1021 cttcatgagg tacatacccg tgaagatggt caagatcagg ctgatgagga ccgaagccag
     1081 gagctgtttc gataagagcg acacgaagcc cgtttcttta tcggggcgct gagaaaagcg
     1141 caggtagtag ttctttccct gtttgtccgt ccagagctga taataggaga attcagacga
     1201 ttcgttgcct tggacgctgg tgggcagcgg ctttttctgc ggattgctta tggcgtcgtc
     1261 gatgtagcgg tcgctcagtt tgcggacggc atgggtcggg ccgttgtcga cgatgagatg
     1321 gcctgcgccg tcataaattt gtaagttcat cgacggtggt acgttggccc ggacaaatat
     1381 cgtcgtatcg acctttccgt agtcgtccag gtattggatg gccgccgcag cgcgggactt
     1441 caattcatcg cgcagggagt cgttcatgat gtagtggacg ctggtaatgg ttacgaccga
     1501 tacggtcagg aggatgaaaa agacgataga cgaatagagc aggaccattt tagcgaaaat
     1561 gggcagggga gcgatgagca ggtgctgtgt cagccaatga ttaatcttct gccacataac
     1621 cgactccacg gactgtacgg atgtagttgc gcccgttcgg gcccagtttg ctgcgcaggt
     1681 aacggatata gacgtcgacg atattcgtgt cgccgatgta atcgtagccc catacctctt
     1741 cgacgatgcg cgaccgcgag aggacgatgt tcttgttccg gactaaatag gcgaggagct
     1801 cgaattcttt cttcgtcaat tttaattttt tcgttccctt catgacttcg tgacggtcga
     1861 ggaataaagt gatgtcattc agctggagaa aatttccccg gtcggccagc tgatggcgtt
     1921 ctcgcaggag gacacggatg cgggcaaaga gttctttgct gttgaagggt ttggtcagat
     1981 agtcgttggc gccgatgtcc aggctgacga ctttcgtttc gatatcgtct ttggctgaga
     2041 gcatcatgat cgggacgttc gatagctccc gcgtccgctt acagatggtc aggccgtcga
     2101 cgtcaggcag catgatgtcc agcaggatga ggtcgaagtg ttcctggccg atgcggtcca
     2161 gggcagcggc gccgttgtat tcgatgccac attcatagcc ttcatgttcc aactccattt
     2221 gcaggaagcg ggcgatttgt ttttcgtctt cgacgattaa gatatgtgtc ttttcggtca
     2281 tataacaacc tccccaaaaa agaatttgca cactctaagc catatacata ctattataac
     2341 gtataaattc ccttttttgt actactcatt cggacattgt tacaaaaata tcacgaaaat
     2401 ttaatctatt tttaatcttt agggatttac ttttcgccag aacctgttat tataatatat
     2461 gtagaacaga tcatctacga gacgttggag aagtaagttg ttcatatacc cctcgaacac
     2521 ccctatctta ttttcttcaa ctgctgagat attgtcttcc cccgatgata tcttgcaaaa
     2581 agagaaagcc ggctcccctc gccggctttt ttctatttct acggaaaatg attttggcga
     2641 aattttgaga aaattttttt ggacaatcgc aaaaacctag gaaataccta tatctttacc
     2701 cattcggtat tcattgacaa gtctgtctat tcgtgtaata atgaggaaga cgaaggggag
     2761 attcccccga aggcttgatg tttggaggtg aaaaccatgc atgttatttc cgacgaatgc
     2821 gtaaaatgcg gcgcttgcgc tagcacttgc ccgaccggtg ccatcgaaga aggcgaaacg
     2881 aaatacgttg taactgattc ctgcatcgac tgcggtgctt gcgaatccgc ttgtcctaca
     2941 ggcgctattg cagctgaata attgcttcgg caaaaggctg tcgcagaagg ctttggccat
     3001 catgcggcgg ccttttttta ttgaattttc cctgtttctg tgataggata aaagcactgt
     3061 aagatgcata ataggaggag tttgctttgt atgtattagg cattgccctg gccgtgaccg
     3121 ttgtcgatca gcttacgaaa tacgctatcc atcaggccat ggtccttcat gaatccattc
     3181 cggtcattcc gggatttttt catattactt atattttgaa tcgcggtgct gcttttggga
     3241 ttttagaaaa tcagcagtgg ctctttttag tcatggcctt ggccttattc ctgctgtatg
     3301 gtctattccg caagaaattg ccggcgacgc cctgggtcta tggcggggcg gggctgcttt
     3361 tgggcggtgc cttgggcaat gccctggatc ggacgctccg cggggcagta acagatttct
     3421 tcgattttcg catctggccc gtattcaacg ttgccgatat cggtatcgtc gtcggcgtcg
     3481 cacttttgtt atggtatagt tggacccata tttcggataa atgaggatga agacatggaa
     3541 gaaaagaaat taatagtcac agaacaggaa cagggaaagc ggctggacgt ctatttgacg
     3601 acggccctgg atatttcgcg cagttatgcc cagcagctca ttcagcgcaa ggaagtcact
     3661 gtcaacggta aggaagccaa ggctaaccgc cgcctcaacg aaggcgatca ggtacaggct
     3721 gtcatcgaac tccaggaaga atataaggtg gtcccgcagg acatcccctt ggatatcgtc
     3781 tatgaagata aggacatcat cgtcgtcaat aaggcccgcg gcatggtcgt ccacccggct
     3841 gcaggcaatc ccgacgggac cctggtcaat gctctgctct atcactgcca gggcgagttg
     3901 tcgggcatca acggcgtcat ccgcccgggt atcgtccatc gcctggacaa ggatacgtca
     3961 ggcgtcatgg ttgccgccaa gaccgatgaa gctcataagg ggctggccga acagattaaa
     4021 gaacattcgg cgcaccggac gtactgggcc ctggtccacg gcaatatttc ggaagaacgg
     4081 ggcgtcgtcg atgcccctat cggccgccat cccaaagacc gggtcaagat ggccgtcacc
     4141 tttaagggtg gccgcgaagc tgtgacccat ttcaaagtat tgaagcgcta tggcgattac
     4201 acctgggtcg aatgtaagct ggaaacgggc cggactcatc agatccgcgt ccacatggct
     4261 tatatccacc acccggtcgt caacgaccct ttatatgggt ataagaagga cgatttccct
     4321 atcgacggtc aggcccttca ctcgcatagt ctcgatctgg tccatccgat tacggggaag
     4381 gccatgcatt ttgaagcgcc agcgccggct gatttcctgg cctgcctgga acgggcggaa
     4441 aagaaagact gacaggtggt gagtcacatg tggaaagaaa agacactgct catggacgaa
     4501 aaagcaatcg gccgggctat ccgccgcatt gcccatgaaa tcattgaacg caataaaggc
     4561 ctggacaaga cggtactcgt cggtatccat acgcgcggcg tccccctggc tcagcgcctg
     4621 ggccgggaac tggcagctat cgaaggccgg gatgtgcccg tgtatgatct cgatatttcg
     4681 gcttatcgcg acgacgaccg caagagcccc atcgccgtca gcgatgccgg caaattcgat
     4741 gccgacggcc agacggtcat cttagtcgac gacgtcctgt tcacgggacg gacggtccgg
     4801 tcagccctca atgccatcat ggaactgggg cggcctcagt tcatcgaact ggctgtcctc
     4861 gtcgaccggg gtcaccggga actgccgatc cgtgccgatt atgtcggcaa aaatgtgccg
     4921 acgtcgcgga aggaagacgt gtcggttcaa ttaaaggaaa ccgattcgtg cgaccgcgtc
     4981 gtcttgcggg aacagaattg attttcccct tatttcttgc tatacttaag tgaacatcgc
     5041 acgaagtttt cgaccatctt atctggttat cctggcgtta atgtttgaag attgatgtaa
     5101 atttttaacc atccgctaac aactgactgc tagccactaa ccacttaaaa gatcttgtcg
     5161 cacgtgcgac aagctctttt aac
//
LOCUS       DAWABZ010000127         5061 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_4200_length_5061_cov_2.740911, whole genome shotgun sequence.
ACCESSION   DAWABZ010000127 DAWABZ010000000
VERSION     DAWABZ010000127.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4200_length_5061_cov_2.740911"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    254..263
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1077..1086
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2015..2024
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1219 a         1320 c         1364 g         1128 t
ORIGIN      
        1 cgtatgtcgt catcaaccta ctcacaggca aacgggaaaa gatttccctc ctcatgtact
       61 gcctggccgt catcttcgtc atgaagtaca tcttcttgta agagatggag ttaacagtta
      121 gcaactagtg gctagtgatt cgccaaaaat gcgtaggggc gccacgtggg gcgcccgctc
      181 ctgaaaaatg gttccaacgc tttattatgt cggaaaaatc gaacatatcc tgaaacgggc
      241 tcgccacgtg cgannnnnnn nnngcttttt ttgttatacg aaccacgagc cactaacctc
      301 acttcaacaa gcctttttcg gcgacgaagt cggagaccat gtcggcggca gtggccgtaa
      361 tcttgatgca gtgcttacgg tattccggcg ttccgaaact gagggtgttg agggcccggc
      421 agcaggtggc gccgaagtgt tctttgaagc ggtcatggaa ctcgctggtg tatttgtaga
      481 cttcgggcaa gggcttttca tcgggtatga gccggccgac gagggcgccg atgaccagac
      541 aggcaccgct caaggcccca cagacacagc ccgaccggcc gatgccgccg cccatgccgg
      601 aagccatgcg gatagcgacg tccggcagct ggagattcat cgtgtcgtta caggtcttga
      661 ggaccgattc aatgcagtta ttgccctgca aatggtattg ttctgctaat ttatattctt
      721 tttccatgat actttatcct ttctgaaatt aactgcggcc ttcgccgtct ttgaggtcgt
      781 cggcgatctt gtggaggact ttcttggcct gctggcggcg atgccagtac cacaggccgc
      841 cgtagacgat ggcgatgacg atgaggacga tagtcaatcg catggaatgt tcttccatca
      901 tgaccaggtc gtgaccaacc aggacctcac aggcgacaga cggcaatttg ccgatgaaac
      961 agcccaggat ataatcgcgg tagctgatgc ggctcagcgc ccccagggcc gtgaccaggc
     1021 cgttcggcga atacgggacg gcccgggcca gggtcatggc ctgccaggta ctgtaannnn
     1081 nnnnnncatc ttgctctttt caatgacctt cttggccttg ttgcggaaca aagtccgcat
     1141 gaagaaaaag ctgatgatga cgccgattgt ttcggctacc caggaaacga cagtcccacc
     1201 ggcaacgcca aaaaggacgc cattggccgt ggaaatgaag atagacggca aaaagccgac
     1261 gatattgatg acgatgtcga tgatcatgct gaccaggacg gcccagacgc cgaaagagcg
     1321 caggtagtcg atagtcccct gcatattgcc gctcgtcgac agctgccaga ccgtgctgta
     1381 aaagtccggg atggccacat agatagcggc cagcatgatg agcagcatgg caaaaaaggc
     1441 gattttaatg tgtgtttcat gaatctgttt cgtagtcatg ttatgcctct ttctgcttcc
     1501 gttcctgcat cttggccatt tcagcaatct tatcgtgctt gagcttggtc aggtacatgg
     1561 aaccgccgat aatgagtaca ccgccggcga cggtcaagcc ggtgaggatt tcgccaaaga
     1621 tgacgaagcc ccaaagggcg ttgaagaaga cgcctatata agaaacaaag ctgacgacaa
     1681 ccgggttggc cgttgcatag gcattggtca tccaaacctg ggcgatgacg ctgaacaggg
     1741 ccaggccgcc gtaataaatc cagtcgccgt gagccggcat gacaaagcca tcagtcaggg
     1801 ccatgaggac gatgcccgac agagctgcac agaccaaaaa gtacaggacc acttcataag
     1861 gatggtggcg tccagacccg gtgagcttgc tgactgtcgt ataggcaccg gccgcaaaga
     1921 cggcctgccc cagggcgaac agggaataga cgttgaatga cgaaaagttc cagggccgga
     1981 cgacgaggca gccgccgatg aagatgacca ggagnnnnnn nnnngggcag cttcgaccca
     2041 ggcggcagga cttctttgag gaaaatcgcc gcgaagagca tgacgaagaa cgccgacaac
     2101 tgggaaatga tggatacatc ggccaggggc atcccgcgca gggcatagaa aatgcagatc
     2161 atgccgatac cgccgaggac gccgcgcaag gtcagcgtcg gtatatcccg cttcgacaga
     2221 tgtacgtgtt ggatgcgcat gagccctaat atcagaaggg cactgaggaa accgcggaag
     2281 aacgcgattt ccccagtgct cgtcgtatag gataattctt ttacgatgac attctggata
     2341 ctgaaggcca aagccgataa gattgcgtaa atgacacctc tactcaaatc aaagcctctt
     2401 tcctagtgct tggccgcaat ggcgtcgacg aaggataaga cagctttctt catgtcttcc
     2461 ttgtcgatgg agtcgctgaa gcggatgggc ttatccttga ggtccgccaa aggcttgggc
     2521 agggcgatgc ccgtgacgct gctgatgtcg gccagcatgt cgaagggatc atcgctctga
     2581 cgcagggcca gggcttcgca gatgaccggc gggaacttgt agggatgagc cgttgccatg
     2641 atgaccgtgt ggcggccttc gtcgaggccc tggcgatggc ggttcatgcc gacgccgtaa
     2701 gcgacggccg tgtgcgggtc gaggagatag ccgaaatcat tgtagacgtg gttgatgacg
     2761 gctttcgttt catcgtcgtc gatccagccg ccttcaaatt cctggcgcag cgtcttcaat
     2821 tcgtcagccg atacgctcat cttccccgtt tccaggaggt ccttttccca ctgggccact
     2881 ttggcggcat cttctttggc gacgtagaac aagaagcgtt caaaattgct cgacaagagg
     2941 atgtccatgg acgggctcga cgtcgtgtag aagtcgcggt tcatgtcata gacgccggtt
     3001 tcaaagaagt cggccaggac cttgttctgg ttggatgcgc aaatcaactt gccgataggc
     3061 acgcccatct tcttggcaaa atagccagcc aggatattgc cgaagttccc cgtcgggacg
     3121 atgacatcga acgtttcgcc ttcgctgatg gcgccgttat cgacgaggga gacatacgtc
     3181 gatacgtagt aggcgacctg cgggaacaag cggccgatgt tgatggaatt ggcgctggag
     3241 aagaggacgc ccttgtcgtt ggcataggcg ttgacttcag cactggtaaa gacgcgtttc
     3301 aagaagctct gggcgtcatc gaaattgcca taaatggccg ataccttgac gttattccct
     3361 tcctgcttct gcatctgctg cttctgcatg gggctgacgc cttcgctggg ataaaagacc
     3421 atgatattcg taccaggtac gtccttgaag ccttccaggg cggccttgcc cgtgtcgccg
     3481 ctggtcgccg tcaggatgag gatatcccgg tcctcttttt cctgcttctt ggcagccgtc
     3541 agtaaatacg ggaacaagga caaagccagg tccttgaagg ccaacgtccg gccgtggaac
     3601 aattcggcaa tggacaaatc cgtatcgatg gaatggagcg gtacgatgcg gtcatcgcgg
     3661 aaggtcatat cattataagc cgaagcaatc atctgcttcc gttcttctgc tgtgaaattc
     3721 gtaaagaaat gggacaatac ggcttcagca atctcctggt aagacttgcc agccagttct
     3781 ttataggaca ggaccttttc cgggaacgat tccggcacat agagccctcc gtccgcagcc
     3841 agtccatgca ggagcgcata agacggcgaa acacattcgt cagatcgtgt acttctaaaa
     3901 ttcatcgatg aaatccccct tttcagctaa cctcatagaa tagaatagct ataagtattt
     3961 atattataaa gggaaatagc cacattgtcc actattctgt gaattggcct aacggccccg
     4021 cagttcgcag ttcgcaggcc gcctcggact caaaatgccg ctttgcggca acaaactcag
     4081 aatgacgaca tctcctcgag tgaacctcag ctcgtaaaaa aaggccccaa gtggggcctt
     4141 cgtaggggac gtcctgaaca aggcgtccag cctctgctgt ccgttttggc aaccgtcttg
     4201 cgccttccgc tggtcggcac ctgacaggcc aaaaaggcca cgtgaatcta ggctttgaca
     4261 gccacccctt ccagatgcag gagggtgatt tttttatcga tgccgcctgc atagcctgtg
     4321 gctctgccat tggcacctaa gacgcgatgg cagggaatga tgatggaaat ggggttatgc
     4381 cctacggcgc cgccgatggc ctgagccgac atcttctttc ccgtccgttc ttcaatctgt
     4441 ttagccagat caccataggt cgtcgtctga ccatagggaa tctggcagag cagataccag
     4501 acgaggcggc ggaacgcact gccctgcggc gccaggggca gcgtcatgat agccggcatc
     4561 cgcccggcaa aataatcatc cagccagtgc gccgccttgc gcaggatagg cgtatcctct
     4621 tcacgcagga cttccccctg caaggcacca taacaatact tctgcccttc aaaccaggtc
     4681 ccgaccagcg ccgtatcact agccgccaaa atcatcggac ccaacggcga ttcatacatc
     4741 attttgtagt acatctgatt caccctctct ctacttctga aagtaatatc tctttagatt
     4801 gcaataattc aggatggcga accatatagc cagttaaatt tttccaagct tcattctctg
     4861 cgatataggg aataggtcct tcacaaattt cattttcggc gtgattttcc cccaatggtt
     4921 ggtaaaaatt attttcaaaa gtcccataag catctggacg aagccaatgg tatggagaaa
     4981 ctaagtggtc aataggttca gttaaagaag aaaataacga ataatacgta aatcccttag
     5041 gggctattaa ttcaaaaatg g
//
LOCUS       DAWABZ010000128         5011 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_4248_length_5011_cov_2.567595, whole genome shotgun sequence.
ACCESSION   DAWABZ010000128 DAWABZ010000000
VERSION     DAWABZ010000128.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4248_length_5011_cov_2.567595"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1079 a         1390 c         1395 g         1147 t
ORIGIN      
        1 acattgtcta ctagacgttg ccctataggc agggttataa tggagaagat agaaaggatg
       61 attagatatg accaaattgc ggcctagaga tatattcttc aacgaccctt attttgatta
      121 tgcaacctgc agcaagcccg gcgacctgcg gctggccaag cgggtagaag tattcggcaa
      181 atggtacaag accccgagcc tggtgacgac ccttctttcc caggaacggc tgaccttcag
      241 ccgccacacc ggcacagacc aggaagacgc cttggtactg cgcatctacg aacggacggc
      301 cgaccggctg ctcatcaata cgtattatac gtccaaacag aacccggaag aaatctacgc
      361 cggcacgatc cacctcgaac gctacgatat ggcgcagact atggaatctt tttcctatat
      421 gggcatcatc gacctgctca tgctccagac ggacagtcag attctcggct tgaccgacgt
      481 ctttgaagac gtgcagatgg ccaagcacgc ggcagcccat gccctgggca tctatctcat
      541 ggtcatctgc gaccagccct ggtccgacga agccgtcact ggcggccagg aaacgccggc
      601 catgatgatg ggcaaagaaa aagaagaact tcagcagctg cgggaagaaa aagcccagat
      661 ggacgccgac cgggaagaac tcctgcgcct gcgcctggaa gccaagaacc gcacggccgt
      721 cctttctgaa ctggaacggc tgcgccagga actcgatgcg gaaaggacgg gccgcgaaca
      781 ggaccgcaaa cagtatgaag accgtatccg caagcaggaa cagtatatcg aaaagataaa
      841 ggaaacggca tccagccaga ttgccgaact cctggccctg cgcaaggaac tcaacaagcg
      901 ccaggtgcgg ccggatatga ccaaaggcca ggcgccgcgg aaagtgacgc cccaggaagc
      961 gtcgcgttcc gtccgggacc cggaagaccg cggcctggac ctgccctcct tcatgaccca
     1021 gcgcgattat acgcgccagc tggaccgctt cctcacgggc cgtcagatag ccctggccgg
     1081 cggcgatccc gactggcagc gccgcatcca gcgccgtttc ggtgccgtcc tcctcatcag
     1141 cggccagccg ggcgatgacc ggatgctgaa agaagcgtcc ctcttagtcg tcaacctgga
     1201 agacggcaag acgcccctcg tccgctgggc cattacggaa gccaaagaaa ataactgccc
     1261 catcctctcc gtcggcccga ccaacctcaa cggcttcgcc aaagccatcg tagggcgggt
     1321 tagtggttag tggctcgtgg ttcgtgatta aaaaaacata ctattaagcc cgaatgggct
     1381 atgatcgaat atcacagcgg gacgccttcc aggcgtcccc tacgcacaag gccacgggcc
     1441 gtgtattttt ttgtgatcgg cccatagttt gtaaagatac ttttaaagtt aaattcattt
     1501 gctaactgct aacagctagc tgctgactgc taaaaaggac tgtcgcacat gcgacagtcc
     1561 tttttctgca aactccctgc tatcaactat tctttttcgc cataatggca gccgcgcggg
     1621 ctacgatgat ggcagcgagt tcgtctttat ccattttttc taatttttcc atagagccgt
     1681 ccgggaaaag cagggtggcg atgttggtcg tccctttgaa gccggctcct tcggcactga
     1741 cgtcgttggc gacgagcatg tcgaggttct tcttcttgag cttcttcgtg ccgtattcga
     1801 tgacattcgt cgtttcggca gcaaagccga ccagggtctg atgggtcttg cgctggccca
     1861 ggccatagag gatatccggg ttcttggcca tttcgatggt cagggtatcg tcgtttttct
     1921 tgatcttgtt ttccgccgga tgggccgaac ggtaatcggc gacagctgcg gctttgatga
     1981 ccaggtcgca gtcgtcataa taggaatcga cggcagcttt catgtcacgc gtactgccga
     2041 catcaatgcg gcgggccagg cctacaggtg tcttgaggtt atccgtcgtc ccggcgacga
     2101 gggtgacttc agcaccggcc atgacggcag ccttggcgat ggcaaagccc atgcggccgc
     2161 tggaatgatt gccgatgaag cggaccgggt cgataggttc ccgcgtgccg ccagccgtga
     2221 cgatgacctt cttccctttc aggaggtcat tgcggcaggc caggagctcg atgtagtcga
     2281 cgatggtatc cggttcgggc aggcggccga cgccgtcgat gccgcaggcc agatgaccgc
     2341 tgtcgggagc gatgaaatga tagccgaatt tcttcagctt ggccatattg tcctgagtaa
     2401 tggggttgag atacatgttg gtattcatag ccggtgcgat gaggaccggt gccgtcgtag
     2461 ccatgaccgt cgtcgacagc atgtcgtcgg caatgccgtt ggcgatcttg ccaatgatgt
     2521 cggccgtcgc cggtgcgatg acgaagacgt cggcccagcg ggccagggcg atgtgttcta
     2581 cgttgaattc aggcacatcg tcgaacatgg atacggcaac gggattgccg gaaatttccc
     2641 gcatcgtcaa gggcgtaatc agtttcgtcg catggtcggt catgatgcat ttgacttcgg
     2701 ccccccgctt gcggagctga ctggcaatag aagctgcctt gaaggccgca atgccgccag
     2761 tgattcctaa gacaatcttt ttcttttcca agctcatgga aaacacctcc gtgttattta
     2821 atgccgtcac gcggtgcttc atacgtgatc ttgccctggt cgatttcttc catggcaatg
     2881 gaaactactt tcgtcgtatc gacgtcgacg aggggttcgt cgccatcggt aagctggcgg
     2941 gcccgtttgg cagccagcgt aaccagcgta tatttgctgt ccactttagt catcagggat
     3001 tcaagagtcg gtttaattaa catagttcat ctctcctatt atttgtacaa ttctttcgta
     3061 tgggcgatgc gatgggcatt gcgcgtcact ttacactgtt cggctttcaa gatagaagcc
     3121 gcttcgtcga cggccttatc caaatcgtcg ttgacgatga cataatcata gcggtcgatc
     3181 caatccagtt cttcccgggc cttggccagg cggccggcga tgacgtcgtc cgaatcggtc
     3241 tggcggccgc ggaggcgttt ttccaacaca gcccggctgg gtggtacgat gtagatgaag
     3301 acgccctggg gataccgttc tttgacctgc atggctccct ggatgtcgat ttccagcatg
     3361 acatgcttgc cttcttccaa catctggtag acgtacttct tgggcgtgcc gtagtaatgg
     3421 tcgtagacct tggcgtgttc caggaaggca tcttctgcca ggagctgttc gaaccgcgcg
     3481 ttatcggcaa aataataatt gacgccgtct acttccccgg gccgggggtc acgggtcgtc
     3541 atagaaatgg aatactggat attggggaac tgttcccgca gagcagaaca gatagtcccc
     3601 ttgccggctc cggacgggcc ggacacgacg atgagcaatc cggaatcatt catagcatta
     3661 ctactccttt tctgtttccc cgtcgtcagc ggcatcttcc cagaaggaat cgctgccggc
     3721 gggaacgatg cggtgagaaa tcgtctcggg ctgcaaggcc gacaagatga tccagccgtt
     3781 gtccatgacc aggaccgacc gggtcttgcg gccaaaagtc gcatcgatga gggtattccc
     3841 atcacgggca tcctggacga ggcgcttgat gggcgccgac tcaggactga tgatggccac
     3901 gactttgtcg cccatgacga cattgccgaa gccgatattt aataagttga aattcatcat
     3961 tcgatattct gcacctgttc gcgtatcttt tcgatttcat tcttgagctg caggaccgct
     4021 tcggtcacgg ccaggtcgcc ggccttggag ccgatggtgt tggtctcgcg gttcatttcc
     4081 tggatgagga aatcgagctt gcgccctact tcgccgggac tttgcagcat ggcgtgcagc
     4141 tggttcaagt gactgcggaa gcgaacgact tcttcggtga aatcgacttt atccgaatag
     4201 atggccactt cctgcaagag ccggtcttca tcgggaacgg cggcctggac cttatccatg
     4261 agggccagga ttttcttttc cagccgttct tcataggcct ggacgatgat ttccttgcgg
     4321 ctggcaatgg attcgacgag ggctgccatc ttatcggccc ggctgaggag atcccggctg
     4381 atattggccc cttcccgttc gcgcatggcg accatgctgt ccaaggcccc ctgcagggcg
     4441 gcttcaaaag gcggccagct ggcatcggca tcgatggccg gcggcgtctg gacgaaccag
     4501 tccttggtca gggacgtcac atcgccgagg gtcacggcct tgccttcaaa gaaacggtcg
     4561 ttcacgtcct gcaaggccgt cttgacggcg cctaaggcag cgtggtcgac gcggacatcc
     4621 ggtgccgccg ggtccaggtc ctgaacggtg acgaagacgt cgactttgcc ccgcttgagg
     4681 atggccttga tgccattgcg caaccggtct tccagggcca gataagcatg aggcatgcgg
     4741 atattcaatt ccaaaaaacg ttggttcaca gcgcgcattt caagagtgac agccagtttc
     4801 ccgtcgttgc cggaaccgct gccaaaacca gtcatacttc gcatttacga acccccttat
     4861 tagtctattc aatatctcat tataccctaa acggcttaat ttttgcaata ttttaccaca
     4921 aattaagcaa ttgaacctct cccgcttata gaagcaggag attcttgaga aggttgctgt
     4981 ttaagtttcc tcctgaaatc aggatagcct t
//
LOCUS       DAWABZ010000129         4967 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_4284_length_4967_cov_2.725366, whole genome shotgun sequence.
ACCESSION   DAWABZ010000129 DAWABZ010000000
VERSION     DAWABZ010000129.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4967)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4967)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4967
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4284_length_4967_cov_2.725366"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1115 a         1359 c         1443 g         1050 t
ORIGIN      
        1 cccctacaaa ctgcaaaata acatcggtct gcaatcaatg agccgtttcg gcagcaggca
       61 gttcgctagt gcagtgcggg cagcgggttg catcgtccgc gatgggctgg cggcagaacg
      121 ggcagagccg cggttctgga gccggttccg gtttgggcag gaagcggttc agctgtttga
      181 ggaccatgaa gatggcaaag gcgataatca ggaactcaat gaccgtgttg agaaaggaac
      241 cgtacgcaat gacagggatc ccggcggctt tcgcttcggc cagggaatcg acgtgttctg
      301 tgctcaaagg gatgaagaag tccgaaaaat caatcccgcc gaggaggacc ccaaatgggg
      361 gcataataat gttgttgacc agtgacgtga cgattttccc aaaagctccg ccgacgacga
      421 caccgactgc catgtccagc acgttcccac gcatggcaaa ttctttgaat tccttgatga
      481 tgcccatgtt ttccactcct atctgtggtg taaaaaataa attataaaaa aatagggact
      541 cgcatgatgg cagaaaccgt cgtgcgagtc cctattcatc atagcggaaa aagggcaagc
      601 tgacaatgat tttttgtttt tgttatattt atttaataaa gcgacaggag ctgcggcaac
      661 aggcctgatt ccttcgatac attccaggat gccgacggcc aggacgaacc agaggacgtc
      721 gccggcagcg tggcggaaaa ctttcggttt gactgtatat tccttaggtt catggtagag
      781 cgaccatttc gggaaaaacc gaggcacttc ccggatgtag gcgctgtacg gttccccgaa
      841 gaggcggtgc agccgggctt cttccgtatg gatgaccggg atgtacatga cggcgaagag
      901 gaggaccagc agcaccgtca gggtcagcga ttccgtgcac agaccgacgc cgatgccgcc
      961 gaggaagctg aagaaataca acggattccg gcagaccgag tacggccctt cgcggaccag
     1021 gacgtcattc ttatagccgg caatatactg ggcgcaccac atgcggcctg ccatggcggc
     1081 gccgacgcag agacagccca cgaggaataa gatgcccgag atatacgggg ccgtgacatc
     1141 caattccttt tgcgagaaca tgaataaaaa taaaaacaga agcccaatga cttgggaaat
     1201 acgggtgcgg taatggtcca gaaaatacat ggtgactcct cctcttgtcc taattgataa
     1261 ggccagtata acggtcattt ctaacgaaag attaggcaaa agttaaagga attataaaga
     1321 tttgtgaaca gactgcgtat tctttgtaac aacttttatg atacaataaa agaaacgtca
     1381 ttcgtccatt tttaaggagg catctgccat gttcaaaaaa ttactcatat ttaccctcag
     1441 cgccctgctc tgctgcggcg cggctgtcgc ggccgacacg acgaccatga ccgacaaggc
     1501 ccgccaggct gaaacacagt ccctgccgtc gacgatcaaa catctcgacc tggtcctggt
     1561 cctcgataag agcggctcca tgtatggcct ggaaagcgat actatcggcg gctacaacgc
     1621 catgattgcc aaagaaaaag acctcgacgt cgacaccaag gtcacgacgg tcctgttcaa
     1681 caataaagtc gacatcctga cggaccgcag caacattaac agcctgtcgg acatgacaac
     1741 caaggattac cgcgtcggcg gctcgacggc tctcctcgat gctgtaggca acacgattgc
     1801 caagatttcc gacacgccgg gtatttctga caaagatcat aaggtcatct tcgtcatcat
     1861 cacggacgga caggaaaatg ccagcacgga atattccaag gcgacgatcc agaaaatgat
     1921 ttccgataaa aaagcccagg gctgggactt cattttcctc ggagccaata tcgacgccgc
     1981 ttcggaagcc cagaacctgg gtatcgatgc cgaccatgcc gtcaagtacc gcaatacatc
     2041 gacgggcgtc cagaagaact tcgccgccat tgccgcttat acgcgcagtg ccgtccagca
     2101 gcaagacgac gactcctgga aagataacat cgaaaaggat aagtaaggct tatttcaaac
     2161 ctgccaattt ggccgggtcc atggctttgt cgaactgctc tgccgtgacc catcccgtct
     2221 tcagggctga ctctttcagg gtcagccctt ctttggctgc ctgtttggca atggtgcagg
     2281 ccttgtcata gccgacgagg ggcaccaggc ccgtgaccag gatgagcgag ttgtagaggt
     2341 tggtcgcaat ccgttcgcgg ttcggttcga tgccgacgac gcagtgcttg cggaaggaat
     2401 ccatgcagtc cgtgagcagg cggatagact ggatcaggtt gtaggcgatg acgggcatga
     2461 agacattgag ctcgaagttc ccctggctgg cggcgatgcc caaggtcgcg tcgttgccca
     2521 tgacctggac ggcgaccata gtgacggctt cgcactgcgt cgggttgact ttgctgggca
     2581 tgatggagct gcccggttca ttttcgggga tacggatttc gccgatgccg cagcgcggtc
     2641 ccgacgacag ccagcggacg tcattggcga tcttcatgag gtcacaggcc agggccttga
     2701 gcatgccgtg gacgacgacg agttcatcct tactggtcag ggaatggaac ttgttcggcg
     2761 ccgtatggaa ggtcgtgccc gtcagctggc tgacgatttc ggcaaagcgc acatcgtagc
     2821 ctttcgggca gttgatgccc gtgccgacgg ccgtgccgcc catggccagg tcgcggagga
     2881 aatcgacgcc ctggaccagc atatcccggt cgcgctgaac catgcgggcc cagccgctga
     2941 attcctggcc cagcgtcaac ggtgtggcat cctgcaagtg ggtccggccg atcttgacga
     3001 tgtccttgaa ggcttcctgt ttggcccgga gcgcctgata gagggcttcc atggcgggga
     3061 acaatttttc ttcgatgagc aggacgccga ccatatgcag tcccgtcggg aaggtatcgt
     3121 tcgagctctg ggaatgattg acgtggtcat tgggatggac gttcaagtcc ttgccctggg
     3181 cctgtaaaat ctgcttggcc cggttggcga cgacttcgtt gacattcatg ttgaactgcg
     3241 tgccgctgcc ggtcatccag acggccagag ggaaattgcc gtcgagcttg ccggccagga
     3301 tgtcatcgca ggcctggacg atggcgtcgg cccggtcgcc gtcgacgacg cccaattcct
     3361 tgttggtcaa ggcagcagcc ttcttcaaat aggcaaagac cgtgaccatt tcaggcggaa
     3421 ttttttccgt gccgatctta aaattctcga aactgcgctg tgtctgggca ccccagagtt
     3481 tatcagccgg gactttgatt tctcccatcg aatctctttc tatacggtat tccatgaaaa
     3541 cctccaatca gattatataa aacatagcgg actcaggcgg gcgccctttg ggtcgcccct
     3601 acgttgggtc cccacagggg accattcgag gaacgatgaa cgacctacga caagcccatg
     3661 ggattgggct ggaagatgcg gccgtccatg agttgcaggt catcggcgat gagcgggcgg
     3721 aattccatct ggctcaggat gtcccgttca aggtcgacgc ccggggcgat ttctgtcagc
     3781 atgagtccct gcggcgtcag gcggaagacg gcccgttctg taacgaccag gacgtgctgg
     3841 ccgtgggctg cggcgtagtc cccggaaaag gtgacctgtt cgatatccgt cttgaatttc
     3901 tggtacttcc cttcccggtc gatgaccagc ctcccgcctt cacagtgttc tcgcagaccg
     3961 ccggctgtaa aggtccccat gaggatgagt ttcttcgtgt gctgggccaa gtcgatgaag
     4021 cccccgggcc cggtgacgcg gccgtggaat tttgagacat tgacattgcc catggcgtcg
     4081 atttccgcca ggcccagggc acagaggtcg aggccgccgc cgtggcaaat atcgaacatg
     4141 tcggccatct tcagttccgc ttcggcattg acagcggccc ccatatcgag gcccgacaag
     4201 gggatgccgc cgatgatgcc cgaatcggcc gccagagtca ggctcggccc gaagccctct
     4261 tcagcggcga cagagccgat gatttccggg atgccgatgc ccaggttcat gacgtcgcct
     4321 tttttcagtt ccatcgccgc ccggcgagca cagattttgc gattgtccag aggatgcggc
     4381 ggcagggact ggatggactt gcgcccttcg ccgctcagtt ccggccggta gccgtgggtc
     4441 gaaaagctct ggacgtggta cttcggccgc ggcacgacga cgtaatcgac gaggaaatgg
     4501 tggatcttca cgagccgcga atcgaggctg ccccggagca cgacgtcttc gacctggacg
     4561 acgacgatgc cgccggaatt atgcgtcgct tcggccactt ccagctgttc accgatcatg
     4621 gcttcgcgct ggagggaaat gttgccgtcc tcatcggcca gggagccgcg gatgaagcag
     4681 acctggatgg gaaaggttgg ataatataag tagtcgtcgc cgtcgaacga gaccaggcgg
     4741 acgatatcct tgccttcgcg gacggtcttt tcgttggcct tgctgccgtc caggcgcgga
     4801 tcagccaacg tgtgcaggcc gatatcggtc cagacgccct tgtgaccggc ggcgatggcc
     4861 cgatagagag ccagtatcgt cccggccgga acgacatagg ccaggcactg attggctgcc
     4921 actttatcgg ccaacttctt ttccatgccg aaatggctgg taatgat
//
LOCUS       DAWABZ010000130         4897 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_4353_length_4897_cov_3.239777, whole genome shotgun sequence.
ACCESSION   DAWABZ010000130 DAWABZ010000000
VERSION     DAWABZ010000130.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4353_length_4897_cov_3.239777"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    432..441
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1061 a         1312 c         1322 g         1192 t
ORIGIN      
        1 accttcgcta aagaagcccg tgaagaaggc ttcgacaaaa tcgccttctt atttgaaaaa
       61 gtcgctgaaa tcgaaaaaca ccacgaagaa cgctacctcg ctctcctggc taacgtcgaa
      121 aaacaggaag tcttcaagaa atccgacaag aagacctgga tctgccgcaa ctgtggcttc
      181 atcgtagaag ctgtcgaagc tccgaaagtc tgcccggtct gtgcacatcc ccagtccttc
      241 ttcgaactcc gcaacgttaa ttactaatag tgaaaaaagg agctgtcgca ttgcgacagc
      301 tcctttttta gtggttcgtg gttcgtggct cgtggttagt agctctggct ggcagctctg
      361 gctggtgact agcttcgtag gggcttgcac gtggcaagcc cgccccagaa tatgagcgat
      421 tttccggcca gnnnnnnnnn naccaaatat ccagcgggtc gcccacgcgg cgacccctac
      481 agtaaaaaat aggctgtcgc atttgcgaca gcctattttt tttattcttc tacgcggaag
      541 cgataaccgt ttccccaaac ggtttcgatt gccgtgaatt ccggttcgac ttcgttgagc
      601 ttgtcccgca ggcggttgac gtggaccgtc accgtcgcca gttcgcccag ggcatcgaga
      661 tgccatatca tctcgaagag gtacttcttg gagaagacct ggttgggatg acgggccagg
      721 aacaagagca gattgaattc tttcccagtc agattgattt ctgtttttcc ccggaaaacc
      781 tggtggcgct tgatgaaaat gcgcaagtcg ccgacttgga tatccggttt gttgtcatcg
      841 gcatcctgga aacgtacccc taacagtctc tgatggattt ccagatgggc cttcaggcgg
      901 gccatcattt ccgatggatt gaaaggcttg acgatataat cgtcagcacc gagtcccaat
      961 cccttgatct tatcgtcgtc gctcccccga gcggtgatga aaaggatggg gacgttgctg
     1021 atgtcccgca gtttgcggca caacaggaag ccatccgtgt caggcaggcc gatgtccaac
     1081 aggactgctt cgatggctcc gccatcaaag atcttcaggc cttcctttcc cgtcatggct
     1141 acggatactt caaagccgtt ggcctcaaga aagtcccgtt cgatggcagc gatcatactg
     1201 tcatcttcaa taatcaaaac gtgcgccata actatacccc ctcgttgcgc tcccggtcag
     1261 gctatttccc cgtctgcaat ctccggaagc cgtaatctaa aatggtcggt gcatcggtcc
     1321 agcggttgtc gtcgttgtac aggaccaaga ccagcgtatg gccgtcgcgc gttgccgaag
     1381 caacgagaca ttcgccagca gcattggtaa agcctgtctt gacgccgttc gcgccgggat
     1441 agccgctggt caggaaatgg ttcgtcgtcg tgacgtgctt cggttcccga ccatccatgt
     1501 attttacatc ataggatttg agtcctacaa tataacgaaa ttcagggaag ttcttcatcc
     1561 cgtacgcagc catcttggcc atgtcgatgg ccgtcgtgtg gtgatgggct gccgtcaagc
     1621 cgttgggatt gacgaaattc gtatgggtag ctcccatttt gaccgcttcc tggttcatct
     1681 tttccgcata gccggcgaca gaacccccta aagtctgggc gacggcgaca gcggcatcgt
     1741 tcccggaaac gaccatcatg ccccgcaggg cttcacggat agaaatggga tcgcccggtg
     1801 caatccccag gcaggaaggt tccgtattgg cagcttccca ggaaatctgc aagggctggt
     1861 ccagcttatc cttatattgg tccagggccg taatgagggt gacgattttc gtcgtgctcg
     1921 ccgggtggac ccacttggtc ggatagacct gatataatac cttacctgta tcgacgtcga
     1981 tcatgcaggc cgattcagca atcgtcggcg gcatatcggc ctgagccgtc accgtcatgg
     2041 agccaatcat aaaaccgaca gcaatgaccg tcgtcagcat aatacgcttt aaaatagaaa
     2101 acatccaatg tctctttcct atctaacaaa attatgaatt gccagggaat acaaatccta
     2161 caataaccca ttatttttat tatattttta attaaaacat ctgtcaataa aaagaggccg
     2221 tcgtcatgcg acagcctctt tagtggctag tggcttgtag gggccgtcct gtccaggcgg
     2281 cccgccgcaa accattattt ttccggcttc acttcggccc ggaagatttc gtcgccaatg
     2341 gtcttgaccc agaaaccgat gaggttgcca taggtttcgc ggttcttgtc ttcttcggcg
     2401 gccttgatga tgtacgtcgt gaactggcgg gcccaggaat agccgaaacg gtcgaactgg
     2461 gccttgcggt tggcatcggc aatctgttcg atgatgggca ggaagatatc cttggtcact
     2521 tctttcaagt gttccgtaat gggatagaca taggccatgc ccttggcatc ttcctgcatg
     2581 aagaccaggg ccagcttgtc cggatcgtgg ctatcgtcgg catagtcgat gtgataggct
     2641 tccaggggca ggcgttcctt gtcttcctgg ctcaggagct gataagcgcc gtatttgatg
     2701 atttcataga ggcggtcatc ataagagcct tccagttcga tgatcttttc ccgccattcg
     2761 ccccaggtca gggcagcccg gcaggtatag gtcgtgaagg acggcgaacc cagggcatcc
     2821 tggacttcct tcgacgtgac ccgttctttc ggatccggga tgtgccagac caggagcttc
     2881 ttgtcccggt cgatgtagag gcacttgctt tccatgtgga tcttggcgtg gcaatgaggg
     2941 cacgtgtaga aaaatagatt ttcgtcgaaa tgaacgcgtt cgctcaattt cgcatcagcc
     3001 gtgtcgatga catcccacgt atggaagacg ccgtattcat gacaggccgg gcactggacg
     3061 gtgacgtcca ggtcatgggt tccgtttctt tttgccatgg gttacactcc ttccgcactg
     3121 ctggccggat acaaggccat gatctgcgat gcagcctgtt cgatgtctac gaggctgcgt
     3181 ttcctcaact tcaagaaagg atggacgatg gcttcgattt tcttgacctc ttccgacaag
     3241 gcaatcgggt ccggtgcatc atcgcggctc tgcagcaggg ccagggcagc gccggcttct
     3301 tccatcatgg ccgcctgttc attgccggca gcgtctccga gatggcggag ccagaagaag
     3361 acagccgctt tcgtataggc ttcctgttct tccgggctcg tgcagaccat gtcgatgcgg
     3421 tcgaagccgg ttccgtcggt caggagcaag gggacgacgg cagcggcagc gcgctgcagg
     3481 gggtagcgtt tttcttccgt actcagcgaa gcaaaagcga cgtgcttcaa atgcttttca
     3541 tcggccagat ggatggcctt cttataggcc gacaacaatt ccccttcttc accgcgcttg
     3601 ccgcctaccc agtacggccc gacagcgtgg atgatgaaag gcgccttcaa tttataaccc
     3661 ttggtcatga cagcactgcc gacgtggcac atgccgatcg tacggcacga ggcggaaaaa
     3721 gacaagccgc ctttctgatg aatcttactg ctgacgctat tcgttggcaa gagggtcgag
     3781 ctggcagaat atacgatccc gtcggccttc cactccgata aatcacctaa tactacacga
     3841 aactgcatcg tggtcacacc ctttccaaat ggatactctg gtcttattat accattactt
     3901 gacaaaggta ttaatagctt tcttgagatt ttctacggca tctgcgtcgt gttcacggat
     3961 ggcatcgatg atgcagtggt cgatatggtc cttgaggatg acccggctca caccggcgac
     4021 ggccgaatgg acggcggcca gctggaccag gacgtcgctg cagtcgcggc cgtcttcgat
     4081 catgcgcttg atggcttcga cgtggcccgt aatgcgcgac aggcggttga ggacacgctt
     4141 cgtttccgta tggtgatggc cgtgatggag gggatgggac gccacgccgg ccggctgttc
     4201 ctcgtcgtcg tagacgaaat cggcttcgtc agcctccttg acgactttga tgagatagaa
     4261 ccggtccgta tccatggcga tgacgacgga aaacccggcg ctgatcaatt tctcttccag
     4321 gacatcgacc ggcggcagca caggctggcc gccgtggatg gcgttgacgg ccttgcgggg
     4381 catatcgtgc atgatgagca gcgtcccacc cggcaccatg gccttataga ggcgacggat
     4441 gacgtcgcgg ctgtgactgg cgatatgggg ataaaaatta aggcagcaga cgacatcata
     4501 atgtttttcc gggaaatggt atttcaggac atcgcctgtc gtaaaagtga cattggccag
     4561 gtgagaaaat ttctctttgg ccacatcgat catctgctgg gaataatcga agccctcgac
     4621 ggtcccatca tcaccggcag ccgccgacaa atacggcagg agcacgcctg tcccgcagcc
     4681 ggcatcgagt atgaccgaac cgggccgtat ccctgccagc tccatgaaag acgacaagat
     4741 tttggcgtcg gccttgcggt cgcggtccca gttcctggca taattttcaa aaaatatatg
     4801 ttccttatcc atagggattc ccctttttga ttttatagta agcagtagct cgtggttcgt
     4861 ggctcgtggt tcgtggctcg tggttcgtgg ctcgtgg
//
LOCUS       DAWABZ010000131         4820 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_4416_length_4820_cov_3.481217, whole genome shotgun sequence.
ACCESSION   DAWABZ010000131 DAWABZ010000000
VERSION     DAWABZ010000131.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4416_length_4820_cov_3.481217"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    980..989
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1073 a         1362 c         1285 g         1090 t
ORIGIN      
        1 gtattatacc acatggtttg aaaatggcta tctctatgct acaatgtata gtaggtatca
       61 aaaggagcgg tcgtcatgaa tgaaaatccc ttatctcaca ttttggaata taatctggac
      121 ctgggcaaga ccaagcccga cacggtccat cgcgatgaaa agtattgtcc cttctgcgac
      181 gtagcccact tgaagaacgt catggaaaaa cggggcccta tgatctggct ggaaaacgcc
      241 taccccgtcc tcaaggattc gtggcagacg ctgatcatcg aaagcgatga ctgcgatggc
      301 gaattttcat cgtatacacc ggaatacgcc ttgaagctca tcgaattcgg cctggaaaaa
      361 tggcagcaag tcaaggacat gggaaaattc cggtccgtcg tcttctaccg caaccacggc
      421 tacatggccg gcggcaccat ccaccacccc cattcgcaga tcgtcggctt caaggactac
      481 gactatcacc aggacatcaa acctttccac ttcgtcgggc cttccatcat tgaagaagac
      541 ggcctgacgg tcaacttgtc ccagcgcccg atcatcggct tttatgaagt gaacctcatc
      601 ctcaaagacc ggggaaagct ggggaaattc gtccgctata tgcagctgac ggcagattat
      661 ttcatgcact cctttgtcag cttcaacgac agttataata tctttttcta tgacttcccg
      721 gctgacgatg ccttgtatgt caaaattatc ccgcgctttc tgaccaaccc cctctacgtc
      781 ggctatatga ttccccaaat cgccaacgga ggccattcag cgcgcttcat tcatgccttg
      841 cagcagcgct tgcatgaagt ataagaaagg acggttatta caactctatg cgtttatttg
      901 caatcggtga tctacattta tcaggcaatc cccccaaaaa gcccatggaa gtcttcggca
      961 gccgctggaa gaaccattgn nnnnnnnnng tcgtcctcat cgccggcgac acgtcttggg
     1021 ccatgcacct caaggaggcc caggaagacc tggaccagat acgggccctg ccgggtaaaa
     1081 aaatcataat ccgcggcaac cacgattact ggtgggaaag tgccggcaaa ctgaaccgga
     1141 tggacgccga gaataatatg cagtacctct acggctcgac agccgtcctg ggcgacaagg
     1201 acatcgccat ctgcggcacc cacggctggg tctgcccggg cgacatccac taccaggaag
     1261 accgcgacgc caagccctat cggcgggaac ttttgcgcgt cgaacggacc ttgcaggaag
     1321 ccgaggccct gcagtgcaag cacaccatcc tcctgctcca ctacccgccg gtctgcgatc
     1381 tgaccaagcc cagcggcttt acagaccttt tggaaaaata tcacgtcccg ctctgcgttt
     1441 tcggccatct ccacggcatg cagcctggga ccatgttccc gcgccgctac aacgggacgc
     1501 tgctccagct cgtctcggcg gactaccggg actgcaagct cttagaaatc aaattcgacg
     1561 gacaggaggc cttgcgatga gtcaaatagc caaaaaacac ctcttagccg gcatcgtctt
     1621 cggcgacgcc gaaacgggtg aatacatcta cctccctggc ggcgaagtcg ggaccgacca
     1681 gccgctctgc gtctttgaac acgacggtca aaaagaagac gtcgacctgg accgggccgg
     1741 ctacctcgtc gaccacctga ccctgaaaaa atgcagccac cccaccttag gcaagcggtc
     1801 gttctgatgg gaagaacccc tccaccgctt tgcggtcccc ctccccttcg caggtgaggc
     1861 tttcggctcc cctgcgaagg ggagctggcg ccgcaggcgt ctgaggggtt ctcttccaaa
     1921 aaaggctagt ggttagccga tggttttaaa tttaaagcca tcttctacaa actataaagc
     1981 aaatgggacc tcgcatgatg gcaaaaacct tcgtgcgagg tcccattttt tatcccttac
     2041 aacgacctac gatgaacgac gagctatctc ttctttatgg tgaaccagaa ggtgacgccg
     2101 tctttttcgt tgcggacgcc gtaggtttcc ccgtggagtt tcaccagggc cgcgacggtc
     2161 cccagaccca ggccgtggcc gccgaaaacg cgtttcgggc tgcgggcccg ggatgaatcg
     2221 atcttataga acggttccca aatgcgctgc tggtactctt cgccgatatg gctgccttca
     2281 ttgtagacac agatgcggta gcgcttcccc aggtcatcgg caaagacgtg gatatgcctg
     2341 ccgacgggcg tatagtcgat ggcattgctc aggaaattgg tcagaatcgt atcgatccgt
     2401 tccgggtcgc catagacgtt catttcagct ggcagttccc acttggccag gatatgctta
     2461 tcagccagag ccttggcaaa gcgggccttg ctttcttcgg ccagggccgt gaaatcaaag
     2521 accgtctgtt ccagtttgaa agagtccgtt tccagacgcg acaagttgag caggtccttg
     2581 acgagtttat ccatcttctg ggtttcactg cggatgacgg cacagtagcg ctgctgcgtt
     2641 tccttgtcga tatccagcgt gtccagccct tcggcatagc cctggatgat ggccagaggc
     2701 gtcttcagct catgggagac agccgacagg aaggacttgc gcatgtgttc cacttccttg
     2761 gccttgtcga gctgcttctg caattccgta ttggactgct gcagagccgt cagggcgacg
     2821 ttgagctggt tggccagcgt attgatactg tcgccgagct ccccgatttc atcattgaaa
     2881 ttccccttcc agcgcttgga aaaatcgagg tgagccatgg cgacggccag gttcttcaat
     2941 tctagcaggg gctgggtcat gcgctgggtc aagagaatcg taccgataat agccaggacc
     3001 agccagatga ggccgcagac gatgatgaac cgctggacga tgtagatatt ctcccgcaag
     3061 ggtgcgacgg actggctgat aatgacgtaa gctccgtctt ggacgcggtc gatgagattg
     3121 aaatgctcga aatcaggcgt atccttatag agctggacct tgcctagcac ttcttcttca
     3181 ggcatcttgc catcgagcaa atcccgtagt tcggcgatat gcaggggcag gcggaagacc
     3241 ggcttgtcag cagccgcttc attttctttc ccttggacga tgacttcttc atcttgtata
     3301 tctttttcca taggcagatg gacccgccgg cctggccggg acgaatagac caatttcccc
     3361 tgatcgacga tgagcacatc caaggcatac tgctggctca ggtcgtccag cgaactttca
     3421 tctgaaccgc cctctttccg gtagatattg ctgattttct gactcgcttc gaccatagct
     3481 tctttcttca tggaataata gtagcgttca aagccgaagc cgacggcaac catgagagtg
     3541 ataatgaaga aaatcgtaaa aaagccgaca tagaggccgg ccttaaattt taacgaccag
     3601 cgccctttcc tcatttgcca tcatagcggt aaccgtagcc gcggactgtg cggatatagc
     3661 ggcctttatc gccgagcttg atgcgcaggc ggttgatgtg cgtgtcgacg gtgcgcagat
     3721 cgccgtagta atcgtagttc catacctggt tgaggatctg gcggcggctc agggccttgc
     3781 cggtattgct catgaggtag ttgaggagct tgtattccgt cgggctcagg tcgacgttct
     3841 tgccgtcgag gacgacctga cgggcatcgc ggtcgatagc gacaccctca tcggtttcat
     3901 gcgtttcctg ttcattctgg gaacggcgca ggagggcctt gatcttggcg actaaaatct
     3961 tcggactgaa gggcttggtg atatattcat cggcactgag gtcataggcg aagagctggt
     4021 cgatttcttc ccctttcgcc gtcagcatca tgatggggac cgtcgattcg cggcggattt
     4081 cgcggcagac cgtccagcca tcataaccag gcatcatgac gtccagaagg atgaggtccg
     4141 gatgttcctt atcatagatt tccagcgctt ctttgccgtc tgacgcttcc aggatttcat
     4201 agccttcggg tctcaagaag tcgatgacca attgacgcat gagctgttca tcatctgcaa
     4261 tgagtatttt ttccatagtt actgtccttc tttctgatag agtctgtatc cggcaaccgg
     4321 tccagccagt cgccaatggg ccgaccgcaa aggatgaggt ccggcgccaa gtcccgcaaa
     4381 gggaccagga cgaaagcccg ctgtgtgaga taagggtgag gcaagatcag cgtttccgtc
     4441 tcacacgcct cgtccgggct gtagacgagg tcgatatcga tagtccgggc gccccatttt
     4501 tcgcggcgca cacggcccaa acgccgttca atggccagac aggcatcgag cagctcctgt
     4561 ccggaccctt ccgtttcgac agccacggcc ccattgatga aggctggctg gtcggtcttg
     4621 ccccagggcg gtgtttcata aaaaggcgaa acagccgtga ccgtcacgcc cggcgtatcg
     4681 gccaggaggc ggacggcctg gcgaagcgtc gccaggcggt cacccagatt ggcgccgatg
     4741 ctgagataga acttagccat ggcgccgcct ttccaggacg acggcagcgt cttcaaaggg
     4801 cccgccgatg ggcgcctgcg
//
LOCUS       DAWABZ010000132         4809 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_4430_length_4809_cov_2.889987, whole genome shotgun sequence.
ACCESSION   DAWABZ010000132 DAWABZ010000000
VERSION     DAWABZ010000132.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4430_length_4809_cov_2.889987"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    474..483
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1073 a         1403 c         1231 g         1092 t
ORIGIN      
        1 ctttaggaca agaaattaga tattatcgca aaaaaaaggg cttaacccag acggctttcg
       61 gtgccctctt tggcatgtcc aaacaagccg tctattcttg ggaaacgggc ctctattcac
      121 cggatatctc tgtattattg aagatggctg atttcttcca gattcccatc tgtatcctcg
      181 tcggccggcc gggcatgtac tgcaaaggca gcgaagagca cggcaacagc tgcgacttct
      241 gtgcttccat caccgaagac gacagccaca tcatccgggc catccacgcc ttgccgccgg
      301 aaaaacagga ggccattaaa acattattga atatcaatgg caaaaaataa agtgttacaa
      361 aaagaggctg tcgcattaag acagcctctt tttgagtggt tagcagctag cggatggatt
      421 taaatttaaa gttcatgcaa atccgattca caagtatctc acatatcccc tggnnnnnnn
      481 nnncccccac gagccacgag ccactaaacc atggtatgat ggaagaaaaa tgtaaaggac
      541 gatgtgtgtt atggaatctc tcatccgctc ggtctacggc cgggccgacg ctgaatatgt
      601 catcaagaag tcccgcttca tcgcgacggt ctgcgaagtg aaatccgaag acgaggcagc
      661 agccttcatc gaggaaatga aaaagaaata ctgggatgcc cgccacaact gctcggccta
      721 ccaggtcggg ccggacggcc gtttccagcg ctccagcgac gatggcgaac cggccggtac
      781 ggcaggccgt cctatcctgg aagtcctcaa gaagcagggc ctgaccaata cggccatcgt
      841 cgtcacccgt tatttcggcg gcatcaagct cggcgccagc ggcctcatcc gggcttacag
      901 ccacgctgcc gccctggccc tggaagccgc ggatatcgcc gtctatacgc ccttcacgat
      961 tctgacggcg accgtcgcct atcccctggt cagcacgatg gaacgcttcg tccccgacca
     1021 cggcggcacc attaccggcc gggatttcgc cgccgacgtg accttcacca tggaagtccc
     1081 gcaaagcggc agcgacgcct ttgccgccag cctgaccgac acgaccagcg gccgcgtcac
     1141 ctgccggaaa accggcacca tcgtcaagcc cgtggttagt ggttaggggt taggggctag
     1201 cttcctttta ctctataagt gtagtacaga gagtttgtag ttcgcagaga ggactttaaa
     1261 ttcaaaacca tctgctaacc actagcccct aaaaaaatgg cttgtcgctt agtgacaagc
     1321 cattttctca tgctttatac ccggtcatga ggtcgacgat gttttccaga gggtcgccgg
     1381 cccggtagcg tttgagatta tctgcggcga tgcggacgat gtagtcgtgc gtcgcccgga
     1441 gatgatagcc gccggagatg tgcggcgtaa tgtagatgcc cggcgtcttc cacaaggggg
     1501 ccgacgacgg cagcggttcc gggtcggtaa catccagggc agcgccggcc agatggccct
     1561 gggcgacggc ttcctgcaaa gcggcctggt cgacattggt gccgcggccg acattgacga
     1621 aataagcgcc tttcttcatg gccgcaaagc gcgcggcgtc atagatatga tacgtttccg
     1681 gcgtatcggg caggcacgaa gcgacgatat ccgctttcgc caggtattca ttgaaatggg
     1741 ccatatcccc catttcgtcg gcttccggcg gtacggcacc ctgacggcgg cgcaagccga
     1801 tgacgtgagc ccccatggcc ttgacgcgct ggccgaagtt gcggccgata ttgccaaaac
     1861 cgacgacgac gactgtcgcg ccgtagaggg atgtgaccgg cccttcgtcg tgccacaggg
     1921 cctgatgctg attgtcatag taaagataga gtttcttcat catagccagt gtctgggcca
     1981 gcatgtgttc cgacaaggcc aggccatagg caccggtcgc attggtcaag gtgacgccgg
     2041 ccggcaggat accgggctgg cagtattcgt tggcaccggc actgttgagc tggagccatt
     2101 gcagctgcga ggccttcttc accaggtcag gagcgatatt gccaatgatg acgtccgctt
     2161 catccagcgc cttgccggcc tgggtcccat caccgggaca atagcaaaaa tccgcttccg
     2221 gagcagctgt ttccagccac tgtttctgtt ttgcatcgac atccatgaca actaaaatct
     2281 tcaaacgaat cactccctat tgcttttttt gtttagcggc aatgcgtttc accgtttcca
     2341 acgggcattg ggcaacgtgc gcaatgaatt cataggtcac gtcttccttc aataagttcc
     2401 tgacgatctg atcgacactg caccgttctc cgtccgcatt gacgaattgg cccattttcc
     2461 ccgcctcctc tgccattata acacaatcga gagacctgca tatatattgc aacaaaaatc
     2521 atccttcttt cttgaggagc cgctaccagc tatttgcaag ccaaaagata gcagccgcct
     2581 atttctattt cattatagct tctcaataaa gaaaaagata gcattccacg atagctgtaa
     2641 cagaactgtg caggcccggt cataatatgg ctgatgcgtc gtaatctgtc ttcactgttt
     2701 ttcccggatt gacaagcgct atttttgttc tatactaata ggcagagtgt aatcgtcaaa
     2761 cgaccgcacg aaggggtaca ccaccttggg tgtaaacttt cgtgcggccg ttttttttgt
     2821 taggaggtct ttttcatgaa agaagcatca cagatgcagc gcggcctgaa gaatcgtcat
     2881 ctccagatga ttgccctggg cggcgctatc ggtacggggt tgttttatgg gtcggcgtcg
     2941 accatttccc tgggcgggcc gtcggtcatc gcagcctatc ttatctgcgg catcgtcatt
     3001 ttcctcatca tgcgcatgtt gggggaaatg gccgtggaag aaccggtgtc cgggtccttc
     3061 agttattacg ccgacaagta ctgaggccct ttcccgggtt tcatgctggg ctggaattac
     3121 tggttctctt atgtcatcat cagcatggcc gaattgacag ccgtcggcat ctacgtccat
     3181 tactggctgc cggacctgcc gcaatgggtg acggccctgg tctgccttat ggccatcacc
     3241 tgtctcaacc tggttaatgt ccgggcgtac ggtgaaacgg aattctggat gtccttcatc
     3301 aaagtggcgg ccatcctggg catgatcgcc ctcggcgcct ggctcatctg ccaggacagc
     3361 cggcccttcc cgcagaattt cagcaatctc tgggattacg gcggcatcct gccaaacggg
     3421 atctggggct tctgcctggc catggtgacg gtcatctttt cctttggcgg cgtcgaactg
     3481 attggcatca cggcgggaga agcagaaaat cccgaacggt ccctgcccaa ggccatcaac
     3541 cagatcatct ggcgcatcct cgtcttctac atcggcacca tggtcgtcat catgaccttg
     3601 tggccatgga accagattgg cacacagaac agtcccttcg tccagatttt cactaacgtc
     3661 ggcttcccgg cagcggccaa tctcctcaac ttcgtcgtcc tggcggcagc catttccgtc
     3721 tataactcgg ccatttacgg caattcccgc atcgccttcg gcctggcaga agaaggcaac
     3781 gccccgcagt ggttcaagaa gctgtcgtcc cggggcgtgc cggtccacac actctgcctc
     3841 tgctcgggcc tgaccttgtt cgtcgtcgcc gtgacctact tcttcccggg aaaaatcttc
     3901 atgtatttcc tgtccctcac cgtcggcgcc accatcgtca actggatcac catcatcgtg
     3961 acgcacctga aattcaaggc ctactgccag aaaacgggca agaccacgaa gttcccgtcc
     4021 ctctggtatc cggcagcgga ctatttctgc ctggccttcc tctgcttcat cgtcctcatg
     4081 atgacccaaa tgccggacat gctcacagcc gtcctcatca tgcccgtctg gctggctatc
     4141 ctctggtgct gctggcgccg cctggcccct gcgccaatga tgacacacac catgcaaaag
     4201 ccttagcaca acgctgccca gtctgttgga aatggtttcc cttattccat tccaacagca
     4261 cctgcatgcg ggacaattct actaaacagg catactgctg acgaatacag tcccctaccg
     4321 ccgccatgac aggattgcgc gtcctttcct ctttgtcgcc caggctcaag tatacggcct
     4381 gggtctgcgg cggccgctga ctggcataat ccagccaatg gggaaaccag accgacggcg
     4441 acgcggcagc aatagccgag aaggctggcg tctgccaggc gcaccacaaa gcaaagagcc
     4501 ctgccaggga atatccgcct aaaataacag ggatgccttc aggtaaatgg tatcgcaggc
     4561 ggacagctgg caagagaact gtttctacga agtgtagtgt ttcggctgca ccatgcccaa
     4621 aatcagaaca cccaaaaaca gcaggactat cccagggaga taattcctga ttccaatctt
     4681 ttaccagaaa agcgacaaaa acgaaaggta cttggacagc cttcgcaatg gctgttacct
     4741 gttcgtccaa tacctcccaa tcatggtcat caacaggctg tatcaataga tacaccggct
     4801 gccctgctg
//
LOCUS       DAWABZ010000133         4798 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_4437_length_4798_cov_3.453089, whole genome shotgun sequence.
ACCESSION   DAWABZ010000133 DAWABZ010000000
VERSION     DAWABZ010000133.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4437_length_4798_cov_3.453089"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1173 a         1261 c         1087 g         1277 t
ORIGIN      
        1 gttcgcagct atccgtaacc gcattggtaa tagcatccat tatttgaaaa aggtcagcct
       61 gaggaatacc gataacccgc atcgttctat cttcaacacc aaatacaatt cgtccaccat
      121 cgccattggc aaaggcaaca acagacttca tatatttctg gctgtcttca ggaacccttt
      181 ccttatattc tatattctta gactcccctt gaagcaacga atcttttttt atatctgtca
      241 cctgtttcac ctttgtcact ccttcgatgt atcattatga ttattatacc ccatccgtga
      301 cagggacgac aatgaggaag ggccgcagcc cggaaaaacc actcacggac gcaggaaaaa
      361 aacgccggcc aggcacagga tgatgccgat gatctgccgg ggcagcagaa cgtctttgta
      421 aaacaggacg cctatacctg ccaggaggag cgccacgctg acattggcca ggagagaggc
      481 catactgatt ttccagccag tccggtatag caggatatag ccaaattcga cagccaccag
      541 cgtcagtccc caggcagcgc ttgcccagtt caatgtatgc agatcctgcc gcagcgaaag
      601 ccctgtcacc acgtaagcag cggcacacag gaggaaactg accagatagg taacactgag
      661 ggccagaaaa ggcggggctg ccgacggtgt tgccttggcg accaggtgat agcctatatt
      721 gccaatgacg atgatgaaaa aaggaatcca gtgtatcagg ctcattctgt caactccttt
      781 tccatatgga tgtgaggaca atattcatcc agatattcgt cacctgatgc ggtatatccc
      841 agtttttcat aaaacgggcg cacccggacc tgtgccgaca gagccagccg tttcacatga
      901 cggccgcgca gccatccttc cgctccctgt accaggcgct ccccgtactg tcggccgcgg
      961 caggatttcc gtacggccag gcggcccagt atatacacat ccggattgtc tgatgtaaag
     1021 agccggcagc agccagcggc atgcccattt tcatataaca ccagatgcca tgccgcctgg
     1081 tcggtttcat caaattcatg atgaaaaccc tgttcttcga caaatacctc ctgccggata
     1141 atccgggcgt cctgtgaaag tccctgctgc atacgtattt gaatcatgat attccctcct
     1201 aaaaaaaaac agaggtgccc aggaattgtc ttatagcatg taatccatgc cgtacctacg
     1261 acaactccca gacacctcat ttctcctgtc cggtgacaga agcggtgttc ttatgaaatt
     1321 agcattgttc taacatatcc tgtcgtcttc gtcaatgtga ttttgcccac tttagcagct
     1381 gggaaacaga ccatcctccc gacgtttctg cctgttattt caaggtctgt tcggtatctg
     1441 ccagaaactg ctgcacacaa tcggcaaaat ccagcccagt ccggtcggcc agaacggaca
     1501 accaccatac acattccccg attttagacg ggagtttgcc ttcttcatca gatggccagc
     1561 gcccttcatg actcatagtc agccgtccta ccaggccggc atcggtcaaa aaagccaggg
     1621 catcttcctc gacggaccat tcggagccat gatactcccg ttccaatgca tgataagcag
     1681 ctctgatagc agccgaacgt tccgatactt cttttaaatc cacgtcaatc tctccttttc
     1741 ttagaaacac ggccacaccc cattcaatcc taatgacggg ccaaaaaggc ttaccaggcc
     1801 gtcccctaca ttatcacata taggcgataa aaataataca ccgttcacgg gcagtcgcaa
     1861 aaaaaaacac ggcctgcggc cgtatgcgta ggggctgtcc cgatgagggc agcccgctgt
     1921 gagatttctc cacaacccag tctggcatag tggcaggctt ttacggacta cgaatcacga
     1981 gcaacgaacc acctatcatg gatccggcta tgtacagaat tcatgcggga tcgccacgtg
     2041 cgagccccta cgatgcggct gcgccgctac aggcttatga cctgattaca accataagaa
     2101 cacctcagac cgcctttggc ggccagctcc ccttcacatg ggagcctctg gcgctcctgc
     2161 gccttatatt tcagcgttta ttgcggcaaa gccacttttg agtccgggca acaaaaaaag
     2221 acctgtgtac gtaatcgtac acaggtccga taatttccct tgtgaaatta tttgagttcg
     2281 acagtagcgc cagcttcttc gagtttagct ttgagagctt cagcgtcagc tttagcaacg
     2341 ccttctttaa cagctttcgg agcgccgtca acgagagctt tagcgtcttt caagccgagg
     2401 cccgtaactt cacggacaac tttgataacg ccgatttttt tgtcgccagc ggactggaga
     2461 atgacgtcga attcggattt ttcttcagca gcagcaccgc cagcagccgg agcagcagca
     2521 actgcaaccg gagcagcagc ggaaacgccg aatttgtctt ccatagcttt tacgagttcg
     2581 gaaagttcga gaacagtcat attttcaatg gcttccatga tttcttcttt agtcattttt
     2641 aaatacctcc atagtatcaa tttttgagtt taatttttaa gttatgtaag ctaaattaag
     2701 cagtttcctg ttctgctttt ttagcacgta cagcgtcaag cacataaacc atgttgcgga
     2761 tattgccctg gagtacgttg acaaggccag agataggagc ctgcatgctg ccaaccaatt
     2821 ttgcgaggag ttcttcgcgg ctcggcaagc ttgccagagc gtcaacagct gcaacatcaa
     2881 tgacctgacc atcagcgaga cctgctttta cagtcagcgc ttcagtcttt gtttctttga
     2941 tgaattcttt gaggatctta gccggagcta cagcgtcttc cgtggaataa gcgagagccg
     3001 tcgggccttc gaaaattgca tctaatgcgt tatagccgag ttcgtcagcg gcgatacggg
     3061 tcaacgtgtt tttgatgact ttgtattcaa caccgccttc gcggaactta cggcgaagtt
     3121 tcgtgacatc agctacggta aggccggaga aaccaacgaa aacagcgccc tgagcggact
     3181 gcagtttttc tttcatttct tcaacgacag cagcttttgc cggagaaacc ggatgatttg
     3241 catatgtgga catgagttcg atacacctcc ttgaactggg atttatatcg tcttagcaat
     3301 caaaaacggc ctcatggaaa ccacgaggcc gaactagtca aagtcagata tctgctagtt
     3361 tcatagcctc agatggcgga catacgtcca ttatacatac cttcagtcta cagctaagag
     3421 agatattaat tttcttcttt ctttgcagca cctttgatgc tgtactggtt gatgtggatg
     3481 ccagggccca tcgtggagct aaccgtgatg gatttgatgt tctggccttt tgcacccgac
     3541 ggtttaactt tgatgagtgt atcaaccaaa gcagtcaggt tgtcaaccaa tttatctgcg
     3601 tcgaaagatt ttttaccgat agaagaatga atgttgccgg ctttatccgt gcggtattca
     3661 actttaccag ctttactttc gctaacagct tttgtaacgt ccatcgtgac agtaccaacc
     3721 ttcgggttag gcatgaggcc tttaggaccg aggattttac cgagacgacc aactttaccc
     3781 atcatgttcg gagtagcgat tgctacgtcg aagtcgagcc agccaccttt gatcttttct
     3841 acgagatcat cagcaccgac atagtcagcg ccagcagcta aagcttcttt ttctttatcg
     3901 ccctgagcaa atacgaggac agtcttggat ttaccaatgc cgtggggcag taccaaggcg
     3961 ccgcggacct gctggtcggc gtatttcgga tctacgttaa ggtttacggc caattcaacg
     4021 gtttcgtcga atttggcagt atccatcttt ttcaagatgt cgattgcttc tgcaacatca
     4081 tactgtttct gtttgtcgaa cagttttgca gcttcagcat atttcttgcc tacttttgcc
     4141 atgtttcttc ctccttatgt ggtttaacgg gttacccctt ccacgggcag agcatcatcg
     4201 aattagtcga cgatttcgat acccatgctg cgagcggtgc cttcaatcat tttcatagca
     4261 gcttcaacgt cgttagcgtt gaggtccggc atcttagtcg ttgcgatttc gcggactttg
     4321 tcgcggccca atttagctac ttttttcttg ttcggttcgc ccgaagcttt gtccaaacct
     4381 gctgctttct tcaagaggac ggcagccggc ggtgttttcg taatgaaagt gaaggaacgg
     4441 tcttcataga ctgtgatttc gacagggata atgaagccag cctgtttagc tgtgcgttcg
     4501 ttgaattcct tgacgaaagc catgatgttg acaccagcct ggcctaatgc agggccaatc
     4561 ggaggcgccg gagttgcttt accagcttca cactggagtt ttaccattct tgctactttt
     4621 tttgccatgg tgagttacac ctccttacat tacaagatgt ggtcaaacga atcttgcgat
     4681 tctcccactc gcggcgagtt gccgcattta gaagtattta ttccagaccc ttctcgattt
     4741 gagaatagtc gacttctact ggagtttctc ggccaaacat attgacgagg cctttgac
//
LOCUS       DAWABZ010000134         4769 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_4467_length_4769_cov_2.616249, whole genome shotgun sequence.
ACCESSION   DAWABZ010000134 DAWABZ010000000
VERSION     DAWABZ010000134.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4467_length_4769_cov_2.616249"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1177 a         1334 c         1261 g          997 t
ORIGIN      
        1 cctttacagg ccggcctcta cggtggcccc ggatggggca attttacgag ccaatggttt
       61 cccgaggcga tgtcgtccct ttgagtttga cgccgcgcag cggcacggca tttgagtcca
      121 tggcggcctg agatggcggg gccgtcaggc cccattacac gcgccctttt caataaaaat
      181 aggagagaat tactatgcaa aaagttacta ctgccgctca gatgcggaat atggatcgga
      241 tagctatgca tggcatatat gccattgcgc cggctgtcct catggaaaat gccggccatg
      301 ccgttgccga acgggccggt gcctggctgg aaggctggga tggcaaggat gtcgtcctct
      361 tttgcggcaa aggcaataac ggcggcgacg gcttcgtcat tgcccgccat atcttagccg
      421 ccggggccag ggcctatgtc tatgtcctcg gggaagaagc cgactatagc gatgaagcgc
      481 gtcagcactt gcatacgctg cagcagctgg ccgacgatga aacgtgcctg ctcttgccgg
      541 cgccgcagtc ggaagctgac tggcaggtcc tgcaagaacg gctcctcgcc tcttctgtcg
      601 tcatcgacgc catgttgggg acgggcttcc acggcagcct gcgccagccc attgcctcta
      661 tcgtagcgga agtcaacgcc gctgccgccg ccggtgccgt cacggtcatc gccgtcgata
      721 tgccgactgg cgtcaactcc gatacggggg ctgtcagcaa cggtgacgac gatgaagacg
      781 gtccgctctt tgccgatatg accgtcacct ttggtgccct caaacggggc ctggtcctct
      841 atcccggccg ggcttgtgcc ggccacgttg aagtcgaccc catcggcatg cctggtccgc
      901 tgctcatgca gctcgaagaa gacccggctt atttgctgca ggaatcggat atctctgaaa
      961 tcgtcgtgcc ccgcagcccg gacagccata aaggaactca cggtaccatc gctatcgtca
     1021 ccggcagcag ccaaatggcc ggagccgccc tcatggccgc tcatggagcc gtccgcagcg
     1081 gggctggcaa ggtcttcctc cgcgtgcccg gtgcgacggc gccatactgc atcggcgccc
     1141 agccggaaat catggtccgc ggcgtcggta ctggcgacca cttcactgct gctgatgccg
     1201 atgaaatcat tgctgaaagc aagacctggt ccgtcctggc catgggcccc ggcatgggcc
     1261 gcgacgccgg gacgaaagaa tttctcctga aagtcctggc cgctgtagat tgcccggtcg
     1321 tcctcgatgc cgatgccctc aacctgctgg ccggagaaaa ggccctcatc gcttcgcggg
     1381 gcagccggat tatcatgacg ccgcacttgg ccgaattttc tcgcctcagc ggcctgtcca
     1441 tgacggaaat caaagcggac cccatcgcgg cagcccgtca ttttgcctcg gaatggaaag
     1501 tcaacctcgt cctcaaaggg gcgccgacgc tcatcgtttc ggcccggacg gggaacgtct
     1561 acatcaactc cacgggcaat gccggcatgg cctgtggcgg catgggggac gtcctgaccg
     1621 gcatgattgc cgccatgacg gcccatcagg gcatgagcga tttgtgcagt gccgcctgtg
     1681 ccggcgtcta tctccacgga tcagccggcg atgcctgccg caaagcctgc ggcccctatg
     1741 gctttacgcc catggaaaca gccgatgccc ttcccggcgt cctggcttct ttagaagaag
     1801 atttggccct gcccattttg cagcagccct tgattggagg aaaataaatt gaaaaaaaga
     1861 aaactcttta ccgccgccct ggccctggcc atgagcttca tgctcgtcct ggccggctgt
     1921 ggcaacagcg cgtcgtccag ctcgtctggt ggggccaagg ccgtccagtc cctgagtgcc
     1981 tctgacaagg acctgcgcat atccgtcctc aacgtcggtc aggccgatgc gacgctcatc
     2041 cagtataaag gcaagaatat gctcatcgat acaggcgaag tcgacagccg ggccgatttg
     2101 gtcaaacagc tcaaagaacg caaggtcaag gatatcgaca tcatcgtcat cactcatccc
     2161 cacggcgacc acctgggcgg catggcggaa ctgttcaagg acttcaaaat caagcagatc
     2221 tatgacaatg gccagagtgc caatacggcc atgtaccgca attacctgaa gaacatcaag
     2281 gccaagaata ttgcttataa gaccttgaaa aagggcgata ccttcaccct gggtgatgac
     2341 gtcaccttta ccgtcctgtc gccgggcacg cctttcacta aagaaaatac ccagagcgtt
     2401 tctgaaagcg gcctgaccaa tgacaattcc atcgtctgca agatgaccta tggccagttc
     2461 tccatgatgt ttactggcga tgcccagaaa gaagtcgaag aaaagatcct caaggactat
     2521 aaaccagctg acctcaaatc ggacgtcctc aaagtcggcc atcacggcag caagacctcg
     2581 tcgtccgacg ccttcgttaa agccatagcc ccgaaagcgg caaccatttc ctgcgccgcc
     2641 ggcaaccagt acaagttccc gcatgaaccg acgctgaaga ccttgcagaa atacaacgtc
     2701 gaagtctatc gcactgaccg caacggcgtc atcaccatta ccaccgacgg ctcgtcatac
     2761 agcattgaaa aggagtattg atatgaaaaa agaactcgtc ggcagcatcg accgcataac
     2821 cgaacagaca gcctacatca tcctcaacga cgatgaacac gaactccagt tcccactgga
     2881 actcttgcca gacggcgccg atgaaggcat ggccttcact atcaccatcg aacgcaacga
     2941 ggaagaagaa aaacgactgg ccgaagaaat cgaggccttg aaagaaggcc tctcccaata
     3001 gggagaggtt ttctttttgc tcttcccagg attcacggcg ggctcgccac gtgcgagccc
     3061 ctacggaatc gcatacaggc gatgggaatg agtgcaggcc gcaaaaaaaa tacacggcca
     3121 gcggggccgt caggcccctt atatatattc cctaacacgt tttactaaac acacataatg
     3181 catgaaaata aataattact acaggaggta attttatgat tgggtataaa aaaatcgcag
     3241 tcatcttggc cgttttttcg gctatggggc tggctgttca aggtcaggaa aatggcgata
     3301 gcatcgcgct ttctacgaat gtacccacgt ctttgccggc tttggcgccg gcagcgtatc
     3361 gcggtcattt ctggcgtgtc gatgcagaaa attatgcagg tctgccacgg aattttcgca
     3421 cttgtcagtc atcttttcac ggagtggaac ataaatatgc atctgaggtg gatccgtctt
     3481 atgttccgtc ccggaagggc ttggatgacc tgcgtatttc cgggtccggc cagttttcgg
     3541 cccgccaatt cgacgccctc gtccatgagc tgcggaaaaa gaccaagggt cctatttatg
     3601 acgtcgactt gcgccaggag tcacacggct tttttgacgg tacggccgtc agctggtatg
     3661 gccgtcacga ttggggaaat ataggaaagt cccaagccga ggttctcatc gatgaacagc
     3721 agcgcttgca ggccgctttg gggacagacg tcacggtcta cgaccagggg aaaggcgatt
     3781 tgcccgttca tcctcagatt atgaccgtac gacgcgtcca gacggaacag gaattggcag
     3841 aaagcaaggg cgtccactac gtccgcctgg ccaatacgga ccatctctgg ccgacaccgg
     3901 acgaaatcga ccgtttcctg gccttcgtcc gcactttacc agacgatgcc tggctccatt
     3961 ttcattgcga agccggagcc ggccggacga cggcttatat ggtcatgtac gatatgataa
     4021 agaatcccga cgtgtcctac cgggatatcg tctatcgcca gtatgaaatc ggcggcaact
     4081 acacgccaca tgacgtatcc cgtcccaaac gaggcgattg gaaaggaccg tattatcatg
     4141 aaaaacacga aatggtgtcc ttattttatc agtatgtaca ggaccagacg aaacagggat
     4201 ggagccagtc atggtcacaa tggttacaga ataaaagaat gagagtcaat aacgtttata
     4261 acgataacga catataaaac gacaagaaat gtataaataa ccatttacaa aatttttatg
     4321 caaaaataag ctttaggaaa tcaaaaaata agactaaaaa atggaagctg aatggcgctg
     4381 ttaagccaat ctttatttcg ctaaaactgg tttgcagata tagagttttg acaagcgccg
     4441 ggaaaagttg tataataatc gcaacaggtg tggtgcggat gcaggcaaca cctgcggaaa
     4501 gagcaaaaaa acagtacatt tttcaaggca gtaaaagaga ggaaactagg acaactcgcc
     4561 aatgaaccct tgaaaaaata cgtttcgacg ctcgctagta acggtgtaag gcttttaacg
     4621 aaacttattt tttattggaa aaggggtaat tctcatgaaa tccaaagtat tagcagctct
     4681 cgtagcaact atggctgttg gcgcaacctg cgccttcgca gctaacccgt tcgtcgacgt
     4741 accgtccgac agctgggctt acaaatccg
//
LOCUS       DAWABZ010000135         4768 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_4468_length_4768_cov_2.839593, whole genome shotgun sequence.
