LOCUS       DAWACS010000001        13565 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_553_length_13565_cov_2.760326, whole genome shotgun sequence.
ACCESSION   DAWACS010000001 DAWACS010000000
VERSION     DAWACS010000001.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 13565)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13565)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13565
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_553_length_13565_cov_2.760326"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3058..3067
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3494..3503
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10521..10530
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12649..12658
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13204..13213
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4451 a         2525 c         3164 g         3375 t
ORIGIN      
        1 cagagtttta cacctaattg gggataaaac agcttatggc gggcggcggc gtcgtgctga
       61 ttggaactac acttgttccc ttgctctcag ggctgtttag ctgatgaact caataattga
      121 taaaattaca caagcgattt gcgattttct gatttcgctt attgagggta gtttatcaac
      181 aatgtttaat gacgtaaacg agaaagtcgc cacgattgcg gcggacgtcg gacagacgcc
      241 gcagggctgg aacgcaagca tattttcttt gatacagaat atttctcaga cggttattct
      301 gccgattgcg gggcttatta tagcctttgt cttatgctat gagctaataa ctatgattac
      361 ggaaaagaac aatatgcacg atatagacac gtttatgttc ttcaaatatt tcttaaaggc
      421 tggggtagca atttatattg ttacccacac ctttgacatt actatggcgg tatttgacgt
      481 tgcgcagcat attgtaacga acagcgcggg ggtaatcggt gcgaacacga acattgatat
      541 atcttccgcg cttgccgaca tacgaactac tcttgaagct atgggaatgg gcgaactgtt
      601 cctattagtt ttggaaacct cgcttgttag tttatgtatg aaaattctat ccgtttgtat
      661 taccgtcgta atattcggac gtatgataga gatttattta tattgctcgg ttggagctat
      721 cccgtttgcg acaatgacta atcgcgaatg ggggcaaatg ggaaataact atttgcgcgg
      781 acttgtggca ttaggctttc agggattttt cattatggta tgcgtaggca tttatgcggc
      841 gttggtaaac ggttttgttg taaccaataa tctgcatact accatattta cgattgcggc
      901 atatacggtt gtactatgct tttccctgtt caagacaggt aatctgtcaa agtcaatatt
      961 taacgcacat taaaaataaa aaaatgaaag ggggaatttt tattccttat gttcccgtac
     1021 caaaagattt aactaccgta aaaacaaagg tagcttttaa ccttacgaag cgtcagctta
     1081 tatgctttgg acttgcggcc gctgtcgctg ttccgacata ctttcttacg cgcggcgcgc
     1141 ttggcacaac cggcgcggtt atgacgcttg taatcgcggc cttgccgttc ttcttcctcg
     1201 ctatgtacga aaaggacgga cagccggcag aaaagatttt aagaaattat atacggtcaa
     1261 gatttttgac gccgagagtc agaccgtata aaacaaataa tatgtattcg gcaattatga
     1321 accaaattaa acttgataag gaggtcaaaa taattgccga agcagaatac aaacacaaga
     1381 ccaaaacaaa agaaaaagca cataatatca gcgaataagc cgctttcagc acgacagaaa
     1441 aaggctattg cggcagctat cagaaaagcg caaatgcagg gcaggcgcgc agcgtcagca
     1501 cagcagacta ttccatataa ggaaatgtac ccggacggga tatgtaaagt caaggataat
     1561 tattttacaa aaacaattca gtttttcgat ataaattatc agcttgcaag gaatgaggat
     1621 aaaaacgaga tatttgaaaa ctactgcgat tttcttaact atttcgacca gtcaatttct
     1681 gtacagtttt ctttcctcaa tcagactgtc gatatggcgg agtatgaaaa agccgtacat
     1741 attcccgaac aggcagacaa ttttaatgac atacgcattg agtatatggg tatgctcaaa
     1801 aatcagttgt cgcgcggaaa caacggtatt ataaagcgca aatttatcac attcggaatt
     1861 gaagcggata acttaaaggc ggctaagcag aagttagagc gtatcgaaac agatatactc
     1921 ggcaacttca aggctcttgg cgtacaggca agaccgctta acggattaga gcgtttagag
     1981 cttttacact gtactttcaa tgagggcgaa aaagacaaat tcggctttca ttgggatttg
     2041 gtacataaga cggggctttc ctcaaaggat tttattgcgc ctacctcgtt tgatttccgc
     2101 gacagccgca cgtttggaat gggcggcaga attggaactg tatcattttt acagatactc
     2161 gcgcctgaat taacagaccg tatgcttgcg gatttcctcg acctcgatat gggaataggc
     2221 gtaaatattc atattaagtc tatcgaccag tcggaagcta taaagacggt caagcggaaa
     2281 ataaccgacc ttgacaagat gaagattgag gaacagaaaa aggcggtcag aagcggttat
     2341 gatatggata ttataccgtc agaccttgcg acattcggcg gtgaagcaaa gaatttatta
     2401 gagcagttgc agagcagaaa tgaaagaatg ttccttgtta ctgttcttgt gatgaatacg
     2461 gcggataaca aacaaaaact tgataatatg tttttcgcgg cgcagggtat cgctcaaaaa
     2521 tataactgtg cattaaagcg tcttgacttc cagcaggaac aggcgctaat gtcaagcgtg
     2581 cctatcggat taaatcaaat agagatacaa cgcggattga ctacctcgtc aaccgctatt
     2641 tttgtaccct ttactacgca ggagcttttt caagccggcg atgaagcgtt atattacggt
     2701 ttaaacgcat tatcaaataa tatgatactt gttgaccgaa agaagctcaa aaatccgaat
     2761 ggcttaattt taggcacacc cggcagtggt aaatcattca gcgcgaaaag agagattgct
     2821 aacgctttcc ttgtaacgca ggacgatata attatatgcg atccggaagc ggagtacgcg
     2881 ccgcttgtgt caaggcttaa cggtcagatt atacggattt ccccgaactc gcacgattat
     2941 atcaatccaa tggacattaa cctcaattac agcgaggacg aaaatcccgt acaattaaaa
     3001 tcagatttta tactttcatt gtgtgagctt attgtcggcg gcaagaacgg acttgaannn
     3061 nnnnnnnccg attgaaaaaa gcgtgattga cagagcggtt ataaatatat accgtccgta
     3121 cctcgctaat cccgaaccgt ctaaaatgcc gattttggaa gatttatata acgagcttaa
     3181 agctatgccg gagccggaag cgcagcgtat cgcgtcggcg cttgatttgt atgtaaacgg
     3241 ttcattaaac gtgtttaatc accgtacaaa tgtaaatgta aataaccgta ttgtctgcta
     3301 tgacattaaa gagctcggca agcagcttaa aaagctgggt atgctgattg ttcaggatca
     3361 ggtatggaac agagtaacaa ttaaccgcgc ggagcataag gcgacgcggt attatgtaga
     3421 cgaatttcat ttactgttaa aggaggaaca gacggcggca tattcggttg agatttggaa
     3481 acgctttcgt aaannnnnnn nnnggcataa cgcagaatgt caaagacctg ttatcgtcaa
     3541 gagagattga gaatattttt gaaaattccg attttatcta tatgctcaat caggcaggcg
     3601 gcgacagaca gattttagcg aagcagctta atatatcccc tcaccaatta tcgtatgtaa
     3661 cgcagtcggg cgagggcgag ggacttttat tttacggaaa tgtaatcatt ccgtttgtag
     3721 accgtttccc gaaagacttg aagctgtatt cgtatatgac gacgaagccg gaggaaatac
     3781 agaaagatga atgatttaac gcatttaagc ctgtttacag gtataggcgg aatagacctc
     3841 gccgcagaag cagcggggtt taaaaccgtc tgccaatgtg aatgggcgga tttccagact
     3901 tccatacttg aaaagcggtg gcctcgcgtg ccccgatttc gtgatattac aactctaaca
     3961 aaggaggtat tttttgaaaa aacaggacga aacagcgtta ccctcattag cggcggattt
     4021 ccctgtcagc cgttttcaag cgcggggaaa caaaaagggt ttgaagatac ccgctacttg
     4081 tggcctgaaa tgtgccgcgt cattacagag ctcaagcccc gctgggtgct tggcgaaaat
     4141 gttgctgggt tcatcaatat ggggctcgac aaaacgattt ttgacttgga aaaagcgggc
     4201 tacactgttc aaacattcgt attaccagct tgcggtgtcg gcgcgtggca tcagcgcaca
     4261 agaactttta tcgtcggcgt tgatgtttcc cactccgctt gcctccgaca acaacaaaaa
     4321 gcagacggcg gctgtaaata tgaatgtgta tctttcagac aacgggatat ttcggaagaa
     4381 gaacaaaaac ggcgcaatct ggagccttcc gctgtcggcg gcggtgttct atatgacgcc
     4441 ggtagcgtcg gacgggataa ggtcaaaatt tccgccggag gtaatgagga aaagcaagga
     4501 cggggcgaac cttgcggcgc aggtgatatt agaggaacag ccgcagtcgg agacggcggc
     4561 gctcaatccc gattgggtgg aatggttaat ggggttcccg aagcaatgga cggatttaag
     4621 ttgtgggaac gggaacctgt cggcgttccg cgtataacgg acaggacgga cggcagagcc
     4681 gacaggctaa aagccttagg taatgccgta tgtcctccgc aggtgttccc tatacttaaa
     4741 ttcatttcag atattgaaac gggaagatgt aagaaatatt gtgagtttgg aggtgagcag
     4801 atataagcag agaaaacaag gattttctta acggcagcgg gtgcaaggat tttacggcgt
     4861 atgaagcaat caggaatata gagcgcgcgg aacgcaaaaa gctgattgcc gatattgccg
     4921 agcttgccgc aagacgcggc tatgagattg taagccctat ccgattaaag gaattagaat
     4981 aaaaaatgtc aggaggtata tatgtacaaa gattacgata attaccgtac ttatgaaaat
     5041 gacgatttat cagccggcgc cacacttaaa gttgagcata ccataatcgt taatgaaacc
     5101 gatatattga atttgatttc acaaccgctt gaaaaaatac aggctatgcg tgataataac
     5161 gtcaaaaaag aaacagccgc ctttgaaaaa gtacggcagt cagcgcggga ttgggaaaaa
     5221 aaagcggcaa ttacaagaca atttgacagg gctattgagt atttgcaagt accggaggtt
     5281 tcgcattctt caaataaatg ggtaaacgac agagattgtt ataactacga ttgtacatct
     5341 atcagtaata tggtatataa aatgacatac cgtatgtatg attacacaag ctggcgttca
     5401 gaaaaaacaa gacgctggga agtgaaatgg tctattggca caaatagtcc agttggtcat
     5461 aataactgta ttgcagaaca agaacgctca tttaaggata aggcggaagc ggaaatatat
     5521 acaaggcaga ataaaagcct attcacattt attcacggaa atttccccgc ccattccgaa
     5581 agaatatgaa aaatccttta tggtacacga acatcttcta cccggatatg ttacagagaa
     5641 aatgcaaaaa gctcaggaag cggcaaagac ggaaaatatc agccgaagcg cagataaaaa
     5701 gccgtcaatc cgcaaggagt taaattccat taaggcggca gagaaaagcg tgccgaagcc
     5761 gacgcagaca aaaaagcgcg ataatctcga actttaacag cggaggtaat gaatgaaaga
     5821 caatgaattt aaggcgcgga ataaggacac ggatttttca aaaaaggttg aagccgatac
     5881 cgttctcgga cggcagagcg caaaaggcag cgcgaagccc tttacttctt cagaacataa
     5941 taggtatcag cagaaatttt tagataccga aaagccgctt gatacttcta ccgtacaaaa
     6001 tccgtcagac agtcaggaaa tattaaagcc taccgaaaat aatacagaac agcaagagga
     6061 cttagagaat attaaccttg atagtcagcc ggatatacca tattccccgc cgacaaacga
     6121 gccgcccaaa agtggcggtt tttctaatgc cgtaactacg gcggcaagaa ataaacggta
     6181 tcagcgcaaa tttaatcaaa agaaatttga tgttgatatt actgaacatt ccgcagatag
     6241 tacggcggaa gaaacgcagc cggatagtgc cgaagcctta aaagataacg gaaattatga
     6301 ctttaccgag caaacacata atcctgatga agcgtttgac cgtgtgaacg gcaattcgga
     6361 ttttcagctc ggcgcggatt ccgaaaagga ttccgcagac ggcaatccga agcagccgga
     6421 taaaaaccga gcctatcaga aaaagcgcgt caagaccgaa aaaacagcag agtacaacat
     6481 caaagaagcg gttgacgcag gcgatacggt ttttgacaga gtaacggaaa aagaggattt
     6541 tgtttttgaa acgcgggaaa gcaagacgga attttcattc cgtccgtcag aggataaaac
     6601 cgaaaaacag acagccggaa gcaaaaaccg cttatatcag gaaaagcaat ccaaaacaga
     6661 gtcggcggaa aacgccgata ctgttttaga tattcccgaa aagggcgatt ttcagtttaa
     6721 gcgagcggaa aaggcgcagg cgaaagccga taagctcaaa ggcaaggcgg aaaaagcaca
     6781 aaacaaatta cctcataagc gcaaggttaa aaagcagcgt gtttatgatg aaaagaaaaa
     6841 gaaagctaaa acccgtctga aatttgaaaa agagattaag cctcaatccg atatatacca
     6901 ccgttcccct gtaaaaaatg cggcggatat ggcaggtatg accgtattaa acaaggcaca
     6961 ttcaaaagtg agcgaaacag aacacgaaaa tacaggaatt gaagccgcgc ataagacaga
     7021 acagaaagcc gaaagcattt tacggtttgg aaaccgtaca gggcgtatga ttatacaaaa
     7081 ccgcaaaaac gcgccgtata aaaaagcggc gcgtctgcaa ttcagagcag agaaagcgga
     7141 agcaaaggcc tttgttcaaa aaacacttgc ggataatccc gatttcaaga aaaaatcagc
     7201 tttgaggaaa ttttttcaga aaaagcagat taagaaaaaa taccagcagg cgaagcgcgc
     7261 cgaacagaac gctaaaaagg caaaacgagc cgcaaagacc acaaaggacg ttacggtgta
     7321 cgttgttgaa tttataaagc gtcataaaaa ggtattcggc gtcattcttg ctattgcttt
     7381 aataattgct tggatagcaa ctacaatttc atcttgcgcg gttatgggta caacgggctt
     7441 taactcaatt atggtatcgt catactttgc ggaggacgaa gatatatacg cggcggaaaa
     7501 ctattacaaa gaccttgaaa gcggtttaca aagtgagata aacaatattg aacgcgatta
     7561 cggaggttat gacgaataca attacgattt ggcgcagata ggccataatc cgtatgagct
     7621 catttcctac ctcacggcgt tgtatatgga tttcactttt gatgatgtaa aacagccgct
     7681 tgatgatttg tttgaacagc agtatatatt aacgcttaca tctcagacgg aaacaaggac
     7741 ggacgacgag ggcaacgaca aggaatataa aatactcaat gttacgcttg taaacaagaa
     7801 tattactatg cttacaaata cgaaccttac ggacgaccag cgggaattat acggtatata
     7861 tcttgaaagc aagggcaacc gcgattattt atttgcggac gatatttatt ctaatccggc
     7921 agcgccgccg agttatacca tacccggcga agcattaaac gacgctacat tccgacgtct
     7981 tataacggaa gccgaaaaat acttaggtta tccgtatgta tggggcggtt cttcaccgtc
     8041 aacgagtttt gattgcagcg gttttgtatg ctgggtatat caggcgtcag gcgtatatcc
     8101 tctatcccgt acaacagcgc agggtatttt taatcaatgc gcggttgtgt caagagcgga
     8161 cgcaaaaccc ggcgacatta tattttttac aggcacatat aacgcgggcg agcctgtaac
     8221 ccacgtcgga atatttgtcg gtaacggtat gatgatacat tgcggcaacc ctattcagta
     8281 cgcgagtatt gatagttcct attggagttc gcatttttac gcctttggca gacttggggg
     8341 cggttgatat gcgttatctt tacagttgga gcggtggcaa ggacagtacg gcggcaataa
     8401 tacttagtca tattcataac ttgccgccgt caaccgtcat tttcagcgag gtaatgtttg
     8461 ataagaaacg cggtatttca ggcgaattac ccgaacatat agactttatc aaaaacagag
     8521 ctattcccgt ttttgaagaa tggggttata cggttgaaat attacacgcg acaaaagatt
     8581 atttagattg ttttcatcag ataatcacaa aatctaaaaa tccgttaaga aatggaagaa
     8641 aaagcggttt tccgataggt ggaatgtgta caattaacag ccgtataaaa ataaaagcaa
     8701 ttcaggatta tttgaaaaat gtaaatggcg gatttgtaca atatgtcgga atagccgcag
     8761 acgaaccggc gaggcttaca aggcttgacg gtacaaataa aatatcatta cttgccgaat
     8821 atggctatac tgaacaaatg gcttatgatt tatgcagcaa gtatgatttg ctgtccccaa
     8881 tatataagta tcacaaaagg ggcggttgtt ggttctgtcc taacgcgact tacggcgagt
     8941 ttgcatattt gaagcaatac caccctgacc tatggaatga gcttgaaata ttatcaaagg
     9001 acaaaaacct tgcaagtcaa aagttcaaat atgggaatac attttccgaa gtcgataaaa
     9061 agattgatga atttatcgca aaacaaaaaa tttaatttta tggaggtatt ttaattatgg
     9121 caacacttga aaaaatcgaa aaggatatta taaggacaaa agcaaagata agcgagtatc
     9181 agcaaaaatt acgcaatctt gaagcgcaga aagtcgaagc cgagaatttg cagattgtaa
     9241 atctcgttaa ggctgttaaa cttagcactc cccagcttac cgtactttta agcgcgtatg
     9301 caaagggtga tgttcttctt cccgacgagt acgaggagga attaaaggct attgaggaaa
     9361 acgagcagca ggaggacggc acgaatgaag aataaaatta ttgcgatatt atcagcagct
     9421 ttattatgct ttggcggaat gtgcggaata accgcacacg caaactatga cagtacagca
     9481 gcagcgccgg cggcaacgga aacgccgaag ccggcgcgta ccatatcaac agcgggcgcg
     9541 gggcacgttg tagacaacat ttccgacagt gataaattgc agtttataag cgtaaccgca
     9601 aaggacggca gcgtattttt tatcgttatc gacaagcaga atgtaaacga taatgtgtat
     9661 ttcttgaata aggttgatat atccgacctt gaagcgcttg caaaggaaaa cagcgcggca
     9721 gtacccgcag cagtaaaagc aagccctaca ccgcaggcgc aggcgtcgcc gtcggcggag
     9781 ccggacggta agacgaaaac cgcgccgaaa accgataaat caaagtcagg caattcaagt
     9841 ataatgctta ttctgattgc gattataggt ataggcggtc ttggcgcgta ctatttcaag
     9901 gtttatcttc caaaaaagaa tatggaaaac gcccccgatt tggaagattt tgaatttacc
     9961 gacggcgagg acgaggaaac cgaaaacgag gacgatacat atacagaaga tgaataaaat
    10021 atattgcaat attactttgt ttatgatata atatttattc aagaaatttt atacttacta
    10081 acaagtagaa aggagattgt atatgagtaa ggttgatgaa tatacaggta atggaatgat
    10141 tgtagttagt gatggtgaag tttgggcggt agatgatagc ggactacctg atgtaatagg
    10201 agaaatagga cgtgttgaat tatccataga aatgccagaa aacttaattg gcatatatcg
    10261 agttgagcat ataatgttat ttgatgaaga tgatgaggaa ttatatgacg accaaacatt
    10321 agtggataat acggaatacc attcagaaag agcgttagta aaagcagttg caaaaaaata
    10381 cggtatttca gaggatatta taactgtttt ataaaggata atatacaacc acaaaaacag
    10441 cactcattgt tgggtgctgt ttttgtatgt aaaaataaag gaggaatgat taaataaatg
    10501 aaattagtaa ttgcggaaaa nnnnnnnnnn gaacataacc gctaagaaaa gcggatattt
    10561 tgagggaaac ggatatatca ttacttggtg tatcggacat cttgcaggct tggcggacgc
    10621 agcggcgtat gatgaaaaat acgcggtatg gaaatatgag gatttaccta tcctacctga
    10681 aaaatggcag tttgccataa acaagggcaa ggaaaatcag tataaggttg tcagcggttt
    10741 aatgaaccga aacgacataa ccgagattat aaacgggtgc gacgcaggac gcgagggcga
    10801 attgattttc agatttgtat ataatatgtg tggctgcaaa aagccgtact tccgtttatg
    10861 gatttcctca atggaagaaa gcgcaatccg cgagggattt aataacctca aagacggacg
    10921 cgagtatgat aatctttata attccgcgtt atgccgcgca aaagctgatt ggattatcgg
    10981 tataaacgca acaagactgt ttacatcact gtatagaaag catttgaata taggacgcgt
    11041 tcagacgcct acccttgcta tgatagcaga acgggacagc aaaatttcaa tgttcaggaa
    11101 agaaaaatat tataccgttg aaattgacgg cggcaatatt aaggctgtaa gcgataagat
    11161 ttccgacaaa gacgaagcgc agaatttaca agcggcttgc cacgaaaggc aggccgtttg
    11221 tatttccgct gttaaagaga aaaagaaaac aggcgcgccg aaactgtttg acctcacaac
    11281 attacagcgc gaagcaaacc gaatattcgg atatacggcg aaacagacgc ttgactatgc
    11341 tcaatcgctt tatgaaaaga agctgattac atatccgaga accgacagcc gatatttaac
    11401 ggacgatatg cacaatactg ttattaaaat cgttcatata gcgtcaaaat tcgcgccgtt
    11461 ctcaaactgt acggatttta agcctgatac attctgctta ttcaataatg aaaaggtatc
    11521 agaccaccac gctattattc cgacggcaga aattgaaaat atcagcttgg cggaaatacc
    11581 aaacggcgaa agtaatatac ttcaccttat tatgtgcaag ctgttatgtg cggttaattc
    11641 cccgtatgag tatgaaacga ttaccgcagt tttggagtgc ggcggcgtac agtttacggc
    11701 aaagggtaaa atcattttat ttgagggctg gaaaaatatc gaacagcttt ttaaaagata
    11761 tattcattcg gacacagacc gcgccgaaga tgtattttgt attacaaaag gcgaaaccgt
    11821 taccgtttca tcagaagtca aagagggtta tacctcaccg ccgaaagcgt ttacggagga
    11881 cactctatta tcatcaatgg aaacggcagg agccgccgaa acaacggacg aagcggaacg
    11941 caagggcttg ggaacgccgg cgacaagagc ggcaattata gaaaagctgg tacagacagg
    12001 ttttgcagag cgtaagggca agtctattgt tgcaactcat aacggtataa gtcttgtcag
    12061 cgttcttccc gaaaagctca catcacctat tctaacgtcc gattgggaaa ataaccttgc
    12121 cgagattgca aagggcaatt atccgccgga gcagtttatg aaaggaattg aggatttgac
    12181 aagggagctt gtaaccacat attcattcat cacagacgag gacagagcga tattccgcgc
    12241 gccgcgtgag gttatcggta tatgtccccg ctgcggttcg gacgtacacg aaagcaaaaa
    12301 taacttttat tgcgctaata aaagctgtaa attcgttatg tggaaaaatg acaggttttt
    12361 cacaagcaag cgaaaagaga ttacaaagaa aatggcgtct gatttgctcg caaaaggcag
    12421 aacaaaggta aaaggcttgt attccgagaa aaaggataag acctatgacg cggacgtggt
    12481 tttaatcgac agcaacgata agtatgtaca tttcaggctg gattttgacg gaaagtgagg
    12541 ttgatatatg gcagatacag aaggcacacc agattttcac ttggccgctt atgttaccaa
    12601 tcttggaaaa tataacgagg gatatttagt aggcgaatgg gtgaagttnn nnnnnnnnta
    12661 ctcctgatga aatgcaagat gtatttaggc gtataggtat aggcggaaaa gatgtattcg
    12721 gttcgccgta tgaggaatgg tttattacag actatgattg ttatattgac gggatatatg
    12781 atatgttgag cgaatatgag agccttgacg aattaaatta tctcgcagac aaattagatg
    12841 aaatgtcgga cgcggaagta cagcatttta aggctatcgt taaaatgggc gaacattcag
    12901 acagtgtaca ggatttgata aatctaactg aaaatcttga ttgctacgaa atatatccag
    12961 atattacaga cgagtatgat ttgggatatt accttatcaa tgaattgggc tgttatgata
    13021 tgaacacact gaaagcattg gaaaattaca ttgattataa ggagtatgga gatgatactg
    13081 cgctgaatga aaacggtgta tttacggatt acggttatgt tgtgtgtaat caaagtgatt
    13141 ttaaagagat atacaacggc agccgtgatg atattcctga cgaataccgt ataagctcta
    13201 tacnnnnnnn nnntgaagct gaacagaaag acgctgtaaa aacagaagaa aaaaagccgt
    13261 caattcgtca gcagcttgcg gaaaataagt caaaatccga aaagacgcaa tccgtaccgt
    13321 taaggcaaaa aaacgatttg agcctatgag gtttggtatc tgatgaaacg tattgacata
    13381 acaaacggaa ttatgcggat tgatactaat acggaaacat ttgatattca gaaaatccaa
    13441 aaccgccgtt ttatgtataa tccgcagaca ggcacgttga ttttgggaat acaaacacaa
    13501 tgcaacgatt ttttttataa aaaggttacg gaatatcggc gtataggtat aaggtcaaat
    13561 ttcaa
//
LOCUS       DAWACS010000002        12910 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_585_length_12910_cov_2.577518, whole genome shotgun sequence.
ACCESSION   DAWACS010000002 DAWACS010000000
VERSION     DAWACS010000002.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 12910)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12910)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12910
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_585_length_12910_cov_2.577518"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6007..6016
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7622..7631
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9906..9915
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3937 a         2285 c         2833 g         3825 t
ORIGIN      
        1 ttccattgac agatagaacg gattttgtat tttgtattga tttatagcgc tgtttagttt
       61 tgcaagaaga ttggtattgc ctttttttac ggcacagtag tagtccaatt tgccgccttt
      121 ggctattaac tttaatccgt cgggattgtt tgttgtaggc gtaaaaattg cgtcgattga
      181 attgtttttc acggcattta acatatcgtc cacagtatcg taataaacag ggatgtatgt
      241 aaaattattt gcagccgaaa aatcggaaag aattttttct atatacgaat ttttaagtaa
      301 accgatttta atctgattga atgtatcaaa ttcttcgaaa tataattgct tgtaatcggt
      361 ggtataaagt cctaaaaagc ctgttgcgat aggtgatgat gtatagtcaa catacgcaaa
      421 aaaggcatca ctcacaggaa ccatacataa aaaatctata tttccgcttt gaaggtcgga
      481 aataccttta ttaaaagttg tgtcatcgaa gtttatttcg ttagctataa aatttgtact
      541 tgcagttgta tctaaaatag tatttacact atcttcggca tattcattga tactgttgcc
      601 gtcaaagctg taatcatcaa gaataccggc ttttaacgta ttcgtttcag catataccga
      661 aaaataagta aagcaaataa cagatgttaa aagcattaaa ataattgctt ttatgttttt
      721 cattcaaatt cccctttgtt taaatcctct ctctcatata ttatatacca cattgtccaa
      781 atgttcaaac aaaaaattaa aaaaatataa aaaaatattg cagacaaaac ctttaagggg
      841 tatctgcttt atatttggta tacatattat aagttatcgt gcatatacat ataacatata
      901 ttttggacaa aaggaggaaa caaaaagtgg aagaatacaa tatttacagt gatatagcac
      961 agaggagtca aggagatgtt tatataggtg tagtcggacc ggtcagaaca ggtaaatcta
     1021 catttataaa aaagtttatg gatttgcttg taatacctaa tatagaaaat gtgtatcagg
     1081 cagagagaac acgagatgaa ttgccacagt ccgccggcgg cagaacgatt atgacgacag
     1141 agccgaagtt tataccgaat gaggcggttg aaatatccct gggcgataat gcacatttaa
     1201 aagtgagaat gattgactgc gtcggctata tcgtggacag ttcgcttgga tatgtggagg
     1261 ataacgagcc gagaatggtt acaactccgt ggtttgacca cccgattgct tttaatgagg
     1321 cggcggaaat aggtacgaaa aaagttattt gcgaacattc gactatcggt cttgttgtta
     1381 cgactgacgg tacgattacg gagattgaca gaaacgatta tgttgacgcc gaaaacagag
     1441 ttataaatga attaaaggcg attaataagc cgtttatcgt actgttaaat tcggtagcgc
     1501 ctaattcaca gagtgcacag aatttaaagg ctgaacttga ggcaaagcac ggtgtacctg
     1561 ttgtggcggt gaattgtgaa gaacttaatg caaaggatat acataatatt attgaaacgg
     1621 tgttgtttga atttccgctt aaagaaatta atattaaaat tccagattgg attgaagaac
     1681 ttgacagtga gcattggctg aaaaaagaga tatacggtgc gataatcgaa aaaatcgagg
     1741 acgtaaaccg tattcgcgat gtgcgtgcat tgtctgacgg tatgggtgag tgcggttttg
     1801 ttcagcgttc gtatatcgag ggtatggatt tgggtgacgg tacggttaag ctgtgtatgg
     1861 aacttccgca ggagcttttc tatcgtgtgc ttggtgaaat gtccggcttt gaaattgacg
     1921 gcgaacatca gcttatgacg cttatgtcgg agctggcaca gatgaaagca cagtatgata
     1981 aggtgtcatt tgcacttcac gaggtgatgg aaaagggtta cggaattgtg tcaccgtcaa
     2041 cagaagaatt gactcttgac gaaccgaaaa tcgttaaaca aggcggtaga ttcggtgtta
     2101 aacttaaagc gtccgcaccg tctatacata tgattcgtgc ggatattgaa acggaagtaa
     2161 gtccgattgt cggtactgaa aaacagtcgg aggagcttgt gcattatctg ctgtcggaat
     2221 ttgaaacgga tccgacaaaa atttggcagt cgaatatatt cggtaagtcg ctttatgagc
     2281 ttgtaaatga gggacttcat aataaacttt caagaatgcc tgaatcggca agaatgaaat
     2341 taaaagaaac tttgcagagg attattaatg acggaagtaa cggcttgatt tgtataattc
     2401 tgtaataagg ggcttgataa tgaaacgtat aatagctgta atcttcgtga ttctttgttt
     2461 gtgcgggtgc gatatgaaac gtatccgcac agaaagcagt ataaaggaat atgaaaccga
     2521 tttttcaacg gtgtatgccg aaacagttaa attcagcggt atgaaaaaca gtgagtttga
     2581 aaaaaatatc aacgcacaga tacaacagtc catagacagt gaccttgttg cttttgattc
     2641 caaagcacag gaatgtaagg ataatttgca aatggggaac aagtgcgtta tggagatagg
     2701 ttgggaggaa acttataata aaaatgattt tatcagtgtg gttgaagaaa aatatattta
     2761 taccggcggt gcaagaggta caacggtgca ttttcccgta aatatagatg tgtatggtga
     2821 aaaggaagtg aaacttgcgg atttgtttgt tgatgacggg tatgtcagta cgcttaacag
     2881 aatgattaat gaagaaatgg aaaaacattc ggaagaatat aaggatttgt gggcaaagcc
     2941 tgaaataaaa caggaacatc agacggattt ttatattcaa gacgatgatt tggttatatt
     3001 ctttcaaccg tatgatttgt catactatgc ccgtgggttc gtggaattta gacttgattt
     3061 ggaagatttg tccgggtata tgaaagagga atatagacgg atgattgatt aggggcgacc
     3121 atcggtcgcc ttttttgcgt ttgtgtgagt aggggacggc gcccacgacg tcccttttcc
     3181 cacatcaatt ccgtaggggc gatcatcggt cgccccttgt tcaacacact taaacatcga
     3241 tttcaaaatg ataactaccg cctacaagtg tatgaatatc accgttaggc aatttcaaag
     3301 ttgcggttgt attagtcgga actgtgcaat catacgtaaa tttaccgtca tgcaatttcc
     3361 acgcagaact gattttaccg taaggtgacg taaaactgcc tttggcataa gtcaaaccgc
     3421 ctccgaattt cggtgcaagc ataaatgatt tatacccaaa ttcaacggga cgaataccga
     3481 gtatatcggt aaagaaccat tgacctatcg caccgtaaga ataatggtta aacgaattca
     3541 ttgaaacatc gccgaaagtg cctgtttcgg caatatacga attccaacgc tcccatatgg
     3601 ttgtagcacc gtttttgaca ctgtaaagcc aagacggatt gtcttcctgt tcaagcaact
     3661 taaaagcaac atcatcaaga ccgacttttg aaagtgcagg tgcaagatgt gatataccga
     3721 gaaagccgac tgtaagtttg ccgttgtttc tcaatattga gtttttgaaa tattctcttg
     3781 tatttttaag cttgtctttg gggataaggt caaagtcaac tgcaagtgca tatgctgttt
     3841 gactcggaca gtgcggagtg ttttcggcat aatatgtata tcgcgtaaca cttccgtcaa
     3901 gtgcgaccga ttcgccgcaa tgtgcggtct gtgaactcac ttcgccgtaa tacgaaacag
     3961 agccgtcatc attaacccac gcatcattga atgcacgttt tatattttca aataattcag
     4021 tatatcgttt ttcatcatcg gttttgccga gaatgtacgc ggttctgcgg agtatgtcgg
     4081 cagaatgtgc ggcatatgcc gttgcggaat atatgtaagg cgtggaattg tcataagtaa
     4141 ggtggtcgcc acggcattgc tgtacttgaa tatacggaat tgtcgcaagg tcggattgtt
     4201 ccggtacttc cttgccgtca accgttcgta caccgtaatt acggcggtca gtgctgtttt
     4261 gatattcaac ccactttttc atcatatcat agcatttttt aagtatgcgt atatcgccgt
     4321 atgcttcata catttcccac ggcacaatcg ttgcggcgtc gccccaaccg ccgcaaccgt
     4381 caatgcggtt atcacctccg agtggggcgg tatcgggaac agcaccgttt ttattgtaca
     4441 tagtttgtgc gtcaatcaaa tcatcaagcc atttgtcgat aaacgacttt atgttcatat
     4501 tatatgcacc tgtttttgca aaaacctgca tatcacccgt ccaacccata cgctcgttgc
     4561 gttgcggaca atcagtagga attagcagaa aattgccgat ttcaccccaa cggatattgt
     4621 gctgaagcct gttaataagc ggatttgaac attcaaattc acctgtaaca atatcaatat
     4681 tcgatattgc aagaccgtca accgagataa tcatatcttt tgatatatcg caaccgttac
     4741 cgctgatttc cgcatatctg aaaccgtgtg acgtgaaagt aggaataaac tcctccgtat
     4801 cacgtccgca aagtgtataa atatcggtat ttgcggcgga acgcagattt tttatataaa
     4861 tacagccgtc tttatgcgtc atttcgccgt aacgaatttt aatcgactga ccgcattgac
     4921 ctttaacttt caattttatt gttccgacca tattttgacc gacatcataa acgaaatgat
     4981 ttttcggatt ttcacacacg aatttaccga caaagttttt tataattttt gcagtgttac
     5041 ccttttgggc ggttaattta aaatcagtat cggatagtgt gccgtttgtc gggacaggct
     5101 ttttaggttg ttgtttgata ccgcatttaa cccagcgttt atcgtccgca aggggattgt
     5161 attcaagcct tgcgtcatag ccttcgccgt caaaataatc acagtcgctt atcggtgctt
     5221 tatcagtaaa ctcccacgaa ctgtctgttt tgataacctc gcttgttccg tcagtgtaga
     5281 caatattcag catagcaata aacgaactct ctttgccgta tatcatagca ccgctgtaac
     5341 cgcaaaagcc cgaataatgt cctcttccaa gaactgcact tatgatattt ttaccgtctg
     5401 caagcatatc ggtaacgtca aaagtttggt attgaattct tagtctgtag tcggtaaaac
     5461 cgggattgta aaaatcgtta ctaacttttt cgccgtttat gcaggcattg taaaaacctt
     5521 gcgcagacgc ataaagacgt gccgatttga ttgttttttc tatattgaaa cgtttgcgga
     5581 gatttacaga cggtacggga ttgacaattt caaatgcgtt gttgattgtg agttttccgt
     5641 tttcgtatgt gccgagtgcc gaaagtaagc cgttttcaaa attatcctct tgcaaaacaa
     5701 gattgtttgt ttcgattttt aagtgactga tttcggcaac actgtttagc tgattaaagc
     5761 cgacagacat taaaaatgtc ttgcgtggct gatttatgat atttttcgga agaatatctt
     5821 catcaattac tttttgaccg ttcagataaa gaagtacatt tgttttgttt accgataatt
     5881 caaggctgtc ggaatgttcg gtaacatgga atgtaccgag taatttttta tacggaatat
     5941 tgtcattcca agcattgtcg gatatttcgt aaacagaaac gctgtctttg cagatgttaa
     6001 ataggannnn nnnnnngtgt tgacggtttc ttgcggactg ccaatccaat cggaattaaa
     6061 ttcattattc attaagcctg tttcaaaatc ggcagtatcg cttgtgtatt cattgccttg
     6121 ttcgtcccat acgcttactt gccaagaata ctttgtcata gccgataaag actgaccgtc
     6181 gtattcaatg cagattgaac ggtcagatgt aactttaccg ctgtcccaaa ccgtatcatt
     6241 gtcggagcga acaataattc tgtaagcggt ttgaagagta ccgctgattt tcgattgcag
     6301 tttccaagaa aatcgcggtt tcttttcatc aattccgatt ggatttatta aatcctctgt
     6361 tttcatatcg taaataatca taattctaat ctccgtctgt tttattaata tcattatacc
     6421 atagcacaat ataaaatgtt tcaaaatttt gcttgaaatc gtttattttt tgctttgtat
     6481 tttgacattg catataatat ttgatataat atgcaagtta aaagaaggga gagattagaa
     6541 tgaagaaaaa tgtaaagaaa ataacggtcg gaggactgct tatcgcattg gcacttgtat
     6601 tgcctatggc atttcattta acgggagtgc cgcaaccggg tcaagtattt ttgcctatgc
     6661 acatacctgt attactcgga ggatttgttt taggacctgt gtttggattt tttgtaggat
     6721 tgttttcacc gattataagc tctgttttga cgggtatgcc ggcagtaggc agattgccgt
     6781 ttatgatgat tgagcttgcg gtatatggat tggtaagcgg tttgatgtat aatacattta
     6841 agtttaataa gaagaaaatg ggtacatata tttcactttt aactgcaatg ctttgcggta
     6901 gaattgtgta tgcaatttca ttgtttgttg cggtaaatct tatgggtata cagtgtggcg
     6961 gacctattgc ggcagttacc gcaacagtca gcggtgtacc tggaattata attcaagttt
     7021 tgataattcc gcctgttgta tatgcacttg aaaggagcgg ttacattgac agacatttta
     7081 gaatctgcaa aggaacagtt gcataataat aatttttcta ttgtgattgt aggcgaaaat
     7141 gatgtttata cttcaacgaa acacggcgtt gcacctcttt tggaaattct tgacggtggg
     7201 gatgatatta taaaaaattc gtttgctgcc gataaagttg tcggtaaagc ggcggcttta
     7261 cttatggtaa aaggcggagt aaaagaggtt tatgccgata ttataagctc tcatgcactt
     7321 gatgtttttg aaaaatataa gattaagaca tattacggta atttggttga gtatattata
     7381 aatcgtgata aaacaggtat gtgtcctatg gaaaaggcag tgcttgaagt agatgaaccg
     7441 aatacggcat atgatgtgct gaaaaagaaa attgctgaaa tgagaaaggg cggtcattga
     7501 tcgccttttg ctatataata aatatttcgt agggcggccg ttggtcgcct ttttgtgtgg
     7561 ttttgtattg caatacggca agtgtttcgt aggggcgacc gatgattgcc ctacggtttt
     7621 gnnnnnnnnn ntcctttttt gccaccactc cgtcactgcg tgccacctct cctcaaggag
     7681 aggcattttg taaggctccc attgagggga gctgtcggca aaaccgactg aggggtggtg
     7741 atagaaaaat aggcaacggg cgaccgatga tcgcccctac gaaatataaa aacgcacaaa
     7801 aaaacaccgc cgtatcggcg gtgtcaaaaa catatacata ttggggggag taaatgtttt
     7861 aaatggggga gttatcacaa ctataaaaga tgtaatatat atatgaaaca aatatcaaag
     7921 atatttgtaa cttttggggg gacccttgcc tttggggaag gcaagggcat atgaaaaaaa
     7981 ggatggtatc gactgttttt acacagtcta tcaattaaaa ctggatagct tatttagttt
     8041 tatctgccga gtcttccatc aagcgaacct gtacttccat agtttcaccg tctcttaaaa
     8101 tcttaaaggt caaataatcg cccgcttttt tcttatcacg gatttcgttg acctcctcgg
     8161 tagatgagac cttctgaccg tcaacttcaa gaatcatatc gttgacttta agtccggcct
     8221 ctgccgctcc gctgccctcg gaaacacttc cgataaacaa accgtagcct gtatctttga
     8281 taccgattcc gacaagcggt ctgcctgtca cgtatcctga tgccatcaag tcgtcaatga
     8341 ttggttttgc ctctgatatt gcgatagcaa atcccatacc ttcaacgcct gtggttgata
     8401 atttaactga gttaatacct ataacctcac cgtattggtt gattaaagca ccgcccgagt
     8461 tacctgagtt aatggcggca tctgtctgca ataggttata agttctgtta tctatattca
     8521 ttgttctgtt tacggcactg attatacctg ccgtaactga accggaaaat tcctgtccca
     8581 tcgggttacc gattgcaact gccgtttcac cgacttaaac tgttgtcgag tcgccgcgtg
     8641 ctgcggctgt aagctttgtt gaacccggat ctattttcaa aaccgcaagg tctgtttttg
     8701 tatcctgacc tacaagtttt gcggtgaatt catcgcctgt attaagtatt accgatattt
     8761 ccgaagcacc gtcaataacg tgctgatttg ttacaatgta accatcgtct tggaaaataa
     8821 ttcctgaacc gctgccttgt tcaacggttt gaccgtctgt gcttccgtcg ccgcttgaat
     8881 aataatatct tccgctgaac ggatcgtaat atttttgcgg ttgaactgta gtcttgttta
     8941 taacaccgac aacgcttggt ccgacttgtt ctgcaatttc gggaattgta agaactttct
     9001 taccgttttt gtatgctgca agttgagctg aatccgaaga tgatgaatca tccgattgtg
     9061 ttgtttcact tgcaactgcc gcgctttgag tgttattgtt catattcttt tgcataaagt
     9121 acattcctgt tccgaatacg cctgctgtaa atatgcttgc tgcaagagct gaacagacag
     9181 ctgttagcca tactttttgt tttttctctt tctttggctt ttggtcgtaa ataatcaagt
     9241 cagttgattc tgtataaggg tatttcttca tggtcgtcat cctccttata tcattattat
     9301 caaaacaatc taaattcata ctctttatag ctttcctttg actatggtta tatgatacag
     9361 tacacttttg acactggtat gaattaaatg taaatagaaa tgacttattt tatgaacaaa
     9421 gtgtgaacag gtattgacaa atagagataa taaggttaga atatgtataa atgcgaatta
     9481 tttgcaacaa aatatacacg caaagatatt atatacacgc aaagatatta taaagtagaa
     9541 aggattttat atgaagaagt tacttatact tgattcaaac agtattttaa accgtgcgtt
     9601 ttacggtgtg cgttatttgt ccgcaaagga cggtactccg acaaatgcga tatatggttt
     9661 tcttaatata ttattgaaac tgataaagga gcaggagccg gattatattt gtgcggcttt
     9721 tgacgtgaaa gcaccgacat ttcgtcataa gcagtacgag ggatacaagg cacagcgtaa
     9781 gccaatgccc gagggacttg ccgcacaaat gcctcttgca aaggatgtac ttcgtgcaat
     9841 gggcgtaaca attcttgaaa aagagggtta cgaggctgat gacataatcg gaacagttgc
     9901 aagacnnnnn nnnnntgaaa taagctgttt cattgcaacg ggcgataaag acgatctgca
     9961 gcttgcgagt gacaaaacaa aggtcatttt gacggttaca aagtcgggat ataatgaaac
    10021 gattatttat gatgacaagg ctgttaagga aaaatatcac gtcacaccga ctgaatttat
    10081 tgacgtaaag gcgcttatgg gcgatccgtc cgataacatt cccggtgtta agggggtcgg
    10141 cgaaaaaacg gctatgagcc ttatcgaaaa acatcacagc attgaatata tatatgaaaa
    10201 tattgacgac ataggtctaa agggtgctat gctccaaaag atgaaagacg gcagggaaat
    10261 ggcgtttatg tcaaaggagc ttgcgactat caacagaaat acaccgattg aatttaatgc
    10321 ggaggaatgt gtatttgacg gttttgaaaa caacggcgag ctttatgaaa tattaaagcg
    10381 acttgaactt aacagtatta taaagaagct tgatttgtca ggcggggata atgtaaagga
    10441 aaacgaggat atttttaaag atttttctta tcaagtcggg gataagaata tgattagcgg
    10501 tgataaagtg accgttgtgc ttgactttga cggtgataat atttcatcgg cggcagtcgg
    10561 agcggggaat aatgcggtag tattaaatga gcaagacgat ataaaagagt tgcttgaaga
    10621 tgacagcata gcaaaggtta tgtttgacgt caaagaggct atcgttaaat taaacggcag
    10681 aattgacata aaaaatatat cagatgatac cgcaatcgcc gcataccttg tagatcctgc
    10741 aaaaaatgaa tacacaatag aaaaattagc aagcgaatat ttcggcacgg ttatagaaaa
    10801 gcctgaagtc aagcagttgt cattacttga cgatgtggaa acagacagga gcgaatacct
    10861 tgcaaaatgt gcagttgcac tcggtgtgct taatgagcgt ataggcgata agataaagga
    10921 aaacggacag gaaaagctgt atcaagaggt ggaattaccg cttgttacgg tgcttgcaca
    10981 ccttgaaata aacggatttc tcgtagacga caatcaatta aaggaatttg ccgataaact
    11041 cggtgaaaaa attgatgcgc ttacaaatga aatatatatg cttgcaggtg aagaatttaa
    11101 tattaattca ccgaaacagc ttggtgtaat tctttttgaa aaacttgaat taaagcctgt
    11161 aaagaaaaca aagaccggat atgcgacaaa tgcagatgtg cttgaaaagc tcagagataa
    11221 gcaccctata gttaatttta ttatggaata ccgtcagctt gcaaagctga aaagcacata
    11281 ttgtgacggt ttgacggcgg ttgtaaatcc gaatacgcac cgcatacatt cggtgtttac
    11341 gcagacagtg accgttacgg gcagactttc atcaacagaa ccgaatttgc agaacattcc
    11401 gactcgtacg gagcttggca gagagattag aaaaatgttt gttgcgaaag atggttatgt
    11461 gcttgtggac gcagactatt cacagattga acttagagtg cttgcgcata tcgcaaatga
    11521 tgaaacaatg ataaatgcgt ttagaaataa tgaggatatt cacgcagtta ccgcgtcaca
    11581 ggtgcttggt ataccgcttg aagatgttac aaaggaacag cgttcaagtg caaaggcggt
    11641 aaacttcggt attgtgtacg gcataggtga attttcactg gcacaggatt tgcatatatc
    11701 ggttaaagag gctaaggcat atattgaaag ctatcttgaa aagtatcacg gcgtaagaaa
    11761 ttatatggag agcataaagg aacaggcgaa aaaagacggc tatgttaaaa ctatgcttaa
    11821 cagaatacgt tatatccctg aattgaagtc accgaattat aatatacgtc agttcggcga
    11881 acgtgttgca cttaatacac ctatacaagg tacggcggcg gatattataa agcttgcaat
    11941 ggtgagagtt gataatagac tgataaatga gggattgaag tcaaagctta tattgcaggt
    12001 acacgacgaa cttatagttg aggcacataa ggacgaagtg gataaggtta aacaaatttt
    12061 gagcgaagaa atgcagggcg caatggaact taatgtgcct cttaaagtgg atatgtcaac
    12121 aggacatagc tggtatgacg cgaaatagtt tgccaccact ccgtcacttc gtgacacctc
    12181 tcctcaagga gaggcataaa aggcgcccct tgaggggagc tgtcgtgaaa cgactgaggg
    12241 gtggcaagta tagataatag ataaattcgt aggggacgac acccacgacg tcccgtatgt
    12301 gcgattatta catcaaaata acaatgaaag gaaataaaaa tatgaaaaaa atcataaagc
    12361 tgataccact aatgcttata cttgttttgg cattgacatc gtgtcaaaaa aacgctgaaa
    12421 acagcggcaa accaaaggta tacacatcat tttatgcaat gtatgacttt acgaaaacaa
    12481 tcggaggtga cgatatagat ctaacaaaca tagtaccgac aggaacagag ccacacgatt
    12541 ttgagccgac tgcgtctgat atggcaaaac tttcggaggc tgacatattt atatataacg
    12601 gcgtcggaat ggaaagctgg gcggacaaaa taatcgaaac acttccgcag agcgtaaaag
    12661 ttatatgcac atcggaacag ataccgacag acggaaacga tccgcatatt tggcttagtc
    12721 cgcagaatgc aaaattgcaa atgcaggcaa tatgcaacgc gttgtcagag gtggacagca
    12781 aaaatgcaca gaactatata aacagacttg acagttatct aactcaaatt gatgaggtag
    12841 acaccgagta caaaaatgcc gaacttgacg gtaagacgat atttgtaacg cacggtgcat
    12901 acagttatct
//
LOCUS       DAWACS010000003        11583 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_653_length_11583_cov_2.977620, whole genome shotgun sequence.
ACCESSION   DAWACS010000003 DAWACS010000000
VERSION     DAWACS010000003.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11583)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11583)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11583
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_653_length_11583_cov_2.977620"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2529..2538
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7116..7125
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2995 a         2451 c         2252 g         3865 t
ORIGIN      
        1 gcaccctcat cggtacatct tgcaagttta cccaaaagcg aagaattgct gcttgagttt
       61 ttacgcgatg cctcacgcgc ctttttggca gcctcacgag cacgtgacgc ggtcaaagtc
      121 ttttcgataa taatttttgc aattttagga ttttcctcaa gaaatgcaga aagcttatcg
      181 tttaaagcat tttcaacaag cgctcttgcc tcactgttac caagctttgt tttcgtctga
      241 ccctcaaact gtgcctcgac aaccttaacg ctgattactg cggtaagtcc ttcacgggta
      301 tcttcacccg ataggttagg gtctttttct tttagtaaat tattcttttt tgcgtagtca
      361 tttatgacac gtgtaagacc tgatttaaag cctgtttcgt gcgtaccgcc ttcgcctgtg
      421 tgaatgttgt tttcaaagct tagaagtgac tctgtgtatg atgttgtgta ctgcatagca
      481 acttcaacca tcatatcctc acgctgtgct tcaaagtaaa taatatcatc gtgaagcggg
      541 tctttgtttc tgtttaagta ttgtacaaat tcctttatac cgccttctgc gtgtagagtg
      601 accgtttcgg gattttcaac acgataatcg gtaaacagga tttttacacc cttattaaga
      661 aatgcctgtt cacgaagtct ttttaaaagt acatcgtaat caaattcaag cacttcaaaa
      721 atttcggggt caggcttaaa tgtaattttt gtaccggtct tgtcggtatc accgataacc
      781 tttagtttac aagtaggctt accgcgttcg taactttgat aatgaacgtg ttcgccgtcg
      841 cttacttcta cttcaagatg ttccgaaagt gcgtttacaa cagaagaacc aacaccgtgc
      901 aaaccgcccg ataccttgta tccgccgccg ccgaatttac cgccggcgtg aagaattgta
      961 agtacaactt cgactgtcgg aataccctgc tgtgggtgaa taccgacagg aataccacga
     1021 ccgttatctg tgacggtcac tgaattgtca gggtttatat caactttaat ttccgtacaa
     1081 tagcctgcaa gtgcctcatc aatagagttg tccacgattt catagacaag gtgatgcaga
     1141 cctgctgacg aagtagagcc gatatacata cccggacgtt ttcttactgc gtcaagacct
     1201 tcgagtacct gtatttgact ttcgtcatag gtggtttcaa tcttttccaa atcactcata
     1261 tttttttctc cctttatctt aattttatcc gttttgcgtg tattataaac cacgttttgc
     1321 caatactttt gaagaaacgg aggatataaa aactttcgtt tcgtcttttt ccttaactac
     1381 aacaaatgat ttcggcaaat cgtcggttga atttataatc agaccgtttt tttgagattc
     1441 ggcaagaaag tttctgccga gccttgaaac agtcgtatta tccatatcaa atattcctat
     1501 tatatctttt gtgtttacaa caatgttttc ctcaactcta aggtacatta attttcctcc
     1561 cttacgacag agccgttttt tattttaaat aacttcgtat tgtcggtgct ttcgataagg
     1621 tcggtgtccg ttgaagttat aagaacttgc ttatcgcgaa ttttctgcga aaggtacatt
     1681 cggcggttaa tgtcaagctc actcattata tcatcaagca gaagtatcgg atattcgctt
     1741 tttatgtgat gaatgtaatc ggcttgtgca attttcattg acagtgcaga tgtacgctgt
     1801 tgaccttgtg atccgtaaat tttcgcctca ctgccgttga ttgtgatgtg caaatcatct
     1861 cgctgtacgc cgacttgggc ggaggctaaa tcaatttcgc gtctttgatt tttttcaaga
     1921 tattcgtata tcgaatcttt atcatcaccg cttgccctca tacccggcat atacgatatt
     1981 tgcaattttt cgcccgatat ttcattttgt atggcggctg aaaagtcatt tatcaacttg
     2041 acaaaatcgg tgcggtattt gatgattttt tcgccctctg cggcaagctg ttcattccat
     2101 acggaaagca tcatatccga ctttgcacct tttgcacgaa gaactttgag taagctgttt
     2161 ttttgaccta acgccttgtg atagtcgatt aggcttgtaa ggtatcgcgg ataaagctga
     2221 cttaccgatg aatcgataaa ctttcgtctt gcggacggcg aacctttgac aagctccaaa
     2281 tcttcgggtg aaaacattac aacattgaga taattcataa gtttgcttag ttttgttatc
     2341 tgaacgtggt tgattttaat gttctttctg ccgtttttca taagctgcat agcggctgtg
     2401 taatcacgtt ccgaatcgtg aaaatcaagc gacagacgcg aaaaatcttc accgaatttt
     2461 ataagctctt tatcggactt tgctctgtga cttctgcctt gtgagaaaat atataccgcc
     2521 tctaaaatnn nnnnnnnnat atttgtttta ccctgtgcat tgtcgccgta aatcacgttt
     2581 gtgtacgggg aaaactcaat tttttcggaa gtataatttc tgaaattcgc aagggacagt
     2641 gaggaaatat acatttattc cgctcctact tcgacttcga ctgtcgcatc ctcaaattcg
     2701 accgtaactt tgtcacccgg acggattttc ttgccgcgca ttgtgcaaac ttcaccgttt
     2761 accgatacga taccgtcaaa aatcatatcc ttagccatag caccgctttc ggcaagtgcg
     2821 gaaaatttaa gcagttggtc aagttttata tattcggttg tgatgtttat attcattttt
     2881 tccataagac tttattcctc aataaattat taattctgta atcttgttac agatgtaaca
     2941 ccttgaattt gaactatttt cttttttaga agtgcaagat cggaggtgtt tctgatttcg
     3001 acaccgattt gtataattgc gatacctttt ttgtttacat acgcatttac cgatttaaaa
     3061 ggtattttaa gtgacgaaag aatgtttgtg atttcaagca aaataccgtc gatgtcaggt
     3121 gcttccacct gcaagttagc aatgtatgac gtactgcgtt catcgtccca ccatacgtca
     3181 ataaatctgc ccttgtcttc ttcggacatt gactgcggat tcatattcgg acagtcgcgt
     3241 ctgtgaacac ttacgcctct gcctttcgtg ataaagccga caatgtcgtc accggcaacg
     3301 gggttacagc aacgtgcaag acgtacaaga cagttatcaa tgtctttaac aacgatacct
     3361 ttgcttgatg cgtgacgctt agccggttcg gcttgaacgg ttttgctttc ctcgttgtat
     3421 accgcctcaa gatttttctt tgcttcaaga gttttgttca gctttgtcca ttcgtcacga
     3481 agtctgaaaa cgactttttg tgcggtaagc ccaccgtatc cgacgcttgc gtataggtcg
     3541 tcaatacttt gaaaaccgta tcttctcaaa agaatagcaa tccattcttg tttgaaaagc
     3601 tgttcgtgag tgttgccgat acgtcttaat tcacgttcga taagttcttt gccgtgttca
     3661 atattttcgt cacgtctttc acgtttaaac cactgattaa tcttgttttt cgcctgtgaa
     3721 gtgcgacaga ttttcagcca gtcacggctc ggaccgtgcg tatttgcggt taggatttca
     3781 actatttcac cgttttgtac catatagttg ttcggaacga tttttccgtt tacctttgca
     3841 ccgctcattt tatatccgac ttccgagtgt attgcaaaag caaagtcaat aaccgtactt
     3901 tttgcaggaa gtgaaataac tttaccttgt ggggtaaata cgaaaacttc ttcttcaaac
     3961 aagtcgaatt taagtgtgtt aaagaaatcg tcggtatcta taatatttgt ctgtgtatca
     4021 agcagttgac gtacccattc aagttttgaa tccatatcgg ttttacctga tataccttct
     4081 ttgtacttcc agtgtgccgc gataccgttt tcggcaattc tgtgcatttc ccaagtacga
     4141 atttgaattt caaaaggtgt accgttagga cctataaccg ttgtatgaag tgattgatac
     4201 atattcggtt taggcattgc gatatagtcc ttgaaacgca taggaacggg agtgtataaa
     4261 tcgtgtacca taccgagtac cgcgtaacag tccgcaacgg tatcaacaat aattctgact
     4321 gcgaaaaggt cgtaaagttc gtcgattgtc ttgttctgcg tgtacatttt tctgaaaata
     4381 ctgtaaaaat gctttgcacg tcccgtaacc tgacttttaa tattcatttg gtcaagtttt
     4441 tcacgaagta cgtccataat gtcgcgtatg tacttttcgc gttcgtcctt tttctgattt
     4501 atgctctgac gaatttcttc gtatgcgaca gggtcaaggt actttaaagc caaatcctca
     4561 agttcgattt tgatttttga cataccgaga cggtgggcaa gaggagcata tacgtcaagc
     4621 gtttcttttg aaattaaaag ctgttttgca cgcggcataa agttcaatgt acgcatattg
     4681 tgcagtctgt cgattagctt tattataaca acacgtatat cctttgccat tgcaagaaac
     4741 atttttctga ggttttcgac ctgctgttcc tcacgggtgt tgtatttgat ttttctaagt
     4801 ttcgtaacac cgtcaacaag ttccgcaacg cttttgccga atatcgtttc gatattctca
     4861 taagtgtaag gcgtatcctc tacaacatcg tgtaaaagtg ccgccgaaat tgccgttgca
     4921 tcaagcgaaa gctgtgccgc aatatatgca agctgaacag ggtgctcgat atacggtgca
     4981 ccgctgcggc ggaattggtg actgtgagca gattttgcaa gcttataggc aacgtaaatc
     5041 atatctgtgt tggcattcgg gctgtaaccc ttaaccatac cgataatttt atctacaagt
     5101 acatctgtat catcagcgtg ttgaggtatt ttttccggcg aaagaattac cggctctttt
     5161 gcttcgggca tatgctcact tcctttttat gtcttttaaa attagttgta cggtttcatt
     5221 cccttgataa tggttaatat ccatttggaa tgctatattt acagtatcgc cttgatggat
     5281 aaattccgca aattcaccca ttccgaaacc gatacagttt attgtctgat tatttttcac
     5341 tatgcgaagt cttaaatgct tgttatccgc accgacaggg gcgatattca taacctgtgt
     5401 gtgtgcaagt gcgaatacgg gcttttcgtt gttcataccg aacggctcaa gttttgaaag
     5461 cagttttgca ttttcaagag ttacggaacg ctctgaaagc ggacagtcaa tatcgactgt
     5521 cggtatcata tcctgttcgg tcaatacttc gtctgcatat ttgtttatct cttttatgaa
     5581 tgagtcaata tcggacatat tgacgttcaa tcctgccgcc actgcgtgac cgccgaaatc
     5641 aataagctgt ttttcgcaat gcgtaagtgc gtcaaaaagg ttgaaaccct ttatacttct
     5701 tcccgaacct tttccgacac cgttagtgtg ggaaataagt atgcaaggct tgtaatacat
     5761 atcacataac cttgacgcaa caattccgat tacaccttga tgccaatttt cctgtgcaag
     5821 cacgataact tttttctttt caaaattcgg atcttttgca atcagttcca atgcctcgtc
     5881 aaatatttga cgttcggttt gctgacgttc tttattttca gtatcaagta aaagtgccaa
     5941 ttctcttgca cggctttcgt catttgtaag cagcagttca acggctgttg ccgcactgcc
     6001 gagtctgcct gcggcattca atcgcggcgc gattgaaaaa gcaatagttg acgcgcttaa
     6061 aggtttatcc aaaacacctg caacttccat tactgcacga atgcctattc tttttggatt
     6121 gtgcaaaatt tttaagcctt tatccacgat aactctgttt tcgcccaaaa gcggtacaac
     6181 gtcggcaata gtgccgagag tagcaatatc gacatattgg ttaaacacat ctgtggtttt
     6241 aagtccgttt ttaattgcaa gagccaaaat aagcttgaaa gccacaccta ctcctgcaag
     6301 tgcgtcaaac ggatagtcgc agtcgggtct tttcgggttt aaaatagcca ccgcggctgt
     6361 cggaagtcgg tctttgcaag tgtggtggtc ggtaataata acgtccattc cctgaagttt
     6421 tgcaaattcc acttcaccga tagcggttat accgcagtca acggttatca taagttttat
     6481 accttgtttg aatagcttgt ttacagccat gatgtttata ccgtatccct cacctttgcg
     6541 gtcaggtatg tagtattcga catctgcacc gagtgaaagt aaaaatttgt ataaaagtgc
     6601 ggttgaagta attccgtcaa cgtcatagtc accgtaaacc gcgatttttt cgttgctttg
     6661 aattgcgttt gttatgcgtt cgactgcctt gtccatatca agcataagca ttggatttat
     6721 aatatcagcc attgattttc tcagataagg tgcaatatcc ttgctttcaa ttcctctgtt
     6781 taaaagtatg gttgaaataa cgcggggaat atggtgttcc ttagatattt cgtcaatggt
     6841 ttcttctttg atttttttac tttttatatt ccaaatctta tctatcatat tttactccaa
     6901 aacgcaattt tttttacata aatactactt attattataa cattttttta agaaaaattc
     6961 aagtaaaaca ggcttttttg aataaaataa tcaacaatgg ttaaaataga atttgaagaa
     7021 gggagtgaat ataaatgaac agtaaattca ataatcgcaa taaccaaaac caacagcaga
     7081 accagaacca agatcagcaa caaaaccaac agcaannnnn nnnnngcgga aaaattgcaa
     7141 tgccgtattt ttttgttttt attttttcag agttccaaga cttgcggctt taagataagc
     7201 gttaataaaa ccgtttaagt cgccgtccat aaccgcaccg atattaccca tttcaaaacc
     7261 tgttctaagg tctttaacca tagtgtaagg ctggaataca tatgagcgga tctgactgcc
     7321 ccaaccgtta tccatctgtt cgcctttgat gtcggaaatt ttttctttct gctgttgctc
     7381 ggcaagctcg acaagctttg ctttaagcat tttcattgcg taatctctgt tttggtgttg
     7441 agaacgctgt gtttgacaag atgttacgac acctgtcggg atatgtgtaa gacggactgc
     7501 ggaacttgtc ttgttgatat gctgaccgcc cgcaccgctc gcacggaatg cctccatctt
     7561 aatatcttcg ggacgaatat caacttccac gttgtcgtca agttcgggca taacttcgat
     7621 tgatgcgaat gaagtcatac gcttgccggc agagttgaaa ggtgaaattc tcacaagacg
     7681 gtgtacacct ttttcggctt taagatagcc gtaagcatta agaccgtcga tttctattgt
     7741 acagctttta ataccagcct cgtcacccgg aagcgaatcg agaacagttg cggtatagcc
     7801 gtttttctgc gcccacattt gatacatacg gataagcatt tcgcaccaat cctgtgcctc
     7861 tgtaccgcct gcacctgcgt ggaacgtcat aattgcgttg tttttgtcgt aatgaccgct
     7921 caaaagggtt tcaagcgtca ttttgtcaat atccgcctcg atttcttcag ttgccgaaac
     7981 aacatcgggt aacatacttt cgtcgttttc ctcgtttgca aggtcgatta aaacaagtgt
     8041 atcttcccat tttgaaacaa ggctgttaaa tgcctcaacc ttgtctttta attgctttgt
     8101 tttttgaaga actttctgtg aattatccat atcgtcccaa aatccgggct gggcactttg
     8161 ttcttcaagt tttgttatct cactttccgc acctgcgatg tcaaagtgaa tccctcaaat
     8221 cgtttatgct gtcttcaaag ccttgcagct ttagtctgta ctcatcgtac tctaacacat
     8281 ttatcacttc tttcgtttta tttgttaatt tccgtaaatc ttgtgaattt catcggaata
     8341 agtaccgtct tgattataca cataaagcgg cggcaaaagc tttaattcct caccgccgtc
     8401 acgcataccc tcgattaata ccatattcgg tactttatca tatgacggat ggacgaaacg
     8461 catacgcttc ggctctattt tgtatttacg catggcacac ataatgtcta caagtctgtt
     8521 aggtctgtga atcataaaaa atctgccttt cggaataagc agttttgcac tgacgtttat
     8581 aacatcatca atggtacaca taacctcgtg acgtgaaatt gattttgtgt caatatcatt
     8641 ttgattaccg ctgccgcact tcatatacgg cggattgcag gttattttgt caaaactgtc
     8701 acgaccgtat atagaaacgg cgttttttaa atcgccgcat tttatggaaa tacgatcttc
     8761 aagcttgtta tgttccacac tgcgctgtgc catttgtgca atatcttcct gtatttcaag
     8821 accgaaaatt ttcggtgtgt ttgttcttgc ggataaaagc aagggtacaa tacctgtacc
     8881 tgtgcataaa tcaagtgttt ttttagacct tgtttccttt gcaaaatcgg ataataaaac
     8941 agcgtcaata ccaaacttga aaccgttttt cttttggatt atgaaaagac cgttttgcaa
     9001 atcgtccaaa cattcatcgt tttttatcat cagtcctcgt ttgcaatatt gtcaacaacc
     9061 gcaaagctga tttcgcctgc tgccgcaact tgaccgccta ctgttgcttt tacgtttgct
     9121 ttacccattc cgtgacggta agtaaccaaa tcggcgtgaa gctccaatgt atcgcccgga
     9181 actacctgac ggcggaattt gaaattgttg atacctgtga atacaccgag tttacctctg
     9241 ttttcaggca ttgacaaaag cattacagca ccgacttgtg caagtgcctc tgtaataagt
     9301 acgcccggca taattgggtt acccgggaag tgacctgtaa attgaggttc gtttgccgtt
     9361 acgtttttga tacctgttat gcttttgcct tcttcaaaat caacgatttt gtctataagc
     9421 aaaaacggat aacggtgcgg aagaattttt tggatttcta cgtttgttag tgtgattggt
     9481 tcgtttttca taattaatat atcctctctt tttttaatct ctgatgtatg cttttttgtg
     9541 gaatatatga gtcggttcgg taactgtgtc gatattgtca agtgcaattc ccgcaccttt
     9601 tgcaacgcag tcgattatat ccggtgcaag caccactttg acgccgatgt cgtccgaaat
     9661 acgcttgtcc aaaccgtaaa tcaaagaacc gccgcctgtc attattattc cgtcttgtat
     9721 aatgtcaccg tgaagttcgg gaggtgtttc ttcaagcact tccttaattc tctcggtcat
     9781 attataaaca aaatcatcaa atgcggggta taggtcgttt gaagaaatag ttacttcttt
     9841 cggaagcccc gaaagtacgc tgataccttt tgctttgcat aacagctcat ttgaacgcgg
     9901 tataacaccg ccgatttcca ttttaattct ttcggctgtt cgcggaccga taatcatacc
     9961 catttctttg cgcatataac gaataatcgc ctcgttaaat tcgtcaccgg cgattttcag
    10021 cgaacggctg acaacgatac cgccaagcga taatactgca atgtcggttg tacctccgcc
    10081 tatgtctaca accatagtac cgtgcggacg tgaaatatca aaccctgcac cgagtgctgc
    10141 cgcaacaggt tcttcgataa tgtaaatatc ttttgcaccg acatctcttg ccgcctcaat
    10201 tacggctctt tgttcaacgt ccgtaacacc gctcggaacg cacatcatta tgcgtgggtg
    10261 accgattgtt cgtgaaaata tttttgaaaa gaaatgttta atcatttcct gtgtaagcgt
    10321 aagatttgaa attacaccgt caataagcgg acgaacggcc tgtatatgcg gcggagttct
    10381 tccgagcatt gcgagtgcgt ctttgcctac ggcacaaact tcgtttgttt tggtgtttac
    10441 cgcgataacg gtcggctcac gcagtacaat gcccttgttt ttgatataga caagcaccgt
    10501 cgcagtgcct aagtctatac ttatatcatt attgaacata tttctgctcc taaattaaca
    10561 aaatcggact atacgcaaaa tgcttatagc catatactat tattatatcg tattattcgt
    10621 tttaactcaa cctattttca gaagtttttt cgcatttttg taaaaaatat tgttacaatc
    10681 ttcctctgaa agaggtaagg tagataaatg ttttgccatt cgaccttgct tttcccaagg
    10741 tgaatctgtc gcgtgaagta ttttttcact gccgtgattt ttgattatgc gtaaaagctg
    10801 ttcatctgtg ataaagttaa gcgtaaaggc tgtgtcaaaa tatatcggtg tgccgacaag
    10861 gtatttttcg acatcgtccc ataacttcca gccgcccaaa tgtgcggcaa cgattttgtc
    10921 gcctttaacg tagtcaagaa cgtgagaaag cctttcggga gtacagtgaa caggcggatc
    10981 gatacctata tctttgcccg aatgaagtac ggttataagg tcgagttcct cacatttttt
    11041 cagaatttgt attgattctt tgctgtcgat gtaaaattgt tgatattcgg ggtgtagctt
    11101 gataccttta agcccttttt ctttgatgcc atacaaaaca ctttcccaat cagattgcat
    11161 agggtgcaga ctgccaagtg aataaatacc gtcaatgccg tttattgatg cggcgaagtt
    11221 atttattgtt gttgattgct ttacgttcgt ggcaatcgga agtacaagcg atatgtcaac
    11281 gttgttttca cgcattgact gcttgagtgc gtcaagtgta ccgcgtagtt cggctttgtg
    11341 tggcctgtat tcttctttga tgttcgattc aagtaatttt atcgttttgt ccgccatttt
    11401 gtctggaaaa atatgtgtat gaaaatctgt cagcatttta aatatccttt cgtttgattg
    11461 attatattat agcataaaac ggtatataat atcaagaaaa aagaaccgat tttattgtcg
    11521 gttctttagt ggtgttattt atgaatttcc ttcatttctt cgtgcatatt tgcggcattt
    11581 tga
//
LOCUS       DAWACS010000004        11581 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_655_length_11581_cov_2.179247, whole genome shotgun sequence.
ACCESSION   DAWACS010000004 DAWACS010000000
VERSION     DAWACS010000004.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11581)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11581)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11581
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_655_length_11581_cov_2.179247"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1699..1708
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3879..3888
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6149..6185
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8109..8118
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3716 a         2404 c         2162 g         3232 t
ORIGIN      
        1 ttgaaattac cgcaggacga agttgttcgt cttaccgaaa atgctcttaa acgcacaggt
       61 atttttgatt taaaagacaa acctccccat gcattaagtt tcggtcagaa aaaacgtgcc
      121 gcaatagccg gcatacttgt tatgtcgccg gaggtaatca ttcttgacga gccgacggca
      181 ggtcttgacc caatgggcgt aagtgagctt atgcaccttc ttcgcgacat atgcgaaaat
      241 gagggtacga caattataat gtcaacgcat gatattgacg ttgtgccgat ttattcggac
      301 tatgtttacg ttataaatca cggtaagtta cttacagacg gtactcccga agaagtattc
      361 tcaaatccga cgcttttgcg tgaacataat ttaagattgc cgagaattgc acatctactt
      421 gaaattttga ataaatgtga taaacttaat gtagacttct caaaagccac tatcggcaaa
      481 gcaagagaag aaattttaaa tcttataagg ggtaaataac tatggcaaaa ttctattccg
      541 tcggagtcgg tgcaggtgac ggttcatata taactctcgg tgcgcttcgt gcattggaac
      601 aggctgatat aatcgcagtt cccgttaaaa aatacggaga aaaatcaact gcacttgaaa
      661 taatccgcaa ggaatttgat acggataaaa aagaaatttc agaacttgaa ttccgcatga
      721 gtggggacac taaaatcaga gaagagtccc gcagaaattc agccgaaaaa attatatcgg
      781 cgttaaaaga cggaaaagat gttgcaatgg ttactctcgg tgacgtttcg gtttacagta
      841 cctgcacata cgttcacaac gcagtgaaaa atgcaggctt tgaacttgaa attatacccg
      901 gtataacttc attttgtgcc gctgcggaca aggcacagat aagtctttgc gagggaaacg
      961 aatcggttgc gattattccc tcgcttaaat caaatttact tgaaaaatat attgccgatt
     1021 ttgatacggt tgtgattatg aaatcgggca acgatacaga tgtaatatac gacattttaa
     1081 aaaagtacgg acttgaaaac aacgcaatcg tttcaagctg tatcggtatg gaaaacgaaa
     1141 ttatcgaaaa aatacaaaaa ggcaaatcat agggttattt tacaaccgtt attgtaagaa
     1201 aataatcaga aaggttatgg gataatggaa ctgcttgcag tgagtatcag ccacgaaaat
     1261 actccaatat caattcgtga gaaagtatct tttacaaaga aaaaacaggc tgaaatttta
     1321 aagtatataa aacagaatat cgctgaagaa tgtgtgcttt tgtccacttg caaccgttgt
     1381 gaattttatc ttgcggggta taatatgaaa gataaattca ttgactgttt agtgtcccta
     1441 accgacgaat ttgttaaaaa gtacataagc atatatgagg gcgacgattg ttcgcgtcat
     1501 cttgcactta ccgcgtcggg gcttaaatca atgattctcg gtgaggatca gattttaggt
     1561 caggttaaag acgcgcacga atttgcaaaa gaacatttgt gctgcggaaa atatctcaac
     1621 actcttttcc gtatttccgt aacgggtgca aaaaaagtta aaacggaaac gcttttatcg
     1681 aaaactcctg tttccgcgnn nnnnnnnnca ataaaacagt gtcaaaatat tttggggaca
     1741 ctaaatagca aaaacatatt aattatcggt gcaagcggaa aaacaggctc tatcgttctt
     1801 aaagatttac tgtcccttga cggtgtaaat atttatgcca cttcgcgtac gcatgacggc
     1861 attataaacc acattgacgg tgcgattacc gttgattacg acaagcgtta tgataatctt
     1921 gatatgtatg acgccgtaat cagtgccacg tccagtccgc atatcgtact tgaaaaaagc
     1981 agagcaaaaa acgcaataaa gacttgtaaa aagcgagttt ttatcgacct tgcagtacct
     2041 cgtgacattg aggtattcga ggatgaatac acagtttaca aaaatattga cgatttaacc
     2101 gaaatcgcgg acaacaacaa cagaattaaa caacaggaaa ttatgaaagc cgaaaaaatt
     2161 cttcaaaaat atattgatga atttcgtata tggaaacttt tttccgaaag cgaaacatta
     2221 ttcaaatcgg cagagaataa aattgaaaac gagagcgaaa agaacacttt tcgccataaa
     2281 atatataatc tgaaatcaga caacaattat gcaaaattca gtgaatttat tattttgctt
     2341 agggagaaat taacgtgagt ataataaaaa tcggctcaag aaaaagccaa cttgcaatca
     2401 aacaagcaga ggctgtaatc aaaaaattga aagagcattt tccaaacgac acttttgaaa
     2461 ttgccggcat ttcaacaaag ggtgaccgtc agcttgataa gtcgctccaa agtttcggcg
     2521 gaaaaggtgt ttttataaaa gaaatcgaaa ccgcactttt aagcggtgaa atagatatgg
     2581 cggtgcatag tgcaaaggat atgccgacag aaatttgcga tggtcttacg ataagtgccg
     2641 cacttcttcg cgacgacaga agtgatgttc ttgttcattt tggggacact aaattatccg
     2701 acataaaaat aatcggcaca ggcagtgcaa gacgtcaggc acaggctgaa aaactgttcc
     2761 cgaacgctgt ttttaagcca atcagaggta atatcggcac tcgtcttgaa aaagtcaaaa
     2821 acggtgaata cgatgcggtc attatggctc ctgccgcaat aaaccgtctt gccgtttccg
     2881 acgttaaaat cgaaaatctc ggcgataatt tcatatgcgc cgcaggtcag ggaattcttg
     2941 ccattgaaac tgccgtcggc aaagccgata aatacacaca agcgataaat gatgaaatcg
     3001 caatgaccga attaagatgc gaacgtgcat ttttgcagta cacaggcggc ggctgtcacg
     3061 caccatgcgg tgcgtcggct gaatttaacg gtaaagaaat taaaatgtgc acatttttca
     3121 aagaaaacga caaagaaatt tatcttgaaa tgttcggaaa cgatccgata ttactcggca
     3181 aaaaaatggc tgaaataacg cttgataaaa ttaaggagta aatcaaatgg tatatcttgt
     3241 aggtgcagga cccggtgaca gcagattaat tacgcagaaa ggtcttgaat gtataaaaaa
     3301 agcggatgtc ataatttacg accaccttgc aaatccgact ttattgagct atgcaaaaaa
     3361 cgactgtaaa ttgatttatg ccggaaaaat cgcaggcaat catcacatgg tacaggagga
     3421 aatcaacaaa acccttgttg aacacagccg tttcggaaat gttgtacgtc tgaaaggcgg
     3481 cgatccgttt atttttggtc gcggaggtga agaaattatc gcactaatcg aaaataatat
     3541 tgactacgaa attgtacccg gtatttcttc ttgttacagt gccgccgaat attgcggtat
     3601 tccagtcact caccgcggag ttgcaacttc gtttcacgtt ataacaggtc acgaaaaagt
     3661 cggaaatgaa acggttaatt acagtgcgct tgcaaaactc agcggaacgc ttgtttttct
     3721 tatggggctt tcgtctgccg aaaatataag caataagctt attgaaaacg gtaaatcggt
     3781 aaatacacct gttgccgtaa tttcaagcgg cacaacgccg cgtcaaaaat gtgttacggg
     3841 gacactaaat aatttatctg cattggcaaa acaaatgann nnnnnnnnca ccgttaatga
     3901 acaaaagcat aaaaaatgcg gcgaccgatt ttgacataac ggagctttct ttaatcaaaa
     3961 cagttccgat taatttcgac ttgttttcaa aagcggatat agcacacttt tcacatatag
     4021 tatttacaag tgcaaacggt gttaaaatat tttttgaata tttacaaaaa tcaaaaaccg
     4081 atatacgcac tttaagggac actaaattcg ctgttgtcgg caaaaaaaca gccgacgcac
     4141 ttgcaagtta cggcatatat gccgacatgg ttcctcaaat tcattcggga cttgagcttg
     4201 ctcgcctaat gtgcgaaaag tgcagtaaaa acgacaatat tcttttaatt cgagccgaaa
     4261 acggtgcaag caccatactg aatattttaa gtgaaaataa tattaatttc accgatatgc
     4321 acctttacag aacagaaacc gacaactcaa aacaggaact tttgaattta tgtctaaacg
     4381 atactgacta tgttatatta tcaagcggtt ctgccgcaaa ggcattttcc gaaatggcag
     4441 acacgtcaaa tataaagctg atttcaatag gaaacgaaac aacaaaatcc gccgaaaaat
     4501 gcgggcttaa aatttataaa acagccgaca atgcaaccgc agaaagcatt atcgactgta
     4561 tcaggaggga cactaaatga tcagacgacc aagacgactt cgtgtgacaa aacaaattcg
     4621 cgaccttgta cacgaaactt cgattgacaa aacagactta atatatccga tgttcataat
     4681 tgacggcgaa acagaagata ttgcgtcaat gccgaacatc aagcgatact cgcttatcga
     4741 cctgccgtcc gcacttgacg aagtggtttc ggcaggtgtc ggtgcggtgc tttactttgg
     4801 tattccgaaa acaaaagatg aattcggcag tgaggcatac aatgaatttg gcattgttca
     4861 acgtgcaatc cgccttacaa aagaactgta tcccgattta atcgtaattg ccgatatttg
     4921 tctttgcgag tacacttcgc acggtcactg cggtgttatc gaacacgact gtatcgtcaa
     4981 cgacaagact ttaccgcttt tagcaaaagc gtcggtggct tgtgcaaaag caggtgcgga
     5041 tattgttgca ccgtcggata tgatggacgg acgagtaggc gcaatacgtc aggcacttga
     5101 cgaaaaccgc tttgagaacg tacttataat ggcatacagc gtcaaatatt catcatcatt
     5161 ttacggaccg ttccgcgatg cagctgacag tgctccgtca tttggggaca gaaaaagtta
     5221 tcaaatggat ttcagaaata aaaaagaggc aattatcgaa acacagcttg acattgacga
     5281 aggtgcggac ataatcatgg tgaaacccgg tatgccgtat cttgacattg caaaaacggt
     5341 cagcgataat tttagtgtcc ctcttgcaat atatcatgta agcggtgaat actcaatgat
     5401 taaagccgcc gcacaaaacg gttggattga cgaaaagtca atcgtccttg aaagccttac
     5461 cgcaatgaaa cgtgccggtg caaaaatgct tataacctac tacgcggtcg aggctgcaaa
     5521 gtggttggag gaaaactgat gtttccgata atgctgaata tacgcggaag aaaatgtacc
     5581 gttgcaggcg gtggaaatgt tgcggaaaga aaggttaaaa cgcttttaaa atatggtgca
     5641 gacataaccg cagtaagtcc tgtttttaaa gacggatttc caaacgtcaa acgacttgaa
     5701 aaagaatatt cctcaagtga catagaaaat tcttttcttg tcatagccgc aacagacaac
     5761 aaagaagtaa atcaaacgat ttacaatgac gcaaaaaaac acaatatttt agtgacccta
     5821 tctgatgaca cagagcattc cgattttata ttaccgtcat caaaaaacta cgatgacata
     5881 acgatttctg tttcgacaaa cggcaaatca ccggcacttg cgaaaaaaat cagacaaatt
     5941 aaatcaaacg atttagaatt ttacgcaagc attttaccta taatagagaa atacagaagt
     6001 attttaataa gcgaaagcag ggacactaaa aaagaaatat taaattttat ggtttcagat
     6061 gaaatgcttg aaattgcaaa agaaagttta gaattatatg aacagaaaat aaaggagaaa
     6121 atatgaaaac tgcaatactt gtcataagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnatgat tatcaaaaag ctccgtgaac gtgacggaat agaaattgat actgtagata
     6241 aagcacttga aaaattatat aaaagcggtt acacagatat tttttgtgtc cctactcaca
     6301 ttatgtgcgg tattgaatac gacaaagctt gcagaatgat tgagaaattt aacgacaaaa
     6361 tgaaaatcag aatttctcgt ccgcttgtta ccgaaacgag tgactattac aacattgttg
     6421 atattttcaa acacagactt accgacaaaa acagacttta cgtttttatg ggacacggca
     6481 gcgaccattt tgcgaatgcc gcctaccctg cccttgatta tcactttaaa aatgccgata
     6541 tgaacaacgt atttgtcggt actgttgagg gttttcccga ccttgaaaca gtcatctccc
     6601 acgcaatgaa aaccgatata aagaatgtaa cactactccc gtttatgctt gtatgcggtg
     6661 accacgcgaa aaatgatatg gctgtcgaat ggaaagaaga atttgaaaat aacggtttca
     6721 atgtcgagtg tgaccttacg ggacttgtta ttttcaaagt ttctgctttt ccgtattttc
     6781 taaacagtgt tttaccctct tgttgagttg tttcccagcc ttttgacgtt gtacgcgcat
     6841 taccggcaac ttcaaccgtc atcataccgc cgtcacagaa tttaatcgtt gcacccttat
     6901 cattctttga aatttttgca ggtacattca ccgaagtgat atttatatct ttattttcgc
     6961 cgttctttga aatctttatg cccttatcaa gcgacgcata aatacagtta tctttcttat
     7021 atgcaacact tgcgtctatg ttaaattcat caaccgtaac accgtcggca aaaatcactt
     7081 ttacaccggt agaattttga taattcttat cgtacagcgg aatatcctca ctttttgccg
     7141 ccgccgacaa atacaaagta tcattatccc actttgcact gaccctgctg ttatatgccg
     7201 ccgcagtcat ctttgcaagc tgattttcct gtacgaaatt atagccgtaa tcatctacaa
     7261 gcgtatcaac ctttttaatc ttctcctcct cgctgtcctg ctcacggtaa acgtaatcgc
     7321 agtgattgta gaaaagaata ggcacttcat actttgcgga aatcgccgaa aattcgctat
     7381 ttccgttcgg tgtatttgac ggttgaagca taagctgacc gttatcgaga tagaacggaa
     7441 caagaattga attttccgcg cttacttccg gtacggcgat actgttcgga gtctgtgaac
     7501 cgctgttcca ataaagtccc gcatcaaact gactcttgta cgttccgtca taaccgctca
     7561 tattatcaaa atgcgtgtca taaccgattt tacttgttct ccatgtatgc tgatttacac
     7621 cgcttcctgt ccatggaagt ccgtatgact caaacgcatt tttttgatat tcaagctccc
     7681 tttggtcatg cgtactgtcg gcatattcat ttgaaagtgc gtgtacaccg acatagattc
     7741 ctctgtcctt aaagccgtca agctgtttct gcaatttttc acgatacgga aaataatcgc
     7801 tgtctatagg aacagccata ccgtattcaa gtgagccgac aaccaaatct ttgttgccgt
     7861 caccgtcaat atcataaaat cgcggtacac tgttcgcacc gaatttcaga ttattattac
     7921 ccttataatt gctttcattg ccttcaatat aaccgttaaa aacattattt tcaaacttcg
     7981 cgatatatcc ctcaaacgta cctgcgtaaa gctcattcac gccgtcaccg tctgcgtcgg
     8041 ctatacaagg cgaacaccaa ctttgacctg tatcaacggt ttcgtaagca ttaaacaaaa
     8101 cactgtacnn nnnnnnnnac actgtactca cccatatcac cgtaatatat tctcatttcg
     8161 ccgtttcgtg agccgaccgc catatcaagc acgccgtcac cgttcaaatc accgattgac
     8221 ggcattgcgt cggtaagtcc tgtttcaaga atgatgcctt gatcgtcaag caccataccg
     8281 tcagatcttt taaagcaatg gatacttccg ttttcatcac ctgtaattat ttcatcaaca
     8341 ccgtcattat caatatcggc ggttgtaggt gatgaatatg caccgaccga aatttgattt
     8401 ccgtccatat cggtaaacat agtcgcaacg tcaagttcgt aattcgattt cattcccgta
     8461 ccctcgaaat agtacagttt tccgtctgca cttccgcaaa tcaagtccat cttgccgtca
     8521 ccgttaaagt ccgtcggaca cggataagca cgctttatat aatcgtggct gtcctcaaaa
     8581 tagcttattg tgttatcgtc ataatcaagt gacagccatt cctttgcggt tacaaagtgt
     8641 gttcccgatg aatacgcatt ttcatacgga tccattgcaa attgaccgtt attattcagt
     8701 gcatacgtca cactttcggc acgtctgaac caaatatacg gatttcttgc aagcgtgaat
     8761 gacggtactt gaccgtatct ttcacacatt tcctcaaaaa tttccgcgga gccgttatct
     8821 atacccgtaa tatcctcata gtgaagctcc cacgaagcaa caggttttgc aaagctgccc
     8881 aaaacgtgtt caacggcata tccgtaattt ttaagcgaaa caaattccgc aaaataatct
     8941 ctgagcaatt tatttgcacc gaccgttgtt gctgaaaggt attcgcctgt atcatcggga
     9001 gaaagattgt ttacggaaaa tgaattcggt aaaagcggat tggtgaagaa cacataaccg
     9061 tttccgtact gatttaccgt ataaattccc acattatcct ttgacgcaat acattccgct
     9121 gttgacggaa caacgccgac tccgtaattt tgatttacaa gcacatcatt ataatcggca
     9181 taatttctgt acaacatcga aaaatcagac aaaagcgact gaattttctt tattccttta
     9241 tcgctgttct cgggataaat catatcaacc ggacaagaat caatcgttac aaagtcctcc
     9301 gcaccgataa attccttatc aaatacgtta tacacatcat tgtcaaggaa aacacttccg
     9361 ccgtttgata catattcctc aactgcactt gcattaaaac cgcctgtttc aacaacgctt
     9421 ttatcaatgt acaaaacatc ataatccgac aaatcaccga tatttgacgc gtcgatttta
     9481 tctgctgtca atgttgcaac ggttgattca tttaaactgt ttaaagtgtt ttttgccgta
     9541 acactgtccg aggacgaaat taccgctgta gacaatgtat cgggattttt tacctgttca
     9601 tttccgcacg ccgtaagaca aagcactgca agaaatattg gtataagttt tttcataaac
     9661 tttctccttt taaaatgcct catatataaa tgtaccgtga ccggtaaaga atatacatat
     9721 taaaatgaac attgcaaggt atattaaaaa tgttcctatt gtttttaatt tttgtttcat
     9781 ataaagatcc ttccaaaaat tgttcacagc caacttgcgg tgccgtacac tcgtactgcc
     9841 cccggttgca tgggcgagca gcagactatc agtctgcgac cagcccaagc aaaaagtagt
     9901 gctgtgggcg atttttctac aaccattcat ctatcatttg agtgaagata gctgaaccca
     9961 cgtcataatt caaatgtccc gtatcgtaaa aacattcggg tgacaaagca gtttccatat
    10021 caagcacttc cacgttatac ttatcaaata cttcgtttgc catagccttg acttcaccca
    10081 acaattcagg ctgttcaagt ttcgtattcc acggcatcca aaccgcccta acccttatat
    10141 tattatcatt acagtaattt ataatcttct caagtgcggc cacattcttt gaaaacttct
    10201 ccgtcggaag attgaaaaat tcgtcttgat aattttccat agtcttttca agcattttat
    10261 ccgacattgc gccatgataa atatgctttt cctgcggaag atatttatac ggcaaaggac
    10321 tttcgccgtt taacagctta tcaaagccgt ctgttatcat aggataaaat ctgtcacgct
    10381 caaaatacgc atacggctca tacaactttt tatgccatat atatgtcgga ttggtttcaa
    10441 actcatcata caacgttaaa taattcagtc cgataacaag ttcagagccg atatttatat
    10501 gcttcttctc aatcatttcc cacaagtcgg taacaacacc gtagtcaaga ccgagattga
    10561 catatccttt actgctctcg tcctccatat atgccgaaat aacaacgctg tttccggaaa
    10621 acaccttatc ccaaactttt tcggggtgtt tatactgcgt tatttcaaaa tcgtttttca
    10681 caaaataatc actgttcact atgaaattgt tataggtcag tgccaaatca ccgaccgcta
    10741 ttgcaagtac aatcgcaatt attgttacaa tctttttcat attcttataa atcctttaag
    10801 tatctcaaaa aactgtgcag gtgttacggt aaacatcaat gtattaaatg caaaaagcat
    10861 atttactata atacatcttg caatatagac aggctttttc atcttacgtt tttccgcctt
    10921 tgtaagtccc aaaaggtttt caagaccaag acacaagccg ttataaatac cgccgataac
    10981 gtacacccaa gtaaaaccgt gccacatttc cataacgtac attacaagaa agcccattcc
    11041 ggctgaagca agttttccga gttttttacc gtttaataca acaaaaatat gttctctgac
    11101 ccaatcggaa agagttatgt gccagttacg ccagaactgc gtaaatgacg ccgccgccca
    11161 aggctttctg aagttttcgg gcactgtata tcccataacc gagcctacgg ctatcgccat
    11221 atcgctgtaa cccgacaagt cgaagaacag gaacagatag ctgaaaacca ctatcgcaag
    11281 gcttacatac caggaagtta aagaatgtac ttacaaagta aagtacaacc attttcttaa
    11341 acataccctt tataaatcgc tttacgcaag ttataaatct gtctgtcgtt agcggtactg
    11401 gtttattata taccttttga aaatctctgt atctgaaaat cgggcctgcc gtaaacgtcg
    11461 ggaaaaacaa catataattc acatacgtca aaaacggaat atccatatcc gtatagtaca
    11521 cgaagtacat aacgtcaacc gcttttagca tatgatacgc tattcctaca aatgtaatta
    11581 t
//
LOCUS       DAWACS010000005        11258 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_672_length_11258_cov_2.295367, whole genome shotgun sequence.
ACCESSION   DAWACS010000005 DAWACS010000000
VERSION     DAWACS010000005.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11258)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11258)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11258
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_672_length_11258_cov_2.295367"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3574..3583
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4402..4411
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5300..5309
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8106..8115
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10952..10961
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3316 a         2400 c         2000 g         3492 t
ORIGIN      
        1 tctagtaaag cggacaaaaa cataacggtt ttgtctacaa aataacaaaa actgcatttt
       61 gaataaaatc aaaatgcagt ttttttatat cttatcttaa ttgagcaccg atttgatttt
      121 caagattttt aacaacctta tcaaatactt tctgtacttc atcacctgtc aaacttctgt
      181 ctgcgcttct gtatatagca ctgtatgcaa cgctcttttt gccctcaggt atctgtttac
      241 cctcgtaaac gtcaaacaac tgcaagcttt caagcatttt acccgatgcc tttttgatta
      301 cattttcaat atccgcaaca ggaactgtct tatcaacaag cattgctata tcacgagtta
      361 cggcaggata cttaggaagt tccttaaact taatatcagt cttaatatta aggaatacat
      421 tctcaaaatc caattcggca atatatacag gagtttcaat tccgtatttt ctgcttactg
      481 cagggtgaat ttcaccgatt atgccgatta ccttattgcc ggcacttatc tttgcacatc
      541 ttcccgggtg gaatgtcggg ttatctgtta cggcctcata cttaacattc tttacgttaa
      601 gttgtgcaaa cattgtttcg catataccct taatgtcata gaaatctacg ttatcgccgt
      661 acatacccat tgtgatttta acaggttcgt tcggaagttc accgtccgtt gtcggaataa
      721 atatctttcc tatttcaaac aacttggcag acggatttct gtaattatag tttcttgaca
      781 atatatccat catacttgcg atagtagttg tacgcattac cgatgtgtcc tcgccaagcg
      841 gatttgtaat ttttacaacg tttctcattt cactgtcttt cggaatattc agtttatcga
      901 atattgacgg acttgtgaat gtgtatgtgt aaatttcgta cataccttgt gctgtaagaa
      961 gttcgttcac tctgtcctgt acctgctgac gttctgtttt cattccgcaa gtagcctcgc
     1021 ccgaaagaag tgttacaggt attttatcgt aaccgtagaa acgtgcaact tcttccgcaa
     1081 tatccgcctc accttcaagg tcaggtctga atgacggagg agttacagtc atcttgtcaa
     1141 ggtcaacctt gatttcaagt gcgtcaaaat acttaaccat atcctctgtc ggaatgtctg
     1201 ttccaaggaa cgcattaatt ttatcaggtc tgaaagcaag cggctgcata tctgttacgt
     1261 tacccataac gtcaatcatt ccgccgacat tttcaccaca gccaagtttt tctacaagct
     1321 gacacgctct ttcaacggca ggaactgtat tgttatagtc aagtcccttt tcgtatcttg
     1381 atgatgattc tgttctaaga ccgactcttt gtgctgtaag tcttacagac gcaccgtcaa
     1441 atgtagccga ttcaaaaata acagttgttg tatcatcctt aacttcactg ttaaatccgc
     1501 ccataacacc tgcaatagca acagctctgc cgccgtctgc gattacaagg tcgtttgaag
     1561 taagtgttct gtcctgttca tcaagagtct ttataacttc accgtcattt gcacgtctta
     1621 cgataatttt gttatcctgt aaatctctca aatcaaatgc gtgcataggc tgaccgtatt
     1681 caagaagtac atagtttgtt atatcgacaa tattgttgat aggtcttaca ccgcacgcac
     1741 gaagtctttc ctgcatccaa ctcggtgacg ggccgatttt aacgttttta accattcttg
     1801 cacagtatct cttacactta tccttgtcct gtacatcaac gctgattgta tcggcgatat
     1861 tttcactgtt ttcgttaaac ttaacttcag gaatagtgaa ctttttatta aatgacgcct
     1921 ctgtttcacg tgcaagaccg attatgctga aacaatcagg tctgtttgaa gtgatttcaa
     1981 attcaacgac agtttcattc ataccgaata tatccttaat atccgtaccg atttttgtat
     2041 cgtccggaag aataagaata ccgtattcag gttcataacc aagcatatcc tctgttatac
     2101 caagttcatc atgtgagcaa agcataccat aagattcaac gcctctaagc ttgccttttt
     2161 taatttttcc gtcaggaagt accgcaccga caagtgcaac gggaacgatt ataccggctt
     2221 ttacattcgg cgcaccgcag acaatctgca aaatctcgcc tgttcccgcg tcaaccgtac
     2281 acacatgaag atggtcactg tcagggtgat cttcacaggt aagcacctta cccgcaacta
     2341 cattctgaac agattcgccc atattctccc aaccctcaac tttagagcct gtcattgtaa
     2401 gtctgtctga atattctttc ggcgtaacgt ctatatccat atagtcatta agccaactca
     2461 taggtaaatt cattatattt cattcctttc aatttccgaa taatatatta aaactggtct
     2521 aagaatctca aatcattttc aaagaaaagt ctaaggtcgt taatatcata cttacccatt
     2581 gcaattcttt caagaccgat accgaaagca aatcccgaat atacttcagg gtcaataccg
     2641 cagtttgcaa gtaccttagg gtgtaccata ccgcagccca aaacttcaat ccaaccctcg
     2701 cctttacata cgctgcagcc tttaccgccg caagcgaaac acgaaacgtc aacttcggct
     2761 gacggctctg taaacgggaa atggtgaggt ctgaaacgta cttttgtatt ttcgccataa
     2821 agacggtgga taaacatttc aagagtaccc tttaaatctg ccattgtaac acctttatca
     2881 actaccagtc cttcgatttg gtggaataca ggtgagtgag tcgcgtcaac cgcatcactt
     2941 ctgtaaactc ttccgggtgc tataattctg ataggcggtt tcttcttttc cataacacga
     3001 acttggatag gtgaagtttg tgttctcaaa accgtatttt cgtttatata gaatgtgtcc
     3061 tgagtatcac gtgcagggtg attcggtgga atgttaagcg cctcaaagtt gtagtagtca
     3121 agctcaactt ccggaccttc tgcgatttca aaacccattc cgataaatgt gtctttaatg
     3181 ttgttcatta cttttgtaag cggatgaagt ttacctgttt tttgtgcctt acccggcatt
     3241 gtaacgtcga ttacttcgct ctttagcttg atttcttcaa gcttttctga aatttccgct
     3301 ttcttcgtat ctatgtggtt ttcgattaca ccgcgaattt cgtttgcaag cgcaccgatt
     3361 tgcggtcttt cttctttgct taactgtccc atacctttta gtacggcagt aagttcaccc
     3421 tttttaccga gaaactttat tcttgcttct tcaagagcat caatgtcagt tgctttttga
     3481 agtacatcaa gggcattttt tttgatttct tctaactttt ctctcatttc aaaattcctt
     3541 tcaatataaa ttaaaaaaca ctccgtccca taannnnnnn nnnactccgc ggtaccaccc
     3601 gacttccgat aaaatatcgg cactcaatta tgtataacgg cactacccgt cacagactac
     3661 ttgcaaaccg ctttcaccta tgcagctccg gaatgaaatt tcaaataaat ccttaccgta
     3721 tcggctcaca gctcaataac cgatactctc tttaggcggt gtatacctta ctttgttcca
     3781 tcattgcttt taatattcaa taataaagta ttatatcacc ttttttttat tttgtcaatc
     3841 aataacacaa aattatttaa aaggattgat ttttttgcca aaaaattata taatttatcc
     3901 cgaaagggtt gatttgaatt gaaagaagaa attttagata tattaaaaaa tgcagacgga
     3961 tatatttcag gcggacgcat aagcgaaatg ttgaatgttt cgcgaacggc aatatggaaa
     4021 cacattaaat cattacgcga aagcggttac aaaatcgaat cggtcacgaa caaaggctac
     4081 cgccttattt cctcgcccga catcataacc gaaagcggta tcaaaagcgg acttaccacc
     4141 gattttatcg gtcacaactt atttatatat gatgaaaccg acacgacaaa caatcgtgca
     4201 aaggaaagca acacctcgcc cgacggcagt gtatttatcg ccgaagtaca gacgcacggc
     4261 aaaggcagtc gcggacgcgg ttggacttct ccaagaggga taggcatttg gcattcaatc
     4321 cttttaaagc ccgaaatctc cccgcttgaa gtttcgcaaa taacgcttgt cgcaggtctt
     4381 gccgtctgca aggctatcgg tnnnnnnnnn ntcaatgatt aaatggccta acgacattgt
     4441 tattaacggt aaaaaaatct gcggtattct aaccgaaatg tctgctgaaa taaatatggt
     4501 caactacgtt gtatgcggta tcggtgtaaa tgtaaacaca gaatcattcg atgccgacat
     4561 tgccgacaag gctacttcga tgtttatcga aagcggtcac aaatatgtgc gaaacgaatt
     4621 aatcgcaaaa ttgttaaacg aatttgaata ttattataag aagtttctcg acggcggact
     4681 cagtgcaatt cttgatgaat acaaaaagtg ttgcgtcacg ctcggcagag aagtaaacgt
     4741 cattttcaaa aacgaaacgg ttcgcggaac tgctgttgac gttgacgaaa acggctcact
     4801 cgccgtacaa accgaaaacg gtattattca cgtcacttcc ggtgaagttt cggtcagagg
     4861 tatctacggt tacatctaaa atcggatata ttatgaataa attgtaaatt gttagcactc
     4921 acatctgttg agtgctaatt ttgtattgac aaatcttact ttgagtatta atatatatta
     4981 tgtaaattaa ttaaacttca aggagtgatt tatatggcaa aaggtaatat ttcagttgac
     5041 agtgaaaatc ttttccctat tataaaaaaa tggctttatt ccgataagga tattttcctt
     5101 agagaacttg tttcaaacgg ttgtgacgcg gtcacaaaat taaagaaact tgcaagtatc
     5161 ggtgaagctc aaattgacga aaacgaaaaa ttcaaagtta ccgtatcaat atttaaggac
     5221 gctaaaaagc ttgtcatcag tgataacggt atcggtatga ctgccgaaga aattgacaag
     5281 tacatcaatc aaatagcatn nnnnnnnnna cgacaaaggt tctcaaatta tcggtcattt
     5341 cggacttggt ttttacagtg cgtttatggt tgccgacagt gttgaaattg attcgctttc
     5401 atatcaagac ggtgcaaagg ccgcaaaatg gacttgtgac ggaagtatgg aatttgactt
     5461 aacagacggt gacagaacag aaagaggtac tacaataact cttaacatcg ccgaagattc
     5521 ggaagaattt cttgaagaaa gcaaaatccg tcaaattctt cataagtatt gtgcattcct
     5581 acctattgaa atatatgttg aagttcctga ggacaaacac gaggaccact gcgactgcgg
     5641 tcacgatcat gatcatgaac acgaggacga tacaccgtca gagccaaagc ctatcaacaa
     5701 cacaacaccg ctttggatga aaaagccgtc ggagtgtaca gacgaagaat acaaggaatt
     5761 ttacagaaac gtatttatgg acttcaacga accgcttttc tggattcacc ttaacgtaga
     5821 ctatccgttc aacttaaaag gtattttgta cttccctaaa atcaatcatg aatttgcagg
     5881 tcaagaggga cagataaagt tatacaacaa tcaagtattt gttgccgaca acgtaaagga
     5941 agttattcct gaatttctaa tgcttttgaa gggtgttatc gactgtcctg acttgccgct
     6001 taacgtatca agaagtttct tgcagaatga cggttatgtt aagaaaattt cttcacatat
     6061 tacaaagaaa gttgcggaca agcttacaag tatttataat aacgaacgtg aaaactatga
     6121 aaagtattgg gattatatca atatattcat taaatacggc tgtttaagag atgaaaagtt
     6181 ctatgaaaaa gttaaggatg taattattta taaggatatt gacggtaaat atttgacgtt
     6241 ggacgaatac cttgacggca aggaagaaaa agacgtttat tacgtttctg atccgcaaac
     6301 tcaatcgcag tatatcaata tgttcaaaaa tcaaggactt aatgccgttg tacttccgtc
     6361 aatgatggat acacatttta tttcacttgt tgaaatgaaa cagactggtg ttaaattcaa
     6421 acgtattgat tccgccataa atgacatttc ggacaacaca gaaaaagacg attcaactaa
     6481 agaacaagaa gaaaagttga ttgaaaagtt caagaatgaa atcaaggacg atacattgaa
     6541 aatcgaagtg cagtcactta aagacgattc aatccctgcc gtaattttgc ttggtgaaca
     6601 gtcaagacgt atgcaggaga tgtataaggc ttacggtcag caaatggcag gtatggctga
     6661 tatgttccac gatgaattta cacttgtact aaattcaaac aactcactta tcaagaaaat
     6721 agatacatta aatgacgagg acgcaaatct tgttattgac catgtttacg acttagcgaa
     6781 aataagccac agtcctttgc cggcagaaca aatgacaaag tttatcgaac gctccaataa
     6841 attgcttgaa aaactgtttt aattaacagt tttttcttcc atcgtcaaag agggacacta
     6901 aatttcagtt tagtgtccct cttttaatat gtttaataat tcttctattg tatccacagt
     6961 ataatccggc acttcaaatt catttttagg tgtgtttaac ttgtgaatac cctgttttac
     7021 gcatacggtt ttcataccca actttttagc cggtgcaacg tcattatcca ccctgtcacc
     7081 gaccatcaca gcattttcac ctttacagtc ggctttctca agtgcttttt gaaaaagtct
     7141 tatatcgggt tttgatacac cctcctccgc agaggatatg acaaggctga tatactttgc
     7201 caagccgtac ttttcaaggc gtttttgtgt ccccaacggc tgattggcta taactccgat
     7261 tttgtactta ccatacagtt gtgacaaaac attttcagaa tattcataag gcatttcaag
     7321 ttcactgtgc cattctgccc tattcaatcc cgatataata acagcgtcat catacggaat
     7381 taaatttttc aaagcacagt cgtccattaa tttataaaac tcgcttacgc ttatgttgct
     7441 cccttttacc gtatcgctga cgcgtttttc ccacgccttg ctttcgtcaa caagcgttga
     7501 accgacatcg aaaaatatcc actctatcat tctcttatct gaccgtcacc gtaaattata
     7561 tatttaatag aagtcaatgc ctcaagtccc attggacctc ttgcgtgcat tttttgtgta
     7621 cttatgccta tttcagcacc aaatccgaac tcaaaaccgt ccgtaaatct tgttgacgca
     7681 ttaacataaa ccgccgccgc atcaacctca ttcaagaact tttgcgaatg ttcgtaattg
     7741 tttgtaacaa tcgcctcaga atgttttgtg ttgtacttat taatatgctc aatcgcctca
     7801 tcaattccgt caacgacttt taccgccata atataatcat tatattcagt ataataatcc
     7861 tcgtctgtcg cgtcatttac atctgcaaat attgcttttg aattttggtc ggctctgatt
     7921 tcaacattct tttctttcag tgccttgaac ataacaggaa taaatttatc cgctattttt
     7981 ttgtctataa gcaaagtttc ggcggcattg cacacagacg gacgttgtac tttcgcatta
     8041 agcacaattt taaccgccat atcaatatcc gcgtccgcat caacgaaaac gtggcagttg
     8101 cctgcnnnnn nnnnnattaa caaatccaca tatccgttca ttttcataag ctgagttgct
     8161 gtttcacgcg aagtatcttc tattatattt agtgtcccct caggtattcc gacaccgtat
     8221 gccgcgtcct gcataatttt cataacagct ttgtttgaat tgattgcttc acttccgcct
     8281 cttaaaatgc aagcgtttga agttttaagg caaagtaccg ccgcgtcaac cgttacattc
     8341 ggtcttgcct cgtaaattat tgcaataacg cccataggca cacgtttttg accgatagtc
     8401 aatccgtttg gtcttttcca catttttatt acttcaccga ttgggtcggc aagtgctttg
     8461 acctgtctta ctccctccgc aattccgtca atgcgttctt ttgtaagagt aagtctgtca
     8521 atcatagctt gacgtgttcc gtttttcttt gcgttttcta tgtcaatgtt gtttgccgca
     8581 ataatttcat cggcacgttc tacaagtgca tttgcaatag cctcaagtgc attgtccttt
     8641 gtaaccgtac ttactgaaac cattttatat cctgcatctt ttgcaagagt gcatttttcc
     8701 aaaagctcgc tcattattct tcctccttat cgaaattctt cgacacaaac aatgttccga
     8761 caggctttcc ggcaagtata tcgtaaagtg aatcaacatt gtttccgttt gcgattatca
     8821 tatgaatacc ggcatttgtc gctctttcgg cagcctcaag ctttgtcacc ataccgcccg
     8881 tacctctgct tgttcccgca ccgcctgcaa catctctcac ttcgtcaata tcgtatactg
     8941 taggaagtaa ttttgcgttt ttattcttgt gtgggtcatc gtcgtacaag ccgtcaatat
     9001 cggagaaaag tacaagtaaa tccgcgtcaa caaggtcggc aacgactgcc gataaattat
     9061 cattgtcgcc gatttcaatc tcgtcataac ttaccgtatc attttcgttt acaacaggaa
     9121 ttatccccat ttcaataagc gttgtaacag tattttgaat atttctcttt ctgcgcggaa
     9181 ttgcaatatc ctcacgcgtt aaaagtacct gtgcaacggt gttattgtaa cccgaaaaca
     9241 ttttgtcgta aagatacatc agttcgcact gtccgactgc cgcaatggct tgtttgccct
     9301 ttgtatcttt cggcttttca gccaaaccga gtttaccgag tgcaattccc tgcgcacccg
     9361 atgaaacaag aattatctcc ttgccttgat ttcttaaatc agaaagcacc atagacagtc
     9421 tgtctataag ttttaaattc attgttccgt gttcatatgt aagcgtagac gttccgactt
     9481 tgacaacaat cctgtgcgcc atagacacgt cctttaatat atcgttcatt gccatcctcc
     9541 tataaagcat atagttattt ctattatatc gctttatggg ttgtttgtaa agtcattttt
     9601 atcttacctt attcttcaaa attttacttg cattttttta ctctctatga tactatataa
     9661 atatattttt acaaaatttt cattgtatca tataacaata atgtgaattt tattatatat
     9721 tatacaacat tgtgggaggt aatgatgaaa aaacaaataa ttgcggcaac tgcgctgatg
     9781 ttagcgattt cttcaaccgc atatgcgtcc gacaacataa acatattcgt aaacggaaat
     9841 gccttaaaca caggtgcttt cattatgaat gattcgacgt atataccact tagagcggtc
     9901 agtgaggctc tcggttcaaa tgtaaactgg gatggaaata caagaagtgt ttacattgat
     9961 tccgatgaag atacagtcac tgctcaagtg attgaaaacg caagttcaag tgtcgttgca
    10021 atagtaggaa attacaaacc cgaatatatg acagggactg tttcaaatta caacgaactt
    10081 accgctcacg gtacaggtgt agttatcaaa tcaggcggta ttatccttac aaacgcacac
    10141 gttgtaagcg atattgacaa cataacagtc gttttctccg acggcgaaag ctatgccgga
    10201 caagttcagg caatcgacaa agattccgac ttagctttgg taaaggttgg ccgtcttggt
    10261 ttaaaaccaa tatcattcgc gcaatcggac agtatcttcg cagggcagtc cgttgttgca
    10321 atcggtactc ccctttcgct ttcaatgcga aacagtgcat caaaaggtat cgtaagcgga
    10381 cttaacgtca gcactccgag tgcatattat ccgttaattc aaactgacgc cgctatcaat
    10441 gcaggtaatt ccggcggacc gcttcttaat atgaaaggtc agcttatagg tattaattct
    10501 tcaaaatatg caggtgtcgg aattgaggga attaactttt caatcccgct tgacacgatt
    10561 aattatgtac tcaatcaatt tgagcaaaac ggaaaagtca taagaccgga tttaggcgtt
    10621 acacttgaaa attcatggga ggcgcgaatc ggtctgccga caaagaaagg cgtcaccgta
    10681 aaaacatctt cgtcaagttc actttcaaac ggtgacgtta taaactctat caacggcgtt
    10741 gaagtacaca gcattgtgga ctttaataaa gcattgcgtg atacatattc ttcgggaagt
    10801 atcaacgtaa tttatacaag gaacggtgaa caaatttcaa ctgacatcgt tcctaatcta
    10861 aaataataaa attgggggta ttaaaatgaa caaaaaacta atcgctgcaa ttcttgccac
    10921 aacagcagct ctttcagcag caccttttgc gnnnnnnnnn naaagtagaa atcgcattca
    10981 aggtaggtga cagcattctg aaaataaacg gcattgaaac agaagttgaa acaccgtatg
    11041 taacagaaaa caacactaca ttggtaccgt taagagtaat aacagaggct tttggtgcaa
    11101 aagttgactg ggacggtgat actcaaaaaa taactcttac ataccctgcc gtaaatattg
    11161 aacttcaaat cggcaatatc gttgcaactg taaacgacca tacagaaaca cttcttgaat
    11221 cacctgcact ttccgaaatc ggcgttacaa tggttccg
//
LOCUS       DAWACS010000006        11247 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_673_length_11247_cov_2.851322, whole genome shotgun sequence.
ACCESSION   DAWACS010000006 DAWACS010000000
VERSION     DAWACS010000006.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11247)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11247)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11247
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_673_length_11247_cov_2.851322"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6688..6697
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10568..10577
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3602 a         1957 c         2758 g         2910 t
ORIGIN      
        1 agtctgaaaa gagcgacgga cttctttata aaaagaattt gaactgcttc ggcggtagaa
       61 ttcttgttaa tgccgatacg aaattcgtca ctgtcccgga taaaaacagt agatttgaaa
      121 aatactctgt aaatagcatt tcaagcttaa agaatgataa gaattataaa aatctcacag
      181 cttatgcatt aggtaaaaac agtataacag ctgatattat tgttcaggaa ttagccggtg
      241 gaatagatgg tgatattgag ggtgaaacaa atattgcagt agtttcagat gtgatgtatg
      301 gtcttaatcc caaagaagac gaagcagatg ctatggaggt ttatataaac ggcacgactc
      361 agaatatttt tggtgaagga cgcaatacac ttggaaaaac agaaggtgat agtttctatc
      421 ctgttagtgt aggtgatatt gtacgctaca atattgattc ggacggaata gtaacaacac
      481 ttgatccaat ttacattcaa agctatggag aacttgcgat gccagatgat tttgaaaaaa
      541 atatatatgg cactgacaga agatttttgt gctgggcata tgacagggca ggagacttcc
      601 ttggtgtagc agtaaaccct aatcatgaaa ttgtgtctcc ggaaagaggc ggtgttgttt
      661 acttttctgc gagtcaggca aatgtataca tatacgaccg aagcaggaaa aaagtttaca
      721 ctggaacttt agatgatgtt gagacttaca gtactgtaag tgatgaatgc agcaatatat
      781 atgtttcggc atttaagggt gaaattaagg atatagtggt atttcttgac tgatagaaag
      841 gagtatgtaa atgaaaaaga taaaatatct aatcggacta ataactgctg tattagcatt
      901 tacatcatac agcgttatgg cagccgatgc tgttgttaaa gaagcatatt tcaaacagaa
      961 taatgaagtt gtacataccc tttcaaacgg tggtttggaa tgcgttatca atgcttctgg
     1021 tatcaacaat gatgcggtga tgataaatgc aatatatgaa aaaaatacta ataagttggt
     1081 tagtgtccga tatacaaatt caaacagcgg tgaatttaag aatacattag acataaccaa
     1141 tgcgcaaaat acatatgtga caatgtgcat ttgggatagt ttaaatagtg cgaatgctaa
     1201 aacacaggtt tattcaatcg cgtcatcaga tacagctccc gaaaatccgt ggaatgtaaa
     1261 gctcaacaac aagggcgcaa tatcctttaa atgggaagaa tgcgaagata atataaaaac
     1321 tgagggttac aggatataca aaaacggcga aaaaatcggt gatacaaaaa cagcagactt
     1381 taatgacgaa agtgtgaaat ggtataaatc cggaaaagca gagtatcgtc ttactgcata
     1441 tgatgaatac ggcaatgagt cggacggaaa acttctgacc gatgtttcca ttgataattc
     1501 tgatatagca gcagttttgt tttcacaacc taatataaca gaaaaaatgt cattgtatat
     1561 tgctactgga aaggaaacag atgagggctg gtatggttat aatacggctt acatacagga
     1621 tggtgttcct tgcagacgta ttccagctga cggaagagca gtgtttaagt attcaggcga
     1681 ttttaaaaac cgtataggcg atggagtaag agacgctgtt atacgcgtga cctattttga
     1741 caacggtaca gacggattta aggtgtcata tgtgggaaaa ggcgcgcgtt ataccacatt
     1801 taacattgtt aaaacaaata cacagcaatg gattacaaaa gaacaaaaag tgtccgattt
     1861 tactgcggca tttattgacc cgagagaggg tgctaaaact caggacgagg gtgttgattt
     1921 cacaatatgg tcaaaggctg attcagataa aggtgatgtt gtcgatgtag agacatatat
     1981 ttccgaggtt caggtagtgc ttgagggcga tgctccggag cctacaccaa cattggaacc
     2041 gggtcagact ccttctccga cagctgaacc aacaccgaca ccaactccgg ctccgacaat
     2101 aaatcctgcc gatataaaga aggcatctat caacttcctc gcaggacaga cagctgatag
     2161 ctgttcagaa agcgaaaatg atcttattca tatattctgg gcaggaaaga ataatgatga
     2221 acagattaac aattctggtg ataaagctta caattacgga gtaacgcaaa atgacgagga
     2281 atgtcgcgcg attgacgatg ataagcgttt agtgcttata tacagcaatc cgcttaacga
     2341 agcaattgga aatgagccga gaaacgctaa gattcgcgtg agatatctgg atcagggaac
     2401 agataaaatc agaatttcct atatcagtaa aacgaaaaga tattctgacc ttaacataca
     2461 gaaaacagat agcggagact ggaaatggga ggaatttgtc gtaaccgatt tagtgaatga
     2521 aagtgatgac cagggttcaa agggcggcta tcaggatggc agagtggact ctaaggtttc
     2581 gccgatatat cctgacggac cgaaccttac tatatgggca aagggctcag gtgatgccgt
     2641 tggttcaaca gaatatattt ctcaatttga gcttattctt gaagatgaaa ctccggttga
     2701 gacaccacag cctaccgaac agccgaaacc ttccgatatt tcactggaac aggatatgga
     2761 taagtcaacg cttgagaata tagcttttaa atgggatgca gttgagaatg ccgcaggata
     2821 taatgtatac cgcggtgagg aaaaacttac aaatgagcct ataaccaata cggaatatga
     2881 cgacagcagt gaagaacttg tatggtatga ggattataca tataaagtgg aggcggttga
     2941 tattgaaggc acagctcttg gaacaggcac agagaaattt acggtcaata aagataattt
     3001 cgccggaata agactgcttg acggtacaac ctatgacaca acatatacag gtgaaaagat
     3061 gaccgtatac tggggaggca caaatcagca tgttgcaaat acagcatggt ttggctatag
     3121 tgaagcggca gagattggcg gaattaaatg cagaaaaatt ttggctgcac atgaaaaaac
     3181 agacggctca ggtggctgga ttaacggtca gagaatttca ctgagatttg atgatacgct
     3241 tcagaatttg ataggcgcag aaccgagaaa tgcaaaaatt cgtatttgtt attatgataa
     3301 cggtacggac ggaataagag tttgctatat atccaatacg gacagaaaca agacagttaa
     3361 tatccaaaag gaagatacca atacatggaa atggtatgaa cttgatgtaa ccgatttgat
     3421 aaatgcgagt ccagcagaca cgagcgctaa taatggactt caggatgaga agacaaattt
     3481 tgcggtatgg ggcaagacaa aagaggaatc caacgaaaga gatacggata cgtatattgc
     3541 ggaaattcag ttaattttag aataggaggc agcggaacat gatgaagaaa attaaacaag
     3601 cagcggcggc ggttatatcc gctgccatgc ttctatcaat tattccggcg gcgaatgctg
     3661 ccgaggagga aaagccttca tatcccgctc cgaattttaa actgtaccag acggattact
     3721 atacagatga atatgtaata gccggctata cggttcagga tttcggcgct aagggtgacg
     3781 gagtaactga tgataccaag gcgtttcagg atgcaataga cgcggccgct gcggcaggcg
     3841 gctgcaccat ttgggtacct gagggcagat atgctattaa gggaaatctt gttatgaagc
     3901 agggagtttc gattaatgga gagattgata cgcctggcag agataaggct gtaaaaggaa
     3961 ctattttgca ggcatatgcc ggaagaggaa gtatagaagg agatcctttt atcatgatgc
     4021 agccggttac gggtattcat gaccttgcaa tatggtatcc ggagcaggag gctgataata
     4081 ttcagccata tcctcctgct attcagatgg gtgatccgtc tgtttggggt aacgactatg
     4141 ttacggttga caatattgaa tttgtcaacg catatcttgg ctataaaaac aaccctaata
     4201 tgggcggttg tcccattgtt cgtaatatat acggcacacc gctgaaaagc ggaattcgca
     4261 tagattctct tgccgatgtc ggcagaatag agaactgcga tttttcaccg ctgtattggg
     4321 aaaactcggg acttccggga gctcccagcg ataaagattc gcacagaagc tacatatatg
     4381 aaaacggtat agcatatgag atgagccgta atgactggtc atacggacgc tggcttacgg
     4441 cggaccgtta cggtgttggc ttttatgcgc taaattcctt ctgcgaagga gcatggaatg
     4501 gcgaaagccg aatatcaaat atgtacccta acggtgaaaa ttacggtatg aagtttagca
     4561 attgccatat aggtgttgcg attgaggata cctcaaattc aggtatggtt atcacgcata
     4621 cggagattga taactgtgaa aacggttttg tggcaagaaa tacaatggca agtaaaatat
     4681 cgcttatagg ctggactata aacgcaacgg ataacgccat attaaacgag ggtacaggtc
     4741 aggttatgat gcaggagatg gatatagaga gcggtaaggt ttctctttca gcaggctatg
     4801 caagcatgct taattgtaag tttaacaatg cagctcctca gataacgctt ggcaagtaca
     4861 gcaatgcttc aatacttgga agcacattta aggaaactaa aacaatagaa aacaattcag
     4921 ttctaacagt tacagttgat gatactcctg ttgaaatgga acccactcct gaatttcctc
     4981 aggtaaatgt tctggaggtt aagccgccga gagctgttat gtataatgct aaagatttcg
     5041 gcgtaaaaat agacggtgta accgatgatg ctccggcaat ccagaaagcc cttgacaaag
     5101 cggcggctga cggcggtggt atcgtatttt taccgcccgg attctatggc attaagtctg
     5161 aattaacagt gccgtcgaat gtcgagttca gaggcgcgct tgatacaggt actatgccga
     5221 gaaagaacgg ctccacgctc gaaatatatc acggcaaggg tcaggaaggc ggagcgacaa
     5281 ttcatcttca ggagaaatca actatacgcg gtatctgtgt agattatccg gaacaggatt
     5341 ataatgaccc tattaaattc ccttacactg tgcagggtat gggtgcagat gtttcaatta
     5401 ttaatatggc aatcagaaac gcatatgatt ttgctgattt gttcacatac agatgcgaca
     5461 gagcatatat ggaatatctc tgcggttcat gctggcatat tggcgtgcgt gtaggcgccg
     5521 gcagcgagga ttgcctcata tctaatactc agatgaatta taacacagtt actacaggat
     5581 atgagagcaa gcatggatcg tgggaaggca gtggaactcc ggtcaagaat aacaacgacc
     5641 cggtgattaa ggaaaaatat gacgctgctg tttcggcgat tgatggatat atgaaggata
     5701 attatcagcc cttcattctt ggtgacacaa agaatatact gttttacaac gattttatat
     5761 tcccggcagg cgtgggattg aggcttatga gcgaaaatgg cacgggtcct tcaggactat
     5821 cgcttggata tggctcagac aattccagaa ctgcattgtt ggttgaggat ataggagagg
     5881 gaggcttttc gtttataaat actcagcttg ttggtattaa cagccatgat aagatttcgg
     5941 gcggatttga aacggcaccg tcattcaaag gaaaggttac tctgcacggt atcgcaatat
     6001 ggggaagtct tggtcacttc gttgagtgca acggcggaac gatagagctt caatccggaa
     6061 atttcgcatc ctcgctcgac aggtgcttta ttcttaacaa cggtcagctt atagtgagaa
     6121 atgcaaagat aaacacaatc aaggaacgtc taatggacgg aaatacggga aacgcatcgt
     6181 ttgttggcgt gaactatctt cacaatgctt ctatgcagga aattgttccg ggttcgtata
     6241 aggaatggac aggaaaccat gatttgcagg gagtagaatt tagaagggtt aaaaatccgg
     6301 ctgttaccgt tccgcttgct gatttatcta tttcggtgag agcgggagga acgaagctta
     6361 atatggaaaa tcaggtgcct tttatagagg gcagcacgct ttatgttcca atggagaaaa
     6421 ttcttgagca gtttggatac tcatatgaaa ttgacaatgg ctcaaaaaca ctttctgtct
     6481 cgagaagtga taagtcaatt atggtaactg cggacagcat atatgccatt gcggacggca
     6541 gaaaagtaac gctgtctttg cctgccaagc tgatgaacgg gagtatgatg gttccgtcgt
     6601 catttattac ggatgccata ggctcacaga tttcatggag tccaaagaat agagaattga
     6661 cggttaatat accaaatctc cccgacgnnn nnnnnnnacg tagataagaa ttcaacaagt
     6721 ccgattgtta agctgaatac atattcaatt tcagcaactg cacaggaaac tgcgaatgtt
     6781 gcctcatatg ctgcagacgg caaaatggat acaagatggt cgtcaatcgg tgcaaacaac
     6841 attatgattg atttgggttc aaagcatccg ataacctcaa taaagcttgc attcttctcc
     6901 ggtaatccga gagtatatcc gtttactata gaggttagtg aggacggcaa gacatggaca
     6961 caggtgcttg acacaaagaa cagcggaaca acagatcaat tagaagaata tgtcttggaa
     7021 acacccgcat acggaaggta tgtatgcgct aagtttaacg gcaataatgt aaaccaatac
     7081 agcagtattt gggaatttga gctttatgga agataaggag gacggcatga agaaatttaa
     7141 aagaattatt tcagtgatta ttgccgctgc tctcttaggt gcaaatgttg ctgcggcaga
     7201 ggtgacgaag attaatgaca aggcagaagt gctgtatcgt cttgggctga taaagggcgt
     7261 aagcgacacg ggctttgacc cgggattagg gatgatttgt acgcgcgagc aggcgattgt
     7321 tgcagttctg cgtgtgacgg gaaatttgga taatgtattt aaagaaaatt ataactgctc
     7381 ttttgctgat gtttcagaat gggcaaagcc gtatgttgca tacggtgaaa gtataggatt
     7441 gtgttatgga attgaggatg gttatttcgg cgcagctgag aatgtaacct taagacaatt
     7501 atgcgcaatg tatttgagaa tgcttggata taaaggaaac gcatcgtctg atatttatga
     7561 acatgcgctt gagacggcaa agccgttggg aatgtgttct gctgacactg atatgaacgg
     7621 aagtcgttca gaccttatag atatatcata taattcgctg tatgtaagcg tttacggcgg
     7681 tacaaccgcg cttgtgagat atttagcgga caatggaatt atgtcggtat ctgatatagc
     7741 cggatgcggt gacgatggac ttataagcgc gtattattca agtgaaccgg cagccggtgt
     7801 tgtttccgat gagcttcgcg gcgatttgga gcatgccata agttataact tcgctgaagg
     7861 cagcggaaca ggtatgtcaa tagcgacaga cagtgagaaa acggaatatg aaggagtaac
     7921 gtgcgaaaaa ataaccgtag gaaagaattt gtcatttaag cttgacgacg gatatattac
     7981 ttctgatgat aagtacttta cattggttgt aaaatatttt gatagcggcg ttgacagaat
     8041 acgtattgca tataatgtcg caaacatgcc gtattgcagc ggatatattg ccaagacaaa
     8101 tacaaatgtc tggaaggaag caaggttagt tataaagaat gcgcaatttt ctcattcaca
     8161 gagcgatggc gctgatataa gcatatttgc ggaaaacgag agcggcgcag gtgtagacga
     8221 gtatatatca gaggttatgc ttgtcaagca gtcgcctgat gaacaatatg taaatccgct
     8281 tgaggttgaa cctattgtta cgacggctgt cataagcttt gacggaatgg gacagaacgt
     8341 atgcctgacg cagtccacaa caggaacagc cacagcctat tattacacaa agcaaactga
     8401 acagggtggc gagggctgta tttcaatagg tgtcggaaag agatgtatat tgaatttaga
     8461 tgatgatttt atggatccgg acgatcataa ttgtcagata aatataacat atttcgacca
     8521 tggaacagat agaattcgtt atgcatactc aacagaggaa aaccgctata aggatgtata
     8581 tattcagaag accggcacag atacatggat tacgcatact cttaatgtaa ctgacgcttc
     8641 gtttatgaac cgtcaggacg gaggtattga tttcagtatt tggggacttt ccgccgagaa
     8701 ttcaaaaaca ggcgatgaaa atgaatatat aagcagagtt gaaattatta agcagtgaga
     8761 ggataaataa aactatgaat attgaaaaaa tcaaaaaagc gcttgaatac tgcatagaca
     8821 gggaaagaga aaatattgat attttcggtt ctctttatcc gtgtcctcag agccgcgact
     8881 acttgtacga cggtcagaca aattatgaat ggactaacag cttttatacg ggtatgcttt
     8941 ggcttgcgta tgaaatgaca aaagaggaat gctttatgaa aaaagcgcag gagcaatccg
     9001 ccgatttcag agacagactt gataacaggc ttgatactct taatactcac gatataggct
     9061 tcctttacag tctttcatgt gttgccgact acaagataac aggcagtgag aaggcaaaga
     9121 aaactgcact tgaagccgca gagttgcttg caaaacgctt cagcgataag ggaggcttta
     9181 tacaagcatg gggtaatctt aacgacccgg caagccataa gcttattatc gactgcatgc
     9241 ttaatattcc gcttttgttc tgggcggctg agactacagg tgaggaaaaa tacagaaata
     9301 tggcggtgtc acatctgaaa gcaactgttt caaatgcgat acgcgacgac tttacaaccc
     9361 accacacctt ttacttcgac aaggaaacgg gcagaccgtt gaggggtgaa acacatcagg
     9421 gctacagcga tgattcctgt tgggcaagag gacagtcgtg gggaatatac ggcacggcgt
     9481 taggctactc atatacgaaa gatgaaagta taattccgat attcaacgga cttgtggatt
     9541 gtttcctaag caaattgccg gaggataaag ttccctactg ggatatgatt tttacaaacg
     9601 gcgatgagcc gcgagatact tctgcggcat caataacatt atgcggcata ctcgaaatga
     9661 ataagcatgt gccaaatgaa cgatatatgc aggcggctga ggagatactc aattcactta
     9721 ttgacaaata tatgactgat aaaataacct ctacggggct tttgactgac ggtatgtatg
     9781 cccgaatgag aggtgacaga cctgagtgta acatatgggg cgactattac tttatggaag
     9841 cacttgtcag aatagtctca ggcggcgaat ggaaaaagta ttggtaaaat taaatattta
     9901 agcagtatat tcattataac gatgaacatg ctatttaagc gatattcata tcgtatatat
     9961 tacaggagga cgtagctaag cggcgctgtt atcggcgctg cacggctgcg tcttttaaca
    10021 ttaaataaat tttatgcgga gtaatgtctg cgctttgcat aataacaaat atttgcagac
    10081 ggaaaggctt ggaaaagatt atgaaaaaaa ttaaaaagaa aatcagcctg ctgctgtcgg
    10141 caggtctgac aatgtcgtgt atggctgccg taccgaatgt gagccttgca gcggatgagc
    10201 agattattcc gtttggcgcg gtgtgctcgt ttgataagtt tacattatcc tataaggacg
    10261 cgcagacggc tgatgtgatg attgcgtttt cggaggacgg aacaaattac ggcgagtatc
    10321 agacggtaag cttttcaaat gacaccgatg aaacaaagta tcttgaaagc acagctaaag
    10381 caaaatgtgc caagctcagg gtaggtagcg agggactgcc gaagattaag cttgaacaga
    10441 ttgtacgata ttcatcgtca aaagccaatg tcggacttgg ctcaagaggt atcgacggaa
    10501 atacaaaacc gatgtggagt gctgccaatg acaattttac tcagttcttt gacggcacat
    10561 acaatgcnnn nnnnnnnata caatgcggac ggctctatag caaatctcgg aaagtgcaat
    10621 cttacaggtc tttatcatga tgcgataaaa gaaggtaaga caacggataa gtcattggca
    10681 atggtagagc ttccttttgt atttgatgaa gctttggaat ttgcgtatgc gattgtctat
    10741 atggacgctt caaatgcggc ggatttctat gtgcgtattc ctgataaaaa cgcgaaaaac
    10801 agacacgagc ttaccaaagc gaacgatttt ggcgagccaa aaacaataga ttcagaaacc
    10861 ggcaataaga gcgttttcgt tataaaagct ccgctttgca atgagggtga aaaaacgaac
    10921 agcgttgatt tttattggca caacttccac aattatacgg gaacgccgga aaccgctatg
    10981 gtgaatatgc ctgagatagc gttctactat tacagtccgg tgtttgagtc tgagtacata
    11041 cttcatgacg gagcggagta tacttttgac actccgaaga ccttttcaag gattgtcgga
    11101 gagcttcctg cggatatgtg gtacgttggg ataaaggctg acggcggcga agccgatttc
    11161 agagtgaatg aaggcgccac gggttatttt gcggataatc ttatggagat aaagaaaata
    11221 atcgtgcggg gatataacgc agaaaat
//
LOCUS       DAWACS010000007        11073 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_688_length_11073_cov_2.408332, whole genome shotgun sequence.
ACCESSION   DAWACS010000007 DAWACS010000000
VERSION     DAWACS010000007.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11073)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11073)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11073
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_688_length_11073_cov_2.408332"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    316..325
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8428..8490
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3356 a         1801 c         2472 g         3371 t
ORIGIN      
        1 cttttgttta cgttttgagc cgtcataata taaataacgg aaaaactcga taaaacaagt
       61 gccgcaatta tgagcgtgtt tattataaga agtttatttc taagtttttt aaacataccc
      121 aagtccccct attcgcttaa agtgtagccg acacctctta cggtgttaat ggacaccttt
      181 gaatgtacga acgcaagttt tttgcggaga aatgatatat atacttcaac gtgattggat
      241 tcagcctcag aatcaaaacc ccataatttt tctataatct gttcctttga acaaaccatt
      301 tctttgtgta acattnnnnn nnnnnatgtt caagcagttc gctttctttt aatgtaagag
      361 ttatttccga cgtacctgcc gtcagcttta atgaagaagt gttaagagtt atatcgccgt
      421 atgagatagt atttgtgttt gaaactattt caccgcgtct gcggcctaat gcccttattc
      481 ttgcaagcag ttcttcggtg ttaaacggct ttgcgaggta atcgtctgca ccgctgtcaa
      541 gtccgagaac tttgtcggaa atttcatttt ttgcggtaag aagtataaca ggtgtgtcaa
      601 aaccctccga acgcaaggtt tttaaaacgg ttataccgtc cgttttgggt aacgttatat
      661 caaggacaat gacgtcatat ataccgctta atgcgtcgtc aagtcctgtt tcaccgtcgt
      721 ttgcaacgtc aacggtgtaa ttgtttttct ttaatatatg tgacaaagcc tccgataaat
      781 gaagctcgtc ctccacaaca agtattcgca ttcgctcacc ctcttttatc attttatatc
      841 tatattatag catagttttc ttaaatgtac cttaatttta aaagtttttt tacgtattat
      901 atagtagaaa tttcaccgga gaaaagtttc ctgaaattaa ttttacattt taaggtttat
      961 ttaaggatta taccgtacaa tgaaatcaaa gaaaggataa aagaggagat gaggacttat
     1021 ggcaatagag atttttaatc gttacgagca taaatataag ctcgacacga aaacttttaa
     1081 taaggttttg gagattatgg atgagcatat ggaacttgat tcgcattgcg gcgaacattc
     1141 gctttatacc atagcaaata tttattacga cacgaaagac aattctctta taagagagtc
     1201 gctttcaaaa ccggcatata aggaaaagtt aagacttcgt tcttacggtg taccggatat
     1261 ggattcaaag gtgtttcttg aaataaaaaa gaaatacaga ggtcttgtga ataaacgccg
     1321 tacaacattg gaacttggag aggcatatga ttttattgaa acaggagtga aaccggcatt
     1381 acaggattat atgaatggtc aggttttgaa tgaacttgaa tattgcctta atttgtacga
     1441 ccttgagccg aaagtctata tcgcatatga caggttggct tactttgaaa aaggcaatga
     1501 cgatttgaga atatcgtttg atacaaacat tcgtacaaga agaagtgatt tattgctgga
     1561 gtcgggtgac cacggaaaaa gacttttaga cagtgacgtg tggcttatgg aaataaaaac
     1621 aagcttggca aaaccgctgt ggctttgcga aatgctttcg gaatttgaaa ttcgttcatc
     1681 aagtttttca aaatacggta cggagtacaa aaatatgatg gcaagacaaa aggaaatgcc
     1741 gaaacaaaaa atatttgtat tcggaaatca aaaacaagca gtataatatg ggaggataaa
     1801 agagaatgga taatttaata aattcaataa catcaacatc gtattcaggc ataacgctaa
     1861 ccttgtctat ggcaatagtc gcaatggcag cggcacttat attcggactt gttataagtc
     1921 ttacatatat gggtacacat aaaaataaat atcagcagag ttttgcgatt acgcttacaa
     1981 tgctgccgat aattttgacg gttataattt tgtttgtggg aagtaacgtg gcgagagcat
     2041 tttctcttgc gggaacattg tcaattattc gtttccgttc ggcacccggc gatccggagg
     2101 atataggata tatttttttt gatattgcgg caggtcttgc gtgcggtgta ggtctttacg
     2161 gatacggagc attatttgtt ttgttgcttt gcctgtttat gattgtgatt tcaaaaatca
     2221 aattcggtaa gcctaataca acggctaaaa atttgaaaat taccattccc gaaaatcttg
     2281 actatgaggg tatatttgat gaaataatca agaaatactc aaaagattat acattacaaa
     2341 aggtcagaac aacagatttg ggttcgctgt atgaacttac atattcggtt aatatgaata
     2401 aagatgccga tgaaaagaag tttattgacg aattaagatg cagaaacggt aatttgaaca
     2461 ttgtattgtc acttgccgca acagagcttt acggaaaata aaggtttaat aatatccgac
     2521 tgttttaaca agaaattaat ctccccccta tacagttaag acagaggata tattaaaacg
     2581 gataataggg ttggaaaaaa gtttaaattt ttctaaaaaa gacttgcaaa gtgttttgcg
     2641 gtatgctata atatatttgt agaaagaaat tacattctac ccttttatcc tatacatagc
     2701 aaaatgaaaa gagcacttat ttaggtgctt ttttcatttt tttttgataa atttttcttt
     2761 atagggtaag ctaaaataaa ggagagtggc ttaactatgg caggaataat tttcagtata
     2821 atagcgggtg cgtcaatgag tatacagggt gttatgaata cacgtctttc ggataaaata
     2881 ggaatatacg aatcaaacgc attcgttcag gggacggcat ttttattgtc gcttattgcg
     2941 gtgtggataa tgggcaaagg cagttttaaa ggtatagcgg acgtgaataa attctatctt
     3001 ttgggcggtg tactggggat aattataact gtaacggtta tgctcggtat aggcaaatta
     3061 agtcctacgg tggcaatttc aattatattg atttcacagc ttttcgttgc ggcgttgatt
     3121 gacgcgttcg gtcttatggg cagcgaaaag gttgcgttta cttggaataa atacatcgga
     3181 atggcactta tgataggcgg tgtgctgttg tttaaatgga aatcataaaa tatcacaact
     3241 aattctacgt taatattatg taaaaaaaaa ctgtttttta ttgcaaacgt aatacaaaat
     3301 atgatataat tttttttgca tttatgcgaa ataaaattta tttaaaattt atcgggaggt
     3361 agagaattga acaaaaatat tattagtctt gaaaacattg ccgtttcctt tgacggtgag
     3421 aagattttaa acgacatcaa tcttgacatt aaggacaaag agtttgtaac gtttctcggc
     3481 ccatcgggct gcggaaagac aaccacactt agaattatag gtgggttttt aaagcctgac
     3541 agcggtattg tcaggttcga cggtaaggag attaataata tacctcctta taaacgtaat
     3601 gttaatacgg tgtttcagag atacgcgctt tttcctcatt tgaatgtgta tgataatatt
     3661 gcattcggat tgaagataaa gaaattttca gataaggaaa ttcaaaagcg tgtcggagca
     3721 atgctggagc ttgtaaacct taaaggtttt gaaaaacgct caatcgacag actttccggc
     3781 ggtcagcagc agagagttgc cattgcaagg gcacttgtca atgagccgaa ggtgcttctt
     3841 cttgacgagc ctttgggtgc acttgatttg aagcttagaa aagatatgca gattgaatta
     3901 aagcgtattc agcaaagcct tgaaattacg tttatatatg taacacacga ccaggaggag
     3961 gctcttacaa tgagtgatac ggtcgtggta atgaataacg gtgatattca gcaaatcggt
     4021 tctccggttg atatatataa tgagcctaaa aacgcattcg ttgccgactt tatcggtgaa
     4081 agcaatatcc taaacggtat gatggttaag gatttgcttg ttgagataaa cggtcacaga
     4141 tttgaatgtg ttgatacggg attcggtgaa aataaacctg ttgacgttgt tatacgtcct
     4201 gaggatattc agcttgttaa gcctgacgat tcacatattt cgggtgttgt gcagtctgtc
     4261 acgtttaagg gtgtgcatta tgaaatgctt atcgaggcat atgaccacga ttggcttgta
     4321 cattcgacaa aggcggcaga agtgggaagt acggtcggta taaagttcga tccgtatgac
     4381 atacatatta tgcacaagat gaacgattaa ggaggtagat atgatgaaaa aacttgtttc
     4441 cgctccttat cttttgtgga tggcgatatt tattattgtt ccgcttataa tggtggcata
     4501 ctttgcttta accgatacaa acggagcatt tacgcttgac aatcttgcgg cggtgtcgca
     4561 gtattcaaat atatttattc gttcaatatg gcttgcggcg gtcgcgacgg tgatatgttt
     4621 tatcatagcg tatccgcttg cactgatact tgccaatacg gataaaagct atcaaagtac
     4681 ggtgcttatg atagtaatgc tcccaatgtg gatgaacttt cttttgagaa cgtacgcatg
     4741 gatgacactt ttgggcaata acggacttat taacagcttt tttgggcttt tcggacttgg
     4801 cccgtttaaa atgctgaata catcgggtgc ggttgtgctt ggtatggttt ataactattt
     4861 gccgtatatg atccttccac tttatactgt aatggaaaag atagataaaa gcgttatcga
     4921 ggcggctcac gatttgggct gtaacaatgt aaagactatg ttcaaggttg ttgtaccgct
     4981 gagtatgccc ggaataatgt cgggtatcac aatggtgttt gtgccggcaa tcagtacatt
     5041 tatcatttca agaatgctcg gcggcggttc taacctgctt atcggtgacc ttatagaaat
     5101 gcagtttctc ggaaattcgt ataatccgaa cttaggtgcg gcaatttcgc ttgtgcttat
     5161 ggttatcatt ctgctgatta tgacaatact taatcagttt gatgatgatg aagataaggt
     5221 gcttatgtaa gtttataaga aaggacgtgg gatttaattg aaaaaattat caaaggtcta
     5281 tatggctctt gttttattgt tcctgtatat accgattttt gtgcttatcg tgttttcgtt
     5341 taacgtaaca aaaagccgtt cggtgttttc gggatttaca tttgattggt acataaagct
     5401 gtttcataac ggacttataa tgaaatcgct tttaaataca gtaatagttg cgacagttgc
     5461 gtcaatcgcc gcgacaattc tcggtactgc ggcggcaatc ggtataaaca atatgaaaaa
     5521 actgccgcga acgattgtta tgcagggtac gaacattcct attatcaacc ccgaaatcgt
     5581 tacgggtgtt tcgcttatgc ttttgttcgt gttctttgcg gcaagaatga attttgaatt
     5641 tggctttgtg acgcttatca tagcacatat cacgtttgac gtgccgtatg taattctgaa
     5701 cataatgccg aaattcagac agatgaatcc gcatctttac gaggcggcac aggatttggg
     5761 gtgcagtccc gtttcggcat ttttcaaggt tgttttgcct gaaatattgc cgggtgtaat
     5821 ttcgggattt ttgatgtcgt tcacattttc gcttgatgat tttgttatca gttactttac
     5881 aagcggtgca acgtcacaaa cattgccgat tacgatttat tcaatgacaa gacgtaaggt
     5941 aagccctgaa ataaacgcac tttcaacaat aatttttgta atcgtggtta tagtgcttgt
     6001 tgttaagaat gtaattgagc gtagaaatgc ggtaaagcga ggtgttaaat gatgaaaaaa
     6061 tttatacttg ctttaatttc aatgtcgctt ttgcttgcaa gcggttgtgc gcatattgag
     6121 gaatacgata ctgaggaaac ggcagatacg gaagaaacaa cggaagtaca gactgtaacg
     6181 gttgataatc ccgaatacta tacgcggttt aagaatgacg gcatatcaat aaacgtgtat
     6241 aactggggtg aatatattcc tgacggaagt gaggagggcg tacttaactt aaacgccgaa
     6301 tttacaaagc ttacgggcat aacggtaaat tacagtacat atgctacgaa cgaggagctt
     6361 tacgcaaagt tgaagggcgg cggctcgact tatgatatta tcattccttc agattatatg
     6421 atttcaagaa tgataaaaga gaatatgctt gaaccgcttg ataagtcgaa tattccgaat
     6481 tttgcaaata taatggataa gtttaagagc tcggaatatg atcccagctc aaagtattcc
     6541 gtgccgtata catggggaac ggtcggaatt atctatgata cgactgttgt agatgaggag
     6601 gatattggct gggatattct ttgggacgaa gattattcgg gcagaatatt gatgttcgat
     6661 aatccgcgtg acgcatttgc gattgcggaa aatatgcttg gatattcgct taatactgaa
     6721 aacagcgaag aacttgaaaa agcggcggaa aagctgaaag aacagaaaaa agttgttcag
     6781 gcgtatgtaa tggacgaaat atttgataag atgggtgcag gtgaggcaat aatagcaccg
     6841 tattacgcag gtgacgctgt aacgcttatg gacgaatatg aggaccttgg ttttgtaatt
     6901 cccgacagcg gtacaaactt gtttatagat gcggtttgta ttcctaaggg ctgcagaaat
     6961 aaagaggcgg cggaaatgta tattaatttc ttgaatgagc ctgacgttgc atatgcgatt
     7021 gcagatttta tcggatattc aactccaaac caaaaagcat atgaaatgct tgatgatgaa
     7081 gtgaaaaatg acggtatatc ttatcttgat gatgattata tcgaggaaaa aacgactgtt
     7141 ttctgtaatc tttcagatga ggcaaatcag aaaatgcaga cgctttggac tgatatgaag
     7201 tcgtcagaag aacaaacacc gaataaagtg atagttccgg catttatgtc agtgtgcgtc
     7261 attgcgtcat tgattatatt aataagaaga tatattaaaa agaagaaaga tatgttttaa
     7321 taaacggcga ccgttggtcg ccgctacgag gtagtagtgg cggtcatcga ccgccctttt
     7381 gacttatata tattaatatg ctataattat atataagttt aaattaaggg gataaaaaaa
     7441 tgatacttgc aatagacatt ggaaatacaa acatagtggt aggatgttgt aaggacaaca
     7501 aagtttcgtt tgtggaacga atttctacaa atcacactgc aacggagctt gaatatgctg
     7561 tttcgataaa gacgattttg gagctttaca acattgattt ttctgaaatt gacggtgcga
     7621 taatatcatc ggttgttcct actgtcacaa tcactatgaa aagagcgata aagaaaatta
     7681 caggctgtga ggcaatgatt atcggaccgg gtataaagac gggtttaagc attgttatag
     7741 acaatcccgc gcagctcggc agtgacttgg ttgttgacgc ggttgcggga atacatgaat
     7801 acggtgcgcc gattataata tttgacctcg gcaccgcaac gactatttcg gtagtcgata
     7861 agaataacaa ctacatcggc ggtgttataa tgccgggaat gggtatttcg cttaacgcaa
     7921 tggtaagcgg tacgtcgcaa cttccgagaa ttagccttga agaaccgaaa aagattatcg
     7981 gctcaaatac cgttgactgt atgaaaagcg gtatattgta cggtactgcg tcaagtatgg
     8041 acggaatgat tgagcgtatc aaggaagaaa tcggtgaggc gaaagttgtc gcaacaggcg
     8101 gacttgcaag tacggttgtt ccgcattgta aaaatgacat aattcttgat gacgaacttt
     8161 tgcttaaagg acttatgata atatataata aaaacattta atttcaataa attcccactt
     8221 gtagaatagc agaatatgca caaaatacta acatagttta atggaaagcg gggaagagaa
     8281 tttgaagaat aataaagttt ttgaagttct gcaacgcgtc ggcaggtcgt ttatgctgcc
     8341 aattgcaatt ttgcctgttg caggactttt tctggggata ggaagttctt tcacaaatga
     8401 aacaatgctt aaagcatacg gtctttannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcataatggg accgggaacg ataattcacg
     8521 gcattttaac ggttatgagt gccgccggaa gtatcgtttt tgataatttg ccgttgcttt
     8581 ttgcaatcgg tgttgcaatc ggtatggcga aacgtgaaaa ggaagttgcc gcacttgcga
     8641 gtgttattgc gttttttatt atgcacactg ccataagtgc gttgatttcc gtaaattatg
     8701 ccggcggcga tatgagtatg gacgcaatgg aacatgctgt tgtgaatttg gagcaaaatc
     8761 tcggtctgag cggtgcaacg tcaatggtac ttggtatata tacgcttaat atgggcgtgt
     8821 ttggcggtat tatagtcgga cttggtacgg cggcactgca caacagattt tacaaaatac
     8881 aacttccgca agtgctgtca tttttcggcg gtacaagatt tgtgccgata atatcggcgg
     8941 ttgtgttttt gcttgtcgga atacttatgt attttatttg gccgtatatt caaaaagtta
     9001 tttcaatgct tggaaatctc gtacagaata cgggctatat cggtacactg atttacggaa
     9061 taatcgaacg tgcgttaata ccgttcggcc ttcatcatgt attctatatg ccgttttggc
     9121 agacaaatgt cggaggctca atggagattg caggtcagac aataaacggt gcacaaaata
     9181 tattctttgc acagcttgcc gcccctaaca caacgaaatt ttccgttgac gcaacgcgtt
     9241 ttatggcagg taaattcccg tttatgatgt tcggtttgcc gggtgcggca cttgcaatgt
     9301 acagatgtgc cagaaacgaa aagaaaaaag ttgtcggagg acttttgctt tcggcggcat
     9361 tgacggcatt tttgacaggc ataacagagc cgcttgaatt tacattcttg ttcgttgcac
     9421 cgattttgta tgttgtgcat tgtgtgcttg ccggtatctc gtttatgctt atgcatatat
     9481 tcggtgtcgg agtcggtatg acgttttctg gcggacttat agatatgacg cttttcggta
     9541 taatgcaagg taatgcaaaa actcattgga tttatatcgt tcttgtcggt attgtgtatt
     9601 tcttcgttta ttggggtgta ttcacgttcc ttataaagaa atttaacttt aaaactccgg
     9661 gtcgtgaggc ggataatgag gaaacaaagc tttatacgcg ttcagatgtg aatgcgaaaa
     9721 atggcggaaa gacagatatg acatctgttt tgattttaaa aggacttgga ggtaaagaaa
     9781 atatcgccga tattgactgt tgtgcaacaa gacttcgtat tacggtgcat aacagtgacg
     9841 cggtcagcga ggatatttta aaacaaagcg gtgcggcagg tgtaattaaa aagggtaacg
     9901 gtatacaggt tatttacggt ccgcgagtaa cggttataaa gtcgcatttg gaggacttta
     9961 tggaaagtaa ggaaagcgtc gatttaagcg gttatggtgt ggcggataat gaaatacaaa
    10021 ctgaaaaaga aactgcaccg aaagcagacg gaacggaaat attcctttca tctccgataa
    10081 gaggaaaagc agtaccgctt gaaaaggtgg atgatgaggt attctctgcc ggaatactcg
    10141 gacaaggtat tgcgattgag ccgagcgagg gcaaggtttt cgcaccggtt gacggtgtgg
    10201 ttgaaaacat tccgaaaagt aagcacgcta tcgcaataac tgcggataat gatgcaaata
    10261 ttttaattca tgtcggtctt gatacagttg aacttgatgg taacggattt gacgtaaaag
    10321 ttgcaaacgg tgcaaaaatc aaaaaaggcg acttgcttat gacgtttaat ttaaacggaa
    10381 taaagaaaca gggctataaa atgataacgc ctatggtagt ttgtaacgca gatgaatttg
    10441 cggaatttaa aaccgttgcc gacggtgatg tgaatgtcgg tgatgatgtg ataagaatag
    10501 tcagataaca aaaaggacgg ttttaaaacc gtcctttttg aatatcaaat attttgcacg
    10561 ttccgaacaa ttatcggatt ctgaattagt ctatcttaac tgtccaaccg aactcgtctt
    10621 ttattttgcc ccattgaata cctgtaagag tatcataaag catatgagta acttcgccga
    10681 tttcgccgtt gtttacaggc attacaaggt caccatactt aagttcaccg ataggtgaaa
    10741 taacagcggc agtacctgta ccccaagctt cttcaagctt acctgctttg aatgcctcaa
    10801 caagttcatc gattgaaaga cgacgttctg aaactttgta accctttgac tgaagaagtt
    10861 caataattga cattcttgta ataccgccaa gaattgagcc tacaagagca ggagtaataa
    10921 cttcgccgtc aattttgaag aatacgttca ttgaaccaac ttcttcaacg tactttcttt
    10981 caacaccgtc aagccataga gtttgaacat aaccttgttc agcggcctct tcctgacctt
    11041 ttagagaaat ggcatagtta gcaccggctt ttg
//
LOCUS       DAWACS010000008         9926 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_783_length_9926_cov_2.348293, whole genome shotgun sequence.
ACCESSION   DAWACS010000008 DAWACS010000000
VERSION     DAWACS010000008.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9926)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9926)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9926
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_783_length_9926_cov_2.348293"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6493..6502
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8025..8034
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3220 a         1607 c         2371 g         2708 t
ORIGIN      
        1 gtggtcggtt gcaggacttt tgacagcact tgtaatagca gcattgcttg gattcggacc
       61 gctttcgtca tacgttgacg aaacaggtaa aaccgtaaca ccgtttcttg ataacatcat
      121 attgataatt acattcatat tctttgtccc gggaatgttt tacggacatg ccgtgggtaa
      181 gttcagaaaa ctcagcgata tggtcggagc tatgtccaag caaatcggta caatgggata
      241 tgcgattgtt ttgacgttct tcagctataa cttcctgtca cttttgactt atacaaattt
      301 gggaacgtat ataacgtata tcggagcaat gggcttgcag acggtcggtg cgtcaaattc
      361 accgatattg cttataatcg gctttatatt gataacggga tttattaatc tgtttgtcgg
      421 cggattgagt tcaaaatgga tgttgctcgg accgattttt gtgccgatgc tataccgtgt
      481 aaacagctca atgacgccgg acgttgtcgc ggctgcatac agaattgccg attcgtcaac
      541 gaatatcata acaccgctta tgacgtatgc gggagtggtg cttatgtata tgcgaaagta
      601 taaaccggaa tttacggcag gtaacttaat atcaatgatg attccgtatt cggttatatt
      661 tatggtatca tggacattgc tgttaatggc atttattctg ttcaaaattc cgttgggatt
      721 ttaaaaataa aaagcgcgaa tctgtttaga tttgcgcttt tttgatttga aaaggttatg
      781 tttgaataaa tgaataaaaa ataacataat atataggaag gtttgaaatt aagacaatta
      841 aacaaaaagt gaaaatatac ttattgttct ttgtgcaaag tgcaacaaag aaaaggataa
      901 atttgtgtat aagtgagaaa ataaaagaat aaacgacaaa taacgataaa attgtttaaa
      961 aatatacgtt tgtcggaaaa ctaagggttg aaaataataa gtattatggt aaaatagata
     1021 tagtatcaaa aagagaaaga ggggaaatat gtgtcaaaga ctttacttgt cgaaataaga
     1081 aatattgcaa taggtgtaat catactcgga gcggtgcaag tacttgttac tttgccgaca
     1141 aagttttttg gtttatctgc aatattcgga acacttctcg gatgtgcggt ggcaatttta
     1201 aactttgcac ttatgggaat tatccttgaa agatgtgtgt caaagcaaaa aggtgcgtcc
     1261 ggacttatgg gagtcggtta tataggtagg ctggcactta ttgcacttgc ggtaatttgg
     1321 gcaacaaagg ttgattatct aaactatgta tgcgttataa ttccgcttat attcccgcag
     1381 atttctatac tcatactaaa tcttacgaga aaaaaggaaa gggaagtgtc agaagatgaa
     1441 cggacctaag gttttatttg agataccgct tttcggtgga attaaagtaa ccgaaagtat
     1501 agtcaatatg tggattataa tggcggcact cgtgatagtg tctgtatggc tgacacacgg
     1561 tatgcgagta agaaatccgt caaaaaaaca acttgtggca gaaaaactca ttacaatgct
     1621 ttacaatctt gtaaaggata caatgggtga aaaatatatg tcgttcgcac cgtatatagg
     1681 aacgcttttt atattttcga tagtcggaag tttatcgtcg cttacgggtt taagaccgat
     1741 aacggctgat ttaagcgtta tactttcgtg gtcgatagtg acgttcctta tgatacaagt
     1801 gacgaacatc aaaaatcacg gtgttttcgg ttggcttaaa tcgtttaccg aacctgtacc
     1861 ggttataaca ccgcttaacc ttataagtga aatcgcaaat cctgtttcaa tgacgttccg
     1921 tcatttcggt aatattgccg caggtcttgt tataacatca cttgtatacg gcggtttggc
     1981 gagtcttagc agtgttatac tttcgtggat accaaatgat ttctttgcaa caataccgat
     2041 tttacaagtc ggtattccgg caatactttc ggtttacttt gacctgttca caagcttttt
     2101 gcaggcatat ataatctgta tgcttacgat ggtgtttgtt caaggtgcag gcgactaaaa
     2161 aacaaaaata aaaaacaaca ataaatataa aacaaaggag atttaaaaaa tggaaagagc
     2221 aattatatta gcggcgtcgg caataggtgc gggacttgca atgatagcag gtgtcggacc
     2281 gggtatagga caaggttttg cggcaggtaa ggcggcagag gcagtcggca gacagcctga
     2341 ggcaaagggc gcaattactt caacaatgtt gtttggttgt gcggttgcgg aatcaacagg
     2401 tatttacggt cttgttgtcg cattgatttt attgtttgca aatccgctta taggacttct
     2461 ttaatcaaac agcgaaagga tgagagcaaa tgaatgaata tttgcctttt gtatccatag
     2521 acacatggac aatgattttt acgtgggcaa acctgctcat attgtttctg cttttaaaga
     2581 agtttttgtt taaacctgta acgaagatac ttgatgaacg cgcagaagaa atcgaaaatt
     2641 cgtataaaca ggcagaggaa accaacgata aggcactaag ccttaaaagt gaatacgaag
     2701 aaaaactttt gtctgcaaaa aatgaggctg acggtataat aaaatcggcg gttgaaacgg
     2761 cggacagacg ctctgaatct attgtaaatg aggcaaatga aaaagttcgg tatataatgg
     2821 aaaagagtca aaagcagatt gaacaagata agcagaacgc agtcactgac gcaaagagag
     2881 agattgcgtc tatggctgtc gatgcggcag aaaaaattat aggcaaaaaa ttaactgaca
     2941 gtgatgatga agaactaatt tctgatatta ttgatagaat gtaggtgatt tatatgtctg
     3001 caccacagac ttacggtgcc tcgctttacg accttgcaaa agatgagggg atgtcaagag
     3061 agattttggt tgaacttgag gggattgttt cgttatttaa cgaacaccct gcatatgtta
     3121 aaatacttga tgcacctcaa atcgaacgag atgaccttat ggagatactt aacgaagatt
     3181 tcttgggcaa ggtgaacaga tatgttttga atttcttaaa gttactttcg gaaaagcata
     3241 ttgtacatca catcggggaa tgttttaaaa catatgaaaa gctgtataat gaggacaata
     3301 gtataaaaat tgttaatgtt acaacagcaa agcctatagg caagcatttg gaagaaaaac
     3361 tattgcagaa acttgagaag aaaacaggcg gaaaagtagt tttgaaaatg cacattgata
     3421 aaaaatgtat aggcggaatt ataattgaaa cagatggtat gcggattgat tcaagtataa
     3481 aatcaggact tgaagattta aaaaaagctt tgatatagca taggagtaga agatgcagga
     3541 tttaagcaaa gttataaagg acagaattaa aaattacgat aatataatac gcgaaacaga
     3601 aacgggttac gttgtagaag tcggtgacgg aattgcgcgt atacacggac ttgatgcgtg
     3661 tatggccggt gagcttatcg agcttgacgg cggagttatg tctatggcac tgaaccttga
     3721 agaagataac gtcagcgttg ttattctcgg tgatgatacg ggaataaaag agggcagtat
     3781 cgcaaaacgt acgggacgtg tcgcagacgt tcccgttggt gatgcacttt tggggcgtgt
     3841 tgttgacgct ctcggaaacc ctattgacgg caagggtgca ataaatacaa ccgaaacacg
     3901 ccccgtcgaa tctccggcgt cgggaattat cgaacgtaag tctgtaagcg tgcctttgca
     3961 aacgggtata aaggcgattg actctatgat acctatcggc agaggtcaga gagaacttat
     4021 aatcggtgac agacaaacag gtaaaacggc gattgcggtt gataccatta taaaccaaaa
     4081 agatacggac gtactttgta tttacgttgc gataggtcaa aagcgttcaa cagttgcgtc
     4141 aatcgttgat acgcttgaaa aggccggtgc gatggattat acgattgtcg tttccgcaac
     4201 ggcgtctgaa atgccggcac ttcaatatat tgcaccgtat gcaggttgtg caatgggcga
     4261 atattttatg tataaaggca aggacgtttt gattgtgtat gacgatttgt caaaacacgc
     4321 agtagcatac agagcgttgt cgctgctttt aagacgttca ccgggacgtg aggcgtatcc
     4381 gggcgatgta ttctatttac attcaagatt gcttgaaaga gcggcgagat tgtcggagga
     4441 tttgggcggc ggttcgctta cggcattgcc gattatagaa acacaggcag gtgacgtttc
     4501 ggcatatata ccgacaaacg taatttcaat tacggacgga cagatattcc ttgaaacgga
     4561 attattccat tcgggcatta tgccggcggt caatcccggt ataagcgtgt cgcgtgtagg
     4621 cggtaacgca cagataaagg caatgaaaaa agttgccgga agtctgaaac tttcgtattc
     4681 acagtacaga gaacttcaag cattcgcaca gttcggctcg gaccttgaca aagacacaaa
     4741 aatgcgtctt gcaaagggtg agagaatagt tgaagtgcta aagcaaggaa gaaacacgcc
     4801 gataagagta tcattgcaag tggtaattat ttatgcggtt ataaacgatt tgcttgaaga
     4861 tatacctgtt gaaagaatac aggaatttga aacatcatta tttgagtata ttgaaaataa
     4921 tgcacatgat ataatagata cgattgaaaa cacaggtgaa atgaataaag aatgtgaaga
     4981 aaaaatccgt tcatctgttg aagattgtaa gaaacgtttt gtaaagtagg ggggatgctc
     5041 tgatgtctgc ttcatcaatg aagacaataa aaaacagaat aaagagtata agctcgacta
     5101 tgcagataac aaaagcaagg gagcttgttg ccgcgtcaaa aatgcgtaaa gcgtccgagg
     5161 gtattgaacg ttcaaagccg tattttaata tacttcacga aacgcttgaa gatattgcaa
     5221 aggataataa ggatttcagt tctgtattta cgcgtgaaag agagggaaaa acttgtcata
     5281 ttgttatagc cggtgataga gggcttgcag gcggatataa caacaatttg tttaaatctc
     5341 ttgacataaa agacggagat ataatttttc cgataggtaa aaaggttgtg gagcatttcg
     5401 gtgaagataa tgtgtatacc gacacatatg ccaaagcagg cgatataaaa atctccgatt
     5461 gtcacgcaat aggaagctta ttggcaaagg cgtatgaaaa gggcgaattt acgcgcctta
     5521 ttctttcgta tacaagcttt gtcaatatgc ttacgcaaga gcctaagacg gaaaatgttc
     5581 tgccgataag agttgagcat acaaaagagg gtatgcgtaa tatgaattac agccttattg
     5641 tgtatgagcc tgacgcagag gaaacttttg cacagataat accttattat atttccggaa
     5701 tggtgtacgg tgctgtgtcg gaatcggttg cgtcggaatt atcggcacga agaaatgcga
     5761 tggaatccgc aacggacaat gcgtcggaaa tgattgaaaa tctgagcctt gaatataaca
     5821 gagcaagaca ggcatcaatc acgcaggaac ttacagagat aatatcgggt gcgaataact
     5881 tataatccga aatttttccc gcagcactac tttttgtttg gagcagtacg cccgtacggc
     5941 accacaaact gcaaagcagc actgcgaaaa aaattacaaa ttataaaatt acggattata
     6001 tagagatgaa ggagttatgt atgaataacg ttggaaaaat agttcaaata ataggtcctg
     6061 tccttgatat aaggtttgag gacggtgaat tgcctaatat tttaaatgcc attgaaattg
     6121 acaataaagg tcaaaggctt gttgcggaag ttgcacagca tataggcgac aatgttgtca
     6181 gatgtatttc gatgggctcg acggacggac ttgtcagagg aatggatgcc gttgacacag
     6241 gtgagggaat taaagtgcct gtcggtgaag aaacactcgg aagaattttt aacgttttgg
     6301 gtgacgcggt tgataatatg cctaatcccg aaacaaaaga aaaatggtgt atacatcgtg
     6361 agccgccgac atacgaagaa caaaatccga caacggaaat acttgaaacg ggtattaagg
     6421 ttgtcgacct tattgcgccg tatgcaaaag gcggtaaaat cggtcttttc ggcggtgcag
     6481 gtgtcggcaa gannnnnnnn nnttataatg gagcttatca ataatatcgc taaggaacac
     6541 ggtggtatca gtgttttcgc cggtgtcggc gagagaacga gagagggtaa cgacctttat
     6601 aatgaaatga aagagtcggg cgttatcaat aagactgcgc ttgtttacgg tcagatgaac
     6661 gagccgccgg gtgcaagaat gagagttgcg ctttcgggac ttacaatggc tgaatatttc
     6721 agagataagc aacatcagga cgtgcttttg tttattgata atattttcag atttacacaa
     6781 gccggttcgg aggtttcggc acttatcgga cgtattccgt cggcagttgg ttatcagcct
     6841 acactttcta ctgaaatggg tgctttgcag gagagaatta catctacaaa gaacggttct
     6901 ataacttcga tacaagctgt ctatgtgcct gcggacgacc ttacagaccc tgctcccgca
     6961 acgacatttg ctcaccttga cgcaacaact gttttgtcaa gacagatttc ttcgttgggt
     7021 atttatccgg cggttgaccc gcttgaatca acgtcaagaa ttttgacacc cgaagttgtg
     7081 ggtaaggaac attacgaaac agcaagagcg gtacagcgta taattcaaag atataccgaa
     7141 ttgcaggata ttattgcgat tatgggtatg gacgagcttt cggaagagga caagaatacc
     7201 gtaaacagag cgagaaaggt tcagagattt ttgtcacagc cgttctctgt tgcggaacag
     7261 tttacaggta tgcaaggtaa atacgttccg attaaagaaa caattcgcgg ttttaaggaa
     7321 attatcgagg gtaaatgtga tgatattccc gaatcgtgtt tccttttcgc gggcggtatt
     7381 gacgaagttc gtgaaaaagc aaagaaaatg ggtgaatgat tatgaataca tttcatttgc
     7441 agatagtaac gcccgacgga cttatgtttg acggtgatgc ggaaaaatta cttgttcgta
     7501 caacggaggg tgacgtcggt attatgagcg gtcactgcga ttatgtaacg cctgtcgata
     7561 cgggcatggc aaaagttaca acaaacggcg atgtaagaac ggcggcgtgt tcgggcggac
     7621 ttttgaatgt gaaaaaagga acggtcagac ttgttgcaag tacgtttgaa tggtccgaag
     7681 atatcgacgt tgaacgtgcg ttgagggcaa aagaggcggc ggaaaagaaa attaaagaag
     7741 cttccgacta cgaagaaaga gccgctgaaa taaaattaaa aagagcactt acgagaatac
     7801 gtgtaggtga agaaaaatag taaaagggga cactaaaatt cagtgccccc tcagattgtc
     7861 aataaattgt ctggaacgtg caaaatatta ttcttataat ttaataaaaa acaaaaggca
     7921 agcctatctt tgatatacgg cttgcctttg cactttcagc gaaaataaac tctaacgtac
     7981 ctgtgtacgc cgacaagttt attttcgcag aatccaaact aattnnnnnn nnnntaagtt
     8041 gtgaaataaa attttgctaa tcgtattgac aaaaattaac ctctcggata tactaaaata
     8101 tgaataggag ggcaaggtat gcagattgaa aaatactaca atgatttaaa cacattgcat
     8161 attaatacag aagaaaacag agcgtattat atgccgtgtt cggatataga aaatgcaaga
     8221 aagggaacga atgacagaaa gcaggattta aacggtgagt ggaactttat gtattacact
     8281 tgcattgatg atgttcctga tgattttaag gacggaaata cagacggctt tgataaaatt
     8341 cctgtaccgt catgttggca aacgcaaggt tatgaccgcc atcaatatac aaatacaaga
     8401 tatccgtttc cgtataatcc gccgtatgtt ccgtatgaca atccgtgcgg tgcgtatgta
     8461 actcattttg aatcggataa tgattatagg aagtatttga attttgaggg tgtggattct
     8521 tgtttttatg tgtgggtgaa cggcgaattt gtcggttaca gtcaagtatc acattcaaca
     8581 tctgaatttg atattacaga taaggttaaa aaaggcgata atatactttg tgtgttggtt
     8641 tttaaatggt gtgacggaag ttatcttgag gatcaggata agttcagaat gagcggtatt
     8701 ttcagagatg tgtatatttt aaatcgtccg cagaagcata tccgcgacta taaaatcgaa
     8761 acagatattc aaggaaaaat taatattgaa atagattcgg acactgatgt gagctttgtt
     8821 cttgaaaatg aaaaaggcga gatatacagc ggggacagta aaactataac ggttgaaaaa
     8881 ccgatacttt ggaacgcgga atatccgtat ttatatacat tgtatcttgt aacggaagat
     8941 gaggttatac gcgaaaaaat cggtttcaga gaaataaaaa ttgatgacgg aattgttttt
     9001 cttaataata aaccgataaa aatgaaaggc gtaaaccgtc atgacagtga ccctgtaaca
     9061 ggatatacca taagccgcga gcaagccgaa aaagatttaa aacttatgaa acagcataac
     9121 atcaatgcac ttcgtacaag tcactatcca aatgctccgt ggcttatgca gttgtgcgac
     9181 gaatacggct tttatgttat tacggaatct gatattgaaa ttcacggaac agcatcattt
     9241 ttcggtggtt cacaagccgt tacattcgga ttgcttgcac aaaatcccga ttgggaaaat
     9301 gcaattttgg acagagttca gagaaatgtt atacgcgata aaaacagaac aagcgtgtgt
     9361 atttggtcgc tcggcaatga gggcggttac ggcgtgaatt ttgagaaagc aggcagatgg
     9421 gtcaaggaat atgactccac aagattgacg cattacgaaa gcagtatgtg gcagatggag
     9481 ggacatataa acgatacctc aatgcttgat gttgaaagta caatgtatgc ggattataag
     9541 tggatagaca agtattttga aaatccgggt gaagtggttt ggcacagagt cagtcttgga
     9601 aaaggcagtg aatacggcgg tgcgggcgaa tggataaatc tttcggagag taaactcggc
     9661 aagaaagaaa aggaacaaat ggcggttgta aaaccgtata tgcagtgcga atttatacac
     9721 gcaatgggta acggccccgg cggtataaag gaatatatag accgcctata cagatatgac
     9781 ggatttttcg gtgcattcgc ttgggaatgg tgcgaccatg ctattgatat gggggacagt
     9841 aaatattttt acggtggtga tttcggcgaa tatccgaatg acggtaaatt ctgcgttgac
     9901 ggacttgttt accccgacag acgacc
//
LOCUS       DAWACS010000009         9573 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_828_length_9573_cov_2.513763, whole genome shotgun sequence.
ACCESSION   DAWACS010000009 DAWACS010000000
VERSION     DAWACS010000009.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9573)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9573)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9573
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_828_length_9573_cov_2.513763"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1541..1550
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2560 a         2327 c         1649 g         3027 t
ORIGIN      
        1 gtctcaccat ttatgtttaa gacattcgca tccgtaattt gcattgttgt tcccgtaaat
       61 ttggaattcc acgccgtagt ctgtttttta aagccagtcg tatctatatc aatatagctt
      121 tcctctccca tatccttatg aggatatgta ttggcagtat aaacaggtgt agcgagtttt
      181 acaattttta cattatcaat agttatagat cccgtatctg ccgtagcggc tccaaaatat
      241 atgccggaaa gagcggcgtc aagcttaccg tcagtctgcg gattaccctg tttcacaagt
      301 tcatttatgc ctattgttac tgtttgaact tcagtgttta catctacagc tttagtccat
      361 acttccatat cagaaccata aagacctata tttacacttc cagcagttgc tgcggaaata
      421 tccattgtta ttgtctgaat tccatttaaa tcccaatcaa acgggaaatt cagtgttgca
      481 taccaattcg ccgccgcagg tgttatcgct acatttccgc tttcatcaga atatttaatc
      541 gaagccttac ccgatggagc agtatagctt ctgaatacat cgtctgtgct gaaatctgtt
      601 gaatatacac cgtcaagctt ttccggtgca gtgttgtatg aaagtaagcg attctttata
      661 tgagtaccgt tatatgccgt agtcttataa tcccatgaat agagcatttt cggcgatgaa
      721 ctcatatgca gattccatgc tgtccagttt acaggaattc cgtcatactg attgtcagta
      781 tcgtccatcc agttcataat ctgattcatc catatatcgc ttgtgcagtc tccgcccgat
      841 atgttcttgt cggatgagtc ccatccccat tcacctataa tcaccggtgc aacaagtctt
      901 accggaccta ttatcgtatc ccatgcggtt tttgcaccct taaccggata tgcgtgagag
      961 tcatacataa cgccatggcc ttccgccgta tctattagtc tatatccatt tgggcgttca
     1021 ttatagccat cagcaaaacc gcttatatca aatgcccagt tcagaccgcc ggctatacag
     1081 atattatttg caccttgttt gcgaatttcg tttagaagtt gttgatgacc tatagcagtt
     1141 acttcttcac cgccaactat aatttgtccg ccattatacc acacgtccca ttgttccact
     1201 gtagttggtt tttcaactcc taccggtttt atatcatgtg gttcattcaa aagaccgaaa
     1261 agtaccgcac tgttattgcc gtacttaaca gcaagttctt tccacatatc aagatcgtct
     1321 tgttgtggca taacatatct gtgacaatca agaattatat acttacctct cgcctgtgca
     1381 gctttaacca tatcatcaat atatttctga tattgttcct ttgttaagtt tttttcgtcc
     1441 catacgctgc cgtttttcca atatttagga tttattggca aacgtatcag attagctccc
     1501 cagctatcat agaccattgt catagactca aatagatgtt nnnnnnnnnn ccccagtcca
     1561 tactcggaac atttacacca cgaagaacta ccatttcatc tgttccctgc ttaactattt
     1621 tatttccctt gacctcaaga ggcacagaac gattatagtt aatttccgca ttttgagcgt
     1681 ctgctgcagc ttcctcatat gtctgtgcat aaacaggcat aatgcaaagc gacactgtca
     1741 ttacaatagc cgttacaaga cttaagaatt ttttcatgaa tatcaccctt tcgatttaat
     1801 tatataaaaa ggactgtttt tttgtaggga gaaagagaga gaatattgga aggtatccat
     1861 tgtccaataa ataacaccta ttctaaacag tcctttacta tattaacatt ataaaagaaa
     1921 agttcacctc aaaaaacgag gtaaactttt tctcactacc gattaattat gttttcggca
     1981 cattattcat attttaatgt ataaatttgt tttggagtaa cttcaacatc gcaccagcca
     2041 tttgtatcca ctgaaagctc tgtctctgaa agttcctcaa gcgtgacttt tttaacactt
     2101 gcccacttcc tattttccgt acacggaagt tcaaacgagt gtacctttcg ttctattggc
     2161 gattgtttat ctgatatctc cgctatgcca ccattaaatc taattctatt tttaactgtt
     2221 tcacttgacg gattaaataa tctcaccaca caccctaagc cgtcctcact gcgttttacg
     2281 gcacttatgt gtagattttc attttcaagt tcaataaacg acttttcaag agggttcttg
     2341 ccatgttctg ttggtgctgt ctgtccaatt agtatttcca tattaaaatc ttcagcagct
     2401 ttccatactt gagcatcttc ccaatcaccc ttatgaggca taaaagcata tcggaaagta
     2461 tgttcaccaa aagactgtga accgttctct attctcgaat agttctgctc ctcgggagtt
     2521 acatatatgc gaagctcaaa gcagcgcaat aatgacaaat acacagtatg gttataatca
     2581 tcatccgatt catacgcttt aagtcccgta ttcaaaatcg ccgcaccttc attttcattg
     2641 catatatcaa caaacgagtt catcggctgc tctgtcatcg gaatttcgtc atacttcgaa
     2701 taatccggtt tcgcaatcgg atgctttacc acatcaaact gtccctgagc gtatacaaac
     2761 tctgcatcca cgtctgtggg gaaagcagcc tgaagatagt gattaggaac attgttatta
     2821 attttcgttt caaattcaac ccatcttgcg ccttttctga gtgttacaag cgtttctatt
     2881 ctgtaaggcg cgaggcggga acttcttttc ttgccgccgt caactatatt ttcgggaatt
     2941 gcccaattaa gaactattct gtacttagtt tcaagctcac cgctgtatac aaggcttaca
     3001 atcgcccgtt cattaacagt tgtatattcc tcatcaagct ccggtgtctt gtgttcccac
     3061 gggctgccat tttcaccggt gtccttaaaa tatccgatat tatcgtattc cctgccggtt
     3121 tccttttcag taatcttaag cgtaccgttt gaatttatct taaccttcag atattcattc
     3181 tccatagtat ttatgccgca aagcatatta accggcgttg tagcccgtgt atgatactta
     3241 ggtacaacct tgagtgtttt atagcccatc gacggaatat ccttaacaaa tattcttatt
     3301 gtatgtcttg acacaggaag aacatcgacc gcgtctgcca aatcctgaac aatttgatac
     3361 ataggattaa ttgacgatat gttctggtgt gggcatacat taccttcagc atcgacaatc
     3421 tcaaagctgt cacactccca ctcaagagga atttcaagct cacacggaac cgtcagactt
     3481 cttttaaacg gagccggatt aaacattaca agcgccatat cattcttatc ccagcccgca
     3541 aagtcaatgt cgccagacaa atccataaga gctctttcaa gcacgcaggt tgcaatctct
     3601 cttgactgcc tgaaccgata ttctacgtcc ttatatacaa catctctgcc gcacgcgcca
     3661 atcgaatcat gcccgtggtt ttgaagcata tagttatagg ctttattaat aaacgcctgc
     3721 ggatatacag ctccgcatac agacgcaaaa accgccatcg gctcagcata tgatgtgagc
     3781 aatcgttctg tttcaaagtt ctcctgctta accttaattc ttgcagaaag aacccagcca
     3841 aacagcgcac ttacgctgcc ttttgtaaac ggatagcgca tttcgccctt taaaacaggt
     3901 gaatttttat caaaatctct gatcacgctc tgctcaaagt cataaactgt actgtgaaaa
     3961 acatcaacac cttcataaac agcattagca tccttgataa gccttgattc tctcatatcc
     4021 ggtattgatg aatcatgacc gttcgaccag aagcggttag gcgttgtcca ctcatcgtcc
     4081 tgttcagaga gtgcctgctc cgtcttttca gctatatatt cgtcatgata ctcatatttt
     4141 ctatgcgaat actgatattc atattcacat cttgccggat ccgcaaaacg gaatattcca
     4201 tctccagctc cccatgaaac acgacgattg tcaccgtcac gcttgccata aaacacaggg
     4261 cgctgcatta tataccacat attatatctc ggtctctgcc ccagtcttga agcataaatt
     4321 gttgtaccgt cggcgccctc ccagtagaac tccgatttcg gtgccatata tgtattaagc
     4381 cctctgtaaa aggacgcaaa atctattccg aagccatgat atagctgtgg catttgtgat
     4441 atttgtcccc agccaaaggg cgaatagcct gtctttgaaa ctttgccaaa ttcatttgca
     4501 attttatgtc ccaggagaag atttcttata agagactctc cacccacgca aaactcatcc
     4561 ggcaaacaaa accacggacc cacagcaagc tttccctcac tgatatactt tttcagaatt
     4621 tccttctttt cagggtttat ttcgagataa tcctgaatgg gaagtgtctg cgagtcaaga
     4681 tgaaaatgtt tgtaatccgg ctctttctca aaaatatcga gcagcatatc aatcgcagtc
     4741 acaagcatat gtcttgttct ttgagcgctg aatttccact ccctgtccca gtgggtattg
     4801 gatataaaat gacattttat attttttcgc tccatttacg cttcctcctc tatgtaatca
     4861 agtatttccg caagattatt gcagtagcgg ctacacatag gcatcgttcc gctttctttt
     4921 atattgcctc ccgcaagtcc tattgttgta tatccggcaa tccttgccga gcatactcct
     4981 gcgccgctgt cctcaatagc gattacgcta ccgcgctcat caaaaccaac tccgagtcca
     5041 accgcgcagg tttcagcata aagccacgga tgaggtttcg gcgatagctc accgagcgtt
     5101 ccaacgctac ccttgcggag cgggtatcct gctgaaatta ttgcgtcgta aaaatccgta
     5161 ggctcgccca tatcaagagc tctgaacgcc gaaagtattt ccggcatcgc cttttcatat
     5221 aatcccgatg ttacaagtcc tatcttaatc cctttggctt ttagtgcgag caaaaattct
     5281 tttaatccct cctgcggaac aaaagcattt tttctgcctc tgccctccat aatttctttc
     5341 atttcacgat taacgtggtc aaaatagaaa ttccgcgctt tgtcaagcga ttcacctgga
     5401 cagtatttat ctatacaata ctgtaaatgc tccgatacgc tatgacctga tacaaacggt
     5461 atatcgctct cttcaagctt gaagctttca tcatcaagca tactggcggt tgttttttcg
     5521 attatccaaa tccagaactc ctcgcttctt accgatgttc catccaaatc cataagcaca
     5581 gccttgattt taccgtttct gttcacagga tgaagcggat agtacgcacc atagccaaga
     5641 gctgaattaa cataggcaag agtctgattt tcaaaagcca caaattcaac ctttccgtct
     5701 cctgtgcata ctatatgttt tactccgtcc tcaccggagg taaatcttcc gtctgtagtg
     5761 gagggaaggt tgcgaaatcc tgagctgccg tctataattc ccaaatcggg tatattgaaa
     5821 ttcattacat taccacctct acattgcata tttccgcatc cgatgaaacg gttgtaccgt
     5881 ttaccgttcc gttgtcggct gttattttaa cagttccctc tcctgtgttc tttactaaaa
     5941 tattatatgt ttttcctctg aattttcttg ttaccttaaa ttcagatata tcttttgaaa
     6001 tacacggctc tattacaagt ccgtcaaaat caggacgtat acccaaaaga tattgtgaca
     6061 ccgtataata attccacgcc gccgtgcctg taagccatga attcttcgct tctcccgctc
     6121 ttaccgcatc acgtccagca atcatctgtg aatatacata cggctcggtt ttgtgaattt
     6181 cacttatctc ctctaaatat gccggcgcaa tctttttata tagttcaaac gctctttcgc
     6241 cgttacccat taccgtttca ccgattatta cccatgggtt attatggcag aacactcccg
     6301 cattttcctt ataccccggc ggatacgacg agatttcgcc aagctcaagt cggtatcccg
     6361 aatacggcgg ataattgagt acaatgccat attcacattc aagatatttt ttaacgctgt
     6421 caagcgcttt ttgcgccctg ccgtcctcct tgcctatccc agccattaca cagaagccgt
     6481 tcgattcaat aaagatttta ccctcgtcac attcgtcaga gccaacctta ttgccgttag
     6541 cgtcgtaagc tcttatgaac cactcaccgt catagccata ctctaaaact gcttcggtca
     6601 ttttttcaac ctcatccgaa atatatttat acatttcatc attgttgagc gtcttataaa
     6661 gccttgcaaa ttctctgcca atatacacga acattcctgc aataagcaca gactctgcta
     6721 ctctgccgtc atcgtctccc gtagtctgga aagattcgtc aggaatttcg gaaaagcagt
     6781 tcaaattcaa gcaatcgttc cagtccgcac gtccgataag cggaagcccg tgagggccaa
     6841 ggttgtttgt aacgtgtccg aatgagcgat taagatgttc taaaagcgtt gccgtattgt
     6901 ttttgtcaca gtcaaacggc acctgctcgt ctaaaatacc gtagtcgcct gtttccttga
     6961 tgtacgcaac cgtgcctaaa atcagccaca gcggatcatc attaaatccg cctccgattt
     7021 cattattgcc ctgctttgta agcggctgat actgatgata cgcgcttccg tcctcaaact
     7081 gcgttgaggc aatgtcaata atcctctctc tcgccctttc ggggatttga tgtacaacgc
     7141 caagcaaatc ctgatttgaa tcgcgaaatc ccattccccg gccgataccg ctttcgtaat
     7201 atgatgcact tcttgacata ttaaaggtca ccatacactg atacggattc catatgttta
     7261 ccatgcggtt aagcttttca tcgtttgact caagagtaaa caccgaaagg agattatccc
     7321 agtacaattt aagtttatca agctccgcgt cgcattgtgc tgatgattca tatcttgcaa
     7381 tcatttcctt tgcctttgtc ttattgatta cattaaggct ttcaaatttc tcgtcctttt
     7441 cattctcaat atatccaaga acaaatatgt attcgcggct ttctcccgcg tcaagtgaca
     7501 cgtcaatctg atgagatgcg ataggatacc agcccgaagc tactgaattg ccgctcttgc
     7561 catttattac tctgtcagga gtgtcaagac cgttaaaggc tccaaggaaa gtgtctctgt
     7621 ctgtgtcaaa accacttatt tcggtattta ctgaatagaa agcgtaatgg tttcttctct
     7681 cacggtattc tgttttatgg tatatagcgc tgccgtcaat ttccacctcg cccgtattga
     7741 ggttacgctg atagttaagc atatcgtcct gagcgttcca aagacagaat tcaacgaatg
     7801 aaaacagatt tatgttttta gcctcaccgc ttgtatttgt caccttaatt ctatgtattt
     7861 cgcagttatc gtccacagga acaaaagctg tctgctcaac gcggacgccg tttcgctcgc
     7921 cggtgatttt tgtgtaaccc ataccgtgac gacactcata aaaatcaagc tcttttttca
     7981 tcggcatata cgagggtgtc cagcaatcgc cattatcgtt tatgtaaaaa tagcgtccac
     8041 cgttatccgc agggatattg ttgtatcggt atctcagaat tcttctgtgc tttgcgtcct
     8101 tgtagaagca atagccgccg gaggtgtttg aaattaatga gaaaaatccg tttgttccaa
     8161 gataatttat ccatggaagc ggcgttctcg gagtctctat tacatattcc ttattcaaat
     8221 cgtcaaaata tccatatttc atatttgttc tccttaccaa taaattttcc aatcgctttt
     8281 tttcatgcgc attaatgctt caagatagaa gtagtctccc caacttgtac attccggctc
     8341 atgaccgtgt tttctgctgt acataccgtc ttttataatt ccgttgcttt gaggataatc
     8401 tactgtcgtg tacttttccg aaagacttgt catcatcttt tccgccgcat caaggaattc
     8461 ctgattgtga taatattttt ccatttcaag aattccgcac accgcaacca ctgctgcgga
     8521 ggtatcccgt ggttcatcgc taccatcgga gaaaatcaaa tcccaatacg gaacagaatc
     8581 ctccggaaga tggtcaataa aataatgcgt aacccgttca aacaacggca aaatcgactt
     8641 ctctttagta tagtggtaac atagagcaag accatatact gcccatgatt gtcctctcgc
     8701 ccaactgctg tcatcggaaa atccctgatg agtttctccc cgaagcggct tattggttac
     8761 aggatcaaag aaaaaggtgt gatatgaaga cgcatcagga cggataatat ttgcgattga
     8821 tgtctgcata tgattatagg cggcgtcata atactttttg tagcctgtca cttcgctcgc
     8881 ccagaataga agcggaatat ttaacataca gtcaacaata aatctataac tttgcgaatc
     8941 atccatagca ccccacgcct gaataaattt accttttggc tgataacgct tcaaaagcca
     9001 ttctgccgcc tcaattccat cctgcttcgc ctgttcatct cctgttattc gataatcggc
     9061 gacgcttgaa agtgtaaaca aaaagcccat atcatgatgc tccagttcaa ctcgatcaac
     9121 caacctctta tgaaacattt cactgtgatg ctttgccgaa ttataaaaag ctttatcacc
     9181 tgtaagctca tacataagcc ataatatccc ctcataaaat ccggtagtcc atgaaacgtt
     9241 ctcaaatttt ttgaacacaa gattttcact ttgttctgta ggaaaacaat catagaaata
     9301 atttaagctt tttcttacga tgctctccgc atatgtaatc gctttatcaa tattcacttt
     9361 ttatcactcc tcctactata ataaatttat gcgtaaatca cacgacagtc attcgtgtat
     9421 atatccatct tgttgcttaa actgatataa tgaaagtgca atttctgacg tatcacgcct
     9481 cggattttct gtctgtagat aagtcagtct ctcatttgtt gtgttcgatt tatgcagtag
     9541 ataaaaggag cttttatcca gcccttttta tta
//
LOCUS       DAWACS010000010         9504 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_837_length_9504_cov_3.386284, whole genome shotgun sequence.
ACCESSION   DAWACS010000010 DAWACS010000000
VERSION     DAWACS010000010.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9504)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9504)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9504
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_837_length_9504_cov_3.386284"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3170 a         1723 c         2356 g         2255 t
ORIGIN      
        1 gatgtagcta attatacaga tacggagaat agggttgagc ctgaggagaa agaagctgat
       61 gatatgggaa taaatccacc tataaaaacg atatatacag ctgacccgtc agcacatgta
      121 tggaaaacag accccgacaa gctgtatgtg tatccatcgc acgacagata ccctcagcaa
      181 ggctgtaaca gaatggacgc gtttcatatt tactcgacaa aaaatatggt tgactatact
      241 gatgagggcg agattttcag tgcatacgac cttgaatggg gcagagaagc cttcgacaaa
      301 gacggtaagt ttgacgacgg cacgtttatg tgggcgcccg acgcggcata cagcgagaaa
      361 acaggcaagt attatttcta ttatccacgt cccgcgtcaa gagcatttaa tgacgagtgg
      421 atggtaggag tggcggtaag tgattaccct gatcacggct tcgttgatgt cggcgtgccg
      481 attattggaa taggcggcag gggaatgatt gacccctgcg tatttacgga tgatgacggc
      541 agaagctata tctatctcgc tggaggcgga acatggatga atggctgtgc ggttgcagaa
      601 cttaatgacg atatggttac gctcaaggag gttccgaagg ttatgatagt cgagggaatt
      661 cctgaagagt gggatgaaaa cggtgtgtat ataggaaagg atgaaaacgg aaatctcata
      721 aatatgggcg gtattcctga ttatcacgaa ggaacttatg tcgtaaagag ggacggcgta
      781 tattacatga tgtatgcgga cgcgtatgta cgcaatgagg acaatgaata cggtacaaag
      841 ggagctcata acagacagcg atatgtctat agcactgtaa gtcctaaagg accgtggaaa
      901 ataccgacag gcgacaattc cttatcatca gagccggacg gtgcaaaggg ggtaatactt
      961 gaccccgaaa gctcggacac ctcgcatggc tctattgtgc agtttaagaa tcagtggtat
     1021 atgttctacc acacaatgga tttatcagga aacggtgcgc tccgctcgtt ccaggcggat
     1081 aagcttacat tcaccgatga taccattgac aaggttatac agacagatgg ctcggaactg
     1141 cttataggcg accgcgagat aatagcggat gctgatgtgg tatatgaggc ggaggacgct
     1201 gaatttgcca atacaaatac ggaagccgcg cagcctaaca cggagaaatt tgaatataac
     1261 ggaaatcaga ttaccgctgt tacaaatctt atgataccgg gtgcgtacgt tgcatttaac
     1321 aatactgacg gaggagaaaa tggcggcaga gtaaatatca agctgaagta ttcggcaaaa
     1381 acacaatgct atgcaaagct tattgtaaat ggacgcgatt actcatttat aaatctaatg
     1441 ggtacgggcg gcaagggctt tttcaagggt gagtcaaata ttaccgtaac aatgaagccg
     1501 ggagagaata atacaataag tcttacagag tttaacagtg ctgtttcgct tgatagtatt
     1561 gaattgagat atttggacaa atagttcatt tttaaacagg gactgacgca gaattaatgt
     1621 gaaagtcctt gttttattac aaattaagct gggcaaacca gctataaaaa aatgctgatt
     1681 gacaaagtta acatgtattt tttatatcaa tttttaaaat ttgatataaa aatagtaaaa
     1741 tgcaataaaa cctattgaca aatttgatat atcatagtac aatataatca tataaaacat
     1801 aataaatgat atatcaaaaa aacaaaacgg aataatgcag ttttgcagta aggaggaaat
     1861 atgagaaagt ttattaaatt tattgcggca gcatgtgcgg cggctatctt tacgggtagt
     1921 gtttcagccg tttcatttgc atctggaaca ggaacgaaaa gtgtagctat agccgcacag
     1981 gagggcacta aatcagtaaa gattactaca gaagagacta taccatttga cggtgtagtt
     2041 atttacgcgg tttatgaggg gcagacgctg agctctgtgg aggcaaagac gtttaaggat
     2101 atttcctccg agaaagaagg agaagttcaa tttgataagg attatacaaa aacaaactcc
     2161 aagctttttg tgtggagcag cctttcagaa atgataccgt ataatgtaac agttacaccg
     2221 gagaaagtac agataaacac agtggcaaag gttgagattt cgggtgataa aacagctccc
     2281 gcgactttaa atgcaactgt atcagcaata aatgattctg gatttgaaga tacatccgct
     2341 tttatatatg aatggaaaaa gggtgaggaa attctttcgg aaacaggatc aatacttgaa
     2401 gatgccgcag aaggcaatta tactgtaacg tcaacctata ccggtacagg caattacgaa
     2461 ggcagtataa caatcgcagc tccgtatgtt gtaagtccgg agggagatac aagagcggat
     2521 attaccggcg atgataccgt aagcgtatca attaatgctt catcacagac ggctccgtca
     2581 aatctcactg cacagatttc agccggcgaa acggctctgg cagtaaatga ctttacatat
     2641 gtttggtcgc ggaacggctc gcctatatcg ggcggcgaaa acggcacgct tgaaaatgtg
     2701 caggcgggtg aatataccgt agttgctacc gttaaaccgg ataatgcaag ctacaaggga
     2761 agtacagcgg cttcacaggc tgtaaccgtt aaggcggttt atacagtaac atggagtgta
     2821 gaaggagata ctacgcagga aacttacgtt gaaggtgaaa tgccttcata cagcggcacg
     2881 ccttcaaagg atcagacaaa tacagataca tatgaattca cgggatggac tccggaaatt
     2941 acagctgtaa cgggtgatat aacctatacg gcgcagttca gcgcatcaaa gagaagctac
     3001 aacgtaacct ataatacgaa tgggggtact attgcgaatg aaacaaatta tacaagctat
     3061 acctgcggca cggcgttgac acttccgata ccgacgagaa acggttatac cttcggcggc
     3121 tggtatgaag aaagcgattt taacggcaca ccggtaaaga ctattgacgc agaggcttat
     3181 ggtgataaag aatattttgc aaaatggaac tctaatgagc ctgataaatt tactatcaca
     3241 tggaagaatg atgatggctc gacaattaaa accacacagg tctcaagcgg cgtaatgcct
     3301 gtgtttgacg gcaataatcc tacaaaggct gacactgcgc aatatacata tacctttgca
     3361 ggttggactc cagaagtgat tgcggcaaca gcagatgccg aatatacagc tacatttact
     3421 ccgaccacaa gaagctataa aatcacatgg gtagttgcag gtaaggagaa taaggaagag
     3481 gatgttgaat acggtgtaac tcctgaatac ggcgaaatgc caacaagaga agctacggca
     3541 gagtacacat atacctttaa agaatggtcg ccggaaattg cagctgtaac agggacgcaa
     3601 acttacactg caacgtggaa cgaggttaag aacaaataca ccgttacatg gaaggatgga
     3661 aacaatacac ttaaaaccga acaaatagct tacggtgaaa cacctgaata cagtggcgat
     3721 gcaccgacaa aggaaggcta cagctataca tggacgcccg agataacgga ggtcacaggc
     3781 aatgcgacat atacaactaa ctggacaatc aataagtata cggttacaaa taattctgca
     3841 actgatgacg atggcacaaa gcacggtaca atcacactca acggacttga cggtgatgga
     3901 aaggcggagt acaatagtac tataaaagta actccttcag cagccgaggg ttatgaactg
     3961 aaaaagataa ctgtaaacgg tgcagatatt acaaaaccgg taaatggcgt atggcaattt
     4021 actattcccg catcagatgt cgcggtaact gcaacctttg agaaggctgt taagtccata
     4081 actgtagagg gcgcttcgcg tgttcaaaag ggcaagacgt cgaattatac cgcaaaggtt
     4141 acagcggtgg atgatacgga tgttacagcc gtatatgcat cggatataga atggtccgtt
     4201 gatgaagcca gcacaggggc aggaactaca atagaaaacg gcactcttgc cgtaaacagc
     4261 gctcagacat cggaaattac cgttacggca aaggttggag aagtaagcga cacagcaacg
     4321 gtaactccga caactcaaag acctgacgca ttgaaaatag tttcggctga ggatataact
     4381 aatcatctga gtaacaatgc agtagcagta aatgcaatag acggtgatac agcgaccaat
     4441 tataatgcaa taacgcaaga tccgtcagaa aatccgacag gaagaacgca gacatatatg
     4501 acagttgatt tgggtgctgt taagaaagtt tcaaatgttc agatttggtt ttatgcagga
     4561 aatgcaagaa aatacaattt tgatataaga gtaagtaaag atggaattta ctgggaaaag
     4621 ataaatacat ttgaaagcag tggtactact gcaaacggtt ttgaaacatt tgattttgga
     4681 aagacggtga gcgtgcgtta tgtgcgttat gtcggacgcg gggcatatgc aaatgccaat
     4741 agtacattca tccaatacaa tgcaattcaa gaatttgaag tgtatggttc cgatacagca
     4801 actgatgcag cagaactgct cacttcatcg gatacgcaaa ttacagccag cgcatatcta
     4861 aatgcagatc aggcgccttc aaaggctgta gacggcgata aaactactca atggaatgcg
     4921 atgatgacaa atacagaacc aaacacatta actattgact tattggcaaa gcagtcagta
     4981 tcagcagttg aattgctgtt ctatcagcca tggataaggt cgtataagtt taaaattgaa
     5041 gtaagcgctg atggctacga atggattaca gttgccaact atgtcgaaaa cggtgtcgga
     5101 tatacggtag gagaagcata tgatgagggt gaacgaatta catttaccgc acagacagcg
     5161 cgttatattc gatttgtagg ctatggttca acgttgaata atggaactca aaatacttac
     5221 aatcctgttc gtgagttcag agtgtacgga acaggtccta aatctttaac tctaactgca
     5281 tcggcagaaa atgagcatgg aggaaccact gcgtcatatg cagtagacgg tatcattggt
     5341 aagcttgagg atactactac tgattcccgt tggttggcaa tgatgataac agacaccaga
     5401 ccagaaaata aaataacctt tgaccttggc gaggaaaaga aaatatcagc tgttgcaata
     5461 tcattctatc tgggaaacgc aaggtcgtat aactttgaca ttgaggtcag ctccaaaggt
     5521 gatacttgga caaaagtgct tgaaaataaa gatagcggcg caaaggtcga taaagaatat
     5581 gaagcctttg agtttgagga aacaacagcg cgttatgtgc gctatatcgg acgcggtgcg
     5641 acagagttga gcggtggcgt gcataacaat tacagcagct tctgggaatt tagagtatat
     5701 ggatatgaca acgagtaaaa aggaggaaga atgatgaaaa atatctacaa agcggttgcg
     5761 gcggtatgcg caatggcagc tcttatgagc agcgtgcccg cgctgcctgt gagcgcggat
     5821 actgtaacaa agcctatgta ttatgctcag gcggatatgg gtgcggcgac agattttaac
     5881 agagttaaca ttgactttgg aggcagcgcg cctgatgaat acaccgttga aagctcaatg
     5941 gacggcaaaa tatggactga gcttacaagc aatgttactg aaaaaaacgc ggctgctgat
     6001 ttcagcttca acgaagctaa taattgctac gtttacaagg attataacac acgctatgta
     6061 agagttgttt cggatgaaga tatgtcaaaa gcgacaataa aaataagcaa aacaacagac
     6121 attgctccgg agggaagctc aatgctgttt gaaaaaaacg acggtacaga cataaaatcg
     6181 tcaaccgacg tcgtggatgc aagacttaac aagaaggata tttctgcctg tgcatatatt
     6241 tactggggaa gtgcaagccc tgaaacatat gattttgaag aagctaatat cataattccg
     6301 cttggagaag aaaaagagat ttcaagattt gagataggct cgtattttaa tgataaaacg
     6361 aaggcttttc aaaacttctt tgcaaagaat tatgaaatct catatataaa cagcagcacc
     6421 gaggtacatg caaatacaga aggctggaaa ccacttaaat ccggaaatac tgcttcaggc
     6481 tggctgactg ccgcatttaa tcctgtaaaa gcaactcata taaagttcaa tacgaacgac
     6541 aagggagttg caggaagaaa taacggcaaa atcgcaacat ttttaagcta cataggagta
     6601 tatacctctg ccattgttcc gtcagatatg acatatggaa gctatgacac tgaggtggaa
     6661 gaagtagagg atatgggcga ggttgacctt gtaatgttta tggggcaaag caacatggca
     6721 ggacgcggag aggcggcgga cagtgtggtt tgtcaaagcg gacacggcta tgaattccgc
     6781 gcgataagcg accctgcaaa gctgtatgat gtaagggaac cgtttggagt taacgagaat
     6841 aatgcggcga gcggagttaa cgagctgggt tcaaaaagcg gctcgatggt atcggcgctt
     6901 atggaggaat attattccta taccggagtt cctatggtcg gagtatcatg ctcaaagggc
     6961 ggtagtcaga taagcttttg gcagcccggc agtccggcgc ttgacgatgc tgtttcgcgc
     7021 tataaggcgg caaagctgtt tcttaaaaag aatggctata ccgtaagacg caagcttatg
     7081 gtatggtgtc agggtgaaag tgatgcagat gtgtcaactc ctattgcgga ctataaggaa
     7141 atgacgcaga atatggcgca ggctatgata gacgaaggca ttgagaaatg ctttatggta
     7201 caaatcggac acaagaatga tacaagcggt aaatatgacc catatattga ggctcagtca
     7261 gaactttgcg ctgcagatga tcgtttcgta atggtatcgg aaaagtttgc cgataatcag
     7321 gcaagtatga aggatttata ccattatcat cagtcggtat ataacgaagt cggcgcagat
     7381 gctggtaaga atatagctgc ttactacgga gatacaacaa aaaatcttcc gtcaactata
     7441 aatataattg ataaactgag aaccaatacg gaagcgggag ttacaataga cggcgatacg
     7501 gacggtgttg taattgcagc gctttatgac aaaaacggcg cacttgaaca gagtaaaatt
     7561 gtagataaaa ttgacaaggc tactacagta aaatttatca cttcggaaaa cagcaaaaca
     7621 attaaacttt tcaactggaa tagccttagt gaaatgcagc cgataggtga gagcaaaagt
     7681 ataaatataa atggaataga tataaaaacc tcggcaacaa caagcgactt cctctatgga
     7741 agctatgagg acagtacaaa gtacaaacat gctatgccgt acagatatat cctgcctgtc
     7801 aattatgaca gcagtaagga gtatccgata cttatgtatc ttcatggcgc aggcagacgc
     7861 ggaaacgaca atgaaaatca gttaaataac ccgaaaccgc tgtttgacag actgttgagt
     7921 gaggagaaca taaacaaata tccttgtata attgtcgctc cgcagtgtcc tgaaggtgag
     7981 caatgggttg acacaccatg gggcaaggga acatataagg taagcgaagt tcctgtaagc
     8041 gatgagctga gtatggcaaa agatattatt cttgattttg aaaatcgctt cagcgtagat
     8101 actgacagag tttatatagc cggacagtca atgggcggct acggaacatg ggatatgatt
     8161 atgagaaacc ccgatatgtt tgcggcggca ataccgcagt gcgccgcagg tgatgtgaca
     8221 caggcggaaa gtcttaaaac aatggctgta tgggcgcatc acggtgaaat tgatgacacc
     8281 gttcctttaa gcggctcaag ggaaatggtg gcggctctta aggaggcagg ctcaacgaat
     8341 gtgagataca ctcagtatcc aagcataagc catgaggtac agattgagac atttaaggaa
     8401 ccggatttgc tgtcatggtt attctcaaaa agcctgacgt caaatgcaaa tgttaaggat
     8461 gattacactt atcttaaggt taatgatgtg cttgtaagag agtatacaga ggagaatcca
     8521 agacctgtta agcaggacgg taaggtatat gtaagcgcag aatatatagc atcagagctt
     8581 ggtatgacat atgagaacag cacaatgaca aagggtgaaa aatcactcac tcttactgat
     8641 ggaaacagca taacatcaga tggcgttgtt atggttgact cggcagcttt ggcggaaact
     8701 cttgggcttg caagagagtt tgacgaggtg tcaaatacgc tttcgttcaa agcgaatacc
     8761 cagcgtaatg gagcgcttga aattaaagaa gttacagcga gctcacgtct tacagacgga
     8821 aacgagcagt atgcggcaga cggcaagcct gacacgcgct ggacggcgat tgtacaaggc
     8881 gctgaaacaa ctaacaccct gacagtagac ctcggtacaa taagaggtgt agagaaagcg
     8941 tctattatgt tctatctcgg caatgcgaga aagtatgatt ttgatataga ggtcagcaca
     9001 gacggaagta catatacaaa ggtaaagacc tttacaagca gcggtactac aaacgacttt
     9061 gaggattttg tatttgatca acctgtaaat gcgagatgtg taagatatgt aggacgcggc
     9121 tcgacagtag tggctaatag ttcacataac gattataaca gcttctggga atttgagata
     9181 tatggttcag aagagccaat agaggatccg aatgcacctc aggtatttaa ggagctgaaa
     9241 ataaactcgg cagaagcaaa tgcagagagc attgattcag gcaaaggcat aggttatgct
     9301 tctcaggcga tagacggtat tgtaactgat gcaagccgct ggtctgctat ggtgacaacg
     9361 gcaaagcctg agaatacggt aactttcgat tttggagaaa taagatgtgt cagctccgtt
     9421 gatattgcct tttatgcatc gtcagcaaga aagtatgatt ttgatataga ggtcagcaat
     9481 gacggacaaa aatggaagaa agtt
//
LOCUS       DAWACS010000011         9392 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_846_length_9392_cov_2.862375, whole genome shotgun sequence.
ACCESSION   DAWACS010000011 DAWACS010000000
VERSION     DAWACS010000011.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9392)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9392)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9392
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_846_length_9392_cov_2.862375"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    569..578
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2636 a         2116 c         1867 g         2763 t
ORIGIN      
        1 accttccgaa ccgaaaagta taactttttt atcaaaagtc gcaagaccct caacttctgt
       61 cggacggccc actcctgtaa atactgctaa ggtaacttta aagaaatact tcaaatcaac
      121 cgaataaaaa cctctgttaa acggcacagc ctcaatgtcc gtaaacaccc aaatcacttc
      181 ggcttttgtg catttaacgt ttattgctct gtccacaata tcctgaccgc acgaagtcaa
      241 gtacacgcgt acattttcca agcagtcttt atccctgcaa ctgtcatata tcttatcagt
      301 atgtacacac actgcttcac ggaaatttgt atcacaagga tattttcttt ctcttccgca
      361 gtcattacag caatttctgt tacaatcagc catttattat cattcctttc tatgtttttg
      421 gggttttccc ttggtataga atatgaaact caccgaaaag tgttactttt accgattatt
      481 ttaaattaaa gtctgaaaat tgcaaacatt tctatttgga ttatgatata atatgaaaag
      541 tttagaagta tcacgaaagg aattaaaann nnnnnnnntt acggtatatt ttttgaagac
      601 ataaaccatg ccgccgacgg cggactttac ggtgaaatga tacgcaacag ggcgtttgaa
      661 ttttcaccta tggacaatcc gtcatatcag gcgttgacgg cttggaaacg tattgaagag
      721 ggcggtgcgt cggtatcgtc atttgtatca aacaaatcac cgttttcaaa gcgaaatccg
      781 aattatctta ttcttgaaat taacaaagca ggcacaagag ccggcataaa aaatctcggc
      841 tacaacagcg gtattgcggt aaaggaagat gaaagctaca atttttcatg ctacgcaaaa
      901 agtgacaagc cttgtgaaat aacagtttca atagacaacg catacggtga agtcattaca
      961 gaaaagtcgc ttaacatcac gtcaaacgaa tggacggagt attcatttac attaacatca
     1021 cctgttgacg atttgtcggc agtgcttgca gtcacatcaa agcaagaatg taaattctgt
     1081 cttgattttg tatcgttatt ccccgtaaaa acctacaaaa accgtaaaaa cggtatgaga
     1141 aacgacatag ccgaaatgct tgcggactta aagcctaaat ttatgcgttt tccgggcggt
     1201 tgtcttatcc acgacggcac acttaattcc gacgacagaa actcaatgta ccgttggaaa
     1261 aacacaatcg gtgctgtaac cgacagaccg tcacgacgta acaactggcg atacaatcaa
     1321 tcgttgggac tcggatattt tgaatacttc caattctgtg aggacatcgg tgcaaagcct
     1381 ttgcctgtac ttcctgccgg atataatccg catatggagc aagccgttcc gcttgacgaa
     1441 atgcaggaat ggattgatga cgcacttgac cttatcgaat ttgccaacgg cactgccgac
     1501 acgaagtggg gcaaaataag atgtgatatg ggacacaccg agccgttcaa tcttgaatat
     1561 cttgcagtcg gcaacgaaga agtcggtcag ggcttttggg acagatatga ccttttccac
     1621 aaagccataa aagagaaata tccgcaaata aaaataatca actctgccgg acctttccca
     1681 caaggcggtg aatttgaacg cggttggaac aacgcaaaaa agaacggctc tgaccttgtt
     1741 gacgagcatt actacacatc acccgaatgg atgcttgcca actgccaccg ttacgacaat
     1801 atgccgtcag acggtccgaa agtattctta ggcgaatacg cgtcatgggg caacacatat
     1861 tacaacgcac ttatcgaggc ggcatatatg acaggacttg aaaacaacgc acatgcaatc
     1921 ggtcttgtat gctatgcacc gcttttatgc aatgttgact acataaactg gcagcccgat
     1981 atgatttggt ttgacaatca cagagtatac ggcagtgcaa attactatgt acaaaaaatg
     2041 tttatgaact gcacaggcaa caatctgctt gacgtaaagc acgacggttt tgacaagcct
     2101 atcactcttg gcagtgacaa aatcagcggt aatattgaga ttgaagctga cagatgcagt
     2161 gcggaatttt acgatattaa aatcaccgac atcgcaacag gcaatgtaaa aacatatgaa
     2221 aatctatcat tcagcaacgg cggcaaagcc gttatagaca gcatagactc aaatcattac
     2281 aaagtcgaat ttaccgccaa acgcacagca ggcgacaaag gtttccgcct gttcttcggc
     2341 aagtcggacg ataaaaactt aattcaatgg tttatcggcg gttggcaaaa tcaagacaca
     2401 gaagtcaacg cacaagtaaa cggacgcggt tcgtgccttg accacaacat tttctccgta
     2461 atgacaggac aggaatacaa actttgcctt gaagtcaacg gcagaaatat tactacatat
     2521 ataaatggca aaaccgcaaa cactgcaatc gacaaacagc ctgttatgga ggaattgtac
     2581 tacaccgcaa gcagtgacga taacaacatt tatattaaag cggtcaatgt gcgtgacacc
     2641 gaaataacat ctgaaatatg cgttgaaggc gtaaacggca ttaacgcaaa cgtaacggag
     2701 ctttccggca attcgcttga tgatataaac gatttcgata atccgaaaaa ggtatcgccg
     2761 aaaacatcgg ctttaacagc cgtatcaaat acatttgaat acacattccc gccacagagc
     2821 gttacaatat tcactgtaca gaaataaaaa aatcataaag ttcctcaaac gtcgaaactt
     2881 tagattttat gccatttctg tcgcacatgt cgccagaaat gatttttaat gaggttagca
     2941 cctcaaactc ccaataacaa agtaaacaaa accataacgg ttttgtttac tatctaaatc
     3001 ggacgattac aatcgtccga tttttttgta atcaaatatt acaaagcgga ataaatataa
     3061 acaaaagggg gtatccgtat ggaaagcttt gaaactgctg caccgataat agttcttatc
     3121 tgttcactta ccgcactgtt attttcattc atcaaatttt actccgtaaa gcgaaaaagc
     3181 gaaggtacgg atatgatgaa aagcatcagc gtcaaaatac gcaaaggtgc gatggcatat
     3241 cttaaacgtc aatacaaaac ggtcggaata ttttttgcga ttatgttttt cattttgact
     3301 gtacttgcgt cgaaaagatt tctaagtccg tttgtgccgt ttgcattttt gacaggcgga
     3361 tttttttcgg gattgtcggg atatgtggga atgaatatcg ccacatacgc caacacacgc
     3421 accgcgaacg gtgttcgtga cggacttaac aagggtttaa aaatcgcgtt tgcgtcaggc
     3481 tctgtcatgg ggcttaccgt tgtcggttta ggacttttcg atataagtgt gtggtttatt
     3541 cttttgaaat tcgtgtttaa actaagcata aacgacgtta tgaccgctat gctcacattc
     3601 ggtatgggtg caagttcaat ggcactgttt gcacgcgtgg gtggcggaat attcacaaaa
     3661 gcggctgatg tcggagcgga tttagtcggt aaagtcgaaa tcggtatacc tgaggacgat
     3721 ccgcgtaatc ctgcttgcat tgccgataat gtcggcgaca atgtcggtga cgttgcagga
     3781 atgggtgctg atttgtatga atcgtatgtc ggctcgatta tttcgtgcgg agcgcttgcg
     3841 tcagcggcag gacttggctt taacggtgta cttgtgccga tgcttatcgc cgcaatcggc
     3901 ataattgcgt ctattatcgg cacgtttttc gtttcgacaa aggaaggtgc aacgcaaaaa
     3961 tcgcttttag gctcattaag acgcggtaca tacattgcgt caatcctgtc ggcagtcggc
     4021 tcggcatttc ttattttcac attacttccc gacaattcaa atgtattttg ggcggttatt
     4081 tcgggactta tcgcaggagt gatgatagga tatttcacag agtattacac gtctgactct
     4141 tacaagccta caaaaaacct tgcaaaatct tcaaatacag gctcggcgac aatcataata
     4201 gacggtatcg cactgggaat gtcgtcaacg gcgatacctg ttattataat cggaatttcg
     4261 gtaattataa gctactttac cgcaggaggt atggcaagct ttgaggacgg actttacgga
     4321 gtcggtctgt cggcagtcgg tatgctttca acactcggca taacgcttgc gacagatgca
     4381 tacggacctg ttgccgacaa cgcaggaggt attgcggaga tgtcggggct tcccgatata
     4441 gtgcgtgaaa gaacggacgc actcgattca ctcggcaaca cgaccgccgc aacaggcaaa
     4501 ggttttgcaa taggctcggc ggcactaacc gcgcttgcac tgatagtttc atacaccgat
     4561 aaaataaccg aaatgggaat tgaacttaat ctttccgtca ctaatcccgc agtgcttata
     4621 ggcttatttt caggggcaat gctgccgttt ttgttcagtg caatgactat gagagctgtc
     4681 ggacgtgcgg cacaaaaaat tgttgttgaa gtaagacgac agtttaagga gataaaagga
     4741 ttgattgacg gaaaagcaga ggctgattat gaaacgtgcg ttgacatatg cacaaaatct
     4801 tcgctgaaag agatgatttt ccccgccgca ctcggaatac ttgcaccgat aattgtcgga
     4861 ttggtgcttg ggtgcaacgg tgttgtcggt atgcttgcag gtgctttaat atccggtttt
     4921 gcacttgctg taatgatggc taattcgggc ggtgcttggg ataatgcgaa gaaatatatc
     4981 gaggcaggta atttcggtgg taaaggcagc gaaaatcata aagccgccgt tgtcggcgat
     5041 acggtcggtg atccgtttaa agatacttcc ggtccgtccg ttaatatatt aattaaactt
     5101 ctgtctatgg tttcaatcgt tttcgccgca cttatcgcca catacggtgg tatggggctg
     5161 ttttaggcat acaaaaaggc aagccacata agtgacttgc cttaaatttc ttattagcct
     5221 tgtgtattgt agctgtagtt tggagcctct ttagtgatat aaatatcatg agggtggctt
     5281 tcctttagac ctgcacctgt aattcttacg aacttaccgt tttcctttaa caactgaatt
     5341 gtcttagttc cgcagtaacc catacccgaa cgcagaccgc cgagtatttg gaatatagta
     5401 tcagaaacag ggcctttgta aggtacacga ccttcaacac cctccggaac aagtttcttt
     5461 gaacctgttt ggaagtatct gtccttactt ccgcattcca tagccgcaat agaacccata
     5521 cctctgtaaa ccttaaaacg tctgccttgg aatatttcaa attcgcccgg gctttcgtca
     5581 cagcctgcaa gcaagctgcc aagcataatt acgtcagcac ctgccgcaac agccttaaca
     5641 acatcgcccg aatacttaac accgccgtcg gcgataacag ggatattgta cttctttgca
     5701 acttggctta cgtcatatac agcagtaatc tgcggtacac cgatacctgc aacgacacgg
     5761 gttgtacaga ttgaacccgg accgataccg accttaacac agtctgcgcc tgcgttaata
     5821 agtgcctctg ccgcctcacc tgttgcgata tttcctgcga ttaagtccaa atcagggaat
     5881 gcctccttga tttccttaac cttatttatg atattcttac tgtgaccgtg tgcactgtca
     5941 agtacaacaa cgtcaacacc tgcatcatat acagccttaa ctctttccaa tgcgtcgttt
     6001 gtaacaccga ttgccgcacc gaccaaaagt ctgccgttct tatctcttgc agaattcgga
     6061 taacggatag ctttttcaat atcctttatt gtaataagtc cttttaagtg atttttctca
     6121 tcaactatcg gcaacttctc aatcttgtgc tttgaaagaa ttttctgtgc ctctgaaaga
     6181 gttgttccga caggagcagt tataagattt tcgcttgtca tagcgtcttt aattttcttt
     6241 gcaaaatctg tttcaaatct caagtcacgg tttgtaatta taccgataag cacgttattg
     6301 tcatcacaaa tcggaacacc tgaaatttta tatttaccca taagctcatc tgcgtctttt
     6361 agcgtatggt tttcagaaag actgaacgga ttggctataa cgccgttctc acttctcttt
     6421 actttatcaa cttccgcagc ctgctgttca atcgtcatat tcttatgaat tattccgatt
     6481 ccgccctctc gtgcgattgc aatagccatt gacgactctg taacagtatc cattgccgaa
     6541 ctcatcagcg gtatgttaag cgttatgttt ttagttagtt ttgttgttaa gtcaacttcg
     6601 ttaggtgtaa aatcagatgc ttgcggtaca agtaatacat catcaaatgt aagaccttcc
     6661 ttagaaaatt tttcttccat tcttataact cctctcatta gtattaattt tattaattat
     6721 aacaaatttt gcggtttttt tcaagacctt ttttacgtca ttttaaagtt tattataatt
     6781 gattaatata gtctgtaagg catattcgca ttgtggcaat atcactaatt ttgaaaactt
     6841 cgccctttaa tctcgatgaa cccgcaagtc ccttgatata ccacgcaata tgctttctcg
     6901 cctcttttat tccgcggctt tcgccctttt cttcaacaag catttcaata tgttccaacg
     6961 cttgtgcaag tctgtcctga ggagtcggtt caaagttcac tttgccgttt ttcagatatt
     7021 cgtttacctg tctgaatata aacggatttc cctcgcagcc tctgcctatc ataaccgcgt
     7081 cacaacccgt catatcaatc attcttttcg catcctcggc acattttata tcgccgttgc
     7141 cgattaccgg aattgaaacc gattctttta ctttctttat tatatcccaa tccgccgttc
     7201 cgctgtaaaa ctctgcccgc gttctgccgt gtatcgcaat cgccgccgct ccgccttgtt
     7261 ctataatctt tgcaacttca accgcattta tactgtcatc gtcccaacct ttgcggattt
     7321 ttaccgtcac cggcacaggc gacgcggaag aaactttatg cactatctcg cctatttttt
     7381 cgggcgtctt aagcaatgcg ctgccgtcac cgttgtttac gattttcggt gtgggacagc
     7441 ccatatttat atctattata tcaggtttta ccgccataac tttcggtatc acttccgcca
     7501 ttatatcagc gtcagagccg aatatttgta tcgcacaagg acgttcttcg tcatctatcc
     7561 gcattaaatc ggcggttttt ttatcgttgt agtacagtcc tttcgcactc accatttccg
     7621 aatacacaag tcctgcgccg tatcttcgac atatttttct gaaaggtaaa tcagttactc
     7681 ccgccatcgg tgcaagaaat acgttgtttt ctgtttcaaa atttcctatt ttcattgttt
     7741 cgccttttat ttttcccttt gtttttgatt atatccaaaa cagacaagcc tgtgttgtgt
     7801 ttttggcttg cctgctgttg tgttgccacc actccgtcac ttcgtgacac ctctcctcaa
     7861 ggagaggctt ttcggctccc atcgagggga gctggcggca acgccgtctg aggggtggca
     7921 aaaaaatatt atcttctttt tgagtattcc aatattttat gattatttga gaagaactgc
     7981 aaatttgcgt caaaatacaa atgtgaaact tcgcccttat tcatttcacc gttttcgatt
     8041 ttatttgcgc aatcccatgt tgtatcgaca atcatccatc tgccgtcaac gtatacttca
     8101 ttccatgcat gattttgttc gtcggttgca atacttgtat ctgtccacgc ggtatcatta
     8161 ccgacaccaa gtgcgtatcc cgacacgaca ttacacggta tatcaatact tctgcacagc
     8221 gacgccataa gcgttgcaaa accaagacac actgccttac ggctctttac tatatccgtt
     8281 gcgtaatacg gcggtgcctc gtcacttgcc aggctgtcaa cgtcataata catataagaa
     8341 catatccaat cgtgcaacgc aagcactttt tcatagtctg tcgttttatc cgccgtaagc
     8401 tgttcggcga tagagattat actgtcactg tttgattgta tagacgcggt gtatttaagt
     8461 gcctctttaa ttgacttatc cttttcatac atcgccttat tatgttcaaa cacaggtgac
     8521 tgttcgataa cccaaccgcc gttgccgtca cgcacaagtt ttacataatt atacacccag
     8581 ctttcaaact gtccgtaagg cttatcaccg gtatacacgt caacaccgac agatgagccg
     8641 ttcgttttcg gaattgatat tgttttattg aaactatttt cggaatttgc cgtaatcgaa
     8701 acctgttcaa gcgtttcacc gttttcatcg ataaatgccg caatcaaatt tgatttactt
     8761 cttgaaccga caattccgaa tatctgtatt ctgtcataat ccgcaatcat agaaatattt
     8821 tcgttcggat aaaacgaaag cataggactg ttctgtatat acgcattgac cgccacatct
     8881 gacggatcaa taggtaaatc ctcctgtttt atcattgcca taagctcgga cggtgtagga
     8941 atataatccg tcaccctcga aagccgttcg gacaatgccg aaactgactc agtaacctcc
     9001 ggacaatttt ctttaagata cggaacataa cttccgaaat tcaagaaaaa gtctgtctgc
     9061 gaattaatca cggctattcc tattgctaat atcaaaagta tggcaaaaaa tttctttatc
     9121 tttttcatat cataccacca taccaaaatc aaattttata taaaaaatgc tgcgacctaa
     9181 gtatattctt aagccgcagc atcttcctta ttggaggcgc cacccagatt tgaactgggg
     9241 aataaaggtt ttgcagacct ctgccttacc acttggctat ggcgccggat acaaataaca
     9301 aaagacgcat atttttgcgt cttttgtctt gtagtaaatg gagcggaaga cgagattcga
     9361 actcgcgaca ttcgccttgg caaggcgacg ct
//
LOCUS       DAWACS010000012         9168 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_872_length_9168_cov_2.633381, whole genome shotgun sequence.
ACCESSION   DAWACS010000012 DAWACS010000000
VERSION     DAWACS010000012.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9168)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9168)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9168
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_872_length_9168_cov_2.633381"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3416..3425
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6447..6461
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7270..7279
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2868 a         1555 c         2117 g         2593 t
ORIGIN      
        1 atgaacagat tgtgacaggc gacagtatag ctttaccgca agcggacggt attgtatgga
       61 atgaaacaga taatctcgga cttataaaca ccgaaacagg tgcgattaag cataataaaa
      121 ccgccgaaac agtaacgctt gtgcagtcat atgggggtgt agacagagag tatacattgt
      181 atgtgacggg cagtgaggaa gaaccttatt cgattaatat tgacgcttcg gacagaagta
      241 aggatgtttc ggacgatatg tggggattgt tcttcgagga tattaactct gccgcagacg
      301 gcggacttta tgcggaaatg gtgcaaaacc gctcttttga aaagagtgat gaaaaagaag
      361 gacatttata taattgggga ctgtattcaa tatataaaga gcctaacaga gaggacggca
      421 aatattatac cgccgaaaat atacaggtta aaacagataa accgatgaac gaaaataatc
      481 ctacatatat tacaattccg aaaggtgaaa tgatttgtaa tgacggtttt tatgacggaa
      541 ccgaatggga ttgcgataaa ggtacatctt tcagcggaat gaaatttaaa aatggcgaaa
      601 aatatgattt ttcggtatat gcacgcggcg agggtgaact gacggtgtcg ttgggaattg
      661 taatagatga acataaggct agttttgagg ttgaaccttt taatgaggct gctgccgtta
      721 taaaatgtaa ttcaaaagac gattggaaaa agtattctgt tacattcact gctgacgaag
      781 aacttgaaag tgacggcagc ggaattattg ttacaaatac aggcgacagt gaccttgatg
      841 tagatatggt atcgcttatg ccggaggata catacaaagg acacggcttg cgtaaggatt
      901 tgatggaaca gcttgaggca atgcatccta aattcttgcg ttttccggga ggttgtgccg
      961 ttgagggaca gacaatggat acggcttgga actggaaaga tacaatcggt gatgtgtcgg
     1021 agcgtaagga aatgataaac atatggaatc cgtctgcgac agagccgtat atgatgactt
     1081 acggacttgg gttttatgag tatttccaaa tgtgcgagga cttgggaatg gagcctgttc
     1141 cgattttgaa ttgcggtatt gcatgtcaag tccgcagcgg aagtgcaacg gacgaagaac
     1201 accttgttcc tatggacaaa ttacagccgt atattgacga tgcgcttgac cttatcgaat
     1261 ttgcaaacgg tactgatgaa agtaatgaat gggtgcaaaa gcgtattcaa atgggacata
     1321 aagagccgtt taatatgaag tacataggta tcggaaatga gcagtacggc gatatttact
     1381 ttgaaagata tgaagaattt gcaaagcaaa tacacgaaaa atatcccgat attaaccttg
     1441 taacaacgtc aggcactgcg tcaagcggaa gttcaaatga ccttgcatgg aattgggcga
     1501 atgaacatga ggaacttgcc gaccgtatgg acgagcatta ttatgaaaca gccgattggt
     1561 tcagacagca cgcttacaga tatgataatt acagacgtga tacaaataca aaggtatttt
     1621 tgggtgaata tgcgtcaaag ggtaatgctt ggtataacgc actgtcagag gcggcgttta
     1681 tgacaggtct tgaaagaaat gcggacgttg tgcgtatggc gagctacgcg cctatgtttg
     1741 cgaagtacgg caatacacag tggtcggcgg cggatatgat ttggtttaat aattcggatt
     1801 acgttcttac accgaattat tacgttcagt cactgttctc aaataatcaa ggcaattatt
     1861 ctttgccgac agaagttaag ctgaacggta tcgaaaaaga cgacgcactt caaggtggtg
     1921 ttgcagtcgg cagttgggga acacataatg aatttaaaga tataagatta tattcgggtg
     1981 atactatcgg tgtattgacg ccaagtgagt ctggagaata tgatgatgag gacgattata
     2041 acttagatga agaatatgat gaggacgatt ataatttaga agatcggggt tggaagattg
     2101 gcaaaggcga atggacaatg aataaagaag gcacacttgt ccaatcctct gatgaaacag
     2161 gtgcgatatg ttattttccg tatccggata acagacaata tactttgact ctgaaagcac
     2221 gcaaattgag cggcggcgag ggtttccaaa taggagttgc ggcagatgat gcattgaatt
     2281 attacagagt taatataggc ggttggggca atacaaccgc aaaggtacag cacattgtaa
     2341 acggtgtaag ttcttcttcc ggtaacgttg cggaacagtc atatgtcggt aacgtgcata
     2401 taaatgataa tgaatggtat gacgttacgg ttgaagtgac tgatgatgaa atcaaagcat
     2461 atttgaatga tgaatttata tgctcatata aaaagcctaa ggaatacggt cctgtgtatt
     2521 cgtcaagtgt gtatgataag gaaacaggtg acgttatcgt taaggttgtc aatacaatgg
     2581 acagtgacgt aaatatcgga atgaatgtat cgggcgaaac gataacgtca aatatcgcaa
     2641 agacaactgt tatgtcgggc gatacaaatt tggaaaacag tcttgataat aaaaatgcaa
     2701 tcgtaccgaa agaaatcgaa ttaacaaatg caagcaataa ctttacatat aatgcgcctg
     2761 ccgattcatt cagtattata agacttaaaa caggaaacgg tggaagtaag gcgtatattt
     2821 cgggatatga ggacggcacg ttcagaccgg ataatacaat aacccgtgct gaggcggcgg
     2881 caattatagc aagatgttcg gctgattttg acgaaaataa gatgtatgca agtgatttta
     2941 ctgatgtgtt gggtgatgaa tggtatgcaa attatgtcgg atatgccgct gaaaagggat
     3001 atatttcggg atatgaggac ggcacgttta aggctgacag aaatataaca cgcggagaat
     3061 tggcagttat tttgagtaaa tacggctcat ttgacggaga tggaatatgt acagaatttt
     3121 cagatgtgcc aaacgattat tatgcaacag gatatataaa gtcgttgtat gatgaaaata
     3181 tcgtaagcgg atatgaggac ggcacgttta agcctgataa ttcagtaact cgcgcggagg
     3241 cggttacaat gatgaataaa gtgcttggta atccgatagc ggaaaatgca gaaaatccgt
     3301 tcggtgatgt ttcaccaaat cattgggcat ataatcaaat tatgaccgca gtacagggaa
     3361 agtagcggac tgtctcatat ggtataacgt gggataacaa taaaatgcca cccccnnnnn
     3421 nnnnnctgtg ttgacagctc ccctcaatgg gagccaaaag cctgtccttg aggagaggtg
     3481 tcacgaagtg acggagaggt ggcagatgcg atataacgaa agtaggggac ggcacgtcga
     3541 tgtcccttgt aagataaccg taggggcgac cattggtcgc ccgttgaaga aaacacaaaa
     3601 agggcgaccg atgatcgccc ctacgaaagc cactctttaa aggagaggtg gcaaaaataa
     3661 aaggagaaac acaatgaaaa gagaagatat aaacatacgt gatccattca tattgccgta
     3721 tgagggcaaa tattacctat acggctcacg cggaagtgaa gtgtgggagg acaacgcgac
     3781 agggcttgac gtatacgtca gtgacgacct tgaaaattgg tctgaaccaa aagagatatt
     3841 tacgcgaacg gcagattttt gggcggacag acattattgg gcgcctgaag tttatgcgta
     3901 caacggcgcg ttctatatgt ttgcgtcatt caaatcggcg gacagatgcc gcggaactat
     3961 ggtattaaag gcagacaagc cggacggcaa atttacggta cacagtgagg gtacaattac
     4021 accgccggat tgggaatcgc ttgacggcac gttatacata agcaaaagcg gtaagccgta
     4081 tatgattttt tgccatgaat gggtacaggt aaatgacggt gaaatgtgtg cgattgaaat
     4141 gagtgcagat ttaaaaagag cagtcggtga accgattttg ttgtttaaag cgagtgaggc
     4201 gagctggatt gccgaggcac agaaaaacaa aggtatatac gttacggacg gtccgtttcc
     4261 gtacagatgt gctgacggta cattactttt gttatggtca agtatgggcg aagaaggata
     4321 tacagaggct attgcagtgt cggataatgg cgacatagac ggtaattggg tacaacgtga
     4381 cgaactgctg tttaaaaaag acggcggaca cggtatgatt ttcaaaacgt ttgatgacaa
     4441 attactgtta ttattacatt cgccgaacaa gcatccgctt gaacgtcctg cgtattttga
     4501 acttgaggat aaaaacggaa tgttgtatgt gaaatagggg aaaagtgatg aaaaaaacaa
     4561 tatctataat tataagtatt gcgatgtgtg caatgcttgc gtcctgcggc ggtgtgaaga
     4621 acgaagtatc tgatacagaa cagccgacgc tcatagatac aatgagcctt gaagaaaaag
     4681 tcggacaaat tctgtttgtg cgttgcgttg atgacgaaca aacagatgat ttaatgagta
     4741 taaaaccggg cggaatattg atgttcggac gtgactttga gggacttaca aaagacgaag
     4801 taaaagaaaa aatacagagt tatcaagata aaagcgacat accgcttata atcggtgcgg
     4861 acgaggaggg aggaacggtt gtaagagtaa gttcaaaccc taatcttgca cccgaaaaat
     4921 tcaagtcacc gcaagagatt tacaatgagg gcggaatgga cgcagtagtt gaaaatgcaa
     4981 cggaaaaatc ggaacttctt ttgagcttgg gagttaatat gaaccttgca cccgttgcgg
     5041 acgtttcaac aaatccgtct gattatatgt atgacagaag tcttggacaa aatgcggaag
     5101 ctacggctga tttcgtttca aagacggtaa cggcaatgaa taacgcagga attatgagcg
     5161 tattaaagca ttttcccgga tatggcggtg taggcggtga cagtcatcaa ggcactgttt
     5221 atgacgacaa aactgccgag agcatacgcg aaaacgactt gcttccgttt aaagccggaa
     5281 ttgaggcagg tgcaagcagt gtgcttgtgg cacataatac ggtcaatgca cttgacagtg
     5341 aaaaccccgc gtcactttcg ccgacaattc acaatatgct tagagatgag tgcggttttg
     5401 acggtgttat aatgacagac gacattgcta tgggtgcagt cgcagatatg gagaatgtgt
     5461 acgttaaagc ggtaaatgcg ggtaatgatt tacttataac gaccgattat caaacaggtt
     5521 ataatcagat tttaaacgcg gttaaatgcg gtgaaatcag tgaagagaca ctaaacagtg
     5581 cagtagagcg agtgcttaag atgaaagggc aaatataaaa caaaggctgt cgaaatcgac
     5641 agccttttat aatgtctgta atttatcagc ctttgattag gttttcgtaa tcgtcctcag
     5701 gcattggctt tgcacaatag aaaccttgaa ttgtatcgca atgcatttct ttcaacaaat
     5761 cgtattgact ttttgtttca ataccctctg ccgtaaccga catttgaagt gtatgtgcaa
     5821 gcgatattgc gctcaaaaca atagcctttg acttatcgtc tgtttcaagt ttatcgacaa
     5881 aacgtttgtc aagtttaacg atgtcacaag gcagaagatt aagcatatta agtgaagaat
     5941 atccgctgcc gaagtcatca agcaaaactt taattccgtg ctttttacat tgctttgtaa
     6001 gcgaaataag caagctttcg ttttctgcaa gaatactttc ggtaaattca acttcaatca
     6061 gatttagcgg tattccgtat ttttcaactg ttttgatatg atcctcaaca aagttttcgt
     6121 ccaataactg caagagtgat acgtttaccg atattggcga aacgtcatag cctaaatcga
     6181 gccattttct tattttggca caagtttctt caagtacata tttgtcaagc ttaacaatat
     6241 cgccgttctt ttcaaacagc ggtataaaca taccgggtga aattattgtt ccgtcaggct
     6301 tttgccaacg tacaagtgcc tctgcaccgt tgaatttgtt tgcgttaata ttgaatttag
     6361 gttggaaata aaccttaaat tctttgtttt taatagcatc ctcaaagccg ttttcaatcg
     6421 ttttctgcat cataatatca ttacgtnnnn nnnnnnnnnn ncttttgtgt tgttcttgca
     6481 cgattaagca ttgaatttat attcatattt ctgtcatcca caagataaat tccgacagat
     6541 accgtcatat tatatacacc gcctgccgga ctgtataatt taagcgagtt aaagaaatct
     6601 gcgcaagcgt tatctaacga cttagtattg tcgtatgtga ttaatgcgac aaagtggtcg
     6661 tcaaaaattc ttgaacacaa ttcttttggc tcaagtcgct tataaatggc agaccacaaa
     6721 aattttaaag ctctgtcgcc ttcgtcatca ccgaaaaagt cattgatata ttggaaattg
     6781 tttatatcaa ggttaattaa agcataattt tcatcgggat tgtctttcag tattttctca
     6841 gcctcaaaaa caaattttgt gtatgacata ccgcctgtaa gactgtcaac gtaagccatt
     6901 tcctcaagct gcttttgacc ggtggctttt gctttgattg ccgtatgcaa taggtagaaa
     6961 aatacgattg agcaaaatac gatgaacagg taacagcata taagaatttg agttgtgtac
     7021 tttgtcacaa cggtttttgg cacaacggta agtatgtacc agtcgtttat attaagcggt
     7081 gtataataaa tgtatttttc aatgccgtta agcattatta ttccgtctga tttaccttct
     7141 tttaaatcgg cttgcatttg ctgtatggca ctgtcatttt tgcttgacgc ttgactcatt
     7201 tcgtcaaaca gattgtcgaa tgtcaatccg aatgaaccgt catgagatga gccgacaatt
     7261 ctttttccgn nnnnnnnnng tgcatacaag aaatgtaaaa tcattatatg aatcgcaaaa
     7321 gaatttagtt tcttcgctgt taaaagactc ttttgtatta ggaaattgcg ataagtcgat
     7381 ttcgactccg tttatgtcgg taagcttgcc gtcaggtgtt acaattccta aaagatgctg
     7441 ttgattagcg atttggctat gctttaaaaa tgtaagaata tcgtcaaaag aggcataacc
     7501 caatttttct atataagatg ccgcattttc tatgattttt gcatctcttc cgagttcaag
     7561 actgaaaata gtctgatttt gatgtgaaat ttcagaaaga gtagaaatcg ttcgttgatt
     7621 tgccgcatag gtaatataac cggcatagct gattataaca atagcaatag tgacaagcga
     7681 gacaattaga agtcccaaac tttttgatat tgttttttta tacatatatt ctgatgcctc
     7741 ccctaaaaag aaaatatgaa taatcataat tatattatca taataggcag aacagtgcaa
     7801 taatttcaaa taaaaaaaca catataaatg tgtttttttg gggattttac actaatatat
     7861 atccgataat gagtcgatgt ttgtgcggtc aaacataatc gggtcgccga gtattatttc
     7921 gcttgcattg tccttgtcgg cggtgattgt cttttcgccg agtgaaccgg cggtaaatgt
     7981 ttcacctatg ctgcctgtta tttcattttc gtcaagggct ttaagcgtgt aggcggtaag
     8041 gtagccgaga tcggtcgtat tccatatgta caatctgtcg cacgttccgt cttttatata
     8101 ttcggataat tcactcggaa aaccaagtcc tataagattt acatccttgc cggattcttt
     8161 aagcgctttg gcggcggaac tcaagcctat tgtcgtagga acgataatag ttttaatagc
     8221 gtcattttgc aaaagatatt gtgtttccgt gtaagatttg gttgcatcat catcgccgta
     8281 agctatttcg ataagaggca tattgctgta tttttcggga ttttcttcta tttcgagctt
     8341 tagatagcgt atccattgat tttgattcgg tgcactgcct gtggaagtta aaatcgcaaa
     8401 accgccttca ccgtttgaaa cgtcatacgc actctttata aaatttcgtc cgagtacttc
     8461 cggatcggct tgttgaatat gtgtttttcg tgaaattgaa tttacgggtg agtcaaccga
     8521 tattacttca atgcctgaat tcagagcctc ggtaagtgaa gattcaagtg cgtcggcatc
     8581 gttggcggaa atggcaattc cgtccacgtt attgtccata agttcgttta taatttcaat
     8641 ttgtttgtcg gcagttgcgg aagtaggcgc tttgtatatc gcttcaatac ctatttcgtt
     8701 gcaggcactt tcaaaaccct caaaaacttt ttggctatac ggattttgta tatcttttcc
     8761 gacaaaagca tagacggttt catttgctga tttctccgaa ggagaaggag tcgattcgct
     8821 ctgcggaaca gaacacgcag taagcaatga aatgcttatc gccaatatag agggaattga
     8881 aattaacttt tttatatatt tcttcataag tgtttcctcc tctaagttat acattttata
     8941 ttagtaatat tatacaccat aaaaacataa aaataaatat aaatatcagt caaaaataac
     9001 caaaaaaaat cttttttaaa aaaataaaaa tttttttaaa aaaagtgttg acaaagtaaa
     9061 aaaggtctgc tataatagtc aatgttgttg gccgacacaa caggtcagcg caaatgggag
     9121 tttagctcag ctgggagagc gtctgcctta caagcaggat gtcacagg
//
LOCUS       DAWACS010000013         8986 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_891_length_8986_cov_2.655918, whole genome shotgun sequence.
ACCESSION   DAWACS010000013 DAWACS010000000
VERSION     DAWACS010000013.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8986)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8986)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8986
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_891_length_8986_cov_2.655918"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5438..5447
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2894 a         1579 c         2123 g         2380 t
ORIGIN      
        1 gtataggtgt tttgtgataa aataataatg tacagttaaa gatattaatt tacaatttgt
       61 ctgtgcggta aacagcacaa aaacacagaa aaaagtcttt acaacaagac ttatatggtg
      121 tataataaag acaaaacaaa ataaaaccaa aaaaaacaaa gaaaagaggg aaaacaaatg
      181 tataagtctg actttgaaat taagaaagaa ttatgcgaaa tcggaaggag aatttataat
      241 gacggttttg ttgccgcaaa tgacggtaac ttctcagtca aaattgatga caacacttac
      301 tatgttacac cgacaggcgt aagtaagggt ttcatgactc ctgaaatgat ttgtaaagtt
      361 gatggacaag gtaatctaaa agaggctaac ggcccttgga aaccttcatc agaattcaaa
      421 atgcacttga aggtttatca gcaaagaccg gacgtaaatg ccgttgttca tgctcatcct
      481 ccgattgcaa cttcatttgc tatcgcaggt attccacttg acaagctaat tatgccggaa
      541 gctatcatct tccttggtgc agtgccaatc gcacaatacg gtacaccgtc aacaatggaa
      601 attcctgaat cacttgaacc atatcttcaa gattatgatg caattctact tgcaaaccac
      661 ggtgcacttt cattcggttg tgacctaaac acagcattct tcagaatgga atcaacagaa
      721 ttttacgcaa agctactttt ctatgcaaga atgcttggag gtgaaaagga aatcccttgc
      781 ggtgaagtta agaaacttgt tgaacttaga aagcaattcg gcgttccggg taagcaccct
      841 atggaaaagc tttgtccggg ttgttatgaa ggtgacgata cttgctcaat gtcaccgaca
      901 gaggctaatg aaaagtacgg cgtagaatca tcagccgctt atcatggttt ccaaccgctt
      961 ccttcacagg tttgcccgac tccttcatca tcaaataagg atatggaaag ccttgttgca
     1021 gaagtaacaa agaaagttat ggaacaactt aataaataag taacggagaa acatcatgaa
     1081 aactattgat gaacttgtcg atgagttggc agtgcgcctt aacttcggac aaaccgttaa
     1141 acaaaaaaat gccgcaaaac ctgtttctgc tcctaaaccg acggaggtta aaccggaagt
     1201 gaaaaaagag tgtaaaaaac aatgtaagtg cggcggaggt tgcaaacatg aaagtcccga
     1261 aaagatgact attgctatgg cgaaagaact tgccgaggca gttgaaaaag cggcgacgat
     1321 tttgggagtt aaagtggttg ttgcaattcg tgacgagggt gcaaatcttg tacttcttca
     1381 cgcaatggat gatagttaca tagcaagtat tcaggcgtca caggataagg cttatacagc
     1441 cgtagcgctg aaaatgccta cacatatcgc acttgatgaa tcaagaggcg gctcgcttga
     1501 cggacttaca aacggaaacg gaatattgct gttgggcgga ggttatccgc ttagaacaga
     1561 caaaaaaata tacggcggaa tcggagtttc gggtggcaca aaagagcaag atacaactct
     1621 tgcaatggtt gccgacgcgt attttaaaac acgattcgct tgaggaggtt ggaaaagtta
     1681 atggtagatg aaaagcaagt aagcgaaatc gttaaaaacg ttattgccgg aatggatatt
     1741 tcatcattcg ataacaagcc tgcaagaaag cagcttggtg tattcgatac aatggaagag
     1801 gccattgcag cttgtaacaa agcttacacg acctttagac attataataa agaacaaagg
     1861 gaaaatatta taaaggaaat cagacgtctt actcatgaag aggctgaacc aatggctaag
     1921 ttggctgttg aggatactaa aatgggtaat gtttatcaca agattttaaa gcatcacctt
     1981 gttgccgata aaactcttgg tacatctgac cttgaaaccc gtgcactatc cggtgacaga
     2041 ggtcttacac ttgttgagat ggctccgttc ggaattatcg gtgcaatcac accgtcaaca
     2101 aacccaagct gtacagttat ttgtaacagt atctgtatgc ttgcaggcgg taacggtgtt
     2161 atattcaatc cgcacccgca cgcaaagaga atttcagcat atgctgttga ccttgtcaac
     2221 agagctattc ttgcggcagg cggtccggaa aatatcgtat gtacagtaaa agaaccgaca
     2281 agagaaactt ctgctaagat ggttaatgac ccatcagtaa gaatgcttgt tgcaacaggc
     2341 ggtccgggcg ttgttaaaat gctgctttca agcggaaaga aagctatcgg cgcaggcgca
     2401 ggtaaccctc ctgttgttgt tgatgatact gccgacattc caaaggcggc taaagatatt
     2461 atcgacggat gtacatttga taacaatctt ccttgtatcg ctgaaaaaga atgtttcgta
     2521 atgaaaaatg ttgcggacga gcttattcag aatatgttga agaacggtgc ttacctaatt
     2581 aatgcggcac aagttaaaca acttgaggat gttgtccttg tttggtcaaa gcctaaaaaa
     2641 gagggcgaac agccaaaacg tgttatcaat aaggattggg ttggacgtga cgcaaagaag
     2701 attcttgctc aaatcggtat caatgtcggt gacgatatta gatgtatcat ttgtgaaaca
     2761 gagttttcac aggcatttgt tcagacagaa cttatgatgc cgatacttcc tatcgtcaga
     2821 gtagatacat tcgacgaggc tgttgaaatg gctgttaagg ctgaacacgg caatcgtcac
     2881 agcgctcatc ttcattcaaa gaatgttgac catatgacac aatatgcaaa agcaatctgt
     2941 acaacaatat ttgttaagaa tgctccgtca tatgcaggta tcggctttaa cgctgaaggt
     3001 tggacaacct ttactattgc cggtccgaca ggtgagggtc tcacttctcc gagaagtttt
     3061 acacgtcaga gaagatgcgt actttcagac gcacttaata tcatataaaa aggagtgaaa
     3121 accttgaata taaacgaaaa tgatgtagca aagcttgtac aacaggtttt gcaagaaatg
     3181 acaagcggtg ctcctgcatc agctccaaaa gcacaagcag ctccgagcgg tgacattcca
     3241 aagactgcaa aagttgctat gatgacagaa ttaaagcact ttgaaattca ggaataccca
     3301 atccctgagt tgggtgatga cgatattctt gtaagagttg aaggctgcgg tgtttgcggt
     3361 acagatgctc acgaatataa gaatgaccca ttctcactta tccctgttgt tcttggacac
     3421 gagggtacgg gtgaaatcgt aaagatgggt aaaaacgtta agaaagacag cgcaggtaaa
     3481 cctttaaata tcggtgataa ggttgttact tgtatgattt tcaaagacga tcctgatatt
     3541 acaatgtttg accttaataa gcaaaatgta ggcggtgcag acgtttacgg acttcttcct
     3601 gatgataacg ttcacctaaa cggctggtac agtgactaca ttcttatcag aggcggttca
     3661 acagtattta acgtaagcga ccttgatctt gattcaagaa ttcttatcga gccatgtgcg
     3721 gttttggtac acgctgttga aagagcaaag acaactaaca ttttgagatt taactcaaga
     3781 gttgttgttc agggctgcgg acctatcggt cttatctgta tagcagttct acgcacaatg
     3841 ggtattgaaa atattgttgc agttgacggt aatgaacaaa gacttgcatt cgctaagaga
     3901 atgggtgccg gtgaaacagt taactttatg aactacaagg gtatcgataa ccttgcaaat
     3961 gctgtaaagg acgctttcgg cggacatctt gcagattttg cattccagtg tacaggttcg
     4021 cctgttgctc acagcaatat ctataagttc atcagaaacg gcggcggact ttgtgaactt
     4081 ggtttcttta tcaacggcgg tgacgctaca atcaatcctc actttgacct ttgctcaaag
     4141 gaaattacgc ttgtaggctc atgggtatat actcttagag attatgctac aacatttgat
     4201 ttcttaaaga gagcaatcgc aatcggattg cctattaagg aacttattac acacagattc
     4261 ccacttgaag aaatgaacga ggcacttgaa acaaaccttg ctcagaaggg attgaaaata
     4321 gcttacgtca gaaaagactt aatctaaacc tcggaagaaa ggctgaataa gtatgaatga
     4381 cgcaatagga attatagaaa tgttcggatt tgttactgcg ataacagcgg cagatgcggc
     4441 tgcaaaagct gcagatgtta aagttattgc aatcgactca aataagccgg caaacgccga
     4501 ttcggtagaa gtaccgctta ttatggctgt caaagttcaa ggcagtgtca gtgccgttac
     4561 tgcggcggtt gacgcggctg caagaacagc ggagagcata tccggactta taaataagca
     4621 cataattgca agaccaaccg acgattcgca aaaaatggcg aaccgaacaa gtgtcggcag
     4681 agataaggta ggtcatatac caaataacgc gtagcgtaaa tttaaggaaa ggaatgatat
     4741 aggagtgcag gcattaggat ttatcgaaac aagaagtctt accgcttcta ttgaagcttc
     4801 cgacgttatg accaaaaccg ctgacgtatg ggtagtcgga actgagaaaa tcggctcagg
     4861 tcttgtgaat gtagtgattc aaggcgatgt tgacgctgtt acggcggctg ttaaagccaa
     4921 aagagattgg agaggtttat gcagttaatg taattcctcg tccgcatgat gacattgcca
     4981 aaatcctgcc ttcgatttaa gtaggaggtt agcagatgga atattcatta ggtttggttg
     5041 aggtttcagc gttgggaaat gctattataa tgcttgacga tatgctgaaa gcggctgatg
     5101 ttgagtttgt tgcgacagag cgtaagcttg gaggcagact tgtaacaata gtcgtaagag
     5161 gcgaacttac ttccgtaaaa gcgtcagttg acgccggtgt ggcaagagct acaaaggacg
     5221 gatgtttgaa agcgtcacag gttatagcaa gacctcatca tgagatattt aagttcttgc
     5281 atcttgacga ggaggagaaa aagcctaact cggaaattgc gggaacacac gtcggagcga
     5341 taaaagaaac tccggttaag gctgaaaaac caaaaacagt tgagaaatca acagaaacgg
     5401 aaaagactgc gaaaacaact gccgaaaaga aaccggcnnn nnnnnnngtc gtccgaagaa
     5461 aacacaataa ttaagttcta atttgcacat tggtgctttt agataaataa attaagtaaa
     5521 aaagggcatt cgcccaagaa aaaataggag gaaatttaaa atggcagtag aagcattagg
     5581 tatggtagaa acaagaggtc ttgtagcagc aatcgaggca gcagatgcta tggttaaggc
     5641 tgcaaacgtt acactaatcg gtacagaaag aatcggttca ggtcttgtaa cagttatggt
     5701 aagaggtgac gttggtgcag atcaggctgc aacagacgca ggtgctgcag caggttcaag
     5761 acttggtgaa cttgtatcag ttcacgtaat tcctcgtcca cacagtgacg ttgaaaagat
     5821 tttacctgct gtttgattta gtaagtaaaa attcttatgt agaaatacat aaggcgaagt
     5881 atggacgtta agtccgattc cgacatggct gacgggaaaa tttaattttc ccgtcacggc
     5941 tatggggaat gaaacctcaa tttttaggag gaaaaaggaa tggacaacaa tttgcaagat
     6001 atgattgcta aggcagttgt agatattatt aagcaaaaag aagataataa aggaataaaa
     6061 gtcggcgtgt cggcaagaca tgttcattta tcacaggaac atttggaaat tcttttcggt
     6121 aagggacacg aacttactgt aaagaaaatg cttatgggtg atcagtttgc tgcagaagaa
     6181 tgtgtaacat tggtaagtcc gtcacttcgt acaatcgagg gtgttcgtgt acttggtcct
     6241 gtcagaaaac aatcacaagt tgaaatttca agaacagata cctttaaatt aaaggttagt
     6301 ccgcctgtac gtccttcagg cgagttaaag ggcagtgcgc cgatggcgtt ggttggtcca
     6361 aagggcagta tatttttaaa tgaaggctgt atcatagcca acagacacat acatatgact
     6421 cctgcagagg cagaaaagta tggaattaag gataatgaca tcgttgatgt tgaaatccaa
     6481 aactcaaaac cgacacgttt ctataacgtt caggtcagag ttagagaaga ctttaatacg
     6541 gaaatgcaca tcgatactga tgacgcaaat gcctgtgcaa taaagacagg cgacagagtt
     6601 gtgattttag aaaaataacc atattaggct ttttgtctaa taagaggatt gataataatg
     6661 attattggca gagtatatgg aagcgttgta tcaactcata aacttgaagg tttggtggga
     6721 tataagttta tgcttgtaca atgtattgaa aataaaaatc ttgtcgataa gttccttgta
     6781 gcagttgacg gagtcggcgc cggtatcggt gaggacgtta taattactac gggtagcagt
     6841 gcgagagttg caatcggtga tgcaaattca cctgttgacg caacgattgt cggtatactt
     6901 gacgagaaac aatgctgatt aaattcagta aataaaccca cataataact ctttggggag
     6961 gaagaagcaa aatgacattt gaagagcttc agaaaattgt atatgatgca ggtatagtcg
     7021 gtgccggtgg tgccggattc cctactcaca gaaaattttc agacaaagta aaacagatta
     7081 tagtgaatgc ggcagaatgt gaaccattga tgatggttga ccatcacatt ttggaacatc
     7141 accttcaggc acttgttgat acacttaatg tactaattga tacaatgggt gcagatgagg
     7201 caattatcgg tattaagggt aagaatatgc accttttgga tacaaaaatt gttgcatcac
     7261 ttgagggtac aaaggtaaga attaaggaaa tccctgacat ttatcctgcc ggtgacgaag
     7321 ttgtacttac atatgaaaca acaggcaaga ttattccgga gggagcaatc cctgtaatgg
     7381 tcggtgtaat ggttattaac gttgaaactg tttataacat tcactgtgca attacaaacg
     7441 gtcagcctgt tactcaaaag tatgtaacaa tcggcggtga cgttgatgag gatattacag
     7501 ttaaagttcc tgtcggaatg aagattaagg cgttgcttga ggcaacagga cacggtgacc
     7561 ttgacggtaa ggctgttatt aacggcggtc ctatgatggg tagacttgtt gatttggaaa
     7621 acgattctgt tactaagaca accaagggac ttcttatatt ccctgaaact cattctatca
     7681 ttcaacgtaa gagaatgcca atttcaatga cattaaagcg tgcgtctgca gcttgttgta
     7741 actgtacaat gtgtagtgat atgtgtccga gaaacttgct tggatataat atcaatgtgc
     7801 ataagacagt tcgtgcggca tcgcacagcg aagttacaga ttcagaaagc tttttgcagt
     7861 cggctctatg ctgcggttgc ggtgtctgta ccgttatcgg ttgtcagcaa atgcttgacc
     7921 cgcagaagat ttcaatggac gtaaaaggtg ctcttggaag aaagggtcta agacgtcaga
     7981 ataatcaagc gccacaacag gtaagacctg aaagagcgtc aagacttgta tcttcgtctg
     8041 ttctgattga cagactggga ataagaaagt atgttaaagc acacgttgaa agaaagtata
     8101 tagactttgc tccaaacgaa gtttatatcg aacttaaaca acacgttggt aagccggcaa
     8161 gtgctactgt aaaggtcggc gatacagtta aagttggtga cgttgttgct caaacagcat
     8221 acgaggacct tggtacaaca atgcacgcaa gcattgacgg caaggttaag gcagtcacag
     8281 acagatttgt tattattgaa agatagtggg tgagagatga tgaattcaat aggattagtt
     8341 gaagtaaaaa acgtatctaa gggtattgtt gtaactgatg aaatgctaaa gagtgcgggt
     8401 atcgtgctta taagttcagg ttcggtttgt ccgggtaaat ttgtcacaat cgtaggcgga
     8461 gaactttctg ctattcatgc ggctgttgac agagcggaac ttatcgcgga agatgcactt
     8521 attgataaat ttgtaatagg taatcttggt gataacgtat ttgaggcaat ttgcggaaca
     8581 gccgatgtta aggaaaagaa agcattcggt attgttgaaa catttactgc cgcaagtgca
     8641 attcaagcag ccgatgctgc tgttaaagcc ggtgacatcg aacttatcga agttcgtgtg
     8701 gctcgtggtt tgggcggtaa atgctttgtt tcaatgacag gtgatgttgc agacgttaaa
     8761 gcaggcgttg aggcaggagc aagaattgcg actgaacaag gcgtgcttat caatacagaa
     8821 gttattgcta atccacatcc tgaattgtgg gaagctgtac tttaatttga ttttacggct
     8881 taaaaaggta ctgcgtatgc agtacctttt ttacgtctaa atcggacaaa ttcgattgtg
     8941 tagcgtaata tttatataat catatttata tgcaaatgta tagtaa
//
LOCUS       DAWACS010000014         8858 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_910_length_8858_cov_2.841531, whole genome shotgun sequence.