ACCESSION   DAWABZ010000135 DAWABZ010000000
VERSION     DAWABZ010000135.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4768)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4468_length_4768_cov_2.839593"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1176..1185
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2792..2801
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1186 a         1232 c         1218 g         1112 t
ORIGIN      
        1 acatgaaaga accgcaggtc gttgttcttc cggctactac tgaagaagtt tccaaagtcg
       61 ttaagctctg ctatgaaaac gatacggtca tggttcctcg tgcagctggt acgggcctgg
      121 cagctggtgc tgttgctacc ttcggcggcg tcatcatctc cattgaacgt atgaacaaaa
      181 tcctcgaaat cgataaagac aacatggttt gtgtttccga agccggcgtt accacgtctg
      241 tcctccagga tgcagtccag aaggaaggcc tcttctacgg cggcgacccc tgcagtgggg
      301 actcctgctt catcggcggt aacgttgcaa ccaacgctgg cggcaaccgc gctgttaaat
      361 atggcgtaac tcgtgaccag gtcatgggca tggaagtcgt tactccgact ggcgaagtcg
      421 taactctcgg cggtaaattc cgtaagaacg ctacaggcta catgctgatg cacctcttca
      481 tcggttctga aggtaccctc ggtatcatca ccaaagttta cctcaaattg gttgctcttc
      541 cgaaatatca gcaggacctc ctggttgtat tcgacaacct cgacgatgct atcgctttga
      601 caccgaaagt catgaacgct ggtatcactc cggtctgcgt agaattcatg gataacgctt
      661 ccatcaaaca ggtcgaagta ttcctcgacg aaaaactgcc gaaatctgaa aatggtaact
      721 acattatcat ccagatcgct ggcgacaacg aagacgtcct catggatcag tgcgaaaaaa
      781 tcgttgaact cgctgaagac aacaaggcta tcgactccct ggttgctgac ccggctgtta
      841 tttggaaatc ccgtaaagca tacctcgaag ctgaccgcgc tcgcagctta gtcttctcca
      901 tggaagatat cgtcgttccg atgacggaaa tcacgggtgc tgtacagcag atttcccgcc
      961 tcggcgaaaa atacaatgtt gctatgcact gcgctggcca ctgcggcgac ggcaacgtcc
     1021 atatcgatat cttgaaagat gaccgtactc aggaagaatg ggaagaaatg cttccgaaga
     1081 tccagggcga aatctacgct ctcgtctaca aactcggcgg ccgtctctcc ggtgaacacg
     1141 gcattggcta caaacgggaa gcacttcttg aaaaannnnn nnnnnctgag ctgccgttca
     1201 tggccagcga gaatatcatg atgaaagctg tcaagaaagc actggatccg aagaacatca
     1261 tgaatccggg caaagttgtc agcatcaacg acgaagtccc tgtcgaagaa taattcacag
     1321 ttacatacta aaggaaaagg gcaaggcgca aggctttgcc ctttcttctt ttttacatcc
     1381 aaaaacatat tgtttatgca actacaggct attcacaaac atatatatag ccatgaacta
     1441 agaaaggatg tgcagttatg catacattta gcttgccctt tggcaaagaa aaaatcaaat
     1501 tcgaactgcc ggaagaacag gtcgccggcg tcctcgtctc ccacgcccat gaatacaagg
     1561 cgccgaaatc ggaagcggaa ctcgtcgccg atgccctggc caacccgatt ggcagtccga
     1621 aattaagcga attggcaaag gggaagaaga actgcgtcat tatttccagt gaccataccc
     1681 gcccggtacc gagccacatc atcatgcccc agctcctggc agaactgcgc aaggggaacc
     1741 cggatatcga cattaccatc ctcatcgcta cgggcatgca ccgggctacg actaaggaag
     1801 aattaattgc caagtatggc aaagaaattg cggaacatga aaaattcgtc atccatgtca
     1861 gccgcaacga cgaagacatg gtcagcgttg gtactttgcc ctctggtggt gactgccgta
     1921 tcaacaaagt agccgctaat gcggacttgc tcatttccga aggcttcatc gaaccgcatt
     1981 tcttcgccgg catgtcgggt ggccgtaaat ccgtcctgcc tggtattgcc agcaaggtca
     2041 cggtcctggc caaccactgc tcggaattca tcaacagccc caacgcccgc acgggtatcc
     2101 tccagggcaa tcccatccat gaagatatgc tctatgcggc taaagcggct aaattagcct
     2161 tcatctgcaa cgtcgtcatc gatgcggaca agaaagtcat cgctgccttt gccggcgacc
     2221 gcgaaaaagc tcactatgcc ggcgttgact ttgaaatgaa actggctggc gtcaaacccg
     2281 tcccggctga catcgtcatc accaccaacg gcggctatcc cctggaccag aacatttacc
     2341 agtccgtcaa aggcatgacc gctgccgaag cgacctgcaa agaaggcggc gtcatcatcg
     2401 acgtatcgtc ctgctctgac ggtcatggcg gcgaagattt ctacaacaac ctgaagaatg
     2461 ccgaatccat ccagaaagcc atggacgaaa tcctggcccg cggccgcaac gaaacggtct
     2521 tcgaccagtg ggaagcccag atcctcatgc gtatgctcct gaaattcact atcatcatgg
     2581 tcactgaagc cccgcagcag atggtcgaag acatgcacat gaaatacgct gcctccatcg
     2641 acgacgccct ggctatggcc aaagacgtcc tggcgaaaaa aggcattacc gacccgaaaa
     2701 tcaccgtcat ccccgatggc gtatcggtca tcgtgaaata agaaccattt gtcgctcgta
     2761 atatggtgtt tgtaggggct cgcgcgtggc gnnnnnnnnn nccgccgggg atatgcgaga
     2821 tacttgtaaa cttgatttgt gtttttcttg ctatcccacc gtaattatca tataatgggg
     2881 gagatgtaaa tttgtcttaa cgtataaggg ggacttacct tggaagggtt agaatcgacg
     2941 cttgtctttg atatcactat gattttgcag gtcgtgctca tcgacttggc cttaggcggc
     3001 gataattcca tcgtcatcgg catggccgcc aagaatctgc ctaagcattt gcagaagcgg
     3061 gctattttat atggtacggc cggggccatc tgtatgcgtt tcatcatggc ctttatcgtg
     3121 tcctggctct tccagattcc cttcttgaag accgtcggag ccatcctgct ggtcctcatc
     3181 ggcatcaagc tcatcggcgc cgacgaccag gaagaagacg tccacgtcga agccaaggac
     3241 tcgctctggg cggctgtgcg gaccatcatc atggccgacg ccctcatgag cctcgataac
     3301 gtcctgggca tcgtcggcgt caccaataac cattggggcc tgctcatgtt cggcatgctc
     3361 atttccgtgc cgatcatcgt ctttggcagc accatcgtcg tcaaagtcat gaaccgcttt
     3421 cccatcctca tctacatcgg gggcgccatc ctcggctggg cttcgggcgg catgatggcg
     3481 acagaccatt acctggctca atacgtcagc ctggcggcca ataatccgac ggaactgaaa
     3541 atcatcctgc tggtcgttac cattgccggc ggattcattt ggaagcggat ccataaaaat
     3601 aaagaagttg tcgcctgaaa gttgtcgcct gacacgagaa aaaaagcttg actttttctt
     3661 ccagtatggt attatataca agtcaggtaa cggggcgtag cgcagtttgg tagcgcgctt
     3721 ggttcgggac taagaggtcg caggttcaaa tcctgtcgcc ccgaccatat ttttacacat
     3781 tggatgtcga tcccgagggc atcaaataac tgcataatca aacaatgtgt aagccatgga
     3841 gagctgtccg agtggctgaa ggagcatgat tggaaatcat gtatagggcg aacgctctat
     3901 cgtgggttca aatcccacgc tctccgccag aatgtaaaaa ggggatgtcg catatgcggc
     3961 atcccctttt tgagcattaa gtttgcagct tatagttttt agtgaatgat tttaaattta
     4021 aagccatttt tctgcaaact acaaactctg tactacaaat gctgtttttc aaggaggtct
     4081 tatgatggaa aaggtaagag aattggcggc acagtaccag caggccggga ataattgtgc
     4141 tgaaacggtg gtcaaggtct gcaatgattg tctggatttg aagctgccgc cggaaacgct
     4201 gcgcatggtg tccgtcctcg gcggcggggt ggccggttcg ggctgtcttt gcggggcttt
     4261 gaacggggcc tgtctggtcc tgggctgtct cgtcggccgc acgacgccgg aggaaaagac
     4321 gaaggccgaa gtcaatcagc ctgtgcggga attccagaaa atctggctcg accgctttca
     4381 tgcgacgtgc tgccgcgtcc tcaagagtcc ggccaacggc ggtcccgtat cgtgcggcga
     4441 cctcatggcc gatacgtcgg ccatgctcat cgatttcatc cgggaaaaga agctggcctg
     4501 atggggtgat tgaatttttt ccttgacttt tttcgtggat atggtattat gtatgagtcg
     4561 ggtcaacggg gcgtagcgca gtttggtagc gcgcttggtt cgggactaag aggtcgcagg
     4621 ttcaaatcct gtcgccccga ccatacttta catatcggca tttatcccga agatgtcagg
     4681 aaagatatgt aagacgcgga gagctgtccg agcggctgaa ggagcatgat tggaaatcat
     4741 gtatagggcg aacgctctat cgtgggtt
//
LOCUS       DAWABZ010000136         4742 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_4494_length_4742_cov_3.043738, whole genome shotgun sequence.
ACCESSION   DAWABZ010000136 DAWABZ010000000
VERSION     DAWABZ010000136.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4742)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4494_length_4742_cov_3.043738"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          917 a         1221 c         1420 g         1184 t
ORIGIN      
        1 gcgatgccca ggaggccgcc gatgacgccg atgacgacgg attcgatgag gaactgcatc
       61 atgatgttct ggaaggtcgc gcccagggct ttacggatgc cgatttcccg cgtccgttca
      121 gtgacggaga ccatcatgat gttcatgatg ccgatgccgc cgacgatgag cgaaatggcg
      181 gcgatactgc ccaggaagag ggtcagcatg gtcgtcgtgc tggtcatggt ttccatgagg
      241 ctcgtcaggt tgcggacgtt gaagtcgtct tccttgccgt tcatgatgtg atggcgctgg
      301 cgcagcagcg tttcgacttc ggactggacc tgttcgattt tatcggcgct ggagacctgg
      361 atgttgatgg agcggacgta ggtaatgccc atgaggcgtt cctgggccgt cgtcaagggg
      421 acgatgacga tatcgtcctg gtcctggccc atggaggact ggcccttgct ttccaggacg
      481 ccgatgatct tgtacggctg gttgttgatg cggatgttct tgccgaccgg gttggcttcg
      541 tcgaagaggt tggacgcgac cgtcggtccg atgacggcga cgcggttgcg cgagttgagg
      601 tcgctctgtg tgatgaagga gcccgactgg acgacgaggg agcggatgtg caggtattcc
      661 ggcgtgacgc cgtagaccga tgtattccag ttctggttgc cgttgacgat ctgataggaa
      721 ctgttgactg tcggcgatac gtagtcgatg ttctttatct tgttcttgat ggccgtcgcg
      781 tcgtcgtatt ttaatttctg gttcgacccg gctgccgagc ggacaccgcc ctgattggtc
      841 gagccgggca tgacgatgag catgttgctg cccaggctgg cgatggagtt ggtgacgttc
      901 tgcttgacgc ccatgccgat ggaaatcatg gcgatgacgg caccgacgcc gatgatgatg
      961 cccagcatgg tcaggataga gcgcagtttg ttggacaaca gggccgtgac ggcaatctgt
     1021 acgctttcac taaataacat ccatgacgac cccctttccg gcgtctttgg taatcatgcc
     1081 gtccgagagc aggagctggc ggttggcaca ggcggcgatt tccggttcgt gcgtgaccag
     1141 gatgatcgtc ttgtgctctt cgtggagtgc ctcgaagagg cgcatgatat ctttcgtcga
     1201 tttcgtgtcc aagttgcctg tcggttcgtc ggccatgatg atatgcgggt tgttgaccag
     1261 agcccgggcg atggcgacgc gctggcgctg accgccggac agttcgttgg gcttgtggtg
     1321 ggcccacgtt tccagaccga cggatttcag cttttccatg gcccgttctg cccgttcctt
     1381 gcggccgacg ccggcgtaga ccaagggcag ggcgacgttg tccagggcgg aaatgcggga
     1441 taagaggttg aagttctgga agacgaagcc gatgtgctgg ttgcggacga cggccagttc
     1501 atcgtcgctg aggtgggcga cttccttgcc gccgagcttg tacgagcccg acgtgggccg
     1561 gtccaggcag cccaggatgt tcatgagcgt cgatttcccg gagcccgacg gccccatgag
     1621 ggcggcgaat tcgccttctt ctatgttcaa ggtaatgccg gccagggcgg cgaaatcttc
     1681 accgccgatg tggtatatct ttttgatatc tttcaattcg atgacatgct tcatggcgat
     1741 gcttccttta catgggcggc ggtccctggc gccggttgtt gttcgatgac gaagatttgc
     1801 tggaggccgt ataggtgatg gaaaccttgt cgccctgggt caggcctgaa aggatttcca
     1861 cgtattcatc gctgtagatg cccgtcgtga ccgggacctg ttcggtctgg ccgttggcat
     1921 cgacgcggat gacataggaa ccggaatcgt tggtcttcaa ggcggcgatg gggacgacga
     1981 gggcatcgtc tttttgcgat gtcgtaatgt cgacgcgggc tgtcatgcct agtttcagga
     2041 cgtcgtccgg gtcatcgacg tcgagggtgg cgtaatagta gatgacgctg gccgaggagc
     2101 ttgaagagga gctcgatgtc gaactcgtgc cattcgtgtc ccacgtgttg ctcgtatcgg
     2161 tcttggcgat tttcgagacg tgggccgtga aagtccggtc cgtaaaggcg tcgaccgtga
     2221 aggtagcatc ctgaccgact tgaatcttgc cgatatccgt ttcatcgact ttggccagaa
     2281 tctgcttggt cgacgtgtcg gcaatgcgca tgatgactgt cgggttgctg ttgccctgga
     2341 cggccatggt gccgaccgcc ttcggttcgc cgacgacgac gccgtccata ggtgctgtga
     2401 tgaccgtttc agcgacatct gattcggctt ctgtcaaggt gctgacggcc gtatcataat
     2461 tgaacttggc tgtatccaaa gcctgtttcg tgccggcacc gagatcataa agctgctggg
     2521 cccggtcgta ttcgactttg gtattcgtga ctttatattg agcctggtcg cgcttggctt
     2581 catagtcctt gccgtcgagg atagccacgg tctggccggc tgtgacccgg tcattttctt
     2641 tgaccaggac ctggcggatg cgggccgtga ttttcggtga gacttctacg gaatcgacag
     2701 ggctgatggt ccccgtcgcc gaaaccgtcg agcgcaggtc tttgcggacg acgtcggccg
     2761 tttcgatggc cttggcctgc ttcgccgttt gttgggactg gtaatatgta tagccgccaa
     2821 aggcacaggc agccaggatg acagcggcaa tgacgatttt caggcgcttg tcaattttca
     2881 tggtatcact ccaaacgtat aaataaaaaa agattataag attattatag ttattattgt
     2941 tgttgttagc taagtatagc atgagcctaa aaaggaataa agctaaatac cctttttctt
     3001 agtaaagtga gttgaaattg tgacaaagtg actgtcctgc atagggtttt cccggcagat
     3061 gtgctatact aatagagcta atgatttata tgcagaaagg ttggtatcat attgtgaatg
     3121 aacgtaagac atctgtcttt tctaacggcc tgttatggtt cggcgccggc atttccatcg
     3181 ctgaaatcct gacgggcatg cttctggcac cgctgggctt tgccaaggcc tttgcggcca
     3241 tcatcctggg ccacctcatc ggcgggacag tcatgtatct ggccggcctt atcggcgggc
     3301 ggaccggcgg cagcgccatg gaaacggtgc gcatttcttt cggcactcag ggtgccaagc
     3361 tctttgccgg gctgaatgtc atccagctcg tcggctggac ggccgtcatg gtcgccagtg
     3421 ccgccttggc agccaatacc atcgccgcca ttggcgtgtc ctggtggagc ctcatcatcg
     3481 gcgccttcat tgccttgtgg atcgtcatgg gcctgaagaa cctgagcaag gtcaacgtcg
     3541 tcgtcatggc ggcgctgttc atcttgacca tcgtcctcag cctggtcgtc ttcaaaggcg
     3601 gtacgcccca ggtaccggct ggcgacctga cctttggcgg cgctgtcgaa ctggccgtcg
     3661 ccatgccgct gtcgtggctg ccgatcattt cggactacac gcggacggct gaaaagccgg
     3721 gcctggctac ggcggccagc tgtagtgcct atttcatcgc cagctcgtgg atgttcctca
     3781 tcggcatggg cgccgccctg ttcaccgggc aggacgatat tgcggccatc atggtccagg
     3841 ccggcatggg cgtggccggg atcctcgtcg tcctcttgtc gacagtgacg acgaccttcc
     3901 tcgatgcctt ttcagccggc gtcagctgcc agagcctggt tcccaaggtt tcggataagg
     3961 tcctggctct catcgccacc gtcctgggcg tcatcctggc catcttcgcc gcgccggacc
     4021 agttccagga cttcctctac ctcatcggtt cggtctttgc gccgatgacg gccatcctct
     4081 tgtcggattt cttcatcctc aagcaggacc acacggcaga cagcgtcaac tggctcaacc
     4141 tggtcctctg ggtcctgggc gtcatcttgt accgctttct gctcaacgtc gatacgccag
     4201 tgggcattac ggtccctgtc gtcgtcatcc tcatggtcat cagtgctttg gtccataagg
     4261 tattgaataa agcataaaga gattctaata ttgaattgat gaaatgcaaa aatgattcgt
     4321 aattagcatt gacaaaaagg cattgaaaag cgtaaactta tgctcaagca agataataat
     4381 gattgaagtc ttaagaggag gcttttcaga tgaataaaca ctttactttc gatgatatga
     4441 tcaataccat gatgcccctg cccatcagcg gcaacgccaa cggcgaaggc atctactgcc
     4501 gcggtcagga actcgtcgct tccatggacc agccgaaaga aacggcccat caggaaaaag
     4561 aaaaccgcgg attcgacctc cgcggcatct tctgccagta ttgcatcgat taataaaaag
     4621 ctagtggtta gtggctagtg gctagtggtt agcggatggt tttaaattta aggggatttg
     4681 ctgcaaacta aaaactacaa acttaaaact caaaaagagg ctgtcttcat gcgacagcct
     4741 ct
//
LOCUS       DAWABZ010000137         4668 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_4574_length_4668_cov_3.731194, whole genome shotgun sequence.
ACCESSION   DAWABZ010000137 DAWABZ010000000
VERSION     DAWABZ010000137.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4574_length_4668_cov_3.731194"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1119 a         1187 c         1161 g         1201 t
ORIGIN      
        1 ctgctggtcg gcacctgacg ggccaaaaag gcttagcagg acgtccccta cgatatcgca
       61 tacaggcggt ggaaatgaac tcaagacagc tgccggaacg gacaactttt gagataaaag
      121 cgtccggcat gatggcagaa atcctcacat agtattgact agtcaatata acagtgttat
      181 gatagattcc gtcaacaaat ttgactagtc aattataaaa ggagcgattg cagtgataaa
      241 agaaagatta tggactcgtg atttcatggc tctggtcctg gccaacggtt ttttttttgc
      301 cgggtttcat ttcctcttgc cgacgattac cctctatgcg gccagtttag gggctacggg
      361 tggggaaatc ggcctcatcg gcggtatctt tggttattcg gccatgtgta tccgtctgtt
      421 cactgatacg ggtgtccact atttagggaa aaagaagtgc ctttacctgg gcctggccct
      481 ttccattttg gctaccgtgt cgtattatat gtttgatagt atccacgcca tcgtagctgc
      541 ccgcatcctc cacggcttcg gttttggcct gagtacgacc tttgcggcag ctatcgtcgc
      601 cgatgtcatc ccggccagcc gccgcggcga agggattggt tatttcggcc tgggcagtac
      661 ggtcatgatg gccctggctc cggctgttgg tttattttta ctcaatgaca tcagcccagc
      721 agccctgttc tttgcctcgg ccgccgtttt cttcctggct gtccttgcca gtgtcttctg
      781 tcatgccggc aagaaagttc gtcgggtaga agtgaaggaa aaggcttctc tgcgcgaccg
      841 catctgtgaa accggtacgg gccttcccgc tgttttgacg attttcttcg gtgccgccta
      901 tggcagtgtc aatacgtata tcgccatgat ggctgctgaa gcgcatatcg aaaatgccgg
      961 cctgttcttc atcatcggga cgctgttcgt cttcattttc cgtccttttg gcggccgtct
     1021 cctcgatgct aaaggtgctt ttgccgtcgt catgcccgga tcggtcttgt acttgatcgg
     1081 cttattgctc atcatggcag ctcattcgct cacggtctta ttggcagcgt ccgtctttta
     1141 tggcttaggt gcaggcctgc tgcttccggc cctgctgacg tggattttga atatggtccg
     1201 cccggaacgg cgcagttctg ccagcgcgac cttctataat atgcttgata tcggcaccag
     1261 cacgggtatc ctcgtcttag gcgtcatcgc cggcatggta ggctaccgcc acatgtactg
     1321 gagcgttaca gctctcatga tttgctttgc tgtcctgttc atgttctgtc attatcgtta
     1381 tggcagcgat gtcatggcca gccagaaagc ggggaaagga gctgtcgccg atatccatga
     1441 agattgagag gtattacaat tatggatata caagaccggc aagatgatga actagtgcgg
     1501 cagttatttt gcacgacccg tatcttttcc cggactttaa atggctgtgt agcgcctttg
     1561 ggacttcaca gctcggaatg gatggtcctg aacttcatcc atttgaacga cggcattgcc
     1621 caggctgagc tgatccgcta tttcaaggtg gaagcggctg cgattacgcg cactttgagc
     1681 cggctggaaa agaaaggcct cattagccgc catcctatgg aaaaggggcg gggaaagtat
     1741 atcgaattga cagcatccgg ccaggacttg ttcgcccagg cagaaccctt tgtgacagct
     1801 catcggcgac gggctctgca aggcctgtca ccagaggaac gaaaactatt attgagccta
     1861 tgtgctaaag tacaagcggc cttagacgaa aaatagtata tgtttttatc atatagaggg
     1921 tatacctata ctgatttccc atagatagct tacaaattgc caatatatgc gtataattaa
     1981 atcaaaatat tacctatgct gaaaggagat tttacaaatg aaaattgtct tgttggaatc
     2041 gttaggtatc agccaggaat tacttgattc gtatgtgaaa cacttgaaag aaaaaggcta
     2101 tgaattcgct gcgtatgaac gcaatgatga cccggccgtc cagattgaag aagccaagga
     2161 cgccgatatc ctcatcatcg ccaatatgcc cctcaaagcc gaagtcatca atgcctgccc
     2221 gaacttgaag tacatcgacg tcgcctttac cggtgtcgac cacgtcgctc tcgatgcggc
     2281 caaagccaaa ggcatcaaag tcagcaatgc gtccggctat tcgacagttg ccgtagctga
     2341 attgaccatc gccatgatgc tcgatttgct ccgccgcgta cctcaggtcg atgcagcctg
     2401 ccgtgctggc ggtacgaaag ccggcctcgt cggcaacgaa ctggaaggca agaccgtcgc
     2461 cctcgtcggt accggtcata tcggccatcg cgtcgccgaa ctggtccatg cctttggcgc
     2521 caaggtcatc gcctataacg gcttctccca taaagccgat acggaactga ttaaatacct
     2581 gcccatgaaa gaaatgatgg aacaggccga tatcgtcagc ctccattgcc cggttacgga
     2641 caaatcgaag ggcttgatta ataaagaaac cttgtcgtac atgaaaccga cggccttcct
     2701 catcaacgaa gcccgcggtc ctgtcgtcgt atcccaggat ttggctgatg cattgaacag
     2761 cggtaaaatc gccggtgccg gcatcgacgt ttttgaaaac gaaccgcctc tcgacaccaa
     2821 tcacccgctg ctccattcca agaacactat cgttacgccg cacgtcgctt tcgctacggc
     2881 agaatccatg gcgaaacgcg ccgtcatcgt ctttgacaac atcgacgcat acttagccgg
     2941 aaatcagaag aacgtcattt tataatgata tagggcttgc ctggtagcaa gcctgcaggc
     3001 cgcggttcgc agaccgcaat cgtaggggcc gcccaaaggg cggcccgctt ttttttggcc
     3061 attttcatgc ctttttatgc acaaaaatca tggattcaat ttttctattc tacttattca
     3121 aattataatg tcttaccttg cttaatggat actagtatgg tagtatgtaa ctgcgaaaag
     3181 aaataaaaga aacatcaacg aaaacaaata ttttataatt catttataat tggaggattg
     3241 ccttatgaga atgaccttcc gttggtacgg agaaaatgat gacaaaatta ccttgcagca
     3301 gatcgaccag attcctggta tggacggtat cgtcggcgct ttattcgaca ttcctgtcgg
     3361 cgaagtatgg ccagtagaac cgctgcttca catcaaaaag caggctgaag cgaacaacct
     3421 ggtcttcaag gatctggaaa gcatcaacgt ccatgaagac atcaaattgg gcctgcccag
     3481 ccgtgaccag tatattgaaa attacatcac gtccctgcgc aatgctgcca aagccggcat
     3541 ccagatggtc tgctacaatt tcatgcccgt cttcgactgg acccggaccg acctggccaa
     3601 acccctttac gacggctcga cggtcctggc ttatgactac catctcatcg ccggcaagac
     3661 gccgcaggac atggcccggg aaatcctcga caacagcaat ggcttcgtcc ttcccggctg
     3721 ggaaccggaa cggctgaaag aattgtcgca cctgtttgaa cagtatgaag gcatggatga
     3781 agacggcctg cgcaagaacc tggaatattt cctcaaagcc atcgttcctg tcgctgaaga
     3841 agtcggcatc aagatggcca ttcatcccga tgatccgccg atttccgtct ttggcctgcc
     3901 gcgcatcgtc aaaaacgaag acgacctcga caaaatcgca gccatggtcg acagtccgag
     3961 caacggcttc accatttgca ccggttccct cggctcgaac cgtcagaacg acattcccca
     4021 catcatccgc aagttcggga aaatgggccg cgtgaacttc ctgcatatcc gcaacatcca
     4081 ggttcatgaa ccgtgggtct tcaacgaagt cgctcataaa tcgcaaatcg gcaacctcga
     4141 tatgtacgaa atcgtcaaag ccgcttatga catcgggttc gacggcccta tccgtccgga
     4201 ccacggccgc atgatctggg gcgaagaagg ccgtccgggc tatggcctct acgaccgggc
     4261 ccttggtgct aactatatct gtggcttatg ggatgctatt aaccgcacgt gcgggcagtt
     4321 ataaaaagga ggaacttacg atgacggcag aaagatttaa aaatatgcgc tgggtaatta
     4381 ttggactatg ctttttagcg aatgcgatta actttattga tcgtgctaat ttagctattg
     4441 cagcacctca tattcgggct gaattggcct tagacccgac aacgatgggg gttattatga
     4501 gtgctttctt ttggacatat gctttatgtc aaatgccggc cgggtggttt attgataaag
     4561 tcggtgttcg cgtaagcttg gcttttgctg ttgcttggtg gtctatcttt accgcagtaa
     4621 cagggatggt tcatactttt ggtcatatgg ttgggtcgcg attattgc
//
LOCUS       DAWABZ010000138         4666 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_4576_length_4666_cov_3.284537, whole genome shotgun sequence.
ACCESSION   DAWABZ010000138 DAWABZ010000000
VERSION     DAWABZ010000138.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4576_length_4666_cov_3.284537"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2594..2619
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3587..3596
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          977 a         1198 c         1338 g         1117 t
ORIGIN      
        1 attcaggacc agcgggctct gcatgtgctt cttggccagg cctttgatct tatccgggac
       61 agtggccgag aagaagagga gctgccgccg tttcggcgtc aggccgatga gggtatcgac
      121 gtcttccagg aagccggcct ggagcatctg gtccgcttcg tcgacgatga cccggcggac
      181 gccgctgagg tcgagggaat ggcggcggca gtggtcgaga agccggcctg gtgtgccgat
      241 gatgacgtgc ggatggcggt gcagtttctg cagctgattc tcgatggtct tgccgccgat
      301 gaggctgagg acgtcgatgc ccagttccgg tgccagcgta tcggcgacgt cggcaatttg
      361 acgggccaat tcccgtgtcg gtgccatgac caggacctgt tcggcgtgga tatccgtctt
      421 gatctgctgc agcgacggca ggaggaaggc aaaggtcttg cccgtgccgg tctgggcctg
      481 ggccaggatg tcgcggccct tgaacagcgg cggaatggcc tgggcctgga tggccgtcgg
      541 ttcgtcgacg ccctgcttat ggaggagttc acaaatcttc ggcgtaatgc cgaggatttc
      601 aaaattcttt gtcatgtatg ttcctttctg tgcggactaa aatcacttgc tataatttct
      661 attgtacaca aaagccaggc cttgtgcaaa cttttttttc ttttagggat atggtatact
      721 aaagtgattt catttgcatt attttaggag gtcttgcaga aaatgagttg gtggcgcgat
      781 atcttgcggc aatgtgtctt catgtgtttc tttatcattc ccattcccat cggtgcctat
      841 acgatccaca gcggctccag tgccgtcgta gccatggtaa gttatgtctt tttatcgctg
      901 gtcatccccg tagcctacgt gggctgccgc gatgcgacgt ttggccccaa gggcgggcgt
      961 atccgccgct gggtcatggc cctcatgtgg ttcatcgtct ttgcggctat cggggccttt
     1021 acggccttca tgggtgtcta ttggaaggct gcgcctttct gggaatggcc gaccattggc
     1081 cgtgatatcg tattcatcgt cggcatgtac gcagaaatca gcctgaccat gctcctggct
     1141 tacgccatat cccagctcct gccgggccga aaggaagtag gttaatggac aacttacaaa
     1201 aacagacatg gcacgatcgg ctgctggccg tcgtctgggc gtcgtgcggc atcctgccgc
     1261 tgccctttgg tgcctttgct tcagataggc catatatcgc ctcagcgtcg attctggcct
     1321 tttttatctt atcctttgcc attcccctgt ggctgggcta tcgccgccgt cgtgcgggca
     1381 gggacggcga ttatgcgtca tccggggcga ctctgtatgg cggcgccgtc gtcctgctcg
     1441 tcgctgtcgg cctgctcaac ggcctgaccg gcctggactg ctggcattct tatcccgtcc
     1501 gggtcggatt attcgccctg tggatgttcc tgaccgtcgc catacagtat ctggcggcac
     1561 gggccgtcga tttctggaaa gggcgccggc ggaaatactg gtactcccag ttcatcgatc
     1621 ccatcgtcta cagcctgccc ctgccgtgtg ccgtgctggg catgttcctc ttcccagccg
     1681 tcagcgactc gtccgtgacg cagagcctgg tcgtcggcat catggccatc atgggcttct
     1741 gcttcatcgg catgagcatc ttcgtcattg ccaccttcgc cttctatttc ttcccgtata
     1801 agcgctatgg ttatcagggg aaaaagcggc tggtccacat tgccgccatc gtcgtcatgg
     1861 tcctcatgtg ggccctggtc cagcacatcc tcttcgacag ccagatacac gtctttacgt
     1921 acatcttcaa ggccatgccg atcctccaga acaatcccct ggtcttcgtc acgcccttcg
     1981 tcgtagccgc cctgaccatc atcggctgcg tagccatgcg caacgtcgtc ctggcggcct
     2041 ttgaggggaa gaggagtttg tagtttgtag aaattgtagg ggcttgccgt ggcaagcccg
     2101 cctcagaata tgaatgattt tccggttcaa ggagaacgtc gcctgatatc cagcgggtcg
     2161 cccacgtggc gacccctaca gtaaaaaagg cttgtcgcac ggcgacaagc ctttttttta
     2221 tttaatttcc acgatggtct gcttcatgat ggtgaagcgg atgacgtata ccagccatcc
     2281 caggaagacg gcgaaacagc cgctgaggaa ggtcgtggca tcggtggtgg tcaatgcata
     2341 tacgcaatag ataatggata cgcctgccag gaatatcgac gtatttctgt tacagacttc
     2401 attggggata tgttccttgg tgaggatcgt cgtcagggcc ccggcacaga gcatgtaggg
     2461 gatgacgttg gtgacgacgg ccaggttggc caggagttcg aactgggcgg ccagggtcag
     2521 gctgatggtc atgagggcca ggaggctctg catgcagagg atgcacaaca tgcctttgac
     2581 cgggacgtca gcgnnnnnnn nnnnnnnnnn nnnnnnnnna tcttggggaa caggccgcgc
     2641 tgggcgctgg tcttgaagac gcgggccagg gtgaactgcc aggacaagag ggaaccggca
     2701 caggagaaca ccatcaggcc catgacggct ttgccggccg tatcgccgag catgtacgag
     2761 aaggccaggc cgaagggagc gctcgatgcc aggatttccg cattggggac gatgcctgca
     2821 atgacgttcg tcgaggcgac gtagatgatg gcgacgccga gggtgctgac gaaagtggca
     2881 ataggaacgg atttcttcgg attttcaacg gcatccatgt tgactgctgc cgattcgagt
     2941 cccaagaagg accacaaggt cagggcgatg gaattgccga cggcatcgct gagtcccagg
     3001 ttattggggt tccacgaagc cagccaggta gccgggctga accaatgcca gccgatgatg
     3061 gaaatgccca ggaccgggat gatggcaccc cagatggtga tggcactgag cttgccggaa
     3121 taacgggagc cgctgaagtt gagggctgcc gcgatccaca ataaagcgat cgtatataaa
     3181 cacgtctgta acggcgtaaa ggttgtctca aggaaaccgg cgccgtagcc gaccgccgta
     3241 atggcgatag cgacgtttgc aattacgatg gaaacagagt aggcgtaatt agccataaaa
     3301 tgtcctacac gcccgaaagc gtattcggag taaccgccca taccacctgg ctttgtcgtg
     3361 aacatgccac atcgggcaaa agccatggcc agggctaaag agcctagtga ggtaataatc
     3421 caggaaataa tcgagatcgt accgacttcg gcgagcttgg tcggcagcat gatgataccg
     3481 gcgccaagca tgttggcagc agtgacaaag gtcaactgta ccacagacat tttacgacat
     3541 gtagttttca ttatcagtcc ttctttcgtt agtgatgcgt ataagcnnnn nnnnnngtat
     3601 aagcatggct tgaaattttt agcaaattaa ttctatcatt tttgccggga aaattaaata
     3661 ctttttgaaa aaacgttata tgtatttggg ccacttttca cgctctatat tccaaatggg
     3721 aatagagttt agtggttcgt gtttcgtggc tcgtagcact gtaggaatcg tacgtgggga
     3781 cccgttggga atttgaatga gtttcccagc aatctgaatg gaagattgca taatcctgaa
     3841 gcgggcgccc tacgtggttg cccctacaga aatttaaaaa tatcagctga ctgctaaaaa
     3901 aggcttgccg ccgaagcgac aagccttttt acacatgaga gatgaaatta gtcgatgttt
     3961 tctacgacgc aagccgtacc ctggccgcca ccgatgcaca gtgtagccaa accgtatttg
     4021 ccgccgactt ctttcaagcc atagagcaag gttacgagga tacgagcgcc cgatgcacca
     4081 atcggatggc cgagggcgat agcgccgcca tggatgttga ctttgtccat atcgaaaccg
     4141 agttcacggc agcagtatac agcctgagct gcgaatgctt cgttagcttc gacgaggtcg
     4201 aggtctttga cttccaggcc tgctttagcc aatgctttac gggaagaagg aacagggccg
     4261 gtacccatga tagcgggatc tacgccagca gaagcatagc ttacaatctt agccatcggt
     4321 ttgaggccga gttctttagc tttatcagcg gacataacta cgaaggcagc tgcgccgtcg
     4381 ttgatgccgg aagcgttacc agccgtaacg gtgccgcctt ttttgaaggc cggtttcaat
     4441 ttagcaaggc cttccagggt cgtgccttca cgcgggaatt cgtccgtgtc gatgacgacg
     4501 tcctgtttct tctgatgaac cgttaccgga acgatctggg atttgaatgc gccgctcttg
     4561 atagcttcgc aagctttgac ctgagaacgg acaccgagtg cgtcctggtc ttcacgagtg
     4621 atgccgaaac gttcagcaac gttttcagcc gtgatgccca tatgat
//
LOCUS       DAWABZ010000139         4653 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_4589_length_4653_cov_3.360157, whole genome shotgun sequence.
ACCESSION   DAWABZ010000139 DAWABZ010000000
VERSION     DAWABZ010000139.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4589_length_4653_cov_3.360157"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1098..1107
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3448..3457
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          946 a         1273 c         1285 g         1129 t
ORIGIN      
        1 ttgttcggca tgtttttcgg ggcggggaac cttattttcc cgccttcttt aggttttcag
       61 gcaggttcgt cgttcatgcc ggccgtcctg ggatttctcc tgtccggcat cggtatgccc
      121 gtcattgccg tcatcatggg gaccttcaac cccggcggtt tccgggcgga aatgaaccat
      181 aagatttcgc cgctcttttc gctggtcatc ctgacgctca tgtatctggc catcgggccc
      241 ctcatggcca tcccgcggac ggcggcgacg tccttttcta ttggtatcct gcccatcacg
      301 ggcgatggca tgctgccgct ggccatcttc acgacgctct atttcatctg cgcctggtgg
      361 ctgtcgatta cgccgtcgaa actcctggac cgcgtcggca aggtattgac gcctatcttt
      421 gctatcatga tcgtcctgct catcatcgtc ggcatggtcg cctatacgac gccgtctaca
      481 gcagctccta tggggaaata cgccgcttcg gctttcggca acggcttcgt cgaaggctat
      541 aacaccgtcg acaccctggc ctcctttgcc ttttgcatca tcgccctggg tgccatgaag
      601 cggatccact tcgcgtcgga aaaggaacac gttgccagcg tctggatggc cggtgctgcc
      661 gtcgccgtcc tcatgggcgg gctctatctg ggactggccc tcttgggcaa tcattttcct
      721 attccccagt cggtctatga agacgccagt gtcaatctcg gcgcctatgt gttatcccag
      781 acatcattcc agattttcgg gactgtcggc atggcctttt tggctgtcat ggtcacgctg
      841 acctgcttca cgacgaccgt cggcctcatc gccgccgttt cgaccttctt tgccgaagag
      901 ttcccagtcc tgtcgtataa gaaatacgtc actctcatct gcctggtcag cctggtcctg
      961 gccaatctcg gcctgaacca gatcatccag gtcacgctgc ccctcttgtt gttcgtctat
     1021 cccattgcca tttccgtcgt catgctgact atcatcaata aattcgtcgg cttgtcgaaa
     1081 ctgggcatgc gctgtacnnn nnnnnnnatc tcgacggggc ggctgccctc atccagggcc
     1141 tgcccttagg cgacagcggc ctgggctggc tcgtaccggt cctgatctgc ctggtcctgt
     1201 ccctggtcct gcccgggaaa atcagcggcg aaagtgcgtt agatgaaagt ttgtaatata
     1261 agcgttcata gtttgcagaa aaaaagaggc tgcctttgta cgacggcctc ttttcgtgta
     1321 taatgaaatt ataacgatat taagtatcaa atatagctaa atgagataca gggagggaaa
     1381 gttattatgg aaaatgctgt catagggatc cttatcccct ttgccgggac gtctttggga
     1441 gcggcctgcg tctatgccat gaaacaggaa ttggatcgtc aggtccaacg agggctgacc
     1501 ggttttgcgt cgggcgtcat gattgctgcc tctatttgga gcctcttgat accggctatg
     1561 gaagaagcgg cgccgttagg gaagttcgcc tttgtgccgg ctgtggccgg attctggctg
     1621 ggcatcttcc tgctcctggc cctggaccgg ctggtgccgc atctgcacat gtacagtgaa
     1681 aaagctgaag gaccgcacag ccacttgcag cggacgacga tgctcgtcct ggccgtgacg
     1741 ctgcacaaca tccccgaagg catggccgtc ggcgtcgtct atgccgggct tttagccggg
     1801 cagacgggca tcagcgccgc cggggccctg gccttgtctc tgggcatcgc cttgcagaat
     1861 ctgcccgaag gggccatcat ctccatgccc ctcaaggcag ccggcgtcgg aaagcacaaa
     1921 gcctttctcg gcggcgtcct gtcgggcatc gtcgaaccca tcggtgcggc cctgaccatt
     1981 gccatgacgg ccttcatcgt accggtcctg ccctatctcc tcagcctggc tgccggtgcc
     2041 atgtgctatg tcgtcgtcga agaactcatc ccggaaatgt cggccggcga acactctgac
     2101 atcggcgtcc tgtcttttgc cttgggattt accctgatga tggctttgga cgtggttttg
     2161 tcgtgagatt ttttcaggag gaaagggagg tattcaggtg aaagtacgca ttacggatga
     2221 actggcccag caaatcgtcg attcggccag ggctgttgtc ggctggaata tcaacttcat
     2281 cgaccgtcat gggcgtatca tggctagtac ggatagcgga cgtatcggaa cgtaccataa
     2341 agccggtcac gttgcggccc ggaccgggca ggtccagact gttcaggaag accggcttga
     2401 ggacggcgtc agccgagggg tcaattatcc tatcatcatg gatcatcagg tcctgggcgt
     2461 catcgggatt accgggaatc cggctgtcgt cggtcagtac ggtttcttgc tgacgaaaat
     2521 ttgtgaagtc ttcctcaagg aataacgcct gagtcaggag gccttcagcg aagaagcgca
     2581 tcgcagccgc caggtcatgg ccttgattta tcgcgatgaa gatacggtgc agcagctggc
     2641 tgaagatcag cctgaattgg cgggaagctg ctatacggcc gtcgtcttcc gttggcatga
     2701 tggtttccgg caggctggag cgtttcgcca gaaaatggcc ggcttctgtc agaagttggg
     2761 ggtttcctta tatacttatg tttatccgaa tactttcgtt gtcctcgttc ccagcagggg
     2821 ctatgcagaa tgggacagaa aactgcctca gtggaaagaa tggttctatc cggaaatcct
     2881 ggccggtatc gggacggaag aagctttcca ccgtatctac gaatcgtacc gttttgccaa
     2941 aatggctttg caggcagccg gggaccggca ggtcttttgc gtccacgccg atgatatgaa
     3001 attggccttt ttgctgcaaa gtctggatgg tgccgtgcgt cgccgctact gtcaggatat
     3061 ttgtcacctt ttgacggaca gcgatatcca tttactccag gcctattatc agcaggattt
     3121 gtccatccag gagacggcac ggattctctg catccacaag aatacccagc aataccggct
     3181 gaagcggatt gctgggaaga ccggcctcga cccacgggta ttctgtgatg ccgtgcccct
     3241 ttacattgcc actcttcctt ggtttagaaa ataaaaataa tgacaatata ttgtgtctca
     3301 taacatggaa agggatgacc tcttttgcta cactataggc atagagaaac acagtcttac
     3361 gaatttgcag atagaggaga gatcatcatg aaagtcgttg tagccattga ttccttgaaa
     3421 aatagcctga cttccgtcga agccggcnnn nnnnnnntcc gtaaagccta tggcggccat
     3481 cctgtccagg tcatcgtcaa acccttggcc gatggtggcg aaggaaccgt cgaagccctc
     3541 gtcgacggat tgaacggcca gctccgctat gtcggcgtcc aggggccttt aggtgaagct
     3601 gtttcctgtc cttacggcta tctgccgggg atggaaacgg ctgtcattga aatggctggt
     3661 gcagccgggc tgggattggt gccggaagaa cagcgtaatc ccctgcacac gacgacatat
     3721 ggcgtcggtc aggtcatggc gcaggctata gaagcgggta tccgccattt catcatcggc
     3781 ctgggcggca gctcgaccag tgactgcggc gtcggtatgc tccaggctct gggttatatt
     3841 ttccgagatg cccagggcca gcctgtcggt ttaggcggac aggaagtggg gcacattgtt
     3901 tctgtcgatg attcccaggt ctgccggctc tgaaagactg taccttcgat gtcgcctgtg
     3961 atgtgaccaa tccgttgtac ggaaattggg gcgccgccta tatcttcggg ccccagaaag
     4021 gtgcatcgcc gcaggatgtc aaagtcctgg acgatgccag ccggtctttt gccaccgtca
     4081 cccagactta taccggtcat gactatagcc agacagccgg tgccggtgca gccggcggta
     4141 tgggattcgc cttcctggct tatctgaacg gccgcctgcg ttcaggtatc cagattgtcc
     4201 tcgattccat ccgattggcc gatgccgtca aggatgccga ttacgtcatt accggcgaag
     4261 gccgcctcga cgcccagacg gctatgggca aggcaccgat tggtgtagcg aaactggcta
     4321 aacaatacgg tgccaaagtc atcgccctgg caggctgcac gaccgccgac gctgctgaat
     4381 gcaaccggca gggcattgat gctttctttt ctatcgtcga taaggccatg ccgctggaag
     4441 aagccatgaa taaagaaacg gccctgcgca atatgaccaa tacggcccag caggtcttca
     4501 acctcatccg cgccgtcagc tgaacgagaa tcatccgatt acattaaata gaagcaagga
     4561 gaatagatat gctgaaaaaa acaaagatcg tctgcacttt aggccccagt tccaataccc
     4621 cggaaattat cgaaaagatg attaaagccg gca
//
LOCUS       DAWABZ010000140         4644 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_4602_length_4644_cov_3.114840, whole genome shotgun sequence.
ACCESSION   DAWABZ010000140 DAWABZ010000000
VERSION     DAWABZ010000140.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4602_length_4644_cov_3.114840"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1149 a         1288 c         1177 g         1030 t
ORIGIN      
        1 tggctacggc ttcgacgact tcggcatgcg gaccggctgt ggccccgatc ttgatttcct
       61 tgtcgctgct gccggaactt tccattgaat catcaatgat accccctctt aagattgtgc
      121 ctaaatgata cgctttcagt atagttttgt caatggcctt cctggcctct tgacaaatcg
      181 cctatccatg ataaactaag aatcaagaca actttagacg acatttatcc agagtggtgg
      241 agggactggc cctatgaaac ccggcaaccg acgatgacgg tgctaattcc tgcggattat
      301 tccgagagat aaagaatcag caatcaacct ctttctcttt cggaaagagg tcttttttat
      361 ggataaaatg acggaacagg atagtctata ttattctata ttttgttagg aggaatcttc
      421 atgaaaaatc tcaagaaact ctcgcttctc gccgcttcgg ccctgctggc cgtcgctttc
      481 ctggctgccg gctgcggcag tgattccgct tccagcggct cgtcttccaa gaacacgacg
      541 gtcaccgtcg gcgctacgcc cgtaccgcac ggcgaaatcc tcaacgaaat caagccgctc
      601 ctggccaagg aaggcgtcga cttgaaagtc gtcgaattca cggattacgt caaaccgaac
      661 ctggccctga acgacaaaga actggacgcc aacttcttcc agcattcgcc gtacctcgat
      721 aaattcaaca gcgaacacgg cacgaaactg aaagccatcg ccaagatcca cgtcgaaccg
      781 atgggcattt attcccataa gatcaaggac ttgaaagacc tgccggacgg tgcgaaagtc
      841 gccatcccca atgacccgac caacggcggc cgcgccctcc tcatcttgca gagcgccggc
      901 ctcatcggcc tgaaagacgg cggtaacatt acgtcgaccg tacaggacgt cgaaaagaac
      961 gataagcacc tccagttcgt cgaactcgaa gccgctcaga ttccccgttc catcgatgac
     1021 gtggacatcg ctctgatcaa caccaacttc gctatggaag ccggccttaa tccgctgaaa
     1081 gacgccctct tcctggaacc gaaagattcg ccgtacgcca atgtcctcgt catccgtgaa
     1141 ggcgatgaaa accgtccgga aatccagaaa ctcgtcaaag ccctccagtc tccggaagtc
     1201 aagaaattca ttgaagacaa ataccagggc gcaatcctgc cgagcttcta ataaaagttg
     1261 gatgtttgac gtttgatatt gagggggaat tcatatacat gcatacgaat gagctttttg
     1321 ccaaaaaaac ggtcttttct tttgaagtct ttccaccgaa acgggacatt cccgtcgaaa
     1381 cggtctatcc gaccctggac gagctgagcc agctccatcc tgacttcatc agcgtcacct
     1441 gcggcgccgg cggcacgggc ggtaaccgta ccgtcgaagt cgctacgtac atcaagcagt
     1501 taggctgcga aacggcggcc catatgccct gcatctacct cacggaagaa gcagccatgc
     1561 agaacctgaa agaattgaaa gaagcgggca tcgaaaacat cctggccctg cgcggcgacg
     1621 aagtaccggg ccgcgaacgg gctcacgtct ttgaacatgc cagcgacctc gtagccttca
     1681 tcaaagaaaa ggaaccggac ttcaacgtca tcggcgcctg ctatcccgaa ggccataccg
     1741 aagccaagac cctggctgcc gacatccgca acttgaagac caaagtcgac gccggtgcca
     1801 gcgagctcat ttctcagcta ttcttcgaca acgccctttt ctaccgcttc ctcgaacgct
     1861 gtgaaatcgc cggcatcaac gtccccatcg aagccggcat catgcccgtc accaataaag
     1921 ctcaaatcga acgcatggtc accatgtgcg gcgccacgct ccccatcaaa ttccagcggg
     1981 ccattgccaa atacggtgac actcccgaat ccatgcgcga catcggcatc gcctatgctg
     2041 tcgaccagat tgccgacctc ctggccaacg gcgtagacgg catccacgtc tacaccatga
     2101 ataacccgta catcgccaaa aaaatcgccg aagccgtaaa gacattgtta tagaaatctt
     2161 tgtacatcac ctctatagta ggggcttgca cgtggcaagc ccgtcccaga acattagcga
     2221 gtctcaagtt cagggaatgc attgccaaat ctctagcggg tcgcccacgt ggcgacccct
     2281 acagtgaaaa aaagagaggc tgtcttaata cgacagtctc tctttttttc actctttcag
     2341 cacataccca taggcgtgga tgcgtccatc gtcttctttt tccagataga cgccctggat
     2401 ttccgaggcg aagcccggca attcattgag gccttcttcc agggccttga aatactggac
     2461 tgccgtatgg gaccagcgtt cgcccgggac gacgcagagg acgcccggcg ggtacggcag
     2521 agcgccttcg agggcgatgc ggccttcgat ttcatcgagg ggcaccagct caccctggcc
     2581 gcggatgaaa gcgtattgcg catcctgggc attcatggcg tattccggca agtattcccg
     2641 acggaagagt ttcttctgca ggtccttgac gttccgcgcc ttatagaaat catgcatttc
     2701 ctgacagagt tggcgaatcg tatagccggc gtagcggtcc ggatgtgagt tgtagacgcc
     2761 gaggaggact tcctgcatgg ggacatcgtg gtcgacgaag cgttcaaaac ggaccagctg
     2821 ggacacgagg aggcgcatct tattcttatt ttcagccggt gtcagcagga agaggatcga
     2881 attcaaatca catttttccg ggatgacgcc gttaccgcga aggtacgtcg ccaggatgtt
     2941 ggcatggata ccaaaatctt cgtactgtcc cgattcagcg tcgatgcccg gtgtcgtcaa
     3001 taacaatttg caggggtcga cgagatattg tcccggcccg tatccttcaa aagcatgcca
     3061 cttggcatcc ggttcaaagg taaaataggc caggtcgttg gccatgtcat ccgtattgcc
     3121 atcctgccag ggcttgccgt agaccgtcgg tgccacgaac ggccggatat agtggcagcg
     3181 gcgcaggata tccttgcggg cttcgatacc cattttcacg cagtccatcc acatcttgcg
     3241 gccagccgca ccggcatgga ttttggcgtt gacagcgata gtggcgaaca tgggataaaa
     3301 aggactcgtc gacgcattgg ccatgaaggc attgttgaag gtcttgtggt tgcagtagcg
     3361 gggctggtta tggatatggt tgtccttctt atggatctgc gaagcctgag aaaaaccagc
     3421 ctgctgctta tggaccgact ggacgaccat gatacccgga tcgtcttcat gcaaatcgag
     3481 gagcagcggc gagtagtcct tgagcatggg aatgaactgt tcatagccga cccaggccga
     3541 atcaaagagg atgtagtcgc agagatgacc gatgcggtcg acgacatagc gggcattgta
     3601 cagcgtcccg tcataagtgc cgagctggat gacagccaag cggaaaggcc gcttggcctt
     3661 ggctttttcc ggatcccgtt cggctgccag ctggcgcaga tacgtttcat cgaaacagtg
     3721 ggcgtcgatg ccgccgatga agccataggg attgcgggcc gtttccaaat agaccggcgt
     3781 cgccccggcc tggatgagag ccccgagagc gacggacttg tgattgttgc ggtcatagag
     3841 aaccaagtcg ccaggcgtca gcaaggcatt ggtgacgacc ttattggacg acgaagagcc
     3901 attgaggacg aaataggtct tatcggcatg gaagacttcg gctgcaaaat cctgcgcttc
     3961 caaagccggc ccttcgtgga tgagcaaatc accgagggtc acgtcggcat tacagatatc
     4021 ggattgaaaa atatgaggtc catagaaatc ataaagatag cgtcctgccg gaggcttctt
     4081 gaaaaacagg ccgtcctgat ggcccgggca gtcaaaaggc gtattctttt cactgacgta
     4141 atcgacgagg tcggcaaaaa acggcggcag cacagcatgt tcatagcggc tggccaggcg
     4201 ttcggccgtc gaaatacagc ggctcttatt gaccatttct tccgtaaaga cgacatcgac
     4261 cttgtcgata tactggtccg gcatcttctc gccgcccggc atgatgacca tgatgggaat
     4321 accgaatccc gtttcatcga tcttgtcgat gtaggcaaaa tcctggtccg aaataaccgc
     4381 tacggccaca tcggtgaaat ccgtctgctc aacaggcacg atttcccggt cgctgtcaac
     4441 gaaataatac tgtacatcaa agctgtaagc tatcttcaaa tgtttcatat gtcttctctc
     4501 cctatcctct tcctttgcat tgatatttta ataaatacag ccctattgta cgcaattccc
     4561 ccatgaatag caagaaaaat atttcgcaaa tagtacattg gggcctaaaa agccgttcat
     4621 cgtaggtcgt tcgtcgttca tcga
//
LOCUS       DAWABZ010000141         4634 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_4616_length_4634_cov_3.023149, whole genome shotgun sequence.
ACCESSION   DAWABZ010000141 DAWABZ010000000
VERSION     DAWABZ010000141.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4634)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4616_length_4634_cov_3.023149"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1038 a         1323 c         1215 g         1058 t
ORIGIN      
        1 cggtagcaca gaaagccgat aatttcggca gatagccgtg ggcataaatg gcgaccatgc
       61 tggacaaggc cagtgcccgc agccgccgtt ccggcgtgac accgagcgaa tcggcgacga
      121 ccgtcacggg ttctgtcgcg gcgatgccct gattgccgga accggaattg gtcatagccg
      181 ggaaagccgc cccgcccata cgggcatcgg aagcggcgac cgtgcggatc tggatgcgat
      241 gggacaaatc atcggacaat aagtctttat cgaccatctg ctgcaaggta tagccgatct
      301 tcaggccata tttccccgtc aggccctcct gcgacagctg gtcgttgagc cgttccgcct
      361 cctgcaggaa ggcgatgtcg gccagcggca cgtgctcggc aaaatcgacg atatccgcca
      421 gggtcagcgt atggagaaaa gcgattttcg ggtcttcccc gctccccgct tccgtccgct
      481 ggtccaagag gacctggcca tccttttcca cgaagacgat gttcgtatgg tcgtcggtga
      541 tgacgacgcg gacgcggtgg ttttcgccat agacgacggc ttcggcatag aggacatgag
      601 gcgtatcttc ccggacggac acagtgactt taccatcggc gacgtactgt ttgcccaagg
      661 cgacgacgtc tggcgtcaag gccttcagga cctgtaatcc cgctccggcg tccccgtcga
      721 gggcgccgac ggcagcggca atatagagcc ccggcgtccc cgtgcccggt acggtgacgc
      781 ccatgccgtt cttcatcaga ttcgccgaca cgcgggcatc gacgaagcgc acggcttcgc
      841 ccaattcccg ggccgccgtc gccgccgcat aagccagggc aatcggttcc gtacagcccg
      901 tcgccggcgt cacatcctgc ttgatgatgc cgatcatctt gttccacata gcatttttat
      961 ccataattat ccctgctttc tcctataaat aggtatcata acgtaaatcg tatcacgtat
     1021 ctttctaata tcatcatagc atgattccta tgagaaaaca atatggatat gaggcagttc
     1081 gcagaccgca gttggcactt cgccgctctt ttaggtcctt cgcatattgc aagaaagaaa
     1141 aaaagaagct gtcgcacgaa gccaaaatgg cttaatgcga cagccccttt ttctgtaggg
     1201 gcgccacgcg gggcgcccac tccaaaatat gtacgatttc ccagccagtt tgctagaaga
     1261 atcacccaaa tccctaacgg gccgcccacg ggcggcccct acaaactcca tactagtgga
     1321 tggagttaaa tttttagtct tcgccgatga tgcggacttc cggttccagg tggacaccgt
     1381 gagccttttc gacgcgtttc tggacttcgt ggatgacgtt gaggacatcc cgggccgatg
     1441 cgtgccccgt gttgacgacg aagccggcgt gtttcatcga gacctgggca tcgccgcagg
     1501 agaggccttt gaggcccgtc tggtcgatga gggtcccggc atagtatccc ggcggccgct
     1561 tgaaggtact gccggcgctg tgcatttcca aaggctgctt gctgcggcgg cggttcatga
     1621 ggtcatccat gcgggcctgg atttccttgg ggtcgccgct gcgcagggtc aattcgactt
     1681 cgacgacgaa ttccttggat ttctggaaac ggctctggcg ataagaaaag cccaggttcg
     1741 gtgcatcata cgtatggacg ccgcccgtac tgttgaccgt gcggacccgc gatacgacgt
     1801 ggctcatttc cccgtcgtag gccccggcgt tcatgaagac ggcaccgccc aaggtcccag
     1861 ggataccgca ggcgaattcg atgcctgtca ggctgttttc ctgggcgaac tgacagatgt
     1921 ccttcagcat gaaaccggca ctggccatga tcttccggtc gtggcaggcc aatactttct
     1981 tcatcgtgtt cagctggatg acgacgccgc ggataccccc gtcgcggacg aggacgttcg
     2041 agccgccgcc gaggacggtg accggcaggt ccagccgccg ggctgcccgg atggccagcg
     2101 acatttcctt gaccgattcc ggcaagatca ggacgtcagc cggaccgccg acggcaaagg
     2161 tcgtgtgttc gctcatcggt tcgtccagca ggatccgttc catgggaacg gagatactta
     2221 attcggagac gaaagtcgtc agtgcatatt tattgatcaa ggatgctcat tccttctgcc
     2281 attgcatttt gaataatctg gaacacttcg ccagccatgg actgccattt attataatgg
     2341 ttcaaatatt ccacagcggt ctgattgttt ttaatgaact ctgccatctg gttgagatgg
     2401 tcgatcttct tgcggatcgg tttgtccccc atagactggg cataggccag ctgggcctgg
     2461 agcatgagga aatcgcggac cagatgcttg gtcttttcgt ctttggccag ttcctgaccg
     2521 gcagcgacga aatccttata ttcctgacat tccttgatag ctgcctctaa ctcatgggct
     2581 ttatcataaa tttccacgtt ctacacctca tcatttctgt aatgctaaag attcttgata
     2641 ccgatgccgc tcttgcccat gcccgggaaa ccgagctggc gcatcgcttc atacgatacg
     2701 atagcgacgg tattcgacaa attcaggctg cgcgcctgtt cgaccatcgg tacgcggaag
     2761 cagcgttccg gattggcttt caggaggtct tccggaaggc ctttcgtttc cttcccgaat
     2821 acgagcatat catcgtattg gaattggata tcggcataac actgttccga tttcgtcgtc
     2881 aaataaaaaa agcgatgatc cttgtatttg tccaggactt cctgaaaatt ctcatggatc
     2941 tggacatcga cgagcgacca gtaatcgaga ccggcccgtt ttaaatgctt gtcatcaatg
     3001 gaaaagccca ggggcttgac caaatgcagc gtaatatgat tggctgcgca taaacgggcg
     3061 acattgcccg tattgccggg gatttccggc tctaccatta cgatatgcat acttatatca
     3121 ctctccaagg gatattgtac aggatttatg caggaagtac aagaggaaca ttgcgtttcc
     3181 gaccttttta tgatatactt ataaataata gttattattt aatagaaatg taaagtttct
     3241 gtttatttta gaaagaggtg tcaattatta tggataactt tgacaacatt gcccagtacc
     3301 ccatgtattt cgctcccggc tgtaagctgc tgcagctgca gccccagatg gtttccgacg
     3361 tctacgatta cctgcgtcag ctcttcggca gcaaaatcaa actgtataca cgctgctgcg
     3421 gcctcgacga tgccaaccag cacaacgacg aagccgtctt catcacgctc tgcgacacgt
     3481 gctacaagat ttacggcgaa acctacgcca acctgcacat gcgcgacttc tggaacgtct
     3541 atgacgaata caaggacgtc tatcccctgg gcgaaaaaga aggtgaactc cgcaagacct
     3601 tgaagaacac cttctgtggc tccctcccgc aggaacacgt caagagctgg ttcgaagaat
     3661 ggaagaaatg gagtttgaac agtacagaaa aagactaatt cgtctttaag gagcagaggc
     3721 tatcatgaaa ttcacttact acggtcattc cgcattcaca ctttccgatg acgtgacgac
     3781 gattcttttt gacccgttca ttacggacaa tccctggacc caggtctgtc cgacagacat
     3841 tgcctgccag tatattatcg tcacccactg ccacggcgac cactacggcg acaccgaagc
     3901 cattgccaaa gccaatgacg cgacggtcat cagcaccgcc gaagtagccg gcaaagcggg
     3961 cgaagcaggc tgtaaagccc atgccatgca tgtcggcggc agtgcggatt ttccctttgg
     4021 caaagtacgg ctgaccccgg ccttccacgg gtctggcatt gccggagggc tggcctgcgg
     4081 tgttatcgtc gaattcggcg gcaaacgcat ctactatgcc ggcgatacgg gcctcttctc
     4141 agacatgaaa atcctcgacc gtttcggaca aatcgactgc gctatcctgc ctatcggcga
     4201 caacttcact atgggcatgg atgacgccgc cctggccgcc ctctgggtcc agccgcgctt
     4261 cgtcatcccc gtccactaca agacctggcc gatcatcgaa gcagaccctc aggaatacaa
     4321 gaagctcacg gaaacgaaat acaacatccc cgtccaggtc gtcgacccgg ggactacgta
     4381 tgaattgtaa tcatgatcct tcctgatttc tacggacgtt tccgctgcaa agccagcgcc
     4441 tgccaacaca cgtgctgccg gggctgggac atcgacatcg acgacgtgac ggccgattac
     4501 taccagcagc tcgaaggccc cttaggcggg gccatccgcc aacatatcgc cgaaggaccg
     4561 gacggctggc atttctgcct cacggcggaa gggaactgtc cctttctccg cccagatggc
     4621 ctgtgccagc tcat
//
LOCUS       DAWABZ010000142         4322 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_4986_length_4322_cov_3.315444, whole genome shotgun sequence.
ACCESSION   DAWABZ010000142 DAWABZ010000000
VERSION     DAWABZ010000142.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4986_length_4322_cov_3.315444"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          937 a         1131 c         1233 g         1021 t
ORIGIN      
        1 cgtagtaagc aaggagagag gaggtcgatg atggaaggga agctttggca gaatttgatc
       61 gatgccggtt gttcggcggc ttttatcgaa cagtatgagg ccctgcccga ggaagagcag
      121 ttgtcgtgcc tgcagcgcca tcgccggtac ttgctcgatg ctattcatga caaacagctt
      181 cagctggacc ggctggatta tttcttatat gtgttacgaa aaagaggaga cgaaagaaaa
      241 tgactttaga agaaatcaag cagacgagcc cctttgccat gggcggagaa aacgtcgact
      301 atgcccagta ttttgatggc accagttatt tgaacatgtt gtccctaaaa caggtcgtcg
      361 tcggcaatgt cacttttgaa ccggcctgcc gcaatcattg gcacatccac catgccagcc
      421 agggcggcgg tcagatgctg ttagtcacgg ccggccgtgg ctggtatcaa gaatggggga
      481 aaccggccca ggccctcctc gccggtgatg tcgtccacat cccggccaac gtcaagcatt
      541 ggcacggtgc tgccaaggac agtgccttcc agcatctggc cattgaagtc gacgggactg
      601 atacgagtac ggaatggtgt gaagccgtag cggaggccga ttatgatgcc cttccgtaag
      661 cacttcttag cggccgggct catggtcctg gcgggaatcg gatttctccc cttttacagc
      721 ggctggccca aggcctgggc ggccttcggc tatgccaagg aagtctacgg ggagtaaatg
      781 tctctatttg aaataatcag gaggtaaaaa gcagatgatg aagaaagttt taatgcttgc
      841 cctgagcagt ctcatggtcc tgggcctggc tgcctgtggc aatacgaacg gcgccgatgc
      901 gtcttccagc gcctcaaaaa agacggatgc ggcggcccag gccccggcca agggcaatgg
      961 caagacgctg gtcgtctatt attcggctgg cggtcatacc aagaatgtag ccaattacat
     1021 tgccaaagcg accaacggcg acgttatgga actgaaaccg gcccaggact atacggaagc
     1081 ggatctggac tacaataacc gggacagccg cgtctatcgc gaacacgatg atgccagcct
     1141 gcgcgacatc cctctggtca aggataaggc agacaactgg gaaggctacg atacggtctt
     1201 catcggatat cctatctggt gggggatcgc tgcctggccg gtagatactt tcgtcaaaca
     1261 caatgacttc agtggcaaga cggtcatccc ctttgccacg tcggccagct ccggcctcgg
     1321 cgacagcggc agacagctgc agcagatggc tggtacgggc aactggctgg aaggtaaacg
     1381 cttcccgtcc aatgtgtcgg aaagtacggg ccagcagtgg gtccagagcc tcaatctata
     1441 ggttccagcg catccttttc ctgctttaaa agctggatga attgctgcgt ccctttgggg
     1501 aacagctgaa aacgcttcca ggcgatgagc agcctgtccg aaaggggcgg gtccaggagg
     1561 cggaaacaga gggtatcatc gtcttccggg acacggatga tcttttccag catgatgcag
     1621 gaccccagcc cttcgcggac catgagggac gcattatagg ccaggttgta cgtggcgctg
     1681 atatgcaggt cagcgaaggg ctttttcagc cagactcgca gcctgtctgc ctgattgtca
     1741 tcctgcagga cctgattgga tacgatgagt ttttcatccc acaggtcctg cggggtgatt
     1801 ttttcttttt gcgagagcgg gttatcgcgg cgcaggagga ggccccagcg gtcgacagtt
     1861 gggcagggga ggacttcgta tttatccata tcgacgtggc catagacgac agccatgtcg
     1921 atgaggctct tgtcgaggag ttccatcatc tgctgaccat cgccgctggt gatatggaaa
     1981 tgcaggtggg gatacctggc ctggatgtca tgggcggcgt gcatgaggaa gcggacgctg
     2041 tccgattcgg ccgagccgat gcggatggtc ccgttcagtt cggtgtcgtc gctgcgcagt
     2101 tcctcttccg tatgactggc cagggagagg atttcttcag cccgcttttt caggaataag
     2161 ccttcttcgg tgagcgtaat gcgccggggg ccgcgaatca tcagttcttt tcccagcatc
     2221 tcttccatgt cgcgcagctg ccgcgacagc gtcggctggg ataggtgcag tgcctcggca
     2281 gcatgggtga tactttcttc gcgggcgacg gtgaggaaat aattgagtac ccgtaaatct
     2341 aacatccgaa tcagtccttt ccttgccaat ggatttcttc tttcaggccc ttcaaaaagg
     2401 cttcgcacgc cggtgacagg agttggtatt ttttccaggc cacgagacac gagaaggtga
     2461 cggctggttt cagggggacg aaggccaggt ccggcgtatg gagctggaag agctcgtcga
     2521 tgccgagggc actggtaatg cccgtccgga tgagctgggc cgcattggcg atgaggtcga
     2581 attttccgac gaccttcaag tcttcggccg ttacgcttcc ttgcgaccac tgacggatat
     2641 cgaaggttgc cgtagcagtc cgtgacgaga cgagcagggg aatctggctc agggtgtcgg
     2701 ccgtgatggc tggcagccgg gcccagggcg tatcgctgcg ggtgatgatg ccgacctggt
     2761 tgctttcagg cagacgcagg aattcgtatt tttccggatt gaaaggctcg atgaagaggg
     2821 cgaagtcgat gaggccgcgg gagagtttct cttcgaccgt atcggcgtta cccgtaaaga
     2881 gttcacacgt aatgcccgga tgtttccgct gcagtttggc aaaggtgtcg gttaggaagt
     2941 gcatgactgg tgtttcgccg gcaccgatgc ggatacagcc cgtcagttcc ggctggtcgt
     3001 cgctgatttc agctttcgtc tgctggacga gggacacgat ttcctcggcc cgctggcgca
     3061 ggcggatgcc ttcttccgtg agcagtgtac tgcggttggt ccggatgaaa agctgcttgc
     3121 ccaattcctt ttccagatcg gccatctgcc gggacaacgt cggctgtgtg atatgcagca
     3181 tattggctgc accggtcata ttctgttccc tggcgacggc caggaagtat tgcagtgtcc
     3241 gtatttccat gggtttcctc ctagggtatg gattttcttt agtatacgac ttttgaaagg
     3301 gccttacaac aaaggtttca tgcccaaaat gacatgaaac tgtaaccgtt gccatattgt
     3361 ggtagtacct tctccggagt ataataaatc attattattt tagatttatt gcttcggagg
     3421 aggctttttt tatggaatcc attgtgaata ctttttccta tgaaatggaa tatggcgggc
     3481 cgcacgttca tttcctagct tttttttatt catcttcatt ggcattgtca tcctgcacgg
     3541 cgtcttccgg atcgtcatcg aagtgctgag ccgcgtgacg aaaatcccgc gcgacagctg
     3601 gcgcaatatc ttccgtttca tgccgactct gatgggcatc cttttcatcc tcagttccta
     3661 tggcatttcc atttcgccgc tcctgacagc tctcggcgcc ggcggcctgg catcggccct
     3721 ggccttgcag gatacactgg ccaacctctt ttccggcatt acgacgctgg tatcgaaaca
     3781 ggtccacatg ggcgattaca tccgcctgtc cagcggcgaa gccggccgcg tcgtcgatat
     3841 gaactggcgt aatacgacga ttcgcacggc gacggggaat atggtcatcc tccccaacaa
     3901 gagcattgcc acggcgacgc tgaccaacta cgaacagcct ttggcagaat gtaccatttc
     3961 cattcccttg accattacct atgacaatga cctgcagcgt gtcgaagacg tcaccctcgg
     4021 cgtggcccgg cacatcctcg accacagtga atacggcgtc accggcttcg agcccctcgt
     4081 ccgctatgac cagatggggg aatacggcat ttccttcaaa gtcgtcctgc gtatccgcaa
     4141 catcgtcgac gaagccgtcc tcaagcacca gttcatcaaa agcatctata cgacctataa
     4201 aaaagaaaac atccagctcc tcatccgcca cgattgacat atatagtttg tagtacggag
     4261 tttgtagaag atgatttcaa atttttagcc atccgctaac cactaaccac tagccactaa
     4321 cc
//
LOCUS       DAWABZ010000143         4308 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_5007_length_4308_cov_3.184340, whole genome shotgun sequence.
ACCESSION   DAWABZ010000143 DAWABZ010000000
VERSION     DAWABZ010000143.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5007_length_4308_cov_3.184340"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          900 a         1184 c         1219 g         1005 t
ORIGIN      
        1 cctggctttt ttgcgccgat tcttgcgtga cataataatc ccccttattt attgacgatt
       61 ttcccgatat tcttcaattc aatggacaag gtcccatcgg ggttgttgat gaattggatg
      121 aagtcggtat tttcgatata ttccgacgga aaagtgatct cgatgcccgt atcggtcttg
      181 attttatggc tgcggccggc cttctgggca tattcctttt cgatggctac ggcttcaggc
      241 agtttcgctt ctttgacttg ttcttcaaat tcccgctgca ggaccggcga atcgccgaag
      301 acgtcgctgc ccaggtcctg cggcgagaag gaatcagccg tttcggcatt ttccaccaga
      361 taggtcttgg ctttggataa ggcttcgacc gtgctggcgc cgtgttcttc cgctactttt
      421 tcggcaatct tgctgacgac cttcacggct tccttgctgg aaatgaccga cgtgcactgc
      481 aggagcttgt ccggcaggag gtagacgtct tcaccgtcca ttttccgctt cttgtcgacg
      541 aaatggatag aaaaatcgtt caggcggatg agggcatagg cctcggcttt ctgcgccgtc
      601 ccgggcagga tggcgtaatg tttgatgagc ttgttgtaga ccgtcccttc gtcgtcgagg
      661 atctgatggg tataggccgt cttgttgtcc agcatgagga tgcccaggtt gcggacatcg
      721 tcgtcatcct ggaagtcgat gaccaggagg tctgtcgatt ccggttcatc acaatgggac
      781 acgagctggt agagctcgcg ggcgatctgg gacgccaaat cgtcgaaagt cagggtcccg
      841 tcgacgaggg cctgcaattt catctgaaag gcgctggtcg catagaagac gcccgtcttc
      901 tgggcggcat cgccgatgat gcggttcagc cgcttgctga cgtattcgta gacgacatcg
      961 ctgctgaaat cgaggtcctt ctgggataca atgcagacgt tggtattgaa atcaaaaata
     1021 tggagtacag ctgtattgat catcataatg ggcattcatc ctttacgctt gttttcgtaa
     1081 ttcaaagaca ctggcctcgc gggaacagag gacgcggaaa ggcagccagt ggcctgtgcc
     1141 gcggttgaca tagccccggc agtccccttt ctggaacatg ccgcggaggt acttgaagcc
     1201 cagggccgtg accaagggcg caatgggcgc gaactgggcg ccgtgcgtat ggccgcacaa
     1261 agtcaggggg atgtgatggg caaagccttc gtcgataaag gccgaatgat gggccagcat
     1321 gacgacaaag gcatcttccg gaaccttagc cagcgtttcc cggacatact gttcccgttc
     1381 tttggcaaag gcttcatcac ccttggcaaa gctgtaatca gtggcggcga tataaaaaga
     1441 ccggccgtcg cggccattgc agcccctcag gccctggccg cagccgcggc tggcctggaa
     1501 ggtggaattg cgcagtaccc ggaccggcgt gtcatcgagc atggctgtaa tcgcatcgaa
     1561 actgcggtaa tactcatgat tgccatagca gaagtcgatg ccgtcaggga agcggctggc
     1621 attggctttg aggatggcct gggccttggg cagcagggcc agttcatcga tgaggtcgcc
     1681 tgtgatttcc aggcggttca cgccctgtgc cgctacggca tcgagggctt cctgcaatcg
     1741 ccggggactg aaaaagagcc ccatgtgcag gtcagagagc tggccgattt tatagccgtc
     1801 gagataatcg ggcaggttct tgtatttcaa gacatggtag gtcgtatcca aatcgcgttc
     1861 gcccaagaga ttgcccgctc cgctggtggc gaaagccgcc acggggatca gcgccgccag
     1921 gcctttgagg aaagaccggc gcgtcacgcc caacgagcgc cgttgacgcc gacgccctct
     1981 ccggcggcgc cggggccggc agaaaaagcg ggctatggcg acgacgacca ggcagggaaa
     2041 ggccaagaga caggccagca gccagatcag gccggccatg gccttgccgt catagagacc
     2101 ttcccagccc gacgcgatga ggccgggaaa gaggtcttca taatggtaga gaccggctgc
     2161 cgataagacg gccagcagca acgtccccag gagcagcagc cagcgatgac ccgtgcggac
     2221 gacataatcc cataacagcg ccgcgacgac gcagatacag gatgtaatga ccatgaagtt
     2281 aataaagaaa agacctttca tcaattatac catggcagtg atcttgccgt gcggtccggc
     2341 ccagggcaag gacgcgtagt ccttgcagtc gatccactgc caatcctcct cttctccgat
     2401 tttcccctgg gtgatgcagc cttcatagac cgtcatctgc caggtcttat gcgaaaagac
     2461 gctcttcagg gagcctacag gctggtcatc ggcagccagg gtcagtcctt tttgcgccaa
     2521 gagctgacgg actgcagcga tgccctcttt ccccttcccc agggcattgg gaaattccca
     2581 catcgacgcc aagaggccct tgggcggacg gcggtggacg agccactggt ccccatggcg
     2641 gacgacaacg acggtaattt cttcgacagg cgatttcttc ttgaccagcc ggatgggcag
     2701 atcacattct ttcccagccc ggtgagccag gcagagccct tgcaaaggac agtccggaca
     2761 gcggggatgc ttggggatac agaccatggc ccctaagtcc atgagggctt cgttaaaggt
     2821 ccctggcgct tcgtcgggaa gctcttcttc tacgagagcc gtgattttct tcttgaccgg
     2881 cgtcgacaag atattctctt caatatcgta aaggcgggca aagatgcgca ggacgttgcc
     2941 gtcgacagcg gcttccttct gaccgtaagc caggcttaaa atggcgccgg ctgtgtaatc
     3001 gccaacgcct ttcagggact gtacgtcttt tttattttcc ggcacatggc cgccgtattt
     3061 gacctgtact tcctggaccg cttcatggag gttgcgggcc cgcgagtaat accccaagcc
     3121 ctgccactgc cgcaggactt catcctggga agccgccgcc agggcgggaa tggtcgggaa
     3181 atggtccatc cagctgtcat aatagggacg gacggtttcg actttcgtct gctgcagcat
     3241 gatttccgac acccagacct tatacggatc ccgcggcgat tcgtcgcgcc agggcagatc
     3301 ccggccatgg gcggcaaacc agtcgaggag cagcgggctc caatgttctt tctttatatc
     3361 catataaata agcctctttc tttctacact atcaactact agtatagaaa tccagaggct
     3421 ttttgtcaaa cacgcgttct ctcaagtttc atcacgtttc cgaatggacg ccgtcgcgaa
     3481 gatgcaggtc ctctttctta ggctgcacgg ctttgcgccc tttctgatac ttttcatact
     3541 gctgcagcat tttccattgg cggaaatttt ctgccatgat cttttccatt cgccatacat
     3601 acataaagag gccccctccc aaaatacgat ataataaaaa gcaaaaagac aacgacatca
     3661 tggacgacat cgttgtcttt tctttgtctt cattatatcg ctgattttcc tcttgtcaag
     3721 gaccacgaac cactatatcc tgtgcaggtg tttcagggat aaatccagga tgggcgcgga
     3781 atgggtcagg gcgccgctgg agatgtaatc gacgccgata tcggccaggg cggcgatatt
     3841 ttctttggtt acgttgccgg aacattccgt ttcagcccgg ccgtcgatga gggctacagc
     3901 ttctttcatc atgtcgtggc tcatgttatc cagcatgatg atgtccgcac cggcttcgac
     3961 ggcttcgcgg accatgtcca gcgtttccgt ttcgacttcg atcttgcgga cgaaaggcgc
     4021 ataggctttg gccatggcaa tggccttggc cacgccgccg gcggcactga tgtggttgtc
     4081 cttgaggagg acgccgtccg ataagttgta gcgatgattc ttgccgccgc cgacggtgac
     4141 ggcgtatttt tcaaagaccc gcatgttcgg cgtcgtcttg cgcgtatcga cgagggtcat
     4201 ccccgtccct tccagcaggg ctgcgacctg gtgggtatag gtcgcaatgc cgcttatgcg
     4261 ctgcaaatag ttcaggccta cccgttcagc cgacaagatg gcccgcat
//
LOCUS       DAWABZ010000144         4250 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_5067_length_4250_cov_3.501788, whole genome shotgun sequence.
ACCESSION   DAWABZ010000144 DAWABZ010000000
VERSION     DAWABZ010000144.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5067_length_4250_cov_3.501788"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          962 a         1225 c         1044 g         1019 t
ORIGIN      
        1 tttgagtttt aagtttgtag tttttagttt gcagcaaatg gtaaaaaatt taaaatcagc
       61 ttctacaaac tataaagtaa aaggaacctc gcatgatggt agaagccttc gtgcaccagc
      121 ccttcttctt actcgtgaat cgtcatttta ccgatggcca tggcaatatg cgggtccatt
      181 tcgtcataga tggtactctt gcccgtatcc agcatgcgga tggccttcat gtcttcctca
      241 tcgagggtaa agtcgaagac atcgatgttt tcctgcatcc gcgctttatg gaccgtcttg
      301 gggatgacga cgatatcccg ctggagcagc cagcgcagca tgacctgacc gacggtcttg
      361 ccgtgtttgt cagcgatgtc cctcaaaagc tgcgtatgga agacatcgtt gttcccttca
      421 gcaaagggcg cccatgcttc ctgctggatg cccagttctt tcatgaggcc ttcttcagcc
      481 catttctggt cccatacgtt ggtttcaatc tgattgacag ccggcgtgat ggtattcatg
      541 ttggccaggt ctgccagccg ttcgttccag aaactggtaa cgccgatggc ccggatacgg
      601 ccttcctgat agagccgttc catggcccgc caggccccat agtaatcgcc atagggcttg
      661 tggatcaaat agagatccaa ataatccagg cctaattttt tcatcgacac gtcgaaggcc
      721 ttcagggtcg catcatagcc atggtcctgg acccataatt tcgtcgtgat gaataactct
      781 tcccggcgga tgccgctctg acgcacggca tcaccgacac cttcttcatt tctgtaagac
      841 gcagccgtat cgatgaggcg gtatcccgtt tccaaggctt cactgacggc tttttccgtt
      901 tcagccgatg ccgtctgata gacaccaaat cctaatttcg gcatgatgac gccattattc
      961 aaggttacaa attccatgat ttcctcctat ttctcttcaa agggcagatg catattggca
     1021 tagcgttctt ctaaatccgg tgttgccgtg tagtactggc gcgtcccgtt cagcccgttg
     1081 acaacggcca tttcttcatc agtcagggca aaatcgaaga tgtcgaaatt gctgcgcaga
     1141 tggtccggat tcttcgtgcc ggggatggtg atgaactgcc gctggacagc ccagcgcagg
     1201 ataatctgtg ccgtactctt gtgatacttg tcggccaatg ccgtaaagat tgggtcgcgg
     1261 agcagttccg ggtcgccatg ccccaatgga taccagccca tgagcttcgt accgtattca
     1321 gccaggcggg ccatgatatc atcgtagacg tggtacgggt gcgtttccag ctggatgaca
     1381 tggggcttga catcggcatg ggccaggagt ttttccagtt tgtcgccatg gaaattggaa
     1441 atccccaggg aacgggcctt gccgtcgcga taggcctgtt cgatagccgc atacccggcc
     1501 agataattgc ctgccggctg atggatgaac agcagatcga catagtcgac gcccagccgt
     1561 tccagggtct tatcgacagc tcctgccttt tcatacagcg tcggccacaa cttcgtcgaa
     1621 atgaaaatat cttcccgttt gaccagacct tcttccaaag cccggcgcaa tcccctgccg
     1681 acagcttctt cgttgacgta ggcattggcc gtgtcgatga ggcggtaacc catcttcaag
     1741 gcagcataaa cggatttctc cgtatcttcc gggccgatca tgaaggtccc caggcctaac
     1801 aacgggacct gcacgtcgtt gctcaatgtc gtatattcca tggtataatc tcctttcaga
     1861 ctctgtttat atgatatagt gtgtaatagg tatttcctgt ctacatctcg cattttacaa
     1921 cagctttcaa aagatgtaca atgccaacag gggaattcac tatccacttt ttatatactg
     1981 aagcagagaa aggagccact atgatagaaa cgaacctgct tgcacaattt gtcgccttcg
     2041 ccaaggccgg caccctgacc cgggcagccc aggacctgca cacgtctcag ccggcactga
     2101 cgcgctctat gaaaaaactg gaagaagagc tggaggtccc cttgttcgtg cgccacaaaa
     2161 accacctgga actgacacag accggccgtt atgctgtcta ttacgcccaa cgcgtcctcc
     2221 aggaaaacca ggatttctgc gaccgcatcc gcagctatga ccgcagcctg cataccctct
     2281 ccatcggcta ctgtgcaccg gtcccgcaaa tggtgctgac gcccttgctg aacgggattt
     2341 tccagggcat gaccttatcg gccgacatga aggacgatgt ctccttcccg gaaggcctgc
     2401 gccgccagac ctatcagctg gccgtcatgc atgcccggcc agacggcgac gagttcttcg
     2461 tcaagaaatg cggcagcgaa aaactttatc tctcggtccg cgcatcggac cccctggcct
     2521 tttatccgga agtccgcctg caggacctgg atggccgggc catcctgctc ttttctcata
     2581 tcggcttctg gatgaaaatg acccgggaaa agacaccgca cgcccgctat ttgctgcaag
     2641 tccagcggga tgcattccat gagctgacgg agcactcgga ctacccctgc ttttcgtcca
     2701 gctatttcct cggacaggga caaaaaatcc cgggccgcat caacatctcc ctggcggacc
     2761 cggaatgtca gacagactat tacctagtct gcctgaaaag tgaacagaaa cgttatgaaa
     2821 aactcttcca gcaaatcgac gaccggacta tctactgatg ggattccagg tcccggcgca
     2881 gtttatccag gacctgggcc gtcaaatcgt gccaggcatc ctggagccac tggtcccgct
     2941 gcgcttcgtc gccgatacat tccagggcct tggggatggc ctcgccgaag aggatatctt
     3001 ccgtctgacg gctgcggtag acgccgagct tacaggcatt ggcaaagtgt tcacagttat
     3061 ggaagagcag gtcgtatttt ttattgccca ggcaggcacg ggcaaaggca atcgtctcgg
     3121 gtacagaaca gagctgtgcc tgttcttccg gcgtatactg acgcacttct acgtcaccgc
     3181 cctggcggaa ctcatcgaac gacgtcgacc ggatttccgt atcccaccag tcgatgggat
     3241 tgtagtcgtc atctccggca aaggcgatga cttcatcgtc actgacatag atgccgtgat
     3301 ggtaatacaa gcctcccagc cggcgcacgc ggatatggtc gcccataatc ggtgcctgcc
     3361 gggcccaatg ttccgacgcc ccgtcgtcct gcaataagga taaaatcgaa ttcaaatcaa
     3421 tggccatcgt cgtcactcct tacaaaattg cctaactctt accaggctgt caccattctt
     3481 ttacataatg ccgttatagt aataactgta aacgaagtca gctatccccg gcggcaaccc
     3541 accggcccgt taccgaaccc ctctgccagg atggcctccc gaccagaatc ggagaggttc
     3601 gccgtactgt ccaccggcca gcgtgccgct aaacttggta tcatagaatc agaaaaaacc
     3661 ccggagacct cccgctccgg ggctttttct ataccggcag cccctacagg aaggtcagca
     3721 gtccctgaca gccctcctgg acatcgtccc aggtccgctg gaaatcccgc gtataccacg
     3781 ggtcggctac aggacgggga ttttccgtat aatccatgag aagatggcac ttctggtccg
     3841 ggtcgccatg gaagatgcgc ttcatgtccc gcaggttctc gtcatccatg ccgatgaaat
     3901 aatcgtaaac tgcataatcg ccagccatca gcagcacggc ccgcttatca ctggggtcca
     3961 taccgtgtgc ccggagcagc cgttccactg gcggatagac cggattgcca tagccgttcc
     4021 agatttcctc atccgtcgta gccgccgatg ccacgtcgaa ccgttctgcc agccggcctg
     4081 ctcggccatc tgccggaaca caaactccgc catgggcgac cggcagatat tgccatggca
     4141 gataaatagt atcttgatca taaaggtgaa actccttcca tactgcaaat tacctgtaga
     4201 aaactattct tttttattgt aacagaaagc agtgattttt gccccgtttt
//
LOCUS       DAWABZ010000145         4217 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_5106_length_4217_cov_2.918068, whole genome shotgun sequence.
ACCESSION   DAWABZ010000145 DAWABZ010000000
VERSION     DAWABZ010000145.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5106_length_4217_cov_2.918068"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2544..2553
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          991 a         1188 c         1059 g          969 t
ORIGIN      
        1 accggcggac ttgatgtctt tgtttggcgt gaagaggtcc gggatcaaag aaatcttggt
       61 cttatcaaag acgggcttgc cgatcttgtt gaactgggca atggccggag gggccgtaat
      121 atcattggcc aggaccatat cgagattaca agtagttaaa tcacccggtt tcaccgagtc
      181 gacgccggca tgttttacca ggattttctg cgtcatcgtc atacccatat gtatatacac
      241 tccttattat ttcactaaaa tccaatacag ataagtttct atcatgcttg agttcgatgt
      301 gagtggcttt caattacact cccctcacat caaacatcat actttaatgc tggcccgacg
      361 tggctttgaa agccgtaatc gtatccggcg tggatttgag ggcccggaac ttgacgcgct
      421 gagccatgcc gtccatgcgg ttgatggccg tcatgaaggc gaagatggaa gccgtaatgg
      481 tatccgtatc gacgccggcg ccccaggtcg tatggccttc tttatcggtc aggccgaagt
      541 aagcgatgcc gcgggaatcg gagtcacggt ccaaatcgtg ttcgctgtag acgaggttgc
      601 tgaactcgat gccataggcc ttgcagacgg cattgctgac ggcattcagc tggccgttgc
      661 cggccccttc gaggacgact tcttcgtcgt tggcgcggac caggactttg ccgacccatt
      721 tatcgccttt cttcttgagc gagaagtctt cgactttcag cggcgtatcg gcattgaggt
      781 agttcttctg gaagacgtcg tagatttcgg ccggtttcaa ttcctgatgc ttgtggtccg
      841 atacttcctt gacgaagtag ctaaagtctt cgcgcatctt cttcggcatg tcgatgccgt
      901 aattctgttc catgacgaaa cctacgccgc ccttgccgga ctggctgttg atgcggatga
      961 cgtcggcatc gtacgtgcgg cccacatcat gcggatcgat gtacaggtac gggcaggtcc
     1021 agtactgttc gttgttttcc ttgcggaagt gcatgccctt ggcgatggcg tcctgatgag
     1081 aaccggagaa agcggcgaag accagctggc cggcataagg ctgacgcgga tggacatgca
     1141 tgcgggtcac tcgttcatac gtttccacca gtttgggcat gttcgagaaa tccaggcccg
     1201 ggtcgacgcc taaggcatac atgttcatgc cgatcgtgac gatatcggca ttgcccgtcc
     1261 gttcgccatt gccgaacagc gtaccttcga cgcggtcggc gccggcgagc atggccattt
     1321 cagcatcggc gacgcccgta ccgcggtcat tgtggggatg gaccgataag ataacgttgt
     1381 cgcggtattt cagattctgc gacatatagg cgatctgcga agcgaagaca tgaggcatgg
     1441 acatctggac cgtcgacggc aggttgatga tggccttgcg gtcagccgtc ggtttccaga
     1501 catcgaggac ggcgttgcag acttccagag cgtattccgg ttccgtgccg gtgaagcttt
     1561 ccggcgaata ttcaaagcgg aagttggcac cggcttcgtc ggtcaggcgc tgtaacaatt
     1621 tcgcaccgtc tacagcaatc tgtttgattt cttccttcga tttgcggaag acctgctggc
     1681 gctgggcgaa ggacgtggaa ttgtagacgt ggacgatggc gttcttgacg cctttcaggg
     1741 catcgaaagt cttgcggatg atgtgttccc gggcctgggt caggacctgg acggtgacgt
     1801 catcgggaat catgtcgtgg tcgactaagt agcgcaggaa ttcaaattcc gtttccgacg
     1861 cagccgggaa gccgacttcg atttccttga agcccatggc gaccaagact ttatagaatt
     1921 caatcttttc ttcaaggctc ataggaatga tgagggcctg gttgccatcg cgcaaatcga
     1981 cgctgcacca aatgggcgct ttatcgggat attctttctg ggcccaggac atatcgacaa
     2041 cagggggcat aaaataacct tttttataat gttctacgtt tttcatactg catctctcct
     2101 atgatcatga aataaattta aaataaatca ttacacaatc aaaataaaaa aaatctttca
     2161 tctcgaaaag agacgaaaga ttatacttac gcgttaccac tctaattcct atccgaagat
     2221 aggctctcaa caggtacgga catacgtcga taccctcctg tctgttaacg gccaggcccc
     2281 ggctccacct actcctttcg tttcagcgag ctgcttagag atgagttctg ttccgtgtcg
     2341 ctgctgcctc acaccatccg gcagctctct ggaaacattc cggaacttac tagttctctt
     2401 caacgcattt tttattttgg attgtttgtc tgttgacgtt ttgtatgtta tcataccctt
     2461 ttgtaatttg caaggctttt tccagaactt ttccaaatat ttttagctta aacgtgatac
     2521 tttatttaat aaaattccgt gaannnnnnn nnncgtttat ctttacatgt aacgattctt
     2581 tgcaggatgc gtcgcctccc ccctatcttt ttccccttat taatgctata ataaataagt
     2641 tacacaacaa ggaggaagta catgttagaa aaattccaaa atttgaatat tagcgaagtc
     2701 attatccgtg ccctgaatga aatgggcttt gaagaaccga caccgatcca ggctgaatcg
     2761 attcccatcg ccatgagcgg cagcgacatg atcggccagg cccagaccgg tacgggtaag
     2821 accgcagcct acggcatccc ggtcctggaa aaaatcctga aagccgaacc gtccaaggat
     2881 atccagaccg tcgtcctgtc gccgacgcgt gaactggcca tgcaggttgc cgaagaactg
     2941 aaccacctgg cccagttcac caacatccag gccctgccga tctacggcgg ccaggatatg
     3001 gaacgccagc tgcgccgcct gcgcaaatgc ccgcaaatca tcgtcgccac gccgggccgt
     3061 ctcatcgacc acatgaaacg cggcaccatc gatttgagcc acatcacgac catcgtcctc
     3121 gacgaagccg acgaaatgct ggacatgggc ttcatcgatg acatcaacct catcatggcc
     3181 gctacgccga atacgcgcca gaccctgctc ttctcggcta ccatgcccaa gccgatccag
     3241 cagctggcag aaacgttcct gcacgacccg ctcatcatcc gcatgaaggc caaagaagtc
     3301 acgatggacc tcatcgaaca gtcctacatc gaagtgccgg accgccagaa attcgacatc
     3361 ctctgccgtc tcctggacct gcaggaaccg gacctggcca tcatcttcgt ccgcaccaaa
     3421 cgccgcgtcg acgaagtgtc ggaagccctc aagaagcgcg gttactcggc tgaaggcatc
     3481 cacggcgacc tgacccaggc caagcgcgat acggtcatcc gccagttccg tgaaaagacc
     3541 atcgacatcc tcgtcgctac cgacgtcgcc gcccgcggcc tcgatatcag cggcgttacc
     3601 cacgtcttca actacgacct gccccaggac ccggaaagct acgtccaccg cgtcggccgt
     3661 accggccgtg ccggccagag cggtgaagcg acgaccttcg tcatcccccg cgaaatggaa
     3721 cacctgcgga ccatcgaacg cctaatcaag cgccgcatca cccgccgcaa ggccccgacc
     3781 ttctcggaag ccctggaagg cgcccagcag gccgccatcc gcagcctgat cacgacgaca
     3841 gaagaaggca acttcgggac gtataaagaa aatgccgaag aactcctgtc caactacgac
     3901 tcggccgctc tggtagccgc agccatcaag ctcttgacca aggaaccgga cacgacaccg
     3961 gtcaagatta ccgaagaagc gccgctccgc agccgcaaca accgcggcga ccgcaacaac
     4021 cgcggcgacc gcaataaccg ccgctacaac cgcaacgacc gccgtggaga ccgccgcaat
     4081 cgcaacaaca accagcgccg tgaaaacaaa caacataaag aagcgaatcg ttccgtattc
     4141 aagccttatt tcaaggattg agaaaagggg ctgtaacaaa atgagttttt ttgctcattt
     4201 tgttgcagcc ttccttc
//
LOCUS       DAWABZ010000146         4184 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_5152_length_4184_cov_2.652458, whole genome shotgun sequence.
ACCESSION   DAWABZ010000146 DAWABZ010000000
VERSION     DAWABZ010000146.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5152_length_4184_cov_2.652458"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          918 a         1138 c          971 g         1157 t
ORIGIN      
        1 accacatacg aacctcaccg tactggtatc acaggacttt tttacagtcc ggatccggcg
       61 ggaagtcgca taaaaataag ggcatagcac aagtaacagc tatgcccttt attatatcat
      121 caatcttcgg ttttatacac ccttggcggt ctcttctttt gcattctccg ccgttgcttt
      181 cgtcagcacg gtctttacca caaacaccag cccttccatg accagcggca gaacgatgcc
      241 atcacggacg cggcaccagc cggattcctg aggagcctgt gccttcacct tttcagaaaa
      301 gccatctacg gcattttcaa tgattggcat aacttgttcc agcagagtgt tggtgacctg
      361 gtctttcagt tcatcggtca cgacatcgac attcagcgca tcaacaatat tgtctctcac
      421 atcagtccat ttgctcatga tttaatctcc tttcgctgtc aaaaaaatag ccgatatttt
      481 ggcatgtcat tttttccatg tggcccgata ccagttagcc ttgccgcgca gcacgtcgcc
      541 gccacggctg ccgtccgtcg cccaaggatt aaaggacgga ctctcaggcg tgccaagata
      601 ttccaagtcc caccgctcac aggtggtcct aggcccgtac ggctcgtggc aggcaatccc
      661 gtcctcgttg tctgccgctt ccccatgtgt catgacatgg ggcttgtcga tggtaagcca
      721 cagtccgtcc gcaagggctg ccgcgacctg ggccagcgtc tcaatctgga cggccgttgg
      781 ggcgtagtcg cctaagtcgt cgctggtggc gttgtaggcg cagtcaaggg caacgccgat
      841 agctccggag ttccgccgcc acgtgtgggc cagcgtctcc gttaggtctg ccatcaacat
      901 gatcttgcca ttgccgtgga tacagacgtg gtagtcgtca aagtctgctt cgtaccgtcc
      961 tgcggtccag tgcaggtata ttttcgggtc acgtcccatc tgtctggcct gctcaaaaag
     1021 ttcgttccgg gctgcgtctg ccagctgctt cagctcgtca actgttacta tttttgccat
     1081 tagttccgtt ccctcctttg atggtcccgc cgatatagcc taagaggcca gtcacgatgg
     1141 acatggctaa gttgtccata tggctgagga cggccatgat gaggctcacc gagagggcca
     1201 ggatgaccaa aatattttca acgtcgattt tttcaaattc aaacatttgc taacgcctct
     1261 cactcatatg gagcacgtcc tcgtgcttgg cctgcatgac gccattttcc gcaaggcgct
     1321 catacacgcc gtacatctcc tgccagatga gcttttcctc gaccgtcggg ggcgcgcttt
     1381 gaaaacggca gtacatatcg ttgagggacg cccgcaggat gagctgcatc cccttacgca
     1441 cggccttgag gcccgtcaca taggccacac agtacccggc tagcccgccg atggcaagac
     1501 taatgagcgt gtgtgacccc tccatcaaaa gatcgctcat tctttgctcg ctccttcgtc
     1561 cttagctttc ttttcgccga cataagcgat acatctttcg ttgctgcagc tgccatcgga
     1621 ctttagctcg ctcccacagt actgacaata ttttttccgt ttccaaaaac tcattttacc
     1681 gtcactcctt ccaccgcttc tttgtaagcc gctgtcatag ctttataatc ttgctggata
     1741 ctggacactg cgtcggaatt tccggcaagt gtagctgctt gcagtgcggt cagcatctcc
     1801 gtcttgttag cctcgtactc agcggcaaga gatgcctttt ctgcggcagc cttttcttct
     1861 gcggtcggtt ccggcgggac gtagtcaacg ggacggcccg tcttgctgtc tcggaccttg
     1921 ccgtcgaggt aggcgataaa gtcgtcggac gtgatgatgt cgaccaccgc agcgcttgct
     1981 gcacaagctt tgacctcgtc caatagggcc gcccgctgtt tgggattttt tagcgggtta
     2041 aagtcacaga ttttggttgc tacacgcttt cccgtggcgt caaacgccgc ggcgtagtaa
     2101 gatacattag ttgctgtcat gttttttacc ctctttcgtg aaaggatgat gaaaaaatga
     2161 gaaaacccaa tgggtatggg agtatcaagc atctatcggg ccgcagacga cggccctttg
     2221 tttttgtagt gagtgaagct ggacgtcagc gaccggttga atattttgtg acccaaacag
     2281 aggctgagat atttgccgct gattacccaa acgccgctca tatcagggtt tgaagattca
     2341 agagtgattt tgatgccgga ttgctgaagc tgttgtattt tgacggaacg aaagctatct
     2401 gcgattgtag atgaaccaac cagcgtggcc attgaagcaa ggcaatccat tttaaagcta
     2461 actggcaaag caacctgcca ttgctgcttt gtggactccg ttcttatcca ctgtttaagc
     2521 gcctacggca taccaatcag catctttggc ggtgccgtcg ccgacgacgg ttaaggtaaa
     2581 ccgagttaaa gaaacggcgc ttggctttgt ttgataaaaa tctatcccct catgctgggt
     2641 taaaatgcga gggatgagca aattgaaagc gactgggtag taaacatcac cttgatatcc
     2701 atgtccccac tgtataatta ggtttccaaa aaatgaacca aaacagatgt acccgttttg
     2761 agcggcaagg tatctgaccc cgctagctgt cagcaccatc ttgagcagct ggccgaacca
     2821 gctgtttgtt tcgagcgctt tgattgctgt gatggtcgtc gtgagggcca gctttttgat
     2881 gatgtcatca ccgagtgttg cgattttctc aaagagctta aaccggtttt cgtgagcagc
     2941 gtctgattcg tcgtgagcgg caaggtctgt ccgagtcgtg agtcccttcg ggtcaatgat
     3001 agccattacg ctatcagcat tagtgatgac cacttgcaca tgcaatccga cagacagtgt
     3061 agtgctgctt cctttcgcct gcaggtaatc agcatgacta tcggtggcta cggcgtacaa
     3121 tatttcccct tcttccggat ccgtggcata tatgccaagt tcccgcatat aaaatccttt
     3181 ttctagctcc gcattggtga taacaccttc aatatcgcaa ataccagcat cagtgggcga
     3241 aatggatgaa atgccaatat caagtttggg cgcagctaaa tctgtcatgc tttcaatttc
     3301 atgcggttcg ccgtctccca ccttcagctt tgtcagctcc aacttacaga tgcctgcggt
     3361 tacttttgct tccagggctc ttccctgttt tgtcataatt gctccagacc aatcagccat
     3421 tttttcctcc tatccagatt tccttaaaaa gaaatgcagc cgtcgcaaat cgattcggct
     3481 gcaatacatc cacattatta atttttggta agccgatggt tatctctttt gacacaccgg
     3541 cggctatcgc atagcgtttt cttaccttta gttctctttg ccatcttaat tcgtcaagcc
     3601 agctgcgaac atttttggca ttttcaattg cacgagtaaa agcttccaca cttcccccgc
     3661 atcgcacctt atcgggcatc tgttccacgc ggaagtgata cgggtttccc ccgtattcat
     3721 accattctct gaccttagct cctccagcta gagcctccac gactacctta accgcataca
     3781 ctgtgccttt atatcgatgc acggctatcg ccgtcttgac ttgcttacgc ttaatgtcaa
     3841 tgtccgcaga atcatcataa tcctcgacgt gaaattgcca tgctaaaagg tccaaaaggt
     3901 tttcaggcag ctgatcgata cggctataga ctaggacatt tgggatgaga gttgtaacag
     3961 cagtcaactc gttagaaact gcgtccgctg tttttctaac gttctcgtct tccgagatag
     4021 aagtgggcaa gatgcttttc aattcaacgt cttttagctt actcattcat ctgccccccc
     4081 taaaaatgtc accgttacgt cttctgccac tgccagttca gttcgctgca gctgcttgat
     4141 gactggagcc gtcaccgtaa cctttcgagc tccagcttgt acca
//
LOCUS       DAWABZ010000147         4178 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_5164_length_4178_cov_2.455008, whole genome shotgun sequence.
ACCESSION   DAWABZ010000147 DAWABZ010000000
VERSION     DAWABZ010000147.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5164_length_4178_cov_2.455008"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3121..3130
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          989 a         1198 c         1095 g          886 t
ORIGIN      
        1 ccattactct ccccactttt cttgacggta tagttgctgt ctagctgata ctcttcttat
       61 tatacatgaa agtcaccgta tgtatgtata ttttttgtaa aaattttcgg catgcctctc
      121 tattctgtag tataatggaa aaggtaaacg ataaacttga taaacatccg cagaaacgag
      181 gtataccatg gaaaaattag ccatcatcga catgggctct aactccatcc gttttgtcgt
      241 catgcagatt gccgacaacc agtcttattc cctgctctat caggaaaaag aagccatccg
      301 cctgggccac ggcctgagcc agaccggcgt catcagtgaa gaagggatgc agcgggccct
      361 gacctgcctg catgtctata agcacatcat ggaagtcatg gacatccatg aatgccgggc
      421 cgtcgcgacg gcagccgtcc gcaatgccag caacggcgaa gctttcctga agcgcatcaa
      481 tgccgaaacg ggcatcacca tgaacgtcat caccggtgaa cgggaagcgt acttaggcta
      541 tctcggcgtc atcaacacca tcgccatgaa ggacttcctc atcttcgacc tgggcggtgc
      601 cagcgtcgaa atgaccttag tccgcgacgg agaagccgtc cattccctgt cggtccccat
      661 cggcgccgtc acactgacgg aaaaattcgg cacccagggc aatcctgatt ccgaagccat
      721 tgccagcctg atgaaattcg tccgcaaaaa gatggccgcc gtcccctgga tagaggacat
      781 ccagctgccc atcgtcggca tcggcggtac agcccgtaac ttcgccaaaa tggaccagcg
      841 ggcgacgaat tatgaactgt cgaagctcca taattacatc atgcccctgg aacattttga
      901 aaatctctat catgaaatca cgacgcggac cagtgccaac cgcaagaaaa tcgacggcct
      961 gtccagtgaa cgctctgacc tcatcgtcgc cggtgcagcc gtcatcaaga ccatcttcga
     1021 catgacgggc agcccggaaa tggtcgtcag cggctgtggc ctccgcgaag gtttattctt
     1081 tgaatactac gcttcgtact gccagctgcc gtcaccgcgt ttcgacgaca tcctcgattt
     1141 cagcgtcaag aacttcatcg gtacgctgag cggcatcatc atcaaccagg ctcactacga
     1201 ccaggtcacg cgcattgcca cccagctctt cgaccagctc cagccgctcc acggctacgg
     1261 cccgcgggaa cgcctcctgc tgcagacagc cgcccgcatg cacgatgtcg gcaagatcat
     1321 caacttctac aaccacgccc gccattcggc cttcatgatc gctcatgccc cgctctacgg
     1381 cctgacccag gtcgaacaaa tcattacggg cttcatcgcc ggcttccatc acggcatcag
     1441 ccgcaagatc atccgggcct accgttacgc caacatggcc tctgccgaag actggatcat
     1501 gatccgcaaa ctctcgacca tcctggccct ggccgaagcc tcggacctga cgtatgaaca
     1561 aatcgtcacc gacgtcgaag tcacactggc cgaaaacgtc gccgtcatga tcatcacgac
     1621 ggcgccgggc tcgacgtaca acgccgctga ctacgaaatg aaacaactga gcaaacaatt
     1681 caaaaaagaa ttcggcgctt ccctgctcct cgtctggaag taagggtccg gataaaaaaa
     1741 ggggctgtcg cacatgcgac agcccctttt tactcttatc gccgcatcat gtcttcagcc
     1801 gtgacgtcgg gatggtggta ctggtcttca gagaggatct gtttaccatg gacggtgacg
     1861 gcttgctggg gacaccagtg gacacaggcc aggcaatggc tgcaatgaga gccgatgacg
     1921 gcccctttcg ccgtacagat gatattgctc accggacaga tgcgggcaca gaggccgcag
     1981 gcaacgcagc gtcccccatc gacagctggc gtcagccagc gttccccagc atggcggatc
     2041 aggcccaggt tcatgagcca ggccgacagc gacgaatgga tggccgtcgt atcccggacc
     2101 tgcccgcgta tcttatcggc tgcggcagcg accagttcat cggcgtggtc caggcggttc
     2161 atatcgtagc ggatatggcg ccgcgacgcg atagtactgt tggcgatcat cggcaggaca
     2221 gccccgtagg acaaagaaca atgttgacgc cggaggatcc ggtccatcgt cctgagcgac
     2281 cggccaggcg cactgccgca agtagcaatg ccataaaaat aggtcgattt ctggaaacgg
     2341 acctgtctga tgaaatcctg tatgggttcg ggaagatccc cgaaatagac gggaaagaca
     2401 aagccgatga tgcgatcctg gatacgctgg caccgttcat ggatagccga aatcggcacg
     2461 gccgtatcac cagtcagttc agccagcttc ttggccacat ataatgaatt tcccgttcct
     2521 gaaaagtaaa gaatcataag gctcacctca gagaccgcac tgccagtttc ccgcttgcag
     2581 gcggacggta ttttacatcc attatatcat gaaaccctgt catttgtctt gatacatttg
     2641 tctttacacg taaccgcgct cgcaaaggct gacgaaggtc ccgtcgccga tgatgatgtg
     2701 gtcgaggacg ggtatttcca tgatctggcc ggcgtcggca atgcgctggg tcacgacgat
     2761 atcctcgtgg ctcggttcgg ggttgcctga cggatggtta tggaccagga cgacagctgc
     2821 ggcattgtag cgcagggcat agcggaagac ttcccgcgac ttggccaggc tggcattgat
     2881 cgtgccgatg gtcagcgtgc gataggcgat gagctggttc ttcgtcgata aatagacggc
     2941 gccgaactgc tcttgcggca aaaagcgcag atcttccatg acatagccgg caatggcctg
     3001 gggcgtactg aagtcaggcg ccgcttcctt tacgtgctgc cgggaaatac gccgtcccag
     3061 ctcgacggcg gcacagaccg tgacggcctt atccgtcccg attccgggaa tctgacagag
     3121 nnnnnnnnnn ccgatgctgg tatcactgag ataacaaata ttttcccctg gcagggaatg
     3181 aaggacctgc cgcgacacat cgaggacgga ttgtcccttc gtacccgtcc gcaatagaat
     3241 cgccagcaaa tcctggtcgg tcacagccga aggaccgtga cggaggaatt tttcccgcgg
     3301 tttctcacaa acagcgaggc tgcggaaagg gtcgctcata catggacctt ggctttgtgg
     3361 aacaacttgc atacctggga caagggcagg ccgacgacgt tggtatagga accattgatc
     3421 ttatcgacga aagccgcagc ccggccctgt atgccatagg caccggcttt gtccagcggt
     3481 tccccagaag cgacataggc tttgatttcc ttttcactca gtcgtcggaa ccagactttc
     3541 gtttcgacga ccttcgtgta ttccttatgg ccattgatca aagcgatgcc cgtatagacg
     3601 gagtgtttct ttccggacaa ttcccgtagc atctcgacgg cagcggcttc attgcggggc
     3661 ttgcccagca tttccccatc gatggcgaca atcgtatcgg cgccgaggac ccactgtccc
     3721 gggaattttt cagctacatc gcgggccttg cccaacgcct gggcctggac gtatttttcc
     3781 ggattctttt tcttcggtga ttcttcactg tacgtactcg gattgaccat ataggcgacg
     3841 ccgatctgcg tcagcagttc gcggcgacga ggcgaaccag aagctaagat gagcatgtaa
     3901 taatccctcc taaattattg aagatgacga aagagaatgg ctgccaggat gacgccgatg
     3961 atgctgagca tattcggtgc cagatggatg ccaaactgca attccatgag atagagattg
     4021 aggtgaatat tctggatatt caagatttca taatgctgcg tcagggccgg caggataccc
     4081 ttcaaggccg gaacagcata cagggcctct ccgacgatgc caccgaggat accgccgacg
     4141 atgaggaaaa taaagaagaa caacagactg tgtgagcc
//
LOCUS       DAWABZ010000148         4175 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_5168_length_4175_cov_3.055583, whole genome shotgun sequence.
ACCESSION   DAWABZ010000148 DAWABZ010000000
VERSION     DAWABZ010000148.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5168_length_4175_cov_3.055583"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          836 a         1125 c         1112 g         1102 t
ORIGIN      
        1 ccgtattctt ttgcggcttc gtatactcgt ttcgtcatct agttaccccc ttctattcat
       61 cacgcttcac tttctctatg gccccggcga agcctttgtc atcaatgagg atgatgacgc
      121 tctgggcttt gccgacggca ctgcctaatt catttttcgt atatgtatca acgacggtga
      181 tccctttccg ctccgccagg cggcgatatt tctcgacgtt atcgctgccg ccgtcggagg
      241 ccaggaataa gagcgggacg cgccggcgct tcaatgtttt ctgtgcggct ccatcaccgc
      301 agacggtctt gccagcccgg caggcaaggc cgaggagatt caggaccttg tcgctcacag
      361 cagttccttc ctcagctgtt catagatttc gctggacact ttggtcttca gggaccgttc
      421 caggcgatgt tccttgtagg ctttttccag gcattcgctg ttcagacaga gatagacgcc
      481 gcggccggac ttcttgccgg tcttgtcgat ctcgacagcg ccttccggcg tgcggacgac
      541 gcggatcatt tccttcttgc tcttctgttc ctgacagccg gcacagacgc gcatgggagt
      601 ctttcgggtc ttcatggctt attctccttc cgtatcggct gtaccttcgt cagctgccgg
      661 catggccagg tcgctgagga tgtcgatgtc atcggccggt tcactgtcct gcggagcttc
      721 tgcctgggca gccagttcgg cagcctggct ttcgctcttg atgtcgatct tccagttggt
      781 cagtttcgca gccaggcggg cattctggcc ggccttgccg atggccaggg acagctggta
      841 atccggtacg acgacgctcg acgatttttc gtcttcatcg accgtgaccg aaatgacctg
      901 agccgggctg agggcgttgg cgatgtagac cgccgggtct tcatcccatt tgacgatgtc
      961 gatcttttcg ttctgcaatt ccgagacgat gttctggacg cgcgagcctt tcgggccgac
     1021 gcaggcaccg acggaatcga cattggcatc gcgggaatag acggcaatct tagaacggcg
     1081 gcccggttcg cgggcgacgg atttcagttc gacgacgcct tcgtagattt ccggtacttc
     1141 cagttcaaag aggcgcttca agaggcccgg atgggtccgg gatacgagga tctgggcgcc
     1201 tttcgtcgtc ttgcggactt cgacgacata gcacttgatg cgctggccga cgctgtacgt
     1261 ttccgacggg atctgttccg atgccggcag gatggcttca gccttgccca ggtcgacgta
     1321 gacgttgcgg ttctcgatgc gctggatgat gcccgtgatg atgtcgtccg tgcggtccga
     1381 gaattcatca tagacgatgt tgcgttccgc ttcgcgcagg cgctggatca tgacctgttt
     1441 ggcagcctgg gcggcgatgc ggccgaaatt gtccggcgtg acatcgacgt tgacgacgtc
     1501 gccgacttcg taattgacat caatgaggcg ggccttatca agagagattt ccgtatgcgg
     1561 gtcttcgact tcgtcgacga cggtcttggc agccttgatg gcatagtcgc ccgtcgagcg
     1621 gttcagttcg acttcagccg tcggattggc atcgggttcc ttcttgtaag ccgtgatcag
     1681 gacggcttcc agggagtcac agatgacatc cggagatacg cctttctctt tggataagta
     1741 agcaatagca ttgagcaatt ctttattcac gttaataacc tccactaaaa atcaatatgc
     1801 aaacggactt gcgatatttc tttcatgtca atcttatctt cacggccatc gacctggacg
     1861 atgagatgcc cgtcagctgt cttttcgacg agatctgccg tcaggtcctt gacgccgtcg
     1921 cgggccttaa agaaatggat gtctacggac cggccagcat agcggacgaa gtccttgtcc
     1981 ttcttgagga tgcggtccag tcccggcgac gatacttcca ggagatagtt gtcgctgatc
     2041 gggtcctttt cgtcgaggac cttgctcagc ttctcgctga cggcctggca gtcttccagg
     2101 tcgacaccac cttctttctc aatgaatacg cgcaggtacc agttccgttc gcgcacgtat
     2161 tcgacgtcga ccagttccag gtcagtccct tcgataatct gttctacttc cttggcaatc
     2221 aaatcttcaa tgtgttcacg tctcatcgta ttcaccagct tatcttcaaa atttcctaca
     2281 ggtaaaagtt aagagtgggc tttcacccac tcttcgcatc taaacatcta aattactttt
     2341 agtataccac tgtctgctca ttctgacaat gatttgtccc ggtcagccgc gatttcgtcg
     2401 aggacgggca ggaggcgttt ccgcaaggct ttctccagct ccttcaaaga cgacgtatcg
     2461 aagtcgtgga ccaggtgcgg gaagttctgc acccgccgtt cagcaaagaa atcgtcgcgc
     2521 aggacgttgt acatcaggga caggccgctg cgcgtggctg cgtcgtaata agccaggatc
     2581 atgtaggacc cgttgatgat gcgcacggca tcgttgggct tgaccaggcg ccggaagccc
     2641 tgatagctgt cgggcaggaa ctcatcccat gcgacgtcag ggacgccctt gttcttgagg
     2701 atgacgctga aattgcggct ccgttcgacg taatcgtcgt ggatgatacg ggccaggcgc
     2761 tcttcgacca tggaccggaa gaggtcgaag gaatcggtga tgaattcgat gagggcgaat
     2821 tccagcatgc cgatatccgt ccggatagag aattcttccg attcgggatt caagatagcc
     2881 cgcacggtcc agccgttgac gtgattctca taggtgaaca agcggtactc gacgggcttg
     2941 ccgtctttct cgatcatgcg atattcttcc aataactgaa aatctcccac tgaaagtggg
     3001 agaacagttg tgatattcca ttgtttgagt gtttcaatca atgtagcatc catagtaaaa
     3061 atggtgcggt tggcgggagt tgaacccgca cgatcgaaga tcactgccgc ctgaagacag
     3121 cgcgtctgcc attccgccac aaccgcataa ctcagaaatc gtaaagctgt ttgtaatgct
     3181 tttcgttcag caagactatt ctaacatatt ctcgcgtttc tgaaaagggg attttttcaa
     3241 tttcttgaaa atttttcgtc cagccatatt tctggatcca cgagtcgaca tgtccccggc
     3301 cggcattata cgcagccaag gccagtactt gattcccgtc gtattccttc aataagtagg
     3361 ccagatacca ggttcccaac ctgatgtttg tacggacgtc attgagtttt tcaggcgtgt
     3421 agtcggtcat ccccatctca tcggcaatcc attgtgccgt gtcgggcatg acctgcatga
     3481 ggccatgggc accgggctgg gaagcagctc gttcgtcaaa cttgctttcc actaagatga
     3541 cggacgccac cagggcgggg tcgactttgt cttcgtaggc atactgccga accaggtaat
     3601 cgtactggag agggtatacg aacttccgca tgacatcatc tttccaagaa taaaagtacg
     3661 atgcggacag acatatcaat aatgccgcta tgatgatttt ccctttggtg cttgctcttt
     3721 ttctcaacgt cttctcccta ccctttgatg cggtccatca aggcctgcca tgcgacgagt
     3781 acctgtttct gcgtctcttc cggagagccg gtattgtcga tgatgacctg tgccaaagac
     3841 cgtttgtcgt cgataggaag ctgtgcatga atccgtgcca gtgcttcact ttcgggatag
     3901 ccgttccgct tcatcagccg cgtaagctgc gttaccggat cgacgtacac cagccacgtt
     3961 tcgtcaacgt acgaatcgta ttttacctca taaagcaggg gcatgtcaag gaaaatgaca
     4021 ggattttttt tattttcttc gatttttttg atttccccgt cgatgccggc atggatgagg
     4081 gggaataaaa tggcttcgag ctgcgctttc ttcgccttat cgccaaagac gacgctgccg
     4141 atgtaggccc ggtccatgga ttcgtcggct aagag
//
LOCUS       DAWABZ010000149         4160 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_5181_length_4160_cov_3.182460, whole genome shotgun sequence.
ACCESSION   DAWABZ010000149 DAWABZ010000000
VERSION     DAWABZ010000149.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5181_length_4160_cov_3.182460"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          987 a         1106 c         1157 g          910 t
ORIGIN      
        1 ctcagataac tgagtccatt tttttcgatg aggctattat gttacagccc cttttctatc
       61 ccctggaagc gagtataata aggtatcata taatcatgga gggcataacg atggatgaac
      121 gggaatggga acagttattg catatcaaaa cggctggccg cgacgacagc cattccgatg
      181 gggagcacca tccttatgag ccgacggatt acggcgtcct cgaacgattg gccaacagcg
      241 gctatattgg caagagggat acgctcatcg attacggcag cggcaaagga cgggtcagct
      301 tcttcctggc ttatcagacg cgctgtcagg ccatcggtgt ggaatacgac cggcggctgt
      361 gggaaaaagc cctggccaat ggacagacgg cggcgtcccg gcggcgcgtg actttcgtct
      421 tggccgatgc cgtggagtac gaggtcccgc cgcaggggaa tcgtttcttt ttcttcaacc
      481 cctttgccct gcacacgctg aagcgggtcc tggggaaact tttcgacgcc cattaccagg
      541 cgccgcggcc gatgcgcctg tttttctatt atccttatga agaaacagaa gctttcctga
      601 cgcatcatcc caacctcgtc gttgaagacg tcatcgactg ccgggacctg ttcgatgagg
      661 acgatgaccg ggaaaaaatc ctggtcctgc ggctgcgtga ctgattatcg gcaatttatc
      721 ttatgaaaat attcaagtta aacgattgat aaattgccga taacggcata aaattgaatt
      781 aacgattctt aaagaaggag aatgattact atgatagaca cggaacacgt tgtacagcgt
      841 tttttgaagt atgtttcttt tgatacccag tccgatgaag acaatgatgc cgtctgcccg
      901 agtacaccgg gacagctggt ctttgccaag gccttggcag aggaactgaa ggcaatcggc
      961 ttgtctgatg tcaccttgga tgaacacgga tatatcatgg ccacgctgcc ggccaacggg
     1021 gaagaaggcc cggtcgtcgg cttcatttct catgtcgata ccagtcccga tgcatcgggg
     1081 aaggacatca agcccgtccg ggtcactgat tatgacggca aggatgtcgt cctcaacgaa
     1141 gcaaggggca tcgtcttttc gacgaaggct tttccggaag tcctgaaata caaaggccag
     1201 gacatccttt ttacggatgg gacgaccctc ctcggggccg acgataaagc gggcatcacg
     1261 gctatcgtca gcgctatgga atacctcatc cagcacccgg acatcgctca cgggatgatc
     1321 cgcatcggct tcacgcctga tgaagaaacg gggcgcagtg cggctttgtt cgatgtgaag
     1381 cagtttgctg ccgactttgc ctatactgtc gacggcggcg aactgggcgg tctggaatat
     1441 gaaaacttca atgctgccaa cccggtcatc accttccatg gccgcagcgt ccatacaggc
     1501 gacgccaaag gaaagatggt caacgcattg tcgattgccg cagaatggca gcagctcctg
     1561 ccggccgggg aaaaaccgga atacaccgaa ggccatgaag gcttcttcca cgtctataaa
     1621 ctgaatggcg acgtcgaaac gtgcacgatg cacatgctcg tccgcgacca cgaccgccag
     1681 cgttttgaac ggcgcaaggc cctgctgcag cagatggccg atttcttcaa tgaaaaatac
     1741 ggcgatggga ccgttgtcgt cacgccgtat gatgtctatt ataatatgct ggaaaaaatc
     1801 gaagacggcc atatggatgt cgtcgacttg gccaaagaag ccatggaagc agtcggcgtc
     1861 acgccgcagg tcgctcctat ccgcggcggt acggacgggg cccagctctc tttccgcggc
     1921 ctgccgtgcc cgaacctgtt cactggcggc gccaacttcc acggccgcta tgaatacctg
     1981 cccattccgt cgctggtcaa ggcctgcgaa acggtcatcg aaatcgccaa gcgggcagct
     2041 aaacgtctgt aggcgcctga tggtcctgca acgatgaaca acctgtgaga agacaagacc
     2101 ttcgtagggg ccgtcccaaa gggcggcccg cctgatatga tggtgtatac gtaatgcata
     2161 acgggtgatg tgtgaaaaca ttcctcatat acctgaatag ttatatccaa gattcacagc
     2221 gggacgcctt agcaggacgt cccctacgga gcggttcagg cgatgtggcg attctcgtca
     2281 ttctcaggat ggggaatgat aaaaggggga attttgatga aagagattac tcatgatatg
     2341 ttagcccagt tccgcacggc ttatgaagcc gacgggcagg cccaggtcct tcattcggcc
     2401 ctggccaaga cggacatggc tgacctggct tatgtccccg tggccggtgc ccgccttaaa
     2461 ggcgcttttt cggtcgaagt gaagacgcgg ggcattacgg cccagcagaa atcaggccgt
     2521 tgctggctct ttgcggcttt gaatatcctg cgggaaaagg tggccgaaac gtgccacctc
     2581 gaccagttcg agctgtccca gaattacttg tctttttacg ataaattgga aaaagccaac
     2641 aatttcctgg aaatggtcat cgacaacgcc caggaaccca taaaaggcca gctcatgcag
     2701 tacgtcctgc ggggcatgac tgacggcggc tattggagcg aagccgtgga cctcatcgaa
     2761 aaatacggcg tcgtgcccaa acaggtccag ccggaaacgt atcagtccaa ccacatagac
     2821 cgctttgtag cgacattgaa ccgcctcctg cgcaaagatg ccatggagtt gcgggaactc
     2881 gtccaggacg gtgcggaccc gtatccgcgc aaggcggcca tgatggccga agtctacaag
     2941 gctgcctgca tcgcctttgg ccagcctgtc cagacctttg acttcgccta ccgcgataaa
     3001 gacggcaact accatgaaga gcgggacctg acgccgcagg ctttctatca gaaatacgtc
     3061 ggcctgtccc tgcgcgatta tgtcgccgtc atcaatgaac cgacggcaga taagccgctg
     3121 gatacgccca tccagttcca tgccgtcgaa aatatggccg gctgcgacat gaaggccctg
     3181 aacctgtcgc aggaagccct ggaagaactc tgcgtcaaac agttgaaaaa cggtcaggcc
     3241 atctggttcg cctgcgatgc cggggccttc ggcgcccgca aggaaggtat ctgggacccg
     3301 gccagcgtcc agtacgaagc ccttttgggc ggcttttcga cggccatgcc caagggcaaa
     3361 cggctggagt ataacagtag ttcggcgact cacgccatgt tgctcacagg cgtccatttc
     3421 gacaaggaag gcaagccgga ccgttggaaa atcgaaaatt cctggggcaa ggatgtcggc
     3481 gacaaaggct atttcgtctg ctctgaagct tattttcagg aattcgtcta cgaagccgtc
     3541 atcgaccgcc gtcatttcag cccggcccaa ttagccatgc tggaaaaaga ccctgtcgtc
     3601 atcaacgcct gggacgaaga ttattagaag ggaaggcttt tatgtatacg tttgatggca
     3661 aggcctatga ctgccccttc gattgtttca tcgacatcat caaaggcaaa tggcggacgt
     3721 ctatcttact ggctctggca gatgggccgg tgttcttcgc caagctgcag cggtgcctgc
     3781 cgggcatcag tgccaaagtg ctgacggaga acctccacgt cttcgagcgc aacggcctgg
     3841 tccggcggaa cgtccatgcc acggtaccgc cgactgtcga atacagcctg accgacagag
     3901 gggaaaagct gatccatgtc atgcagggca tcaacggctg ggtcgatggc ttttttggaa
     3961 aataaaaaga gactgtcttc gtgcgacagc ctctctttgg ggaagcgtta gaacatcgta
     4021 ttgtcgttgg ctgctttttc cgggatatcc tggatgacat cccagtgttc tacgattttg
     4081 ccgtctttga tgcggaaaat gtcgacagca gcctgaccac ggtctgcttt attgagtttg
     4141 atgtgattgt ggatgaagac
//
LOCUS       DAWABZ010000150         4148 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_5197_length_4148_cov_3.286098, whole genome shotgun sequence.
ACCESSION   DAWABZ010000150 DAWABZ010000000
VERSION     DAWABZ010000150.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5197_length_4148_cov_3.286098"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          964 a         1187 c         1059 g          938 t
ORIGIN      
        1 cgttgacatt gggcgatacg gaggccatgc cttcgccgac catggccggc gtattgggca
       61 tgacccggac gattttagtt tccggaccgg tgtaaaaggc cagcttttcc aaagagatgc
      121 cggcggcgat ggaaatgagg acgctatcgg ccggaaggga ttcccggacg gcttccagtg
      181 ccgattccat gatgttcggc ttgacggcca tgatcaggcc ctgggactgg cgtgccagtt
      241 cttccggcgt gtcgacgacg acagctcccg tttctttggc cagggcttcg gccttttcct
      301 tgtggatatt ataaagatag atatggtctg cagcaatggc atgggcagct accaggcctt
      361 tgacgatggc ccggcccata ttgcccgtcc cgcagacacc gattgtctgg aacataggaa
      421 gcctcccttt ccgatacgga cttactgttt agcgccgacg gcatccaagg tcgctttaat
      481 cttggcagcc aggccgtgga tcttcttctt ggaaacagag cagacagcca ggcggatacc
      541 tttgcccaag ggaacgagga agatattttc tttttccagt tcatcgcaga ctttctggga
      601 cccttccata gggattgtga tgaagaagcc cgaaatgtaa cgcaaatatt tcaagccaca
      661 ttcatcggct tctttcatga acaagtcagc ccgttcctgg atgaggtgga aatatttagc
      721 ccgttccgca tcgagttcag cgactttggc cgggtccttg cagatattgg ccatgacctt
      781 catggcagcg ctgttggagt tcgaccaggt agcgcggctg gtatactgat tgatggcgac
      841 gaattcatcg gcaatatcct tgttcggcgt gacgcagatc atggcgccca tacgctggcc
      901 atacatggtg aagcctttac tgcaggtgta ggcgacgacg atgaggacgt tgtccggcat
      961 gttgccgaat ttcttgaaga atttgcggca ttcatgtttt tcgccgctgt agtcgagata
     1021 agcgacgtcg gagacgaaga tgatgttctt atccttgcct ttgacgacat ctttcaagaa
     1081 ggccaggact tcgtcccatt ccgaatcgga gaggccgtaa cccgtcgggt tgttggccgg
     1141 cgaattcatg atgataaccg tgttgtactg tttggcaacc atgtcgctga cgttggcttt
     1201 gaagctttcc atattgaaat ggccatcggg cgtgagcagt tcaaattcac ggagtttgcg
     1261 gccattatcg ttgcacatgg aaccgtaagc cccccaatgc cagtcactgg tcaggacttc
     1321 atcgccccat tcggaatagt tgtgaatgac gtgatggagg acaccggaac cgccggacgt
     1381 agcacaggcg cggatgtagc cttccggacg ggattcaccg aagcactggt cgatagcagc
     1441 ttccaaataa tcgggatagc cctggatcgg cgcataggcg acaatttctt tcggcgtcaa
     1501 ggctttatag gctttctgga cgacgtcgag catgatgaga ttcccatctt catcgagcat
     1561 ggaaccgact gtcccattaa taactttatc tgcccccaat ttttcggtca aagctacggc
     1621 acggttgtta gcaccaaaaa tcttatcttc agcactcttc ccttttgcct gcggtgcggc
     1681 aacacttttt accatatgaa tcaactcccc tttcttatgt acatatacat ttataccaca
     1741 ttcaaaatcg aaagacaata aacatattgc atatatctgt catgtattca aaatacaaaa
     1801 ggaataacgc ctcttggaca atcaaagtta atcccatttg ctatcttagc ataaaacggt
     1861 tgaaattata tcatgactct cctataataa agagtatata acatatttcg tgttactttt
     1921 atgcattttt attctatgaa aagaggtctt tcccatgaat cgtgtattac gttttgtatt
     1981 agccgctgtg ctggcctgct cggccctcat gctgacagcc tgccagggca gcccgtccgg
     2041 ctcgaatgac aagaagacgt acaaaatcgg cgtcctgcgc atcgacgaca gcctgcccct
     2101 ctatacggca gaaacagaaa aactctttgc caaacacggt gtcgacgtcc aggtcattga
     2161 attcagctcc gctgcggacc agcaaaaagc catggaagcc ggtgaactgg acggcatgat
     2221 gaccgacatg atcgtcacgg ccttgctgaa aaaagccggt acagacgtcc gcgtcgtcgc
     2281 catggctctc ggtgccgtcc cccaggaagg ccggttcctc gtcgtatcgt cgccgaacag
     2341 cagcattacg acgccgcagc agctgaccgg tgcctccgtc gccatttcag aaaataccat
     2401 gatggactat ttgatgaacc agtatgaaca ggaactgggg attccggccg ccgaagtcca
     2461 caccgttcag atgccaaacc tggccctgcg cacggaagcc gtcctggccg gtaaagatat
     2521 ccaggccgcc atcctgccgg acccgctggc agcctatgcc gtccagcagg gagcccacgt
     2581 cgtcatcgac gatacgaaac tccaaaagaa ctattcccag tccgtcgtcg tcctgactca
     2641 ggacatgatt gaaaagcatc atgacgacgc agcccatttc ctcgacgctt acaatgaagc
     2701 gatcgaaaaa ctggacagcc agcccgatgc ctatcgcgac atggctatga aaaaggccaa
     2761 catcccggcc gccgtcgccg gttcgtaccg tacgccgtct tatacggccc acgccctgcc
     2821 gacagaagaa gaagtcagcc gtatcatgaa ctggatggta gaaaaaggcc tgttgcccaa
     2881 ggcctacagc tatgacgaaa tggtcgccca tgtccagtaa tcagcctatg gccggaccgc
     2941 tgctgcgctt agacgacgtg ggcctcgcct atcacagccg caggggcatc caggccgtgc
     3001 tccagcacat ctcattcagc ctcgcccagg gagagcacct gaccgtctcc gggccgtcgg
     3061 gctgcgggaa atcgccactg ctgcgcctca tcgccggcct gcaggcaccg acagaagggc
     3121 agatttattt tgcaggccaa aaaatgaccg ctcccaggcc ggacctggcc atcgtcttcc
     3181 aggactatgg ccttttcccg tggaagacgg tcgaagaaaa tattctcctt ccaggcaggc
     3241 tccacggttc tcccctggat aaggaccagc tggcctccct cttggacacg ctgggcctga
     3301 cagccgtccg ccatcactat ccgggggaac tctcaggcgg ccagaaacag cggaccgccc
     3361 tgggccgggc catggccatg cagccgaagt tgctgctcct ggatgaaccc ttctcagccc
     3421 tggacccgga catgcgccgt caatgccatg acctcttcca acaatacatc gtaaaaagtc
     3481 agaccgcgac gattctcgtc acccacagtc cggaagaagc agcggccctg ggtgacaaga
     3541 ccatcgtctt cgccaggaca ggaggtgtca tcgaagatgt ccggaaaaac aagtgatccc
     3601 atcgatatgg cccatctcct gctggccttt ttcatcatcg gcgccttatg gcaggcggcc
     3661 agcatggtac tgaacaagcc cttgctgccg gaaccgtacc gcgtctgcca gacctggtat
     3721 gccctgatga cgacaggcgt cctttggccg cacatcgcat ccagcctgta ccgcatggaa
     3781 gtccgcaact atgaagaaat gtacgccgcc atcctgaccc tggccgccat cggcatcatc
     3841 gcctacatgg ccctcgatat agccgaacga aaactctgcc cgtggaagaa gtagccgtca
     3901 gctgttaatg actatcacct gtatgcgata tcgtaggggc ttgcacgtgg caagcccgct
     3961 atgaccatgt gagcataacg tatgctgata tatgtgcgtg ttttcctgca tcacctgtca
     4021 tgtatgaggg atataccatt attccgaacg ggcgccccac gtgggcgccc ctactagcag
     4081 ctagcagtga gcagtcccac gagccacgaa ccactagcca ctattttccc acctctcctg
     4141 ccgaatcg
//
LOCUS       DAWABZ010000151         4102 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_5262_length_4102_cov_3.466271, whole genome shotgun sequence.
ACCESSION   DAWABZ010000151 DAWABZ010000000
VERSION     DAWABZ010000151.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5262_length_4102_cov_3.466271"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          766 a         1122 c         1226 g          988 t
ORIGIN      
        1 cgttccagca accatcttgc gccttccgtt ggtcggcacc cgatgggctg gaaaggcttc
       61 aggacggccc ctacgaacca cgaacaactt ccttcaggaa ctggcggaac ggggcggcca
      121 ggtccgggcg gcgcagggcg tattcgacgt tggctttgag gaagcccagt ttatcgccta
      181 cgtcgtagcg tttgccttca aagcagtagg catagacggc ttcgcggctg gccatggtct
      241 gcaaggcgtc ggtgagctgg atttccccgc ccttgcccgg ctgggtatgg gccaggattt
      301 caaagatgtc cggcgtgacg atatagcggc ccaggacggc cgtccggctc ggtgcttctt
      361 cgatggctgg cttttcgacg acgcggttga ctttcaggat gtccttccct tcgacaggcg
      421 tcccctggac gatgccgtaa gacggcactt tttctaaggc cacttcctgg cagcccagga
      481 tggtcgcatc gaggtcgttg tacaagtcga tcatctggcg cagggccgga ttggtttcgt
      541 tgtagacgac gtcgtcgccg aggaggatgg caaagggttc atcggcaatg aaggactggg
      601 cgcagagaat ggcgtcgccc aggccccgca tgtgtttctg acggatgtag tggatattga
      661 tttccgagat actgcggacc atgttcagaa gccccatctt atggctttct tccaggtgca
      721 gttccaggcc cggattggta tcgaaatggt cttcaatggc ccgtttagca tggccgctga
      781 tgatgaggat ttcttcgatg ccgctctgca gggcttcttc gacgatatac tggatggtcg
      841 gtttatcgac gataggcagc atttctttcg gtgtcgcctt cgtcgccggc aggaaacggg
      901 tgccgaaccc ggccgccggg atgacggctt tacggatttt tttcacaacg atgctcctta
      961 ttcaaaccag cgggacaaat cgaattcttc gccaatgatt tccttgacga agccctgagc
     1021 caagtacagg cccaggagcg acagcgtcga tacgcgcagc agcgagctgt cccagatatc
     1081 gcccaagtcg gccaggaccg gcgaaatatc ttccatgatg tccagggctt cttcgccgct
     1141 gaagttggcc gggcgtttct tggccaggcg caggatgtgg tcctgcatgt tgcggtcgtt
     1201 gatgccggtc atgcggaaga agcgtttggc catgctttca tcgaccatgg ccagtttgac
     1261 cagtggcgaa ttgaaggacg ggacgatgaa ttcatcgggg atgcgcttgc ctttgagggc
     1321 tttgcgcaat tcttcttctg tgaaaccgcg gtagcggaac aagagcggat acgaatcgga
     1381 caggatttcg gcaaaatgcg tttccttggc ttcaaaagcc gtgcagtgca agtaatcgag
     1441 ctgctggtag taaggccgtt ccgtcgggaa tccatccagg aagggataga cgtacttgtc
     1501 gatatagcgg cagaacgaat ccttgtcgac gttatgggag ttgcgcgaat acaggaacaa
     1561 taagagaatc gccaggatct ggtaatggcc ctgggtcaaa tagggcatga ccttcaaggc
     1621 atcgatggcg accagatggc gcgtcgaaat gggcgagcag tggacgatat cgtaaaaggc
     1681 ctgcagggca atggtgccga cgtaatcatc gggatgagcg gcataggctt tctggacgtt
     1741 ctgcaaggct tcctggacga tgagcggtgc cagggcctgc tgccgttccg tatcggtctg
     1801 gcagccgatc tggtcaaagg ccgccgacgt gatgtattcc gtccgctgct gcagttccgg
     1861 tgaaacgacg ccgtactggc gcaggaagtg agagacgcac tggcggacga caggcgaatc
     1921 gacagtgctt tccgattccc atacggacgc atagtgcggc gcattgccca gtgacgggtt
     1981 gcgcgtatcg gtcccggacg tattggaatc ccacgacgtc tgggacggtt tgacgaccgg
     2041 tgccggcttg acgacaggtg tcgctttttc ctgggacttg tgggcccgga tgggcggcat
     2101 aaccatcgtt tcttccggga cggctttctt cttggccgtc atggccgagt cgatgtggat
     2161 cggttccatg cgctgggtct gttccagctg ggaagcgacg gctttcatgc cttcgctgac
     2221 gacaggtcct tccaggacct gtgtcgcttc ggatacggcc cgtttcttgg cggcagccgg
     2281 tgcgtcggtg acggctttgg ctgtgccggc ggctttggcc gtttcggcca tgacgtcgcg
     2341 gagaatgtgc ttgcggtgcg tgtctttcgg cgcttctgtt tcagatgctg ccggttcagc
     2401 tgccggggca gcctgtttcc tggcggccgg ggcggcgccg ccatctttag cggccaggtc
     2461 agccgagacg atggcggcca tatcagccag gggcggcttg tcttcttcgg tctgggctac
     2521 ttcttcggcc cagcgtgccc gttcttcccg gcgggcattg gcttccttgg ccgcagcggc
     2581 ttcgcggcgg gcctgttcgc gccgttcccg gttgcgggcc agctgctgtt cctgggcttc
     2641 tttttcctgt tcggcctggc gctgacgggc tagtttggct tcttcctcgc gggccttata
     2701 ggcagcggct tcttctttgt ctaccttttc catggcttcc aggatttcat cgcgggacag
     2761 gacgcgcgtc gcatcgaggt catccgcatt ggggcccttg tggaccggag gttcctgggc
     2821 tttgggagct tctttataga cgattcggct attgcggcgg acggcttcgc ctttggtcgt
     2881 gacaaaggcc gtttcttccg gatgctcccg ccgttcgccc agtgtcattt tcgggacctg
     2941 cggcaagggg gctgtttctt cgtcgtccgc ttccttcggt aattgtctgg catgcttaac
     3001 tgttgccggg ctgcggtgct ggaccctgcg cagcggctgc ggctccccgt cgtagcgcgt
     3061 cttcgtcttc gtgttccctt tcttctccgt ttggggacta ctctcggagt gccctccgcc
     3121 ggaatacata atataacaaa ttacaaaaaa gacgattgct atgcccgcta tgatagcgta
     3181 cggcaaaaag tttgtcatgt catcctctcc tctattttat caaacttctt tccccattgt
     3241 agcataagat gacggcatga gctactccct attgccgatt aattcgcact ttcgtataat
     3301 aaaagagtaa actgattttt tgtccttcaa aggagtcttt ttcatggaaa aatataaagc
     3361 catcgtcttc gacatggacg gcaccgtcct ggacacgctg gacgaattga cgaaaagcct
     3421 gaactatgtt tttgcccgtc acggcctgcc cgccctgacg caggatgcca tccgtccctg
     3481 cctgggcttc ggctacgacg gcttcatcga caaagccgca ccggacgcgc cggaatccct
     3541 gcggcggacg atgacggaaa agttcatcgc ctattacagc gcccactgcc agacgacgac
     3601 ctttccctac gacggcattc cccaggtcct gggccggctc aaggcggccg gctaccacat
     3661 ggctatcgtc tccaacaaag gccagggtgc cgtatcggaa ctgcacgatg aatacttcaa
     3721 agactatgtc gatttctcct tgggtgaaac gccgatgtac cgcaaaaaac cggctccgga
     3781 catggtctgg gaagccctga agcgcctggg ctgtgccaaa gacgacgcca tctacgtcgg
     3841 cgactcggaa gtcgacaaag aaacggccga taacgccggc ctcgacgcag ccctggtcac
     3901 ctggggtttc cgcgaccggg acttcctgga atccctccag ccgacatacc tcatcgacac
     3961 gcgggaagaa ttgggagaac tcttcggagt gtgatttgag ttttaagttt gcagtttgta
     4021 gtttttagtg aatgctttta aatttaaagc cattttctac aaactacaaa ctccgtacta
     4081 caaactataa agagaaaggg gc
//
LOCUS       DAWABZ010000152         4099 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_5268_length_4099_cov_3.586053, whole genome shotgun sequence.
ACCESSION   DAWABZ010000152 DAWABZ010000000
VERSION     DAWABZ010000152.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5268_length_4099_cov_3.586053"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          911 a         1105 c         1178 g          905 t
ORIGIN      
        1 gtgtctatgg gcgcaatgaa tcgcgtattt ttgccaaacc gatcgtagag ggcaagtgtc
       61 tctatggcat gagtgaagaa gaaagggaaa gcctggacaa cgcattgaat tacagcggtg
      121 acatgcaggc tgttcaaaag gatctggaag gataacaaag aggggaaaag cgtgaagatc
      181 cacatcaaga tatatccatg gatgcgcaac ctgcaatcat ggtgccaacg catgaaggcc
      241 tgggaaacac cggcatggtg gccgtcattc ctgaaaacgg accggacgga gccattagtc
      301 gtgacaccgg cgcaatataa gaaactcgtc cgcggctgtg ctgtcgcagt ggtcgtcatt
      361 gtaatcgctg caggcatcgg catttacgag ttcgtttcgc ttcagcaggc caaggcccag
      421 gaagcactgc atgaacagca gctggaattg atgcgtgaaa agacgacatc gctccaggaa
      481 aagatggata aaatggattc cctggatcag gagctgcggc aaatggtcaa ggggtcgggg
      541 gctggtgaca gcccaaaagg tgacggtggc gtagcagggg gacaaaagaa atctccggac
      601 ctttctcatg ccagctacaa tgacctgtta ttggccacct cccgcttgga aacccgcagt
      661 aacgcccgca tcatcagctt catcaccttg aagacggtct tgagcgatac ggcaggcgaa
      721 caggttcggc aaatgcagca gattacgcat aaatatcagg gtggtgccct gggcggcgga
      781 aataatggcg atgccagcag cacgtcgacg acgccttcca tctggcccgt cagcgggaca
      841 gtcacgtcta acttcggtta tcgcggcaat cctattggcg gcggttccgg cttccatgaa
      901 ggcatcgaca tcggcgtcga ctacgggacg cctgtccggg cgacggcagc cggtaaagtc
      961 accatggccg gctgggtcga cggctatggc aacctcgtcg aaatcgacca tggcaatggc
     1021 ttcgttaccc gctatggcca taactccatg cttttagtca cagtaggaca ggaagtccgg
     1081 gctggctcga tcatctccct ggccggcagt accggccgca gcacagggcc gcacgtccat
     1141 tacgaagtcc gcgtcaatgg atccccgacc aacccgctgc tgttcctgcc gtagagatta
     1201 gcgcatgccg gtctttgtca tcatgcacgg tctctttgtc ttcaaagttt gtagtacaaa
     1261 gtttagagtt tgtagaagat ggttttcaat tttttaccat ttgctgtaaa ctaaaaacta
     1321 caaactcaaa aggggactgt cgcacgtgcg gcagtccctt tttttactac tatcccccag
     1381 gaaggaggcg cgtttatgaa gagaaacatg cagatactcg gcttttgtat gctttttgtc
     1441 atccttctct tgagtggctg ttctttgatg ccagccaata caggggtcct catcgggacg
     1501 aacctggcct tgagcggcaa gggtatgtcc tattcgacat cgacggaacg gggcatcgaa
     1561 ctggccaagg atatggtcaa tgaccggggc ggcctcttag gtaagcccgt ccagctcgta
     1621 tcggtcgata atcatggtaa accggaagat gccgtagccg ctatccagca gctcacggtg
     1681 cgccatgtgt cggccatcat cggcccggac ttggccgatt gtacgcgggc tgtcatcccc
     1741 aacgtggaag ccgtgaagat tcccctcatc agtccggcct gcacacagcc ggatatcacc
     1801 gtcgatgccc agtcccatga ggtgtaccgc tatattttcc gcgccgctta tatcgatgct
     1861 tcccaggggc gggccatggc cgactatgcg ctgaaacagc tccatgtcaa gcgggcggca
     1921 gtcttttatt atccggataa gatgtatgcc cagggcctgg ccgaatattt ccgcagtgcc
     1981 tttgctgcca gcggcggtga cgtgcccgtc tatattcccg tagaaaatgt actggacctg
     2041 actaaatacc tgcccttgtt gcagcgggaa caggtcgacg tcatctatct gcccggttat
     2101 gatgactgga cgataccggc tatcgagctg ctgcgcagtc acggcgtttc cctgcccctc
     2161 ttagggccgg acggctggaa tggccccaag atggaacggg atgtcgacaa ggattatttg
     2221 acccaggtct attatacgga ccattatgcc ggccacgacg cgacggatgc ggccaagcgc
     2281 ttttctcagg cttattatga aaagtatggg gaatggccgg atagttatgc cgccctgggg
     2341 tacgacgcct tcatgatgac ggccgatgcc attgaacgct gtcagtcagc cgaagcggcc
     2401 gatgtggccc gggaactggt gaagaccatc gattacgatg gcgcgacggg gaccatttcc
     2461 ctcgatgcca atcacgatgc cattaaagac gtcttcatca tgaccttcca agatggccgg
     2521 ccggaactgg cggcgacgat tcccgctgac catcagacac agtaaggcag atacttcgcc
     2581 atggcctggc gccgggcttt ccagtcgggc atgcggatgg tcatggcgtc atagaaatgg
     2641 cgggaatgat tgggctggag aaaatggcag agctcatgga gcatgacgtg gtcgatgatg
     2701 gcttcaggca tgatggccag gtaggtgttc atggtgacga tgcctttgag gggcatgcag
     2761 gacccccagc gcgaccgcat gacccgctgt ttcaggaccg ggtcgggcag ggcgaggccg
     2821 gcgaataagg gctgcatacg ggacaaactg gcaggaaaga gtttcttccc cagggcttgc
     2881 agcagcttat gatagagctt ctgccgcatg accggcgtgt catcggccag ttccatgaag
     2941 accgtctggc cgctgcggcg gatgctgttg tgcaggccca ggcggacgtc gagcctgtag
     3001 ggctggcctg ccagatagag cgtgtcgccg tcgctgaggg tcagtatggg gaatttctgt
     3061 ttccgttctt ccatttcgcg gatggcgcgg atgatgaagt ccgtcttctt ttccaggaaa
     3121 tcctcgatat aagcccaggg gacgcgggcc ggtgccgaca ggtacagcgt gccgtcgctg
     3181 cggacgtgga gattgatgtt tttcactttc ttgcggacca ggatgaccgg gaaggtccgg
     3241 ccctgggccg tgaactggtg ttgttcgctc gtcatgggaa tacctcgcag aaataagatt
     3301 tgtagctatt gtaaatagaa ttcgttcgtt tgtcaaaaag gaaggtagat tatgctcgat
     3361 tttacggcta tcgactttga aacggccaat aaatacgcca acagtgcctg ctccctggct
     3421 gcggtcacca tgaatgacga ccgctgtgtc aaagaaggct ataccctcat ccgaccgcct
     3481 ttcatggttt ttgattcagg caatatccag atccatggca tcacgccgga ccaggtcgcc
     3541 cataaaccaa agttcgatgc cctgtgggac cgcatccgcc ctcatctgga aggacgcatc
     3601 ctggtcgccc actatgccgt cttcgatacg cgggtcctgc gcagcctgct gaagacgtat
     3661 cacctggaaa agccccaggc gtcctatgcc tgcactgtcg aaatctcccg ccgcgtctgg
     3721 ccggacttgc ccaatcataa actggacacc gttgccggtc acctgggcta ttccttccat
     3781 caccatcagg ccttggacga cgcccgcgcc tgtgcctaca tcgtcctcaa agccgcagaa
     3841 atcgtcggcg cttcgtccat ggaagaactc ctcaaggcga cgcacctcaa gttgaagaca
     3901 ctgtgataga gtttgtatta tggactttgt aggggccgtc ccaaagggcg gcccgtctgt
     3961 catggtggtg tacacgtaat gcatgacggg tgatgcgtga aaacatcctt catctgcccg
     4021 aatagctatg tctacaattc acagcgggac gcctagccac agctgtccgt tttggcagcc
     4081 gtcctgcgcc tcctgctgg
//
LOCUS       DAWABZ010000153         4097 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_5271_length_4097_cov_3.615537, whole genome shotgun sequence.
ACCESSION   DAWABZ010000153 DAWABZ010000000
VERSION     DAWABZ010000153.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5271_length_4097_cov_3.615537"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          799 a         1299 c         1048 g          951 t
ORIGIN      
        1 tttttccatg atgtatccct ctctgattta taagcctatg caatctaaat atcgtaacat
       61 aatacttaaa acatattata cattataaag tataaaaacc tcaagcaaag atacaccaat
      121 accaaaggaa gacctcctca tccaccttta tcaagagctc cagcattcct ccgtttcggc
      181 aacggccctg cactttccat cggccagcac ctgacggggc aaaaatattc ttcccataat
      241 agctcacaaa ccaatagttt atcgcctacg agattctcac agcgagacgc cagccactgc
      301 tgtccgtttt ggcaactgtc ctgagctttc cgttggtcgg cccctgacgg gccaaaaagg
      361 ctctggacgt ctcctacgca caaggccatg ggccgtgtat ttttttgcgg tccgcaggac
      421 cgcggcgacc tgcgaacggc gaactgcggc ctgcgttccc tacttatgtt ccgccagaaa
      481 aacttcggca tcgtggcgga ttttttctaa gtccaggccc tggtcgcggg caccgaagat
      541 gcagccacag tagggctggc ggtagaggtg gtaggcttcg gtcattttcg tcgatgtgag
      601 gaagccgttg ttctttttga agtccgtcgg caaataagcg tagccgtact ggtcttcgat
      661 gtcccggccg acttcgttga tgacctggga attcttatgg ggactgacgg tcagggtcgt
      721 cgtaaagtaa tcatagccca gttcctgcat tttttcggcc gtcgctttca tgcgctggat
      781 gaagcagacg cggcagcggg cgccgccttc gggttcgtct tccaggccct ggacagcttt
      841 gaaatacgta tccggttcat acggagcggc caggaactgg acggcgtgcc ccgttttttc
      901 attataggcc gcgatgaact gtttctggac gagttcccgc cgccggtatt ccgcttccgg
      961 atggatattg gggttgaaat agtagaccgt cacgtcggcc acgccggtca gccggtccag
     1021 gacggccgta ctgcacgggc cgcagcagga atggagcagg atgcgcggct tgacgccgtc
     1081 ctgctgccag cgccgggcca tgcgctggaa aactttatcg aagttgatcc gctggttttg
     1141 attcatgcgg tccagaatat cgtgaaaatc aatcatgctt tgtactcact ttctatagat
     1201 tcaatatcat attatatcat ggaacagagg cctccgcatt ttctatcccc cctatgggct
     1261 tatatttttt gctgtttttc agtaaaatag ggataatggt tattttatta aggaaaagag
     1321 gtctactcat gcatatacct gaaaattatc tgagcccttc gacgtgcggc gtcctcggcg
     1381 ccgtcatggt tcccatctgg ctgctctcta tccgcaaggt caaggaaacg gtgccccgtg
     1441 aaaagctgcc cctcctcggc attgctgcgg ccttttcctt cctggccatg atgttcaaca
     1501 tccccctgcc cggcggtacg acgggacacg ccgtgggcgg tacgctcatc gccatcctct
     1561 tcgggccgta cgcggcctgt ctggccgtca gcgtggccct gctcatccag gccctgttct
     1621 tcggcgacgg cggcatcctg gctttcggtg ccaactgctt caacatggcc ttcgtcctgc
     1681 cctttacagg atatttcatc tatcacggcc tgaaaaaagt catcccgtca gaaacgatag
     1741 ccgccggtat cggcgcttat atcggcatca acgccgcggc cttctgtgcg gctgtggaat
     1801 tcggcatcca gcccctgctc tttacggacg gagccggcca ggccctgtac tgcccctatc
     1861 cgttatggat ttccattcca gccatgatga tcggccacct gaccttgttc ggcctggcgg
     1921 aagtcgtctt cacgacaggc atcctgacct atctgcgcaa gacgtcgcca gacctcttcc
     1981 atgcggtcca ggccccgtcg tcgagcaagt ccctcttcgg actcctggcc gccctcatcg
     2041 ccgccacgcc gctgggctta ctggccgccg gcacggcctg gggcgaatgg gatgccgaag
     2101 aactggccga tacggatttc ttcggctctg ccctgggcta tacgccatcg ggcatggaac
     2161 agggcttttc cttcgacgcc ctcatgccgg actacgccat cgccggcctg cccgatgccg
     2221 caggctacat catttcggcc atcgtcggca cagccctgct catcatcatc ttcaaagtca
     2281 tcgcgtcctt cctgccatcc cggccgacct tcgatacgcc ggcccagcac tagggaggat
     2341 tgcccatgaa gctccctgaa tgggcggccc gcgaggaaat atataatccc ggcagcgacc
     2401 gggattattt catttcccgc tccctgctcc gcctcgtatc catcctgacg gccctgcgca
     2461 gccagggcct gcggccaacc cgtcccatcg gcgcggcgcc ggccttattg gcaaccctgg
     2521 tcatggtcct cttcgtcgtc ctggcccgga cgacggcctt cctctgggcc gttctggcag
     2581 gcgaactggt cctcctctgc ttccatcgcg gccggcagct gcgccaagtc ctcggcgccg
     2641 ccctggcagc cgctctgttc tgcgccgtca tcgtcgcgcc gtccttcttt ctgggcaacg
     2701 gccgctacgc cctgctcctg ccctgcaaga ccttcctcac cgtgacggcg ctgatactct
     2761 tgcaggaaaa cctggcctgg cacgaactga ccggcgccct gcggaccttc cgcatcccgt
     2821 cgctggtcat tttcatcctg gacacgacgc tgcgctacat cgccatcctg ggccaggaag
     2881 catcggaact gctgacggcc ctcaagctgc gttctgtcgg ccacaatccc gataaacaag
     2941 cggccatgag cggcgtcgcc ggcatcatgc tgcaaaagtc ccagcgcctg tcccaggaca
     3001 tgtatgaagc catgcgctgc cgctgtttca ccggcgaata cgctgcctat gacgacggcc
     3061 agaaacgccg tttcacgccg gctgcggccc tgctcatcct ggttctgctg gcctatgcct
     3121 atctcttcat ccgcctggaa ggagtgttct catgattcaa ctcgacgacg tctgtttcgc
     3181 ctatgacgac aggcccatcc tcagccacct tacagaaacc atcgaaccgg gccaggctgt
     3241 cctcctgagc ggtcccaacg ggtccgggaa gtcgacgctc ctgcgcctgc tgaacggcct
     3301 gattttcccc cagcagggga cgtattcctt tgacggcaca atcatcacgg ccggcaagat
     3361 gcgggaccac gcctattcca aatggttcca ccagcgcgtg ggctacgtct ggcagaatcc
     3421 cgattcccag ctcttttgca gcagcgtccg cgaagaactg gccttcggcc cggtccagat
     3481 gggcctgaaa ccggcagaca tccgccagcg cgtcgacgat gccctggaac tcctgggcct
     3541 gacccggctg gcatcccgtc cgccttacac cctgtcaggc ggcgaaaaga aacggacggc
     3601 catcgcctcc atcctgacca tgaaccctca ggtctggacc atggacgaac cggaaagcta
     3661 cctcgacgcc gacggcctgg cctggctgga agacttcctg cccagcctga aagcagccgg
     3721 caagaccctc atcatcgcca gccatcacgc cgaccgcctg ggcgacctca tagataagga
     3781 aatagttgta ggggacgcct aaaaggcgtc ccgccggtcg cctgtcgcag gtcgcaggtc
     3841 gcagttcgcg gtccgttatg atatttcacc acatctcatg cttacatatg tcaaactttc
     3901 aaacgcaacc tcttgggcaa atgcaacctc tcagtcagct gtgctgacag ctctcctatt
     3961 aggagagcct ttgtgttacg ctttatttaa gctttttaac attttatcaa aatattttat
     4021 tgatttttcc gttataaagt tatataatcc cgagtttgac acttttgaaa ataaaaaatg
     4081 ctcacaaaga gcgtggt
//
LOCUS       DAWABZ010000154         4037 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_5367_length_4037_cov_2.866650, whole genome shotgun sequence.
ACCESSION   DAWABZ010000154 DAWABZ010000000
VERSION     DAWABZ010000154.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5367_length_4037_cov_2.866650"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          892 a         1104 c         1175 g          866 t
ORIGIN      
        1 cttcttcata cgtcgggtcg agcttctcca ggaccggctg gatcgagcgg acgacgaacg
       61 ggatgccgac gaagaccatg gcgatggtca ggccgatctt ggtataggcg atctggatgc
      121 catagctggc aaagaaactt ccgaagaagc ccgtatcgga atagagcttg gaaagggtga
      181 tgccggcgac agccgtcggc aaggcaaagg gcagttcgat gacgccgttc aagagacgtt
      241 tcccgaagaa atcgtagcgg accaggaccc aggccaggat gaagccaaag acgcagttga
      301 ccaaagccgc taggacagaa caggaaatgc tggtgatgaa ggcgttgcgg atgccgctgt
      361 tggtgatgag cttggcatag tcattggccg tcagctgcag ggagtgcgcc aggaccgacg
      421 ccagcgggat gaggatgagc agggacaaca tggtaagggt catgcccatg gacaggccga
      481 agccgggaat aatctgattg cgcccgccgg aacgggcaaa aaagtgtttc atccggctcc
      541 cctcctatgc atcgccgttg acgtcggcca tgatcttatc gaacaaggcg tcgtcgttga
      601 aaaatttaac atacgcagcc tcccagccgc cgaaatcgct gatcttggtc agcttgacct
      661 tgagatcgaa gcggtcggca taacgccgga ggatatccgg attagacggc cggaaaccgt
      721 tcttggcggc cagttcctgg gcctcatcgg aatagaggta ggccaggtat tcatgagccg
      781 cctcttcatg acccttgcgc cgggccaggc ggtcgacgac agccacgctg ggctgggcca
      841 ggatactgat gctgggcacg acgaggtcga acttatcggg atattcgcgc atcgaataaa
      901 gcgcttcgtt ttcccaggcg ataaggacgt caccttgtcc gttttcgaca aaggtcgtcg
      961 tcgagccgcg ggcgccggaa tccatgaccg tcacattgcg gtacaagtcg gtcatgaagg
     1021 cccgggtctt gatttcattt cccccgaaat ggtcaaggcc ccagctccag gcggccagga
     1081 agttccagca ggcgccgccg ctgctcttcg ggtccggcgt aatgacgctc acgccgggct
     1141 ttaccaggtc gtcccagtcg cgtatccctt tcgggttccc cttgcggacc aggaagacga
     1201 tggtcgacgt gtacggcgcc gaatcgttgg cgaaatcccc ctgccagcct tcatggatga
     1261 ggccggccct ttcgatgagc gacacatcgt gggccagggc cagggtcacg acatcggcct
     1321 tgctgccttc gatgaccgac cgggcctgac tgcccgatcc gccatgggac tggatgatgc
     1381 ggatatcctt gcctgtcgtc tcacgatagt gctgctggaa taattcattg tattctttat
     1441 ataactcacg ggttggatca tacgagacat tggtcagcgt catcgtgccg ccgcccccgg
     1501 cgtcatcgct gcgtccgcag ccagccaggg ccccggcgac ggcgagtgcc gccgaaaaag
     1561 ccagtatagc tatgatgcgc ttcttgttca ttttctttcc ccctctatac cggcttagat
     1621 tccttacccc tcaaataaag ccgtcgataa atagcgttcg ccgccatccg gcaggaggac
     1681 gacgatagtc ttgccttcgt tttccggccg gctggccagt tccagggccg cctgcagcga
     1741 cgcaccggac gaaatgccga ccaaaaggcc gtcggtcttg gcgacttccc tggcccggcc
     1801 gtaggcgtcc tcattggata cgtcgatcac atcatctacg agagatatat ccaaaacttt
     1861 cgggatgaac ccggcgccaa tcccctgaat ggcgtgcggg cccggccggc cgccgtgcag
     1921 gacggcggaa tctttcggct cgacggcgac gatctggacg tccggcttat ggcctttcag
     1981 gccctgggcc gtaccggtga tggtgccgcc tgtgccgacg gacgaaatga agatatctac
     2041 cttgccgtcc gtgtcgcgcc agatttcttc cgccgtcgtc tggcggtgga tggccggatt
     2101 ggccggattg tcgaactgct ggggaatgaa ggcattgccg taagaatccc tcaattcttc
     2161 ggctttgccg atgctgccgc gcatcccctc gatgcccggt gtcagcacga tttccgcccc
     2221 cagggccgac acgatcttac gccgttccag gctcatcgta tcgggcatgg tcaagatcag
     2281 gtgcaggccc ttcacggcgg cgaccagggc caggccgatg ccggtattgc cgctcgtcgg
     2341 ttcgatgatg accgtatctt tgttgatctt cccctgccgt atcccttcgt cgatcatggc
     2401 tgccgctacg cggtccttga cgctgcccaa ggggttcatg tactccagct tggccaggac
     2461 gcgggccttg aggccgtgcc gagcctgcca atgagtcagt tccaagagcg gcgtgttgcc
     2521 aatcagctct gtcaaatacg ttgccacatg ctccatgtac tcagctcctt tttggcaaaa
     2581 agaaaagcgc cgcctgctga tgcagatggc gctataaata gacgtatgcc catgacttca
     2641 tctgccaggt tatttcctgc tggaattggc accttaccta cgtaggttgc cgcagcgtca
     2701 tcgggccagt ccctcagctg ctctggatga ataggtttcg tattgtattg tccttatgaa
     2761 tggttgtaca atacgatcat attatacaac acatgatggt caaatttttt aagtacccca
     2821 tgataacata cctctttgat gactaaaatg aatagcttcc tatcggaaac gttaccaaga
     2881 gtgtacactt tacagagtat attgtcaagg gattttaagc aaaaactttc atattttaca
     2941 atgtaaacgt tatacctatg gctttagata ggaatttaac tttataaatt atatttttta
     3001 taagtaaaaa gggcttgttg tatatacagc aggccctttt tagggattag ccgttagtgt
     3061 ctaacagcta acccctaacc cctagctgct aactgctaac tactgactgc aaacttcgta
     3121 cagctgacgg ctgaccgcta acaccctcaa tgcgtcgccg tgggctgggc gtcgtgatgg
     3181 gccgttttgt tgtcggcgtg gacgagttct ttgccgcagt agtagacgat ggagatggcc
     3241 aggaccatga gggcgatggc caggacgaac tgcagtcctt cgatgaggaa cgaccagtta
     3301 cccgaggcaa tcttgatgat cagggcataa gccgattcga cgagggctgt catggtaacg
     3361 cagaacatga cgaccatggg gatgtagagc atgaagctct tgcggcctgt cgtcttgagg
     3421 aagaccgtca ggcagatgag gaccatggcc gacagcagct ggttggccga gccgaagaga
     3481 ggccagatgt tcatgtagcc gcctaagcag agcaggaagc cgaagaacaa ggtgatgacc
     3541 gtagcaaaat acttattgac gcagattttc tggatcgtgt tcatttcctg tccttcatcg
     3601 acgctgaaca gttcctggaa ggacatacgg ccgatacggc cgacggcatc gagggtcgtc
     3661 agggccaggg ccgatacgca catggtcatg atgcaggcag ctgccgtctg ggacaggccg
     3721 aacatggtga agaaaccagc gacggcgccg gagaagatct ggaacggcgt gcccgaagcg
     3781 acgtggccgt tggaagcggc agcgcaggcg atgaccaggg aaacgacgcc taagaccgat
     3841 tcgacgcaca tggaaccgta gccgacaaag accgaatcct gttccttatc cagggtcttc
     3901 gacgacgtac cggacgaaac gaggctgtgg aagccggaaa cggcaccgca ggcgatggtg
     3961 atgaacagga ttgggaacaa atccttgccg ccgaccgaga aggacgtgaa ggccggcata
     4021 ttcagcgtcg gctgggc
//
LOCUS       DAWABZ010000155         4029 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_5382_length_4029_cov_2.792904, whole genome shotgun sequence.
ACCESSION   DAWABZ010000155 DAWABZ010000000
VERSION     DAWABZ010000155.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5382_length_4029_cov_2.792904"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          837 a         1061 c         1121 g         1010 t
ORIGIN      
        1 cctttgaact ggctgtcccc gtccagacga aagaagatat ccaggccctg gaagatgcag
       61 ctaaagcctt ccacgaagcc catcacgacg caacggtcat cccacatcct atcggcaaaa
      121 tcgccaaaga agccatgctc cagggccaga ccttcggcgc gcccctgctc tatacgacag
      181 acggtgaaga aacggaaacc ctgccgacca gcacggccat ccataaactg ctccatccgg
      241 aagaataata taaaaaagag cttgtcgcac gacgacaagc tcttttaagt tgttagtggc
      301 tagcagttag ttgttagtag atggttttaa atttaaagtt tttctctgaa aactacaaaa
      361 tctgtactac aaactataaa gagaaaggga catcgcatga tggcataggc cttcatgcga
      421 tgtccctttt tagctgcaaa ctatctttta ctgtaaattc ttggtcaaca gctggttgac
      481 gacgccgggg ttggcccggc cttttgtttc tttcatgatc tggccgacga ggaagccgat
      541 ggctttcttc ttgccggctt tatagtcggc gacggactgc gggttggcag cgatgacttt
      601 tttgaccatg tcttccaggg cacctgtatc ggtgatctgg accagaccct gttctttgac
      661 gacgacgtcg gctgctttgc cggacttcca catttccggc aggactttct tggcgatctt
      721 gccggagatg gtccctttag cgatgagttt caagaggcca gccaggtcag ccggtttgac
      781 tggcgaagcg gaaatgtcga tgttttcttc gttcagtttc ttggcgaatt cgccgagcat
      841 ccagttggct gcttctttgg catcgacgcc ttctttgacg acggcttcaa agtaatcggc
      901 tgtctgtttt tccagggtca gcaagttggc gtcgtattcg gacaagccca attcatcctg
      961 gaagcgctgg cagcgggcat cgggcatttc cggcagttcc tggcggacct gttcgatgta
     1021 ttccggcgac acttcgatag gaatgaggtc cggttccggg aagtaacggt aatcgctgtc
     1081 cccttctttg acgcgctgga ggatggtaat gcccttggct tcatcccagc cgcgcgtagc
     1141 ctgctggatc gtaccgcctt cgtcgaggat ctgagcctgg cggacggctt cgtattcaat
     1201 ccccttctgg acggcgctga aggagttgat gttcttgatt tccgtcttgg tccccaattt
     1261 atccgaaccg acgggacgga cggagatatt ggcgtcacag cgcatactgc cttcttcgag
     1321 gcggcagtcg gaaacgccga ggtactgcat gatggagcgc agtttttcca gatacgctct
     1381 ggcttcggca ccgctgcgca gttccggttc gccgacgatt tccaggagcg gtacgcccgt
     1441 acggttatag tcgacgctgg tcgaatcaga gttggcaatg ctgccgccgc tgtggacgag
     1501 cttgcctgcg tcttcttcca tatggatacg ggtcaaatgg atgcggcgct gtttgccgtc
     1561 gacgtcgata tcgatgtagc cacctttgca aatcggcagg tcgaactggg acgtctggaa
     1621 gttcttcggc aaatcgggat agtagtagtt cttgcggtcg aatttgctga acggcaggat
     1681 gtcgcaatgc agggccaggc cggctttgac ggcataatgc aatacttctt tattgagtac
     1741 gggcaggacg ccgggcaggc ctaagcagac cgggcagacg tgggtattcg gttcggcgcc
     1801 gaattccgtg ctgcagccgc agaaaatctt ggttttcgtt tttaattcga catggacttc
     1861 caagccgata acggattcgt atttcattaa agttcaacct cccccatggg cgacggcttg
     1921 cgaaggtccg tccgattctg ttcaaacgta taggccacgc gaagcagcgt tgcttcgtcc
     1981 agggccttgc cgatgagctg catgccgatg ggcaggccgt tcttatagcc gcagggaatg
     2041 ctgaggcccg gcaggccggc caggttcaac gtgaccgtgc agacgtcgcc gaggtacata
     2101 gccagcggat tgtcgctgtt ctggccgatt ttgaaggccg tatccggtgc cgtcggcgtg
     2161 agcaggacgt cgcatttctt cagggcttcg tcgtagtcct gtttgatcag agtacggacc
     2221 ttcatggccc gcagataata agcgtcgtaa tagcccgagc tgaggacgta ggtcccgagg
     2281 aggatacggc gtttgacttc gtcgccaaag cctttcgtac gggtcttgat gtacatgtcg
     2341 acgatatcct tgccgtcgac gcgcatgcca aagccgacgc cgtcataacg ggccaggttg
     2401 gagctggctt cggccggcgc gatgatatag taagcagcga tgccgtattt catgtggggc
     2461 atgctgaccg gtacgatttc agcaccgagt ttcttatacg tttcgatagc gtcttccagg
     2521 gccttgcggc agtcagcatc gaggccttca ccgaagaatt cttccgggac gccgattttc
     2581 atacccttga cgtcattgac cagggcctgt ttgtaatctt tttcagcctg gggaatggac
     2641 gtcgagtcct tggcatcgtg accggcgatg gcattgagga cgatggcgca gtcagctacg
     2701 tcgttggtga aagtcccgat ttggtccaga gacgacgcaa aggcgatgag gccataacgg
     2761 gaaatgaggc cgtatgtcgg tttgaggccg acgacaccgc agtacgaagc cggctgacgg
     2821 acggatccgc ctgtatccga accgagggcc cagaccgttt cgcccgaagc gacagcggca
     2881 gccgaaccgc cggacgaacc accggggacg caatcgaggt tccacgggtt atgagtcagc
     2941 tggaaagccg agttttctgt cgaactgccc atggcgaatt cgtccatatt ggtcttgccc
     3001 aggctgacat aatcagcagc catcaatttt tcgatgaccg tcgcattgta cggcgatttg
     3061 aaattttcca gcatcttcga tgcacacgta gccttctgac ctttgatgca gatgttgtcc
     3121 ttgatggcac cgggaatccc ggcgaggtca ctgatggctt caccggctgc gattttggca
     3181 tcgacagcgg cagccgcttc cagggcatag tcgcggttat ccgataaaaa cgcctgtact
     3241 ttcggttcta ctgtatcttt atgtgcaaga aaagccttgg tcaattcgac agccgatacg
     3301 tcgccgttga ccagtttttc atgcagttca tggatggtta acatacatac gcctccctgt
     3361 taatcctgga tgactttcgg aactttgaaa tagccctcag cctgttccgg cgcattctga
     3421 agcgcttttt cattggccag tgacggctgg ggaacgtcgt cgcgcatgac gttggacatg
     3481 gatacggcat gggccgtcgg cttgacatcg gtcacatcga cttggctcaa gagatccatg
     3541 tacgataaaa tgtcgttcag ctgtttcccg accgtttcca tctggtcttc cttgacttcc
     3601 aggcgggaca acagtgcgat cttctttatt tcttcggcac tgattttcat actgacactc
     3661 cttgcagata tttttagcgt gacaaatcgg aatcatttgt caaaattcaa cacatatata
     3721 atttaatcac cttttgccat attttgcaag cctcatcgcc tgtatgcgat accgcagggg
     3781 ccgtcccaga gcctttctgc cccatccgat gcaggacgat tgcccgaacg gatagcagcg
     3841 gcgggcggcc cgctacgtgc tgagggcttc cccaagcatc gttgttactt tgagtttgtc
     3901 gcggcggaat gccgcatctg agtccgagga aaaagccgtg gctcgtggct cgtggttcgt
     3961 ggctcgtaaa aatccaccat tataccagaa tgggctatga tggaatctca cagcgggctg
     4021 ccctgcggg
//
LOCUS       DAWABZ010000156         3988 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_5439_length_3988_cov_3.132723, whole genome shotgun sequence.