ACCESSION   DAWACS010000014 DAWACS010000000
VERSION     DAWACS010000014.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8858)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8858)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8858
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_910_length_8858_cov_2.841531"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2704..2713
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3996..4005
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6041..6050
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2765 a         1844 c         1678 g         2541 t
ORIGIN      
        1 tattttttgt tttccaacac ttttttccga aaaacattca aggtttgata aaaccgaacc
       61 tgttattttt tcgacttgaa taactgcctc cggattagga ttttgcacca tatacataat
      121 aaacacctta tttttttata ttagacggta aaacggcttg cttttaaagc atacaaaaaa
      181 cagacaattt ggaactagtt ttattcacaa gatataacta ttttaatcaa tgtgatattt
      241 gcatttttta caaatttctt tgaactccgc ttttctttta tattcatcag gcaaggacaa
      301 aatttttata tacccatcga tacaatcaca attttctata cattcataat tatgtatatt
      361 catttctacc aacggacaaa aatacaaatc atcattcatt cctgtctaat acctccttaa
      421 ttttgatttt cgtatcacta tatacaaaat aaatataatg tttaatctta tttctttata
      481 taattaaatt acacaacagc cgctatccaa tttaacgaac aaaaaaccgc ttaaacatac
      541 gtttaagcgg tttcacttgg tgagccatcg gagattcgaa ccccggacaa cttgattaaa
      601 agtcaagtgc tctgccgact gagctaatgg ccctggcagg gatagcagga ctcgaaccta
      661 cgcatgccag aatcaaaatc tggtgcctta ccgacttggc tatatccctg tatattaaaa
      721 ttaaaatggg gtgggtagtg ggattcgaac ccacgacatt cagtgccaca aactgacgct
      781 ctaaccagct gaactatacc caccatatga tggcggtagt tatctacgca cactaaactc
      841 tttttgaatt ccaaaaagaa attggcgtgt ctggagggat tcgaaccccc ggcccattgc
      901 ttagaaggca attgctctat ccaactgagc tacagacaca tatattggag cgggtgatgg
      961 gaatcgaacc cacgcgacca gcttggaagg ctggaattct accattgaac tacacccgca
     1021 taattcaatc tgtctttcag tgagttctct actcaccaac tgacttgatt aattataaca
     1081 gaccaaaaag catttgtcaa tacttttttc aaaaaaattt gtcattttta tgtaccggga
     1141 cagatttctc tatcccgaca catttttact gattttatac acataatcag tcattttcgc
     1201 cacatcattt tttgaattaa aaactccgaa cgacgctctg accgctcccc tatccgatgt
     1261 acctatcgct ctgtgtgcga gcggtgcaca atgataaccc gctcttaccg ccgctttgcc
     1321 cttaagcatt tcaaaaactt cttcacttcc gagatttgcc acattaaacg cgacagtcgc
     1381 aacactgtta tcattcccgt ataccgtaac attatccata ttcatcaaat tttctttaaa
     1441 tttttcggaa agcatttttt catacttacc tattacatca acgccgtatt tcataacata
     1501 cttaacccct gcccccaaag ccttaattgc aggtgtattc atcgtaccgc tatggaactt
     1561 atcgggcata aattccggct gggaaacgct ctccgaatta cttcccgttc cgcccgtcac
     1621 aagcggtgca agttcttccg gacttttcac atacaatccg cctgttccaa gcggtcccat
     1681 aagaccttta tgtcccggaa aagctatcat atccgcattc attttttccg catcaatatt
     1741 tatgctccct gccgtttgtg caacgtccag caaaaacggt attttatgct ttttcgctat
     1801 tcttcctatc gtatacaccg gctgaattgt tccgcacacg tttgacgcgt gggtacagac
     1861 aatcagcttt gtatctttcc tcacagcctt ttcaacatct tccgcgtgta catatccgct
     1921 cttatccgct tttacaacgg tgtaattacc gggcaaacaa gcaggtctta aaacgctgtt
     1981 atgatccatc tccgtaacga caatatgtcc tccgtttgac attgtgccga gaatggcagt
     2041 gttcagagca tacgtcgcat tttgcgtaaa aacaatattt tcggcttttt ttatattaaa
     2101 aagctgtgca atcaaatctt gtgtatccac aatagcccgc actgagtcca tcctcatctt
     2161 atgtcctccc cgtcctgcgt ttccgcccga aaacaaggta ctcttaaaaa gcgtcgcata
     2221 caccgacaaa ggctttttca aagtcgttgc cgcgttatca agatacaaca tcataaacac
     2281 gctccccgtt tactgttctt tctatataaa tccgtctaat cggaatctca ttatctccgg
     2341 ctatctttct gatttcatta agtgcacggt catcaacacg cacggaatag gaacagccac
     2401 cttgatttaa agcggacggc gtatgcacaa catatgccgg aaaaccgata attttttctg
     2461 cagctttttt aagtcttgtc gcagtagtga ccgatccaat caatatatac ataaatttca
     2521 ctcccctcaa atattataaa aaggcaagcg agaaattctt gcctccttac tatataatat
     2581 tcaatcgacg atagtttgtg agtaaacaaa aaagactgcc gacaatttgt cgacagcctc
     2641 ggactttaga caaaaccata atggttttgt ctactttatt tttgggagtt tgaggtgcaa
     2701 cctnnnnnnn nnngaggaac tttatgattt tttcaaaaaa atactcaaaa attacatcgc
     2761 agatgtgatt tttggagctg gtgacatttt gtcaccaggc tcactttatt agtatacgtc
     2821 aaacacactt cgcggtttat tgtgatacga cacactaagc accagtccta ttgcaatcat
     2881 attcacaaca agtgacgtac cgccgtagct tacaaacggc aacggaatac ccgtaacagg
     2941 cataagtccg atacacatac cgatattttc aaatgcgtgg aacaacatca ttgcaccgac
     3001 accgacacaa atatatcttc cgaaagcatt atcggctttt tgagcggttt taaagcattt
     3061 ataaattatg aagaacagca ccgccgtaac aagcaatgca ccgataaaac caaattcctc
     3121 acttattaca ctgaatataa agtcggtata tttagtaggc aaataaccca tttggttttg
     3181 cgttccctgc aaatatcctt taccccaaag ctgacccgaa ccgaccgcaa ttttagattg
     3241 aattacgtta tagcctctgc cccttgggtc aaggtttgga ttgaaaaaca cctgaatacg
     3301 tttctgctga tattccgaaa gtacatactt atatataaaa ggcaatgacg caacacctgc
     3361 cacaccaatc ggcaatatat atttatatga aagttttgca acaaacataa gacatataaa
     3421 cataaacaaa aataccaaca cactgcccat atcaggctgt aacaaaatca aacctacaag
     3481 cacgccgata tgaagtaaca atcccaaaat cgtaagcggt ttatttatat cgtcatgcac
     3541 ttttgataag tgacacgaaa aagttactat aaagcaaatc tttgcgattt ccgaaggctg
     3601 aataccgatt gcaccgaatc taatccagct cctcgcaccc cagtcgccgc tgacaccaaa
     3661 caccaatacc aaaagcagta tcgcgacggc gaatatataa atcggtttga cgagattttt
     3721 aagctgttcg taatcgaaaa aacacgttac aagcattgcc gcacaaccta taaccattgc
     3781 acccgactgc actattacat tcgtattcga ctgcaatgtt cgcgtagccg aataaatcat
     3841 cactattcca aacaccgaca aacagattgt cgctataaac aaaacccagt caaactccat
     3901 aagtatgacg gatttttttg aaacactttt tattttcaaa aatatcactc ctattcagat
     3961 tacagacaaa acgattttat cacaattatt tatatnnnnn nnnnnattaa aaatcgttat
     4021 ttaatcgtca ttttcacggt aaaggaatat ttaatttttg caataattca ttcgcacggc
     4081 gaatttgttt tttacgatat tccgcaatgt aatgcgtatc agaaccgacg cttacgggaa
     4141 ttccgctttc tttgattatc tgctgtttct tttcgttggt aagaaaatca taataatata
     4201 tgtaatttcc cgaagtattc agttcccaaa aaatatcgtt gtcacgtaaa atttttatta
     4261 tatcaagtcc gtatctttca ccgagtttga aaatatcggt atgtgcaaga cctgctttgc
     4321 atttaaacag ctttcgccat tccaaaaatt cataaaaatc tattgccctt gtatcgtcaa
     4381 ggcattcaaa aagtacataa tcaaaatttt ccgttgcacg cggaagcgat tccggcggtg
     4441 tcggacgtgt accgatttca agaccgcaaa gaacttttat tttatcggca tattttattt
     4501 tacaatgctg aatatattca taatatatag gaagattttc accgattgag aattgatggt
     4561 cggttatacc gataacgtca acaccgtgtc tgattgcatt ttcaattatt tcttcgggcg
     4621 tgttcgagcc gtcatagctg aattttgtat gattgtgtaa atcaacggta tacatcggaa
     4681 aacctccttt ttctcatttg catatattat aacacacact tataaaaaat tcaattatta
     4741 ttaaaattta atttatttac aaacaataat atttatgata aaataacaat aataaggaaa
     4801 gaatacgacg aggagtgatt aaaaatggat tttttagaat tggtaaaaaa cagattttcg
     4861 ctcagaaaat tttctgataa aaaagtagag aaagaaaaga tagattatat tcttgcggca
     4921 atgcaggcag cgcctaccgc cgtaaatttt cagccacaga gaatacttgt acttaccgac
     4981 gaaaaagaac ttgaaaaggt gagcaaatgc acgaaatttg catttaatcc gccgcttaat
     5041 ttccttgtat gttatgacaa agaaacatct tggacgcgtg gaaatgacgg acacgatgag
     5101 ggtgaaatcg acgcggctat cgttgcaacg catatgatgt tggcggcaaa aagcgtaggg
     5161 ctcggaacga cttgggtagg ctcatttaat ccgaaggacg taaaagagca attcaatttg
     5221 cctgaaaatt atgttcccgt tgcattttta ccgacaggtt atccgtcaga cgacgcagag
     5281 cctgcggcgg ctcattcaag cagaaaagat ttaaacgaaa ctgttttcta caacagtttt
     5341 tctcaaaaat aataggcaga aaggggtatg tattatgaaa atttgtgtat tcggagcgtc
     5401 aagcagcaca atagataaat cttatgtcag ccaagtcgaa gaactcggac gtaaaattgc
     5461 tgacagaggt cacggacttg tttacggcgc aggtgcatcc gggcttatgg gtgcggttgc
     5521 acgcggtgtt tatgaaaaga aaggcgaaat tgtcggtgtt gtaccgaatt tctttgatga
     5581 tgaagatatg ggtgttgacg gccgtatttt ccaagactgt acggaactta tcagaacgga
     5641 cacaatgcgt gaaagaaaac gtataatgga agaaagagcg gacgcttttg ttattgttcc
     5701 cggcggaata ggcacttttg aggaattttt tgaagtgttt actctaaaac agcttgaaag
     5761 acataacaaa gctatcgcca tacttaacgt aaacggatat tacaacgcaa tgattgatat
     5821 gcttgaagtt gcggtcaagg aaaagttttt gagagccggc tgtaaacttc tttacaaagt
     5881 tttcgatgac gtggacgaaa cacttgacta cattgagtca tacaaagcac cgaaaattaa
     5941 cgtatttgaa atgaaagacg taaaaaatta atctaaaaaa caccgacaca tcggtgtttt
     6001 tttgtcgatt tataaaaaaa atatctaaac aatgatatat nnnnnnnnnn tttatttatg
     6061 gtataattaa tatacagaca tatggcggca gacgccgtcg ctactatttt tataacttta
     6121 ggaggagaat ccaatggcaa aaaagtattg ttaccttttt acagagggta atgcaactat
     6181 gagagagttg cttggcggta aaggtgctaa ccttgctgaa atgacaaata tcggtcttcc
     6241 ggtaccgcag ggtttcacaa ttacaactga ggcttgtact caatattacg aagacggtcg
     6301 tacaatcaat cctgatatta tggctgaaat caacgagtac atcggcaaga tggaagaaat
     6361 cacaggtaag aagttcggcg ataaggaaaa cccacttctt gtttctgttc gtagtggtgc
     6421 aagagcatca atgccgggta tgatggatac aatcttgaac cttggtctta acgaagacgt
     6481 tgttgaagtt atcgctaaga agtcaggtaa cgcaagatgg gcttgggact gttacagaag
     6541 atttattcag atgtattcag acgtagttat ggaagtcggt aaaaagtatt ttgaagaact
     6601 tatcgacaag atgaaagaag aaaagggagt tacttttgac gttgagctta cagctgacga
     6661 cctaaaggaa cttgcatcac aatttaaggc tgaatataag gaaaagattg gcgaagactt
     6721 cccatcagat cctaaagaac agcttatggg tgctgttaaa gccgtattcc gttcatggga
     6781 taacccaaga gctaacgtat accgtcgtga taacgacatt ccttattcat ggggtacagc
     6841 cgtaaacgta cagtcaatgg cattcggtaa catgggtgat gactgcggta caggtgttgc
     6901 atttactcgt gaccctgcta caggtgaaaa gaagcttatg ggtgaattcc ttataaatgc
     6961 tcaaggtgaa gacgttgttg ccggcgttcg tacaccaatg ccaatcgcaa agatggaaga
     7021 agaattccct gaggcatttg ctcagttcaa ggatgtttgc aaacttcttg aagatcacta
     7081 cagagatatg caggatatgg aattcactgt tgaaaataaa aagctttata tgctacaaac
     7141 aagaaacggt aagagaactg ctcaagctgc tttgaagatt gcttgtgacc ttgttgacga
     7201 aggtatgaga acagaaaaag aagctgttgc aatgatcgac ccaagaaacc ttgacacact
     7261 acttcaccca caattcgacg ttgccgctct aaaggctgct acaccgatgg gtaaagctct
     7321 tggtgcatct ccgggtgccg cagcaggtaa gattgtattc tcagctgacg acgctaagga
     7381 atgggctgca agaggcgaaa aggttgttct tgtaagactt gaaacttctc ctgaagatat
     7441 cgaaggtatg aaagccgctc aaggtatcct tacagttcgc ggcggtatga catctcacgc
     7501 agccgttgtt gcccgtggta tgggtacatg ctgtgtatca ggttgcggcg acatcgcaat
     7561 ggacgaagaa aacaagaagt ttacacttaa cggtaaagaa tatcacgaag gtgatacaat
     7621 ttcacttgac ggttcaacag gtaatatcta tgacggtatt atcccaacag ttgacgcatc
     7681 aatcgcaggt gaattcggca gaattatgtc atgggctgat aagtacagaa cactaaaagt
     7741 tcgtacaaac gcagatactc ctgctgacgc taagaaagct aaggagcttg gtgcagaagg
     7801 tatcggtctt tgccgtacag agcatatgtt ctttgaaggc aacagaattg acgcattcag
     7861 agaaatgatt tgtgccgata cagttgaaga aagagaagca gctcttaata agattcttcc
     7921 tatgcaacaa ggtgacttcg agcaacttta cgaggctctt gaaggtaacc ctgtaacaat
     7981 cagattcttg gacccacctc tacacgagtt cgttcctaca gaagaagaag atattgaatt
     8041 gcttgcaaag gctcaaggca agagcgttga ggctattaag gatattatca acggtttaca
     8101 cgaagctaac cctatgatgg gtcacagagg atgccgtctt gcagtaacat atcctgaaat
     8161 tgcaaaaatg cagacaaagg ctgttattcg tgcagctatc aacgttcaaa aggctcacgc
     8221 tgattggaca atcgttcctg aaattatgat tccgcttatc ggcgacatca aggaattgaa
     8281 gtacgttaag aaagtcgttg tagaaacagc tgacgctgaa atcgctgctg caggtatcga
     8341 acttaagtac gaagttggta caatgatcga aatccctcgt gcggctctaa cagctgacga
     8401 aatcgctaac gaagctgact tcttctgttt cggtacaaac gaccttactc aacttacatt
     8461 cggtttctct cgtgacgacg caggtaagtt ccttgacgct tactatgatg ctaaaatctt
     8521 tgaaaacgat ccgttcgcta agattgacca aaagggtgtc ggtaagctta tggaaacagc
     8581 tattaagctt ggtaaaccgg ttaatccaaa acttcacatc ggtatctgcg gtgaacacgg
     8641 cggcgaccca tcatcagttg agttctgcca caagattggt cttgactatg tttcatgctc
     8701 accattccgt gtgccaatcg caagacttgc agctgctcaa gctaagatta acgaagaaaa
     8761 ctaattcatt atcaaatatg ataatcagac aggcaaaacc tttcggtttt gcctgttttt
     8821 ttgtttaaaa ttttctcctc cacttaattt atttcaaa
//
LOCUS       DAWACS010000015         8654 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_941_length_8654_cov_2.715665, whole genome shotgun sequence.
ACCESSION   DAWACS010000015 DAWACS010000000
VERSION     DAWACS010000015.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8654)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8654)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8654
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_941_length_8654_cov_2.715665"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    905..914
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1407..1416
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7932..7941
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2800 a         1460 c         1962 g         2402 t
ORIGIN      
        1 tcctgcaccg taaatatgat ctgccaattc tgtatcatcg tttgtataat cggctgatgc
       61 ttttagtttg tcgattataa gttcactctt gattgatgca ccgttgaact taactttatc
      121 ctttacagga tatgacgatg cactgcttgc cgaaacagtc ggtgcatatg cagtaatgta
      181 aatcttaccg ccttcgtcac ggtcgataaa tgcgtttgtg atgtaagcgt aaggaattac
      241 ggcagtctgt tcaagtgttc cgaaaacggc tgtgttaaat gcgtcaagat agaatgtacc
      301 cgaaacgcct gttttaagag ttttgccgtc tttgtcattt atgtatgcct cacacttgct
      361 gccgatttta tatgatttac cgccgattgt cattttatcg ccgtttgaac cgattgaagt
      421 gattgaaccc ttaacaggat tatttgtaac aagaagtgtg tagtacgaac cgtcaaggct
      481 ctttgtataa agaattacat cgtttgcgga aattgatgta acaggaattt ccgaaccgtt
      541 ctttgtgatt gtgtatgtat aatcagacgc ttgagggtca aggattaagt agttacctgt
      601 tacgagcttg tctgttatac ggtaatctgt tgttgtagga gtggcataag caactattgt
      661 gtcatagttg tttatttgaa tcatatctat tgtgccgtcg tcaccgttgt ctgtaagttt
      721 aacgtcaccg tagattgtgt agtctgtgtc aggagtaagc cattgttcaa gtgcctcttc
      781 gcgtgagaat gtttcttcct gctttgttgt cggatttgta agagttacgg tttcgttctt
      841 tgcaacaagt ttgccgttgt atcttactgt aagttcatct tcttttagtt ttacagtctt
      901 tagcnnnnnn nnnntagtat ttaagtgagt tgccggaaaa actgtttaaa tcttcataat
      961 ctaatttgat ttcagaattt tttgttgttt cgtcgtcaat gatgataagc tttctgtcgt
     1021 ccttgtctgt caactgttca tagtaaacgg tgatagtatt gccaagatat ttgcttatat
     1081 ctgtaacgtt tgaagtgaat tccgagaagt cgatttttac aagatcactg ctgtcgttcg
     1141 gcaagatagc catttcactt tcgttaagat cttgcttgca gtcctctgtc aggtaatctt
     1201 caacacctac aagagtacct tttagctttt taacttttag gtagtcctgc ataagagttt
     1261 tctctgttgc aacccagttg taaccgttgt tttcgtatat aggtatttca agtgcgttgt
     1321 aaagtacctg tgcaatcata cctcttgttg caccttcact gtaacctgtt gaattaactt
     1381 tctttgtaag gtcaagagtg tttgccnnnn nnnnnnaact gaacataacc taatgtacaa
     1441 actaccattt taagagcttg ttcataagtt acaggagcgt ccggagcaaa agtaccgtcg
     1501 cccatacctg cgattatgcc aagattgtag gcagtttgaa tatagttctt tgcccagtgt
     1561 gaagcgtcaa catcagtaaa gtctgatgag tcgtatgcaa ggtctttgta accaagtata
     1621 aatacgataa gctttgtgaa ttcagctctt gtgatttgac cgtcaggctt gaaagtaccg
     1681 tcttcgtatc cgtcaataac tttaagcttt gttaaagttg taatagcgtc tttgtaagta
     1741 ttatcgtcac ctacgtcgct gaatgcagca aaaaccgatg tttgacatat gacggaaagt
     1801 gtcataataa gtgatgtcag aattgctgtt agttttttca tgagttattc atcctttcgt
     1861 tttgaattta gtgtagtaat attcagatac tgatatttta tcattctttt atatataagt
     1921 caattaaaat aatatatttc cgaattgtga attttaataa aattgtgtaa aaaaatttat
     1981 ttaacagtgc aataataaga ggaaagtgct acaatatcta ttgactgaca tttttaatat
     2041 tagttatata taaatatgaa gaaaaacgtg gaggaacatt tatggataat aaaaagaaaa
     2101 accgttcgcc gctgaaaaat ccgttgttga tatttttggt gatttcggtt attgcgacag
     2161 ttattttaaa tatggtaatg ctgtcgttgc agacaccgaa gaaacaggaa atatcgtaca
     2221 gtgaattttt gactatgctt gatgaaaata aggttgacga ggttattttg cagtcggagc
     2281 agatagtcat atatgaaaag tatgacgaat cacagccgat aacaacgcct aaaacaaccg
     2341 agtttatgaa gatgatgggc atagatacag acgcggttat agaacaggca aaggaaaaca
     2401 gcagaaatgt gtattatacg ggatatatcc ctgacgacag acttcttgcg gatttggaca
     2461 gtcacggtgt gaagtattcc acaccgatac agcataacag tcctgtgctt gatttcttcc
     2521 ttacatggat attgccgttg gtagttattt atttactgtt ctttattttg acacgttcaa
     2581 tgtcgaagaa aatcggcggt ggcggcggaa ttatgggaat aggtcagtcc aacgcaaaga
     2641 tgtataacgt agaaaatgcg acaggcgtaa cgtttgcgga cgttgccggt caggaagagg
     2701 ctaaagaatc gcttgacgaa atagtcgatt atcttcataa tccgtcaaaa tatcttgcaa
     2761 tcggtgcaaa acagcctaag ggtgcgcttt tggtcggacc gccgggaaca ggtaaaacac
     2821 ttcttgcaaa ggcggttgcc ggtgaggcga aagtgccgtt tttctcgctt tccggttcgg
     2881 aatttgtgga aatgtttgtc ggcgtcggtg cgtcaagagt tcgtgacttg tttaagcaag
     2941 catcggcaaa agcaccttgt attatattta tagacgaaat tgacgcaatc ggtaaatcga
     3001 gagataatca gattagttca aatgatgaac gtgaacaaac tttaaatcag ctgttaagcg
     3061 agatggacgg atttgattcg tcaaagggac ttgtaatttt agcggcaacc aaccgtccgg
     3121 aagtgcttga taaggcactt ttaagaccgg gtagatttga cagaagaatt attgttgaaa
     3181 agcctgattt aaaaggcaga gaagatattt tgcgtgtgca tataaagcac gttaaaacag
     3241 aacctgatat taatcttcac gaaatggcac ttgcaacaag cggtgcggca ggtgcagacc
     3301 ttgcgaatat ggtaaatgag gcggctttaa gagcggtaag atgtaacaga aagaccgtaa
     3361 gccaagagga tcttatggag gctgttgaag tcataatcgc gggtaaagaa aagaaagaca
     3421 gaattttgtc cgaaaaggaa aagagaattg tttcgttcca cgaagtcggt cacgcacttg
     3481 caacagcggt acagaaacat actcagcctg ttcacaaaat cacaatcgtt ccgagaacta
     3541 tgggttcgct tggttataca atgcaaatgc ccgaagagga agaacgttat ttgatgtcga
     3601 aagacgaaat agttgaccag attacagtgt tccttgcagg ccgtgcggcg gaagaacttg
     3661 tattcaacgt gcagacaaca ggtgcgtcaa atgatattga acgtgcaacg tcacttgcga
     3721 gaaatatgat tacgcaatat ggtatgtcgg aaaaattcgg tatggccgga cttgaaagca
     3781 tacagaataa atatcttgac ggcagaaatg tttcaaactg ttcggaggaa acaaagacag
     3841 atattgataa ggaagttgta cgcgtattga aagagtgtca cgaaaaggca tttaatatat
     3901 tgaaagaaaa tcgtgacgca cttgatgaaa tcgcagaatt tcttatcaat aaggaaacta
     3961 ttacgggcga tcagtttatg gagatattca accgttttaa aagcggcaga gaaaattctg
     4021 acagtgatgc agaagaaagt gccgcagtta atgaagaaaa tgataaagaa gaataacggt
     4081 acgttcaaaa agtgctttgc tcaaaggctt agggaactga gaaaagaaaa cggcataacg
     4141 caggcgggac ttgcggagag aatgaatgtt tcaagaacgt gtatcgcgaa ttgggaaagc
     4201 ggcaagcgta tccccgaagt ggcggcgata acgtatcttg ccaagctttt taaagttcct
     4261 gttgactacc tttgcggcag gacggacgaa cggtacagag tgaaattgct tgataatatc
     4321 gacttcgact taacaaaact aaacggcaaa ggcatcgaaa tgctttgcga atattacgat
     4381 tatcttgtca gcagtaaaaa atatagagca aaatagtaaa aaatgcttga catttgctga
     4441 aaaaagatat ataattacaa agcttgcggt aaacgtaagc aatccttgcc cacacgggat
     4501 atgtgtggga caaaagtcca taaggaggtg cagaaaatgg ctgaaaaaat actaaacagc
     4561 tatgaaacaa tttttatcat tgacgcaaca cttgatgagg aaacggttac tgctctaaag
     4621 gataagttta caacacttat cgaacagaac ggtgaacttg aatctgttga cgaatggggc
     4681 aagagaagac ttgcttacga aattaatgat agaacagaag gtttctacta tctcgtaaac
     4741 ttcaaggctg acagcgagtt ccctaaagaa cttgaaagac agtacaagat tacagaaggt
     4801 atcctaagaa ctattgttat cagaaaagac gaagaatagt cttttgtgaa gaaagcggtg
     4861 taagtatgaa caaagttata ttaatgggac gtcttacacg cgacgtcgaa atgcgtcaga
     4921 ctccgaacgg tgtttcactt gcgagatttt caatcgcggt aaacagacgt ttcgccggta
     4981 aagatgctca gcagcaggct gatttcatca actgcgttgc atggcgtcag acaggtgaat
     5041 ttatcgcaag atatttccaa aagggcagta tgattgcggt agtcggaagt attcaatcga
     5101 gaagttggga cggtaatgac ggtaaaaagc agtatgcgac ggaagttgtt gtagacgagg
     5161 cttactttac cggttcaaga gccgaatcag gtactcaagg cggaggaaat tatcagaacc
     5221 aaggctttaa tcagggcgga tttaacgctc ctcaatcgca gcctgcacaa ggcagtgaac
     5281 caaactttgg tgatgatttt gatatgggcg atttttcgga ccttgacggt tcggaagacg
     5341 atttgccatt ctaacatccg cgtgataaaa attgatagga ggaaaataac atggctgaaa
     5401 gaaatgacag accacagaga gcaagaagac ctaagaaaaa ggtttgtatg ttctgcgtag
     5461 ataaggttga gcatatcgat tataaggata cagcaaagct tagaagatac gttactgaaa
     5521 gaggcaagat cgtgccaaga cgtatcagcg gtaactgtgc aaagcaccaa agacagctta
     5581 caacagctat caagcgtgca agaattatag cactattgcc attcgttgct gaataattga
     5641 ttttttaaac ggaatgcact gcattccgtt tttttatata taatttaacg gaaggtgtta
     5701 taatgaacat aaaagtaaga gaatttaatg aaagcgatat acaagccgca aatgaaattt
     5761 ggaacgaagt cgtagaggac ggagtggctt ttccacagga agaatgtctt acggaaaaga
     5821 gcggattgga attttttaag agtcagtcat atacaggtat tgcgtatgat gaagaaacgg
     5881 gtgatattgt cggactttac atacttcatc cgaacaatgt cggcagatgc ggtcatatct
     5941 gcaatgcaag ctatgcggtg aaatcaacac aaagaggtaa gcatataggt gaaattcttg
     6001 tgacggattg cttgaaaaaa gcaaaggaaa tcggttacgg tgtacttcaa tttaatgctg
     6061 ttgtcgcaac taatcaatat gcacttaaat tatataaaaa actcggcttt gtacaactcg
     6121 gcgtaatacc taaaggtttc cgcatgaaag acggaacata cgaagatata attccgcatt
     6181 atcacacttt ataaaaattt ttttgaaaaa agtgcaacct tttggtgtgc tgaattgtat
     6241 tataagtgaa gggaggggga ttaatgacag acgaaaggtc aaaaacagac gtttcatctg
     6301 acgaagttat acgcaagtat tttgatacgg tgtataaact tgctttgtca caaacgaaaa
     6361 atcaaacttt tgccgaagat gtgacgcaag acgtgtttgt tcgatttata cagaataaag
     6421 ataaatttga gtcggaggaa catataaagg cttggctcat tcgggtgacg ataaattgct
     6481 caaagagtgt ttttatgtca tcgtggttca agaaaatcgt tccgcttgaa gaagatttaa
     6541 cctttgacaa tccagaaaaa agtgacgtgt atttcgctgt acaagacctt cccttaaaat
     6601 atcgtacggt gatacatctt ttctattatg aggatatgag cgtgagggaa atcgccgaaa
     6661 gcattgacat aaaagaatcg actgttaaat cgcatttaca cagaggcaga atgttgctta
     6721 aaaataaact gaaaggagat tatgactttg tttaggaaaa tttataaaca ggctaatgac
     6781 gatataaaac cgagtgaaga tatgataaac agagttatct tgaatgcgca taaaggtgca
     6841 gagaaaagac aatctcccct accgtatagg cgatacgcaa aatatgcggt atcggctgcg
     6901 gcggcagttg ttgttgtgtc tgcggcggtt atatctatgc cgtattggca gaaaacaaga
     6961 gatgacggcg tgattattga ggagcaagtt tcaacaccga caccgtccga tatgtcggaa
     7021 agtgtagttt caaatactgc ggatacttca tatccgtatc ctaccgatag tccccaaact
     7081 attcaaaatc cctatactcc ccctcccaat aactattcgg atagtcaaac taaaacggag
     7141 aaaaagaact gtgactataa gagtagtgac aatgaaaaag ttgcggtaaa taatcggata
     7201 gcaaacgaga acaaatcgct taaaatcgag gataaaacca aaatccccaa tatcgagagt
     7261 gaacaaaaca gtgaaaatca agaaataacg gaagatgtca ctgtcgaaga aagtgcaatc
     7321 gaggaaaaca caatcgaatt gtcaacaata ttttctgctt ttatgtcaag tgacagaaca
     7381 gcagaaatca gtacaaaaaa agaagtgccg accgaaatta caagcgatga aggcaatgcc
     7441 gatacggtta tcggcaccgg aatggcaatg agttcggcta tggctacatc agtgccgaca
     7501 cctcaaatag aaataaacgc tccgagcggt tttgaaacga ccgatacata taacggcggt
     7561 gcggcttttg aatcagaaaa cggcggaaga atagacgtaa attattatta cagcggaaat
     7621 gacgatacag agccggtgta taacgaatat gaaaatgatg tatcggcata ttttaccgaa
     7681 gataaaatcg attataatat ttacggaacc gatacgggca gagagcctgt ggaagaagta
     7741 gtcaattcaa tcaaataaag tattgaattg atttgcaatt tgtgatataa tataaacaat
     7801 ataatgattt tggaggtaat gattgtgaat aagaagataa tagcatctgt attggctgtc
     7861 gcatctgttt tgactgtatc ggcaggcgct ttggctgatg ataacattac ggttacggta
     7921 aacggaaata annnnnnnnn ntttcttttg tggaaataac accttttatt gaaaatgacc
     7981 atacacttgt tcctatgcgt gaaatattcg aggcacttgg cgcagacgta cagtgggaca
     8041 acgaaacaag aactgttata tcatatgatt cggtaagtga cgtaagtatt acaatgcaga
     8101 ttgattctga tgtaatgtat gtaaacggca acgcggttac acttgaaaca cccgcaaaaa
     8161 tcgtgggttc atcaacggtt gtgcctgtaa gagcaatcgc tgagggtatg cacagcgttg
     8221 tcggttggga cgaggcaaca aagacagtta ctgttgaaaa ggaaatcaaa acagagccgt
     8281 cttcacaaat tccaaaccca tggacagatt ataacagtct tgacgagctt aatgcggcat
     8341 tgaataatgc agagagcgta aaatatcaag tggcagattt gaacgcaagc agttttgatg
     8401 agggtagcaa gtgcgaggca caaagctaca gataccttgc cgacagtaat ttggcagaaa
     8461 tcaaatacac ttacaataca aatgtattag acattacagt tcgtacacaa ccgggtgacg
     8521 cagacatttc gggtatatcg ggcggtacta aggttgagga ctacaaagta gccgattcgg
     8581 cagttgaaat ttacacatac gataaaacaa catacgcaat atggtcatgc gaagacgcgg
     8641 gtacagtaat gagt
//
LOCUS       DAWACS010000016         8607 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_943_length_8607_cov_2.656922, whole genome shotgun sequence.
ACCESSION   DAWACS010000016 DAWACS010000000
VERSION     DAWACS010000016.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8607)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8607)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8607
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_943_length_8607_cov_2.656922"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1520..1529
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2305..2314
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2629..2638
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7932..7941
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2602 a         1804 c         1725 g         2436 t
ORIGIN      
        1 attcgtgcag gcgcacacga tacgcagttt atggtatacg actatgactg tgacggaaaa
       61 gccgaagttg cttgccgtac ggctgacggc acaatcgcag gtgacggctc tgttatcggt
      121 gactcaaaca aaaactatgc cgttgtaagc aacggtaaaa accttacagg cccgctttat
      181 cttacagtat tcaagggtga ggacggctct gtaattgata cagttgacta tgacccgcaa
      241 atcacaggca aaaccgcaag cggtcaaaaa tgggatataa gctcttgggg cgatacattc
      301 ggaaaccgtt ccgaaagata ccttgccgcc gttgcatatc ttgacggcac aagaccgagc
      361 atggtttttg caagaggtta ttacacaggt cctgagggtg aaacaggcgg cagaacagtt
      421 attgcaacat acgatcttgt agacggcaaa cttgttaaga aatggcgctt tgatacaatg
      481 gattacaaca ataaatatat cggacagggc aaccactcaa tgagtgccgc agacgttgat
      541 tatgacggtt gcgatgagct tatatacggt gcgcttgcaa tagacaacga cggcaagcct
      601 atgtattcga caggtttggg acacggtgac gcacagcacg tcagtgacct tgacacgtca
      661 agaccgggac ttgaagttta ctcttgtcac gaagaaactc aagcaaaata cagctttgaa
      721 atgcgtgacg cacgtacagg cgaaatactt gtcggcggcg aacaaatggg cagtgacaac
      781 ggtcgcggca catctgacga catagacccg cgttatccgg gttgcgaagg ctggtcagcc
      841 gcaggaatac ttaccgccgc agacggtacc gtcatttcaa caaaatacac tatgccggca
      901 aacttcctat gctattggga cggcgattta ggtcgtgaag ttcaggataa catttatatc
      961 agcaagtgga atcccgaaaa agaaaaggtt gaaactatct tcaccgcttc gggctgtact
     1021 tctgtaaacg gtacaaaagc taatccgtca cttaccgccg accttttcgg cgattggcgt
     1081 gaagaagtta tgtatccgct aaaggacggc agtgctttga gaatttacac aaccactaca
     1141 ccgacaagct acaaaattcc tacacttatg cacgacattc aatacagaat gcacgttgca
     1201 tatcaaaacg actgttacaa tcagcctaca cacctaagct attatcttgg ttttgacact
     1261 gaaaatgtac ctgtaccgca gatttataca gtaggcaaca atgaaaaaaa tcccgacctt
     1321 gcaaagaagt cttggaatat aaacgattta tacagcggtg aaaaggttga gcttgtactt
     1381 gacacaccga cagcacttac aaacggtgtt ccgaaacgtg ttgataacga cagcacggac
     1441 gttgtaccat accttgacga aaacgaaaga actcttgttc cgttgagatt tatttctgag
     1501 tcattcggcg cagacgttgn nnnnnnnnnc acaggaaatc aaaatcaacg gtgacgccgt
     1561 tacaatgaaa atcggcagca acgattatac tgtaaacaac gaaacaaaaa caatggacac
     1621 tgcaccgatt ttgaacaacg acagaacaat ggttccgctt cgtgcaatcg gtgaagcact
     1681 cggaaagatt gtttactaca atgataaata tatctgcata agcgatattg acttaaatat
     1741 gtcggacgat ttcgcaatgt caagaaaatc tacaatcaca tctgcaaaag tacctgataa
     1801 aattgaagtt gtcgcaataa acggaacagg tcaaaaatat taccctaacc aacttgacgt
     1861 ttttgccgtt gaggcaagcg acaatgacgg taacgttgaa acaggtgcgg ttgaccttga
     1921 tataaacacg cgttggtcaa gttacggtaa gagtacgctt acgcttgatt taggcgaaga
     1981 aaaggacgtt tcgggtgttg caatcgcaat gtggaaaggc aatgaaagaa tttatccgtt
     2041 cacaatcgaa tactcggttg acggcaagac ttgggaaact gcacttccaa aaactcaaaa
     2101 cacaggtgaa tcaagcgaat ttgaaaagta tatgttccct gaaacggtaa aagcacgtta
     2161 tatccgctac aacggtgacg gtgcaacaga ccctgacaag aattactgtc atatcagcga
     2221 aatcgctgtt cttggcgctg aagaataaaa aaaggaactg tcgacaggct gacagaggtc
     2281 gagaaattag ttcggatttt gtgannnnnn nnnncaaaaa taatattttg cacgttccgg
     2341 acaatttgtc gacagtctga aacggtgttg cattgcaaca ccgttttttt gtttgaattg
     2401 gacaaaacat ttctattatg atataattaa aataatatat actgtaaagg cagagatgat
     2461 atgaacagag ttgtaattgg aatacttgca cacgttgact cgggtaaaac tacgctatct
     2521 gagggaatgt tatacagtgc aggtgaaata cgaaaaatcg gtcgtgttga ccatggtgac
     2581 gcatttctcg attcacacga aattgaacgc gacaaaggca ttacgatann nnnnnnnntc
     2641 aaagcaagcc gttatgcgtt ttcgtgacac cgcatttact ttacttgaca cccccggtca
     2701 cgttgacttt tctactgaaa tggagcgtac tttgagcgtg cttgattacg caatcctcgt
     2761 tataagcggt acggacggga tacaaaatca tacagaaaca ctatggcggc ttttggcacg
     2821 atacaatgta cctacattta tattcgtgaa caaaatggac ttaaacgccg acagaaacgc
     2881 cgttcttgat gaattgcata caagattttc ggacggttgt attgatttta cgcaaaatca
     2941 tgaaaatgaa gatttcagag aatctctcgc tatgtgtgac gaagctataa tgaatacatt
     3001 tcttgcggac ggtactgtca aaaatcaaga cataagaaat gccgttgtac aaagaaaaat
     3061 attcccgtgc tatttcggct ctgcacttaa aatggaaggt gtatcggaat ttcttgaagg
     3121 acttgaactg tatacaagac aaattattta cgataacaaa ttcggtgcaa aggtattcaa
     3181 aattgccgaa gatgagcaag gcacacgtct tacttatatg aaaattaccg gcggtacttt
     3241 aaaggtaaaa acacttttaa agggtaacaa gaaaaatgag tggtcggaaa aagtaaatca
     3301 aatcagaatt tattccggtg caaaattcca agcggttgac gaggcattcc ccggtacggt
     3361 ttgtgccgta acaggtctta cacagacata ttccggtgaa ggtataggtt gtgagcctga
     3421 ttcaaaaaat gccgttcttg agcctgtttt gacgtaccgt atggagctta cggacggtat
     3481 tgacgtgcat acagcactta ccgaattacg tcatttggag gacgaagacc cacagcttca
     3541 tataatatgg aacgaacagt tgcaggaaat tcacgttcaa gttatgggcg aagttcagct
     3601 tgaagtttta aaacgaatta taaaagagag attcggtatt gatattgaat tcagtcacgg
     3661 cggtattgcg tatcgtgaaa caatagccgc acctgttgag ggtgtcggac attatgagcc
     3721 gttaaggcat tatgctgaag tgcatttact tatggagccg actgaaaaag gcagcggtat
     3781 gcagtttgcg gtaaattgca gtgaggacag tcttgaccgc aactggcaac gacttattct
     3841 cacccatcta aaagaaaaag cacatatcgg tgttctgaca ggctcaccga ttaccgatat
     3901 gaagataacg cttatcgcag gacgcgcaca ccaaaaacat actgagggtg gcgacttccg
     3961 tcaagctact tatcgtgcgg tacgtcaagg cttgaaaatg gctgaaagtg ttttgcttga
     4021 gccgtggtac gaatttcatc ttgaaatacc taccgaaaac gtaggacgtg caatgaccga
     4081 cattcagcaa atgggcggta cattcagtca acctgaaaca atcggtgata tgacgcgaat
     4141 ttcaggctct gcacccgttg cgacaatgcg tgactatcaa atggacgtaa caggttacac
     4201 gcacggcaaa ggcagattaa actgtatttt aagcggttat gagccttgcc acaacaccga
     4261 agaagtgatt gccgaaatcg cttacgacag cgaaaccgat attgaaaatc ccgccgattc
     4321 ggttttctgt tcgcacggtg caggctttgt tgtaaagtgg gataaggttt acgaccatat
     4381 gcacattgac ggcataaagc ttgaccaaga cgatgatgaa gaagaaaacg tctatcagcg
     4441 tgcaaacgac tacatcaata tggttgccga tgataatgag cttatgcaga tttttgaacg
     4501 tacatacggt cctgtacgcc gtaaagtagc gtcatacact gtaaagaaat ccgccaccga
     4561 acagaaaaaa catcaaaaat caaagcctgt taaattaggt gacgaatatt tgcttgtaga
     4621 cggttacaat ataatctttg cgtgggacga actgaaagaa ctcgccaaat ataaccttaa
     4681 tatggcacgt gaccgactga ttgatattct ttgcaattat caaggcttta agcaatgcaa
     4741 tttaatactt gtttttgatg cgtataaagt taaaggaaat gtcggcagtg ccgaaaaaat
     4801 tcacaatata aacgttgtgt acacaaaaga ggctgaaacc gccgatatgt atattgaaaa
     4861 aatcacacat gaaatcggta aaaaacatcg tgtgcgtgtt gccacttcgg ataaccttga
     4921 acaaattatc attctaggca acggtgcgac acgactttcc gcaaatgaac tgttacagga
     4981 agtaaaactc gctgaaaaaa ctattatgga tattataaat tcaaactaaa aaaggaagtt
     5041 ttgaaaactt ccttttttaa tatttcgcat tttagtaatt gtatgatccg agaaatttat
     5101 agaaaaatgt attctgtctt actttatcaa gtgcaaagta aattgacgca tcatcaatat
     5161 tgccgtcaat atcaatgaag aacacatact taccgagttc ctttttcata ggacgtgact
     5221 caattttagt catattaatt ccggacttcg ccaaaagctc aatcgcactg tacaagctgc
     5281 ccggtttatt atcaatcgca aatacgattg atgacttatc gtgacctgta actgtcttat
     5341 tcggtgtttt ttcaataata acaaatcgag ttgccgatac acgccacaga gccgtccgat
     5401 tcttttacac gttttgccgc agacgcagtt gaatcggcaa attcaatttt cacgtccgca
     5461 aattcgtgcg acaataattt tgaacactgt cctatcggtt gagggtggga agtgataatt
     5521 tttatatcct ctttctttac acctttttta accataagat tttcgcttat atgaagaata
     5581 tattcacctg ttatatataa atccacatca aaagcaagag tatccaaagt tgtgttgata
     5641 cttccgtcaa tagaattttc aatcggcact attgccctgt ctatctcgcc tttttcaacc
     5701 gccaaaatta cagaataaat cgttgaaaac tcctttattt cttctttacc ctcagaccat
     5761 tccatagccg cctgatgtga aaatgtcccg ttcggaccaa gataacctaa catattaagt
     5821 caataactcc cttcaataaa acgaaaggct gaaataaaat tgttcacaca atgtgggcaa
     5881 cttcatccag cctataaatt tcttataata gaatcttatt tactgccttc gcgattggat
     5941 caagctcttt cataagcttg tcaaactttt caggtgtaag cgattgtgcg ccgtctgacg
     6001 cagccttttc agggcagtta tgtacttcaa tcataagacc gtcagcacct gccgcaatag
     6061 caccctttgc aatagccgga acatacttat atgtacctgt tgcatggctc ggatcggcaa
     6121 taataggaag atgagaaagt tcctttgaaa caggaattgc cgaaatgtca aatgtatttc
     6181 ttgtagcagt ttcgtatgtt ctgatacctc tttcacaaag gattaccttt tcattaccct
     6241 cagacataat atattcggca gccattagcc actcttccat agtacaagca agacctctct
     6301 ttagaagtat aggcttgtcg cacttaccga cttcacgaag aagtttgaag ttctgcatat
     6361 ttcttgcacc gatttgcata atatcagcat actttgcaac aagttcaaca tctcttgtat
     6421 caagtacttc agtacagata ggcatattaa gttcctgacc gctgttgtac ataatcttca
     6481 agccgtcacc ctcaagacct tggaatgaat atggtgatga acgtggcttg aacgcaccgc
     6541 cgcgaagaat gtttgcaccc gaggccttaa cagcctttga tacagttgtg aactgttcct
     6601 ggctttcgat tgcgcaagga cctgcaaata ctgcaagctg cttgccaccg actgttattc
     6661 cgtgaccgat ttcgataact gacggttctt tgtgaagctc cttagatgca agtttatatg
     6721 gtttcataat cggaacaacg ttttcaacac cgtccatagt aagaatttga ttcatactca
     6781 aaagacgctt gtcgccgatt gcgccgataa ctgttttctt ttcaccttga atagggtgcg
     6841 tttggaatcc gaactcggca agtagttttt caacactctc gatttccttt gccgatgcgg
     6901 attctttcat aataataatc atttatgttc acattctttc tttaataatt acaacatata
     6961 cagtatagca aaaaaggaca atttttgcaa gttagtttta aataatttca gaaacatctg
     7021 caaaaacagc taccgttctg tatgttccgt tgtaacattt gcactgttta ttaaagtaca
     7081 gccgctttcc gtcacgcgaa aacgacggat ttggatgtac ctgaagtttc catgtggtgt
     7141 attcgtcctg cgatatttca aaagtcgcaa tcggattttt gtcaccgcgc ttgtacaaat
     7201 aaacattcgg tgcaaagtcg ggataacatc tgtcaccggc aaaatatttt ctgtcaaaac
     7261 ttccgtccat atgatttgaa attccgccaa gcacttctaa ttcctctccg tcctttgaaa
     7321 aacgtgcaat gtagcttgtt tccatttcaa tgtgtctgcc ttgattaaac tgacgcgttg
     7381 ccatatatgt gtcgtcgtca taccaaagct gatgtacagg cttgtccgct atcatatgcg
     7441 tttttttagt gtctaaatct atacaaccca acgcaccgaa tacagggcag tcctttacgc
     7501 ccaagcgcat cataactgcc gttgcggacg gatttagctg aacgtggctg acagacgcgg
     7561 tatgctcgtt cggagtaaga ccgtgtttcc ttaccatatt taaaatatct tcctctgttg
     7621 caacaagcgt tatttttccg ctttcaacat caagtatgta aattccgcac gaattaattt
     7681 cgggataatc gggatttttg tcaagatatt ctgtcgatat tgaaaacgga tacttgccgt
     7741 tttccgcaca atgactttct ttgccgcgaa ttttgtactt aacctcaccc gtatcaacat
     7801 tgagtatacg gaatacacat atagacggct ctttgccggc tatgccctca aattccacat
     7861 cacgaaaaac tataaggctg tttgttataa aggttgctga cgggccgttg tgattgccgc
     7921 agtgtacatc annnnnnnnn ngtacgtcat ataccttgcg gtggttttca aaatttctgt
     7981 cgcatatcca tatttccgtt tcaggctttg tcaagtctgc tttgcgtgca tatataattc
     8041 tttttgaatc cgggctctca accattccgc cgttcattcc aagcaatgtc gaacccgaat
     8101 aatcacatat ttcctttact ttcatctctc tcacctcatt atatgaatta taccataata
     8161 ttgagatgtt ttcaatattg gttatttttt ctttttcggt gtcggtaagt cgtcatatat
     8221 cgccttaaat aattcggtta aatattcttt gttgtcaact tcttctatta aaatcatttc
     8281 tttcgcaccc tcataaggta cttcgtatcg aatgtcagaa acatagtcaa tagccgattt
     8341 aacaggtttt ataagcagtc ggtcgtcata tattcccccc gcaattctgc ctttgtagta
     8401 aataatgtac tcccccatca tcgccctgta cgttatttcc tctacctccg ataattgctc
     8461 taatatgtat tctaaaaatt ctttactcga tgccatctgt ttctcctttt tcttatatat
     8521 cactttcata tacaaatttt aaaatataat tgttatcaac gcattccaca accacattgt
     8581 atttttcatt ttctgataat tcatatc
//
LOCUS       DAWACS010000017         8547 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_949_length_8547_cov_2.873881, whole genome shotgun sequence.
ACCESSION   DAWACS010000017 DAWACS010000000
VERSION     DAWACS010000017.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8547)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8547)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8547
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_949_length_8547_cov_2.873881"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8149..8158
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2302 a         2010 c         1451 g         2774 t
ORIGIN      
        1 tacatttttc gcataaaaaa agctactgta tgaaagtagc ttttttgtct gttatccctg
       61 tttttctttt gttcttttaa tgattttcaa acgagtatgc tcctcacgtt ctttttcttc
      121 aagagcattc tgaatatcct tagacagttt atcataaaga ggtatcgtaa tattttcaag
      181 agcatttgct ctcttttgcg ttttctttat gttcatagcc aaacggtaaa ccgcattttc
      241 aatttccgta agcttaaccg tcatcacttt aacttcgtta aatttaacgc ttgcctcgtc
      301 aagtgccgag gacgttccgt taaatccgta aacaggtctg ccgtcaggct tatcagccgt
      361 aacaacaggt atttcaacgc ccataacact gcgtaaatca acttttacgc tgttttcaag
      421 cactgccgaa tgagcaaggt tctgcacctt gctcacaccc attattacgt ttgccgattc
      481 caaagcctta tatgcagcgg caaaggtttc ttcgattttc gcctcgactt cctttgcctc
      541 ctctatcaaa agcatcattt ctctgattag gatatttcgt tttttatcaa gcatttcata
      601 gccttgcttt gaaagacgca atgtgttttt tgccatcatc aagttacctt ttgtaggtgc
      661 taacgtcttt gccatctgaa attccccctc tcaggattat acttcttcgt cctccacagg
      721 acgataatat ttttcaagag tcttatcgct gactctgtca agttccctct taggcagcat
      781 acccaaaagc tcccaaccaa gatttaaagt atcgtcaata gttctgtttt cattcttacc
      841 ttgtgtaagg aacttttcct caaacgctct gccgaacttg atatacttct tatccatatc
      901 ggaaagttca tcttcaccga taactgacgc caaactcttt gcgtcctcaa cctttgaatc
      961 agggtggtcg gctcttgtaa agccgtcacc gataccgtcc ttcatcaaac gcgaaagtga
     1021 cggaagaatt gaaacaggcg gatatacgcc tttaaggcta agctctctgc ccaaaactat
     1081 ctgtccctct gtaatgtaac cggtaaggtc agggattggg tgagtaatat catcgttagg
     1141 cattgtcaaa atcggcactt gtgtaaccga tccctttgcg tccttgataa tacctgcacg
     1201 ttcataaagt gaagccaaat ctgaatacag ataaccaggg aaaccctttc ttgacggaat
     1261 ttcacccttt gaagatgaaa cctcacgaag tgcctccgcg tatgacgtca tatcggtaag
     1321 tattacaagt acgtgcatat cgtgcttaaa tgcaagatat tctgccgctg taagcgcaca
     1381 tctcggtgta ataattcttt caacgacagg gtcatttgaa agatttaaga acattacaac
     1441 cttacccaaa acaccgcttt cttcaaatga ctttttaaag tagtttgcaa cgtcatgctt
     1501 aacgcccatt gccgcgaata cgatagcgaa cttttctccg ttttcgtcat cagggtcaac
     1561 aacctttgcc tgtcttgcaa tctgtacggc aaggtcgtca tgcggaagac cgtcgcctga
     1621 gaatatagga agcttttgac ctcttataag tgtggcaaga cagtctattg acgaaatacc
     1681 tgtctgaata aagttgttcg ggtattcacg cgaaacggga ttgataggct gaccgtttac
     1741 gtcacgaact tcgtccgcaa agtaatcgcc caaaccgtca atcggtctgc cgataccgtc
     1801 aagcgttctg ccaagcattt cttttgaaag cggcaaacgc atcggctcgc ccataaattt
     1861 tgttttagtg ttggtaagtg acagatttga agtaccctca aaaacctgta ttaccgcttt
     1921 atcaccggaa atctcaataa ctcgtcctat tcttttctcg ccgttgtcaa gagtaactgt
     1981 aaccatttct tcatagaatg tatcgtctac tccctcgata actgccaaag gaccctgaat
     2041 attattaaga cccatatatt ctattgccat atgctcatat cctttccttt agctttcatg
     2101 gttataaatc aacttgtcaa atgtttcgtt gatttttctc tgtaaatcat ggaacggacc
     2161 atcaatattg tcatttccaa tcgtgtattt aattctgata atgtcctcgg caattccgct
     2221 ttcgctcacc tttgaaagca caacgccctt ttcgacacat tctttaattc tcttatcaaa
     2281 aagaagaatt gtatccatca tcttattttg cttttcaagc ggtacatatg tatcgtccgc
     2341 atggaaagca ttttgttgca agaaacctac tctgattgtc tttgcaacct ccaaagtagc
     2401 cttttgactg tcaggaagaa cgtctgcacc gattagctta acgatttcca taagactgct
     2461 ttcttcttgc aaaattgcca tcatcttatt tctcttttcc ataaagtccg gagaaacatt
     2521 ttgagcatac caatcagtaa cttcaccgat atattcactg taactgttaa gccactgaat
     2581 agccggatag tgtcttgcat atgcaagcga cttgtcaagt gcccagaaac atctgataaa
     2641 acgctttgta ttctgtgtta ccggttctga gaagtcaccg ccctgtggtg ataccgcacc
     2701 gataattgat acagaaccct cactgccgtt aagatttgta acatatcccg ctctttcata
     2761 gaaagccgaa agtcttgacg caagatatgc agggaaacct tcttccgcag gcatttcttc
     2821 aagacgaccc gatatttcac gaagtgcctc tgcccaacga gatgttgagt cagccataag
     2881 tgcaacgtca taacccatat ctctgtaata ttccgcgatt gtaacacctg tgtaaatact
     2941 tgcttcacgc gccgcaaccg gcatatttga agtattagca ataagcattg ttctgtccaa
     3001 aagcggtctg tttgacttag ggtcgataag ctctgagaac tcgtcaagta cctgtgtcat
     3061 ttcgttacca cgttcaccgc agccgatata tacgataata tccgcatcac accactttgc
     3121 aagctggtgc tgtgtcatag ttttaccggt accgaaaccg cccggaacag ccgccgcacc
     3181 gccttttgca agcgggaaca ttgtatcgat tactctctga cccgttacaa gcggtcttga
     3241 aataggctga cgctgtgtat aaggtcttgc ctttttgata ggccattttt gtttcattgt
     3301 tatgtcgata atttcgtcat cgctctttac ctttgcgata atatcaacaa tagtgtagtt
     3361 gccgttcgga gcaatccatt cgattttacc tttgatattc ggcggaatca taattctgtg
     3421 ctcgataagg cttgtttcct gtgttacggc aatgatgtca ccgcctgtaa catagtcgcc
     3481 ttgtttcgca accatttcca cattccaagt ttttctttcg tcaaggttag taacctttaa
     3541 acctctgccg atgaatgagc ctgacatatt tcttatttct ttcaacggtc tttcaatacc
     3601 gtcaaaaata tcagacaata tacccggagc aagagttatt gacaaaggac cgcccgtacc
     3661 gactacaggt tcaccctttt taagacctgt cgtttcctcg taaacctgta caatagtagt
     3721 atcggaagtt acgccgataa cttcgccgat aagttcttca ttaccgacat ataccatttc
     3781 ctgcatttca aaggaatctg aaccctttac ttttacgacc gggccgttta taccgaatat
     3841 ctgacctttt gtattagcca tttaccttca ttcctttccg tttgtatgcc gtttaaccca
     3901 gtgccaatcc actgctttga aggaatttct gcttttcttc tgacagcatt tccttgaatg
     3961 aatagtcaac agcaaccttt ctgtctgtat ttaaaacttt tgcaccgcca agaaaatctt
     4021 tttcacccga tgcctcaacc gtaattcttg acgtgtcggg aatctgttca attttttctt
     4081 taagctttat atcgtctgat gagatataaa ttgtctttga accttttccg acttcaaata
     4141 aagccttttc aatcttgctt aaaagccatt cttcgtattt atcgctctcg ataaattcaa
     4201 cgatttttgc tctcgaaagt ttcattacgt catcaataat ttcttcacga cgcatgataa
     4261 gttttttctt tgaatcaagc tctgcgtgaa gaactctctc atttgcactt ttttgagcgg
     4321 attgaatatt ctgctgaata ttctcatatg caccctgcaa aagctcgttt tcctttgcat
     4381 ctattctctc tgaatattcc ttttgaacat tctcaagtaa tttttctctt ttctgttccg
     4441 catctttgag aacaagcgat gtaaaggtat tgagtttttc tgttaagtta ttgctttcca
     4501 taatccttac ctcccctgtt taaagtttaa gaccgattga ctcttgaacc atattgttga
     4561 tactgttagc aatatcacga ctgccgtgtc tgtccggtat ttctatgata agcggagtgt
     4621 gcaatgtaat tttaagatgg ttaagatatt ctttaaccat tgccgccgct ttttccgtaa
     4681 atatcaaaat accaagttca gggtcgttta cagccttttt aatttctgca gtaatagttt
     4741 cgctgtcgtg agcaacacat ccgtctatac ccacaagtcg cataccgatt tgtgtatcaa
     4801 tgttgtcact gattaaatac attttcataa aacatacgtc ctttcagact tgtaataata
     4861 aaatccattc tccgtccact ccgtaacgga ctacactcgt cgtccatcgc ctccccttga
     4921 ggggaggtgt catcgcagat gacggttggg tggctcgctc gcccgattta ccgaaggctg
     4981 aaagaaaatt gttcacagtg ccgctctttg cagcttgcgg tgctgtgggc aattttactc
     5041 agcctattat gaattaagaa tcaatataga gattacaaga ccgtatagtg cgataccttc
     5101 tgcaagggca acgaagataa gagttttacc cataatcttt tcattttcac tcattgcacc
     5161 gatagcagcc gatgcagctg atgcaacggc gataccaccg ccgacaccgc taagacctgt
     5221 tgaaagagcc gcaccgatga actttagacc ttcaccgact gtaagaccgg cttcagcacc
     5281 tgtacctgcc gcaagagcgc taactccgcc gccaagtgtg cagataacgc cgacaccaag
     5341 aatactgaag aatgagattg cgttcatcaa aaggcattcc ttaccttttg tcttgcctga
     5401 cttataatac aaagcaacag gaacgattac tgtaagtgcg attaaagcga ataaaatttt
     5461 cattaacata gtgattacct ccaaaatata ttaaaaccaa tttatattat ttatcacgaa
     5521 gtgacataaa ttctttacct ctgcctgaga agtatcttga gaacatttcg taatactcca
     5581 gacgaagtac ctgtattccg acgataagac cttcaagaac cataacaagt acgttaccga
     5641 ttacttgtac aataactccg cctacaccgc cgctttgaga aagaattgca acaaccatca
     5701 tcatacctac atggactatt gcaaaagcgc ctattcttag gaatgagatt gtgtttgtga
     5761 agaaactcaa aatcacttca aacatttcaa acaagttttc cacaaagaac attccgtttc
     5821 tcggcagcca gtccttttta ccctctacaa gcttgccaag cggctcgcat aggtacatac
     5881 aaagcactgc caaaataata attgccacaa atacgaatgt cggtataccc cactttaaga
     5941 acaagttacc ggcaagaaga agtgcgccta tatagaacac caagcccgcg acaccgttat
     6001 gtccgaacag tgcttcgcca agttccttac ttttcagtgc gtttataaca ttcaatacca
     6061 taccgaatat aattatcagc acacccatac caatcgtacc gcccagcatt aacgctattt
     6121 cattcatagg gtttaacgct gttgtatgga acaattcagg gattatttct tcatttccga
     6181 agaaacttcc gtatataaat ccgaatatca caccggatat tccgaccatt ccgacaatac
     6241 cgcccaagtc ccatttcttg actttgtaaa ctataaagcc tgctatcgca agcacaaggc
     6301 tctgacctac gtcaccgaac attataccga agaacagtat atacgttacg gcaagtatgc
     6361 ctgtcggatc aatttccgta taactcggaa gtccatacat tttaacgaac atttcaaacg
     6421 gtttaaatac aggattattt ttcagctttg taggcggctg aatatccttg acgtcctctg
     6481 catcctccat atagaacatc acgacgtcat ctgaaccgtt aatctctttt tcaagcgact
     6541 ttgcactctt ttcatccatc caacctacta tatagaagaa catatcgccg tgaatggcat
     6601 ttcttcttac ttcggagaat tgatgacgtt tcttcgcaag attgtaaatt ccgcaaagtt
     6661 tatccgcact gcttgaaatc atctcggcgg tctttgcact gagttcttca atctgctgtt
     6721 ttaatttttg attttggttt aataatccac gctttatttc aagcggagta ccaacgtaat
     6781 cctctgatat gtccataggt tcaaaataca gagaattaaa tacttcttca atctttctct
     6841 ctttcaaaag cggtgcgaag taaaagcccc atacatctgt tgcgtcctcc gcagtcttaa
     6901 tgaagaaagt ttcaagattg tcaaggtagg ttataagcgt tttataacct gttttcggaa
     6961 tatgtccgaa tctgcatctt ataaattcaa acttgaaaat tttcgacaaa tccacgtcca
     7021 tactgagcat tagattaagc tgctcaaccg ccgcttcatt tgcctttatt ctctctgaaa
     7081 gctcgtcgct ttggttacgc tcttcttcga tacgctcgtt tattccgtca ataaaatttt
     7141 gcatatcgcc gagctttaca ctttcgggag caatcaattt cttgttcact gtgtagtttg
     7201 caagattcat aatgcttaac gcattttttg caactatgtc gtattcattg ttttcctcaa
     7261 agtttttcag cttgcctctg tttgaaatta ccgacatagc attttcaaga tgaatatctc
     7321 tgccgtaaac gtacttttca acgaccgtat caaattcact gatttgagcg gcaatagtta
     7381 cggctttcat ctttacgatt gccattttcc ccctcctcta ctcaagacca atatgtgtgc
     7441 ggataaaatc aggatttaag ttgtatctta tcccctcgat aatggtcgta atgttattaa
     7501 gttcaacttc ctttaggttc atataggcat ataccgcaac catcgactct ggatgagtta
     7561 caaatatact ttttgataac cggttataaa tatacctgta attaccttcg ggaaatcttc
     7621 ccttatcata atcttcaaac aactttccgt atgcagtcga ttgatttaca atatcaataa
     7681 acctttcaac agaatctgca tttacaagct gtttgattac ctcttccgtc aatctgtatc
     7741 tgacaggcag aagataggtg aaaattattt catttgtaaa ttcaaaatat tttttgcccc
     7801 tgtaaatcca cataagattc agcatatcta tttttgttcc gataagcctt ttaaataaat
     7861 tctcctgtgt cttatcaaga gcgaccgatg ctgtatgcca tattgactta tagtaatatg
     7921 tgtccagcac catacccatc gaaaatgaat ccgcgcctat attttccgct cgttgcagcg
     7981 gctcgctgta aggagtgttt ttacaagcct cgatacaatc cgacagggat tttgcattat
     8041 gcattatctc acgatttatt ttcgtgtgtg tttcaaagaa agcgtcaaat ttgctttggt
     8101 ttacctcatc attacttctt tcctcgtgag tatagatata tcttatctnn nnnnnnnnaa
     8161 attatatagt ctgacgtatt cgtcaaccat ttcctgttca agcaggattt ccatctgacc
     8221 tctgtgaata tctctctcat tcacctgttc aagaacttca ccgtagccgg gtgtactttt
     8281 aagataactg catatgtcat ttacgctttt tctttcaagg agctgttcat agtcatctct
     8341 tgtcagaaat tttgaataca tcgcctttaa tttagcggcg attgcactgt attccacgct
     8401 cattttgctg ccacctcact tatctgccga taatattctg tacaatttca ttaacccatt
     8461 tgtctttatt gtcgttgtac ttcttttcga gttcggaaag atgtctttcc atatttactc
     8521 ttatggcctc tactttttta tcggcat
//
LOCUS       DAWACS010000018         8312 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_983_length_8312_cov_2.701344, whole genome shotgun sequence.
ACCESSION   DAWACS010000018 DAWACS010000000
VERSION     DAWACS010000018.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8312)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8312)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8312
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_983_length_8312_cov_2.701344"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1387..1396
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2131..2140
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3224..3323
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5717..5726
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2634 a         1366 c         1763 g         2419 t
ORIGIN      
        1 cttgttcttg ttctcggaag ttaaatttga tacattgaaa cttgaccgtg aacttgttca
       61 taagcttgaa acagatgaaa agacttgtat gatttcaaag tcggttatag atatttgtaa
      121 aaattataat atttccgttg tcgccgaggg cgtcgaaaat gaaaaggaat ataatatttt
      181 gaaagaaatg ggctgcgatg aggcacaggg atatttgttc gataaaccga tgtcgtggaa
      241 tagatttgaa gaaaaatatt tgtaatttaa aaggcggtac atatgtaccg cctttttacg
      301 tggttggaaa atgttgactt tataattttt tcgtactata atattattta gaaaatttta
      361 tgttttaata aaacaaaaag gggaacaagc aaatgaaaaa gaaaatttta agttttgtac
      421 tcgtattgtc aatgctgtgc gcatttatgc cggttattgc cgtagcagaa acaagcggta
      481 cttgcggtga caatgtgact tggacgcttg accaaaatgg tacgctaaca attagcggtg
      541 caggtgatat ggagaattct gcattttata ataataacaa tataaaatca gttataatag
      601 aaaatggtgt aacgagttta ggtgattctg actttatggg ttgtcaggag ttgacaagta
      661 taataatgcc tgatagcata ataagaatac cagataatat attttatgga tgcgttaaac
      721 tggtgaatgt gataattcct aatagtgtat cagagattgg tgattgcgca ttttactatt
      781 gtagtagttt aacaaatata attattccta acaatgtaac aagtattggc tattgcgcat
      841 tttgctattg cggcaaacta acagatataa caataggaag taaagtgaaa caggtgggaa
      901 attctgcatt tggtaattgc aagaagttat caagcgtata tataacagac ctttcggcat
      961 atttaaatat aaattttgat aataattatt cgaatccgat atattatgct aataaattgt
     1021 atttgaataa gaaaagagtt gttggcgagg taacaatacc agttggtgta aaacaaatac
     1081 ctatatatgc ttttgccggt ttggacagta taacaagcat tataatacca gattgcgtaa
     1141 caagtatagg ctatgctgca tttaatggct gtgatagact agcaaacgta acaataggga
     1201 gcggaataac aagaatagaa gataatgttt ttaaagactg taataactta gaaattataa
     1261 attataatgc cagtaaagtg tttcctgatt tatttagtgg ctgtactgta tcgtcagtat
     1321 ttttgggaaa ctatgtagca agtatagaag aaaatgcggt taataattgt agcaatttaa
     1381 acaagannnn nnnnnngata tatataccga aaaatgtaac agaaatagaa aaaggggcat
     1441 ttgctggttg tgcaaattta actactgttg aatatagcgg taatcaatct gattggaacg
     1501 aaatatatat tggtacggat aatgaaaatt taacaaacgc aactataaat tataataatg
     1561 cactgacccc gaaaccaacc ccgataccta ttacaaaagc aacgattaca aaaacagaaa
     1621 cagatattac atatgatttt gatgttgcga cggaaaatac atatgaagat tgttatgttt
     1681 atgtggcggt gtatgatgaa aacggaatat tgtgtgcgat aaatcgtgta ccacttgaaa
     1741 caacgggtaa tacaagtgtt tcggttgata aaaatgataa tgcaaaaaaa gcaaaagtct
     1801 ttgttttttc gagtacattg caaccgatta tcacatctga ggaatttcct ttgatatgac
     1861 gaaaaaagag cttgcatatg caagctcttt taaaattctc tgtttttgat taaccaaaat
     1921 atctctttaa cataccttca aagcctttgc cgtgtcttgc ctcgtctttt gccatttcgt
     1981 gaactgtgtc gtgaatagcg tcatagccaa gttcttttgc tctctttgca agttcaagtt
     2041 tacctgctgt tgcaccacat tcagcttctg ctctaagttc aagatttttc tttgttgacg
     2101 gagttacaac ttcgccaaga agttcggcaa nnnnnnnnnn ttcggcaaat tttgcagcgt
     2161 gttcagcctc ttccaaagcg gctctcatat aaaattcgcc gatttcagga taaccctcac
     2221 ggattgcctg tctgctcatt gcgatataca taccaacttc tgtacattca ccttcaaaat
     2281 tagcctttag accttcgtaa acatcagctt caacctgatc ttttgcacct actccgataa
     2341 cgtgttcaca tacaaagttt agtgcagtac cctcttccat aacagtgaac tttgaaccag
     2401 gaacaccgca ctgtggacat ttttcaggtg gttcgttacc ctcgtgaaca taaccgcata
     2461 ccggacatac atactttttc ataataaatt tcctccttag aattaattat tttttttact
     2521 actacaattt tcgcagatac cgtaaaatat aagcgaatag tcttctatat ctgcatttaa
     2581 tttttccgcc gcttgatttt ttatctcgtc cataccgtct aaatcaatat cgtatatttt
     2641 gtcacactta ttacatacga aatgaaaatg tcggtggaca aaaccgtcga aatgttcttt
     2701 gtttgcggaa gtgctgattt ttaaaatatc tccgttttcc gcaagctgtg caaggtttct
     2761 gtatacagtt ccaagactga tgtttggaaa ttccttacgc agattttcgt acagccaatc
     2821 agccgacggg tgggagcgtg tacttctaag cagcgttaga agtgcctcac gctgttttga
     2881 atacttcata catttcactc cttcaaaatc aataatgata atcattactg atttatgagt
     2941 tcattataac agacgttttt cggtttgtca atagtttttt gaaataaatt tagattaatt
     3001 ttgtgtaaat ttgattttgg gtattgccac cactcctcag ggagaaaaaa aaggctccca
     3061 tagaggggag ctgtcggcac agctgactga ggggtggttg cgtgtggcaa acgatgattg
     3121 cccctacgga gtgtggttga tgaatagcca ccactccgtc actgcgttcc acctctcctc
     3181 aaggagaggc aagaaaggct cccatagagg ggagctgtcg gcannnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnccacctc tcctcaagga gaggcaagaa aggctcccat
     3361 agaggggagc tgtcggcaca gccgactgag gggtggtttc gcacattgca aaatactttt
     3421 ttattctaaa atttacgata ttttttgatt agtcttgatt ttatcggcgg tttataatat
     3481 aatatagaag taaaattatg tttacaggaa aggaactgat aaatatgaag aaaatggaac
     3541 agctttacga aggaaaagca aagaaggttt ttaaaactga tgacgaaaat ctttacatcg
     3601 ttgattacaa agatgacgca actgcattta acggtcttaa aaaaggtcaa attgcaggta
     3661 agggtgttgt gaacaacaaa atgtcaaact tccttatgca aattatggag aagaacggta
     3721 tccctactca ctttgttgaa gaaatttcag acagagaaac tgttgttaag agagtagaaa
     3781 tcgtaccttt ggaagtaatc atcagaaata ttgcggcagg tagtttttca aagcgttttg
     3841 gcgttgaaga aggcaagaaa ttaaactgca caactttgga atactgtcta aagaacgacg
     3901 accttggcga tcctatgatt aacgattatc agattatcgc tatcggtgcc gctactaaag
     3961 aggatcttga tactattgct gaccttactt ttaaagtaaa tgatgttcta aaagcatatt
     4021 ttgaaagtat taatatagaa cttgttgatt tcaagattga gttcggtaaa actcctgacg
     4081 gtcagattat tcttgctgat gaaatctcac ctgatacttg ccgtctatgg gacgctacaa
     4141 ctcacgaaaa acttgataaa gaccgtttca gacgtgatat gggcaatgtt gaagaggcat
     4201 atgcagaggt atttaaacgt cttggactat aattaagtgt agaccgaatt aaattgcccg
     4261 gctcaccgct ttttgccgag ggcagtgcat atgtacggca ccgtcgactg caaagtggca
     4321 atcaaagcga ttttcttcgg tcttataaaa aatttagagg agtaaatgaa atggcaacaa
     4381 atacttacga aagtccgtta aactcacgtt acgcgtcaaa ggaaatgcag tacatttttt
     4441 ctccggacaa gaaattctcg acttggagaa aactttggat tgcgcttgca gaaagtgaaa
     4501 aagaacttgg acttgatata acagacaaac agattgctca aatgaaagag catatttacg
     4561 atattaatta tgatgttgca aaggctcgcg aaaaggaagt tcgtcacgat gtaatgtcgc
     4621 acgtttacgc atacggcgta cagtgcccgg acgcaaaacc tattattcac ttgggtgcaa
     4681 caagttgtta tgtcggtgac aacactgata ttatcataat gacagaggct atgcagcttg
     4741 ttaagaaaaa gcttgtatgt cttatcggag agcttgcaaa gtttgcaaag gaatataagg
     4801 atttgccgac attggcattt actcattttc agccggcaca gcctacaaca gtaggtaagc
     4861 gtgcgtcatt gtggcttgaa gatttgctta tggaccttga ggacgttgat tatcagcttt
     4921 caaaggcaaa gcttttgggt tgtaaaggta caacgggtac acaggcaagt ttccttgaac
     4981 tttttgaggg tgaccatgaa aaatgtaaaa tgcttgacaa gaagattgcc gaaaaaatgg
     5041 gttatgaaga atcattccct gtaagcggtc agacatatcc gagaaaactt gactctcaaa
     5101 tgcttaatac attgagcctt atcgcacaga gtgcgtacaa gttctcaaac gacatcagac
     5161 ttcttcaaca cttaaagcag attgaagaac cgtttgaaaa gtcgcaaatc ggttcatcgg
     5221 caatggctta taagagaaac cctatgagaa gtgaaagaat cggctctctt gcaagatatg
     5281 taatagtaga cgcacttaat ccggcaatca ctgcatcaac acaatggttt gaaagaacac
     5341 ttgatgacag tgcaaataag cgaatttcag ttcctgaggc ttttcttgca gtagacgcta
     5401 ttctcagctt gtacatcaac gttgttgacg gacttgtggt atatgataaa gtaatcaatc
     5461 agcagtttat gcgtgaaatt ccgtttatgg ctactgaaaa tattatgatg gacgccgtta
     5521 aaaaaggcgg tgacagacag gatttacacg aaaaaatcag agtatattct atggaggcgg
     5581 gaaagactgt taaaatggag ggtaaggaaa acaaccttgc cgaacttatc gccggtgatc
     5641 ctgcattcgg tttgactttg gaagaaatta atgccgttat gaaacctgaa aattttgtcg
     5701 gacgtgctcc gcagcannnn nnnnnnccgc agcaggttga agatttctta aaggattacg
     5761 ttgagcctgt tcttgaaaag aacaaggatt tacttggtat tgaagttgaa attaacgtct
     5821 gatttatttg aaatataaat ttagaggtaa ttatatgaag aaaattatat tttgccttgt
     5881 tgcggtttgt atggttgcga tgtcgtttgt gaccgtacac tgcgaggata atattaaagt
     5941 gatacttgac ggcaaaacac ttgaatttga cgttcagcca caaattatag acgaacgtac
     6001 acttgtgcct atgcgtgtga tattcgaggc attgggtgca gatgttgagt ggaaagagga
     6061 aactcagact atcaacgcaa gacgttcgga tactgatttg ttttatatca gtatgcgtgt
     6121 gggtgacaag ataattcata aggagacggt taaggagaac ttgccgacat caaacgatat
     6181 tgaacttgat gtaccgccga tgattataga cgggcgtacg cttgttcctg ttcgtgcggt
     6241 ttcggagtgc tttggctgta aggtagagtg gaacggcgaa agtcgaactg ttattattga
     6301 aagcaattct gacaataata cagatactga cgtgccgaca ccgtcaccga cagaaacgcc
     6361 tgtcaattca gttaatacaa tcgaatataa cgccgataat gagcagtatg caagttatat
     6421 gaaagaattt aaaattcttg acgcggtaaa aaattcggac ggcaactatg atataacata
     6481 cacacttcaa acatatcttg aaggtcgtgg tttggttgtg gttgaattta ggtgccttga
     6541 tgaaaacggc agtgaagttg acagatttgg cggaacattc caaggcacag attatacatg
     6601 gagcgctcaa gaggcgaaaa caacaatttc cggtaaaaca acacgcattg agcttgtaat
     6661 tgaaaaataa gttatgaaat ttaaaatcag gcaaacgctt ggttagcata ggaggacaaa
     6721 tacatggtaa aagcaatagt tggtgcaaac tggggcgatg aaggtaaggg caagattaca
     6781 gatatgcttg ctgctgacag tgacatcgta atcagatttc agggcggtgc aaacgcaggt
     6841 cacactatta taaacgaata cggaaaattt gcattgcatc ttcttccgtc aggttcattc
     6901 aatcagaatg taacaaatat gatcggaaac ggtgttgcac ttgatattcc tgaacttata
     6961 aaggaaattg acgaggtcgt aaaacgtggt gttccgaagc cgaacattgt tgtgtcagaa
     7021 agaacacagg ttcttatgcc ttatcacaag ttgtttgacc agtacgaaga agaaagactt
     7081 tcagaccgtc agttcggttc tacaaagtca ggtatagcac cgttcttctc agataaattt
     7141 tcaaaaatcg gttttcaggt ttgggaactt ttcgagcctg aaagactaaa ggaaaagatt
     7201 aagaacgtaa tcgaagtaaa aaatcttatg cttgaacata tctatcataa accgctattg
     7261 gacgcagacg agcttttcaa tacacttatg gaatatgccg atatgattaa gccgtatgtg
     7321 attgattcgg ttgcatttat gcacaacgca atcaaagagg gtaagaaaat tcttttggaa
     7381 ggtcagcttg gctcactgaa agaccctgat ttcggtattt atccgatgac aacttcttca
     7441 tctactcttg ccggttacgg tgcggttggt gcaggtattc ctccgtatga aattacagac
     7501 ataattacag ttgtaaaagc atattcatca gcagttggtg caggtgcatt tgtttcggaa
     7561 atatttggcg aagaggcaga tgaacttaga agacgcggcg gtgacggcgg tgaattcggt
     7621 gctacaacag gcagaccgag acgtatgggt tggttcgact gtgttgcgtc acgttacggc
     7681 tgtcgtgtac agggtacaac tcaagttgca tttactgtac ttgacgtact tggttatctt
     7741 gatgaaattc ctgtatgcgt gggatatgaa gttgacggtg aagttatcag agatttccct
     7801 acaacaaaca agcttgaaag agcaaagcct gttttgaaag ttcttccggg ttggaaatct
     7861 gacatcagag gtatcaagaa atatgaggac ttaccggaaa attgccgtaa gtacatcgaa
     7921 tttgttgaaa aagaacttga agtgcctatt aagattgttt ctaacggtcc ttcaagagaa
     7981 gatattatat tgaaataatt aaaatgaacc caagggggaa tttgttatgg acgcatctaa
     8041 aatcaaattg attatatggg accttgacga aaccttttgg aacggtacta tttcagaaca
     8101 aaaggtcgca cctgttaagc aggcttgcga ccttgttttg ttatcctcaa aaaagggaat
     8161 tgtcaattcg atttgttcca aaaatgatga aaagccgtgt atcgataagc tgaaagaatg
     8221 ggatttggat aagtattttg tgttcaattc gattaactgg gagccgaaag gtcagaggat
     8281 taaagatact gtcgaaagta tgaatttaag ac
//
LOCUS       DAWACS010000019         7767 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1058_length_7767_cov_2.647562, whole genome shotgun sequence.
ACCESSION   DAWACS010000019 DAWACS010000000
VERSION     DAWACS010000019.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7767)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7767)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7767
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1058_length_7767_cov_2.647562"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3236..3245
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2577 a         1269 c         1865 g         2046 t
ORIGIN      
        1 tcgtttatga aaacccgaca ccgggtaata aaaaaggcgg tataaccacg cttgaagata
       61 agtcgctcgg ttgcgtgcag aagtctggca gtgcagacgt tgaggacgtt attgaaatcg
      121 gcggaagtgt tacaagaaaa ggacttaatc tgcttaccgg accgggtaac gatattgttg
      181 cggtaacaaa tcttaccgcg tcgggctgcc atttgatact gttttcaaca ggacgcggta
      241 cacctgtggg tgcgcctgtg ccgactgtga aaattgcgac gaatacaaac cttgcaaaaa
      301 gaaaaccaca ttggattgat tttaatgcag gtgagattat aaacggtgcg gatttgaccg
      361 atgaactttt tgaatatgtt atagaagttg caaacggcaa acaaacgaat aatgagaaaa
      421 acggttataa ggaaatttca attttcaaag acggagttgt attatgaaag agtggctgtg
      481 ccgctctttt tttgactaaa tgacgaaaat gtgataaact gaataaaagg ggggagatta
      541 tgaaaagaaa aatttttata ctgacggcac ttgttatgat gatattttgc gtcaatgcgt
      601 gtgcgttttc tgatgtacag agcggaagct ggtattatga caatgtaacc gatatgacaa
      661 accaaggata tttaagcggt tatgaggacg gaacgttcag acctgacgga actgttacaa
      721 aagcggagct tgtatcgata gtcggcagaa ttgcggggct tcaagagagt gcaaagcaaa
      781 acaatcattg ggcagacggt atggtgcaaa cggcacttac aaaaggtttg ttcgattggg
      841 acgagatacc gcctacggca caaacatatg acgagccgat aacaagacag ttggcggtta
      901 aaattgttat gaatgcattt tttaaagacg aacgtggtga ttacaacaga gtgagttcgt
      961 ctgtatctga ttttgcacag cttgacggac gatattatga cagtatgatt gcggcttact
     1021 gcaaaggcat tgtatacggt gatgacaagg gtaatttaaa cccgaagtca tcaattacaa
     1081 gagctgaggc ttgtgcgata attatgcgtg cggcgagtat gaaaggtgat ttaaaacctt
     1141 atgagccgac tgtgacggag caaccaaaac cgcaaacgac aagaaaaggc ggtgtaagtg
     1201 aaaacggtgc gttgcacgtt gacggtacgc aacttatgaa tgaaaacaac gagcctgtcg
     1261 tgcttcacgg tatgtcgtca cacggtttgc aatggtttgg taatttcgcg actgaaaatg
     1321 ctgttaaagc aactgccgat tacggtgcaa acttgttcag atgtgcaatg tataccgatg
     1381 agggcggata tataagcaat ccgtctgtaa aagatacgct tataaatgcg gttgacagtg
     1441 cgatacgtca ggatatgtat gtaataatcg actggcatat actgtcggac ggaaacccta
     1501 tgcaacatat tgatgatgcg gttgatttct ttggtgaaat gtcggagaga tataaggaca
     1561 gcaatgctgt gctttatgaa atatgtaatg agccgaacgg caatgtgact tggaacgata
     1621 atgtaaaacc gtatgccgaa acggtgatac ctgttatacg tgcaaataca aacgctatta
     1681 tattagtagg aagtccgaca tggagtcagg atttgcatga ggctgcaaaa aatccgatta
     1741 atgccgaaaa tataatgtac acatgccatt tttacgcggg aactcacacc gattggctaa
     1801 gacaacgtat agccgactgc ggtttacctg tgtttgtgag cgagtggggt acaagtgcgg
     1861 ccgacggaaa tggcggtgtt tatcttgacg aggcgcaaag gtggattgac tttatgaatg
     1921 aacgaggaat aagttgggcg aattggtcgc tttgcgataa aaatgaatca tctgccgcac
     1981 tggtgaacgg tgcgaatgtg aatgacggaa taagtgagga cgaacttacc gaaagcggaa
     2041 aatttgtttt taaaaatttc tgaaaaaatg tttaaactct taatttggtg gtatatataa
     2101 tacataagca cgaaagagag gggtggaatt tatgaaatac gttgttacga ttgcgagaac
     2161 ttacggcagc ggcggtaagg aaatcggtaa actgcttgcg aaagaactcg gaataaaata
     2221 ttacggtcgt gagatttttg aaatgtcaca aggagttgag agtgatttgg attccggcga
     2281 tgatgaaagt attcataatg catctcttga agaggctgac gaattgttta aacaacagtc
     2341 taaaacaatt cgcgaaattg ctgagaaaga atcgtgcgtt atagtcggac gatgtgccga
     2401 ttatgtgctt cgggatatgg ataatgtcgt taagatttat ctttacgcac caatcagaga
     2461 ttgtatgcgt cgtgtaataa gattgtatga gcttaatccc gaagatgcaa aaagtcttat
     2521 tcattcgatg aacaagacaa gaggtgatta ttatcagcat aataccggtt tgaactggtc
     2581 tgaggctgtg cattatgata tatgtcttaa tacagccgga ttgaaaaaag aaaaggttat
     2641 tgatataatc aaaaactata taaaaacgaa aatagattga tttgaaaaat cctccaaaaa
     2701 agcggtgcta aattaacagc accgcttttt aaatgttata ttttatgctt tgctatgctc
     2761 cgtaccttga ttgcggaata aaagagttat gcaccataga cctgcaagac ctacaataac
     2821 ataaataatt ctgcttatga ttgcggcttg accgccgaag attgacgcga caaggtcaaa
     2881 tttgaaaagt ccgacaagca gccagtttac cgcaccaata attgttatga ttaaagcagt
     2941 attgttcata atacttacac tccttttgaa ttggtttata ttgttgtttt tagtgtaaac
     3001 aaagaagtga aattttaaac gtggtaataa cttcataaaa aatttcatat tatatatggg
     3061 gtgagaagat ggggagaaaa gctatgatgt attttatttc tgttgttata ataatagtaa
     3121 ctacattgat gtttgtgctt tttgccagcg gtgaaaccga tacgaacaat attgtgtttt
     3181 tgaaaggata cggttgggaa gtgaggacaa aatctgttga aaaggaggat attatnnnnn
     3241 nnnnnatata catatatagt agaaaattat ccgaaagatg tcggcgaaga agtcagggcg
     3301 aatgtaatct gtataaataa cgaacctgtc ggcggtgata taatgaccgt aagtatccgc
     3361 ggatttatac attctttgaa ctttgacgat tcacttcgtt aaaatttgat tacaaattat
     3421 taagttttgt aataatacca aaaatcatac gtgtaaaagt atacaaaaaa atatttgcaa
     3481 aatcattaaa tttgtataaa atttaatgat ttttttatac aaaaaagctt gccaaaacgg
     3541 ctgttatagt gtataataac agtgttgtga ctaaagtatg cgaaagcata taggtactga
     3601 caaacgatga agcgggaggt tgcggcgatt taacgtcggt ttttctgtgg agtgagtccg
     3661 attcatgaaa ccgggcggca gtcaatgtat acttaggaaa agtgcggtct tttgacggca
     3721 taagacagga taagtatgcg gcgaaattgg ctaactttgc ccgaactcta caatatgagg
     3781 ttcgggtttt tttataaggg tgatgcgaaa cacccggcgg tgtgtgccat tttctttaag
     3841 cgctgtcgtg cagattacaa cacaataaca attcgaggag ggaaaagtat ggcagcaaac
     3901 cagaaaatca gaatcaagct taaggcttat gaccacgttg tacttgatca gagtgctgaa
     3961 aagattgttg agattgcaaa gagaacaggt gctaaagttt caggccctat tccgcttcct
     4021 acagataagg aagttatcac aatcctaaga gcggttcata agtataagga ttctcgtgag
     4081 cagttcgaaa gaagaactca caaaagattg attgatatag ttgttccggg tgctaagact
     4141 atggaatcac taatcaaaat tgatcttcct gctggagtgg atatcgacgt aatccagaag
     4201 taagatcgtc tataaagtgc agtagcctat gcggctatga tgcaggtcaa gtttccgata
     4261 gcttttgcgg aaaccaataa ctgttaagga ggaaaaatca atggaaaaag caattttggg
     4321 tacaaagatt ggtatgactc aaatctttgg cgaaggcgga gtagttattc ctgtaactgt
     4381 tgttatggca ggcccatgcg tagttacaca gaagaagact gttgaaacag acggctacga
     4441 agctgtacag gttggttttg gtgacgttaa agaaaagcac cttaacaaac cgcaattagg
     4501 tcatttcaaa aaggcagaca ctgcaaacaa aaagtatgtc agagagttca gacttgatga
     4561 ttgcgaaagt ctgaatgttg gtgacgaaat taaggttgac attttcgcag ccggagacaa
     4621 agtagacgta agcggtattt caaagggtaa aggctttgcc ggtccgatga aacacggtct
     4681 acacagaggt cctatgactc acggttcaaa atcaaagaga gtttccggtt caatgggtat
     4741 gtgctcaaac cctggacgag ttttcaaggg taaggtactt ccgggacaca tgggtgttca
     4801 aaaagttaca gtccagaatc ttgaggttgt taaaatcgac gctgaacaga atttaatcct
     4861 tgtaaaaggt gcaattccgg gaaacaaggg cggacttgtt acaatcagaa acagcgtaaa
     4921 ggcataatcg aggttattgg aaaggaggaa atataatggc taaagtggct ttatataaca
     4981 tggaaggcac caagacaggt gatatggaag tatcagacgc tatctttgct gctgaagtga
     5041 ataaatcagt tttgcatcag gtagttgtta actatcttgc aaaccaaaga caaggcacac
     5101 agtctacaaa gacacgtaca gaagttcgtg gcggcggtat taagccttgg agacaaaagg
     5161 gtacaggtcg tgcaagacaa ggtagtatcc gtgctccgca gtggactggc ggtggcgtag
     5221 cacttggtcc aaagccaaga agttacagat tcagcgtaaa caagaagatt aagagaatcg
     5281 ctcttaagag tgctctttca gctaagtatg ctgattacaa aatatttgtt atagatgaac
     5341 ttgctgttga cgaaatcaag actgctaaga tcgtagctct tctaaaggga cttgacgtta
     5401 attcaaaggc tcttatcgtt acagcagatg ctgatgaaaa ggtttataaa tcagcaagaa
     5461 acattaaggg tgttacaccg actcacgttg gtacacttaa cacatatgac gttcttaaca
     5521 acgatgcatt catcgtttca aaggatgcca ttgcaaagat tgaggaggtg cttgcataat
     5581 gaattcattt gatattctaa gaagaccaat catctctgaa cacagcatgg aaactgtttt
     5641 agacagagaa ggtaacgaga ttaagagata tacctttgaa gttccaaaga ctgtgaacaa
     5701 ggttgaaata aagaaagctg ttgaagaagc attcggcgtt aaggttgcta aggtaaacac
     5761 aatgaatgtt cttggtaagg ttaagagaat gggcagaaac gaaggaagac gtccttcatg
     5821 gaaaaaggct atcgttactc ttacagctga cagcaaaact atcgaattct tcgatattta
     5881 atcgttatta atttatagat aaggagagta aaggataatg gctattaaaa agtataatcc
     5941 tacttcgcct gccagaagat ttatgacagt ttcagacttc gctgaaatca caaagaagtc
     6001 accggagcgt tcgcttcttg ctaagaaaga caagcacgcc ggcagaaact catacggtag
     6061 aatcactgtt cgtcatagag gcggcggcaa cagaagaaag tacagaatta ttgactttaa
     6121 gagaaataaa gacggtatgc cggcaacagt tatcggtgtt gaatacgatc cgaacagaag
     6181 tgccaatatc gctcttattc aatatgaaga cggcgaaaag gcttacatca tcgctcctat
     6241 cggtcttaca gacggtgacg tcgtagtatc gggcgaaggt gcagatatta agccgggtaa
     6301 cgcattgttt ataaaagata ttccggttgg tacactaatt cataacattg aattgtatcc
     6361 tggaaagggc gctcagcttg taagaagtgc aggtaacagt gcacagctta tggctaagga
     6421 aggcgactat gcacaggtta gacttccaag cggcgaagtt agaatgatca gacttgattg
     6481 taaagcaact atcggtgttg taggtaacca acagcatgaa aacgtatcta tcggtaaagc
     6541 cggaagaaag cgtcacatgg gttggagacc aacagttcgt ggtgttgtta tgaaccctaa
     6601 cgatcaccct cacggtggtg gtgaaggtaa gtcaccaatc ggtcgtcctg caccggttac
     6661 tccttggggt aaaccagcac ttggtcttaa gacaagaaag actaagaaca gaactgacaa
     6721 gttcatcgtt aagagaagaa acgctaaata atagatattt aattttgata tagattgaaa
     6781 ggaggaagat ttaatgggca gatcacttaa gaaaggacct ttcgttgaag agcgtcttat
     6841 gaagagaatt gacgctatga acgcagctaa tgaaaagaag gtcgtaaaga catggtcaag
     6901 agcttccaca attttcccag aaatggttgg acacacaatc gcagtgcatg atggcagaaa
     6961 gcatgttcct gtattcatca gtgaggacat ggtaggccac agacttggcg aattcgctcc
     7021 tacaagaaca tacaaaggtc atgccggagc taaaacttct aagtaatatc tatgatattg
     7081 cttagaagca aagaaataag atttacagag ggatgttaaa tcccgttact aatattgcaa
     7141 aaggaggaat ccacatggaa gcacgtgcag tgttaagata tgctcgtatt tcacctagaa
     7201 aggttaagat agtgcttgat ctaataagaa acaagcctgc taacgttgca atgggtattc
     7261 taaacaacac acctaaagca gcaagcgagt atttgacaaa gcttcttgcg tcagcgattg
     7321 caaacgcaga gaacaatcat gatatggata agaataagtt atacgttgca gaatgttacg
     7381 caacacaagg tcctacttta aagagaattc agcctcatgc tcaaggcaga gcgttcaaga
     7441 ttttaaagag aaccagccat atcactttag tattaaagga aatggaagac taatcgaaaa
     7501 ggaggttcac tcatgggaca aaaagttaat ccacacggac ttagagtcgg agttataaaa
     7561 gattgggatt ctaagtggat tgcaggtaaa aaagatttcg gagaccagtt ggttgaagat
     7621 tataatataa gaaagttcgt taagaaggct tgctatgatg caggtgttgc taagatcggt
     7681 attgaaagaa agcagaataa agtgtttatt acaattcatg ctgctaagcc tggtatcatc
     7741 atcggtagag gcggtgctga aatcgat
//
LOCUS       DAWACS010000020         7743 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1064_length_7743_cov_2.512487, whole genome shotgun sequence.
ACCESSION   DAWACS010000020 DAWACS010000000
VERSION     DAWACS010000020.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7743)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1064_length_7743_cov_2.512487"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3597..3606
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2307 a         1464 c         2054 g         1908 t
ORIGIN      
        1 ccatcgacaa acggatgatt ttttacaagg ccaaagcata gtcgtgaggc tttatccagt
       61 aaagtaggat atagttcttc tccatcgtat gtttggaatg gtgtgttgat agccgagtca
      121 agaagcgctt catcacgtat atcggatgaa ccgccgaaat tctctataag ctgtgagtgg
      181 agcagtaaaa tctgttcttt agtaagaatt ttcattttgc aagctcctta tataccgtct
      241 tattacgagc gattattttc ttggatacgg ctaatacatc atcagttggc gcagctaatt
      301 gctcatcggc ttgattaaat tctaaaatca agtaacgcgg cgagttgttt ttaagaatta
      361 cagcagagcc gtactggtcg acaagtcttg caactttaga aaaattctgg ttggcttccg
      421 aaatagaaac cattgtgttt gtattaacat tcatatagca tacctccatt tatattctat
      481 aaatattata ctctaatttt aggataaagt caacctaaaa tttattggta aatggtgaga
      541 tttatgaaaa aattaagcat agacattgaa acattttctg acatagacct tatcaagtgc
      601 ggagtgtata aatatgccga ctctcctgct tttgagatac tcctgtttgc ctattccata
      661 gatgacgggg aaataaatat tattgatctt gtaaacggtg aagaactgcc ggaggaaata
      721 gccgaagcaa tcaaatccga cacagttata aagacagcat tcaatgctca gtttgagcgg
      781 gtgtgtctgt cgaagcatct cggcatcctg cttgaccctt cgtcttggta ttgcacagcg
      841 gtacaggctg cggaactgtc gctgccgtca tcgcttgcag atgtcggtgc ggctcttggc
      901 ttggaacggc agaagatgac agaggggaag gagttaatgt ttaaggaata ctgcaaaaga
      961 gatgttgatg ttgagagaca gattgcaaaa aagcttgaga tgtatcccat cagtgagtca
     1021 gagcatcggt tatatgtact cgaccagaga ataaacgacc gtggggtgtt ggtggatttg
     1081 gaattggcgc ggcaggcagt aaagcttaat tccatacaga cagcagtcgc tacggaacag
     1141 gcatatacat tgacaggtct tgaaaatccg aactccgtag cacagcttaa ggcgtggctt
     1201 acagagaacg gtgtggagat tgaaagcctt tcaaaaaagg ctgtggcggc gcttgcggat
     1261 gaaacggacg gcgatataca ggaaatgctg catttgcggc tgttaatgtc gaaaacatcg
     1321 gtaaaaaaat atgaggctgt tatgcgttcg gtatgcagag ataaccgtgt gcgcggaatg
     1381 atgcgtttct gcggcgcttc aagaaccgga cggtggtcag ggaacatact gcagccgcaa
     1441 agcctgcctc aaaaccatct gccggatttg acgcttgcaa gggatattgt aaaagacggt
     1501 gattttgaaa tgcttaatat gacattcggt aatgttccga atgccctgtc agagcttatc
     1561 cgcaccgtcc ttattccaaa accaaatcac agatttatag ttgcggactt ttcagccatt
     1621 gaggcgcgtg tattatcgtg gcttgcaggt gagcagtgga ggcttgacac attcaggaac
     1681 ggcggcgata tttactgcgc ttctgcaagt cagatgttcc gtgttccggt cgaaaaacat
     1741 ggagtaaacg gacatctacg gcaaaagggc aagatatccg aactcgcctg cggttacggc
     1801 ggctcagtcg gcgcattgaa gaatatgggt gcagttgaga tgggagtgcc ggaggacgaa
     1861 ctgcaaggct taatcaacga ttggcgaaat gccaatccgc atatcgttaa gctgtggaca
     1921 gaggtcggaa atacagcgat gaaggcaatt aaggaaaaaa caatcgtttc actcggcaaa
     1981 cttgtattta tgtacgagcg agggatatta tttatacgcc tgccgagcgg acgcagactg
     2041 tcatacataa agcctcgtat cggtacaaac cgatttggcg gcgacagtat cacatatatg
     2101 ggcgtcggcg cgtcaaagaa atgggaacgg cttgaaacct tcggcggtaa gctggtagag
     2161 aatatcgtac aggcgattgc gagggacttg cttgcttcgg caatgatgaa tgtcgcaaat
     2221 gccggctaca atattgtttt ccatgtccac gatgaaataa tagccgaagc gccggacggt
     2281 caaggctcgg ttgatgagat gtgtatgctg atgagcataa atcctgattg ggcggacggt
     2341 attcccttga gcgctgacgg ctatgagtgt gaatattaca ggaaggatta aggtttatga
     2401 aaaagaaagt aattttaacc gctgtcataa tcggcgcact gtccgagctt gcagcggtta
     2461 tatatacaat acatatacgc agcaatgccg taatcggtgg agaatatttt attctgccgc
     2521 tctttattat ggcggcatac tgcgttttag agataatcgg cacggtagag agtaataaga
     2581 aaacagcggt aagactgaca aggcggacga agtatggaga tgcggtttta aaccatgagg
     2641 tatttcccga atatgcagag gaaactctga tacatgaggt gaattgcttt gaaccgatga
     2701 aaacagcagt cgagaaattg tgcgagtacg aagaaatggg aggaaattaa tatgcttaca
     2761 cgaataccgt gtacggacaa cacagactgc tttgcaaata atgacggcta ctgtgtttgt
     2821 ctgatgagta atgattttaa cggacgaaag tgtccgtttt acaaggagaa aactataact
     2881 gagacagaat gtacactgtc agaggtcagg ctgctcagaa taggcagaaa ggatttgata
     2941 gaaatgtatc tcaggaggat ggtcgatgta cagaaataat gaacattatc ccgacccgac
     3001 cttcgcgtcc gcttatgcgg cgataaagaa agaaattgca cagaataacg gcgaaacgta
     3061 tatgccttgg gtttacatag catcaccgct ccgtggggat atagagggca acatcaaaag
     3121 agcgaaggaa tactgccact ttgccataaa gcaaaacgcg gtgccgatgt gtccgcatat
     3181 atacttcccc ttattctttg acgacaacgc ggagcttgag cggcggatag gtttaatcct
     3241 cggtttgcag atgcttaaac gctgcaagga aatgtgggtg ttcggcgaca ggatttccaa
     3301 gggtatggcg aatgaaatca aaatcgccga gaagcgtaaa atccctgtca ggtattttac
     3361 atcggaatgt gtggaggtga atacgctatg aaaatatcag ttggaaacag cagaacgtca
     3421 agggcgtgga agataaaaga aatgtcgtgg gacgagttta cggagagatg ttcgcagaca
     3481 atccgaacgg cggaaaccgt gcaggaatat cgaaagctcc cgaaaggaca gcaggacaat
     3541 atcaaagatg tgggcggctt tgtcggtggc gagcttagaa acggtatccg taagaannnn
     3601 nnnnnnggat accgttgtaa accgctgtct gctcacgctt gacgctgact acgcagacga
     3661 ggatttttgg gaacagatag agctgttttt tgacttccaa tgcctgatat actcaacgca
     3721 taagcataca ccggagagac cgagacttag gcttataata cctctgtccc gccctgttac
     3781 ggcggacgag tacacggcta ttgcaagacg catcgccgcc gacatcggaa tagagcagtt
     3841 tgacgatacg acctatcagc cacaccgtct tatgtattgg gcgtcaacat ctgctgacgg
     3901 tgaattcgta ttcaagcatc gtgacggaaa tcctgtagat gctgatgatg tgcttgcaag
     3961 gtataaggat tggcacgata catcggaata tcccgtatcg tcaagacaga aaaagatagt
     4021 atcccatgca ttgaaaaagc aagccgaccc gctgataaag gacggcatag tcggcgcgtt
     4081 ctgccgcgcg tatccgatac agcaggcaat aagcacattt ttgtcggatg tatatacgcc
     4141 gagcagctta agcggacgtt atgactatat cccggcagac tcaatcgcag gggttatagt
     4201 ctataacgat aaattcgcat acagccatca cgcgaccgat ccggcttgcg gtaagctgtg
     4261 caatgcgttt gaccttgtgc ggatacataa gttttcgtat cttgataagg acgaaactga
     4321 cagtgagaaa tcaccatcct tcaaagcaat gatggacttt gcgatgaatg atgacaatgt
     4381 caaagcacag acgcttactg ataaggaaac ggcggcgcag gaggagtttt cggctatctc
     4441 ggacgatgac gatgtaatgt ggcagcacag gctgtctatg aacaagcgag gagatataga
     4501 gaacactata caaaacctca cgattatatt gcagaacgat ccgaagctta aaggtatcgt
     4561 tttcaacgaa ctgtcggatt gtatagagat aaaaggcagt gtgccgtggc agcatccgtc
     4621 taaatattgg cgcgatgcgg atgacgcaca attactcaca tacttaacct accactacgg
     4681 taaattcaca agggtgaact atgacgtggc attggcaaaa gtagtcgatg accgctcgtt
     4741 ccatcctatc cgtcagtatc ttgacggctt gccggaatgg gataggacgg agcgcgtgga
     4801 tacgctttta accgattacc tcggcgcgga ggataatccg tacacacgcg ccgtaatccg
     4861 taaaacctta tgcggtgcga tagcccgtgt tatgataccg ggcattaagt ttgatacgat
     4921 gcttgtattg tcgggaccgc aggggattgg taaaagcacg attatatcaa agctttgcgg
     4981 tgagtggttt aacgattcgc ttttattatc cgatacgaag gataagacag cggcggagaa
     5041 actacaaggg ttttggattt tagagatagg cgagcttgcc ggactgaaaa agaccgaaat
     5101 cgaaacctta agaggtttta tctctcggca gaacgatgtg ttccgtgcgt ccttcggtcg
     5161 cagagcgact ccgcatttaa gacagtgtat tttcataggc acaacgaatg cggaacacgg
     5221 atatctcaga gataccacgg gcaaccgacg tttttttcct gttaaggtat cggggaactc
     5281 ggagaaaaag ccatggcagc ttacgcagaa tgaaatagac cagatttggg cggaggcgct
     5341 tgtgtattac aggaacaacg aaccgcttat tcttgacgcg gaaaccgagc ggtatgcgaa
     5401 gaaccttcag cgcgaggcta tggaaaccga cgaacgagag ggaatggtac gggagtacct
     5461 tgaaacgttg ctgccggata attggaatga gatgtccttg tatgaacggc gaaatttcct
     5521 taacggcagt gagtttggag aggtgtccgc aaaaggcacg gtgcgccgtc agaaggtttg
     5581 caatatggaa atatggtgtg agtgcttcgg aaagaacaag gcggatttga aacgccttga
     5641 cgcaaatgct atctcggcta ttatggctaa gatggaaggc tggcagaaat ccggcaaaaa
     5701 gtcgaggttc ggtatgtacg gaattgtcgc gggctatgaa cggatatagg gtagaaatgt
     5761 atgcgattgt gtttccgagg ttgctttgga ttgccataaa aatagtgcgt aagcaacttc
     5821 ggaaaacggc ataaaatcaa ggttactgac tatgtttgtt gctttgtttc tgaaaaattc
     5881 tatatagatt atatattata tataaaaata ttacacacac atgtatatac gcgcacgagg
     5941 gatttttgag caaatggcaa caacatcgga aaatatggag gaaaaatgcg cgaaaaggat
     6001 atagagcgaa agcttgtaaa cgcagtaaaa atgcgaggtg gcatggcttt gaagtttgta
     6061 tcgccgaata tgaatggcgt tccagataga ttgttactgt tgccaatggg taaggcagca
     6121 tttgcggaac ttaaggcccc aggtaagaaa atgcgagcct tgcagataaa gcgaaaaagg
     6181 caattagaag cattagggtt ttcggtgtat tgcattgacg gcatagagca aatcggaggg
     6241 gtgcttgatg aaattgaaca gagatgattt gcatgagttt cagaactacg gtgttgactt
     6301 tataataaat aaaccgattt cagcattgat gctcgaatgc ggtcttggga aaacaataac
     6361 cacgcttacc gctgtcagtg atttgatata tgactacttt gagatttcaa aggtgcttat
     6421 aatcgcgcct atgagagtag ctctcacagt atggaagcag gaatgcgata aatgggaaca
     6481 gctaaaatat ctcagatgct caatcgctgt cggcagtgtg tctgaaagag aaaaagccct
     6541 gcaatcagat gcggacatat atatcatcaa ccgcgagaat gtggaatggc ttgtaaagaa
     6601 ttatccgttt gattttgata tggtagtcgt tgatgagctt tcgagtttta agtcgcatcg
     6661 gagtaagagg ttcagagcat taaggaaggt acgaccgaag tttgacagga ttgtcgggtt
     6721 aacgggaaca cctgcgccta acgggttaat ggatttatgg gcggagataa acctgattga
     6781 tatgggagag aggcttggaa gatatataac tcggtatcgt gatgagtatt ttaagccgga
     6841 taagcgtaac ggcgcaatcg tgtattcata taagccattg cctgatgcag aggagaggat
     6901 atacgggaaa atatccgaca tctgtgtttc gatgaaagca gcggactatc ttgaaatgcc
     6961 ggagagggtt gataatattg ttgaagtcgg tatgagcgat aaggaaacgg caatgtataa
     7021 acggcttgaa aaggagatgc tattgccgtt tgccgatggc gatattgacg cggtaaatgc
     7081 ggcgagtctt agcaacaagc tccttcagct tgccaacggt gcggtctatg atgagaacgg
     7141 aaaggtcaag aagatacata gccgtaagct tgacgcttta gagggcttaa tcgaagcggc
     7201 aaacggaaag tctgtgcttg tgtattacag ctacaagcac gacagggata ggatttcaga
     7261 gcggttcgat gtccgtgaga taaaggacga taaggatata tcggaatgga atgcaggaaa
     7321 gatacagctt gccatagcgc atccggcaag ctgcggtcac ggtcttaatc tgcaaagcgg
     7381 aggcagcacg attatatggt ttggactgac ttggagtttg gaactgtacc aacagtcaaa
     7441 cgcaaggctt tacatacagg gacagaaaaa cacagtggtg attcatcaca tcattaccaa
     7501 aggcacggtt gatgaaaagg tgatgacagc cttaaagaat aaagatatag gacaggcttc
     7561 tttgatggag gctataaaag cgaggataga ggaggtaagg gattgatgac ggcaaaggaa
     7621 tacttaaatc agcttatcgc aatggataac gcgattaaca ggaaacagca gcgtcttgcg
     7681 acacttcgtg atgtggcgat gaacacaact ccgaactatg ctgacgaggc tgttcagcgt
     7741 aca
//
LOCUS       DAWACS010000021         7179 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1183_length_7179_cov_2.456064, whole genome shotgun sequence.
ACCESSION   DAWACS010000021 DAWACS010000000
VERSION     DAWACS010000021.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7179)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7179)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7179
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1183_length_7179_cov_2.456064"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2228 a         1591 c         1371 g         1989 t
ORIGIN      
        1 gaacacctgc aatactgcat tgcactcttt atcctgacgc gacaaattag tacagcttcc
       61 cgttacatta aagaatacag tcggtttccc ttgtgcaata actgcatcat aaagcctcat
      121 ttgacacgga ggcagttcta aatcacgctt gtcgccgctg taatcggcat tatacgcatc
      181 tccagcctct ccttcaagca aagtattaag ccccatacaa aggattacca catctgcatt
      241 ccttgctgct ataattgcct cctcaaacgg aatatgcggt gagtttgagt ttgatatatc
      301 acagcctttt gcatatgtaa tatgcccgct tgctttatct tgtattcctt tgagtatggt
      361 tgaatacctc gatggtgttc cgttataatt gccaagaagc acagaaacgt catctgcatt
      421 aggtcctatt accgcaatgt gttctgttcc tttaagcgga agtattccgt catttttaag
      481 aagaacaacg ctttcctgcg ccatcttaag atttgtttcc atatgctcct tacattctat
      541 gacatcaagc ggaatgtcgt tgtactcaca gtcatcatca aacattccca gtctatacct
      601 tgcttcaaac agcttttcca ccgcttttgt tatggtttcc tcgctcacaa gtcctcttgc
      661 tatggcggct atcaaataag catattcagc tccgcagttc attatacagc cattgtttac
      721 agcaagcgca gcactctcag ctgcatcttc tgtaactctg tgatgtgtat gaaaatcaca
      781 aacagctccg caatctgata caatatatcc gtcaaacccc cactcatcac agagaatatc
      841 ctgaagcaat gttttactgc cgcagcaagg ctcaccgttt agtcggttgt atgcgcccat
      901 aactgccgca ggctctgcgt tatcaataca atatttaaac gcccacagat aagtttcacg
      961 caaatccttt tcgctgacaa cagaattaaa actgtgtctt attccctcag gtccactgtg
     1021 aacagcgaag tgcttcaatg tcgcatctgt ttttctgtac ttgccatcac cttgcattcc
     1081 ttttacaaat gccgtaccaa gcactcctgc aagatacggg tcttccccgt aggtttcatg
     1141 acccctgccc caacgcggat ctctgaaaat atttatgttc ggcgcccagc aggtaagtcc
     1201 ctgatatatt tttgttgatc caaatgattt gtatccgtta tatttggcac gcacctcatt
     1261 tgaaatatcc tcagccacct tatacatcaa atcttcattg aatgatgctg ccattgcaat
     1321 tgcctgtgga aaaacagtcg cagttccact gcgcgcaacg ccatacaaag cctcattcca
     1381 ccaattatat gccggaattc caagacgttc tattgccggc gcgtcatgct gcatttgtgc
     1441 tattttttca gccaatgtca tttttgacac aagctctttt gcccgttcct taaaatcctc
     1501 cattatattt acctccctga acagagttct cataattcca ctgttttcaa ttttttataa
     1561 atacagttta tcatatgttt ttataacatt caatactttt ttcttgactt tgttatgttt
     1621 ttattataga attaaattat caattgttat acttattata tgtattcgaa tataatacaa
     1681 actctttttg ctggaggttt tcaatatgaa tttatcatca aaattgcttc gcatatgcac
     1741 tggtgttcag gctgctgcca taattgccat ttcaatgcct gtttcaacat cctatgccgc
     1801 cgcacctgat ccgaattggc atgtagtaaa aacacaatat aaaacagacg attatgttat
     1861 agccggatat gaagccaagg attttggaat ttccgctaac ggcagaactg atgtaactga
     1921 aaaaatccag accgctcttg acgaattata caaaatcgga ggcggcacgt tatttttacc
     1981 tgcaggacgc tatgttgtaa agggaacact gaaaatacct cagggagtaa ttcttgccgg
     2041 cgactggcaa agtcctgacg aaaacaacgg aaaggctgcc ggaacaatac ttatggcata
     2101 ctcaggacgc ggagaaaatg atataaatga caattacgac agtgagactg aaaatatgcc
     2161 gtttataaca ctcgaaccaa acgcatccat caagggaata aatatatggt atcccgaaca
     2221 ggcacctgac aatattgtcc cctacccgac gactattcgt atgtacgacc ctaaaacatg
     2281 gggcgcggac agcacgagaa taagcaatgt tacatttgtg aactcataca acgctattcg
     2341 tcagggaccc tattcaagcg gttgtcccaa tattgaaaat gtatacatat cgcccttgca
     2401 caccgcagtt gatattgacg gtcttgctga tgttggtcgt ttcactaata ttcatatctc
     2461 gcccgattat tggattaact ccgaacttga taacgccaaa gagtgtgaaa attctctgcg
     2521 aacgtataca aaggaaaacg caaccggtat caagctcggg cgaatagact ggtcatatct
     2581 gtctttttct gaaattgaag gctgtaagca tggtatggag tttacacttt ctcacagcgt
     2641 tctaagctac aaagacggag atcttaacgg tactatgaac gcatggtcgt atcctaacgg
     2701 acagatatac aatgcagata tatccggctg tgaagtagga ttattctcag aaggaacctc
     2761 cggcgcgggt atgctcgttg ctgaaatgaa tatatatgac tgtgacacag gaattgaagc
     2821 tgtaaacggc atcgaatcag gcaacggcaa tcttgaaata gcaaactctg ttataagcgg
     2881 cagcaattat gcaatcaact ataacgctct ttcgagaatg tgtataggaa attcaaagat
     2941 tgacggaaac atagatattc aaagcagcaa tgtttctatc gcaggcgcgg aatttaagaa
     3001 ctgcccttct attacgctga aaaacaacac tgatacagtt cttgataaaa atggaaatcc
     3061 cattataaaa acaggacagc tgattatttc ttcatcagat aatcttcctg ccatagataa
     3121 tcaaaacagc agtcaggtta tacataatac atcgcaatat atgcctccaa atgtaagcag
     3181 cattacagaa gagcccaacc ttgtcacaaa accgtataag cctgatatat tcgttgtaac
     3241 caatgcgccg tataacgcgc cgcacaacga ggttgacttt caaaaattca actctccatg
     3301 tgaacaggac gcatcagcag caatacaaca ggctcttgat gacgccgaag caaacggcgg
     3361 aggtattgta ttctgtccac ccggcagata tacattaaag tcaccccttt cagttgcagc
     3421 cggtgttgaa ctcagaggcg caatagacca tacgcgctta ccgataaagc tcggaactat
     3481 atttgatacc gttgttcccg aaaatgaatt tttaaatacc tccttcacgg aacttaacac
     3541 aaaaaccgtt gacggaattg ataatgtctt aagcggcaac agtatttttg gagaagtgcc
     3601 ctctcccatt gtaaacgaag acggtacagc tgcacgttct gtaacagtca caccctacgg
     3661 cttacgattg tattcagctg cagccgcaga acaggctcac gcatttgacc attcaactca
     3721 gcttgaatgc gggaaaagcg acagtaaata tatcattgag gctgttgtgt ctccaaaatc
     3781 cattaaagag aacggcgaat tttttataac actttcaaga catgatgcag gaacaaataa
     3841 aaatgcggat gcttttcgta tagccaattt caaggctaac ggtaatatcc ttgagctact
     3901 aggcggcgta aaaactgaat ttttgaatga tgaaatgtat tctttccgat gggaacttga
     3961 ccttacagat ataaacaata aaacaatcac aaccaccgta atagccacaa agggcaacca
     4021 aaagggcgag atattcaagt ctgccgctaa atttccggat gcctatcaag gaaatgtcga
     4081 taatgtaggc cacatacttc tgcaagcctc taaagtttca ggcgagagca gcggtgaatc
     4141 agaagtatat gttcatggcg caagcgtaca aagggacaat gagctaaatg aacctgcatg
     4201 ggttatgctc agaggcggca gcggtataaa tggtattaca tttaactgta taggtcagaa
     4261 atatcttccc tcacccaatc cctctcccta tatgattcgg ggacaaggca ataatatata
     4321 tgttaaaaac atcgctcttc gcaatgtgta tgacggcata gatttcaaga cctattcctg
     4381 caataaccat tatataaacg gggttgcggg cattgcgtgg cacagtgaaa tagatattgc
     4441 caattccgat aacggcgtta ttgcaaatac tcaatttaac tggggttctc tgctgtacgg
     4501 ttcgcaaaac aagctcggct gctgggaaga ctcacctatt aacagctata cagacgcaaa
     4561 cggcagcaat atagacccgc gcagctcagt aaatgaaccg tatatggaaa aatatatgtc
     4621 aatgaacctt gattgtgtca cggttgacaa ttgtaaaaac atactgcttt acaataattt
     4681 cagcattttc ggttcaagct cactcacctt gaaaggtgat acaaagggac tttgtatagg
     4741 tcagggcgcc gacggctctc gttttggact gagagttgca gacaatgcca atgtggattt
     4801 tgtcaatacg cagatagtaa gcttttatta taactccacc tttaacggtg taagcagaac
     4861 tgcttttgcg gatttgagca atgacgcata tgtctttttg caaaccggat ggataaacaa
     4921 cgccaacgca aacactgtgc agcctgtaat cggtgagaat aatatttcct ttagggattt
     4981 gttttttaac agaaactata acagcctgcc agaaacaaca aaacagccat cagacaaacc
     5041 aaattccgag ccgagagcac cgctcaatcc aagacccggt acatattatc cgctaaactc
     5101 caaaccgaca tatatcgccg agcttaacga aacaacagaa tttaatcgca tacagcttgt
     5161 gtgggaggag gaacaaagcg atgatgttcg tgtcaatgta aacggaatca ttttagaagg
     5221 aactgtaaac ggaaagacac tcaacctttt aaacggagcg gctgaggcgt cggaaaataa
     5281 ttctaattcg gaggtgttta atctttacaa tctcacagcg gatcatattg aaattacggc
     5341 agaaaacaag ccgattgtaa agctatacaa ggatgaaaac ttgcaatata aaatcactgc
     5401 aacaggctca gagcttttaa gcagccctga atatatgttt gacggaaata aagcaagcga
     5461 cagcaatttc tcaatgttct ggcatgttga cccaaaagca acgccttctt caagctttgt
     5521 ttcggcatct tttgataagg aatacagtat ttctcagctt gcgctgtacg catggggacc
     5581 taacggctgg tatcctaaag gctttgatat atccactcat tcgttcactg cgcaaagtcc
     5641 tgcggaaata ggctatccgc gcagtctgcc ttatgtgctg tcatcaacgc ttgatataac
     5701 agcagatgaa tttacaatca ctttccccga aggcggctac agcgatataa acaatcctga
     5761 cacaaaagag gatggctcgg ttcagtacaa atatggtttc cgtttgtttg aacttgaact
     5821 gtatggatca gtagattctg tatggtcagg agatgtatcc gttatcccgt ctgaaaagcc
     5881 tgtaacaatt cctgagatca ctaatactcc agagcctaca aatactccag agtccacaag
     5941 tatacctgaa cctacaaata ctccggatat cccgcataca acagatactc ctgctcctga
     6001 taaagatttt ttagctttaa ctgcgacaga gtcggatagc ggacttacat ttaccgcaac
     6061 agcaatgctt gcacagcctt ttcccaatcc atatacagca atacttgcac tgtatgataa
     6121 ttcgggtgtc cttataagcg tgcgaatgca ttcggttaaa gaatcccctg aagaaaacac
     6181 tttaatattc gaaaacatta ctgttcccaa catagaatat tacaaagtcg cattattcag
     6241 atggaatgat atagatgggg aattcggaat gatgcccgtt gctgaaccta tattttccgc
     6301 ttatacatcg actaagaccc aataatcata aactgacttt gggagactcg acgctattgc
     6361 tcgggtctct ttttttcatt tctgctgtat ttttaactga caaattcatt taattgtatt
     6421 cattataacc aaatacgctt atttgtcata tttttctcaa aaaaagattg atttttatat
     6481 attaaagtgt tataattgtt atatcattat taattaaatt gatatatctt taatcagaaa
     6541 ggaattaaga ttttatgaca aaattatcgc ccataaaact aacacccgcc tacaaagact
     6601 atatttgggg cggaacaaag cttaaaacaa tgtataacaa acattccgcc cttgatattg
     6661 tcgctgaaag ctgggaactt tcagcgcaca aggatggtga aagcatagtt gcagacggtc
     6721 catatagcgg ccttacgcta acacaatacc ttaacgcaat aggaaacagc gcacttggca
     6781 caaatgctaa aaactttgat tactttccta tcctaataaa actcatagac gcccgcagtg
     6841 ctctttccgt gcaagtacac cctgatgaca gctatgcgct tgagcatgaa ggcgagtacg
     6901 ggaaaacaga aatgtggtac atactcgact gtgaagaaaa tgcatcgctg tattatggct
     6961 ttaaaaagga cgtaacaaaa gaagaataca agaacgcgat tgagaataac acacttacgg
     7021 atatattaaa caagattccc gtacacaagg gcgatgtatt tttcattcct gctggaacag
     7081 tacacgcaat aggcgccgga atactgatat gtgaaataca gcagaattcc aatactacct
     7141 accgtgtata tgactataac cgacgtgata aaaaaggaa
//
LOCUS       DAWACS010000022         7083 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1199_length_7083_cov_2.885174, whole genome shotgun sequence.
ACCESSION   DAWACS010000022 DAWACS010000000
VERSION     DAWACS010000022.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7083)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7083)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7083
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1199_length_7083_cov_2.885174"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    375..384
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5867..5876
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2108 a         1539 c         1258 g         2158 t
ORIGIN      
        1 atgcattaga atacgaaata gtagatagaa attatgctcg aacttttaat acttcatccg
       61 atgttttaaa agaatgtgaa gaaaactccg acagtcatat aacattttcg gacgaagaaa
      121 tatcaatatt gtgggataat ctttataaag ttccatatgt cgatattttg ttaatacaat
      181 gctattctgg atggcgaccg caagaactcg gtttaattaa actaaaaaat atagacttta
      241 aaaacaatac ttttattggt ggaatgaaaa ccgaagccgg aacagaaaga attgttccga
      301 tacattcaaa aataaaagag cttgttaaat ttagatatga cgaagcagtt agactgcata
      361 gtaaatatct ttttnnnnnn nnnnacaaat atagaagccg tttcaaaaaa attatagaat
      421 gtttaaattt aaaccctgaa cacaaaccac atgacggtag agtatatttt gtcacaatag
      481 caaagaaata taacgttgat caatacgcta taaaatatat agtcgggcat aaaatcaatg
      541 atataacaga aaaaatatat actaagcgag aaatatcctg gttaaaatca gaaatagaaa
      601 aaattaactg atttacagtt ttagttgtgt aggaatttaa gtataggagt agtgtaggag
      661 taatgtagga attacgtaca ttactccact tttttcaact ttttaccact ccttaaacac
      721 gcatgtttgc tatgtttatt agaacttacc ggcctttgca gcttcctcta cggcaacggc
      781 aacagcaact gttgcgccaa ccatagggtt gttacccata ccgataagac ccatcatttc
      841 aacgtgagca ggaactgaag atgaacctgc gaattgagcg tctgagtgca tacgacccat
      901 tgtatctgtc ataccgtatg aagcaggacc tgcagccata ttatcagggt gaagtgtacg
      961 acctgtaccg ccgcctgatg caactgagaa atacttctta ccttgttcga tacattcttt
     1021 cttatatgta cctgcaacag ggtgttggaa tcttgttggg ttagttgagt tacctgtgat
     1081 agaaacgtca acgccctcgt ggtgcattat agcaacacct tcacgaacat cgtctgcgcc
     1141 gtagcaacga acgtttgctc tttcgccctt tgagtaagct gtttccttaa cgatttcaag
     1201 cttacccgta tagtaatcaa acttagtctg tacataagta aagccgttaa ttcttgagat
     1261 gatttgagcg gcgtccttac caagaccgtt aaggataact cttaggtctt ccttacgagc
     1321 cttgtttgca cttcttgcaa gaccgatagc accctcagca gctgcgaatg attcgtgacc
     1381 tgcaaggaat gcgaaacatt ttgtttcttc tcttagtagc attgcgccaa gattaccgtg
     1441 tccgatacca acctttctgt cgtcggcaac agaaccagga atacagaatg actgcaagcc
     1501 aagaccgatt ttctcggcag cctctgcagc tgtcttaaca ccgtctttaa ttgcgatagc
     1561 cgcacctact gtgtaagccc aacaagcgtt ttcaaagcag attggctgaa tactctttgt
     1621 gatgttgtat gcgtcaacac cagcgtcatc acagactttc tttgcttctt ctattgatga
     1681 aatgccatgc tttttaagct cagcattgat ttgatctatt cttctttcat aactctcaaa
     1741 taaagccatt atatatttac ctcccttttc agattattcg tgtcttgggt caagaagttt
     1801 tgcagcatcg tcaactcttc cgtattgtcc gcttgccttt tccaaagctt cgttagcgtc
     1861 cataccctta gctatcattt ccatcatctt accaagatga acaaacttat aaccgataat
     1921 ttcgttgtta tcatcaactg caatctttgt tatgtaacct tctgcaagtt caaggtatct
     1981 tgggcccttt tcaagtgttg cataagttgt accaacctgt gatctaagtc ccttaccaag
     2041 gtcctcaagg cctgcaccga ttggaagacc gtcctctgaa aaagctgttt gtgtacgacc
     2101 gtaaacaatc tgcaagaaaa gctctctcat agctgtattg atagcatcac agacaaggtc
     2161 tgtattaaga gcctcaagaa ttgtcttgcc tacaagaatt tctgatgcca tagcagctga
     2221 gtgtgtcata cccgaacatc cgactgtttc taccaatgcc tcttggataa taccttcttt
     2281 tacgtttagt gtaagtttac aagtaccctg ttgaggtgca caccaaccaa caccgtgtgt
     2341 aagaccggaa atatctttaa tttcctttgc ctgtgtccat ttaccctctt gagggattgg
     2401 agcaggaccg tgatatgctc cttttgtcaa aggacacata tgttctactt ctgctgaata
     2461 tttcatacta ttactccttt cgatttatgc ggcaaaatcc gctcattatc ttcattatat
     2521 catattaatt tcattgtatc aataaaaaaa tatacaaaat tatcgtttat tgtaactttc
     2581 tttatacata aaaacatcat atctcaaata ttatcgtttt actattgccc aaattcggat
     2641 aaaatgttat aatgttagcg taatttttaa aatatatttt ctcacagaaa ggctaaataa
     2701 ttatgaacgg taatgtaatt gtaggacaat cgggcggacc gacatctgta atcaattcaa
     2761 gtcttgtcgg agtttttcaa ggcgctaagc gtgccggctg cggtaagatt tacggtatgc
     2821 gaaacggtct tgagggactt ttgcaggaga gatatgtcga gcttaacgac tgtatcaaaa
     2881 acgaccttga tattgaaatt ttaaaacgca ctccttcatc atttctcggc acttgccgtt
     2941 acaagttgcc acattatgaa aaagacagtg ccgtatacga aaaacttttt gcgatactta
     3001 aaaagcttga tattaagcac ttcttctata tcggcggcaa cgattctatg gatacgataa
     3061 aaaagcttgc cgaatacgca aacaagataa acagcgatat tacatttatg ggcgtgccga
     3121 aaacaatcga caacgacctt gacaaaaccg accatacacc gggttacgga agtgcggcaa
     3181 aatacgttgc gtccgcaatg aaagaaatca tccttgacac cgacacttac acgaccgact
     3241 caatcacaat agttgaaatc atgggcagaa acgccggttg gcttacagcc tcgtctgcac
     3301 ttgcaagaac ggaggactgt acaggtcccg accttattta tcttcccgaa acacctttca
     3361 gctacgataa atttgtaagc gatattttag aggttaaaaa gcataagtcg tctgctatta
     3421 tcgcattgtc ggaaggtata cgaaacgccg acggtcaata tatatgcgaa acacaaaagg
     3481 acgatatgaa acttgacgta ttcggtcaca aaatgcttgg cgaaaccgca cttttccttt
     3541 ctgatatggt tggtaaagat accggtatga agtcaagagg tatcgtgttc tcaacacttc
     3601 aaagatgtgc gtcacatatc gtttcaagac gcgatataac agaggcatac gacgcaggtg
     3661 caacagccgt tgcggcagcg cttgcaggcg aaacaggtaa aatggttata tttaaacgta
     3721 tatcaaacga gccgtataaa gtgtttaccg aaactcacga catcggcgat attgcaaatg
     3781 tagaaaagac tgtgcctaag gaatggatta cccaaaacgg cacctatgtt tcacaagaat
     3841 ttatagacta cgcacgtccg cttattatcg gtgaacttac accgtttatg gttgacggtc
     3901 tgccacgtca tttgacaata gactaaaaag tcttgttgac tgccaaatac aatgtaattt
     3961 taaagagctt attcaaaaat gaataagctc ttttttcaca ctttctccaa tttacccgct
     4021 atttcacaaa catacaaaaa gcaagccgaa aaatcggctt gcttttagaa ttacatattt
     4081 tccattgact taatattttc aaggactttt tcaagcattt ctttatactt ttcgccctta
     4141 gctttttctt cgtcaacgac cttttgaggt gcttttgaaa cgaaaccggc attattaagt
     4201 ttgccttcta cacgtttaat ttcgccctca agctttttct tttccgcatt aagtctttca
     4261 agttcctttg tcttatcaac aagttcatca agcggcaaaa atacttccgc tgacggaaca
     4321 accacgttta cggcgttatc gctgataccg ctcttatccg tctgaacaac agcctcgctt
     4381 gcagacgcaa gtttttcaaa gaatgctgta ccctgtttga acacgtcagt tttatcagtt
     4441 acgataataa cctttgcttt ctttgacggc ggaacattca tttcagctct tctgtttctg
     4501 atagcactga ttgactgcat aataagttcc atgctctcaa cgtccttagg gaagttaagt
     4561 ttttcgtcat attcaggcca ctcacttatc ataatgcttt cgccctcatg aggtagatgc
     4621 tgccaaattt cttcagtaat aaacggcata aacgggtgaa gtaacttcat tgtatttgaa
     4681 agtacgtatg tcaatacata ctgcgccgtc ttacctgtcg gattttcctt atcgaacaat
     4741 cttggcttta caagctcgat ataccagtca cagaagttct cccaaatgaa atcgtacaag
     4801 tttgaaagtg cgataccaag ttcaaactta tcaagatttt cagtaaccga cttaacaacg
     4861 tcattatact ttgaaagtat ccatttatcc tcaagctgta aatcgttcaa atcaggtaat
     4921 ttattttcag ttacatcaag gttcataaga gtaaatcttg acgcattcca aatcttattt
     4981 gcaaagttac ggctcgcctc gacacgttca atatagaaac gcatatcgtt acccggtgca
     5041 ttacctgttg caagagtgaa acgcaatgcg tccgcaccgt attggtcaac aatttcaagc
     5101 ggatcgatac cgttaccaag cgacttactc atttttctgc cctggctgtc acgaacaaga
     5161 ccgtgaatga acacatgctt aaacggtact tcacctgtgt gttcaagtgc cgagaacatc
     5221 attctcatta cccagaacgg aattatatcg taacccgtta caagcacgct tgtcggatag
     5281 aagtaatcaa gttcttctgt cttatcaggc cagccaagtg ttgagaacgg ccacaatgcc
     5341 gatgagaacc aagtatcaag agtatcgggg tcttgacgca ttttctggcc gcacttaggg
     5401 caaactgcac tgtcctcttt tgtaacaaca atttcgccgc aatcatcaca atagaaagca
     5461 ggtatcctgt gtccccacca aagctgacgt gaaatacacc aatcacgaat attttcaagc
     5521 cagtggaaat acaccttttc aaaatgttcg ggaacaaact gtgtatcgcc gtttttaact
     5581 gcatcaatag caggctgtgc aagaggcttc atttttacaa accactgctt tgaaacgata
     5641 ggctcaacag ttgtaccgca acggtaacac tgacctacgt tatggctgtg atcctcaacc
     5701 tttacaagaa gtccttcgtc ctcaaggtcc ttaatcattg ccttacgaca ctcatatctg
     5761 tccataccct catacttgcc ggcatatgaa ttcatcgttg cgtcatcatt cataacctta
     5821 atttgtgcaa ggttgtgacg ttgaccgact tcaaagtcgt tagggtnnnn nnnnnncgtt
     5881 aggatcgtgt gcaggagtga ttttaacaca acctgtacca aattctttat caacatattc
     5941 gtccgcaata acaggaattt cacgtccgac aagcggaaga acaagcattt tacctactaa
     6001 atccttatat ctttcatcct caggattaac tgcaaccgct gtatcgccaa acaatgtttc
     6061 cggacgtgtt gttgcgatta taacgtaatc gtcactatcc tttattggat acttgatgtg
     6121 ccagaagtga cctgcctgct cggcgtgttc aacttccgca tcagaaagag ccgttataca
     6181 atgcggacac cagtttataa ttcttgaacc ttgataaata agtcccttat tatatagatt
     6241 tacgaatact tctttaaccg ccttactgca gccctcgtcc atagtaaaac gttctctgtc
     6301 ccagtcacat gaagaaccga ttttcttcaa ctgatttata attcttccgc cgtactgctt
     6361 tttccaatcc catactcttt caaggaactt ttcacgtccc aaatcgtaac gggtaagtcc
     6421 ttcgtttacg cgaagatttt cttcaacctt aatttgtgtt gctataccgg cgtggtctgt
     6481 acccggaatc cataacgcac tgtaaccttg cattctcttg tatctgataa gaatatcctg
     6541 aagtgtttcg tcaagtgcgt gtcccatatg aagttgacct gttacatttg gaggtgggat
     6601 aacgattgta aaaggtttct tgtttttatc cacttctgcg tggaaatatc ccttttctac
     6661 ccactcatta tacagcttat cctcaaattc agccggatta taattttttg ctatattctt
     6721 tgcatcttcc atttatacta actcctttta attttatctt cctaatataa ataccgccat
     6781 aaaggcaatt accgctaaag caagtgcaat gtatttgact cgcaggacca acccttgggt
     6841 cggctctttt ttgaaaactt tttcaagtat cagttcagct ttgaaagtaa gaactatcgc
     6901 tataagaccg ataataaaag ccgttatttt aataatcaaa aatactcaca ccctttcaat
     6961 ttatactcag aagaaaaaat actccattta attattatcg cattttaatg tatttttgtc
     7021 aaggtcaaac ttgtataata taagaaaata tgtagcataa taaattaata agcataaaag
     7081 cgg
//
LOCUS       DAWACS010000023         7031 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1210_length_7031_cov_2.256307, whole genome shotgun sequence.
ACCESSION   DAWACS010000023 DAWACS010000000
VERSION     DAWACS010000023.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7031)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7031)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7031
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1210_length_7031_cov_2.256307"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2218..2227
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4541..4550
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1865 a         1641 c         1216 g         2289 t
ORIGIN      
        1 gctgtgtacc catagttttt gaaaaaccgt tttgaggagt ggtcaaatca aaaagctttg
       61 tgtcatccac ataaacggta taatcaaaat acctcgacat aaccattaaa tactgcgaat
      121 catcaaacac attcactttg tcaatcttct tcgtcaatac aatcggttct cccgtatatt
      181 taaacccaca aggtaaaatc acttttgaat tatctttttc aacggtccac gtcttcaaag
      241 ttgcaaattc atcttccgaa agtattttgc atttgtacat atctcctttt agatacacaa
      301 acaaacctat aaaacaaata ataaacacaa gaatcagaaa catatattta ctgttgaatt
      361 ttcttagctt attcatagtt ttcattccat gctatcatat tagataaaac cgtttcggtt
      421 ttgtctactt taaacactca aaaattacat cgtaagatac gatttttgga gctgttggca
      481 ttatgtcgac agtctctcta tatatgtatt aattttattt taccattttc tatatggaat
      541 ttcaagagaa aaactgtgta ttttaaaaaa cgcattaaaa aaacagacaa aatcgcaaga
      601 attttgtctg ttttcgcgta ttacttattt catcaggaat ttatccactg cgtcagccgc
      661 ctgtctgccc tctctgattg cccatacaac aagactctga cctctgcgac agtcacctgc
      721 cgcaaacacg cctctaagac ttgtcttgtg atcctcttcc ttggcaagaa tgttgcttct
      781 tccgtcaaca tcaagagtaa gctgttttaa aagttcctgt tcaggtccta aaaatcccat
      841 tgcaataaga acaagttcgc aaggtcttac ctgttcagta cccttaacag gaacaggagc
      901 acctgttgac caatcaacct caacggtatg aacagccttt acattgccca tatcgtcacc
      961 ctcgattttt gtaaccgttg taagatactc tcttgggtct ttgccgtaaa gctcgatagc
     1021 ctcctcctga ccgtaatcgg tctttttgat tctcggccac tccggccacg gattattcgg
     1081 cattctttcc tctgaaagtt caggcataat ttcaagctgt gtaacgcttt tgcagccatg
     1141 tctgattgat gtacccacgc agtctgtacc cgtatcaccg ccgccgacaa caattacgtt
     1201 tttgccttcg gcacttatga acattctgtc ttcaagattt gagtcaagca aactctttgt
     1261 attagccttt aggaaatcaa ccgcataatg tacacccttt aagtccgcac cttcgcaagt
     1321 aagcattctc ggttttgtcg aacctgtgca aagtacaacc gcgtcaaatt catttacaag
     1381 tttaacagcc ggataatttt taccgatttc ggtgtcaagt ttaaatacaa cgccctcttt
     1441 ttccataagt tcgactctgc ggtttacgac tttcttgtca agtttcatat tcggaatacc
     1501 gtacatcaaa agtccgcccg ctctgtcggc tctttcaaat acagtaactc tgtgacccat
     1561 ttgattaagg tggtctgcgc aagccaatcc cgacggaccg ctgccgacta ccgcaacacg
     1621 tttttcagtt gacttttcag gtaatctcgg ctttacttta ttctccgcaa acattgtatc
     1681 tataatatgg cactctatat ttctaatcgt aacggcaggg tcgtgcatac cgagtgtaca
     1741 agaaccctcg caaagtgccg gacatactct gcctgtaaat tcagggaagt tgtttgtaag
     1801 cgaaagacgt ctgtatgcct cgtccatatc acctctgtat acaaggtcgt tccattccgg
     1861 tataaggtta tttaaaggac agccgattga cgttcttccg atttgcttac ctgtgtgaca
     1921 aaacggtaca ccgcagtcca tacatcttgc accttgaagc tgcagctgag caaggtcaaa
     1981 atgtgtatgg aattcgtccc aatctttcat tctttccttt ggaggtctgt ccttaggaag
     2041 ttgtctttga tattctaaaa atcctgttgg ttttcccatg attttaaatc ctttctgtaa
     2101 gagttaccgt tcggactgta taagcccgaa cgatatatca aacttattac gctatttcta
     2161 cttttttgcc cgttacattt tcaaatgcaa taagcattgc ctcatctctg tctgttcnnn
     2221 nnnnnnnaac agttataact ttcttatagt cgttcggaat taccttaaca aaattcttta
     2281 cttcttccga ccagttatta agcatatttt ccgcaacgtc actgcccgta tatttaacgt
     2341 gattttcaag catttccttt agctttgcaa tatccttctc tgacaatgtt tcaagtccca
     2401 caagttcctt gttacagttt ttgtcaagtg aaccgtcctt gttatataca tatgcaatac
     2461 cgccggacat acctgccgca aagtttctgc ctgtcttacc gattacggca acacaaccgc
     2521 ctgtcatata ttcacagcca tggtcgccga taccctcgac aaccgcagtc atacccgaat
     2581 ttcttacgca gaaacgttcg cctgccgtac ctctgatata tgcctcacct ttaatagcac
     2641 cgtagaatgc aacattaccg ataataacgt tttcactcgg aacaaattta gcctctttac
     2701 gcggtacaac aattatctta ccgccggaaa gacctttacc gatatagtcg ttactgtcac
     2761 cctcaagttt cattgtaacg cccggtgcca agaatgcacc gaaactctga cctgcacttc
     2821 cgacaaagtt caatttaatg ctgtcttcag gaagtccttc ctctccgtgt gctcttgcga
     2881 tttcagatga aagaattgta cctgttacac gattgatatt tgtgatttca agttttgcct
     2941 caatcttctt accgtcttta atcgccggtt cgcaaagttt caataatgtg ttcatatcaa
     3001 gagttttgta aagctcgtga tcctgcattt cattatggta tctctcataa tcgtttgaac
     3061 agattttcgg ttgatacaat agtgatgaca aatctacttc ttttgctttc cagttacccg
     3121 aaacagtctt ttggctgagt ctttcaacgt gaccaatcat ttcgtcaaca gtcttaacac
     3181 cgattttagc catccactct ctcaaatcct gtgcgataaa acgcataaag ttctcaacaa
     3241 attcaggctt accgcagaaa cgctttctca atttaggatt ttgtgtggca acaccgacag
     3301 gacaagtatc aaggttacat actctcatca ttacacaacc gattgagatt agcggacctg
     3361 tcgcaaaacc aaattcctct gctcccaaaa gtgccgctac cgcaacgtca cgacctgtca
     3421 aaagtttacc gtctgtttcg attacaactc tgtttcttag attgttcatc aaaagtgcct
     3481 gatgtgtttc ggcaacgcca agttcccaag gcaaacctgc atgacgcaca cttgtctttg
     3541 gtgcggcacc tgtaccgccg tcatagcccg ataccaaaat aacatctgct ctgccctttg
     3601 caacaccgac tgcaatagta ccgacacctg cttcagaaac aagtttaact gttactcttg
     3661 cgtcacggtt tgcattttta aggtcatgaa ttagctgtgc caaatcttcg attgaatata
     3721 tatcgtgatg cggaggtggc gaaatcaaac cgacacccgg agttgaatgt cttgcctttg
     3781 caatccaagg atatacttta ccgcccggaa gatgtccgcc ctcacccggt ttagcacctt
     3841 gtgccatttt aatctgaatt tcttttgcat tctgcaagta atgaatatga acaccgaaac
     3901 gtcccgatgc aacttgctta atagcactgc tgcggctgtc accgttttca tcaggaatat
     3961 atctttcagg gttttcaccg ccctcaccgc tgtttgactt accgccaagt ctgttcattg
     4021 cgattgcaag acattcgtgt gcttcctgtg aaattgaacc gtatgacata gcaccggtct
     4081 tgaaacgctt aacaatgctt tccacgcttt caacctgatc tattgcgatt ggcttatcaa
     4141 tcgtcttaat atcaagcata ccgcgaattg tgcattgatg ttgctgatgt tcgtccattt
     4201 cctttgcata ttctttataa agcttgtagt tacctgtacg acaagccatt tgtaatttgt
     4261 agattgactc agggttgaac atatggtatt cgccctttgc tctccactta tagtcaccgc
     4321 ctgttttaag tgccttttta ccgtttactt tatcaaaagc ctctgcgtgt cttaaaagca
     4381 cttccttttg gatatgctca atacccatac cgcctattct tgttgttgta cctgtaaagt
     4441 atttgtttac aacgctttcc gatataccaa gtgcttcaaa tatctgtgcg ccttgatatg
     4501 actgaattgt tgagataccc attttagaca taatcttaac nnnnnnnnnn ttcaagaagt
     4561 ttttcgtccg aaagctcctt aatagcctca tatgccatat acggattgat accgttcacg
     4621 ccgtaaccta caagacatgc gaaatggtgt acttctctcg gctcacccga ttcaagaacg
     4681 atagatactt tcattcttgt accttttctg ataaggtgat gatgaagtcc ggatgcaaca
     4741 agaagtgcag gaataggtgc tttatcctta tctactccct tatccgaaag tatgattatg
     4801 ttgtatccgt tttcgattgc tatatcagcc gcctcgaata ttgtatccat ggcggtttcc
     4861 atatcgtttt cctgttttac gttaaacaat gacggaattg ttattgactt aaatccttca
     4921 aggtcaatgt ttcttaattt tgcaagttct tcgtttgtta aaatagggct taatgctctg
     4981 accttacgac agttcaattc agatgatgtc aaaaggtttt gttcacaacc gaaaagtgtg
     5041 tatgttgaag taacaatctg ctcacgaatt gcgtcaatag gagggtttgt tacctgtgca
     5101 aacagttgtt tgaagtagtt atacaaaagt tgcggttttt ccgacaatac cgcaagcgga
     5161 atatccgcac ccattgcacc gattggatcg tcagcttttt caacgattga tttaatcgtt
     5221 gtgttgacat cctcccaggt atagccgaat gccttttgac gtgtaagcaa tgaaagtttg
     5281 tccacactgt tttcacgatt tacaaacata ttttcaagaa cgataaagtt cttcaaaagc
     5341 aaatgttcgt tgacattgcc ttttgcgttg tcctttaatt tatgcaccaa atcgtgccat
     5401 gtgtctccct ttttatctgt attaagagga agcttgtcaa gctcaacaag ggttttcttt
     5461 acccactctg catacggctt gtgaagtgcc tcttcctttt tgatttcttc gtctgaaata
     5521 atcttgccct tttctgtatc gataaggagc attttacccg gatgcagacg ttctttcttt
     5581 ataatagtag actcgtcaac atctgttaca ccgacttcag atgaaagaat aatcatatcg
     5641 tcagaggtca aatagtaacg agcaggtctt aagccgtttc tgtcaagcgt tgcaccgaca
     5701 taacgtccgt ctgtaaatgc aacagccgcc gggccgtccc aaggctctgt gatacatgag
     5761 tgatactcat aaaatgcttt cattgcaggg tccatatccg cattattttc ccaaggttca
     5821 ggaattgtca tcattacggc tctcggaagt gagaatcccg aaagatgtag gaactgcaaa
     5881 tagttatcaa gcattgcgga gtcagaaccg tcctcattga taacaggtaa aatcttatcc
     5941 atatcaccct caaattcagg tgttgcgaac attctttctc ttgccttaac ccagtttaca
     6001 ttaccgcgga tagtgttgat ttcgccgtta tggataatgt atctgttcgg atgtgctctt
     6061 tcccatgacg ggaatgtatt ggttgagaaa cgtgagtgca caagtgcgat tgccgtttcc
     6121 atatccacat ctttaaggtc aaggtaaaat tcattaactt gatcaggtgt aagcatacct
     6181 ttatatacaa ccgttcttgc agatagtgat gcgaaataga aatagttgtc aagaccgctc
     6241 tttctgattt ccttttctgc aagcttgccg ataatataaa gctttctttc aaatgaatct
     6301 gcatcaaggc tttcgtcacc tttgccgata aacacctgca cgatatgcgg cattgcctct
     6361 ctggcactgt taccgataca tacgtcataa acaggcactt ctcttatacc gagaagtttc
     6421 tgcttttcac cgtcaataat ctttgtaagt ctttcaagac tttcttctct tgtatcctta
     6481 tccggcgata aaaacagcat accaacgccg taatcaccct ttttaggtaa cttaattcct
     6541 tcatttttac aaaccttttt catgaaggca tgaggcattt gaattaaaat accggcaccg
     6601 tcacctgtat caggctcact gccgacaccg cctctgtgtg caaggttttt caatatctga
     6661 attgcctgat taataatgcc gtgagaagtt tttcctttga tatttgcaat aaaaccgatt
     6721 ccgcaggcat catgctcaaa ttgcgggtca taaaggccct gtttttcggg taatccatta
     6781 tactccattt ttttcaatcc tttcgctgta ttccgccgaa caaaaaagac gtttcaatca
     6841 gaacatacca atccataaat tgatatgtac cgatcgaaac gcctttgttt cgtcaaaaaa
     6901 ttattttgtt atcctgtgta aacttattat agcacattat ttttattttg tctactaaat
     6961 attttacaaa attgctttga ttttttccat tgcttttatg gtattttcct tagttgataa
     7021 tgctgtaaat c
//
LOCUS       DAWACS010000024         6975 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1228_length_6975_cov_1.949422, whole genome shotgun sequence.
ACCESSION   DAWACS010000024 DAWACS010000000
VERSION     DAWACS010000024.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6975)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6975)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6975
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1228_length_6975_cov_1.949422"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    422..431
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1492..1501
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2934..2943
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3400..3409
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4686..4700
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2398 a         1026 c         1539 g         1957 t
ORIGIN      
        1 tatttgtaaa gagttttatt ttaatatata ataatagaga aaataaaatt aaggagaggc
       61 actaatgata gtattttttg atttggacgg cacacttata tctgatgatg gggcatatat
      121 aatcccggaa agcgctataa acgcaattca taaagcacag gcaaacggac atcttatgta
      181 tgttgatact ggtaggacta ttatgaatat tgagcagcgc ttgttagata taggttttga
      241 tggttatata tgtggatgtg gaatgtatat agagtgtggc aaagaagtaa tatttcagca
      301 taaattaccg caaaaactat gtgatagtgt agcagaacta atatatgagt gcgatatgac
      361 accaatgtat gaacattcaa aatcattttt taccgataaa cgctgccgct catttgaagg
      421 cnnnnnnnnn ntaaaagacg ttttgaaaat cagggtaaga acttgtcacc cgacgtaagt
      481 gatgataatt ttgcttttga taagctgctt gcgtggtatg atgagaaaag gaacatgaat
      541 agatcgtgta ctcaggtatc atcatataca tttatcagat gcctatgcca taggcgatag
      601 tatgaatgat attcctatgc ttgaggcagt gccaaatagt attgcagtgg gtgaaagtcc
      661 agagaaacta aaacaactat cttcatttgt taccaagaat ttattagatg atggaattga
      721 gtatgcattg aggtatttca atataatatg atatatagtt cttttcaata agaacatagc
      781 atagaaaact attacagagg acatcgaagt agtttacagt ttgcttttcg caaattgcaa
      841 gcgttggtaa aataatagga attaaataaa taaaaaaata tatagttgat ctcagaatat
      901 acgggtacat gtgaacaccc cataataaga atatctctct ctcttttttt cttaaatata
      961 accgtgtatt ctgtgatgag tgatatattc aatatagtaa gaagtatatt caatatgcag
     1021 acacataact aatttcaata tttaatatat tgcggataaa aaagatttta ttataagcat
     1081 ataaagcgca ttgcgaaatg gctaaaaacc gttttgtaat gtgccttgtt ttttataacc
     1141 attttttggt atatgacaaa tttaaaaata tattgtataa tggttgtgga ggaaattatt
     1201 gatgttacaa aaaattaagg aatttgagag ctaccttatt ttggaggaaa agtcaaaagc
     1261 gacagtgatg aagtacactc atgatgtaac cgcatttgtt cagtggcttg acggacaaca
     1321 aatcagcaaa gcggttgttc aggaatataa gcaattgctg gagagtgaat atgtgcctaa
     1381 aagtgtaaat tcagtattat catcgctaaa ttcatttttt ggatggataa atcatccgga
     1441 atacagggaa aaaagtctta aaattcagaa aaacacattt attgaaccaa annnnnnnnn
     1501 ncttaaaaca gcttacgaac agggaaaaac acgtttgtat cttgtaatgc agacaatttg
     1561 tgctaccgga atccgtgtgt ccgaattggc atatataacg gtagatgcag tgaaaaacgg
     1621 tgttgcaagc attaaatgca agggaaaaaa tcgtgttatt ttcattccga gcaagctgtg
     1681 taagatgtta cagcggtaca ttagggaaaa gaatattaaa aatggagctg tgtttgttac
     1741 cagaaaagga aatccactta atcggagtaa tatctgggca gagatgaagc gtctttgcat
     1801 taaagcagga gtgcttgcaa gcaaggtgtt tccgcataat ctaagacact tgtttggtaa
     1861 aacatattat gctgtttatc aggatatagt ccgtctttct gatattcttg gacattcgag
     1921 tataaatacc gcgcgtattt atacagcgga aagcggagaa gtacaccgca agagaattca
     1981 aaatctcggt cttatacggg actattttct acacgataca acataataat tgattatgtt
     2041 gtgattatat cattcgcaaa agtaattgtc aataatttcc actacatttt agacaagaat
     2101 cgttcgtgaa tataatctat gaaatgttga ttttagacag ttttttataa tattttcagc
     2161 gcaggtgaca tggcaaaagc tgtgccgtct tattgttgtg ctctcaaata aattgtggaa
     2221 taacatactg ttgattatat aaatggtcag tggaagaacg taaaaagaaa gtcccggaag
     2281 acaatgctgg gacagaataa gagcaagcgg atacattcct atatgaatga gccagacgac
     2341 agatacgcca taatctataa tttgcttcta cctctttgta taatttcttt cattgaagta
     2401 tatagattgc gataaattat caatgccgaa aaacagaatt tgcaagctgt gggcgataat
     2461 cttgtatagg gacaatgata cttctgtcca gccgtagacc gcgctataaa aggcgtgtat
     2521 gcaagcatcg gggacagtcc gggcagagcg ccgggaaggt gaaattccta tggagcagtc
     2581 ttgcaagctg ctattcgttt tgcctatata gggggtgata tatttttagg aggtgcatat
     2641 tttggataaa gcttcggtta aaaaaattcc gatatcagaa aagtatttac tgacaattaa
     2701 agaggcgtcg gagtatttta atatcggaga gaataaaatg tacaggatag ttaatgattt
     2761 tttggaatcc gattataaat ttattgttca aaatggtagt agaacaatga taaacagaaa
     2821 aaaatttgaa tcctttttag atgaaacgac atcaatttag gaaatataaa aaaatagtag
     2881 aaatttagtc atagatgtga tatactaata ttacactatg gcttcttttt gttnnnnnnn
     2941 nnnggataat ggtttatacc aatttgacta tgttgatata tttggtaagg caaaatgtgt
     3001 ttacagttgg aaacttgaag aaactgatcc gcttcctaaa ggaaagagaa aatgtatttc
     3061 attaagagaa aaagaaaaag aaatattgaa ggatattgat gatggactta taccatatgg
     3121 tggtaatctt acggtgtatg aatatgttat tcaaagaaga ggagtgcgag aaaatacaag
     3181 aacaggttac aatactgtat tgagacgctt agagaaagaa ccttttggta tgcgtaggat
     3241 tgatgaagtg aaaatttcag atgcaaagct ttggcttata aagcttcagg aaaaggatgg
     3301 cttaagttac agtaccattc atagtataag aggtgtttta cgaccggcgt ttaaaatggc
     3361 tctggatgat ggacttatac gaaaaaatcc gtttgatttn nnnnnnnnng cattgacaca
     3421 tgatcaaaaa agaaaatttt tggcttttat caaaaatgac aagcattttt gtaaatatta
     3481 tgatgcaata tatattctgt tttatacggg tatgcgtatt tcagaattta ccggattgac
     3541 tataaatgat attgatttaa aagcaaagaa aatcaatatt gaacgccagc ttatacgccc
     3601 aagtaatatg aggtatacta tatgaaaaac ccaaaacgaa ggcaggaaaa aggattatac
     3661 cgataacaga taatgtatgt caatgttttg aaaggattct taataacaga acgtccttaa
     3721 agattgagcc tgtcgtagat ggttattcgg gatttctcta tttagataaa aacaatatgc
     3781 ctactgttgc aatgcattgg gaaaagtatt ttcagcatat ccgcgaaaaa tataatagca
     3841 tatataaaga agaattgccg ctaataacac ctcatgtttg cagtcatatg gcaagtgcag
     3901 gaatcaatcc aaaacattta cagtatttga tggggcattc agatttaagc gttactatga
     3961 atacatacac tcatgcagag tatgaaaatg ttgagaagga gctaaaacag ctacaatgcg
     4021 ccgtcctgta aattcgtcaa aatcgtctta ttttacagag gatttacaga aaatgaccat
     4081 caaattaagt ggcaaatcat aagaaattaa cagataatgt aaagtgtcga aaaatgtagg
     4141 catagagcca aaaataaggg tttaaaggag tttaaaagag tttatgaata tactgtttgt
     4201 atgtacaggc aacacgtgca gaagtgcgat ggcggcggcg ataatggaca aaatcgctgt
     4261 tgaaaacgac cttgatgtat ttatcgaatc agccggaata tttgccgagg acggtcaagg
     4321 tgcaagtgaa aatgcgatta aagcacttat gaaatacggc atagacttat caggccatag
     4381 aacacagcct gtaacggagg accttataaa gcaatgtgat ttaatcctaa caatgacaga
     4441 gggacacaag caaatacttg agcctttggc aaaaggcaag gtatacacat tacttgaata
     4501 tgcaggttca agcggtgaca tatccgatcc ttacggcggt gaccttgaag aatatgaaga
     4561 agttgcacaa gagatttatg acgcgcttgt agacattgcg gaaaaaatcg cagatatgca
     4621 aagtaaaaag tgatatgaaa aatattacga taacacgaat gacagatgat gacgttgcgg
     4681 aaattnnnnn nnnnnnnnnn gcttgaaaaa aagtgctttg cagttccgtg gtcggaaaaa
     4741 tcttttcacg acgaaatgca aaacaaactt gcggtgtatt ttgtcgcaaa ggataatggt
     4801 aagtgtatag gctatgcagg gttttggaac gtgtcgggtg agggtgacat aacaaatgtc
     4861 gcggtacttc ccgaatacag aaaaaacgga ataggcagta tgcttatcag tgaaatgata
     4921 aaaacagcca ttacattaaa gctggaactt ttaactcttg aagtccgcaa aagtaacata
     4981 gcggcccaag ggctttacaa gaaatacggt tttgatataa taggtgagag gaaaagatat
     5041 tacagtgata acggtgagga cgcttggata atgacgaaaa gctttgaacc gtgtgaggag
     5101 tgataacgtg cttatacttg gaattgaaag ttcatgtgac gaaactgcgg ctgctgtcgt
     5161 aaaagacggc agagaggttt tgtctaatgt aataaatacg cagattgcac tgcacaaaaa
     5221 attcggaggt gttgtgccgg aggttgcgtc aagacggcat attgaaacga ttgacgcggt
     5281 tatagacgag gcattggagg aggcaaacgt aacatttgac gatattgacg cgattgcggt
     5341 tacatacggt ccgggtctcg tcggtgcgtt gcttgtcgga gaatcaacgg caaaagcact
     5401 tgcgtttgca cttaacaaac cgcttgtgcc ggtacatcac ataaagggac acataatggc
     5461 taattttgtg gcacacaaag atttagaacc gccgtttgta tgtcttgttg cgtcaggcgg
     5521 acacagtcat attgtgagcg ttgaggatta cacgcatctt gaaataatgg gcagaacacg
     5581 tgacgatgcg gcaggtgagg cgtttgacaa aatagctcgt gtgcttggtt tgggttatcc
     5641 gggcggcccg cttattgata aacttgccaa agagggtaat ccgaaagcgg ttgatttccc
     5701 acgcgtgaga atggagaaaa attcgcttga tttctcattc agcggtgtta aaacagcggt
     5761 cataaattat cttcacaaac ttgaacaaaa cggtgaagaa tataataaag ccgacattgc
     5821 cgcaagcttt caggatgcgg ttacggacgt tttgtgtgaa cacacgatag aggcggcaga
     5881 acagaaaaac agcaagatta tcgcacttgc aggcggtgtt gcgtcaaaca gtgcgttgcg
     5941 tgaaaaaatg acaaaacttg caggcgaaaa gggcataaaa gttgtatatc cggagcctat
     6001 actttgtacg gataatgcag taatgatagc ctgtgcggga tattacggtt atcttgaaaa
     6061 gaattttgcg gatatgacgc ttaatgcgat accgtcactt tcactataag gggacactaa
     6121 aaacagggac actaaaagga atattgaata tggaaaaatt atttgttgat ttaggaaaaa
     6181 aatcatacga tatacttttt tcggatagct ttaacggctt tgcaaaagca atgtcggata
     6241 taaatgcacc gaaaaaatta cttgttgtaa cggacagtaa cgtagaaaaa ctatatgccg
     6301 acgaagtaaa tgatttactt ataaaaaacg gctatgattc aaaaatatac gcatttacgg
     6361 caggtgaaga aaacaagggg atggatacaa tactcggcat atgcggtgcg tgtatcgaac
     6421 acgaaatgga cagaaaaagt atgattgttg cacttggcgg cggcgtggta ggagatatgg
     6481 caggatttgc cgcggcaata tttatgcgcg gtattgattt tgtacaagtg cctacaacac
     6541 tactttcaca gtcggacagc tctgtcggcg gaaaaacagg tattgacttt atggaaagca
     6601 agaatatttt aggtgctttt catcagccga aacttgtgta cataaatgtg gcaactttga
     6661 aatcacttcc gaacgaacaa tttgtttcgg gtatgggaga ggttatcaag cacggaatta
     6721 taagagacag cgagttcttt gattatatcg agaaaaacag cgacaagata aaaacacttg
     6781 acaatgaaac acttataaag atggataaga taaattgttc gattaaggct gacgttgttg
     6841 agcaggacga aaaagaaaac ggacttcgtg caattctcaa tttcggtcat acaatcggtc
     6901 acgcagtcga atcggcatac gattttaaga tgactcacgg tgagtgtgtc ggaatcggta
     6961 tggtcggtgc atcgt
//
LOCUS       DAWACS010000025         6967 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1230_length_6967_cov_2.498264, whole genome shotgun sequence.
ACCESSION   DAWACS010000025 DAWACS010000000
VERSION     DAWACS010000025.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6967)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6967)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6967
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1230_length_6967_cov_2.498264"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2001 a         1614 c         1174 g         2178 t
ORIGIN      
        1 actttatatt ccgcaatcaa ttatatacct atttccgata aatttcaaca attagtttac
       61 acctttataa gcgtctctca taatctgttc aatttcctta acaagcggca acttcggatt
      121 tgcagttgta cactgatcct caaaagcctt gtttgcaatg tcaggaacat ttctcatata
      181 agtttcttca tcaattccgc attcagagaa tgacaacggc tcattaactt ctttcataag
      241 gtctataacc gcatttataa ggctctgtac accctcttct gttgtttttg caggaagtcc
      301 caagaatgct gcgatttcag catacttttc atctgcaatg aacttcttat acttagggaa
      361 tgacacaaac tttgaaggtg tcgacgcatt atacttaatt acgtgcggca acaatactgc
      421 atttgctctg ccgtgaggga cgtgatactc accgccgatt ttatgtgcga ttgagtgatt
      481 tagtcccaag aatgcgtttg taaatgccat acctgccata caacttgcat tgtgcatctt
      541 ttctcttgcc tctgcgtcct cagcaccgtt cttgtatgct ctcggaagat acttgaatac
      601 aagctgaata gctttcattg caagaccgtc tgtgtaatcc gacgccatta cagatacata
      661 cgcctcaagt gcgtgtgtaa gcacgtcaag acctgtgtcg gcagttgctg atttcggaag
      721 tgacattaca aactgcgggt cgataatcgc aatattcggt gttagttcgt agtctgcaag
      781 aggatacttc atattattct tcttatccga aataaccgca aaacttgtta cttcactacc
      841 tgtaccggaa gttgtcggaa tagctacaag ttgtgcctta ttacccatct tagggaactt
      901 aaacgcacgt tttcttatat caaggaaacg aagtctcagt gaattaaagt ctacatcagg
      961 gtgttcatag aacagccaca taccctttgc ggcgtccatt gcactgcctc cgccaagtgc
     1021 gataattacg tcaggcttaa attcgtccat catctgtgca ccgcgtttaa ctgtttcgat
     1081 tgacgggtca ggctctacat ctgagaaaat ttcacaatga acgtgctccg ttctctttct
     1141 taggtaatac aaaatcttat ctacataacc aagagaaacc atacccgggt ctgttacgat
     1201 aaatgcacga gtgatattcg gcattttttc aaggtactga attgaattat gctcaaaata
     1261 aatcttatcc ggaactttaa accactgcat atttactctc ctcttagcca ctctcttctg
     1321 atttataagg ttcaccgttg taacgttacc gctgacactg ttaccaccgt agctaccgca
     1381 accaagtgta agtgacggca tatttgtatt ataaatgtca ccgattgcac cgtgtgatga
     1441 cggtgaattt acgataagac ggcttgcttt cattgttgca gcatactttt gaattacatc
     1501 ttcatcattt gcgtgaataa cagccgagtg tcccataccg tggaacataa ccatttcttc
     1561 tgctctgtcg attccttctt ctgttgaatc aacgataaag tatgcaagta ccggactcaa
     1621 cttttctctt gaaagcggat attcagggcc tacgccgtca agttttgcaa gaagaatttt
     1681 cgtaccctca ggaactttca ctcccgcttg ctccgcaatc catgttgcag gcttacctac
     1741 aattacaggg tttacagcac ccttttcact gtcgatgcag taatcgctaa gctttttaat
     1801 ttcgtcaggc tttgcaaagt agcagccgta tttcttcata agtctttcaa atttcggtgc
     1861 gattgcctta tctacgattg cggcttgttc agacgcacaa atcataccgt tgtcgaatgt
     1921 ttttgaaagg ataaggtcgt taaccgcttg ttcaaggtca gcactcttat gaatgtagca
     1981 aggtacgtta cccggaccga cgccaagtgc aggcttacct gttgagtatg ccgccttaac
     2041 cattccgctg ccgcctgtcg caagaactgt tgcaacaccc ggatgattca tcagtgcgtt
     2101 tgttgcctcg attgatgggt attcaatcca ctgtatacag ttttcaggtg cacctgccgc
     2161 aactgccgca tcaagaacta tcttggcagc cgccgcactg catttttgag cgcccgggtg
     2221 gaaaccgaaa ataatagggt ttcttgactt aacggaaatc aaagccttaa acattgttgt
     2281 tgatgtaggg tttgtaacag gtgtaacacc acatacaacg cctacaggct ctgctataat
     2341 ttcgtaatct tcaaggtcgt tttcagcaat aacaccgact gttttttcat tcttgattga
     2401 atgataaaca tattctgttg caaacatatt ctttgttatt ttgtcctcat acacgcctct
     2461 gcctgtttcc tcaacagcca tctttgcaag ctccatatgc ttgtcaagac ctgcaagagt
     2521 cattgccttt acgattgcgt caatctgttc ttgattaagt gccataaatt cgttcaaagc
     2581 tttttgtgct tttgcaacgt agccgtcaat catttcattt acgttaatct ctgccataat
     2641 aattccatag cctttctgcc tacgggttgt tagtttttct ttacatctta ttgtaggctg
     2701 ataagatgtt tttacagaag actctgtttt gacatagtat gaaatcttcc gaatttttat
     2761 tattgacttt gtttatttag attgataaat ttttatcgtt atttatttat caatctttac
     2821 aacactatta tacgagattg ttaaactttt gtcaatagtt tttttgatat ttttattctt
     2881 tgtaagtttt tcatatataa ttcattactt tcttgtttaa aacaaacaaa aaaagtattg
     2941 cattttcagc aatacttttt atatacattc ttttatatac tttccaatct gtttatcgac
     3001 tcgatatctc ctacaagcac gacaatatcg gtatctttaa atttgtattc gggttcaggt
     3061 gaaatattca ccttaccgcc gttattactc tttatcgcaa caatatttac gttatatttt
     3121 ttacgaacag acaattccat aatgctcttg cctatccatg tgttcggtac aacagcgtcc
     3181 acaatactgt attcgtccga caattcaatt atatcaaaca atctgtccga aacaagttct
     3241 attgcggctt taatgcctgc ctcctgttcg ggaattataa cattatccgc accgactttt
     3301 ttcagtacgg ttttgtgcat attatccttt gctttacaga caacctgctt aactcccata
     3361 tcttttagtg caagagttgt aagtacgctg tccgttatat cgtcaccgcc aatacacacg
     3421 attgcacaat ccgagttttt cactccaagt tgttttaaca agctttcgtc ttttgcgtcg
     3481 gtacaagccg ctcttgttac caaatttgca atagcgttta ttttatccat atttttgtct
     3541 atcgcaagca cttcataacc catattaaaa agttgcattg caacagatgt tccgaacctg
     3601 cccaatccta ttactgtaaa agttttcata acaaatccct agcctttctg ctatccgaca
     3661 atgaaactac attccggata ccttatatca ttagtttttc tgatgcgagt cataaacaca
     3721 acgcttatcg tcataatgcc cactctgccg aagaacatat acactataag caaaatcttt
     3781 gtaaatgtgg acgcgctccc cgatacaccg accgaaagac cgaccgtact gtatgccgac
     3841 gcagcttcgt atatcgaatt tatcaaatct tgattatcaa tataacttat aagcaccgaa
     3901 ccgcccacaa caagcataag ccacaagaca agcaatgcca ttgcgtgaag aatagtcgtg
     3961 tgtgcaactc ttctgccgtg aataacaaca tcagatttgc cacgcaatac ggaaatcaaa
     4021 gtcattatta caagtacagc cgttccgagc tttacgccgc ccgcagtcga gcctgaagcg
     4081 ccgcctatca tcataagcac cgttccccaa gcctttgaca agtcggttaa attattctgt
     4141 ataagtgcgt caaatccggc cgttcttgtt gtgaccgact gaaagaatga cgccaatatt
     4201 ttctgaccga cactttcagc accgattgta tttatgttgc cgtattcaag aatgaaatac
     4261 acaaatgtac ccacggcaat aaaaaatatc tgtgccaaaa ttattacttt tgaatacacg
     4321 ttaagtcgtt tccacgattt ttttgcgata atatcctccc acacaataaa tcccaatccg
     4381 cctatggcaa caagtatcgc aagcgtacaa agtacaagcg gatcgtgtct gtatgaaagc
     4441 atactttttc cgtcaccgat tatatcaaat cccgcattgc aaaatgccga tacggaatga
     4501 aatatactaa accacattcc tttaaaaaat ccgtatttcg gtacaaaacg tattgaaagc
     4561 actatcgcgc ccaacaattc tataattccc gttatataca aaacgtgctt tgcaagtttg
     4621 actattccct caaggctttc cgtacccatt gtctgagcta taagcattct gtttctaagt
     4681 ccgatttgtc ggtttgaaac tataaacaca atacaaagta tcgtcataaa tcccaaaccg
     4741 ccgagctgaa ttaaaaacag tatcaccgct tgtccgaaca agctgaaata cgcaccgaca
     4801 tttataagcg taagtcctgt tacgcaagtt gcggacgttg ttgtaaacag taccgacaaa
     4861 aacggtgtca caactctttc ttttgacgaa atcggcaaca ttaaaagtaa tgctccgaca
     4921 aggataatca acgcaaatcc aaacgaaata caccttgccg tatttattct tttgtgaaat
     4981 aaattttttc tgataatatt catatttatt tgcttcctcc aaaaaatact caaaaatcac
     5041 atcttccgat gtgattttcg gagctgtcgt caggctcttt aagttcgttt ccacgcatac
     5101 ggtgaaaaca atataagtat cggataaaat tcaagtctgc ctacaagcat acctattgac
     5161 atcgctattt ttgacaatgc cgagaaatcg gcaaaactgc ctgtcggacc taccaatccc
     5221 aatcccggac caatgttgtt catagtcgca attacggcag taaccgttgt tgtaaagtcc
     5281 ttattatcaa agctgataag aagaattgac acagccgcaa acaacatata tacaacgaaa
     5341 tatcccaaca caccgtttac aatattcttt tcaaccgttt tgccgtcaac cttaaccgaa
     5401 aatacacttc gcggacttac cgcttttttt attgagtgag tggctgtttt cataagtata
     5461 ataaatcttg taactttcat accgccgctt gttgagcctg cacacgcgcc gactaccatc
     5521 aataatacaa gtataatctg tgaaagcata ggccaacgca caaagtcggc agttgaatat
     5581 cccgtcgttg tcataattga cgacacttgg aagaatgaat atctgaacgc tctgccaaat
     5641 ccgtgataca taggcataat attaagtgca ataattaccg ttgcacctat catcattcct
     5701 atataccact taacttcgtc attactcttt atctgcgaga attttcttgc aattatcaag
     5761 aaatacacgt taaaattcaa tccgaacaac atcataaata taccgataac ataatcgata
     5821 tatgcactgt tataatacgc aacggaacta ttctttattc cgaaaccgcc tgtaccggct
     5881 gtgccgaacg tatggagcaa gctgtcaaat acaggcatac cgccgaacag caaaagcaca
     5941 aattcaatga ccgtaagcgc tatatatatt ccgtaaagta ttctaagcga aaactgccat
     6001 ctgcttgcaa gtttgcctac aacaggtccc ggcatttccg ctctcatcat atgagttgtt
     6061 cttgtatcct ttgacgaaaa aatcgccatt gcaaacgcga gtacacccat accgccgagc
     6121 caatgagtaa agcttcgcca aaaaagcaag ctcttgtcaa gtgcctcgac atttgtaagt
     6181 atagatgaac cggttgttgt aaaacctgac accgtttcaa aaaaactgtc cacaaaactc
     6241 ggtatctgac cgctgatata aaacggcaga caaccgatta ttgatattac aatccagcct
     6301 atcgcaacga taaacattcc gtcacgtccg taaaggcttt tatttttcgg tgcttttgca
     6361 gatacacaaa gtccgattaa tacggatatt actatcggta ttacatacgc caaagctatt
     6421 gactcaccgt aaagcaaaga caatcccaaa ggtgcaagca ttgttgccgc aacggctaca
     6481 agtattcgtc ctacggaata tgctatcatc ctataatgca tatcttttcc tttcgtaacc
     6541 gctatgcaag tatatcgccg agtgtggcaa tatgatgcgt tgtcataatc acaaccgtat
     6601 caccgacttc aaatttactt tcaccgtccg acagcataat cttgttattt ctgagaattg
     6661 ccgcaatcaa agcattacgt ttcatcttaa tctgtgaaag tgactttgaa gtaagctcgg
     6721 attctcttgt aactctgaat tccatagctt cagctttatt gtttactatt cggtaaagac
     6781 ttctcatttc cgtatccttt gccgatttca tcgctcttgt gtaaccgatt ataatatttg
     6841 cggttatgtt tttaggcgta acaatactgt acacgcctgt gttttccata agctccgcaa
     6901 acgaaagacg gtttaccttt gttactatct tatcgacaaa tcttgactgc gaatacattg
     6961 atataat
//
LOCUS       DAWACS010000026         6940 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1235_length_6940_cov_2.092665, whole genome shotgun sequence.
ACCESSION   DAWACS010000026 DAWACS010000000
VERSION     DAWACS010000026.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6940)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6940)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6940
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1235_length_6940_cov_2.092665"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1463..1472
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3187..3196
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1977 a         1232 c         1528 g         2183 t
ORIGIN      
        1 ttgagtaata atacggagaa acagcctcaa attcacccgc acaagtatca accattttgt
       61 aaacagggat aatacccaaa gattttctaa gattatatac ctcgtctgcg tcacaaccga
      121 taagttctga aatgccctta tcactaaagc ccattttctt atatttcttt agaatgtcat
      181 aattcaaatc ggctaattta tatttcttta attcttcttc tgcgtctacg atatttttaa
      241 ttttctgcaa gaagaatggg tccatacctg tgatttcggc aagcttttcc atattgtaat
      301 ttctgcggat aagctctgca aggtcgaaaa gtctttcgtc atcgggaacg ataacgcgtt
      361 gctttaattc aacggtggta cgtttctttg aagattttct ttcaagtgtg tattgtttta
      421 tttcaagtga acgaataccc tttagaagtg atgattcaag agtattgccg atagccataa
      481 tttcacctgt agccatcatc tttgtaccaa ggttacgctt tgcaccgaag aatttatcaa
      541 acggccattt tggaattttt gttacaacgt agtcgatagc aggttcaaag caagcgtatg
      601 ttttacctgt aacggcattc ttaatttcgt caaggttgta accaagtgca atcttagcgg
      661 caatcttagc gatagggtag cctgttgcct ttgatgcaag tgcggatgaa cggctcacac
      721 gagggttaat ttcgataacc gcatagttaa agctttccgg atcaagtgca aactgtacgt
      781 tacaaccgcc tttaatttcg attgaattga taatatcaat agccgcttta cgcaacattt
      841 gatattcttt atcagccaaa gtaagtgcag gagcgacaac gattgagtca cctgtgtgaa
      901 caccgacagg gtcgatgttt tccattgaac ataccgtgat acagttacct gcaccgtcac
      961 gcataacttc aaattcgatt tctttccaac ccttgattga cttttcaaga agaacctgac
     1021 ctactcttga aaggtgcaaa ccttgtgaaa ggattgtttc aagctgttcg ggatcttcag
     1081 caataccgcc gcctgtaccg ccaagtgtgt aagcgggacg aacgatgaca gggtagccga
     1141 ttttctttgc aaatgcaaga cccgattcaa catctgtaac gatttcagac ggagcaatcg
     1201 gctgatttgt gcgttccata agacgcttaa agctgtcacg gtcctcaccc tctttgatag
     1261 acggaattga agtaccgata accttcatat tatacttttc aaaaacgccc ttgtcgtaaa
     1321 gctcacaagc aaggttaaga cctgtctgac cgcccatacc ggcgataatt gagtccggac
     1381 gttctttttg aataatcttt tctacatatt ctgcggtaag cggctcaata taagtgtttg
     1441 tagccatacc tctgtcagtc atnnnnnnnn nnaaattcgg cagcctgtcc gattacgata
     1501 ggacctgagc ctataacaag aactttcttt atacttttat ctaaaggcat attagtttac
     1561 cccctttaaa aatctgtcaa ataaccaacc tgtatcaagc ggtcccggtg atgcttcagg
     1621 gtggaactga acgctttgga ttggtagagt tttatgtctg ataccctcgc aagtaccgtc
     1681 atttacgttt acaaatgttt cttcaacagt gtccggtaaa tctgcaacta tatagttatg
     1741 attttggctt gtaatgaaaa cattacctgt tgtaaggtct tttacagggt ggtttccgcc
     1801 gtggtgaccg aactttaatt ttttagtttc gcagccaagt gcaagaccta taatctgatg
     1861 tcccatacag ataccgcaga ttggatattt acctattaat tgcttaacag tatcaactgt
     1921 acccggagcg tcaagcggat cgccgggacc gtttgaaagg aatacaaggt ccggatttgc
     1981 ttcttcgata accgaagcgg gaatgttggc agggaatact gtaattttac agcctctctt
     2041 caataaatcg cggataatac cttgttttgc acccatatct atgaaagcaa cgtgcggtct
     2101 gcctgtttca tttaatgtat atacctgctc agcagttgtg tccataataa cgtgactgtt
     2161 gtcaagagag ttcataagta cattgacttc ttcatcactc ggctcgccct gcataatgac
     2221 acccttcatt gtacctgctt cacggataac tcttgtaagt gctcttgtat cgataccctc
     2281 aatacctaca acaccctgtt gacgaagaaa atcatcaagg ttcatttcat ttctgaagtt
     2341 agacggaaaa tcgcactttt ctcttactac aaatgcttta agattagtgt gctttgcctc
     2401 catatcctca agattaattc cgtagttacc gataagaggg aaagtcatag tgacaatctg
     2461 acctgcaaaa gacgggtcag taagagtttc ctgatagcct gtcataccgg ttgtgaaaac
     2521 gacttctccg acgatgtttt tctcagcgcc gaaaagcgtt cccgaaaaac gcataccgtt
     2581 ttccaaaata agctgtgctt tcataattgt tctccttcca tgttagtgaa ttaggctata
     2641 tagcccaaaa aaaaagacaa tgaataaata acattgtcaa ttggttttta aaaatgaaaa
     2701 tagaaataaa ggggaaacag aatacccttt gtctgctttt attgagttgt aattgcctac
     2761 gcagtacata ctgttttata tctcctttat gtcattttta ttattgtacc acgcaatgag
     2821 tggtttgtca acatatagta cggattttgt tggttggtgt caaggtttgt ttatattttc
     2881 taaataaata agtgcaaagt aacttttatt ttgtaatgtt agtcaaaatt aaaaaaagct
     2941 attgacattt taaatccata ggcgtataat gcaattaaag ttaaagagat tgaacacaag
     3001 ggcgtgttcg taagattgaa aaaaggtctt acgaatgcgc tttttcagtt caattaggaa
     3061 aacttaatat tatacattat aattaagcac aaaggcgtgt ttatcgaaag tatcagaaaa
     3121 cttcgataga cacgcttttt tgtttaaaag aaaggggagt ttagtcatgg cagaaatgac
     3181 aaaagtnnnn nnnnnntcga aattatcacc agacaaagca agcttgaaga tttaaaggcg
     3241 gctttgaatg aaatcggaat caacggtatg acagttacca atgttcttgg atatggtgta
     3301 cagaaaggtc agaaacatca gtacagaggt gttgagtata acgttgattt acttccgaaa
     3361 attaagattg aaatggttgt atgtacagta ccggttgaag atgttgtaaa tacagcgatt
     3421 tcggttttaa gaacaggcga aatcggtgac ggtaagattt tcatttctcc ggtatcacga
     3481 gttattaagg taagaaccgg tgaagaagac agagaggctt tattatagaa agtttcatta
     3541 aataggagga ttgattgatt atgagtacag aactattaac aacagcagta gactgttttt
     3601 ggttattctt tggcggtgta ttggtgttct ttatgcaaac aggtttcaca atggttgaag
     3661 caggctttac aagaagtaaa aacacaggta acattattat gaagaacata gttgacttta
     3721 tgttcggttc aattatttat tggtttatcg gttacggact tatgtacggc gaaggtaacg
     3781 ggtttatcgg tattccgttc ggagatatgt ttatgaacgg tatggcaggt gcagagaata
     3841 tggattatac ggcattgttc ttccaaacag tattctgtgc aacatctgca acaatcgttt
     3901 cgggtgcaat ggctgaaaga acaaacttca aagcatattg tatctacagt gcggctattt
     3961 cattgtttat ttacccaatc gcaggtcact gggtatgggg cggcggctgg ttgtcagagt
     4021 taggtttcca tgactttgca ggtagtgcaa tcgtacatat gcttggcggt acattatcat
     4081 ttgtaggtgc tgcaattctt ggtccgagaa tcggcaagta cacaaaagac ggaaaagtaa
     4141 acgttattcc gggtcacaac attcttatcg gtgcacttgg cgtacttatc ctttgggtag
     4201 gttggttcgg atttaaccct gcatcaacag gcggtctttc agacggtggc gtacaagtag
     4261 ccgctaaggt atttatcaca actaaccttg ccgcttgtgc agctgcaatc acaacaatgt
     4321 tctttacatg gattcgttac ggtaagcctg atgtttcaat gactctaaac ggtgttcttg
     4381 gcggtcttgt tgcaatcaca gctccttgtg accttgtaac tccgtttgcg tcaattatta
     4441 tcggtgttgt cggtgcaatt ttgatgtgtc ttgcaattcc gtttatcgat acaaagctaa
     4501 aggttgacga ccctgtcggt gcaatcagtg ttcacggtat atgcggtctt tggggtacaa
     4561 tcgcagtcgg tttgtttgct tcagatacaa gtgccggtgc agaattactt ggtctgttct
     4621 atggcggcgg tctaaaggct ttgggtgttc aagctttggg tgctgtttca ctaatcgctt
     4681 gggcagcaat cgttggttca attctgtttg ttgtattgaa gaaggcagga ttgcttcgtg
     4741 ctaagccaca agaagaaatt gaaggtcttg actcaacaga acatggtctt gcatcagcat
     4801 accctgactt tgttgtatcg tataacgaga aaagatcaat gtaatttaat aaggttaatc
     4861 tctttttcaa agattaatat atataccccc tcagcgagct aatttattag ctcgctttct
     4921 ctttttagaa aaatttcaaa aaaactgttg acaacaaaaa gttagtgttg tataataatg
     4981 ttacggtgat tgaacacaag gacgtgtttg taagagaata ttcttacaaa tgcgtctttt
     5041 ttgtttttac ggttcaatcg gaataaaaaa taattagtta aattaaacac aaaggcgtgt
     5101 ttgatggatt gattgcggtc aatttgtcag acgcgctttt tatgtttaag agaaagggga
     5161 gtatatagtc atggcagaaa tgacaaaaat cgaaattatc acaagacaga gcaaacttga
     5221 ggatttaaag acggctttaa acgaaatcgg aattaacgga atgaccgtat ccaatgtatt
     5281 gggttacggt gtgcaaaagg gacaaaagca ccgttacaga ggtgttgagt acagcgttga
     5341 cttacttccg aagataaaaa ttgaaatggt tgtatgtaca gtgcctgttg atgatgttgt
     5401 taatacggca atttcggttt tgagaacagg tgaaatcggt gacggtaaga tttttatttc
     5461 acctgtatcg agagttataa aagtaagaac cggcgaagag gacagagagg ctttattata
     5521 ggggggtata aaatatgtta aacagtggag atataggatt tatgataatt gcggcaacgt
     5581 atgtgttttt gatgacaccg ggattggcat ttttctatgg cggtcttgca caaagaaaaa
     5641 atgtgctcaa tacaatgatg atgtcagtag catttatggg actttcatcg gtattgtggg
     5701 tactttgcgg atattcgctt tcattcagcg gtgacttggg cggagttata ggaaacttaa
     5761 agtggtttgc gtttaacggt gtcggagcgg aggcaggtcc ttacagtgac aatatcccta
     5821 atatgggttt tgcgttgttt cagatgatgt ttgccattat aacaccgtcg cttattacag
     5881 gtgcggttgc gggaagaatg aaatttaaag caatattctt atttgtattc ttgtggcagt
     5941 ttgtggttta ttatccgatg gctcatatgg tttggggcgg cggatttctt gctcaaatcg
     6001 gctcaatcga ctttgccggc ggtaacgttg tacatataag ctccggtgtt tcgggtcttg
     6061 tgcttgcaat attaatcggt aagagaaaag gcattgagaa aagaagtgtc gtaccgcata
     6121 atattccgtt tgttgtactc ggcgcgtcac ttttgtggtt tggttggttc ggatttaatg
     6181 caggaagtgc acttgcggca gacggtcttg cacttcacgc atttacaaca actaacactt
     6241 ctgcggcttg tgcaatgctt tcgtggatga ttatagatat aatcaaaggc ggaaagccga
     6301 cagtagtcgg agcttgtaca ggcggtgtta tcggtttggt ggcaattaca ccgggtgcag
     6361 gtttcgtacc gatatgggcg tcgtttatta tcggtgcact tgtcagcccg atatgttact
     6421 ttgcattatc tgtattaaaa ccaaaattcg gatatgatga cgcacttgac gcattcggct
     6481 gtcacggtat cggcggtata tggggcggta tcgcaacagg cttgtttgca caaacttcga
     6541 ttaatccggt agctaaatgg gacggtcttg ttttcggtga ttatcacttg ttcgtggcac
     6601 aggtgcttag tatattggta acaatagccg ttgcggttgt cggaacattg atttgtgtgg
     6661 gtattgtaag attgtttaca aaacttcgtg ttgatgaaaa cagtgaaaat gtcggtcttg
     6721 acgacagtga acacggcgaa tgcgcatatt cggcaatatg ggagtaaagc atagaaagat
     6781 gacaaaaggc agacaaaatc attatggttt tgtctgctat ttttttattt aaaaatatct
     6841 cttgcaatgt attatctttt tcgataaaat agtagtaaaa tatgtaaacg aaagaagtat
     6901 attgatgaaa aaggaaaaag ttcttaaaaa atatttcgga
//
LOCUS       DAWACS010000027         6860 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1249_length_6860_cov_2.779868, whole genome shotgun sequence.
ACCESSION   DAWACS010000027 DAWACS010000000
VERSION     DAWACS010000027.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6860)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6860)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6860
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1249_length_6860_cov_2.779868"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4324..4333
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2199 a         1192 c         1579 g         1880 t
ORIGIN      
        1 cccttgtacg gttatggcag gcggtgttcc tgttccgatt gaaacaaaag aagaggacaa
       61 tttcagactt ttgccggaac agctgaaaga aaagattacc gacaagacaa aacttttaat
      121 tctgccgtat ccgaataatc cgacaggcgg aattatgtca aaggaagatt tggaggctat
      181 tgcagaggtg cttcgcggta caaatataat ggtactgtca gacgagattt atgctgaact
      241 tcgttatgac ggcgaaaagc atattccgtt ttcaaatttt gagggtatga aagaaagaac
      301 tgttgttgta agcggttttt caaagacatt tgctatgacc ggttggcgtc ttggttatgc
      361 gttaggtcct gaaagaatta taaagcttat gacaaaaatt catcagtacg gcataatgtc
      421 agcaccgaca accgcacaat tcgctgcgat tgaggcacta aagagctgtg acgatgacgt
      481 tgcggaaatg cgtcgtgagt ataattacag aagacgctac atagtggacg gtttcagagc
      541 aatggggctt agttgttttg aaccgctcgg agcattttat gtattccctt gcataaaatc
      601 tacgggtatg acaagtgaag aattttgtca aaatctgctt atggaagaaa aggtcgctgt
      661 cgtaccgggc aatgcgttcg gagagagcgg agagggattt atacgctgtt catatgcgta
      721 ttcgattgag aacattcaag aagcacttaa aagaatagag aagtttgtta aaagacacag
      781 taaataggag gaaaaaatgg atacaaagta tatattcgta acaggcggag ttgtttcagg
      841 actgggtaag ggcattacag ccgcatcatt gggaagattg ttaaaggcaa gaggatataa
      901 ggttacaatg cagaagtttg acccttatat caatatggac ccgggtacaa tgagtccgta
      961 tcagcacggt gaagttttcg ttacagatga cggtgcggaa acagaccttg accttggaca
     1021 ctatgaaaga tttatcgacg aaaacctaag cgtaaattca aacgtaacaa caggtaaaat
     1081 ttattggtcg gttatttcaa aggaaagacg cggtgactat gagggaagaa ccgtacaggt
     1141 aattccgcat atcacaaacg aaatcaagtc aagagtttac cgtgttgcaa atacgcagga
     1201 cacagatatt gttattacgg aaatcggcgg aacagtcggt gatattgaat ctacaccgtt
     1261 ccttgaatca atcagacagg ttgtaaagga agtcggtaag gaaaattgta tgtatatcca
     1321 ccttacactt gtaccgtatc tttcaacatc gggtgaacaa aagacaaagc cgacacagca
     1381 cagtgttaag gaacttctaa gccttggtat tcagcctgac gtacttgttt taagaactga
     1441 aaagcctctt gaagaaggac ttgcagaaaa gattgcgcta ttctgtaatg tatcacctga
     1501 atgtgttatc gaaaatcttg accttgatac gctttacgaa gttcctcttg cacttgaaga
     1561 agaaggactt gcaaaggttg tatgtaaccg tcttggactt gaggacagaa aaccacagct
     1621 tgatgattgg acaaagatgg ttgacgtcgt taaagacctt atgaacggcg gcaaggaaga
     1681 agttacaatt tcacttgtag gtaaatatgt atcattgcac gacgcatata tcagtatcgt
     1741 tgaatcgcta aagcacggcg gaatacagaa ttactcaaat gttaatatca agtggataaa
     1801 cagtgaagaa gttactgcaa agactgcgga taaacttctt aaagattctg acggtattct
     1861 tgtacctggc ggttttggtg acagaggtat tgagggtaag attattgccg ctcaatatgc
     1921 aagagaacac aaaattccgt atcttggaat ttgtcttggt atgcagattg cggttataga
     1981 atacgcaaga aacgtacttg gctacaagga cgcaaattca tcggaactta accctgacac
     2041 aactcaccct gtaatcgacc ttatgcctga acaacgtgac gttgaagatt tgggcggtac
     2101 aatgcgtctt ggattgtatc cgtgtaaggt tacaccgaac acacttgcac aaaaggttta
     2161 taacagtgac cttatttatg aaagacatcg tcatagatat gaatttaaca acttctacag
     2221 aaatgaaata acagacaagg gaatggttat ttcaggtcag tcacctgacg ataaacttgt
     2281 tgaaatggta gaaattccta atcacccgtg gtatatcggt gttcagtcaa gacctaaccg
     2341 tccgcaccca ttgtttgcct catttatcaa ggcggcattg gacaagaaga ataaggcggc
     2401 tgaataattg gattgcctgc ttgacagaaa ggtaagatta acttgaaata ttattggaag
     2461 tatataaaac cgtatatgtt gtttttcata ttaggtcctt tgcttatgct aacggaagtt
     2521 gcaggggaaa tcgtgttgcc taaaattatg gcatcaatga ttgacagcgg tatcggtgcg
     2581 gaagtcgcag gacttggaac gggatatatt ttgtcgcggg cgttgcttat gattgcgtgt
     2641 atctgtatta tgataatcgg cggtatcggc ggtcatttct ttgcgattag ggcgtcggtt
     2701 tatttcagtg cggatttaag acagggagta tttgaaaagg tacagaactt ctcatttaag
     2761 aatatagaca acttttcgac aggctcactt gttacacgtc tgacgaacga cattacgcaa
     2821 gtgcagaata tggttagaat gcttctgatt atggcaatgc gttcaccggg tatgctgata
     2881 ggcggtatta taatggcttg ttcaattaat aaagaacttg cgcttatatt ggcattcgtt
     2941 attccgatac tggcggtatt gataggattg ctgttaaaaa ccgcgtttcc gcgatttggt
     3001 gcaatgcaga aaaaacttga taatcttaat tcgggtatac aagagaatat aacgaatgtt
     3061 cgagttataa aatcttttgt aagacaggat tttgaaacag aaaaatttga aaatgccaac
     3121 tcagacctta tgaatactac aatgagagcc ttgaaaatcg ttataacgac aatgccgatt
     3181 atgacgttgg caatgaatat aacaacattg cttgtggtat ggttcggcgg taattttgtt
     3241 gtaggcggaa gtatgggtgt tggtgacctt actgcgttta caacatatat tacgcaaatt
     3301 cttatgtcac ttatgatggt ttcaatgctt ttcttacagc tttcgcgtgc gcttgcgtca
     3361 tcgacaagaa ttacggaagt gcttaaaaca gatgtagacc ttacagatga aaatgcaggt
     3421 caaaaggata agaaagttga aaacggcaga gttgaattta aaaatgtttc tttcaagtat
     3481 tacgaaaaac gtaaagaaaa cgtacttgaa aatatttcgt ttaccgcaga gccaggagaa
     3541 attgtcggta taatcggcac aacaggctcg ggtaaatcgt cgcttgtaca gcttataccg
     3601 cgtctttatg atgttacaga cggtgaagtg cttgttgacg gcgtgaatgt taaggattac
     3661 ggtcttaaaa atttgcgtga cggcgtcggc atggtacttc agaaaaatgt attgttcagc
     3721 ggaagtatcg aagaaaatct tcgttggggt gacgaaaatg ccacaatgga agaaattcgt
     3781 gccgctgcgg acgccgcaca ggctgacgga tttgtaaacg catttacgga cggctataat
     3841 atggagctcg gacagggcgg tgtcaatgtt tcaggcggac aaaagcagag attgtgtatc
     3901 gcaagagcac ttttgaaaaa gccgaaaatt cttatacttg acgacagtac aagtgccgtt
     3961 gacacagcca cagaggctaa aatcagacaa gcgtttacaa cgagtttgaa acactcgact
     4021 aaaataataa tagcacagag aattacatcc gtaatagacg cggataaaat aattgtgctt
     4081 gaggacggaa agattgtcgg tatgggaaat cacaatgagc ttatgaagaa ttgtgaagaa
     4141 tatcgcgata tttactattc tcaaacagat aaagaaaaag aagtttctta atttgtacat
     4201 cttatcagcc cacgataaga aatgtattga ctatgtgggc agaaaggcta tggaagtaaa
     4261 atgcctccaa tgggaccacc gaacaggaac agaaatcaga aaaaacagaa acctaaaaat
     4321 acannnnnnn nnnctgcgtc aacattgtgc ggctcgtatc ttttaagacc gattattaac
     4381 ggacttatag attcgaccaa aacatctcag cagaaaatca cttcgcttat ggcaggactt
     4441 gcacttatgg cagtggttta tgtactcggt gtcggtgcaa catatttgca gggacgtatt
     4501 atgatcagcg tttcacaggg tactctgaaa cgtatcagag agcatttgtt ccgtaaggtg
     4561 cagaaacttc ctgtcagata ttttgacaca aaccctacgg gtgacataat gagccgtttt
     4621 actaatgacg ttgatattat cggcgaaatg cttaattcga ctcttgtaca gattttctcg
     4681 ggtacgataa ctttaatcgg tacacttgca cttatgcttt acacaaactg ggtgcttgcg
     4741 gttgtgacga ttgtagtatc accgattatt gcaaaaatcg gaactgccat agccggtaaa
     4801 tcgcgaaaat attttatgaa acaacaaacc gacttgggta aagtaaacgg ttatatcgaa
     4861 gaaaccgtta caggtcaaaa ggttgttaag gtatttaatt atgaaaaaaa tgtcgtaaat
     4921 gaattttccg agcttaatca aagtcttaga aattcacagg ttaaggcaca gtttataagc
     4981 ggaattatgg ggccttgtat gaacgcaatg agccaggtca actacacatt gacggcttgt
     5041 gtcggctcga taatcgcttt cgcgtcacgt tggggcggag gtgttccgtt ttccgcactt
     5101 gacataggcg gtcttactgt tttcgtaaac tattcaagac agttttcaag acctataaac
     5161 gaacttgcac agcaggttac aaatattatg tcggcacttg ccggtgcgga acgtgtattt
     5221 aacgtaatgg acgagacgga agaaatagat gacggtaaaa aacttgtact tgataaagta
     5281 cacggtgacg tcttggttga gggtgtgaca ttcggatata atcccgataa aacaattttg
     5341 aaagatgttt ctgttttcgc ccacccggga cagaaaatag cacttgtcgg atcaacaggt
     5401 gcgggtaaaa cgacaataac caacttgatt acacgttttt ataatatcaa taaaggaaaa
     5461 atcactattg acggtgttga cattaaggat ataagccttg aatgtttaag agaaaacatc
     5521 gcgatggttt tacaagatac acatttgttt acaggcacaa ttatggaaaa tatccgttac
     5581 ggcagacttt cggctaccga tgaagaagtt cgacaggcgg cgaaaacttc ttgtgcggat
     5641 atgttcataa aaaatatgcc tgaaggctat gatacaatgc taaaggggga cggttcaaac
     5701 ctttcacaag gtcagcgtca gcttttgaat attgcgcgag ccgctttgtc aaaagcaccg
     5761 atacttgtgc ttgacgaggc gacaagctcc gtcgatacaa gaacggaaaa acatatcaat
     5821 gagggtatgg acgctcttat ggaggacaga accacgtttg ttatagcaca cagactgtct
     5881 acaattcgta atgcggacgc gattatggta cttgaaaacg gtgagattat cgaaagaggt
     5941 actcacgaag aactgcttga aaagaaaggc agatattttg aattgtatac gggactcaaa
     6001 gaacttgatt aatttatgct atatggcata gaaaggaatg gtcagataaa atgaaggtat
     6061 atcagttaat ctatacctct gtacagcata gcctgtccga tcccgaactt ggacttgtca
     6121 atcagtcggg gctgagagtg ttttcatgca cacagggact tacaaagcaa aatattgatg
     6181 aaacaataag atttgctact tacagacttc cgaaaaacaa tgaaattaaa tatacacaga
     6241 caccttgcga tccgacagtg cctgaactgt ttccgaaaat tttcagaacg ttcagacttt
     6301 cggacggaag atatgttgca atgcagattt cttatgccgg ttatgatttt gacggtcaac
     6361 cgggcaatgt gtttgctcat gcatttatat ttgatgatgt tgacgaaaac tttttgccgg
     6421 aaagatatat cggacataag cgttacagaa cgcatcttac cgaaaaggat ttgaacggtc
     6481 agattgttca ttatttgaaa ccgcttgata atatcgcacc gtctgaaggt gtcgaaaata
     6541 aggttatcaa ctttatcggc gaacataagt atgaattgac ttatgtttta gacagagcga
     6601 caagacttct gacttcagat gatattaaaa atatatgtat cgcggcagat gacgcggaaa
     6661 cggtgcaaat gtatttgctt gcattaaagt ggattttacc aatcagtctt tccgaaaaca
     6721 caggtatttc aacgtataat gtgtacttac cgtcggataa acaaaaacag attgtgttcc
     6781 acggaactat aaaacagcat aataacataa caggtcaagc cattgaggcg agaaaaaatt
     6841 gtatctatat tgatatagaa
//
LOCUS       DAWACS010000028         6739 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1290_length_6739_cov_1.996858, whole genome shotgun sequence.
ACCESSION   DAWACS010000028 DAWACS010000000
VERSION     DAWACS010000028.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6739)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6739)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6739
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1290_length_6739_cov_1.996858"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2705..2714
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3199..3208
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4128..4149
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4702..4711
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5600..5609
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2154 a         1106 c         1569 g         1848 t
ORIGIN      
        1 tgccgttaaa aaacgtggtg acgatattac gtttttgcgt aaaatcgtca gaggcggtgc
       61 ggacgacagt tacggtattg aagttgcggc acttgcaggt gttccgaaag aagtaatcaa
      121 cggtgcaaag aagattttga aaaagattga gaatgatgaa atcgttgtag aacatacggc
      181 gaagaaacac gaggacgcaa caaatcaaat cggtttcggt gacggtatag caaatgaaat
      241 agcagatgaa cttaaaaata tggacgtcac aacgtacaca ccgattgagg cgatgaacaa
      301 gctttacgaa ttggcaaaca gggcgaagga aatataaaca tttgtggctg ttaaggtcac
      361 gaatattaac ggttgaaagg gtttaaaatg ggaaaaattc atgtactgag cagtgaagtt
      421 tcaaataaaa tagcggcggg cgaagttgtt gaacgtccgt caagcgttat aaaggaactt
      481 gttgaaaaca gcatagatgc cggtgcaact ctgataagcg tggagataaa aaaaggcgga
      541 agcatatata tgcgcgtcag cgacaacggc agcggtatgt cggaggagga tgcgaaaata
      601 tgctttttac gccatgcgac aagtaaggtg cagacgagca gtgaccttga cgcaatttat
      661 acgcttggtt tcagaggtga ggcactttca agtataggtg ctgttgccga agttgaactt
      721 tttacaaagc gacacgagga tgaaacaggc gtatgtgtaa cgtgcaaagg cggagaaata
      781 ctttcttctg acgaggcagg tattcccgac ggtacatcga tacttgtcga aaatctgttt
      841 ttcaacacac ctgcaagacg caaattcttg aaaaaagacg caactgaggc aggatacata
      901 acggatataa tgacaagatt tatattcgca catcccgaaa tatcctttaa gctgataatc
      961 gacaacaaag aaaaattgtt ttcaccgggc gataattcgc ttgaaaattc ggtttataca
     1021 gtttacggca gagattatgc aaagggtaca attcctgtag aatacgaatc ggacggcatt
     1081 aaaattacag gtcttatcgg caagggaact cttgcacggc ctaagcgaaa ttatcagagc
     1141 ttttttgtaa acagaagata cataacaagc agaacaataa tcgccgcact tgaaaatgcc
     1201 tataaaaatc agattatgat aggcaaattc cctatggcga tacttaatat agaaatcaat
     1261 ccgtcactta ttgacattaa cgttcacccg acaaaacttg aagtgaagtt ttcagatgaa
     1321 aaggcggttt ataatgcggt gtattacggt gtgaaaaacg cactttacga aataccgaat
     1381 gtaccgaaaa tcgagcgtac ttcggaagaa tttaaacgtg atacgccaaa gggacagtta
     1441 aatctaagcg acattgcggt tgcacttccg aagacaatga caaaacgtcc ggaaacgacc
     1501 gcatataatc cacgagaaaa tcatttcctg aaaaacagtg gggacactaa aaataattcg
     1561 gtgagtgaca ataaaacgga taaagttggg gacactaaaa cacaaaataa ttttgtatca
     1621 ctaaaacccg attttataaa atcaagacaa cccgaaattc cttatacaag aactgtatcg
     1681 cttgaaactc cgaaggcgga aacaaacgga atacttccta aaaaggacgc ggttaaagcg
     1741 gaagaagcca cagttgaaga aataaagaat gaggaaaaaa ctgttactga aacattaagg
     1801 gttgaaaccg ttaaggaaga aaagccgaca gagcaaaatg ttgaaaccga agttccgaaa
     1861 cgcgaagaaa ctgtttttgc agacgaatat tttgaaatag tcggtcaggt ttttgattcg
     1921 tacatcatag ccgaaaaggg cgacgaaatg atgattatag accagcacgc ggcacacgaa
     1981 cgtttgaaat atgaagaatt gaagaaagat attgcgtcaa aacaagttgt gtcacaaatg
     2041 cttatcgagc ctgttatagt aaatcttacg ggcagtgaaa tgactgcgta tcgtgataat
     2101 aaacagcttt ttgacgatat aggctttgag tcggaagaat tcggcgatga cgcggttatt
     2161 gtacgaagtg ttccgggtga agttgaactc ggtgaggttg aaccgcttgt gcttgaactt
     2221 gtcgcacaag gcgaggaact gaggaaagag cttataacgg aaaagaacga aagactttta
     2281 tatacgattg cgtgtaaatc ggcggttaaa gcgaatatgc gtatgagtaa aatagaaatg
     2341 gaaacgcttg tgagaaatgt tttaaggctt aaaaatatta atacttgccc tcacggcaga
     2401 cctattatag taacaatgag caaaaaggaa cttgaaaagg agtttaagag aattgtctaa
     2461 gatacctctt attgttgttg tcggaccgac cgcgtcggga aaaacggcac ttgcgattga
     2521 tattgcaaat tatgtcggcg gtgaaatcgt cagtgccgac agtatgcaga tttataaata
     2581 tatggatata ggctcggcaa aaccgactgc ggaagaaatg agtcaagccg ttcatcatct
     2641 tatcggtttt cttgaacctg atgatgagtt cagtgttgcg gactataccg agcttgcaca
     2701 taagnnnnnn nnnnaaattg caaaagacgg cagaggaaaa aggcagtgaa tatttgcttg
     2761 atatgcttgc ggaatttgat aaggagtctg ctgaaagact gcaccctaat aacgtcagac
     2821 gtattattcg tgcgatagag ttttataaaa cgacaggcaa accgatttct aaacaccaag
     2881 aggaaacaaa gcgagttgaa agccgatata atccgcttat gatttgtatt gattgggata
     2941 gggacgtact gtatgacaga ataaacagac gtgtcgatat tatgataaaa agcggacttt
     3001 tggaggaagt taaaggtctt atggatatgg gctatacgaa ggaattaaat tcaatgaagg
     3061 gtataggcta taaagaagtt atggactatt atgacggtaa aatgagcctt gaagatgccg
     3121 tagaactcat aaagcaaagc ccccgccgtt atgccaaacg tcagcttaca tggttcagac
     3181 gtgacgatag gatacattnn nnnnnnnntc tttctgttct tgacgcgttt ctgtttgggg
     3241 tgcgtgtacc tgtttcattt tccgatgatg aacgctgtct gtttttcttt aactgctgaa
     3301 tacgtctttg tctttcacgt ttcttcttct gatatcttgc gtacataata agaagtataa
     3361 gaagtattgc cggaatgaag aagaacggac ttgttataac ctttaacaca aggtggaata
     3421 cgtgaagaat attatttctt tcaacatcat ttgttgcgat aaggttagcc gaaccgactt
     3481 caataccttt atatgaatat gtcactgtac caagtacgtc accttttgta ataggtgcct
     3541 ctaatttttc gggcataaac ctttgaatcg gaaactatat ctccgactgc cgcaagcggt
     3601 tgatgtgtgt aattatcaaa accaaaattg aacaaagcct ttgaatcaat atatgaataa
     3661 gcctgttcct ttgtatctac gtttttacag ttcataacga ttgcaagaaa ttcggtatcg
     3721 ttatatgaag cggctgatac aagacagtat ccggcttgtg aggtgtgacc tgttttaata
     3781 cctattgccg gtgggtaata gtagtaagtc gaacgtgatg ttccgaggaa caggtttgta
     3841 tttacaagaa tacgctctga tgcatactta tttgttgccg gaattttgta tataggcatt
     3901 tttacaattt cacggaattg atcgtttttc attgcgtact ttgaaagtat tgcaaggtcg
     3961 cgagccgttg tatagtggtt atcgtcatgt gaaccgcaag gatttacgaa gtgagtgcct
     4021 gtcattccta attcctgtgc cttttgattc atcaaatcga caaatgcgtc cattgagcct
     4081 gcaatgtgaa ttgcaataac atttgccgca tcattggcac tgtatacnnn nnnnnnnnnn
     4141 nnnnnnnnnt tcctcgccgg taagtatacc catatgagaa tcttcaagag ttattgattt
     4201 cagcgcatca tatgtggcgg ttaccgtatc gtccatatta cccatttcaa gtgcaagtat
     4261 agctgtcata atctttgttg tacttgcagg gtaaactctt tcatcaaggt ttttgctgta
     4321 taaaactcgt cccgacgcca tatccataag aagtgcggac tgtgcatact gcacgttcgg
     4381 aggcgtaagt gacggatctt ccatagttac gccgtccgga agtgaatttg cgttttgcga
     4441 tgttgtatct gtatcgcttt gcaaagttgt atcagatgtg cttgatgtat ccgatgaagt
     4501 atctgacggt gttttggttg cggatgtcgt ttccgtatcg cttgatgtat ctgtgctttc
     4561 ggttgccttt ggtgttgatt ttacatcatt gccgtcggaa gttactgttc tggttgttcc
     4621 gtcaggtgaa gttacggttg aagttgtgtt atcagtagtc ggctccggag tcggtgccgc
     4681 aaaagaaaat gatagcggca tnnnnnnnnn naaatgttag ttttgaacaa aaaatcaaaa
     4741 atattcttat acataatcat ttttgcattg ttggttgtgg gcggatttgc cgttgaacac
     4801 tttgaaaaag acgcttttgt tatggaaacc gtggcaacgg aggattatat tccgtatgaa
     4861 tcggacacga aagaagtcac cgacgggaaa atcaatatca attccgcaga tacagaacag
     4921 cttgtaaagc tgaaaggtat cggagagaaa atgtctgaaa gaattatcaa atacagacag
     4981 gaaaacggtc cttttatgtc aatagaggag ataatgaagg tgtcgggaat aagtgagaaa
     5041 aaatttgaag atattaagga atatatttcg gtagaataaa tctgtttttt aattaatata
     5101 aatattacag tgagttgaaa ggatgggaca gatgaaaata ttagtggtag atgacgaaaa
     5161 acttttggtg aaaggtataa agtttaacct tgagcaggac ggttatgcgg tagtgactgc
     5221 atttgacggt gaggaggcta ttcgtcttgc acatgatgaa agcatagact tgattttgct
     5281 tgatttgatg ttgccgaaag tggacggatt taccgtatgc cgtacgataa gaagtttttc
     5341 aaatgtacct ataattatgg ttacggcgaa aagtgaagat atagataaga tactcggact
     5401 tgaatacggt gcggacgatt atataaccaa gccttttaac attcgtgagg ttacggcaag
     5461 aattaaggcg attttgcgca gagtaaatcc tgcgccgaaa ggcgaaaggg acaagcttat
     5521 catatcgggc gatataactt tggactataa tttcagacga gttgtgataa acgataaggt
     5581 tattgaactt acaagaaagn nnnnnnnnna tttgattatc cgggtgatgt acgaacggtt
     5641 gacgttcata taagaaggct tagagaaaaa attgagaaaa atcctgccga gccttcatat
     5701 ataaagacaa agtggggagt tggttactat tataagaagg attaaggaaa agattaaaaa
     5761 catcaccaat tcaatgcgtt ttacgatgat tgcaacttat cttgcaatta ttgttacaac
     5821 gcttgttttg atgagtattt atataatcag tctgcttagc gagaatctgt acagtacgga
     5881 aacgataaat atgtttgcaa aggcgaatat tatatctgaa accattgcgg acgtatgggg
     5941 aagcgataca tctgcaactt cggctcaaaa gttttcggat gttgttgagc gaagccttgc
     6001 aggtacgaat ataagaggtg ttgtgacaaa taagtcgtat tcggttttgt atgacacgaa
     6061 tgaagaatca cagcttatcg gcaaggtttt tatgcgtgac gttttaaaac gtgcgcttga
     6121 cggcgaacaa gccgatatta taacgacagg cgaaaacaat atgcgtctta taagcgttgc
     6181 ggtgcctgta acattcagcg gtgaaataat cggaagtgtg tatttggcag aatcgataaa
     6241 cacgattgac gatacagtcc gaacgacaag gaacagtctt atagttttca gtacgcttat
     6301 tgtaattctt atcggtatgc ttagtttggg tatgagctat attataacgt caccgattga
     6361 agaatttatt gccgttgcaa aggaaatttc aaagggtaat ttctcaaagc gtgttgaagt
     6421 taaaggtcat aatgagattg cacaaatggc acaaatgctt aattatatgt gtgacgaact
     6481 tgaacttttg gaagaaaaaa gacgtaagtt tgtttcggac gcgtcacacg aacttaaaac
     6541 ccctatggcc ggtataaaac ttatatgcga cagtcttgtt gcggcagaaa atcccgatcc
     6601 ggctatggta aaggaattcc tcggcgatat gtcggacgag gttgacagac ttacaagaat
     6661 tgtcgaaaga ctgcttgtgc ttacaaaact tgacgcggga ggtaatccgc tgaaacttga
     6721 agaagtcgat attaagatg
//
LOCUS       DAWACS010000029         6628 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1313_length_6628_cov_2.470409, whole genome shotgun sequence.
ACCESSION   DAWACS010000029 DAWACS010000000
VERSION     DAWACS010000029.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6628)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6628)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6628
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1313_length_6628_cov_2.470409"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    219..238
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1938..1947
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6244..6253
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2141 a         1036 c         1643 g         1768 t
ORIGIN      
        1 tgtgattgac ctgttctctg cggaaacagg tcatgcgccc acggagatat gcgaatttct
       61 cggcagtaaa ttgcctgtgg atattatcga aaaaatcaga gttgaggtag agacacgtat
      121 tataaatcca tttatagaag aaattttccc ttgggaaaca tttaatcata attgggctgc
      181 ggtttgcggc ggagctgtcg gaatgacatt tctttatgnn nnnnnnnnnn nnnnnnnncc
      241 tgaaaaattt aatctggtag agaaacgcat taacggtgcg cttaagtcat acctttcggg
      301 ctttggagac gatggaattt ctactgaggg cttaggttat tggaattatg gattttggta
      361 ttttacaggc tttgctgatt tgtataagga acgctgcggc gtggatctta tgaataattc
      421 aaaggtgaaa aatattgcta tgtgtcagca gaatatgttt ttgacggaca atagtgtaat
      481 aagctacgga gattgcgtaa gacacgaaca gtatcattcc ggacttgcac attatctgag
      541 aaatcgttat ggcagcgata taagaattat aaatgacaaa atttcgtcag gagtagacca
      601 ttgctacaga tttctttcct gcacgagaac ctttatgtgg actgataaaa acttggcggg
      661 actcaaagaa gccaaaccat cagaaagcta ttttaaggat gcggaatggt atataaatcg
      721 taaagcaaaa ttcgcattta cggcaaaaag tggcagcaac ggcggaaatc ataatcacaa
      781 tgatgtcggc agttttataa tcgcgggtga agaaggacag cttatagcgg attttggttc
      841 aggcgaatac actcacgagt attttaatga aggacggtat gattaccttt gtaattcttc
      901 aagaggacat aatgttccta taatagacgg caaatatcaa tccgccggac agagtttcaa
      961 gggaaaggtc atatggaatg gagctggagt ttttgaactg gatatggcag aagcgtatgc
     1021 tggtgtcata aaaagactta aacgaaaatt caatctgaat gataactata ttgaacttac
     1081 cgacgttttt gaatttaatg atgactcaaa gcatgatata acagagcgct ttgtttcggt
     1141 aataaagcct aaaataacag acggaaaagt tactataggc agtatggtga ttgaatgtga
     1201 tgaaactccg atactaggca gtgagcattt gcaaaatcat gcggcagagg acgatgtgct
     1261 ctattttgta gattacaaat caaatacaac ttttaaaata aaatttatta tgcaataaaa
     1321 aagcggatgt tgaagtgaat tactcaaagg aaatacgcat actgctttta tgatatgcgt
     1381 atcttttttt gtaattgtta tatatgaaat aatgaataat atctgtgcat ttgatataaa
     1441 aaatatataa tggtataata tcaatataac aaatataaca aactcacata aatgttttga
     1501 aaatattcgg aaaggtggta taacaatgag taaaaacata catataatat cacattcaca
     1561 ctgggacaga gagtggtatc ttccttttga ggaacataga atgcgattgg ttgagcttgt
     1621 tgacaagtgt atggaactgt ttgaaactga cggttccttt aagagctttc atctggacgg
     1681 tcagactata gtgattgacg attatctgga gattaagccg gaaaacagag ataagttaat
     1741 taagtatgtc aaagagggca gatttattat aggtccctgg tatgttctgc aggacgaatt
     1801 ttatacaagc ggagaatcca acgtaagaaa tctgcttata ggtatgaaat ctgctgaaaa
     1861 gtacggcggt atgtgtaaaa tgggttattt ccctgacgcg ttcggcaatg cagggcagat
     1921 gccgcagatt ttaaaacnnn nnnnnnnaga cggttacttt tggcagaggt gtgcgtcctg
     1981 ttggttttga taacgaagtt caggcaaatg gcagctatga atcaccatat tctgaaatga
     2041 tatggcagtc gccggacggt acgcctattc tcggaatttt atttgctaac tggtataata
     2101 atggcaatga aatcccgaca gatgtggaaa ctgcaaaggc atactggaat gagcgcatta
     2161 agaatgctgc gacatttgcc tcaacggatg aatacctgat gatgaacggc tgcgatcatc
     2221 agccggttca aacggatata ggtgaagcta ttgaaaccgc gtcaaagtta tatccggaca
     2281 taaacttcat acattccaat ttccctgatt atataaaagc attaaatgat aatctttctg
     2341 atgatttaac ggtagtaaaa ggcgagctta caagtcagga taccgatggc tggacaacgc
     2401 ttgttaactg cgcatcctca catgtatatt taaagcgcat gaaccgaaaa tgcgaatcgg
     2461 cgcttgaaaa tgaagctgag cctatatgcg ttatatcgtc tctttttgga cagagttatc
     2521 ctgctgacga gcttgaatat tcatggaaaa cccttatgca gaatcatccg catgatagta
     2581 tttgctgctg cagtgttgac gaggtacagc aagaaatggt aacgcgcttt aataagagca
     2641 agcaagtggc tgattatctt ataagtgaag gaaagagcta tatagcagat aaaattgata
     2701 cttctgtgtt tgaaaaatat tgtgaggcta taccgttctc ggtatttaat acaactggca
     2761 gagagagaac gggcgttata tcggtggatt tggatatagt gcgtaaatca ggttggctta
     2821 aaaaatgtgc atatgaaata gacgagatta ccgtacccga atacaaactt attgatgcgg
     2881 acggcagttc aattccgttc agacttgagg atttaggtgt aacatttggt tttgatttac
     2941 ctaaggataa gttcagacag ccgtatatgg caagaacggt gcgtataact tttgaagcag
     3001 agaatgtacc cgtaatggga tatagaacat atgcgcttgc agagggcagt gatatagata
     3061 caaaggaaac acttgtcaca ggcgaaaacc gtatggaaaa cagtgtaata agcgtgcaga
     3121 taaacggcaa cggaactctc gacataaccg ataagatgag cggcagaata tatagaggtg
     3181 tcggttatta tgaagaaaca ggcgacatag gtaacgagta tatgtataag atgcctgaag
     3241 gaacagagcc gataacaaca aagggagata atgctaaaat tgagcttgta gagaacgaac
     3301 cgttcagagc tgtttataag attacaaata caataacagt tcccgaagca ggcgatgaag
     3361 catttgagga cgaaaagcgt cacatggtat tttttaaaga aagaaaaggc ggaagaagta
     3421 aaaatactgt cgagcttaca atagaaactt atgtatctct tgataagcat ggaaaaggcg
     3481 taaaaataaa aatagtattt gacaataggg caaaggatca tagagtcaga ataatggtgc
     3541 cgaccggtat agactccaag gtacatagtg cagattctgt atttgagctt gttacgagaa
     3601 ataattgtca taatgcttgc tgggataatc cgagcgcctg cgagcatgaa cagggatttg
     3661 tgagtataga cgacggcgaa agtggcatag ctgttgctaa tattggtctt tatgagtacg
     3721 aaatgctgcc cgacatgaat aatacgcttg caataacact tctgagagct acgggcgaaa
     3781 tgggtgattg gggagtattc ccggcacctg aggcacagtg tataggcata agcgagacag
     3841 aaatagaaat tgtgccgttc agcggaaata ttataaacag caaagcctat gaagaatgtt
     3901 atcagttccg tgtggatatg cctgttgcaa caacgagctt gcataaaggc agtatgccgg
     3961 ataaatattc aatgcttgac tggagtggag aaggtttgac gcttacagga ctaaaagcaa
     4021 aacaggattc tgatgatatt attatcagat gggtgaattt gagtgataag cctactgtat
     4081 tgacgataaa gaagacagaa gtaataaaca acctatatca gagtaatatc atcgaacagc
     4141 gttttacgga tattgccgca aaagataacg aatactttaa tatcgaggta aaaccgtatg
     4201 agattttgac agtcggagtt gagagaaagg aagcgtaata atgtatatag gaatagacct
     4261 tggcggaaca aatatagccg ccggcattgt aagagaagat ggaaaaattg ttgtgcagtc
     4321 ttcagtaccg acgctttctc aaagacctac agatgaaatt gtaaaggata tggtattttt
     4381 gtcaaaacag cttataaagg atgcggagct tgaattgaac gatattgagg ctgtcggaat
     4441 aggctgtccg ggaacaatta actttgagac aggcgaggtt atttattcaa ataacataaa
     4501 aatggagcat tatatgcttg cgaaggagtt tcaaaaatat cttaatttgc ctgtcaagat
     4561 tgataatgat gcaaactgtg ccgctatggg agagtatatt gtgagtggta acaatgttcc
     4621 tatatttatg tttataacgc tcggaacagg tgttggcagc ggactgattt tgaatgggaa
     4681 ggtgtttcgc ggttttaacg gagctgcgag cgaagcaggg catattactc ttgtttcggg
     4741 cggagaaccg tgtacttgtg gaaaacgtgg ttgctgggag acgtatgctt cggtaacggc
     4801 gcttatacgt cagactaaag tagcaatgga gaaaaatcct gaaagcctta tgcatgaaat
     4861 cgccaaggca gaaggcaaaa tttcgggcag aacgtctttt gatgcggcaa agcagggtga
     4921 caaagctgcg caggctgttg taaagcagta tgctcagtat gttgcggatg gaattgtgag
     4981 cgtagagaat gtattgcagc ccgacattat atctgtaggc ggaggaataa gcagggaggg
     5041 cgagtatctt ttgcagcctg tatgcgaata tgcagcagca aacggcttca ataaatttat
     5101 gcctaaaaca aaaattgtaa cggcacaatc ctttaatgac gcgggaataa tcggcgcggc
     5161 tatgattgca aaataaatat tgggaaaaga agatgccgat aaaatacttt cttttctatt
     5221 caaatttaga aaggggacat atctaatgaa aaaaataaca aaagtgattg cggcggcaac
     5281 agcgctcagt gcagcgcttg gagtatgcgc gtatgctgcg gaggatacga tagaggataa
     5341 ggttatagct ggctatcagg catggtttaa cacggaagga gacggttcac cgagaaatga
     5401 atgggtacat tggggcagac cggaggttgg acatttgacc tttgacgcgt atcctgatgt
     5461 aagagattac tccgtggagg acttgaaaga aacatatttt gcagaacttg gaaacggtga
     5521 aaaatcaaag ctgttttctt cgtatcgcgc aggtgttata gataagcatt ttgagatgat
     5581 gcacgattac ggcattgacg gagttgcggt tcagcgattt ggttcggtta caataaacgg
     5641 tgttgaggaa agatttaaaa acagcgttga tatactgaaa aacgtaaagg catcggcaga
     5701 aaagcataac gagcttttct atgtaatgta tgatatatcg ggtcttagcg aggaaaatct
     5761 cgtagaagat ttaaaggcgg attggcagca ggtgattctt aacgacttgg atattacctc
     5821 ctcgccgtca tacgcaaagg ataatggcag acctgttgta tgtctgtggg gtttgggaat
     5881 acgcgaaggt actggagaac agtattccga actgatcgat tggtttcacg ataagggctg
     5941 ctatgtaata ggcggtgttt caaagagctg gagagatcag atagcgaagg acgaagctat
     6001 ggagaaagca ttctgtaagc ttgatatgct gtcaccgtgg tctgtcggca gtgcaaaaac
     6061 agacagtgat gttgacagtg atgcgagtat tcttgcagcg gataaggcat atcttgacga
     6121 aaagggtata gcgtatcagc ctgtaatgtt tcccggattt tcgtggtcaa actggaagga
     6181 taacgcggta agaaatcaga taccgagacg cgcaggggat ttcatgtggc gtcaggcata
     6241 taannnnnnn nnnatattgc aaagcaagat atacactgcg gatacattgc gatgtttgac
     6301 gaatatgacg agggtactgc aattttaaag cttgcggagg actcgtctat gattcctaag
     6361 gatcaatatt ttataacagc aagcgcagac ggaagttaca cttcatcgga tttatacatg
     6421 cggcttgcgg gatatattcc aaaagtgctg cgcggcgaag aggaaattac agaagaaatg
     6481 ccgctgctgt taagtgaagg tcctgtttat ttcagaagcg gttttgagaa atattttgat
     6541 gcgcagcctg tagatggcaa cggtgagctt gtaaaggatg aacttacata taacggcaga
     6601 tatgccttga agcttaatag agtgggcg
//
LOCUS       DAWACS010000030         6547 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1330_length_6547_cov_2.351356, whole genome shotgun sequence.
ACCESSION   DAWACS010000030 DAWACS010000000
VERSION     DAWACS010000030.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6547)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6547)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6547
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1330_length_6547_cov_2.351356"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3271..3280
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3912..3921
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2198 a         1146 c         1314 g         1869 t
ORIGIN      
        1 atttcccata caatttaaca gtatttcaac ttggtcgcta acttggtcgc taacttggtc
       61 ggtggtgtct aaatcattta gggcatcgaa tattatttgc atcatcattt ctacaaatac
      121 agtacagtca gctttgttat ctgagatagc gagagcgtca taatattctt gttgtcgctc
      181 ttttataagt tcttcaatag gtaaccaaga gaataattta ttccattgac taagtaatag
      241 tgtgtgccac attctaccga ttcgtccgtt gccatctgca aacggatgaa taaattcaaa
      301 ctcatagtga aaaatacaac ttttaattaa aatatgcaat ggagattgtt tgtaccattc
      361 aaataggtcg ctgattaatt ggggtacata ataccgacac cgccatttct aaaacgacca
      421 ttttctttaa ctaaatcatt catcattagc ttatgtgctt ttaacaaatc ttcaatacta
      481 agaggattga gcgtaagcat tatatcataa gcttcatatg cgttttgcac ttctttgatt
      541 tcttgaggtg cacccaaaat tcgcttacca tttataaccg ctgtaacttg ttctaatgat
      601 aatgaattgt gttcaatagc cagagatgag tgaattgttt ttattctgtt ttcacgtctt
      661 agctttagac tgctactttt atgcaatata cttacttgtc cgacaagttc actggttttg
      721 gcaagtaaat ttattatatc gtcattaatt gaaaatggtg gtttgtattc catttagcat
      781 acctgccttt ctataagcta tttacaaata ttatatcata tttgtttcat attttcaaat
      841 caatataaat tttagattga agtatgttat cagatgttcc tttggcatat agtgtattaa
      901 caaactcacc tatattccaa cactttaaca tattctgaaa aactataact gctgttgtta
      961 aagctttggt tcatgataac cataggagat taatattttt gtggcaaata caaccgttcc
     1021 gatatatttc tgttatgatg tctgaatgct atttttggtt gtcattacac tttatcatct
     1081 cacgcggtgt acgacccgta aaatgcttaa aattgcggtt gaagctgcat aggctgtcat
     1141 agccgcactc ctccgcaatc tgctgtatct gcacatgatt ttgattgctg agcatataca
     1201 atgctcggtt tattctgtat ttattcaaat actccgtata acttattccc atacgttttt
     1261 taaaggttct cgatagatac gaatacccat aagacaattc ttcggcggct gtctgcaaaa
     1321 ggcagggagt tttaaagttc ttttcaatat atcgtaaaat tttaatgagc aactgtgact
     1381 cctcattatt tgcggcttct ttaaaattcg tactctccac taaaattccg aatatagaat
     1441 ataacaagcc cttttgctca aaaatatttt tcgcgtcaaa atattttttg aaattcgaaa
     1501 tctccttaat atcatttttt tcgggaagca tattttgata gcggtttgta aattcgggga
     1561 ttaaatcagg cataaaagtg attacacgca tttttgagct tttttccgtt tcatagctat
     1621 ggagctgacg tggaaatatt attacgctgt cattctgttt taaattatat aatacggtat
     1681 ctattgtaat atttatttcg ccgtcaataa taaagcaaat ctcaaaagac tcgtggtaat
     1741 gtatattcca attcagatta tccacatcat atgaaagaaa tttgtaattc gggttttccc
     1801 attctttttc gtacatcata aaaatcacct gttaaaagta tatcatttca tatactctcg
     1861 gtcaagatta aagagcaaaa attgattagt ttacggcaaa tatatatttt tacctatata
     1921 ttattatatg ataaaatact ataataaaaa taatatttta acggaggtag gaaaatgata
     1981 ttgaaaaaat tgataagtgt aatgagcgta tttgctatta tcgtatcgtc atttaccaca
     2041 gcagttgtca gcgctgacga ggaaatgaca ccgattgcct gtacgcaaag cgcgtcatac
     2101 ttgacggacg gtgacggaaa tgttacgcag acgatagtga actatgcggg caaggaggat
     2161 atttcaaata ttaactggaa taccaaatca aattcttggg gcggagtggg cgttcttgaa
     2221 tttaccgttc ctgcggcaga agcgaaacgc ataaaatccg ccacacttac cgtaagcgta
     2281 cataacggct cgtcaagaag cggtggcaga acatatgata tatatccagc cgacatcaca
     2341 ataaacgccg atacaacggc ttccgaaata aaggcgattt cacttgacaa aagtatgtat
     2401 caggcggagg gggttaaaca gggcgaaacg cgcacggacc aaatatcaac cgaaacaatt
     2461 cgtgaatatg taaaaggcaa ggtaaaagca gatgtggaaa gcaaagtaca attcgcgttt
     2521 tcaaactcgt cacagacgct tgacatagac ccgaaaaccg cgacgctcgc acttacaatg
     2581 tatgacggcg gcgttgcact tgataaaaac gagcttattc tatctacctc cggcgagcct
     2641 caaagactta cctactctat tttcggagag ggaataaatg atggtgacct tgtatggata
     2701 agcgaaaatg aggatatagc caccgttgac gacacaggtc ttgtcacacc gaaaaaggca
     2761 ggcagaacag tggtgtctat aaaaacggct gacagcagtt tcaaagccga ctgcgccgtt
     2821 actgtattgc aggcggctca ggatataact attgacaaag cctctctctc actgctatcg
     2881 ggcggtaaaa acggcgaact tacagccaaa ttgctgcccg aaacagcagt taaacgacaa
     2941 ataaaatgga cgtcctcaga tgagaatgta gcaactgttt catcaaacgg tattgtgact
     3001 cctataagcg ttggtgaaac aataattacc gcagcggcgg ctgacaacga aaagcttacg
     3061 gcttcatgca atgtaacggt agcggaaatg gctgaacctc aaagcatttc acttgataaa
     3121 gaaaatgtat ctttaccaaa gctcggtgca acaaccgtag taaatgcaaa tattcagccg
     3181 tcaaacgcgg atgacagaat tacttggact tcaagtgatg aaaaaattgc aaaggtttat
     3241 gatggcgtta ttgtcgcagg cgaagtcggc nnnnnnnnnn cggaaataac agccactaca
     3301 tcaaatggca aaacagcgaa atgtactgta acagtaacag aagataagca gcttataaca
     3361 aacgaccgtt tctataccga tacagacgga aatatcctat attcgcaagg tggtggtata
     3421 ttcaaattcc ctaatgatga taaatattat tggtacggcg ttcgctataa agaggctgta
     3481 acgtatgcga ctgaccctct tttaggtaaa acggttgagc atcctgcttt tgaagcatac
     3541 acttgctaca catcagatga tttggtaaat tggaaatatg aaggcgatgt cgcaacgctt
     3601 gaaactctgg gtcaatcatg gtgtggttgg gcaggcagat gcggtgtggt atataacgaa
     3661 aaggcaaata aatatgttct tgtatcacag tttaacggaa cgattatcgc aagtgcggat
     3721 aatccgaaag gcccatttaa aacagaaaag ggatattttt ggggtggaac gtcacttcct
     3781 gttatcgcaa atggtgatac aggtgattta acaatgttct atgatgaaga cagcaaaggt
     3841 tatatgatat gttcaagcgc gaacggcaga ggacatttat atattgcacc tatggatgaa
     3901 actaagaatt tnnnnnnnnn ngcggtatag agggtgactg tatgtttaca tacaaagacc
     3961 attattattt taccggttca gatttatacg gctggcgtgg ttcaagagtg tatgtgtttg
     4021 aatcaaaaga tatactcggc gattacaaat taaagccgga ttatattgat acttcaaaat
     4081 caagttcaaa tcttccgtac attatgccgg gtgcaaagta tagctatgca cacaattcac
     4141 aaaccggatt ttactatacg cttcacggaa gcaagcagga cacggttatt tattgtggtg
     4201 accgttggtg tgatttcgga acacatggta taggatataa tcagtgggtt ccactgacaa
     4261 tggacggata tactcctcat ttcaacgatt taagtcaatg gaaattaaac tcagaaacag
     4321 gcgagtggac aatcggcgac ggaaataatt atattgcaaa caatgaattt gatgccgaca
     4381 gagttgatgt taaatcattg acaggttggg aatgcagtga cagtacagac ggttcggtaa
     4441 acgggaatgt aaaagcaaga cgtttttacg gcaactatgc cgcaaagcaa agtgcagaca
     4501 cagattatac cgcaagactt aaacaaacaa ttaaagattt gccaaacggt atatatacac
     4561 ttcgagcaag cgttatgtca agcggcggac aaaatgaatg tgtattgtat gcaaatacaa
     4621 acaataagaa ctacacagct tctctaaaat caaaaatgag taattggaca gatgtcgttg
     4681 taaaggatat tattattgaa aacggagaat gtgagattgg cttgtactca gactcaccgg
     4741 caaactgtta tgtaaggata gacgatatgt atttaacaag aaattatgac ggtacggtta
     4801 tcgagggtag gttaaatact aatgttccgg atgagcatat tgaagattca attacactga
     4861 aagatatgca gggcaacgca gtaaataagc ttaacggcgc agaggtatat gccgaggcag
     4921 tatatacaaa cgacaatgca gacagaaagg aattaacatt gtatatggct ttgtatgata
     4981 actatggaaa attaaaatct gtaaagcaaa gaaaagaaac aatcgctgca tacagcactt
     5041 ccacgataaa gacggacagc ttaagtgcag atactgactc atatatcaag ctgttcttgt
     5101 gggaggacgg aatgaagccg ttatataatt caataacaat taaggaggca aagtgatgaa
     5161 aaataaactt aaaagaattt taggagtgat aacagcaata tcgcttctac tgcaaatgtc
     5221 atttgttata acctcaaacg cggcggaata tttttcgctt gattttgaaa gtgcaacgga
     5281 tacaatgggc tggacgtctg caaatgctcc gggcgatatg tcaatagcaa cagatgaaga
     5341 caacagtatc aataagtatt tcaaatttgc aaatacaaac ggaagtggaa cacgaaatgc
     5401 atactatacc ttcgattctg acgctcagac aacggaaaat ggtaaagcag ttatcgaatt
     5461 tgacttctat atgacaaata caggcaatga aaatataaat cagcttgtat tacttgattc
     5521 tgcagcaggc aaccctaaag gcaatgcaaa ttacagcgga aataattata tcttttcatt
     5581 tacacaacct aagaatagcg ataaactcat cataaataat ttagatggtt ctaaggaaac
     5641 atatacaaca acagattgga cgaataaatc aggctggaca cacgcaaagg cgattatgga
     5701 ttttacagag cataatgtga caatcacatt gtcaagtcat gacggtgaaa cggtatattt
     5761 tgataacaag gtccctatgg gtacgacaga cttaaaaata ggaaaattac tgatttgtga
     5821 ggcgagaaag aatactgtga cattcggtat tgataatatt ttagtccgct catatgacag
     5881 ctcgcttgat gccggaaaca catactataa agtaacgtat gatgtaaagg gagaaaaatc
     5941 tgaggaaaat gtaaaggaaa atacaagtcc gttaaatatt ccggacttaa caaactatgg
     6001 atatatattt aaggggtggc aagtaaatga tgacactgaa aatctgattg atgatttaaa
     6061 ggaatataaa attacagccg atacaaaatt tacggcaatt tatgaaaaag atacggaata
     6121 tattgagcca ataatacaat tacgcttaca ggtcagaacg gaacgattat tacagccgat
     6181 acgatagaca gccgaataga cgattttaac attgagtggg atatagacgg ttttaaaact
     6241 gtaaacgata ctgataaata ttgcgatagc tatggcgagt ttgccgagca tacgaacact
     6301 gcgacagagg ttttgtttat gctcagacaa tgcgatttta atttttatgg taagctaaat
     6361 gccacagtga catataacgg cgaaacaatc gaagcgtcaa tgtatgttgc cgcaacaggt
     6421 gataagagca tggcagataa ccaaatcttg cctgaagcag gctacccatc cgactttgac
     6481 gaatatcccg acagtcttgt tggatatact acattaaagg acacttacgg cggtaacgat
     6541 attatgg
//
LOCUS       DAWACS010000031         6538 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1333_length_6538_cov_2.044424, whole genome shotgun sequence.
ACCESSION   DAWACS010000031 DAWACS010000000
VERSION     DAWACS010000031.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6538)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6538)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6538
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1333_length_6538_cov_2.044424"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5230..5239
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1721 a         1533 c         1045 g         2229 t
ORIGIN      
        1 gaatgtccat gccataatcg gcacactaag caatatttta gggtatttct tcaaacagta
       61 aaaatagaag tcctttgtgc tgtcactgtc ataaattgtt tcatcaaaat cgtaaatatt
      121 caagccatat atccccctat ttcaataata caaccgccgt tgcggaaatg ccctcacctc
      181 tgccctcaaa gccgagtttt tcggttgttg tcgccttgat attgacatca tcaataccga
      241 cattcaaatc atctgcaaca ttttttctca tttgctcaat atacggtaac atcttcggtc
      301 tttgtgccac tatcgtaaca tctgcattac ccacggaata tcccttttca gccactttct
      361 ttacgacttc tctcaaaagc atacggctgt ccgcaccttt atacttttcg tctgtgtcgg
      421 gaaaatgttt tcctatatct ccgagtgccg ccgcaccgag aattgcgtct gcaagtgcgt
      481 gaagtgccac gtctgcgtca ctatgtccca aaagtccctt ttcatacggt attttaacac
      541 cgcaaattat acaatctctg tcctctacaa gcttatgaac gtcatatccc tgtcctattc
      601 tcattccgta atacctcgct ttctgagtat tctttcaccg attatcatat cttcgggagt
      661 tgtgagcttt atattatcat aacttccgtc acttattttt atctttacac cgtaatgttc
      721 ggcaatcata caatcgtcag tagcgacaaa attttcgtca agtgcttttt ggtgcatatc
      781 aagtatctta tccagataaa acacctgcgg agtttgaatc ataaatgttt tttctctgtc
      841 aacagttcct gctataaaac cgtcactgtc cgcacttttc agcgtgtcct tgcatttaac
      901 accgaccgcc gccgcaccga attttataca atccgcaaca gaattattaa tttcatcatc
      961 tgtcacaagc gctctagcgc cgtcgtgtat aagcacaata tcaccttcgg cttttttcag
     1021 tccgttcata acagactcct gacgtgtcgc accgccttct gttatatatg ataccttttt
     1081 tatatcgtat ttgtcaacaa gtatttgaca ttcttctata tcatttttgc ctgtgacaac
     1141 tataatatca tcaattttat catttttttc aaatgccgaa attgtataat aaagcacttc
     1201 acgtcccaat attttaagaa aaaccttatt tttatcggca cccattcttg tgcctttgcc
     1261 tcctgcaact ataaccgctg ttattttcat aatatatcct ttcaaaataa aagcagagcc
     1321 ataaaacata gctctgcact tttaatcaca tatcaaacca acgcgtcaac agtatcgttt
     1381 aaaatgcttt caatatcact aacatcggca acttccgaca ggacaagttc gcttacaaca
     1441 atctgttttg cactggtaag agtctttctt tcacttgttg aaagaccttt tattttttca
     1501 cggtacatca gttctttcaa taccaaaatc acttgataaa tatctcccga cttaatctta
     1561 ataagattat ctctctgacg tttattccaa ttcgcgtcat cttcaagctc cgcgtcacga
     1621 aaatattgaa gtgccttttt tgcctcctgc ttttctataa caggacgaat accgatttct
     1681 tcactgtttt ctatcggtac atttgttacc atattgccta tggcaaactt tagaacatag
     1741 tattgacgtt ttttgccgac aatctctatt tctttgattt cttctattgt acctgcaccg
     1801 tgcataggat gtacaacttt atcgccgaca ctatacatct ttacttaccg cctttctcat
     1861 taaccactat cttaacaaca taattataac acaaaacgcc ccaaatgtca aattttttga
     1921 ttatatcata gttaccaaaa aaaatcaacc ccttttgtgt aattttttta ataaattgtc
     1981 aaaataaaaa ggataccact ctaaagtaat atccttttcg tttttattct ttattttgac
     2041 ctgtgttgtc gggaattatg ctttccaagc gacccgccgc agtaatcgca agatttttaa
     2101 gcaaagtctt ttgattatcg tcactgcccg aaccggctgt aatctcaatt atacagcctc
     2161 tgtcgtacac atggatagtc ggaaacgcta tatacgattc ctgtcctata ccctcaacca
     2221 attcagcaga agaacgtttt gacttttcct gttcgtactg gtcgaaaagc atttgataat
     2281 caatagtatc tgtgaactgt gtaaccttaa ccgttacagt atcattttga cctataggct
     2341 cacttctgta aagcactgtc gcaacattac cgttacggct tgtacctgat tcctcaataa
     2401 caggtgtata tccggcattt agtgcaacat catcagttgt gataagtgtc gccgggtcga
     2461 tttcaggtat cggtgtaggt gtcggctttg caaaaagtcc gccgccaccg ccgcaagcac
     2521 acaaacctat tatcatggcc aagcttgcta acacaagcaa taactttttc ataattttac
     2581 ctccaatgat tatttcattg caatggcatc ttttacattc ttaacgatat tttcaggagt
     2641 taaaccgtat tccttaaata attcagccgg tttacccgat ttaccgaatc tgtcagtacc
     2701 gataatctta acaggtacag gagcatttgc acataccact tccgtaacgg cacttccaag
     2761 accgcccata atgtaatgct cttcagctgt tacgatagca cctgtttcct tagcagcctt
     2821 tgttattatt tcttcgtcta taggcttgat tgtatggata ttgattactc ttgcggaaat
     2881 tccttcttct tcaagcattt ttgccgcttc aagtgccata cctaccataa gaccggttgc
     2941 aataattgtt gcgtccttac catcctttag ttgaacgccc ttgccaagtt caaacttgta
     3001 gtcctcactg tttacgtcct caacggcaag acgaccgaat ctcatatata caggaccgtc
     3061 gtggtcaata gccgccttaa cagcttgcat agcctcaata tcatcggcag ggtttataac
     3121 aaccatatta ggaattgaac gcattaaagc gatgtcctca agacattgat gtgacgcacc
     3181 gtcctcaccg acagaaatac ctgcgtgagt agcaccgatt ttaacattaa gctttgtata
     3241 gccgattgag tttctaacct gttcaaaggc tctgccggcg gcaaacattg caaatgttga
     3301 cgcaaaagcg atttttccgc ttgcggcaag tcctgctgcc acgcacatca tattactttc
     3361 tgcaataccc atatttataa atctttcagg atatgctttc ttgaacattt ctgtttttgt
     3421 tgattttgaa aggtcagcgt caagcacaac tatgttaggg ttttcaccgt actttacaag
     3481 cgccgctccg tatgattctc ttgtagcttt ctttgccatt acagactcgc ctccaatcct
     3541 ttgattattt catcaagctc tgcgattgct tgattatact gttcttcatt aggtgcagaa
     3601 ccgtgccagc ctgcgttatt ttccataaat gaaacattct tgcctttaac agacttcatt
     3661 ataatagcgg taggctgtcc ctttgtagcc tttgcctcgt caacagcagc cataagttct
     3721 tcaaaattat gagcacttac ctttataacg tgccaaccga atgcctcaaa tttcttatcg
     3781 ataggattag gattcattac cttagaaatg tcaccgtcta tctgtaagcc gttgttatca
     3841 acgaaaattg taaagttgtc aagtttgtaa tgagcggcaa acatagcttg ctcccaaacc
     3901 tgtccttcct caagctcacc gtcaccaaga acagaatata ctctgtaatc cttgttgtca
     3961 agctttgcgg caagtgccat accgcctgcg gctgacacac cttgtcccaa tgaacctgtt
     4021 gacatatcaa cgccgggtac tttgttcata tcaggatgtc cttgaagata gctgtttaca
     4081 tttctgaatg tcttaatatc ctcttttggt ataaatcctt tttctgcaag tacaccgtat
     4141 agtgcaggtg ctgtgtgacc ctttgaaaga acaaatctgt ctctgtcctc cattttagga
     4201 tttttcggat ctacgttcat tacctcaaag taaagcaatg tcaaaacatc tgctacagat
     4261 aatgaacctc ccggatgtcc cgatgccgca ctgtatacgg ctgtaagagc gtgcttacga
     4321 acattgtttg caatgattga cagctctgtg actcttttct tatccatgtg tcgtatttcc
     4381 tcccttaaat taattattat ctaacgcatt ttcatacgcg atattcataa gtgtgtcaag
     4441 tctttccgtt tctcccgaaa gatacagaaa tcctataagt gcctcaaatc ccgttgctct
     4501 gcggtattcc gtcatatttg cgtttttcgg cactgtattc gattttgcgt ttcttcctcg
     4561 cttgtatacc gccgtttcat catctgtcat atgttcaagc attgcgtcta tactgtttga
     4621 ctgtgcgtgt gctttgacat actgaacagt ctttttatgc agtttgtttg caggaagatt
     4681 ttcgtgttct tctataattc gtgtacgaac aaaaagttca tacactccgt cacctatata
     4741 ggcaagcgcc aaaggcgaaa actgtgacgg ctttttagtt gtaagcatag ttacctcccc
     4801 aagtttaagg atttctcagc aagtgctgtg ggtaaattcg ttcacagtgc tgttttgaaa
     4861 ctcgggtgct gtatactgat accgccccgg ttgcatggcg agcagcagac cttttgtctg
     4921 cgaccagccc gagtaaaaaa tagtgctgtg ggctaatttt tattctgaga tgtagttcca
     4981 ctttacaccc atcggtgtat cctttaactc aatattcata gctttcagct tatcacgaat
     5041 tgcgtcagct tcagcccagt tcttctcact tcttgccttg ttacgctttt cgattaattc
     5101 ttcaacctct gcgtctatcg acttttcctg tgtcttttgg aataaaccga gtacaccgcc
     5161 aagctcgtgt ataaggtcaa gcgtcttttc aacaaccgct ttcgcattct tattttcgtt
     5221 tgacaatgcn nnnnnnnnnc tgtctgtaag ttctctgtcc tcactatttt gtagtaaaaa
     5281 ttcaagattg tctatgcaag tgtaaactct ttcaacagca gattttgcct gttccattaa
     5341 ttcattatca aaattaatag gatttctgta atgagccgaa agcataaaga aacgtaccac
     5401 ttcaccgtca tactgtttaa gtatgtcacg aacagtaaag aagtttccga gtgacttact
     5461 cattttcctg ttatttatat taatatatcc gttgtgcatc caataatgtg caaaaggctt
     5521 tccatttgca cattcgctct gtgcaatttc gttttcgtga tgtgggaaaa tcaagtcttg
     5581 accgcccgaa tgaatgtcta tcgtttcgcc aagatacttg ttcaccattg cactacactc
     5641 gatgtgccaa ccgggtctgc ccattcccca tgggctttcc caagcaggtt cgccctcttt
     5701 ttgctttttc caaagtgcaa agtccataac gtccttttta tgttcgttca catcaattct
     5761 tgcacctgcc tccaaatctt ccaatggctg ttttgaaagc ttgccgtatt cgttaaattt
     5821 ctttgtagag aaatacacgt ttccgtcaac atcgtaagca tagccgttat caacaagttt
     5881 ctttacaaca ttgataatcg catcaatatt ttccgttgct ttcgggtgaa ttgtagcttt
     5941 gtgaataccg atagcctgtg cgtccttaaa atattccgca ataaatcttt caccaagctc
     6001 ttttaccgtt ataccttctt catttgcacg tttaatcatc ttatcgtcta tatccgtaaa
     6061 attctgtacg aatgttacct tatatccttg atattcaaga taacggcgca ttgtatcaaa
     6121 aacaataaac ggtcttgcat ttcctatatg aaaatagtca tatacagtag gaccgcatga
     6181 atacatttta acttcacctt tatttatagg gacaaactcc tgtttttgtc ttgttaatgt
     6241 gttataaatt ttcataagca ctacttcctt tttccattct aactttaatt atatcgcaaa
     6301 ataaaagaaa atacaacctc tatttttaaa aaatagaagt tgtatttatg tttttatatg
     6361 ctcggaataa ataatctgtt tcctatttca agtttgtcgg atttttccat attgttaaac
     6421 ctcaaaattt cgctttgcgg tactgcatat cgttttgcaa tctcccaaag attatcccct
     6481 ttttgaacaa agtaaatcac tataccgttt ttgtcaccgt tttcaagcgg ttctgtca
//
LOCUS       DAWACS010000032         6326 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1385_length_6326_cov_4.487163, whole genome shotgun sequence.
ACCESSION   DAWACS010000032 DAWACS010000000
VERSION     DAWACS010000032.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6326)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6326)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6326
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1385_length_6326_cov_4.487163"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1886 a         1331 c         1101 g         2008 t
ORIGIN      
        1 ctgcttcttt cccccttttc gctcttagtc ccgacaggac agggggtgca gttgcatttt
       61 tgaataaaaa aatacaccca ttttaaaata aaaatgagtg catgagaaat aacagtagaa
      121 taaatatgtc ttgtcagttc atattcaaag tcggactccc acagtggagg agctatactt
      181 tttttaatga atgtaaccta tgtatgctgt acaacaacaa agaaatcccc atagcggcaa
      241 tcctcctaaa tgccgctatt tatattctgc gtcgtcgtta gttcagtaat agacaaaaag
      301 aagcggcggc tctaaaaaat caaagccacc gcttcaaaag cgcgtgaaaa tctatctgcg
      361 ttacgacggc gttttttctt ttgccgtcgt ccggcagatg tttatagaat tttgatgaat
      421 cgctattaca attcattgat tgtttcagta attgccatat tgcgtatgcg ctttgccggg
      481 gtatgaattg atataataca cgaaaaaata accagtataa acaccatagc aatagtggta
      541 aaaggaatat tccatgagcc accaaaatga gtaataacaa tcttcgtata aatcagatag
      601 tggagcagca atccaagtat aattccgaca gtagtaccac agatagcgta tgtggctgct
      661 tccgcagtta tcatttttgt aacctgcctg ctttccatgc caacagcacg catagcccca
      721 tattgtttaa ttcttgctga tacccccatt gaaacgctgt tcataatatt caatatggtt
      781 attagtgaaa tgatagcaag aaaaccgtat gccgcaattc ggaacaccca ataggaacca
      841 tttacttcat cattttgtgc tctgctgtcc gcaactacat ttccttctga tagctcataa
      901 attttattta ctgccgtatc agatacatct ttttccaata caacacttaa catagcatag
      961 ccttgttcgc ccattagacg tgtgtaagtt tcctccgaac ataccacaac tgccgaaccg
     1021 ctaacactcc ccacaccctc agacacaacg cacgcaacct caagttcttc tgtacctatt
     1081 tttattttat ctcccacatt tagtcctgtt tggtcactgt aaaccgccat tacatattga
     1141 gaatttccgt acacttttga tatatttccg cttacaacag atttcttaaa gctgtccatc
     1201 atagcgtccc cgtgagaaaa taaatcaatc gttgatacat ttccgttaat ttctgtcgga
     1261 tattctgtgc tatacatcat gccaaaaaca gattctactc cagatatatc agaaattttt
     1321 tctgatatgc ttttgtctat ggaattagat tcatcttgac tgctaatcgt aatgtcagga
     1381 gtaaaatcac tcactgatgg aagcaattta tgaacaatat caagacacgc cgaaaaagtc
     1441 aaaaaaagca tgattgtaag tgcaaaggag cctgtcataa ggattaggtt tttctttgtt
     1501 ctgattgcat ggtgaacgcc caaagaagtt tctactttga aaaaccttgt atttgccgca
     1561 tgaacaatat tttttgatgg ttctgcattg ccggatattg ctgctgcggg cgaaactttt
     1621 gctgcctgtt tagccggaga ttgagctgcg ataaatacgg taataatacc tatggcagct
     1681 ccgcagagaa taccacttac acttactgaa aatagcggca tatctaccca ttcgcctttt
     1741 acgataaatt ttaatatcaa acatactacc cagcaagtta ctgttcctaa cgcgcagcca
     1801 atcggaattg ctgttttaca ccaattcagt gcttcaagcc ttacaaaacg cacaatttgt
     1861 tgcttactgg caccgataca acgcatcatt ccgaaaaatt ttgtcctttg cgcaacattg
     1921 ctattcatgc agcttgcaat cataaagatt cccgcaatta gtataatgat aaagcaagct
     1981 attgccaaag gataaagtgc atttacgctt tcgttgttgc ttgctcctaa caggctcaaa
     2041 accgccgtat tttcgctaat attctcagca gtgagggaat attgctcttt tatatcagta
     2101 atcgtttttt tcaaaccatt ctcattttca aaccgaatat aatattgaga tttaggttct
     2161 attccatttt gatttcgtac ttcatcaaat gtggctctgc acatatatat acagacaccc
     2221 tcaataatgt cattaaactc ctcatcatct tcataaaatc cagatattgt atagctaaaa
     2281 ttgccagcag gcgtatttag aattatatcg tcgcctattt gaatatcaaa attgtttttt
     2341 gcgtccgcac taattgcaat ttcgttttct gtttgtgggt atgaaccctc caacggataa
     2401 ttcataatat ctgtaacata ggcttcttca ataccataca gcacaacatt ttttccgttg
     2461 atataataat tgcaatctac atcagtattg atttcatcat gccatgagga atatgccacc
     2521 tcagaattat ctataatctg ttcaacttta gcttctggca tattttgaag aacaatgtgc
     2581 caatccccat gattactccg cattgctgtt atttctccat ctgtccacat ctctgccaaa
     2641 gaaaagattg cagttacaag gaatacggca agaacaatac aaatgcgcgt catgcggctt
     2701 tgccgtttgt gtacttttgc agaaatcggg ataagactta aatagctttt cattcgcggc
     2761 acctccctaa atcggtaagt acaccgtctg atacctgcaa tattcggtct gcgctctgtg
     2821 caatgctcct gttatgtgta atcataataa tcgtttgctc atacttcctt gacgcttctt
     2881 tcaacaaaga aatgacttcg ctactgtttt gcgtgtcaag gttgcccgtt ggttcgtcag
     2941 caagaattag tgacggacgc gtaataagag cacgcccaat agctacccgc tgttgctgtc
     3001 cgccggataa ctggctcggc aaatggttgc gacgttcttt cagattaaga acggtaagca
     3061 gttcttccag atattttttg tcaggctttt gatagtcaag cagaactgga aaaataatat
     3121 tttgttcaac ggttagctcc ggcacaagat tgaacgcttg aaaaataaat ccaatatttc
     3181 tacgtcggaa aacagtaagt ttgctgtcgt tcatagaaaa tatatccttg ccaccaatct
     3241 tgactttacc agacgtaggt gtgtcaagtg cacctatcat attaagtagt gtacttttgc
     3301 cggagccgga ttctccgacg atagccacat actcaccttt tggaacagaa aaactaactt
     3361 ttttcaaagc gtgtacggca gtttctccag ttccgtaagt tttacagata ttatcaactt
     3421 ctaacaaatt catgtgtaaa cacctctttc tgttttgata atctcaatgt atcaaagaaa
     3481 tcctacatca atattacaat cagcctacaa ttttgtagga ttaagaaagt ttattgtgaa
     3541 agaagtgcca gtgctcaatg tactatcaac ttcaatcgtt ccgttatgag cttctataat
     3601 cgcctttgca agtggtaaac caagtccgat accctgtaca tctttagaga agcgactccg
     3661 ataaaaacgc ttaaaaatat ggtgcaaatc ctcagggtgt atgccgctgc cattatcctt
     3721 tactgtaatt tgaacaatag aagcgaacgc ccgccactct atacggataa taccgccttt
     3781 ttctgtgtgg tcaagggcgt tctttacaag gttgccgata gcttctatta tccagttacg
     3841 gtcacataaa agtgaaattt cttcattgcc cgataagtgg atttcttttc cctcctgttc
     3901 agcacgaaac aggaaatgct tttttatatt ctctgcaagc tcggacacat tttctaatga
     3961 tttttctaac tcaatcgttc ccgcgtccag ttttgtaatt ttcaaaagac tatgtaccag
     4021 tgtttcaata cggtcaagtt cctgctcgga aaactggcta aattcttgta tggtcggtaa
     4081 ttcctttgcc tcgttctgta taatgccatt ataaatatta agggcagcaa gtggtgtttt
     4141 taactggtgc gaaatatccg atatggtatt gcgtaaaaat ttttttgcac ttttctcatt
     4201 ttcggcatga gcgttcaaga ttgcaaccaa agaattaact tcatggaaca gcctgtataa
     4261 ttcgccctcg tcattacact caatcgtaac gtctttgttg ccggaaatat attctgtaat
     4321 ctgtgatatg gcattttcca tgattctatg atgttcccta aaatatccat agcaaagcaa
     4381 caatattata atccccatac ataggctgca aattataata taataggttg cattttttat
     4441 ttcaaagttt gctaataata tggaaattaa cgtgaaacct atgatacaag ataaaatatt
     4501 gacaaaaagg catttgattt ttttatttgc aaatattttc attatatttt cgcttcctcc
     4561 gtattccatt tatagcccat gccgcgaaca gtaacgattc tttttggttc tcctggatta
     4621 tcctcaattt ttgtacgaag tctacggata taaacggtaa gagaattatt atctatataa
     4681 ttttcgttgc aatcccatag atttcctaaa atctgttccg aggaaagtat ctggtcagga
     4741 ttttccatga acaggcataa caatttatat tcacttgcgg ttaaatcaag cagtttcccc
     4801 tgtttataga cctcaccttt tagtagcctt atacttatat cgttggagct taattctgta
     4861 acggaagtat tgaaattctc actcctacgg agcaatgcgt taatccttga cataaatatt
     4921 gctaatttga aaggctttgt aatataatcg tcgccaccaa tatcaagtcc cataataata
     4981 tctgtttctt cgtcagcagc agtaagaaat atgataggta cttttgaagt cagacgtatc
     5041 tttttgcaaa aatcaaagcc ggaaccgtcg ggaagcgata cgtccaaaat aactaaatca
     5101 tatttatcat ttatccataa tgtatcagct tccagtgtgg tgcgggcaac atctatttca
     5161 tacccctctt tttttacggc aaaagtcagt ccgttaatta agctcaaatc atcttccaat
     5221 aaaaatagcc gtttcatttg ttatcacatt cctttcctat ttttgttctc ccatcaatcg
     5281 gtttttctgc gtcttttggt atcagccaca tatacccaag ttttgaaaca ccgggtatgc
     5341 ggttctctct gcatagtatc tgtacacgcc gttcagaaat cccccatttc ttcgctgctt
     5401 cggggcaaga catatattcc ataacaccca agtcctttct acaaatcata tcagtataat
     5461 tatagtcggg catacgaata atagcaagtg caagtatatg aattattatg gctataaata
     5521 ggaactgtat agacgtattc agtatgaaag aggaacagta gaatgtatat gggtgaataa
     5581 atatggcaaa aagaccaaca gctaaatatg actttaaggc tttcggacaa gctatcaaag
     5641 cagcgagaac cgaacgaaaa gagagccgta aaaaagtaag tgatgaaatg tatatctcac
     5701 cacgttatct tgctaatatt gagaataaag gacaacagcc gagtttacaa attttttatg
     5761 agcttgtaac acgctatgat atatccgtag accaattctt tttcccagat aaatcagcgg
     5821 aaaaagatac acaacgtagg cagcttgata cactgcttga tagtatgagt gagaaaggaa
     5881 tacgggtagt caccgccaca gcaaaagaga tagccgaagt cgaaacagag gacaggggaa
     5941 tgtccagaac agaattttaa cgagaagaca cattgtaatc tttcaggaaa tacaatgtgt
     6001 cttctgcttt atataactct atttcacaat cttccagttt tctccgttct tttccagtac
     6061 aaggtcaaac tgtgatacct gtgttgtcat ggtctggtta tccagatatt tcaccgcaag
     6121 ggaagccgtc acctgatttc catttcggtt gtagactgga ttcaccagtt cggagaaaac
     6181 atattctttc cccacaggtt tcagcacgtc ctcattcaca taataggtca gttcctttgc
     6241 ggttgctgta gggtacagtt tgaaaaaggt ggtgagaaat tcgttgattt cctctgtggt
     6301 gatggaatcc accgtaccgt cacttt
//
LOCUS       DAWACS010000033         6310 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1390_length_6310_cov_2.818225, whole genome shotgun sequence.
ACCESSION   DAWACS010000033 DAWACS010000000
VERSION     DAWACS010000033.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6310)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6310)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6310
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1390_length_6310_cov_2.818225"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    251..260
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2095 a         1023 c         1478 g         1704 t
ORIGIN      
        1 aaattatgag cgaatcaatt aaaatttgca ttcaaaaata tgtgaaaaga tagccaccac
       61 tccggcacta tgtgccacct ctcctcaagg agagactttc ggctcccata gaggggagct
      121 gtcggcgtag ccgactgagg ggtggcaaat atgaaaaagc ataaatcgtc tgtttgcgta
      181 tacaataaat ccgtaggggc gatcattggt cgccctagcg aatataaaat taaaaccacc
      241 actccggcac nnnnnnnnnn aaatatcggt atgggaaaaa tcataccgat attttttatt
      301 tataatatta aaattttttg aatttttcac caaaagggta tactaaaaac gatttttttg
      361 ttatactgaa tatatagaaa caaaaagcga ggtaaatatg gagtataacg agcaaaaaca
      421 ggaacgtgta attttagcgg gtgtacatcg cggtatgcgt gacgcacttt gggatacaac
      481 tgaagaatca atacatgagc ttggcgaatt ggtgaaaacg gcaggcggta ttgttgtggg
      541 tgaaatgatt caaaacaagg ctgatttgga aagtgcaacg tatatgggcg agggcaaact
      601 tgatgaactc aaaattgcca tagaaacgct taatgccgat atggttgtgt ttgatgatga
      661 gctttcgcct gtacaaatga gaaatataag tgattttctt gaaataaaag tgcttgatcg
      721 ttctatgctt atacttgata ttttcgcaat gcgtgccaaa agcggcgagg gtaaacttca
      781 agttgaactt gcacagctta aataccgctt gccgcgactt cgcggtttcg gtgtggaaat
      841 gagccgtaca ggggcgggaa taggcacaag aggtccgggt gaaacgcgtc ttgaaagtga
      901 cagacgtcat ataagacgaa gaatttcggc acttgaagaa gaaatcaagg aactgaaaaa
      961 acatcgcgga cttattcggg acagacgcaa gaaagacggt gttataactg cggcacttgt
     1021 gggttacacg aatgcaggca aatcaacgct tttaaacacg cttacagacg cacaggtgtt
     1081 tgcggaagat aaacttttcg caacgcttga ccctacatca agagcgataa cacttgacga
     1141 taacagaaaa atattacttg tcgatactgt tggttttata agaaaacttc cgcaccatct
     1201 tattgaggct tttaaatcaa cacttgaaga agcggttgtg gcagatgtgc ttttacacgt
     1261 tattgatgcg tcgggggaag aaatggacaa tcaaatcaca gtcgtggaac aagttttgtc
     1321 tgatatcggt gcggtcggta agccggttgt ggcggtgttt aataagtgcg acagacttga
     1381 agattatccg ataacgaatt taaagagtga caagtgcgta tatataagtg ccaagcacag
     1441 aaccaatatt gataaattga ttgaagcgat agccgacacc gcacctggta aaaagcagaa
     1501 agttaaggct tgcataccgt acagtgcagg ttcgcttgtg aatgaacttc atgaaaatca
     1561 aaaagtaata tcagaagaat acggcgaaaa cggcactgta atggagctta tggtagacgc
     1621 tcaaatgtat gataaaataa gggagtatat tttgtaaaat gtcaaaaaaa gacgtataca
     1681 aaacggtaaa aaagcagtcg cagtcactgc ttatagagaa aaaatcaaaa tttattgcaa
     1741 atgtaaaacc tgttgacaat gaagatgacg cacttgcgtt tttgaatgaa atgcgttcaa
     1801 agtattcaga tgcaacgcat aatgtttacg catatgtgat tgacgaaaat aatatttttc
     1861 gttacagtga tgacggcgaa ccgtcgggga cggcaggaat gcccgttctt gacgcaatcc
     1921 gtaaatccgg gatagttgat gtggttgtgg tggttacgcg ttatttcggc ggtacacttc
     1981 tcggaacggg cggacttgta catacttacg gagcatcggc aaaacagggc ctgattgagt
     2041 cgaaaatagt tataagaaag ctttgcgata tagtttctgt aaaagttgat tattctctgc
     2101 tcggaaaaat gcaacatgag attatgacta aaggatattt gcttgaggac actatttacg
     2161 aagatgaggt tacttttatc atatcgtgtc cgattgatac gaccgataaa gttattgcgg
     2221 aaataatcaa tatgacgaat gcaggagcgg tttgtgaaaa gattgataaa aaatatgttg
     2281 atgttaagga ggaagaggga tgaagaaagt aattaaagcg atatgctgta tattggctgc
     2341 cgttatactt gtcggtggcg gatatgtggc aggtcagaga gcaaaacaaa gcagtatcgt
     2401 atcggcactg cctaaaaata ttctcgatat gccgaacgag agcaatgagc cttcggaaag
     2461 tgctgaacct gagagtaagc cgtcggaaga tgtgcctgaa ccgaaagagg atgtaagaat
     2521 aataaataaa tcgaatgagt cagcagttga ttcaacgtgg agtacagtta aatcatcgag
     2581 caacacaacc gataacggtg cacttacatt atacacttcc gcacaaaaag acggtgatga
     2641 atttatttgg gacgacagtc aaaagtgggt gcttgaagta tcggacggta acggcggata
     2701 ttatacattg tttgaccaaa atgtaagtaa cggaagtgtt tattacgatg ttgccgaaac
     2761 cgacaacggt acgaaaaata taaatgtata tgttttcacc ggtgcgggaa caacaataaa
     2821 gcaatataca cacaccgaaa cgggattttc cgaaaagagc gtgtatgaat ccggtgctgt
     2881 aaacagagtg tttacaacaa tcccgtcata taaataacac agttcataga ggaggaaata
     2941 caaatggaaa tcgaattgac aaaggaaaac tatgatggtg aagttataaa gtcaaatatg
     3001 cctgtgcttg ttgacttttg ggcttcttgg tgcggacctt gcaaaatggt tgcgcctata
     3061 atctctcaaa ttgcagagga atacaacggt aaagtcaagg tgtgcaaggt taatgttgac
     3121 gatcaaggcg agcttgccgc tcaaaatgct attgtttcaa taccgactgt aattttattt
     3181 atagacggca aacctgttaa gaagattgtc ggcgcacatt ctcttgacga ttacgaggac
     3241 gcaatagatg aatttatatg attaaaactg attaattatt cagtacagtg cataaatatt
     3301 catactcgat gaatatttat gcactgtttt tatatgattt aaactttttt gtcaaaaaga
     3361 cttgcatttt tattcaaaaa ggagtataat cataaacgta tttgaaattt tggagggtta
     3421 gtaatgcttg atgaagttat acaattagat aaaaaatatt atttgaatac tttcggtgac
     3481 agacttcctg tatgctttga aaagggcgag ggaatgaaac tgtttgacga taagggtaat
     3541 acatattacg attttcttgg cggaattgcc gtaaatgcgt tgggtcacgg acacaaagat
     3601 tttataaacg cacttaaaaa tcaacttgat aaagtagtgc atacatcaag tctttattat
     3661 atagaaaatc aagcaaagct tgcacaaaag cttgttgaaa atacttgtgc ggaaaaagcg
     3721 tttttcgcaa acagcggtgc agaggccaat gagggtgcat tcaaacttgc aaagatttat
     3781 tattataaaa aaggtttgga taagtatgag ataatcacgc ttgataagtc attccacggc
     3841 agaactcttg caaccgttgc ggctacaggc caggaaaaat atcaaaagcc gtacagaccg
     3901 ttgacaccgg gtttcaagca ggttgagcct aataatttta aagcgattga ggacgcggtt
     3961 actgataaaa ctgcggcaat tatgattgaa cttattcaag gtgaaagcgg agttcatccg
     4021 atggataagg aatacgttga aaagcttaga aagctatgcg acgagaaaga tattattctt
     4081 atttttgacg aagtacagac cggtatgggc agaacaggtt atctttttgc acatcaaatg
     4141 tacggcgttg aaccggatat tttcacttcg gcaaaggcac ttggcggcgg tattccgatt
     4201 ggtgcggtat gtgcgggcaa aaaggttgcg tctgcatttg aaccgggtga ccacggaaca
     4261 acattcggcg gtaatccgtt cgcaacggcg gcaggattgg ctgtgtttga tatttttgaa
     4321 aaggaacatc ttgttgaaaa tgcaggtaag atgggtaagt attttaaaga aaaacttaca
     4381 gaacttcaaa ataaatattc ggataaaatt acagatgtca gaaatgcagg tcttttgatt
     4441 ggtattcagt tggaggacag catagctaag actgtattta ataagttgtt tgaaaataaa
     4501 atgcttacaa gcctttgcgg cggcaacaca atcagaattg caccgccgtt aattataaaa
     4561 gaaacagaca tcgacttgtt tattaataca ttaagcagta tattggagga attataaaat
     4621 gaaagattta atcagtttac acgatttaac ttcagaagaa gtaaaaggac ttttggagct
     4681 tggtttaaaa ttaaaaaagg aacagaaaag cggaattgaa catcatattt taaagggtaa
     4741 aacacttggt atgatattca caaaatcatc aacaagaaca agagtatcat ttgaagtggg
     4801 tatgactcaa cttggcggtt atccgttgtt cttatcgtca aacgacattc agcttggacg
     4861 cggcgaatca atttacgata ctgcaaaggt tttggaaaga atgcttgacg gtattatgat
     4921 tcgtacttac gcacatcaag atgttttgga ccttgcagaa tatgcaaata ttcctgtaat
     4981 caacgcactt acagacttgc ttcacccatg tcaagtgctt gcagacctta tgacaacata
     5041 tgagcataag ggtaagcttg agggcttgaa acttgcatat atcggtgacg gtaataatat
     5101 ggctcattcg cttatgtacg gctgtgcaaa ggcaggtatg gactgtgcaa tcgcaacacc
     5161 tgaaaattat cagtgtgatg cggaagtggt tgcaaacgca aaggacgact tcaagaagtc
     5221 ggggaaagaa ttgattttga caacagaccc ggttgaggca attaaaaatg cagatgtagt
     5281 ttatacagat acatgggtat caatgggaca agaggctgaa aaggcagaaa gacaaaagat
     5341 atttatgcca tatcaagtaa acggtgaatt gttcaagaat gccgcagatg acgctgtatt
     5401 tatgcactgc ttgcctgcat atcgcggttt tgaagttaca gaagaagtta tagacggacc
     5461 tcaatctgtt atttttgacg aggctgaaaa cagacttcac gctcaaaaag cagttatggc
     5521 aacattgatg ggataatcgg aggttatagt aatggaaagt gattatcagt cattatttca
     5581 agtgcgtttg gctaattatg atgatatacc cgcaattatg aatatcacgc gtgaggcatt
     5641 tgttaaatat cagcagatgt caggcgttga aaatcttgcg gcacttgaag aatcgtataa
     5701 tgatgtgaaa aaagatatag atacaaaagt tgtattgctt gcattgtcgg acggcgaacc
     5761 tgtcggcagt gtgagagtag aggtatttcc ggataaaacc gcatatttgt cgcgttttgc
     5821 ggtaaaggtt acaagtcaaa ataacggtat cggtaaatca ataatgaatc ttgttgataa
     5881 aattatggtt aaaaagggtg taactcaaat tcagttgcat acaggctcaa agattacatc
     5941 gcttattcgt ttttattacg gcagaggatt ttatattgat tcgacagata agtcgcgagg
     6001 ttatgttcgt gcattgctga ttaaggaata tcaaaagaaa gattgttagg taaaagggac
     6061 actaaaattt agtgtccctt ttgatatgca gtaaatataa ataaaccttg tgggcggccg
     6121 atgaccgccc ctacaaatag gtagtcgata attattgaaa ttcgtagggg cgaccgttgg
     6181 ttgtccaagt agtatgggac ggcgcacacg acctccggta aaagccacac ctcagtcaac
     6241 tgtgttgaca gctcccctcg atgggagccg aaaagcctct ccttgaggag aggtgtcacg
     6301 aagtgacgga
//
LOCUS       DAWACS010000034         6080 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1447_length_6080_cov_1.897593, whole genome shotgun sequence.