ACCESSION   DAWABZ010000156 DAWABZ010000000
VERSION     DAWABZ010000156.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5439_length_3988_cov_3.132723"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          912 a         1080 c         1148 g          848 t
ORIGIN      
        1 aaactataaa gtaaacgggg ccgcgcatga tggtagaagc cttcgtgcga cagcctccca
       61 cggatcacca ttcactattt cgccggtgct tcgatagggt cgtaatcagc cggtttgtcc
      121 gtaatggatg cttcgtcgat ggccgacggt accgggtcga agccctggcg gaacagggcc
      181 ttgatggcag cgatgtacgg cgggatgatg gtgtccatga ggagatagcg cttgcggaaa
      241 aggttgcgct tctgcgactt gatttcgccg tatttgcgcc gtctttcttc gataggcagg
      301 tggtaatacc acggttcctt gccatccggt gcggctcctt cgccgatcca gaattcattg
      361 aaattggtct tcttcgagcg gttgccgcgc agcatatggc tgttggtata gaagccttcg
      421 tccgtgatga catatatgtc atggatgccc aaggtctgga cgaagatgcg catgagatag
      481 aggatgaaat tcttcggccg atagccaaag agcttcttgg tcaggacctt ggtcgtttcc
      541 aggccgtgct ttgagccctg gatggtgccg atgtacacgg acggcgtccc gtcaacattg
      601 taatcgaaac gaaaattgaa atgatagatc atctggcctt catgatagag gtagagcgac
      661 aagaagcctt ccttgcgctg gcccgtatcg aagatgagcc gcgcttccag gggcagcgat
      721 tcgtccggcg aagtccacag ggtatagccg ttcccccagt agagttcgtg gatgacctcg
      781 tcagaaaaga aagtccgcaa gatagtgaaa tgatgtttca aggcatcgag acgctggcgc
      841 atggtcgaat tcttgaacaa gaagacgcgg gtcatgactt cctggaaatc cgggtcatgc
      901 tgctccaaga agcccttcat cggttcataa tggtcaaaga aatcgtagag ctcttcaaat
      961 aaagcccggt tggacacggc acgggcaaaa aaaacgacag cccgcttgtt ttcctggaaa
     1021 ttccggggcc cgtatataag gcgcgataag tgccagaaat aggaaatgct gtgcatggaa
     1081 tacctcctat caagtacaaa gctaatagta ctattatacg ggcaagacaa gcaggctgtc
     1141 aatgtgggat gcgtggccag tggctagctt ctatctaatc cagcttccgc acgatattcg
     1201 ccgggacatg ggacaaatcg cggacgccgg tgatcatcat ggcatccatg agctggcgtt
     1261 tgagcgtatc caggaggagt ttgacccctt cggccccacc tccgacagca gcgacggcag
     1321 ccggtctgcc gatgaggacg gcgtcggcgc cgagggccag catcttgagg atgtcttcgc
     1381 cgtgacggat accgccatcg acgaaaatgg tgatccgccc tttgacggct gcggcgatat
     1441 caggcaggac atcggccgta ccggccatgc cgtcgaggac gcggccgccg tggttgctga
     1501 cgacgatgcc ttgggcaccg gcttcggcac aggagacggc ttcttcggcc gacatgacgc
     1561 ctttgacgat gaagggcagt ttgatagcct ggacgacttc ggcaatgtca cgggcactct
     1621 tgggccctac gggctgaccg aaaatccgca tgttaatcag cgtcgccgca tcgatgtcgc
     1681 tggcaacggc aaaagcgccg gcttcttcgg cctgatgggc tttctcgatg atctggtcgt
     1741 tgctgcgggg cttgatagtc gggatgacga tgcccggccg tttcttacaa gatgcaatgc
     1801 cggatgaata catatacgga gcaccgctgt caccggtaaa gcacagcgta cccacatcca
     1861 gggctgcctg agtgacggct tcgtcatact cttgttctac ttccgccggg tcgccgtcta
     1921 cctgggcatt gaggacgatg ccgccgaccg gcgccaggag taccggcata ctgagccgtt
     1981 ttcccataag ggtcacagcc gtatccgggt cgtcgacgcc agacatgctg cgcatgacca
     2041 ggccgtattc ctggagcgct tcgacgttac gcatgaaaga ccgtcccgtg cgcaggccgc
     2101 cgaaaccggg aatcatgccg acgcaggctt tgccgttgca caggggacag acatggcagc
     2161 ctttcatttt ttcgcgtgct ttgttcctta cttcttgtaa atccatgata atcacttcct
     2221 aaataaatat tttattgtca gcccggccct gataggtcgt ctctaacatc ttgacatggg
     2281 ataaaaaggc agggtcgtcg aaatacttgg cgccatgcgg gcagttgcgc acgcagcgct
     2341 ggcacttgat gcagatgcca ttgacgacgg acggctggtc cgggtcgatg gagcccatgg
     2401 gacactggcg ggcgcagagg ccgcaggata cgcatttttc caggtctgtc ttgggcttgg
     2461 ctttaaggaa cttggccggc tggccgtcga cgcctttggg gacgtaatac ggagccctgg
     2521 catcaccatc gacgacgaga cgcggtacca tgccatcggt ctgacggatt ttcgatacgg
     2581 ccatgtccgt caggcggacc atgagggcca ggtcgtcctt atcaggccgg tcaggggcca
     2641 ggacatcaga aaaagcatgg gtcccgacga aagccccggc tgcgacggta cggaagccgt
     2701 tgccgtcgag tgtcgcacag agctcggcca gggaattatc gaaagaccgg ttgccatagg
     2761 tcacgacagc cagggccagg gcgcccttgc cgatgagttt ttcctgatat gtcggcaaaa
     2821 tcttattggg cagtttcccg gcatacgttg gaccaccgac gatgaccagg tcaccagctg
     2881 aaaaagtatg caccatttcc cggtccttcg gcaggttcag ggaaatagcc cggcacggta
     2941 cctggaagag ccgcgccgct tcggaagcac cttgacagac gatgcgcctg gtattcccag
     3001 tggggctgaa ataaacggca tacacgtgtt ggatatgcat aaagaccacc tcgattataa
     3061 aaaatccggc cgcatctttt gcttatatta tataccaaac aggcgcgaaa gtcatagttc
     3121 gcgtctgttt tctttttccc ttttgtgtta gaatacagta tgattttact ataaaaacga
     3181 ggtgtcctat gcaaaagaaa atatatgccg tacggcgcgg ccgcacgacc ggtctgttcc
     3241 tgtcctgggc agactgcaaa aaacaagtgg acggcttccc gggagcccgc ttcaagagct
     3301 tcaccgatgc gacggaagcc tggaaatggc tcaatggcga agaaacgacg gcgccgaaaa
     3361 ggacaggagc tcctaaaaag tcccgccaga cggcaaagac cgcggcgccg tcccagccct
     3421 tgcggcctga cgaagaacag gatttcgtca tctataccga tggctcatgc ctgcgcaacc
     3481 ccgatggccc cggtggctgg gccgtcgtcg cctgcaacgt cgctactggc gaagtgacgg
     3541 aactatgcga cggcaatccg tcgacgacga acaaccgcat ggagctcctg gcagccatca
     3601 tggccctgcg ctttgcaccg gaaggcacga aagtcgcctt gtacacagac agccagtacc
     3661 tcaagaacgg catcaccaaa tgggtcgccg gctggaagcg ccggggctgg aagaaagccg
     3721 acggccagcc cgtcctcaac caggcttact gggtcgaact ggacaagctc tacgccgccc
     3781 acgacgtcac cttccactgg gtcaaaggcc acgtcggcgt cgaactcaac gagcgctgcg
     3841 accagctggc caaagcccag gccgtaaagt ataaataaaa acgaatcact agctatgagt
     3901 ggctagtgat tcgtttttac tgtaggggcg ccacgtgtgt caagaataac atcgtttaca
     3961 ttttgtgcaa ggacatcctt tacacttt
//
LOCUS       DAWABZ010000157         3967 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_5473_length_3967_cov_2.799591, whole genome shotgun sequence.
ACCESSION   DAWABZ010000157 DAWABZ010000000
VERSION     DAWABZ010000157.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5473_length_3967_cov_2.799591"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          839 a          956 c         1078 g         1094 t
ORIGIN      
        1 gcgggcggcc cgctacgcgc tgagggcttc ccgaagcgtc gttgtcagtt tgagtttgtc
       61 gcggcgaagc cgcatctgat tccgaggcga ccggcggcct gcgctcactt atttcccatg
      121 gggtgcataa ccgttgatgg cggccaggcg gatcagtttt tcaccttttt cgctgtcttt
      181 cgggacgccg atgccttctt tatacatctg gcccagccaa tattgggcca cggcggcttc
      241 gacgttgagt tcaccgccct gcaaggcttg ttcaaagtaa tggcgggctt tggcgtaatt
      301 ccgtgtgacg ccgcgtccca ggagatagag catccccagc atagcctggg cttctggcag
      361 tccctgttca gcggctttgg tgtaccaaaa ggcagctttt tcatcttttt tgtaactgta
      421 aaaataggca tcgcctaaat agttctgagc gtcccggtca ccctgggtgg ctgcattttc
      481 tatcagcatc cagcccactt tatcggcatc ccggacgtgg ccgccgtcag agaggagttc
      541 taagccccac gtatacaaat tgatggcctg attcttcttg gtctgttcgc ttctgtccat
      601 gactatccct cctgtgaaat acaatttata tcttcactat agcatctgag taggacaggt
      661 tttgtcatgg cgaggcaaaa agacgctgtc gcacgacgac agtgcctttt ttagcagtca
      721 gcggatggag ttaaatttaa aagcaacctc tcagtcagct tcgctgacag ctctcctaga
      781 aagagagcct ttcactggcc tgcgctccca ttagtccgct tctaagtgca gggttttgac
      841 cagtccgctg cggttatgct tgaccggttc gtcggtcagg gcatccagga tggggccgta
      901 gtttttcttt tcgatgtctt cgcagaaggc gattttcagg tacatcgtag cccgcgagga
      961 gccgacgtag atgaggcgct gcatgaggtc gtccgggagt tccgacagat tgacgtcgat
     1021 gaggaggacc gccgtggctt cgaggccctt gaatttccgt accgacgtga agaggatttt
     1081 gccgtcttcc ggttccgtcg tgacgggaat cccgttgagg gtccggcagc ctttgaggcc
     1141 gctatggccg acggagtggg ccgtgaggat gaccatgtct ttcagggcaa cgccttgctg
     1201 gcacatgtcc ttgacgaagc ggtctgcctg tttctgcagt tctttttcgt tcccataaaa
     1261 gacggctttc ggcgtcatgc cctggatggt gttgacataa ctgtctttca tgtgcatcaa
     1321 cttgccgatg gacgtggcga tttcagcggt attgcggcaa ttcttataga ggaccaggcg
     1381 acattcggca ttcttatcga tccagcccgt cgtcttgcgg ctcatgatgt actgattgcg
     1441 gtcgtaaaag atgtagaaac agccgtcctg taattctgcc aggaagctca ggtgttccag
     1501 gatagagtcg ctcaggtcct ggccttcgtc gacgacgatg ttgggatacg cccagtcatc
     1561 atcattgaat tcgctttcaa agtactgttc aaagacggca acgactttat tgaggggcaa
     1621 ggtgtcgtcc tggaggattt cttcagccaa agtgcgtaca ttatggaagg taatctgcgg
     1681 atcttgataa tggcgtcgca ggtactgcag gaggaattcg ttgaaacaca gatagagggc
     1741 cttttttccc tgactggcca gcatccgcgc tttttctacg gccagcatag tcttgcccgt
     1801 gccggccggg cccgtaaatg gccgccgtcc gctgttcctg caagaaccgg ggtatagaga
     1861 attgctggtt cgtcagctgg acgtaggacg tcttcgtttc ccggctgctg ctggaaatcg
     1921 tttcggcaat gcggaaagac ggcatgagtg tctggatgag ggctttgaat tccggcggct
     1981 tcaacggtgt aaaggaccgg cccaggtggt ctttccaata ggcataaacc ttgtccaggg
     2041 cttcttccgg atgtgctaaa ctcagctggt ccaggatgat ttccggaacg gcttcggctg
     2101 gcagacgcag tttcgaatcc atggcgaccg acgtgaacca ggccgcccgg ccaatcatgg
     2161 gacagtcata ggggaaatgc tggcgcagga tccgctggat acggtactgc gtttccgcag
     2221 cctgtcctaa gggattgatg cgttttttgc tgttattcct gcggttgatt tggatccaac
     2281 tgccttcttc atagccgata ccgccggatt tgacttcgat ggacaagatg cccttggtcg
     2341 ggtggaaaat gataaagtcg ccttcccctt cactccggta ttggcccggt tcgccgagcc
     2401 agcggaagga atggaagacg acatatccgt cacttaattg ttgtaaggcc tgatagacct
     2461 gtatttcgcc atcgctgcca tgaaaatcgt catcgatggt cggataaaaa cgtgccatgc
     2521 agtatctttc ctttctctgt atcttctgtc ttcgtgttct ccttattata ccagggatac
     2581 ttcagggacg gaagggtgcc gaattctttc agccgggctt tgagctgttt ttgaagtcca
     2641 tgttgccaga gataacggtc gatcatttct ttttgggcat acaacacgct gtcttcgact
     2701 tgatggcgcg tgaaggtatt caggccatcg atttctaaga ggcatgtatt caggcgtccc
     2761 accagttccc agcaggccat cttatcttcc gtgacggcct ggtactgttc tttcaattct
     2821 tcgcgcatat ggtaatagtc cgtcagcgaa tggacgttgg aaagtctgtc tttttccagg
     2881 acggcttctg ctttctcggc gacgccagcc cgttcgaagt cgtagaagtg gcagtcgttc
     2941 aggaggtagc catgggtgta ttttcccagt tgttcgttga aattcagggt aaagacggca
     3001 tggcagcgtt cacattcaac ggtctgtacc atattcagca gattatggat cagcaggttc
     3061 ccctgttcat cgcggtgatt acagacgggt tcccaggcat aacggcgccg gctttcctgc
     3121 aaggtcgtgc cggccttttt gaagcagtct tcgataagca tgagcgcttc gtccatattg
     3181 agccccggga tgtcgtcgag gagattgacc gtacaatcat aataatcgag agtattaaag
     3241 atgggcacgg aaaagctcag tacccaaaat gtcctgaatg tcatctgctg gtacagggga
     3301 aagaggatgc tgcggatgtg tttggctgaa aagtcatcgt aatagggcag gtccagggtg
     3361 atgtaatcca atttctctaa gatagccgtt tgtcgacgga tttcacgctg gagcagttcg
     3421 gcctggattt taaggttatt gtggaaaaag gtgacatctt tgggcgtgaa ctgctgcatc
     3481 ttgtccaaaa tatgattgga tttttcgagg tgtgctatga aatccggctt gagaaggtca
     3541 tccaggctgt tatatctaaa gacttcattg tagctgtatt cgtgcgtatc cgggtcaatt
     3601 ttcagcgaaa aaacagtatg gcattttttg cagatatacg tatcatgaca attcttggcg
     3661 ctatctatca tcgtcaggga gctggcatcc tcaaagagca gatgaccatc actgtcgaga
     3721 tgttcgcagc agtcgcgttc tttaaggacc gcccgacatt cgtccacgtc aagggcaaat
     3781 ttcttatagc agttatagat gaactggata tcttgctgtg cttgtactac gtcaatctgt
     3841 ttcattccta tcgcctcctt ggacatatta ttatacatca ataagaggac agaatgtgtc
     3901 ttagcataaa aaaaacttgt cgccgcgcga caagtttttt tagtggttag tgattagtgg
     3961 ttagtgg
//
LOCUS       DAWABZ010000158         3941 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_5519_length_3941_cov_3.132012, whole genome shotgun sequence.
ACCESSION   DAWABZ010000158 DAWABZ010000000
VERSION     DAWABZ010000158.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5519_length_3941_cov_3.132012"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          784 a         1027 c         1158 g          972 t
ORIGIN      
        1 cgagggtgtc ccgaagcgtc gttgtccacc tgagtctgtc gccgcgcagc ggcatctgat
       61 tccaaagcgg cctgcggacc gtccgtcgga acggccccta cgccaatttc ttgatgtcgc
      121 ccaagggctg gatgaggctg taggtcaggg ccaagaggtg gagacggttt tctttgaccg
      181 cttcgtcttt gtgcatgacc aggacgttgt ccaggaagtc attgatggcg gcaatgcctt
      241 cggagaggac ttcggctacg ccttcgtagt catagacggc gtatttctgc ggcaagttga
      301 cctgcatggc ctggcaggcg ctgtaaaggg ccttttcttc ttccgtttca aagaggtccg
      361 gattgacgtc ggtatcgtcg gcgcctttga tcatgttgct gacgcgggtg aaggcctgca
      421 agaggtcggt cttgcccatg atgtcggcat cgatgagggc ctgggcacgg cgggcttctt
      481 cataagcgtt gagcgtatcc gaagccagga cgcagtcgat gatgtggtaa tccatgcctt
      541 tatcctggaa gatgttcttc aggcgcagcg tgaagtagtc gtcgagctga cccatgactt
      601 tggctgcgtc ttccccttct acgccgagga gattgaggac gaaggcaaag acttcatggc
      661 agttgaggtt ccagccggca tccatgagga tgttgagggt accgatggtc tggcggcgca
      721 gggcaaaggg gtcctgcgag cccgtcggga tgaagccgcg gctgaacatg cccgtaatgg
      781 tatcgaactt gtcggcaatg cccaggactt tacccatgtc ggtctgcggc aggacgtcgc
      841 cggcaaagcg cggctggtac tgttcgttga tggcttcggc cacgccagca tcttcgccgt
      901 cgatgagggc atattccttg cccatgacgc cctggagttc cgtgaattcc atgaccatct
      961 gcgtagccag gtcggctttg gccaggagcg atgcgcgctg cagcttggcg tcatccaggc
     1021 cgaggtcgag tttatgattg aggaagaccg taatcttttc caggcgctgg gctttgtcat
     1081 agagcgtgcc caagccgtcc tggaagacga ttttcttcaa tttttccgtg taatcggcga
     1141 gcttatgttt cttgtcttct tcaaagaaga acttggcatc gtccaggcgg gcgcggagga
     1201 cgcgttcgtt gccgtgctgg accgtttcca gatggtagtc gttgccgttg cggacagtca
     1261 ggaagaggtt catgagcttg ccgtctttat cgcgcatagg gaaatagcgc tggtggtcct
     1321 tcatcggcgt gatgatggcc gcttccggca gtttcaggta gtcttcatcg aattcgccgc
     1381 aaagggccgt cgggtattcg acgaggtaga cgacttcttc caggaggtcg tcgtccatga
     1441 tgatggtgcc gcccttttcg tcagccagtt tctgcagacc ttcgaggatc atctgcttgc
     1501 ggacttccgg gtcgacgatg aggaaatgtt ccttgcacgt ttcttcatag gctgccgggc
     1561 tttcgatggt gaaatcgccg gtacccagga agcggtggcc gcggctgacg ttgcccgatt
     1621 tgacgttagc cagttcaaag gggatgacat ctttatcgaa gagggcgacg aaccagcgga
     1681 taggacggac gaagtggaag tccaggctgc cccagtgcat gctcttgggg aaattaagac
     1741 ccgtgacgat gcccttcatg aggtccggca agaggccggc tgtgtcagcg ccgacgctgg
     1801 tgacgttggc atagacgtag ccgtcttcaa cgacgaggtc cttgacgtcg attttctgac
     1861 cccgggcaaa gcccatggca gctttcgtcg ggttaccgtc ggcatcgtaa gcgatcttga
     1921 ccgacgggcc tttatgtttg gccgatacgt cggcctgttt ttcagcgacg cccttcatga
     1981 tgagggcgat gcggcgcggc gtaccgaggg tgcggatcga ttcataggcg atatgggatt
     2041 cgccaaagga tttcccggcc aggtcccgga cctgggaaag gatactcggc atataatgag
     2101 ccgggatttc ttcagtaccg atttcaagta acaaatcttt gctcatctta tttttcctcc
     2161 ttattcagca tcgggaaacc taatttttca cgctgttcca agtacgcttt ggcgcaggcc
     2221 cgtgccatgt tgcggacgcg gccgatgaag gcagcccgtt cgctgacgct gatggcgccg
     2281 cgggcgtcga ggaggttgaa cgtgtgcgag catttcaata cgtagtcata ggccggaagg
     2341 acgaagccgg ctttgatgac ccgcatggct tctttttcgt attcatcgaa gaggtggaac
     2401 aagaggtcgg cgttgctcaa ttcaaaggca tacgtcgact gttcgacttc gttctgatgg
     2461 aagatgtcgc cataagtgac gtcttcattc catttgaggt catagacgtt ttcgacgccc
     2521 tggatataca tggccaggcg ttccataccg tatgtgattt ccgacgaaac aggagcgacg
     2581 tcgatgccgc cgacctgctg gaagtaggtg aactgcgtga cttccatgcc gtcgagccag
     2641 acttcccagc ccaggcccca ggcgccgagg gtcggcgatt cccagttgtc ttcgacgaaa
     2701 cggatatcgt gttctgccgg gttgatgccc aacgtttcca gactcttcag atagagttcc
     2761 tggatgttgt ccggcgacgg tttgcaaatg acctggaact gatgatgctg gtacaggcgg
     2821 ttcgggttgt cgccatagcg gccgtcagcc gggcggcgag acggttcgac ataggcaacg
     2881 tgccacggtt ccggcccgag cgtgcgcagg aaggtagccg ggttcatggt ccctgcccct
     2941 ttttctacat catacggttc gtagatgata cagccctgag aagaccaaaa gctcttcaat
     3001 gcaaatatca tgtcttgaaa attcatcgta ttccctccta aatacaaaaa agtccctgta
     3061 tcacatgaca tgttacaggg acgagtatac gtacccgcgg ttccaccctg attggtccaa
     3121 agacccgctt gattcatcgg ccatacacat cgaaaagact tccggccgcg gccttcagag
     3181 cgccttccgc atcggcagca ggctcacact gtccctgctt cgctgaggat gatacgtact
     3241 tactctcctg catcgcctat tgatttgtac aaaccttatc atagcagaga tggggggaaa
     3301 agtcaataaa aaggagccgt ctcgtagaga cagctccttt tgagtttgca gtttgtagtt
     3361 tgcagtaatc aatactttga atggattcac tacaaactct atactaaaaa ctttaaactc
     3421 actatcattg cttgccgtat ttttcggcga cgtccaggcg gcgcagggct cgttgcaggg
     3481 ccgcttcggc gcgggtcacg tcgaggtcgg cagcgtgtgc aaccaagcgg tcttcggcac
     3541 gtttttccgc cgattgggcg cgggctacgt cgatgtcttc cggtttttct gaagctggcg
     3601 tgatgactgt gaccgtattg tcatggactt ccaggaagcc cgacgctacg gtgacgcact
     3661 ggcgctttcc gcctttatca tagcacaggg gccaaatcga cagggacgcg atgagcggcg
     3721 catgattggg catgatgccc aagtcgccgt ccaatgcgcg gacgaggacg aaatccacat
     3781 cttcatggag tacggcagca tcgggtgtaa tgacttccag atggagggtc tttcctgctt
     3841 ctgccataga ctattcccct ttcagtgtct tagccttttc gtatgcttct tcgatattgc
     3901 ctaccatgta gaaggcctgt tccggcatgt cgtcgcattt a
//
LOCUS       DAWABZ010000159         3937 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_5530_length_3937_cov_3.659454, whole genome shotgun sequence.
ACCESSION   DAWABZ010000159 DAWABZ010000000
VERSION     DAWABZ010000159.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5530_length_3937_cov_3.659454"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          940 a         1056 c         1102 g          839 t
ORIGIN      
        1 gtgtaagggg cttttggaca gccctttgag aataaggcta tcctgatttc aggagaaaac
       61 ttaaacagca accttctcaa gaatctcccg cgtctataag cgggagaggt tcaaaaaacc
      121 tgccatcatg ccagagtgag ctgtggtgga atttcgcggc gggccgcccg tcgggacggc
      181 ccctacgaaa aggccccgat tggggcgatt tttacgagcc gagggttact cgaggcgatg
      241 tcgttattct gagtctgttg ccgcgtagcg gcatctgagt ccgaggcggc ctgcgggcgc
      301 tttagcgccc taactacttg cggaattgcc tattctacgg taatataaac gtatacaata
      361 ccttattata tacacaggag gctgttacta tggcagacaa taaaacatac gctgtgttcc
      421 agacgaaccg cggcgatttt acggtagaac tttttgcaga caaggcaccg attacggtcg
      481 aaaattttgt gaatttggca aaatccggtt tttatgatgg cacgattttc catcgcatca
      541 ttgccgattt catgatccaa ggcggcgacc cgacgggtac gggatgcggc ggctcgaaac
      601 agacgattcc tgatgaattc ggtccgggcc tcgatttcag cgaacccggt gtcctggcta
      661 tggccaatgc cggccctaat acgggcagtt cccagttctt cgtgacggtc gtcccgacgc
      721 cgtggctcca gaaccatcat tctattttcg gcaaggtcgt caaggattat gacgtcgtcg
      781 aaggcatcag cagagtcaag accgattttg ccgataaacc gctggaagat gttgtcctga
      841 atacggtgaa aatcgtcacg gaagaagcct aagggcttca tgttatatta tacgtatatg
      901 gtgcgctgcg ccgaccagtc tttatatacg ggctggacgc tggatgtgcc ccggcgggtc
      961 gcagcgcata atgacggcag aggggctaag tatacccgtt cccgtcgccc tgtaaccctg
     1021 gcctggtgcc agggttttcc tacaaaacac gaggccatgc attgggaatg gcaaatcaag
     1081 cagtggacga aaaagaagaa ggagaagctc tgccgggaac agctttcgct aacggatgag
     1141 ggaggatgat agggatgaaa ttatggaaaa aatggctgac gactctcgtt gccagcctgt
     1201 ctctctgcgt ggcgcctgtc gccatcgctt cggctgcgtc gacggcgcag atcctgctct
     1261 atggggcagc gactatggct ctggccaaac agcagctgct ccagatggac aacaacaatc
     1321 agaagcagat gttggccaat acgcaggcca agactggtgt cgtcgataat gaagcctaca
     1381 gcgaccgtct ggaaaatatc cagcgcaacc tcgtagcgac gggcctcatc aagcgcaatt
     1441 acgatgtcta tgccaacccg tcgaaagagt tgaacgcctt tgaaacgatt ggcggcgtca
     1501 tttccgtcaa taaggggatg ctcgatgccc tcaacgacga tgaactggcc tttaccatgt
     1561 gccatgaaat gcagcacggc gaaaaacgcc atgccatcaa cggcgtcctc aagagtatcg
     1621 gtatttcgac gctcatcgac gtttcccttg gcggtaatgc cgacgtcctc gacattctcc
     1681 tgggcagtgt ggccgtcaat tacatcgaca atgaattcgt cactatggac caggaaaaac
     1741 aggccgatgc gacgggcttc aatgtcttca agaacacggc ctacaatgtc ggcggcgcgg
     1801 cttcgtccat gcagtacgtt tacgaacagt acggcgaact gtggcaggaa ggcttcaagc
     1861 gcgtcatcag tccgaataat catcctcaga tgtcgagccg tattgaaaag ctggccggcc
     1921 gcatgacctt gtggtcgggc aaccgcgtcc aggtcggcgg caacacggtc tatgtcaatg
     1981 ccaaacccgt cgtcacaccg gctgcagccg ggacgtattc gtcgcggcgc cgggctttcc
     2041 tggtagcggg caatctggcc cgggctacgc atgacgtcta cggcgaagcg acgaaagacg
     2101 acaaggtcgt tacggaagcg atgaacaaga agcagccgtc gtggaacgta gcgtcccgcg
     2161 gtaacgatgt ctatgtcaac gacttgcgca tcatgacctg tgctgacggt gatgatcagc
     2221 cggctatcgt caagaatatc cagtcggccc tggaagccaa gccggccatg ttgagcaaga
     2281 aggaaatcca gaagcagaat aaagcctggt cccagaagta cggttacaag aaagataaga
     2341 aggagaagaa tgcgtaaaca attagctttt atcacggctc tcatggccct gaccctgggg
     2401 gtcggcatct ttgccggcgc ccgtcagtcg acggactccc acggtgtcga cgtggccctg
     2461 gaagaagcgc ggcagcatgt aaaggccgat agtgatgaca tgccggcttt gcgggaaaaa
     2521 ccgcatgtca ctctccatta tccggcgacg gatccggctg atggccatcc caccctgcgc
     2581 tggagccgtg tcgacggggc tgtcatgtat gacgtgcaga tcttgaagaa agaggaagaa
     2641 aaaggcgatg acggaaaggt cagtacgtct tacgagcctt tcatggatga ccagaaggct
     2701 tatacgacgg gactggaact ggatttgccc gataccttcc tgggacaggt cttttactgg
     2761 cgcgtccgcg gcctcgattt gaagggccgt ccggtcagtg aattttcgga tatcgaagaa
     2821 gcgcatgtcg atattttcca gccttttacg gaaaagccga cgcccttgtc ctttttcaac
     2881 cagggaccgg gacagaccct gctctatccg gtctacgact ggatagccgt accgggggca
     2941 gcaaagtatg aagtagaaat cctcaacgat ttgccggaag atccgaatgg tacggcgccg
     3001 tcgattcatc gcatcgacag ttacacgccg caatatgccc agcagtacga ccagaaggcc
     3061 cgcatgggcg ataagccctt ttactggcgc gtcctggctc tggatggggc aggcaatcct
     3121 atcggcggtt attccgatgc cctgcccttt gagctggacc cggcagcgga acagggcgtc
     3181 gtcgctatct ttggcgacag tatcagccac ggtggcggca gtatttccta ttcgccgacg
     3241 gattgggact tcagttacgc ttcgtatctc atgttcccga cgatcaacct ggcccagagc
     3301 ggcgatacga gcgccatggg cgtcgaacgc ttcgaccgcg acgtcctgcc cttctagccg
     3361 tcgtatgtca ttatcctcat cggctccaac agcctccgcg ccggcgtgcc ggccggtgaa
     3421 gtcatcagtg acttacagac gttgaaggaa aaatgcctga gccatggcat caagcccgtt
     3481 ttcctgacca ttccgccgct caatccggag aacatcaagg ctgccttcga ccaggatacg
     3541 gccgatgact ggcgcggcgc cattgccgaa gtcaacgact acatcgacac gcaggtccat
     3601 atcgacatca cgccgggcat ggccgacagc cgcggcgaac tcaagaccga actggccctg
     3661 gacggactcc atctcgaccc gccgggcaaa aaaatgatgg ctgctgccat caataacgcc
     3721 tgggccagca tcaccgccct gcccgatagc gcctgggagt gaagggcggg cacatgaagg
     3781 ggtctgccat catgcgaggc cccgttctct ttatagtttg tagtacggag tttgtagttt
     3841 gtagaagttg actttaaatt tttagccata tttctgcaaa ctaaaaacta caaacttaaa
     3901 acttaaaaag ggcctgtcgc acgtaatcgt gcacagg
//
LOCUS       DAWABZ010000160         3892 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_5594_length_3892_cov_3.025541, whole genome shotgun sequence.
ACCESSION   DAWABZ010000160 DAWABZ010000000
VERSION     DAWABZ010000160.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5594_length_3892_cov_3.025541"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1004 a         1060 c          942 g          886 t
ORIGIN      
        1 ctcgccggca tcgtcaccga tgcggatttg ctctacaaga aggtcaaacc tcatgtccct
       61 cactatgtca atcttttggg agccagcatt tattataacg gcatcagcga atacgacaag
      121 gggttcaaga aactcatggc ctgcacggcg aaagatatga tgaccaaaga cgtcatcatt
      181 gccgcaccgg atgcggaagt cgaacagatc gccggcgtca tggtggccga acatctgaag
      241 gtcattccta ttgttaaaga aaagcacatc gtcggcatcg tcacccgcgg caacatcctc
      301 gatgaattgt accgtgaata cggcgacgac taggggaaag ggggaataga caatggctga
      361 aaacatcaat aaaggtccgg gaccgggcga agacaaggaa gcggaacgcc ctgtcgtccg
      421 cgtcaaagat gtcatggaaa aggttttcgt ctcggtgaca ccggatattc ccatccgcga
      481 cgtcttccgc atcttcatca agcatcgcct gctggctgtg cccgtcgtcg atgaagaaga
      541 ccacctcatc ggcctcattt ccagcgccga cctcatgtcc cgcagttcga agccacacat
      601 tccgcgcctg cccttcatcg gcaccaaaat ttataccagc cgcgtcggca aatacgccaa
      661 ggaattcaag aacctcctgg atcagccctg cgaaaaattg atgaccaaga acgtcatgat
      721 cgcgacgccc aaggccgatg tcgaacaggt cgcagccgtc atgatcctgg aacacttgaa
      781 ggttcttcct gtcgtggaca acaagcgcgt cgtcggcatg atcacccggg cctgcatcct
      841 caaagatttg taccgggaat ggaagtatta gacctttgtg actagtcact ctctgcctcg
      901 tgtgctataa tattataatc gaatgattta ggcagcggcc gctgccgcag aaagtatttc
      961 acgaggaggc acttaatgga taccaaagaa ctcgtcatcg ttgtcgattt tggtggtcag
     1021 tataaccagc tcattgcacg gcgcgtccgt gaaaaccacg tatactgtga agtatacccg
     1081 tatcacaaag cagttgcaaa gattaaagaa cttcagcccc agggcatcat ctttaccggt
     1141 ggtcctggca gcgtctatga agataatgcg ccgaagatcg aagaagaagt cttccatctc
     1201 ggcatccccg tcctcggcct ctgctatggc atgcagttca tggcccagac cttaggcggc
     1261 gaagtcaaac acgccgacat gcgcgaattc ggcaagaccg aaatcgatgt caatacggct
     1321 tcgcctttgt tcaaaggcct gcaggaaaaa gaaatcgtct ggatgagcca tcaggactac
     1381 gtcgcttcca tcccggaagg ctttgaaatc gtagcccaca cggccaactg cccggtcgct
     1441 gccatgcaga acccggctaa gaagctctat gccatgcagt accatccgga agtgctccat
     1501 acggaacacg gcaaggaaat gctccataac ttcctctttg aagtctgcgg ctgcaccggt
     1561 tcgtggacta tggccaacta tgccaagacg gctattgcca atatcaaaga aaccgtcggc
     1621 gacggcaaag tcgtcctggc cctttccggc ggcgtcgaca gctctgtcgc agcagccctc
     1681 atctccaaag ccgtcggcag ccagctgacc tgtatcttcg tcgaccacgg cctgctccgc
     1741 aagaacgaag gcgacgaagt cgaagcggcc ttcaaggaca gcggcatgaa cttcatccgc
     1801 gtcgatgcgt cggaacgctt cctgaagaaa ctcgaaggcg tcagcgaccc tgaacggaaa
     1861 cggaaaatca tcggcgaaga attcatccgc gtctttgaag atgaaggccg taaaatcggt
     1921 tccgtcgatt tcctggccca ggggaccatt tatcccgacg tcattgaatc cggtgccggc
     1981 gatgcagccg tcatcaagag ccatcacaac gtaggcggac ttccggctgt cgtcgatttc
     2041 aaaggcctca tcgaaccgct ccgcaacctc ttcaaagacg aagtccgcga actcggtgcc
     2101 gaactgggct tgccagaata cctcgtatgg cgccagcctt tcccgggacc gggcctggct
     2161 atccgcgtca tgggtgaaat caccaaagaa aaactggcca tcctccgcga tgccgatgcc
     2221 atcttccgcg aagaaatcac caatgccggc atgcagcgcg atatcaacca gtacttcgcc
     2281 gtcctgacca ataaccactc tgtcggcgtc atgggcgact tccgtaccta tgactacacc
     2341 ttggccctgc gcggcgtcac cacgaccgac ttcatgacgg ctgactgggc gcgtatccca
     2401 tacgacgtcc tcgataaaat ttccagccgc atcgtaaacg aagtagacca catcaaccgc
     2461 atcgtgtacg acatcaccag taaaccaccc gcaacgattg aatgggaata gttgcaaatt
     2521 gcctcacaaa tcgcttaacc aagccgtttg tgaggctttc tttttttcaa aagttccaaa
     2581 agtgtaccag aaaaatttaa aaaacctttt ttagggccta tttttgccaa atttctcgaa
     2641 caaaaaatgt gcatttttat acgtataaaa caaaagcggt tacaaaagcc gagtagattt
     2701 gcaatgtaaa aatatacacg gccaacggcc ttgtgcgtag gagccgaccc ggacagggtg
     2761 gcctgctgcg atatttaaca acaccttgtt ctaacattat ggagagcctt tacgaatcac
     2821 gaacaccgag ctgcgtgttt ttcctcggtt tcatatgcgg cttcgccgcg agatactcaa
     2881 atcgtattac gaacatgtgc cactcgtcat aaattacggc tacgtacagg attcacgcgg
     2941 gctcgccacg tgtgagcccc tacgatgcgg cttcgccgcg acgaacttat gattgtatta
     3001 cgaccatgtg ccattcgtta taaattccgg ctatgcacaa aattcacgcg gcctttttgg
     3061 cccgtcaggc gccgaccata agaaggcgca ggacggttgc caaaacggac agctgtggct
     3121 gcccgccttt atcaggacgg cccctacaga atcgcagcta ggcgataaat ggaaacaacg
     3181 ttcaccggac ataaaatata cacggccaac ggccttgtgc gtaggggccg acccggacag
     3241 ggcggcccac tgtgagattt taccgtagcc tgttctgata taatggcagg tctttatgaa
     3301 tttgaaatgg acaaagcaaa caggcgctgt cttcgcgtga cagcgcccgt tatactagat
     3361 ttttgatgat tcaatttttc aagtagacgg caatgtctgc ctgtgatata ctgaatccga
     3421 ggttttgcct aaaacggtag gcggttggtc ccttgctttt cccggaggga ctttgtgtgc
     3481 ccctccaatc caactggaaa ggaggggagg acaatgactt taacggtgga tctgtttatc
     3541 agtatactat ctttcggtat ggccgcattt gcaattggtt atgccctagg tcagagttca
     3601 aataaaacac aaaaatagcc gccagagacc cccatcttaa gcgactattt ttgttgactt
     3661 atcggggcca accgtctatc ggcaggacct cttttcatgt ctattataac gtcatttatt
     3721 tatcttcgtc aagcggatac tatgtgatgg atttaggcag ttagccttta ctgtacagtt
     3781 gtggagtcgg actcatatgc ggcttagccg cgagatactc aaatcgtatt acgaacatgt
     3841 accattcgtc ataaattccg gctatgcaca aaattcacgc ggcctttttg gc
//
LOCUS       DAWABZ010000161         3872 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_5625_length_3872_cov_2.984281, whole genome shotgun sequence.
ACCESSION   DAWABZ010000161 DAWABZ010000000
VERSION     DAWABZ010000161.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5625_length_3872_cov_2.984281"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    649..658
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          969 a         1149 c          958 g          786 t
ORIGIN      
        1 cctgggcatc gcctgcggcg tcggcatcgc cctggctatc gccaagtcgg gcctcttcca
       61 gacagtcatc acggcagcac cgatcatcat ctccttcagc ttctccgtcg gcatcggcct
      121 cttcttcggc atctacccgg cccggaaagc agccaaactc gaccccatcg aagccttgcg
      181 gtatgaataa aaaaaatgcg ctgtcgcatg aagacagcgc attttgagta ttaagtttgc
      241 agtttttagt ttgcagcaaa tggtaaaaaa tttaaaccca cttctacaaa ctccgtacta
      301 caaactataa agtaaaaggt gccttgcacg atggcaaaaa tcttcgtgcg acagcccctt
      361 tttagttgtt cgtgattcgt ggttcgcagg ggatgtgccc aaagcttttt tggcccgtca
      421 ggtgccgacc ctcggaaggc acctgacggt tgccagaacg gacagctgta gcggacgtcc
      481 cggttctcct gccaggagag ctgtcagcgc agctgactga gaggttgctt ttgaatcgtc
      541 gtacggttca tgggattctt ataggagagt ctggaaattc aacctctccg gcccgttggg
      601 ccacctcccc tatcatggga ggcttggcgc cctgcaatcg gcgggccgnn nnnnnnnncc
      661 cctacgaacc cctagccacg agccacgaac cacgagccac tagccacaaa ccacgagcca
      721 ctaatctctc agccagcggc ggatgtcgtc ttcggcatct tcgatggtcg tacccaggag
      781 ggccaggctc ttgccgagtt tggccgtcgg gcagagagcg tggatgtctt ccaggctgtg
      841 accgacgccg ctgccttcat gggtgcagaa aggataaatc gtcttatccg tgaagtcgtg
      901 gctttccagg aacgtccaca aggccatggg catggtgccg caccagttgg gatagcccag
      961 gaagatgacg tcgtaatcat cgatgctgtc gatgtccctt tccaggaccg gccgggcatt
     1021 gtcggccttt tctttcttgg caatcttgac gacttcctga tagtcattgg gatagaccga
     1081 agccgtgcgg atgtggaaga ggtcgccgcc cgtgaacagg cgcagcatct gtgccgcccg
     1141 ttccgtattg cctacgggca ggtcgacgat cttaccgctg acatagttca tgccggcgtg
     1201 tgaaaaatat gcaatgaggc atttcttacc ttgataatcc atcagtcaat caaacctccc
     1261 tgatatgttt gacttaaaaa ttcccgatac agttttcatt atacggcaaa aatcgttgcc
     1321 attcaatcac tatcatgaat gacggccatt ttattttatc atgcctgccc ggcgcccagt
     1381 atctttccaa ggcccatata atgtagtaaa atagagtttg tcaatagatt actatgctaa
     1441 gcaatacaag gaggtccctt tatggctcat gtaaatgaaa actacttaaa actccccggc
     1501 aactatcttt ttgcaaccat tgcaaaaaaa gttgaagctt attccaaagc acatccggaa
     1561 gccaatatca tccgcctcgg catcggcgac gtcacccgtc ccctggcccc ggctatcatc
     1621 gacgccatgc acaaggccgt cgacgaaatg ggcaaagccg aaaccttccg cggctacggt
     1681 ccggaacagg gctatgactt cctccgtcag gctatcatcg acggcgacta caagacgcgc
     1741 ggcatcgacc tcgaattaga cgaagtcttc gtcggtgacg gcgccaagac cgatgtcgcc
     1801 tgcatccagg aaatcttcgg cgacgacctc aaattcgccg tagccgatcc ggtctacccg
     1861 gtctatctcg actcgaacgt catgttcggc cacacaggcg actggaatgc cgaaaaaggc
     1921 atctacgacg gcgtcgtcta tctcccgtgc accccggaaa acggcttcaa agccgaaccg
     1981 ccgaaggaaa aagtcgacat cgtctacctc tgcaacccga ctggcacggc catgagcaaa
     2041 gaagaattga ccaactgggt caaagctgcc aaagaaaaca acttcatcat catctatgat
     2101 tcggcttatg aaacgtacat caccgaaccg gacgttcccc acagcatctt tgaaatcgaa
     2161 ggcgctaaag aagtagccat cgaactccgc tcctactcca agtgcgccgg cttcacgggt
     2221 acgcgctgct cctatgtcgt cgtcccccat gcctgcgtcg cttacaagaa agacggcacg
     2281 gctgtcgaac tcaatccctt gtggaaccgc cgtcagtgca ccttcttcaa cggcacaccg
     2341 tacatcgtcc agcgcgcagc tgaagcttac tacagcccgg aaggccagaa acagtgcctg
     2401 gctgacgtcg aatactacat ggaaaacgcc cacatcatcc gcgacggcct gaccgaagcc
     2461 ggcttcaccg tctacggcgc taccaactcg ccgtacgcct gggtacagac gccgaacggc
     2521 atgaagagct gggatttctt cgacctgctc ctggaaaaag cccacgtcgt cacgacaccg
     2581 ggttcgggct ttggccccca cggcgaaggc tatctccgcc tgaccgcttt cggcaccaaa
     2641 gaaaacaccg tcgaagctgt caaacgcatt gctgatttaa aattattcaa ataaaggtgg
     2701 tttaaccatt gagtaccaat ctggaagtag gcatcgtcgg cctgccgaat gtcggtaaga
     2761 gcacgctgtt caacgcgatt accaaggccg gcgcagaagc ggcaaactat cccttctgca
     2821 cgattgaacc gaatgtcggt gtcgtagaag tcccggataa acgcatccag gtcctgaccg
     2881 acatgtacaa accgaaaaaa acaattcccg ctgtcatgcg cttcgtcgat atcgccggcc
     2941 tcgtcgccgg tgcctccaag ggcgaaggcc tgggcaacaa gttcctcagc cacatccgtg
     3001 aaaccgatgc catcgctgaa gtcgtccgct gctttgaaga ctcgaacatc acccacgtcg
     3061 acggctccat cgacccgatc cgcgacatcg aaacgatcaa tacggaactg tgcctggccg
     3121 acctggaatc ggcccagaaa cgggcggacc gcatcgccaa agtcgctcag tgtggcgaca
     3181 aggacgccaa agtcgaatac gccgtcctga aaaaagtcct ggcctgcctg gaaaacggcg
     3241 acccggcccg caaagccggc ctgacaaaag atgaactgcc gcaggtcaaa gaactcaacc
     3301 tcctgacctt gaagccgatc atctacatcg ccaacgtcgc agaagacgaa gcaggcaatc
     3361 ccgatgccaa ccccaacgtc aaacgcgtcg aagaatacgc cgccaaggaa ggcgctcagg
     3421 tcgtcgccgt ctcggccaag attgaagccg aaatcgccga actgccggac gacgaagccg
     3481 aagtcttcct ggaagaactg ggcctgcccg aatcgggcct ggacaagctg atcaaagcca
     3541 gctattccct gttgggtctg atcaacttct tcacggccgg atctgacgaa gtccgcgcct
     3601 ggaccatcgt caacggcacg aaagcccaga aagcagccgg taagatccac accgacatcg
     3661 aacgcggctt catccgcgcc gaaatcgttt cctacgacga cctcatcgcc tgcggcagcg
     3721 aacaggctgc caaagaaaaa ggccttgttc gtctggaagg gaaagattac gtcatgcaag
     3781 acggagatgt cacatacttc cgctttaatg tataggaaaa aaaggggctg taacataata
     3841 gcctcatcga aaaaaatgga ctcagttatc tg
//
LOCUS       DAWABZ010000162         3728 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_5859_length_3728_cov_2.395045, whole genome shotgun sequence.
ACCESSION   DAWABZ010000162 DAWABZ010000000
VERSION     DAWABZ010000162.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5859_length_3728_cov_2.395045"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1738..1747
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          766 a          952 c         1068 g          932 t
ORIGIN      
        1 ggacaatgcc gttggcatga gcattttcca gggcttcgcc gatatcgaag gcaatctgta
       61 tcgacgtgtt gatcggcagg tggccgtgtt tttcgatgta ttcttttaac gtttcgccgc
      121 gcacgaattc catgacgatg taatggacat tttcatcgta gccgacgtcg tatatattga
      181 caatattggg gtgtgacaat ttggcagccg cctgggcttc ctggcggaag cggacgacaa
      241 aatcgtggtc gttgccgtat tttgaatgaa gtatttttac ggcgacgatc cggtccagga
      301 ggatatcctg tgctttgtac acgctggcca tgccgcctgt gcctactttt tccaatatct
      361 tgtatctgtt atctaagata tgaccaatca tttttagcta ttccaccact ttcatagtcg
      421 ttcccgatta tatggttgtt aaaattgccg tgatattatc tttcgccccg ttttcataga
      481 cgcgggcaaa gagatcattg atgatttccc gttctgtccg gcggtaatcg ccgatagcat
      541 cgtagagttc ctgtttctgg atgaatgatg tcaagccgtc ggaacaaatc aggatgcgat
      601 ctttcggctg tacttcaaag gctccgctgt cgacgaccaa gttctcatcg acgccgacgg
      661 ctctggtcag cacatggcgc tggggatgat ccatcgcttc ttcttcagtt atcttgccct
      721 tttcgagcag gacctggacg atcgtatggt cgacggaaat ctgtttgaat tccccgtcgc
      781 ggtataagta gatgcggctg tcaccgacgt ggccccagaa ggccatatga cgagccaggg
      841 tcaccatgga cacggtcgtc cccatgccct tgagcgaagg gtcgatcaag gtcttgctga
      901 ggatgctgga attcgcatat tgaatggctt tctccagctg taccggtgct gcataactaa
      961 agttcttgaa ataataagct atcgcttcta cggccgtctt gctggcgatt tcaccgccta
     1021 catagccgcc cataccgtcg gctacggcca gtacgttttt ctcgtggctg atataaaagg
     1081 aatcttcatt cactttccga accaggccga ttttggattc accgtatgtg cccaaattct
     1141 cacgtcctct cacggtgttt cgttataggt acacattaat tcatatcata ttcgccgtca
     1201 cctgtcaaat tttttcgcgt tttatgcgca gctggccgca ggcggcctgt atctggctgc
     1261 ccatttcccg ccgcagagtg acggaaacgc cgtggcgctg caaatacgtg acgaattcat
     1321 tcatcgtctt ttcatcgggc cggtagaggc cgacgtcgta gttgtcgttg atgggaatag
     1381 cattgaccag gatgttctga ccgcccagga gacgggccag ttccttggcg caggcgttgg
     1441 tacaagtcag gcccttgatg aggatgtatt caaaggtgac cttccggcca gtgacgtcga
     1501 aataatggcg ggccgccttc aggacttcgt cgaggggata tttctcggca atcggcatga
     1561 tgcgacgccg caaggcatcg ttcggcgcgt ggagggatac ggccagggtg atgggcatgc
     1621 cctctttggc caggtcgtac atcttgggga cgatgcccga cgtcgacaag gccatgttgc
     1681 gatagcccag atagagcgta tccttttcgt ggatgagctt catgaagcgg atgacgtnnn
     1741 nnnnnnncgt tatcgtagtt gaggagcggt tcccctgtcc ccatgatgac gatagaatgg
     1801 aggtccggcg tgacgtattt gcggaaagcc aggagctggg cgaccatttc gccagccgac
     1861 aggttgcgga cgaagccatt cttggcggac gcacagaagg cgcagttgac ggcacagccg
     1921 acctgggacg atacacagat ggaattgcca tagtcgtgct tcatgaggac cgtttctacg
     1981 gtctcaccgt cagccagctg caataagagc ttcactgtct gtccgtccgg ggcgtccagg
     2041 cgggacacga cttccgggaa atagattggc agcacttcct tcagctgttc ccggtctttc
     2101 ttgggcagta ggaccatatc attccaggaa aagatatttt gcttataaat ataatgaaaa
     2161 atctggtcgg cccggaactt tttcatcccc tcggcgacga ggaacgcctg gagttccttt
     2221 ttggtcatat caaatacatt actcatgtca gccctccttt ttcatgcggg caatgaagaa
     2281 accatccagg ccgtcgcgct gtggcaggag ctggatgaaa ggcccttcat ggccgggaac
     2341 gggaagccac tgcgtcgcgt tttctacgtg gaattccgga tgattcttca aaaaggcagc
     2401 cacgatgcgg ctgttttcat catcattcat ggtgcaggta ctgtagacca ggatgccgcc
     2461 aggacggacg cattgggatg cactgtccag gatgcgccgc tgcagggccg gcagggtgcg
     2521 caggtcactg ggattcttgc gccagcgcag gtcgatctta tggcgcagga cgccgaggcc
     2581 cgaacaaggg gcatcgacca ggacccggtc ggctttgccg gcgtatttct ggccgatggt
     2641 gcagccgttc tggagcaggg tccgcacatt cttgaggccc agtttctggg cattatattc
     2701 gatgagccgc agtttatgct catggatatc gccgccgtag acgacacagg acgggccgcc
     2761 cagtgtggcc aggtgcgtcg tcttgccgcc gggagcggcg cagacgtcga agatgacttc
     2821 gtgcggctgg ggatcgacga tatgggctac cagctgggac ggttcgtcct ggatgatggc
     2881 ccggccatca cgcaggcatg tcagttcatg gatgccggga ttgccgtcga ggtagatgcc
     2941 gtctggtgaa atggcagccg ggcgggccgg cagaccttgt ttttccaaat cggccagcag
     3001 ctcttcgcgg ctggtctggg ccgtattgac gcgcaggcac agcggcggga tcgtatcgaa
     3061 atattggcag agccgttccg tatcgtccgg gccataggct tcgatccagc ggtggacgag
     3121 ccattcctgc tgatgatagg tcagggccag gtgggctacg atgttttcgc ccttatcggg
     3181 gatgacgaac tggctgcggc ggcggatact gttgcgcagc atggcattga cgaacttggc
     3241 catgccccgg ttgccgaatt tcgtcgccat cttgacggct tcattgcagg ccgctgattt
     3301 ggggactttg gtcatgccga aaatctgata taagcccagg cggatgatgg ccaggcagac
     3361 agggtcgagt ttttgcagtt tgcgcgtact gatctgtgat atgatccaat ccagataatt
     3421 caggcagcgc aaggtgccgt aaaccagctc cgtatagaag cgccggtccc tgtcggcaaa
     3481 ttgatgggtc tgtatcgcct tggagacgac gatattgcta tagccttcat cgcggcagat
     3541 agtcagcagg ctctgaaagg caatttcacg aacgttcatg gattcctccc tgtgctttta
     3601 aagcagtagc gaacagcaca aaagcagggc aaccgtcttc gttgcggcag ttgtcccgct
     3661 tctctgcgct gagtcattta taataattca ccagccgtca aggctgcgct ttgcagatat
     3721 tgggcagc
//
LOCUS       DAWABZ010000163         3711 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_5892_length_3711_cov_3.271061, whole genome shotgun sequence.
ACCESSION   DAWABZ010000163 DAWABZ010000000
VERSION     DAWABZ010000163.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5892_length_3711_cov_3.271061"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          712 a         1028 c         1131 g          840 t
ORIGIN      
        1 gccgtagcgg gcgtcccgcg tgaatcctgt gtacatcccc aatgacatac gcggcaataa
       61 actcagaata acgacatcgc ctcgagtgac cctcggctcg tgaaaaaata gtggctcgtt
      121 gttcgtggtt cctggctcgt aaaagcctga cattatactc ggataggttg tggtcgcatc
      181 tcgcaacggg ccgcacaagg ctctctttat aggggagctg gccgccaaag gcggtctgag
      241 aggttaaagg tcgaccccta cgcacagggc cgctggccgt gtatttattt tgcaacctgt
      301 attctctttc atcaaataat tagaccgcaa aaaaataagc cgtcacagga agcaaaaatc
      361 acttcctgtg acggcttatt ttttacggtt gtggataatt tcctggcaag ctggctagaa
      421 aatcgcacat atcctgacgg gctcgccacg tgcgagcccc tacagtatac gaccacaaac
      481 tccgtactac aaattatctt tgaaagaact ggcgcgctgc ggcttcgtct tttttcatct
      541 gggcgacgag gtcgtcggca gtggcgaagg ttttttcgcc gcggaggcgc ttgcggaagc
      601 gcaggctcac ttctttgccg tagaggtcag cgtcccagtc gaggacgtga gtctcgatgc
      661 ggcggtactg gttctcgaag gtcgggttgt cgccgatgtt ggtcatggcc ggatggccct
      721 tgccgtccca ccacagttcg gtgatgtaga cgccgtctgg tgggatggcc atgttgatgg
      781 ggatgagcat gttggccgta gcgaaaccga tggtcctccc ccgccggtcg ccgggcacga
      841 tggtgccggt cagggcaaag ggacggccca ggagattctc ggccgtttcc atgtggccca
      901 tctggatgag acggcggatg acggtgctgc tgatcggtgt cgtccggccc ggccgttcga
      961 ggagggtcag ggcgacaacg gggatgccgt actggctcat gaagaatttc atggtctccg
     1021 tattgcctgc cgccttggca ccataggtaa agttcgagcc gacgacgatg gccgacggat
     1081 ggatgtaggt caggaggttc tggcagaact cttccggcga ttcctgcaag agggtctggc
     1141 tcatgggcag gaggaccagg ccgtcgatgc cgacggcttc gatatattcc gctttctggc
     1201 cgattgtggc caggcgcagc ggttcttttt ccggacacaa cacggacagc ggatgggccg
     1261 taaaggtgac gaccatggtt ttcacaccgc cagcagcggc ccgtttgatg gccgtcttca
     1321 tgaccatctg gtggccccgg tggacgccgt caaaggtccc aagggccacg acggctccgg
     1381 taaagccggt tatttcctga aaggagtgga atatttccat cgttagttac ctcacaatcc
     1441 ctctgcgaac atcttatgag gccgcaggat atggtgttcc ccgtcgtagt gggcaatgcc
     1501 catgagccgc tggtccggcc cgtacacgcg gatgacgtcg ccatcggcca ggtacggccc
     1561 ggcgaagggc acgggacggc cctggagcag gaaggcacac tgtttggcgg agcaattcgc
     1621 ctttttaagg tcctggatag ccatatcggc cggccggaca taggccatgg gatcccgttc
     1681 cagctcttcc agcgtcgccg cgtcgtcgat ggtgaacagc ccggcccggg tgcggaccag
     1741 ataggtcatg caggcgcagg tccccaggac ctgacccagg tcgcggatga gggaacgcac
     1801 gtaggtccct ttggaacagg tcacggcaaa ggtgccgcgc cggccgtcaa aggacagcag
     1861 gcgcaggtcg tggatggtca cgtggcgcac gggcagggtg aaatcgacgt cctgccgggc
     1921 cagtttatag gccttgacgc cgttgacgtt gatggcggaa tatttggacg gtgcctgttc
     1981 gatgtcgccc acaaaggacg ccagggcgcg gcgcacgtcg tcagccgaca gatccggtac
     2041 ggccgccgtt tcgacgacgg tccccgtact gtcttccgta tccgtcgcca accctaaggc
     2101 gaattcggca tggtatgatt tgacgtcgct gtcaccgtat tcgatgaggc gcgtcgcctg
     2161 accggcatag accggcagga caccggccgc caaagggtcc agggtgccgg cgtggcccat
     2221 cttcttcatg ccatagatac ggcgcaggca gccgatgacg tcgtgactgg acatgcccgt
     2281 cggtttcagt acattgatga ttccgtgtat cataggcgct tttcgatttc ctttacgatg
     2341 agtttcttcg cttcatccaa aggcttatcc aaggtgcagc cggctgcgcg gacgtggccg
     2401 ccgccaccga agacagcggc tacggcgttg acgtccatgc ccttcgagcg caggctgacc
     2461 cgcgtttcct tatcgctgac gtatttgacc gtaaaagcca cttccgaccc tttgacgttg
     2521 cgggcgtaat ccatgtacat ccccgtgtgt tcgccagtga tcttgaggac ttccggcgtg
     2581 taggacaggc aggaaatgcg gccgtcatca aagagctcca ggtgttccag caccttggac
     2641 agggccttca gtttttccag ggaccgggcg tcgaggtgtt ccgaaatgaa atcgggccgg
     2701 gcgccgtggt cgacgaggag ggctgccatg cgcagcgtat ggcctgtcgt gttgttgaac
     2761 ttgaagaagc cgcagtccgt ggcgatagcc gtatagaggg catcggccat ggccgtgttc
     2821 cagccccgct gccagaggtc gaagagatag gtcaggattt cgcctgtcgc cgcgcagtcg
     2881 ggccggacgt attcccactg ggcgaaatgc tcgttggaaa tatggtggtc gatgttgagg
     2941 accttgccgc ggaccagctt ttcagcctgg ccgatgcggc cgaggcccgt cgcatcgagg
     3001 acgacggtca gccaggaatc atccgtttcg acctgctccg gcttgttgat gtactgcagg
     3061 ccgtcgagga agtcatagcg gtcgtcgata tcgtcgtcga tgaccatgac ggccttcttc
     3121 ccctgcagct gcagccagtg ccacaaggcc aggaggctgc cgatggcgtc gccatcggga
     3181 ctgacatggg ctgtcagcag gatcttatcg taatggcgca gtaaggtata gatttcctga
     3241 gccgaacaat tcatgggtat cagtccttct tttcaatgtc gcgaagaatc ttatcgatct
     3301 tcatcccgta atcgagggat gtatcttctt taaacacaat ggtcggcgaa tagcggatac
     3361 ccaggcgctt gccgacttcc gtgcggatat agccggtcgc gctcttcagg gctttcagca
     3421 tttcctgctt ggcttcgtcg ctgccgaaaa ggctgacgta gactgtcgca tcgcgcaggt
     3481 cgccggtgat gcgggcatcg gtgacggtga cgaaaccgat gcgcggatct ttcagttcat
     3541 tcaggagcat cttcgatact tcctgtttga taaattcctg tatcttgcgt actcgtaatt
     3601 ctcccatagt acgtacctcc ctttattcgg gttcgacttc gaccatttcg taggcttcga
     3661 tttcgtcgcc ttccttgatg tcgcggtatt tttccagggt aatgccgcat t
//
LOCUS       DAWABZ010000164         3675 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_5954_length_3675_cov_2.683978, whole genome shotgun sequence.
ACCESSION   DAWABZ010000164 DAWABZ010000000
VERSION     DAWABZ010000164.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5954_length_3675_cov_2.683978"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    297..306
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          875 a          884 c          863 g         1043 t
ORIGIN      
        1 attttgttgc cgtccttatc ttgacctata tagacccgaa gcttccaatg accatttcct
       61 cgattgatta cagtagccaa caattacacc tcctaaaaaa gaggctcact tagagagcct
      121 ctttgatatg ctacgatact ataaattgta tttttattac catttcattc ataagtcatt
      181 gccccttttt attttccata aaatcgctta accatgcggt ttataggccc tttaaagctt
      241 gtattccata aatgtagagc ttgtattcag ggtcaaactg cacccctcct gcacccnnnn
      301 nnnnnnaaaa atcactaaaa atcagcgaaa atgtacagta aaacaattac tcagaaaatc
      361 aataaaaaag gcggaaaaag ctagatttta tctgcttttc cgcctattgt taaccattat
      421 aaaaggccgt gaggccttat tttaccagta tttcaagaaa attcttcgaa aacctttaca
      481 aaccgcatga atactggtaa aatgaataag taaaagttca tgcgggtgta attcagtggt
      541 agaatacgag cttcccaagc ttggtacgag ggttcgatcc ccttcatccg ctccagaata
      601 aaaaaagggc tgtcgcatgt gcgacagcct ttttttgtag gggcgcccat gtggggcgcc
      661 cgctcacaca aatccggttc gcaagtatta tcactttaaa tttaaagcaa cctctccggc
      721 ctttcaggcc acctccccta taaggggagg caagagctag tagccagcta ctagccacga
      781 gccactaacc acgagccaca ttccctactt agccggcata gccgcgtttt acgccttcac
      841 gggcatcttt ttcccaatct tcgaaatgtt ctttagcagc cttgccaatc tgttcgtcgt
      901 tgttgtaatc gccgagttcg gtctggactt catcttcctt ggcttcggaa gcgttgacgg
      961 ctgctgcgaa atcacgaccg tattggcggc atgtttcctg ggttgcctgg tccgggttcc
     1021 actggcagcg caggccgtcg ttgacgacgt cgaagccggc accctggagg agagctgtaa
     1081 tctgtttgac agcttcaccg ctccagccat agctgccgaa agcagcggct ttcttattct
     1141 tgaatttcag gccgcggatc atttccatga tgccgccgat ggagaagagg aagccatagt
     1201 tgatggtcgg cgagccgacg aggatggctt tcgatttgaa gacttccgta acgatgtcgt
     1261 tcttgtcatc gacagcggag ttaaaaagtt tcactgtcag cgtcggatcc gtttcgcgca
     1321 ggccttcggc gatgcattca gccatgcggc gcgtggaatt ccacatcgta tcataaatga
     1381 tggtgatctg gttttcctgg taagctttgg cccaatcctg atatttggcg acgattttca
     1441 ggggatcttt gcgccagatg acaccgtggc tcgggcaaat catgttgacc gggacaccca
     1501 tcttgaggat ttcatcgatt ttccgcgtaa ccatggggct gtagaggttg aggatattgg
     1561 catagtattt catggcttcc tgatagagtt ctgcctggtc tacgcagtca ttgtacattc
     1621 tttcgctggc atagtgctga ccgaaaccgt cgttgctgaa gaggatgttt tcgccggtca
     1681 tataagtgaa catcgtatcc ggccaatgga gcatagcggc ttcgatgaag acgagctggc
     1741 tgtcgcccag gtccagggta tcgccggtct tgacgttgac gaagttccaa tcctggtgat
     1801 agaggccgcg caggatggct tcccctttct tggtgcagta aatcggcgta tccgggattt
     1861 cccgcatcag tgccggcaaa gcgccgctgt ggtcgttttc attgtggttc atgccgatat
     1921 agtcgatgtc tttgaggtct atttccttct tcaggttagc gacgaattcg tcgtcaaagg
     1981 gtttccagac cgtatccatg aggaccgtct ttttatcgcg gatcaagtag gagttgtacg
     2041 aagagccctt ttctgtcgaa tattcatggc cgtggaattc gttcaattcc cagtcgattt
     2101 tacctaccca agtcactttt tctgtaattt ttttacccat gatgatcgtc tccttttcag
     2161 ttatctctat atgataaaat tattctaacg taagcaacaa tgccatttta aagggtccat
     2221 cggcagtgat ggcatggcgg ccgttcttgg caaaagcaaa gttttcgccg gccttgattt
     2281 catgatcttc gccttcatag cggatgacgg ctttgccttc cagggcaaaa atcaaggctt
     2341 caccgggagc agcgtgttcc gaaagacctg tgccggcgcc gaagctcatg acgacgaatt
     2401 tgactttagg cgactggata atgtcgcggt tgatgatctt accatcctga taaggcacga
     2461 ggcctgccaa agagaaaatc ttgcctgcat cgatgttcat aaggaattcc tcctctactg
     2521 taagttccgt atagaccgcc ccatcgcggc tcttcatgcc aacgggtacg gacggcggtg
     2581 cgatgatggc ctgaccagcc accagattat gttcttcccc cggccagtga acggtcagcg
     2641 taccgtcagc gacatagagg agtttcgcct gcctgtagat ttccggactg atgtccgtac
     2701 cggcagccag ggaaaagtaa aagacttgat actgggcttc attgaccagt tctttagaaa
     2761 tcgtacagcc ggccactggg gcattatcac ggctgataga aaagatatgg cctttcgttt
     2821 ctttcattcc actcacctcg ttttttcttt cccttcgatg tgttaagtat acgttttttt
     2881 catcattatg tctgttgtaa ttacaacaga taatagaatt tctcaattat aaaaaaaata
     2941 ggcgtcgcat taagcgatgc ctatttttag cagccagcag tcagcggtta gcagatagct
     3001 ttaaatcaac ctctcagacc gccttcggcg gccagctccc ctattatggg agccttgagg
     3061 actgcctcta cgagctgcta gccactaact gctagccact agccactaaa aaaagacctg
     3121 ccgtgtatgc ggtaggtctt tttttcatcc tcactggatt tcaatggtgt tggtcggttg
     3181 tgcctgttgg gcatctttcg ggagagttac ggtcaggacg ccatctttga aagcagcctg
     3241 gataccttct ttctgaatac ctgtgacagg gaactgacgg cagaaggacg aactcgaacg
     3301 ttctttgcag atgtagttgt gctgttcgtc ttgcgaatcc ttctgttcgt tgtggctggc
     3361 agccaaagtc aggacgttgt tgtcgtagct cagatggatg tcttctttct ggacgcccgg
     3421 cagatcagcg gtcataacgt attcgctgcc tttgtcttca atgtcgactt tcggcatcgt
     3481 gctggagaat tcactcggcc acatgaagcg attgaagaag ttatcaaaaa catcgggaac
     3541 ggaaaattca ttatcaacaa ctgggaataa gctattcata agtcatgcct tctttttacg
     3601 taagtttgaa caccatatat caaacattga atacaaggga gtttggcggg tgccccacgt
     3661 gtgttatgtg caata
//
LOCUS       DAWABZ010000165         3672 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_5958_length_3672_cov_3.035112, whole genome shotgun sequence.
ACCESSION   DAWABZ010000165 DAWABZ010000000
VERSION     DAWABZ010000165.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5958_length_3672_cov_3.035112"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          787 a         1071 c         1037 g          777 t
ORIGIN      
        1 gtcttcatag acagcgcctt ttccacaccc gaaaggagct attatgaatc gaactgactg
       61 ggatgcttat tttgcctctc ttattggcga tacggggacc ggcgtgcctg gtctggccgt
      121 cctggcctat gccgatgggc gtgaggtcta tcgttttgcc gccggcgtcc gccgcgtgga
      181 cggggagcgg tcctggccga tgacggtgga cagccgtttc cgcatggcat ctgtctccaa
      241 gatgttcacg gtctttaccc tcatggagct cgccgaagcg gggaagctgt ccctggacga
      301 cgatgtcagc cagtacctgg gctttcgcct gcgccatccg tcttaccctg acgtgcccat
      361 tacgcttcgt atgctggctt cgcatacgtc gggcctgcgc gacggcaggg tttacagcat
      421 tccgccgtcg gtccgtgtca aggccttctt tacgccaggt agtccctatt gggaagacgg
      481 cgcccatttt gccccggccg ggcaggcacc gggtatttat tttacttatt gtaacctcaa
      541 ttacggcctt ttggggacgg tcatcgaagc cgtgacgggc cagcgcttcg ataggtatca
      601 gaaagcgcat atcctggccg ccctggactg ccgggccgac tatgtgccgg gcaatctgga
      661 agcggctgaa ttcgccaaat tggggacggt ctatctcaaa cgggatagtc agggccgctg
      721 ggatgaacag ggcccctggc aggggactat cgacgatttt ggcggcgtcc agccggagcg
      781 cgaagccctg gccttgcaga atccctatgc ccaggacgtc tgccagactt acgacctggc
      841 cgcgtaccgg cctgggacca atgcgacgat cttttcaccg caaggcggcc tgcgcctgtc
      901 tctggaggaa ctgggccacg tcctggccat gatgattcag ggcggccgct atcaggaccg
      961 gccggtcctg gcgccgtcgt cgctgcaggc catgttcagc cctcagtggg tctatgacgg
     1021 ccacaatggc gatacgtgtg gccagaccct gctcacctat ggcctgggcg aattcttcgt
     1081 cgacggcacg gggccggccc gctgttgccg cacgaaggcc gtcgatttat ggggccacac
     1141 gggccaggcc tttggcctct tgtcgggcct cttcgtccgg ctcgggacgg gcagcggctt
     1201 tgcctacgtc atgaatggcg aggccctggc cgaagatgac gacctgcgca gtgccggccg
     1261 cttcagctgc aactatattt gggaagaaaa tgtcatggac ggcctgtgcc gattgattga
     1321 cggcctataa gggctgtgct atgattatga aatatagtaa cattccaaat gggaaggcga
     1381 gataatgatg aatgcatatg aaagtaccat gaattttaca cgccgtgccg tctgggccca
     1441 gatcgacctc gacgcagcgg ctcataatat gcgccagata cgcaagcacg tcgggccgga
     1501 tgttaagatc gccgccgtcg tcaaggccaa tgcctacggc cacggcagtg tcgaactggc
     1561 caagaccttt gccgaaaacg gcgccgactg ctttgccgtg tccagcctgg acgaagccgt
     1621 cgagctccgc cgctatgcgc atatcgacaa cgaaatcttc atcctcgggc ataccgacgc
     1681 ccggcggaca gaagaactcc tgacgtatga catcgaaccg gctgtcttca acttgaagaa
     1741 cgccgaattc ttctcccagg aagcccggcg cctgggcaag accctgcgcg tccatatcgc
     1801 cgtcgatacg ggcatgagcc gcgtcggatt ccaggtcaat gaattcagcg tctcggaaat
     1861 caagaccatc gcaaccctgc cgaatatcga aatccgcggc atgttcaccc attttgccgt
     1921 ttccgacatg aaagacaaga cgtttacgcg cgaacagtac ggccatttcc gctggatgtg
     1981 caagcgcatg gaagaagaag gcatccacat cgccctgcgc cattgctgca acagtgcggc
     2041 cgtcctggaa ctgcccgaat actactgcga catggtccgg ccgggcatca tcatgtacgg
     2101 ctgtgaaccg tcgccggaaa tcgacatcaa gccttatgac ctgcggcccg tcatgtccct
     2161 gcgctgctgc atcgcccatg tcaagctcat cgacgccggc gcgaccgtca gctatggccg
     2221 ccattataaa actccgtcgc gccggaaaat cgcgaccctg cccgtcggct atgccgatgg
     2281 ttattcgcgc atcctgtcgg ggaaagtcga cgtcctttac catggccacc gcgtccccca
     2341 ggtcggtgcc atctgcatgg accagtgcat gatcgacgtc agcggcgaag ccaacgtcca
     2401 cgccggtgac gaagtcgtcc tcttcggccg ccagggcgac agcttcatcc ccatcgaaga
     2461 aatcgccgca gtctgtggca ccatcaacta cgaaatcatg tgcaacatct cgcgccgtgt
     2521 cccccgcgtc tacatgaaga acggcaaagt cgtcggccgc gaagaatacc tcttcgataa
     2581 accgtaggga taggcagttc gcaggccgca gttcgtaggg gacgcccaaa gggcggcccg
     2641 ccagagtcat ctgtcaggct gaaacggatc gcggtagagg ggcgaaaaaa cgttatcatt
     2701 atctgtaggg gctcgcacgt ggcgagccca caggtatatg taggatactt gtaaaacaga
     2761 ttttgcgtga acgggcgccc cacgtgggcg cccctacaag aaaaaagggc ttgtcgctgt
     2821 gcgacaagcc cttttttcct cttccatgat tttgttgcaa gtggtatact gtatataaga
     2881 tatacgcata tataaatgca aaataatcat aaaaaggcgg tgtgttatta tgaattggaa
     2941 tgataaaacg gcggccaacg ttaaggccat caaaccctcg ggtatccgca agttcttcga
     3001 tattgcagcc caggtcaaag gggtcttgtc cctgagtatc ggagaaccgg actttgccgc
     3061 accggacaag gtactggcag ctatgaaagc cagccttgat gccaaggaaa cgagttatac
     3121 cgggaattca ggcatgatgg agctccgtga agccattagc catcattttg aacaacgcta
     3181 tggtgtccat tatgacccta aagatgaaat cctggtcact gtcggcgttt ccgaaggcct
     3241 ggccatggcc ctcctggcca ttaccgaacc gggcgatgaa gtcatcatcc ccgacccggc
     3301 ttacgttgcc tatccggcgt ctgtcgaact ggctggcggg acgccggtct tcgtgccgac
     3361 ctatccggaa cacgatttca aactgactgt cgaagctttg gaaaaggtca tcacgccgaa
     3421 gacgaaagcc atcgtcatcg gctatccgaa caacccgacg gggaccgtca tgtcggcaga
     3481 agaattgatg cctattgcca aatttgccga agaacacgat ctcatcgtct tgtccgatga
     3541 aatctactgt gaactcatgt acggcgatgc caagttcacg tccttcgcca aattgccggg
     3601 catgcgtgaa cggacggtca tcttcaacgg cttttccaag gcctatgcca tgacgggtat
     3661 gcgcctgggc ta
//
LOCUS       DAWABZ010000166         3625 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_6026_length_3625_cov_3.398880, whole genome shotgun sequence.
ACCESSION   DAWABZ010000166 DAWABZ010000000
VERSION     DAWABZ010000166.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6026_length_3625_cov_3.398880"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          849 a         1067 c          991 g          718 t
ORIGIN      
        1 gtcatgaccc gcgaagacca cgccaatggc acggaccgtt tggctgaagc cgtcggccat
       61 tttcccgatg ctgacgtcat cgtcaacgtc cagggcgacg aaccgctcat cgcaccggac
      121 gtcatcgacg acttgtgccg ggcctttgaa gcagaaccgg acctgaccat ggcgacggtc
      181 gctgcgcctt tgcaggaaga cgaatacgat gacccgagcg ccgtcaaagt cgtcctcaac
      241 caaaaggatg aagccatgta tttctcgcgt tccctcatcc cgtatccgcg ccattccttt
      301 gaagaagggg gcatccagcc gtataagcat atcggtatct acgcctatcg ccgcgatttt
      361 ctcctgcagt acgcggccat ggaacaaacg ccggcagaaa aagtcgagtc cctggaacag
      421 ttgcgggttc tcgaaaatgg ttataagata aaagtgataa agactcatca ccaattcatc
      481 ggcatcgata cgccggatga cctggaacac atccgccgtt attttgccga atctgctcat
      541 aaaggggaat agactatgga aaaagtgaaa cgaattcagg ttggcagcca gactgtcggc
      601 ggcgatggca aacttttcgt catggccggc ccgtgtgtca tcgaggatcc cgaacggacc
      661 cgggctattg gcaagaccat gaaggctatc tgtgaaaaac tgggcctgac gtatatcttc
      721 aaagcctctt tcgataaggc gaaccgctcg tcttacacgt cctaccgtgg accgggcctg
      781 aaagaaggcc tgcgcatcct ggctgacatc aagaaggaac tccaggttcc cgtcgtcagc
      841 gatatccacg aaacctgcca ggccgaagcg gctgctgaag tcctggacgt catccagatt
      901 ccggccttcc tctgccgtca gaccgatttg ctagaagctg ctgcggctac ggggaaatgt
      961 gtcaacgtca agaagggtca gttcctggcc ccggaagaca tgaagaatgt cgtcgccaag
     1021 atcgaacacg ctggcaatga aaatatcatg ctcaccgaac ggggcatcag tctgggctat
     1081 cacaacctcg tcgtcgacat gcgttccttc ccgattatgc gcagcttcgg ctatcccgtc
     1141 gtctttgatg ccacccacag cgtccagctg ccgggcggtg ccggtacgaa atcggcaggc
     1201 cagcgccagt tcgtcggcaa cctggcacga gccgctgccg gtgccggtat cgacggcgtc
     1261 ttcatggaag tacacgataa cccggcagaa gccctcagcg acgggccgaa tatgctctac
     1321 ttatcgcagg tcgaagatgt cctccgcgat atgatggcta tcaacgaaat cgcacagaaa
     1381 agcgtaagca cggacaggta aggagacgag accatgatgg atattttaga agaagcaaaa
     1441 gacgtgctcc atcaggaagc acaaggcatt gaaaagttga tccctacgct caaccagaac
     1501 ttcgtcaatg ctgtcaacat gattatgaaa tcgaaaggcc gggtcatcgt cacgggcatg
     1561 gggaaatccg gccacattgc ccgcaaagta tcggcaacct tgtccagtac ggggacgccg
     1621 tccattttcc tccatccggc agaagccatc cacggcgatt tgggcatggt cacggcagac
     1681 gatgtcgtcc tggccttctc gaacagtggg gaaacggtgg aaatactcaa tatcctgccg
     1741 tcgctcaagc gcatcggtgc caaactcatc accgtcgtcg gcaatcacaa ttcgaccttg
     1801 gccaaacatg ccgatatcat cctcgacgca tcggtcgaaa aagaagcctg ccccctgggc
     1861 ctggcgccga cgacgagtac gacggcggct ctggccttag gcgatgccct ggctgtcgtc
     1921 ctcttgacgc agcaccattt caccaagaaa cagttcggca tcttccatcc cggcggagcc
     1981 ctgggcaaga aactcctgct cactgtcgac gatgtcatgc ataaaggcca ggacaacccg
     2041 gtcgtcagcg aagacagcat cgtccaggat gccctgttcc tcatgaccga aaagggcctg
     2101 ggtgccgtat ccgttgtcaa tgaagacggc aaactcgtcg gcctggtcac cgacggcgac
     2161 gtccgccgcg gcctggaaac agggtccaac ttcctccagt ggcctgtcga cgccatgatg
     2221 accaagaacc cgcgggtcat tgccaaccat aagctggcta cggaagccct gcacatcatg
     2281 gaaaagaacc agccccgtcc gattacggtc ctgcctgtcg tcgacaagga tgacaaagtc
     2341 gtcggcatga tccatattac cgatttgctg cggcagggag tggtctgatg agctttttga
     2401 cacatatcaa gatgctggcc ctcgacgtcg acggcgtcat gacggacggc atcatttact
     2461 acagcccgtc gggcgatgcc atgaaaggct tttctgcccg cgacggcatg ggcatttccc
     2521 tgatccgggc cgccggcatc cagacggccc tggtcacagg ccgccgctca cccatggtcg
     2581 agcagcgggc gaaggatctg catatcgact acgtcctcca ggactgctcg cgcaagcttt
     2641 tggccatgca gcagttgtgc agtgaaatcg gcctgtccct cgacgaagtg gcctacatgg
     2701 gtgacgacct gaacgacgtc gaactcattg ccaatgccgg tttcggcgca gcgcccctcg
     2761 atgcctgcga agaagcccgt cggtcggccg ctttcgtatc ggcctatccc ggcggccacg
     2821 gggccttgcg ggaattcgcc gaatatatct tgaagagcca gcatgtctgg gataaggtcc
     2881 tgcgccagta ctttggcgac gcgaccttta cgttgcgcca ataagaaagg agagtctgta
     2941 ttgtgagtaa ggaatggcag tatcatgctg cacgcggatt cggcaagatt ctctgtcatc
     3001 tgccctatcc ggttatcatt tccttgggtc gcaaactagg ccctttcgta ggaaaactga
     3061 tgaagaagca gcgcgaacgc ggtattttcc agatacagcg cggcatggag tgcagccgtg
     3121 aagaagcgga acgcatcatg accgacgtct tccgcaatct cggccagtcg ctcatggaaa
     3181 tcctctatac gcccaatctc aatccggaga atatccggga ctatgtgaca ttggaccatc
     3241 cggaacgcct ggacgaagcc atgaaggaag gcaaaggcgt catcgtcctc acagcccaca
     3301 tgggcaactg ggaatggctc ggcgccctgg ccatctacgg ctatccggcg acgacgatcg
     3361 tcaagaacca ggccaacgac gccgtaacgc gcctgctcaa cgaaaaccgt gagaacatgg
     3421 gactggaagt cttcgcccgc ggcggcaatg aaatgatcat cgctgcccgt gccttgaagc
     3481 gcaagaaact cctgggtttc ctggccgacc aggacggcgg cttccacggc gtgcctcagc
     3541 cttttttggg caagatgagc tcgacgccga aaggaccggc tatgttcgcc cagaagttcc
     3601 attcacctat cgtgccgatt ttccc
//
LOCUS       DAWABZ010000167         3602 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_6056_length_3602_cov_3.165210, whole genome shotgun sequence.