ACCESSION   DAWACS010000034 DAWACS010000000
VERSION     DAWACS010000034.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6080)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6080
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1447_length_6080_cov_1.897593"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2118..2127
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4941..4950
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1996 a         1048 c         1429 g         1587 t
ORIGIN      
        1 ctggtatata tgctatcatc gctttaatgt tccgagatac ggaactgtca cgtccaaaaa
       61 cagtgaagcg ggtaaccaca gagaaatttg tatagaaaaa atggagttcg atgaaaacgg
      121 caatattaaa cctgtaacag ctacgttaaa aggaataaca gagcctgtta cgattgaaag
      181 gtaaatactg cttgattgct gaatgaaaca gggaggagaa tcatatatga aaagaatatt
      241 atcaatccta atagtgctga cgattgtact tacatcgttt agcgtaacga gctttgcggc
      301 agaggaaaaa gcagcattgc ccaccataac tgttgatatg aaaaatgagc agcacaacat
      361 aattcatggt gcggcaggtt tcctttacgg aataagcaat gagggagtac cggatgtaaa
      421 cacgttaact cctctaaaac caaaagttct tgcaactaaa ggtgcattgg gtacggagca
      481 tccttacggt gatgcacttg atgttgccga ggagtttttt gaagccggcg gcgaacaggt
      541 gcagatgtac aactccaatt attacggtgt attcggagtt acggcagatg cttatgatta
      601 cgcagatgta ctcaaaaata taatatgtcc ggaagtagta gcatggaagg aaaaaatgcg
      661 cgaccgtttc cctgacatag ataaaatgct tcaatatatt ccgattaacg aaagcacacc
      721 tataaaggtt agcggcagca agaattataa tgaagcgtgg aaaatttatt acaaggcaat
      781 aaaagaagca gacccgaacg ccactgttgt agggcctaat catgcatatg atccaagcta
      841 taacgaaatg ttaggatttt tgaacttctg caagactaac gattgtctgc ccgatgttat
      901 aacgtggcac gagcttcagg taagcgattt aaataatctt aatataaata atacaaacaa
      961 ttctattcag cgttaccgct ctgcggcaag agatgcaggg gttgaggaaa agcaggttgt
     1021 aattaacgag tatgccgact ttgccgactg cggtgttccg ggcaggcttg taaactggat
     1081 tgcgcgtctt gaagataatc aggtttacgg atgccttccg ttctggcatc aggcaaataa
     1141 tttaaacgat cttgctgcaa acgccaatca aggcaacgga gcttggtggg tatacaaatg
     1201 gtatggagat atgtcgggaa agactcttgg tgtagagcaa agcaacacat ccgtagaggg
     1261 attttacggt gtggcgtcct acgataagaa caagcgcagc gcgagcgttc tgtgcggcgg
     1321 aacggacgga gaagctatag ttaaacttga taatatttca tcaacgtcgg ttttcaaaga
     1381 tgccgataag gttcatatta aagtcgaggc ttcatatttc agcggctatc atggtgcggt
     1441 atatgctcct gaaacagtga tggaaggcgt attccctgtt aacaatggaa gtgttacaat
     1501 aaatcttgag gatatgctgt tttcagcggc atataacata acgattacag aaacaaccga
     1561 agatgtaagc gaaccggcag taggagcatt tcgcagtgag tatgaggcag aaaatgctac
     1621 cccgtatggc agccttataa tcgacaatca ggatactcct cttgaagagc cgcgctattt
     1681 ctgctcgggt ggagttcgta tcggcggaat tgaccaaagc ggcgatggta tagaatataa
     1741 cattgacgta cccgtagacg gacattacag gcttgatttc ctttatggaa acggtgttgg
     1801 ctcaacaaga aacaatgcta atacgcatag tcctaaaaat attacacaaa aacttgtaat
     1861 agacggcgcg gaaagtgagc ttttactacc caatacactg ttttattcca tggaggggat
     1921 ggttccccaa tatgctgacc tcaaagcagg gcatcacaag atagcgttga tgtataacgg
     1981 cgaagcgggt ggatttcacg atgcgctata tgtgtcctat acaggcgcat atggtgaaga
     2041 atatcctgaa tttgataaaa tatacgaggc ggaatcagcc gatttcaatg cgctcggaga
     2101 aacaaaagag agctttgnnn nnnnnnntac agctcaaatg gctatgtaac aagacttaat
     2161 ctcttgcctg taacaagcgg cggcggaata cgttggatag ttgatgtcga ggaaagcggt
     2221 ttgtatgacc ttgaatttaa ggttaattct gctaatactg gtaaactgaa tgtgtacatt
     2281 gataatacaa acctgacgtt tgacaatttt gctgttaata tggatattaa accgaataat
     2341 gactggcaga aatatggagc tacgcttttc ctaagacagg gaataaatat tgttgatatt
     2401 gataccgact gtgaagcggc agtggattat atgcatgtta tacgctcgga cagcaatccg
     2461 gcagtggtcg tcgaagcaga ggacgcgcag ggcaagtttg aaacggcgat ttcaggcgat
     2521 gcaacctatg ttaaggaaat gcgcggagac gagtatcttg aaatgaccgt gaacgctaag
     2581 caagatggtc tgtacaaaat gcaggtgttc cagtccaacg atgacctgtg cggaacgcac
     2641 agttacaata ttaaaattat agaccgctac gcgactgttg aggtaaacgg tgaacaatcg
     2701 acacgatact ttttccctaa ctcattttca gacgatacct tccttgaaag gactgtaccg
     2761 attgagttaa aagcgggaga aaataagata agaatttata atgatgacag ctggcacgtt
     2821 aaatggggag gtacgacgtc aacgcccggt acaaatgaac tcgtaaacta tacgccaaac
     2881 tttgataaat tcatcataac accggcttcg attcaatggg aggcaaagga aagatcaaac
     2941 acaatatttc tttcgtctac aaaaaacgga tatatttact gtgacaagaa cacggcttat
     3001 aatggtgaaa ccgcaacggt atatcttgtg cctgacgggg aggtaaagag ccttacttta
     3061 aatgggaaaa atataacaca gactctttca acagaggatt atattatata taagactgaa
     3121 attgagatta acggagacac aaatctgtat gccgagttta cacctgctat agaaggcgat
     3181 tttgataagc cgataataga aaatccggga acagtcgtta tagacggtaa aaaatataag
     3241 aaaataggag aaaacctttt caaaaatcac aactttgccg actacagcgg cgcggatatg
     3301 gatcagtggt atgtaggtgt aaataacgga ggacacccta cggcggaaaa ttatcagata
     3361 ccaaagataa atccggacgg aagtgtagaa aatcttgttc ctatcgccga gagcggactt
     3421 cttacaaccg gaggatatga gaagaccggc gaagatacat tctattacgg aaaagacaag
     3481 gcgggacgta catatctggt tgaacaactg agcagcgatt ggcaaaactg tgcgtggaac
     3541 ggagcgcatt cgcttttagg atttatccca ataaagccga ataagaatta ctatttccaa
     3601 tttgaagcat tttcttctaa aaagtctaat aaaaatacaa aagcatctgt acgttacggc
     3661 gccataaata tggaaaactt tgttccgaaa aattatacgg caagcggtac gctgaatttc
     3721 agcggtaccg gttatatgac gtgtaaaaac ggagatgagc tgaatatagg gggaaacagc
     3781 tgggaaacat atgatgctct gataaacagc ggtgacggcg actatttcct ctttaatgcg
     3841 tattggcttc atatggcgca ttatatgtgt tttggcgact ttcagcttta tgagctgtca
     3901 gatacgccta tggtgaatgt ttcggagctt aaaaatccgg cggcattggt gcttaatcta
     3961 aatgataaac tgtcgcttgc cgataaagct tcaattgttg ctgatgatgg tgaaacctcg
     4021 gaactgccca ttacatggct taatgccaac ggagttgaca taacaacccc cggtgtctat
     4081 gttgttacag gatatgtcag cttaccggat gattattata gtgataatgt atatgttaag
     4141 cagaaagtaa ttgttaaggg tatacctgca gctgaaataa ccaaaacaag caaagacggt
     4201 aacaaggtcg gtttctgtgt aaatgtgaat agcaattgtt ccggtgtgtt gctgtaaaag
     4261 acgacgacgg aaaactctta aatgtaaaat caaaagaaat tgagcttcaa acgggtgaaa
     4321 cctatgatgc ggaatttgaa tttgcgggtg aattaaatac ggtcgagatt ttcttctggg
     4381 aaaaggatac aatgagaccg ttctgccata aggttacgat ataacttagt aatattgatt
     4441 ttgggagaag attatgaaag atataatatt taacggggtg ccgtggtttg atcaaaacgg
     4501 gaataccgta aatgctcacg gtgcatgtat agttcgggaa catgatactt attatctgtt
     4561 cggggagtac aaaactgatg acaagaatat gtacaacggt ttttcgtgct attcttcaaa
     4621 aaatctggcg gattggaaat ttgaaggcat ggcattgaca ccggacgcag ataaaatgct
     4681 gtaccctgat cgtgtcggag aacgtgttaa ggtagtgaag tcaagaaagg cagaaaatta
     4741 cgttatgctc atgcatacag ataatatgca atacagcgat ccgtgtatag gagttgcggt
     4801 cagtaataag ataaatggca gatataaatt tatagggcct cttatgtaca agggtgaaaa
     4861 ggtgcgaaaa tgggatatgg gaacctttat tgacgaggat ggaacagcat atctgatgac
     4921 tcatgaaggc aacatatacc nnnnnnnnnn acatataccg cctcaatgat gaatgtactc
     4981 aagcagagga gctggtcgcc gaaaacatat ctcccggcgg agaatctcct gcaatgtgta
     5041 aacatgatgg taagtattat attatgtttt ccaataaaac agggtgggat aataatgata
     5101 actactacct taccgctgat aatatcaatg ggccatggga aaaccaaggt ctattttgtc
     5161 ctcagggaag cgctacatat aatacgcaat gctcctatat atttgatttc caaattgaag
     5221 acaaaattat aaaaatgtat ttgggagaca gatggtcgta tcctaaacag gcatcgtctg
     5281 caacactggt gatgttgccg ttagagtttg aaaacggaaa aatgtcgata agtgaatata
     5341 tgcaggcatg gtcagctgat gatgtgagtg aatttaaaga acaacaactt atatcatata
     5401 aattttactc aaacaaaaaa tcagaaagca gagaaattca gttttgcggt gaaagaatat
     5461 ctctgttcgg caaagtctca aatgaaggcg ggtattgtag gcttgacatt atggataggg
     5521 caaataatat tgtcaagaca gatattataa acttttatgg tactgcgcct atgagcggtg
     5581 tacggtatgt ttcgccaaaa ttggagagag cagattatgt actgcgtatt acagtgttga
     5641 gcgaaagtgg tgaatggttc acaaaggacg gtatgcgata tggcagccaa gattgttatg
     5701 tagatattta cggttactct gtagatgatt tttaacgatg cagaatgcag atggttctat
     5761 aatgcgtttg ccagtcaaat tgtaagcgtt cttgttgata cgaagagcat gaagattacg
     5821 gcaaaatata cttccgaagg cgcattaacg gatataaaaa tcgaaaaggc gaatatatcg
     5881 gaaataacag ctccgataaa taccgcgaca gaaaagatat tctattggga gtcatttgaa
     5941 agcatgaagc caatagaact aaaatagtat aagctatatt catattagat ggggagccgg
     6001 tagaaatatc ggttccctgc aataattatc atttgcagaa aaaacttctt taccataatc
     6061 gtttcgattt cttctctctt
//
LOCUS       DAWACS010000035         6023 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1465_length_6023_cov_2.518097, whole genome shotgun sequence.
ACCESSION   DAWACS010000035 DAWACS010000000
VERSION     DAWACS010000035.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1465_length_6023_cov_2.518097"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    226..235
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1560 a         1504 c         1006 g         1943 t
ORIGIN      
        1 attatgaaca tcatcagccg tgacttcttt ttgtgtcccc gttatacgtt tttgattgca
       61 aaaaacacaa tcaaacggac aacctctgtg cggtacaaat atcggtatat tatacgtttt
      121 catagtcact gtacttttcc cattgattta agcaaagtat gtgccgcatt ttgttcagcc
      181 tcttttttgc tgtgacctgt tcctttattt ttaggaacat catcgnnnnn nnnnncactt
      241 tgttgtgatc caaaccggat tcgccgattg ttatgtatgt gactttgccc tgtgtacctt
      301 tttgaagtgc ctcttgcagc attgttttat agtcggcata tccgtgacct gtcacaactt
      361 cgttaatcga atcacgcata aggtcgatta cccattttct tgccgtttcc ataccgccgt
      421 caagataaat tgccgccaaa actgcctcaa atgcgtctga aacgatagac gctctctgac
      481 gtccgcctgt catttcttca cccttaccaa gcaatataaa tttgctaagc gaaattttct
      541 tagatatttc gttaagcgac tgttcgcaga caagcgttgc acgatatttt gaaagccagc
      601 cctcgtcaac attcggcaaa cgtctgaata tatagtcact gattataaca ctcaaaacag
      661 aatcgcccaa aaattcaaga cgttcgttat tttctttttt acactcattc gcatatgagc
      721 tgtgtgtaag tgccgtttct aaaagattga catttttaaa tgtgtaaccg attttctctt
      781 gtacgttttt cataacagct ttcctttctc tgtacataca ctgctctaaa gagtgaaccg
      841 ttcaccgctt agagcaatcc acataacagt agagggtgcc ttaaaaagac atcctctatt
      901 gtttaagttt atttaattaa tcttcgtact ttttcattac gatagttgcg ttatgaccgc
      961 caaatccaag tgaatttgac attgcaactt taacttcctg ttctctgccc ttattcggaa
     1021 ctatatccaa atcacaagca ggatcaggtg tattgtaatt gattgttgca ggaatgtaac
     1081 tgtccttgat agccaatgca caaacgattg cctcgacacc gcctgccgca cccaaaaggt
     1141 gacctgtcat tgactttgtt gagcttactg caacgttctt agccgcgtcg ccaagagcct
     1201 cttttatggc ttgtgtttca aactcgtcat tgtactttgt tgatgtaccg tgagcattga
     1261 tgtatgttac atcttcaggc tttaaacctg cgtcttcgat tgcaagtgac atagcctttg
     1321 caccgccgac accgcccgga atagggcttg tgatgtggta agcatcatca gttgcaccgt
     1381 agccgacaag ttcacaataa atgtgagctc ctctcttctt tgcgtgttca agttcttcta
     1441 taactataaa gcctgcacct tcacttagaa caaatccgtc acgttcagcg tcaaacggtc
     1501 ttgacgctgt tttaggatcg tcatttcttg ttgacatagc tttcatatta cagaagcctg
     1561 caaatgcaag cggacttaca gccgcctctg caccgcctgc gatgataaca tcgttagcac
     1621 cgtgctgaat gtgtcttaaa gcgtcaccga tagcgtttgt acccgaagca caagctgtaa
     1681 cgatattttc atttacacct cttgctccgt acttaatagc aatctgtgca gcgcccatat
     1741 tacctatcat cataggaata aagaacggac ttacacgtcc cggacctctg tcaagaagtg
     1801 acttatgctg ttcctcaaga gtatggatac cgccgatacc cgaacctgtt ataactccga
     1861 ctttccaagg gtcttcgtct tccatattaa gacccgaatc cttaacagcc tcatcagccg
     1921 ctaccattgc aagctgagta tatctgtcca ttttacgagc gtctttcttc tcgataaaat
     1981 cagtcggctc aaaattctta atttcaccgg caatctgtgt cttaaaatca gttgcgtcaa
     2041 aaagtgttac tttatcaata ccgcatttac ctgctttgat accgttccaa aattcttcaa
     2101 cgttatttcc tataggggtt acagcaccta agcctgttac aacaactctt ctcatttagt
     2161 tacctcctat tgtttatcct tatctatata ataaaattaa tttctttttt caaaaaaatt
     2221 tcatgccttg caccaagagt attgatacaa ggcacgactt cgtatcttac gcatgactct
     2281 taatatattc aactgcgtcg cctactgtgc tgatcttttc agcatcttca tcagggattt
     2341 cgatatcaaa ttctgtttca agacccatga taagttcaac aacgtccaat gagtctgcac
     2401 ccaaatcgtc aacgaatgat gcttccaaag ttacttcatc tgcatcaaca cttagttggt
     2461 caacgataac gttctttact ctttcaaatt ccattattca cttgcacccc ctttcagtaa
     2521 tgtctatata aattctaaac gaattatata catattatat tacataacaa gtccgccgtc
     2581 aacatttatt acttgacctg taacgtattt tgacatatca gatgccaaaa atgcaacaac
     2641 atctgcaata ttttcagttt ctccgaattt tccaagcgga attgagtcaa gatatttctt
     2701 ctttacatca tcagacaata catctgtcat agcagaacgg ataaagccag gtgctactgc
     2761 gttacatctg atacctcttg atgcaaattc ctttgctgtt gtctttgtaa gaccgataag
     2821 acctgctttt gaagcaacat agtttgcctg acccggatta cccataacac ctacgattga
     2881 cgccatattg attatagcac cgcttctttg tttcatcata ggtcttgcaa ctgccttgat
     2941 acaattaaat gcacccttta gatttatatc cataacaaga tcccaatcgt cctctgacat
     3001 tctaatcata agaccgtctc ttgtaatgcc tgcgttatta acaaatatgt cgatatgacc
     3061 gaatttatcc atagtttccg atacaagttt ctgaacatct tcggtatttc ttacatcgcc
     3121 tttaacaact atacattcta caccaagctt tttaacttct tcacaagttt cgtctgcata
     3181 atccgatgaa ggaatatcgt ttataacgat atttgcaccg tgtgacgcca gttttagggc
     3241 gattgattta cctatacctc tgcccgagcc tgtaattacc gctgttttac cttttaacat
     3301 ttctttatct cctctctgtt atgcgttaag accttcaaga gtcttttcaa gtgacgccat
     3361 atcctctacg ttaaacacct ttacatctct tgaaactttc tttataaatc cgctaagtgc
     3421 cttacccggt ccgacttcaa cgaatgtatc aactccgtct gctatcattc ttctgatagt
     3481 ttcttcccat ttaacagatg aagatacttg cttgattaaa agcggtttaa tatcttcctt
     3541 tgaagaaacg taatcagcag ttacgtttgt gattacagga atttgcatat ccttaacttc
     3601 tacattttca agttcttttg caagtttttc gcctgcgcct ataagcatag cacaatggaa
     3661 cggagctgaa acaggaagtt ttaaagcacg ttttgcgccc ttttcctttg ccaattcaca
     3721 acacttatcc actgccgcaa cttcacccga tacaacgatt tgaccaggac agttaaagtt
     3781 tgcaggtgaa caaataccga cttttgacgc ctcttcgcag caagcgataa cgtcctctgc
     3841 ggaaagtgcg ataatagccg ccatagcgcc ttttcctaca ggcacttcct cctgcatata
     3901 cttaccgcgt ttttttacaa gtcttacacc gtcggcaaat tccattgaac ccgacgcaat
     3961 gtgtgcagta tattcaccaa ggctaagtcc tgcaacaacg tcaggcttaa tacctttttc
     4021 ttgcaatact ctaagtgccg caacgctcat tgtaacgatt gtcggctgcg tgttctctgt
     4081 aatcataaga gtttcactgt cgccgttcca aatcatatcc tttatatcaa agccaagtgc
     4141 ctcacttgcc tcgtcaaatg tcttatcggc aatcgggaag ttttcggcaa gttccttacc
     4201 cattccgaca gcttgtgcgc cttgtcctgc aaatacaaat gctaatttac ccattatatt
     4261 tattttcctt tcttaattta tctaataaga aattcttatt taagtttaga agtaattgtt
     4321 tcaataactg cctctgtacc gtcaaaataa ctcttgatta tgtctgcaca aggttgaatt
     4381 tcgtgtacca tagcggctga ttgaccagcc ataagtgaac cggtttgtgt atctccttct
     4441 tcaaatgcac gtcttagacc gccaacgcca agtgcttcta tttcttcaaa tgacgcacct
     4501 tgcttttcaa gtttgtcata ttcacgagta agtttgtttt taagcgctct tacaggcgca
     4561 cctgttgatc tgcccgtaac aaccgcatct ctgtcttttg cctttataac ggcttgctta
     4621 tagttatcat gtgcgataca ctctgttgag cagataaatc ttgtaccgac ttgaataccg
     4681 tctgcgccaa gtgcaaatgc cgcaacagca cctctgctgt cagctatacc gcctgccgct
     4741 acaaccggaa tacttaccgc gtcaacaacc tgcggtacaa gtaccattgt tgtaagctca
     4801 ccgatatgtc cgcctgcctc tgtaccctct gcgataattg cgtctgcacc gtctttttcc
     4861 atcttctttg ccattgcaac acttgcaaca accggcataa tcttaattcc tgcctcttta
     4921 agtgacgcta tgtatttacc cggattaccg gcacctgttg caactactgc cggcttttct
     4981 tctataataa cctgcataac ttcttcaacg aacggtgaca tcatcattac gtttacgcca
     5041 aaaggcttat ctgtcagtga ctttgccttt tgaatttgcg ctcttacaac gtctgccggt
     5101 gcatttcctg ccgcgataag tccgataccg ccgccgtttg aaacagctgc cgcaagttcg
     5161 gctgtagcta cccacgccat accgccttga ataataggat attcaatacc aagcaattca
     5221 cataatcttg tcttcatttc tcttctccct ttcttattta acgtatttgt tttaccactc
     5281 aataagtgcc gcaccccatg taagtccgcc gccgaagcct acaagtacaa ccttgtcgcc
     5341 ctttttaatt ctgccttgct gaactgcctc tgtaagtgca acagggatac aagacgctga
     5401 tgtgttaccg tacttttcaa ggttcaaaaa taccttttca tcagtgatac ccattctctt
     5461 tacggcactt tcaacaattc tgtagttagc ctggtgaggt acaacaagtg caatatcgtc
     5521 ccaagtcaat cctgcactgt ttacaactct tgcgcttgcg tctgccattg ctttgacagc
     5581 aaacttcata acagcttgtc ctgccatcca aatagtatct tttctgtggc ttattctctt
     5641 ttctgtttct tcatcatcat ttctgtatgc aagacttgtt atagcgtgac cgcttgagcc
     5701 gtctgcaccg atgtctgttg cgataatacc ctgttcttca cttgcagaca caactgccgc
     5761 acctgccgcg tcaccgaaca atacgcaagt tgctctgtct ttataatctg tagccttgct
     5821 gaatgggtcg gcacatacta caacgatatg cttgtatgtt cctgctttta taaactgttc
     5881 tgcgatagta agacctgtaa caaatcccga acaagccgca ttatagtcaa atgccgccgc
     5941 atttaccgca ccaagattgc tttgtacaac acaagcacaa gccggcgtga agtaatcagg
     6001 tgtaactgtt gcaagcatta taa
//
LOCUS       DAWACS010000036         5976 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1483_length_5976_cov_2.462760, whole genome shotgun sequence.
ACCESSION   DAWACS010000036 DAWACS010000000
VERSION     DAWACS010000036.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5976)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5976
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1483_length_5976_cov_2.462760"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5288..5297
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1940 a          974 c         1403 g         1649 t
ORIGIN      
        1 catattcctg ccgcaagatt gaaagaggaa gaaatggaga gattaaaaaa tcttgaaaat
       61 attcttcacg caagagttat cggtcaggac gaggcagtaa gtgcggttgc gaaagcaatc
      121 aagcgtggca gagccggact taaagaccct aaacgtccta tcggttcatt cctgttctta
      181 ggtcctacag gtgtaggtaa aacagaactt tcaaagactt tggctgaagc gatgtttggc
      241 agtgaaaatg cacttatccg tgtagatatg tcggaatata tggagaagca cgccgtatct
      301 aaatttatcg gctcacctcc gggatatgta ggatttgaag agggcggtca gcttactgaa
      361 aaaatcagaa aacaccctta ttcggtaatc ttgttcgatg aaatcgaaaa ggcacatcct
      421 gacgtattca acattatgct gcaaattctt gatgacggta ttcttacaga cgcacagggc
      481 agaaaagtag acttcaagaa cactgttatc attatgacat ctaaccttgg tgcaaaagaa
      541 attctcggca atgtatcatc taaacttggt ttcaagaaag acgaggaaaa ggacaagaac
      601 atcagtgaac acgatagtat caagaataaa gttatggaag aagttaaacg tgcgtttaaa
      661 cctgaattcc ttaacagaat tgacgatata atcgtatttg acagattgac agaggataac
      721 atcaaggaaa ttgcaaaact tatgcttaaa tctcttgaaa aacgtttgaa tgcgaatgaa
      781 atcaatgtaa catttacgga gagtgccgta agtaaaattg ctaaatcggg ctttgaccct
      841 gtatacggtg caagaccttt aagacgtgca attcaaaacg aaatcgaaga tatgctttcg
      901 gaagaaatca ttgacggtaa cattaagagc ggtgacagca ttactgtaga tgttgaggac
      961 gataagttta ctgttaaaaa ataagactca aataaggcag tcacattttt gcgactgcct
     1021 tatttatgca aaaaaatata gttgctcatt tgagcaacta tataaatttt tgtatttaat
     1081 tatgcagccg gagtagctgt cggagcagct gtttcagccg gagcttttgt cggtgctaca
     1141 gtagcagatg ggtctgcggt agcctcagga gcaacagtag ctgttgggtc tgcagtagcc
     1201 tcaggagcaa cagtagctgt tgggtctgca gtagcagttg gtttaactgt ttcggcagga
     1261 gtatcagtac cgtttataac aacttcagct tggctgatct ttagatcatc accttcatat
     1321 tgtactgatt tattgcctac attgctaact gagtcaacaa gaagtgatgt gttgtaacca
     1381 agctttgtgt tgtatccgtt tgaaagttgg aatgaaccct tttcgcctgt aagtgactta
     1441 aatgtaagtt tcataacagc accgtctgtc ttaactgctg tttcggcatt aacagtaaca
     1501 gatgcggctt ttacataacc gtatgtaaat tcagggtttg ctgcaccgat tgcaccttcg
     1561 attacgttgt cgccgtttac aagttcggct tttacgaatt caaatgcagt agggtcatac
     1621 ttaacagttg cagttacgcc tgaaacaaat ttttcagtcg gaagattttc tgcgtttaca
     1681 gaaagaacaa cttcttcgtc cttagcggct gttagcttgt cagctgaaag tgacaatgca
     1741 ggaacgcctg cgtctgctga aggagcgtta gctgttgtga tattgattgt gtaatcttca
     1801 ccgttccatt gaacatctgc gtcaagagcc tcgctgattg ctcttagagg aatcatagtt
     1861 ctgtcgctga tgatttgagg agcaacgtcc aaagttacgt cattttcagg agcaacgaag
     1921 tcttgtcctg acataagagc tccaaaaaca ccgcttacgt caaaaacttt cattgtactg
     1981 tcgtcgatag tcaatctgat aagagttttg ttatcagaac ttgtaacgtc aacagttctt
     2041 gtatcttctt tccattcaac ctttgcaccc atagcctcaa atacgcctct tgcaggaaca
     2101 agagtagtac cttcgccaag aattacagga gcttggtcat caaaaaagat ttgaccttgg
     2161 tttacgaata cagtaggagt tttatcctct gccatagcaa tacctgtgca tgaaactgca
     2221 agtgcagctg caagagaaat gcttaaaatt tgtttcttca taaaaaatac ttcctttcat
     2281 tttacgaatt tatcaaacat tataacacaa aaactcgaaa tatacaataa aattttttaa
     2341 atttgtatag ccatacaatc gaaaatgtag tataataata aacaactgag gtcatataga
     2401 ggatatatcg ccttaagctc caagaaaatg aaaggagtat ctgaatatga atgcgttttt
     2461 ggaatactta gtaaaatatg tacagttgct tagaataagt gatttaattg acatattgat
     2521 agtttcggtt atagtgtatt atcttataaa tttaattcgt gaaacaagag caatgcagct
     2581 tgtgaagggt attatatttt tgtttatggc attcttttta agtcagtggc tgaaacttaa
     2641 tgctttaaac tatattttag gcggtgcaat gcaaatcggt gcatttgcgg taatcgtaat
     2701 atttcagcct gaattgcgta atttgcttga aagaatgggg cgtttcaaag tcggacgtat
     2761 aattgatttt gcgtcggaca ctacagagga cgatttacac agggttatag attcgattgc
     2821 acaagccgcc gcgaatatgt cggcgacaaa gacgggtgcg cttattgtaa tggaacgtgc
     2881 aacgcgattg ggtgaatata taagtacggg gacaatgctt gacgcgaatg tatcaagcgg
     2941 acttttggag aatatatttg taccgaatac acctttgcat gacggtgcgg ttataataag
     3001 gaataataaa attgtgacag ccggctgtct tttgccgctt accgcaaata ataatttatc
     3061 aagagatttg ggtacgcgtc acagagcggc gataggactc agcgaagtca cagacgcggt
     3121 tattatagtg gtgagcgaag aaacaggcaa gatttccata gcgttaaacg gctcgcttac
     3181 gcgtaatctt tcaagagagt cgcttgcaaa ggcacttaaa aagattatga gtcaaaagca
     3241 ggaaacgaac gaaaagatag ataagattat gttttggagg acgaaatgac ggagaagaaa
     3301 gcgaatgtaa cgaatactca aaagctgaaa accgtaatac tttcggtttt ccttgcagta
     3361 atcgtttatg gttatatatg tgaatgaccc cgatattacg actaccgttt cggatttgaa
     3421 tgtaagattt gtcggtgaaa tgtcgcttag ggataaaaaa cttgcggtga ccggtaagga
     3481 taaaattcac gaattgtcgg ttgttgtaac gggtaaacga agtgacctta tgaattttat
     3541 ggacgatata tatgttcagg ttgatgtcag cgatattgac tcaacgggtg aatataattt
     3601 gtcgggagtg atttcaattc cgacaacgag aatttcagtt gaaaaggaaa agtacggaga
     3661 tattccgatt acggttgaac cgcttgaaat gaaagatatt gaggttactg taaaacagac
     3721 cggtatgctc aaagacaaga tagtaaaatc gtctgtaaat aatccgactg tcacaataac
     3781 aggtgcaaaa agtgaaatag acgaggttgc aggtgctatt gcaacggttg atgtgtcaag
     3841 tattcaagag gacggcgtgg aaaacgtgaa ctatcttctg acggacacaa acggtgaact
     3901 tataaacaag aatgaaacga ttgaaagtgc acgttcttat gtggaggttg taaatacgat
     3961 atatgacgca aaacttttgc ctgttgtgcc aagactttcg gcagagcttg acaaggaata
     4021 tattttgaaa gcggataaat cttttgccac acctgcaagc gttacggttg gcgtggacga
     4081 aactaatacg gatgataaag tcattgcact tatagataaa gttccgtcag acggtttcgg
     4141 cgaatatgaa cttgaattgt caagcggtat gtatataccg gaggataata aaaaagtcaa
     4201 gtataaattg gatatagtaa aaaaagccgt tgcacagctt gaacttaccg tacaggcaca
     4261 aaatgtacaa tcaggtttga cggctaagat aaacaataaa ctcatcgcac aggtttgggg
     4321 tgaggaagga aaaattacca cagataatgt aaaggcttac gttgacgtaa gcggacttgg
     4381 agcgggaaca tacaatttgc ctgtaaaaat tgaaggtgaa aatgttagtt tcgcagaaaa
     4441 ttatacaata gaagttactg tagaatagga gaataaataa atggcagata aattagtaag
     4501 aggaaacagt atggacggtg caatccgtgt ttttgcggcg gtgacaaccg accttgtgaa
     4561 tgaggcacag agaatacatc atacttatcc ggttgcaacg gctgcactcg gcagattgct
     4621 tacaggtgcg gcaattatgg gtgcagcagg attgaagaat gaaacagaca gtatgacggt
     4681 tcagataaag ggcgagggtg aaattacaaa ccttgttgcc gttacggatt gtcacagtag
     4741 ggtcagaggt tatgtttcaa acccgtatgt tgacagaccg cttaacaata aaggtaaact
     4801 tgatgtcggc ggtgctatcg gcggcggata tttgagtgtt gtacgcgatt tgggattgaa
     4861 agagccgtat gtcggacaaa ttccgcttgt atcgggcgaa attgcggagg acttgacata
     4921 ttattatgca aaaagtgagc agatacctac atcggttgca cttggcgtgc ttgttgatac
     4981 agacaacagc gtaatagcgg caggcggttt tatgatacag cttatgccgg aggctaccga
     5041 agaaatggcg gttcagcttg aagatatatt aaaggagttg cctcctgtta cgacaatgat
     5101 taaagagggt atgactgcgg aggatattct gttcagagta acggaaggat tttcgatggt
     5161 atgtgaaaac aaggcggttg taccggaata taagtgtacg tgttcaaaag agcgtatgga
     5221 aaaagcactt atttcaatag gtaaaaaaga gcttgaagaa ctgattgagg atcaggggga
     5281 ggctgaannn nnnnnnnggg ggaagctgaa cttacttgcc agttctgtga taataaatac
     5341 catttcaata agaaagaact cgaagattta ttggaaaaag caaaataaat aaagaattta
     5401 gtaaaaaatg cttgacaatc cgagagaaaa tctatataat atttgaggtg tctattttta
     5461 tgataggaat aaagacggct gattggggcc ggaattaagc agaggaggtg ctgtaacaat
     5521 gaaaagaaca tttcaaccga aaaagcgtca aagagctaag gaacacggtt tcagaaagag
     5581 aatgtctact aagtcaggca gaaagatttt ggctagcaga agacagaagg gcagaaagaa
     5641 gttatctgca taatatatga agatgtaggc aggaagcggg gaggaattct ttgcttgttg
     5701 cctttttttc ataataagac aaatgaggtt taatatgaaa aacactcaaa gtttaaaact
     5761 caacagagat ttcagacgtg catacaaggg tgataacttt gtcggaggat ataccgttgt
     5821 atatatgaag aaaaataaat atcagtttaa ccgtcttggt ttgagagtcg gtaaggcgac
     5881 cggtaaggct gttacgcgaa atcgcttgaa aaggcttatg cgtgagagtt atcgccttat
     5941 ggaagatgat attgaaaagg gttatgactt cataat
//
LOCUS       DAWACS010000037         5906 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1506_length_5906_cov_2.665185, whole genome shotgun sequence.
ACCESSION   DAWACS010000037 DAWACS010000000
VERSION     DAWACS010000037.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5906)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1506_length_5906_cov_2.665185"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5435..5444
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1627 a         1320 c         1047 g         1902 t
ORIGIN      
        1 cctttgcagc acctgttgag ttaggaacga tgtttacagc agcagctctt gatcttctta
       61 gatcaccctt tctttgagga ccgtcaagag tcatttggtc acctgtgtaa gcgtgaattg
      121 tgcacatgat acctgactga attggagcgt acttgttaag tgcgtctgcc ataggagcca
      181 agcagtttgt tgtacaagaa gcagctgaaa tgatgttgtc gcccttcttt agtgtttcgt
      241 ggtttacgtt gtaaacgatt gttggtaggt cgttgcctgc aggagcagaa ataacaactt
      301 tcttagcaca tactttagta gatgagcaag catctttggg cttgtaaggt cgttgatagc
      361 aacaacttca taaccctctg cgccgaacat ctgtctgaat gcaagacgtc caatacgtcc
      421 gaatccgtta atcgcaactt taactgccat tttaaattcc tcctaaaaaa tatatatatt
      481 tttttatctt tgagtgagca tagtatatgc ccatttatat atattttaca tcaaatttat
      541 aaaatataca agcctatatg ttaaatttat atcattttaa tgattaattc ttccttttgc
      601 acaaaaagca gcctatttat atcagtttta tatataattt ttttaaacaa aatgaactgc
      661 tgtgaaaatt cacagcagtt cataattatt ttattctatt tcttcgttgt caacttcttc
      721 gccgtcgata gtaatattaa tatctttata catactcata cctgtaccgg caggaataag
      781 tttaccgatg ataacatttt cctttagacc aagaagcggg tcaaccttac ccttaatagc
      841 tgcatctgta agaacctttg tagtttcctg gaatgatgcg gctgaaagga atgattcggt
      901 tgcaagagaa gccttagtga taccaagaag tacaggagat gcagttgcaa attcagtacc
      961 gcccttttcc ttaactctct cgtttgcctc ttcaagttca aacttatcaa caagtgaacc
     1021 gataagaaga tcagtttcgc ctgcctcttc aactctaacc ttacgcaaca tttgacgtgt
     1081 gataacttcg acgtgcttat cgttgatgtc aacaccctgc atacggtatg ttctctgaac
     1141 ttcacgcgcg atataaacct gaacggcatg aggacctctg atatgaagaa tatcattagg
     1201 gtttacagaa cccattgtaa gttcgtcacc cgcctcaata acgtcaccgt catgaacttt
     1261 aatacttgaa ccgtatggaa ttgtataagt cttagcctca cctgtctggt cattggcaac
     1321 tgtaacttct ctcttacgct ttgattctgt tacagtaatt gtaccgccga tttctgagat
     1381 tatagcaaga ccctttggac gtcttgcctc gaaaagttcc tcgacacgag gaagaccctg
     1441 tgtaatatcg ctgcctgacg caacaccgcc ggcgtggaaa gtacgcattg taagctgtgt
     1501 acccggttca ccgattgact gtgcggcaat aataccaact gcctcaccga cattaacaag
     1561 ttcacctgta gcaaggtttg taccgtaaca tttagcacaa acaccgattt ttgacttaca
     1621 agtaagtacg cttctgatat atactttttc gataccggca ttaacaatat tattagcctg
     1681 tacgtctgtt ataagttcac cgttctttgc aagaacttcg cctgtttcag ggtgaataac
     1741 atcctgcata gctgtacggc cgacgatacg gtcatatagc ggttcgatac tttctttacc
     1801 gtcagtgatt tcagttaccc attcaccttc atcagtaccg cagtcgattt cacgaacgat
     1861 aacatcctgt gatacgtcaa caagacgacg agtaaggtaa cctgagtcgg ctgttctaag
     1921 agctgtatct gtaagacctt tacgcgcacc gtgtgatgaa atgaagtatt caagtacgtt
     1981 aagaccttct cggaagttag cacggatagg aatttcgact gttctaccct gtgtatccgc
     2041 cataagtcca cgcatagctg caagctgacg aatttggtta acgctaccac gggcacctga
     2101 gtcagccatc atgaagattg gattgaactc accaaggtgt tccatcatgg cgtctgtgac
     2161 atcagatgaa gcctttgccc aaattttaat tgtctgttga taacgttctt catttgtaag
     2221 aagacccatc tgatacttct gcataattcc ttcgacttgc ttttcagcgt cagcaaggat
     2281 aacttgtttc ttttcaggaa cttcaatatc tgatacggca acagttatag cacctcttgt
     2341 agaatactta taacctgtag ccttaatctt atcaagcact tcggctgttt ctgaagtacc
     2401 gcaaactctg atacacttat caataatttt accaagctgt ttcttaccta caacaaagtc
     2461 gatttcaagg tcaaattcat tttcaggatt tgatctgtca atataaccga tatgctgagg
     2521 aatattatta ttgaatataa ttctaccgac agttgtaccg ataagttttg acttttctac
     2581 tccgttaatc ttctttgtaa cttcaacaag aattctttcg tgtagagtta cttcgtgatt
     2641 ataataagca agaagtgcct catcaaatgt agtatacttc tttattacaa gtttcttagc
     2701 ctcacttatt aatttcttta gtgaaatttg aagtgattta ttcggtaata ccaaagttac
     2761 aggatttgcg tgaatttctg tttcattcat tgcaacttcc ggcaactctc tcatatattt
     2821 aagagcctct cttacatcag taaagctctt gattggttct tcttcattgt atataacaac
     2881 gttttcttcc tgttccatat cagaaagtct ttcgattaat acattgattt taggttcatc
     2941 atcaagaatt gtatcaataa ttctgtcata atgttccttt tgaattgtca agtagtaaga
     3001 acccaaaatc atatcctgtg taggaactgt tacagggtgt ccatcctgcg gtttaagcaa
     3061 gttatttgct gaaagcatta ggaaacgtgc ctcggcctgt gcctccggtg aaagaggaac
     3121 gtggatagcc atctggtcac cgtcgaagtc ggcgttataa gccgtacata caagcggatg
     3181 aagttttaga gcacgtcctt caacaagttt tggctcaaat gcttgaatac caagtctgtg
     3241 aagtgtaggc gcacggttta gaagtacagg atgctcctta attacatcct caagaacgtc
     3301 ccaaacctcc ggctttgttc tctcaaccat tctctttgca ctcttgatat tatgtgcaag
     3361 tccgcgagat acaagttctt tcattacgaa aggcttaaac aattcgatag ccatttcttt
     3421 tggcacaccg cactgataaa tcttcatttc cggacctaca acgataacgc tacgacctga
     3481 gtagtcaaca cgcttaccaa gcaagttttg acggaaacgt ccctgcttac ctttaagcat
     3541 atccgaaagt gattttagcg gtctgttacc cggtcctgtt acagttcttc cgcgacgacc
     3601 gttattgata agtgcgtcaa cagcctcttg taacatacgt ttttcgtttc tgataataat
     3661 atcaggagca ccaagttcaa gaagtctttt taaacgattg tttctgttta taactcttct
     3721 gtaaaggtca ttaaggtcag acgttgcaaa acgtccgccg tcaagctgta ccataggtct
     3781 aagttccggc ggaattaccg gaataacagt cataatcatc cattcaggtc tgttacccga
     3841 aagtctgaat gcctctacaa tttcaagtct tttaataatt cttgctttct tttgtccttg
     3901 tgtagcgtca agctcttcct ttaattctct tgaaagttct tcaagatcaa ttctttcaag
     3961 caaagtcttg atagcctctg cacccatacc tgctttgaac ttatcgccgt acttttcata
     4021 tgcctcacga tattcagcct ctgaaagaat ctgattttgc ataaggtttg aagtacccgg
     4081 atcagttaca atatatgatg caaagtaaag taccttttca agctgtcttg gagaaaggtc
     4141 aatgataagt cccattgatg acggaacgcc tttgaaatac caaatatgtg aaacaggagt
     4201 tgcaagagca atatgtccca ttctttcacg tcttactttt gcttttgtta cttcaactcc
     4261 gcagcgttcg cagacctttc ctttaaatct gattttctta tatttaccac agtgacactc
     4321 ccaatccttt gtaggaccga aaatcttttc acagaataat ccgtcctttt caggcttaag
     4381 agtacggtag tttatggttt caggctttaa aacttcaccg tgtgaccaac ttagtattgt
     4441 ctcgggagaa gctaaaccga ttttaattgc ctcaaagctg ttaaattcta acatctaaaa
     4501 acctccatag tctattcaca cgtcatgatt atatttcgtc gtcatcgccg aaatcaatat
     4561 catcagaatc atcaacgccg aaatcatttt cgtctgaatc atccaaaacc atacttgcat
     4621 cattaagatc atcttcatca aaagtatctg taattccgtc cttgtcctcc attgtttgta
     4681 caagttcttc gcttacaaca ccttcgtcct catcttcaaa tgatgcgtca aggtcgattt
     4741 cttccatatt atgattaaga atcttaatat caagtgcaag tgactgaagt tccttaacaa
     4801 gtaccttaaa tgattcagga atacctgact taggaatatt ttcaccctta acgattgcct
     4861 cgtatgtctt aacacgaccg acaatatcgt cagatttaac tgtcaggatt tcctgcaatg
     4921 tataagcggc accgtaagcc tccaaagccc aaacttccat ttcaccgaaa cgctgaccgc
     4981 cgaactgagc tttaccgccc aaaggctgtt gagtaacaag tgagtaagga cctgttgaac
     5041 gtgcgtgaat cttatcatca acaagatggt gaagtttcaa gtaatacatg acaccgactg
     5101 taacagggtt atcaaacttt tcacccgtac gtccgtcata tacgactgac ttaaagtcag
     5161 gtcttaaacc tgcttcttta aatgcaagtt ttatatcttc gtcctgtgca ccgtcaaata
     5221 caggagttgc aagtttaata aatcttcctt ctcttgcctt tgacttagcg tcaattatat
     5281 tttgtctgaa attatcgtca ataattgtat tttcaagctg ttcacgagtc tttaagctta
     5341 gacatctgta tgcgtagccc aagtgcattt caagcacctg accgatattc atacgagaag
     5401 gcacgcccaa agggttaaga acgatttgta gtggnnnnnn nnnnatcttc ctgcggcaat
     5461 actcttgaaa cgacaccctt attaccgtga cggcctgcca tcttatcgcc gacagaaatc
     5521 tttctctttt gagcgataac acaacgtaca acttcattta cgcccggtga caattcatcg
     5581 ccgttttctc ttgtgaactt cttaacatct ataattatac cctgttcacc gtgtggaaca
     5641 cgaagtgatg tatctcttac ttctcttgcc ttttcaccga aaattgcacg aagaagtctt
     5701 tcttctgctg taagctctgt ttcacccttt ggtgtaactt taccgacaag aatatcacct
     5761 gcgtgtactt ccgcaccgat tctgataata ccctgttcgt caaggtcctt tagagtatct
     5821 tccgatacgt taggaatatc tcttgtaatt tcttcaggac caagttttgt atcacgtgct
     5881 tcacattcat attcttcaac gtgaat
//
LOCUS       DAWACS010000038         5855 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1522_length_5855_cov_2.420862, whole genome shotgun sequence.
ACCESSION   DAWACS010000038 DAWACS010000000
VERSION     DAWACS010000038.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5855)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1522_length_5855_cov_2.420862"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    433..442
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2913..2922
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1864 a          894 c         1437 g         1640 t
ORIGIN      
        1 gtttataatc ataaaagcgt ctaaggttat gaactttgtc gcaaacaaaa taacttcgcg
       61 tgatgaaaca acatacaata ttgcgtattt ggcatttata tacatactta tgctgtcaac
      121 ggcggcatgg ggaatattta cgttttttgc ggatgagccg agaaaaatgc agatagctgt
      181 atttctgata tttatattta tattttgtga cgcggggtat ttgctgtact tcaattcgct
      241 gtacggagaa ccgctgcaat atgtgtcgct tatgatactt attgcgttgg ggcttttgat
      301 atacaaacga ccgactatac cgaaaatagc ttgctttttt gtggcactgt actttttcgc
      361 aggttcaaaa cttgcgaatg tgccgtattc ggttatagtt tcggtgcttg cactttcttt
      421 tgcgtatttg cgnnnnnnnn nnttaatact catgcttata tggatgacgg tgaatatccg
      481 attgatataa caacagacga atttcagcac gatttttacg acagaatatc aaaagcgaat
      541 gttgtgtttt tctatttaag acaccctgta aggttcgtaa agaaaatcgc tttttcgata
      601 gaaaacgcgt catgcttaag accgcttaat tcgggtaatt cagaaacggt gctaatgcag
      661 tattcaaacc gtttcagcct gtggagcaat ctgcgagtgg cgacaaagtt tttgtataat
      721 ccgtatattg tgtttgcaat ggcaattata atgacattgt atgttatatt tgtacacata
      781 tatcttgtta aaaatcataa agaaaccgat gaaaaaaggt tgtatatgat tatggcgatg
      841 tatgtgctta tagtcggatt gtggattaat atgtgtcttc cgattgtcgg aaacggtgag
      901 gcggatatta tgaaacatat gttccttttt gcaaattgta tggacgtgct ttttgctgta
      961 attattttag gaatagtaaa tatgcagctg cgtaacagga ttgcatcgat agcggcactt
     1021 gctgttgcgg taggagtatt gcagattgag ccgcctaagg aaactattga attcggaaca
     1081 tataacggtc agccgcttaa gtgggaagta atgcaggaat atgatgacgg cagtaaggtt
     1141 attgttacca aggattgcgt taccgaaaga atttttgatg acgaaaataa tatgtgggaa
     1201 acaagtgatt taagacagtg gctcaattcc gactttataa gcgagttcac aatggacgaa
     1261 cttgcaagaa ttgagccgaa ggaaaacgaa gttatgctta cttataatga cagaggactt
     1321 gcggtgagcg gtgatcatac tcattattgg agtgcgacaa gaagcgaggt tgcagacctt
     1381 tcggaaagtg cgtataagta ttacgttgat gatatggttt atattccgac acttgatatg
     1441 atgaaaacga tagatgttcg cggttcgtat tggattttat gcccatacgg ctataatgac
     1501 aaaatgcaga gatatatgaa gaatgatggg tttattcttc atacaaacgt agataatata
     1561 gatggagtgc gtgcggcagt acgaataaaa gcggagtaaa tatgaggtaa catatatgaa
     1621 aaatgaaata aatttgacaa tgctcacgga tttttatgag tttacaatga tgaatggctt
     1681 ttttgccgag ggatataagg ataaaattgc gtatttcgat atgtttttca gacgagttcc
     1741 cgaagacggc ggttttgcca taatggcggg tgttgagcag attattgaat atataaaaga
     1801 gattaacttt acagtggaag atattgaata tcttcggggt aaaggaattt ttaaagagga
     1861 atttcttaaa tatttggaga attttaagtt tgaatgtgac gtatgggcag tgcctgacgg
     1921 tacaccgata tttccgggtg agccgattgt tacggtaaga ggtcctgtaa tacaggctca
     1981 gtttgtcgaa acgatgattt tgcttacgat aaaccatcag agccttattg cgactaaggc
     2041 aaacagaatt gtaagagccg cacagggcag agcggttatg gagtttggct caagacgtgc
     2101 tcagggttac agtgccgcaa ctatcggtgc aagagccgca tatataggcg gtgttgcagg
     2161 tacggcttgt actatttccg atgaacttta cggagtgccg gcactcggaa caatggcaca
     2221 tagttgggta cagctttttg acagtgaata tgaggcgttt tatgcgtatg cgaagaatta
     2281 tccgacaggc tgtactttgc ttgttgatac ttataatgtg ctgaaatcgg gtataccgaa
     2341 cgcaattaag gtttttgatg aagtattaaa acccatggga gcaagaccta agggtgtaag
     2401 aattgattcg ggcgatataa cgtatttgtc taaaaagtgc cgtaaaatgc ttgacgacgc
     2461 aggttatccg gattgtcaga tagttgcgtc aaattcactt gacgagtata taattcgtga
     2521 tatgcttttg cagggtgcta agattgatat gtttggtgtg ggtgaaagac ttataacatc
     2581 taagtcagag cctgtattcg gcggtgtgta taaactggtg gcggttgaag ataatgacgg
     2641 aaatataatt ccgaagatta agataagtga aaatgttgca aagattacaa caccgggatt
     2701 taaacaggtg catagattgt attcaaagag tgacagtcgt gcgattgcgg acgttattac
     2761 aatgcacggc gaaactattg acaacaacaa accgtacaca atatttgatc ccgaacatac
     2821 atggaagaag aaaactgtta cggactttta ttcacgagaa ttgttaaaac cgatatttga
     2881 aaagggtaag tgcgtgtatg aatgtcccga ccnnnnnnnn nnaccttaat gtacttcgcg
     2941 aatattgtaa aatggagatt gacggacttt gggacgaggt taagagattt gaaaatcctc
     3001 atacttatta cgttgatttg tcggagaaat tgtggaattt aaagcataca atgcttgaag
     3061 aatatcaaga ttgacagagg ttaagaaaat aatggatacg aaattatttc atacagatga
     3121 taacgaaatt gccgaggcag gcgaaatatt gagaaacggc ggactggtcg catttccgac
     3181 tgaaacagtt tacggtttgg gtgcgtcagc ttttgattcg gacgcggcta agaaaattta
     3241 tgccgcaaag ggcagaccgt ccgataatcc gcttatagtg catatatgcg ataaagggca
     3301 gataaaggat attgcggagg aaataccgga aagtgcaaag aaagttatag ataattttat
     3361 gccgggaccc gtgacaatta ttttaaagaa aaaatcggtt gtacccaatg atgttacggc
     3421 aggacttgat actgttgcaa tacgttttcc gttacatgaa acggctcaga aacttataaa
     3481 agcggcaggt gttccgattg ccgcaccgag tgcaaatctt tcgggaaaac cgagtccgac
     3541 aaaggcaaag cacgttgtaa aggatatgac gggaagaatt gacgctataa ttgacggcgg
     3601 tgaatgtaat gtgggtgtgg agtcgacaat cgttgatttt acaggtgaaa agccggttat
     3661 attaagaccg ggcggcgtga cgtatgacga tttgaaaggc ataggtcttg acgttgaaat
     3721 agataaaaat atattacatt ccattgcggc agatgaagtg ccgaaatgtc cgggtatgaa
     3781 gtataaacat tatgcgccta atgcggaagt aaccgttgtt gagggtgaaa aagacgcggt
     3841 gcagagtaag ataaaggaac ttcttgaaca gaccgagggg aaagtaacgg gagtgcttac
     3901 aatgtacggt gcagtgtatg ataaagcagt gatgctttcg gcaggaagta caaataaaga
     3961 gtatgcaaaa aacttgtttg cggcattgcg tgaatttgat gaacttggcg ttgaagtggt
     4021 atttgccgaa ttttccgaaa aagacggcta cggacttgca gtgaaaaaca gattgtataa
     4081 agcggcggca cagagagtaa tacacgtcta aaaccgaaaa atcgcccaca gcaccacttt
     4141 ttgcttggac ggtacagtaa gtacagcacc aaagctgcaa agccacactg tgaacgattt
     4201 tgtggattta caactaaaag tctctccttt atgggaggta tcacgaagtg acggagtggt
     4261 ggcgaaatat gaatcccaca aaatacgaaa ggagatacga aatttatgaa tatattattt
     4321 gtatgtacag gcaacataca aacataaact ccgaaagcaa ggtaaaacca aaggaaaaga
     4381 ggattgtaat ttgcatttta cagaggtttt acaggatttg aagaaaaaaa gaaaccatag
     4441 tgtaaaaatt gaatatttga tatgaaaata agtctgtatt taaattatat ggacttattt
     4501 tttgtttaga gcagggaggt gttcaaatga aatgtctaat gagatacggt tgggtaaagt
     4561 taatgcgctc acatttaccc gagggtaagg gtatattggg aatgtgggca aaactggcat
     4621 cgcgtgcggc atttcgcaaa gggcacgcga cctattgtgg ttacaagaat gaagtgatag
     4681 ccggtatgtg gtctggagga attgttgggg ttaagagcat tttgggagtg cgaagccgta
     4741 ggaaagccct tgaaacgctt gatgcactat cagaactcgg atatattagg tacactcttg
     4801 atgtaaagac aaaaaagctt acatatcaga ttaccgattg ggttatccga tgctctggtg
     4861 aggaatgtag gaatggaaca gtttatacga cagacggata cggattttta tgtatgccgc
     4921 gcagtataac agaaagactc atagaacata attacatatt ggaagaagca gacgcatggc
     4981 ttgacctttg gtgtcactcg gtatcaaggg acagaaacaa tgcgttttct ttttttgcgc
     5041 cgacagtcca gcttggcaaa tacggtgctg tactgacttt ggaaacactc ggcaaacgct
     5101 ggggatggga aaagacaaag gtatggagat tttttaggaa atacaatgat atgttcgatc
     5161 tataccgctt gccgggttcg tatggatgcc ttgtgtttaa taaattgtat ccgtgtgata
     5221 cagagtgtgt aacgccttca caagagcaaa ttatgcgtat tcttgatgcg ataagagctt
     5281 atgcggtaaa tacaacaaag agagagacag accatcaata tatcaacagg cttattgcat
     5341 ggtacagccg gagagtggtt gataaatttt agacaactac cgatgaaaat cgcgttgcaa
     5401 aatcggacca tataatacgc gcgtatattt ctctgtgttg gaattgtaag aatatatatg
     5461 actgcaggaa tagttattgt attatggcaa cgacagaaaa caacgatatc cgtggaccgt
     5521 gcgtaccagc ggatttaaca gaaatagcaa aggagttttt tacctatgag taaacagaat
     5581 aaaaaatgaa taatttggag gtaaacgagc aaacaatagc aaattcagaa cggattatga
     5641 aaatgcttgc tcaaaggggt ataataagcg ataaaaacat tgatggggaa aaaatgaaaa
     5701 atgtaggaca ggagaagaaa tatcataaca cactgttgct tttaaagaat tacagaacta
     5761 ttgcttgggt gctcgaatgc tttcctgaca cgcttgccga ggaacttgag cagccatttg
     5821 aaggcttaga tgagcttttg gacagatttg atgca
//
LOCUS       DAWACS010000039         5790 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1546_length_5790_cov_2.640628, whole genome shotgun sequence.
ACCESSION   DAWACS010000039 DAWACS010000000
VERSION     DAWACS010000039.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5790)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1546_length_5790_cov_2.640628"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1881 a          956 c         1348 g         1605 t
ORIGIN      
        1 aaacgtagat attgcccacc ataaacccga aaaagcattt gaaaaaacat cgggctgaac
       61 agggttttca aagtaataca taataaccga agaaataatc atcaataaaa atacaacaaa
      121 tattgacgaa actaattgat cggatttttt ctttagtaca ttacctattg aagataatcc
      181 cgatgtatat cggttcattt tgaaaagtct gaaaaggcga agtaatctta ttaaccgcaa
      241 aattcttaaa tcaatgggta atagaaacgg tatgtaaaat ggcagtattg ctaataaatc
      301 gacaatagcc atcggagaaa agaacaattt tatgcgtgca atgaacggtt tcttttccgg
      361 atataataaa tcggcagtcc aaagccttaa tatgtattca acagagaaaa ttataacgga
      421 aacaatctct ataattaaga atatacggat ttttaatgcg gataaggaaa aggttgctgc
      481 gataatagtt acggtattaa ttaaaattaa tgtgacgata actaagtcga acattttact
      541 tagaatgctt gaattatcgg caggttgaat aattttaaaa attttatttt ttattgattt
      601 catcttatac acctactact ttaaatttga ttatttaatt ttatcatatt ggttagttaa
      661 ttgcaataaa tgtttacaga tgaattttta gttaatataa gttaaatgaa tatgcaagaa
      721 atattgacaa cagggtcgat ttttgatact attattatgt attactataa gtaaatttta
      781 gggaaggaca ggtgagtttt tatgactcct aaagtagcat taataatggg aagtgactct
      841 gattttgaaa aattaaagag ttgcgttgca aagcttaaag aatttaaaat tgaggtagat
      901 gtgaatgtaa tttctgcaca cagaacacct gacaaagcgg cagaatttgc caagaatgcc
      961 gagaaaaacg gtattgaggt tataatttgt gcggcaggta tggcggcaca tctcggtgga
     1021 gttatagcgg ctcacacaac agtacctgtt ataggtattc cgattaagtc aacacttgag
     1081 ggtatggacg cacttttggc aacagtccaa atgcctcctg gtatccctgt tgcgacggtc
     1141 ggtatagata acggcacgaa tgcggcgatt ttggctgcac aaatgcttgc tattaaatat
     1201 gattatttga aagacgctct aaaagacgca aaggttaaaa tggttgaggg cgtcgagaaa
     1261 aaggacgaaa aaattaaaaa agcagttgca gaaatgtaat ttgatattta agtgaaaaca
     1321 attttaaagg agataaaaat atgagtgaaa atgcgtacaa acaagcaggt gtagacgttg
     1381 aggcgggcta cgaatcagta agactaatca aggacgacgt taaaaagaca gcgattgagg
     1441 gcgtacttgg cggaatcggc ggcttcggcg gactttttga aatgccgaaa aactacgaaa
     1501 atcctgtact tgtttcgggt actgacggtg taggtacaaa aatcagagtt gctttcctta
     1561 tggacaagca cgatactatc ggtcaagact gcgttgcaat gtgtgtaaac gacattgctt
     1621 gttcgggtgc aaagccgttg ttcttccttg actatatcgc aatcggtaag aatgttccgg
     1681 aaaaggttgc acaaatcgtt aaaggcgttg cggacggctg tgttttgtcg ggttgcgcgc
     1741 ttgtaggcgg tgaaactgca gaacatccgg gaatgatgcc ggaagatgaa tatgacgttg
     1801 caggatttag cgttggtgtt gttgaaaaga gcaaaatcac tgacggtaaa aatgcaaaag
     1861 caggtaacgt gcttatcggt atcgcatcaa gcggtgttca ctcaaacggc tattcacttg
     1921 taagaaaagt tttcggtctt aatgacgata acgcaaagga aactcttaat acatattcag
     1981 acgaacttgg caagacaatc ggtgaggaac ttttgacacc gacaagaatt tacgttaaac
     2041 cgcttctaaa gcttattgac gaagtcggta ttaataccgt ttctcatatc acaggcggcg
     2101 gatttatcga aaatgttccg agaatgttgc ctgacggttt gaaagccgtt atcaaaaagg
     2161 gttcatggga ggttcttcct atatttaatc ttatgcagaa aaaggctgat attcctgaaa
     2221 gagatatgta caacacattc aatatgggta tcggtatgat agtagctgtt gatgcggata
     2281 aggctgaaaa ggctgttaag tgccttgagg acgcaggcga aaaagcttat attatcggtg
     2341 aacttgttga cggcgaaaaa ggtgtggaga tagtcggtaa taatgaggtt atctcgtaaa
     2401 aaatatgagg catctctcgg gtttaaaagg aacttgaagt ataaacatac atcagcgttc
     2461 cttttgctcc tgatatatac cacatctttt tcacgaggag gtttaattta tgaaaaaaat
     2521 aggtgttctt atttcgggcg gcggcacgaa tttacaggct ttgattgata aacaaggtac
     2581 tgttttgaaa agcggtaaga ttgtgtgtgt atgctcaaat aacagtaagg cgtacggtct
     2641 tgaacgtgca aaaaatgcag gtattgcgac taaagtaatc cgcaaaaatg ccgattgcgg
     2701 cggtgataac gacgaattta accgacaaat ttgtgcgtat atgaaagaaa tggacgttga
     2761 tgtgattgta cttgcaggat ttttggcgat tttgggcggt gacttgctta aagaatacgc
     2821 aaataaaatt gtcaatatcc acccgtcact tataccgtca ttttgcggtg acggctttta
     2881 cggattgaaa gtacatcagg cggcacttga ttacggtgtt aaagtgaccg gagcaacggc
     2941 acatctcgta aatgaaatca cagacggcgg tagaatttta ttacaaaaag cggtttatat
     3001 tgaggatggc gatacgcctg aaattttgca aaaacgtgtt atggaacagg cagaatggat
     3061 tattttgcct caagccgttg aattggtttg tgccgatatg gtgtgatgac caccactccg
     3121 tcacttcgtg acacctctcc ttaaggagag gcattatatg gctctccctt gaggggagct
     3181 gtcgactttg tcgactgagg ggtggcttta tggatacaaa gttaaaagga gaagtataat
     3241 gaaaaaaatt gatatatatg aagaactaaa aagcaaagac tatccgggtc gtggtattgt
     3301 aatcggaaaa agtgctgacg gtaaaagtgc cgttacggca tattttataa tgggcagaag
     3361 tgtaaacagc cgtaaccgtg tatttattga ggacggcgac ggtattcgta cacaagcgtt
     3421 tgacccgtca aagcttgaag acccgcatct tatcatatat gcacctgttc gcgtgcttgg
     3481 tgacaagact attgttacaa acggtgacca aacagatacg atttacgaac atatggaaaa
     3541 cggtcagaca tttgaacagt cattaagaac acgtgaattt gaggacgatg accctaactt
     3601 tacaccgaga atatcgggta ttattgaacc taacaagggc ggatttgatt attcaatgtc
     3661 aatcctaaag agtgctgacg gcaatccgca gtcttgtcaa agatatactt tttcgtataa
     3721 caatccgctt gacggcgagg gtcactttat ccatacatat atgggtgacg gcagtccgct
     3781 tccgtcattt gagggcgagc ctaaaaaggt tgcaattcca aacgatatta atgaattcac
     3841 agacggtctt tggaattcac ttaatgcgga taataaagtt tcgttgttcg taagatttat
     3901 cgacattgca acaggcgaaa caaaaacaaa gattgtaaac aaaaacaaat aatatttaca
     3961 taaataaaaa cgaaaggacg ataaaataat gaatgaaatg caattaaagt acggctgtaa
     4021 ccctaatcaa aagccgtcaa gagtgtttat ggagaatgga gatttaccgt ttgaagtaat
     4081 ttgcggaaga cctggttata ttaacctttt ggacgcgctt aacggctggc agcttgtaaa
     4141 ggaattgaag aaagcaacag gacttcctgc cgcaacttct tttaaacacg tttctcctgc
     4201 cggtgcggct gttggacttc cgttgtctga cacattgaag aagatttatt tcgttgatga
     4261 cgttgaactt actccgcttg ccaacgcata tgcacgcgca agaggtgctg acagaatgtc
     4321 gtcattcggt gattttatcg ctctttcgga tgtgtgtgat aaggctaccg cacttcttat
     4381 aaagaaagaa gtttcagacg gtgttattgc tccgggttat acagacgagg cacttgaaat
     4441 tttaaaagca aagaaaaaag gcaattacaa catcataaag attgatgaaa attataagcc
     4501 tgccgaaatt gaaagaaagc aagttttcgg tgtcacattt gagcaaggca gaaatgaact
     4561 ggatattaat gacgaattgc ttggaaatat agtaacgaat aacaaggaac tttcagacga
     4621 ggcaaagaga gatctaaaga ttgcgcttat aacattgaag tatacacagt caaacagtgt
     4681 ttgctacgtt aaggacggtc aggcaatcgg tatcggtgca ggtcagcagt caagaataca
     4741 ttgtacacgt cttgcaggca ataaggctga tatatggtgg ctgagacaag caccgaaagt
     4801 gcttggtttg cagtttgttg acggaattaa gagagccgac agagataatg cgattgatgt
     4861 gtatatttca gatgaatata tggatgtact tgcagacggc gtatgggaaa agacatttaa
     4921 agtaaagccg gaagtgttca caaaggaaga acaaagagca tggcttgata agaatacaga
     4981 tgttgcactt ggttcagacg cattcttccc gttcggtgac aatattgaaa gagccaaaaa
     5041 gagcggtgtt acagtaattg cacaacccgg cggttcaatt cgtgacgata atgttattga
     5101 aacgtgcaac aagtacaata tgtgtatgag tttcacaggt ataagattgt tccatcatta
     5161 atcccaaaga cgcataatca gttccatatg cacttcaaag caatggggcg gtacacgcgt
     5221 acagcaccca ttgcatttcg gcacatctga aactgattct gcgactttta ctggttggta
     5281 tatttcgtat taaacggctc ggggcggtac ctatgtacag caccgctcgc cgtttcaaac
     5341 gaactgcaca caattctgcg attttaattg gtgagtattt taattaaagg gagaacgaat
     5401 tatgaaaata ttagtagtag gcggcggcgg acgtgagcat gctattgttt ggaaaatagc
     5461 gcagtcgcca aaggtagata aaatttattg tgcaccgggt aacggcggta ttgcggaact
     5521 tgcggagtgt gttgacatcg ccgcaacgga tattgaaaat atggttaagt ttgcaaagga
     5581 caatgcgatt gatcttgtta tggttgcacc tgacgctccg cttgtacttg gtatggttga
     5641 cgcaatggaa aaagagggta ttcgtgcatt cggacctcgt gcaaatgccg ctataataga
     5701 gggaagtaag gctttttcaa aggaacttat gaagaagtac aatattccta ccgcaaatta
     5761 tgaagtgttc acagacagta cgtctgcgat
//
LOCUS       DAWACS010000040         5695 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1572_length_5695_cov_2.622518, whole genome shotgun sequence.
ACCESSION   DAWACS010000040 DAWACS010000000
VERSION     DAWACS010000040.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1572_length_5695_cov_2.622518"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2278..2287
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1795 a          941 c         1410 g         1539 t
ORIGIN      
        1 tatatcagaa acataatcaa gaaagaagaa gaacgctttg acgctacaat cgacaacggt
       61 cttaacgtac ttaacggata tattgaggca gctcaaaaag agggcagaaa agagcttacc
      121 ggtaaagagg catttaaact tcacgacaca tatggttttc ctgttgacct tacaatagaa
      181 atggctgaag aaaaaggtct tgaagttgat gttgacggat ttaacaaagc tatgcaggaa
      241 caaaaggata ctgcaagaga tgctcgtgca gaaggttcaa gctgggacgg caacgaaaca
      301 tttgaatttg aaaatgcaga gccgactgta tttgtcggtt acgatacact tgaaacagac
      361 gcaaaagttg tcggcattgt tgttgaggac gagggcgttt gtgatacaat aatcgaaaat
      421 caaaagggat ttatcgttac agacaaaact cctttctatg ctgaaatggg cggtcaggta
      481 ggtgacatcg gtactatcac aataaacggt aaggctgcaa atgttgttaa tacaacaaag
      541 acagaggacg gttattatct tcacgaaaca gaagttcagc ttacaccgat taaggttggc
      601 gatacagtta caatgtctgt tgacaaggtt cacagaacag acgtttgccg taaccatact
      661 gcaactcata ttcttgacaa ggcacttcgt gatgtacttg gttcacacgt tgcacaagca
      721 ggttcgcttg ttgaaagtga cagacttcgt tttgacttct ctcactttga ggctatgaca
      781 actgaacaga tcaaacaggc tgaagatatt gttaatgaga aaattcttga aagcattgac
      841 gtgactgtac aggaattgcc gattgaagaa gctaaaaagc ttggcgctat tgcattgttc
      901 ggagaaaagt acggtgatgt tgtaagagtt gtttctgtcg gcgattacag tgttgaattc
      961 tgtggcggta ctcaccttac aaatactgct caatgcggtt tgtttaagat tatttctgaa
     1021 agcggtgttg cggcaggtgt aagacgtatt gaggctgtga caggtaaggg tgtgcttgaa
     1081 tatatcaata acagtgacag acttatcgaa aagactgcgg ctgcgttgaa gattaatcaa
     1141 attaatgaaa ttgatcacaa ggctgaaagc gttatggcac agtgccgtga gcttgaaaag
     1201 gctgttgatt cgtttaagga aaaaatggct gccgcaaagg caaacaacat tatgacaggt
     1261 attaagcata tcggcgaaat cagcttgatt accgcacagg ttgacggtat gggtgctgat
     1321 gaaatgaagt ctatggctga caagattaag gcggaagttc cgaacagtgt tgctgttatg
     1381 ggtgctgaaa ctgacggtaa gattacgttt gttgctatgg cgtcaaagga ggctgttaag
     1441 ctgggcgttc attgcggtaa gattattaag gatattacgg ctgttgcagg cggccgcggc
     1501 ggcggtaagc ctgatatggc gcaaggcggc ggcagtgacg cgagcaagat tgatgacgcg
     1561 ttggctaagg ttgacgaaat cgttgcggaa cagattaagt aattgagaat agcgtaaacg
     1621 gtttggacta ttgtattgta ggggcgacca ttggtcgccc gcaatgttga ggtattaaca
     1681 gggcggccga tgaccgcccg tacgaaagat ataatagata agagagaaaa gataatagag
     1741 aatagatatg agtcaccact ccggcacttt gtgccaccta tattaaggag aggcaagaag
     1801 gctcccatag aggggagctg tcggcagagc cgacttaggg gtggtgtgaa atacaaagga
     1861 gtgaataaaa tggattgttt attctgcaaa ataattgacg gagaaattcc gtcaaataag
     1921 gtatatgaag atgatatgat gttagcattc cgtgacatca atccgcaagc accggagcac
     1981 attgttattg tgccgaagtg tcacattgca agtgcgaatg aaattaatgc ggaaaacgta
     2041 aagtatgttg cggctatatg ggaaaagata ccgcagattg cgtctgaatt gggatttgcc
     2101 gaaaacggtt acagagttgt aaacaactgt ggcgaggacg gcggtcagac tgttccgcat
     2161 ttacattttc accttatggg cggtaagaag tttacggacc tttaataaga aaattaaatc
     2221 agtcgggaaa ataccgacac gatagcagag gtcgagaaat tagttcggat tttgtgannn
     2281 nnnnnnnttc cggacaattt gtcgacaatc tgactgccgt tttaaacggc agtttttttt
     2341 gtcaaaaaac ggtttacttt tgtatatctt tattatataa taatataata ggattagttt
     2401 aattaggagg aaattaagaa tgggaaaact atttggaacg gacggtataa gaggtatcgc
     2461 caacaaagac cttactgccg aattggcttt taaaacaggt caaagcggtg catatgtgct
     2521 tacaaaacat tcttcaaaaa gaccgagaat acttattggt aaagatacaa gaaaatcagg
     2581 tgatatgctt gaggcggctc ttactgccgg actttgttcg atgggcgcaa aggttattcc
     2641 tttgggtgta gttcctacac cggctgttgc gtatcttaca agatactaca aggctgatgc
     2701 aggtgttgtt atctctgcat ctcacaatcc atgcgaatac aacggtataa agttctttaa
     2761 cagtgacgga tacaagctaa gtgacggaat tgaaaacgaa attgaaagct atatacttgg
     2821 tgaaaaggct atcgaagaat tgcctactca cggcaaagtc ggttatgtaa gtgctaacca
     2881 caacgctgtt gaggattatg ttgcgtttgc tgtttctacg attgactgcc gtcttgacgg
     2941 tatgaaaatc gctgttgact gtgcaaacgg tgcaagttat gaaaccgctt tcaaagctct
     3001 taacaagctt ggtgcaaatg ttgaggctat acacaacact cctgacggtg taaacatcaa
     3061 tcatatgtgc ggttctactc atatggaaag tttacaggct tacgttactt caatcggtgc
     3121 ggacatcggt atagcttttg acggtgacgc ggacagaatg ttggctgtgg acgaaaacgg
     3181 caagcttatt gacggtgacc agattatggc ggcttgtgca aagtttatgc gtgataacgg
     3241 tacattgaag caaaatactc ttgttgcaac ggttatgagt aatcttggct tatttatggc
     3301 aggtgaaaaa atcggtatca acattccgcg aacaaaagta ggtgacagat atgtacttga
     3361 agaaatgctt gcaaagggtt acaatatcgg cggtgaacaa tccggtcaca tcattttcct
     3421 tgaccacaac acaacaggtg acggtcttgt aagtgctttg caattcttat cggtacttaa
     3481 aaagacaggt atgaaagcgt ctgaggcggc aagcattgta acggttatgc cgcaagtgct
     3541 tagaaatgcc gttgtaaaac ttgaaaataa aaacagttat atggaaaatg aagaaatagc
     3601 tgcggcttgc aaggctcttg aagatgaatt tgccggcgag ggcagagtcc ttatcagacc
     3661 ttcgggtact gaaccgcttg ttcgtgttat gattgagggt aaggacgaag aatacatcac
     3721 aaagaaagct gaggaacttg cagaacttat tgaaaaaatc ttgggataaa agtggttgta
     3781 agaaatatgg ataaaataat ttcactaatg ataactgctg ttatgactgt tacgtcgtgc
     3841 agttttaaag gtgaaaatcc gcttgacggc aaaagaattg cgtttattgg tgacagtatc
     3901 agttacggca ctaattggca aggcggttac ggcaagttga ttggggaaca atataatatg
     3961 aatgttacaa atgtcagcaa gggcggtgct actcttgctg acaatgtgca ttggtcggaa
     4021 aattcagacg gttacagacc gtatattacg gatatgctcg ataaccttga cggcgattat
     4081 gaatatatca ttgccgaggg cggtttgaat gatttttggg gccattcgga acttggagaa
     4141 attacagacg gtttcagcgg tgattttgat gaaaacacaa tgaccggcgg tatggaaaaa
     4201 atgttttttg aaattagaaa tgattttcct aactcgaaag tagggtttgt tattacacac
     4261 gatccgttta catatgacgc cgaaggtaat tttgaatcgt attatgagcg tataaaagaa
     4321 gtatgcgaca agtgggatgt ttcgtatctt gatttgtatg caaaaaataa ttccgataca
     4381 ggtgtaaatg tgcgtgacga tgatatgcgc aagttgtatt ttggaacaga aagtaagcca
     4441 gacggtgacg gcactcatcc gaacgaactc ggttatcagg ctatttatgt tgagccgatg
     4501 ataccttggc tgaaatcttt ataaaatgga taggtgaatg atgaaggatt taattataaa
     4561 cggaagcaga cagcttgata taccgcttga tgatgtgcaa gttgataaac ttatggaata
     4621 tgcgcgtctt ttggttgagt ggaacgaaaa gattaatctt actgcgataa cagatgatga
     4681 gggcattgta acaaaacatt ttcttgactc gctgaccgca ataaagactg gatatgtcaa
     4741 gggaaaagtg attgacgtcg gaacaggtgc gggtttcccc ggacttgtgc tgaaaatcgc
     4801 aaagcctgaa attaagctga ctttgcttga cagtcttaac aagcgaatta actttttaaa
     4861 ggacgtttgt gaaaaggttg atataaatga tgtagaattt gtccattcgc gtgcggagga
     4921 cggcggtaaa aacagagatt acagaagtaa gttcgatacc gttgtgtcgc gtgcggtggc
     4981 taatttgact gtgctttcgg agtggtgtat tccgtttttg aaacaaggcg gttacttcct
     5041 tgctttgaaa ggtccgttgg cagagcagga agtaaaggac gctaaacgtg ccataaaaat
     5101 actcggcgga cagattgagg acattgttga ggcggaaatt ccgttcaccg aattgaaaca
     5161 caagataata gttataaaaa aagtaggaca aactccgctt aaatatccga gaaaaccggg
     5221 tattgcaaca aaaactccta tcggaacttg ttataatttg gtgaaataaa aaaataaaat
     5281 tgaatattgt cgaattataa ttgcaaaaag tacttgcaat atggaaatat tatgctataa
     5341 tagtaaatgt aaggcagata gtatcaatac ggtacatttc taacaaggtt tccccaaggc
     5401 cttgttctac ccccaaaatg tcttacacct tccaccaccg agggtaaaca gcgataatgt
     5461 tgttacatca agtgtaaaca tcaatgccga acattatcgc tggttactct ttttaatttc
     5521 agtgaaatta aaaagagcat gattaaaatg tacgtgcgag aatttcttcg cgaggctcat
     5581 aaaacgcaca tggacaaaca aagtgctgat cttccgtcac cacgctgata tgcaatgtga
     5641 gttgaagtga gaaagaggtg acgaaaggca cgttgttaga cttttaattt caaaa
//
LOCUS       DAWACS010000041         5477 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1663_length_5477_cov_2.173552, whole genome shotgun sequence.
ACCESSION   DAWACS010000041 DAWACS010000000
VERSION     DAWACS010000041.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5477)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1663_length_5477_cov_2.173552"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4052..4061
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5123..5132
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1512 a         1274 c          937 g         1734 t
ORIGIN      
        1 cggtttacca agattttcac gtctgtgagc agttacaata attactctct tattatcaaa
       61 agcaaggttg ttaaggcact catccttgaa cttataatca tcacggacag ttgttttaag
      121 cgcgtcaata accgtattac cggttatata aataatatcg tccgaaacat tttctttaag
      181 taaattcttt ctgttttgct ctgtcggtga gaaattaaga tcggcaattc tgcctgtaag
      241 ctgtctgttc atttcttcag ggaacggaga atatttatcg tatgttctca gacctgcctc
      301 aacgtgtccg accttgactt gattataaaa agccgcaagt gccgcaacga aacttgttga
      361 tgtgtcgccg tgtacaagca caatatcagg ctgttccttt ttaataactt cgtcaagacc
      421 ttccaaaact cttgttgtta ttccgataag actctgtctt gtcttcataa tatcaaggtc
      481 ataatcaggt gttatgccga atatttcaag aacttggtct agcatttgtc tgtgctgtgc
      541 cgttacacat acgattgatt caatattgtc atattttttc agttcaagtg caagcggagc
      601 cattttgatt gcctcaggtc ttgtaccgaa aactgtcata acctttaact ttttcacttt
      661 tctctatcct ttcctactta tcctcattac cttttttatc ggaaacttct tcaacgtcaa
      721 tttcattatc atcagtcaaa tttctgttat cctgatgttg atgtacataa ctgttctttc
      781 caagcatact tccaatcaaa agaagtataa gcacgcatat tacaagcaac aatgctctga
      841 gtacgccgct ttcagccaaa agtacagccg ttattccgag cacaccgctt attgcgtaca
      901 atataaatac ggtttgtttc tgggaaaatc ccatatccac aagtctgtgg tgcaagtgtc
      961 ctctgtccgc agtcatcgga ctttgtccgc gcaatatacg tctaatcatt gcaaacaatg
     1021 cgtcaaacaa cggcaatccc aaaatcaaaa gcggtactgc aaacgatatt accgcataac
     1081 tcttgaacac accttgtata gacaatgttg caagcataaa tcccaagaac gtcgagcctg
     1141 tatcacccat aaatattttt gccggattaa agttaaacgg caaaaatccg aaacacgaac
     1201 ccataagtgc aatacccaaa attgcaatcg aatattcacc cactcttatc gatataaaca
     1261 caagcgatat tgacattatc gccgacacgc ctgccgccaa tccgtcaaga ccgtcaataa
     1321 agtttaccgc atttgttatg aatacaatcc atataacagt aagcgttacc gaaagccatt
     1381 cgggaagtac ccaatacgga ttgtcgctta ggaaatttgg gtttgtgaac acatcgattt
     1441 ttatatcgcc tgcaaacaca accacaagtg cggcaataat ctgaattaca aatttcagct
     1501 tagcgggcag gtttttgcag tcgtctataa cgcccatgac ggcaataatc gccgcacctg
     1561 cgagcgttcc gtaaagctgc cgtgacggct gtgcaaagca caatgtcgcc accgtaaatc
     1621 cgaaaataat tgccaaaccg cctattcgcg gtgtaggttt tttgtgcatt cttctgttgt
     1681 ctttcggtat gtctatcgca ccgattttaa aagcaaatcg tcttaccaaa ggagtcgttg
     1741 caaacgtaaa tgcgaacgat acgatgaatg caaatatcat aaataaaact tgtcgagtta
     1801 acatattacc ctccattaca gcacaaagcc gactttctta tacacctttt tcaatgttct
     1861 ctgtgaaatt ctctttgcac tgtcagcacc tctttgacat atatccataa ggtactgctt
     1921 atcggcaata agtttatcat attcatttct gatcgggcgg atacattcaa caaccgcctc
     1981 cgcaacgtca ttcttaaagt caccgtaacc cttaccgctg taatccttta cgattgactc
     2041 aatattcctg cctgttacgg ctgacataat gctcaaaagg ttgttaattc cggcttttgt
     2101 cggatcgttt tcacgatatt caataacacc ctcactgtcc gttaccgcac tctttatctt
     2161 cttggcgata acattcatat cgtccatcat tgaaatatat cccttagggt taggatcgga
     2221 ctttgacatc ttcttttcag gctcttgcaa gctcattatc tttgcacccg actttgaaag
     2281 cataccttcg ggaattttga atacatcacc gtaaagcgag ttaaatctct gtgcaatgtc
     2341 acggcaaatt tcaatatgct gcatttgatc cttacctaca ggcacatagt ctgtttggta
     2401 aagcaaaata tctgccgcca taagtacagg atatgtaaac aatcccgcat tgatgttttc
     2461 agcatgacgt gcagacttat ccttaaactg cgtcattctc tgcaattcac ccatatatgt
     2521 gtaacagtta agtacccagc caagctctgc gtgtgcaggg ttatgcgact gaataaacaa
     2581 aattgtttct tccggattaa ttccgcaagc gatgtacaaa gcaagcactt caagagttct
     2641 tcttcttata tcagcaggag tttgacgaac tgtaattgta tgcaagtcca tcattgagta
     2701 tatacagtta taatctttct gtatgtcaac ccagtttttt atcgctccca aatagtttcc
     2761 gagtgtaaga tttcccgacg gttgtacgcc tgaaaatatg attttcttat cttccattat
     2821 tatcttcctt tcacagatta aagcatttct gtcaagactt ctcccaaacg tcttacacct
     2881 tcttctatat tttcaatagt agccgatgaa tagttaagtc tgaacatatt tgacggagca
     2941 tatatatccg ttgcaaaatt tgaaccgggt acgatagcga ctttcttttc aaggcactta
     3001 tcgacaatcg gtgtaatatc ttcgatagta gggcattcgc accataggaa tataccgccc
     3061 tcaggcacta cccacttaac tttaccctgt gggaaatatt tttccattgc actgaccatt
     3121 gcggcacatt tctttccgta aagctctctg ttctttgcaa tgtacttatc tatatcgtac
     3181 ttcttcataa attccacaca cataagctgt gtaatcatcg gtgtatgtac gtcatttacc
     3241 tgctttagca tttcaatctt ttccgcaagc ggtgcaggtg ctacaatgta gccaagacgc
     3301 atacccggtg aaagaatttt tgagaatgag cctgcataaa caactctgtc ctctgtatca
     3361 agcgacttga ttgtcggcac gtcctcacct gcaaaacgca agtcaccgta aggattgtcc
     3421 tcaaggatta gcacgtcata ctttgatgcg atttcaagca tacgctttct cttttcaagc
     3481 gacattgtac agcctgtcgg attttggaat gtaggaattg tgtaaatcat ctttacaccg
     3541 tccgtattct tcagagtttc ttcaagctcg tccatattca taccgtcagc ctcaacagta
     3601 acgccgacaa gttcagcctc atatgttctg aacgcattaa gacttcctat aaagcttgga
     3661 ctttcaacga taaccttgtc acccttattt aaaacagcct ttgcagtaag gtcaatacct
     3721 tgatttgcac ctgtcataat aagaatttca tcattttcac cgactgaatt aactttttcc
     3781 gcacgctttc ttgcacagtc tttcattttc ggatagccgt ctgttgtacc gtactgcaaa
     3841 gcaagagtag ggtttgtcat aagaatttta cccgcaattt ttgaaagttc gtcacccggg
     3901 aacaattcag acgcagggtt tcctcctgca agtgagataa tctcaggatc agccatacgc
     3961 ttgaacattt cacgaatggc actgcctttc atcgtcttaa ctctgtcagc cataaatttt
     4021 gtatattcca tttttcaaaa cttctttcta tnnnnnnnnn ntttcagatg ttgaatcttt
     4081 atcaacgcaa aaatatccca atctaaggaa ttggaactta tctcctacac ttagatttgt
     4141 caaattgctt tcaagttttg catttttaag aactatcata gaatctttgt taagattgtc
     4201 tgtaaagtca tttacgcctg tttcgtcaga cggattttca accgtaaaca atctgtcgta
     4261 aagacgtact tccgcgtcta ttgcgtgtgc ggcacttaca aagtgaattg taccctttac
     4321 cttacgtccg tcaggtgagt caccgccgcg ggtttcggga tcgtatgtgc agtgtacagc
     4381 aataatattg ccgtcctcgt ccttttcaca gcttgtgcaa gtaacgtaat acgcactttt
     4441 tagtcttact tctctgccgg gtgaaagtct gaaatacttc ttcggagcgt cctccataaa
     4501 gtcgtcttct tctatataaa gttcacgcga aaattctact gttctcttac ccatttcagg
     4561 attttcagga ttaacttcaa cttcaatctg ttcaacctta tcttcaggat aattgtcgat
     4621 tataagttta atcggtctta aaaccgccat tgctctcggt gctttcttat tcaaatcttc
     4681 tcttatacag gcctcaagca aagcaaagtc aacaacgctg tttgcttttg aaacaccgat
     4741 tctttcgcag aaatcacgaa tagcctcagg tgtgtaaccg cgtctgcgca taccgcaaag
     4801 tgttgacatt ctcggatcgt cccaacccga cacaatattt tcacgaacaa gtttcagaca
     4861 acgtctttta cttgtaagcg tatagttaag attcattctt gcgaactcaa tctgtcttga
     4921 cggactttca aagccgactt cttttaatac ccaatcataa agcggtctgt gatcctcaaa
     4981 ttcaagtgag caaagcgaat gagtaacacc ctcaacagta tctgaaatag gatgtgcaaa
     5041 gtcatacata ggataaacta cccatttatc acctgttctg tgatgatgtg cacgaagtat
     5101 tctgtaaata atcgggtcac gcnnnnnnnn nntatttctt ccggtgacaa atcgtcaaca
     5161 tatgccttac cctttttgat aagttttaca gccgccgcat aaatatcgtc aaaataatca
     5221 gacgcataaa gcaccttgtc ccacttaaag ccaagccact ttatatcctc cattattgcg
     5281 tcaacgtatt ctgtatcttc ctttgtaggg ttagtatcgt caagacgcaa gttacatttt
     5341 ccgttatact tttcagccat accgaaatct atgcttatag cctttgcgtg tccgatatga
     5401 agataaccgt taggttcggg aggaaaacgc gtttgtacat ggtcgtacac tttttcctca
     5461 aggtctttgt ctattgc
//
LOCUS       DAWACS010000042         5476 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1664_length_5476_cov_2.218779, whole genome shotgun sequence.
ACCESSION   DAWACS010000042 DAWACS010000000
VERSION     DAWACS010000042.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5476)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5476)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5476
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1664_length_5476_cov_2.218779"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    938..947
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2860..2869
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3126..3135
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1817 a          853 c         1329 g         1447 t
ORIGIN      
        1 ggacgcaatt gttaagggac tcagagcggt aactgacttt gaatatgaat ttcaaatggc
       61 acttctgaat aaggctctta atcctgaata cgaaactatg tttatgatga cgaatacgaa
      121 gtattcatat atcagttcga gcatggttaa ggagcttgcg ggatttcatg gagatttgac
      181 aggtcttgta ccttgtgaaa taatagaaac aatcgaagaa aaatataagt agggatgaaa
      241 ggattgaacg aaatggatat ggatatggat atcatggaaa ttgtcgatat gatggaggag
      301 gctattgaaa aagcatcggc tgtacctcta accggaaagg ttatgattga caagggtgaa
      361 cttttagact acattcagga aattcgtctt gtataccctg acgagctaaa ggaagcaaag
      421 tgggttaagg aagaaagaca aagaattttg taagaggctg aaaacagagc cgaggctatt
      481 cagaagaatg ccgaagaaac tcaaatgcag cttattgacg aacacgaaat tacaaaggct
      541 gcatatgaac aggctaatga gcttgttaat gccgctcagc agaagtctat cgaaattaag
      601 actgatacag accaatatgt tgatgacgtt cttaacgacg ttgaacacag attggacttg
      661 ctacttagaa aagttcgtga ggacagaggt tatttcaata ataactgatt aaataacgat
      721 taaatatttt cgagctggat taattttact gttcagctct atttcacttt ataaagatat
      781 taacatttta atgcacaata gagaatttaa gggaggatat gaatatgcta tcagatattg
      841 aaattgcaca aaatgcaaaa atgaagcata tccgtgatgt tgccgcatca atcggcatag
      901 cagaggacga tttggagttt tacggaaagt ataaggcnnn nnnnnnngta taaggctaag
      961 attgcctctg aacttttggg aaaggttaag gacaatgaaa acggaaagct gattcttgtt
     1021 acggcaataa atcctactcc ggcgggtgaa ggtaaaacaa cgacaacaat cggacttggc
     1081 gatgcactta ccaatatggg caaaaagtgc gtattggcac ttcgtgagcc gtcactcgga
     1141 cctgttatgg gaattaaagg cggtgctgca ggcggcggtt acagtcaggt tgtaccgatg
     1201 gaggatataa atcttcattt tacaggtgat atgcacgcta taactgcggc aaataacttg
     1261 ctttcggcaa tgattgacaa tcacattcat cagggcaacg cacttgatat tgatgttaca
     1321 aacattgttt ggaaacgtgt tgtcgatatg aacgacagag ctttacgtca cgttgttgtc
     1381 ggtatgggcg gtaaggtgaa cggtattccg cgtgaggacg gttttatgat taccgttgca
     1441 tcggaggtta tggcgatact ttgtcttgca aacgatttgg aggatttgaa aaaccgtttg
     1501 ggtgaaatta taatcggata taaccgcagc ggtgagcctg ttaaggcttc ggatttaaag
     1561 gcacagggtg caatggcggc acttttgaag gacgctataa agcctaacct tgttcagacg
     1621 cttgaatata caccttgctt tattcacggc ggtccgttcg caaacattgc acacggctgt
     1681 aacagtgtac aagctacaaa gcttgctatg aaactttcgg actatacaat aacggaagcc
     1741 ggcttcggtg ctgatttggg tgccgaaaag tttatggata ttaagtgccg tatggccgga
     1801 atcagccctg acgcggttgt tattgttgcg actgttcgtg cacttaaata caacggcgga
     1861 gttaaaaaga ccgaccttaa agaagaaaat gttgacgctt tgaaaaaggg tatcgtaaac
     1921 cttgaaaagc atattgaaaa tatgaagtca ttcggtgtac cggttgttgt ggcggtaaac
     1981 cgttttgata cggacagtga agaagaactt aacactgtca ttgaaagatg tcacgaatta
     2041 ggtgcagaat gtgcacttag cgaagtcttt gcaaaagggg gtgagggcgg attggacctt
     2101 gccgaaaagg ttatttcggc ggttgaaaac caaaaggctg actttaaacc ggcatacgat
     2161 accgatatgt caatcaaaga taagattaat gaaatcgtga caaaaattta cggcggtaag
     2221 ggtgttaatt acacaccgaa agcacaaaag cagattgtaa gacttgaaga actcggtctt
     2281 gataaaaagc ctgtatgtat ggcaaagacg cagtattcat tgtctgacaa cgcaaaactt
     2341 ttgggcagac ctgaaaattt tgaaattacg gtaagcaacg taagaatttc aaacggtgcg
     2401 ggatttatcg ttgttgaaac gggtaatata atggttatgc cgggacttcc gaaagtaccg
     2461 gcggctgata acatagacgt tgatgcagac ggaaagataa cagggttatt ttaattatgg
     2521 aatttgagtc aaacagttac gaagaaacac aaaaatttgc cgagaagttt tccaaaactt
     2581 taaaggcagg tgacgtactt tgtatgtacg gtgatttggg tgtcggcaaa accgcattcg
     2641 tgcagggact tgcaaaggga ctcggaattg acgagcctat cacaagtccg acttttacga
     2701 ttgtgaatga atacagcggc agacttcctt tgtatcattt tgatgtgtac cgcatagcag
     2761 acagtgatga aatgtatgaa atcggttatg aagaatatgt ttacggcgac ggtgtaagtg
     2821 ttatcgaatg gccgcagctt attgacgaca tacttcccgn nnnnnnnnna tgacgttact
     2881 atatcgaagg attacagccg tcacgataat tacagaaaaa tcgaaattca aaaaacggag
     2941 gcagaaaaat gagagttctt gcaatagata cctcgtccaa tgtcgctact gttgcggtta
     3001 tggaagatga gcttttgctt ggtgagtata ttttaaatca taaaaaaact cattcgcaga
     3061 agattatgac aatgatagaa cagattttgt cggagcttga attgaacgtt caagatattg
     3121 acatannnnn nnnnnatata tttgcggcgg ctatcggacc tggttcgttt acaggacttc
     3181 gtataggtgt tgcaactgtc aaagcacttg cacacgcaac gggcaaacct gtggtgagcg
     3241 ttggaacact tgaggcactt gcatataacg tgccgtatgc ggagcatata attgtgccga
     3301 taatggacgc aagacgtgac aatgtgttta cggcaagcta tatttgggac gagggcttta
     3361 aggaaatcgg agagcctgag gcaatcagca ttgatgaatg tcttgaaagc tgcggtgaat
     3421 ttcttgatac tatatttgtc ggtgacggtg ttaaggtgca tagggaatat attaaagaaa
     3481 aacttggtga caaggcgata tttccgcctg caaatgcatt aaattcaaga gcgtcatcgg
     3541 ttgcggcact tgcacttgat aaggcaaaac gcggtgaaac gcaatcgtat cttgaaatga
     3601 aaccgtatta tataagaaaa tctcaagccg aaagagagct tgaagaaaaa gaaaacaaag
     3661 aaaaaggaga aagcaaatga tagcaatagg cagtgaccac ggcggttttg aattaaaagg
     3721 acatataatc gaacatttaa aggaaaaggg tatcgaagtt aaagacttcg gaacatacag
     3781 tgaggcaagc gttgattatc ccgactgtgc aaagcctgta tgcaaggctg ttcttgacgg
     3841 tgaatgtgaa tgcggtatac ttatctgcgg tacaggtata ggaatatcaa tggcggcaaa
     3901 taagtttaag ggtataagag cggcactttg cggtgatgtg ttcagtgcga aaatgtcaaa
     3961 ggaacataat aacgcaaatg ttatttgtat gggcggtcgt gttttgggca gagagcttgc
     4021 aaatatgatt gttgacactt atcttgaagc ggaatttcaa ggcggcagac atgccgacag
     4081 aattaaaaaa attcacgaaa tagaaactat gtaataaaag acagattgtc tgcaaagccg
     4141 ttacggtttt gcagaccgtt cataaaagaa aaaacattca gtcaaatgct gtaattgggg
     4201 aagcgtaatg actgaatgtt tttttgctga taaataatta ccaactgaaa agagtataac
     4261 ataaatcaat atattagtca accgttattt taataaatta aactgtttct tgattataat
     4321 ttaaactatt agttgataaa atacaattag tattttatcg gagtggtaat atgaataaga
     4381 aattgtttga ggatatagga aagagagtta aaaagagaag aattgagctt gggtatacgc
     4441 aggagtattt ggctgaaaaa atggacgtca gtatacagat gatttcggga acggaatccg
     4501 gcaagaaagc tttgaaactt gaaaacttta taaaattttg tgaaattctc gaaactactc
     4561 ccgactattt gataaaagga cgtgatcccg catcgatatt gattgaagaa atggaacggt
     4621 tgacagatac acagattgaa agaataaatg cggtgataaa gcagtgtatc gagatgtgca
     4681 aagaacaaaa tgagcaatag gagatagttg caagcagaat attaaattca atttacatta
     4741 tatacaaaac tattgacata tatcgagtat aagatttata atttttacag agaataaggg
     4801 gtaaaaaggg gaaaattatg gagtttatac tgcctgacag cgttatgaat attttaagtc
     4861 gcctgaacac agacggcttt tctgcgtttg tggcaggcgg ggcggtgcgc gatacgatta
     4921 tgggcaaaac tccgcatgat tacgacattg caacgagtgc aagacctgaa gaagtgaaaa
     4981 aacttttccg ccgtacgatt gatacgggta taaagcacgg tacggttacg gttgttcaca
     5041 acaagacagg ttatgaagtt accacgttca gaactgacgg tgaatacagt gacggcagac
     5101 atccgcagag tgtatgtttt gtcgatgatg caagggaaga ttgtgcaaga cgcgatttta
     5161 ccataaatgc gatgatgtat ttgccgaaaa cgggtataat tgattatttc ggcggtcagc
     5221 atgatattga aaataagata atcagatgtg taggtaatcc cgaaaaacga tttaaagagg
     5281 acgcacttag aatgttaaga gcgatacgtt tcagtgcggt gctttcgttc agaattgagg
     5341 acgaaacatg gaaagcaata aaaaaatgtg cggtgcttat aaaaaaagtc agtcatgaaa
     5401 gaattttgga ggaagtgaat aaaatacttc tttcagagaa tcccgactat atccgaaaat
     5461 tgcacgagtg cggact
//
LOCUS       DAWACS010000043         5455 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1673_length_5455_cov_2.312222, whole genome shotgun sequence.
ACCESSION   DAWACS010000043 DAWACS010000000
VERSION     DAWACS010000043.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5455)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5455
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1673_length_5455_cov_2.312222"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1441..1450
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5070..5079
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1433 a         1255 c          933 g         1814 t
ORIGIN      
        1 acatcagaca gcttagcaag tttttcttca acctcagatt tagtaagcgg tgaagtcaca
       61 aacgctgact cattgtctat aatattatcg acaaattcgc actttccgaa tattttctga
      121 acattttctg cagaatcagt tgttcttaca aagtattcaa acttctttga atcaatatca
      181 gccataatat tttcagccgg cttttcccag aaaatcttct tacttctgtc gctgtgctta
      241 accgcgtcaa caacgtcggc aacaacagca cttgcagtag gaagtttacc tgcaccctta
      301 ccgtagaaca ttacatcgcc tacgcaatcg cctgtaacca taatggcatt gaatacaccg
      361 tcaacaccgg caagaggact gctgtttttt atcaccatag gtgatacgat tgagtaaacc
      421 ttgccgtttt cgtcaaactg tgcatatccg ataagtttta taactgaatc catagcagcg
      481 gcatatttaa catcgtcaag agtgatgttt gttataccat ctgtatcaat atcattgtaa
      541 tcaatcattt tgcccgacgc gagtgacgcc aaaatcgcaa tctttctgca agcgtcgtga
      601 ccttcaatat cggcggcagg atttctctct gcgaaaccgt tattttgtgc gtcttttagg
      661 gctgtttcaa aagtcttgcc ctttcttatc atctggtcaa gaatatagtt tgttgtaccg
      721 tttaaaatac cggcaatctt caatatattg ttagccgtta gacattgatg cataggtctt
      781 ataatcggaa taccgccgcc tacacttgcc tcaaagaagt agtttacatt tttccctcgg
      841 gcaatctcca aaagctctgt cccgtatgtg gcaacaagct ccttgttaga cgttacaacg
      901 ctctttcccg cttcaagaag actttttgta aattcatatg cagggtgaag tcctcccata
      961 acttccgcaa caacgcttac ttcgtcatcg ttaagtatgt cattgaattc ttttgtaaaa
     1021 agttcttttt cgggatggtc gtcaaaatcg cgaatgtcaa gaatgtactt tatatccaca
     1081 ctttcaccat tcgcactgcg gctgagcttg tcactgttgg tcttaattat gtcgtataca
     1141 ccacttccaa cagtaccgta gcccattatt gcaacctttg ccattcttat gcctccctta
     1201 tttttctttc attataatat gtattttatt atagaaatat ctataataaa atatattacc
     1261 atatcactgt gataaagtca atgttttcaa acgccaaata tgaccttttt aatcaaagtt
     1321 cttttgtgta aattaacact aacattttta tcgtctgtta tttttcttta tctctctgtt
     1381 aatcttcgca ttcattccgc tgccttttaa atacggcaga atactcatac atttgccgtc
     1441 nnnnnnnnnn gggtgattgt gcgtaaatac aacctgtgac gcgtcacttt taaccgcact
     1501 tctaagcacc ttcgcaacgt caagttcaac gctttcaaaa ctgccctcga aaagcatttc
     1561 ctgattaata attctgtttt tcggatcaag acaaatcata tagcaacatt cgcggctttt
     1621 gccgtcaaac agattgcaca aataattttc actcgtttca ctgtttaaaa caaacacgtc
     1681 cgtattatat ttttccgtat tgtaatactg tataaattca ccgagagaac atatgtaatc
     1741 ggcggttttt tcacctacac cgtctatgct cataagacgt tttttagaag tgtggaacac
     1801 attcacaaat ccaccgcact ctttaatcaa attatgtgct atatcattcg tatccgccac
     1861 cggaattacg ttgtagagaa gatactccaa aaccttatgt ttctgccacg ttttgaaacc
     1921 gcctttttca aaatcctcac gcattctttt tctgtgacct ttgtgattgt ttgtgtccat
     1981 tatttcaccg ccattacttc attctacggc ttattataca ctattttact ataatttgtc
     2041 tacaaatttc gacaaattat ttacacaaaa aacggagcat accgtttgga tatgctccgt
     2101 ctgaatattt aattacttgc cttcaaccaa tctcttagtg tcaatagcaa tcataagttc
     2161 ttcgtttgta gggataacaa gagttttaac tgttgcatcg tcagctgaaa tatcaactgt
     2221 acctcttgca gcgttcttgt cctcgttaat cttgataccc ataaagtcaa gaccttcaac
     2281 aactgccgat cttgtagccg catcattttc acctacgccg gcagtgaata caactgcgtc
     2341 aacaccaccc atagcggcag cataagcacc gatatctttc ttaacttggt atgagaacat
     2401 atcaagagca agctgtgctc tgtcatttcc ttctttagcg gcagcagaca aatctctgaa
     2461 gtcagatgat acgcccgaaa cacccgaaac tccgctttcc ttgttcataa gagcgtcaac
     2521 gtctttagct gacatacctt tttggttgat aagatatgta acgacagcag ggtccattga
     2581 acctgttctt gtacccatcg gcacaccgtc aagaggagtg aatcccattg atgtatctac
     2641 acacttaccg ccgtcaacag ctgtaattga tgagccgttg ccaaggtggc aagtaacaat
     2701 cttaaggtct tctgccggct tgcctaaaaa ttcagcagcc ttttgtgaaa catacttgtg
     2761 gcttgtaccg tggaaaccat actttctgat accgtagttt ttgtagtatt catatggcaa
     2821 agcatacata tatgcctttg gaggcattgt ctggtggaat gctgtatcaa atacgccaac
     2881 cataggagtg ttaggcataa tcttttggca agccttgata ccgataatgt tcggtgggtt
     2941 atgtagagga gcaagcggtg tacacttttc aagtgctttc ataactgctt ctgtaattac
     3001 gcatgaacct gcaaattctt caccgccgtg tacaactctg tgaccgactg caccgatttc
     3061 gtccattgac ttgataacac cgattttttc atcaacaagt gcgtcaataa ccatctgaat
     3121 tgcgtcaccg tgatccttca tcggcttttc aatcttagtc ttttcgttct ttgcaacatt
     3181 tgtgtgttga agatttgaac cttcaatacc gattctttcg caaagaccct ttgccataac
     3241 ttgttcgttg tccatatcta taagctggta cttcaatgat gaactacctg catttataac
     3301 taatattttc atattaaaat tcctttcatt ttttgattaa ttattttgag cctgtatagc
     3361 tgtaattgct acaacacctg caatatcctc tgcactgcag cctcttgaaa ggtcgtttac
     3421 aggctttgcg ataccttgaa gaataggtcc gtaagcttcg gccttagcaa gtctttgagt
     3481 aagtttgtaa ccgatgttac ccgcgtcaag gttagggaaa ataagtacat tcgcaccgcc
     3541 tgcaacgtct gatgaaggag ctttcgactt agcaacgctg tcaacgatag cggcgtcaag
     3601 ctgaagttca ccgtcaagct ttaattcagg agccttttcc tttgcaagct ttgtagcctc
     3661 ttgcattttg tcaacaaggg cactctttgc actgccgtat gtagaatatg aaagcattgc
     3721 aactctcggt tcgtcttgga taagctgttt gtatgatgca gctgatgaaa tagcgatttc
     3781 acttacttcg tcagcagtcg gatcttggtt aagaccacag tcaccgaaaa taaatgtacc
     3841 gttgtgaccg tattcgcagt taggaacatt cataataaag aatgcagata caagctttgt
     3901 acccggtgca gtctttagaa tttgaagtga cggacgaatt gtgtcggctg atgagtgaat
     3961 agcacccgat accataccat cagcatcgcc ttgtttaacc atcattaccg cataatatga
     4021 cggatctttg attgtttcgg cagcctgttc aggtgtaatg ccctttttct ttctcaattc
     4081 atagaatacg tttgcgtatt cttcgttctt ttcactgttt tcgctgtcaa caattttgat
     4141 accgtcaata tcaaggtttt cagcggcagc cattgccttt attttgtcag ggttacctac
     4201 aagaataatc tttgcatagc cctctttttt taccatttca gcagccttca aagttctgat
     4261 ttcttcgccc tctgcaagaa cgatagtttt gatgtccttt ttggctcttt cctttaattg
     4321 ttctatgaat gtactcatta cacaaaactc ctttttctct ttataatttt ttacctcttt
     4381 ttattatata cctttttttc gtctttttca agtaaatatc ttaaaaaatt gctttatttt
     4441 ggcttgtgaa ttttctaaca atttcactca gctatttacc cacgcaaaaa ctgcggaata
     4501 tcgccgtaac aatatcgtcc gacacggttt cgcccgtaat ttcaccaagt gcgtcaatgg
     4561 cgatattaat atcaatagat gcaatatcgg acggcatatt catatcaata gcctcaacaa
     4621 cccgtccgag tgcttcgcct gcttttataa gtgccgtctt gtgacgcata tttgttacaa
     4681 cggcactgtc ctctcttgca agctcgccga aattataaac cttgcggatt tcatcggcaa
     4741 gaatatcaag tcccataccc gttttcgcac tcacttcaat aacaacactg ccgtttgcct
     4801 ttgccttaac cgcctcaaca tatttgcttt gacccaggtc ggttttgttg ataagaacaa
     4861 ttcgtttttt gtttttggtc gcgtgcagaa cttccacgtc ctcatcaccg aaaaatccgc
     4921 tgccgtctat cataacgaca acaaggtcag ccttttcaat agatttaatc gacttttcaa
     4981 cgccgatttt ttcaaccgta tcatcggtat ttcttatacc tgccgtatcg ataagcataa
     5041 gaggtattcc gtcaaggttg atatattccn nnnnnnnnnc ctctataaca tcacgtgttg
     5101 ttcccgcaat gtcggtaaca atcgctcttt cctctcttgc aagcgaattt aaaagtgacg
     5161 atttaccaac gttcggtttg cctactataa ccgtctttat accgtcacgc aatattttac
     5221 cgctgtcggc ggttgacaaa agtttattca ctcggttttt gcagtcatgc gcaacatttc
     5281 ttatatcgtc agtcgtaata tcctccaaat cttcatccgg ataatctatc gtgacttgca
     5341 tttgtgcggc aaggtgaaca agctcatttc ttacattttc gatttctttt gaaagtgaac
     5401 cctcaagctg cgaaagtgcg tttcgctgtg caagttggtt tgttgaattt ataat
//
LOCUS       DAWACS010000044         5360 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1713_length_5360_cov_3.043921, whole genome shotgun sequence.
ACCESSION   DAWACS010000044 DAWACS010000000
VERSION     DAWACS010000044.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1713_length_5360_cov_3.043921"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1544 a         1077 c         1100 g         1639 t
ORIGIN      
        1 aattctataa ataaagagca tatggctgag atgcgtgcga ttaaagaaaa acttgaccca
       61 agcggcggaa gatttatggg ttgccgtaca ttgaatacag aggacgttat agaagaatcc
      121 gaatttgtcg gaactatgct taaccgccat gccgcaagat ttaccgccga gcatggaccg
      181 attatggaaa ctgaatattt gcgtgaagaa gcaccgagaa gaatttggga cgatttttcg
      241 ccgcctgatt ttgattataa gacaaagtgg ggcggaaagg gcggccgtaa gcaggtcggt
      301 attgattttt acgatatgac gtcggaggac ttggcacttg cttcggctaa aggctatggc
      361 gaatttttta atgacagaat aggcggtgcg tcgaaaaagg attattacag cggttgtgcg
      421 gctttgtgct ggacagattc ggcacaacac ggcagacagg cgtacagtga aaacgcaaga
      481 atgagtggca gagttgacgc ggtacgtaat aaaaagcaga gcttcgacgt gttccgcgtt
      541 atgcagtcac cgaaaagtgc ggttaaaatt ttcggtcacc ggaattatcc taaagctgac
      601 ggcgacaatt acaaatacaa tgtgaaaaag ttcaacggca catattggaa agaaacaggc
      661 gaaacggatt ttcgtgacgc atataataag acggtgtatg taatcggcag ttatgatatt
      721 gcgaaaattg ttttaaaagt caatggcaga gaagtcgggg tgtgtgataa accgatttac
      781 acatttgtgt tcccgtttcc gaatatcgac ataaccgaaa gcggtgagat aaccgcatac
      841 ggatatgact gtggcggcaa tcttgttgca agtgacacga ttaaaacagt cggtgaaccg
      901 tcacaaattc acttaacact tcacaccgat acaaacggac ttaaagctga cggcaatgac
      961 gttgcatacg ttgatattga agtaaccgat gaaaacggcg atatttgtcc gttgtgttat
     1021 gataaaattg actttgaatt cagcggtgac ggtgtattct tgggcggata taacagcggt
     1081 aaatttgatt tcggagataa gcaagagagt gttatacata aaaattacgt ctatgccgag
     1141 tgcggtacga acagagtgtt tattcgttcg acagaaaatg tcggaaagct aaagctaaaa
     1201 gcgaatatga acggcataac ggcggaaatc gaatttgaaa gcgttgaaac ccaaaatgat
     1261 actcttacgg aatgtacgtt tgacggaata tacgaagatt gcaaaaatga cgcagacaga
     1321 gcggaatttg aggcgataga acaaatcgat aacattaagt atgttcccga atccgagcct
     1381 tactgcaaaa tacttgtgaa cggtcaagag ccggatacaa ggggagtaag gagcctgaat
     1441 aaaaacggtc gtatatgggg tgcggtgatt tgtattcttg aacgtcttaa aaccgagtgg
     1501 agcgaacatt tcgactttga atataattcg gacgaaaaaa tacttacact gaaaagcggt
     1561 gaatatgtcg ttacggcaga gtgcggacgt actcatttac ttgtaaacgg aaatgaaaat
     1621 cttatggacg gtgagccgta tatttcggac agcggacaat tcgttatgga agtcagcgcg
     1681 atagcgtcat atattgacgg cgtaagtgcg tggtatgacg ataaagtaga agttttcaga
     1741 atagaaacca aacaataaaa gactgtcggc aaaaaatcgc cgacagtctt ttttaatcat
     1801 tatcattatt gttattcttc tttaattttc tgcggccgtc tataacgacc aaaagcagaa
     1861 atattacaat aagaacagtc aatacaatag cttttgcaga cataatttat tctccttatt
     1921 tacagaaaac gtgttttccg atagttttaa taataggtct tgtacgaatc catgaacttg
     1981 ttgctgtgga tgggttgtag taatatattg caccgcctgt cggatcccag ccgttcaaag
     2041 cgtcacgcgc ggcattatag caagatgatt gtatcgcttt gttaatctgt ccgtcatcaa
     2101 cggcggtaaa tgcaccgggt tgatatacaa ctcctgcgat tgtgttcgga aacgaggaat
     2161 gacgtacgcg gtttaaaaca accgcaccta ctgccacttg tccctcatag ctttcacctc
     2221 ttgcttcacc gtttataacc attgcaagaa gtgttgtatc attagaatat gaacttgatg
     2281 atgagcctgt cggcaaacct atttttgaaa gtgttgcggg acctgagata ccgtcggcgg
     2341 taagaccgtt cttacgctga aattctttca ccgcattggc ggtacgccaa ccgtatattc
     2401 cgtcaatact tccgctgtaa tatccccaag acttaagcct tgtttgaata tttgtgactt
     2461 cctgaccttg cgagcctatc tttgacaatg cgaaaacggt actttcgcaa aacacacaac
     2521 atattactgt tgacaataaa attaccgata ttcgtttaga tatatgtttc ataagaaatc
     2581 ctttctttct tgatgtaata tatatctatt tttaacggat attttgtttt taatacgcaa
     2641 ttataggtaa ttttatttga acgtgaattg caagcgtcaa agcacatatt tgctcagcac
     2701 cgttattcgc caattctctt gcacaagact gagcggtgtt tcctgtggtg taaatatcgt
     2761 caaaaagcaa tattttctta ccgtcacatt tttctgtgca tttgaatgca ctctgaacat
     2821 ttgtaacacg ttccgttcgt acaagtgaac tttgcttttt agttgctttt gtgcggataa
     2881 gcaaatctgt acgcataggt atttttaaat actccgcaac ttcttttgct ataagttcgg
     2941 attgattgta accgcgttcc tttaaacgtt tcttgtgcag aggaaccgga acgatataat
     3001 caaatgtatc ccatatatca tatgaatcaa gataattttt catcatatcg gcaagtaatt
     3061 ttgcataggc tttgcaattt gagaatttta aatcaagtat aactttacgc atactttttg
     3121 tgtattcaaa gggggacatt gtgaaagcga tatgttccgt accttgataa ggtgaggcgt
     3181 atgccgtttt gtcaagattt tcaaaacagt gacggcatat gccgatttta ccgtttataa
     3241 ggtgagcggt gtcgctctct tttcgttcgt cacaaaatac acaaatatac ttactcatat
     3301 taccactttt tcttcggaca tttgccgtcc gataatctcg ctcttacctg cataacacag
     3361 ccacatacac cgcacgttga gccgtacata aaatcatcgc agttttgaca aatatcaaat
     3421 ctgtttttat atacatcact gcttacaagc cgtacacttt tcattgaagt gacttgctca
     3481 accatttttt gaatatcgtc ttcggaaaga acggttttta atccgcaccg tttacatttt
     3541 gccattactt taatgttatt acacaagcac tcattttagg cagcttgaat gaaagtttac
     3601 cgttttcaag tgatacatca aactttttcg gctcaacggt gttcggtgcg tcaaatgtgt
     3661 tgcagtcgtt catatgctcc gatacaagaa tttctccgtc tgcaacagaa aattcactgt
     3721 cacgcaaatc aagctccata tcttcgtcac tgtcaagact tgtgttgcag attgaaacag
     3781 tcatttcacc gtttttcttt gaagcggaaa tgctgatttt agggaacttc aagccgttga
     3841 tttcctgttc ttcgcagtca tagtcaacgt caactctttc gccgtccata tgacctttca
     3901 tcattctgaa caagtgatat gtaggtgtaa gcaccatttt ttcaccgtcc gtaagaatca
     3961 ttgcctgaag tacgttcacc atctgtgcaa tgttagccat cttaacacgg tcgttgtgct
     4021 tgtggaaaat gtgaagtata agcataccgc ttataacgtc acgcatagta ttctgctgat
     4081 ataaaaatcc cggattagtt ccgatttcaa catcaaacca gttgccccat tcgtctacga
     4141 taaggtcgat tttcttttcg gggtcatatc tgtccatgat tgcagtatga acgtttataa
     4201 ctttgtccat cttcatagcg ttagccataa gtttatacca accgtcattg tcaaattcgg
     4261 ttgcactgcc tttgttttcc caagcacttt caaaagtata gttgtgcaga gagataccct
     4321 ctgtcatatg acggatattc tgcattaatg tttccatcca acgagtatcg tcaacgttcg
     4381 gaccgcctgc gattttgtag attggctcat cgccgtagtt tcttataaat gttgcatatc
     4441 tcttgtatag gtcggcgtaa tattcgggac gcatattacc gccgcaaccc cagttttcat
     4501 tgccgacacc gaagaatttt aacttccaag gcttttcgcg gccgttttta cggcgttcgt
     4561 ttgcaagcgg tgaatcaccg tcaaatgtca tatattcaac ccagtcacgc atttcctgta
     4621 cagtaccgct gccgacattt ccgcatatgt acggctctgt tccgagctgt tcgcaaagtt
     4681 caaaaaattc gtgtgtaccg aagtggttgt tttcgacaac accgccccaa tttgtattta
     4741 ccatataagg acgctgtgat ttttcgccga taccgtcacg ccagtgatag tcgtccgcaa
     4801 aacaaccgcc cggccaacgc aataccggta tttcaagctc ctttaaagcg tttacaacat
     4861 cacaacgcat accgttcttg ttaggtatgt ccgaattttt atcaacatat aatccttcat
     4921 atatacatct gccaagatgt tcggcaaatt gaccgtaaat atatttgctt atctttcctt
     4981 gatttcttgc tgtatttatt attgcttttg ccatttcatt tcctctcttt cacatacaaa
     5041 aaatatccgt tttgattata ctatgaccga attttaaatg caatatgtta aaaaaaatac
     5101 tctgatattt aatgtttttt tctgattttt aaagtacttt tatgaataaa cctttcataa
     5161 cgtcaagcga tttttgatgc aattcttcgt ctgccgcggc ggtaagagat gagtcgacaa
     5221 ttatttcgga attcggacaa gccgattttg ccataacggc atttgaaagt acgcatatgt
     5281 tgctcacaag tccgcaaagc tctattgtat cgaagtcttt gccttcaaga tactttgcca
     5341 tttcaagcga tgggaatgta
//
LOCUS       DAWACS010000045         5347 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1718_length_5347_cov_2.568405, whole genome shotgun sequence.
ACCESSION   DAWACS010000045 DAWACS010000000
VERSION     DAWACS010000045.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5347)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1718_length_5347_cov_2.568405"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1044..1053
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2006..2015
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3253..3262
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1697 a          895 c         1147 g         1578 t
ORIGIN      
        1 gctcctttag ggcaaatatt ttgcctgccg cccaaaaaac attgctgttg acatcaacat
       61 tgtcaagaat attttcaacg ctgttccaaa taacgtcaag acctgttttt ttataatatt
      121 gtgcacctat ggaatcggta accattttta tattaccgtt tttttcacgc catttcaatg
      181 cggaaagaat tgttgtgagt atttcaaaat cttcgacttc ataaggtgcg ttaaatctgt
      241 ttctgaaagg ctttgtccaa tttatatgta ttgcgtccat attgccacct atatgagatt
      301 aattccgtaa ccgtttatat atacacaccc actgcctgtt acaaagcttg tggcaaatga
      361 tgattgtgcc gaggtggtgt atgatgatga atagcttgtt atgtattcgt actcatattc
      421 aaattgatat gacgttatat aggaagaaac cgaaggtgaa tgttcgcata tctgtggtgc
      481 aaattgcggt acaaatccga tacaatttat gttgtgcttt ttcatatcgt cttttttgaa
      541 ttttgctttt gcggatattt cgcctattga tatgtatgtt acaccggcta tttgcggtaa
      601 tataaagtca ttatcgcaaa gcccgattga cgtacagcat aagtttaacg cattgtcaag
      661 ctcgtcctgc gtaaatgcga ttgtgtcaaa tgtcatacgt taaccccccc tatataaagt
      721 atattataat tatatcataa agatgataaa acggatagag ttatttttga aaaaaaccgt
      781 cctcaatcat tttatttatc tgtgcaccga acaggaatat gttcatacaa aaataaagcc
      841 aaagcattaa aaatacgatt gcggcaagtg aaccgtaaac gaatgaataa tttgaaaagt
      901 gttctatata tattgaatag aagaatgaga acaccatcca gccgaccgcc gaaaatgccg
      961 caccgggaag ctgtgcctta aagcgattgt cctttttggg cagaaacttg tagaacagtg
     1021 cgaatacaaa ggtaagataa atcnnnnnnn nnnatataag tatcttgccc cttaaaaaga
     1081 agtcgagcaa tgtcgagaaa aacggcgaat gtgaattgat aaagtattca agcttgcttc
     1141 cgaaaccgaa aaacacaatc gtcaagataa gtgccgcaat aaatgcaagc gtatataata
     1201 tagaaataag tctgccgata aagtaatttt tgctgcgtga acggtagact ttgttaagtc
     1261 ccgtatacag tgccataacg ccccttgacg caagccagag tgttgatacg gcggttacgg
     1321 aaatgacttg caccgagccg cttttggtaa ataaatcggt tattgtttcc gtaagaaacg
     1381 tgctgattgc cgcaggggca aatgaattta ttgtgtgcaa tattgagttt atgtctatat
     1441 taatgaacag tttgattatt gaaagtacaa gcatactgaa cggtataacc gaaattataa
     1501 taaaaaaaga tgcctgggcg gcgtatatgc tcaaatcatc accggtaatt tttaatataa
     1561 attttatgat ttttgaaaat atgtctttta tcgttttcat aaaactttct cctatcagtt
     1621 actatattat aatataacac atattgaatt tttagtctac taaatttaat taacatttgc
     1681 gttatattac cttgacagtg agtgagtatt agtatataat atccacataa aaggggagct
     1741 tttcggctga gaggaagttt gacttcgacc cattgaccgt atgcgggtaa tgccgtaacg
     1801 ggattttgga aacggctctc tttttgagag cattttttta tgctcaataa aaagaaagag
     1861 gagatatata tgaattatac aacacaaatg gatgccgcta aaaagggtat tataacacct
     1921 gagatggaaa tagtggctaa aaaagaaaat ataaccgcag aggaacttcg tgaaagagtt
     1981 gcaagaggca gtgttgcaat tcctgnnnnn nnnnntatga aagctgaggc tattatggac
     2041 cttagctgtt acggcaaaac tcacgaattc cgtcagaaac ttattgacga atgccctgct
     2101 atgatcggta cagtgccgat gtatgatgcg gttggttatc ttgataaaga gcttgcggat
     2161 attacggctg acgaattttt ggaggttata gaggctcacg ctaaagaggg cgttgacttt
     2221 atgacaattc atgcaggcat aaatcgcaga actgctcaaa tatttaaaga atcgggcggt
     2281 cgtcttacaa atatcgtttc aagaggcggt tcgttgattt ttgcttggat ggaaatgaca
     2341 ggcaatgaaa atccgtttta tgagtattat gacaaggtgc ttgacatact tgcaaaatat
     2401 gatgtaacaa tcagtcttgg tgacgcttgc cgtccgggtt ctacacatga ttcgacagac
     2461 gcgtcacaga ttacagagct tatcgagctt ggtattttga caaaacgtgc ttgggaaaag
     2521 aatgtgcaag ttatggttga aggtccggga catatgacga tagatgagat tgcggcaaat
     2581 atgaaacttg aaaaacgtct ttgccataat gcaccgttct acgtattagg tcctcttgta
     2641 accgatattg caccgggata tgaccacatc acttctgcaa taggcggtgc tattgcagcg
     2701 gcaaacggtg ctgatttcct atgctatgta acacctgcgg aacatttaag acttcctaat
     2761 gccgatgacg ttaaagaggg tattgtggct gctaaaatcg ccgctcatgc cgctgatatg
     2821 gctaagggtg tgccgggtgc aagagatatt gacaacaaga tgagtgacgc aagaagacgt
     2881 gtgtgctggg acgaaatgtt ccaatatgct attgaacctg aaaaaccaca gagatattat
     2941 aacgaattgc cgccggaaga aaaacatact tgctcaatgt gcggtaagat gtgcgccgca
     3001 agaactatga ataaaattct tgccggtgaa aaggttgatg ttaaataatt gaatgtgtac
     3061 atatttaaag cggataacta aggttgtccg ctttttacgt gggaaaatag gcattgcgga
     3121 gatgttgtga gcaatgtagg ggcgatcatt ggtcgcccca tacggaggga tgggagataa
     3181 aagatatgag agagccaccc ctcagtcaac tgcgttgaca gctcccctca atgggagcct
     3241 ttgggttagc ggnnnnnnnn nnggagaggg ggaccgcttg cggtggagtg gtggcatgga
     3301 gttatgtaac aaattcgacg ctgtatttat taaaaacgct gtttttgagg atgtttttat
     3361 gaatgaaaat ttattcaata aaaaatttat tataataaga agaaactcga acgaaaatga
     3421 gtaaaatatg gtgtgattta aaaaatattt taaagaattt ctcagaaatt ttaacaggaa
     3481 aaaccgcaca gtggtgcgga ttgtagcaat ttcttcacaa cttcttaaaa ttttgtaaca
     3541 aagttgtaat attatgtgca agttttaaca taaatcgttc aatatcttgc gtgggataaa
     3601 aaaatatgtc aatatattgt agatgtaaag ttttttatgt taatcgtaaa tgtaatatac
     3661 tgtaaaaaca atgaacaaat ttttgtaata tttcagctta attacgtgaa tatttcagct
     3721 gttttactgt aattttacaa gaacaataca aaaattttaa gaaatttaac atttttactt
     3781 gccaaaggct tgacacagct attttttagt gttacaatac agtcaacgtt caaaaagcaa
     3841 agactatgaa cgtaagacaa aatattaacg cggataatac aaacatagaa aaaactcata
     3901 gggtaaaggt ctttaaagta aaaaaggaga gattatatct tgaataagtt agcaaagaag
     3961 attatcttat ctgttatatc gatgtcagcg gttttaggaa cttctgcttt ggcggcagac
     4021 ctaagcggct gggctgtatc cgactaccaa caggctaatg aagccggcct tgtttcgtac
     4081 tcggtggttt caaacaacct aaaggataac ataactcgtg aagagttctg tgagcttgcg
     4141 gtaaacttat acgaaaagct tacaggtgaa gatatgatag aaccggaagt gtcacctttt
     4201 gaagatacag atagcatggc ggtagcccaa gcttactgtt atggaatggt ttccggtaca
     4261 ggtgataata catttacacc tgacagactt gtgacacgtg aagaaatggc aaaaatgctt
     4321 gtaagtacac ttactgcaag tgaagtcaat tttaaccttt cagacggtta tgacgacgct
     4381 gatgttgtca atgcatttga agattccgac gaagttagct cttgggctaa atcatcggta
     4441 attacaatgc ttaactactc attaatgagc ggtgttgacg atgaaaactt ctcgccgttg
     4501 ggttcaacaa caagagaaca agccatttca agtatcaatc gttcatacaa tacatttaag
     4561 agcgatgatt attcaatcga acttcctgaa attacacttc cggaagacgg tgcagaaatt
     4621 gaagaaggca actttacagt gtcttggaca cctgtaagta atgcacaagc atatcacgtt
     4681 attataaagg acgcaaacgc aagttcggtt cttttgtcag acgtatatga cggcgaaagc
     4741 attgaagtca gcgaaggttc gcttagagcc ggtaatgatt actcaataac aatcggtgct
     4801 gttattgcag acggctcaga agtttacagc ctgcctgttg actttaaata tgtggcaaag
     4861 ccgataatac caaacagtga atatattcag gaaaatcctg ccgcacaggg cttgcttaca
     4921 gaagcggcta aatatcttgg cgtaccgtat ctatggggcg gtacaacacc gtcaggtttt
     4981 gactgttcgg gatttgtaca gtatgtatgc cgtgcaaacg gtatttcaat acctcgtata
     5041 gctgatgacc aacttcacgg tccgggtaca tatgaaacaa gaggtgaact tcaaccgggt
     5101 gaccttgtat tcttcggaag tggcggttat gcatctcacg ttggtatgta tgtaggcgac
     5161 ggaatgatga ttcatgctcc gtcaacaggt aaggtgattc aatatacttc tattgactca
     5221 agttactatt catcaagatt tttaggcggt aaaagggtaa ttgattaata tgtatagtaa
     5281 aacggactgt aatgttattt gcagttcgtt tttttgtggc taatgatttg aacaaatatg
     5341 attatat
//
LOCUS       DAWACS010000046         5337 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1723_length_5337_cov_2.752934, whole genome shotgun sequence.
ACCESSION   DAWACS010000046 DAWACS010000000
VERSION     DAWACS010000046.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5337)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1723_length_5337_cov_2.752934"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1496 a         1312 c          821 g         1708 t
ORIGIN      
        1 tcattttcag acttgtctgt ttttctttta atactttttc aaatcacaat acactcttat
       61 ccaaccggtg tcaagttctt tactgctctt ttcctgccaa atatagatta aatcattttc
      121 aatcttaacg ctgccgttat ggattttact gtcattatcg tttatcacaa ccgacgatgt
      181 atcaaattgg atataatatg ccggttttgt ttcatcgcca acatcaagtg cgaatttgaa
      241 catatcatta tttttattgt catttactat cgtcaaaccg tcaggataat aatagtaatt
      301 gccgttatat ctgattattg acgcaccgaa cgatttttta ccgtcctcct gtgccgcctc
      361 ggttacaaag tcattccatt cccatgatgc aagttcaaat cttccatttg tgccgctgtc
      421 aactataagc ggtgcaatgt catcatctcc gacaacatca attacaaact gtgcatatgc
      481 gctgttatcc caaagcgaac ttgagtttga cttaaccgta agatgtgtct gtcccgattt
      541 tttcatatta agcttatccg ccgttacggt atacttactt ctcggaagtt cttcccttgc
      601 accgcctgca tatactgccg ttacagtcaa gttctttata accttatcaa cttctctgcc
      661 tatgaacacc attttagtcg gattattttc gtcaagcata tcgtcctcat aatacgtatc
      721 aggtccgctt accgtcaaat atgcgatttt ttcgccgatt tgcggttttt ccgccacttc
      781 aaccgacacg tctttcataa gtgcgaactt aatctcttta tccgatatat ccttatgctt
      841 tttacacata agcgcaagtt tttcttcacc tgttctgata taataatttg tcgacggata
      901 ttttttactt aatccggtct tttcattaag gcttgaaatc aatttttgat aatttccgtt
      961 ttggataagg tcgtgccaat atgtttcgcc tgttttcatt tcgtccgtaa gctcgtccga
     1021 aagcatattc agcattatgc tcatatgcgt actgcacttt gccttattac gatttataac
     1081 gcactgcaca agtgacggca ctattaccac gcacgacaaa acaattacaa ccgctccgac
     1141 accgaattcg accggtttta ttttcttaaa ttttgagccg attgacgaaa atatatttcc
     1201 caattttttc ttaatctttt gtaaatttat ttttttcaaa ttattttttt tcaaaactgc
     1261 acctcaaatt ataattttct gtaaaccgca aaatacattg ttgtaaagca cgccaaaccg
     1321 cctatcaaat ttgaaatcgg ctctgccaaa aatactccgt ttactccgaa ccatatcgga
     1381 agaagaagta taagcggtat taccactatt ccctttctca atatagaaaa gaatatcgca
     1441 aacttcgctt ttcctattcc gacaaacacc gtctgtcccg taaacataaa cgccataagg
     1501 aaaaatccga aaaaatatat atgcaatgac ggtacaccgg cagttataag tgccgcatcg
     1561 gcagtgaata ttcctataag gaatttcgga atcagcaaaa ttataaacca tgccactgta
     1621 ctgtatataa taagcattgc cgccaaatac cgtatcgcct ctttaacccg tccgttttca
     1681 cctgcaccgt agttaaagcc gattatcggc tgtgcaccgt ttcccaaacc cgttacaggc
     1741 atttgcaaaa cttctcttat agaattgatt atagtcataa ctccgacata cacgtccgaa
     1801 ccgtaattct gcaaacttac gttacacgca atctgtaccg cactgtttgt caccgacatt
     1861 gtaaatcccg aaagccctaa aacaagtatc ttctttacac gttttgcctg acagcgcatt
     1921 gcggaaagtt ttatttttat aatcgtattt ttgccggtta aaaatttaaa cgtccaaacc
     1981 gacgcggcaa gctgtgacaa gactgtcgca agtgccgcac cctgtacgcc cattatatat
     2041 ataaatatcg ggtctaaaat aatattcagc accgctccga ttacgacggt aagcataccc
     2101 gttttaccga agccctgtgc attgatgaac gaattaagtc ccaaactcat cataacaaac
     2161 acgttaccca aaaggtaaat cgttatatat gaatttgcat attcaatcgt attttcactt
     2221 gcacccaaaa gatacaaaag cggtcttttt acgattaatc cgacaaccgt aagacacaaa
     2281 ccgaatatta caagcagtgt cacgctgttg ccgagtatcg cctcagcctc tttttcgttg
     2341 ccctcaccgc gttttatcga aaacaacggc gcaccaccca taccgataag gtttgcgaaa
     2401 gcgattacaa cagttataat cggcagacaa actccgacgc ctgtcaatgc aagtgacgaa
     2461 ttttcgccca ttcttcctat ataaattcgg tctactatac tgtaaagtac attgataagc
     2521 tgcgcaagtg tcatcggaat agaaagtctt aatatattag atattatact tccctttgaa
     2581 aaatcgttta ctacttgttt agccattctt ccgccgcctc aatttgtgtt tgtatttctt
     2641 tttcaagtat tccgcccgaa ttttcttcga ccttacaaat cactttactt tgagtcgtaa
     2701 acatcactcg gacaaaaaac ttatcctcga ctttatcgta tattcttccg gcactgagcg
     2761 taccgccggc aatactcgcc gggctgtttg cgatatatcc gaccttgccg ataatcggca
     2821 attttacggt tatcgcctct gaatcatatt tgtttgacgg ctcttttacg cagataagca
     2881 cgtttcctat tgcaaaaggc tttttatgat aataattcgc aaagcccgtt accgttacat
     2941 acaaatcctc cataaatccg cccccattca tcaatatatt atccgaacct gtaaacaggt
     3001 tcaaaaatca tatttgttat tatatcattt tatatttgtt ttgtctactt tttttactac
     3061 tccttcaccg tcaaataaag gaataaagct ttccgaaaca gtaccgccct cctgcacaaa
     3121 agcattttcg acaccgattt caaccgcaaa gtcaagcact tcgtcatatt cgctttccgt
     3181 aacttttctg tttatttcgg gatattttaa aacactttca agcggtgtgt actgattcat
     3241 tatgctcata taaatatcat cgccgtacgt gttatacaga tactcaatca ctctttttga
     3301 attgtcggta tatgacggca aaacaagatg tcttacgatt attccgcgtt tcatcatacc
     3361 gttttcgtca aattcacaac ttccgacttg tcttaccatt tcatctatcg ctctttttgc
     3421 gtgtatcgga taatcctctg cttttgaata cttttttgca agtacgctgt cggcatattt
     3481 aaaatccggt aaataaatat ctatataacc gtcaagcatt ttcagtgtat caacgctttc
     3541 atagccgccg cagttataca caatcggaat atcaagccct tttaccgtat cgagtgcctt
     3601 tataatatgc ggtacataat gagtcggtgt gacaagattt atattgtttg cgcctttatc
     3661 ctgaagttca agaaatattt ccgtcagcct gtccgacgtt atttcaaagc ctttatgctc
     3721 cgaagatatt tctctgttct gacaatacac gcacccaaga ttacatcccg aaaagaacac
     3781 tgttcccgaa ccgctctcac cggaaataca cggttcttcc cacatatgca gtgcggcacg
     3841 tgccacataa agcttatcac tctgaccgca tacaccgact gtcctgcttc tgtcaacacc
     3901 gcagtttctc ggacaaagtc tacattctct gtaataattc atcgcaatat ttccataacc
     3961 ttgtcaaatg ccgccgctgt cttttccaaa tcagccttag tatgtgcgtc agacaagaac
     4021 agcgactcaa attgcgacgg tgcaagataa ataccctgtt taagcatttc gttaaagtat
     4081 actttgaatt tttcggtgtc cgactttgac gcagtttcaa aatttgttac gtcattgtcc
     4141 gtaaagaata cactcatcat tgagcctact ctgtttacac gagcattcac accatatttt
     4201 tcagccgact ttttaaattc actctcaaga tatgcaccct tttgttcaat ttcttcatat
     4261 atataggggt tttcctgcaa atatttaatc tgtgcgttac ccgccgccat tgcaagcgga
     4321 ttacccgaca atgtacctgc ctgataaaca ggacctgtcg gcgaaacaaa ttccataatc
     4381 tcacgtttac cgccgtatgc gccgacaggc ataccgccgc cgataatttt tccgaatgtg
     4441 acaatatccg cgtccaaacc aaatctgcct attgcacccg acgcagataa tctaaatccc
     4501 gtcataacct cgtccacaat aaacacgctt ccgtactttt tcgttatctc gcgaattttc
     4561 tgcaaatatc cgtctttagg cggtacaacg cccatatttc ctgctacagg ctcaattata
     4621 actccggcaa tttcattacc cattttttca aatgcgtcct ctattgcgtc aatatcattg
     4681 tatggtaaaa caagcgtata ctttgcaaaa tcttcaggca cacctgccga tgacgcactt
     4741 ccgaaagttg ccgcacccga accggctttt ataaggagcg agtcgctgtg tccgtgataa
     4801 cagccctcaa actttattac tatatttctg cccgtgtaac ctcttgccag acgtatcgca
     4861 gacatagttg cctccgtacc cgaattaacc attctcacaa catcaacaca aggaacaagc
     4921 gaacaaattc tctttgcaag ctccgtttcg gcaaggcaag gtgcaccgaa agacatacct
     4981 ttatcgcaag cctccttaac cgcctccaaa acgatttcct tacagtgtcc caaaaacata
     5041 ggcccccatg agccgacata atctatatat tcgtttccgt caatatcgta tatcttactt
     5101 ccgtttgcac gttctataaa aagcggataa gttccgacat tcttaaacgc tcttacaggc
     5161 gaattaactc cgccgggtat atattttttt gcctcgtcaa aggctgtttt gctttttgat
     5221 gtatccacaa tatatcattc cttttctaaa cagataaaaa aattatacac cctacttaat
     5281 ttattgtcaa ttcccttgat ttttcgatat aatagtgtta aaatagacaa gatatat
//
LOCUS       DAWACS010000047         5294 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1745_length_5294_cov_3.200993, whole genome shotgun sequence.
ACCESSION   DAWACS010000047 DAWACS010000000
VERSION     DAWACS010000047.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5294)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1745_length_5294_cov_3.200993"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2024..2033
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1240 a         1335 c          901 g         1808 t
ORIGIN      
        1 acgatactgc cctttaaacg tcctgttgca acaggacagt ttgccactgc gtgcgctctt
       61 acgacttcac ccgccattgc caaacctcgc tgtattttat cgcccgacgc atactgtgtc
      121 agcttgtcaa caacgtcgtc tatcccctcg attgaaaaat tcatttcagc ctactcctct
      181 caagcattgc taccaaaccg ctgtcccatt tctgcacata tgttatatcg tatatatcgc
      241 cgtcatattc aaccttactg ccgacattta catcgtccga catatcacag aacatacgca
      301 tttgacattc tatatcaaga ccgtattctt ctcttgctct gccaccactg tacggttgta
      361 tatcggcttt gatttcggat aataccgtct tttcagcttt gcctgtatag tcgtcaatct
      421 cgtattctgc aattatgacg tctttattat aaaacctact gaataccgac atcactcgga
      481 acacgcccct ttcgtttgcg gaacgggtca agacgtttat aataattgct gaaaatctca
      541 tcattatcat tttcggcata tgttacggaa cgttcgccct cgcttacgct cttgactact
      601 tcgggacttt tactgtcccc gtagcctttt attctataca tatccgccgc aatcttcgga
      661 acaagacttt caagctgacg tggcagtaca tcaatatgac aatacgccat aatcatatta
      721 accgtgtcct caatcaaaaa ggacaacaag ctgtcttgct cgtcgtcctt aattcccaac
      781 aacattttta gtgtccccaa ctgttccata ttattcaccg cttacaacgt cggcactgcc
      841 cgactttctc gctttgccgt ctgcggtaac ttccgcaact gtaatcttgt gaccgtttgt
      901 cgcagtgatt tcgtcaccgt tgttaaactc tgtccacttc gacaaatcgt cgtcatacgc
      961 aacacttgga gcggtgcttg cggcagcctt gtaaaccaac ttgtgaccgc cgataggctt
     1021 tggcgatacc gtaataacgg tgttgcctgt tgtgcctgca accgattcaa ctgtcaattc
     1081 gccgagtgtc ggaacaccgt tcttaaatgc ggcaaatgcg tcgtccttga caacaaggaa
     1141 acctaaacgc atagtagcct tgattgcaac catatcttgc tcggcaagtg atagcggttt
     1201 accgtcactg tcaagagtgc cttgtagtgt tgcctctgta agaatttcat agttgatacc
     1261 tgcacgcata ccgacaacgg catacttgaa gttacctgtg ataatatcgg cacgtttatt
     1321 gtcccacgca ccgttacgta caaattcgat aggctgaccg tacagctcac cgcctgttgt
     1381 accgttgaca tatgcaggtg cgccgtttgc gtcacgcaat tttctcagca tattcttaac
     1441 accgatacga ccgataaatc ccgacgggtc atagccgttt tcttcaatca tcgacattgc
     1501 gtcagacata gcaatatcaa tatttgcgtt gtctgtaaca accatatgct tactgtcgat
     1561 agcgttcata atgtttgtct tgaacggtga atttgtaccg aaaatgcacg ccgcgtcaat
     1621 cgctctgtag aatgcctctg cgatttctgg ctttagttct tcaaatacgc tgatagtcgt
     1681 atcttccaac ttttcttttg ttaccggaat aataacggct aacttcttag cctcgatttc
     1741 agggtgaatc caagtagcac cgcttgtctt aattctttca ccctcaccga cccagtaagc
     1801 acccggaccg tctgtaagta cgttaaactt ctttttctcg tgtttcattt cctcgacttt
     1861 cgccattctt aaaacacttg aaccccttgt caccattttg atgatgtctg ttgcttgctc
     1921 gacaggcaca aagcctgtca attcattttt caaataaccc atttatttca ctccttatct
     1981 ctgattttct ctgattatgt ccataaaact gcctgtgttg tgannnnnnn nnncggtgtt
     2041 tttcccttta aacgctcggt aacacctgct tgtacatctt tgtcatagct ttcttttatc
     2101 ttgtcaataa ccgcctttgt gctatccttg tcctctgcta tgatgtactt tgcaatctcg
     2161 gcggacagtc cgactttggc aagttccgtt tcggcatatg caacgatttt ttcacgttca
     2221 aactctgcct ttgctttttc aaattcttct cgttccttgt cgtcgtcctc tttttttctt
     2281 tgctcgtttg tcaacttggc ttttctcatg ccctcgtttt cagcgtcctt tagcttttgc
     2341 tcaagttcct tttcccactc cgctttcgcc ttagctatcg cttcgtcaat cgccttttga
     2401 ttgtcgccgt ctttttgttc ggttgacttc tgctctgtgg acttctgctc tgtggacttc
     2461 tcttgctctt gattttctgt ttgctctgct gtatctgcca ttcaaatcat tcctttctga
     2521 aaaattgtat aaaaataaga cgtataaccc cacgtctaac agggagataa tcggatcacc
     2581 attcctttct tctatgtgta tgttgtgcct actctcacac tatcaccgcc tttcagtgta
     2641 tcaaaaaagc acgtccgaaa acgtgctttt gcgtatattt agttattttt atctgaaaat
     2701 tatctcattg cctttatcca caaaaccatc aggaatattt tgtagtacaa tccacggtga
     2761 gccacctttt gaaaagcacc ctcttgtttt ttcaacttta aactcttttg tcgtatatac
     2821 ttgcttatgc ttattgatga tttgcaatag tttcgcattc gtaaaatctt ccttgcacgg
     2881 agtgcaatcc acaataaatt gattgtttat tttttgaaca tttattattt cacacataac
     2941 tatcactcca aatacaataa atttcgtacg tcttttaatt ttttacccgt tttatgtaat
     3001 ctatataccc cgtcccttgc gagtaccatt ctttcgctgt ttgataaatt gggtgttaat
     3061 attgccgccc tcaaactcgc ctgcacttct tctaaaatat catcactttg cgtctttccg
     3121 ttcaaaaatg ccgcacggtg tccgattatt tcgtgagcta tggtaccttt catagaaaca
     3181 ttactgtttg gattggattg ctttttctct gacggatata catcagttcc aattcgtaaa
     3241 acgtccgcta aagagccata tgctgtacaa tcataatcaa cataataaat acgttctctt
     3301 ggcataccca aagaaaccgc ataatcttta atgctgtcta tttgttcctg tgaaagattt
     3361 ttctcgtttc ttcttccgcc cgtagatact ttcaatgtat ctttatctac ctttattata
     3421 ccacgttttt cactatttgc aacatatttt aacgcatttt tctgttcgtc cgacaaactg
     3481 tttttccatt cgtcaaacgt catacttccg tcaaccttgt aattttcgcc agtgagcgga
     3541 tcgcgtgcaa tacgacttgt caaattcacg tctgccataa tcgtaacaca ccgacaacgt
     3601 gggtgtatcg gtgggaagtt ttcgccctca acggctttgt cggtatcaaa cacgctaccg
     3661 tcaagacttc cgcacctgtc acacgtcaat tcagacagtg ccgcaacaaa acgatactgt
     3721 tttataccga tttcctcata cgccatcttt tgaccttggt tcataaaatg cgccgtttcg
     3781 ctcctcacaa gcgtttcggc tgatgttcgt attccacccg gtgcagtatc tttgacgtaa
     3841 tcaatcagct tgtcggtcat acggcttaca ctgtgaccgc tgattatacc gtcctcaatc
     3901 gtctgtccga ctgcctgtat aaatctgtcg ttatgtatcc acactctctc gctgtagttg
     3961 tgaccgtgcc acggctcact taacacttta ttaaccgcct tttgcggtac aagtggaaaa
     4021 tcaataccgc agtttaaacc ttgtgcggta tcaaaaatat tcgtataata cgccgtcttt
     4081 accgcactgt catacagttt cttttgctcc tttatagcct cgtttgcaac gtgcctaaag
     4141 taaatatata cattacgttt cagtccctct aatcggctaa ttctcgcacc atatgcctgt
     4201 gcatttatgc ggtttagaat ttcctttttg actgtcttgt cgtctgtttc gtcgtacagt
     4261 tcaagcagtt cttcgtactg tttgtcgctg tcggctatac tcatcagccg acgtgcctct
     4321 ttttcgggta tatcggttga aatataggct ttaaacgttt tctcaatatc attgtttaca
     4381 ttcttgattg ctcgctcata tgccttaatt acaccgtcct taacgctgtc cgcttgcgat
     4441 tgtaaatatg tttcaacttc aacggcacgt tttacccaat atgccttact cttcattgta
     4501 gtttactttc cttgccgaaa tttctgcgat acgcatatct tcggcggact tttccgcttg
     4561 ctctctgcgt gcaatttcaa cttcttcctt tgcgtctgtg atgaatggca aacgctctaa
     4621 taatgtttcg tcagacgcaa gacctttgag gtaattaatc atctgtgcta tttcaagttc
     4681 gtttgcaggc aagttatatg taaatcctat atcaactctg tgcgacggca cttctttcat
     4741 tgcgtttaat gtcactaaga aattgttgta aatctctaaa cgttttctta atgtcttagc
     4801 aaaattacgt tctttgttct tgacgtgctg ttcaaatccc aacagcttgt actttattgc
     4861 cacgcccgac aaattgttgc cgaaactttc gtccgacaaa tcgggaacgt gtgacagacg
     4921 gtgtatatcg tccttgatgt catcacgcaa cacctttgta tcagcctcgt tcagcacctt
     4981 tgacagatac tccgctttcg cgtcgccgtc acccattaag atacgttcta ccaataattt
     5041 ttttgcctgt tcggtgtcaa ggtcgcagtt gcacaaaaac aacagtgaat tgacgaattg
     5101 ttccttgtca ttaattctat ctgacatcaa cacattgtat gcgtcaatct gcgttataag
     5161 ctgttcaaaa tcgccctgca tttccgtatt atttctgtat tcgataatag gtacatcaaa
     5221 aaagtaatgc ggttcaacat tttgcaatga caacgccgaa taactgtcaa gacctgtgta
     5281 tgtatatata aacg
//
LOCUS       DAWACS010000048         5244 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1764_length_5244_cov_2.165157, whole genome shotgun sequence.
ACCESSION   DAWACS010000048 DAWACS010000000
VERSION     DAWACS010000048.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5244)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1764_length_5244_cov_2.165157"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1433 a         1274 c          854 g         1683 t
ORIGIN      
        1 ttaattgcgt ttttatttat cgaaccgaac agttcattta taacgtccgt ctgaattttt
       61 tcgcgaacat cgtcagccgt tttgaaatgt gcgtaaaatg tacttctgtt aagatcagcc
      121 gtattgacaa tatcggtaac ggttatcttc ttattctttt gcataagttc gacatacgca
      181 tggacaatcg ctttttcaga acgtgtaaaa ttcctatacg ttttcttttt ctttacttct
      241 tccatgtttt tttatcctcc taagtacaac gaacgcagca ataagaaaaa cagccgttcc
      301 cccacaaatc gcaatcggta caatcggcga tattttctta taaagcaata catatttaca
      361 aggttttgcc gtttcaaaaa ccgcataact gccgtcaatc gtacaatcaa ccatactcca
      421 tttaccgtca atctgtactg caatctttgc agcctttttc ttatcatcac gaatatgtac
      481 ttttataaca tcataagcat ctttacttaa tgtaaatgag tatgccttac cgtttttata
      541 tccgtcaaaa acgtggtcaa catcttcctc tttgagcgac aatgttgtat tatccgcaaa
      601 tacgctttcg gcaagcatta acggcggttc ttcgcctgat gaaatagtcg ttgttgcctt
      661 gttgtattct gccgaaaatg tagtgtgacg tatgatagga cttttcacgt ccttatccca
      721 catcggaact tcactgccgt cagcggtaag cttcggtata tcgctgtcat ttaatacagt
      781 accgtaatca actgtcattg acttcaattc tttatcttta tccttgaata taacattgaa
      841 atgaaatctt gaaagttctg ttgactttga ctgcaaaatc gcacctatac tctcggcgtc
      901 attttgagcc aaactcttta tttgcggaag gaatttatct ccgtccgcca tatagaaatt
      961 accgttatcc aaaagcggca tacggctcgg gaaactgccg tcagacgttg tcatatcgtc
     1021 aggcttgaca gcctctgctc tgccctcaag gttcattccg cttatacctg aaagtccctc
     1081 atcaataaaa taacaacccg taacatcacc gtcaacaaat cccactattg cgccggcatc
     1141 atcttttttg ccgtccaatc tcggaagcga atatgacgta ttaatatttt tagccgagcc
     1201 ggcaataccg ccgatatggt cgttaccgtc aacatcattt atcgcaatac aattatctat
     1261 atttccgctt gtatatcctg ccgtaccacc gctgtaactg ccgtcattta cagtaatttc
     1321 gcctgttgta agcgaatttt ttatatcgcc tatatcactc ttaccgcaaa taccgcccgc
     1381 atatttcgat tctgcggtta cattaccgaa acttatacaa gaatcaatta cagctttcaa
     1441 aacagaatcg gaatttactt tagtgccgtc aggcaaagta agattttcgc cggaacgaac
     1501 tatcgactca aagccgacac atccgactac accgcctgtc gattcggttc cgtctacggt
     1561 accgttattt atacaatatt tcacaagtgc cgtatcggca ttttcatcac cgataagtct
     1621 tggcaacgaa atatccgtac tcttcaaatc acttattgca gttttttcat catctgcttc
     1681 cgccgcttta gccgttgtga acaacatatc ctttagcata ccgacaacag acggttcaac
     1741 gtcataactg ctgtcaagct caccgtcagg tgttacgttg tagcccggat caatatcgtc
     1801 ctcagatttt acactttccg aagaagtatc attattgctg tcgcttccac cgctgttacc
     1861 tccgctactg tcactaccgc cattgcttgg cgttgccgta ggtcttatca tagggcctaa
     1921 tgtaggcaac ggctttaacg taggaagtgt tgaacccaac ttatcaagta cgtctttaaa
     1981 tgatttaatt gtatctttta atcctttcaa ttccttgctc atattcaaaa caaggttatc
     2041 aagattctcc attgtatcac tgaccgaatc accgacatca cccgatacat atttaagctg
     2101 tcttaaaagt ttcttcaatt cttcatttgt atcgtcaaga ttatcaatta catcatcaat
     2161 atctttttcc atcttgtcaa gacgatttga aagtttgtct tttgaatcgt caacatcatc
     2221 tttgccctta tcaagcgaat cgttcaaatt gtcgataagg tctgacaatt catcaccctt
     2281 gccgtcaaat tcgtcaagga aatcatcaag cgattcaaga gaagtattaa gcgaatcact
     2341 cacatctctg attgactgct ttgattcgtc tgtaaacgaa gatatatctc ctcttgcttg
     2401 gtcaagcgta tctttcagcg aacctgtcac attactgtca gacgccgaat ttaccgcatt
     2461 tgttatactg tccatcatat tgacggctga atccgtaaga acgtcaagcc ttgaacgtgt
     2521 attatttttt gcgtcaccca agcctaaaag cgacataagt cccgatacat catttgaacc
     2581 tgtcaacagt ttcaaaccgt attcaattaa ctttgttctt gcacttgaaa tatcattttg
     2641 aaatgtatct atatcttttt ctatcgccgc ttttgcactc tgatacgaca aatcaatatc
     2701 tgtatacggt tcaaatcgac cgactacacc gccgacactt cttcttccct gaacagcggc
     2761 attattgata cattctctta cttcaccgct ttgtgtaccg actataccgc cgatattgtc
     2821 gccgaagctt tcgccgccga cagcgccgtt atttgtacaa ataacaaccg caccctcgtt
     2881 attaccgcag ataccgccgg cgtttaccgt attttcgttt gcatccgggc atacctttcc
     2941 gtcatttcga cttagcttta ttctgccctc attgtaaccg actataccgc ctgtttcact
     3001 gtcaccaaca actgtagccg aatttgcgga agaatcaaca acaccggtca ttgcattgta
     3061 gccgacaagt cctccgacct gtttctgacc tttgatattt ccgccgtatg aacagtttac
     3121 gattttaccc tcgttatagc cgacaagtcc gcctgcacct tttgagcctt tatcaaaatt
     3181 ttgagtattt atatcagtct tactcaaaat cgaggtggct ttttgtctaa aaaccgattc
     3241 cgtatcggtt ccgactgtaa cctttatatc tcctgttacg ttcaaatctc tgatctgacc
     3301 ttcttttgtc actgaacaga aaagtccttt atttgaaccc tcaaatgaaa gcttgacatt
     3361 ctttatactg tgaccgcctc cctcgaaagt tccgcagaat acctccgcac tttttatttc
     3421 cacaccgttt aaatcaatat cattttgtaa tacgaacttc ttattttttg aatactcgtc
     3481 atatacgcac ttttcaaaaa attctttaag gtcgttcact gtcgatattg caacagtgtc
     3541 gtctgcctgt acaatgcaca taggtaatat attcagcatt ataacaagtg cagtcagaaa
     3601 agatatgatt ttattgttct tcttcgttgt tctcattatc ctcatcctcc tgcttttcat
     3661 ctatttttat attatcttcc ttactctctg taagcaacaa atcctgttga tttccaagtt
     3721 cgactttcgc gtctatatca ccctttacca agccgtagaa tacaccgtcc ataaagcctg
     3781 aaacgtgacc tctgacacga ccgttgacga acataattcc ggacatttct ctcttcgccg
     3841 ctttgtcgtc atctgttgcc tttttaccga atgcggtctt ttcaataaac ttatcgcaca
     3901 acagtacaat ttgcggaagt accatcatta ctataactat tgatattaac gttccgcgtc
     3961 caagtgccgt accgagtgcc gatataatag catttgtcga aatcatacct attaaaaatc
     4021 ccgcaagtgt aaggattgaa cctgatgtaa gaattgtcgg gaacgattgg ttaagtgcgg
     4081 ttgacgccgc ctgtttatta tccattgttt cctttaattc aaggtatctg ttcgtaaata
     4141 cgatagcata gtcaatcgta gcacccatct gaattgaact tacaaccaag tatgcaagga
     4201 agtacaatct ctgccctgta aggaacggaa ttgagaagtt aatccacaca cttccctgta
     4261 tcgcaagtac caaaagcacc ggaacacctg ccgaacggaa tgtgaacatc aatatcaaaa
     4321 gcaatgccaa gattgttata agattaatct tattattgtc tccgccgaac gaatcaccca
     4381 agtcacggct gtttacgctg tcgctgacaa gcaaacattc gtcataatac ttttcaacgt
     4441 cacttctgac gtctttaagc atttgatatg cctcgtcact ttcaatgtca catttatatg
     4501 taaagattat tcttgaataa ttatcgcttt caagctggtc tttcgcgtct gtcagcttgt
     4561 cgtaaaggtt gattacaccc aaatccatct tctcgtgtac atacataaac aagtcgatta
     4621 taggcacttc gtaatcattt acatcaccga atattgcatt gtattcatcg tgttccgcac
     4681 cgtatgcctg gaacaaaagg caacacaaat cataatcaat attcataagc tttgaagttt
     4741 ctcttgcgtt gattttttcg gtaagtatgt ggtcgtcgtc aagctccgta tttgcaagac
     4801 ccaaagccga atttatgctc ggattttcct cgacaagcga tataactttc gcctctctgt
     4861 catagtcacc ggacggaaca attacagcaa gctgatggtc cgtaccgaaa atttcgtcaa
     4921 cctttcgttt tgcgattgag ttttcggtcg gctttttaag ctgttcggct gtggcgtcaa
     4981 aagcatagtt actcattgac gaaagcaccg caccgactgc tataatcgca ataaatatgt
     5041 aagggacaat gttctttaat ttaataacaa ccttacacca tttctcgata ctcggcacaa
     5101 aagaacggtg agttgtttta tcaatcttat ccgaaaatgc caaaagcaag ccgggcataa
     5161 taaagaacac cgtaacaaga ctgcatatga tacctttaca aagtaccaaa cccatatcca
     5221 taccgattct aagctgcatt acca
//
LOCUS       DAWACS010000049         5212 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1773_length_5212_cov_2.304828, whole genome shotgun sequence.
ACCESSION   DAWACS010000049 DAWACS010000000
VERSION     DAWACS010000049.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1773_length_5212_cov_2.304828"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    663..672
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1716 a          882 c         1171 g         1433 t
ORIGIN      
        1 atttcgaaga aaagaaattt aaaaacgttt ctgaagatat atacgatgac tttgtaaaag
       61 ccgaaaaact tttggcattt aacgcaccgc ttatgcagtt cgcggcatac ggctgtatgc
      121 ttttcatatc gtggttcggt gcaaagctta tcgttgcgtc aactatgaca acaggtgagc
      181 ttacaagcct tattgcatat acaatgcaaa ttgttatgaa ccttatgatg ctttcaatgg
      241 tgttcgtaat gattacaatg gcaagagcgt catctgaaag aatcgtcgaa atacttgacg
      301 aaaagagcga tcttacaaat cctgaaaatc ctgtatatga aattaaaaac ggcgatattt
      361 cattcagaga tgtaaacttc ggatattcaa agaaaaagag aaaactctgc cttaagaata
      421 taaatcttga tattaaatcg ggtgaaactg tcggaattat aggcggtacg ggaagttcaa
      481 agtcaagcct tgtacagctt atcccaagac tttacgatac aaccgacggt gaagtgattg
      541 tcggtgacag aaacgtaaag gaatatgata ttgagtcatt gcgtgacgag gttgcaatgg
      601 tacttcagaa aaatgtgttg ttctccggaa caatcaaaga aaacttgcgt tggggtaatg
      661 acnnnnnnnn nnatgtaagc ttgcacaagc cgatgaattt attcagacat tccctgataa
      721 gtatgatact tatatcgaac agggcggttc aaatgtatcg ggcggtcaga aacaaagact
      781 ttgtatcgca agagctttgt taaagaaacc gaaaatcctt attttggacg acagtacaag
      841 tgcggttgat acaaagacag atgcaagtat cagacgtgcg ttccgtgaag aaataccgaa
      901 tacaacaaag attattattg cacagagaat ttcatctgtc gaagacgcag ataagataat
      961 tgttatggat aacggtgaaa taaacggaat aggtactcac gaagaacttt tgaagaacaa
     1021 cgaaatatat agtgaagtat ataattcaca ggtgaaagga ggcggtgacg atgaataaac
     1081 acggtgcaca aaataccggt aagacaatca aacgtattct ttcatatatg aagaaataca
     1141 aattcagatt tatctttgta ttgatatgta tattgctgag tgccgttgca aacgttgccg
     1201 gctcaatgtt tatcggtacg cttatcgatg actatataac accgcttttg ggcagtacaa
     1261 accctgtatt cacgggcttg cttaaagcaa tctgcgtaat gggtattgtg tatgcagtgg
     1321 gaatacttgc tacattgttt tataacagaa ctatggtatc aatcgcacag ggtgtgttaa
     1381 agaaaattcg tgacgatatg tttactcata tgcagaaatt gccgattaag tatttcgata
     1441 cacatactca cggcgatata atgagttact acacaaacga tacagatact ctaagacaga
     1501 tgatggcaca gagtattccg cagatgtttt catcggcagt cacaattata tcggtagttg
     1561 ttgcgatgtt tatatcgaat gtttatcttg caatatttgt gttggtgttt gcgacctgta
     1621 tgatgtttgt aacaaagaag attgcaggag cgagcggaag attttttgta aaacaacagc
     1681 agtccatcgg taaggtaaac ggttatatcg aagaaatgat taacggtcag aaggtcgtta
     1741 aggtgttctg tcacgaagaa cattcaaaag aagattttga tgagattaac gaagagcttt
     1801 gtgaaaacgc aacagaggct aataaatacg caaatatcct aatgccgatt atggctaatt
     1861 tgggtaacct gcagtatgtg cttatagcta ttatcggcgg tgcacttgca ctaaacggtg
     1921 ttgcgggact tacacttggc gttatagcgt catttttgca actttcaaaa tcatttacaa
     1981 tgccgataaa ccaaatatcg caacagctta actcaatcgt tatggcactt gccggagcgg
     2041 aaagaatatt caatcttatg gatgaagaag tagaacagga caacggcgat gttacacttg
     2101 tcaatgttaa gtacgacaga aacgacaata tagttgaaac ggaagaaaag acatatatgt
     2161 gggcttggaa agaagttcaa aaggacggca gtgttaaata tacacgtctt acgggtgaag
     2221 ttaaattctt tgatgttgat ttcggatata atcccgaaaa aatcgtactt cacgatataa
     2281 cattgtatgc cgaccacggc gaaaaggttg cgtttgttgg tgcgacaggt gcgggtaaaa
     2341 ctacaattac caaccttata aatcgtttct atgatattgc cgacggtacg ataacttatg
     2401 acggaattaa tattaaccgt atcaaaaaga aagatttaag acgttcgctc ggtatcgttt
     2461 tacaggatac aaacttgttt acgggtacaa ttaaggaaaa tatccgatac ggtaatcttg
     2521 acgcaacgga cgaagaagtt tatgcggcgg caagacttgc gaatgcggat gagtttataa
     2581 atgtgttgcc tgaaggatac gatacagtta tttcgggtga cggcggcagc ttgtcgcaag
     2641 gtcaaagaca gcttttgtca atcgcaagag cggcggttgc agacccgccc gtaatgatac
     2701 ttgacgaggc aacttcaagt attgatacaa gaacagaggc gatagttcaa agaggtatgg
     2761 acgcacttat gacgggacgt acagtatttg ttatagcaca cagactttca acggtaagaa
     2821 attcagatgt aattatggtg cttgaactcg gaagaattat cgaacgcggt aatcacgaac
     2881 agcttattgc acaaaaaggt aagtattatc aactttacac aggtgcattt gaacttgaat
     2941 aaaaagaact gtcgataaat tgtccgtaac gtgcaaaatg ttatttttat gtttatgagt
     3001 tgattaaaaa ataaaacaaa taatagtcaa gcctatcttt gatatacggc ttgactatgc
     3061 actttttgtg acaataaact ctaacgtacc aatgtacgcc gacgagttca ttttcgcaaa
     3121 aaacgaactg atttctcaac agtccttgat tgaaataccc atttgcttaa tgcaaatggg
     3181 tattttaata tgaaagtata atatatttcg ttgctttttt ctacaatgta cttgacgata
     3241 gaggcgaatt ttgttaaaat tataataatg gttaaataac gaggaggatt acatgatgaa
     3301 aaagttaatg cttggaaatg aagcggttgc acgcggtgct tatgaggccg gtgtaagcgt
     3361 tgtttcctca tatcccggca caccgagtac cgagattaca gaaaatatag ttaaatatga
     3421 cgatatatac gttgaatggg cgccgaatga aaaggttgcg gcagaagttg caatcggtgc
     3481 gtcaatcgcc ggcgcaagaa gtatgtcttg tatgaaacac gtcggtctta acgtaatggc
     3541 tgaccctgtt ttcactgcaa gctatacagg agtaaacggt ggtcttgtat tgtgcgttgc
     3601 cgatgaccaa ggtatgcact catcacagaa tgaacaggat tcacgtcact atgcaaaggc
     3661 atctaaaata cttatgttag agccgtctga cagcggagaa tgtaaggagt tcacaaagac
     3721 tgcatatgaa ctttccgaaa aatacgatac acctgtattt ttaagacttt caacaagagt
     3781 ttctcattca cagagtcttg ttgaggaaga agaaaagcag gaagttgcat taaaggatta
     3841 cgagaaaaat ccgcagaagt atgtaatgat gccgggtaat gcgataaagc gtcacgttgt
     3901 cgttgaggac agaataaacg cactaaagga aatggctgaa acatcacctc ttaacagagt
     3961 tgaagaaaac ggctcaaaaa tcggtattat cgcaggcggt atcgcatata tgtatgcaaa
     4021 agaagcactt ggcgataagg ctgattattt gaaaatcggt atagtttatc cgctacctga
     4081 aaagaagata aaagaatttg cgtcaaagta tgataaagtt ttcgttatcg aagaacttga
     4141 ccctgttatc gaagatttct gtaagagcat cggagttgat gtaataggca aggacgtatt
     4201 tacacttcaa ggcgaatata caccgtcaat gattaagaaa gcagttttgg atatagaccc
     4261 acctgaattt gacgctatag acgagcctac acctgttcgt cctcctgtaa tgtgtgcagg
     4321 ttgtccgcac agaggtacat tttatgtact aaagaaactc ggtcttgttg tttcgggtga
     4381 tataggctgt tatacacttg gtgcaacacc tcctctgcaa agcgttgaca caacaatttg
     4441 tatgggtgct tcaatcagtg ccgcacacgg tatggcaaaa gcaagaggtg ctgaatttaa
     4501 taagaaactt gtatcagtaa tcggcgattc aacatttatg cactctggta ttacaggtct
     4561 tgttgacatt gtatacaaca agggtaacaa tactgttata attcttgaca actcaatcac
     4621 aggtatgaca ggtcaccagg ataaccctac aacaggttac acaatccgta aggaagaaac
     4681 aaagcaagtt aatttgataa cactttgcaa gtcaatcggt attgaacacg ttgtcgttgc
     4741 agacccgttt gacgtaaaga attttgaaaa ggtcgtaaaa gaagaagttg aaagagaaga
     4801 accttcggtt attatcgcac aaagaccttg cgcacttctt ccgaatatgc gtaaaaagta
     4861 ttcgggtcac tgtcacatca cagacaagtg taagaagtgt aagatgtgta tgaaactcgg
     4921 ctgtcctgca atttcattag acggcgatac tgttaagatt gacacaacac agtgtaacgg
     4981 ctgcggacta tgtacaaatg tctgtccgtt tggtgcgatt gaaaaggagg aaaagtagaa
     5041 tgaacataat gatagtcggt gtcggcggac aaggtacact tcttgcaagc cgtatacttg
     5101 gtaaggttgc aattaaagag ggttatgacg ttaaagtcag cgaggttcac ggtatgagcc
     5161 aacgcggcgg cagtgttgtt acatatgtaa agtacggtga taaggtttat tc
//
LOCUS       DAWACS010000050         5165 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1792_length_5165_cov_2.304892, whole genome shotgun sequence.
ACCESSION   DAWACS010000050 DAWACS010000000
VERSION     DAWACS010000050.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5165)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1792_length_5165_cov_2.304892"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4010..4019
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1712 a          833 c         1282 g         1328 t
ORIGIN      
        1 cgccagagga cagagaggtt atgctgttat tggtattgga taatcatatg acggacgaag
       61 aactatataa agcgatgcaa gagtaaaatt gcccacagca caatttttcg cttggggcag
      121 tatacacata cggcaccgca agctttaaaa tcgcactgtg aacaatttgt agaaagaggg
      181 gaaaataatg atatttggtg tagactatta tcccgaacat tggaacagag atgagtggga
      241 aagtcaagct aaacttatgc aagatggcgg atttaacact gtaagaatgg gcgaatttgc
      301 ttggaaactt tttgaggaaa aagagggtga atttgatttt tcattccttg atgacgcaat
      361 agagatactt gcaaaatacg gtataaaaac catactcgga acaccgactg ccgcaccgcc
      421 gaagtggctt gcggataaat atgacatcgt gcagagggac aaatacggca gaaaaaagga
      481 ttggggttca agacgtgaag gctgcggaaa caatccgagc tacatagaaa agtcaaaaat
      541 cataaccgaa aaaatggctg aacattacaa ggacaatcca aacgtcatag catggcagat
      601 tgataatgag ttcggctgtc acgtaagtac aagatgttat tgcgagcatt gtcgaaaggc
      661 ttttgcaaaa tggcttgagg aaaggtatca gacaatagag aacctaaacg aaaaatgggg
      721 gagcgtattc tggagcttaa actatgattc ttttgacgat ataatacttc cgaaatataa
      781 ttcgtgcgag ggtacatacg gcgatttgtg gtcgcacaat cccgcacttg acttggaatt
      841 cagacgtttt tcttctgaca cttgggtgaa ttatcagaaa atgcagattg atattttgag
      901 aaaatatacc gataatccga taactcataa tttgatggga catttttcgg atataaacgc
      961 gtatgatttg tcaaaagacc ttgattttgt atcgtgggac aattatcccg acaatcaatg
     1021 gggtacgtcc gaatatgaat atgtgtctat ggcacacgaa aatatgcgtg gtgcgaagaa
     1081 caaaaacttt gttgtaatgg aggaacaggc aggacctgcc ggttgggata ttttaggctc
     1141 aacgccacgc cccaatcagc ttagattatg gacatatcag gcaatcgcac acggctgcga
     1201 gggcgttgta tatttcagat tcagaacggc tcttttcggt atggagcaat attggtacgg
     1261 tgtacttgac catgacggtg tgccgagaag acgtttttat gaaattcaaa agacaggtca
     1321 cgaacttcaa aaacttgaaa aatatattgt cggctcaaaa aataagtatg atgcattgct
     1381 tgtgaagtcg tacgacaatg tatggggaca tgatataaaa cgtcacgcac aaaactataa
     1441 ttatgaaaat cacttgtatt cattttacaa ggcaaattgt gatttgaaca taaacacagc
     1501 cgtttcaaac ggcaaatatg aagattataa aatcgtgtat atgcctgcgt acaacatagt
     1561 cacagacgca gagacggaga aaataaagga atacgtcaaa aacggcggta cacttgtttt
     1621 gacgtttagg agcggtacgc gtgatgaata taacagagta agacctatgg cactgccggg
     1681 agtgttcaag gaaattgcgg gcattgaggc ggttgaattt gacgcaccga gaaaacctgt
     1741 aaaaatatac ggtgaagtaa gcggaagtgc ggaaatatgg tgtgatataa taaaacctga
     1801 taccgcaaaa accatatgca catacggaag tgaatactat aagggtgaaa gtgcagtgac
     1861 agtaaacgaa tacggcaagg gcagagttta ttatgtaggc tgtgatttgg acaatgacgc
     1921 aatgaaagag cttgtaagaa ttatagcaaa gtcggcgaat gtggaaacag ttgatacacc
     1981 gaacggtgtg gaggttgtta agcgagatga ctgcacaatt attttaaatc acaatgaaaa
     2041 tgaagttgat acaaaaatta aaggagtgtc gctgtttgat ggtttgaaat ttgacggagt
     2101 attggacggc tacggagtac aatttttgga gggcagtata tgatatacaa atttaaagaa
     2161 tttaaagatg aaccgctttt gataaatcat ttgaatatgg gcggtaaaaa tccgaaaggt
     2221 gaagaaatta acgtcacaag cagatatttc acacgaaacg gaaaaccttg gataggcgtt
     2281 atgggcgagt tccatttttc gagatacggc agagagaatt ggcacagaga acttgcaaaa
     2341 atgaaagccg gcggtataac tattgtttca acgtatctgt tttggatata ccacgaagaa
     2401 attgagggta aaatggattt tggcggtgac aatgacatca gagcgtttat caaagaatgt
     2461 aaagatgtcg gcttggacgt tgtaattcgt ataggccctt gggcacacgg tgagtgcaga
     2521 aacggcggtt tccccgattg gcttttgaaa aaggattata aacttcgtga caacaacgaa
     2581 gaatatcttg cggttgttaa gaagtggtat cagagcatat ataacgaagt gaaaggactg
     2641 ttctacaagg acggcggaaa cataattgcg gtacagattg aaaatgaatt tgtcgacaac
     2701 gccgagcatt tggcaaaact taaagaaata gccgtagaat gtggatttat tgcacctata
     2761 tacacagtta cgggctggaa cagtgcatcg ggtgcgaaaa ttcccgttga tgaggttgtg
     2821 cctgtattcg gcggttattg tgaggcaccg tgggaaaatc atatgaacag actgtcaccg
     2881 tcaccgcact atttctttaa cagaatgaga aacgattccg caatcggaac ggaccttatc
     2941 gcaaaaactc agagtgacgg ctggcagctg ccgtatgagc gttatccgtt cgcaacgtgt
     3001 gagcttggcg gcggtataga ggttacacat caccgcagac cgattataaa gcctatggat
     3061 atatatgcgg tgtcgcttgt aaagctcggt gacggaaaca atcttgtagg ctattatatg
     3121 tatcacggcg gtacgaacaa aataggtgag ctgtcaactt ttaacgaaac aaaggcgaca
     3181 ggctatccga atgattatcc gatactttcg tatgattttc aagcaccgtt gtcggagtac
     3241 ggagaggtca gagaacagta cggattgctt aatatgctgc atatgtttgt aaacgatttc
     3301 ggcgaagaat ttgcaccgat gattgcggtt gattctggaa atacggtggc ggcagacgat
     3361 acaaattctt tgcgttacgg tatgagaacg aacggcaata gtgggtttgt gtttgtaaat
     3421 cattatcaga gattgaccga acttgcagat attgaaaatg cagtgataag tgcgggaaat
     3481 gttgagtttc cgccgattga cgtaaagggt gaagtgagtt tctttatgcc gtttaatatg
     3541 aaaatgggcg acagtgtgct tgaatatgcg acagcacagc cgttatgcaa gtacgatgat
     3601 acatatttct ttgcggaaat accgaatatc aaagcggaat acaaattcag caaaggcagt
     3661 gcaaatattg ttacagtgcc gtttgaaaat gctaagtata tgcgtaaatt aaacggaacg
     3721 gtgtatattg gcggaggttg taatttgtat gaggaaaacg gacaaataca ctctgttgag
     3781 gacggcgaat atatttgtca aaaatggaac ggcagtgaat ttgaaacact taaaatcggt
     3841 cagtcggcaa agcaatcaaa tgttgaaatc acaggcgtgg aaaatgcacc gtttgagccg
     3901 aagtacaaag aagaattgtg cattggcgga gaaagagaac tgacatggaa gaaaatcaat
     3961 gttgacggca gatacggatt tgccgagatt gactatgtcg gtgacgtggn nnnnnnnnng
     4021 cgcagattta tgcggatggt gaacttgttg cagatgatta ctattacggt aagacgtggc
     4081 gagtgccttg taaattgctt tacggcaaag aatgttatat ggtaatatcg gaaatgaaag
     4141 acgattttta taaggaattt taacaggaga gataaaaatg gaaatgacat tacgttggta
     4201 cggttcaaaa tttgataccg taacgttaaa gcaaatacga caaataccgg gcgttacggg
     4261 cgttataaca acgctttacg ataccgcacc gggtgaagta tggagcagag aaagaattaa
     4321 agaaatgaaa gatgaagtcg aaaaaagcgg acttcatgta agcggaattg aaagcgtaaa
     4381 tgttcatgac gcaattaaag tcggaacatc ggacagagat aagtacatag acaattatat
     4441 tgaaacgctt gaaaatctcg gaaaagagga cattcatctt gtgtgctata actttatgcc
     4501 cgtattcgac tggacaagaa gtgagcttgc aagaaaacgt ccagacggtt caaccgtgct
     4561 tgcatacaat caagatgata ttgataaaat cgttcccgag aagatgtttg aatctatttc
     4621 aaaggatatg aacggcactg ttatgccagg ttgggaacct gagagaatgg aaaaagtaaa
     4681 agagcttttt gaaatgtata aggatgttga cgatgaaaag ctttttgaaa atttgaagta
     4741 ttttcttgaa agaataatgc ctgtatgtaa taagtacgat ataaaaatgg caattcaccc
     4801 cgacgaccct gcttggagcg ttttcggttt gccgagaatt ataattaata agcagaatat
     4861 acttcgtatg atgaaaatgg ttgacgatgt gcataacggc gttactttct gttctggttc
     4921 gtacggaaca aaccttgaaa acgatttgcc cgatatgatt cgttcattaa agggaagaat
     4981 acattttgcg cacgtcagaa atcttaaatt taattcaccg accgattttg aggaggcggc
     5041 acacttgtcg tccgacggtt cgtttgatat gtacgaaata atgaaagcac tttacgaaat
     5101 cggatttgac ggtcctatcc gtcccgacca cggaagaatg atatgggacg aagtggctat
     5161 gccgg
//
LOCUS       DAWACS010000051         5114 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1817_length_5114_cov_2.166634, whole genome shotgun sequence.
ACCESSION   DAWACS010000051 DAWACS010000000
VERSION     DAWACS010000051.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1817_length_5114_cov_2.166634"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1433 a         1312 c          773 g         1596 t
ORIGIN      
        1 ccttagaaac agtgccaaaa cacttaaaga cagccttaaa tcaatcggtt tttaaatcat
       61 aaataattgc attacagatt gcggcggcca catttgagcc gccttttctg cctcttgcta
      121 ctatatacgg cacacccgat gtcataatca attcttttgc ctgtacaacg ttcacaaagc
      181 cgacaggtac accgattatc gcaatcggat taatttgacc gttctttatg agtttatcaa
      241 gttctataag tgcggtcggt gcatttccca cagcaaaaat cacgtctttt tcaagttttg
      301 acgctttttc catactcgca acggctcttg tagtgccgtt ttcctttgcg gcttttgcaa
      361 cgtcctcgtc ggacataaaa caatgtacct ctccgccata taacgcaaga cgttttttgt
      421 ttattcccga acgtgccatt tgcgtatcgg ttacaatatc cgcaccgttc tttatcgcgg
      481 tctttataat ctcaaccacg ttttccgaaa agcacaaata gtcaagataa tcaaaatcgg
      541 cggttgtgtg aataacacgt ttaacaatcg gtttaatcgt tttttctata tcgtgcggaa
      601 gttcgctctc tatgatttca aaactcctct tttcgatttc tgaaggcaat acgttttcaa
      661 attccattaa tattctatcc cctctcttgc aacaattccc ttgtcatatg ggtgctttat
      721 ctttttaatt tcagaaatat agtcggctct ttcacaaaac cattcatcag ggtttctgcc
      781 cgtcataaca agctcgccgc cgtaattttc aacaatattt ctcacactcg ccttatcaac
      841 caaatcataa ttatatgacg caaaaatctc gtcaagcact atcatatcaa attcattcat
      901 attatcaagt gcatactgta aattttcgtt atggcatttc acgatatttt ctttgtcact
      961 gtcggacata cttctgaaaa agccgtagtt tttgtcacag cgcataacct ttatattctc
     1021 acacttaccc aaaacttcaa gttcccccgt atgcgagcct tttaaaaact gcataaaaag
     1081 cactttttta ccgtaaccgc cgcaacgcac cgcaagaccg attgacgcgg tcgttttgcc
     1141 tttgccgtca ccgcaatata tgtgtatcat tttctacacc tctttgttaa taattttgta
     1201 tatcaagtcc atatcaagac tttcacgcac catatcggca agcctgtcat attcacgttc
     1261 tttcagttcc gccatatcaa tgctcttgat ttttgtactg tctataccct ttttgtcaca
     1321 caaacacttg acaattttat cggcacactt atcaaatatg ccgtgtacat aacttccgtg
     1381 aacgtcacct tgtgaaattc cgtcttgctt gccgtttgac agcttcgtca tacaagggac
     1441 actaaattca cttttaccca tatgaatttc atatccctca aattcagtgc cgttaaggct
     1501 ttttagtgtc cccgtcatgt tttcaaaaac accgtttact atcgttcttg tcttttcggt
     1561 tttaaactcc gtttccatag gcaaaagtcc cataccgcga acacttccgc cgttttccac
     1621 accgtctgta tcggtgattt tttcaccaag catttgataa ccgccacata taccgaaaat
     1681 agggcattta ctttttttaa ctgccgtttc aataccgttt tccctcatcc ataaaaggtc
     1741 ggcaacagta tttttacttc ccggaagtat aatcatatcg ggattaccga tttcactcac
     1801 attattaaca taccttaccg acacaccgtc tatgcattca aacacattaa aatcggtaaa
     1861 gtttgaaatt cgcggaaaac gtacaaccgc aatatcaatc acatttacag tcttattttc
     1921 aaaacgttcc gacaggctgt cctcatcgtc aatcataaaa tgaccgtacg gaacaactcc
     1981 gacaacaggt acattcgtaa gattttcaat catatcaagt ccgggacgca agatttccac
     2041 gtcaccgcga aatttattta taattgtccc cttaattctc gacttttcct catcatcaag
     2101 caacataaca gttccgtaaa gctgtgcaaa aacaccgccc ctgtcaatgt caccgacaag
     2161 cagaacaggt gcgtcagcca ttttcgccat gcccatattg acaatgtcgt cctgtttcaa
     2221 atttatttcc gcaggacttc ccgccccctc aataacgatt atatcgtaca tttccgacag
     2281 tttgttatac gctttcataa tgtcgggtac aagtttcttc ttatatttaa agtagtccac
     2341 cgcattcata tcgccctgaa cttcaccgtt tacaataact tgtgagcctt tatcgttggt
     2401 aggtttaagt aaaatcggat tcatcaaaac agacggcttt acgcctgccg cctctgcctg
     2461 tacgacctgt gcacgcccca tttcaagtcc ctcgtcagtg atatacgaat tgagcgccat
     2521 attctgcgac ttaaacggcg cagccttaaa gccgtcctgt ttaaaaattc tgcacaatgc
     2581 cgccgcaata atgctttttc ccgcattgga cattgttccc tgtatcataa tcgctttagc
     2641 cacatatatc acccaactct cttataaatt ccgcgttttc atcgtgtttt ttaactgcaa
     2701 ttctgtaaaa gccttcatca aggcggttaa aatttccgca gttccttatc agtatttctc
     2761 gctttaaaag tgacttgtca agtccgacgt tgcctttaaa taaaataaaa ttcacgtccg
     2821 aattaaatac tttaaagccc aaatttttaa gctcacccat taaaaattca cgttcttctc
     2881 ttataatttt tttacctttt tcaagataat cattatcatt aatcgccgta attccgccga
     2941 tttgtgccgc cgccgacaca ttccacatcg gcagctgcct ttgcacgtca catacaaaat
     3001 cagtatcggc aatcatataa ccgagcctta atcctgccat tgcgtaagtt ttcgtaaaag
     3061 ccttgataac aggtactttt tctttcatcg aatatccgtc agacaagtcc ataaagcact
     3121 cgtcaaacac acacacgatt ttcctttcac cgcaaacctc cgcaatatgt tgagcctttt
     3181 ttttatctgc aatttttccg acaggattat tcggattgca tatgaaaacc atatcaatat
     3241 catcaagata atcaataaaa tcatcttgca gttcaaaatc attttcttct ttcaaataat
     3301 aatagcaaat ttgcgaaccc acgcttttaa gcgtcctctc gtactccgaa aatgtaggtg
     3361 ccaacaaaag tgtttttttc ggcataaccg cacgaacgac cgcaaaaatc atttccgccg
     3421 caccgtttga gcaaagcaca ttatctacgc cgacctgctc tttttccgca atggcatttc
     3481 ttaaatttac acacattgcg tcaggataaa taccgaactg ttcgacattt tccgcaatcg
     3541 ctttttttac gttttcactc attccgagcg gattggtatt caccgaaaaa tcaagacgca
     3601 ccttatttct gtaaatgtca ccgccatgct catataccat atacaacacc taccgccact
     3661 ttcaatccga caaacagcac cgccgaaatt acggacgtca tatacataag tttaatcgaa
     3721 tctgcaatat tatcatattt aatcggctta atgctatcgc ctataaacgg ctttttgtac
     3781 agcttgccga aataatacgc gtcacctgca agctgaattt ccagcgcacc agccacgacc
     3841 gactctgtct gagccgagtt cggacttgcg tgattgcgtg aatcgcgttt aaaaattctg
     3901 tacgcattgc gtgaattgca acccataatt tttgacgcaa aaatcatcaa atatgccgaa
     3961 attcttgacg gtatataatt tgcaatatcg tcaagctttg cggcaaatct gccgaaattt
     4021 atgtacctgt cgttcttgta accgaccatt gaatccatcg tgttaatcgc cttgtaaacg
     4081 caacccaaca caccgccgcc gattgccata tatatgagcg gtgcaataac tccgtcagac
     4141 gtattttccg caacggtttc aacagccgct ttcgttatcc caatgtcgtc aagactttcc
     4201 gtatcacgtc cgacaatcat agaaacgctt tttcgtgcgc cgtcaacgtc accgtttttg
     4261 agcggtttat acacactcat acctgcttgt ttcaatgact tcgccgcaag cataaaatag
     4321 catataacgc tttcaatcgc aataccgaaa aagtaattta atctgtacgc aaaaaacaaa
     4381 atcgcaaacg gtacaccgcc gcatataata agcacgaata tcgccataac cgcaccgccg
     4441 agcctttcgt ttttcggaaa aattcttctc ataacctttt ccaaaaacga aatcaaataa
     4501 cctataagtc gaattgggtg cagtttaaaa tccgggtcgc cgaatattgc gtcaagcaca
     4561 aaacctatac taaccgcagc cgattgggct ataatcatca actatctctc cgtttctcat
     4621 ttcaaattca tatccgcagc cgtttttgac ctgataatca taaaaattac cgccaaaaaa
     4681 tttctgcata atcgccataa tacttccgcc gtgaattaca aacgccgtgt cacactccgt
     4741 aaggctctca aaaaagccgt ctttgcagcg atttttaaaa tcttcgtgtg cctcgccgtt
     4801 cggaaacggc aattcaccgt tgctgtcaag ccacttttga taatcgggat tatcctttaa
     4861 atcctcgtat gatttattct caaaatcgcc gaaatcacat tccttgagct tatcgcaaat
     4921 ttcaatatcc acattcggat atatatattc cgccgtttga atacacctct ttaacggact
     4981 tgcgacaatc ctctcacact taggataatc gcctttcaga cagtcggtat ttatcaaatc
     5041 ttcgtccgtt gttccgatat atcttcgctt taaatttccc tccgtcatac cgttacgtat
     5101 aagtattatc ttca
//
LOCUS       DAWACS010000052         5075 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1834_length_5075_cov_2.701992, whole genome shotgun sequence.
ACCESSION   DAWACS010000052 DAWACS010000000
VERSION     DAWACS010000052.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1834_length_5075_cov_2.701992"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    225..234
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1405 a         1101 c          881 g         1678 t
ORIGIN      
        1 ggctcgtcct tttgcgtaag tttgccgtaa gccttcgcat acgtctttgc aaggtacttt
       61 gtcgaaagca agtatccgtc ctgtattgaa tttcccaaac gatgatttac atagtcgaat
      121 atatcaagca tatgacattg atgtccgttc tcctcgaaat attccataat agccttagcc
      181 gtagaatgat gtccgtaacc ggcttttacg gttaacaaca acacnnnnnn nnnnaaataa
      241 atttttcaac cttattgact ttatgaattt aatagactat aatgtcataa gttattaaaa
      301 cgaggggcgg tgtattgtga aatgataagc gatatgacta aaggaaatcc gttaaaattg
      361 ataatttctt tttcagttcc gatgctgatc ggaaatatct ttcaacaggt gtataacttt
      421 gttgacgcgg cgattgtcgg taagtttgtg ggggctgaat ctcttgcagc ggtaggttcg
      481 acagggacga ttgtcggagt tatgatttgt cttatgatgg gacttacgag cggtgctgga
      541 attattatag cgcagtgttt cggttcaagg aattttgacg aactgagaaa aacagttacg
      601 ggacttattt acatagtggg aattttggcg gtggttacgt cagttgccgg agttgtgttt
      661 tcacggccga ttttatcgtt tctaaacaca cccgataatg taattgatga ggcggttgca
      721 tacataaacg taatattcgc attcacaatc ggtacggcta tgtataacgc gtcaagcgca
      781 atactcagaa gtcttggcga cagccgtaca ccgctttacg cacttatact ttcttctata
      841 ttaaatgtaa ttttcgactt gttgttcgtt gttgtgttta aaacaggcgt tgtcggtgcg
      901 gcggttgcaa ccgttatcgc acagattatc agtgcgattt attgtattat acatatggta
      961 agacaccgca gacagcttaa tcttgagggc ataaacttcc aaacaacaaa acgtgcaatc
     1021 aagcggattt tccaaacagg aataccgtcg gcacttgaaa gctgtctgat ttcactcggc
     1081 acaatgagtg tgcaacgtct tgtaaactca ttcggtgcaa tgactatggc aggctatact
     1141 gccgccgtaa aaatcgactc aatcgcaatc gcaccgattg tatcggtcgg ctcggcattg
     1201 tcggttttct cgggtcaaaa tatcggtgca aatcaaatgg acagaataaa aaagggtatg
     1261 tatcaaacac ttgcgtcact tatcggtata tgtatagtga ttgctgccgg aattgtattg
     1321 ttccgtaatc agttacttgg cttgttcctt gacaaaacac aagccgcaga ggctatcgca
     1381 ataggaagca agtaccttac tatcgtatgt gttgcgtata tagttgcggc ggttatgaga
     1441 acatatttga acgttcttcg cggtgcgggt gatgttaata cctccgcagt cgcaggtata
     1501 cttgaactta tcggaagaat tatttttgca tatatacttg ttcgcccgct cggctcaaca
     1561 ggtatttgga ttgctactcc gctcgcctgg gcaatgggtg cgtcagtacc tgttatccga
     1621 tactattcgg gtaagtggat taataaaaag ttggtataaa aaaatggcac tttcgtgcca
     1681 tttttttata cttattcttc tactaatttt actgttttct tttccgaaat aggttgtaat
     1741 tgtgatgaca atacaaatat attcacgttc ttggcattat cctttttgtc aagagaaatt
     1801 tttgtatcgt ctttcatcga aagcggtact ctgtcaattc caatcataac gccgttttca
     1861 tcatacattg ccgcatatac atagcagtcg ccgtacttca tttgtgcgga tatatcaaaa
     1921 tcatatgatg tgtcgttttc tgtttttgta acttctaccg ttgtcatagt catatccgta
     1981 ggtattccgt tataatggat tgttgcgttt gtaagatatg tatttccgac caacacaatc
     2041 ttactccact cttcctccgt acctgcgtaa tacacattgg taatactata acaatcacta
     2101 aaagcatttt ttgcaatttt atttatattt ttacccatcg ttacagtggt taagtttgta
     2161 cagaaggcaa atgcactcat ccctatcgac ttaaccttat tcggtatgtc aatacttgtc
     2221 aaactttcac attcgtaaaa catattttct tctattttgt cgatattttc agacagcttt
     2281 atgctttcca acttgttgca ccagctaaaa gcttcctctc ctatgctttt aacattatcc
     2341 gtcattgtaa cattgactac tccgctcgaa taaaaagcac cgacgcctat acgttcaaca
     2401 ctttcaggta tgtttatatt tgctaaattg ctgcaagaat taaatgcatt gtcatctatc
     2461 accgtaaccg gcaagccttc aatttcactc ggtatagtag cttcgctgac agatttgtca
     2521 cacttaacaa taaccactga ttttccgtcg gtactaaaat tgtaagtaaa cggctcgatt
     2581 gttttattat aatgaattgt tgcgtttttc aacgcgccgt tgccgtcacc tgtgctgttt
     2641 tcttctatat caattttatt ccattgtgtt tcggtaccaa aatagtatat atcttttaaa
     2701 ctgttacaat cataaaacgc atactgttca atcaaatcaa cacttttagg tattgttata
     2761 ctttccagac ttgcacaacc gctgaaagca gaagaatata ttgtatctac atcacttgga
     2821 atttttacac tctttaggct tgtacaattt gaaaatgtcg aaccctgaat ccaacttaga
     2881 tcctcagata ataccacatc agtcaaattt gtacaatctg aaaatacaga actgcccata
     2941 tcataaacat tatctgtaag agaaaccgaa gtcaagtttg tacaaccata aaatgcctgt
     3001 gcctgtatat atgtcacgct ttcaggaact gtatagcttg tatcgctttt ttccgcagga
     3061 tatgcaacaa gttctgattt atctttatta aaaagcactc cgttgtcaga agtgtaatat
     3121 aggttgtttt catcaacgtt tatcgcttcc aaattaacgc tgttactgaa tgcacccgct
     3181 gctattgatg ttgtttgttg cggaacattt atagtattaa cattttgtgt atacttaaaa
     3241 gcacaaccgt caattttcga aacacttgac ggaattgtat agcttacacc actttttgca
     3301 gcaggatatt gaataagttc tgttttactc ttgttaaaaa gtactccgtc ctttgaaata
     3361 taatttttgt tttcagaatc gacaactata ttttcaaggc ttgtacagcc caaaaatgta
     3421 tcgtacccta ttgaagttac ggttgacggg attgttactt tcttcaaatc cacacattta
     3481 aaaaacgcat cgccatcaat ctcagttacc gttgctccct caatttcact tggtatattt
     3541 acttccgcca cttccttggc acattttgaa atcgtaaccg tattatcttc atttacccaa
     3601 tattccaaat agttctcata cgtttccgca cttgccgtca tcggcacaaa tgcacacagc
     3661 attgacagtg ccaaaatcat acttaaaatt ttctctctca taaaataatt gcctcccaca
     3721 tataaatttt tacatatata gcttatcata tcttggtagg aattgcaata tatttttcaa
     3781 atattgtctt ttttagtttc ttatcaacct tattgtcgca acggtcatat cgtcgattac
     3841 tgcgtcacgg ctttttttga ctgcattttc aattacggat tgtgccattt cgtccatagt
     3901 gtcaaacggc atttttattt cttttttcag ccaatctgta cggacagtac caaatcccgc
     3961 ttcgcttatt ccgtccgaca tcattattat tatatcgccg tcatcaaggc gtttggcttg
     4021 tccctgtacc tttatatcag gcagcatacc gacaggcaac gatacagaaa acaccgtttc
     4081 aacattcgga ccgtgaagta caatactctc cgaaccgccg attttgaaaa actcacatat
     4141 acctgtcata aggtctatac atagcaggtc cacggtagaa aaacactcat agtcaagttt
     4201 taaacagagt gccgaattta tcatatctat agacgttttc acgccaaacc ccgactgcaa
     4261 aaattctctc aacaatttca gtgttatatg actctcaaac attgcccttt taccgctgcc
     4321 cataccgtcc gacaaaatca catactgttt ataatcctcc gtcacaaacg tatctatact
     4381 gtcgcccgac acttcgctga aatctcggct tatctgtctt atcgcaatat ctgcggtaaa
     4441 ttttgcgcgt gacacaaatt tcataagtcc gttcgcactg tcacattcat agcccatagg
     4501 tgtttcgaca acgctttcaa gcacgttttc aatcttcccg acttccgtac ccttagacgt
     4561 tccgatatat acctccattt ttccgtgact gctttcaata acgcttattt caaatgccat
     4621 aattccgact ttgtcaagct cggcaataag tgtttcctcc aaatcctctc tgaacgtaaa
     4681 gccggaacat atattttccg ccattgtgtc cataaggctt gacatttctt tatactgtct
     4741 tgcaaccaag tcacggcttg taaccgcctc acccgtccgt acaaggtttt tcttatacag
     4801 ttcatagacg tgattaaact ccacaacgaa caaatcaggt cgtaagcatt tatccttaaa
     4861 gctgttcggt actgatgtaa actccaatat cccccttgtt tcaatcgtat caagcaacag
     4921 cattatactg cggtatgtgc gtgtaaagtc ggactgccag catttaaccg cattcggaca
     4981 cccctcgcac actctgtccg caacctcatc aaaaagtgag gcaacatctt tattatatga
     5041 tttcagtctt ttctccgaag catttgaaaa acact
//
LOCUS       DAWACS010000053         5073 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1837_length_5073_cov_2.420486, whole genome shotgun sequence.
ACCESSION   DAWACS010000053 DAWACS010000000
VERSION     DAWACS010000053.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1837_length_5073_cov_2.420486"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4062..4071
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1626 a         1133 c          886 g         1418 t
ORIGIN      
        1 gcaacatctt tattatatga tttcagtctt ttctccgaag catttgaaaa acactcctcc
       61 aagctcttaa atgcctttgc cgatttttca agctgcatag acaaatactc tttaactctt
      121 acgtccgcac tcaccgtttc aagctttagc gaccttgcaa aaaacgattt tatataccct
      181 tgaactttat tcggtgtcag tacaaaaagc accgccccta tcgctgtttc gataatcgtc
      241 acaggcaaag acgatgcact tcccgcatac aaaagcgcca ccgcactgcc gcccaagaaa
      301 cccaacgcca ctccgaaacg tccgaacgat ttaagcaaat ttccgaacaa tgcacttata
      361 ccgaaaatac ccatagtcac aaccgccgac ggtgacgaca ttgccgacat aaaccctata
      421 caaagtcccc cgctgcccgc cgccgcaata ccgccgtgaa gtgcaatgca aagtaccgca
      481 tatacggaaa ctatattggc aagactgata ttatacggaa acacaattcc cgaaagtccc
      541 gttataaaaa cacccacgct tacagaaata ctgataagct cgtcctgcgc ggtctgcgtg
      601 cgttttttgc ggtttgcaat caatccgtgt gcttttttga atattatata catcaatgaa
      661 gtgactatcg actcaacaaa aagcataagt atatcatatg ctccgacata tgtatatata
      721 agaaaaacaa atccgccgac cataaccgca ccgcccacgc acgccgcgtc aagcacaaga
      781 ttttccttat ttctgaaacg tgtatatatc caaaacaaca gtgccgcaac aagatatttt
      841 gtaagcaccg cactgcctgc cgacgtcata agtgcaatcg aaagcactgt tataccgaga
      901 tacgcaatac ttttatcaaa gcaagaagca aaaaaagcca ctccgaacgg aaaaagtccc
      961 aaaacagacg ctcgtcctgc caaaagcaat agcatactca ttcccacatc tctcacagac
     1021 ggtgctcgga gcatttctgt attccgttgt ctttttaccg ctggctgact catttttaat
     1081 tcctcctaca agtgcaatat aggttattat accacttgtt tatggataaa tatgtaaaat
     1141 cctgttgtcg atatataaaa atatttcgac atcatttttt gacaaattgc gtttgcatat
     1201 gatatactac atacatactt tttatattga gaggattaga ttatgaaaaa aattttgatt
     1261 ttttccgata ctcacggcga tattaaccgc tgtttagata taatcgaaaa agccgacagt
     1321 gtaagtgcga ttttccacgc aggcgattac actggtgacg cggaagattt ggagtcgata
     1381 tacccaaaca tacctatata ttatgtaaaa ggcaacaacg actacttttc aaaagcgccg
     1441 tcacatatgc ttgttacaat agacggtgtt aagatattta ttacgcacgg tcacgaacag
     1501 aatgtaaaat acgaatatga atttatgact ctccgcaagg ctgccgaaaa atatgatccc
     1561 gatttaataa tattcggtca cacgcacatt ccttataccg attacaaagg caaagccaca
     1621 cttttaaacc cgggaagtat acgttataca ggcacatatg cagaggctga aatcgaaaac
     1681 ggtaaactaa aaacaaaaat catagaggtt tgatgtacaa attatacaaa aatgtcacgt
     1741 tattttatat atccccgtta tgcgtgacat tattaatgaa ttatttgtaa aaatgtcacg
     1801 tcttgtcacg caatatgaca attttaaacg atttatacct ttttaatata gaagatttat
     1861 cctttatatt gcaattttta cagtaataat ttatactatt attgaactta attgagttca
     1921 ttaaacgaga aaagacggtg cattccccaa tgtgcaccgt cttttttatt atagattttt
     1981 tgttacctct tttaaatatt ccgcaaattc atctttcaaa tcatctcttg aaagtgcaaa
     2041 atcgactgtt gcctgtaaaa atccctgttt gcttcctata tcatatcttc tgccctcaaa
     2101 gtcatatgcg tacatatctt cattctttgc aagcattaca agtgcgtcgg taagctgtat
     2161 ttcgccgcct gcacccttag gtgttttttc aaggcaatcg aatattgcag gtgtgattac
     2221 atatctgccg agtaccgcaa cgtctgacgg tgcgtcctct tttttcggct tttcgaccat
     2281 tcctttcagc ttatacattc tgccttcaac attatcgcag tccacaatac cgtactttga
     2341 aacttgatcc attccgacct tttgcactcc aagcactgtc gcattatatt tgtcaaactg
     2401 ttcgataagc tgactcaaac acggcttttc cgccacaacc acatcatcgc cgagcattac
     2461 cgcaaaaggc tcgtttccga caaagctttt cgcacaaagt acagcgtgtc caagaccttt
     2521 ctgttccttt tgacgtacaa actgaacatt tattttatcc gacacttcac gaacagcatt
     2581 aagcatttcc tgcttgccgt cacgttcaag tatcgtttca agctcatatg ccttatcaaa
     2641 atgatttaca agtgcgtcct taccgcgtga tgtaattata agcaaatcct caatgcccga
     2701 tgcgattgcc tcctcaacaa tatactgaat tgtcggctta tcgactatcg gcagcatttc
     2761 tttcggcact gccttagtcg ccggaagaaa tcttgttccg aaccctgccg ccggtattac
     2821 cgcttttgtt actttcatat tttctcctcc ctcaatttct aattttatga ttatctatta
     2881 ttataccaca tattttccat aattgtgata taaaaatcag ttaatttatt tcaaatttca
     2941 acacttgata ttgtttcaaa tctgcggtat aatattttta tatatataat tacagattaa
     3001 ggagaaaaaa cagaatgttt ataagagata tatttgctca aaaaaagccg gtgctttcat
     3061 ttgaagtatt tccgccgaaa aaggacagcg gacttgacaa tgtaatttca gcagttgacg
     3121 agctttcaaa aatgcctatt gactatatga gcgttacata cggtgcaggc ggcagcaatt
     3181 caaaaaatac cgcatacatc gcctcacatt taaagaataa tctttctatt ccgtcacttg
     3241 cacatcttac ttgcgttgca aactcaaagg acgatattaa ggtaaaatta caggaatata
     3301 aagaccttgg aattgaaaat atccttgcac ttcgcggtga caagccgaaa gattatgacg
     3361 gtgaaatatt cccgggttat cactatgcaa acgaacttgt agaggcaatc aaagatttcg
     3421 gcgatttttg tgtcggcggt gcttgttacc ctgagtgtca ccctgattct ccgagccttg
     3481 acgccgatat tgaaaattta aaagtaaaag ttgacgcagg tgttgatttt cttgttactc
     3541 aaatgttttt tgacaacgac gaattttaca aattccgcga taaacttgca aagaaaggta
     3601 taaacaaacc tatcacagcc ggaataatgc ctcttacaaa cgcaggtcaa atacagcgta
     3661 tggcaattct ttcaggcggt gcgtctatgc cgtcaaaatt cttgcgtatg atttcaaaat
     3721 accaagacaa tccggaggct ttaaaacaag caggtattgc gtatgcggtt gatcaaatta
     3781 tcgacctgct ttcaaacgac attgacggta ttcatcttta cacaatgaac aagcccgaaa
     3841 ccgcacagaa aatcgtaaat gttataaaca acctatttgt ataattcgca ttttgcaagg
     3901 agaacttatg aacggaaaac tatatttatg tgccacaccg ataggcaatc tcggtgatgt
     3961 gtccgcgcgt tgtcttgggg catttaacaa tgccgattta attgccgcag aagatacaag
     4021 gcgtactctt cagcttttaa atcactttga aatatcaaag cnnnnnnnnn ncaaagccgc
     4081 tgacgagcta ccacgaacac aacaagcacg aaaaaggcga atatattata tcacttttaa
     4141 aaaacggtaa aaacattgta cttgtatcgg acgcaggtac tcccgccatt tcagatcccg
     4201 gtgaggactt agttaagcta tgtatcgaaa acgatattga cgttacctca atccccggac
     4261 ctgttgcagg tataaacgca ctgatactgt cgggacttcc gacaagacga tttgcatttg
     4321 agggatttct ttcggtgaac aagcgtcaca gaaaagaaca tcttgaactt gtgaaaaacg
     4381 acactcacac actcattttt tacgaggcac cgcacaaatt aaaaaacaca ctttccgata
     4441 tgcaaaagac attcggcggt gacagaaaaa tcgctcttgt gcgtgagctt acaaaaattc
     4501 acgaagaagt gaaaagatgc acaatcgacg aggcaatcgc atattattcg gtgaatatgt
     4561 acttgtagtc gagggtgcaa aagaagtcga agaaacggaa gaaaacgaat gggaaaacat
     4621 ttcaattaaa gaacacgttg acaaatacat tgcggaaggt atgacttcaa aagacgcaat
     4681 aaaaaaagcc gccgcagaca gaaatcttcc gaaacgtgac gtatacaacg aatatcacag
     4741 aggtgaataa cgtgaaaaag agaacattat ttttaatagg cactgccgcc gcggcaggca
     4801 tatacagtgc aataacaggc aaaggtttat tcaacaaacc acgctttaaa gaacagcacg
     4861 aacgcgttgc aaactacgtt gaaactcact atccgaacgc ggtttacact cctatcgaaa
     4921 tgaccgcaaa cggttgggta acggtcataa gacgtattgc aagaccgaac ataatccttt
     4981 acatcacccg tgacggaaac ggcaattatg tctttaccga atcggaagtc cgaaatgcgt
     5041 aaccaaaaac aacgctttag catagtataa acc
//
LOCUS       DAWACS010000054         4963 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1882_length_4963_cov_2.185819, whole genome shotgun sequence.
ACCESSION   DAWACS010000054 DAWACS010000000
VERSION     DAWACS010000054.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4963)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1882_length_4963_cov_2.185819"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3669..3678
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1618 a          773 c         1175 g         1387 t
ORIGIN      
        1 gaaatgtact aatgaaaaac ggaataaaag ttgttgcaaa taattatgcg tcgtcaaatg
       61 ttgatatgcc gacaatgccg cttgatattt taagcgtgtt taataatgac aattctgttg
      121 ttgttttcga cgtcggcggt gacgatgacg gtgcattggc tttaggacag tataaacaat
      181 attttgataa atacggttat gagatgcatt ttattgttaa tacaaaacgt cctctgacaa
      241 gaacgaatga tgaattaatt gaaatcgcag agagaataga gagtgcatca cgtttgaaat
      301 ttacggatat atacaataat acgaatttat ccgaccaaac agacgaaaat actttatttt
      361 cgggatacga tgaaattaaa atgctgtcaa agaaaatggg tatagtcata acaaaaaatt
      421 gcggaattga taaggctctt tcaaacgttc cgacgaattt gaaggatact gtatttcgta
      481 tgaatatctg tattttaaga ccgtgggaaa tacagtgaaa acccaacaaa ggagttgatt
      541 tttttgaaga aagttatctt caacgaagaa cgttgtaaaa gttgtgaact ttgcgtaagt
      601 gtctgcccta agaaaattct tagaatatcg gacgataaaa tgaatgaaaa gggttacaaa
      661 cccgctgaaa tatttgaaca agataaatgt atcggttgtg cattttgtgc gacaatttgt
      721 cctgacgcgg ctatcacgat tgaggaggac tgacagatgg gtgaaaaggt tttaatgaaa
      781 ggtaatgagg caataggaga ggcggcaatt cgttcgggct gtcgtcactt tttcggttat
      841 ccgataacac cgcagacgga actttcggca tatatggcaa agaaaatgcc gaaaataaac
      901 ggagtatttc ttcaagcgga aagtgaagtt tcagcgataa atatggtata cggtgcggct
      961 gcggcaggaa tgagaacaat gacaagttca tcaagtccgg gaataagtct aaaggctgag
     1021 ggaataagct atcttgcggc aggagatttg ccgtgtgtga ttgtaaatat agttcgcgga
     1081 ggtccgggac tcggcgggat ccaaccggca cagtcagact atttccaagc tgtaaaaggc
     1141 ggcggacacg gtgactatca tttaattgtt ttagcgccta attccgtaca ggagcttgct
     1201 catttaacag ccgaagcatt taatatttcc gatgaataca gaatcccaac tatgattttg
     1261 ggtgacggta ctttgggaca aatgatggag cctgtcgact tagatgcaat acctgagccg
     1321 atttacgccg acaaaccttg ggcggcaaga ggaactgata ataaacgtaa gaaaaacgtc
     1381 gttgaaacac tttatcttga ggcgtcagag cttgaaaaga aagttttgga gcgtgaggaa
     1441 aagtataacg ttgtcaaaga aaatttgcag cgatatgagg cacatgatat tgatgacgcc
     1501 gatataatca tatcgtcata cggtattact tcaagagttg tcgaaacggc ggttaagtca
     1561 cttcgtgaag agggaataaa agtgggcgtt atacgtccga taacactttg gccgttccct
     1621 gaaaagattt ttaaaacggt taaaccgaaa gcgtttttgt ccgttgaaat gagtaccgga
     1681 caaatgattg aagatattaa gcttgcgtgt gaatgcagtg cacctgttta tttctgcgga
     1741 cgtacgggcg gtattatacc gacacccgat gaggttgcgg aaaaggttaa gagcataatg
     1801 aaggaggtac aataatatga aaactatatt taaaaagcca aattcattaa ccgatacagt
     1861 ttttcactat tgccccggct gttcgcacgg aattgtgcat agacttgtat gtgaagtcat
     1921 tgatgaactt gatattagcg gcaaaaccat tgcggttgag ccggtaggct gtgcggtaac
     1981 ggcacataat tattttgact ttgatgttgt acaagcggcg cacggcagag cacctgcagt
     2041 ggcaacgggt ataaaacgtt cactgccgga cagggttgtg ttcacatatc aaggtgacgg
     2101 cgaccttgcg tcaataggta ctgccgaaac agtacacagt gccgcaagag gcgagaatat
     2161 aaccgttata ttcatcaata atacaattta cggaatgaca ggcggtcaaa tggcaccaac
     2221 gtcacttccg ggacaagtta cgcagacatc accttacgga cgtgatccga aaatacaagg
     2281 taatcccgta agagtgtgcg aaatgcttgc cacacttgac ggaccgtcat atattgcgag
     2341 agtttcgacc gattccgtac cgcatataaa ggacgctaaa aaggctataa agaaagcgtt
     2401 tgaaaatcaa attaacggta agggattttc aatagtcgaa gtgctttcga cttgtccgac
     2461 aaattgggga atgtcacctg ttgaggctat gcagagagtc agagatgaaa tgattccgta
     2521 ttatccgctt ggtgttttta aagatattga ggacgtcgag gaggataaat aatgaaaaat
     2581 tgtgaaatga tacttgccgg attcggcggt caggggattt tgtttacagg aaaaatactt
     2641 gcgtttgcgg caatgcttaa agacaaaaaa ctttcttggc taccgtcata cggacctgaa
     2701 atgcgtggcg gtactgcaaa ttgtcacgtt ataatagatg atgagccgat tggctcgccg
     2761 attgttacaa gaccgaatat ccttgtggca atgaataaac cgtcacttga taaatttgaa
     2821 aatacggttt cggacggcgg atatgtgttt atagatacgt cgcttataga cagaagggtg
     2881 gaaagagagg acgtaaacga aatttgcgtg aatgctaccg aaacggctca taatataggt
     2941 aataagagtc ttgcaaatat ggttatgctc ggtgctgtgt taaagaaaac ggaattgttt
     3001 acaatcgatg aaattgaata cacaatgaag aaatctttgc cggaaaacaa gcataaactt
     3061 atagagatga atataaaagc cgtaaaagaa ggatataatt tagtatagag agattaaaag
     3121 gaggattttg gttatggcag cggtaaaaaa gaaaaaaggt cttatgtaca aaggtaagcc
     3181 gcttttaaga tgcgggaaca gaatttatta cggaaatttg gaagataagc ttattcttgc
     3241 tcttgacatc gtggaaagca gtaaaaagca agatgtggac gtatctacaa aagttaaggt
     3301 acagattatg gacaacacag gtgagcttgg caaaggtcag gtttatcgta agtgcgaaag
     3361 agaaaatctt tataaggcta ttgacatcgg tgaatggtgg ctgcaagacg ctatgaaaat
     3421 gatgggctaa ttaaatttaa gctgttgcat ttttatgtga cagcttattt gcatttataa
     3481 aatcataatc tgaaagggtg aaaaatgtga atttaaccaa agaaaatgta aagaaaattt
     3541 gcggaataat attgttcacg gttgtagtgt tggttatagt gcagaactac gaaaaggtcg
     3601 gaaacgctat aggacttttg tggggaataa tttttccgtt tgttttgggc ggaataatag
     3661 cttttatann nnnnnnnntt tctgcttgta atcggagtga ttgcgggtgt gatacttatt
     3721 gttgtgccgc aaataggcgg aactgtgaga gaacttacaa aatcggcaga agcatttttt
     3781 cctaaagtac agaaatgggc agaggatatg ttcagtaata atacgcaaat ccacgattgg
     3841 attgcaagtt tgcagttcaa taccgacaaa cttatggata ctgcgataaa tttcttacaa
     3901 aacggtgcgg gaaacgtttt aaacggtaca tttacggcgg caaaagctgt tgtatcggga
     3961 ataacggcat tttttatcgg cttaatattt gcgttctatc tgcttgcaaa aaaagaaaca
     4021 cttcaaagac aagtcaatat gtttatgcag gcggctttgc ctgaaaaaat agtgaataaa
     4081 ataacgtatg tcgcaaaatt gagcaatgaa actttttcaa actttataac gggacaatgc
     4141 cttgaggcgt tgattttggg tacaatgttc tttgttacat tgtcaataat acgattgccg
     4201 tatgcacttt tgattggagt tttaatcgct tttacggcac ttataccgat attcggtgcg
     4261 tttatcggct gtattgtggg tgcatttttg atgattatgg taagcccgat gaaagcactt
     4321 atatttgtaa ttgtgttcat tgtacttcag cagatagagg gtaatttaat ttatccgaaa
     4381 gttgtcggcg gttcggtcgg cttgccgtca ctttgggttt tggcggctgt tacaataggc
     4441 agcagcctta tgggcgtggc aggtatgctg ttctttatac cgtttacatc ggtgatatat
     4501 acgctcgtgc gagaatggac atataaacgt cttgaacaga aaaaaacaaa agaaaataac
     4561 aatgatgaga gtaatgaaag cagtgtgtaa agcactgctt tttgcgtaat cttttattat
     4621 aacatatttt aaaattgtgt gcaaataaac aattttaaaa ataaaaaatc gctgatttga
     4681 caagaattga aagtcgggtt aaaatagtga taacaaatta aagcaaagga gaaacacaat
     4741 gaagtttata cacttatccg atttgcacat aggaaaacgc gtgaatgagt tttctatgat
     4801 aaatgaccag aaatacatac ttgaacaaat acttaatata atcgcaaatg aagaaattaa
     4861 cgcggttatt gtatcaggcg atgtgtatga taaatcgata cctgcggtcg aggcggttac
     4921 ggtatttgac gattttctta cggagcttgt cacgaaagat ata
//
LOCUS       DAWACS010000055         4957 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1884_length_4957_cov_2.558548, whole genome shotgun sequence.
ACCESSION   DAWACS010000055 DAWACS010000000
VERSION     DAWACS010000055.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4957)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1884_length_4957_cov_2.558548"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1853..1862
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3421..3430
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1323 a         1051 c          929 g         1634 t
ORIGIN      
        1 taggacctgt tacatagtca ccgtattcag cagtattaga aattgagtat ctcataccct
       61 tgaaaccgct ttggtaaata aggtcaacga taagtttcat ttcgtggata cactcaaagt
      121 aagcgtttct tgggtcataa cctgcttcaa caagtgtttc aaaacctgct tgcataagag
      181 ctgtaacacc gccgcaaaga actgcctgtt caccgaatag gtctgtttct gtttctgttc
      241 tgaatgttgt ttcaagaaca ccggctcttg caccaccgat acccaaagcg taagcaagtg
      301 ctaaatcctg tgccttacct gttgcgtctt gttcaacagc gattagacaa ggaacaccct
      361 taccttcaag atattctgaa cgtactgtgt gaccaggacc cttaggagcg atcattgtaa
      421 cgtcaacgtc tgcaggagga acgattgttc cgaagtgaat gttgaaaccg tgagcaaaca
      481 taagcatatt gcccggttca agatttggtt cgatttcgtt cttgtacaag tcagcttgct
      541 tttcatcgtt gataagaatc atgatgatgt cagccttctt tgcagcctct gctgatgtat
      601 atactttcaa gccttgcttt tcagccttgc tccatgactt actaccttca taaagaccga
      661 tgattacatc gcagccagag tcctttaggt ttagtgcgtg agcgtgacct tgtgaaccgt
      721 agccgataat tgcgatagtc ttgcccttta gtagtccaag attacaatcg ctttcataaa
      781 atacttttgc ttcatggtct aatgtttttg ccattttgat tacctctttc ttaaatatgt
      841 attataaata ataattattt atctagtttt aatgtgtttg ccccgcgttc aagagcagtc
      901 ataccggtac gggcgatttc ttcaattccg tacggctcta caaggtcgat aaatgcgtta
      961 agctttgtat tatgacctgt caattcgata gtcaaagtgt taggtgaaat atcaactatc
     1021 tttgctctga atacgtttgt aatttcgata acttcacttc ttgtcttagg tgtaacctta
     1081 acttttacaa gtgaaagtcc tctctttacg atttcgctgt cacgaagtgt cttaattttg
     1141 ataacttcca taatctttga aagctgtttt gacacttgtt cgacaatttg tgcatcgccg
     1201 ttcacttcaa tagtgatacg agatacttgc gggtcttggg tagtacccac tgaaagtgaa
     1261 tgaatgttaa atccacgtct tgaaaacaag cctacaactc ttgaaagtac acccggattg
     1321 ttttcgacaa ggactgataa tgtatatctt tccatattac ttatcctcct ttttcggaag
     1381 agtagcctca ttaagagcat caccggtagg ttcaaaagga tttacgttta cgataagaag
     1441 atatgaaccg tcatgtgcca tcatcttatc tatcgcctct gaaacctcac tgttttcgtt
     1501 agctacgccg ctctcaaagc cgtatgcctt tgcaagcatt gcaaaatcag gttttccttc
     1561 tatttcaaca gcctgataat ttgatttata aagaagatac tgatgttcgt gtaccatacc
     1621 aagacggtgg ttttggaaca ataccatttt aacaggaata tcccattgag ccatagtacc
     1681 catttccggt aaatccattt ggaaactgcc gtcacccatt accgcgataa caggctttga
     1741 gccgtttgcg gcaacagacg caccgattgc ggcaggaagt ccgtaaccca ttgtaccgaa
     1801 tccgcctgaa gtcaaaagtg aacgtggtgt tttaaagttg aagttattgg cannnnnnnn
     1861 nnttgtcttt tccgaaagta cgtttaagaa atacttcgga tttacaaatc cgttatcctc
     1921 acataccggt ttatgtgtaa atctttgacg tagttcgtca gcttcgtcaa tccactcttt
     1981 gcttgtcttg taacctgaaa tttgcgatgt aagctgttca agaacatctt taatatcacc
     2041 gacaacagga atataaggct gagtgttttt gccgatttcg gcagggtcaa ggtcgatatg
     2101 tataagcttg ttgccttcct caagtccctt gacatttgca acagttctgt caccaagtct
     2161 tgaacccatt acgattacaa ggtctgcacg attgaaaaga agattagctc tcttaacacc
     2221 gtgtgaaccg atcataccgt agtatctcgg attgtccgac ggaagaatac ctatacccat
     2281 cattgttgaa acaacaggaa tatctgtttc ttttacaaat tttctgaatt catttgtagc
     2341 atcggatata acgacaccgc caccggcaag tataaccggc tttttagcac ctttgattgc
     2401 ctcggcaacc ttcttaacct gtgattcgtt agctttgccc attaacttgt agccacggat
     2461 attaacgtcc tcatcttcgt ccggataatc aaattccttt tttagaagat ccatagggaa
     2521 gtcgataagt acaggacccg gacggcctgt gtttgcaata tgaaatgcct catttacaac
     2581 acgcggaatg tcaagtccgt cttttataag gtagctgtgc tttgtaaaag gcaacgtagc
     2641 acctgtaatg tcaacttctt ggaaagcgtc tttaccgatt agctttgtag caacctgacc
     2701 tgtaagtgca accatcggaa tagaatccat atatgcggtt gcgatacctg ttataaggtt
     2761 tgtagcaccc ggacctgaag ttgcggtaca gacacctgcc tgtttcgtaa ctcttgcgta
     2821 gccggaagcc atatgagccg caccctgctc attacgagta aggatatgct ttatggaaga
     2881 aaaggacagc ttatctataa taggacagtt agccgcaccc ggataaccga atacaacttt
     2941 aacgccgttc ttttcaagtg ttttaacaac catttcagca ccggtcattc tcaaaaaaat
     3001 tccccctttg aaatttaatg taataaatat tggcacaccg attttctgtg ccgaaaaaaa
     3061 ccaccatatt tgatttgtct tgtggaaaat cgaattttct aaaagctaat gcttttttga
     3121 aaagatggtg ctgttttgtc cgtcatttta tgacggaata tgtacaaata tattatcgta
     3181 ctatttttat tttatcagcg aataagctaa ttgtcaactg tttttataca gaattgttac
     3241 tgttagatac ttatatttat gttaaaacaa attttttgta tattattgaa ttttgagatt
     3301 aaaatattca ttatcaggcg aattttatca aaaaaatcac tcataatttt tatattatga
     3361 gtgataaaaa attcagattt taaacaaaac cgttgcagtt ttgtctactt tatttagggg
     3421 nnnnnnnnnn tcttttaaat attcttttgt gtttctgccg accataattt caaacttgcc
     3481 tttttcaacg atatattcca aatctctcgt cacgattgca aggtctttta tgtttaattt
     3541 tatgtttacc gttgctgttt cgcctgattt tataaatacc ttttcaaaac cttttaattg
     3601 ttttgtcggt gtgaggacgg agcttatgac gtcgttgaca aacataagta cggtttcttt
     3661 gccgtccata ttgcctttgt ttgtaacatc aaccgatacg gttatttctt cctcattttt
     3721 tattgtgttt tgggataagc gtaggtttga atattcaaat tcggagtagg aaagtccgtc
     3781 accgaattcg tatacatagc ctttttccaa atcaacatac tgaccgccgt gccaaccctc
     3841 gtattgattg tagtagcaag gtgtttggga actgtgacgc gggaaagaaa ttgtcaattt
     3901 accgctcgga ttaaatttac cgccaatcat ttccgcaacc gccaaaccgg caaagtcacc
     3961 gccgttaaag tcctcgataa ttgcattgca gttttctttt aggtacgata tgcaaagtgg
     4021 tttgcagttt acaagtacgg ctattacggg ctttttcgtg tcaatcagac gtttgacaag
     4081 ttctgtttga taacccgata attcaaggtc ggctctgtcg cgatactcgc cgttttgagc
     4141 aagacagtcg ccgattacgc atacaaccac atcagccatt tcggcaattt ttaccgcgtc
     4201 atcaatataa ttttcttcac ctaaaatgtc acagcctttg ctgtataaaa tattctcttt
     4261 tccgaatact tcctccatac cgcgacgtat cgtgtagtag tcgcccatag gtgtaacgtt
     4321 ttctttcgga acagggtgtg aaaaatatgt ccagtcgccg taaatcgctc ttatgtcgtc
     4381 cgcattagga cctataacgg cgatggtttt gtttcctttc agcggcagtg taccgtcatt
     4441 tttaagtaaa accgcacttt cctgtgcaag tttgtggtta agttttttgt gttcattgca
     4501 gtttatgacg cttctgtcaa ctgtcttttt accgtcaaaa agacctattc tgaattttgc
     4561 tctaagtatt cttcttaccg cgtcgtcaat atattttatg tcaatttcat tgttctctat
     4621 aagttctgac acgcaatcgt aaaattcgtg cgtattcata ctcatatcgt tgcctgcctc
     4681 aagcgacagt tttgacgcgt cttttaagtt ttcggctatg aattgcttgg tgacaagatt
     4741 taagacattt tcgtaatcgc tcacaacaaa tccgtcaaaa ccgatttcgt ctttaagaat
     4801 ttctcttacc atacgcggat tggcgttaag cggaacggag tcaacagagc cgtaagcggt
     4861 cataaccgag ccgcaaccga cttttgcggc tttttcaaac ggcggtagga atacttctct
     4921 gatttttctt tcactgacct cactgtcgta cgcgtca
//
LOCUS       DAWACS010000056         4953 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1887_length_4953_cov_2.621886, whole genome shotgun sequence.
ACCESSION   DAWACS010000056 DAWACS010000000
VERSION     DAWACS010000056.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1887_length_4953_cov_2.621886"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1460 a         1025 c          944 g         1524 t
ORIGIN      
        1 gccaccccac agtcgactat gtcgacagct cccctcaatg ggagccatac aaaaagcctc
       61 tccttgagga gaggtgtcac gaagtgacgg agtggtggca aaagcataaa gcataagaaa
      121 gccaccccac agtcgactat gtcgacagct cccctcaatg ggagccatac aaaaaacctt
      181 tccttgagga gaggtgtaac gaagtgaagg agcggcggca aaaaaacaag gacggtcaat
      241 gaccgtcctt attttgtata atcgctccaa ccgtctattg cactgtcttg aattgctctg
      301 aaaagtcttt ttctaagtcc gggatacatt tccttttcaa gacgtttaat aagatctttc
      361 atatattgac gttgcgtata tccgtcagca ggacaaggat tgtgaacaac gggaatatca
      421 taatttttaa ccacacccct aatatcacgt tcgcgaacgt aaatcatagg tctgatttga
      481 tacatatcag tgcggtcgag atatgtaaca ggcgaaaaac aatgtattct gccctcgtgc
      541 ataaggctaa ggaagaatgt ttcaagcaca tcatcattat gatgaccgag tgcaacttta
      601 ttgcaaccgt tttcgattgc catatcattt aacgcaccgc ggcgcatttt tgcacacaat
      661 gagcaaggat tggattcttt tcgtatatca aaaataatct ctttaatatt tgttttctta
      721 accttatatt caacgcccaa atcatcacac attttttgta cttttgaata atcggcatca
      781 tatcccatat ccaaagtcaa tcccataacg gtaaattttt tcggatagta tcgcgaaagt
      841 tccgcaagca cattaagcag tacaagactg tccttaccgc cggaaacacc gacggcaatt
      901 ttatcgccgt cctcaatcat attatactgt tcaatagcac gccttgtaag acttactacc
      961 tttttcatat tattcagcct ttcctgttat tttaccgcct tgatagcttc ggttaagtca
     1021 accttatccg tataaagtgc ctttccaacg ataacaccct caacacctgt tgtaacaagt
     1081 gacttgatgt ggtcaagact tccgacaccg cccgaggcga taatatcggc atttaccgca
     1141 cttgccattt cagccattgc gtttacgtta ggaccggcaa gtgtaccgtc ggttgcaata
     1201 tccgtataaa cgattgtctg tacgccgata tttaccattt tctttgcaaa ttcaacagcg
     1261 gtaaaatcgc ttgttttttc ccaaccctcg atagcaacca taccgtcttt tgcgtcaata
     1321 ccgataacga tttttttgcc gtatttatca acggcacgtt taacaaattc actgtctgaa
     1381 acggcttttg tacctatgat tactctgtca ataccgcagg caagttttgc ctcaatatcc
     1441 tccatagtac gaataccgcc gcccgtttgt acgggaacat tgacgctttc gcatatttct
     1501 ttgatttttg cggcatttac gccatgacct tttaaagcac cgtcaaggtc gacaacgtgg
     1561 atatattcac cgccaagact ttcccatttt ttagccattt cggcagggga gtcaccgtat
     1621 acggtaacgt ccgaaaattg tcccttgaac aatctcacgc atttaccgtc ttttatatca
     1681 atcgcagggt aaattctcat tttgtaattc tctcctcaaa ctgttttaca atattcataa
     1741 tacgttctgt ttccattttc atactaacct tgtttacaac aaaacgtgca cttagagggc
     1801 atacgtcctc aagaacgtca agaccgtttt cgtaaagtgt tttacctgtt tcgacaatat
     1861 cgacaatgac gtccgaaagt ccgacaagcg gagcaagctc aacagagccg tttagcttta
     1921 taatttcaat agtctgacct ttgtcgtctt gaaaatattt cattgcaatg tgcggatact
     1981 ttgacgcaac acgaagattt gtcatagtgc caagcttacc ttttaattct ttcggaccgc
     2041 atacagccat tttacatttt ccaaaaccca aatcaaccat ttcatatagg ttacggcttt
     2101 cttccaaaag tgtgtcctta ccgacgatac cgatgtcagc cgcaccgtat tcaacgtaag
     2161 taggaacgtc agacgctttt acaaggataa atttcatctt gtttttttcg tcagtgaaaa
     2221 taagttttct tgttttttct ttcatttcgg aacaatccat tccgattgaa ataaataatt
     2281 ccattgcaag ttcggcaagt ctgccctttg caagagcaac tgttaaatat ctcattataa
     2341 tctcttcctc cctaaaatca gtgttcgtga ccgcagtcgc agtggtcgtg gtgatgttca
     2401 tgctcgtggt catggtcaca accgcagccg caatggtcgt gttcgtcaaa catcatagat
     2461 tcgtcatagt agcctaaaaa ttcgtttgcg gttgtttcta taatttcgtg ttttgcaaaa
     2521 tcgttaatca taagctgacc gttagggaaa actctaagca ttgcaccgat atttttacct
     2581 tttgaatatt cctctgctgc tttataatcg ccgtcaccga tatacatttc aacaaggcag
     2641 ccgtttacac gaagattgta tgcaatgtca taggcaatgc cttcggcgtt cttttcggca
     2701 aaaacaagtg atgcggaaac atcatgtacc tccttgtcgc ttagtacaga aataagtctg
     2761 tttataccga ttgccgcacc gacagcacct ttattgtcac caaattgtcc cataaggttg
     2821 tcataacgtc ctccggcaca aataggaaat ccgacaccgt gtgtgtagca cttaaagatt
     2881 gagcctgtgt agtagtcgat actttcaagc ataccaaggt caatagaaac gtacttttca
     2941 aaaccgtaca gacacaaaag ctcatatata cgctttaagt tgtcaagtgc gtttttggat
     3001 tcttcgttaa ggccgtcaac ataagccttt ttgaaaacct cttcaccgcc gaaaaggtaa
     3061 ggcaaatcaa tcataagatt tttgatattg tcatcaatat caagtttgtc tgtaattgtc
     3121 ttaataccga cagagtcttt tgaatcaata cgttcacgaa gtttttcgat agattgttcg
     3181 tcaagtcctg cttgctttac aagtccgttg aagaatgcaa tctgaccgat ttccattgaa
     3241 aattcttcaa tacctaccgc aagaacagcc tccatagctg ctgaaataac ttcagcgtct
     3301 gcggcaggtg agtatgagcc gataagctcg ataccgcttt gtgtaaattc tctttgacgt
     3361 gcaccttgtg tttgctcaac tctgtatacg ttgcctgtgt ataggtagcg ctgaggcttg
     3421 tctgaatttg caacctttgt tgccgccata cgggcgattg atgttgtaaa gtccggtctt
     3481 agtgcaagaa tacgtccttg ctcgtcaaaa aacttgaaca tatgctcttg tgaaatctga
     3541 ccgctaagac ctaaatagca gtcgtaatat tcaaatgtag gtgtttcaac ttctttgtaa
     3601 ccgattgaag aaaaaacaga ccatattgtt gattcgattt ctttttttac tgcacattcg
     3661 tccggcagta tatcatttac accgttcggt gtatgcaatt tccaattaga cataaaacta
     3721 atcctttcaa tatatttttg aattttaact ttattgtgct aaagcgataa agttattata
     3781 caacttaatt tgtaaattgt caagtaaaca atataatttt atcactaaaa cggtgtattt
     3841 tcaaggataa ggaatgtagt attaagtcga aatttgtaat tgagaaaaaa ttaacaaatt
     3901 gtcaacaaaa tttattattt aaactgttga atgttcacat aaaatgtgtt aaaatcaaat
     3961 tagtaaatat attgcagaaa ggaaatatga aatggcgata cacgttatta atgaagcaaa
     4021 aagatgcttg aactgtaaga aacctatgtg cagagaggga tgtccgatac atacaccgat
     4081 accggatatg atacatacgt ttttagaggg ggatataaat caagccggcg agatggtgtt
     4141 tgagaataat ccgctttcaa tcgtctgttc gcttatatgt aatcatgaaa atcaatgtga
     4201 gggacattgc gttttgaaca gaaaaggttc accggttcat ataagcagta ttgaaaatta
     4261 tatttccgaa aactattttg ataaacttgg tttttcgggg attgaaaaaa acggcttgaa
     4321 agcaggtata atcggctcag gtcctgccgg tataaccatt gcggtaatac ttgcacagcg
     4381 tggatatgat attacgatat ttgaatcaag agaaaaaata ggcggtgtgt tgagatatgg
     4441 tattcccgaa ttcagattgc cgaaatcaat tttggaaaga tataaggaaa aattgattga
     4501 gcttggtatt aaaatcagac cgaatacggc aataggtacg actataagcg ttgacgatat
     4561 gttccgtgac ggttacagtg caatatttat cggtacaggt gtatggaaac cgaacacact
     4621 tcatataaaa ggcgaaacgc tcggaaatgt gcattatgcg attaattatc ttaccaatcc
     4681 cgatgtttat agacgatact gttaatatta tcggtgcggg taactcggca atggacgttg
     4741 caagaactgc tttaagacac ggtgctaaaa aggttacggt attttcaaga agtgaccatt
     4801 tggcagcaag tcagcgtgaa gtggattatg caaagattga cggtgttgaa ttcgtggtac
     4861 acgttgagcc tgtggaaatt gtagatgacg gagaggcgtc ttatttaatt gtcatttctc
     4921 aaattatatt ggagagtcag ccatattatt tga
//
LOCUS       DAWACS010000057         4884 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1910_length_4884_cov_2.517291, whole genome shotgun sequence.
ACCESSION   DAWACS010000057 DAWACS010000000
VERSION     DAWACS010000057.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4884)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1910_length_4884_cov_2.517291"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4092..4101
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1552 a          862 c         1078 g         1382 t
ORIGIN      
        1 agaataatcg ccgcacttga aatgcttgca tcaaagcgtg attcaagacc ggctaaaaag
       61 catggaaatc ttcctgttta attgtgaaag gagattttta gtatgtattc agaagcatta
      121 ttgacaggtc agaacctgtc attaagtgaa gcgttggcaa tgggaggaca aacaacagtt
      181 ataggtttgg caatagtttt cgccgtactt atcatactta tgatagtgct ttcgcttttc
      241 aaggttatat tctataaaaa gcctaaaaca caggcagctc ctgctgcagc accggctcaa
      301 gccgcacctg tagaggaaga aacggcagat gatacagacg aagaagaact tatcgcagta
      361 ttgacagccg cagttgccgc aagtctaaat acttcaacgt ataacctaag aattaagtca
      421 tacagaagaa cagacaataa aatgcctgct tggaataagg cgggtgtaac cgaaacaatt
      481 aataatagat tctaaaggag gcaaaaccta aatgagaaag tttaatattt cagtaaatgg
      541 aaaatcatac gaagttgacg tagaagaagt aggcggagta tcaactcctg caccggctcc
      601 tgttcaaaca gccgctcctg caccaaaagc tgcaccggct ccaaaggctg caccgaaagc
      661 tgctcctgtt gcaggtgcta agcaagtaac tgctccaatg cctggtacaa tcgtatctgt
      721 aaaggtaaac gttggtgata aagttgaggc cggaacactt gttgccgtac ttgaggcaat
      781 gaagatggaa aacgaaattt tcgccggtgt agacggaaca gttgcaggta tcagcgtatc
      841 agcaggtgac agtgtaaaca gcggtgacgt aatagtttca gtaaactaat acagtcacga
      901 ctaaattata gaaagtggtg acaataaagt gttagaaagt tttgcaaact ttttgcagaa
      961 tacaggtctt tacgcactta tttcaagcgg tggcattgac gccgttaaga caattataat
     1021 gattgcgata gcttgcgtac ttctgtatct tgcaatagta aaaaaattcg agccgttatt
     1081 gctacttccg attgcgttcg gtatgctttt gtcaaactta ccgatgatta aggattcaag
     1141 tgctattatg atgcacacgg aattttttac gaatccggag tatatgacgg acggaaaata
     1201 tattaatgtc ggatatatct gtcagcatgg cggtctgctt gacctattgt atctcggagt
     1261 taagcttggt atttatcctc cgcttatttt cgtcggtatc ggttgtatga cagacttcgg
     1321 tccgctaatt gccaatccaa agagtattct tcttggtgcg gctgcacagt tcggtgtatt
     1381 ctttacattc gtaggtgctc ttgttctaag cgctcttgta ccgtcaatca atttcggtat
     1441 caaagaagct gcgtcaatag gtattatcgg cggtgccgac ggccctacgg ctatttacgt
     1501 tacaaaatca cttgcaccgg cgctactgcc ggcaatcgcc gttgcggcat attcatatat
     1561 ggcattgatt cctgtaattc aaccgcctat tatgaaactt atgactcgta aaaaagacag
     1621 aatgatcgtt atggaacaat taagacctgt ttcaaaaaca gaaaaaatct tgttcccaat
     1681 catcgttaca attatcgttg cacttctaat ccctgacgca gtgtcacttg taggttgtct
     1741 aatgcttggt aaccttatga aagaatcagg cgttgttgaa agacttacaa agatggcaca
     1801 gaacgaactt atgaatatca taactttgtt ccttggtgtt acagtaggtg ctactgcaac
     1861 agcgtcaagc ttcctatcag cacagacaat cggtattatc gtacttggac ttatcgcatt
     1921 ctgtttctca acagtaggcg gcttgttctt gggtgatatt atgtgctggg taacaaaagg
     1981 taaggttaat ccgcttatcg gaagtgccgg cgtttcagcc gttcctatgg ctgcccgtgt
     2041 atctcaaaca gtaggacaga aggaaaatcc ttcaaacttc cttttgatgc acgctatggg
     2101 tcctaacgtt gcaggagtta tcggttctgc cgttgcggca ggtgtattcc ttgcaatgtt
     2161 cggaaatctt taattaaaca gataataaag gagtgaaata taaatggcaa agggaaagaa
     2221 agctgtagaa attacagaaa cagtcctaag agatgctcac cagtcactta ttgctacaag
     2281 aatgacaaca gagcagattc ttcctattgt agaaaagctt gataacatcg gttatcattc
     2341 gcttgaggca tggggaggag ctacattcga tgcctgccta agattcctta acgaggatcc
     2401 ttgggaaaga ttgagaaaga ttaaggatag agctaaaaag acccctatcc aaatgctttt
     2461 cagaggacaa aacatacttg gttacagaca ctatgctgat gatatagttg aatatttcgt
     2521 tcaaaagtca atcgcaaacg gtgttgatat actaagaatt tttgacgctc ttaacgacgt
     2581 cagaaacctt gaatgtgcta tcaaggcttg taagaaagaa aaaggtcatg tacaggctac
     2641 agtttgttac acaacaagtg aattccatac aaacgaaaag ttcgttaaaa tggctaagca
     2701 gcttgaagaa atgggtgcag actcaatctg tatcaaggat atggcaggtc ttttaagacc
     2761 atatgtaaca tatgaccttg taaagatgat gaaagaagaa gttaagattc ctattcaact
     2821 tcatactcac tacacaagcg gtgaggcttc aatggtttac ctaaaggcta ttgaggcagg
     2881 tgtagacgta atcgactgtg ctatgtcacc gttggctatg ggtacatcac aaccgcctac
     2941 agagcctatg gttgcggctc ttcaaggtac agagtacgat acaggttacg atttggataa
     3001 gcttatcgaa atctgcgact acttcaagcc gctaagagaa gaatacatta agagcggtct
     3061 tatgaacact aaggttatgg gtgttgacgt taatactctt aagtatcaag ttccgggcgg
     3121 tatgctttca aaccttgtat cacagcttaa gcaacagggc agagaagacg catatgatga
     3181 agtgcttaaa gaagtacctc gtgttcgtgc agacttcggt tatcctccgc ttgttacacc
     3241 gtcatcacag attgtcggta cacaggcagt acttaacgtg cttatgggtg aaagatacaa
     3301 aatggtttct aaggaatcaa agggtcttgt aagaggcgaa tatggtaagt gccctgctga
     3361 aatctctgac gaaatcgtta agaagattat cggcgacgaa aagagaatta cttgtcgtcc
     3421 ggctgaccta ttaaagcctg agcttgaaca gatgaagaaa gaatgtgcag aatggattga
     3481 acagcctgaa gacgtattgt catatgcatt gttcggtcaa gttgccgtta aattctttga
     3541 atacagacgt gctcagaaat ataaaattga tgcagattta gtggatatgg aaaataagac
     3601 atatccggtt taattgcgtg tttaagtacc ggacgaaata tttcgtccgg tattttttat
     3661 aaaaattttt aaatacagga ggatattatg atttttggat ttataggcag cggaaatatg
     3721 ggcggtgcta taatcggcgg aattattaat tctgatatag caagtcctga aaacgtaatt
     3781 gtttcggata aaaatgagca ggcattaaag aatattaatg aaaaatacgg tgttaaagtg
     3841 acatcggaca ataaagaaac ggcagataaa gctgatgtat tgtttttgtg tgtaaaaccg
     3901 cagtttttat atggtgtgat tgatgaaatc aaaaacagtg tttcagataa tacagtaatc
     3961 gtttcaatag tcgcaggaca ggcgatagaa acgattgaaa aggcttttga acataatatt
     4021 aaacttgtgc gtgtaatgcc taatacaccg gctttagtcg gcgagggaat ggcggcattg
     4081 acaccgaaca gnnnnnnnnn ncaaaagaag aaacagatgt agttgtaaac gtgtttaaca
     4141 gctttggtaa aggtgaagta gtatcggaaa atcttatgga cgccgtaacg gctgtcagcg
     4201 gttcgtcacc tgcttatgtg tttatgatga ttgaggcaat ggcagacgca gctgttatag
     4261 gcggtatgcc gagaagtcag gcatatacat tcgccgcaca agcagtactt ggaagtgcaa
     4321 agatggtact tgaaacaggc aaacacccgg gtgaattgaa agatatggtt tgctcacctg
     4381 ccggaactac aatagacgca gttgcaatac ttgaaaaaga cggtttcaga aacagtatca
     4441 ttgacgcaat ggtaggctgt atgaataagt caaaagaact tggaaaaaaa taagataaaa
     4501 caataacgcc accgattttt attatcggtg gcgttattgt atatgaacgt aacattgtat
     4561 tcaaacaatg ttaaatatat tatacactat taattgtaaa aattcaatag aaaaataaaa
     4621 tctttttaaa aatatctctg atttttaaac attatcgaag tatgatatat attttacggg
     4681 tgcttgttta ttaaataaat actcatataa gtcgataaaa tagtcgtctg tcatacttgc
     4741 gatataatcc gtcacaatat cattaaaatc actgtttttt gtatattcgt atcccgaaaa
     4801 tctgtttgag tagtcgatta attcaatatg atgcttgaat atcggggatg attttatgtc
     4861 attttttaaa tcataaagca actt
//
LOCUS       DAWACS010000058         4883 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1911_length_4883_cov_2.071044, whole genome shotgun sequence.
ACCESSION   DAWACS010000058 DAWACS010000000
VERSION     DAWACS010000058.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1911_length_4883_cov_2.071044"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1223 a         1189 c          823 g         1648 t
ORIGIN      
        1 cctatcttct tatttctgac tttaaccgta actccgtcac gaagtacaat tatatcaaag
       61 ctgtcaggat atttatagct ttcaccctct gtcggaattt tgtcaccgac agtataagtc
      121 ttgtcaacag gttgatattt aagtactgac aagctctgtg gatcgtaata gaattcttca
      181 aaaatatcgc ctcttgcctc aaggttttca aacttaaacg atgctctatc cgcgaaagaa
      241 ccgccctttt ctttaagttc gtcaacaaca ccgcaagccg aagtcatatt tgtcacacgg
      301 tcgataagaa ttaattcacc gagtgttctg tgttcgtcaa acaagtctgc aacaacagcc
      361 tctgtaagca aaatttcgca taccgcaata ccgtttttgc ctattgtttc cgcattgata
      421 tgctcaccag tattaacgtc aatcgcataa tcaatacctg cgactatacc tgcgattgtc
      481 tttgtaccaa tctttacaag atagtctttt ccgactgtca aaggctcatc gtccatccac
      541 aaaagtgacg ctgtaagacg cttataaggt gcaaggtttg taccctttac aagcacacaa
      601 cctcttgaaa cgtcaacttc tttgtcaaga gtaattgtga caggctgacc tttaaatgca
      661 gtttctgact tcttgtcacc tacataaatt tcttttacat gagcactttc attgctcggc
      721 aatgttgtaa tctcgtcacc gacatttatg ctgcccgatt caatctgacc ttgaaaacct
      781 ctgaatgtgt ggttcggtcg gcagactctc tgtacaggga gataaaatcc ctgttccgca
      841 ttttcttcgc ttatatctac attttcaaga tattccaaaa gcggttcgcc gtcataccaa
      901 gtgatatttt ccgatttaac cgttacgttg tcaccctctg ttgcagacaa cggaattatc
      961 ttaacgtttt caagtgacaa ttcttttttc aattcttcaa tctgtcctac gattttatca
     1021 taagcatttt tatcatactt tacaaggtcc atcttattta cggcaaacac aaagtaacgt
     1081 atacccataa gcttacaaat tctcgcatga cgtcttgttt gaacaagcac gccttgtgac
     1141 gcgtcgataa gaattacagc caagtccgca aatgacgcac ctactgccat gtttcttgta
     1201 tattcttcgt gtccgggtgt atcggcaacg ataaagcttc tgttatcagt tgtgaaatag
     1261 cggtatgcaa cgtcaatcgt tataccttgt tctctttcgg ccataagtcc gtcaagtaaa
     1321 agtgagtagt caatcgctcc gcctctgctg ccgactttac tgtcaagctc cagcgctttc
     1381 tcctgatcgg cataaagaag ttttgagtcg taaaggatat gtcctataag tgttgactta
     1441 ccgtcgtcaa cgcttccgca agttataaat ttaagtaatc cgttcatatt agaaataacc
     1501 ctcccttttt ctgcgttcca tgcttcctgc cgcctcattg tctataactc ttgaagttct
     1561 ttcggaagat accgcactta gtgtttcttc aataatttcg tcaagcgtat cggcagtcga
     1621 ttctataccg cctgtaagcg gataacagcc aagtgttctg aatcttactg atttgtattc
     1681 aggcacttct ccctctttta gaggtaatct gtcgtcatca accattataa gattaccgtc
     1741 acgttcaaca acaggtcttt ttgccgcaaa gtaaagcggt acaatatcaa tcttttcacg
     1801 cttgatatac tgccaaatat ctttttctgt ccagtttgaa attgggaata ctctcatact
     1861 ttcgcccttg ttaatcttag tgttgtaaag tttccacatt tcaggacgtt gatttttagg
     1921 gtcccacgcc tgtgcctcat ttctgaatga gaaaattctt tcctttgcac gcgatttttc
     1981 ttcgtcacgt ctgccgccgc cgaatgccgc tgtaaagccg tactttgtaa gcgcctgctt
     2041 taatgcctgt gttttcatta tatcagtata agccgcaccg tggtcaaacg gattgatacc
     2101 ttgctttata ccttcctcat ttgaatacac aatcatttca ataccgagtt tctttgcggt
     2161 atcgtcacgg aatttaatca tatccttaaa cttccaagtt gtgtctatgt gcataaacgg
     2221 gaacggcggt ttttcaggat aaaacgcctt cattgcaagg tgcagcatta ctgaactgtc
     2281 cttaccgatt gagtatagca ttacaggctt ttcacactct gccgctacct ctcttataat
     2341 atatattgct tctgcttcaa gctcatcaag atgtgataat tcactcattt agtgtccctc
     2401 cttttagtgt cccttaatac ttatttgatt cttttctgtc tatgtctatt tcggaaaccg
     2461 tgccgtcata tttttcaatt acccatttca aaatatcgcc gttttcctct gtatgtacct
     2521 tacttcccat accgcctata tccgcaccga gataaaacga tattgcgtgc ataggacatt
     2581 ccttgataca ggaagtacag ccccagcaat ctttcgggta ctttataaat gctttattat
     2641 ctccgtcctt ttttataaga cttcccggac ataccataca gcattttccg cagcctatac
     2701 atttgctttt atcaatcatt atgctcatat gttctgcccc cttccacaag gtcacgaagt
     2761 ataatttcta tttcaccgtc tttaagacgt gaattgacgt atttgttaaa gttttcgtta
     2821 tccttattcg gataatcaag attttccgca aaactgtgcc agcgtgtttc ctttctcgct
     2881 ttgagatgtg ctataacact tcttgcaacg acaagtcttt cacgaagttc gtatatatac
     2941 ataagctcct gaaaatcttc tgcgtgaagt ttttctgaaa gctgatagat ttgacggatt
     3001 ttcgtatcgg ctatgtcaag ctgtttttca ttaaagcgat aatttgtctt gataccgcct
     3061 gcatacgaat ccataacggt ctgcattgcc tcttcaagct gttctgtcgt gaatgttccg
     3121 tcaatgtttc cgtaaaggaa atctgtcact tccgacaggt gacgttcaat gtcactttcg
     3181 tcaatcgcac ttatttcgcc aagttcagaa attgcggtaa gtgccgcgat ttccccctct
     3241 gccaaagcac ctgtaacgta tttctgcggg caaccgcctg caacgtcacc tgcggcatac
     3301 aatcctttta cggtagtcct tctctttgta tccacccaat aaccgcttgc ggtgtgaccg
     3361 cctacgatat aaggctctgt accctctatc tcaacatttt gctctgacgg atttttaccg
     3421 ctttcaatcc atttaagagt ctgtgacggt gccatattca gataagcctt tttcaaatcc
     3481 tcgcttgcct cttgggatat accctctgta cgaagatagc aaggacctct gccctcaata
     3541 ttttcattca ccgttccgta cactctctcg gaggttgtaa gaccgtattt tgtttcgtaa
     3601 acctcaccga gtgaatttat ttgctttgca cccgcacctt gtgccaaagt acctgtcggg
     3661 gcgattgtat ctttacagcg aagtgctata aatcgcattt caaatgtcgt catttccgca
     3721 ccggctttta tacccattgc atatcccgca cccgtattaa acggcggata ccacatttta
     3781 tgacgtgaaa atccgggatt attcggcttg taaaggccgg ccgcaccgcc tgttgcgatt
     3841 ataaccgctt tcgcttcgat tatgtaaaac gtatcgtttt caatacctat accgtatgca
     3901 ccctttaccc tgttaccctc aaccgcataa tcaataatat ttacacgatt aagtaccgat
     3961 acattttctt tttgcaacgc cgcctctgcc aaaataggct ttatattttc accgttgatt
     4021 ttaaggtttc tgttacctct tgtcacatac tttccgtttt cgtccttcaa aataacaagt
     4081 ccgagttttt caagtctgtc tacgacttca ttcagctttt cggacattgt aagaagtaaa
     4141 tcttctctga caattccgtc agcgtccttt ttagcataat caacgtaatc ctgcggaaca
     4201 cgtccctctg ttatgtatgc gttaagtgcg ttgacacctg cggcaagaca accgcttctc
     4261 tttatatgtg ccttttcgca tataagcacc ttagcatcgg aatgttctgc gatagtaacc
     4321 gccgcatagc aaccggcagt accgccgcct ataattaaaa tatctgtttt taatctttcg
     4381 gttttcaata tatttgtcat ataaacacct cttttatcag attaccaatg aatcctccga
     4441 aattgattta ttgtcaagca agtatgcctt atcgcctacc gtaacaaata ttctgtacaa
     4501 gaatacgtca acttcttcac cgtcttcaat cgcctgttcg tcaagaccgc gtctggcttt
     4561 aagttccgta ccgtccacgt ctacaactac ttctatactg tcgcctctga acaatacatt
     4621 tttcgtaact ccgtgagatg cggagctttt gtatttctgt acttcgtttt tctttgtcac
     4681 tttaacaaat tcaggtcgta cgattgcttt ttcgacattt tcaatatgct caaaattatg
     4741 aaactttgaa taatcatcaa cgaatgttgc ttctccgaaa aacgatgccg taaatgctgt
     4801 tttcggattg tgataaattt caataggtgt tcccgtctgt tcaattctac ccttatttgt
     4861 tattataatt tcgtctgcaa ctt
//
LOCUS       DAWACS010000059         4866 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1918_length_4866_cov_2.952193, whole genome shotgun sequence.
ACCESSION   DAWACS010000059 DAWACS010000000
VERSION     DAWACS010000059.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1918_length_4866_cov_2.952193"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3029..3038
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1621 a          756 c         1203 g         1276 t
ORIGIN      
        1 ggtcaaagaa ttgacttgag caatatcgac cataccaata cggaagaaga gtatgttgta
       61 aagacagagg aaaagaaacg ttatgttgat acccgtcaat caactgttga acttgataca
      121 atagaatccc gtgaacgtat tgaagatatg gttcctgata atatcaagat ggataaaaag
      181 ggcggtaaag ctaagaataa aaagggcggc aaaaaccgtc gtgaaaagga aaagaaccaa
      241 cagcctaaaa aggaaataaa ggctaaggtt ataacagagg ttgaaatccc tgaaacaatt
      301 acggttggtg aatttgcgtc taagatgggt aagacatcgg cagaagttgt taaaaaactt
      361 atgatgcttg gagttatggc atcacaaaac caagctatcg actttgaaac agcacagctt
      421 atcggtgacg atttcggtat tgaagttaaa caggaagttg ttcttacaaa agaagatatg
      481 cttatgattg aaaccgagga agaagataag ccggaagacc ttgttccgcg tcctcctgta
      541 gttgttgtaa tgggtcacgt tgaccacggt aagacatcac ttcttgacgc aatccgtgac
      601 acaagcgtaa cagatacaga ggctggcggt attacacagc atatcggtgc ttacagcgtt
      661 aaactaaacg acagacttat aacatttctt gatacaccgg gtcacgaagc atttacaaca
      721 atgcgtgcga gaggtgcaca ggtaactgac gttgcaatac ttgttgttgc ggctgatgac
      781 ggtattatgc cacagacaat cgaggctatt aaccatgcaa aagcggcagg tgttacaatc
      841 gttgttgcaa ttaataagat tgataaagac ggtgctaacc ctgaaagagt taagcaaatg
      901 cttgttgagt acgatcttgt acctgaagaa tggggcggag atactgtctg cgttgaaatt
      961 tcggctaaaa agagaattaa cattgacgga cttttggaaa tggtacttct tgttgcagat
     1021 atgcaggagc taaaggctaa ccctaacaga gatgcacaag gtactgtaat cgaggctgaa
     1081 atcgataagg gcagaggtcc agttgcgact gttcttgtac agaacggtac acttaaagtc
     1141 ggtgattttg taattgccgg aacagctgtc ggcagagttc gtgcgatggt aaacgataag
     1201 ggcagacgtg ttaagactgc accaccgtca acaccggttg aaattcttgg tttgtcggag
     1261 gcaccgtcgg gcggcgacac atttatggtc gttgaaaacg aaaaacttgc acgtgacgtt
     1321 gctgaacaga gaaaacagga acttcaagaa aataaattta atcaagttgt taaagtatcg
     1381 cttgataact tgttcagcca gattgacgag ggtaacatga aagagcttga cgttatcatc
     1441 aaggctgacg tacaaggtag cgttgaggct gttaaacagt cacttgaaaa attgtctaac
     1501 gatgaagtca gagtaagagc aattcacggc ggtgtaggtg cgattaacga atctgacgta
     1561 atgcttgcaa atgcgtcaaa tgctatcatt gtcggcttta acgtaagacc tgacgcaggt
     1621 gcgcttgcgt ctgctgaaca gaatgaagtt gatataagac tttacagagt tatttatcag
     1681 gcaatcgaag aaattgaggc tgctatgaag ggtatgcttg accctgaatt taaggaagtc
     1741 gttatcggtt atgcggaagt acgtcagaca tttaaggtgt caaacgtggg tactatcgca
     1801 ggttgttatg taacacaagg caaaattcag cgtaatgctg aaattcgtat cgttcgtaac
     1861 ggtatcattg ttcacgaggg tactataaat tctctaaaga gatttaaaga tgacgcaaaa
     1921 gaagttgcgg aaaactttga atgtggtatc ggtgttgaaa acttcaacga tattaaagag
     1981 ggcgatacta tcgaatgttt tgttatggaa gaaatagaaa gatagttatt ggctgaaatt
     2041 cagccattca tcggagagga ataaatatgg caagaattga taaaatcaat gaagaagtta
     2101 agcgtgaact tgcaaatata attcgtgaat taaaagatac gcgtattcca cttatgacga
     2161 gcgttgttac tgtaaatgtg acaaacgact tgagatatgc aaaggcatat atttccgtta
     2221 tgggtgatga agaaacaaag aaaaaagcta tgcagggctt aaagagtgcc gccggcttta
     2281 taagacgtga attgggtaaa cgaattgatt taagatatac gccggaattt gtgtttgaac
     2341 ttgatgattc aatcgagcat ggtgcaaata tcgaaagact tcttaataat ataaacaaga
     2401 aataaagggg attagttatg caggctgtta ttgataagat aatggaggca aaatccgtag
     2461 cagtgcttac tcatttgaat gaggatccgg atacaatcgg ctcatgcttt gcatttgcaa
     2521 aggttatgag aaagttgggt aaagaggcga ccgtatatgt aaacggaaga attgagtcaa
     2581 gattggcatt tataggtgat gattatgttt tgtatcaaga gggtatgaaa cataatcacg
     2641 acctttgcgc gtgcattgac tgcggtgatt tgggacgtat agcagagaga aagtccctct
     2701 ttgaagaaat aaataattca ataaatattg accatcactt aacaaacact aattttgccg
     2761 atgcaaatta tgttgacggt aaagcggctg cggcaggtga aattttgtat gctttatttg
     2821 aaaaaatggg cattgaattg gataatgata ttgccaagga tttgtatacg gcaatatgct
     2881 ccgatacggg ttgctttaag tacagtaacg ttacgtctaa aacaatgcgt acggcggcaa
     2941 accttttgga atatgatttt gaccatgcgg aagtggcaag actgcttttt gacagtgagt
     3001 cgcttgcggc ggcgaaatta aaagcagann nnnnnnnngt aaggaagata ttccgaattt
     3061 ggttgatatt ccgagaagaa ttgagggtac tgaaattgcg gtttgcataa aacgtacaaa
     3121 caacggtttc agacttaatt tgcgttcaaa cggtaaagcc gacgttgcgg aagttgcaat
     3181 gaagttcggc ggcggcggtc atattaaagc ggcaggtgcg actttggatt tcgattcaac
     3241 cgaaaaagcc gaaaaggctg ttatcgacgc ttgcaaaaga gctttggata aaataattta
     3301 acggagatta taatgaacgg tataattata atagataagc ctttgggacg tacttcgcat
     3361 gatatggttt atgttatgcg taagatgacg ggtataaaaa aggttggaca tacgggaacg
     3421 cttgaccctg aggcaacggg cgtgctgcct gtttgtatcg gctctgcaac aaaggttgcg
     3481 gatatgctta ctttgtcgga caagtgttac agagctgagc ttgttctggg tatgacaacc
     3541 gatacgcagg acgcagacgg tgaagtgctt acggaatgtg aagtgacttg cagtgaagat
     3601 gaaatcagac aagctataat gagctttgtc ggtgaaatcg agcagatacc gcctatgtat
     3661 tctgcgataa agcaaaacgg caagaaactt tacgaacttg caagaaaagg tatcgaagtt
     3721 gagaggaaaa gccgtaaggt gacgataaat tctattgata tacttgaaat taacggcgaa
     3781 cgtgtgacta ttgacgtttc gtgttcaaaa ggtacatata tcagaacact ttgcgaggat
     3841 ataggtatga aactgcacgt cggtgcgtat atgaacacat taagacgtac aaaaacagga
     3901 ccgtttacga tagaagaaag ccacacgcta aaggaaatcg aaatattgaa aaacaacggc
     3961 gagcttgaaa aaatcataat tcccgttgac agaatgtttg aggaatatcc taaaattaaa
     4021 cttaacccaa agcaagttaa aagcgttacg aacggtgtac agatgacgta cagcggtaaa
     4081 gagggacaat cttaccgcgt gtatgacgaa aaaaataatt ttttgtgtat atcaaagatt
     4141 acagacggca aattaaaact tgaaaaatct ttctggaatt aagggggcga tataaatgaa
     4201 gatattccgt gataaaacag attacgatgg tacggttgtt gcactcggaa attttgacgg
     4261 acttcatatc gctcatatga cgattatcag aaacggtatt aaatatgcaa aagaaaacgg
     4321 cttaaaaagc ggtgtgctgt tgtttgaaga aaacacaaat gacgttgtgg ggcataagat
     4381 tgagcttata acgccgaatg aggcaaagtt cgagcttttg gaacgtgaaa agcctgattt
     4441 tgtgtatatg gagaaatttg ataaggagtt tatgaaaaaa actcccgaag aatttgtaca
     4501 gtatcttgtt gaaaatcttc atataaaggc tgtttgtgtc ggatatgatt acagtttcgg
     4561 ctacaaagca caaggtgacg tcaaactttt aaaatggttt ggcgagaaat acggatttaa
     4621 agtgtttgtt acggacgtta taaagcttga cggaaaaatc gtttcgagta catacataag
     4681 gagcattata aaagccggtg atatggaaaa agcggaacgt tttttgggca gacgttattg
     4741 cattgaaggc aatgttgtga aaggtttgca gaacggcaga aaaatgggta ttccgactgc
     4801 gaatgttgat tatgacgtga atatggcact tcccgaagag ggtgtttacg caggtataac
     4861 gtatgt
//
LOCUS       DAWACS010000060         4846 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1927_length_4846_cov_2.315800, whole genome shotgun sequence.