ACCESSION   DAWABZ010000167 DAWABZ010000000
VERSION     DAWABZ010000167.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6056_length_3602_cov_3.165210"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    389..398
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2553..2562
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          794 a          970 c          933 g          885 t
ORIGIN      
        1 ggatgtcgtt gaggacttct ttcatggctt tcttcgattc ctgggttacg acacgcgggc
       61 cagccgtgta atcgccgtat tcagcggtat cactgatgga tttacgcatc ttagccatgc
      121 cgccttcgta catgaggtcg acgatcaact tcatttcatg gaaggtttca aagtaagcga
      181 tttccggctg ataaccagct tcgcaaaggg tttcaaaacc gttggtgatg agctggcaga
      241 tgccgccgca gaggactgcc tgttcaccaa agaggtctgt ttctgtttct tcctggaaag
      301 tcgtctggat gacgccggca cgcgtgccgc cgatgccgcg ggcataagcc aaagcgaggt
      361 cgaagcattt gccggaagca tcctgttcnn nnnnnnnncg ccgccttcta cgaatgtacg
      421 acggacgaga tggcccgggc ctttcggagc gaccatgaat acgtcgacat cagccggcgg
      481 gacgatctgt ttgaagtgaa cgttgaagcc gtgtgcaaag gcgaggacac tgccggattt
      541 caagttcgga gcgatttccg ttgcatatac gtcagcctgt ttttcgtccg gaatgaggac
      601 catcgtgacg tcagcatctt tgacagcttc agcaacggtc ttgacttcga ggccggctgc
      661 tttagctttt tcaaccgatt tgctgccttc atacaagccg acgacgacgt cgacgccgct
      721 gtccttgagg ttcaatgcat gagcatgacc ctggctgcca taaccgatga cggcaacttt
      781 tttgcctttc ataacgtccc aatttacgtc ctgatcataa aatacttttg ccataataac
      841 atctctcctt gtgagtataa aataatttat tctcgttggg atttagagat tcgtcgaaag
      901 aggccaacaa aaaaactccg cccctcactg agggacggag tactattccg cgataccacc
      961 ctgattcccg gcgcattact gcagccaggc tctctacaac gtaccaacat acgcgtttcc
     1021 tataacgtgg aaaaatcgtc agtgcttaat cttgcttcag cacttctctt cggggatgat
     1081 tttcaatcgc cattcattac cggctcgcac cagccgccgg ttctctgtga atgtccggac
     1141 gaactactct ttcccgtcct cagatttcac tatatgcttt tgcgagcctt tatcgttgtg
     1201 gcccgtttga aaagcttgaa cacattctac aaaatgccgt gctgtttgtc aatgcttttt
     1261 tggaattttt ccgaaaaaag tttggttaaa cgtttacttg cctaagattc ttaagccttg
     1321 cgattgccca gataccattt cacaaactgc tgagcaatgc ggccattgcg gccggaatgt
     1381 tccatttccc agcggacgcc ttcgacacga agctcttccg ggtccagggt aatgccggct
     1441 tttttcagct catgggcgat gatctgcaag tattcatcct gcgtcggcgc cgagtaatgg
     1501 aggatgaggc cgaagcggtc cgacagggaa atcgactcgt tcgtgctgtc atcgcggtag
     1561 acttcgtcca gttcgtcgtt gcggtcgtgc cacgtttcct tcaagaggtg gcgacggttc
     1621 gatgttgcat aaataagcac gtttttcggc tgtggcgtga cgccgccgtc gatggccgac
     1681 ttgaggtatt tatactcttt ttcattttca tcgaaggaca aatcatccaa gaagaggatg
     1741 aacttcttgc tgccgatgtc gccgagtttt tccatgagct gctgcagatt gtggagctgg
     1801 ccgcgctgga cctggatgag gcgcaggccc tgttcatggt agcggcagac cagggctttg
     1861 acagccgtcg acttgcccgt cccgcgggaa cccgtcaaga ggacgttatt ggccgcccgg
     1921 tcagccagaa aggccgtcgt attggccagg agcttttctt tctgagccgc atagccgatg
     1981 aggtcatccc aggcaagatg ggggaaattc tcgatgccta cgaggtcccc gtcttcaccg
     2041 atgcggaagg ccatgaactg tgccagtttg ccccggccgt atactgcata atgatgcagt
     2101 aagactttcg tcgcttcttc tggcgtcgac gctgccatga gggccgtttc cagggtctgg
     2161 acatagccgt aaactttggg gacagccggt tcgtagttgt cgaggaaatc gacgccgagg
     2221 aagtcggaag cggccttttg cagatacggc cagagcagag tcatatccaa gagcagggcc
     2281 ttggtcatgc ccgtgccggc cttgcccaac cgttcaatgg cttcggcagc gaccgtatcg
     2341 ccttccccca agagatagag gaaatactgg cgcaggatat tgccgctgag gcccagctgt
     2401 tccgcccgtt ccaagaggac cgctgccgct tcatgcttgc caaagggatt atccccctgt
     2461 tcctggacgg ccagcagggc cttgatgacg ggatcctgta aaaaattgcg gcatacgacg
     2521 aggccgcgca gacttttatt ttccatgtag aannnnnnnn nngtagaaac ctccctatca
     2581 gcttatatac atttgagcca ttataccgcc gccaggccac ggcagtcaag gaagttccgt
     2641 catatttata cagtaaaagg ttttacctga ctaatttatc taacgcagag catcggccat
     2701 ctggcacatg cggacgatgg gtttgacgtc atggacgcgg acaatcgcgc agccggccag
     2761 gacgccagcg acacaggtcg tcccggtccc ttccatccgt tccgtaaccg gcaggtcgag
     2821 gatcttgccg ataaagccct tgcggctcgt ccccaggagc atgggatacg gcaggcctgt
     2881 cagatcccgc aagtgcttca tgacgtagac attctggtca aaggtcttgc caaagccgat
     2941 gcccgggtcg gtgatgatct ggtcctggct catgccggcc tggtctgcaa tgatggccga
     3001 gcggatgaag aagcgcttca tatcctcgat gatgtcgccg tattctgtac cgtcctggtt
     3061 atgcatgacg acgacaggaa cgccgtattt tgcggctaca gcggccatct gtcccggttc
     3121 cggcgcatac tggaggcccc agatatcatt catgatatgg gcacccgtac gcatggcata
     3181 gtctgccgtt tcagccttat acgtatcgac agaaatgggg accgggcagg ctttgacaag
     3241 gcgcggcaaa atcgtttcca gccgggaaat ctcttcggct gccgaaatcg gcgtaaaacc
     3301 cggccgtgac gattcggcgc cgatgtcgat gatatccgcc ccttcggcta ccatttcttc
     3361 catgtgcttc aaggctacat cggcattatt ccatttcccg ccatcagaaa aagagtcagg
     3421 cgtataattg aggacgccca tgaccagcgt ccgttccccg acagtcaggg attttccatc
     3481 tttccaatga taatgacgaa cttccacaat aattcctccc aacaaaaaaa gagataacga
     3541 aggcgcccaa aaggacgcac agtcgttatc tctaaaaaat caagtccgta acctgtacgt
     3601 ta
//
LOCUS       DAWABZ010000168         3522 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_6206_length_3522_cov_3.660225, whole genome shotgun sequence.
ACCESSION   DAWABZ010000168 DAWABZ010000000
VERSION     DAWABZ010000168.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6206_length_3522_cov_3.660225"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          757 a          982 c         1073 g          710 t
ORIGIN      
        1 gatgacgcga agaggcccag ggaaatggca gcaaaagaga aggcaccggc caaacggccc
       61 agcgactgga tggacgcccg gtcagggatg cccagcaggc gggccaggac acagaccagg
      121 ccaaaggata aaatggacag gacccagtac acgccgactt tgacgaccag cagcttgccc
      181 ttcgacgtcc ctttcagctc aggattgcgg aaatcgcact ggacagcagc tccgatggcc
      241 aggaaaaagc cctgctgcat ggccgccatg gccaggccgg tgacgaagaa catcatgtaa
      301 ctctgggtcg ggttattgag gacgcgcagt gtcgtctcta ccggtgagat gcgcttcttc
      361 agcatctgtt catcgccgct ggtccgcaat gccgcctgcc gggcggcaaa gcgcgtattg
      421 aaggtattga cgatgtcgtt gccagccgtc gtggcgatat tggtcgtaat gatattggcc
      481 ccgttgagca tgaacaggac tttagcgctg cggccgttga ggatatcgtg agaaaaatcg
      541 gccggaatga tgacggcggc gtagacctct ttccggcgca gcatgtcccg tgcctcccct
      601 tcatcggcga cgacgcggac cagccccagc cgttcgtcat catcgacctg gcgggtcaaa
      661 aggcggctgt aggccgtatg ttcctcatcg cagacgaccg tcggtatctc cttgacgacg
      721 ccgggataat agaggatgcc gaagagaatc aggtaggccg tagcggcgcc aaagagaaag
      781 atagcccgcc gggcatcgtg gagaaaggca ttggccagtt cccgtcgtat caatgcaata
      841 aatcccatta tgaccgcgct cctttccgct tacgcgcaat agcccctgcc gctatttcca
      901 ggaacgtctt catgccgtag cggcggcgca gggcaaagat agccgttccc aggacaaagg
      961 ccccggcccc catgagcagc agctgcaggg actgggaaac cagcgccggc gcataaccgg
     1021 ccaataagat atcgcggacg ctgtcggcag catggcgcat gggaaaaatc tgggcaatgg
     1081 cggcagcgac gtcgctcatg tagaaatcag gccagctcag gccgctgtac aagaggcccg
     1141 gcatgatata gagcaagggc atttcgatgg acatgactgc atccggactg agcacggcaa
     1201 agaggaccat aatgctggcg acggccaggc agaaagccgt tatcatgacg gccagggcgg
     1261 ccagggaacc ctgtacgggg acggcgaagc tgtagtataa gaatcccagg gagaggaaaa
     1321 aagaaggaac cgccatgacc agtacacaga gcaccttggc gcccaggatc agccacgacg
     1381 ggatggactt ctcgtaacca tgataccgga actccctggc gatgaggggg cacaagacga
     1441 gcataatggc aatctgcagg ccattcatgg tcagaccggc cagcatgaac ggcgtatagc
     1501 cggccgtcgg attgttgacg atgcggatgc ccaggcggac ggggtaagcc atattcatgg
     1561 ccgctgccgg cagctggttg cccgcttcca ggagtttggc cgcaatgccg acgaacaagg
     1621 tcttgttgat ttcactggct gccgtcaggg cggcgttgcc gaacatgtta ttggccgagt
     1681 tgaccgtcag catgagcggc acgggattgc ccagcttgac ctgtttggac aaatcctccg
     1741 ggatgtacag gccgaccagg gccttgccgc tgttcaaatc atcgaccata tcttccgacc
     1801 gcgtcacctg gtcgacgagc tggaactttt cagcatcgct gtaagcctgg acgatggtcc
     1861 ggctgacgac ggactgctct tcgtcgcaga tgaccagggg gatccgttcg accgagttct
     1921 gatagtaaat gataccaaag gccagggtaa aggccagggg cagcaggaag aggatcgccg
     1981 cgacgggaaa gcgcccctgc cagagccagt gccattcctg cttcaagacg tgccatactt
     2041 tcatgtcagt cacctgccat atactggaaa cgcatgcccg gccgcagggc atcgctgttg
     2101 acctggacct tgacgttgaa actgatgata tccgtatcgt tgccgcgttc gctcgtcgcc
     2161 cgctgggtgg cgaattccga tttctggctg atatcggtga tggtcccctg gacgcgggtg
     2221 acgccgtcac gggcctgcat ggtcaggacc tggccgagat ggaagtcttt gagcttcgtt
     2281 tccggaatct tgaagtcgac ccagttgtcg ttcgggtcct gcagggcgac cagcggcgtc
     2341 cccagggaaa tcatggaacc ttcttcgatg tatttagccg taatgatgcc gtcgaaaggg
     2401 gccttgattt ctgtttcatc cagggatact tcgatctgct gcagccgggc ctgagccgct
     2461 tccagtttct tctgggccgc atcggtagcc gctaagttgg catcggtctg caagaggccg
     2521 gcctgggccg acgatacgct ggactgggcc tggctgtacg ccgactgggc aacttcgtac
     2581 ttggtcttat aggcatcgaa gacggatttg gaaatagctc cttgggcata gagttcctgg
     2641 taacggttga aatccttttc tgccaggtcc agattggact gggccgaatc gaggccagcc
     2701 gaagccgtat cgacttggga cttggacgta ctgtcctgga ggtccgtgac gacgccggac
     2761 tggcgcacct gggcctggag ggccttgata ttggcttcgg cctgggcctt ctgggccagg
     2821 aggtcgcgct ggtcgatgta ggcgatgatg tcgccctgct tgaccgtatc cccttccttg
     2881 acgcggagct ggacgacacg gccggtgact ttggagttga tgtcgatttc cttggcatcg
     2941 gcccggcccc acaattcatc tgacgctgct tccttctgca aactgcagcc gctggccagg
     3001 cagacaacgg ccagggccag gagcaagccg gcagcccatt tctttttttc tatgaacatg
     3061 atgagttccc ccttttgttt gttgaacatg ttcaaatctt gagcaaaaaa ggctgcccca
     3121 tgaagcccca aaatagcctc atgcggcaat cctttacaga aagccgtcct atcgccggtg
     3181 gatcatctca tcggcaatgg cttcgatttc cggcaggcgc tgttcgagct gggccaggac
     3241 gtcatggctc tcagcagccg aaatgcccag cagctgcaaa gccgtatcca ggaccagggc
     3301 ggcgtacttt tggctgtcca tgggctggct gaagtcgaac tggacgatgt agccgcgcat
     3361 ggcccctttg atgagcaggg agcgcaggaa aatatcctcc ttcgataatc ccagggaatc
     3421 gccgaagatg cggcccgtca tatcggccag atggtccgtg atcttttcaa acgtcaaggg
     3481 cgtcgtatag ccttcataat aggtttcgcg gacgagctgg tt
//
LOCUS       DAWABZ010000169         3455 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_6339_length_3455_cov_3.414706, whole genome shotgun sequence.
ACCESSION   DAWABZ010000169 DAWABZ010000000
VERSION     DAWABZ010000169.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6339_length_3455_cov_3.414706"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          734 a         1133 c          950 g          638 t
ORIGIN      
        1 ggtcaaaggg gaagtcgaat tcaaccatgt ctcctttgcc tatgacaaag accacccggc
       61 ccttaccgat ttcaatttga aagtcgcccc tggcgaagtc gtcgccctcg tcggccccag
      121 cggtgccggc aaatcgacgg tcgccaactt gctgccccgt ttctacgatg tcacgggcgg
      181 cagtctgacc atcgatggca ttgacgtccg cgacgtcacc ttctcgtccc tgcgccagca
      241 gatcggcctg gtcccgcagg aaacgatgct tttcaacgct acggtccggg agaacatcct
      301 ctacggccac ctcgatgcga cggacgaaga agtcgtcgcc gctgccaaag ccgccaatgc
      361 ccatgacttc atccgggaac tgcccggcgg ctacgaagcc ctcgtcggcg accgcggcag
      421 ctccctgtcc ggcggccagc gccagcgcat cgccattgcc cgggccatcc tcaagaaccc
      481 gcgcatcctc atcctcgatg aagcgacgtc ggctctcgat acggaaagcg aaaagatcgt
      541 ccaggctgcc ctggaccgcc tcatggaagg ccgcacggcc gtcgtcatcg cccaccgcct
      601 gagcacggtc cgcaatgccg acaacatcgt cgtcatcgac cacggccgca tcgtcgaaga
      661 ggggacccat gatgaactac tggcaaaaga cggcctctat gcccatctct atgccgtaca
      721 gttcaacgat actacggaag aaacagcgtc agaaggttga ttgtgatgaa gaatctaaaa
      781 tcgcacccca tgtactggct gtataatatc ctgctcctca tctattgggc tacgctcatt
      841 cccatgctca tttaccggct catcagggaa gaaggatttt atcagcgcat caaacagagc
      901 atcggcttct tgccggaaga attaaaagat aaaatatcca atcgccatgc catctgggtc
      961 catgccgcgt cggtcgggga aatcgtcgcg gccagcccga ttgtccggga aatgcgcaag
     1021 acccatcccg acgaagtcat catcgtctct gtcgtcacgg cgacgggctt ccacatggcc
     1081 catcagatca tcaagggcgc cgacgggatc ctctatttcc cgctggacct gccgtatctg
     1141 accaaccgca tcctgaccat cgtcaatccc caggccatcg tcctggtaga aacggaaatc
     1201 tggccgaatt tcctgcgcat cgccgcagat aagcatattc cggtcatgat gatgaacggc
     1261 cgcatcagcc agcgcagtgc tgtccgttac cgcatgatta ccttctttac gcgccgcgtc
     1321 ttgtcgacga tacgcatgtt ctgcatgcag agccgcatcg acgcccagta tatcatcgac
     1381 atcggcgccg atcccaacaa ggtcatcgtc acgggcaaca ccaagtacga ccagacctat
     1441 ggcatcgtca cggaagccga aaagaagcgc ttcctccacg aactgggctt tgccgaaggg
     1501 acgtatccca tcatgatcgc cggcagtacc cacaaagggg agaacggctc ggtctacaag
     1561 gccttcgtca agatacggga ccatttcccg gaagccaaat tgatcatcgc cccgcgctat
     1621 atctatcagg ccgatctcat catcgacgaa ggcatcaagc acggcgtcac tatggtcaag
     1681 cgtagcgata tgaaggccgg gaaaccgatc cggtccgaat acgacggcgt catcctcgat
     1741 accatcggcg aactggggcg cgtctacagc ctgggcgacc tcatcttcgt cggcggcagc
     1801 ctggcccata tcggcggcca taatatcctg gaaccggcag ctcatggcaa gcccatcgtc
     1861 gtcggtccca atatgttcaa cttcgtcgaa atcttcgatc tcctcagcag ccgcggcgcc
     1921 tgcgtcatgg tcaagaatga aaaggaattc atcgacacct gcctggatat cctcatccat
     1981 cccgaccggg ccgaagccat gaagcgcagc tgcatcgaag tcgtccagga aaaccagggg
     2041 gctacgcgca agaacctgga acagctgcag aagatgctgg acgacgtcca cggcgggagg
     2101 tgagcccatg gctttgcgaa actgggctgc cgcctatttc cattatttga ccggaagcgg
     2161 cgcccatcac tgctgggact ggccggccct ggccctgctg cgcggtgccg cctggctcta
     2221 ccgcaagggc gtcctggtca agtatcacgc catcgccgac catccggaac ggcaggaaca
     2281 cgcgccggcc gtcgtcatca gcctgggcaa catcactgtc ggcgggacgg gcaagacgcc
     2341 gatggcgtgc atgctggccc ggcgcctgca atcccagggc tggcgcgtgg ccctgctcaa
     2401 ccggggctac cgctctcagc gcgaagatgg cggcgctgcc gtcatgtccg atggacagcg
     2461 cgtcctgcta tcggccgaag aaggcggcga tgaagcctgc ctcatggccc gcagcctgcc
     2521 tggcatcccc gtcctggtcg gccgcaagcg ggccctcggc ggccgcctgg ccgtcgaacg
     2581 cttcggtaca caggtcctca tcctcgacga cggctttcag cactggcagc tgtaccgtga
     2641 cctggacatc gtcctcgtcg acgggaccaa tcccttcggc aacggccatg tcctgccgcg
     2701 gggcatcctg cgggagccca tggagcagct cggccgggcc gatgccttca tcatcactaa
     2761 gggcgaccag attacgcagg accgggccga agccatcgcc gccaagctgc gccagtacaa
     2821 cccggccgcg ccggtggcca tggccatcca caagccgtct tcgtgcctgg cctttgccgc
     2881 ctggcatgac ggaaagggcc atggctccgg ggccttacag cctgacggcc agtccgtcct
     2941 ggccatatcg gccctaggaa acccggcttc ctttgaacgg accctggccg atgcgggctt
     3001 caccgtcgcc ggctctctcc gctatgaaga ccatcaccat tacagccgcg acgatatccg
     3061 ccacatggcc gccagggccg ccgaaacggg ctgccttttg gtgacgacgg aaaaggacgc
     3121 cgtcaaactg ccggcgccgc tgatcaagga atacgacctg ccgctgtacg tcttgagcat
     3181 tactattgaa ttcacagcag ggcaggacca gatagatacg attttgcaag cagtattgga
     3241 ggataaacta tgaactttgt atgcatcatc ccatcgcgct atgcatcgac gcgcctgccg
     3301 ggcaagccgc tgtgcgatat tgccggcaaa ccgatggtac agcgtgtcta tgaacaggct
     3361 gccaaggcct cgaaactgac ggctgtcgtc gtcgccgtag ataacccgaa agtctatgat
     3421 acggtcgtca ccttcggcgg ccatgccgtc atgac
//
LOCUS       DAWABZ010000170         3342 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_6569_length_3342_cov_2.837542, whole genome shotgun sequence.
ACCESSION   DAWABZ010000170 DAWABZ010000000
VERSION     DAWABZ010000170.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6569_length_3342_cov_2.837542"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          697 a          918 c          873 g          854 t
ORIGIN      
        1 caccgacctc acgcttatca ggcgtgcgct ctaaccagct gagctatagg cccatgtcat
       61 gggacttgct tagtataaca taccgcagga aattgtcaag tgaaaaattg gtgggcctag
      121 gtggacttga accaccgacc tcacgcttat caggcgtgcg ctctaaccag ctgagctata
      181 ggcccatttc ctaacgactc gtttagtata ccacagatac aggacgtgtc aaggaaattt
      241 ttccaatttc ttacatcttt ttacttatac tgttccatca gtttcttaaa ggcagcaaag
      301 gaccgtttaa tcatatccgg atcgacgcag tacgatacgc ggacatagcc agggcagcca
      361 aagctgtcgg ccggtacgat gagcagattc agcttcttgg ccttgtcgga gaaagccttg
      421 gcatcggctt caggcgattt gacgaagagg tagaaagcgc cgctcggata gacgcagtcg
      481 tagcccattt ctcttaaacc attatataag agcttgcggt tttcgtcgta aatgttcatg
      541 tcgccagtct ggcccaggca tttgaggacg acgtcctgga aaagatgagg tgcgcagaca
      601 aagcccatgg aacgggcggc accgcagaca gctgtataga cttcctgggc atcggcagcg
      661 cagtccggaa ccaggacata gccgatacgt tcgcccggca ggctcaggga tttactgtac
      721 gaatagcaga tgatcgtgtc tttgtagaat ttcgggacat agaggatggg aaggccatcg
      781 taaatgattt cccggtacgg ttcatctgaa atgatataga taggatggcc gatttcctgg
      841 gacttccgcg tcagcagggc cgccaatttt tcataggtat cagccgagta gacgacgcca
      901 gacgggttat tcggcgaatt gatgatgatt gcccgtgtat gcggtgtaat ggccttttcc
      961 agggcgtcca gagaaatctg gaagtgttcc gtatccggcg gcaggacgac ggctttggca
     1021 ttggcattgt ggacaaagac cgtatattcc gggaagaacg gcgcaatgag gatgattttg
     1081 tcatccgggc cttcgacgag ggctttgagg ctgatggtca gcgaagctgc agccccgcag
     1141 gtcatgtaga aattatcggc ccgtacctgg gcgtcataag tctggttcat gtaatcggcc
     1201 aggccctggc ggacagacgc atcaccggaa gctgccgtat agccgtggat ggccacggat
     1261 tcccgcgttt ccaagatgtc ttcaatggct tctttgacac aggccggggc cgggacggtc
     1321 ggattgccga tgctgaaatc gaagacattt tccttaccga cgatagccgc ttgttcctgt
     1381 ccataagcga agaggtcacg gatgaccgaa gcctgggagc ccaaggcgta catttctttt
     1441 gatgccataa taacaactcc ttttaatata tattgattag tttgttatta ttataacacc
     1501 aatatgaaaa ggaaaacagc ctatgctata atagatatga tttcaacttc ctgaaaggag
     1561 aatttgcatg aagaaactga gtccttatgt atacctattc gctacgggcc atttttccgt
     1621 agactgggcg caaggcgcca ttccggccct cctgccctag ttcatctcgt cgtgcaacct
     1681 gaactatcag gacgcagcga cgctcatctt tgccaacatg ctcttatcat cggtttccca
     1741 gcctattttc ggttattatt ccgacaaggt gtccaagccc tggttcgctc cgctcggccc
     1801 catcctctgc ggcctctgcc tgacgatgct ggcctttacg cagaactact ggctcatctt
     1861 cctctgctcc atgttcagcg gcttcggctc ttccatctat catcccgaag cggcccgcat
     1921 ggtcaataac atcgccggcg accgcaaggg acaggctatg gggacctttt ccgtcggcgg
     1981 caatgccggc ttcgccatcg gccccatggt tgccggagcc tgtgcctatg ccttcgacat
     2041 ccacggtctg gtcgtcttcg gcatcgtcaa cgccctcatc gccttcgtcc tctaccatca
     2101 gatgccgcac atcttatctc tggtcagcga agcggcgacg gccgaaaaga aagcccatcc
     2161 cggggaagtc cggaccaacg actggagtgc cttcgcccgc ctgtccgtcg tcatcttcgt
     2221 tcgttccatc ggctttacgg tctgcaatac cttcattccc ctctactgga tccacgtcct
     2281 ccatgcctcg ccggcaacgg gcagtctggc cctgtccatc ctcttttcca tgggcgtcgt
     2341 catcaccttc atcggcggcg tcctggcaga ccgcttcggc ttcctccgcg tccttcgcgg
     2401 ctctatggtg atcatgattc cgtccatgtt cttcctggtc aacagtacgg acatcgtcac
     2461 ggcgacgctg ctgctccttc ccgtcgcctt ctcgctcttc gccgcctaca gccccatcgt
     2521 cgtcctcggc cagacttacc tcggcaagaa cgtcggcttc gcttctggcg tcaccctggg
     2581 cctgacgacg accatcggcg gcctcatctc gcccatcgtt ggctggggtg ccgaccagtg
     2641 gggcatctcc ccggccctgc aatgcctctg gatcagcgcc atcttcggcg ccatcttcgc
     2701 cttcctcatc cccgtgccca aagcctggcg gcattgattg taggggttag tggctagtgg
     2761 ttagtggtta gtggctaact gctgaccgct gactgctgca aagcaaaaat cccctgcatg
     2821 atggtggaaa ccttcatgca ggggatttta tttgcataga ttactgtgct tttgcgactt
     2881 ctacgagttt tgcgaaagca gctgcgtcgt tgacagcgag gtcagcgagc attttgcggt
     2941 ttacgatgac gccagctttg gtcaggccgc agatgaagcg gctgtagctg aggccgttag
     3001 cacgggttgc ggcgttgata cgggcaatcc acaatttacg gaattcgcgt ttcttggcgc
     3061 ggcggtcacg acgagcgtag tagagcgctt tcatgacgct ttcgttagct tttttgaaga
     3121 gacggctgcg ggtaccgcgg tagcctttag ccatttttaa gattttttta tgacgagcgt
     3181 gtgctgtaac gccaacttta attcttgcca ttagtctatt cctccgaatc tatatcaaaa
     3241 agtatattac gctattagat gaacgggcac atcttgcgga cggcttcggt catggtcttg
     3301 gaaacgagcg tagcttttct caggttacgt ttacgtttcg gg
//
LOCUS       DAWABZ010000171         3326 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_6601_length_3326_cov_4.016509, whole genome shotgun sequence.
ACCESSION   DAWABZ010000171 DAWABZ010000000
VERSION     DAWABZ010000171.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6601_length_3326_cov_4.016509"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          802 a          884 c          940 g          700 t
ORIGIN      
        1 cagactcaaa ctgacaacga cgcttcggga aaccctcagc gcgtagcggg acgccctctg
       61 ggcgtcccct acggcatcgc atacaggcga tgaaaaatta cgcggctata tgccgcggtc
      121 gtaggggccg tcccaacggg cggcccgccg cgagatttgc tcacagccca ttcgattatg
      181 gtggtatgtt tttacgaacc acgagccacg aaccacgagc cactgattct gtgttatact
      241 atggtgtata cggtttatca tgaataaagg gggtctcctc atgaagtgtg ttgttttgct
      301 cagtggcggc gtcgattcta cgacgtgtct ggccatggct ttgacgaaat ataaaaagga
      361 agacgtcttg gcgttgacgg cttattatgg ccagaaacat gccaaggaat tggaagcggc
      421 ccgcaaggtg gcggcttact acggggtgca gcagcaggag gtcaatttgt ctcaggcttt
      481 ttctctgagc gattgttcgc tcctgcaggg cagtcataag gcgattgccc atgaatcgta
      541 tgcagatcag ctggccaaga agagcggcga agggacggtt gatacgtatg tgcctttccg
      601 caacggtctg ttcttgtcgt ttgccgcagc cgtcgccgtc agcgtcggcg cggaagtcat
      661 ttattacggt gcccatgccg atgatgccgc tggccgggcg tatccggact gcacaccgga
      721 atttgaaacg gccatgaaca cggccattta tgaaggttct ggccggacct gccacttgga
      781 agcgcctttg ttatcgtgga acaaagcggg cgtcgtcaaa gaaggcctgc gcctgggcgc
      841 gccttatgaa ttgacctgga gctgctatga aggcggcgac aagccctgtg gtgtctgcgg
      901 gacctgccgc gaccgggcgg ctgcctttgc tgccaatggg gtcaaggacc cggcattgtc
      961 ataaaacagg gagtcaggga ggagacatat atatggaata taaggtaaag acgcggctga
     1021 cgacgatggt actgttggct ctcatggcct ttgggtccgt ccaggccagc gatgctgtgt
     1081 ccgaaaaaga taatttttat caggctgtca atggcaagac gttagcagct cagcaaatcc
     1141 ggccgacgga agcttcttgg tcctggtttt ctgaacggtc tttagaaaat aagaaagccc
     1201 tgggcaagga actggaaacg gtcgccgcca agcagggaac ttatgccaag gggacgccgg
     1261 aacagaaaat cgccgacctc tacgcctgcg ccatcgacaa taaacagcgc aacgccacgg
     1321 cccgcgccca tctgcaggaa ctgacctgtc ccattgaaaa agcccagacc ctgccggaat
     1381 tgacggcggc cttgcaggct gtcagtgcca agaccgggac ggatatcttc gtaggctaca
     1441 ccgtcgaccg cctgccgacg ggcctgcgtt atgtgccgcg tatcttgacg gtgacgccgt
     1501 cctttacgaa ggatgaattg gaaaaagaac cccagcccgg agcctggaag gcttatcggg
     1561 aatacgtcgc ccatatcctc gaagaagccg gagaagcgcc ggaccaggcg gcggctcaca
     1621 gcgaagccat cttcgccatg gaaaagggcc tggggccaca cttgctgacg agcgaacagc
     1681 gcaatgacgt gactgtccag aaccgcctga tgtcgcgggg gaatctggaa aaactcatgc
     1741 ccaatatggg cggcaagacg gtcctgacca acctgggcct gaataaagaa aaacaattct
     1801 tcttatcgaa tccggagtat ttacagcagt tcgacgccct ctatgtgcct aagaatctcg
     1861 acctgctcaa gagctatgct gtctatcagg tctatagcgg ctttgcgccc agtgcggata
     1921 tcaaattgcg ggatctgcag cgcaattatg ccctccagcg cttcggcatc gcccaggcgc
     1981 gggatgacaa ggaaacggcg agccgcatgg tccagaacat gctgtcttat gaattcggcc
     2041 agatttatat gaagaatcac tgtacggcgg ccatcatcaa cgatgtgaag gccatgatca
     2101 atgaaatccg cgacgtctat cagcagcggc tggaagccaa tgactggctc agtcaggcga
     2161 cgcgggccaa agccgtcgac aagctcagcc acctgcgcgt ctttgtcggc ggtccggctg
     2221 aagatgacaa acctatcatc gaaagtatgc ctgacgtcat tgctccacag gatggcggcg
     2281 atttgctggc caatgtcatg cacaacggcc tcctggaaaa acagcaggtc catgcgctat
     2341 tgggaacgaa ttttaatccc gataaatggt atgcctttga cccgcaagat gtcaatgccg
     2401 cttatatccc ggaaaataac agcatcacca tcccggccgg tatcctgcag ccgccgttct
     2461 acgatgccaa agcttcacgc ggtgccaatc tgggcggcat cggcgtcgtc attggccatg
     2521 agatttccca tgcttttgac ccgaatggca gcaagtacga cagcgaaggc cgcttgaaga
     2581 actggtggaa ggcaaaagac tatgaagctt tccagaaatt atcggctgct ttcggtcctt
     2641 attacgatcg ctataccctc ggcaaggggc tccatgaaaa tggcaagctc gtcagcaacg
     2701 aagccattgc cgactgcggc ggcctgtcgg ttgcgacgga actggcccat gacgaccaag
     2761 ggatgctgcg cgacttatac cgcacctttg ccacggtatt tgcgaccaag atgaccgacc
     2821 agctgctgct ctacctggtc cagaatgatc cccatccgac tggcgaagcc cgcgtcaacg
     2881 gcgccttgtc gtcgacgaat ggcttctata cggcctatgg catccagccc ggcgacggca
     2941 tgtatgtcga accgcagaag cgcgtccatt tgtggtaaaa tcttcgtggc tagtggctag
     3001 tggctagtgg ctagtggcta gtggctagtg gctagtggtt cgtaaggccg tcctgctgcg
     3061 gcggcctgcg gcctgtgtcc tcataaagta tttacacaac taatccttgc attatcgtgg
     3121 ggaatacggt acaatcgcag tgagcaatag ccgtatattg cactagtggg ataaaaaacg
     3181 taccactagg aacagaaacg tcaatcgtta cgtgtacgtg agtagaaaag gagaaatctg
     3241 tatgctgaaa aaaacaaaga tcgtctgcac cttaggcccc agttccaata ccccggaaat
     3301 tatcgaaaag atgattaaag ccggca
//
LOCUS       DAWABZ010000172         3322 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_6612_length_3322_cov_2.850015, whole genome shotgun sequence.
ACCESSION   DAWABZ010000172 DAWABZ010000000
VERSION     DAWABZ010000172.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6612_length_3322_cov_2.850015"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          764 a          857 c          948 g          753 t
ORIGIN      
        1 ggttaacagc cgattgctct accaactgag ctattgagga atatcacttc aagctcgttc
       61 cgaatcggaa caattaatat tatacttgaa gagatagaaa aatgcaagtc ttttttacga
      121 atttttttga tgactttctc cggccctgcc caggaaatgg aaataggccg taaagcaata
      181 cacggcagcg actaagccga tggctgcgcc ggcatagccg atatacggta gcaggccatg
      241 ggccgtgacc tgcccgccaa tccaggtccc gccgccgata ccgacgttga agatgcctga
      301 aaagatggac atggccaccg gggctgcccc catcgggacg tggcggatga gttcggcctg
      361 gaaggtgacg ttgaacagcg tcgacgcaca gccgacgacc aggcaggcgg cgaccatcgt
      421 ccctgccctg cccgcagcgg cctgccacag gaggaacggg atgaccaggc ccaacagacc
      481 agcccggatg acgccatagc ggaaatgatt gtaaaaccgg gaaaagacga aactccccag
      541 gaggccgctg acgccaagga gcatgagcgt cgacgtgatg ccctggtcgc taaaggcagc
      601 aacggtcttg agaaagggct cgatatagct gtacgctgta aaataggccg tcgcgaacag
      661 gacggacagg atatagacgc tgatgacgac gggattcttg agcagcttgg gcaattcgtc
      721 gcgggaaaaa gaggcgtcgc tggccagctt gggaaagaca aaggccaaat agatgagcac
      781 ggctaaggcc gttaccgtaa tgaccagaaa agtcatgcgc cagccggcat agaggccgat
      841 gacccgtccc aggggcaggc cgaagatcat ggcgacagcc gagccagtga cgatcatgct
      901 caaggccagg ggccgatgag tccgcgtgac caggcgcgtc gccatcgggg ccgcaatgga
      961 ccagaaaata gaatgggcgc aggcgacgcc gatgcgggct gccatgagca tgggaaaatt
     1021 cagggccagc cccgatgcca gctgtcccag gacaaagagc cccatcgtcc ccagcaggag
     1081 gcgccgcatc tccatctggc agacgaggag catgagcggc agggacagca gggccacgac
     1141 ccaggcgtag acagtgatca taatgcccgt ctgggactcg gtcatcgcga aggactgaga
     1201 aatgtccatg agcaggccga taggcatgaa ttccgacgta ttgaagatga acgccgacac
     1261 agtcaggccc aggaggggca atacatattt cagaggaata gcgcgaaaca tgattcattc
     1321 ttcctttgca tagatttaga acgattccat cgtacgctgt ccccgggaaa aagtcaagag
     1381 ggcaaaaaaa aggcttgtcg ttatacgaca agccttttta agttgttagt ggttagcaga
     1441 tggctttaaa tttaaaggcc gtctctgcaa actacacact acaacctaat cttacatcat
     1501 gccgggcata ccgccgccca tgccagccgg ggctgccgga gccggttccg gtttgtcagt
     1561 gaccagggtt tccgtcgtga ggacgagggc tgcgatggaa gctgcgttct ggagagctgt
     1621 acgggttact ttagccgggt cgacgatacc tgctttgacc atgtcaacgt attcttcttt
     1681 gagggcgttg aagcctacgc cgtcttcgga agctttgact ttagcgacga tgaccgagcc
     1741 ttcgaggcca gcgttttcag cgatctggcg gagcggagct tcaatagcgc gtttaaccag
     1801 gttgataccg gtctgtacgt cgccgtcttc atcgaattcg tcgagagccg ggaggatgtc
     1861 gatgagggtc gtgccgccgc cggctacaat accttcttca acagctgcac gcgtagcgtt
     1921 gagggcatct tcgagacgat atttcttatc tttcaattcg acttccgtag cagcgccgac
     1981 ttcgatgaca gctacgccgc cggacatctt agccaaacgt tcctgcaatt tttctttatc
     2041 gaaatcagac gtcgtttccg caatctgagc tttgatctga gcgacacggt ctttgatggc
     2101 ctggggatcg ccgtggcctt cgacgatagt cgtttcacct ttggttacgc ggacctgacg
     2161 ggcagtgccg aggtcttcca tggtaacgct gtcgagttta cggccgacat cttccgtgat
     2221 gaccgtagca cccgtgaggg ttgcgatatc ctggagcatt gctttgcggc ggtcgccgaa
     2281 gcccggagct ttgacagcga cggctttgaa ggtgccacgg agtttgttga cgaccaaggt
     2341 agccaatgct tcgccttcga catcttcagc gatgatgagg agttctttac cagcctggac
     2401 gactttttcc aggaccggga gcatttcctg gatagaagcg atcttgcggt ccgtaatgag
     2461 gatgtacggt tcgctcatga cggcttccat tttatccggg tccgttacca tgtatgggga
     2521 aatgtagccg cggtcgaact gcatgccttc gacgacagag agctgtgtgc ccatggtctt
     2581 cgattcttcg acagtgatga caccatcttt gccgactttt tccatagcgt cagcgatgag
     2641 gccgccgact tcttcgtcac ctgccgaaat ggaagcgacc tgagcgatgg aagccttgtc
     2701 ggatacggcg atggaacgtt tcttgatttc ttcgacgagt ttagcgacgg ctttttcgat
     2761 accgcgtttg aggatcatcg ggttagcgcc ggctgcgacg ttgcgcatgc cttcctgaat
     2821 catggcctgg gccaggatag tagctgtcgt cgtgccgtca ccagcgacgt cgttggtctt
     2881 ggttgcgact tctttgacga gctgagcgcc catgttttca aagggatctt ccaattcgat
     2941 atcgcgggca atggtgacac catcgttggt gatggtcgga gcgccgaatt tcttgtcgag
     3001 aacgacgttg cggcctttag gccctaaggt aacttttacg gcatttgcca aagcgtctac
     3061 gcctttgccg agagcgcggc gtgcatcttc attgaacaaa atctgtttag ccatgttgaa
     3121 cacactccta tgtgaaagtt aagtattttt tagtttgatt cgaaatgatt tattagatga
     3181 tagccaagat gtcgcgttcg ctgacgatca aatatttagt gccttcgaac ttgacttctg
     3241 tgccggcata tttggagaaa ataatcttgt cgcctacttt gacatccaaa gcaacgcgtt
     3301 taccgtcgtc ctgcagtttg cc
//
LOCUS       DAWABZ010000173         3268 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_6721_length_3268_cov_2.228758, whole genome shotgun sequence.
ACCESSION   DAWABZ010000173 DAWABZ010000000
VERSION     DAWABZ010000173.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6721_length_3268_cov_2.228758"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1716..1809
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          788 a          878 c          667 g          841 t
ORIGIN      
        1 ccgtattaga agcggctttt ctcaaatgtt tagttaggcc tgcaccgtag aagatattgt
       61 cccctaaaat catagcgcaa gattctccat tgatgaattc ttcccctaaa ataaaagcct
      121 gggccaatcc atccggtgac ggctgcacct tataactcag atggatacca tagcgcgacc
      181 catcccccag gagccgttca aaattcggca aatccgtcgg cgtcgaaata atcaaaatat
      241 cccgaatccc agccaacatc aacgtcgaca aaggataata aatcatcggc ttatcataca
      301 ccggtaacaa ctgtttagac gtcaccatcg tcagcggata caaccgcgtc ccgctgcccc
      361 cagctaatac aatccctttc aaaaaaatca ctcctcacag taaaattcaa tacctaatta
      421 taccaaaaaa gaacctgaca tacaaagtta tgaataactt tatacgacag gctcattttt
      481 cttactttag aaccatccct acaccatggt aatatcttta tactgcggct tcacgatcca
      541 ctggccctga tgatccatga tgccccagcc tgtctttgtc ttgaccggcg tcaggccgtt
      601 gcggaagcct ttgacgtcct ggatctggcc ggcggcagca cggtactgga cctgaccgtt
      661 cctgtcgaca atggcatact ctttcttgtc gttccagcac caggtataat cgtcgaggag
      721 gaaagccatc catttcacac tggccgggaa ttccgcaaca aaggcccctg tttcctgatt
      781 gatgagcata tttttatcgc ctttcgtcag caaaaggcgc tgtgatccca ggaactgtac
      841 gccgtcatag gcaaaatcga cgacctgtgc cccgtcgacc atactgaaga cgccccattt
      901 cccgttttct ttatctttca aagaaacgaa gccgtcgaca tcatccaaaa gcccatcgtc
      961 gagagtgtaa ctccccggcg caatcaggta ctgcccctta cggttgacaa aacccaattt
     1021 ccctttattg cggacgaagg tgccaaattc cgtcatcgga taaacggcat catttttgct
     1081 gtcgataaca atattgccga cgcggtcgag atagccccgc ttcacgccgt cataggtcaa
     1141 aggcgccact tcgtcatcgt aatagacgga atgcgtcgac gagccaatca gcgcgccggc
     1201 gaggaaaccg cccaggttaa aatggctgac cctgcgctgg tattccgcca gaccgtggtg
     1261 atagggcaaa atgacatcat acgacgcagc gaactggacc cggccgcttt catcaatggc
     1321 cactttctct cccttattgt ccttgacgaa agccaggcct tcgctgaagt cagccagcac
     1381 agccttatac gtcggtgcca ctacgatgtt cccgttcttg tcctgaatgc cataacggcc
     1441 ctgatccttg aagaaatgga ccgacgcggc ttctttctcc ggccaaacca ccggtgcacc
     1501 ctgcttatcg acataagccg tccctctctt cccttccgtc ataaacacgc ctttaccggc
     1561 ataggacagg gacttatacg ccggcgtcat gagccatttt ccatccgtgc ccatcaggcc
     1621 ccacttatct gtaccgtcct tgaccgcagc caggacacca tccttataat tggcatcgta
     1681 ttcctctttg acctgtgaag aaatcgtctc catccnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnng caaatgcctg gccatcgccg gaaagacaac atcccccagc gacgaaggga
     1861 ggctgacact ccagcccgaa tgagacgccg actcacgcgt cgacgaatgc gactccgtct
     1921 gcgtcaccgt cgtcgtatgc acctcacgat gcgcctgttc tgcaagcgaa gccggctcag
     1981 cggcctgtac cgccaaaatg gataaagaag tgcataaaaa acctgctaaa ataaatcgtt
     2041 tcaatgaaaa atggttgtac atgtcgcgcc cctttcaatc tccaaacaga acgacgttgc
     2101 cggcgctacg ttatatcaat ccaacaattg atgtagctgc gtcaaaaatt cattaaagaa
     2161 tttatcatct acagcagttt acaacttttt gaacaaataa atatcacgta agttgtcgat
     2221 ttgagtttgc taccgcgccg cggcatctga gtccgaggcg gcctgcgaaa ctacaagcag
     2281 cctctccggc cgttggacta cctctcttat caggaactgc ttatttcttc tttttgcgat
     2341 ggacgatgag ggcttcgtcg gcccagtcga agaagtacgg ttcggttgcg taaatggcat
     2401 agttcgtcag ataggcagtg atgagttctt catcggaaaa tggggcgtat ggcgggttgt
     2461 caaggtaggg ctggaagatg ctctggtcac cctttttaat ggaacggatg agctttttcg
     2521 taatagggta ttcctgcttc aaggtttcca agtatgattt cgcctcgggc caatcgccga
     2581 gtttgaagta caacaaggct aaggccaaga gataaaagga actcttttgt tcgtattttc
     2641 cccgtttata cagtttcaaa gccttcattt cattgcacat tttagcatac agcggcatca
     2701 gcagaaaacg gattcccaag ttgtcgtttt gattgaggcg aagcatttct tcccctatct
     2761 ggatggcttt gttgtagata ccacattcag tcagatccga tagatattct tgaagcagca
     2821 tcatataagg ccgtgtttca aagtccagcc aaaaaccacc tttgctacgc tggtaaattt
     2881 cctgatcttt taaccgtttt tcttcggctg caatcatcca gggggccttt cgaatccaat
     2941 tcagcttggg ccagtttgac ttccacatta tccggctcca gttcggctgc tttagccaaa
     3001 tattcccgtt tgtctttttt tcggctcgcc tgctcagcta agtctaaata atcatagaca
     3061 tcggctggta ctgtatcgtc ctgcccattc tcctgcaaaa agtcctgcaa gtgttggaca
     3121 gcctcattat gctgcatcat gaattcttca ataatctgct gcatctcttc ttcagttgct
     3181 gttttttgct ctacggcttc tttcgctgca ttcagtgctt tttcataaac tttccaggtt
     3241 tcttctgtat agtcactttc tttcctgc
//
LOCUS       DAWABZ010000174         3243 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_6773_length_3243_cov_2.868883, whole genome shotgun sequence.
ACCESSION   DAWABZ010000174 DAWABZ010000000
VERSION     DAWABZ010000174.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6773_length_3243_cov_2.868883"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          706 a          711 c          861 g          965 t
ORIGIN      
        1 cccatatata atgattccaa tagattcgtc ttgccctggc cattcttccc atagaggagg
       61 atgatggcct tgggaaaggt aatcatttca tcatcataat tccggaaacg gtggagccgg
      121 accgattcaa tcatcataat tattcacctt taatcaaata tgtaccgata cctttaattt
      181 caactttgtc tccaggatag agttttttgc gtttcgcagt gcataaaatt ccatttacaa
      241 aaatcttttt ttcgtccagc agaaagcgga tttcaccgcc gctctgtaaa atactggccc
      301 atttcaagaa ttggtcgagc tggatcatcg gtgtcgtaat gtttactgtt tccattattt
      361 tgtcctcata ggcgttacaa caaatacaca ggaagtatct tctttatcct ggaccagcat
      421 ggggctgttt tccttggatt tcaagatgac ttccgaatca tggcaatgct tcaagatgtc
      481 gaggatgaat ttcccattaa aggaaatatc caggccctgg ccttccaaat ggcaggggac
      541 gtagtcttcg accgtaccgt agtcaggatg ctggctcatg agtttgatct ggtcgtcggc
      601 gacgatgaag ttgatgacat tgtactgggc atctttggcg acgatagaag cgcgttcgac
      661 agcgcccgtg aattcggccc ggtccaccgt gacgatgcac ttgaagtcat tggggatgac
      721 gcgccgataa ttggggaatt ccccttcgat gagccgcgtc cggatgtaga cattgccgaa
      781 ctgtacggcc aggtaattgc ggactgccgt gatttcgacg gtttccggga tatctgtcgg
      841 cagctggcgg gaaatttctg ataacaatcg gctggggata acggcctgta agggattatc
      901 cacaggcgca tcgagggtag ccgtcttcaa tgccaggcgg tgcgtgtcag tggcgaccat
      961 ggtcatctga tccccctgga cgtccaggaa ggtccccatg aaaattgggc gggccacatc
     1021 agtagcgcag gcataggacg tattgtcgat gaggtccttc atgacgacgc tcgtcgtcgt
     1081 aaagctggca tagccgtttt caacgatttc gacttctgtg aaatcatcgg gattcatggt
     1141 cagacattca aacttgagtt tgccgccttt gatgagcagc tgtgtatcgg catcttcctt
     1201 gatgatttcg atttcatcac taggcaggcg gcgggtcaat tccattaaat aaggattggc
     1261 aataaaagct tcaccgcctt cttcaatgat accgggaacg gtgatcttaa tgcccatatc
     1321 gtagtcattg gcctggaact cgatgatacc atggagggct ttgataagca agccgttttc
     1381 agaagatata ttgttgcttt tgttctgggc gactttctgc acagcctgca ggcctttgtt
     1441 cagatcttct tttttcagtt ttagtctcaa tggagtatcc tcttttctgc gctgaataaa
     1501 ttaattaaat gtgaattaaa aaatagtagt cttagtaata ggggctgtga atttgtggat
     1561 aagtggaaaa agacagcgat tttaagctgt ccacagctgt ggataacgtg tggacaaatc
     1621 gtcatttttt atccacattt ccactgtgga tttctttttt tgacttatcc acaatgtatg
     1681 tacaatttta tcagcctagt tatccacacc gcggatttca ttcatcatct tttccagatg
     1741 ttctttgaaa tggctgtctt tgtctatttt cttccgaatc tgcgagcagg cgtgcatgac
     1801 tgtcgtatga tcgcggccgt tgaaggctgt cccgatgtga ggatacgtat ttcctgtcag
     1861 ttcgcggcac atatacatgg caatctgccg cggcagggcg atgtctttgg tctttttctt
     1921 gcccatgagc tctttataat tgatgttata gtgttgacag atgtacgtcg tgatttcgtc
     1981 aatggtgata tatttctttt catcctgggc gtcggtaatg taatggatgg cgtgttttac
     2041 taaaggcagg gtgacgttgc tcttggactg cgatgcttcc aggaggacgc ggatgaaggc
     2101 gccgtgcagt tttcggatgt tttcgctgac attggctgcg atgtaattga gggcatccag
     2161 gtcgcaggtg atattttctt tttctgccca ggcccggagg atagccgtgc gcgtttctaa
     2221 atcaggcggc tcgatttgcg tgaccatgcc gttttcaaag cgggaacgca ggcgttcttc
     2281 cagtttcttg atgtcccgtg gcggacggtc gctggtgaag accatctgtt ttttgtcctg
     2341 atagagcttg ttgaaggtgt ggaagaattc ttcttttgaa ctttctttat ccccacggcc
     2401 caggaactgg atgtcgtcta cgaagaggac atcgacattg cggtaacggc ggcggaaact
     2461 ttccgtgttt ttgtcctgga tacactggat gaattcgttg acgaagtctt cactggtgat
     2521 gcacaacagg cgcatgttag ggaagttctt cagcaattcg tggccgatgg cgtgcatcag
     2581 gtgggtcttg cccaagccgc tgccgccgta gatgtagaag gggttccatt tctgggccgg
     2641 tgcggctgca atggacttgg cgacagctgt ggcgacgcgg ttgctattgc cgacgatgaa
     2701 gttatcaaag gtataatctt tatttaaatt attattataa gggatttcat caggcgtgac
     2761 ggggacggct tctttgacga cgggcatggg ggctgacgga ccgtcactga tgggcaattc
     2821 aggctggacc cattctgtct gtcctgacgg gacggactgc gcgtctccgg ccggggcagc
     2881 ggctgccggt acgggaggcg ctgccggctt tttcggcagg gtgatcgttt cagcgacggc
     2941 ttctggctgg ctttcgggag cgggcttctt ttcctggctc gtaatcagct ggacccttgt
     3001 cgggcggccg atggcgtcct gggcgacggt ttccaggata tccgtgtatt ttttgctgac
     3061 gtagtcgcgg agaaaattcg tcattgtgtc taatatgagg agattttctt cttcggaata
     3121 ggagtaggga atgatgttgc cttcaaacca gttggtaaaa atctctttcg gcaagacttg
     3181 tttcaggtta gccagcattt tttcccataa gctgagcagg tccataataa tccttccaat
     3241 ctg
//
LOCUS       DAWABZ010000175         3185 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_6893_length_3185_cov_3.097444, whole genome shotgun sequence.
ACCESSION   DAWABZ010000175 DAWABZ010000000
VERSION     DAWABZ010000175.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6893_length_3185_cov_3.097444"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          760 a          889 c          893 g          643 t
ORIGIN      
        1 aagaacaatg cgaacaacat caagccaata gcaatcgttc tgtttccttt atttgctccc
       61 atatgacact cctcctatct ttttaaattt tagattttgt caagaaatca taaggacaga
      121 aaatgtaata aattaaataa tttaatttgt gagtttattc tatcataaaa aaggaaaaaa
      181 atcatgaatt tttcgcaagt ttaatctaat tctaatgttt ctctaatctt tctttaatat
      241 tttagtaaaa atcagaactt acttaatttt tcccgcattt tttccacgaa tctttcattt
      301 tcaaaacgag gccgcacggc attgcgcccg tatgccgccg tcaggcccgg ataattggta
      361 caatcgcgga tcaaaatccc gtcagcccgc aaggcgtctg ccaggtctgc cgtccgcttc
      421 ccttctcccg taaggcgcca gaagatgaaa ttgacacaag gccgatagac gtgaagaccc
      481 gacagggccg tcatctgttc atagagccag tctttctcct tctggaccaa ttggcgcgaa
      541 gcctgctgat aggcccggtc ccggagtccg gcgacgccgg catactgagc caggacattg
      601 acattccagc cgtctttatg cccctccatg aggccgcgcc gcaacggcga cgtgacacca
      661 aaacccaggc gcaggcccgg cagagcgtag aatttcgtca gcgaccggat gacgaacagg
      721 ttatcccggt ccccggcctg gtccaggacg gaataggctt cgtcagaggg aaggaaatcg
      781 aggaacgatt catcgacgag gatatctgtt cccttgcggg cgccgacttc ggccagggcc
      841 aggacttcat cggccgtcgc cagggtgccc gtaggattgt tgggattgcc gatgacgatg
      901 cagtccacct ggccgcaggc ccggcgcagc ccttcccagg gaaaggcaaa gtcatcgtct
      961 tctttcaggt actcatagcc gataacggcc ccaacagccc gtgccgcccg ttcgtattca
     1021 ctgaaggaag ggacgggcag caggacccgc tgaggccgga aagtatgcat atagagataa
     1081 aaaagttcgg ctgcgccatt gcccagcagg atagtcccgc ctggtacgcc gtaggcccct
     1141 tctaaggcct ggcgcagcgc cgtcccggcc ggatccggat aatggacgac ggcgtcgaga
     1201 tgggccgcca gggcctggcg gatgctctcc ggcagtccga gaggattgat attggcactg
     1261 aagtccagcc acggaccgcc atccggtgcg ggctgataga tattgccgcc atgttcaaat
     1321 cgttttatca tggaatcacc tgttttctat ggaaataccg ccgctgcgca aatggaccgt
     1381 ttctgtatag gtccaatgcg aaaactgccg gctcagtgaa gcaatgaccc cctccctgtc
     1441 cgcctgactg gcagactggc agaaaaagac gccggccaca gtcccgctgt gggctacgtt
     1501 gacgccggcg acagccggat gacggcgggc agcccggaaa aaggccggaa aatccggttt
     1561 cggcagccag ggttgattgg ccagggcact gagggtcgcc gcctgtccga tctggcggtc
     1621 tgtcagcgac gacgacaaga gctgccagcc caggaagaga gccgccgcat caggccggac
     1681 acggtctggc cgggcctgat ggaaagctac ggtatcgacg gtcccaccgg catcaaagat
     1741 ggcaatagtt aagggtggta cgtgcggaaa gacatggagg agctcccccg tttccggctg
     1801 tatcactgcc aggccctgac aaaaaatgcc gtctgtcggc tcgatggaag ccgccagacg
     1861 gccaatgtcc ccttcagaaa gatgcacctg gttcacggct gctacggctg ccaggacagc
     1921 gcagatatcg gccgaactgg aagccatgcc cttgccggaa ggcaattccg acgtcaggaa
     1981 aaaatcatga gccaggctgt cgcactggca ataggcctgt cccagcgcca gggcacggca
     2041 tgccttggcc ccgccgccga cgagccgtcc cgtcccgggg cggacgacga cggtactgta
     2101 gcggtcgatg ggacacgtaa ccaagaaggg ccttccctgc cagaagccct gcatcacttc
     2161 cccgcacgaa ccgggaacgc ggacaatcag ctccataagc cgtagagccc taaatgaagg
     2221 agcgccgtaa tgacaccggc tgccagggat acgctgtaca tgagatacat ggccttgcgg
     2281 atatactgcg gttccagttc atggtcggca tcgcccatat attcgcggaa tgtatccttg
     2341 ccgaaataac ggttatgccc gcctaaacgg atgcccaagg ccccggctac ggtcgcttcg
     2401 gcatagccgc cgttcgggct gggatgctta gaggcatcac ggcgcatgat gcgccaggcc
     2461 cctttggcat catagcccag gcaggcggca gccgcaatga acagcagggc gcagatccgg
     2521 gccgggatgt agttgaggac gtcatcgacg cgggctgcaa cgcggccgaa atagatgtat
     2581 ttatcggttt tatagccaat catggaatcg agggtattgg ccgcccgata gacgacagcc
     2641 aggggcaggc cgccgatggc aaagaagaac agcggcgaca cgatgccgtc aatggtattt
     2701 tcggcaatcg tttccactgt agcccgcgtc acttccgggc tgtcgagatg gtccgtatcg
     2761 cgaccgacga tcttgccgac cttcaactgg gcaatgcgca gatgatgacg gacgagatag
     2821 cgatagactt cccgaccggc cttggccagg ctgcgggggc agatcatgaa ggataagagg
     2881 atgccctgga tgatataact ccaatgttcg ttaggtaaaa attccaaggc caggaccagg
     2941 gccagggtga tgttgtagac aaacaacagg gtaatgacga cagtcagccc gcctaagatg
     3001 aaatgttccc atttcatcga tttctcatta tagaacagcc gttcgagcag actgatccag
     3061 cgcccgatga gggcgacggg atgcagtttc gtatggggat cgccaatgat gaaatctacg
     3121 ataaaagcgg tgagtacagt aatcatttta ttccttcttc cataatctgc cgcaggcgct
     3181 tcatg
//
LOCUS       DAWABZ010000176         3118 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_7038_length_3118_cov_4.197845, whole genome shotgun sequence.
ACCESSION   DAWABZ010000176 DAWABZ010000000
VERSION     DAWABZ010000176.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7038_length_3118_cov_4.197845"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2800..2809
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          811 a          878 c          807 g          612 t
ORIGIN      
        1 catatagacg acaacgattc ggactgcggg tcttttgctg gccggcagtt attttcagtt
       61 tgtatatgag gtgaaatttg tggacgaaca acaagtagat aaaattgtgc cggtttgtct
      121 ggaaagcgaa atgcagacgt cgtacatcga ctatgccatg agtgtcatca tcactcgtgc
      181 cctgccggat gtacgcgatg ggctaaagcc ggtccatcgg cgtattctct atgccatgca
      241 tgaagcgggc atgacgccga acaagcccta taagaaatcg gcccgtattg tcggcgaagt
      301 tttaggtaaa tatcaccccc atggtgacag ctccgtctat gatgcgaccg tccgcctggc
      361 ccaggatttc tcgacgcgct atcctttagt cgatggtcac ggcaacttcg gttccgtcga
      421 tggtgactcg gctgcggcta tgcgttatac cgaagtccgc atggcgaaga ttaccggcga
      481 aatgctggaa gatatcgaca aggaaacggt cgatttcatg cccaactacg acggctccct
      541 gcaggaaccg acggtcttgc cgtcgaagat cccgaacctc ctggtcaatg gctcggctgg
      601 gatcgccgtc ggcatggcga cgaatattcc gcctcataat ttaggcgaag tcatcgacgg
      661 ctgtgtcctg ctcatcgata accctgaagc cagcctggaa gaaatcatga ccaaaatcaa
      721 aggccctgat ttcccgacgg gtgctaaaat catgggccgc gatggcatca tcaaagccta
      781 ttcgacgggc cggggcagta tcaagatgcg gtcctgtgcg actatcgaac cgatgaataa
      841 agggaagcac cgcatcgtcg tgacggaaat cccctatcag gtcaataaag cgcgggtcat
      901 cgagtccatt gccgacttgt cccgcaacaa ggaaatcgac ggcatcacgg ccctgcgcga
      961 tgaatcggac cgcaagggta tgcgcatcgt cgtcgaactg cgggccgatg tcaatcccga
     1021 tatcgtcctg aataaattgt ataaacacac gcagctccag gaaacgttcg gcgtcatcat
     1081 gctggccctc gtcaacggcc atccgcgcgt cctgacactc aaacagatcc tgaactacta
     1141 tctcgaccat cagaaagatg tcattacccg ccgcagcaag tttgaactcg acaaggccct
     1201 ggcacgggct cacatcttag aaggcctgct catcgccctg gaccacatcg acgaagtcat
     1261 caagacgatc cgcgaatcgg cgacagacga cattgccaaa caatccttga tgagcaagtt
     1321 cggcctcagc gaaaaacagg ccatcgccat cctcgacatg cgcctgcgcc gcctgacggg
     1381 tctggaacga gaaaaaatcg aagaagaata tgctgaattg gaaaaacgca tcgcttacct
     1441 gcgcagcgtc ctggctgacg aacataaagt catggaaatc gtaaagacgg aactcctcga
     1501 tgccaagaag aaatttggcg acgaacggcg cacacagatc gtagccgact cgtcggattt
     1561 ctccatcgaa gacatgatcc ccaacgaacg catggtcctc accttgaccc gccgaggcta
     1621 catgaagcgt atcaacgcca atgtatacca tacgcagaat aaaggcggcc gcggtatcaa
     1681 gggcatgggg acgaaggacg aagacgccgt aaaccacctg ctctatacgt cggcatggaa
     1741 tacagtcctg ttcttcacca acttcggccg cgtctatcgc ctgaaggctt acgaaatccc
     1801 ggaagccaac agccgtaact ccaagggcat tgccgccatc aatatcctgc ccttgtccaa
     1861 tggcgaaaaa gtcaacgccc tcatcgacct cgaccaggtc gacccgtcga tgaacctgtt
     1921 catggtcacc gaaaaagggc tggtaaagaa gacccctctt catgaattca agaacgtccg
     1981 ccgcaacggc ctcatcgcta tttcccttat ggaaggcgac cacctggcca aagtgcgcct
     2041 gaccaacggc gaccagacga tcatcttggc gacgaaactg ggcatggcca tctgcttcaa
     2101 tgaaaacgat gtccgtccta tgggccggaa tacccgcggt gtccgcggca tcacactgtc
     2161 tgaaggcgat atggtcatcg gggctgacgt cctcgaaccg gactgccagg tcctgactgt
     2221 cagcgaagaa ggctttggca agcgcaacaa catcgacgcc tacaaagtcc agctccgcgg
     2281 tggcaaaggc atcaagaact tcaagattac gccgaagacc ggcgacatcg tcggcgtcga
     2341 agtcgtccat gaagggcagg aactgatgct catcacgtcg aatggcatcg tcatccgcac
     2401 ggatatcgaa agcattggca tcaagaaagg ccgcaacaca tcgggcgtca agatccagaa
     2461 actggaaggc gacgacaaag tagccgccct cgataccatt gccatcgaag gtaacgacga
     2521 actcgatacc attgccatcg aaggtaacga cgaagaagaa aaagaacaga cgaaattgtt
     2581 taaataaaaa aagaggcctg cgggcctctt tttttttgcg ccgaccgcag ttcgcaggcc
     2641 gcaggccgcc tcgaattcag atgcggcgtt ccgccgcgac agactcagac tgacaacatc
     2701 gcttcgagta accctcggct cgtagaggag aacccctcca ccgcaagcgg tccccctccc
     2761 cttcgcaggg gcggcttacg ccctaaccgg ggccatcgtn nnnnnnnnnc ccctatgaag
     2821 ggagcctttg acggcccgct gtgaacaatg ggggatatac ttgatcggca tatgtggcgt
     2881 gggtttgcga ccggcgaccg gcggccggcg tcaaggattt gaaaaaatcc ttgacaatct
     2941 ctttacacag tagtatactg aataagctgt cggggcaagg ccctgacaca gcaggagttt
     3001 catcctgccg tggtctgcgc catgcaggcc ggggtgattg aactttgaaa aaagttcaaa
     3061 aacatccttg acaagctctt ctgagtttgt taaaataaac aagtcgtcgc cacccggt
//
LOCUS       DAWABZ010000177         3115 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_7044_length_3115_cov_3.352614, whole genome shotgun sequence.
ACCESSION   DAWABZ010000177 DAWABZ010000000
VERSION     DAWABZ010000177.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7044_length_3115_cov_3.352614"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          643 a          793 c          865 g          814 t
ORIGIN      
        1 tcagctggga gagcgcttgc atggcatgca agaggtcagg agttcgatcc tccttatctc
       61 caccataaag aatctgataa ccacgcggtt tatgagccgc gtggttattt tttattacat
      121 gaaaaatcga agaaggttag cactagggtt agcaataaaa aaaacgacag taaccagcaa
      181 caggaactgt cgttttttta ttcattcttt aaggaaaagg gaccgcgcat ggcagcaaaa
      241 gtgctatgcg acggtccctt tgcatctgac tatgttaccg gagtgggatt ctggtaaacg
      301 aatcaggcaa ccttatctgt ttgggtacgg taataggtgt tttcggcaaa gtctttgacc
      361 gattctacgt cgatcaattc gttttctccc aacggtgtct tagccatggc ttcggccacg
      421 taattgacgt gggtcccggc gacccatacg ccgctgggca ggagttcgac ggcgctgcgc
      481 aggttcttcg agatgtcgtc ggagaagagg tcgcggtgca tgatggcaaa gagggcgact
      541 tttccggctt tgagccgcgg caggacggct tgggagacgt tatctaattt caggttcagg
      601 aaccagacgc cggcaaagac gatatcgtaa tcgtcaaagt tttccggggc ttgttccatg
      661 ggcaggaagt ccgtatcttt cggcaggacc gaacggatgg cttcggctaa ttttttgact
      721 ctacctgtct gtgaggaata caataccagt gctttcatag tttccacgct cctttcacat
      781 ggatgagcat cccttttctc tctggcttta ctatatcata gaaaatagcg gaaaactgtt
      841 gtatttacaa cacctcacag ttcttcaaag acgtgttcgg caatatgaag ggcccgcagc
      901 tgggatgttt ttttgtattg gtgctgttcc atgcgctgga ggatgttggt caggacggtg
      961 acagcttcga cgatgtacgg gcctttattg agcatgacac attcagcccg ttcgcccatg
     1021 gcggcgtcgg tgatttccgc ccgtgacggc aggcccgtct tgaccaggtt ttccaggact
     1081 tgtgtggccc agatgacggg gatatgcgcc gcttcgcaga cccacatgat ttcttcctgg
     1141 agctcagcca ggcgccgata tccgacttcg acggccaggt caccgcgggc gatcatgatg
     1201 cccaagggct gacgggatgc agcctggatg atgatgtccg gcaagtggtc tacggcttct
     1261 ttattttcga ttttggcgac cagggccagg tggtggaacg atttgccgca gcgtttcttc
     1321 aattctttct gcaagagggc tacgtcggct gcggacttca cgaaggaata gccgatggca
     1381 tcggcatgtt ggacgacgaa gtccaggtct tctttgtcct tttccgtcaa aggcgacagg
     1441 ttcatcggcg tctgcgggaa gttcaggctc ttctggttct taattttccc gcctttcggc
     1501 ttggtgtagg taatcttcag gtaggccccc ttttcggtca ggctttcgac gacggcccgg
     1561 attttcccat cgtcgatgag gacgggctgg ccgctctgaa ggttttcaaa gacttccggg
     1621 atgctgcacg acaagacgat atttcccgta taatctgaag gatacgatga aatggcgccg
     1681 gatacgagga agacttgttc tcccggcatg accttggctt cgccatagcg cagcaggact
     1741 gaggcaatgc ggactttcgg accggccagg tccatgtaga ctttgcagtg atgccccgtt
     1801 tcggcttcgg cctggcggat gtaggaaatc atggcatccc agacttcgcg ggaatcgtgg
     1861 gcgcagttga tgcgggcgca gtccatgccg gcggcgacga ggttgcggat cagggtcccg
     1921 ccatcttcgg cggcttctgt cggcagggtc accatgatac gcgtccccgt cttggccgta
     1981 ccaaaaatcc gccctgtgtt ataggccagg agcgtatcgc cgtagaagaa gcgcttcgtc
     2041 tggggataag gaatgagttt atccgccggt tcatggcaaa tccggccgag ggaggccatg
     2101 acggcatcca gattggtaat cgcccgggct tcgccgcgtc ccagggagga caggcccaat
     2161 ggcagaaggg agcgctggat tttccgcagg tcatggcagc gcagggctaa gtaaaaggcc
     2221 atattccagg cgctatagac gaagcgcgtc cgctgtatct gcggcagcca gtgctggaat
     2281 aaggcgtcgc cccgggaaag gacgtcgcgc cggagctggc agaggacgtc gtagagttcg
     2341 ttcgtcgatg tatatatttc ctgttccaat gtaatcacct caatatcatg aattttcttc
     2401 atatctatta ttatattccc gacatgtggc tttctcaatg ctttctgttt tgttttacaa
     2461 cgattttaca gatatatttt ttacatatgg ataatctatg atatatatat ttttctaatc
     2521 ataaaattca tgatatactg gcttttgttt ctacgaaagg agtcttacta tggaatggat
     2581 tacgacgacg gatattcaca tcctctttgg gctgcaccgc ctggccgggc cgcacatgga
     2641 tgctttcatc acttacttca cgacggtgat caatacgctt tggctctggg ccatcctggc
     2701 ggccgccggc ctttggaaac ggcggagccg ctgggtcacg cttaccatca tcgtcgccgt
     2761 catcctggcc atcctcgtcg gcgatgtggc catcaagcac ttcgtcatgc gtatccggcc
     2821 gtatctggtc atccccggtg cgccgacgct ggatacgctg aagtacccga tcagctactc
     2881 ttttccgtca ggccattcgt tcttcttctt tgccggggcc acggtcttat tccggtcctg
     2941 ccggcgcctg ggcatagcgg cctacggcct ggcctgtgtc gtcgcctttt cccgtatgta
     3001 cctgttcatg catttcccca gcgacgtcct gaccggtgcc gtcctgggca tcatcatcgg
     3061 aaatattgcc tggcagatat gcgggtatgc caaaaagagg ctgtcacatt aagac
//
LOCUS       DAWABZ010000178         2990 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_7330_length_2990_cov_2.577172, whole genome shotgun sequence.
ACCESSION   DAWABZ010000178 DAWABZ010000000
VERSION     DAWABZ010000178.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7330_length_2990_cov_2.577172"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    913..922
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          668 a          799 c          789 g          724 t
ORIGIN      
        1 acggtgtagg cccggcgggt gatcggttcg aacatggccc gcaggtcgag gtactgatgg
       61 gccgtgatgt gcattttatc ggtgccgacc tggccggaaa tggcgaccag cggggccccg
      121 tcgcagtcgg catcggcgac gccggtgatc aggttggtag cccccgggcc gagggtggcc
      181 atgcagacac cggctttgcc ggtcagacgg ccatagacat cggccatgaa ggctgcgccc
      241 tgttcatggc ggactgtaat gaattcaatc tttgaatgtt ccagggcatc catgacggcg
      301 aggttttctt cgccgggaat accaaaaata taatggacgc cttcttcttc cagacattcg
      361 acgagaaagt cagctgtgtt gcgcgtcttc ttttgttcca tggtgtgaac actcctttga
      421 cgatagatgg ataggttgaa attcgtgttg caacctgtat tataatatgg tatagcttgt
      481 ttttcaatca tttgagaggg aggattttat gaacgttcta atcagtgcct gcctgctcgg
      541 ccagtactgc cgctatgatg gtcagacgaa ggtttatccg caggtgcagg atttgctcgg
      601 gcgggatgat atccatttga ttcccgtctg tccggaacag gccggcggcc tggcgacacc
      661 gcgtccgccg gcagaacggc agggagaacg ggtcgtcacg gcagacggta ccgatgtgac
      721 ggcgcaatac cggcgtggtg ccgaagcggc cctgtacttg gcccgcctgt tccattgcac
      781 gaaggccatc ttgaaagaga aaagtccgtc ttgtggacat ggacagattt acgatggtac
      841 tttttcacgg accctgacgg ctggcgacgg cgtgacggcg gcgttattac gcgcagaagg
      901 ccttgaaatc atnnnnnnnn nnaaatcata ggggaaagcg acctgctctg acccttgctt
      961 ttatacccct ggtttgctac aatatacgag tacgcaagtg aggaggtttc agcgtgaata
     1021 cgtttctaca tgaagtttac gaatggattt attccatcat catcgcgttg gccattgcca
     1081 tggtcatcca tatcttcttc ttccagccga cacgcgtttc cggtgaatcg atgatgccga
     1141 ccctgcacaa cggggaatac ctcatcgtat ccaaatggaa tcacgtcctg ggggaagtcc
     1201 cgaactatgg agatattgtc atcatcgaca gccgcgtcca gtatccgcgc acgtggaaag
     1261 atgacgtcgc agaaccgatg aacaattaca tggctttctt taatcatgat ctgcagacca
     1321 agaacatctg ggtcaagcgg gtcatcggcc gtcctggcga cacgctggcg ttccatgatg
     1381 gcaaggtatg gcgcaacggc cagcctttag atgaaccgta catcaatgaa ccgatggaat
     1441 acagccgcaa aggggaaatc aagatccccg aaggctatgt cttctgcatg ggtgataacc
     1501 gcaaccacag cagtgacagc cgcttcatcg gcccggtccc catcgaccac gtcttaggcc
     1561 acgtcatctg gtaggcctca aaagagcata cgacagttcg caatccatcc tgtcggtaaa
     1621 caaaactagg aagcattaca atggtatctt gcaatgcaga cgggattgtt tcggcaaccc
     1681 cgtctttttt tcttttcttt ggaaggaggt tacgcctatg tataattttc tcgcgaatac
     1741 gaaacgcatg accgtcgctg ccatggtcat ggccctgtac atcgtcatct tgtacgtcac
     1801 tcaggggatt tcctttggcc cctatcagat ccgcatcgcc acgtcgctct atgcattggc
     1861 ctttgtatat cccttcctgg tggtcccctt ggggcttgcg aacttcattg ccaatttcct
     1921 cttcggcggc ctgggcttta tcgacatgat cggcggctgc ttcgtcggca tggccacgac
     1981 atggctcatc gtccagatcc gtcagcgtca gtatagtcca tggctggctg ccctgcccat
     2041 ctggtgggtg ccggccctct gcgtcagcgc ctggctgtcg tacctgctcg atttgcccta
     2101 cgccgtcctg gtcagcagcc tggctgtcgg tcagctgccg ccggccatct gcggggtcct
     2161 gctcatccag gccctgagcc atcgttatgt cttgaagaag gagttttgat atgagccgaa
     2221 gtaaagaaga actgcagggc gtgacccagc tgggcaatca acacacgacg tacgcccagg
     2281 actacgaccc gtccctgttg gaaacatttg tcaacaagca tccagaccgg gattatttcg
     2341 tcaagtttaa ctgtcccgaa ttcaccagtc tctgccccat gacgggccag ccggactatg
     2401 cgacgattta catttcctac gtccctggtg agcgcatggt agaaagtaaa tcgctgaaac
     2461 tctatctgtt cagcttccgc aatcacggtg atttccatga agactgcgtc aacatcatca
     2521 tggacgacct catccaggcc atggacccga aatatatcga agtatggggg aaattcctgc
     2581 cccgcggcgg catcagcatc gatccgtact gcaactacgg ccggcccggc acgccctggg
     2641 ctgacgtggc ctggcagcgc ctgtcccagc acgatttata tcctgaaaag gtagataatc
     2701 gctaaatcta ttacctttta cggctgtttc atgatataat aagtctaaaa acaacagaag
     2761 taaggggtgt tacctgtgaa attgaaaaaa atgcttttgg ccctgggatt ctccctggcc
     2821 ttgtcggcat cggtcttcgc agcggataat atgtggcaat ataaccagta tacgtccatc
     2881 gacatgaact cggccaagct ctattacgat atgggccgcc agatcctgtc ctttgagacg
     2941 gtggaacaat ccggcgaaac gactgactac tgcacctatg tgtataacat
//
LOCUS       DAWABZ010000179         2984 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_7346_length_2984_cov_3.856606, whole genome shotgun sequence.
ACCESSION   DAWABZ010000179 DAWABZ010000000
VERSION     DAWABZ010000179.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7346_length_2984_cov_3.856606"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          596 a          821 c          807 g          760 t
ORIGIN      
        1 tgacctgttt cggctaagac gtggttcaag gcgctggtca ggtcttccag ggtagtgagg
       61 acggtaccgt ccatatcaaa aattgcggct ttatatgttt tatgtttcat attcttctcc
      121 atggttactt tattttttgc cattttagcg caggctgctg gcataaatct tttccaggtt
      181 gtcgcgcatg cgatccaggt aggattcgtc atcttcactc gtttcgatgg tatggatcgt
      241 ttcgacgcgg gcaccgactt cacgggccag ggtttctgac gttttcgggc tgaccatgtc
      301 ttcgacgaag atggtccgca cgtcgttggc ccggcaatag tcggccaggc gggccagctg
      361 gcgggcactg ggttcgccgc tggaaaagac ggattcgacg ctgtgctggg tcaggccgaa
      421 gtcgcggcag aggtaggcaa aggcagcgtg gccggtgacg aaatccttgc ggggaacgct
      481 ggcgaatttg cgggcatatt cgtcttgcag ggcctggagc tgttttttgt aattcgccgc
      541 attgttgcgg taatagtcgg cgttggccgg gtcggcttca gccagggcgg cggcgatgtt
      601 agatacttcg atttgggcgt ttaccaggct cagccagatg tgagggtcgt aggcgccgtg
      661 ttctttgaca tcttcctcat cggtcagggc gatgggcgtg acgccttgcg aggcattgac
      721 cgtatggagg gatgtattct tggctgcttc gacgacctta ggggcccacg gttccatgcc
      781 caggccgctg taaacgaaga gctgagattt accgagatgt ttaatagtct gcgtcgtcgg
      841 ttggaattca tggggctctg tcccatcggg gatgagggtg taaacatcga ctttatcacc
      901 gcctacggct tgagtgaact ctttcagagc gtcgaaagtg acagcgactt ttaatttccc
      961 gtcatcgggg acatcggcgg cgggaccaca gccagccagg ccgatgatgc ataagcaggc
     1021 cgccaggacc atgccgtaac gggccaggcg gttgagggta tgtaacaaca ttgataagac
     1081 ctcctatggc gtccatcgta tcacgggcgc ctttttttgt caattatttg caaatgcctt
     1141 gcattttcgt tttcacgggg ctataataag ggctcgagac tacgacaagg gaggcaagct
     1201 atgattgatt tacagcacgt ttatttcgcc tatgaacacg gtcagtacct gttagaagat
     1261 gtcaacatga ccatccgcga cggcgactat atttccgtcg tcggtgaaaa cggcagcggc
     1321 aagagtacgc tcatcaaact catcctgggc ctgttgtcgc cgagccgggg gtccatttcc
     1381 aatacctatc gccatcgggc ctatgtgccc cagcgcttcg agacgctgaa cagccagttc
     1441 ccgattacgg tctgggaagt cctcaactgt taccgccagg tcctgcactt gaagcataaa
     1501 gaaatcatcc gggactgcct ggaacagatg aatgtctatt ccctgaagga ccagctcatc
     1561 ggcaacctgt ccggcggcca gtgccagaag gtcctcatcg cccgggccct catgggccat
     1621 ccggacctgc tcatcttcga tgaaccgtcg acgggaatcg atgtgcgcag tcaggaagag
     1681 ctctacccgt ttatcaagga tttaaatgaa aataaaggcg tcacggtcat tacggtcgaa
     1741 cacaatctga aattcgctgt ccgcaattcg acgaagatgt tccacgtcgt cggtggccgc
     1801 ggccatttct gctcgcccaa ggcatatctc gatgaatatg tatcagccac gatgggaggg
     1861 gataagtaat gtttgaatat gcctttatgc agaatgcttt tatcgtagcc ctgctcattt
     1921 ctatcgtctg cccgctcatc ggcatgttcc tggtcctgcg ccgctattcc atgattggcg
     1981 acgccttgtc ccatgcgtcc ctggccggcg tggctgtggg tctcctcttt gactggaacc
     2041 ccgttctcag cgcctttggc ctgaccagtt tcttcggcat cctcatcgaa gtcctgcgcc
     2101 gccgcttccg ccgttatgcc gagctcatcc tggtcatcat cttatctctc tccgtgggca
     2161 ttgccattac catcatcagt gccggcctgg tccacacgaa tgtcgaatcc ttcttgttcg
     2221 gcagtatcct caccgtgtcg acgggcgatg tctactccgt cgtcgccctc agcatcatct
     2281 ccctggccgt cattgccaga ctctatcccc agctggtcat gctgaccttt gatgaagacg
     2341 gagcgcagat tgccggtgtc cgcagcaagc tcatcaacta tatctttgcc gtcctggtag
     2401 cggcgacgat ttccgtttct atccgcatcg tcggcatcct ggtcatcagc tccctcatcg
     2461 ccctgcccgt ggcgacggcc ctgcagctgc gcaaaggctt ccggcggacc ctcatttact
     2521 ccgtcgtctt cagcttcatc gacattctgt cgggcctgct gctgtcgtac tacatcgatg
     2581 ctgcgcctgg cggcgtcacg gccttgacgt cagttgccat gctgcttctg gtcattgcct
     2641 ataaagaatg tgtcttttcc ctgcaagaaa agaggcgccg tccatgaatg acgattataa
     2701 agcctgttta cgcgaacatg gcctgaagag taccaaggcc cgcaacggcg tcctggatat
     2761 cctgagccgg gaaaccagtg tcgtgaccgt cgaaaccgtc tatgcagccc tgttggaaca
     2821 aggatgtaaa gtcaattttt ccacagtgta ccgcatcctg gaaatgttta cagagcgtaa
     2881 actgacagaa aagatattcc tccccgatga agggaaatac ggcttttccc tccgccgtcc
     2941 cggccataca cacaggctca tctgtttaca atgccataat gtcg
//
LOCUS       DAWABZ010000180         2948 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_7437_length_2948_cov_3.083650, whole genome shotgun sequence.
ACCESSION   DAWABZ010000180 DAWABZ010000000
VERSION     DAWABZ010000180.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7437_length_2948_cov_3.083650"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    281..325
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          585 a          791 c          829 g          698 t
ORIGIN      
        1 tacggacgga taccgccgtg aagggccagg ccattggtga tggcgcccat agccagttca
       61 cggacgccga agtggacgtt gcggccgctg taatcggtct tgctgaagaa gccttcgcct
      121 ttcatttccg tcttgttcga cggagccagg tcggccctgc cgccaaagag ctgaggcatg
      181 atgtctttca ggcggttgat catcgtgccg gacaagctgc gcgtagcctg cggtttatct
      241 tcataagccc agaaatcttc gtcattcaag agggcctgtg nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnncggct ttctggctca attcagcata atgagcatag
      361 acttcgtccg ggatgtagaa ggtttcctgc ggtttcggcc agcccaggtt ttctttcatg
      421 gcgacgatgt tttcttcacc cagcggcgag ccatgagcgg cagccgagcc ctgtttggcc
      481 gggcagccat agccgatctg ggtcttgatg gtaatgaacg acggatgttc cttgtcggct
      541 ttggcttctt cgatggcttt gccgatggca tcgaggtccg tgccgtcttc gacggtaatc
      601 gtctggaaac cgaaggcagc catgcgggcc tggacatttt ccgtaaaggc gatgcccgta
      661 ttgccttcga tagagatgtt gttgctatcg tagaggacga tgagtttgga aaggccgagt
      721 gtccctgcca gggagaaggc ttccgacgag atgccttcca tcaggcagcc gtcgccgcag
      781 agggcgaagg tgtagtggtc gacgacggga taacccggtt tattgaaaat agctgccaag
      841 tgagcttctg ccatggccat gccgacagcc atgcccaggc cggcgccgag ggggcctgtc
      901 gtcgcttcga cgcccgtcgt gtggccgtat tccggatgac ccggtgtcaa ggagccatct
      961 tgacggaact gtttaatgtc gtccatggtc aggccgtagc cgtagaaatg gagcagcgaa
     1021 tacaacagca tcgagccgtg gccagccgag aggataaagc ggtcgcggtt cgcccatttc
     1081 gggtcctttg cgttatggtt catgtgattt gcccagagtt cgtatgcaat gggggctgcg
     1141 ccgaggggca ggccgggatg gccggagttg gccttctgga tggcgtcagc cgacaataca
     1201 cgaatggcat ttacacaagt cgtatcaatg gtgctcatta gtcaaatcgc tcctttttat
     1261 gtgatcgaaa ataaatagac attcacttta ttatgtaaaa aaaggacccc ttctgtcaat
     1321 ggcaaaaggg gattttggga gaaatttata tatgctataa taaagaagtt tcttaatttt
     1381 ccgagaaagg atagactcat gacgaaaaaa tactttttct ttgatatcga cgggaccctg
     1441 ggcctgggga tctccagcat catcccggca gacacgctgt actgcctgcg ccgcctcgtc
     1501 cagaaaggcc atttcatcgc catcgcatcg ggccgcctgc agcacgatac acagaccttt
     1561 gccgaccgct acggcatacc ggccgtcgtc gccgatggcg gcaacagcct gtccctggac
     1621 gggaaaatcc tgaaaatgac cggcctgccc ctggagaact gcaaagccct gctgcgcgac
     1681 ctgacggacc gccactatcc ctgggccgtc gtcacggaca ataccatcaa ccgctataca
     1741 ccgtatgctg atttccccca cgacgacccg cgcaattaca tggaaaccgt catccgcccg
     1801 gtccccatcg aaaatctgac ggctgtctat aaaatcatgt acgcccggcc ccagcgcctc
     1861 gaagacgaac cgccgacata cggcctgccc cacctggaat acatcgacca cacgtacctc
     1921 atcgaaccga ccgacaagag ccgcggcatc ctgcgtaggc tggacctcgt cggcggccgc
     1981 ccggaagatg ctgtcgtctt cggcgacggc atgaacgaca tttccatgtt ccgcaagccc
     2041 ttcttctcca tcgccatggg caacggccgg gatgagctga aagcccatgc cgactacgtc
     2101 acgggcgaca acgataaggg cggcatcctg gaagcctgca aaaaattcgg ctggctctaa
     2161 gggatagtcc gtagtttgta gtctgtagtt tgcagtttct agttgtggcc ggcgcctttt
     2221 aacagggctt cccgcaggcg gtcttcgatc tggaccagtt cggcttcggc ggcctgccgc
     2281 ttctgccgtc cttcctgctg gatgcgcagc gtatcttcga tggtgctgat caaatcttca
     2341 ttgacctgct tgaccgtttc gatatcgacg atggcccgtt cattttcgac ggccgtatcg
     2401 tgggaattct gcttgagcat ggcggcgttg cgcttgagca gttcattggt cgtattagac
     2461 acagcctgct gcatttccag ggcttctttc tgccgcccca gccccagggc caggaccatc
     2521 tggttcttcc acaaggggat ggtgttgtaa atggcactct ggatgcggtc gacgaggatc
     2581 ttgtcgttgt tctggatgat gcggatctgc ggtgccgtct ggatggcaat ggtcttgctg
     2641 attttcaggt cgtggacctt cttttcaaag cggctgacgg aattcttgaa atcctggacg
     2701 acctggacgg ccatgggatt atctgaaacc tgagcctggg cttccagttt gggaatcgtc
     2761 gtcaagttca gttcctgtat cttttcttcg ccagcctgga tgtagagctg gagctgtttg
     2821 aagtattcca ggttcttatc gtagaggccg tcgaacatgg caatgtcctt catgagggtc
     2881 attttctggc tgtccaggcc ggcctgtatc ttgtcgatct gggcggccag cgtcgtatag
     2941 gactgggt
//
LOCUS       DAWABZ010000181         2920 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_7507_length_2920_cov_3.603141, whole genome shotgun sequence.