ACCESSION   DAWACS010000060 DAWACS010000000
VERSION     DAWACS010000060.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1927_length_4846_cov_2.315800"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    502..511
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    930..939
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2033..2042
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1276 a         1003 c          947 g         1590 t
ORIGIN      
        1 aagtatttca aacgaagaaa gagttatttc atagccgtga ttagcacgtt tttcttcttt
       61 tacgtcacct gtgattttga tataagcacc ctcacaaagt gagtcaaaat cgcctgtaca
      121 tttatccttt gaataaacgg attgaagaat atatcttccc gtacgaagta ttacaaatga
      181 gaaaccgctc atttttctaa gcttatgcac gcaagcacaa aagcttattg aaccgccgac
      241 agctttttgc aaatcgtcaa gagtgatttc gcttataagc tgtgaaccat ctattttctg
      301 catattaatt ccacctttat tttaattttt cagtcaataa gtccttagca agtttagggt
      361 ttgcaccttt acccgccgtg cgtaccactt gtcccataag gaaaccgaaa atcttttgat
      421 taccgttttt gtagctttca acactgtctg cgttttcggc tataatttta tcaataattt
      481 cttcaagacc cgatgtgtca tnnnnnnnnn nccgccgtta ttgaacataa tcttcaaaat
      541 atccttggcg gcatttttgg aaacaacgcc gtctgttgac attttaacga gttctgccaa
      601 atcggcaggg gagaatgtta catctgaaat tgtcttttca gaatcgttaa gatttctgtt
      661 aagttcaaca agcattagat tagaaatttg tttgtagtcc ggatttattt ttacagactc
      721 atcgtaaaaa tccgaaaatt ccttgcttga tataataaga ttagcgtcat ctgtcggaag
      781 tccgtacttt tccgtataac gtacaaaacg gtcttgcggc atttccggca ttgactttct
      841 tatgtcctca atttcttcat ccgataggaa taccggagga atatccggct cagggaagta
      901 acgataatcc tggctgtcct ctttatcacn nnnnnnnnna ccgattgctt ttaatgagtt
      961 cagattttta agttcggcac gggtaccaaa ttcggttgag ccgacaggca taattgaaat
     1021 attaacgtca acacgcattg agccttgctc catttttgcg tcacacacgc ctgcatactt
     1081 taaacgcaaa gcaagttttt caacaaagtc ttgaacttct tcgattgaac ggaagtccgg
     1141 ctctgttacg atttcaataa gcggtacacc gcaacggtta tagtcagcca ttgaaatgcc
     1201 ctcataatcg tcatgcacaa gcttacccgc gtcctcctca atgtgaatgt ggttaattcg
     1261 gatgtcctta ccgcctgtga cggttatata accacctgta cagatcgggt ggtcaaattg
     1321 tgttatctga tatgctttag gcaagtcggg ataaaaataa ttctttctgt cgaatgcact
     1381 gtaattattt acggtacagt caagtgcaaa acctgccttt gcggcaagcc ttaccgcacc
     1441 ttgatttaaa aggggaagtg tacccggaag tcccgaacat cttggacata cacgagtgtt
     1501 ttcgctgccg ccgaattcat tctgacaagt acagaatact tttgaatctg tcaataattc
     1561 cgcgtgaatt tcaagtccta ttacgggttg gtatctcata gtacaatctc ctttctttca
     1621 aagtcttttt caaatctgtc gcatactgct attattgtct tttcgtcaaa tgcttttccg
     1681 ataaggctca taccaatagg caaacctttt gaatcgtaac cgcaggttgt agaaattgcg
     1741 ggaagtccgg caatgtttac cgttactgta taaatatcgg caagatacat tttaacggga
     1801 tcgttttcct gttcaccgat tttataagcc gtagtcggtg cggtcggtgt aagcataaca
     1861 tcgcacttgc tgaatatatc ggcatattcg tttcttattt gcgttcttat acgcgttgcg
     1921 tttttataat atgcgtcata gtaacccgaa catagtgcgt agttgccaag cataattctt
     1981 cttttaacct cttgaccgaa tccctcacgg cgtgaatttt taataaggtc gtnnnnnnnn
     2041 nnttctaaga ccgtatttga taccgtcaaa acgtgaaagg ttgcttgccg cctcggctga
     2101 tgaaattagg tagtaagccg aaaccgcata ttcaagactc ggaagtgaaa cgtcaactat
     2161 ttcacagcca agttttttgt aatattcaac ggcgtttaaa acagccgttt taacttcgtc
     2221 atttagaccg tcaccgaaaa attctttcgg tacaccgatt tttaatttgt ttacatcact
     2281 tccgataagt gaattatagt tgccctctga ttttttgctt gatgtggcgt cattttcatc
     2341 atagccgtaa atgccctcaa gcatatagcc tgtgtcttcg gcagactttg cgataacgcc
     2401 gatttggtca agtgaagatg cgaatgcgat aagtccccat cttgaaacag tgccgtatgt
     2461 cggtttaaga cctgtaacac cgcagaatga ggcaggctga cgaacagaac cgcctgtatc
     2521 cgaaccgagt gcggcggcac aaaggtcggc acttacgctt gctgcggcac cgccggaaga
     2581 accgcctgta acacgagtta agtcgtaagg gtttttaaca ccgccgaaat atgatgtttg
     2641 agaagaacca cccattgcaa attcgtccat atttgttttg ccgagcataa cgatgttgtc
     2701 tttctttaat ttttccatta ccgttgcgtt atacggagga atgaagtcat caagcatatg
     2761 tgatgcacag gttgtcttga caccgagtgt acatatgtta tctttaaccg aaataggcaa
     2821 tcccgtaaat gcaccgctgt tgccactgtc gataacttcc tgtgctttct ttgcgtccgc
     2881 aagtgcgttt tctttgcaca ctgttatata actgtttatt tctttatctg aattttctat
     2941 tctgtcaaga tattgctcgg taagttcaac cgcactgatc tccttactgt caagcatttc
     3001 tctcagtttt tttaaagcca cgttaatcaa atcctttctc ttgactgtta aactacttta
     3061 ggaactacga aactgttatt tttaacattc ttcgcattct taacaatttc ttcggtaggg
     3121 tacgaatttc gatgttcgtc cgcccttaaa tctttatagt caaccgcatc aagtgcatag
     3181 gcaggctttg atgtatcaaa ttcacatact ttatccatga tagctatgat atttgtcata
     3241 tcagccgtca tttgttctag ctcttcatct gtaaattcaa gctttgaaag ctctgcgagg
     3301 tgctttgtca aatctgagtt aaacatatca attctccgtt ctttaaatat tttgtttttg
     3361 tataatgttt ttattataca aaaaaagacg taatataaca gagttttcgc tctgttatac
     3421 aacgtctttg ctgtaagatg aatattataa taccataatt taaaaatgtt tgcaaggggt
     3481 aaaatgaaaa aatattaagt tttatattat tttcatttat gcaatttgca ttatggctcg
     3541 tgcggcatat atgtacttgt attaagcgta tcggttgaga taagctcacc gttgacatat
     3601 acatatcttg ttgaatgaac aacggtattt ttgccggaag ttgaagaagt gtttgaaatt
     3661 ttaacttctc tgtgaggttc ccaatcagta ccgataatgc ttacagtgat tgttgcaccg
     3721 cttgtgtaag ccgaaacttt aatcggatag tcggtgttgt ttttaaattt gaaatcaact
     3781 ccgccgtccg aaacggttgc gtcctgaccg agcggaatat aacctacggt catcatatgg
     3841 ttaagacgtt caacaatcga aaggtcggca tacaataccg ccgaataaag ggtactgctg
     3901 acttgacaag taccgccgcc gataccgtca acgctcttgc cgtcaacgta ttcttttgcg
     3961 gtagagaaac cgttttcgat tgtgcggtga ccgactgtgt cgttgaatga gaatacatca
     4021 cccggtgcaa ttactttgcc gtttataagg cttgccgctc ttgcaacatt ggcacatctg
     4081 tttgcggtcg aactgccgta tgatgttgaa taactgctca atgttgccga aaataatttt
     4141 tctcttaatg tgttttcgtc aacggtcgga tatgaaacgt agaacggcaa tgtgacaggt
     4201 tcacagcctt cgtatacatt tcctataatt gctgcagcct catctttatt tatgtaacgt
     4261 cccgtatcgc ctgcaactat gtttacatca tttccgttga cttcgtatct tgcgtctgtc
     4321 gggtctttgt atacaagtgc gtcaagtgat tcaacagtca tttcgtccgg tggggcagaa
     4381 gtgaatgtta ccgcgatgtt attaaaaatt tcattgctaa gagcggtaat aacttcttgc
     4441 tcggcggttg atgggtctaa attataacct gtcttaccgg aatatattgt aagtgtaccg
     4501 tcgtcgttaa attcaacgta atgctgtttt ctttcgccaa gtgcaatgtt accgaattcg
     4561 ttaagctttg cgtcaagctg ttcggtatcg acttgtacgg caggaacgat tatgtgtggc
     4621 ttgaacataa gttcaatagc ggcaaatgca ttttttaatg tgctgtccga tttgcagtaa
     4681 tcgaaagcct tttttgcggt atcttccgca attgctgtaa gtccgatgtc tgagccgttt
     4741 atttcgtatg tctgaccgtt tgaagtcaat gtgattgcag tattctgtaa aagatatgtt
     4801 gcgtttattg aatcaagtgc ctcgtcatat gtaagaccgc caacgt
//
LOCUS       DAWACS010000061         4825 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1937_length_4825_cov_1.962683, whole genome shotgun sequence.
ACCESSION   DAWACS010000061 DAWACS010000000
VERSION     DAWACS010000061.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1937_length_4825_cov_1.962683"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1414..1459
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3634..3643
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1712 a          727 c         1087 g         1243 t
ORIGIN      
        1 atcaaaaatc gtgtttgcaa gtgcagatat tccgcaggct gattgggttg ttgtaaataa
       61 aacagacaac tttgcagata agatggatga aatcgaaaac aagcttggtt atccttgctt
      121 tgttaagcct gcaagaacag gttcgtctgt cggagtggga aaggctcacg acagaaaaga
      181 attaaaagcc gcacttgaaa atgccgcaca gtttgacaga aaaatacttg tagaagaaaa
      241 tatcgaaggt catgaggttg aatgtgccgt acttggtaat gaggacgtaa aagccgcaac
      301 agtaggtgaa attatgccaa ctgttgaatt ttatgacttt gatgcaaagt acaacgacaa
      361 ctcaacagaa cttcaaattc ctgccgatct gcctaaggaa acaatcgagc aaatcagaga
      421 gtatgccgta agagcgttta aggcacttga cggcagcgga ctttcaagag ttgacttttt
      481 tgttaagcac agtgacggaa gtgttgtatt aaatgaaatc aacacacttc cgggatttac
      541 aaacataagt atgtatccga aactatgggg tgctgtcggt atagaataca gcgagctttt
      601 agacaaactt atcgagcttg cggaaaagag agtaaaataa tggataacaa atgtataggt
      661 gtttttgatt caggacttgg aggacttact gccgtaaagc agataataaa tgagttaccg
      721 aaagaggaca ttatatattt tggtgatacg ggcagagttc cttacggctc gcgttcaaaa
      781 gaaacgttgc ttaaatatac aagaggcgac atacgttttc ttaacacatt tgatgtgaaa
      841 attatagtta tcgcttgcgg aactgcaagt tctgcggcat tgcctgcgat taaggacgaa
      901 tttgacgtgc cgattatcgg cgtaattgac gcggctgttt atgcggcggt aagagcgaca
      961 aagaacaaga aaatcggcgt tataggtacg gcgggaacaa tcaaaagcgg tgaatacgaa
     1021 aagcagataa aggcatatga cagagagatg cagacttttg caaaggcttg ccctatgttt
     1081 gtaccgcttg ttgaaaacgg atattttgat acggaggtta cgcgaattat cgtggcggaa
     1141 tatcttgaag aaataagaaa tcaaggtgtt gatacactta ttttgggctg tacgcattat
     1201 cctttgattg aaaaggttat aagagaatat atgggtgatg acgttacact tataaattcg
     1261 ggtgcggagg ttgcaaagta tttaaaaaag aaacttacaa aagaaaacct tttgcatgac
     1321 ggcaagtgtg acgaaaatca atacagatat tatgtaagcg atgatagatc aagctatgag
     1381 gagctaggcg gaattttcct tgaaagagaa attnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnna acgaatatcg tataacggtc agcaacagac agacgattga
     1501 ttcggaaacc gacacaatag aagaaacggc ttacggaaat tacaccgaaa aaaacggtaa
     1561 gcagtatata acgtacaaaa cagaaaatga cggagataaa atttcgtcta tgataaaaat
     1621 agacggcaac gagatactta taaaacgtac gggaagtgta aattcgtcaa tgacgtataa
     1681 gattgataca aagaaagagt ttttgtatca tttgccttac ggcacagtgc cgatggagat
     1741 tgaaacgcaa agaatagtat caaaacttga cgagaacggc ggaactatcg agcttgttta
     1801 tacacttacg gtacagggcg aaaaatattt taacgatatg aaaataacag taagtaagag
     1861 gtagtgcata tgaagaagaa attgacagcg gctgttttgt ctgttgttat gttgcttatg
     1921 tcgggaagtg tttttgcggc tgaaacagaa acagcggaag aatcaaaata cagcggtgaa
     1981 tacggtgcat ttgaacaaat ggcaaaatat gttgccgaaa gatatataga cgattcactt
     2041 acggaagaag aaataatgaa acagggtctt tcaaagttgc ttgaaaataa ttacccattg
     2101 ttggtgcagt tgttgaagtc aatgcttgaa agccttgatg attacagtga attttacact
     2161 ccggaggagt ataaggcatt tcaggataaa ttaaatcaga atttttacgg tatcggtata
     2221 tcaatgaaaa tgtctgatga cggttatgtt gaaattgtgg gttttctcaa tgaggacagt
     2281 aaggctgaaa aagcaggact taaaatcggc gacaagattt gcaaggttga cggcgagaac
     2341 gttacgggct ggagcatatc cgaagtgcgt aacaaaataa tcggcgaaga aaatacaagc
     2401 gtcagagttt ccgtgctgcg tgacggggaa gaacttgaat ttataacaac gcgtgttgcg
     2461 gtacatgaaa attccgttaa ttcggctgaa ttgaagggta atataggtta tattcaaatt
     2521 acaaccttta attcgacaac taccgatgaa tttacagatg cacttgattg gatgcgtgaa
     2581 aagaatatta agaagataat tcttgatttg agaaataacg gcggcggtct tgtgtcatca
     2641 tcggttgaga ttgcacagca gattgtaaaa aaaggtaaga taatcgacgt taaattcaga
     2701 gatacaaagt ataacgttac atatgaatca aagcttgata agcctgaatt tgactttgcg
     2761 gtacttgtaa atgagcatac tgcttctgcg tctgaaattc ttgcaagtgc aattcaagat
     2821 tccaagggcg gtactcttat cggaacaaag actttcggta aggcggtaat tcaaaataca
     2881 tatccgcttt caaacggttc tgtttttaaa cttacaacag gtcagtatgt gacaagaaac
     2941 ggtaaggaaa taaaccatat cggacttaca cctgatgttg aagttgaaaa tactacagac
     3001 agaatagata catcaaagta tacgccgttt gattatacaa caaaacaatc atacggtaat
     3061 tcgtccgata atgttaaagc cgcaaaggaa agactttatc tgttggattt ctataacgga
     3121 aatacggaca gtgatgtatt tgacgatgaa ttgaaaactg cgataaagga cttccaaaaa
     3181 gcaaatgatt tgctttcata cggagtcctt gacataccga ctcaaaagaa aattgaaaaa
     3241 gttttttcaa agattgaagt gacaacagac aatcagtttg aaaaagcata cgaattgatg
     3301 ggcggaaatt tagaagagca aaaagctctg ttgtttgcgt taaagactaa aaatattccc
     3361 atagacattt tggaataatt aaaattactc tgaaaagaat atagttagag tattaagaaa
     3421 tgtccggagg aatagatatg aatagtgtaa taatgataac acagtttttc tttacacttg
     3481 ttataggaat gtattttttc acaaagctga ggaatgaaaa aagcgacagt gaaacacttg
     3541 caaacaattc aaaaaaagag gctgaacacc ttaattatat gcgacatata catttgtctg
     3601 aacctgtaac ggaaacggca agacctaaaa acgnnnnnnn nnnaaaacgg cagcggcaag
     3661 agtggcgttg gaatgtgcaa agaaaagtaa cggcacaccg ttttcaaaaa atgcgaagtt
     3721 tatcgaaaca gacgcaacga taatgcgata tgatgaaaga agcattgccg atccgcttat
     3781 cggctcggta catgatccga tatatcaagg tgcaggcgca tacggcgttg caggtgtacc
     3841 tcaggttaaa gagggggcgg tgtcaaaggc tcacggcgga gttttgttta tagacgaaat
     3901 cggcgaatta cagacttgcc agattaacaa gctgttaaag gtgcttgagg acaggagggt
     3961 tatgtttgaa agtgcatatt acagcgaaga aaacaaaaaa atcccaacgt atatacacga
     4021 tgtattcaaa aacggcatac ctgcagattt cagacttatc ggtgcaacaa ccagaaaacc
     4081 ggaagaaatt cccgaggctg ttcgttcaag atgtgtcgaa atttatttta accctttgac
     4141 gagggataat atagaaaaaa taatttacgg tgcggctgaa agaataaaaa tcaatataga
     4201 acaaaatgcg gttgagatga ttgcacgtta tgcgaaaaac ggcagagatg cggttaaaat
     4261 tcttcaaatg gctaaaaata ttatatttct tgacgacaca agaaacgtaa ctttggacga
     4321 tattgcgtgg attttaaaaa cgtgtcatta tacaaacgga tatataagca gtgatgaaaa
     4381 aataattgat atatcggtca taaaacctaa ataaaaaact gtaaaaaaca gttgaaaatt
     4441 atacgaaaaa attatataat agtaatatta atcttatagg agtgaaaacg gattggaaaa
     4501 agaactaaaa cgtgtgtcgg tagtgccaat gcgtggaatg gttttgttcc cgcatatgac
     4561 gcttaatttt gacgcggcac gagaaatgtc cataaaagca cttgaaagtg ctgctgaaaa
     4621 tgattcaata gtattccttg cggcgcaaaa gaattataca atagaacaac cgactgttga
     4681 cgatatatac agtataggta cgtttgcggt tataaaacag gttatgcgta tgcccggcgg
     4741 tataacaaga gttatagtaa agggattgga acgtggaatt attgaaaatt tcatttccga
     4801 aaagccgttt tttgaggcag aagta
//
LOCUS       DAWACS010000062         4819 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1939_length_4819_cov_2.726700, whole genome shotgun sequence.
ACCESSION   DAWACS010000062 DAWACS010000000
VERSION     DAWACS010000062.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1939_length_4819_cov_2.726700"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1373 a         1134 c          827 g         1485 t
ORIGIN      
        1 atacttgctc aatgcgaata ttttacagtc cgcaagataa ccgtagctga tactgcgtca
       61 atagaccttg atacaaccag tttccgttcc ctgatagtaa ccgagggcag cgcaaagctt
      121 gaaataaacc acgaaacgct ttcacttgaa aaaggcaaca gcattttcgt tcccgcgcag
      181 tcaggcagat ttaatattta cggaaactgc actctgatta tgtcgcaggt ataatatatc
      241 ttagtatact tatgcggcaa ctaactgatg aattaatatt ttacaaggag atttttaaga
      301 attcgtcttt atatcttgag ttgtccaaag aagaattaag gaaactcata agcgaaacgc
      361 tgaatactga cataaattcc gccaaactgc ttaccggagg attgttcaat accacttatt
      421 acatagatac aatgaaatat ggttcagtag tgcttcgtgc aggtcctgtt aatcggccct
      481 tgcttcttcc gtttgaacac agcctaatgc aagctgaaca gttggtatac aaattatgcc
      541 gagaagcgca agttcccgtt tccgaaatct tgtttatgga cacttcaaaa agaatcattg
      601 attgtgatta tatgattgtc agatatattc caagcgtacc aatgaatgaa gcagtattat
      661 gttctgataa taactcacaa atttgccgag atattggagc cgcaactgca aaaatgcact
      721 caattacaca ctctcatttt gggcgaattg tagatgttga aaatggaaaa gggttttcta
      781 aatggagtga ttgcttattg cacgaattaa atcaatggga acgcctgtca aaatcaacct
      841 ctctcttttc ggatgctgaa tatataaaaa tcaggcgaac ttttgaaaat tctattcctt
      901 tcttggataa aatccaaact cccagccttg tgcatactga tctttggaaa ggaaatgttc
      961 tgatttgctt taattctgac attcctgaat tcgctgcaat aattgatgca gaccgagcaa
     1021 tgtggggaga tcccgacgtt gagttttcct caatcaattg gacacatagc gaaccaacat
     1081 tttgggaagg atatggcaaa gccctaaagc aggattttgg cagccgcatc cgcagaacga
     1141 tatatacgct tttgtggagc ttactcgatg cttatgttta tttacaagaa tacaatcaac
     1201 cggaaaatgc taatgagaga aaaatcaaag ccttacacca gatagatata cttagcaccc
     1261 tactgcacat aaaataatca ggcagctgcg ttccataaga gtaaatccaa ttcaatattg
     1321 tttaagaaaa aggcttaatt tcatgtaatg aaattaagcc tttttttcgc agttctaatc
     1381 cttcaattta catattccaa gcagcattac caccgaaaaa acagttgcaa taagcagtcc
     1441 gtatttcaaa tttccgccaa gcacacttgc agcaaagcct acagctgtca gacctgctga
     1501 tgctcccata tctcctgcaa gcgccataac ttagtcaatt tcatcatact atttctcctg
     1561 tttcaagcgc tgcgggaagc tttgcttgtt aaacaactcc cgcacacttg gatatatttc
     1621 aacgaatttt ctgtactttt cctcatattt tgctgcaata tcaacgctcg gcgtgtatac
     1681 atctttcgtt ttcacgattt tatcagccgc ttcgtccaca tccttatact caccgaagcc
     1741 tacagctgca agtatagcag cacccattgc aggcccctct tcttttacaa gagttacaat
     1801 ctccatatta agcacattcg ccataatcga gcaccaaaga gggctctttg caccgccgcc
     1861 gcatattgtg gatctatgta tcttaattcc caaagttctt gcaacctcaa cggaatctct
     1921 tatagcgaac gctactccct caagcactgc ctgagtcata ttatttcttg tcgtgtccat
     1981 actcatccct ataaatgctc ctcgcacatc cggattatta tgaggagagc gctcgcccat
     2041 aagataaggc aaaaagaaga cattattttc accaaggttt tgtatgttct tttgactacc
     2101 ctcataatcc ttcgttccaa gtatctcctc aagccaccat ttattgcacg atgcggcact
     2161 aagcatacag cccatgagat gatatttacc gtcggcatgc gcaaaggaat gaagcgcgtt
     2221 attgttgtcc atcgaaaatt tatcctgtga aatgaacact gttcctgatg taccaagcga
     2281 aatattgcat ttgcccttgc ctaccgttcc tgtgcctatc gcagcggcag cattatcccc
     2341 tgctccggct ataactttca cactctgcgg cagtcccgtt tcctctgacg ctgcttttgt
     2401 aagcgttccg ataacatcac tgctttcata aagccctggc attactgctt cccttatccc
     2461 gcatataccg agcatttcct cagaccagca tcggtttttt acatctaaaa gaagcatacc
     2521 tgatgcgtcc gacacatctg ttgaatgtat ccccgtcaac ttataagcta tataatcctt
     2581 tggcagcatt atcttattta tacgtgcaaa attatcaggt tcattttcct ttacccacag
     2641 gattttcgga gcagtaaagc ctgcaaaggc aatatttgcg gtatattcag ataatgtttc
     2701 ttttcctatt atgttgttga gatattcgca ctgtttagta cttctgccgt cattccacag
     2761 aatggctggt cttattactt tatcgtcctt atctaacatt acaagtccgt gcatctgacc
     2821 accgaaagaa attccaccaa tcattgactt atcatagtct gctataagtt cttttaatcc
     2881 gtcttttaca gctatccacc aatcctctgg attttgctct gaccaaccag cttgggggaa
     2941 atacaatgga tattcctttg aaacactttt tataatttct ccattattgt ccatcatcag
     3001 catttttact gctgatgttc ctaaatcaat tcctatataa tatgccatag ccatagcctt
     3061 aaccccacgg attttcagtc gggtatctca cccccttcgg ctgattgtat ccaatttctt
     3121 caacaggaac gcccacatac ttaaatactt catataaagc ctttgcatga tgtccaaatg
     3181 ccactgcgcc gtgatgcgga taattctttt caatcaatac gtgacgataa aaacgtccca
     3241 tttcagggat tgcaaatacg ccgattgaac cgaatgaacg tgtcgcaacg ggaagcacct
     3301 cgccgtttgc gatgtaaccg cgaagcttag catcagaggt tgactgcaaa cggaaaaatg
     3361 taatatctcc cggcgctata tctccctcaa gcgttccgtt tgtaacctca acagggagtg
     3421 agcgagccat aattttctga tacttcatcg agcatgccgc aagctttgat gagcaggtat
     3481 ttccgcagtg gaagcccata aaggtatcct tatgcgtata atcaaattta cccttaatat
     3541 ctgcatcaaa catatccttt ggaactgaat tgttgatatc aagcagcgta accgcatcct
     3601 gtgatacgca tgctcctatg tattcgctga gcgcaccata aatatcaacc tcgcaggaaa
     3661 ctggtatgcc tcttcctgtc agtcggctgt ttacataaca cggaacaaat ccaaactgcg
     3721 tctggaacgc aggccagcat ttaccggcga ttgcaacata tttctttgac cccttatgag
     3781 cctcaatcca atcaagcaat gtaagctcgt actgagcaag ctttgaaagg atttcaggct
     3841 ttttattgcc ctcaccaagc tctttttcca tatctgctat aacctcagtg attctctcat
     3901 ctccttcatg ctcgttaaat ctttcaaaca agtcaagctc cgaattttcc tctatttcaa
     3961 caccgagctg ataaagctga taaataggcg cattgcaggc aaggaagttc aacggacgcg
     4021 gaccgaatga gataatcttc aagtcagaaa gaccgataat cactctcgca acaggcaaaa
     4081 attcgttaat catatctgcg cattcctctg cgttgccgac gggatattcc ggaatataca
     4141 cctttacacc acgaagcttt aagttatagc tcgcattaag cataccgcag tacgcatctc
     4201 cgcggtcatc tgaaagcgtg ccgatatttt cttcagcagc cgccacaaac atcttaggtc
     4261 cgtcaaactg ctgcgcgagc atagtttcgc tgatttccgg tccgaaatta ccaagataca
     4321 cgcaaagcga gttgcagcct gcttttttta tgtcctcaag tgcttgtttc atatgtattt
     4381 cgctttcgac aatgcaaaca ggacattcat atatatcatc tgcgctgtac ttgtctgcat
     4441 acgcctttac aagcgccttt cttctgttta ccgagagact ttcaggaaaa cagtcgcggc
     4501 ttaccgcaac tatacccacc tttacctttg gaatgttatt catttttatt acctcttttc
     4561 catttctata tttgatttct gttaaaaaac ttttctatat gattatatca ttataatata
     4621 tcaaactcaa tctttttctt gcatttgtta tatttttata tattttatac aatcattgct
     4681 cctacttttc atcaaatctc ataatcacag tatttttttt acttttttat tgacattcca
     4741 gcttaagtag gttataatac aattataaca aagttagtct ttgttatata gattgatata
     4801 acaaatttaa ttctggagg
//
LOCUS       DAWACS010000063         4799 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_1950_length_4799_cov_2.715219, whole genome shotgun sequence.
ACCESSION   DAWACS010000063 DAWACS010000000
VERSION     DAWACS010000063.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4799)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1950_length_4799_cov_2.715219"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1512 a          844 c         1064 g         1379 t
ORIGIN      
        1 ttttcacgtt tggaatatag ttgatggtga tttttgcaaa atccggctga cctgtgattg
       61 ggcaaagcga cgtaaattcg ggacagtcaa acgttaccat ataatcgttg ccttggtgct
      121 tgtttacgaa tgtttcaagt acctcaggtg catatgtgtc cttgtagact gtttttttgt
      181 tacctaaaag tgataaaccc tctgtttctt ctttgtttct cggcataata aactcctttt
      241 attccattaa ccatttcatt ttttgagagg ttgcgtgagt cataatttct tcaagctttt
      301 ctctgtcact gttttcgacc gcttcggcaa tctttccgag acttgcctgc atttcgtgta
      361 ttctgtttac aagctgttcc ttgttttcaa cgaaaagttc gctccacatt tgagcgttta
      421 taatcgcgac tcttgtacag tcacgcaaac ttcccgcact gaaaaacgtt gaacgttcaa
      481 tcataggatt gtcgcaaaga gcaacggcta caacgtgcat aagctgactt gtgtatgcga
      541 ttaccgcgtc atgttcttcc ggtgttgttg tgacaacgtg cttacagccg atatattcgg
      601 caagctcacg aataagagca atattttcgg gtttgttttt ctttgtcggc gtaatcagaa
      661 atgacgcttt gttgaaaagt gtgtctgtcg aactttgata accgccgact tcacgtcctg
      721 ccataggatg tgcgccgata aagtccacat tgtcgggaag tattttagac atttcatcaa
      781 tcataaaacc cttaacaccg gaaacgtctg ttataaccgc acccggtttg atgtgcgaca
      841 agttgttttt aaccatatcg acattaagct gcggataaag acaaagtatt attaaatccg
      901 cgttatccat tacttcgcct acgtccgaac ttccttcgtc tattgcaccg tcctgctttg
      961 caagtaagac agtttctgct gttcggttgt atgctgaaat tgtgcagttg tgaaaaccgc
     1021 gaagtctgcg tgcgattgaa ccgccgatta aacccaaacc aattattgct actttcattt
     1081 attttcactc cgttctacca taattcaaac tatattttat cacaaaattc gcaaattgtg
     1141 aatatatata ttaaaatttt atatttttac ctgtcggcta ttgacgatat atatgttgaa
     1201 atgataaaat attaaattga atacttataa tttgaaatag gagattagta tgtggagtgc
     1261 agataaatgg gagcagtaca gacttataga cagtgctgac ggagaaaaac ttgaatattg
     1321 gggagattat cttttaagac gtcccgaccc acaggcagtc tggagtttaa aatctgataa
     1381 aaaactttgg aataaggctg acgcttggta tcaccgttca aaatcgggcg gcggaaagtg
     1441 ggagtacttg aataagaaac ttcccgagcg ttggactgtt aattatcgca atttgacttt
     1501 taatataaag cctatgggct ttaagcatac gggacttttt cctgaacaag ctgtaaattg
     1561 ggattggttt tcggagctta taaaaaaggc ggacagacct ataagagtac ttaacctttt
     1621 tgcatataca ggcggtgcaa cagttgcggc acttgaagca ggtgcggaag ttgtacacgt
     1681 tgacgcgtca aagggtatgg taacttgggc aaaagaaaat gttgtgtcgt cgggacttgg
     1741 cgacagaccc gtaagatata ttgtcgatga cgttgttaaa ttcgtacagc gtgaaaacag
     1801 acgcggtcgt cagtatgacg caataattat ggatccgccg tcatacggca gaggtccgtc
     1861 gggtgaggtt tggaagattg aaaatgaact ttatccgtta attgaagaat gtatgaaaat
     1921 tctttcggat aaaccgttat ttttccttat aaattcatat acgacaggtc ttagcgcaca
     1981 agtgcttata aatgtactct ctatgaccgt aggcagaaaa tacggcggta aaatcactgc
     2041 cgatgagatt ggacttacga tgaaatcaaa taatcttgtt ttgccgtgtg gaattagtgg
     2101 acgctggcag gaacacagta caaaataaat ttaaaggaac aaattaaatg atagaaacaa
     2161 aaaatctttc ttttatttac cgtgaagaag atatggaaag cggagagata aaagaggaaa
     2221 aagttttaaa ggatattaac attgaaatag aaaaaggttc ttttaccgcc gtactcggtc
     2281 acaacggcag cggaaaatca actcttgcaa aacattttaa tgctatactt ttgccgtcgg
     2341 gcggtaaggt atatgtaaag ggtatggata cggctgacga aaataatata tttaatatac
     2401 gtcagtcggc aggtatggtt ttccaaaatc ccgataacca aatggttgcg gcactggttg
     2461 aggacgaggt ggcgtttgca cccgaaaatc tcggtgttga gcctaaagag atacgcagac
     2521 gtgttgacga gtgtcttgaa atagtcaata tgacaaagta tgcccaaagt tcaccgtcaa
     2581 agctttcggg cggtcaaaaa cagagagtgg cgattgcgtc ggtgcttgca atgaatcccg
     2641 aaatacttat acttgacgag ccgacagcaa tgcttgaccc aaaaggcaga agtgaagtta
     2701 taaagacgat aaaaatgctt aacgaagaaa aagacattac agttgtgctt ataactcatt
     2761 atatggacga agcggcacag gcggacagaa ccgttgttat tgacgatggt gaaattgtgc
     2821 ttgacggtac gccgaaagag gtatttaaaa atgttgaaaa attaaagtca ctcggtcttg
     2881 atgttccgca ggttacggaa cttgcgtatg aacttcgtaa aatggggata gaaatttcag
     2941 atgatgtgct gacggttgat gagtgttttg atgaaattat aagaattttg ggagaaacaa
     3001 aaagatgata aaagcagaaa acgttaatta tatatatcag cagggaatgc cttttgaacg
     3061 tcaagcactt tatgatgtga atattgaaat tgaggacggt tcgcttgttg cacttatcgg
     3121 tcatacaggc agcggaaaat caacgcttat tcagcacttc aacgcacttg taaaaccgac
     3181 ttcgggcaaa attattatta acggtattga cgttaccgct ccgaaagccg atttaagact
     3241 tgtcagaaaa acagtcggac ttgtgtttca atatcccgaa catcagcttt ttgaagaaac
     3301 tgtatataaa gatattgcat tcggtcctaa aaatatggga ttcagtgatg aagaaattga
     3361 taaaagagta agagaaagtg ccgcacttgt cggacttaaa gaaaagcatt taacgcgttc
     3421 gccgttcgac ctgtcgggcg gtcaaaaaag aagggttgca atagccggtg tgcttgcaat
     3481 gaatccgaaa gtgcttatac ttgacgagcc gaccgcggga cttgatccga aaggcagaga
     3541 tgaaatactt gcaacgataa aaaaacttca cgaagaaaac aaagaaatga taattatatt
     3601 cgtttcacat tcaatggagg acgttgcaaa aaccgccgaa cgtgtaatcg ttatgaatga
     3661 cggacacgtt gaaatgcagg gaacggttgc ggaggtgttc gcacaagcgg agcatttgca
     3721 gaaaatcggt cttaatgtac cgcaagtaac attgcttacc gataaactgc gacttgccgg
     3781 ttatgatttg cccgagcata tttatacggt aaaatatgcc gcggacgcaa ttaaaaaatt
     3841 aatcggaggt ggcggcaatg tttaaagata taataatagg acagtacgtt ccgggtaatt
     3901 caccgctcca taaaatgaat ccgcccgtaa aaataataat gacgatactt tacatagtat
     3961 tgttgttcat tttaaaaaat ccgatttcat atgtcgtatt tacgatttac acaataacat
     4021 taattcttat aagcggtgta ccttttaaaa tgatattaaa aggcttaaag ccaatgcttt
     4081 ggatatttat ttttacggcg gtacttaatg tgtttatgac gccgggtgat acggtgtggg
     4141 cactgaagat atttaagttt actttaaaga ttacaaaaga gggtatcgaa tccggttcac
     4201 ttatggttat aaggcttttg tatcttgtaa tgggaacgtc acttcttacg cttacaacgt
     4261 cacctttaca gcttacggac ggaatagaaa aactgttaaa acctttcaat aaaataaaag
     4321 ttccgtcaca cgaaattgca atgatgatga ctattgcaat aagatttatt ccgacgcttg
     4381 ccgaagaaac ggacaagatt atgaaagcac aaatggcaag aggtgcggac tttgaaacag
     4441 gaaatataat aagacgtgca aaggctatga taccgctttt ggtaccgctt tttgtaaatg
     4501 cgttcagacg tgccgacgag cttgcaacgg ctatggaggc aaggtgctac aacggcggca
     4561 ataaccgtac aaaaatgaaa gaaacgcata tgactaaagt cgatttggga gcaagtatcg
     4621 tatttgtttt ggcggctgct cttattatgg cggtagaatt tttaattgaa atttagtaat
     4681 ggaaatggta taatgagaaa tattaaactg aaaattcaat atgacggtac tgattatcac
     4741 ggctggcaaa tacagaaaaa tgacataaca gtacaggaaa cagtaaaaaa agccgttca
//
LOCUS       DAWACS010000064         4640 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2033_length_4640_cov_3.093348, whole genome shotgun sequence.
ACCESSION   DAWACS010000064 DAWACS010000000
VERSION     DAWACS010000064.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2033_length_4640_cov_3.093348"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2686..2695
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1092 a         1020 c          943 g         1575 t
ORIGIN      
        1 gtcatagaca tttacgctta cgccctcaac cgcttttccg tcattcgtct ttacaattac
       61 agatgtacgg ttatggtaat caatcttttt accgtcgggg aggcatacca taacgccgtt
      121 atcgtcaacg gttacaagcg ccatatatac cggttccgtt tcatcttcga caagtacgat
      181 atatccgttt acattcgccg taccgttttt gtccgtgtac gcctgatttt tattcggtaa
      241 tgtagcctga ccgtttgcgt ttgtaacgcc cttttctata tagtcgctgt cgcctatgac
      301 aatcacgttt acattttcct ttgcttcgcc gttctggtct ttgaccgtaa cagtaacgcg
      361 gttgtcgctg ttcatagccg taccgtcggg aagttttacg acaatatcgt ggttatcgcc
      421 tatgtgaatt tcacaatcgg gaatgacggt atcgtttttg tctgtaacgg taacaacata
      481 agtatatttt gtttcgccgt tatcgccgcc gatagtgccg ttttcattac ccgttgagct
      541 ctgattattc ggaacaataa gctgaccgcc tgcgtccgtc tgacctgttg cggcgttgcc
      601 gctcttgtct gaaatattga tattcaagcc ctctgccgct gcctttgtat ctgtacgaac
      661 aaccgtaatt gtcgttctgt cctcatagtc aagcaatctg ccctccggca gcttaattgt
      721 gatgttatcg tccttgtcaa tgctgatttc tgaattgaaa acaggcttgc tgtccttatc
      781 ggtaatcaga atactataat caccgacttt tgaatatccg tcctcgtccg aattgtcctt
      841 atcggtaagc tgggggattt cctcaatttt taaaactgta ccgcaatccg tacattcaat
      901 atgcctgctg ccctcattct caatcgtagc gggaacgtca ataatccaat ctgacatctt
      961 gtgtcctttt gccgatacct cgtcggctct atatgtttcg ccgcagtttt tacaggtgta
     1021 tgttgtatag cccaattctg tacaggtagg cgcggttact gttgtttcgt aatcgtgagc
     1081 ggtctttgcc tgctcgtttc cgatatatga aattccgcag tccttacaag taaaggttgt
     1141 atatcctata cccgtacaag tggaagttgt gattttttcc tcatagttat ggcctttcgc
     1201 gtccgtgtaa tccgcgacat acgattttcc gcaatcctcg caagtaaaag ttgtatatcc
     1261 catttccgta caagtcggcg cggttacagt gggaatatat ttatgctctg tcggctcctg
     1321 ctcgtcgccg atatattcct tgccgcaatc ggggcaagtg taaattgtat agccctgtga
     1381 tgtgcaagta ggcttaatcg tctgcttatt gtaattatga ggaagaacct ccgtataatc
     1441 agatttatag ctgtcgccgc aatcctcgca agtatatgtt gtatatccca tttccgtaca
     1501 agtcggggaa gttacaacag cattatattt atgctctgtt ttatccgtat aatctgaaat
     1561 gtagctgtcg ccgcaatcct cgcaagtata tgttgtatat cccatttccg tacaggtcgg
     1621 gggagttaca actgaattat acttgtgttc ggtcttatcc gtataattac cgatatatga
     1681 atggtcgcaa ttctcacaat tatatgtggt aaatcccata gctgtacaag tcggctccgt
     1741 tactgtttcg gaataggtgt gtcctgtcgg cagttcaaga atagcgccgc agctttcgca
     1801 cttttgcggt tcggtacagg tagcagcctt tccgggtgta tgtcctgtcg ctgaaattgc
     1861 ctgtatcatc ttttcatcac aatgtttaca gtggtattcc ataacgccct cgctctcgca
     1921 ggtcgaatta gttacagtgt gtccctcgtc ccatttatga ccgcgcggct cggtgtaatc
     1981 gccgacatag gtatcatcac aatttctgca cttgtatgtg gtaaagcctt ttgttttaca
     2041 agtgggcgcg gttactgtcg gtctgtaatc gtgttttgca agtggcgtta aatccgtaat
     2101 gtacttatat ccgcagtcga tacaaatatg ttctgtatat ccgttctttg tacaagtgga
     2161 ggcaacaata tttgtttcat aacggtgtcc cgtcattgag gtactttctg tataatagct
     2221 gccgcacttt gtacaggaat gtaattctaa accgccttgc tgacaactcg gctcacgaat
     2281 tacaacagta ttgtaatcgt gcccgcttgc gggagtataa ttcgttttct gcaaggctcc
     2341 gcactcggca cactggaaac ggtcaaaacc taactctgta caagtggggc gcactgtttc
     2401 caaaaatctg aaatcgtgag tatgaatact gtttgtattt gagggcggat tatcgcttaa
     2461 aagccataca tagcttacgc cgttttccgt catactctcg ccgccgtagg tatattctat
     2521 ctcataatat acgggataat agccctcgtc ggtataattc ggcgcgcttg tcttgttaca
     2581 ctttttcgct tccgttccgt aacgaatact tgtatttacg ccattctccg ataagtcatt
     2641 gacggttacg gtatgaggtt ttccgtcaac cttgccataa tagctnnnnn nnnnnttttt
     2701 cttatatccg cagtcctcac aatccgctgt aatgtgaaaa cgctgatttc ccaattcgcc
     2761 gtttacattt tcggtgaagt tgtggttttc ctgtttgtcc tgcacccgct ctttcgtgcc
     2821 tttgcagaat tgacaatact tgcccttttt tgcttttacc gtatgcgtcc tgctgttgta
     2881 cggtatgtat tccgtactgt caaaatccaa gaaaaagttg ctgtcgcagg ggttaaggga
     2941 atatacatct ttaccgcacg aatacgcgtt aattccgtca acggaattta ccgtaccgca
     3001 attctgacaa actgattttg tccaatgata ccccgtatag aacgcgtcaa cgccgggtgt
     3061 tccgtcgtca agattgcctt tgccttttcc gtcaataagc gtaccgtccg aatagtagac
     3121 gccgcgatta tatgcggttt cgccagttcg tgtgtattcg ggtacgcggt aggaaatgtt
     3181 gcttgtcggc atatcgcaga cggggcagaa cattgtaccc gtagttactg ttgcattgat
     3241 gtcaaactcg cttgtgcgcc cgtttgcttc aatccatacg tccgccggat cgacatattc
     3301 gttactctgc gccgcataag tggtgattga gggaataacg ctcaaagtca ttcccgccgc
     3361 cattacagcg gctaaaattc tttttatttt gtttctcatt tctaaaaatc ctccttgtca
     3421 agatttaaaa ttttgtatta aaaaacacgc tcgcataggc ggggcgcgtg attttgggtt
     3481 gtatgtaatt ttaattacct ccgtacatat cgtgatttac tttcaaggtg taatagctgt
     3541 caatagtgga gggggcattg aataacgccg caagtaaata ttttcttgta tcgtgtacgt
     3601 ctgttgtgtt attcttcata cagtctatga catattcgat atgctcgctg tttagtttca
     3661 gaagtttgga ttttaccgtt tccgcatttc gttcctcgct tgcaacggtt aagtattctc
     3721 ttgttgtaca taccgtttca gtgataatat caacaatttc attcagaata ccgatattgc
     3781 cgtttagctt ttgacataaa atatcatact caatattttg ttttataagc tgtctgtatg
     3841 ctaaaatctt atccattcct atccgttcag gctgttgtgg ttttacaggt tcgccgtttt
     3901 cctccggctc cgtagaattg attggataag tatttaataa atcagtattt gatatttcag
     3961 tatttgattt attagtattt aattgcgtgg acttgccctg ttcaggttca gcctgtacag
     4021 gattatccaa tgttggattt tccaatgtag gctcggtctg ttttggttcg ggcgatatag
     4081 gcaattcgtg tatgatatat tcattttcgc caagctgccc tttttcgttt ctgacgcggg
     4141 aacgctcaat atatccaaac gcttcaagct cattgattgc cgagcgtatg gcgtcaatgc
     4201 cctctttatt taaagttgct aagcctttaa gggaataatc ccaatcttca ggcaagctaa
     4261 gcataaacga taaaagccct tttgaccgca gggacaaatc tttatttttt aaatgatgat
     4321 tagacatcac tgtaaaatct tttgttttct gaactctgaa tactgttgcc atagctgata
     4381 tgctcctttc ctaaatatcg acatcaaaaa aagacattga gtgttcccaa tgccttatat
     4441 ataaaatatt ctgttttact tcggcaaaat aaaaattttg tcgttctttt gttttttaat
     4501 tgaccgcctt atgtatttat gatatactat ctgagaatgt aaaggtcggt ctttggtgca
     4561 aaagggaaat aaaaaatacc gtaaatatgc aattcacgat attttttaaa cataaaatta
     4621 ttttatttta aggttaatac
//
LOCUS       DAWACS010000065         4607 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2053_length_4607_cov_3.173111, whole genome shotgun sequence.
ACCESSION   DAWACS010000065 DAWACS010000000
VERSION     DAWACS010000065.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2053_length_4607_cov_3.173111"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1460 a          740 c         1098 g         1309 t
ORIGIN      
        1 taacactgtt tataacatca tagcgtcgga ctatatgaaa gcaatgtaca caggcattga
       61 tacaataaaa gttgctgtag cgattgcggt ggggattgta cttgtgtttg cattgccgaa
      121 taaaatgttt ttcaagagaa aatagttcca atgggctttg gcttgacata actgatattg
      181 tatagtacaa taggcaagat agaagtaaaa attatacacc gcatttttac ggtgcagtag
      241 gaaaggattt tacgatagta atgaaaaaga taattgccgt tatggcggtg cttataacgc
      301 ttttttcaat gggaaatgtg tcggcaagtg cggcggcaaa ggttgcggca ctgatgtatc
      361 acagtgtgac aactgattcg tcacgttgga atgactatac aatatcaccc gagcagcttg
      421 atgcggatat acaatacttt aaaagctgcg gatatatacc gatgaccgcg acagaactcg
      481 caacggcgaa tatggctgat attgacaaca gaaaaatatt gcttttgaca tttgatgacg
      541 gatactcaaa cttctatacg gaggtatttc cgatattaaa gaaaaatgac gcaaaggcta
      601 caatgttttt gattggctca tacatagacc gttacggtta tttgactgct gacgaaacat
      661 atgaaatggc gaacagcgga cttgtcgaaa taggcaatca cacaaacggt attcacagta
      721 tgcctaagga acagttaaag gcgatttaca acaatacaaa cgcttacggc gatatactcg
      781 ctgatattca gagtaacgga gaaattttga aagaagttac tggaaaagat gtgacaagta
      841 tttcgtggcc gtacggatat tatacgacaa cactcgataa tgcggttaaa agtcagcttg
      901 gttataagat ttcattcagt acggtgtacg gtgtgaattt ctttaccggc aatacataca
      961 gtccgttaaa acgtatgaac agagaatact ctgccggaac tcaacaggtg tttgacaggg
     1021 caaacggtaa attttattaa tcatcagcgg tacggtgaaa agtaccgctg tttttataaa
     1081 tgaggggtag tatgatatga atagaataga gattaaagat ttttcgataa aaatagataa
     1141 agataaagta ttaaaaactt tggggtgttt tgaggggagc agtgtgtatg aaactgtttc
     1201 ttcttatttt gacgagcttg aagaaacggt gatgaattta ctgtccccac gtgcagttgc
     1261 cgtaacggag gatatgaaag catattgcat acttacggtc ggtgaaaaaa ttagcggaat
     1321 atcaaaaagc ttttttgata acggtgaggg tatgaaaggt atacttgttg acgcaatggc
     1381 ggacgagtat ctttttatga tggatgatgt gcttgccgaa aatataaagc ttttgtgtgc
     1441 aaaaaagtct tggggtgtta aaaaacgtct tgatggaccg aaagattttc cgctttctca
     1501 acaatccgtt attgtcgcga aaacaggcgt tgacggaata aaaatgacaa gcggttttat
     1561 gtttgaaccc gtaaaaactt ttggctacat acttgaattt acgactgatg aaaaggtatt
     1621 taatgcacag catgactgtt caaagtgttc taattttgac tgcccgagaa gaagtaatat
     1681 aaaaaacggc agatttgaag tgctgtcgag ttatgagtat aaaccgaatt ttaaagaggg
     1741 tgacagtgca gtctgtattg atataggtac aactactgtt gcttttgaac ttgtgacgga
     1801 taaggggaca ctaaaaacat acagaacaat taatcctcaa agacgtttcg gactggatgt
     1861 tctttcaaga atcgagtcgg caaacagagg aaggttggac gagctttcgg ctgttatgcg
     1921 ttatacgatt ataagcggat ataaaaaagt gaccgaagaa tttggggaca ctaaaaaagt
     1981 tgttatcgcg ggcaatacaa caatggtaca tctgcttatg ggatattctt gcggtacact
     2041 cggtgaatat ccttttaaat caaaacactt ggggacacta aaaactacgc ttgataaagt
     2101 tacaaaaagt aaggtttcgc cgattgaaac cgttatatac ggcggtatat ctgcgtttgt
     2161 gggcggcgat atagtgagcg gactttatat gagtgatttt gataaatcgg ataaggttaa
     2221 tatgtttatt gacttgggta caaacggtga aatggcgttg ggcaataagg ataaaatgat
     2281 tgttacgtct actgccgcag gacctgcttt tgagggcgga agaatttcgt gcggaatagg
     2341 ttcggttgac ggtgcggtat gcggtgttga tttaaaaatg gggacactaa aaactattgc
     2401 ggataaaccg cctgtcggtc tttgcggaac gggtataatt gagttggttt cggaattgct
     2461 tgacgaaaaa ataattgata aaacgggtct tttaaatgac gactatttta taaacggtta
     2521 taaggtcgcg gaggacgttg tgtttacgca aaatgatatt cgtcaggtgc aaatggcaaa
     2581 atcggcggtt agagccggta ttgatgtgct tgcgaagtcg tggggaacag agctttcaca
     2641 aattgatacg gtgtatcttg ccggcggttt cggttacggt ctttcgattg aaaaggcttg
     2701 caatatcgga atattgccgc gtgagttttt gggtaagacg aaagttatcg gcaacagctc
     2761 gctcggcggt tgcgtcaagt atgccgaaag gcaggacggc gatgaaagaa tcggtcgtat
     2821 aaaggaaatc agttccgaaa tttcactcgg aaacagtgaa gattttgaaa aattgtatat
     2881 cgaatatatg aatttttgaa agaaataaaa agttacgcaa aactaacaac ggcatagtta
     2941 agccatattc gaaaaacatc aatatttttt tgaaaaaaat tcaaattttt tgttgcataa
     3001 tcataaaaat aatgctaaaa tatatgttag attggcacaa cagagtgaac catagcaaac
     3061 attgtacact ctgatgagaa aaaaattaat ttttagagga ggtttaacta tgtcatacgc
     3121 acaagacgta ctagcaaagt taaaagagaa aaacgcaagc gagcctgaat ttatacaggc
     3181 ggcaacagag gttctaacat cacttgaacc tgtatttgag aagcatcctg aattcgagaa
     3241 ggcttcattg cttgagagaa tagtagagcc tgagagacag attatgttcc gtgttccttg
     3301 ggttgacgac aacggtaagg tacaggtaaa cagaggtttc agagtacagt tcaacagtgc
     3361 tattggtcca tacaagggcg gtctaagact tcacccatca gtaaaccttg gtattatcaa
     3421 gttccttggt tttgaacaga ttttcaagaa ctcacttaca acacttccaa tcggcggtgg
     3481 taaaggcggt agtgacttcg atcctaaggg taagtcagac agagaagtta tggcattctg
     3541 ccagagcttt atgacagagc ttttcagaca tatcggtgct gatacagacg ttcctgccgg
     3601 tgatatcggt acaggtgcaa gagaaatcgg atttatgttc ggtcaatata agagattaaa
     3661 ggacgtatat gaaggcgttc ttacaggtaa aggtttaact tggggcggtt cacttgcaag
     3721 aactcaggct acaggctacg gtcttgtata ctacgctcag gaaatgctta aggatttggg
     3781 tactgacttt aacggtaaga cagttgctat ttcaggtgca ggtaacgttg ctatttacgc
     3841 ttgtgaaaag gcacagcagc ttggtgcaaa ggttatcact gtttctgact caaacggtta
     3901 cgttgttgat gaagaaggta tcgaccttgc agcagttaag gaaatcaagg aagttaagag
     3961 aggcagaatt aaggattatc ttaactacag accaaatgct aagtatgttg aaggtgcagg
     4021 tctatggcaa gcagttaagg ttgatgttgc tcttccatgt gctacacaaa acgaattgct
     4081 tattgacgac gctaaggctc ttgttgcaaa cggtacaatg atcgttgcag aaggtgctaa
     4141 tatgcctaca actcttgagg ctacacaata tctacaagaa aacggtgttt actttgctcc
     4201 tggtaaagca tcaaatgccg gtggtgttgc tacttcagct cttgaaatga gccagaactc
     4261 acaaagactt tcatggacat ttgaagaagt tgatgaaaaa ctacataaca ttatgattac
     4321 aatttacaag aattctgttg ctgcagctaa ggaatataac tgcgaaatcg gcggaaagac
     4381 taaccttgtt gccggtgcta atattgccgg atttatgaag gttgctgacg ctatgatggc
     4441 tcagggcgtt tgctaattat aatattttga attttaaaag gtgtttccaa tcggaaacac
     4501 cttttttgta ttggttgatg tattttattg tattatgcca cccctcagtc aactgcgttg
     4561 acagctcccc tcaaggggag cctttatgaa gtttatcggc aatttta
//
LOCUS       DAWACS010000066         4537 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2085_length_4537_cov_2.071397, whole genome shotgun sequence.
ACCESSION   DAWACS010000066 DAWACS010000000
VERSION     DAWACS010000066.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2085_length_4537_cov_2.071397"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1612..1621
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2553..2562
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3181..3190
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1554 a          778 c          994 g         1181 t
ORIGIN      
        1 gtattttagc atctgacagg aaagtttcac ttggaaagat gagtttccaa atcgtagatc
       61 ctgttcaagt atgtaatatg acgcaaagtc aacttaatag tattttaagt atagaagatg
      121 atagtgcgta tattatgtat gtcggagatg accatattga atactttgta gatgtacttc
      181 cggcagaatg gaaagttgtc agaacacttc ttgatgtaac tccggcagtt gaagaacgaa
      241 cagttactgc gtattctatt tataatagat gctcagatgc gacaaaagcg ggtacattgg
      301 cagatttggt tgcatatttg aaccaaacaa tgaataccgt tgttcagaga tattccgacg
      361 gacagcaagt gctgggcaat atgatatgga actccgaaga tgaaactttt acagttcaat
      421 atagcaatgg aacagagtat aatcctttgg gtaatgtgac ttatacggta agtcaaaact
      481 atgcaggtac agataaagtt gtaacggtaa aagatacggt aaaacctgtt acaattaccg
      541 gctttaaata tgataatcgt attttgtcat acagtgacag taacagaccg gaaatttggg
      601 atgatttaga aatgcctgat actgtaactc ctgttttgga cggcattgac gatatgtatg
      661 tttctccgac tcaaaatcct atccaaacgg attggacgcc tgatgatgtt acggaagctt
      721 tgaaaacagg tactgcgcct gtcagtgaaa catatacaga agaatttgac aaaacattgt
      781 ttggagcacc tgagccggct tggcttacaa ttcctgacgg atttaattgg aatattgacg
      841 cgttgagaaa tgtattaaag ggaaatgatt atctcaagga tgcaacaata actgcggaag
      901 ttgaaagaga aacgggtatc ttagatataa cggttactcc taaagacgga gcggcttttg
      961 aagacggaac aaactttaat atatacctgc caaacggaat ggtattaaaa agtacagata
     1021 acaatgattt tgtaacggtt agtattgtta acagacaggc agttattaaa attaatgcga
     1081 tgtctgacag tgcaacgagt atgacgaata cagaccgtga attgatacaa agtatcataa
     1141 acttgggtaa tgatgatttt aaattgtcag caattcctcc agatgatgca gaaactgcac
     1201 aagtaggttt tgtgtttgat ccgagagtta attattatct tggtgaaaac gaaaataact
     1261 atgtcgaaaa agactattca gagggtagag ctggtatgtt ctctgtatac gagggacaaa
     1321 gtcttgcgga tatagcaaca tatatatcat tccctgatag aagtacaatt cctattgcat
     1381 atcatggaca gaccggttat cagccgtctg aatatgacag tgcaaaggtt gtaagttgga
     1441 gcatcgaagg tgatccgacg gctactgtgt tgccgactga gggaacaaca gtaacactta
     1501 tcggtaaact tgaagattac tcatatacaa atttcggata tgtacaaaat ccggacgata
     1561 tttacttaaa aataaaggtg acaacactgg caattcagcc tacggtaaaa cnnnnnnnnn
     1621 ncctactgaa tcgccgaacc cgagcgagtc gccaaatccg agtgaatcac caaatccgag
     1681 tgaatcaccg aacccaagcg agccaccaaa tccgcgtgag tcgccaaatc cgagtgagtc
     1741 gccggcgccg agtacatctc cgactccgga accggaaaaa gaggcaataa cgattactac
     1801 tgatgtagga agtgcggaca gtccggtaga agttgtgctt aacagtaaaa cgtttgagta
     1861 cgatacaaag aaagtgggtt attcaactgc cgatgtacaa agacaaatgt acacgcttga
     1921 gaatgtcggt atagataata taaccagtct gacaatgaga atttccgatt tcgttcagac
     1981 tgatggaaca acggctacag acagtagtcc tacaagcttt gtaaacagca taccattgta
     2041 tgttactttc ttgaatgttg gcgacaaaac gcagtttgaa attcgtacaa agcatggctt
     2101 ggcggtcgga acatattcgg cgagagtcag cataggctca aataaaaatg ctgaattggc
     2161 atatttcaat ataaccttta aggtaacgga aaatgaagtg tataccgtaa ctgttgacaa
     2221 cggcgacagc gtacaacaat ctatcggatt gggatatttg atggacgaaa caagcaacag
     2281 gatatgttca agaacatatg aagtaggcga aactgttccg atatatgttc agattttgga
     2341 ttccggatac acattcgata agtgggaaac tgatgatggt ataacgttcg caaacgcatc
     2401 tcaaatgtca acgacattta cgatgatcgc gcaaaatgta acggtaagac cgacttatcg
     2461 tgaaacaaat gatgtgtggg ttcgtttggc ggacttgagg gattataatg aaaatgagac
     2521 aatgccaaac gatttaagag agtcacttcc acnnnnnnnn nnacatttac cgaaacaaca
     2581 tacgcataca gagtaattgt agacggctct gtagaaaaga attttgttga attcgatttg
     2641 aagaataaac tgttggaatt aactccgtct atgacggtga cagcaacagc gaacggaaat
     2701 acattgacaa tagcgcctca agttgattct acatcaacga gtgttacgac atataaatcc
     2761 gacttgtttg atcttcaaga aggacttaat acagtcaaaa ttaagacaga atatacagac
     2821 ctgaatgacg gacaaaatta ttcggctgaa tacacgatta caatactcag aaaaaaagcg
     2881 gttgatgttg atataaaacc gggtaattca ccttacggtt taatcgaatc gtccgacaat
     2941 ataacggata agtcaacggc aaaagatttg ttctcgaaaa atcatacata tgatgcggca
     3001 tatacaccga acaaagcggt aaatacatat gatacaaaat attatacaga tgcatgggac
     3061 ggcgtgaatt acgatgaaaa tccttatacg ctgtttgtat acaacggaca aacgtttgtt
     3121 gatccgggtt ttaaaaatct gaaagatatg gacggaaata cagtttcggc ggaaaatgta
     3181 nnnnnnnnnn taactcgtac aatagaagtt caaactgtca atgttccgac aaatgtaaca
     3241 gatataatga caacaacatc gcaaacggtc actgtggcaa atacaggagc aagttgtgtt
     3301 ataagtgagt tggcaagtct taatataaga ccgggagtgt atacgttaaa atatacattt
     3361 accgatactg ataatacaac acgattgttt acaagacctg tagtggttct tgcgaataaa
     3421 ggtgatatta accttgacag aactgttgat tcaaatgacg cggatatgct ttatcaaaga
     3481 atgaataacg gtttctatac cgatataata aattctgctg atgaatgggc gaaaatctac
     3541 gcatacagag ttgcagatgt caccgaagac agaaatgtca actcgataga tgctaacgca
     3601 ctgttgaata taatgcctac accgtattac gaatcacttc cgacaacggt tagtgacagt
     3661 atggcgacat tcgaccctga tacggcggtc agacaaccgg ttgcgacagt tccgccggaa
     3721 aaggcaacgt taacgcttga ctatctcgga actgcgtcaa aacctttgga caaacctcaa
     3781 acaccgaact tgacagcgga taatgtctat gatgtggata atgccggttc aggtattgtg
     3841 tggtttggaa taagtataaa aaatacgcag aatttgaagt atttccttga cggattgtac
     3901 agtatggatt ttgcgataga ttatgacagt gaaatattca ctccttgcga cggcaatatt
     3961 agaatcagta ccgatacggg ctttgacctt gccggaacga ttgaaagtaa caatatcaag
     4021 tcagatgtaa cgacgagtga tgtggcgtat tggaagaatg cggaattgta taaacaatcc
     4081 ttgcaaagtg attgggatat tgaccaaaca gacaaatata aaacggaatt tgttacaata
     4141 aaatcagtcg acggtacaaa tttgcgactg agtgatttta tacagactga tggatatgac
     4201 agagtttatc tgcttagagt gccgttcaga ctgaataaaa tgccggagga tggttatgtc
     4261 ggcaagccga taacactaaa ccttacggaa caaacatttg taatgggcgc aaccgaaaac
     4321 ggtgtaacaa attcagcgtc atgggaaggt gacgtggata aaaccacaga agtcaataat
     4381 gcaaagaacc attttgacgg tgttgaaata gttgatatat tcaatactga cggtaaatat
     4441 aatataatcg gtacagttaa gtgttggaat gatacaaaac cggttacagt tgaaatatac
     4501 aaagacggtg aaacgacacc gatgtataca tttacat
//
LOCUS       DAWACS010000067         4518 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2098_length_4518_cov_2.217343, whole genome shotgun sequence.
ACCESSION   DAWACS010000067 DAWACS010000000
VERSION     DAWACS010000067.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2098_length_4518_cov_2.217343"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2134..2143
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3559..3568
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1483 a          733 c          986 g         1296 t
ORIGIN      
        1 gtttagggtc gatttttacg gaataaacaa tttcaccgct gctgttacag acagaatatt
       61 caccgtatgt tttcaagttt ttctttatta taaattctgt ttgattttcg gtatttaata
      121 tatatgattt aaaaaatatt ttctttatct tcaattccat aaaacaacaa ctccgttcgt
      181 aaaaattgtt tttataaaat tatataatat atacggttaa atgacaatac aaaaaaatta
      241 aaaaaatttc ctatattaaa ttgacaataa aaaaaatata tgttatgatt aaacctatcg
      301 gataagtatg atataaaaat acggtgtgag aaggggatta tatgtacaag gtattgcttg
      361 tttttaagga tgaaaatatt tctgaaaaga taaaaagaat gaatatttgg ggcgaaaatt
      421 cggaatttga aatatcggcg gtcgcaaaag acggtgcggc ggcttatgat gaggttagaa
      481 aacagcacta tgatttggca gtcatgcaaa cggatattga cggaatggac ggacttcagc
      541 ttttaaggca catcagaggt gaaaggctgt gttcgtgcgt ggtgcttttc agcagtgagc
      601 cgaattttga aaacgcaaga cagggtataa tttacggtgc gtttgattat cttacagagc
      661 ctttgactga aaagtcgttc tgttcgattt tcgaccgcat agagaacaga actgacaaga
      721 aggaagaagt ttatcattcc gaagaaatac tcagttgtat agaacagcac ggcggcaatc
      781 ttgaaaaaca gcttgccgat acacgcactg atatatacaa tattgctacc gataacgtgc
      841 ttgcggaaaa tgcggttatc agcacatacg acaatgttgt aaacgaactt ttcaagcaaa
      901 acgaatggct tgatttgtat attccgcata ttgatgtcaa ggagtatgtg gggatagaga
      961 gaaccgagga cgccgaaagg gcggttatcg aaactgcaaa cgaagtgtgg gaattatatc
     1021 ccaaagttca aaatccgcaa atgcaggatg ttatattata tatattgaac aatccggaaa
     1081 gtgacctgaa acaaaagact attgcgtcta agctgtatat gaacagttcg tatttaagta
     1141 cggtgttcgc aacacagaca ggtatgcgct ttgtggatta tatcacaact gtacgcttaa
     1201 aacgtgcagg gtggttactg aaaaatacgg aaatgaaaat taccgaaatc gcattaagac
     1261 ttgattataa ggacgtcgga tatttttcgc aacttttcaa aaagcaatac ggcattgttc
     1321 cgtcacagta caggttgccg gataattata attatcagat ttaaacttgt ataatgacgg
     1381 atttaatagt ataatgttac taaaaaagaa aaaaggatcg aattaatgaa actgaaaaat
     1441 atattgattt taatactttg tatagtgctt tgtccgccga tagtaatggc ggtaagtgac
     1501 aatacggtgt acaccgaatt taccggcgga aacagttcga gcacaggaaa cggaacagaa
     1561 caatcgcctt ataatctgtt tgaggacgca ttaaatgccg ttgaggacgg cggtacaata
     1621 tgtgtcggcg aaaaaggcgc atttgtaaac agcagtgacg ataaaccact tgtaataaac
     1681 aaaaatgtta caatcacttc aaaaagcgat accgcaccgg agatttcaat acgcaaagcg
     1741 ggtgtggttt tgggcggaaa cgtgagtttt aaaaatgtgg ttttgtcgct tgtaaacggt
     1801 aatcacgcac tgattgcggc aaacggatat acacttacgc ttgacaatgt tacatatttt
     1861 caaaatacaa gggaagtaca tattgtaggc ggtacgttgt acgacaaaaa cggcgtgagc
     1921 ctgagtccga cggtcggaga aaaaagcaag atagttttaa gcggtaataa aacgcatttc
     1981 ggaaatatct atgccggaag tatcaacggc agttttgata aagatgttga aattgatata
     2041 aacggtgtga cgggtaaaaa tatcggtaag gtgtattcgt gcggagcgaa agaaggttat
     2101 tataattccg ataatttcct tgatccgaat aatnnnnnnn nnncgagata gacggcgact
     2161 gcgggagcgg cagagcaaac gtcagtgtgg taacggaata tcagtacagc agtgtaatga
     2221 aaaatatagg tttgcttacg gttgacagcg gaatgattga gcttaccgag attaatgacg
     2281 atgtcaacgt aaagattaat tcaaacggaa ttttggatat gtcaaacttg ggtgaatgca
     2341 gtgtaaatga tttttacggc ggaggtacac ttgttttggc aaaggacgga ttgcttacgg
     2401 taaacggtac actaagcggt gtgacagaat ttcaaacttc gggcggagta aattcttcgg
     2461 gtattgccga gtatgacagg ttatatatta agacatcaaa gggtgacgga agttttactt
     2521 tcaatccgta tgccactcaa tctgatatga cgcttgacaa gacggtggac ggctttaaga
     2581 cgagcagtaa aacggaagaa gctgttgttt tgaaaagttt tgggataaca gggagtgaat
     2641 ttaatgacac ttcgcttgaa gttaatcaag gttattttga tattccgatt gaaacggaat
     2701 ttgagggcag cggtttttat gatataagta tggtaccgct tgtttatact gtttcatata
     2761 atggtaaaac gtacggcccg agaaattcaa cgttgacaga tgacggaaat tatgaggctg
     2821 attttaagga acttaatatg tgctttgcac ctataacggg caatataagc gtttcgaaat
     2881 attcgtcaac gattggattc atggatgata ttgcagaggg cgtgtatgat gtaacggtta
     2941 ctgcaccgac tgcaagcgga aatgttgaac gtagctttaa actgattgtc ggagttgtga
     3001 caccgacagt aacaccaaca gtcgaaccaa cggcgacacc gacagttaag ccgacggccg
     3061 aaccaacggc ggcaccgaca gttaagccaa ccacagaacc gactgcaaca ccgacagtta
     3121 agccgaccgc cgaaccaact gtgacaccga cagttaagcc gaccgccgaa ccgacggcgg
     3181 caccgactgt aagcaacaaa gttgagttta caaaagatgg tgacagggtt aatgcgaaaa
     3241 ttatattaga caagcaagtg tcggcggaaa atataaaaat gtatgttgta tacaaagaaa
     3301 atgatgtttt gaaacgagtt gaaacggtga acgtaaatga tgattttacg gcagaattta
     3361 caataccgaa tgaactgaaa aatgcagaaa tatcggttta tatatggaat gataaaatga
     3421 acccgtatat ggatatacaa caaataacga gcctggcgac aaaatgtcgc cagctccaaa
     3481 aatcacatct cgcgatgtaa tttttgagta tttttagaga aaaaatcata aagttcctcc
     3541 aaagtcgaaa ctttagatnn nnnnnnnnaa agtagacaaa accataatgg ttttgtctat
     3601 aatctcaaat gacgaccgag cagtcgtcac ttttttaatt tgatgaatat ttgtttattt
     3661 agtgcgaaaa ttccgcattt atatttgaat ttcatacttg tatttttaaa aacaagtttt
     3721 cggtacatca aacgaaattg tttataaagc tctttgattt tatcgttttc tcctaaaatg
     3781 ccgtaacctt taatgatact gtacattgcc atagaaagag ccttttcggc gagggtgtat
     3841 tcttttttgt taaagaaaaa ttgacaacgg tcaagcatac cttcggcggc gtttaggttt
     3901 tcggttttaa actgcgtgtg cataatactg ccctcacgct gaagataaaa ataaagaggt
     3961 ttttttgaaa ctgcgatttt actgcattga cttatgattt gatgtacggc aaattcgtcc
     4021 tcatgcaacg tattaagcgg aaaacgaaca ttattccaaa gatgactttt atataacttt
     4081 gcccacaagg tgacgtaata aattcttgtg tcggtgtaaa aataattcca aaatgtatcg
     4141 gttgatataa ttccgtctgt gacagttacg ttttcctgcg gagagagatt gccgttttcg
     4201 tctacactgg tgtaaccgca cattgctata ttggtatcac cgatattttc tgaaagaaac
     4261 tcaatcatat tcggtgcgat ataatcatcg gaatcgacaa acgttatata atcgccgtgc
     4321 gtattgtcaa gaccgatttt tcttgctgaa gacacacctc cgttaggctt gtggattacc
     4381 gcaattctgt tgtctttttc ggcaagactg tcgcatattt gcggtgaatt atcagttgag
     4441 ccgtcatcaa taagtattat ttcgagattt ttatatgttt ggttaagtat gctcataaca
     4501 catttttcga tatatttt
//
LOCUS       DAWACS010000068         4399 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2163_length_4399_cov_2.819291, whole genome shotgun sequence.
ACCESSION   DAWACS010000068 DAWACS010000000
VERSION     DAWACS010000068.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2163_length_4399_cov_2.819291"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1376..1385
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3344..3353
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3630..3639
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1411 a          809 c          978 g         1171 t
ORIGIN      
        1 gccatacata gttgaagtgt attcgtacaa tgatgaacga cacgaatata taatggaact
       61 tatggatttt actttagaaa aatatatcag cattaataat tccaatataa cgatacaaac
      121 gcgaaaaaat atcattatgc agctgttacg agcatacggt tatttgcatt ctaaaagtgt
      181 ttgtcaccgt gatattagtc ctaaaaatgt tttattaaaa cagtatgatg atgtattagt
      241 agtaaaatta tctgattttg ggttggttaa aatcgctgat agtgacttga cttctgaaaa
      301 tacggaattt aaaggttcgt taaatgatcc ggctctgaaa gttgaagggt tcggaaacta
      361 tgaattggca catgaactct atgcaataac attgcttttt gcatacatat taactggtaa
      421 aagcaattgg gcgaagataa cagaacctgt tgttaaatct tttatggaaa agggaacaaa
      481 tccggataag acgaaaagat ttcaaaccct tgatgaactt ggtgatgcta taaaaagatg
      541 cattgcagcg ctggaataat agcgaccaag ctaacggagg caaaaacaat ggtaaaatta
      601 caggaattca acggcaaata ctgtgagtcg gaatatgaat atgcctttct atcattttta
      661 gaggatgagg gatggcatta tctgcacggc aacagtattc cgcgcgattc aaaaactacg
      721 gttttatatg ccgatgatat ggaacaattt ttgagcaaaa ccaacacgga tcttactgct
      781 gatgaaatcc atcagattat ggataccgtt cgtcttgtag gtgcggaaag cgagtttgcc
      841 actctgcaca aggtgtatgg ttggatggtt ttcggcatac agtttacccc tcaagatggt
      901 attgcccgtt tgatcgacct tattgatttt gaacatcccg aaaataatat cttccgtgca
      961 gtaaaccaat ttgctgttga atatatcaat aacggtcaga aggaaacccg tcgtccagac
     1021 atccttctgt tcgttaacgg tatgcctctg tgtatcattg agcttaaaaa tcctgcagat
     1081 gccaatgcaa cgatttatga tgcttatgag cagattacca ctcgttattg gcgcgacatt
     1141 ccgcatcttt tgcattattg ccctttggcc tgtatttcgg acggcgtgaa aacccggctc
     1201 ggtacagttc gtactccata tgagcatttt tatgcctgga gaagagtaaa tgacggcgac
     1261 gcggtttcaa cattgccttt tgaagaaacc gaaaatatga taaagggtgt ctatgcgcca
     1321 gaacggtttc ttgagatatt tcgcgactac atctattttc aggacagcat ttacgnnnnn
     1381 nnnnncgaca gcgacgagcg cgaaatcgta tgccgttatc ctcagttttt tgccgcaaga
     1441 cttcttaaac agagcattgt aaaatccgtt gaagaaaaaa gcggaaaggg cggtacatat
     1501 ttcggcgcaa caggctgcgg caagacttat accatggctt tccttgcccg tcagctttca
     1561 atgcgctgtc ccgagcttgg ttctccgacg atcataatga tcgttgatcg tgatgagctc
     1621 caaaagcaag gtgcaaagct atttacgaaa agcaaagagt ttttgaacct tggtgaagta
     1681 agcgttgtcc ccaatcgcaa ggttttgcgg gaggaattaa gctcccgtaa aagcggcggc
     1741 ttctatatat gcaccattca aaaattctgc gatcgcgagg acgacaaaat cggacttctc
     1801 aacgaacggg cgaatattat ttgcttttca gacgaagctc acagaacaca gcttgaacat
     1861 tctaaaaaaa ttcagttcag taaagatgcc gacgaaaata tgaaagccat ggtttcaaag
     1921 ccctatgcaa aggtgttgaa agaggcgttc ccgaatgcaa cctttgtcgg atttacaggc
     1981 acacccattg ccgaaacata tcagacattc ggtgatgaaa tcgaccgcta cacaatggat
     2041 caggcggtag ccgacggact gacggtttct atcaaatatc atccgagaat tgctaaggtg
     2101 ctctttgatg ccgaaaaaat caaacagata gaaacatatt ataaaacatg tgctgatgac
     2161 ggtgcaaccg aggaagatat tgaggcaagc aaaaaggcta tgagctctat ggaagttatc
     2221 ctcggtgagc cgtcccgctt ggagcgcctt gctgcagata ttcacgatca ttatgtatcc
     2281 tcctgcgcca aagaccccga cagagtgcag aaagcaatga ttgtttgctc aagcagaaca
     2341 attgcttatg aacttctcaa aaaatttcag gacaagtatc cggaatggtt tgtagaaaag
     2401 aaggtcgctg acggatttac ggcaaccgat gaggagttga aagaattaac gccgatgcct
     2461 tttatggcta tggtcgccag tgttggcagt aatgacaaag cagatatgta caattacctc
     2521 ggcggcgtga aaaacgataa gcgctccgag gctttggatg ccgcatttaa acaggataaa
     2581 tcaaatttcc gcattgtcat tgtagtcgat atgtggataa ccggttttga tgtgccgtcg
     2641 ctcacatata tgtacaatga caaaccgctg aaaaagcatc tgctgattca gacgataagc
     2701 cgcgtaaaca gaaaatatcc cggaaaagag tacggaatga tcgtcgatta tatcggtatc
     2761 cgcaacaaca tgcgtgaggc aatgaagata tacggcggca acacatcggt tgcgcctaca
     2821 acagatgatg tcgaacaggc cactacagta tttagggagg aactatctgt tcttaacaat
     2881 ctgttttccg gatatgattt aaagccgttt ttgaatcccg acggtgatcc gtttgagcgg
     2941 tacaagcttc ttgcgaaagc ggctgaatac gtctttgcct ctacgcagga gttgcagagc
     3001 gaaagcaaca gcggaaaatc tgtcaaaaca gtatcgttca aaacatattt tttgaagacg
     3061 gtaaagagga tgcggatggc ttttgatgtt tgccagccgt ccggagaact cggagacgaa
     3121 gaatccgctc tcgcgcaatg ttatatggca atcgccggct ttgttcgcaa aatgagcggc
     3181 acaaccgagg tagatacaga aacaatgaac cgccatgtat ccaaaatggt cgaagaggct
     3241 cttcagtaca acaaggttga aagtgtgctt agcgaaggcg aacaagaaga tattttcagc
     3301 cctgaatact ttgaaaaact gtccgatgtt aaattgccgg catnnnnnnn nnncaaatta
     3361 cagaataccg caaaacgaat caggtggaag caaagaagtt tcaggaaatg ctcgaaaaaa
     3421 cgatagaaga atatcacgag cgccgcaagc atttgacagt agaagaagcg ggcgaaacgc
     3481 aggagcaggc gtccgaagat attattaaat ctgcaaccga acaggcattg aagatattgc
     3541 gggagatgaa cgaaagccgt gaaagcttcc gcaaaatcgg attgactttt gaagaaaagg
     3601 cattttatga tatcttgatg tcattaagan nnnnnnnnnc agtataactt tgagtacggc
     3661 gaagataaaa ctgtcggcgg tatttccgta aatgataagt gcaaatcttt ggcaagaaaa
     3721 atcaaagaaa ttattgatac caaatcttca tttgccgatt ggcttaataa tcaaattgta
     3781 cgcgaccagc tgaaatttga cattaaggtt tgccttatca aaaatggtta tccgccgcag
     3841 tatagccccg aagtgtttca aaaagttatg gagcaggtcg aaaattttga ggaaaataat
     3901 tgatataaat aaaaattata tgccaacaat taaaaataac catggaaagt ttaaaatgag
     3961 tggggtaaca tatggaatca attttatttg acagttacag gaaataccaa aaagaacaaa
     4021 aagacaattt catacaggcg attttcaaat tgcctgtatg ggtacagatc tttttagcaa
     4081 tgattgttct ttgtttagtc tatagtgttt tggttctttt tgttccttgt cttagagtag
     4141 tgtacggtta ttgtttagcg gttgaagtat taacctgtgt ggcgttatat ttctatacag
     4201 aaaactttca aataaaaaca agtgattccc ggttgtttgt ttatcaagaa tactgtgaaa
     4261 aaatcaaaca ttggcttgat tcagttggcg tgaatgttac accagaaaat attacagaaa
     4321 taatgacgag aacaaataaa cgtattgatc tattagaaaa gaaacgcaaa gagcgaagag
     4381 atcgtattga tagatggat
//
LOCUS       DAWACS010000069         4388 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2172_length_4388_cov_1.680822, whole genome shotgun sequence.
ACCESSION   DAWACS010000069 DAWACS010000000
VERSION     DAWACS010000069.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2172_length_4388_cov_1.680822"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2340..2349
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2938..2947
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1488 a          708 c          976 g         1196 t
ORIGIN      
        1 atattgcatg gacaaccccc ggagttctta cttatacggc aaatgcgaga aacggtgcat
       61 ggcttgcact tgatgattac attgacacat atattccaaa gacaattgag cagcttggag
      121 aaatagccga caatgcaaga gtggacggca agctttatgc gataccgaca tataaggaaa
      181 tggcagattc aagaggctgg acatatcgca aagatattgc cgaaaaatac aatatcaata
      241 tggataatat taagacattt gacgaactgt taccggtgct taaaatgatt aaggaaaatg
      301 aaccaaatat gcaatatccg attgactggg gcagtgacag aacacctgaa gctcttataa
      361 agtacgagga aatcgcagga acagccgtta ttttctacga tacagacaaa tatgacggca
      421 aggttgtaaa tcttgtagaa actccggaat atcttgaagc atgcaaatgg gctaataagc
      481 tttataacga aggtcttgta aagaaagaca ttatgactgc tacggatttt gagcagagat
      541 taaaggacgg aaaaactttc tgttatgtgg acttcttaaa accaggtaag gccaaggaaa
      601 cttccgctaa gtttgatttt gaacttgacc aatcaacagt atcggatata tggcaggata
      661 atggagccgg aacaggttcg atgcttgctg tttcaagaac atcaaagaat ccggaaagag
      721 ttcttcgttt ccttgaacta cttaacactg atgcaactct cagcaacctt atcaactatg
      781 gtattgaagg aaaacattac accaagattg atgataatac tataacaatt ccggatgata
      841 catcgtatac gttacaaggt tatcagtgga tgcagggaaa tgtattccta aattatctta
      901 cagaaggaga aagccctgat aaggttgagg ctctgaaagc atttaacgca gaggctaaga
      961 aacctatcga ttatggcttt aaattcgata atactgcggt agaagcggaa atagcggcat
     1021 gtcagacagt taagagcgag tatcgtaaac aggtaattat gggttcaatg gaccctgagc
     1081 cgattatgaa agaatatgcc gctaaactta aggctgccgg cattgacaaa attattgaag
     1141 aagcacagaa acagtatgat gaatttcttg cgaacaagaa caagcaataa caagaacaaa
     1201 taattaggta taagtatagc ggtctttgac cgctatactt atatgaggga tatttaggag
     1261 atttagttat gaagaaatta gtttcattaa ttatcgcaat gtcaatattt ttttcaataa
     1321 actgtgctat ctttgcgaca aacgtgtcat atatggctga ttttgagtct gcagatgcga
     1381 aatttggtaa tagcacaaca tatagcggaa ctaaaaacac agccggcgat tatagtgatt
     1441 ttgtcaaacc agaatgggta gccgatggtg gaaaggaaaa ttcaacagga cttcgcataa
     1501 cctacaaagc tgcgacatgg tatgcaggcg aggttttctt cccaattcct gtagcttggc
     1561 aaaacggtgc ggatgctgag taccttaatt ttgattacaa tggcaaaggt attgtaaata
     1621 tcagcctgtc aacaggaagt gccgcaacag acactcttac gaaaggtaca aagtacagtt
     1681 ataaacttaa tgccgataca aacggtgaat ggcaaagtat aagtattcca ctttcggaat
     1741 ttaaaaataa cggaaatcct gtaacaatag ccaatatagg ctgcgtgaca ttccaagcgg
     1801 gcgaaaacgg cggtttgagt aacagtgcct ctgaaacgaa agcaatgacg gcagcggagc
     1861 ttgaagcgaa agcgagaaac ggaagtataa tctttgacaa tatggagttg tccaatgtcg
     1921 gagagaatgt tcttaatcca aatgcaacac cggaacctac agagaaaccg gataatacaa
     1981 caagaaccat agattttgat acttatactc tcagtcataa gcagacctgg gcaggattta
     2041 ataacaatga taagacctac agtgacagta taaagtcaga aataactgaa aacggtaaag
     2101 agggttgtgc tcttgaactg acttacaaag ctgccacatg gtatgccgga gagatattta
     2161 tgtcaatacc aaaggaatgg gcaataaata aaaattcaga gtgtcttgaa tttgatgcta
     2221 agggacaagg aaaaataaaa atatctcttg aaacgggcga ggttgtaaac ggaatacgat
     2281 atggtcatac agtgactata aacacaaatg atgaatggca aaaaatcagt gtgccacttn
     2341 nnnnnnnnng tgccactttc tgaatttgta aataacggta atgaagtgcc gttgacagat
     2401 gtggtaggaa tggctttttc tgcggcagaa agtggaaacc tcgataacaa tgctgaagaa
     2461 acaaagatga tgagtgcaga tgaacttgaa gaaaaagctg taacaggatg tgtagtaata
     2521 gataacatca cgcttgcgga acaggataca acttccccga cagctgctcc ggaagcgaca
     2581 actcaaccga cagaaatatc gtatgttgcg gattttgaaa cagcggatac taagttcgca
     2641 agcggtaaga catggggagg attcaaaaat aagtcgaatg attatcaaga ctatataaaa
     2701 gcaaaatggc tgcaggacgg cggagttgat ggaagtacgg ctttctgtgt ttactatcaa
     2761 tcggcaactt attatgccgg cgaaattttt gtgccggctc ctgcagtgtg gacaaataat
     2821 ggtgcaaagg gggctgagta cctaaacttt gattataagg gtaaaggagc tgtaaaaata
     2881 agtttttcaa cgggcaacac tgtcgatgga acgcttacaa gtggtacacg ttatacgnnn
     2941 nnnnnnnaag gcaatgagtg cagatgaact caaagagata gcgagaactg gtgagattat
     3001 ctttgataat atgacactgt cggaaacaga aggaaaaaca actctgtttt catctgttaa
     3061 agtaacagcg gaaattgacg gaaaggaaat cactaatctt acaaatggag atataaaaat
     3121 aaaagcgata gcatctgata ttgaaaaaga tacaaatatg gtaatgattg tagctgtgta
     3181 taaagaaaac ggtgtgattg atacagttcg tatggctggg cagaaaataa tcggagacgg
     3241 tgaattgatg cttgacttga atgttacaga tgctgagcat caaactatga aagtgtttat
     3301 atttgatgat tttacaaatc ttcacccgat aatcaatgtg acaaactttt tataaattaa
     3361 accacctagc ttaaagttta cctcatttta atgaaaaata gtacatgtta taataattgc
     3421 aagataaaga gatttatata tctctttatc ttgcaattat taagttttta aggagtggat
     3481 gagacgtgaa aaaaaagaaa agccttataa gagatttaaa agaaaatggt tccttgctct
     3541 caatgtgcat tccaggatta atatttttta tactgtttaa ttatttgcca atgtttggta
     3601 ttataattgc gtttaagcag tatcgttatg atcttggaat atgggcaagc ccgtggaatg
     3661 gactcaaaaa ttttgagttc atgtttagca gtccggacgc atgggttatt acgagaaata
     3721 cgatagcata taatttgttg tttattttcg gcggtcttgt atttaatgtg gcaatggcaa
     3781 tcggactttc tgaattgagg aataaagcag tttcaaagct gtgccagaca gtagtaatta
     3841 tgccgcattt cttatcatac gtaattgtaa gctttcttgt gctggcattt cttcacgttg
     3901 agaacggtct gataaataga agtcttattc cggcacttgg attagaaggt gtagactggt
     3961 actcaaatcc gaaatactgg ccatggattc ttgttatagt aaacttttgg aaaacaacgg
     4021 gatatggcag tgttgtatat cttgcaggta tagcaggaat tgacacctcg ctttatgaag
     4081 ccgctaaggt tgacggagca tctcgatggc agcaaatacg atacataaca ttgccggcac
     4141 ttgttcctct tatggtagtt cttacaattt taaatgtcgg aaagatattc aattccgatt
     4201 ttggtttatt ctatcaagtg ccgttaaata ccggtgccct ttatccggct acaaatgtta
     4261 tcagtacata cgtatataat atgttgatgt cagcgggtac gggaagtgta ggaatggctt
     4321 cagcggcagc tttttatcaa tcaattgttg gatttattct ggtgttttta actaatctgc
     4381 tggttcgg
//
LOCUS       DAWACS010000070         4341 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2201_length_4341_cov_2.502100, whole genome shotgun sequence.
ACCESSION   DAWACS010000070 DAWACS010000000
VERSION     DAWACS010000070.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2201_length_4341_cov_2.502100"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1710..1719
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2356..2365
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1273 a          859 c          865 g         1324 t
ORIGIN      
        1 atgttcgacc gaaagccgat aaaaaaagta tcggtgcaaa agatatgcgt aaacttgagg
       61 aggatgtggc tgtaaagccg tttaattcaa agcataaggt ttatatattt gaggacgcat
      121 ctttgcttac cgaggaggca caaaacacgt ttttaaaaac gtttgaagaa ccgccggaat
      181 acgcagtatt catcttgata acagaaaact ccgcttcact attgcagaca attttatcac
      241 gttttacgct tgtgcatttt ccgtctgtgt ctgacgtgat tatggagaag tatataagtg
      301 aaaaatatcc cgaagaaaag gaacgcttgc cgtttttgat taagtattgt gcaggtgtgc
      361 cgggagtggc tgataatatt ataaatgatg aaaactttga aacagtgaga acatcctcac
      421 ttgaaaattt attttcggta ttgtcaacag acagaagttc ggcattcgtt gtgaagaagt
      481 atcttgatga aaataaagat aaggctgata atatattcgt tttttggctt gcatatctgc
      541 gtgatataat tcttatgcaa tcgcaagcac cgaatatgat tattaatatt gataaaaaag
      601 atacattaag acagcttgcg gctaagtatg ataccgatta tttgatgaat atgtcaaagc
      661 gtgtaataaa agcaaaaaat atgttggcac gatatgtgag tttaaaagcc atttcgctgt
      721 ggttgtcgct aaaatccgca taaaacgcgg tatttctata aaaattattt ttgttagtat
      781 tttttctgtt tttgttgaca atttattaca tatgtggtat aataaattta ccgtttaaaa
      841 gaatattaga taaggaggaa aattttaatg aaatctgtag gaatcgttag aaaggttgat
      901 gaacttggcc gtattgtcat tccggtagaa ctaaggagaa agtttggaat cgacattaag
      961 gattcaatgg aaatttatac agaagataac tgtataatcc taaagaagta tgaaccttca
     1021 tgcatattct gtggcgatgc aaagaatata ttccaattca acggtaagaa tatttgccct
     1081 gagtgtgcta aaaagattaa cggctatgta gctgaataat catatcaaaa ataagaaccg
     1141 cttagtctga tgcggttctt tttttgtgtt gataataact ttaaattgtg gataaagtat
     1201 tttttagtac ggtttaatgt tgataaaacg ttaaaataca acattccata ccaacataca
     1261 aaaggctccc cttgagggga gctgtcgctt cgcgactgag tggtggctct tataaaatat
     1321 ataaagctgt cgaccaatgg tcgcctctac aaatgttatc tcaatacata aggctcccct
     1381 tgaggggagc tgtcgcatta cgacagaggg gtggctttat ataagatata caaagctggt
     1441 gaccaatggt cgcccctacg cacacattac ctcaatacac aagggagctg tcgcattgcg
     1501 actgaggggt ggctcttata taatctcttg ttttaaaatc ctatgagtct gtaaagcaga
     1561 tactctcccc tatcttgaca tcaataataa aagaacacaa aaaaagatgt taccgaagta
     1621 acatcttttt gaataatcaa atcagtaaag cttacgcttc ttcgatttca gaaacgacac
     1681 ctgaacctac tgttctacca ccttcacggn nnnnnnnnnc gattggagtg ctaacttcaa
     1741 cttccatctt tacgttatcg ccaggcatac acatttctgt gccttctgga agtgtgataa
     1801 cgccagtaac gtcagttgtt ctgaaataga actgaggtct gtagttgttg aagaatggtg
     1861 tatgacggcc accttcgtcc tttgttagta cgtaaacttc gcccttaaac tttgtgtgtg
     1921 ggtgaattgt accaggctta gaaagaacct gacctctttc aatttcgttt ctctgaacac
     1981 cacgaagtag tgcaccgatg ttgtcgccgg cttcagcgta gtcaagaagc tttctgaaca
     2041 tttcgatacc tgtaacaaca acttttctct tttcgtctgt aagaccaacg atttcaactt
     2101 cgtcgttaag gtttagctga cctctttcaa ctctacctgt agcaactgta ccacgacctg
     2161 tgattgagaa aatgtcttcg ataggcatta ggaatggaag gtctgacttt ctttcaggag
     2221 ttgggatata tctgtcaact tcgtccataa ggtcaagaat acacttgtat tcaggagcgt
     2281 taggatctgt tgaatcacat tcaagtacct gaagagctga acctgaaatg attggagtat
     2341 cgtcgccagg gaagtnnnnn nnnnnctgat ttccatttca acaagttcca taagttccgg
     2401 atcgtcaacc tggtcagcct tgttcatgaa tacaacgata taaggaacgc caacctgtct
     2461 tgctagaagg atgtgttctc ttgtctgagg cattggaccg tcagcagctg aaacaacaag
     2521 gatagcgccg tccatctgag cagcacctgt gatcatgttc tttacgtagt cagcgtgtcc
     2581 cgggcagtca acgtgagcat agtgacgtgt gtctgtttcg tactcaacgt gagctgttga
     2641 gatagtgata cctctttctc tttcttccgg agccttatcg atcatgtcgt aagcttcgaa
     2701 ttgagcctga cccttaagag ccaatacctt tgtgatagca gctgttagag ttgtcttacc
     2761 atggtcaacg tgaccgatag taccaatgtt aacatgcggc ttagttcttt caaatttctc
     2821 ttttgccatt gtagaaattc ctccttaaaa tattttatat acattaataa acttattgta
     2881 caatcttttg gtgaaaaaat caagtatttt ttcaataaat ttataaatta ttaaaattta
     2941 aacggcgaaa gtaggagaag atcctactta ccgtataaaa ttcaattagt tctttgcacg
     3001 ttcgttcata atcttttctt gaacagactt cggaacttcg ctgtagtgtg aaggctccat
     3061 tgagtactgt ccacgtccct gtgtacctga acgaagaact gtagcataac caaacatttc
     3121 tgcaagtggc acgtcagctg ttacctgagt aacaccgccg ctgcgtgatt cttgctgttt
     3181 aaccatacca cgtcttgatg aaaggtcacc gataacgaaa ccaaggtatt cgtcaggaac
     3241 gataacatta acaagcatga taggctcctt aataacagga tcagccttct tcataccttc
     3301 cttgaacgcc atagaagcgg caatcttaaa tgccatttct gatgagtcga cttcatgata
     3361 tgaaccgtca tatagagttg ccttaacgtc aactacgttg taacctgcaa gtacaccaga
     3421 ctgcatagca ccttggatac ctgcgtcaac agcagggata tattccttag gaatagcacc
     3481 gccgacgatt gcattaacga attcataacc agcaccttct tcaagcggct caagtttaag
     3541 aagtacgtga ccgtactgac ctttaccacc tgattgacga gcatatttat gttcgatatt
     3601 aactgctttt ctgatagcct ctctgtaaga aacctgaggt tcaccaacgt tagcctcaac
     3661 cttaaactca cgaagaagtc tgtcaacgat aatttcaagg tgaagttcac ccatacctgc
     3721 gatgattgtt tgacctgttt cctcatctgt gtaagtcttg aatgttggat cttcttctgc
     3781 aagttttgca agagcgatac ccatcttttc ttgacctgcc ttagtcttag gctcgatagc
     3841 aacacggata accggttcag gcaagtccat agactcaagg ataattgggt gcttttcatc
     3901 acaaagtgtt tcacctgttg ttgtattttg tagaccaaca gctgcagcta tatcacctga
     3961 gtaaactata tcaaggtctt ctctgtggtt agcgtgcatt tgtagaattc tacccattct
     4021 ttctctctta cccttgcatg agttcaatac atatgaacct ttttcaagag tacctgagta
     4081 tactctgaag aaacaaatct taccaacgaa tgggtcagtt gcaatcttga atgcaagagc
     4141 agcgaacgga gcgtcgtctg atgacggtct ttcgtcctct tcgtctgtat caggatttac
     4201 acccttgata gcaggtacat ctaccggtga aggcatgtac tcaataactg cgtcaagaag
     4261 catttgaaca cccttgtttc tgtaagctgt tccgcaaagc ataggaacga tttcattatt
     4321 gatagtagct tttctcaaag c
//
LOCUS       DAWACS010000071         4306 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2217_length_4306_cov_1.871795, whole genome shotgun sequence.
ACCESSION   DAWACS010000071 DAWACS010000000
VERSION     DAWACS010000071.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2217_length_4306_cov_1.871795"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2250..2259
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3245..3254
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4033..4062
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1364 a          843 c          745 g         1304 t
ORIGIN      
        1 cttttaagta agcaccgacc ttacgcagta caaccgcatt tgtatcggca tttttcataa
       61 taccgagtat aatttcaaca ctcacaaaac ttgatttagt cgacccacga ccaccgcaaa
      121 gccaaaaatg cgtataatct tcaatagagt tatgcacctt gtagaaagac ggcgctatca
      181 gcctgtcaag actgcactgc atttttccgc tccttgattt gttcggcttt ttcgatatta
      241 tcgacgatta taacatcacc gcctccgctt tcgctcttgc ctccgaacat tccgtaatgc
      301 ttacccaaaa gctccgccgc tttaaatgaa tccgccatac gaatttcctc atcttcgcga
      361 cgcattatgt acgttagaaa ttcaagaatt tccccttcat ttgcaatgca caatttaatc
      421 accctctttt tcatttaata ttgatttttc atcaatacat aacgtccgac tttctgacac
      481 attcactgca gccgacacat tcttcatcat cattgatata gtaatgttcc ggctcaaaag
      541 caccgcatac gggacatacc gttcccccgt catatgagtc atactcaccg taattatccg
      601 tccttgcaat ttccattgcc aaaattgcca ttgggtcatc atttgaataa atcataagca
      661 ctcctttcta tcttttcaag atactttatt gttaaaaaat atatgccaat cacttggcta
      721 tctctttaag acactgttat tatatctcat caagatactt ttgtcaagta taaatttctt
      781 tttaggatac ttttaacaac attttgttta ctttttagat acttttatgt acaatgcttg
      841 caaaaagttt caaaatatga tactatataa tcagttacga aatatgatta gaaagatttt
      901 ggtgattaaa atgactacgg gcgaaagaat taaaatgctc agaaaagagc ataatttaac
      961 ccaggaggag cttggagcaa aaatcggtgt gcaaaaagcg gcaatccaaa aatacgaaaa
     1021 agggacagta aaaaacataa aacgcgactc gcttataaag cttgcacaat gtcttgatac
     1081 ttcccccgaa tatcttctcg gctgggagga tatgccgaat aacgttgaaa tagccgatac
     1141 gtcaaactat gtaaatattc cgataatcgg cagagttgcg gcaggtctga gctgctttgc
     1201 cgaaacgaat atcacagatt acgaacctgt caataaaaat gacgttcgcg gtgacgaacc
     1261 gttcgttttt ctccgtgttg tcggtgacag tatgtatccg ctttttatgg agggcgacct
     1321 tgtgcttgtc agatgtcagt catcggttga cagtggcagt tatgcggttg ttatgataga
     1381 cgaagaggac ggcgttgtaa agaaaatcgt atacggacct gattttatcg aacttcactc
     1441 cataaaccca atgtaccctg tcagacgttt tgaaaacgaa gatgttacac gtattcgtgt
     1501 attcggcttg gtacgcgaga taaagcgaaa attttagtcg aatttggaac aaatcagtat
     1561 aaatttacgt ctaataatat atatgataaa ggagtataat attgtatcaa tataaaacac
     1621 tttcaaacgg tataaaagta gttgccgaaa agattgatta cctaaagtca atctcaatcg
     1681 gagtatgggt aggcaacggc tcacgatatg aaaataaaga agtaaacgga atttctcatt
     1741 ttatcgagca tatgcttttt aagggaacga aaaatcgctc tgcggcacaa attgcacacg
     1801 aaatagattc ggtcggcggt caacttgacg catttacttc gcgtgaatat acttgttttt
     1861 atacaaaaac acttgattcg catattccga ttgcacttga aattctttcg gatatgcttt
     1921 ataatccgag tctttcacct gatgatatgg cacttgaaag acaggttata aaagaagaaa
     1981 tccatatgta tgaggacagt ccggaggatc ttgtgtatga cctttcgtca tatgccgcat
     2041 ggggcgacac acctatgggc agaacgattc tcggcacata tgaaagcctt gacggcataa
     2101 caccggatat aatgagaaac tatatgaata atcattatac aagtgcgaat actataatat
     2161 cagtatcagg aaattttgac gatacgtttt ttgaacttct tgaaaagtat ttcggtacaa
     2221 aaaaaatgct tgacacaaaa ccgtatcttn nnnnnnnnnt cccgatgcac cttatataag
     2281 caagaataat atcgtaaaag aaaaagatat tgaacaagtc cagcttgtcg caacgttcaa
     2341 aggcattgat gttatggacg aatcggtgta cagtctgctt gtgtttaaca acgtgttcgg
     2401 cagcggaatg tcgtcaagat tgttccaaaa catacgcgaa cagcgtggtt tggtgtattc
     2461 gataagtgcg ggacacagtg cgtatatcaa tacgggtgta tttgatattt ctgccggaat
     2521 gagtcccgaa agtcttggcg aagtcgcaca tcttatttca aaagaaatca atacgattaa
     2581 acacaataag ctgacatcgg cagaaatcac aaaagcaaaa gaacagctta aaggtaatta
     2641 tatactttca agcgaaagca caggcgcaag aatgcaaggt gcaggtcgtt cattgctttt
     2701 gaataaaccg atatacacgc aggaagaagc actgaaaaaa atcgacgcag taaatgccga
     2761 ttcggttgcg gaaataatag acagagttct cgacagttcg actatgtgta tttcagcagt
     2821 cggacctgtc aataacgttg acaatctgtt tgaactcgat taaataatgt ataaatatta
     2881 atttattata aaattttagc taaaacactt gcaattttat tgtatttgta ctatgctata
     2941 aataaatgat atgctatttt tagcagaaag ggtgaatatc atggaattta acgaaacaat
     3001 gaaaatcaac cttgagtttg ataaggaaaa ggaagtcaga gaaatagttg ccaaagtata
     3061 tgaggcactg aaaatcaaag gctatgatcc tatcagccaa atagtaggat atatcctttc
     3121 aggcgacccg acctacataa caagctataa cggtgcaaga ggtctgattg ttaaaataga
     3181 acgtgacgaa cttttagagg aatttgcaaa aacatatatt aaaacgcttt aatcaaataa
     3241 aaggnnnnnn nnnngtattt tagtcatacc ttttgtttgt acgcgaaaaa tccgtcattt
     3301 gacggatttt tttagtttca tcacaatatt ttctttaata cctgcattac accctcatca
     3361 tcatttgacg gtgcgatgta ttttgcaact tttttaattt catcgtgtgc attttccatg
     3421 gcgtaacttt cgccgcaatt ttggagcatt tcataatcat tcatataatc accgaacgca
     3481 acacattctt cgggcgaaat accgtaaatt tcctgaataa ccttaattgc gttgccctta
     3541 tttacatttg cattaataac atcaacccaa ctttccgccg ataaaacagc cttcaaatca
     3601 ccgcttatat ccgcgagttt gggaagtata ttatcctttg ctcttttgtg gcagtaaagc
     3661 gcaagattta aaatattatc cttgcccacc accgatttta tgtcatcaac agtttccaaa
     3721 cgcacattat attgtgcgac atcgttcata acatattcag gcgggttttc tatataagat
     3781 gtcttttcgc acccaagcac aggataaatc cctttgtcct cactcaccaa atcaagcact
     3841 tccaatgcct tatcgtcagg aacgggcata atatgaataa ccttgtcctt ttccattata
     3901 attccgccgt tttcagaaat aaaaatcaat ttatccttaa tcggattaaa tatattcaaa
     3961 agactgtaat actgcctgcc acttgcgaca acaaatttca cattgggatg tgattcaatc
     4021 atttcaaaca gannnnnnnn nnnnnnnnnn nnnnnnnnnn nntttactgt ccaaaacagt
     4081 tccgtccata tcaaatgcaa caagttttat tgatttttcc acactaaaaa acctcctttt
     4141 gcttattata taccgcaaag gaggtaatgt aaacatttat tttttgtcag tcttttttat
     4201 tttaattatg tattccgttc cccattcaaa agaattttct tcgattcttg cgtctatgcc
     4261 gctttttttc atcaaatcaa ccgcacgttt tacggtgttt ttaaat
//
LOCUS       DAWACS010000072         4304 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2218_length_4304_cov_2.558484, whole genome shotgun sequence.
ACCESSION   DAWACS010000072 DAWACS010000000
VERSION     DAWACS010000072.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2218_length_4304_cov_2.558484"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3170..3179
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1266 a          786 c          978 g         1264 t
ORIGIN      
        1 aacaaagtag acaaaaccat aacggttttg tctacaatct gaaatgcctt tctctttgag
       61 aaaggcattt tggtttatag tccaagttgt tttttgcaat cttcaagaac tttttcgatg
      121 gtcttttcgg ctgtttcaaa gctttctgcg aatgtgccga gatacaactt gattttcggc
      181 tctgttcccg acggtcttac gataaagtaa gtcttttcgt ctgaaaggtt gtacttcaat
      241 acgtttgact tcggcaaacc tttaagaggt gaaacattgc cctttgtatc tttaattgtt
      301 tgtgactgat agtcggtgta aagtacaacg tccatacctg caacttttgt cggcggtgtt
      361 tgacgtaggt ctgtaagtaa ctgtgacatc ttttccatac cgtctttacc cggcattaca
      421 aatgaaactg tcttttctga ataatatccg tacttcttgt atatatccat aagtgcttcg
      481 taaagtgact taccctttgt tttgtagtaa gcagccattt ctgcgataag cattgtcgca
      541 acaacgccgt ctttgtcgcg tgcgtgagta cctacaagat aaccgtagct ttcttcaaag
      601 ccgataagat atgtatggtc gcctgtcttt gcgaactctg tcattttttc gcctctgtac
      661 ttaaagcctg taagcacgtt cataatttcg ataccgtatg aatgagcgat tgcggcggca
      721 agctctgttg taacgattgt cttgataaca acgccgtttt caggcagagt gcctttggct
      781 ttctttgatg aaagtatgta ttcaacaagc aaagcacctg tttggttgcc tgtaagtgtt
      841 ctgtatatgc cgtcggcgtc acgaacaact ataccgactc tgtcacagtc ggggtctgta
      901 ccgatgataa cgtcaacgtc attttccttt gccattttta tagcgatttc aaaaccttcc
      961 ttgtcctccg gattaggtga cttaacagtt gggaaattac cgtcctctgt atcctgttcc
     1021 ttaacgacaa gcacattttt aaagcccata cggttaagta ttgctttgat aggtcttgag
     1081 cctgtgccgt ggaaaggtgt gtagataagc ttgaatgtat ctgcaacttc tgctactgcc
     1141 tcgggatttt gctgctgtgt ttgaacaaca tcaaggaatt tttcgtcaag gtcactgctc
     1201 acgatttcaa gcaagccttt ttcttttgcc tcgtcaagcg aaataaattt aacatcgtca
     1261 aatatatcgt agctgtcgat tgcggcaaca acaacgtcag ccgcgtccgg aggaagttgt
     1321 ccgccgtcag ggccgtatac cttgtaaccg ttgtattctt tagggttgtg gctggcggta
     1381 atcataacgc cggcactgca gccaagttcg cgaataccga atgaaacttc cggaactgaa
     1441 tgaacgatag ggaaaagata agtctttaca cctgcacaag taagaacctt agctgtttct
     1501 tcagcaaatg tgcgtgaaaa attacgtgta tcgtaaccga taagaacacc tgctttggcg
     1561 gcgtcctcac cgtttgaaag aacgtatttc gcaacacctt gacttgcacg tcttacgtta
     1621 tatatattga ttctgttcgt acccgctccg ataatacctc tcataccggc tgtaccgaat
     1681 tcaagttcac gataaaatct ttcttctatt tctttttcgt tgttttcaat agatttaagc
     1741 tctgcttttg tgtcatcatc aaccacatcg cttgtaagcc aacggttgta ggtttccttg
     1801 tatcccattg ataactcctc cttaaaaata atctcatatt aatattatat aatactttct
     1861 caaaattttc aattatgaat atgtaaaaat taaagaaaag ttatcgtata aattgacaaa
     1921 aaaaacgcaa aacttgacga aaagtgtatt ttgttataga atagcagtaa tatgtattaa
     1981 taaggaggac acttttatgg cagtaaaggt gcatgaaaca ggttacggca aatggaaagt
     2041 gcctgtgata atcatagctg ttatagttgt tttgggtctt gcgggaacgg gcatactatt
     2101 aagcgacagc cttggtgttt caatagaaga aggaagtaag agaatagagg ttgcagaagg
     2161 tgacggtacg tcatctgttg caaaactgtt aaaggacaac ggcataataa agtatccgct
     2221 tttgtttaag gtgcagtcaa agctcggcgg atatgacggt aattttcagc cgggtgcggc
     2281 aactattgat gacggaatga gttacagtga catacttaat cttataataa cgccgagccg
     2341 tgaggctgcg aaaattacaa taccggaggg ctatgaaatc aagcagattg cacagaaact
     2401 tgatgaagca ggtattgtaa gctggcagga cttttatgcg gctttaaatc ccgacgatta
     2461 tgattatgcg ttcttgaaaa acttgcctga acgtgacgga cgtatggagg gatatttgtt
     2521 ccctgcaacg tatgaaattc cgaacggaat gtcggcacac gatattatcg acctaatgct
     2581 tgcggcattt gacaatcagt ttaaaccgga gtattatgaa cgtgcgacag agcttggatt
     2641 gacggttgat gaagttataa ctatggcgtc aatcgttgaa cgtgaaacgg acagcggaac
     2701 ggaaagagca aaagttgcgg gagtatttta taatagaaga aactcaggta tgaagttcca
     2761 gtcgtgtgcg acggtacagt atattttagg tgaaagaaag cctgtacttt cgattgccga
     2821 cacgcagata gattcaccgt ataacacata tatgtatgcg ggatttccga taggtcctat
     2881 atgcaatccg ggaacggaat gtatagaggc ggcactttat ccggaggcaa ccgatgcgta
     2941 ttactttgta cttggcaagg acggctcaca tatcttctca aagacatatg aagaacacct
     3001 tgcggcaatg cagagtaccg aacaaaatat ttcggtcgat gacagtgcac ttgaaaatca
     3061 agacagttta aaacaataaa gaggtttgat aaatgataga atatgataaa gacgcgattg
     3121 ttccgccgta tataacggaa tatttgcgtg cgaaaacagt gcataatgan nnnnnnnnna
     3181 tgcactcgct tgaaaaatat gcggaagaaa acagtgttcc gatagtagag cctgaaacgg
     3241 caaggttttt gtcggtgatg tgcagaacgg ttaaaccgaa aaaaatactt gaagtaggtt
     3301 gtgcgattgg gtattcttct atattaatgg cacaggctac agatgatgac tgcgaaatag
     3361 caacacttga atgtaacgaa gatatggtgc gtattgcaag ggagaatatt aaaacttcgg
     3421 gatatgcgga aaaaatatct gttattgagg cagatgcaaa ggattatctt tcgtatattg
     3481 acgatgatga agtttttgat atgatattcc ttgacggtcc gaaagcacat tatatttata
     3541 tgcttgacga gtgcgtgcga ttgctgaaaa aaggcggtgt gcttatttcc gacaatgtgc
     3601 tttacaaggg aatgacagcg gacgataacc acgttgtgag aagaaaaatc acgactgtaa
     3661 aacgtcttag aaaatatatt gatatgctta tggagcataa ggagcttgaa acatcgcttt
     3721 taccgctcgg tgacggtgtt acaatatcgg taaagaaata ataaagggta ggaatatatt
     3781 atgaagaaac cagaactttt agctcccggc ggcagcttgg agaaattgaa aacagcgata
     3841 gattacggtg cggacgcggt gtatatcggc ggtgaaatgt tttcgctgcg tgtagcggcg
     3901 gaaaattttt caaaggaaga tatgcaggag ggtataaagt acgctcacga cagaggcaaa
     3961 aaagtgtacc ttaccgcaaa tattcttccg cataatgatg atattgaaga atttgaggag
     4021 tttgtcaaag atataaagaa ttacggcttt gacgcagtgc ttgtggcaga tttgggattg
     4081 tttgatatga tgcaggaact tgcaccggaa atacctattc atgtaagtac acaggcgaat
     4141 aacattaatt atcgctcggc aataaagtgg tacaagatgg gtgcaacgcg tgtcgtgctt
     4201 gcaagagaaa tgtcgtttaa agaaattgcg gaatttcaaa agaaaattcc tgaagatttg
     4261 gagcttgaag catttataca cggcgctatg tgtatatcgt attc
//
LOCUS       DAWACS010000073         4294 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2224_length_4294_cov_2.597782, whole genome shotgun sequence.
ACCESSION   DAWACS010000073 DAWACS010000000
VERSION     DAWACS010000073.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2224_length_4294_cov_2.597782"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1006 a         1010 c          812 g         1466 t
ORIGIN      
        1 gataaatact tatagcgcgg gtgcgccgtt atatcgtact tttcagacaa aaacggacgg
       61 acgcccctta attgcaaaat gcacttgccg ccgtccatta ccgataattc gtcctgcgtc
      121 atcagctcct ttcctaattt ttgataatta agtccgtaag atttttgatt acttcgcgtt
      181 tcggaagtat tgtacatatc aatcgtttct ttgccgagcg tttcggaaat ctctttaaga
      241 gtagttttct ctttacctcc gagaaatagg gtagtatcgc aattacctat aatcgtgtcg
      301 gcgttatcct tgtaaatagc ttttagctga ctttgcgcct gcaagattat tgacgccgat
      361 atttctcggc ttcttattgt ggcaatcagc ttttcaaatt ttggtatttg tccgatattc
      421 gccatttcat caagcagaaa tctaacgtgt acgggaagtc tgccgccgta aacgtcgtcc
      481 gccttatcac aaagcagatt gaataactgc gtgtacatca tagcgactat gaaattaaaa
      541 gtatcgtccg tatcggaaat gataacgaac aacgccgttt tcctgtcgcc gattttgtcg
      601 agctccaatt cgtcatattc cattaagtcc ctgagttcct taatgtcaaa cggcgctaat
      661 ctcgcgccgc acgaaatcaa aatactttta gcagtcttgc cggctgccat tttgtatttc
      721 ttatactgct ttactgcgaa atgctccggt tccttttctt ccagacggtc aaagagtaaa
      781 tcaacggggc ttttataatt ctcgtcgtcc tcttttgctt ccgacgcgtt tatcatatcc
      841 aaaagagtta tgatgttctt ttcctcgtcg ggcgcttcgt accaaatata gcctatgagg
      901 gcgcaataat acaggcgttc agccttaacc cagaaatcct cacttgactt ttcgccctcg
      961 cctttggtat tcgcaataag ggtattaacg agcttcaaaa tatccttttc gctgcgtatg
     1021 tactcaaacg gattatattt catactcttt ttgaaattta tcgtattaaa tgtttttatg
     1081 atatatccgc ctttttgtaa cagcttcccg cactcaatta aaaccgtacc tttcggatcg
     1141 gttacaacat agctgctgtg catttgcatt aaattcggtt ttatgaaaaa ccttgtctta
     1201 ccgctgccgc tgccgccgat tacaagaaca ttcttatttc ttgcgtactt cggcagcttc
     1261 gggcggctgt ccattgtaag ccgttccgta ttcgtgagaa gaacattatt atagaaattc
     1321 tgtccgtcaa tatacggggc tatgtctgac gccgtacccc agcgcgccga accgtattca
     1381 atgccgtttc ggtatttctt agcattttta cccctgacat aaactgcgca cttaatcaga
     1441 agcgcggcta ttactccgat tgatatatcg attatgtgga acgaaaaaag cggagctttg
     1501 taaaactccg caattccctg aaccaacgct attattttat taccctctgt tatcctgtat
     1561 atatagcata tcttatcagc aatccaaaag aacagaagat acgggatatt taaaagaata
     1621 aaccttttgt ctatcctcat atatcgcgct cctgttcttt gcggcgggcg cggtctttcg
     1681 gattgatagt ctttgcaagc tcgcgcattt gagataacat tttacggatt gacggttttt
     1741 cccgtaacag cttttgcgaa tactcctgaa acgccatatt tatgacatca atatccttac
     1801 ccttgaagaa tacgaggtac ttcggcggtt tcgtggaatt atcctttttc aaagcatagt
     1861 caagtccgta ctttttcgcc accggctcaa aagattttat atttttattc gttatctcaa
     1921 tatttgaaag cgccgcgcct gactctacaa gctgcttaat cgtctgttta ccctgatagc
     1981 ccttgttttt atttttctgc tgctgtctta ggtattcacg aattgccgcc tgcaatacgc
     2041 gccccgtcat tttggaagcg cttataacta acgcaactgt ttttttattt atttcttcct
     2101 gtatagacat cactccttgc aacaaaaaag cggctgttta ttttcaaaca accgcttatt
     2161 gcttaatatt ctctgtatct gatttatgga ttatttcttc cgccttatcc aaaccgttta
     2221 taatccactc gtcaaattca gtctgtaccg tatttgtcgg ttctatgcgt tcctccaccg
     2281 tttacacccc ttatgtataa tccaattata ataccaatgc ttttttagga cagaaatttg
     2341 aacatttacc gcaccctata cacttgtcat aatctattat cggtgcttca aaatctttct
     2401 gacttaacgc tccaacagga caaacattga cagcggggca tttatgattt tggggacagt
     2461 tttctataat tatttttaat tttttatcca taatattctt tcctttcgat ataaaatatt
     2521 ataaagatta tatcatatcc ggtcaacaaa cacaatacac aaatcagaca attattttac
     2581 ccaagtttcg ccgctctttc cgtcaacggt attatgcgta ttgcttgctt caaggatttc
     2641 ataaaacgcc caatatgact tgtctttgag gtctgtaaat ttatttacgg ctgacatatt
     2701 cttattgatg tattccgtat cggcggcgcg tcctgttacg cggtttacaa tcgttacaac
     2761 ctccgcgcgt gtgatgttgc tgtccggctt aaatgtgctg tcgctgtatc cgtttatcca
     2821 gcccatacca acagcgctgt aaatataacc tgacgcccaa tgtgattttg gtacatcatt
     2881 aaaaatattc ttctttgccg ccactgtctt agcgtccagc gcatagaacc tcatacacat
     2941 tgttacaaat tcggctcttg taatatgttc gtccggctta aatgtcttat cgtcatagcc
     3001 ggatattacc ttgtactttt caagataggc gatatagtta ttaaaccaat ccttgcttga
     3061 tacgtcctta aatgatgatt tacggcttga aatattttcg cccttacgct ctgcaatatt
     3121 acgggcgaat atggcagctg cttctgctct tgtcatatta ttttcaggct taaaggctcc
     3181 gtcctcatat ccgactacat aagaattatg cgtgtttgtc ggcataataa gctgaccgtt
     3241 tgcgtcggtt gcgccgctga cggaattatt cttgtctttg agcgtaacat caatacccgc
     3301 cgttgtcttg cccttgttat ccgttacggt aattgtataa taattatcgt caagagtttt
     3361 gccgctcgga agcgtcaaag tgattttatc gtccttgacg gtcttattga cattgacggt
     3421 ctttccgtcc ttatcgacaa cctttacagt gtatgacggc ttgacatatc cgctactgcc
     3481 gccgcttcct cctgaagaag aaccgccgcc accgcctgtt gaagtcttga cgggtacagt
     3541 gattttaccg tttgcgtcgg ttgattttgt cgctgtctta ttatccttat cggtaacagt
     3601 tacggaaata tccttgaccg ctgtattatc cttgccgctg acggttacag tggtttgatt
     3661 atctgtattg agcttgtttc cgtcgggaag aataacgctg attttaccgt ccttaacctc
     3721 aacggcggca ttttcgattt ttgcctttgt gtcctcgaca atgacattgt atgtcttttc
     3781 tccctcaaca acctgtgcat agccgttttt gtctgtacgg tctgtacttg tcgggggaac
     3841 aatcgcaatg ccctcactgt ttgtaaggtc ttttgcttcc gttccgtcct ttgcaattac
     3901 ggtaacaggt acgccgttta cagcttttcc gtccttgtct gtaattccga taataattct
     3961 gttatcggga gtgataccct tttcatcagg gagcttaacc gtaacgctgc cgttgtcttt
     4021 atcaacactg acgattgcgc cgtttacagc gccgtcattg tcatttacag ataccttata
     4081 cgaataatcg ggagtaacaa cctcgcttcc attatcggga tttacggtag gcttcggctt
     4141 ttccgtttcg ctcggctgct ctgtcgcgtc cggcttttct gtttctgacg gtttatccgt
     4201 tgcgctcggt tcgtctgttg cgtccggctt ctctgacggt tcgggagttt caagtccggg
     4261 ttttgttgtc agagtaggct ccggcgtcgg ttta
//
LOCUS       DAWACS010000074         4288 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2233_length_4288_cov_2.353886, whole genome shotgun sequence.
ACCESSION   DAWACS010000074 DAWACS010000000
VERSION     DAWACS010000074.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2233_length_4288_cov_2.353886"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1293 a          951 c          723 g         1321 t
ORIGIN      
        1 gtccatatgt tcttcaagct ctgaaagtac ccaattatct atttgaaagc ctttttcttt
       61 tacaagttta tgataaatag gcggtttaat gtcagtaagc acaattctgt ggaaaaactc
      121 aaatattccg ccctcaataa gctttatcat attaacgctt tcgccctcac actttgcaag
      181 cacataagca taaaacatct tgtgtgcctg cttgtcaagc tccgtaaagc cggtccaagg
      241 acggatatgg tcgttccaac gctgaataga tgccgcctca tacatcagag aaataagaga
      301 ttttttaatc atttaatttt actccatcac aatattataa ccgttctttt caagtacctt
      361 aataactctt tcgccgtgtt ctctgccgcc gacttcacaa gcaatatgaa taattgcctc
      421 gttcggatca aggtctgcac tcaatctgtc gtgctgtacc attacgatat ttgcgtttgc
      481 ctcggcaaga atgtgtgaaa gatgttccaa acttcccgga atatcaagaa gtgtagtctt
      541 aaatttaagc tttctctcac gcgtaacaag accaagctcg ataattctgt gaataaagct
      601 aacatcaatg ttaccgccag aaagtacgca agcgattttc ttacccttaa cgtcaacctt
      661 gttgttaaga acagccgcaa gcggtgtagc accggcaggt tcaacaactt gctttgttct
      721 ttcaagaagc agtaaaatag cctctgagat ttccgcatct gataccgtta caacatcatc
      781 tgcatacttg ttgataagtt caacagtttt gtcacccggt attttaaccg caataccgtc
      841 tgcgatagta acggcagaat cggtagtaac gtgttttttc tctttaaatg aattgtaaat
      901 tgcaggagcg ccctcagctt gtacacctat aatttgtaca cgcggattga tttgctttat
      961 gcaagccgca acaccggcca aaagaccgcc gccgccagcc ggtacaagta ccatatcaac
     1021 agtaggtaaa tcctcaagaa tttctatacc gattgtacct tgtcctgcca taacttcaag
     1081 gtcgtcatat gggtgaagga aagttgcacc ctctttttct tgaatttcaa ccgctttttt
     1141 gtaagcgtcg tcataacaat ctccgtaaag cactaccttg gcaccgtaac cctctgtcgc
     1201 actcacctta gcgataggag cagacttcgg cataacgata gttgcgggaa tgttgtgaac
     1261 gtgtgacgca tatgccacac cttgtgcatg gttgccggca gatgaagcaa cggctgaagt
     1321 aatttcgcct ctttcaacaa gtgcggcaat cttatttgac gcacctctta ttttaaatga
     1381 acctgttttc tgttggttct caaattttaa atatatctct ccgcctgtca tgtcagagaa
     1441 agttttagat gattcaatct tagttctgtg aatagtcggc tcaagacgtt tagccgcaag
     1501 ttcaatttct tttaattcca aatccatttt tttcgcaagt cctttcttac ttttccttat
     1561 cttataatta taccgcaatt atatctattt ttcaatagca gattttattt atatgcaaaa
     1621 tcattatatt attcacaaaa caattcactg tctcccctcc tcaccatcct ttttcttcat
     1681 atgctttcca ttcgggcggt caatgaccgc ccctacacac tcccctcttt ttcccttcgt
     1741 ttcgtagggg cgaccaacgg tcgcccgtta tcccatatac tttccactcg ggcggccaac
     1801 ggtcaccctc ttaccataca caaaaatccc acaatcccct caccttccct tgcaaaccgc
     1861 cacaaaatat agtataataa aaaagttgca tatagagatc gaaaggagtc acaataaatg
     1921 gaacaaccga aagaaaataa aatgggtaca atgcctgtaa acaagctgct tgtaacaatg
     1981 tcactgccta tggttatatc aatgatagtg caagcacttt ataacatagt cgacagtata
     2041 ttcgtatcaa gactttccga agacgcattg acagccgttt caatggcatt tccaatgcaa
     2101 aaccttatga tttccgtagc agtaggaaca ggggtaggta taaacgcaat gctttcacgc
     2161 gcactcggcg aaaaaaagtt tgaagcggca aacaagaccg ccgaaaacgg tatatttata
     2221 gaagttctcg gatatgtttt atttttactt ataggaattt tcgtaacaaa gccgtttttt
     2281 cttgcacaag cgggtgcagg cgatattgca aatatgggta ttgaatatac aagaatttgt
     2341 ctgcttatgt cattcggtat atttatgcag attggttttg aaagaatcct tcaatccaca
     2401 ggacgaacaa tattcacaat gataacacag tcaacaggtg caattatcaa tatcatactc
     2461 gatcctatat taattttcgg tttgttcggt atgccgaaaa tgggtgttgc cggtgcggcg
     2521 attgcgactg taacaggtca aatatgtgcg gcaattcttg cgattacatt taaccttacc
     2581 aaaaatcccg atgtacatat cagctttaaa ggttttaaac cgcagattat atttgtaaag
     2641 aacattcttt ctgtcggtat tccttcaata attatgtcgt cagtcggctc tgcaatgact
     2701 ttcggtatga ataaaattct tatcactttt tcgtcaactg ccgttgcggt attcggtgta
     2761 tactttaaac ttaacagttt tgtatttatg cctgtattcg gtcttaataa cggtatggtg
     2821 ccgattgttt catataacta cggcgcacaa aacaaaaaac gtcttacaaa aaccattaag
     2881 ctcgcaatta tgtatgcggt ttgtattatg cttataggaa ttatgctgtt ccaatttatg
     2941 cccgatgtac ttttgaaatt gtttgatgca tcagaccata tgcttgaaat cggtattccg
     3001 gcattgagag taatcagcct cagctttgca tttgcaggta tatgtatagt tatttcatca
     3061 agcctgcaag cattgggaca cggcttttta agtatgatga tttctataac acgtcagctt
     3121 atcatacttc ttccgtcagc atatatcctt gcaaaattcg gcggtatcca cgcagtttgg
     3181 tggtcattca acattgccga aatcggctca ttgacgttat cattgttatt ctttaaacat
     3241 atgtataaca agataattaa acatttagga gaatagtaaa tatcccccgg ctataccggg
     3301 ggatatttat ttagcataga ttcttttatc acctctgatt agtattattt ctgctcaaat
     3361 ttggcaataa aaaaaacaaa ccgcataata atgcgatttg tttgccgttt ttggtcggga
     3421 tgacaggatt tgaacctgcg gcccctacgt cccgaacgta gtgctctacc aaactgagcc
     3481 acatcccgat atgacctata aacaacataa attattatac caccactctt ccgcaaagtc
     3541 aaccaccaaa acaaaattaa tccaatattt tttacaaagt aaaaagacgg attgctccgt
     3601 ctttttaaaa atcagtttca tcatcgtctg caaccgaaac aggttgttta acaatttgtt
     3661 cttctttaat ttttacttct tctttaactt catctatact cgatttcttc ttattaaata
     3721 tatgtgcaac ggtatcgtct acaaaataat gcgatataag cgcacacaac acagcaatag
     3781 cataaaccgc cgcaacatat cccatattac ccaccaaacc gatttgcacg aatatatcaa
     3841 caacaaccat atgatacaaa tacaatccgt acgacaaatc aaacttaaat ctgattttac
     3901 cgaaactgta accgaaaccg acagtcataa ggcaaagcaa aagcacttga atcatatact
     3961 cgcctattct cacaccgaac ctgtcgcaat aaatcgcacg ggcaataaac aaaatcacac
     4021 acaatatttt tgtttttgaa agtatcggca cgattttttc acgatagcgg tacataaacc
     4081 aacctgcaaa atacatataa aaatacggca tacacgaatg tcagatgatc aaccgtcccg
     4141 cttcgggcaa tatttcctgt aagtgtggca caacaagatt aagtgccata gacagtatca
     4201 ttacaaatat ccatacccac agttttcgat tttttaaaaa tctgtatatt atcgcagtaa
     4261 taatgtaaaa ctggactgta aatatcat
//
LOCUS       DAWACS010000075         4277 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2244_length_4277_cov_2.249171, whole genome shotgun sequence.
ACCESSION   DAWACS010000075 DAWACS010000000
VERSION     DAWACS010000075.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4277)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2244_length_4277_cov_2.249171"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    223..232
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1276 a          866 c          931 g         1194 t
ORIGIN      
        1 gtataataga atagaataat tttatactct ataatatgtg agggggttac attgatgagt
       61 agactacata tgggtattga tgtaggctca acaacagtta aagttgttgt gcttgatgaa
      121 aacaataata ctttatttgc agaatacaga cgtcattttt cagatataaa aagcaccgtt
      181 aaaggccttt ttgcagaagt caaggaggtt gtcaaaaacg aannnnnnnn nnctattatt
      241 taccctatcg ccgctcgttg cggtgttttc gcaaaatcgg acgttcagcc gcttttaaac
      301 gagggtgcgg cacgtgaaga tattgccgcg tcaattcttc aagccgttgt aactcagaca
      361 atcagcggtc ttgcacaagg cagacctata aagggcaata tcgtatttct tggaggtcct
      421 ttgcacttct tacctcagct aagacgtcag tttgaggaaa ctttaaagga caatgcaaag
      481 tctttcaaaa ctcctgaaaa cgcacagctg ttcgtggcaa tcggtgcggc acttatgtac
      541 gaagacaaga cttccgacat agacacactt atttcacagc ttgcaaacgc aaaatctgtt
      601 gacgccgaaa ttacgcgtat gcgtaagctg tttgaaacag aggacgaacg caaggaattt
      661 ttcgacagac acggcaagga cgttgcaaga cgtaaggata tttcaaccgc aaacggtcct
      721 gtattccttg gtcttgacgt cggttcaacg actatcaaag ccgcacttat taacgaggac
      781 ggcgatattc tttatgatta ttacggcaaa aacgaaggca gtcctattac ctgcggtata
      841 aaaatacttc gtgaattgta ttcaaaattg ccgaaatcgg cttatatcgc aaatgcctgt
      901 acaacaggtt acggcgaact tttgttaaag actgctttca gaattgaaga aggcgaaatc
      961 gaaactatcg cccactacaa agccgcaaat tatttttcac cgggtgttga ttttctcatc
     1021 gacatcggcg gtcaggatat gaaatgtatg aaaatcagaa acggcgtaat cgacagtatt
     1081 atgcttaacg aggcctgttc atcgggctgt ggttcattca ttcagacatt tgcggaaagt
     1141 ttgggacttg atacaatatc attctcacaa gaggcacttg tggcagacaa tcccgttgac
     1201 cttggtacgc gttgtacggt gtttatgaat tcgcgtgtta aacaagctca aaaagagggt
     1261 gcgactgtcg gtgacatcag tgcaggtctt tcctattccg ttgtcagaaa cgctctttac
     1321 aaggttataa aacttcgtga ccccgaacaa atgggtacta atatcgttgt tcaaggcggt
     1381 actttcaaca acaacgctat acttcgttgt tttgaacttt tgacaggcaa aaatgttgtc
     1441 cgtccggata ttgcaggtat tatgggtgcg ttcggctcgg cgcttattgc aaaagagcgt
     1501 tggaacggcg gtgaatgtga tattcttcgt gttgatgaac ttgattcgtt cacattcgat
     1561 acttcactta cacgttgtca aaagtgtaac aaccactgtc agcttacaat aacaaaattt
     1621 tctgacggaa gtcagtttgt atccggtaac cgttgtgaac gcggtgccgg acttgaaaaa
     1681 gccaaaaagc aaatgcctaa tctttatgat tataaataca aacgtgcatt tgcatacaag
     1741 ccgttaaagg cagagaatgc accaaacggt gttatcggta taccgcgtgt acttaatata
     1801 tatgaaaact atccgctatg gtttacattc tttactgagc ttggtttctc cgtaagaatt
     1861 tccggcagaa gtacgcacga cctttacgaa aaaggtatcg actcgatacc gagtgaaagt
     1921 gcttgctacc ctgcaaaact tgtgcacggt cacattcaag accttatcga caagggcata
     1981 aaaactattt tttacccttg tattccgtat gaacagatag aatattcgga cgcaggcaat
     2041 cacttcaact gtcctatggt aacatcttat ccggaagtta tctacaacaa tatggacgtt
     2101 gtacgtcaaa gcgatattaa cttcttatat ccgtttatca accttgccga caagccgtcg
     2161 tttaaacgtc aaatgatgaa agcgttgcac gattacagct ttacaaaaac agaaattgac
     2221 aacgcgtctg aaaaggcttt tgccgaactt cagcattaca agcaagacat cagaaataag
     2281 ggtgaagaag ttttgaagtg gcttgacgaa cacgatgaac gtgctatcgt acttgccggc
     2341 agaccgtatc atatcgaccc tgaaatcaat cacggtattc cggatatgat tacatcactc
     2401 ggctttgccg ttcttacaga ggacagcgtt gcacatctcg gtcatcttga acgtgatatc
     2461 agagttgttg accaatgggc ataccataca agactttacg aatcagcggc acaggttatc
     2521 aaaaatccgc gtcttgagct aattcagctt aattcgttcg gctgcggtct tgacgcagtt
     2581 acgaccgacc aagtacagga aattttgcag tcacacgaaa agatttatac gacattgaaa
     2641 atagatgagg tttcaaatct cggtactgca agaataagag ttcgttcgct taaagcggca
     2701 gtcaaggaac gtgacgataa cgactttatt ccgcataaaa ttgatgatta cacaattaac
     2761 agagttaagt ttacagagga agaacgcaaa aagcatacta taatcttccc tcaaatgagt
     2821 cctacccact tcggactttt cgaggcggtt atgcgtgcaa acggttacaa tgcacttgtg
     2881 cttgaacacg caacggcaga ggacatcgag gctggtttaa agagcgttaa taacgacgct
     2941 tgttatccgt caattatggt aacaggtcag cttgtcaatg cgtttacaag cggcaaatgc
     3001 gacccgaaca acacttccgt tatgattaca caaacaggcg gcggctgtcg tgcgactaac
     3061 tatatcgcct tccttcgtaa ggcgcttaaa gaggcaggct atccgaatgt tcccgtaatt
     3121 tcgcttaacg tgtcgggact tgagggcaat cccggattta agattactcc gaagcttgtt
     3181 gacggtcttt taaaggcttg tacttggggt gacttattgc ttgccgttac acttcgtatc
     3241 cgtccttacg aggtaaatga gggcgaaaca aatgcggtac acgaaaagtg gcaaaagcgt
     3301 ttgtacagcg atattattaa taataaccgt aaacagctta aacttaaaaa ggttattgat
     3361 gaaataatcg cagactttga cgcaatcgaa attgatgaaa cagtcaaaaa gccaaaagtc
     3421 ggtgtggtag gtgaaattct tgttaagttc caccctgacg caaataacaa tattattgat
     3481 gttattgagt cggagggcgg tgaggcagtt atgccgggac ttgcggacat tatgctttat
     3541 tgtttgtata acaataactt caaacgtgat aatcttggtt ctaagcgttc aaccgccgca
     3601 ctttgcaatc ttgcaatttg gggtattgaa agtttcagaa agcatatggc aaaggttatg
     3661 aataacaatc ctaagttccg tatgcgttca ccgaaacata ttgcggatat tgccgagggt
     3721 gcaaagcacg ttttacagct tggtcactgc acaggtgagg gttggttcct gacaggcgaa
     3781 atgattgagc ttataaattc gggtgttccg aatatcgctt gtgttcagcc gtttgcttgc
     3841 ttgcctaacc acgttacagg taagggtatg attaaggaac ttcgcagaca gtacccgcaa
     3901 gccaatatcg ttgcggttga ctatgacccg ggtgcaagtg aagttaatca gcttaaccgt
     3961 attaaactta tgcttgcagt cgcttttgaa aatatgaaaa aagacgaaat gtacgactct
     4021 gttatgccaa agaaaacaga agtcagagaa actgttaaaa tagaaaagta acataaaaaa
     4081 agcacctttc ggtgcttttt tttgttattc tgcttttaca taccgtcccg caaaatatat
     4141 atttccgcta tcgtccaata tgtaatatgt aaacggttta tcggctttaa attctattgg
     4201 ttcttccgac cgataacctg atattttagt gataatcaca gttgcggcag ccgcctctgt
     4261 tcccttttca tcaacat
//
LOCUS       DAWACS010000076         4256 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2261_length_4256_cov_3.286836, whole genome shotgun sequence.
ACCESSION   DAWACS010000076 DAWACS010000000
VERSION     DAWACS010000076.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2261_length_4256_cov_3.286836"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1216 a          962 c          744 g         1334 t
ORIGIN      
        1 ctattgtact atacaacatc gtttctgtca agccaaagta tatgagctgt cgatattttg
       61 tcgacagctc ataaaatcac atcttgcaat gtattttttg agtattttca gagaaaaaat
      121 ctaaattggc attattttct cttgaaaaac attttattcg gcaatgcaaa cacaagtaca
      181 atccccaccg caatcgccac ggcaactttt attgtatcaa cacctgtgta cattgctttc
      241 atatagtccg acgctatgat gttataaaca gtgttataca tacccgcacc gggtacaagc
      301 gatattattc ccgaaatcaa ataaaccgta acgggcatac gtcttatgtt cgccaaaatc
      361 cgcgacaaca ccgtcacggc aatcgacgca acaaatatag caagtgccgt ttcgtcgctt
      421 attctaagtg taaacaaata caccccctct gcgactaaac ctgcgattcc gcaaaatatc
      481 aattcacttc ggctcacgtt aaatatcact gcaaaagcca ttgtcgccgc aaaagcgaca
      541 aacatttcaa gaaaaatcat cccataatac ctcccgtcat atgaccgaaa atgtacataa
      601 cactgcccgc acctgtggca agtgcaatcg ccacaactac cgcatcaaac aatctcgcac
      661 cgcccgaaag gtaatctccg gcaataatat cacgaattgc attagttatt gccaaaccgg
      721 gtacaagcgg cataatagct cccgttatca taggattaat attttttccc aaacctattg
      781 cataaaagag taatatcagc aacgcaataa caactccgcc cgaaaatgtt ataataaact
      841 ccgccgtgtg atgttttctc aaaaaccata taaaaacatt gataaccgca ccgaccacaa
      901 acgaattaag tccgtcaaac gcattaccgc caaacaacaa cgtaaaaaat ccacagcaaa
      961 acgctaccgc aacagttttc acccataacg aataaggttt ctgtgccatt atatattcaa
     1021 agcgttttaa agcttcttca aagtcgattt tatcatcagc aatatctcgt gacatcatat
     1081 taattttcga tactttatca aggtttatag tccgtctgcg aactctgacc gtttttgtaa
     1141 catcgccgac agtgacaaac agtcctgtca ttacgaccaa aacctcactt ttaccgtcat
     1201 aaaaatgccg tattatcctc tcaaccgtat cttcgacacg actcgtttcc gcaccgcttt
     1261 caagcagacc tctgccgaca tccgccgcaa atgtcacaag ttcatttatc tgcatattct
     1321 catctcctca cacaatatgc agacaacgcc aaaacttata tatcctttac cgaattaacc
     1381 tttaatacaa aaagtattga cgctcctaca cttataagta ttgttcccac tataaccata
     1441 atatttacct ccgtttttgt caaggtgatc ttagaaaatt tctcgttttt taccctcaaa
     1501 accccaactt cactttgaca atttagcttt attatgatta tattataaca actacaatgt
     1561 ccaaaaaacc gtacacaaaa agatggcatt ttcatgccat ctttggtata gtgaacaaat
     1621 tttaaatact gaattattca gcaaacatag gagttgacaa atatctttca cctgtatcag
     1681 gcaaaagagc tacgattgtt ttacccttgt tttcagggcg tttggcaagc tctgtagccg
     1741 cccaaacagc tgcacctgat gaaataccta caagtacacc ttcactgcgg gcaatatctc
     1801 tgcctgtctt gaatgcgtcc tcgttttcaa cagtaatgat ttcgtcataa acctttgtat
     1861 caagagtttc aggaacgaaa cctgcaccga taccttgaat tttgtgcgca cctgctttgc
     1921 cttgtgacaa taccggtgag cctgcaggct caactgcaac aaccttaacg tcagggtttt
     1981 gtgactttag atactgacct acaccggtaa ctgtaccgcc tgtacctacg ccggcaacaa
     2041 atatatcaac tttaccgtct gtatctgccc agatttcagg acctgttgtt gctctgtgat
     2101 aagcagggtt tgcagggttt gtgaactggc ttgggataaa gctgttttct gttgtttcag
     2161 caagttcctt agccttttcg atagcgccct tcataccctt tgcaccgtct gtcaaaacaa
     2221 gttcggcgcc gtatgctttt aatagattac gtctttcaat actcattgtt tcaggcattg
     2281 tgattataat tttataacct cttgcggctg caaccgacgc aagaccgata cctgtattac
     2341 ctgatgttgg ctcgatgata acagaacctt cctttaactt accctcttgt tcagcctcat
     2401 caaccattgc ctttgcgatt ctgtctttta ccgaaccggc agggttaaag tattcaagtt
     2461 taccaagaac ggttgcttca aggttattct tcttttcata gtttgcaagt tccaatagcg
     2521 gtgtaccacc gataagatct gtaattttct gatatatctt tgacatttta attccttctt
     2581 tctgactttt aacacaattt acttaaaatt agtcaacggc gttgaatgcg tcgtcaagag
     2641 ctgcaataag gtcatcaata ttttctgtac cgattgatag tcttattgta tttggcttaa
     2701 taccctgttc tgcaagttcc tgttcattgc actgtgaatg agtagttgta gccgggtgaa
     2761 ttacaagtga cttagagtct gcaacatttg caagaagcga gaaaatttca aggttatcga
     2821 taaacttgtg agcctcttca actccgccct tgatttcaaa tgtaaagata cttccgccgc
     2881 cgtttggaag atacttcttg tatagttcgt ggcttggctc tgtaggtagt gacggatgat
     2941 gaacagcctc tacctttgga tgcttgttta ggtaatcaac aatctttaga gcgttttcaa
     3001 cgtgacgttc aactcttagt gatagagttt caagaccctg taagaagatg aatgcgtgga
     3061 acggtgaaag tgtagcacct gtgtcacgaa gtaggatagc tctgatatat gttacgaatg
     3121 cggcagctcc tacatcattt gcaaagctga taccgtgata tgatgcgtta ggctctacaa
     3181 ggtgagggaa cttacccgac ttagcccagt caaagttacc gctgtcaacg attacaccac
     3241 cgattgcagt accgtgaccg ccgataaact tagttgcact gtgaacaacg atgtctgcac
     3301 cgtattcgat tggtcttaca agataaggtg tagcgaatgt tgagtcaact acaagcggaa
     3361 tgttatgtgc gtgagcaatc tttgcaattt cttcaatatc aattaggttt gaatttgggt
     3421 taccaagtgt ttcaatgaag attgcctttg tgttatcctg aatagctgct tcaaatgcac
     3481 cgtcaacttc agggtcaacg aatgttgttg taacaccgaa ttcagggaat gtatgttcaa
     3541 gatagttgta tgttccgccg taaattgtct ttgcggctac gatgtgatcg ccttgcttgg
     3601 caagtgtttg gaatgtataa gcaagagctg ccgcacccga tgcaactgcc aatgctgcaa
     3661 caccgccttc aagggctgct attctctgtt caaaaatatc ttctgtaggg tttgtaagtc
     3721 tgccgtagat gttacctgca tctctcaaac cgaaacggtc agctgcgtgc tgtgagttgt
     3781 ggaatacgaa tgatgttgat gcataaatcg gaacagctct tgaatctgta acaggatctg
     3841 cttgttcctg accaacgtgt aattgctttg tttcaaatct ccaattctta tttgacataa
     3901 ttttgtacct ctttctttct caatttttat atttatgtgt attgttcttt cctattaaat
     3961 tagttacagc aacagcaaca cattcgtccg ttctgtacat actttagatt ttgtgttatc
     4021 atatcgcatt ttcctttctt ctcttttcct atggaattac tatgatttgt attatagcac
     4081 atttttccga tttgtcaata cctttttgaa aaatttttta taaaaaataa atatccgcta
     4141 aaattcttcg attttatgcg gatatttacc tctgaaatat tatcaaaaat catattgtat
     4201 atgttcaaat tgctgacaaa actgttatgg ttttgtctac tgtattcttg gaggag
//
LOCUS       DAWACS010000077         4207 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2288_length_4207_cov_2.050337, whole genome shotgun sequence.
ACCESSION   DAWACS010000077 DAWACS010000000
VERSION     DAWACS010000077.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2288_length_4207_cov_2.050337"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2351..2360
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1171 a          958 c          695 g         1373 t
ORIGIN      
        1 accgaacttt tcttcaaagt tatcaagctg aggttttgta ccctcgcccc atacccatat
       61 tgatgttgca ggatttttgc cctctgcaac acgctttttg tttacaggat gttctcttaa
      121 aatcttttca ctttttttca tcatttcaag aagtgtatcc gcaccgtcac cctttggaag
      181 atggtctgtt atcttcttgt ctgaaatgtc gtgaggaggt gttaattcaa cattagtcga
      241 accgccctcc caaactgtaa gatgacggta gctgataccc ggatagaact taatcatatc
      301 agtatcaagc tcttttgcaa cagccttgat aagctcggca gcctcttctg ttgtgatttc
      361 gcctgcactg taatcaagca ttgtcttatc ctcgtaattt tcttcgtcag aaagagttac
      421 aaggttacat ctgaatgtaa cgtcattatc ctttagcgga acgccgattg acgccgcctc
      481 aagcggtgaa cgaccgctgt aacatttttt tgtatcataa cccataacag atagattggc
      541 tacgtcactg cccggtttca taccgtcttg aacagtctta accataccca tctcgccatg
      601 ctgtgccata tagtcgattg taggcttatc agccacctct aaaattgttt taccgtcaaa
      661 tgcgtcaacg ggataatctg ccataccgtc gccaagtatt actacatact tcatttttta
      721 tcttcctttc attttatctt tatgtttaaa gtttattcag tcacgatttt caccaatatt
      781 gctattgccc cacgtccgac gtgagtaccg ataagcggac cgaattcacc atacatttct
      841 atacaatctt cgccgtattc atcgcgaagt ttttcacaaa cctcctgtcc cttatcctcg
      901 tccgactgaa cgattaagaa cttaggattt tccgcgtcaa aatcagaatc atcttgaatt
      961 tttgcaatca atttatcaag cagctttttc tttcctctca gcttgtctaa actttcaaca
     1021 agtccgtgtt ctattgtaag tattggcttg atgtcaagca ttgtgccgac aaatgcggct
     1081 gcttttgaaa gtctgccgcc cttttcaaga tattttaacg tatcaactaa aaggtaacat
     1141 ctccatgttt ttatgtactt ttcacattcc gttataatct cgtcaacgct tttgccgtcc
     1201 ttagccattt gcgccgcatg aacggcggtt tgtgcaatgt acagagaaaa cttctgcgta
     1261 tcgacaatgt gaaaatccgc attcggattt tcttcttcaa tctcactctt aacaagattt
     1321 gctgtttggt actgaccgct tgcggctgat gaaagtgcaa aatatattac gctctcatgc
     1381 tcctcacact gctgtttaaa gtagtcaagc atatcaccga acggtgtctg tgaagtagtc
     1441 ggaataccgt cgtactcctc gagtttatca aaaaattgct catttgttat ttcagttctc
     1501 tgaacatatt gttcagtgcc gaaaatactt aaaaatgata taacgcctat attatatttg
     1561 tcggctatat cctgcggaat gtccgaaccg gaatccacta ttatttttat atctgccata
     1621 tttgctcctc tcatataaat tcggtataaa taaaatttaa aattgttttt tattatcttg
     1681 attaaaggct ttagacgcag agaaaattgt tcacagtgcc gttttgcagc tcacggtgtt
     1741 gtacgttacc gccttgagca aaaaatggtg ctgtgggcaa tttaatcaag tctaattacc
     1801 aatggaattg agtcagctgt cctaaatcct ttaaattatc aaacccttgc tgacgaagtg
     1861 cctcgtatac aacaatcgca acggaatttg aaagattaag acttctcgcc tcttctatca
     1921 taggtattct tatacaacgt tcttcgtgtt cgtacaaaag ttcttcggga agtcctgcat
     1981 cctcacgacc gaacaatata taatcgttat ccttaaattc agcctcaaca tatgtatgag
     2041 gtgcttttgt tgttgcataa aaatatcttg caccctctgt tttttcaaaa aagtccgcca
     2101 aattatcgta atagcataca ttcagcaaat gccaataatc caaacccgct cttttcaaat
     2161 ttttatcggt aatctgaaaa gccgtcggac gtacaatatg aagtctacac cctgtcgccg
     2221 cacaagtacg ggcaatgttg cctgtattct gcggtattct cggttcaaca agaactacat
     2281 taaacaaaaa atcatatcct ttactttaga aatttaatca taaatctgtt taacagcggt
     2341 cgttttatct nnnnnnnnnn aagttcttga cagcaaaaac attttataca ccttttcgtg
     2401 tcaataacag gtttacccga tgacatatca atcgctttcg gcggacaaca tctcgcacat
     2461 tctccgcaac ctacgcatat atcgtaatcc attgcaggct ttgacgcaag tgcatttata
     2521 agtcgtgcat taagtgcgtc cggaagtgaa atcaatttaa tcaaattaaa atggctttca
     2581 ggctttaaat aatccttcat aacaagcggt ttaatatctt cgcccgctat atcaattttt
     2641 tccgcactgt cacacacaag tccgcgttca atcgcctcac gcaccgtatc aacttcattt
     2701 ggcgtgtaat caatcaaata gcatgacgcc aaatcaagcg cgtgtgcatt ttctgatgcc
     2761 ataacaagtc ctatatgtct gttctgacct gccgtcggac cgttgccctc cataccccaa
     2821 acagcgtcca taatggacaa tgtcggtttt acacattcgt gcaaatccac aagcattgaa
     2881 cagaatgact ttctctcatt caatctgaaa tgcagttccg ctttatatgt tcccggaatt
     2941 gtgccgaaaa gatttttaac ggcacctgtg taactcgtca ttgcgtgagt tttcagtttc
     3001 ggcagtgaaa ttataacatc tgcattcaaa atcggattta ttatcggaat tttctttacg
     3061 gtctttccct cagggaaatg cacttccgta aaacttgtat caaaattcaa ttttgcgttt
     3121 gttccctcga tagccttatc cactccgcag actgaataaa caccctttaa agccgcagta
     3181 ttatacggac cgccgggact ttcggcaatc gtcacgtttc cgccggcttt ttcgaccatc
     3241 actataaccg cgtgaagaaa ttcgggattt gtcgtaaccg cctcctcagg ctttttcttg
     3301 gaaacaaggt tgggctttat aacaacatta tcaccctttt tcacaaactt cccaattcca
     3361 ccgattaaag acaccgcatt ttctacggca ttttgcactt cggaatatct gtcacatttt
     3421 acgacggaaa ctttattatt cattctttaa ttcctctctt aggtttctaa atgttgtaaa
     3481 cctctgccta ttctatcatt atacaatatt ccgagtaata tttcaaccgt aaaacagtaa
     3541 tttttatagg catagtgcca ttttattgca tatatatttc gctaaaatac ggcacactcc
     3601 attaaattat atcttttttc gttttataga cagtttgctc cctttaaagt aaaaaaaaga
     3661 cttgacataa agtcaaaagc agagtattat tttcacatat cgaaaatacg ttactttgat
     3721 gtaactttta aacggtgtat ttttggtata aaaaaaggtg atttgcaaaa atcacctttt
     3781 ttcaatatca gcttttttag ttttttggat tgacaatatc acgccaatca aggtcaccgc
     3841 gttcaagacc gtgaataagc acttctgccg ttgcacagtt cgttgcaatc gggatattgt
     3901 gcatatcgca aagtctcaga agcgaaaata cgtcaggctc atacccttcg gcattaagcg
     3961 gatcacggaa gaaaagcacc aagtcaattt cattatatgc aattctcgca ccgatttgct
     4021 gatcgccgcc ctgcggtcct gacaaaaatc tatatacatt aagaccggta ttttcaataa
     4081 tcatcttacc tgttgtgccg gttgcataca gagaatgttt tgacagaata cccgagtaag
     4141 caatacaaaa ctgtaccatt aattcttttt tcttatcata tgatattaaa gcgatgttca
     4201 cgaaaat
//
LOCUS       DAWACS010000078         4199 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2296_length_4199_cov_2.602317, whole genome shotgun sequence.
ACCESSION   DAWACS010000078 DAWACS010000000
VERSION     DAWACS010000078.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4199)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2296_length_4199_cov_2.602317"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1241 a          864 c          656 g         1438 t
ORIGIN      
        1 ttggttttaa acattgtcag gtaagtcgtt ttaaagtttt tacgctgcga taagaaacgt
       61 ctttcttagt aataacggcg gcaattgcaa tcgataaaag tgatatatga gcaattgtat
      121 taagattttt tacagaattg ccattacgaa caaaaactct ttcacagcct gcttgcttaa
      181 atcgtgaatt gtatctttcg gcttcggttc tgagagcata aacggattta aaggctattg
      241 agcttctatc gatagaaagc ctgtaatcat cgggaagagt tatgtattta gtacaaccgc
      301 gatttttctt gccattattc caacatttgt gattacaagg gcaattgcca tttttagagc
      361 gtttcaacgg acagcagtat ttctgacgtg ttcgaccgct atctgaaaac ttgccgtctt
      421 tatgcattgc gagtcctgct tcgcaaatga tattccctga cggtaacttt ttagggcttt
      481 tggtatttcg tttgtttaag gcaataaagc aatcgccatt gtatgtgtca tgcacagtgt
      541 tgtaaatagc cttaacatca taggctttat cagctaaaaa tgagcattca gacataggaa
      601 gataagaatt tgctcttttg agtatttcga gtgttacaga actgtcggaa acattggcac
      661 cggttgttaa ttcacaaacg ggtaagcccg atatgcagtc gacaagaata tgatttttgt
      721 aaccccaata gaattcataa ttcttttcgt tatgctgatt tgaagcagtt tgaacaccaa
      781 gcctgcaatc tttatcagac tttggatgag aggctttgga gaacttattc tttttaaagg
      841 acttgggatt gttgtttgaa acattagcct taacaggagt agcatccaaa gcgataaaag
      901 atgaatcaat caaagataaa tcggcagctt tcaagacctg tgattgcata acagtttgaa
      961 gtatatcgtt atcaaattca cggataaatc gtgtgaattt tgcataagaa ggtaattcat
     1021 gagaaatgtc aaagccacag taatgggcaa taattaagtt gtttgacaga taatcgtgta
     1081 agtccgaaat ttgagagaaa ccttcgcatt tcataacgat aaaggcacaa atcatagaat
     1141 gattgctata acttgcacga ccgaattttg agttttgagc cggaatacag cttaaatcta
     1201 aattaatgaa tagttcatcg taaaatttag cttttggttg tgaagtgaaa agactgatgt
     1261 cttttaaaat ttcttgacga tagataaaaa ccactctcct ttgtgggatt ttgatttcaa
     1321 ctatattata ccaaatcaga gtggtttttt ctatacatta aatattaaat ttatagtgac
     1381 aaaacggatt tgccacatcc gtaaaattgc ataaactcta tgtttttaga gctttgcacg
     1441 tggctaatat tttttatact gttattgccc taatgagagc aaaattcccc aatgtttaaa
     1501 cacttcataa tttattcttt aaatttattt ccctcatcct ttcgctttgt agatatttag
     1561 attttattcc aaatatacaa aattatgtag tgataattaa atttattata tcattcttga
     1621 caaatagtgt caatatattc catagaatat atcttacaac caaaaacggt ttaaacattt
     1681 cgtttaaacc gtttcacatt ctaaatctta ttccacttca tattcaaccg tggcaagttc
     1741 attcatttcg atgtcataca gagttgccgt aacagttccg ctcggtgtaa aatcctttgg
     1801 attcatcact ctatattcac ctgcaccgtt tgtgattacc tttctgtctc cgacattatc
     1861 tccgcactta aatctgacta tataagaagc ttcttccgta acggtaatat aaacactgcc
     1921 gtctttataa gtaataccat actcccaatc tttcttcggc aatgtaatct tcacattacc
     1981 atattcagag gtatatagct catatccttc ttctgtcatt gtcgacggtt ttgtatactt
     2041 ttttgtccac accgattcgt ccgataaatt tgggattgtt ttttcttccg ttacattcgg
     2101 ataaccctca agcgaacgaa taatttttcc gctttcggtc aatgtcgggt cttcagatat
     2161 gctccaagca ccccaccaat gagcataaag cgtcacatca tcgcctgaaa tttcttcaaa
     2221 gttttcaact tttgttccac cgtctttttc ggtgtaccaa ccgccaaacg caaaattatt
     2281 tcttgtcaaa tcggcaaacg cctgctcgcc gtctgtccaa tagttgttaa tgtatttgca
     2341 gcttgttatg tttccgccac tataattagg gtcaaatgta acattgcgtt cccttatatc
     2401 attcggaagc ggaatagtct tcaccacatt cactatataa ggcatattgt ttttataata
     2461 atctcttaca acctcaatat tatttccttt ttgtcttatt cggcagaaaa caacatcttt
     2521 aatattcttg attgcatatg tataatattt atcggtttca aagctataaa gatacactgt
     2581 ggattgactc gttacaaagc tcggtctaaa gaaacaaata tatttgctgt cctcagttat
     2641 aatatagctt gtcggtttcg ctccgttttc ttctgatgaa ggtataagtg tttctgtaaa
     2701 atcagaagta gttttcactt tgtccatatc aatatagtag aatttctgaa tatcatcatt
     2761 ccatataaat tgcatataaa aaccggtaaa catatcatat tcttcataag tcgtatccgt
     2821 acaatgcgta gcataatctc catatcctaa tctgttcaat acagtatcac ttactaaaaa
     2881 gtacccacga ctcactgtgc ccagcatatc gggaacaggg tcatctgccg taacatcaac
     2941 atgatatttt tttccgccga ttgtaacgca gttccaagca tgaagttgcg gatcctcacc
     3001 caaatgcacc ggttttcttc ccaacacata cgttgccgtc actccgactc tgccaagtag
     3061 gtcgttatat gcattagcat aaccgtcgca aacggcataa ccctctacaa tcgcaccgta
     3121 cgcagtgtgt gcattcggtc ttgatgtcgt cacatcatac tgacaattca tcacaagcca
     3181 gtcgtgaagt gccaatgctt tttgaagttc cgtcatatcc ttgcccttat ttttgccgag
     3241 agcctcacga agtgctttat cgaccgccgc ctcatatatg gtttctttat cttcgtccga
     3301 aatgacatct gttaccgtaa cggcatctga aagcgggttc attgtttcta atccgtccca
     3361 atacataacc ttgtcgccgt tttttacatt aagcaccgta ctgctgcttt ccgtcacata
     3421 ctctttcaca acctttgtaa gttttccgtc ctcgtcatac gatgccacaa tcgcaacgcc
     3481 cgatgtaccg tttccgacaa ctgctttgcc atccactaca ataattgcca agtctgccgc
     3541 cgaaacactc accgacatga caaacaaacc tatcaacacc gcaattaccg atattacttt
     3601 cctataattt ttcatattta tccctccttt aaacgcatta atatcatatt cttttataat
     3661 aattatatca gaaattcata agattttcta ctgctttaat aaaaaaatat acaggtaagc
     3721 cttatttaaa atagacttac ctgtttcaca ttttatcttc tgtcgttctt tacacctgtc
     3781 ttataatcat aaaaagaata tgcctcgtta agagcaaaat ttcctaaagt tttaaagaca
     3841 tcatctttaa actcattttg tttcatttcg cccttttcat ttatatatct gtacatttcg
     3901 ccttgataaa aatacaaatt gttttgattt gccgccgaat aaacaagtcc aaaatacaat
     3961 acatcattat taaaataata aattcgatta tacttaaaac cgtttacacc tgacggaata
     4021 tctattctca gaacttgatc ttcgagagta taatacttta caccgccgtc aatctctgaa
     4081 acctgaagtg aaccaagact tttatctgta ttttctctca atttattgat tatgcttgat
     4141 ttcgttccga cgccttttat ctcagccgac ggcatattat tttcatatgt attaggtat
//
LOCUS       DAWACS010000079         4197 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2298_length_4197_cov_2.744809, whole genome shotgun sequence.
ACCESSION   DAWACS010000079 DAWACS010000000
VERSION     DAWACS010000079.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2298_length_4197_cov_2.744809"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    640..649
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1424 a          583 c          965 g         1215 t
ORIGIN      
        1 gtataatgga agataaatgt gcaagcatat tgactgaatt ttttagagag ttaagaaaaa
       61 agaaatagac tgttttttat attgcaattt atttacagat gtaatataat aaattttaag
      121 gggtgatttt taatgaaaat aagaaaaatt atatcagcta tacttactat gtctgttatg
      181 tcggcttgca taatacctat tgcagaagcg gacggattgt attcggaaaa attcgatatg
      241 acgaaagtaa aagcagacaa gacggacggt gttacgaaag aagtggaagt gcctgacggt
      301 gactacactg taacggtaac aaccggcgga aaaacagaaa caaatgcgaa tatttatata
      361 aacggcggtg aacgtgtaag agcgtacacg cttgaggcag gcgaaacaca ggaaaatgaa
      421 cagcctgttg tgccgaaaaa cggaaaaatc actgtacagg ttaagggtga caaccctaac
      481 gtgacggaaa tagaaataga acagttaccg acacgggaaa aagccgaaaa acctactata
      541 tatattgcag gtgattcaac ggcacaaact tacaactata caaaggtata ccctcaaaca
      601 ggttggggac aagtttttgc cgattatttt aatgacgatn nnnnnnnnng tgcaatgggc
      661 ggaagaagtt caaagagtta tgacaacgac ggcagacttg acagaatact tacagaaatg
      721 cacccgggtg actatgtgtt tattcagttc ggtataaatg acggagctga aaataaaccg
      781 gaaagataca taagtgttga ggactacaaa aagttaatta cagacaagta tatcggtgaa
      841 gttgaaaaac gcggcggaac accggtgctt atgactgcaa atgcggctgc ttggtgggac
      901 gaagaaaaca actgttttat ggagtcgcga aaagattacg cagaccctac gcgtgaaatc
      961 gcggaagaaa caggttgcaa atttattgat gaaaataaaa tcgtgacaga tgcgtggaac
     1021 agtatgagta agaatcgtgt tttgtcgggc tattttatat gtgagccgct tgaaagcaag
     1081 gcttatccgt caggtacaaa cgacaccact catatgaaag ccaaaggcgc aaagagagtt
     1141 gcaaagctta ttgcggatgc aattccggaa aatgtgcctg aactggcaaa gtatttaaag
     1201 ggtgacgaaa catttacgga tatacaaggt cattgggcgg aagatgttat aaaaacactt
     1261 gcagagaatg acaaagtatc gggagtcggt gacggcaagt ttaaccctga cggaactgta
     1321 acgcgtgcgg aatttttgaa aatggcgatg gattcgttgg gcatagtcgg tcacgcatat
     1381 cgtgacggcg agtgccttga cgcaacaaat gatgattggt attgctacta tttgcagggg
     1441 gcgttggata aggatattat tcctaaagaa atgattgaga actgcaacgt tacaaaagtt
     1501 actaaaactc ttgcagaagc aacagatgat aaagaggcgg ttacgactga cgtaaacgtt
     1561 tatacaggta agtttgacgg agataagccg attactcgtg aagaaatggc tgcgattgcg
     1621 gtttgttgca aaaattataa aatgagaaga ttgacagact gggacaatga aagacgttat
     1681 cctatttttt cgtttaagga cagtgatgaa attgacgaaa aatatataag tgatgtagta
     1741 gaagcttata atctgaacta tatagacggt atggaggacg gtcgctttgc accgaaagag
     1801 aatttgacgc gtgcacaggc ggcggttgta atgaataatt taaaatagga gaaataggaa
     1861 tgaatataaa gaaagtagtc gcagcggcat tgatattgtc gtcaatgacg gcggtatcgg
     1921 caagtgcggc tgaatttaac gatatcaaag gtcattgggc ggaagatgtt atcaatgagc
     1981 ttgccaacaa agatattata cacggtgttt cggatacaga atttaatcca gacggaactg
     2041 taacgcgtgc agagtttttg aagatggctt tgggtacagt cggtattgcc gatgtgccgt
     2101 acaggagcgg cgaatgtctt gatgttaccg cgtccgattg gtacggcggt tgtgtgcaaa
     2161 gtgcgctcga caaaggcttg atacctgaaa atatgattgg caactatgcc gcaaagataa
     2221 cagacggcaa ggctatttac agtggctttt ttgacggcga taagccgata aaacgtgaag
     2281 aaatggcata tatggtttat atgctgtatc aatacagttg tgatgaggag gagtcctata
     2341 atatacttac tcctgtcgat ttacagtttg atgatgtaag ccaaatatcg caatgggcat
     2401 tggacggcgt cagatatgcg tatacaaacg gcttaatatc gggtatggac gatgatacgt
     2461 tcagaccgca gcaaaccgca actcgtgcgc aggctgcgtc tattataagc agattacttg
     2521 ataaaacgga atagaggtgt aagaaatgtt tagcaaaaaa acaatcagaa ttatttgtct
     2581 tgttaccgca atagctatgg ttgtacctat ttgtataggt attatcagta tgtttgcagg
     2641 tgttaattaa tgtttaagga gaagtacata tgaagaaaaa aatcatatgg ttattttatt
     2701 tgttgtttgc aatatgtttt acttttactg caataaatat aatattgcat aatacatatt
     2761 ataaagcatt gcacttgatt tgtgttacaa caatatgctt agtcgggttg acaataatat
     2821 ataaaaacct ttccaaaaac gagaaattca ttgagaaaaa ttataataag atacttatct
     2881 catttggtat aggtatgttt ataatagaga ttgtattggg aattgcatta agatatgatc
     2941 cgttgtggga tgttggcgcg atacataaag gtgctattga atgggttgaa acagggacgt
     3001 ttgaaaatta ttatgaatat ttctatagat ttcctaacaa tcttgcggca atggcgtttt
     3061 tgcatttatt tttcaaaatt gcatcaatat ttggaattaa agactatttt gcggtttctg
     3121 tcgtaataaa cagtattatg gtaagctgta caacagtaat agtatcactg atttgcaaga
     3181 aaatagctga cgtaaaacat gccgtttttg cattggtact gtttggattc agtgtgcaat
     3241 tctatttttt gggcggtgct gtgtatactg atatgctttc tgttatgttt ccggtattat
     3301 ttttctattt atatctgaac tctaaagaac aatcgggaaa aagaaaaata attacatata
     3361 tattaatggg actgacaatt acaataggaa gtttgaataa attcactgta ataataattg
     3421 ctatagcagt aggcattgat ttgctcataa acagaaaatt caaggagttt acgaaaattc
     3481 ttgtcagcgt agtgagtatt atgatgatat ttaatgtaac atttaattta tatatgtatt
     3541 cttctcattt ggacagacaa acggccaagg gtgaaaatac accgtataat cattgggtca
     3601 tgatggggct taaaggtgat ggtgtatata atcctgagga ttatgagttt acaaggaaat
     3661 atgattacga tgaaaacaga aacagtaaga taaataaaga gattttcgat agaataaaaa
     3721 aattgggctt ttgtggtgta tttgatttgg caatgacaaa atcaacagca gatttcggaa
     3781 acggaacata tggtgctgaa gatttttata attgtaatcc tcaaaccaat acaaagttgc
     3841 atgattggat tttgcccgag gggaagaatt ataaaaagta tgcaacatat gcaacatcaa
     3901 tgcatattgc aatattaatt ttgatgttaa ctgctgcatg gggatttgta tgctctgatg
     3961 atgaaaaacg taaaaagatg ttttcattgt atttgacgat atttggagta tatttgtttt
     4021 tgctttgctg ggaaacaaac cgaagatatt tttcaaattt cgcacctgta atatttattt
     4081 gcggaacttt gggtattgat aaatttatag aggtttgttt gaaaactaaa gaaaaaatta
     4141 aatgtggttt aaaataggct gataaaggaa ctgtaacgat ttgttacagt tcttttt
//
LOCUS       DAWACS010000080         4183 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2303_length_4183_cov_2.719477, whole genome shotgun sequence.
ACCESSION   DAWACS010000080 DAWACS010000000
VERSION     DAWACS010000080.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2303_length_4183_cov_2.719477"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1309 a          687 c          973 g         1214 t
ORIGIN      
        1 aaattcatag gttttgacgc gtttaaggaa atcttcaaaa tgaaagattt ttacatagca
       61 cttcgtaata ctatcgtgct taacggtctt gatcttataa tcggttttcc ggctccgata
      121 attttggcga tattattaaa tgaaatacgc aataagtatt tcaaacgtat cagccaaacg
      181 gttttatatt taccgcattt tctttcgtgg gtaatcattg caggtatatt ctatcagctt
      241 ttgtcaccgt ctacgggatt tgtgaacgta cttataatgc gtcacggcgg tgaaagcata
      301 ccgttcctga cggaaaaatg gcattggctt gtgtcgtact gtttaatcgg cgtatggcag
      361 agcatgggtt ggggtacgat tatatatctt gccgcaatca caggtattaa tgctgacctt
      421 tacgaagcgg caacggttga cggtgcaggc agatggagaa aaatatggaa tataacactt
      481 ccttgtattc gttcgacaat cgttgtaatg ctgataatga gtttgggaag aattttgggt
      541 atatcgtttg aacgtccgta tacattggat aatccgcttg taagagattt ctcggacgtt
      601 atttcgacat tcgtttatcg tgtcggcttg cagtcgcaca gatataacat cgcaacggcg
      661 gttggtttgt tccagtcggt cgtaggactt atcttcgtat ttgcatcgga taagtttgca
      721 aaacttatgg gagaggacgg tataatataa tgaaaagaaa agctactgat gttttggcag
      781 atgtgtttat atatgtgttt atggctgtac tttcgctttt gtgtcttata ccgtttatac
      841 acgttctttc ggtttcaatc agcggtaacg gtgcggtaat gtcaaatcaa gtgttcctta
      901 ttcctaagga cgtcaatctt gacgcatata agacggtttt cggtgatgcg acaatgatgc
      961 gttcattgtg gtttacggtg tttgtgacgg ttgcgtttac ggcactcggt atgtttctta
     1021 caatatgcgg tgcgtttgca ctgtcaagaa aacgtctgaa gggcagaaaa gttataggcg
     1081 taatatttct tataacaatg tacttcactg ccggaacaat tcccgattac atacttatga
     1141 gccagttgca ccttataaat acggcggcgg ttttgatact tccgcttgca ttttcggcat
     1201 ataatctgat aatacttcgt acatttatgc aaaatagtgt accgcaaagc ttggaggagg
     1261 cggctgcgat tgacggctgt aacgacttta ttgtactggt tagaattgta ttgccgttgt
     1321 cggtgcctgt tttggcgaca cttgcactgt tctatgcggt aggtcgttgg aatacattcc
     1381 aagacgcact ttactttata acaaagtcat cgcttaaacc gttgcagtta aagttgtatg
     1441 agcttgttaa tgcggcaggt tcaaattcgt cggtttcgca ggaagtcgca ggggagagta
     1501 tgcaggctga ggaagttttg aaatcggctt gtattatgtt tgcaactatt cctatcgtaa
     1561 ttgtttatcc ttttatacaa aagtattttg ttaaaggtgt tatgataggt gcagtaaaag
     1621 aataatgaaa aaacatattt aaaaagagag gggaaaagaa aaatgaattt aaagaaagta
     1681 ttgtgtgctg cactaagcac agtgactgcg gtatctttgc ttgcgtcatg cggcggtaag
     1741 ggtaaaatgc agatggataa agcggagctt acaggcccga aagcctatga tgagcaaatt
     1801 gagcttaaaa tacctgtata tgacagaggt atgcagggac aggcagatgt agacaataac
     1861 tattggacgg attatgttca aaagaatttc ggtgacaagt acaatatcaa aatgacattt
     1921 gtacctgtta cgcgtaagga agatgtaacg gtatttaatg aattgcttgc ggcaggcgaa
     1981 gaacctgata ttcttttcag ctatgactat ccgacaatga tttcatatta ttcaagagga
     2041 gcgttccagc cgattgatga ggacgtgtta aagacatacg caccgacatt ctatgaaaag
     2101 acaaaggatt tggaggaata tacaaaggtt gacgaccaaa gatatttcct tgcggcaact
     2161 cgtccgttgg cttataactg ggtaactctt atccgtacag actggcttga aaaagcagga
     2221 ctttcaatgc cgacaaatca agaggaatat gtaaatgcac taaagaagtt taaggaatta
     2281 aagcttggcg gcgaaaatac aatccctgca acggagagcc tttacaacgc atatttcccg
     2341 aactatgaat acagagaata tccgctttcg gaagaagata acgcgatgta ttcggatatt
     2401 actgttgcat cgcttacata tgacgcaaca aagcagaagt taaagtatat gaatcagctt
     2461 tataacgacg gacttatcag ccctgaatgg taccttgaca aggacggcaa tcaaaagcaa
     2521 gctgattttg tatctggtaa agcaggcgtg ttcggtttct atttgtcaca aaatcctcct
     2581 gtacttcaaa cattattgca aaattgccct gacgcaaagg ttgcagtgct tgacgcaggt
     2641 gcaggttatc cggagggtac aaagcctgcc ggcagagcag attggccgtt cggtatggta
     2701 agcggtatca gtgttgactg tgaacaccct gaggcagtgc taatgtattt tgagtggctt
     2761 gcacaagatg aaaacctgtt cgtaatgcaa aatggtatcg aaaacgttac atataaagta
     2821 gaggacgaaa ttcctgtact tattgacgat tacacaggtg aagaaagatt gaactacaat
     2881 tcaaataaag atatgtggtg ccttgtaaca gagggtaagg actacggcag tgacgagaaa
     2941 aaccttgccg tacaaaagaa aacatacgca ccggcgggat ttgaggactt aattcaacag
     3001 tcatatgacg gttatcaaaa gactaaggaa tataagtata cagatttctt gttcgacaga
     3061 tcaatcgaca gcctttcaca gtatgccgaa actttaaaga gtaagtggga agttattcag
     3121 gttgatttga ttaactgtaa gcctgaagaa tttgacgcga agtatgaggc tgcttgcaag
     3181 gaataccttg acgcaggtta tcaagctgtt cttgatgaaa aagaacaggc ttatgccgat
     3241 atgaaaaaat aaaaaccaaa agggataatc gttaagatta tcccttttta gttggtaaaa
     3301 taattgacag tacagaactt aaatgataga ataatgttat aaatttatta aagaaaatag
     3361 ggagaaataa tgaataagaa aactgtttca aaagtaaatt tgtttttcgg cggacttgtt
     3421 tcgagaatgg aagaaaacaa agattttttt aatgatatta ctattaattt aaagtcggga
     3481 actaagaatt ttcctgcaaa aatattgtat acggacgata taactcttga ttatcaagcg
     3541 gttaaacgca aagtggaaaa agacggtttg tgggatataa tcgcggaaga attgcttaaa
     3601 tatgacgagg cgcagataat ttacagcgaa cgcggtgcga agattgtaat tgacgcaaac
     3661 gataaaaaag tcaatatgcg tcacgaggac gttacgaaag agataaataa gacgagcgtt
     3721 gtgcagacgg gcagagagta tgtcgtaaat cccgcacttg cgaaagattt gcttgaagaa
     3781 ataggcatac ttgccaagaa cggcaagatt aaaaatgata aaatccgtaa gtataatcag
     3841 atagattatt ttgttgaatt aatgcagggt gtgcttaaag aaatcggtga cagggacgaa
     3901 tatgttattt ttgacgcggc ttgcggtaaa agttatttgt cgttcgttat gaatttctat
     3961 ttgaaagaaa tacttaaaaa gaagtgtcat tttatcggtg ttgattattc cgaaacggta
     4021 attgaagcgt caaagagaat ggctaaaaat ctcggataca acaatatgga gttcatcaag
     4081 gctgacctta cggagtatac accgcctata agacccgata tagtcgtgtc gctccacgct
     4141 tgcgataccg caacggatat ggcacttgca cttggtataa gag
//
LOCUS       DAWACS010000081         4181 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2305_length_4181_cov_2.351188, whole genome shotgun sequence.
ACCESSION   DAWACS010000081 DAWACS010000000
VERSION     DAWACS010000081.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2305_length_4181_cov_2.351188"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2074..2083
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1113 a          963 c          630 g         1465 t
ORIGIN      
        1 atatcacaaa ataattttat tatctacatt ctgcctcaaa aattcttgac aattatttca
       61 ttatagacta tactatctgt taaggataca atatttatac tacctaatgg cgcctttgtt
      121 gtgccgtttc gtatagatat tgtatccttt attatttaac tgaaatatat tccgtaagaa
      181 aaaaacgtgt aaaaacgtgt acagcctatt taaaacaaaa acaaaccgcc aattttagcg
      241 gtttgttttt taattggtgg aggtgaggag aatcgaactc ctgtccgaaa acatatccac
      301 ccaagtttct ccgagtgcag gttatggtca atattccctt tgatactact ccacaaccaa
      361 agttatatca tcggtagtcc ttaaatcatg gaaaaggtca ggacatccct ttactcacgt
      421 tcaccactaa aatgatgcct tgccctatgc cgtggtactc acaggggaga caacgccgca
      481 attaggcagc gtaagctaat tctgtatcgt tagcgtttat atttaaaagt ttgagccgtt
      541 ttaagtggca ctcaacacta ctcgctactc gggattcaac atccccgtcg aaaccattgc
      601 acccccatat taataacggt tataattttt tatcgaccgc tcaatattac gttttgcgtc
      661 tttctttgca atatcttcac gcttatcata aagtttttta ccctttgcaa ctgccaaaga
      721 tactttaacc aaagagcctt tcaaatacac tgcaagagga attaaagcat aaccgtcctg
      781 ctgcatttgg tttgtaagtt tcaatatttc tttcttatga agaagaagtt ttctgtccct
      841 aagagggtca cgattaaata tatttccctg ttcaaaaggg cttatattca tctgctttat
      901 aaatacttcc ccgtttttga ttgacgcata cgcgtcggta agattaacct tacccaatct
      961 tgcagattta acttctgttc cggctaattc tatgcctgcc tctattgttt ccaaaataaa
     1021 atactcgtga cgtgcttttt tattctgtgc aagagttttt gtattttctt ttttatccgc
     1081 catagaaatt ctcctttcgt caaactgctt tgatattata acatatccta aaacaaattg
     1141 caaacaaaat catatattaa gattttctct cattatgcaa aatatgcttt ttttcgccga
     1201 ctttatataa tttactctca tacgcattat tttcatcgta aacaagcttc ggtacagatg
     1261 acggcttacc gttttcatca agcgaaacca tcgtaaaaaa tgcagtcagt gcaatctgtt
     1321 tattacctgt aataaggtcc tccgactcga ctgtcacaaa gacctccatt gacgaacgtc
     1381 ccgcatatat gacctgtgcc ttacacgtta caagctcgcc cacaagcacc ggctttttaa
     1441 aattaagctc gtcaactcgt gcggttacaa cgtttgtgtg ggcgtgtttc tgtgccgcaa
     1501 cccctgccgc actgtccatc attttcataa tttcaccacc atgcacattc cccgccggat
     1561 tagcctgcat tggcaataca agctgtgcaa gtatcgaatg tgagtgtttc attgtctgtg
     1621 attccattat gccgcctccc tactgcacat ttttaattat ttttagcatt ttcaagcatt
     1681 tgctttgcgt gacttaaagc aagttccgta acctcgccgc catcgattat tctcgcaagt
     1741 tcaagttctc tgccttcttc gtcaagctct tttaaagttg tagacgcaag ctcgccgtct
     1801 gtattcttct gtatcaaata atgattatcc gccattgccg taagctgcgg aaggtgtgta
     1861 atacaaataa cctgcttgtt ttttgcaata accgcaagtt ttttcgcaat actcattgcc
     1921 gccttaccgg aaacaccggt atcaatttca tcgaatatca ttgtatcaac gccgtccgaa
     1981 tcagccaaaa tcgactttat cgcaagcatt actcttgaca attcaccgcc cgacgcaatt
     2041 ttcacaagcg gttttaacgg ctcacccgga tttnnnnnnn nnntttgtgc tgattaaaaa
     2101 ctcaacctta tccataccgt tatcgtaaaa tgtaccgtca ttctctatat taaccttaaa
     2161 tttggatttt tccatattaa gctcatgcaa cgacttttcg atattttctt caagaacttt
     2221 tgcggcattt tcacgtcttt gcgtaacaac attgcccttt tctttaaggt ctttcgttat
     2281 gcttttaagt tcttctttta gttcgtttgt tctctcatca cttagcttta tatcattaag
     2341 ttcgctttcg gcttttttca aatattcaag tatttcttca atactgtttc cgtatttgcg
     2401 tttcaatctt gaaatcaaat caagtcgttc ttcaatttcg tttaaagcct gctcatcaaa
     2461 ttcaaccgtt tcgccgaact cttttatttc gtgtgccgta tcttcaagcg aatacataat
     2521 ttcatttaaa gtatcataaa tcgatttaag ttgaggattt aagtcgctta tctgagaaat
     2581 ctcattcacg gcaatgctta taccgtcata tgcggattgt atttcatcac cctcatacaa
     2641 gttcatatac gcctcattta cggactttgt tatctgctcc gcattagtgt atatatcacg
     2701 ttgttctctt aaatcgtcct cctcgccttt ttcaagcgac gcttttttta tttccttaac
     2761 ttgatattca agcaagtcaa ttctctgcat tttttcctgc tcgtccattt caagtgaaga
     2821 aatcttcttt tctatttcac gtttttttga taatatgtcc ttatacctgt ttatatattc
     2881 ctcattatca gcatacgcgt ccaaaaacgt aatatgtctt atcggcgtca aaagtgcctg
     2941 attatcgtgc tgaccgtgaa tattaataag tctgtccgat atttcacgaa gtatgttaag
     3001 cgttacaacc attccgttta ttctcgctgc gctttttccc tcttttgtaa cttgtcgtgt
     3061 aattataaca gtttcgtcct caacgtcaat atcgttttct tcaagaatat ttataacact
     3121 tttaggtgcg tcaaaaacag cctgtacaac agccttatcc gtaccgtatc ttacaagctc
     3181 tttatttgct ctgtcgccca aaatcatatt tatcgaatct attataatag acttacctgc
     3241 accggtttca cctgtaagca cacttacacc gtcatgcaaa tttatgtcta gtttatctat
     3301 caccgctaca tttctgatag aaagctggtt taacatactt tgtaatactc cttactgaaa
     3361 taattgaatc aatttttctg caatttccgc tgccttttct tcatttcttg caattatgag
     3421 caatgtatca tcaccggcaa tagaacccaa gaactcgtta cgcatagacg catcaacaga
     3481 cgccgcaaca gccgacgcca ttcccggata tgttttcaca acgatattat tcattgcata
     3541 atcaacactt attaccgtat cggtaatcat atttatacgt ctgtcaagtt cctccggaat
     3601 atccttagaa gatgaataaa cacttccacc gtcaggcaac ggtattttta taagtccaag
     3661 ttccttaata tctcttgata cagtcgcttg tgtaacatta tacccgcact tattgagttc
     3721 acctataagt tgttcgtgag tcttgatatt cttctcatgt attattctga gaatttgacc
     3781 ctgtcttttg tacttcaact cttatcctcc ctaattacga taacttttta attaatgtgt
     3841 cgtaaaacga ctgttcacct atttttatta gttcaaaatc atattcggat tcggtaatta
     3901 ttacttcgtc cgcatttttt atatatccct gaatgtcacc gtccgccgta acaaccgcgt
     3961 ccgcatttcc gtattctccg tcaagcttta ttcttatatc cttatttgac gaaagtaccg
     4021 cacttctgac agacaacata tgtgcacata tcggagtcgc aacatacaat ctcatacaag
     4081 ggtctaccac cggtccgccg gctgatattg aataacccgt agagcctgtc ggtgttgcga
     4141 ttataagtcc gtcggcggtg tatctgttta ctgctacacc g
//
LOCUS       DAWACS010000082         4174 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2310_length_4174_cov_3.114834, whole genome shotgun sequence.
ACCESSION   DAWACS010000082 DAWACS010000000
VERSION     DAWACS010000082.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2310_length_4174_cov_3.114834"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1124 a          972 c          679 g         1399 t
ORIGIN      
        1 ctgccagcgt gtagacactt ttgtctacac gctgaagcca cgagtaaact cgtggctttt
       61 atattactta atttttaaat tatctgcctg gtttaaagaa gtcgggaaca tctatactac
      121 ccattcccgg ctttaacttt ctcttgaaaa ttgcattatc atcataatca gaaactgatg
      181 aagaataaga cggagtcttt ctgtcgcttg atgttgaaga acctgtaaac ttaggtgtaa
      241 tattctttac atcattagcc ttatccttac cgcgtcttaa tgaaccgtca agacctgttg
      301 caataagagt aaccttaatt tcatctttaa gattatcgtc catagcagta ccgacaataa
      361 tatttgcctc ttcacttaca aggtcacgaa caatgcttga tacttcgccc atatcaacaa
      421 gtgagaattc acttccgcct gtgatattaa gaagtacgtt ttcagcaccc tcaatgcttg
      481 tttcaagaag cggactttca atagccgcac gaactgcgtc ctgagcggca ttttcaccct
      541 tgccgacacc gatacccata tgtgcgacac cgctgtcttt cataattgtt cttacatcgg
      601 caaagtcaca gttcataata cctctttgag taattacttc aatgatacct tgaacacctt
      661 gacgaagaac atcatctgca agtttgaatg aatctttaat tgtaaccttt ttgtctgcaa
      721 tcttcaatag aagatcgttt ggtatagtaa caatagcgtc aaccttttct gcaaggttct
      781 taataccttc ttcagcgttt ctcattcttt gaggtccttc aaagaagaaa ggctttgtta
      841 ctaccgcaac tgtaagtaca ccaagagatt tcgcagcctc tgcgataata ggtgcggcac
      901 ctgtacctgt tccgccgccc atacctgctg ttacaaatac catttctgta tcttttacaa
      961 gattcttgat ttcatctttt gattcttcag ccgcttgtct gccgatttca ggctttgcac
     1021 ctgctcccaa accgcttgta agctttctgc cgatattaag tacagtcggt gcttttacat
     1081 ttgcaagctg ctgtgcatct gtattgacgc aaataaattc tgcctttgta acgccggcct
     1141 cgatcatacg gtccacagca ttgttaccgc cgccgccgac acctataacc tttattcttg
     1201 cgccgtctat tgtagtgttt tcttccaaac cgataggcat atcactcatc taaatatcct
     1261 ccttcgtagt ttatccccaa caaatccaac agggaaatct ccgcatatat attattttac
     1321 tatgtttttt caacttattc aatagttttt tcccacaaat tttcaaaacg ccgaaaattt
     1381 ttacacaata ttatagtcat tttgttttta atttttgtgc aattttattt ttacggtgta
     1441 tatatcgcct ttccagactc gcttaaatcc attgttccgc gggcattttc agtcaaactg
     1501 ttactcataa cggtttctct gaacagtctt attttacgtt caagatccaa ctgcgtaccg
     1561 cattgtacgg ttattcggtt atcgtagttc attgttatgt cagtcacatc ggtaacattt
     1621 atttcgataa ccttatcaag aatacccgtt gcctcaagcg ttgacagcga aatttttgta
     1681 atatcgaatt tttcgttttc gtcggttttt aaaatttcac ccaatttata gtccgtaacc
     1741 gtcaatccag tgactgtcgg taatgtttca ccgttgttgc cggtatcggt taaaactctc
     1801 aattcagaat ttacaagcaa tgtcttaccg tcaattttta tatacgcaga cggtgtatat
     1861 tccgtcaccg taacttttac cgacggtggg aataaccttt tctgcacatc aacggtatct
     1921 atatatggta ttgtttttaa cgtattcctt atttttcgtc tgccgctgcg gaaaagattt
     1981 tgaccgacaa gcggttttag cttttcttgg aactgttcgg ctgttacgag aacatttccc
     2041 tcaaccgata cggaacgaat gttaaatatc ggtgtcataa ataagagcac gcttacaaga
     2101 accaccgcca aaacaaatcc cagcaatgca cgtctacgct gtattttaag acgctgttgc
     2161 ttttgttttt tttggtagcg aatttttcgt tcaagctgtg taggttcacg ccgaacactt
     2221 ctttcgggaa catctttatg ttctttcaag gttcttttct ctaacctgct ccaatcatat
     2281 tcagttgtag tttttgtaac cttacgaccg cctgttttga cgactcgtat attacgatta
     2341 ttttcatcca tactatcacc ctgaattatt ctcgtctgat tttcgctccg cattcaagca
     2401 gatacttttc tatattttca taacctctgt ctataaattc tgtgccgcta atcgtcgttg
     2461 taccgtttgc ggcaagtgcc gcaacaacaa gtgccgcgcc tccgcgcagt tctcttgcaa
     2521 caacatttgc accctgcaaa gcgtttacgc ctcttacaac ggcacttctg ccttctactt
     2581 ttatgtcagc acccattcgt gtaagttcat caatatgctg aaatctgttt tcaaatatat
     2641 tttctataaa tacacttgtt cctcttgcaa cagttgcaag cgccataaac ggcgattgta
     2701 tatccgtcgg aaaacccgga tacggcattg ttcttactat atgtatactt tcaagtttct
     2761 tgggagcttt ctgaataatt ttatcttttt ctattctgag ctgtactccc atatccaaaa
     2821 gcacatggag cattgctccc atatcgcgtg gattgacatt tttaagacat atctctccgc
     2881 ccgtaatcgc agatgcggca agatatgttg cggcaacaat acgatcgggc attatcgtat
     2941 actccgcacc gtgaagcttg cttactccct ctatttttat tacactgccg cccataccgc
     3001 ggacttttgc acccatacaa ttcaggaagt tacccaaatc aacaatctcc ggttcacgcg
     3061 ccgcatttgt aatagtggta aagccctccg cacaaactgc ggcaagcata gcattttctg
     3121 ttgctccgac gctcggaaaa tcaagatgta tgtttccgcc gacaatctta tcagcactgc
     3181 attttatata gccgtgttct tcctgaatat cgacaccgag ttcacgcaaa gcctttaaat
     3241 gcaaatcaat cggccttaaa ccaatcggac aaccgccggg catacttaca accgctcttt
     3301 tgcaccttga aattatcgct cccaaaaata taattgaaga acgcatctcg cgcattaaat
     3361 cttcacatat tttacagtca cacatatctc ttgtatcaac cgttacggta ttgcctgccc
     3421 tttgtactct gcaacccaat ctttcaagta ccaaatctgt tttatctaca tcacgaagat
     3481 atggacagtt tttcaaagta catactccgt cagccataac cgtagctgca agtatcggca
     3541 gtacggcatt tttcgcacct tgtacaccga tttcaccgct tatcttattt ccgccgtcta
     3601 taacaagttt gcccacttta aacacctcgc attataatat aattctcata tgattataat
     3661 acgaaatatc tataattctg ttacagtctt attattatag cacatttcaa aaaattttta
     3721 aagggttaat ttataaaaat aatataccct taatatcaat gtaacaatgc aaaatttgtc
     3781 ggttaaaaat ttcatactat ataatagata aatcaaattt ttttcttttt ttacaaaaaa
     3841 gttcttgaca atacccacac ctttatggta atatattatg cgtagcttaa atagatatgc
     3901 gtccgtagct cagttggata gagtgaacgg ctacgaaccg ttaggtcgtg ggttcgaatc
     3961 cctccgggcg taccataagt tacaatatcc gaacctcggt tcggatattt ttttgttttt
     4021 ctataaaaaa caatattttt tgcaaaaaag tattgacaaa ataattttaa tgtgctatca
     4081 tattatttgt cgtcagacat taaaactgaa tatgcgtgat tagctcagtt ggtagagcac
     4141 ctgactctta atcagggtgt ccagggttcg aacc
//
LOCUS       DAWACS010000083         4138 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2338_length_4138_cov_2.005143, whole genome shotgun sequence.
ACCESSION   DAWACS010000083 DAWACS010000000
VERSION     DAWACS010000083.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2338_length_4138_cov_2.005143"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3102..3111
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3590..3599
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1298 a          841 c          736 g         1243 t
ORIGIN      
        1 aaccatttcc caaccgaagc tgtacggttt catacccgat tcaagaagtt gtttatggat
       61 tgtgtcgtat gtttctttag gcacttttga tgtgtatgtt tgattttcga ttgtgtcgta
      121 tatcataaat tctatcactt catcctgaca ctttccgaaa cggcaaactc tctgctccgc
      181 cttttccaag tcctgcgaca ataccataag ttttataacc tccatagtct tgtcgttcat
      241 atcgttatca aagataagaa ttttttcaag caattcatta taatcggtta caaaacgtaa
      301 attatacccc tcaagtttgt caagttcacc agattctttt gacgatttct gaacattgtc
      361 aaaaagctga cttttcaaaa caacatcgtc acacggtgaa aatgaaatca taagacgctt
      421 atcttcatcg tgataaagca taggtgtagg cataagtgcc gagtacgaac aagacggaca
      481 acggaacata ttcaccttac ccgtaagcaa gtcatgcttt aaatcttcac tgtccttaac
      541 cgtaatcgag ttccatacag taacttcact catttgactg cacttagggc attttacatt
      601 ttgctttaaa ttaagactca tatttttatt cctttctaat cattttatca gcagcaacca
      661 ttattccgag ttttgcgctt tcgtatgtca aaccgccttg catatatgca atgtacggcg
      721 gttttatagg tgcgtcagcc gataattcta tcgacgcacc ctgtacaaac gcacccgccg
      781 ccataattac ctgtgacgaa tatcccggca tatcccaagg ttcaggtgta acaaagctgt
      841 ccaccggtgc acctttttgt attccctgac aaaatgctgt aactttatcg ggatcgccga
      901 attttattga ttgaattata tcgtgtctga tttcgttagg ttttgggtca acctcaaagc
      961 cgagtttctc aaataccgcc gagcaaagca gtgctgcttt taaagcctgt gacacaacgt
     1021 gcggtgccat aaacaagcct tgatacatct gtctgttaaa accgagtgac gcacctgcct
     1081 ccttgccgat tccgacagaa gtaagacggt atgcgcaaag ctctacgtac ttctgtttac
     1141 ctgcaatata tcctccggtc ggagcaagtc cgccacccgg attttttata agtgagcctg
     1201 ctataaggtc tgcaccgtat gcggtaggct cttccgtttc gacaaattca ccgtaacagt
     1261 tgtcaactat acaaatagtt tcaggcgaaa ttttctttac aaattctata agtgcgccta
     1321 tatcctttgc actgtatgtc ggacgccagc cgtacccctt tgaacgctgt attgtaacgg
     1381 cttttacatt catatgagta agagtaaatt taatctgttc aaggtcaggt gttccgtcat
     1441 gctttaaatc aatctgaaca tattttacgc cgaaatcttt aagtgagccg ttacccgcct
     1501 cgccttgtat tccgattact tcttcaagcg tgtcatacgg cttgcctgtt atcgaaacaa
     1561 gtatatcatt cggtcttaaa acagcaaaaa gtgcggttga gatggtatgc gtaccagata
     1621 tgaaattgtg acgtacaagt gcgtcctccg cacccataat atccgcatat actctgtcaa
     1681 gcgtatctct gcccaaatcg tcataaccgt agcctgtcgt agcaacaaag tgactgtcag
     1741 atacacgatt gtccgcaaat gctttcataa ctctaagctg atttatttca caaatattct
     1801 ctatatgctt aaactgctct ttgatactct cctccgcctc acttaccaat ttaagcgtct
     1861 tgtccgatat accgaaatta tcttttataa atttattctt atccataagt aaatatgtcc
     1921 tttcaaagct ttatactaac ctattaatta tatcacttat aattttttcc gtcaatcatt
     1981 atttattggt cttgtaaaaa ttgcactaat atggtataat aaaaatatct atattaagga
     2041 gttgaaatta atatgattac tataatagca aaattcaacg tacttaacgg cagtgttgac
     2101 gaattcaaaa aatgtgctgt caatgtagtg cgtgacacac gcaaggaaaa aggcaatctt
     2161 gcgtacaaga tataccaaag cagagaagat gcaacaaaat ttacattcat cgaagaatgg
     2221 cttaacgaca ctgcaataga gcagcacaac aatgcaaagc attttcttca atttcttgag
     2281 gacatcaaac cgcttacaga tggtgacgtt caaatcgaac agcttacaaa agtaccgtct
     2341 gtattctgct gattgaggga tttatggata tatacgataa taacattaat gtacttacaa
     2401 attatatagc cgatggctca aaaggctgta attcaaatgc agtcggagtt gagcttgagc
     2461 attttgtaat tgacaaaaac ggcgattgtg tgccgtatat caacggtgtt gaaaatataa
     2521 tcgaacagct tgctcaaaac ttccccaaac acgtttacag tgagggtttt cttatcggtc
     2581 tttcatgcga taaatacaac atcactcttg aaccgggcgc acaaattgaa atcagtataa
     2641 aaccgaccga aaacatttgt gagattgaaa atatttacgg cgaattttta tcggtgataa
     2701 atcccatact tgataaatat tcatacagac ttacaacact cggttatatg cctaaaaaca
     2761 aagcaaagga tatatcgctt atcccgaaaa aacgttatga atatatgaac aaatacttta
     2821 aatcggtcgg cacacgcgga ataaatatga tgcgcggtac cgcgtctgca caggtatcta
     2881 ttgatttcgc cgatgaaaaa gactgcgttc aaaaattcaa aaaagcaaac ataataagcc
     2941 ctattctttc acttatatgc gacaacgcac ctgtttttga gggcaagcat tttttgggaa
     3001 atacgttgag aacatatatt tggaatgatg ttgacaagga ccgttgcggt attgttccga
     3061 cagcgcttga cagtgatttt tcattcaaga aatatgccga annnnnnnnn natgccgaat
     3121 acatttacaa ttcaccggct atactcactc taagcggtga cgatatagaa tttaccgccg
     3181 ataaaaaaat atgcgatatt tacaaagata ctcctattaa cgaaagcata gcggagcatc
     3241 ttctttctat gttcttccct gacgtaagac ttaaaaagta tattgaaatc agacctgccg
     3301 acagtatgcc gattaagtat gttttagctt atgcaacgct tatcaaaggt atttttatgt
     3361 ctgatttttc acttgatgtt tcactaagtg ccattactca ggcaaagaac aatataatat
     3421 taaagggtta taatgccgaa gtttacggaa ccgacgcaca tacgttggct atgaaattgt
     3481 gcagtgcggc atataacgca cttgattcaa gcgacagaga atatcttctt ccgcttaaac
     3541 agcttataga gaacaaacaa acattaaagg agacgattga ccttgcaaan nnnnnnnnna
     3601 ggcagaggaa ttttataaaa aacttgtaga cgataatttc gaggaaagtc tgcaaagtgc
     3661 aaaaaagcaa caggaatata ttaaaacttc aacggcacgt tatcacggct attttgtaca
     3721 cacgctttat atgccgaaaa tgtttacgga agaaatggcg gcatacttca aaaaagccgc
     3781 cgaaagtatg tacaaaatac ttgaaaaagt tattcatgaa tatcaaacta atgccgaata
     3841 cagaaagctt ttcggttttg acgaaaaatt ggaaaagctt attcttcgtc caaatcacta
     3901 tgaatgtgcc ttgcctatcg caagaataga tatatttttt aatgaggacg atttttcgtt
     3961 taagttctgc gaatttaacg cggacggctc aagtgcaatg aatgaggata aggagcttaa
     4021 ccaagccatt cgtcttacaa gcacttttga taagtttacc gaaaaataca atgtgagaac
     4081 atatgaattg tttaattcat gggttaaagc atttatggat atttaccgta catacgac
//
LOCUS       DAWACS010000084         4131 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2341_length_4131_cov_2.927870, whole genome shotgun sequence.
ACCESSION   DAWACS010000084 DAWACS010000000
VERSION     DAWACS010000084.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2341_length_4131_cov_2.927870"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3750..3759
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1494 a          672 c          839 g         1116 t
ORIGIN      
        1 catatggacg ttacggcttt cactgattgt ggcaatcttg acaaggttca tatgtcaaac
       61 aacatcgaat gcgaaaaaga caaaaaatat tgttattggt ttccgcagac agatataaaa
      121 gaaataaaca ttcccgatac tatgcttaat tgtactaact gttattttca cggagatgaa
      181 ataacattca attcagatat gacggttaat atatacaata aagttcaaaa ttctaattgg
      241 tgtgatttaa aatatttaaa aggcaaaaca gataaaagta tgggtaacta taaaggattt
      301 gcaacagccg aaaataccat acttagatat attggaacag acaaaaatcc aatagttccc
      361 gatggaataa ccgcaatagg taaacgtgca tttacatatt gtgatattga tacggtcgaa
      421 ttaccaaatt ccttaaccca aataggagag gcagcatttt atttgtcgac attaaaagat
      481 attacaattc ctaaaaacgt caaaaaaata gaagatgatg cttttgattg gtgtccattt
      541 ttagaaaaag taacaatcaa cggaaatgcg caaataggtt caaacgcaat tttagtttct
      601 gatagactta gtgaaaataa gattgaagtc aataattcag atattaaaaa tcgtattctt
      661 tctttcggaa tttcagaata tgaattgcct gcattttatg aattgataaa ttcaaacaga
      721 gagttaatcg gttggaacaa agttgaagaa gttgagccaa ctgcaacacc ggaaagtaca
      781 acaaaaccta ctgttaccgt tccaactgca acagcactac caacaacagt gccaaccaaa
      841 gagccgactg ctacggttaa accgagcgaa acaacagcac cggctgcaaa accgaaaaag
      901 cttacggttg aaacaggcaa tgccgttacg gtaaaagtag acagcacaga cgtagtattc
      961 ccagacgcac agccgtttgt tgatgaaaac ggacgaacac aagtgccgat tcgtgcagta
     1021 tctgaaatgc ttgactgcaa ggttgattgg gacgatacat caaaaacggc aaccattaca
     1081 aaagaaaatg gcgatgttgt aaaagtgaca ctcggaagtg atgttttgac agtaaacaac
     1141 aaatcatcac aaatggatac aaccgcaata ataaaagacg accgaacatt tatccctgtg
     1201 cgtttcgtcg cagaggcact tggtttggaa gttgaatggt tggaatgatt acttattaaa
     1261 aaacggaaat atattgaacc gacacccaaa agtcagacca aaatctaaca gttgggatgt
     1321 cggttttttt ataaaaattt tttcaaacaa agacaagata tagtgcttga aattggtaaa
     1381 aatacaaaat aatctattat gaacaatcaa agaactttct gttaaaattt tttacttgaa
     1441 attataagaa aaacagtata taatataaaa tgtatagtgc gagagagtgc ctataaggga
     1501 gaggtgtcta tgtataagtt gaatgatata cataattctg atgtcggaaa caaaggcaat
     1561 tttgaaaaaa tggttatttc cgaggcggac agagttaagc ctattatcaa agaacaattt
     1621 cttagagaat acattcttgg tacgggcgat acatatgaaa tattcaatat atattgtaag
     1681 acgtttggcg tagaccgtat gcttgaaaca agaatgataa tcattaaacc ggcacagcaa
     1741 agtgaagatg aagatatatt cttcttaaaa aacacttctg agcagtacat cggtgaagat
     1801 agattattat taagcacaac aataaaaaat catttgtttc ttataacaac attattggaa
     1861 cagcaagaga ttacatcggt acttgagaaa attcataata ctatgaaaac gtgctatgga
     1921 tatgatgtta tggcggtata cagcaagatt gcgtcaatag ctgatgcacc tgcatcatac
     1981 gaacgtctga aaagatgtat gggatattcg ttttattctg acagctgcga gattttgtat
     2041 gaaaacgata ttaaaatcaa cgaaaatgct gttagtgtac aagctgaata cggcgctatt
     2101 gaacacgccg taaaaaccgg tgatatggaa aagctgaaaa tgcttttgag cggatttttt
     2161 aaaacacttg aaaaggcaat gccgactccg gcggttgcaa aaacgtattg tttagagctt
     2221 tatgtctgca taattcgttg ttgcagtgtt gaaaaaatcg aaaagtatat gaaaggaatt
     2281 gtttctgttc aagcggctaa aaagcttagt gagattaaat ctttcattga acagaacggt
     2341 tttgaaatcg caaaagccaa tgcaccggaa tctaacaaaa tatacagttc gctaattcgt
     2401 gatacgatta acataattga tgagaatatc gaaaatgaaa atctttcgct gcgatggctt
     2461 gccggaacga tattatatac gaatgtcgat tatcttggaa agctgtttaa gaaagaaaca
     2521 ggcaagaatt tttctcatta tgttatggaa aaacgtatgg aaatggcgaa agacctcata
     2581 attgagggta aaaaggacag aatttacgaa gttgccgaaa aagtcggtta cggctcaaat
     2641 tcacaatatt tcagtcaggt atttaaaaaa tataccggag tttctccgct ggagtacagg
     2701 gaatttgcaa ggttggcaag agaaaaaaat gaagcaggtt gaatttataa aaacaaccga
     2761 aaatagtctt taaattagac aattataact gatttttaac tgttaattgt attgacaatg
     2821 ctatataatt gtctgtgagt ttcgttaata gaaattggaa aaatccaatt tgtattatat
     2881 ttttaaattt tatggaggta ttcaaatggg taacaagatg aaaaaactaa tctgtgcgct
     2941 tttggcatca gcaatgcttg taggctcagt cggcgttgtc ggctttgcag atgaagatac
     3001 aaacgctgaa acagttgtaa caacttctgc agaagcaaca gaaaaacctg ccgatgcaga
     3061 ggcaacagag gctcctgccg gtgcagaggc aacagaggct cctgctgtaa cagaggctcc
     3121 tgtagctaca gttgctccta ctacatctgg tagcagctat gataacgaca gttattatga
     3181 caaggcactt tcactatgta gtgcacttgg tattatcaca ggttatgaag atggtagtgt
     3241 aaagcctgat tcaaaggtaa caagagcgga aatggcatca atcgttctaa gaatgcttga
     3301 tttaacatca acatctacat atcagaacgg ttttacagat gtaacatcat cacactgggc
     3361 tgctgatcaa attcaaacag ctttagaggc taatatcatc agcggtatgg gcgacggaac
     3421 atttgttcct gacggcgaag ttacatatgc tcaagtatgt gtaatgcttg taaatgctat
     3481 gaattatcag gatgacgctg aatattacgg cggatatcct aacggttata tcaaggttgc
     3541 aggtatgtca gaccttgaaa tcacaaagaa cgcaccgggt gcggcagacg ttgcctcaga
     3601 ccgtggcgta gttatcaaga tggtgtataa tgcacttctt ggtcaataca aggaaatcaa
     3661 cggctatgag aacggtgctc ctacttacaa agctaacgga acattggcaa aggctaagtt
     3721 tgacgttatc gataagaagg gtgttcttan nnnnnnnnnc atcaaagaca agcgtatctt
     3781 caacagacgt tcaagacggt caaatcgaaa tcgtttcaga tgaagacgat gaagctaagc
     3841 tatttgactg tgacctaaca ggtcttgaag attaccttgc tcaaaagatt acatattact
     3901 acaaggaaaa cagcggtctt acaccaaaag ttcttgcagt aacatatgac tcatcaaaga
     3961 caacaacatt caaggctgat gctgatgata ttcaaacagt tgaaggcttt gatacagatg
     4021 aaggtaaatt caaaatcgaa ggcgtaagca agaaaaagaa ctgtgccggt gcatcaatcg
     4081 tatataacgg taagattata tcaaagagcc aacttgcaga gttaggtgaa t
//
LOCUS       DAWACS010000085         4114 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2352_length_4114_cov_2.018231, whole genome shotgun sequence.
ACCESSION   DAWACS010000085 DAWACS010000000
VERSION     DAWACS010000085.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2352_length_4114_cov_2.018231"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3365..3374
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1133 a          917 c          655 g         1399 t
ORIGIN      
        1 acatattgct gttagccgtt acaacagcta ccaacgcaaa gcaagccgct gccgcagtat
       61 actgtttcca attacggcga acattacgca ttacacgtct tgttattcgt gcttttttct
      121 tttcctcttc atctatgcgg acatttaatt tctccataaa atccacagga acatttatgt
      181 cagggagaga tttagccgtt gaaataattg aacgcataaa ttcaagttca cttttgcaat
      241 ttgcacattc caatgcgtgt ttatccattt tctgctttgt ttcgtcactc aaattttcat
      301 agttgtccaa gcattcgtta aatttatcac aattcatttt tactccccct ttctttaaat
      361 tttcattaat ttctttttac aatatattag acgtaagaaa tatcaaaaaa gttcccgttt
      421 attcattaaa tttttcttta atgcacttct cgcacgatta attcgcgact taaccgttcc
      481 cgacggtatt ccgagtacct cagctatgta atcgtaactc aatccctcaa tatcgcatag
      541 tgttattaca gttctgtatt cttctttgag ttcgcttatc gcctctcgta cagctctctg
      601 acgttccgat atttccatat tttcttccgg tgtaggactt tcgtccctaa tatccatatc
      661 tttcttttcg tcaatcgcct gattaatact tattacaccc gaatgtttct gacgttttct
      721 cagaatatcg gagcaaacat tcgtagttat cctataaagc cacgtggtaa aggaagattg
      781 ctccttaaat gactctatac ttttatacac ccttacgaat acctcctgtg ccgcgtcata
      841 tgcgtcctct tgatttgaaa gctttccgta agcgatattt ataacttgct gttggtaatt
      901 tgaaactaat atattaaaag cttctcggct acctttttta cactttttaa tcaaatcatt
      961 ttcggtcaaa aatccctcac cacctctcat ggtttatttg ccctacgttt gattttagaa
     1021 attcttaatt ctgttcatat atacatcgta atatgccttt acgtcagcca tacagtcacc
     1081 gctgaattta tccaaaaacg cctttgcgat ttcaaatgcg attaccgcct caacgacaac
     1141 cgaacaagcc ggtacggcac acacatcgga acgttcgaca gttgccttat gctccgattt
     1201 atcgtcaata ttgactgttc taagcggatt atacaaagta ggtatcggtt tcattgccgc
     1261 cttaacaata atcggctcac cgtttgacat accgccctca ataccgccgg cattgttggt
     1321 aagtctgtgg aagtcgtccg aatattcgat ttcatcatgt acctttgaac ccggtatttc
     1381 gctcacaccg aagcccatac cgatttcaac gcccttgata gcctgtacac tcataacacc
     1441 gaaagcaagg tttgcatcaa gttttctgtc gtaatgaaca taacttccca atccggcagg
     1501 cacacctttt acgatagttt ccactattcc gcctacgctt tcaccgcttg ccttgatttt
     1561 gtctatgtat tccatagcct tttgagcggc attttcatcg ccaaacccaa caggtgaatt
     1621 ttttactttt tcaaagaaat ctgccgcagt aaaatcagct gtatcttcaa cttcgcctat
     1681 tctcttaaca tggcttaaaa attctattcc aaacggtttt aaaacctgtc ttacgagtgc
     1741 gccgacagcc actcttgctg cagtttcacg ggcacttgct ctttcaagca cgtttctcaa
     1801 atctctgtga ttgtacttca tacctccggt aaggtctgcg tgtcccggtc ttgggcattc
     1861 gacagacttt tcgttattcg ccgtttcagt acccattata ctttcccaat tcgcccaatc
     1921 tctgttttcg attttaattg cgataggact tccgagagtc actccgcctc tcacacccga
     1981 aagaatttcg gcagtatctt tttcaatctt cattcgtccg cctctgccat agccctgctg
     2041 acgctttgca agctgttcgt ttatctcatc aaggcttatc tcaacacctg acggcatacc
     2101 ttcgattata actgtaagac acttaccgtg tgtctctccc gctgtgaaat atctaagcat
     2161 atttttatct ccattcgtaa ctacaatttg tattttttta atatataaac tacaacataa
     2221 tgtatataat ctattttatc actttttcgt tacttttgca agttttaacg acaattttac
     2281 aaaactcgct ttgtgtaatt gtaataaaaa agatatattt aaaataggaa tattatgata
     2341 taatttgaat atcactaaag tcactgagct atttcataca gaaaggaaag gtaattttta
     2401 tgaaaactac aatctgggga aatattcttc ctgccgtaac aatcgacctt gaacaaggcg
     2461 aaagcattta taccgaatcg ggcggtatga catggatgtc ggacaaagta aaaatggaca
     2521 ccaatatgaa aggcggattt atgaaaggtc ttggacgtat gttcagcggt gagtcattgt
     2581 ttatggctac atacaccgcc gaggcaccaa accaatctat cacaatcgcc tcatcttttc
     2641 ccggagaaat caaagaactt cacatttctt ccgacaaaga atttatttgt caaaaatcag
     2701 cttttttgtg tgccgagcaa agtgttgaac tttcaacata tataacaagc ggcataaaga
     2761 aaggcttgtt cggcggtgaa ggctttgtac ttcaaaagct ttccggaaac ggtactgctt
     2821 tcattgagct tgacggttca gtaaaagaaa tcgaccttgc tgcaggcgaa aaacttaaag
     2881 tcgatacagg cagtgttgcg atttttgaat caaccgtcaa atatgacgtt gaaacagtaa
     2941 agggctttaa aaatatgttg ttcggcggtg aaggactgtt ccttacaacg cttaccggtc
     3001 cgggtaaggt atatttgcaa acaatgaata tggctgaatt tgcaaataaa attatcccgt
     3061 atcttccgac accgtcatca aaatagcaaa taggaattat aactaaagaa gtggcaaaaa
     3121 tattttgcca cttcttattt tttatctggt taaataatta taaatcataa ttatactttc
     3181 cgctcttgtc atttcacggt ttggattgaa attaccgtta ccgtcaccgc ttaacactcc
     3241 catagccgaa agcaatgcga tatatccctt attttccgta acatcattga acggtgtaac
     3301 gtaaatatcg ttatatttcg cgtaactttc agcaccgatt gcacgaatca tatacacagc
     3361 agccnnnnnn nnnngcttaa attcaccgcc ctcaaagcct attccgtaat aagcaagctc
     3421 tttgatatac ttttcggcat agtgaccgtc tatatccgaa tattcaagtt tactgcttgc
     3481 atcctccgta atttctttgt tttgatagtc aacaaactta cctgtaaacg gattgatatt
     3541 ttctgtatct attgtgtata cagggataat ctctcttgtg ttgtcagtat aattctgcat
     3601 atatacaatg ctgtaatcct ttacgtcaaa caaaatattt tcagcatctt ccttgctcat
     3661 tgcattttcg attgacggaa attcggtgtc ggtataattc atactgaaat cagtaagtac
     3721 gccgttgttt gttgtcacga tatactgacg tgaattttca tcatatttgt attcttatgc
     3781 cttatcacct gcaagtgtct ttaaagccga atcatcatta ttttttacct cactgtcatc
     3841 ttctccccaa cgtgaataat actttatttc acctgtttta gcatcaacag aagtatatat
     3901 gttgcacttg tcatttgaca ttgatacagc atatgtatat tcatcgtcat aatttttata
     3961 taaagaaatg ctgtctgtat ttatattctt agggattgac aaaagcttgt tcgactttaa
     4021 tttattttcc aaagcggttc ttgaaataag tccgtctata ttgtcaagct cttttatttc
     4081 ctgttcggta agtccactgt ctgctgtatc accc
//
LOCUS       DAWACS010000086         4045 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2413_length_4045_cov_2.186216, whole genome shotgun sequence.
ACCESSION   DAWACS010000086 DAWACS010000000
VERSION     DAWACS010000086.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2413_length_4045_cov_2.186216"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1374 a          671 c          981 g         1019 t
ORIGIN      
        1 ctgttaatat gggtattaac tcaaaagaga caggtgaatc tgcatcatac tacacattgc
       61 ttaataacta ctatgtagaa ggtattattc aacgtggtgc tattcctgta attgttacag
      121 ctacaccgct tggattcagt agttcacagt atccgtataa tgcatcaaca ggtaagttta
      181 cagtaaatcg tggtacaggt gctcataacg gtgatttgag aaaaattgct caatcacata
      241 atttgaacat tattgaattg ggttactatt ttgaagatta ctttaatagt ctaacagctg
      301 aagatgttgc agcttataac gctgaaaacg gaacaagctt tacatcacaa gttgaacttg
      361 taaagagctg gtatggtgac cacaaccact ataaacagta tcttgctgat aagattggtt
      421 catatatcct tgacagtgtg tcgaagattg caggcggaag tacaaacttc aaccaggcaa
      481 atgatacaca catcaatgaa caataatcaa cttaaaagaa cttcccgaac gggaagttct
      541 ttttttgtgc ctgcttccat atacataaac taaaaagggg atttgggtgt gaaaaaaata
      601 cttttagtag ttgttaaaat catttttatt acgttgcttt tgccgcttgc gatagtttgg
      661 ttaatcggtg gcagtggaga aggacaaagt ccgattgaga gcggaacggt aagtgtgtat
      721 gtgtcagaag aagattgtgt taaagatatg gatattaatg aatatttaaa atgcgtggtg
      781 gcggcggaaa tgcctgccga ttttgaggaa gaggcattaa aggcacaagc cgttgcggca
      841 agaacatatc tgtactcaca tattgccgaa aaagacaaag gcaacattgc cgaaagacac
      901 aacggtgcgg tgatatgcac cgattcaacg cactgtcagg cgtatatttc ggaggataag
      961 cggagagaaa gctggggtgc cggtgccgat gaaaattgga acaaaataag cacagcggtt
     1021 gacgaaacta caggacagat aatgatatac aacgatgaaa taatatcggc ggtatttcat
     1081 tcaacttcgt cgggtatgac ggaatcggca gttgacgttt ggggggcgga cgtgccgtat
     1141 ttgcaaagtg ttgcaagtat gggagataaa gaatcaccga agtatcacag tgaattgaca
     1201 ttgagtgaac aggaatttaa aaatattgcg gaagaaaaaa ttgacggtgt ggattggaat
     1261 aatggacttg tatcgaacat taaccgctca aatgcaggcg gtattgtaac acttgatgta
     1321 ggcggagtga atataaaagg aacagaattt agaaatatat tttcactgcg ttccgcaaat
     1381 gttgaaatat cgcaagaaaa cggcaatata aaaatgagcg taaaagggtt cggtcacggc
     1441 gttggtatga gccagtatgg tgcagattac cttgcgaggc agggaaagac atataaggaa
     1501 attttaaaaa catattatac aggtgttgaa atagaaaata ggtgaaattg cacaaaaaat
     1561 gatacgaaaa tttgtgactt aaaaaaaaat atgactgttt tttatattga gaaattccgt
     1621 aaaaagttat aaaatattat tataattatt tttgagggag agagttaata atgaacagaa
     1681 aagtgatcag tgcggcggtt gcagttgcaa tgaccgccac aatgggcagt tttgccttac
     1741 cggcaaacgc cgcggaagtt aaaactccgc aatatcaaac aaacacacgt caaatggaaa
     1801 agcttaatcg tggattgatt gctgttaaga caacggcaga tacacgaggt caggcagtta
     1861 acggtgtata tttgagttgg agattgcttg gtgacgaaag ccttgaaaat caagcattcg
     1921 atatttataa aaacggtaca aaaatacata caacaggtgt acatgacgct acaaactgga
     1981 ttgatacatc gggtactgca tcggataaat acaaggttgt aaaagcgggt gaggacgcgt
     2041 ctaaggaaac tgaagttaca cctacttcaa acaacaactg tgcaaagtca aatgaagtcg
     2101 gaaatggaaa cagtgagaaa aattcattta catatgtaga tattcctatt tcacgtcctg
     2161 atccggtaga aagaatgggt gacggcaaga tttcaaatta ttacactgtt gacaaaagcc
     2221 acgaaggcgg tgcgaatgac gcgtctgtcg gcgatcttga cggtgacggc aattatgaaa
     2281 ttgtattaaa gtgggacccg acagactcaa aagacagtgc cggtgcggat tttacaggta
     2341 atgcatatat tgacgcatat aagattgatc cgaacaatga cggttatatg tggcgtattg
     2401 atttgggtaa aaatgttaca tcaggtgcac actatacgca atttttggtg tatgactttg
     2461 acggtgacgg aaagtcagaa gtggcaatga aaacagcacc cggtacggtt gatggcacag
     2521 gtcattacgt tacggaagtc ggtgatactg acgaaatcag aaataccgac aatacaaaat
     2581 catatatagg cacaagcgga agattaaagg gtaaaaatcc gtttacgcag tatttaacca
     2641 tatttgacgg cgaaacaggt gccgcacttt atacaacgga ctatattccg tatgatgcgg
     2701 cagaagatca atattggggc gacggcaagg caaagtataa cagaagtgaa agataccttg
     2761 cggcagtggc ttatcttgac ggtattcatc cgagtatcgt tatgtgcaga ggttattatc
     2821 acgactctgt aattcgtgca tatacatggg acggtactga acttacaatg cagtgggagc
     2881 ataaaggtaa gaagagtgca agttcaacga cactgtacgg acaaggaaat cataatcttt
     2941 ctgtaggcga tattgacaat gacggcaagg acgaaattgt ttacggaagt gccgcacttg
     3001 atgatgacgg taaaacagtt ctcggaaata caggtcttgg acacggtgac gcaatgcata
     3061 tgagcgactt taataatgac ggcactcaag aagtattttc ggtaaaagag gaggaattta
     3121 aaaagtatgc cgaagattta agagtcgcaa gcacaggaaa gcatttttgg agtcccggaa
     3181 aactgactac atcaagtgat aacggacgtg gtgttatgga taacattgat gatagctacg
     3241 caaaggaaca ttcaaatgca ttggcaatag gttggagcag cggaattgcg aatgctcacg
     3301 acttaaacgg tgatgatgtt gccgcaaaac cggctggtgc gggaagcggt acctttgata
     3361 atttccttgt atattgggac ggcgatttga gccgtgagtt cacttgttgc cgatatttgg
     3421 ggcgactggc gtgaagaaat tataatgccg gtaaacaaag gctcggcaac ggatcaggca
     3481 tatttgagaa tatatacctc tacaatgcct acagattaca gaattacaac attaatgcac
     3541 gactgtcaat acagattgtc agttgcgtgg cagaatgtcg gatataacca accgacacac
     3601 gcaagctatt atataggttc tgtggcactt gcaacagacg aaagcggcaa tacacttaac
     3661 tatcttgcac cggcagtgcc ttatacaaag gtgacatatt cggcaccgga gcaagtagcg
     3721 gttacgggaa tgactttgga gaaaaagtca atagaagttg aaaaaggcaa gacagaaaca
     3781 atcaatgcga ttatcacacc tgaaaatgcg acaagaaagg gtataacttg gacttcgtcc
     3841 aatacaaatg tggcaactgt tacaaacggt gttgtgaaag gtatatcagc cggaactgca
     3901 actataacgg caacaaccaa agacggtggc tttacagata cttgtgaagt gactgttacg
     3961 caaaatgcag ttacgggcgt aagaatttca gagaaactaa ttgatttggg aatgggttac
     4021 aaaaagcaaa ttaccgcaac ggtaa
//
LOCUS       DAWACS010000087         4042 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2418_length_4042_cov_2.584399, whole genome shotgun sequence.
ACCESSION   DAWACS010000087 DAWACS010000000
VERSION     DAWACS010000087.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2418_length_4042_cov_2.584399"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1157 a          710 c          876 g         1299 t
ORIGIN      
        1 atttaattct aatcagtaaa tatattataa aaaaagtggc agaaaaactg ccactttttt
       61 tataatgatg tttagttatc tgcaagcatt ctgtttactg ccgaaataag tgcatagatt
      121 gacgcggtgt taatatttgc gtcaacgcct gcaccccaat aagttttgtg gtggttgtgg
      181 tcgttgattt caatatatgt tgccgctctt gaatttgatg aacgtgttag ggcgtgttct
      241 gtgtagttgc agatttcaaa ctgctgaccc aagaattgtc ttagaccgtt gcaaagtgcg
      301 tccaaaggac cgttaccctt gccctcgata gaggtaactt ttccgccctt ttcgatagta
      361 gccgaaagaa ttgtacagtc atcttcctcc gttgaacgat agaaacgggt tttaataggc
      421 tctgccacat tcacatatgt atccttgaat gcttgatgta tttcgtgatt tgtaagtacg
      481 gttttctttt cgtcagacat atctgtgata agtgaagaaa attccgcaag cattggcttt
      541 ggtagggtgt agccgtactt gttttccata atatagctta caccgccttt acctgactga
      601 ctgttgataa gaataggctc atattttctg ccgatgtcgg tagggtcgat tgtaaggtat
      661 gggttagccc atttatttga cggacttgaa ccgaatttat ccatactctt tttgattgcg
      721 tcctggtgag aacccgaaaa tgcaacgtat gccatttcac ctgcgtatgg gtgtcttggg
      781 ttaatcggca aacgattata cttttcgtat acttcgatga tttcatcaat attttcaaga
      841 tgaagttcag ggtcgatacc ttgacagaac atattaagtg caacggtgat aacgtccgtg
      901 ttacctgttc tttcaccgtt accgaatatt gtaccctcaa ctctgtccgc acctgcaagc
      961 aatgcaagtt cgcttgacgc aacgcctgta cctctgtcgt tatgtgcgtg aacgctgata
     1021 acaagatgtt cacgattgtt aaggtgcttg cacatatatt cgatttggtc ggcatatacg
     1081 tttgctgttg caacttcgat agttgacgga agatttacga taactttgtt tgtttcggac
     1141 ggcttgaata catcaagtac ggcatttgta acttctgccg cgtagtccgg ctctgtacaa
     1201 gtgaaacttt caggtgaata ttcaaaacga atatttccgt caagctgacc gtcaacaagg
     1261 ctcattacaa gctttgcacc gtcaacggca agttgtttga tttcttcctt gctctttctg
     1321 aaaacaactt cacgttgaag tgttgaagtt gaattgtaaa ggtggataat tgcgttttta
     1381 gcacccttca gagcctcaac ggttttcttt ataatatgtt ctcttgcctg tgtaagcact
     1441 tgaattgtga catcatcggg aataagattt tcgtcaataa gacggcgaac aaattcaaat
     1501 tcagtatgcg acgctgccgg aaaacctact tcgatttctt taaaaccgag ctttacaagg
     1561 aatttgaaaa attcgatttt ttcttctgtc gtcataggtg agtaaagtgc ctgattaccg
     1621 tcacgaaggt caacactgca ccaaatcgga gcatgagtta tttcattgtt cggccactga
     1681 cgatcctcaa gcggaatcgg atggtacatt ttttgatatt ttttataatt cataatgaac
     1741 actgcctttc cggcgaatac gcctaaaata atatatgaaa tttctaacta aaaaagctga
     1801 gccacaatgt gacccagcat aatttcaata cagttcagta cagaaaaaca aaactgaaga
     1861 ccacattgtc gattcagctt aggagtagga ggttattcaa atttgccttt gaaaatttat
     1921 ccataattcc gtacctcttt ctatactata tggctattct acaataaaat caaggctttg
     1981 tcaatatttt ttcgaaaatt aatatataga atttataggg tgaaattgcg gattatatag
     2041 tgatttttta tacattaaag gaatagacgg agcatatatt ggacgtagga ggattgattt
     2101 atggatatac tgtctttatt gctccttgct gctgcacttg cgatggacgc attttcggcg
     2161 gcggttacgg acggaatgat tgtgaaaaaa atacgatttt atgacgcgct taaagtggga
     2221 ttgtttttcg gtgtgtttca gtttctgatg ccttgtatcg gaaatttggt gtcgggggtt
     2281 gcgactgata ttgtggaaca ttttgaccat tggattgcgt ttgtgctttt gggatttttg
     2341 ggactgcata tgatatggga gtcacgcacc gaacaggaaa taccgaaaaa tcctttaaaa
     2401 aattcatcgc ttttgcttgc ggcaatcgca acaagtattg atgcacttgc ggcgggggtt
     2461 acgcttgcgg cggttgatac accgatttta ttttcgtccg cggttatcgg aattacagca
     2521 tttttgtttt cgttcggcgg tgtttttatc ggtaagaagt tcggaggttt tctcggctca
     2581 aaggcagaat gtatcggcgg aattattctt atattaatag gcgtaaaaac acttgtcgag
     2641 catttatatt gactaaattt gcaaagtggg atataatgat attcagagct gtcgaaattt
     2701 tgtcggcagg ttcaatgata tgtacttaat atgtatgaag ggatattata atatggattt
     2761 aacttcacca cgcacgataa gaaatattca ggaacaattc ggttttacgt ttaaaaaagg
     2821 tcttggacag aatttcctga ctgcacgaaa catacttgaa gaaatagttg acgccgcaga
     2881 gattgaaagc ggtgttatag aggtcggacc gggattcggt gtgcttacga gcgagcttgc
     2941 gcaaaacagc gataaggtcg ttacgataga aatagacgaa cgtttgattg atgtgcttga
     3001 ctacacactt gcggaatatg acaatgtaaa aatcataaac gaagatattt taaaacttga
     3061 tttaaaaaaa gttattgacg atgaatttaa cggcgaaaaa gtcagtatag cggcaaattt
     3121 gccgtattac atcactacac cgattatcac taaattgctt gaggaaaaat taccgattaa
     3181 aaatatagtg gttatggtgc agaaagaggt tgccgagcgt atgtcggcaa agccgtcaac
     3241 aaaggattac ggtgcgataa cggtgctttg tcagtattat acggagccgt caatcgtggc
     3301 aaatgttcct gcatcactgt tcgttccgcc gccaaaggtg gacagtgcgg tgttaaaatt
     3361 aaaggtactt gataaaccgt cggtggaagt aaaggacgag ggtgtatttt tcagagtggt
     3421 gaaagcggca ttttcacagc gaagaaaaac tcttttgaat tgcctttcgg caaattttcc
     3481 tattccgaaa gatgaaatat caacacttct tgaaagtata ggaattgcac cgtcaagacg
     3541 cggtgaaaca ctttcgctta aagaatttgc aaatattgcc gatacattat tacaaaagat
     3601 aaaataggag gattttcaat ggaaatcttt tcatttggtg aacgacttat gatttaccgt
     3661 aaaagagcgg gattttccaa aaaagaactt gctaaaagag tagggataag tgtttcgaca
     3721 cttaccaagt atgaaaatga cgaatcacaa ccgaatgttg atatgcttac cgatttggct
     3781 attgcacttg acatatctgt tatgaaattg ctttcaggtt tcggtgatcc gattcgtgaa
     3841 aaatacagac agggactttt aaatatttcg cttgaaaaaa tgaatgctct gcaagaaagg
     3901 catttgctat aaaaaaatag gattgcgatt attgcaatcc tatttttatg tcatgttttt
     3961 tatttcttct tgttgcctgc ttttgctcga aggcttgtgc ttagaatttt ctttctcatt
     4021 ctaaggtggt ttggagttac tt
//
LOCUS       DAWACS010000088         4032 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2428_length_4032_cov_1.825497, whole genome shotgun sequence.
ACCESSION   DAWACS010000088 DAWACS010000000
VERSION     DAWACS010000088.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2428_length_4032_cov_1.825497"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1819..1828
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3245..3307
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3653..3662
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1163 a          752 c          761 g         1273 t
ORIGIN      
        1 gttgaaatca agacagtccc tgttgccaat gttgaaaaag gcaaatattt ggaaagtgaa
       61 attacttttg acaaaagtat caaagaaaat aatttgaaag tatttatgtg gaattcgctt
      121 ggcagtatga cagcactttg cgaaaatgct caataacaaa tttaaaaaca aaaatcgcag
      181 actataaggt ctgcgatttt tttaatgtgt tattcagtat gttttcgagt tctattgctt
      241 ttgatacgac ttcggggtcg gaaagacaat aatttttttc aattataaga ttatcaaaat
      301 ctgttttcag ttgtttaatg ttgttcattt atgtatgctc ctttcgatta cacggttata
      361 tgtaaaccac tgtttgttgt atttatttta caatatatga taaaacttgt caatatattg
      421 tggtgtaaaa ggagcaactt tcgtgttgca gaaaatgaca caatcgttta catttcgctc
      481 tctttcttcg ctttaacctg taaaacgaca aaagctgaca cagcggcaat cataaacagt
      541 attgcaacaa gctgagaaat gccgagaaca tttggaatta catataatat ataattcgga
      601 tttctcatac cttcaagaaa cagtctgccg aatgaatacc agaaaatata gaaacaaaat
      661 acttgtccgt tattcttttt cttgtcacga atcagaagaa gtatcacaag tccgataaca
      721 ttccatatcg attcatataa aaataatggg tcaacaggtg ttgcgccgtt tatggacata
      781 ccgaaaaggc tgtttgtttc accgccgtac acttcaacgt ttacaaagtt tccgaaacgt
      841 ccgattgctt gaccgataaa aagaccgggt gcacatacgt caaagacttt ggcgatattc
      901 agctttttaa ctttgcagta tataacggtt gagattatcg caccgataat accgccgtaa
      961 atagcaagtc cgccgttcca gactttaaac atatcggcta acgagtcgaa ttcatcgagt
     1021 gcgaaaagca cataatatat tcttgcgccg attattccga aaataagtcc gagcaaggcg
     1081 atatcccata cgttatcttt tttcacacca cgcttttcgc aggttttata aacgaaaaac
     1141 aatcccaaaa gaaaacccga aagaatgata agcgcatacc aataaatagg ctttgaacct
     1201 attgaaaatg cgacaggtga aatgtttaga gaaataccga gtttaggaaa agcaatgttg
     1261 ttcataatca tacctccttc atagtaagtg aaatacgttt tttcggtacg tcaacttcaa
     1321 gtacacgaac tttcacaacg tcaccgactg aaagtacgtc ggttgggtgc ttgatgtatc
     1381 ggtcgcatat ttgcgatatg tgtacaagtc cgtcttgatg aacacctata tcgacaaatg
     1441 caccgaaatc tataacgttt ctcaccgttc ccgtcagtac catatccggt tttaagtcct
     1501 ccattgagag aatgtcgctt cttaaaacag gttgcggcaa ttcttcacgc gggtcgcgac
     1561 ctttttttat aatactgtcg gcaatgtctt ttaaagtgat ttcacctatt ccgagttctt
     1621 cggctaattt atgtgtattt tctttcgaca gacgttgctt taaatccgca acagtgccgt
     1681 cttgaacgtc ttgttcggta tatcccatat gtttaagcag ctgcgcggcg gctttgtagc
     1741 tttcagggtg gacggaggtg ttgtcaagga cgttgtcacc gtctgttata cgcaagaaac
     1801 ctgcgcactg ctcaaatgnn nnnnnnnnta tgtgattatg ttttttgaaa cggttttgtt
     1861 aattccggca acatatgaaa gcagtgacgg agaggcggtg ttaaggtcaa caccgacact
     1921 gtttacacaa ttttcaacca ctcctccgag tgcctcacca agtcgctttt ggttcatatc
     1981 gtgctgatac tgtccgacac cgattgactt agggtcaatt tttacaagct ctgcaagcgg
     2041 gtcttgcaca cgtcttgcta ttgaaacggc acttctgaga gcaacgtcat actgcggaaa
     2101 ttcttcggtt gcaagctttg acgcactgta aaccgacgca cctgcctcgt ttgtcataat
     2161 gtaaaatact tctcggtcaa tttcttttaa aaggtcggaa ataaatattt cgctttcctt
     2221 tgaggctgtt ccgttaccga ttgaaattaa gtttacgttg tttcttaaaa tcatatccgt
     2281 aagaattttc ttagccttgt ctttgtcgtg gttaggtaat gtgcagtaaa caacattggt
     2341 gtcaaggact ttgcctgttt cgtccacaac cgcgactttg caccctgttc tgtaacccgg
     2401 gtcaaagccc ataacgattt tatccctaat aggcggttgc aataaaagtc cggaaagatt
     2461 ttgattgaat acctttattg atgcctcctc ggcattttcc gtaagcatat tgcgtatttc
     2521 tgttgatatg gacggtgcaa taagtagttt ataactgtcc tcggcggcaa gtttgacgta
     2581 ttttgtaggc ggtgattcgt gttttggagg aagtcgtctg aaaagatagt ttgtgatttt
     2641 ttcttcgtca atatcaattt ttaccgataa aaatccttct ttttcgccac gatttatcgc
     2701 aagcaatctg tggtttgcaa gtttttttat tggttcggca aaatcatagt aaagacggta
     2761 tacactgtcg tcttctgttg acgcttttga aaccaaaaga ccaaagtcga ttgttttttg
     2821 acgaatatct tttctgtatt cggggttatc actgatttct tcggcgatta tatccatagc
     2881 accggctatt gcatcttcac tgctttcaac atctttttcg gtgtcgataa aatcaagcga
     2941 aatactttct atattatcat tcggattttg tgcaaggata atttcggcaa gcggtgccag
     3001 tcctttctct tttgcgatag tcgctcttgt gcggcgtttt ggtctgaacg gacggtatat
     3061 atcctcaagt tccgataatg ttacggcatt ttcgattgat tttgcgattt catcggtcaa
     3121 attttcttgc tcgtcaatca aacgcaatat ttcttctttt ttatcttcaa ggttacgtaa
     3181 atatgacagc ctgtcgtaaa aatctcgtaa tatttcatca cttagagaac ctgttgcttc
     3241 ttttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnncca ttatgtatat cctccaaaaa ataatgtttt gtcttgaaat tgttctgtaa
     3361 caattctgca aatgaatttt aaaaattcta ttcacaaact gaaatatttg tgatataata
     3421 gtaacaacca caatatatag tgtgcaatta tgtaaactca acaccaaata tatatgtgat
     3481 taaaaatgca gttaagctaa ttatatccca tttagcttaa ttttacaagc aaaacagtaa
     3541 aatgagttaa aatgagatgt acaagctgaa aaaaatacaa aaaacctatt gcacttttga
     3601 ttgatttaag gtaaaattga gttaatggga gcgaattgga gcgtttgggg tgnnnnnnnn
     3661 nnaggcaggt gacattgaat atggtaaatt ttgttgacga atatccgcgt caacttgacg
     3721 agcgcggaag aattatcttg cctgccaaag ttagagaaaa tatgtccgaa acagtgtacg
     3781 ttacccgttc aatgtcagat aaatgccttc aattatacac acaagaggaa tgggataaga
     3841 tttctgaaaa gattaatcaa ctcccaactg ccacagacag aaatgctgcg gcttttgtac
     3901 gtttgttttt cggtaaggct acttcagccg ttatagacaa gcaaggcaga gtgccaatcg
     3961 caaagcgtct gatggaatat gctaacctga aaaaagatgt tgtgcttgta ggtgcaaaca
     4021 cacgtcttga aa
//
LOCUS       DAWACS010000089         4023 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2434_length_4023_cov_2.136593, whole genome shotgun sequence.
ACCESSION   DAWACS010000089 DAWACS010000000
VERSION     DAWACS010000089.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2434_length_4023_cov_2.136593"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1299 a          611 c          874 g         1239 t
ORIGIN      
        1 cttatcagca cttcacattc aatgcgttga taaagataag atcatactta atccggcttt
       61 aatcaaaaaa taatttaata agaaatttaa gtgcggtatt tgaataccgc actgtttttt
      121 tgcataaatt ttcttagggg gtgaggctat gaggaatata cggaatattt taagcgtcgt
      181 atgcggttat gtggcggcgg ttatcggtgc gggttttgcg tcgggacagg agattataag
      241 cttttttgtg aaatacggaa aatacagcat tataggtgta ttgctttcgt gcgttatttt
      301 ttcggtgttt gcgtatgcgg ttttatcggt atgtgtagaa aaaaatatag aaacttattc
      361 ggattatctg aacaattttt ttagatataa tataagaaaa attgtcgaaa ttataacttt
      421 actttttgca atatccacag tatgcgttat gacggcgtgt gcaggggaaa tgttttttat
      481 attgttcgga attaaaaaaa tattcggcgc aattattttt aatgcagtat gcggaatgat
      541 attttttatg aacaataaaa aaattatggg gataaattcg attttgggtg caataataat
      601 tttcggtatc attttttgtt gtttctatat attgagattt cgagaacatc aagttttttc
      661 aaatgaagtt aaaatgactg tttcaagtat ttcatatgcc ggatacaatc ttataacaac
      721 aggtgcaatt cttgcgggta tgagccgttt tttgcaagac aggaaagagg cggcattggc
      781 aagcgttatg tccggagtgg tgctgtttgt tatgatgtta cttatatgga ctgtgcttga
      841 aatatattac ggcaaaataa atctcggaga aatacctatg ctcacaatgg ctctcagaca
      901 aaacaaagtg tttggagcga tttacggaac gatgcttttc tttgcggtca ttaccacaga
      961 cgtatcaaac ggatttaacg tacttgacat agcaagcaga aaaataggga agaatgttgt
     1021 tgtattcttt atgactgtaa ttgcggttgc tatgagcggt gcgggttttt ctaaaattat
     1081 aaatacagct tacagacttt gcggttacgg cggattggtg cttgttttct atgttattta
     1141 taattttata aaaaatatga ataaagtaga aaatgaacgt aaaaatgaga atacaaaagt
     1201 aaaataagca aaaaaggaat ataatcgact gtaatcgagg tttacaaaac taaatatctt
     1261 tgctattata tacaagtcgt ttggcgagag caaaacggca gagaacatac gtcgggatgt
     1321 agcgcagttt ggtagcgcat ctggtttggg accagagggc cgcaggttcg aatcctgtca
     1381 tcccgaccaa aaagtatggg agtttagctc agctgggaga gcgtctgcct tacaagcagg
     1441 atgtcacagg ttcgagccct gtaactccca ccacttttta aataatgttc ttaaatatga
     1501 tgctggtgta gctcaattgg tagagcagct gatttgtaat cagcaggtcg cgggttcgag
     1561 tcccatcacc agctccataa aaagggataa actttcgggt ttatccctca tagtttggga
     1621 gagttcccga gcggccaaag ggggcagact gtaaatctgt tgtcttcgac ttcgatggtt
     1681 cgaatccatc ctctcccacc acatcagagc aagctgtata cagcttgctc tgattttttg
     1741 tataatattg ttttgaacgt ttttgaacgg aatatttgat atttttcttt gatttggttt
     1801 cgcaaatttg gtgtattttt gaatgcaaaa taaaacaaaa ttttttgtaa caatttgtaa
     1861 caacttctta aatcatttaa acaattttgt aagtctgttg ttgttgtaat aaatattcat
     1921 acggcgtata atagacttat aaagagaaca ggaaatgaat gaaagggagt gtcaaatatg
     1981 aagaagaaaa tgttagttgc ggcgcttatg cttatagtta tgatgactca aatcagtgca
     2041 ttcgcttcat catatgcgac atatgccgtt cataatgaga aagctgttct gaaagctgcg
     2101 gataatctcg gcgaatatgc ggtaatacca aaggaacttg aaggtgtgac ggtagaggga
     2161 atcggagcgg atgcctttaa aaacaacaaa gagttgaaag gtatagaaat tcctgaaaag
     2221 gtgtcatata tcgaatgggg agcatttgaa ggttgcgata atcttactga tataaatata
     2281 cctcaaaatg tgatgaagat tgaagatatg acatttgctg actgcacaag ccttggaaac
     2341 atcaagttac ccgaaaaatt acaggaaatc ggagtgaaag cattttcaaa cacagattta
     2401 aaggaaatcg taattcctga cggcacgaaa gccatagata taaaagcatt tgaaaattgc
     2461 aaaaatctta aaaccgtagt tttgccaaag agcgttgaat acattgctgt cggagcgttt
     2521 gacggctgtg aaaaagtcaa cgtgaaatgt gtaaaaggca catatgccga agaatatctg
     2581 aaagctaaca aaatttcata tattgcacat tagtataaaa atttaatatg ggttaataat
     2641 taaagggtgg tatggaaata tcaccctttt tgtgtgctga tgaattatct gaaatgcaat
     2701 ttgacttttg gatatgtatg taaagtttac aaatagttta aaaacataaa gtgttttttc
     2761 tattgacgag agtaagtttg aatggtataa ttaaaatata ttgtattttt ttgaaacata
     2821 gatgaatatt tttggaggtt acagagtgaa agttaaattt aatgtaaaaa gatattttca
     2881 agtgcttgga atatcactcg cagtgattat tgccgcggca gctgtctgta tgggaattga
     2941 cttttcgggc ttaaacaatg aggaagcggt ggacaacact tcgactgttg aggcggcaga
     3001 cggaaagata aacgttttac ttatgggtgt tgacgttgac ggcttgagaa cagacgcaat
     3061 aatgcttgca agttttgata ccgaaacaaa agaggtgaat atgctctcaa ttcctcgtga
     3121 tacaaaaatg tatataggaa accgttatca aaaaattaac gccgcacacg catttgtgga
     3181 tgaaagcgga gaaatcggcg gtgcgacagc aacttgtgag gcggtaacga gaataacggg
     3241 tattccgata aattactatg ttgatttcag ctttgacgct gttgctcacg ttatagacga
     3301 acttggtcct attgaattta caattccgga cctttacggc gacggtgtag gtatggtata
     3361 tgatgaccct gtacagagtc tgcatataaa tcttccgccg ggagattatc agctgaacgg
     3421 tcagcaggct gtatggctta tgcgttacag acacggtaat gttaatccgt caaccggtgt
     3481 atttaaaggt tatgtaaacg gtgacagtga cagagttgaa atgcagcaga aattcctgaa
     3541 agctgttgta gaccaaaagg ttaatgcatc tttgattttg aaaattcctt caatattcaa
     3601 ggatataagc tctgaaatta agacaaactt tacagtaagt gaagttatta agtattcaaa
     3661 atatttggct gatttctcaa gcgtgaatat tcattcgtat tcattgcctg gtgaatatag
     3721 ttcagattca gcgaacggcg acgtttggat acctaatatg gacgaaatca gaacgatggt
     3781 acaagatgtg ttcggttatc ctgcagataa tataacaaca gacaatccta aaaatgctac
     3841 aagagagagc ggtagttctt catcaagcgg tatatcttca agctcgtcaa acacttcaaa
     3901 ttcatcaaat tcatcaaatt catcaaactc aaaaagcaga agttcaaatt catcatacag
     3961 ttcatcggga acttcgggtt catcaagctc aggtacctca aacagttcat caagctcatc
     4021 atc
//
LOCUS       DAWACS010000090         4020 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2436_length_4020_cov_1.974527, whole genome shotgun sequence.
ACCESSION   DAWACS010000090 DAWACS010000000
VERSION     DAWACS010000090.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2436_length_4020_cov_1.974527"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2792..2801
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3188..3197
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1133 a          859 c          615 g         1393 t
ORIGIN      
        1 cgttaccgtc gttgttgtcc cattaacgtc attcgttgcc tttgccagca tatcatcgcc
       61 gttgttatac tcaacagatg atggactttc tgtatatccc ttcacataat catatcttgt
      121 aggtcttata atattataag ttgtatagtc aacacaggta agactgttaa tgttatcatt
      181 gtctgttgca attgcataaa cactgtgcac acccgatgta acatcaggtg taaataccca
      241 tttaccgtcg tcacgttgtt tagcatcacc cgccttgatt tctccctcgt caatatatac
      301 ctcaacttta ctgacaacac catctgtgtc ataagcactt attacaatgt cgtcgcctga
      361 ctgctctact tttgatatat acggtgcata attatagaac tctgtcgttg acaactcttt
      421 atttacagct ttagaagcat atttctctgt caaatcagtc ataaacggtg ctataggtct
      481 gcctgcagca ttaaatgtat taatcacggc tggggtgtca gtttgtgctt caactaaatc
      541 ctttgcaata cccggagtat ctgaccatgc atacttaacc tgtggatttg ttatatctgt
      601 tacatctatt tctatagtat cgccgttaat tgtcggtgta acatctttgt atattccgtc
      661 atcatcttta agttcaaagc ccaacggcac acctccatca tcggttgaaa gagagccgta
      721 tgtattcttg aaatgaagaa taagtttatc gccactgcgt tccatataat caaaagatgg
      781 gctttcaaca tttgattgtg tattgttaat aaaatcttct atataagcaa ctgctctgtc
      841 agcaataggt cctttatcat ttgggtgaac attatttgta gttcctgtat cattgctcac
      901 aactgttttt acgttatcca ttctttgtga tacattccat tgtcccgctc ttactccggt
      961 gccaatgcgt attgtcgaat aaatttttgc aaaattcgca gtaggaagtt gaatgactac
     1021 aaatggtaag tcctcatcat taaatgtttt tctccagttg ttaataagcg aagtcaatgc
     1081 ttgctcatac actgtgccgc tttcaaaggt agtatttgcc tctccttgat accatacaac
     1141 agcagatgct ttcaaattct ttagcggcag aagtctttgt gtatatagtc cgccttttga
     1201 gcttgctccc gccatcattc tctttgcttg tgaatcccaa tttactgaat atgtcggaat
     1261 ccactgcatt attgacgacc cacccaaact tgaacttata agacctatag gaacatcaga
     1321 atcttttttt atcatcctct tacctatcaa gaatccaagt gcagaaaatt gctttgaatt
     1381 ttccatagta gcaaccttcc actctgaaat ctcgtcaaat gaattcatat atctcacgtc
     1441 ttcgtaagct tcacttaatt cttcgttcat caacgttgga aaagtctcaa ggcgattaaa
     1501 catatttgat tgtcccgtac agaatataac atcaccgaca gccacattat cgagagtaat
     1561 catgttatca ccgcttgata ctgtcatagt tgctgatttt acagcttcca tagcaggaag
     1621 ggttatctcc cataaaccat gttctattgt tgtttgctca tcagccccat taaaatttac
     1681 tgaaaccgta ttccccgatt ttcctgtacc tgtaatagta ataggttctt ttctctggag
     1741 taccatgttt gaggagtaca ccgcattcaa agttaactcc ggttcaagag taggcgttgg
     1801 ggttggactt gccgttgggg ttggcgttgg tgtcacatcc ggattgattg ttgtggttgg
     1861 cgttggtgtt gtatccggct ctgtttcgcc acctccatca ctgtctatat tcacatcaaa
     1921 tgagtcaata gaccactgta ttttcttagt gacttgagtt ttatcctcac ttaaatctcc
     1981 acagcctata tataaatata cgaatccatc ctttgatgcc gtaattccgt taggcaggga
     2041 atacttcata tttgatttgt ttgttgacca gttttttata ttactttcat ctgtatgctt
     2101 ttcaagttcc tcttttacca aatcctgcga gtgtgatgta agtgttattt cgctatcaga
     2161 aacaaaaaga caatattcca attccattgt tgcagtatca agatatgtaa aattaatatc
     2221 gacattaatc ttgtcgccgg tatttacttg tgccaactta aacattgtcg ctcttggtgt
     2281 attgtttgct tgtatataat aagtgacact ttctttactg cttacagtat tttttaatat
     2341 tttactatta cttttgtctg aacttggcag tgtatacgct gatactccgt ccgcttccgc
     2401 ttctactgta gttgtagtag ccgcaaatgc cgtcaaaccg atagatgcac atatcatcgt
     2461 cacagctaac atcaatgaaa taattttttt catttttttc ccctctttcc gtttttatat
     2521 acttttactc ataataacta tatcttgtta ttaatcttac cacgggaatt tttttattaa
     2581 aatgcggtaa actttaagct gtgcggttaa atttattaaa accaaccatg tttttcggta
     2641 caaaagtcat ataaatcgta aaatatgctg ttaaaagctt gacttgccat aacattatcc
     2701 ataaccctat tgacgcaaaa aaacagtgcc attagcgttg attttcaaac caacctaacg
     2761 acactgtttt attataaagt ataaataatt tnnnnnnnnn naatcgctat atcattccaa
     2821 aatgtttgag tgcgtattct atgccattat cgagcaaatc ttttgtcaca aaagatgccg
     2881 actttttaag ttcttcactg ccattaccca taacgatact gttaggcact gccgtaagca
     2941 taggcaaatc attcatactg tcgcctattg catacgcatg atcaatcgga atattatggt
     3001 attcaagaac tttttttatt cctgtaccct ttgaaaatcc ttttaccgtc atctcacaaa
     3061 agccgtcacc acgcacaata aaatcaaaat ctttttcgat ttctctttta aatcgttcta
     3121 tattgctttt ttcatcatac caagccaaga atttgtcaaa agcaaaacca tcgtcgctga
     3181 catcaggnnn nnnnnnntac cttgcatttc aaaacgtctt ttcagtttta caaaaccgtc
     3241 taaatttcta ctgcgtttat cacaataaaa tgacttagaa tgttcataca ttggtgtcat
     3301 attacattca aacacaagtt tagcaacatt tttgcaaagc tccgatgaca atgtatgctg
     3361 atatatcact ttgccatcac attctatata cattccgcaa ccacatatat atccgtcaaa
     3421 gcctatatct cttattctct gttcaacgtt cataacggtt ctgcccgtat caacatacat
     3481 caaatgtccg tttttctgtg ccttatgtat tgcatttacc gcactgtcgg gtattatata
     3541 tgcctcgtca tcagatataa gtgtaccgtc caaatcaaaa aatactatca taaaaatcac
     3601 tccaataagt atattatata tggtgcaaaa agatttttca agtcggacat atttaattta
     3661 tatcgcaagc attgccgcac ctataattcc cgcatcattg aaaagctgag ccgctacaat
     3721 ctttgtcttt gtcatatgtc tattaaaacc tgtattatac acatattctc gtatcggctc
     3781 aatgagatag tcgccctctt tacttatacc tccgccgata gcaattattt cgggctgaaa
     3841 aatattttct atactgacaa ttccgtctgc caaatatctc taaacacaca agtatgtacg
     3901 gaggaaaaat aaaccttggt tatcttacat atacaaaaat tcgtttctgg ggagaacaaa
     3961 cacttgcaac gcctaacgaa agatataatg gagattatct agctcaagtt cttacacctt
//
LOCUS       DAWACS010000091         4002 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2451_length_4002_cov_2.361794, whole genome shotgun sequence.
ACCESSION   DAWACS010000091 DAWACS010000000
VERSION     DAWACS010000091.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2451_length_4002_cov_2.361794"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1070 a          985 c          643 g         1304 t
ORIGIN      
        1 ttacgaaaag tattataatc aatgtggttt taaatctccc gaagaatttt acagtgaatt
       61 taaacagtat gcgaatatga ccgtagagga atactttaac ctcgtttggg caaaacaata
      121 ggctgtcggc aaaaaaacga cagcctattg ttttatttat tttcaatttt atacaacatc
      181 gttgccgctt cggcacgttt catattatta ttcggaagta tcgtgttatc ctcataaccg
      241 ctcacaatac ctgtcgaata caacttgctc acgccttctt ccgcccactg cggtatatcg
      301 tttttatccg caaaaggaag attaggcaaa tcatcattat ccggaagtat tcttcccaaa
      361 atcgtcattg cctctgcgcg cgttatattg tcatacggtg caaatgtgct tgttccgtcg
      421 tcattgacgc ggcctaaaat tataccctct ttatacacag cttttacagc cggaactgcc
      481 cactgtggta ttatatcatt gtccgtaaaa tcaagcttta catcatcata ttttgaaaaa
      541 tcaaattttt tgtaatttgc tatcatcgac gcaaattctg ctcttgtcat gttgtcaggc
      601 ttaaaaatca tttttccgtc ttcttcaaaa ccactgataa tccctgttgc cgccatatcg
      661 gcaattatgt cctttgccca gtggttttct gtatcgtaaa acggattagg tgtactcgga
      721 taatccgaaa tatgcaccgg tatttctttc gttaaatccc ctgccgtcac ctttatttta
      781 ccgtctttgt tgaccgtatc ggcaagtgtg aaaatgccgt cctctgttat cgtacctata
      841 tcaccctcaa cttcccattt atacaatctg tttgttgaaa gcaattcagt tccgtttttg
      901 tatgacgccg ccgaaagatt aagctgtaat tcttcgtttg cctctgtata tatttcgctg
      961 atttctttcc agtctgcttg attatatacc ttgatttctt caggtgtttc ataaacgccg
     1021 tatgtcacgt ctgccacgtc accgttatcg ccgtacataa caactttaac atcgcctgta
     1081 ccgttaagtg atactgttcc gttttcgtct accgtgctgt ccgtatcggt ttccgcacga
     1141 tattttatat tatacggctc ttccatttta taatttgagg tatcatatac gctttctata
     1201 ttaatattcg cggtcattcc cgaaagatag ttattattag ttccgcctgt aatattgatt
     1261 atccacggaa tattcgttct ctcgcgattg tcctttaaga aaatataatt tgcaacactt
     1321 ctcaaataac cgtccgacgg tgaatttaca accattgtat tgtcttttcc cggaaaccac
     1381 gcagaaatgg ttgtcgaacc gccgccgtca agatttaacg cgtcaacaca cccaagctca
     1441 accatacgtt tcgcaagcgt tttaagttga gcgccatagc tgtaacccga ctgtctgccg
     1501 tcaagagtat agaaaataat attgccgtct gcttttattc cgacagccgt tcttggtgcc
     1561 gcaccggctt caaaatcgga atttgccact ccgtttgtta caagtctgcc gccgacactg
     1621 cttaccgcgt tttcagccgt tttccaagta ccgtcatctc cgaccgcttg atttgcgacc
     1681 gtcaacgtct gacctgcgtg aagtctgctc aaaaagtcat aatattcact tacaccgctt
     1741 gtatccataa gcaaaacaac cttaccctca ggaatttcta tctcgccgtt atatatgaac
     1801 acatcatcaa ccgtaagcga cattgtttcg tccacgtgca gtctgccctc gttaggtgtg
     1861 caaataacaa aaatacattc cgattgagtt tttgtcgttt ctccgaattt gtccgtcaaa
     1921 agataaacag gatcaaatcc cgcctgacac cacttattta tatacataac gtcaatcgat
     1981 ttttcgccgt ccgtcactgt tgtctgaatg tcaagccact ttataaaacc cgttccgtca
     2041 cttctgaaac cgaccgcgtc ctgtccgccg tattcctttg atataatttc gccgtcggct
     2101 attgtataac ccatcggaat acctgtcttg aatgaaaaat aatcggcatt aattccggca
     2161 agcggtctta aaccgttctc ctgcatatat tgctctgcct gtttaatatt tctcgttccc
     2221 catatgctcg caccgttcac aacaatcggt acggcgtcct cattaggtgt atattcaaca
     2281 taatattcat tttgcttacc gacgctgtcc gaattgaact gtacattatg aaagtatgta
     2341 aatgctccca tatacgtact ccaaccgccg ttttgctcac ccaaaacatc tgcacttgca
     2401 gttattcccg taagcaacaa aagcgtcatt gcaaaaaata aaattttctt cctcacaaaa
     2461 cattcctcca tataaaaaac aatacatttt atagtatatc acaaaatcgg atttatgtaa
     2521 aacttaaata atggaatata tgcaattaaa tattatataa ttaaggctat cgacatcaaa
     2581 aaataaagta gacaaaacca tatcggtttt gtctacaatc caaattttgc ttaattctta
     2641 ttctatacct acaacttcat atcctgcgtc aacaacggct tttttaagca catcgtccga
     2701 cacgtctttt gcaagtgtta caacagcctg accgccgtta tcaagtgaaa cttccgcact
     2761 tactccgtct attgcgttta gcacctctcc aactctgcct gtacagtgtg aacacatcat
     2821 tccgttaatt ttaatcgtct ttgtcatagt tttattctcc ttttcatttt ttacagtttt
     2881 aatattattg cgttttggtt tgaaaaatct caatctcaac gcatttgaca caacgaatac
     2941 cgaactgaaa ctcatagcaa gtgcggcaat cataggattt aatttcaatc ctgctatacc
     3001 gtacaaaaca cctgccgcaa tcggtattcc gagtgcatta tagaaaaatg cccagaacag
     3061 attttctttt atatttttaa ttaccgagtg gcttagctct attgatgtaa caacgtcctg
     3121 caaatcgctt ttcatcaata caatatcagc tgattcaata gctatatccg ttcccgcacc
     3181 gatagcaatg ccaacgtctg cacgagtcaa agcaggtgcg tcattaattc cgtcaccgac
     3241 cattgcaacc ttgtgaccgc tctgctgaag ttttcttact tcttcttcct tgtctgacgg
     3301 caacacctcc gcaacggcac tgcttaacgg caacttgttt ttaattgcat ttgccgtaac
     3361 ggcattgtca cccgtaagca ttattacgtc aagtcccata ttacgcatat cttctatcgc
     3421 ttgcctgctt gtttccttta tggtatcggc aactgcgatt attccgacaa atttattatc
     3481 caccgcaaaa tacaatgatg tttttccgtt cttcgcgaat atttcatctt ccgatacttc
     3541 aatattgttt tcacgcatca tcttatagtt tccggctaaa actttctttc cgtcaacatt
     3601 gccttttaca ccttgtccga caatctgttc aaaatctgca acatcactaa attccaagcc
     3661 gtcggctttt tcaacaatag cctttgcaag agggtgttcc gacaaatatt ctattgacgc
     3721 tgcaatctgt aaaagttcct tatcggaaat tccgacaggt gctatatccg ttacagaagg
     3781 cttaccctct gttatcgtgc ctgttttatc aagtacgacc gtatcaacct gatgtgctgt
     3841 ttcaagactt tcggctgatt ttgtaagtat tccgtactgc gcgcccttgc ctgtaccgac
     3901 cattattgca gtcggtgttg ccaatcccaa cgcacacggg caagaaatta caagtaccga
     3961 tattcccatt gaaagtgcaa aactaacgcc tttacccaaa ag
//
LOCUS       DAWACS010000092         4001 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2454_length_4001_cov_2.610492, whole genome shotgun sequence.
ACCESSION   DAWACS010000092 DAWACS010000000
VERSION     DAWACS010000092.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2454_length_4001_cov_2.610492"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1066 a         1003 c          656 g         1276 t
ORIGIN      
        1 tgcaagtgcc tgaattaatg cagtaccatt gccactgaat gcatttacaa tgctgttcct
       61 gacattatca aacgcattat gcagtcgttc tattgccggc tgattatttt gaattgcatt
      121 ctttacagcg ttaaatactg tacttgcggt attataaatt gtctgaattg cagaaaataa
      181 tccgtttcct attgcaacag taacataaga aatagcggga gttaatgcac cgagtccgtc
      241 agacatagac tgaagaacgc ctttaaactg cgatgaaccg agtgcatcaa atatcgcaat
      301 tttcacaccc tcgaactgcg actttaacag tgtgagttta ccgcttaccg tgtcattcat
      361 agtgtcagcc atttttaaag ccgcaccttc cgaattattt atagcgtcag acaatgagta
      421 aaaatcatca ggacttgcat tcacgattga cagaaatccc gacagtgcat tttttccggc
      481 aatcattgtt gcatactgac ctttttgagc atcggtaagt gttgagaagc gtgtctgaag
      541 ttcgggaata agcacagata aaggtttcat actgccgtcc gtatttactg cagaaatccc
      601 caaagccgac atagccgcat ctacttcttt ggtaggtttc gccaaccttg tcataacacc
      661 tctcagagaa gtacctgcgt ccgaaccttt aactccggca tttgccatca gaccgagtgc
      721 cgtggtggta tctttcattg aatatcccat tgcacccgca gttgccgcaa catttttaaa
      781 ggactcgccg agcaaagata catttgtatt tgactttgac gatgcaaccg ccaaaacatc
      841 agcgaactca ccgctgtcac ttgctttcag tccgaaagcg gtcaaagcat cggttacaat
      901 atcagataca ccggctaatt cctcaccgct cgcagcggca aggttcatta ttcccgcaat
      961 accgtcaatc atctgcgatg aattccagcc agccataccc atatatgaca tagcctctga
     1021 cgcttctatt gcagaaaatt tagttttagc acccatttcc tttgctttct cggaaagtgc
     1081 agtcagttct gcacctgttg ctcccgagat tgcggatacg tttgccattc cctgttcaaa
     1141 ctctctgccg gtattcacta catctttgcc gaagtctatc gcttgacgta caccgacaaa
     1201 cgctcctacg gctactgccg ccttagatgc caacgaggtt ataaccccgc ctactccgga
     1261 agactgattt ccgaagtttt tcattcccga tgttgtaccg tttaaactgt ttctaaggtt
     1321 attgctcgca ttgacagcgg acttcatatt gacgaaaaaa ttaccgttat taaggcttaa
     1381 agttgcacct atattacgag ccaaatttta cgtcacccac tccccaataa cgccttatac
     1441 ttctccgtct cctcttccac agcaagttcc atactcgcct ttaaaaatat tttttcagac
     1501 agtgataacc ttgcaagcct gtcccaatca aatccctttt gaagataaaa atgaataagc
     1561 tgcaggtcac cgtccgtctg tattagtttt ttacggcttc cacaccgccc atatatccag
     1621 caagcttcat acactcaatc gcaatctgcg gtatttctcc cggtgcaaat ataatttcaa
     1681 caatatccat aggcactgca cagccgaatg cgtcctgtac ttccttcgac ttaatgtcag
     1741 gttctttaat acattcatag cacatatatt tatcaccctc gcctgcctcc atatcatttg
     1801 cgtcacggca aagtgctccg tccggttctt caatcgttat aaccgaatca atggacttaa
     1861 tatataaatc cattgtcttt ttaattttct ttgacgcaat catctgctcc ttacgtcgta
     1921 aaagttctgc taatgttaat tttgtagcct tattcatttt agtgtccctt ccttttttct
     1981 taataaaaaa ccagctgtat agccgtcatt ttttagtgtc cccatcatta aaaatacaat
     2041 ctttttatac acacgggtca gcgattgtat caagatactt aaatccgaca aatccgcccg
     2101 aaaattcatc ttcggtaatt tttccgttct caaatgcctg aagtgtaagt tcatcaagcc
     2161 agcatgacat aagctgtact ctttctgtac cgccgttatc ggggtcctcc aattttgata
     2221 taagcgacaa tcgctcgtca atacccgctg aaagtttctc cgcatacgtt ttgcctcttg
     2281 aataaatctt ttttattttc gctgtccaag taccggacac acccatcagt ttactgtcgt
     2341 cccacatttg ccccgaaaag tttatcgttt cacggtttgt tttgattttt gcttcaaatg
     2401 aagttatttc ataacaaata ctgttgttcc accaaaaata gccgtgcgta ccgctgatta
     2461 cttttccgac agcgggaagc tttctttctc ctgccattgt ttactgcctc ccttactcca
     2521 tatttataga aaatttcaaa tcctctattg catcacagaa tgttatatcc gccgttacaa
     2581 aaacataact ccccgtcttt gccttacgaa tctgttcatc actgtactcg gatatatcat
     2641 atttttgtgc aagccagtca cgctgtgcat taacatctat atctgctgta ttttccgcat
     2701 cgccgtacag tacgccttct cttacaagtc cgtcaaaata ctgattaata gcagctacaa
     2761 acataacctt attgtcatag ctgttattaa ttccgatata gttattctca aatgcagaac
     2821 gaatatcgtc acgcattaag tccataccct caataatttt gattttcttc atatcttcgg
     2881 tcttgtcacc gcttaagatg tgtagcgaat ttacacctcg tgcgactttt actttttcgc
     2941 cgtcattgat aagtataaac ttaccttcgt ctatatcttc attgggagta aggctctccg
     3001 ttatagagtc aatttccgaa agaacttgat atgtcgcact ctctgtcatt gacagtccgg
     3061 caagcaagcc tgcaattctt gcacaatatt catatgccga atatgtcttt gctcctactt
     3121 tgataccgct cgaggaaaag ttaacaatgc cctcattatt tgccgcagaa caaggcaaaa
     3181 ccgctttgaa tgtctttttt gccgctctct gtgctataat ccaattctgc agttcttccg
     3241 tcaaatcttt atgcggttcc aatcccggaa acgtcagcca gttccacgac ttatttctaa
     3301 ggcgtaccaa ggcggcgtta tatgactttt tgaagtcctc gcctgtttcc gctctttcca
     3361 caagcactcg tttcggtttg ccgagaaata ttttattcaa gtagtcaaga tttgtcgttg
     3421 tccagtctga tttcacaata tcggcttcat agttatatgt ataacttaaa ttttcatctc
     3481 cgactttggt tgaatcttca agaataaccg ctacaattcc gttctgactt cgtgttaccg
     3541 cagtctgtgc cttcgtctta aattcaatta aaatttcagg taatcccatt tttatccccc
     3601 cgtatcacaa gttcgctcat ttcatcatac ttttctgttt tatccacagc ctgaatgaaa
     3661 tttatatcaa aatacacata cattacaccc ttttcaattt caaaattcat ttcatgaata
     3721 gtcagatgtc tgtccataat atcgaaagtc gggtacaaaa aaagctcttt aatcctgctg
     3781 taagcaccta tacagtcctc cacagtttca agagccgata tatattttaa ttctaccgaa
     3841 acagtaagtt cctcaagtgc accgccgcag cactgaggct gtacatctga aggatacgca
     3901 gaaacaaaca ctgccggttt taaaaatccc tcatctactt ctgatgcaac tacgttaaat
     3961 ccggcatccg caaggatttc cgcagttcgt gtctgtatat c
//
LOCUS       DAWACS010000093         4001 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2453_length_4001_cov_2.906994, whole genome shotgun sequence.