ACCESSION   DAWABZ010000181 DAWABZ010000000
VERSION     DAWABZ010000181.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7507_length_2920_cov_3.603141"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          726 a          796 c          813 g          585 t
ORIGIN      
        1 gaacctcgca cgttggcgtt cattcagttc attgttcagt tttcaaaggt catcgccatc
       61 accgcttgtc gcggcgactt gcttatttta acaaactcag aagagcttgt caaggatgtt
      121 ttttgaactt tttcaaagct caatcccatc ctcaagtgcc aaggccttcg ccctaacagc
      181 ttgtttagta tatcacatta tgtcgttttg tcaagaacta aattttaaat cctataaaac
      241 ttaaaacttc atttccgagc atttccgaac tcatgcgaca taaagtattt tatcattgaa
      301 aaaaaaagct gtcaagcaaa ttttgacagc tttttacatt tttttcgaga ttaccttact
      361 tggctttgac cagtccttcg ctgatgcttt ccaagtcctt ctgcttgctt tcgacgccga
      421 ggctcaagac gatgacggca acggctacga agaccaaggc gaacatgtag aagacactgc
      481 cgaaaccgaa gttccgggcc aacagggctc cgaccatcat gggcgcagcc atgccgccaa
      541 agcggccgaa gccggcagcc cagccgcttc ccaaggctcg gatagccgtc ggatactgtt
      601 ccggcgtata cgtatagagg acgccccagg caccgaggtt gaagaaagac atgacggagc
      661 cccacatcat gagggtcgct gccgtcgacg catggccgaa gaagtagctg gcgacgccgc
      721 tgaacaagag gaacgtcgac agggtatact tgcggccgat cttatcgacg agccaggccg
      781 cgcagtaata gcccggcagc tgggccaggg tcatgatgag gacgtattca aaggtcttga
      841 cgacggtaaa gccctgctgg aagaccagcg aaggcagcca catgaagatg ccgtagtagc
      901 tgaagttgat gccgaaccag accagccaga gcatgatcgt ccgcgacgca aagggctttt
      961 tccacaattc ccggaacgaa gccttggcga cgaccggcac ggtttccttt tcgctgacga
     1021 agggcgctac gggtacgtgc agctgccgct ccagggagac gacgatctgc cgggcttcgc
     1081 cgacgcggcc atgagccagc aggtagcgga ccgattcggg catatgcagg cggatcaggc
     1141 agacatagag tgccggcagg gcgccgatga ggaaggccat gcgccagcca taaaggggaa
     1201 tgaagaaata ggcgatgcag gctgctgcaa tccagccgag gccccagaaa ctttccaaga
     1261 ggacaatgaa gcggccccgt acgcgggacg gcgcatattc ggaaacgagc gtcgccgcta
     1321 cgggcagttc tccacccagg ccgaagccga cgaggaagcg gaagacgagc agggattgat
     1381 aattccagga caaggcgcag aaggccgaag caatgctgta caataagacc gtaatggtga
     1441 agaccttctt gcggcctatg cggtcggcga tggtgccgga cacgacggcg cccagggcca
     1501 tgccgatgag gccgatgctg ccgatgaggc ccatctgccc cggtgccagg ccccattctt
     1561 tggccagtac aggcaggata aaggcgatga gccctgtatc catcgagtcg aagagccagc
     1621 caaggcccgt gaccagcagc agtttatagt ggaaacggcc caggggcagc gcttccagtc
     1681 gttctaatac cataacaaca gttcctccta tctgtacata tgtcatgaca gttatattta
     1741 catggtctta ttgtaccgat atagagggga aatgtcaatc cttatataag acgccgacgt
     1801 cgcgcagggc ctggccgatg gcatcgagtt cttcttccgt cgccgcttcg acgagatgcg
     1861 tatggatgcc gccgctgatg gtactgagca tttccgcatg ggaatcatgc atgcgcttga
     1921 tatactgctg gatatcccgg cgggaatgaa ggttcaaggt cccttccagg ttgccataga
     1981 cgtcgtgttc gatgacgacg ttgtggacga tgccgccatt atcgacgatg gcattgagct
     2041 ctgcttccat caaatcgctg ccatggcagc agacgaagac ccgcgtacag cccggtgcgg
     2101 ccggtgtgac cagctgatag ccccggggcg tcgagatgat ggcaatcccc tgggcgcgca
     2161 aaatagccac gtcgccgacg atgatctgcc ggctgacgtt gcaggctttc gataaggccg
     2221 tcccggtgac ggccgacgac gattcttgaa gaatctgaag aatcttttct ctccgtgcac
     2281 tactatccat aaacaaagtc cctcctaaat atctttatag acctgctgtc cccgcgtggg
     2341 aatatcgaac agcatggcca atccccgtcc ccacagccag cccatgccga tgcccggcgc
     2401 aaagcccagg acctcgttga acagccacga atgggtgatg aagggcatgt ggtaggccac
     2461 ggcctggaac gaggcgaaaa tcaggcagaa aaagagattc accggcaggg ccgtccgcaa
     2521 gggcggacaa cgaaggctgt gctgcttgcg gcaataggtc agttccggca ggcccttctt
     2581 gaaccacagg cccagaccca ggcccaggaa aaaggccatg aggaaacaga gggtcgtcaa
     2641 aaaaggatat tccgtataat aatataatac agccgctgcc cagatggcaa ccaggacgca
     2701 ggggcctgcg aaccaccaca ggcggcacat gcggctcttc cggaagtaaa tgccataggc
     2761 gatcatggcc atagccatgc agagttgtat ccccgtcagg atatcatagg cgctcatcac
     2821 ggtcggctcc ccctttctgt tcctctttga acgatgagat gatgtatgtt cagtatatag
     2881 ggtaaagagt gaacgtactc ccgcctatag aggcgggagc
//
LOCUS       DAWABZ010000182         2878 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_7605_length_2878_cov_3.159405, whole genome shotgun sequence.
ACCESSION   DAWABZ010000182 DAWABZ010000000
VERSION     DAWABZ010000182.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7605_length_2878_cov_3.159405"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          554 a          802 c          879 g          643 t
ORIGIN      
        1 gtcatagacc tggctcaagt cgcggttatc tttcttcatc tttttataaa tgctgtaaaa
       61 atgcttgggc cggccgttga tttcgcagtg gatgtgggct tcgtcgaggg ccttcttgag
      121 gacgtcgatg gcttcgttga cgatttcttc gcggacgtgg cgcttctgct tcatctggtc
      181 gacgaggtcg tagtacttgt ccggttcgag gtagcggaag gacaaatctt ccagttccca
      241 cttgatattg aaaatcccta agcggtgtgc caggggggca aagatttcca gcgtttcctg
      301 ggcaatacgc ttctgcttgt cgctgcgcat gtagcgcagg gtccgcatgt tatgcaggcg
      361 gtcggcgagc ttgatgacga cgacgcgcac gtccttggcc atggccagga acatcttgcg
      421 catgctgttg agctgctggt cttctttggt acggtaattg aggcggctca acttggtgac
      481 gccgtcgacg aggaaggcta cttccttgcc gaacatcttc ttgatatctt ccagggtgta
      541 cgacgtatct tcgacgacat cgtggaggag cgacgccgta atggtcgtcg tatctatttt
      601 catatctgcc agtatctggg cgacggccag gggatgcagg atatacggtt ccccgctgac
      661 gcggcgctgg tccttgtggg cctcttcggc caggttatag gctttggtca cgtcatcgca
      721 tttggcctgg ggcagatacg tatgtatcgt gtcgatgagg tgtttgaatt cgacatcttt
      781 atctttaaat tccataatat gaaacctcct cactccttgc agtacaattc atacgtcggc
      841 gacgcatgaa gatccatttt ccccgcaagg ataggaaaat aataagctgg gccgtcgtca
      901 tcatggcgta ttttgcggac gccgatttcc cggaggacga cgatggcagc gtaaatggtg
      961 tggacatcat aacagtcccc ctggtccgca gccagacggc ggcggacctg ccagaccggc
     1021 atgccccagt cggggatgca tttcttgagg gccatgtaaa tatcgaccat gaccgcccgg
     1081 ttgaggacga tgtggcggcc tggcaggtgg acgtcctgga tgaccagctg cggcgacgac
     1141 atgccattga agtcgttccg ttcgagctgg aaggccacgt cgatgacgtc gccttcgacg
     1201 atggcgtcgc acaggcccgc ctgggaccag gcgacaccgg aaaggcgtgt cctatcggcg
     1261 ccgcgggcga cgaggcggac gtgctgcttt tcccggccga tgggacggat ttcctctacc
     1321 gtagccccgc tcagggaaaa gacgggccgg ctgttgccca tgccataggg ctccaggcgg
     1381 gacagctcag caatgaggtc caaggtcaca tcggccggtt ccaattgctt gtcgatgtgg
     1441 accaagggga cgtagtcggc ggccgtcata tgcgctgccg catagtccag caggcgccgg
     1501 cgcagggctt cgatgttctc ggccctgacg gaaaagcccg ccgccatggt gtggccgccg
     1561 aactggatga gcaaatcctg ggcgtattgc agggcttcgt acatgttgaa accgctgata
     1621 ctgcggcacg atcccttgcc gatgccgtcg cggacggtga tgaccaggga cggccggtaa
     1681 tacgtctcta ccaggcgcga tgcggcgatg ccgatgacgc cgacgtgcca gccctcatga
     1741 taggcgatga ggacgccgtc gcggcccctc gcctgccctt cgatctgggt gatggcttcc
     1801 tgggcaatgg tgtgctcaat gtcctggcgc tccgtgttga ggcggctcag ttcggctgca
     1861 tcggccgccg ccttggccgc atcggtttcc aggagcagtt cgacaccctt cgtcgcatgg
     1921 ctgatgcgcc cggccgcatt gaggcgcggc gccgccgtaa aggcgatacg gccggccgtg
     1981 atatccttcc cgctgagatt ggacgcatcg aggagggcct tgatgcccgt ccgcttccct
     2041 tctttcatgg ccgccaagcc atggcggacg aggatacggt tctcgccagt cagggggacc
     2101 aggtcggcaa tggtccccaa ggccgccagg tccgtatagc ccggcaggtc ttcatggcag
     2161 tgatgctgcc acagggcccg gcagaggacg taggccacgc cggcgccggc cagctcctta
     2221 aagggatagg ggcagccggc ctgcttggga ttgaggacgg ccagggctgg cgggatctgt
     2281 tccggcggtt cgtgatgatc cgtaatgatc atatccagga ccggcgagaa ctgctggacc
     2341 aggtcgtgtg acgaaatgcc gcagtcgacg gtgatgagca ggtcggcctg ccggctcagc
     2401 tgctccaggg ccccttcgtt gaggccatag ccttcgctct cccgttcggg aatgtagaac
     2461 tgcggcgatg cgccgaggtc gcgaaggacg gaataaacaa gagacgtcga cgtaatgccg
     2521 tcgacgtcat agtcaccgta gatgacgatg cgcttttcag ttatcgcctg ctccagcctg
     2581 atgacggcat tctccatgcc tttcatcagg aaaggatcgg ccatatcggc caaagtatcg
     2641 tgaagaaaat ggtcccctgc ccggacatcg gcgatgccgc gattggccag tacctgtgcc
     2701 aagaccgggg atatatgtaa ttgtgcggac aaggcctcca tgagcccctc gtcgggactg
     2761 ccgaactgcc atcgtttctc catagctatc acgacacatt ctcaagaata atcactatca
     2821 ataaatcaac tattatttta tcactttcta ttctcaaaat aaaggctaaa tctatagc
//
LOCUS       DAWABZ010000183         2872 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_7625_length_2872_cov_3.808307, whole genome shotgun sequence.
ACCESSION   DAWABZ010000183 DAWABZ010000000
VERSION     DAWABZ010000183.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7625_length_2872_cov_3.808307"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          630 a          835 c          744 g          663 t
ORIGIN      
        1 ccgcggctac ggtgccagcg tcgtcctcta cggggacttc ttcgacgaag ccgctgccaa
       61 agccgttgaa ttgacgacgg aacacggcta caccttcgtc catcccttca atgacccgga
      121 agtcatcgcc ggccagggga cgattgccct ggaaatcatc gaacagctgc ctgaagtcga
      181 tgccatcgtt gcaccgattg gcggcggcgg cctcatttcc ggcctggccg tagcagccaa
      241 gagcgtcaac ccgaagatta aagtcatcgg cgtccagacg gaaaacatgc cgtccatgaa
      301 ggaatccgtc gaaaaggggc aggtcatcac ctacaacggc aaggctaccc tggccgatgg
      361 catcgccgtc aagacaccgg gcgacctgac ttttgatatc tgcaagaaat acgtcgatga
      421 aatcgttact gtcgatgaat cggaaatcgc cagcgccatc ctgctcctcc tcgaacgggg
      481 caagaccgtt gccgaagggg ccggtgccgt accggtagca gccctcatga gcggcaagat
      541 ttccggcatc cgcaacaaga aagtcgcagc cctcgtcagc ggcggcaaca tcgacgtcaa
      601 taatatgacc cgcgtcatca accagggcct gatcaagtcc cagcgcaaga ttttcttcca
      661 gaccgttatc cccgacgtac cgggcgaact ggtcaagctc ctgaccctca ttgccggtac
      721 caatgccaac gtcctttcca tcacccatga acgcagccag cacggcatcg acatgggcat
      781 gaccgctgtt tcactggaac tggaaacggc caacgaaaag cacgttgaaa aattgatgac
      841 catgctcaaa gaccatcatt attttgttac cttgaagtag tggaggcctg atcatggaac
      901 agcagaaaat ggaacgcggc ctgaagccgc gtcacgtaga gatgatcgct cttgggggga
      961 cgattggtgt cggcttgttc atggggtcgg ccaacaccat tcagatggcg ggaccgtcgg
     1021 tattgctctg ttatgccgta acgggcgtcg tcatgttttt catcatgcgc atcatggggg
     1081 aaatgcttta tcaggaaccg gttacgggct cctttgccac gtatggtcat aagtacatca
     1141 gtcctttcgt cggctacctc actgcctgct cctattggtt cttgtgggtc gtcgtcggcc
     1201 tgtcggaaat cacagccgtc ggcatctatg tccactactg gttcccattg ctgccgcaat
     1261 ggatatcggc cctggccggc atggccatcg tcgctgcggc gaatatggcg gccgttaaat
     1321 attacggcga atttgaattc tggttcgccc tgatcaaagt cgtcaccatc atcgtcatgc
     1381 tcatcgtcgg tgcggcggtc atcctctttg gcttcggcaa cggcggcgtc cccgtcggca
     1441 tcagcaacct ctgggtcaac ggcggcttca tgcctaacgg ctggggcggt atgctggcgg
     1501 ccatgtgcgt cgtcgccgcg tccttccagg gcgtcgaact catcggcatc acggccggcg
     1561 aagcccagga cccgaaacat actttgaaga aggccgtcaa caatatcgtc tggcgtatcc
     1621 tcatcttcta catcggctcg attttcatca tcctctgtat ctatccctgg aatgaagtca
     1681 gctatatcgg cagtcccttc gtcatgacct ttgccaaggt cggcatctcg gcagcggcag
     1741 gcatcatcaa cttcgtcgtc ctcacggcgg ccctgtccgg ctgcaacagc ggcatgtaca
     1801 gctcaggtcg tatgctctat acgctggccc agaacggcca ggcgccgaag attttcgcca
     1861 aattgtcacc gaccggcgtg ccgcgcaaca gtattgccgt caccatcgtt gtcctggcta
     1921 tcggcgtcat cctcaactat ctcattccgg actccaaatt gttcctctat ttatacagtg
     1981 cctccgtttt tccgggcatg gtcgcctggt tcgtcctggc ctatgcccag aaaaatttcc
     2041 gcaaagcctg gggaccggaa gtcatggcga aacatccttt caaatcgccg ctctatcctt
     2101 attccaatta cttctgcatc attttcctga ttctcgtcac catcggcatg atttacaatc
     2161 ctgatacgcg catgtccatc ttctcgtcgt gggtctacat cgccgccgtc gtcatcgcct
     2221 ggttcgcctg tggcctgaac aagaaacagt acgataaata agggcacctc atcgaagggg
     2281 ctggaggaga tgcggcgacg gtcgccaaaa ctgaatcgga atcgtgatta tctgtcgcag
     2341 gtctatggct ttccctgggc ctgcgatgta taatacagtt agattaaaat taaggagatg
     2401 catactcatg gcagttaatg ttatcagcac gactaaagct ccgggcgcag taggcccgta
     2461 ttcccaggct attaaagccg gcgatttcct cttcgcatcc ggtcagattg ccatcaaccc
     2521 ggaaaaaggc aagatcgttt ccggcggtgt cgtcgaacag gctgaacagt gcatgaagaa
     2581 cgtcggcgct atcctcgaag aagccggcct ctcttacgat gatgtcgtca agacgacggt
     2641 ttacctgacg gacatcaact tcttcggcgc cgtcaacgaa gtctatggca aatacttcca
     2701 gaagaacctg ccggcccgtt cgtgcgtcga aatcagccgc ctcccgaaag acgtcttcgt
     2761 agaagtcgaa gtcatcgcct attgcgggaa ataagctgga tttatagtgg ctcgtgattc
     2821 gtggctcgtt attcgtaggg cggcccgctg tgccttaaat ttaaaactat cc
//
LOCUS       DAWABZ010000184         2854 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_7669_length_2854_cov_3.367631, whole genome shotgun sequence.
ACCESSION   DAWABZ010000184 DAWABZ010000000
VERSION     DAWABZ010000184.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7669_length_2854_cov_3.367631"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          632 a          908 c          761 g          553 t
ORIGIN      
        1 tcggccaccg ttgcgtcggc gccaagatca acggcaagat cgtccctttg gactataaat
       61 tgaagaacgg cgacatcgtc gaaatcatta cgtccaaagt cggcaagccc agcctggact
      121 ggctgaacat cgtcggcagc tcggaaagcc gctcgaagat acgcagctgg ttcaagaagg
      181 aaaaccgcga agaaaacatc gccaagggcc tggatgcgct ggaacgggaa tgtaagcgcc
      241 tgggccacga ctggaaagcc ctcaacgtcg gcggccgcct gggccgcgtg gccaagcaga
      301 tgaacgccgg ctccgaagac gacctggtcg ccgctgtcgg ctacggcggc tttgccgtca
      361 atacggtcct catcaagctc ctggaactgc acaagaaaga cttgcagaag caggaagaaa
      421 agaccaacag cctggctgcc ctggaaaaac tcaagaccaa gaaacccgtc aagcacaacg
      481 gcacgggcat cctggtcaaa ggcgaaccgg gcctgctggt ccgcctggcc aaatgctgca
      541 gccctgtacc gggcgatccc atcatcggct tcatcacccg cggccgcggc gtctcggtcc
      601 accgcgcgga ctgcccgaac gtcacgcacg gccagaacga cgtagaccgc ctcatcgacg
      661 tcgaatggga ttacgacggc aacgaacgct ttgaagtgaa catggaaatc gtcgcctacg
      721 accgcacggg catcatggcc gaaatcatgg ccaccctggc ggaaatgaag atttccatcc
      781 tcagcgtcaa cgccaagacc agcgatacca agaacgtcac catccacatg ggcatcagca
      841 tcaaggacct ggcccagttt gaattcgtgg ccaccaagat acgccgcctg aaggatgtct
      901 acgccgtcga acgctatacg ggagggagcc agacatgaga gctgtcgtac aaaagaccct
      961 gacgtcttct gtgacctcgg acggcgtcct caccggccag gcaggcaacg gcctgacggt
     1021 cctcctcggc gtttccgtca aggatacgga agccgacgtc cgctacctgg ccgacaagat
     1081 cgtccacttg cgtatctttg aagatgacca gggcaaactc aatcactccc tcctcgacga
     1141 gggaggggag atgctggtca tttcccagtt caccctgtac ggcgatgccc gccacggccg
     1201 ccgtcccagc tatatcgaag cggccaagcc cgaacaggcc gaagcattat atgagcagtt
     1261 cgtcgccgct gtccgcgcct tcggcgtcac cgtggcgaca ggccatttcc ggacccatat
     1321 ggtcgtatcc ctggcaaacg acgggcctgt gaccatcctc ttggacagcc agaaaacctt
     1381 ttaaggggga agtatactta tgagcatgaa atatatcagt atggaactcg gtcctatcat
     1441 gaccaattgt tatatcgtct acgacacgga tacgaaagaa gccatggtcg tcgatccggc
     1501 ctgggattat cccgccatcg acaaggctct gaccagccat ggcctgacgc tgaagttcat
     1561 cttcctgacc catggccatg ctgaccacat cggcgccctg caggaactgc gccagcgcaa
     1621 gaacgtaccg gtctacgtcg gcgccggcga tgccgacctc atttccaatt cgcacaataa
     1681 cctgtccctg ttcatgggcc aggccatcga atgtacgtcg gcagaccacc tggtcaagga
     1741 cggcgatgaa ctgacattgg ggcatctgca cttcacagtc ctggaaacgc cgggccatac
     1801 gccgggcggc ctcagcctgt acggtgaagg cgtcgtcttt tccggcgaca cgctgttccg
     1861 ctattccgtc ggccgtaccg acctctacgg cggctcgtcg aagacgctgc tccacagcct
     1921 ggaaacgaaa ctcatgaccc tgcctgacaa tacgctggtc ctgcctggcc acggcccggc
     1981 tacgtccatc ggcgacgaac ggcgtggcaa tccattcctg gacggaggct ggtaaaccat
     2041 ggttgcctat aagggcgcgg agttctggat gcgcgtcacc ctggccatcc tggccggtgc
     2101 gctgttcctc acggtccatg ccgtctactg gcccgtcgtc atttccctga tcctgacgtt
     2161 catcctcatg cctatccgcg atggcgtcat caaagggctg cggcgcctga ccggccgtca
     2221 tgtacccatc gaccttgcca tcctcatctc ctttgtcatc ctcatcgtca tcgtgacgat
     2281 tattaccaat atcatcgtca agccgctggt catccaggtc aatctgctgg cagccaactt
     2341 caacgatttg gtcagccaga cggcggccct ggtgacgcag cttgaaaatg agcagaccca
     2401 gttctatatc ccggaccagg tcaagaaaat cgtcaacgat gccttcgtca agatcggcaa
     2461 ttacggcatc gagggcatca ccaacctcat ccagtccgtc tttgccattg ccgggacggt
     2521 cgtcgaattc ttcgtcgtac cgatcattac tttctacttc atgaaagacg gcggccacat
     2581 ggtcaagagc ttcgtcgata tctttcccga aagctatcgc agccatctgg ccggcctctt
     2641 ccaggaaata cagcacgtcc tgagccgcta catccgcggc cagatcctca tgagctgtat
     2701 cattgccacc ctgactttcc tcggcatgtg ggccatgggc gtaccgtacc ccatggtcat
     2761 cggcatcttg gcggccatta ccgaatggat acccatcgtc gggcctatcg tcggcgccgt
     2821 gccggctatc ctgctcagcg cgacggtcag tttg
//
LOCUS       DAWABZ010000185         2850 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_7677_length_2850_cov_2.709839, whole genome shotgun sequence.
ACCESSION   DAWABZ010000185 DAWABZ010000000
VERSION     DAWABZ010000185.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7677_length_2850_cov_2.709839"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          625 a          769 c          866 g          590 t
ORIGIN      
        1 agacgggatg gaccaggtag gtgggatacg agtagtcacc caggagggag aagaccttgt
       61 ggacccattg cgggacaggc cggcattccc accagtagag gaggaagagc atcgtcgaca
      121 gggtataggc catgccgata gggctgagct gatggatagt gaagactgcc gacaaggggt
      181 cgtagcccag gtagtgcatg acgccgtagt aggcggccag catacccgct gtcgtcgctg
      241 cctggaagac gttgaccagc gtgccgtggc gtttcagcca gtccgtcacg gctgtgaagt
      301 gttctgccgt aaaggcaccg aacatgaaga tgaacaggta gtgcaggacg acgtagttca
      361 ggcggaagtt gaaggcgtcc tggatgaggg gattgtcgga atggaccgtc cagatgtagc
      421 tggaatagaa gttgaagacc atgttggcga aaaagaggat agtaaaagag agccacggcg
      481 ccttgtccat gaagcgcagg agcggccgcc acaacggcat gaggaagtag aaccacaaga
      541 ggatgaccag gaagtaaatg tgatacatgg ccaggccgta ccataaggtg cggaaaaagg
      601 cgtaaggctg gaagacactg aagttgtggc tgatgaccga tacgtagacc atgtagaaca
      661 gggaccaggc caggtagggg acgaagaccg tccgcaggcg gcgccgcagg tagtctttgt
      721 agacgaaggg ctggtccagg ggctggctgt agaacatgcc gaaggccgac aggaagaaaa
      781 aggccggtac ggaaaagcgg gacaggattt ccaggatggc caccagtccc agattaggcg
      841 acgggttgac gatagcgacg gatccgacgt ggatggcgat gacgccgagc atgcagagac
      901 cgcgcatata attgatggaa tgaatgaatc gttttgacat ctgacacctc gaaagataca
      961 ataaagataa ttgggataat gacatatcct attataacaa atttttccag cttggacagg
     1021 ggaggtttta tgacagaaga tataaaaaat gtgacccgta ccgggctcat cctggccgtg
     1081 accttgatcc tgcagggcct gcgcctggtc atccccatac cgccgcaggt cagtatgttc
     1141 gtcgtcggct ccctggtcaa tgcctgcctc atcgtggccg tcctcaccgt cggccgccgg
     1201 gccggcctgg tcgtcgccgc ctgtacgccc gtctttgcct ggctcgaagg gatgctgccc
     1261 ttttttccct ttgtctttcc cgtagccttg ggcaacagtt tctacgtctg ggcggcctgg
     1321 cgctggcagc ggtacggcct gccggctttc tgcggcggcg ccctgggcaa ggcggccatc
     1381 ctttacggct ctttttacct gctctttgcc tgcatcgcct ttccgccggc tgtgcggcat
     1441 atgctcttgt ttgccatgag ctggcctcag gtcgtgacgg gcgtcatcgg ggctgtcatc
     1501 ggcagggaag tgagccgctt cgccgcttct cgtcctgaat agaaaggaag gtattatgtt
     1561 actgcagatc ttttattatc tcccggccat cctcggcgtg ctgttcatcg ccgtcgggac
     1621 ctatcacggc tggaaattcc gcggcatgac gcggcgctgc aaggtcgccg ctgtagggac
     1681 gctcctcggg tttgaagaaa agaagatgaa gagcgggacc ttgtattatc ccgtcgtccg
     1741 tttccagacg aaagacggcc agacctatca ggcccgttac gcctttggcg atgccgaatg
     1801 ggactatacg gccggtgacg aactggattt gcgctataac ccggcggatc cccaggaaat
     1861 ctatctcgat cacgtacaga gtatctggca gcagtatgcc agtccctttt tcatcatcgt
     1921 cggcggcctt atctttatcg ctgcgtatta cttcatttta taaataaagg aggagctagt
     1981 tacgttggaa gaaaatgaaa gagccgtggc tgcgatgcgg cagtttttag aggccctggg
     2041 actggacctt caggccctgg ggatggaaaa gacgccgcac cgcgtggccc aggcttttgc
     2101 tgattttttc tcaggcctca aggaaaatcc cgatgacgag tggcaggatc ccattgcgac
     2161 tcagaccgat ggcctggtcg tcgtccgcaa catccgcttc cactccatgt gcgaacacca
     2221 cctgctgccc tttttcggct ccgtccatgt ggcgtattac ccgaaaaagg ggaccattgc
     2281 cggcttcggc catttcgtcc gcgccgtcga tatcctggct cggcggcctc agctccagga
     2341 acggatgact gacgaactgt gtcagtccat ccgccgcggc ctcgacccgg aaggcgtgct
     2401 gatcgtcgtc caggcgaccc atctgtgcct gaccatgcgc gaccgcctgg ccatcgactc
     2461 gaatatcgtg acgacggcgt cgtcaggctg cctgaaagaa gggaccgccg cctatgacca
     2521 ggcctggaaa ctgctcatgg aggaggaaaa agatggataa aatcgtcctg acgggacttc
     2581 atttctatgg ctatcatggc tgtctgcctg aagaaaaacg ccatggccag ccctttcaca
     2641 tcgacctggt actggaaacg gacctggaac gggccggcca gaccgacgac ttgacgcaga
     2701 ccgtcgacta cagcgccgtc tatggcgtcg tcaaggccgt cgtcgaaggg ccgtcgtatg
     2761 acctcatcga acgggtgggc caggtcattg ccgaccgcat cctggcccag ttcccgctgg
     2821 tccaggccct gaccgtgacc gtccacaagc
//
LOCUS       DAWABZ010000186         2810 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_7759_length_2810_cov_2.770599, whole genome shotgun sequence.
ACCESSION   DAWABZ010000186 DAWABZ010000000
VERSION     DAWABZ010000186.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7759_length_2810_cov_2.770599"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2552..2561
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          732 a          718 c          863 g          487 t
ORIGIN      
        1 cccctataat ggggaggctt ggcgggcttg ccacgtgcaa gtccctacag gacggagctt
       61 acgaactacg aattgctgct ttctttctgc aagaggctgt gtttgcgtcc gtaaaccatg
      121 tagatgatga ggcccaggat ggtccagcct gtgaacagca tcagcgtatg cggcgacagt
      181 tcggagaaga tgaaagcgca gcatatgaag cccagcggag cgatgacgtg tacagccggg
      241 cagcggaatc cacgcggatg gtccggatag agccgacgca ggacgaggat gccgacgcag
      301 gagcagagga aggcgaaaat cgtaccggca ctgcatgttt cagcgacaat gtgaatcggt
      361 gtgaaaccgg tgatgcaggc gacgatgacg ccgatgacga tctggatgat atacggagta
      421 tggaacttcg gatgaacctt gtaaaggctc atgggcatga ggccgtcacg gctcatcgag
      481 tagagagccc ggctctgagc gaagagcatg cccaggacga ccgtcgacag gccgcagata
      541 gcgcccgtac cgacgatggc cgaaccgaga tggaggccga tgtattccag gacgaaggac
      601 gccggtgctg ccgtatcgag catgggatac gggacgacgc cggtcatggc cgcacagacg
      661 gcgatgtata ggaccgtaca gatggccagg gccgaaatga tgccgatagg catgtcgtgt
      721 ttcggattct tcgtttcttc ggctgccgtc gccagggcat cgaagccaag gtaagcgaag
      781 aagagaacgg ccgtcccggc aaagacgccg tggatgccat aaggcaggaa cggctgccag
      841 ttcatcggtt cgatatgcgg tgctgccagg aagaggaaga ggaaaatcgt cccgatcttg
      901 acgtacacca ggatgcggtt gacgtgagcg ctttcgcgga cgccgatgac gaggagcgcc
      961 gtcgccagga cgacgatgag gaccgccggc agattgatga gtccgccgtc agccggcacg
     1021 gccgtcaagg ctttcggcaa gtcgatgccg caggaatgaa ggataccgac gaagtaggcc
     1081 gaccagccgc cagctacggc gctggcgccg accgagtatt ccaggatgag gctccagccg
     1141 acccaccagc caaagatttc accgagcgac gcataggcgt aggtgtacga actgccggat
     1201 acgggcagga acgaagccag ttccgaataa gcaagacaga cgaacgcgca ggtaatagcg
     1261 gcgaagacga aggataacat caggccggga ccggcgtatt tcgctgcgcc gataccggtg
     1321 aggacgaaaa tccccgtccc gatgatgata cccaggccca tgagggtaat atcgaaggcg
     1381 ccgagagcct tgttcaattt cttttcctgc gtatgggcca acaagtcatc gaaggatttg
     1441 cggcgtaaaa tattcataaa catgccccca atccttactg actagctttg tttgaagaaa
     1501 cagcagctct ccaaatgaaa ccgatgatga acccagcgat ggtaaagctg atccagccca
     1561 tgcccagcgt gtggagcggt accatggtat tgaagatatt atcgataggt cctaaggaaa
     1621 ttttcgccgt ctggagccct gtgaccaaag ccgggatgag tgtcaggccg atggtccagg
     1681 cgtagacaca ctgccgtcca tcaaagagat tgttgaggaa ggtcaggatg atgatggcga
     1741 cgacgagagg ataaatgaac atcaagaccg gaatagcggc gacgatgatg gtcttcaggc
     1801 cgaacatggc gacgaagaag gaaatgaccg taaagatggt gacccatttc ttatagccga
     1861 tattgccgac gagttcattg aaatacgtag cgcacgacgt aatcagaccg atggacgtcg
     1921 ataagcaggc caggagtacg atgacggcca ggatgacagc cccgacatta ccaaagagga
     1981 tcatagcact cttggaaagg accggtgcgc ctgtttcctg gataccgaaa gcggcgacgc
     2041 tgtcggcacc gatcttggcg atgaagatgt agacaaaagc caggcagctg acggcaatga
     2101 ggccggcctt aaagacttcc ttagtgatag ccgacggttc cgtagccccg tcttccatga
     2161 cgaattcgat gaccaggatg gcgaagacca gcgaggcgat agcatccatc gtgttgtagc
     2221 catcaaggat gccctgtacg gcagcgatgc tggccgttgc ataagcagcg gtcggttcct
     2281 gcggcgagcc gaggggcgta atgaaagatt tggcgatgag cagcaggatt gtgagcagca
     2341 gagccggcgt caggatcttg ccgatacggt cgaccagttt ctgcggcgat acggacagcc
     2401 agaaggaaat gacgaagaac aggaccaaaa agcccgtcat gatcatgtca ttgccaccgc
     2461 tctgcaagaa gggacggacg gcgatttcat aagagacgct gcccgtacgg ggaatggcga
     2521 agaacggccc gatagtcaga taacagatga cnnnnnnnnn ngaaaagacc gtatgccgga
     2581 tggacgcggc ttgccagttc ctgcaagttc ttacagccag aatagcccat ggccaggacc
     2641 ccggccaacg gaaggccgac gccggtgacg acgaagccca ggacagccca ccagacattc
     2701 gagccggcag cctgccccat ggcagccggg aagatcaggt tccctgcacc aaagaacaat
     2761 gcgaacaaca tcaagccaat agcaatcgtt ctgtttcctt tattttctcc
//
LOCUS       DAWABZ010000187         2767 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_7874_length_2767_cov_2.545354, whole genome shotgun sequence.
ACCESSION   DAWABZ010000187 DAWABZ010000000
VERSION     DAWABZ010000187.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7874_length_2767_cov_2.545354"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1099..1108
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          839 a          652 c          621 g          645 t
ORIGIN      
        1 ggtattggca gtcgttaacg acggtgatgt taaagtaggt actcccgttg tttaaggcgc
       61 taaagtaact gcaaaagtat tggctcaggg caaagaaaag aaagtcctcg ttttcaaata
      121 caaagcaaaa tccaactacc gtcgtcgtca gggccatcgt cagccgttca cgaaagttac
      181 aatcgaaaag attgaaggct aatgattacc attacgatgc atcgcgataa gagagaccgc
      241 tacacgggtt ttaccataaa aggccatgcc gattatgacg aagccggcag cgatattgtc
      301 tgtgccgccg tatcggcatt gtcgcagaca gccttgttgg gtctcataca gtatgcttcg
      361 caagacgtac cgtatgaagt aaacgatggc ttcttatccg tccaggtccc caagccctgt
      421 gaagcctcac agatcatctt agggactatg gtagcaggtt tggaacaaat tgtacggcag
      481 tatggagaat acgtcgtact cgactcatag acgtcaggag gtggacggaa tgttcaattt
      541 caacttacag cttttcgctc ataaaaaagg gacaggctcc acgcgtaacg gccgtgatag
      601 cgaatcgaaa cgccttggtg taaaatgcca tgacggcgtc atcgttaaag ccggcaacat
      661 catcgttcgc cagcgtggta cgcacttcca tccgggcacg aacgtaggca tcggcaaaga
      721 tgataccttg ttcgcaacgg ctgcaggtaa agttgctttc gaacgcgctg gtaaatacaa
      781 ccgcaaagtt agcgtatacc ctgtagaagc ataattagct tatagtacaa aacttgtagg
      841 gctcgctcgt gggcgacccg ttagggattt ggatgactct tttcccggag cgggcgcccc
      901 acgtggcgcc cctacagaaa aaaggctgtc ttgatgagac agcctttttt ttatgccaaa
      961 aaaacacttg tcgtcttctc aaaatcctgt ataatagagg atgtatgtac tttgattaaa
     1021 cttaagcgga ggtgttacgc catgcgcgtt agtgtcaatg gagaaaaccg tgagctccat
     1081 gtctatgacc ggtctacgnn nnnnnnnnct ggtcacggac atgtacggcg agttcgtcct
     1141 gaccgaagaa gaatacaacc actggaccga actgctggcc atccagcagg aatcggaaga
     1201 cctgctcttt gaactgaaag acgtcctggt caagcaggaa ctggatgatt acatgtatga
     1261 agaaacgaaa tacatgacga cgaccatcga aacgatccat atggaaaata tctgtatcaa
     1321 agagctgaaa gaagccctgg aaaaaggcga tgaaaaatgg ctgactgaga accatttcat
     1381 caagaccctg aaaaacgtaa caaaataaag gaagtcttat gttgaagaaa atatgctcgt
     1441 ggatcatcgg cgtcattctc accgtcctgg tcctccttat cggtatcttt gctttttggt
     1501 atttcgggtc gacgaactat cacctgacag gcgtcccggt cctgaactac catcaggtca
     1561 ataataaatt caacaccgtg ctgaccatga agccggctaa ctttgacgaa cagatgaaat
     1621 atctgcacga caacgattac cattccatta cgctggaaca gttcgatgcc tacatgcgcg
     1681 gcgaaggcga cctgcccgac cggccagtcc tcatcacctt tgatgacggc tacgtcgaca
     1741 actatgaaaa cgcctatccc atcctgaaga aataccacat gcgcggcacc atattcctga
     1801 tcatcaacct catgaatacg ccgggctacc tgacctggga ccaggtcaag gaaatgtccg
     1861 ccgacggcat ggaattcggg tcccacacca tcagccataa gcccctgacc agctttgacc
     1921 gggctggcgt gcgtcacgaa ctgcaggatt ccaaagacat catcgaaaag atgacaggga
     1981 aaccgtgcca cttcatcgcc ttcccggaag gcaaatataa cgatatggtc atggaagaaa
     2041 cgcggggagc cggctaccgc tacgctttca ccgtagaaac aggccgcgac ttcccctggg
     2101 acgacccgta tgacctggac cgcgtcccca tgtttgaagg ccctatcagc ttcaaacact
     2161 tccggttccg tctgaccttc agcgccttca gcgcgctcct gtggaagacc cataaatact
     2221 ttgaacacat agagatgacc aaagatttgg cccaacatat cccgcagccg taaaaaagtc
     2281 gcagttattc aaaaaaagag cattttatga aacccttgat aagggatttc atggaatgct
     2341 ctttttttta caaaaatttt tgtgtcatat tttgtttcta caatgttaat actatatatt
     2401 aactaattac aaaataaaga ataaaaatat aattacaaac aagagcaatg actgctgcaa
     2461 ataacagata aatcgcgtaa taaagccttt taaaaagaaa aataaatgct ataatgatgt
     2521 cacaaaatac acatattatt tacataaaaa tgcaaaatct tacatgatat atacggtgaa
     2581 atcattatca gcaaaggatg cgcgaacaca tgaataagat atttaaagtt atttatagca
     2641 aaaccagaca ctgctacgtc gtcgtttcag aactggctaa aagtcactgc aaaacggcag
     2701 gtagtcatac ggcaagaaat aaaacagcat tgacggcagc tgtgctgctg gcactgggga
     2761 cgttctc
//
LOCUS       DAWABZ010000188         2757 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_7911_length_2757_cov_3.136566, whole genome shotgun sequence.
ACCESSION   DAWABZ010000188 DAWABZ010000000
VERSION     DAWABZ010000188.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7911_length_2757_cov_3.136566"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          619 a          735 c          766 g          637 t
ORIGIN      
        1 gtcctggtct gcacggacct cctgggcatg agcaatggct tccgcccgaa attcgtcaag
       61 acctatgccg atctccatca gaccatcgtc gatgccgtag gccggtacat ccacgatgtc
      121 aaggaccgca gcttccccga cgaagcccac ggcttcgccg tagacccgga agtcgtcaaa
      181 gccatgaaag aataggggaa aggcagccgt cagcagttag cgttgtaggg gctcgcacgt
      241 ggcgagcccg ccaggtatat gtgagatact aatgaaccgg atttgcttga acgggcgccc
      301 cacgtggtcg cccctacaga aaaagggctt gtcgtcgtgc gacaagccct ttttatacca
      361 tttatttata ttatggaagc acgtcttgct atacagtcat gaagcatatc cagatggtat
      421 attctgtttg acttgtgtat ataatcattg tacaataaac tcaagaaata gaaacgaaca
      481 aaagaggtcg tatgtccgaa cgcacgcgta tcgccgccag acataaacgg gggtatgcgg
      541 cgtttttttc ttgacgacct gacataggag gctatgcttc atgaagcatg atctacatga
      601 cgtgtgtatg cagatacggc gggatacggt gcggtccatt taccgtgccg gttccggtca
      661 tcctggcagc tctctatcgg ctgtcgaaat catggccaca ctttatttta cagatgtctt
      721 tacctgtttt cctgaccggc cccatgcgcc ggaacgggac cgttttgtct tatccaaggg
      781 ccatgcggcc ccggcttatt acagcatttt ggcccataag ggctatttcc ccctggcctt
      841 gctcgatacg ctgcggacgg ttggcagtgc cctccagggc catcccaatt tggaaaaact
      901 gccctgtctc gactgcgctg tcggctcttt aggccaaggc ctgtccattg ccaacggcat
      961 tgccctggcc ttgaaactgc gccatgaaac accggaagtc ttctgtctca tcggcgatgg
     1021 ggaacagcag gaaggccaga tttgggaagc tgccgctttt gcctcacagc accatttgga
     1081 caatgtttgt ctgattgtcg attgcaatgg cctgcagctc gtcgattcct tggtgtcgat
     1141 caagaatatg gaaccgatgg atgcgaaatg ggaaagtttc ggctggcatg tcctccgcgt
     1201 ggatggccac gatacagatg ccctgtacga cgcttatggc gctgccaagg cctatcgcgg
     1261 caagccgacg gtcatcctgg cccatacggt caaggggaag ggtgtatcat atatggaaaa
     1321 tcaagcgaaa tggcacggtg cggcgccgga tgaagcccag taccgcctgg ccatggatga
     1381 actgacaacg gaggtgccgt aatggaacga gtaccgatgc gcgacggcta tgggaaggcc
     1441 ctgctggaac tgtgccgcct tcacgacgat gtcctcgtcc tcgatgcgga cgtcgccaaa
     1501 tcgacgcgga ccgaatggat ccgccaggaa tatcctgaaa aatatatcaa tgtcggcata
     1561 tcggaacaag acctggtcgg tacggcagcc ggcctgtcgc tgacgggctt cgtccccttc
     1621 atttcgacat acggcgtctt tttgacaggc cgggcgtggg gacagatacg caatacggtc
     1681 tgttataata atctcaacgt caagctcggc ggcgcccatg ccggcatttc cgtcggccct
     1741 gacggcgcta cccatcaggc tctggaggac atcgccctca tgcgggtcat tccccatatg
     1801 atggtcatta cgccttgtga ctggtgggaa acgtataagg cgacgatggc cgtctatgat
     1861 atcaaagggc cgtcatatgt ccgctttggc cgcaatccgg cgcccgtcgt caccgataag
     1921 gatacgcctt ttgacctcta taaggcggcc atcttccggg atgggagtga tgtgaccctc
     1981 ttcgccaacg gcctcatggt ccatgaagcc ctgacggcgg cggatgaact ggcccgggaa
     2041 ggtatttcgg cccgcgtcgt caacatccac acggtcaaac cgctggacct ggatacggtc
     2101 tgtacttgtg cggctgaaac gggggccgtc gtcgtctgtg aagaacatca aatcatgggc
     2161 ggtctgggag gggccatctg cgaagccctg gcccagcact gctgtgtccc agtagaactc
     2221 gtcggcatcc aggaccgctt cggcgaatcg gggaaaccgg atgacctcct gaaagcctat
     2281 aatctctcgg ccagcgacgt catccaggcc gtccataacg tgttgaagag gaagtaaagt
     2341 ggctcgtggt tcgtgactcg cagcctcccc ttgagagggg aggtggcctg aaaggccgga
     2401 gaggttaaat ttaataccat ccgctaacaa tggaccagaa agggccgccc tttgggacgg
     2461 cccctacgat gaaataggct gtcgcacgtg cgacagccta ttttgtcatg ctaagtccgt
     2521 atagaatttt gcgatgtatg caaaatcggt aattcgttgt tcctttatcg ttaactgatg
     2581 tataatagga ataactactt tttggaggcg agttatcttg tatacgaaag cgttattgac
     2641 cgacctgtac cagttgacca tgatgcaggg cttatttttt gaaggcaagc acgaacagca
     2701 atgcgtgttt gaccgctatt accggaccaa tcccttccag gggggctata cggtcgt
//
LOCUS       DAWABZ010000189         2714 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_8017_length_2714_cov_3.646860, whole genome shotgun sequence.
ACCESSION   DAWABZ010000189 DAWABZ010000000
VERSION     DAWABZ010000189.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8017_length_2714_cov_3.646860"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          583 a          686 c          728 g          717 t
ORIGIN      
        1 agttcaagag ctgaagggga tgctggagct ggtttcgttg acaggcaata tcgtcagcga
       61 tgatgatgaa acttattttc atcatactca cgctgctttt tcttataaac aggatgggga
      121 acatcacgtt gcggcagggc acatcaaatc gatagaggta ctgtacacgg cagaaatcga
      181 attgcgcccc gttatagaag gacggattcg gcggaaatat gacccagaaa cggggaccgg
      241 cttttgggat ttcgggccag aaacggatta agctggccac tgtcttgtat ctcccgattg
      301 gtcggcactt ctgagaatct tttcacttta tactttattt tcaaatgaaa gtattgacag
      361 aacataccta atagtgtact atttgggtaa acattttata tgtttgatga atgaatatca
      421 atcatccaga gcagttgagg gactggccct atgatgctgc ggcaacctcc gtggggacgg
      481 aaggtgccaa ttccagcggg agttttcccg gcagatgagc gagaaatgga agggattttg
      541 cgcgtcatct gtagctgatg gcgcgctttt tttatggctt tgtcatcggc acgtctttat
      601 ttattttgta aaaggggtgt ccgtatgagt aacgaagtat ctgcacaggc cgtcaaccgg
      661 tcgggcctgt tgcgaagcga agactggtgg gccgtatggc tcggattatt tatcgtcgtc
      721 ctcggtgccg gacagatctg gcacatcgat ttgctcggct gggctgctaa gttcggtgtc
      781 tggatcgacc cgtccaaggc ggtcgggccg gggtcgaaag ggtttgcctg gctcggcagt
      841 atcgggtcgt tagtggcgac gtatgtattt accctggtcg taacgtctat cggtgcgtat
      901 ttcatgggga cgaaggtcaa gaaattcgtc gccggttata ccgtgattta ctggatcacc
      961 atggcggcga cggtcatcgg caattatgct tatctggcag cgacgccgaa taagcgggaa
     1021 gcttttaaca tcggctggtc cttgagtctt ggcgaattgg gctttgtcat tgccctgctg
     1081 gccgggctgg tcatcggtaa ttttttcccg aaagtgacgc gtttcttatc tgatgctgcc
     1141 aaaccggaat ggtacatcaa gacgggcatc gtcattttgg gcatgaatat cggcatccag
     1201 gccatcagtg ccctggggct ggccggtacg gtcatcgtcc gcggcatgtg cgccgtcgtc
     1261 gaagcctacc tgatttactg gcctatcgtc tatttcgtgg cccggaaatt ctttaaattc
     1321 acgccggaat gggcagctcc catggcgtcg ggcatttcca tctgtggtgt cgccgcagcg
     1381 attgctacgg gcagtgccat caaggcgcgc cagatcatcc cgactatcct ggcatcggtc
     1441 atcatcgtct tcgttgccgt agaattgctc atcctgccct ttgcagctgc ttatttcctg
     1501 cccagtgaac cgatggtcgc cgggtcctgg ctgggcctgg tcgtcaagag tgatggcggt
     1561 gccgtcgcat ccggggcaat cgcagatacg ctcatccgca acagtgcgct ccagcaattc
     1621 ggcgtcaatt accaggaagg ctggatcctc atggctgcga cgacgtccaa ggtcttcatc
     1681 gacgttttca tcggcgtctg gtccttcatc ttggctatcg tctggtccat ttaccatctc
     1741 aatgacccgc gggcagccgt cgcatccggg gctggcaagg tttccaaacg ggaaatctgg
     1801 gaacgcttcc cgaaattcat catcggtttc gtcctggccc tggtcgtcat ttttgcagct
     1861 ggctggctgc agccgggcat cgtcgacgct gcgaaagccg gcgccggaca ggctaacctc
     1921 ctgcgggccg tcttctttgg cctctgcttc ttctctatcg gcctggtaac gaatgtacgc
     1981 aagctctgga aatccggcct gggccgtgtc atcggcgtct atgccgtagc cctcgtcggc
     2041 ttcatcatct gggtcggcct gttcatttct tacatcttct atcacggcat tgttccgccg
     2101 acattataac ttcatcatag aaaggattaa ttgctatggc atcgcgaatc acttctgccg
     2161 catcgacggc tgacgaatat tactacaacc aaaccgtaac cccgacagac gctgcgaccc
     2221 atacgggaca gctcgaacgg gatgcagacg aatatttccc cttgcagccc atcgaaaaaa
     2281 aactctgtgc ctattccctg ggattgggcc tgggactgat ggccttgttc atcgtcatct
     2341 ttgaagtctt cggctgaggc ttattatgga aaagggctgt cgcacgaagg tttctgccat
     2401 catgcgaagc ccctttctct ttaaagtttg tagtacgaag cttgtagttt gtagaaaata
     2461 gctttaaatt taaaaccatt cactaaaaac taaaaactgc aaacttaata ctcaaaaaga
     2521 ggctgtctta atgcgacagc ctctttttgc tttcctcgga ttcagatgtg gctatgccgt
     2581 gataaactca tgaaataaac gaagcgcgct ggtcgcaggt agcagactgc aaaaatagcc
     2641 ccaatcgggg cattcatagg ggccgacctt taacctctca gaccgccttt ggcggccagc
     2701 tcccctataa aggg
//
LOCUS       DAWABZ010000190         2710 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_8030_length_2710_cov_3.096045, whole genome shotgun sequence.
ACCESSION   DAWABZ010000190 DAWABZ010000000
VERSION     DAWABZ010000190.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8030_length_2710_cov_3.096045"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          614 a          831 c          680 g          585 t
ORIGIN      
        1 attataatgt attgaaaggc aaattcaatt ttgccgataa agccgtcatg ctcctggaca
       61 tcacgtctgg cggcgtcggc ctgacctgct ggaaatcgga ccgcctctta ttccagcaga
      121 atatccacat cggctccctg cgcatcctgg aaaattttac ggaaaaacag cgtgacgagc
      181 tgacgttccc gacggctgtg cgcgaatata tctacggcac cctttatccg ctctggaaag
      241 gcgtccagca gtatgatatc aagtatatcg tcctgtctgg ccgcggagcc aacttggtgg
      301 cacgcctcat gggcaagacc agtgaaaacg gcctcttcct gatcacgccg gatgaactgc
      361 gccagttcgt ctattccttc cacggcgtca cgccgttcaa gctcatgaag cgcttcggcc
      421 tgtcccagaa cctggccaat gtcatcatgc cgacgattct cttgtattat gaactgctgc
      481 gcatcgtcga tatcgacatg ctggtcgtca tgccgacgac ctttactgaa ggctatacca
      541 tgtattatgt ggccgaaaag acccataacg attatatggc ccatgtccag ggcctgctcc
      601 tggacatgat gcgccgcctg gccaagaagt acttgtccga ttccatccat tccgcccgca
      661 tcgaagaata ctgcaacgtc ctcttccagg cgctgtacca gttcatcggc ctcgattaca
      721 gctataatta tctgctccgc ctggccgcga ttttccacga aacggggaaa ttcatcaata
      781 tccgcaaaca caacctctgc acgtataacc tcatcaggga aacggacctc ttcggcctgg
      841 ccgacgatga aaaggaaatc ctggccgccg tcctctacta tgcctttgac ggtaccttgc
      901 aggaagcgtc gccagtctac cattccctga cgccacggca gaaggtcatt acggctaagc
      961 tgacggcgat tttccgcctg gccgattccc tggacaagag ccatttaggg aaaatcagcg
     1021 ccatccgggc ggaactcagg gaagacgaga tcgtcgtcca ttatacggcc gatgccgata
     1081 tttctctgga acgctggaca tacctgaaaa tggccgtcga ctttgccgaa gtctttggca
     1141 tttcgacgaa attagtgaaa ggataagata atcatggcag attttacgga tgctcaatat
     1201 tttttaaatc gtgaatgtac atgggtcaaa ttcaatgaac gggtcctgcg cgaagccatc
     1261 gtcccgtcca acccgctgct ggaacagctg aatttcatcg ccatcgcagc gtcgaacctg
     1321 gatgaattct tcatgatccg cgtcgccggt gtcaagcacc tcgtcgaaag cggggtcaag
     1381 cacatcgaca tcgccggcat gacgccgcag gaacagtttg aagccatcca gtccatggtc
     1441 cataagctgg tcggcgacca gtaccgctac tatggagata tccagcagaa gctgcaagct
     1501 cacggcctgc acttcatcgg cgtcgatgcc cttgacgatg accagcggct gtggctggaa
     1561 agcttctttg aacgggaaat ctacccggtc gtcacgccca tggccgtcga ttccagccat
     1621 cccttcccat tcctgtccag cctgaacctg aacctggccg tcctcttgcg ccgcaaacag
     1681 ggggaccgct cggtcaagac ggctatcctg ccggtcccga tgtcagtcat cgaccgcatc
     1741 atcaaggtcc cggacagtgt ttcgccggct ggcaagcagc atgaaggtca tcagttcctc
     1801 ttcctggaag acgtcatcgc cgcttatgcg gaacgcttct tcctgggctg cgacatcctg
     1861 gaagtcgaac cgttccgcgt cacccgagac gccgacctgg atatcgacga cgatgccgaa
     1921 gacctcctga tggaagtcga aaagagcctg aagaaacgcc gcaaaggggc ggctgtccgc
     1981 ctggaactgg ctgcttcgtc gtcgcgcatg atccgcgact tcctgcgcga tgaactgcag
     2041 ctcgaagaac gggacatcta ttcgattccg ggtcccctgg actgcaaggt cttcttctcc
     2101 ttcgtcggca tcgacggcta cgatgacctg cgctatccgc cggtgacgcc gcagccgtcg
     2161 tcggaactgg ccgccatcga cgcgcctgat ttctggtcgg ccattgccga acgggacatc
     2221 atagtccatc atccctatga aacctttgaa acggtagaac acttcatcca gctggctgcg
     2281 acggaccccc atgtcctggc catcaaacag acgctctacc gcgtcagcag caagtcgccc
     2341 atcatcgtcg ccctggccca ggccgccgaa aacggcaaac aggtcacggt cctcatggaa
     2401 gtcaaggccc gtttcgatga agaaaataat atccacatgg cccgcaagct ggaaaaagcc
     2461 ggctgccatg tcatttacgg catcatgggc ctcaagaccc attctaagat caccctcgtc
     2521 gtccgccgcg aagaagacgg catccgccgc tatgtccact tggcgacggg caactacaac
     2581 ggcaagacgg cccgcatcta tacggacgtc ggcatcctga cggccaatac cctcatcggc
     2641 gtcgacgcat cggctttctt caacctcttg tcgggctact ccgatcctcc tcagtggaat
     2701 aagctggccg
//
LOCUS       DAWABZ010000191         2679 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_8120_length_2679_cov_3.266387, whole genome shotgun sequence.
ACCESSION   DAWABZ010000191 DAWABZ010000000
VERSION     DAWABZ010000191.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8120_length_2679_cov_3.266387"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          561 a          748 c          756 g          614 t
ORIGIN      
        1 cttaaacagc aaccttctca agaatctccc gcttctataa gcgggagagg ttcaaggcaa
       61 agacttatgt actcgatacg aatgtgctca ttcaggcgcc gtatgccatg cagtgttttg
      121 aagacaatca cgtcgttatt cccctggtcg tcgtcgaaga gctggatggg ctgaaaaaag
      181 cggatggtga acgcgggttc aatgcccgtc aagccatccg ttttttagaa cgcctgcgtc
      241 tccggggcaa tctgctcgac ggagtcccct tggataacgg cggtacgctg cgtatcgaaa
      301 tcaactgcgt ccatgaagcc cttcccgatt ccctgcccaa tgaccaggcc gacaatcgca
      361 tcctcaaggt ctgccgtcat ctgcagcagt gccagcccat cctggtcacg aaagacctgg
      421 tcctgcgatt gaacggtcgc tgctggccct cgatgcgatt cagcgcttat agactcattt
      481 cttcagatat tgactggaaa tggcggggtt ccacgagcag agtcccgccg ttttttcaaa
      541 gcgccatttc tgtcggacgt cgggatattt gtcatgatcc acttcgctga ggaacatgtc
      601 cagaggccgg atgaagtcgc ggtattcgcc atataaggtc ttatagacga cgtaagtctc
      661 gcccgtttcg gcgtcgtcgg ctaaatcgag gatgcggtac atatggttct tgaaatggcg
      721 ccacgtttcg ccgggcctgg gcaggtcgcg gccgaggaca aaggccgtca gagccgcaaa
      781 ggacggcagg aagggtacgg cgtcggccca atcctcgtcg acgaagcctt cgtcgctcat
      841 ctgctggatc aaggctttca tgggctggta atagccatca aagtcgagaa aggcaaagcg
      901 tccttccatc tgatgcagtt tcacctggct catgacctgg ctgatttctt ccagcgtccc
      961 aataccgccg ggaaaggcca ggaagatatc gcctaaatcc atcatctgct gcttgcgtga
     1021 cgtcatatcc ggcgtcacaa tcagtttcgt caagccttca tgctgtaatt cctcatccat
     1081 gaagaactgc ggttccacgc cgatgacctg gcctccggcc tgcaaagcac cgtcagccag
     1141 tttccccatg agcccagtcc ggctgccgcc gtaaacgagg gtgtgcccgt ggcgggcaat
     1201 ccaagcccct aacgcataag cataatcagc ataacaagac cggttcccgc tgcgggaacc
     1261 gagataaacg gtaatattca taagttgcgt cctttctttc ccctaatata tggtataata
     1321 agtgttatta tatcatatat tagggggatt tttcgttatg attttcgttg ccatgattct
     1381 gttaggttcc gtcatcggct ttgtcggtgc tggcggagcc ggcgtcacga ttacgctgct
     1441 gacggtcggt ttcggtgttc ctgtccatac ggccctggcc gtttccctgg cggccatgac
     1501 ttttaccatg atttccggga cggtcagtca ttaccgggaa catgaagtcg atgtgaagcc
     1561 tggggccatc atcggcggca gcggtatgat cggctcgatt gccggcgcca atgtgtccaa
     1621 catcatgccg tcggaactgc tggcgacgat gacgtcgctc atgctcatcg cttccggact
     1681 gattctctat gtccgcctct accaagacgc ctggctgacc tggcactttc ccgtgcggac
     1741 gacagcctta acgggaaaga agctctatat ctacggcatc attaccggtc tcatcgtcgg
     1801 cttcctgtcc ggtgccttcg gcatcggtgc cgcggccttc atccagctgg ctctgatggt
     1861 cgtcttcggc atttccctgc tccacgctat cgggacctgc atgatgatcg tcctgcctat
     1921 ttcggctgcc ggcggcttga gctacttgtt caatggccgc ctggacctgt ggattttcgt
     1981 ccagaccctg ctgggcctga gtctcggcgc ctatataggt gcaaagttca cgcacttagc
     2041 ccctaagccc ctgctcaagg cagctatcgt cgccatgccg accctgggcg gcatcatcat
     2101 ggtcctcttc ggccgctaag cctgctgatg gcggtgccag cagtagcaga tcaggaccgt
     2161 caggatgacc cagaacatca gccgcccgct gtgctggctg aggtggatga ggtcatggcc
     2221 taaaaggact tcgatgacga cccgcgggac cttgccgaca gccgtcgcca ggaagtggtc
     2281 tttctggctg aggtggctga cggcggcgat agccgtaaag gggacgttgg ggatatacgg
     2341 aatggaccgg gccatggcca gcgtcgagaa gcggctgtat ttatcgagct gattccgcaa
     2401 gtgatggcgg gagatgacgt ggagggcctg gcggcgcagg accgtccgca agatgaggaa
     2461 gctcagttcc gtccccagga cttcgccaat ccaggaaatg ataaggcccg gcacgaggcc
     2521 gaagatggct ccgttgacgg tcagaaaggg aatcgacggc agaccggtca tatcggtgac
     2581 ggtaatcatg aagatactga tgagggccgc atagatgccg aaagatgaaa tataatcgat
     2641 gaggacgtcg acatcaccgg ccaggctcag gcggaacag
//
LOCUS       DAWABZ010000192         2639 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_8246_length_2639_cov_3.247678, whole genome shotgun sequence.
ACCESSION   DAWABZ010000192 DAWABZ010000000
VERSION     DAWABZ010000192.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8246_length_2639_cov_3.247678"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    983..992
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          657 a          687 c          621 g          664 t
ORIGIN      
        1 aaagtgtaaa ggatgtcctt gcacaaaatg taaacgatgt tattcttgac acacgggtga
       61 cccctacaaa ctccgtacta taactataaa gagaaaggcc cttgcattat ggctgtattt
      121 tgaaaagttt ttctgtattt gctttggcat gagctgtcaa ttcttcgaca gatacgccca
      181 tatacccggc cagggtctgt gccacatagg tgatattgtt gggcacattg gtccggggca
      241 ggtggaagcc ccgcggcgtc aggtacggtg catccgtttc gatgaggacc cgatccaaag
      301 gcaggatgga tacagcatcg cgcaaaggag ccgcccgccg ttcatcgcag atccagccgg
      361 taataccgat ataaaagcct aaatcgagca tcttttccag cgtcttcttg tcgcccgtat
      421 aacagtggac gaccgattgc gtgcaaagcc cttcatgccc cttgaaacaa gccaggaagt
      481 catcagctgc gtcccgttca tgaaggaaca tgggcttacc taattcttcc gccatagcaa
      541 tgagggcttt aaaaaagtgg agctggttct ctttcttaga atacatgcgg tcgtaatcaa
      601 gacccgtttc accgacagcg acgactttcg gattcgtcgt gatgatgcgc cggatttcct
      661 ccaaatccgc ctccgtcgct tcatcagcca cgtgaggatg gataccggcc gtgccaaaag
      721 ccgtgtgatt gcggacaaac tgatcgacca gacggttctc ccgggcctcc gacccagtca
      781 ggatacagca gataccgtcc gccaccgcat cggcaataat tttttccggt ttgggaaaag
      841 accgggtaaa taaattgagc ccaatatcat aatattttat atccatgtca gacacctcgt
      901 gaaaataaga ttccacgaat attgtaccaa aaaaacaggt tcctgcgtag cttaaaaaaa
      961 gcttgtcaca caacgacaag ccnnnnnnnn nnattccttc tcctaagtta cacatgactg
     1021 ctaaatcccc ctctccttct tgtgtcaaat caattccaaa atcattatgc agggttgtaa
     1081 tcttttcact ggctttcttt gacgataccc attttgcctc attgtaccaa attttgattt
     1141 tttttgtaca atccacattc atggcaaaaa aagagcaccc aggaaatttt cctaggtgct
     1201 ctctgtccag taatctggcg gagtgagaga gattcgaact ctcgcaggcc ttacgacctc
     1261 taacgattta gcaaaccgtc ctcttcagcc tcttgagtat cactccatgc gtcaactgac
     1321 tatgtcagta tactacgatg gagcgatatt gtcaagttat tttttcaata tatcttctac
     1381 ggccttggtt ttattgttca tcatattcgt atagcccggt cggatatcgg ctttcaagac
     1441 gacttcagcc cgcaggccct ggtccgtcag gtattgcgtt gcttttttga cgactgccat
     1501 gacatcgtcc cattcgcctt cgatttcggt gaacatggaa ttggtccgat tcggtaagcc
     1561 tgattcccgg ataatacgca cgatttcagc gacataaggg gcgaattcat cgccagtccc
     1621 gcacggggca atagctacag caattaaggt attcatgata ttatgcctcc tctacagtca
     1681 atgggttctg ggcaatcgca tcgggcgttg ccacatagag ttcgtcgatg aaatccgtct
     1741 ggaaagaacc tgtccctttg gcatgagctg cggcccgtgt accggccagg ttatagacgg
     1801 cagagccggt aatggcggcg atgaacggat ctgctacggc tgcatagaca gccatgacac
     1861 cgccaaggga acagcctgtc ccggtaatgc agggcatcat ttccgaaccg ccctgggaaa
     1921 agacgagctg ccggccatcg gtaatcaaat cttcttctcc cgaaatggct acggctccgc
     1981 cagtccagcg ggctacagcg acggcagccg gagcggcaga acggacagaa tcggtagaat
     2041 cgacgccgcg aacgtccgaa gtatcagaac cgccttctaa gccccataat ccggccaagg
     2101 caatgatttc agaggcgttg ccccggataa tagacggttt atagaccttg aaggtcgtca
     2161 aaaggtccgt ccgcatttgc cccatgccaa tggccacggg atccaggacc cagggtttgc
     2221 cttcttcatg gagtacggct gccgtttttg gcagtgtttc cgcataaatc gggaataacg
     2281 tccctacgtt gatatacata gatgcaccgg ccttggccat actgacgcct tcgtccggta
     2341 aatagaccat agcggccgaa ccgccgacag ccagctgggc attggcaacg aaattaatgg
     2401 tcaccgtatt cgtaatagac ggcgtcagcg gattcgatgc cttagccagt ttcgcggctt
     2461 cccgaacggc atcgcggatt tcagattcag tcatattatt accacctttg tcattttgct
     2521 gtttctatta tagacgttct gctggtgaaa gactagaggg gatttagtag catatccaca
     2581 atccgagaca gttagcggtc agcagttaat tgtccgcagc cttgcggact acaccgtag
//
LOCUS       DAWABZ010000193         2610 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_8325_length_2610_cov_2.921331, whole genome shotgun sequence.
ACCESSION   DAWABZ010000193 DAWABZ010000000
VERSION     DAWABZ010000193.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8325_length_2610_cov_2.921331"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          585 a          684 c          679 g          662 t
ORIGIN      
        1 cctctccggc cctccgggcc acctccccta ttaggggagg ctttcacgaa acacgaacta
       61 caaacttttc ttacccatta taattttccg gatagatggt gatatcgtag tggttcttgt
      121 cccattcgtg ggtaatctga tgcttgtacg gcgacagggc gttgatgagg acttcataag
      181 ccttggtgcc ctgatagatg ccgtcgtcgg agatgtcgac tttatagcca ttttcgtggg
      241 aatagatgcc gctggcatgg tagccgcttt ccgcaccgcc ggtgatgacg aaggaaatgc
      301 cagcctgacg ggcttcggta ttcagatgat ccaggaaagc gatggtcgag ctctggaaat
      361 gagccgtatc ggtcgtccag ttgctgtccc acggcgtatc cataccttcc gtgctgctcc
      421 atttgcgcag gttgctcttg ccgaaatcac ggcggtatgc gtaggcatcg gcaatagctg
      481 catcatagga gtcgtgctga tactgatact ggtattggta ttggtcttca tatttctgac
      541 ggttccaggc cgtaaccggt gtcgccatgt gctgcgattc agcataatgg tacttgaccg
      601 tatccggcgt aatcgtaccg ggattatcaa tattggcaaa agccgaggcc ggaatcaggg
      661 catggacgcg atcccgaagg cggtcaccga ttttgtcaga attattcgtt gtggtagtgt
      721 tcgttttctt cttggctttg tcttgttcct ggacctgtgc cataacggca acggccggag
      781 ataccgtttc cgattccttg acctgtgctg ccgaagcagt gacagttccc ataagacagc
      841 aaagaaacag gattgcgatg atagacttct tcattgaatt cccccttaaa atactgaaat
      901 tatgtgtatg acattcggtt attttcacga acagcgtttc aattgtaaca tattcagggg
      961 aatttgaaaa gtcctgctac gaacccagga gctgacgctg ttccatttcc tgggctgccg
     1021 cctcatcctg gtcggccttc ttcgtgtctc ctaatttgcg ccagacctgg ctgcgcagct
     1081 tgtagccatc ttcatagggc ttgacggcca aagcctgtgt caaatactgt acggcccgcg
     1141 gataatcgcc ctggtccatg gccagctggc ccaggctgtc gtagacggcc aatttatcgc
     1201 tgtcatccgt ggccaggcgg accgattggt catagtcttt tttagccaaa tcgaaacgct
     1261 tcaaatggcg gtaacattcg ccgcggttat tgtaatccat agccgtttcc ttcaaagcca
     1321 gggctttcgt caagtcggca atggccaact cgtcctggcc cgtctgggcg tagaccaggc
     1381 cccgttcgga atagacccgg ctgtcgtcgg gatggcgttt caccgcttca ttgagcagtg
     1441 ccagggcctg gtcgtatagt ccggcttctt cggcgacgga accggccagg aagtaatcgc
     1501 cgcggtgcat gaattcatac atgacgccga tacggcggct caggtcggcc attttcttgt
     1561 cgcccttctt atcatagatc aggctttcga ggatatagtt ctgaggaaag gccggagcca
     1621 gcttttcccc ctgtttgacg tcatccaggg ccttgtcata cgattcgatg gccgtatagg
     1681 cccgggcccg gttgaaatac atcagtccct gcaaaggatc gcccttctgg ctcaaggcca
     1741 gggccttcgt atagtcgttg acggccagcg ggtattcttt gcgctgttcg taaatggcgc
     1801 cgcggttgaa ataatggaag gccgtgcccg gttccaggga aatggcttta tcgcaatcgg
     1861 ccagggcttc gtcattccgt ccgaggaggc tgtaaatcgt gctgcggcgg tcgtaagcat
     1921 cggcatcatc gggatgttct gcaatctggc cgttgacgat ctgaagggct ttctcataat
     1981 ccttggcgcg gacagcctgc aagagttcac tttccccgtg ggtctgcaaa gcaagggccc
     2041 cttggccatc gcctttcacc agggtcacat cgtccgccag ggacaggcag ggaacggcca
     2101 aaaagcagga aatcagcccg gctaatatgc actgtttcaa tcggttcata agaatccccc
     2161 tatctctcga attttgcttt accttataag attcgacata gccccgccaa attcctgcca
     2221 aaagacaaaa agtaatatgg tataatgaag aaaaaaaaac gaatgaggtg agcaatttgt
     2281 ttgaaaaaga atatgaagcg tcagcccaac gtagccggtg ccatcttctt cgtatccatc
     2341 ccctacggcc tcgcgtcgcg gcaccgagcg taagaggcag tttttataat tataccagcc
     2401 ccgtcccccc tctccatcgc cagggccgtc atttatccag tcaaaacatt gccggcttcc
     2461 ctattcaatg ctaaaatgga agaaatacaa cccaaaaagg aggcctcata aaaatgttta
     2521 agtatacaga acctcaaaaa cggctcataa ccctggccct ggccgggctg gtatggatag
     2581 gaggcaatgc catggcgatg aatgtagatg
//
LOCUS       DAWABZ010000194         2587 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_8406_length_2587_cov_2.760269, whole genome shotgun sequence.
ACCESSION   DAWABZ010000194 DAWABZ010000000
VERSION     DAWABZ010000194.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8406_length_2587_cov_2.760269"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          519 a          699 c          785 g          584 t
ORIGIN      
        1 gtatgaggga tgatggaaac ggaaagttgg ctgctgccga cggatacgac agtcaggctg
       61 atgccgtcga tggtgataga gcctttttcg acgatgaacg gctccatgtc cttggggatg
      121 gaaatggtga tgacccgggc aatgccatcg gtggcaatgc gggcgacgtg gccgatgccg
      181 tcgatatggc cgctgacgat atgaccgccc aggcggccgc cgacctgcat ggcccgttcg
      241 aggttgactt gggaaccgct cttgatagtg ctgaacgacg agcggcgcat ggtttcatgc
      301 atgacgtcgg ccgaaaaggt cgagtccgtc atatccgtga cggtcaggca gacgccgttg
      361 acggcaatgc tgtcgccgag ctgcgtgcct tctaatactt tgtgcgcctg aatggtcagg
      421 cggatggaat cggggcggcg gtccatatgg gcaatcgtcc ccagttcctc aatgatgcca
      481 gtgaacatag gcgccctccc tgttggcatt atatgccgta atcaatacat tattgtcatg
      541 gagttctgcc gaatcataga ccagcggcag gcattcatcg agttcatcga cgccgcagcc
      601 gccgacagac ggcagggctc cggtcccacc gatgaccttg tcccccatga aggcatagat
      661 cttatcgacc aggcgggcat cgaagaagct gccctggacg gtactgccgc cttcgacgag
      721 gatgctggtc agcccttcgt cgtgcaggag ctgtaaggcg tcgggcaggt agatggtctt
      781 gccgtcctgg agcggtgcca ggatgacgcg gacgccagcc tctttcaggg ctttcatctt
      841 ctctaccggg cacagggacg tcgtgacgat gatggtctgc gtcgtcttat cggtgacgac
      901 gagcgaattc aaaggcgtcc gccctttcgt atccagtacg atgcgcacgg gctgatgcgg
      961 tgcatcctgg ccgggccgtt cgatgcggct ggtcaagagg ggattgtccg tcaggaccgt
     1021 gttgatgccg actaagatgc cgtcatagat ggagcgcaga tagtgaccgt agctgcgggc
     1081 ccattcgttg gtgatccact gggatttccc cgtccgtgaa gctgtgcggc catcgaggct
     1141 ctgggccagt ttgatggcga tgaacggttt ctggaccatc atgttcttga tgaagacttc
     1201 gttgagctga gccgcttcct tcgacatgag gccgacttcg acggcgatgc cggctttgcg
     1261 caggatggcc gcgcctttgc cagccactaa ggggttgggg tcgagcatgg ccatgacgac
     1321 tttgccgacg cctttttcga cgagggtccg ggcacacggc ggcgttctcc cgtaatgggc
     1381 gcaaggttcg agggtcacat agaccgtagc ccccttcgcc cgttcgccag cttcacgcaa
     1441 tgcccatact tcggcatgag gcgtgccggc cttatgatgg tatcccgtgc cgacgatttc
     1501 gccatctttg acgatgacgc aaccgacggc aggattgggt gtcgtatagc ccagggcggt
     1561 ctttgccaat tccagggctt ttttcatata ctctgccggt tccaatgtat tccttccctt
     1621 ctattcagat tcgcaagata aaaaaaggct atggacgtaa gattcccata gccttttgag
     1681 cgcaacaaaa acacaatctg tcgtcttttc ccatccagac tgtactgtcg gtaccgaaat
     1741 tgcatcggtt caacccttgc gggttagcgg actgtcaccg ccggtcagga attgaaagcg
     1801 ccgctttctc accttgcctc aaagactatc cttattcatt tgaactacat gtatgatttt
     1861 agcacatgaa aagaagaaat acaagtcctt atcgcaagga cgcgatggca gcggcccggt
     1921 cgtcagctcc gaagacggca ctgccggcga cgaggaaggt ggcaccggcg tctttgacgg
     1981 cctggacggt cttggtattg acgccgccat cgacttcgat gacagccgtc gtattgccgg
     2041 cctgttccag aagcgccttg gcctggcgga tcttatcgac ggcataagga atgaacttct
     2101 ggccgccgaa gccggggttg acgctcatga tgaggatcat gtccagctgc ccggccaggc
     2161 aggacaaagt cgatacgggg gtcgccgggt tgagggcgac gccgcctttc atgccggctt
     2221 cgtggatctg ctgcaggacc cggtcgagat gggtgcaggc ttcctgatgg accgtgacgt
     2281 actggacacc ggcagcagcc aggccgtcga tgtaggtttc cggatgttct accatgagat
     2341 gggcatcgaa gggcagcttg gtgcagctac gcagagcctt gacgacgggg acgccaaagg
     2401 tcaggttcgg cacgaaatgg ccgtccatca agtcgagatg gatacaatct gcccccttcc
     2461 gttcgacgtc ctgaatgtct tcagccaggc gggagaagtc cgccgataat atagatgggc
     2521 aaatggttac catgactatc gtttcctttc tgcaatggaa gtgagtatct tacagtatgt
     2581 gtcatac
//
LOCUS       DAWABZ010000195         2554 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_8527_length_2554_cov_2.803922, whole genome shotgun sequence.
ACCESSION   DAWABZ010000195 DAWABZ010000000
VERSION     DAWABZ010000195.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8527_length_2554_cov_2.803922"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          577 a          640 c          745 g          592 t
ORIGIN      
        1 atctaccatt ataccgtagc cgaggtgaaa aggctaagac agaaaagaca taggggggat
       61 ttcgcaggca tcgaacctgc gaaatccatc gccattcatc ccctacctaa gaggaaggag
      121 tcttctggca aaactgggat aaagaggtta cttcaatatc ttgttattgt agcgccggat
      181 gtagacgata tattgccctt tcgcgtcttt ctggccgtca aaggcgacag tgatcgacac
      241 gtcatcctgg gtccattcga cttctttatc actctgtttg actgggaagt tttccatatt
      301 ggaataaatc gtcgctacca ggcgggaata aatgctcttg gctgtcgcct tgtcagtcgt
      361 cttaaagtaa ttatcgaaag ccaggacctt gcccttggcc gacgtatcgg ccgagaagat
      421 ttcgatgcct tccgtaacgg atacgttctt gacgaccgac gtccgttcag cccgttccgt
      481 aaaggatacc gtgctgccgt agaaagtcca gtcgggcagg atggagaaat tggcatcgaa
      541 atcagcccgg ggcataccga gatagaccgt ctgatacgcc gtgagcgggc tttcggcgat
      601 ttcatcgaca gactggttgt agtcaaaagc accggccggg gtcgccaatg ccgcccataa
      661 cagggttgct gccattaatt ttttacaggt catgagaatt cctcctttac aggaatggcg
      721 tcaagatcaa gctgaacaag gcgaagacca gacgctgcaa gggaatgagg atatacgtga
      781 aaaaaggcgt catcatcagg gcgatgagga tgaggaagct gtagcgttcg aggctggcca
      841 gcttatagtt ccacgacgtc ggcaggaaac acatgaggac ccgcgagccg tcgagaggcg
      901 gcaagggaat catgttgaag atggcaaagt tgacgttgta caagacgatg aggtgaagga
      961 ccatgggcag ggccgtcgtc gtaaagagat ggagcttgac aaagatgact tcaatgacca
     1021 aggcgacgaa agccgtgatc aggttggaca cgggaccagc cagggataca cagatttccc
     1081 ctttccgcca gttccggaaa ttgccgactt ggatcgtaac cggcttggcc cagccgaact
     1141 gggcgacgag cagcatgatc aggccgaccg ggtcgatatg agccacgggg ttcaacgtaa
     1201 ggcgccccat cagacgcggc gtatcatcgc ccatccagtc tgccacctgg gcatgggcgt
     1261 attcatggat gaccatggcg atgagcaggc cgggaatccc ggcaataata gaaagtatat
     1321 taaaatcgaa catagaattc tcctttcgtt aatatttcta ttatacggta tctaggagaa
     1381 gataacaaga tgtggctagg ggctagggat taggggctag gggctagggg tcaggggtta
     1441 gccgctgact gctgagtgct aaaaaaggcg ctgtcttcat gcgacagcgc cttttttcac
     1501 cgcttccggt attcatattc tacgttttgt tcgacgatgg ccgctttttc ttcgatttcg
     1561 cggatttcgt tgatcatctt cgtcacgaag gtttcatcct tgatggattt caggttgatg
     1621 cggacgctgt aaaaagccga gcgcatggct gccctggcgt tcatggtggc aatgacgccg
     1681 tccgtgatga cccaggcgtt gccgtagcgg acgacttgtt ccgccagctg gaggaggacg
     1741 aagatgtcct tgcccagttc aaagggcgac agggccgcat ccttgaaggc ctgctggacc
     1801 ttggccgccc ggtccggcgt atccttgggc aggcggacgg cgcggatgac gccatcgaag
     1861 gccgccgcgt cttcagcgat gccgtcgagg taacgctggc gaaggccgtg agccttattg
     1921 cggatggata ccatgagttt ctcgatttca tcgtagccct tcttgccgat ggtcaggttg
     1981 gcgaccatct ctacgagcgc tgctgccgaa gcagcggtca tagcggcgat gccgccgccg
     2041 ccgggaacgg gttccttgga agccgtgact tccaggagcc cgtcgagggg catggattgg
     2101 aattgtgcaa aatccatctt agaacaatcc cgtaatgatg ccattttcgt cgatgtccat
     2161 gcggttggca gccggtactt tcggcaggcc cggcatggtc atgatattgc tcgtctggca
     2221 gacgatgaag cccgcaccgg tgcagacgcg gacgtctttg accgtgacct tgaagccctt
     2281 cggtgccccc aggagcgccg ggttatcgga cagggaatac tgcgtcttgg ccacgcaaat
     2341 cggcatttta tcgaggccaa aggcttccag ttccttgatc tgtttctgag cgttgccttc
     2401 aaagaggacg ccgtcgccgc cgtagacttt cttgacgata gtttccagtt tttcaggaat
     2461 cgaagcgttg acgtcgtaga gcggtttgta cggagtcttc ggctgctgca gcagtttcag
     2521 gactttttcg cccatttcga cgccgccttt gccg
//
LOCUS       DAWABZ010000196         2546 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_8555_length_2546_cov_3.081092, whole genome shotgun sequence.