ACCESSION   DAWACS010000093 DAWACS010000000
VERSION     DAWACS010000093.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2453_length_4001_cov_2.906994"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2553..2562
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1302 a          650 c          960 g         1079 t
ORIGIN      
        1 tcatcttgag ccttgggaag agggcaagca atatgacaga ctcggtatgg tggatgagta
       61 tacaaatata atgggtataa acgtaccgtc acttacgata cctgtaaaac ttggcagaaa
      121 ccttgcggta attgtcgaag ttgcgtcaat gaataacaga cagaaacgta tgggatataa
      181 tgcggctgtt gagcttaaca acagacttat gagccagatg gaaagtcagc ttggtaattt
      241 gtaaagagcc tgtcgacaaa tgttgacagc tccaaaccat tgtaattttt agataaggaa
      301 gatataacgt gaagatatta ttggacacac atacacatac attggcaagt acacacgcat
      361 attcaacagt tcttgaaatg gcgaaatatg cgtcggaggc aggaatggag gctattgcga
      421 taacagacca cgcgcctgct attcccgacg gtgcacaccc ttggcatttt cagaatttga
      481 aggccattcc gcgtgaaata tacggtgtaa agatattata cggtgcggaa gtgaatatac
      541 ttgacttgga gggtaatata gaccttgccg atgaggtact tgaaaaactt gacgttgtaa
      601 atgcaagtat acacgcacct tgctacaagg acggtgacgc gacagaccat acgagtgcgt
      661 atttggcgat agtcaacaac cctcttgtag atgttatatg tcacagcggt tcaccggcgt
      721 ttgtgtatga ttatgaaaaa atcatagacc ttgcaaagaa aaatcataag ctaatagaaa
      781 taaacaatca ttcgtttgac gtcagaaaag caagtattcc gaattgcaga aaaattgcgg
      841 aaatatgcaa agaaaaagaa gtcggaatag ttgtaagttc tgacgcacac attacgtttg
      901 atttgggtaa ttacaataat gcgcttggta tgctcagtga aatttcattc cccgaaaaat
      961 tggttataaa cagagattta aaatcatttg aagcatatat gaaagaaaag ggaaaagaac
     1021 tgtttaagtg gcatatgtaa ttacggaaag gcggaatata tgttagacag agaaagactt
     1081 tcggctatta ctgacaatgt tgtttacgat gaacctatga gtaagcatac aacattcaga
     1141 ataggcggca cggcagacgc gtttgtatcg cctgaaaacg cacttgaaat cgaaaaaatt
     1201 attcattttt gcaaagatac cgatacaccg tatatgctta tgggcaacgg ctcaaatatg
     1261 cttgtcggtg acggcggtat aagaggcgtt gtcatacaca tcggaaacgg tatgtcaaag
     1321 tgcagaattg agggtgaaga agtatatgcc gacgcaggaa tactgatgtc aacgctttca
     1381 aagaaaatcc ttgaggctaa tcttaccggt tttgaatttg ccggcggaat accgggtaca
     1441 ctcggcggcg gtatatatat gaatgcagga gcgtacggcg gtgagcttaa ggatataatt
     1501 gaaaacgtaa catttatatg tcctgacgga cttataaaaa ccgaaacggc agataagctt
     1561 gatttcggtt atcgtcacag tatgtttgaa aacggcggtt atactatttt atcttgtaaa
     1621 ttaaaactta aaaaaggcaa ttatgacgaa attaaggcat tgatggctga ttacaataaa
     1681 agacgaagtg ataaacagcc tttgtcacag ccgtcagcgg gaagtacatt taaacgtccg
     1741 gagggctatt ttgcaggtaa gctgatacag gacgcgggac ttatgggata ttccgtaggc
     1801 ggtgcaatgg tcagcgacaa gcacgcaggc tttgttgtga acaaaggcgg tgcaacggct
     1861 aaagatgttt tggatttaat taagtatgta caagataccg tggaagaaaa attcggagtg
     1921 aaattagagc ctgaagtaag gcttataggg gaacagtaag gagaagaaat cggatgcaat
     1981 atacaatagt aacaggaatg tcgggcagcg gaaagactaa agtaataaga tatttggagg
     2041 atatgggata tttctgtata gataatatgc ctcctatgct tataccgaag ttttcggaaa
     2101 tgctgacttc ggtaaacggt aaatttaata atgttgcgtt cgttgtcgat acccgtgtcg
     2161 gcgatatgat aaatgaactt ttggcacaga ttgatgattt aaagcaaaag ggatacgatt
     2221 gcaaactttt gtacatcaat gcggatgatg aaacacttgt aaagagatat aaagaaacac
     2281 gacgtatgca ccctattgaa aatacaaacg ggcttttggc atcaataaga caggaaagaa
     2341 aaatgctttc cgctctttat gaggcttcgg actatgttat agatacgtca catcttacgg
     2401 cacagggact tttgcaggaa ttaaaagaaa tttatgcgtc aggcgataca aagcaaggtc
     2461 ttgaaattaa tgtaatggca ttcgatttta aatatggtat gccgcttgac gccgaccttg
     2521 tgtttgacgt aagatgtttt ccgaatccct tcnnnnnnnn nngttctata ttgatgaatt
     2581 aaagcataaa acaggtaatg ataaagaagt tcaggactat gttatgagct ttccgaaatc
     2641 ggttactttt atggaaaagc tcgaagattt tatggaattt atgatacctc tttacattga
     2701 ggagggaaaa atttctctta caatcgcaat cggttgtaca ggcggtaaac acagaagtgt
     2761 tactatgaca aataaacttg cggattattt aaaatcatgt gactatacgg ttaatgtatc
     2821 gtatcgtgat atgggcaggg aatagtttaa aaggcgaata tttcgccgac gttagctttg
     2881 tgaaaggagt taatggcaga tgatttcaga tacaaaggtt gtggcgatag gcggaggaac
     2941 aggtctttcg actatgctca agggtctaaa gctacatact cataatataa cagcggttgt
     3001 aacggttgcc gatgacggcg gttcatcggg aatactcaga agtgacctcg gtatgcttgc
     3061 acccggtgac gtgagaaact gcgtaagtgc gttggcagag gttgatcccg taatgggcga
     3121 acttttgaat ttcagattta aagacggaag tttaaaaggt cacagtctcg gcaatctttt
     3181 tttggcggca cttaatgaaa cctcgtcaag ctttgatgag gcggtaaaaa aattcagcca
     3241 tcttgcaggt gtggtaggaa cggtgtatcc cgttacgaat gagtctgtaa cgctaagcgc
     3301 attgcttgag gacggcagac tgattgaggg tgagtcgcat atcggcgtac accatgacgg
     3361 ccctaaaaac ggtatagtca aattaatggt taatcccgaa aaaccaaagg ctgttgacgg
     3421 agttataaag gctcttgaaa aagccgatat aattgtttta ggcccgggaa gtctttacac
     3481 gagtattata cctaatttgc ttgtggacgg tgttgttgac gcaatcaaaa agagtaatgc
     3541 cgtaaaaatt tatgtgtgca acataatgag ccaggcaggc gaaacggaaa attatacggt
     3601 gtccgatcac cttgcggcga ttgaacgtca ttcgtatgag ggtattgcgg atatagttat
     3661 tgcgaataac gcaataattc ccgaaagtct tagaattaag tacgcgtccg aacatgcgcg
     3721 tgaagttatg attgactcaa taaacgttct caaaagagcg aaacttgtgc aaggtaatct
     3781 tttgcttgta cgaaacgata aaatccgtca caatttcagc cgtttggcaa gaactattat
     3841 gagattgggc agtgacgtaa attattttga aaataaataa gataaagata tgagaggatt
     3901 attttaatgt ctttttcatc acagataaaa gaaaagctgt gtaagagtga atacggttgt
     3961 atgaactgtg ctgcatatga attgtctgga gcattgatgt t
//
LOCUS       DAWACS010000094         3987 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2465_length_3987_cov_2.418108, whole genome shotgun sequence.
ACCESSION   DAWACS010000094 DAWACS010000000
VERSION     DAWACS010000094.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2465_length_3987_cov_2.418108"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2937..2946
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1199 a          671 c          936 g         1171 t
ORIGIN      
        1 tgtaaaatac attaataact atgtaatttg ggagagggtt taattggata tacatgaggc
       61 attgcaaacc gccggacagt atatttatat aggttttcgt gcattgcttg aggggctgca
      121 aaagctgttc ggaatgttgg cagattggtt tcaaagtctg ccatggccga atgcgataaa
      181 gcttttcagc aatgatacgg ctaataaata tttgtttttt ggtattgcaa tatacttgct
      241 tttgatgaat atatgggcat tcgcactgtt cggtgcggat aaatcaaatg caaagagaaa
      301 acagagaaga ataaaagaaa gtaaattgat gaaagtatgc tttttcggcg gtgcgacagg
      361 cggtattatc ggtatgaatg tgttccgtca caagacgctg aaaaagaaat tttcggtcgg
      421 tataccgatt ttgttcgtat tacagcttat tttaaacagt ttcatactcg gatttttggg
      481 attttggacg tttttctaaa aggactggaa ttttcaaaga aagtgtgata taataaaata
      541 agaatttgtc ggaaaggaag atacggcagt ggaattgaaa gatattttat cgacagacga
      601 taaattgaga attatacagg aacttgtacc gggtaaacaa attacccttg cacacgttat
      661 tgcaagtcct gaccctattt tatatacgaa gcttggactt aatcccgaaa ttgacttcgg
      721 aagaagtgca atcggaatac ttacaatgag tcctgcggaa acggctatta tagcggcaga
      781 cttggctaca aaaacatcaa atgttgattt aagctttgtt gcccgtgtca gcggtacgct
      841 tagaattacg ggtagggttg cggacgttga aacgtcactt cgtgcaatta tggactatgc
      901 cgaaagaact cttaaattta ctgtttgcga gataacaaaa acatgaagaa gataatactt
      961 atcggtagaa gtgagtgcgg taaaaccacg cttacacagg cacttaaagg cgaaaaaata
     1021 cattaccata agacgcagta tgtaaacaga tttgatgtta taatcgatac gcctggtgag
     1081 tacatacaaa ccaaaaactt gggaaatgcg attgcaatgt atacttttga agcggacgtt
     1141 gtgggacttt tgatgagttc aagagagccg tactcattgt atccgccatg cgtaacaccg
     1201 ctttgtaaca gacctgttat cggaatagtt acacagataa acagtgacgg tgctgatccc
     1261 gacagagcgg aaagctggtt aagacttgcc ggttgtgaaa cgattttcag agttgattca
     1321 aaaacgggcg agggcgtttg gaaaataatt gattatttgc gtgaggacgg cgatgtattg
     1381 ccgtgggacg aagaagaaaa aacaagcaga tagacgaaaa tgtctatctg ttttttcttg
     1441 cacaaaattt tgcaatttgc atatgatata gcataatatc gaatgggagg cagaggatat
     1501 gtgcggaata tgcggattta taaattttaa gacaaacctt gtcaagaacg aacgagaaaa
     1561 tcttgctgtt gctcaccgta tggcagaaaa actgcgtcac agaggtccgg actcttgggg
     1621 agaatgggtt ggcgaacacg cagtgtttgc tcatagccgt ttggcggtta tagacgttga
     1681 aaacggactt cagccgatga aacgtacagt cgagggacac gaatttgtga ttacatataa
     1741 cggtgaactt tataacacga acgacataag aaacgatttg aaatctcacg gatacgaatt
     1801 taccactgca tcggatacgg aggtgctatt gtatgcctac atacattacg gcgaaaaatg
     1861 tgccgaaatg ctgaacggca tatatgcgtt tgttatctgg gattctatgc gacaaagaat
     1921 ttttgcttgt cgtgacagat tcggcgtaaa accgtttttc tatacacaga aaaatgatgt
     1981 gacggtgttt gcgtccgaac tgaaatcact gtttgaatat ccggacgtat cggcagtgct
     2041 tgataaaacg ggactttgtg aactgtttgc gctaagtccc gcaagaacac agggtgtcgg
     2101 tgtgttcaaa gatgtaagag aacttcgacc ggcaagatat atgataatca accgtcacgg
     2161 aatgacaata aagaaatatt ggtcgcttgt gagcggtgag cataaagaca gttacgagga
     2221 aacgatagaa aaggtgcgtt cgcttgtgta tgattcggtg aaaagacagc ttatttcaga
     2281 tgtgcctatt gcaacatttc tgtcgggcgg acttgattcg agtattataa ccgcaatcgg
     2341 tgctatggaa atgaagaaaa aaggcgaaag actttcaacg tattcgtttg attacgagga
     2401 taataataag tatttcaaag cgtcgcattt tcagccggac agtgatacga agtgggtgct
     2461 gagaatggtg gaggcattta atacaaacca tacatatctt gtctgcccga ataacgtgct
     2521 tacggagctt ttggaggagg cacttttggc gaaagatttg ccgggtatgg cagacgtaga
     2581 cgcgtcactg ctgtatttct gtcgtgaggt gaagaaaaat catacagttg tgctttccgg
     2641 cgaatgttcg gacgaaatat tcggcggtta tccatggttt agggagaaga aagcgtttga
     2701 aacacacggt ttcccatggt gctatgattt gtcgataaga aataatattt tgcttgacag
     2761 tgttcgtaaa actttggata tagacaatta ttcgcgtatg cgatatgaag aaagtattgc
     2821 ggaagtgccg acttttgacg gagataatga tgaggaaaaa cgcagacgtg aaatttcgta
     2881 tctgaatata aattggttta tgacgaattt gcttgacaga aaggacagaa tgagtannnn
     2941 nnnnnntgaa gcggcaaagg gaatcttgcc tgaagatgta cgtctaagac gtaaaagtcc
     3001 gtatccgaaa acgcataatc cgcattatga ggaggcggtt aagacgttgc ttgacggtat
     3061 tatatccgat ccgaacgcac ctattttggc actttgcgat aaaactaaat taacgtcact
     3121 tttggacggc ggttcgtcag attacggtaa gccgtttttc ggtcagctta tggcaggacc
     3181 gcagtttata ggatatattg tgcagatgaa ttatttgctt agcgattata aagtgagaat
     3241 actgtaaaaa caaagagcag actttttttg tctgctcttt gtttttattc ctctttttct
     3301 tctttttcaa caagctccat acgattgata gatacctcgt atgcggtttt ggtgattgtt
     3361 tcttcttcgc tgatacgttt ttgatatgtt cgtgactgaa tacgtcccca tatttttacg
     3421 ttatcgccga cgtgaagatt ttttgcaaag cgagcattgc gaccccatgc aataatcggt
     3481 atgtagtcgg atttgttgta acttctgttt accgcaagca gaatatccgt aatttcacga
     3541 ccgaacggag ttgtgcggta aacgggttct ttacagataa atccgtttag gaaaagtgtg
     3601 tttggatttt tgctttcttc ttcgtcaaag tggcagatgt cttttgcaaa caccgtaaga
     3661 acaagacgat tgtcaccgtt ttcgtaactg ttataggaac ggaattgacc tgtcacctca
     3721 atcatatcgc cgatacagta ttcaccgtcc attataagtc tttcagatac cataactctt
     3781 acattatccg acgcaccgct taaacgaggt actttaacgg taaatgtata gaatttttcg
     3841 gcgtaaattt cgtggttgaa tacaaattcg tcctcaatag ttcctatgat tattgcctga
     3901 ttgtttgata agttgttatc catgtgtaga aaacctcctc tgctgtaatt aaatgctctt
     3961 tatcatcaag ttatatttat tcggata
//
LOCUS       DAWACS010000095         3961 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2484_length_3961_cov_1.896313, whole genome shotgun sequence.
ACCESSION   DAWACS010000095 DAWACS010000000
VERSION     DAWACS010000095.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2484_length_3961_cov_1.896313"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1445..1454
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1962..1971
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1308 a          588 c          930 g         1115 t
ORIGIN      
        1 atgcttgggt ttgacgataa gattgatgca gatgaaatga acggtatttt gcttaaatcg
       61 cttaatgacg taaatcaggg caaaagtccc gattttacag aactcgatat tgataaaaat
      121 gttgagtttt atattgtggg aattgctccg aatgtttcgc gtcttgcaca aaaatttata
      181 taccgtgata aattcggcaa catattttca catatagcaa gacatcaagc agatataatg
      241 attgttaatt caaaaggaaa tataccgatg tggcgaatat tccgagaatt aaaaccgcct
      301 caaagcacaa attatactac accgccgccg cttatggctt cggtgttcgg tgctataata
      361 aacggtacac gttatcccga gtccttgctt gaaaatgccg taagacgaat gaaaacggat
      421 aaatcggtaa attatgttcg tgcaggtatt atcaaggctt gtataaacag aaaagataga
      481 tattcaaaaa ataaggagga gataaaaatg gcacttgata ttgaaaataa taatcaagcg
      541 tatctgtgcg gcagactttt tgccgtactt gaaaaagttc aatcggacgc attgcctgga
      601 ttgatcagaa cgattaagga ttcatatttt gcgtcagctt gtgcaaatcc gtcaacggta
      661 tttccgaaac ttataaagct ttcgcaatac cacattgaaa aacttgaata taaagttaaa
      721 tataagatag ttacgggtga aattatggac aagctaaacg gcaaatttcc aaagacatta
      781 ccgcttagcg agcaaggtaa gtttattata ggctattatc agcaaacaca agatttttat
      841 aagaaaaaag aagataagga aatatgaaag gaagtagaaa attatggaac caattaaaaa
      901 cagatacgaa tttacagtta tttttgatgt agaaaacggc aaccctaacg gtgatcccga
      961 tgcaggcaat atgccgagaa ttgatcccga aacgtcggca ggacttgtta cagatgtgtg
     1021 cttaaagaga aaaatccgca actttgtgga aatttttaaa gaagatgagc cgtattataa
     1081 tattcttgta aagcctgata aggcacttaa cacaaaattt acggaggcat atgaggcaat
     1141 aggcgaaaag acaggtcaaa agggtaaaaa tcctgaggcg gtaagagcgg caagggatta
     1201 catatgtcaa aattattttg atgtaagaac attcggtgcg gttatgagta caggtaacga
     1261 cccatgcggt attgtcagag gtcctgttca gattagtttt gcaaagagtg ttgatccgat
     1321 atttacacag gatataacaa ttacaagaca ggcaagaaca acagaagata agcgtgatac
     1381 aggtgaaacg gaaatgggca gaaagagcat agtaccgtat ggcgtgtaca gggcggacgg
     1441 atatnnnnnn nnnncagaaa attactaagt tttcggaaga agatcttgaa cttttgtgga
     1501 aggccattat caatatgttt gaacacgaca gaagtgccgc aagaggtaaa atgtgcgtaa
     1561 gaaaacttta tgtattcaag catgattcct tactcggaaa tgctccggcg aatgtgttgt
     1621 ttgacaaaat tgttatcggc aaaaaagacg gagtggaagt tccgagaagt ttcaatgatt
     1681 atgaaatcac tgtcgataaa aatatgcctg aaggagtaga acttcaggag aaagcatgat
     1741 taatataagg acaatacagc attatatgta ttgcccgaga agattcggat tgcttgaaat
     1801 tgagaatgct tgggttgaaa atgtttcggt tgtgttggcg aatattatgc acgaaaacgt
     1861 acattcgggc aaacaccaat tcaaaagcaa taacaaggtt gttttaagct cggttacttt
     1921 gtataatgat gaacttgatt tgtatggcgt tgcggattgt annnnnnnnn nggattgtat
     1981 agagttttgc aaaaataaag acggcgtacc gatagacggc cttgacggaa agtacactgt
     2041 cagacttgtg gaatacaagc ctacacagcc taaagacggc tctataagag aaactgacgc
     2101 gatacaggtg tttgctcaaa aattgtgtgc ggactatatt tggaaatgca acagtgaggg
     2161 gtgcatatat tatgccgata caagaaaacg tgtgaaaatg ccgtttgatg aggagtacga
     2221 caggtataaa gcattgcttg atgatttggt cggtaaaatg caaaacgtaa tggaaagcgg
     2281 tgttattcct ccaaaaataa aaggtcaaaa gtgcagtggt tgctcgatta aagatttgtg
     2341 tatgcctaaa acgaaaaagt acagcattaa acagcttatt gaggaggatt gcgtatgaga
     2401 aaacttttga atactttgta tgtcacaaat gaaaatatat acatgactct cgacggcgaa
     2461 aatgttgtat gtaaggaaaa taatgaagtt aaattacgac ttccgttttc aaatgttgag
     2521 gcaatatttt gttccagcta tcttggctgt tcgcctgcac tcatggggaa gtgtgccgat
     2581 tacggcatac cgattaactt catatctccg tcaggcagat ttttggctcg cgtgcagggt
     2641 gcgtcacacg gaaatgtgtt gctgagaaaa cgtcaatacg aacttttttc aaaaccgcct
     2701 gcacttttaa gacaaaatac cgttgccgca aaattggcga atacaaaaag tgttgtgaaa
     2761 agaacgctta aagattatcc gcaaattgat gaggacggca agctgactca ttgtatagaa
     2821 cgcttggacg gttgtattaa aagcgtgtat atcacagatg attatgatgg tatactcggt
     2881 acggagggta acggtgcaaa ggcatatttt gatgtgtttg acagccttat tcttcatcaa
     2941 aaagacgatt tctgttttgc aatgagaagt aaaagacctc cgctcgatag ggtgaatgca
     3001 atgttgtcgt ttttatatac gatttttatc cgtgaatatg cggcggcact tgaaagtgtg
     3061 ggattggaca gttatatggg attttatcat tcgcttcgtc ctggacgtga atcgcttgcg
     3121 tgcgatttgg tcgaggaggg gagatgtatt gttgaaagat ttgtgttgac attaataaat
     3181 cttaaaatta ttaaacccga agattttgac gtacagttaa gcggtgcagt tttcttgaat
     3241 gatgacggca ggaaaaaggt gctctcaaaa tggcaggaga aaaaacgtac cgatatgata
     3301 catccgtatt tgaaggaaaa aattcctatg ggacttttgc cgtatgtgca aagtatgttg
     3361 cttgcaaaat ttgtgcgtgg tgaaattgac gagtatccgt gttatttggt gaagtaggtg
     3421 atattgatga tgactttggt aagttatgat gtgtcaactg tcgatgcggc aggcaggaaa
     3481 agacttcgca aggttgcaaa ggaatgtgtt aattacggac aaagagtgca aaattctgtg
     3541 tttgaagttg atgttgatta cggaacattc ttgaaattaa aagatagact tatgaaaatt
     3601 attgatgaag aacaagatag ccttcgattc tattatttgg gtaataattg gaagaggaga
     3661 gtggagcata taggtgcaaa ggaaacatat gaccctgagg gcagtttgat tttgtgagcg
     3721 aacgtgcagt gatattggtt ttatatggag gttcgctgaa aaaataatac atattatgta
     3781 aaaaatatcg ctttttaaag tgatttttag taaaatgtcc gctctgttgt ggtggtgtga
     3841 cttcacatat acaatatgta gtgtcacgct ccccacggag cgtgtgagtt gaaatgtaga
     3901 tgtattcatt tattatcgct tgttggatgt cacgctcccc acggagcgtg tgagttgaaa
     3961 t
//
LOCUS       DAWACS010000096         3943 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2502_length_3943_cov_2.616770, whole genome shotgun sequence.
ACCESSION   DAWACS010000096 DAWACS010000000
VERSION     DAWACS010000096.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2502_length_3943_cov_2.616770"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1298 a          649 c          925 g         1071 t
ORIGIN      
        1 tgcttgcggt tgcaaaggat atggtataca ggacaattct gtggagcttt gcgtacaagg
       61 attgggacat aaatatgcag cagggggcgg actatgcgtt taatcaagtt acgccatatc
      121 tgcatgacgg agctgtttta cttcttcacg cagtgtcctc cgacaatgca aatgctttgg
      181 aagatataat aaattacgca aaaaatgagg gatatacgtt taaaagcctt gatgaattgc
      241 aataattaag aaaaaaacat aaaaactctt aaaaaacact ttattaattc atcattttgg
      301 tgtataatta aattattata atgtatttta gagaggaaat tgataaaatg aaagaggcaa
      361 gttttgattt tggtaagaaa ccgcctgttg acggttacac aaaagtgact gaaaaatctg
      421 tttacacaaa ggaaaaaggt tttggacttt cggaagccgc agaggctgac gaaagaaaaa
      481 tcggtgaaaa ggaattaaac agagattttc tttttatggg cggaaagtca tttatcgttg
      541 atattgaaaa cggtgaatat attgtacgcg tttctacggg cgactatgtt gacgaaggcg
      601 atgttatgac attctacaac gtcaacggtg aaaaatacgg cgtatgggta agtgacggta
      661 ctgttgtgga aagagtgttc cctgttacag ttactgacgg taagattgaa tttgcatttg
      721 aaatgggtaa gcatacttgc cttaacagca ttgatattgc acaaaagcaa gacattgaag
      781 ttaagaatgt taaaagtgcc gttatcgcaa aacgtgatac agcttcggtt aaattgactt
      841 gggataaagc cgatggtgtt atcggttaca gagtgtcacg cagaaatcct aagaataatg
      901 aaattgataa ggttcaagac gttgttacag aggaatttgt tgacggtgac gttacaatat
      961 gcgataaatt tgaatacagc gtgtgtgcat tgtatgcaca taagttctgt tcggataagt
     1021 ctgttacgat tgatgttgaa gtcgttgacg gcaagagcgt tgccggtgag ataacagagc
     1081 ttgatgcaaa ggaaactcct aattccgtaa cacttgtatg gaacggattt aaggaggcgg
     1141 tttggtacaa catttaccaa aaagcacctt acggtattta caagtacata ggaaaaacag
     1201 aagaaacaca tttcattgat gataaagtaa taacaaacgt acctttcgta tatgcggttg
     1261 aggctgttac gacaagcggt atatcaaaac gcagtgaggt aacaatagat atggaggcta
     1321 agccgaagaa acgcaaaatg gaaacgttgg gcagaggtgc cgttgcaatg atgacagaaa
     1381 acggcgtatt ccttagctgg cgtcttaatg cgtatgaata cgaacaggac attaacttta
     1441 taattttaag aaacggtgaa aagattacag acgtaatcac cgattctaca aactatcttg
     1501 acaaagacgg taaaccggaa gacgtttaca caatcaaagc cgtaaagggt aataaggctg
     1561 agaaaaaggg tgcggaagtt aaagtcgtaa atgcacctta tatatcaatt cctcttgaca
     1621 agcctgaaaa ctttattgac cctgacggca attcatatcc ttataccgca aatgacgcgt
     1681 ctgttgcaga ccttgacggt gacggcgaat acgaaatcat tcttcgttgg gacgcaaacg
     1741 gtaaggataa ctcacacaag ggtataacgg gtgaatgttt gcttgacgca tacaagcttg
     1801 acggtacaaa gctatggaga attaatctcg gacgtaatat ccgttcgggt tcacactata
     1861 cacaatttat ggtgtatgac tttaataatg acggtaaggc agaacttgtt tgcaaaacag
     1921 cagacgcaac tgttgacggc aagggtaacg taatcggtga caaggacgcg gattacagaa
     1981 ataaggacgg tttcattctt gaaggccctg aatatttgac attgttcaac ggtgaaacag
     2041 gcgaaattat ggatactgtc gattatgatc cgccgagagg caacgttcgt gaatggggcg
     2101 actcttgggg taacagagtt gaccgtttcc ttgcctgcgt ggcatatctt gacggtgaaa
     2161 atccgagcgt tgtaatgtgc agaggttact atgaccacgg ttgtccgaca gtgcttgttg
     2221 catatgacgt aattgacaat aaacttgtta agcgttggaa gttccttgct aacaaggatc
     2281 agaatatcga atacacaaat caaggtaacc acaatcttgg tgtcggtgat attgacggcg
     2341 acggacttga cgaaatcgtt tacggcgcaa tggctgttga ccatgacggt aagggtattt
     2401 actcaacagg ccttgaacac ggcgactgta tgaaccttgg taacttcaca aagaaaacac
     2461 ctaaccttga cttcttccaa attcacgaac acgacagtgc cgaatacggt tttgaagtgc
     2521 gtgaccctgc aacaggcgag attaagtggg gtaagtttac aggccgtgac acaacaagag
     2581 gactttgcgc gaagattgac ccgagatatg aaggcaatca gtgttgggta atggacgacg
     2641 gtatctacac aatggagggc gaacttatca atgaaaaagg ccctgaatca atagattttg
     2701 ctatttggtg ggacggcgac cttatccgtg aacttcttga ccacgaattt gatgatgaaa
     2761 aggcagtcgg ttatccgaaa atttataagt gggattatga aaacaacaaa cttgtgacaa
     2821 ttcttgaccc taagggtact ctgtcaaaca actggaagaa gggtacacct tgtattcaag
     2881 ctgatatttt gggtgactgg agagaagaag ctgtttggag aaatgaggac gatacggaac
     2941 ttcgtattta cacaacaaca gatcttacag accacaagtt ctatacgttt atgcacgaca
     3001 gtgtttacag attaagcgtt gcattccaaa ataccgctta caatcagtgt acacaaacag
     3061 gtttctatat aggccctgaa atggataaac cgcctgtacc taacaacgaa tatgtgagag
     3121 gaattaacat tcctgacttt acggaagata ttgatgaaat ttagcattta atgtatacta
     3181 tgtatcaaat cctttacaaa acgattgata ttttatgtaa attaggaaaa aaaacaataa
     3241 cattgttgac cttttgcaaa aagtaggtta gaatatagaa gaataatatg gaggattgtg
     3301 catgaggata atatctggaa tcagacgcgg acataaactt tttgaatttg agggtgatga
     3361 tgttcgtcct acgactgaca gagttaagga gtctgtattt aatataattc agacgtttat
     3421 acccgatgcg gtgtgccttg atatgtttgc cggcagcggt gctttgagct ttgaggcgat
     3481 ttcacgcggt gcaaaaaaag ccgtttgcct tgataaagac aagcgttcga ttgatattat
     3541 caagaaaaat gcaaattctt tggatttttc ggattactgt gaaattatta atacgtcatg
     3601 ttttgattat atggagtggg caaaggaaaa attcgatgtg attttccttg accctccgta
     3661 taataaaaac tttatcgaac ctgtacttga gggtattgtt aaaaacaatc tgttaaacga
     3721 ggatggtata gtggttctgg aaagtgacgg aactgatttt catgatgatt ttgacggact
     3781 tgaaatgtac agacagagaa agtacggcag aacatatatc acggtatata agaaacataa
     3841 ttgagcttgt cgctaaaatg tcggcagcta cccaaattac atcatacgat gtaagcaatt
     3901 aaatcggctt ttgtgggata agcctaagag gaaagaagaa caa
//
LOCUS       DAWACS010000097         3942 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2503_length_3942_cov_2.824801, whole genome shotgun sequence.
ACCESSION   DAWACS010000097 DAWACS010000000
VERSION     DAWACS010000097.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2503_length_3942_cov_2.824801"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    667..676
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1269 a          636 c          991 g         1036 t
ORIGIN      
        1 gaaacccgag ctttacgtgc aggagcttgc acttttaaag gcgagtgcaa ccgcgaaaga
       61 ggttttacgc aataaaaatg cggttataat ttcagcagac acaatcgtaa cgcttgacgg
      121 acaaatactc ggtaagccga aagacgaaga tgacgcattt aatatgcttt caaaactttc
      181 gggacgtgaa catgaagtat atacggggta ttgcgttatg cgaataagcg acggaaaagc
      241 cgtttgcgga aaagttagga caaaagtaaa gtttaaagat ttgagcgatg acaaaattcg
      301 cggatatata aattcgggcg agcctatgga taaggcaggt gcatacggaa tacagggact
      361 cggttcactt ttgattgaaa agattgacgg cgattatttt aatgttgtcg gtctgccgat
      421 aagtgcactt gcggacacgc ttgaggacga aatggatata aagatattat aaaaggagaa
      481 caacgaaaat ggggatattt tcaaaagata taggaattga ccttggtaca gcaaacacac
      541 ttgtgtatgt aaagggcaag ggaatagtag taagagagcc gtctgttgtg gcgattgaca
      601 agtacgaggg taaggtgctt gcggtcggta atgcggcaaa tgagatgata ggcagaaagc
      661 ctgaaannnn nnnnnngcta tgataagagc gtttataaaa gaggcagacg tgagcaacgt
      721 atttaagccg agagttgttg tttgtatacc gtcgggcata acggaagttg aaagacgtgc
      781 agttgaagag gcggttattc aagcaggcgc aaaggcagtt gcacttatcg aagaacctat
      841 ggcggctgct atgggtgccg gacttcctgt gaatgatgca acaggtaata tggttgtcga
      901 catcggcggc ggtacaaccg aagttgcggt tatttcgctg ggcggtatcg tttcgtcaag
      961 atcaatcaga attgcgggaa atgctttgga cagtgctatt atcaattatt tgaagaaaga
     1021 aaatcaaatc aatataggtg acaaaatggc tgaagatata aaggtagcca ttgcgtctgc
     1081 atatgaagat gacgaagaag gcgtttacga agtaagaggt cgtgacgttg cgacaggctt
     1141 gccgaaaacg gcacaaatca aggaaagcga aatcagagcc gctataagcg agaatgtgga
     1201 cgaaatgatt gaagctataa agctgacatt ggaaaacacg cctccggaac ttgcggcaga
     1261 cgttatggaa cgcggaatag tgcttacagg cggcggtgcg ttgattaaag gtcttgacaa
     1321 acttatcacc gaaaccacaa aaattcctac acatgttgcc gaatatccgc ttgactgtgt
     1381 tgcaatagga acaggtaagg ctcttgataa tataaaagaa cttatcgaat catcaaaata
     1441 attttggatt gtataacgga aggttagaaa atatgtcatc attttttaaa agaaaaggcg
     1501 tgattgcggc ggcgattata accgccgtag tgcttgtatg tgccgctatt gctcatttcg
     1561 gcggtacaaa tcccgtatcc aaagtgctta gaactgtatt ttcgccgttt cagaacggtt
     1621 tttcatatat agtgaacaag gttacggata cgactgattt tattcgtgaa atgaacggct
     1681 acaaggaaga aaacgcaaga cttgtgggcg aaattaatga gctgaaaaaa caaaataaag
     1741 atgttgcaaa ttacagagaa gaaaacgaac gtttaaaaga aacgcttgaa cttcaaaaca
     1801 gtcttgaaaa ttattcaacc gttgccgcgt ctgttattgc gtattcgtca aataactggt
     1861 acgacacaat acagataagc aaaggctcac ttgctggtgt agctgtcgga aacgcggtta
     1921 taacacctga cggagtagta ggaaaagttg ttgaaacagg tcctacatgg tcgattgttt
     1981 cgacaatatt gaatcccgat aacgcaatgg gcgtaaaggt ttcaagaaca agcgatgttg
     2041 cggttgttga aggcgacagt gaattgtaca gccaaaacta ctgtaaaatg acgtttattg
     2101 acaaaggctc aaaccttata ataggcgata ttcttgaaac gtcaggttca ggcggaattt
     2161 atccggcagg actttctgtc ggcgtaattc gtgagataaa ttcggacgct atgggtaatt
     2221 tgaattatgc aacagtagag ccgcttgttg atttcaacaa gctttatgaa gtgcttgtta
     2281 taaacggaac atattgattg gagagaaaaa gtgagaaatt ttaagatttt tctgtggatt
     2341 tttgtgattt ttcttgttca gacggtagtg ctttcaccta tccatatatt cggtgcggta
     2401 ccgtctgcgg tattggcgtt tgtgatgtgt gtggcaattc ttgaaaatga atttcgcact
     2461 gctgttataa taagcggaat atgtgccgtt gttatgggcg caatcggagg aagaaatttt
     2521 acggagatta cgttgtttta tgcgtacagt tcgataatcg tattcgcggc aagaaaacgt
     2581 ccgagatatg tcggtaattt tccgaaaacg attgtatgga cctttattat gtcggcaata
     2641 cttgaaatat tgttgttcgt cataagagaa atgacgcttg atgtgtcggt aattttcagt
     2701 gatgcattgc cgacggcggt atttaatacg gttattgcag ttatattgta tccgatacta
     2761 aaaaagacat tgtataaaga agaaaagaag aaaaagcttt tgatagctta aggagaatgt
     2821 atataaatgc caaacagtaa agcgagattt attatattaa aaatagtcat agctgttatg
     2881 tttgcggcgg tgatatggaa gctttttgat ttacaggtat taaagggcga acagtattat
     2941 gaaatcgcta atgacagaat gactaccaat atagtcgaaa aagcaccgcg aggtgaaatt
     3001 cttgacagat acggaacaac tcttgtatcc aacaaagtcg gttattctgt tgttatgcag
     3061 aaaaccgatg ttaaagacga agaatttaac gatattataa aaaaacttgt ggatatattt
     3121 tattcgactc aatgtgaata ttatgacgat ttaccaattt cattcgcacc gtatcagttt
     3181 aagtttgagg acgaaaacga ggacggctcc gtggaggacg aacgtgaagc gtggtttaag
     3241 aataattcac atcttggcaa gggtatagaa gaaaatatgt ctgccgacca aataatgcag
     3301 gcatacaagg aaatatataa agtcagcgat gtttacagcg aggacgagca aagacgtatc
     3361 gtgggtatcc gatacgctgc ggaggcaagc ggtttgtcac agacaacatt gtttacagtt
     3421 gcggacgata tatccgtcga tgcggtaacg cagataaagg aacgtcagga cgaatttaaa
     3481 ggcatagccg ttataaacga ctatatccgt caatatgacg ctcccggact tgcaacgcat
     3541 attttaggtc gtacaggtaa aatcaatgcc gaagaatacg aggcaaacaa agatttgggt
     3601 tacggctata atgacattat cggtaaacag ggcattgaaa agtggggcga acagtattta
     3661 agaggtattg acggcactac aggtacaact aaggaagtaa acggtaaaga gattaccgtt
     3721 atgaacgatg cagagcctgt tccgggcgat tatatcgtgc ttacattgga ttcggatttg
     3781 cagaaagtgg cggaaaaatc gcttgcagat acaatacaaa atattcgtgc gtcaagcggt
     3841 gtgaaagcaa aggacggtgc agactgtgac gcaggttctg ttgctgtcat tgatgtaaag
     3901 acaggcgatt tgcttgcagg tgcgtcgtat ccgacatacg at
//
LOCUS       DAWACS010000098         3928 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2516_length_3928_cov_2.435838, whole genome shotgun sequence.
ACCESSION   DAWACS010000098 DAWACS010000000
VERSION     DAWACS010000098.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2516_length_3928_cov_2.435838"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1157..1166
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3291..3335
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1207 a          786 c          767 g         1113 t
ORIGIN      
        1 aggacgcact gaacacagct gagaaatttg tcggtacaaa aggattggct tttgaaagtc
       61 tttcggaaaa taccgcaata ccgtcataca catttgtgaa aaacaccgac tcaaatcaaa
      121 tttcaaaaag tataacaaaa gacggcggat atattctttc atatctgaac agcagaaata
      181 ttgacggcga acgtcttgac attaattccg caacagacaa agcggctgaa tttttgcaga
      241 aaaacggttt ttacagtatg cagagcagtt attatgaaaa atcaggcggt atcgcaacga
      301 taaactttgc atatgtacag gacaacgtaa cgtgttacag tgaccttgta aaagttcgtg
      361 ttgcactcga tacaggtgaa attgtaggtc ttgaaacaaa cggatatatt atgaatcacg
      421 cagagagaaa cttatcagcc cctgcacttt ccgttgatga cgcaaaatca aaaatatcca
      481 caggacttga cgtgaccgca acaaaacttg ctcttgttcc gaaggacagt atgagagaag
      541 tgctttgcta tgaatttaag ggtgctttta acggcaaaaa ctttttaatc tatgtcaatg
      601 ccgaaaacgg tcgtgaagaa gaaatattcc tgcttatcga atcggaagaa ggaattttga
      661 cagtataatt taatatattg actttttcgt gtatttacga tacaatatat aatagtatat
      721 tataggtaca ttgtaaatat aggaaaggaa ataaaaccaa tgagtaaaag attatttact
      781 tcggaatctg taacagaagg tcatcccgat aaaatctgtg accaaatttc tgacgcaatt
      841 cttgatgaaa tactaaaaga agatccgatg gcaagagtcg cttgcgaaac tacttgtaca
      901 acgggtataa tctcaattat gggtgaaatt acaacatctt gctatgttga tttccctaag
      961 gttgcaagac aggttgttct tgacatcggc tatgacagag ctaaatatgg ctttgacggt
     1021 acaacttgtg ccgttgttac ggctattgac gaacagtcgc ctgatattgc gcaaggcgtt
     1081 aatgacggtt atgaaaaccg tgaacaaggc ggtaatgaca acgaaaattc aacaggtgcc
     1141 ggcgaccagg gtatgannnn nnnnnnaact tacacaagta agaaaagacg gtatccttga
     1201 ttatcttcgc cctgacggca aaactcaagt tactgttgaa tatgatgacg ataagcctgt
     1261 aagagttgac gcagttgttg tatcaagcca gcattctcct gatgttgata ttcaaaaact
     1321 tcgtgaagat attaagagag aagttatttt aacaactgtt ccggctgaac ttatcgatga
     1381 aaacactaag ttttatatca atccgacagg cagatttgtt gtcggcggac ctaacggtga
     1441 cagcggttta acaggaagaa aacttatcgt tgacacatac ggcggttatg caagacacgg
     1501 cggcggtgct ttttcgggta aagaccctac aaaggttgac agaagtgccg catacgcatc
     1561 tcgttgggtt gcaaagaatg ttgttgcggc aggacttgca aagaagtgcg aagttcagct
     1621 tgcatatgca atcggcgttg cacaccctgt ttcaataatg gttgatactt tcggtactgc
     1681 aacagttgat gaagataaga ttgaacaggc aatcgaaaaa gtatttgatt taagaccgca
     1741 cgcaattatc aaacaacttg atttgagaaa gcctatctac aaaaaccttg ccgcttacgg
     1801 tcatatggga cgtgaagact taggcgtaag ttgggaaaaa cttgataaag ttgaagaatt
     1861 gaaagctgca attaaataac taataaaatc cgatggcatt aaccatcgga ttttttatgc
     1921 actcaaaaag cacatcttac gatgtgcttt ttgtaataca aataactttt tcattagaaa
     1981 tctacttctg taccgtagta tgtgcaagta aacaatttct ccatctgttc cggagtaatt
     2041 gaacgtgggt ttgaacctgt gcaagcatca cctactgcaa gttcggcgat tgatgacaat
     2101 ttttctttaa attcatcttc cttaacaccg aattcctgca atgtcttagg gatattcata
     2161 gctctgttaa attcttcaat cttagcgatt agagcattta cctgtgcctt ttcagatgtt
     2221 ccgccaagtc ctacacgtct tgcaatatct gcataacggt gcatagcctc tggctcgtgt
     2281 gcgttgtact taattacata cggcaaataa attgcatttg cacatccgtg aggaatatga
     2341 cctgttgaga atgccgcacc tgttttatgt gccattgagt gaactatacc aagcaaagca
     2401 tttgagaatg cctgacctgc aagacattgt gcatagtgca tttgttctct cgcgtccata
     2461 tcgcacttat acgactgcgg taagtaatca agcaccatct ctattgcctt aattgcaaga
     2521 gggtctgtaa acggtgaatt aagcgttgat acatatgcct cgattgcgtg agtaagtgcg
     2581 tccatacctg tgtatgcaac ttgctttatc ggcaatgatt caacaagctg tgggtctacg
     2641 attgcaacgt ccggtgtaat ctcaaagtct gcaagaggat acttgatacc ctttgcataa
     2701 tctgtgataa ccgaaaatgc agtaacttca gtagccgtac ccgatgttga cggaatagct
     2761 acaaatttag ccttttggcg aagtttcggg aatgagaaag gtgtacagag ttgctcaaaa
     2821 gtaacctccg gatattcata gaatgcccac atagccttag ccgcgtcaat cggtgaacct
     2881 ccgccgattg ctacgattac atcaggctga aaatctgtca tagcctttgc acccttcata
     2941 acagtttcaa ctgacgggtc aggctcaaca ccttcaaaca actgtacttc cattcctgct
     3001 tcttttaaat atgcttctac tttatcaaga aaaccgccgc gacgcattga actgccgccc
     3061 gatacgacga tagctttttt accgtcaata ttctttaggt tttcaagtga atcctttccg
     3121 taatacaaat ctcttggtaa tgtaaatctt cccataataa ataacctcct ctaataataa
     3181 gaaaaaagta gttgttaaat tattatcaat ctaacaacta cattatatca ctttatattc
     3241 agtttttcaa tgatttaaac ctattttaca aaaaatttac cttttgcata nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnctcgt tgcatcgaca atggtcgtta
     3361 taagcggtgc cgccataaca gcggggtcaa gcttacaacg ctttgcaagc ataggcagtg
     3421 aacagcctat aaactgcgaa attatgacta taaatataat cgacagactc accacagtcg
     3481 caatttgcaa atcacctgac attatccata ttcgcacacc gtttacgatt gccaatataa
     3541 cgcatattac aagcgaaatt ctgaattcgg taaatatgac ttttaagaag tctttcagtt
     3601 tgatttcgtc aaccgacatt ccgcgaatta tcattgttga gctttgcgaa ccacagttac
     3661 ctcccgtgct cataagcata ggaataaacg atacaagtgc aggtatcgcc gcacacgcgt
     3721 cctcatattt tgtcaaaata gttcccgtta tcgccgccga caacatcagt atcaacagcc
     3781 aaaaaattct atttttggcg tgtttaaaat cagacgtttt gaaatacgat tcctccgtcg
     3841 gattgatagc cgccattctt tcgatatcct ctgttgcctc gtcctgcata acgtcgattg
     3901 cgtcgtcaaa ggttacgata ccgacaag
//
LOCUS       DAWACS010000099         3897 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2543_length_3897_cov_2.246486, whole genome shotgun sequence.
ACCESSION   DAWACS010000099 DAWACS010000000
VERSION     DAWACS010000099.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2543_length_3897_cov_2.246486"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    272..281
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1253 a          623 c          958 g         1053 t
ORIGIN      
        1 cttataagtc aggttgtaaa aaaacttaca ccgattgcgg atgcaaagta tattgttata
       61 tatgttgaca atcatcagac agaatttagc ccgattttac ttgactatga caaaatgtat
      121 gaggcgatat acaatatcgc ggataatgcg attaaatatt cgccggaggg cggttttgtc
      181 catatcgacc ttacggcaga caatgactgt ctgacaataa aaattgagga caacggtccg
      241 ggaataccgg aggacgagcg tgacagaata tnnnnnnnnn natttgaacg tttttacaga
      301 cttgacgatt cgagagcgag agataccggc ggaacgggtc ttggacttgc tatcacaaaa
      361 gaggccgtac ttatgcataa cggtactata gacgttgcaa atgtgtcgga tataggcagt
      421 gtattcacaa tacgtttacc ttacaagatt actattggag gacagaatga aaagtaaaaa
      481 gggagtaata gctatcgttg cggttgcggt gctgacagtg gcggcggttg tcggaatact
      541 tataaaaaaa gattacggca gttatcgcta caacagtgaa ctgtatttct ttaatgaaac
      601 ggcgacttcg atagttatgg aaaatcgtga aattaaatat aaggacgata aggaacttgc
      661 ggagtctgta attgaggcac ttatgaaagg tcccgacaac agccgttata tgaaaattat
      721 tgaaaaaaat acgaagttgc tgtcgcttaa cgacgttgat tccggtaata ttgttgtaaa
      781 cttttcaggt gaatttttga caggcgacaa tacaaaagat gtgcttgcgg tttatgcagt
      841 tgtgaaatcg ttgtgtgcaa tcagcagtgt tgacagtgtt aaagtaattg ttgagggcaa
      901 ggatattgca acggcggacg gctcgattat agggtatttg cgaaatcagg acattaatct
      961 gccaaccgat acatataaca gcgaaacacg tgaaattgca ttgtattttc caaataaaga
     1021 cggcaacaag cttgttatgg aaacaagaac gattaaagtc accgaccaac aacctttggc
     1081 acaatatatt ataaatgaac ttatcaaagg tcccgaaaac aaggaactgt cagtgccgct
     1141 cagcaaggat accgtacttt tgagtgtgga aacttcggat aatatttgct ttgtaaattt
     1201 caaagctaat tttaccgata aaaattcagg tactgcggaa aaagagaaaa tgacgatata
     1261 ttcaatcgtt gattcgctta cagagcttga caatattcag cgtgttcaat tccttatgga
     1321 cggtaaaaaa gttgataatt tcggtaatat caatatcgga agtatgttcg gacgtgacgg
     1381 aagtataatt gccgaataaa tttagaatat gcagttgaca gtttggtaat aatatgatat
     1441 tatacaatca ataaagggga gtagcttgcg cattttatat gcgtaatatg atgtcgtcaa
     1501 tacacgttcg gaagaaccgt atcatattct acgaagcaag acttttattg cagataattt
     1561 tttgtgctgc aataaaaagt cttgcttttt tgtttgcggc ataagtgaga agatggagga
     1621 attgattatg aacccatatc aaatgaaaaa agaagatgtg cttaaaatgc tcgggactga
     1681 tgaaaatggt cttacgcaaa atcaagcaaa ggaaaatcag aaaaagtacg gcaaaaacga
     1741 acttgcggag ggtaaaaaga aaaatccgtt tatattgttt ttggagcagt acaaggattt
     1801 tcttgttata attcttatta ttgcggcgat tatatccggt gtgctcggcg atatagaaag
     1861 tgcgatcgta atatttgtag ttataacgat aaatgcgatt ttgggtactg tacagcatat
     1921 caaagcggaa cagtcgcttg acagtcttaa agaaatgtct gcaccgactg caaaggttat
     1981 ccgtgacggt gaaataaaag tcgttgaggg aaaagatgtg acagtaggcg atgttgtggt
     2041 cattgaagca ggtgattacg tctgttcgga cggacgtata attgaaaatg cgtccttgaa
     2101 ggttgatgaa agtgcgatga ccggcgagag tgagcctgtc gaaaaacagg aaaccgtact
     2161 tgacggcgaa aaaccgctcg gtgacagagt gaatatgctg tattcgggaa gttttgcgac
     2221 ttacggcaga gcgaaaatgg ttgtaacgtc agtcggtatg gaaaccgaaa tcggcaaaat
     2281 tgcgtcactg ttaaaaagta cgcaggaaaa gaaaacgccg ttgcaggaaa gccttgataa
     2341 tttcggtaaa aagctgtcgc ttatcataat cggaatatgc gttattgttt tgggtttgga
     2401 actgttccgc agtgacggta taaatttgac agtgtttacg gacgcatttg tgtttgcggt
     2461 tgcacttgcc gttgcggcaa ttccggaggc tttaagctcg attgttacga tagttttgtc
     2521 agtcggaaca cagaaacttg caaaagaaaa tgcgattata cgaaaattgc aggcagttga
     2581 gggattgggc agtgtttcta taatttgttc ggacaaaacg ggtacgctta cgcaaaataa
     2641 aatgactgtt caaaaaattt attttgacgg tcaaattatt gataaggacg atgaaatcaa
     2701 tgcacgtcaa ggtcagctta taatagacgg tgcattgtgt aacgactccg ttcaaaaaga
     2761 aggtcaggaa atcggtgatc cgacagaaat tgcacttgtg aatttttcgg aaaagcatga
     2821 tttgcctgtt gaaaaaatgc gtgaaaaata tcagcgtttg ggtgaaattc cttttgattc
     2881 agacagaaaa ttgatgagta ccgttcataa aatcggcgac aattataaaa tgctgacaaa
     2941 aggtgcggtt gacgttttgt cgggcagaat tgatgaagtc aaaactatgg acggaaaaag
     3001 accgtttact gccgaagatt tggcagagtt aaagaaagtt aatacggaat tttctcaaat
     3061 gggacttcgt gtacttgctg tgtgcgaacg tgacgttgat acagtcgaca taagcgttga
     3121 cgatgaaaag gactatatct tgctaggact tgttgcaatg caagacccgc ctagagaaga
     3181 aagtgcagag gcagtaagaa aatgtaaaac cgccggaata cgtccgataa tgataaccgg
     3241 cgaccacctt gtgaccgcgt cggcgattgc aagaaaaatc ggtatattgg atgataacgg
     3301 ccgtgcggta gagggcagag agattgaaaa tctgtcagat gaagaacttg atgaatttgt
     3361 ttcggacgtg tcggtttatg cgcgagtttc gcctgaacat aaaataagaa tagtcagcgc
     3421 atggcagaga aaaggctaca tcgtttcaat gaccggtgac ggagtgaatg acgcaccggc
     3481 acttaaacag gcggatatag gtgtggcaat gggtataacg ggcagtgaag tggcaaaaga
     3541 cgccgcgtca atggtgctta cggacgataa ttttgcaacg atagtcaagg caatcgaaaa
     3601 cggacgtaat ctgtacagaa atattcaacg tgcaattcaa ttcttgcttt cgggtaatac
     3661 ggcaggtata cttgcggtat tgtatgcgtc atttatggcg ttgcctgttc cgtttaaagc
     3721 ggtgcatctg ttgtttataa acttgcttac ggacagtttg cctgcgattg cactcggtgt
     3781 tgaaccgcac agcagtgatg ttatgaatga aaagccacga ccgaaaaatc aatctatact
     3841 tacaaaaaag gtacttacaa atatatgtgt tgagggcgtt gtgataggtg ttatgac
//
LOCUS       DAWACS010000100         3843 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2582_length_3843_cov_2.268215, whole genome shotgun sequence.
ACCESSION   DAWACS010000100 DAWACS010000000
VERSION     DAWACS010000100.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2582_length_3843_cov_2.268215"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2539..2548
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          970 a          804 c          725 g         1334 t
ORIGIN      
        1 gttctgtatg atgcttgaac aagaactgca tctgtatcct tatctgatgt caatgtaact
       61 gtatttgcag tttcatcaac gctcattgta acctttgcag gtatcggagc aggtgtcgga
      121 gctgcttgtg ttgttggaag aagttttctt ggttgatcag ctgttcctga atatgttagc
      181 atattatcaa ttcttgcaac tgaacctgtg taagaaccac ctttttcaga tgttactgct
      241 aacaatggaa gttctgtaac tgatgttgct gtattgcctg ttccaacgtg gtcgcaatca
      301 acttgtggtt tttctgtgaa atcttcatca gcttttgcaa cccaaattct aaatttatcg
      361 cttgaaacta taattgcaac tttgtaccat tgataagcct caacttgttg accgattgtt
      421 ataacattgc taccgctacc gattgtgtag cttgaggctg tgtcatcagt tgtaagtaca
      481 cccataacat tgttgtatgc accgttcttg tcagtaccag cttggctgtt atcctttaga
      541 atataaagtc taccggcacc gcctgccttg tttgagccaa gattaacagc gaatgtcatt
      601 acagatgatt ttccgtctgc taatgccaaa gtatttgcag cagaaactac aggacctcta
      661 ccggcacctg aataaggacc acctgcaagt gtaagaactt tatcgccagc tgcccaatct
      721 tctactgaaa cagttgttgc tgtatcgccg cctgttctat tacccatagc aatagataga
      781 ccgtctacat ctgtaattgt tgtagcagtt tcgcccatcg ctgttgatgc aagtgctttg
      841 ctgaaagtat tattaactac cggtgtaact ccatctggag ctgaaaggcc aggagcctgt
      901 gtcttaacgt ccggaaccgg cataactgtt ggaactacaa cagttgcatc acctctaaga
      961 ccatctgttg taagtgtttc cggagcacct tggtaaatat gtaagttgtc aagtgcaact
     1021 ataccactac caccatcacc tctgtttgag caaacatata ttgaattaat ttcatttgtt
     1081 gagtttgtat attctacacc gtcaacaagt ttattacctg acttatcgat tactgttaca
     1141 gtagtcttat cattattaac tgcagcttgt actcttaccc atttagtatc tgcaacctcg
     1201 actgcacttt caggataagc tactaattct gatgttgaat catcattaat tacccattta
     1261 tctgtaagat atggtctaag gtcagttttc atagcagcgt tagctactga cgcacctgaa
     1321 cctgaattat actttgtacc ttgttgtgca tttactgtat ctataatacc tgcaccatct
     1381 cttgtatcct tagtgtttgt atcatttgta aatgcaagtt ggtttgagtg catgattctg
     1441 ctcataccat taccacgagt catcattgta tcatactcaa tagtatattg acctgaaata
     1501 gtttgaccca aagaatatgt cgacttacgg ttaccgccgc cgctaccttg gaattttaaa
     1561 tattggtcag tttcaccgtc atcaatggta actgtcggaa caatgtcacc tttatctgct
     1621 ccatccggag caacccaacc atctgctgtt gagccatcaa gagtgactaa atctgttgcc
     1681 gcgcttgctg ttactgtcaa gcctgcgaaa gcagatgctg acatagcagc ggcgcaaagt
     1741 aatgaaatta gttttttact tttcacttct tctctccctt tctttcctta acttgtttcc
     1801 aagtattgtt taatttagca ggtacaggat gttgatttaa gtaaatctgc acaatcctaa
     1861 cccatctata tggtatatat tgtatcatat tttttttgta tgtgcaatat tattaagaaa
     1921 ctttgaatga aaatattgca caaaaaagca catcattttt tattcaaaat gcaaaattca
     1981 agttgtacat tttttatttt tgtagattat ttttaccaac aaaagaccgc tttcgcggtc
     2041 ttttaagcgt tattatttta taactgtgaa attgctgata ccgattgctc tgtcctccgg
     2101 attcggaact gagcttgctg acgcaaaatc aacactcgca atatctcctg cattgtctgc
     2161 aagagttgtt gttgcagatt ttgtaccgtc tgttactgtt acaacctttg tttcaggatc
     2221 aactgtaaca gttactgtgg tatatttatt ttgttcaaca gccgccgctt caagtccgtc
     2281 tgttgcggta cttccggcat acaaagccgc ccatttatag gtcattgaga atattgttgt
     2341 gcctgatgaa ttcttgaagt taagagttct aagctgaccg caagtcttac tctttgacca
     2401 cacgtgatca aacttaactg ttgttgtttt atcaagggtt acaggtgagc cgaatgtaaa
     2461 tgtctttgtc tgtgatgagc cagggaaaac tattgctgtt ttaccgctga tagtcataaa
     2521 ctgacctgta taattagtnn nnnnnnnnct gttacaacct tagtttcagg gtcaatcgtt
     2581 acagttactg ttgtatattc tgtcttttta tcaggcaatg tagcagcttc taatcctgct
     2641 gttgcagctg taccggcata caatgtaccc catgaataac acattgagaa tattgttgta
     2701 cctgaagaat tcttgaagtt aagagtcttt agctgtcctg ctccccattt atttttcgag
     2761 tcaggtgccc atgtatgatc aaatttaact gttgttgctt tatcaagagt tattcttgat
     2821 ccgaatgtat atgtctgagt ttgacctaaa ccagggaata ctatagcagt ctttccgcca
     2881 agagtcataa atttacctgt atagttagtt gaaccctctg ccatatcttc gtaatattca
     2941 taaccgaata ctgtcaatgt tgccgtcttt gttacgccgt ttagtgtacc cttaattgta
     3001 acgttctttg tcaatgaatc gcccattgag aaaccgtcac cgattgtaac aacaccgttt
     3061 gcgtctattg tcacacctgt ttcatcacaa gaccatgtaa catctgtgtt tgtaacctta
     3121 tccataggtg ttccgactga gtctgttgca ttcttaattg aaacagttgt tgttgagccg
     3181 gttgtcgact taatacttgc aggtgatagt gaaagttcaa aatctgtaat atccgcaact
     3241 gtcggtgcgt ttacaaaatt agctacaatt cttgagtcac cgcgaagtct gaatgtatat
     3301 tcatcatcca ttgaaagtgt tgttccgctt tcagtcttcc aaccacggaa tacatatcct
     3361 tcatttgctg ttgcctttaa tgttacaaca ttgtttagag gaagtgtttc cattgtatac
     3421 tcactgtccg gaatcggtgt tgcagtcggt gcagctgtcg gagcaggtgc tacactgctt
     3481 actgtcaacg gagtcatttt ttgaagcgaa tcccaaagca ttaccttact tcctgttgta
     3541 actgtgtaat cttgtgtacc tgcctcttca aatgttacag gagttgttgt aaccttttta
     3601 attgttccgt tttctgtata ttgtgcctca acaagaactg ctgtcaatgc ctttgttgca
     3661 gttacaacag ctttatcggc agtaactgtc aatgtaacgt caccgtctgc caaagtattt
     3721 actgttgctg tttcattatc tttagcctct gcagttgctg tcggagctgc tgttgcagtt
     3781 gcctctgtag gttctgttgt tgctgtaggt tctgttgtcg ctgtaggttc tgttgtcact
     3841 gta
//
LOCUS       DAWACS010000101         3825 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2599_length_3825_cov_2.111406, whole genome shotgun sequence.
ACCESSION   DAWACS010000101 DAWACS010000000
VERSION     DAWACS010000101.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2599_length_3825_cov_2.111406"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    555..564
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2890..2899
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1241 a          588 c          932 g         1044 t
ORIGIN      
        1 ggataagatt aacgcgtaca tgactcttta taccgcactt gtaacgcttt gtaaggcggc
       61 tgcaccgatg attccgttta tgacagagga tatttatcaa aatcttgtaa gaaatattga
      121 taagtccgct ccggaaagta ttcacttgtg cgacttccct gaatatgacg aaaaacttat
      181 tgataaagaa cttgaaaatg atatggaaga agttcttgat atagttgtgc ttggtcgtgc
      241 gtgccgtaat actgcaaata ttaagaaccg tcagccaatc ggtatgatgt atgttaaagc
      301 accggaggca cttagtgatt tctatattga cattattgag gacgaactta acgttaaaaa
      361 ggttgaattt acagatgacg ttgccgcatt tactgcatac agctttaaac cgcagttaaa
      421 gactcttggc agacgtttcg gtaagaatat caacgcagta agagaaatcc ttgctaatat
      481 tgacggcagc aaggctatgg ctgaacttaa ggaaactggc tcactaaaga ttactgttga
      541 cggaaatgaa gaatnnnnnn nnnngctcat aaggaaggct ttgaatcaaa cagcgattac
      601 ggtattactg ttgtgcttga tacaaacctt tctgaagaac ttatcgaaga aggatttgtt
      661 cgtgaagtga tttcaaaaat tcagacaatg agaaaagatt ctgattttga agttatggac
      721 cacatcaaag taagctgtga gggtaacgat aagattgcta agattatcac tgataatgaa
      781 agcactatca aagacgaaac tcttgcagat gaagttgata caaattctgc cgacggtaac
      841 acaaaagaat ggaacatcaa cggtgaaaag gtaacacttg gtgttaagaa actctaattt
      901 ggattgacag aaaatcatat acattatata ataatgtata tgattttttt gtggagaaaa
      961 acagatgaaa agcaacaggt ttttaaagaa aggttacacg actgggagtt gtgctgcggc
     1021 ggcggcaaaa gcggcggtta tgactatgtt aaatgacgaa atagtcataa caactcaaat
     1081 tacacttccc aaaggtgaga gcataagtat tgacataacc gacacgcaga ttgacggtga
     1141 cagtgtcact tgcacagtta aaaagtacag cggtgacgat cccgacataa ccaacggtat
     1201 acttgtttgt gcgactgtca gaaaaaatag tggcggtata aaaattgacg gcggtgtggg
     1261 cgtaggtcgc gtgactcgta acggacttga ccaacctgtc ggaaatgccg cgataaacag
     1321 tgttccgcgt caaatgatta gaaacagtat aaatgagatt tgcggtgatt atgacggcgg
     1381 ttttgatgtg attatttccg tgccgaacgg cgaagaaatc gcgaaaaaga catttaattc
     1441 aagactcggt attgagggtg gaatttcgat tttgggtaca agcggaattg ttgaacctat
     1501 gagcgaaaag gcacttttgg acacgatttt ccttgaactc aatacacgaa aaagtgccgg
     1561 cgacagtatt gcggtgcttg tgccgggcaa ttacggcgag gattttgcta aaaaaacttt
     1621 tggtataaaa aataccgttc agtgcagtaa ttatattggt gacgcgatag attatgcgtc
     1681 cgatttgggt ttttcggata tactgataat ttcgcatatg ggtaaacttg ttaagcttgg
     1741 cagcggaatt atgaatactc attcaaaata tgcggacggc agaatggaaa cgcttgcgtt
     1801 atgtgcggct ttggccggtg tggataattt tgcggatatt ctcgattgtg tgacgaccga
     1861 tgaggcttat gaaattattg aggacactaa aactattgat atattgatga aaagaattga
     1921 taaataccta aagcacagaa gtgatgtcaa tatcggtgca ataatgtttt tgaataaaca
     1981 gggaattatc ggaaaaactg ctgatgtcga cggtattttg gaaaggatat aaaatggtac
     2041 attttgttgg agcgggcagc ggtgcagcgg atcttataac tatacgggga aaaaaacttt
     2101 tggaaaatgc agatattatt atttatgccg gttcgcttgt taatcccgag cttttggaat
     2161 atgcaaaaga cagtgctgaa atttataaca gtgcaaaaat gaatcttgat gaagttattg
     2221 acgtgatgaa acagggggac actaaaaaca ttgtgcgttt gcatacgggt gatccgtgtg
     2281 tttacggtgc aattcgtgaa caaatggaca ggcttgataa acttggaatt gaatatgatg
     2341 tttgccccgg tgtaagctcg ttttgcggtg cggcggcggc tttgaaggcg gaatatacgt
     2401 tgcctgatgt atcgcaaacc gtcataataa cgcgtatgga gggcagaact cctgtgcctc
     2461 cgaaagaaaa aatacatttg cttgcgtcac ataatgcgac aatggtgata tttttatcaa
     2521 caggtttatt aaaggaattg tcggctgaac ttgtaaaagg cggttacagt gaaaatacac
     2581 ctgcggcgat agtttacaag gctacttgga aagacgaaaa agttatgcgt tgcaaagtgg
     2641 ggacactaaa taaaacagca gagaaaaacg gtataaaaaa gaccgcactt ataacagtcg
     2701 gtgattttct cggcgatgat tattcactgt cgaaacttta cgataagaca tttacaaccg
     2761 aattcagaaa gggcgtgacg gaatgaaaat tgcgtcaata gcttttacgg aaaacggtgc
     2821 gaaaatcgta aaaatgctcg tgcgtgaaat ggacgtaaag ggctacgttt ttgaaaaata
     2881 taagactgan nnnnnnnnna tttaataatg tttcgtcact tgtcagagat attttcaaga
     2941 aatataatgc gattgtgttt gtcggcgcgt gcggaattgc ggtgcgttcg atagctccgt
     3001 atgtcaagga taaagccaaa gatccggcag ttgtggtggt tgatgaaaaa ggaaattttg
     3061 cgataccgat tttgtccgga catataggcg gtgcgaatga tttggcggag aaaatcgccg
     3121 cattgacaag cggagtggcg gttataacta cggcaaccga tattaacaaa aaattctctg
     3181 ttgacacatt tgcggtaaga aataatttgc atatcgggga cactaaactt ataaaagaaa
     3241 tatcgtcacg gattttgaac gacaaaaaag tcggattgta taccgattac gaattaaaaa
     3301 acgttcccga ttgttttgaa gaaagcaatg aagtcggaat ttgcataagt gatgacgata
     3361 aaaagccgtt tagaactacg cttaatttaa tgccgaaaaa tgttgtactc ggaatcggtt
     3421 gcagaaaagg ctgcgaaact gtcgaagaaa gtatattggc atttttaaaa gtgaacggag
     3481 tgtcggttta ctcgcttttt gcggttgcga cggtggatat aaagaaaaat gaaaagggca
     3541 ttgttgaatt ttgtgaaaaa tttgatatac cgcttttgac gtttccggct gaaactttgg
     3601 cggcggttga aggagaattt acaacgtccg aatttgtgaa aaagactgtc ggagtcgaca
     3661 atgtgtgcga aagagcggtt tgtgcggtcg gtgcgaaaat aacagaaaaa aagacggctt
     3721 taaacggtac ggctttggca ctcggaagaa taaatactga aattgatttt ttgaaaggtt
     3781 aagtttgaat gaaaatttat gttgttggaa taggcgcagg aaatg
//
LOCUS       DAWACS010000102         3788 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2636_length_3788_cov_1.652023, whole genome shotgun sequence.
ACCESSION   DAWACS010000102 DAWACS010000000
VERSION     DAWACS010000102.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2636_length_3788_cov_1.652023"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2283..2292
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3383..3392
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1067 a          883 c          589 g         1229 t
ORIGIN      
        1 tacatacaac tgccagtccc ttttcagata gtatttaaag ccttttttat ctctctcttt
       61 taacttttta ccctttttca ttaaacagct ttaccccctc tttttacctt aatttgttac
      121 tatgcaatat aaatactatc attttttatg caatatgtca atcaaattac gttttttata
      181 taaaaaagtc gtctttttat acaaaacaaa aaggacgttt taaaaacgtc ctttttgagt
      241 gttgttgttt attttttaat taatcgactg ccattccaca cataaaccgc agatttacct
      301 tgcggcagat ttaccgaaac gatctctgcc gtatttgcgt ttacttcttt tgacacgttt
      361 tgtacactgt ttatgacatt gttttcgtca taaccgatta caataacact tacctttttc
      421 gtactgtcgg tattgttttc aattcgtgtt ttgacattct tttcatcact tatagtttgg
      481 atattgactt cttccttact gcttgaagaa tgtttatata cgtcaaccga cggaatagct
      541 tttccgtcaa aatcgaaaag tgttgtgttt tcgacaacgt taacgtctgt cttatcgtca
      601 ctttccttta cagcccaacc cgacaaagac gcatttttat tatctttatc ttcaacgtga
      661 atcatacaag ggtcccaata aagcacaccc agacatctgt tatcgtcaac gctttttaat
      721 tcattgaaaa gttctgccat aaaattttta tgtcctgtca ttgtcggcgg ataaatgtcc
      781 ttatacacat ctgaatcctt taactgtccc gcataaccgt cttttcttgt ttcgttccaa
      841 ttaaaacctg tttccataat cataatgtct ttgtcgtact tctttgaaat ttcattacaa
      901 aattccttac aggcttccgc attgttgtcc gtccatgcag gataatatga cgcaccgatt
      961 acgtcatact ttgcaccgag tttgtcaagt tcatcaaaga aatatgtgaa tttgtttgac
     1021 tttgaaccgt cgtcaaggtg aattataacc tttgagtcct ccgacaccgc ctttaccgcg
     1081 tcatatcctg cattgataaa cttaacaaga ccttcccaat cttttgggca ttttatatct
     1141 tcatctgcgt ttttatcgtc accgaacaca agttcaaatc tatctctgtt catacttgca
     1201 tcatatgtgt tgccgaacgg aaacaacatt ccgccgcgaa tttcattgcc aagcgaaaca
     1261 tattccggaa cagtcccctg tgcctttaac tttgacataa tatcatatgt atagttgtat
     1321 actatttccg caaatttatc ttgaatttcc tctctttgtt cacttgtcat tgagtttaga
     1381 aagtccgcat tcttaacgtc atagccaagc tcgtctttaa tctgcttaac ccattcggac
     1441 ggaataatct gtcttgagcc gttcgaccaa taatcggaat aattaaacgt gaactgtatt
     1501 cccatacctg cgtcctttgc acgtttggca agcttcagac aatcctcctc atcctgaaaa
     1561 ccgctcggga gataatacac gccgtcacct gtacccttgc ccggtgtatt gctaagacgt
     1621 attctcgcca tattcatacc gttctgtgac aaaaattcaa acgggtcaac cttgtttccg
     1681 tcattgtctt tatattcacc gccaaggctt tcgatataat caacttcggt catatcgcca
     1741 ccttgtataa agccgttttg cttgtccgtc ttaacaaggt caacgtccga tacttcaatc
     1801 acgccgtttc cgtcagtacc aacgccgatt gtgcatttac cgttttcaac ttcaattcct
     1861 cttacggtaa ccttaacacc gtcagccgaa acaggtaaaa cagtacttgc cgcagtgtat
     1921 ccgtccgaca ctccgtacaa atacgacaca ccctcctccg aagtattttt acatactgcc
     1981 gtaaggtcat aatatccgtt ttcaagatta gttacttcaa cgcttttacc gcccgtaacg
     2041 tcaccgctga aatcaatgct ttgtgcatat gccacagtct gtaaaacagc aaccgccgct
     2101 ataaccatta atatcttttt catacagcac actccttttt attgttttaa tatttttttg
     2161 cttcttattg taaacattat cgttgtacag cttgccgcaa cgacatcact taccggctcc
     2221 gccaaaagca atccccatac tcccatattt gcgatattcg gaagtctaag tgcaagcgga
     2281 aannnnnnnn nnagttgagt ttatacctat atctataagc tcttggtcat ttgtgaacat
     2341 cttcaaaaac gctgtcggga acacttcgac aagcgcaacc gcaaaaagag agaaacatat
     2401 actcaccgta aattgaagtt taaacgcttt tttcactctg tcaaaacgct ttgcaccgta
     2461 attatatccg ataacaggct gtgcgccttg cgaaaaaccg ttaagcggca tccatataag
     2521 ctgtgtcaaa ctgaacagta tcgacatcag tgcaacatac aggtcatttc cgtatttaag
     2581 cataccgttg ttgaatgtaa gctgaattaa acattccgtt gcctgcatta taaacggtga
     2641 cacaccgagc gacaaaaccg aagtaacgat ttttccgtcc aaaacaagat ttttaagtct
     2701 gattttcaaa atggtctttt tacccgtcaa aaatttaagc acccacaaac acgatacacc
     2761 ttgcgaaatt atcgttgcaa gcgccgcacc ctttacgccc attccgaaca catatataaa
     2821 aatcgggtca agcacaatat tcagcaccgc acctatgcaa acggtcatca tacttgtttt
     2881 tgcaaagcct tgatttgtta taaacaaatt cagtcccaac gcaatctgta caaatatcgt
     2941 accgacaaga tatatgctta tataatccga cgcatacggc aaagttgcct cacttgcgcc
     3001 gaatttcatc aatatctgat ttttcgtaag cataaacaca accgccagca ctaccgaaaa
     3061 tattataagc agcattgtac agttgccgag tattttctct gctttgtcgt tgtctttttc
     3121 gcccatgctt accgccgctc ttgatgcacc gcccatacct gcaagcattg caaatgccga
     3181 aacaagcaat attataggaa acgtaacacc caagcctgca agcgaaagtg agcctatctc
     3241 cggtattcgt ccgacataaa ttctgtcaac tatgttataa agcaagttta caagctgtgc
     3301 cataacagtc ggtatcgcaa gcttaaacac aagttttcct atcggtgcgg tttcaagctg
     3361 attattttta tccattaaat acnnnnnnnn nngattatta ctttctttta taagattaac
     3421 aaccgtcatg gctatagcct tattattaag caagtcaaat tttgtttcgt tatcagatag
     3481 aatcattatt acacgtataa ttaaaactat ctcgaaaggg gggtcgtttt aattatttct
     3541 cctttcctca aaattgttat atttgcagga gtagctacct gcgatataat aaagacaagc
     3601 actgtggatt tgtatctatt gttttacagc tcagactggt tcgaggtgac tcaaaccaca
     3661 gtggtttgtc tgaaatggta attagttagt taacgctctg acgtttctat ttgcgtgaat
     3721 acattggcag accgtctgtg gaaaacagca gtgcaaaaat atcaaaagga ggaacccatg
     3781 tattacat
//
LOCUS       DAWACS010000103         3787 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2637_length_3787_cov_2.016613, whole genome shotgun sequence.
ACCESSION   DAWACS010000103 DAWACS010000000
VERSION     DAWACS010000103.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2637_length_3787_cov_2.016613"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          989 a          897 c          603 g         1298 t
ORIGIN      
        1 acatttctcg gattaaatct cttgattgat tcaatcatct ttgatgtagg ctggtacaat
       61 actctgtaat tttcactgtc ttggctgtaa tcaacaaagt aaacattacc tctcgactta
      121 tcgcccgtaa cgtcaaaagt tttgcttact gttacatttt tatagtcgtg attatgctcg
      181 ttgtcattga tattcgcaca aatatttatt tctttaaagt caaaactcgc aattcttttt
      241 cttgctttct ttgacataat cttatcaata tcaatttcac tgtttgtaag tatatattcg
      301 tactcaatat tttgcatact cataagcaaa tacgcaccgt accacgcaaa tgctataagc
      361 acaagtccga tactaaatga aaatgaaccg aattgagtgc ctgaaagtgc agtagccgtt
      421 gcaaaaataa gtaatagcaa agccagcgat acaataatcg ccgcaaaaat tataactgcg
      481 ataatcgcct tttgtccgcc tgtctttttc ttctttagta tgtattccaa atatatatca
      541 ttcatcatag caatctgtta cctttcttat atttaattca ttttcttcaa cttatcatac
      601 agatttttca atgcacgtcc gcgatgactg attgaatttt tttcttcgtc actgctttcg
      661 gcaaatgttt taccgagttc gtcagacaaa aatatcggat catatccaaa tccgttttca
      721 cccttaggct cttttgttat cgtgcctttt acttcaccct cagcggttat ttccgtaccg
      781 tcaggcatta taaacgctac cgacgtcaca aactttgctc tgcggttttc gttgtcgccg
      841 agttccgtta aaagtttgtt gattctgtcc tcgtccgttg cattgtcacc ggcatatctt
      901 gcacttctga cacccggcgc accgcctagt gcctcaacgc atatgcctga atcatccgca
      961 aggataggat aatcgcaaac catagcaacc gctcttgcct taataagtgc gttttttaca
     1021 aacgtgtcac ccgtttcttc cacatcaaca tcaattccaa gctcttgttg tgatacgatt
     1081 tcaatgtcaa gcggtgcaag aatttcttgc atttctctta tttttccttt gttttttgtt
     1141 gcagctatta ccttcataag actgcctccg taatttaatt atttctctaa atattctact
     1201 acatatagcg ttttttttca aggtctaaat agaaaattac acaatttaag tacaaaaaat
     1261 gcactccgta aagagtgcat tttcatgact gtcgccatta tttttacttg cttagttctt
     1321 catctattgc agcagctgcg gctttaccag cacccattgc aagaataact gttgcggcac
     1381 ctgttacaac gtcaccgccg gcgtatacat ggtcttttga agttttacct gtttcctcat
     1441 cagcaatgat acaacctctc ttatttgtat caagaccttt tgttgtcttt ctgataagtg
     1501 ggttcggagt ttgaccgata gctacaacta ctgtatctac gtcaataatt tcttcactgc
     1561 cctcgatagg aacaggacgt cttctgccac tgtcgtcagg ctcgccaagt tccatcttga
     1621 taacttccat accgtttacc caaccgtcct cgttgccaag aatctttgtc ggattttgaa
     1681 gaagtttaaa ttcgatacct tcttcttctg cgtggaatac ttcttctgct cttgcaggaa
     1741 gttcttcctt actacgtctg taaacgatat atacgttttc agcgccaaga cgctttgccg
     1801 atcttgcagc gtccatagca acgttaccgc cgccaagaac cgcaacattt ttacctacat
     1861 atacaggtgt atcgtattca gggaacttat aacccttcat aagattaatt cttgttaaga
     1921 attcatttgc actgtataca ccgttaaggc tttcgccctc gatacccata aagtgaggca
     1981 gacctgcacc tgagccgata tataccgcat caaaattcat ttcgtcaaac aattcgtcaa
     2041 cagtaagtgt cttaccgata actacgttag tttcgatgtc aacgcccatt tcctttaggt
     2101 tagagatttc cttttgtact atatcctttg gaagtctgaa ttcaggaata ccgtacatca
     2161 aaacaccgcc tgctgtatgg aatgcctcgt aaattgttac ttcgtaaccc tttttagcaa
     2221 ggtcgcccgc aactgtaagt cctgaaggac cgcttcctac aactgcaact ctcttaccgt
     2281 ttgaaacagg cttttcaatt tcttccttga cattcttcat atgccagtcg gcaacaaatc
     2341 tttcaagtct gccgataccg acgctttcgc ccttgatacc tcttacacac tttgattcac
     2401 actgtttttc ctgcggacat actctgccgc aaacagcagg aagtgcattt gtttccttaa
     2461 tcttcttata agcgttttca aattcaccct ttgcaacaaa cgcgataaat tcagggattt
     2521 taacgcttac aggacagcct tgcatacaag gcttatgttt acagttaaga catctctgtg
     2581 cctcgtctat tgccatttcc tctgtataac ctgttgtaac ttcaaggaaa ttcttatttc
     2641 ttacattagg acattgctcc ggcatcggat ttttgtccat tctcatattt ggcatattaa
     2701 aaatcctccc ttaatcagtc ttgatgattg gttctgccta ttctgcagtg accttctttg
     2761 catgattttt cttcataacc cttaaacatt ctgccacgtt tcatagcagt atcccagtca
     2821 atcttgtgac cgtcaaagtc aggaccgtca acacaagcga actttgtttc gccgccgacg
     2881 attacacgac agccgccgca catacctgta ccgtcaatca taacagggtt cattgaaact
     2941 gttgtaggaa tgttgtattg ttctgtcagc ttacagacaa atttcatcat tacaagcgga
     3001 ccgattgcga ttacttcgtc aaacttttcg cctgcttcga tttctttttt tagcatatct
     3061 gtaacaaagc cttgatttcc gtttgaaccg tcgtctgttg caacgataag cttgtttgaa
     3121 acttccttta gttcatcttc aagaataact aaatccttgt ttctaaaacc tgtgataacc
     3181 gttacatcgg caccaagatt atgaagtttc ttagcctgcg gataagcgat agctgtacca
     3241 agaccgccgc cgataaccgc gactttcttt agaccttcaa gcggtgtagg tgttcccaaa
     3301 ggtccggcaa agtcaagaat ttcttctcct tcttcaacct gtgcaaggtc ctttgttgtc
     3361 ttacctacga tttgtacgat aattgtaact gttccctttt ctctgtcaaa atctgcgatt
     3421 gtaaacggaa ctctttcgcc gtattcatca attctcaaaa tgatgaactg tcccggctct
     3481 gctttcttcg caaccaatgg agcttcaacc tccattaaaa agatttgcgg attaagcaca
     3541 ttctttttaa ctactttgta agccattata tcacctctta aatttagttc aatacattaa
     3601 aatattaata catatatgtt atcatatcat aactttactg ttttttcaat gattttacga
     3661 aagtttttcg ataaaagctc tcatatcttc tgccatttcg tctttaaact ccattatttg
     3721 acctgttatc ggatgcgtaa agcaaagata gcacgaatgt agcatctgtc tttttacaat
     3781 attatgg
//
LOCUS       DAWACS010000104         3774 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2642_length_3774_cov_2.210272, whole genome shotgun sequence.
ACCESSION   DAWACS010000104 DAWACS010000000
VERSION     DAWACS010000104.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2642_length_3774_cov_2.210272"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    454..463
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          924 a          839 c          630 g         1371 t
ORIGIN      
        1 cgtccgaata tcttgcggga ggttgagtaa agtgttgctt ttcctccgaa cttttaagtg
       61 caacggtttg attttcttca aggttaggaa gcattttttc tttcttttcc ttgttgtccg
      121 ttgactcaac gtacaccgtc atataaccct taaactttat gtttgaaccg ctcgccttaa
      181 atgtatgacc gtttgcgtca atagttacct gcattgtgtc atataccgcg cttgtcatct
      241 gacttgcggc aaatcgttcc caaataagct tataaagtct gtattggtcg tttgtaagtt
      301 tatccttaat atcaatcggc tttatgttta ttgaagtcgg tctgatagcc tcatgggcgt
      361 cctgtgcatt cttttttgtc ttgtattgct ttgcctttac atattcgctt ccgtaatttt
      421 cggtcaaata tgcaaccgcc tctttttgtg cgtnnnnnnn nnntctcata tacgttataa
      481 gacctattgt tcctatcttt ccgccgaggt taataccttc gtaaagagtt tgtgcaatct
      541 gcattgtttt tgccgcttgg aaattatact ttcttgatgc gtcctgctgc agagtacttg
      601 tcgtaaacgg cggttgagga ttacgctttt gctgacctct tttaaccttt tctataagaa
      661 aatcggcatt tgctattgcg tccgaaactt tctttgtttc ttcggcattg tttagcttta
      721 cttctttgcc cttttcaccg taatattttg cattaaaatc ctttttactt ttcggatcat
      781 tcaaaaccgt ttcgattgac caatattcct gaggagtaaa gttttcgatt tcttcttccc
      841 tgtcacatat aagctttgtc gcaacagact gtacacgacc ggcactaagt cctcttttaa
      901 ccttttccca aagaatagga ctgattgtat aacccacaat tctgtcaagc acacgtcttg
      961 cctgttgtgc gtcaacaagg tccatatcaa tctttctcgg ctgttttatt gccgccttaa
     1021 cggctgtttt tgtaatttcg ttaaacgtta cacggcaatt tgactcgggg tctattttaa
     1081 gtgcctccgc caaatgccag cttattgcct cgccctcgcg gtccgggtca gttgcgagat
     1141 aaactacatc tgctttttta gcctcttttt tcagttgtgt aagcagctgt cctttaccgc
     1201 gtattgttat atatttcggt gtgtaatcat tttcaaagtc tatacccatt tgacttttcg
     1261 gaaggtcgat tatatgcccc atagacgcca taactgtata gtccttgcca agatattttt
     1321 ttattgtccc cgctttcgcc ggtgactcca ctattaacag ctttttcgtt gccatataat
     1381 ttcctccgta tctataattt cagttgatat ttacttccgg gaagtttctt aaccaatccc
     1441 ttgacttcaa gcattataag ctttgtattt atttcgctta cgtttctttt cagttccctt
     1501 gaaagttcat ctatctgcat atcgcttttc tttaatatat ctattatctg tttttcaaat
     1561 tcatttagtt tgctgtattt actgtcatca acagtttttt gtatcggctc actttttacc
     1621 tttttaggta taactttttc ggcatacgga tattcgctta ttatatccgc tgcgccgttt
     1681 atcagttttg caccctgttg aatgattttg ttcgttccca agaaatttgt atctgtaata
     1741 tttctcggta ccgcaaatac atctctgtca ttttcaagtg catattttgc tgttataagc
     1801 gcgccgctct tttccggtgc ttccgttacg agtattccgc gtgacagtcc cgctataatt
     1861 ctgttccttg caggaaaatg atttcttata ggcggtgtac cgggtgcgta ttctgatatt
     1921 atacaaccct tttcggtaat tgctttgaca agttcaacat tttccgacgg atacactata
     1981 tcaagtccgc tgccgacaac accgaccgcc actccgccga catcaagtgt tgcccacgca
     2041 ccgaccgtat caatacctct tgccagtccg ccgacagtca tcgcaccgta ttgtgccaat
     2101 tcgcgacata tcctgtccgt cacaacacgt ccgtattccg acgaatttcg tgtacccaca
     2161 acgcctattg taagcacctt atcaagctcc aacaccttgc cctgtacata caagacatac
     2221 ggcggacttg atatattctt taatatttgc ggatagcttt cactgtcgta aaccaatatt
     2281 ttctgattta attttgcggt ttgttcaagt attttttctg ctttggataa atccttgtct
     2341 gcaagacttg attttatttt gtcactcaaa ccgtatatat tattgaagtt ttttgaaaaa
     2401 tatatttctt caacagtttt aaaatactct aaaagtactt taatttttga tggcgtaaca
     2461 ccgtatttag ttgtaagcca taaccaataa agtatatgtt ccatcaatcc accacctaaa
     2521 atacatctgt tacattacta caaatctcaa aaattgtcaa gacctttggc gaacaagttc
     2581 gtacattaat atcgccgccg caactcccgc atttagcgat tcggctttcc ccaaaatcgg
     2641 aattttaacg cattgacaca ttttctgaac atcttccgat attccgtttg cctcatttcc
     2701 tactattata atagtaggtt ttttcatatc cgcactgcgg taatctattg tattgtcacc
     2761 aagggcaccg ccgataagct gaaaaccgct gtttttgtat tcgctgagtt tttcatatga
     2821 cacacctgtt acaactctca tattgaaaaa tgaacccata cttgaacgca ccgttttcgg
     2881 actgtataaa tcaacgcacc catccgacaa caacactccg tcaaatcctg ccgcatcagc
     2941 cgttctgatt atcgttccga gattacccgg gtcatttata cagtcgcaat atatatacga
     3001 tttttccgtg ttgggtgtaa aatcggtttc atcttcgatt ttcacaaccg caagtatgcc
     3061 ctgcggtgct tttgtatcgc acattttcat aaatacgtcg tcttgtacct tgtataatga
     3121 aatatctttt ggatatttga atttttcgtt ttcaaaaaaa ctgtccgata catatagtgc
     3181 cgttatctcc ctttcggaat ttaatgcgtc cgataccgat tttattcctt ctattgtata
     3241 ctcaccgtat ttctgccttg attttttctg cgacagcgat tttacatatt tgcatttatt
     3301 gtttgatgaa gaagtaatct ctaacataaa tcatttcctt atctattcta tataagtatt
     3361 ttctactaat tatataccct ttttttgaaa ttggcaacaa ttaatttaag taacaaaaaa
     3421 aattcgtgca acgtattata ccataagaac ttttaaggag atgattttag tggaaacaaa
     3481 tgtactgttc gcattgtcgc tgacggttat ggcaggactg gcaaccggaa tcggcagtat
     3541 aatagcattg tttgcaaaaa ccacaaatac aaagtttctc gccagctcgc tcggattttc
     3601 agccggagta atgatatacg tttcaatgat tgaaattttt cagaagtcac gtacatatat
     3661 tgcgtcagct acaaatgaca ctgtgggata ttatatcgcg gtagtatcat tctttgtcgg
     3721 aatacttctt atcggactta tagactactt tgtgccgtca acagagggtg atat
//
LOCUS       DAWACS010000105         3774 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2643_length_3774_cov_2.165098, whole genome shotgun sequence.
ACCESSION   DAWACS010000105 DAWACS010000000
VERSION     DAWACS010000105.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2643_length_3774_cov_2.165098"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    951..960
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3314..3323
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1005 a          958 c          617 g         1174 t
ORIGIN      
        1 cttcatccgc atactgacgc tttgaacatc cttcaacaac cttaaatccg cgccaatgct
       61 taccttcgtt tatcgtcttc gcctgcgcgt atgcaaacac atcctccgcc catttggaga
      121 ggtcggcggc aatcgtcaga acacccgcta tttcctcatc ggtcagttct gcaggttcct
      181 tgaattcgtg tttcgcagtt tcaagcataa actctgccct ctttcggcag gtggctctaa
      241 gcttacagaa ccgacaatga cttccggctt tgaactcacc ctcgcctttc gctgcaagct
      301 gcgcgattgg tttgagtgtg ttttccgccc atgataacag ctcgtcaaca gacatctccc
      361 actcggaaat actgtcaagt ctcggctgaa caatcgccat cttaaccatt tttatatcat
      421 acagataatc ataaagattt aacgcaccca aagcatacag catcatctgc ggattatgct
      481 ctgccgatac agccacacct ttgccgtact tgaaatcaat tacctgtata acatcatcag
      541 ctacaatgac aaggtcgcct gtgccaaagc cgtccttgac atagtttgaa aagtcaagtc
      601 tctgctccac aagaacgaca gcgtcaggac acgactttct gattgtttca attttgtccg
      661 taacataata cgcatagctg tctgtgtatc ggtcaatctc ctctgcgtta aagtttccgg
      721 tagaaggcat tggaatatcc cgttcaccta aataccacct gaccttatac tcagcgacct
      781 cgtgagcgac agagccttcc tctgcataca cagttgtttc atcgggcagt tccttctcaa
      841 gcatagccga aggcggacac gcaagccagc gatgcgccga tgacgcggat aaaaccgcgt
      901 gtttacgttc agtagatttt gcgctttgcg caaaactcgc accttcggtg nnnnnnnnnn
      961 cggccatcgc attcgccata atttccaagc tgtcggcaag tgagcgcata ccgtttacaa
     1021 cgtcaagtaa aagtttaatc ctgctcatcc tataccagcc ccctcattta cctcatgtat
     1081 ggaaagctcc tccacactgt caccggggac aagaattgtc attttacacg gcataccgaa
     1141 taagagacga aggaacttct ctcttaccga aacgctgcgg aagttgacaa tactgtctgc
     1201 tctcggtttc tttgaaacac agatttttaa agtatgtttc atattttatc gtcctttccg
     1261 aaaggctgat ttttaatgcc cttcactata cggacagata aaaggtcaaa tgtgaggatt
     1321 tttatataaa cttttttaat tttttatata ttgtcctaag acgatgagaa atagccgaat
     1381 gatctacacc ttccttcttt gcgtattcat tgactgttac tccctcaaag tatatggctc
     1441 tgataagagc ctgctgtttg cttgaaagat gtgtgagagc ctcgtttaat ctctccgcat
     1501 cacccaatcc ttcaaaaagc gctgatattc ccttgtcctc tactgcataa taagcaccct
     1561 catatgtacg cgcctcaagc gaataatgtc tgcgtttttc ctttttgtca ttattccgct
     1621 ccagcctgtc aagctctgca agaacactcg cccactcatc ggaaacttca agttccacat
     1681 ctccgtctac aaactcatat ttgattttca taaaaagtgc cgcctttctg caagaaaaac
     1741 ggcactctaa caagccgaaa aaactcgcat ttttttaatg caagccattc ggctgttcta
     1801 tgttttttga caaaatcccc ctttccatcc aaagggcaaa aaaacgcccg gtaaacagcg
     1861 ttaaaaacta ctgtttaccg agcatttccc cattaaattt attttgcttt tatgttaaag
     1921 ctattttaca agctccaaaa gccacggtgt aaccggcttt gctattgcgc gggcgtttaa
     1981 gtatgccatt tcaagcgtca gacaggtatt gcctaaataa tacccatcca tcaccgtcag
     2041 cgtcatcgca aggtcgggct tgtccatttt tgtaagatat atcggaagaa ggtattgcac
     2101 ccgtcccata tacccctgtg ccgaaacaag tcccggctcc accacaactt ttcgtcttgc
     2161 aagctctacc gctgtttcca gcagcagagg tagtttttag ccttgcgtat cttggcggga
     2221 agtctgtcca agttctcttc atcgcccaaa atatgctcaa catttactct tattggccat
     2281 tcgggattga agttcagacc ttgctgcgcc atcggatata tcggccgttc cggtaaaggc
     2341 tctatgtact tgagccacgg cgacaattca tcgcagaaac ctctgaaata ccattcaagc
     2401 atagagtcca tctttttgtt tctgccgaaa aaaccgtata ttcctttata tcttggcgta
     2461 taaagtcctg tatgaaagca agcacattca ttttctacat gaaaatactt tgatgctttc
     2521 tcctctaccg attccgaatt aaattcaata gcctgtttgc gaaagatagt atgtatatat
     2581 cgttctaata tcggtgtttc aaaattcttt gtttcaaccg caggtttctt aaactgccat
     2641 gcctccggca aagccataca ttttaaatca taaagatgcc tgtaccattc cggtacatac
     2701 gcaaaatcga ataaatcagt ttgtaacgtc atcattgcat caccctgcac tttggtagtg
     2761 cctcaagata ccctgcctgt atcgctgctt tcaaatcctc atcaatatat cgtctcatat
     2821 ttccatcacc atcaacttcc tctactgtgg acaacgcatt tatataacta tcgtaatacg
     2881 ctaaaacttt catttgcgca tcatggtctc cgtgaactgc gcgatacgct gtttcataat
     2941 caagtttttt ctcgtccatt atgtatgttt cctttcataa taatctcgta taaagttaaa
     3001 agcttttctt ttccattggc tgattgtcgt ttcagagaca ctaaacaatt ctgccacttc
     3061 ttttcttgat aacccatacc aaaattcaag tatcagcact tctttctgac actccttaag
     3121 cttagtcata gcttgatata gccactcggt tgtaacaaca caactatgtc cgtgttcatc
     3181 tgagacaaca aattctgatg gatacatatc ctcttttccg tatgtctcca tcacatattg
     3241 catcacctcc gcaccgccgg tttcattatt tttttgtttc tttattacag cagtcttttt
     3301 tctactcata ttannnnnnn nnnctatcaa aaatcgccat atctacatta ttatcggata
     3361 aggaagttga cacaaaggca cccccttctc cgtatacagt tttgtgaatg agtcttttac
     3421 ctcctctgga agactaagac tccgaatgcg cggatatgtt aagcaaatta ctacttttta
     3481 ttgtttttgg gtacaaaaaa taaagcgcat tacaaaacgg tttttaacca cttcataatg
     3541 cgcactatta ccatcatttt attgttttat gaattcatat taaacataat ttttccctac
     3601 ttacatcatc acaaatcact atttcctttg catcaacgaa atctatactt ttatcatatt
     3661 atttactctt tcgcactcaa tgctgcgccg ataatccctg catcataaaa ctgccatgtt
     3721 cataacacat acctttttat cttgctttcc actcatagat gctatatata atac
//
LOCUS       DAWACS010000106         3755 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2659_length_3755_cov_2.143243, whole genome shotgun sequence.
ACCESSION   DAWACS010000106 DAWACS010000000
VERSION     DAWACS010000106.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2659_length_3755_cov_2.143243"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3297..3306
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1214 a          648 c          872 g         1011 t
ORIGIN      
        1 taccgccgcc attttgccga caggctcatc agcaacaaga gtgctgaacg taagacagga
       61 cattaaaatt acaatcgaac agaaaacaga taacaacctg aatattctgt ttttcaacat
      121 cacacccccc tatgtttaca taattcttaa tattatacat tatacaagga aatctgtgac
      181 aattcaactt gatttttgtt aagagcatat tacaataatg tggcatttta ccctttacaa
      241 atttcaaaat gtgtattata attaattaga tagtagcaaa aaatagggta gtgccttatt
      301 ttttatttgg aaggaaggat acgaaatgat aaaagcggca gttctcggag caacgggata
      361 tgcaggtatt gagcttgtaa gattattaac atcacatccg gaggtaagta ttgaaatact
      421 cggttcgcac agttttgcgg gacagaagat aagtgatgtt tataagaact tcaatcatgt
      481 acttgaaaag gaatgtgaag aacttgacct tgataaggtc ggtgcttgtg atgtggcatt
      541 taccgcactt ccgcacggtg cgtcaaaatc ggtaattccg tcattacttg aaaaaggtct
      601 taaagttatc gaccttagcg gtgattacag atatgatgat gcggcagtgt atgaaaagtg
      661 gtacggtgaa aaacattcat caccggaact tttaaaggag tctgtttacg gacttcccga
      721 acttcacaga gataagatta agaatgcaag acttatcggt aaccccggct gttacacaac
      781 ttgttcaata ctcggaacag ttccgctttt ggcaaataaa attgctgaaa caaagaatat
      841 tattgttgac gcaaaatccg gtgttacagg tgcaggacgc ggtcttaaac ttcaaaacag
      901 tttctgcgaa tgtactgaaa atatgaaagc gtataaggta gcaactcacc gtcatacttc
      961 tgaaattgag caggagctta gcaatgttgc cggtgaagaa ataatgattt cgtttacgcc
     1021 acaccttatt ccgcaaaaac gcggaatact ttcgacaatc tatgtaaatc taaacagaaa
     1081 gtcaagtgcg gaagaactaa cggaaatgta caaagaattt tataaagacg aattttttgt
     1141 aagagttaag gaagtgggcg acttgccaga aacaaaacac gttgcaggtt caaacttcgt
     1201 tgatataggc gtgtgctatg atgaaagact tcaaagggca gtagtcgtat cgtcacttga
     1261 caatattgta aagggtgctg cgggacaagc cgttcagaat atgaacttat tattcggctt
     1321 ggacgaaaag acagccctta caaatccggg attttattta taatagaggg tggaagtaat
     1381 gcaagaactt ataaaaaaag cgggaatact tatagaggca cttccgtata tacagaaatt
     1441 ttcgggcaaa accgttgtta tcaaatacgg cggtaatgct atgataaacg aagaattaaa
     1501 gaactccgta atggaggata ttactctttt gaagttcatc ggacttaatc cgattgtcgt
     1561 acacggcgga ggtcccgata tttcaaaggc acttaacgaa cgcggtgtaa agagtaaatt
     1621 cgtaaacggt ttgcgtgtga cggacgatat tacaatgcag actgctcaac aggtacttat
     1681 aggtaaaaca aataaagaaa ttgtcgcact tcttaataca aacggcggtc gtgcagtcgg
     1741 tatcagcggt attgacggca gttttatcga gtgtaaaaag cagtatacgg aagtggacgg
     1801 cgaaaaggtt gatataggct atgtcggcga tattgttcac gttaagcatt gtttgattga
     1861 cttattgtca tcagatcaat atatccctgt tatcgctcct atcggtacag atgaggacgg
     1921 taacagctac aatattaatg cagatacggt tgcggcggca gttgcggcgt ctgttgaggc
     1981 ggaaaaactt attttgctta ctgatgttga gggtattaag gataaagacg gtaatgttat
     2041 tgccgaaata cataaaaatg aaatatacga aatgatacat gacggcagca tcagcggcgg
     2101 tatgatacca aaggtacttg gctgtcttga atcaatcgac ggcggtgtta cgggtgcgca
     2161 tatcattgac ggacgtatac cgcattgcct gttacttgaa atctttacaa agacaggtat
     2221 tggcacgctt attaagagtg ataaatatta ataaagagag gaatttatat aatgagaatg
     2281 tctaacttgc ttatgccgac gcttagagaa gtaccggcag aggcggaaat aacaagtcat
     2341 aaattaatgt tgagagcagg tcttatcaga aaacttgcgg cgggtatata ctcatatctt
     2401 ccgcttggac ttcgcacttt gcaaaaggtt gaacagattg tccgtgaaga aatggacaac
     2461 gctggtgcac aggaactttt gatgtctgca cttttgcctg ctgaggctta tcaggcgtcg
     2521 ggcagatggg aagtgttcgg acctagtatg ttcaaaatga aagacagaaa cgaccgtgat
     2581 ttctgtcttg gtcctacaca cgaggaactt ttcactcaaa ctgtaattga cagtgttcgt
     2641 tcatataaag aatacccaat gactttatat caaattcagc ataagtatcg tgacgaggga
     2701 cgtccgagat tcggcattat aagaagccgt gaatttataa tgaaggacgc gtacagcttt
     2761 gaccttacgg aagaagggct tgataagtca tatcaaaaaa tgtatgacgc atattgcaga
     2821 atattcaacc gtcttggact tgatttcact atcgttgacg ctgacagcgg tgctatgggc
     2881 ggcagcggaa gtcaggaatt tatggttaag tcgccaatag gcgaggacgg tattgcgtat
     2941 tgtgacgatt gcggatatgc ggctaactat gaaaaagctg aatgtattcc tcaagaaaag
     3001 ccgtcacctg agggtgattt tgatatggaa aaaatccata ctccgaatgc tcatacaatc
     3061 gaagaacttg taagtttctt aaatgcagag gcttataact ttgcaaagac tataatatat
     3121 aaggctgacg ataagtatgt tgccgcaatg gtaagaggtg acagagaagt aaacgaagtt
     3181 aagcttaaaa atcttgtcgg ctgtgtggac gatttggaac ttgccgagcc gtttatggta
     3241 agacagatta caaatgcaga agttggtttt gcaggtcctg tgggacttga tattccnnnn
     3301 nnnnnncgta caatcgaaac aggcgacgtt tgccctaagt gcggtaagcc tataaagact
     3361 gctcaaggta tcgaagtagg acatatattc aagcttggta caaagtattc tgacgcactt
     3421 ggactaaaga gccttgacga aacaggaaag agcaagactg ttattatggg ttgctacggt
     3481 ataggcgtta cacgttgtct tgcagcggct atcgagcaaa acaatgacga aaacggtatt
     3541 atctggcccg tatcaatcgc accgtatcac gcaatcgtta tccctgtaaa ttctaagaat
     3601 gaagaacaga gtgaaattgc cgaaaaagta tataatgacc taaaggcaaa gggaatagaa
     3661 gtattgcttg acgacagaaa tgaaagagcc ggcgttaagt tcaaagacgc tgaccttatc
     3721 ggtattcctg taagaattgt cgtaggtaaa aaatg
//
LOCUS       DAWACS010000107         3742 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2671_length_3742_cov_2.822891, whole genome shotgun sequence.
ACCESSION   DAWACS010000107 DAWACS010000000
VERSION     DAWACS010000107.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2671_length_3742_cov_2.822891"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    217..226
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          953 a          958 c          576 g         1245 t
ORIGIN      
        1 agtgtgtccg ttgcctcgat ttttgcatta tacaaattca aaaccgcata catagtatcg
       61 tcactgtatt cctgccaacc tacaaaaccg ccgtcataca ttttctgaat acgttcgata
      121 ctgcgttcgt aaaactgaat atgactgtcg cctgtttcgt cccaatcatc atatgtatcg
      181 cggatatttt tccaattctt cgcgtcactt ttccacnnnn nnnnnnttct caagcttgcc
      241 gattttcaaa tcgacatcgg ctttgtactc atagaaatcc tcccaaattt ctttaatttc
      301 ctctgccgaa tatgcggttg acgtaaccat ttccgacacc atagcattat agttgtaaag
      361 ctgtcttttg tacgattcaa tctgctcgtc aacgcccata tcattcactt gcacctgata
      421 tttaatccaa gcgtcggaat gttttttgta gtctgaaagt gcggtttcac tcgccttttt
      481 ttcttctttc cagattttct tctgcgactc gaaaatctcc tcttgaattt ctttcacaac
      541 ctcgctgtca taactgaatt gctccatcaa ttcattccaa agccttaatt cctctgtcgg
      601 agatacatcg tttgtttttt tgtaatggtt taaatcagcc tttttaattt caaacatttc
      661 attgtttttg ccggacgcat aatgcggaat acccataccg ttcattatcg cctttgtttc
      721 gtcagccgta taaaccttgt cgccttgcga cagcggaaca accacatcac gcccgctaaa
      781 catcataagt cgcccgttat gctcgataag ctcacgcggg tcactgacgc ccctttcatc
      841 atttacaacc gccaaaccgc tttttgcata ttgtgttccg tctgccagtt tcggcattcc
      901 ctgcacattg tacttaacag taacattgac tgttctgtcc ttcaaactgt ttatacttgc
      961 tcttgccgaa ctcacaccgc ttgcggtatt gtcaactgcg gtaatattaa tttttgcgtt
     1021 tttgccgtca aattcactca ttattttacc tgccgatgaa ttaagtgtag caagtgcatt
     1081 ttgtgccgtt gccaaagcat ttataagtcc agacgcgtca cccgttatac tgaccgataa
     1141 attagccgcc tctgccataa ctatacctcc tctccgtaaa acattctcaa atgttcgtta
     1201 tcgtaatcct ctgtatcttc attcaatccg tcaagcattt taaacagtac ccacggattt
     1261 tgtctgccaa cttcgtcagg cagtatgtgg tggtacgtta ggaacatcgt atagatgtcc
     1321 ctaagcaccc cgctgccgcc tgacgttact cgttttttga agaaagagcg tcaacgtaaa
     1381 acccccaaag tgcaagacac atctttgcac gttcgttcac gtcaacggaa tcgataatat
     1441 cctgagttgc gtccgtaccc tcaaacatat agtcaagtgc atctctgcaa atgctgagcg
     1501 gacctttctt attttcatca ttatgtgcgt cattaataat gcacatcgcc tcaaagtcaa
     1561 acggctttga aacgtattta accttatctt ttacaaatgt taatgtatgt tgcataaatt
     1621 tctccttata ccgtctgcgg atagtaattc atatccgtaa accacttgtt ttcaaattca
     1681 tctcttgtga tatttgacgg caaatcgctt tcatcaaaat acgcataata attgttgtca
     1741 aaatcacgtt gtaccgccgt gtatgttgcc ttagcggttt gcttgtcgat acttccgctt
     1801 gacggctttg tctttccccc aacattcgat gcaaaactgt atgaaccctt gtagtatctt
     1861 acataacggt acgagccgtc ggacttcaaa attctccatg ccacaccgaa atatactgtt
     1921 ttaacatcgt ttccgacttc aaccacaccg tttttgcgtg tcaatccacg ccacattgcg
     1981 tcaacctccg gcggaatatc ggcatttgtt atatcgtgtc ccaatttttc gatatagctt
     2041 gacgcctcat acgcaccgtt gtccgcatca aaaacatcac tgccgccgct gtcggtcggt
     2101 gcaatttcaa ccgttccttt caaattatac gcttctccgt attccgttcc gctaacgctg
     2161 tcctcattca ctttaaaaaa agtatacttg tccacaccta ttgtaggtaa aggtttcttg
     2221 ttcataattt ttcctccatt ttttcatatt ttgtcttaaa gagatatatt tttataacgt
     2281 aaaatacttc tgaaatctca ttgtcttatg atatattttt ttatcatttt cgggaacgtc
     2341 catagacatc tccctcaccc aaccgtcctt tgtcattact tcattgacct ctgccgccaa
     2401 atcactgcat tctttcggaa tttccgccca tatgtcaatt tgaatatgcc cgtcttgaat
     2461 actctcgtca ttgtcggcat aaaatccgcc gcactccgtc accgtataat aacttataat
     2521 cggcagatta ttaaaatttt tcggatattg aaatactgcc ttgcatttaa catcactgag
     2581 tgacttttca gcctccgcat taatatcaat catataatca cccctttatc gcctctgcga
     2641 ttgcagacaa tattgcttgc gtgtttgata aaagtgccgg cacaagatac ggctgtgccg
     2701 ccattttcga cgtaccgaat tcaacaaata ccgcatattc cgtatcagcc gatataaccg
     2761 ccgtatttcc gccggactgt acattaattg acgactgcag ccgtcccgta tcgacagggc
     2821 aaagactttt tgcactttca cataccgttt gagcaccctg accgattata tccgcaaaat
     2881 tattttctat attatttgca atatccgaaa gtccattatc ataattaata tcaatctgca
     2941 tttttctcac tccgcatcat cgccgtcata ccgaaattcc aacattcagc ctttataatc
     3001 tcaaaaaatt cgtcaccgaa tttagcatat ctgcctattt ttataaggtc tgttttgtca
     3061 cagaacactt tatatttttt attttcggtc aaaccgtata tacggttttc gagttcattt
     3121 tcatatggct gaatatcaca cacaacactc cccaaaagtg ttttttcacc ctttttttca
     3181 tatgtatttt caccgacttc gtacacctcg atttcggtgt cataaaatcg ttcaaacgga
     3241 tttatcatcg gcaaccacct ccgacggcac tttaactttc ctgctgacaa acggctttag
     3301 acgtattgca tattctttca aataatcata gttgtcgtca gagtattcga ccttcctctc
     3361 accctccgta accgacttaa caccgccgtt ttgatttgcc gcatatctct tgaccgtaag
     3421 cgtcggaata aagcttacaa tctgtcttgg cagaacatca atacgacagt atgccagcac
     3481 agaattaaca gtatcctcaa tcaaaagcat aagaacatcg tccttgtcat tgtctttaat
     3541 tccgagcagt aatttcaccg cgtcaagaat ttcattttta gtgtccccca ttttcaacac
     3601 agcctttctg acctattctt attcggcagt cttaaacaca taaatcgcac tctttttatt
     3661 ttcaagcaca aatgcgtcat aatacacact gccttcaacc agccagccgt tgattcccgg
     3721 cggattatcg tggattttgt at
//
LOCUS       DAWACS010000108         3737 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2675_length_3737_cov_2.148289, whole genome shotgun sequence.
ACCESSION   DAWACS010000108 DAWACS010000000
VERSION     DAWACS010000108.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2675_length_3737_cov_2.148289"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1552..1561
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1997..2006
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3208..3310
                     /estimated_length=103
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          974 a          690 c          751 g         1199 t
ORIGIN      
        1 ttgcacgcgt tacagcggtg taaaataagt tgcggctcat aagcataggc gagaatgagc
       61 atacgggcat aatcacgata gggaactcac tgccttgact tttgtggacg gttatcgcgt
      121 acgcaaggtc aagctcatca agattggtaa acggatattc aacgagctta tcctcatcaa
      181 acacgatatt catacacttg tcgcggacgg aaatgctctc tataattccc atatcaccgt
      241 tgaaaatccc cataccgtcc tcgccgtttt cacgtgaata gaccatatcg taattgtttt
      301 ttgtctgcat aactttgtcg ccgacacgga aagttatatt gccgaaaacg tgttgcggac
      361 gtctttcgtc atatggattg atgtgtgact gcaacagttt gtttaatgca atcgttccgg
      421 caagtccttt ttttgtcggt gataggactt gtattgatga aatcgggttt acattatagc
      481 ttttcggcag tctgtttttg aataaatcca tcaccgtaaa agcactgtcc tgcggtgttt
      541 ttcttcttaa aaagaagaaa tcgcttgatt ttaccgataa atcgggaagt tctccgtgat
      601 tgattttgtg tgcgtttacg attataagac tttcttcggc ttgcctgaaa attttgttaa
      661 gctgaattac cggtattgtt ttactttcga ttatatcgtg aatgatgttt cccggcccga
      721 ctgacggcag ttggtccgca tcacccgaaa ggataattct tgcacctgtt ttgaccgctt
      781 ttaaaaatga cgacataaga tttacgtcaa tcatactgac ttcgtcaaga attataacgt
      841 catacggcag tggattttct tcgttgtgcg taaaaatact gtaaccctca gaagatcgtt
      901 gagtgcataa aagacggtgt attgtctttg cctcgtaacc cgtaacttgg ctcattcgct
      961 ttgcggcgcg tcctgtcggc gcggcaagtg cgattgaaag ctttaaatct tccaaaagct
     1021 ttattattgt gtttattgtt gttgttttac ctgttcccgg accgccggta agaatcatac
     1081 aacctgccga aagtgcggtg acaacggcat tgcgctgctc ggcggcaagg tataaatttg
     1141 tttttgcttc aagcgcgtca atggcttttt ctgctttttc ttcggtcatt gtgaattttt
     1201 gcgtgttttg cgacattgca ataagtcggc gcgcaatgta atattcagac tcataatagc
     1261 tgtaaagata gtatgcgtca attccgtcaa ctttatcttg gaacagttct tttgaagaaa
     1321 gcaattcgga aacagccgca attacttcgt catcggtgac ttttaatgtg tagacggtat
     1381 gctcgattaa tacatctttc ggcatatata catgaccgct tgtatatgcg gcactgcgta
     1441 aaatatgtat aattcccgaa cgtatacgca taatgctgtt tttaggcaat cccatattaa
     1501 aggctattgt gtccgatgtt ttaaaagaaa tgccgtcaac catatcagaa annnnnnnnn
     1561 ngaatatatg gatttttctt tatcagttca accgaatttg caccgagaga attgtgtact
     1621 tttactgcgg ttgttgcgga aattccgtac tgttgaagga acataacgat attttgcata
     1681 gactgtattt ctgcaaattg ttcgcctatt ttcttcgctt tttctttgct tattcctttg
     1741 atttcggcaa gttttgacgg ctgggtaagc attatttgaa gtacgtccag tccgaaatgg
     1801 tcgacaagtt tttttgcggt agcctcgcgt ataccgctta ttattcccga acttaaatat
     1861 ttaataatag actcctcgtc cgacggcata actgtttcat aatattccgc tttgaattgt
     1921 tcgccgtaat cggggtgagt tgtccaatta cccgacaggg caacgctttc accctctgtt
     1981 ataaacggca tatatcnnnn nnnnnnccgt tgtcgggatt ggaatatata atttcttcaa
     2041 ctgtgcctgt tactgtaata atttcactca ttattttgct ccgatacggc tattgtgccg
     2101 tttgaaattc ctgtgatttc tgcgcctttt gtttcaaggt attctatgta atcaatctgt
     2161 tcttttctga tagtttcgcc tgtgtatata accggtactc cgggcggata aggtacaacc
     2221 gtactgcaag aaatatgtcc gattgaattc tgtaatttag tgtccctttg atttgagaaa
     2281 aatgcttttt gcggaaacag tttttctttg caaatcggtg gaggcgttag atttaatgtg
     2341 ctttttgctt taggtgtatt gtttgtaatt ttgataagct catcaaaaag cacgtccata
     2401 tccgattgcg tgtttgacgg cgtaacgatt aaaactatgt tgaataaatc agccatttca
     2461 atgtctatgc cattatttga aagtatgtca tcaacgtcaa agcctgtaat atccaaattt
     2521 gaaaaattca gcacaagcct tgtcatatcg tcattttcaa gaactttgat acctgtatcg
     2581 acgatttttt ctttaaatga tttgcacata tcatatgttt tctcccacat tgcaccgtcg
     2641 gttaattcat ctcttgcaat gtccgccgat gacgcgatga cgtatgacgg acttgacgat
     2701 tgaaacatat aaagtgattt ttcaagacgt tttctgtcga ttaaatcgcc gtttatgtga
     2761 agatacgaac ttccgttcat tgcattaagc gttttgtgag cactttggca tactgcgtct
     2821 gcatatttta ctgcggtttg cggaaataaa ttgcttgcga taaagtgtgc tccgtgcgcc
     2881 tcgtcaacaa gtaaaggaat attttttgcg tgacattctt cggctgtgtt tttaatgtcg
     2941 gaacatattc cgtagtaatt cggtgatgta attataaccg cgtcaatgtc gtccgttaaa
     3001 tgttttttta gtgtccctgt acctttaggg acactaaaac tgttgtctat ttctttcgga
     3061 aacaatttta aattgatacc gagtaaagcg caagtgttta taacgctcat atggcagtcg
     3121 cctgccgaaa gcagtgtgcc gccgggcttt acgcctgcac atatcataga ctgaattgca
     3181 gatgttgaac cgcctgtgag tatgtagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn atacagtggt gctgacatag acacatctcc ttagttgttt cctattatta
     3361 tataaaattt ttgcgtatat gtaaagtaat atggaataca aaattgaaaa acacaaaaga
     3421 atatgatata atagtgacag gttcaataac aatatttatt tttggaaagg acagataaat
     3481 tatgaatgta tatgatgcga taatgaaaag aagaacgata agaaagtttg aacaaaaggc
     3541 agtaagtgaa gataatcttg tgaaacttgt ggactgtgca agagttgcgg catacggcgc
     3601 gaatgttcag ccgcttaaat ttatgattgt aaacaacagc gaaatacttc aaaaaatata
     3661 cccaatgaca aagtgggcag gctatcttgc agacggtgca ccgaaagaga atgaacgccc
     3721 tgcggcatat atagcgg
//
LOCUS       DAWACS010000109         3714 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2692_length_3714_cov_2.532113, whole genome shotgun sequence.
ACCESSION   DAWACS010000109 DAWACS010000000
VERSION     DAWACS010000109.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2692_length_3714_cov_2.532113"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1631..1640
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2016..2025
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3433..3442
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1128 a          604 c          881 g         1071 t
ORIGIN      
        1 tgcctgagag ttgtagttcc gatgaagctt attgaaggtg tttcggatag cgtcctgaaa
       61 gaaaacgtat atgtttaaag acagccattg tttgatatag cataaatgat atatcaaaca
      121 atggctgctt tttgtatatt tcttcgaaaa taatggatat ttttttataa aatatattga
      181 cattctatat taaatatgct aaaataaact taaatgatat atcaaacaat aaaacagctg
      241 tttgatatgg gaaagagtta aaacatttaa aaagaggagg agtaagaaca tgaatagtaa
      301 aattaaaaag gctttgtgcg ttgctgtggc aggtgcctcg cttgtcggtg ccttgagcgg
      361 ctgcagtaat agtaaagagg tttcaaatgt tgttacatat tgggcaggta ttccgatggg
      421 tgacggaagc agcgatgcag aaagtattcc gtttgttcaa tatcttcatg aggctacagg
      481 tatagatgtg gatttcacga ttgcctcaag cgatggggga cagagaacag agcagttcaa
      541 tatattgatt gcttctaatg agtatcctga cattatggaa tcaaactggg caggatttaa
      601 aggcggcgct cagggagcga ttgaccagaa agtcatagta cctctaaatc agtatatgga
      661 tgagggtaag ctgccgaact ttgtaaaata ccttgaagag catcctgaga ttgacaaact
      721 tttaaaaact gattcgggcg agtattatac aatcccgttc atccgcgagg atgataaatc
      781 gcgtatgttt aacggaccta ttctccgtaa ggattggctg gatgaacttg gacttgaagt
      841 cccggaaact attgacgagt gggaaaatgt tcttgctcag ttcaaggaaa agaaaggtgc
      901 gactgcaccg ctgacaattc tctctaactt ctgggatgtg ggtgcattta tgggcgcata
      961 cggcgttaaa aatggattta tccttgacga tagcggcaag gtagtatatg gacctatgca
     1021 ggacggatat aaagagtttc ttataaaaat gaatgattgg tataagaagg gtcttttgga
     1081 taatggattt gctcagattg acggaaatat gcttaattct caaatgacgt cgggaaaatc
     1141 aggcgcagca gtattctcgg caggcggagg aatgggtaaa tggcttggcg caatgtcaga
     1201 cgatcccaca tatgaccttg taggcgcgaa attccctgtg cttaacaagg gcgacagacc
     1261 gagattcggt acgaaagcat ctgcatttga tggttcaggt gcttcaataa caacatcatg
     1321 ccctgaagag agaattgaca gtgcactgaa gcttcttgat tatgcttaca gcgatgaagg
     1381 aagaaatctg ttcaacttcg gtaaagaggg tgttgactac acaatggttg acggcaagcc
     1441 gacttatact gattatgtaa caaacaatcc tgacggaaaa acaatgtctg aaatgcttgc
     1501 tcttgaaaca agagccacag gcggtccgtt tgctcagagc gtaagatata cggagcagtt
     1561 ccttggtctt cctcagcaga gcgctgccgt tgacaactgg agagatacag atgatgaaaa
     1621 atattcgctt nnnnnnnnnn ccggctgtta tggcacttac acaggaagaa tctgatgaat
     1681 atacaagaat aatgactaat gctaatacgc ttaagggcga aaagtttgtt aagtatattc
     1741 aggggacgga aagtattgat acttgggaca gttatataga tgagcttaaa aatatgggta
     1801 ttgaaagagc tatagaaatt gaacaggctg catacgaaag atttttgagc agataaattt
     1861 ttgcgagggg gcttcggaaa tgcgattaag aaaaaataag gcaaacactt taaaagtaag
     1921 caaccgcgga gttataagag cggattttca cagaaataaa tgggcgtatt ttatgtttat
     1981 acctgtactt gtatggtata ttgtattttg ctacgnnnnn nnnnngttgt aaactttccg
     2041 atacctatca tatttgcact tttggtgaat gaacttagaa gcaaaaagta tggtaagctg
     2101 gttcagactg ttacaagtct gccgcacttt atatcattga tggttatatg tggtatgatt
     2161 aagacattta cgatggatac cggcataata acaaagattg tcggagcgct tacgggagat
     2221 tatagcaacc ttctgaataa accgggagcg tttgttccta tatacatact ttcaggaacg
     2281 tggcaggaaa tgggctggag cgctatcata tacatagctg ctcttgcggg agttgaccag
     2341 gaactttacg aagcttccgc catagatggc gcaggaaagc tacggcaggt gtttgcaata
     2401 actcttccaa gcattattcc gacaatagtc gttatgctca tactgagaat aggcggtctg
     2461 ttaaatgtcg gatatgaaaa gattattctg ctgtataatg agggtatata tgatacggct
     2521 gatgtaatat cgtcatttgt atacagacga ggtattgttc agagcgactg gagctacagt
     2581 gcggcagtcg gcttgttcaa ttcggtaatc aactttatac tgattatcac ggcaaacaag
     2641 ataagcaata gactgaccga aaacggttta tggtaaagga gaggggttgt gtttgaaatg
     2701 aaaataaaac gcagtaaagc agaacaagcg tttaatatat ttaatattat acttatgttt
     2761 gttctaatgg ttgtaactct gtatccgtta ctatatgtat tgtttgcatc gctatcggac
     2821 gcgtcgaaac tgatgcagca tagtggactt ctgttcaaac cgcttgggct caatttgtcc
     2881 tcgtataaaa tagttataca ggataggcag attctcagcg gctatatgaa cacaatcata
     2941 gtagttgtgg caggagtact gataagtatg gtgcttacta tacttggagc gtacactctt
     3001 tcgagaaaga acctttattg gggcaagatg atttcgatga tagtaattat tcctatgttt
     3061 ttcagcggag gattaatacc gttttacttg ctcgtaaaga accttggaat gttggatagc
     3121 ctttgggcgc ttattcttcc aaccgccctg tctacatata acatgatgat aatgcgtacg
     3181 gcattggcgg gggttccaaa ggcgcttgag gaagcggcgg aaattgacgg ggctaatccg
     3241 ttggtcgttc ttttcagaat tcttattccg cttacaattc ctacgattgc tgttttaatt
     3301 ctctattatg cggttgcaaa ctggaattcg tggtttaatg ctatgctgtt tttgccgaaa
     3361 gcaaaagaaa agtggccgct gcagctggta ttaaggaatt tacttattca aaatcaaacg
     3421 gacgatttaa cannnnnnnn nnaaatatgc tacaattata gtatcaacgc tgccaatact
     3481 atgcctgtat ccgttcctgc aaaaatactt cctgaagggt gtcatggtcg gcagtgtgaa
     3541 gggttgatga tttttacctc agaaaaatat aaattgcagt gaattttaat gaccggcata
     3601 gaatgagagg gacgggtttt ccgtcccgct cgttttataa aataaaactt atgcgaatat
     3661 cggaggattg ttatgaaaaa ggaatattgc gttatatcac atacgcattg ggat
//
LOCUS       DAWACS010000110         3686 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2722_length_3686_cov_2.526026, whole genome shotgun sequence.
ACCESSION   DAWACS010000110 DAWACS010000000
VERSION     DAWACS010000110.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2722_length_3686_cov_2.526026"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1186 a          669 c          742 g         1089 t
ORIGIN      
        1 gtattacatt ggtatcgaca tttcaaaatt caagcacgat tgtgccgtca ttgacgactc
       61 cggagaaatt gttactcctt cgtggtcttt tacaaatgac gctgagggat tttcgcaatt
      121 aaagttattg cttaattccc ttgatacaga aacaagaata ggacttgaat ctaccggtca
      181 ttatggtatg aatctaaagc tgtttcttga atccaacaac ttttcattca tggagttcaa
      241 tcctctgctt atcaatcgtt tcgttaaatc caagtcccta cgaaagacta aaagcgattc
      301 tattgattgc atgatgattg ctcactattt aatgacggta gagtacaagc cctatccatc
      361 atcattttac cacacagaga agctaaagtc actaacaaga ttcagagatt ctcttattag
      421 gctcagaagc cgccagcttg tagagctcac aaacatctta gataaggtgt tccctgagtt
      481 taaaccattc tttggcagta ggttttcggt tacagcatta tacatactct ctaattactc
      541 atcgcctgaa aagattgcta acatgaactc taagtcttat gaagcactac gcaaattatc
      601 tcgcggcaga ttttcaacag tagattttgc aaagcttaaa cagcttgcta aaaatactat
      661 aggtactact gaagattatt tactcttaca aatgcagatt gttttggata tttacagaca
      721 attggattca aaggttgatg agacagaagt ttctatcaag gaatgtgttt ccgatatgaa
      781 tccgcctatg ctttctgttc cgggaatcgg tattgaatct gctgctgtaa tcttagctga
      841 atttggcgat ttctctaaat tcaagaatgc ttctcaaatg ctttcctttg ccggattaga
      901 gccgggatac ttccaatcag gaacatccga gacaaccggt aaaatggtta agcacggttc
      961 atcatatttg cgttatgcac ttatgaactg tgcccagaca gttgtaaatt atgaaccgac
     1021 atttgctatg ttttactcta agaaacgtgc agagggtaaa cctcatcgtg tggctttatc
     1081 gcatgttgtt aaaaaactac tgcgtgttat ctatacgctg caaactaaaa acattgcata
     1141 tgatgctgat tttgtaaggt agttactaac taacatacta ttttttaagt ccttgcaata
     1201 caggactttt ttgccatgtt gtttttcagc ttttaaaaat ttttgaaaaa cttttcaaaa
     1261 acctcttgac ttctaatagt tagtcatctc cgaaaaattt atcaagtgcg tgtgtttgct
     1321 ttaatgctgt aacaaggaaa attgctataa cagtaccgcc tgccgaactt attataaacg
     1381 gaagtacata tgcgaaaagt gcagcctctt tacccataac gtaaacggcg ataggataag
     1441 cggcaagtgc accgattata cctgttccga acaattcacc gatataggca agcggcaatt
     1501 tctttgaaaa tttatagcac aatccaccga taagtgcacc gcacatactt cccggaaatg
     1561 caagaagtgt acccataccc gttgcaagac gaagaagtga tgtgataaat gccataatca
     1621 ctccgtaaaa cggtcctaaa aatactgacg caattatgtt tataagatgt tgcaccggaa
     1681 aacattttga cgcgccgaca ggaaagttta acggcgagca tacaacgcct acagctataa
     1741 gtacgcctgc tattgctaat tttcttgttg taattttatt catttgtaaa aatctccttt
     1801 gattgaattc ttctaatatt atagcatacg caatattttt agtcaataaa ccgtggacga
     1861 atgaaatatt atgaaaaaat attataaaaa ctcttgtgca aacgtgaaaa atttgttata
     1921 atgtgttata catagattga cgagaggaga atagaatagt ggaaaagaag ggtagatacg
     1981 gagaattcgg cggacagtat gccgctgaaa ctcttatgaa tgcacttatt aatttggagg
     2041 aggcatttaa caagtacaaa gatgacgagg attttaaccg tgagcttaat tattatcaca
     2101 agcaatatac gggcagaccg tcaattttgt attatgccga aaaaatgaca aaggatcttg
     2161 gcggtgcgaa gatttatttg aaacgtgaag atttaaacca tacaggtgca cataaaatta
     2221 ataacgtaat aggtcaatgc ttgctcgcaa agagaatggg caagaaaaaa gtcattgccg
     2281 aaacaggtgc gggacagcac ggtgttgcaa ctgcgacatt tgcggctgtt ttgggacttg
     2341 aatgtgatgt atatatgggt gaggaagata caaaaagaca ggcacttaat gtatttcgta
     2401 tgaatttgct tggtgctacg gttcatccta ttacatcggg aacaggaaca ctaaaagatg
     2461 ctacaaatga agctatgcgt gcttggacag caaatgccga agatacattt tatgtattag
     2521 gttcagcagt aggtccacac ccttatccgc ttattgtaaa gcattttcag agtgtaataa
     2581 gcaaggaaac aagaaaacaa cttcttgaaa cggaaggtaa actgcctgac gcagttatgg
     2641 cttgtgtggg cggcggctca aacgcaatcg gtatgttcgc cgattttatt gacgagcctt
     2701 ctgtaaagct ttacggtgca gaggcagcgg gcgacggcgt tgatacagaa cgtcatgcgg
     2761 cgtctataac taaggggaca gtcggtgtac ttcatggtat gaagtcgctg ttcttacaag
     2821 acaacggcgg taatattatg cctgtatatt caatttcagc aggacttgat tatcctggaa
     2881 taggtccgga acatgcctac atggcgaaaa cgggcagagc ggaatattat ccgataacag
     2941 ataaagaggc agtggattct tttgtgtatt tgtcaagaat ggaaggaatt attcctgcaa
     3001 tcgaatcatc acacgcaatc gctcttgcaa gaaaaattgc acctacaatg aaaaaagatg
     3061 aaatacttgt cgtgtgtctt tcaggacgag gggataaaga tgtatattca gttgctaagt
     3121 atctcggaaa agatatttca gaagaataat actaaaaacg aggcttacaa ttttgagtag
     3181 cctcgttttg tgcttaatgg aaaatatcta ttatattatc cataacagaa ccgatattcc
     3241 taaaaatacc ctcagtcttt tttgaaattt tgcgacgctc tctgtcggat agaggatcgg
     3301 tcattacacc tactgccgca ccgacagcaa aacctaccag agtacctttt ataaaacagg
     3361 acattttgtg catatgtttt aacctcctta tttaattggt atggtattat tatttgacga
     3421 aacaaacata ataaaacaga aactaaatgg attttcaata taaattttat atttttaaca
     3481 gacggaacag ttttcgccgc ggggcgaatt aatgaaagaa aggattggtg atacttttat
     3541 ggctaatata aaagcgttca gaggatacag atataatacg gataaaattg acaatctcgg
     3601 ctcagtcatt tcgccgcctt attataatat gccaaaagaa gataaggatg cgctttatga
     3661 caagagcgaa tataattcgg tgcgtc
//
LOCUS       DAWACS010000111         3656 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2742_length_3656_cov_2.571508, whole genome shotgun sequence.
ACCESSION   DAWACS010000111 DAWACS010000000
VERSION     DAWACS010000111.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2742_length_3656_cov_2.571508"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3096..3105
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1010 a          802 c          636 g         1198 t
ORIGIN      
        1 ggcggtatac gctccccaac cgccgttaca gattgaacaa gggcaataat gcgtaatctt
       61 aaacttaccc aaatatgtac cgagttgctg atcattgtca acaggcacat cctcaatatc
      121 tatactcgga attgagttgt cggcaagatt atctgcgctg tatttaccgt ttccgtggtt
      181 cggaattatt gcaagccctt cggggatatt aacttccgca caaagctcat cttgatacca
      241 ttcaatatca agtccgaaca tttccataga atcacgtatc ggtatcataa gtgtaccgtc
      301 ctctatgaaa acagcctgcg aaaccatttc ataatcctta ccaaacggca cattatcgcc
      361 gtcagaatcg gtaaagttat acctaataac agcgttgctg tcattaagtc ttaaagctgc
      421 cgttatactt gtcggcgtca tatccaaccc caagccgtca attttattaa tcgcctctga
      481 cgcaaaacgt tctatcggtc tgtccgaatt tgcgtctgct ttaagcgtca aaagtgcggt
      541 ctgagtcggt tggtcccatt caacctccat accggcagcc tccgccattg cccttgcggg
      601 cgtcaatgtt cggttatcga tttgtatagg tttcgtatcc gtaaaattta tgtaactgac
      661 gtcattcgac acaatcccat tcattcttac acttatatca ccgtcagcga acactgcgtt
      721 aatatttcct accaccaata ccgctgataa aactgccaaa acagctttct ttaatttttt
      781 catttttaat ttcctcctac tctctttcta caattttaca atcgtatagg atttgttgag
      841 ttacattaat tgttgagcct gtctacaaaa ccatagaggg tttgcagaca atctgattaa
      901 tcattataat tttcgtatac aactatatcc gttgccgttc cgtcaaccac tcttacaagt
      961 gcgtaattat agctgtaatc gtttgtgaaa tctattgtat tgtccttatc gaaagtgctt
     1021 ttcggaattg ttgaagttat atcgccaact gacgcaattt caacttcatt cttattcgat
     1081 acatcgcaag tataaactat tgtatctttc tttgtatcaa cttcgataac attgtctgta
     1141 ctgccgctct tgtttatcaa agtaagtgaa tttgaaccca ccttaccgat aagtccgaat
     1201 gcgtactgat atctgtcgct tgacgctttt tcaccgtact tcataagctt ccattgattt
     1261 gcaacacttg agccgttttc agtaattaac ttttcaaagt cagtgccgaa atcctgactg
     1321 cccgtaatta tatcttcgtc ctgcggtctg taaataaagt acatatgatt tattcttgtt
     1381 ccggcaacgt ttgatgtaag gctgataacg tcaccctcct caagatttcc tgcgtctttg
     1441 cctttcataa acgaataagc ctccgaagaa gtttcaaatg taacatcatt ttcaatcaac
     1501 gtctttattt ccttgccctg tgcaagcaaa gtaacggcat acatatcttc accgtctttt
     1561 gtagttgttt caacttcctt aacaaccgcc ggtgcataaa ttgcgtctgt tactttttcg
     1621 taaattgacg gtgtgtccgt tgacggctta gttgtcggta ccgttgttgc agacggcttt
     1681 tctgtcgcag acggtgtggc tgtcggtgtt acagacggtg tggctgtcgg ctgaacatca
     1741 tcacttacac tgacttcttt tatctctttt gaatttacaa agtaaacctt tgttttcatc
     1801 cccttatact ctttaggtat atcggcactg aaattgccgt tctcagactt atacatattg
     1861 ctgtagcaaa gcttgccgtt ttcgtcatag cacgaaacaa cggcaatatt ttcgtctgtc
     1921 tgttcaaatg acaatacatt atccgtataa tttgcattaa tgttttcggc aaaagcaact
     1981 gttgacatca ttgccgaaac tgcaagtgta atagctataa ttttcttttt cattataaac
     2041 ttcctctctg aatttatttt gcaatataag acgttacaaa ctgctttgca catcttgcac
     2101 tgcggcctcc gtagttcatc tgccatacta ccgcctcttt ttcaatatcc gcattcattt
     2161 ggatattgtg ctttttaagc atttcggaaa cgatattaag atattctttt tggttaggtg
     2221 cagagaatac aagactgata ccgaaacgtt ccgaaagtga aagagtttcc tgcatttggt
     2281 cgttcacgtg gatttctccg ccctctctgt ctgaccaatt ctctcttatt aaatgtcttc
     2341 tgtttgacgt tgcatatata agcacgtttg taggattagc ctgaagttgt ccctccattg
     2401 caacctttaa cgctctgtac tctgtttcgt gtgattcaaa agtaaggtcg tcaaggaata
     2461 tgatgtactt attcgggctt tcagccaaca cctttgaaag tgacggtata tcctttatac
     2521 aatgcttagg catttcgata atacgaagtc cgtcatcaca gaacatattt aaaagtgcct
     2581 ttacagatga tgatttacca gtacctctgt caccgaacaa caatacgtta ttcgccgctt
     2641 taccgctgac aaacgctttt gtattatcaa tcaacacttg tttttgatac tcaataccga
     2701 caagcgaatc aaaagtaata tcatcggtat tcgatactcc gataagttcg ccgtcatatc
     2761 taaacgcatt atacttagcc aaaattccgc tgccgaactt tctgtaatgc tcaataagca
     2821 aatcaagaag ttcctgtgcc gactttgact ctgttatcgc tctgattgac gccacatatc
     2881 cgttactgaa tccgatagga ttacctgacg gtgcgtactt aatttgagtt gaaaacagct
     2941 ttctatagat ctgctcaata tcaaacttcg caagtctgta aaggtcgtct ccgataaatt
     3001 tacccgcctg tgcaagacgt gataaaatat tatcgtcatt cgcaagacgt gttgcaacgt
     3061 attcttttat tgcactgctt gtaacgccct ctgttnnnnn nnnnnggtca tcttctattt
     3121 tattaaatat acataattcc aactatgttc accttccaaa tatctatatc tcgtttgatt
     3181 atagcacatt atcaaaagaa ttgcaacagc agaagttacc tcaaatttgt tggaaaaaat
     3241 ctctttttta tattgccaaa catagataag ttgtttataa taatagttaa gagttttatt
     3301 ctttaaataa aaaaacagag ggagaaaatt atggagcaga ttaaaaacgc aattcaaaac
     3361 ggcaaaaccg tactcggtat tgagcttggc tcaactcgta taaaggcagt tttgattgac
     3421 gaaaataaca atcctatcgc gtccggcagt tacgaatggg aaaaccgttt ggaaaacggt
     3481 atttggacat acagtcttga ggacgtttgg acaggtattc agacaagcta caaaaatatg
     3541 gctgacgacg ttaaaaatca atacggcgaa gaacttacaa aaatcggtgc aataggcttc
     3601 agtgctatga tgcacggcta tatggctttt gataagaatg acaagcttct tgtgcc
//
LOCUS       DAWACS010000112         3651 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2746_length_3651_cov_2.736652, whole genome shotgun sequence.
ACCESSION   DAWACS010000112 DAWACS010000000
VERSION     DAWACS010000112.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2746_length_3651_cov_2.736652"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1211 a          635 c          713 g         1092 t
ORIGIN      
        1 tcgcttacga cgccgttgtt tacgacgaaa aacctttgac cttaaaacag tcgtggcgtc
       61 agagagttcg ttggatgcaa ggacattgtg acgttgcatc aagatatttc tttaaactta
      121 tcaaaaaagg cattaaagag agaaaacttt cgtgtattga ctgtgctgta tatcttgttc
      181 agccgataag aattatcgca acgggtatca ttacattctt cgcatatgca caaacatttc
      241 accccgacgg tgacttagga ttcattcagc tttcatacat attcggtaat tcgtggctgt
      301 ggaacgtaat cgttattttg cagttctgct atacaccgtt cgttatatgg tatgaacgcc
      361 gcgaatttaa gccaaaaatg gtattttact acttcacata tattttgtat aacttcactt
      421 gggtgcctat cgcaattcaa ggtatgatcg gtaaaaacaa aaccgaatgg tcacatacaa
      481 aacatactcg cacaatttct atggaagagt taaatcgttt aaacagtaaa tagtatattt
      541 taaaaaccgc tttatgcggt ttttttattg ccccaaacgc ttgacgtatt atttcattta
      601 ttgtataata aatcttatag agagaaaagc agtgcgtggc agtcaaattg tatgatttgc
      661 tattgttttt cggaaaaata attaaatatt taattaatgg aggaagagag taatgttagt
      721 tgaaacaaag gctaaagtag gcgtattttc tattgcatta ggagcttatc tacctcagtt
      781 cccatcattg gtacctgaat ttgaagctca atacgatgcg ttcaagaaaa cacttcctga
      841 cactgtcgaa ataatcgacg gcggaatggt aacaacaaag gagcagtcac aagcagcagg
      901 agatttattc cgtgccgcag acgttgactt ggtatttctt cagttactta catatgcaac
      961 atcatacaat atgctaccgg cagtaaaaga ccttgatgta ccggttgttc ttgtaaatat
     1021 ccaaaagcta aaggctttgg attatgacca cactgatata gcgtcatggc ttggcgaagg
     1081 ttatgcctgc ggtgcagtcg gcgaaatggt tgcagacctt gaaagatacg gcaaaagaca
     1141 cgctgtaatc acaggtgttg ttgaaggcgg tgaccctggt gtacaggctg aaatcgacga
     1201 ttggtgccgt gcagctcagg ttagaagaag attcagagat acaaatatcg ctcaaatcgg
     1261 cagaccttat ccgggtatga tggaccttta cattgacgaa tcaaacctat acagaagaat
     1321 gcacctatat acaaagcagt tcgactggga aaagatgtgg gcaatcgctg acaacatcac
     1381 agacgaagat gcaattcgtg caaaggctca agatattctt gatacattcg acatcgaagg
     1441 cggcggaagt atcgaagaag tttgggatat ggctaaatac gttgttgctt ttgagcaatg
     1501 ggttaaggac gaacatcttg gcttaatcgc atctcactat gacggttacg ctcaaggtaa
     1561 agccggagta cttgacagta tgcttattcc tgcattctca atgcttatta agcaaggtac
     1621 agcttgtgcc gttgagggtg atatgaaggt tgctatggct atgagtatcc taaagactat
     1681 ctcaggtaca ggtcagcttg ctgaaatgta ttcaatcgac tttaacgacg atattgcaat
     1741 catcggtcac agcggttcgg gtgatgctga tatttcagat aaaaaaccta caatgaagat
     1801 tgttaaggta ttccacggta aaacaggcgg cggttacctt actcaattct atccgtacac
     1861 aggtcctgtt acttatcttg caattacaca agacggtgac ggtcacttta agtttatcgt
     1921 tgcagagggc gtaaatgaag aaggtaagat tctttcattc ggtgatacaa atatgagaac
     1981 acgtttcact tgcggagcaa gagaatttgt aaaccgttgg agcgaatgcg gtcctactca
     2041 ccactttgca gccgctacag gcagacatat cgatacaatc ttaaaggttg ctaaaatctt
     2101 taatgttcct gttgacatcg taacaagata aataaaacat ataaaagcaa gctattcacg
     2161 tggcttgctt tttttgtaca tttaatatga aattattgct ttaaagttta atatgtgtta
     2221 gaataaaaaa aggaggtaga gccatatgtc aaaatacgaa gaaaaaataa cagataattc
     2281 actttggtac accgccaccc ctaccccgct tacattgaca ttgccttttt acataaccga
     2341 ggcaggtcat tttcgtgcag aggctgatta taaagtggaa cgtgacgaac acgacagtta
     2401 tcttttgttg tacacaataa aaggcagcgg tacggttgtg agtgacaaag tttctttgac
     2461 cgctcttcct cacaatgccg ttatgataaa ttgtcataat tatcataaat acttttcaaa
     2521 taacgaagaa tgggaattta tatggattca tttaaaaggc agtgcagtgt cggcaatgtt
     2581 tgatgttctc taccctaacg cggtaaatat tatttccgta aaagactttt tgagttttga
     2641 acaacagctg tcggagctta tatgtaacgt gacaaaaaat gatgttctca gcagcatttc
     2701 cacttcttcg caaatacatg atgttctgaa tacacttgtt atttacacaa tcgaaaatga
     2761 acaaaaaagt caaaaacgtc aacattcgga tgatattaat gtggtaattg attttattca
     2821 aaataattac tccgattcaa tatccattga tgatatgata cagaatatcc atatttcaaa
     2881 atatcatttt atccgccttt tccataggat tatgggtaca actccgtata attatttgac
     2941 gaattataga ataaataagt caaaattgct tttgcgtaca acaaataaat caatcgccga
     3001 aatatccgaa gaatgcgggt tccttgatac aagtaacttt atatcacagt ttaaaaagaa
     3061 taccaatcaa aaaccaaccg attatagacg cgattttact tagtttttct ttactcacct
     3121 gctccataga gagtgtgact gtgttatgca aaacgttcga caaatgagac aagaagtacg
     3181 ttagatgttc acaacaagca actgcggaac aggtcagcag ataaatcgca atataaaaaa
     3241 tatatccaat ttttagcaag aaaattccga aggctattta tggttttgcg aatatgaaat
     3301 ataatgatat tatgaggtaa taagatatga ctgctattaa aaataataac actataatca
     3361 tcagaaatac agaacggcaa tcagaggatg tattttttaa taaaattgta attgcatttg
     3421 agaacaatag aaacatttta atcaaaaata aaataatggg accgagtgaa aatgtagtca
     3481 tagctgaatg taacggtgat gaatttaatt taatatatga cattgactac ggaatatatc
     3541 caatagaatg tactgctaac aatgtagatt atgtatttga tatagttaat aatgtaatta
     3601 acattttgtt ataatttgct atactcacaa tgatcacata tctttcgtgc a
//
LOCUS       DAWACS010000113         3621 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2770_length_3621_cov_2.437745, whole genome shotgun sequence.
ACCESSION   DAWACS010000113 DAWACS010000000
VERSION     DAWACS010000113.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2770_length_3621_cov_2.437745"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1387..1396
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1169 a          611 c          822 g         1009 t
ORIGIN      
        1 ttttttctct gaaaatactc aaaaattaca tctatgatgt gatttttgga gctgccggca
       61 ttttgtcgac agactcggtt atatggttat tataacacct aaaagactgc ctttcaagga
      121 caaaaacgaa agttgtattc tgcaaattca aaaaggttat gatttatttg tgcaaaacgt
      181 cacattttta acgaaaaagt aacgtatttc tgatagtaaa tgataaaaaa ataaaaaaat
      241 cattataaat tctttaaaaa aaacaaaaaa tatgttataa tgcacttgtc aataaaatag
      301 aaaacaatgt tacaaaataa cactgtataa aattaaaaaa tgtattggag gatttttacc
      361 atgacagaaa acaagacagt tcttgcatgg cttgaagaaa tgaaagctat gtgtcaacct
      421 gacaatgttg tttggattga cggaagtgaa gaacagcttg aggctctaag agcagaggct
      481 gttgcaacag gtgaaatgat taaactaaat caagaaaagc ttcctggatg ttactatcac
      541 agaacagctg aaaacgacga agctaaagaa actatataca ggttcaatga agggcagaac
      601 aatgtatgtt atcccttatt caatgggtcc tataggttca cctttctcaa agatcggtat
      661 cgagctttca gacagtatct atgttgtact taatatggct attatgacaa gaatcggtca
      721 aaaggttctt gatcagcttg gaaatgacga aaacgctgaa ttcatcaagt gtctacacgc
      781 taagaaagac gtaaatcctg atgacagata catcgttcaa ttcccacagg ataacacaat
      841 ttggtcaatc aactcggctt acggcggtaa cgttcttctt ggtaagaagt gtttcgctct
      901 aagaattgca tcatatcttg gtaagaacca aggttggatg gctgagcata tgctaattct
      961 tggtcttgaa aatcctcaag gcgaagttaa gtatatctgt gctgcattcc catcagcttg
     1021 cggtaagaca aatcttgcaa tgcttattcc accggaatac ctaaagaaca aaggttacaa
     1081 agtatggaca gtcggcgatg atatttcatg gctaaacatc ggtcctgacg gcagactata
     1141 tgctatcaac cctgaagcag gtttcttcgg tgttgctcct ggtacatcag taaagacaaa
     1201 cttcaatgct cttgaatcaa caaagaagaa cacaatcttc acaaacgttg ctcttaacaa
     1261 tgcagacaat acagtttggt gggaaggtct tgacaagaat cctcctgttg atgcaacaga
     1321 atggaagggc tcaaaggtta acggtcctga atactctgca gcaggtaaca atcttgctca
     1381 ccctaannnn nnnnnngatt tacagctccg gctatcaact gcccatgtat ctcatctgaa
     1441 ttcgaaaacc cacagggcgt tcctgtagag gctattgtat tcggtggcag acgtgctaag
     1501 tgtgctccgc ttgtatatca atcaagagat tggcagcacg gtacattcgt tggtgctaca
     1561 atggcatcag aaacaacagc agcagctgca ggtgcagtag gtgttgttag acgtgaccca
     1621 atggctatga aaccgttcgt aggctacaat atggctactt actggggtca ctggcttgaa
     1681 atgggtaaga agcttggcga caaagctccg aaaatcttcc acgttaactg gttcagaaaa
     1741 gatgacgaag gacacttcat gtggcctggt ttcggtgaca atatgcgtgt acttctttgg
     1801 attcttaaca gatgtgcagg taaggttgac gccgttgaaa gcgaaatcgg ttacctacca
     1861 aagcctgaag atatcgacct aacaggtctt gaagacgaaa atgttgatat taagggtctt
     1921 cttacaatcg ataagaaggt atggcttgaa gacgttgcaa atatcgaaga atattttgca
     1981 cagttcggag ataaacttcc gaaggaaatg gctgacgaac ttgctaagtt aaaggctaac
     2041 ctttctaagt aattttaatt acagtgtatt aagaatttaa aagcagacaa aatccttatg
     2101 gttttgtctg ctttttttgt tgacatttat agacacatag gttaaaatgt aagtgtataa
     2161 tatatacaga ttatttatag aaaagaggag aaaatatgaa gaaaaaaggt gtaattatcg
     2221 gtgttataat agcggtaatc gtaattatag cggtaagcat tataggaagt tacaacggac
     2281 ttataagttt agaagaatcg accaacacgg catattccga tatacaggtt caacttcaaa
     2341 gacgtatgga tcttgtaccg aaccttgtta atacggtaaa gggatatgcg gcacccgaaa
     2401 cagaagtgtt tactgccgtt tctgacgctc gtgcaaaact tgccggtgcg ggaacggttg
     2461 aggaggcaag tgaggcaaat gaagaacttt catcggcact tagcagattg attgcaatct
     2521 cggaatcata tccggaatta aagtcaaacg aaaacttctt atcacttcaa gatgaacttg
     2581 caggtacgga aaatcgcata ggtgttgcaa gaaaggatta taatgacgcc gttcaaaaat
     2641 ataacgtaaa aatacgttca ttccctacaa acatatttgc aaatatgctt ggttttgaaa
     2701 agaaacaaca gtttgaggca gatagtgcgg ctcaatctgc tccttctgta agttttgatt
     2761 gatatgagta gggggaagat acgtttcgcg gcattaattt ccgcaatatt tgttttattt
     2821 ataaatattt gtgcgtttgc gaaggttgat ataccgaatc atacggatag attttttata
     2881 aatgactatg cgaatgttat tgacagtgaa acagaggatt atatatttga aaaaggtaag
     2941 gcgtacaatg ctaacggcgg accgcaagtt gttgttttga caatggaaag tatagacgga
     3001 aacgaccttg aagacttttc gattgaaaca gcgagaaaat ggggaatagg tgataaagac
     3061 gcggataacg gagttcttat tctccttgtt atggatagtc gtgatattcg tatcgaagtc
     3121 ggctacggac ttgagggcgt tttaaatgac ggtaagtgcg gtcgatttat cagaaatgca
     3181 acagattctt tgtcggcagg tgattacagc gagggtatta agcagattta tgattcggta
     3241 atcggcgaac tcgaagaccc gacacccgat gaagaagatg atgatgacac tatggttgaa
     3301 ataatgacat atgtagtgtt tttcatagtt ttagcgattg tggtatttat cactaatatc
     3361 aaaggacgtg gcggacgtgg cggctatcat agcggcggcg gttactatgg cggcggattt
     3421 tccagcggaa gcagcagcgg aggaggcgga ttctccggcg gcggcggaag tttcggcggc
     3481 ggcggtgcca gcggtaaatt ttaatattga atcagatgtc gagaaattag ttcggatttt
     3541 gtgaaaatga actcatcggc gtacgttggt atgttagagt ttattttcga aaaaagtgca
     3601 caggcaagcc gtatattgaa g
//
LOCUS       DAWACS010000114         3568 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2811_length_3568_cov_2.652718, whole genome shotgun sequence.
ACCESSION   DAWACS010000114 DAWACS010000000
VERSION     DAWACS010000114.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2811_length_3568_cov_2.652718"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    894..909
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1060 a          657 c          661 g         1174 t
ORIGIN      
        1 agcgagagaa ccgcttttca caggtaaacc cgaaatggaa cgcagcggaa tgggctttac
       61 gataatggaa acgtttatgg acagtatcga agtccaaagt gaagtaggaa aaggcacacg
      121 cattactatg acaaaaaagt taaagtaggt gatgtgttat gcgagataat actgctttga
      181 ttgaacgcgt gcgagagggg gacaagcagg cagaaaacag aatggtggaa gaaaatatgg
      241 gccttgtata cagtattgca agacgatttt taaatcgcgg atatgatgcg gaagatttga
      301 cgcaaatcgg cgcgatagga cttataaagg ccgtaaagaa attcaatccc gaatttaacg
      361 tacaattttc tacatacgca gtaccgatga taacaggtga aataaaacgt tttttgcgtg
      421 atgacggtgc agttaaaata agcagaacgt taaaggaaaa tgcaatgaaa ggctggcggt
      481 gtgaagaact gttaagacga aaactgaaca gacagccgac aataaacgaa atttcaaaag
      541 aaagcggaat tgatgccgaa agtcttatcg aggcgttcga ggcggcaaca ccgcccgaaa
      601 gtatttatga aagtgtttac gataacggcg ataaagaaat tcgcttgctt gatacaatta
      661 ccggtgaaga aattgaggac ggagttataa ataaagttat gattgacgat attttgagcc
      721 gtcttacaga acgtgaaaaa gaaataattc tgcttagata tttcagaggc aaaacacaat
      781 ctgagattgc caagataatc ggagtgtcgc aggtgcagat ttcaagaatt gagaaaaaag
      841 caatagaacg tataagagga gaaatgccat agaaaaacgc actctgtaaa gagnnnnnnn
      901 nnnnnnnnnt ctgttgaaac aggtgcgaca gttgcaatgg gtgtatcggt tgcggtcggt
      961 gtacctgtaa ctgtaggaac atctgtcggt gtcggtgacg caaccggttt tgtcaaaacg
     1021 atttgagata caagtgaaat cggttgactt cctatgttcg agagtgtgtg attatttctg
     1081 taaattaatt tttctatgat aaaatactta gttttatttg ggcagataag caaaattcag
     1141 cttatctgcc tttttgtaat atagcacgga ttttcttccg cgtcaagagc ggtcttgcaa
     1201 aagaccgtat ttttcacact atacaaatgt aaaaatatat gctaaacaag tcgtccatca
     1261 tacataattg acagttctcc gtaaactctg ccccaatttc taagtggcat tgtccacttc
     1321 tttgttgctt caaaggttgc caaatacagt gcctttaaaa gggctgtatc gctcggaaat
     1381 acgctccttt ggctattcaa tcgacgaagt acgctattaa ggctttctat tgcatttgtt
     1441 gtatacataa cttttctgac ttctgctgaa aatttgaaca tcggtgataa aacatcccaa
     1501 ttttcttccc atcttctcat cgcattagga tacttttctt gccatttttt tgtgacactc
     1561 tgcatacgtt cgtatccgct ttcttcgttc ggtgcatggt atatactctt taaatcattt
     1621 gcaaatgctt ttttgtcttt ttccgctaca tatttcaatg tattgcgcac ttgatggaca
     1681 atgcaacgct gatactccgt atttggatag gctgctgcta ttgattcctt tatccctgat
     1741 aaaccgtctg cacataatat taatatatct ctaacaccac gatttttgag ttcatttaaa
     1801 actcctagcc aatattttgc actctcattt tcgccaatgg ttatgcttaa aacttccttc
     1861 ttaccctcgt catttatgcc aagaattatg tatgcagcta tctttttcac tatatgctca
     1921 tcacgaaccg aaaagtggat tgcatcaatg aaaactatcg gatacacagc tgataaaggg
     1981 cgttgttgcc attcctctat ttgaggtaat attttatttg taactgcggt taccatactt
     2041 tcacttacct caaaaccata tatatcttcc acaatatcag aaatttgtct tgttgtcata
     2101 ccttttgccg acatagcaat gattttttgt tctatttctg atatgtcctt ctttcgttta
     2161 gggactatct gcggttcaaa atcaccgtca cggtcttgtg gaacatcaat aggtatctcg
     2221 ccataagaac tacgaagttt tttgtgtttt actccatttc gataatccgg attgtcactg
     2281 cgttcatatt cgtcatagcc taaatgttca tccagttcag cttccatcat ttcctgaata
     2341 gtaccgccta acagatcctt tagtgcctct tgaatatctt ctgctgtctt gatgtcgtat
     2401 tcctgaatca atccggcaat gatattcttc tttccttcac tcattctttc tcgtctacgt
     2461 cttgccataa aaaaattcct cctatgattt atttatattc tatcatagaa ggattatttt
     2521 ttacagactt tttctcacac tctcctatgt tccatacgaa tgctttcagc atttgtgaat
     2581 catcgtgtgc gcttacagaa acaggttctg tgtacggaac agcttttaag cctataagaa
     2641 tgttattttc gtcaaattcg gctacgtaca aaataggtgt atctgttgtt tcgatttttt
     2701 tgtcaacaga tatagtataa gttccgtcat ctttaggtgt gattttcgat tcgtatgcaa
     2761 ctccgttcat taaatttgta gtatactcgg cacacaaatt accgtctttg actgtgagac
     2821 cgaacaaata tgtttttgaa atgtcgggaa tttccgtata ttcatatatt tcttttgctg
     2881 tatttgtttt tgcgtcaaat tcaaaaactt tattcggtga gttaaaatac aattttccgc
     2941 tataagcaac agtaacatta aaatgtgatg aatatgcaga tttattatca ccgtatacgc
     3001 accaataata ttttgaagta tccttataaa ctggtgtaaa agtatttgtc gataaatcta
     3061 aagcggaaag ttcattttta tcattgaaac aatagatttt tccatctaaa aaacttacag
     3121 atcctgatac gttatgaata attgaatctt tataagtgtc atcttctgct gttataaccg
     3181 gttcaccata ccaagtgttt acgttccaag tgcggtgaat atcttgtgct tcaacatatg
     3241 atccgccgtt tgaatttgat accgaagagg ctgaaacagc tttcaattct tcatcgctca
     3301 aaagaaaata tgtgtgattt gcaagtgatg ataaattcgg tgtagggtca tctgaagtta
     3361 aatctaagtt gtaccattta ccgtctactt ttactttgtt ccatatatga ttgcacgcat
     3421 cgctcggaac tgttgtacat tctatttcaa aatatttatt catgatgtac ataaataaat
     3481 atgaatatcc ttggcaaacg catttcttat ctcttactgc cgtgtaaaga ttgcggtttt
     3541 gataagacat atcatattca taatgaga
//
LOCUS       DAWACS010000115         3561 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2816_length_3561_cov_2.227610, whole genome shotgun sequence.
ACCESSION   DAWACS010000115 DAWACS010000000
VERSION     DAWACS010000115.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2816_length_3561_cov_2.227610"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1799..1808
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          996 a          721 c          758 g         1076 t
ORIGIN      
        1 gcttttttga taaaatttgt gtcaaattac accgtaaagc tgatgacgga aacggaatat
       61 ttcgggtatg acataaaaag gctgtattcg gatattacgg tttacattgt acctatggtc
      121 aaccctgccg gagtagatat tgcacttaac ggtcttgata ttactaacga atatcacagg
      181 cacttaatca gtatggtggg aatacatagt tttaatcatg tttggcaggc aaattcgagg
      241 ggagttgata taaaccataa ttttgacgca aagtggcaga tggttgtcga taaaccgtca
      301 ccgtcaaaat acggcggtga atatgcggag tccgagcccg aaacaagggc aattacggaa
      361 tttgtgagaa aagagcagtt tgatatgttg ttggcatttc acagtcaagg ccgtgagata
      421 tattatgact ttgacggtat gacaggtgaa aattcggttg agattgcaaa gaaaatggcg
      481 gaggaaagcg gatatgcggt gtgtattccg acaggtacgg cgtcatacgg cggttgtaag
      541 gattggttta taaaggagtt cggaaaagag ggatttacgg tggaaatagg tacgggacaa
      601 aaccctttgc cgatgagtat gattgatgaa gtgtatgatg aaaatgccaa aattgcttta
      661 tgtgccatgc atgaatgtgc atataactaa aaatgagctg ttgaaaaaac agctcatttt
      721 cagaggataa taaattgtcg tattattttt agtctaattt tgaaccgcac ttatagcaga
      781 aatgagtatt ccgtgcgttt acagagtcgc aaacgggaca agtaatcggc ttaggtgtgt
      841 cattttctgt cggagcgact tgttcggttg tttgtggttg aacaggtggt tgaacaggtg
      901 tttgaaccgg tgcggattgt gcctgtggtg ccgaatatgt tgttgacttt accggagtct
      961 ttgcatattc atagaaacct gcgtgtacaa gaccgaagaa caacggtgca aacaatgtat
     1021 atacaagctg aactatgact gtgataaccg caaacaatat accgacaaac ggaatctttg
     1081 aaagcaatgc aagtatagcc ataacagcat aaatcagcaa gttagggata aattcggcac
     1141 agaacatttg aagtttgtaa ccgtttgtat cctgcattga ttttctgagt gccgcagttg
     1201 cgtttactga tttttcttca ttaagtatat acggtgtgaa tgaatacgca taatactttg
     1261 aaatagcgat aaacggacct gcaatcggaa ctaagaacca aatgaaaatc catagctttt
     1321 tccaacacat accgccggca gttctccaaa aatctttaaa acctttgaaa agctggtcgg
     1381 aataaacttc cttaccgttg aaaccgtcaa gataaactgc cgccatacct gcggaaagaa
     1441 ccgctataac aggcacaata actatcggta atacgccgaa tacaagaacg agtatagtta
     1501 aaagtgaact caaaagtgaa agcccccata gcttaaacgg catttttgac aatacgttaa
     1561 aagctctttt gtagatatta cttataatca taataaaaac ctccttaaaa aattaattgt
     1621 tttgaccgta atatgcgttt gcgccgtgtt ttctgtaata atgcttatcc tgaagctcct
     1681 gtggtgccgg ctttacctgc ggattgatcg attctgtccg gtatgccatt tttgcaacct
     1741 cttccagcac gaccgcattg tgtaccgctt ctctggcatc ctttccccag gtaaagggnn
     1801 nnnnnnnntt tatcctttaa ttcttgaggt gcctcttgaa ctttcggatt aatcatttcg
     1861 gtacgcgccg ccattttcgc aacttcttca agtacgactg cattgtgaac agcctccata
     1921 ccgtctttgc cccaagtgaa aggaccgtga tttttacata gtacggcagg cattccaacg
     1981 taatctttgt ctttgaagaa gtcaacgata agtacgcctg tatttttttc gtatgcctcg
     2041 tcgatttctt cctttgtaag atttcttacg catggaattt cgccgtacat atagtcagcg
     2101 tgagttgtac cgtagcaagg aataccgcgc cccgattgtg cccagcttgt tgcccaaggt
     2161 gagtgtgtat gtacgatacc gccgatatta gggaatgctt tgtaaagctc aatatgagtc
     2221 gctgtgtctg atgatgggtt atatttacct tcgattttgt taccgtcaag gtccataata
     2281 accatatctt caggctttag cttatcgtaa tcaacgcccg acggcttgat tgcaaaatag
     2341 cctgtttctc tgtcaatttc acttacgtta ccccaagtga atgtaacaag tccatgcttt
     2401 ggcaactgca tattagcctc gtaaactctt tgctttaatt cttctaacat attatatatc
     2461 tccttttatt agtatttcaa atattcaact gccgcttttt cgataggcag accgttcttg
     2521 taacgtacag caaattcgtt aaatccttta acgtcctcgt ctgtcggctc aagtgtgtat
     2581 gattcacttt gtgagaatac tttgttgtta aggaaatcgg taagtgtttc gttttctgat
     2641 tttgcaagca tatatgacgc aagcaatgcg ataccccaag caccgccctc accggctgtt
     2701 tccataagtg aaacaggtgt gtttatagcg gcagccataa acttctgacc aacgtccttt
     2761 gtcttgaaca acccgccgtg accgaacatt ctgtcaagct tgacattttc cttgtcaaga
     2821 agaatatcca taccgagctt taatgttccg agtgcggaat aaaggtgagt tctcatcata
     2881 tttgcaaggt taaacttact gtccggtgta cgaacgaaaa gctgacggcc ttcttcaaga
     2941 cctgtaatat tttcgcccga gaaatatccg tatgaaagaa gtcctccgca gtctgcgtca
     3001 ccttcaagag cttttctgta aagtgtgccg tatagctttt ccgtacttat ttcaataccg
     3061 agttcggctg aatattctct gaatatattt acccaagcat taaggtcaga tgtacagttg
     3121 tttacgtgta ccattgcaac gtcgtcaccg accggtgttg taactatatc aatttcggga
     3181 taagcctttg aaagcggttt ttcaagtaca accatagcaa atacggacgt gccggctgaa
     3241 acgtttcctg tacgttttgt aatgctgttt gttgcggtca tacccgtacc ggcgtcaccc
     3301 tccggagggc atagcggaat acctgcactg agtttgcctg ttgtgtcaag aagttttgca
     3361 ccctcttctg aaagcgtacc ggcattttct cctgcaacaa gcacttttgg aagtaagtct
     3421 gaaattgtat aaggaagatt tttatttgaa atcaacttat cgaatttctc aatcataccc
     3481 ttattataat cttttgatgc actgtcgata gggaacatac ccgacgcctc gccgataccg
     3541 agtacaaatt cacctgtaag t
//
LOCUS       DAWACS010000116         3525 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2847_length_3525_cov_2.271470, whole genome shotgun sequence.
ACCESSION   DAWACS010000116 DAWACS010000000
VERSION     DAWACS010000116.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2847_length_3525_cov_2.271470"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1439..1448
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1105 a          778 c          614 g         1018 t
ORIGIN      
        1 gtcacataca tttcgcacct cggcaatctc caaccgccga gttttttgca agccttgttt
       61 atcgctataa tttcggcgtg aagtaatgcg tttttcttgg tttcacgctt gttgtatcct
      121 cttgctataa tctcaccgtc acgcactata accgcaccga ttggagtttc accgattgac
      181 gcggcttttt ttgcttgctt cagtgcctct ttcataaatt tttcttccat acaattctcc
      241 attcagtaga tataacctta ttttatcaca cctcttgaca tatgtcaaaa atagtagtaa
      301 aatgtgaaaa atgattgtaa ttctaaaaca aaacgcagaa caaaacgagg taaacgcact
      361 tttaaagcaa ctggaggaga tgggctttga ccatcattac agtaagggcg aaaattccgc
      421 aatagttggt attatcggtg atacttcaac tcttgacaca gacgatttaa agactatcga
      481 cgtagtcgca gacgttaaga gagtttccga gccgttcaag cttgcaaacc gcaagttcca
      541 cccggataac agtattttca acattgccgg cagaacagta ggtgaaggtc atttcagcgt
      601 cattgcaggt ccttgttcgg tagaaacagt tccgcaaatg acagaaacag ccgaagcagt
      661 taaaaagagc ggtgcttcat ttttaagagg cggtgcattc aagccgagaa catcacctta
      721 ttcattccaa ggacttcatg acgaaggtct taaaattctt cttgaggcaa agaaggcaac
      781 aggacttccg atagttacag aaattatgaa tcaggcacat cttgatttgt ttgaagatgt
      841 tgatataatt caagtcggtg caagaaatat gcagaacttt gaacttttaa aggaacttgg
      901 taaatgcaat aagcctatac ttttaaagcg tggtctttca agcacaatcg aagaatgggt
      961 tatgagtgcg gaatacagta tggcaggcgg taacgaacag gatattttct gcgaaagagg
     1021 tatcagaact tatgaaacat tcacaagaaa tactcttgac ctttcggcaa taccggcact
     1081 aaagtcaatt tcacacttgc ctgtaatcgt tgacgcaagt cacgcaacag gtttgccgtg
     1141 gatggttgaa tcgcttacaa aagcggctat cgcagtaggt gcagacggta ttatgattga
     1201 agttcataat aatccaaaga aagcattgtg cgacggtgct caatcactta ctccgcagca
     1261 gtttgacaat gttatgcaga cagttaagaa gcgtgttgag tttgaaggta aaattttagg
     1321 ttaatttaaa ggcagtcgat tgactgcctt tttgtttgca aattttcaga taacagcacc
     1381 accgcatttg tttgcaattt tgtatgtagc catattttat ttttgatttt gcaagtagnn
     1441 nnnnnnnntc cgaaattctc ataaacgata gcaaacttaa tcacacaaaa agggcgacca
     1501 actgtcgccc ctactacaca actattcatc tttatgaaag tgtctgtaaa tcgcataaac
     1561 caaagcaccg actgacaaaa cagctatgta tatcgcaaaa gcaacaaaga caatactacc
     1621 tgcttttgtg ggcggtgtat atatatatct gcgataacca atcacatctt tattcactgc
     1681 caaagttacc gttgcaaaag caatcgtaac caaagtcagc agcccgccta tataatactt
     1741 tttcataaat cccccttatc ttaccaacaa atacaccgta tatgccacat acataaaaat
     1801 cataaccaca ccgcaagggc gattaatttt cttattagca agtgcaaaaa tataagtaaa
     1861 caagcatatt ccaatcaaca cgcaaccgtc aattaccaca ttaaagtcag tacctattgt
     1921 accgattgtc gctgatattc cgagaatgaa caacaaatta aatatattag agccgattac
     1981 gttacctacc gcaatatcat tttgttgttt tcttgcggca acaatcgaag tcacaagttc
     2041 aggaagtgat gtacctattg ccacaacggt aagaccgaca actctttcac tcattcccgc
     2101 cataagtgca agttcctttg cgcctttaac agtaagctga ccgccgacca caacaccgac
     2161 aaagccgatt attataaaca ataggcattt ccacattggc aaatctgcct ttttgctatc
     2221 ctccgcactt tcaattaatt tactttcttt cttacccatc acaacagaac cgaccataaa
     2281 caccgcaaac aatgcaagaa gtataatacc gtccaatctt gtcaatgcaa tatcaccatg
     2341 atttaatgtc attaaaagca caatcattac agcggttata ccgatacaga atggaaaatc
     2401 acgttttagt atatttcctt ttaccgcaac aggtgcgaac aaagcacttg caccaagtac
     2461 gacaagcgta ttaaacatat ttgatccaat tatattaccg attgcaattt cattactgcc
     2521 ttgcagtgac gcagttatgc taaccgccgc ctcgggcaaa cttgttccga acgccactac
     2581 cgtaagtccg actatatatg ccggaattct caactttgcc gacaaatcac acgcaccgtc
     2641 aacaaacaaa tccgcacctt taaccaaaag cacaaaccct atcaccaaaa ataaaatcat
     2701 taaaaccatt aaatataagc tccttttcct ctgtaattcc tcataaaagc aaagtagaca
     2761 aaacccatat gggttttgtc tacaattaaa ttgtctaatc gaattagtga acgatagcca
     2821 tttctgtatc cttatcaaag atatgaattt tgtttgtttc aaatgcaacc ttgattgtat
     2881 caccaatctt agctgttgaa cgttgattaa ctcttgctgt gaactgttca cctgcaagtt
     2941 caagatacaa atatgtttca gcacccatca tttcagtaac ttcaacgtat gcattacata
     3001 cagcctcagg tctgcttgaa atgaatgcct cgtcatcgtg aatatcttca ggtctgatac
     3061 caacgattac ttccttaccg atataacctt caagttcagg tgtaacctta ccgttaggaa
     3121 tcataatctt gttatcgcca aatgatgcgt atacgctgcc gttttccttt gatagtgtag
     3181 cagtaacgaa gttcatttgc ggtgaaccga taaatcctgc aacgaatacg ttatcaggct
     3241 ttgtataaag gttagcaggt gtatcaactt gttgaataac accgtccttc ataacaacga
     3301 tacgagtacc cattgtcata gcctctgtct ggtcatgagt aacgtacata aatgttgttt
     3361 gaagtttgtt atgtaacttc ttaatttcag ttctcattgc aactcttagc tttgcatcaa
     3421 ggtttgatag aggttcgtcc ataaggaata ccttaggatt acgaacgatt gcacgtccca
     3481 tagcaacacg ctgtctctgt ccaccggaaa gagccttcgg tttac
//
LOCUS       DAWACS010000117         3513 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2856_length_3513_cov_2.290052, whole genome shotgun sequence.
ACCESSION   DAWACS010000117 DAWACS010000000
VERSION     DAWACS010000117.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2856_length_3513_cov_2.290052"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    633..642
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1259..1268
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          890 a          842 c          619 g         1142 t
ORIGIN      
        1 atctctcgga ctgatactta catcgcctct tgcagaaaga acatcaaaca aagcgaattg
       61 tccgcttagc agaagcgtta taggcggtac ggatgaggac ggataaatcg tttcaaacag
      121 attatctata ccgccgcctg cacctttaat ccaaaatacg cccattccgt caacatttat
      181 gcccggtgta aatccgcctg catagaaata taatttatcc ggaataggta ttccgttgtt
      241 acttctaaga cgaatttccg cctccatttc aaatgccatc atatcggcac tgccttgaac
      301 gccgatagtg tattcgttgt tcataacctt taagtcaaga gtaccctcaa cgctcggaag
      361 tccatccgca tatgacggaa cttctacttc gacagacgta ttaaatccga taaatccacc
      421 gccgaaaagt atatcatgga tatacaaatt cagacttccg ccctctgcgt cttcccattc
      481 ctcttggtcc tcggcatatc tgtcctgtac atctctaagc tgtgacggag tgtaattact
      541 gctcgccatt ttcagttgtg caacctccat tgcggaagtt tttctgttgc tccaatcaaa
      601 gtttgacgga acaagaaaat caggtgaaag ttnnnnnnnn nnaatgcgat tactctttgt
      661 ttcggtgttt ttgaagtgac ttcaccgtca actgtcgcca tctgtccgaa ctgacagtat
      721 ctgaattcca tcaccattcc cgcaatggtt tgtgcggtac ttgccgcacc cggccacata
      781 agcataatac cgtttgtatt tgcaacgctg ttttcaacgt cacctgtctg ttcgccctcg
      841 tttgtgtact gcaaaagtgt tgattcggag ccgttttcaa atgacgaaat cgcacataca
      901 ccgctccaaa cctttgttct tgcgcctgtc gtataaactt cgccgtctat atctatatta
      961 atagtttggt cgtcctgacc gatgttttcg acagacacgg ttacagtacc tttttcaata
     1021 tcaagacaat tactgatatt tattgtgctt ttcgccttat ggtctaccgt ttcaagcgaa
     1081 actgcctctg cctctgcaat tttaccgttt tcatatttta acgtaaagtc accgcgaagt
     1141 tcaagcaaag agatattttg agcatttgga acttcgtcac tgtattcctt ttcgtcttta
     1201 tacgccctta ggacatactt gttatcgccg tctttttcaa tcgtgacaac tccataagnn
     1261 nnnnnnnncg ccttgatatg caatatcatc actgacatta aacttcagtg cgtcagacgt
     1321 aatatctttt ttagtcggat ctttcatatc aaatacaatc tgatatgtac cgaccgacat
     1381 aagctcgtca agtatcaaat ccgccgtatt gttttcggta tccaaaatga agttttccgc
     1441 acttacggtc aacggctgac tgccgtttaa aggcgtaacc tttatatcgt attcactctt
     1501 atttttaagt aaattgaaat tctgacctgc aaggtaaata tgtcttgttc ctctgttata
     1561 aacaatctcg ggtgcggtgc ttataaacga caccctgtca cctgttgcac ccgggcaaag
     1621 aagatatata cttctctgac cgattaagtt ttcggaaagc aatttaccgt ccgttccgct
     1681 cacgtcataa caacgcactt cgatttttct gtaatcggta gccgtaagcg gttcggcatt
     1741 aaagttaaac acaacctgtc gttcttcatc tgcgttaaaa tctataatat caacactgaa
     1801 agggtttgaa tatgaagctg taacgtcaag attacccgac aagtcatacg gtgttattcc
     1861 ctcctgcgga tataccgcaa cggcaatttg tttcatttcg ttctgcgaca cgtttttaat
     1921 ttgcgttgac acgctgaaat caccgttttt accgcctgcc gtttgcggtt tgtattcgtc
     1981 ttttgtatcg gtaagctgca ttgctccgag ttcagatgaa aaatttattg caaccttatc
     2041 atcgttattt acggttacgt ttgaatatat accgtaattt gtcgcaacgg tatcgccctc
     2101 accgtttgcc gccacactgc ccatatcgaa atacaaagca tacgcactgt cggcagtcaa
     2161 atatttttcg ttatacggat ttgtaaaagt caacgagtta tccggctcaa aatcgaatac
     2221 acttgacgca aggttgttcc aatgtccgaa cgctatttga tacggtgcga caattttatt
     2281 gttaatcgac gcattcaccg tataagccgt tacagacggt gctttcggat tgtcatatcc
     2341 gaacaatgcg ttgttgtatg cttctccgtc agagttatca ataaccgttt cggattgtat
     2401 ctgctcatat gtactgtcct ttttcgtcag ttcatacacc gcataatcct gataaccgag
     2461 tgcggtatca agcataactc ttgcctgaac actgtccgca cttcttccgt ccgtactctt
     2521 tacaccgtat gaaattgacg ccataccgtg cagagccgat tttgtatttg caagcgtgac
     2581 ggtctgcgta aaagtcaaac cgtcaacgct ccacgttgcg acaatgttgt cactgttttt
     2641 caccaaagtg acgtcactac tgtccttacc caagaaaccg tattttcttc cgaaaatata
     2701 gtcctttacc tcatcacctc tttttacacg aaaagaggta tatgatgtat cataattttc
     2761 atcgggaaac aaaagatatt tattatcatc tgctttgttc agcttatctc cctcaacggt
     2821 atctatcaga aaaccgccgt ttttcgctga aacaacgact ttcaaataac cgtcgttcaa
     2881 ttcttcgtaa tccgccttat tcagattttc ggcaaacgcg ccgataggca ttataccgaa
     2941 aatcatcgct gttacggtta aggctgatat aatctttttt aatctttgca gcatagtaaa
     3001 cctccttttt tcattattga acgtaaatta caaatctgta attgtcacgt ccctgcgttt
     3061 gtacattaaa tgtataatac cccgcctttt tcggaacgaa tgtaatcggc tctgtattat
     3121 ctttattctt cgcccattca gtcgcaccga ttttcatttg accgaccgtc tttataccgt
     3181 ttttatattt aacactgtac ggctcgccat tgctttcaac gacaatgttc tgtgcctcgt
     3241 ttttgcttag cacaatcata tctctgtaaa tatactcacc gtttacgctg actctaagct
     3301 gagtatcctc atagaaatag atatatccgt atctttcact gctgtttcct gcctcgtcag
     3361 ttgcggtata agtcaccctt acggttcccg ttgtctgcgg ttttgtgtaa gtaacatcaa
     3421 gcttgatttt gtccttttcg gtttcgttgt cgcttaccgt catattcgct ttaagctgtg
     3481 acaaaatatc ttctgaattt aagtcgattt cca
//
LOCUS       DAWACS010000118         3437 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2923_length_3437_cov_2.289474, whole genome shotgun sequence.
ACCESSION   DAWACS010000118 DAWACS010000000
VERSION     DAWACS010000118.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2923_length_3437_cov_2.289474"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1996..2005
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1109 a          655 c          723 g          940 t
ORIGIN      
        1 cccttaaatt ttatcatatc ataaattgat aaaaattgat aattaaataa cagtgaattt
       61 agtttaatat gacgataaaa tttgtaatga aaatataatg aattttgtaa agaataatgg
      121 ttgaaaactg ttagtaatgt atggtataat gataaactga tatagagtat ttaaagtcag
      181 aagggatttt taatatgaag aaaaaaatca tggcggctgt tttatgcact tcgatgctga
      241 ttcagacagt gtgcgtcaat gcggaaacaa gtgaaagcga cataaaaagt gctatagaaa
      301 aagcaataga atggaaagct gataaggata gtccgtttta cggaatcgga tcatacagtt
      361 ctgattttta tattatggct ttgaaccgaa tgggtaaaaa ctatgattac agcagatatt
      421 tggctggtct tgacggagtt gcggcaggtt acggcgaaga acataatgcc tcgtctatgc
      481 aaagaacagt gcttgcaacg atagcgtcag gcggtgatcc gagaaacgtc ggcggacgtg
      541 acttggttgc ggacggtatt tattacagag ataatgtttc accactcgga aaagagggtg
      601 cagacggata ttcgtgggga cttattgctc ttgattcaaa gagttttgaa actcctgaat
      661 gggcattgtc aaacagaaac gatatacttg ccgggatatt gtcacatcaa aatacgaacg
      721 gcagttttga tgacagcgtg tacagtaccg cagttgcaat tacggcgctt gcaccttatt
      781 acgaaacaag cggtgcgtat acgataacgc aaaatcagac gggttatgtg attgatttaa
      841 gccctaaaga cgcggttgag gatgcactta attatttgtc gacagaacag gacaaggacg
      901 gtgattgggg tgaccttgaa tcaacggcaa tgactgttat cgcacttgat acgttggggg
      961 ttaatgctga cagtgacggc agatttgttg ctaaaaacgg cagtgcggta gacggcttaa
     1021 tgcagtatca gcaaaaagac ggtggatttt cgaccggtaa gggaaagagt aacagtgagg
     1081 caacgtcttt ggcagtgtgt gcacttacaa gccatttgag aaagttgcag aataaagcga
     1141 cattctttaa ttttgacgtg aatgattcgg ttgtgtttga aacacctgca ccgacaaaag
     1201 cacaatcttc aggcaatacg tcaaataaga catcatcaag tacatcaaaa tcaaataaga
     1261 caacaaagac aacaacgaaa ccgaaaacaa caacaaagcc taaaacgaca acaaaaccaa
     1321 agacaacgac taaggtgacg ggaacaatga aaccgacaaa aacggcttcg cctatggaat
     1381 catcatcacc aaagccgtcc ctgacaccaa gaccgacaaa aaagccggca cttgtaggac
     1441 cggttgagat gcctggacca atgccgtcat ttgaaccgaa tgaagcactt ccgcaggaaa
     1501 agggacatca cgacagaaag agcggaggag cggcagttgc gattgtaagc atagtagcgg
     1561 tactttgctt ggcggcaata gcggttgtat tgtatctcgg taaaacaaag aaatttgagg
     1621 gtaaaggtat atttaagtat cttgcaccga aaaagaaaaa gaagaacgaa atatataagg
     1681 caaaaccgca tagaaggaca gaacaacaca gacgttatga agaacgtgaa aagtttaaaa
     1741 aacgacgtaa atttgataaa cgaagaagat aaataaaagg tgactgcaaa aaatgcagtc
     1801 accttttaca ttgcagacaa ccttttcagc cttccatatg cttgcctaaa aaaccggcgg
     1861 cggattctgc taatttttct tcaacttcag acgcagtttc accctctttt aaaatgaaca
     1921 aaacagagcc gatcgtgtca ccctccgata ctatcggaac aacaacgccc gcgttgtatt
     1981 tttcaacacc gtcagnnnnn nnnnngaaac gggattgccg tccttgtagc ttacaatgat
     2041 tttttcgttc attacatttt caagctcact gcttacaggc ttttcgataa gctctttctt
     2101 cggtaatccc gatactgcaa tgacattgtc acggtcggtt atacaagcac cgtgtccgct
     2161 tgctttggca agtgtttcaa cataattagc ggcaaagtca ccaagctcgc cgattgggga
     2221 gtattttttg aaaataacct caccgtcttt ttctgtataa atttcaagag gatcgccctc
     2281 tctgattcgc atagtgcgtc gtatttcctt tggaataaca actcttccga gatcatctat
     2341 tcttcttaca attcctgttg ctttcatcgc ttttcctcca aaataaattt atatttgtgt
     2401 taaatattat ttgcattgtt tttttgattt atccataatt gataaaaaaa acaaaaattt
     2461 tggagtttta aaaattaatg tgtaatattg ccgctgctgt atgccacttg aggggagcag
     2521 tcgtgaaacg gcagaggtgt ggcaaatgag caaaataaaa gggcgaccat tggtcgcccc
     2581 taagaaagta cattaaataa tcatatcatc acggatcaag gtggtgaata tctcttggga
     2641 acaagcttgc ctcacggata tttgcaagac caagcatttt cataacgaca cgttcaagac
     2701 cgatacctag tccgccgtgc ggtggcatac cgtacttaaa tatatcggta tatgcaccga
     2761 ggtcgccctc ctccatatga taacgtttca ttttttcaag aagttcgtta taatcgtgaa
     2821 ttctctgacc gcctgttgtg atttcaagac ctctgaaaag caagtcaaaa ctttagggtc
     2881 gtctgccgaa tccattgcat agaacggacg ttttaatccc gggaactttt caacgaatat
     2941 aaattcactg tcataattct tttttgcgta ttcgcacaat ttaacttcat ctgtaggatc
     3001 aaggtcaaat tgattatgtg acttgccgag aatatcaagt gcctcaaaga atgttaccga
     3061 aggaatttca ttgataacag gcaatgttgc acctaaaagc tccaactcgt cactgtaatg
     3121 tttcggcaaa tactcaacaa catatttaag catagccgtt tccatagcca taacatcatg
     3181 catatcgttg ataaatccca tttcaaaatc aagtccgata tattcgttca aatgacgtgt
     3241 agagttgtgt ttttcggctc tgtaaacagg agcaatttca aacactctgt caaagaatgc
     3301 tacgcaagtc tgtttgtaga actgcggact ttgtgcaaga tatgcgtctt tatcaaagta
     3361 tttcaatttg aatatattag caccgccctc agcacctgcc gcaacgattt tgggtgaatg
     3421 tatttcggta aagttat
//
LOCUS       DAWACS010000119         3413 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2944_length_3413_cov_1.987493, whole genome shotgun sequence.
ACCESSION   DAWACS010000119 DAWACS010000000
VERSION     DAWACS010000119.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2944_length_3413_cov_1.987493"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    215..224
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1910..1919
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1063 a          573 c          793 g          964 t
ORIGIN      
        1 tttatgtccc tgtgtattgt agtcattcgt ttgcgtgtcg agtcgaaagg tatttcggca
       61 atacgtctgt attttttgtc aagtacggac ttgtcaaaac cgtgtttttt tgcggcggca
      121 agtattgcac tttctgtcgg acttttgtgt ccctcagcaa agtcgcagca catcgaacaa
      181 agtcgtatca gctttttttc atcgtacgta taaannnnnn nnnnattttg ttttgcgtta
      241 aagtacctgt tttgtcggag catattacgg atgcactgcc gagcgtttcg acagacggaa
      301 ggtttcgtac tattgcgtta tgctttgaca tacgcattac gccgattgca agcataatcg
      361 taactattgc cggaagtccc tccggtattg cggcaaccgc caaagaaacg gctgtcataa
      421 acatatcaaa cggcggaaga tgacggaaca gtccgacaat aaaaataaca aggcatacga
      481 aaagtgcggc aataccgagc gttttgcctg tatcggcaag ctttagctga agcggtgtct
      541 gcggtgtttc atcatcaagg agcatattcg caatgtgtcc gacttccgta ttcattcccg
      601 tacccgtaac tatcgcggtg gcttttccgc ctgttacgga ggtggaagaa aggattatat
      661 tttttctgtc cccaagtgcc gtgaattctt caagtacaac cgatgcgtct ttttcggcgt
      721 ttacagattc accggtaaga gaggattcgt ctgttgtgag gttgttagat gtgacaagac
      781 ggcagtctgc ggcaacaaga tcgcctgccg atattataag tatatcaccg ggaacaacgt
      841 tcgcggcagg aatattaagc tcattgccgt ttctgagtac acaggcatgg ggtgatgata
      901 gtttctttaa agcctcaaga cttttttcgg cacgtttttc ttgaataact ccgagcaatg
      961 cgtttaatat gacgattgca agaattatta caggttctgt tatatctgcg tcaccttgca
     1021 taattgacgt tataaatgat accgcagcgg cggaaagaag tataattatc ataaaatcat
     1081 taaattgttc aaggaatttt ataaatatgt tctttttctt tttttcgata agttcgtttt
     1141 tgccgtaatg gtgaagtcgt ttctttacct cgtcatgtgt aagtcctgtt tttatatccg
     1201 taccgagcaa ttcggcggtt tgctgtacgg ttttattgtg gtatgtttgc atttttatac
     1261 ctcctctgtt ttatacgata taactattcc gtattgcctg tatttataac caacttgtct
     1321 ttaaattgta atcaaaatgt tttagtgtcc cctccgaaaa tgtgttataa tgtaagaaaa
     1381 ggagggacac taaatggcaa gaaaggcaag agagataagt aaaagcggta attattatat
     1441 tacgctgaaa ggcaatgaac tttttgtgac agacgaggat aaaaaaatgt ttgttgaaat
     1501 tcttgaaaag aatttcgcaa cgggtattgt acatagctat tatcttacaa agtcggaaat
     1561 tcgtcttgtt gtaaaagagg gtgaaaaggg aatttcaatg acaatgaaac ctgtcacgac
     1621 aagctatgcg agatatttca acagaactca cgaaagagag ggcaagcttt ttgcggacag
     1681 atttaaaagt gagcctcttg aaactgacga agaaatagaa gaatgtatca aaaatttaga
     1741 aaacgataca ccgaaaaagc aggcaaaacc taagagaacc gcaccgaaga aaaaggacgt
     1801 gacaaaggtt aaaaagactg aaatcaaaat cgagaaaaca gaagaaacaa agccaatcga
     1861 ggaaaagaaa gaagaaaaga aaaataaact tccgtcatgg cttttgtgan nnnnnnnnng
     1921 cagatatatt ttaggaggtc ggcattttga atttttatgt tattaaatta aagcatatta
     1981 taatagcatt actgattttt gcggttatac cgataattag gtttacgact tcaagagcgg
     2041 taagcgtgtt taatgtaaac ggcagagaaa ttccgattta cagcgtggag agggacgaca
     2101 ataaaattgc actgacattt gactgtgcgt ggaacgatga tgatattgac tcaattcttg
     2161 atacgttgga caagtataat tgcaaggcga catttttcgt tgtgggtgat tgggctgaaa
     2221 agtattccga aagtttgaag aaaatttatg acaggggaca tgaaatcggc aatcattcat
     2281 ataatcacgc ggattatacg aaaatgtcgg cagaggcgat tacagccgac cttgataaat
     2341 gtgacggaat tgttgaaagc ataaccggtg aaagaccgta ccttatgcgt gcgccgtcag
     2401 gaggatataa taacactgtc gtgaaagcgg cggaggatag cggaagaatg tatattcagt
     2461 ggtcggtgga cggaattgat tacggtgacg cggagacaca ggatatatat gaaagaagtg
     2521 tcagacgcac gcaaaacggc gacataattc ttttgcataa cggcacaaag aatacggcgg
     2581 caattctgcc gaaaattttg gaggcattgg agtgcaaata tgaatttgta accgtgtcgg
     2641 aacttattta caaagataat tatgtgatag acaatacagg cagacaattt caaaaataat
     2701 tatttacaaa accgttgtaa tctattataa tagataatat aaaagcgaaa cggagaaaat
     2761 aatatggata agaaatatga atataacagt cctttggtgt atcagcgggc agatccgttt
     2821 gtgtatctgc atactgatgg atattactat ttcacaggta caatgccaca gtatgactgt
     2881 attgagataa gacgtgcaaa gactcttaac ggacttttga cagttgattc ggaagtagtg
     2941 tggaaaaagc atccgacagg tgaaatgggc tcacatattt gggcgcctga acttcattat
     3001 atagacggtg tgtggtatat ttatttcacc gccggtgacg caaaagacgt atgggaaatc
     3061 agaccgtatc tgcttatgtg cgaggacgaa aatcctatga cgggtaagtg gattgaaaag
     3121 ggcagaattg atgtgggagc ggaaagcttt tcgcttgata tgaccacatt tatacataag
     3181 ggtgaacaat atgtactttg ggcgcaaaag acagagcctg cagatgtatc tgacgtgtat
     3241 attgcaaaaa tgaaaaatcc ttggactctt gcaacaaagc cgatgattct tacaacaccg
     3301 gagtatgatt gggaagtgca gggatttaaa gtgaacgaag gcccggcggt acttgtcaga
     3361 aacggtaaag ttatcgttac atattcggcg tccgatacag gttggagata ctg
//
LOCUS       DAWACS010000120         3368 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_2996_length_3368_cov_2.145487, whole genome shotgun sequence.
ACCESSION   DAWACS010000120 DAWACS010000000
VERSION     DAWACS010000120.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2996_length_3368_cov_2.145487"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1071 a          610 c          731 g          956 t
ORIGIN      
        1 ccctcaaagg ctctgtttga tgtttgaaaa cctgccatat agaaatatcc gagttttttt
       61 gtatcaagga attttttgtt gccgtcctca aatttttcag cttcaagttc ttctgtcgca
      121 aatgacttta ccactcttat accgagcaat gtatcctcaa cctgtgaatt aatttcgccg
      181 atttggttac gctgtttctt gaacgcttta cgcatacgcg tattaaagaa ttttacaaaa
      241 cagaatataa gcggaagtga tgcgaacaca ataaccgtaa gcggtacgtt tactccgcaa
      301 agaattataa atgaaacgat tattttcagt gcggcgatga aaaattcttc ggggcagtgg
      361 tgtgcaaatt ctgttatatc aaacaagtca cttgttattc gtgccataat ttgtccgact
      421 ttgtgttcgg caaagtatga atgtgaaagc gtttcaaggt gtgcgaataa gtcacggcgc
      481 atatctgttt cgatttttgc tcccataata tgtcccgtgt cagccatata gaaatttgcc
      541 gcagagtcga ttattcttaa aatcagatag aaaagtccta tttttaagat tattccgacg
      601 gttaatcctg ctatgttagt gatacccata tttgttatgt aacgcacgag cattgggaat
      661 acaagctcac aaaccgttgt cagtgacgca cagattagat cgagtatcag tatccatttg
      721 tattttttga aatacggtac aaagcgcttt ataagataac tcatcttcat ttgtttttct
      781 tctgtctgca ttgttttact cctttataaa aattcacata gtaaaaaaag aatgcggatt
      841 aaaaatcagc attctctttg gcggagaagg tgggattcga acccacgtgc ccgttaaggc
      901 aaacgcattt cgagtgcgcc ccgttatgac cgcttcgata cttctccaaa agtaccttaa
      961 tattttacca tagtaaaaca aaaaagtcaa gcgaaaaacg aggtttaaat tgactttata
     1021 aaaattgagt gatatactga aattaaagca atttgaaaaa cggagctatg gttatgaaaa
     1081 ggtttatata tatttttttt gccacgttat tggctatgat gtggctgggg attaatgttt
     1141 atgcagatga atatgacggg ttggattttg attacggaag tgtgcttgtg cagttaaaac
     1201 cgcagattac cgatgctgtt tcaatgtgtt cagacccgtt tgaagaattg aatatcagtg
     1261 atgcaaaatg tattttcggt gacgatgcaa gcagtatcag tttgttttct gataaaacag
     1321 agcctattat atatgtactt gatttggaaa atccgagccg tgaaaatgtt atagatacga
     1381 ttgaaaagtt aaaggctatg ccgaatagtg aatatgcaga gcctaactac aatgtatatg
     1441 agttttccga acctaatgac acgtactatc aaaacggaaa acaagctgtg cttgatttaa
     1501 tcggtgcaaa aaagctttgg gattttgata ttgattgctc tgatgtggct gtcggagtta
     1561 tggattcggg tatacagaca aatcacgagg atttaattga taatatatgg gtgaatacgg
     1621 gagaaattgt cggtgacggc attgataatg acggtaacgg ctatattgat gacatatatg
     1681 gttggaattg cggcgacagt aacggtgatg tgtcatatgt gaatgaacac ggtgttcacg
     1741 ttgctggtat tgtaagtgcg gcgactaata attccaaggg tgtggcgtct gttgcacgta
     1801 atgccaaaat cgcatcaata aagattttta acagcagtgg taaaagtact ttatcttata
     1861 taatagaagg gattaattac gcgaaaaaga atgatataaa tattattaat tgcagttttg
     1921 gcggtgcagg ttggggaagt acttcggtga gtatcgttaa atcggcgatt gaggcggtgc
     1981 cggatatatt ctttgttatt gcggcgggaa atattgcgac aagtacaccg caagcggata
     2041 atgacaaagt ggcagtgtat ccgtcacaac ttacaaaaga cctcgacaat gtaatttctg
     2101 ttgcaaacac gacaagcagt gatgaactca gcagtacgtc gcattacggt gcaacaagtg
     2161 ttgatattgc agcaccgggt acagttattt acagtacaat tccgacaagc agttacggaa
     2221 caatgtcggg aacttctatg gctacaccta tggttgcaag tgctgttgcg gtaatgcgtg
     2281 cagtaaatcc gaatatttcg gcaaaagaaa ttaaagaaac tttgtgttcg agttcagaca
     2341 agctttcggc gcttacaggt aaagttataa gcggcggaag attgaatgcg tataatgcgg
     2401 ttaaggcaat tatgccgaca gcgacaccaa cggcaacacc aacggtaaaa ccgacagcaa
     2461 cgcctacaac agtaccgaca gcgacaccaa cggcaacgcc tacgacagta ccgacgccga
     2521 cagcgacacc gagtccaacg gcaacaccaa cggtaaaacc aacagcaacg cctacgacag
     2581 taacaccaag tccaacggca acaccaacgg taaaagcaac agcaacacca acgatagtac
     2641 cgacagtaac accaagtcca acggcaatgc ctacgacagt accgatgccg acagtaacgt
     2701 caagtccaac ggcaacacca acggctggtc cgacagtcac accaatgccg actgttttaa
     2761 cgaacaataa aattgagtgt gaaataaaag ataacaaact tatcgtatcg cttaattttg
     2821 aagaagatga cgaagtgatg tatgttgcgt tcagaaaagg taatgaactt aaaaagattg
     2881 tgacacctga catacataat atgacggcgg aggtcgattt gaatgatgtg gaatatgacg
     2941 gtatagatgt gtatgtgtgg aatggaaata tgaagccgta tgcagaggta aagcaaatag
     3001 aaaaataaaa gaaaacccaa tcgaatttcg ttcgattggg ttttgtatta ttctttgtct
     3061 tttaacaaat cgcgtatttc ggcaagaagt tcttcttggg taggacctgc aggagtgact
     3121 tcttcctcaa ctttcttttt gaatttatca tttagtttat taagtccctt aactacgcag
     3181 aaaattataa atgcaaggat aaggaaatta ataatagccg aaaggaaatt accgatattt
     3241 attgatgtac ccggaattat ccatttagac atatccataa ccgattcaac ggcacttgtg
     3301 tcaccgactg tacttgtagc ggctgttttg agtgcacctg ttataagaga tacaacgtat
     3361 gcaatacc
//
LOCUS       DAWACS010000121         3345 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3026_length_3345_cov_1.936474, whole genome shotgun sequence.
ACCESSION   DAWACS010000121 DAWACS010000000
VERSION     DAWACS010000121.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3026_length_3345_cov_1.936474"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    218..227
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3067..3076
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          982 a          562 c          740 g         1041 t
ORIGIN      
        1 gttttagtat attataagag agtaacaata tattcaaagg ggaatttttt tatgaaaaga
       61 aaatccggat taagtaaatt caacggtttt ttggcaatcg ttttggtaat ttgcgttgta
      121 gcaactgctt ttgtaattaa caaatatcct aagattgaag acgcttcggc agatgatagt
      181 tctaatacac aggatgtggc tgttattgat gaatttannn nnnnnnnacg tagttaatta
      241 ttataaagag tgctatgatt ataccaagac gcttactgct gaatataaaa ccgattcagg
      301 cgaaacacat agctactata agatgcttgg tacagagact cttgatgtta acaatcttct
      361 tgttgagggt aagtcaaacg acattatcaa caagcttgtt ccgggtattg taggcaatct
      421 tttccacggt ggcacaaacg gtctttcacc atcaggtaac agggacccta agggtgatac
      481 aagggatgac ggaaagatgg attgcacaaa gtcacttctc gagcctgatg atattcttgc
      541 agcaaatgtt aaggataacg gtgacggtac tattacaatg actattcagc cgaaagaaac
      601 tatcctttca atgcctggta aggacccaca aggtaggttc tttaactcac ttggtgatat
      661 ttcatcagtt gttaactcaa tctctgttct ttcattctca cagggtacag ttgatgataa
      721 ctttgtagtt gattacaagg gcggtacagg tgtatttgtt attgatacaa agactaacga
      781 aatcacaaag gctgattaca caatgttagt tcacattgat gttaagcatg caaatgttgc
      841 agttcttaaa gataagagtg cttcacttga cattacttat aaatgtcaat accctgcatc
      901 tgatgattat cttgcaggta aaaatatagg cttaacaaga gtaaaataaa actgtaataa
      961 ctttgattta aaggacttgt agaaatacaa gtcctttatt tttgtctatt tttattttaa
     1021 ggggttttca tattggggca aatgatatat aatataatca gcatttttat attctttttt
     1081 atatttaggg ggtaatagtg tgaaagtgct ttatgttatt cttatcttgc ttgctgcatt
     1141 tattgtgctt acgctgataa gagcgatttt ctttaaggca aagcctgtaa agagagaggt
     1201 ttttgaaaaa gaaaatgtta attcaaaacg tgttgaggag catttatgcg atgcaataag
     1261 gattaaaacg atatctcacg aaaacgaaga ggaaaccgat tgggcagaat ttgacagatt
     1321 tcgcgaattt ctgaaaaaat cattccctct catttatgaa aatttgagcg ttgaagatgt
     1381 gtcaaaagca agtttgcttt atttttggca gggaagtgac agttcccttg accctattgc
     1441 ttttttggct catcaagacg ttgtacccgt cagcgaggga acggaaaaag attgggtaca
     1501 ccctgctttt gacggtgtaa acgacggtga atttatctgg ggcagaggtg ctcttgatat
     1561 gaagaaccat cttatatgcc ttttggaaag tgttgaaaca ctgcttgaag agggatatca
     1621 gccaaaaaga agcgtgtatt tgtgcttggg tcataatgag gaaattgtat ccggttcaaa
     1681 aaacggcgca agagagcttg caaaaactct tgaaagccgt ggtgttcgcc ttgacagtgt
     1741 tgttgacgag ggcggagcaa tgcttccggc taaggttaag ggcatacttg acgctaattt
     1801 gacaggtatc ggtattgccg aaaagggata tgccgatttt aaaattacgg ttaaggctaa
     1861 gggaggtcat tcttctcagc ctcctaagca tacggctctc ggtgttctct caaaaaaggt
     1921 tgaagcgctt gaaaatcatc agtttaaggc gagaattttg ccgtttgtat ataatctttt
     1981 tacacagata ggcaaaagaa cgtcatatat tggcagagtt gtattttgca atctttggct
     2041 tttaaagcct gttttgcttg ctgttatgaa aaaaattccg cctgccgctt catttgtgcg
     2101 cacaacaacg gcggttacaa tgagttctgc ctcaccggcg gcaaatgttc ttccgcaaaa
     2161 agcaagcgtt acggtcaatt tcagaattat gcccggtgaa acaatagagg acgtaagacg
     2221 tcatattgaa aaatatatgg gcggtgaaaa tgttgaaata gaatttatca agggcaagga
     2281 gccgagtatt gtttcgccta ccgatacaag agcctttaaa acgctttcaa cgctttccgt
     2341 ttctcttgat gaaaaaaata tcgttgcgcc ttatctcgtt atgggcggta cagacgcata
     2401 taactatgaa aacgtttgtg aaaatattta ccgttttgct ccgtttacgg tcgatacaga
     2461 gcttttgctt accacacatt caacaaacga gcgtattcct gttgagcagc ttactcaggg
     2521 cgtggcattt ttcaagcgat atataagaat tatgacaaag gagtaatgat atgaatttca
     2581 aagaggattc ttttatggtg gctattgctc tttgcgttgt tttctttgtt gtcgcacaag
     2641 cacttttctt tcttgtgcgt gcagtaaaaa gggctaagga acttggaata gcaaatcaaa
     2701 caattaagaa tacaatcgtt tcgtcatcac ttttcactat tgcgcctgcc ataggcattg
     2761 tggcaactgt gcttactttg tcggcagggc ttggctatgt attgccgtgg ataagactga
     2821 cggtaatagg taatatttct tatgaggtca cggcagccac caatgcggtt gaggctttcg
     2881 gtcttgcggg cggaatatcc caaccgattg aaaacaaaga agtttttgca acggttgcgt
     2941 gggttatgac tctcggttca attatgccgc ttatccttgt tccgattttt ctcaaaaaag
     3001 tgcaaagtaa gatgaacaag gcggtatcta aaaacagcgc tctttcaagc gtgcttagtg
     3061 cggcggnnnn nnnnnnaaag cttgcaggcc ttgaaaagtt aaaatggctt gaaagctttg
     3121 caatgccgtt tgcaatgatt ggcgctatgg caattgtaat gattatggcg caaattttgc
     3181 ctgccgatgt tgcatttttg gaatggaggg gatagtatga acagcaaata tatcaataaa
     3241 actcatacat acggtaaaat ttggatgttg gttaccctct gcttgtttat tagtgttccg
     3301 gcgtttatat gtgttcatct cggtgtatct cccaaaatca atatg
//
LOCUS       DAWACS010000122         3341 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3029_length_3341_cov_2.552648, whole genome shotgun sequence.
ACCESSION   DAWACS010000122 DAWACS010000000
VERSION     DAWACS010000122.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3029_length_3341_cov_2.552648"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1038 a          645 c          729 g          929 t
ORIGIN      
        1 cagtaaacaa tcctgtaaga ccgcttgtag ctattcttgg cggaagtaag gtttcttcaa
       61 agatttcagt tattgaaaat cttctaaaga aagtagacaa gctaatcatc ggcggcggta
      121 tggcttacac attctttgca gcacaaggca agacaacagg tacatcactt cttgaacctg
      181 attacattga ctatgctaag aaaatgcttg acgaggcagg cgacaagcta cttcttcctg
      241 ttgatacagt agttgctaaa gaatttgcaa acgacgccga atcacaagtt gttgaaggta
      301 atattcctga cggctggatg ggtcttgaca tcggtcctaa gacaatcgaa ctttacaaag
      361 atgctcttaa agacgcaaag acagttgttt ggaacggtcc tatgggcgta tttgaaatgc
      421 caaacttcgc aaagggtaca aatacaatag ctcaggctct tgccgatctt gacgctacaa
      481 caatcatcgg tggcggtgac agtgttgctg cagtaaacca agcaggtctt ggcgataaga
      541 tgagccacat ctcaacaggc ggcggcgcat caatggaatt ccttgaaggt aaagaacttc
      601 ctggtatcgc agctttgaca gataaataat tattgtgaca gggcggtcat tgaccgcctc
      661 tccaccatat tttaaatatt ccgtagagtg actgtctgtc acttataatt aaactacaaa
      721 aggagaaatt acaatgggaa aatatataat tgccggtaac tggaagatga acaagcttcc
      781 gtcagaaaca tatgattaca tcaaagaagt aatcgacgca acaaagggtt cagaatgtga
      841 agtagtttgc tgtacaccgt tcgtatgcct accacaggct gttgaagccg ctaagggttc
      901 acacgttaaa atcggtgctg aaaacctaca ctttgaggac aagggtgctt tcacaggtga
      961 agtaagtgca gaaatgctta aagaccttgg tgttgactat gttatcatgg gtcacagcga
     1021 aagacgtgaa tacaacaacg aaactgatga aacagttaat ctaaaggcta aaaaggctct
     1081 tgaaaagggt cttattccta tagtttgctg cggtgaaagt cttgaacagc gtgaagccgg
     1141 tatcacaatg gactggattg ctattcaaat caagaaggca tttgccggaa tcagtgctga
     1201 cgacgctaag aaagttgtcg ttgcatacga gccaatctgg gctatcggta caggcaagac
     1261 tgctactgac gaccaagctg aagaagtttg cggcggtatc agagaattac ttgaaaacct
     1321 ttacgatact gaaacagcaa aggctattac tattcaatac ggcggcagca tgaatcctaa
     1381 gaactgtgca ggacttcttg ctaagccaaa cattgacggt ggtcttatcg gcggtgcatc
     1441 tctaaagagt gctgacttcg ctgttatcac aaaggcagct aaataatgat tttacaaaag
     1501 ggcaggcatt attgcctgtc ctatttttaa acataaagga gtaattattt atggcaaaga
     1561 aaccaatcgc gttaattatt atggacggtt acggcattaa ccaaaataca gagggtaatg
     1621 ctatcgtagc ggcaaagaaa cctcatcttg acaagctttt agccgaatat ccacattcac
     1681 agctttctgc aagcggtctt gatgtcggtc ttcctgacgg tcaaatgggt aacagtgaag
     1741 taggtcacac aaatatcggt gccggcagaa ttgtttatca aatgcttgtt aagatttcaa
     1801 aagacattaa cgacggtaaa atttatgaaa acaaggctct ttcagacgct atggacgccg
     1861 ctaaagctaa cggcaagtca cttcacctta tgggacttct ttcaaacggc ggtgtacaca
     1921 gccacaacga acacctttac ggtctgttaa agatggctaa gaaaaaaggt attgaaaacg
     1981 tatatgttca cacattcctt gacggtcgtg acgttcctcc tacatcaggt gccgaattta
     2041 tggctgaact tgaaaaagaa attaaagaaa tcggagtagg ctctgtcgct actattatgg
     2101 gcagatttta cgctatggac agagataacg cttgggacag agtagaaaag gcttacaaag
     2161 ctatggttga cggcgaaggc atacaggaaa cagacgctgt tacagccgtt aaaaactcat
     2221 acgcaaatga cgttacagac gaatttgtta ttccgactgt tgttgacaag aacggtatga
     2281 ttaaagaggg cgacagtgtt attttcttca acttccgtcc tgacagagca agacagatta
     2341 caagaacttt tgttgaccct gattttacag gctttgaaag aaaatatttc cctgtaaact
     2401 tcgtttgtat gactcaatat gacgaatcaa tgccaaatgt aactgtagca tatccgccgg
     2461 aaactcttga aatgacattc ggtgaataca tcagtaagaa aggtcttaca cagcttagaa
     2521 ttgccgaaac acagaagtat gcacacgtta cattcttctt taacggcggt gaagaaaagc
     2581 aatttgaagg tgaggaaaga atacttatca agtcacctga cgttgcaact ttcgatatga
     2641 aacctgaaat gagtgcttac gaagttactg acgcagttgt tgacgcaatc aattcggaca
     2701 agtttgatgt tatcattctt aactatgcta actgtgatat ggtcggtcac acaggtatta
     2761 tggaagcggc tgttaaggca gttgaagccg ttgacgagtg tgtaggcaga atggttgacg
     2821 ctattcttgc aaagggcgga caagctatca tcactgccga tcacggtaac gctgactgct
     2881 taatcgaccc tgttacaaaa gctccgttta cagctcatac aactaaccct gttgctatga
     2941 ttcttgtagg tgcaggtgat gttaaagtta agaacggcag actttgtgac ctttgcccga
     3001 ctatgcttga ccttatggga cttgaaaaac ctgaacaaat gacaggtgag agtcttattg
     3061 taaaataatc ggataataaa aaacgatgta ttccgtaaca ggtaatacat cgtttttatt
     3121 atgcaaactt ttcttttatt cttcttattt cattttccat catattaatt cggataagtg
     3181 ccatttcttt ttcgttctca acagttaaag ctttatcaag ttttcttgac aaatcaagat
     3241 gtccctctgc aataattttg atattacggt ttgtttcgtt ttcaagggta agactgattt
     3301 ctcttatttc atttttcatt ttttgaatat ccgttttcat t
//
LOCUS       DAWACS010000123         3314 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3065_length_3314_cov_2.421602, whole genome shotgun sequence.
ACCESSION   DAWACS010000123 DAWACS010000000
VERSION     DAWACS010000123.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3065_length_3314_cov_2.421602"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    235..244
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1917..1926
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1063 a          636 c          771 g          824 t
ORIGIN      
        1 atgtcaatcc atcagacgct gaaacatatg acgttaagcg tcacaaagct gcttccatat
       61 cagcactggg acttgattta agcacgggaa aaatcagttc taatagctct ttggcgaaag
      121 atgcaaatta tggtcctaag tggattgccg aatggaataa aaactattca atttccgaag
      181 ccggcgtgtc atggccgatt aataagcagc atttgtggag aaaaataacc gaagnnnnnn
      241 nnnncttgaa agcaactccg aacttaagtc tttgcttgaa aaggcgcatg cggcagcatc
      301 tgacggactg gcttctcagg ctgaggttga cgcggcatat aatactctta tattaaattc
      361 cggtaatcaa gatgtactga tgacggatac aatctatgtt tctccaaacg gagatgacag
      421 caacacgggc acaaaggata gtccgctgaa aacgctcgct gcggcacaaa gagtcgcacg
      481 aataaattcc gatggttcta aagacgttac ggttgtgctg cgcggaggcg aatataacat
      541 atcagatact ctgtgcttta catctgagga cggaggtaaa aacggacgta aggttacatg
      601 gaagggatat gaaagcgaaa tccccgtcat aagcggcgta actgaactta ccggctggag
      661 catttatgat gcagataaga acatatactg cgcttctgtt ccggagggct ttaatacccg
      721 tcaattatat attaacggcg aaaaggcgct gcgttcaagg aatatatcat actcaggcaa
      781 cacatataat catcttaaac gcacatgtga gcttggtttg aattccagaa ataatcgcga
      841 aatatatttt tatcgtgacg atattgcgga ctggaacaat tttgaaaatg tcgagttcca
      901 tctgattacc gcatggacgg ataatgttct gagatttgcg aaatatgata aggaaaataa
      961 ttttaaaccg acaaccgtta ataacggcgg cagtgaaggc gttgtcgacg cggcggcggt
     1021 aaagctgcgc gagcctgaaa gtacgcgctt gtttaaccgc gcgcaccctg atattacagg
     1081 aactacactc ggatataaat caagatcata ctattattta gaaaacgctt atgagtttat
     1141 tgacgaagat aatgagtggt atcttgacac aactgagaac aagatatatt gcaaggctcc
     1201 tgcgggttcg gatatgaatg atgtcagggt tacagcgcct gcgcttgaaa ctcttataaa
     1261 tatagacgga agtaaccaaa acagagtatg caacctgaat tttgaaaata ttgtttttga
     1321 aggttctact tggactcgac ccagcgagga gggacttgta ggaggtcagg catcgcaata
     1381 tatgctgact tcttctttag ccaataaagt aacagcatat cacactccga gcgcatttta
     1441 cgcacaatat gcggacagtc tcaatataac aggctgtaca ttcagaaaaa taggttcgac
     1501 tgcgattgac ctgtatttag gagttacaaa cagcaatatt ttcggtaatg aggtatatga
     1561 cactgctgga aacggtattt cggtagctaa attcttccag gatgaagaca ccgaatatca
     1621 tgaagcatat aacccgacgg ataaatcgga aatatgtgaa aatattaatg taataaacaa
     1681 taccgttcac gatatcggta ctgaatatga aggtgcggtt gcgattgcag caggatatcc
     1741 gaaaaatata gtagttgctc acaatgaggt ttacaatgcg ccgtattcag gtatttcggt
     1801 aggatttggc tggacaagta aagataatgc gatggacagc aatataattt ttgcgaacag
     1861 agttcataac gtaggacttg tttcgtgtga ttttggcgca atctatactt tatcaannnn
     1921 nnnnnnaagc cttggtatga ctatggctac acggcaatat attttgacga gcagacggaa
     1981 ggctatacta taaaagaaaa tctgacctat aatgtgggaa ataatctatg gggtaacgga
     2041 attaacttta acggttgcaa ggctaaaaac acggctacag ataattatct caacaccaac
     2101 cctaactcaa atgaaatagt gtccgctatc tcagctgagg caggaattgc agcagggaag
     2161 agctgtgcag aacttgaggc tgaggcagaa caatatattt acgatatttt acatccggaa
     2221 tataatacat acatccggat aacaccgact aaagtatcgg caacggcagc agacagcgga
     2281 agctcggcgg aatacgcaat tgacgggaaa gctgatacag tttataccct ttccggacag
     2341 actgcgaatt ctatggcaaa ccaatacctg cttattgaat ttgacggaga aactccaatt
     2401 gacagaattg taatcgaccg tcagtaccat gccggaggca taacacagta cgaccattgg
     2461 ccggactggt gccttgcagt gggatgtgag cttcagggtt cgtttgacgg tgaaaactgg
     2521 gagacgatag gagttatgaa ttcgtatccg gaaggtactg gtgatacaac ccaggatatt
     2581 tatgaacttg ataatccgca ggcgtataaa tatgtaagat atatacgaac gaagtataaa
     2641 tccggttcag attatggcgt atggttatgg tcaccgagca agggcggaga cggcggaaat
     2701 cgtttgaacg tgcaggatat cagcttttac agtaaaacta cgccacttcc tgtaaccggt
     2761 tcatatgata caaatacaac tacatataca tggacatttg ctaatgagcc tgaaggcact
     2821 gacactgcac agtcaagaaa ctatgatact gaggataaga ttgcgaatct tcgtcttgta
     2881 ctcagaagcg gcgactatgt aagtaaagac gaagggatcc acttcaatga taaaagcgtt
     2941 ggagacagtg gttctataca gaacagccgc tatatcctcg tgaagcctgc atatgacggg
     3001 acattatctg ttaccattgc gtttccagaa gcaaaaagca acgtaaagtg cagattatat
     3061 gcatatgatt atggcacgga gactgatttt gatagtattg atgaaacgct ggcaggaaag
     3121 ggcggaacaa tggtagccac acctgatatt actaacgcta atgctacaac gcggacatta
     3181 aaccttcagg gaggtcatac atatgcggtt tacacttatc aaagggcgtc aaatatctcc
     3241 gcgttttcct ataaggtaac ggaaggaagt acaccagaac cggatgtaac accaacccca
     3301 gagccggacg taac
//
LOCUS       DAWACS010000124         3308 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3074_length_3308_cov_2.217030, whole genome shotgun sequence.
ACCESSION   DAWACS010000124 DAWACS010000000
VERSION     DAWACS010000124.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3074_length_3308_cov_2.217030"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1003 a          629 c          630 g         1046 t
ORIGIN      
        1 gctttacttg aaaccgaaat gtattgacgt tgataaaatt aaggttgtca gaaacggtct
       61 taatctcgga atatatcgta aaaacagatt tgagccgtca tatgcgttgt gtttggcact
      121 taaaaaagaa gattttaaga ataccgttga ttttgaatgt gacagtgaag aacttaaaaa
      181 gtatcttatg ggtaatacag tcgaatgtga taaaaaaggt tggtgtgccg taactgtaaa
      241 cggttatccg ataggttggg gaaaagcgtc gaacggaata ctgaaaaatc attttcctaa
      301 atatttgaga ttgaaaagat agggagaaaa attatgaatt tgtttgaatc tgaacgtaag
      361 gttatggacg ttttgtggcg tgagggcagt ataaccgcag gtgaaattgc aaaaatactg
      421 aatattgata ttggatggaa cagaaacact acatatacag tgataaataa gtgcataaaa
      481 aagggttata tatcgcgtgg agaggataaa tttctttgta cccctgttat aacgaaagat
      541 gaagtaaaaa acgatgaatt ggaagaattg atgaaaaaat attttgataa ttcacccgta
      601 aaactgttta cttctgttgt aaatttggca gataaacagg aactaaagaa aatgaagaag
      661 attataaaaa ataccgctaa tctttagata ttagcggtat tttaattttt aatctttgat
      721 tgattgataa aattcattac gcaattcgtg gtcggtgtca aaaagaccgc gtatagcact
      781 tgtgcgtgtt ttagcaccgc gagccttgat accgcgagct gtcatacagc tatgctcacc
      841 ctcaacaaca acgattacgt cctcagtatc gcaaaccttt tgaataactt cggcaatatc
      901 cgagcagata cgttcttgaa gctgaagtct ttttgaaacc atatcgcata tgcgggcaat
      961 ctttgaaaga ccgataacct tgccgttcgg aatatatccg acgtggcaat gcatattgta
     1021 cataagtgca aggtgatgtt cacagtgact gaaaacttca atatctttaa cgattacaag
     1081 atcgtttgag tcaacctcaa agcacttgtt aaacatttca gcgatttcgt cattggtgta
     1141 tcgcataccc tcaaaaattt cgtcatacat acgtgcaacg cggtcgggag tatcctttaa
     1201 tccctcacgg tcggggtcgt cgccaagttc tgtaagaatt gtgcgtatag cctcttttat
     1261 cttttcgtga ttaaattctt ttttcattta aacacctctt tcttccggat cccagataac
     1321 cttgtgaagt tgaatttgaa atgttgcgtc acaaagcaca ggctctttaa gcattatatt
     1381 gacaagtttt tgtgcgtcat acattccgta aaccgcaccg agataaatat gcggcatttc
     1441 ggtataaact tgttttaact tcttgactat atcaatagtc aagttcatat cttcgtcaga
     1501 gcctataacg aatttgataa catcgcaagg ccgtagatta ataaaattat tccaaatcat
     1561 tttgtcggtc ataccgcttg acggaagttt gtagtcgata gtaaaaaaca aattatccga
     1621 cttcggcact ttgccgagaa aatcaactat atcaactgcg ccgttcgttt caatattaac
     1681 atcacagccg atttcggtca gttttgaaac aagctccgca ctgtcggtat gaacaagagg
     1741 ctcaccgcct gtaagagtga cacgcttgta agtcatatta acctttgaaa taatttcatc
     1801 aacagtcatt tcagtatatt cacaaggcgt ttcttcaccg aacagtgaat aaaccgtatc
     1861 gcagtacgaa cagcgtaggt tacagcctgc aaggcgtata aatgttgcac attgtcctgt
     1921 tcgtatgccc tcgccgtcaa tactgtcaaa aatttcgatt actttcattt tgattttcct
     1981 tataacagtt ttattttaac atcatctttg atgtagcttg caatgttgtc ctgactttcc
     2041 tgtacgtcaa cacgataaca gaacggtgca agctcatcgc aaatgtattt cgccaagttt
     2101 tcggcagtag gattgaacgg tacaacttca ttaataacct tgtggtcaaa cttgtcttga
     2161 attttgcgtt tgatgtgtgt aaaatcaagc accataccgt ttttgtcaag agtttcactt
     2221 tgaagatata cgtcaattat ccagttgtga ccatgaaaat tcgtgcattt tgagtcgtag
     2281 tcaaggtcta acttgtgtgc cgcacttatt tcaaatcttt tctttacttc gtacattttt
     2341 tcatctccat atattgttcg taaccgcgtc ttctgagcgt gcatgacgga cagtgtccgc
     2401 agccttcgcc gagtacgccg ttataacaag taagtgtttc gttgtaaata gtgtcaagta
     2461 cacccaaatc gtcagccatt ttccaagttt cagccttgtt cagccacata agcggtgtgt
     2521 ggataacaaa attgtaatcc atagcaagat tgattgtaac attaagagat ttaacgaaaa
     2581 tatcacgaca atccggataa ccagaaaaat ctgtttcgca tacgcctgtt acaaggtctg
     2641 ttatgccgtg agtttttgca tatatagcgg catagcttaa aaacaataag tttctgccct
     2701 caacaaatgt attcggcggt gcaccctccg gctttgtttc ctcaacggga atatcttcac
     2761 gagtaagtga atttgcggaa agctgattta taataggcgt cggaacgatt tcataaggaa
     2821 taccgacttt tttacaaatc ttgtctgcac attcaagctc aagcttgtga cgctgaccgt
     2881 agtcaaaacc tattgcagat acattttctt taccaaattt gtctattgcc cagaacagac
     2941 aagttgtact gtcctgaccg cccgataaaa caacaagtgc ttttctgttt ttcattttaa
     3001 tcctccattt attttaagct gtcaaacaat ctgttttcag cgattttttc atatttagta
     3061 cccggttgac cgtagtttgc aaacggataa atagatatgc cgccgcgcgg cgtgaaaata
     3121 cctttaactt caagatattt cggctccata agtttaacaa ggtctttcat tataatattg
     3181 atacagtcct cgtgaaaatc accttgattt ctgaaactga aaagatacag ctttaatgat
     3241 tttgattcaa ccattttcac gtttggaata tagttgatgg tgatttttgc aaaatccggc
     3301 tgacctgt
//
LOCUS       DAWACS010000125         3269 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3117_length_3269_cov_2.083385, whole genome shotgun sequence.
ACCESSION   DAWACS010000125 DAWACS010000000
VERSION     DAWACS010000125.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3117_length_3269_cov_2.083385"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2604..2613
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1133 a          524 c          727 g          875 t
ORIGIN      
        1 gaattgtccg ccgtgcatat gacctgcaag aataaggttg aaaccgcggt attttaatct
       61 gtcgaataaa ttcgcacggt gaaaaagcag tatgtcatat ccgttgtaac gtggcagttt
      121 cgcaagtgaa tcttcgatat tttcggcgat tttgacactg tttaaagcaa acggatcgtc
      181 aattcccgaa agtgaaattt cactttcgtt acgatttaaa atgacacgct cgtcatgcaa
      241 tgttttaacg ccgacttcgg taagttcccg ctcaaattca tcgtagtcac ttcgccataa
      301 atcgtgattg cccgttaccg cataaacggg tgcaatgtcg ataagatgtt tgcatagacg
      361 tactccgggt gtgtatgagc ctgtgtcgtg aacaaggtcg cctgtcgaaa ctataatgtt
      421 gggcgattcg tgttcgattt cttcaatcag tcccggtacg ctgtctgcgt ggtaatctga
      481 aatttgtaaa attttatatc cgtcaaatcc ttcggggatt tttgatgacg atatatcgta
      541 atgtgtaact tcaattctgt tgtccaaacc tttgacggca aataatattc cgcccgtaag
      601 cgctgctgtt tttaaaagct ttttcacaat aatctgtcct ttcagatatt tatgggttta
      661 ttataccact attttttaat aaaaaccaca tattttattg aaaaaattaa aataagatga
      721 tataataaat gtatatgtat ttaggaggaa tttaacgtgg ttaagcatat agtaatgtgg
      781 agaatggcag agagtgaaga taaagagcag agagcaaagg atataaagga aaatcttgag
      841 gctttaaagg gtaaaataga tgttttggta aacattgaag tcggacttaa tttcaacgat
      901 acagacgccg cgtcagatgt agtacttgta tcggagtttg aaacaaaaga ggatttaaac
      961 acttaccaaa atcaccctga acacaaagct gtcggtgcaa cgtatgtgcg tccgtatgta
     1021 gtggaaagac gtgtttgcga ttatgaattt taagttgtaa ttacaattta aatgtgatat
     1081 aattaacttt acttttgaag tataggagaa atgtaaatgg caaagcttat aactaaggag
     1141 cagatggcgg agcaagaaag ttatataatg aaactcaaag aagaaaatat gcgttatgct
     1201 gctcaaaacg gacacgcaaa attagcgctt accgaaacat acggttgtca gcagaatgaa
     1261 aacgacaccg aacgtataag aggtatgcta agacaggcag ggtttgactt tacggacgac
     1321 agcaacaaag ctgatgttgt tatatataat acttgtgcgg tgcgtgaaaa tgcggagcaa
     1381 aaggttttcg gcagactcgg tattttaaag cacatcaaag aagaaagaaa agatatggtt
     1441 atcggtgtat gcggttgtat ggtacaacag gagcatatta ccgagaaaat caaaaaagta
     1501 cacgaacacg ttgaccttgt tttcggaaca cacgcacttt acaaaatgcc ggagctttta
     1561 tacagagcaa ttcacgaaaa gaaaacagtt gtcgatattg acagcagtga cggtgcaatc
     1621 gcagaggata ttcctattat gcgtgacgat gataaaaaag catgggtatc tgtaatgtac
     1681 ggctgcaaca atttctgctc atactgtatt gtaccgtatg tacgcggacg tgaaagaagc
     1741 cgtacacctg aggcggttat agccgaagtt aaggaacttg tggcaagagg ttgcagtgaa
     1801 attgcacttt taggacagaa tgtaaattca tacggcaaag accttgacac agatgttgac
     1861 tttgcagacc ttattcgtat ggtaaatgat attgagggcg ttgaacgtat cagatttatg
     1921 acatcacacc caaaggattt gagcgataaa cttattgatg cgattgcgga atgtgacaag
     1981 gtttgtaaac aacttcatct tcctgttcaa gcaggttcaa acaagatttt gaaagaaatg
     2041 aacagaaagt atacaaaaga agattatctt gcaaagattg aaaaggttaa aaagagaata
     2101 ccgaacattt ctctttcaac cgatattata gtcggttttc caacggaaac aaaggaagat
     2161 tttgaggaaa cacttgacgt acttcgtaag gttgagtatg ataacatatt ctcatttata
     2221 tattcaagac gtgagggaac tcctgcggca aagcttgatt ttgtacttac agacgaagaa
     2281 atacacgaga atttcaacaa gcttttggaa gtacagaatg aaatttcaaa acgcaaaaat
     2341 taggcgtatg taggacgggt tgaaaaggtg cttgttgacg gtgtaagtaa gaatgacgaa
     2401 acaacacttt caggacgttg tgacagttca aagatagtaa actttaaggg cgataaatcg
     2461 cttataggaa aatatataga cgtaagaatt accgaggcac acacatggag ccttaacggt
     2521 gagattgcag aataagaaag gaatttaata atatgaacga aataatcgaa aagacaagag
     2581 aacttggaga acttatccaa aatnnnnnnn nnngaagaaa tgaaaaatgc aaagaatgcc
     2641 gagattttac aagaaaatga tgacgaggca caggcacttt taaaggaatt caacttgcag
     2701 agaatgaacc ttgcacgcga tatgcaaaac aacaagattt cgcgtgaaga ggctatacag
     2761 aagaataacg aagcatttga agaaatgctt gaaaaatcgg aatcaataaa gaagtacatt
     2821 gatgcaaaga aagaatttga cgcaatggtt aatcaagtta atcaaatgct gaatttctac
     2881 atcacaggac aagatccaaa ctgtacgcat gattgcagca cttgcggcgg ctgtcactaa
     2941 tttatacaca aatctaaaga caaggagata aagcggaatg gcaaaagagc agatgacgcc
     3001 gatgatgact caatatctga aaacaaagga ggaatacgag gactgtatat tattttacag
     3061 attgggtgat ttctatgaaa tgttttttga tgatgccata aaagcttcaa aggagcttga
     3121 aataacgctt acaggtaaag actgcggact ttcggaacgc gcaccaatgt gtggcgttcc
     3181 ttttcacagt gcgtcggttt atattgcaaa gctgattgaa aaggggtata aggttgctat
     3241 atgcgaacag gttgaggacc ctaaaaccg
//
LOCUS       DAWACS010000126         3254 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3142_length_3254_cov_2.277899, whole genome shotgun sequence.
ACCESSION   DAWACS010000126 DAWACS010000000
VERSION     DAWACS010000126.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3142_length_3254_cov_2.277899"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          917 a          642 c          496 g         1199 t
ORIGIN      
        1 ccgtataacc gccgttttct gacggagaag ttgaataagc agtagcagtc attgtgattt
       61 tcttcttata tttatatccg tttacagtac cgccgttatc gcttactgtc gactttgtcg
      121 caaaggtgtc cttaacggtt gtattcttag gtgcaggtgt aggttccatt gtaccttttg
      181 caataacttt attacgcatt tcagttgtaa cttgttcgtc tatcacctct cttgtcgcct
      241 cttgaccatc ttggtatgta accttgtaag tgattgtttt cacaccgtct tgtccttcgt
      301 caacaacctt ttcctgacct ttaatcaaag ttgaatcatc aacatattca acacccttag
      361 gaattacttc cgtttctgtt tcatcggtat ggcttaccga tacaagctcg attgtatcac
      421 tgctcgcgac attatcgccg ttagaagaaa tttggtcata ttcacccgga atatatcctg
      481 cctcaacaag tgcgtcctta acgtccgcct ttgttactgt tacaacttct tccgattcac
      541 cgactttaat tgtaacacgc ttaccacgct tgataacaat attttcatta tcctcaacag
      601 gcttttctgt tgaaacatta atcttatcgg tatccgaaac attcacgcca tgttcttcaa
      661 gagcaccctc atcgctttca cttcttgtct ttacagtttt tgtttcattt gttcccgcaa
      721 attcgtttat ttccgtaatt gtgatttcct tcgccgatgc gctgtataca ctaccgaata
      781 ttaccgtcat cattgccaca agcgtaataa ttgcaatcgt acatcttctg tgctttgctt
      841 tcaattttgc ttttttcttt tcttcgcgtt tttcacgcaa attcattatc atttcttgtt
      901 ttgtcatctc ttgtgtagtc ataaaagtta aacccctttc attacttgtc ccatagtata
      961 ctacaaaatt gttacattgt caagcttttt attacatttt tattacaaaa taatttcaaa
     1021 tattgcaata tgaataaata tattactcga cttttcccat atattactat ttatatgcac
     1081 gatatttaca tattattaca tttatttttg attaatgtta caaaaataaa gcaggtatat
     1141 actttttcat agtcggaaat aatggaaata aaacaaattg aaatgattgg agattagtaa
     1201 tgaaagaatt gacaaagaaa gtagttattt taaataattt ttcatcaccg tatgtgagcc
     1261 aagcaataat aattctcaaa gactataatc caaaacttga aagcagagca atagcggacg
     1321 cggaaacaat agtcagtcgc tacatcgaac gaatacaaaa aaacggacaa ccaacaaagg
     1381 ctgtccgctc gaaatctaaa atattaaaaa tattgatttg cttaattttg attgcgtcaa
     1441 tatgttttgc aatcaaatat ctttcttaaa ttatttgaaa ttgctgtcca tatagtcaat
     1501 atattcagta tatttttgag ctgttgatgt catcgaagaa tatgagaaat cataatatac
     1561 cgcaattccg tttttcaata cgcattttct gtggtaaaca gtatcatctg ttgtcacagt
     1621 gactgcatac caatcgtcac cggcacgaga atatgaaact tcgccgcccg tttgacgtgc
     1681 atagtcacgc ataaggtcag acggagcacc gctctttccc tcttgcgaaa cagtcatagt
     1741 tgcaccgcca agcgcgtcag tcaagttaag tttttgattt cctgttgtcg gatttgaatt
     1801 aaaagtagac ggataaacgc acgtcacacc gcttattgtc attttcgaat aaccgttttt
     1861 gcttgctgaa tttgacggcg atgatgttga tgatacgttg tttgctgtat ctgtcggcgt
     1921 attcgttgca cttgcaggcg aatttgttga agtaggagca gtgcctaatc cgttatcgtt
     1981 cggagccatc atattatagc cgataatcac gcttactatt gcgataagca gtgctacgat
     2041 tatgaaaatc ataacacttc ccttactttt tctacgtttt ttattacggg cttgttttgc
     2101 ctctttttcc tgtaaacgac gtttacgatt aagacgttca gccttcagtt cctcgatttc
     2161 tttgcgtttt gtagtttggc tgttctggtt gttttttctt actgattgac cgcatctgtc
     2221 acaaaaacgc gttccctctt tcagttgtct gccacaattt ttgcagtaca taaaacccct
     2281 ccgaaattac ttttttgcta tctaaactat tatactatat attgtatata aatgcaagta
     2341 attttcgtaa ttttgttaca tatagattac acattaaacg caaaacacga aatatttttg
     2401 aaatatttgt tatttaaacg caaaattcca tattttatta taagaaatta tcatagcatt
     2461 tacatttatc gacaaattct tccctccatg atataataac attctgaaat ggttgcaaac
     2521 catgctgaca ccaccttaaa gggggtgtgc atatgagtga agctattgtt tatttaactt
     2581 tggttttact gctttgtata gtgataataa aaaaatagtt acctatcgac caaataggta
     2641 actatttcgt agaaagtatt tcttcctaca acatatcaac tgcagcatgg tttgtttacc
     2701 atttctttta ttatattatc acacaacact aaatttgtca agcaaaaaga acggtcatac
     2761 gaccgttctt ttattacttt ataaattatt cagttgcaga tgttgttgca tcactttctg
     2821 ttaccttgat attatcaagc attgcacaat ggctgttgtt tatataacca aaaatatcaa
     2881 tttgtgttgc ttttaagttt tctgttgttg catcaacaaa agccacatct gttgcttttg
     2941 ctatttctgc accatctgct ttatttgtga tagtatagct tactttcttt gttgattgat
     3001 taacaactac attgacatgc atccatttat ttgtatagtc acctatagct ttagagctgt
     3061 caccttgaac taatgcatta gttttattcg atcctaaatt aaatatagca cctgttgaat
     3121 tgtaagtaac ttttgtacta ccaccagtag ttcctcttgt tgttgcatca ccaatgctca
     3181 atattgctct accaccgttt gtattataat aatcaaaatc aaatgttact atatcttttg
     3241 ttactgtatc tggt
//
LOCUS       DAWACS010000127         3254 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3141_length_3254_cov_2.568928, whole genome shotgun sequence.
ACCESSION   DAWACS010000127 DAWACS010000000
VERSION     DAWACS010000127.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3141_length_3254_cov_2.568928"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          841 a          741 c          489 g         1183 t
ORIGIN      
        1 gtcctcttgt gtatgtgttt tccgtcatac tctgcacatt tttttcgccg tttaaagctc
       61 tcttatacat atctattatt ttatcacatt gagcgaattt ttcaacagta aatatcaatc
      121 tgatactgtt tattttaagt ttttttaaat ccgcgatttt atccgccata aatataggct
      181 ttgaatttaa aagttttgct atacagcctt ttgaacagac aatcggaaat tcctctcttt
      241 ttctgtcttt cagcttgtat atcattttgc ccttttggca ttttcccata gcctttatcg
      301 ggcagttttt catcaacata agcggaatat gtccgtagcc tataacttca agattagcgt
      361 ttgtattttc acataaatcg gcaatttcat gtaaattaag ttcaggagat aatgtaaccg
      421 ttttcaaatc gccgtaatgc tgtgccgttt gcgaattaaa aacattcaat ctgaaatcgc
      481 cgtatatctc tttatcttta tacttgtaaa ttgccgccgg tgacgaaaca agtacgccgt
      541 ccgtctttat attctcttca caaaatatat ccgccgattt tgatattatt tcaacatcgt
      601 caggcacatc ttttagtgtc cccaccaaat cattgggggc atatattctt tttatgttat
      661 ttttaactgc ggctttgaac tgttcttcat cactaacttc acaagtgagt attatattcg
      721 tgtttttacg gttgctatgc tcaatttcaa agtcaagttc tctgcctttt tcacgcattt
      781 gtctttgatt tataaggctt tcgcaacatt cacgtcttac gctgtttatt tccttaatcg
      841 gaattgtaat tccgtcgtct atcgtaatat ttatatcgtc acattcaaac ggtgtacttc
      901 caagttttaa aagctggtcg cgaagtcttt tttcgtccat aggtttatgg agtgcttttt
      961 cggattttag tgtcccttgt gcatacgcgt aatgtccgtc attatcgtac attgtaagtt
     1021 caagcggagc atcaatattt agtgtcccca taatacctgt gttgatttta cggatatttg
     1081 cgtcctctgc ggcacgtttt ttcgcctcat cggtaaattt attttccgca ctgttactcg
     1141 gagtatcgtg gctcatcata tttttaccga gattgccttt aaaatatccg tctgtaaaac
     1201 ctgttcttga aaatgcgtca agcagttcct gcatatcttt ttttgacacg tttccgccct
     1261 tgtcaagata ctttcggtac acgcccacaa cagcggatac atattccgct ctttttaagc
     1321 gtccctcaat tttcagtgat gttacgccta tacgttttag tgtccccaag tcattcacaa
     1381 gtgccatatc tttaggactt aaaacatatc cgcttttaac cgttttgccg ttttcaagca
     1441 aatcatacgg caatcgacac ggttgagcgc aacgacctct gttaccgctc ctgccgccta
     1501 aaatactcga cataaggcac tgtccggagt aacacataca aattgcaccg tgaacaaaaa
     1561 cttcaatttc cgcttttgca tttttgcaaa tatgctctat ctctttttcc gacaattcac
     1621 gcgccaaaac cactcttgaa aatcccatat cctcaagata ttttacaccc tcaagacttg
     1681 taacggtcat ctgcgtgctt gcgtgtaaag tcatatccgg aacagtcttt ttgataatct
     1741 ccacagcgcc catatcctga acaataagtg cgtcaacgcc gattttatta agttgcttca
     1801 aatattcttt aaggctttca atttcctttt ccttaacaag cgtatttacc gtaacgtgaa
     1861 cgtcaacacc gtataaatgg cagtaatcca cccacgtttt catttcttcg attgtaaagt
     1921 tttcggcact gtaacgtgca ccgaactgcg gaccgccgag atataccgca tccgctcccg
     1981 attgtaccgc cgccgtaagt gcctgtctgc tgccggcagg tgctaaaagt tccatactca
     2041 tttaaccttc ttcttgccgt ctgatacaac tgtattttct tcgtatgctt ttagtttttc
     2101 ttttaaactt ttaatagttt cttcaagagt tttattgttt ttatttgctg tttcaaggtc
     2161 tttttttgtt gatgttaatt cggcattcaa ttctgtttca tcaatcgtaa cctgtccgct
     2221 ttttgcgtca tcaagttctc tttttagcga gtcgttctct ttttttaaat tatcgatttt
     2281 tgaatttaat ttttctattt caacctttac cgaatcgtcc gattcaagtg ctttgaaata
     2341 ttcgtcacaa atattaagtg ccgccaaaac aataggacgt tccccaagaa cattttgacc
     2401 gccattcaaa acgtttttaa ccttttcatt tatatgttcg cccaatcttt cgatatactc
     2461 tctgctttcc tcagcaacaa ctgtatattg acgtgaatta atggttattg aaactctgtt
     2521 caccgccatg acttcatctc cgtttcttaa ttttttcgca taaaatacct aatatactct
     2581 tattatacaa taaaaccaat taaaaaaaca gagttttttt gacttttttc aaaatatttt
     2641 ataaaaaaag aacggtcacg ccgttcttat aattctatcg ttaattttct gcccgttttt
     2701 tcatatattc tgtatttaat acctaccatg tcaaacattt tctttgacgc aatagtgtta
     2761 gcctcatcgg cgtatttgtc ggacatatac acaacttcct tgataccgct ttgaataatc
     2821 gccttcgcac attcgttgca agggaacagc gttacataca gcttagcgcc ctcaagcgag
     2881 cctgaacttc tgtttaaaat cgcattaagc tctgcatggc aaacgtacat atatttagta
     2941 tttacaggat tttcaccgtt tctttcccaa ggcatatcat catcgcaaca ccctgtcggc
     3001 ataccgttat agccaagcga caatatttta ttttcatgat ttacaataca cgcaccaacc
     3061 tgtgtatgcg agtccttgct cctctgtgcc gacagtaacg caatccccat aaaatattcg
     3121 tcccacgata tataatcttg tcttttcatt ttcaatcctc caacaaaaaa tctttcgtca
     3181 tatgataatt ttaacattcc taaaatcatt agtcaagaaa aaaagccgtc cgattttttc
     3241 aatcgaacga cttt
//
LOCUS       DAWACS010000128         3253 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3145_length_3253_cov_2.201063, whole genome shotgun sequence.
ACCESSION   DAWACS010000128 DAWACS010000000
VERSION     DAWACS010000128.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3145_length_3253_cov_2.201063"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          796 a          773 c          532 g         1152 t
ORIGIN      
        1 ctcttgggag cgaactttga accttcagca ggttgcaatt cgtttaagtt catttgctac
       61 acctccttac taattagttt tcttcaactg ttacaaggtg agataccttg ttaatcattc
      121 ctctgatttg aggtgtgtca ttgtgttcaa caacacttct tattttcttt agtccaagag
      181 cttcaacagt agcgatttgg tctttctttc tgccgattgt actcttaact aatgtaattt
      241 ttagcttagc catattactg tgtgcctcct tattaacctc tgatttggtc tacagtcttg
      301 cctcttagct tagcaacttc ttcaggctgc tttaactctg acaatccctg tatagtagca
      361 ctaactacgt ttctcttgtt atttgatctc aagctctttg ctctgatgtc tttaacacct
      421 gcaagttcaa gaaccgcacg agcagcacca cctgcaagca caccagtacc ttgtgcagcc
      481 ggcttaagaa gaaccttacc tgcacctgaa ataccaacaa cttcatgagt aattgttgaa
      541 cctacaagag gaacagaaat caaattcttc tttgcgcttt caataccttt tctgattgcg
      601 tcaggtattt cagcagactt accttgaccg aagccaacgt gaccatttct gtcaccaaca
      661 acgaccaaag cgctgaatct cattgttcta ccgcctttaa cagtcttagc aacacggttg
      721 attgctacga cattttcctc aagctctaaa gcttctacgt ctattctttc agccacttat
      781 ctcttcctcc ttctattaga attccaagcc gccttctctt gcgccttctg caagagcagc
      841 aactctgccg tgatatacat atccgcctct gtcgaatact actgctttaa tgcctgctgc
      901 aagagccttt tcagccactt tctgaccgat tgcctttgca ccttcaacgt ttccgccgta
      961 gccttcaaat tctttgtcca tgcttgatgc tgaaacaagt gtaacaccct ttacatcatc
     1021 aataacctga gcatatatat gttttgcact tctgaatacg tttagacgag gtctctccgc
     1081 tgtgccggag atgttctttc taactcttgc gtggcgtcta actctagccg cttttgtatc
     1141 tatcttctta atcatttaag aacactcctt tcaattattt cttcttagct cctgttttac
     1201 cttctttacg tctgatgtgt tcatcaacgt acttaatacc cttacccttg tatggttcag
     1261 gctgtctgta ctctctaatc ttcgctgctg ttgcaccaac gtcttctttg ttggcacctt
     1321 taacgatgat tttgtttgga gcaggaactt cgattgttac gccttccttt ggttcataca
     1381 aaacaggatg tgagtaacca agactcaagt taagagtctt gccttgcatt gcagctctgt
     1441 aaccaacacc gttgatttca agctcttttg taaagccttc tgtaacaccg ataaccatat
     1501 tgtttacaag tgttcttgta agaccgtgca atgacttgtg catcttatct tctgaaggac
     1561 gctctactgt gataactcca tcgttaagct taacgatcat atcaggatga agttgtcttg
     1621 aaagtgtgcc gttcttacct tttactgtga tttcgtttgt atcactgatc tttacctcaa
     1681 ctccggcagg aacagtgata ggtaacttac ctattcttga catagggatt tttcctcctt
     1741 aacttaaatt tgctctgttt tacacacagc cgtgtgtttc cacaggtttt cagttatttt
     1801 tttattaata ttaattacca gatgtaagca agtacttcgc cgccaacgtg ctgctttctt
     1861 gcttccttat ctgtcataat accctttgat gttgaaacga ttgcgatacc aagaccctta
     1921 agaactcttg gaagttcgtc aacgcttgcg tagaatctaa gaccaggctt agaaactctc
     1981 ttaagaccgc ttataactct ttccttgtta ggtccgtatt tcaaagtaat tctgataacg
     2041 ccttgtttac cgtcttcgat tacttgaaaa tcttttatat aaccctcatc attaaggttt
     2101 catgctttga tgagcttgca tttctgattc ttgtaagcaa atctgcgatt ggatcagtta
     2161 tttgcattta ttttacctcc ttacgctctc acaaaccgca agcggtttgc cttatgctac
     2221 ggtatttgat gtcttgcacc aaatacctgc ttcagctata acttgctgtg caagtcactt
     2281 tacctctttc ggatttttgt aagtccgaat tttatcatat atctaaaaat caattcgttt
     2341 cttataagaa acgctcgatt ttaccagctt gcttttctta cgccagggat ttgacctttg
     2401 taagccaatt ctctaaagca tatacggcaa ataccaaact ttctaaggta agcatgcggt
     2461 ctgccgcaaa tcttacatct tgtataagct tgtgttgaat acttaggttg tttctgttgt
     2521 tttaaaacca ttgctttcct tgccatgtta aatcctcctt tttaactatg aacggaacgg
     2581 agcgcccatt aactttagaa gttcacgagc ttcttcgtct gtctgtgcag ttgtgacaat
     2641 gttgatgtcc atacctctga ttttgtcaat cttatcatag tcaatttcag ggaatataag
     2701 ctgttcctta ataccaacag aatagttacc tctaccgtcg aaaccgttag ggttgatacc
     2761 tctaaagtct cttacacgtg gaagagcaac attgaaaaat ctgtctaaga attcatacat
     2821 cttgtcagct ctaagtgtta ctttacagcc gatattcatt ccttctctta acttgaagtt
     2881 cgcaactgac ttcttagcct ttgttataat aggcttttga cctgttatag cagccaaatc
     2941 tccgcaagca gcctcgattg ccttagggtt gtctttagct tcacccatac cgatatttat
     3001 aacgatcttg tcgagcttag ggatttgcat tgtactttta tagctgaact tctccataag
     3061 agccggagca acttctttat tatatttatc ttgcattcta ctcattgtta gaatcttcct
     3121 cctctcgaaa attaatcgtt aagttctgca ttacacttct tgcagtatct catctttgaa
     3181 ccgtcatcaa gaaccttaac acctgttctt gcagctttac cgcactttga gcaaactctc
     3241 ataacgtttg atg
//
LOCUS       DAWACS010000129         3225 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3185_length_3225_cov_1.921451, whole genome shotgun sequence.
ACCESSION   DAWACS010000129 DAWACS010000000
VERSION     DAWACS010000129.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3185_length_3225_cov_1.921451"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    702..711
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2733..2742
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          907 a          790 c          482 g         1026 t
ORIGIN      
        1 gctataaact ttcatgcctt ttacactaaa tgcgccttcg ccaccatact cactatgttt
       61 tatcacccta taataatcat cggcaaaatc gacagtgtct ttcttattcg actgtaaaag
      121 cacagcattc aatttataaa cagtctcacc cgtttccgca ctgtatatat cagaacactt
      181 gttatatcct gctgaaacat tgcttttatt attatgaata taataatacc atttaccgct
      241 tttttcaaag aatataaatg acctctcatt gtccaaagta ttatcctccg gaccatcata
      301 tgtaaatatg gttttaccat ctttttttat ccattgctta tcctcaccgc taaaaagtga
      361 taaatcaccg ttaattttta cttcatacac attgcttgcg tagtcactca catcaagatt
      421 aaacaacttt agcattgtta ttgccgactg ctccattgta taatttgatt ttggcgagaa
      481 attgttatca cctactcctc gcattattcc gagagaatcc attttacgca cagattcctt
      541 tgcccaatcc gaaactgtgt cataatcaga aaatacagta tcaaataaag ctatgtcctc
      601 ttttacgccc ataaattccg ctgtattcgt cagtattact gccgcctcct cacgcgtaat
      661 atcgtcattc ggtgcaaatt ccgtttcaga acgtccgttt annnnnnnnn ncttctttat
      721 catcggtatc agcaaacgaa ctcttaacat tattctcact aatcattgat ttttgttcaa
      781 gcattttata tgccaaatga caaaaatcaa tacgcgatat attagcggtt aaatctctgt
      841 ccgcaaagaa attcggcaat aattccgctt catctgcact tatcaaactt tcctccgccc
      901 attgtgaatg actttcgctt acttccgcag aagcacctag tcccgacgct aaaatcattg
      961 acacacataa cgcacctaaa acaaacttct ttttcatttt ttctccccct ttaatattaa
     1021 aaattatttt tcatcttctc ttctgttata caaaaacatc gctaccgaca cggagcatat
     1081 tattacatag ccgataatac tgaatttatc gggaatatcg ccgaatgcta taaaccccat
     1141 aagtgccgca aatattattt gcgtataatc gaacactgat atttctcttg ccggtgcata
     1201 tgaatatgcg gcagtaatcg aaaattgtcc gcctgccgcc gctattccgg cacatataag
     1261 tattacaagc tgtgctatcg tcataggtga atagtcaact atcaaatacg gcaatgtcac
     1321 aacacacgaa aatgccgaga aaaagaatac tataaacgga cctctttcgc ctttctgact
     1381 taattttctg accatagtgt acgccacacc tgctccgaat cctccgagca ttcccacaaa
     1441 gcttggcaag cttgccgata tcgactgtgg ctttattata aacaacgcac caataaatgc
     1501 cacgattacg gctaaagcct ggaacggctt taccttttct ttaagcacaa aatacgagca
     1561 tataaccgca aaaaacggtg acagcttatt aagcattgac gcgtccgaca agacaaggtg
     1621 gtctatcgca tagaagttac acaaaattcc cattgtaccg aatattgacc taagcaataa
     1681 ttcaggcaaa ttctttttat tgaacttaaa ttttccgtct gttcttatca gtacaacaaa
     1741 tgatataata agtgctatga aatttctgaa aaaacttttt tgtattgacg gcaaatctcc
     1801 tgccattctt acgaacatat tcatcaatgc aaaacaaaac gccgatgaaa ttatacacat
     1861 tattcctttt actttattac tgctcatttt tacccctacc gttattaatt gctaccattt
     1921 ccgctgtttt tattccgtca actgcggcgg acataatacc gcctgcatat cccgcaccct
     1981 caccgcaagg atacagacct tttatgtttg actgcattgt tttcttgtca cgaattatcc
     2041 ttacaggtga tgatgacctc gtttcaggac ctgttataac cgcaccgctg tcggcaaatc
     2101 cgtgaagttt gttatccata agcactattc cctcacgcat tgaatcggtt atgaatttag
     2161 ggaacacttc atcaagattt acccacgtca cattaggctt atatgtcggc tcgaccgtat
     2221 attcactgtt actttccgtt ccgagaaagt caccgactct ttgacacggt gcattataat
     2281 tcttgccgcc gacttcaaat gctctctgct caatttcacg ctgaaaatac attcccgcaa
     2341 gcggatcgtc agttttaaaa tcgctcggtg ttacatttac aagcaatgcg ctgtttgcgt
     2401 tttttccgtc acgtgcaaaa tagctcatac cgtttgtcac aacaccgccg tcctccgatg
     2461 cagacgcaac aactctgccg cccggacaca tacagaatgt atacacacct ctgccgcttt
     2521 caagatgtac cgccatttta taatccgccg ccggaagttt tttatacgct ttaccgtact
     2581 ggcttttatt tatcatttct tgagaatgtt ctattctcgc accgactgaa aaactctttt
     2641 gctccattgc tatattgcga ttttggagca taagaaaagt atctcttgca ctgtgaccga
     2701 ttgcaagtat caccgcattg ctttttaatt cannnnnnnn nngtgaccga ttgcaagcac
     2761 aatattattt gtttcaatca catattcacc gctgtcactt tcaacaaccg caccgcatat
     2821 ttcaccgttg tcggttttta cattgacaag tttattggaa aatctgactt caccgccgag
     2881 tttacgaatt tcacttctga ttgacgcaac cattttagaa agattatcgg ttccgacgtg
     2941 tggttttgaa taatataata tttctttcgg tgcaccgtgc ttatgaaatt cttcaagcac
     3001 ctttcttata cgaaaatctt ttatacccgt tgtaagtttt ccgtcagaaa aagttcccgc
     3061 accgccttcg ccgaactgta catttgattc gggatttaaa aagcctgcct cccaaaatgc
     3121 ctcaacgtct ttcattcttt cttctacgca ctttccgcgt tcaagaagta ttaccttata
     3181 cccgttttgt gccaaagtaa gtccgcacat aagtcccgca ggtcc
//
LOCUS       DAWACS010000130         3205 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3217_length_3205_cov_1.644127, whole genome shotgun sequence.
ACCESSION   DAWACS010000130 DAWACS010000000
VERSION     DAWACS010000130.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3217_length_3205_cov_1.644127"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2195..2204
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          923 a          707 c          508 g         1057 t
ORIGIN      
        1 cgagggttat gagtgcaagt atcattgccg ccgactcaaa atacaaattc atcatatatg
       61 aatgtgcaaa ttccttgtca ccgtgtccga gtccgtatcc tatgcaatat attgcaatga
      121 ttccatacgc gaatgccgcg cccgaaccta tcgctatcag cgaatccata ttcggtgcac
      181 cgtgaaacaa cgtcttaaaa cctgttatat agtattttct gttgacatac attatcggca
      241 acgtcaacag catttgcgta agtgcaaatg ttattccgtt ttctgcaccg tgaaacacgc
      301 tcaaaaacgg ccaaccccac atatgtccca ttgaaatgta cattagcggc acaaggcaaa
      361 caagcgacac aataagtctg aacttcatat ttgaaatttc attgtcgatt ttgttgtctt
      421 tcttttcttg ttttttcccc gcaacagacg caccgtatcc ccccgaaaca actgcgttta
      481 taatatcgtc aacgcttaca gccgtttcat caaaatcaac gtgcatatta ttctgcaaca
      541 aatttacgtt tacagatttc acaccgttaa gttttttgac gcttttttca acgtgtgcgc
      601 tgcacgcact gcacgtcatt cccgttacat taaatttttc agtcacatta tcaccgtccc
      661 gaatattttg attgaattta tttattgttt atgatattat tatatcgtaa attatttatt
      721 tttcaagtat gcactttttt gatagatact atcatttatg atagtatgga ttggaggtaa
      781 tacatatgaa ccacgattat atatgccctg ttgaggcaac acttgaaata gtgggcggaa
      841 aatacaagcc acttatatta tggcacttat cgggtaaaac attacgtttc gggcaattac
      901 aaaaattgct ttcatctgca acgccgaaaa tacttacaaa acagctacgc gagcttgaaa
      961 atgacggact gattgcacga aaagtttatc ccgtagttcc gccgaaagtg gaatattcac
     1021 ttacgccgct cggaacaagt tttattccta tattatcaag catttgcgat tggggaaata
     1081 attatttaaa ggcaaacaac atttgtccta actgttctat gacaaaacaa aaataacaaa
     1141 aaggagctga tttcagctcc tttttgtgtc agtttaatta aaccaatata ttaaagaacg
     1201 tcgttcttta taaggtcctc atatgattca cgctttacaa ctaccttagc tttaccgtcc
     1261 gaaacagcaa caactgccgc tctcggaatt ctgttataat tgcttgccat tgaatagtta
     1321 taagcacctg ttgcaagaac tgccaaagta tctccgactt tgatttccgg catcatcgca
     1381 tctttaagca aaatatctcc ggattcacag cacttacctg ctactgtaac cttttcggta
     1441 cgttcggcat ttgcgttgtt cgcaacaacc gcctcgtact ctgattcgta aagtatatat
     1501 cttgggttat cacccatacc gccgtctacc gacacatact ttctcacatt cttaatatcc
     1561 ttaattccgc ctactgtata aagcgtaata cccgccggcg caataattga acggccaggc
     1621 tccataagaa tgaatggtag tttgactcct cttttttctg cagtagcctt aacaacctct
     1681 gaaacgtgct gaatatattc atcatacggt acaggatcgt cattttcggt atacataata
     1741 ccgtatccgc caccaaggtt aagattatca atttcaagac caagcttgtc ctttaagtcg
     1801 cctacaaagt tcatcattat ttcagccgct ttacagaacg gttcaatatc aaaaatttgt
     1861 gaaccgatgt ggcagtgaac accgtttact tttacgttag acattttact tgctttttca
     1921 ataatttcaa atgcttcgcc attttcaagt gcaacaccga actttgaatc aatttgacct
     1981 gtttgaataa agctgtgagt atgagcgtca acaccgggtt tgattctgaa cattatgttc
     2041 tgaacaacac cgtatttctt agcagtttcg ttaagtgctt caagttcata aatattatca
     2101 actatgatat taccaacttt atttttaact gcaagctcaa tttcatcagc agttttatta
     2161 ttgccgtgaa aatagacttt atccatagga aaatnnnnnn nnnnttatcc atcgcgtttt
     2221 tataaacacg acaatttttt cttattaaat cctcatctaa tacatatagc ggagttccaa
     2281 actcctttgc aagttcaaca gtatcatttt taccaataac aagatgattt ttctcgttta
     2341 ctgtcaaatc ttctgataca aacattcttt ctcacacctt cctaaagttt cttaaccctg
     2401 agttccgtca ggctctaaat tttctttgag tttcttcgtt tccttagttt cgtgtataat
     2461 cttaggttct tttaaatctg ttatacactt ctttgttatg ataacggttt ttatactgtc
     2521 atcggacggc acttcaaaca taatgtcaga cattgcctcc tcgataattg aacgaagtcc
     2581 tctcgcacct gtatttcttt caatcgcttt atcagcgatt gcatctatcg cgtcatcttc
     2641 aaattcaagc tttacaccgt caaatgcaaa caaggcttta tactgcttta taagtgcatt
     2701 tttcggttct gtaagaattg taataagcgt ttttctgtca agtttatcaa gtcttgcaaa
     2761 aacaggaagt ctgcctataa attcaggaat caatccgaat ttaagcaaat cctgcggtat
     2821 aagtttaccc agcaattcat tgatgttaga ctctttcttg cttgaaatct gtgcaccaaa
     2881 acccaaacct tgcttacctg ttcttgagcc gataatcttt tcgataccgt caaaagcacc
     2941 gccgcatata aacaaaatat tcgttgtatc aatctgaata agttcttggt gcggatgttt
     3001 tcttccgccc tgcggaggaa ctgacgcaac agtgccttca agcactttca aaagtgcctg
     3061 ttgaacacct tcaccgctaa cgtcacgcgt aattgacgga ttttcggatt ttcgtgcaat
     3121 tttatcaatt tcgtcaatat atataattcc gcgttctgca gcgtcaatat cgtaatcagc
     3181 cgcttgaata agttttagaa gaata
//
LOCUS       DAWACS010000131         3204 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3219_length_3204_cov_2.681169, whole genome shotgun sequence.