ACCESSION   DAWABZ010000196 DAWABZ010000000
VERSION     DAWABZ010000196.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8555_length_2546_cov_3.081092"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          666 a          619 c          645 g          616 t
ORIGIN      
        1 ccctttttgt tgttagtagc tagcagttag ttgttagcgg atggctttaa atttaagggc
       61 tttttctgca aacgacaaac tctgtgctac aaactgtaga gtaaaaggga ctgcgcatga
      121 tgacataatc ttcgtgcgac agcccctttt ttgtcattgt aggggctcgc acgtggggag
      181 cccgttccgg aatttggatg attcttctga cagactagtt gggaaaaata acacatatcc
      241 tgaagcgggc cgccctttgg gacggccact acgaaatact aaccactagc cactaaccac
      301 taaccacttc tcacggttgc tttttcggcc atttctgata gaatgatgat aaatagtata
      361 gtatatatcc gtttgtcatc cgcttatttt ctgtcagagg aggttttcat catgggttac
      421 attttaaaga aagaccagca ggacctggtc aatttggcta aagattttgc tgaaaagaaa
      481 gtcgctcctt ttgttcatga attggatcag gctaaggaac cgggccagaa gttactggac
      541 atctatcagg aagccgtcga catgggcttc tgcggcctgg aaatcccgga agaatacggc
      601 ggcctgggcc aggattatta taccgttgcc gctgtctatg aagaactggc aaaagtcgac
      661 gccggtgtcg ctacggctat tgccgcttcc ggcctgggcc tgaaaccggt actccagcac
      721 ggcacggatg cccagaagaa gctctatgcc gaatacctga ccggtgaaaa gggcagcggt
      781 ttctgcgctt tctgcctgac cgaagccaat gccggctccg atgcggcagc cgggacgaca
      841 gtcgctgtca aacagggcga tgaatacgta ttgaacggca cgaaatgctt catcaccaac
      901 ggcggcgtcg cttcggtcta cacggtcttt gccattaccg acaagacaaa gggcgtcaaa
      961 ggcatttcgg ctttcatcgt cgaacgggac cgcccaggcg tttctgtcgg caaggaagaa
     1021 gataagatgg gcatccgcct gtccaacacg acggaagtca ttttccagga cgtccatatc
     1081 ccggctgacc acctcatcgg tcaggaaggg aagggcttca tctatgccat gcagaccctg
     1141 gacctggccc ggcctatggt cgcatccctg gccgtcggca ttgcccagcg ggctattgac
     1201 gaatcggtca aatacgccaa acagcgcgtc accttcggca aaccgattat caagcacgaa
     1261 gtcatcgcct ttaaactggc tgacatggac atgaagaccg aagtcgcccg tgcggcaatc
     1321 atcaacttcc tcaacaccta ctatgccgta ccgaaacgga actatacccg cgaagctgcc
     1381 attgccaaat gctttgccgg tgacatggcc gtccaggtcg cattggaagc tgttcagatc
     1441 cttggcggct tcggttacag ccgggaatat ccggtagaaa aactcgtccg cgatgccaag
     1501 atcatgcaga tttacgaagg caccaacgaa gttcagcgca tggtcattgc cggcttcgtg
     1561 gcgaataaat aacactcatc aatgaagggg gaaccaattc atgagggatt tgaacattat
     1621 acgttctaaa aaaggtttta tcagtgatat ggacggtgtt atctaccaag gcagcacctt
     1681 gattccgggg gtaaaggatt ttgtgaactg gctccagaaa gaaaagaagc agtttctgtt
     1741 ccttaccaat tctagtgaac ggaccccgct ggaacttcgc aaaaagcttc agtctatggg
     1801 actggatgtc gaggaaagtc atttttatac gagcgccctg gcgacggctc atttcctgaa
     1861 aacccaggct ccaggctgtt cggcttacat catcggagcc catggcctga tcaatgctct
     1921 gtatgaagtt ggtattcctt tcaatgatgt caatccggaa tacgtagtcg tcggcgaaac
     1981 gacaggctat aattatgaaa tgatcatcaa ggcaaccgag ctgatcaaca aaggggccaa
     2041 gcttattggc accaatggcg atatgaccag tcctagtgac cggggcgtta tcccggcctg
     2101 ccgggccttg attgcaccga ttgaactggc tacggggaag aaagcttact tcatcggcaa
     2161 gccgaatcca ttaatgatgc gcactggctt gaaaaagctg ggcgtccatt ccgaagaagc
     2221 cgtcatgatt ggcgaccgga tggatacgga catcatcggt ggggtagaat caggtatgga
     2281 aacagtcttg gtcatgagcg gtgtctctaa tcgggaaaat atccagcagt tctcttacca
     2341 gccccattat atcctggacc ggatcggtga tttagtgccg aaaaaagcaa aagcttaatt
     2401 aaaaaaggac tacgcacgaa ggtttctctg ccatcatgcg tggtcccttt tgtcttatag
     2461 tttgtagtac ggagtttata gtttgtagaa gatggtttta aatttaaaag ccttcactaa
     2521 aaactacaaa ctgcaaactt aatact
//
LOCUS       DAWABZ010000197         2493 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_8736_length_2493_cov_2.455291, whole genome shotgun sequence.
ACCESSION   DAWABZ010000197 DAWABZ010000000
VERSION     DAWABZ010000197.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8736_length_2493_cov_2.455291"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          653 a          704 c          665 g          471 t
ORIGIN      
        1 aggggagctg gccgccaaag gcggtctgag aggttaaagg gcggccccta cgcactttat
       61 gctatatttt tcgcgctata tatagtataa ttatgtcgag tagttgtatt ttatatttcc
      121 ttattaggca atgaacagga gttgttacac atgaacaaga aatgtttagc tctggcagcc
      181 gctgccatgg cggctgtgac actggtcgcc ggctgtggtt cttcagcaga caaaaaggac
      241 ggcgcttcgg gagatcagaa gaccctcgtc gtcggtatgg aaccgacgtt cccgccgttc
      301 gaatttacgg aaaacgacaa atacatcggt ttcgacattg atttggccaa tgccatcggc
      361 gaaaaaatgg gcacgaaagt tgaagtcaag agcctcggtt ttgacgccct catcccggcc
      421 ctgcgcagcg gtcagatcga catgattgct tccggcatgg acgctacgcc ggaacggcag
      481 aaacaggttt cctttacgga accgtatttc caggatggtt attccgtcgt cgtccgcaag
      541 gataacacca acatcaacgg cttcgatgac ctcaaaggcc gcacggtcgg ttcccaggtc
      601 ggcacgaagg gcgtagacct ggctacggaa gccggcgcta cggtcaagca gtatgacgcc
      661 aacagccagg gctggatgga actgcagagc ggcacgtgtg atgccgtcgt catcaacacg
      721 tcggtcgccc tctactacat gaaagaaggc ggcgataaag acctgaagat cgtcggcgac
      781 gccaagaaag ctgatgccgg tatcgctatg gctgtttcca aggacaaacc ggaactcctg
      841 gaaaaggtca acaaagccct ggccgacctc aaagccgacg ggacttatgc caaactgtac
      901 aagaaatggt tcggcgtcga tccgaaggca taatcgatgt ataaagccta cgtattcgat
      961 ttcgactaca ccctggccaa ttcggaaaag ggtatcgtca tgtgcttcca gctcctcttc
     1021 gaggccgaag gctatgacgg tatccctgaa gaagctatcc gccgcacgat tggcatgacc
     1081 atgtacgacg ccatggccct cctgaccggg gaaacggacc cggaccgggt caacgccctg
     1141 atttgggaat acaaggtccg cttctccgac aagtacatga cggccaacac ccatctctat
     1201 ccgcagaccc tgccgacgct ccatgctttg aaagacgccg gcgccctgtg ttgcaacgca
     1261 tcgaccaaga cccggtcgcg tatcggcgaa accctgcgga aataccagat ggaaagcctc
     1321 atcgacctcg tcgtcggcgt ggaagacgtc gccaaggcca aaccggcacc ggacggaatc
     1381 aacgccgtct gccgccactt cggcctggat aaaaaggacg tcctctacat cggtgacaat
     1441 accatcgacg ccagagccgc caaaaatgcc ggcgtcgcct ttgccgccgt gacgacggga
     1501 acgacgacga gccaggaatt cgcccgcctg ccccatgtgg caatcatgaa ggatctcagc
     1561 gaaatcctta ctattcccta attggaggaa ctcattatga tgaagaaaaa agttacgacc
     1621 ctcgtcgctg cctcgctggc agccctgacc cttctcgccg gctgcggctc caacggcaca
     1681 gccgataaaa aagatggcgc agccaaaggc gacaagaccc tcatcgtcgg cacggaaccg
     1741 acgttcccgc cttttgaatt cacggaaaat gaaaaggacg tcggcttcga catcgacctg
     1801 gcccaggccg tctgcgataa gctgggctac aagatggaag tcaagaacct cggcttcgac
     1861 gccctcatcc cggccctgaa gagcggccag atcgacctca tcgccgccgg catggatgcg
     1921 acagatgaac ggaagaaaca gatcgacttc acggacgtct attaccaggg cggctacacc
     1981 atcgtcgtcc cgacgggcaa tacggacatc accggttatg actccatcgc cggcaagacc
     2041 gtcggcgccc aggtcggttc caaggcagcc gattacgcca gagaacacgg ggccaacgtc
     2101 aaggaattcg acaccaacac ccagggctgg atggaactgg aagccggtac gtgtgatgcc
     2161 gtttccatcg ataaagccgt cgcccagtat tacctccagc agggcggcaa agataagctc
     2221 aagatcgtcg gcgaacccat tacgtcccgc ggcgtagcca tgggcatcag caaagataaa
     2281 ccggaactga gcaaacaagt caatgaagct ttgaaggaaa tcaaggcaga cggaacgtac
     2341 gctaaaatct ataagaaatg gttcggcgaa gaaccgaagc agtaaaaaaa aggcttgtcg
     2401 cacatgcgac aagccttttt aagtggttag tggctagtgg ttagcggatg gtttcaaatt
     2461 taaaccatct tctacaaact ccgcactaca aac
//
LOCUS       DAWABZ010000198         2490 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_8751_length_2490_cov_2.754825, whole genome shotgun sequence.
ACCESSION   DAWABZ010000198 DAWABZ010000000
VERSION     DAWABZ010000198.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8751_length_2490_cov_2.754825"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          629 a          613 c          729 g          519 t
ORIGIN      
        1 gacaagggtc cctatctgat ggtcaaggca gaaattaaca atgtaacggg ctgtcttgcg
       61 gaggatgtcc tgcatccgga aatggcgttt cttcgctaac tgacagagct agaaactgtg
      121 ataaacaaaa aaatgccgat agttattaca actatcgaca tctttcgtct acatctcttc
      181 gctatatagt tatgacttca caatgcaaaa agtctggaat gttttccagt gtttaaccaa
      241 taatatcatg aattgggatt tcattccaaa gttaagttca gttttcttcc ttctcttcat
      301 cctcatcgac aatgacgctg aggatgttgg ctttggtatc gatgatgcgg ctcagggagc
      361 ggtcgtattt ccggacggcc agagccgaga cgatgagtcc ggcgatgccg ccgacgacgg
      421 caccggcagt atggcctatg atgagatagc ctaaaagggc cgtgatgagc agcatggcga
      481 cgggcaggat gaagcagaca aaggcaccgg aaatgaggtg cgagtcatcg acttcatagc
      541 ggacgtggtc gccttcggca gcgccgatgg aattataggc cagaagcttg acgtgatccg
      601 ctgcggcgca gacactgcag gccacataaa gcgaatcgcg actgacccgt actttcgcca
      661 agccattatc cttgatttct tcgacaaaac cttcaccaaa acgcatatct tatgccccct
      721 tccgaattag taatcaataa taattatttg ttaatttgat tatatcataa tatacgttat
      781 taatcaagtg taggctgatt gcatctcatc acttgaggag ttagcagtta gtggttcgta
      841 gctaggggct aggggttagg ggttaggggg taggggttag aggctagcgg ctagcggctg
      901 gaggctagta gctggcggct agtagctggc ggctggtggc taacggctac cgctcactca
      961 gcaggccccg gacgatgtgc ttgcggcagg actggtagac gaaggtcagg acaatggccg
     1021 tgaccagcca gctggccggg taggccgcca tgaggaccag gaagtcgcgg tgagcggcaa
     1081 atacggtgta cagccaggtg atgcggacgc cgcagatacc gaccaggacg acgacagccg
     1141 gcggcaggga atatccatag ccgcgcaggg atccggagat gatgtcgatg atgccattga
     1201 ggtagaacgt cccggctacg taaaagaggc gcttcgtccc caaggcgacg acgttcgggt
     1261 cgccgttgaa caggcggatg agttccggcg aaaagaagag gaccggcacg gtgacgacca
     1321 ggaggaacgc cgcttcgacg ccaaaggcga ttttggtgat gcggaagcag cggcgcaggt
     1381 tgcgggcgcc gtagttctgg ccgacgaagg tcgtcgtcgc ctggctgaag ccgttgacga
     1441 aacagtagat attgccttcg atgatgaagg ccgcagccga cgctgccatg gcttccgtcc
     1501 ccaggctgtt cagggccgac tggatgagga cgttggacaa ggcaaagacc atgccctgga
     1561 tgccggcagg caggccgatg cggatgatgt gatgcagttc ttcccgggaa aagcgcatgt
     1621 gatggcgttc caggcggatg cggtccgtcg tccagcacag caggaccagg aggatgagcg
     1681 aattgacggc aaaggcgatg accgtcgccc agacgacgcc ggccaggccg aaatcaaaga
     1741 ccaggaccga cagcaggtcg aggacgatat tgacgatatt ggctgcgacc aggctgtaca
     1801 gcggcgtctt gccatcgccg cagctgcgga agatggccgc ctcgaagtta tacagcgaca
     1861 aaaaaggcag gcccagcagg aagatgcgca gatactgttc ggccatatcg tagacgtctg
     1921 gcggcacttc caggacccag aaggccggat agacgatgag ttcgccgatg acggccagca
     1981 aggcccccag tccaaaagac atcaaaatag ccgtctggac cgtccgttcc actttatcgt
     2041 cgcggcgggc accgagatac tgggcaatgg tgacattagc ccccagggaa atccccatga
     2101 gcagcatgac caggatgccg atgacaggca tattgttgcc gacagcagcc atggcgctct
     2161 tgccgacaaa acgtcccaaa ataaagacgt cggcactgtt gaagagctgt tcaaataagc
     2221 ccatgaggcc gatcggcatg gcaaaaaaga acaacttgtc ggcaatagag ccgtgcagca
     2281 tatccatgcc gcggcggccc ttattttgtg tattttccat aattatgatt acctctcgtt
     2341 ctcgtgaatg atgctttctt cccttagtat agcataaaag agcttgccgc acatgcgaca
     2401 agccctttta agtggttagt ggctagtagt tagtggttag cggatggtta aaaatttgaa
     2461 accatcttct acaaactccg tactacaaac
//
LOCUS       DAWABZ010000199         2407 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_9067_length_2407_cov_3.425170, whole genome shotgun sequence.
ACCESSION   DAWABZ010000199 DAWABZ010000000
VERSION     DAWABZ010000199.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9067_length_2407_cov_3.425170"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          564 a          630 c          671 g          542 t
ORIGIN      
        1 cctcgtaagg ggcgccctgt tgacttttct acttcttgcg atataataag gaatgtagtt
       61 ggaattaaaa ctaattaaaa ggggaatcgc catggacgaa gaagcgttgc atattttact
      121 gttgttaggg tttcaccggt cgaaccggga aatcggccgc cgcatcaagc gggaagggct
      181 catgcccggt cagcctaaga tactggaata tctgtggttc catgacggcg ccagccaaaa
      241 ggatatcagc cgcgagtgca tcatcgacaa gtcgacggtg accagcctgc tgaaacgcat
      301 ggaaaagctg gacctcatcc gcaaggaaac gcggcccgaa gaccagcggg gcgtggccat
      361 cttcctgacg gaaacgggct gggaaaaggc caagcggatc cgtgaggtca tttatggcat
      421 cgacgatgaa atgtggcagg gcatcgagcc ggaagcacgc cagcagttca tggcgacgtt
      481 tcatcgcatt atggagaatg aaaagaagtg gggcgattga gtatgcgtca tatgcatcat
      541 ttgaaaatac gggtcagtgt atttctttgc agtatcttga tgacggcctt cggcatcgac
      601 ttgattactg tcgccagcct ggggacgtcg cccatttcca gcgtgcccct cgtcgtcagc
      661 ctctatacgc cgtggacctt cggccagctg acctttgtcc tcaacatggc cttcatcgtc
      721 ttgcagattg ccctgctgcg cgccagttcc ggcccgtaga gctgctgcag attgccatta
      781 acttcgtctt cagcgcagcc atcgactttt tcatgcacgc catggcagga ttacagggac
      841 tgaactggcc ttttgccgca gttctcttag tggccggctg tgcgaccctg gccttcggca
      901 tctgcatcga agtggcgtcg caggtcctga tggtgcctgg cgacggggcc gtcaaggcca
      961 ttgcccagac ggggaaatgg tggatgggct cgacgaaagt cgccttcgat tcgactatgg
     1021 tcaccattgc cgtcatcttg tcgacccttt acggcggcct ggggaccctg actggccttg
     1081 gcgtcggcac gctagtgtcg gccatgctcg tcgggcgctt cgtcaatttc ttcaatcgcc
     1141 gcgtctttct catcgaacgc ctggcccggc tgtccttgga tgatgaaacc aaggcggccg
     1201 gagctttgtc caaagaaatc cagtcctgat tcagggactg gattttattc tttttcgggc
     1261 atataattat agttaataag ctaatcaatg tgttcggata cggttacgct ataatagaac
     1321 gtaacctctt gattcaccta tgtagttagt tagctaatta taatagagag aaaggaatga
     1381 ttaccatgaa gggacaaaca gtgcatctgc caaagcgggc atggcgcctg ggcctctttt
     1441 tcatcggcat cctcctggca ggcggtgtcc tgctcatgta taagggaaat gatgccgtgg
     1501 cccagggtat cgccaataag gacgggatat tgacggcaga gcaggtcaag gtttcttttc
     1561 agtccgtaag tggcaagatg gtgaacgaag ctgtccagga atcacaggag gtcaaggccg
     1621 gcgacatcct catgcagctc gacagtaccg acgttgactt ggatattgcc aagacccagg
     1681 ctcagatagc tcagctcgac gcccagatcc agtcgatgga agggactatc gccatcggct
     1741 ttgtctagac cgatacgtcg caggaagaaa gccggcgcca gatcgaccag cagcgagcag
     1801 ccatcgattc ggcgacggca acgctggtca atgctcagcg tgattatgac cgtaaatcgt
     1861 cgctgtatcg ccagggagcc attgtcaagg ctgattttga cgattcccag gcggctttgt
     1921 ccgtagccca ggccaatatg aaccagcagc aacaggcctt atcgaaatta ttgggcggtg
     1981 ccgtcgatac gggagacagt gattccctcc agctgccgca gatcgaagaa cagcggcaga
     2041 ccattgccaa caagcgtcac gaccgggaat cgctggtcca gcagcgcaat cagctccagg
     2101 tccggctcca gcagctggaa gtccagaaag accgcctgac cctgcgggcg ccggaagacg
     2161 ggaaagtcct caagctcctg gccaagcagg gggaaatcgt ccaggccgac acgccggtca
     2221 tcctcttgga aagtaagcgc cagtattacg atatctatgt cagtgaaaag caggccgtcc
     2281 atctccatga aggccaggcc ctggccggtg tcgccgtatc ctatctgtga ttttgcatca
     2341 gaatgacagc agtttacaac tttttgaaca aataaatatc gcgtaaccac actttgaagt
     2401 ataatga
//
LOCUS       DAWABZ010000200         2397 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_9107_length_2397_cov_2.285653, whole genome shotgun sequence.
ACCESSION   DAWABZ010000200 DAWABZ010000000
VERSION     DAWABZ010000200.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9107_length_2397_cov_2.285653"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          586 a          704 c          672 g          435 t
ORIGIN      
        1 aacatcatgg tcctgcggga cggcagcgtg aaggtggccg atttcggcat cgcccgggct
       61 gtgaattccg tcgaaaacag cgaccgtacc gtcctcggct cagtccatta tttttcaccg
      121 gaacaggcca gtggcggcca tatcgacgaa cggacggata tttattccct aggcgtcgtc
      181 atgtatgaaa tgatgactgg cgtcgtccct ttccagggcg atacggccat cagtatcgcc
      241 ttgaagcacg tccaggacga tattcccttg ccgacgaagt acaaccgccg tatcccgcgg
      301 ctcgtcgaac aggtcatcct caaggccatg gccaagaacc cggacgaccg cttccagtcc
      361 atcggcgaga tgatgtcgga actgcgcctg tcccagggtt tcatcaatac caacggcgct
      421 cggccgatca tcagcaaggg tccgtataac ttcggcacgc agaaattaca gcctatcaag
      481 gaaaaggacc tgcccgaaga agaaccgaaa gaaaagaaga agaacccctt cacgcggttc
      541 atggattcta tcagcaatca ttcgcagaaa actatcatca tcggtatgct cggcgtcttc
      601 ctcgtcgcct ttgtctgggc cttcttttcc tttgggaatt tttggagtac cgaagacatt
      661 accgtaccgg atgtgacggg taaacaggtc gaaatcgcca agaagatact gaaagataag
      721 aaactcgacg tttccgtcaa ggaagtggaa agcgacgaag tgccagtcgg cgaagtcatt
      781 tcccagacac cgtctggcgg agccgtcgtc aaggccaacc ggaccattta cctgaccgtc
      841 agcaagggga ataaaggtga agaagtcctc ataccggacc tgcggagcct gaccctcgat
      901 gaggccgaaa agaaactgaa ggaaatcggc ctgtctgtgg gcaaggtgcg ctacaaagag
      961 agcgctcagt atgccgacgg gaccatcatc gaccaggacc cggaatcgcc gaagaaagtc
     1021 gaaaagggga cgaaagtcga cctcgtcgtc tgccgtaaga gcgaagccga aaaagaagca
     1081 gaagcggcca agaatgaagc ccctaatacg agcggcatga gcctcgatgc ggccgtgaag
     1141 gccctggaaa gcgccggcta ttccgtcggc agcgtgtcca acctcgattc gtcgaaggac
     1201 agctctcagg ccgtcgtcac gggccagacg tcggaagacg gcaaggtcgt caacttgtcc
     1261 gtcgaatatc cgtcgtccca gagcagccag agctcgccgt cgagcagctc cagcggctcc
     1321 gatggcagtg ccaataacca ggaaccggcc gggacgcctc attacgggac ggtcaatatc
     1381 agcgtaccgt ctggcgcttc cagccagcat gtccagatcg tcgtatccga cgacaatggc
     1441 tcgcgcgtcg tctatgaccg cagccaggcc ggcggcgaca acatctctaa gagcgtcagc
     1501 ggcacgggca agacgagagt caaagtatac atcaacaatt cgttagtaca ggaccagtat
     1561 atctaagggg aggaacatga caaaaggaca cgtcatcaag aactataacg gctattatta
     1621 cgtcgatgtc ggccgggaag ggctcatcga gtgccgacgc cgcggcaaat tgctgaaagc
     1681 gaagatcctc gtcggcgacg agctggaaat caccgagctg ggccaggaca agggcgtcat
     1741 cgaagccctg ctgccccggc gcaaccagat acgccggccg gccgtggcca atatcgacca
     1801 gctcctggtc atcatggcgg ccaagtcgcc ggatccgaac cagttcctcg tcgataagat
     1861 gctcatgacc tgtgaatacg gcggcatcca tccgacgctg tgcttcaata agtgcgacct
     1921 ggaccgggag acggccgaat cctacaaggc cttttatgaa cgctgcggct atgacgtcta
     1981 tctcgtgtcg gccaagaccg gcgaaggcct cgataccctg cgtaatctgc tgccccaccg
     2041 catgacggcc ttttccggcc cgtcgggcgt cggcaagtcg agcctgctgt cccagatcct
     2101 gggccgggaa gacttgtccg tcggcgccgt cagcgaaaag atcaagcgcg gccgccatac
     2161 gacacgccat tcggaaatca tgcgcctcga cagcgatacg tatgtcgtcg atacgccggg
     2221 attcagctct ctcgacttcg agcacctcga cccgaaggaa gtcctgcgcc tcttcccgga
     2281 catgctgccc tacagcggcg gctgcaagtt ctcgtcgtgt ctccatctgt ccgaaccgga
     2341 atgtacggtc aaacaggccg tcgctgacgg ccatatccag aaagaacggt atgacac
//
LOCUS       DAWABZ010000201         2378 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_9168_length_2378_cov_1.964701, whole genome shotgun sequence.
ACCESSION   DAWABZ010000201 DAWABZ010000000
VERSION     DAWABZ010000201.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9168_length_2378_cov_1.964701"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1186..1195
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1865..1874
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          567 a          607 c          546 g          638 t
ORIGIN      
        1 ctctttgctc gtctgtacat actcagccgc atcattcaga caattcagcc gatctagtgg
       61 cttgccatga taaaacaagg tagcatcgaa ggaggagagc aagtcatcga tcatggacaa
      121 ataattccta aaaatggata gggagacatt cagctcatcg acgggatttt cctggggacc
      181 gccgtacttc ttgacagcgg ccatcatatc atttttaatg ccgatataat cgacgaccaa
      241 gcctttgtct ttcccatcaa agacgcggtt gacccgggaa atggtttgaa tcagcgtatg
      301 tttctgaagc ggtttgtcaa tgtacattac ggtaagggaa ggaacgtcga atcccgtaat
      361 ccacatatcg acgacgatgg cgattttgaa attcgagtca ttatttttga actgcttatc
      421 gagcatattc cgatactctt tcgttccgca ggtatcatac agttcttttt catcattctg
      481 cccctgcgtg gctaccagat tgattttagg aagtgccacc agctgatcca actgttcccg
      541 tgtcaatttt gattcgtctt ccgccttgcg cgggatacgc cagtccggcc ggattgtttc
      601 tatctctttc aaaagctgga acgcattttt cctgtccgaa caaacaatca tggccttttg
      661 cacgacatcc ggcttttctg cacaaagcgc ttcataatga gcgaccatat cagcggccag
      721 tttttttatc cgttccggat gccccagtat cgccgacatg gtactcatgg cgcgtttgct
      781 ggcctctacc tgctctggat tggccccttc ttccacgcat ttttcataat atttttcgat
      841 ttctttcgcc tgctcatcgg aaagaatgac ccgcgccaga cgaggttcat aggcaatgcg
      901 gacggtaatc ccatcgtcac tggattcctt catggtataa ctatcgacga ccggaccaaa
      961 aacggctacc gtttcatcaa tcggcgttcc tgtaaatccg cagtacgtcg cattggggaa
     1021 gctgtcccgg agatacttgg caaagccgaa ggtcgtatac acgccagacg catttttctt
     1081 cagtttcgag ccaatattta cctgcgtccg gtgtgcctca tcggaaatac agatgatatt
     1141 gcttcggtcc gaaagaagtc ccgtatcttc acagaatttt tgtatnnnnn nnnnnccgcc
     1201 gctaggcttg tctcgaagcg tcttttctaa atcatgacgg ctttcgatgc tcctgacatc
     1261 ggtctgatgg agatagcgct tagccgtgac aaataattcg gaagtctgtg tgtccaggtc
     1321 gtcccggtct tccagcagga ttatggtcgg actcctgaaa gtttcggcat cgcgaagcat
     1381 aatcagccgg gataaaaaca acatggcata ggtcttgcca cagcccgtcg cgccaaaata
     1441 ggtcccgccc ttgccgtcac cagcaggccg catatgttgt ttgatatggg ccagcatttt
     1501 ttctgctgca aaaaattgag gataccggca gactatgact tcatccttgg cgctgtcatc
     1561 cgggtagaag acaaagtccc gcagtatttg gagtatacgg tctttggcaa aagccccttc
     1621 tatcatcgtc atgagcgaac tgatgccatg agcgactgta tcggtatcat tggccttatt
     1681 ccaggcataa taaaaccggt agggcgtgaa gatactgcct agtctcgtat tggccccatc
     1741 acagatgacc gataaaaagc aatacttcaa cagattcggg atatcccgct ggtaacggat
     1801 ggtgatttgc tcccaggcgt catgaatcgt tgtgtcttct tcaatggccg tcttaaattc
     1861 ccagnnnnnn nnnntcccag atgctgacgg gaatcccgtt gatgaacaaa agcaggtccg
     1921 gccggcgacg ccgtttcccc tgcacggaat actggttcac gacgcggaaa atattatgtt
     1981 ccggatgttc aaaatcaatg aaatcaatat ggacgggcaa ttcacctaaa cgatcacgga
     2041 ccaggtcaaa gccttccacg accagccaga aggcctgccg gttggccgca tagagcggag
     2101 ccgaaggaat caagctcaac atgttcacga ttttcttcat ttctacgtcg ctgaggtgct
     2161 tgtcctgata cttgcgagtt agaaaggcac gaaggtcctc ttccagaagg accgtatcca
     2221 gttcgcggtg aatggcttcg ccgcaagtgt attcgtaccc ctgttgtcgg aagagttcga
     2281 taatagcctg ttccagttca gcttctgtaa atttcccttt cgtgaaaaaa tagtccatcg
     2341 tctcatcccc tttttagacc cggccttcgt ccagcgag
//
LOCUS       DAWABZ010000202         2378 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_9165_length_2378_cov_3.436505, whole genome shotgun sequence.
ACCESSION   DAWABZ010000202 DAWABZ010000000
VERSION     DAWABZ010000202.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9165_length_2378_cov_3.436505"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          472 a          697 c          628 g          581 t
ORIGIN      
        1 aggctatcct gatttcagga ggaaacttaa acagcaacct tctcaagaat ctcccacttc
       61 tacaagcggg agaggttcaa atttattttt catatctgtc atgtgcggac agcgtcacga
      121 ttccgacagg gcttcccatt tttcataggc ttcatcgagc tgagcctggg ccgcttcata
      181 ttctcgcgtc aattccatca tggcgtcggc atcggtctga ttctgaggca tgttcatctg
      241 cacttcgtac atcttgatgg tcgcttccag ttcggcaatc ttcatctcga ttttttccag
      301 cttggctgcc tgaccatacg ccggagcctt aaccggtgcc ttcacggcct gagcggctgg
      361 tggttccgcc ttcttggctg ccggagcggt ttctttttca tgagccggcg aggaaccttg
      421 cccttcggct gccgcctttt ctgccgcttc ctgggcttcg cggacctttt cccggtaata
      481 actgtaattg ccgacgtatt ccgtgagctg cttattttcc aggaccagcg tccgctgggt
      541 gattttgtcg aggaaatagc ggtcatggga tacgaccaga taggtcccgc cgaattccag
      601 caaggcctgt tccaggactt cccgcgtcgg gatgtcgaga tggttcgtcg gttcgtcgag
      661 gatgaggaaa ttcggcccct gcaggaagag cttcaacagg gccagccggg cctgttcgcc
      721 accgctgagg ctgtcgatga ccttgaagac atcgtcgccg cggaacatga agcgccccag
      781 gacggaccgg gcttcttctt cgccataact gaaatcgttc atgatttcat cgaggaccgt
      841 ccaagctccg tgcaggtccg tgtgttcctg ggcaaaatag ccgatatgga cgcggctgcc
      901 caaggtgata tgaccgtctg tcggttcctt ttcgcccatg atcatgcgga tgagcgtcgt
      961 cttccccgtc ccgttagggc cgatgaggga cgctgcttcg ccgcggcgca ggaggagcga
     1021 cagatggtcg aacaactggc ggtcggcaaa accgccgctg accttgtcca ggaccaggac
     1081 tttctggccg cactcttcga caggcttgaa attgaaatgc agcgattcat cttccggctg
     1141 ccattcgatg cgttccagcc ggtccagctg ggactgacgg ccgcgggcct gcttggcctt
     1201 gatgccggcc ttgtacttgc ggatatattc ttctgtttcg tggatgtgct cctgctgctt
     1261 cttataggct ttttccaggg ctttctgctg ctgggcatgc tgctcgacgt agcgcgaata
     1321 atttcccttg tacttagtca ccttgccgtg ggacagttcg aggatgcccg tcgccacgcg
     1381 gtcgaggaaa tagcggtcat gggatacgat gaggatgccg ccgccataag acaagagata
     1441 gctttccagc cattccagca tgtccacgtc gagatggttc gtcggttcgt cgaggaacaa
     1501 atagtccggc cggcggacga gagccttggc caggttgatg cgcgtcttct gaccgccgga
     1561 aaagctctgg accggccgat ccatatcggc ttctgaaaag cccaggccct ggacgatttt
     1621 ccgcgacatc gactcgtatt catagccgcc gagccactcg aaccgttcct gcatgcgggc
     1681 atagcggttc atcaagcctt catcgtcggg atgggcctgc atgtcctttt cgacagcctt
     1741 caaatccttt tccagcttca tgacatcctg ccaggcctcg gtaatcgtct ggcgcaacgt
     1801 tacgtcgtcg gcgtaagtga tatcctgctg caaatagccg atagattcgc cttcggaaac
     1861 gacaaacgcc ccgttatcat actcctcttc gcccatgagg cacttgagca gcgtcgactt
     1921 cccggcccca ttggccccga tgaggccgat ccgttcgccc cggcggattt caaaactgac
     1981 atcatggaat accgtatgta ccccaaaaga cttactcaat ttctgtactt taatactacc
     2041 catctatatg atacctgccc tccctggcat actgacgaca aaacaaaaaa agagccgccc
     2101 cggatcccca tcttgggcgg ctgcctattt tcacatatct attataccgt caatcctcaa
     2161 gcatcgtcaa gacggcgatg ataataagac gccggtcgca ggcggcaggc cgcaggccgc
     2221 aaaaaaatac acggcctgcg gccttgtgcg taggggccgg ccaaagggcg gcccgctgtg
     2281 atatttcgcc acagcctaac cgagtatgat ggcggagctt taccaacaac catctacggc
     2341 cttttcccgg attcagatgc ggctttgccg cctcaaat
//
LOCUS       DAWABZ010000203         2325 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_9376_length_2325_cov_2.402643, whole genome shotgun sequence.
ACCESSION   DAWABZ010000203 DAWABZ010000000
VERSION     DAWABZ010000203.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9376_length_2325_cov_2.402643"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          523 a          584 c          547 g          671 t
ORIGIN      
        1 aggcggttcc tggccgtgat agctacattc cccggaaaaa gcgatgatag ccttataagg
       61 gctgtgccgg tcggccagac acttggtaat ggcatagtac gtttcaatgc aacggggaat
      121 actggccgtc accaccatgg cgcgagattg gccgcccagt ttcttcctgg caatgatctg
      181 ttcgtggaaa tggtctacca tcatcgccgc tttcttggca atgacgtcgg gattcccttc
      241 gacaaaggag cgcagtttct tctgagcccg cttcttgtca aacatagggt cgtcttccac
      301 ctttttggca atcttatacc aactgtcgat ggtcacgtaa ttcttcagca catccaggat
      361 gaagccttcc tgaatggctt gtttcatggt atagacatgg aagggccggt gtttgatttg
      421 gccgtcttct tcataaggcg tgccaaacac ctcttccgtc ttgtttttag gcgtagccgt
      481 aaaggcgaag taactggctg tttttaccag tttccgtccc tctaccatgg cgttgatctt
      541 gtcttcatta tccagttcac tgtccgaagc cagtccggaa agggccagat tcatattggc
      601 tgcattccgc ccactctgtc ccgaatgggc ttcgtcgatg atgatggcaa aggtatggtt
      661 tatatgttcc tggccgattt cttgagaaat ataagggaac ttctctaccg tggtcacaat
      721 gatccgcttg ccgtcctgga tggccttttt caggtcgccc gagtgctggg cccatacgac
      781 ggtattcttt acctgcatga actgtttaat cgtatcccgg atctgcttgt ccagaatccg
      841 ccggtccgtc acgacaatga ccgaatcaat cataggccgt ccatcccgtt ccagtccgat
      901 cagctgatgg gccagccagg caatggaatt ggatttgccc gacccggcgc tgtgctggat
      961 gagatagcgt ttacccacac cgtactgcct gacatcggcc aggagttttt ccacacagtc
     1021 caactggtga tagcgcggcc agatttgttt gaccgatttc ttccgggttt ccggattttc
     1081 ctcaacgacg acctggacgt agttctcgat gatacgggac agtttcgtct tcgtcaggat
     1141 atctttccac aagtagtccg tcatgatgcc ctcggggttg ggcggattcc cggccccatc
     1201 gtgatatcct ttgttgaaag gcaggaacca gctggccttc ccgcaaagtt tcgtacagaa
     1261 ctggaccgtc tgatcatcta cggcaaaatg tacgatacag cgcttgaagg aaaacagcag
     1321 ttccctggga ttgcggtctt tcttatactg ttctacggcg tcggctgtcg tctgcccggt
     1381 gaaatggttc ttcagctcta tggtaatgac gggcaggccg ttgatgaaca gacagaggtc
     1441 caaagccaat tttgacgcgt cgatagcata gcgcagctgg cgggtaacac tgaaaatgtt
     1501 cttgtcaaat agccgtttcg cctgctcatt gttttccgtc ggcgtgaaat agaacaggat
     1561 caggtcggcc ggataggctt tgatgccatt gcgcaggacg tcgatgatac cgcgccgggt
     1621 gatttctcca gaaaggcggt tcaaaaactg ccgcttcttc tggtcactct ggttgacgcc
     1681 gagctttgcc atctctttgg gttgagtatc attcaaaaag cgaaagagcc gggtttcatc
     1741 gatggcatat tctttattgt aatcttcatt cgtcccctgc tggtagccat cctgatccac
     1801 cagccagttc acgatgagcg tttcaaaacc cttttccgtt ttatccgtaa aaaccatcgt
     1861 cagtcctcct ctccagggat tcctgtttct tcgtcattgc cttccccatc atcagccgtc
     1921 aagtcgtcca cgtgttcgta agcaggaaca gggatattcc ggacatcaat tttgccggtg
     1981 accacgtcgg agatgagggt ggattttagt tcttgcagtt gcttaatttt tttctttaat
     2041 aaagatattg tttgattaat taaaatttcg ttcttatgca cttgagctgt aatagctaac
     2101 tgctcttcat actttggaac taatatttta attctcataa gtttagaagt gtcaattgcc
     2161 ggatacgcga taccaataga ttcacgaata acctcatcgc aaaaacgttt ggatttaaaa
     2221 taatactcca aatatttgct atccacacca tgaggactta aaaccgcaaa tccagtagat
     2281 acaattacat ccttatcatc cgttattcgc gtaattgcct taaga
//
LOCUS       DAWABZ010000204         2297 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_9487_length_2297_cov_3.211864, whole genome shotgun sequence.
ACCESSION   DAWABZ010000204 DAWABZ010000000
VERSION     DAWABZ010000204.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9487_length_2297_cov_3.211864"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          459 a          621 c          666 g          551 t
ORIGIN      
        1 gtggggcgcc cgctctggga tatgtacgat ttcccgaccc gtttgctaga agaatcatcc
       61 aaattcctaa caggtctccc acgtggcccc tacaaactat agagcatagg aaccccgcga
      121 gcggtccttc ttcttcccag gggaagcctt aatgatggaa caggcgcagg ccggtcatgg
      181 ccatggcgat gttgtattta tcgcacgttt cgatgacatt gtcgtcgcgg atggagccgc
      241 cggcctgggc gatgtagtcg acgccgctgc gatgggcccg ttcaatgttg tcgccaaagg
      301 ggaagaaggc atcgctgccc agggctacgc cgtggagctg ggcgatccag gctttctttt
      361 cttcccgtgt gagcggttcc ggtttttccg tgaacaagtc ctgccagatg ccgtcggcca
      421 ggacgtcttc gtagtcatcg gagatgtaga cgtcgatagc gttgtcgcgg ttcggacggc
      481 ggacatcgtc gcggaaaggc aggttcagga ctttcgggtt ctggcggagc caccagatat
      541 cggccttgtt gccagccagg cgcgtgcagt ggacgcggga ctgctggcca gcgccgacgc
      601 cgatggtcat gccgtctttg acgtagcaga ccgaattgga ctgggtatat ttcaaagtga
      661 tgagggcgat catcaaatcg cgctgagctg ctgccgggat attcttgttc ttcgtcggga
      721 catttttgaa gaggtccgtc gtaatcttgg cttcattgcg ttcctgttca aaagtgacgc
      781 catagacttc cttcgattcg acgaggcgcg gcttgtagtc cgggtttatc tggaggacgc
      841 agtaattccc cttcttcttg gtcttcaaaa tagccaaggc ttcgtcgctg tagcccggag
      901 cgatgacgcc gtccgagact tcccgcttga ggagcgttgc cgtttcgaca tcgcagacgt
      961 cggacagagc gacgaaatcg ccgtacgagc tcatgcggtc agcaccgcgg gcgcaggcat
     1021 aggccgaagc caggggggac aatttcaaat cgtcgaccca gtatatcttt ttcagggtat
     1081 cactcatgtc cgtagcgacg gcggccccag ccgggctgac gtgcttgaag gaagccgctg
     1141 ccggaaggcc tgtcgcttct ttcaattctt tgaccagctg atagctgttg aaggcgtcga
     1201 gcaaattaat ataaccggga cggccgttga ggaccgtaaa gggcaggtcg ctgccatctt
     1261 tcatataaac ccgggccgac gactggttgg gattgcagcc gtattttaat tgcagttctt
     1321 tcatggtgat tcctcctgaa tagttaagca aaaataaaaa ataccgacac ttctggaacg
     1381 tttcgcccag acggtcggct cgtacgaatc atgctccccg tggtaactcc acttccgtca
     1441 gtcacatgtt tcagatttat tataccgtcc gcagacggga gatgtcaaga aaaaggaccg
     1501 cccttcggac ggtccctttt tctgtagggg cgccacgtgg ggcgtccgct ccaggatgat
     1561 gtgatttcca tttcccattc ggtttactgg gagaaacatc caaattccga acggggcgcc
     1621 cacgtgcgcc ccctacagtg ccactatcca ctaacccgcc tgttgttcaa agatgtcctg
     1681 ggccaggccc atgggttcat cgcggaagag gcgttcatcc atggtcttca gattttcggc
     1741 gatgtgcggt acgaattcca tctggtcgag gatgtccttt tgcagatcca cgcccggtgc
     1801 gatttccgtg aggactaagc ccttatcggt gaggacgaag acacagcgtt cggtgatgaa
     1861 catgacctgc tggttgtgtt tatgggcata ttcagcactg aaggtaatct ggccgacctt
     1921 atcgatgaat ttctggacag ccccttcatg ggtgatgacc agtttgccgt cccggatttc
     1981 ttcgcgcagg cccttggccg taaaggtgcc gcagaagacg actttatgcg tattctgggt
     2041 gatgttgatg aagccgccgc agccggcgat gcggtctttg aacttgctga cgttgacgtc
     2101 gcctttggcg ttacattctg ccatgcccag gaaagccata tccaagccac cgccatcgta
     2161 aaagtcgaac tgatacggat ggtcgacgat ggcatcggcg ttggccgaac cgccaaagtc
     2221 cgcgccgctg gccggtacgc cgccgatagg cccggcttcg acggtcaggg tcagctggtc
     2281 gccgatacct tcttcgt
//
LOCUS       DAWABZ010000205         2228 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_9789_length_2228_cov_3.268293, whole genome shotgun sequence.
ACCESSION   DAWABZ010000205 DAWABZ010000000
VERSION     DAWABZ010000205.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9789_length_2228_cov_3.268293"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          522 a          580 c          610 g          516 t
ORIGIN      
        1 cggcactatc ttccgtggcg aagccaacgc acgataaacc atcgggaggg atagtcatga
       61 atctgacctt gttagggacg gggaacgcaa cggtgaccca ttgttataat acttgtttcg
      121 tcctccatga aagcggtcaa tatctgctcg tcgatggcgg cggcgggaac ggactcctgt
      181 gtcagctgga aaaggcccat ttgcgctggc aggatatgcg gacggtcttc gtcacccata
      241 aacacatgga tcatcttctg ggcatcctct ggcttatgcg catgatgtgt cagtcctggc
      301 ggcgcgggac ctatgagggc gaagccgtta tctacggtca tgatgaagtc atttccctgc
      361 tgcgcacgat gggaaaggct ctgctgtcct cgaaagacag tgctttcctc gatgaccgcc
      421 ttcatcttgt gacggtgacc gatggggaga cgcgtcagct catgggtcat gacgtaattt
      481 ttttcgatat tcgttcgacc aaggccaaac aattcggctt ttctttagaa ctcgccgacg
      541 gcagactgac ctgctgtggt gatgagccgt atcagccctg ggaagaagcc tatgtccggg
      601 atagtaaatg gctccttcat gaagctttct gcctcgatgc cgaagcggat atcttccatc
      661 cctatgagaa acaccattcg accgtcaaag acgcctgcca aacgtcagcc cgcctgggcg
      721 tacagaatct gatcctctac catactgaag acaaggacct ggcccatcgc cgccagcgtt
      781 accatgccga aggaagccgg tattatcagg gaaacctgta tatcccggac gatttggacg
      841 tcatcgcttt atagtttgta gtacggattt gtaggaggcg cacgtgggca atccgttcgg
      901 gatttggatg gtttttctag caaactggtc ggggaatcgt acatatcccg gagcgggcgc
      961 cctacttggg cgcccctaca gaaaagggct tatcgtcggc gacaagccct tttcgttgta
     1021 caatgtatcc gattctgtta gaatagacgt aaggccatgg cctcgaaaga atttggcagc
     1081 ttgggaaaat gttgcagcaa aaattagcag aagttgaaga agatcattaa gggatagagt
     1141 aaggagacag ggtaaaatga tacgttttaa caatgactat aatcatggcg ctcatgccag
     1201 cgtcttgcag gctttgaatc agacgaacga tacgcagtac ggcggctatg gtgtcgatga
     1261 ctggtgtgac aaggcagaaa agctgatttt ggaattggcc cagtgcccgc aagggaaggt
     1321 ttactttttc ccgggagcga cgcaggccaa tttcgtcacc atttcagccc tccttggttc
     1381 ctgtgacagc gtcatctgtg ccgacacggg ccatatcaac tgtcatgaat gtgcgtccat
     1441 tgaaaataca ggccacaaaa tcgtcgccct gccccatcat gacggcaaga ttacggcgga
     1501 ccagattgaa ggggaagtgc aggcttacta taattccggc gaagccgaat acctgacgcg
     1561 gccgtcgctg gtctatatct cttttacgac ggaatacggc acgctctata ccaaggctga
     1621 actgacggct atttcggaag tctgccgcaa gtatcacatg tacctcttcg tcgatggcgc
     1681 ccgcatgggt tatggcctgg gggctgacgg gaatgacctg accctggctg atttcggccg
     1741 cctggccgac gtcttttata tcggcggtac gaaatgcggg gccttctttg gcgaagccct
     1801 cgtcttgacc gacgccggga tagaccgccg cttcaaagct cacatgaaac agttcggcgc
     1861 cgtcctggcc aaaggctgga ttttagggct ccagttctat accctgctcc acgacgggac
     1921 gtattttgcc attacgaaac gggccgacga atacgccgtc cgtatccgcc aggccttccg
     1981 gcagaaaggg atagaagaac tcgtcgtcag cccgacgaac cagcagttca tcatcctttc
     2041 gagagctcag gcccaggcct tggaaagcaa atacatcttt gaagccatgg gacctgttga
     2101 cgatgaccac gtctgcgtcc gcgtctgcac gtcatggagc acgaaagccg aagaagtcga
     2161 ccagctcgta gccgatattt taaacctgta ggggcttgca cgtggcaagc ccgtccctgc
     2221 atatgagc
//
LOCUS       DAWABZ010000206         2167 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_10053_length_2167_cov_3.075758, whole genome shotgun sequence.
ACCESSION   DAWABZ010000206 DAWABZ010000000
VERSION     DAWABZ010000206.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10053_length_2167_cov_3.075758"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          441 a          584 c          619 g          523 t
ORIGIN      
        1 ggcccgcata ctgccgacga ctttggccag tttctggcct ttatggactt tatcgccatc
       61 tttgacgaag aactcgaacc agaccttgtc gtccagcaga gtaaagggcc gggcaaagac
      121 atcgaggccg gcgatgatgc cgtcctgttt gcacaggagt tccgcttcac cctggcagtc
      181 ttccggcaaa atggcattgg tcgtcacgtc ttcaccggtg atgtcttcct gcagggcctg
      241 taaaatcaaa ggatcagcct gcaatttcat ggttacgctg ttcatcacat tcacgctccc
      301 tttccatttc acctaagaca tgacggcggt attcttcagc cagtgtatct tcatgactgc
      361 cgatgaaggc ttctgacggc gcacaggctt gggcaaagtc ggcggcactg actacctgct
      421 ggggctgctt ttctgtttcc acgatgtgct gggctgcgcg cttgccgaag accaggcttt
      481 ccaggagaga attgctggcc aggcggttgc ggccgtggac gccgttgcag ctcgtttcgc
      541 cgacggcata gagatggtcc atcgtcgtct ggctgtactt gtcgacgtgg ataccgccca
      601 tgaagtagtg ctgggccggc gtgaccggga cccagtcctt cgtcgcatcg atgccgtctt
      661 ccaggcagcg ttcataaatg tgcgggaaat gatgcttaat gacgtcttcg ccgaggacgg
      721 tcatgtcgag ccagacgaag gggcggtcgt ccttcttcat ctgtttgcgg atttcggccg
      781 tcatcaaatc gcggggcagg acttcgttgg caaaacgctg accgtctttg ccgcgcagct
      841 tggccccttc accgcggact gattcagaaa taaggaagct ccggcccggc tttttggaat
      901 agagcgacgt cggatggatc tggacgtagt cgatgtgttc cagttcgacg ccatggcgca
      961 tggcaatggc cagggcatcg ccagtgagat gcgggaaatt cgtcgaatgg tcatacaagc
     1021 cgccgacacc gccactggcc agaatcgttt catcggcctg gatagctcgg taacggccct
     1081 ggctgtcgcg ggcgatgatg cccaggcagc ggttcccatc ggtgacgatg tcgaccatag
     1141 tcgtaaaatc gcggatagtc acattgggca gctggcggac agccgctaag agatggctgg
     1201 tgatttcctt gcccgtaata tcttcatgga acaagatacg cggccgcgag tggcagcctt
     1261 cccgcgtata atcgagagaa ccgtcggctt tgcgggcaaa atcgacgccg tagccgatga
     1321 gttcattgat gaccggccgc gacgagcgga ttatgatatc gacggattca cgccggtttt
     1381 catagtggcc ggctttgagt gtatcttcca tgaaactgtc gtaatcgtcc ttgtcgtgaa
     1441 ggacgcagat gcccccttgg gctaaaaaag aatcgctgtt ttctacttca tctttggtaa
     1501 tcatcaggac tcgtttatcc cggggcagat gcagggcggc aaagaggccg ctggccccgg
     1561 taccggcgat gacgatatct gttttttctg tttccattgc tattaccttc ttctatttgg
     1621 ctaattccag catccgttcc agcgaacgga cagcttttcc ggcgacatcc gggtcgacgc
     1681 ggacttcatt cgtttcatcg cgcaggcagt cgcggacttt ttccagcgtg actttcttca
     1741 tgttgatgca atacgcatct tccatggctg gataaaaggt cttgccagga ttgcgcgttt
     1801 ctaattcata aaagacgccg ccttccgtac cgatgatgaa ttccttggca tcggacttgc
     1861 cggcatagtc gatgatgccc gacgtactgc cgacataatc ggccagggcc aggacttctt
     1921 tgcggcattc cggatgggcc aggaacaagg cctggggatg agccgcctgg acctgtttca
     1981 cataagccgg gtcaaccgta tcatggcgcg gacagaagcc gtcgttgagg ataaaatgct
     2041 tgtccgggac ctgcgtcgat acgtaatggc ccagattccg atcaggcgta aagaaaatat
     2101 gcttgttcgg cagtttccgg acgatcttga cggcattcga cgacgtgacg cagacatcgg
     2161 acacagc
//
LOCUS       DAWABZ010000207         2166 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_10063_length_2166_cov_2.487447, whole genome shotgun sequence.
ACCESSION   DAWABZ010000207 DAWABZ010000000
VERSION     DAWABZ010000207.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10063_length_2166_cov_2.487447"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          424 a          570 c          620 g          552 t
ORIGIN      
        1 gtataggcac ccggatgggg atccagcgtc cggatgagcc gttccaatac ggccgcatcc
       61 tgggaccagt cgatgtgggc ttcttccttg gtaatcttgg ccgtatacgt cgcctggctt
      121 tcatcctggg cgacggcgcg gctttcatag ccatccaggt cggtcagggc gtcgagcagg
      181 gtctgggccc cgaggatgga caatttatcg aagagggtcc ccgtcgtttc cttggcatcc
      241 aggggaatag ccgctttttt cagcatcttc cccgtatcca tgccttcgtc gagaagcatg
      301 atggtcacgc cgctttcggc ttcgccgtct ttgatggcgt actggatggg ggcggcgccg
      361 cgatacttcg gcagcagcga gccgtggacg ttgaggcagc cgtatttcgg gatgtccagg
      421 acagctttcg gcaaaatctt accgtaagcg atgacgatga tgacgtccgg cgccaggctg
      481 cggagctgtt cgatgacgtc gtcactgcgc atggaatcag gctggaagac ggggatgccc
      541 agttccaggg ccttttcctt gaccggcgag aaagtcacct tcttgccgcg tccccgctgt
      601 ttatcgggct ggctgtagac ggcagcgata tcatagccgg cctgggccaa tttttccagg
      661 gacgggacgg aaaagtccgg cgtccccatg aatacgatac gtaacttatc cattagtccc
      721 ctactttctg taaatccgtc gccttttcga tgaacagatg gccatagaga tggtcgattt
      781 catgctggaa aatacgggcc aggaagcctt ccggttcata gatgacggtc ttgccgtgac
      841 gtgtcagcga acggaccttg attttggaag cccgttccac tttgccgtaa tatccgggga
      901 tgctcaggca gccttcggta tcgacattgg tttcgtccga tttttccagg atttccggat
      961 tgatgagctc gatgaggccg ttgccgtcgt cgaggacgat gagctgcagc gattcattga
     1021 cctgcggcgc agctaagcct acgccttcag cagcatacat cgtttctgcc atgtcgtcga
     1081 gcaatttctt tatatgttta ttcactttcg taacgggttt cgccgtcgct ttcaggacgg
     1141 gatccccggc tttaataata gtaagtactg ccatgtaact ttcacctcat tgtaatagta
     1201 aaaatgctta tttttttcta ttatagtatg aaatcgccag aaatgctata gattcatcag
     1261 aattgcttga aatagcccat ttctctcctg ccgtaattga caacatttat aaggttatgt
     1321 tacatcgtca gcaatggaag aagtaacact gagggcggtt ccggacgtat cctgaagggc
     1381 gccatggcca tgaagtttct cagaatgctt ctttcatttt ttattttgca ctgtaagggt
     1441 cgccacgtgg ggcgcccgtt cctgcccgat gtgggaatgt actcacatcc cagacgggct
     1501 gaacaggcag gtgcgattcc ccacgattat acgggatcga catcgatgag gacgccggtc
     1561 tgccagcagg ccgggatgcg ctgcatggct tttttgatgg ctgtcaggtc ttttccggtc
     1621 agggacagga ccaggcggta tttattgcgt atcttcttga tgacgtcttc gtaaggaccg
     1681 atgacggcga cttcatcgcc gtggtccccg ttccagctgt tcagggccgc tgcgacgtcg
     1741 ttggccttgc gccagacttg ttcttctgat tcatcctgga ctaagatctt ggccatttcc
     1801 tggaaaggcg gatagcccaa ttctttccgg aactggattt cctggtcata aaaggcccgg
     1861 taatcctggc gggcagcgca ctggatgacg taatggtccg gcgcgtaggt ctgcatgacg
     1921 acgcgccccg ggatatcacc gcggccagcc ctgccagccg cctgggtcag gagctggaag
     1981 gtccgttccc cagcccagta ggccgggata ttgagcaggc tgtcggccga taagatgccg
     2041 acggcgctga cgccggggaa atcgtggcct ttggcgacca tctgagtccc caggaggatg
     2101 tcatattcat gacggcggaa agcttcgatg atacggtcgc cgctgtgctt gcggctggtc
     2161 gtatcc
//
LOCUS       DAWABZ010000208         2129 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_10217_length_2129_cov_3.744937, whole genome shotgun sequence.
ACCESSION   DAWABZ010000208 DAWABZ010000000
VERSION     DAWABZ010000208.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10217_length_2129_cov_3.744937"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          385 a          535 c          685 g          524 t
ORIGIN      
        1 tctgttttct atccaacagt tcataatact catccagctt ttccttgcag gcagccagtt
       61 ttgtgatcac ctcatctttt ttataaatcc gaatcagtta aatacggaca agaccttatt
      121 tcttatctcc atagccctgc ggatgatgct gatgccagtt ccaggcatcg gcaatgattt
      181 gttctacctt actgtgtgtc ggctgccagc ctgtgacgcg gcggatcttt tcggatccgg
      241 cgatgaggat ggccgggtcg ccggcgcggc gcggtgcttc ttcgactttg aagtctttgc
      301 ccgtgacttt cttggcggct tcgatcattt cacggacgct gaagccattc tgagagccta
      361 agttgaagta ctgcgattct cccccttcgg cgaggtactt gaggaccctt acgtgggcgt
      421 ctgcgaggtc tgtgacgtgg atgtagtcac ggatgcaggt gccgtcgggc gtcggatagt
      481 cggtgccgaa aatcttgatg ctgtcccgct ggcccagggc cgtctgcagg atcaggggga
      541 tgaggtgtgt ttccggtgtg tggtcttcac caatggtgcc gcttccatcg gccccggcgg
      601 cattgaagta gcgcagggcc acgtagcgca ggccataggc ccggctgaac tgggccagca
      661 tggtttcgat catgagcttg gtctggccgt agacattggt cggatggtag ggcatgtctt
      721 cggtgatcgg cgtctgttcc ggttcgccgt aaacggcggc cgtcgacgag aagacgaagt
      781 atttgacgcc ggcccggcgt acagcttcca ggaggcagta cgagccgacg acgttgttgt
      841 cgtaataaat tgtcgggttt tccatggatt cgccgacctg gctatgggcg gcgaagtgca
      901 tgacggcgtc gatgttcatg tcttccatga tgtgccgtac ccggtcgatg tcatggatgt
      961 cgacgacgta aaaagggatt tcttccggga cggctgcccg atggccgcgc gaaagattat
     1021 caatgacgac gacgttgtgg ccctgaacca ataaggcttt gaccgtatgg ctgccgatgt
     1081 agccggcgcc gccggtaacg agaatattca ttgagagtct gcctttcgtt atttattgag
     1141 cgggctcccc acgtgggagc tcctacgata atgtttttgc ggattataaa ttatttttcc
     1201 tgcaattttt tcagacggcc gccaaggaat ttcttgtaga cttcgacgcc gggctggttg
     1261 aaggcgtcga cgttggcgta ttctccttcg taagcgatgg acaggcagag catgaacatc
     1321 aattcgccca ggtgatacgg ggtcagggcg ggcaggatgt agttggcgct gggacggccg
     1381 tcaccggcga gggcttcggc gttggagctg cgggccgctt ccaggatctg gctcaaaggc
     1441 aggccgctga aggcagcgag tttcgggtat tcgtcataga gatggggtac cgtgacgtcg
     1501 tcgggccagt tttcgacgga gacgaactgg acgactttgt cctgcgggcc ttcctgatgt
     1561 tcctgggtct gggcgtgcat gtccgtcgtg ccgacggcga cgataggcgt gcggccgtag
     1621 ttgacgacgt tgccctgttt gtccaggcgt ttgcccaggg attcagccag gagctggatg
     1681 taccattcgg acagggattt caggttatcc gcatagggca tgaggacttc caggtcgcgg
     1741 ccgtggttct tgccggccag gaatttgagg acactgttga gcagggccgg gttctgccat
     1801 atatccgggt tctggcaggc gtggtccatg tcgcgggcgc cgtcgaggaa ctgacggatg
     1861 tcaaagcccg tgagggcacc ggcgatgagg ccgacttggg agaggacgct gaaacggccg
     1921 ccgacgccat cgggaacgta gaagatgggc cagcccgatt tcttggccaa gccgtggagc
     1981 aatgtttctt tttcgccgcc gtcgtggggg tccgtaacgg ctaccgtttc cagggcgatg
     2041 ccggcatcgt tcatggcctg tttgaggacc atgtagttgg acatgggttc aatggtcgag
     2101 cccgatttgg aaatgacgac ggccatgac
//
LOCUS       DAWABZ010000209         2124 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_10237_length_2124_cov_2.915418, whole genome shotgun sequence.
ACCESSION   DAWABZ010000209 DAWABZ010000000
VERSION     DAWABZ010000209.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10237_length_2124_cov_2.915418"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          526 a          471 c          523 g          604 t
ORIGIN      
        1 cttcattggt atccttagct atttgagtta cttcttcttc cgtaaatccc atgacatcgg
       61 cataggcatt atcaaggatt gagcagacac ggagattgtt caggccgctg aagatactct
      121 ctttggcaat gcgcaggaca ccggtgagga tggcgaaatt caggaactcg ttttactgtg
      181 accatcaatg agggattgaa tgaaatgagt tttatcgacg aaataataat tctcccggac
      241 ttccttgaag tcatctacac cgatgggcat ttgagtattc atggtttcac ctgctttaga
      301 caagaattac ggactacgga ttgactctat tgtagcaaaa ccggccggat ttgtatattt
      361 agaaaaccaa gagcttgtcg cacgtgcgat aagccttttt cctctacggt tgtttgccga
      421 tataggcaag gatgccgccg tcgacatata agatatggcc attgacgaag tccgacgcat
      481 gggaagctaa gaatacagct ggcccttcga ggtcttctgt cgtgccccag cggcctgctg
      541 gcgtcttgga gacgatgaac tggtcaaaag gatggcggga gccattaggc tgtttttcac
      601 gtagcggagc tgtctgaggc gttgcgatat agccagggcc aatgccattg cactgaatat
      661 tggcgccacc aaattcagcg cagatattgc gggttaacat tttcagaccg cctttggctg
      721 cggcataagc cgatacggtt tcacggccta attcgctcat catagagcag atattgatga
      781 ttttgccgtg gcctttttta atcatgccgg ggatgaccgc tttggatacg atgaacggcc
      841 cgactaagtc gatatcgacg acctggcgaa actcttccgt cgtcatgtcg cacatgggga
      901 tgcgtttgat gatgccggca ttattgacga gaatgtcgat ggttcctaac gtcttttcta
      961 tttcgctgac taattgctgc acatgaattt catcagtgac atcgcaaata tagccgtgtg
     1021 cgtcgatgcc ctctctttta taatccttca tagcctggtc caagtgttcc tggctgcggc
     1081 agttgaaggc gatgacggcg ccggcgcggg ctaaggcttt ggcgatagca aacccgatgc
     1141 cgtaagaacc gcccgtaaca agggcgacat tgcctttcaa agaaaacata ctatttagat
     1201 aatccatgtc gagcctcctg aaaataagaa tggtactata ttttttgtaa aatattcgat
     1261 gagatatggt gtatatcggg aacacctgtt atcatcatag catcttttaa ttcttctttt
     1321 aaggtattga gaagcaaggt aaccccttca gcacctccgc cgatattggc aatcgcagca
     1381 ggacggccaa tcaggactgc atctgcgccg agtgccaatg cttttaagac atcttcgccg
     1441 tgccggatgc cgccgtctac gaaaatggtt aacttatttt ttacgacagc cgaaatttcg
     1501 ggaagtaccg acagagagct gaccatacca tcgagtatac gaccgccgtg attgctgact
     1561 acgattcctt tggcgcctgc ttgcaggcaa agcagggctt cgtcagggga catgatgcct
     1621 tttacgataa atgggagatg aatggaattg ataatgtgtt ttaggttttc taggcgcttc
     1681 acttctaagg gttggccaaa aaaacgcata ttggctgatg ttacggcatc aatgtcgttg
     1741 gcgactgcga atgcgcctga ttgttcggct aatcgagctt tttctatgat ttttaggtct
     1801 tttcgcggct taattgtcgg gattacctgt ccaggatatc tctttgaagc ggcaattccg
     1861 gctgcataca tataagcatg gccgctgtca ccggtaaatg ccagtgttcc tgcttttgtg
     1921 acaccttcaa tcatcgaaaa gaggtattcg tattcttttt cttctggatt tccttctact
     1981 tttccattat ggacatagcc gctgactggg gcgacaagaa tcggcatgga taaagttttt
     2041 ccaaaaatgg aacatgtagt gtctggattt ttactagcag ataggagatg agatacgaga
     2101 ccgtattttt gaaaggccaa tcgg
//
LOCUS       DAWABZ010000210         2121 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_10249_length_2121_cov_3.928848, whole genome shotgun sequence.
ACCESSION   DAWABZ010000210 DAWABZ010000000
VERSION     DAWABZ010000210.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10249_length_2121_cov_3.928848"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    314..323
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          459 a          619 c          537 g          496 t
ORIGIN      
        1 gtcaaaggca ttatatttaa cgaacccgat tttacggaaa cagtgattct ggacagccat
       61 gatatccttc tgcgtctggt ggatcatgtc gagtgtcccc tgcaggttat gcagcttatc
      121 gacatcgtca cgcagcttcg cttcgacgct ggctcccgtt tcgtcttgca taaaaaaggt
      181 gtaattcgtt tctaattttc tgattttctt gtgtaaaatg acgatataga cgagcaagag
      241 caataaaatc aacccgccaa taccggcaag cgccatcgtg gcagcctgca tatcgacatt
      301 cagcatttct ttcnnnnnnn nnncctccca tgaacatgaa tatgcaaaaa tattatatat
      361 gaaacgaaga aagaggtgac cgcggcgtgt cttctttttc ttcagtcagt atcgacagca
      421 gccgttcaaa ttctgcttct gacgaaaagg aaatctcgat tttccccttt ttccggccct
      481 tccccagctg gatggccaca tcggtcccca aggccatctt cagcttatcc tgcagccagg
      541 ccacatgggc gtccggttcc gggcggggct tggcctgggt catcagatgg cggaccaggg
      601 cttctgcctt gcgggccgac agctcatgct tcataatgta cagagcagct tcccgctgca
      661 gtttcgcaga cggcagctgc aacagaggcc gggcctgtcc catggatagg cggcctgcag
      721 cgatccattc cttgatgtcg gctggaagct gcagcagacg gaccatgttg gccacatggg
      781 accggctttt cccgactttc tgcgccgtct gttcctgtgt caggccaaag tccttcatca
      841 gccgttcata ggccatgcct tcttcaatag catccaggtc ctggcgctgc aggttctcga
      901 tgagagcaat ttctgccgct tcatcgggcg tatagcttcg tatgatagcc ggcactgttt
      961 tcaggccaca gcgtttggcc gcccgcagcc gccgctcacc ggcaatgagc tcatatcggc
     1021 cgggaccggc cttctggaca acgacaggct ggacaagccc atgctgacgg atggaatccg
     1081 ctaaagcggc cagcccctgt tcatcaaagg tctgccgcgg ctgccgggga ttggggacga
     1141 tacattctac ggcgatttcc gccggtgcgc cccgttcagc aggagactcg tcgactgcca
     1201 ccatgtccac ctcatttctt ccaaaattat ttttgaagtc tatattatag tatatcgtct
     1261 atccaccatt taatcaaata tttattgccc tgatttcatt gatttttcta acaatttcta
     1321 tggtataata agaagcaaat gtacagattc agaaaggatg taaaaaatgc caacatttac
     1381 cgaccccttg cgggaaatcg acgctgccag cgtcaaagaa tacgccttgc agcgcggagc
     1441 tgctgaactt tccgatgatt tcgtccagga agcgtgccag cgcgtcatgg aactggccaa
     1501 accccaggct acgtgccagc agtgcttcta tgacagcacg tcccatatgg tcctttgcga
     1561 cagccccttt accatcaaca gcccgactgt ccaccagcgc atcgaagatt cggccatcgt
     1621 cctgatgtat gccgttaccc tcggcgaagc cctggaaaaa gaagtcgatg ccctgttcat
     1681 gaataaagac atcacccgcg gcctggctct cgattcggcc ctggccgtcg ccacagcagg
     1741 ctatacgaaa cagctcatcg acatgctcga cgaagccggc gccgataaag gctataaagc
     1801 cgcctggatc atgaatcccg gcactggcga ctggccgtcc ggccagatgg ccaacatcgc
     1861 ccaggccgtc cacgccgaac aaatcggcgt ctccctcctg cccagcggca tgctcatgcc
     1921 acgcaaaacc gtaaccggcg tcatcggcct gaacttcatc ggcggtggct gctccggttc
     1981 ctgcagctcc tgcgccatgg ccggccattg cggggaatag ggctaggggt taggggctag
     2041 gggctcgtgg ttcgtggctc gtggttcgtg gctcgtaggg gccgtcccaa agggcgtccc
     2101 gctacacgct gagggtttcc c
//
LOCUS       DAWABZ010000211         2089 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_10395_length_2089_cov_2.557522, whole genome shotgun sequence.
ACCESSION   DAWABZ010000211 DAWABZ010000000
VERSION     DAWABZ010000211.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10395_length_2089_cov_2.557522"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    519..528
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1774..1783
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          566 a          488 c          514 g          501 t
ORIGIN      
        1 ccatggacga gtgcgcggac gagagggcgg tcagaggccg aaacgatgta gcaggtgaaa
       61 ttattagctt ctaaatagga aatcacttct accataggca ggtaaaagga ttcgccataa
      121 gtcaggttcg tcaggccgat aacgggcttt ttcataaatc cctgggcgta agcgtccagg
      181 gactgcggcg tcatcccggc cagtccggca tataagttgt ggtacaagtt cttttcgatt
      241 tccgggggaa cgggtttccg ctgagaaatg gcatcccaat aggcttgagc aaattttttg
      301 tcttcatcgg acactttaca gtttgggtca tgcaggacac ggtccgcata aaagacgcca
      361 tctgtatagg acggggtccg ttctcccaac agggttccat ccatatcgaa gacggccagg
      421 cgatgttctg ccgggatgaa atcaggattt ttgggattcg ttactttttg cacataagct
      481 accagctttt tcttagccgg agaattctcg ttccaatann nnnnnnnntt cggactgatt
      541 gacgtgaatc gcggctaatt cggcccggct ggcaccttca acaatccccg tcaggccgaa
      601 gctggtcaac atagctgtga cagctaacat ggatgcgagt ttttttcctt tcaacataat
      661 cacctcaaat atagtaaaat ttgtctctat tataacacat ggatttcgtt gaattaaata
      721 ggacagatgg aagaaaaact ggcatttcat cgaaatgggc ggccagacag gtcttacgta
      781 cacgatgaaa tatattccga agaaaggcag gggaaagcag gaattaatgc ctgacgtgta
      841 gaattattcc ataaagcttg tttgccagat gatgggtgag gcgtgacgta cactctgcga
      901 ggtgatgata tgtctttgga aaatcaggaa ttggcctgcg tgcaggcttt tttagaagac
      961 cgggcttcag aacaattcat gacgcggctg ttggccgatg ccggagaaga ctatgcatgg
     1021 ttccggcgcc tggctcagca tatgttccgc agccaccttt cgccagacga acaacgggag
     1081 ctcttggaag cggcaaaggc ggatacgcag ggcgaccccg aaaaaatcca ggcggcccat
     1141 gatgcccgct tgttgctgct gaaatcgggg atgtgtcttt ccatccatga atcgtggtat
     1201 ctccataaag tgaagcaaat gcccttagaa aaagcctttc gccaaaccat gacgcgcatt
     1261 gtcgtaggct tcggccagct catgtcagaa ccggggattc ccaacgattt tgaaatgcag
     1321 ctatggtatc ggattcaagg atagcattgc atataagaga agggagtagg atactattga
     1381 atcagaaaga gtgggaccgc atgattggcc aggagctgta cgacccgttc aatgttcacg
     1441 atgacagctt ccagcgggtc catgcggcgc agcggaaatt caatgcttct gacgatttga
     1501 acgaccgtca ggcttttgaa gaactgaaac gctgttttgc ccaggcgcca gatgacatgg
     1561 tgctgttggc gccggtctac ttcgaccatg gtgaccgcat ccgctttggc cgtcatttct
     1621 ttgccaatac ggggctgacc atactagacg aaaactacgt cacctttggc gacaacgtct
     1681 ttttggcacc tcacgtcagt atctatacgg cgggtcatcc cattgatgcg gaagtccgca
     1741 ataccaacct ggaatacgcc aaaccggtca ctannnnnnn nnngtcgtaa cccatgatat
     1801 tcccagccat gtcgtcgcag ccgggaatcc ctgccgggtc atccgtccca ttacacaagc
     1861 cgatgcagac ttatggcatt gtaagttatc ggaatataaa aaatcaatat tatcccacat
     1921 aatcccacaa aaacaaccaa ggaaaaacca tatacccgaa aaacaaatcg aaaaaccata
     1981 tacccgaaaa acaaatcatt gcaaaaaaaa ttaacgattg gttaaacgct tgtgttgatg
     2041 agagcggtgc tttctttttg aataccatag aagctggccg ccctaaatc
//
LOCUS       DAWABZ010000212         2069 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_10485_length_2069_cov_3.125124, whole genome shotgun sequence.
ACCESSION   DAWABZ010000212 DAWABZ010000000
VERSION     DAWABZ010000212.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10485_length_2069_cov_3.125124"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          523 a          455 c          622 g          469 t
ORIGIN      
        1 gatttctatt tcgtaaactg cagggggact gtcgatacga cgacgtctaa gtaaggcagg
       61 atacggagtc ttagcagcca gccgctctgg ttgtgaagca ggcggtgcca taattttttc
      121 gtttcaaatt ccggaatgag gacggtaatc atatctttcg gagccgtctc tttccgcagc
      181 tcttggataa aggtgatgag cgggtccgtc aactgccgat aaggcgattg aagcatgatg
      241 agctcgatgt cgggttccag ttggcgccac aaggttttga tttttttacc ccgttcatcg
      301 tcgctgtaga cgtggacggc atagatttta tctttcgaac cgctgatgat cttggcgtag
      361 cgaatggcct tggctacggc ttgagtcggg gacgcgatgg ggatgatgat gatattgcgg
      421 cctaagtcgg tggtcatgac tttcgtatac tcttccggtg tcagcaacag ctgttctcga
      481 acgtcatcat agtgatgatg gactttaagg aacatataga ccaaggtcgg gatgagcagg
      541 acgatgatcc aggctccgta gatgaatttg gtgatgagga cgatgacgac gacgcaggcc
      601 gtgaccaggg caccgaagcc gttgatgacg gaaaagaggc gccagtttcg ttcccggcat
      661 tgtctccagt agacgaccat ggccgattgg gcaatggtga aggagaggaa tacgccgatg
      721 gcatagaggg aaatgaggtg ctcaatatta ccctggaaga ggacaatcaa gatgccggcc
      781 atgagcgtca ggacttggat gccgttagaa tagctcaggc gttcaccgcg gttggcaaag
      841 tagcggggga cgtagccgtc gacggccatg aaagaaagaa gcggcggcag gccgttgtag
      901 gccgtattgg cggcgatgta gaggatgacc atcatcaaaa tctggatgac gtagtaaatc
      961 aaggtccggc tgaagatatc ttcgacaagg agggacaata gggtgtagcc ttgtgtcggc
     1021 aagaggtgaa gataaatgat gaggtaaccg aggccgccga gcatgattgc caggatacag
     1081 gccatgacag ccgtcgtttc gatggcgttt ttttgctgag gcgccttgaa catggtgacg
     1141 ccgttggcaa tggcttcgat gccggtcatg gaactacagc cgttagcaaa ggcccgcagg
     1201 acgatgagca tcaaggcggt ctgattcaat ggggctaccg tttcgacagc gacggcatga
     1261 gccgtgaggg tgccgttcca gatttgatag aagccgccga ttaaggtgat ccccatggca
     1321 gtcaagaaga agtacgtcgg ccagacgaag acggttgagg cctccctggt accgcgcagg
     1381 ttgacgatca tcatcaggat gagaacgccg aggtcaaggt agacgtgata agggtggagg
     1441 accggaaagg cactgaccag cgccgccgtc cctgaagaaa tggaaacggc aacggtcagg
     1501 atgtaatcgg ccagaagcgc cgcccctgcc gtaagggccg ggtagacacc gaggtatttc
     1561 atggcgatgc cataggaacc gccgccgccc ggattgaccc ggacgacctg ggtataggac
     1621 agggcaacga tgaataataa ggcaatgata gctaagacga taggtccgaa ataacggtat
     1681 gtcgctaagg tcggtaccga agccaggacg atggcaatct gttccggccc gtacccgacg
     1741 gatgacaggg catccgatga gaaaataggc agggccttcc attttggcat ctgttcgccc
     1801 tcgagtttgg tattctccaa aggcttgccg agaaggtggc gactaatcgt tttgaatagc
     1861 atgatagcct ccttttttta ttgtaagaaa ctgcattttg ctaaatacag ctcagtataa
     1921 tcccttttca tactcctgtc aataaaggtt tttcatgtgt tattcctgtt gaccataaga
     1981 tttcagaaac aatagcaaaa agacatccct cgctgctgca agtcaaagga tgcctttttg
     2041 tctaaggagg aaatcgtgtt atttagagg
//
LOCUS       DAWABZ010000213         2054 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_10564_length_2054_cov_2.604802, whole genome shotgun sequence.
ACCESSION   DAWABZ010000213 DAWABZ010000000
VERSION     DAWABZ010000213.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10564_length_2054_cov_2.604802"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          579 a          535 c          519 g          421 t
ORIGIN      
        1 accacagccc gttcgggtat aataacagaa ttttacgatg gatacgaaaa tggcgatgtc
       61 gtcagtttga gtgtctgccg ccgcgttgcg gcatcggagt ccgggcggcc tgtgaactgt
      121 gctctgtgtc ctcattttcc taaatctact ggaaacagtt gcaaaatagg cgctaatcac
      181 ggtataatgt gatattgaag gcatttatac atatatgaaa gggagataca tcgatggata
      241 acgaaacgac caaaaatttt attgaaacta ttatcgatga agacaacgaa aagaatacat
      301 atggcaagcg ggtccatacc cgtttcccgc cggaaccgaa tggctatctc cacatcggcc
      361 acgccaagtc gatctgcctg aatttcggta ttgccaagaa atatggcgga ataacgaatc
      421 tccgctttga tgacacgaat ccgtcgaaag aaaatacgga atatgtagaa tcgattaaac
      481 gcgatgtcaa atggctcggt tttgactggg acgaccgcat gttctatgcg tcggactatt
      541 tcgatagact ctatgaatac gccaagaaat tgatccgcct gggcaaggct tatgtcgacg
      601 atttgtcgag tgatgaaatc cgcgaatacc gcggcacgtt caatacgccg gggaaggaaa
      661 gcccgtaccg taaccgcagc gtcgaagaaa acctccagct ctttgaagat atgaaagccg
      721 gcaagttcgc cgatagcgaa aaggtactgc gcgccaagat cgatatggcc agcccgaacc
      781 tcaacatgcg cgatccggtc ctttaccgta tcgtccatgc ttcgcaccat cggacaggcg
      841 acaaatggtg catctatccg atgtacgact tcgcccatcc cgtatcggac gccatcgaac
      901 gcattactca ttctatctgc accttggaat ttgaagctca ccgtccgctg tacaactggg
      961 tcctcgaaga ctgggacgat ccggaaggcc agaaaccgcg gcagatcgaa ttcgcccgcc
     1021 tgaatgtcac taacatgatc acgagcaagc gtaaactgcg cctcctcgtc gaaaagggca
     1081 tcgtcagcgg ctgggatgat ccccgtatgc cgacggtttc cggcatccgc cgccgcggct
     1141 atacgccgga atcaatccgc gacttctgcg aacgcatcgg cgtcgccaag gcagacagca
     1201 tggtcgatat ttccctcctg gaatactgca tccgcgaaga cctgaagatg aaagcaccgc
     1261 gcttgatgac ggtcatcgac ccgatcaagg tcgtcctcac caattatcca gacggcaaga
     1321 cggaaacgat gacggccgac aacaatcagg aaaatgaagc catgggcacg cgcgaaatga
     1381 cgttcagcaa gaacctttat atcgaacgca acgacttcat ggaagaaccg gtcaagaagt
     1441 tcttccgcct ggctccgggc aaggaagtcc gcctcaagta tgcatacatc atcaaatgcg
     1501 aagaagtcat caaagacgaa gatggcaaca tcgtcgaact gcactgcacg tatgacccgg
     1561 aaagcaagag cggcagcggt gccaacgcca accgcaaggt caaagggacg ttgcactggg
     1621 tcaatgccga agatgctgtc gacgtcgaaa cccgcgtcta tgactatctc ttgaaggacg
     1681 acggcagcga ggaagaaggg gaaagcaaga atttcctcga ccagatcaat ccccacagcc
     1741 tcgacgtata ccacagcaag gccgaaaagg cattggcaac gtatcatgtc ggtgataagt
     1801 tccagttcct gcgccagggg tatttcgtca ttgatcccga tacgacggca gaccacctgg
     1861 tcgtcaaccg catcgtcggc ctccgcgaca cctgggctaa gatgcagaaa aagaaaaaac
     1921 aataagcgat tattaaaaaa ggggctgcct ttctcagaat ggattagaat ttcatgagaa
     1981 agggcggccc tttcgtatct tgcgtgtata atggaggtag gccatgaaaa cccctggctc
     2041 acacagtctg ttgt
//
LOCUS       DAWABZ010000214         2049 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_10587_length_2049_cov_2.880140, whole genome shotgun sequence.
ACCESSION   DAWABZ010000214 DAWABZ010000000
VERSION     DAWABZ010000214.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10587_length_2049_cov_2.880140"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          486 a          568 c          510 g          485 t
ORIGIN      
        1 aatagtgaca ccggcacctg ccaacaacag gggcaatgag ttccaaatca gactaaaatc
       61 aaacgacatg ataacactcc tctaaatgac actctataac atatgataat atatacgcta
      121 ctctattata cgtgcatatt tataagatgt caacgcattt tccctgtctc ccttcatagg
      181 ccctttggga cctgtcaggt gctgaccgca ggaaagagca ggccagttgc caaaagggag
      241 cgccgagctg gaggtggcct taagggccgg agacgttgca ctaaaaaaag ttgtcgcatg
      301 acggcgagaa ccttcatgcg acaacctctt tccaaacctc tcagtcagct atgctgacag
      361 ctcccctatc aagggagcca caaactacga gccacgaacg cctatctccc caggagatgg
      421 acgaggtgga acaggttttc gtcgatggga tagcgcaggg cttcggcttc cgaatacggt
      481 gtcgctacga cgcggttgtc gatgaggccg acgacgcagt tatacgtgcc agccagcagg
      541 ctgtcgacgg ctttctgagc gaaacggccg gccatgatgg catcgtaagc cgacggggcc
      601 ccgccgcgct gaacgtagcc gaggacggtc agattcggcg acaaatacgt atgttccttg
      661 atataatcga cgacgtccgc ccctttggca gcgccttctg cgacgatgac gatgctgctc
      721 agtttcttca tgcggtgcga ttcttccagg tgacggcaga gggcagccag atcgacggga
      781 tgttccggga ccaggaccgc ttcggcgccg gaagccatgc cggcgttgag ggcgatgaaa
      841 ccggcttcac ggcccatgac ttcgatgacg gcgacgcggt cgtgggagaa ggccgtatcg
      901 cggattttat tgaccgattc caggacggtg ttgaccgtcg tgtcgaagcc cagggtatac
      961 tgcgtcccca tcatgtcgtt atcaatggtg cccggaatcg taatcgtcgg gaatcccaac
     1021 cggctcagtg cctgggcacc acgcatggaa ccgtcgccac caatgacgac gagagcatct
     1081 atttccagtt cacgcaaata agcaacggct tttttctgcg tttctttttt tttgaattcc
     1141 aggcagcggg ctgtccgaag catggtacca ccgcgatgga tgatagcgcc gacatcacga
     1201 cggcccaagg gacgcgtttc atgactcagc agcccttcat aaccgcgaat gatgccttcc
     1261 accgtgaggc ctttctgaat ggcataccgc gtgacgccgc ggatagccgc attcataccg
     1321 ggggcatccc caccgctggt caatactccg attttgttga tcatttttca tcaatctcct
     1381 ttttttccct ggcaatgaat tgcacatgcc tttcgttaat tttatggaca atggattcga
     1441 cgatctgtga tgctcccccg cgcgtcgtat caatgacgca gtcgcagtac ggatagacct
     1501 cttcccactg tttcatcatc gtgtggacga aatcatgaag attttcatag tcgacagtcg
     1561 gccgcttgcc tatatggcgg ctgacccgtt cttccacgcg gaacggttca gacaataagg
     1621 cgacgataaa cccatggcga tgcaggattc gataagtacc gacattcata ggaaaattgc
     1681 cgccaaagga aatgacagct tcatgatagc ggctgacttg ctgcaggaca ttgatttctc
     1741 gttcgcgaaa cactttttcc ccggagctcg caaagaactc ggcaatggtc atgcccacag
     1801 cccgttcaat acgccggtcc gtatcgacaa attgccagcc gaggcgctga gccagcttgc
     1861 ggccaatatg actcttgccg ctccccatca tgccgacgag aacgatattc cgcttcatca
     1921 ttatcggcgc acctcctcaa attagtatac tctgtatatg taccccctta ttctatcata
     1981 attttttctc cttgacacaa aaaactctgt actttttctt catatatcta taaatggagc
     2041 gaatttttc
//
LOCUS       DAWABZ010000215         2049 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_10586_length_2049_cov_4.441825, whole genome shotgun sequence.
ACCESSION   DAWABZ010000215 DAWABZ010000000
VERSION     DAWABZ010000215.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10586_length_2049_cov_4.441825"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1827..1836
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          546 a          557 c          503 g          433 t
ORIGIN      
        1 cataaagtgt aaaggatgtc cttgcacaaa atgtaaacga tgttattctt gacaaatgtc
       61 ttgaccttac atcacaagct gcaatccggc aatttcggga cataccttta ttccttaaaa
      121 aaagtgaaaa agagtgcttg aataaccata tacatagcaa aggcacacgt tttttgccga
      181 ctggatggcc ggtctgtttc gtgtaccttt tttacgttta gctaaaaaaa gagaatagaa
      241 cgatgaaata gaataaaaaa agagagctgt cgcacaaagc cgaaatggct taatgcgaca
      301 gccccttttt tttacgaacc acgaatcacg agcgatgaac caccaggcgt cacggatgta
      361 ggggcttgca cgtggcaagc ccgttccaga ctatgagtga tttcccagct cggagcgaat
      421 gccacccaag gtccggcggg tcgcccacgt ggcatatgcc aaaacggcat atacctgttt
      481 acctaaacgc cttgccaggg cgccttcagc gtcgtataat aagaatataa gcaaacgtaa
      541 aacgtcgtac taaagaaaat acatacagat gaaagggtgg tttttatgtc ccaatggaca
      601 gatatgtaca gacagaaatt aatgacgccg gaagaagcgg taaaagtggt ccagtccgga
      661 gattgggtcg attacggcat gggcacgacc cagccgatcc tcctcgacca ggccctggca
      721 gcgcgccgtg atgaactgca agatgtcaaa gtccgcatgt gcctgagcgt ggctccgcgg
      781 cagattattg aacaggaccc ggagcgcaag gcctttacgg ccatgaactg gtatatgagc
      841 ggctatgacc ggaagaagtg cgcccttggc cagatgaatt tcatacccat gtgttaccgc
      901 aacaagccgt ccatgtaccg cgacctcctc gacgtcgacg tagccctgat tactgtcggt
      961 cccatggata aacacggctt ctttaatttc ggcccggccg tttcggcaac ggaagccatt
     1021 accaagaaag cgaagaaagt catcgtcgaa gtcaatgaag ccatgccccg cgtcctcggc
     1081 ggccgcggcg aatgtatcca tatcagcgat atcgacggca tcgtcgaata cggcaatcat
     1141 cctttggcga ccattccctt tacgacaggc gacgatatcg atgcccagat tgccaagacc
     1201 atcgtccagc agattcccga cggagcgacg ctgcagctcg gcatcggcag tttgcccaac
     1261 acgatcggcg ccttattggc cgaatcggac ctgaaagacc tgggcgtcca tacggaaatg
     1321 ctcgtcgacg ccttccgact catgtatgaa cacggccagc tcacgaaccg ccgcaagact
     1381 ttctgccgcg ataagataag ctgggccttc gccctgggga cgcaggacct ctatgactgg
     1441 atggacgaca accccttcct ggcagcttat ccggtagacg tcatcaatga tcctttcatc
     1501 atttctcaga tggataattt catttccatc aataactgca tcgacatcga cctcttcggc
     1561 caggtatcgt ccgaatcagc cggtacccat cagataagcg gcagcggcgg ccagttggac
     1621 ttcaccgacg gcgcttaccg ctcccgtggc ggcaagagca tcatcgcttt gcggtcgacg
     1681 ttccacaaca agaagaccgg ccgcgatgaa tcgcgcatca tcccgacgct ggccccgggc
     1741 acgacggtca cggacccgcg gtcccaggtc aactgggtcg ccacggaata cggcatagtc
     1801 aacctcatgg gcgcttcgac atgggannnn nnnnnncctc atcagcatcg cccatccggc
     1861 tttccgcgaa gacctgatca aagaagctga aaaaatgaag atctggagat acagcaataa
     1921 gaaataaagg gatacgctat tattctggta agtgttccca cggtatgctc tcgttcgtcg
     1981 taggtcgttc atcgttcgtc gaaaattaaa aagtaattac gatgaactgc cagcccctaa
     2041 cccctaacc
//
LOCUS       DAWABZ010000216         2048 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_10593_length_2048_cov_2.297541, whole genome shotgun sequence.