ACCESSION   DAWACS010000131 DAWACS010000000
VERSION     DAWACS010000131.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3219_length_3204_cov_2.681169"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    222..231
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1008 a          624 c          605 g          957 t
ORIGIN      
        1 tcttctgatt gtgttaagtt caggtgacgc atcgtcggca atttcatttt cagaaacgat
       61 acacgaatta attctgtcct caagttgttt tgccgttatg atagcctcct ctataccgtg
      121 aagaattgta cattcttcgg ctgattcgtc tatatacgac ttcatacgtc ttgctacata
      181 caaaagtctt gatatgttca taagttcctt tgtatgaagt annnnnnnnn ncattttcaa
      241 cagacagatt taccggcggt gagccgagct ttaataaggt tgacattgct tctgtcgttt
      301 ctttctgtgc cgcccttgca tcctctaatt ccgtatacgg aactatttct tcaatgcgtt
      361 tttttacgtc tttgctctcc gtatatgatg aaagccgttc aagtatcttg tcaaattcaa
      421 gtaccttgaa cgctttattc tgtttatcca ttttacctct cctattttat atagaatata
      481 cttttagatt agaatactca ccaatcatag gagccatttg tctgaaacct atttttccgc
      541 cgcctaaaac aggaccatat tcctttccgt cgtcttgcca tgaaaataca ggcaaatcgt
      601 taattataaa atcaatctta ccgtcttttt tcacaagctt tatatgatag ctttcaaatg
      661 cgtcctcaca attcggaata gggtctgcac cttgtacaac aagatgaaag cccttgcttt
      721 ttcttaaatt gcaggtatga aaacctcttt cctcgtccca ctttctgcgg aaatatgaaa
      781 catggtacgc atttatatca cccgaatgat acaaattata ctgtccgtcc ctttcctgca
      841 atgacggatc aaacaaatct tcaccgttca caccctttgc cgaaaagaac aaaatcgcca
      901 atcccggctc ttcaatcggt ctgaattccc actctatctg cacatctgac gggaagtcct
      961 cgttgcacca atacacaaag tttgactttt gtcccaaatc ggcagacaac gcatttttca
     1021 tacgcatttt accattttca aaatatatct cggcactgcc ctcaagaacg aaatctttta
     1081 cgtcattctc gcaggacaat ttgttttcat aaactaactt cattgttttt aatcctccac
     1141 aaaaaactca tcttaaccta tattatatca gcaatattta ctaaatacaa cacgaaatca
     1201 aaaaaaagga ttgaaaaatc tgtgagaata gtatagaatg gtatatatat ttttatgtaa
     1261 agcaatataa cattgcgagt ggacaataag gaggtacatt ataatatgtg cggtatagta
     1321 ggatatatag gtaaaaatca agcagctcct attttgcttg aagggctgtc aaaacttgaa
     1381 tacagaggat atgacagtgc aggtattgct gtttacaata ataatgaatt ggaagttgct
     1441 aagtcaaagg gaaaattagc ggttctttca gaaaaaattg acggcggtaa aaaacttagc
     1501 ggtaatatgg gtatcggtca tacacgttgg gcaactcacg gcgagccgtc agacgtaaat
     1561 gctcaccctc acctttcaca atcaggtcgt tttgccgttg ttcataacgg tattatcgaa
     1621 aactacattt cattaaagaa aaaacttgaa agcaagggtt ttgaatttat ttctgaaaca
     1681 gatacagaag ttatcgctca cctttttgaa tattactaca aaggcgatat aatggataca
     1741 atgattaagg ttatcaacag agttgagggt tcatatgctc ttggcgtact ttgcgttgac
     1801 taccctgacc aatttatcgc agtaagaaaa gcaagtccta tgattgtcgg tcttggtgac
     1861 gaagaaaact ttatcgcatc ggacgttact gcaattttaa agcacacaag agatatttac
     1921 taccttgaag ataacgaaat cgttgtactt aaaaaggaca gcgtaaaggt ttacaataca
     1981 gataaagaag aagttaaaaa agaaaagttt acggttgatt gggacgtaag tgcggctgaa
     2041 aaaggcggct atgaacactt tatgatgaaa gaaatcgaag aacagccaaa agctatcaga
     2101 gataccatca gtccgagaat taaggacggc aaaatcgttc ttgacgatat ttctcttaca
     2161 gaagaagata ttaaaaatat caataaaatt tatatcgttg catgcggcag tgcataccat
     2221 gtaggcgttg taggcaagta tgtaatcgaa aagatgtgcc gtattcctgt tgaagtacag
     2281 gttgcgtcag agttccgtta ctgcgaccct atcgttggca aagacgacct tgtaatcgta
     2341 atcagccagt caggcgaaac agccgataca aaagccgctc ttgaagaggc gaaagctcgc
     2401 ggcgcaagag tgctttcaat cgtaaacgtt gtcggcagtg ccattgcaaa agcgtctgat
     2461 gatgttattt atacatgggc aggtcctgaa atcgccgttg caacaactaa ggcctacagt
     2521 acacaactta ctgttatata ccttatcgcc gcatatatgg ctgataaatt aggtaaaatt
     2581 tctaaagaag aatatgcaga ctttattaaa gaaattgagt cacttccgga taaggttgct
     2641 gaaattctta aatctaaaga agatgttcag tatcttgcct caaagttcta taattgccat
     2701 tcaatattct ttatcggcag aaaccttgac tatgcagtat ctcttgaagg ctcactaaag
     2761 ctaaaggaaa tatcatatat tcacagtgaa gcatacgccg caggtgaatt aaagcacggt
     2821 acaatctcac ttattgagga cggtacactt gttgtagcgc ttgcaacagg caagaatttg
     2881 tttgataaaa cagtttcaaa tgttaaggaa gttaaagcaa gaggtgcggt tgttatgggc
     2941 gtaacaactg aagaacatga acatatggac gacgttgctg actataccgt aaaaatccct
     3001 gcaacgcacg aaatgttact tccgtcactt acagttattc cgttgcagtt attcggttac
     3061 tatgttgcat cattaaaggg ctgcgacatt gataagccga gaaaccttgc aaagtcggtt
     3121 actgttgaat aatcctgaca gaaatatttt ttgtggtata ataataattg atagagtgcc
     3181 tgacaggctt tgaaggtcag acac
//
LOCUS       DAWACS010000132         3203 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3220_length_3203_cov_2.071156, whole genome shotgun sequence.
ACCESSION   DAWACS010000132 DAWACS010000000
VERSION     DAWACS010000132.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3220_length_3203_cov_2.071156"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1075..1084
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2639..2648
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2978..2987
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          958 a          627 c          656 g          932 t
ORIGIN      
        1 ttttcgccga caatcatacc ttcgtaaacc ttaacgcccg cacctacgaa caaagtacct
       61 ctttcctgtg cgttgtaaag accgtaagta atagttgtac cgtcctccca agcgataaga
      121 gtaccacggg ttctgccgtt aaagtcacct ttgtacggct cgtatttttc aagaatactg
      181 tttatgatac cagtaccctt tgttgcggta agaagttcac ttctgtaacc gataagacct
      241 cttgacggaa ttaaaaattc aagtcttgtg tatccctgtg cagtcggtgc catatttgtc
      301 atttcacctt tacggtttat aacaccgtcc attacagtac ctgtgtattc atccggaacg
      361 tcaactgtaa gtctttcgat aggctcacat ttaacgccgt caatttcttt ataaataaca
      421 acaggatttg aaacttggaa ttcataaccc tcacggcgca tattttcgat aagtactgaa
      481 aggtgaagtt caccacgtcc agaaacgata aagctttctg tcgaatctgt ttcgtcaact
      541 ctaagactta cgtttgaatc aagttctctg aacaatctgt cacgaagatg acgggaagta
      601 acgaatttac cgtcttgacc tgcaaacgga ctgtcattaa cgctgaaagt cattgaaagt
      661 acagggtcat cgatttttac gaacggaagt gcttcgggat tgtcaacgtc acaaaccgtt
      721 tcaccgatat taatatcagc gatacccgaa attgcgacaa cgtcaccggc accgacttcg
      781 tctgtaggaa cttttttaag tccttcaaaa gtaaatagct ttgtagcttt tcctcttgtt
      841 actttaccgt catgacggca gatcgaaagc ggcatattaa cctttatact gcctctttcg
      901 attttaccta ctgcgatacg gcctgtataa tcatcatagt caatgtttga aacaagcatt
      961 tggaacggtg cgtcgtcctc gcatttaggg caaggaattg aatttacgat aagttcaaac
     1021 aattctgtaa ggtcattgtt cggttgttcc ggattgtatt ctgcggttgc ccaannnnnn
     1081 nnnncgttcg gtctgtcaac cttgtttaca acgataacgg gctttaggtc aagtgccaat
     1141 gcctttgaca atacgaaacg tgtctgcggc atacagcctt caaatgcgtc aacaagcaaa
     1201 agtacgtcgt caaccatttc cagaccgcgt tcaacttcac cgccgaaatc ggcgtgtccc
     1261 ggtgtatcaa taatatttat tttaacaccc ttgtagtaaa gagaagtatt ctttgcaagg
     1321 attgtgatac ctctttctct ttcaaggtcg tttgagtcca taactctttc gtccacttgt
     1381 tcgttttcac ggaatgtacc gctctgtgca agcattgcgt caacaagagt tgtcttaccg
     1441 tggtcaacgt gggcgataat tgcgatattt cttatatttt cacgttttat cataattaat
     1501 ctcctaatct cctcatttta ttactctaac ctctttatta tatacccgtt ttgcaattat
     1561 tacaatattt ttgccgaaaa aacagaataa tgtacaaaac cgaccttgca taaacactgt
     1621 ttaaatgtta taataacaag tgatttaata aatgaggagt aaagataatg gattttaaag
     1681 aacatataat tgaaaaaatt gttgatataa caggacttga aagagaagtt gtggaaaaat
     1741 ccgtcgaagt tccgcctgat gaaaaaatgg gcgacatcgc atttccgtgc tttccgcttg
     1801 caaaggttat gagaaaagca cctcctatta ttgcgcagga gcttgctgaa aaaatgtcgg
     1861 gtgacgatat gattgaaaaa gcacaggctg ccggcggtta tttaaacttc tttttaaacc
     1921 gtgaaaagtt tatttccgaa acagtttctt ctgcacttga cgcaggcgag gattggggca
     1981 agtcggatat gggtaagggc aagactgtac ttgtcgaata ttcgtcaccg aatatagcaa
     2041 agccgttcca tatcggtcat cttttctcaa cggctgtcgg aaattcactt tcaaagattt
     2101 acaagcacct tggatatgac gttcagtcgc ttaatcacct tggcgactgg ggtacgcagt
     2161 tcggtaagct tatatgtgct tacaagagat ggggtgttaa agaggttatc gagaaagacc
     2221 ctataaacga gcttttaaag atatatgtaa aattccacga agaagctgaa aagcaccctg
     2281 aacttgacga tgaagcaaga ggatatttca aaaagcttga ggacggtgac gaggaaacaa
     2341 cggctctgtg gacatatttc agagatataa gccttgttga attcaagcga gtatataata
     2401 tgcttggcgt taagtttgat tcatataacg gcgaagcatt ctattcggat aaaatggacg
     2461 aagttgtcga tatacttcgt gacaaaggat tgcttaccga aagtgacggc gcacaggttg
     2521 ttgacttgtc agagcttaac ataccgcctt gtatcgtact taaatcggac ggtgcgacaa
     2581 tttatgcaac acgtgatatt gcggcggctc tataccgcca cagaacatat gatttctann
     2641 nnnnnnnnat tatattcagt tgggatacta tgcttgactt tgagggcgag tctgctccgt
     2701 attgtcagta cggttatgca cgaggcagaa gtattttgag aaaagcagag ggtattgatt
     2761 actcaaacgc agacttctca aaagcagcat ctgatgaggc atattcgctt gtaaagcaaa
     2821 ttaacggatt cggtgacgcg gtaaaagatg cggcagacaa gaacgagccg ttctatataa
     2881 acagatacgt tacaaatctt acaaagtcat tcaacaagtt ctacaacaca aatcctatta
     2941 tgaaagacga tgtggatgaa gaaacaaaga aagcacgnnn nnnnnnncat tgggattgct
     3001 tggaattgaa actgttgaaa gtatgtaatt tataaaaggg tgttgtatgt gcaatgatat
     3061 attcgcaaaa tataaataaa gtgtgtgccg tctgtcagat ggctcataaa aaaagcgaag
     3121 atgaaatgta ttgtgatata aaaaaacaag cggtgtccgt taccgatacc gcttgtgata
     3181 aattcaaata cgatatttta aaa
//
LOCUS       DAWACS010000133         3161 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3266_length_3161_cov_3.014166, whole genome shotgun sequence.
ACCESSION   DAWACS010000133 DAWACS010000000
VERSION     DAWACS010000133.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3266_length_3161_cov_3.014166"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1004 a          581 c          663 g          913 t
ORIGIN      
        1 aatctttgaa gaaaccttac ttccgccaag aatagctaca agcggtctta cagggttgtt
       61 tactgcgtta ccgaggaagt tgatttcctt ttcgattagg taacctgcaa cagccggctt
      121 aagaattgaa gatacaccta cgtttgagca gtgagctctg tgagctgtac cgaatgcgtc
      181 atttacgaac aaatcagcaa gtgaagcaag ttcttttgaa aaagcttctt cgttctttgt
      241 ttcttctttt ctgtaacgag tgttttgaag aagaacaaca tcaccgtcat tcatttctgc
      301 aacagccttt ttagcgttgt cgcctactac gttatcgtca tcggcaaaaa caacttcttt
      361 gccaagcttt tctgaaagac tctttgcaac aggtgcaaga gaaagtgatg gaacaggctc
      421 accctttggc ttacccatat gtgagcaaag aataactctt gctccgttat ctataagata
      481 cttaattgta ggaagtgcac ctacaatacg gttttcgtct gtgatattac cgtcagcgtc
      541 aagcggcacg ttaaagtcac aacgtactaa aacctttttg ccttttacgt caacatcttc
      601 aactgttaat ttgttgtaca tagtcaattt ctcctttttt atgcggacaa aattatccga
      661 tttttcttga ttatattata tataaaaatt atctgttttt caagtatata attaaaattc
      721 gttaaaattt taccaactta atcaaaatag ccgccgtcaa tatgccaact atgtcaccga
      781 cagccgcaaa aggaacagct ttcaagttat gttttaccct tgttttcgca aaatatacgc
      841 aaagacagta gaaagttgtt tcagtcgaac ccattataac ggaggcaatt cgaccgactt
      901 cgctgtcggc accgtaattg tttaaaattt cggtaagtat gcccgttgca ccgctgcccg
      961 atattggacg gataagtgcc aaaggcataa cttcggcagg aattaaattt gtgataggtg
     1021 aaagaagtga tactataagg tcaagcgtgc ctgacgcacg gagcatatat gcggcggtaa
     1081 gcaccgctac aagcggcgga aatatgcctg atactatttt cattccgtcc tctgccccat
     1141 ctataaaaag ttcgtatgac ggaagttttt ttatcattgc ggttataaga aaaagtgcaa
     1201 ttatgagcgg tattacataa atcatatttt actccctata tagtaatttt gtcagtgtta
     1261 aagcaataaa tgcggcggtg aatgaagaaa tccaaatcgg cagaataacc gaaaacggat
     1321 ttacagagcc tgccgcaaca cgcagagcga ttattgttgt gggtataagc tgtacggacg
     1381 tggtgttcaa aacgacaagc atacacatat tttttgacgt ataaagcgga ttgttgtttt
     1441 ccttgtcaag acattgcata gcctttatac ccataggcgt tgcggcattg cccattccga
     1501 gtatgttggc actcatattc acggcaatat atttttttgc gttttgacct gcattgggaa
     1561 acaggaagtt tattataggt tttaataatt tctctaattt ctgtgacaat cccgcatttt
     1621 cggcaatttt cataataccc gtccaaaagc acataactcc cgcaaatgac aataccgtaa
     1681 aaacggcact tttcgcaccc tcaaacgcac cgtttatagt ttggtcgaca gtgccgttaa
     1741 aaattgacac aactatcgac acaataatca tcaagcacca aatataattc atatactttc
     1801 ctccgtttct tgtgaatttg atatttattt atatgaaaaa ttgtgctata atagaagaat
     1861 gtatataaat ataatttgaa cggaggatta gtatgctcaa aggaaaaaca gtcgtgcttg
     1921 gagttacggg aagtattgcg gcgtacaaaa tcgcaaatct tgcaagaatg cttgtaaaac
     1981 agcattgtga agtacacgtg cttatgacgc agaacgcaac taattttata aatcccataa
     2041 cttttgaaac attgacaaag cataaatgcc ttatagatac atttgaccgt aattttgaat
     2101 tcagcgttga acacgttgca cttgcaaaag cggcggacgt ggttatgatt gcaccggcga
     2161 gtgcaaatgt aatcggcaag attgcaaacg gtatcgccga cgatatgctt acaaccactg
     2221 ttatggcttg tccttgcaag aaaattgtgt cacctgctat gaatcataat atgttccata
     2281 accctatcgt gcaggataac attgaaaagc tgaagcatta cggctatgaa atagttgaac
     2341 cggcgcacgg tatgcttgca aacggtgata tgggtgacgg cagaatgcct gatgaagagc
     2401 ttttgtttga atacattgtt aaagagattg catttgaaaa agatatgaca ggtaaaaaag
     2461 tgcttgttac agccggtgca actgtagagg ctatcgaccc tgtgagattc ataacaaacc
     2521 attcgagcgg aaaaatgggt tttgcacttg caaagaacgc aatgctaaga ggtgcggatg
     2581 tgacgcttgt tatgggtaaa tgcgacagtg agcctcctgt gtttgtgaat acggttaagg
     2641 tgcagagtgc gaaagatatg tatgacgcgg taatagaacg tgccgacagt atggatataa
     2701 tcgttaaggc ggcggcagtg gctgattaca gacctaagaa cgtaagcagt gaaaaggtaa
     2761 aaaaacagga cggtaatatg tcgattgagc ttgaacgtac ggacgatata ttaaaaactc
     2821 tcggtgaaag aaaaaacggt caatttatct gcggtttctc tatggaaaca gaaaatatgc
     2881 ttgaaaattc aagaaagaaa cttgaaaaga aaaattgtga tatgattgtc gcaaataatc
     2941 ttaaacaaga cggtgcaggt tttggcggta atacaaatat cgtgacgctt ataacaaaag
     3001 atgatgaagt tcagcttgaa aagatgacaa aagatgaagt tgcggaaaag atatttgatt
     3061 tcattttgag tagataggaa gtagattatg tacggatacg aaacgttcca cgaggatttg
     3121 atgaaaaatt taatagaatc ggtgcataac ggcgtaagct c
//
LOCUS       DAWACS010000134         3157 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3273_length_3157_cov_1.918762, whole genome shotgun sequence.
ACCESSION   DAWACS010000134 DAWACS010000000
VERSION     DAWACS010000134.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3273_length_3157_cov_1.918762"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          917 a          747 c          484 g         1009 t
ORIGIN      
        1 cttttaggta ctgaaaaaac ccctgaatat attatagtac ctacatttga cagcgacccg
       61 tatgtttcct caccgcagtt tgcctcactc ggaaacaccg aaaaaatctt aaaaagtctt
      121 aacattgtga tgaaaattct taatacaaaa aaggcaatta tagccgttga aaataataca
      181 aaaaagatat tttccgattt caaactaaat cttagatata acgaaaatat ttctctctgc
      241 tcgctcaaag cacgttatcc gcagagcaga gatgacattc ttgtgaaaac gcttacaggc
      301 aaggatattg acaaaataaa taccgtcata ctgtcacccg aaacactttg caacatctat
      361 gacgcactta cacttcaaaa gcctgttacg gaaaaaatta taacggtttc aggcgacgat
      421 attcttccgc cgaataatta caaagtgccg ataggcttgc ctgtttcgtc acttcttcac
      481 gacagcggat atacttctcc cgaaacggtt atagtcggcg gtatcgttga cggtcgtcaa
      541 atcaccgacc ttgacgagcc gataacatca agtacaaatt caatcattgc atttaacgac
      601 agttcaaata ttcccaaata cagaaaagaa cttatctaat cagataagtt ctttttcttt
      661 taaaacttta taaagcaatt cacatgaaca ttcaacaaaa ttctgaatat cttctttttg
      721 tgtatattca aattctctgt ccgtatttga actgtagctt tcgtaataat caacaataaa
      781 cttaacctgt tttgtaagct gattatgctc gtaatacggc agataatctt ttatttcacc
      841 cgcatttttc agtatatcaa aagtcggatg atatgtaaga cttcttaaat gccatacgtc
      901 aatatcattc cagtcctttt ttataacctt taaaaattcg gggtttttct tatattcctc
      961 cgcatattta atccttgtcg gcatatacat cgtaaccgac cacattatat ccgtcagcaa
     1021 atgcacataa tacccaaggt aaaaccaata atcggcgttt gatttatcgt ttagatattc
     1081 cttttggaag tcgttatatc ggcaatcacg tttcataccg ctcggtgacc aatgagttat
     1141 tttaggcggc ggtgtgaact caccaaaact gtccttaaca ccgtatccgc agtcaggtgc
     1201 aacactgcct atgacaaact ctctatgata ttttttagct attaaattat caataaaaca
     1261 ccttgccact cttaaatgaa ctccccaagt tgccaatata atacacctcc gattaccact
     1321 gagctattgc tatattatac aaaagtttaa tgctgttttc tatgtcactc atgcttgcca
     1381 tttcagacgg tgaatgtaca tatcttgtcg gtacggaaat accgcccgtc ttaattcctg
     1441 ctcttgtaag atgaattgca cctgcgtctg taccgccgtc cgtcataatt tcaagctgat
     1501 acggaatttt gtttttctta gcagtttcaa tcatcattgt gcgaacatcg gcatcacaca
     1561 taatcgaacg gtccataacc tttatagccg tacccttacc gagttcaatc gccatagtcg
     1621 gtgcggacgg tgtatcgccc gtatcggtaa cgtcaaccgc taccgcaata tctggattga
     1681 ttgcaaaagc tgccgttttt gctccgcgaa gtcccacttc ctcttgagtt gtaaacacga
     1741 aatacaagtc gttttcacaa tcttttattt cactcaaagc ctgcattaaa atcgcacagc
     1801 cgactctgtt gtcaagagcc tttgaaacaa cattatcgcc acacttcaca aatccgccga
     1861 caaatgacgc ggtatcaccg attgaaacta ttttttcagc ctctttctta tctgccgcac
     1921 ccaaatcaac gtataatttg cttatagccg gtttatcttt aaaactttct tcctctgcac
     1981 cgattacgcc ctcaacgccg tttgcaaaac gcacacgaag atttacaaga tttctcaaat
     2041 taagtccgcc gactgacgaa aaacgcacaa atcctttatc gtcaatatcg gttacaatta
     2101 taccaatctc gtccatatgt gacgcgaaca taatcttttt accactgcct tttttatgag
     2161 cgataagatt gcccattgcg tcaaccgaaa gttcgtcaca atactttttt acattatcgg
     2221 aaataaactt tctgatttca ttttccgagc ccgaaacact gtttatttgt gttgcttttt
     2281 ccaatatatt taaaatatcc attatatcat ttctccgatt ttatttaaaa ataatctgat
     2341 atactccgtc atactgttaa tatcatcaag tgacattacg gaaacaggcg aatgagaata
     2401 tctgcaagga acagacgaac tcaggcatat cgcaccgtcc gctccgctct gcattgcacc
     2461 gccgtcactt gtctgtggca ttaaaacctg aggctgtacc tttattttcg cctttttagc
     2521 ggctttaatc attttttcgg ttatttcttt atccgcaatt attgttctgt cttcaaaatt
     2581 aacaaccgca ccgtcaccga gttttacacc cgcattactt tcacaaccgt acatatcggc
     2641 tgtttcggca gatgaaaccg acaatataac atcggcattt attctgtgcg atacgatttt
     2701 cgcgcctctt gctccgactt cacgctgtac caaaaagcac atatatgtgt cgtatttata
     2761 ttctttctca attgcattta taagcgaggt acaaataccg ctcctgtcaa gtgctttgcc
     2821 tttgacgtta ctgccaattt gcataaattc agtgtcaaaa gtcacataat cgccgagttt
     2881 aacgtacttt tccgcatcct ctttgtcctt tgcaccgatg tcaataaaca gctttgaaac
     2941 tgccacaaca ttttcacgtt cggactttgt ctgcaagtgt attgctttca taccgataac
     3001 gcccttgatt ttatcagcac cgattacaac cttttttgaa ataatttttc gcgggtcaat
     3061 attgcctacc gctttaaatt tcaaataacc cttttcggtt actccgctta tgataaaacc
     3121 gggttcgtcc atattaaccg caactgcgat tttttta
//
LOCUS       DAWACS010000135         3152 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3283_length_3152_cov_1.989990, whole genome shotgun sequence.
ACCESSION   DAWACS010000135 DAWACS010000000
VERSION     DAWACS010000135.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3283_length_3152_cov_1.989990"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    328..337
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2063..2072
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2902..2911
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          926 a          602 c          655 g          939 t
ORIGIN      
        1 gatatgacgg taaaacgtat tgctttaaag agtattcggt cggagaagac gattataaaa
       61 tgtttgtctg ctatttgtcg tagttgtata tgtttgcact ttcgggtgag tagtttataa
      121 tttcgtcata agagtcaagc tgctcttcgc tgttcggtgc ccacggaata agcccggtac
      181 agtcagttgc ggatatggca ttgcatacgt caaagtcaaa attactgtta cggaaaataa
      241 cgtgattcgg atatttcctt ttcataacat cataaccttt ctgctcaaat ttagtttgga
      301 gcagtattag tattataaaa atcccgannn nnnnnnncta tatgaccgtt cagcttacac
      361 aaaattttta cgttatcgcc gacactgtca atccacggcg cacggataaa tacaagcgga
      421 aacggtttat ccgatacttc ggggataacg acttcttcgg aaaaactgtc gatttgcttg
      481 ccgtatgcgt tgcgccttac agtaatatcc atagtaccta tgtgtgcctg ttcgccctca
      541 atatttttcg caagaacgat aagtccggca caagtaccga aaacaggcat accgttttca
      601 atcatatttt taatcggctc atataaatcg agtttgtgaa gaagtttgcc gattaccgta
      661 ctttctccgc ccgggataat aagaccgtca aacggttttt caatgtcttt tttttgacgt
      721 atttcaaatg attccacacc aagtgcgtca agcgaatgtt tgtgttcaat aaatgcacct
      781 tgaagtgcaa gtataccgat tttcattatt taccacgctc cgccataagt agtgcaattt
      841 cgttttcgtt tatgccgacc attgcttcgc caagatcctc tgaaagctct gcaagaagtt
      901 tgtcgtcagt gtaatttgta acggcttgaa caatggcaga cgctcttttt gcagggttgc
      961 ctgacttgaa tatacccgag cctacgaata caccctctgc accgagatgc atcataagtg
     1021 cggcgtcggc aggtgttgca acaccgcctg cggcaaagtt tacaacaggc aatttaccgt
     1081 tttcgtgaac atattcaaca aggttgtaag gcacttgaag ttgctttgca gcctcataaa
     1141 gttcgtcttc tgacatattt tgaatacgtc tgatttcaga catcatcatt ctcatatgac
     1201 gtactgcctg aacaacgtca cctgtacccg gttcaccctt tgttcttatc atagccgcac
     1261 cttcgtttat acgtcttagc gcctcaccca aatccttagc accgcatacg aaaggtactt
     1321 taaaatcgtt cttgttgatg tggtacttgt cgtcggcagg tgaaagcact tcgctttcgt
     1381 ctatgtagtc aattttgatt gcttgcaaaa ttcttgcctc tgcaaagtga ccgatacggc
     1441 acttcgccat aacagggatt gaaactgcgt cttgaatacc cttaatcatt ttagggtcgc
     1501 tcattctcga cacaccgcct gcggcacgaa tatcggcagg gatacgttca agagccatta
     1561 cagcacaagc gcctgcctcc tcggcaattt ttgcttgttc aggtgttgtt acgtccataa
     1621 ttactccgcc ctttagcatt tgggcaaggt ttctgtttag ttccatttgt gttgacatag
     1681 ttattaacat tcctttctgc tcggcttaaa taaatgagcc gtctgttgac tttttcactg
     1741 ggattggtta taattatacc actgattgac actattaaaa gtgccaaaat acaagttttt
     1801 tatggggtca gattatgttt aactttattt ttacagccga taaagcgtca aaaaaaccgc
     1861 tttacgaaca gctttattcg tatgtcgcag atgaaataaa aaataaaaat attaaagaaa
     1921 atgagcgtat gccgtcaaaa aaagcactgg caaaatatct cggaataagc gtaaataccg
     1981 ttgaaacagc atattcgatt ttggtacagg aggggtattt aagagcagtt gcaaggagcg
     2041 gtttttatgt ttgcaaggtt gannnnnnnn nnaggttgat attcccgttg gtgacagaaa
     2101 ctttgaatat gaagaagaac aagatagtgt aacggcatat aaggcggatt ttaagacaaa
     2161 taaagtcgat ataaattcgt ttccgtattc gacttggata aagctttcaa aggaggttat
     2221 gtactcaaca ccggagtacc ttaattccgg aaatgtaaag ggcgattatg aattgagaga
     2281 gagtattgca aaatatttgc acgaatttcg cggagtaaaa tgttcgccta tgcagattgt
     2341 agtcggtgcg ggtattgagt atttgacaat gctccttaca gaactttttg acagagataa
     2401 agtgtttgct gttgaaaatc ccggttataa aaaaatatcg gcaatactca gaaacaacaa
     2461 cagaaaaata aattatatag acgtagatga aaacggactt tgtacggatt gtttaaaaca
     2521 aacagattcg gatatagtgt atgtcacacc gtcacaccaa ttcccgacag gtgcggtaat
     2581 gcctgtatgg cgcagaatgg aacttctttc gtgggcggaa gaaggggagg acagatatat
     2641 aattgaggac gattataaca gcgagtttaa tttttcgatt aagcctgttc ccgcaatgca
     2701 gggacttgca agcagtgata aagtcgtgta tttaagcaca ttttcaagag ttcttgcacc
     2761 gtcaatcaga attgcatata tggtacttcc gaaaaaattg cttgaaaaat tcgacaagca
     2821 tttcggtaca tattcgtcaa ccgttccgcg ttttgaacag catacgttaa ataaatttat
     2881 ttcgggaggg tactattcaa gnnnnnnnnn nacgcgataa gattatccag tcgctcggta
     2941 acgcagatat aagcggtgaa agagtcggac ttcatatagt cgttaaaacg gataaggcaa
     3001 aggaaataat agagtatgct ctaaaatgtg acgtgaaact ttacgatatg gacgattatt
     3061 atatgactga aaataaaaaa agcaattcaa tcatcatagg ctatgccggt gcggaagatg
     3121 atgatattga attactgaaa agtatatttg at
//
LOCUS       DAWACS010000136         3146 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3288_length_3146_cov_2.301197, whole genome shotgun sequence.
ACCESSION   DAWACS010000136 DAWACS010000000
VERSION     DAWACS010000136.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3288_length_3146_cov_2.301197"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2881..2890
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1050 a          499 c          779 g          808 t
ORIGIN      
        1 aaggcaagac ttgcacaggc tacaatttat gtcgaagttt ttgatgaaga caaaatggat
       61 attgtagtgg aaagattgtc gaaaatattc ggtattgttt caattacaag agcggttgtt
      121 tgtgaaaagg atattgagga tataaaggcg aaagccaagg aatatttgaa gaaagacttt
      181 aaggacggcg ttaaatttaa ggttgaggca aagcgttctg ataagcagtt cccgcttaac
      241 tcacctcaaa tttgtatgga agtcggcggt tatcttgacg acgaatatcc gaatatagtc
      301 gttgatgttc ataatcccga agttacggtt aaagtcgaag tcagagattt cggtgcatac
      361 gtttatgcag atgaaaataa agtgcaggga cagggcggta tgccgatagg cacaggctca
      421 aaggcaacct tgcttttgtc gggcggtatt gacagcccgg ttgcaggtca tatgatttca
      481 aaacgcggtg ttgaaattga tgcggtaaac tttttctcat tcccatacac aagtgagaga
      541 gcaaaagaaa aggttattga acttgcaagc ataatcgcac aatatacgtc aaaaatcaat
      601 ttgtatatcg tgccgtttac ggaaatacag ctgcagattg cagaaaagtg ccctgaggaa
      661 catttgacgc ttgttatgcg tagatttatg atgcgtattg cggaaagatt ggcgcgtaag
      721 cataaatcat tggcacttgt aacaggcgag agcgtcggac aggttgcaag ccaaactctt
      781 gcggcacttg acgttacaaa ttcggctgtt gatatgccgg tattgcagcc gcttatcggt
      841 atggataaga ttgaaatagt tgacagagca cacgaaatcg gtacatttga aacatcaatt
      901 ttaccgtatg aggactgctg tacagtgttt acaccaaagc accctacaac aaatccgaaa
      961 cgtgcaaata ttgaaaagtc tgaaaataga cttgacgttg aaacacttat cgaggctgct
     1021 ttggcaggcg ttgaaaagat agaagtatat cctaaataat aaggcttggg tgattgatat
     1081 gaaaggggaa gtaaacggtt taaatgaaaa agttgtaaaa atgcttgcgg aaagaaaaat
     1141 gactgtttcg gcggctgaaa gctgtacagg aggacttttt gcggcactta taacaaatgt
     1201 ttcgggtgcg tcggaggttt tgaatgaaag ctttgtgact tatgcaaatt cggcgaaaat
     1261 gaaatatctc ggtgtcaagg gcgaaacgct ggaaaaacac ggtgcagtga gttatgaaac
     1321 cgcttttgaa atggcagatg gactcagaaa aaacacaggt gcgaatgtta ctgtcggcat
     1381 aacaggcata gcaggcccga caggcggaac gaaagaaaag ccggtaggac ttgtatatgt
     1441 cggcatatgc gttatgggtg aaacggaagt gaaagaactt catttaaacg gtacacgcga
     1501 agaagttcgt gagtgtactt gcaaaattac gtttgaaaat atcgcaaacc gtattgaaaa
     1561 tatcactaaa gtacaataaa ttgtactttg cacggcattg agttaaaaac tgcttgaaaa
     1621 aagagtgttt atgtaatata attgacgtat aaaatataag tgatacttta aaggagaaaa
     1681 taaatggcaa aagaaaagga taagagtaaa cctaaagttg taaaaatgac tgacgcagag
     1741 gaaaagaaaa aggctcttga atctgtattc agtgttatag aaaaggaata cggtacagga
     1801 agtattatga aactcggcga cgcaaattcg gttgacgttg aagttattcc gacaggctcg
     1861 ttgacgcttg atatggcact tggagtaggc ggacttccga gaggcagagt tatagaaata
     1921 tacggccctg aatcttcggg taagacaacc gttgcacttc acgttgtggc agaggcacag
     1981 aaaatgggcg gtgaggctgc atttattgac gcggaacacg ctttagaccc tgtatatgca
     2041 aaaaaattgg gcgttgatat agacaattta attgttgcac aaccggatac cggtgaacag
     2101 gctttggata ttgccgaggc acttgtacga agcggtgcgc ttgacgtaat cgttgtcgac
     2161 tctgttgccg cacttgttcc gaaagcggaa attgacggtg aaatgggtga cagccatgtc
     2221 ggtttgttgg caagacttat gtcacaggct ctaagaaaac ttactgccgt catctcaaaa
     2281 tcgggtacgg tcgtaatctt tatcaaccag ctaagagaaa aagtcggtgt tatgtacggt
     2341 aatcccgaaa caactccggg cggtcgtgca ctaaagttct tctcatctgt aagattggac
     2401 gtaagacgcg gcgaggttat aaagaacggt acggaactta tcggtaacaa gacaaaggtt
     2461 aaagttgtta aaaacaaggt tgcaccgccg tttaaaactg ccgaatttga tattttgtac
     2521 ggcgagggta tttcaaaaga aggtaatatt cttgactttg cagttgaaaa caatattatc
     2581 aagaagtcgg gtgcttggtt cagctataac ggtgaaaaaa tcggtcaagg ccgtgacaat
     2641 gtaagaaaat atatggttga gaacaaagaa tttactgctg aaatcgacag acaggttaga
     2701 gaattgttga aaaataacag cggatacttg ccgagtgagg ctgacgacag tgataatact
     2761 gacggcaaac agccgacgga aacggaaaca accgaaacaa ctgaagaata agcgtgtatt
     2821 atttcacaaa aatattatct gaataataaa aagataagta aataataact aaaaattgtt
     2881 nnnnnnnnnn aaaggattga cgtgggacaa attttttagt atgctaatta taatatttat
     2941 atcggtaaaa tatgtgatta aaatttaaga cagggggcac aattcatggt ttcaagtgaa
     3001 gtggcagttc aaaattcggt aggcttgcat gcaagacctg caacattttt cattcaaaga
     3061 gcaaatgaat ttaagtcaag catatgggtt gagaaagacg aaagacgtgt gaacgcgaag
     3121 agtcttttgg gagttctttc actggg
//
LOCUS       DAWACS010000137         3144 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3296_length_3144_cov_2.292651, whole genome shotgun sequence.
ACCESSION   DAWACS010000137 DAWACS010000000
VERSION     DAWACS010000137.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3296_length_3144_cov_2.292651"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1653..1662
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          939 a          695 c          558 g          942 t
ORIGIN      
        1 gtattacttc atactttgtt gaccaagttg taaatgaggt tatcgccgac cttatgactc
       61 aaaaaggtta ctcggaagat ttcgcaaccc aacaggtata caacggcggt ttgaaaattt
      121 attcaacaat ggatcccgac attcaaaata taatggaagg catatttacc aatacgtcta
      181 acttccctta tacaggcaag ggtgctcaat cggcaatgat tataattgac ccttacacag
      241 gtaaaattcg cggtcttata ggcggtcttg gcgaaaagac tgatattcgc ggttggaaca
      301 gagccactca atcaaaacgt caaccgggtt catcaatcaa accgctttcg gtttacgcac
      361 cggcggttga tatgggtaaa atcacagaag ttgacaccgt tacagacgaa gaaataacaa
      421 tcggcaacga caagtggaaa ccaaagaact catacaacga cttcttaggc gatatgacaa
      481 ttaaagaggc agttgcacgt tcggcaaaca ttcctgccgt aaaggtattg gatatggtca
      541 accttacaaa ctcattcaac tacctacaaa acaaattcca catcacaaca cttgaagaaa
      601 aagataaaaa ctacagttct cttgcactcg gcggtcttac acaaggtgta accgttgaag
      661 aaatggcagg agcatactgt acattcgtaa acagcggtaa atatattaag ccgtatactt
      721 acacaagagt acttgattca acaggtcaag ttattcttga aaacacatca agcacaagtc
      781 aggctatcag tcctgcggca gcatacatta cagctgattt actttctggc gttgtaaaca
      841 gcagcgtcgg tacaggtaaa ggtgcaaagc ttgactgcgg tatttcaaca tacggtaaaa
      901 caggtacaac agacgatgac tgcgataagt ggtttgttgg tttcacaccg tattatgtag
      961 gtgcttgttg gtacggtttc gataatcctg catcaatttc agcggcaggt gtttcgggca
     1021 atccgacagt taccgcatgg aatcttgtaa tggaaaaaat tcacgaatcg cttaacccta
     1081 aagaaatcac aaagccaagt aatgttgttg aggcacaggt atgcgaatac tccggtatgc
     1141 ttgcaactga cacctgccct accacaaccg cttacttcgt tgaaggaaca cagccaaagg
     1201 catattgtga cgcatcacac gcaagcagac gtaataaacc aacgcaaaca cctctatcaa
     1261 cggttgcacc ggttaatact ccaagcgtta tgcctacagt aaatcctgcg gcttcggaaa
     1321 cgcacgaaaa tcaaaacggc aataattcaa atacatcttc aggtataaat tcgggttctt
     1381 cgtcaagtac taacagcggt tcttcgtcaa gtacaaattc gggtactaac agtggttctt
     1441 atgatacaaa ttcgggcagc agtacttcgt caaattcggg cggaactgat tacgaacaag
     1501 gcaatacagg ctcatctgaa agcgaaaata cagcaggcgg agcagaagaa gatacttcat
     1561 cggaagaata agacagcaaa aatgaggctt tttcaaagcc tcattttttg ttactgattc
     1621 agattttata aacagatata cccctacaca atnnnnnnnn nntttcggta attaagaggt
     1681 ttttaaaatc cgccgagtcc gaaacttatt gacaatgcat tattaacact tgccataagt
     1741 ccgtcatcta tacggcctat tttctctcgt aatcttcttt tgtcaatcgt cctgatttgc
     1801 tcaagcaata tcacagaatc cttattaagt ccgtaatcct ttgcagcaag ttcgatatgc
     1861 gtaggcattt ttgccttatt aatttggctt gttatagccg ccgcaatcac cgtaggactg
     1921 tatttattac ctatgtcgtt ctgtataaca agtacaggtc ttataccgcc ttgttcactt
     1981 ccgacaaccg ggcttaaatc ggcgtaataa atatctcctc ttttcacaat caatttactc
     2041 acactccgtc agtttttcct cacacagcga aagactttcg ttatccgcct ccaaacacat
     2101 ttgtgccagt tcaagattta tatccgccat ttccttataa cctttttcaa gttcggctac
     2161 aagtttattt ttatcggatt ttttgatttg tgacaaagca atcaggcccc ctttttcagt
     2221 tttcaagata accgtttatc aactacataa ggtagtttaa taccttcacc gcctttccgt
     2281 cttttgtata aattctcggg atacgcttac ctattacgca tgacacctcg tagtttattg
     2341 tttcaagcca ctcggccaaa tcgtcaacgg ttactccttc acgtccgaat atgattactt
     2401 catcgccttt atcaatatta tggacatttg taacatcaat catacattgg tccatacaaa
     2461 ttcttccgat gataggaact tttacgccgt caacaatcat cttaccatgc tttgcaagct
     2521 ttcttaaata tccgtcagca taaccgatag gtactgttgc aattttggtt ttcttatcgg
     2581 ttatgtactc ttttccataa cttacccctc tgcccggttc aacttctttt atatgtgtta
     2641 ttgttgattt tagcgtcatt gcgggaatta ggtcaagtct tgacttatcc acttcgtcag
     2701 acggatacat tccgtacaaa ataacacccg gacgcaccat atcaaggtgc atttcagggt
     2761 acatcattat tgcggcactg ttgcaaatat gcttaatcgg aatattaagt cctttttctt
     2821 caagtctgct gcaaacatcc ataaaacgtc cgtattgcaa aagtgtatac gatttatcgt
     2881 actcgtcaga tgttgcaaaa tgtgagaata taccttcaat ttcaatataa ggcagttttg
     2941 atatttttat aatttcgtct acaatttctt cattattttc tccggcaaga aaacctatac
     3001 ggctcattcc tgtatcaatt ttgatatgaa ttttcgtaac tttttctttt ctttcagcct
     3061 cataagaaag tgcttttgca aattcatatg tgaatacgct cggcgttata tcaaaattga
     3121 ttaaatcttc aacagtttct tcac
//
LOCUS       DAWACS010000138         3133 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3311_length_3133_cov_2.283951, whole genome shotgun sequence.
ACCESSION   DAWACS010000138 DAWACS010000000
VERSION     DAWACS010000138.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3311_length_3133_cov_2.283951"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2810..2819
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1029 a          626 c          662 g          806 t
ORIGIN      
        1 cgttcactca gaatgtcgcc tttttatttt cgctcaaaac acgcggttga aaatttttta
       61 aaaaaaatgc ctacaatagg caaacctacc gcaaatatat taaagcaaat aaaacgtttc
      121 ttccgcaggc ttgtaatccc tacaacgatt tttgacgata tgggatttat atatatggga
      181 cctgttgacg gtcacaatct aacagaaatt atacaatgtc ttgaatacgc aaaagaagaa
      241 aaacgacctg tctttataca cgtccacacg aaaaagggca aaggttatgc cccggcggaa
      301 aacaatccgc aaaaattcca cggcatatca ccttttgaca aagctacggg tgaaacgaaa
      361 aaaggcagtg aaacatacag tgcaagattt ggcaatacgc ttgtcagatt agcgaaaaac
      421 aacaagaaaa tagttgcaat cacaggcgca atgcctaacg gcacaggact tgacgaattt
      481 caagagcaat ataaagacag attttttgat gtcggtattg ccgaacagca cggtgttacg
      541 ctttcagcag gactggcaac ggtcggatac actcctgtta taccgttgta ttcatcattc
      601 ttgcaacgtg catacgacca aactttgcac gacgtctgtc tgcaaaaact tcacgttgta
      661 ttccctatcg accgtgccgg tatagtcggt gcagacggtg aaacacacca gggtgtatac
      721 gatatttcgt acttatcaca tatgccgaat atgacaattc tttcacctgc cacgctcgat
      781 cagcttgaat atatgcttga ttttgcaatc aataaattca atgcaccgat tgcaatacgt
      841 tacccacgcg gcggaacaga ggctgaaaac gtgccttccg ctttcacttt gggacaagca
      901 cagtgcatac gaaacggctc ggatgttaca attatcgcag caggcagaat ggttaaacgt
      961 gccgaagaag ttgcaaaact tacaacgaaa tccgttgaaa ttttagctat gcctacaata
     1021 aaaccgcttg atacaaagag cataatcgag tcggcaaaaa agacaggcag cgttataacc
     1081 attgaggaca acgtaaaaat cggcggtatg ggaagtatga taggcacatt gcttgaagaa
     1141 aaccatatcg actgcaaatt taaaatattt gctttccccg accaaccgat tacgcacggc
     1201 tcaatagatg aacttgataa attatacgga cttgatgcaa agacgattgc atcaaaaata
     1261 gattaaaagg ataagaacta tgggaaaaat aagacttgac gcacttcttg tcaaaaacgg
     1321 acaggtacaa agccgtgaaa gagcgaaggc tctaattatg gcaggacaag tgtatgtaaa
     1381 aaaccaaaaa tgcgacaaag ccggacagat ggttgacgaa gatacaaccg ttgcggaaat
     1441 tcgcggcgaa actttaaaat atgtaagccg cggcggatta aaacttgaaa aagctatgca
     1501 ggaatttccg attacccttg agggtaaaac cacaatggac ataggtgcgt caaccggcgg
     1561 tttcaccgat tgtatgctcc aaaacggtgc tgttaaggta ttcgccgttg acgtcggcta
     1621 cggtcagttc gcgtggaaat tacgtcagga cgaaagagtc gtaaatatgg agcgtaccaa
     1681 tatccgctat gtgacacctg aggacattgg cgaagaaatt gattttgcgt ctgttgacgt
     1741 gtcatttatt tcgcttaaac ttgtactacc cgttgcaaaa acacttcttg ccgataacgg
     1801 cgaaattgtt gcacttataa agccacaatt tgaagcagga cgtgaacagg tcggtaaaaa
     1861 aggtgttgta aaggacataa aggtacacta tgaagttata aagaaaattc ttgacttcgc
     1921 acgaagtatt gatttgcacg ttgcaggtct ttcattctca cctatcaaag gtccggaggg
     1981 caacattgaa tatcttgcat atttgaagaa agccgaatgt gaggataaca tcacagatga
     2041 aaaaatacaa gaaatagtag acaaatcaca caatctgctt taagaaagga atgaacagaa
     2101 atgcttgccg aaaatacagt tttaacaggc agacgtgcga taacacgttc atatgcaaaa
     2161 ataaatctta cacttgacgt actttcaaaa cgcgaaaacg gttatcacga cattaaaatg
     2221 attatgcaga cagtatcgct gtttgacctt attttagtcg ataaaacgca caggggtatt
     2281 tctatttcaa caaatttgcc gtatttgcct tgcaatgaaa aaaacatcgc ctataaagca
     2341 gcagacgcat tttttaaagc gacaggtatt cgcggcggtg caaagattgt aattcacaaa
     2401 aatatacccg tagccgcagg gcttgccggc ggcagcggta attgtgcggc ggttcttacc
     2461 gcaatgaata tgctccacgg caatcctctt tctaatgatg agcttatgca tatcggtgcg
     2521 tcactcggtg cagatgtgcc gtactgtttt gacggcggta cacagcttgc ggagggtatc
     2581 ggtgaaattt taaaaccgct ctctcctatg ccgaatgcgt acatcttact tgtaaaaccg
     2641 cctatcaacg tatctacggc acttatttac gaacaaatcg acaacgcacc tatcgaaatt
     2701 cgtcccgaca cagacggaat gattgactct ttaaataata acgaccttta cggcattgca
     2761 gacaggcttt gcaatgttat ggaaaacgtg accgctaaaa tgtatccgan nnnnnnnnng
     2821 ttaatgccat tatgagcggt tcgggtccga ctgttttcgg tatttttgac gattttgaaa
     2881 aggcaaaaaa atccgctgac agttttgctt atcagtttaa ggacgtattt cttacgcaag
     2941 ttttaaatta aacgaggtga ttatttttgg agaaagacaa ggatattata gaagtaatca
     3001 tcgacgaaca accgacaaac aataaaaaaa tcaagtttgc atattgggat aatctcgcaa
     3061 aggatttatc accgctgttt ataaaagtat catcggcagt atgtattatg attgttttgg
     3121 gagtattttt tgt
//
LOCUS       DAWACS010000139         3128 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3315_length_3128_cov_2.841198, whole genome shotgun sequence.
ACCESSION   DAWACS010000139 DAWACS010000000
VERSION     DAWACS010000139.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3315_length_3128_cov_2.841198"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          816 a          759 c          542 g         1011 t
ORIGIN      
        1 ttccgacaca cccggttcaa attcaatctt aacatgaccg tactgaccgt gaccgcccga
       61 ttgtttcttg tgcttgcctt caacggtagc cttgcccttg attgtttcgc gatacggaac
      121 aatagggtct tccaaaactg cgccgacacc gtattttgat tttaatttgc ttataataac
      181 atcaatatgc tgttcgccct gaccgtttat aagagtctgt tttgtttcgg tattgttcgt
      241 aattgtgaat gtcggatcct cctccataag cttgttaaga ccgcttataa tcttttcttc
      301 atcgcccttt gtctgcggtt taatcgccat agacaaaaca ggctgcggaa attcaatacc
      361 ggtaagtatg atattcttac ccttttcaca taatgtatca cctgtttttg tgctttcaag
      421 ttttgcaacc gcgccgatgt caccggcttt tatcgacttt gcgtcaagct gttttttacc
      481 gcaaagatta aataccttgc ctatcttttc gtttacgtcc ttgttcggat tgtaaagcgt
      541 gctgtcaccc tttacctcac cggaataaac tctgaacaac gacatcttac caacataagg
      601 gtctgcgatt gttttaaata caaccgccgc cgttgtttcc gtttcactct gtacaagctc
      661 aacaggctca cctgtatcgg caactctcgc tatttcctca atttccgacg gagcaggcaa
      721 atacttaccc ataccgtcca taagcgaacg cacaccgata ttatcctgac cgctgccaca
      781 gtatacagga taaataacac cgtccttaac accttttctt atagcttgct taatttcgtc
      841 aaaagtaaat tcttcaccgt tgaaatactt aaccataagt gcctcatcgg tttcggcaac
      901 agcctccata atcatttctc taagcggtgc gacaatttcc gccataccgt cgggtacagg
      961 aatatccact ctgtcctgtc cttcgtatct tcttgccgtc atatcgacaa tatcgacaaa
     1021 gcctttgaat tcgtcaccct ctcttatcgg ataaacgaac ggagttactg attttccgaa
     1081 cttttctttc atttcctcaa gagttttttg ataatccgct ctgtcatcat caattttgtt
     1141 gacaaagaac ataatcggca cacctctttg ttttgcgtat ctgaacacgt tctccgtacc
     1201 gacgcttaca ccgcttcttc cgctcaatac aataagtgcc gaatccgccg cacggatacc
     1261 ctctttaacg tcaccgacaa aatcaaaaaa gcccggtgtg tctatcatat tatatttcat
     1321 atctttccac tctaccgttg caagagcggt tgaaatactg aattgtcttt tgatttcttc
     1381 accgtcatag tccgttacag tgttgccgtc agcgactttg cccattctgt cggtcatttt
     1441 cgcattgaac agcattgcct ctgtaagcgt tgtcttaccg catttaccgt gtcccataac
     1501 ggcgatgttc cgaattgcgt ccattgaata cgttttcatt tgattgaccc tcctaatatt
     1561 ttttattttt cgctcatttt atttggctac taatcaattt gtatgtcctt tgctatagcc
     1621 tcgtatatta catatatacc acattatcat ataaagataa caaaaaaagt accgtcagat
     1681 tataaaaatc aaacgatact tttttgtaca tattcactaa cttttatttt gttatttcaa
     1741 cagttcttgt gtcaccgttc caatcaacag taacattaaa tgcttccatg acaaatctaa
     1801 gcggaaccat tgttctgttg ttcataagcg ttgcgggtgc atcaagcgtt atcggtgtat
     1861 catttatgta cgcaacatca ctgtcaagat acatcacgac tttgtcgccg ccctttttaa
     1921 ccgtaatctc ctgcttgtca ccgtcccaat ctacttttgc attgagagcc tctgatatac
     1981 ctcttacggg tatcattgta tacgtttcac cgtcaatggt ttcaagcaac ggatactggt
     2041 cgtcaaacgc aagcaattta ccgtctattc ttacctttgc acctatgtaa tcaatatcat
     2101 tatgataata tctcataacg tgttctatgt actgcgcgtc accgtaagta ttgtacggcc
     2161 aatttgtcgc atactgcgcg gcattgacgc gttccgaaag ccaatcgtca cccttcgccg
     2221 caattattct gtcaagcacg gtttgtccga agttataaga ctgaatcatt ttcatataat
     2281 cacaatcata tctgataagc gactgtgaaa ttaaatatgc cgcaaacggc actgccttag
     2341 tcggatcaag tctgtcttga agcgtccatt tttctcccgt agtgtcctca ccgaacttgg
     2401 cgaatgattt ttccaaagta ttttcaatct gcattatacc ccatgccgga tattcacttc
     2461 cgtcatcacg gtaactcaaa tttcttcccg atgattcttg catacatatt gccgcaagca
     2521 agtttgggtc tacaccgtat tggtcacctg cctgctcgaa taaatcataa taacttttta
     2581 ccgccgaatt aagctgagcc gtaccgattt caataccgtc catacttgtg ccttgatggt
     2641 cgggtatagc ccccgccgca agtgccgtaa atgacgatgc cgaaatcacc gccgcaagtg
     2701 ccattccaat aaatttcttc ataatcaact ctccttttat gataacttga tatgtacacc
     2761 cccattatac acttatcata ttaatccgtc aatggctgtt atttcctacg aatttcatat
     2821 tatatgtgtt cgttttgaat aatacagtga ttatttggta atactctatt atgaactaaa
     2881 tcaaaggagg atatatctat gaaacagtac attgaaatag tcgacatttt cggacgtgaa
     2941 gttatggatt cacgcggtaa tccgacagtc gaagttgagg tgcatacaga atgtgcgtcg
     3001 ggcagagcaa tcgttccgtc aggtgcgtca acaggtgttt ttgaggctgt ggaacttagg
     3061 gataatgaca aaaagcgtta cggcggtaaa ggcgtaaaaa atgctgtggc aaatgtaaat
     3121 gaaaaaat
//
LOCUS       DAWACS010000140         3094 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3354_length_3094_cov_2.422836, whole genome shotgun sequence.
ACCESSION   DAWACS010000140 DAWACS010000000
VERSION     DAWACS010000140.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3354_length_3094_cov_2.422836"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2099..2108
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          863 a          691 c          500 g         1030 t
ORIGIN      
        1 catcatctgc agtccgacta ctaaagttgc tttttccttg gcaagttcaa gtgcatagcc
       61 gtcaagttta aagctcgcct gaaaatcttt tgatatttcg tgtcttgacg gtgcatcacc
      121 catacgggtc attattcttt cgacatcgtg acttgttatt atattcaaaa gcgaataata
      181 agcaggtgca gggtaatttt ctttaatgct cataagacgt gcgtcaaatt cttcaacgtc
      241 aattcttccg cttaccgcgt caatcaacgc tctgcgcatt gggtaattca taacggagtc
      301 aagctcctta cccaagaaat attcacgtct ttcaccgtaa gcggacttat ttgacgcgtc
      361 ttcccaaact tcacctataa taactgcatc tttatcggct ttctttacgt tttctctaag
      421 ttctttaacg aaaaatcccg gtaattcatc gacaacatca agtcgccaac cggacgcacc
      481 gttctttatc catcttttaa cgattgcatc tttgtccgaa agcaaatact tacgacattc
      541 ttcactgtgt tcttcaatct gcggaagtgt cgtcattccc caccatgatt catacacatc
      601 aggccaatcc ataaatctaa accaagtgta atacggtgat tccttcgact gataagcacc
      661 aatgctgtcg tattcgccgt atttattaaa ataacggctg ttacttcccg tatggttaaa
      721 tacaccgtca agaattatgc gtattcccat ttcttttgct tttgagcaaa gctctttaaa
      781 tgtttcctca tcaccaaaca tcggatcgat tgtttcataa tcgcccgtat catatttatg
      841 gtttgagtat gctttgaata tcggatttaa gtaaattacc gatattccca aatcttttaa
      901 atacggcaat tttttaatta cacctttcaa attaccgccg aaaaagtcat ttgctttata
      961 ctcaccgcca aattgttcgg ctttataaaa cggttgttcg ccccaattac gctttattat
     1021 atctgtacga tttcctaaaa attctccgtt ttcgttgccg ttgcaaaatc tgtctacaaa
     1081 aatttgatac gcaataccag ttttaaacca atccggagtt ttgtaatctt cgttataaac
     1141 agttatctga tacgaattac tcggtgaatt aaaacacatt tcaccaattc cgccgagttg
     1201 ttttgaattg ttgccgtaat aaaccatacc tctttcggtt tcaagttcaa aataatacca
     1261 aacaagtcct gctttttcag gcattttcac gttcaccgaa tatatgcagt ggtcaccgac
     1321 atcaaaaata tacggcatat cctttcggac ttcgcccttg ccatcctcca tatagacaag
     1381 acgaaccgcc gacggaatac ctgcgcccga cacagccaaa cgcaatctga cttcaccgcc
     1441 gcaagtcacc gctccgaaag gaaatctata aaattttttt ctggaattat gctctatatc
     1501 catatttacc cctttatcgt aaaagaagtg acctaagtca cttctttatt tttattattt
     1561 aattggtgtc aagtgccaaa tgttcttatt gtaatcttca atagttctgt cacttgagaa
     1621 gaatcctgca cttgcagtat tcaagattgc cttttcaatc cacttatctc tgtcttgata
     1681 atcagcagac atcttttctt gagtcttcat atattcttca aaatctctga ttaccatata
     1741 tgtgtcaggg aatccgtagt caccgaacaa caatgtttga tacaaatctc tgaacatttg
     1801 gttgctgccc ggaacgattg aaccgtcaac aagctgttca agtgcgtgtc ttaggcttgc
     1861 gtttgatgag tagatattct taacttcatc agttcccgca tacttaactc ttgcggcaac
     1921 ttcgtcagcc tttagaccga agatatatat attgtcttca ccgacctgtt caagcatttc
     1981 aacgtttgcg ccgtcaagtg taccgattgt aagtgcaccg ttaagcataa acttcatgtt
     2041 acctgtaccc gatgcctcaa gacctgctgt tgatgtctgc tcagaaaggt cagcagcann
     2101 nnnnnnnntt tttcagcgat tgatacgctg taattttcag ggaatacaac tttaatctta
     2161 tccttaactc tcggatcgtt gttaatcata tctccgactg agttaataag cttgataata
     2221 agctttgctc ttttataacc cggagcagcc tttgcaccga atatatatgt ctttggatag
     2281 taattttcag catatacgtt atcttcaaga attctgttgt attctgcaag aatatgaagt
     2341 acgttcatca tctgacgctt atattcgtgc aatctcttac actgtacgtc aaagatagaa
     2401 tcaggattta caactatatc gttgtgttcc ttaatatatt gtgcaagtct aaccttgtta
     2461 ttgcgcttaa tttcagcaaa ttgcttcttg aatgcagggt ctttagcata tttctttagc
     2521 tttgaaagtt ctgaagcgtc cttgatccaa ttcttaccta tctttgaaga aataagctca
     2581 gtaagttccg ggttacagtt agcaatccat cttctgaatg taataccgtt tgtgattgca
     2641 tagaagtggt cgttattcat attatagtaa tcagcaaaaa tatcctgctt tagaatatct
     2701 gtatgcaact ttgaaacacc gtttactgcg aaacagcttg caagacacat attagccata
     2761 aatatctgat tatcagaaat aattgccata tacttatgct tagccgaatc gttaggataa
     2821 acttcgttaa gtctgatcat aagacgtctg ttcatttctt caacgatcat atatacacgc
     2881 ggaagaatct ttctgaacat atcaagcggc cacttttcaa gagcctcgct cattactgta
     2941 tggtttgtgt atgcgaatac atgagttacg atgtcccatg cctcatccca accaagacct
     3001 ttttcgtcca tcaacaaacg catcatttca ggaatagcca ttgtagggtg tgtatcattg
     3061 atatgaataa cgaccttatc aggcaatttt gacc
//
LOCUS       DAWACS010000141         3073 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3386_length_3073_cov_1.999669, whole genome shotgun sequence.
ACCESSION   DAWACS010000141 DAWACS010000000
VERSION     DAWACS010000141.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3386_length_3073_cov_1.999669"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    528..550
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    803..812
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1547..1556
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          940 a          506 c          739 g          845 t
ORIGIN      
        1 gaacaatatg ggtgtcgaaa atacggtact tggctttata tccggtttta cgggcggtgc
       61 gattgaaaat atacttgccg atatgaattg taaaaccgat tttataaagc ttgacaacgg
      121 tttttcgagg attaacgtga aaataaaatc agacgccgaa acagaaataa acggtcaggg
      181 tcctgatatt tcagacggtg cggtaaagaa actttacgaa aaacttgaca gtttgaatga
      241 cggcgatacg ttggtacttg ccggaagtat tccgtcatcg ctttccgatt cgatttactg
      301 cgaaattatg gactacctta aagataaaaa attaaatata gtcgttgacg caacaaagga
      361 cttgcttgtg aatgttctta agtataaacc gtttttggta aaacctaata atcacgaaat
      421 aggtgaaata ttcggtgttg aacttaagac gagaagtgaa gttgttccgt atgcaaagaa
      481 aatgcaggaa atgggtgcga agaatgtact tgtttcaatg gcaggcgnnn nnnnnnnnnn
      541 nnnnnnnnnn agggtgcggt gttcgttggt gaaaacggag aagtgtacga aagtgaggca
      601 cctaaaggca aattagtgaa ctcaacggga gcaggcgatt caatggttgc cggtttcctt
      661 gcgggatata tggaaaagca ggattttatg tatgcactta agatgggatt atcggcaggc
      721 agtgcaagtg cattttcgga aaatcttgcg accaaagaag aaattttaac tgtatttaaa
      781 acaatatgag gaggtaaaaa ttnnnnnnnn nntcaaaaga gagtatcaga cttgacggag
      841 tttcaagcac aaaaaaagaa accattcgtg caatggcaga ccttatggaa aagagcggaa
      901 aaatatcaga taagaatgct tatgtagaag gtttgataaa aagagaggaa gaaagtacga
      961 cggcagtcgg cgagggtatc gcaattcctc attataaagg cagtgcggtg acaaaacccg
     1021 gacttgcggc aatggtaatc aaaaacggta ctgattttga ttcgatagac ggtgagccgg
     1081 taaagcttat atttgcaatc gccgcacctg atacaaaaga aaatgtacac cttgacgtat
     1141 tgagtaaact ttccgttttg cttatggacg aagatttttc acaaaaactt attaatgcaa
     1201 aatcggtaga ggaatttatt tcaattatag atgacgcgga aactgcaaaa gataatgctg
     1261 aatcagaaca ggttactaca tctgataaga aaatacttgc agtaactgga tgtccgacag
     1321 gtatagctca tacttatatg gctgctgagg gacttcaaaa agcggcagaa aaagcaggtt
     1381 gtgcgattaa agttgaaaca aggggttcgg gcggtgcaaa gaatgttctt accgaatctg
     1441 aaatcgcaga ggctgattgc ataatcgttg cgggagatac aaaagttcct atggaacgtt
     1501 ttaacggtaa aaaggtaatc gttacacagg taagtgacgg tatttcnnnn nnnnnngtat
     1561 ttcaaaggct gatgaactta tcgcaaaggc acttggcgga aatgttccga tatttacatc
     1621 aaaggaaagt gatgagggtg tttcggctaa gtcggataat aaagagggca tcggtcacag
     1681 aatttataag caccttatga gcggtgtttc gcatatgttg ccgttcgtta taggcggcgg
     1741 tattctaatt gctatcgcat tcttgcttga cgactttact attgatccgt caaacttcgg
     1801 tatgaataca ccgattgcgg cattctttaa gacaatcggc ggtgtcgcat tcggtttgat
     1861 gttgcctgtt cttgcaggat ttatcgcata ctctattgcg gacagaccgg gtcttgcagt
     1921 cggttttgta ggcggtatgc ttgcctcaac gggtaacagt atattagaat ttacaaacag
     1981 ctactcaggc ggaattaacg gctatcttgc aaacacagta ttcaattcat caggtacaac
     2041 tgtttcggga tttttgggag cacttgttgc cggttttgtt gcaggatata ttgtactatt
     2101 gctaagaaag atattctcaa aacttcctga aagtcttgag ggaattaaac cgacattgtt
     2161 gtatcctgta cttggcgtat tcattatggg tgcattgatg ttgttcgtat tcaatccgat
     2221 tatcggtgtt ataaacgacg cacttgcaaa cctactaaac agtatgggtt caacatcaaa
     2281 ggttattctt ggtattgttc tcggaggtat gatggctatt gatatgggcg gtcctataaa
     2341 taaggcggca tatgtattcg gtacaatgtc aatcgcaaac ggcaactatg atataatggc
     2401 ggctgttatg gttggcggta tggtaccacc tatcggtatt gcacttgcaa caacattctt
     2461 taaaaatcgt ttttcaaaat cggaacgtca gtcgggtatt acaaactatg ttatgggctt
     2521 ggcattcatc acagagggtg caattccgtt tgcggcagcc gatccgctgc acgtaatacc
     2581 ggcaacagca gtcggtgcgg cagtagcagg tgcattgtca atggtattcg gatgtacatt
     2641 gatggctcct cacggcggta tattcgtatt ccctgttgta ggtaactggc ctatgtactt
     2701 ggtagcatta gcggcaggtt cggttattac aatgtttatg cttgcacttt taaagaaacc
     2761 tgtatacaaa gaaaattaat atataaagga gataagtatt atgaaagaat ttaactacac
     2821 aatcacagat ccacaaggta ttcacgctcg tcctgcgggt gtgcttgtaa aagaggcggc
     2881 aaaatatcag tcaacaatca ctcttgaaaa agacggtaag acaggtgacg caaagagaat
     2941 atttgctgta atgggcttgg cggcaaagtg cggtaataca cttaacgtaa aagttgaagg
     3001 ctcggacgag gacgaagctg caaaggcttt ggaagaattt tttaaggcaa atctataata
     3061 ttaaaaatgg tgt
//
LOCUS       DAWACS010000142         3054 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3403_length_3054_cov_2.679560, whole genome shotgun sequence.
ACCESSION   DAWACS010000142 DAWACS010000000
VERSION     DAWACS010000142.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3403_length_3054_cov_2.679560"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1074 a          516 c          678 g          786 t
ORIGIN      
        1 acggagcatt gcgtgaaact gacaacacac aaataaaaat cacaccgata atattatctc
       61 ttattattat aatcccctac aatatacaag tcaaatgtta cctttaacaa aaaattaata
      121 aaaaagatat tgattttttt atgctaaagt ggtatattgt taaagatgat gtaaaatgag
      181 gaatgatcat tttatattgg tacaataaat gaaaggagaa aatacagtta tgaacggagc
      241 cgaaaagaaa aaagcaactc ctgccgataa agcggtaaat gcgattatag ttttaattat
      301 attggtattt gtcgctgtag gagtttacgc aacatacggt aaaatatcgg aaggtgttac
      361 caacaaggag attgaaagcg gtaaagcgga ggcaactgtt gaatatcttg caaatcaagc
      421 cggtcaatct gttgaagatt accttgcaca gtacggtctt actcttggcg atacaatcaa
      481 agcaaagact actgaaagcg aaatgcttga caatatgact attgaaaatt atgctaaata
      541 caatggtcaa gaggctgaca gctttctgca aggaatgagc gattcagtaa caaaagatac
      601 tgtatggaaa gacttaatgg caatgcctgt atctcaagtt atagacgcag acgctcttgc
      661 acaggttaag aagcagtatc aaataggtga tgatgaaatt tcagattcaa ctacttacga
      721 agaattccaa aaagtgcttt atcaaaaggc atcagccgca tcacaagccg cacaggcaac
      781 tgatgcaccg acagcagaaa caacagaaac acctgctgaa taatattata taaggagatt
      841 tagaacagtg aacgacgaaa agaatctaaa tgaagaaaat aacgaatcag taaacaatga
      901 agaacaaact cctgtagaaa cagaagaaac aactgccaaa caggcagaag aaacagccga
      961 aacaccggct gaaacagaag aaacaccggt tgaaactgaa gtatcggaaa cagccgaaac
     1021 agaggaaact ccggcggttg ccgaagaaaa tacagacgta gaagttgacg aggctgttgc
     1081 ggcggcacaa gctgcttttg aaaacgttca aaaggacgaa cagaaaaagg gcggcaaagg
     1141 cggacttatc gctataatca tagctgcggt aatcgttgtt gcaatagttg tcgtacttgc
     1201 agttaagttc ggcccgtcaa tgttcaataa gtataacaga cttggttatg ttgacgtaag
     1261 cggacgtaca gttgcacaag ttgcagaaga acagggaatg gacgttgacg aattccttga
     1321 acagtttgat ttaccgtcag atatgcctgg taacacaaca gaatcagctg cttataactg
     1381 tattcctgta tcaaaaatgg cagaaatgta cggtatggac tttaatacaa tcaaagaagt
     1441 gctaaagttc cctgatgatg tagatgaaga tacagcttgg ggcgatgcca tcggtcaagt
     1501 aacacttggc gattatgtcg gtgaagataa tcttgctgaa ttcaaagaaa cttacggttt
     1561 tggtgatgat ataacagcag atacactttg gaaagacgta agacaaactg ttgacgagaa
     1621 gagcagacaa cagagaattg aatctgaaaa agctgaaaaa gaatcagata agaacaagga
     1681 tactgacagt gccgaatcaa cagaagcacc tgccgaaaca gaaagcgcag atgcaacaga
     1741 agctcctaat gcttaataaa ttttgaacca aggcggattg ctcaaagcaa tccgctttta
     1801 gaatgccgat aaattgtccg gaacgtgcaa aatattattt tttattctgt aatttattat
     1861 tataataaat tacaggcaag cctatctccg atatacggct tgcttgtgca ctttttgcga
     1921 aaataaactc taacgtacca tcgtacgccg acgagttcat tttcgcaaaa tccgaactaa
     1981 tttttcggct tctgccatcg tgtagacaaa aatgtccaca cgctgaagcg gattgcttaa
     2041 gcaatccgct ttttatatat tgaaactttt gaaaaatagt gatataattt atctatattg
     2101 tttttaagga gaagaatatg atagcgatta tagattacgg tgcgggaaat ctgcacagtg
     2161 ttaaaaatgc acttgatttc ttgaacgcag aaagcattgt aacaggtgac agtgaaacga
     2221 ttttaaatgc tgacaaggtg atacttccgg gtgtcggtgc gtttggtgat gcaatgaaat
     2281 gtcttgaaaa aagcggactt gttgaaacgg ttaaagctgt tgcaaagagc ggtaaaccgc
     2341 ttttgggaat atgtttggga cttcatttga tgtttgaaga aagtgatgaa acgccgggtg
     2401 tgaaaggtct tggaatattc aaaggcaaaa ttgtaaagat acccgaaaaa gacgggctta
     2461 aaattccgca tatgggctgg aacagcataa ctgttaataa gaatagcaga atattaaaga
     2521 acatcggcag tgagccttat gtatattttg ttcactcata ttatgttaat gcggaggacg
     2581 agagcattat ttccgcatac accgagtacg gtcaaaagct tgacatagcc gttgaaaaag
     2641 ataatgtgtt tgctacacag ttccaccctg aaaagagcgg tgaaacaggt atgactatac
     2701 taaagaattt tattgcatta taataaggag attacgatat gtacgcaaaa agaattatac
     2761 cgtgccttga cgttaaagag ggaagagtgg ttaaaggtgt caattttgta aatcttattg
     2821 acgccggtga tcctgtcgaa tgtgcaaagg tttatgataa agccggtgcg gacgagcttg
     2881 ttttcttaga tatcactgca tcctccgatc agagaaatat cgtagtggat atggtcagac
     2941 gtgtggctga gacgatcttt attccattta cggttggcgg tggtatccgt accgttgatg
     3001 atttcaaagc cattccccgc gaaggtgcag ataaggcatc agttaattca gcag
//
LOCUS       DAWACS010000143         3027 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3434_length_3027_cov_2.022544, whole genome shotgun sequence.
ACCESSION   DAWACS010000143 DAWACS010000000
VERSION     DAWACS010000143.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3434_length_3027_cov_2.022544"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    346..355
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1770..1779
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          969 a          482 c          744 g          812 t
ORIGIN      
        1 gtttgcgggt aagtgctatg acataatgaa aaagtttgcg gctaataata gttatatgaa
       61 ctgtcagata gtgctttgtc ccgaaattaa tgacggcaag gaacttgaca ggtcaatatc
      121 cgagctggcg gcactgttcc cgaatgtaaa cagtgtatca atcgttcctg tcggtcttac
      181 acgctatcgt gacggacttt atccgctgaa accgtttaca aaggaaactt ccgcacaaac
      241 aataaagcaa gttgagactt ggcagaaaaa actgtatgat aaacttggaa caaggctaat
      301 ttatctttct gacgaatttt atttgaatgc ggaattgcct ataccnnnnn nnnnnacgca
      361 gaagtgtatg agggttttcc gcagcttgaa aacggtgtag gattgattgc cagtatggca
      421 gaagaatttg attcggcaat taaacttgtg aaaaacaaaa agtatgacag aaatgtaact
      481 cttgtaacag gtgaggcggc ggcatctttt ataacgggtt tgtcggaacg tcttatggaa
      541 aagacagacg taaagataac tgtttatgcg ataaaaaata actttttcgg cggaggcgta
      601 aacgtttcgg gacttgtcac gggcggagat ataatcgacc agcttaaaga caagcctata
      661 ggggatataa tgcttattcc gcacagtatg ctgagagatg aagacgggat tttccttgat
      721 gatttgactg tatcagacgt tgaaaaagcg ttgaatgtga agatagagcc tgtcataaat
      781 gacggatatg aatttattga gaaaatcttg ggagaagaac ttgaatttta aaggagaaga
      841 atatgaaacc attggtagcg atagtcggca gaccgaacgt cggaaaatcg actttgttta
      901 ataagataac aggaaagaga ataagtatag tagaggatac accgggtgta acgcgtgaca
      961 gaatttattc tgacgcagtg tggcttgaca agcactttac gcttatcgat acaggcggta
     1021 tcgagcctgc gtcaaaggac gaaatccttg tacaaatgcg ccgtcaggca cagcttgcta
     1081 ttgaaatggc agacgttgtt gtgcttatga caaacgtaaa agacggtgtt acggcaaatg
     1141 accaggacgt tgcacaaatg cttttaaagg cgaagaaaaa gattgtgctt gccgtaaaca
     1201 aggttgacaa tacaggcgat ccgccgtttg aattttatga attttataat ttgggacttg
     1261 gcgatccgat tgctatttca tcaactcacg gtttgggtgt aggtgatttg cttgacgaag
     1321 ttactcactt gttccctgag gacgcagata cgtcagatga cgaggacgaa attaaagttg
     1381 cggtaatcgg taaaccgaat gcaggtaaat cgtcgcttat aaataaaatt ttgggtgagg
     1441 acagagttat tgtaagtaac atcgcaggaa caacgcgtga cgcaatcgac tcacattacg
     1501 aaaagaacgg cgacaagtat ctgtttatcg ataccgccgg tatgagaaaa cgcggtaaga
     1561 ttgacgaaaa cgtagagcgt tatagtgttg tacgttcgct tgcggcggtt gacagaagtg
     1621 acgtgtgtgt tattatgatt gacgcaaacg agggtattac agaacaggac acaaaggttg
     1681 ccggctatgc tcacgagcag ggtaaggctt gtatttttgc ggttaataag tgggatattg
     1741 tttctaaaga cacaaagact atgaaaaacn nnnnnnnnnt tatgtcttat gcggaaatta
     1801 tattcatcag tgcaaagaca ggtcagagag ttgacaagct tttggaactt atcaagaaag
     1861 taaatgagca gcacaagaga agaattacaa ccggtatgct taacgacgtc ttaaacgaag
     1921 caatggcaaa gcaacagcca ccgtcagaca agggcagacg tttaaaggtt tactacggta
     1981 cacaggctgc aacagcaccg ccgacattta ttttgttcgc aaattcaaag gatttgttcc
     2041 actattcgta tttgagattt atcgaaaatc aagtgcgtga ggcatttggt tttgagggaa
     2101 cacctataaa gtttatcgta agagagaaaa atgaaaagaa aatgtaatgg gagatagtta
     2161 tgacatattt aataattgcc ctgacggcaa tagtatcata tttaatcggt tcggttaatt
     2221 tttctatact gctttcaaaa atgctgagcg gtaaggatat ccgtgaaagc ggttcgggaa
     2281 atgcgggtgc aacgaatatg ctaagaacat acggcaagaa aatgggtgtt ataacactgt
     2341 tgcttgacgt gttaaaaggc atagtcgtga tacttttatc aagacttgta aaaaatattg
     2401 cgggtacaga tgaaatatat cctgttataa gctatatcgc aggtgtatgc gtaattctcg
     2461 gtcataattt tccgctttac ttcggattta aaggcggcaa aggcgttgcg acttcgctgg
     2521 gtgttgtgtt aatgcttgac tggaaagtcg gacttattgt ggctgtcgtt gcaatagcgg
     2581 ttatggcagt aacaaggtat gtatcgctcg gttcaatctt gggcggtgca atgtacatag
     2641 ttatagaaat cgttaaaatg attgttacaa agaacgttga tgcaattcag cttatgtgcg
     2701 ttgttattat cggcggactt ttaatagcaa gacatcatgc aaatataaag agattactta
     2761 gcggtacgga aaacaaactt ggagcaaaaa aaggagaagt aaaatgaaaa ttgcagtaat
     2821 tggctcaggc ggttggggaa ctgccatagc gatactttta tcttcaagag gacataatgt
     2881 atatttatgg tcgtggatac aggaagaaac ggacagactg aaaaaagaca gagaaaacaa
     2941 agaatttttg ccgggagtga aatttccgga tacaatttat tgcagtcacg atatggctaa
     3001 atgtacagac ggagcggagc ttattat
//
LOCUS       DAWACS010000144         3024 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3439_length_3024_cov_2.174470, whole genome shotgun sequence.
ACCESSION   DAWACS010000144 DAWACS010000000
VERSION     DAWACS010000144.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3439_length_3024_cov_2.174470"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1241..1250
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          980 a          477 c          701 g          856 t
ORIGIN      
        1 cccgaactta tcaatgacgg caaaacaggc atactttttg aaagcggaaa cttgaaagag
       61 ttgaccgaaa agatagaata tttgtataac aacaaagaac tttgcagaca aatgagtgaa
      121 aattgcagag atataaagta tgacacaata aaagaatatt ccgaaaaact gataaaactg
      181 tataaggaag ttataaaatg aaaatcgcaa tgataggaca taaaagaata ccgtcacgcg
      241 agggcggtgt tgaaatagtt gttgaagaac ttgccacaag aatggtcgaa aaaggtcata
      301 gcgtgacggt gtataaccgc aaaggaaaac acgtttccgg cggtaagacc gttaatttga
      361 aagaatataa gggggttaag ataaagagtg taccgacgtt tgaaaacggt aaactcaatg
      421 cgatagttta ttcgattata gcaacatttc tggcactttt cggcagatat gacgttatac
      481 attttcacgc cgaaggtcct tgttcgatga tatggcttcc gcatttattc gggatacata
      541 ccgttgcgac aatacacgga cttgattggc agagagccaa atggggcgga tttgccacga
      601 aatttctcaa attcggcgaa aaaacggcgg cgaaatatgc agatgaactt attgtactgt
      661 ctgagggaac gcgtgactat ttcaaagaaa cttacagcag agaaacggtt ttcataccga
      721 acggaataga tgttcaaaat gaaaagaaag ccgatataat cacaaaaaaa tacggtcttg
      781 aaaaagacgg atatatattg tttcttgcaa gaattgttcc ggaaaaagga ttgcattatt
      841 tgatagacgc atttatgcag acggatactg ataaaaaact tgtaattgcc ggaggagtaa
      901 gtcattcagg cgaatatgca aaacaaattc acgatatggc aaaagacgac agaataatat
      961 ttaccggctt tgtcgagggg gacgagcttt gggagcttta ttataattgc agaatgtatg
     1021 tacttccgag tgacgttgag ggtatgccga taagcctgct tgaagcaatg gcttgcggtt
     1081 gtgagtgcct tgtaagcgat atagatactg caaaaaacgt agtaggggag tatgggtata
     1141 catttaagaa aagtgatgta aatgacctta aaaacaagct gtgcgaaata ctcggcaaac
     1201 cgaaagcgga taaggcagag cagattgaat atgtaaaaaa nnnnnnnnnn tagtaataac
     1261 aaaaatattt aatataaaag gttttcactc tgcatttata caggattttt cgattacaac
     1321 aaaaatatct gtaactgaaa gagggaagat tttattaaaa aagcatatac atactaaacg
     1381 taatgttatt ttatgtgcag agggcggaac gcttgaaatc ggcgaagggt gttttttcaa
     1441 caacggctgt atggcggttg caaaagaaag aataaccata ggaaatcgag cggcattcgg
     1501 tccgaatgtg cttatatatg accacgacca tgacatatca tcagctgaaa gcatacacga
     1561 cagcggttat aaaacgtcgc ctgtcgttat aggtgatgat gtgtggatcg gtgcaaatac
     1621 cgttatactt cgcggaacgg taatagggcg tgactgcgtt gtcggtgcgg gttcggtgct
     1681 gaaaggcgta tatcccgcag gcagtgtgat agtacagaaa cgaactgaaa atatttatga
     1741 gaaagggcgt gtaagcggtt gattataaac ggtgaaaaac gaaagatagc aatggaaatt
     1801 gcgtatattt taatgcttat gtctgcggct atgggtgacc taaaagtgtt ttccgtttcc
     1861 caaacccgta tgatgaaagc tataagtctt ttcattgttg ttatggctgc ggcatacctg
     1921 tttatatcag gacggttgga aagagttaaa acggcggcaa gctttgtcgg tgtttacggc
     1981 tttgtcctta tcggaattat cgtatggtcg atatttttgt ggataatcaa cattgaatct
     2041 atcgacttca ttctgcgtgg cgcatcgaaa tttatgtatc agtttctggt gctgctcatt
     2101 attttctccg gtgcatattt gttcggagaa cgagcgatat ttactacatt ttacggactt
     2161 gcggcggcaa atatgctaat ggtcgtgtac aacttgggcg tatacggaat atcggacagt
     2221 ataaattccg taattgcaat gcttatgggc agtgatgcac aagaaggttt tgcaagggca
     2281 atggaaatac acgatattac gtttacatac ggctttttca taatatactt actgtttttt
     2341 gcacaacaca cgaaagagag aatattatgc cttttggtgt caatattctt cttcatactc
     2401 ggatggaaaa gaattgcact tttggctttg ccggttgcgt tgtttttcgg attgattatg
     2461 gggcgaatga aacctaacag acgtatcgga tttatgaaat tcatcggctg gtgtgcggtt
     2521 atcatttcgt ttgggtatgt ggttgtaaca aaaacaggtg catttgaata cataacgaat
     2581 tacttcggca tagatacaat gggtagaaat gatgtgtaca agtacattga aaaatattat
     2641 cagataagct taggttttat ggggtacgga tttgagtaca caacagtaat tttacagaaa
     2701 attatggttg acaatcctaa cgcacatatc ggtgtcgttg cacttcacaa taacatactt
     2761 acaatttaca tagagctcgg tttcttggga ttctgggcat ggatgatata tacttgggta
     2821 tttcaggtta attggatgat aaatcactgg ggagaaaaaa caggtatgct gttcttcctc
     2881 tgtgaaatgt acatctttat aacatacaca acagataata cattatacta tttctttaca
     2941 agtcttgtac tgagacttat gccgctggct tatgcgttcc atattccgac aacacaagat
     3001 ataaagctgt ggccatgggt aaag
//
LOCUS       DAWACS010000145         3023 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3440_length_3023_cov_2.421833, whole genome shotgun sequence.
ACCESSION   DAWACS010000145 DAWACS010000000
VERSION     DAWACS010000145.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3440_length_3023_cov_2.421833"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          974 a          604 c          592 g          853 t
ORIGIN      
        1 aaaatgcact caattagtat taatatattc aattttagca ttattgtggt aattacagta
       61 tatcctattt ttcttatttt gtaaaggtat caatttaaga tttgtaaaaa aacaaaaagc
      121 agagagtttt ttactctctg cttttctaag cttgtcgcgg tgacttttcg gaaattgctg
      181 ttacatttaa aaaggtgtgc cacctaagtg acacaccata aaatgcaaca ctcccggact
      241 gtcaccacaa caagtcaaaa gatatgctaa tttacttgca cacaaaatag agtgtagcat
      301 ttttaacaat aacaattaaa aatgtgcaca aataaaatta atattatctt ttgtcttatt
      361 gtggtgatta cagtatatct caaatcatta aatttgtaaa ggtggtttca ttttattttt
      421 tgcaaaatcg ctatcgtcag ctttaacaat aacctgtccc ttagcgtctg tcatcaaaga
      481 ctcacctgca aactccattt tgccctcgct tccaactctg ttgcacattg caaccgcaac
      541 acaattttga aacacttgtg cacatatttt cttttcaaat atatacatag actcttgctt
      601 tgtagtcact atcggtatta aaatcaagtc cacacccata agagtttctg ttatatccca
      661 aaaaacaaat actaatcttg tcaaaataaa cattgacaaa tatttaacac aggcgtataa
      721 tttattcttg gagagtagta tgacaatttt tagacattta attttacggc ttgggaatag
      781 aattgccatg taaaaaaaaa cggagggaga atttttatga attcaaatga ttgtttggaa
      841 aaacagccat tatgcgacga aatgcttaca aaaatggacg catggtggag agctgcgaac
      901 tatcttgcgg caggtcaact ttacttactt gacaaccctc ttttgagaga gcctcttaaa
      961 agagaacacg taaagcacaa aatagtaggc cactggggta cagtgccggg tcaaaacttt
     1021 gtttacactc acttaaacag agttattaag cgttacgacc ttgatatgat ttacatttca
     1081 ggtccgggac acggcggtaa ctttatggtg gcaaatacat atatggaagg ctcttattcg
     1141 gaagtatatc cgaacgtaag ccaagataca gagggtatga aaaaactgtt taagcagttc
     1201 tcattcccgg gcggtatttc aagccacgtt gcacccgaaa caccgggttc aatcaacgaa
     1261 ggcggtgagc ttggttactc acttgcacac gcattcggtt cggtattcga taatcccgat
     1321 cttatcactg cttgtgttgt cggtgacggc gaggccgaaa caggccctct tgcaacagca
     1381 tggcaatcaa acaaattcct aaacccaata aatgacggtg cggtattacc tatacttcac
     1441 ttaaacggat acaaaatttc aaacccaaca attttctcgc gtatatcaca cgaagaaatc
     1501 gaatcattct tcaagggatg tggttgggaa ccgtactttg ttgagggcga tgaccctatg
     1561 acaatgcaca aactaatggc agaaactatg gatacagtaa tcgaaaagat taaggcaatc
     1621 caaaagaatg cacgcgaaaa caatgacgcg tcacgtccaa agtggcctat gattgttctt
     1681 cgtacaccaa agggttggac aggtccaaag gttgttgacg gaaatcaaat cgaaggtaca
     1741 ttcagagcac accaagtacc tatggatatg tcaaagccgg aacatcttga ccaactaaaa
     1801 gaatggatgt taagctacaa acccgaagaa ttattcgacg aaaacggcag acttgttcct
     1861 gaaatcgccg cacttgctcc tgagggcaat cacagaatgg gcgctaaccc acacgcaaat
     1921 ggcggtttgc ttttaagaga tttacgtatg cctgatttca gagattatgc atttgacgta
     1981 ccgttccctg gttctgtcga gggacaggat atgattgaac ttggcggatt tgttcgtgac
     2041 atattcaagt taaacaagga aagcaaaaac ttccgtatat ttggtcctga cgaaactatg
     2101 tcaaacagac ttggcaaagt atttgaagaa acaaaccgtg actggaacgg tgaaaaatat
     2161 gataatgacg aatttcttgc aacagacggc agagttatgg actcaatgct ttcggaacat
     2221 atgtgtgaag gttggcttga gggctatatt ttgacaggtc gtcacggttt ctttgcaagt
     2281 tacgaagcat ttatcagaat aatcgattca atggctgcac agcatgctaa atggttaaag
     2341 gttacatcgg aacttccatg gcgtcaaaag attgcatcgc taaatcttat cttgtcatca
     2401 aacgtatggc agcaagacca taacggtttt acacaccagg acccgggatt ccttgaccat
     2461 atttcaaata agaaagcaga cgttgcaaga atttatcttc cgccggatgc caactgcttg
     2521 ctatcatgct ttgaccactg tatcagaagt aagaactatg taaacgttct tgtaacatca
     2581 aagcacccac gtccgcaatg gcttacaatg gaacaggctg ttaagcactg tacacaaggt
     2641 atcggtattt gggattgggc atcaaacgat aaaggttgtg aacctgatgt tgtacttgct
     2701 tgctgcggtg atacaccgac acttgaaatt cttgcggcag ctacaattct tcataagaat
     2761 atgcctgatt taaagattcg tgttgtaaac gtagttgacc ttatgaaact tcagccacag
     2821 tctgaacacc ctcacggtct tagcgacgtt gaatacgatt caatctttac aaaagataag
     2881 ccgattatat tcgcattcca cggttacaga aaacttgtac acgaacttac atataacaga
     2941 cataacagaa atcttcacgt tttcggttat atggaagagg gtacaatcac tactccgttc
     3001 gatacgcgtg ttcaaaacga aat
//
LOCUS       DAWACS010000146         3004 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3474_length_3004_cov_2.234995, whole genome shotgun sequence.
ACCESSION   DAWACS010000146 DAWACS010000000
VERSION     DAWACS010000146.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3474_length_3004_cov_2.234995"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    815..824
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2263..2277
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          821 a          662 c          483 g         1013 t
ORIGIN      
        1 tttcttcata ttcgacttca acttcataat ccgttttatc gaaatatgtc gttttatcaa
       61 ggcacaattc cgatccgtta tgcacaaggc tgtttcttat cgttacggca cagcccattt
      121 tataaagctc gcccacatcc tcgcctgtta tctcttttgc aagttcggca tttataatat
      181 caggtacttc ttccgtttca aattcatttt cttcgcaaat ctgcaaaggg ctgttttcgt
      241 ttttgtgaag tttcacctgt attttcgcca cttcacccac cactcttatt cttaccatta
      301 tacgtttttc tctaagtata cccgctttat cggtataata ataattcgtc tgttctctga
      361 ccttttccca tgtaaaagcg tttaaaatac tctcatacag gtctttgtct atcatacttt
      421 ttatctctgt ttccgtcatt tctgctcctt tatataaact gatttctttc ttcactgtat
      481 ttgtacccaa ttagtgacag tttatcctca atagtttttc tttcaagctc catatcgtca
      541 cacatatcgt caagatttga ataaaaatca cgaagttttg tgttgacaaa gctaagcaat
      601 atagcaggat cgttcggtat catattatcc ctccaagtga tctcttataa gtgtttttat
      661 ttcgtttttg cccttatgga tttcatattt cattgccggt ataaccgttt tggaatatct
      721 gatatttgtg ttcggtgatg attcgattat acagccctca ccgccggttt ctgtaaggac
      781 ttcacagaat tgaaaatcat tcctcgcttg tgcannnnnn nnnntgtcgg agttataacg
      841 gttttaacgt caatcccctt aaacggcgac cattctatga caactctgtc attttcgaca
      901 ttgtatgact tcaaatgtct gcgtaccaac accgcgccgt caacaacaaa cgtaagcata
      961 ctgtcgcaag ccgcctcatc aatgcttaca ttacttctca taacattaaa tccaaaatga
     1021 gcgctgtatg caaatttcaa atatttcggc acaatctgac cgcacgcaaa atcggtaatt
     1081 gttcctgccg gatatgcaat actcttgccg tttcgtcttg atacaagcat atctgcctct
     1141 tttataagtt taagttcgtc aagtttaggc atttcttcaa tatcgcaaga ccaaaattca
     1201 tggtcatcag acaacataag gaatatatat gctttaagtc cccaataagg tgagccgtgt
     1261 gcattataat gctctgccat aagaagattt gaataatcgt atccgacaga taaaattccg
     1321 cttgtatcga atatattttt tgaaagccaa ttttcaatgt ttcttattaa tattcctttc
     1381 ataacaccaa gcgaaaacgg tcttatatcc gccaaaatac aagcacccca aaacgccgct
     1441 tgtgcaaaac gatatgtgag tgaacgaccg taaggaagtg cctcgccgtc ctcgtcaaac
     1501 caatatatga acgtttttgc aaattccgtt gcacgttcct tgtatatatc ggaacgctcc
     1561 ttgtcgtcgt cctccattgc aacggcataa atcaatccgt aaaaatggaa tgcaaaacca
     1621 atgtaataat ccttttgtcc atgcagtccg tctatgtacc agccgttgcc aagatagaat
     1681 tcatctatcc ttgcaatatc tttgtccaac tgttcttgac tgtattttct gccgaccttt
     1741 ttcaaagcaa cgttgacaag cactctgaaa aatgtccagt tgctgtcgca aacttccttg
     1801 tcatttatag aataaagcca tttttcaaag ttgtttttcg cagtggattt aagcggctcc
     1861 catacctttt ccggtgcaaa aatcaaaccg tatgcaaatg cagccatttc aacaaaacgt
     1921 tggtctctat cgtggcaatc gccccagtaa ccgtcatttt ttttatccgc accgcttgtt
     1981 ataccggtta tatataactt ttcaaacgtt tcattttcac cgccgcctgc ccaaaacggc
     2041 acaagtcccc atagcggacg tgcaaaagcc tcaacgtccg ccgaaagatt ttcgtaccac
     2101 gcactgtgtc ttgcatactg tacctttccg ccgttgtaat acttttctat aggcgatatt
     2161 atatccgata actgttgtct gaaatattct tttgtataca taaagccacc tctgtcactt
     2221 tgaattatct taatattttt attatagcat ttatttacca aannnnnnnn nnnnnnntag
     2281 gttgacaatt aaacttttat aatatataat tatctcagtt tttaagttag aaaggaaatt
     2341 ttcaaatgaa gaagaaattt acaacaagag atttaatttt gtgtgcattg ttcactgcac
     2401 tttcggcaat cggagcgttt ataagaattc cggttccgct tgtgcctttt actttgcaaa
     2461 taacatttac aacattggca ggtcttttgc tcggttctaa aaagggcgca ataagcgttg
     2521 cggtatacgt gcttatggga cttatcggaa tacctgtgtt tacacaaggc ggaggtttca
     2581 gctatgtgct aaaaccaagt ttcggctttt tggtagcctt cattatagga gcattcgtaa
     2641 caggacttat tgtcgaaaag aaacaaaatc cgtcattctt gagaatactc ggtgcaagct
     2701 ttgccggtat ggcattcgta tacgttttgg gtgcgattta tttctatatt atatgtaact
     2761 ttgttattaa tgcacctgtc agtatcggta aaacagttat gtcgtgtttc gtgcttgtcg
     2821 ctccgggtaa tatcgccctt gcaatccttg cagcacttgt cgcaaaaaga attataccga
     2881 ttctaaatcg tcaaaaccag tcaactgttc cccaacctaa gactgtatga taaaaaaaca
     2941 ctgtgctttg tcacagtgtt ttttttgatt actcataata ttaaaaaaaa atacacccac
     3001 atca
//
LOCUS       DAWACS010000147         2995 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3486_length_2995_cov_2.315986, whole genome shotgun sequence.
ACCESSION   DAWACS010000147 DAWACS010000000
VERSION     DAWACS010000147.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3486_length_2995_cov_2.315986"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    865..874
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1514..1523
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          853 a          564 c          634 g          924 t
ORIGIN      
        1 aataaagttg tagataatat ccgaattgtg gcacttgccg atttgcattt aaaggaattt
       61 ggcaaggaca acgaaaaact tgtggctgag gttaaaaacc tcagtccgga tattgtcgca
      121 atagtcggtg atatgaattt ggaaagtcag ccggacaatt acagcagtgt aatatcgctg
      181 tgtaaacagc ttaatgaaat tgcaccagtg tattattgtc ttggtaatca cgaaattgac
      241 gcaatgcttt tcaaaaattc caatatatat aaggatatta aggcggaagg tattaagata
      301 ttcaataatg aaaccgaaac cgtcaatatc ggcggaactg ctattgatat aatcggtttg
      361 acgcaaaatc cgacggagtt tgatgaatac ggaaaagatt tctttgaaaa agcaatgagt
      421 gcggacgata atttcaaact tttgttgatg cattatccgg aatatttctt gggtaaatta
      481 aatgactatg atattgacct tgcaattaca ggtcacgcac acggcggtca agtcagactt
      541 ccgtttatag ggggacttta cgcggcggat caaggcttgt tccctaagct ttgtgacgga
      601 taccacgaag acggtaacag taagttcatc gtttcacgcg gtttgggtaa atcgggaatt
      661 gttccgagaa ttaataataa gcctgaaatc gtaattgcag atataagctg gtattaatga
      721 aggaagtggg gcgaatgaaa acaaaagagc ttaataaagg tgaaattatt ataacatcac
      781 aagatttgca taaaccgata tatgaatttt ttaaaagaat tcttgatgta atttgttcgg
      841 ttgtggctct gattgttctt tcccnnnnnn nnnncccctg cttttctggt aacggcattg
      901 gcggtaagaa gtgacggcgg acctgcgttt tataagcaga caagagtggg gaagaataat
      961 actcattttg agatgtataa attccgcagt atgtgtaaaa atgcagagaa tctgcaagat
     1021 gatttgatga aatataatga aatggacggt cctgtattta agattaaggg cgacccgaga
     1081 ataacaaagg tcggtaaatt tatcagaaaa tacagtatag acgaacttcc acagctttta
     1141 aatattgtta aaggcgatat gagcatagta ggaccgcgtc ctccgcttgt aagggaggtt
     1201 gaacagtata attcatatca aatgcagaga ttgcttgtta cgcccggttt gacgtgcttt
     1261 tggcaggcgt acggccgcag tgatctgtct tttgacgatt ggatggatat ggatatgaag
     1321 tacataaaac gtaggaatat tttccttgat attaaactta tcgttaaaac tgtttttgcg
     1381 gttattttta aacgcggtgc atattaaaga gcggtcttta tgtgtggtaa ttttgcgttg
     1441 tggtatgtga tttgttttcg taggggcgac caatggtcgc cctttttggt acattcaata
     1501 attggtgtac tttnnnnnnn nnngtcaaat acagaaatgt tttgaagatt acacaaaaaa
     1561 agaggcgacc aacggtcgcc tctacattgc cgaaaaaatt attcttcgcc ttcttcgata
     1621 gcgtctttat gtgaaagtga tattttacct gtctttttat caacttccat aaccttaacc
     1681 tttagccaat cgccttcctt aacaacgtcc tcaacctttt caaccctgtg attagcaagt
     1741 tttgaaatat ggcatagacc gtctttaccc ggaagaattt caacgaatgc accgaacggt
     1801 tgaattgtct taacaagacc gttatagatt tcgcctactt caggttcttt aacaatagct
     1861 tcaatagcgt cttttgcagc cttaccgctt tcttggtcaa ctgcgaagat atggattgta
     1921 ccgtcttctt ctatatcaat tttagcacct gtttctgcaa caatcttttg aataacctta
     1981 ccgccttgac cgattacttc acgaatttta tcaacgtcaa ttttcattgt ttcagctttc
     2041 ggagcatatg ccgaaagttc ttctctcgga gcagggatag cctttagaag tatttcgtca
     2101 atgatataac atcttgcttt tcttgtctta gcaagagcct cttcaattac ttcgtatggc
     2161 agaccgtcat tctttatatc aacctgaatt gatgtgatac cctttttagt accggctacc
     2221 ttaaagtcca tttcgccgta gaagtcctca agaccctgaa tatcaaccat tgttgtaaag
     2281 ccgtcttctg ttgttataag accgcaagag atacctgcaa caggggcttt aataggaacg
     2341 cctgctgcca taagtgcaag tgtagaaccg cagacactgc cttgtgaagt tgaaccgttt
     2401 gaagaaagaa cttctgatac aactctgata gcatatggga actcgtcctc tgacggaagt
     2461 acaggaacaa gtgaacgttc tgcaagtgca ccgtgaccga tttcacgtct gcccggacct
     2521 cttgaaggtc ttgtttcacc tactgagtat gacgggaagt tatagtgatg gatatatctc
     2581 tttgtttctt caaggtcgat accgtcaagc ttttgaattt ctgaaagagg agcaagtgta
     2641 gcaactgaaa gcacttgagt ttgtcctctt gtaaacatac cgctgccgtg tgttctcgga
     2701 agcaggtcaa cttctgccgc aagcggtctg atttcatcaa ggcctctgcc gtcaacacgt
     2761 ctgccctgaa caagccatgc gcgaacgatt tccttttgca ttttgtaaag aatttcgccg
     2821 atttgctcgt ttatgtcagg gaactgttct tcaagagcag ggataatttc gtctgtaatt
     2881 tcaccgattc tttcatcacg aacatttttg tcgtctgtat caagagcata ctgtaacttc
     2941 tcaaaagcca tattcttgat tgcgtcatat aggtcatggt caacatcgtg tgatt
//
LOCUS       DAWACS010000148         2991 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3491_length_2991_cov_2.561649, whole genome shotgun sequence.
ACCESSION   DAWACS010000148 DAWACS010000000
VERSION     DAWACS010000148.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3491_length_2991_cov_2.561649"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    352..361
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2453..2462
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          978 a          501 c          719 g          773 t
ORIGIN      
        1 ctgattacag agaagttgac aacgacgata acaatcaaca ataataatga aaacactaaa
       61 aggacggaca aatgtccgtc cttttaaggc ttttaacaaa gcctttgtat taacacttta
      121 caaaactaaa atattgtagt ataatagcat aggtaggtga aaaaattggc tgaaaaaaga
      181 gattattacg aagtgctcgg agtttcaaag ggtgcttctg atgatgaaat aaaaaaggca
      241 ttcagaaaga tgagtaagaa ataccatcct gaccttaatc cgaacaataa agaggcggag
      301 aaaaagttta aggaggtaaa cgaggcttat caagtgcttt ctgaccctga annnnnnnnn
      361 nggcggtggc tttaacggcg gcggctttga ctttggcgat atattcggcg atttcttcgg
      421 aggcggtttc ggaggattcg gcggcggtca aagaagaaac ggaccaaaac gcggtgctga
      481 tattcgtaag attattgatc ttacttttga agaggcggca ttcggctgtc acagagaaat
      541 gagtatacag gcacaggaaa aatgtgaaac ttgcggcggc agcggtgcta aaaagggtac
      601 gactgcacaa acttgtcagc attgtcacgg aacaggtcaa atccgtacac aacaaagaac
      661 tgtactgggt tatatgacta acgttacgac ttgtccgcac tgtcacggcg aaggtaagat
      721 tgtaaaagat ccttgtccgg actgtcgcgg tacaggtaag gtaagaaagt cgaagaagat
      781 agaagttgat attccggcag gtattgatga cggtcagaca attcaacttt cgggtaaagg
      841 cgaggccggc gataaaggcg gtcctaacgg cgacttactt cttacaataa gagtacatcc
      901 gcatcagatg tttaaacgtg agggaacgaa tgtatttata gatatgccta tatcatttgt
      961 acaggctgct ttgggtgcaa ccgtacaagt acctacactt gacggccctg ttgagctgaa
     1021 aatccctgag ggaactcaga caggttcaaa attcagaatg aagggcagag gtattccgtc
     1081 aatcagaagt aaggtaagag gcgaccaata cgttactgta aacgtagaag tgccgagaaa
     1141 ccttaattca aagcaaaaag aacttttgcg tgaatttgaa gaagataaaa attataaaca
     1201 aaagaaatct tttgcagaaa agatgaaaga tttcttgaac aaataaactt aggaggaaga
     1261 caatgaaccc gaatatttat gaactttcaa aaatgacaaa agagcagtat gatttcataa
     1321 tgaaacgtgc agaacttgat attacagaac aaatgaaagt tgccaaagaa gtttctgacg
     1381 atgtcaaggc acgtggtgac gaagcagtat tggaatatac tgcaaagttt gaccgtgtac
     1441 agcttactgc tgataaaatg aaagtaacac ctgaagagat agaggcaggc tacaataacc
     1501 ttgataagga aacaagagag gctattgaat atgcttataa gaacatatac gatttccatg
     1561 aaaagcaact tccggaggaa atgtggttta ctatggtcga tgacggactt atggtaggcg
     1621 aaaagactac tccgatagtt gacgtatgtc tgtatgttcc tcacggcaag ggttcattcc
     1681 catcagtttt gtgtatgctt ggtataccgg cagttgttgc gaaagttcct aagattgttg
     1741 ttgtaacacc tccgaatgaa aagggtgaag ttgacgacgc tattttggca gcggcaaaga
     1801 taatcggtat cactgaaata tataaagtcg gcggtattca agccgttgca gccgttgcat
     1861 acggtactga aacaattcct aagtgtcata aaataattgg accgggtaac agttatgcaa
     1921 ctgccgcaaa gagagttctt tcaaaccata ttgacgcagg tttgccggca ggtccgagtg
     1981 aaattattgt tcttgcagat gaaaaggctg accctgaaaa ggttgctctt gactggatga
     2041 ttgaggctga acacggccct gacagtgccg cacttcttgt tactcactca aaggaacttg
     2101 ttgaaaaggt tgttccgatt gtaaacagac agcttgaaaa gatttctccg aagagaaaag
     2161 aatttataga aacaaacctt acaacatacg gcggtgtaat tcttacaaat tctcttgacg
     2221 aatcaatcga ttttgtaaac gaatacgcac ctgaacatat ggaagttatg acagaaaagc
     2281 cttttgatac acttcctaag attaagaatg caggtgaaat tcttcttggc gattacacac
     2341 ctgtaacact ttgtaacttc gttttaggtc ctaacgcaat cttgccgaca ggcggttttg
     2401 caaagactta ttcatcagta agtgttcaag acttcttaaa gcgttcatca gtnnnnnnnn
     2461 nncgttttta aatcatatga ttgagcatat tgcatggcgc ggtgaggtca atattgatgt
     2521 tgacttaaag cttgatgaat ttgttttgac acacgttata tgtgaagatt taggtattgc
     2581 acttggtaag gcggcaaagg aatatatcga ccgtacagac ggtgcgagag gtttcggtga
     2641 cgcagtcggt attatagatg aggcaaaagc ggaatgtgcg ttgagctttg aatcaagagc
     2701 atattgcgat attgattatc acggtattga gcttagtgaa aaagttgagg gaatggatac
     2761 ggaggatttg gaaacattcc ttgaaggttt tgcacagggg gctatgtgta cgcttcatat
     2821 cgaccttgta aaaggtcata acggacatca tatttgggag gcaatatacc gtagtttcgg
     2881 cagtgctttg aataaggtat ttgaagtgtc tgaaagccgc aagggcaaaa caagcggtgt
     2941 agccggaaaa attgattggg taatagaata atattaggga cactaaatag g
//
LOCUS       DAWACS010000149         2973 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3517_length_2973_cov_2.887937, whole genome shotgun sequence.
ACCESSION   DAWACS010000149 DAWACS010000000
VERSION     DAWACS010000149.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3517_length_2973_cov_2.887937"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          974 a          493 c          698 g          808 t
ORIGIN      
        1 ggaaaagctg gagcggattt aaaatagtcg agggacgttc caaacgacag tatgtggatg
       61 aaaacaagat tgcagaggtc tgccgaaata acggttatac catgtcgcag atatataaaa
      121 gcatgcttat aggtataact gatatggaaa agcttatggg cagaaaaatt tttaaagaac
      181 tgctcggtga ctatatcata aaaccgaaag gcagactgac acttgtgccg gaaacggata
      241 agagagaaga agtacataca ttgggtgagt ttagaaagga attttaatat ggcacatttt
      301 gtaacgggta aagtcagatt gtcatatgaa catatatggg agcctgcatc tattaacgac
      361 agtgatgaaa agtacagtgt ttctttgatt ataccaaagt ctgatacaaa gacggtaaaa
      421 gatattcaaa acgcagtcga acaggcaaag caggacggca aagcaaagtt cggaggaaga
      481 atacctgcga atttgaaact gcctcttcgt gacggtgata ttgaacgtcc tgatgatgag
      541 gcatataaag actgctattt cataaactgc aattcaaaag ataaaccgca gattgttgat
      601 aagaatgtac agcctatttt agacagaaat gaggtttaca gcggatgtta tgcaagagtg
      661 tcaatatctc tttatccgtt taatacaaac ggaaataaag gaattgcgtg cggacttggc
      721 aatatacaga agattgcaga cggagaaccg cttggcggac gtaccagagc agaggacgat
      781 tttgaagcat atgacgagga agatgatttc ttaaattaag gtgaattgga gatgtatgca
      841 aaaaaagaac acctcgtaag atgttcttaa ataatatgct gttgaagcca ttgaagaata
      901 tcagaatcag actgagttcc tgacgcaact gatagaataa gttcaatcaa ttcggtatca
      961 gtgtacttta aatcaatgcc gtttattgca agaaatacaa gcattgcgtg agtgccgatt
     1021 cgcttattac catctacaaa cggatgattt tttacaagtc caaaacatag gcgagaggct
     1081 ttatccaata aagtcggata cagttcctct ccaccgtatg tttggaatgg tgtattgata
     1141 gctgattcta aaagagaatc atcgcgtata tccagtgaac ctccgaattc tttcacaagc
     1201 tgtgaatgta ataataaaat ttgttcttta gtaaggattt tcattttgca agttcctcat
     1261 atacggcctt attacgagta attaaatttt ctgatatagc taatacatca tcagtttgtg
     1321 ctgataattg ttcatcagct tgattgaatt ctaaaattag atagcgaggt gagttgtttt
     1381 ttagaattac agcagaaccg tactggtcaa caagtcttgc tactttagaa aagttttgat
     1441 tggcttcgga aatagaaacc attgttttgg tattgacatt catattgcat acctccattt
     1501 cttttatata aatattatat tcttatttta ggataaagtc aacctaaaat ttaatggtaa
     1561 atggtgaaaa ctatgaaaaa attaagctgt gatattgaaa ccttttctga cgtagacctt
     1621 ataaggtgcg gagtatataa gtatgcggac tctcctaatt ttgagatgtt actgtttgca
     1681 tatgctgttg atgacggtga tgttcatata attgacattg caggcggtga agaacttccg
     1741 gaggaaataa tacaggctat aaaatcagat accgttgtga aaacggcata taacgcacaa
     1801 tttgagcgtg tgtgtctgtc aaggtatctt aagcttcccg atggagaata tcttaatcct
     1861 cagtcttggt actgcacagc tgtgcaggct gcggaactgg cactgccttt gtcgcttgcc
     1921 gatgtcggaa gtgtactcgg attagagagg cagaagatga ctgagggcaa ggagttaatt
     1981 aagtatttct gtgtaccgtg caagccgaca aaatcaaacg gaaactgtac ccgaaacaga
     2041 ccgtgtcacg atataaacaa gtgggaaaca ttcaaaaaat actgtatgcg tgatgtagac
     2101 gttgaacgtc agattgccga taaacttaaa atgtatccta taagtgatga ggaacatcga
     2161 ctatatgttc tcgaccaaat aataaacgac agaggggtat tagttgacag tgaattggct
     2221 gagcaggcgg taaagcttaa ttcaatacag acggctgtgg cggttgaaca ggcatatatg
     2281 ataacgggac ttgaaaatcc taattcagta acgcagttaa aacagtggct caaggaaaag
     2341 ggtgtggaaa ttgaaagtct gtcaaaaaag tctgttaaaa gtctggcaga tgaaacagac
     2401 ggagatgtca gtgaaatgct gaaactccgt ctgcttatgg cgaaaacgtc cgtcaagaaa
     2461 tatgaggctg taatacgcag tgtgtgcagc gataaccgtg tacacggaat gatgcgtttc
     2521 tgcggtgcaa acagaacggg aagatggtcc ggtaacattc tgcagccgca aaacctgcct
     2581 cagaaccatc tgccggactt aacacttgca agggatattg taaaggacgg agattttgag
     2641 atgcttgaca tgatgttcgg aaacgtaccg aatgtcctat cggaacttat aagaaccgtt
     2701 ctcattccaa aaccaaacca tagatttatt gtcgcagact tttcggctat tgaggctcgt
     2761 gtgcttgctt ggatagcagg agagcagtgg cgaatagata cattcaaaaa cggcggtgat
     2821 atttactgtg cgtccgcaag taagatgttt aaagtgccgg tggagaaaca cggagtaaac
     2881 ggcgaactgc gtcaaaaggg gaaaatatcc gaacttgcct gcggttacgg cggctcagtc
     2941 ggtgcgttaa agaatatggg tgctgttgag atg
//
LOCUS       DAWACS010000150         2973 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3518_length_2973_cov_2.508568, whole genome shotgun sequence.
ACCESSION   DAWACS010000150 DAWACS010000000
VERSION     DAWACS010000150.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3518_length_2973_cov_2.508568"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    209..218
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    942..951
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          890 a          686 c          463 g          914 t
ORIGIN      
        1 atctcctttt gccattttta tcataagctc atatattttt tcaagtgcgt ctactttggc
       61 aagcggtttc aaattttccg acatttcttt caatctctcc ttatcgctta ccatagacat
      121 tattttatcg tataatgcgt cagacgtcaa atctttttct aaaattaatt ctgccgcatt
      181 cttattttca aattctcttg cattctgcnn nnnnnnnngc acaacattcg gtgacggaat
      241 aagtattgac ggtttaccga gtgcagcaat ttcgcttacg gttatcgcac ctgctcttga
      301 tacaacaacg tctgccgccg ccatacactc cgccatattg tcaatatatg acacaatctt
      361 tatgttatcc gaaagagtta ttctgctctc cttaatttta ttcattattt tatcgtaatt
      421 tctgtcaccg gttccgaaca gcaattcgat tttattgtcc tttgatattc tgtcaagcat
      481 accgattacc gtatcattaa ttctgtccgc acccaaactg ccgccgaaaa taagcacaaa
      541 cggctttttt atgccgagct tttctcttga ctttttgtaa tcggaatgaa gtatttcact
      601 tctgatcgga ttacctgtta caacacattt ttccttatgc tgcatatgat taattgtttc
      661 ttcaaagctg agtgcaagat atgtaacata atttgcactg ccttttaccg tcatacccgg
      721 atacacattt tgctcatgta taagcgacgg cactttcatt ttctttgatg ccatagcaac
      781 agggccgctt acatatccgc ccgtacaaac gatacaatcg ggtttaaatt cctttataat
      841 cttattgcac tttcttcttg cggaaataag tttttttacc gtttcaaaat ttctgaaaat
      901 atgctttctg tcaaaacctt gtatttcgat atattcaata tnnnnnnnnn naatggaaac
      961 cgcatctgca acagctcttg taagttcaat atcatatcct gctttcggta caagttttgt
     1021 ttcaagtccc gattttgtac cgacaaacaa agccttaaaa tccttatcct gtttagctgc
     1081 ataatcggcg attgaaatag ccggattaat atgtccgcct gttcctccgc ccgcaaataa
     1141 tactctcata aatcttcctc cacattaatc ttttacggaa tgtcttgata tattaagcaa
     1201 tactcccatt tcacataaaa gcgttattat tgccgtaccg ccgtaactga aaaacggcaa
     1261 tgcgacacct gtattcggta cgcttgacgt tgcaacggcg atattaagta tcgtctgaat
     1321 agcaagctga gccgcaatac ccaccgcaac aagtgagccg tatgtatcgg gtgcattcat
     1381 agcaattctt attgctctta taataagcgc cgcaaaaagc aaaattacaa cagccgcacc
     1441 gataagacca agttcctcac aaacaatcgc gaatataaag tcattatacg gttcaggcaa
     1501 atacgaatat ttctgtacac tctgtcccaa gccaagtccg aaaataccgc ccgagccaat
     1561 cgcataaagt ccttgcacaa cctgataact ttcgtcacga atatccgcaa acggattcat
     1621 aaaacttgtt acgcgtgccc atcttacacc gctgagtgca cggacgccga ctaagccgat
     1681 tggtaaaaga ataagcgctc cgataagtac gggtttaatc ggtgtaccgc ctgcaatcat
     1741 aacgcttaca ccgataccgg caataactat cgcaccgcta aggtgtgttt ccataagcat
     1801 aagtccgaca actataagca taactcctat atacggcaaa ttacccttaa gatgtttcaa
     1861 attatatttt cccgaatcga caagtcgtgc aaaatacatc gctataacgg gtttcataaa
     1921 ttccgacggt tgaagctgaa gaaacggagt attaagccat cttctcgaac cgttcaattt
     1981 tacgcccaaa cccggtataa gaacgcagac caaaagtatt gtacatacaa tcataaactt
     2041 cggaacggta tttttatatt tattgtaatc aatccttgaa ataacccaca ttccgataag
     2101 tcccaatatt gcacaaagga attgtttatt aaagaagtag tacgaattgt tatgtaaagt
     2161 atttccggca ggtgcagacg ccgagagcag cattaccaaa ccgaatgaaa gcaacaatat
     2221 tactataaac aaaaatgtat agtctatctc tcccattcgt gcaagcttaa ccggttttcc
     2281 tattttagca cgagttgttc gtctattctg ttttatagct cttttaggat atgcagtccc
     2341 tgccatatct aaatctcctt ccgattaatt cgtcttaaat tgcaaaatat gcaacagcac
     2401 aagtaattac cgttaccaat gtaaataccg caacgatttt cttttctttc caaccgcaca
     2461 tttcaaaatg gtggtgaata gggctcatct tgaatattct tcttccctca ccgtatttct
     2521 tctttgtata tttgaaatat gcagtctgca taattacaga aagtgtttcc ataaagtata
     2581 caaagcctat caatataaga tgtataggca tttttaggtc aatcgccata agtgcaaccg
     2641 acgcacccaa gaaaagtgag cctgtatcac ccataaacac ctttgcagga tatttattat
     2701 accacaaaaa tcctatcaaa ccgccgatta atgccgctga aaatacagtc acgccatact
     2761 ctttaaatac gatatatgac acaagcataa agaatattcc aactattgtc gttatacttg
     2821 atgccaaacc gtcaagtccg tctgtaagat ttacagcatt tacaacacct acgacaacga
     2881 acattacgaa cggtatgtaa agccatatcg gcattatcca ttcaatactt gtaaacggta
     2941 ttttaatcgc cgtatcaagc tcgcctatga agt
//
LOCUS       DAWACS010000151         2932 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3565_length_2932_cov_2.307960, whole genome shotgun sequence.
ACCESSION   DAWACS010000151 DAWACS010000000
VERSION     DAWACS010000151.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3565_length_2932_cov_2.307960"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    485..494
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2416..2425
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          899 a          611 c          465 g          937 t
ORIGIN      
        1 tcgtaaaaca gccacaattt tttaaacatt tcaaactgtg aattatattt ttcaagtaaa
       61 tattccattg cacccttaaa aacaccgctg ttgtagtacc gttcaaaaac ctcctctatc
      121 ccctttaaca aaagcacatc ttcataggaa ataaagtcgt ttttaagcac ttcatacggc
      181 ggttttgagt tatatcggta accgtatttt tcttcttcac ttcttatttt cgttccgcgt
      241 aaaagtttta ggaaaccaag ctgaagtaca tcgggacgaa gattaaacac atcgtcaaac
      301 gatttaccga acgaattaaa atcttcaaaa ggcaatccga atataaggtc aaggtgcata
      361 tgtacaccgc ctgctttttg cacgagtttt acggcacgtt ttatcatctc aaaatcggtt
      421 tttcggtcta ttgcctttat cgttttgtca tttgttgact gtacaccgat ttcaagctga
      481 aaaannnnnn nnnntttata aggtccgccg caacttcaaa atgaaaagtc gtatttttcg
      541 cattgtcgat taaaaacttc accaactcac aagtacgttt tctgtccgca ttaaacgttc
      601 tgtccacaaa tttaacaatt ctcacattat gtcgcacgaa gaacatcaat tcttctttca
      661 cgacgtccat atccctaaaa cgcacactat gcgttgttga cgaaaggcaa taactgcacc
      721 taaacggaca accgcggctt gattcgtagt atatcagctt gccgctgttt ttttcaatat
      781 cttcgtccgt atacggaaac ggaattgacg taatgtcgtg aataagctct ctgtctttgt
      841 ttcgtattat ctcgccgttt tcacgatagg taatcccgtc tgccatttca ccgatttcaa
      901 gccattcttt aaaggtcgct tccccctcgc cgcgcataat cgcgtcaata aaatcgtact
      961 tttgcatata ttccgtatcg tcaaaactga cctccggacc gccgaaaata atctttgttt
     1021 ccggcgaaac ctttttcagc atatcggcaa catcaagaca gacctcaaca ttccaaatat
     1081 agcacgaaaa caaaaccacg tccgcttttt tcaaaaaaat cgaacgcagt atgtcctcta
     1141 ctctctcatt aatcgtgtat tccgccatct caacattatc cgaatttgcg tacgctttaa
     1201 gcgttcgtac actaagcgaa gtatgaatat attttgcatt aactgcaaca agcagtgctt
     1261 tcatatcagc caccgtacac ttctctcttt agtataccga tgtatggcaa atttctgaat
     1321 ctttcgtcat agtccatacc gtagccgaca acaaattcgt caggaatttc aaaaccttcg
     1381 tatttaacgt caacctgtgt ttcacgtctt gcaggctttg aaagaagtgt acagatttca
     1441 acgtttgcac ctctcttttt tagaagcttt acaagtgcgc ttagcgtatt tccgctgtca
     1501 ataatatctt ccgcaataat tacattttta ccgtttacgt caatttgtcc gtctttcaaa
     1561 atcttcaatt caccggaaac agacgaactt ccgtagcttg aaacctgcat aaagtcaagc
     1621 gaaacgttta cgttaagata acgcataagg tcagccgaaa aaacaacact gccctttagg
     1681 ataatcataa gtacaatatc cttaccggca taatctttat ttatttgctc agccaaatat
     1741 tttactttct cttgaatttc gctttcatta atcaatattt cctgtacttc gtttatcatt
     1801 attaatattc ccctttttag tatgatattt ttattataac atatattttt gaatttttta
     1861 atagaaaatg cgaaaaaaaa acgaattttc ggaaataata tttttatgaa gtaactatta
     1921 gttaaaaaaa tcgagaaagg cgtggttttc tatgaagaaa atactgtttt ttataccaat
     1981 ccttacactt atgttcaata ctgtctttgc catgcccaca aaaacgaatg atgaaatcat
     2041 agcagattac ccttttacga acagagtaaa tgagctgtcc gtttcggaaa agcaggaaat
     2101 cacgaagatc cttaccgcct gtgctgatgt tatgcggttt aatataagaa attataacta
     2161 tgacaaactt ttcaaatatg tgctttacac tcacaaaaat tttcaaatcc ttactgacat
     2221 tccggctgat acgggaaaca gcagtaattt gggttataac aatgttacac ttgtgaacag
     2281 tgattatatc gattatatta tgacgagagt tttccgcata acacccgaaa aaccacctgt
     2341 aaatgagctt ttggaacgcg ttttctgtta caatgacggt tactattatt atatcggcgg
     2401 tttcggtgtt tatttnnnnn nnnnntcggt gacatttatt acgaaggtga cacaagaaca
     2461 cccgaataca gttttgcaat tctgcaaaac accgaaaacg gatattcatt aatgcgtctt
     2521 ggtatgggtg aagatttgcc gacaagcgat gaagttcgat tgtattcgcc gtttaaaact
     2581 tacaacgaaa tgaattggaa tataaataat gtggacaata ataatgatga tgattttgta
     2641 aaatcgtata tctcaatacc gatactcttg cttgtcatat cgcttggatt gataggactt
     2701 accgcgtgta tcgctgcact tgtaaaaaga aaatagagtg gaattgggaa gttacctcaa
     2761 gattccactc tcagacatcg acaaattgtc cggaacgtgc aaaatattat ttttataatt
     2821 taaattatag ctataacaaa taacaggcaa gcctatcttc gatatacggc ttgcctgtgc
     2881 actttttgtg aaaataaact ctaacgtaca aacgtacgcc gacgagttca tt
//
LOCUS       DAWACS010000152         2897 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3617_length_2897_cov_2.554539, whole genome shotgun sequence.
ACCESSION   DAWACS010000152 DAWACS010000000
VERSION     DAWACS010000152.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3617_length_2897_cov_2.554539"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          751 a          661 c          463 g         1022 t
ORIGIN      
        1 tcaagtaata gttccatctt tgctgcctta tctgccattt cacgataatt gtcaatagaa
       61 ataaccaacg ctgtcggctg attatttttc agtacaatat attctgcatt attatttttt
      121 acgtcttcaa atatctgtgc agtttttcct ttactaaaat cagaaatagg tataagttta
      181 tcggcaaaat taagaatttg attattaata gttcccatac taaaaacctc ctgacatata
      241 tagtattatt ctttctatga ttattatact caatccaagc caaaaagtca ataaatttac
      301 ctaaatattt ttatgttaat ttattttaaa atacaataat acccctactg tcataaacac
      361 ttccctgctg tccgccgtta cgaactgctc tgtcgagtgc cattaccgct gcaaccgcac
      421 cgtctattcg ttcggtactg tgtttcttag aaagtttaat attttctgcc gagtctgttt
      481 ctacacatac attatcaaaa ttccatctga gtacaggatg attgttatgt ataatctttt
      541 ctttcagaac aagcgagtaa agttccttgg tcggaggtga catatcctta aagccttgtc
      601 cgaaaggtat catcgtcaat ccttcatcct gcaaattaag aattatctgc gttgcattat
      661 atctgtcata tgctatttca cgcacaacgt acttgcttgc aatatccttt atgtcatcct
      721 ctattcttcg gtagtcaact acattcccct ctgttgttcg tataaatccg tttgctttcc
      781 atacatcata cggaacatgg tcacgtcgta cacgatgatg aagattttct tccggtatcc
      841 aaaaatacgg aacaattata tatttttctg tatcgtttct cggagggaac accaaaacaa
      901 atgccgtaag gtcaagtgtt gttgaaaggt caagacctgc atagcattcc cttccgataa
      961 gtgtatcaag atttattact tcatcacacg catcccattt ctccatctgc atccatcgtg
     1021 ttgactgttt aacccactga ttaaggcgga gctgtctgaa cagattttcc tcagccggat
     1081 tttcctttgc cgacaaaaat gcggcacgga ctttttcaat gtcaactgta tgtccgagcg
     1141 agggatttgc tttatgccaa ttacgttcat ctgtccagtc atctgtatct tctattccgt
     1201 atataacagg ataaaatgtc ggatcgattt ttctgccttc caaaatgtca actgcctttt
     1261 ggtgctgttc aaaacatatg ctgtttcggt ctgttccggc agtagttata agaaagaaca
     1321 acggctgtgt tcgtgcatca cctgaacctt ttgtcattac atcaaacaat tcacggttcg
     1381 gctgtgaatg cagttcatca aatattacgc cgtgaacatt aagaccgtgt ttcgtgaaag
     1441 cctcgctcga cagtacttga taatatgaat tagtcggttt atacacaagt ctttttactg
     1501 acataacagg tttaattctc tttttcaaag caggacattg ttcgaccata tccaccgcca
     1561 catcaaacac aatacttgcc tgctgacggt cacttgcaca gccgtatact tctgcacccc
     1621 attcaccgtc accgcatgta agatacagtg ccactcctgc cgcaagttct gacttaccca
     1681 tcttcttcgg tatttcaaca tatgctgtgt tatattgtct gtatccgttc tcctttaccg
     1741 taccgaacac atcatttatt attttatcct gccacggcag caattcaaaa ggcacacccc
     1801 gccatttgcc ttttgtatgt ttaagtgcat ttataaatgt aactgcacgt tttgctttat
     1861 tttcgtcata cattactttt tctctccgcc caatgaaaga agctgttcca ttgcgtcatc
     1921 actgctgtca ttgcctctgt ctgttacaat tcttgacctt gacgcaggag taagaccgaa
     1981 ctgttcacag aacttgctca tctgtttcat atactgctga gcaatagata cctgcggcac
     2041 ttgctgccaa tatccgctcg gagttttgac aatagcaccg tgtcttgata taaattcttc
     2101 tgcttctttc caacgtgcat aagcttggca atatcccgca aaagccgcca tatcaacttc
     2161 tgttagaata ccgagctgtt ccatctgttt cgcaagacgt ctccattcct ttttcgcatc
     2221 atcttcaagc catttcggac aagacggtgc acgagccgca ggtttcggct catttgcgtt
     2281 taactgtctc ttgcctggat taccctcaag ctgttttact gccgttggtt tcggctttct
     2341 gcccctctgt gccaagcgta tcacctccat tcgtataaat ttcttgtcat taaaaatttt
     2401 catacaaaaa accgccattt aaggcggttt aaaaattgta ttttcttatt tttctgtaat
     2461 cataatctca tattcatagc tgtttagttc aaaaaggtca actactttcc ataaatcata
     2521 ttggtcagga atatctttaa ctgttccgca gaaataaacg tctgtatgtt tttcattaac
     2581 caattttatt gttgcgtttc ggttgataag gctgtaaagt ttctttaatt ttagcatttt
     2641 gttttcctcc gttttctcgg tctgtttatc tttttgttgt acacatatta ccgttatgtg
     2701 aggactttat caataccatt actatacaaa aagtacagcc tttatttgtg tatatactac
     2761 cctcgtattc ccttaaagtt atagtttccc ttgcgaattt ctgcaatgtc ggcgtcttgt
     2821 gactttttgt attccgaatc ggtacattct tttttcttgc agtccataca gatacatttg
     2881 ctattaaaca tactttg
//
LOCUS       DAWACS010000153         2875 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3646_length_2875_cov_2.842199, whole genome shotgun sequence.
ACCESSION   DAWACS010000153 DAWACS010000000
VERSION     DAWACS010000153.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3646_length_2875_cov_2.842199"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2145..2154
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          895 a          420 c          655 g          895 t
ORIGIN      
        1 taagacagat tattgaccta ttactaaagg atattcacgc aaaacttgcg gaaaaagacg
       61 caaaacttgt tgttaccgat gaagcgaaag agcttatctt ggaaaaaggt tacgataagc
      121 gatacggtgc aagaccgctt aagagagcaa tccaaaaact tttggaggat aaactttcaa
      181 tcttgtcgct tacaggtcag attacatcgg gctgtgtgat caccgccgac agaaaagaca
      241 acgaaatcac acttacggca gtttcaaact tgcttgaaaa ttagtatgat tttcctgtga
      301 tattttaaac ctaactgcaa ataatattta tgtgccgcag gggcggtatt aaatattatt
      361 ttatggaggt aaaatatcat ggcatgttgt aacggcgaaa agaacgaaag tattaaatgt
      421 actgtaacgc agtgtgcaaa tcactgtgat tcagcagatt attgttcact ttcaagtatt
      481 caagtgggta cgcacgaggc taatcctaca atggtagaat gtacagactg taactcattt
      541 gaattgaaga agtaataaaa aaatttcaga accctaaaag gaactgtaga aaaattatgt
      601 tttttctaca gtccctttgt ttatggagaa aattatggag aataaattta agtattcaaa
      661 cgacaataaa agataccata catggaacta tcatcttcag cagaaattcg gcggtaaggt
      721 gtttaaggtg gcgttaaacg gcggttttac gtgcccgaat atcgacggta aaaagggcgt
      781 cggagggtgt atatattgtt cgtcaagcgg cagcggtgat tttgcgggtg atccgtcaca
      841 taatattctt acgcagtttg aggaagtcaa ggagcgtatg cataaaaagt ggcacgaggc
      901 taagtatatt ccgtattttc aggcacatac aaatacatat gcacctgcaa gcgtgttgcg
      961 tgaaaggttt gagcctgtgc tgaaacagga taacgtggtt gggataagta ttgcgacgcg
     1021 tgcggattgc cttgaagatg atgtgcttga ttatttgtcg gaattgaacg aaagaacata
     1081 tcttgtgacc gaactcggct tgcagactgt ttttgacgaa accggtgaaa agattaatcg
     1141 ctgccataca tatgcggagt ttttggacgg ttataaaaaa ttgcaggata gaaatataaa
     1201 tgtgtgcgta catcttattg acggcttgcc cggtgaaacg acagatatga tgcttgaaag
     1261 tgcaaaggcg gtggcgaaac taaatccgca ttgcgtgaaa ttgcatttac tgcacgtttt
     1321 gaaaaatact cgccttgcag atatgtacgc aagaggtgag tttgaactta tgactcttga
     1381 agattacgtt gaaacgatag taaatcagct tgaagttttt tcggaagaaa cggtaataca
     1441 aagacttacc ggtgacggcg gtcgtgattc acttgtaggt ccgctgtgga gtttgaagaa
     1501 atttgtagtg cttaatgaaa ttgataaact tatggttaaa cgcgatacat atcaaggggt
     1561 taagtatggt gaatagtaaa aaagagaagt caatggcttc tctttttttg ataaaagttt
     1621 tattgttgta attctattct atcaagatac aatgcaggaa ctgtgatttt tccgcccata
     1681 gtcgattgat aagaataaag tcctaaagaa gttccgtaaa ccgttacata atcatcatca
     1741 agaattcttg atgttactat atttttcgga taagcaacat atataacgtt gtcataatct
     1801 ccgttcacag caattctgtg ttgagtttca gtatcacctt ctataacttg aatgacttta
     1861 ccgctatatg taaggctttt tcctttatat gggtcaggat tccttgcaag ttctttgtag
     1921 tcaacataac cataattttc tcttataaat tcctttgcaa cgggagcagg tgtcggtgtt
     1981 ggcagaggtt ccggagtggg agtagcaata ggtacgggtg ttgccgactg ttgttcggtg
     2041 acaggtgctg ttgtgacaac agttgaatct ttttgggttg gttcgtcaga tgaagaacat
     2101 tttgtaaata aaagaataat aatgataata gccgcaatta taatnnnnnn nnnnttataa
     2161 ttgtgataat gtatttttta tttggattat tattcccttg aggaacttgt tgtataggtg
     2221 atgtgtctgt tgtttgtgtg ttttgttgcg gtgtttgagt cgattgagta taggcggttt
     2281 ctgattgagt gttttttgtg tcggtatttg ttgtttgctc aactttctct aacggtgtat
     2341 tgcaagcagt gcagtaaaca atatcatcag taaattcttt tgagcattta ggacattttt
     2401 tcataagtgt tccctccata tatgtatatt ttgcacctat ataggtgcaa aattattata
     2461 acacaatgct acaatgaagt caatagtttt gtacttatat tagtgcattt tgacggggtg
     2521 aaatatggac gcattatatg agaaaaaaat aggaaataaa atccgaagta ttcgtgaaaa
     2581 aagaaagatt acacaagagg aattatctgc aaaactgcaa gttagagggt gtgacataac
     2641 acgtagtgca cttgcaaaaa tagaagttgg tcagcgtcat atatatcctg atgagattag
     2701 gcattttaaa gagatactaa atgttgaata tgatgattta tttaatgtgt gagataatta
     2761 ttaggataga ttaaaaagag aagtcaatgg cttctctttt tgtgtgagta ggggcgaaca
     2821 ttggtcgcct gtgtagcaag gtaatttgcg gtggcgataa aaaggggacg tcgag
//
LOCUS       DAWACS010000154         2872 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3650_length_2872_cov_2.609159, whole genome shotgun sequence.
ACCESSION   DAWACS010000154 DAWACS010000000
VERSION     DAWACS010000154.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3650_length_2872_cov_2.609159"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          690 a          691 c          520 g          971 t
ORIGIN      
        1 catagtattc tactccgtca gcaactacat ctgccggaat aaccgctgta tattcatcat
       61 tttcctttac ggtatccata gtcttccatt ctcttgccgt atatgcagca ttgtttttac
      121 tatatacgag ctttacggaa aacggatctg catcagaatt tacctttata tgaactttat
      181 ttgtaccatc ctgttgcaaa acagtttttg ttatttccgt ttttgacgga gctgttccct
      241 gccccttaag gtaataatcc ataaaggtat atactgagtc acttccgctt atactttcgt
      301 gactcgaatt ataactccat acgtgatttg cgcttgccgc attacccata tttgctgctt
      361 tattcaaggt agcttcaact gatttgggtc tgaagaaggt gtcctttgcc gcagcgtcca
      421 tatagtagtt tgccgttata ttttcaagat aagccgaagg atcaagatag ctgtaccatt
      481 cctcaatggc aaactcatct ttcggaaaat atccattgtc tgtccagaag ctctcgtact
      541 ttgtgccatc agatgtagga ccgtagaagc ccgaaccgta ctgcgaataa gctgccttta
      601 ccttgtcgcc gagaatacct gttacaaagg tcgttgagta tccgccccat gagcttccca
      661 taatgcccat tttgtctgtg tctgtctttg aatttgacgc aagataattg aacgcctgta
      721 ttacgcctac ttcagcgtca acaaggttag agttcttcgc tccgccgtcc ttttccgtaa
      781 tattaaagcg gtaattgtca tttcctatgt attgtccgtc cgagcgtgtt tcctcaacgc
      841 catttactgt gaccgttccc acacttcctg tcagcgcagg aatgtctatt gccattgtta
      901 cataaccctt tgccgcaaaa tcctttgcgc gttccttaag cgtgtcggct gtaccgccgc
      961 cgccatgcag aaataatata gcagggtatg ttccttctgc tgccggctca tacatatatg
     1021 cgaacatctt attttttata tttccgtatt cactcttttc ggttatatta tttatagtct
     1081 tatattcgcc gtcattaaga ccgctgttgc cgttatctct tgtgtagaac tgcgctgatg
     1141 attctataat atcagccgta actgcgtcac tcccaagcac tttctttgat gtatgcaata
     1201 ttgtttccgg ctctttaatc tgagacttca aaacaagctt tgtatctaca tacggcgcaa
     1261 atgggtctcc gaaatcatgc gcgccttcct gatggtctgc cgctaatgcc gtattaaaca
     1321 tacctaatgg catcagcatt gaggctgata acagccatga aataaatctt tttttgttca
     1381 ttgctttcat atcctttctt aatcctcata atttctgaat gccgatgccg gcaaatcatc
     1441 attgttaaac aacgttgcgt ctgtcggaag cttgtaataa ccgtaattaa catatttcgg
     1501 ttcacttata tcatcactcc atacaatgat actcttatta tcatccgcaa gctcatagcc
     1561 agataccgtc ttaaactctt tatcgtcacc cgcaagcgtc ataccgacaa gtgtgttgtc
     1621 tttaagctta agtccattca taacagaata gtcctcaaag gtgattttca gcgcttttgt
     1681 accgtcctct gttgtcacct gctctacggc cttgaatatc ggtcctgtat attcaagatt
     1741 ttcctcatta tatacaagct ttctcgccca caggctcaat cttctgccga cctcactctt
     1801 atttctcggg tgaatatcct ttatgttgtc agtattatct gtaataaccg ccatacctac
     1861 attttcaggt tttccgttag tggtattctt gcctatcata tagttaaact gcgcctcgcg
     1921 catagctgcg tattcgccgc ccggattatc aaatgcggca agctgaacat acataaacgg
     1981 aagactatcg tcattaaata ccgtgcggta atcacttacc attgattcca tagcctttgt
     2041 atatgccggc atagttatct tgctgctgtt ggcatcagct tcgccctgat accaaatcac
     2101 tccggcaagc tgaatacctt ttcctgtctc actgttttcc cagtttgtat atggatatat
     2161 cagtctttta tacagcgcag aattaccaaa ctgtgcgcca ttgccattat ccacctggtt
     2221 tacaaggaat gtttctatgc ttgcgccgcc gactgccgag gatattaagc caatcggaat
     2281 atggccgttg tcgccataca gatgctttcc gaaatacgaa gctgtcgccg agaatttaag
     2341 gtcctgtatg ctttgagtgt ttgactgttt ccagccgaca gtgtctgttt cccaataatt
     2401 tgataccgta ttgttttcat ttgatataat tcttatattt tcattatcaa gcaagccgct
     2461 gtcaacatca ttatggtagt cagtccaata cggatattca taaccgtatc tcatatttga
     2521 ttgtccggca cacaggtaaa catcaccaaa tataatattg ctgattgtag ttgtttttga
     2581 tgttgcatca cccgctgtag ctgtaagttc aagagtgtaa gttttgtcta cctgatacga
     2641 ttgttcaggt aatgttattg tccagtcacc tgctcttgtt gcggcagatg ttgcagtatt
     2701 gcttgctact tctgcgccac cttcatcttt aagtattgcc gttaccgttg ccgacttata
     2761 ggttttatcg tcattccgcg gcgtaccgtc aagtcgtccc cagatgttaa tttccttatc
     2821 acgctggata atcatattat ccgtgaacat tgacggcata tgtatttcaa ac
//
LOCUS       DAWACS010000155         2870 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3654_length_2870_cov_2.279574, whole genome shotgun sequence.
ACCESSION   DAWACS010000155 DAWACS010000000
VERSION     DAWACS010000155.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3654_length_2870_cov_2.279574"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2599..2608
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          930 a          526 c          704 g          700 t
ORIGIN      
        1 agaataacaa gcgagaatac tgataaatat tcgaattata agcgttggac aaattatgaa
       61 actgcggagg ttggcgttaa atataccgat aaatacggag aatggaagcg aacaactttc
      121 acctcacgcg aagataatgt aaccataaca aagattgaaa aatcctcggg aggacagctt
      181 ataaatgata ttatctctat tgataatatt tccgagatgt ataaagcaaa gaacggaaac
      241 agtagggttc ttgaccagag gtataaaaaa cttgtcggac aaaatgcgga ttatctcgcg
      301 cttgtttccc attatcccgt ctacacaggc agtccacttt atgacggcgg ttttggaaat
      361 gttataaaga ttatagcaga gggcgaaaat gctgaaaaag aagtaatttc tgataatgcg
      421 ggcgatagta tgctcgttga cggcaacaat tcgatacgtg tcaaaaatgc ggaggctctg
      481 tatctgatta ccgtgactga ccgtacattt gacctgacag accttgatga aacatcatca
      541 gcttctcagg tcatggagag atttaaggca atggaaagct atccgttcat tgataagatg
      601 gcagcgcaag caacagcagt tgccgagaaa tatacggctg gaggcaagtt tgattataat
      661 gcggctcttg ctccatccgc aaagaagcat agtgaagaat ttgaccgtct tacatttgac
      721 cttgacggcg acgaggaata tcaaacagct gacaataaca ctttgattga cttgcagcga
      781 ggaaccacgg acagaataaa ccatgagttt atggaacgcg catatatgca gtcgagatat
      841 gcacaggtat gctgcggcgg aacaacagct ccgcgtttgt acggtatgtg gacgggcgaa
      901 tggaatccgg gatggagagg aatttatact cttgatgcaa atgtaaattt acaggtaagt
      961 gcgatgaata ccggcaatct ccgagacttt cagttgggat atataacatt cttccttcgt
     1021 cattctccag attttatgta taatgcggaa atggcatacg gtatgcacga cgctatacag
     1081 ctttcagtaa atgcagatgc tgacagggct atgcatgttg agtatgacag ctactatccg
     1141 tttgagtatt ggaatgctgg cgcaagctgg tgtctgctgc cgatatatga gtattggcag
     1201 tgttttggaa acactcagat accgataaac gattatatga gaatagatga ccttcaaaag
     1261 gtacttggag ttaatgacgg cggtctcaca aacgaggagt ttgcggcttt aaaggcgaag
     1321 ggctatcttg accttgagaa ggatattttg cttccgctgc tcacaaagca ggctaatttc
     1381 tgggaacaga ttgtaacacc gcgctattac acagatgtaa acggtaaagc gcgccatgat
     1441 gagaataaaa agtcacttaa cgagggcgag aagtatatta taattcccgc atattcaccg
     1501 gagaacaacc cgataggcta cacaagcaca ttgacagcca atgcgacaat ggatatttct
     1561 gctgctcgtg acggacttga tatggtatgt gcattagaaa aggcagtagg cagagacggt
     1621 gcggacgaag caatcgtaaa atggcaaaac ctcaaaaaca aaatatccga ttataagacg
     1681 gatgatgacg gcgcgcttcg tgagtgggca atggaggaat acacggagaa taataatcac
     1741 cgtcatttga gccatttgta tgtggcatgg cccgcatatg acactcagaa agatccggaa
     1801 cttgcaaaag ctgcaaatat cgcacttgat aaccgtaatc gtttcaatac aggagatgcc
     1861 acagccggac atggttggat gcacaaggct ttggtagagt caagattaaa gcgcggtgac
     1921 ggaatggtag gttcactttt aaagatgatg accggtacag cgtattattc atcatttatg
     1981 acagatcatg ataccaaccg cagaaatgat acatattgta cggatactgc gttcggcagc
     2041 gtagctgcgg ttaatgaggc acttgcattc tcgaatacag gtgagattga gattattccg
     2101 gcacttccga cagattggaa cggcggaaca ataacaggac taatgactcg ttcgagagct
     2161 gaacttagta gtctttcgtg gaatattgaa gataagacgg ccgatgcaac gataacatct
     2221 aatatggatg aaaataaaat aaaggttcgc tgcggtgaat cgtggagcag cgctgctgtt
     2281 aacggaaaga aagccgaagt tctttcggac gaagtaggta aatatatact tgtaagcctt
     2341 gataacggag aatcggcaga tattaatttc tcgcttcttg aaagaaaaga aaatgtttac
     2401 gaaaaggtgg agatttcttc gtcaaacgct gctttggccc atgacggaaa cgcatcaact
     2461 gctgcatcgg ctacgagcgg tgaatttgta tttgacctcg gaagaacgat agatattaat
     2521 aaaatcgtaa taaaaaagca agcaaatgtc ggcggtacta cttataatta ttggggcgac
     2581 tgggtacttg ccgtaggcnn nnnnnnnntg tgagctgcag ggaagcacgg acggtaaaaa
     2641 ctggaagaaa ataatgacaa tgaatccggc gccggacaac aaaaatgacg aatcagaaga
     2701 agtatatata ccttatacac ctgaagtcta cagatatata cgttatgtga gaactgagga
     2761 gaagaactca aaatcatacg gagcatgggt gttcccggat gataacggca acagattgaa
     2821 tcttgccgaa atagaggtat acactgacag tctttataaa aatacggcac
//
LOCUS       DAWACS010000156         2864 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3665_length_2864_cov_2.263795, whole genome shotgun sequence.
ACCESSION   DAWACS010000156 DAWACS010000000
VERSION     DAWACS010000156.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3665_length_2864_cov_2.263795"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          824 a          550 c          576 g          914 t
ORIGIN      
        1 attttgagtg gttgcgttta aatcgttgct ttcaagaaat ttcattgttt tatctctgca
       61 ctttgcggcg gctgttgcaa cgagccagcc ttgcatcatt tgaacatagt agaaatctga
      121 atttaccgag ttgacgtatg agagcacttt gtcgatatat tcatcggtca aataaaattc
      181 cataaggcat ttaaaaccaa aacgtacaat ccatggattt tcgttgtaga taaaatattc
      241 aacgtcattt aaaaattcat ctaaatgttt cttcaatcct ttaaatgaaa caatgtcgca
      301 agtttcccaa cttgtgattt gaggaacgta ttgcttgata tagccgagca tttcgtgata
      361 atcgcactta ataagcgaca taaccaagcc tttaatcata atttcttcac ggtatgcgtt
      421 gtttttgcaa ttaaggaatg acgcgtagtc accttttgaa atagctttag ccgtttgacg
      481 caagatgggt acacgaatac cgatagattg cgttccgccg ggaacaagcg agtcactgaa
      541 ttttttgtat ttttcgtcag ccaatgtttt taagtattcc gcaaattcgt cataatctga
      601 ttgcgtccat atttcttttg taagctccat tatttgtcct ttctttgtct gaaaataaaa
      661 gagttgtttt ggaataaatc taaaacaact cttaaaatca attattcacc gaaaccgtca
      721 gggtgagttg actgccatcg ccagctgtca gcacacattt cgtcaatacc tctcttagcc
      781 tcccagccaa gttcagcctt tgcctttgca ggatctgcat aacattcggc aatatcgccc
      841 ggtcttcttg ggtcgatttg ataaggaatt tccttaccgc aagcctttga gaatgcctta
      901 acaatgtcaa gaacgctgta accgttacct gtaccaaggt tgtagatgtg tacgccttcc
      961 ttgtctgaac aatgttcgat agccttaacg tgaccgattg caaggtcaac aacgtggata
     1021 tagtcacgaa cgcctgtacc gtcaggagta gggtaatcgt taccgaagac atgaactttt
     1081 tcaagcttac ctactgcaac ttgtgcaatg tacggaagta agttgttcgg aataccctta
     1141 gggtcttcac ccataatacc gctttcgtga gcaccgattg ggttgaagta acgaagaagt
     1201 gttacagata gctttggatt agctttttga gcgtctgtca taattctttc gataaatagt
     1261 tttgttgttc cgtaaggatt tgttgtaaga attttgtcgc ccttgttgtc agtttcaaca
     1321 ataggaactc tttcaggatc gccgtaaaca gtagctgatg atgagaatac gatgttgttg
     1381 cagtcgtatt tttccatagc ctgcataagg tgaagtgtac ctgtgatgtt gttatcataa
     1441 tatgtaagcg gtattcttgt agattcgccg actgccttta gaccggcaaa gtgaataact
     1501 gcgtcgattt tattttctgc aaataccttt tctgttgcct ctttgtcaag caagtctact
     1561 ttgtagaacg gaaaatcctt tcctgtaagt ttttttactg cgtcaaggca tttttcactt
     1621 gcgttgtaaa gattgtcaag tacgacaatt tcataacctg catttaaaag ttcgacacaa
     1681 gtgtggctgc ctatgtatcc tgcaccgcct gttaccaata ttttcattag cgattcctcc
     1741 ctatatttat ttaattttat tatataccaa tataaagcat ttttcaatac tgaaaaatat
     1801 ttttgtgggg attttataaa aatatgtggg acgtcggggc accgtccttt acgaacgaaa
     1861 ttttttttat tctggagacc aatggtcgcc tcgacgtaat attatcgttt tggcacccct
     1921 cagtcaactg cgttgacagc tcccctcaaa gggagcctaa aaagcctctc cttgaggaga
     1981 ggtggcacga agtgacggag tggtggtatt tatcacccat taacaaaacc gcctatattc
     2041 catattatat taatataaaa aagaaaggaa tgatatttat ggaatataac agagagaatg
     2101 cggttgcata tgcgaaaaag tgggcatacg gaagaaatcc gaaatactat gatttttctg
     2161 atttgggcgg tgattgtaca aactttgcgt cgcagtgcat atatgccggc agcggtgtta
     2221 tgaactacac gccgacatac ggttggtact acatttctgt aaacaacaga gcgccggcat
     2281 ggacaggagt tgacgaatta tacagatttt taaccaccaa ccgcggtgca ggaccgagag
     2341 caatattaac cgatttatca caaattcaaa acggcgacat tattcagctg caatttaccg
     2401 acaaagaaag atttgaccat tctccggttg tggttgacgc aggaaacaga acaccgcagt
     2461 caattcttgt tgcggcacat tcgtatgatg cggattgcag accgctgtcg tcataccgat
     2521 atataaagat acgtccgtta catattacga atgtaggcga gtgaaaatat taatattaat
     2581 acacattttt cattgaatat ttttagaaaa agtgataaaa taaaaaggat attgattttt
     2641 cacaaaagag gtgttttata ttgaaaaaac tatttccgtt tctgaaaccg tacaggcttc
     2701 agcttacagt cggaccgttt tttaaattgt ccgaggcggt gttggagttg ctaattccga
     2761 ctcttatggc tttgctgatt gataacggag tcaacatgga taacagaagc tatattatta
     2821 aaatgggtat attaatgctt gtcattgcaa catttggcgt aata
//
LOCUS       DAWACS010000157         2861 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3674_length_2861_cov_1.919102, whole genome shotgun sequence.
ACCESSION   DAWACS010000157 DAWACS010000000
VERSION     DAWACS010000157.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3674_length_2861_cov_1.919102"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1967..1976
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1027 a          366 c          637 g          821 t
ORIGIN      
        1 attgatgatt tgccgccgtt tgaggatgat tcaatggaaa acagaacata taagttcttt
       61 aagggaaaac ctgtttatga atttggttat ggacttacat actcaaatat aactgaaaat
      121 tggattgatg ataaaactgt tgaaattgaa aataaaggat atattgatac cgcatacagc
      181 gtgctaagat atgaatacat tcctcataaa aatctctgcg gatttaaaaa gatttttctt
      241 aaagcgggag aaaaagaaaa atatatcttt ttataaatga tatatttcgt acaatagcta
      301 tttgacaaat tgtctcaaaa gtgatatatt aaaataagaa agaacacagt ttaagatata
      361 ttattttgga ggaaaataca aatgtcagat aagaagccgc tatatatgca ggttatagat
      421 aaattaaggg aacggattaa gtctggagat tttgaatatg atgttccgtt tgtgactgag
      481 gacaggataa caaaggaatt cggtgtaagc cgtattactg ctatacgcgc attggaagag
      541 cttgaaaagg caggtataat taacagaaaa agaggcagcg gaagctttgt ttcgcgtgaa
      601 gcggttgata ttatagaagg cagaagtgtg tctgatatca ggacaaattc ggaaaataaa
      661 gacagagaag tattacttgt agcattagtg ctgccatttg acataaagct gggaaatatg
      721 tttaaatgtt ttgatggtat taacagtgtg ttaaataaag agaattgttt tgccagtata
      781 tataatgcaa accgggatgt tgaaactgag gcgagtattt tgcgctctct tttaaatcaa
      841 ggtatagacg gagttatatg ttatcctgta aacggcacaa agaactttga ggtatataac
      901 cagtttcttg caaaaaaaat tccgcttgtt attattgata attatattga aaatatgcct
      961 gtgagttatg ttgcatcgga taacttcggg ggaacaaaga tgctgtgtga gtacgctata
     1021 agtaaagggc ataaaaaaat aggctttttc agcaaacgaa gaataaatga gtcgttgtcg
     1081 atacgcgaca gatatatggg ctatacgtct gctcttgctg agaataatat tgaggtaaat
     1141 cttgattatg tatgtgttga tcttgacgat aagtataata tgcttaatga aacagaggcg
     1201 gctcggtatg acagtctgag agcgtatatc gctgagatta taggacaaat gcgcgcagaa
     1261 ggagttacct gtattttatg ccagaatgac tgggttgccg ttgagctgta ttctgtttgc
     1321 gaagaacttg gaatatcagt tcctgatgat atgtgtatta tgggatttga caatatggac
     1381 gaactcaata atatgcatgg cggagataaa ataataaccg ttgaacagga cttttatgag
     1441 ataggtgtga gggcaggaga aacaatactt aaagaaatcc gtggggaaga aaagggtata
     1501 agagatgttg taccggtaaa attgattaca cgggattatt aacagaataa atattggata
     1561 attcaaggat atgtgagatt gaacttttca catatctttt ttgttaagaa aatataataa
     1621 atacaacaca aatacaagaa aaaaacgaaa aaacacttga ctttgatata ttatattgat
     1681 ataatttata tatcaaatgt tgaaaatctg caaaaaccgt agaaaatttt attacagtgt
     1741 gaggtgggac caatatatga aaaaaatacc tttatattca aaaattatag acgagttaaa
     1801 ggaacgaata agaacaggtg attttgaata tgatgttccc tttgtgacag aagaaaaaat
     1861 aacaaaggaa tacaatgtca gccgtattac cgcaatcaga gcacttgatg agcttgagaa
     1921 gataggagtt atatacagaa aacgcggcag cggaagtttt gttacgnnnn nnnnnngcat
     1981 agaaatactt gacggtgtgc agaatgatac taacgagaaa gaaactgtgt ttattaaacg
     2041 aaataaagaa ctgttactta tcgcgcttgt gcttccgttt gatgtaaaaa gaggcggaat
     2101 gatggagtgt tttaacggaa taaatgattt gctcaacagt gaaaactgtt ttgtacgtgt
     2161 ttataataca aacgggaaaa acgagactga ggcaaccgta cttagggaac ttttggacaa
     2221 tgacatagat ggggttatct gttatcctaa aaatgataat aaaaatctcg aaatatttaa
     2281 tcagtttctg acgaaaaaca taccacttgt acttattgat aaatatatag aaaatatgcc
     2341 cataagctat gttgtgcctg acaattataa cggcgcacgg gaactatgca attatgccat
     2401 agaagctggg catgacagaa taggattttt cagcgtgact gatttttcag gagtttgctc
     2461 gctgcgtgaa cgatatatgg gatatgctat gtccatgaat gaccacggca aagaggttaa
     2521 ccttgacgat gtgctgatag gagaccgaag aaaagatttt tactgcgaag gtatgaaaaa
     2581 cgacggcgaa tatttaaggc atgcggtaaa aaagctttat aagtccggaa caaccgcgat
     2641 tatatgccag aatgactggg ttgcgagaga tattattcat tgctgtgatg aacttggaat
     2701 atcagtccca gacgatatgc taattatggg gtttgatcat gtaaacgctt ttactcctat
     2761 tgatgttggc gaaaggataa ctacggtaga acagaacttc tatgaaatag gtaaaaaagc
     2821 gggagaagtg attcttaaag agttgcagga aagaaaagga c
//
LOCUS       DAWACS010000158         2837 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3714_length_2837_cov_2.620776, whole genome shotgun sequence.
ACCESSION   DAWACS010000158 DAWACS010000000
VERSION     DAWACS010000158.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3714_length_2837_cov_2.620776"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          835 a          539 c          559 g          904 t
ORIGIN      
        1 agcttgtagc ctgtaactac accatggacg gaattaaaga acttatcggt gcggacagta
       61 tcggattttt aagcgttaat tctttgaaaa agattattcc taacgcaaaa tgtcaattct
      121 gtgacggatg tttctcgggt aaatatcctg ttgatgttga ctgataaaat ttaataagaa
      181 agaatgaggt aaatgattac tgacttgaca gagggcaaac cctcaaaaat attatggagc
      241 ttttcgatac ctatgctttt gagcgtggta tttcagcaac tctataatat agtagacagt
      301 atcgtagccg gaaaatttgt aggcgcccag gcattagctg ctgtgggcgc ctcttatccg
      361 attacgatga tttttatggc aatagccaca ggtgcaaacg tcggtgcggc tgttattatt
      421 tcgcagttgt tcggtgcgaa aaattttgaa aagctcaaaa cttctgtttt cacttcaata
      481 atttcaatga ctgtacttgc cgcaattctt acaatcttcg gaattgtttt ctgttcggga
      541 atgttaaaaa tgttatctac tcccgaaaat attttttccg actcaatgac atatcttaat
      601 atttacatag gcggattgat ttttctgttt ctgtacaaca tatgcacagg tatttttacc
      661 gctatggggg acagtaaaac tccgttgtat tttttgctta tgtcgtcagt cggaaatata
      721 attcttgacc ttgtttttgt aattgtattt aatatgggcg tggctggagt cggctgggca
      781 acatttattg cacagggtat atcttccctg ctcgcattcg ccgtactttt aaaacgcgtt
      841 cacggtataa aatcggggac atataaaaaa ttttcaatcc gtatgctcgg acatatttca
      901 agaattgcaa taccgagtat cttgcagcaa agttttgtat cggtcggaaa tttatttata
      961 caaagtcgtg taaacagttt cggctctgac gttatcgccg gttacagtgc ggctataaag
     1021 ctgaatacat tcgctataac aagtttttca acgctcggaa atgcaatgtc aagctatacg
     1081 gcacaaaaca caggtgcgcg aaagcttctc cgcatacctg aaggacttaa agcaggtatt
     1141 gttatgctta tcgttgtttc tctgccgttt ttcattgcgt atttcgtatt cccgaatact
     1201 atgatgaata tattcgtaaa aagcagtgag acaggtatta tagacgtagg aagaatattt
     1261 ttaaaaatcg tcacaccgtt ctattttgta atttccgcaa agcttacatt tgacggtatt
     1321 cttcgcggtg cgggacgtat gagggcgttt atggcaagca cgtttaccga cttgctttta
     1381 agagttattc ttgcgtatgt gttctcatac gcacttaatt cggaagtcgg aatatggctt
     1441 tcttggcctg tcggctggac tatcgccgca gttatttcgg cagtattcta ttttatttat
     1501 tataaaacaa caatcaaaaa cggtgcaaat taattgcacc gttttttagt tttcctcaat
     1561 atgaataagc atttgttcta taatcttttt aacgtggtcg tcatcaatcg aataaaaggc
     1621 atttttaccg tcacggcgtg actttataag ccttgcctgt tttaaagttt taagctgatg
     1681 actgatagcc gactggctca taccgagcgc gttcgctata tcatacacac acaattcctt
     1741 atcaaacaac gtccataaaa ttcttattct tgtactgtcg ccgaatactt taaacatttc
     1801 cgacaattta tccgttacat cttcatcagg aagttgattt ttcaatctat ctaaaatctg
     1861 cggattatat tgacaggtac tgcatttatc catttatctt ctcctctttt cggtcatatg
     1921 ttcatatatt catattatac ccttttaatc tttatttgtc aaatcgcttg aaaaatcaga
     1981 tattaattta taaaaatcag aattcattat taaattttcc caaattcttt ttataatatg
     2041 ataaaatata gtttagatac tttgaaaagg aggattttaa tacactatga ataaaaagaa
     2101 aatcatttcg gctatgctta cagttgcaat gcttgcaagt tcggctgttt ccgtgcttgc
     2161 cacagaggta ggcgaaagcc tgaaagtcgg cgattacaca gaatccgccg ccgaaacaca
     2221 aaaattaatc gactcacgac ctgatattca gcgtcctatg gaaaatctaa acagaggtgc
     2281 tgttgccgta agaatggacg gctatacata tatttcgtgg cgttggctcg gtactgaaag
     2341 tgcggatacg agatataaca tttatcgcgg ctcaaaagag atgtcctctt acggacaaaa
     2401 attaaataca gagccattaa atgtaacaaa ctttaccgat gtgtttactg ccgattctaa
     2461 cacacagtat tgcattatac ccgttgtaaa cggtgaagaa caggcagaca aggctgaatt
     2521 ttctcaaact tgggataaca attatatgga tattccaatc caaaagcccg aaaacaacaa
     2581 agtaaacggc gaagaatatt catatacacc gggcgacgcg tctgtcggtg accttgacgg
     2641 tgacggtgag tatgaaatcg tactaaaatg ggatccgtca aacgcaaaag atgccgcaca
     2701 ggcaggtttt acaggcgaat gtattcttga cgcatacaag cttgacggca cacgtctatg
     2761 gcgtataaat atggggccta acattcgtgc aggcgcacac gatacgcagt ttatggtata
     2821 cgactatgac tgtgacg
//
LOCUS       DAWACS010000159         2819 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3741_length_2819_cov_2.682706, whole genome shotgun sequence.
ACCESSION   DAWACS010000159 DAWACS010000000
VERSION     DAWACS010000159.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3741_length_2819_cov_2.682706"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    760..769
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          833 a          644 c          488 g          844 t
ORIGIN      
        1 ctacaagacc ttgtgatgtt gtgtattcaa acggtgggaa tgctgcgttt gtacccataa
       61 tcaacttatc actgcttccg caacctgcaa gtgctgccac tgccataact gatgcagctg
      121 ccaaagctac tactttcatt aaaccctttt tcattttttg tatcctccta aaaaatcact
      181 tatttttata tttaacacat tctgtgttag ataactactt taaaactttg ctcaaaaatt
      241 cttttgttct ttcgtttttc ggattgctga acacttcgtc aggagtgcct tcttcaagca
      301 catatccgcc gtccataaac aaaactcttg atgcaacttc gcgtgcaaat cccatttcgt
      361 gagttacaac aaccattgtc ataccaccgt ccgcaaggtc tttcataaca gaaagaactt
      421 caccgaccat ttcagggtca agtgccgatg taggttcatc gaacaacatt atatccggct
      481 gcattgcaag tgcacgtgct attgcaattc tctgctgttg accgcccgaa agctgtgcgg
      541 gatatgagtc ctctttgtcc gcaagaccta ctttattcaa aagctcacgt gcaagctttt
      601 cagcctctgc tttcggcatt ttcttaacct taattggtgc aagagttata ttgtcaagca
      661 ctgtaaggtg cgggaacaga ttaaactgct gaaacaccat acccattttt tcacgaattt
      721 tatttacatt tgtttttgga gcggtaatgc tttcgccctn nnnnnnnnnt caccttcctt
      781 tggctgcagt ccttcgccga tagcggtaat gctttcgccc tcaatgagaa tttcaccctc
      841 tgtcggtact tcaagcaaat taagacatct caaaaatgtt gatttacccg aacccgaagg
      901 acctataaca acaacctttt cgccttgttt tatgtgctgg tcaattccct ttaatacttc
      961 taaatctcca aaatgctttt ttaaaccggt aacctttatc atctcttatc cgcctttctc
     1021 ataccttttt caactttaga aagcaacgta cttataatct ttataacaac ataatatata
     1081 actgctattg acaaaagcgg tataaggtaa tcatatgtaa ggctctgtac ctttcttgcc
     1141 gctccgacaa ggtcgatatt cgcaatcata ccgattacgg ctgtttcttt tatcaataca
     1201 acaaattcat taccaagcgc cggcagaata tttttcatag cctgcggcat aacaatgctt
     1261 gccatagtct gtttatgcgt cataccaagc gaacgtcccg cctcagtctg accgatattt
     1321 acggacaata taccgcctct gacgatttcc gcaatatacg caccgctgtt tataccgaat
     1381 gcaataaatc cgactaccca cttatcaata tgcacatttg caaaaataac aaaataaatt
     1441 ataagcagct gcaccattgt cggcgtaccg cggataatat caatataaat tcccgaaacg
     1501 gctcttaaaa tcttactttt tgacagacgc ataagtgcca tcagaatacc aattaccaaa
     1561 cccaacaata ccgagaaaag agatattaaa agcgtatatc ccaatccact aacaaaccac
     1621 tgccaacgtc cgtctaccac aaatgtagag ttcaagcggt ttaacaaatc aacaacccaa
     1681 gcaggcatat cttcaactcc taatgtattt ttatttcaat actctatata atatcaaaaa
     1741 ttgccctaaa tatcaatact tatttgaaat tttctacatt ttgatgtata acaattcatt
     1801 ttaggattgc aaaaaggtta aaataatgat ataatatata taagactggt ttagggaggg
     1861 atattatgaa taacgatatg ttcggaaaat gcggtatgac acagtttgtc ggtgtcggcg
     1921 tttgcgatac aatcggtctt tatatcgcca atattgatga cgatttaaga acgcaaatga
     1981 atatcccgaa aaagttccac gcggtcggtc ttatcggttc ccgtacaggt gcgggagcac
     2041 aaattcaagc cgttgatgac gcggttaaag ctacttcgac agaggttatc tctatcgaaa
     2101 ttccgcgtga tacaaaaggt tggggcggtc acggaaactt tatcataatc ggcggtgaaa
     2161 cacctgccga cgttaagaga gccgttgaat tagcgcttga atacatagac ataaatgccg
     2221 gcgaaatata cataagtgaa gcaggtcaca tggaattcca gtacagtgca aggtgcggtg
     2281 aggctatcaa caaggcattt gatgtgccga taggcagacc gttcggcttt gtatgtgcgt
     2341 cacctgctcc gattggtatg gtaatcgcag atattgcgtt aaaatctgcc ggtgtcgaat
     2401 tgataaaaac acttcgtccg gacagcggta caagccattc aaatgaagtg attataattt
     2461 tcacaggtga agcgtcagcg gttaaaaccg ccgtcacaga cgcacgttcc gcaggaatgc
     2521 aacttttgcg ttcactcggc tctgaacctt tgcctgtaac aaaaccatat atttaagcta
     2581 acaaaaaaaa tcacatcata tgatgtgatt ttttgaatat tatgtttttt tattttgaaa
     2641 ccagcacaag ctcatcattt ttttctatta tcgtacttcc gacaggaata acgacatcat
     2701 caccgcgttt tatcataatc acaagatagc cgtcgggaaa ttcaatttca gccatacttt
     2761 ttccacgcca attttttctg tcattcgctg aaatctccct aaaacgaaca tcaccgttt
//
LOCUS       DAWACS010000160         2818 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3744_length_2818_cov_2.924720, whole genome shotgun sequence.
ACCESSION   DAWACS010000160 DAWACS010000000
VERSION     DAWACS010000160.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3744_length_2818_cov_2.924720"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          857 a          503 c          645 g          813 t
ORIGIN      
        1 tcggaataca tccgcacgag gtttcagaac ttaccgaggc ggatatggat aagctgaagg
       61 aatatgcaaa aaatccgaaa gttaaagcga taggtgaaat aggacttgac tatttttatg
      121 atttttcacc gcgtgatatt cagcaaaagt ggtttgcaag acaagtggac gttgcacgag
      181 agcttaaaat gccggttgtt atacacgaca gagaagcgca taaggattgt atggatattt
      241 tgcgtgaaca taaggtgagc gaagtcggag gagtattcca ttgctatgcg ggaagtgttg
      301 aaatggcaaa ggaaatactt gattggggaa tgtatatcgc gttcggcggt tcacttacgt
      361 ttaaaaagtc ggtaagacct gttgaagtgg cgaagtatgt accgcttgac agaattgtta
      421 ttgaaaccga ttcgccgtac cttacaccgg aaccgcacag aggaaagcga aacagttcgc
      481 tttatatcca ctatgttgcc gaaaaattac aaaaagtaat ctgtctttta tattattcag
      541 ctgtttccgg agcaggctcg tcttcaatcg catcatatgc ggcaagaacc ttgctttcga
      601 gcatatttct catatcactg tttatcggat gagcaatatc tctgtactca ccgtcaggag
      661 ttcttctgct tggcatagca acaaacagct tttccggacc ttcaataacc tttacgtcat
      721 ggacagcaaa cgcattgtca aatgtaacag atgcgacagc tttcattttt ccttcgctgg
      781 gtactttttt aattctgatg tctgtaattt ccatgttttt catcaccttc cgacaaattt
      841 tactaattta ataatatagc aattcgctca atttgtcaag tgcttttcac agattttatg
      901 gtgaaaatgg cgaaaaaatt acaagatacg atttttaatt gggcaaattt ataaaaaagg
      961 gttttcaatt tgttgataaa ggtatataat taatatatta atatgagata cgaggggata
     1021 tatgatgaat agatttcaaa tgtctgatat agataagacg gcaaatatac attttatcgg
     1081 tataggcgga ataagtatga gcggtttggc acaaattgtg cttaaagacg gctacggcgt
     1141 ttccggctcg gactggaata agagtgctat aaccgaaaag cttgaaaata tgggtgcgga
     1201 tattgtgtac ggtcacggtg ccgttaacga ggacggcata aataaggcat cgcttgttgt
     1261 atatacggcg gcggcaaaag ccgataatcc cgaaattgtg cttgcaaagg aaaagggcat
     1321 tcgccttata gacagagccg agtttttggg tgcgataatg aaaaattaca agcacgctgt
     1381 cggcgtaagc ggaacgcacg gtaaaaccac gacaacatca atgcttgcac acgcgcttat
     1441 cggtgcaaat cttgacccta caatcagcgt aggcggtgag cttgatttaa tcggcggaaa
     1501 tatcagaacg ggtaaatcgg attattttgt tacagaggct tgtgaataca caaacagctt
     1561 tttgaaattt tatcctacaa tcgcccttat aacaaatgtt gaggaagatc atcttgactt
     1621 cttttcggga attgacgaga taatcgcaag tttcagacag tttgcgtact tgacaaagga
     1681 tataggttat gtcgttgcaa tgggcggaga taagaatgtt caaaaagtgc ttgaaaacgc
     1741 agacgacctt aacataatca cttacggaat ggaaagcaag tttgattatt accctgaaaa
     1801 cattgtgtat cacgcaggtt ttccgagctt tgacgttatg aaaaacggtg aaaaggtttg
     1861 tcatataaaa cttaatgtcc cgggcgaaca caatatttta aattcgcttg ccacggttgc
     1921 ggtatgtaat cttatgggcg ttgacgcgga aactgccgca aaggggattg aaacatttaa
     1981 aggtacgcac agacgttttg agaaaaaagg atttttaaac ggtgcggttg tgattgacga
     2041 ctatgcacac cacccgacag aaataaaagc gacacttcac gcggcacaaa aattcccgca
     2101 caataaagtt tggtgcgtgt tccagccgca cactttctca cgaacaagaa cattgtggga
     2161 cgaatttgtc ggtgcatttg atgatgcgga cgaattgatt ttgacacata tatatgctgc
     2221 acgtgaaaag tttgacggtg ttacaaaacc tgaaaatctt gcggaagata ttaagaaacg
     2281 cggagtaaat gcacagtata tagataaatt cgaggatatt gccgagtttt tgaaaaagaa
     2341 cgtcaaagag ggcgatattg tatttacaat gggtgcaggt gacgttgtta atataaataa
     2401 gttaatcgtt gagtaacaaa aaaagacgac ttctcagtcg tcttttttgt tgcctcccct
     2461 tgaggggaag tgtcacgagg tacgagtgac ggaggggact ttacttcgcc acaggtattt
     2521 taacggtaaa ttccgtatat tcattctttt ttgagtaaac cttaatactg cctttgcaag
     2581 cggttgtgat agcttttgct attgcaagac caagcccgta accgccgctt tctcttgctc
     2641 ttgacttgtc ctcacgatag aaacgttcaa atatttgttt ttgtgcgtct tcagaaattc
     2701 cgacacctgt atttctgact gtcaataccg catcagatga ttcgtgagta agagttatat
     2761 taatgtcgtc ggatttcggt gtgtatttga ctgcgttgtc aagaagtatc ataacaag
//
LOCUS       DAWACS010000161         2806 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3765_length_2806_cov_2.885496, whole genome shotgun sequence.
ACCESSION   DAWACS010000161 DAWACS010000000
VERSION     DAWACS010000161.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3765_length_2806_cov_2.885496"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          961 a          458 c          631 g          756 t
ORIGIN      
        1 caactaatta taaagacaag catcgtgacc ttaatactgc atttgaggca gtcggtgcat
       61 ttaaagcggg taatatcgat gaggacgaac ttctaagact tgaaaatgcg gcttgtccgt
      121 catgcggttc atgttcgggt atgtttactg caaactcaat gaactgtctg tgtgaagtcc
      181 tcggtatggc tttaccgggt aacggcacaa ttcctgctgt ttattctgaa agaatacgtc
      241 ttgcaaagca tgcaggtatg aagattatgg aacttcttga aaagaacatt aagccgagag
      301 atattatcac tccggattca atttataatg cacttcgtgt tgatatggca cttggctgca
      361 gtacaaactc aatgctgcac cttcctgcaa tagcacatga ggcaaaagca cctataacac
      421 ttgattacgc aaatgaaatc agtaaagaaa caccgaacct atgtcacctt gctcctgcag
      481 gtgaacatca cgttcaggat ttgtatgcgg caggcggtgt tcaggcactt atgaacgagc
      541 tttcaaagaa gaacttgtta aagcttgata caattaccgc aacaggtaag actttgggcg
      601 aaaatattaa ggactgcact gtacttgatt ataacgtaat ccgtccgata gataatccgt
      661 acagccaaac aggcggtatt gccgtattaa agggtaatct tgctcctgac ggctctgttg
      721 ttaagagaag cgccgttgca aaagaaatgt tgcagcacga aggccctgca aaggtttata
      781 acagtgagga agaggctatt gaggctattt atacaggtaa gattgtaaag ggtgacgttg
      841 ttgttatccg ttacgaaggt ccaaagggcg gaccgggtat gagagaaatg ctttctccta
      901 catctgcaat ctgcggtatg ggacttgata aggacgttgc tcttattaca gacggacgtt
      961 tctcaggcgc tacaagaggt gcggctatcg gtcacgtttc accggaggct gttgaaggcg
     1021 gtcctatcgc atttgtacaa gacggcgata ttatttctat tgatatagat aaatgccaga
     1081 ttaactttaa ggtcagcgaa gaagaattgg ctaagcgtaa agaaaattgg actccgcttg
     1141 aaccaaaggt taagacaggt tatctaagaa gatactcaaa gatggtttct tctgcaattc
     1201 aaggtgcagt tatgttagac gactaaagaa caaatgatta tacaaaaaac gcatttctta
     1261 tatggaatgc gttttttgta aggttgacaa atcaaatcat ttaaaatata atgtaggtaa
     1321 tatataatag aaagagagca agtatatgca agacggagtt aaacttgaac gtaagactaa
     1381 agatgaactg aaacaaatag cgcaaggtct tggaatagaa aaaatttcag ctttaaaaaa
     1441 agatgaaata attgtattga ttcgcgaaaa acgtgaacag attgcaaatg aggaaaaaca
     1501 gaaagtgcaa ccgaaaaaac gtgaacgtcc ggcagacgca gttgaagtaa agggtgtgct
     1561 tgacgtaatg acagacggtt ttggttttct tcgtatggag aactatatgt cgggcaaaaa
     1621 cgatatatat gtgtcaccta cacaaataag aagatttaat ttaaagaccg gtgacgaaat
     1681 tgtcggtgat tgccgacctt cacaagatga agataagttt tctccgttac tgtttgtaaa
     1741 gactataaac ggcgacccgc ttgaaattgc tttaaaacgc agaccgtttg aacgtcttac
     1801 accgatatat ccgaaagaaa aaataagcct tgatacaggt gacagaacaa gaaccgcatc
     1861 aagacttata gatttaatcg caccaatcgg aaaaggtcag agaggtatga tagttgcacc
     1921 gcccaaggca ggtaaaacta ctatattaaa acaaattgct caatctatat ccgaaaatta
     1981 tcccgatatt aaattgatag tacttttaat agatgaaaga ccggaagaag ttactgatat
     2041 aaaacgttca atagacggtg aagttatata ttctacattt gaccaaatgc ccgaaaatca
     2101 ttgtaaggtt gcggaaatgg tgcttgaaag agcgtcaaga cttgtggagc agaaaaaaga
     2161 tgtagtaatt ttgcttgatt caatcacaag acttgcaaga gcatataact tgacagttca
     2221 gccgacagga agaactttgt ccggcggtct tgatccggac gcattgtata agccaaaaaa
     2281 gtttttcggt gcggctcgta atatagaaga gggcggaagt cttacaattt tggcaacggc
     2341 acttgttgaa acgggcagca gaatggatga tgttattttt gaagaattta agggtacggg
     2401 taatatggaa ttgaaactcg acagaaatct tcaggaaaaa cgaattttcc ctgctattga
     2461 tattttgaaa tcaggtacaa gacgtgaaga agatttgctt aatgaccgtg aaaaagaggc
     2521 ttgctggatt ataagacgtg agcttggcag aaattcaaat accgaaacta ttgtcagctt
     2581 tttgcagtat atgagaagta caagcagcaa taaagatttt gttgagttga ttattaataa
     2641 ttacggaaga aaataatttt ttgagaactg taacgatttg ttacagttct ttttttgttg
     2701 tgatcgacgc aaatagtgta tgtaataaga aataatccac aatatatagt ataatgaaaa
     2761 aaagttcaaa aaaaatgaaa aaaagtgttg acaaaagggg taatag
//
LOCUS       DAWACS010000162         2777 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3809_length_2777_cov_1.965834, whole genome shotgun sequence.
ACCESSION   DAWACS010000162 DAWACS010000000
VERSION     DAWACS010000162.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3809_length_2777_cov_1.965834"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    571..580
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2012..2021
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          848 a          567 c          498 g          844 t
ORIGIN      
        1 atattcaggt tgttatctgt tttctgttcg attgtaatgt taatgtcctg tcttacgttc
       61 agcactgctg ttgctgatga gcctgtcggc aagatggcgg cggtatatgc ttatgtactt
      121 aataattatt tgtacactta cagttcaatg cacaccgaaa acgcaggtga ttctctttca
      181 atttcggagg acaatatacc taacggcgtt gtgtacagtg atatagtaaa ttttgacgca
      241 aatgaaaatc cgtatcttgt tattttcctt gcggacagcg gatacactac cgcttcatgt
      301 cacgtttgga aatatgacga agaaacagaa aatgcggaac gtatcgctat tttagacgtt
      361 aattacaatc aaataccgct tggtcaatca ggtgtatttt ctctcggttc aaacgctgaa
      421 aaacgttata taacatacaa ggtgtttgaa ggtgacgaac ttgttcacgc ggattattac
      481 accgttatcg acggtgatgc gtttgaatat gtaaacgcac cgcaggtact ttcggaaaca
      541 ggtgttatgg acttcagttc ggaatatttc nnnnnnnnnn gtgttacata tgaggacatt
      601 gcggaacgtt tgagcgatga ggacgaacat cagattgaag aagttcttaa aaatgtagta
      661 agctataccg attttgatat tgcaaactac actactatgg acgaatatca aaacgcactt
      721 aacgtcgaac cgaacggtga cagattttat ctgatttcag aggcatataa tcttggtgac
      781 gagatttatt acgttcgttt ctctaccgac cgttcatatt acaactatgc acttttgcgc
      841 agaagtgata tggctgataa cggttatcag attttgaagg tcagaacgga ctgtatacct
      901 ctttcggaca gagaacttaa acagataaaa gccgattacg acagaaacac tttactttat
      961 aaaaaatcaa aaggtactct gaagctttca aaaacatcaa agtatgatga cagcgacggt
     1021 ctttcagaac ctacaaaaga accgactatt cagattgaga aatcattaaa gagttcaacc
     1081 cgtctgccgg cagtatgctt gggcggcggt attgcaatag ccttgcttac agttttgtgg
     1141 gtttaccttt acagtgacaa caactaaaaa caaaaatccg atggtttgaa accatcggat
     1201 tttttgatta ttttttttct ttaagttgta caagctgtgc ttcgtatctt cttgacgtca
     1261 aattcgggaa cgctttaata atacgttttt ggaatatatg cgtttcttta acaagattat
     1321 cgtacttcgc tttaagttcg tccaaatcgt ccttatgctt tgacttgatt tcttctttct
     1381 tatgctcgta cttctctttc atatcgtcca aattctcacg aatttcctgt acattttcac
     1441 tgttgtatat ctcaatgccc tttttcataa tatcgttggc actgtcataa atatgttcgc
     1501 cgacttcgtc ggaaacatta tgaattttct ttgcaatatc attcataagt ttaacacgtt
     1561 ttgtaagatt attaatggtt ataacggtta taataacgtc agccacaaac actgccgcaa
     1621 aaaatacaac tgcaaccagt cccaacatat gcggtataaa tcttacaaat ccgagtataa
     1681 cagggtgaat acctttcata agtgtgatac acacaaaacc ccaatatatc gaaaatttaa
     1741 ggcatatgta accgcctatg ttaaacggca tatccgtata atcccaccaa cgtgcgtgga
     1801 atattttgtc gagtgcaaaa cctgtaatca gttccaatat tgacgtaagc aacgccgagc
     1861 ctacgaaaag taaaaataaa ttatctttta tcggcgtaag acataataca actatcacac
     1921 ctccgacacc gtatatcgga caaacaggtc cgtttaggaa accgcggttt ataaaatgac
     1981 cttcattcag tccgcaaaat accacttcga tnnnnnnnnn naatgcgtaa atgaagaaca
     2041 gccataaaag ttcataaatt gaatagccta atatcatttg tttgttcctt tcattaataa
     2101 taattatttg gtatctttgt tgatgtattc cgctttcaac tttgaataca tgatcttttg
     2161 actgctgtat aatccgtaat atcccgataa catataactc acgcctgccg caatggcaaa
     2221 aagtataaag ccttttgagc cgaacaattc aatacttaaa actatcgacg caatcggaca
     2281 gttaagcaca ccgcagaaca ttgcaatcat tccgatcgcc gccgcaaatc ccggatttaa
     2341 accgaacaaa tgaccgacaa cacaaccgaa tgtcgcaccg ataaagaatg tcggaacaat
     2401 ctctccgcct ttaaatcccg caccgattgt tattgcggtg aacacgattt tcaatgccca
     2461 cgcccaacct tttgcgtgac cgccgacaac ggcgtgttcg attatatcca ttcccgaacc
     2521 gttataatct cgtgagcctg ttaaatatgt aagtgcgatt atcgccaacg caccgacaac
     2581 tactcttata tatggatttt taaaccatat ttttaaatat cttgccgtat agtgcattgt
     2641 cgtacagaat attatgctta gttccgcaca caatgctgaa ataagtattg tctgtaaaat
     2701 tgttataacg cctgtttgcg gaataacggt gattgcgaac tgagtcggtt caagtcccgc
     2761 aaatcttgta accaaaa
//
LOCUS       DAWACS010000163         2775 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3814_length_2775_cov_2.225735, whole genome shotgun sequence.
ACCESSION   DAWACS010000163 DAWACS010000000
VERSION     DAWACS010000163.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3814_length_2775_cov_2.225735"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          789 a          483 c          623 g          880 t
ORIGIN      
        1 catctcgtgc tattcgtttg gcattgcttt aatcgtagat atatcggcac ttgtatgtaa
       61 tttgaatttt gcgggatttg agcatacgcc gatgcatgac acagccagta tttttgccaa
      121 caaaggcttt ttggggtatg ttgttgtagg aattttggcg tttttacttg gcatttcgct
      181 aactttgatg tgtgacattc ttcataaaag aaataaagag aggaatgagg gaaatgacag
      241 aactgacaga taatctttta cagcttttct gcgtttttat ctgcggatgc tgctcctgta
      301 tttcggcaat acgaaacaga agttacaatc gccttttggt attgcttttt tacctgagtt
      361 tcggaatggg tcttgcctat tgggtactgt accttatatt gttaggcact tctccgcttg
      421 tgttctgtgt ttcggaactg agctggacgg catcgtatat ttttcttact ttacgtcttt
      481 atgcagatgt accgaaagaa aaacataaaa agaaagcaat attttggatt ttaccgttgt
      541 tttctctggg tatgggtatt ttcttttgtt tgcgaggcag ttattttgag aacatcttaa
      601 tgggcacagc aatgggaata ttgggctttt atgcggtaaa aggtatatat tttgcaaaaa
      661 ttcaaaaaca gacgggtaag ctgtggattt ttgcggcggc tttgattttt tatgcggcag
      721 aatatctttt gtggggttct tcgtatttta ttgcggaaaa cacctttata aatccgtatt
      781 atttggttga catatttata atgaatccgg ctcttattct gatagccgtt gcacagagca
      841 gggaggaaaa gctatgccgt acaatataga aaatatattt atatgccttg cggctccgtt
      901 tttgcttgcc gcactcggta caaataccgg caacagaaaa aattacattt ttatagttct
      961 cggatttgtc tgctgtatta tatccgccta tttgaataca tttttttcgg tactttacgg
     1021 tgcacaggaa acatctgctg tattggaaat atcacctgtt atagaggaaa cggtaaaact
     1081 cattcctctt ttgtttggtt tgattgtttt tgaacttcaa ccgcatgaag gggataatat
     1141 tatttttaat attgcggtgg gatttgccac ctttgaaaat atctgttacc ttttggaaca
     1201 cggaacaggt aatttgtttt atttattagt gcgaggtttt tcaaccggag ctatgcacat
     1261 tacatgtgct tttataatcg ggtacggact tagagtattc cgccgacctg ctacgttaaa
     1321 gacatccggc atttttgcgc tgttgtgcgt cgcgattaca tttcacgcta tgtttaatat
     1381 gcttatgaaa actgaaggct taataaagca atttggatat gcggttccga ttgtttcttg
     1441 ccttattctg tttctgctga aataccgttt cagaaaaata actgatttaa aataactctg
     1501 ttttatgcag accggaaaaa atccggtctt ttttttgttt tttaggggga atttttatat
     1561 tttcaagcac ctatataata aaagggtgga ttgaggaggt gtgtgaattg aaatctaatg
     1621 cggaaagaat gaatttagtt ttcgcacgtg cgaaaaagat aaaacattgc agggagataa
     1681 aaaagctgaa tttctgcaaa gtggcaaccg ccctgctttc cgttcttttg ctgtgctgca
     1741 ccgcattatt tccgacatcg tacaaaagtg cggcggttgc cggttactgc ggtgcaattc
     1801 tgctgattga cggaatgtgc ggatatgtgc tgacagcagt tgtgactttt ataatagcaa
     1861 cggtgtttac cgtaatttgt atcaaaataa aaaacaggag ggacagaaaa tgagcaaaaa
     1921 aatagtgtcg gtgatattat cactttgcat catagtttgc gcgctgccga tgaatattct
     1981 tgcggcgccc gagcaaaacg aaacacttac ctttaacggc ggtaaaacgc ttcttctgca
     2041 aaacgattac ataagctttt atttctacga tttgcagtat caaacctata cggcaacggt
     2101 tccgagggca atcgcaaagg aaacaggcga ggtttttaca caggatattc aagccccaga
     2161 ctgccaactt aatgtttata ccggcggggg aaataagaag aaaattcatc cgtatgtcac
     2221 actgcaaaaa gcggagtttg tgtcagaaac accgaacgga aaaaataacg caattaaggc
     2281 agaatacaac atggaaatgg ttctgaacga tattcccgga actccgtacg gcacaataat
     2341 accggcaaaa gcaacggttt atcacgagct tgtatgtctt gacaaaaagg ataaaacggc
     2401 ttggggcgtt ttgacaaccg tcagtgaaat acaaatgaac agagatgatt tccccgaaca
     2461 tgatttgtac tttgagtggc ggtatgtgat gaacaatttt accggaatgg gtcacagtga
     2521 aaccgcaaac agctccggag gtccggcgat taagatggat cgcccaacag taaccgaaag
     2581 cggtgaaata acaacgaaaa gttcggttgt aacaggcaaa attgatgatg tgtcaacaaa
     2641 gcacgttcca aaagggtata ccttgtgggg cgatgtagac ggcgtttatg ttaatgaaat
     2701 ttacacagat gcatatccgt gggcaaatcc gtttgtcggt ttgtccgatt actacgataa
     2761 gttcgatatt gtgta
//
LOCUS       DAWACS010000164         2772 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3820_length_2772_cov_2.547295, whole genome shotgun sequence.
ACCESSION   DAWACS010000164 DAWACS010000000
VERSION     DAWACS010000164.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3820_length_2772_cov_2.547295"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          908 a          434 c          693 g          737 t
ORIGIN      
        1 gcggcagaag aagtaatggt ttcaagagat atactaatcg cagaagctac acatactgca
       61 attcacattg cgcatgtaag tacaagaggt tctgttgaac ttgtaagaca ggctaaaaaa
      121 cgcggagtaa acgttacttg cgaaacttgc ccgcattact ttacacttac tgaaaatgct
      181 gttgacggct ttaatacaaa tgcgaaaatg aatccgcctt taagaacttc tgatgatgtc
      241 gaggcaatca aagagggatt gaaagacgga acgattgatt gtatcgtaac agaccacgca
      301 ccgcatcata ttgatgagaa aaattgcgag tttgcacttg cattaaacgg tatcgtgggt
      361 tttgaaacat cattgggatt gggacttcaa taccttgtaa aatccggtgt tttgactttg
      421 aacgaactta ttgaaaaaat ggcagttaat ccgtcaagaa tactaagaat aaataaaggc
      481 agtttgtcaa tagacgcaga cgccgatatt acgatatttg atccgtcaaa agaatggacg
      541 gttgatattt caaaattcca gtcaaagagc aaaaactctc cgtttgacgg ctttactctt
      601 tgcggtaaac cggaatacgt tatagtaggc ggaaaaatag ttgtaaacaa tggtgaactt
      661 gcgtaggagg taaacaatgt cagtagattt attagtacag aaaattaagg aaaagggcaa
      721 tccgtcagta gccggacttg acccgaaaat cgaatatgtt ccggagtata tcagaaaaaa
      781 agcatatgcc gaatacggac agaatttaaa gggtgcgaca gaggctatat gggaatttaa
      841 caagggtctt atagacgcac tttatgacgt tgtacctgct gtaaagccac agtcggcttt
      901 ctatgaaatg tacggtctgt gcggtgagga agtaatgcac agaactattt cgtatgcaaa
      961 agaaaagggt ttgtatataa ttcttgacgt aaagagaaat gatataggca gtacggcaga
     1021 ggcttattca aaagcatatc tcggtaaagt tgatatagat ggtgtggaat gtgagccttg
     1081 ccctgttgac tgtgttacag taaatcctta tttgggaaca gacggaatac agccgtttgt
     1141 aaatgattgt aagacgtttg ataaggcgat atttgcactt gtaaagacat ctaatccgtc
     1201 atccggcgaa ttgcaggatt tgcttgtcgg tgacgaacat atatatgaaa aggttgcaca
     1261 gtgtgtgaat aagtggaacg aagaaactat cggcaagagc ggatacggtg cagtcggcgc
     1321 ggttgtcggt gcaacatatc ccgaacaggc aaaggttttg agaaagctta tgcctaaatc
     1381 atacttcctt gtaccgggtt acggtgcaca gggaggcggt gcagagggtg ttaagccatg
     1441 ctttaatgag gacggacttg gtgcgatagt gaactcgtca agaggtatta tgtgtgcata
     1501 taaaaagggc gattggaaag aagaacagtt tgccgaggcg gcaagagcag aggcaatcag
     1561 aatgaaaaat gatcttacaa gcgtgttata aggagtagca gatgaaaaaa gttgagaatt
     1621 gcaaactaat cagcaagacg gaaattatag acggaatttt tgattttgta atcgaaagtg
     1681 aagatatttc aaaagaggca caatgcggac agttccttca tataaattgc ggtgacagca
     1741 catttttaag acgtcctata agtatttgtg acgccgaaaa cggaaaagta agatttattt
     1801 ttgaagtcaa gggtaagggt acagaggaac ttgcaaaaaa agaagtcggc gattatatag
     1861 atgttatggg acctttggga cacggctttg aaataaagga cagtgtgaaa aatgctgtta
     1921 taatcggtgg cggtatcggt gtatttccac tatacaaatt ggcaaagaat ataaatgccg
     1981 atgtatttct cggtttcaga agtaaagaca gagttgttat ggaagatgaa tttaacgatg
     2041 tttcaaatat ggttatagtc ggaacagatg acggaagtta cggttacaac ggatatattg
     2101 catcggctat ggaccaatat cttgattcgc ataagtgcga tatgatttat tcatgcggtc
     2161 ctatgcctat gcttaaagct gttaagaaaa ttgcggaaaa cagaggtata aagtgtcagg
     2221 tgtctttgga acagcgtatg ggctgcggta tcggtgcttg ccttgtatgt agctgtgaaa
     2281 cgaataaaga gggtacagac agatatgcaa aggtttgtac aaacggtcct gttttctact
     2341 ctgaggaggt gactctgaat gactaataca aaaattgatt tttgcggtgt tgaatttaaa
     2401 aaccctatcg taactgcatc gggtactttt ggcttcggta tggaatacaa tgaatttgta
     2461 tctatcgagg agtacggagg gttcggtgca aaaggtctta caagagtacc gagagaggga
     2521 aacaaacctc cgagaattgc agaaacgccg agcggtatat taaacagtgt cggactgcaa
     2581 aatccgggtg ttgagcattt cgcaaagcac ataatgccga tacttgcgga atatgacaca
     2641 aatgtaattg caaatatgtc gggtaacacg atagaggaat attgtgaaat ggctgaaatt
     2701 ctttcagata ctgacgttgc aatcatagaa atgaatattt cttgccctaa tgtaaagcac
     2761 ggcggtttgg cg
//
LOCUS       DAWACS010000165         2763 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3845_length_2763_cov_1.960487, whole genome shotgun sequence.
ACCESSION   DAWACS010000165 DAWACS010000000
VERSION     DAWACS010000165.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3845_length_2763_cov_1.960487"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          761 a          448 c          654 g          900 t
ORIGIN      
        1 tacaaaaagc gtgaccttat aagaggagca ttgctgcacg attatttctt atatgattgg
       61 catgacggcg aaaaggagag aagaatacac ggttttactc atcctatgac ggctcttaga
      121 aatgctgaca gagattttga tttgaccgat acagaacgcg atattataaa aaagcatatg
      181 ttcccgctta ctgtaattcc gccgacttgc cgagaagggt ggatagtatg tttggcggat
      241 aaatattgtt ctctttatga aacatttaaa aaagaagcgt acagaaagat aaatacaaag
      301 attatggggg actatgctga attgtataag gagatacaaa tgatttaaat acgggggggg
      361 taaatgatga gtttttgcca atatttcttg tgctttgcgg tatttagctt tattggctgg
      421 gcttatgaaa cggtatatta cagcatacaa caaagaaaat ttgtaaacag cggcttttta
      481 agcacttgct tttgccctat atacggcatg ggagcaatgc ttgatttgat attgcttgga
      541 tggattgaaa atcctttgat actgtttttt gcgggaatgg tggttacctg ttcgcttgaa
      601 tattttgtat cgtggctttt ggaaacgctg tttcataaaa gatggtggga ttatacaggt
      661 tggcccggta atataaacgg acgtgtttgc cttataggcg gaattgcgtt cggtacattt
      721 accgttgcac ttattaagct tattgcaccg gtgacgattg atatgattga cagcgtgagt
      781 ttgccggcgt tacatattat gacgtgcgta attgcatttg tgatgattgc ggatacagtt
      841 aagacggtta agagtatgga ttcgtcaaaa ctttggtatg tggaaaaaca agccgaattt
      901 atggacgagc tgcgtaacgg cagatgtgtg gggttggtta aaaaaataaa agatacattt
      961 agaagataaa cggagcattt ttgctccgtt tttgtgtgca aaaaagaacc tttggcgcgg
     1021 gcagatggtg atatttcacc gtcaaagtcc gtatatgcct ccggttctgt tggtattata
     1081 taatataaaa ataatgctgt caataaaaag aagatgtaga ttgttgagaa gcgactgcta
     1141 caaacagtgt aaccacgcct gacacgtcca aaacgtccca cccactacat cttctatcgg
     1201 cagcaaatat tgtattgatt gtcaacagtg gatgtagggg cgatatattg gactatttcg
     1261 ttaccgtttt tgagtcagtg attggaacaa gattgcttag agaattccac cacataactt
     1321 ttacagtatt ggttgcagaa agcagcggaa cacttatatt ttcttgtggt gaattgatag
     1381 tacaacgtaa taatctgccg tccgaatgaa attcagaaat taagataatt ccgcctgaag
     1441 tgctgtttgt gagtttaaca gatatattgt tgccttcaat gttgcagtta atatcatgtg
     1501 taggacttgc tgtaggacac gttgtagcag ttgggcttgt tgcaggagtt attgtcggat
     1561 ttgtagtcgc agtcggacgt ggtgttggtt ttggggtaga agtcggactt agtgtcggac
     1621 tcgctgtcgg agttgcagta ggatttaatg taggttttgg agttgcagta gtcgttgctg
     1681 tcggagttat tgtcggggtc ggatgtggag tagctgtcgg actcgtggtc ggagttattg
     1741 tcggagtagc tgtcggactc gttgtatgag taggacgtgg agtcgcagta ggagtatgac
     1801 tcggaatagg actcggagtc aaagtcggag taggggttgt ttctgcaatt ccgctaattt
     1861 caaactctaa tttatctgct gacatatttg ttacataaac aaatatgtcg ttaccaatat
     1921 aaagttcttc gccgtcaccg actgcacaat ctgacatagt tgagtattga ccgttagaaa
     1981 caacttgcat attccagtta acgcctgtag tgtttatatt tgagggaaaa cgtacacctg
     2041 tgttagcaag atagcaaatc agtccgctct taagtgttac accttttatc aagtcaccgg
     2101 tattgcacga atagagtttc ttgttttttg tatcatattt gttactattg tcatcgaatt
     2161 gacgatattc caaataaaga gtttttccgt tcggcaaatc tcgtttatat gcaacaactt
     2221 cattttgagc tgaggcagga tagagtgtat atgtaccgtt tttatcaatg gttttaattt
     2281 gattattgtc aagccaaccg agcgattcgc gttctttaac ggaaatatac tgtccgatag
     2341 gggttagatg ttttgccatc agtgacatat aatacaccgc gcttgtttgg ttggagcggt
     2401 acaagtcttt caagccaagt gcgtgcatgg tttcgtgaca tattttacct gtcggtaaaa
     2461 taggcagatt atcctcaccg cgataaagca caagtccgtt tacgttttcg tagttgcacg
     2521 ttagctgtaa atattctccg ctctgataag tattaactcc ttcttgtact gaaatcatat
     2581 ttgaatatga gtgatagtcc caaagaggtg aattccaacc aaccgaaata ttttgcgttg
     2641 tatttttgta aatcacagta attaaatcaa tttttccgtc acggtttcgg tcaagatcgg
     2701 caaaattata ttgattttct tctatatctt ttggcttgtt tccgttcgtg attgcgtttg
     2761 aaa
//
LOCUS       DAWACS010000166         2759 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3857_length_2759_cov_2.748521, whole genome shotgun sequence.
ACCESSION   DAWACS010000166 DAWACS010000000
VERSION     DAWACS010000166.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3857_length_2759_cov_2.748521"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    365..374
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          657 a          633 c          482 g          977 t
ORIGIN      
        1 agatgtttaa tataaaatgt gtcgctttct aacattttat ccgcaagtat ttcacgctgc
       61 ctttgttcca taggaaattt tgtcctgtat ttcaaattat gcgctcttaa aaatgcgtct
      121 atttcaaaag caagttcaaa tacttcgtct aattcgtaat cctgagtagc accgtccggc
      181 tctctaaaat ctttttcatc tgccataggc taatcctcca tatcaatatc aggttcaagc
      241 tccagcttag aaaattcatc attcgtcata cgctccagct tgtcaataac cgtttccgta
      301 atttccttta attccgtatc ttcaatgtac ggaacagaaa aacggagctc ttttaaaagc
      361 tccgnnnnnn nnnngatgta tcataaatac acatcaaatt tatttcgtcc tgtgaaaatg
      421 tcatagtaaa atatcctcct tttgttgttg tgtttttgat ttcatcggtt cggatttacc
      481 cttgttttcc gcaagctgct ttttgattga tggctttttc gctgtccgtt ctgcaattat
      541 ggcattgtat aaatcaatat ctgcctgcgt tagtaaatct gcttcgccaa aatctttgct
      601 taattcgacg tgggttataa tatcctcctt ttcagcttcc gtcattttat caagctgcgc
      661 cttgattttc ttttttgaca aatccgcaat atgattgtca atattgccgc atttcagctt
      721 ttcaagtcgt ttatgctcgc acgatagagt aaattttaat tcttcatcag cggaaacact
      781 atcggcacac accgaaacat ttataacgtg tttaaaatgc ttcggataat ctaattcata
      841 aagattgccc ttgatgactt cattttcaaa acccgttatt ttaatagcat aggcaataat
      901 attgtcgtgt gcctgttcgc catagtatgt ccaagtatta tatgctgatg tatcttttgt
      961 aaagacgtcc gtttcacaat aacagtatgt accgttcaga cgcgacataa aaagtaaata
     1021 cttatcttct gcactgtcgc ttaacgctgc cgtatgtatt gtatcaacat caagaaaaaa
     1081 atcatttttg taatgctctg tatttatgtt cattattcgg cgcagcgaat ttataatatc
     1141 cacattttca aacttacgca tagcttaaac ctccaaagcc gcatttttga ccttttcgga
     1201 tttttgcggc tgtttatctg cctgcgcctt tgcttccgcc aactgctttt tgattgacgg
     1261 ctttttctta gatgtctcat ttttggaagg cgttttgcct ttcttaactt tttccgcttc
     1321 atttgccgcg ataatatcca taatagcgac aggttcgcca ttcttcgcct ttgcttcaag
     1381 ctcgtcaatg gtaggtgtgt ctttggaatt gtcctccggc tcaaatactt ttgcgccgtc
     1441 tgccgcgtct gcgtccgaca attcagaaaa tccgtcctgt tcggctatct tcgccggttc
     1501 ctcgtttcgt ttctgaaatt cagacggtaa tgtaccgtct aatggtctga tatactccac
     1561 cttgtcagca gcgttaagga cttcgcctgt taatggctgc tgccacgctt ttattttcgg
     1621 ctcccaatga aaaccgtttg cctttaattc ggcgcggata ttatcgtccg gcttttcatt
     1681 aaaaaatact tgcagataat tagcgtctgt attgattgca gcgtatccgc cgttaaaatc
     1741 ccagcccgta aattgtgtat aatccgtttc ctgttcggtc tgctcaatat cctttgcaag
     1801 ctctttataa tactcgtcaa tgcctaatat aatttcattc gccgtttttt gtattgtgtc
     1861 aagggaagcg tttaactccg gtaattcctt atctttgctc caacccgcaa catatccgaa
     1921 agaataatcg gaagtgtcaa tcccatatcg ctgacaaacg gtataggcga cgctttcagc
     1981 ttcaacctcc attgtacgat tgttcttttg ttcttcgggc gtcaattccg ttgatttttc
     2041 tttgagaata ttgtgtagtt tagcgtgtgc aatttcgtgt atcagcgttt ttaactgctg
     2101 aacctcgctc ataccgtttt ttacggctat tcgctgttct tcaaaattac aatatccctt
     2161 tgctcccgta tctatatcct catacccaat aggcataggc gatatttttt caagtgcggc
     2221 ataaaacttt ttgaaatccg caacattgcc cgtaagctcg tcaactccca aactcggcag
     2281 cggttcgccc tccgtctgcg acaagtcaaa tacggtagca actcggaaag tggggatagt
     2341 tacttctttt tcctctgtaa gcggtttacc gtcccagcct ataatcggct tgtttgttgc
     2401 cggatttatt ttctcaatct gcttttttgt tttgtatgag gacggcgcga ttattttaat
     2461 accgcgttca cctcgtttta ctgtgcgttt gtgattatcg cgccatgcgc caaatcccgc
     2521 caccatagaa gcgtcgggct tttgcattaa aatcaaaagc gaatttctaa aactgtaatt
     2581 atggaatttt gacatacacg caagatattc cgcatattta tcgccgttaa atatgtcttt
     2641 cactccgtcc tccagctttt tcaaaatctc gctcaatttt tcatagggcg tttcgccgga
     2701 taattctatc gcctgtacag aagcccttga cggctccgat ttttcaggcg cttttgttg
//
LOCUS       DAWACS010000167         2746 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3876_length_2746_cov_2.331104, whole genome shotgun sequence.
ACCESSION   DAWACS010000167 DAWACS010000000
VERSION     DAWACS010000167.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3876_length_2746_cov_2.331104"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    649..658
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2371..2380
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          724 a          586 c          422 g          994 t
ORIGIN      
        1 ttaatgtaca gttaccgtct attgttatat caacatttcc cataatattt atcggagtag
       61 ccgaattaga catttttatg tcaagattct tcaatgtaac aggcggaagt ttcgttgccg
      121 aatcaaggtt aactatattt tctgttgttg ttcccgttat tacatatcct aaatcgttgg
      181 ttttatattg tacaccgcca taggttacat aaagatcagt tacataaaca ctgccatttg
      241 aaacatcata aacatcggct tgcttttcgg tatgttctga atccaaagta agatttacag
      301 tatatgtcac ttcactctta tcgttacccg ttattttata cgttgctgat gttgtattat
      361 taaatttcat tgcatctttt tgagtaaatg caccgataaa ttctatgtta ggtgtaatat
      421 ttttcaatat gatgttataa ggtactttta cagagattac tctgttctca ttatctataa
      481 cagtatcccc gatttgaccc ggaattttaa aagaagaaac acttatctct gtattgtttg
      541 tacaattaaa tgtattcttt gacatcgaat caacttttga atatctgtaa tatattgtac
      601 cgtttttata tacacgaatc cattgatttc cggcatttgc atacaaatnn nnnnnnnnct
      661 tttctgcacc tgtatcatta tcagaaacaa gtgagtattc aacatcttgc tcatctgtta
      721 ttccgtcaac cgttgtttct accaaatact gattatactt agttgcagga attgttccgg
      781 aattattaaa cgcatcaaat gatgggtcat tatttggtgt tgttccgaga gttgcgtcta
      841 cacttccgcc attaattgta atagcgccgt acggagtctt atttccaccg ttaccgatgc
      901 cgtaacctga gccgttaccg acagctttaa cataaccgcc gtttacaatg acatttccgc
      961 ctcggccgct tgttcccgat gcactgcctg taccgcctcc gatacctgcc gcattgcttt
     1021 cagaatcatc atcggcatat gcgtaaacat taccgccgtt tataagtacg ttgatacccg
     1081 aaccgtcctt accgcctccg ataccggcac cgccatttcc gccgtaagca tatacagtac
     1141 cgctgtttac agtaaattgt ttcatatcgc ctataccggc gccgtttgca gacttaaatg
     1201 ttataacacc gtcattatca cccgaatctt caacgataag ttttgaagta gccgttatac
     1261 ctcttaccgc cgcaagattt acagattgcg attcaatttt atttgtgcct ttcagtctaa
     1321 gtgtcagttc aacatcgtca cctatcgtca atgcattgtt tatagatgaa cgagataaaa
     1381 tattaatatt atctaaaaca atggtaggat ttgcaccctg taccacctca attacattac
     1441 ttgtttgact tgttgattga actatatata tttccatatt ctttggaata tcatagcttg
     1501 ttgtaccaac cgtaactgtt actatatcgt tattatcatt gttttttgtt attttgatat
     1561 ttccttttga aatatcaaac tgcgatttat catcacccgt caaagaaata tcttttgaac
     1621 cattaaaaat atatccattt aaagttgctg ttaccttaac acgaatttta tgagttccct
     1681 gcttataatg tgcaactata tatccgtcca caacttctgc caaatcactg cttattgctg
     1741 tttcattttc gtcagctgtt gttaatgtat atgtacaatt aagaatatga gtaggataag
     1801 tacagtcact tgatttttca aaatcagttg ttaatttgat ttttgtctgt ccgacaattt
     1861 tatttacagt tatttcatca ggtacagata atttaattga tgaatttgca tctgtacatg
     1921 tacagtctga acccgaatat tctgtcagtg ttgtttggtt ttctatatcg tctgtacgat
     1981 atattttacc ggcatatgtt acggcatagc ctgttgtatc ttctgttaca tatacataca
     2041 gctttgcttc gctgtctgtt actgttttcc atgagccgac agtaacttct ttatttgcca
     2101 tttcaactgc atccggcatg gtcagaacta cttgtttaag tttatttcca tacttatctt
     2161 gaactttgtc ataatctgtg cttctgaacg taccatctac actgccgccg tttatgtata
     2221 tattatttac accaaattgt tctgttgtgg gagtcattct tcccggtcct atggaataag
     2281 tcaccgaagc accggctgtt gctttaatat atccgccatt tacgataatc tcctcattag
     2341 actgacgctc cacaccgcct atggctgttc nnnnnnnnnn ctgttccgta tcgtcctgca
     2401 cttgcattta tgataccgcc gtttatttct attgtacatc cgctgacatt accagatgtt
     2461 tttacacctc cgcctatagc tgtacctttc attcctccga cagcattaat agtaccgccg
     2521 tttattataa ttttaccatg taattgcttt agattcgcac cgattcccga tgcttgtgca
     2581 gtacctgtta cattaagaat accatcaccg tcaacagtca aaatcgaata atccgactct
     2641 atactatttg ctgccacatt aatacctgcc ttaccgcttt ttttagcaat aacggtattt
     2701 gtgccgttta tgtgcaaatt aacaactgca ccggattcaa ttataa
//
LOCUS       DAWACS010000168         2732 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3905_length_2732_cov_2.395966, whole genome shotgun sequence.
ACCESSION   DAWACS010000168 DAWACS010000000
VERSION     DAWACS010000168.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3905_length_2732_cov_2.395966"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          709 a          692 c          419 g          912 t
ORIGIN      
        1 gaatatacgt ttaccgtctt aacaccgaca aagtcgtaat cattgtttac cgccgccgaa
       61 gtcatagact cccttgcgaa tttttcatca attttttgag catacttact tgcataattt
      121 atagccattg tattcatcct ttcataatca cgttatttat tacattccca aaccgcttaa
      181 aaacgggtct gttaaatttt ctttttctct tgaaattctc ggcggaacac cctttaatct
      241 ttcggataaa ccgctttcga ttgctttcat atattctgct ttaaatcgct cgatattttc
      301 cgccataatg tccttgtctg catctgcgac cattctcgcg aaattcacag gcagattttg
      361 agccgccaat tcctttgccg cctcaaattc ggctctttcg gatacatact gctgtctttc
      421 attttcaaaa tccttttgtc ttttgtccat ttcagccttt gctctgtcct ctgccgacat
      481 tgtagccatc ttcgcggcgt cctctctttc cgatacgatt ctgtctgaaa aatctttttc
      541 ccacgttgct ttttcgtttt taatcgcctc cgcaacatag cctgcaatga gatttttcag
      601 ttcatcgttt ttgtgtcccc cgttttcgtt tttcgtgtca ccgtttagtg tcccctgctt
      661 cttcttttcg tccatacttt tttcctttct ttacattaaa ataaatcact atgtttttta
      721 ttcttgaatt attcctgtac atattttaat ttttcgccaa gcattttttc ttttgcggca
      781 agctgtgcct cttccttcgg atcgcccaca aatgaaagct gtgataaaag agtttcgctt
      841 gacgcaatac cgactaagtt gtttaccatc tgtgaaatct ccaactcatt aacaggcaaa
      901 tttctcgtaa acacaacatc aatgcgatgt atcggcacat actccatagc accttttaac
      961 gacagaaaat taacatacaa ttcaaatctc tgttttaaag ccttcgtaaa gtatcgctcc
     1021 ttgtttctga cgtgctgttc aaaccccata agcttatact taatcgcgac acccgataaa
     1081 tttgtaccga acgtctggtc tgataaatcg ggaatcatag aaaatctgtg aatatcactt
     1141 ttcagactat cctttaacac ttcaatatcg ctttcggaca taacctttga aagatattga
     1201 gccgccgcac cctcatatcc catcaaaatg cgttcttccc taagtttctt cgcctgctca
     1261 ctgtcaaggt cgataccggt aaggaataaa aatgcgtcaa caaactgttc cttgtcgtta
     1321 actctgtccg actccaaaat attgtaagcg tcaatcagcg gaatcatctg ttcaaaatca
     1381 ccttgctttt cttcgttgtt gcgatactcc accataggca ttttaccgaa atagtggtgc
     1441 tgagaatttg tcatagtcat atgcaaaaaa ctgtccgcag tgttttcata tgtataaatc
     1501 atctccctgt cacacacaat acagcaaaca cccgtaaccg caccgtcaat gtcacgcttt
     1561 ttgtaataat gtatgccgta cagcgggatt tcgccaacgc ccgcaccgta cacaataaac
     1621 gtgttgctcg gcggcaagca tacacttttc ggctgtgatt gctcgtccgc ataaaccagt
     1681 tcataagctt taccgaaaat gctcattgtc ttttcaaatt ccgcgtccaa actcggcata
     1741 tcctgaacgg caaagcaatt tttaaccgcc tcaatatcgt atccgtccga acagctgtac
     1801 gccacaggat tgcctacaag atagcttttt gcaatatcca cgatatactt cgcgtgattg
     1861 cacacaattt tgttgttcgc gatattgtcg cttgaacgtc tgcgttcaca aattgcgtgt
     1921 ttgccgatat aatagttgta aagtttttcg tatcgctcct gttcaagtga atgtttccca
     1981 atcagcttag caagcaacac ctcgtctata ccgtccttta aaatatcctc atcaattatc
     2041 ataaatcaag ttcactcctt ttaattaccc tcgctttttt ctttgtgatt tcgtcctcaa
     2101 gtgcataacg caccgcatca atggtgtgat tgttcttgtc gggataaacc gacttaaatt
     2161 caccgtccgt atcgcgttca agctcatatt ccgaaaattc tcttgcgaca ttcggacacc
     2221 tttcgggatc aatcacgatt ttttcaagac tctgcaaaaa tctgataccg tactcgatac
     2281 tgtccggacc tttccttgca ccgcggacac gcaaaccgaa cattcgcaaa tcattgatgc
     2341 ttttcggctc ggcactgtcg catacaatca atttattagc aaaccctttt tcaaaaatct
     2401 tttcagccgc attgtgattt gtcatccctt tcgcgtaaat ttcgtcaaag ataaacaact
     2461 tattgttgtg ataaaaacac accacataca caaacggatc tgccgcgaaa ccgaaatcga
     2521 caccgcattt aatattgtcg aagcttttta cttcctcatc gctaagtttt acaacttcaa
     2581 gattatcaaa aatttcaccg cctgttcccg tcacttcacc gagatattcg tgattatact
     2641 gattaatatg tagcttttta agatacttcg cttccaaaaa aaattgttct cccaaccagc
     2701 ttttcggtac atcaagatat gtactcttac gc
//
LOCUS       DAWACS010000169         2728 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3908_length_2728_cov_1.758698, whole genome shotgun sequence.
ACCESSION   DAWACS010000169 DAWACS010000000
VERSION     DAWACS010000169.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3908_length_2728_cov_1.758698"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          795 a          568 c          442 g          923 t
ORIGIN      
        1 gtactaaatc aataacctca atgattatat actatcgtta ataactttgc aatattttct
       61 tgcaaataaa cataaacatt gtacaatatg tttaaatata ttgttctaat atgttcataa
      121 ttacccaata gatacaaaat attgtgctgt gcagtctgtt taacacctgc tactaacacc
      181 atattgtatc cgtaaatcat cattttttta tgattataaa cacaaaaaca gacaaaacaa
      241 taatgttttg tctgttttta tattgtattt tatgggatta tttatctgaa ttgtcacatt
      301 tcttgtaaca gaagtcacaa ttattttcaa gcggtgcttc accctttttg cttctgtaat
      361 cgttgattgc agactgaata gcctcttcag caagaacaga gcagtgaatt ttttctttcg
      421 gaagtccgtc aagtgcctcc ataaccgctt tatttgtaag agcaagtgcc tcgtcaacag
      481 tcttaccctt aaccatttca gttgccattg aacttgttgc aatagccgca ccgcagccga
      541 atgtcttgaa cttaacgtct tttataacat cgtcttgaat atccatatat atcttcataa
      601 tatcaccgca ctttgcattg ccgacttcac cgactgcatt tgcgttgtct atctgaccta
      661 catttcttgg atttgagaaa tggtccataa ctttttcact gtacataatt aattatctcc
      721 tttctctaca ccctcatata aaggtgacat atctcttaat ctttgtataa tcggaggaag
      781 tacctctact acgtagtcaa catcctcctc tgttgtttca tcgcctacac tgagtcttag
      841 cgaaccgtga gcaatttcgt gaggtaaacc gattgcaata agaacgtgtg acggatcaag
      901 cgagcctgac gcacaagcac ttccgcttga cgccgcaacg cctttcatat caagcatcat
      961 caatattgat tcgccctcaa catactggaa tgagaagttt acgttattgc aaagtctgcc
     1021 ctcgtcctca ggaccattaa gacggcaata aggaatattc tttctgatac cctcaattaa
     1081 tctgttacgc attttctttg cgtgttcgat attttcatca aggttttccg ttgcaagctc
     1141 aagtgccttt gcaagacctg ctatacctgc aagattttcc gttgctgctc tctttcctct
     1201 ttcctgtcct ccgccgtgaa taaggttgtc tattcttata ccgtttctga tgtacaacat
     1261 tccgacaccc ttaggaccat ggaacttatg tgcagaaagt gaaagcaagt caacgcctaa
     1321 atcctgcaaa tctatcttca tatgtccgat agcctgtact gcgtccgtat ggaaaataac
     1381 tctgtgtttc tttgcaattt ccgcaatttc cttaattggc tcaactgttc cgatttcatt
     1441 gttagccgtc attattgaaa taagtattgt attgtccttt atagcgtcct caacagcttt
     1501 cggacttaca aaaccgtatt cgtcaacatc aagataagta acttcaaagc catgcttttc
     1561 aagatattcg caagtgctta aaattgcgtg atgttcgatt ttgcttgtta taatgtggtt
     1621 gcccttcgac ttgtttgcaa gtgcaatacc ttttatcgcc cagttgtcag cctcgcaacc
     1681 ggaacccgta aaataaattt cgtttacgtt ttttgcatta agactttttg caacgctctc
     1741 tctaagctta taaagtgcct gttcggcatc tctgcctatt ttatagaatt tcgatgacgc
     1801 attgccgtat atatctgtat aatatggcat cattgcatta agaacttctt ttttaaggct
     1861 tgtagtagca ttgttgtcca tgtatactga attcattaaa aacatctcct gtattaaaat
     1921 tgaacatttg aataactgtt caaacatatt ttactttata tttcctactt tgtcaatagg
     1981 ttttgtagaa tttctttctt ttattctata caaaatttat gtctgtttta catcattgct
     2041 ttatttaata ttccaatttt atttattgct atacttctta ttttgagata taatatatat
     2101 taacaatgta attttgagga gataaaaatg aacattaccg gtattataac agaatacaat
     2161 atttttcaca acggacacaa atatcaaatt gacgaaataa aaaaacattc ggacgcagtg
     2221 attgccgtaa tgagcggcag ttttgtacag cgtggcgacg ttgcgataac ggataaatgg
     2281 tcacgtgcaa aaacggcact ttcgggcggt gttaaccttg ttattgaact tcctgtatgt
     2341 tatgcgttaa atgccgcacc gaattttgcg acaggcggta ttaatatttt aaacgcactc
     2401 ggtgttatca acaacatttg tttcggctcc gaatcaggca gtatcgatga acttatgtct
     2461 gccgcagaat tgcttgaaaa cgaaaacagc gaaatatccg aaaaaataaa aaaatacatt
     2521 atgggtggta tgagttatcc aaacgcgctc acaaaggcat attccgcact tatcccgtca
     2581 gatatattgt ctgagccaaa caacattctt gcaacggaat atattcgtgc attaatccgc
     2641 tctgacagca aaatcaaacc tatgaccgta aaacgtcaca aagcaggcta ccacgacaga
     2701 aatttgtatc aaaatatagc aagtgcga
//
LOCUS       DAWACS010000170         2724 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3914_length_2724_cov_2.580367, whole genome shotgun sequence.
ACCESSION   DAWACS010000170 DAWACS010000000
VERSION     DAWACS010000170.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3914_length_2724_cov_2.580367"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1311..1320
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          822 a          511 c          709 g          672 t
ORIGIN      
        1 gccttcagcc ttgaactgaa tgcctttgac ctgtttgaaa ccgaggcggc acttgctctt
       61 gaacgtagca ataacggcgc tcttatccct gacgagttat ggttttatgt aaaatcatca
      121 ccgggaattg tacttgttcc gcctgttccg ataggacagc ttaacggcgg cggtgccggt
      181 tttaaggatc ttgccgcaac ggtaaatggc aactatcttg ccattcctcc tattaaatta
      241 cgcggtgcat tgacgggaac atatttgcat cttatcgaag gaacaggtaa cattattata
      301 ggaccgagcg agatttcact aaaagcgacc gatgttaatc ttgtaggtgc aggggcggca
      361 acgcagattg tcgacagctt cggctattcc ttaaaattaa acggacagga acgcagttat
      421 aaaggtaata catatgaagg catttatttc ggcggctcaa aggagcttgc attaaatctg
      481 ccgagtaaac agatagacgt tataacgttt gattcttcta ttgaattggg tgctttcggc
      541 ggagtgaata acagcaagga ttatctgtat cttgcaatcg gtgcaaacgg tacggttgca
      601 ggcagagttc agattccgaa aagtaggcac gtccttgccg gcaaagggtt taacgtcgga
      661 aatattaatc tgatcgtagg cggtcagacg gctttcccaa tcagaaacgt cacagttgag
      721 gagggtatga agcaagcctt ccaaaatgtc gatgtgtacc ttggcgtggt ggctgaggtg
      781 ggcggttggt tagccagcgc gcgagcatgg gtacttgtgc cgaaaatcgt agagaccgac
      841 ttccgtaaag gcggcggctg ggatattgag tttaagatgt taggttatat gcctgaatgg
      901 aactgggcgg ataaaggtgt gtcaccggtt gtaagtatgc ttgcggaaga cagcgatgcg
      961 aattttgcgg cggtgcgaga tgagaacttt gtgcttgcaa acaccggcgt gagtcggacg
     1021 gaaatctcag caagtgccgg tacagacgaa gcaccgtata tcgtacttgc ctttgacgga
     1081 aatctaaccg aggagcagat caaagagaac ctaaagattt tcaatgacag aaacgttgag
     1141 cttaatatcg actggatgac tgatgacagt cagtttaatc ctgacgaaga tgtcagcgcg
     1201 acaactattg cagatatgga aaaaacaaac gctgacggca aaaaatatcg tctggctctt
     1261 ctgcgactta aagaaggtgg caagtatatc gttgaggcag gcaagttgac nnnnnnnnnn
     1321 tttgagaagt tggcactgac actgaatgac aatcaaattt ccggtaaggt gaagtatgcc
     1381 gtggataaca cgccttacgt tatccgcact tatttttcaa acgaagaagg cggagcggac
     1441 tatatgattt cagaacagga aatcagtgat acctcaaata ttgaactttc cgttcctgca
     1501 tcgggtgcga tagcaccgac aggtgaatat tatgtaacag cctttcttat gacgaaaaag
     1561 caagcagatc ttaacggtga cggtaaggag gaagacgcac ttattgctat tgataatcaa
     1621 gcgtttaaca caaaagttgc atataccaat gtaaacgaac cgtccgcacc gacagatgtt
     1681 actctgcaaa catcaggaaa tgaggtaatg cgtgcggagt ggaatgcgtc agacaatgtt
     1741 gacggttata gtgtgagaat ttatgaggaa aaagacggag aatggacaga cacaggattt
     1801 ggatatgatt tggataaaga cacgacagcg gttgatatgg cactaactgt cggaggaaac
     1861 ggcgttcgtg taaatgaaaa cggagatacc gcggagtctg ttcctgccga gaatttgttg
     1921 cctgataaga catataaagt cggtgttcgt gcatacaaaa aatcggaaaa cggaaagtat
     1981 tacagtaagg aaaccgaatc tacaggcgaa tttcttccta aatatacacc tatggatata
     2041 gcactttcga tgaacggtaa cgaatgcact gccgatgaaa acggcgttta tcacgcgtat
     2101 atcggcggcg gtgacaatac tttgtctgta agcggatcgg acgctgatgc gacatttaaa
     2161 ctgacaagaa tggacacaaa tgaccaaata ccaaacgaaa acggtgaaaa cacctttgca
     2221 attccggaat ttgagggaag tttgatgttc aagattgacg gtatttccgg caaggatgta
     2281 acaagtgtat tcctacttat taatatggat aaagaaccgc ctgttctgac gctttcatct
     2341 gatatatttt atgcagacaa tgaaagcggc gactatacga taacaggaat atcagatgcg
     2401 ggtagcagaa ttatgtacgg cgataatgaa gaagttgttg caggcagtga cggtaagttt
     2461 gctgtttcgg gaaaactcta tgaaagtcaa acaagcggtg ctattatgct ttgtgcacag
     2521 gattttgccg aaaatacatc aataccgcag actgcacttg ttataaagaa aatatcaaac
     2581 accgtaacgg taaatgacag ctatgcggag aattcgggta gcggagagta ttcagagggt
     2641 gaaacggtaa cgattaaggc cggcgaacgc agcggatata aattcagcgg ttggacgact
     2701 gacgacggtg tacagtttgc ggac
//
LOCUS       DAWACS010000171         2712 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3945_length_2712_cov_1.984193, whole genome shotgun sequence.
ACCESSION   DAWACS010000171 DAWACS010000000
VERSION     DAWACS010000171.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3945_length_2712_cov_1.984193"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2440..2449
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          934 a          442 c          627 g          699 t
ORIGIN      
        1 taaacacgct tggatattta aaagaaaaat atccgaataa cgatttgttt tttataatcg
       61 gtgaagattc gcttaatgat tttgataagt ggtacaagcc cgataaaata cttgaaatgt
      121 gttcgctgct tgtatttccg agaaaatcgc taaagtcgct tacggaaacg ataaaggtcg
      181 taaaagaaaa aatgaacggt cggatatttc ctatatcagc acccgttttt agaatatctt
      241 caacggatat aagaaacaga gtgaaaaacg gtcagacggt aagatatatg ctgcctgaaa
      301 atgttagaga ttatataaat aagtatcatc tttataagga aaacgaaaat gatagataat
      361 tacaaggata aactaaagag tacgctaagc gaaaaacgat ataagcacag ccttggagtg
      421 tgtgacgaag cggtaaagct tgccgaaaag tacggtgcag ataccgaaaa agcatacaca
      481 gccggaattt tgcacgattg tgcaaagtgt tatgacacag acgaacagat agaactttgt
      541 caaaagtacg gtgttgtgct tgatgatata acgcttgcgt gtaagcctgt cattcatgca
      601 ccgctcggtg caaaaattgc aaaggctgag tatggagtga acgacaaaga cgtgcttgac
      661 gcgataatgt gccacacggt cgcaaagaag aatatgtcgc ttttggataa gataatctat
      721 attgcggata tgattgagcc tatgcgtgat tttgacggag tggaggagct taggaaagcg
      781 gcgtacgaaa atattgacag ggcatttatt cttggactta aacaaagcat tgtctttaat
      841 gcacagaaaa ataaaataat acaccccaat acattatcgg catggaatta tatgctgatg
      901 aacggaaaat aaaaggaggg acactaaatg actaaggttg aaattattca aaaggcactt
      961 gaagataaga aagcgacaaa tatagaagtg cttgacgttg cagagcagac atcacttgcg
     1021 gattacttcg ttgttgcgtc ttgtcagtca acggtacagg taagagcgtg tgttgatgaa
     1081 attgaagaaa agcttgaaga ggcaggcttt gagataaatc ataaagaggg ttacagaggc
     1141 ggcagctgga ttttggttga ctgcggtgac gtaatcgttc acgttatgca gcacgaaatg
     1201 cgtgaatttt atgctattga acgtctttgg gacgacgtcg gtaacgtaga cgaatcagaa
     1261 gaagaataaa ttttaaagga gcaaatatac aaatggagag ttacaacttt aaagctattg
     1321 aaaaaaaatg gcaggataag tgggaagaaa caggtgcatt ccacgctgaa acaaacagca
     1381 aaaagcctaa gtattataca atgatagagt tcccgtatcc gtcaggtgcg ggcttgcacg
     1441 taggtcaccc gagaagttat acggcacttg atattatcgc aagaaagcgt cgtatggagg
     1501 gttacaacgt actttatccg ataggctggg acgcattcgg acttccgact gaaaactttg
     1561 ctattaaaaa caaggtacac ccgaaaatcg taacggcaaa gaatattgct aactttacac
     1621 gtcagcttaa aatgcttggt ttctcattcg actggtcaag agaaatcaac acaaccgatc
     1681 cgagctatta taaatggaca cagtggatat tcttacagct ttttaagcac ggacttgcat
     1741 ataagcagga aatgccgatt aactggtgta ccggctgtaa agtaggactt tcaaacgaag
     1801 aagttgtaaa cggtgtttgc gaaagatgcg gaagtgaagt tgttcaaaag agaaagagcc
     1861 aatggatgct taaaatcacc gagtacgcac aacgtcttat cgatgacctt gacgatgtaa
     1921 attatcttga aaagattaaa acacagcaga aaaactggat aggcagaagt gagggcgcag
     1981 aggttaagtt taaactttca acaggcgatg aaatgattgt ttacacaacg cgtgcagata
     2041 cacttttcgg tgcaacttat acagttatgt cacctgaaca tccgcttatc gaaaagatga
     2101 aagattcaat tacaaattat gacgaagtgc ttgcatataa gacagaggcg gcaaagaagt
     2161 ctgaatttga aagaacagag cttgcaaagg aaaagacagg tgttaaactt gagggaattt
     2221 atgcggtaaa tcctgcaaac ggtgcaaaac ttcctgtatt tatttccgta tttccacttt
     2281 gtttcgaaaa aaaagcatat ctttgtcata cgctttctgt taatcagcgt ggtgaaaaca
     2341 ggtaagaagc gttatttatt ttgaaatagg aatattattg cattatccct cgtccttgaa
     2401 atctagcaag tttctgtccg tagaggggtg gttcaacagn nnnnnnnnnc tgcacacgac
     2461 agccgtgact gggatttcgc aaagaaattt aatcttccta ttattgaagt tgtttcaggc
     2521 ggtaagaacg tacaagaaga ggcttataca gatgtataca agggtaatat ggtaaattcc
     2581 gagttcctaa acggtcttcc ggtaaaagag gctattccta caatgattga atggcttgaa
     2641 aagcaaggtc ttggaaagag aaaagtaaac ttcaaacttc gtgactgggt attctcacgt
     2701 cagagatact gg
//
LOCUS       DAWACS010000172         2703 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3956_length_2703_cov_2.784366, whole genome shotgun sequence.
ACCESSION   DAWACS010000172 DAWACS010000000
VERSION     DAWACS010000172.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3956_length_2703_cov_2.784366"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          824 a          650 c          445 g          784 t
ORIGIN      
        1 attctgtaaa ggaagttgtc cacttgacaa agtgccgctt cctttaattt atcgtctttg
       61 ttactctcat aagcaagcca caattcaccg gtccaaaaac cctatgtcca gtctgtattt
      121 tctgtttgcc agtaaaaatt attttcactg tttgcacccg gaaaacagtc aaaaaactcg
      181 tcaacattat gttcaataag cgaaaccgcc ttattgtgtg cgtcacgaat ttctttatca
      241 tcagccaccg gatagccttg taattcttcc atagttatca tacttaaaat cacactcctt
      301 atataataat tataccaaca cggccaagtt atttcaatag caataattta atgtcaataa
      361 attgaatgta taacaatagg caagccgaaa aatataggct tgcctattca gtctacctct
      421 ttaattatcc tttaactccc gaagatgtag cgttttcaat atatgagaat agtgtttgac
      481 tcggcaacat caacgttcca agtatacaaa gacgattcac tctctcctgc aaagaccaca
      541 ggtacattgc aatccggtgc atctgccgac acattctcaa tgacaaactg gggtaaggct
      601 aacaacggtg catatttgag cggtgttcgt ttcacggcaa acaaggatat gaccccggcc
      661 gattttgtaa agattaaatc gctaaagctg tacgaaattg aaagaatggc tgatacagcc
      721 gttgacagtg ccgtagcggc aatcacaatc gataaactta caagcacacc ggaatgtgta
      781 gagcaaaaca tcactttgcc gcaaatcact tcagacgacg cagaagtaac atggacatct
      841 tccgatacat cggttatcgg caatgacggt acattctacg gttcatcaaa agcgacagac
      901 gttacaatga cggcacaaat cacaaataaa acagacagct ttacagttta taaagacttc
      961 cgtctttctg ttccgggtga agaaacagta aaattgagca aaacttttga cgacaacggt
     1021 atgaacgtaa cagttaagaa caattcttcg gacagcttaa ctatacaggt tacagtcggc
     1081 gtatacaacg acaatgatac tcttaacacg gctaaacttc aaaccgttac acttgattca
     1141 aaagcagaac aaacaatttc atttgcaggt atcacctccg acaaatcagt ttcaatattt
     1201 gcatgggaca aggatatgac accacttaca aatgtattgc aataacacaa aaaatacacc
     1261 tatcattcga taggtgtatt tttcatttgt ttttcaatcc aagccataag caaattcact
     1321 attttttctt gttggacaag agtaagttca acatttttcg gtattctgta ccacttgtta
     1381 aggcaactgc ggcagcagca cgccgttgcg tgttgtgcaa taaacacagg gtgtccgcgc
     1441 atgggtgttt gcttgccgtc atttgcaata aatgacggtg caagtctgtc acgaacaaag
     1501 tcgcgtgcgt gtgaacgcac agtatccata cctttatttt ttacatactc cctatccttt
     1561 tctttcagct taaaactact tctgaacttt gacttttcca actttaaaag tgcctgttca
     1621 atcgtctgca taaaatcact gtctttcata tcttccgtat gtacaggtta tatcggcaat
     1681 tttctttgcg agtttgtcag tcaaatcatt cttacccatt ctccactgcc agttgccgcc
     1741 gagtacagac ggtgtattca ttcttgcctc actgccgagt tcaagataat cctgcatttg
     1801 aattatcgcc gtatcgctca cacttgccat agcggtttta atgagtttcc aaacaatatc
     1861 atcttccgaa tcaatgtact ttttacaaaa ttcaagtgta tcttcgtcaa gctcatcaat
     1921 ccaaccaagc actgtattat tatcgtgcgt gccgacatag acaacactgt ttgaattata
     1981 tgtatacggc aaataattac tttcttcacg cggatcaaat gcaaattcaa gcactttcat
     2041 acccggaaaa cccgaatccc tcaacagctt atgcacgtcc tccgtcaaaa atcccaaatc
     2101 ctccgctata atcggcaaat cgcccaattc atttttaacg gcattgaaca attcaattcc
     2161 cggacctttc agccactgac cgttttcggc ggttttatcc ccgtaaggaa tcgaataata
     2221 cccctcaaat cctctgaaat ggtcgatacg aacaacatca tacaatgacg ttgcactctt
     2281 tattctgtct atccaccaat taaagccgca ttctctgtgc ctgtcccaat tatacagcgg
     2341 atttccccaa agctgacctg tcggagaaaa tgcgtccggc ggacaacctg caacagcttt
     2401 cggcacaaga ttttcatcaa gttcaaataa atcgggatat acccacactt ccgcactgtc
     2461 aagcgcaacg taaatcggaa tatcgccgat tatcgacaca ccgttgtcat tggcatactt
     2521 tttaagtttg ttccactgtt caaaaaattt aaactgcaca aactcccaaa acataacgtc
     2581 atcttcatgc gacgacttaa attcccataa cgaatgagaa tcacgttttc ttagtccgtc
     2641 ctcccattca agccaagact tgccgtcatt ttcattcttt atgctcataa acaacgcata
     2701 gtt
//
LOCUS       DAWACS010000173         2694 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_3979_length_2694_cov_2.330428, whole genome shotgun sequence.
ACCESSION   DAWACS010000173 DAWACS010000000
VERSION     DAWACS010000173.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3979_length_2694_cov_2.330428"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2418..2441
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          816 a          462 c          599 g          793 t
ORIGIN      
        1 aaagtcctgc aaggattatc gcagtcttaa cctgtgcgga cgcaacaggc attttgtagt
       61 ctataccgtg caattttgta cccgtaatat aaagcggaca atattcattt tcgatttttg
      121 cacccatcat tgaaagcggt tgtacgactc tgcccatagg acgtttttga atagacgcat
      181 cacctgtaat tgaggtatca aaattttgac ctgccaaaat accgcacaaa agacgtgtcg
      241 tagtaccgct gttgcctgta taaagcattt catcaggctt tttaagacct cttagaccgt
      301 tgccgtgaac ggttacgttt tcaccgttta tatctatatc aattcccatt tttctgaaac
      361 agtcaatagt tgaaagacag tccgcaccct ttagaaaacc gcttatttcg gttacaccgt
      421 ttgcaagcga gccaagcata acggcacggt gtgagattga tttatcgccg ggaactttaa
      481 tttgtccgat ggcttttttt attttctgaa ttaccatttc ttatcctccg tatattcccg
      541 ataacaaaac aaggaacatc ttaatttagt tataaaataa ttatatcata aataggatat
      601 aaagacaagt taaaatgcgt ggagggagat atgaaaaagc agagttttct taccggtgcg
      661 gtaatactta tgatagcaaa tgcaatatca aaaatactcg gtgcggtatt taagatacct
      721 cttacatata tacttaacga ggacggaatg gcggtgttta atatcgcatt tgaggttaac
      781 ataatgtttt tatcgtttat catttcaggt ttgccgtttg caatatcgaa aatggttgcg
      841 gaatcaaatt caagaggaga atacagtaaa actcacagaa tagtgaaaat ttcaacaata
      901 ttgcttgtgt gtataggcat tgtcggaagt gttgcgcttt atttcggctc accgtttttt
      961 gcacttgcaa tgaaagaaga aaaggcgata tatgcaatac aaatgattgc accgtcaata
     1021 tttttcgttg cactcggtac ggcatataaa agttattatc aaggtgtttc aaatatgata
     1081 ccgaccgcac tttcacaagt aacagaagcg attgttaaac ttgcggcagg atattattta
     1141 gctgtacttt ttttgaatta cggcgttgaa aaaacagccg gcggtgcaat aatgggtgta
     1201 acggcaggtg agattatcgc aacggcgata cttatgatta tgtattttta cggcagagat
     1261 aaaaagtatg taaagtccga aaagagcgat actgcggaaa tattaaaaga gcttatgtca
     1321 atcgcattgc cgcttttatg tgcgtcggtg gtggcgaatg caataaatgt ggcagataca
     1381 acgcttgtgc ggtcaagact gcttgacggc ggtttttcaa gtgatgaggc aagatttttg
     1441 tacggtgcgt acaccggtta cgcacttacc gtttttcatc tgcctgtcgg aatacttgca
     1501 acattgggtg taagcatact ccctgtaata gccggagcga tagccgtaaa caactcaaaa
     1561 aaagcacaaa ccgcgaccga tataggaata cgtcttgcgg ttatgctttc aatgccttgt
     1621 gcggttataa tgtacttgat gagtaatgaa atacttactg tattatttca taacagttca
     1681 tcgtccgcta tgcttacggc gatagcaccg tgcgttatta tgatgtgtgt gacgcagata
     1741 acgtccgcga tattacagtc ggcagggaaa ataatgctgc cgttttttac ggcactttgc
     1801 ggaagtgcgg taaagctttc aatgagttgg tatcttatag ctatgcccga aattaatata
     1861 tacggttctg cgataagctc aaatgcagca tatattcttg taatgatatt aaatttattt
     1921 gcaattcgta aatgtttgag tttaaaattg aatattacgg caataattat aaagcccgca
     1981 atagcgacgg tgcttatgtt cggtgttatg tacatttcat cgaactatat ttcggcgata
     2041 ttgggggacg gattcgtata tcttgccgta atgtgcggta tcggtatggg tgcatatgta
     2101 cttatgctgt ttctcacaaa cgcaatatcg gttaaagaag tgcggaaaat acttaaagga
     2161 tagacttgta aaaaataatg cggggtgata taatacaaga gattgaagaa aatatgaaag
     2221 ggatttgtta cttatgaaaa aatattctat tgatgattta cgcgatataa tggtacagct
     2281 aaggggcgaa aacggttgcc cgtgggataa ggtacagaca catcagagca taaagaaaag
     2341 tatgatagag gaatgttacg aggcgattga tgcgcttgac agcggtgatg accacgcatt
     2401 cgcaaatgaa ctcggtgnnn nnnnnnnnnn nnnnnnnnnn natgtgcttt tgcaggttgt
     2461 gttccacgca agaattgcgg aagaaagagg agcatttgac tttgatgatg tggtaaacga
     2521 aatctgtaca aagcttatta cacgtcatac tcacgttttc ggcaaggata atgcggtatc
     2581 ggcagaagaa gcacttggac agtgggagaa gaacaagaaa aaagagaaaa atcttgattc
     2641 atatacggcg atgcttgagg acgtaccgca ctatcttccg gcacttatga gaag
//
LOCUS       DAWACS010000174         2680 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4013_length_2680_cov_2.341333, whole genome shotgun sequence.
ACCESSION   DAWACS010000174 DAWACS010000000
VERSION     DAWACS010000174.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4013_length_2680_cov_2.341333"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          766 a          605 c          461 g          848 t
ORIGIN      
        1 ttaaaaattt ttgaaaaact tttcaaaaac ctcttgactt ctaatagtta gtcacctcaa
       61 tatacaaaac gagctgttaa tttctaacag ctcgttagtt tttattttat tacagctgta
      121 ctatctgtgt atcaaatgca ctaagtgtta cagtcttatc acctgcgtca gtcttaactg
      181 ttgcagtttg ttctgtatcc gcattattga taacaacaag ctgtttgcct tcaggataat
      241 agcagcactc tgtatttacg ttatctgtca aatacttttg gtctgtgctt tcgcctgcgg
      301 cagtaagaat aaggttaaga agtgttctgt tattaacttc gccatgctta aagcttgaca
      361 tataaacacc ggtaccttta ccgaacttat gagttgtaac tgtcggcata tttccgtcag
      421 ctgcaagcac ctttgtttcg ccgtcaacaa gataaacctt gtttttacct gcaagaaccg
      481 tgccgtctac aaccgcaccg ttgttttcaa cgtcaaacga atacttaccg tgacaaattc
      541 ttgcacctgt atcttcgctt acgccaagaa cgtgtgacat acggaagaat gtatcgtaac
      601 cgtccgttgc agacggttcg tttacaccga tgaatgcacc gccgttgtta acccactctg
      661 taagaagtga aacaaccttt tcgtctttcc attgttctcc gcctgaccat gcacttcctg
      721 caaatccggc attaataaca acgtcgtaat tgtcaagtcc cttattcttt aggtcgtcaa
      781 agctgatgaa ttcaacgtca aacggcatac ctgaaagagc ctcgataatg tttataagat
      841 caagttcagg gtgttcgtga tagtgacctg cacaagtcca tgaacgcaga ctgccccaga
      901 atgtaagaac ggcaactctc ggttttaaaa cgtatggctt acctgcctta tggaattcct
      961 ttatttctct gaattcgtct gcaatctttt cgatatagtc gttgaaatca gggaacggca
     1021 atgtcaatga aaggtaaccg ccaagaccga ttctgtcgat aggcgcacga agaagtgcac
     1081 gtcttacgtt cttccaatat ccctttgctt caagtgtagg attaccgccc tccataaatg
     1141 taggagcacc gccaagacct acagggaaca aatacgggtg aagtctgatt tcgtgagttt
     1201 caactgcgtc aacgcctgca caaagtcttg cctcaaaacc gttgaatacg cacttgataa
     1261 ggccgtcaaa gccgaagtcc ttaaatctct tgccccaagg ctcaacacct acccagctgt
     1321 catcatagaa tacataagct tttttaccgt atgtatgtac aaggtcaaca agctttttac
     1381 cgaagtcaac aacaaaatca ttgatgaatt ccatccaatc aagctttttc ttgtcacaag
     1441 tcatatgtgt agcgtgaagt tcacccttat tgataaaatc ctctgaagta agtgcataac
     1501 cgtacttctt ttcaaattcc tttagagcaa gcggagaaac cgtaaagtca tacgacgccc
     1561 aatctgagaa taaatctctg tttctctcgt ttgaacccca aatccatacg aagttataga
     1621 acattgatgt gaaacgaaca actgttgttg cagggtgtgt ttcgcaccag ttcttcatcc
     1681 aatcaagcat atattgctgt gtttcagggt atctcggatc aatctgcatt agatgttcct
     1741 tgtcccaatt atttgttgta tgattgtaca ttgaaatttc ttcccaaatt ctgtatgcaa
     1801 ggaagtttac tgtgtactta tgccaaggtg ttacgccatt taccgtaaca acgccgtttt
     1861 catatgtcca atccttaact tcttcatttg tagttctgtc gaatacctgc caatatgcct
     1921 ttgattcgtc agactcgtta atcttgaact gttcgttaaa gaagtccttc ataaggtcaa
     1981 tcttaacact gtcgctttcc gcaacaacag gatttgtcat aaggaatgac tgctgtaatt
     2041 tatctgtgtt ttgctttgcc cactcgttat ggtcacgaat aatacaaatt gtagaataaa
     2101 ttccgtaacc cgcatttata atttcaggtg acaattccgt accgtcagaa tctctgatta
     2161 cgtctgcacc ccatttttca gccatttcaa gtgttagctt ttcataaccg ctttcacccg
     2221 gaagtgtgaa accgccttta gtcttaccca tttcgttctc tcctttttgt tttttctcaa
     2281 tattagaatt atatcataac gaattgttat tgtaaagaaa aaagcggtaa ttctttataa
     2341 aaagaaaccg cttctttgtt aatcgtacaa attttcaagc aaattacaat aatccgaaaa
     2401 aacagtcgtg aaaatataat tcacaacaag cacctcatca ggtgaatact tatccaaaat
     2461 catctgaaaa tcgccgtcaa aaattctcgg atcgacaaga attacatttt tatagctttc
     2521 tataagccat ggtgcaagta cgtttgtata cgaatcttta agcacaacca acgttttacc
     2581 gtcaggattt ttctcattcg tcatttcgac aatcggatgg tcactgccga agaaaaagtt
     2641 ataattcgct ttacttctgt cgtacatcac accgttgtac
//
LOCUS       DAWACS010000175         2672 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4026_length_2672_cov_2.163164, whole genome shotgun sequence.
ACCESSION   DAWACS010000175 DAWACS010000000
VERSION     DAWACS010000175.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4026_length_2672_cov_2.163164"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          872 a          530 c          462 g          808 t
ORIGIN      
        1 ctcttatacc tgttgcgtac aaaagttcaa gcatagcctt gtcgcgtata cccttattat
       61 cacagttttt aggctgttcc aaaagaagtt caacttcttc gcctgataaa attttcggaa
      121 ttttcttttc cacatgaggt gcctcaagat tagaagtcgg atttgacttt acaacaccgt
      181 tttgcatcat aaacaaataa tacgaacgaa gtgatgcaag tgtccttgaa acagtcgagc
      241 ttgctctgcc ctccttttga agataaagca aatatgacaa taccgtggtc tttgttgtag
      301 atgatatatc cgttacgcct gttccgtcaa gataagaaat atattgtgta acatcacgct
      361 tatatgattc aactgtgttt agcgatttat ggcgtatatc tgtcataaaa cttgtgtatt
      421 ccttaacgta ggtattcatt ttgttcattc ctccgagttt actgtttctt tacgtatatt
      481 tcaaaaatat ttcatttata tttcaatgtg attataatta taccacattt ttttaatttg
      541 taaaccccta tttttcttgt ttttatgcca tttcagcgtt ttagcttaat ttcggcgaaa
      601 cccagctcat aaatgttgtg gtcaagtatc cctccgacaa tgacgcaaca caaaatatag
      661 aaattattat tatcataaaa aatatatacg aaaaaattat tttttttgat tttctttcgg
      721 aattaagtga atatttaatg cttacagccg aaaaaagcag taatgtcggt attgttataa
      781 gtattgtcgg cattgttgaa agcataacaa gcataccttt tgctccatag aatttaataa
      841 atgacgcggt tgtaaaacct attataaacc ctttccttac aataatcgct cccgttacaa
      901 taaaaccgaa tcgcaaaaat cccattaaaa atacaattcc gacaccgata agattagctt
      961 gtaacgaatt tttaaaaaca gtcatactgt tcttattttc gcttacagcc gtacaaaaac
     1021 tgcctatata attttttatc ccctctgcca tatcgccgct gtttttcgcc aaatacacac
     1081 tccctaccgt cacaccgaca acaagcaaaa aaataagcaa tcccaaaagt ccgttaccgt
     1141 gtttttcgtc ggctacaatg tcgtttaatt tcacacgtcc cacctccaat tcaatattat
     1201 tcaaaaaatc agtgttttat cactaattta ttgtgcttat cttcttatat tatttacacc
     1261 gccaattttt tttggtataa taatatagtt aaaaaacaca cgtaaggaga gagatacaat
     1321 gaacaaaaca ctgaaaaaag tcttaagcgg tgttatttca gcatctatgt tgtgttcggc
     1381 acttgcaata cctcacaccg cattggctga tgacgcactg cctacggacg gtctgcttat
     1441 ggacatcacg tttgatgaaa caggcacttc ctccggctca tttaatgcaa cggtcggcgg
     1501 taccgttacg gaacacggca gtgtaagcta tgttgacaac tatgacggtt caagcaaagc
     1561 attaagcatt tcaacagacg cagcaggcaa ttatcttgaa cttccgaaag gtcttttaaa
     1621 cggcaaagat gccgcaacat tttctttttg gattaaaccg tcaagcagat gggcgtttat
     1681 gacaacacct gtaagtgata agcaaaactt taattatgaa aagtatttag gtatgcttgc
     1741 ctcatcttca ggctataccg ttgaacgtta caacaacagc ggaacacgtc tgagcagcgt
     1801 taatgcaaac gcaagcggtg actggcaata cgtcacagca gtatttaccg ctgacggtac
     1861 aaaggtatat gtaaacggta aactattagc ctcagacact gcggcagttg acattaagtc
     1921 actgtttacc gccgacgctt ctacttggat tggtcacggt aattggtatg atgacaaaac
     1981 tggcaaattt gaaggttttt cgggtatgat tgatgatttc aaagtatacg gcagagcatt
     2041 gaacgaaacc gaaattaacc aacttgccgc aaaagctata caaagagagc aagaggaaac
     2101 tgtaaaagaa aagaattgtc ttgatattaa cactcaattc tatacaaatt acagtgttac
     2161 agagagtggc aataacgtta ccgtttcagt tccgtcccct tcagaggaca aatatgaaat
     2221 aattgcagct tcatactcaa acggtgttct tacaaatgca gcaaaaaaga gtaaatcaga
     2281 tgtaacatca gacaaaacag ttacttttga aaatataaag aaaaacgaaa atgacactgt
     2341 aaaagtgttc gtatggaact caatagacgg cttacaaccg ttaagtgaca acaaagcatt
     2401 ccaaatccgt acaggcgata aagtaaaagt gaaaacaagc gttacaaact acactccgtc
     2461 ggagcagtct gtttcattac aaattgttcc ttttgacaaa gacggaaagc agtccgatgc
     2521 tgtacagacg cttgattctg taacactcgg tattatggac agttacgatt ttacagatta
     2581 cataacaggt gacagtaatg tttcgtatta taaagttctt gtatcaagca cagttgatga
     2641 caagactgca acttacgaag caggttacat ac
//
LOCUS       DAWACS010000176         2660 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4041_length_2660_cov_2.554702, whole genome shotgun sequence.
ACCESSION   DAWACS010000176 DAWACS010000000
VERSION     DAWACS010000176.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4041_length_2660_cov_2.554702"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    260..269
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          903 a          478 c          591 g          678 t
ORIGIN      
        1 atacggaact tgttattttg cttggctgcg aacgtgagta caaggataat aagctggagg
       61 cttgggtgaa caggcacaat gaccacaagg aatataatgc ggcggtacgg aaagaatttg
      121 acggctgcaa aaatgtgacg ttgtttgacg tgaatgaata tataacgtcg gacgatgatt
      181 tcaacgacag tgtaaaccat tataaaaaac gtgtgtatta cttgatggca caaaaattta
      241 ccgaaatgat aaatgctcan nnnnnnnnna tgctcatgca aacgcagatg tagcaaagca
      301 aacgtcaaaa gcaaaacttg cgtatttaac attaaaacaa aaaattaaaa agattgttaa
      361 accaaacggt taaattttat attgacaaaa cagttgcatt agaataaaat taaactattg
      421 attaaaataa tttgggagat tataaatgag tatacgaaag gtaactatat gcttggcggt
      481 gattcttttc atatctgcca ccgtacttgc atttacatct aataaacgtg cagaaaaaaa
      541 ggctgaaaaa gaatatacgc ctaccgtaca gacggtcaca atgacggctg tcggcgactg
      601 tacgttggca actgacataa acgcagaccc aaacggcagc tttaaatcgg ttgccgagtc
      661 tttaaacggc gattattcat atttctttaa aaatgtttca ccgatatttt cggaggacga
      721 tttaactatc gtaaattttg agggtacttt gtcaaatcaa ggaacaagac aggataagca
      781 gtttgctttc aggggtaagc ctgaatatgt gcagatactt acatcgtcaa gcgttgaggc
      841 ggcaaacctt gccaataacc acagtgccga ttacggtgac gtaagtcttt cggacacgat
      901 aaaatatttg aatgaggccg gtatctccaa ttttataggt acgaataccg cgataagaga
      961 tgtaaacgga atatcggtcg gacttgtcgg aattgacgca cttgacgaaa cagaggcggc
     1021 aaaacttgaa aatgttatcg gttcggttaa aagtcttggt gcacagcttg ttatagtaag
     1081 tattcactgg ggcgaagaaa aggctaccgc tccgaacgat acacaaacag agcttgcaca
     1141 caaggcgatt gacgcaggtg cagaccttgt tttgggaaca caccctcacg ttttgcaggg
     1201 tattgaaaaa tacaacggta aatatatagc gtacagctta ggtaacttct gcttcggcgg
     1261 taacaataat ccggcggaca aggacacagt tatatacaga cagacattta cgtttgttga
     1321 cggggttatg caggaggacg ataatatgac gcttattccc gcaacaattt cgggtcatga
     1381 tgagtataac gactatcagc cggcaatagc tgagggtgac agaaagactc aaatagagaa
     1441 taagttgatt gaatattcga caccgctcgg actttctgcg cttaatttca gataatttgt
     1501 ttttactatt gacaaatggc agtaaagttt agtataatat aacacagatt agatgagatt
     1561 agaacagaca gagcatattt taatatgatt tttatgcctc tcggacttat ctgtctgaga
     1621 ggtttttctg ttttttcttg caaaaaagaa aggaagatgt aaaatgaaaa atttaaagaa
     1681 gattattgca atgggactaa cagccgcact atgtgccgtt tctttggcag gttgcggagg
     1741 cacacaaaac ggtggaactg acggagcaac agacacaaat gctaaagata catacaaaat
     1801 cggtgtactt caatacgcag accacccgtc gcttgataac tgccgtgagg gctttaagca
     1861 gggacttgat tcagagggta tcaagtatga tattacagaa tcatctgcaa aaggcgacga
     1921 ctcaacaaat acacaaatcg cacagcaatt tgtttcacaa ggtatggacc ttgtatgcgg
     1981 tatcgcaaca ccgtctgcac aggcttgcta caatgcggct tatgaggcaa aaattcctgt
     2041 aatttttaac gcagtttcag accctgttgc cgcaaagctt gcagaaagcg atacacaggc
     2101 aatggacgga attacaggtg taagcgatat gttgcctgtt gacgaacagt taaagcttat
     2161 ccgtgaaatg ttgcctgacg caaagaaaat cggtatactt tatacaacaa gtgaagcaaa
     2221 ttctgtttct acaatagaaa catataagca aaaagtaggc gaatacggtt ttgaacttgt
     2281 tgaaaagggt attacaaatg ccgctgaaat tccgcaggca gtagaccttt tggtaagcga
     2341 agttgactgt atctcaaata tgacagacaa cacagttgtt gaaaacttgc ctatacttct
     2401 tgaaaaggca actgctaaaa acattcctgt attcggcagt gaagaagaac aagtaggaaa
     2461 cggctgtata gcgtcagccg gtatcgacta cattgaactt ggtaaaaaag caggcgttat
     2521 ggctgcaaga gtgctaaagg gtgaggaaat taagaatatc ccttacgaaa ctcttacaga
     2581 aagtaaaatt actatcaaca ctgacgccgc agaaaaaatc ggagtaacaa taccgcagtc
     2641 aatcacagac agagctgagg
//
LOCUS       DAWACS010000177         2654 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4051_length_2654_cov_2.314352, whole genome shotgun sequence.
ACCESSION   DAWACS010000177 DAWACS010000000
VERSION     DAWACS010000177.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4051_length_2654_cov_2.314352"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          795 a          413 c          637 g          809 t
ORIGIN      
        1 gacatatcca tgggataatg ttgacgaagt tgacaaggac ggaagaatca gacaacgcta
       61 taagaatatg attaaattga gaaacagttc aaaggcattc tcaatcggcg attttgaatg
      121 tgttcataaa gtcggacacg tgtacggttt tgtacgttgt tatcatgatg aaaaatatct
      181 tattgttgcc aatctgggta caagagaaga aagagcacgt gtagatgttg cgagatacgg
      241 tatgagaaaa ctaaaatgtc aaacgagtga cgataacagc ttgcttgaat cgtctgacgg
      301 tattcatttt attgatatac cgagattatg gataaaggtt tacaaggtgg aaaaataatg
      361 caaggtaaat tttcggatta tttgcttgtt tcggatatgg acgcaacgtt gcttgatgac
      421 aatcacacca tatctgaaga aaacagacaa gcgatagatt atttcataaa aaacggcgga
      481 cggtttactg tcgcaacggg aagaatggtt gaggcagttc gtgcatatat gccgaatttg
      541 cacataaatg caccggcggt attacataac ggtgcgaaga tatatgacta tgaaaaagat
      601 tgtgcggtat ttgaacgatt tattgaggaa aacagaaaac aggcaataaa gcgtgtatat
      661 gatgattttc cgcaaatagg tttggaagtg tacagtgatg aaattgtgta cgtttacaga
      721 gaatgtgagg aaacaaagcg atttttgaca cgcagctatg aggttgttta tagtttaccc
      781 gatgaaattt ggcaaagacc ttggataaag gtgcttttga ttggtgaaag agaactgctt
      841 gacaagtacg agccgatata cagagccgaa tatgataacg gctatgcggt cagaagcggt
      901 gacaagtatc ttgacattgt tgcgaacgga gtttcaaagg gtaagggaat gcttgatatg
      961 gcgaaaaaaa tcggtattga gcagaacaaa attatagctg tcggtgacaa tatgaatgat
     1021 atttcaatgt tggaagtagc gggaatttcg tatgcagttg agaacgcaga agaaagcgtg
     1081 aaaaaaattg gggacaactt agcgccgtct aacaccggca gtgctatcgc atatatcata
     1141 aacagacttg aaaaaattgt aaaaacacag aattaaggct tgaaatatcg gcagttttat
     1201 gttaacataa ctctataaaa agtatttaag gaggtattta tgtttaacat atcactgttg
     1261 ctttcggcgg ctgttattat aatttgcgta cttgcaaata agttatcaaa caaaatcgga
     1321 atgcctgtac ttattgcgtt tatcgcactt ggtatggtgt tcggttcgga cggaatactt
     1381 aaaattcctt ttgaaaatta cagctttgca gaacagattt gttctgttgc gcttgtattt
     1441 attatgtttt acggaggttt cggcactaaa ttaagcgagg ctaaacctgt ggcagtaaag
     1501 gcactgttgt tatcgagcgt gggagttatt ttcacggcgg ctcttacaac agtgttttgt
     1561 ttttttgttt taaaaataaa attgctcgaa agtatgctta tcggtgcggt tatgagttct
     1621 actgatgcag cgtctgtgtt ttcggtactg cgttcaaaaa atcttaactt gaagtataac
     1681 actgcgtcaa tgcttgaaat ggaaagcgga agtaatgacc cgtttgcata tatgctgacc
     1741 gttatagttc tgtcgtttat gaagggtgaa aacagtgtcg gacagatggt ttacagtatc
     1801 gccgcacaaa ttgttttcgg tgttttgata ggttttctga ttgcactcgg tgcagtatgg
     1861 tttatgaaat atttcacttt taatacatct ggttttgatg cggcgtttgt gcttggagtg
     1921 gcggtgcttg cgtacgcatt gccgacattg gtcggcggaa acggttatct cagtgcgtac
     1981 atagtaggta tcgttttggg aaatcagaac atatataaca aaaaatcact tgtacatttt
     2041 tttgatggaa tgaccggact tatgcaaatg cttatatttt tccttttggg acttttgtcg
     2101 ttcccgtcaa aaatattaac tgttttgttg ccgtcaatat ttatagcact gtttatcaca
     2161 ttcgttgcaa gaccgttggc ggttgcgact gtgcttgcac cgtttaaaac aaaaatttca
     2221 caatatcttg ttgtgtcgtg ggcaggtctc agaggtgcgg cgtcaatcgt gttcgcaata
     2281 atggctatgg taggcggtgt gacaatggag caggacattt tccatattgc attttgcgtt
     2341 gtattgtttt caattacgct gcaagggtcg cttctcccgt ttgtcgcaag taaaacgaat
     2401 atgattgaca agaccggttc tgtactgaaa acgtttaacg actattcgga cgaaacggaa
     2461 attaatttta ttcgcttgaa tattgacagc ggtaacagat ggattgaccg tgcggtaaag
     2521 gatatttcac ttccgccgca aatgcttttt gcggcgattt acagaaatga cgaaacgatt
     2581 attccgaacg gtgaaacggt aatatatgag ggtgatacga taattctcgg tgcaccgtca
     2641 cacggaataa acgg
//
LOCUS       DAWACS010000178         2647 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4064_length_2647_cov_2.432485, whole genome shotgun sequence.
ACCESSION   DAWACS010000178 DAWACS010000000
VERSION     DAWACS010000178.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4064_length_2647_cov_2.432485"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          541 a          629 c          481 g          996 t
ORIGIN      
        1 atgtcgtact ctttctcaat ttgttcttta cgcttgtcca aatactcctc attcagcttg
       61 ttttttgcct ctacatattc tttgtggctg attataccct gtgcgtacat ttgttcggtg
      121 tacgtctgta ttctgccgat acctgctata taatcggcgg cactcatacc gttgtatttt
      181 tcttggtgtt ccaaccaatc acgactgtat tcggtcatat tgtcgtataa cgttgaacct
      241 atacttgaca tttctgtcgt atagtcctcc cacgtcatac gccctgcctc gacttccgcc
      301 atattgcggt cacgaatacg ggtaaatgcg tcgataggat tgtcaccgtt gtcgtcccag
      361 tcattcagtg ccgcgcgttc ttcaatgtac gactttgaca ggttgtttaa ttcctgcgtg
      421 cgtttctgtg tcagactgaa aatttgttcc tctatgtcgg caatatcctt gtcgttcgac
      481 ttgaatttct cttgaaattc taaccacttc tctaattctt gtgcggtcgt tactgcgtgc
      541 gttttggtgt aatgcgtcca atcgtccttg gctgatgtaa acgcatccga attgtctttt
      601 cctgttgcgt aatgcggtat acccataccg ttcattatcg ccttggtttg tgacgctgtg
      661 tacacctttg caccctttga caacggcaat actacgtcct tgccctgtgg tataaatgca
      721 cgtcctttgt caacgattaa ttctcgtggg tcagatatac ccttttcatc attaaccatt
      781 gccaaaccgc cctcaaaatt ttgtgtacct ttggcttttt tggctttttt tacgaacatt
      841 cccgaactgc caaacttggc cgccggaaca ttttgattac tcaatccacc aacttgaacc
      901 gtctgaacgg tcagtgttac tgttttatct tttacggtat ctaaattagc ctttgcactc
      961 tcaacgcctg ctgatgtgtt atcctgtgct gtgatttctg tatcgtgtgt tgtaggaatc
     1021 agatttattt tgcctgtggt taaatcaata acaccgatag actcgccgtt ttcggcaatt
     1081 agtttcgccg ttccggttgt accgtcatat tcggctattt tgtattctgt attatctatt
     1141 gttgcaatgg cgggtgttcc gtctgctgta aacatgacct cacactgttt gccgtcaagt
     1201 tgttttgtct ttttctcgac attatcaaca ccgtctgtgt tgccctctgt gtcaacagtt
     1261 acaacaaact ttttgccctc cagttgtcga actttggcgg tcaaatcatc aactacttca
     1321 aatccgtctg cggttatctt gatacgtttt tcattcggta tcagtcccat agcacgcgac
     1381 atttcggtca acttgtccgc tgtcatatca ataccttgct gacttccctc ggacatcata
     1441 tcctttaata tgccgtttat atcgcctttt tcaacggctt ggcgtacgct gtcaaatccg
     1501 tttttaatta aagcagttcc ctcaacgatt tcctcggtcg tcaatcccat tgtttgacct
     1561 gtttcattca ttttctgaac tacatcatca attttgccct tatttactgc atcctgcata
     1621 ttcgtacatt cggaatttaa cagactgaca ccgaccgccg tttccgcaga tgatgctccg
     1681 aactctgtca ttgaacgaac atagtcgtta attatgttgt tcagtgcggt accgtccccg
     1741 tttgccgcct gctcccacgc agcagacaaa ttctcaacac cgttcatagc caacgccgcc
     1801 gactgtgcat agctgttcat atctaattta ccgacgtcga taaattcttt catatccttt
     1861 aaggattgtt caattccggc gccgtcttga tttaatgcag atattttaat taattcagtt
     1921 tcatagtttg ccagttcttc tgatacgtcg cgtagttctt tatgagattg gtccagagcc
     1981 tgtacttggt cgtaatactt ttgggcttct gttgttgcta ccgaataatt tgccgcaata
     2041 gacgataaca cgccttgcgc attcttcatt gattggtctg ttgtgccgtt ttcgtatgcg
     2101 tgtccggaaa cttctttata aatttcttgt gcttttttgt agccctccgc cgcagttatt
     2161 tcattttttg aaatttttgc agttatgtca ctaacttttg atttagcctc tgaatacttc
     2221 gtctgtaatg ctaattcttt gttatagtta tcttccgcga tttggcggtc ctctttgtat
     2281 tttgcgtctt tatttattag atttgatagt tctgaacgtt gcttattgat attagactgc
     2341 aattcatttt tagacagttt ggttacttgt tcaacagcgt cgtccaaatt agaattatcg
     2401 gaattgatta caagattgta ttcctgcgat agcatttcct ttatttcttc taacttgctt
     2461 tttgcattgt caacttgttc ttgactgctt tcggggcttt caataaccat ttttaacgat
     2521 ttgatttgcc cctgtacttc attcagcgat ttgtattttt caaagctttc tttgaccttt
     2581 tcgttgccct tggatagtcc ctcgctccac ctgtattgcg attgatacca tttgtcatat
     2641 gcaactt
//
LOCUS       DAWACS010000179         2645 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4067_length_2645_cov_2.541313, whole genome shotgun sequence.
ACCESSION   DAWACS010000179 DAWACS010000000
VERSION     DAWACS010000179.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4067_length_2645_cov_2.541313"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    330..339
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          883 a          471 c          589 g          692 t
ORIGIN      
        1 gtataggacg cagtcatttt aagaagtccc ccattattaa gcgttggaaa actttttatg
       61 ggggtgttac tgattaatac agtattatta attgtaatga taatcgttgc aatcgtatta
      121 tgtgttatag gcgtcattgt tggtttcaac ttaggcgttg cacacagaaa gaaaattgca
      181 gaggctgaat tcggcagtgc cgaagaaaaa gccaagtcta ttgttaagga tgccgaaaag
      241 gctgccgatg ccaaaaagcg tgaactgttg cttgaggcta aagaagaaaa tcaaaaactt
      301 cgtgcagact ttgacgcaga ggttaaggan nnnnnnnnna aatgaactta atcgtcagga
      361 acgcagaatt aaccaaaaag aagaaaactt agacaagaaa acagataatt tagagaaaaa
      421 agaacagact ttaagtcaaa aactaaagtc acttgaatct aaagaaaaag aaatagcaga
      481 aatcaaagaa caacaaatgg ctcttattga aaagatttca ggtcttaccg ccgaagaggc
      541 taagactatc ttattaaaag atgttgaaaa tcaaacaaag catgaggcag ccatgatggt
      601 taaagaaatt gagcaagagg caaaggagaa cgcggaaaag aaagcaaaga acatagtttc
      661 tttggctatt caaaaagttg ccgccgatca cgttgccgaa accaccgttt ctgttgttaa
      721 tcttccaagt gatgaaatga aaggccgtat aatcggtcgt gaaggacgaa acatcagaac
      781 cattgaaact cttacaggcg ttgatttaat catcgacgat actcctgagg cagttattgt
      841 ttcaagtttc gatcctatca gacgtgaagt tgcacgtctt gcacttgaga agcttatcgt
      901 ggacggacgt attcatccgg cacgcattga ggaaacagtt gaaaaatctc gtaaagaggt
      961 tgaatcaatc atcaagcaag agggcgaaag tgctacattt gaaacaggtg tacacggtct
     1021 tcaccctgag cttatcaaat tgcttggtaa acttaaattc agaacaagtt acggtcagaa
     1081 cgtcctaaag catagcattg aggtttcgca tcttgcgggc gttatggcag gtgaactggg
     1141 cgtcgatgtc gcacttgcaa aacgtgcagg tttacttcac gacatcggta aagcagttga
     1201 ccatgaagtc gaaggctctc acgttactat cggcgcagat attgcaaaga agtatcgtga
     1261 aaacaaggat gttgtacacg ctattatggc tcaccatggt gatgtcgagg cgcagacaat
     1321 cgttgcctct ttagtacagg cagccgatgc aatatccgca gcacgtccgg gagcaagacg
     1381 tgaaaatctt gaaacctaca tcaagcgttt acaaaacctt gaagaaattg caaacagttt
     1441 cgacagcgtt gaaaagtcat ttgctattca agccggtcgt gaaatcagaa ttatggtcaa
     1501 acctgaaaag gtcaacgatg aaggtatgat tcttgtagca aaagaaattg ttaaacgtat
     1561 tgaaagcgag cttgaatatc cgggtcagat taaagttagt atgattcgtg aaacaagagc
     1621 ggttgattac gctaaataag cttgcattac tttgaaaaat ttggggttat tacagagcgt
     1681 aaatatttat gactttgata ctttgcgata aaggcactgt ataagccgtt tatgtttagc
     1741 ttctacccgt tctgtaatac tccttttttt atttattttg aacgaaaatt tattttaatt
     1801 ttagatatat tatacagttt tacacgtcct gtccgcccac gataagacag tgttataaat
     1861 aaatctgcgg gcagaaaggc tatggatttt attatgaaaa ttctatgtat cggagatatt
     1921 gtcagccgta taggacgcga tatgctcttt aaatatgttg acgatatgaa atatcagaaa
     1981 ggtattgact tagttatcgc aaacggtgaa aatgccaccc acggcagagg tcttgcccgc
     2041 aacgcatatg acgagcttat tcgtgcagga attgacgtta tcactatggg taatcacact
     2101 tgggacgcca aagaagttgt tgatattatg cgtaacgagg gaaacgtaat ccgtccggcg
     2161 aattacaata aaaagtgtcc gggtgaggga agtatcgttt ttaccgcaaa aaacggtgtt
     2221 aaagtcggag tcgtaaatct tatcggcaga acgtatcttg accccgccga ctcccctttc
     2281 gacgctgccg aacgcgaggt tgcaaaatta aagaaatcaa ccaatattat aatagtagat
     2341 tttcatgcag aggcaacaag cgaaaaactt gctatggcat atttccttga cggcaaagtt
     2401 tcagccgtat tcggaacgca cacacacgtt caaacagccg atgaagtcat tcttccgaaa
     2461 ggtacaggaa atataaccga cctcggaatg acaggtcctc aaatatcgat actcggcaga
     2521 gagaataatg caataataaa aaaatttatt acaggtatgc ctcaaaagtt tgaaattgcg
     2581 aacggaaaag gacaattttg cggttgtatt tttgacattg acgaacaaag cggtttgtgt
     2641 acgga
//
LOCUS       DAWACS010000180         2626 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4101_length_2626_cov_2.254376, whole genome shotgun sequence.
ACCESSION   DAWACS010000180 DAWACS010000000
VERSION     DAWACS010000180.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4101_length_2626_cov_2.254376"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    678..687
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          724 a          616 c          432 g          844 t
ORIGIN      
        1 tccttacggc gaataccgca tattttaatt tccatatctc aaattatcaa cagctttttt
       61 tatatccttt tcacgcataa acgcctcacc gataagtgca gcgtcaaact ttaatcctct
      121 cagatattca aagtcactgt gtttccttat cgcactttcc gcaaccttta ctttaccgtc
      181 gggtatcagc ggtaaaagtc tttcgcacgt cgttatatcc tcgtcaaacg tctgaagatt
      241 tctgttgttt acgcctatta tcctgccgta ttcaaccgct cttttgactt cttcttcgtt
      301 gtgcgtttca acaagcacat caagtccgat actgtctgcc aaagcgtgaa acttcttgta
      361 ttccgcgtcg gtaagtgccg ccattataag caaaattgcg tccgcaccgt acagtttagc
      421 ctcatatatc tgatactcgt caaccgtaaa atccttgcga agtacgggaa tgctgaattt
      481 acttttaata tccttaacaa attctatatt gcctttaaaa tattcaggct cggtaagtac
      541 cgacattgct tgaatattgc atttgtcata ttcttcggca atcttcatat ggtcaaaatc
      601 tccgcgtata agtccctttg acggcgatgc tttttttatt tccgcaataa ttgaaacatc
      661 gtcaatcgac acaagtcnnn nnnnnnngct taaattttct ttcgccttcg ctactctgat
      721 tttcgtagat gcaataattt tatcaagtat catatcaaat tccgcagcct ttcttcttaa
      781 ttaaatttct tagtttcagc cacaaattcg tttactttat ccataacatt accgctgtca
      841 atagtctgtt ttgcgatttc gacagcctct ttgtatgaat ctgctttacc gccgatatat
      901 atacctgccg cactgttaag aacgataata tcgcgtcttg aactcttttc gccgttaaaa
      961 atactttttg cgatttcggc attttcctta ccgtcaccgc cggtaatatc gcttgcagcc
     1021 gcatattcga taccgtaatc gtgcggatcg atttcataca cgacaatctt accgtcaata
     1081 atttcgttta ccaatgtttt acccgtaacc gtaatttcgt ccataccgtc actgccgcaa
     1141 acgatcatag cgcgtttaac gcccatttca ctcattacct tagcaaaaac gctgactttt
     1201 tcaggtgaga aaacacctat aacctgtctt gatgcatttg acggatttga aagcggtcca
     1261 agcatattga atatagttct gataccgatt tgttttctga taggtcctac atttttcatt
     1321 gccttatgga acttcatagc attcataaag cccatacctg tttcatttat acattgctca
     1381 acctgtttcg gctcaagcat aatgtttgca ccgagtgcct caagcaaatc ggacgcaccg
     1441 ctctttgaag atgcggctct gtttccgtgc tttgccacag tcacacctgc cgccgcacaa
     1501 acaaacattg atgttgtcga tatgttaaac gaattagcac cgtcaccgcc cgttccgaca
     1561 aagtctatgt actcatcagt atcaagcttg acgctgccgc cctttgactt catcatttcc
     1621 gtacaaccga caatctcgtc taccgtttcg cctttcatgc gaagtgccgt caaaaatgcc
     1681 gacaccaata caggtgacgc tgtaccctca agcatcatat ccattgcttt tgcagcttct
     1741 tcacgcgtaa gattttgtcc ttctgttact ttttttaata gttctttcat aattatagtc
     1801 ctcctctaat ctcaggcaaa atgtaaaaga aaagcccgtt tttgaccaaa acgagcgtaa
     1861 tatccctata gaatataaaa catcttttta attttgctca tctcgtctaa tctaaattta
     1921 ttatagcata gcataacaaa aaatgcaata ccggaaaatc gaattttcta cattattttt
     1981 ttcaaatccc ccttgtaata tttgtttaaa tattgtataa taaatgtatc aatgaaacag
     2041 gggtgaaaaa cttaatgaaa agtttcgata taaaaacaaa aatttatttc ggtgacaatg
     2101 cacttgacag acttgcagac cttccttata agaaagcgct tatcatcaca gacccgtttg
     2161 ttgtaaagag cggtatgcta cagatgatta catatcgttt aaacgacggt tatactgaat
     2221 ttgacgtatt ttcagacgtt gttccggacc caccgattga aaagatttct cttggtgtta
     2281 aggcaatgct tgactatgat cctgacgttg ttatcgcaat cggcggcggc agtgccattg
     2341 acagcgcaaa atcaattcgt gaatttgcaa caagagcatc tcaaaaagaa gttgcactta
     2401 tcgctattcc tacaacttcc ggtacaggca gtgaagttac aagttttgcc gttgtatctg
     2461 atccggcaaa tgatataaaa tatccgcttg tatcagacag tatgttgccg acagaggcta
     2521 ttttggacgc cgaccttgta aagagcgttc cggcaaacat aacagccgac acaggtatgg
     2581 atgtacttac tcccgcaatc gaagcatatg taagtatcaa caacaa
//
LOCUS       DAWACS010000181         2619 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4111_length_2619_cov_1.932137, whole genome shotgun sequence.
ACCESSION   DAWACS010000181 DAWACS010000000
VERSION     DAWACS010000181.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4111_length_2619_cov_1.932137"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1921..1930
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          883 a          454 c          605 g          667 t
ORIGIN      
        1 gctttaactt gtatgaagac gatattctta cacctattga cgataagagt cagattgaat
       61 ttacatcttc agatacttca atcggcaact ttgcaagcgg aattgatgaa cttcatacaa
      121 catcaccgac aaaggcagga cctacaatcg taactattga aaataaggct aacggatata
      181 aagcattcgt aactgtaact gttaagggtg acacagtatg gccttcaggt tcaaaagtag
      241 cagtaggcaa gaacttctct attgctttaa aagaaaacgg aactttgtgg acatggggtg
      301 acaattctca cggtcaactt ggtgacggta cattattgag caagaaatat cctgtacaag
      361 tccttgatga agacggaaat ccgattaaag atatagtaag attggcagca ggtgacgact
      421 atgtatttgc cgtagataaa aacggtaaga tatatacata gggtgtatca atttccggcg
      481 gatcagatat gacgtcaccg gtagaagtag aaaatacaat cggcgatgaa atcgaagata
      541 tagtatcagg ttcaaattat gtacttgtac ttacaactca cggcgatgta ttctcttacg
      601 gtgttgataa ctcacaaggt caacttggca atgatgttat agctccttct actgcatcat
      661 ttactaagat tagtgcgctt aaagatatta gatcaatagc cgcaggcgat agccataatc
      721 ttgcagtgga tacaaacgga aaagtatgga catggggtgc gaacttgcat aaacagcttg
      781 gtagaacggt atcttccggt ggcggttcgg gaagtagcgg cggaacatcg gacgatgatt
      841 caagtgatgc cgatgccgac ggcgacgacg cagatactgc actacgagca acaactgcaa
      901 gtgcagatta tgacgcaact ccggcagttg tagaaggttt gtcaaatgta aaatatgttg
      961 ctgcaggcag tgcacacagt gtagctgtaa cagaagaaac acttgcagac ggaacaactg
     1021 ttacaaaagt atatgcttgg ggttacaact caaagggtca acttggtata gatgataaga
     1081 ctgtaacaga atcagtaaca cctatgcttg ttgttgccgg cgaagcggca ggtaaggatt
     1141 cgactgttga tgcaaacagc gatgaatact tatcaaatat tcttggagta acagcaggtg
     1201 gaaaccaaac actggttata acaaataatt atgaagtata tacatggggc ggagatgaaa
     1261 agggtcaact tggcgttgat tcaaagatag acagatttgc tcctgtaaag gtatggaagg
     1321 gagatacatt caatcaagac gaagatccat atctaaagaa cgtaatcggt gcggcaatgg
     1381 gtgcaaacca taccgtaata atcagagatg acggttatgt atgggcatgg ggtgataata
     1441 cttcaggtca acttggtaca gtaactgtcg atttggcaaa gacaccggta caagtaggta
     1501 atacaaattc aaatacattg tcattccaaa aggcaatagt atatactgat gaaaattcaa
     1561 caggtgatac aatatcacca ttaccgtcaa gcgttgtaat agacaagact ggtaaaattg
     1621 aaattgatat gtctgaaata tatgaacagt atatggttgg tttcaatttg ttcaataaag
     1681 ctgataagca aaagattgcg tcagaagata ttgcaaacat tcaatacagc tcgtcaattc
     1741 caaatatcgc tacagtttct gcaaacggaa atactgcaac tgtaatgccg acaggtaaca
     1801 cgttcggtac aacagtaatt acatttagaa acagcagaaa cggatatgtc ggaacaatta
     1861 atgttactgt taagggcgat aagatggaaa gcatcgttgc atcatcgctt acagcaggta
     1921 nnnnnnnnnn acggtactaa gataacaaga ggctatccaa ttcaggttaa attccaaaac
     1981 ggaagagatg acagtgcaag tacatttggt ggcaataatg cagctactgc tattgcggca
     2041 ggaaataatc acttgctaat acttgctaac ggcgtcgtat atgcagtcgg cgataatagt
     2101 aagggtcaac ttggtaacgg ttcgacagga actactgaaa gtatagttgt acctgtaatg
     2161 attgacgaaa caactacact tacaggcgta aagaacattg ccgcaggtga tgatttctca
     2221 atggcactac ttgaagacgg tagtatatat gcttggggta gcaataaatc aggacaactt
     2281 ggtgacggta ctacaactga cagtgcatat gcagttaagg ttttagccgg cagaagtggt
     2341 acaaacgcat atgatcctga taataatgat tattacttga atgacatagt ttcaatttca
     2401 gccggtcaag catttgcaat ggcattgaag aatgacggaa cagtttatac atggggtgac
     2461 aatagcaagg gacaacttgg tgacggtact cgtgtaaata aaccgactcc aattcaagtt
     2521 cttcgtggcg aaagtccgga agaagaaaca aatgtagctc cggcaggcag tgattactta
     2581 attaatatag taatgatttc agccggtgca aatcatgct
//
LOCUS       DAWACS010000182         2594 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4162_length_2594_cov_1.840882, whole genome shotgun sequence.
ACCESSION   DAWACS010000182 DAWACS010000000
VERSION     DAWACS010000182.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4162_length_2594_cov_1.840882"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    592..601
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2130..2139
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          809 a          451 c          577 g          737 t
ORIGIN      
        1 gctttgatac attcgtttca caaggcggta acaatctttc tggcggtcaa aaacagcgta
       61 ttacaatcgc acgtgcgtta atcaaaaatt cgcctatact tattttggac gacagcgcca
      121 gcgcacttga ctatgcgaca gacgcaaatt taaaacgtgc tattaaagaa aataccgaca
      181 gtcagacggt tataatcgtg tcacagcgtg taaattcggt gaaagacgcg gacagaattg
      241 cggttatgga cgacggtgaa attgtcggaa taggtacaca caaagaactt gttgatactt
      301 gcgatattta cagggaaatt tgttactcac aggaacagct tgactaagaa aggggtaaaa
      361 caatgaaaaa caataaaaca ctttcaaagc ttaactctta tattgcgggt aacagaaaat
      421 atcttgtttt ggttataata tcggcattgc ttgcaaacat atttatgctt gttgcaccgt
      481 atatttcggg tagagctatc gactttatca aaggcgaaaa taacgttgat tttcctatgg
      541 ttgcgaaatt tataggaata ctttttgcgg tttatgtgct taatgctttg tnnnnnnnnn
      601 nttacaaacg cactttcaaa ccattcgatt gaaaaaatgc gtaaagacgc attcggcaag
      661 atttcaaaat tgccgcttaa attttttgac ggtcattcac acggagatat tatcagccgt
      721 cttacaaacg acatagacgc agtttccgaa ggccttttgc agggaattac acagctgttt
      781 tcgggtatcg taacggttgt cggttcgctt gtcttgatgt tcttgcttga ctggagaata
      841 acactttgcg ttattgttat tacgattatt tgtatattcg tttcaaaagc gattgcgact
      901 aattcgggta aaatgttcag attacaggca cagactatcg gtgaacttaa cggttatgtt
      961 tcggaaacgg tcggcaattt aaaggttgtc aaggcattcg gttatgagga caagagcagt
     1021 gaagtattcg gcgagataaa ctctcgtctt tacgactgcg gtcaaaaggc tcaattctac
     1081 tcatcgcttg tcaatccgac tacaagatat atcaataacc ttgcgtatat ttctgtcggt
     1141 gttcttggcg gacttgccgc acttgcggga catttgtcgg taggtataat atcaagtttc
     1201 ttgatttatg ctacacagtt tgcacgtccg attaatgata tgacaagtat tttaactcaa
     1261 cttcaatctg cacaggcagc ggcggcaaga attttcgcac ttggcgaaat tgagcctgaa
     1321 acacctgacg aggacagacc cgaacttgaa gttaaaaacg gtgaagttat gtttaaagac
     1381 gttgatttct catataataa ggataaagaa cttatcaaag acttgaatat tgctgcaaaa
     1441 ccgggtcaaa gagtagcaat agtcggacct acgggtgcag gtaaaacaac tatcgtaaat
     1501 cttttgatga acttctacgg tgtggataag ggtactatat tcgttgacgg acaggctatt
     1561 gattctgttc agcgtgacag cttgcgtaag aatttcggta tggtacttca agatacttgg
     1621 ttgtttgcgg gtactgtaaa agaaaatatc gcatacggta aagagggtgc gacagacgaa
     1681 gaaattataa atgccgcaaa ggctgcgtct gcacacggct ttatcaagcg tttgccgaac
     1741 ggctatgata caatgattac agaggacggc ggaaatttgt cgagcggtca aaaacagctt
     1801 ttgactattg cacgtgcgat gttatcagat cctaaaatac ttattcttga cgaggcaact
     1861 tcaaatgtcg atacaatgac agagcagaga atacaaaagg cattcctaaa gatgatggaa
     1921 ggcagaacaa gctttattat cgctcaccgt ctttcaacaa ttcgtgaggc tgatttgatt
     1981 cttgttatgg acaagggcag aattattgaa cagggtactc acaatgaatt gcttgccaag
     2041 aacggatttt atacaaaact atataacagc taatttattt ggtgtgcgtg aaaatgcaca
     2101 ccaaattttg cattgggtag gagggtaaan nnnnnnnnng acttgtctta gccggcggcg
     2161 gcgtcagagg ggcatatcag attggtgtat ggaaagcact taaagaactg aaaattaaag
     2221 tttcggcggt gtcgggtgtt tcaatcggtg cggtaaacgg tgcgcttttc gtgcaaggca
     2281 gtaaaacaaa agccgaaaga ttatggaaca aaattgcgat tgacgatatt atattgttgc
     2341 cgaaagaaat ggagaatgac gaaaatcttt ttaaagtcaa aaacttaatg aaaatcgcaa
     2401 aagaaattta cagtaacaac ggacttgata tgtcaccgct tgaaaatctg ttaaatgaaa
     2461 tagtcgacga ggataaaatt cgtaattccg aaattgattt cggtatagcg actttttcac
     2521 tttcggagaa aagcgaaaat tattatttta tcaaagacat accttacggt aaactgaccg
     2581 aataccttat ggcg
//
LOCUS       DAWACS010000183         2588 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4177_length_2588_cov_1.842874, whole genome shotgun sequence.
ACCESSION   DAWACS010000183 DAWACS010000000
VERSION     DAWACS010000183.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4177_length_2588_cov_1.842874"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    493..502
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2322..2331
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          788 a          473 c          458 g          849 t
ORIGIN      
        1 atcagcgaca gcgacaacag cgtttgccgc cccgattcca agagaaagcg cgccgcttaa
       61 tgcgacagaa gcacaaatcc aaatcactga aaatattgta ggtgatattc ttgatgaagt
      121 gcagacgggc aagctcggtt acacattagc tgccggatat gctaatacaa aaatccgcaa
      181 ggctgtaatg gcaaatcaga cagacggtaa cggttacggt atcttatcgc caatcgcgca
      241 gaacgcaatc cgcgttatga gggataaaca gttaagacct gacgcatacg caaaagcgga
      301 ggaagaatta aaggtattga ttgcagatgt gattacagcg gttcagaacg gtatggatat
      361 tgacactgca acaaaaacgg cttatgagaa aatctatcaa tcagcagaca cgtcatttaa
      421 ttatgacgag caattttcac ttgatacctg ttatcgagat attcccgctg tcggaatgga
      481 aatgtttaca gtnnnnnnnn nnggaaattg ttattggagg ctgccgtaaa atgaaaattg
      541 aagaaataga taattgtgat gatttagacg atataaaggt tttcgcaata ttggttactg
      601 atgttccaag caaatatgta gcccaagcaa agaaaataga cggaaagtat tataaagagg
      661 attgttttgg aatagagatt tcatatcacg ctgacgagga caaatatgtt atatcgtccg
      721 aatatgataa acaactgtat tatgtggatt ttaacggtaa ttggcattgg ttggattata
      781 cttttacaca agcagaaaaa gacgcagcaa tagagttatg caaaaaggat ttacaaaagg
      841 aagcataacc aaagcggtta tgcttccttt ttctctttga ctatataata ttcttttcta
      901 acaactcggt aacttctctt acgctacgtc cttgtgaatg tatggcattg agcaaatttt
      961 ggatttcttc gtttgttggc ttttgtacac tattcttcgt tttaggagta tggtcattta
     1021 tatatttttg aataagtaat actaattcgt taatcccaat ttcacccaaa ttctttattc
     1081 cccatatttg cggcaaagtc aaatttagta gtttttctaa atcaataata tttttaaatc
     1141 gtataatagc agacacgact cttgcagaca aacaaagttg tgagagcgga atgtttttgt
     1201 agatttcgga ttttacatta aaaatttctg ctaatgtata atcagatgtt ttatatttat
     1261 tcgttaattc ttttatgttt tgtttatctt gttctgataa tttttgatat agggtagatt
     1321 ttaaatccat atgtaacaac tcttttcatt aaataaaatt gcataataaa agtgcgtcgc
     1381 ttcaatagag gcaacgcaca aaaaatgcga tactctattg tctgcgaaaa caatttttat
     1441 actttccaat atacgttgta acatatggca caaagtatta aaaagagtac gcatttttat
     1501 catatgataa aaatgttttt cttcgtgcca aaaaaaattg tatgcaaaaa aagctataaa
     1561 tataaaaatt gttttcgcat ttataaatat atcataaatt actattattg taaatacctt
     1621 tttacatcaa aaaaataaga caggaataac ggctaatata acagttattc ctatctatat
     1681 ttaatataaa atgtccgcgt tttcaatcct attctaaggg tatttgatat gctatcatta
     1741 ctttatcaat tttaggcttt gaaacacttg attttatgcg gttttattcc cttaaaatgc
     1801 ggacatagac cgcttatttc ttttccgttc agcgtcttta cgcaatcgta tctttcggcg
     1861 gcatttatca caatattttg aattgcgccc tgtcggcgta tattcattac cgcaaaccgt
     1921 acattttttc ctgttcttcg gcttcatcag ctttatgtac aattccttat cattcggcaa
     1981 aaccgctgtt ctgaaccatt tacaattaag tgaatatgaa atactttgag ggcagacgca
     2041 aggctcgccg tcatcaagta aaatacaatt cccgttgtca taattacagc attgattttt
     2101 tatcaatgct cggctccgcc gcgcctgttc gggcgtcatt cgtataatat cactcatagc
     2161 gtttcctcac gcggcttttt ctgcggctgc ggcttatcag tattgttttg ttccttttgc
     2221 aatctgtcta actttgcttt aattgagggc ttgacctcga ccgccgttat ttttccgtta
     2281 attctcataa acatttcagg tttagcaaaa cgctctgaaa annnnnnnnn natattatcc
     2341 tctgcgtcat aaagcgcacg gtttaattcc attctctcat ttttgccctc gtcattacaa
     2401 ataagaccga caggctccgc aaacggatat acaacttcaa ttctgccgcc aacctctttc
     2461 tggagggctt caagacctgt cgaaatttcc tttatataag gctcctgcat aggcggcaca
     2521 attagaacgg tcattttttg cggctccgta ggtacggtaa cgcgatattc gtcaggtatg
     2581 tttttacc
//
LOCUS       DAWACS010000184         2573 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4207_length_2573_cov_1.656076, whole genome shotgun sequence.
ACCESSION   DAWACS010000184 DAWACS010000000
VERSION     DAWACS010000184.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4207_length_2573_cov_1.656076"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    694..703
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1000..1009
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          720 a          503 c          399 g          931 t
ORIGIN      
        1 acataaatat tcctccataa aatacagaaa aagctgatgt ttctgcatta aaaaatagtg
       61 cagaagtcat cagcttaaat aagcggtttt agtattatac taagggagaa cctcattccc
      121 tttatttaat tcttgaatat tatatcagat ttatttgtga aaatcaagtc ctaacttaaa
      181 tatttactat aattgttcca atatcaacta atttaccgtc tttaagcgga ataatatcaa
      241 cattatattt atggcaaaac accttaattt gttcactgtc ggggtgagaa ttaatgtcac
      301 cgtttacagc aagcgtgcta ttatctataa gtcccgttgg tccaccaata aagccgtaat
      361 caagttttcg caattcaata tgacctgatg atatttttag gacatctatt cccttttgtg
      421 ccactgtctt ggctattccg ttatcagacg ttataattgc attattgtct acaatacaaa
      481 tattgcattt actgtacccc tgcttgacgt taataatttc tttattgtga ctatcaagaa
      541 tcgttttatc agtatatgct ttattacaaa taacataatc ttttaaagcc gtagcattgt
      601 accacacgtc atacggatat tttgagtcta tattgttttg tcctttaatt aaagtacact
      661 ttttaggcat atattttttg taatgttcat atgnnnnnnn nnntgaattt gaatatctgc
      721 gtgggttgca acggagtcat acagattatc aatctttaaa gtcggaatta ctttgatatt
      781 taatttttca agagtatata tagttttatc atctgctctg caatctataa ctgctatttt
      841 tttcaatttt aaaacctctt aaaatcaatt tagtttacat aatattttta tggtaaacta
      901 attattataa gtatctaaat aatcaactgc ggtttgtgac aaaatcgcac ttccaatagg
      961 taaagcgtct tcgtctgcta taaacttcgg actatgtatn nnnnnnnnnc gttttctcca
     1021 aaatatgaaa aatcatcccc cgccattgcc ggtttatcaa gtgtcactac ttcgtttatg
     1081 caggtaagct tttttgcact gtttctgatt acatcattca tttcactgtt attaacaaca
     1141 ggcggaaaaa gtttattgaa tgtaaaatca atagttgttc catgcttttt tgctgtttca
     1201 tgtgccgttt tatcaagtaa ttctatgagc atttcacgag tttcattgtc aagcgaacgt
     1261 gccgtaccca taatttcagc agtttgcgga atggcattat tgcaagtccc gccgttaaaa
     1321 tagcaaaccg tcataacaca cggcttgtct tttagaaccg ttgcatttat tttttcaaat
     1381 tccgttataa ttgccgtgcc gatagtaact ggatttacgc atttttcagg tgcggaggcg
     1441 tgaccgccga caccgtgaac aacaatttta aaatcgtcgg gcgacgccat aattgagccg
     1501 tccttaactt ggatattgcc cgtcttttct aacggttcaa cgtgaagtgc aaaagccgcg
     1561 tcaacgtgcg gattttcaag tattccgtcc tcaatcatag gtaatgcacc gcctactccc
     1621 tcttcagcag gctggaaaac aagttttacg tttccgttca actcatcttt aatatcgttt
     1681 aaaattttag ccgcacctaa aagacacgtt gcatgaatat catgtccgca tgcgtgcata
     1741 tatccttcgc gttccgattt tatttcaaat ttgctttcgt cttttatagg taatccgtct
     1801 atatcagcac gcaaaagtac agttttatta ctgtttttac ctttaatcaa ggcaactgtt
     1861 cctgttttag ctgaggttgt ataaggtatt ccgtaatcgt caagtttact gcgaataaat
     1921 gaagatgttt ttatttcttc aaaatcagtt tcaggagtac ggtggaggtg acgtcgtatg
     1981 gaaatcagct catcctttat ttgaaatgct ttttcttcaa tcattttatc atctcctcaa
     2041 attcatcttc actgataacc gttacaccca agctgttcgc cttgtcaagt ttacttcctg
     2101 cgtcttcacc ggcaagaaca tatgttgttt tctttgatac acttgacgat gttttaccgc
     2161 cgtaattttc tattattttt cctgcctcac ttcgcttata tttagaaagt gttcctgtca
     2221 gaacaaatgt cataccctca aaacgtctgt cgataataga atcttcactg tcgtctatac
     2281 agtttacacc cgctttttca agcttatcaa taaaagcgat tgactgctct tgcataaaaa
     2341 attcaacaac ccattgtgcc attttaccgc ctatatcggc aatttcggta agttcttcaa
     2401 cagacgcatt tctcaaatta tcaagagttt tatattttgc cgataaaatc tttgcggctt
     2461 tttcacctat atgacgaata ccgaaagcat ttatcagtct gtataatggg tttgtttttg
     2521 acttttcaag agcgtttata agattttgtg ctgatttttc acccatttta tcc
//
LOCUS       DAWACS010000185         2555 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4238_length_2555_cov_2.277600, whole genome shotgun sequence.
ACCESSION   DAWACS010000185 DAWACS010000000
VERSION     DAWACS010000185.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4238_length_2555_cov_2.277600"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    544..553
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1460..1469
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          851 a          442 c          587 g          655 t
ORIGIN      
        1 ccataacgtt gcacttatta aagaacaaaa cgaaaatcat acacagtatg atgttcctga
       61 agataaggcg ttcacttcaa agaacggtta tgtattcgca tggggcgata attcagaagg
      121 tcaattcggt aagacaacag ctaattcaaa cagctatgca cctgtaagag ttgatattcc
      181 tgatgatgaa gtaattaatc atatttcagc tgaaaagaat tcaaaccttg caacaactgc
      241 cgataataca gtttgggcat ggggcaacca tgcagatggt caaatgggag caaacggcac
      301 aagctcggct cctgtacaaa tcgtatcaaa tgacggcgga gtatggtcat catcgaatgg
      361 cattaagggt gccctaaacg gtgctgccgg cggagattca atgtctgcat atatttcaga
      421 cggtacagtt tggacatggg gttcaaatag ttcaggtcag cttggcgatc agacaaatgt
      481 taataagaac taccctgttc aggcaattac agaaccggca acaaagatga ttaaggttgc
      541 acannnnnnn nnncgcgtat gtttataaga agtcggattt gacaactcct gttaaggaac
      601 ttgaatatcc gaacgaaatt atgatagatg ttgacgaaac tgtggttatt gacagaattg
      661 ttgtagaaga agttcttgca tttaacctat acttcgataa gcgtgtgaca gaaatatcag
      721 tagataatat tgatgctaag tcaaccgatg aagaggcaat agaagttaca tcaactaatt
      781 caaccgttaa ggcaaaatca ccagaaaatg cagtagctgg tgacagtgct tatgtaacaa
      841 tgtcagtgcc atatgacggt gatactgcaa caggctttat aagagtaggt ctaatcgcag
      901 acggaacaac atcacttccg gaggcgtcat taagtacaac aagttctgca atcgtaaatg
      961 cagacggaac agtaagcgca tggggcgata acagctttga taagttaaac gtatcattgt
     1021 ttgataatca atctgatgta tatgctcctg gtgttgtaat gaaagacaat gacggacaag
     1081 ttatatttaa taacgtaaaa gaatttgcgt caggtgaaaa cttcagtgta attctaacaa
     1141 atgacggtga agtatacgct tggggtacaa atgaaaactc tcaaacaggt ataggcaatg
     1201 caggctcaac tgcttacgca tatccgcaaa gagttatgaa cagttcagat acaccgctat
     1261 cagatattat atctgttaag gcatattcga ctcatgcagt tgcactttca aaagacggta
     1321 gagtatatgt atggggtgca gtatctgata agattggtca gaacagatca agacagaatt
     1381 attatgcaaa accattgaga attactaatg tacaagctat cgcagctggt gaaaatcata
     1441 cagtagcatt gaaggcagan nnnnnnnnng ttcaaatgat tcaggacaac ttggatctga
     1501 tacatcatca actccgctac ctgtaatggc agagggtgta agcagtgtgg ttgcaatcgc
     1561 agccggtgca gatacatcta ttgctcttac agcagacggc agtgtatata catggggcgg
     1621 cggcgaaagc aacgcaacaa aggttcttga caatgctact cacgtagacg ctaagggtga
     1681 acaattcacc gcagtatcgg ttgacggcag tgcatacgca tggaacaaga acgaaactcc
     1741 tacaaaggtt gaaggactaa gcaatgttat tacatctgcg gctggcggta atcacgcaat
     1801 ggcagtcctt tcagacggta atgtatatac atggggtgaa aacagtaatt atcaacttgg
     1861 tgacggcaca aataatacat caacatcacc gattaaggta aataaagcgc ctgaaggcat
     1921 tcgtgtaact aaggcggtag taggtacagt ttcatacaca gacaacgata aacttttata
     1981 tgagtttaat cttgaaagtg gtgatgaact tggtattgac ggagttaaga agttcgttcc
     2041 ttatcgtttc aatgtacttg gtacaaagag atgggaaaat gttatgaatt acagacttga
     2101 agctcttgat tcaaatgtag cagtagtaaa tggaaaaaca atttcgacag tatcaggtcg
     2161 ttacggaaca acttacataa agattattga acttaacgat gacggaactg acggtgaaaa
     2221 cgttggattt ataaaggtaa atgtaatacc taacggtgca ttcacagcac ctatggttgc
     2281 agccggtaca gattttgtta ttactctaaa ggatgacgga actgtatggt catggggttc
     2341 aaacttacat aataaagtaa gtgcgaaatc tgatgaatat gtagatgttg caacacaagt
     2401 agagttaagt gaaaacactg ctcttacaaa cgttgtgaag attgccgccg gtgaaagtca
     2461 cgctcttgca cttactgcag acggtaaggt ttacgcatgg ggcggcggtg aaaaaggtca
     2521 gcttggtaat ggttataatg aagatagcaa ttatc
//
LOCUS       DAWACS010000186         2548 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4251_length_2548_cov_1.653028, whole genome shotgun sequence.
ACCESSION   DAWACS010000186 DAWACS010000000
VERSION     DAWACS010000186.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4251_length_2548_cov_1.653028"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          744 a          554 c          422 g          828 t
ORIGIN      
        1 ttcttcaact gtgatgacgc cgtctgctgt taccttttcc attgcctctg caattagctg
       61 accgatttct tcattagcag ctgaaaccga cgcaactctt gcaatatctt ccttaccatg
      121 aactttcttt gctgtagaaa gtagcttttc aacagccgcg tcaacagcct tttggatacc
      181 ctttcttacg atcatagggt tagcgcctgc tgcaacattc tttagtcctt cacgaacaag
      241 tgcctgtgca agaagtgtag ctgttgttgt accgtcaccg gcaacgtcat ttgtctttgt
      301 tgcaacttct tttacaagct gagcacccat attttcaaat ggatcctcaa gttcaatttc
      361 cttagcgatt gtaacaccgt catttgtgat tagcggtgca ccgaatttct tatcaagaac
      421 aacatttctg cccttcgggc caagtgtgat tttaactgta tttgcaagtt ggttgatacc
      481 gctttcaaga gcgtgtcttg cgtcctgtcc gtatttaatt tgtttagcca taataataac
      541 ctcctaatat attagtctac tattgcaagt atgtcgcttt gacgaagaat tgtatattcc
      601 tcaccgtcaa ccttgacttc tgtacctgca tattttgaag taagtacctt atcaccaacg
      661 gaaacttcca tcttaacttc ctttccgtct acaacaccgc cgggacctac tgcaacgatt
      721 tcagccacct gtggcttttc ctgtgccttt gaagtaagga taattccgct ctttgtcgtt
      781 tcctctgctt caagcatttt tataacaact ctgtctgcca atggtttgat gttcattata
      841 tattacctcc taaaatttta atcaagttca ttgttagcac tcacacttct tgagtgctaa
      901 caatgctatt atgttctttt ttgctctatt tagcaaagtc cgtttttttg atatattgct
      961 atatttactc ttattttctt tgtttttctt tttattacgt tcaatatctc ccttttggtg
     1021 ttttaacaaa ttgttcataa acttaatact gctttaactt acgaagtttt caaacatctg
     1081 ttgtgcctca tcaagattat caaattcaac accttttttg agttcatcaa tatcctcgct
     1141 cggaaataca ttttcgctta tttcctcact caaaattgct gttttattat cccctacaca
     1201 tttattaatt tgaagtaaac cgttctcaac cttaacttca tatactgtag tttttacatc
     1261 atttgcattc acctcttcaa ccgccgcaac cgtaggcata ggagtcggca cactgtcgga
     1321 tacacctatt ctgccgatag cgtatccgcc tccgaaaaac aacgctacaa gtaatacata
     1381 tgacgaaaca ataagtgtat tctttaaaat tttcataatt tattcctcat ttcttttaat
     1441 attttttctt aaattattcc cttttgtccc atttttatac tttgcataag actttgaaaa
     1501 ataaatgttg caaatttatg caatttagga tataattaaa gtatgcaatt atctttaaag
     1561 aaagattgag gcttttaaca gccccgaaat caaagtagac atactctcac attttgtcta
     1621 caatcttaat aaaaaagata ggagaaaata ttatggctga taataacaga agaaacagac
     1681 caagctccgg atatggctca aatgataatt tcaactggga agagtatgat atgaaaactt
     1741 cgtcagcttc aggcgctccg agacgtcgca gacaagcggc atccacaaag cctacaagat
     1801 caccgcaggc aagttcttca tcatacagag aagtaccgtc aaggcgccga actcaaacaa
     1861 cttcccgcag taagaaaaag caaaaaaagc agcttactcc gaaacagatt caattcagga
     1921 aacgcttacg gcttacattc tttgttgcga tttttgtggt agttgtaatt gtatccggta
     1981 tgggcatagg aatgtatgcc gcagtatcac gtgaaattaa agatatgaat attcaaactc
     2041 ttgctttgaa ttattcttca tttatatatt acgaagatgc cgccggcaat ccgcaggaac
     2101 ttgaacaaat acaatccgat tttaaccgta tatgggtaga ttcgtcgcaa atacctcaag
     2161 ttatgaaaga cgctatcgtt tcaatcgagg atgaacgctt ctacaagcac cacggtgttg
     2221 atataaaacg tacactcggt gcaacgggta aatgggtact ttcaaaagtc ggtatcggct
     2281 cgtcaaacta tggcggtagt acgattactc aacaggttat aaaaaatata accaatgaaa
     2341 aagaaaatac accttcgcgt aaggtcaaag aaatgatgcg tgcggttgca cttgaaaaac
     2401 agcttacaaa agacgaaata cttacaatgt atctgaatat cgtatatttc gctaacaact
     2461 gtaacggtgt tgaggctgcc gcaaacactt actttaacaa agacgcaagc gaccttaccc
     2521 ttgccgaagc ggcatcaatc gcaggtat
//
LOCUS       DAWACS010000187         2535 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4282_length_2535_cov_1.652823, whole genome shotgun sequence.
ACCESSION   DAWACS010000187 DAWACS010000000
VERSION     DAWACS010000187.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4282_length_2535_cov_1.652823"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    301..310
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2044..2053
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          681 a          563 c          397 g          874 t
ORIGIN      
        1 cagaccttgt aagcagtaat gtttacaagg tttttctttt attccgccag aatgttttca
       61 aacaaaattt acctgcaaaa ttgtataggc tgctacatac tatatcagac gttaaatccc
      121 taatcaaaat cactgtggca ataaccccac tcacatattc atctttattt tgaatacccc
      181 aaaacaaaaa agaagaacca aactaatggt tcttctttta aattgtcatt cttttttaat
      241 actcgcaagc cgcaagaagt atatcgttat atgcccaatg tgtttcagaa acatcactga
      301 nnnnnnnnnn tttttcaaga atttcagtag tcatttttct atcagtaatt ctgttgataa
      361 tcgcgactgc ttcacttctt gtaagcttat tatcaggcaa gaattcatca ttctcataac
      421 ccgtaacaat tcccttttct gccaaggcat ttatatactt tttgcaccaa tcaaatcttg
      481 caatatcggt aaatttatct tcgccgtcca acggttcaat attaatatat cttgcgatta
      541 tagacgcaaa ttctgctctt gaaatcatat cttccggacg gcataagcca ttttcatcac
      601 ccgaaatata attcttttct actgcgaaat tgatataatt ctgataccat gtaccatcgt
      661 gtacatcgct gaacttatta gcatatgttt tatttacatc aaagtcaccg tcaagcaccg
      721 tcaatatctt cgccacttcc gcacgagtaa ttgtattatc cggtctgaat tgtaccacgc
      781 cgttttcttc ataaccgctt atatacggtt catgcttggt tatcggcatt tccgttggat
      841 ttggtgtttg atttccgtca ttcggtttag gcgaattcgt cggttcaaca gtcgttattg
      901 gtgtttcggt cggagtcggt gtaggagtag ctgatgatcc tttatttccg ccgcttgatc
      961 tcttctttgt tatcgttaca taatacttat gttctacacc atatttgttt ttcactgtgt
     1021 agacaacagg atttttttct gaatctgtaa agtcctgcgc accttttggc gaataatcgt
     1081 caccgtgtat ttgcaaaaca ggttcaacac tgttaagctt tgcacttgac ttcaacacca
     1141 attttacatt atcgtcttca tactctgttt caccgtactg tccgtcaaca gtcaagctct
     1201 ccaaatacgg atcatcacca acacgttcaa tatgtaccgt ataagttaca tcttcatcgg
     1261 ctgccgatga aagcgtatac ttaacaggat tgtccaaatt actgaaatcc tgtgcggtat
     1321 cggcactcgg tgatatttca tcagcgtcaa acgtgatttg cggtgtcaca gatgataaat
     1381 catagatata cggtgatgat atatatatat ccgtaccatc tatcttcgtt tcaccctctt
     1441 gcacatcagg caatgtaaat tcagttatct tacgagtcgt acgtcttgga actgtgattt
     1501 ttgtttcact tgaataattt gtatcaaaac catctataac aaattcaatt ctgtattcct
     1561 gctcactgtc agtactgtag ttagtaggaa tattaagagt tgctgttgct gatttatctt
     1621 caaatgaaat tgtttttcct tctatctttg caccaccgct tataggcact gcatacacct
     1681 ttacatcttc aaagaatata ccgttcaagc ctactgttac aagtccgccc ttaaacgtta
     1741 ccggtgaagt ataacttacc tcgttaatag taggtttacc atctcttgaa atatgtacag
     1801 tgtattctct gtccacatca tccgccgccg ataccttgta tacaagtgac tttgtttcgt
     1861 tatcaaagtt ctgctcccct tccggcgcat aactgtcacc gtcaattctg agattcggtg
     1921 taagcttgct tatgtcagta tcatactgta cttttacata aacatcctta tcctgtatat
     1981 cagcactgcc aacttgattt tgaacataga aattagttat agtcctcgtc ttacgtctcg
     2041 gaannnnnnn nnngttttta gcggaacata cgatacattt tcaaatccgt caacatcaaa
     2101 taccaatgta taattctttg aattttcagt atcatcattt tcaggcattg ttatacttgc
     2161 agacgcactt ctgaaaccgt ccgttacatc gccgacaact gcatcatggc attcaatctt
     2221 gctatttccg tcctccgata cggcataaac tttcatactt tcgttgaaaa tacctgacat
     2281 ttcaacctta acatcactgc ccttaaatgt ttcgggagca ttcgaaaccg atacacttgt
     2341 tattgtcggc atacccgaac gcttaactgt tacagtatat gtttttgaaa cacctccgtc
     2401 acctgtcacg gtatattgta cagggtttgt gaaatcctgt gttccctttg gtgtgtaatc
     2461 cttaccgtct atatcaactt taggcgtcaa cgctgttaaa tcagtcgtat aaggcatata
     2521 caacgtgatt gtatt
//
LOCUS       DAWACS010000188         2531 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4289_length_2531_cov_2.075929, whole genome shotgun sequence.
ACCESSION   DAWACS010000188 DAWACS010000000
VERSION     DAWACS010000188.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4289_length_2531_cov_2.075929"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    260..269
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1408..1417
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          852 a          361 c          630 g          668 t
ORIGIN      
        1 cgttcaggca gacgggacgt attttgaaaa tatcaaagca gccgcagaat accttgaaaa
       61 aacaacagga aacatactta taacgacggg ttcaaaggaa attgcaaagt tttcggttgt
      121 tgcggacaga gcgtttgcac gcgtactgcc gtcggaggaa tcgctggagc tgtgtaaaaa
      181 tgcaggtttc aagatgaaaa atattatttg tatgcaagga ccgttttcaa aagcgtttaa
      241 cagtgcgttg attcgtgagn nnnnnnnnnc ggcggtttta cggaaaaaac agaggcggca
      301 aaggaaaacg gcgttgagtg tattataatt aaaagaccga ccgatgaaag cggaatgacg
      361 gtttcggagg ttgaaaaaat gattttggag tggttgtaat atgaacattt ttatagtcgg
      421 tgtcggaatg ggggacacta aatatttgac gaaaattgca gaagaaaaaa taaaaaatgc
      481 tgatattata atcggtgcaa aacgtgttgc cgagccgtat gtcaacgaga aaaaagtttt
      541 ttttgagtat gaaacggata aaattaaaaa gattttagaa gaaaataagt gtgaaaatgc
      601 ggtaatactt ttttcgggtg atacgtcatt tttcagcggt gcgaaaaaat taaaagaaga
      661 atttcctaat gccgaaattt ttgcaggtat aagttgcgta tcgtattttt gtgctaaaat
      721 cggtatggcg tatgacgaca tgaacattgt aagtatgcac ggcagaaatt acaatattgt
      781 aagtgaagtc agagagaatg agaaaacatt tgtgcttttg ggtgaaaatc cttgcgataa
      841 gttgtgccga tacggttttg aaaatgccga agtttatatc ggagaaaatt tgtcatatga
      901 aaatgaaaaa atacttcatg gaaaagcaaa aggttttagg gacactaaat tagcttcgct
      961 cagtgtggcg gtaataatca ataatgaata tgacaaatgc acaaaaatcg gaattaacga
     1021 cagcgaattt gtgacgggca acgcacctat gacaaaaagc gaagtgagag cggtaagcat
     1081 atcaaagctt gaaatcaaat atgacgatat atgttacgat ataggtgcgg gaacaggctc
     1141 ggtaagcatt gaaatggctc tgctatgcgg aaaaggcaag gtatatgcga ttgaaaaaaa
     1201 agcggaggcg gctgaattga taaaacaaaa tgcacttaaa tttcatgccg ataatattga
     1261 aataatttgt gcggacgcac cgaacggtat ggacggatta ccgaaagcgg acaaggtttt
     1321 tataggcggt tcatcgggga atttgtatga aataatagaa aagtgcgatt gcaaaaaagt
     1381 ggtcgtgaac gcaataactc ttgaaacnnn nnnnnnnaaa ttatgagaag aattatgatt
     1441 gccggcacaa acagcggctg cggaaaaaca actgtgactt gtgcggtgct tcaagcactt
     1501 gtaaacagaa aaattaatgt ttcgtctttt aaatgcggtc cggattatat tgacccgatg
     1561 tttcacaaaa cggttatcgg aacggcggca tacaaccttg acacatacct tatgagcaat
     1621 gatactgtga attatttgct gaataaaaac agcggtgata ttgcggttat tgagggcgtt
     1681 atgggctttt atgacggctt ttcttttacg gaaaagggaa gtacgcacga gctttcggag
     1741 attaccgata cagatgttat tcttgttgta aactgccgtg gtatgagcct gtccgcaatg
     1801 gctttggtga aagggtttaa ggagtttagg aaaaataata ttaagggtgt tatatttaat
     1861 aatctgtccg agaaactgta cggtgatttg tcggaagaat gtcgaaaaat cggcttagtt
     1921 cctttaggtt ttttgccgaa catcaaagat gtgcagataa gcagcaggca tttgggattg
     1981 gtcaccgcag gggaaattga cggtataaag gacaagatgc atttacttgc cgattacgcc
     2041 gaaaaataca ttgacataga caagatttta gaaatatcag aaaacgatag ggacactaaa
     2101 tttacatcgc ttgaaattac aaaaaaatat aacgtaaaaa ttgcggttgc gtatgacaga
     2161 gcgttttgct tttattatga ggataatctc aatttactca gtgaaatggg tgcggagatt
     2221 gttaagttca gtccgcttga aaatgagagc gtaccgcagt gcgacggatt gattttggga
     2281 ggcggttatc ccgaactgta tgcggatatt cttgagaaaa atacggggac actgaattcg
     2341 gttaaagagt gtgttgaaag cggtgtaccg tgtattgcgg aatgcggagg gtttatgtat
     2401 cttcacgaga ttatggagga tagcgataaa aacgaacacg gtatggtcgg cataatcaaa
     2461 ggaacatgca gaaaaacaga ccgtttgcaa cgcttcggat atatggaaat gacggcaaac
     2521 agagataata t
//
LOCUS       DAWACS010000189         2515 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4314_length_2515_cov_1.856504, whole genome shotgun sequence.
ACCESSION   DAWACS010000189 DAWACS010000000
VERSION     DAWACS010000189.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4314_length_2515_cov_1.856504"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1607..1616
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          864 a          494 c          518 g          629 t
ORIGIN      
        1 tgtcgatact tataaatcac aaagacgcgg aggaagtggt atttcaggtc ttacaactcg
       61 tgaggaggac ttcgttgaac acctgtttac aaccacaacg caccacacat tgttgttctt
      121 tacaactcac ggtgttgtgt acaagctgaa aggttatcaa attcctgagg caagcagaca
      181 ggcaaagggt acggcgattg taaacctact tccgcttgaa aatgatgaaa agatttccgc
      241 aatgattcct attaaggact tcgaggacgg caaatacctt acattcatca ccaaaaacgg
      301 tattgtcaaa aagacaaacg ttatggatta ctcaaaaatc cgtaacggcg gacttcgtgc
      361 gattgacctt gacgaaaacg atgaacttat cagagttaaa cttacagaca atactcaaga
      421 tattattatt gctacacatg acggttatgc aatccgcttt aacgaaactg aggtacgttc
      481 aacaggccgt acaacaagag gtgttatggg tatccgcctt cacaacggcg actatgttat
      541 cggtgcgtct gttgcactgc ccgacagcca gcttcttact gtcacagaaa acggttacgg
      601 caagaaaaca ccgcttgacg agtatcgtat tcagtcaaga ggcggtaaag gtattttcac
      661 ttatcgtatt accgagaaaa caggcaaact tgccggtatg aaaactgtca cagataatga
      721 cgatattatt cttatcacat ctgacggtgt tattataaga atgcacacag atgaaatttc
      781 ttcatacagc cgtcaaactc aaggtgtaag agttatgcgt cttgacgacg gtgtaagtgt
      841 tatgagtatc gcacgaacag aacgtgaaga agaaactgat gaagaagcag aaaattctga
      901 agaagttatt gctgatgata caaccgaaac tcaaattgct gacgaaagcg aaacagaagg
      961 agatgtcgag taattatgaa aaaatcaatt agtgttatag ctataattct atcaatggta
     1021 atggtgcttg cgtcatgcgg cacaaaccaa aacggcaaag atgaagagcc cgtttcaaca
     1081 cccgatccgg aacttgtaaa aaacacagtc aacgccacaa tagaacttga agacggtgac
     1141 gaaattatgc ttgaattata tccggacctt gcacctgata cggttgaaaa cttcgttgaa
     1201 cttgcagaag acggttttta tgacggcaca atattccaca gagttattga agattttatg
     1261 attcaaggcg gcggctatga cgaaaatctg aaagagcaaa aaactgagtc tatcaaaggt
     1321 gaatttgcaa agaacggttt tgaaaacaca ctttctcaca ccagaggtgt aatttcaatg
     1381 gcaagagcac aggattatga cagtgcaaca agtcaattct ttatcgttca aaaagatgcg
     1441 acttaccttg acggtcaata tgccgctttc ggtagagtta cagagggtat ggatatagtt
     1501 gatgaaatcg catctgtaaa aacaggtacg gttgcctcat caaatatgga ggacgtccct
     1561 atcgagccta tcgtgattga cactatcacc attgacacat ctgtatnnnn nnnnnngcgg
     1621 taaatcaagc aaaacaaagg ctacttcaaa gccgtcaaaa gataaagaca cagacaagaa
     1681 ctcaaaagat actgatgatg aagacgaaga tacatcatct tcaaagtcat ctaaatcatc
     1741 atcaaaatca acaactgctc cgaaatcaac acctaagtca aactcaagtt caaaatcgag
     1801 cagttcaaat tcttcatcaa gcagtaaatc aagcaattca aatgatgaca aaaacaacga
     1861 tgatgaatta agcgaagatg aacttaatga cattcttcca ttcttacagt caggctcaac
     1921 agagtcaaat tcttaaaaag caaggagata aattatgaat attgaaatcg aaatggaaaa
     1981 cggcggaatt ataaaagcag agctttatcc ggacgttgca ccaatcacag tagaaaactt
     2041 tgtttcacta atcgaaagaa acttctttga cggtcttatt ttccaccgtg taattccggg
     2101 ctttatgatt caaggcggcg cttttacggc agacggtcag cacaaagaaa cagattcaat
     2161 caagggcgaa tttgacggta acggtgttac aaatccatta aagcatacaa aaggtgtgct
     2221 ttcaatggca agaacaatgt tcccgaacag tgcgtcaagt caattcttca ttatgcacga
     2281 ggatgcacct catcttgacg gtcagtatgc cgcttttggt aaacttgttg aaggtgaaga
     2341 tgtattagac agcattgctt caattgatac tgactggagt gacagaccta gaactcctca
     2401 ggttatgaag actgttactg ttgagacatt tggcgaggaa tatccagaac cagagaagtt
     2461 ataatttaat cttattaaac aaaaccggcg gatacagtca atagctgtac ccgcc
//
LOCUS       DAWACS010000190         2481 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4391_length_2481_cov_1.558945, whole genome shotgun sequence.
ACCESSION   DAWACS010000190 DAWACS010000000
VERSION     DAWACS010000190.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4391_length_2481_cov_1.558945"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2107..2116
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          828 a          503 c          486 g          654 t
ORIGIN      
        1 tttcaacgct tatccaaacg cttgcaatcg gtcgtctgca tattatcggc gatatttttg
       61 accgtggtcc gcgagccgat ataattcttg acacactgac gaagtaccac agtgtcgata
      121 ttcaatgggg caaccatgat gttcagtgga tgggtgccgc tgcgggtagt ttggcttgta
      181 ttgcaaatgt gcttgctatt tccaccaagt attcaaactt tgactgcctt gaggacggtt
      241 acggaattaa tatgcgtccg cttacggtat ttgcacttga aacttatgcg gacgatccgt
      301 gcgaatgctt tatacctcga aatccgaata tggtttatat ttctcagcat gacgaaaact
      361 tttgggcaaa ggtacataaa gctatatctg taattcagtt taagttagaa ggacaaatta
      421 taaaacgtca ccctgaattt aatatggaca accaccttat gcttgacaaa atcaattacg
      481 aaaacggaac aattatgctt gaaggcaaag aatacaaatt aaaagatacc aacttcccta
      541 caatcaatcc cgaaaatccg tttgaactta cagacgccga aaaagagctt atgaatttat
      601 tgcgttcatc gtttttacgc agtgaaaaac ttcaaaatca cgtcaaattc ttatacgaaa
      661 aaggcagtat atatctgaca ttcaacaaca atttgttgta tcacggctgt ataccgatga
      721 acagtgacgg tacttttacg gaagttacac ttttcggtga aacggtaagc ggcaaatcgc
      781 tcatggacaa agccgaacag cttgcacgtg acggttattt tgccaaaaac ggttccgaag
      841 aaaaagaata cggcaaagat tttctgtggt ttttatggtg cggttgctac tcccctgttt
      901 acggcaaaaa taaaatgact tcgtttgaac gatattttat tgatgatgaa agtacatggg
      961 tggaaattaa agacgaatat tatcacctta tcgaaaagcc gagtgtatgc aacaaaattt
     1021 tatgtgagtt cggaattgac ccgaataatt tttctcacat tataaacggt cacgttcccg
     1081 taaaaatcaa gaaaggcgaa agtccaatta aagcaaacgg caaactttta gtcatagacg
     1141 gcggtctttc aaaggcatat cagaaagtta ccggacttgc cggttacaca ttgctgttca
     1201 attcacacgg tttgctttta agtgcgcatg acgcatttga atctatcgaa agtgccataa
     1261 aagaagaaaa agacattcac tcaacgcttg acgttgttga acttgctccg aaccgtttac
     1321 ttgttgagga cactgacgca ggtaaatcgt tatcgaaaaa aatcaccgac ttaaaagcgc
     1381 ttgttgacgc atatttaaaa ggaaaaataa aaacacatta aataaagaga ggaaatccac
     1441 aaatgaatta cgaaaaagaa ccactgcata tttcaacatc cgtacccaac ggcacttatg
     1501 aagtaactgt cacagttacc gcacacgaag atattatttt cacaatactt tcgcagtcac
     1561 gccgttttat ggcacaagat ataaatcttg gcaagggaga aagctcagac attactttca
     1621 atgtaagcgt atgtgactac cacaaaaaca acgaagatta cacaaacgta aacggagttg
     1681 aaattgatat tatgtgcgac ggtgatttta ccgcactttc cgcagtatcg cctgtcaata
     1741 ttccgacagt ttacatcgcc ggtgactcaa ccgtcacaga ccagcctgcc gaatatccgt
     1801 acaacgctgc ctctacatac tgcggttggg gtcaaatgtt tccgcaattt tttaacacgg
     1861 gcattgcggt tgaaaaccac gctcaatcag gttctacaac cgaagatttt aaaaatgtga
     1921 atttcaccgc attcaaagac aaaatcaaaa agggtgattt cctgattatc gagttcggtc
     1981 acaacgacca aaaaatagat actcttgacg cgttcggcgg atacacggag aacttaaaat
     2041 attttgtaaa ctttgtccgc gaaaaaggtg caaatccgat aatatgttca ccaatcaaca
     2101 gaattannnn nnnnnntata ttccaagagg acggaacact tcttaacttg ctcggtgatt
     2161 acagaacagc agttaaaaac gtatgtgacg aaatgaatgt accgtttatc gacttgtggt
     2221 cacgcacaac cgaatttttt gaggcggcag gagcggtaaa agcttgggat tatttctggg
     2281 gcaacggcac tgaccgcgat tacacccaca caaacgatat cggcggcaac atcgttgcac
     2341 gatttgttgc aaacgaaatg attaaaaaca acgttcagcc gatagcagac ttaatcaaaa
     2401 aagatatgat taacgtcgaa atgccaaaac acgattcaag tgtcaaaccg caaaattcaa
     2461 aagaaatcga acacttaaaa a
//
LOCUS       DAWACS010000191         2481 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4386_length_2481_cov_2.385408, whole genome shotgun sequence.
ACCESSION   DAWACS010000191 DAWACS010000000
VERSION     DAWACS010000191.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4386_length_2481_cov_2.385408"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          834 a          418 c          562 g          667 t
ORIGIN      
        1 ggttacatta gcgtgcagcg aatgcaagca gagaaactat aatacaatga agaataagaa
       61 gaatgaccct gacagacttg aaatgaacaa gtattgtaag ttctgcagaa agcacactct
      121 tcacaaggaa accaagtaat tttgattgag taaggatgtg taaaaatggc tgatacaaat
      181 ttatctaaag caaataagcc aagctttggt acaagagtca agagattctt taaggatact
      241 aaggctgagc taaaaaaggt cacatggcct gacaaggaac agcttattca taacacaggc
      301 gtaattattg tgtttatctt aataattaca atcatcttgt ccgtacttga ttttggtttt
      361 gcaaaattgt tccaactatt aactaacata ttataattca ttaaaagaat tgttagggaa
      421 ggagaacggt tatggcagaa gaagcaaaat ggtatgtggc acacacatat tcgggttatg
      481 aaaacaaagt aaaagccaat attgagaaat ctgttgaaaa ccgtggtatg caggatttaa
      541 ttcttgatgt caaggtgccg accgaagaag tcgttgagga aaacggtgat aagaagaaag
      601 tagttcagca caagattttc ccgggctatg ttattatcaa gatgataatg acagatgaaa
      661 gctggtacat tgtcagaaat acacgtggtg ttacaggatt cgtgggaccg ggttcaaagc
      721 ctgttccgct ttcggacgaa gaagttgaac gcatgggtgt tgaagttatt cgtatgaaag
      781 atattgattt taccgtagga gatcttgttt caattaaatc aggccctatg gaaggctttt
      841 cgggtaaagt tacgagcatt aacaatgaaa cacgtaagat taacgtcgct gtttcgatgt
      901 tcggacgtga aacccctgtt gaaattgact atacacaggt tgaacatatg gaatagtcaa
      961 tcaaattatt ttgatataga cagcaaatgt tgtttataat atccctctgg taacagtggg
     1021 agggctttat gcccgcaatt accacattca tatagcgata tgctatgaaa aattcaagga
     1081 ggtggccatt atggcacaaa aagttgcagg ttatattaaa ttacaaattc ctgccggcaa
     1141 agcaactccg gcaccaccgg ttggtcctgc tctaggtcaa cacggcgtaa acattatgca
     1201 gtttgcaaag gaatttaacg aaaaaacagc aaaggacgca ggtctaatta tccctgtagt
     1261 tattacagta tatgctgacc gttctttctc attcgtaaca aagacaccac cggctgcagt
     1321 tcttatcaag aaggcttgta agatcgaaag cggctcaggt gttcctaaca agacaaaggt
     1381 agctacaatt tcaaaggcag atcttcaagc tatcgcagaa cagaagatgc ctgaccttaa
     1441 cgctgcaagc gtagaggctg ctatgagcat gatcgcaggt acttgcagaa gtatgggcgt
     1501 tctaatcggt gactaatttg ttaagagaag tgggagagat ataaaatctc gtttaaccac
     1561 gaaggaggat acgaaatgtt tagaggaaag aaatatcaag atagcgtaaa gcttgttgaa
     1621 aaatcaaagt tttacgatac aaatgaggct atggacctta ttacaaagac agctaaggct
     1681 aagtttgatg aaactgtaga agctcacatc agacttggtg ttgactcaag acacgctgac
     1741 caacaggtca gaggtgctat tgtacttccg catggtacag gtaagacttc aaaggttttg
     1801 gtattcgcta agggtcctaa ggctgacgaa gcaaaggctg ctggtgcaga ttacgttggt
     1861 gatgctgact tagtagcaaa aatccagggt gaaaactggt tcgatttcga cgttgttgtt
     1921 gcaactcctg atatgatggg cgttgttggt cgtcttggta aggtacttgg tcctaaggga
     1981 cttatgccat caccaaaggc aggcacagta acaatggacg ttacaaaggc tgtaaacgaa
     2041 attaaagcag gtaagattga gtacagactt gataagacaa atatcattca ctgcccaatc
     2101 ggtaaggcat cattcggtgc tgaaaaactt caggaaaact ttaacgcact aatgagtgca
     2161 atcgttaagg ctaagccggc tgctgctaaa ggtcaatacc taaagagcgt tgttgtagca
     2221 tcaacaatgg gacctggtat aaagattaat gcagctaaaa ttggctaatt gattttaata
     2281 gcaataaaaa accgctcaca atatgagcgg tttttgcatt ttgttaaaat ttttttaatg
     2341 aaaatcaaaa aaactattga caagctgttt aaattatgct atactataaa agttgaaaat
     2401 ccgtagacag tcggcggctt gcaatgtaca ttgcagacat aagtcctacc gagggtgttt
     2461 tagtaatgac tattacaaag c
//
LOCUS       DAWACS010000192         2480 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4393_length_2480_cov_2.439175, whole genome shotgun sequence.
ACCESSION   DAWACS010000192 DAWACS010000000
VERSION     DAWACS010000192.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4393_length_2480_cov_2.439175"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    689..698
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          760 a          539 c          414 g          757 t
ORIGIN      
        1 accgttgtac gaatacattt cgcctttttc gtcggttgca tacattgttg ttttgatatg
       61 cttatcatag tcaatgtcgt accattcaat cgtgtcgggt tctgtcgcca catctgaact
      121 tactctgtca taaagataac cgagtacgcc ttgtatctta cccgtttcgt aatcatcttt
      181 atttaccgct tgtccgccct ctttattcat aaacgcactg taaccgtaat acgcgccgag
      241 ttgcgtccag tggtggtcgg ttctgaaata tgtgtactca tttgaatgtt cggacagcaa
      301 accgtatgcg tcaacagtac ccaccctgct gtcaagcttt gaataaatac tgtctatcgt
      361 atccttttga ctgtcctgca aattcttata tataggttct ttaaattcaa gctgtgtcgg
      421 aattaccata ctgaaaagtt ttatattttc gggaagtttt tcggcatagc gatttatcgc
      481 gtcggcatat tcctgctctg ttttatcgtt tcttataaac atctccataa tagtgttatc
      541 cgcaacaaga agagtttgtt tttgagttgt acccgttcct atatctttat ttgtggaaat
      601 aattttacct gttttcggta caaatcctct tttttcgtcc aacgtttttg aaaaatcggt
      661 aatttgtgaa cgataagcaa tattatccnn nnnnnnnngt ttcaaattca cccgaaaaca
      721 ctgtttcctc ctttacgggc ggaatagttg ccatcactct gttttcagat tttatacttt
      781 cctcgtccgc cggaagtgcg agtacaacga taaaagctgt cagcataaga agaaatgcac
      841 cgattgtaag cttatttctc atattcgtca cctttcctta aaatctgaaa tacaaaaacg
      901 gattatatgt ttgtcccaca agcaaaacaa agcacactcc aagtccgaca ataaccaaaa
      961 ccggctccga tattgccgca aatacctttg attttttgca gaaattcttg tgtataattg
     1021 tcggtatcgg agttgacgct attacgcaaa ccgcaataag ccatgcattc gtacagaatg
     1081 ttgacaccgc cgtcaagtcg tacaatgccg tacccacacc gaacgcactc tttatgaatg
     1141 tccaaagtgc gtttaagtcc gtaaaataga acagcgccca accgagtatt acgaagaaca
     1201 tagtgtatat atagcagaac ggggcgggaa ttttttgaag tttcttttta aacagtgttt
     1261 tttcaataaa caaaagtaca ccgtaaaaca atccccaaaa tataaaattc cagcttgctc
     1321 cgtgccaaag tccggttaaa agccatacta tgaatatatt ccgtattgcg tggtatctgt
     1381 taccgccgag cggtatatag acgtaatctc tgaaaaatgt tgacagcgat atgtgccacc
     1441 ttctccaaaa ttcggttata cttcttgaaa tgtacggata ttcaaagttt tcaccaaagt
     1501 gaaaacccaa cattcttccc aagcctatcg ccatatcact gtaacccgaa aaatcaaaat
     1561 aaatctgaaa tgtatacatt attattccga gccatgatga cgcaaccgta agtcttgtac
     1621 tgtcaagcac cattgacgca accgcacccg aactgtttgc aagcattact tttttgcaca
     1681 tacctgtggt aaatcttttt acgccgtatg caaaatcatc agccgtaaca cgtctgctgt
     1741 tcagttcgct cgcaaccgta ctgtaccgca caataggtcc tgcgacaagc tgcggaaaca
     1801 ttgaaacgta tgccaaaaat cccaaaaacg atttttgcac tcgcgttttt cctctgtaca
     1861 tatctatcgt atacgacagc gtttgaaatg tgtaaaacga tattccgata ggcagtttga
     1921 aattcggatt agggattgac gtgtgtaaaa acgtgttgat gttttcaata aaaaatcccg
     1981 aatatttaaa cattgcaagc aagcctaaat ttataacaag tgacgaaaca agtgcaatcg
     2041 ttgcacctgc cctcgcatag tatttatcaa ttatacgtcc gtttatataa tccacaaacg
     2101 cactgaatat caaaagcact acccatatcg gctcacccca agcgtagaaa aaaagcgagg
     2161 cgataatcag tactgtattt ttatatgtaa gatttttcga taaattatat gcaatcagca
     2221 ctatcggcaa aaatatgcac ataaacatta aacttgaaaa taccaaaaga acaatcctcc
     2281 tgtttttttc attacaaaaa cgattatact acattaccgt cgtataagtc aatatcgcat
     2341 aataaattcc atacaagaaa aagctgtctg aaatttatca gacagcctca gactgtcgac
     2401 aaattgtccg gaacgtgcaa tatattattt ttatatttta aagtttaatt tatagctata
     2461 aaaagcaaca ggcaagccta
//
LOCUS       DAWACS010000193         2478 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4398_length_2478_cov_2.042097, whole genome shotgun sequence.
ACCESSION   DAWACS010000193 DAWACS010000000
VERSION     DAWACS010000193.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4398_length_2478_cov_2.042097"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    286..295
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          838 a          400 c          503 g          727 t
ORIGIN      
        1 gcgtaggtcc tatgactatc gctatgctta tgaaaaattc agttaaagcc gcaatattaa
       61 ataaatcgaa ataatgacac aacaaagcag gcaagccgta aaataggctt gcctgtgatt
      121 ttattgttct tttttaggaa tagttacgta cacatcagta ccgacaccga ctgttgattc
      181 aacgtgtatt ccgtagctgt caccgaatat aattttaatt ctttgattta catttgaaag
      241 acctatatgc gatttttcgt ttgtaaaaga tgtctcggaa agagtnnnnn nnnnncatca
      301 agatcgtctt tttcaattcc gacaccgtca tcactgacta tgaggataat attgttgtcg
      361 tcacacaagc ctttgatttt tattaagcct ttatcatttt tctgctttat tccgtgataa
      421 acggcatttt caataatagg ttgcaaacag atttttataa tcgtatatga taaaatcgat
      481 tcgtcaatat ccctttctat atcaaacaaa tcaccgtaac gcagtgcaag aatattaatg
      541 tattctttgg tgtattttat ttcgttttcg atagaaacaa aataaccgga agtaagtgta
      601 tttctgaaaa agcttgcaag attaatcagt gatttagata ccggattttc tgaattggac
      661 atattataag ccatccaatt aattgtttca agcgtattgt aaagaaaatg cggattaatc
      721 tgtgtttgga gcatatcgta ttgtgatttc ctcagaagtt tcatacgctc ttctaaaatt
      781 tcagccattt ttgtcttgtc gttgatatgc gtctgtatgc tgtttattat atacataagt
      841 tcgtttttat cttcttcctc aatactgtcg gcaggaggct gtgaattatc aaggaatgaa
      901 ataatttcat taagcggact gtaacttctg acagacgcaa cataagcaag tatgaaaata
      961 ataatcataa gcaaaagtaa aataccgata atttgcaatt tcatagttga taacttatgc
     1021 tcgtaaagtt caagaggata tgcacttata tactatacac cgaacgatgt tataatatct
     1081 ccgcaatata tatacgaaat acaagatacg aagaacattg tagaacttca gcaaaaagaa
     1141 catacaaccg gagttgtcaa aagagcatac ggcaatgaac ttgtattcgg tgaaaattat
     1201 gacagcggag gtgcaggaat aataacgact gtcgatgatt atgccaagtt tgcggcggct
     1261 ttggcaaata gcggaacagg acttaataat aatcgtatac ttagcagtgc cacagtaaaa
     1321 ctgatgaaaa caaatcaatt aaatgaggca cagcgtaaaa ctatgaattg gcgacgtctt
     1381 agaggttacg gttacggatt aggtgtaaga acgcttatag ataaagccga aagcggttca
     1441 aacagttcaa tcggtgaaat cggttgggga ggtgcggcag gtgcaacaat aattgccgat
     1501 acagaagaaa aagttgcact tttctatgca caccatatgt taaacccaca agaagaatat
     1561 tatcagccaa ggctgagaaa tgtattatat tcatgcttat aattaattaa aaacagaaag
     1621 gaagtaataa aaatgaaacc aagaatagga ataagaccga caatagacgg tcgttggggc
     1681 ggcgtcagag aaagccttga agaaaaaaca atggctatgg caaatgccgc aaaggaactt
     1741 attgaaagta atgtgcatta tcaagacgga acacctgttg aatgtgtaat ttcaccttgt
     1801 acaataggtt caggcgctga ggcggctaaa tgcaacgatt atttttcaac acaaaatgta
     1861 tgtgcaacat tggctgtaac accatgctgg tgctatggtt cagaaacaat ggacctaaac
     1921 tcggatacag taaaagctgt atggggcttt aacggaacgg aacgtcccgg tgcggtttat
     1981 cttgccgcag taatggctgc atttgcacaa cgcggacttc ctgcattttc gatgtacgga
     2041 cacgatgtgc aggacattga tgataacaca gttcctgaag atgttgccga aaaaatgatt
     2101 cgttgggcga aaggtgctgt tgcggtcggt caaatgaaga acaaagcata tgtgaacctt
     2161 ggcagtgtcg caatgggtat tgcaggttca tattgtgata tgtcatttat gcaaaagtat
     2221 cttggtatac gagctgaatt tgttgatttg acagaaattt tgagaagaat tactcttggt
     2281 atatatgata aagatgagta tgaaaaagca tataaatgga taaaagaaaa ctgcaaagaa
     2341 ggctttgata aaaatgcagg taaaaacttg cctgacatta ttacaaaatc gaaagtcgtt
     2401 tcggcagata aggattggga atttatcact aagtttacgt taatcgtaaa tgatattctt
     2461 tacggaaatc caaaactt
//
LOCUS       DAWACS010000194         2467 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4417_length_2467_cov_2.600746, whole genome shotgun sequence.
ACCESSION   DAWACS010000194 DAWACS010000000
VERSION     DAWACS010000194.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4417_length_2467_cov_2.600746"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          724 a          472 c          492 g          779 t
ORIGIN      
        1 attgtcaatt cccttgattt ttcgatataa tagtgttaaa atagacaaga tatatctaaa
       61 tatatccgtt ttcggtaaac aaatattttt atatttgttt tcaaaaggag aagcagggtg
      121 aaattccctc acagtcacgc taccgtacag tcggatcgcc tgccgaaaat cgagcgcatt
      181 tgtgcctacg aacgatgggt caatggcgga atcattcaga ttttaagcca taggatttac
      241 ccaatcccta cggctttttt tggtatgtga aaatcacaag ttttctacac tcccaaaaag
      301 tttcataact gatttaagtg gtcacttgtg tgctaaaaca tatttcataa gaagaaaagg
      361 agaaaaattt atgtctttag caaacaggaa aacaaaacta ttggtggcaa tgctttcact
      421 actgcttgtc attcctatga caaacgcatt cgcaatgcac attatggagg gttatcttcc
      481 tccgaactac tgtattatat ggggagttgt atgtataccg ttccttatcg caggcggtat
      541 cagtatgcaa aaacaagcaa agaagaacaa caaaacaaaa atacttcttg cacttgcagg
      601 agctttcgtt ttcattcttt catcgcttaa aatcccttca gttacaggaa gttgttcaca
      661 tccgacaggt acaggactcg gtgcaattct tttcggaccg tcgattatgt ctttactcgg
      721 tataatcgtg cttttgttcc aggcacttct tcttgcacac ggcggactta caacacttgg
      781 tgcaaacaca ttttcaatgg cgattgccgg accgttcgtt tcattcggta tatacaagct
      841 ttgccaaaag cttaacgcac cgaaaggtct tgcagtattt cttgccgcaa caataggtga
      901 tatattcact tatgtggtaa catcatgtca gcttgcactt gcattccctg accaaaactt
      961 ctttgcatca cttgtaaagt ttatgggaat cttcgcggtt acacaagttc cgattgccat
     1021 tgcagagggt attctgtctg ttatgatatt caatgtattg gctaagaact gtacaaacga
     1081 acttaagcag cttaatgtta tataaggggg tatatattaa tgaaactttg ggttaaaaat
     1141 acaatattgg cagttatact tgttcttata attgccgtac cgctactgtt tatgggcggt
     1201 cacgaatttg gcggttctga cgaccaagca tcagacgcca taagtgaatt ggacgaaagt
     1261 tacgagccat ggtttgagcc gttctgggag ccgccgtcag gtgaaatcga gtcacttttc
     1321 ttctgtcttc aggcaggagt gggcgccggt attgtcggct ttgtacttgg cagagtaaca
     1381 gctaaaaaga aaaatgataa ttgataaatt agcatataat tcaaaactca gaaatattgc
     1441 acctattgca aaacttctgt tttcaatgtc ggtgcttgtt atctgcatat gggcaaacag
     1501 ctttttggta agcgtattta ctgcgcttac tatgctgttt cttattattt ttataggaaa
     1561 aacatcgtat aaaaacgtat ttcatcttat gactgtcccg atagttttta ttattatggg
     1621 tgcggccgca atagcaattt caatcggtca aaattcaggc gatatgattt tttcactgca
     1681 tttcggcaat acttatttcg gcgtgagcca taccagtctt ttatcggctg ttcgtgttat
     1741 gattaagtgt ttcggtgcgg taagctgtat gtattttctt tcacttacca cgcctatggt
     1801 cgatttattc actttgctca gaaaaagcat tataccgaat tttataatcg aaattgcgga
     1861 actgatttac agatacattt ttgtgctgtt tgacgtatcg cacagaatac acaccgctca
     1921 agacgcacgt ttggggtaca gcaatcttcg tgtaagctac cactcaacgg cacagcttgc
     1981 gtcaaacctt tttatacgtt cctttaatca agccgaaaaa acgtataccg ctatggagtc
     2041 acgcggttat gacggcgaaa tcaacgttat tatgccgaag attaatcatt cggtcaaatt
     2101 taacgtattt gcggtcatat ttgtcgtaat tacaacagcg attgcgatat tttcaagaaa
     2161 ggcgggaatt tgatgaatat tatcgaaaca gaaaaccttt catataaata tgatgaaagc
     2221 cattttgctc tttcggattt gagcattggc tttgaaaaag ggaaaatcac aacaattctt
     2281 ggtgcaaacg gagcaggcaa atcaacattg tttctgaata tgaacggaat tttaaaaccg
     2341 cattcgggga cagtaaaatt tatgggtaaa ccgataggtt attcaaaaaa ggaaatcaga
     2401 gaattaagaa aaagtgtcgg aatagttttt caagaccccg acgaccagct tttttccgcg
     2461 tctgttt
//
LOCUS       DAWACS010000195         2465 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4423_length_2465_cov_2.464730, whole genome shotgun sequence.
ACCESSION   DAWACS010000195 DAWACS010000000
VERSION     DAWACS010000195.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4423_length_2465_cov_2.464730"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    558..567
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1943..1952
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          804 a          406 c          569 g          666 t
ORIGIN      
        1 gtcttgtata cgcaattctt tatgcgttgc ttatactatt cttcacatat ttctattctt
       61 caattcagtt caatccgatt gaacttgcaa ataatatgaa gaagagtgga ggatacattc
      121 cgggtatcag acctggtaag ccgacaagcg attatatagg aaagatttct tcaagattaa
      181 atcttgtagg tgcattcttc cttgcaatta ttgcaatcct acctgtaatt gtcggtgcga
      241 tattcaatgt aacggcactt caaatcggcg gtacatcact tctaattatg gaaggtgttg
      301 ctcttgaaac tgtcagacaa attgaatcac aaatgacaat gcgtcattat aaaggtttcc
      361 ttgaataatt tcaggaaagg cggtatttag tatgaatttg gtattaatgg gccctccggg
      421 ggcaggaaaa ggcactcaag gcgaaatttt atctaagaga ttggatataa acacaatttc
      481 aacgggtgtt atgctgagaa ccgccatcaa ggaacagtca gaaatcggaa agattgctga
      541 aaagtatatc aatgatgnnn nnnnnnnctt gttccggatg acgtaattgt ttctatagta
      601 aaagaaagat tgcaaaagcc tgattgtgca aagggattta tccttgacgc attccctcgc
      661 acaacagctc aggctgaggc attgacagaa tcgggcgtaa aaattgataa agtgctttct
      721 cttgaagttg ccgatgaggc aataatcgag cgtttgtcat caagacgtga atgctcaaag
      781 tgtggagcac cgtacaatat catatcaaac aagcctgaaa ctgaaggaat atgtgataaa
      841 tgtaaaggcg aacttattca gcgtgccgat gatgttcctg aaacaatcaa gaacagactt
      901 aatgtttatc acgaacagac agagccgata aaagcgtatt atgaaaagct tggacttctt
      961 gttactgcaa agggcgagga taagcttgaa gatacaacaa gcaatgttgc taaggctttg
     1021 ggacttgagg tgtgagttat gataactatt aaatcagcaa aacaagtcga aaaaatgcgt
     1081 gcgtcagcaa aagttacaaa aggtgcgctt gagcttcttg agaagcacat caaaccgggt
     1141 gtaacaacgg cgcagcttga taaaatcgca tatgatttta ttatatcaca aggtgcaaaa
     1201 cctaatttcc ttaattataa cggtttcccg ggaacaattt gtgcgtcaat caatgacgag
     1261 gttgttcacg gaattccaag taagagagcc attttgaaag agggcgacat aatcagcatt
     1321 gatatgggcg ctatacttga cggatggcac agtgatgccg caagaacatt tggagtcgga
     1381 aagatttcgg acgaggcaca aaatcttata gacgttacac gtcaaagttt ctttgaggga
     1441 attaaatatg taaagcacgg tgccaaatta ggcgatgttt cgtcagcaat tcagcagtat
     1501 gttgaatcgc atggttacag cgtagtgcgt gaccttgtcg gtcatggaat tggtcgtaat
     1561 cttcatgaag atccgagcgt accgaatttt ggcaaagccg gtcatggcgt aaagcttgcc
     1621 gcaggtatga caattgctat tgaacctatg gttaatgcag gtgattatca tgttgcggta
     1681 cttgatgatg attggacagt tgtaactgat gacggaagtt tgtcagccca ctatgaaaac
     1741 accgtattga ttacaaaaga cgggtgtgaa atattaacac tttaggagta ttatatggaa
     1801 attatagagg gttcaatcgt gcgttccatt gcgggacgtg acaagggtga tttgttcatc
     1861 gtcctttcaa gagaaggcga ttttgtttat cttgcaaacg gtgaattgcg aaaagtggat
     1921 cacccgaaaa agaaaaagct cannnnnnnn nnaagcacct tcagggtaca aactctgttt
     1981 cggattttgt aagtaacaaa cttgcaactg cgggtaaggt tacaaattca gaggtgcgaa
     2041 aagcattagc ggagtttaat taaacctgaa agcgtatcta aaggaggttt ataacatatg
     2101 tctaagcaag atgttataga agttgaaggt aaggttgttg aaaacttgcc taacgcacag
     2161 tttagagttg agcttgaaaa tgggcaccag gttacagcac atatttcagg caaactgaga
     2221 atgaatttca taaaaattct accgggagat aaggtaactc ttgaaatgag tccttatgat
     2281 cttgataagg gcagaattac atggagaagt aaataattta cttcggtcag ataagtgacg
     2341 acaaggaggt aatagatatg aaagtacgtc cgtcagtaaa accaatttgc gaaaagtgca
     2401 aaatcataaa gagaaaaggc aaagtaatgg ttatttgtga gaatcctaag cacaagcaga
     2461 gacaa
//
LOCUS       DAWACS010000196         2457 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4438_length_2457_cov_2.017902, whole genome shotgun sequence.
ACCESSION   DAWACS010000196 DAWACS010000000
VERSION     DAWACS010000196.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4438_length_2457_cov_2.017902"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          835 a          395 c          542 g          685 t
ORIGIN      
        1 ttttgcgatt gcctcagctt ttaaatcaaa gtaaagctga gtaaatgatg ccgatatata
       61 cggctcaagg aacaaaaatc ctataccgaa tgtaatacag cttagaatta tccaaccgat
      121 aaacgaaagt gagaataaaa atattctgcc tttgtttccg tcggtaagcg attttgtgac
      181 ttcaaatgct ctgcgtctgt ccatagccgg attttcggca agaatataag gaatcattga
      241 gtattggaat gatttgataa ttcccggtat gatgaacaac agcgaccaca aaaatgtata
      301 aatcgcaaca aacacattaa taccgattac gttgccgaga ttgtttttat aagggaaaaa
      361 caaatcgtat acattaggtg cgttgacagt tgcctttatg aaaaatctgc acgatcccac
      421 gcttagaggg aatgtgacaa aaagagtgat taatatttga agtacggctg atattgatgt
      481 acctgccgaa ccggcgtccg aattaaccat attagtcact gtggcaaccg aagttccgag
      541 tgatgtgata agtgaaacca atatacatac tccgaatgaa taccagtagt ttgactttaa
      601 aacgtcttta gctcttgttt ttagttctgc aaaagtagtt ttatacatat tgccaagacc
      661 tcctttatat ttaagatata tcgcataaag tatatcatat gcaaaatatt ttttgtagta
      721 ggaaattcat aatgtaaagt aaacttaata aaatatactt gtttatacaa agaaaatgtt
      781 atataataaa aaaagatata tattattgta atttacaaag gagatatagc gatgagtaag
      841 agtgtcaatt tatatgtagt ggtaccgtgc tataatgaag aagctgtttt gcatgaaaca
      901 tcgaagcaaa tgcttgaatt attcggtgaa atgaaaaatg acggacttat aaatgacaac
      961 agcagaattg tttttgtaga tgacggttct aaagataaaa catggagcat aatagatgaa
     1021 ctgactaagg ctcatacaga aatatcggga ataaagcttg ccaagaatgc gggacatcaa
     1081 aacgcacttt tcggcggcct tatgacagtt aaagacgact gcgactgtgc aatatcaatt
     1141 gacgcagact tacaggacga tatacacgta atacctgata tggtgagaaa cttcattgac
     1201 ggatatgatg ttgtttacgg tgtaagaaac aaacgtgata ccgatacatt cttcaaacgc
     1261 acaacggcga taggatttta taaattaatg cagtttatgg gcgttaatat tgtattcaat
     1321 cacgcggatt acagattgat gagcaaacgt gcgcttgacg gattggcaca atttcctgag
     1381 agaaatctgt tcctaagagg aatggtgcct cttgtcggct atcgctcaac aaatgtgtat
     1441 tacgacagaa acgaacgctt tgcgggcgag tcaaaatatc cgttaaagaa aatgctttca
     1501 tttgcgtttg acggtataac ttcattcagc ataagcccaa tcagaatgat ttcggcattt
     1561 ggtgcgattg tgtgcgtatt tgcgttcatt atggcgatat acgcacttgt gcagaaaatt
     1621 ataggtcaca caggtgcagg ctgggcgtca cttatggttt cgatatggtt tataggcggt
     1681 gtacagcttt tgtctgtcgg acttataggc gagtatatcg gtaaattgta taaagaagtt
     1741 aaacgccgtc caagatatat tattgaagca tatgtaaatg aaaaagagaa agaggacgat
     1801 aaataatgat atatgatgtt gcaataatcg gcggaggccc tgccggaatg tcagctgcta
     1861 tatatgccgc aagaggcggt atgaaaacgc ttgtaatcga aaaaatcgca tacggcggtc
     1921 aagcaataaa aacatatgaa atagacaatt atccgggatt tgcaaacaat ccgacaggtg
     1981 aagaactttc aaaagcaatc gaagaacacg caagaaaatt cggcgcaaca tttgtttcgg
     2041 aaaatgttaa gtcaatcgaa aatgccgaat acggcataaa ggttattcac acaagaagaa
     2101 atacttatat ggtaaagtca ataatttttg caacaggtgc agtaccaaaa acgctcggtg
     2161 cagacggtga agaagatttt accggtgcag gtgtttcgta ttgtgcgact tgtgacggcg
     2221 cgttttttaa ggataaggac gtgtgtgttg taggcggtgg aaatacggct atggaggacg
     2281 cattgtatct tgcgggattt tgccgtaaag tctatgtcct aaacagaagc aagaagttca
     2341 gagcggcggc tacaatggtt gaaaacgtaa aaagaaacgg caaaataacc gtgcttacag
     2401 ataccgtagt tgataaattc gtaggctcaa atatgcttga gggtgttgat attagaa
//
LOCUS       DAWACS010000197         2450 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4454_length_2450_cov_1.445094, whole genome shotgun sequence.
ACCESSION   DAWACS010000197 DAWACS010000000
VERSION     DAWACS010000197.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4454_length_2450_cov_1.445094"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1721..1740
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          618 a          587 c          367 g          858 t
ORIGIN      
        1 tcccaccgac ttaacgtcac tttttgaggt caaaatttct ttcagtgttt tctgttcttc
       61 acattcgtac ggcacaaaga acaaatcaaa ttcttttgat ttttcaatca cttcattgac
      121 agtcataaat tcagacactg tcggaacaat acccctgccc gactgtttcg ccgccgattc
      181 ggcaatcttt tgccaacggt caatcttctt tttctcgtcc tttttattgt ctattttcac
      241 aacacatctt gaaagtttca caggcacaat ttcagatatg cccagctccg ttgttttctg
      301 aatgatgtac tccattttgg acgcttttgg aagtgcctga aaaagtgtaa cctttatatt
      361 cggctctgtt tcgctctttt gtcttgatac gatattcaaa agaattttct tcggctctat
      421 cgtatcgatt tgagcgtcat aatccgttcc ctgtccgtca cacaccgtaa cgttatcgcc
      481 tttttcaaga cgaagtaccc ttgaaatatg cgttacgtcc tcgctgtcta ttataatttg
      541 attatctgat attttatctt gtgttacaaa aaatttaggc attttcaaaa atcctttcaa
      601 aatttctatg acttattttc tctgcttgtt cttcagtata accccttgca agcagtctgt
      661 caaatatttt atacatatct tcaacaccgc ttataccttc gggcagacag tcaacaccgt
      721 caaaatccgc accgataccg atattatcct caccgccaag ctccataaaa tgctcaatat
      781 gctttacaat atcgtcaata ccgcatttat ctctgtttgt taaaaacggc ggataaaaat
      841 tcactccgac gcaaccgccg ctgtttttaa taattttaaa ttgttcgtct gttaaatttc
      901 ttttatgggg acatataaag tccgaacaag aatgtgttgc gacaatcggc atttttgtta
      961 cctttgcaat atcgtaaaat gactttctgt taaggtgtga aacgtctata agcataccga
     1021 ttttattcat ttccgccaca accgtttttc cgaaatcggt cagtcctctg ttttcgtctc
     1081 tttccaacgc acccgcagca atatggtttg aatagttcca tgttagtgcc gcgattttta
     1141 cgccgagcct gtgataattg cgaagtgccg aaagtgaata tatcccctcg ccgccctcaa
     1201 tgcttaaaac agctttaaca ttctgcggta gattttgcgt gtgcgtgtgg aatgtatctg
     1261 ttaaattcat tgttcgctcc gcaccgcacg tcttatatat cggatctata aagcaagcaa
     1321 aaacttgcgt atagctttta tattccgaca ttctttttaa atcaacatga cagtcatttt
     1381 cattaaggct tttaccgtca tcacgcacgc aacacaatgt atcgcaatgt gtgtcaaata
     1441 cggaatagtt atttttagtg tccctattca ttttagtgtc cccttattaa aaagcatttt
     1501 caatatgatt aactttaaca agaaataatt ccccgttttt ctcttcataa aatacttcta
     1561 tcacatcaaa tcgcatatcg gtattttcca catctgcata aaccatcgcg gcttttttca
     1621 cccgttcctg ctttttgcgg tcaacatatt cgctcggctc accgaataaa ttactctttc
     1681 tcgtctttac ttccacaaaa acaagtccgc cgtcctttgc nnnnnnnnnn nnnnnnnnnn
     1741 aatttcgccg aatttcaaac ggtaatttct ttcaagtatt aagtaatcca tttcaacaag
     1801 ataatcctct gccgcctttt ctccgaaatc tcctatttcc tttgtcgtca taatcattct
     1861 cccaataatt ttcttaaaaa tgttcttctg tgtatcggac acggaccgta ttttaaaata
     1921 gcctcattgt gtgcctttgt gccgtaaccc ttatgctttt caaagccgta ctcgggatac
     1981 ttttccgcca tttgacatat aaatctgtca cggctcactt ttgccaaaat agacgccgcc
     2041 gctattgaaa tactcttact gtcacccttt acaaccgttt cgtgcggtat ttccattccg
     2101 cttattctgt taccgtctat aagcacaaaa tcgggtgcag tgcttagtcc gtttaccgca
     2161 ccgttcattg cctcgtacgt tgcgtttaaa atatttattt cgtcaattcg tttttcgtcc
     2221 acactgaata tattatatgc gattgccttt tcggttattt cttcaaacaa ttcttctctc
     2281 tttttttcgg aaagtttttt tgaatcattt attcctttga ttataatgtc attcggaagt
     2341 ataaccgccg ccgcatatac agggccggca agcggacctc ttcctgcctc gtcaactccc
     2401 gcaacaaccg tatatccgtt atcgcggcaa ttatgttcat actcatacat
//
LOCUS       DAWACS010000198         2448 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4460_length_2448_cov_2.325533, whole genome shotgun sequence.
ACCESSION   DAWACS010000198 DAWACS010000000
VERSION     DAWACS010000198.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4460_length_2448_cov_2.325533"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    499..508
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          737 a          479 c          445 g          777 t
ORIGIN      
        1 accttatcct gtggcaacaa ctgcgaataa actttatcaa taccaatctg ctctgcggta
       61 taatcggcgt ttctctttac gtcgcctgtg agcataacag atttgatatt aagctgtttc
      121 aatgctgaaa ttgcagcttt actgtcagct ttgatttcat cagatataac gatatatccg
      181 gcaaattttc cgtcaactgc aatgtatata atacttccga tgtgtgattt tgctttttcg
      241 tgagagatat tgttgctctc aagaagtctg ccgttaccgg ctaaaacttt atgaccgtct
      301 atatctgcac tgatacccat accggaaatt tctgtgtagt ttgagatttt tgcagtgtca
      361 acgatgtctg tgtaagcatt tttaaccgat actgcgatag ggtgtgttga gtaaaattca
      421 gaataagcgg catattttat aagttcgtct ttgccgataa cagaatatac gtcttttacg
      481 gcaaatacac cttttgtann nnnnnnnntc tgcaactaca ggttttgcct ttgtaagggt
      541 acctgttttg tcaaatacga cagtatcaag tttagcaagc ttttcgataa agttactgcc
      601 cttaacaaga ataccgttct ttgacgcaca accgataccg gcaaagaaac caagcggaat
      661 tgatacaacc aatgcacaag ggcaagaaac cacaaggaac ataagtccgc gatatatcca
      721 agttttgaaa tctccgagaa acaggggagg aacgaacata actattaacg caagtgcaac
      781 gacaatcggt gtatagtatt tagcaaatac ggaaataaat ttttccgatt ttgctttttt
      841 gctttgagcg tgttcaacca tatcaagaat ttttgaaacg gtactttcgc cgtatgtttt
      901 ttctacttta atatatatag gtgagcctgt attcaaactt ccgctcaata cagtatcacc
      961 cgtaacagcc ttacgcggta cgctttcgcc tgtaagtgct tttgtatcaa gatatgtttc
     1021 gcccgaagtt attgtaccgt caagaggaat tttttcgccg ggttttacaa ttataatatc
     1081 tccgattgaa atttcagacg gagaaacttt tgtaatcgtg ccgtttactt ctgtattggc
     1141 atagtcggga cgaatatcca taagttccgt aattgaatta cggctctttt gtgccgcacg
     1201 ttcctgcaat gcctcgccga cttgatagaa cagcattacg gcaactgcct cagggtgttc
     1261 gccgactgca aatgcaccga tacttgctat actcattaag aagttttcgt tgaataagtg
     1321 tcctttgaat atgtttttaa tcgcattcaa aactatatca tagccaagaa caatgtaagc
     1381 gactataaat aaaatatctg ataccaatgg ggaagaaagt acgttttctg ttacgatacc
     1441 tgccgcaaaa agtattgcac ctgctataag tcttatgata agaacttttt cattttcctc
     1501 gctatgttcg caaccgcaac cacatccgca gccacagcca ttttcgtgat gattgtgatc
     1561 gtgaccgcaa ccgcaatggt cttcatgctc gtgttcgtga tgatggtcgt gaccacaacc
     1621 gcagtggtca tcatgctcat gatgatgttc atgtccgcag ccacaatgag cctcgtgatc
     1681 gtgaccgcaa ctgcaatgat ccttatgttt atgatgatgt tcatgttttt cacaacagac
     1741 atctttattt ttttctgaaa tattactatc gttattcttt ttcatatcta cacctccata
     1801 cttgaatata tgaataactg ttcatatatt cattatacgg tatgctctat catttgtcaa
     1861 tagttttatt caaaaaataa aaataatact taaaaatatg ttttttcacc gcaaaagagc
     1921 ggtgcttccg aaaaaacacc gctgtttgcc actaatattt tgaaagtgga gttaatgtaa
     1981 gattagttgt tgcacataca agtaacgata acgataataa taagaaccca aataatcata
     2041 tcaagatcaa aatcgcaacc gcctctttga caaccgtttc cacaacattc gcccattgtg
     2101 agtgcctcct ttcaatgttt tgtaatatat attatgacta atctattaat tttgcacatt
     2161 gtcccatatt ttacttgaat ttttcttcaa aatgggttag aatagtagta gtgatatttt
     2221 cttggggagg taaatctttt ggataagaag aaaattgaca gaataaatga acttgcgaaa
     2281 aaggcaagaa gttccgatgg gttgacgcct gaggaaatga cggagcgtgc aaagctccgt
     2341 gaagaatatc ttaatgccat acgtcagaat ttcaaacaga cgcttgacaa tattgaaata
     2401 attgataaag gagaataaaa aatgtcacta ataattcccg aaaactat
//
LOCUS       DAWACS010000199         2447 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4462_length_2447_cov_2.160535, whole genome shotgun sequence.
ACCESSION   DAWACS010000199 DAWACS010000000
VERSION     DAWACS010000199.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4462_length_2447_cov_2.160535"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1265..1274
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1726..1735
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          831 a          392 c          548 g          656 t
ORIGIN      
        1 caatatgtat aaattatacc caaaagcatt ataataagta tagtacggag gtgtgtacag
       61 tgataaatga tacagataca gaaagtaata tagcggaaca ggaacgacgg aatattgagg
      121 atttgggaag tgttgaaaca aaaaatccga gaggtaatat acattgtctg aatataatcg
      181 gtcaggttga ggggcatatg gtattgccgc cgcagaacaa gacaacgaaa tacgaacacg
      241 ttctgccgca gcttatagcg attgaggaaa atccggaaat tgacggactt atggtgcttt
      301 taaatacggt aggcggtgac gttgaggcgg gattggcgat agctgaaatg atagccggaa
      361 tgagtaaacc caccgtttcg cttgttttgg gcggtggaca cagtataggt gtaccgcttg
      421 cggttgcgac agactattca ttcattgcac cgaccgcaac tatgacagtg catccactca
      481 gaatgaacgg cgttgtaatc ggcgttatgc agacatttca atattttcag aatatgcagg
      541 acagaataat tgattttgtt gtttccaact caaatatatc atacaacaaa tttaaacagt
      601 taatgctcac gactgatgaa atagcaaacg acgtggggag tatattattt gcaaaaaagg
      661 cggttgaata tgggcttata aatgaaatgg gcggacttaa agacgcactt gaaaaaatgt
      721 atgagctgat agaaaaggag agagaaaata aaaaatgatt tattcaatag tgccgacaga
      781 tgttgtgttt ttcgacaata ctcaaataag acaaagacat ctaaaaatgt ataacaacgt
      841 gacacttgaa ttgacagacg gacgtgtgga gagaattata agcacaaatc ctcaggatta
      901 tctgaaatat cataatttac tcggttcaaa caatatttag aaaaaatatc ttgcaaaact
      961 ttgtcttatg gtgtataatg taacagattg ccgtactcgt ggcaatacac taatgaagga
     1021 ggaaaagaat atgcaagata atgtatatga cgttttgctg gagcgtggac ttattgccca
     1081 gacaacacac gaacaagaga tacgagaatt attaggaaag gaaaaagtta cgttctatat
     1141 tggctttgac cctacagcag acagccttca tgtaggacac tttttgcaga tggtcgtaat
     1201 gcgacatatg cagaattacg gacacagacc gattgccctt gtcggcggcg gtacaggtca
     1261 tatcnnnnnn nnnntgacca taactgtgaa tgtttcaaac agcagttatc aagagttatt
     1321 gatttctcgg acgataaagc tataatggta aacaatgccg attggctttt agaccttaat
     1381 tatgtagaat ttttgcgtga catcggttca tgtttctcag taaataaaat gcttactgcc
     1441 gaatgcttta agcaaagact tgaaaaggga ctttcattcc ttgaatttaa ctatatgctt
     1501 atgcagagct atgacttctt aatgcttaac cgtaagtatg actgtaaaat cgaattgggc
     1561 ggcgacgacc agtggagtaa cattcttggc ggtatcgacc tttgcagacg taaggaccaa
     1621 aagcaggttt acggtatgac atttactttg cttacaaaca gtgaaggtaa gaaaatgggt
     1681 aagacacaat cgggtgctgt ttggcttgac cctaaaaaga ctactnnnnn nnnnnaggta
     1741 aggaacttaa cgaagctaag agaattcttg cttatgaagt aacaaagctc gtacacggtg
     1801 aagaagaggc tgaaaaagct aagaaaacag cagaagaagt attcgctaaa ggcggagtaa
     1861 gtgcagatat gccgacaagc gttatacctg acgttgtcgg aatgggtgta cttgatatgc
     1921 ttgttaagac aggattgttg ccgtcgaagg gtgaggcaag acgtcttgtt caacaaaacg
     1981 gtctttcaat caatgatgta aaatacaatg acgtaaacgg tgttgttacg gaagatatga
     2041 ttacagatga aggtatgatt atcaaaaagg gtaaaaagac attccacaga gttgttaccc
     2101 aataagacaa aaattaaaag cgttcacatt ttgaagtgaa cgcttatttt tttagaattt
     2161 tataattccg agtgcttcaa gtattgaaga aatcaatatt gcaacgataa acacaggtgc
     2221 aacgtattta atcataatat cgaaaagttt ttgacgtttg aatgctgatg atatgcgtac
     2281 ttcatcgtgt attgagttga cgccgataac gtatgcgaca aatatacagg taagcaatgc
     2341 gcatataggc ataagtattg aatttgtggc aaagtccatc atatcaagaa ttgtcatacc
     2401 gcctaaagtc gctttatccc agacattgta tcctaaaatc ggcggta
//
LOCUS       DAWACS010000200         2447 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4463_length_2447_cov_2.135033, whole genome shotgun sequence.
ACCESSION   DAWACS010000200 DAWACS010000000
VERSION     DAWACS010000200.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4463_length_2447_cov_2.135033"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    271..280
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          637 a          597 c          381 g          822 t
ORIGIN      
        1 tacaagcagt ttttgtgtac cgaacacttc gtgacaacag ccgaacgcct taatttcagg
       61 gaacacctta tacaaagtct ttacgcacat tgtcataggg tttgtgtagt tgattaccca
      121 tgcgtcaggg caatattttt ttatattctc tgcgatttct tcaaacatcg gaattgttct
      181 taacgctctc aaaattccgc ccggccctgc cgtatcgccg actgattgat atatattgta
      241 tttttccggt gtatgtacat cgctttccat nnnnnnnnnn gaatgtaccc ggtaaaatgg
      301 atataacaac aaaatccgca ccctcaagag cctctttttc agtttcaacg gctttgtact
      361 tccactgtga cttacagcct tctatatcct ttatgccgtt gcctatcttt tcgtttttct
      421 ttgccgcctc taaatctata tcgtaaagat aaacagtacc gcacatatcc tcacacaccg
      481 caaggtcgct cataaatccc cacgcccaac cgcgtgagcc gccgccgata taagcgattt
      541 ttatatcttc gtatttattg ttaccgtatt tcatttttct gtcctccgct ttaattttat
      601 gacttaatta taccactgat tttcaactta aacaacaaca atattgctcc aaatcaaaaa
      661 tgtactgcct aaacagtaca ttttttgctt aattattttt caaatattcg tctacaccct
      721 cagcgatagc cttaccgatt tcatcacttg acgactgcaa tatcgacaag tctgagctgt
      781 cgtaaaaacc tatttccata taagcaatcg gaacagggca tttattgaac aatatcaaat
      841 aaggtgtatt tataggcgaa ttttgcgaaa gtcccgttgc cgccgcaact ttttgattta
      901 tgatattacc catttcattg gacttttcga caaagtcact gcctataaat ttctgttcgt
      961 aatccgtacc caaagctatg tatgaagttc cacgaccgcc tcctgcattt gagtggatac
     1021 aaacatatgc cgcagcatcg ggatttgacg ttgtatcatt attagggaaa caatacgata
     1081 cacgcttgtc cattgacgga ttttgttcgt ttgacgttct cgtcatacga acttcataac
     1141 ccatttgttc aagatacttt tgtgtcgcca aggcattgtc aagattgatt tgcggttctg
     1201 tatctctgtc ggcattgccc attgaatacc aacacgacgc gttttcaggc gacaagcctg
     1261 tacaaccgct accgtgacaa ttttcgccga atgtaccgtt tttatagtgt cgccattcgc
     1321 cccaattacc ttttgactcg ttatattcat aaccttcggc tgttttttcg tcagatgtca
     1381 tttccgacga tgacttaccg tgtccggcgt caagtacaat taccttttta cctgctccgg
     1441 attgactttt acccgctgta acatcatcta tccctaacgc atcagccgcc gtctttactg
     1501 cattactttc acctgttgca ccgtcaccgt cacttttaac ttcttctgtc gactgcggtg
     1561 caaacgtagg catttacatt tccgcaagtt tggtttcggt atcaagacgt tgcttttcca
     1621 tattactttt ttgtatattc actccgcata ttacagctat acaaaaaact atcgacaccg
     1681 ccaaaaacac tgccgcccct cttaaacaga ctttaagtct tttatcactc ataatcatcc
     1741 ctgcgtcctt tctttgtaag acgtttatct ctgtacattt ccgcgtcggc tcttgcgaca
     1801 agattttcaa aagtcgtatc accctccgta tacaacgata caccctttgc aaaatccaaa
     1861 ttcacattat tttcttcatt gtattttact ctgcacttct caatgcgttt aagcactttt
     1921 tctatgccgt catcggtaat gtcgtgaatc aacactacaa attcatcgcc gccgattctg
     1981 tatatcttac cttcatttcc gactgcctca cgaagtactg tcgcaatacc tcttataaga
     2041 acatctccgg cactgtgtcc cattgtatcg ttagcacgtt taagattgtt taaatcaata
     2101 gatatacaag ccggtctgtt gttgccgata cattcgttta tatcgttttc aaaagacagt
     2161 ctgttgttaa gctcggtaag tccgtccgta aatgcaagat gttcgtaaac ctccgcttta
     2221 aggtgacttc tgctttttgc catatactgt cgtatcgtcc gtattacctg cattaataca
     2281 aatatataca gccccattga gaagaaatat atcgttttgt catcgctgat ccgatacaat
     2341 ctcactatat caataatact tcctacaatc gggaacataa ttgatatcaa catatattgt
     2401 atactcttct ttttgtaaaa aataatgatt gttgataaaa tcaatat
//
LOCUS       DAWACS010000201         2446 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4470_length_2446_cov_1.675031, whole genome shotgun sequence.
ACCESSION   DAWACS010000201 DAWACS010000000
VERSION     DAWACS010000201.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4470_length_2446_cov_1.675031"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          635 a          570 c          367 g          874 t
ORIGIN      
        1 tcgcataagc cccgattcaa caacaggtct gccgtcatct gatattgcaa ccgcaccggc
       61 atttttaagt tcgccgattt cagcaagctc cttaccctca agaccttttg aaattgagcc
      121 tatcggatat acgtttacat gaccgacttc cttagccttt gtctttatat aagtaacaat
      181 cggctcactg tctgttacag gctttgtatt cggcatacaa gcgattcatg tgataccgcc
      241 cattgcggca cttcttgtac ccgattcaat atcttcttta tattcctgtc ccggatctct
      301 caaatggctg tgcatatcaa caagtcccgg tgacacaatt ttaccttctg catctataac
      361 ggtaacgtca ccgtcggcat taatgttttc gcctatttcg gatataacgc cgttatccac
      421 aagtacgtcc gctactttat caaaatcatt tttagggtca actaccctac cgcctttaat
      481 taatatcttc atattctttc cctttcgtta gtcttcaaat ccgcctcttg acaatatata
      541 cataaccgcc attctgactg caacaccgtt tgtaacctgc tcatttataa cgctttgttc
      601 gccgtcaatg acgcttgatg aaaattccac gccgcgattt accggtccgg ggtgcattac
      661 aagtgcgccc ggtttggcaa gttttaatct ctcgttgtca actccgaaga atctgtaata
      721 ttctcttata cttgggaata agcctttttt ctgtctttca agctgtattc taagtcccat
      781 aacaacatct gcgtctttta tcgcatcctc aacattgtta aatactttta ctccaagtct
      841 ttccattccc ggaggcataa gcgttgtagg gccacctacg cttacatctg caccaagctt
      901 tgtaagtccg taaacattac ttctcacaac tctgctgtgg taaatatctc ctataatcgc
      961 aactttaaga cctttaaaac tgcccttttt ctccttgatt gtaagcatat ccaaaagtgc
     1021 ttgcgtaggg tgttcgtcca taccgtcacc ggcattgatt accgacgcac ttacaagcgg
     1081 agcgataaga tgcggtgcac cgctcatatt atgtcgcatt acaagaatgt cagtacccat
     1141 tgcattgatt gtttcggcag tgtctataag cgtttcacct ttctgtaccg atgaaccgct
     1201 tgcagtaata ttagccgcgt tggcactcat atatttagcc gcaagctcaa aagataatct
     1261 tgttcttgta ctgttttcat aaaacagatt tacaacagtt ttgccttgaa gatgaggagt
     1321 ctttttatta gtctgaccta atataagttt catcgttccg gctgtttcaa gtatgttttc
     1381 tatttcgcac ttatgtcttt aagtccgagt aaatctttct tcattattaa ttatccccct
     1441 taccgcatat tgatactttt tctaatttat catcgtttat tacaacatca atatattcat
     1501 catgtgaagt cggaacatct tttccaatgt agtcagcacg gataggaagt tctcggtttc
     1561 ctctgtctac aagtaccgca agttcgattt tttccggacg tcccatgtca aaaagttctt
     1621 ccatcgccgc tcttatagtt ctgcctgaat acaatacatc gtcaactaag attattttct
     1681 tatcctctat tgaaaatgca atgtcgtttc ccgcaactac cgggtgcttg ctgagtcttg
     1741 tcaaatcatc tctgtaaaac gttatatcaa gacttccgag ttcaagctta ataccctcga
     1801 tttcttcgat tttcttgatt ataagctttg caagtgatac aatacctatt atgacaagat
     1861 tttcagttcc tcgatttctc tcaacaattt catatgcaat tcggctgacc gctctttttg
     1921 aggtagcctc atccataagc actgtttttt cttcgtaatt cgccataacc tttcctccgt
     1981 cttggctgta ctgtattata ttacgccata tttatttata ttattatata acataattca
     2041 ctttttgtct atacaatgac agacaaaaca ttcaaaataa tttacctatg ttctttcttg
     2101 ggtaagaaaa aagcacgttt aaaacgtgct tttgtttttg tttatcagac aagaatagaa
     2161 atcacatcat tttgtgtcgg tcttgtcgga agtattattc ttttaatttt ataaatttta
     2221 tcttttacta agatattttc accgactttt aaagtttcac aactttttac aagtagaatt
     2281 tttccaaaag atgtttcata ttctgctatc aattccaact ctttcataat cctcaccatt
     2341 ttacattgca tatatatcaa tattttgcca caaatccttt atcggtactc cgtctatctt
     2401 atgattatcc aataaatcct gtgtatcact ataataattc ggttct
//
LOCUS       DAWACS010000202         2445 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4471_length_2445_cov_4.157741, whole genome shotgun sequence.
ACCESSION   DAWACS010000202 DAWACS010000000
VERSION     DAWACS010000202.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4471_length_2445_cov_4.157741"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          713 a          555 c          481 g          696 t
ORIGIN      
        1 caccaaagtc gtcttgcccg aacccgacac tcctgtaaca acggtaagtc tgcctttggg
       61 aatgtcaact tccaacggct tgaccgtatg tatttgagat gtggacaaat gaatatttcc
      121 gtttacgaac atttcttcct ttgcggcaca tgaccttagt tttgtttcgg ctttaccgga
      181 tataaaaggt cctatttgag aagcggggtt tgcttccaca tctgcgattg taccttgtgc
      241 aatcacatga ccgccatttg cccccgcttc cggtcccatt tccacaatcc agtctgcctc
      301 tttcaaaatc tgagtgtcat ggtctaccaa aataaccgaa ttgccgtccg atacaagatc
      361 gtgcataaca cctgtaagtc cgacaatatt agatgggtgc aaaccgattg acggctcatc
      421 caacacatag agaacgcctg tggtacggtt acgcacagca cgggcaagct gcatacgctg
      481 acgttcgcct gtcgaaagtg tggctgccga acggtcaaga gtcagatagc caagacctaa
      541 atcaattaac ctcttcgcca tactttcaaa agtttcacag atactttcag ccataggttt
      601 catctcatca ggcaggcttg tcggaacacc acgcacccaa tccacaagtt cggaaagtgt
      661 catagtgcaa acttcatcaa gcgatattcc tcttaatttc ggtgcacggg cggcggcgga
      721 aagacgtgtt ccgtgacatt cgggacaaat atcctctttt aaaaatttct ctactcgctt
      781 catgcctttt tcatctttga cttttgcaag agcattctcc accgtgtaaa cggcattaaa
      841 atatgtaaaa tccaattcac ctgcattttc cgtatttttg gatttgtaaa aaaggtgttt
      901 tttaaccgcc ggaccgtgat agacaatatc tttttcctgt tccgtcaagt cacggaacgg
      961 tacatctgta cgcaccccca tctcacggca aacatccgtc attaatgacc acatcaagct
     1021 gttccacgga gcaactgcac catcatcaat cgtaagagag tcattaggca caagtgatgc
     1081 catatcaacg gtgtgaacaa ttcctgtacc accacatttt tgacaagcac cttgcgaatt
     1141 gaatgccaac tcctctgccg acggtgcata aaagtgaacg ccacattccg agcagactaa
     1201 ttccttccct gcggcaaccg ccagtgacgg ttttaagtaa tgtccgtttt ggcaacggtg
     1261 gtttgataaa cgtgaataca taagtcgcaa actgtttagc aactccgttc ccgttccgaa
     1321 tgtactgcgt attcctggta taccgggacg ctgatgtaat gcaagtgcgg cgggtacata
     1381 aagcacctca tctacacttg ctttcgatgc ctgtgtcatt cggcggcgag tgtaagtaga
     1441 cagcgcttcc aaatagcgcc gagaaccctc tgcatataat acacccattg caagagagga
     1501 cttacccgag cctgacactc ctgcaatacc tacaattttc cccaacggaa tatctacatc
     1561 tatattttta agattgtgga cttttgctcc acgaataagt attttttcta tcattggttt
     1621 tactcctaaa tatcttttcc aaaaacgcac tgaaaaaagc atatcacaag catatctatt
     1681 ttgtcaatat tctgccaaaa atcggaaaaa agaaaacaaa gtaagcgaat gggatatata
     1741 ggtattttta tatattgcgt ttgtgttgtc gatatgctat aatattttca aaagaggtga
     1801 gattcatgga ctttttaata atgaatagag ataccgttgt ggcagaatgg atagataaca
     1861 aattaaatct gattaagccg tcacttgctc caatgtattt ggaacttaca agcaatgttc
     1921 cgaagtggct tgaaacaaga gctatcgact cacacagagc aaattcaaga cttcttaaaa
     1981 aggctttgcg tcttaccgaa cgtgacgata taggctctgt tttatcagtg aatgcagtca
     2041 ccattacgga taattattgg ataaaaccga ttaacagcga cctttgctat gccgatgttc
     2101 gttttgacaa cgactatttt gccacacttg ctttaaccgg cagttacgac agttttaatc
     2161 gagctgtgca tagcaaaagt acaaaaactc ccgaacttac caatataggt agctttgaaa
     2221 agtgttggaa actgattaac ggcgaatggt ggatgtacaa aaaggctaat cacgatgaaa
     2281 cgttttcaga atttttcata tatcaactcg gaatggaatt agactttaat atggctgaat
     2341 ataaacgtgg taatggtgtt attaaaacaa aggattttac cgataatgcc atggtcaatt
     2401 ttgagcctgc atttaatttt atgaatgaat gcgaggacta tatac
//
LOCUS       DAWACS010000203         2431 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4497_length_2431_cov_1.845118, whole genome shotgun sequence.
ACCESSION   DAWACS010000203 DAWACS010000000
VERSION     DAWACS010000203.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4497_length_2431_cov_1.845118"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    325..334
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          602 a          527 c          463 g          829 t
ORIGIN      
        1 gttgtgacgc tgtcaagagt cttgtcagcc ttgtaataag cgtgtacaag agttgctttg
       61 tttacatccg gcgcaatatt ttcaaattta taaacactgc ttgtttcgcc tgattttgcc
      121 acagtcactt tgcctgttaa agccttgtgc tttgcaagtt ttaatgtata atcatatgta
      181 cccggaaggt aatatgcctc tactttaccg cttgcaggaa cagtatatgt atcatcgccg
      241 attgtaagaa ttgctccttc aaccgattca ccgtctttgt ttgattttac attaaatgta
      301 accttttctc tgtcaactgt ttttnnnnnn nnnnatcaat aaagaaaggt ctgtcccaat
      361 cttttttctg atcatattca atagcaattt cgccgatatt actgcctgag gcaaattcag
      421 ctgttgtatt acccaatgta acttctccgc cattttcatt aaatgtcaca tatgcgctga
      481 tgtctgtata tttactgtct gagaatgccg aatagcttgc atctttaact tcatcaccca
      541 ctacaagtgc tattcttctt gcccaatcac tgccaccgct tgtgactctg aatttgaaca
      601 attctgtgcc gtcagttgac ttaaatacca tgtcccaagt tgctgtattt tgcataaagc
      661 tcttaaccga acctgcttta aatgtcattt taacatcttt tgttttacta cccattccgt
      721 tcggaacttt aagaatgctc tttccgtcat tagcagtaag agatagtgct ttgtttgtac
      781 ctccccagtc atttccgata actacatccc atttgtaacc gttaccgtca acagcacttt
      841 caccgtcaaa attttcgctg atgtccacac tgccgtccca tgacgggtca actgtaggag
      901 cagctgtagg cgcctctgta ggcgctgttg tagcctctgt tgcttctgct gttgcagtcg
      961 gtgctgttgt tgcttctgtt gcttccgctg ttgcagtcgg tgctgttgta gcctctgttg
     1021 cttccgctgt tgcagtcggt gctgttgttg cttctgtcgc ttccgctgtt gcagtaggtg
     1081 ctgttgttgc aacagttgaa tctcccgcat tttctgcgtc aaacatagtt attctgttgt
     1141 caaaaacatc gaacaaactg tcttttgaat ttacaggagc cgcaaagctc gtaaatgcag
     1201 aagctgttat tgccaaagct gtaatgattg acaatagttt ctttttgtta ttcatgtaat
     1261 ctctccctct ctttattaat tttatacggc acaccaccgt acaatataac atgatagcta
     1321 tattatatac tatttactat tttttttcaa tagtatttct acgactaaaa tattatcact
     1381 gatttttaaa cattatttgt tacctatatt tgtataagat attgatttta tatcgttttt
     1441 cccttataat aataagtaat aaaacaattc ggagtgataa aatattttga gtaaagcaac
     1501 gcaattttta aaaggtgcaa ggctcggtat gcctattgca ctcggataca tacccgtttc
     1561 attttcattc ggtatgcttg ccgtacaaaa cggaataccc gcttgggcgg caatagtaat
     1621 ttcaatgact aaccttactt ctgccggaca atttgccggt atgaacataa tttccgccgc
     1681 cggcggtatg tgggaaatgg caattacgac atttgtgata aatcttcgat atatgcttat
     1741 gtcgctttcc ctgtcacaaa aaatcggtaa aatgtcgctt gcaagaaggc ttatcacagc
     1801 attcggcata acagacgaag tattcgccgt tgcgtcaaca acaccgattg ccattacatt
     1861 tccgtttatg ctcggactga ttgcaatacc gtacatcggc tggacactcg gcacagtgct
     1921 cggtgctttt ataaacaatc tgctgccgac atcacttgcg gacgcaatgg gaataacatt
     1981 atacgcaatg tttatcgcaa taattatccc tgcgtcaagg agagataaaa acgtactttt
     2041 tgtacttatc gccgcagtta tattgagttg cgttctgtat ttcgcacttc cttttatatc
     2101 atcgggctgg gctgttatca taacggcagt gttggcgtcg gcattcggtg cgacgttctt
     2161 tccgcacaat gaggagggcg aaaaatgaaa atgattgctt taatggcagt tatggcaata
     2221 gtgacgtaca taattcgtgc gttgcctata acgcttttca gaaaagaaat aaaatcaaaa
     2281 tggcttaaat cgtttttata ttacataccg tatgccgtac tcggcgctat gacgtttccg
     2341 gctatatttt tctcgacagg ctcggttttc tccgcctcgc tcggattggt cggtgctatg
     2401 cttatggcct ttttcgacaa ggggcttatg g
//
LOCUS       DAWACS010000204         2423 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4510_length_2423_cov_2.500000, whole genome shotgun sequence.
ACCESSION   DAWACS010000204 DAWACS010000000
VERSION     DAWACS010000204.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4510_length_2423_cov_2.500000"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          727 a          452 c          630 g          614 t
ORIGIN      
        1 gagcatagtc cgagcatgaa attccacgcg cgcgaattca aggcggtgag cggaaacgac
       61 tacagtatta cagcgagatt tgagtcgcag gatggagaaa agatattcgg catgggtcag
      121 tatcagcagc ccgagcttga tttaaagggc tgcgtgctgg aattggcgca gcggaattca
      181 caaatcagcg tgccgtttta tctttcaaac aaaggctacg gatttttatg gaataatgcc
      241 ggaataggcg aggtaatgtt cggaaaaaat tacactcaat ggcgcagcaa gctgtcagaa
      301 gaacttgatt actggataac cgctgacgat accccaaaag ctattatgga aaactacacg
      361 gaggttgtcg gacgcgcgcc ggaatttccc gaaaacgcgc tcggcttatg gcagtgcaag
      421 ctgcgctatc ggacgcagga cgaggttctg gaggttgcgc gcgagtataa aaggcgcggt
      481 attccgcttg atgttatagt tatagacttc ttccactgga tacgacaggg cgactggagc
      541 tttgaccctg aatactggcc aaatcctcag gcaatgatgg acgagcttag ggaaatgggc
      601 ataagatgca tggtttcaat atggcctacg gtggacagaa agagcgtgaa tttcaatgag
      661 atgtttgata gaggactatt cataagaacg gagcgcggtt cgatacaatg ctttgatttt
      721 cagggtgaca ctgtgattta tgatgctctt aatcccgagg cgcgggaatt tatatggaac
      781 aaggcggaag aaaattatta taaatacgga atagatatgt tctggctcga cgaggcagag
      841 ccggaatatt cagcgtatga ttatgagcat taccgtcatt atacagatcc gtctttaaag
      901 gtcggcaaca gctatccaaa ggctcatgca aaggcatttt atgacggaat gaagactcag
      961 gggcagacgg atatagtaaa tctgcttcgc tgcgcatggg taggcagtca gaaatatgcc
     1021 gcactcgtat ggtcgggcga tattaaatca aactttacgg ctcttagaga ccagctaagc
     1081 gcgggactga acataggaat tgcgggaata ccatggtggg tatcagatac tggcggattt
     1141 ttcggaaacg tccttgacga gcattttaac gagctgctta tacgctggtt tcagttttca
     1201 acattctgtc ctgtactgcg tatgcacggc gacagaggtc cgcatgatat tccacgctta
     1261 tccgatctcg attacggcgg cggtttttct gaaaccggca gacctaatga gctgtggagc
     1321 tacggcgagg atgtttatga aatactgaaa aagtaccttg atatacgtct tggtatgaaa
     1381 gattacatta agtcggttat ggatgaagca agcgaaaacg gctcgcccgt aatacgcgca
     1441 atgttctatg aattcccgga ggacggaatg tgctgggata tagatgacca gtatatgttc
     1501 ggctcaaaat atcttgttgc gccggtaatg tatcagggca tgacagaacg gaaagtatat
     1561 ctgccgcacg gtaattggaa agatattcac acggggaagg tatatgacgg attgcagaca
     1621 atgaatatgc ctgcgccgct tgagattata cccgtttttg aaaaaatata agtattaaaa
     1681 gtctttgtat gcgtaaaagg agggtgtatc atgtcaaaga aattaattac aacaattttg
     1741 acaggagttg tttttatgtc aaatatgcat attgctactt tgccggtaaa agcagcaaac
     1801 gattcggatt tatcggtgcg tttggagaat attgtatatg tgtctccgaa tggaaatgca
     1861 gaaaacgccg atggaaccat cgatgatccg ttttctacag ttgaggctgc cagagattat
     1921 ctgaggacaa aagatattgg caaagatagc aggggaatta tttatttgcg tgaaggtact
     1981 tatcatgtta cgaacagcat ttctttgaca agtgaggatt cttatgttac atatgcggca
     2041 tataaaggag aatcggttga gattacaggt tctgcgacac ttgagaacgg caaattcaaa
     2101 aaattaagcg aggtttcagg agaaaagtat tcctcacaga caagactgcc gtccggagtg
     2161 gctgataagg tatatgtata tgaccttggc gcagcgggga taccagtagg agatgtttat
     2221 aaaaacggat ttaactggac aaaacagccg ccacagcctg agcttattgt aaacggcgaa
     2281 cttcagattc tggcgcgata tccgaataat ggtactataa caaagtcgca tcttgaaatt
     2341 ccaaacaaag gcggcgttcc cagagactac ttcttcgata aaaccgacta ctcaaaaaca
     2401 tatgaagaaa tgctggcaat gaa
//
LOCUS       DAWACS010000205         2422 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4512_length_2422_cov_3.115758, whole genome shotgun sequence.
ACCESSION   DAWACS010000205 DAWACS010000000
VERSION     DAWACS010000205.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4512_length_2422_cov_3.115758"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          771 a          437 c          611 g          603 t
ORIGIN      
        1 cgggggatat tattcatacg tctgccaagc ggaagacggc tgtcatatat aaaacctcga
       61 atcggtacaa ataggttcgg cggtgacagc attacatata tggggataaa ctccgcaaag
      121 aagtgggaca gacttgaaac tttcggcggt aagctgacag agaatatcgt acaagggact
      181 gcaagagatt tgcttgcaaa tgcactgata aatgcggcaa atgcgggata tgatactgtt
      241 tttcacgttc atgatgaaat tatatgtgaa gttccgaacg gttacggttc ggttgacgaa
      301 ctgtgcaagc tgatgtgtat aaaacccgaa tgggcggacg gacttccgct taatgctgac
      361 ggttttgaat gtgagtatta taaaaaggaa taacaaaaat gcaaaagaaa ataaatttaa
      421 aggggtacga ttatgaaaat aagatgtttg gataaaaaag actgctttgc taatgcagac
      481 ggttattgta tttgcttaac gaataatgat ttcggaggaa gaagatgttc gttttataag
      541 acaaagacga aagctgcgac agaacgcaaa aaagttgaga aacaattaaa acgaaaggga
      601 aaaacgggat taatagacat gtataacggg aggggacaat gaaaatatct gtcgggaaca
      661 gccgtacatc aagagcgtgg aagattaaag aattttcgtg ggagaaattt gttcagaagt
      721 gttctcagac aatacgaacg gctgaaacgg tgcaggaata tcgtaaactt cctaaaggtc
      781 agcaggataa tatcaaagat gtgggcggat ttgtcggagg tgaacttcgc agcggtctaa
      841 ggagaaaaga tactgtgatt aaccgatgtc tgctcactct tgatgcggat tacgcagacg
      901 aggatttttg ggagcagatt gaactgttct ttaatttcaa atgcctgata tattctacac
      961 acaagcatac tgcacaaaca ccgagattca ggcttattat ccctctgtca cgtcctgtta
     1021 cggcagatga atatacggcg gttgcacgca gaatagcggc agatataggc atagaacagt
     1081 ttgacgatac aacgtatcag ccgcatagac ttatgtattg gccgtcaaca tcttctgacg
     1141 gtgagtttgt atttcagcac cgtgacggtg aagaaataga tgcggacgca gtgcttgcac
     1201 gttataaaga ctggcatgat acttctgaat atcctgtttc gtcaagacag aaaaaaattg
     1261 tatcacacgc attgaagaaa caggcagacc ctctgacaaa gcagggaata gtaggtgcat
     1321 tttgccgtgc atatacgata caggacgcaa taagcacatt cctttcagat gtatatgaac
     1381 caagcagtct gaacggccgt tatgattata tccccgccga ttccgtagcc ggagtgatag
     1441 tgtataatga caagtttgca tactctcatc acgcaacaga ccctgcctgc ggtaagctgt
     1501 gcaacagctt tgatttggtg cggatacata agttttcata tcttgacaaa gatgaaaccg
     1561 acagtgagaa atcagcctca tttaaagcaa tgacagactt tgcaatgcag gacggcagag
     1621 ttaaagaaca gacactgacg gataaggaaa aggctgtgta tgaggagttt tctgtgattg
     1681 atgacaatga cgatacatcg tggcataagt atttgtctgt aaataagcac ggcgatgtag
     1741 agaacagcat acaaaacctc acgataatac ttcagaacga tccgaaacta aagggaatag
     1801 tttttaacga actgtcggac tgtatagaaa ttaacggtga tgtaccgtgg cagcacccgt
     1861 ctaagtattg gcgggacgct gatgacgcac agctgcttac atatcttaca tacagctacg
     1921 gtaaattcac aagagtgaat tatgatgtgg cacttgcaaa agttgttgat gaccgttcgt
     1981 tccatccgat acggcagtat cttgacggtc tgccgaagtg ggataagcag aaacgtgtag
     2041 atacgctttt aacggattac cttggtgcag aggacaatcc atacactcgt gcggttatcc
     2101 gtaaaacact atgcggagcg gtagcaagag taatggtgcc gggaataaag tttgacacta
     2161 tgcttgtact gtcaggaccg cagggaatag gaaagtcgac gattatatcc aagctgtgcg
     2221 gtgagtggtt taacgattcg cttttattat ccgatacgaa ggataagacg gcggcggaga
     2281 agctacaggg attttggatt ttagagatag gcgagcttgc aggacttaag aaaactgaga
     2341 ttgaaacact gcgcggcttt atatcaaggc agaacgatgt gttccgtgcg tccttcggtc
     2401 gcagagcgac tccgcattta ag
//
LOCUS       DAWACS010000206         2410 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4542_length_2410_cov_2.258599, whole genome shotgun sequence.
ACCESSION   DAWACS010000206 DAWACS010000000
VERSION     DAWACS010000206.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4542_length_2410_cov_2.258599"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          618 a          562 c          404 g          826 t
ORIGIN      
        1 cctgcaagcg cgtggttgac tgaaggcggc atagccgtcc acaaatcctc gcaaatttca
       61 accgcaagaa caaaatcttc acacgcatct gttttaaata atatattcgc accgaacggt
      121 atttcttcac cgtttatatt cacatatgtc acttcatttt taccggccgt aaaatgtctt
      181 acctcataaa attcggcata atttggaata ttaagtttcg gaacaagacc ttttatgtgt
      241 cccccgttta ccactgcggc acaattataa agcgtctgct ccgcaacata cggaagtccg
      301 acaacggcgg ttatatcaag attttccgtt gcttttgcta ttttgattaa gctgtctttt
      361 gcactgtcaa gcaatatttt ctgcaaaaac aaatcaccgc aagtatatcc cgttatgcaa
      421 agttccggaa atactataag ctttatatca ttttgagccg cgtcttcgat tgcctttatt
      481 atatttgatg tattataatc agtgtccgca acctttatat caacagttgc gcttgcacat
      541 cttacaaatc cgtctttcat aaatcaaatt cctttcttta ttctgtccca ataaactttt
      601 gccgactgtt cggttttatt gtccccaaaa tcgtaatatt tagtgtccct aatgttgtta
      661 ggcaaatatt gctgtttgac ataatgattt tcataactgt gcggatattt gtactctgtt
      721 ccgtgaccga gtttttttgc tccgccgtaa tgcgaatctt tcagatgttc cggtatatcg
      781 cccgtatcca taccctcaat atcactcaac gcactgtcaa tcgccattat tgccgaattt
      841 gattttggtg cggttgcaag taaaataacc gcttccgcaa gcggtattct cgcctccggg
      901 aaaccaagct gcagggcact gtcaacacaa gctttcacga ccgatatagc ctggggatat
      961 gcaagtccta tatcctctgc cgaaataacc aaaagtctgc ggcaaatgct cggcaaatca
     1021 cctgccgaaa caagtcttgc aagataatga actgccgcat ccggatctga acctcttatt
     1081 gatttttgaa acgcacttaa aatatcataa tgactgtcac cgtcacggtc gtaacgcata
     1141 gctttttttt gcgttgcctg taccgccgtg tcaagcgata tgtgtatttt tttatctttg
     1201 tttggcggtg ttgcaataaa tgcaacttca agcgaattta aagcctttct cacatctccg
     1261 tttgaaacgt gtgccatatg ccgtattgcg tctttgtcaa cggttacgtc aaagccttta
     1321 taatccgatt gctttaattc ttcaaccgct ctttcaagtg ccttttcaat ctcgctcggc
     1381 tcaatctgtt taaattcaaa cacaacactt ctcgaaagca ccgcattata cacataaaaa
     1441 tacggatttt ccgttgtact tgcgataagc gttatcttgc cgttttccat aaattcaagc
     1501 aaagactgtt gttgtttctt attaaaatgc tgtatttcgt caaggtacag caatactccg
     1561 tccattgcca aaaagctgtc aagcgttgat acaatatttt ttatgtcagc aacggacgca
     1621 gttgtcgcat taagtctatg caaagtttta cccgtttttt gagcgattat gtttgcaatg
     1681 gtagttttac cgactccgct cggaccgtaa aatatcatat tcggtacttc gtcattttcg
     1741 attatgtttc ttaaaatttt tccttcacct ataaggtgct tttgtcctac aacttcatca
     1801 agcgtctttg gacgtattct atcagccaat ggtgttttca ttgtgattct ttacctcttg
     1861 tataagctta ttaacttctt cctccgtaac cgttgtcatt cccgaacgct tacattcttt
     1921 cagcttttcg gcatattctg cggctttatc gtacttgtta agtttcattg caatctgtat
     1981 gccgtgataa aagccctcga caaagttagg tgccgccacg acaagttcat cagatatttt
     2041 ttctgctttt tcatattcgc cttttctgaa ataacaaatt gaaagattat cttttatgtc
     2101 cctgtcatca ctgttgtaat cataagcttc ttcacaaagt tttgttgttt cgtcaaggtc
     2161 atcgtttcta agcaatttaa aatagccaag cataccgtaa atacttgaat ttctgtaacc
     2221 ttcgttcatc attgactgtg catcctcaat cgcctcgtca agtctgccct gcttatagta
     2281 aaccatacat ctttgttgct ttgccaaatt ctttttttcc ggtgcaagcc cttttactct
     2341 tataatcgga tcaagaactt tttccgcctc gtcggcataa cctgtacgta aaagcacgtc
     2401 tgcgtaagac
//
LOCUS       DAWACS010000207         2403 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4557_length_2403_cov_1.890119, whole genome shotgun sequence.
ACCESSION   DAWACS010000207 DAWACS010000000
VERSION     DAWACS010000207.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4557_length_2403_cov_1.890119"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2153..2162
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          593 a          601 c          400 g          799 t
ORIGIN      
        1 gtattcgata ataggtacat caaaaaagta atgcggttca acattttgca atgataatgc
       61 cgtatagctg tcaagacctg tgtatgtata tataaacgat tcatcataca cacgacaaat
      121 actgcctgtg cagtagccgt caaggtcgta tttcttgtag taatacaccg caaacaacgg
      181 cttttcaaat gccgactgtg agtaacatac aaatgtatgc tccggatcca atcggacact
      241 tctcggcttg cttttttcgt ctgcataaat cagttcatat gctttgccgt aaatgctcat
      301 attctttaca atttcactgt caacactcgg catatcctgt tccaaatatt cgtttttgat
      361 tgcctcaata tcgtattcgt ccgacaccgc atatgttacg ggattgccga caagataact
      421 ctgtgtcata tccgttatgt actttgcgtg attacacatt atgcggttgt ttgccacgtt
      481 tttgcccctt tttctgcggt ttaaaatgcg gtggtcgccc atatagtaat cgtgcaataa
      541 tcggtatctc tgtcgctctc gctcgtgccg ttcaatcaat ttcgttatga taaacggtgt
      601 cacaccgcct gcgactatat cttcatcaat tatcatattc cgtactcctc tcttgaatag
      661 attttagctt tcttatcctt gcgccaactc tcaacgccgt atctcagtgc cgccattgcg
      721 tcatcaaata cattgacagg ttcgtcagta tactcgcccg acttttcatc aactcgccaa
      781 cgccattgct gtatctcttt gattacattc acgcaagacg gatgaatatg tatctttctg
      841 ccttttaacc agtcaatctg cgattgtatg ctgttcggat ttttaacaac tgcccttgct
      901 cgatagcctg cctttcgcca catttttata cggtccggct ctgcactgtc gcaccacatt
      961 gcaagacttt tactgaactt accgtcagcc ttagtgataa tttcggtcgt atccatttcg
     1021 tgtacataca gttcattaca aacgtaaata tcgccgtcct tatagcctaa cgtcaatata
     1081 gcatttgcgt gattaaagcc gaagtcctgt cctatcgcca tagcgtcaaa acggctcata
     1141 tctgtatcaa attcctcaat gcgataattt gagaatatca atccgcctgt ttcgccccat
     1201 tcgcccaagc cgtaaatcct gtagccctca gggtcaacct ctttacgtcg taacatacgt
     1261 tgtcgatacg cctcatcaca aaatcggttt gttaaatatg tgctttgatg cgttaagacg
     1321 ttatcgtcct gtatatcgaa aaacactttc tttatccagt gacttgacga tacagggtta
     1381 aatgtcaatt ttatctgata aaaaagaccg tcggggagtt cacctctcaa acggtcatct
     1441 ataatttcaa aatcctgttg cacaagctcc gtagcctcct caatccatac atcagttaat
     1501 ttaccgtttg caaatgtgat tgatttcagc ttttcacgtt gcttgttatc gtttacacca
     1561 cgaaatataa tcttgttgcc gtttatacag gtgaacgaca acggactttg cgtaactcgc
     1621 cacgctctgc ctacgcccat acggtttata gctgattcaa gctcggcaaa tgtactgtca
     1681 cggtttgtta tatcagattt acgcacacat accaaattac gccctttgtc acgcattaaa
     1741 cggagtatgt ataactgtgc agtatcaaca ctcttgccac ttccggcact gcctttcatt
     1801 acaacataac gctttttaca ttgatgtaca ggtttgaata tcggattgaa cggtactgtt
     1861 actttgttca ttcgtcctca ccaccgtaat caattttaat gctgtagtcc atatcaccgt
     1921 caacgtttaa tttgtctgtg aacaatgcgt agtatttacc caacatttcc gccgctttgt
     1981 ttacgtcaga cacctttgtc ggtatttcaa cgcatatcgg ttgctccgcc tcgtcagtga
     2041 ctttcttgcc cttgttgtca tagtgtgatt tacgtgcttt gcacgtcaca acaaccgttt
     2101 cgggtttctc acgtcgcata acagccgtaa gcgttttcaa tacctcatct tgnnnnnnnn
     2161 nnctttctct tttagccgct tttgaatata ttcctgaatt tcaggtttct tcaagttctc
     2221 attcccaatc gaatacgccg tcttttccga atatcccgct cttaacgccg cttgcgttgc
     2281 attcaaatca atcaaatatt cctcacaaaa caacttttgc ttttcagtca ctcttatcac
     2341 ctcactttca cattttccga ttaattacat cgtatatccg ttttttgtca acgcacgttc
     2401 taa
//
LOCUS       DAWACS010000208         2402 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4559_length_2402_cov_2.169578, whole genome shotgun sequence.
ACCESSION   DAWACS010000208 DAWACS010000000
VERSION     DAWACS010000208.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4559_length_2402_cov_2.169578"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    234..243
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1791..1800
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          806 a          393 c          574 g          609 t
ORIGIN      
        1 acataaaagc acaccaatat ttattgatgt gcttaaggaa atgattattt agttggtgat
       61 acagtaagct taagaccaag cggctgcatt attttaagca aagtatcaag atttggagtt
      121 gatttatacg attcaatacg tgcaactgat gattgcggga ttccgcaaag cgaagcaagc
      181 tcacgctgac ttataccgag agcggtccgc tgttctatca gtgcaccgac aatnnnnnnn
      241 nnnagtgata ttccatcccg attactcgag ggtaaattaa ataaatagat ttttagcatc
      301 tcattagagg tgcttttttt atgggaggaa atatgggggt tataaaatca atattcaaac
      361 caagagacaa gcctaaaaat catacgggtg acagtatcgg aggaggacgg tcgtttccgt
      421 tcgggcgaac gtggtcggga aaggctgtga cggaacggtc ggctatgcag acaacggcag
      481 tatatgcgtg tgttcgtatc atatcggaaa cggtagcaag tctgccgatt catctttatg
      541 aatacacgga cagcggaaaa gagcgagcct ttacgcatcc gttgtacaga cttctgcacg
      601 atatacccaa tcctgaaatg aacagtttca taatgcgtga ggttatgatg tcacatctgc
      661 ttttatgggg gaattcgtat tcacagatta tccgaaacgg taaaggtgag gttacggcac
      721 tttatccgct tatgccggaa aagatgcgta tagacagagg tgcggacagc aaaatatatt
      781 acacatataa cagtgataag caggggacat ttgtatttcg caaagatgaa attctgcata
      841 tagtcggact gggatttgac ggacttgtgg gatactcacc gattgctatg gcgaagaatg
      901 cgataggact ttctattgct gccgaagaat acggctcaag ctttttctca aacagcggta
      961 caccaagcgg agttttggaa catccgggag ttttgaaaga gcctgaaaaa gttcgtgacg
     1021 catggaatga cgcatacggc ggaagttcaa atgcacacaa ggttgcagtg ttggaagaag
     1081 gaatgaaatt caatccgatt tcgataaatc ctcatgaggc acagtttctc gaaacaagaa
     1141 aatttcaagt gaatgaaata tgcagaatat ttcgtgttcc tccgcatatg attgccgatt
     1201 tggaaaaatc aagttttaac aatatagaac agcagtcgct tgattttgta acgaatacaa
     1261 tccggccgtg gcttgtgagg atagagcaga caatatttca gcagcttctg acagaagaag
     1321 aacagaagaa atacttcgta aaattcaatg ttgacggact tctgcgaggg gattttaaaa
     1381 gccgtatgag cggatatgct atcggcagac agaatggatg gtacagtgca aacgatataa
     1441 gggaattgga ggatatgaat aaaataccta aagaacttgg cggtgacaga tatttgtgta
     1501 acggcaatat ggttgatata aataatgccg gaaattacaa cagcgggggt gaaagtgaaa
     1561 atgagtaaat tttggaggct caagactgtt aaaaacaaaa tagacgaaga aaatgaaagc
     1621 acagaaaatg tgcttttttt aaatggtgta attgctgaag aaagctggta cagcgatgat
     1681 gtaacaccga aaatgttccg tgatgaactt aatcggtacg acggtgatat tacggtatgg
     1741 ataaacagtc cgggcggtga ctgttttgcg gcaagtgaaa tatatacggc nnnnnnnnnn
     1801 gccttaaaag agcataaagg caaaatcact gttaaaataa acggcattgc ggcaagtgcg
     1861 gcatctgtaa ttgcaatggc tggggatatg gttgagatgt ctccgacatc gatgattatg
     1921 atacataatc cttcaatgat gctttacgga caggcatcgg aacttgaaca aggtattgat
     1981 tttcttaacg aagtaaagga atcaattata aatgcttatc agataaagac cggactgtca
     2041 cgaagcaaac tgtcacattt gatggacgga gaaacatgga tgaatgcaca ttcggcacat
     2101 gatatggggt tctgcgacaa aatcctatac gacaatgatg acagcactga taatcaagat
     2161 atgatttttg acaaaacaac aatggtgacc aataccattg ccgcaatgcg taagaaactt
     2221 aagctgatag tcaagccgga agattcaaag tattgtattc cgtcagaaca gtttgaaaca
     2281 agattaaatt tattgaaata atgggggtaa tataaatggc gtcaataact gatttaagac
     2341 aaaaaagagc agcgttatgg gaaaagacaa agaaatttct tgataatgca aaacgagaaa
     2401 ac
//
LOCUS       DAWACS010000209         2399 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4569_length_2399_cov_1.716724, whole genome shotgun sequence.
ACCESSION   DAWACS010000209 DAWACS010000000
VERSION     DAWACS010000209.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4569_length_2399_cov_1.716724"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1110..1119
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          882 a          321 c          530 g          656 t
ORIGIN      
        1 caatttcatt atatggcgaa gcgaacagag gaataaaacg tcactctctt gaaacaaata
       61 tagtagtaat acgtcggcaa aaggtgatag ttgaggatta tattgatggc ttgaattatc
      121 tggcggaaat atatgatgat aaagaactat tattatttac acaagaattt aaagagagcg
      181 atatgtatgt acaagctttt gaaaagtctg ttttattagc tcaaaagaga aatgtttcaa
      241 gtgatgtctt attaaaggac aaatcagata ttgattctta ttttatgggg gaacgaataa
      301 atgaaaggta aaaagaaatc caaattagag ttggttcaag aaaaagcgca agctgcaata
      361 aacaaaacaa atgattctat agaagaactt ggtaaacaca caagctgctt gtatgattca
      421 ttaacttcta ttcaagattt atttgataaa atcagaaatg taccaactga taaaaaatcg
      481 cagtatgaag aattaaagaa gataaggttg aattggaaac agcaggctga gaaaattgaa
      541 aaagattatc aagccgctgc agtgaaaaat gcgggagcag gagcagcagg agcgggtctg
      601 ggagttgctg tggttactat gggaccgact gttgcaatgg gtgtagccac aacttttgga
      661 gtggcgtcta caggaactgc aatctcagca ttaagcggag cggctgctac aaatgcagca
      721 ttagcctggc ttggcggagg agctcttgct gttggcggag gcggaatggc tgcgggagaa
      781 gcttttcttg cacttgccgg tccggtcggt tgggctattg caggagtatc tcttgttgtt
      841 agcggattat ttttctggaa aagctccagt gacaaaaagc gtcttgaaag catttttaca
      901 gctataagtg agcgagatat aaaatcctat gaattagcta ttgttgaatt gaaggaacgt
      961 atatctcgta ttattgacga aaatgctaag ttaagtgcgg caataacaaa tatagctaca
     1021 ttcggtttgg attacagaaa aatgacacaa gcacaacaat atgagcttgg ggcttatgtc
     1081 aatttgatga atgcttcaac acagctgttn nnnnnnnnna ataggtctta caccaaaatt
     1141 ctctacggag gattttaagg gatttgttgc atggaaggat agaaaaacaa atgacagtat
     1201 ctgcagagag aacaaggact ttataatttc actggccaat cttttataca agattgaact
     1261 gaatgataga gataaaaaac tcttgtggaa atctctgcga aagaacaaga agatgctaaa
     1321 atctatggat atatccaaga aagagtttga tatggatttg atgaacggca ttcttgacgc
     1381 actgagatat agatatacaa aaacactacg agtttgagtg tcatacatag ttagacgggt
     1441 aaaatgtatg atttccttat cgttaaaagg ttagtggaca agtttcaccg tacatacagg
     1501 agtataggac gtactttata tcgtgttgtg ccatgtggaa acggtttgtt taatgaaaaa
     1561 taaatattaa accaaaggag aaaaaaacaa atgaaaagca cggaattgtt aaatacaaaa
     1621 gtaattcata agatgttagg cgaggggatt ataacagaag caggagataa ttatgtgact
     1681 gtaaaatttg cctccaaagt atcaagattt atttttccta tagcttttga aaagtttatt
     1741 acggcagaaa actcagatat acaagagatg attattgaag aaattaacag agccagaaat
     1801 gaagaacata aaaaacaggc agaaaaggaa atggttcgta agcaagaaag aacgcgcatt
     1861 aatgccgaaa agaaagcaag tgctaataaa aaatcagttg ccagaccaaa acgtataagc
     1921 ggaaaacgta tgattttctt tgtttttcaa ggaaatacat ttgataagga gttcaatggt
     1981 gggtacatat gggcaccaat ttcaaataaa gcgggaaata aatttcatca ctggaaaaaa
     2041 ctacttgatg taagagcggg tgacatcatt ttgcacggat gtgacggata tattaaagca
     2101 ataagtgttg caaaaacatc gtgcttcgat tgtgtccagc cagaagaact ggtagttgaa
     2161 gatttatggg atagagaagg gcgtaaggta gaatgtgatt atgtaaaaat tgaaaatcct
     2221 ataaaaacat cagactttaa agatgatatt attaggtttt gtcaggttaa atattcaccg
     2281 tttgataaag acgggaatgg aaatatggga tatttgtttg atataaaccg agaacttgca
     2341 aaaatattta tcagagcaac tgtcaataaa aataattatc ttaactcaat agattatat
//
LOCUS       DAWACS010000210         2396 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4576_length_2396_cov_2.123452, whole genome shotgun sequence.
ACCESSION   DAWACS010000210 DAWACS010000000
VERSION     DAWACS010000210.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4576_length_2396_cov_2.123452"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          864 a          341 c          519 g          672 t
ORIGIN      
        1 gtaaatgata ctaatgcact tacaagcggt gtcggttgtg cggcgatatt cgcgggtatg
       61 ggaacatcac tccctaatac gattgtgaaa aataacgttg tacaaaattg cgttgaaacg
      121 ggtatcgaag gtccgtatga attggtttat cacaatacag taaaaaatac aggcgaaaat
      181 tccgttgcgc gttataccgg ctctacagag gcgatatata ttaagctgac gactgaattt
      241 gaacagaaat atataggaaa tactatcgaa acaagaggtt tgagatgctt ttcttcttac
      301 tcgaacagag atgatgagta caagggaata tacattttga acaattctat gaatttgaaa
      361 aatacagatg caagtatcgc ttgcaattac actcgttcgg acattgaaat caactgtaaa
      421 aaaataaaga agattgttat tgaaaataat acgggaatga tgaaagacaa aaagagtgta
      481 aacatctata ccgacaaggg ttatgttatg gattatttca gtattcataa tccttgtatg
      541 ataggctcag tgcctgaaaa ggcgagatat tgctttaata tcaataataa ttaaacagaa
      601 tgcagtcaaa atcgttgcag atttgtctgc agactcaatt aagctaatca ttggggaaca
      661 cataaacaat gcgtatgctt tgtttatgtg ttcctttttt ggtgcaaata tataaaaatt
      721 ataggtatat ttaatagata attactaaaa agtaaaatat atgttgaaat tgcacgaata
      781 aagtgatatg ataaaagtat aatttgtaca aaaaggatga ttgctatgga atatattgga
      841 catacatcag aggacggacg gaaacagctg cttttggacc acttgaacgg aacgtcgaag
      901 ttatgtcgtg aaaatgcgaa tgaattttgg gcggatatag ccgaatttgt ggggcaaata
      961 cacgatatag gcaagtatac atcggggttt caaaaacgta taaacggtgc ggaaaatatt
     1021 cgagtggaac acgcaatatg cggtgcaaaa gaagttgcga aagcaccgcc gaaatcatat
     1081 gtgcctatga ttgaatattg cgtttcggga catcattccg gtttacctga cggcggaacg
     1141 aaagttgacg gtgaagaaga ttcgaccttg cacggcagaa tgaaacgaaa aaccggcgat
     1201 tattctgcgt atgaaaatga agttaaactt gaatatccca aagataattt gagagaactt
     1261 tttgatgtca gtaatcagag agaaattatt gaaaggtatt ctttttttac aagatatttg
     1321 ttttcatgcc ttacggacgc ggattttata gatacggagc ggtttgtaac tcccaacaca
     1381 gacagaggta tagacggaga ttttcaaaaa gcatacgaaa aagtatgcga aaaacttaat
     1441 tcttttaaaa tagaaaccaa acttcaagaa tcaagaagta ttatacaaga acaggtttat
     1501 aaaagcgttg aatcgaatgc gaatgtgtat acattgaata tgccgacagg cagcggaaaa
     1561 actctttgca gtataagagc ggctttgaaa actgcgatag agaataagaa aaaacgtatt
     1621 atatatgtaa ttccgtatgt cagtataatt gaacagacag caaaggtatt cgaggatatt
     1681 ttcggtgatg tacttcctgt gttgcagcac cattcaaatt atgattttga tgatgataaa
     1741 aatgaagatg agaacgaaat aacttcggaa aaattaaaac gttcctgtga aaattgggac
     1801 gcaaaactta tagtaacaac gaatattcaa ttttttgaat cactgtatca ttataaaggc
     1861 agacgactta ggaaacttca taatctggcg gacagtgtta tcatatttga cgaaatacat
     1921 atgctgccga ttgactatat tcagccttgt ttaagggcta tagggtatgt gacgaagtat
     1981 cttaacagta cggcaatttt gatgtcggcg acaatgccga attatgataa atttatggaa
     2041 aggtatatgt cagatgtcaa aatagaaaat gcggttaagg atacgtcgtt gtttaacgta
     2101 tttgataagt gcagatatga gtatatcgga aagtgtgaac ttgcttcact ggcagaaaag
     2161 gcacaggaat atgacaatgc acttataatt gtgaacaaaa ggaaaacggc aagagaatta
     2221 tatgatatgt gcagcggtaa caagtatcat ttgtcaacat atatgacgcc tttgcaccgt
     2281 tcggagatta ttgcaaaaat aaaggaagat ataaagaacg gaattaatac gaccgtgatt
     2341 tcaacatcac tgattgaggc gggtgttgac cttgatttta aggcggtatt cagaga
//
LOCUS       DAWACS010000211         2373 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4624_length_2373_cov_3.062554, whole genome shotgun sequence.
ACCESSION   DAWACS010000211 DAWACS010000000
VERSION     DAWACS010000211.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4624_length_2373_cov_3.062554"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          600 a          595 c          412 g          766 t
ORIGIN      
        1 taccgctgta ttcctttgac gcgataacct gttcacgtcc tgtacctgtc cacaatgtaa
       61 acgtaatcaa attatctgta cacttctctt ttatcacatc cgtcacatcg aacgatattt
      121 ccttataatc gacattgtat gcaggtgaca cttgtaaacc caatgacgat aacggcgtac
      181 cctcgcctct tgtctttata tcatttatag taacggtcgc ctcatcccaa tcattttttg
      241 aacaataatc gaggtgcaaa accgaatttc tcttatctgt attagtaaat ttatcgtaca
      301 atttcaatgt tgccgaagtc aattttttac cggcatattt agaaaggtca aaacgtatca
      361 accccaaacc tttaatatcc tttaatcccg gagatgttga cgagtaataa tctgtcgcct
      421 gattaatttt taattcttca tcagatccat gtgctgttgt accgtcaaca agatacgtgt
      481 ctgcgtccgc agtgacagaa accgaaacag gttcaggcgt tgtcttgatt gtcggacgcg
      541 gtgtaattga cgatgtgtca ggataagtca cctccgtaaa tgacgtattc ggtgcaaggt
      601 agtttaacgt gttgccaccc gaatctttcg ccaaagccgc cgaacctata taatagcttg
      661 tatgaggcga ctggttgtat ccgacattct gccatgctat tgcgcaacga tattgactgt
      721 cgtgcataag cgttgtaagt ctgtaatcag tcggaatagt cgaaaaataa attctcacac
      781 cgtcaccgta tctaagcacg atttcttcac gccaatcacc gaaaaggtcg gctgtaagac
      841 acgcatttga ctttgtactg ttatttattg aacccggcaa tgttgaattt ttactgttat
      901 aataaattct ttccgtaccg ctttttatgc tgtattttac aagtttattt ccgtcaagca
      961 attctcgtcc caaatcaccg tcccaataaa ctgcaaaatt ctccatattg cttccattgg
     1021 tattaggtat attccctact ttttcacctg cttgattata tataccgtca gccgctaccg
     1081 accagaacaa cgaaagatta ccgcttgaca gtgcatattt atcatcgaca ttatccataa
     1141 tacctctgcc tatatctccc tgtgcggctt gtagcataat atcaccatta tatctcttta
     1201 tatctaccgc atatggaatt gtgtcatcat tgtgcttacc tgcctcgtgc gccataaata
     1261 tttcttgttt tccatcgtta tcaaagtccg aaacgtgttc cgcgtcaccg tgtccccgac
     1321 cgtcatacgt caaaacagtt ccgtcatggt caagaaccgc actgccgaaa ataatctcgt
     1381 catatccgtc attatccacg tcagctaccg atacactgtg tgcaccttgt gcataaagcg
     1441 ttttgtttgt attattttta cttgtaccgt ctgcatattt tacggtacaa cctgtaccgc
     1501 catttgcttc tgtcggtgta ttagttgaaa gccattgttc tttaaggtct gtgccgtccc
     1561 aagtatatgc cgccgcatac acatatgtat aatatcccct cgtcataaca acgcttggat
     1621 gtacaccgtc aagatatgcc accgcggcaa ggtatctttc acttctgttt gcatagctgt
     1681 cgccccattc ggaagatgtc aaaatacttc tcggcgggtc aaaatcaaca gtctgcattg
     1741 ccgcgcctgt ttcaccgtta aatacagtta aatactccgg tccgccgtcc tcgcccataa
     1801 gcaaaccaga tgaatttctg taatcttttt tatcgtcacc cttggttata ttctttcctg
     1861 cttttgagac ataattaccc tcaccgtctt ttgagcccgg tgctgttttt aatatgactt
     1921 ccgacttacc gtcaccatca aaatcataca ccatatattg agtatagtgc gcacccgcgc
     1981 ggatattaac tcccaaatct attctccaaa gaagagtgcc gtcaagctca tatgcgtcca
     2041 agtacacatt gcccgtataa cccttaacgg aattatcctt tgaattagac gggtcccatt
     2101 ttacgatgta ttcatattct ccgtcaccgt caaggtcgcc cacgctcata tcgtttgcgg
     2161 tatatgtata ttcttcatct gtcggcgtct ttcctccgtc aggaacttta aacgcaatgt
     2221 ctttatatgc cacagaacga cctttatatg ttgtgtttgt tgtccaaaca tcaaccgcct
     2281 ctgttttgtc tattgcctca cctttcggca caactgtata cttgttatcc gccgtgcctt
     2341 ttgaatcggt atagcaagtt gcatcgtgtt cgc
//
LOCUS       DAWACS010000212         2363 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4644_length_2363_cov_3.051993, whole genome shotgun sequence.
ACCESSION   DAWACS010000212 DAWACS010000000
VERSION     DAWACS010000212.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4644_length_2363_cov_3.051993"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          826 a          397 c          534 g          606 t
ORIGIN      
        1 gtgtacactg agtacacggc agtttgaaag aagctttgga ctgcactcaa atctgaactg
       61 attaaaatag ccgagaggtt atatcacagg atactaattt atatattatt ttgcaaaacg
      121 cagtgaaaag cactgcatta aaatttgttt atgacttgtt caggcgaaca gcctgaatga
      181 gttttaatac aacactataa aaattttttg atgatatctt ttaatggagg tgtagaaaag
      241 tatggcaaga atagcaggtg ttgacttacc tagagaaaag agagtagaga ttggtcttac
      301 ctatatatac ggcattggac taaagagttc tcaaaagatt ctatctcaaa caggtataaa
      361 cccagacaca agagtaaagg atcttacaga agcagatgta agtaagctaa gagaagttat
      421 cgacgctgaa tacacagttg aaggtgacct tagaagacag ttggctcttg acatcaagag
      481 acttatggaa atcggatgtt acagaggtat ccgtcacaga agaggtcttc ctgtaagagg
      541 acaaaacaca aagaacaatg caagaacccg taagggtcct gcaagagcga ttgcaggtaa
      601 gaagaaataa aggagggact aagctaaatg gctaaggtag caaagaagac tacacgtaca
      661 agacgtcgtg atagaaagaa tattgaaaaa ggcgcggctc atatccgttc aactttcaac
      721 aatacaatag ttacaattac agatacagct ggtaatgcta tatcttgggc aagtgccggc
      781 ggtttaggct tccgcggctc aagaaagagc acacctttcg ctgctcaaac agctgcagaa
      841 acagcagcta aggcagctat ggaacatgga atgaagacag tagaagttta cgttaagggc
      901 cctggtgcag gtagagaagc tgcaatcaga gcacttcaag caacaggtct tgatgttaca
      961 atgattaagg acgtaactcc tattcctcat aacggttgta gaccaccaaa gagaagaaga
     1021 gtctgatttg tatattattg taaaggagga aaaattaaca tggcaataaa cagacaacca
     1081 gtgttaaaga gatgcaagac tttgggcatt aaccctgcaa caatgggtat tgataagtca
     1141 tcaaacagaa acccaaagca aggcagaaaa aagcagtctg aatatggctt gcagcttaat
     1201 gaaaagcaaa aggttaagtt tatatacggt gtacttgaga agcaattcag aaagtactat
     1261 gtaatggcta caaagaaaca gggcatcaca ggtgaaatgc tgcttcaaat ccttgagtca
     1321 agacttgaca atgttgtttt cagattaggt ttggctaata caagacgtga agcaagacaa
     1381 attgttaatc acggtcatat cacagtaaac ggtcaaaagg ttgatattcc gtcatacctt
     1441 gttaagccgg gtgatgtaat agcagttaga gaaaagagta agaactctgt tagaattaag
     1501 gaaatcgttg aaacaaacgc aaacagggtt gttccaaagt ggctttcaat ggataagaat
     1561 acacttacag gtaaggttat cacattggct gcaagagatg acatcgacta cgaagttgag
     1621 gaacacctta tcgtcgaact ttattctaaa taattatatg ttaccctcgg tacgtggata
     1681 tatatatatg ttttttcgag agatttcgga gcttagactg ctccgaagtc atctctaatt
     1741 atgaaggagg gttctgaaat gatagaaatg gaaaagccta atatagaatg cgttgaaatg
     1801 agtgacgacg gcagatacgg caaattcgtt gtatctcctc ttgagcgcgg ttacggcaca
     1861 actcttggta actcactacg cagaatttta ctttcatcat tgcctggtgc ggctgctact
     1921 tcaattaaaa ttgaaggtgt gcagcacgaa ttttcaactg tacccggtgt tacagaagat
     1981 gttacagagc ttatacttaa cattaaaaag cttgctttga agattcacgg tgatcttcca
     2041 aagactgttt acattgaggc aaagggtgca caggaaatta cagcaggtga cataaagcgt
     2101 gacgatgatg ttgagatttt taatccggat cttcatatcg caacacttaa tgatgatgca
     2161 aatctttata ttgagattac tctatcaaag ggcagaggct atgtaagtgc tgataagaac
     2221 aagcaagcaa tgcagccggt aatcggagta atccctgtgg attctatcta tactcctgta
     2281 acaaaggtta attatacagt agaaaacact cgtgtaggtc aatatacaga tagagaacag
     2341 ttggcagttg agctatggac aaa
//
LOCUS       DAWACS010000213         2363 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4648_length_2363_cov_1.665511, whole genome shotgun sequence.
ACCESSION   DAWACS010000213 DAWACS010000000
VERSION     DAWACS010000213.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4648_length_2363_cov_1.665511"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          797 a          382 c          524 g          660 t
ORIGIN      
        1 gcgaccttgt tgttatggaa aaattcccgc ataccgcaaa tgacaagatt gacagagtta
       61 cactaaagaa atctttggga taaaggaaaa gctatggaaa caatgatgca aagtgccgta
      121 tgccttttgg agcaggcggc ggaaaagttt aaaaataaga cagtatttga agataaggac
      181 ggaagtattt cttttgagga attgagagaa aaaagccgta taataggaag tgaacttatt
      241 aaaagagttg aaacaggcag aaaatgtcct gtaatcgtat atttgccgaa aagcataaaa
      301 tcaataatca gctttatggg ttcgctttac agtgcgtcac cgtatgtgcc gattgattac
      361 gcaataccga tggcgagaat ggaaaagacc gttgaaaatc ttcatccgac cgcgataata
      421 acagatacgg acggcaagac aaaacttgaa agtcttaatc ttgacacaga gatacttata
      481 tttgatgagc ttgcagacgg acaagccgat gatgcggcgg ttgataagtc gctgggcggt
      541 gtgtgcgatc ttgaccctgc gtatattatg tacacatcag gctcaacagg tgtgccgaaa
      601 ggcgttacaa tttcaaacag aggtgttata gattatattg aatggattgt cgaaacattc
      661 ccgattgacg aaaacagcgt aatcggtatg cagtcggcgt tccattttga caattctgtt
      721 tttgatatgt ataccgcttt ttatacgggt gcaaaaacta tgataattcc agagatactt
      781 tttatgtatc ccgaaaaact tatggattat atgacggaaa acaaaatttc cgttatcttc
      841 tgggtaccaa ccgttatgat aagcgttgca aacggcggtg tgcttgaaaa tgccgatttg
      901 agtgcattaa aactgatatt gtttgcaggc gaagtaatgc cgaatactca gcttaatata
      961 tggagaaaat atttgcccga ctgtatgtat gtaaatatgt atggtccgac agaagcaacg
     1021 gatattgcaa cgtattatat tgttgacaga gaatatgaaa atcacgaaac tttaccgata
     1081 ggtaaggttt gcagaaatat gcgtgcattg attttgaacg atgatgataa gcaatgtgct
     1141 gtcggtgaac aaggtgaact ttgcataagc ggtacgggta ttgcactcgg atattggaat
     1201 tcaccggaga ttacggcaaa agcatttgtt caaaatccgc ttaatacaaa atatcacgac
     1261 agaatttacc gtacaggcga ccttgtttat gaaaatgaag aaggcaatat aattttcata
     1321 ggcagaaagg acagccaaat taaattgaga ggtaacagaa ttgaactcgg tgaccttgaa
     1381 agtgcggcgg cggctattga aaatgtcgca agtgcatgcg cattgtttga cgcagataaa
     1441 caggagattg tattgtttat cgaaacaaca gcagaaattg ttgcaagaaa gtttaagatg
     1501 gagcttaaaa agtatgttcc ggcatatatg gtgcggggca aaatcgtaac tatggaaaag
     1561 ttccctcaca caccgagcgg aaagatagac agagtcggtt taagaaaaac ttatattacg
     1621 gctgagtaaa attgcccaca gcactgtgaa caattttctt tcagccgaaa caaggaaatt
     1681 aaaatcaaaa aaggagataa ttaaaatgga agaaatcaaa gaaaaactaa gagcattcat
     1741 acaggagaaa tttgaaattg atgatgatcc tgattttaca gatgatgtac atttgttcaa
     1801 tgaaggtttt gtcgattcat tcggcgcggt tgaaataatc cactttatcg aacagacata
     1861 caacatcgaa atcacacaaa aggatataac actttatccg atgaacacaa tcgaagaaat
     1921 agctgaggtt gtcgaaaata aattatcata atcagaaagg taacggataa atgtggtatc
     1981 ttcagacaga tgtactttta agacttttta tagcctgcgg tgcaatgata ctgatttcag
     2041 gactttcaag gctgattttc aaaaaagatt tcggacctaa agttcttgta tttgcaagta
     2101 tagcagttat agcatatgtt tcgtggcagc ttgccatagc gattgtatgc tatacactta
     2161 taacatttat tttcgcacga atactgaaac cgctgaaaaa aacacggcgg tttttcttcg
     2221 tactgttcag tattttgtgt acagtgccgt ttttctacgg acgaatgacg gagttttttc
     2281 cgcaacttcc tgtaataatt acatttgtgg gaatagcgta tcatatgcta aaagcggttg
     2341 acgttatgta cttcgtgtac tat
//
LOCUS       DAWACS010000214         2352 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4683_length_2352_cov_1.932956, whole genome shotgun sequence.
ACCESSION   DAWACS010000214 DAWACS010000000
VERSION     DAWACS010000214.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4683_length_2352_cov_1.932956"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    743..752
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          586 a          552 c          394 g          810 t
ORIGIN      
        1 ccgcagtata gtccgttcct gtccattctt ttcttgtaaa tttatctgcg attggattaa
       61 taccattatc agctgctgtt aaggacggga attccgcttg attgtcccac ggtgtatcaa
      121 cattaggcat atcataaatt acatttgtta gggggcgcat attgctgtcc catacaaata
      181 ttttataata ttcaccgttt tttaagcctg acggatatat attcgtaaac tctgtctcat
      241 cctttattga ttttttaatt tttttaacat tttttaatgc tccgtccgca ttgtataccg
      301 cagtataaac gaaaatatcc cgttcacttt cgctcgccac atatatgctc tcattttcct
      361 gatagcactt aacatcctcg acaatactta tattgtccaa acgctctgca tacgaggtta
      421 ttcccgtaat agccaacagc acggccagca ctaaaaataa ctttgttttc atttttgttc
      481 tcctttgata tatttttata ttctaattat acaatataag tatctgtttc caaagataaa
      541 aaccaggctt gttggcttca ttgtcacaag cctggttttt attatatatg aatgtagcct
      601 aattaaaatg ctgtgctcat atagtttctt ttacacatct gttgttatca atcaatcaca
      661 aatgttgtaa ctgagtttgc cggtaaatca aagcggaatt taccgtcttt tacttccgca
      721 gttcctacat ctttccaatg ttnnnnnnnn nnctgtaatt ctgcgtatca ccggttgtat
      781 ttaccgcaac aattcttatt gcgccggtat ctttgttata tgcagccagc gttgtaggcg
      841 atgacgcaat gattgtatca cccggattta tatattttgt aaactgacca taaacataat
      901 acttttgggt aagttctatt tccttcgtgt catggtttac catccctact ccccaagttc
      961 cgatatgctg cgcatttgaa gtgtttgcct cggaatttgt accgtttgga gccacgaact
     1021 cagcgtcctt atgcttatca agaatatccc acattaccca ggcagagggt tgcatagagt
     1081 tcatatcttc aagtatattc actgccaagc ttaaacctgc acccatattc ttcgcatttg
     1141 ttccggctaa gtttccgtta tcaacctcgc tcatccagaa gttctttccg ctattcacag
     1201 ccgtttgctt caatgccgca aagttttcac tactgcggtc actatatgta tgtgtatcaa
     1261 atctacccaa tgctgctttt gcttcagttg tcagcgcctc atatgcagtt tttgaatcca
     1321 cgaggctcgt ttcatccggt ccgacaatca tagtatctat gttacgtttg ttatactcgt
     1381 tatccaacgc aagaataagc tgtgattgat gcacgccctg agcgacatga tttccctcct
     1441 gctttgcatt cattgccaac cagtattttc ttgccggatt tggctcattc ataggtgaat
     1501 agctgtcaaa ctttaaacca atctcatcat aatgttccgt tacatctgcc agaaataccg
     1561 caaactccgc gtatcttgat gggtcaagat tttcggctcc gccaactccg ccaccggagc
     1621 atttgctgtt tgtcataaac cacggcggag aatttgtgta tccttcaata tgtacatttg
     1681 cattttgttc ctttattttt tgcagcacat ttgtctggtt cttatcggca ttccagttat
     1741 agtcgtacat ataacatccg ttttcgtccg tcttaaatgt tccatcctca ttatactgcg
     1801 gttcggcaaa gcacggaagc tttgaatccg agcgcgtaac gtgattatgg gtcggatcgt
     1861 cgccgccgcc tacattatat cgaacaatat ccaagcctaa accatcttca ctgtaaaaaa
     1921 gttccgcgct gtcctgtgca agtttatcgc tataacctat acggttgccc caccagccca
     1981 tactagttcc ccaaccttca aatacaccgt cattaaaagg cgattcttct tccggattga
     2041 gttttactgt tatactgtca ggtgcatagt caattaatct gtcatattcc gactgcgtta
     2101 taggtataac cgtaccatgt ctgtatttcc cgtcaaagct gaaatttgtt ccggcagtaa
     2161 atatgccttt tgaaatatca tctgttatga acggcttata gccttgattt ttcgaataat
     2221 aatcaagaag cagacaccat ttattttcac cgttaatctt atatgctgtc ggtccttcat
     2281 aaccggttac gctatttcct gctttaccgt caattgtgta tgtgcctact gcctcaaaaa
     2341 ccccgtcaag gt
//
LOCUS       DAWACS010000215         2349 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4687_length_2349_cov_2.461639, whole genome shotgun sequence.
ACCESSION   DAWACS010000215 DAWACS010000000
VERSION     DAWACS010000215.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4687_length_2349_cov_2.461639"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          755 a          505 c          374 g          715 t
ORIGIN      
        1 gacggcaacg atgatatgtg cattaaaata ccgctttttc tcatttattc tacctcccga
       61 taagttgtaa aattacatgt ggattttccg caatatagtc tgcaccggct tgtaccaatt
      121 catttcttgt tctgaatccc caaagcactc cgaccgacgt catttcggca tttttcgccg
      181 taaatatatc aacattggtg tcacccaaga acagacactc tgacggcttt acaccgagtt
      241 ttttcgctat ttcaagcgcc gtaaaaggat tgggttttgt cggcataccg tcaataactc
      301 catgcaccgc gtcaaaataa ttttcaccga aaatattatc ggtaacaaaa tgcacgacat
      361 tatccggttt atttgagcat accgcaattt ttatgccgtt ctctttcagt tcttcaggca
      421 attctcttat tccgtcatac gcgtccgtat cataaagata atcattttca taattttcgt
      481 catacacatc acgcactttt tcaaacattt ccgcattatc cttgccgtaa actccgagca
      541 ttctgtgaat aagtttatct ctgccgtcac cgacatatat tttataatcc tctaccggaa
      601 tttcgggcaa accgaatgcc gcaagtgcaa tattaccaaa atgtgcaata gcatttatcg
      661 tatttgtaag cgtaccgtca agatcaaata tacacgcttt aatattactc ataaaatcac
      721 ctcattatgt taattatata tcttgaaaac tattatgtca atgtgaatag attatgaatt
      781 tttcttgata tgcttgattt ttaaaaaaca ctgtgatagt atatcattaa agcttctaca
      841 aaattgtttt ttgaagaaag gatatgtaaa tatgaaaaaa agtttaatcg ttattgccgc
      901 attaattctt gcaatgtcag ttgcgggctg cggtaaaaag acagaaaata caaatacaac
      961 acctcagcag actgcgcagg cagatgcgtc gggtgcatac gaaaacggca gtgcaacaga
     1021 aactcttgac ggtcaggact attcaaagaa tgtcgatatg gcggcagtcg aaggtacaga
     1081 ggcaaaatca gatacaaaag cggacagcta ttcgggcgaa gtaagcaaca atgatatttc
     1141 tatcgaggaa gcaaaactta tcaattatga tgacagcgac gttgttgttg tgtcatttga
     1201 atttacaaac aaaaccgatt cagaccaatc attcagcgga gtatatgacg tcatcgccga
     1261 acaaaacggt tcttcattag ctcctgcaac agtaatcggc gtggacggtg tggaacttct
     1321 tacactttca caaaatattg ctccgggtga aacaattact gttcaaaaag cgtacaagct
     1381 tgacagtaag tcgtcaccgc ttgaaataac agttcagccg ttctcttcag acgacgaatc
     1441 attcgttaca aagacattta atttctaaaa caaataaaaa atatctctgc acaatgcaga
     1501 gatatttttt attatgcttt aacaaccaca tttttaccgt taatttttcc tctgtacagc
     1561 aatttatcgg cacttttaag aagaacatca tcgactttac cgctatcacg ttcgataatt
     1621 ccgaacgtca tcgtaacgga aatattgtta ttttcataaa caaaattttt attttcgata
     1681 gtttttctta agttttcaac ttttacaaat gcactgtcaa tatcatcatt acagaacata
     1741 aacacaaatt cttcaccgcc ccacctgcat atatcaacat tctccaagct gagcgatttt
     1801 ctcataatct ccgaaatatt ctgcagtaca tagtcaccgc ataaatgacc gtatgtatcg
     1861 tttatttttt tgaaatcatc aatatcaccg atacaaatga ccacagattt attgtcctcg
     1921 ctgtccttaa ttcgttccat aaacattctt cggctttgca agcctgtaag ttggtcgtaa
     1981 tttgcaagat tgttaagagt cttgttttca tcaacgagct ttctatgcgt tacagttgcc
     2041 gatacgtccg atatgtacgc acctatcaat ataattccga ctgccaaaac ggtattgtat
     2101 attgaaacaa aaacgatatg tacttctgcc tccgagtaaa tatgacggca atgcaacgtc
     2161 aaacctatat acaacagtac ctcacatatc gaaaatatat atgttacact acgatgttta
     2221 aacggattaa aatacacaaa tgttgccata gaaatcaaat acaaggcaaa atccgacgaa
     2281 tatccgccat ttagcaccgt caccgtaaca aacgccgata cttccaaatg caccagtaca
     2341 atacaaacg
//
LOCUS       DAWACS010000216         2334 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4721_length_2334_cov_1.904783, whole genome shotgun sequence.
ACCESSION   DAWACS010000216 DAWACS010000000
VERSION     DAWACS010000216.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4721_length_2334_cov_1.904783"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    550..559
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          650 a          551 c          351 g          772 t
ORIGIN      
        1 ataatgacac attacgttat ctatcataaa caacaccata aatatcggta caagcactac
       61 acgaatcatt gtaattttat tagctgtatt catcaaatca cctccgcttg acctgtaagg
      121 tcatattcat ctgcgtcaag aattttcact tttataatat cgcccggagt aacttcttcc
      181 tccgttgcaa aatatacttt tccgtcaaca tcaatactgt cgccgcgact tctgccgtag
      241 aacaagaagc tttcttcatc gtatccctca accaaaactt caagtgtttt gccaagctta
      301 gccttgttaa gttcaagcga tattccctgt tgaagtgtca taagtctgtc aagacgttct
      361 tccttgattt cctcatcaac ctgattcgga aaatcagccg caggagtatc ttcttcctgt
      421 gaatatgtga aagcacccat tctgtcaaaa cgcacctttt taacaaattc ataaaggtcg
      481 ttaaactgtt cctcagtttc acccggaaaa cctacaatta tagacgttct gattgtgcag
      541 tcaggcattn nnnnnnnnnc gtcttgccat acgtcttaaa atctcattgt tgccatgctg
      601 tacgggcata tcgatataat tgcatatttt atcatattta gccattgtat caataagact
      661 gtccgtaata gcctcgggat agaaataatg cactcttatc cacttgatac cgtcaaccgt
      721 gacaagtttt tcaagcaact tatcaagcga atattcgccg tacaaatctt ttccgtaacg
      781 tgaagtatcc tgtgcaataa gtataagttc cttaacaccg tttttcgcaa gattttcagc
      841 ttcttcaact atatcttcaa tttttctgct cctaaaatgt cctctaatca taggaatagc
      901 acaataagta cagttattgt cacaaccgtc ggcaattttc aaatatgccg tatatgacgg
      961 tgttgacaaa attcgcggaa gccgttcatc tggagttctg tcattatgac cgcatattac
     1021 aggcttttcg cccttaaacg cctcgcggat aacctccgcg attttatcat aatcacctgt
     1081 tccgactatt gcgtcaacct cgggcagttc gattaaaata tcaccgctgt atcgttctgc
     1141 caagcaacct gtcgcaataa gcaatcgaca tcttccgttt ttgtattgag ccatttcaag
     1201 tatagcgtct attgattcct gtttagccga ctcgataaaa ccgcaagtat ttacgattat
     1261 gacatcggcg tccgacggat tatacgttat attaaaaccg tcatttgcca atataccgag
     1321 cataatttcg gcatctgtct gatttttcgc acagccaagc gaaacaagac caatgttata
     1381 cataagttta ctctccatat tcttctttta tattatctat acaattttta atgtcagaaa
     1441 agccgtaacc tctgtacata aaatatcgta tacatttatc aatgtttttc ttttcaaagt
     1501 cacctttaag tcttttttca acaagaggca aaagcatatc ttcttcgtcc tcatcaagat
     1561 acgaaaccgc ctcctcaacg tattccgcat ctataccctt gctgtaaagc tcggacttaa
     1621 tacgtctttt accgtatttt ttcaaattct ttaaatcctt tgcctttgca atcgcatact
     1681 ttctgtcgtc caaaaagccg taatttctgc aaaattcaag aatttcatct atatcttctt
     1741 cctttgcacc ggctcttttc agcttatccg aaagttcacg ctcactgtgc gaacgaaatt
     1801 caagcaatct caaagccttg tctttagcct catcaaaggt cagttctttt ttttctctgt
     1861 tatatctcat aatacaaagt aatcaaataa ttctccaaaa ttaattccgc cgataacctt
     1921 atccttgtat ttgcctttca aatggtcata tccgctctgc atttgctctg acttaatgcc
     1981 atagctaagc gacatatatg cctcaatgta tgcggaaagg tggtcacatc cgcgtataat
     2041 ctgtccgtct atcggattgt atttgtcttc attatacttt tcgtttatca agtccgacgt
     2101 aaccatattg atttcgccgt catcaataat tttagagtca aattcattct gcgtgtaata
     2161 ctctatctcc ctgtgccaag caggcggtaa aagcggataa ataacttcac gcatttgttc
     2221 atcttctatt tcactgatta tgctgtccaa acccttgacg ctgttcttta ccggtgacac
     2281 aatatccctt gtcaagacct cgggtaaatc gtgaaaaagt cccgaaaaga agtt
//
LOCUS       DAWACS010000217         2318 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4753_length_2318_cov_2.788334, whole genome shotgun sequence.
ACCESSION   DAWACS010000217 DAWACS010000000
VERSION     DAWACS010000217.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4753_length_2318_cov_2.788334"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    279..288
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          635 a          568 c          367 g          738 t
ORIGIN      
        1 tatcctccat ttcatacttc aacgctcttt cggtttcacc ctcacagatt tcttttcgct
       61 cgcctgtttc ggtgcagaca atcgttgcct tacacgctct aggatattcc ataatctcga
      121 tatatccctt atcaccgcat ataacacatc ttttcggttg ttttgtatga agcgacaatg
      181 aaataaccgt catttgtccc tctttgttcg tcataacaat tcccgacatt tcatcaacac
      241 cgctcggtgc aaattttacc gttgaaatta cattgtcgnn nnnnnnnnac atcttgcaag
      301 cgaaattgcg taaacaccta tatcaagcat tgctccgcct gccaaattca tattaaaaaa
      361 tctgtttttc atatcatagt ccttgtaact tccgaaattt gcctgtatca tactcacgtc
      421 accgatttcg ccgttcgcaa caagtttcat aagttttttg tatagcggca tatgataaat
      481 cgtcattgcc tcggcaagta caagattttt ctcgtcagcc ttttttaaag ccttttccaa
      541 ttcaacgctg tttaatgtta tcgacttttc acacaaaata tgctttccgt tttcaagtgc
      601 tttcataata aaatcaatat gcgtgttatg cggtgacgtt atataaataa cgtccacatt
      661 gtcgtcataa aacatatcgt tcaaatcgtc atacaccttt tcaatgccgt acttatcagc
      721 aaaatcaacg gctttgctgt gcgttctgtt gcctacagcg taaagtttgc accccacacc
      781 ttgcattgcc tgcgccatct gatttgccac aactcccgta ccaagcaccg cccaatttaa
      841 tttatccatt atatactcct ttctcagtgt ggcattgccg catagctttt atcaatcgtg
      901 attacacagt aatatgtagg atatttttcc tttaccatat catttatcgc tttcttgact
      961 tccttgtcgg tcatatccat attatgcgga acaacgcagt caaatattac gtttgtatgc
     1021 gtaataccat acactatacg caagtcatga attgtcagac gttcgtcaat ctcttttacc
     1081 ttttcaccaa tccaaattct aaggtcgttc actgtattgt cctttgtcga aatcgggtca
     1141 aaatgcacaa tcatatgaag tccttcatca ttcataaaat cacgctcaat attatctatg
     1201 acgtcatgat ttttcatagc atcgccttcc gccgccattt caacatgcac acttgcaaac
     1261 tgtctgccgg gtccgtagtc gtgtaccatt aagtcgtgag tacccaaaac accgggatag
     1321 cttaaaatct tttgacgaat ttcttcaacc tgttcgtcct ccggtgcttt tccgagcata
     1381 ggatcaagag tgtcttttac aagtccgaaa ccgctgtaca aaataaatac cgccacacca
     1441 agacccatcc agccgtcaag ctcaaagccg acaaaatgcg ataatatcgc cgcaaccaac
     1501 actgcgcctg tcgttataac atcattacgg ctgtctgccg ccgttgctat aagcgtgttt
     1561 gagttaatca ttttacctgt tttcgtatta aacaatgcca tccacgtttt aagcaaaatc
     1621 gaaaacgcaa gcacaccgac agtaacccaa ctgaattcaa ccgctgacgg atgaagtacc
     1681 ttatccaaac tgcttttgaa cagttcaacg cctattacca taataagtac ggcaaccata
     1741 agtcccgaca gatattcata tcgtccgtga ccgtaagggt gttcttcatc tgccggacgg
     1801 cttgcaagtt taaaaccaag cagactgatt atgcttgatg atgcatcgga taggttgttt
     1861 accgcatcgg cagtgataga aacagacgcc gacagtgttc cgacaattag tttgcttatg
     1921 aacagtatcg cattgcacac tattccgact atgcttgaaa atttaccgta tgctgtacgc
     1981 acatcggaat tttgcgtgtt ttcatagtct tttataaatc tttttataag tagttttgtc
     2041 atctatgtca cccaatcttt ataacataat tattctcttt caatacgtca agtgcctttc
     2101 gaaaaaattc gttctttata agtatataat cagtgttgta cgttgatacc gcaaaaatcg
     2161 gtatttttgc atttgctaaa atgtccgcag ctctcgcaag tattcccgtg agtgaaaaat
     2221 caagtatacc ctcgattctg aacgctctcc aaccgctgtc acatacaatc gtatttttcg
     2281 gaacaaactc gcttgagcat acaagcgata attcctca
//
LOCUS       DAWACS010000218         2310 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4777_length_2310_cov_2.419512, whole genome shotgun sequence.
ACCESSION   DAWACS010000218 DAWACS010000000
VERSION     DAWACS010000218.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4777_length_2310_cov_2.419512"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    335..344
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          664 a          437 c          505 g          694 t
ORIGIN      
        1 ttttttatag atgtaaatta taataaaaat aatattatgc atgttccgga taatttgctg
       61 acattccggc acagtgaacg attaatcatt tattgagttt tggctattca aaatatttgc
      121 ggtcaagact tctgtattgt attgcctcag ctatgtgttc actttttatg ttttccgagc
      181 cttcaaggtc ggcaatggta cgagcgactt tcaaaatacg gctgtgtgct cttgcactca
      241 aaccgagatt gtcgaatgcg gcacgtaaaa ttgcattttc ttcttcgccg agcggacaga
      301 attttttaag catacctgcg tcaagctgtg aattnnnnnn nnnnattgta atctttgtat
      361 ctttcaagtt gcagtttgcg tgttttgttt acgcgttttt ttatttcggc acttgtttca
      421 ctgttttcgg tcgagcttaa atcttcataa tcaacattcg atacttcaac ttgaatgtca
      481 attctgtcaa gcaacggacc cgatattcga ctgcggtaac ggttaacctg tgtcggagta
      541 caggtacatt gtctgcgact gtcaccgaaa taaccgcatt tgcaagggtt catacttgct
      601 ataagcataa tgttgcaagg gtaggtgaga gttgcgttta ctcttgaaat tgtgacgttt
      661 ccgtcctcta acggctgacg caaaacttca agcgaatcac gtctgaattc cggtaattca
      721 tcaaggaaaa gtatgccgtt gtgggcgagg gaaagctcac ccggttttgg agttgaacct
      781 ccgcccgaca aacctgcaga tgaaatcgtg tggtgcggac ttctgaaagg tctgtttaaa
      841 ataagcggtt ggtctttagg taaaagtccg gcgattgaat gtatttttgt gacttccaaa
      901 gcttcgtcaa acgaaagggc gggaagtatg atcggcatac gttgtgcgag cattgtttta
      961 cctgttccgg gactgcctat aagaagtacg ttgtgattgc cggcggcggc aatttcaagc
     1021 gcacgcttga cgttctcctg accttttacg tcgcaaaaat caagtacatt tgaagcactt
     1081 gacgcaaaat aattggtaag gtcaatttta tgtacattaa ttttctgtat gtcgcaaaaa
     1141 tggtcgcata atgctttcaa agatgatacg ggatatatgt ttacaccttc aatgactgct
     1201 gcctcgtccg cgttttcggt tggtacaaac atatcggtaa aaccgctttt gtacgccgaa
     1261 ataaccatag gaagtatgcc gtttatcggt tgtacggtgc cgtcaagcga aagttcgccg
     1321 aaaaacgcac atttgcttaa gtccggtgca taaatctgct ccgtggcgca tataatagaa
     1381 agtgccatag gtaaatcgta tcccgaacct tctttacgct tggaggcagg ggcaaggttt
     1441 atgacaatgt gctttgcggg aaaacgaaag cctgtgtttt ttatggcaga acgtatgcgt
     1501 tcttttgcct ctttaacggc ggcgtcgggt aagcctacta tatcaaatgc gggtaaaccg
     1561 tttgaaatat ccgtttctac gcatacggaa aatccgtcta tacccgaaag tccgcaggaa
     1621 tatatgtgtg aaagcattta tatcacctca agtagttgat tttcttcggc tgaataccat
     1681 cagtttcatt gccgcaaacg taagcggtgt gccgagtatc gccgcacaaa tgtatgcaag
     1741 cgtcgaattt aagtttaaag ccgtaaaaat gaataccatt aggtattgaa atatgaaatt
     1801 cggtatatag cttatcggaa atttgagaaa ctttttaagt gtcggtcgtt ggtgaaacgt
     1861 aaagtgacag tttaaaaaga atgatgttat tatgctgact atatatccga atataaagtt
     1921 ggaacgtgag tgttcggcga ttaatcgcag tgggttgtcc ggtgcgagaa acgcattgtg
     1981 aagaatgtca agtaatgtgg ctgtgccgac cgatacggca gtgttcacaa tgcccaaaag
     2041 cataaatata acaaattgca ccgtcataaa cggacgtatt acacgcatca tattcggcga
     2101 aatacgttct tcaagacgta cgcgcaaatt tgaaagaaaa cgctccaaat taatcatctc
     2161 tcctttgaaa taatacttac attatatact aaatcacgga taaaaacaat agtattgagg
     2221 tttaaaaaat aaaaatgtaa aaattataaa aaaacacttg acaaataagt tagactgtgc
     2281 tatactatta tagcactctg agagaggcac
//
LOCUS       DAWACS010000219         2292 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4808_length_2292_cov_2.009388, whole genome shotgun sequence.
ACCESSION   DAWACS010000219 DAWACS010000000
VERSION     DAWACS010000219.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4808_length_2292_cov_2.009388"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2008..2017
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          681 a          475 c          444 g          682 t
ORIGIN      
        1 aggttagtgc aatgaattat gataaagaat atttcgtcaa gcttcttgaa aaacttgtgc
       61 tgcctttaaa acagcattac tctccgaaag gagcaaacct gtaccttggt catacaggcg
      121 ccgcatatga ggatcgcact atccctatgg agggattttc aagagttctc tggggacttg
      181 ttcccttgtg ggcaggcggc ggaaatattg acggcttctc agaaatatac gcctccggct
      241 taactgccgg tacagacccg tcttcggacg aatactgggg gggtttcaga aaaggcgacc
      301 agaagtttgt cgaaatagcg gcaatatcct atgggctgct gcttgcacca gataagcttt
      361 gggaaccgct cagtgacaca gccaaagaaa atctttcggc ttatttgaga ttaagcaaca
      421 attatgaagt atccgataac aactggagaa tgttccccgt tcttgtcaat cttgcgttaa
      481 aaagcctcaa tcagccgtat gaccagcatt taattgatta tggcctggag cgccttgatt
      541 cgttctatct cggaaatggt tggtataaag acggagtgac tgaacagcgt gattattata
      601 tcccattcgc tcttcacttc tacagtctca tttatgctaa agtctgcgct aatgacgatc
      661 ctgaacgctg cgctcttttc aaagaacgcg cagaagaatt tgctaaagaa tacatatatt
      721 ggttcgactc gaaaggacgc gctcttgtat atggcagatc gcttacatac agatttgctc
      781 aagctgcatt ctggagtgcc tgcttatatg ctgatgttga tgtattctct tacggcatag
      841 taaagggaat tattgtccgt catttcgaag aatggtttca aaatccaatt actgacaacg
      901 gcggcgttct tacaatcgga tatagatatc ctaatttaca tatgtcagag agttataact
      961 ctccaggttc tccatattgg agcttaaaag cgtttatatt actcgcttta cctgatgagc
     1021 atcctttctg gcaggcagaa gctgagcctt tgccacagct taataaaaat aagtttctta
     1081 aagaagctca aatgattatt cagcacgacg gtgacaaggt gacagctctt actccaggta
     1141 gactacatta caatattcat gttcatgtgt cagagaaata ctgtaaattc gcgtacagct
     1201 cagaatttgg tttcagtgtt ccccgctcaa acaagttttt taatcacgct gcacctgaca
     1261 gtactctctc atttgaaata gacggctata tcttcactcg taggctctcc ctaaagacaa
     1321 acgttgaaga ggataaaatt ctctctctct ggtctccttt caagggtata atggtcgaaa
     1381 caactcttat acctattgaa gggggacata tccgccgtca taaaattaca agcgattatg
     1441 attgtatcgc gcgtgatgca ggtttctctg tatcatgtgt cgaaggcaca gattgtacat
     1501 cctatggcga tgacaattct gtaacggtta aaaacaactt ttcattctgt actgtagaaa
     1561 gcggtacggg cggtaagcca gaagttgttt cattccatcc gaatacgagc ctcgcatatc
     1621 agaaaacagc tacaccgctt gttgtatatg atataaaaaa aggtgcaacg gaatttgaaa
     1681 caattgtaaa atactaaata gctctccata aatttttata aacaagacca ccgcattatt
     1741 cagcggtggt cttgtttata tgtcacatat acataattat tttattattt aagaaaaaaa
     1801 attgaaatag tcgaccatgt atgattggct atttcaattt tgtcaatata caaccttgac
     1861 gggactatat ctattaaaga aatttgtagt aaaatttatg atagatatag gttgacatta
     1921 aaataaattt taaggtacga tcactttttt gtctacttat tctatgcctc aacaccaaat
     1981 tatagaaata tacgaattct tttaaatnnn nnnnnnngta ttctgcgcta aactgttcac
     2041 ctgcaggcaa attattggaa tattcacgtt cggataagtc accatgaaac ccaacagcat
     2101 cgccgcgtcc ataccatggt tcaacgcaaa caaacggcgc atccttaccc gctggtgacc
     2161 ataagccaaa taggggcgca tttgttttta cagatatata ctccatacca tctggatagc
     2221 acaagctaat tttactcgct tgtcttccct ccacaataag cgcatctctg tcaaacattc
     2281 ctgcatttat at
//
LOCUS       DAWACS010000220         2279 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4849_length_2279_cov_1.966727, whole genome shotgun sequence.
ACCESSION   DAWACS010000220 DAWACS010000000
VERSION     DAWACS010000220.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4849_length_2279_cov_1.966727"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          737 a          418 c          526 g          598 t
ORIGIN      
        1 aagttgttac tgacgtaaag gcaaagtggc ttgaaacact ttcagatcta aatgtatgct
       61 cacaaaaggg actatgtgaa aagtttgact caacaatcgg tgcaaacagt gtacttatgc
      121 cgttcggcgg taagtatcag cttactccgt cagacggtat ggctgcaaaa gttcctgtac
      181 ttggcggcga aacaaatacg gcaaccgtta tgacattcgg ctataacccg caactttcaa
      241 gctggagcca atatcacggt tcaacatatg caatcgttga atctgtttca aaagcggttg
      301 cgttgggtgc tgattataag aagatttatc ttacattcca agagtacttt gaaagacttg
      361 gaaccgatcc taagcgttgg ggcaagcctt ttgcttcatt gcttggcgca tataaaacac
      421 agcttgcatt aggtattgcg gcaatcggcg gtaaggactc aatgagcggt tcgttcaacg
      481 accttgacgt acctccgaca cttacatcat tcgccgttgc acctgtaaag gctgataagg
      541 ttatttcaca ggaaatcaag aaagcaggct caaagcttgt tatgttcaca gttaaacgtg
      601 acgaaaacga tttacctaag tttgacgaat tgaagaaaat gtatgacaca gttcatcagc
      661 ttattcttga cggcaaagta ttgtcggcaa gcgttgttaa gggattcggt cttgttgaaa
      721 ctgtttcaaa aatgtcattc ggtaatatga tcggtgttga atttgacaag aaccttacaa
      781 atgatatgct attctatgca ccgcttggct caatcgtact tgaagttgaa gaagaaattg
      841 acggtgccgt aacagtcggt acaacaggct ctgacgcatt tacagtaggc ggagtaaata
      901 ttccgcttag cgaggcggct gaagtatgga aaaaaccgct tgaaaaggta ttcccgacaa
      961 aggcatgtca ctttacaagt gagcctgaaa cattcagcta tgataagaga aatataactg
     1021 ttgcaacaga aaaatttgca aaaccgagag tatttattcc tgtattcccg ggtacaaact
     1081 gcgaatacga tactgcaaga gcatttgaaa acgcaggtgc tgtggcagat gtatttgttg
     1141 taagaaacct tactggtgac gacgttgttt actcaatgaa agaaatgcaa aagagaataa
     1201 acaattctca aatcgtaatg attccgggcg gtttctcagg cggtgacgaa cctgagggca
     1261 gcggtaaatt tatcgcaacc gcattcagaa atccgcttgt taaagacgcg gttatgaata
     1321 tgcttaaaaa ccgtgacggt ctaatgcttg gtatctgtaa cggtttccag gcgcttataa
     1381 agcttggtct tgtaccttac ggagaaattc gtgaccttga cagtaattca ccgacactta
     1441 catttaacac aatcggcaga cacgtttctt gtatggcaag aacaagaatt gcgtcaaaca
     1501 agtcaccatg gcttgcaaat gtgaatacag gcgatgttca tacaatcgca ctttcacacg
     1561 gtgaaggtag atttatcgcg tcggaagaac aggttaagga acttgcggca aacggacaga
     1621 ttgcaactca atatgtaaac cttgaagata aggcaacata tgatattgac tggaatccaa
     1681 acggttcggt atatgctatt gaaggtataa caagtcctga cggcagagtg cttggtaaaa
     1741 tgggacacag tgaacgtatc ggtaagaacg tatcgtttaa cgttccgggt aacaaagacc
     1801 aaaagctgtt tgaggcaggc gttaaatatt ttaaataata caaaaaggct gtcggcaaat
     1861 gtcggcagcc tgttttatca cgaggagaaa gatgttatat gaaaccgaaa attggtgttg
     1921 tgtttccaaa gataacggat aaagatatag ccgatttgga aatagaaaaa ataagagttt
     1981 caaaaaacag aagaaaaata ggtattttgc ttggctctga cacgtccgct tatcagcagg
     2041 cgaaggtcga aagtgaagtg aaaaaggctt gtaatcttac ggaagtaatc gttagggttg
     2101 ataacaaaat taaacagcaa gataaaaatg taatgattta cgaagtcgaa cccggtcaag
     2161 agggctctat gcagtctgca tcatcaggcc cgaaaatcaa aggtaaggca aatgtttcgg
     2221 atatgcttta cggcagaccg attaatgacg atattatatc aattcattcg attaacgat
//
LOCUS       DAWACS010000221         2275 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4858_length_2275_cov_2.629279, whole genome shotgun sequence.
ACCESSION   DAWACS010000221 DAWACS010000000
VERSION     DAWACS010000221.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4858_length_2275_cov_2.629279"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          732 a          549 c          356 g          638 t
ORIGIN      
        1 caccaaattc aaaataatcg aaaccaagaa cacaaccgca acgcagtttt cagcgaacac
       61 gttttgaatg atttgcggaa agattgaaaa aatttcggga acttgtgtaa agccaagtcc
      121 gatactcaat gacaatgcgg cgataataat atttctttgt gaaaaaccgc attttgcaat
      181 catctgcaaa ccgcttataa caatattacc gaacatcata agcgtacagc cgccaagcac
      241 agcctcaggc aatgttgcaa gtaatgttcc gaacataggg aacaatcctg ccaaaaccat
      301 aataacgcag cctgtgaaaa tcgtaaatct gtttacgact tttgtcattg aaacaagtcc
      361 cacgttttgg ctgaacgatg taatcggcaa acaaccgaaa agtcccgaca atgcactgac
      421 aaagccgtct gcggcgattg agcctgtaag ctccttcttt gaaacctcac gatttaatcc
      481 cgatgacgca agtgccgatg tatctcctat tgtttccgtt gcggacacaa ggaaaatcac
      541 aagcacggaa ataatcgcgc ctatattgaa tgtcggttta atcggaagta tctgcggaag
      601 tgatacgatt tgtgccgtac tcaatcccga aaaatcaacc atacccatac acacagccac
      661 aatgtaacct acgataagtc cgaacaatac ggacaactgt ttccaatatg atttagcgaa
      721 aatattaaat ccgatacaac aaaccaacgt aattgttccg agaattaaat ttttagccga
      781 gccgaagtcc tccgcacccg agccgccgcc gaacgatgtt gcgccgaccg acaaaagcga
      841 aaagccgata gccgttacaa ccgaagccgc cacaatcggc gaaatcagtt ttacccaata
      901 ttttgcgaaa agtccgagta tgccttcaat aattccgcct ataataaccg ctccgacaat
      961 ggctccgtat ccgtatgtag gaccgacaaa acagaacaca gatacaaacg taaaacttat
     1021 tcccattaca atcggcaatc ccgaaccgac tttccatata ggatacaact gaattacaga
     1081 acctattccc gcaacaatca tagctgtttg aataagtctt gcggtttcgg ttgtgtctaa
     1141 accgcaagca cttgaaacaa taataatcgg tgcgatattc gcaacgaaca tagccaaaat
     1201 atgttgcaga ccaaacggaa tagcttttga aacaggcact tttccgtcta actgataaat
     1261 gttattaata tcactgtttt tcattttatt gttcctctct gaatttaatc atacctgttt
     1321 tgctgtccat atcgtcaact attgccaaag attctaattg aaaacctaag ttacgactaa
     1381 ttcttccgcc tgcttggaag cctttttcaa cagctattcc tattccctca accgttgcac
     1441 cggctgactt gactatctga attaatccct gcagtgcaca accgtttgcc aagaaatcat
     1501 cgataatcaa tacacgatcg ttttcattca aaaacttttt cgcaacaata acctgatttt
     1561 tgcatttgtg cgtgaatgac tcaacctccg caacgtacat ttcaccctca agatttacgc
     1621 tctttgattt tttcgcaaaa acaacaggca caccgaaatg ttgtgcggcg atacaggcaa
     1681 tacctatacc cgatgcctcg atagtcaaaa ttttatttat attcgcaccc tcaaagcgtt
     1741 ttttccattc cttgcccatc tcattaaaca aatcaatgtc catttgatga tttaaaaagc
     1801 tgtccacttt aagcacgtta ccctctttaa ctattccgtc ctttaaaatt ctttcctcta
     1861 aacagttcat ctttactctc ctcaaataat aaaatatata cgattatacc acacctacac
     1921 ccataataca attaatatcg acatcaaatt gcaaaaaaat atgaggactt tttaagtccc
     1981 catatttgtc ttgttttcat ttttcaaatc actgtgaaca cgatgatatt ccggactttc
     2041 catcatcatt acctgatgaa ctccgatacc tttcagaagc ggtaatttaa gtctgtactt
     2101 aacccaaatg aatgcgtata taaacaatgc cgcaaacaga acaaagcaca aaatatatat
     2161 atttcttctt tgtacagggt cagttgatat acttgtcttt tacgtccgtg tcaaagccga
     2221 taaaatatga ctgttctttt cttatctttc ggtcatcgtc taaaatgata aacca
//
LOCUS       DAWACS010000222         2273 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4861_length_2273_cov_1.669071, whole genome shotgun sequence.
ACCESSION   DAWACS010000222 DAWACS010000000
VERSION     DAWACS010000222.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4861_length_2273_cov_1.669071"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    821..830
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1212..1221
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1974..1983
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          719 a          390 c          490 g          644 t
ORIGIN      
        1 gatgaggata acgaggatag aattgacgta ccacagttga caatcagcgg taaactgatt
       61 gacctatcga agttatccga atacggcgac gcagaaaagt tggaaatagg cgacacggtc
      121 cacgtatttg acatagacgg tacggaatat gtgcagaggg taattgagta tcaggcatat
      181 ccgttggagc cgaaagagag caatatatca atcgggcata tcagacgtga tttttttatc
      241 gaactatggc agacgtcaga aaaagcaaag aaatttgcaa agtggcagac ggcgaataac
      301 agcgtaaaca ttcgcaaagt acaaggaacg gtgaacacag accgaaacga agtgcagagc
      361 gacaatgagc tgttgaagat tgtcggcgat ttgctgacga taaaggacag tcaaagagat
      421 agaatacata tcggtaatga tgaagttgac aataaaaaac aatttgtatt tctgttatat
      481 gacgttgacg gaaaccctgt aatatttttt gatgaaaagg gtaacggaat tttcagcggt
      541 acaataagag gtgcaaagat tgaatcagat accgacatca atgtaaataa agacgcaagt
      601 gtaggacagt atttaagagt tggatatatc agctcatatg tgaacgacga gggcaagacg
      661 atatataaat ggtctgatga aagcggtata ttattaagcg gatatacaag cattaagact
      721 acaaacggcg gtaataacct tgcaatcggg gcaatgtcat caatagaatt aaatgcgaca
      781 aaggttatgc agaacggcaa ccgtctattg aataccgatg nnnnnnnnnn atttaaaaga
      841 tattatggaa gaaatcagac aactaaaggc aaaaatatca gagttggaaa attaaaaaaa
      901 caaagacctg tacacaagaa tagtacaggt ctttgtatta tctcaaacta tttatagtgg
      961 gtataacagt tgaaacataa taatcatatg gaattaggga gttagtgtct tcggggtgcg
     1021 gtacattttc aagaatggct ttgccgtcat cacgggtgct atcataaaat acaacagatg
     1081 taaatttgta ttgacctaac ccgatatctt caagcatttc ttcaatattt tctttgcgaa
     1141 catactgcac accgtcaatt acttcgattg ccacttcggg taacggcgat aagtcggaag
     1201 tggttgacgg cnnnnnnnnn ngtaatactc tttgtttgga tttaatgtag gctttggttg
     1261 tgtagcggtg gttgttgtag ttggtgttgg ttcggcggcg gtatctgtat caatcgtaat
     1321 agtgttgtca ctgaaaccaa cattgaaacc gccgacagcg tcggcaacgt cacgtaattt
     1381 gaaatatgta ttatcgttga tgttataacc ctcaatcgct gtttccgtac cgtttacggc
     1441 aacaggaaac gggttagccg ttacgacata ttctacggcg aaacctgtcg cggtcgcaca
     1501 gattataccg cctgttataa aacctaatat aaatttgttt ttcatagctt gtagcctcct
     1561 tttttctttc agtatatacc cttaaaatga aaaatgcaag cggcggaaaa ttgatttttg
     1621 ttaagttttg ttaaggtgtt cgcgcacgca cacgcgcgct ataattgaaa gaaatttaaa
     1681 aacagcaaaa atatattaac aactgttata tcaaacgctt accgaatggt aggcacttta
     1741 tttcgttaag ctgtatgcgt gcgagtgttc caacgcttta taacatccga tagaacatca
     1801 agccaatcgt gaaatttttt tgttatcata tcttgaccgg taacggctat aacagtacaa
     1861 gagcggcaat gttcgcactg tacttttaca caagggctac cgtatgaact aagcggaatt
     1921 aaacgagcct tgccgccgca aaaaggacaa gcgtgtaatt ttttgtgctt gaannnnnnn
     1981 nnnagcttat acaaggcttt tcttttatga taattgcaag gttattaccg ccccgtaatt
     2041 cataaaacgg gtgtaatgcc ctctaacctc tttaaaggtg gttttaagct taattccttt
     2101 atcggctatt gctgatatga cataagctaa attctttgga atatagccta ttttgtacgg
     2161 catagaattg tttacactta tcataaccgc tactgcgttg ttatcgtgta aattcgcctt
     2221 gtcacgttca agccatactc ttacttgctt tggtttgtag tgtgtgaata ttc
//
LOCUS       DAWACS010000223         2271 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4865_length_2271_cov_2.739170, whole genome shotgun sequence.
ACCESSION   DAWACS010000223 DAWACS010000000
VERSION     DAWACS010000223.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4865_length_2271_cov_2.739170"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          573 a          601 c          359 g          738 t
ORIGIN      
        1 attataacag ataaatcact ttacttcaac tatttttatg ttttgtgctg aaattttaag
       61 ttgttcggtt gcgattgacg taagttttac tacatcttcg tctgaaaatc cgtcttttcg
      121 caccataata tttgcctcac tgccgtctac atacacacat atttcactgt agccctttga
      181 ctgtgcgata ctttcgattt ccgattcttt ctgtacgttg tttgcctctt caagaatttt
      241 gtcaccggct tttttacgaa tatcggcgtc aaagctttcg ttttcggcag tttgatttaa
      301 tatttcaagt gactttgaac gtgatgtttc tttattcatc tttgcctgtt caaaataatt
      361 gcccgaatct gacgcggttt ctaccgttac attgctttcc tgactgtccg ttgcctgttc
      421 gccgttatct tccgttacag ccgtttcctc cgcttgctcg ctgtcgtttt gttccgcgtt
      481 gtccgttgat gtttcctccg ctaccgtatc ggaagaaaca tactgtgcct caccgagttt
      541 cttgccggtt tcaacgtagc tttcgccgtc ctgtaccctt attgtatctt gatacgacca
      601 attcaagtat ccggcaactc ctataagtac cacaagcgat gccgctatta tctccttctt
      661 cttcaaaacc attttatttt acctccttct taaatataca tattctgtgt gcaggcacgt
      721 caagtgatgc tgtaaccgcc tcggaaacag cacttctgac tgcgctgttc ccgcccccgt
      781 cggcagtcac gataacacct ctgacttttg gatacacctc ttttaccacc ataggttcac
      841 cgtctgtcat aacagccttt ttatcctcgc tttcttcctt ttctcttgat gaaactttcg
      901 tttcgtaagc aatattcttt tccgaacttg aataataggt tatcataaca gatacctctc
      961 cggcaccgtc aatctgtgaa agaatgttcg caagcctctc ttcttcatct tcaacacttg
     1021 ttacggctgc cgtaggttgt acgtcagctt ttttattttt gtccacactt gccactgcca
     1081 taagtacaac tccaattatt aatattaagc atatcagtct gttattctta tttttaaata
     1141 ttttaagtaa atcttcctta ttcatcatgc acctcataag gtttcaaacc gtatacctcg
     1201 catattctgc tccttgcacg gtccgttaat ttatccccgt aaacgcgtac actgtctacc
     1261 cctgtaatct caccttcgtc attcaccatt atttcacatt ccgccgtaac cgataattta
     1321 aactcttttt ccattcgtgc cttaacatcc tcgtttatcc tgtttttcag ttcttctata
     1381 acaagttccg tctgcatatt actgctgtcg gctatgtttt cctgtacact gtcaaagccg
     1441 tcgaatatat cactctttac gatagccgtc acaggagaaa tgatacagct tattataacc
     1501 aaacctgtta taacctgtac ataactgcgc cacttttcag gtgcaataat cccagccata
     1561 gctgaaagca aagtcgcacc gattatggtt atgatatatt ctttcatcgc ttatcctcct
     1621 atgccagtgg cggctaacat aatacttata ttaatcataa aaagtaccgc taccataacc
     1681 actaccgcaa atatcgttgt aactgactcc gacacctccc aaagcataga cgaaacacgc
     1741 ttatccgtaa gcggttcggc tattgatgat gtgagtttta gcattaactg tatcacgccg
     1801 attttaagta tcggcaatac gcatataacg cacatcacaa taattcccga tgttccgacc
     1861 gcatttttca taagccttgt tccgctcaca accgtttcaa gcgtatcgga taaaaacgta
     1921 ccgacaacag gtacaagact tcctaccgca aacttaaccg ccttaccgct taccgcgtca
     1981 agtgcgggag aagaaaagcc gtatatcgca cttataccgg taaaaatcgt tatgatcgcc
     2041 gccataagcc actttgaaat cgacttaacc accttacaaa atcccgaaag ctgtaccttg
     2101 tcactgatat tccctgcaac cgcaagcacc gagctgaacg ctataagcgg tacaaggcac
     2161 ttctcaataa ttatcgacac aacatacacc gccgccgaca taacaggctg aaaagttgac
     2221 gccgatacca ccttaccgct tgtcgcaatc ataatcatca acagcggtga a
//
LOCUS       DAWACS010000224         2270 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4875_length_2270_cov_2.319187, whole genome shotgun sequence.
ACCESSION   DAWACS010000224 DAWACS010000000
VERSION     DAWACS010000224.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4875_length_2270_cov_2.319187"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          773 a          375 c          546 g          576 t
ORIGIN      
        1 aagagggcag agccgttatt cagacgtata cgcccgaaca tgaggcggtg agccttgtaa
       61 aaagtcacaa ttacagaact ttttacgata aggaaattgc aaaacgaaag cttatgtggt
      121 atccgccgtt ttgtaaaata gtgagtgtgc attttcaagg caatgttgaa aatactgttg
      181 cgaaaacggc aaaatatttt ttaaacacta cagaaaaaac acagaatgta tcacaaaaaa
      241 tacaggtttt aggacctgtg ccgtcatata tttcaaaaat aaaaaacaaa tacagatggc
      301 agattatttt taaatgcgaa gatgacgata acttgggtga gatactttca caagctgaaa
      361 tgacatgcag aaaaaacaag gaattttcgg atgtggcgat aataatagac aaatcacccg
      421 gtatgataaa ttagagaggg gaaacataat ggcaatcaga gaaataaggg aaaaagacga
      481 tgaaatcctt tataagaaat gcaaagctgt agtgaagttt gacgaaaaac ttcatatact
      541 tcttgacgat atgtatgaaa caatgcaaag ccgtgatggt gtcggacttg cagctccgca
      601 agtgggaata ctgaaacgtg cggttgtcat agatgtcggt gacggtaaaa ttgaacttat
      661 aaaccctgaa atagtagaag aaagcggaga acagaccggc agcgaaggct gtcttagcgt
      721 acccggagtg ttcggcgagg ttacaagacc gaatgtcgta accgtaaaag cacaggacag
      781 ggacggcaag tggtttaaaa tcacaggtaa ggaactcttg gcaagagctt tttgtcacga
      841 aattgaacat cttgacggta aattgttctt ggacagagta attagattta ttgactaacg
      901 gagatggttg aatgagaatt ttatttatgg gtacacccga ttttgcggcg gcgagcctta
      961 aagctatgac agacgcaggt cttgatgttg ttggtgttgt atctcagccg gacagaccga
     1021 aaggcagagg acataaactt gtaccgactg atgttaaggc ggcggcactt gaggcaggta
     1081 tagaaaatat atatcagccc gaaaaactga gaaacggtga acttcagcct gttcttgaca
     1141 aactgaaacc ggagcttatt gtcgttgtcg catacggcaa aattttgcct gattatataa
     1201 tagattatcc gaaatacggc tgtgttaatg tacacgcgtc acttttgccg aagtacagag
     1261 gtgcgggacc tattcagtgg gcgattataa acggcgaaaa ggttaccggt gttacgacga
     1321 tgaaaatgga cagcggactt gatacgggcg atatgctttt aaaagcggaa accgaaatcg
     1381 gtgaatatga aacggcagag gaactttttg accgtttagc cgtaataggc ggcgatttgc
     1441 ttttaaaaac aatagacgga cttgaaaaag gcagtataac acctactccg caaaatgata
     1501 aggagcatac atatgcgcct atgatttcaa gagaaacggg tgttatagat tggagtaaat
     1561 cggctcgtga aatttcaaaa ctaatatgtg gcatgaattc gtggccgctt gcttgcacaa
     1621 aatataaaga cggaatttta aaaattgttt ccgcaagagt ttgtgatgaa aattcggata
     1681 aagaggcggg agaaatactt gcacttgaaa agggaaaagg acttaaaatt tcttgcggtg
     1741 agggaacatt gtacattgta acggcacagt tcccaaacag taaaaaaatg aatgttgaag
     1801 attatgcaag aggacatgaa attgaattag gagttgtact cggaaaggag taattatgtt
     1861 cagctattat tttgacccaa cgtatgtact tgtaataatt gcatttttac ttacattgtt
     1921 tgcgtcaatg ggagttaaat cgactttcag taaatacaat gatgtaagaa gttcaagagg
     1981 aattaccgcg gcgagtgcgg caaggcaaat tcttgatgca aacggacttc agaatataag
     2041 aattgaacac gtttcgggtg accttacaga ccactacagc ccgaatgaaa acgtcataag
     2101 actgtcggat tcaacatata attctacatc ggtagccgca ataggtgttg cggcacatga
     2161 gtgcggtcat gcggttcagc accaagtagg atacgttccg ataaaaattc gtaacggaat
     2221 tgtaccgata gtaaatgtct gcaacacgct ttcaatgccg attttcataa
//
LOCUS       DAWACS010000225         2260 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4904_length_2260_cov_1.540590, whole genome shotgun sequence.
ACCESSION   DAWACS010000225 DAWACS010000000
VERSION     DAWACS010000225.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4904_length_2260_cov_1.540590"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1242..1251
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          781 a          357 c          482 g          630 t
ORIGIN      
        1 gcaatagttg ttgtaagctt aatgaacatt gcttccttag tagttccgcc gttcgtttta
       61 aagaatgaat cgcccatatc agaagatgca ccttgaatac ctctcatacc cggatcctta
      121 ccttcctgag ccataactac aaatataagt ataacagata ctatcaaatg taataccaaa
      181 aatagagttt gcattatata aacctccttt ttcttctaaa gtaaatcatt aaatacttat
      241 ctattatatc acaaattcta aaatttgcaa tacttttttt gcctttttgt aaatcagttt
      301 tgtttttaat gcttttgagc aaaatatgat aaaaaatttc tgataatagc aaaaaactga
      361 tggtaactgt acttaaattg atgtataata ttatcataat taatattacg gaggaatttt
      421 gtaatggaaa gattaaagag aacggaaaaa aacacactta cggaacgtgt acttcaattc
      481 ggtgagggca atttcctgag aggatttgtg gattggatga tagacaaact taacaaggaa
      541 aacggcggag attacggtgt gacaatagtt cagccgctta cgggcggact tgttgataag
      601 ttaaatgcgc aggacggacg atacagcttg tatttaagag gacttttaaa aggcgaaaag
      661 gttgaggaaa caagaattgt tgattgtgtg acaagaggta tcaatccgta tacaaataca
      721 gatgaatttt ttgactgtgc aaaaaatccc gacttacgtt ttatcgtttc aaacactaca
      781 gaggcaggta tcgagtacaa acctaatcaa aatcccgatg attttaacgg acttactttt
      841 ccgggcagac ttacgttgtt tatgaagaaa agatttgacg agggactggg cggttttata
      901 cttcttccgt gtgagttgat tgacaaaaac ggtgacaatc taaaggaatg tattttaaag
      961 tatagcgagg attggggtta cggcggtgaa tttgagaagt ggattgaaac tgaaaaccat
     1021 tttacaaata cacttgtgga cagaatagtt acaggttatc cgcgagataa cgcaaaggaa
     1081 atggagcagt catacggtta tcttgatgat atgattgaca ctgcggagat ttttcatttg
     1141 tgggtaatag agggcgataa gaaacttgcg gaggaaatac cgttccacaa aatcggtctt
     1201 aatgtgcttt ggacagacga tgttacacca tacaagaaac gnnnnnnnnn nggcacatct
     1261 tgccggtctt gaaacagtca aggacgcaat ggacgatgaa cttgtatttt catttatgaa
     1321 acagggtatt tttgaagaaa ttattccgac acttgatttg cctaaaaacg aacttgaaca
     1381 gttcgcaaat gacgtaatcg aaagatttaa aaatccgttc atcaagcatt atcttttgag
     1441 tattgcgctt aattccgttt caaaatacaa agtgcgtgta cttccgagcg tgcttgaata
     1501 tattaaggaa aagaattgtg agccgaaacg acttgtattt tcacttgcgg cacttatcgc
     1561 attttacaga acagacgcgg cgaatgatga taagacggta atggaattta tgaaaacggc
     1621 aagtgcggaa gatattctta aaaagacgga atattggggt gaagatttgt cattcttact
     1681 tccgaatgta caaaaacatc ttgacgaaat cgaaaagaac ggtataagaa aggctatgga
     1741 aaaggtagtt gactgaaaat gaaacagata aaaattaatg aaaaggataa cgtagccgtt
     1801 atgcttgagg gcgaattgag cggtcataag attgccctta acgatataaa aaaaggcgaa
     1861 aatataatta aatacggtta tcctatcggt catgcgacag aggacataaa aaaaggcgac
     1921 catatacata cgcataacat taaaacgaat ttaagcggtg tgcttgaata caaatacgaa
     1981 ccacaaagaa acgaaatacc gaaaacggaa aaacgcactt ttatgggtta cagacgtgca
     2041 gacggcaaag tcggtataag aaatgaaatt tggattgtga acactgtcgg ctgcgtgaac
     2101 aagacgtccg aaatacttgc aagagaggct gataagctgt acggcgatat gtgtgacggt
     2161 gtgtttaatt atgtacatcc gttcggttgt tctcagcttg gcgatgacca gaaaatgact
     2221 caaaacgtgc ttaaaggtct tgtcaatcac cctaatgcga
//
LOCUS       DAWACS010000226         2224 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4978_length_2224_cov_2.498847, whole genome shotgun sequence.
ACCESSION   DAWACS010000226 DAWACS010000000
VERSION     DAWACS010000226.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4978_length_2224_cov_2.498847"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          715 a          422 c          548 g          539 t
ORIGIN      
        1 ccttaaagga acagcttgca aaagcaggtg tgacagatgc agattatctt atttacaagc
       61 aaggcggaat tgacaagttt acattcgaca aagacggcaa gcctgtcggt gtggacgata
      121 ttcttaaacc gcttagggag gataagacgt actcacacct ttttgccgaa aaaggaggag
      181 catatacacc aaaaggcgga agcggaagtt cagacgtaaa tccttgggca aaggaaacat
      241 tcaatcttac caaacaggga gaaatttata aaaacgatcc tgctaaagca aaagtattaa
      301 tgcaagaggc aggaataaca ggaggaattt aatatgggaa caactttatc agatattatc
      361 gtaccggaac tgtttaatcc gtacgttatt caaaaaacac ttgaaaaatc ggcacttgtg
      421 cagagcggta tagttcaaaa cgacgcagag tttgacaagc ttgcgtcaca ggcaagtccg
      481 cttgtaaata tgccgttttt ctctgaccta acaggtgaat cggaaacggt tatcgagggt
      541 gacgacctca ccgccgacaa aatcagcagt aagaaagacg ttgcggtaat tttaagacgt
      601 gcaaaaatgt ggagtgcgac agacctttcc gccgcaatgt cgggtgctga ccctatggcg
      661 gcgattgcaa gtcttgtatc tgacttttgg gtaagagatt tacaaaagga gcttatcgca
      721 gtgctgaaag gtatcttcgg cacaattccg gcagtctccg acggttcgcc taaagaggct
      781 gaaacaagac ttgcgtcaaa cattcttgat atgtcaagcg caagcggtaa cggtgcaaaa
      841 tggagcggaa gtgcttttat tgacgcacaa cagcttttag gcgacaacaa agcggaactt
      901 accgccgttg ttatgcacag tgcagttgag gcggcactca gaaagcaaga ccttattgac
      961 gtaattcagc cgtcgggggc aaatccgttc agtacatata tgggtaagag agttattatt
     1021 gacgacggct gtcctgtaac aggttctggt tcaagtcaag tattttcaac gtaccttttc
     1081 ggcaacggtg cgattgcact cggtaacggt acaccggaaa agtttgttgc aacagaaaca
     1141 gacagagata agaaaaaggg tagcggtgtt gattatctta tcaatcgtaa gacgtatatt
     1201 cttcacccac gcggtgttaa gtttacggac gccgatgtcg caaatacgga aggtcctacg
     1261 cgtacggaac ttgccaacgc aaaaaactgg acacccgtat atgaccctaa gcagattaga
     1321 attgtcgaaa tgcgtcacaa gatttgatga ggtgacttat ggacgagtat ataactgttt
     1381 ttacggatat gtacggcata agcgaagatg acagaggaaa agccgaaagg tgtattgaaa
     1441 gcacaatcga atatatcaag aattattgcc acattgacag tattcccgat gatttaaagc
     1501 ataccgttat acttatggcg gcggacttgt tccgctatga cgtgtcgtca tcatcggggc
     1561 aatatgacaa tgtaacgtca atcaaagagg gcgatgttac cgtatcgtac ggcagtaatt
     1621 caagcagtat gtcgagcgtg tttaaagact acaaagcaag gcttgcacgt ttcagaaagt
     1681 tggtgtggtg atgaatatgg taagagaggc gattgaaaga ctgtataaag gcttatgttc
     1741 tgtcaaagtg aaagtttcaa gcgtgaatga ggaaacaggc gaaactgtat ttaccgaaaa
     1801 ggctgtttta acggaacagc cttgccgact ttcgtttcaa agccgaaatt cggcggcaaa
     1861 ggacgacgga tataacaccg tatcgcaatc ggttgtgctt tttattgcgc cggaggttga
     1921 aataccgtcg ggcagtaaaa taaccgttac acaaaacgga aaaacaactg actattgccg
     1981 tagcggtgaa agtgcggttt atacatcaca ccaagaaatt gcactggaat tattcgagga
     2041 ttatgcgtaa tgaatgagat tgatttttca cagcttgaga aattacaaaa gcaaatggaa
     2101 agtgcggatt acaccaaagc ttgtgtatcc gctatgaatg agatttctca aagagcactt
     2161 aaatatataa gtaacgtaac aaagccaggg cattacaaaa acggtaaagt gggcggtaca
     2221 ctga
//
LOCUS       DAWACS010000227         2218 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_4997_length_2218_cov_1.885807, whole genome shotgun sequence.
ACCESSION   DAWACS010000227 DAWACS010000000
VERSION     DAWACS010000227.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4997_length_2218_cov_1.885807"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1972..1981
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          678 a          390 c          461 g          679 t
ORIGIN      
        1 ggtgtttata ccgtttacgg taggcggcgg aatacgaaca gttgaagatt tcagaaaaat
       61 tcttaatgcc ggtgcggata aggttgccgt caacagtgcg gcgataaaac gtcccgagct
      121 tatcagcgag gcggctgaaa aattcggcag acagtgcgtt gtctgtgcga ttgacgcaaa
      181 aagacgtgaa gataagagcg gttgggatgt gtaccttaac ggcggccgtg tgaataccgg
      241 aattgacgcg cttgaatggg cgaaaaaagc cgaggagctt ggtgcaggcg agatattgct
      301 tacaagtatg gattgtgacg gtacgaaagc cggatacgat attgaactta caagacgtgt
      361 ttcggaaaat gcgaaaatac ctgttatagc gtctggcggt gcaggcaaga tggaacattt
      421 tcttgacgca tttacagagg gaaaggcgga cgcggttttg gcggcgagtc tgttccattt
      481 cagagaaatt gaaattaacg atttaaaaaa atatctgcgt gaaaacggtg aaagcgtcag
      541 attaaaggag gaatttatga gcaagaacag aagaaagtca ctgaaaaaag aacctgtaat
      601 tccaaaaacg gatttttcgt tttacgaatc gaaaatatat ataattgcga caattattat
      661 gtttcatatt gtaccgcttg tatttgttat gatgggcgaa aacggtcaat tattactgtt
      721 acaatttttc cttatgatgt taaacccaat gtttatcgca ttatctggac ttatatacgg
      781 aataaaacag ggatttaact ttaaatttcc gctgtttatg gcgattatct caatggtatc
      841 aataccgatg tactatcagt ttgacgcggc ggcaaatatg atgatgacaa cgattattat
      901 gtgtatagtt tatgcgatat tctcatttgc ggcaacagta atcggagcat ttgtaaaacg
      961 acttttacgt ctgtaatcac agaaaggata tatatttatg gagagtaaaa aactttatgg
     1021 tgcatcaacc caaatgacgc ctgctgttta taaagatttt tataaaacat attataacga
     1081 aaaactaaag gtgtttaaaa tcgttgccga aatagtcgga ttgattgcga tttttggtgc
     1141 attttatata cttgcaaacg gatatggaat tttgtgggga gcgattgcac tttggattgg
     1201 tgcatttctg cttttttatc cgcgtatgat ataccgcaag ccatacaaaa acgcaaaaga
     1261 caacagtcag acaacgcact ttgctttttt tgaaaattat atgtcggaaa aaacaaaatc
     1321 acacgtaaat gaatataaat atgacgatat acaaaaagtg cttgaaacgg gaaagtattt
     1381 tttcatattt cacaattcgg aaagtgtgag tatcgttgat aaggctcatt ttagcggaga
     1441 tgtcgaaagt ttttcgggtt ttataaaaac aaagacacaa tataagaaaa tcaagtaata
     1501 aaaaatggga cttagcaaaa atgccaagtt ccatttcttt ttatcgctac aacagttctt
     1561 cccaccccgt aatataatgg tcagacagtt gtttaagcgc atctgtttca tcggcgtttg
     1621 tttcgtcaaa tattgcacag gttgagaaac cacccttttt agcaccgccg atacctgttg
     1681 ttatatcttc aaatactgta cattcttccg gaggtattcc gatacgtttt gcggcaagca
     1741 aataaacatc gggatttgat ttgttcactc cgacctcgga cgagaatgca caagcgtcaa
     1801 tgtaattcca aactccgagc ctggtaaaag ccgttttgca aagttcttca taacctgatg
     1861 tggctattgc gattttaatt ccgctgttgt gcaatttctt taaatatttg tctgcacctt
     1921 tttttagcgg tacggttgtt tcgtattgct ttaatcccat gtgcataaaa gnnnnnnnnn
     1981 ntgttgggat agattctttc atagtcattt ctttgtattc ctttgcctcc tcgtaaggca
     2041 attcaacacc gtgttctttg aaaaaatcta ctacaacgtc ccaccaaacc tgcattgaat
     2101 caagcaatgt tccgtcaacg tcaaaaatcg ctcctcgcat tatatcacct ctgtatttct
     2161 attatacaat aatttgccga atatttcaag cgtttatgaa ttttcctctt tctcggca
//
LOCUS       DAWACS010000228         2204 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5042_length_2204_cov_2.287110, whole genome shotgun sequence.
ACCESSION   DAWACS010000228 DAWACS010000000
VERSION     DAWACS010000228.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5042_length_2204_cov_2.287110"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1099..1108
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1593..1602
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          717 a          335 c          518 g          614 t
ORIGIN      
        1 cgcgttatgc ggcgatattt acttacagca taaaagacga tattgtacgc gtaaaccgtg
       61 caatcggtca cagaaacaaa gaagttgaat tttatatatc ggacgtgaag tctgttacca
      121 gacaaaaacc gaaaaaaact ccgaaaacga tatacaatat gcgtgcgagt gtattttcgc
      181 ctaaaaaggt ttggtatgtt gtttacgaaa agaacagaat agaaaatatg cttgtttttg
      241 agccgtcgca gaaaatggct gaaaagatta agagcagagc gaaaaacagc aaagcttaaa
      301 cattttggaa ggaatggttt tataatatgg taaacgtgat aggggtacga tttaagccgg
      361 caggcaaaac gtactatttt gatccgcttg attttgatat aaaacaaggc gacagtgtaa
      421 ttgtcgaaac ttcaagaggt attgaatacg gtgaggtcgt tatgagcaaa aaggctattg
      481 ataacgacca attcaagaaa cctttaaagg gtgttatccg tatcgcaact gaagaagata
      541 ccgaaaaaag caaaaggaac agagagcttg aaaaagaggc ttacaagata tgtttggaga
      601 aaattcaaaa tcacaagctt gaaatgaagc ttgtcgaagt tgaatacaca tttgacggaa
      661 acaagatttt attctatttt accgcggacg gaagaattga cttccgtgaa cttgtaaaag
      721 acttggctat ggtctttaag acaagaattg aacttcgtca aatcggcgtc agagatgagg
      781 cgaaaactct cggaagtatc ggtgtatgcg gcagaggttt atgttgcagt cagttcctcg
      841 gtgagttcgc tccggtgtct ataaaaatgg caaaggaaca gggactttca cttaatccga
      901 ctaaaatttc gggtgcttgc ggacgtctta tgtgttgttt gaaatatgaa caggatacat
      961 atgaggaact tttaaaggtt acaccgaaac agggtgctat cgtaaaaact cccgaaggta
     1021 cgggcagtgt tgaatatgtc agcttactta aaggtttggt taaagttaaa cttgacgatg
     1081 aaaacgaaaa aactttaann nnnnnnnnct tcagaggaag aaatcggaga gtaaaatatt
     1141 ttgaaaaact attgaagtat atcaattttc gtgttataat catatcagca atatcaaatg
     1201 aggaggtgta tttcaatggc atataagatt tctgatgctt gcatttcatg cggtgcttgc
     1261 gctgctgaat gtcctgtaag tgcaatcagc gagggtgacg ctcaatacgt tatagatgac
     1321 gctacttgta tcgaatgtgg tgcatgtgcc ggtgtctgtc cggtaggcgc tcctgcacaa
     1381 gcgtaattaa aaatgaactg agaaggtgtt gtataagaga aatctttgca acaccttttt
     1441 acattatata aatttatgga ggggtaaggc tatgaaaaga gttacgctta taataactgc
     1501 cgttttgacg gttcttatgc ttacggcgtg ttcttcaaat aaggtggttt atgaaagtaa
     1561 taccgaaagt caagggggta acaaagcttg gannnnnnnn nngttcttat atatatgtgc
     1621 ggcggtgacg atgaatccgt aaacggtgat tattcaaaaa aacttgatga aattatgaat
     1681 gtaaattatt cggacaatat aaatgtcatt gtgcaaacag gcggcagcag taagtggcac
     1741 actaagaata tatacagcga ttattgtcag agatttaaag ccggtaagaa taaactttat
     1801 cttgaggatc agtctatggc ggcaaatatg ggtgactata atactcttgc taattttata
     1861 tcgtggggca cgtcaaagta taaagcaaac cattatatgc ttattttatc aggtgcaggc
     1921 ggcggattga taaacggtat ggcttatgac gagcttaacg gcaacgacag ccttaatctt
     1981 gaagaaattt catacggaat cagtgcggcg ggcgtgaatt ttgatatgct gtctttcgat
     2041 tcgtcactta tgggaagtct tgaaattgcg gcggagatgt ctatgtgtgc cgattatatg
     2101 actgcaccgc aggacgttat cggaaatgat gaatggaatt atgaatatgt tttacagtat
     2161 ttgtccgaca atccgtcaac tgattctcaa ggtataggta aggc
//
LOCUS       DAWACS010000229         2193 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5077_length_2193_cov_2.036015, whole genome shotgun sequence.
ACCESSION   DAWACS010000229 DAWACS010000000
VERSION     DAWACS010000229.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5077_length_2193_cov_2.036015"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          585 a          527 c          327 g          754 t
ORIGIN      
        1 tgatattcag ccacatttac tcaccgccat gctctcaccg tccatagctc gctctgtgtt
       61 ttcaatatga catactgtct tcgtcatcgt ctttattgtt ttattatatt acaaaatatt
      121 tttttagtca acagttttta ttgaaaaatt tcctgtctgc caatgttttt agtcgatatt
      181 atcgaccatt ttcataacat tcgcacaaag tgacttaatc tgcattttat actgttcaat
      241 ctcgcctttc agcgaaagca tttcttgatg gcgtttcata tgttcatcat caatctgacg
      301 tgcaagataa gacgcgtcaa gctttgcttt actgataatt gcattcgcct ctgtttcggc
      361 atttctcttt gcgtcctcgg cggcttgctt ggcaatgtca atcgacttct gcattgtgtt
      421 ttccatttgc ttgtaacgct ccaccgtttc ggtcaaaagt ccgattttat ctttaagcgt
      481 ctgattttct ttacacaaaa gttcgtaatc ctgtattaca acgtcaagaa aatcgtcaac
      541 ctcatcacaa ttatagccct tgagtttctt ttcaaattcc ttattttgaa tatcaatagg
      601 tcttagcaca atgctccccc tccttacacg aacttctcca cagttatgtg aagtcttcct
      661 ttcctggtat tgttcccaat atcctttaaa ataaatcttc cgtaattgcg tatggataat
      721 aagtcaccct caccgacttg ctttgaacgg tcaagcactt cccggtgatt cagttttaca
      781 tatcccgaac ttattaattt ttccgtattt cctcttgata tattgagtgt cgccgcaact
      841 accgcgtcaa gacgcaatga cgcacatacg cacatttttt cggtcgtttt cggtttaggc
      901 ggtataaagt ccgccatatc aacgattttc gtattaacac ccgcatttgc aatcttattc
      961 acgtttcgtg caacatattc cgctatatcc gacattacaa acacatatgc gccctcatca
     1021 tctgtcaaaa tatcgccggt tttactcctg tcgataccaa gcgacataag cgtaccgaga
     1081 taatccctgt gcgtcagttt acgaaacttc ggcacatcaa aacgtataac ggaaatcggc
     1141 acttcgcttt cctcagactc ctgccattcg ggaaatacgc aaagcacctt tctttccgca
     1201 ttttcataac cgccgaaaaa caacgtatta tatccgtacg gaatttgaaa ttcgtcctca
     1261 atcacggcaa gttcaccgcc gtcaaggaaa tcggaacatt tcatttcaca atgcttatcg
     1321 caaagcgaaa acaaatcgtt tatccttgca ataagcaact tatgatttgt attcatatcc
     1381 acctcatata caaaagggtg gtaatatccc ataccaccca gccgtatttt gtttaattat
     1441 aagcaaatcc aaatatcgga ttacataaag ttccaatcca taccgttttt aacgtgttct
     1501 ttcattgtat cgcctgttat atcaatattt ttcggagcgg caacaaaaat accctctgat
     1561 acacgtctga cttttccgtt tacgccgtaa gccgcaccgg acataaaatc aacaattctt
     1621 cttgcttctt cattgtcaat ctgtcctaca tcaaaaataa caagtctgcc ctcttttatt
     1681 tcattggcaa catttgtcga atcgttaata tcggaaggtc tcattatctt tattcttgaa
     1741 gatgatgtcg gattgatagg cactaccttt gagaaagtct ttttcggagc agactgctgc
     1801 gaacttctct tgacacgcgc gttttcttcg tcttctgctt cttcctcatc ataatagtcg
     1861 tcatcataat aatcatcttc actttcaaag cccaaaaagt gctttactgt gtttaaaaaa
     1921 cccatttgtc cgtatccttt cattattact tattttgaga ataatctctt gcaccgaata
     1981 tggcactgcc gacacgaacc atatttgctc cgcattcaat ggcagtttca aaatccccac
     2041 tcatacccat tgataggtat accatattaa cattatcata ttttttttgc tttatgtcaa
     2101 caaaaagttg tcgcatgttg tcaaaatgta atctgtttgt aacagaattg tcagttttcg
     2161 gtgctattgt catcaaacct ttaactttta cgt
//
LOCUS       DAWACS010000230         2191 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5085_length_2191_cov_1.584270, whole genome shotgun sequence.
ACCESSION   DAWACS010000230 DAWACS010000000
VERSION     DAWACS010000230.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5085_length_2191_cov_1.584270"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1477..1486
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          529 a          488 c          391 g          773 t
ORIGIN      
        1 tcgtcctttc cctttattgt aggatacaag cctgccaaat cctccgatac ggttaacttc
       61 gtccacgcac cggccgtaag attttgacct gttgtgtcac cgttccatgt taccgtacag
      121 tttctgtttg cactcacata tacagtgtca ccgaccttta aacgaccgag tttatccggt
      181 gactccttac tgtctgtacc gtcacttgca agactgaatg taagcgacaa atctttataa
      241 atgatgctgt caaccttaac aaccttttcc gtcagattgc ctgcagcgtc cgcaaagtga
      301 tatacatatt cgccgtttgc gtttattact cgtgtaaatt cagttccctg ttttccgttt
      361 tctctgaaaa taacttcttt gtcggctttt aaagtcactg tcactttacg gtgattttcg
      421 gacggctgaa ttgtttcggt aataatcggt gcgtttttgt cgatgttctt aacctcattc
      481 aaagtaattt cacacacttg accgtttacg gctgtacaaa caagcgtcat agtcggtgca
      541 ttttccgaat atgaaattgt aacttgatta tcaagtttat atatttcagg aagataaccg
      601 ctttgatttt tccattccgc tttcgtgatg agcttatcaa atctaagctg tgccgtaacg
      661 gatttattcg tcttactgct ctttgcgttt tcagaccaaa cttcactgat tttatttgca
      721 ggtgaattgt ctatttccgt aacggctgct tttaattcac cgctttcgtt ctcaccttta
      781 tatgtgtatg tgtagtttat aacaatattt tcgttgtctg ttacttctat ataatacaag
      841 ccgttgtatt ctccgtcact tgtttcctgt tttgcgttgt cattagtcat aacaaggttt
      901 gtaaagtcac tgtcctcagg cggtattagg tataccttta cggtctgcat atcgcccgac
      961 acgactttct gtaaatgcgg ctgaggtgct tcgcccaagt tatccgcaac aagcggaatt
     1021 tcggtgacat tattacagct gtcgactgca aacaatgtaa attccgcatt ctttgttata
     1081 tcaacggttc tgccgtttac actcactccg tcaatcgtat cgcttgttga atcaaatgtc
     1141 ggtgtttcgg cattaagtcc cgcttttgca ataagcttta caccgtttat atcagacacg
     1201 attacattaa atctgtaatg tgacgaccaa cccattttct tcataaatga tatactgtcg
     1261 gtaactgttt catagcctcc gccgcttaaa tcaggaagtg acggatttaa gtaatgcttg
     1321 ttatcatcat acagattaag cacaagtttt ttactttgtg ccacgccgcc ccttacggta
     1381 taaccaacca tttcaacact cggcggcatt gtatcgacat attcttcggg cggctgataa
     1441 tctttaagtg ttacatcaag cgtaactgta taatctnnnn nnnnnnaagt atcattttga
     1501 tttttgtaat tcctgaatgt aaattccgtt tcactgttag gatagaatgt atggctcggt
     1561 gtttcgcccg ttgcggtatc aacaagtgtt gagccgtttt catcaacaag ctttgcggtt
     1621 acttcgccgt atatacagcc gtcaaccaca tcatcttcgt cataatccca tacaactttc
     1681 ggctttatcg gttttatctc attattggtt atatttaata ttttactctt ttcagctccg
     1741 ttatccgtcc acttcattgt aacctttgta ggctgtgttg cggttacggt tacgttattt
     1801 gttccgttgt tttcgatttt tgcaaatgtg tttccgtcat tttccgtaac agttacggga
     1861 gaagtatatt cgatattcgc cgtaatatcg ccctttgtct tatacaaagt tgacattgta
     1921 acctttggac cgtctgcaag aagtgtaaca gttacaggta ttgtccatgt attgccgaac
     1981 atatcctcgc cctcaacttc gtatgtaccg tttgagtata tcggcagata gtgagcagac
     2041 gcataaaccg tatcgccctt taattttaaa tatccgttaa agtatacctt aacacctctg
     2101 tcattgcaga tataactgta atccgctaat gaatcgtcat cagaaacttc aacaaatcgc
     2161 ggttcttcat atttaaagtt cgttaccaag c
//
LOCUS       DAWACS010000231         2186 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5100_length_2186_cov_2.405913, whole genome shotgun sequence.
ACCESSION   DAWACS010000231 DAWACS010000000
VERSION     DAWACS010000231.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5100_length_2186_cov_2.405913"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          626 a          355 c          460 g          745 t
ORIGIN      
        1 gttctggatg attgtagcgg catttttcgg aatggctaca aagtataccg agggtttctt
       61 ggctattaag tatcgtacta tagatgaaga aggtcacgtt cttggcggac ctttctatta
      121 catagaaaac ggtatgggta aaaagtggaa gtggcttgca aagatttttg cgtttttcgg
      181 tgtttgtgta ggtcttatgg gtataggtac ttttacacag gtaaacggta tcgcaagtgc
      241 ggttactaat ttctttgacc cgaatacaag ctgggcaatt catttgttcg gcagagatat
      301 ttcttgggtg gttgtaattg ccggtcttat tgtaacggta tgtacggcac ttgttattat
      361 tggcggtatc aagagaattg ctaatgtatc acaagttgtt gtaccgttta tggcagtgct
      421 ttatgttgta tttgctgtgt tgttgttact ttgcaatgtt acaaaaattc ctgatgcgat
      481 tgtgcaaatt gttcaaggtg cgtttggata cggtgacggc atacagggaa tcagagcggt
      541 tgccggaggt gtacttggtg caatgatggc tgcaatgcaa aacggtgttg caagaggtat
      601 attctcgaac gaggcaggtc ttggttctgc tccgattgcg gcagctgcgg caaaaactaa
      661 ggatacagtt cgtcaaggtc ttgtttcaat gacaggtaca tttattgata ctatcgttat
      721 ttgtacaatg acaggtcttg caatcgtaat agcaggttca tgggcaaatc ctgacttaaa
      781 gggtgttgcg ataacgacag aagcattcca aagcggtctt ccgttcccat cggttgtgtc
      841 atcatttgtt cttatgattt gtcttgcatt ctttgcattt acaacaattc ttggttggga
      901 ctattacagc gaacgttgtc ttgaatacct atcaaacaga aataaaactg ttgttaaggt
      961 ttatagatgg atatatgtac ttgcagtgtt catcggtccg taccttactg ttgcggctgt
     1021 ttggactatt gcagatattt tcaacggact tatggcgata ccaaacgtaa tcgcacttat
     1081 cgcacttagc ggtgttgtcg cgaaagaaac gagagattat tttaaagaat ttgatagatt
     1141 gtcaaagtga tttaagcggt cggaaacgac cgcttttttg tatgttagaa aagaatgtat
     1201 aaaaataacg gtttattctg taactaagaa taaaccgcta tcggattgat tatataatta
     1261 tttgtttttt atagcctcta ataacttgat aatctcggca aagcttaagc aagtaattcc
     1321 acttgttaat gtaattcccc aataaattag agcagtaaaa aatgaaaaat cgctatatct
     1381 atcagcattt tcaactccca tataaatgcc aagaataaaa ccgagaataa aaataataca
     1441 agctataacg gttaatatat tggctataat attgttgtta gatattactt taaattcgtt
     1501 ggtgtttatt gcttctttta ttgattcaag ttcttcatcg gttaaagaaa ttggttcctt
     1561 tttatatttt cgcaaacatt ttaaggtgga atcatattca gtaaatggat attcagaata
     1621 ttcatctttt tgtattatat ctaatttgtc aatagtaacc ttaacttttt cattgttatc
     1681 ggtaatccaa atttcatcac caacttttac attggtagaa gacttaactg tgaaatttaa
     1741 agtcttattg ccggtatgcc catgacaaac tatttcaaat ggagcatata tggtgttata
     1801 tataccattt cttgttaaat aatcagatat ttttttcttt tcaattaatt ttttggattg
     1861 taaaaattgt tctacctttt cgttcatatg tattcctccc attaaaaact tttcataatt
     1921 atatcattat tagatatagt tgtcaatatt gatggagtag ggtcggcgcc cacgacgtct
     1981 cgattaggag atttgcaggg gcgaccattg gtcgcccgtt aagagagata accgaaagaa
     2041 cgtcgagcca cacctcagtc aactgtgttg acagctcccc tcaatgggag ccttttgaaa
     2101 gcctctcctt gaggagaggt gtcacgaagt gacggagtgg tggcaatata aaaaatccgc
     2161 aaaaaaacag gtatgcgttg cacacc
//
LOCUS       DAWACS010000232         2180 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5125_length_2180_cov_1.536000, whole genome shotgun sequence.
ACCESSION   DAWACS010000232 DAWACS010000000
VERSION     DAWACS010000232.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5125_length_2180_cov_1.536000"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1865..1874
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          653 a          485 c          338 g          694 t
ORIGIN      
        1 gccatattta tcggagtgtc tgttataaca agacgaattg gaagtttctg ttctatccaa
       61 gtatcaatct tatacacata ctcccagcct ttcatagaag tatcacgcaa ataaggataa
      121 tcacgaaccg gaaagaaact tgaaattgta atgcctttta gttttggaac acctataagc
      181 tgaagttcac cttgtgatac tgtttcaaaa atctctgttg attgcggttt gcttatcgta
      241 aatgacgatg gcagcacagg cagacacatt acatcggctc ggttatttac acttaaatat
      301 atatccataa attcctccat aacaaaaaga gccactcaca taaaatgagt aactcttatg
      361 taactgtatc atattattct cacacaaaag aaacgtcttg cacttgcgtt tcccaaaatg
      421 caagtccggg tttgcaatat cttgcctcat aacatttacc cggtatagca tgaaaccttg
      481 ctggcgaacc accgcttata attccgagaa ctatagtaac atctacggtt gtaggttgat
      541 taattgtaat agttgcaact gaacccactg gaacttctgc tacaactcta ccacttatag
      601 caaatttcat tcttttaacg tgcctatcat ccgataagca tcttattttt acagttgttg
      661 gcatatttgc aatcggacat ccgcagttcg gacaagaacc tgctttatct gatacttgtt
      721 taccacaatc aggacatttg ataagtgcca taatatatat atcccctttg tatattttat
      781 tttattatac catatatccc tttaaatttc aacatatatt tgcatttctt tacatattct
      841 caatactctc aacaatcttc tttgcaacag tatctgcaat ttcatcagca tttgcaccat
      901 cagtatttac cgttacactc acatagttat ttacattagg tgttttatcc ttattcaagc
      961 tattcatcac ctgtgcaagc ttactccata gcatatcaag tggaagtata gcctccgcac
     1021 ctgcctcgcc accgaccatt gcgtttgagc cgtttattcc aaacattgtg gggcgtgtca
     1081 taataccgcc cttcctatac cacgcaacac caaagctcgg cactgatggc ggattcaaac
     1141 taaacgaacc gcttatcttg aagtgcggta gtttaatctt tggtaaagac caactgaaat
     1201 taaagtaccc ttttatagta tttatagcat ttgaaatggt agatttcacg ttattgaaaa
     1261 tagaaactac tttgtttttc acattttcaa aggtgtttga tatagtaaac catacagacg
     1321 aaaatgtact tgtcacatta ccaacaatag agctgatatg tgtttttata cttgcaatct
     1381 tactgctgac cgcactgacc acctctgaaa atttactctt tataccctcg accatctgac
     1441 cgagcttacc gcctgtaagt gcgtttatag catcgtaacc tatggtgtaa tattccttta
     1501 atcctgtcat atatccggca acaacacctt taataccacc gccatgctgt gtgtacgcat
     1561 ctttaatgtt attaagtttt tgtgatgcga tattttttgc accttccata acatttccga
     1621 taacattttt tacggcagta attttttcag taacagcatt tttaattccc tcaaacacat
     1681 tctttattgt gttccaaagg ttcatagcaa agactttgac agtatcccaa tgcttataca
     1741 aaagtacacc tattgcaata accgcaccaa tcgcaatcac caccaagccg atgggtgaag
     1801 ttacaaatgt cataatagca ccgaaagctc ctgttacaac attaagtgcc tgagtagcgg
     1861 ctatnnnnnn nnnntgccgt aaccgcacct gccacgcctg caatcacagg cgatagtgtg
     1921 cttgcagcag aaataactcc cgatgctata tttaaaacca acgataatga attacaaagg
     1981 ttcggtacaa tcacatttgc gagagtttgg ataagtgccg tcccattccc accgaacgca
     2041 ttaacaatag aattcttaac attatcaaat gcagaccgca aattctctat tgcgggagag
     2101 ttgttttcga tagccctttt tactgcatta aatacagtac ttgcaatata gtagattgta
     2161 tttattgcac tgaatagtcc
//
LOCUS       DAWACS010000233         2172 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5140_length_2172_cov_2.083609, whole genome shotgun sequence.
ACCESSION   DAWACS010000233 DAWACS010000000
VERSION     DAWACS010000233.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5140_length_2172_cov_2.083609"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1695..1704
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          699 a          328 c          553 g          582 t
ORIGIN      
        1 ggtaatttga atgtaaatga aattatcgag gacgagggta tttataaatt ttatgccgac
       61 tatcagccga cttgctggat ttggggcgaa tataaattga ctcacggtaa gcaaaacgtc
      121 agtgcggcac ttgaaaattc gtgtaactat ttcttctatg aagtcggtag acgtatggga
      181 ataaataaac ttgatgagta tgcaaagaag ttcggtcttg gtgaaaagac gggtatcgag
      241 cttgacgaag aagtggcagg tcatatggca agccccgaat ataaaaaaga ggtagttgca
      301 agtgctaccg atcgggaatg gtttggcggt gacactcttc aagcggcaat cggtcagtca
      361 tacagtttgt ttacacctat tcagcttgca aactatgctg caacaatcgc aaacggcggt
      421 acaagatata aagtaaatct tataaaaagc gttcgttcat cggttgacgg cggtgttgta
      481 aaagaattta atccggaagt tgtcgaaaaa gtcgatatgg acgatgaagt tatcaatgcc
      541 gtaaaacaag gtatgaaacg agttgttgac gaaggtagtg cgagtgaaat tttcgcaaat
      601 tacggtatag cggtcggcgg taaaacaggt acggcacagg tcggaaacgg ttcaaataac
      661 gctgtgttta ttgcttttgc accgtttgac gatccgcaaa tcgcggtatc ggttgtgctt
      721 gaacacggtg ttcgtggtac aaatgccgcg caggtagcaa aagatatttt tgataaatat
      781 ttcaatcttg acagtgcaga cacaaccgca gagcctacaa ccgcaacaga tgtgcaaggc
      841 ggattattga gatagtttat aatatgggga ggtttgatta aattggaaaa agagcttata
      901 aagctgaaag gtacggctga cggggtgaag atatatcttg acagtgaatg tgaattttct
      961 aagttgatga actccctgta tgaaaaaata agacagttca gaaagttttt cggcggcgga
     1021 cgttgtaata tatatgttgt cggcagggaa ctttcaacaa gtgataaaat gcgtcttgac
     1081 gcggttatta cggctatgtt gccggaatgt gagataaatt acggtgaaag aaaagtgata
     1141 aaagccgaag attcgtttga agaaactctt gaattgccta aggaacttct tcaggaaaat
     1201 actgaagaaa tacataatga gagtgaagaa gtccgcgaaa ttgctgagga agaaacggag
     1261 cttgaggaga taaaggaagt cgtaacgact aattttaaaa gcagtcgtgc gaggttttac
     1321 gaaggcgttg ttaaagcagg caaaacagtt tttgcggacg gacaccttac ccttttcggc
     1381 gacgttgagg agggcggaat gataacggcg gtcggtaatg ttatcgttat cggaagtata
     1441 aaaggcagcg ttgaggcagg gtgtatgggt aatgacaatg cctatatatt tgcgcttgac
     1501 tttaagccga cagacgtaag aatttcaaaa actcactgca attttgatga atttgatacg
     1561 ccgaataccg aaaatcctaa aaaggcatat ttaataaata atcaaatttg tgtagatgat
     1621 tttttggtaa aaatatagac aaagagaaaa aaatgtgata taattacgca gaaaagagtt
     1681 ttgggtaatt ttacnnnnnn nnnncataaa ttcatattga aaggataagg aaaatgggaa
     1741 aaatcatcat agttgcatcc ggaaaaggcg gtacgggaaa gacaactgta actgcaaata
     1801 taggtgcggc attggctatg aggggtaatc ttgttgcact tgtcgatatg gatatgggtt
     1861 gtcgaaatct ggatattaca ctcgggcttg aaagcagtat cgtttacgat atatttgacg
     1921 taatcgaaga aaattgtgac ttggacgagg ctttgattaa ggatacaaga tatgaaaact
     1981 tgtattttat acccgctccg caaacccgtg acacttcatc tgttgaagat gaggcggtaa
     2041 aggggatata ggaaaaactt tcttcaaggt ttgattattg ccttgtagac gctccggcag
     2101 gaattgacgg cggtttttta tatgcggcta tgggtgcaga cagtgccata tttgtcacaa
     2161 tgcccgaagt ta
//
LOCUS       DAWACS010000234         2166 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5155_length_2166_cov_1.581241, whole genome shotgun sequence.
ACCESSION   DAWACS010000234 DAWACS010000000
VERSION     DAWACS010000234.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5155_length_2166_cov_1.581241"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    499..576
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1097..1106
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          581 a          471 c          326 g          700 t
ORIGIN      
        1 tttcaattcg aaatttcata tacctttttt acatctctta acataaatct taaaacacgt
       61 ctttttatcg caatatccgt attattcaaa tcttcaatat taacagcaca atttctgatg
      121 tgatgtttat aaaaatcttc cgcaataccg tcaagataat cactgtcatc ttttatcaag
      181 caagcatttt ccgtaacggt tttaataaaa ctcgtgttaa attccttttg aatatacgga
      241 agtaatgtgt gacgtatttt attgcgtgtg taatcttctg tcaaatttgt actgtctgtc
      301 atatattcaa gtccgttatc actgcaatat ttttcgattt catctcgtga aacattcaat
      361 atcggacgaa ttatatttcc gcggacatac ggtattccgc aaagtccgtt tgtcgtactg
      421 ccacgcataa aattcataag caaagtttcg gcattgtcat ttctgttatg cgccgttgca
      481 actttgttta tatcgtatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncatt taacacaaaa cattcaatgc
      601 tcaatttttt tgaaaagttt tcggcaaaaa gttcatctct ctttgcctcc tcgcctctta
      661 tgccgtgatt aatatgagcc gtatataatt ttatgtcaaa ttcatttttc agcgaccaaa
      721 gtgcgtgagt aagacacacc gaatccgcac cgcccgaaag accgaccagc acgctgtcgc
      781 cgttattcaa taaattatat tcacgcaagg tctttttaat aatatctaaa accattattc
      841 ttccttcaat gcaaattcaa cattagaaaa cttagtatat tcacctttcc aaccaagttt
      901 aaacataccg gtttcaccgc ttctgttttt tgcgacaata cattcagcca aatttttatc
      961 ctcggtatcg ggattataat attcgtcacg atacaaaaac ataaccatat cggcgtcctg
     1021 ctcgatagca cccgattcac gcaagtccga aagcatggga cgcttatcgc ttctcgattc
     1081 agccgcacgc gaaagcnnnn nnnnnnatta actgcaaata gtcgattaca ataagttcaa
     1141 gattttttgt ctgctttaat cgtctgcatt ttgcacggat ttccgatacc gttattgacg
     1201 cagtatcgtc tatatacatc ggtgcagttg caactctgtt tactattgca ccgatttttt
     1261 cccaatcttc gcctgttata tcacctgttc tgattttatt tgaattaaca agtgcctggc
     1321 tgcatataat acggtttacc atctgttctt tcggcatttc gaggttgaat atcgcaacgg
     1381 tttttttatc ggtaatactg acgtgttccg ctatatttac cgcaaaactt gacttaccca
     1441 tacccggacg tccggcgata agaataagtt cgccgccatg aaaaccgccc gtacgtctgt
     1501 taagttcatc aaagcctgtc ggcaaacccg ttataccgct ttgatttaaa gagttttcaa
     1561 caagttcctg ataactgccc atgaatatct cacttatagg aagtatatcg ctcttgttgt
     1621 ttgatgatgt aacgtcaaaa ataagctgtt cggcttggtc gactattctt tcgactttat
     1681 cgttctgacc gtaagccata tcggaaatat cgttggcact tttaataagt gttctcaaaa
     1741 ctgccttttc cttaattatt tgcgaataat acgtcacgtg ccttgttgtc ggcacatttg
     1801 ttgccgcatt tctcaaataa gcgatacctc cgacagcctc aagtttgtcg ctttggctca
     1861 acttatttga aacggtaata aaatctatag ccgcattttc gttaaataaa tcaagaatta
     1921 cactgaatat ttccttattc tgtgggaagt aaaaatcaga cggtctaagt atttcaaccg
     1981 actccgcaac actgtgcgaa ttagtaagcg cactgcctat tacagccaat tccgcctctt
     2041 gactatatgg cagttctctg cccattacgt caacaggatt cgacatttct gccataaatc
     2101 cattacctcc ccaaagttat atctgttcta caactacgtt taacttaccg acaacgcctg
     2161 tataaa
//
LOCUS       DAWACS010000235         2161 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5170_length_2161_cov_1.797721, whole genome shotgun sequence.
ACCESSION   DAWACS010000235 DAWACS010000000
VERSION     DAWACS010000235.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5170_length_2161_cov_1.797721"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1715..1724
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          547 a          512 c          373 g          719 t
ORIGIN      
        1 ggatgtaacg tcccctctga catatgtgcc ggaaatgccc aagtcttttc agcgcatata
       61 tcatttataa ttcgtaaaag cgtctcaaaa tgttcatctt caccgtaaat aaagcacatc
      121 atagccgaca ttagcagtgc gccccgacgc acaaaatact tttgttcata ttcctgtcgc
      181 tgacctgttg cataaaaata gttaaataat tcctcagtca gttcaggcat ttccatatct
      241 ctgaattttt tgtactctga ccttatataa gctaaatttt tctctaaaca agacatattt
      301 tttatagcct taaaatcatt tacctgcata cttttacccc tttatagaaa acaccacaga
      361 acagatagct ctgtggtgtt ttattctgca ttattctact attttattta taatttcaat
      421 tgatttgata agagttcctc ctgttgcagc gtttgttaca aactgtatat acgaatcgcg
      481 cttcgttgag cctccgacca tatagaaatt atccaaatcc acttcagcag ttatccattc
      541 gccatctccg cttccaagca ctattctctg ctgattgtct tccgatgtca tcaccaagcc
      601 gttttcttcc tcgttaagct cggtacattt tataaacatc gtacccgcgt tctttttata
      661 tgttactcgt acctttatag gagtttttac accatcagat acagactcac tcttttgcgt
      721 aagtccgaga taacggtaat ccggtactat agaaaattcc gcgcgctcat tttcctgtga
      781 tgattttatt ctgataccat atgtgccgtc atcaagtttt tcaggtacaa aggaggttac
      841 atcgtttgtt ttccacgttg caagcgagct gttgaacata ccgttatacg ctgtcaagcc
      901 cgaacgaatg cctgttgctc ctgtcgcgcc catatcctcg gttctgtcct catgttcgct
      961 gccgtcaaac aactcaacct ttgaaatttt gatagtaccg tcacttctgg tcaatctgat
     1021 agttatatca ctgtccattc caccaaattg agcatccgga ataagaacag tagttgtttc
     1081 ccagcggttt gttccggctg tatagaaata gtgcgcgccg tctatagcgg tatttatcat
     1141 tgaaactctt acaggtatat cagccatgcc gccatcatga tatgttatgg taacaagtct
     1201 gtccttctga cctgatacaa catttttgtc aaccgcaaaa tgaagtgatt tatcggttcc
     1261 tgttatagta gccgccccgt cgtcaactgt tattccctcg tcagccgtca ttgtaagcgc
     1321 ttctgcatta tcggggtcag caaagctata ttctcccagc tttgtgctgt aatcacggta
     1381 agttacctca attttgccga taattatatc ctcattctca ggaacagccg aagcaataaa
     1441 ctgaataggg ctgaatgtgg attctgaagt tcccttaaca tagtaatcat caacattaat
     1501 ctttactgtt ttccattcgc cgctgcctat gagtgagagc gaagcgtcat tgtcaggcca
     1561 ataaatgctg ccgtcttctg cctttttctt tgcacccttt acataaagcg caccgtttgt
     1621 gttgttcgcg tagtatgtaa ccttaactgt tacaggtctt acctgcgctg cattttcctc
     1681 ggtataatcc ttttcacttg gaagtttcgc attgnnnnnn nnnnccatat gcctgaccca
     1741 aaaaacgata atccggtaca agcctgattt ctgcgcgttt attaccgtta ttaacatcag
     1801 ttgagtttag cttaattgct ttctcgccat caacctcggc aacctctacg ctcatttttt
     1861 catttgctgt gcttaacgaa gccattgtag tgttaagcat atcatctgca acagttacac
     1921 ctgaaacaat accgttcaaa tagtatccgc ctacttcata tgacggacag tctgaaagct
     1981 ttacatctgc tattgcatat ttcaggttat ctgctgattt aagctgaata tcttcagcat
     2041 tgttattgaa tgacgcgtca ggtattaata ccgatgctgt ttcccacttt cctgttccgg
     2101 ttgtgctgaa ataatgcgcg cggtcaatgg caggatttat cattgaaaca cgcattctca
     2161 g
//
LOCUS       DAWACS010000236         2158 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5180_length_2158_cov_2.212078, whole genome shotgun sequence.