ACCESSION   DAWABZ010000216 DAWABZ010000000
VERSION     DAWABZ010000216.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10593_length_2048_cov_2.297541"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    741..750
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          545 a          480 c          476 g          537 t
ORIGIN      
        1 gtaattcgct gacgtcagca taggtctggt atacatcacc aggctgcatg ggcagatatt
       61 ctttttctgc cgttttgccc agggcctttt ccaggacttc aataaaatgc atgagctgtt
      121 ccggtttatg gttgccaatg ttatagattt tatacgaatc gcctagttca tttttcttcg
      181 gcggattgca cagcatgtgt tcaatgccgg tgacaatatc atcgacatag gtgaagtccc
      241 gatacatgtc gccgtgatta taaatccgga ttgcctctcc tctgcgaatc ttattggcaa
      301 atttgaaata agccatgtcg gggcggccga aaggcccata gacggtaaag aaacgcaacc
      361 ctgtcgccgg gatgccatag agatggctat atgtgtaagc catgagttcg tcggacttct
      421 tcgtcgctgc atagagactg atgggatggt cgacattatc ggtcgtcgaa aaaggcgtct
      481 tctgctgatt gccatagacc gaagaactgg aagcaaagag caaatgagat accttatgat
      541 gacggcaagc ttcaaggata ttaaagaaac cgataatatt ggaatcaata taggaacggg
      601 gatgatcgat gctgtaacgc actccggcct gggctgccag gtttacgaca atctccggct
      661 ggaacgacgt gaaaacatca ttcacgactt tttcatcagc caagtcccct ttgacgaaag
      721 taaatgtcgg gaactggcgt nnnnnnnnnn gcgtagaata gccaaccggc tttctttcag
      781 ggaaacatca taataatcgt taaggttatc caggccaagg atggtggccc ccatggtcag
      841 caggcgcttg gccaaatgga atccaatgaa accggcggca ccggtaatca agactttgtt
      901 tgcactatca aaaggtttaa aatctgccat tatcatcgac tccctttcag tcgcggtcaa
      961 agatatcccg cgtatagact ttatcctgaa cgtcgctaag ttcttctgcc cagcggttgg
     1021 aaataataat atcgctcatc ttcttgaact cgttcaagtc gcggatgacg cgggaatgga
     1081 aaaagccatc cgctttcagt gtcggttcat agacgactac ttcgatgcct ttggccttga
     1141 tgcgcttcat gacaccctgg atggccgagg cccggaaatt atctgaatgg ctcttcatcg
     1201 tcagccgata gacgccgacg atgcggggat tcttggcgat gatcatatcg gcaatgtggt
     1261 ccttccgcgt ccggttggca gcgacaatgg ccgaaatgag gttctgcggg acatcctgat
     1321 aattggccaa aagctgcttc gtatccttcg gcaggcagta tccgccgtag ccaaaggaag
     1381 gattgttata aaaatcacca atacggggat cgaggcagac gccttcgatg atttccttcg
     1441 tactcaggct acgcatttca gcatacgaat cgagttcatt gaaataggct acgcgcaaag
     1501 ccagatatgt attggcaaag agtttgatag cttcggcttc cgtggaacca gtaaacaaca
     1561 tggggatatc cttcttcacc gcgccttcgg ccagcagatt ggcaaagact ttagcccgtt
     1621 ccgaccgttc accgacgacg atacgcgacg gatgaagatt atcatacagg gccttgcctt
     1681 cccgcaaaaa ttccggcgaa aacataatat tgtcggtatg gtagcgttcc ttgatatgct
     1741 gagtgtagcc gacaggaact gtcgatttaa tgaccatggc cgcttccgga ttgatataaa
     1801 taagcatcat gatttcagac atgtcataca tcatcacgct gcaaaaaagc cgagatgtcg
     1861 ctatctataa tcggcgattt tccttcattc aatatagcca ctttttctgg aataatatct
     1921 aaagcaatga catcattatg ctgtgccaac aacatactat ttgaaagacc gacatacccg
     1981 gtcccggcaa tcgctatatt catataaatt cctactttcc ttttagcaca ttatatttat
     2041 tataccat
//
LOCUS       DAWABZ010000217         1979 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_10938_length_1979_cov_2.760915, whole genome shotgun sequence.
ACCESSION   DAWABZ010000217 DAWABZ010000000
VERSION     DAWABZ010000217.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10938_length_1979_cov_2.760915"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    515..524
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          395 a          639 c          518 g          417 t
ORIGIN      
        1 gcagcatgct cggcgcgtcg cagttcacca ttttccggcg catcatcttc ccggaactgc
       61 ggccggctct gttgacgggc ttcggcctgg cttttgcccg gggcctcggc gaatacggca
      121 gcgtcatcta catttctggc aacagtgcca agcatcatac gcaggtcgtt tcttacgtca
      181 tcatgcagaa gctcaattac gtcgattacg ccagcgccac ggccatcgcc ctggccatgc
      241 tggtcatcgc ctttgtcaca ctgttttcta tcaatatgat ccaggtccat caggcccggc
      301 gcgtccagta gaggagggga gccattatga aacacagtaa gacggaaatc gccttgctca
      361 cgatcagcgt cgtcttcatc gtggtcatgc tcatcgttcc cctcgtgtcg gtcctcacca
      421 actcgctggc cgaaggctgg gacttttacg tacaagccct gacgacgcca tacgtcctgt
      481 cggccctgcg cctgaccctg atcgccacgg tcgcnnnnnn nnnnttccaa attcgacttc
      541 cggggcaagc atttcctgac gaccctcatc gatataccct tttccatctc gccggtcatc
      601 gccggcctgg ccttcatcat gaccttcggc cgcatgggct ggatcggtca gtggatcgaa
      661 gtcctcaacg actactgggg gacggatatc caaatcgtct ttgccgttcc cggcgtcatc
      721 ttggcgacga ttttcgtcac ctttcccttc gtcttccggg aaatcatccc cgtcctcagc
      781 accaacgggc gcgatgaaga ggaagcggcg gccctcatgg gagccagcgg ctggactatt
      841 ttcttccgca tcaccctgcc gcagatcaaa tgggccctgc tctacggcat cgtcctctgc
      901 acggcccggg cgctgggcga attcggtgcc gtcagtgcct tgtcgaaact gcgcggcgag
      961 acttttacgc tgcccctgga aatcgacgcc ctctatcaat cggggtcggc cgatgccatt
     1021 acggcggcct ttgccgtatc gtcactgctc gtcttattag ccatcatcgt cctgctgctc
     1081 cggactttcc tcgaagccaa ggccgataaa acgtcataaa ggaggtgaag ctcatgtacg
     1141 tcgaaatgaa acacatcgaa aagaattatg ggtccttcca cgcgtcgcgc gacgtttcct
     1201 ttggcatcga caagggcaag ctcgtcgccc tcctcggtcc cagcggcagc ggcaagacga
     1261 cgatcctgcg catgctggcc ggcctggaga atcccaacag cggcgacatc ttcatcgacg
     1321 gccgccgggt caacgacatt cccgctgcca agcggggcat cggcttcgtc ttccagaact
     1381 acgccttgtt ccgctatatg accgtctacg acaacgtcgc cttcggcctg gaaatccaga
     1441 agcggccgaa agaggagata cgccagcgcg tattggagat gatcgacctc gtcggcctgt
     1501 cgggcatgga aaagcgctat cccaaccagc tctccggcgg ccagcgccag cgcgtggcct
     1561 ttgcccgggc cctggcgccg aatccccagg tcctgctcct ggatgaacct tttgccgcca
     1621 tcgacgccaa ggtccgtcag gaactgcgca actggctcaa ggatacgatt caccgcgtcg
     1681 gcattaccag catcttcgtc acccacgacc aggacgaagc cgtcgaagtg gccgatgaaa
     1741 tcatcatcac caaccacggc catatcgaac aggtcgggac gccgctggat atttaccgcc
     1801 agcccaggac gccctttgtg gctcgattta tcggaactcc agctgtcatc aacaactgca
     1861 acatcctcac gggattcctg caagtcggaa cgggcaaaca agccatcgtc cggccggagt
     1921 tcctcaagat ttacaaacac ggcacgatga agcagtatat cagtgccgct gaagaaggg
//
LOCUS       DAWABZ010000218         1974 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_10965_length_1974_cov_4.111516, whole genome shotgun sequence.
ACCESSION   DAWABZ010000218 DAWABZ010000000
VERSION     DAWABZ010000218.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10965_length_1974_cov_4.111516"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          295 a          524 c          608 g          547 t
ORIGIN      
        1 ttccttgaca taggaaccgc cgacgatgac tttcggcgtc gtaatgactt tacggacttc
       61 agcacggccg acgatctttt cttcgtattt cttttccagc atccctttga tagccgcttc
      121 gacgtcgttg atggcgtcgt aaatgacgcg gtacatgcgc atgtcgactt tttcatgttc
      181 tgccatcttg cgggctgccg catcggggcg gacgttgaag ccgatgatca gggcgttcga
      241 tgccgaagcc agcatgatat ccgattcgtt aatggcgccg acgccggcat ggacgatgga
      301 gatgcggact tcgtcgctct tgatgcccat gagggactgg ctcacggctt cgacggaacc
      361 ctggacgtcg cctttgatga tgaggggcag ttccttcagt tcgccctgct ggatctggtt
      421 gaacaggtca tccagggtga ctttggcatt gacgtggagt gcggacgaac gtttcttttc
      481 caggcgtttt tcggcaacgg aacgggccgt cttttcgtcg gtgacgttga ggatgtcgcc
      541 ggcttccggt acgtcgttga ggccgaggat ttcgactggg atggacggtt ccgctttctt
      601 gacgcgttcg ccccgttcgt tgttcatggc gcggaccttg ccgaagcagg tgccgacgat
      661 gatcgtatcg ccgacgcgga gggtcccttt ctggatgagg acggaagcga ccgggccgcg
      721 gcctttatcc agcttggctt cgatgacgac accctgagcc ggacggttcg ggttggcctt
      781 gagatcctgt acttctgcca ggacgaggat attttccaag agatcgtcga ggccgatttt
      841 cttatgtgcc gaaacgggga ccatgatggt gtcgccgccc cagtcttcac tgaccaggcc
      901 gtattccgtc agctgctgca tgacgtgttc cggattggcg ctttccttat cgatcttgtt
      961 gatagcgacg atgatgggga cgccagccga tttggcatgg ttgatggatt cgacagtctg
     1021 cggcatgaca ccgtcatcag ctgcgacgac gaggacggcg atatccgtga cctgggcacc
     1081 gcgggcacgc atagccgtga aagcttcgtg gcccggcgta tccatgaagg tgattttctt
     1141 gccctggtag cggacctgat aagccccgat gtgctgagta atgccgccgg cttcgtggct
     1201 ggtgacattg gtctgacgga tggcgtcgag gaggctcgtc ttgccgtggt cgacgtggcc
     1261 catgatggtg acgacaggcg gacgggtctt gagggtttcc ggcgggtcga tgatttcttc
     1321 gatttccgtc gggtcttccg gcggtgcttc cttggtaacg gtgacgccga attcatcggc
     1381 aacgagggag gccgtatcga aatccagttc ctggttgatt gtcgccatga tgccgcccat
     1441 gaggagcttc ttcatgactt cgccgacatc gcggcccagg cgttcggcaa aatccttgac
     1501 gatgattgtt tccggcagtt cgatagccgt cggataattc ttcttcttct tcggagcttc
     1561 ctgcgctacc ggtgcctgat gcttctggtt gcgcttgttc ttgttgtgct tgcctttgtt
     1621 caggacattg gacaacagcg tatggggacg gttgccgccg ttattcttgt tgttattgcg
     1681 gttattctgg ccgccgcgct tgttcttgtt gccactgcgg ttgtcattgc cgccctggga
     1741 atgaccaaaa tacttggtgc gcttgttgtc gccctgctgg ccctggctgc tctggccgcc
     1801 gttattgccg cctttatggt tctgcggacg gctgccctgg ttctggccct gagcggcttt
     1861 ggcaccgcgg ttcggctggt tctgcccatt gccctggttc tggcgtttct tgtcctgatt
     1921 ctggttattc tgtttcggcg tattgccctg gttctggcgt ttcttgtcct gatt
//
LOCUS       DAWABZ010000219         1937 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_11147_length_1937_cov_2.641870, whole genome shotgun sequence.
ACCESSION   DAWABZ010000219 DAWABZ010000000
VERSION     DAWABZ010000219.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11147_length_1937_cov_2.641870"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    620..629
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          524 a          450 c          526 g          427 t
ORIGIN      
        1 atacatatcc tttaccgcgc aggaacgttt ccgtttccat ttcgcgggtc ttgaagatgg
       61 cgaccggtgt gccggcctgg cggttataag aaatatacaa gtagtcaatg gaaatattgg
      121 cctggcggag gctgtcgagg attttgtcca gacagcccgg cgtatcgccc atttcgacgg
      181 cgacgacttt gtccatatga cattggtagc cggcttcctg caatttcgag acagccagtt
      241 ccggattggt gacgatgagg cggatgatgc cgaattctgc gctgtcattg gccagcatgg
      301 tgtagatatt gatattggca tcggctaaga ccgacgtcat cttgcgcaag gcgcccttcg
      361 tgttttcagt gaatacagaa acttgattga gcataaatcg aaatccccct tgatgataaa
      421 atattgtgac gttgtcccca ttttaccata aaaagacagg cttgtaatgg ggatgggatt
      481 tgttaaacgt ttcagaatgg gcaagtcatg atataataaa agcatgggaa ctatgttttt
      541 tacgatacga ggtgagggct atgccagctc gattggtcat cttcttattt ttactgctca
      601 caggcactgc caaactctan nnnnnnnnna aagccgtcat cgcggcgacc ctgacgacct
      661 ttgtcatcat ctgtggtctg cggcggagcg gccagaaaca ggccgaacaa aaagaacggg
      721 aaaagacagc ggcagctgaa aaagaaaaaa tccagtcggc ccaggaaccc gtaaagacga
      781 aacatcgact cgatgccgac gatgcggcct tcctgtcagc cgtcggcaca ggccttatcc
      841 tgcacgaact ccacgaaggg aacaaagaac aagaacgggt cgaacgcgat tacgacggga
      901 attgggataa ccccgatgac gacagccagt atgacgataa ccagtacgac gatagccaat
      961 atgatgactg ggagtgatgg gaatttcgca ggccgcagtt cgcagacggc agaccgcaaa
     1021 aaaatacacg gccaacggcc ttgtgcgtag gggctgtccc aagccttttt ggcccgtcag
     1081 gtgctgaccg tcgggaggcg caagacggct gccaaaacgg acagctgtgg ctgggcagcc
     1141 cgttgtgaga tttggcatac ctattcttgt atgatggtag gtttttacga gccacgagcc
     1201 acgaaccact attttttcac gagctgaggg ttccttgagg caatgtcgtt aatttgaatt
     1261 tatcgtcgcg cagcggcatt tgaatccgaa gtgaaccgcg gcctgcgaac tgcgggccgt
     1321 cagaccctaa attcgacgaa cgacctacga cgaacggctc tacgccgcgg cctgaggcct
     1381 tcatttcctg gagattccga aatatacgaa atcgccgaag aagcgaatcc gtgacatgac
     1441 gaaaatggag cgctggctgg cgtatttcgc taaccagctc ataccatcgt aggggtcgtc
     1501 cgtgggcgac ccgtcagcat gatagggctt gtcttgttca tgcatgtgga ttagaaggag
     1561 ggaactgatc atgagcgatg aagccatcca taaagccgta gatgccgccc ggacctttct
     1621 tcaggattac gccgaacgcc tggcttacat caaccgcgaa ctcgccatcc tagactataa
     1681 ctcggaccat cgcgatgcct ttgaagaagg ggaagctaag ggacgtgcag aaggaattga
     1741 aaaaggtcgt gcagaagaac ggaaatcgtc agataaccga tgggaaaaat tgatgggtct
     1801 gttattggag gaaaaacggt atgatgaagc gaaaaaagca gcttccagta aagagttccg
     1861 tgaaacactt ttcaaaaaat atggcttaga ataagacaga atttagaagt gaacgtactc
     1921 ccgcctatag aggcggg
//
LOCUS       DAWABZ010000220         1902 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_11338_length_1902_cov_2.195452, whole genome shotgun sequence.
ACCESSION   DAWABZ010000220 DAWABZ010000000
VERSION     DAWABZ010000220.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11338_length_1902_cov_2.195452"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1079..1106
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1601..1610
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          404 a          541 c          438 g          481 t
ORIGIN      
        1 gcactttgct ggcccgcttg cagttcggca aagctattga attgtatggc cgccgcaaat
       61 cgttatatgg cgggatcatc ctttattttg taacgacgtt attatatttt tatattccca
      121 atctcgctat tctctttgct atccgcttcc tcaacggcat ggcctatggc atcgtctcga
      181 cagcgacgaa cacgattgtc gcttcctgta ttcctgccga taagcggggt gaaggcatca
      241 actactacgg cctgagcact agcctggccg ctgccgtcgg acctttcctg ggcatgttcc
      301 tcatcgtcgt cacgaacttc acctttatca tcaccctctg cgccatcctc gtcggcttgt
      361 gcatgattgg ctgcatcgcc ctccacatcg acgaaattac ccttacgtct gaacaggtgg
      421 ccaagatgaa agccatgagc ctcgacaatt acgtcgaata tcgcgtcatg gtcatctcca
      481 tcatcggctt cttcatgggc ttcgcttatt ccagcgtcct gactttcctg gccgcttatg
      541 cccgtgaaat caacctggtc caggccggca cgttcttctt cgtcgtctat gccgtcgtca
      601 tcaccattac ccgtccgatg acgggcgtca tcttcgacca taaaggcgaa aattacgtcc
      661 tctatccctg ctacatctgc ctggccatcg gcttgttcct cctcagcatc accggtgctt
      721 cgtggcagct tctcttatcg ggcgtcttcg tcggcctcgg ctatggcacg ttcatgtcca
      781 acggtcaggc agtctgcatc aaactgactc cgtcgtaccg catcagcgtc gccctgtcga
      841 cgtacttcgt cgccctcgac cttggcatcg gcgtaggccc gtatgtcctc ggcagcctcc
      901 acggcctgct cacgttcccc cagctctatg ccgtcgctgc tatcgtagcc attgcctgct
      961 ttgccatgta ctacattttc tacggacgca agatcaacct catcaaccgg gccgtccgca
     1021 tctacatcca cgcataatac gtaattacaa tagatcctat ccccagattc gagggcttnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnaaca tcaagacgaa aatcctggcc ggcctggcct
     1141 gtatctgtct gatactgaca gtctttttct ggcctggcaa agaagaagat gcctatagtt
     1201 ccgacgtcgt cagggaagcg gaagacgtga gcgagcggga aacggaaatg ctggcacctg
     1261 atgaggtcct gcaggctccc gatggaaggg acatacgctt gcaggctctg tataaccaaa
     1321 agccggtctg cctctttttc tgggcatcct ggagcagcga cagcctgcgc cagctggcca
     1381 tcctggaaaa ggcctgtgtc cagtacggcc aggatgtcta tttcgtgacc atccccgtcg
     1441 gaccggatac agcagatagc cgggcctata tccaccagca gggatatgcc ctgcccgtat
     1501 acagcacaca gaaacagatg ctccatgact atggcgtcca tcaggtaccg cagcttatct
     1561 tcatcgcctg cggcggccag atgacgcgcc cctttaatac nnnnnnnnnn accgtattga
     1621 atgagcgcca ggtgacatac gaaatcaaaa aaatgctgaa ataattttcc catacccctt
     1681 gccaaatagg gaaaagtaag ttataataaa caagtcgcac agagaagtac aaaatatggg
     1741 cggttagctc agttggttag agtatctcgt tgacatcgag ggggtcgatg gttcgaatcc
     1801 attaccgccc accatattgt gtgtgaaaaa agagctgtct ttatagacag ctcttttttg
     1861 tggctagtgg ctagttgttc gtgggtaata cttagtgctt tt
//
LOCUS       DAWABZ010000221         1883 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_11456_length_1883_cov_3.441466, whole genome shotgun sequence.
ACCESSION   DAWABZ010000221 DAWABZ010000000
VERSION     DAWABZ010000221.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11456_length_1883_cov_3.441466"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1652..1661
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          417 a          541 c          505 g          410 t
ORIGIN      
        1 ggctagttgt tcgtggctaa tacttagtgc ttttacgagc cactagctac gaaccacgag
       61 ccatgagcca tgagccacga gccacgagcc acaaaccact atcatactct attcttaaaa
      121 gttattggta aaagtttcac aaatgtagta tcctgtacat gtgattcggg ttggacggaa
      181 tcttttggga tccgtaccgt aaaaaatccg ggtcaactat tatcataatt tcatagcata
      241 tcgcttggat cgaaagaccg ggacgaattc ttgtaaacgt aggcttttta cgcgcattcc
      301 cgagaggaat gcgctttttt ttgagggagt tatcatgcgt ttaggtatta tcggcgccgg
      361 cagagtcggc gcttcttttg tattagcttt tccttccgat gtcgaaggaa tcctttgttc
      421 cacagaagaa catacacgtc aaatggcccg ccgcctccat gctgctgcct ataccgacgg
      481 tgctgaactc gcacggcact gcgatgtcct cctgctcaca gtacgcgacg atgccctggc
      541 tcccgttagc cgggaattgg ccgcttccct gacaggggat gcgctgccgc ctcagacctg
      601 tatatttcac tgcagtggag ctatggattt atcgcctctt gagccccttt cacggttggg
      661 gtatcctgtc ggcagccttc atccgctgca aagctttgcc gaaccttcgg cagaccgcct
      721 gaagggaatc tacatggccg tcgatggtga tgaacgggcc aaggccgttg cggcagatct
      781 ggtccggact ttgggcagta cgcctttttt cgtaccccct gaagaccgca tgctctatca
      841 tgctgcggcc tgcttctgct ccaactacgt cgtgacagcc ctggccattg cccagaagct
      901 cataagccgc tggaccggca gtgccggcgc tgcatcccag gcgctgcggc ccctcgtcga
      961 agggacgatg gacaatgtcc gccagcatcc gctgtggcag acggctttga ccggccctgt
     1021 gtcccgcgga gatgccggga ccgttgccaa acacctggcc gtcatgccgg agaaatggct
     1081 ccagccctat tgtgcttttg gctgtgccgc tgcagactta gccttagaaa acgaaacgat
     1141 tacagagaaa cagcatgaca cgctgacaag aatccttgcg atggcggaag gagttcacca
     1201 tgaacaagaa agtaaccaat ctgaccatta agaaaatgaa acaggaaggc acgccgattt
     1261 ccatgattac ggcctatgac tacatcatgg cccagaacgt cgatgaagcc ggtatcgaca
     1321 tgatccttgt cggcgattcc ctgggcaatg tcgtcatggg tcatacgtcg acactgccgg
     1381 tgactatgga agatatgatc caccatacgg cggctgtcgt ccgcggcgtc aaacgggctc
     1441 tggtcgtcgg cgacatgccc ttcatgagct atcaggaaag cacccagcag gccctgcaca
     1501 atgccggccg gctcatgaaa gaaacgggct gcgatgccgt caagctcgaa ggtggcgcca
     1561 gtgtctgcga agccgtcaaa gccatgacga cggccggtat ccccgtcgtc ggccacatcg
     1621 gcctgacgcc gcagtccgtc catcagctcg gnnnnnnnnn nctcggcggc ttccgcgtcc
     1681 agggcaagaa cagcgaaacg gctaagcaac tgattgccga cgccttgaag ctggaagaag
     1741 ccggcgcctt cgccatcaca ttggaatgtg tcccgacaga tatcgccacg gaaatcacca
     1801 aagccctgca tacggcggct accatcggca tcggggctgg caacggcacc gatggccagg
     1861 tcctggtctg cacggacctc ctg
//
LOCUS       DAWABZ010000222         1875 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_11516_length_1875_cov_2.691209, whole genome shotgun sequence.
ACCESSION   DAWABZ010000222 DAWABZ010000000
VERSION     DAWABZ010000222.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11516_length_1875_cov_2.691209"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          514 a          468 c          457 g          436 t
ORIGIN      
        1 ggattattat gaagcctcat tttccaatct gggcctgacc ggaatcccca ttgctgatga
       61 atacccggaa aaatacgacc gccttttgtg cggcggtata tggtgcatcg tacagctgaa
      121 ttatgacgca gaaggaacgg agaacctgga tatcattctg cccaatgaaa acagccctca
      181 gtcaaataac cgaagacaga agaacatcta tcctattagt atccgcaatc tgacgcccat
      241 ccagatgcct catgttgaca tggatgaatt aaagcgcggc cgtcaggctt ttactaaaga
      301 agaatggatg gatgtcatgc tgcgctcgac cggtatggag ccggactctc tgtcatatcg
      361 tgaaaagtgg ctgctcctta cacgtatgat tccgcttgtg gaaaataatt tcaatctctg
      421 tgagctggga ccgcgcagta cagggaaatc acatatatac aaggaaattt caccgaacag
      481 tatcctcgtt tctggcggcc agacgacggt agccaacctc ttttacaaca tggccagcca
      541 tcagattgga ctggtcggcc tgtgggactg tgtggccttt gatgaagtag ccggcatcaa
      601 attcaaggac gaggatggta tccagatcat gaaagactat atggcatcgg gatcctttgc
      661 ccggggcaag gaagaaaaag cggcttcggc atcgatggtc tttgtcggca atatcaacca
      721 gtctgtcgat gtcctgctga aaacgtcgag cctgttcgac ccgttcccgc cagaaatggg
      781 aacggacacg gctttcctcg accgtattca tggctacaac cccggttggg aaatccctaa
      841 attccggcct gaacatttca ccgacgatta cggattcatt tctgattatc tggctggatt
      901 catgcgggaa ctgcgaaaag agcagtatgg tgacgccctg gatacctatt tccggctggg
      961 gaaaaatctg aaccagcgcg atactattgc ggtccgcaag atggtcggcg gatacatcaa
     1021 gttactgtac ccggatggcc gttttagcaa agacgaagta gaagaagtag tgcaaatggc
     1081 gctggagatg cgccgccgcg tcaaagaaca gctcaaaaag ctaggcggca tggaattcta
     1141 cgacgtgaat ttttcctaca tcgacaacga aacctttgaa gaacactatg tctccgtgcc
     1201 ggaacaaggc agtggcaaac tcattcctga aggcctctgc aatcccgggc aggtctatac
     1261 cgtttcccat gggaaaaaca acatgatcgg cgtattccgc ctggaaagcc agatgttgcc
     1321 gggtagcggg aaatttgaac gcaccggcat cggcagcgac agagaatgca aagaagccgt
     1381 caacactgcc tataactacc taaaagccaa tggcaggcgt atcagcggca gcatttcgac
     1441 gaccgccaaa gactatatca tcaactacca ggaccttcaa ggcatcggca tgacccatcg
     1501 cttagcgctc ccgacgctca tcgctatttg ctccatcgcc ctgaacaaac ccgtccaaag
     1561 ctccctcgtc gcccttggtg aaatcagtat cagcggtaca cttatgaaag tcgacgaact
     1621 ggccaacacc ttgcaagtat gcctcgactc gggcgcaaaa aaagtcctgc ttcccattac
     1681 atccgccgcc gatttgggaa cggtaccacc tgaattaatc ggaagttttc aactgatatt
     1741 ttatagtaat cctgaagatg ctgtctttaa agccttggga gtagaatgat gctatgcttc
     1801 aggtggattt agcaggattt acatgacgta tatcgaaact atactatata gtagaagcgt
     1861 gctgtacata ttttc
//
LOCUS       DAWABZ010000223         1862 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_11598_length_1862_cov_3.116215, whole genome shotgun sequence.
ACCESSION   DAWABZ010000223 DAWABZ010000000
VERSION     DAWABZ010000223.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11598_length_1862_cov_3.116215"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          450 a          515 c          517 g          380 t
ORIGIN      
        1 atatgggggg catcggcaag tcctaccggg gcatcgccga tgccgacctc atcagctgga
       61 accaggacca ggccagcatc atcatccagt ttcagcgcag tgatacgcag cagcagctga
      121 aaatcatctt atcccggacg gccaagaaag aactctgggt caatgaaaca aaagttcccc
      181 aaaaggaact ggtcgggacc ctcaacgaag tcctgttctc gccggacgac ttgcagctca
      241 tcaagggcag cccgtcgctg cggcgccgtt tcatcgacat ggaaatctcc caggtcagcc
      301 ggccgtacta ccgggaactg ctgcagtaca accgggccat ttcccagcgc aatctcctgc
      361 tgaagaagat gaaatatgaa ggcatccatc ctctcgatga atgggaccgc cagattgccg
      421 gctttgcagc ttcgctggtc gcgaaaaggc tcgaagccct gcacaagttg tcctttttgg
      481 ccggcgtcct ccacaagcgt ctgagccggg ggacggaaat gctgaaccta acatatgttc
      541 agccctatgg cggcggcgac cgggaccatc agaccaatcc ggaatggtac tatgccctct
      601 tgcagcgcca tctcgaagaa gacgtgcgcc gtcaggcgac atcggtcggg ccgcaccggg
      661 acgacctggt tttttccgtc ggcccggcgg atctgcggaa atacggctcc caggggcagc
      721 agcggacggc cgtcctggcc ttgaaattgt cggagctgga atacgtcaag tccgagacgg
      781 gtgaatatcc ggtcctgctc ctggacgatg tcatgagcga gctggacccg gaccgccggc
      841 aggccctgct cgatttcgtc cgcgaccgta tccagacgtt cattacgacg acggaaccgg
      901 ggatctttgc cggcatggcc ggctgtcatc ccatttgcat tgaacaaggg agggtcctgt
      961 ctcatggagg aacgcagtct tgaattaaaa aaagccggtt tagccatacc gaaagtattg
     1021 gagaaaaacc ggctcctttt gccttatcac ttataccagg ccaaacggga ctggaaacag
     1081 attgctggcg aacagattgc caaatattca tatatattag attttaagga taaacaggtt
     1141 atcattgccg tcatgaaccc ggtatatatg aattatctgt ttatgtataa aaatgaaatc
     1201 atagaggaaa ttaattctta cgtagggcat cgggccattg ccgatgtccg ttttgtcaaa
     1261 aaggggaaaa aaccggtccg gccggtctat gagaccttgc agggcgaaag ggaagatgtc
     1321 tttcctaaag agactatcag ccaggtccgc ctcgatgacg atacggtagc ccgtatccgc
     1381 caggaaacgg cccatctggc agaagggctg cgggaaaaag tggtccagct ccgtttcgcc
     1441 caggccaagc ggaagaaggc ttaccagctg gaaggcttcg tttcctgtcc ctgttgcggc
     1501 cgctggatgg cgcctggcga aaggcagtgc ctcttttgcc gcagcgaggc ccggcatgcc
     1561 ttgaagcggc agatacgcgc ttacctcgac gacatgccct ggctgtcgtg ggaggccgtg
     1621 gccggctatc tgaagacgga cgtgacggcg gctgcggaag aacaggcgta caatgaagtc
     1681 cgccgcaatc tcatctatac ctatattgaa aaggtatatt acgaatacga tacggcagcc
     1741 gatgatttta ccctggccat gctcatcacc cgccgcgtcc ccggcgatat cccgccgaaa
     1801 ttcatcgaga acctcgttgc caaatacaga aagaaggacg accatgtatc tccgtctgag
     1861 cc
//
LOCUS       DAWABZ010000224         1836 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_11750_length_1836_cov_2.321168, whole genome shotgun sequence.
ACCESSION   DAWABZ010000224 DAWABZ010000000
VERSION     DAWABZ010000224.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11750_length_1836_cov_2.321168"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          376 a          570 c          529 g          361 t
ORIGIN      
        1 ctgaacatcc agaaaagagg ctgtcccatt aagacagcct ctttttgagt attaagtttg
       61 cagtttgtag ttttttagtg cgacagcccc atacaaagga ggtgtccttt tgattgctga
      121 aatcatcatc aatacgacgg cgaaacggct gcagcagact ttttcctata ccgttcccga
      181 tggtctggcc gtccgcgtcg gcagccgcgt cctcgtgccc tttggtcatc gccgggaaga
      241 gggcatcgtc gtcgccctcc atgaacagct ggacgaaccg gccgatttca cactgaagcc
      301 cgtcgaaggc ctgctttcga cgcagaccgg cttccaggaa gaaatgatcc gcacggccat
      361 ctggatccgc gactactatg tctgcaacct ttccgaagcc ctgcgcctgt tcatgatcga
      421 caagaagggc ctggtccgcc aggaaatctt gtccgtcggc ccggctgcgc ctgtgagtcc
      481 tgaagggcgg cagctccagg cctatgtcgc cgacaagggg agccgtgaaa agggcagcct
      541 tgtccgccgc ttcggcaggg acctcgtcga aacggccctg gcccgccagg tcctgaccag
      601 ccggacttat tacaagaacc agattgccga caagatggaa gactggcttt ccttcgtcca
      661 cgatgatgac gaagacatcc tccacggccg gcggcgccag caggaactgc tccaggcctt
      721 gaaagagaag ggggaagggg ccgtttccta ttggcagggc cagggctaca gccgcgacct
      781 catgcgccgt ctctgtgcga caggcctggc ccgctggagc cagcggccgg ccaggacgac
      841 gaatatcctg ccgacccttt ctggcgatgc tccctttacg ctgacaccgg aacaacgcca
      901 ggcagttaaa gttatcggaa gcgatgaaca aggaaaaacc tacgtcctcc acggcatcac
      961 cggcagtggc aagaccgaag tctacatgca gctggccagc caggtcctga aagaaggccg
     1021 gcaggtcgtc gttttagtgc cggagatcgc cctgaccggg caaatcgtcc gccgtttcct
     1081 gcgccgcttc ggcgacgatg tcgtcgtcat gcacagccag ctttcccagg gtgagaagcg
     1141 taacaactgg ctgcgtatgc agaacgggac gagtcatatc tgtatcggtg cccggtcggc
     1201 tgtctttacg gcggcccagt ccatcggcct gttcatcgtc gacgaagccc atgacagttc
     1261 gtataagcag gacgaagcgc cgcgctatca tgccgtcgcc gtggcccgcc agcgggcggc
     1321 gtactacggc tgccccgtaa ttcttggcag tgccacgccg gcgctgggcg actactataa
     1381 ggccctgcag ggcgattatg tcctggtcga gctgacagag cgcgtcggcc agcggcccct
     1441 gccttcggtc caggtcgtcg acatgcgcga tgaattggcc cgcgggaatt accgcgtcgt
     1501 ttccgattgc ttattgaatc tcctggaaga gacactggca gcccggcaac aggccatcat
     1561 ccttctcaac cgccgcggct tctcgacatt cgttatgtgc cgcaagtgcg gctacgtcgt
     1621 caaatgcgat acgtgcgacg tggccatggt ctaccatcgt caggccgccc acctgcgctg
     1681 ccattactgc gatgccgaaa agcccgtgcc gacggtctgt ccgtcatgtg gcagcaaata
     1741 catcaaattc tttggcagcg gcacggaaaa agtggaacag cagctccacg aactcctgcc
     1801 gtcggcgcgc atcatccgcc tcgaccagga tacgac
//
LOCUS       DAWABZ010000225         1832 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_11770_length_1832_cov_3.753517, whole genome shotgun sequence.
ACCESSION   DAWABZ010000225 DAWABZ010000000
VERSION     DAWABZ010000225.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11770_length_1832_cov_3.753517"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          349 a          583 c          509 g          391 t
ORIGIN      
        1 atcggccgta tcctgatttt gtccatcgtc ctggccgttc tcttctatgc cctcatcatc
       61 ttcggtgtcg gctatgtcat gaataattcg gaaatcgccg ttgccatgaa aggcgtcggc
      121 ggcctggtca cggccgatgc catggctgtc gcttttggca gctccatcat ggccaaggtc
      181 ctcatcatcg gcggcctctg cggtatcatc accagctgga actccttcct catcggcggc
      241 agccgtgcca tgtattccat ggctgaatcg tacatgattc cgcacgtctt cgctaaattg
      301 cacgacaaat ataagacacc gatcaacgcg ctcctgctca tcggcctctt gtccatcatc
      361 gcgcccttct tcggcaagaa gatgctggtc tgggtcgtcg atgccggtaa cttcggctgc
      421 tgcgtcgcct actgcatggt cgccatttcc ttcgtcatcc tgcgcaaaaa ggctccggaa
      481 atggcccgtc cctacaaggt cagccattat aaactggtcg gcgtcatggc catcctcatg
      541 tcgggcttca tggtcgtcat gtacatgatc cccggctccg gcgctaccct ggttccccag
      601 gaatggggca tggtcctggg ctggtccgtc ctgggcgtcc tcttcggctt ctactgcaag
      661 gctaaatacg gcagtaaatt cgctacccac atcgacgtcg ccgtcgataa tgaagccgtc
      721 gaagaagcgg gcgacctggc tttcggtcag gccatggccg ctgccctgca cgacgtcgaa
      781 tccgatgcgc ctgtcaccta tgtatcgcag ccgctgagct tctccttctc catgcctgtg
      841 tccgtcgtct tcggtgccgg taagcgccat atggccgggg aactctttag ggcctatggg
      901 accaaggccc tggtcgtcac cgatgccgct accaatgggc gggacatcgt caatgaagtc
      961 gaaaagagcc tgaaagaggc tggcatttcc gtatcccgct ttgctcatat tacggggaaa
     1021 tccctggata ttacggctga ttcggcagtc gacgaaatca agaagaacgg cagcgacttc
     1081 atcgtcgcca tcggcggcaa taccgtcatg gaaggggcca agctgattgc cagtgccgct
     1141 gcccgcacct tgggcagggg catgatgccc ttgaatttcg taccgacgac gtatcacgcc
     1201 ggcgcctgct tcgatgcatc cctgcccgat ggcagccgcg acaacgccct gctggccaag
     1261 gtcgtcctgg tcgatccgga ctgcatgacc ggcatgagcc agaaagaccg ggctgtctcc
     1321 ggcttcgccg ccctgtgcga atgtatccgg gcctatacgg cgcccgatgc ccagccgctg
     1381 actgatgcca tggccctcta tgccatcgac atcctgaatg acaagctcgt cggcgtctgc
     1441 ggggaaaaca cagacaaagg ggcttgggaa aaagtcgtcc tggccggcgt catcggcagc
     1501 atggtcagtg ccgaaacggg ctgccaggtc gccaaagacc tgaaacaggc cgtcgccaag
     1561 acgtcttttg cagccgatga agtcctcctg gccgccatcg tgcccgtcgt cgtcgacgcc
     1621 aaggccggta aggatccctt cctctatggc aaactggccc gcaccatggg cggtttcacg
     1681 tccgatgact gcgctgcccg cctctgctac ctggccaagg ccctgcacct cgacctgcgc
     1741 attgccgaca tgggcgtcca tgaagacgac atcgccaacc tggccgaagc ctacatgggc
     1801 gaagaagcgg ccgaagccgt ccgggaaaaa gc
//
LOCUS       DAWABZ010000226         1811 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_11914_length_1811_cov_2.795558, whole genome shotgun sequence.
ACCESSION   DAWABZ010000226 DAWABZ010000000
VERSION     DAWABZ010000226.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11914_length_1811_cov_2.795558"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          361 a          442 c          571 g          437 t
ORIGIN      
        1 tgctggtcgt gaccagggtg atacccaggg gaatgccgat gataccgcca atgaccgtgg
       61 cgacggcgac catgtagaac gtttcttcaa tgccttggag cattaactta atgatttctt
      121 gggacataac ctaaaacctc acttccaatc ggtaatgttt tgatgtacga gaatgccttg
      181 gcctgtgttt ccatatcgcc catgatgacg acgatgaggt agccgaacgg ttcatcctgg
      241 atgtagtcga tgttgccgta gaggacgctg acttcgacgt tgaatttgcg gacgatgtcg
      301 gcgacgacgg ggtcgctggt cgcagcgcct ttgaagcgca ggcggacgag catgtagcgg
      361 tccggcgacg gctggtcggt gacacccagg ttcttgatgc ctgccggcag ttccatattg
      421 atgatggacg tgacgaaatc tttcatggtc tgggactgag gattggtgaa gatgtcgacg
      481 acgcggccca tttccttgat gacaccgctt tcgatgacgg cgacgcgatg gcagatttcc
      541 ttgacgacgg ccatttcatg ggtgatgacg acgatagtga tgcccaggcg tttattgatg
      601 tcctggagca gttccaggat ggatttcgtc gtctgcgggt ccagggccga cgtggcttcg
      661 tcgcagagca ggattttcgg atcgctggcc agggcacggg caatgccgac gcgctgtttc
      721 tggccgcctg agagctggga cggatagttg ccggcccggt cgctgaggcc gaccaggtcg
      781 aggagggaag cgactttctt ttcgatgacg tctttggaag cgccgacgag ttccagcggg
      841 aaggcaatgt tttcggcaac cgtacgggat gataagagat tgaagtgctg gaaaatcatg
      901 ccgatgttct tgcgttctgc ccgcagctgg gattcattca tcttggtcaa atccttgtcg
      961 tcgatgatga cctggccgct cgtcggtttt tctaacatat tgatgcagcg gaccagcgtc
     1021 gatttgccgg caccgctctt gccgatgata ccgaagatct cgccgtcgtc gatggtcagg
     1081 ttgatgcctt tcagggcctg gacctgtgcg gcgccgccgt aggtcttggt aatatttttc
     1141 agtgtaatca cagtatcaac tcctcaaaat aggcgtaact agagaaagca accacaaaaa
     1201 aaggccgtca tgcagatgac ggccgtatat acggtttcat ttcatctgtc ggaatgttcc
     1261 gttggaattg gcaccttccc gatagggggt tgccgcagcg tcatcgggcc agtccctcag
     1321 ctgctctgga tgaagggtat atggttgttg cgtcttataa tagcacggat ggccgggatt
     1381 gtcaatggaa aatcgttatt gcgcccagtc gccatcggta aagaccggca ccgtcgtgcc
     1441 gtcggccctg gtcccggtga tggtcaggtc cggcgagccg accatgaagt cgacgtggat
     1501 catggagtcg ttgaggccgg ctgcggcgac agcatctttg tccatatcag caccgccagc
     1561 caggcaggtc gggtacgatg cgcccagggc caggtggcac gaggcgtttt cgtcgaagag
     1621 tgtttcatag aagaggatac ccgacaggga aatgggcgaa tgatagggga tgagggctac
     1681 ttcgccgagg cggcaggcgc cgtcgtcgat ggcgatgagt ttcttcagga cgtcttcgcc
     1741 cgtttcggcg gcggcgtcga tgcctttgcc gtctttgaag gtcaggtgga atttgtcgat
     1801 gaggttgccg t
//
LOCUS       DAWABZ010000227         1799 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_11988_length_1799_cov_3.854931, whole genome shotgun sequence.
ACCESSION   DAWABZ010000227 DAWABZ010000000
VERSION     DAWABZ010000227.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11988_length_1799_cov_3.854931"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          453 a          558 c          469 g          319 t
ORIGIN      
        1 ggctgctgcc ggttacatga agggcggcaa ctacaacatg ttcaacccga aacagtatgt
       61 cacccgcggt gaagccgtca acgttctcta ccgcatcctg accggcgtca aggaaacgac
      121 gcagagccag gacggcctgg aatccaaggc cttcaaggat atcaaagacg tctacggctc
      181 aatcaaagcc tttgcatctg acggcatcat gtactggcag ggcgacaagc tccacatcgg
      241 cgtcaaagac ccgaagaaca aacagaaact ggccgatgcc attgctgccg acaaggatat
      301 cccggccgaa tcggtctatg tccagaaatc gacgtacagc tatgacgact ataagaacct
      361 catggcccag gccgaaaaga tttacaaggc tacggaagcg acgaatgcta ccgtttcgac
      421 ggaaccggat tacctcaatg aaaaagtcgt cctcacagtc agctccattt ccaaagaaac
      481 acagaacaac ctcaataaag ccctgggcag tgcccttcgc atcgtcattc agtaatcgca
      541 gcagaggagg atttccatgg tacggaataa aatcgctgcc atcctggcag ctgtggccat
      601 cttgtccgtc agtgccgttt cggccgatgc cccttataca gacctgggca gcctgaccga
      661 ccgcgcggct gtcgattacc tctatgatac gcagtgcctg accttcatca caggggatac
      721 ctttgagcca aaccgggtcc tgacccgcgg cgacctggcc cagctgctct ataattcggc
      781 agccaatatc cccctggcac cggtggaagc ggccggtgcc cacgatgtgc cgtcgggcac
      841 ggctggtgat gccatgaaca gcgtcgccgc ccagggtatc ctggcaggct atcaggatgg
      901 gaatttccat cccgaagaac cggtgagccg cgaagaattt gccgacgtca tctaccgcta
      961 cttgcagtac aatcagctgg ccgacccgga ccaggccgtc aagccctatg ccgatgccgc
     1021 gtcgattgcg ccggcttacg ccagggccat cgacgtcctg cacagcaaga acatcatggt
     1081 gccggcagat aactatttcc atcccaaaga aggcatgacc cgggccgatg ccgcccaggt
     1141 attttaccgc ctcatgcact ccgacgggga ctatacgtct catgtccagg tcgaatcgca
     1201 gctcatcaag gccatcaatg ccgaatacgg ctcagtgccg atctacttcc gcagcggtac
     1261 gatgtactgg gatggcgata ccctggtcct gggcatcaaa ggcgctccca gcaaatactt
     1321 gcagcagcgc atcaacacgg acgtagccaa gccgcaggcc gtcaagatac gtcgcgtatc
     1381 cttgagccac agcgattaca gccagctcat gaataaggcc atccattgcg tcgtcgacaa
     1441 cgaaggcgtc cagaactacg tcggcgccct gccggactac gtcaatgaac aagtcgtcct
     1501 cactgtccgc catcccgtca gcaaggcgac gctggctgaa ctggccaaac gcgtcggcgc
     1561 cggccgggtc cgtctggaaa cggctcctat cgccgggaaa gctcccatcg tccaggtagc
     1621 cggacagaca gacacagcgg aagaagcccc tgtgacgaca gaaaacacga aaaaagaaac
     1681 gaaacaactc tattccaaac tcttagacga cgccacatcg agcgccatca cctccgtcca
     1741 gaacgacgtg atgaagtgat ttgtagggga cgcccaaagg gcgtcccgca ggtcgcagt
//
LOCUS       DAWABZ010000228         1768 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_12190_length_1768_cov_3.107998, whole genome shotgun sequence.
ACCESSION   DAWABZ010000228 DAWABZ010000000
VERSION     DAWABZ010000228.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12190_length_1768_cov_3.107998"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1113..1122
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          457 a          493 c          406 g          402 t
ORIGIN      
        1 gtattaagtt tgcagtttgt agcaaatatt ttcaaattta aagccctctt ctacaaacta
       61 caaactccgt actacaaact ttatttccaa aattcttcgg ctttagcgat gttggcgcga
      121 atcgtagcgc agcaggcttt gaagtccttc atttcctgtt ccggcaggtt atattcgatg
      181 acttcttcga tgccattggc gccgatgacc gacgggacac cgcagaagat gcccgtttcg
      241 ccgtattcac catctaaagg cgcgctggct gccatgattt tccgttcatc ccgcaggacg
      301 gcccggacca tggtagctgc cgtcatactg atgccgtact ccgtgcagtg cttgccagaa
      361 taggtgaccc agccggcatg gatggtttca tcctggacgc gggccttatc gaagacatag
      421 gccggattct gttccagttc tgccagggat ttaccgccga acttgatgag cgaccagggg
      481 acgatctggg aattgccgtg ttcccccatc atgtaggctg agaaggaatg atgatccaag
      541 ccggtctgct gggaaatggc actgaccagg cgcgcgctgt ccagccccgt gccggtaccg
      601 aaaacatgac ctttcggaag gccgctcttc ttagcgatga ggtccgtaat gacgtcacaa
      661 gggttggtga tattgatgat gatgccctgg aagccgccgg ccatgacctt ggggatataa
      721 tcagcgacct gaggcacagt gaaatgcatt tcatcgtcgc ggttccccga tgcgcagagc
      781 gtaatgtcgc cgatagcatt gacgatgacg tcgcagtctg ccaattcttc atagctggca
      841 atcgtgtaat tgacacgatg aggcatgaac atgacggcat ccatcatatc ctgccgttca
      901 ctgaccagtt tttccgactt cacgtcgcat aacttgactt catcggcaat cccgaggacc
      961 cctaaattaa aagctacatg agccccgaca tgacctaagc cgatgacccc taccacacgt
     1021 ttcttaaaag ccatacaaaa cgcctcctgt tcactatagc atatagaaaa agtctattta
     1081 tatattatat caaaaaaaca ccctctttgt ccnnnnnnnn nnactttata caaatccaaa
     1141 aaaatccggg cctataatgc agtatgtaaa aatgtcaact acggtacagg aggccaggaa
     1201 tcatgacaag cccaagccgt ccaaagcccc cgctgggacg ctacggtccg ccagggaatc
     1261 aacgacttca tcgcggctta cggcaaagat tcgccgaact acgataaaaa caatcatccc
     1321 tatgccgtct tcgatttcga caatacgaca tccatcctcg acgtaggcga acagttggaa
     1381 gtctggcagc tcgatcatct gcaattcgcc gtcccgccga cacagatgaa gtccgtcctc
     1441 atgatgggga tcccagctga taaattagac gccatccgcg cagccgtttc ctatttcaaa
     1501 gtccctggcg tcaaagatgt cctggccatg accaacaaag tagacgccaa tggccgttac
     1561 ctcaatcagt acgattacga cctccatccc cagacccagg gcgtcggtaa agccgaaaca
     1621 ctggcaaaaa tcctggtacc gaaatacaaa ggccacggcc ccagcttcac ggctatggat
     1681 tcccaggggg atttcaactt ctgcaccgaa ttcaaagaca cgaaagaagt cttggtcatc
     1741 aaccggacgc gcaccgatga cgctgccc
//
LOCUS       DAWABZ010000229         1752 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_12293_length_1752_cov_2.613435, whole genome shotgun sequence.
ACCESSION   DAWABZ010000229 DAWABZ010000000
VERSION     DAWABZ010000229.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12293_length_1752_cov_2.613435"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          450 a          445 c          470 g          387 t
ORIGIN      
        1 actcaaactg acaacgacgc ttcgggaagc ccttagcgcg tagcgggccg cccgctgttg
       61 ctatccgttc tgtccatcgc ttgtactcga aggtcaaaaa gaatttgggc ggaccctacg
      121 gatacggcta cgctgcaaca aattcattat tgtattacga ccgtatgcca ttagttataa
      181 attccggctg catacaggat tcgtgcggga cgtcgctacg gccgtcaaaa cagacagcag
      241 tggttgggta gcccgttgcg aacagtcgac tatagtctat ttggaccatg tgacatggtt
      301 tggcgaatgg cgaactgcgt cctgcgaccg gcgggggcgt cagctcccaa actaaaatag
      361 gccgcatcta ccatcgggac gaaagcctcg tccggcggac ggccaaacgg ggcctggcaa
      421 aattgcgcgt caggctggct gaattgaggg actgacaaaa acacggagct gtcgcatgtg
      481 cgacagctct tttttgtgct actcttaggg aaaattgccc tgtcatggct atcatggtac
      541 aatcaatgta taagaataat catgagtatt atgtcatata caatacatag ccgctggaaa
      601 gatggcgaga tggaggaaat ggaatggata atcaggaata taattcgatt gtcagtttta
      661 tatggggcat cgcagacgac tgcctgcgag atgttatacg tgcggggcaa gtaccgggac
      721 gttatcctgc ccatgacggt catccgccgt ctcgacgcta tgttggaaga aacgaaggaa
      781 accgtgctgt ctatgaaaaa gcagctggat gcagcacaca tcgacaacca gtggccggca
      841 ctgtgcaatg cagctggcca ggctttttgt aacgcttcgc cgttcctgtt gaaggacctg
      901 accagccggg gtaagaagca gaccttggaa gcagacttca aggcctatct cgacggtttc
      961 tcgccgaacg tccaggaaat cctggataaa ttcgagttcc gcaaccaaat ccggaccatg
     1021 gtcgatgctg atatcctggg ggcggtcatt gaaaaattca cgtcacccga tattaacttg
     1081 agccctaagc ccggaaagct tccaggcctg gataatcatg gcatggggac tattttcgag
     1141 gaactcatcc gccgtttcaa cgaagaaaac aacgatgggg aacactggac gccccgcgat
     1201 gtcgtcgaac tcatggccga tttagctttc taccccgtag aagaccagat taaagatgct
     1261 acatattcct gttatgatgg agcctgtggg actggcggga tgctcacagt ggcccaggcg
     1321 cggctcttga agctagctgc cagacgagga aaaaaagttt ctattcacct gttcggacag
     1381 gaaatcaatc cggaaaccta tgccatctgc aaggctgata tgctgctgaa aggggatggc
     1441 gaggaagcgg aacacattgc ctatggctcg accttgtctc tggacgggaa cccgtcgcgg
     1501 cagttcgact tcatgctctc caatccacct tatggcaaga gttggaagac cgacgccgac
     1561 aaactgggtg gcaaaaatga aatcctcgat acccgcttca atacctacct gccgggcggg
     1621 gacctgctga agatgattcc ccgcgccagc gacgggcagc tcttattcct cctcaataac
     1681 gtatccaaga tgaagaccga taccgaactg ggcagccgta tcatcgaagt ccataacggt
     1741 tcgtccctgt tc
//
LOCUS       DAWABZ010000230         1752 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_12291_length_1752_cov_2.795522, whole genome shotgun sequence.
ACCESSION   DAWABZ010000230 DAWABZ010000000
VERSION     DAWABZ010000230.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12291_length_1752_cov_2.795522"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    302..311
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          389 a          532 c          476 g          345 t
ORIGIN      
        1 cgacgcagaa gttcctcgat ggcgaagtcg acgaagtcta tgtcatttac acgcacttca
       61 agtcggccct gtcccaggat acgcgcatcg tcaagatgct gcccattacc cagaaggaag
      121 gccagcagga aggggggccg cgggaagaat acctcttcgc gccggatccg aagctcgtcc
      181 tcgacgcctt gctgccgcag tatctggatc tgttcattta caacagcctc cttcagtccg
      241 ctgccagcga actcggtgcc cgtatgacgg ccatgacgtc ggctacggac aacgcaggcg
      301 annnnnnnnn naaagtccgt caggctggta tcaccaacga attgacggaa attgtcagcg
      361 gcgccaacgc tttgcagtaa gccgcctggc gatcattctg aaagagagga gtacctcttc
      421 atgagtaata atattgggaa agtagtccag gtcatcggcc cggtcgtcga cgtgcggttt
      481 gcctcggaag cagatttgcc cgccatttac aacgccatcc atatcaccgg tggcgaaggc
      541 gaccagaaga tcgacctggt tgtagaagtc atgcagcact tgggcgacga cgtcgtccgc
      601 tgcgttgcca tggactcgac ggacggcctc gtccgcggca tggcagctga aaatacgggc
      661 ggtcccatca aggtccccgt cggcaaaggt gtcctgggcc gtatcttcaa cgtcctcggc
      721 cagactgtcg ataaagtcga cgctaaagtc gaagccgatg attactggcc tatccatcgt
      781 ccggtcccga aattccagga ccaggcgacg gaaacggaaa tctttgaaac ggggatcaaa
      841 gtcgtcgacc tcatctgccc ttatgccaaa ggcggcaaga tcggcctctt cggcggcgct
      901 ggtgtcggca agaccgtcct catcatggaa ctcatccaca acgtcgctac cggccacggc
      961 ggctattccg tcttcaccgg cgtcggcgaa cgtacgcgtg aaggcaatga cctttggaac
     1021 gaaatgaagg aatccggcgt catcgacaag acggccctcg tctacggcca gatgaacgaa
     1081 ccgcctggag cccgtatgcg cgtcggcctg accggtctga ccatggcaga atacttccgc
     1141 gataaagaac acaaagacgt attgctgttc atcgacaaca tcttccgttt catccaggcc
     1201 ggctccgaag tttcggctct gctcggccgt atcccgtcgg ccgttggcta ccagccgaca
     1261 ctgggtaccg acgtcggcgc cctgcaggaa cgcattacgt cgactaaaga cgggtccatc
     1321 acgtcggtcc aggctgtcta tgtcccggcc gatgacttga ctgacccggc tccggcagct
     1381 accttcgccc acttggatgc gacgaccgtc ctggcccgtg aaatcgccga actgggcatt
     1441 tacccggccg tcgatcccct cgattcgacg tcgcgtatcc tcgatcccca cgtcatcggc
     1501 gatgaccatt acaacatggc ccgtgccgtc caggaaattc tccagaagta caaagacctg
     1561 caggatatca tcgccatcct gggcatcgac gaactgtccg aagaagataa gctgaccgtt
     1621 gcccgtgccc gtaaggtaca gcgtttcctc agccagcctt tcgccgtcgc tgaacagttc
     1681 accggtcaga agggccgcta tgtgccgttg aaagaaacca tcgaaggctt caaggaaatc
     1741 ttgtccggta aa
//
LOCUS       DAWABZ010000231         1751 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_12300_length_1751_cov_2.880896, whole genome shotgun sequence.
ACCESSION   DAWABZ010000231 DAWABZ010000000
VERSION     DAWABZ010000231.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12300_length_1751_cov_2.880896"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          460 a          511 c          432 g          348 t
ORIGIN      
        1 gtttttcacg agaatatacc gcgggtcgcc cttgacggcg tactgctcct cgtcgatgct
       61 tttgaaggcc cgatgccgca gacgaaatac gttctgcgca aggctttgga acagaaattg
      121 aaaccgatcg tcgtcatcaa caagatcgac aaacccggtg cccgtgtcgc tgaagtcgaa
      181 gacgaagtac tcgaattgtt catggaactc gacgctacgg acgaacagct cgatttccct
      241 gtcgtctatg caaacggccg tgacggcatt gctaaatact ccatggatga cgacagcgac
      301 aacctcgaac cgttgttcaa gaccatcatc aaatactgcc cgtgcccgaa aggcgacgct
      361 gaaggcccgc tgcagttcat ggtcacgacc ttggactacg atgattatgt cggtaaaatc
      421 gctatcggcc gtattgtccg cggcaagatg tatccgaacc aggctgtcgt cgtcactgac
      481 ggcgaaagcc agcgcaaagc taagattggc cgcgtctata cctatgaagg cctgaaacgt
      541 gtcgaacagg atgaagccgc tatgggcgaa atcgcctgca tcgtcggcat cccggacctc
      601 aagatcggcg aaaccgttac ggacccgcag aacccggaag ctttgccgaa aatcaacatc
      661 gacgaaccga cgctgtccat gattttctac gtcaacgaca gcccctttgc cggccaggaa
      721 ggggaatacg tcaccagccg tcacctccgc gaccgcctgt tccgcgaagt ccagaccaac
      781 gtatccctgc gcgtcgaaga aaccgacagt gccgatgcct tcaaagtatc gggccgtggc
      841 gaactccaca tcgctgtcct catcgaagaa atgcgccgtc agggttatga acttcagatc
      901 ggcaaaccga gcgtcatcac caaggaaatc aacggccaga aatgcgaacc gctggaagcc
      961 ctcaccatcg acgttccgcc tgaattcatg ggcgctgtca tggaaaaact cggtacccgt
     1021 aaagccgaaa tggtcaacat gaccgatatt tccggctaca cccgcctgga attcatcatt
     1081 ccggcccgcg gcctcatcgg cttccgcagc gaattcctca ccgctaccaa gggcaatggc
     1141 atcatgtacc acgtcttcca cggctatgct ccctggaaag gcgaaatccc gggccgtacc
     1201 cgcggcagct tagttgcctt tgaatcgggc acgacgacaa gctatggcat cttcaacttg
     1261 caggaccgcg gcacgatgtt catcaaaccg ggtgaagaaa tctatcttgg ccaggtcgtc
     1321 ggcgaaagca accgcgacgt cgacatcgac gtcaacccgt gcaagaagaa acacttgacc
     1381 aacacccgtt cctccgcatc ggacgaagca ctccgcctca cgccgccgca ggaaatgggc
     1441 ttggaaaaat ccctggaatg gatcaacgac gacgaactcg tagaagttac gccgaagagc
     1501 atccgtatcc gtaaagccat cctcgacaaa cacgatcgta aaaaagcaat gcgccgtgcg
     1561 cagcagtaag ctttttacta aaaactataa actgcaaact taatactcaa aaagagggtc
     1621 tgtcgcacgt aatcgtgcac aggctctttt tctgtatgca aaaatatcat agctttatgg
     1681 tcatttttca gggactgttt gctcggactc agaagcggct ttgccgcaat agactctgga
     1741 aatcaggcgc a
//
LOCUS       DAWABZ010000232         1726 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_12459_length_1726_cov_3.128067, whole genome shotgun sequence.
ACCESSION   DAWABZ010000232 DAWABZ010000000
VERSION     DAWABZ010000232.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12459_length_1726_cov_3.128067"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          393 a          441 c          486 g          406 t
ORIGIN      
        1 gatatactca agatgtcttg agaaactgac tcataaaaat agattcatac gagaaaagga
       61 agcgatacta tgattactaa agacatgacg ttagctgacg ttgtaaaagc ttatccgaat
      121 acgattgggt tcctgaatgg tctgcatctc gattactgct gcggcggcca tgaccccatc
      181 gctacggctg tccgcgaaaa gggtctggat gtagatgaat tcctggccga gctcaatcag
      241 gccgcagaaa gaaaggcggc gcagcgggat gtacatggcg atattgaagc gttcaaaacg
      301 cttgcagtca cggaaatgct tgacgacctg gaagcgacgc atcacgttac agaccgcaga
      361 ctcatggctg aaacggaaaa acttctcaac aagatcctca tcgtccatta tccgcatcat
      421 ggtaaaatgc tcacgcgcct tcaccatctt tatgccggcc tgaaggcaga actggaagag
      481 catttcgcca aggaagaaca gctcgtcttc ccgctgatgc gccagcatcc gcatccggac
      541 agccagacgc tggcacttat ccaggacttg gaagcagaac acaccggcgc cggcgacgtc
      601 atcaaagaaa tccaggccct gacggataac ttcacgccgc cagaagatgc ttgcccgact
      661 ttccgccaga cgtatgttgt catggaacag ctctttgacg acatcttcat ccacatcttc
      721 aaggaaaact ccatcgcctt cccggaatac gccgaacagg tctgaatata aggaaaatca
      781 cctgcctcaa ggattgatca acatctttgg gacaggtgat tttttttaga aatttgttta
      841 ttttttcaaa tacgagtcga agaaagcggt gatgatgtcg aggacttctt tttggttcca
      901 gtagacgccg ccgtgggccg ctccttctac cagatagcgt ttcgagggga tgttatgttt
      961 ctggagggcc tggaagagca ggtccgtctg gctcggggag acgacggtgt ccttcgtgcc
     1021 gtgcatgagc agcataggcg cactggtttt ggaaatgtaa gtgatggggt tggccttttt
     1081 ggcgccttcc ggattggcca ggatgccgcc gtcctggccg ccgaatacgg cagagccgtt
     1141 cacccaaagg gcttccgtgg cgccaggcga ctggtgggct tttttcacgg attcactgta
     1201 gtcgtcgccg atgcgggtga ggtcagagag gccatagagg tctacggctg cttttacgtc
     1261 gctcccgacc tggagattat cgcctttgtc aaaggtcgtc gtgccgctgg tcgtcgcggc
     1321 catggccgtg aggtagccgc cggcactgcc accgatgatg ccgattcgtt ccgggtcgat
     1381 gccgaattgg tctgcatggg ctttcaaata gcggatagag gccttcacat cttccagggg
     1441 ttccgggaag cgggccgtag gtgccacgag gtactggatg gaagcgacga cgtagccggc
     1501 ttctgccagg cgcatgcgga gttgtatgcc gttgtcctta ttggcattga tgaagccgcc
     1561 gcccgtgacg taaacgatgg caggctgttt cgtgtccgat ttcggctgca ggatgtccat
     1621 tttcatggcc acgttggggt agccccgcat cgggacctgt tcgtatacga cgtcggaaat
     1681 ctgattgatc atcggcgtct ttaccggtac ggataacacc gtactt
//
LOCUS       DAWABZ010000233         1707 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_12582_length_1707_cov_2.934625, whole genome shotgun sequence.
ACCESSION   DAWABZ010000233 DAWABZ010000000
VERSION     DAWABZ010000233.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12582_length_1707_cov_2.934625"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          330 a          502 c          448 g          427 t
ORIGIN      
        1 cgatgatctg taaacccaaa tccgcccggc ggtcatcctt ccggatattc gccgcggcct
       61 gcccggctgt cgggtccggg tatccttcgt ggttcttcat aggcttctcc tttcacggac
      121 cggatgcggg ccttcaggct gttcaagaca aaggcctggg ccgcgtcctt cttcgtcagg
      181 gcttctgcta aatcttcatc gcgtgtgccc tcacaaatca aatggtgtac gatgaccggg
      241 tattcctgcc cttgccggtg caagcgctta ttggcctgct gatacaattc cagggaccag
      301 ttcagcccga accagatgac gtgatgcccg ccatgctgca agttcaggcc ataccctgtc
      361 gatgccggat gggccaacag caaatccaca tcgccattgt tccaggccag ttcgtcgtcg
      421 gcgttctcgt acacccggta ccgcagtttt gttttggcca gggcttccgt catccggtcg
      481 cggtcgtgct gaaaattgta gaagactaag gcatgctgcc cgttcagctt ttccaccagc
      541 tccataaagg cctccagctt gcagtcatgg accacatgta catcgtgctc atcgtcatag
      601 atagcaccat tggctaattg ctggagtttg ttcgacagtg ccgccgcaga tgtcacgtcg
      661 atatcgccat ccggcagggc aaggatcatc gtcttttcca attccttgta cgcctttttc
      721 gctttcggcg acaggaccac cggcacatcg tcataaatac attccggcag ttgtaagtag
      781 tcggacgctt tcatgctgat acagatgtca gcgattttct tcatgacagc atcctgggcc
      841 ccgtctttcg ggtcatagtc gtaaatcaca tcacggctgc ggcgccccgg ctcaaagtag
      901 cgctcccgga aatgggtata gtagcggccc agccgttcgc cgcggtccag cagatacacc
      961 tgactccaca ggtccatcag gccgttcggc gacggtgtcc ccgtcaggcc gatgatgcgg
     1021 cggatatggg gacggatggc cgccagggcc ttgaaccgtt tggcgctgtg gctcttgaac
     1081 gatgaaaatt catcgcatac caccatgtca aagggccagt cgttctgata gtagttgacc
     1141 agccagacga cgttttcccg gttgatgacg tagatgtccg ctggtgtatt cagcgcccgg
     1201 atccgtttct tttcgccgcc caggaccgtg gaaaagcgca ggtgcttcaa gttgtcccac
     1261 ttctgcgctt cccgctgcca cgtcgcctcg gctacctttt tcggcgcgat gacgagtacc
     1321 cggcggacct gaaagcggcc gtatttcagc tcattgacgg cggtcagcat gattgccgtc
     1381 ttgccaaggc ccatgtcaag gaaaagcccg atggccggtt tctgcaaaat ttgccggata
     1441 cagtactgct gatatggatg cggcttgaat atcattttgc ctccctccta atcgctacgg
     1501 tttcgattct acgcccaatc ggatggagtg gacgtctaaa tactcattga cggccttttc
     1561 gccccgcaga acgtaaacga tgcaattccg ctgccgcagg cgtttcattt gtgccaattg
     1621 cagcggcgtc ggtttgtttg tttccgtttt cagctcgaca aattcaatct tgccgtccgg
     1681 gaaaataacg attcggtccg gtacgcc
//
LOCUS       DAWABZ010000234         1689 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_12726_length_1689_cov_2.862301, whole genome shotgun sequence.
ACCESSION   DAWABZ010000234 DAWABZ010000000
VERSION     DAWABZ010000234.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12726_length_1689_cov_2.862301"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          315 a          471 c          500 g          403 t
ORIGIN      
        1 gcatagggag cggccccttc gagggtgctc atgcccttgt aagtcatgta gttgtaagcg
       61 atgacagcaa attcttcgcg ggtgatcttc tgttccggtt tgaacgtgcc gttgctgtaa
      121 ccgctcatga tctgtttgtc gaccagcgag gcgatggccc gttcggacca gcggcctttg
      181 acgtcgctga agttggtcgt cttgactttg gaatcttcac cgatcatgtt gttgatgatg
      241 gcagctgcgc cttcacgggt aatgtcttcg ttcggacgga actggccgtt cgtgccgctg
      301 atgtagtgct ggttataaaa atactggata gcgtctttgc cccaataatt gctgatatcc
      361 gtaatccccg tcgatgcggc aaaagccgac gtgccgaggg cggctgtcag ggccgtaagg
      421 accatcaatt ttttgtactt gttcatgatt cattcgctcc tatctatgtc agtgtcgtaa
      481 aggatacatg gattgcatac ctatgtttca tatcttattt attttatcac accccgccct
      541 attagggaag gcatagagcc gtgatttgca ggcctcaata ggcgtcgaag atggcctgta
      601 ttttttcccg gtccgtcgtg tgggccaggg ccagctgcaa gaggatcttg gccttctgcg
      661 gcgtcaggga atcggctgtg accgtcccgt cataagccgg gacctgggtg accatgccgc
      721 ccagggcgcg ggcagcccgg acgacgacca gcccctgttc catcaagggc tgcaaggcct
      781 gccagaccag ttcgggcatc ttgccatgac cgagacccgt gatgaccagt cccgctttcc
      841 cctgggccag gcggcgtacg tcgtcagcgg tgaggccgac ataggcatag acgatggcga
      901 cggctggcag ttctttagga gcggccaggt ccgacgacca cgtatgcttg cgcaggggct
      961 gctggtacag gcggggccgg ccgtcctgca tatagcccag gcagcccatc tggcggcctt
     1021 taaaagtatc ggcatgggtc gtatcggtct tttcgacgaa gcgggcgctg tggacggtgc
     1081 tgttcatgac gatgacgaca ccgtattttc ccaggtccgg ccaggacgcg gcctggaccg
     1141 cttccaggag gttcagcgga ccgtcggcac tgatggccgt cgccggccgc atggaacccg
     1201 taaggacgac gggcttgatg gtgtggaccg tgaggtcgag gaaatacgcc gtttcctcca
     1261 tcgtatccgt gccgtgagtg atgacgatac cgtccacgtc ttcccggtcg gctacttcct
     1321 ggacccgatt ggccagggac agccataaag cttcggtcat atccgagctg tcgatgttgc
     1381 agaactgttc gccctgcacg tcggcataat cagccagctg gggcacggct tccagcagtt
     1441 catctatcga cgtctgaccg gcagcatacc ccgtcaggtc atcggccgac gcggccttgc
     1501 cggcaatcgt cccgccacag gccagtacat atatcttctt tttttccatt ctcatagctc
     1561 ccctctgcaa tgatgatatg gttctacttt accacaaatg gggggttagt ggctagtagg
     1621 ggccgtcctg aagtctttcc agcccatcgg gtgccgacca acggaaggcg caagatggtt
     1681 gctggaacg
//
LOCUS       DAWABZ010000235         1623 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_13242_length_1623_cov_3.135842, whole genome shotgun sequence.
ACCESSION   DAWABZ010000235 DAWABZ010000000
VERSION     DAWABZ010000235.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13242_length_1623_cov_3.135842"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          364 a          466 c          465 g          328 t
ORIGIN      
        1 tgtttttgca tacataaaaa gagcctgtgc acgattacgt gcgacaggcc ctttttctat
       61 tgcattttcc gtcagacaag atataataaa aatattgtaa tagaatgtaa ccgtgaggag
      121 gaacgctggt tggcagaata tatcatgctg caggggacga gttcccatgt cggcaagagc
      181 atcattacga cggctttgtg ccgcattttc ttacaagaag ggaaacgggt cgtccctttc
      241 aaggcccaga acatggcttt gaattcctat gtcactgccg atggccggga aatgggccgg
      301 gcccaggtcg cccaggccga agcggccggc ctgctgccca tggtggccat gaatcccgtc
      361 ctgttgaagc ccacaggcaa tgcctgctca caggtcatca tcgacggtcg tcctgtaggc
      421 aacatgtcag cccgggaata tcataaaggc tatgcgctcc aggctttcga cgccgtcaaa
      481 cgggccctgg gcgaactgga tagggcttac gacacgctgg tcatcgaagg ggccggcagc
      541 ccggcagaaa tcaacctgaa ggaccatgat atcgtcaaca tgcgcgttgc caaatacctc
      601 ggcgcgcccg tactcttagt ggccgatatc gaccgcggcg gctcgctggc tgccttagtc
      661 ggcacgctgg aactgctcga cccggatgaa cgggacctgg tcaaaggcct ggtcatcaat
      721 aaattccgcg gtgacgtatc gctgttcacg ccggccatcg attttttgga aaaaaagacg
      781 ggcaaatccg tcctgggcgt cgtgccctat gtcgatgacc tgggcatcga tgaagaggat
      841 tctgtatccc tcgacgatag ggaacagcgg ccagaagctg ataaggtatc tatcgccgtc
      901 gtccaggtgc ctaagatttc caacttcacc gatttcgaca gcctggcccg cgaagacgac
      961 gtcgccctgt actatgcccg ccatccccgc gacctggccg gcgccgatgc cgtcatccta
     1021 ccgggaagca agaatacgac agaagacttg tgtttcctcc tggaacaagg ctgggaccgg
     1081 gctttacagc atttcatcag ccaagggaag cccgtcatcg gcatctgcgg cggttatcag
     1141 atgatgggcc aggccgtctg cgacccggat cagaccgagt cccaactggg ccgcgtcgaa
     1201 ggcctgcacc tcctgggagt gacgacgacc ttcgtatcgc ataaactgac gtcgcaggtg
     1261 acggctgact gccgcgacct gccttttgcc ggaaccatcc tgaaagcgaa gggcctgacg
     1321 ggctatgaaa tccatatggg ccggaccgag cgggatggca gcgatacgta tccctttgtc
     1381 gtcaccagcc gttctggaga accctgtgaa agtctggccg gtgccgcccg taaagatggc
     1441 ctggcctggg gaacttatat ccacggcatc tttgataacg acgcgttccg ccgccaattc
     1501 ctcaatgccc tgcggatccg caggggatgg cagcccatgc cggtgacgct gcactaccgg
     1561 caggaaaaag aagcgaaata cgaccgcctg gcccggatcg tccgggagtc tctggacatg
     1621 aag
//
LOCUS       DAWABZ010000236         1534 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_13995_length_1534_cov_2.695740, whole genome shotgun sequence.
ACCESSION   DAWABZ010000236 DAWABZ010000000
VERSION     DAWABZ010000236.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13995_length_1534_cov_2.695740"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          390 a          426 c          458 g          260 t
ORIGIN      
        1 gctacgcgct gagggcttcc cgaagcgtcg ttgtcagttt gagtctgtcg cggcggaacg
       61 ccgcttctga ttccgaaaaa aagccgcggc tcctctccct tctctcttct ttataggtaa
      121 cgcgttacag aggcacggga agcaaaaaaa aagcggctgt cgcattaagc caaaaatgca
      181 taatgcaaca gccgcttccg ggatatgtac gatttcccga cctacttgcc agaaaaattg
      241 tcccaattcc taacgggtcg cccacgtggc gccccctact atcctaaaaa tactctgcag
      301 attacaaacg ctatcctacc acgccgcagg cagccagccg cagaggctga ggatggcgta
      361 gacgccggca ctggcaaaga ggaggacgac cagggtctgc atgatgggat gggcgatcca
      421 gccgcattgc caacacggtt tcttgtcgcc gtgtttccgg gaactgcgca gcatgaagac
      481 gggcaggatg gacatgatga tgccgccgaa ggtgccggcg ctgaagatgg cgttgacaaa
      541 gccgacgagg ccgctgtagg ccaggaggac cggcgggatg acgatgaagg ccaggcaggc
      601 cgcccgcgtg cggatgtccg tttccgactt gaagcccagg cggtcgatca tgttggtcag
      661 gaaactttcg gcaatggccc agaatgacgt cagcatggcg cagaaggcaa aggcattgac
      721 gatgacgaag aagaccggcg accccagggc gcggccccag gataaggacg cgacttccga
      781 aatttcatcg acgggcagca tgaggaagac ggccagcggg accagggcca ggatgacgaa
      841 ggaaatgccg gccccggcca ggatggacgg gacgagtttc cccggttcgt gcgtgaagcc
      901 ccgggaaatt tccggtacga tgtactggac agcgtaacag aagacggcaa tgttgaagac
      961 cggcacggca tacatccagt gggtatagag gatatctgcg acaggtacgc gggccgaaaa
     1021 aagtgacgcg ccgacgagga gaataagcat ggccgtcata cctgaactca aatatttctc
     1081 tgctaccccg gttgccttga ggcccagcca gacgacgctc gtcgccccga gggcaaagag
     1141 gcagctggcc atttccaggg aaatgccgaa gaattcgctc aatatcttgc cgcagccgct
     1201 ggtataggcg atgaggctgc aaaacgacgt ggccccgacg gaaaagaaaa tcagccagga
     1261 accgatcttg ccgacgtact tttcagccag gcccgacagc tgtaagggcc tcgtggtgcg
     1321 cagcgtcgct tcggcgacat agagcatgga gatgatagaa aaaaagcctg ctacgacaag
     1381 ccacgtaagc aggacgggcc agccggcatt gcgactggca aaagctaaac ctaaaacacc
     1441 ggaacctatg gtagacccga cgaccatcat cgtagcctcc ataaacgtaa gacgatgtac
     1501 cttgagagcc gcctcttttt tcgcagctcc cgcc
//
LOCUS       DAWABZ010000237         1500 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_14317_length_1500_cov_2.800692, whole genome shotgun sequence.
ACCESSION   DAWABZ010000237 DAWABZ010000000
VERSION     DAWABZ010000237.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14317_length_1500_cov_2.800692"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          314 a          466 c          450 g          270 t
ORIGIN      
        1 ggtgcgccgg ggctggcaca tcgacagcca ggtccagaag gatgataaat cggaagccaa
       61 gttccacctg gcccggaaag gggacgatac gctcctggcc gtgaccggaa cggccagcct
      121 ggccgacatc aagtccgatt tgaacctcca ttccgtcgcc tacggcggca cggccgggaa
      181 gaccgctaga aaggacggcg tccgccgcgt ccatgccggc tttgaccact acacgacgac
      241 ccttctggca gcgccctatg gcggccagac cgtaggccag gtgctggctg ccgatgccca
      301 tgcaccgaaa cggctgacct tgaccggtca cagcctgggc ggagccgtcg ccgtcctggc
      361 ggcggcccgc ctggacgacc agggtgcgtc gcagctccag gtcgtgacct tcggcgctcc
      421 ggctgtcggc aatgacgcct ttaatgaggc ttacggccgg cgcatccggc tggaccgcat
      481 cgtcatggag ggcgatcccg tagaaaaggc cgtacaggcc gtttcccgca cgtatgaaca
      541 gttcccggac aagacggtct ggcaggcagc gccgacgacg cgccgctttg cccatgatat
      601 agccggatat gccgacaccg ctctgcgccg ttattacgat gctaagacgg cttatgaaac
      661 ctatctgggc catgctgtgc cagacgatgg cggccggcct ttcggctcac cggtctgggt
      721 cccgcccttg tccctgaccc tcgacgaggc cctgcaggcc gatacgccct atctggaacg
      781 ggctgccgac gaccagctcg gctaccgcct gcatcctgtc tttgcccgca gggaacagtc
      841 cctgggagaa gccctgcgcc aggcccggca gaacggctgc agcgaggtcc tgctccgcca
      901 gttccaggcg cggcgcctca agggaaaaga atatgacttt gccctcacgt atgacgaaat
      961 atggtacgat gcccggaccg gcctggtgcg gacggccgtg tcccgcacga ccgatacggg
     1021 caagatgaca cccattgaag ccctgctggc tctggccggt gccgactagg cgcttgtact
     1081 cctgtcaggc ttgtaatata atgaaataga aatatataca gaaatatacg gatggtatat
     1141 acgaaatata aaatatatta cgacgggaga tggacgcatg gaacatcaaa tgaacgggaa
     1201 ggaccaggcc gctttgacgg cctgcatccc ccagagcctg gccgggggaa tctttattta
     1261 taaagccgat gaaggggaaa cactgctcta tgccagtgac tatgtcatcg gcttttttgg
     1321 ctgcgcttct tacgaagaac tggccgacta cgtccatcac agcttccgcg gcatggtcga
     1381 cctgcgggac cgggaactgg tagaacagaa tatccgccag cagacggcga gcgacgtctg
     1441 ccagaccagt tatgcccgct accgcgtcat cgcctgtgac ggtaccgtac gctatgcaga
//
LOCUS       DAWABZ010000238         1500 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera sp. isolate gSpreadC_ID_575
            NODE_14315_length_1500_cov_4.281661, whole genome shotgun sequence.
ACCESSION   DAWABZ010000238 DAWABZ010000000
VERSION     DAWABZ010000238.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993075
            Sequence Read Archive: SRR3737001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera sp. (human gut metagenome)
  ORGANISM  Megasphaera sp.
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Megasphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14315_length_1500_cov_4.281661"
                     /isolate="gSpreadC_ID_575"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2023260"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          345 a          417 c          400 g          338 t
ORIGIN      
        1 gctacgcgct gagggcttcc cgaagcgtcg ttgtcagttt gagtctgtcg cggcgaagcc
       61 gcatctgagt ccgaggcggc ctgcggcctg cgaactgcga cctgcttttt ccttcatgct
      121 ataataaggt taccatgctg agagaggagt catcatcatg aatgaaaaga ttgaacaata
      181 cgtacggaat cacggccgtg attttatgga atggctttgt gatattattt ctattccttc
      241 accgaccgga catgaacagg ccaagggcga atggatattg aatcttttgc atcaatgggg
      301 cgccgccggg gcgtatcgtg atgccgcagg aaatgtggtc tatccctgcc atgtaaaaag
      361 cggtgaaaag gtcgcccttt acaccgctca tatagatacg gtattccagg atttgcagga
      421 aatccacatc cgccagacag gtcacatttt gtcagcgccg tcgtgcagcg acaacagcgc
      481 cagtatcgcc ggtctattat ttatcatcaa aatgttccac gatttacagc tcacgccgcc
      541 gcaaggtctg ctctttgcct tcgacgtcgg cgaagagggc ttgggcaatc tcaagggaat
      601 gcggcaggtc atggccgatt ggcacggccg cattgccgaa gtggtcgccg tcgattgtac
      661 tttcgatact tttgtgacga cagctgttgg ctcccgccgc tatgccgtca ccgtcgaagc
      721 cgccggcggc cacagctgga tgcatttcgg ccagtccaat gccattgccg aagcggcccg
      781 catcatcagc cagctctatg cactgtccgt gccatcccag ccaaagacga cgtacaatgt
      841 cggcaccatc acgggcggca cgacggtcaa ttccattgcg gcaaaggccg tgttcactgt
      901 cgacttgcgc tcggaaaatg ctgatgaact ggacaagctg gaccaaactt ttcaaagcct
      961 cctggcccat actgaagggg aaacggtcca catcaaaacc cagctgctcg gcgaacggcc
     1021 ctgcagtcgc ctggacacca atcccctggc agaccgcatc acggccatcc ggcggcgcca
     1081 cggcctgccg acgccgtgca ttgccggcag caccgatgcc aacattccca tgggccaggg
     1141 tatcccggcc atttccttcg gtttctgccg cagccacggg gagcataccc ttcaggaatc
     1201 gctggacgtc gatacctttg tgcccggcat gatccagatg ttggaattca tgggagtgta
     1261 aaaaaaaggc ttgtcgcata tgcgacaagc ccttttatcg taggggccgt cccaaagggc
     1321 ggtccgttgt ggctagttgt tagtgattag caaatggtaa aaaatttaaa gccatcttct
     1381 acaaactccg tactacaaac tataaagttt aaggaacctc gcataatggt agaagccttc
     1441 gtgcgacagc ctttttttca gtaggggcga caacgtgggg cgaccgctcc aggatatata
//