ACCESSION   DAWACS010000236 DAWACS010000000
VERSION     DAWACS010000236.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5180_length_2158_cov_2.212078"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          508 a          540 c          431 g          679 t
ORIGIN      
        1 cttacaagct ctgacacaat acctggcgtt gttccgtagc cgttaagcat taaatcgccc
       61 gcgagctgag agcgtcccgc atttccgtaa tgattatggt cgccgaagca atcaataatt
      121 gcctgtgccg ctgcattttc gtcagtatag ccgttctttg catagtccgc gacatattca
      181 ttaaacgcat tatcggcgtt aagacctata tcaataaacc cgagaatatc gggattatct
      241 tttctttcct catagataac tcttataccc aaagagtcat acgcgtcctc acggcaaccg
      301 tgaaatgtgt gtgttgtctt atcatacaca tactcatatc cctcaacgca gccgtgcgga
      361 gtatagcttg taagtatcac ctttgcgccc attgcaaggc acgcgtcaac ataataatca
      421 agcggagctt taaactgtgt gcctgacata ccgccgggat ttgttcccat atttccgatg
      481 gtaacaatat ctcccggtcg tatgtttatc agaatattat cgagccagcc ctgcgtataa
      541 tatgttttca gatttcttga acctttaccg ttattatggt aatcggcaag cgcggtaagc
      601 tcgggatagt ttgcttggtc gcgcgctaaa ttataagcgt acgaaccgtt gtttccgata
      661 gttgagtcgc cgacagacca gatattaggt ttttcggcgg cggttttttc aaccttttct
      721 attgtgatat aagcaacctt tgcgcccgtg aatttcaagt caagcacatc gtccacaaca
      781 ggaatatcat atatctgttc cgttatttcc gctgttttaa cggtatagcc cgtatgcgtt
      841 gtgccctctg cctcaagtgt tctttcatga cctgataatg cttcgcttac atattcagat
      901 accaaatcac cgctaaacgc aacctttact ttatatagct ttccttttgt tacctttgct
      961 ttaaatgtaa aatcgcctga caaataatct gatgttgcat tatcaatgct cggcgcgccc
     1021 tccgacttca cactgccctc aattccgtag cctctctgct cggtataagt ggttgacgga
     1081 ttaacgtctg tataaccctc tgtaacactt tcgtcctcac cgcttccaaa gtcaaaggac
     1141 agaccgtgaa cagtaacctt aacgcttctt gacaatgttt ttccgtcact gctttcacct
     1201 gtagctctga cagtaaatac gcagggaacc gctgctgacg taacgcttac aatacccttt
     1261 gatgcgtcaa atgatatgcc ttcgggaagt gtgtacttat tcacattatt tctgtcatag
     1321 atttcgagcg tcacatttct gcctaaatct gcgccctcgc cgttaatgac agcggcgctg
     1381 tatttagctg ttgttaccga tttttcgtca agcggtatcg caacgcttcc ttgtgatgat
     1441 gtaaacttta tactttcagc cgagtcggta atattgagct tgacggtttt tgtatagccg
     1501 cctatactaa cctgaacagt cgcttcacct gccgaagcac cctcgccgag agttatagtc
     1561 gctttgtgag agtcaagctt atcaggcgtt attataacag actgcatatc ctcctccagc
     1621 actctccact ctgccgcgcc tgttatgtca tagccctccg cgcttttaac cgacgcggat
     1681 agtgtcgctg tgtccttatt ggggattgac agcgtttcct gcgatacgct gagcgtatag
     1741 gttgacgcgt ccgctgtggg gtagtatgcc ttatatgcgt caaaatcaat agttcccgtt
     1801 accttgtttg cacacccgat tgaatagtag gtcggctctg atgtttcagt ccacgccacg
     1861 cttccggttt cgccgacaag attattgtct aaatcataca cctttgcaaa acagctttcg
     1921 gtagttttat cagccgacaa tacaatttta taccacttgc ctttatcggc ggtaacatct
     1981 acatcattct tttttataga cgttccgttc agctttattg tcgtgccgtc aaattcaagc
     2041 gttacgggag tggtgtaacc gtccttatcc gtccaatacg gctgttttga tgtaagcgta
     2101 atacagccgt ttccgttaaa tcttatattt tgttcaaata taatctgctt ctgcggaa
//
LOCUS       DAWACS010000237         2152 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5193_length_2152_cov_3.001431, whole genome shotgun sequence.
ACCESSION   DAWACS010000237 DAWACS010000000
VERSION     DAWACS010000237.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5193_length_2152_cov_3.001431"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          735 a          329 c          498 g          590 t
ORIGIN      
        1 tcatcaataa gtattatttc aagattttta tatgtttggt taagtatgct cataacgcat
       61 ttttcgatat atttttcagt gttgtataca ggcactataa cgcttatcaa tttcatattt
      121 tccacctacc ttttataatt aagattataa ctgattataa taattaagtc aataaaatca
      181 taaatataac acacaatgaa atacagaaag gatatgaata tattatgttt cgagggtata
      241 atttaaaaca aaaagaagtt gtgaatataa agacggcaga gcggcttgga cgcatcagtg
      301 acgtggagat aagcgagagt acggggaaca tcgaagctat aatagttccg cgaaaagggt
      361 gctgtttggc aaggcttttc ggaggggggg aacttgtcat tccgtggagc gctattgagg
      421 tggtcggaga agatttggtt cttgtgcgat tttttgatat tgataatgaa aaattatctt
      481 gaaaaaaatt atcgaatagt atataataat accataaata ctatacataa aggagaaaca
      541 caaatgagat gtccatattg cggatttgcc gaaagtaagg tcattgattc aagacctacc
      601 gaagaaagca attccatcag aagaagacgt gagtgcttaa aatgtcaaaa gcgtttcact
      661 acatacgaaa agcttgaggc gatttcgctt gcggttataa agaaagatca gtcacgtcag
      721 cagtatgaca gaggaaaagt gttaaaagga ataattacgg cttgtgaaaa acgtcctata
      781 tctctatcgc aaatggaaca gattgcggac gatattgaaa gcgaattgta tcagtcaatg
      841 acgcgtgaaa tcgaaagcac cgctatcggt gaaaaggtta tggaaaagct taaaaaactt
      901 gatgaagttg cttatgtacg ttttgcgtct gtatacaaaa gttttgatga catagatacg
      961 tttatggcag aattgcaggg acttattgat gataaaaaat attgatttgc atacgcattc
     1021 aactgcgtcc gacggtacgt ttactccgtc ggaggttgcg cataaagcac ataaaacagg
     1081 actcagtgcc gtcgcactta cagaccacga tacgataaac ggacttgccg agttcaaacg
     1141 tgcttgcggt gaatacggaa ttgagggtat atccggcgtt gaaataagtg caaagtacga
     1201 aaaagaaatg cacatagtcg gcttgtttat tgatgaaaac gatacggaac ttgccgaaaa
     1261 acttgacgaa ctaaaaaatg caagagaagt gcgtaacaaa aaagtacttg aacttgtgaa
     1321 caggcaaggt atggaaatat cggttgacga tatactgtcg caaaaagacg gtgcaacact
     1381 tttgaataca ggcagagcac atattgccca tgctatggtg aagaagggat atgttgcgtc
     1441 agtagacgag gcattcaaaa agtatctcgg caagggtatg agctgttatg ttccgcgaaa
     1501 aacgtattcg cctaaagaaa gtattgaaat gattaaaaat gcaggcggtt tggcgatttt
     1561 ggcacaccct gtttatatta cggaagatta cgacaaattg tattcgcttt tgaaacaatt
     1621 aaaggaatac ggacttgacg gtacggaatg tttatataac tgctattccg aagaattttc
     1681 aaaaatgtgt tttgatatat gcgataaatt aggacttgta aagtccggcg gaagtgattt
     1741 tcacggcggt aataagcctg atgtggagct tggaaaagtt tcgggcgatt atgtgcctta
     1801 tgaatttttg ctgaatatga aagaacaaag gggtctaatg taatagacct ctttatttgt
     1861 aagaaaggac ttgataaaat ggaacgaact attgcggcaa tttccactcc gcagggtaac
     1921 ggcggtatag ccgttataag aataagcggt aatgacgctg ttaaagttgc ggacaaggtt
     1981 ttcttcggaa gtaaaaaact tgccgattgc gatacacata ccgttcatta cggttttata
     2041 aaaaacgaaa agggcgaaaa ggttgacgaa gtgcttgcaa cggttatgct tgcaccgaaa
     2101 acttttacgc gtgaagatgt tgtggaaatc agcactcacg gaggaagtac ag
//
LOCUS       DAWACS010000238         2146 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5211_length_2146_cov_2.077953, whole genome shotgun sequence.
ACCESSION   DAWACS010000238 DAWACS010000000
VERSION     DAWACS010000238.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5211_length_2146_cov_2.077953"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1550..1559
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          593 a          466 c          379 g          698 t
ORIGIN      
        1 aacaactaag ctcaatattg taattgtcac gcccattgaa agtccgaaac ccggtattac
       61 tcttttcttc tttgctttca tttacattcc ttctttactt atctgttttt tcataaatgc
      121 ggtcaaatac tccgccgtca tcaaaatgct ttttctgtac ttcgtcccaa ccgccgaagt
      181 cttgtattgt tacaaggttt atgttaaggt caaatgtatc tgcatattcg tttaatatat
      241 cagggtttga cggacggtag aaattgtcgc ctgcaattct ctgtgcctca tcggaatata
      301 aataacttaa atattccgtt gcaacatctc ttgtacctct ttggtcaacc accgcatcaa
      361 ctaccgcaac agacggctga gccaaaatgc ttatgctcgg cgttacgatt tcatattcat
      421 cgggattttc ctgaaccgat aggaacgcct cattttccca agcgataagt acatcgcctt
      481 gattgttttc aacaaatgat gttgaagcac ctcttgctcc cgaatcaagt acaagcacgt
      541 tttcgtataa cttctttata aatgcctcaa tctgtgtttc gtcaccgttg taaagtttat
      601 cggcatatgc ccaagccgca aggtaattcc atcttgcacc gcctgaagtt ttcggattag
      661 gtgttataac tccgacgccg tctttaacaa ggtcgcccca atccttgata tttttcggat
      721 tgccttttct tacaaggaat acgattgttg aagtgtaagg cgaactgtct ttgtcaaatt
      781 catttatcca gccgtcctct ataagtcctg cgtcttgtat tgcctttacg tcatattcaa
      841 gtgcaagcgt tacaacgtct gcctcgtttc cgtttgcaac ttcaagtgct tgcttaccgc
      901 tgccgccgtg tgactgtata acttcaacgt catgtcctgt ttgccctttc caatgctctg
      961 cgaaaagttc gttgtacgca ctgtataatt ctcttgtagg gtcgtatgat acgttcgtta
     1021 tcgtaaccga cttttctgta cttgtgtctg cactgtttcc gcagcctgca agtaccgtta
     1081 ccgccaaaat tgatgttaat aatatactaa aaattttctt tttcataaat tcattacctc
     1141 gctataatat aaaatttatt tttttatcac ctctgagcca tacacattcg gcaacacatc
     1201 tttctatata ccatgctttt tctgttcaaa ttttctctca taatcgttgc ctcctttgtt
     1261 attcatactt ttcctatggg atataggatt tttaatgtcc ttatcttact cctatattgt
     1321 cgttttgtca acattttcat atagtaaaaa aattaaattt ttttcctata tgttttattt
     1381 cggacgaccg atgatcgtcc gctacgaaat acttataaat taagaaaagc aaaattatcc
     1441 gaatataatt aatcatactt gcaaataata acacaaaacg aaaggcggaa atttttatgt
     1501 taagaggaaa aatagtagta gtcggtgccg gttttgtggg ttctacgacn nnnnnnnnnt
     1561 tctacaaccg catacacaat aatgcttgac ggttttttca gagaaattgt attgattgac
     1621 gtgaacaaag acaaagccga cggtgacgct cttgatatgg ctcacggcac atcttttcta
     1681 aagcctgtaa gcgtttacag cggagattgg aaagactgtg ccggcgccga tatagtcgtt
     1741 ataacagccg gggtaaacca aaaagtcggc gaaacacgaa ttgacctgtt aaaacgaaat
     1801 accgaaattt ttaaaagtat agttgaaaat gttatgaagc acgcgccgac agacgtaata
     1861 cttcttaccg tcacaaaccc tgtcgatatt ctgacatacg tcacctacaa actttcggga
     1921 cttcctaaac agcaaattat cggctcgggt acggttcttg acacttcaag actgaaatat
     1981 cttatcagca aacatacggg aattgatgca cgttactgcc acacatacat tatcggtgaa
     2041 cacggtgaca gtgaagttgc ggcatggagc atcacaaaca ttgccggtat gagtatgaaa
     2101 agtttttttg agcattacgg caagtgtaca gatgaagatt tacaag
//
LOCUS       DAWACS010000239         2117 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5280_length_2117_cov_2.350630, whole genome shotgun sequence.
ACCESSION   DAWACS010000239 DAWACS010000000
VERSION     DAWACS010000239.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5280_length_2117_cov_2.350630"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1228..1237
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          460 a          554 c          368 g          725 t
ORIGIN      
        1 actgacaact gtgacgcagt tgtttcggct tttctgtcaa attcttcttg tagtgccgta
       61 ttttcttggt atgcggtgtt tgaacgattg acactctcgg ttactaaatc ataaccgttg
      121 actaatgcca tcatagcctg tatatcctgt gtattgttta tgcctaaatc atctaacgca
      181 acagttaggt tctcggcaga ctgcaagcct tttaatagtc cgttaaatgc accggagctg
      241 tcagtattcc actgctcttt aaattcttcc gcacttttac cgctatactt tgcgaatttc
      301 gtcaagccct ctccgccgct tgcaacggct gtttctatgg atagccacgt acgacctatc
      361 gcactaccgc ccatttgtgc ctcaatgcct aatgatgata gtgcggcaga ataacccaac
      421 acgtccgccg ccgacattcg tacagatgaa ccgtatttac ccatacgcaa tgccattgcc
      481 gcaatctccg attctgttgt cgcactgtgg ttacccaaat caacgattgc actgccgata
      541 ttacggattt cgccttgact tgtacccatt acattcataa atcgggcaag tgtagccgcg
      601 ccttcttcgc cgacaaggtt tgttgctgaa cccatttgtg ccattacttc cgtaaagtcg
      661 ataatgtttt cttgggatat gcctaactga cctccagccg ccgcaagctc atttagttca
      721 gttgccgtct gtggtatcgc gcctctgccg tcaatacctg ttgttgacaa atcgataatg
      781 ccttgcttta ttttggctaa ctgttccggt gtagcgtcaa ccgtcttttt aactccggca
      841 aaactatcct caaaatctat cgcaaacttg gcactcgcaa caccgcccgc ggcaagtgca
      901 gtcgccgcat actgtaacgg tttagttaca gtatctatac cctcaccgac ttctttccac
      961 cgcttacctg tattctgtag gttctgcgcc tcatctgcac gttcagcggc ttttaagcct
     1021 ttctcgtatt cctcgtattg ctccgttgct tttttgacgg ttgcttgtgc gtcggtatat
     1081 gcctttttac ttcccgacaa tgccgcctct tgcgtacgaa tagaatcgga tatactttga
     1141 ctttgctttg tgtatgcctc aatctcggta tttacccaat tcaatgcctt ttggttgtct
     1201 ttatacgaaa gactgttttt gtcaagannn nnnnnnnatc taattccgtt ttctgtgctt
     1261 tcagtgccgt aacattttta tttacagact taacgttatc ctcatacgct ttttttgtat
     1321 tcgtcaatgc cgtacggctt gtttgcaagg tggtttgcgc gtcttgcatt tgctttttat
     1381 atgccgtaag accttttgta cttgtattat tatttttgct ttgcgtctgc tccaattttg
     1441 acaattcact ttcaacactg cttattgtcg cctctaagtc ggacgcatca cctcttattc
     1501 ttactactaa ttccgccgcg tcagccatta caaatcacct cactacattc cataaaacat
     1561 ttttaaatac gggtcatttc ccgtatattc ttcttcctcg tcctcgatta taactgcaag
     1621 taataatcga gggtcttgtt ttgccaaatc attcggcaat ataccgtgat atttcagcat
     1681 tgtcccatat aaatcgctta atcttccttt tcggttgcct gctctggcag gctttcctcg
     1741 ttttttcccg taaaatcgtc cataaaccac ttcataactt cacgacacat tctcattttt
     1801 gctgaaacag ccgtgtccaa aatatcttgt gtcgcctctg taccctcaaa cagatagtca
     1861 acggcatctg cacataccga cgtagccgtt actttttcac cctctgcaac gtccatgtat
     1921 tctttttcaa ccaacgttgc cgcaccgaaa caccacggtt ttgatacata cttctttttg
     1981 ttgtgtacaa atgttaatac tctttgcatt gttacttgct cctctctata cgaaaaaagc
     2041 acgcctttcg gcgtgccttg tcttaaagtg ctttcttcac tggatagtag ttcatatcct
     2101 taaaccagtt ttcttca
//
LOCUS       DAWACS010000240         2116 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5287_length_2116_cov_1.882096, whole genome shotgun sequence.
ACCESSION   DAWACS010000240 DAWACS010000000
VERSION     DAWACS010000240.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5287_length_2116_cov_1.882096"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          605 a          484 c          338 g          689 t
ORIGIN      
        1 gtcttatcca tgcagtttgc agccgccatt acacccatac cgacataagg tattcccgaa
       61 agttcaaaaa gaccttgaat tgttccgtct tctccgaact caccgtgaag tacaggaaaa
      121 ataacgtcag gatgaatttt cgtaacctta tcaccttcaa aaacaagtat agccttatcc
      181 tctgcatcag gagaaagaat tgccttcttc ttgttttccg tatcctgttc ccattctccg
      241 ctttcaatct ttgtatagtc gcctgtgtac agataccatg caccgttttt tgtaattcct
      301 attgttgaaa tattgtattt atctttatta aggttattta aaaccgatgt aaccgatttt
      361 cttgaaattt cgtgttccgg tgactgtccg ccgaaaataa cacacaattc taacattatg
      421 taatcactcc taaaacttta tataatatta taataccttt ttcgtcacta aattgcaagt
      481 gtatttatta aaagtttatc tttttctcaa cttttcgagt acattttcaa aatattccgg
      541 tatatcactt gtaaattcca tcattttgcc tgtccgcggg tgtacaaaac caatcgtttt
      601 tgcatgaagc aactgaccgt ttaaattgaa cttttctttt tttattccgt aaagcgggtc
      661 gcctactatg ctgtgaccga ttgacgccat atgcactctt atctgatgtg ttctgcctgt
      721 ttcaaggata cattcgacaa gtgtatattg tccgaaccgt tcaagtacgt taaaatgcgt
      781 aaccgcctca cgtccgccga atacaacagc cattttcttt ctgtcagacg gatttctgcc
      841 gataggctta tttattgtgc cgctgtcctc ttttatattg ccgtttacaa gtgcaacata
      901 tttacgcatt gctttgcgtt ctttaagctg ttccgacaac ttcaaatgtg cctcgttgct
      961 ttttgcgact atcaaaagtc ccgacgtttc cttatcaatt ctatgcacaa tcccgggacg
     1021 catcacaccg ttaattgccg aaagttcgtc accgcaatgg tagacaagac cgtttacaag
     1081 agtaccgctc tcgttacccg gtgcagggtg aacgcaaagt ccctgcggtt tgttcacaac
     1141 gataacatca ctgtcctcat atacgatatt aagtggtata ttttcaggct caatagaaag
     1201 ttcttcgggt tcgggtacat ttattgttat ttcctccgta cccaaaatct tatatttctt
     1261 taaaagctgt ttttctccgc aaagcacaag accatcctcg cataattttg ccgcataact
     1321 tctcgaaata tccgaattgt ccgcaatgaa tttatcaagt ctttcgcctt tgttttcctc
     1381 tgccgcatta atttttaacg tctgcatttt tatcctcttt atcaaaccaa ataaaataaa
     1441 ccacaagcaa tatcgcaccg acagttatac ctatatcggc aatattgaat accggaaagt
     1501 ttataaatga cgtctgtata tagtcaataa caaaacctct gaaaattctg tctattgcat
     1561 tgccgaatgc gcccgcaaac atcattgtaa tcgatgtaca caaaagcgga tgtgtcggct
     1621 ttttctttat ccagtatatt accgcacaaa cgcagaatac aaccgaaata atgctcaaaa
     1681 gcgagagttt tcccgaaagt aatgaaaatg ccgcgccctt attttgaata tatgttatat
     1741 caaataaatt cagtatcgaa aatgcggtat cgcctacatt catacccgaa acgaccaagt
     1801 attttaccgc ttgatcggct ataattataa gcatcgcaat aataagccaa agcatatata
     1861 atctccttat tttgtaatat aatcgaatgc agcaaatatt tcgtcctttg aaacatcagt
     1921 cgtctgcata acctcgccga cttttgtcgg caaaacaaat tttaatttac ctgcaatttt
     1981 ctttttgtcc ttctgcatat agccgtaaac aacctctttt tcaggcaaat caacacgaat
     2041 tttaaaatga aagccggagc gtggacgcac atcgtgctga cgcaaaaggg gaacacggga
     2101 atcctctatg ccaatg
//
LOCUS       DAWACS010000241         2104 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5327_length_2104_cov_2.535383, whole genome shotgun sequence.
ACCESSION   DAWACS010000241 DAWACS010000000
VERSION     DAWACS010000241.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5327_length_2104_cov_2.535383"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          554 a          504 c          336 g          710 t
ORIGIN      
        1 cttcctgtgc tctgtcctcg atgtcgataa tcttcataat tagatcttcc attttcccaa
       61 cctctctttc ctctgcagat taatctgcat tatttcagac tatatttaaa aatcattacg
      121 attttgtctg cattaatttt tcaaactttg aagcggtgca ggaattctgc cgcctctgtt
      181 tataaattcg gcacttgaat actttttaac cggcattacg ggacctgttc ccaaaagtcc
      241 gccgaattca accacgtcac cctcaacctt acccggtgca gggataactc ttactgctgt
      301 tgtctttgtg ttgaccattc cgattgccgc ttcgtcggct ataatcgccg aaatagtttc
      361 ggcagttgtg tcgcccggta taacaatcat atcaagtcct actgaacata cacaagtcat
      421 tgcctcaagt ttttcaagag taagcacgcc gtctgttgcc gccgcaatca tacctgcgtc
      481 ctcacttaca gggataaacg caccggaaag tccgccgaca taacctgacg ccataatacc
      541 gcctttttta actgcgtcgt taagcagtgc aagtgcggct gttgtaccgt gagtaccgca
      601 catttcaagt cccatttcct ccaaaatata agccacactg tcgccaacgg caggtgtcgg
      661 agcaagcgac aagtctacga taccgaaagg tacattaaga cgttttgacg cttcttgtgc
      721 aacaagctgt cccattcttg taatcttaaa tgcagtcttt ttaatagttt cggcaacaac
      781 accgaagtcc tcgcctttaa ccttttcaag tgcacattta acaacacccg gaccgcttac
      841 gccgacattt ataacacatt caacctcacc ttcgccaagg aatgcgcccg ccataaacgg
      901 attatcctca accgcgttgc agaatacaac aagctttgca cacgcaaacc cttgcgtatc
      961 agctgtaagt tcagccgctc tcttaacaat ctgacccatc tgtttaacag cgtccatatt
     1021 gataccggct cttgtcgaac ccacattaac gcttgaacat acaagatctg ttgcggcaag
     1081 cgcttcagga atcgactcaa taagtattct ctcaccgttc gtataatctt tatgcacaag
     1141 tgccgtataa ccgcctataa agtttacacc tgtcttttta gcggctttgt caagagtttt
     1201 tgcgatttta acacattttt caacagtcgg ttcttccaat gcgtccacca aagtagctat
     1261 cggagttacc gaaattctct tgttgataat cggaataccg aattgagcct caatgtcctc
     1321 accggttttt acaaggtctt ttgcgtaagt tgtgattttt tcatacacct tttcacaaac
     1381 tgtatcaacg tcaggaccgg cacagctttt tagagaaata cccattgtta tagttcttat
     1441 atcaaggttt tcctcgtcaa ccatttttat agtttctatt acttcaaagg gattaatcat
     1501 ttatttgtac ctcccttata ttctgtgcat tgatgtgaag atttcttctc tctgcacatg
     1561 gattgaaagc cccatatcct tttcggcctg ttcaaatttt tcactgattt gctttactgt
     1621 aacattatca ttttcaaaat tcacaagcat tatcattgtg aacatatcct gcattattgt
     1681 ttgagaaata tcaagaatat ttactccgtt ttcaaataat atattactga ttttagcaat
     1741 tatgcccttt tggtccttgc ctattacggt aactactgct ttcatttatt tatctccaat
     1801 ctttcatgtt ttcattgttt atctttaatt gaattatagc actttcgttt taaaaaagta
     1861 agtggttttt ttaaagtttg tccactgtaa cagtttaata acccgaattt tattgcaatt
     1921 tgtttatttt ttcacaagcc gttttttgtc ttgatatttg cgacaaataa gccgtttttc
     1981 aaggctgttt tttgttcaaa aatacaataa aacctacggt taataagtgc ttttactttc
     2041 ttctttgtct taatgcctca tacatcaaaa cacctgccgc aaccgacgca ttaagtgaat
     2101 taat
//
LOCUS       DAWACS010000242         2098 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5344_length_2098_cov_2.426334, whole genome shotgun sequence.
ACCESSION   DAWACS010000242 DAWACS010000000
VERSION     DAWACS010000242.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5344_length_2098_cov_2.426334"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          567 a          483 c          373 g          675 t
ORIGIN      
        1 gtaggatgtc atggtgtcgt ccactactct ccgttttggg cgaccaatgg tcgcccctac
       61 aaaatgtttc gtgacaccat tccaacaaaa tatatttcca cacaaaaaaa ctccgaccga
      121 agtcggagtt tttaatctaa ttaataccaa gtttgtgaat catacttatc aagtacgttt
      181 ctgatattag ctattcttgt ttctgtatca ataagaccgt tatcgtcata agcacttgtt
      241 gcctttagca tagcatcttc tgcccagtga ccgtcaatgt ctacgattga atacaattca
      301 ggagttactg ttgtaccgtc ttgagctaca agacccatat ctgtacgtcc gataacatta
      361 ttgaacattt ggcagaattc tgctcttgtc attactctgt caggttcaaa tgttgtatca
      421 cttgtaccat tcatataacc gtaagcaact actgtttcga tatatgactt atactgtgaa
      481 tcagcaatat cagtaaattc agtttcgcct gtctttgtca gtcccaaacc gtatgcaaga
      541 agttttgcaa cttcgcctct tgtaactgca ccgatttcaa ctgaatcaac accgtcaaat
      601 gcaccctttt gagcaagata tgcaagacct cttacatacc atgtaccctc atgctttgca
      661 atgttatctg ttacaggata aactgtatct gttgtaccgt acttttggtc aaccattctt
      721 gtaatcatag ctgcaacctg ttctcttgta acagcatcgt caggagccat cttaacttct
      781 gcaacccatt taccgccgac tacgttacct tcttcgtcag ttacgtctac tctttgaata
      841 gcaggttcat aaccgaatat atacgcatag ccaaggcctt gaagatccac ttcttcgccc
      901 ggtgtaacag tcggagcagg tgtaggctct tcagttggtt ccggtgttgg ttcttctgac
      961 ggtacaggtg ttacgtcagg agttgtatta cctgcatgac cccaaattac cgcaccttca
     1021 ccgtaaatat caagtgaatt acagattagt tgaccgcggt taataccgtt acctgatgtt
     1081 gtaccaatct ttacatcagc tttcggagca tatactgtac cttcaatatt agtaccgccg
     1141 ccgtaaatgt tgaatgtttc cgcacttgtt acagtgttac ctaccatatg gcaagatact
     1201 tcatatgaag ttgcattgat tgcgtttaca ttacctgtaa ctctttcgtc agcatactga
     1261 ccgctgtttg caaatcttac tgtttcagcc tttgttacaa tgtcacctgt aatcttaccg
     1321 tcaaagtcaa actttgatgt tccgtaagaa taaatgtcac cctcaagagt aaatgtatta
     1381 ccgaatgata caggtgcgct gccaagaact atatcaccct ttgtcggagc attaaatgat
     1441 aaattctgcg gttcaccgtc atagtaaata tccgcacaaa tcatagagtt tgcacttgta
     1501 ctgccgattg aaacccagct gtcgccccat tgactcggaa cgataatcca tttcaaatcg
     1561 cctgtaccga aatcaaatgt accgtcacct gtgtcaacta cgtttaccat agcctcaccc
     1621 ggagcttgtg aaataagata tgcaggattg tcgcctacta cttcaatata acctttctta
     1681 tcgttagcag tagagaatga taattgatta acttttacat ataccggacc gtttgttgtg
     1741 tcgataacaa ccttaccgcc ttgaacgctt agtccgtcaa atgatgtatt ttctgaaatt
     1801 gtgtaatatg gataaccgtt ttcgtcggta ggaagactcg gatacgacca ggcattaacg
     1861 cctacccaac cttgtgtaga tgtgtattct acatcttcat aagcaggtgt ttcgggtttt
     1921 acagaatcat ctgtaagata agtatcggga taatatgcat caagatactt tgacatattt
     1981 tcttcaacct tatccacacc ttttctgttt gtgtctttaa ggattgcaga atattcgtct
     2041 tgatagctta cttcctgtga tgaaattata tcaccgtcaa gatagtttgc accggcat
//
LOCUS       DAWACS010000243         2086 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5393_length_2086_cov_2.032004, whole genome shotgun sequence.
ACCESSION   DAWACS010000243 DAWACS010000000
VERSION     DAWACS010000243.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5393_length_2086_cov_2.032004"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          634 a          388 c          428 g          636 t
ORIGIN      
        1 cggatatatc ggcacactta tagatgacct tataacaaag ggtacaaacg agccgtaccg
       61 aatgatgaca tcacgttcgg aataccgttt gcttttaaga caggataacg cagatgaaag
      121 acttactccg ttcggatata aaatcggtct tatatcggaa gaaagatata aaaagttcct
      181 tgaaaaaatg gatatggttg aaaatgaaat taagcgtgtg acgtcggtta tagtaccgcc
      241 gaaagacgca aatccgcttt tggaacagta taattcgacc gccgttaaga cgggaatacg
      301 tcttagcgat atgataaaac gtccagaact tgattatgaa aaacttgcac ctgtcgacca
      361 taaccgtccg acacttccgg acgcagtttg tgaacaggtt gaaattaaac taaagtatga
      421 cggctatata aaaagacaga tagcacaggt tgagcagttt aagaaaatgg aggaaaaact
      481 tcttcccgaa aatcaagatt attctgatat acacggcttg cgtttagagg caagacaaaa
      541 attgaataag atacagccaa agtcacttgg acaagcgtca agaatttccg gtgtttcacc
      601 gtccgatatg tcggtattga ttgtatggtt ggaatcacaa aaaagataac aaataaaaaa
      661 tgtgtgtcat ttgatatgac acacattttt agatagtagg caaaacagtt atggttttgc
      721 ctactttatt tttgggagtt tgaggtgcaa cctcattaaa aatcatttct ggcgacctgt
      781 gcggcagaaa tgacttaaaa tctaaagttt cgactttgga ggaactttat gattttttct
      841 caaaaaaata ctcaaaaatt acatcgaaga tgtgattttt ggagctgtcg acattttgtc
      901 gacaggctca ctactcatta tcatttacca tatgattaat tcttgcgggg atttcgtttg
      961 ttactatgaa gtccacaccg taatcaatgt atttttgtgc tgtttccgtg tcgtcaacgg
     1021 accatacacc gcagaaaata ttttgttgat ggagtatatc tattgcggct tgatctactt
     1081 gcggatagtt atacaaaata cctctgtttt cggatatttc cgtaatacgt tcaagattat
     1141 ccgtatacgg tgcaggagtg taaccgattg tcataacagg gataatactg tttcttgcac
     1201 gaatttcttt tacatcatca atcgcacctg tgattataca atgcgtttca agtccgcttg
     1261 ctatgattgt gtttagaaat gcatcgtaat tggtgatatt tttgatttcg ataatcggta
     1321 catttccgta tgtactgcat atcgaaaggt attcttcaag agtaggtatt tttaagtttt
     1381 cgtttgtgtc gaaataattt ccgtagtcaa tttggaactg acttaaatcg tcaagcgtgt
     1441 agtcggaaat agctcccgaa ccgtttgttg ttctgtcaag agtgtcatcg tgcatacaga
     1501 cgaattgacc gtcacttgtt tcgcaaatat ccgtttcgat accccaaaaa ccaattttac
     1561 cggcaagctc aaacgccgga atactgtttt cgggcgcata gtttgaatat cctctgtgtg
     1621 cgacaaaacg ttccgtgctg ccgcctgaaa atacggttga tgtaccgctt tcggcttgct
     1681 gtgcactcgc cggagtggcg attgcgtcat tattatcgga agaaggaaag aattttaaca
     1741 taacagcaga cacggcaaaa agaagtacgg taagtatcca taatttgcgt cgtacattgt
     1801 acatattaaa aacctccttt cacgtctgct cgtttattta gaataatgat tttataccta
     1861 ttataataag aataattccg ccgagtattt ctgctttgtc gccaagtttc tcacctgctt
     1921 ttgtaccgac aaataatgcg ataagactta gaacgaatgt agttaccgca ataatcggtg
     1981 cggactgcca tatgtttgca ctgtttgcgg caaacgaaac acctatggcg aatgcgtcaa
     2041 tgcttgttgc aactgcttga acaagtaata tccaaagtgt gagctt
//
LOCUS       DAWACS010000244         2082 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5412_length_2082_cov_1.503207, whole genome shotgun sequence.
ACCESSION   DAWACS010000244 DAWACS010000000
VERSION     DAWACS010000244.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5412_length_2082_cov_1.503207"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          609 a          457 c          328 g          688 t
ORIGIN      
        1 acttcaccga gtacggagca tatctctctt tcggacaatg ttctctctgc cgcaagatta
       61 agcaaatctc ttactgtaac gcccttaaaa cgcacaggct gttgaaatgc gtatgcaatg
      121 ccgttttttg cacgttttgt aatatcccaa tcggttatat tctcaccgtc aagcaaaatt
      181 tcacccgaat taggcacttc gagtcctgcg ataatctttg caagagaagt tttaccgcca
      241 ccgttcggac ctgttataac ggtcattttg ccgtcaggta tttctaaatc tatacccttt
      301 aaaattttat ctccatcggg aatttcccaa cttatatttt taagctcaag cacttttcaa
      361 tgctcctttc gactgtttat cattagttat gataacataa tattcattga attataattt
      421 tatcataaaa caataccgtt gtcaatttta tctaacaaaa aatgagcgtt ctgtaaacgc
      481 tcatttaatc attaattaat tactttgttt caagaatttt atcaaggctt acaagtttta
      541 cgcaagtaac gaaaatttca cacgcctgct ttaattcttc tgttgacggg aatgacggtg
      601 caatacgaat atttgaatcg tgagggtctt ttccgtacgg atatgttgca cctgccggag
      661 tcataacaac gcctgcttct ttacacatag cgacaacttt tttagcacag ccgtccatag
      721 tatcgaaacc gataaagtaa ccgccgtttg gctttgtcca ttttgctatg cctaaatctc
      781 caagttcctt gtcaagcatc tcaagcagag cttcaaactt aggtcttata atatctgcgt
      841 gctttttcat atgctcaagc ataccattga agtcaccgaa aaatcttgca tgacgaagtt
      901 ggttaagttt atcatgaccg atagtctgta ccgacatctg ctttttaata aagtcaaggt
      961 tagcctttga cgctgaaata gcggctatac ccgaacccgg gaatgtaacc tttgatgttg
     1021 aacagaactt aaataccata tcaggattac ctgctttttc acattcttca agaatttcaa
     1081 gaatgtggtc ttggtggtcc tcatatagat gatgtatgca gtatgcgtta tcccaataaa
     1141 ttctgaaatc ctcagccgcc ggctttagat ttgcaaaacg tcttactgtt tcatcagaat
     1201 atgttatacc cataggattt gaatacttcg gtacacacca aatacccttg attgcctcat
     1261 cctttgaaac aaggtcctca accatatcca tatcaggtcc gtcctcagtc ataggaatat
     1321 taatcatttc aataccgaaa aattctgtaa ttgcaaaatg tctgtcatat cccggtacag
     1381 gacacaagaa cttaacttta tcaagctttg cccatggagt tgagcccata acaccgtgcg
     1441 tcattgaacg ggcaactgtg tcatacataa cattaagact tgaattaccg taaattataa
     1501 tattgtctac gggacattca atcattgcac ttagaagtct tttagcctca attataccgt
     1561 caagcacacc gtagttacga cagtcaacgc cttgttcacc tacaagattg tagtcggtaa
     1621 gtgtatcaat cattcccata gaaatatcaa gctgttcggt attcggctta cctcttgaca
     1681 tatcaagttt taaaccctta gcctttagct catcatattt tgccgcaact tctttgcgga
     1741 tttcctgtaa ttcttcttta gtcatttctt tgtatgacat caaaaaccct ccttaaaaca
     1801 ttccactttg ttttattata ccacacactg tataatcagt caattaaaaa tgttacaaat
     1861 aacgcaaaaa gcctacttta atatctgtta ctttacaata aatttgtact ctgttgtaaa
     1921 attatattca tcgccgacct ttaaaatagg agcaataaag tgactgttct tcataccgtt
     1981 tgggaaatac tgcgtttcaa gacaaaatgc ctgatgtgta ctgtattttg caccttcttt
     2041 atatatcccc tccggaagtc cgtttgatgt atataactga ac
//
LOCUS       DAWACS010000245         2081 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5415_length_2081_cov_2.287266, whole genome shotgun sequence.
ACCESSION   DAWACS010000245 DAWACS010000000
VERSION     DAWACS010000245.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5415_length_2081_cov_2.287266"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1035..1044
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          698 a          395 c          461 g          517 t
ORIGIN      
        1 gttacacact tgtaacaccg gacaccaatt atgttacaaa taaggagtat ggttttcttg
       61 gtattgacga agattcgtac aaacttggaa atcgcttgga cggcttcggc aaccaaaaag
      121 gacaggttat aaaacttcaa gccgccggcg gtacaggtct taatgacgca atcggttcag
      181 tgggagagga tagcttcgga aatgccggtg acgtatatta tcctacacgt tttgcgttga
      241 aagcagatga tgaggcatat tacagagttc gtgcaacagt aacaactctt gatacaacta
      301 aagatgctga aatttcactt tacacagaaa gaaagcaccc tatattcaca gatacaaagg
      361 ttgaggcagg tcagacaaag acagtcgagt tttctgtaag acctactccg atttactacg
      421 aaaagtctga gcctaagggc actatagcag acggaatggt aaatgtgtgc gtagcgggta
      481 aaaactctgc aatcgcatct atcgaaattc aaaaagtaca agaataccct gtattttggg
      541 tgcttggtga ctcaacagtt acagacggta actgttcatt gccattcttc agacttcaaa
      601 actacacagg tgtaggtaca ggcttgacaa aatatttgcc gagaaattat gcaatggtaa
      661 acgaaggtga aggcggtctt aatgctgccg ataactacca ctttaatatg gtaaaggatc
      721 gtatcaaagc aggcgactac ctatacgttg aatacggtca caaccataag agtgacggac
      781 ctacaggtta tgtatcaaac cttgataagt attacaatgc ttgtcacagt gtaggtgcaa
      841 cgcttgttat cgtcagcccg atcgaaagaa ttaacacatt cacagacggt gcatatcagc
      901 acacattgga cggctttgcg acagccggtg cacagtatgt tgcagataag gtaacagccg
      961 gtgcaacaga tattgcatat gttgatttga attcatacag tttggatttc tataataaga
     1021 ttacaactga taacnnnnnn nnnnctatta agttctattt ccaaacagct aagggtggcg
     1081 gaacagacca aactcaccct aacgatgcag gtgctgaaaa tcttgcatat gaattcgtta
     1141 aggctgcaaa ggctgtaacg gacgaaacac aaaaggctgc tttggcacct gttgtaaata
     1201 actttacaga cgaaacacca aaccttgttt caacagaaat cacaagtctt ggcgctgcac
     1261 caaatgacgc ttggccgcaa tacgttgttc cgacagataa cgaatatcct gttgtaatta
     1321 aagatattaa attcaacgaa gccggtgagg cgaattatgc taaggtgctt gtacaagacg
     1381 caaaaataga ccttggtgct tacggtatta tcgttattac ggttaaagac gagaatggtg
     1441 aggaaaaggg taagatttat gctatagacc aggttgataa ctcaacaggc aacggtacac
     1501 aggaaattac acactttacg acagacgtaa aacttgaaga aggcgataca tacacagcaa
     1561 cagtatggaa agctaaggac aacggcggtg acacaggtct taccgttgac cctgaaaatg
     1621 tacaatattc ggctgaatat attccgacag atgttgaaga acagtatctg ttaaatgaag
     1681 ataaagacgg aaatgaacaa tttggatttt acggcgcaac atatgacggt aaaacatcat
     1741 cattgaaaga atttaatgat tggacacaaa tcggcagtgc cggaataaca gcatatctta
     1801 accaaacaag tgatgaaaca aaatatgttg aaatcacatc agacggtgct aaaaacggca
     1861 gtgccaacca aggttcgttc tactattcaa aagatctttc aactgaaatc ggtacaacag
     1921 gcagatacct tgtttcagct gatgttcagt atgtatcggg cggtggtatg acattcaatc
     1981 ttgtaacggg tcataacgat aaaaatttag gcggtacaga aagccttaac ttgttcacaa
     2041 tcggttcagg cggtttggta actgcgggaa ataaagaagt c
//
LOCUS       DAWACS010000246         2074 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5439_length_2074_cov_2.111441, whole genome shotgun sequence.
ACCESSION   DAWACS010000246 DAWACS010000000
VERSION     DAWACS010000246.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5439_length_2074_cov_2.111441"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          682 a          376 c          431 g          585 t
ORIGIN      
        1 acccggggtc atttccgcaa cagtgataag gtcggaaaat tcctccatag tcataaggtg
       61 gtgtatgttt actatttgtt cctgaataag cggaattgcg gcataaccgc cgccgacgct
      121 gaacagaccg acttgtataa atgaaaaaaa taatttcagt aaaagcatta tctaatctcc
      181 tttttatgtt tgtcttttaa actcgacacc agtaaatcga gtaggcctat tccaagacat
      241 acgagtatga ctataatagc gcttacccct aaaaaataag tacatacaaa cgcaataatc
      301 atcattgcga tatataaaat tctgtgggtt ttgaatacgt ttttcgcaag atttataaca
      361 acgtcaaata taactgccgc aacgcctgca cgcataactt gaagtgcaac ggctatgtat
      421 ttgtttgttc ggaattggtt atagaacaga gatatgattg aaagtacaat cataggaggc
      481 aatattgtgc cgaaaacagc tgaaagcgag cctaaaattc ccgccattct gtaaccgatt
      541 ataacagacg cgttgacggg cagcggaccg ggtgcggact gagttattgc ggtcacgtca
      601 agcatttcgt cctcatcaag ccattgcagc tcctcaacaa attttttctt cattaatgaa
      661 acgattacaa atccgccgcc gaatgtacat gagcttaaaa caaacataga cttaaataat
      721 gtccataata tccacgcttt actttttttc attatgtaaa ttctctccta ttttattttt
      781 aacgattaca ttatatcatt cttttttgtg tttgtaaatt cataggtcag aataagatgt
      841 taataatttt aaaaaaatta taaaaaaata ttgttttttg aaataaaatc tgataaaatg
      901 taaggcggaa ttaattttta aaacaatgga gtgatacaaa tggcaattaa aataggtata
      961 ttgggatacg gcaatcttgg ccgcggtatt gaatgtgcaa tcaagcaaaa cgatgatgtt
     1021 gagcttgctg gtgtattcac aagacgtgat ccgtcaactg ttaagattat gacagagggc
     1081 gttaaggttt attcagttga tgacgctcct aagatgaagg acgaaatcga tgttatgatt
     1141 ctttgcggcg gaagtgcgac agaccttccg aaacaaacac cggaatatgt taagtatttc
     1201 aatgttatag acagttttga tacacacgca aaaattcctg aacattttgc ggcagttgac
     1261 gcagctgcaa aggctaacgg caacgttggt ataatttcag taggttggga cccgggtatg
     1321 ttctcactta accgtcttta tgcaaatgct atactttcag acggtaagga ctatactttc
     1381 tggggcaaag gtgtgagcca aggtcactca gacgctatca gaagaattga cggagttaag
     1441 gacgcaaagc aatatacagt tcctgttgac tctgcactta aggcagtaag aaacggtgaa
     1501 aaccctgaac ttacaactcg tcaaaagcat acgcgtgaat gttatgttgt agcggaagaa
     1561 ggcgcagata aggctcgtat agaaaatgaa atcaagacaa tgcctaacta tttctcagat
     1621 tatgatacaa ctgtaaactt catttcagaa gaagaattaa aggctaacca cagcggtatt
     1681 cctcacggtg gatttgttat cagatgtggt aagacaggtt ggaacggtga aaatagtcac
     1741 attattgaat acagcctaaa gcttgattca aaccctgaat ttacttcatc agtgcttatt
     1801 gcatatgcaa gagccgcata tcgtatgagc aaagagggac aaagcggttg taagactgta
     1861 tttgacgttg caccggctta ccttagcaag ctaagcggag aagaacttcg caagaatctt
     1921 ctataataaa taacacaagc aaaagccgtc ttaggacggc ttttttatct gagagggaat
     1981 atgattttag taatagccga gaaaccgtca ttgggcagag atattgcgga cgcactgccg
     2041 ggacaggtta cagagagaaa taacagattt atac
//
LOCUS       DAWACS010000247         2074 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5442_length_2074_cov_1.953938, whole genome shotgun sequence.
ACCESSION   DAWACS010000247 DAWACS010000000
VERSION     DAWACS010000247.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5442_length_2074_cov_1.953938"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    422..431
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1119..1128
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          540 a          485 c          334 g          695 t
ORIGIN      
        1 tcttcgcaag tgcgtttctt ataatatttg tctgttctct ttgacggaac gggactgtta
       61 tcttttccgt caagtcctaa aagttctcgg catataatag agcctgtttc cgctttaaat
      121 tgatttgcga gttcctgtac aagtgcatag gtatcttttt taccctcata atctttcgcg
      181 tcgtcatatc cgtacaaaat tccgacaacc ataaacattc ccgttacagc accgcaaact
      241 tctctcattc tgcccattcc gccgccgaat gatgacgcaa gtctcagtgc cgtcttttta
      301 tccataccca tcaagtcgcc aaacgctgcc accattgcct gtgagcagtt gtatccctct
      361 ttaaaaagct gtctggcaag ttcttctctt ttgttcatat taatcacctt acgtttttat
      421 tnnnnnnnnn nataccacac ctgtcaacaa aaaactgcga gcaacaatac ccgcagtttt
      481 tcgcattatt cagctgtcaa actttcatat tcagcctgtg taatcggcat tacagtaccg
      541 tgacgatatg taccgtcaaa cgagaagtct gaatctgccg taaattcacc ctttgtaata
      601 tcggttgtta caaaaggctt atagccctta ctctttgaga agtagtcaag aagtaagcac
      661 cattcatttt taccgtttaa tttataaatt gtaggacctt catagcctgt catactaccc
      721 aaattatagt ttgcaacatc tttccaatca ccgcttagag atgtacactc ctgcattgtg
      781 atacttgatt ttgcctcatt ctttgtaaat ctgtaatatg taccctcatg gtcgataata
      841 gttgtatcaa taaccgcatt aggctcttct atatacaatt caggagcaga gaatgtcttg
      901 aagtcctttg tataacttct gtaaattctg tacttttgat agctgtcatc gcttacaacc
      961 gatgcccaga ataccatata ttcatccttt tcagggtcat agcaagcctc cggcgcccat
     1021 gtacaaccgg cattgtcatt attaatcttt acaaggcttg cctcgctcca attaacaagg
     1081 tcactgcttt cccatacaac aatgctcttt gagccttgnn nnnnnnnngc tgtccagtta
     1141 ttctttaagt tatagacgct taggtctgtt gcgataataa agaacttacc gtcctcgccg
     1201 cgaagaatat acgggtcacg aacgccctgt gtaccgacat tacttgtcaa atacggttgt
     1261 ttattattaa gcgttgtcca tgtcttaccg tcttttgata ctgagaaata gatttgttca
     1321 cgagttgcgt cctcttgtgt atcaacgaag tgaacaaaca agtacgcact catatcatca
     1381 aggttcttaa tattaattgt cttttccacc tttttctttt ctgttgttcc gtctgataat
     1441 gttgctgtta ttttatactt acctacttca agattatcaa aactgcctgc cgcatttgtc
     1501 tgctctgttc cgactgccgt accgtctgaa tcagccaatg aaacctttac ggcatactta
     1561 tcttttgtcg aatcacagtc acttgcataa acgattttat ttccgtcggc atatgctttg
     1621 aaactgtcaa taaccgctgt atatcccaat acagttacat caaagtttct tgtaaattca
     1681 acaccgtctt ttgtcatttt tgcagtcaac gtagctgttt ttgtttcgtc acttcttgta
     1741 atcttacctg ccgttgtaac cacagcagcg tctgaagttg accaagtaac accctcttgc
     1801 gtagggattg taatgtcaga tgtaacagct gtcaaatcac ccaaatcaag tgaatttaaa
     1861 ggattttcgt aagcatagtt atagattaca aaatcatcta tataacctgt tgcgaattca
     1921 cctgtacccc agttagcctt accgatatat gcaactgaat ttttgccgag catatccgaa
     1981 atattaactg ttgaattaac actgcttgtt cttgttccgt ttacataaac atccgttacg
     2041 ccgtcggcaa tcgaaatgat tacatcgttc catt
//
LOCUS       DAWACS010000248         2068 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5466_length_2068_cov_1.436165, whole genome shotgun sequence.
ACCESSION   DAWACS010000248 DAWACS010000000
VERSION     DAWACS010000248.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5466_length_2068_cov_1.436165"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1235..1244
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          682 a          359 c          479 g          538 t
ORIGIN      
        1 aaagaccatt ctgatacaga atatacagta tttaaagctg atgatgacgc tgaatatgta
       61 cagacaatag acattgacct tgcaacagtt cctcatacgg ttgcattccc tcacttgcct
      121 gaaaacgcaa gaacaccgga aaactggggc gatgttgaaa ttgaccaggt tgtaataggt
      181 tcatgtacaa acggacgtat ttcggattta cgtcatgcgg ctgaaattct aaagggtaag
      241 cacgttaaaa agggtataag agttatcgta ttccctgcaa cacaggcaat ttatcttgaa
      301 gcattaaaag acggtacatt atcgacactt gttgaggcag gctgtgtaat ttcggcacct
      361 acctgcggac catgtcttgg cggtcatatg ggtattttgg cggaaggcga aagatgtgtt
      421 tctacaacaa acagaaactt tatcggacgt atgggacacg ttgacagtga agtttatctt
      481 gcaagtcctg aagttgcggc cgcaagtgct gtaacgggta agataacagc acctgaagaa
      541 gtaatgtaaa aaataaactt cacaacttaa aattgatttt gagttaagtt tggaacggag
      601 gattaatatg aaagcaaacg gaaaagttat taaatacgga gataacattg atacagacgt
      661 aataattccg gcaagatacc ttaacacaac agaccataag gaacttgcgt cacactgtat
      721 ggaagatata gataaagaat ttgtaaataa agttaaagac ggcgacatta tggtcggtgg
      781 tgcaaacttc ggctgcggtt catcaagaga acacgcacct atcgctataa aagcaagcgg
      841 tatttcatgc gtaatcgcaa aggattttgc aagaattttc tatcgtaacg caatcaatat
      901 cggacttcct attcttgaat gtgccgaggc gtcagaggat atagatgagg gcaatgaagt
      961 agaagttgat ttcgatacag gcgttatcac aaacaagaca aagaataaga cttatcaagc
     1021 agttgcattc cctgaattta tgcagggcat tatcaatgca ggcggtttgg ttgagtacat
     1081 aaagaaagga atgtaataaa atggctgaat ttaatattgc acttataccg ggtgacggaa
     1141 ttggtcctga aatcgtatcg ggtgctgtta cagttcttga cgcggtaagc aaaaagtacg
     1201 gacacaaatt taattatcag gaagtatata tgggnnnnnn nnnngggatt cacagccttc
     1261 ggaaaacaga ccggaaaaag gaattttggg cataagaaag gctcttgaat tatattcaaa
     1321 tcttcgtccg tcaatcctaa agccacagct aaaggacgcg tcaccgctta aagacgaaaa
     1381 gcttgaaaag ggacttaacg taatggttgt ccgcgaactt acaggcggta tttacttcgg
     1441 tgaaagaaag aaagccgagg acggctcttg ggcgtcggat acagaaaagt attcagttga
     1501 agaaatcaag agaataggta agattgcatt tgaaacagct atgcgtagag ataaaagagt
     1561 tgtatctgtt gataaggcca acgtacttga atcttcaaga ctttggagaa agactatgat
     1621 tgagctttcg gcagactatc ccgaagttga gcttactcat atgtatgttg ataatgcagc
     1681 tatgcagctt gcaattaatc cggcacaatt tgacgtaatc gttacatcaa atatattcgg
     1741 agatattctt tccgaccttt caagtgctat tgcaggttca atcggtatgt tgccgtcagc
     1801 gtcacttggc gcaacaaaat gcggtatgta cgaaccgatt cacggcagtg ctcctgatat
     1861 tgcaggtatg gatattgcaa accctattgc aaccattgtt tcagccgcta tgatgcttga
     1921 aatgtcattc ggtcttgtag aagaagcaaa ggcaataaat gacgcagtag ataaggtact
     1981 tgaccaaaat tacagaacaa ccgatattat gtcagacggt atgaaaaagg ttggctgtaa
     2041 ggaaatggct aaacttattg cggaaaat
//
LOCUS       DAWACS010000249         2067 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5468_length_2067_cov_2.002982, whole genome shotgun sequence.
ACCESSION   DAWACS010000249 DAWACS010000000
VERSION     DAWACS010000249.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5468_length_2067_cov_2.002982"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          522 a          491 c          320 g          734 t
ORIGIN      
        1 cctctatgca gtcggcaata tctttctttt ccgttctgtc ctcatgagaa cgctgaaaat
       61 agtaaagcaa atcttcgact gtctttattc ctttttttgc gaataattcg gctcttttct
      121 cgccgacacc tttcaaatat cttatatcct taggcatatt ctgactccat aatattattc
      181 tgcggaaata atatagtagt aaagcggctg accgcctttt ttaaacgaaa cttcgatttc
      241 atcgtccgca tactttgttt caagtgcttt aagcattaca tcagcctgcg gtttcttgat
      301 ttccttaccg taatatacgt tcaaaaattc agtatcttcg tcaaccatct gtgaaactac
      361 ccggttaagt acatcgtcaa tatccttgcc taccgttacg attttaccct caaccattcc
      421 gagaatatca tcttttttaa tttcaatacc gtcgatttcg gtatcacgaa cggcataagt
      481 aagctgaccc gatgtaactc tgccgatagc cttattcata gccttttcat tagcctctgc
      541 cgacttttta tcattaaatt ccaccattgc gcttatacac tgcggaactg actttgacgg
      601 aattacaacg attttcttat cctcaacaag ttctgcggct tgatttgcgg ccataataat
      661 atttttattg ttcgggaaca cataaaccgt ctttgccttg acacgtttta cggctttcaa
      721 aatatcgtcc gtactcggat tcattgtttg tccgccctca ataactctgt caacgccaag
      781 gtctttaaga atatttgtaa ttcccttacc catacatacc gctacaaaac cgtaatcctt
      841 taatacaaca ggtactttcg gtttaggagc ttttttaggc ttttcagtct ttttctccgc
      901 cttgacttcc gctgttttaa cttcggcatt ttccgaagtc tgttcattac ttccgtcaat
      961 aatagacttg tgctgatgtt tcatattatc tattttcaaa ttaatcattt caccgagttt
     1021 tatagcctct tcaagcacaa aacccggatg atttgtatga atatgcacct ttacgatttc
     1081 atcatcgtca attacaagca tacagtcacc ctttggggca atagtatctc tgaacgtact
     1141 tacactaaga cctttttgct ttttctcaac gataaattcc gtacaatatt taaactttat
     1201 atcttctgtc ttaattgcct cttgtgactt tttctttaca ggagcagtct gcgtttcaag
     1261 tgcttcgcct tgtctttcaa caacattacc gctctttaaa taagacagtg caccctcaag
     1321 cacaaacatc cagccttgac cgcctgcgtc aacaacgcct gcctgtctta atgcaggtag
     1381 catttgagtt gtcttttgaa gtgccttgtt acctctttca atagcactct ccattacgtc
     1441 aataatgttt tcgtttgtat ttgcggcaag ctgtgcacct gttgcgactt cacgcgaaac
     1501 agtaagaatt gtaccctctg tcggcttcat taccgcctta tatgccgcat ccgagccgtc
     1561 ttttaaagca actgcaagtt cttccgcatt acattctgtt ttgcctttaa ggctctttga
     1621 tatacctctg aaaaactgtg acaggataac accggagtta cctcttgctc ctcttagagt
     1681 tgcgaatgac attgtatcag ccgcttttgt aagagttgtg tcgccttttt tcaaaagttc
     1741 agtcgccata gccgttgcgg taagcgacat atttgtacct gtatcgccgt ccggtaccgg
     1801 gaaaacgtta agttcatcaa cagtttttct gttattatat aaattatttg ctccgcttat
     1861 aatcatttga gcaaatgtgt ttccgttaag cgtattaatt ttaatcaccc ctaatcagtt
     1921 ttagtcaaca cggacacctt caacacgaac attaattctg ttcactttaa ttccaactgt
     1981 attttcgata gtatatctga ctctgttcac aatactttta catattgtat taatatttat
     2041 gccgtactca acgataagat gtatttc
//
LOCUS       DAWACS010000250         2066 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5473_length_2066_cov_2.090005, whole genome shotgun sequence.
ACCESSION   DAWACS010000250 DAWACS010000000
VERSION     DAWACS010000250.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5473_length_2066_cov_2.090005"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    237..246
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    527..536
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1829..1838
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          640 a          337 c          523 g          536 t
ORIGIN      
        1 ctataaacaa aggtgaattt gttgcgatag taggtcagtc gggtagtggt aaatctactt
       61 gtatgaatat tataggttgc cttgacgtgg caacaaaggg tgtttacaga ttaaacggac
      121 gagaaattaa taattacagt caagatgaat tggctgatgt aagaaataaa atgcttggat
      181 ttatatttca gcaatataac ttactgccga aattaacagt attggaaaac gttgaannnn
      241 nnnnnngagg aatgtcaggc ggcggaatgt caggcggcgg aatgtccggt ggtggcggaa
      301 tgccaggcgg cggaggaatg tcaggcggcg gcggaatgcc gggcggaaga taaagcggag
      361 gtgaagtaaa atgcctccat tggtagaaat gaaagatgtt tataagattt atcatatggg
      421 tgatacggaa gttcacgcac ttgacggaat atctcttact ataaacaaag gcgaatttgt
      481 tgcgatagta ggtcagtcgg gtagtggtaa atctacttgt atgaatnnnn nnnnnntaag
      541 aaataaaatg cttggtttta tatttcagca atataacctg ttgccgaaat taaccgtatt
      601 ggaaaacgtt gaacttccgc ttttgtatgc gggacttagc gatgcggaac aagagaaaag
      661 agcacttgct tcacttgaca gagtaggact taaatcaaaa gcgaaaaacc acccgacaca
      721 gctttcgggt ggtcagcaac agagagtgtc aatagcaaga gctttagcag gcgatccgtc
      781 ggtaatactt gccgatgagc cgacaggtgc acttgactcg cgtacaggtc gtgaagtaat
      841 gctgtttttg cagaaactta atgcggaggg taatacgatt attcttatca ctcacgataa
      901 ctcgatagcg gcaaaagcaa aacgtatcat tcgtataaca gacggacgta tcgtttatga
      961 cggtccggca gagggtgacg taacagtaat gaaccagcac ggtgccggcg aagatgctcc
     1021 cgaagatgaa agagcaaagg gggcgacttg attatgagta tttggaaatg ttttcagatg
     1081 gcaatgtcaa gtattgtcgg caacaaaatg cgttcaatac ttacaatgct cggtattata
     1141 atcggtatta cggcggttat tgcgctagta agtcttatgt cgggaatgac atcggaaata
     1201 acggacgcat ttgaagaaat gggtattgaa tcaatcaatg tttcaattca agaccgttcc
     1261 gcgacaaagg aagtaaagcc tgaagaaatg gaacagcttg cggaagaaca ttccgatatt
     1321 attgcaggtc agtcaccgac agtttcaatg cctgcattta taaaaaacgg cagtgataca
     1381 atttcaacac aggcaacagg tgtaaacgaa tactatctcg gaatgaaaaa gtataatatt
     1441 acatacggac gtgagcttac atatgttgac gttgagagaa agcagaaagt ctgcgttatc
     1501 ggtacttata tagcaaaaga agttttcggc ggtgacgcac tcggtaagtc tattcaaata
     1561 acgggtacgc cgtatgaagt tgtcggaata cttgaagaac aggacgactc gacagaaaat
     1621 tcttcggata actgtgtgta tataccgtac actttggcgt catacacaaa ccgtacatca
     1681 accgtaagca gctatgttgt ctatgcggtt gaccaggaaa aggttgagga agcggttttg
     1741 cttttagaag cactttgtga taaagatata ggcgacagtg attactatac tgtaacgtca
     1801 atgaagtcta tggcagacag tgtaaccgnn nnnnnnnngt attatgaata ttatgcttgt
     1861 gtcggttacg gaacgtacaa gggaaatagg tatcagaaaa tcgcttggtg caaagcagaa
     1921 agatatactt tctcaattcg ttattgaggc gggtatgctt agttgtttgg gcggtgtaat
     1981 cggtattatt gtaggctcgc ttttggcaat actggcaggt aaacttttgg ggatggatat
     2041 attaccgacc tcgacatcta ttgtag
//
LOCUS       DAWACS010000251         2062 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5489_length_2062_cov_2.188839, whole genome shotgun sequence.
ACCESSION   DAWACS010000251 DAWACS010000000
VERSION     DAWACS010000251.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5489_length_2062_cov_2.188839"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    682..691
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1426..1435
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          568 a          416 c          345 g          713 t
ORIGIN      
        1 cttctgttgc cccgaagtga cgtaaaaggc ttacaattct ctttgaagtc gtacctctta
       61 cgattgagtc gtcaaccata acgacacgct ttccctcaac tgtgcttttc agtacgttaa
      121 gtttaattct tacggaattc tcacgcattg cctgtgacgg ttgaatgaaa gttctgccga
      181 tatatttatt ctttacaaaa ccgatgtcgt aaggaattcc cgattccttt gcataaccca
      241 ttgcggcact tagtcccgaa tccggtacac cgattacaac gtcagcctca acaggaaatt
      301 cctgtgccaa gtatttgcct gcaagcatac gtgaatcgtg tgcagactgt ccttcgataa
      361 tactgtcagg acgtgcaaaa tataagtatt caaagataca cattgtatgt ggctgtttgc
      421 cgacgtgagt tctgatagtg gaaattttac catcttgaac gacaacaatt tcaccgggct
      481 caatgtcgcg gacaaattcc gcacccacac tgtcaagtgc acaagtttcc gaggcgaaaa
      541 caatagcgtc gcccttttta cccatacaga gcggtctgaa accccaaggg tcacgagcgg
      601 caataagttt ttgcggactc ataacgataa gtgaaaacgc acctacaagg tgatttatag
      661 tcttttcaac cgcctcttca annnnnnnnn nattgtgtaa gcaatcattt ctgtgtcggt
      721 tgttgtttgg aagatagcac ctgtaacgct tagttcttgt gccaattcgt ccttgtttac
      781 gaggttgccg ttgtgggcaa tggcgatatt accttttaca tatctaagta caagcggttg
      841 agcattttca cgcatacttt cgcctgttgt cgaatagcga acgtgaccga cagccatttt
      901 accgtcaagt tttgtcaata tttcgtcatt aaatacatcg ttgacaagtc ccatatcctt
      961 gtaatgagta atgtcacgat tgttatttac cgcgataccg cagctttcct gtcctctgtg
     1021 ctgaagtgca taaagtccgt aataggtcgt tcttgcacaa tcaccgtcag gatcataaat
     1081 ggcgaataca ccacattctt caccgattct gttgtcattt tgcataatct ttatcctctc
     1141 ttaaaatacg ctttgtatta aaattttgat acatattata ttatatactg ttttccgata
     1201 aaattcaaca gtttttataa gtgaatttgc atagcgagat taaatattgc tttttcgaca
     1261 aaataaaaaa tctcaagaat atagctcttg agatttttca ggtcattttg tgaatgcttt
     1321 tgtcaataat ttacaacgat attcgaatta aaattattct ccaaacaatt ttttgttttc
     1381 gttatcaatt tcaatctgtc tttgcttttg ttcatccgaa tagttnnnnn nnnnnagtta
     1441 gggtgcatat tatgttcttt ttcaatcttg tcttttaact catctataac ttcggtatct
     1501 ctttcattat cgacttccat agaacttata taagtattga tagtattact cgaacgtgag
     1561 ttttctgatt ctgataggtc gtattttcct tgtcgcatac ctgttaaaac ataatagtct
     1621 tcatcggttg caatgtcgct gtcatctctt gaaaggaata ataaaacttt ttcattaact
     1681 tcaaagtcag gataaccgtc ggaagaaacg attgtgtttt catcttcgcc tttatctatt
     1741 cttactatag cttgattatc gtcacgttcg ccgaaaaggt aattatcaat gtttacaact
     1801 atgtctgtct gaagaatatt tctgaaattt tcaccttttt cattattggg attactccat
     1861 ttactgtcca taatttctga aacagtaccc tctattatta agtctgaacg taaaataagc
     1921 tcactttcag atattaacgg tataataccg tcaacgacta tttcttcttt ttttgcatta
     1981 tttccgcaag atgttgcagt gagcattatt gtgagacaac acattaaact gattatattt
     2041 aaaatagatt ttttcataat aa
//
LOCUS       DAWACS010000252         2055 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5510_length_2055_cov_2.358500, whole genome shotgun sequence.
ACCESSION   DAWACS010000252 DAWACS010000000
VERSION     DAWACS010000252.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5510_length_2055_cov_2.358500"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          648 a          422 c          376 g          609 t
ORIGIN      
        1 gttcttacag gtgtaagttt tgacgataat tcacagggcg ttatgtccta caacagagaa
       61 acaggcgaat tttcatcgta ctttaacgaa aatattcccg gttacttcca cagtacaggc
      121 gatatttttt caagcacatt gtgcggtgca ttggttaaag gttttgatat gggtaaggca
      181 gttaaaatag ccgttgactt cactgttgac tgtatcaagc acactttagg gagcgaaaaa
      241 gagcattggt acgcggttaa attcgaggaa tgtattcccg atttaatcga aatgataaaa
      301 taaagcaata taatagggac actaaaattt agtgtcccct ttattatata ctcatttaca
      361 cttgtaatta gctatatttg tatgttatac tgatattaac aaatatattt tgaaagggag
      421 tttttatatg aaaaaggatt ttgtaaaagg aattgcagtc ggctgcgcat tgtcaatggc
      481 aatcggtggt gcgtccgcgc ttgccgatac gtacagaaaa tttcttgaag tggcttacac
      541 tgacatcaaa gttgtcgtaa atggaaagcc gacaacaccg cgtgacagtg acggcacccc
      601 tgttaagccg tttatctcca gcggcacgac ctatctgccc gtaagatcca tttcaaacgc
      661 tctgggacaa aacgttgatt gggacagtga cacaagcact atatatatcg gcgataaatt
      721 agaaacagac aaagttaata tggcaaaaat cgagccagtt gaaggtaccc atattttaaa
      781 cagttcaagt gctacatttc cccttagaca agttacaatt gttcctgata accgttttgc
      841 tccttatagt agtactttat ttattcttga cggcaaatat tcttctttag aaggcttatt
      901 tgccgtacct gatcgtgata atgatgatga cataaatgct gtacaattca taaatgaaga
      961 taccgatgag gtgcttgcaa cagtttcaaa taaaagactc gaaaaagccg tagatgtcaa
     1021 agtaaatctt ataggtgttg ataagcttcg agttaaagcc ggtcgtattt cagatgataa
     1081 caactttgag tttggttata acggtggcta tttatacaat ctctacttaa caccaataac
     1141 tcaaaaataa ttcaaccaaa aaggacgctg aaatcagcgt ccttttgtat tcaatttctt
     1201 ttgacattta tcacaatagc cgtatatttc aagtttatgt cccgtcactg taaaattatc
     1261 tatatgcggt gaatgtacgg ggcatatatc gacgtaacgc atttccttac agccaagaca
     1321 tattgcataa tgacggtgtt cgccgtttat gagttcataa taaaacttat cactgtcttg
     1381 aattgtatgc ttactaacta tacccttttc acacagcttt tccgcaattc tgtaaacggt
     1441 cgaaagcgac attccgtcaa ccttgtcata tatattttca accgtaagag gtgcgtctgc
     1501 gtccgacagt acctgcataa cgcttatacg ctgttttgta cattttaaac ctacttttga
     1561 aagtatgccg tttatatcct ccatgctctc accttctcct tttacttgta caaaccatta
     1621 ttacaatcaa gaataccaca cccaaaagca caatcgttcc gcccggtctg agatcaagat
     1681 agtacgatat gaatagtccg gcaacagtga aaaattccgc aaacgcgatt gaccaccata
     1741 ttgtctgctt atagcttttc gcaatcatca ttgccgccgc aacaggtata acaagaagtg
     1801 acgaaatcat aagcgcgccg acaattcttg acgcaaccga aaccgtaacc gccgttaata
     1861 acataagtac aagattaata ccgccgacag gcactcccgc aagctttgcc gcctcttcgt
     1921 caaacgtaac gaagaacaat tctttataca ataatatcga agttataata accgctatgc
     1981 cgagtccaat cgttaagtac aattcaaaat cgtttatcgc aactatcgaa ccgaacaaaa
     2041 agctgtttat attgg
//
LOCUS       DAWACS010000253         2051 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5524_length_2051_cov_2.098196, whole genome shotgun sequence.
ACCESSION   DAWACS010000253 DAWACS010000000
VERSION     DAWACS010000253.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5524_length_2051_cov_2.098196"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          704 a          316 c          441 g          590 t
ORIGIN      
        1 attcttcaac gaacagtttt tcaaacacgt attgtaggtt aagttcagca ccccaacccc
       61 aaccttttgc gaaaggcatt gcaatacagt taccgttatt aacctgtgta aactgataag
      121 cgtctgtagg atctacaacg tgaccgcaaa gtacaccagg gaatgcgtta caagcaaggc
      181 aagcaccttc acctgtacca caacctgtga ttacaaggtc agctgcacct gagtttagaa
      241 gtattgcggc aagaataccg atttgtacat atgtaagttg ttctgcatcg tcagctgagt
      301 acataccgta gttaaatact tcgtgaccaa gcggttcaac aactttcttt agtgtttcaa
      361 caataacgcc attctttgca gcttggctgt tttcgttaat taaagcaatt ttcatttgaa
      421 atttaccttc tttcttttat tttacacttc tattatatct aattctcaat aagtttgcaa
      481 tatataaatt aaaaaataat atactattga caaataatat tatacgatat ataatattat
      541 taaattaaaa catattataa ataaaacagt aaaggcggtg aatatatgat atttaatatt
      601 aatgcggcac tgcttgacgc actcgtactt gcgatagtga aaaaagagga cacttacgga
      661 tatgaaataa caagagtttt aaaaaatgaa cttgatgtat cggaatctac tttatatcct
      721 gttcttcgca ggttacaaaa ggacgattgt cttgaaattt atgacaaaga gtattccgga
      781 cgaaacaggc gttattacaa gataaccgac agaggtcgga aacagcttga tatgtaccgt
      841 gacgaatggc agaactacaa agcgactata acaaatatat ttgagggagt gatagatgat
      901 gaataaaacg gaatttcttg cggcgttaag gcgtgagctt ggttttcttc cgaaagatga
      961 gcttgatgac gctattcgtt attatgatga atatatcaat gacgccggag atgacgaaga
     1021 gaaagtgatt gcggaaatgg gtactccgca taaagtggcg gaagagttta aaaatgagta
     1081 ttacgacaga aaaatcgtta atgatgaaag tgcaaaacac aataaccaaa tttcttggaa
     1141 aacagtgctt gtatgtatat tggcggttgg gtttggcgta cctgtattaa atatgataag
     1201 aaacgctctt ccggggataa ccggaattgt aattcttgtt gtggtaatag tattgcttat
     1261 aaaatttctc ggcaacaaga ccaatcaaca aattgctgtt tcaaatgcaa cagttcaaac
     1321 ttgctctgat attgaaaaca tatatcttga cttgaaagca ggaaattttg aattcagaac
     1381 gggtgacagc tttgctgttg atacaatgtc agccggtgaa gtaaaggtta aaagttatat
     1441 tgcaaacaat acttggacag ttgagggttc aaagactatc ggaaataatg aaaacggggt
     1501 tgtttctata ataataccaa aaggatttca cgcaaagaaa gctgttatga aattaaccgc
     1561 cggaaatatt cttgtgtcgg gattgtcagc cgataataca aaagttgaag tcggtatggg
     1621 aaaatgtgaa attaaaaata tgtattctca gcgtttggat atgaagtgcg gaatgggtga
     1681 catagtcggc agttgcagta tgaacggtga cgtgaatgtt gactgcggaa tgggtaatgt
     1741 aactctaacg ctcacaaaca gtgcagacaa ctttaatatg tcggcgacgg cgggtatggg
     1801 atgcgttgat attggcggca cgaaaattca tggtgcagga agtaagttgg ataacaatat
     1861 cggcgcgttg cataatatta atgtaaaatg cggaatgggt aacgtcaaaa ttggatttaa
     1921 tgaaagggat gaggcttaat gactaagaaa gaatttttag acgaaatcaa aaaagaactt
     1981 ggatttatgc cgtatgagga agtaataaaa gccgaaaaat attttgaatc atatttcaac
     2041 ggcagtgagg c
//
LOCUS       DAWACS010000254         2039 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5557_length_2039_cov_1.927419, whole genome shotgun sequence.
ACCESSION   DAWACS010000254 DAWACS010000000
VERSION     DAWACS010000254.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5557_length_2039_cov_1.927419"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    881..890
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          670 a          321 c          526 g          512 t
ORIGIN      
        1 gtataatgat gtgtcaatta tgaattttga aaacggtaag atagttgaag aagaattcag
       61 cggcggtttt accgactatg accttacatc tacaatgaat gcgtaataac ggaggtaaaa
      121 gattatggat aagaatacaa aaattactct tgcggaactt attaagcgta aggagcaagt
      181 gcttgaggca aagaaaaacg taaaaagagc gagagtttat gtaaaaagcc ttggtggcga
      241 gattgttata aaagcaccga caaagtcgct tgcaacagaa tcggcggaaa tggaaaagga
      301 cggtgacgct caccttgttt atgagtgtct tgccgaaccg gatttacatt caaaagaact
      361 tcaggaggca tacggctgta catatcccga agaaatcgta gaaaagattt ttgacgacgg
      421 cgaaatctca ccgattgcaa tggagtgtat gaaacttgcg ggatatatcg acagtgtaaa
      481 acttgttgaa gaagtaaaaa actgatagag gcagatgatg aactctatat gatacatcat
      541 tatctgcaaa gaggaatatt gcccgaaaag gtgcttgcaa gaccggaaat tgaaaaaata
      601 tttttccttg caagtgccaa aaaggcaaat gatgacgagt acgcaaagtg gaaggcgttg
      661 ggaggtgaat aattttgcgg agcaaaatta tagcgagcaa atgccgtgca tttgtgatag
      721 cgtagggggt gaatagtttt gcagaataaa agttcgatag ttctgaatat gaaccttaat
      781 gcgagtggat ttgcccgagg gataaaaagt gtaatcggca gtgtcaaaaa tatgaatgag
      841 tcgatgaaag acgcaacgaa cagcgcctca aagatgtctt nnnnnnnnnn gtatagggag
      901 cagtgccata aaagtcggaa aaggtttagc ggtggcagga gcggccgcgg cgactgccgt
      961 aacggctttg gtttcaaagt ctgtcggtgc atttgccgat tatgaacaac ttacgggcgg
     1021 tgtagaaacg ttgttcggag caagcggaag aagtgttgag gaatatgcac agagtgtcgg
     1081 taaaagtgtt tctgatattc aaggaaaata tgacagtttg atgagtgcgc aaaatgctgt
     1141 attagaaaat gcaaataagg catatatgac tgccggaatg tcggcgaatg aatatatgga
     1201 tactgttacg ggattttcag cgtcattaat atcaagctta ggcggagata caaacaaggc
     1261 ggcggattac gcaaattcgg cattggttga tatgtccgat aatgcaaata aaatgggtac
     1321 ggatatggag tccataaaaa atgcgtatca gggatttgca aaacagaatt atactatgct
     1381 tgacaacttg aagttaggtt acggtggcac acaagctgaa atgaaacgac tgcttggcga
     1441 tgcacagaaa cttaccggac agaagtatga tatttcatca tttgccgata ttacacaggc
     1501 tattcatgca atccaaacgc aaatggatat aacgggaaca acggcaaaag aggcaagcac
     1561 gacaataagc ggatcgtggg ggtcactgaa agcggcgttt gaaaatactc ttgtcggttt
     1621 gacaacaggc ggagaaatgt ttgatcagag tttggatgca ctggttgatt cggctaagac
     1681 gttcgggcag aatgttatac cggcaataac gggtgcgtta agtggcgtag gttcgttaat
     1741 tgagagtttg gctcctgtaa ttgtagcaga acttccgtca atggtatccg atatacttcc
     1801 acaccttgtt tcagccacaa agagtttggt taccggttta atcagccaat tacctgcatt
     1861 gggaaaggct gttttagatg caataccatc aatttttgac ggtatgacag atgtaatcgg
     1921 tgaaagttct gtaggaaagc taaaagggtc gtttgaggga ctgaaaaata ccataactga
     1981 tacattttca aacattggac caatgcttaa agatttctgt gagggaggta tatcaacat
//
LOCUS       DAWACS010000255         2038 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5559_length_2038_cov_2.249622, whole genome shotgun sequence.
ACCESSION   DAWACS010000255 DAWACS010000000
VERSION     DAWACS010000255.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5559_length_2038_cov_2.249622"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    299..308
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          563 a          469 c          323 g          673 t
ORIGIN      
        1 accgagtaca acggctataa gctgcatatc atttcttttt gccgccgcac tcaaacaaca
       61 tagtgccttt gatgttgagc ctgttttcag tccgtttgca ccgtcataaa aacgtatcaa
      121 tttatttgtg ttcgcaagct ggaactttcc accgcgaagt gtgtccatcc atattgacgt
      181 gtaattgaaa atcgtttcat gtttcatcaa ttcccttgac attatcgcaa catctcttgc
      241 acttgaataa tggtcatctt catcaagtcc gtttgtgttc ataaaatgag tattttccnn
      301 nnnnnnnntt tccataccga gttccttcgc cttttcattc atcatatcca caaacgcact
      361 ttcactgcct gcaagatgtt ctgccattgc cacacaaccg tcattcgcac ttgcaaccgc
      421 aatacctttc agcatatcat ttacagtaag ttgttctcct gtttccaaaa acattgtact
      481 tccgccgtaa ctcattgcat tttcacttac agtgaccata tcgtcaaggc ttatctttcc
      541 actgtcaaca gcctccatta taaggagcat agtcataact tttgtaacgc ttgcaatagg
      601 taatctctcg tcgggattgc tttcgtaaag tatattacct gtcgattctt ccataagtat
      661 tgctgatttt gcattaagtc ctaaatcggt attgccttcg gcaaatacaa caacactatg
      721 catcataaaa catacgcaaa taaaaaagca tattactttc ttcatactta tatcctccgt
      781 aaagtttttt tctgtatgaa tttataatat gctttttatg ggtttctatt cattttataa
      841 gtaaaatgtg aaaatctact atttattttt atgttttata tgtatttccg gcactgccac
      901 gttgtcatat gtaatggttt ctgtatcgtc cgtatcggtt gtttcttcta catcttcttt
      961 gtcgatgtga acttccggta ctgcaagatt ttctgtttca actgttataa atggtttctt
     1021 tttcataata aatccctcca tatcactctt agaacaaatt tataaagaaa gttattgtta
     1081 aactatttaa aaagtctgca aacatactgc ctataatcgg tacaagcaag tatgccttta
     1141 ctgacggcac atatttttct gtaagtgcct gcatatttgc cattgcatta ggtgttgcgc
     1201 ccattccgaa accgcaagta cctgccgcaa gcactgccga gtcataattt ctgcccatta
     1261 cgttaaatag aacgaaatac gtaaatacaa acatcaacgc aacttgtcct gcaagaagta
     1321 tcaatagcgg aagtgccaat gatgcaagtt gccataattt caatgtaatc attgctatgc
     1381 ccaagaataa tgaaagacat ataccaccta tatcatttat ttcacccata tttacggtga
     1441 agaggtttgt gtgttcgctt atgtttcgca ttgccgccgc aactatcatt gcacctatgt
     1501 atattggaaa ggtcattcct gttttgctca gtgcccacga aacaatcgtt cccagtccca
     1561 ttgctatggc aatttgatat gttgcaggtg catacatact tacacttctc ttgtgtttct
     1621 tttcatcctc aacaagtgtt gcatcatctt ccataacggc agtttttaaa aggtcgtgct
     1681 ttaatataag ccgtctgcca ataggaccgc ccataagtga gccggctact aatccgaatg
     1741 ttgccgccgc tgtacaaagt gttgacgcac ccgagattcc taagtcctca agtacaggtc
     1801 cgaatgctcc ggctgtaccg tgtccgccga ccataggtat tgagcctgtg ctaagtccta
     1861 taagcggact tactccgacc aactttgcca accctaccga aacaaggttt tggcttataa
     1921 taagcactat tacgcaaact aaaaatacta caaggcttag tccgccactt ttcaatactt
     1981 tcaaattagc ttgaaatcct accgacgtaa aaaatattac catacataca tttttaag
//
LOCUS       DAWACS010000256         2035 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5573_length_2035_cov_2.413636, whole genome shotgun sequence.
ACCESSION   DAWACS010000256 DAWACS010000000
VERSION     DAWACS010000256.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5573_length_2035_cov_2.413636"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    213..251
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          663 a          379 c          404 g          550 t
ORIGIN      
        1 ccgcaacatt tctcattgag gcgttttcgg gatttgaaaa ttttcggctg aatgtcgttt
       61 ctttcccctc atagaaaata ataactccgc acgcatattc ttttgtggag atgttgtagc
      121 ttccgtcaac atatgcaacg gcggtgcttt ttctttcttt cggttcatcg aataaactga
      181 tttgttcgtt ttcgcctaaa aaagcttttg cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn ncttttatat tccgcacccg aaaaaccgga aacctccttt ttacattcgt
      301 cccaagtgtt aaaaattcct gtatgatagc cgtttctgac ggcataatat ttagccatat
      361 gtgtagtcct ttcagtttgt ttacactatt atattaagaa aaaataatga tttcaataaa
      421 ttttttaaaa ttttttcaaa aaaagcggaa ctttttcgga aaacatacgt ctaatctatt
      481 gtaaaacata aaagcattgg ttgacataat caatttgacc aaaaaatccg tgaattggac
      541 atagattttg tgctaattat aaaattttgt gaaaaagatc gggttggcag aaaaaagtag
      601 ttgacaacag ctttatgaaa gagtaaaata ttcagtagca gttttcgaat aatagggtag
      661 aagcgttagt tgaaaattgt taccgtaata tgagtcacaa aacggcaaag caggttgtag
      721 ccgagatgtg acaagaatta cagttttttt acaactgtaa tacgtcattg acttatacac
      781 agtttgcgtg gtataatcaa tgtgtaatgt agttttgtaa tgcaaagaag ggtgcataca
      841 aaacatctaa ggaaaccctt cacaaacaaa gaatcaaaaa gggaggattt gagactatga
      901 ataagaatct caaaaaggta atttcatcag ttgctgctct tactatggta gcatcttcag
      961 ttgccgcttt cgcagttgat ttccctgacg tagaatctac agcaagctac gcacaggctg
     1021 ttcaagaact ttcagctctt gacgtaatca gcggttatga tgacggcaca ttcggtccgg
     1081 ataagcttgt aacaagagct gaaatcacaa agatgatcgt tgacgctctt gcagaacgtt
     1141 catcagcaga ggcatcaaca gaatcaacaa agttcgctga tgtttcagca gatcactggg
     1201 ctaaaggcta catcaaccaa ggtgttgctg acggtttcat cgcaggtatg agcgatacag
     1261 aatttgatcc tgacgctaac gttacatatg tacaggctca aaagatgctt gtaagtgcta
     1321 tcggttacga aacatttgca caaggtcaag gcggatggcc aacaggctac aagacatacg
     1381 ctgcatcact tgatatcaca aagggtatct caggtatcaa agacagcaca gaacttacaa
     1441 gagctcaggt tgctcaaatg atcgacaacg ctatggacgc tcctctttgc gtaattgcag
     1501 gctggaagcc agagtggaac ggtacacaga cacctaatct tgaagtaaga gacggtaaag
     1561 aaggcagagc atacgaaact ctattcacag aaaagcatga cgcatacaaa gtatacggtc
     1621 gtgtaacaga aacaagcaag actggttcag ttgataacga caaggttaca ttccaagttg
     1681 aaaaagctga caacttcgac gatgaagaag ttaaggcaga ttcacctgtt tcagaagata
     1741 tgtacatcgg cgattcaaag gctgataact acctaagaac atattctcag gcacttatcc
     1801 aaaagaatga cgatgatgaa ttcacaattc tttcaatcgc tgctgctgct gcaaacaaga
     1861 gcgtaacagt tgcttcagaa gactttgatg aaaacaagtc aacagacgaa gctctttact
     1921 tcttccctgc aggcacaaca aagggttcaa caaagtatca acttgataca acaaacggtg
     1981 ttacaatcta tgtaaacggc gttaagcaag atagcatggc tatctatgat gcaaa
//
LOCUS       DAWACS010000257         2033 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5585_length_2033_cov_2.286148, whole genome shotgun sequence.
ACCESSION   DAWACS010000257 DAWACS010000000
VERSION     DAWACS010000257.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5585_length_2033_cov_2.286148"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          623 a          316 c          437 g          657 t
ORIGIN      
        1 gggctgatag ataacacccg aaattgtgtt aggaaatgac gaatgacgta cacgatttaa
       61 aataactgcc gcaacggcaa cttgtccttc gtaaggctcg ccgcgtgcct cgccgtttac
      121 cgctcttgcc aaaagctgtt tttcgtttga tgaggcactt accgcatatg cgttgttttc
      181 gttagtgtaa taaacgacgc cgcaagttat caatgcaaat gccagaacga cagatattaa
      241 ttttttcata ttacttcctc cttgtgattt ttctttagta tttccataaa atcgtaataa
      301 atatgtatgc gagatgaaaa ataggttaat aatttcaata caagattaaa tcagtgaggt
      361 atgaattatg aataagataa tagctgtatt tttggtggca ctttgtatga cggtttgtgt
      421 aactgcatat tcggacggtg tacagtctga tttgcaggat aatttgataa gattacatat
      481 tatcgcggac agtgacgatg ataacgacca agcagttaaa ttaaaggtgc gtgacgcaat
      541 tttaaaaaat gtcggcgaca gactttcaac aacggataaa aacgaatgtc gcgatgagat
      601 aataaacgat ttgaatgaat ttgaagatat agcgaatgac gtacttcgtg aaaacggttt
      661 ttcatatacg gcacacgccg aatacggtaa atttgaattt cccgaaaaaa catataagtc
      721 aatggttttg ccggcgggcg aatattacgg cgtaaggatt gttctcggca gcggtgaagg
      781 acataattgg tggtgcgtta tgtatccgcc gttgtgcttt aaagagggtg aggaagttcg
      841 gctcagtaaa gaaagcgaaa aaattcttcg cgaaaaactt gataaagata cttacgatat
      901 aattaccaaa aacgataaag aagttgttgt taaatttaag ttggttgaaa tagttcagga
      961 aataaagcaa aagataaacg gtgattaaaa atgattttca tttttaatca ccgtttttgt
     1021 gatttgatta aattatgata gtaagtttac tttcagaatg tcttatttca ttctgctttc
     1081 gtaatcagct atcatctttt taaccatttg accgccgata cttccggctt gctttgctgt
     1141 aaggtcaccg ttgtaacctt gctttaggtt tacgcctact tcgttagctg cttccatttt
     1201 gaactgttcc atagcttgct ttgcctcagg aacttgaatc ttgtttttac tcattttgtt
     1261 tccctcctta aaaccaaagt cttaatctat ttaaaatttt gtgtcgaatt gtatcgacta
     1321 cactaatatt ttgtacattg atgaaaatta tattcaaaaa ttttattagt aatatttaga
     1381 atataaatat gttgaaaaaa acggcgtaaa atgatataat ttaatatatt ataaaaagaa
     1441 acagggagat aaatatgata aaggatatag cgattgaaaa aatgaaatct gcgtcaaatt
     1501 acgttattac gtttttgaaa tgggcgttga tttcaataat aatcggagtg gtcggtgggt
     1561 gcgtcggcgc gttgtttcat atgagtgtaa attatgcgaa tattattttt acaatgcacg
     1621 attggtttgt actgtttctt ccgataggcg gtattataat cgtggcaatg tacaaagcgt
     1681 gtaaaatgct tgaaaacaag gggacggaca gtattatttc gtcaataaga aatgatgaaa
     1741 aagtacctat ttcacttgca ccgcttatat ttgtaagtac ggttataact catctttgcg
     1801 gcggcagtgc cggcagagaa ggtgcggcgc ttcagcttgg cggaagtata ggaagcaata
     1861 tcggcaggct ttttaagctt gacgaaaaag atatgcatat ggttgtaatg tgcggaatga
     1921 gtgcggtgtt ttcggctctg ttcggtacac cgataacggc ggctgtgttt gcactcggtg
     1981 taacaagcgt gggaattatg tattattcgg cacttgtacc gtgtcttatg tcg
//
LOCUS       DAWACS010000258         2030 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5588_length_2030_cov_2.442532, whole genome shotgun sequence.
ACCESSION   DAWACS010000258 DAWACS010000000
VERSION     DAWACS010000258.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5588_length_2030_cov_2.442532"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    211..220
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          625 a          314 c          444 g          637 t
ORIGIN      
        1 cgaacagcgg aacggtgctt gataatgtga caatgcacac gattgagcat atgcttgcaa
       61 tgttcgtaag aaattctgat ataaagaatg aagtgttata tttcggtcct atgggctgtc
      121 aaacaggttt ttacttgctt gtacgcgatg tggttccgaa agaaaaagtt cttgaaacgg
      181 taaaagatat tttggcaaag acaattaaac nnnnnnnnnn acgacggcaa agttttcgga
      241 gcaagtgaga ttgagtgcgg aaattataaa acacttaatc tggattgtgc aaagaaagcg
      301 tgcagagaat accttgaggc tattcgtgat ataaacgaca taataacgta tgatgatgta
      361 aaccaaaggg gatgctgatg aagtattcct tttttatttg aacacggtag gagtgaattt
      421 ttatgaaata catttatcaa ttatttataa tattggttgt tacatttgtc ggagaattgt
      481 tgcattattt tatacctatt cccgtacctg cgagcatata cggacttata ataatgctta
      541 tacttttgtg tacaaaggtt gtaaagcttg aacacgttga aagaacttcg gactttctta
      601 ttgaaataat gccgcttatg tttattccgg gcggcgtcgg acttgtaacg gcttggggcg
      661 atttgaaaga tatgatgttg ccggttgtcg ttataacaat catatcgaca atcgtggtta
      721 tggcggttac gggcaaagcg actgaattga ttatgaaaac aggcaagaag aaagagggca
      781 aaaaagatga ataatataat gacagacagc atattcttcg gtgcggtaat cagcgttgtg
      841 ggatatttga taggaatatt tttaagaaag aaaatttcgg ttttaaatcc gttgcttgta
      901 tcaataatct ttgtaatcgc ggttataaag atatttcaca ttgattatga aacgtataac
      961 agcggtgcaa agtatataag ctatctttta acacctgcaa cggtctgcct tgcaataccg
     1021 ctataccgtc aaatatccgt gcttaaagaa cacgcaaagg aagtgtttat aggcatactt
     1081 gcgggagttt tggcaagtat gggaagtata cttttgatgt cgtatttgtt cggacttacg
     1141 catgaacagt atgtaacgct tttgccgaaa tcaatcacaa cggcaatagg aatgggcgtg
     1201 agtgaagaac tcggcggaat acagacaata accgttgcgg taattatcat aacaggcgta
     1261 ctcggtaata taatcgctga ggcggttttg aaaattttca gaataaaaga gcctgttgca
     1321 aaaggtcttg cgataggaac tgcgtcccac gcaatcggta caacaaaggc tcttgaaatc
     1381 ggtgaaatcg agggtgcaat gagcagtctt tcggttgcgg ttgcaggact tatgactgtc
     1441 atatccgcgt caatcttcgc taatatgatg taattcttcg ttaaaagcag taagaattgt
     1501 ttcgtaaacg atattcggct cattcttgaa atacacagcc atacgttctg cctcttcacg
     1561 cgagccggca tatagtgaaa gctttaaaag gttggtgtcg tcatcgtcgt aaagctcaca
     1621 ttcgacgctg tattcgtttt tgcgtatacg ggtaatctta ctgcgtacgg aattacggac
     1681 tttttcggta ttgaaaagct cattgataga attatcaata ggttctttta tgtaaacagg
     1741 tatgtttgga gtgtaaaggt cacttgcatt catacctttt tttgttattt tatatttttc
     1801 gttgtgttca ctttcaagaa aggttttaac gtgatgtgta tgttcgagat tatcaagtgc
     1861 gacttgaaaa tcttgaaaat taatgttaca attatcaaga acaaggttta taagattatt
     1921 gtacggaaca taatcttttg cttgatttaa tgtataaaga ataataaatt gtattaaaac
     1981 gggatcgtta atttcccttt gatacaacat aaaaaatcac ctccgtgtat
//
LOCUS       DAWACS010000259         2030 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5591_length_2030_cov_1.915949, whole genome shotgun sequence.
ACCESSION   DAWACS010000259 DAWACS010000000
VERSION     DAWACS010000259.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5591_length_2030_cov_1.915949"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          714 a          328 c          459 g          529 t
ORIGIN      
        1 attggtaaaa gaccaaactc ctgcacttat tatgcttgat acatcaaaca atatctcggc
       61 tattgaattt aagtgcagtg acaaggataa actaaaaaca gatatagaca atgcacttgg
      121 aaaataatcg aaaagatgag cgatacaacc gtatcgctct tttttgtata ataatctctg
      181 ttttttcaaa agatagatga aaaaaggaga ttgtataaat gcagaatata agaacagact
      241 tggcggtgga ggcacacgaa ctcagcagac gtgaggcgaa aaatgcaacg gaaatagacg
      301 gtgttatatc cgatgtgcgt acagaggacg gaataaccgt tacgaatata gaaataacaa
      361 acgaaaacgg aagtaaggca ctcggcaagg cgataggcaa ttatataacg attgagtcgc
      421 cgaatttgaa atacagtatt gatatatacg aaagggtatg cactcttatt tcggaggaaa
      481 ttcgtaaaat ggccgatata aaaagtgaca gccttacact tgtcgtagga ctcggaaacc
      541 gtgacataac gcctgacgca ctcggtacgg aagtggtgtc aaggctgttg gtgacacatc
      601 atataaaaca gaatatgaaa gatttttttg atgataatat aagcggtgta tgtgcgttga
      661 tacccggagt tctcggaaca acaggcattg aaactgctga gattattaag gcggtaagcg
      721 aaaaggtgaa accgaatctt attatcgccg ttgacgcaat ggcggcggcg gatataagac
      781 gtgtttcgac cactgtacaa atatcggata caggtattca gccgggtgcc ggcgtgggaa
      841 ataacagaga atgtcttaat gaagaaacga ttggtgcaaa ggtgattgca ataggtgtac
      901 cgaccgttat agacgccgca acaatatcaa aggttgaaat accaaaagag cttgcaccgc
      961 ttatggttac gacaaaggac attgaccttg taatcgaaag aacggctaaa accgttgcaa
     1021 acggaattaa tcttgcactg caccgagata tgacattaag ggatatagaa agctatgtct
     1081 aagtccgttg acagttctaa aaatcacatc atagatgtaa tttttgagga ttttcgatga
     1141 ttgacatatg gtttgttttg gggtaaaata tattaaaaac agtaaaggag aaacggaatg
     1201 gaaagtatta atataaattc aaatataaat ctgtattata tacctatgac gaaattgaaa
     1261 acaactacga ttgacgtata tattcacaga gatttaaacg aagaagaatc gtcgcttaac
     1321 gcacttttgc cttatgtgct taaaagaggt tgcagacttt gcagtgacag tgaggcaatt
     1381 tcaaagtatc ttgaaaatct gcacggtgcc ggagtcggtg tcggagtcct aaaacgcggt
     1441 gacgaccata ttttgcacgt tgcatttgaa acaatttccg ataaatatgc accgaacggt
     1501 gaaaaacttt gtgacagcgt tattgacctt atgctgtcaa taatgtttga atcaacttca
     1561 ttcagcgatg aaattgtcga acaggaaaag aaaaacgcaa aagacagaat aatggcaatg
     1621 atgaacgata agcgtcagta tgctgtagaa cgctgtatcg aaacaatgtt tgagggtgaa
     1681 aattatgcaa tttcaaaact cggtacacaa gagggtattg ataaaataac tgccgacaat
     1741 ttgaaaaagc attatgagaa tattattaca tcatctgtta ttgatattta tatatgcggt
     1801 gatgcggata ttcctaagac ggaaatatat gttccgaaga acgaagttaa aaacgttact
     1861 gataaaatgg acgttgtaca aggcaaactt tgtatgggat tttcaacagg cataaagcct
     1921 acagacagtg attatcccgc attgatggtt gcaaacagta tattcggcgg cggcgctcac
     1981 tcgaagttgt ttaacaatgt gcgtgaaaag cttagcttgt gctattatgc
//
LOCUS       DAWACS010000260         2026 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5599_length_2026_cov_2.424150, whole genome shotgun sequence.
ACCESSION   DAWACS010000260 DAWACS010000000
VERSION     DAWACS010000260.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5599_length_2026_cov_2.424150"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1804..1813
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          492 a          468 c          334 g          722 t
ORIGIN      
        1 gttatatcat ccttagtcgg tttatgacgg ctgtcaacaa gcaatatcgt ctgcacaagc
       61 ggttcacggt ttgcaagata atcctccatc atcttgcccc acttttccgt ttcggacttt
      121 gaacgctgtg catatccgta acccggcaag tcaacaagat aaattgtatc gtcaatatta
      181 taaaaattaa tcgtaattgt tttacccggt gtacccgata ctctcgccag cgattttctg
      241 ttaagcaatt tatttatcat cgaagattta ccgacatttg aacgtccggc aaatgcaaat
      301 tccatataac ctgtcgttgg gtattggttt ggacgtactg cggaaattgt aaggctaaca
      361 ttatgtatat tcataattta tatcattcct ttcttcactt atcttaaagc attttcaagt
      421 acagtatcca tactctttac aggtatgaaa ttgattttgc ttcttacttc cttagggaca
      481 tcgtctatat cggctttgtt ttcgttcgga ataataatat cggttattcc tgctctgaat
      541 gccgcaagcg acttttcttt caaaccgcct atcggaagaa ctctgcctct aagcgtgatt
      601 tcacctgtca ttgcaatatc gcttcttaca ggattttttg taagcgccga tacaactgct
      661 gttgccattg ttattccggc tgacggaccg tctttcggta ctgcgccttc cggcacgtga
      721 atatgtatat cctttgtttt atggaaattt tccgcaatac cgagtttttg tgaatttgct
      781 cttatgtagc tgattgccgc cattgcactt tctttcataa catcgccgag cttacctgta
      841 agttcgactt ttccgcttcc cggcataacg ttaacttcta tcgacaacgt gtcaccgcct
      901 accgacgtcc aagccaatcc tcttgcaaca cctatttcgt cctgctcatt cattttttca
      961 aaatggaaac gttccttacc gagatattcc tcaagatttt gctttgtaac ctctatcggt
     1021 ctttcggtgt tttcaagaag tttcttaacc gccttacggc atatcttacc tatttgctgt
     1081 tcaagttttc tgacgcccgc ctcacgggtg taatattcga ttacagcctc aatcgcgtca
     1141 tctgaaattg tcaaatcatt gtcatcaagt ccattatttt tcatagattt cttcacaaga
     1201 taatcctttg caatatggaa tttttcattg cttgtataac ccgaaagttc aatgatttcc
     1261 attctgtcaa gcaacggtct tgaaaccgta tcaagtgaat ttgctgttgt gataaacaac
     1321 acctgcgaca aatcaaacgg tacttcaata taatggtcgc ggaatgttcc gttttgttca
     1381 tagtcaagca cttccaaaag tgcggctgac ggatcacctt tataatccgc acccatttta
     1441 tcaatttcgt caagcaaaat aagcggattt tttgtttttg cctctttcat tgccgctata
     1501 attctgccct ccatagcgcc tatgtaagtc tttctgtgac ctcttatatc tgattcgtcg
     1561 tgaataccgc cgagagatat tctgacatat tttctgccga gcgaacgtgc gattgacttt
     1621 gcaaccgacg ttttacccac tcccggaggt cccgcaaaac aaagtatcgt accgtttttg
     1681 ccttttgtgt tctgtcttac cgcaagatat tcaagcacgc gttccttgac cttttcaagt
     1741 ccgtaatgat cctcatttaa tatcttttca gcatttttaa tatcaaagct ttcctttgac
     1801 agtnnnnnnn nnncctgtat cacttttaat tgctcacgta aataatattc tctttgattt
     1861 ttgtcaattc tttctttgac tttctgtgca agctgttgct ccaacttcaa tacttcaagc
     1921 tgattttgta ttgtaattat aagtttttcc gctctgtcat atgaatcaaa ttcagataaa
     1981 atttcctgtt tttcttttgt gtcaatttca agacttgcgg caatca
//
LOCUS       DAWACS010000261         2023 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5614_length_2023_cov_3.746951, whole genome shotgun sequence.
ACCESSION   DAWACS010000261 DAWACS010000000
VERSION     DAWACS010000261.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5614_length_2023_cov_3.746951"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          702 a          357 c          383 g          581 t
ORIGIN      
        1 tacttgggcg ttgcaaagaa ctgtgcccat aacaagcgcc gtcatcataa caagtatttt
       61 tgtattgaat ttatgtttaa aaaccgacac aactgtcaat accattataa gggacaaaag
      121 aaaaattaat gcaaacgcaa tgtctatgcg ttcgtaaata tacaccccaa tcgaaaagaa
      181 tacggcgatt ttaatatatt caaccatatt tcttactcct tgtatgcttt aattatatca
      241 aatcgcagtt ttctttacaa cggcaatgta acttttgtaa tctttgtaac gcgtttgtaa
      301 tataaatgca ccgtttttta caaagaaaca tactttgcgc gtaatctgcc tgtaatatta
      361 ggctgaaatt ctgtaatttt tgaattattt gaattttgta cacttttaca ttatttcacg
      421 tgtaaaacac gctgtttatg cgacacattg aacattttac cccttgtttt atgttacaaa
      481 agctactgtt ttattactga attgttacaa aaagaatacg atacgattaa aaaagtgttg
      541 acaaatggtt taatgtggtg tataatcctg tttgtaatgt tttagaaata tattagaaat
      601 acaaatgcaa tttaagtaat cgttaagaaa acttaaatca ttaggaggtt ttattttatg
      661 aagaacttta agcattcact tattgctgta atagcagtag caatgataag ttcacttgca
      721 gtagcacaag catccggctt aaacaattca aaaacagaaa gagaacaaag aataaatgcg
      781 gcagcagcag agcagaacat aacaatcgct caaaaggata ttacagacgc agttaaaggt
      841 actgccgaga tttcaacaga acctacagaa acttctgcgg aaaatacagc agaagaaagc
      901 gaaattgcgg caaagagttt ttaccctctt ggctatgtgc ttatctcaaa cggttcactt
      961 gacgtaagag aaactccgtc agacgaggct aacgttatcg acaaagtcga agtatgtaca
     1021 gaagttgaag ttatggaaaa ctcagagggt tggtacaaaa tagcttataa cggcggtaag
     1081 acaggttatg ttccaagtga atcaataact caagataaaa accttgcaga tgaggctaaa
     1141 aagagttata ctaattacag aattgctcaa gttacacttt caggcgaatc tgtaagagta
     1201 agaagtgcag caaacactga cgcagatgtt atagaagaac tttcagacgg tacttatgta
     1261 tatgtactaa gcggtgaaaa tgattttata aagatttgct acggtgatga ctataaagaa
     1321 ggttacgtta tcaatacttc ccttcaattc acaggcgaat ggatagaaaa agacactatc
     1381 gcacaaaaac aacaggaaat tgcagacgca aaggcagccg ctgaaagagc agaaagaatt
     1441 gccaagcaaa acgcagccgc agccaacgtt gcaacatctg caccgtcagc atcttcacaa
     1501 tcagaagaaa cacagtcggc tccgtcatct tcatcaaagg gacaatctat cgtaaacaca
     1561 gcaatgcagt atcttggtgt tccgtatgtt tggggcggta cttccccatc aggtttcgac
     1621 tgcagcggtc ttgtgcagta tgtatgttca aagaacggta tcagcgtaag ccgtgtagcg
     1681 gcagaccaaa gaaataacgg aacttatgta agtcgtgaca acctacaacc gggtgacctt
     1741 gtattcttcg gtaagggcgg aaacattcac cacgttggta tctatgtagg taacggaaat
     1801 atgattcacg ctcctcaaac aggtgacgtt gttaagattt cttcaatcaa cacagaatac
     1861 cgtatttctc aatacgcagg tgcagtaaga gtttactaat tcgatttaat tatactgtcc
     1921 tctcaaaatg agaggacagt atttttttgt gttattttac agttaattat tgacataaat
     1981 ttgttcgata tgatataatt cgattataaa tattgtaaat tac
//
LOCUS       DAWACS010000262         2022 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5619_length_2022_cov_1.694967, whole genome shotgun sequence.
ACCESSION   DAWACS010000262 DAWACS010000000
VERSION     DAWACS010000262.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5619_length_2022_cov_1.694967"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          625 a          440 c          355 g          602 t
ORIGIN      
        1 gtgttcgcct gttgtgtata ttttttcact gtcacggtat atatcaaatg cttgatttgt
       61 aagactttcc gtgcccaaaa gtcgccacga caaatacaca ccgccatttg ttttaacggc
      121 aacagtgcct cgatcaagtt tttccattac tcgtttttca gtagttgaat ttgtgtcggc
      181 atatgacgtc aacaccggta ttgacgcaac tgatgtaacc attacagctg ccgtcaaaaa
      241 tgcggttaat ttttttaaca tagttttttc tcccttactc caaatggctc caatatctct
      301 cttgagcctc tttttttatt ataaacttta atgaataaaa attcaagaag agaatgaaat
      361 atttgccgat ttttataaag aaaaaagctg aatttgaata tatccaaatt cagcttttat
      421 gctaatttcc gcattcaata cttatttcgt taaacactgt caaatagtca tttattgtct
      481 tattatcttt ttccgcacct gaaacatcca taatagcctc gccgttatgc atcataacag
      541 ttcttgtgcc atgctcaacc gcgtgacgaa gattatgcgt taccataatg gttgttatgt
      601 tcttttcatg aacaatctta tccgtaagca tcataaccgt atcagaagat ttagggtcaa
      661 gcgcggctgt atgttcgtcc aaaagcaaca aatcaggtct gatcatagtc gccataataa
      721 gtgcaagtgc ctgtctttga ccgcccgaaa gtgagcctgc aagagttgaa agtctgtttt
      781 caagtcccat acccaaaagt tcaagctgtg aacggtaaaa gtcttttctc tgacggttaa
      841 gtccaaacga aagtccgaaa ttcttaccct tattatctgc gatagacagg ttttcccata
      901 tagtcattga cggacaggtt cccattgcgg gattttgaaa aactctgcct attctctgtg
      961 cacgtttaaa gtttttcagc ttattaagct ccttaccgtc aagcaaaaca cttccgctgt
     1021 caatcggcat atcgcccgaa atgacattta acatagtcgt ttttcccgaa ccgtttgaac
     1081 cgataactcc gacaaaatca ccgtcgttta ctgttatgtt aaaatccgaa aaaagcacct
     1141 tttcatcaat agttccttga ttaaaagttt tataaatgtg agaaagttca agcatttttc
     1201 ttcaccccaa ctttcttctt tttaagcaaa aattcgtcta cgcaaagtgt tgcgataaac
     1261 aaaatcgtaa tcataaggtt tgtatactgt gaacgcaagc ctgcggcaag tgcgattgaa
     1321 atacaagcct tataaataat cataccgaaa agcacgcctg ttgtaattct aaggaattta
     1381 attttcttaa atatagtcga gccgataata acggcggcaa gacccataac aacagtaccc
     1441 gtaccagacg caacgttaaa cgctttgccg tattggcagt acaccgcacc tgcaagcgaa
     1501 acaagtccgt ttgcaatcgc aagaccgata attttaatcg ttcccggatt ttgtgcaagc
     1561 gtgattacaa gcccctcatt atctccgaca cttcttaaaa gaaatcccga cttggtacgc
     1621 atataccaat caagtgcaaa ctttgcgata agcgtaatca caagaataac aacaagttta
     1681 gagtacaatg caaagtgacc gtttccgaaa agaaaagctg tctgtgagaa aatagtcgtt
     1741 gtatccatac ttaggaaatc cgacgctttt ccgactattc cgtaatttat cgaataaaga
     1801 gccgtcatag taagaatacc gcaaagaaga ttgcgtattc tgagttttac gttgaaaatt
     1861 cccgtaaaca taccggctat gcaccctgcg gcaaatgccg caagcaatgc aagccacgga
     1921 ttaagaccgt tattaatcat cacaaagcat atagctattc cgagagggaa cgtaccgtct
     1981 acagacaaat cggggaaatc aagaatggta taggtgatat ac
//
LOCUS       DAWACS010000263         2018 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5630_length_2018_cov_2.561386, whole genome shotgun sequence.
ACCESSION   DAWACS010000263 DAWACS010000000
VERSION     DAWACS010000263.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5630_length_2018_cov_2.561386"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          518 a          493 c          343 g          664 t
ORIGIN      
        1 cttttgtgtc cctcaaggtg caatgtttcg tatttgtcgc tgttatacgt ttcttggaat
       61 accgtcacaa aatctgcacc gcacttgtgc agatgtgcgt attcgtcaga gttcatcgga
      121 taaacctcaa caccgacaac cttaaaatat ttcttcgcta ttttacaggc attgccgata
      181 tattcgacgt ctgacatttt tcggctttca cctgtcaaaa gtaaaatttc ctgtaagcct
      241 gttttcgcga tttcctgcat ttccttttca atttcttctt ccgaaagttt tgctcggcga
      301 attttgttat agcagttaaa accgcagtaa atacaatagt tttcacagta atttgctatg
      361 tacaaaggcg taaacatcga aatagaattt ccgaaatgtt ttcttgtttc aatttgtgct
      421 ctttgtgcca tttcttcaag gtggttaagt gccgccggtg aaagaagtgc cgcgaagtct
      481 tccgttgtaa gtacatcgtg agaaagtgca ttttccacgt ctttatccgt atatttatca
      541 tagtcatacg cgtccatagc cgatatgact tcatcaagca gagtagagtc gataacctcc
      601 ataccctcca tatattccat atgatttgtt ctttctttca ttttcgtaac ctttcaaatt
      661 taatcgtgta aaaatcccgt aagcggcgat gacgctctcg cacccttttc aaccacatca
      721 ccaaagcctg caagatacga ttcacgtcct gcctctattg cttttttgaa tgcctgtgcc
      781 attttcggca cgtcaccggc tgtcgcaatc gccgtatttg ccataactgc cgccgcgccc
      841 atttccattg cctcacaagc ctgtgacggt ctgcctatac ctgcatcaac aataatcggc
      901 aaatcgattt cgtcaataag aatttttata aattcctttg tgcaaagacc tttgttggac
      961 cctatcggtg agcctaacgg cattatacaa gccgcacccg cattttgtaa atctctcgca
     1021 aaattcaaat cgggatacat atacggcatt acaacaaatc cttctttcgc aagaatttca
     1081 gtcgctttca aagtttcgta attatcaggc aaaagatatt tagtgtccct tatcgcctca
     1141 atttttataa agtcaccgca gcctgtttct cttgcaagtc ttgcaattct tactgcctca
     1201 tcggcatttc tcgcaccgga ggtattcggc aaaagcgtta cgccctttgg tatgtaatca
     1261 agtatattcg caacgtcacc gcctgcggca cgtcttaggg caagagttat aatctccgct
     1321 ccggcatttt caaccgccgc ttttataagt tcaagcgaat actttcccga gcccaaaata
     1381 aatcgtgatg taaattcgtg tccgtttaaa attaatttat cttctttgtt catttattaa
     1441 acctctttct ctcctattaa aagtcttaaa atcatatttg cctcatgtgc ggcacatatt
     1501 gccactctcg gtgcgacaag tgcaagtccg tcattcatat cactttcagc gtccccgcat
     1561 aaataaaatc tgtcgtttat ttttcgcgta acaatcgtgt ttgaactgcc gaaacccgcc
     1621 ataccggaac ccgatacaat tttagtgtcc ccacattcag acaagatact gtttacaagc
     1681 attgctttgt tttcagcctt atcaaaagcc tcacacacta ttttatatcc gccgaatatt
     1741 tcagcggcat tgtcttcgtt tactttaacg cattgcgacg ttatttcgat aaacggatta
     1801 aaccttttaa tattctttgt aagcgcgtct gttttcagtg ttcccaaatc tgctatatcg
     1861 tactgttgcc tgttcaggtt tgtaatatcc actttgtcaa aatccacaag gtgcaaatgt
     1921 cccacaccgg ctcttgcaag atacattgca atatttgagc cgagtccgcc aagaccggct
     1981 attgcaacag acgaatgact taatatattg tgtattct
//
LOCUS       DAWACS010000264         1989 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5723_length_1989_cov_1.609617, whole genome shotgun sequence.
ACCESSION   DAWACS010000264 DAWACS010000000
VERSION     DAWACS010000264.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5723_length_1989_cov_1.609617"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    484..493
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          622 a          437 c          344 g          576 t
ORIGIN      
        1 ggtgtaatgt atttgatagt cgttgttgcg ggtgcgttgt ttatgattaa cggcaaaatc
       61 tcccctgccg accttaccgc atatcttttg tatgtaagca cgctccttgc cactttgcgt
      121 acaatcattc aatttaccga acaattccaa cgcgatatga caggtattga gcgtttcctt
      181 gaaattatgg acgcacctgc cgaaataaca gaaaaatgcc gacgaattga aaaatgttca
      241 aggaaatata acgtttgaaa acgtcggatt tcattacagt gacgatgaca caaacgtgct
      301 gtcaaatata aatcttaaca tcaagaaagg cgacagcgtt gcacttgtcg gtccgtcagg
      361 cggcggaaaa acaacgcttt gtaaccttat cccacgtttt tatgacgtca ctgaaggacg
      421 tattttaatt gacggcaagg atataaagaa cgtcacatta aattcgctcc gaaattcaat
      481 cggnnnnnnn nnngattaag cggtgcaaca cgcgaagatg ttataaacgc cgccaaactt
      541 gcaggcgcac atgaatttat ttcaagcctc gaaaacggat atgacacata tgtcggcgaa
      601 cgtggagtta aactttcggg cggtcaaaag caaagaataa gtatcgcaag agtattctta
      661 aaaaatccgc ctatacttat tcttgacgaa gcgacctccg cacttgataa tgaaagtgaa
      721 aaaatcgtgc aagactcact cgaaaaactt gcaaaaggca gaacagtatt cacaatcgcc
      781 catagactta caacaatccg taacgcaaat atgattcttg tcctcaccga aaacggtatc
      841 gaagaacaag gcacacacca agaattgatt gataaaaaag gattgtacta caacctatac
      901 agtatgtaca cctcactata acaaacaaaa atgcggtctg tatgaccgca tttttttata
      961 gtagtggtgc tactaacctt ataatatgtc ctaaaagctt tgtgaaaata ttaaaatgtt
     1021 taacgtcctc taatgtaatc ttctgtgaca attcaagcgt gtctttgaaa tcacgttcaa
     1081 tatccataac cgccggtaca ttaagcatat aagccgcaca ttcatagtga agatacaaac
     1141 ttctgtaatc gtggtttact gtgccgacaa tggctttttt accgtcactt atcgacattt
     1201 tcgcgtgaac aaaaccgggt gtatattcgt atatttctat acctgctttc aaaagttcct
     1261 tataatatgt tctcgcaaga tagaacgcat atttcttgtc aggaatatgt ggcattataa
     1321 tcttaacgtc aacgcccctt tgagccgcat acttcataca ctcatacatc tcgtcatcaa
     1381 taacaagata cggcgtcatt atataagtat attccgacgc attgttcaga ttatttatat
     1441 atgcacgctt tccgacataa acttcatcaa gcggagtatc gccgaacggt attacaaatc
     1501 cgtccgtact tacggaatat ttttttgacg cttggatata ttcaccgcaa tcctcaatgt
     1561 gttcatcatt tacgttccac atttcaagaa acatcgccgt aaatcccgcg accgcctcgc
     1621 ctctgagcat aaatcccgta tctttccaat gcccgaaacg catcttttca ttaatatatt
     1681 catctgaaat atttatccca ccgcaaaatg ctgtcgcacc gtctataacc attatttttc
     1741 tgtggtcacg attgttttgg tgagttgaca aaagcggtac aatcggcgaa aatatacgac
     1801 atttaatccc ctgtctttca agatattcat tgtattttcg cggaagcgtc acgatacagc
     1861 ccataccgtc atacataacg cgaacttcaa cgccctgttt aacttttttc tttaaaattt
     1921 caagaatttc attccacata cggctcgtgt gatttataat aaagaactcc aaaaatatga
     1981 atttttccg
//
LOCUS       DAWACS010000265         1986 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5735_length_1986_cov_1.897462, whole genome shotgun sequence.
ACCESSION   DAWACS010000265 DAWACS010000000
VERSION     DAWACS010000265.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5735_length_1986_cov_1.897462"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          565 a          455 c          313 g          653 t
ORIGIN      
        1 caaataaacc tgtaataaca agttcgcaag tcacaaaatc aaataataca tatactttca
       61 acgtaaatgt atccgatgta acagcagatt gtgagttaat tgtagttctt tacaaaaacg
      121 gaaaagttgc aaatgtcaaa acaactactt taacatcaaa tacaacaaac aaaactttaa
      181 acttgacggc taccaatgcc gacagtgcaa aaatatttat atgggacagt cttaaaaata
      241 tgataccttt atgcaatgcc gtaacaaaaa atctttaata aacataaaat gcagtgatta
      301 taaaaaatca ctgcatttta atttatatct ctctgtactt tgcctgacct atttcataaa
      361 ggtcattacc ctcgcagtct ataagtacca ctgccgggaa attttcgact tcaagttttc
      421 tgattgcctc agtacccaaa tcgtcatatg cgacaacttc ggcttttttt atcgattgtg
      481 caatcaatgc acccgcaccg ccgattgcac caagatacac aacggtatta cgtttcattg
      541 cgtcaacaac ttctttgctt cttgcgccct tgcctatcat acagccaagt ccgttatcta
      601 aaagcatcgg cgtgtacgcg tccattctgc cggctgtagt cggtccgcaa gagccgataa
      661 cttcgccggg tttagcagga catggacctg cataataaat cacgttgtcg gtcaaatcta
      721 tcgggaacgg tattccgttt ttgaaatttg tcgtcattct ttcgtgtgcc gcgtcacgcg
      781 cggtatatat tgtgcctgtt ataagcacgc tgtcgcctgc tttcaggtct ttaattttat
      841 cctttgtaaa tggtgttgtg agtcgttttt ccatttattc tccctccact aaaaaatctt
      901 ctacactttt taaaatatag ttcgccccaa gtttttcaaa ataacctctt gctctctcgc
      961 cttgtattcc cgtaagcacc gcacaaaagt ctgcgcccat cgcctgtgcc gccagaatat
     1021 ccgcacctgc gtcaccgaca accagtgtag tctttatttt tgctttatca taatcgccgt
     1081 caacaatgcg tttattgctg taatcagtgc cgtaaagcgc ctttaggaac atatatgggt
     1141 gcggctttgt aagtgcattg ttgttaagct ctttttcagc ctcgacaaca ttatcataat
     1201 tgcaaagtcc gttttgtgca aaatactgct ttatccccca attttcaatc ggcggcagca
     1261 tttctatata cggtctgccc gtacctgtac acacgcgttt atttcggctt aaaagactca
     1321 ttattgtaac aagtttattc ttatcgacaa tcggttcttc cttatataaa agccccgttt
     1381 tgcccgtatt aatcggtata tagccaaaag tctttttaaa cagttcatca ccaagaaacc
     1441 acgtctgaaa tatatctcgc atagtcgtcc acatagttcc gttgcgtttc agccattcat
     1501 aatcaaaacc tgttttttcg gcacatttaa ttgcaagatt atcgtattca tcaataatat
     1561 tgtctgacaa tgaccttgca tattcaagca ctttgtcaaa ataattccaa tcagtttttc
     1621 cgttgcatat ccatgctata agcaccgtca cataacccaa atcccaattt gaattaacgc
     1681 ctttgccttt taaaacgctt atcagctcat cgtctgaaaa aacgcggctt cggatttttg
     1741 aaatattttg catacactct gcggcattaa ttttttgacc gctgttataa ttcagatatt
     1801 cccagacagt gagtgccgca caattccaat aattctgttc acttgttata acaccgtcca
     1861 tatcaaatat aacggtatcg tatttatcaa gaaattttgc gagtttcata tttttaatac
     1921 tttgttcctt tcagatttta tctgttttca gaaataggga cactaaattt tagtgtccct
     1981 atttag
//
LOCUS       DAWACS010000266         1984 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5737_length_1984_cov_2.338517, whole genome shotgun sequence.
ACCESSION   DAWACS010000266 DAWACS010000000
VERSION     DAWACS010000266.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5737_length_1984_cov_2.338517"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          647 a          389 c          442 g          506 t
ORIGIN      
        1 cttgacggta ctcagctttg gagaattgac atgggttaca atatccgtgc aggacaacac
       61 gatactcagc ttaacgtagc cgattttgac ggtgacggca gagcagaagt tatggtaaga
      121 actgctgacg gtactgttga cggtcaaggt aacgtaatcg gtgatgcatc aaagggcgaa
      181 acatacgaaa acagctgggc tgccctaaac ggcggtaaaa acttacaagg tccgctttat
      241 gtaacgtgtt tcgacggtga aacaggtaag gctcttgaca caatagatta tttcccgaat
      301 aatactgtcg gttcaaatgc tgcatcatta acattcggtg atgacttcgg taaccgttcg
      361 gaaagatata acagtacaat agcttatatt gacggtcagt caccgtctgc tgtatttgca
      421 agaggttatt acttcggtaa gggtatctca aatcctaatg gtcgtacagg tgccgctgca
      481 tacagcttta agaatggtaa gctaaagatg gaatggtcat ttgatacagc cgaatcaaag
      541 aataacggct atatcggtca aggtaaccac caaattgaag caggtgacgt tgacggcgac
      601 ggtaaggacg aaattcttta cggcgctttg acatgggata acgacggttc aattctatgg
      661 tgtacatatc aagagcacgg tgatgctatg caccttggtg acttcgaccc gactaaagaa
      721 ggtcttgaat ggttaaaagc ttatgaagat tacagtgcag acagtgaagt gtttgatttg
      781 aaaggtccac aactttctcc atacatcaca tctaatacaa tatttaaaaa ttcggctgca
      841 gctgctgctc aaggcgctcc taatgatcgt catcaatggg gtatttcact tcaaaacgct
      901 aagacaggtg aattctatca gatccacaac ggtatcaaag atacaggtcg tgctatgatt
      961 gccaatatcg gttacggcga ttcttggtac gctatgtggg gtgcaggttc atcaggttac
     1021 tgggacagca acggtaacga acttcctgat ctaaaggctg ctatgaacgg cagaatttat
     1081 tggacaggcg acttacagga tgaacttcaa gaccacaacg gtgcaggtaa agaaattact
     1141 gtaacaaagt ggaatgacaa tacaaagaca ttcgataaga ttttcgaggg taaggattcg
     1201 cactcaatca actcaacaaa gggtaatccg aacctacaag ctgacttgtt aggtgactgg
     1261 agagaagaaa ttgtttctta cgcaattaca ggtcaagaca caaagaaaga aaagacaaca
     1321 atcaagggta actttgataa ggacgttgaa gttgaattag ataagactgt atatcaatat
     1381 tctctaagac tatatgaaac accatatgca acaaattata atttctatac tcttgcacat
     1441 gatgatattt acagaaactc atcagcaaca gatacaaact gttacaacca accaccgcat
     1501 atcagctggt atatgaacga ccatattgct aattcacagt acacaactca accggatgca
     1561 aatgttaaac ttgtttcaaa ttcatacaag gcaaaggcat ttgacgaatc tgctctacct
     1621 gttgcaggtt caacaccatc aacagattca ccgttcgttg acgttgtagg tcactggggt
     1681 aagacataca tcgataagat gtataaagca ggcgttatca acggtatgga cgatacacac
     1741 ttcgctcctg acggaactgt aacaaagggt cagtttgcaa cacttatcgt aaacgctcta
     1801 aagattgata cagattcatc attggcaagc gaacactggg caatgccatt cgtaaaagcc
     1861 gctcaaaatg ctaacctaat tgcagaagat attccgttca ctgttgaaga tttcgataag
     1921 gaaatttcaa gagaagaaat ggcctcaatg gttgctaagg ctgccgcata taagaatgtt
     1981 gagg
//
LOCUS       DAWACS010000267         1982 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5744_length_1982_cov_1.807473, whole genome shotgun sequence.
ACCESSION   DAWACS010000267 DAWACS010000000
VERSION     DAWACS010000267.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5744_length_1982_cov_1.807473"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    228..237
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1326..1335
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          589 a          510 c          287 g          576 t
ORIGIN      
        1 tcataccgtg acgtataagt attatcttca tttaattctg ttccccactc cgcatacaac
       61 tcgcacgact tcgtccgctt ttttcgcgat ataacagcac gttcttccga ctgtttcgcg
      121 ccattctctg tcacctttat ccataggaac aacgccgtaa ccgatttcgg tggaaataat
      181 cgttatatcg tgatttttct ccaaaatctc gtcaataacc gcaataannn nnnnnnntcc
      241 ttgccgtcag ccatcaattt ttttataaaa atatgaaaac cataaaccgc ctttgcagtc
      301 aaaacactgt cataatcggc cattaaaatt tcatcaatac cgtaattttc tcttacaaaa
      361 tcacgctttc cgctatccgc accgcctata acaaaacgca taatacacct ccgaaaatca
      421 cgcacaaaac ctcgcatata catacaaaat atcccgcaat atcgcctgtt attccgccga
      481 atttactgta tgcaacgtgt ctgtaccaca aaaacgaaat caccgccgcg catatcgtac
      541 ccagtccgta cacgcccgta aaacaaagca tcgcaacgca caaagtaata tacaaaatca
      601 taacagtcac aatcgttttt ttgtgtgcgg cactcttaaa tgtataaaga agtccgctgt
      661 tttttgcaca tttaaaaatg ccgattgcca atccgctcag cgaacgcgat aaaacaaagc
      721 ctacactcac cgctatggta gcttttatat cccatatctc cgtcataaat ccgaaaaaca
      781 acagataata cattatcgca ccgattatcg caaatgcgcc cgtatgcgga tcttttaaaa
      841 tttcgagttt tttatctttg tcaccgtatg acgaaagtgc atcaacggtg tcaataaatc
      901 cgtccgtatg tattccgccc gaaacaagca ccggcaaagc tgtcatcaca accgcataaa
      961 cgcttgtgtt aaaaccaaat ctaccgcaca gaaatcgtac aagtaacata agtccgccga
     1021 ttactgcacc gacaagcgga aaaaagcaca ttgcgtattt catatttttt tcgttccatt
     1081 ccactctcgg cataggaatt tttgaataca ttgaaaatgc tacgcatatt gatttaatta
     1141 tcttcatttt actgtcccct tttaatttca atcggtattc cgtaaacgac ctcatacact
     1201 ttatcggcac gttttgacaa tgcactgttt attctgccta aaaggctgat atacatttta
     1261 gtgtccccgt catattcaat gccgtccgaa gatatttcat ttgtaacaat aacaatattt
     1321 tcactnnnnn nnnnnattcc gccgataatt ttttcaaaag cattatcatt attatccgaa
     1381 aacatcacgt ttgcggtcaa attcgacata cattccaaaa gaattgtatc ggcactttcc
     1441 gttatttcgg ataaatcagt ataacattca accgttctaa agcctttgcc gtcacgcatt
     1501 tttctgtgac gttcgatacg ttttaaacat tcgtcatcat acggtttcat cgttgcaacg
     1561 tatagcattt taccgcccaa tttcattgca atattttcgg caaattcaga ctttccgctt
     1621 ccgcttccgc ctgttacaag tataaacatt atttaagctc cttatagctt tcaacgttaa
     1681 tatcgtcaaa agtagacatt tcgttatata cgcaaagtgc ggtatcaagt gcggcaagca
     1741 taaccattcc gcctgtcccc tcgcccaagc acatatccgc atttatcggt gctttcttgc
     1801 cgagtaattc caaaatatca gccgtacagc tttcttttga aacgtgtgac gcaatcataa
     1861 aatcactgca tttcgggcat aacttttccg cacaaagtgc cgccaccgac gaaataaatc
     1921 cgtcaataac cattgcaaca ccgtacatag ccccgccgat aaacgccccc gtcaaagcgg
     1981 ca
//
LOCUS       DAWACS010000268         1976 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5765_length_1976_cov_1.717335, whole genome shotgun sequence.
ACCESSION   DAWACS010000268 DAWACS010000000
VERSION     DAWACS010000268.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5765_length_1976_cov_1.717335"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1634..1643
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          579 a          328 c          462 g          597 t
ORIGIN      
        1 acattctatt gacagactct ttttttgttt aaaaagtgtc acatacacaa aaagtcggtt
       61 tatagccaat agtgttttca aaatcgcttt taatcatatt tattgcataa tcgaccttat
      121 cttcttcaac gattgcccaa atacctttat cctgacatct tgcggcggct atgccgtctg
      181 tattgcgtgc agtgtttgca aggaatttat gttctgtgcc aatatcccaa aaacgttcca
      241 tggattgttc cgaattgttt atttcgcgga aaagtgtttt tatatcacat cgttttaatg
      301 cggcggaaac ggtattaatg cgtgtatttt cggtggatag gtgatacata tatttagccg
      361 ctttgctgtt tttaaacgat gattttgcgg tgctcagtat ttcgggcgtc aagtctgata
      421 ttgaattgat gtgagggtat aatcttcgta tttcggacat agcatattta atgtgctttg
      481 aatggttgct taacggttct gtgcagtgtg cggtaagaat tttgtagccg gaaagcggca
      541 acggcaggtt tttcggctca ccactgttca taagtgtaca gtagcctttt ttagaaaaaa
      601 gtacggcgag ataaggcgtt aaatcgtcgt ttaatgcaca tattgcggcg gtttcaagag
      661 gggtgagttc tatatcactt acttgcataa gtgattttat cagtgcagcc gaaaaagcgt
      721 gttttgagga tataaaattc ggtatcgtgt tattgtacag tatttctgca ccttttaaac
      781 cgctgttttc aagtgcgtgt attaactcgg caaagaattt tgttttgcct ttaaaaaggt
      841 ctgtggacgt gccgaaaggg tattgcagac atttgtctgt ggtggtgttt tcgattttta
      901 catagttgcc gccgcttttt cgtgcgaaca tttcaactcg cattgataat gtacagcata
      961 acgacggtgc tgtgggtata tctgtatgtc cgagaagtgt gcataatgcg ccgtatgagg
     1021 tgtagtgaag aaatttgcct gatgtttcgt accgcttgta aaagtcctct tttaaagtga
     1081 gaaaattcaa aaaaatcact cctttttatt agcttgccca taattttgaa ttaaattcat
     1141 tgttgattga agtgagagtg cgtagggggc gacagatggt cgccggtacg aagcatatgt
     1201 gattggtgta actctaacgt caatattaat atatttccgt aggggcggtc attggccgcc
     1261 cgttgagaaa atgcaaaagt gggcgaccaa tgattgctcg tacgaatgga taagccgtat
     1321 caaaggctcc ccttgagggg agctgtcgac aaagtcggct gaggggtggc taaaataaga
     1381 acatcacacg aatagaaaag ccaccacacc accgcaagcg gtacccctct cctcaaggag
     1441 aggcaaggaa cgacgaggca ccgtcccgta caagttggga aatccatact aaaaggctcc
     1501 tctcgagtgg agctgtcgac aaagacgatt gaggggctaa atatatattt ttttggtgaa
     1561 tgatgaccgc ctctacgaat gttttgcttg aggggagaaa ttagtgttaa aatcccaata
     1621 cagcttgaca attnnnnnnn nnngttataa tagttaaatc gtaagtgaaa ataagagagg
     1681 aatgacataa aatggctgat gaaaaggaat ttgttttaac acgagaaggt aaattggaac
     1741 ttgaaaacga acttgatact ttaaaaaatg ttacaagaaa agaagtatct gaaaaaatta
     1801 aagaggctcg ttcatttggt gacctttcgg aaaaagccga atatgatgaa gcaaaaaatg
     1861 aacaggcaga agtagaggca agaattaacg aaatcgaata tatgcttaaa tatgcaagag
     1921 ttattgacga tgaagaaatc aagtcagacg ttgtaactcc gggttcaaag gttgtt
//
LOCUS       DAWACS010000269         1956 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5835_length_1956_cov_1.624408, whole genome shotgun sequence.
ACCESSION   DAWACS010000269 DAWACS010000000
VERSION     DAWACS010000269.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5835_length_1956_cov_1.624408"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1143..1152
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          615 a          325 c          524 g          482 t
ORIGIN      
        1 taaaccgaaa atcgaagtaa acagatttgg aaaaaaagcc gttacatata tgggtatgaa
       61 tcagacaaca aaaacttgga gcagacttga aacatggggc ggtaagcttg ttgaaaacat
      121 agtacaggcg tttgcgaggg attgcttggc tgaaagcata attcggcttg aggacagagg
      181 ttttaagatt aatttccacg ttcacgatga ggttatagtt gacgttccga aaggcgtgtc
      241 gagtgcagag gagttggcgg cgataatgtg tgagccgatt gaatgggcga acggacttcc
      301 gcttaatgcg gacggatacg aatgtaattt ttatatgaaa gattaggggg tgttataaat
      361 tggatttagt aattgctacg ggacaaagca gaaaatcaaa actatggaaa aatacaaaaa
      421 tgtcgtgggg agattttgtc gaaaggctga aaacgacaac aaggacgagc gaaacgcaag
      481 gtgaatttgc aaatatgccg aagtcacaac aggatgatat aaaggacgtc ggcggttttg
      541 tgggcggtaa ggtgaaaaac ggcaagcgac agtcgggaag aatcgaaaac agaattttgc
      601 ttacgcttga cgcagacttt gccgacagtg atttttgcga taatatttca atgttttacg
      661 actttacata ttgcatttat tcaacgcata aacacagtgc cgaaaaaccg agatttcgtt
      721 tggtgatact tctgtcaaga ccttgtacgc ctgatgaata cgaagcggtt gcaagaatgg
      781 tggcgtatga tattggtata gatatgtttg acgacacaac gtatcagccg cactgtttaa
      841 tgtattggcc gagtacgagc attgacggcg agtatgtgtt tgaacacgag gaaaataaac
      901 cgcttgacgt tgacagggtg cttgcaaaat atgaagattg gcacgacgta tcgagttggt
      961 acgcttcgtc aagaacaaca aaggcgttgg acagacaggt aagaaaacaa gaggatccaa
     1021 cgcttaaaaa aggtgttatc ggtgcgtttt gcagaacgta cgatatacat tcgtgcatag
     1081 aaaaatatct tcccgacgtg tatgaaaaat gtgccgtagg cgacagatac acatacaggg
     1141 acnnnnnnnn nncggctcga gttcaagcgg acttgtagtg tatgagaacg gcaaatttgc
     1201 gtattcaaat cacgcaacag accctgcaag cggtaagttg tgcaacagtt ttgaccttgt
     1261 tcgtattcat aaattcagcg atacggacgc agacgcaaag gacggtacac ctgtatcgaa
     1321 actgccgtca tattcggcaa tgtgcaagct tatagacggt gacagtgatg tttcaatgct
     1381 tatgtttaag gaacgtcaaa agaaagcggc ggaagatttc ggcggtatcg aaagcgaaga
     1441 aacggacgat atgcagtggg cgttaaagtt ggagaaaaac gaaaatacag gcgcttacga
     1501 aaagacgctt aacaatatta ttcttataat tgagaacgat tcgcatttaa aaggcaaaat
     1561 caaaatgaac gattttacag gctatgcaga gattgacggc attatgcctt gggacaagga
     1621 cgcaccggaa aaacgcgttt ggcaggattc cgatacggac ggattgcagt ggtatcttga
     1681 atatgtgtac ggcattaaaa tgggtaacga taaggttttt cgtgcgttgt cagtgtttta
     1741 cagacgcgtt gcgtatgatc cgattgttga gtatttggac ggtcttgcgt gggataatac
     1801 ggaacgactt gacacattgt ttgtcgatta tctcggtgcg gcggataacg aatatacaag
     1861 agaagtgacg cgtaaaatgt tcgtcggagc ggtcgcaaga gcgtatgagc cgggaagtaa
     1921 attcgataat atgcttattc tgccgggcag gcaggg
//
LOCUS       DAWACS010000270         1954 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5838_length_1954_cov_1.968404, whole genome shotgun sequence.
ACCESSION   DAWACS010000270 DAWACS010000000
VERSION     DAWACS010000270.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5838_length_1954_cov_1.968404"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    991..1000
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1281..1290
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          489 a          456 c          310 g          679 t
ORIGIN      
        1 catgctgctt tttgattttt acttctccgc cgccgttatt tattacactt ctgcggtatt
       61 gaagttcagt caatttatca actgttccca tgttttaacc tccattgttt gtagattaga
      121 gataaatttt aaaaaaatat acctaaagta tattatacct taaccgcaat tttttttcaa
      181 tcatttttat ttcttttttc acaagttagc tttcaaaaaa tatcgacaaa tatcccctta
      241 aatttgtata tgatacacaa aagaggctgt tgacaaaatg tcaacagcct ctttttaaga
      301 attcatcaaa taatttactt cgtgacttgt cagatgacgc catcttccaa gcggaagatt
      361 accgagttcc acgttgccta tcttaattct ctgcaagccg acaaccgtac agccgattgc
      421 cgcaaacatt ttacgaacct gtctgttttt accctcgtga attgttattt taatatatgt
      481 gtgtccgtct accgcgtcaa gtatttcagc ctccgccggt gatgttttga aatcatctat
      541 ataaacaccg tttctcagct tattgatacc gcttatcgtt ataccgccct ttatcgttgc
      601 gatataagtc ttatcggtat ggaacttagg gtgcgtgact ttgtatgtga agtcaccgtc
      661 atttgtaagc aaaagcaatc cctcggtttc atagtcaagt cgtccgacag gaaaaattct
      721 tacgtcaccc aaatcttctt ttataaggtc aacaacagtc ggtcggtcaa attggtcttt
      781 tgcggttgaa acatatccga caggcttatt aagcattata taatacttct tgtttttaag
      841 ctttatcggc ttatcgtcaa cggatacatt gtctgcaccg atttcgattt ttacgccttg
      901 ttccgttatt ttcttgccgt ttaccttaac tctgccctct tgtatcatag tttctgcggc
      961 acgccttgac gcaaccccgc tcattgctat nnnnnnnnnn atactgtttt tcaatcttat
     1021 attgcttact ccgtaaatat cgttttccga aataatatca acttcaccga tgctcactcc
     1081 gtcacacaca atttcaagcc accccacttt ttcgccttga tttataggtg cttgtaagtc
     1141 atttgatata tgcgatataa cttctacctg cgttttctga tgttctttaa acggtattgt
     1201 aaagtcactc gccgcaacca ttgaataatc ttttccgtca atattcgtaa cctttacagt
     1261 atcgccctgc tctataagtt nnnnnnnnnn taatcaagca ttttcgcgtg gtcattccaa
     1321 tcgttcggat cattcaaagt caccgctata aactccattc cgtccctctg tgcagacgat
     1381 acaagacatc tgcccgttga ctttgtaaag cctgttttta tgccgtttgc tccctcatac
     1441 agacttagca ttttattgtg gtttgaaaaa taaagtattt catctgaatt taatgcctgt
     1501 acttgcgcag tctgcgttgc gactatttcc cgaaactgcg gcagcgtcat tgcatatctt
     1561 gcaataagtg caagattgta aaccgttgtg taatgttccg gattgtcaag tccgctcgga
     1621 ttcataaaat gcgtattgcc gagtccgagt tcttccgctt tttcattcat cagctccgca
     1681 aatgattcct cacttcccgc cacatgttcg gctatcgcta ccgccgcgtc atttccggaa
     1741 ttaagcatta aaccgtaaag caaatctttc atataatatt gatttccctc agatatgtat
     1801 gccgccgaac cctcttgatt tgccgccgtc gcactcacac caacaatttc gtccatatta
     1861 caacgttcaa tagcaacaat ggctgtcatt attttagttg tgcttgccat tgcgtgtttt
     1921 tggttcatat tttttgaaaa tacaacatca cccg
//
LOCUS       DAWACS010000271         1949 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5857_length_1949_cov_2.497360, whole genome shotgun sequence.
ACCESSION   DAWACS010000271 DAWACS010000000
VERSION     DAWACS010000271.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5857_length_1949_cov_2.497360"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          646 a          331 c          468 g          504 t
ORIGIN      
        1 gatgtatact tcgcgaaagc ggaatattca accgataatg cagtcggaac agcatatctt
       61 gcacatctgg cacataagga ggcataggct tggaacctaa aattgctacc gtttcacaga
      121 taaacggtta tgtaaagaaa atacttgacc ataatataat acttaataat gtttggataa
      181 agggcgaaat atcgaatttt aaacatcatt attccggaca tctttatata acgcttaaag
      241 acgagggcgg agtgcttaaa gcggtaatgt tcagaggcag tgcgcagtcg cttaactttg
      301 aaccgagcga cggtatgaaa gtccttgcac gaggaagagt aagtgtgtat gaagcaggcg
      361 gttcgtatca gctttatata gaggaaatga tacccgacgg cgtgggtgaa ctttatattg
      421 catacgaaca attaaaaaaa cagcttgaag aagaaggtct tttccgtccc gaatacaaaa
      481 agcctattcc gaaattccct aaacgtgtcg gagttgttac cgcttcgaca ggtgcggcgg
      541 taagggatat tataaatgtt ataacaagac gttatcctat ggctgaaatt gtgatatatc
      601 ctgcacaggt acagggtgtc ggtgcggcgg aaagcgttgt taaagcgatt gaatacttta
      661 acgcaacaaa tgaggttgat acgcttattg tcggacgcgg aggcggcagt atagaggact
      721 tgtgggcatt taatgaggaa atcaccgcaa gggcgatatt taattcaaat ataccgatta
      781 tatcggctgt cggacacgaa acagatttta cgattgccga tttcgttgcg gatttgagag
      841 cgcctactcc gtcagcggcg gcagaaatag cagtaccgtc aatgattgaa ctcagaaaca
      901 gaatagatac cgataaaaac cgtatttctc aaaatatagt caagcgtatc gaaagctgta
      961 aattgctttt aaaacgattt aaaatgcgta ctcctaagga taggattgac gattattatt
     1021 tgaagataga ttcgcttgtt aaatctatgg ataactgcat taaaatgaaa actatgtcgc
     1081 taagacgtca gcttgcggaa caggcggcaa aacttgacgc gttaagtcct ctgcaaactc
     1141 tttcaagagg atattcgatt ccgacaaaag aggacggaac tgttataaga agtgcgaacg
     1201 atatggaaaa gggtatggaa tttacactta gaatgaaaga cggacagaca aattgtgtag
     1261 taaagggaga atagatatgg caaagacatt tgaacagtca atagctgaac ttgaagaaat
     1321 tgttgaacag cttgaaaacg gtgacgtgac tcttgacgag tccctcggac tttttgaaaa
     1381 gggaataaag ttatctaaaa gctgtcagaa aatgcttgac acggcagaaa agaaagtttc
     1441 tatactgatg acaaatgatg acggagaaat agtaaaagag gactttacag gtgcggacaa
     1501 tgaataatat aaaggaattg ctgaaagagc agattgaatt tacagacggt tatcttgata
     1561 agtaccttgc cgaaaaagac aatccgcaga atattatata caaggcaatg cgatacagtg
     1621 tttttgcggg cggtaaaaga cttcgtccga tacttatgat gaatacttgt aaaatgtgcg
     1681 gcggtgatgt aaatgaggtt attccgtttg cgtgtgcgct tgaaatgata cacacatatt
     1741 cgcttattca cgacgatttg cccgcaatgg ataatgacga tttacgacgc ggtatgccga
     1801 ccagtcatat taaattcggt gaggcaaccg ctattttggc aggtgacgca cttttgaata
     1861 aggcatttga agttgtgtcg cagtacaacg gcaacaatcc tcaaagggcg gttaaagcaa
     1921 tgaatatgct tgccgtatcg tcaggtaca
//
LOCUS       DAWACS010000272         1948 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5862_length_1948_cov_1.980454, whole genome shotgun sequence.
ACCESSION   DAWACS010000272 DAWACS010000000
VERSION     DAWACS010000272.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5862_length_1948_cov_1.980454"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          600 a          324 c          376 g          648 t
ORIGIN      
        1 tataatcggc ggtatcgttg cacttgtgat tgttacaggc ggttactttg ttgcggaaat
       61 tccgcttatg ggatacgaaa ccgcacttgt atcgcttatt tcatcaccga tacagtggtg
      121 tatgagtatg gttgcatcgg cgattattgt accgatattg attaaaaaca gaaaaagaat
      181 aggattttaa ttaaaaacac acagtctgtt ttcataggac cgtgtgtttt tttagttatg
      241 tactttattc actctttgga gcggactgtg ttttttttga ggggttctta gctgtataag
      301 ctccaataat tttaaaatac tgttttttaa gccatatttt acgattggtt tttctatata
      361 gttggttatg attattcccg ccaatattga aacagtccaa caaattaagg ttaatccgtc
      421 cattgcggct ctgtcgttca ttacgggatt ttgtgtgctg taaggtatgt tgtgattttt
      481 taggaatata aatacattct ggtgccatag gtacaccgta tatgatattg atgaaaggaa
      541 aacgaaaaat ttatttcccc atattttctt ttcccaaaaa ccgtatgaga aacaagcggt
      601 gaaaaggaaa agtgctatta atacagagaa tattcctctg tacaaaaagc gaaaatacac
      661 atcgcccgca actccgttcg ggaatgaaat ctttctaagc cagcacatat atccgaaagc
      721 agagaaaata ctcaaagcgg atataattgt catgcacaat ttgagtttat ctatacattt
      781 tactttgttt cttacatata ccacgaaata tgcggaaatc attccgaaag caaatacgtc
      841 aaaataaata ggtgtgattg ctgacattat aggctgtgat atgtttgcat ttgccataag
      901 tataaatctc agcaatacag ccactaaaga cataacgact gttgtaagaa caggtttctt
      961 gcggaacgga atacatataa gcggaaacag aacataaaac tgaacttcaa tactcattgt
     1021 ccaagcggta gaaatggttg ttccgatagt atgcgaatca aagttatgaa tgaaaagcaa
     1081 atgcattata acattgtgag caacattcgt aaaacttttg ggatcataca ttccgcctat
     1141 atatgaaaaa ctcggaatta gaaaagtcag aataagcatt atgacatatg aaggataaat
     1201 tcttcttgcg cgctttataa agaaattctt ccaacctctg aactcacatt ctccaaacat
     1261 acttcgtgcg ataggataga aaagacaaaa tccgctcaac acgaagaaag aatctatggc
     1321 tacatagccg taacgttgaa atatttcaaa attaaacaaa tacttgtcgg ggcttatttt
     1381 taaattataa aaaatccaac tttgctggaa tgtgtgaaat attactataa aaataagtga
     1441 tattgctctc attccgtcca aagcgtaaag atagccagga atatcgcttt gacgtgaatt
     1501 attcatttgt ttccctcctg ctgtacgtaa tgccgattat aacggtgacc aaaattattg
     1561 cggcggttat actaagacct ataatcatta ttttaggcat atgaccgcaa ctcattattg
     1621 cccattttat tctttcgata cctttttcag cgggtaaatc ggctgcgtat gcaaatgtgg
     1681 aaggcatagt gcataacatc acccaaatag ctaatataaa ttttttcata tgttttcctc
     1741 tctgtaagtc tataatataa gactgtttat tgaattataa ttgctgtacc cgattgtgca
     1801 ggggcagggg ttatgttttg ggtattttgt gcctgtacag gtttgtctgt tgccgattgt
     1861 gtattgctgt tttgtgcgtt tgcggtgtcg gctgttggat ttgttgtact tgttgggttt
     1921 gttgtatttg ttggatttgt tgtgcttg
//
LOCUS       DAWACS010000273         1945 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5877_length_1945_cov_2.571429, whole genome shotgun sequence.
ACCESSION   DAWACS010000273 DAWACS010000000
VERSION     DAWACS010000273.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5877_length_1945_cov_2.571429"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          484 a          450 c          296 g          715 t
ORIGIN      
        1 gccgttcttg cccacgacgg catattgtca tcattgaaat cgtaaatcat tgtagtttga
       61 actacattca ccaactgttg gttgatgttt tttaaatcgg caatttccgc cgactgtttt
      121 tcaattaatt cttttaattc attgtactgt gacattgtta gatcctcact ttcttcgact
      181 atactccaat ccggtgtact gaattttgtc cccggtaaat tactgttata ataacctttt
      241 cggcaaacac cgccgccatt tgcaataatg gtactacctc cgtttgtgtt gccctcgatt
      301 gttgtaaaat aatcgccgtt tacgcttgta acaattcccg tatgtgtaaa tgttccgttg
      361 tgtttgaata taacaatatc gcctactttt ggatttgcat ttaatgtgaa caaatttgcc
      421 attgtaggac aatacacata cggataatgt tttaataatt tttgtgcatt attttttcca
      481 aacgcattga caaaacacca cgttacaaaa cacgcgcacc acggttgtcc ttggtattcg
      541 ggtttaatat ctcgccaata ttttgtgtag ttggcagtac cggcatttgc tgtcttgctg
      601 tctaactgtg aattacttga cttttccaaa tagccaacct ctgcattggc tatttgtatc
      661 aatttatcta tcgccgacat tatcaccact cctttacggt catattcttc cattttttat
      721 atgcgtcaaa atacatctca tttttatcac cgttatatgt aatttcgtaa tacattccgt
      781 ccgatacagt tgttgacgcc aacgctttaa aattctgcaa tgtcttacag ctccacacga
      841 tatacacatc atctgtggtg attttcttac cgtctgtcac atcaacattg ttgttgaaat
      901 agtttgcgat taatgttttt actgcgttta taaaaatttt atccgtcata ttttattaca
      961 cctctttcaa ttcaatatct tccattacta ctcgtgcctc taaaattgcc aaatagtcag
     1021 ccattgcgtt tagttgtatg ttatatgtac tgcgtggaca cgttggggaa aattttagtt
     1081 ttcccctgtc ccattcctcc aacattttct ttaatccttt gaatctattg gctaattgat
     1141 aatattctgc cttgaaacgt tccttgtagt ccgcactgtt catcagtgca acggtatctt
     1201 gtaacgtcat attttattcc cccttttcgt tattcatttc cggcaatccc gttgcaactg
     1261 atgtcaataa cgacaataca cctgccaatg ccgccgctga cgcaaccatt acccagttga
     1321 cgtcacccaa tgcaacagca gtgccaatag tggcaactgc tgtttgtgca actgttttta
     1381 ttgcacgaat acctgcacat ttaaaccatt ctttcatttt tatgtacctc ctaaaatact
     1441 aaaatcctaa cattttaaca aaataaccta tcaaaccgcc cactattgcc gtaataatgg
     1501 cggtaacgac tgtttcgtat cgctttgtag gacgtttttc tatttcgtct acccgtgatg
     1561 taatatcgtt tacgtcctca cgcattgcct tagtttccgt ggctatgatg tggacgcttt
     1621 cggtcagttt ttcgataccg tcaattctgt ggtgtgccga cttggtggac tgctctacgg
     1681 cagtcagacg ctcccacact tctttttcgt tttctgcctc catatcagcc ctccataaat
     1741 tttctctttt catctgacgt gatatatccc gctttgacga atatatctaa atgcttttct
     1801 ttgtaaataa ccttttttaa cattttttta tcagtgcttt actcactttc gatacctgct
     1861 ttcagttcca caatctacat caacatcatc tgcattaatt ctttgtattc atcatcagta
     1921 atacgttctg caagaagaaa cacgt
//
LOCUS       DAWACS010000274         1942 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5888_length_1942_cov_2.100159, whole genome shotgun sequence.
ACCESSION   DAWACS010000274 DAWACS010000000
VERSION     DAWACS010000274.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5888_length_1942_cov_2.100159"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          629 a          370 c          479 g          464 t
ORIGIN      
        1 gatgatgccg gcaataatct ttacggcgta ctcactaaga cgactaaaaa aggcgaatgt
       61 gagtggaatt ttgagttgcc tgtatcgaat cagttcagct acaaaatggt tgtaaagaaa
      121 caatcgcacc ttacatatcc cgaaatcgaa atagataaga cgcaagtgga tgataatgca
      181 gatttgacgc ttgccgatac tattgaaatg attgttggtg atataaacgg cgatgaaatc
      241 atcaaattgc ctgatagggc ggagttgatg agatttttta accgtcagaa accgtggata
      301 ttggataaag cacggtttga ggcggcagac ttaaatggtg acggagcggt taatatgttt
      361 gatctgacac ttttgaaaca aaatatggag aaaacatatc cgtctaatat aactaatgca
      421 aataatggag gtggttccgc ttgataaaga gaatagtagc agttatgtca gctcttgcgt
      481 tggcagtggg attgcttcct acggtgttag ccgatgaagt gacatcgaca aagccggaga
      541 taactgttgt tgcaaaacgt ataacaggtg aagataatta tatggaaatc agccttgaag
      601 taaacggcga ttacgaagat tattcatcgg ttggagcggt tttgcaatat gatccgagcc
      661 ttatagttcc gactacatgg gacgatgagg caacagcggt ggatatgagt gagtcaacca
      721 catgggctac aaggcgagcg ttgccgacgc tgggtaaaga cacatggaca actcataccg
      781 cgctgacata tattgaaagc aaaacagata cggcaacaaa tcagacgacg aaaacgggtt
      841 atttgtattt gggggcggaa tatccgggtg tagtaccgaa taatgcgaca gacccaagtg
      901 caacggcgga tccggatgcg acaccaacac cgacaaagac gccgaccgta acaccgacgc
      961 ctgacccaaa ggctacgcct gatcccgacc aacatcaaaa tccggttgtt gtagcgagat
     1021 tcacatatgc cgatgaaaat gcgaaaaaga agatagaaga tcaatggact tccgcatggg
     1081 atagtgattg gacgcagaat aaagtgctga caatcgcgtc tgatgatgta tcttccgtat
     1141 caccggcaca gttcggcttt acgatttata aagcggactt ttcaatgaaa ggttatcagt
     1201 atgattatac agatagcgtt cttgaaatga gtgagcaagc ggcgaataag gatattgaaa
     1261 ttgtactcgg acaaggaaag agtgcgaaag cgggcggact tagtctaaac gatatttacg
     1321 taacaatgtt ttacgattgg gacgattcac ttatcggcac actgacatgt ggtgtcggag
     1381 aggacgcgac cgaatcggta aatgattatg taaaagaaaa gttcattcat cctgacttgc
     1441 agacgaatac gaattattca agcaaggaaa gaacggataa ttatagaggg gagtatcctc
     1501 atgccggtcc aaattcaact gacccgacaa ctccgggcgg aacgggaacg gttacgaatg
     1561 gctcagatta tccactgacg aataaacttg aatattgctt tgcaggtaaa acgattcatg
     1621 cagacgcacc gtatgcaggt ggctggacaa aagtatctgc ctcaaatatg tcagatacat
     1681 ggacggcact taataatgca accacattcg acttaatgcc ggaacttggt gcagacggta
     1741 atccggttga ttcaagtcaa acaaaaccgg atatggaatc attcgatttc tccgatgtta
     1801 ccacagatga tatagacggc ggtacactgg tagtaaaagc cgtatataca ccgggaacaa
     1861 atttagactt taatggtgac cgatactatt cagcgatagg acctcttcaa tatgctcttg
     1921 taacgtcaaa tgcaacatca ag
//
LOCUS       DAWACS010000275         1939 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5901_length_1939_cov_1.978238, whole genome shotgun sequence.
ACCESSION   DAWACS010000275 DAWACS010000000
VERSION     DAWACS010000275.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5901_length_1939_cov_1.978238"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1531..1540
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          472 a          487 c          302 g          668 t
ORIGIN      
        1 aatgccgccg catttaccgc gccaagattg ctttgtacaa cacaagcaca agccggtgtg
       61 aagtaatcag gtgtaactgt tgcaagcatt ataaggtcaa tatcctcagg tgtaagtcct
      121 gcattttcaa tagcacgctt agcggcgatt gttgccatat ccgttgtata ttcgttgtcg
      181 gccgaaatat gtctttcctt tatgcctgta cgtcttgtta tccattcgtc gtttgtatca
      241 acaatacttt ccatatacgc attgtcatat accttttccg gaacatatcc gcctacacct
      301 gttatctttg cattaatcat tactcttttc ctccatacag tttatattat taactatttc
      361 ttctattaag tttgtttcaa caaatttctt tgcctgtctg attgcagaaa ataccgcttt
      421 accgtctgac gagccgtgtc cttttataac gggtcttttt gtacccaaaa gcggtgcgcc
      481 gccgtattca cggtaatcca tagactgttt aaactcattc aagccgccca ttacaaatat
      541 tgcaccgagc tttgtaaaca agtttttcat aaatatattt ttaactttcg tacttacgac
      601 gtgtcccata ccctcaacgg ttttaagtat tacatttccg acaaaaccgt cacaggtaat
      661 tacgtcagcc gtaccttcca taacatctct gccctcgata ttgccgataa agtttatagg
      721 tgcttttttc aaaagcggga atgtttcctt cgtaagttca tcgcccttgc cttcttcttc
      781 accgtttgac ataagtccga cagtcggact ttcaattccg agtacattct tcatatatat
      841 actgcccata ataccgaact gaacaagatt ttcaggtttg cagtttgtat ttgcaccggc
      901 gtctatgagc attttcggtc cttttgcact cggcagaagt gttgcaagtg caggtcttaa
      961 aatccccttt attcttccga caataagcaa tcccgacgct aagagcgcac ctgtattacc
     1021 cattgaaagc attgcatcac cctcgccttt tttcagcata tttgccgcaa cgaccaccga
     1081 tgcatttttc ttacttctta ccgcctttgc cggttcttcg tggtttgtta tcacttcatc
     1141 agcattcgct atactgattt tatcatttgg gtagtcatat gccgaaagtt ccttttcgac
     1201 aacctctttt ttgccgacaa gaactatttc cacatcaagt tcctttaccg ctcttacagc
     1261 cgcctcgaca ggcgccatag gagcattgtc accacccatt gcatctacta taatcttcat
     1321 ctttcctccg actatttcac atcagtcata acaaggctga atttccctct gaatacttcg
     1381 ttcatatttg cctttatgac aacacgaacg atatatcctc cgtcttttac tctcataacg
     1441 tccgacttgg caatcaatct ttcacctgcg tgtgcttcgt gcatatattt cacatttgcg
     1501 atttttacta acgccgcctt cgcgtcaata nnnnnnnnnn tatatacact gtccttttat
     1561 tatgtcagta ccgtcaaagg tcatcgtatc atcggtttta agcaccgata ttccgtcatg
     1621 atacaagtta aggtcaagca cttcgccaac cgctgtatcc gctaccaaac cttcttccgc
     1681 caccgacttt attctttcac gatattctgc tataccaagt tccgctctgt caaaacgaat
     1741 agtggacacg ctcacgttac aagcctgtgc aagttcttcg tctttcaaaa acggattttg
     1801 ttttattttt ctgagcaaat actcatgacg tttctttttc aatattgttc ttgtcacttc
     1861 aagcgttcct ttctatgttt ttcgacattt tattaattat tgttagtagt aggtactaaa
     1921 ttaataacct caatgatta
//
LOCUS       DAWACS010000276         1937 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5908_length_1937_cov_1.311371, whole genome shotgun sequence.
ACCESSION   DAWACS010000276 DAWACS010000000
VERSION     DAWACS010000276.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5908_length_1937_cov_1.311371"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    643..652
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1349..1358
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          498 a          399 c          365 g          655 t
ORIGIN      
        1 gtagagtata aaaaatattc ttcatattat tatatatttt ataacacgaa tagtcaagca
       61 taaaaaaggg taagaataca gctatttttg gcataaaatt tgtacaaaaa aacacagact
      121 gtatttgttt tacagtctgt gtatatttta catagtttgt ctgccctcaa gagcctttat
      181 aagagtaatc ttatcggcat attcgaggtc gcttcctatc ggaataccgt gtgcgatacg
      241 ggtaactttt acgtcaaacg gagcaagaag tttagagata tagactgctg ttgctgtgcc
      301 gtttacggtt gggttggtag ccataatgac ttccttaacc gtactgtcgc tgagacggag
      361 taaaagctcg ttaatcttta tgtcatcggg ggttataccg tccatagggg acaatgaccc
      421 gtgaagtaca tggtaaagac cgttaaattc gtttgtcgcc tcgattgccg aaacatcttc
      481 gggactttcg acaacacata taacggaatt atcgcgtttc ggtgatgaac atattttaca
      541 cggtgatgta tcggtcagat tttgacatac agggcaaagc gtaatttttt ctttcgcctc
      601 tgtgattatg ttagacattg aaactgcgtc ctcttttgac atnnnnnnnn nnatgttcaa
      661 gaatgtgaaa agcaattctt tctgcacttt ttctgccgat accgggaagt tgttcaaatt
      721 tttcgattaa attttcaatg actgttgcgt gcatattaga ataagcccgg aatattaatt
      781 ccgcctgtaa cggcactcat accttcagcc atcatatcgt cagccttttt gactgcatcg
      841 ttgactgcga ccataacaag gtcctcaagt gtttcaatat cttcaggatc aacagcgtca
      901 ggactgattt taagtgaaag aatttctttc ttgccgttag cggtagctat aactgcaccg
      961 ccgccagctg taccttcaac aaccttatct tcaagttctt cctgaacctt ttccatttca
     1021 gcctgcattt tttgagcttg cttgataacg ttgctcatat tcttattagc gttcattcct
     1081 gaacctgcaa aacctttgta ttttcccatt gtaatttacc tctttcttag tttttaaaat
     1141 taattattat catcctcagg ggataattct ttgccttcca aaaattcttc gggagcattg
     1201 tcatcatctt cgctattgtt atcgtcaaga gatgactgtg agaagttatc cttagaggaa
     1261 tcatattccg taaattcgct ttcgtctaca ttttcgacga tttcaccgaa attcgctaca
     1321 agattgtcaa ggggatcgtc ggatacttnn nnnnnnnntt catttacttc accgttgcca
     1381 ccgggcaagt tccaaaaatc aacgagttct tcttgtatgt cgttttcgta tgcaaccttt
     1441 accgcaaagt ctgtaccaga cgcacgttta aaaataacct gcattttatc cttgtacgct
     1501 gccgctatat tgtaagcacc tttctctttg gggttgaaca aaagtataat accgtctgaa
     1561 tcaatggtta tatttctgtt catcaaagcg gcttttaagt aacctgccga gttgcacatc
     1621 gttcgcgata tgttgtccca attctttgca accttgacaa taggattgtc ggaggagagt
     1681 tcttctttcg gaattgggtt atataatctg accgaagttt tcttcttctc ggttacttct
     1741 ttttttttaa ccgtttcggt cggctgcacc gtcacaccgt tttttacctt ttcctcaaga
     1801 tttgaaattc tttcggatag ggaagaagta tcggtaacgg caccgttttt aactttgctt
     1861 tccaatgacg caagcctgtc cataaccgct gacggtgaat cgtcatattc gggtctggca
     1921 agtttgataa gtgccaa
//
LOCUS       DAWACS010000277         1927 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5949_length_1927_cov_2.719017, whole genome shotgun sequence.
ACCESSION   DAWACS010000277 DAWACS010000000
VERSION     DAWACS010000277.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5949_length_1927_cov_2.719017"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          555 a          424 c          350 g          598 t
ORIGIN      
        1 ctcttgatac ataaattccc acaccgagtt ttctgtggtc aacttgaaag ctctttattt
       61 tattcactgt aattttcctc cgtttatgct gtttaaaagt tcaagtataa tcttgattga
      121 atttgccgcc gccatttttg caaattcggg ataatccata tgtgaatcgt cgtttgcgcc
      181 gtccgatatt gcacgaagta cgccgaaagg cacaccgttc atataacaaa cgagtccgat
      241 acttgcaccc tccatttcag cggcaatagc accgaaatta tcttttattc tatctctttg
      301 ttcctgtttg ctgatgaatt ggtcgcccga agcaataaca cctctttttg atgttattgt
      361 gcctaccttg cttactgcct gctccattaa atcggcaagc tttttgtcac aaggtatatt
      421 gacaagatta ataccggaaa tcaagccgac agggtcacct atcggtgatg tgtccatatc
      481 gtgttcaaca acgctgtccg caacggctat gtcgccgatt ttaaattcgt ccgaaagtcc
      541 tccggcaaca cctacattta tcataatgtc aggcgaatac ttaagtatca tagtctgtgc
      601 acaaaccgcc gcatttacct tgccgactcc cgcaacagca acaaccgttt ccacgccgtt
      661 aatattgcct ttataaaaat caatggtgct gattgtttcc accactgcat tgtccataag
      721 tccttttaat gcctgaacct caaggtccat tgcacctatt attccaacaa gcatatatgt
      781 tcctcctaac tttctatcta ttatataact aataaaattg cctgtcaatt tatgttctgt
      841 acaaataatt actctttttt gctttatcct ttgacattcg gcaaaaaaag ttgtataatg
      901 atatgacgtt aggagagatt atatggattt tgtagctatt gactttgaaa ccgccacatc
      961 aaattataca agcgtatgca gtcttggcat atgtgtggtg gaaaacaaca agataactga
     1021 cagaaaagaa attcttatta aacccgaacc gtttgagttt aatgattata atataaaaat
     1081 acacggaatt acacccgata tggttgtgaa caaacctaca cttgcggatt attgggataa
     1141 gttaaagcca taccttgacc acaaaacagt tattgcacat aatgcaggtt ttgatgtcgg
     1201 tgcactttgt gcgactttgg agcattttaa tataccgttc ccgacttttg attatttgtg
     1261 tacggtgaaa ttgtcgcaaa aagcatatcc cgaacttccg agtcataaat taaataatct
     1321 ttgtgacgca ctcggtattc attttcatca tcaccgtgct tatgatgacg catatgcctg
     1381 tgccgcggtt atggttagaa tacttgaaga ttacagtctt ttgaaccttg atgaggttga
     1441 ggaatgtttt gaaatggaaa tcggtcacct tactccggat actcatttga agtgtaaaaa
     1501 gaataagaag aaacaaaaga aaaattcaaa gaaagccgtc aatgaatgac ggcttttttg
     1561 atgttaaatt gttgttagcc acccctcagt cgtttcacga cagctcccct caaggggcgc
     1621 cttaaaagcc tctccttaag gataggtggc acgaagtgac ggaatggcgg caaaaaggac
     1681 gtcgtggtgc cgtcccttat gaagtgaatg cgattttcgg gcgaccgatg atcaccccta
     1741 caacaccaca caaaaaaagg gcgaccgatg atcgccccta cgaacaatgt ttgccccgtt
     1801 ttttggaact caatgcactg tcccatacga agtgaatgct gttataatct accatacaag
     1861 caactttctc tgctccctta cttcaattct ttaattttaa attcaccgtt ttcgtatatc
     1921 atatatg
//
LOCUS       DAWACS010000278         1923 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5970_length_1923_cov_1.756424, whole genome shotgun sequence.
ACCESSION   DAWACS010000278 DAWACS010000000
VERSION     DAWACS010000278.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5970_length_1923_cov_1.756424"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1631..1640
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          688 a          318 c          401 g          506 t
ORIGIN      
        1 cttttgttat tttcccaaaa atctttgatt ttctttaaaa aaactattga caaatgtagt
       61 ttttttatat ataatctgat aagttgagtg tgtcataaat aatacacttg acaaagtaca
      121 atttttcaga tacggagtgt gacaaaacca tgattggtga tgccgaaagg tcaaagttgc
      181 aggttggtta taaacaagct gtaagagctt taaatgaaaa caaagcctct aaagtctttg
      241 ttgccgaaga ctgtgacgac catattaaag acggagtaag caaaattgct atgcaaagca
      301 atactcctgt gttcttcatt tctacaatga aggaattggg gaatatgtgc aaaattgaag
      361 tcggtgcaag ctgtgccgtc gtattaaagt agtttattac atatcttaat gatatgcgat
      421 aatatataaa attttatgaa aggaagtgta acacgatatg cctacattta atcaattggt
      481 tagaacaggt agaaagacat caaagaagaa atctactgct ccagctcttc aaaagagtct
      541 aaactctcta aagaagaaga ctactgacaa gagttcacct caaaaaagag gcgtatgtac
      601 agctgtaaga actgctacac ctaagaagcc taactcagct cttagaaaga ttgccagagt
      661 taagcttaca aacggtatcg aagtaacagc ttacatcccg ggtataggtc acaacctaca
      721 agaacatagc gttgtgctta tcagaggtgg aagagtacgt gaccttcctg gtacaagata
      781 ccacattatc agaggtactc ttgatgctca gggtgtaaac ggacgtcttc agtcaagaag
      841 taagtatggt gccaagaagc caaaggaaaa gaaataatgc cgcgtaatta actaatacgc
      901 gcatatcaca agtgcatatc gagttatatt attatatatt cgaatagcac cacggggttt
      961 tgaaagaata aactccgagt acctgtgatg tcatttgaaa aacagaaatt taaaatgttt
     1021 ttcacggtaa agcttacagt cggagaatat cgcattcaag acgcaggtag aatttgataa
     1081 atatcaaggc acaaaacgca ggaatacttt gtgtattccg agtatttgta acgccgatat
     1141 ttgcaaatta taccaagtat ttaatgcgag atttgacaga tgtaagcgaa gaatatgaag
     1201 gagggaagta aagtgccaag aaaaggtttt atagcaaaaa gagaagtgct acctgatccg
     1261 atatacaata acgttgttgt aacaaagctt ataaacaaca ttatgcttga cggtaaaaag
     1321 ggtgttgctc agaaaatagt ttatgatgca ttcaatatcg tagcagaaaa gacaggtaag
     1381 gacgcacttg aagcattcca ggaagctatg gataacatca tgccggttct tgaagtaaaa
     1441 gcaagacgtg tgggtggtgc aacataccaa gttcctatgg aagttcgtcc ggaaagacgc
     1501 caaacattgg gtctaagatg gcttactcgt tactcaagag aacgtaacga aaagacaatg
     1561 aaggaaagac ttgcaaacga aatcgttgac gctatcaacg gtacaggcgg ttcagttaag
     1621 aagcgtgaag nnnnnnnnnn taaacaaatc gcttaactgt gaaatttaac ccgaaaatcg
     1681 catataatag gggacaagca gttttgcatt gcccctatgc ggatttcgac agatacacta
     1741 tttttacctt tgttgaaagg aggaaattat tttgggtaga caatattcac ttgaaaatac
     1801 aagaaatatc ggtatcatgg ctcatattga tgccggtaaa actactacaa cagaaagaat
     1861 tctgtactac acaggtatca accacaaaat cggtgaaaca cacgatggcg gtgctactat
     1921 gga
//
LOCUS       DAWACS010000279         1918 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_5979_length_1918_cov_1.703704, whole genome shotgun sequence.
ACCESSION   DAWACS010000279 DAWACS010000000
VERSION     DAWACS010000279.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5979_length_1918_cov_1.703704"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1074..1083
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          492 a          452 c          298 g          666 t
ORIGIN      
        1 tcttaattct ctatatttct taacattttt acctaataca tttgattttc catttaattt
       61 ttttactttc ataataaatt aaatcctttc tctcttttgt cctatgctca aaaataaggg
      121 cggtagtttt tcagccgccc ttttcaatta tttattaagg tgtttatata caacaacacg
      181 ttcgtcaaag tcgtgtttct ctttaccaat tatctgatat gtttcctgtc ccttacccgc
      241 aagaatgatt acatcatctt tctttgcgtg gtcaagagca tatgcgatag cctctcgtct
      301 gtctactatt actttatatt ttccgtctgt gcgtttcatt ccgacttcta tctgttctat
      361 aatagaaaca gggtcttccg tacgcggatt gtctgacgtt ataatactgt aatccgaaag
      421 ttttccggcg atttcaccca taatcggtcg cttcgtatta tctctgtcac cgccgcagcc
      481 gaacaatgtt ataacatcac cctttgcaaa atctttaacg gttttaatta tattttcaag
      541 accgtccggt gtatgtgcat agtcgattat aactgtataa tcggtatttg taggaacaac
      601 ctcaacacgt ccgacaactc ctgttgcatt ttcaagacct ttgataatgg tatccatatc
      661 aatacccatc tgcaatgccg cacccatagc gcatattgca ttatatacgc tgaacttgcc
      721 cggaattact atatgaacac gatatgtctt accgtcatat tcaacatcaa aatcagcacc
      781 cgatgatgaa atttcaatat tttttgcttt aaggtcgcaa ccgtccgaaa gaccgacttt
      841 taatatatcg cagtcattgt ttgcaacaat cttcttaccg ccgtcatcgt cgaaattaac
      901 aacaccctta tcgctgattt taaacagctt agcctttgca tttaagtaat tttccatagt
      961 cttatggaaa tcaagatggt cacgtgtaag atttgtgaac acacccacgt caaagtgaca
     1021 accgtgtact ctttcaagtt caagtgcgtg actcgaaact tccattacaa cacnnnnnnn
     1081 nnncgcaaat agctgtttaa gttcaagtgc gtttggtgtt gtcggtgttg tacttggcgt
     1141 aacaagcacc ttgtcgccga taatattttg atttgtaccg ataacaccga ttctcatacc
     1201 ggccgtttcg attatacttt ttattaaata tgttattgta gtcttaccgt ttgtacctgt
     1261 gataccgata agtttgaatt tgcttgacgg attgccgtaa tacttacaag ccatttccgc
     1321 aatttcaact ctcgaatttt caacatacca aacaggtaca tctacatcta ttttatcttc
     1381 ggcaacgatt agtgacgcac cgtttttaac tgccatttct gcatatttat gtccgtccgt
     1441 ttcaaagcct ttgatacata caaaaacgtt tcccggaagt acgtttcttg aatcgtacgc
     1501 tattccggtt atatctatat cttcatgctc atcaagccat ttactgccta ataattcact
     1561 tgctaacatt tgacatttcc ccctgtccta ttagtctgaa gttacttggc ggaattccac
     1621 gcctattact gttgcaggtg taacctcctc gcctgccgct atgctctgat ttaccgcata
     1681 cgcactcgac acttcactgt gtcccgcacc gacaatctca aagttcagtc cgctcatttc
     1741 aagagtatat ttcgcattat ccacggacat tcctttaaga ttaggtactg ttaccgtatg
     1801 tgttttgtca atttcttctg tatataatat taccgttgaa tcttcgttaa gcattgtacc
     1861 cggtttcgga agctggtcaa ccaccgtatc gccgtcacct ctcacctgaa cattcagc
//
LOCUS       DAWACS010000280         1912 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6004_length_1912_cov_1.473344, whole genome shotgun sequence.
ACCESSION   DAWACS010000280 DAWACS010000000
VERSION     DAWACS010000280.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6004_length_1912_cov_1.473344"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          485 a          460 c          312 g          655 t
ORIGIN      
        1 aaaagccttt atttccttcg gcttaggcag ttcagtagcc gtaaatccgc ccttatgctc
       61 aaattcaccg tcaagtatac tgttgcacat atctatacat tcgttacaga tatatacccc
      121 cggtcccgac aaaagcttat ctacttcttg ttcagttttt ccgcagaatg aacatctgaa
      181 atgtttattt tcgtccatat atttatactc ctttatttag aggcttttac aataacctcg
      241 tcaataaggc cgtattcctt agcctcttga gcagtcataa agttatcacg ctctgtatcc
      301 tcacaaagct tttcgtaagt ttgtcccgta cgctctgcaa gaatttcatt catcttcttc
      361 tttgtattaa caagatgatc tgtgtatatt ttaatatcag ttgtctgacc gctaagtccg
      421 ccgccggcaa taagaggttg atgtatcata acctcactgt tcggcaaagc aaatctcttg
      481 cctttttcac cggccgcaag caagaatgca cccatacttg ccgccatacc gatacaaatt
      541 gtagatacgt cacactttat atactgcatt gtgtcgtaaa ttgccatacc tgcagtaatc
      601 gagccgcccg ggctgtcaat ataaagctga atatctttat caggatcctt tgcttcaaga
      661 tataaaagct gtgccacaac aagacttgcc gttgcgtcag ttacctgatc tgccaagaat
      721 ataattctgt cctcaagcag tcttgaataa atatcgtatg aacgttcgcc tctgttggtc
      781 tgttcaacga ccattggtac taatgccata ctattcatcc ctttccgaaa ttatgaaatt
      841 attttacctg tgcattgttt acaatcatag tgattgtctt ttgaatttca aggtctttct
      901 taatgctttc cttttcagcg tcaccaagaa gttccttaag cttgtcaagt tccatattgt
      961 acttctttga catatctaca agttcatgtt caacttcttc aggacctgct tcaattcctt
     1021 caaccttttg aattgcctca agaacaagcg aaccgcttag ctgtttctta gcctgttcag
     1081 cgaattgagc tcttagagcg tccattgtca tacctgtata ttgcatatac atatcaaggt
     1141 tcataccttg gtatgaaagt ctttgagcaa aatctcttac actgttgtcg atttgagttt
     1201 caaccatagc gtcaggaaga tcgaactcgg cattttcaat aaccttgtcg ataactgcat
     1261 tttcggtttc aaccttagct ttgttttcag cagcctcttc aagctttttg cgaacgtctg
     1321 ctctgaattc gtccattgta tcaaattcgc ttacttcact tgcgaaatcg tcgtcaagct
     1381 caggaagttc acgtttcttg atttccttaa cctttacttt aaatactgcg tccttacctg
     1441 caaggttttc tgcatgatat tcttcaggga aagtaacctt aacgtcaaca tcagcgtcag
     1501 tttcagcacc tacaagctgt tcttcaaatc caggaatgaa tgtacctgaa ccgatttcaa
     1561 gttcgtagtc ttcgcccttg ccgccttcaa atgcaacatc gtctacaaag ccttcaaagt
     1621 cgataactgc gatatcgccg ttttcgattg ctctgtcgtc aactgtgata agtcttgcat
     1681 tcttcttttg tgtagcctcg atatccttat ctacatcttc atctgttaca gtatggtcaa
     1741 ttttttccac ttctatacct ttgtaatcgc caagcttaac ttccggctta gttgtaacaa
     1801 gtgctgtaaa tactacaggc tcaccctttt tgatttcttc aacgtcaatt tcaggtcttg
     1861 caactgattc aacacctgat tcctttagag cttcgtcata agcctgcgga ag
//
LOCUS       DAWACS010000281         1911 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6008_length_1911_cov_1.676724, whole genome shotgun sequence.
ACCESSION   DAWACS010000281 DAWACS010000000
VERSION     DAWACS010000281.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6008_length_1911_cov_1.676724"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          599 a          297 c          425 g          590 t
ORIGIN      
        1 ttgtataagt atgaagggcg gtatgtgact gccgtcgggc gcgtgtgcga gataccgcag
       61 gacagcgaaa aagatgaaaa tgtacgatat gtaattgatg tgcgtaaagt aaataatgaa
      121 aaagtaaggg ataaaatcct tcttacttct gataataagc cggaatacgg tgatactgta
      181 actttcagcg gatttatcaa cgaacttcct gaaaaattga atagaaatgg ctttgattat
      241 aaaaaatatt ataaaaaatt ggggattttc tttaaatcat acagcagtga agttaaactt
      301 tcggacgaag tgataaaaga ttattcacca tatgcgatta acaattcgtt taagaactca
      361 atttcaaaat taattgacaa gcattacaag ggcgattaca atgcgataat gaaggctgtg
      421 ttgatagggg ataaaaaaga attttcggac gattttgata agctacttac caaaacgggt
      481 acgaaaagat atttttattc ttcgtatctt catataatgc ttttaatgtc cttaataacg
      541 cttgttttgg gcatatttaa aaagaggaca cgcgatattt tgacggtgtt tcttcttatt
      601 atatatgctt ttgccaatgc aaacaatacc gtttcgatca gattggctgt gatgatagcg
      661 ataattatat ttaccgaaag taaattcgga cgtaaatatt atccggatat agtcggtata
      721 acgttgcttg cggtcggagt ggcaaatccg ataatgctgt ttgacgcagg ttttgtgatg
      781 tcggtgctgt cggcaattat gatatattac ttttacgact gtgtgcacgg taaacttaaa
      841 tttatacata taaaatacat tcgcagactt tttgcgatag gcataatttg tacaatcgga
      901 cttttgcctg tttcggtgta ctttttcggt gctgtttcga tatattctat tgttttgtca
      961 ataataatgt tgccttgtgt gacggtgatt atggtgcttg caccgatttt gcttgtaatg
     1021 cttgtggtat tcgacaccgc accgctgttt aaacaggcgg ttgactgcgt gctgtttata
     1081 ctaaaacttg tgccgacaat ttcatataaa ctcggtcttg tatcgcatac agtcgcaaaa
     1141 ccgaatttgt tattacttat tatatacact cttgccgttg tcggcggagt gaaatatatc
     1201 aagaagaaaa acagtcaaat gcgtattgcg atttttgttt cggcggcatt gatgacttcg
     1261 tatgtaatag gcgaagtgat tagtacaaat aatgtggaaa ttacattcgt caatgtcggt
     1321 cagggtgacg gtgcggtaat tcaagcaccg taccgattta atgtgcttat agacggcggc
     1381 ggtggtaatt cgtattttga ctataacccc ggtgaaaagg tgtaccttga ttatcttgaa
     1441 acagagggga taacaaaggc agacagtgcg tttgtaagtc attatcatca agaccatgtg
     1501 cagggaatta tagcggcaat ggagaatata aaagtaagaa atttatttct gcccgataat
     1561 atggagggca gtgaatggcg tgcggcactt gaaaataccg caagggaaaa cggcacaaca
     1621 gtgcattaca tttcacagga aacacttctt acttatagta acggaatgac aataagaatt
     1681 attcctccgt caaataagac agggttaagt gatgatgaaa atgatacttc atatgtgtat
     1741 tacgtcgagt ataacggttt ttctgcaaca tttacgggtg atatgtcagc atttgctgaa
     1801 aagaatttga ttgatgacgg aaaagcaggc aaaacgaact tgttaaaggt tgcacatcac
     1861 ggctcgaata ccgcaacgtg tgaagaatgg gttgaaaaat taactcccga a
//
LOCUS       DAWACS010000282         1910 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6011_length_1910_cov_1.899191, whole genome shotgun sequence.
ACCESSION   DAWACS010000282 DAWACS010000000
VERSION     DAWACS010000282.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6011_length_1910_cov_1.899191"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    804..813
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          645 a          334 c          442 g          479 t
ORIGIN      
        1 gaacagtatt tcagctccag ataatatagc gcctgaaaag acaacaatag atatatcaaa
       61 taacactgat tatatattac ctcctctgtc gttcacggta atgcgtgtgt atacaaactc
      121 gcagctaatt agattggctg attgccgtgc agataagggc atttttaaat atgaacttga
      181 atctggcggc gatatatcgg agtatgacat ttacacggca ttatacgata atgatggtaa
      241 actcgttaat gtgtggaaaa atgaattaag cggcacagca aaccttgatg tgggtgcaga
      301 ctatgatatt aaggtcatgg tatgggagaa aaacagcatg aagcctgtgt gcgaagctat
      361 tgaacaatct gtaaataccg ccgaaaagat aactgaatat aaatatctgt ttacagatga
      421 taaggtagtc aagacatccg gaagcgcatc aagcggtgat gtatatgaat ggacaattac
      481 atctgcaccg cagtatttct atattgataa cattgatttc gatgccgttg agtcaattac
      541 aataagatca ggttatcaat caaattcagc cgtaacatct gtatatgctt atgataacga
      601 cggaaactat gtaagcgaaa atgagctgaa aaagtttgtg acaaacccgc ccccgcttgg
      661 aaatccgatt ggtacaatcg ctgatacaaa aacagcaacg tggggatata ggaccggagt
      721 aataggcaga aacaacgtga caatttcaga gggctcaaac tataccctgt cggatacgtc
      781 aaaaattctg gatataccgt cagnnnnnnn nnntaccgtc aggaacgggt aaaaaagctc
      841 tgattgttgg tataacagga aatataggcg aaaaaggata ttttgattat atcaccgtta
      901 aatacaataa agaggttgac gcgccaatgc cagttataga gagtatcaca ggtgaaggta
      961 ttaaagattt cacagtagag ataaatcaat cagataaatc aattttaatt ccggttattc
     1021 cgggaacaaa tctcaataca ttaaaacccg aaattaagat gagcggcaac gcttctgtaa
     1081 aacttacatc gggaacatgg aaagacggac agcttacgat ttcttacgga gacgaaagcg
     1141 aagtgtggag tattaaggct gaggacagag gaaaccctgt gctgaacgga tattatgctg
     1201 atcctaacct ttgtatattt ggcgatacgt tttatctcta tcccacaacc gatggcggaa
     1261 cagggtggaa cagcagttcg tttaaggcat tttcatcaaa ggatttgata aactggaagg
     1321 acgagggtga aattctgagc cttaaagacg tttcatggtc aagcggagtt tacgcatggg
     1381 cgcctaccgc agttgaaaag gacggcaagt attattacta ctacagcgga acgcaaagta
     1441 taggcgtggc ggtttcggat agtccgacag gaccgtttat tgacaagggc gaacctcttg
     1501 taaataagaa tactgtcgga ttatcgggac agatgataga cccggcagcg tttatagatg
     1561 atgacgggca agcatatata tactggggta acggcagaat gtacatggca aagctcacgg
     1621 acgatatgat gagtattgac ggcgaaatcc atacaattac accatcgaat tttcgagagg
     1681 gcgcatttgt aataaaacgc aacggaatat attattttat gtggtctgac aatgatacag
     1741 gtgagcctac ttatgaggtg agatatggaa catcaaattc cccgtatgga ccgatacagg
     1801 gaaataccag aattttaaca tactcaaata ctgacgattc gaggataaga ggaacaggac
     1861 atcacagcgt tataaatgtt ccgggaacag acgactggta tatatgctat
//
LOCUS       DAWACS010000283         1898 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6064_length_1898_cov_2.228432, whole genome shotgun sequence.
ACCESSION   DAWACS010000283 DAWACS010000000
VERSION     DAWACS010000283.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6064_length_1898_cov_2.228432"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1189..1198
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          623 a          319 c          364 g          582 t
ORIGIN      
        1 atgttgcacc gtacagatac aaagatacac ttgtgtatac ggacaatatg acgcgtgaaa
       61 agttctgcat aatccttgca aatatgctaa aggcaaagga taaattgccg acagagcaga
      121 atgtcaaatt cagcgataca acaagagaag aagtttcgct tttgggtacg gtaggaatta
      181 taagcggtta tgaggacggt acatttaaac ctaacaattc gattacgcgt gaagaggcgt
      241 cgatgctttt gtataagaca gcacagtata tggggtataa cgatttctat gaggattaca
      301 aattgtcgaa ttataagtat gccgatgatg aagaaatagg cgagtgggca aaagaggctg
      361 tttatcagat gaacaaagcc gagataatga caggtatggg cgacgatatg ttctcaccta
      421 aatcaaatta tacaaatgaa cagtcaatct caactattat gagattatac gatttgcaga
      481 ataaacctaa atcaacacca acgccaactt tagctccgac acctgagccg acagaagtac
      541 cgacaacaga ggaaacagat gttcccgaaa cagacggcgg agaaacaaca gttcaagaaa
      601 attaacaaaa aagtatcctc aacagaggat actttttttg agagaagata tattttatta
      661 ttttaattga agtctgattc tgtcggcaac cattgcgata aattctgaat ttgtcggctt
      721 gcctttgcct gtgtggatgg tgtagccgaa aatttcattc ataacctcag gcttacctct
      781 gctccaagcg acttctattg agtgacggat tgcacgttcg acacggctgg cggttgtatt
      841 atatgccttt gcaatttcgg gataaagctg tttagtgatg aagtttaaca gatccatatt
      901 ttcaacaacc atcattattg cacttcggat atattgataa cccttaatat gtgccggaac
      961 accaagctcg tgaatgaagt ccgtaacgat agtttcaagg tctgtttctt cggctttttg
     1021 aactgttata ttgtgtgaaa gcggtattgc gctaaccata ggaacagatg ttaaatcttg
     1081 aataacttcg tatacacgtt cgttgctttg tggctttaaa agacagtagt ctattaaagg
     1141 acttactgac gcagccattt taattgctat tgatgaaacg gaataaacnn nnnnnnnnaa
     1201 actatgattt taggtttttt atcaaatgtc aaagtctgca tacgctttaa aacaccgata
     1261 ccgtccagtt tcggcattac aaggtcaaga attataatgt ctggtctggt ttgagaaata
     1321 agagtgtaag catcttcacc gtctgttgca atagccgtta ctgtcatatt aggctttgca
     1381 cctatgtatg atgataattc ctttgctaaa tcttcgttgt catctgctat tagtacagag
     1441 atattgttgt tcataagtaa gtcctcctat tgtataaagt taatatgatg tctactattt
     1501 gtaaatgttt tccattacgt aaagtatatt accataaaat gttaataaaa tcaagagatt
     1561 tttcaaaaaa attccaaaaa attttaaaat atattttttt aatgccgtaa atactgtaaa
     1621 atacaacaaa aaaagatgtc aaaacttttt gttttgacat ctcttatatg gtggtatgtt
     1681 attaatcttt gtaatataat gctcgaagtg agtttaaaat cgcaagcaat gatacaccta
     1741 catcagcgaa aattgcaagc cacattgtgc ttatgccaag tacgcttaaa atcataacaa
     1801 gaactttaac tccgatagcg aatacaatgt tttggattat aaccgacata gtttttgcgg
     1861 agattttaga tgctttggca attttggaag gttcatca
//
LOCUS       DAWACS010000284         1882 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6144_length_1882_cov_2.675424, whole genome shotgun sequence.
ACCESSION   DAWACS010000284 DAWACS010000000
VERSION     DAWACS010000284.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6144_length_1882_cov_2.675424"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          554 a          409 c          357 g          562 t
ORIGIN      
        1 cggttatacc ctgaaattag ttattacaat aaccgcaatc ttggtaggag ggcggttatt
       61 gtgcttttat agagaaaatt atcaaaaata tgataatttc taaaattgct gtgagcaatt
      121 tcttcgccat atgtagcaat atgcttactt ttcataagca ttcccccttt cataaactca
      181 gcataacagc ctgtgaatat aaaagagagt ataaccgccg gtgaaaacac cgttaagagg
      241 tcttacctat atcggaatga tccgacagtg atattatata acaaagaaat acaaaattcc
      301 acaaagtata caaaacgaaa tacaaatgta aatgagcgca caaaaaaacg gcattatcga
      361 atgccgtttt ttgtatactt aaaaacttat ctttgtacac gacctgagcc gtcgccgcct
      421 gcaagagttt caacttcttc tcttgtaaca aggttaaagt caccagggat tgtgtgctta
      481 agagctgatg ccgcaacacc aaattcaaga gcgtccttga agttcttacc gtcaacaaaa
      541 ccgcagattg taccgccggc aaagctatcg ccgccgccta cacggtcaac gattggacga
      601 attctgtatt ctcttgaatg atagaattca tcatcttcac ttgagtaaat gcaagctgac
      661 caaccgttat cagaagctga cttagaaact ctaagtgatg aaataacata cttaaatccg
      721 aacttagcaa ccatctgctt gaaaatgctt tcgtaacctg caaggttaag ttcaccgctt
      781 gttacgtctg tgttttcagg cttgaaacca agaactttat ctgcatcttc ttcgttaccg
      841 atacaaacat caacatattg cataaggttt gacataacct tttgagcctt ttcgcttgtc
      901 caaagcttct ttctgaagtt aaggtcacaa gaaactgtta caccgtgagc cttagcagct
      961 ttacaagcag cctctgtaag ttcagcggca gcgtctgaaa cagcaggtgt aatacctgtg
     1021 aagtggaacc aatcagcacc ttcaaagatt tcgtcaaaat cgaaatcaga aatgcttgct
     1081 gttgcgattg atgagttagc tctgtcatat gtaacgtttg aaggtctcat tgacgcacct
     1141 gtttcaagga agtagatacc aagtctgtcg ccgcccttag cgatgtgctt tgtttcaaca
     1201 ccgtattttc ttagtgctgc aactgcgcaa gcaccgattg ggttttcagg aacttttgta
     1261 acaaattcag cgtcgtgacc gtagtttgca agtgaaactg caacgtttgc ttcaccgccg
     1321 ccgtagttta tgtcaaactg tgtagcttga atatattttt gatttccagg ggttgaaagt
     1381 ctaagcatga tttcacccat tgtaacaact tttgccatta tataaatctc ctttaattat
     1441 aaattacttg ttaagttctg ctcttgcagc cttgatattt gcaataaatt ctttagctgt
     1501 ttcggtaatt gcgttgtagt cgcctgtctt agcacccttt gtaagtgatg aacctgcacc
     1561 tactgcaaca acgcctgcct taatccaatc agcaacgtta tccttgtcaa cgccgcctgt
     1621 aggcatcatt tgagcctgag gaagaggacc cttaatatcc tttataatct taggaccgta
     1681 tagatctgca gggaatatct taacgatgtc agcacctgcc tccatagctg tgataacttc
     1741 tgtaattgta gcacaaccag gcatataagg tactctgtaa cggttacaaa gcttagctgt
     1801 ttctgcattg aaagcagggc ttacgataaa gtttgcacct gccaagatag ccattcttgc
     1861 tgtttcagga tcaagaacag ta
//
LOCUS       DAWACS010000285         1882 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6142_length_1882_cov_3.319102, whole genome shotgun sequence.
ACCESSION   DAWACS010000285 DAWACS010000000
VERSION     DAWACS010000285.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6142_length_1882_cov_3.319102"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1657..1666
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          546 a          308 c          462 g          556 t
ORIGIN      
        1 ctgtctttat gcgataacga gtattatatg gattgcgatt ttatatattt tcggcggtat
       61 agttttgagc agtacgcata tcgttcagaa tgtttcagac actattattt cacttggtac
      121 ggaatcaagt gcggaaaatg tgcagggaag tattatgata cttattcagc ttgctttttc
      181 aatcggtgtg ttcacgtttt ggggttcagg tcctgcaacg gcggcttatt cgtatataac
      241 aagatgtttc acacgcggtg aacatacttg ggttttgagt gacggtgcgg acaagtttaa
      301 ggaaaacttt aaacagggta tggtagttgt gctgattgat gcggttttgc ttgtgttcgg
      361 acttaatgca attcttttct atcattcgct ttatgtttca acaggcagta tgctttggat
      421 gatgttgctg tatgttatga tactcgtact tgttgtttat acgatgatgc acccgtattt
      481 gtatcaaatt atggttacat ttgaatgtgg aatcggctcg ctttataaaa atgcgttgct
      541 tataacactt gcaaaattac cgggtaactt ttttacaacg cttataggag tacttatata
      601 cggtgcattg tttacattcg ttaatccgct tgcagcggca attatagtag tagttatcgg
      661 actttgcgtt acttgctatc cgccgcaatt ttatgccgca agagtaattg aacgctcaat
      721 attaaaggat atgaaagaaa aacagcctaa ggttgaatat atcggggagg aagaataatg
      781 gttttaggtg aatatgatat tgccgtaatc ggcggcggtc acgcaggctg tgaggcggct
      841 ttggcggcgg caagattggg tatgaaaacg gttatgtttt caataagcct tgacgcaata
      901 ggaaatcttc cttgtaaccc aagtataggc ggtacggcga aaggtcacct tgttcgtgag
      961 cttgacgcac tcggcggtga aatgggtaaa gcggcggacg cttcgtttat acagtcaaaa
     1021 atgcttaata aaggcaaagg ccctgccgta cattcgctta gagcacagat tgacagaaaa
     1081 aagtatcacg ttgaaatgaa aaaacgtgtt gaaaatcaag agaatttaca ggttaaacaa
     1141 gcggaaatcg taaatgtaat taccgatgaa gataataatg taacggaagt tgtaacacat
     1201 acaggcgttg aatataaggt aaaagcggtt attattgcca gcggaactta tttaaagggt
     1261 aaaattctta tcggaagtta ttcgcgtgaa agcggtcctg acggaatgtt ccctgcaaat
     1321 gaactttcgg aaaatcttcg cagattgggt attgaattaa agcgatttaa aacaggtact
     1381 ccggcaagag tgcacgcgga tacacttgac tacagtaaga tggagcttga ggagggtgac
     1441 gaaaaaatcg tgccgttctc gtttgaaacg gagaatgtcg gcaaaaatct tgttccttgc
     1501 tatcttgtgt atacaaatga aaaaactcac caaattatac acgacaatct cggtcgttcg
     1561 gcaatgtacg gcggtatggt tgagggtata ggtccgcgat actgtccgtc tatcgaagat
     1621 aaggttgtgc gtttttcggg aaagccaaga catcagnnnn nnnnnnaatt gatacaaagg
     1681 aagtatatgc acaaggcttt tcaacgagta tgcctgagga cgtacagctt gaaatgtata
     1741 gaagtgttga gggacttgaa aatgtcgaaa ttatgcgtcc tgcatatgca atagaatatg
     1801 attgctgcga tccgactcaa ctgtatgcaa cgttggaatt taagaatgtg catggacttt
     1861 acggcgcagg actgtttaac gg
//
LOCUS       DAWACS010000286         1876 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6171_length_1876_cov_3.075783, whole genome shotgun sequence.
ACCESSION   DAWACS010000286 DAWACS010000000
VERSION     DAWACS010000286.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6171_length_1876_cov_3.075783"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          505 a          480 c          288 g          603 t
ORIGIN      
        1 tcacttcaaa aattcactct attaaaatct tgtttataat accgtaccgc cgccaagaac
       61 aatatcaccg tcataaaata cgacagcctg tcccggagta accgctcttt gcggttcggg
      121 gaacatcaat ttaaccgtac cgtctttttg cgggcaaagc attgccgccg caggcggtgc
      181 ttggtaacgt acttttgcgg tgataagcat attttcttta ggaaattcac ccgacacaaa
      241 attcacatcg tatgcggtta attcatttga aaattccatt cccttttcgc cgagtgtaat
      301 cgtattgttt tccgcatcta tcttcataac aaacataggt ctgccgtatg caccgatacc
      361 ctttctctga ccgattgtat aataaatcaa acctctgtgc ttgccgataa cattgccgtt
      421 tacgtcaagt atatcgccct ctttaggctt gtaaccggaa tagtcggtta taaatttcgc
      481 ataatcatca tcttcaacaa agcatatttc ctgactgtca ggcttatgtg caacgccaag
      541 tccgagttct tccgcctttt gacgtaccca atccttatta tatccgccca aaggcattaa
      601 tgtatgtgca agctgttctt gtgtgaaatt gtacagcaca tagctctggt ctttcttaac
      661 agcctcactt gcacgaagaa tatacttgcc gtcctcacct ttttcgattt tggcatagtg
      721 acctgtcgca acgtaatcaa tacccatagc caacgctttt tttaacaatg cgtcaaactt
      781 caaatactta ttgcacgcaa tacaaggatt aggtgttctg ccgtgaatat attcatcaac
      841 aaaataatca acgaccttat ctttgaacat atccttaaaa ttcatcacat aaaaatcaat
      901 accaagctta tcacacacac gtcttgcgtc ctcaaccgca ctttccgcac aacagccctc
      961 atgcttcata ttacagctgt ccgaatacgt ccaaagacgc atagtcgcac cgattacgtg
     1021 gtatccctgc tcctttaaaa gagccgccgc aacggaactg tcaacgccac ccgacattcc
     1081 tatcaataca cttttctgtt ccaatttatg tttcctcctc agatattaaa acggtagcct
     1141 acaccccata tggtttctat ataccgctca ttttcagtat ttatcttatc acgcacacgc
     1201 tgaatatgca ccgtaacggt cgacacatca cctattgcgt ccatacccca tattctatca
     1261 aataaaacct cttttgaaaa ggtatggttc ggattttccg caagaaataa aagtaaatcg
     1321 tattcttttg cagtaagcga tatttctttt ccgtccactg taaccttatg cgcatctttg
     1381 tctatcataa gtttaccgac tgttattacg tcaccgcttt ttgatgtaag cgtatcgtat
     1441 cgcgatatat gtgccttaac tcttgcgaca agttcgttcg gtgaaaacgg tttcgtcata
     1501 taatcgtccg cacccaagcc aagtccgcgt attttatcta tatcggtatt tttcgccgat
     1561 aaaaatatta cgggcgtttg tttctttttt ctgagttccg ttataacctc aaaaccgtcc
     1621 atacccggaa gcattatatc tattattata aggtcaaaat tttccgcaag tgctttttca
     1681 agtcccgttg taccccttgc cgcaatttca cactcaaaat catttatttc aagataatct
     1741 ctttcaagtt ccgcgatact gctgtcatct tctataatca atattttttt cattttattc
     1801 tccttagatt ttcggcaaga atacaaccgc cgttgtaccc ttacccaatc cgtcactttt
     1861 cagccatatc ttgccg
//
LOCUS       DAWACS010000287         1874 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6180_length_1874_cov_2.337548, whole genome shotgun sequence.
ACCESSION   DAWACS010000287 DAWACS010000000
VERSION     DAWACS010000287.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6180_length_1874_cov_2.337548"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1624..1633
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          563 a          381 c          400 g          520 t
ORIGIN      
        1 caaacgaatg actacaatgc acagggacat aaaaggttat aaaacaataa taaagggtgc
       61 attggaattt gtacttccgc tatgcaccga tatttataac ggtcaaaagt ctattccgct
      121 tacttcacag agcaagcgaa aaattgtatc cgaaaattca aaaatgactt cggacggttt
      181 gcgtgtaatc gcggtttgtt atcgtgatga ttacacaaag acagccatca acgaaaacaa
      241 tatggtattt atcggtctta tcggcattga agacccgccg cgaaaagagg caaagcaatc
      301 cgtagaactt tgtaaaagag caggtattcg tcctattatg ataacaggcg atcacgcaga
      361 aacggcttta tctgttgcga aaaaaatcgg aattgcggac gataattcaa aggttatgac
      421 aggcgatatt cttgaaaaaa tatcggataa cgaacttgca ctcacaataa atcaatacaa
      481 cgtatttgca cgcgttactc cgagccataa aatgaaaatt gtaaaagcgc ttaaagccaa
      541 cggtgaaatt gtcgcaatga caggtgacgg tgtaaatgac gcgcctgcac tttccgccgc
      601 cgatattgga tgcagtatgg gcataacagg tacggacgtt gcaaaatcag catccgatat
      661 ggtgcttacg gacgataatt ttgctactat tatatatgca gtccgtgagg gcagaagtat
      721 tttttcaaac atcaaaaaag cggtacaatt tttgctttca agcaacatag gcgaaattct
      781 gaccgtattt tcgggtatta tgttcggttg gtcgtcaccg cttaccgcta tacagctttt
      841 atgggtaaat ctcgtcaccg actcacttcc tgctatttct ttgggacttg acactcccga
      901 cagcgatata atggataaac cgccgcgttc accgaaaaaa ggtttatttg ccgacggact
      961 ttggctgaca atcatatttg agggcttgat gataggtgca ctcgcacttt tggcattcac
     1021 cataggtgca aacatcttcg gcggtattac agcaggtcgt acaatggcat ttgcggtgct
     1081 tagtatttct cagttggtac acgcatttaa tatgagaagt gaacactccg tttttaaagc
     1141 cggtatattt aaaaatcctt accttgtgct ttcactgatt tcgggactta ttttggaggt
     1201 gtcggtaatt tccgttccgt accttgcaaa tattttcgga gttgttcctc ttaatgctat
     1261 gggctggatt gttgtaacgg tgctgtccgt tatgccgctt ataatcgttg agcttcaaaa
     1321 gtttgtcagc tcgtttgtat ggcgaaagga ctgatatatg aatttaagca atttaaagaa
     1381 aaacgaatct ggcgttatat ccgcaatacg gttaagcggt tcaattaaac gccgtctttc
     1441 cgatatggga atgacgcccg gtgcaaccgt aaaaatgctt agattagcac cgctcggcga
     1501 tcctgtcgaa tttcgtgtac tcaactgtaa cataggtata cgaaaaaagg acgccgaaaa
     1561 aatttttatt cacaaggtgg tgaaatgaat gtccgataca aaaacagtcg ttgctcttgt
     1621 cggnnnnnnn nnnccgcata ggcgttactg ttgaaagcaa aaaaggcata tgccgttaca
     1681 acaaaaatat gcttatagtc gatcttccgg gtatctattc cctttcaccg aacagtgcgg
     1741 aggaaattat aacgcacgac ttcattataa atgaaaagcc cgatttaata ctcaacatag
     1801 ttgacgcaac caacctcgaa cgcaatctct accttacaac acagcttgcc gaaacaggcg
     1861 tgcctatggt aatc
//
LOCUS       DAWACS010000288         1872 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6193_length_1872_cov_1.780407, whole genome shotgun sequence.
ACCESSION   DAWACS010000288 DAWACS010000000
VERSION     DAWACS010000288.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6193_length_1872_cov_1.780407"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          616 a          337 c          376 g          543 t
ORIGIN      
        1 caacaaagcc gtgcgataaa gatgtggaaa tattaataaa aaacagaaag gattaatttt
       61 atgaaagaat tgataaacgc aaacgataaa tacaaaatga attggattga gggaaacacc
      121 gagtggggta cggttaaatg ccataaaggc atttccgtaa aaaagacttc ctctcaatca
      181 ggcgatataa tcacagagcg atacacgttc atgaacacca ccgacaagga catttttacg
      241 tcactgaaag atatttcgat atacacacct tttaatgatg attacacctg cggtgctaaa
      301 gcgtgtatga cgacaaaatg tcacacacat atttggtgtg gcgaaaatat ttcgtatgtt
      361 atgtgtctta gaatgggtgg cgaagctcca cacctcgggc ttgtgctgac cgatggaagt
      421 ctttcaggtt acagtgtcga gcgtgatttt gaaaaaatca gcaacgacag aggtgatttt
      481 attcttcacc cgtcacctgt cgcacttgtg ccgaatgaaa gttttacgat tgaatggaaa
      541 ttattttggc ataacggcaa gaacgatttt tacagcaaag ttaatcaact atgcaatcgc
      601 tttatcaatg tttcggctga aaatttcgta ctgttcagcg gtgaaaatat caatataacc
      661 gtttcaccta actttgattt ttctgaggtg caaatcaaag aaaataacaa atctgttgac
      721 tacaaaatcg aaaacaatga aattgtcatt tcaacaaaag ccgatacacc gagaaagtat
      781 tcatattcga taaatattga cgatgttgtt actcactgca acattttggt cttgccttgt
      841 cttgatgaac ttgctaaaaa cagatgtcat tttatcgctg aaaatcagca atacaacaat
      901 ccgaaaagca agcttgacgg tgcatatctt atctatgaca atgaggaaaa gcatatatat
      961 tataatcgtg aatacgactt taacggaggt cgtgagcgtg tcggtatggg agtgcttatt
     1021 gcaaaatatc tgcaaaatca cgatgacgaa gtgcttttaa atagtcttaa aaagtacata
     1081 aaatacgtac aaaacaacct tgtttgcatc gaaacaggcg aggtgttcaa tgactacaat
     1141 tacgataatt cctttacccg cctttacaat tatccgtggt tcagcttgtt ttatattgaa
     1201 ctttacaaat tgtttgggga caaaaatatg cttgaaattg cgtataagat tatgttgttt
     1261 ttttacaatc aaggcggtga gcatttttat gctattgaag tgccgattgt aaaacttgtt
     1321 gagtgtttaa gagaaagcgg tatgactgat atggcggata atatgattgc atggtttaaa
     1381 aaacatgccg attatatcgc cgaaacagca cttgattatc ccgcacacga agtaaattat
     1441 gagaataagt atcttgacgc tgcaaaaatt caacttgatg tactcgaact ttttaacggc
     1501 ttacagcctg actgtcattt gtatgaaacc gccatacgtc attgggacgg ttattggttc
     1561 ggcaaatgtc gactgtacgg cgatactttc ccacattatt ggagtgcgct tacaggtaat
     1621 gcgtatagtg attacggcga tattataggc tcaaacgagt ataaaaaaag agcggattat
     1681 tctcaccgct ccgtgcttag tctgctgaac tccgacggca ccgccacttg tgcatacgtc
     1741 taccctacct cgataaacgg tatatccgca aactacgccg acccatatgc aaacgatcaa
     1801 gattggggat tgtattttaa cataaaatag taaaaagcta tggctaagag tattcttcaa
     1861 gtgttgtgta aa
//
LOCUS       DAWACS010000289         1871 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6198_length_1871_cov_2.103524, whole genome shotgun sequence.
ACCESSION   DAWACS010000289 DAWACS010000000
VERSION     DAWACS010000289.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6198_length_1871_cov_2.103524"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    662..671
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          473 a          379 c          382 g          627 t
ORIGIN      
        1 caaaaagtgc acaggcaagc cgtatatcga agataggctt gcctgttgct ttttatagct
       61 ataaattaaa ctttaaaata taaaaatata atatattgca cgttccggac aatttgtcga
      121 cagtctgaac ggcttacaaa ggtgagccgt tttctttatt ctcttgtaaa agttcggtat
      181 agtatttttc aagttcggga tattccaaaa gtcctttgcg tccttctgtc gttataccgt
      241 agactccttt tttgacgtta taaaaccaac cgtaaacatt cattcttaga atattatacg
      301 tgcggtcaat accgctgatt tcctttatgc gtttcggcga caaatcgccg tacatatcaa
      361 ggatacacgc gatatgtata cattgttcgg tgtaagcggt tatgattttg gtgttcgtca
      421 caccgcctgt gtttgtgtca atcgttctgc cgttgatttc tgtgagaatt tcacgccttt
      481 ttcgatagtc gcgtgttgtc ggtttgaatt cggtcggctc aaatacgatt tccgcaaatg
      541 aatgactgcc gcgaactgaa acgaatataa gtccgagttc aagttttttt atcaccgcaa
      601 atgtgctgcg gaattttttc agtgaatatt ttttcggctt gggtacggcg atatatacgt
      661 cnnnnnnnnn nacggataaa tttttcttta actcgataat aataaattcg tcatctttcg
      721 ttgcggtaac gtcacagtca cgcacctctg cattgacttt gtatccgcgt tttacgaata
      781 aatcacgtac aggaggaaaa agttcttttt ccgtcatatt tctttaagtt ctcccggttt
      841 gatttcgtaa tgctgaccat caacttcaat ccatacagat attgcgttgg ggttgtaaac
      901 tttgtctccc ttagcggtgt atttaagatt ttttgcggca ttcatatagt caacgatttc
      961 aatatttgta gcgtaccata tatcgtcacg tccgccgata aatttacaga aaccttcgat
     1021 taaatcccag ctgttgtcgc ggtcaaattc ataactgtgt ccccaaacgt acatcatata
     1081 aagatattgt gtcttatgta ggtcggcaaa acgttctgca ttctgcataa ggttgtgacc
     1141 gtggtgacag gtagatttcc attcaaggaa gttgtcggga aatgcaaaat cgtccgtact
     1201 tccgacaaca cgactgtatt ctatgccgca ggcaggaaga agattgataa tatccttgct
     1261 gtatgagccg ttagggtagc taagacctct tacgggatag ccgactgcgt cctcaagtgc
     1321 ttttctgtcc tcaattactt gctgaacaac ttggtcaagc ggacaacgtt caatagttgg
     1381 gtgagtgctt gtatggcacg atacttcgtg gcctttgtaa agttccttga cttccgaaag
     1441 ctgtattctg tcctcatcaa caggcataat acctgtgtta aggtggaatg tacccttgat
     1501 accgtatttg ttgaaaatct cgataagctt tctgtcgaaa atcttaccgt catcgtaact
     1561 cattgtaagt accttgtgtt ttccttgcgg gaaacaagtg taaattgttt tgaaaggtct
     1621 tttgttttcc attttttata tctccttttt gtgttttaat gtaatgaccg ttatttcagg
     1681 tcggttaaac aatctcggca aaagcatagg gttgcctata cctctgttta caatcatatc
     1741 ggtgtgcggt gcgtggtaat gcccggcaaa gtatttcgga aagaacattg acgatttact
     1801 gccccagctt gatttaggtg caacgacacc tctgcctttc ggtattctga attgcccgcc
     1861 gtgcatatga c
//
LOCUS       DAWACS010000290         1866 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6212_length_1866_cov_2.065157, whole genome shotgun sequence.
ACCESSION   DAWACS010000290 DAWACS010000000
VERSION     DAWACS010000290.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6212_length_1866_cov_2.065157"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1024..1033
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          633 a          282 c          401 g          540 t
ORIGIN      
        1 gtagattcat atgaaacaat ggtagtttca agtgcaacag acgaaagaat cgtaggtaaa
       61 gtagcagatg acgataactt cggcgataca aaggcgctaa caatcaaact ttacgaggca
      121 gaagacagaa caatcactgt tacaaaagca ggtaacgagc ttagacttag aaacctaaag
      181 aagaacgatg tagcaacaat cagaagaagt cttgacgata cagttgttga cgttgttgta
      241 acaggcgaaa gctttacagg ttcggcgtca ggtatttcaa agaaggttga caagagctat
      301 gctacaatca acggtacaaa gtacgatgtt gcaaacgttg cagttggcga tcttgtaagc
      361 ggtacacaat caacattcta tacagatatg ttcggtcgta tagcttatat tgaatcagca
      421 ggcggaagtg ttcttcagtc aggcgaaaag tatggttgga ttatgaacgg ctatgattca
      481 gaagatggta gcggttacat tattgaaatg atgactcaag acggtaagtc agctgaatat
      541 aagcttggtt caagtgttga ttactgggct cctacagcaa caactgctac aacactttct
      601 tcaaaagaag aggctaagtc aacaatttca gctcttgtaa gtgcaaattc atttatgaaa
      661 ctttcatctg atgcaaatgt tgcaattaga cttgtaaagt ataaagttaa ttcaagtggc
      721 aatattacaa gattatattg tgcagttaat gctaaaacag tttctgatga caaggctctt
      781 agaattaatc caacaaactt aaagagtact gcggttgtat ctggtcttgt tggcggatat
      841 aagattggtg acggtattgt tgaaatgtca gttccaaaga ctgcagcaga tatgaaaaat
      901 gcagacaact ataaggttgg tacagtaaat tcaggttctt atgtggtaag agaaaacggt
      961 tcaagtcgtg atttcgttgt tggtcaattt gaaaatacaa atgttcctac aattcttatc
     1021 aacnnnnnnn nnnctcttgc agcagtaaag gatattgata cggctgctaa caatccgaca
     1081 atggtcgtta atgaaattga aactggtgtt gatgatgatg acaacacagt ttacacaatc
     1141 aatggttact ttaatggtgc agaagtatct cttacaacaa caaagaatgc tgttttaggt
     1201 aagttgaata acaagggtaa gaataacggc attattgcag accagggtgg tagcaagttc
     1261 tattcaacaa cagatatttg gaatgcacaa tctggtgcta aattgactga ttacttatca
     1321 gagggcgatt atattcttta tgatggtgga gatagaattg tcttggtact tgacgcagat
     1381 gatgtgtata aacaagtata tgaaggtaag actgtagccg atgctctacc gtttggtgca
     1441 tataaccaat ttattgctcg taatatgatg gtatatggtg cagtgctttc atcagatctt
     1501 gatgatgagg ctaaggttga aattgatgca agtgcattga atactcttca attcgactca
     1561 tcaaagctta tggatactat tgaaatcaat ataaagaccg gtaaggcttc tattgataca
     1621 gaaggtagtg agatttcaga tcttgagcca tatgattctg aaacaaaaac aggtgattat
     1681 gcaatagcaa gattctgtga taagggtgct ctacaagaaa tcgttattta cagatttgta
     1741 aactaatttc tgaacagaat aatacaataa cggacgcgaa tgcgtccgtt atttttatgg
     1801 cgaaatacaa tataagattg caattataat acaacaatat tacattttgg ttacatttca
     1861 caaaat
//
LOCUS       DAWACS010000291         1863 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6228_length_1863_cov_1.526549, whole genome shotgun sequence.
ACCESSION   DAWACS010000291 DAWACS010000000
VERSION     DAWACS010000291.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6228_length_1863_cov_1.526549"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1189..1198
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          715 a          300 c          380 g          458 t
ORIGIN      
        1 attatgttgc gtcgataact cttccggaaa accaaagtgc agaaaaacct gttgtatatg
       61 aagtatatta tgttgttaaa aataaagaaa caaaacttaa aaataactta atagtcgatt
      121 atgataaaag tgtgtgcagt ataacggatt ttgaaataga cggtcagctt ggtcaaagta
      181 caatttatga atcggaagac agtcatacaa tcacggttta tatgccatat gatcatgagt
      241 atcaagatta ttatacgcct tcaaaactta catatatcgg cggaagaatt tcaaatgacc
      301 aaggtaaacc gatacaatat actgttgata taaacggcta tgcgagaaca aaatatacgg
      361 taactgctca agacggtaca acgactgccg attatatgat taagatatat aaagaggcaa
      421 ctccggtgat tacatctctg agccttagaa acccaacatc aagtgcggct tctaccgtta
      481 cggtaaaaat aatcggacgt gcattgagca gtataaagaa tgcggaaaac gagaataaca
      541 gaaaggtata tatttattct gatgacggac ttgatcctgt cgaggcaaca tatgaccttg
      601 taaacggtgt tgatacatat acggcagaaa tcaatattcc tgaaaataca agcgatacag
      661 aggataaaat atatacattg aaagctaaaa tcggcgatac cgaacaaaca agtgttaatt
      721 caactatacg agtcccgaga aaagagcgcg gacttatcgg tataaatgat tttacaatca
      781 ataatcaaat aggcgataca aaaatatcgg gtgaacaagg taaaaatata agcattacaa
      841 tgccttttga cgcggatatt acatcattgc tgccgaatgt tgagcttgaa gatatgtatg
      901 cgacatattc gccggctaca gagcaggact ttacatctga tgtagtttat acggttacag
      961 cggaggataa ggttacaact aaggattata ctgttcatgt tgaaaaacag gcagcacctc
     1021 aagtgaacag cataacgttt gaagacccta aacaaaactc ggaaagcaga gtacaggttc
     1081 ggattaacgg tgataatctt gataatgcgg caaatgcact aaaccatgaa aaaacaatta
     1141 ccgtaagtgc aaaacttgta agcggtgaat ttgaaggaag cggtatatnn nnnnnnnnac
     1201 aacgcttacc gttccggaaa gaaagtcgaa cagaaaagaa ttgacagatt ttgttgtttc
     1261 ggaaaaccaa agtgaaattt caagaaacgg aaataagttg tacttgtatg taccgtacaa
     1321 cacgaattta aacaatgtaa tacctaaagt aagtcataca ggtgtatcat atacacctac
     1381 agacgcacag gatttgaaca gtacaaaaga atacaccgtc attgcagagg acggaacaaa
     1441 aaatgtatat caaatcaatg tattgagaga aggcgttgca aaggtaaaca acgtaaacat
     1501 aaatcaacct aaaacattta atgatactga tataacagtt gatataacag gtcagtttat
     1561 ttaagagatg atgaggttaa ggatacaatg gaggttgttg cagtacctcg cggtgacgga
     1621 aaaactcaaa aggtaatgct tgaatatgac ggctacggag gtcatgcaat cggtaaagtt
     1681 acattacctc aaaatgatac aagtgaggat aagaaatatg actttaaaat aacgattaac
     1741 ggcagagaac agcaaatcgg tttgtcggga attgttacag taccgcgtaa agaaagctgt
     1801 agaattacag gctttagaat taacaaccag actaaggacg ccgaaatcaa tgatgacgac
     1861 aat
//
LOCUS       DAWACS010000292         1862 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6231_length_1862_cov_2.072496, whole genome shotgun sequence.
ACCESSION   DAWACS010000292 DAWACS010000000
VERSION     DAWACS010000292.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6231_length_1862_cov_2.072496"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          632 a          306 c          362 g          562 t
ORIGIN      
        1 tgccacacgt gtgtcgttaa gagaaagttt cattgtaaat tccatatctt ccgtaagaca
       61 ggttgcaccc caaccgagtt tctttaaagt gtcaagtgcg attataaagc ctgtacccga
      121 aagttcacag caaagaccga gtttgtatct tgaaagctga aatatacggt ttacactcca
      181 aaaacagaat gaataagctg ccgtaatcca agaatcgtat gggtttttgc tgtcaagaaa
      241 accttgaaca accttaaagc cttgatttgc acgttcattc atagctttta gataattttt
      301 atcaacaagg ttgtccgcgt caaataccga tatatagtcg tattgttcgt ccattttata
      361 aagcttgtcg aacatccatt caagcgcgtg tcccttacct ctaagctccg gaacgtttct
      421 tacatataca agtgcaccgg catcttcagc cgcttttgcg gtattgtcgt cgcaattatc
      481 ggcaattaca aaaatatcat atgcgtcatc gggataatca agctgtttca aagattcaac
      541 catattaccg ataacgactt cctcattatg tgcagatatt ataagtgcaa atttatgtgt
      601 tttgtcggaa attttcttat tcttgtcttt tatcggcaaa aaacccgtaa tcccgactat
      661 aagatagtaa ataccgattg acaaagaaaa tacctgtaaa attatgctaa acacagtcaa
      721 aaacatactg ccgtctgaca ttaaattacc ccctataaaa tattcatagc ctattttact
      781 atctttggct gaaaaaagca atatatttat gatatttttt gcaaattcta taattatttc
      841 taaataaata aattatatat gggtaatttt ttagtatggc ggagatgaaa ttataatata
      901 aaggcaggtg catataatag ataatactga aaagaatgct ataagctcgc ctttcagacg
      961 tgaagatttt tgacggcggg tatagtttga aaccgctata catttactgt tttttaactg
     1021 ttgtgtattc ataataaaac ttgtcctttt tgtgaaattt attctatata taaagtatat
     1081 gaaataaatt tacaatatat tccaattatt tttgcgaaaa gtgaaatatt tgtaaacata
     1141 cttaaaaagg ggtttaaaat tttagattga tatggtataa taacttcaaa aattgtgtat
     1201 tttcggcggt aaaaccgttt taagatatat agaaaggaaa aaagtgatga cattatgtgc
     1261 aaaatgtaag aaaaaccctg cggttttctt cattacaaaa atggaaaacg gaaagaccac
     1321 aagcgaagga ctttgtctgt catgtgcaaa agagttgggg attgctccga ttaatcaaat
     1381 gatagataat ttcggtgtgt ctgacgaaga acttgaaaat ataaataatc agatgtccga
     1441 atttatggag agtatggaag gtatgcctgg tatggacggc gatttgtcgg aaatgctcgg
     1501 acagatttcg ggcggtgagg acgacgaagg cggtgcggct accgctcctc ttgatgcgtt
     1561 taaagatttc tttaataatc ttggctctaa ctcaaacagt gacgaaagta ccgaggagaa
     1621 taaaaagtcg aaagacaaaa agcagtcgaa gaaaaaacag aaaaagacca tgcttgatac
     1681 ttacggcaca aatcttaccg aaaaagccgc aagaggcgag gttgacagag ttgtaaacag
     1741 agatgcggaa attgaacgtg taattcagat tttgaacaga cgttcaaaga ataatcctgt
     1801 tctattggga gagccggggg taggtaaaac tgccgttgca gagggattgg cttgcagaat
     1861 at
//
LOCUS       DAWACS010000293         1861 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6235_length_1861_cov_1.657807, whole genome shotgun sequence.
ACCESSION   DAWACS010000293 DAWACS010000000
VERSION     DAWACS010000293.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6235_length_1861_cov_1.657807"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    862..871
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1392..1401
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          560 a          284 c          415 g          582 t
ORIGIN      
        1 gttggggaga gcctgttttt attttaaaaa tgattgtttc tgtcgtgtcg aattatatat
       61 tcggactgct gatagaaaaa taccgtgata accaaaaacg taaaaaaacg tttttggttt
      121 tgtcgcttgt aattagttta ggacttttgg cggtgtttaa atatacggga tttgtgactg
      181 atatattgag agcaattccg ccgtttagct ttatgccgaa aatcaattta ccgttgccga
      241 taggaatttc attctataca tttcagacgt tatcgtatac gattgatgtt tacagagggg
      301 acactaaagc acaaaaaagc cttgtgtctt tcggaacgta tgtttcattt tttccacagc
      361 taatagcagg gccgattgta agatatacag acattgcgaa aatgcttgat gaaagacgtg
      421 aaaacatatc gcagtttgcc gacggtataa agttgtttgc agttgggttg gcgaagaaag
      481 ttcttatcgc aaatcaaatg ggtgtacttt gggatacaat cagaggaaca tcgtcaaacg
      541 gcatagtcgg tgcgtggatt ggcataattt catatgcgtt tcagatatat tttgactttt
      601 cgggatattc cgatatggct tgcggactgg gtaaaatgtt cggttttgaa tttatgaaaa
      661 actttaatta tccgtatgtt tcaaagacga taactgagtt ttggcgcaga tggcatattt
      721 cgcttagttc atggtttcgc gactatgttt atattccgct cggcggcaac aggtgcaaat
      781 taccgcgtgt gatttttaat ttgtttgcgg tttgggcatt gacaggatta tggcatggtg
      841 cgaattttaa ttttattgca tnnnnnnnnn ntccttgtgc ttgaaaaata tgtgtacggc
      901 aaatatcttg aaaaattgcc gtctttgcta aaacacatat acgcattgtt tttaatactt
      961 ctcggttgga caattttcta ttttgaagat ttcggacaat ttgcgacgta tttgacaagt
     1021 atgtttacgt tgaaaaacgg tattatcggc ggtgatacgc tcagtacaat acttatgtat
     1081 ttgccgattt tgattgtgac ggcaatggcg tcacttccga tatggaagaa cacgtatttg
     1141 aaattaaagg agaaaaagta tatgggcgtg ttggaaattg cattttgtgc ggttgtgatt
     1201 gtgctttgta cggcgtcact ggtaaatcaa agctataatc cgttcctgta tttccgtttc
     1261 taaggggtga tataatggct aaaaaaagag atatattgct tacagtattg ttttgcggat
     1321 ttataggtgc aatggcggcg cttaccgcgt ttttgccgaa acaagaggtt tcggtgaacg
     1381 aaaaacgaac annnnnnnnn ntccctgaat tcagtgtaca aaaattgttt aacggaaaat
     1441 gggaaaagga ttttgaaaca tatatttcag accactttcc cgcaagaaat ttctttgcgt
     1501 caaccgattc atattatatg ctttattcgg gacgtaacgg ctcaaatggg gtgtataaag
     1561 gaaaagacgg gtatattatt aacacacccg taaagtgtga tgaagagaaa ttaaatgcaa
     1621 atataacggc gatgaataat ttcgtgaaaa atacggggat cgaaacaaaa cttattgttg
     1681 taccgtcaac gggatatata atgtcggaag aacttccgaa agttcatacc gaatataatg
     1741 acggcgaaat aatcgacgat ataaaaaaca gggcagagaa cgcagaattt attgatatac
     1801 gcgatactct gaccgaaaac aaggacaagc agatttatta taaaaccgac caccatggga
     1861 c
//
LOCUS       DAWACS010000294         1860 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6236_length_1860_cov_2.438781, whole genome shotgun sequence.
ACCESSION   DAWACS010000294 DAWACS010000000
VERSION     DAWACS010000294.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6236_length_1860_cov_2.438781"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1439..1448
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          533 a          422 c          283 g          612 t
ORIGIN      
        1 atattgcttt catcttccgc atataagctt gccaaatata ttaacgtttt tccgtttctg
       61 aagtcgggag aactgcaaac caacgttctg aaattcttac cgtatctaat tccctccata
      121 ccactaagac tttgctccgc tacatttaat cccattagct tgcctatttc ataacatagc
      181 acctcaataa caggttcaat tccccacttc tcaggaaaat cacgaacctg taaacgtccc
      241 gatttaacaa aatacactat tccgtctttc tgatattcag ttttaagtaa attacccttt
      301 aaattactgt gtattgaaaa attgtttctt gtaatgctac tcattataat atcctccgat
      361 attgatatta tatcaaacta tatagtgtaa atggcgaaat aaaattctac cgcaaacaaa
      421 ctaaatatac aactaatata tcacaatgaa agatgaattg caaatgaaat tggtttgact
      481 aataaatacg ttcaaacgca tagacgaaga atttgctatt ctaatctcaa aaatcacgaa
      541 attaaacggt aaattataaa caagccgaat ttatcaaatc aaatcgacaa attcggctta
      601 ttttatatga atgtagctta taaactaatt actcactgat tacaggtctc atattttcga
      661 acgaatacca atatgtaata agcgaatttt tactgcgttc tgccttttgt gcctccgaca
      721 cctttgcagg tgtggttgtt aaattgacaa gtgtgccgtt atcgtcatat tccgcttcta
      781 ttttaacgca aggtctgttt gtactttctg tcagcactct tacaagctct gacacaatac
      841 ccggtgcagt tccatagccg ttaagcatta aatcgcctgc aagctgtgat cgtcccccgt
      901 tgccgtaatg gttatggtcg ccgaagcaat ccataattgc ctttgccgcc gcatcctcgt
      961 tttcataacc gttctttgca taatccgcta catattcgtt aaatgcattg tcagcgttaa
     1021 gtcctatatc aataaatcct aaaatatcag gatcatcctt tctttcctcg tagataactc
     1081 ttatccctaa agaatcatac gcatcctctc gacagccgtg gaatgtatgt gttgttttat
     1141 catatacata ttcgtagcct tcaacgcagc cgtgcggggt atagcttgta agtattactt
     1201 ttgcacccat tgcaagacac gcatcaacat aatagtcaag cggagcctta aactgcgtac
     1261 cggacattcc gcccggattt gtccccatat ttccaatggt gacaatatcg cccggtctta
     1321 tgtttatcag aatattatca agccagccct gtgtataata tgttttaaga tttcttgaac
     1381 ccttaccgtt attatgataa tcggcaagtg cggtaagctc gggataattg gcttgatcnn
     1441 nnnnnnnntc ggttatttct tctgttttta cagtatatcc cgtatgtgtt gtcccctctg
     1501 cggcaagagt tctttcatga cctgacagtg cttcacttac atattcggac accaaatcac
     1561 cactaaaagc aatctttact ctgtaatgtt ttccttttgt aaccttagct ttaaaagtga
     1621 aatcacccga caaataatca gattctgcat tatcaatact tggtgtgcct tctgcttttg
     1681 cacttccctc tattccataa ccgcgtgata cggtataagt tgttaaagga ttaatatccg
     1741 tataaccctc tgtaacactt tcatccgcac cacttccgaa gtcaaatgat aaaccgtgaa
     1801 ctgtgacctt aacacttctt gataatattt tgccgtcact gctttctcct gtggctctta
//
LOCUS       DAWACS010000295         1853 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6289_length_1853_cov_1.725806, whole genome shotgun sequence.
ACCESSION   DAWACS010000295 DAWACS010000000
VERSION     DAWACS010000295.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6289_length_1853_cov_1.725806"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    982..991
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          619 a          356 c          315 g          553 t
ORIGIN      
        1 cttttaaaga cttgagcagt attgtatctt cctgaaacat atatgcatta ttatcaagat
       61 ttttcataaa attctgaaca ggacgttcca acatttcatt gtcgatagac gacatataaa
      121 atatcgtgtt tgtatctttt tgagaatcat attcaaaaaa gtactcattt gtcgcactta
      181 atgcgatgat atactttgaa cattgatttt caagctccat aaattgcttc ttgttttcag
      241 agtcgtctaa taataatgca aatatgtgct tatcatcaag ctttacggca aacatacaaa
      301 tatatatact gtcaccgctc aagtttttgc ttttgaaata cagtcgcttg ggagtgtcgg
      361 cacttcggat tgtatcgtca aataatttgc gacattcttc ttcaactata atattatctt
      421 ggttaaaatt tccgttgttg tatgtgtcat tggtgtaata tattttgtaa tcatcatcaa
      481 cagaaaacac caccgcactg cacggcaatg aatcatatat ttcataatct tctttaagaa
      541 aatacgtcat tgtttttctc cgtttactca ttaatttgta ttttatatta taacatacac
      601 tgaatatttt tgcaattaat taataaaaaa agaacactca aaatttgagt gttctaaaat
      661 taacaaattg tcttaagcca agcctgctgc ttttagaccg atgtagattg caaataatac
      721 aactgaaatc cacataacag cgctgatgtc ctttgcctta cctgtaacaa gctttagtat
      781 tacccatgat acgataccga acataatacc gttagcgatt gaataagtaa gcggcatcat
      841 aatgattgcc aagaatgcac cgattgcatc ggcaatatca ccgtcaaact taacttgtct
      901 gattgatgaa agcatcaaca agccaacata aagcatagca ggagttgttg cgaatgaagg
      961 tattgatgta aagattggtg cnnnnnnnnn naacgtagct tgtaacagtt gatgtaccca
     1021 agcaagcacc tacaacagta cctacagcgt cagccataag agcgcctgtt gcgtttggaa
     1081 gtgaaccgtc tttatcaaga agctttcctt tttccgctac acctataagt gtacctactg
     1141 tatcaaaaat atctgtgtac aagaatgtga atacgataag tataaatgca ggaacattct
     1201 ttgctataaa tccaaagtca aattggaaca tctttggagc ctcaaaagaa cttagtgaga
     1261 attgaggaat aactgaatat acgcctgcct caggatttgg cacataccaa cctacaagct
     1321 gtgctataat gccaagaacc caagttataa gtatacctaa aagtattccg ccaggaactt
     1381 tatagtgctg taaagcaccg attataagca agcctacaag cgaaagtaca acagtcggtg
     1441 aagaaagttc accaagtgca acagttgttg attcacttgc tacaacaacg tttgcattaa
     1501 gcaatgctat gatagcaata aacagaccga tacctgctgt gatagcaaat ttaaggtttg
     1561 aaggaatctt gtttacaaga gcctctctga atttgaatag tgaaagcaac ataaatatga
     1621 taccttcaat aaatactgcg gtcaatgcaa ctttgtagct gtaacccata gcaccgcata
     1681 ctgtaaatgc aaagaatgca tttagtccca tacctgacgc aagagcaaca ggatagtttg
     1741 caaagaacgc catacataat gtagctatac ctgctgaaat tgcagttgca gagaataccg
     1801 ctccggctga cattccgtca attccaccca aatatgatgg gtttacggca aga
//
LOCUS       DAWACS010000296         1853 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6284_length_1853_cov_2.362625, whole genome shotgun sequence.
ACCESSION   DAWACS010000296 DAWACS010000000
VERSION     DAWACS010000296.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6284_length_1853_cov_2.362625"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    819..828
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          630 a          272 c          464 g          477 t
ORIGIN      
        1 tttgggcgga ggcgcttgtg tattacagga acaaggaacc gcttattctt gatgagcaga
       61 cggagaaact tgctaagaaa cttcagcgtg aggctatgga aacagacgaa cgtgagggta
      121 tggtgcgtga ataccttgaa acattgctgc ctgtaaattg gaatgaaatg tccttgtatg
      181 aacgtcggaa ttttcttgac ggaagtgaat tcggagaggt gaatattaaa ggggaaataa
      241 agcgtcagaa ggtatgcaat atggaaatat ggtgtgaatg tttcggtaag gcgaaagcgg
      301 atttgaaaag gattgacgca aatgccatat ctgccattat ggcaaaaata gagggctggc
      361 agaagatgag caaaaagagc cgtttcggaa tttacggagc ggcagcaggt tacgaaagga
      421 cagaggtata gacttctata cctgttttgt gttccgatgt aaccgaaaag ttccgtaaaa
      481 aagcttggtt cggaacactc gaaaacggca taaaacctat ataaattaaa ggtaatgttc
      541 cgatgttacc taaaaagtta tatagattat atattattat attaaaaatt acgcacatat
      601 atatacgcgc gtaggatata gggaacggaa ggaacttcgg aacttgtatg ggaggtaaaa
      661 tgcgtgagaa tgagatagaa aaaatgcttg tgaatgcagt gaaaatacac ggaggtcttg
      721 cactgaaatt tgtatcgccg aactttaacg gtatgcccga cagattgatt ttgctgcctt
      781 tcggaaaaat tgcatttgca gagatgaaag caccaggann nnnnnnnncg gaaaatgagg
      841 agcatacaga taaagcgaaa aaggcaatta gaagcattag ggtttttggt gtattgcatt
      901 gacgatacgg aacaaatcgg aggtgtgctt gatgaaattg aacagagcta atttgcatga
      961 gtatcaaaag tacggtgttg attttataat aaacaatccg atttcggcac ttatgctgga
     1021 atgcggtctt ggcaagacga taacaacact cacagctgta agtgacctca tgtatgatta
     1081 ttttgagatt tcaaaagtcc ttataatagc tcctctcaga gttggactgt cggtatggaa
     1141 acaggagtgt gacaaatggg aacagttaaa atacctgaga tgttcaatag caatcggcag
     1201 tgcggacgaa agggaaaaag ccttaaaagc agacgcagac atttatatca taaaccgtga
     1261 aaatgtagag tggcttgtaa atagcggtca cattgatttt gatatggttg tgatagacga
     1321 actcagttcg tttaaatccc atcaaagcaa acggtttaaa gcactgcgta aggtcagacc
     1381 taagtttgac agaattgtag gtctgaccgg aacacctgca cctaacggtt tgattgattt
     1441 gtgggctgag ataaatctgc tggatatggg agaaaggctc ggaagatata taacgggttt
     1501 tcgtgatgaa tatttcaaac cggataaacg aaacggtgcg attgtgtatt catacaagcc
     1561 gttggttaat gcagaaaaaa ggatatatga gaaaatatcg gatatatgta tttcgatgaa
     1621 agcaacggtt tatatcaaaa tgcctgaaag aatagacaat atttacgaag ttgagatgaa
     1681 cgataaagag atgaaacttt atcggaaact tgaaaaggaa atgtttctgc cgtttgccga
     1741 cggtgatatt gatgcagtga atgcggcgag tctcagcaat aaactgctgc agcttgcaaa
     1801 cggtgcggtt tatgatgaaa accaaaatgt gaagaaaata cataatcgta aac
//
LOCUS       DAWACS010000297         1843 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6338_length_1843_cov_1.743848, whole genome shotgun sequence.
ACCESSION   DAWACS010000297 DAWACS010000000
VERSION     DAWACS010000297.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6338_length_1843_cov_1.743848"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          613 a          355 c          360 g          515 t
ORIGIN      
        1 aaaacatcta tacggacatt gccgcaaaaa caggtgcgtc aaccgcaaca atcagtcgcg
       61 taaacagatg tataaattac ggctcagacg gctacaattt agtaattgac agaatggaga
      121 ataaagatga atagctatac cgatttcgcc tatatttacg attcgcttat gcacaaggat
      181 attgattatg aaaaatgggc ggactatata gaaaatcttt ttattatgta tgacgtaaac
      241 cccgaccttg tatgcgacct cgcatgcggt acgggcaata taacaatacc tcttgccaaa
      301 cgcggatacg atatgacagg tgttgatatt tcggaggata tgcttaacat tgcccgcgaa
      361 aaagccaacg gacttgacat actttttctg aaccaaagca tgaccgatct tgaccttttc
      421 ggcacaatgg gtgcgtttct atgtatgatt gacggtataa attacattct tccgccgaaa
      481 tcgttattaa ctctttttac caaaatcaaa acttgcttta tcgatcccga cggtcttttt
      541 atatttgata taagcacgga atataagtta aaaaacgtga taggctcaaa cacatttgtg
      601 cacagcgata aaaacatatt ttatacctgg caaaatcgct ttatcgaaaa acacaaatta
      661 tccgatatgt tccttacttt ctttgtaaag caaggcaaaa aatacagccg ttttgaagaa
      721 cgtcatctgc aaagagcata ttcggtaaat gaacttacat tacttctcaa aaaagcgggt
      781 tttaagtctg tcgatacata tgacgaactg tcgtttgata aaccgaaaaa agacagcgaa
      841 cgcatagtgt tcgtgtgtcg ttaaatactg aatatttata ggattttcct tgattttaag
      901 cccaatttgt ggtataataa ataagttaaa agtattaaat tttgtacttg gaatttacat
      961 ttcaagtctg aaaattagga ggaaattatg gcaaaaaagt ccaaagaatt gaaactggat
     1021 atggacgcaa tcaataacca acatattatt gatgttgacc ttaacagtga aatgaaaaaa
     1081 gcttatattg attatgctat gagcgttata gtaagccgtg cccttcctga cgttcgtgac
     1141 ggtatgaaac ctgttcacag acgtatatta tatgatatgt atgaggcaaa cctattatac
     1201 gaaaatgatt ttaaaaagtc tgccgcaact gtcggtgacg tgcttgctaa atatcaccct
     1261 cacggtgacg cgtccgtata tgacgcaatg gtacgtttgg cacaggattt ctcacttaga
     1321 tatccgcttg tacaaggtaa aggtaacttc ggttctgtcg acggcgaccc gcctgccgct
     1381 taccgttata ccgaggcgaa aatgtctaaa atatcggcac ttatgcttac cgatattgag
     1441 aaaaatacgg ttgactatgt acctaactat gatgacaaat taaaagagcc tgacggtctt
     1501 ccgtcaagat ttccgaattt gcttgtaaac ggctctgccg gtatcgcagt cggtatggcg
     1561 acaaatattc cgccgcacaa tcttacagaa gttgttgacg gtataatcgc agttatagac
     1621 gaccctatga taacaaccga ggaacttatg actcatattc aaggtcccga ttttccaacc
     1681 ggcggcgtta ttatgggcaa aagcggtata agaaacgctt atacaacagg tcgcggaaaa
     1741 atcattcttc gtgcgaaagc cgaaatagaa gaacacaatg accgaaacag aattgttgtc
     1801 acagaaatac cttatcaagt aaacaaagca aagcttgaaa aac
//
LOCUS       DAWACS010000298         1816 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6447_length_1816_cov_2.685406, whole genome shotgun sequence.
ACCESSION   DAWACS010000298 DAWACS010000000
VERSION     DAWACS010000298.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6447_length_1816_cov_2.685406"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    769..778
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          472 a          399 c          292 g          643 t
ORIGIN      
        1 tcataatacc gccttttgcg taccattgta tgccgagttt cggtatcggt gtattgatac
       61 cggcaatact gatagttccc gtctgcacaa tatgcggagt ttttataata ccttttatcc
      121 cgttccatac attgcttatt atactcttta ttgaattaaa gacgcctgac actgtgtttt
      181 tgactgaatt aaacacattc tgaaatgttg ttcttatact gtctattact gttttaattc
      241 cttctgtaac attcgttaca accgtcttta tattgtcaaa agcaagtcct gcggataatg
      301 ttatcgtatt ccaaatattt attataacat tcttcgcacc gtcaattatt aatgtaattc
      361 cgcttatcac attctgcaca ccgtttttta tagtttcggt attcaatgta aatataccga
      421 ctatcacttg gaacagaccg gatattgttg ttttaatgcc ttcaaatatt cctgccaccg
      481 ccgtttttat attttcaaaa acagacttta tgttatcaaa gaacatttga aaattctgct
      541 ttattccgcc tataatattc gatattaccg tttgtatatt taagacggta tcggatactt
      601 gatttttaat gccgtcaata acttttccga cagttgcttt gacgtcattt acaatctcgg
      661 aaattacatt cttaaccgga gcaagtgctg tacttaccga agtttttatt tctgcaaatt
      721 tttcagtaat tgcggttttt atcgaagtaa atttctccgc aactttgtnn nnnnnnnntt
      781 ctttattccg tttgcgacat caccgattga cgataacttt tctccgacag tttctttaat
      841 cccgttaaac ttctcactga tttttgtctt tataccgtta aatgcactta tgcatttatc
      901 cactatcggt ttcaaagcac cttcattata tgaatttctc acaaattcaa aggcttttga
      961 aaaggcactg ccaaatacat cttttatgcc tgataatttt tctttgacag tattcagtat
     1021 aggagaaagt ttttttttta cactctcgac aacctgtccg agttttccgc ctgtaagaga
     1081 gtttattgca tcatagccgg tcttgtaata ttcttttatt gctgtcatag taccggcgac
     1141 tgcacctttt attcctccgc cgtgttcctg ataggaattc ttgatgttat tcagttttgt
     1201 tgataccgta tttttaatac cgtcccaaac atttgctgca gtttccttga ctttgcccca
     1261 agcatttgaa actgtatctt ttatcccgtt gaacacattc tttataccgt tccagagatt
     1321 ttttgctgtt tcctttacct tgtcccaatg tttaaagagc agaacaccaa ccgcaataac
     1381 cgcaccgata gcaataacaa caagacctat cggtgatgtg acaaacgtca ttattgcacc
     1441 gaacactccc gtcaccacat taagtgcctg agttgcggca atttgagcga tagtcgcagt
     1501 tgtaagcggt gcaaaagcag ccgcctgtgt tcctgtcatg acagcagaaa atgcaataag
     1561 tccgtttctt atcccctcga ctacgtttgc agccgcaacg gcaattttat atgcggttac
     1621 cgctccggca attccggcaa tcacaggtga aagtgtgctt gcagcggata ttacacctga
     1681 agctatattc atcactgccg caagtgaatt gcacaaattc ggtactacta catttgcaag
     1741 tgcctgaatt aatgcagtac catttccgct gaatgcgttt acaatgctgt tcctgacatt
     1801 atcaaacgca ttatgc
//
LOCUS       DAWACS010000299         1815 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6454_length_1815_cov_2.325000, whole genome shotgun sequence.
ACCESSION   DAWACS010000299 DAWACS010000000
VERSION     DAWACS010000299.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6454_length_1815_cov_2.325000"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          513 a          388 c          318 g          596 t
ORIGIN      
        1 tttcataggt attccgagag aatgtgcaag tgcgttacgc ttttcaccgc tgccctctcc
       61 ccccgaaaga cctgctttat acaaatcggc ttttgtgatt tcgcgtccct cttttaccgc
      121 cgtcgcactt ccgtcaatcg tgcaacccgc ttttttgagg gaatcaagga ttatatcgtc
      181 cttaatcccc tcaacaccca aaaggccctc ttttccgggt tctctcttgc gtttttcctt
      241 gccgtgaaca tcgggtacat aagcgtgttt taccttttct ttcggcaaag actgttttat
      301 gtagtttctt attttaaagc cggcactgtc ggaatcggtc aaaataacaa ttcccgtttc
      361 gtccgccatc ttttttatgc tttccaatat tctgtcatca gaaaatacgc aaaatccgtt
      421 tgtcactatt atcaccgcgt ctataaaacg tgacagcttt attttgtcgt atatcccctc
      481 gacaacaatc gtttctttta ttttgtacat aataaataat attcctttgt aaattatatt
      541 tcgatattta ttttatgcac tttgtcttgc tctgtcaata ctttctatta tataattttc
      601 cacaatgtca cgaagtccct gcggttcgat acgtttcttt ataatagtat cagaaaattc
      661 cgttgcgtct ttgtatcggt aaaaaatatt atttatctgc ttgctttcca ctattttacc
      721 tccgcctgca tacacggttg tcttttctat cttaattccg tagcacttta aatcacaata
      781 atctgccgat atgctctcgg gtataatata ataatccaaa caaatgtaaa cctcactttc
      841 cttttttaaa cttttgtaca aaatctcatt atccatatta attcctccgt ctttttgata
      901 tttatgcctc ctaaccaagc cacctaacca ttatatgcat aaaaattttt gaatattcta
      961 aaaaaaattt aaaataggta ttgcagtttt tgaaaaaatg tattataata ttatagctaa
     1021 ctgaagattt gattggcgat gacggccgag cccgtgcgat tgaaacccgt gaaccttgtc
     1081 agatggggaa ccaagcagca ataagcggtg ctttcaatgt gttacggtaa actcggtcgg
     1141 agcctatttt gattattaca acgcctatat ggcgtttttt cgtataaaga aaggtattgg
     1201 agattatatg gcacatcaag ctatttacag aaaatggcgt cctatggtgt ttgaggacat
     1261 tgtcggtcaa ggccatatta cacaaacttt aaaaaatcag attttaaacg acaaaatcgg
     1321 tcatgcttac ctattctgcg gaacgcgcgg aacaggtaaa acaacttgtg caaaggtttt
     1381 gtcgcgtgct gtaaactgtc ttaatccgca tgacggaagt ccttgcaatg aatgtgaagt
     1441 atgcaaaggc attttggacg gcagtatttt ggacgttaaa gaaattgacg cggcgtcaaa
     1501 caacggtgtc gacaatatcc gcgaaatccg tgacgatgtg cgttatgttt ccacaaacgc
     1561 aaaatacacg gtttacatca tagacgaggt gcatatgctt tcaaccggtg cgtttaatgc
     1621 ccttttgaaa acacttgaag aacctcctga gcacgttata tttattcttg caacgactga
     1681 ggctcacaaa gtacctcaaa cgattttatc aagatgccaa agatttgatt ttaaacgcat
     1741 aaagccgtcg gatattatac ttcgtatgaa agaaatcgca cacggcgaca agcttaacat
     1801 atcggaggac gcata
//
LOCUS       DAWACS010000300         1807 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6493_length_1807_cov_2.009132, whole genome shotgun sequence.
ACCESSION   DAWACS010000300 DAWACS010000000
VERSION     DAWACS010000300.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6493_length_1807_cov_2.009132"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          424 a          469 c          283 g          631 t
ORIGIN      
        1 gtatcaatta tcctccttaa ttaaagaact tccggagcca atgatagtat cttcatgtac
       61 tctttatctc taagctctct tgcaacaggg ccaaaaatac gagtacctct tgggtttttg
      121 tcatctttta cgataactgc tgcgttctca tcaaatctga tgtatgaacc gtcagcacgc
      181 tttgactctt taaccgttct gactacaaca gcttttacaa cgtcaccttt ttttacaacg
      241 ccgcccggtg ttgccttctt gacactgcaa atgatttcgt cgccaactgc tgcatatctt
      301 ttcttactgc cgcccataac tcggatacac ataacttcct ttgcgccagt gttatcagcc
      361 acttttaaaa gtgtttgttg ttgaatcatt aaaacttcct ccttacgctt cacaaatcgc
      421 aagcgatttg cttactacaa cttgcaaagc aagttgttcc actctttccg ggaacgctat
      481 attacttaac tttttctact atttcaacga gtctccatct cttgtccttt gatagaggtc
      541 ttgtttccat aacctttacc ttatcggcga tagagcatac gttttcttca tcatgtgcct
      601 ttagtttgta agttctctta acgatcttac cataaagcgg atgcttttta ttttcttcaa
      661 tagcaacaac gattgtctta tccatcttat tgctgattac tttaccgatc ttaaccttac
      721 ggctgtttct ttcttccaca gataagtgcc tccttccgca cgtttctaat aatcttctat
      781 ctgctcaatt tgtacctgtc aaacaagtac cggcattaca tcgcactgcc ttgtagttct
      841 ctttcatgaa tgatggttaa aatacgagcg atagtcttct ttacatcacc aattctcttt
      901 ggattttcta attggttaat agcattctga aatctcaagt tgaaaagctc ttgcttgctt
      961 tctgcaagtt tttcttccaa ttcagctgtt gataaatctc ttagctcatt tactttcatt
     1021 cttattcacc atcctctgct tgacgtttta caaacttaca cttgattggt agtttgtgca
     1081 tagcaagacg catagcctct cttgctactt cttcacttac gcctgccatt tcaaaaagca
     1141 ctctgccagg cttaactact gctacccaat actctggagc acccttacct gaacccattc
     1201 gagtttcagc aggttttctt gttaccggct tatcagggaa tatcttaatc caaacctgac
     1261 cgccacgctt tgtatatctt gtcatagcta tacgggctgc ttcaatctgt cttgaagtaa
     1321 tccatgccgg ctcaagagcc tgaaggccga actcgccgtg tgatacaacg ttaccacgtg
     1381 ttgccttacc cttcattctg ccacgcataa ctcttctgta tttaactctc tttggtaaca
     1441 acatgattat ctgcctccct tcggtcttct atcgccgcgt cttctgttat cgcgtctgtt
     1501 attgtcagcc gcttgttgag cagcttcctt tcttctgccg tcagcaacgt ttaggatttc
     1561 acctttgtaa atccaaacct taacaccaag aataccgtat gttgtatgag cttcagcgaa
     1621 accatagtca atatcagctc tcaatgtctg tagaggaatt gtaccctcgt gatattgttc
     1681 tgttcttgcg atttcagcac cgcctacacg acctgcaact gcagtcttga tacccttaac
     1741 gccaagacgc attgctctgc ccataacttg cttcattgca cgtctgaatg aaattcttct
     1801 ttcaagc
//
LOCUS       DAWACS010000301         1800 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6523_length_1800_cov_1.755301, whole genome shotgun sequence.
ACCESSION   DAWACS010000301 DAWACS010000000
VERSION     DAWACS010000301.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6523_length_1800_cov_1.755301"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    352..361
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          622 a          350 c          304 g          514 t
ORIGIN      
        1 gtatcacttg ataacacatt aacaggactc aaacaaggca aagtgttaca aaccattcgc
       61 agacttgtag gttcaaaaac aggtaagcat aaggtaaaga cactttcagg tcaggctaaa
      121 aaggattatt attcaatccc tataaccaag cgtttaacaa agcacgatat tgagcaaggt
      181 acaacagcaa tttcaaatct cggttcagtt taccgtgagc aaaagggctt gtgcgctctt
      241 cttgaaatca tacctccgca aacaactgcc tttgcaatta acgcggcaca aaaaaagcca
      301 attgttgtaa cagatgaaaa cggaaatgac aaaattgaaa ttcgactcgt tnnnnnnnnn
      361 naatagacca cagggcactt gactatggcg atgtagttcc gtttatgaga aaacttgacg
      421 aaatatttga taaccctgaa ataattcaga gctggaaata attagatata atattaactg
      481 gtagacttta ccaaaaaaga gctgtttcgt ttgaaacagc tcttttttgg tgcgggtaac
      541 aggacttgaa cctgcacggt cgcccacaag atcctaaatc ttgcgtgtct gccaattcca
      601 ccatacccgc gtatttaagt cttgcatata gtaacataaa gaataaatat tttcaatacc
      661 tttttgtcgt tttttaattt ttttcataat atataatacg gttgttaaac cgaatatatg
      721 aaaggaattg acatttatga gttgcaataa cagaaacatc agaaatatag acacaagcat
      781 atacacccaa acaagcagtt gctgccaaga cgattgcaaa gtaagaatcg tagagcgcat
      841 agtaccttat tatgtttgtc ctcctcgtcc gcagcctcct tttccaccat catatgcaca
      901 attcatcaat aattcagcca ccggagcaac atttgctgcc ggtgaaaaca ttgcatttcc
      961 aatcgagcaa ttaaacaccg ataaagctga tataattaac aataacggtg taatcacact
     1021 ttcaggcggt agaaacggaa gagcatatct tatcagttac caagttacag gtacaaataa
     1081 tgctgctact atcggaattg ccataaacgg ttcggtagac accaacacac aaaatgtatt
     1141 aaacggtgac acagcaactg cacaaggaaa atatatcgtt tatgtgccgg caaatacaac
     1201 atcaaccgtt tctgtaagag ttgtttcggg aaccataaca ggtgcatctc ctactgtcgg
     1261 aacaaatctg tctgttatca gaatagcttg atttttaata ggtatggtta atccatacct
     1321 tacataaaat catttggctt taacaatgtg gcggtatgcg ttatagctaa tcggttgaac
     1381 tgaaatatca ttatcaatgt tataaaattc cttatacctg ttttcccaaa taccatacca
     1441 cctaaacaat cgcggtgtag ttccctctgt ataagattca agttcttttg gaaaatgcgg
     1501 aagatataca gttttatttc cactttcaat ttgcgaaatg ataaaactgt ttctttcatc
     1561 ttcaaagtgc tttatttgtg tatatttaac agcataaaat acgcaacttg cacacaaggc
     1621 gataataact aaaatataaa gacctttaat cacatttttg tcatgtacaa agttttgaat
     1681 aataaatatt aacaattcac agctatatat tatcatcata acatatgccg aataaaagca
     1741 tctgccggtt acgggtgtaa caatgagcag aggtgtaaca ttcacgcatg cagcgaatac
//
LOCUS       DAWACS010000302         1793 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6549_length_1793_cov_2.476410, whole genome shotgun sequence.
ACCESSION   DAWACS010000302 DAWACS010000000
VERSION     DAWACS010000302.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6549_length_1793_cov_2.476410"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          595 a          298 c          379 g          521 t
ORIGIN      
        1 cgggagaaaa atcctcttga gaatataatg tcaaagatag ttgacctcga ccgtgaaatt
       61 gatgaggaca ttgacgcact tgtcgatttt aaagcagaag tatgggaaaa gcttgataaa
      121 atcgcagatg aaagatataa acggattttg tggcttagat atgccgaccg taaaacgtgg
      181 agatatattg cgttggaact taattttaca atacggtata ttcataaaat gcacttgaaa
      241 gctttggctg aacttgataa aatcatataa aaagaaaatt ggacactata gttcacgcaa
      301 gttcacttca gttcactctt gaacatcgta aaaaagtgtg ttatgctata atcgcgagag
      361 aagaataaac agagagatga tatgtaacag tcggctttgt gtattgttcc aataattgag
      421 gttgtattat tccgataata cggtattgca gggaagatta gaaataaata tttaagactt
      481 gaaagtgaaa agttatacaa agaaggatgt ttcctatacg gagcgtcctt ttttcgttgg
      541 aggaaagaat atgccataca gaccaaagaa accatgcaga catccgggct gtgcaaattt
      601 aactgacgga agatattgtg aggaacatat atcatgtcac cctgaagtta ctcgctcagc
      661 cacaaagcgt ggatacggaa gtaaatggag aacatcaagt aaggcatatc ttagagaaca
      721 tcctctgtgc gagatttgta agaggaacgg gaaatacgtt caagctacgg tggttgacca
      781 tatcaaaccg cacagaggtg ataataaatt gttttgggat aaaagcaact ggcagagcct
      841 ttgtaagagc tgtcatgata aaaaaacggg acggtttgac agtaaaccta catacagtta
      901 ctgaaatcgt cccgatattt atttgtcgaa caaatcgctg tgtgttcctg ttctttgcag
      961 tgtaagaaca agaatgcttt tttcaatgcg atacatcaac agccaatccg gttgaatatg
     1021 acattcacgg taatccttga gattgcctgt taactgatgg tcattgtatt cttccggcaa
     1081 tgtctgtcct gcggcaagca tacgaattac atcgtcaaga agctcaatgt ttaggttgcg
     1141 cttgattgcg agtttgtagt cttttttaaa tctggaagtc cagatgactt ttagattcat
     1201 gacttcaact cctttagagc gtcttcaaca tcatatgcct ttaaattggg atcgcgcagc
     1261 atacgctcgg tttcaagcat tgccgccact gtttctgagt tgggtgtgtt tatagttatg
     1321 tcgaacggaa tacgtccttc gcgtactgac tgacgcagaa aaatgttaaa cgctgtggtc
     1381 atattcatac caagctgaga aaacagtgta tcggcttgag ctttcaagtc gctgtcaatg
     1441 cgaaaactta catttgatat gttagccatg taaaatcact ccttccgttg attgtagtat
     1501 tattataaca tattagactg ctaatatcaa gctaataaaa cgcaatcagc agtttaatat
     1561 ataaatatta tatgcaatta taataaaaat ctcataggca gcgaagcaaa taaaaaatat
     1621 ataatcggtt aaaggtttac aatcccgaat gaacaaataa tcgggcattt tatgggtaca
     1681 ctctccccaa ggggttatat aatctatagt ggtacctaag aaatggacaa ataacacagg
     1741 gggtattata ataatattac agtgcaattt gttatggagg tatcatgatg gga
//
LOCUS       DAWACS010000303         1783 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6596_length_1783_cov_2.174190, whole genome shotgun sequence.
ACCESSION   DAWACS010000303 DAWACS010000000
VERSION     DAWACS010000303.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6596_length_1783_cov_2.174190"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          476 a          429 c          302 g          576 t
ORIGIN      
        1 acttgacgca tacgcttttt accctctttt tgtttttcaa gaagtttctt tttacgcgta
       61 atatcaccgc cgtaacattt tgcaagcacg tccttacgca ttgctctaac agtttcacga
      121 gcgataatct ttgaaccgac tgccgcttga ataggtactt caaaaagctg acgcggtatt
      181 gcctctttaa gtttttcagc catttttctg ccgcgtgaat atgcactgtc cttatgaaca
      241 ataaacgaaa gtgcatcaac catttcaccg ttaagcagaa tatcaagttt aacaaggtta
      301 ctgcgtgtat atccgcaaag ttcatagtca aatgacgcat aacctttagt tcttgatttt
      361 aacgcgtcaa aaaaatcgta tataatttca ttaagcggaa gttcatagaa tatctccgct
      421 cttgacgcgt ccatatacgt catatcctta tatattccgc gtctttcctg acataattcc
      481 attacattgc cgacatagtc tttaggtacc ataatcgtag ccttaaccat aggttcttcc
      541 atataatcaa tctctgcagg atcgggaaga tttgtagggt tatccaccca caccatttca
      601 ccgttcgttt tataaacctt atatataacg ctcggtgctg ttgttacaag gtcaagatta
      661 tattctcttt caagtctttc ctgtataatc tccatatgaa gaagtcccaa aaatccgcat
      721 ctgaaaccaa aaccaagtgc aacagatgtt tccggttcaa acataagtgc cgcatcatta
      781 agctgtagtt tagacaatgc gtcctttaaa tcttcatact gtgcaccgtc ggcaggatag
      841 ataccgctgt acaccatagg attaaccttt ttatatcccg gcaacggctc tgccgccgca
      901 tttttaacag ttgtaatcgt atcaccgaca cgcgtatctt gaatattttt aatacttgcc
      961 gcaatatatc cgacatcgcc tgccgccaat cccttattag gcacaagtcc ggcagggtgc
     1021 ataacgccga cttcaacaac gtcaaattca gcacctgtcg ccatcattct tattctgtca
     1081 ccgggtttaa ctgttccctc tttaattctt acataaacaa taacgccttt atagctgtca
     1141 taataagagt caaatataag tgcctttagc ggtgcatttt catcacctgt cggtgacgga
     1201 attttttcga caatttcttc aagtacagat tccacgttaa gacctgtctt tgccgatact
     1261 tgaggcgcgt cctccgcagg gattcctata acatcctcga tttcgtttat taccatctcg
     1321 ggttgtgcag acggcaagtc tattttgttg attaccggta caacctcaag gtcatggtcg
     1381 attgcaagat atgtgtttgc aagtgtttgt gcctcgatac cctgtgacgc gtcaacaata
     1441 agtatcgctc cctcacacgc cgcaagcgtg aaacttcata gttaaagtca acgtgtcccg
     1501 gtgtgtcaat taagttaaga gtataaattt ctccgtcatc acgcttatac tttagtgtaa
     1561 cggcgtgtgc cttaatcgtg atacctctct cacgctccaa atccatatta tcaaggagct
     1621 gttcctccat atcacgttcc gaaacttctc ccgtaagttc aagcaatctg tcagcaagtg
     1681 ttgacttacc gtgatctata tgtgctacta tacaaaaatt tctgatttta ctctgtcttt
     1741 cgtttgacat attttctctc ctaaaatatt cgataattat ttt
//
LOCUS       DAWACS010000304         1779 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6606_length_1779_cov_2.652552, whole genome shotgun sequence.
ACCESSION   DAWACS010000304 DAWACS010000000
VERSION     DAWACS010000304.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6606_length_1779_cov_2.652552"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          601 a          290 c          423 g          465 t
ORIGIN      
        1 gtgctatgga atatcctgaa aatattgaag aaaaacttag tgcaaagatt aatttgcagg
       61 ctgttgacgc actttctctt gcggaaaagg caggtacaat caaggctgta aacgtagttt
      121 taatcggact tttggcaaag agtatggacg tagataaaaa tgtttggatt gacgttataa
      181 aggaaactgt tccggaaaaa ttccttgatg tcaatattaa agcgtttgaa cttggatatg
      241 aactttaagg agtaaaaagt gcaattattg attaaaactc tacatgattt attcactacc
      301 tatataggtt ggacagtcgc aattattgtt gtttgggctg ttatagcggc agtgcttata
      361 agaaacaaaa agaaaaaata aaggagatgt acccaatggg aatgtatcag ccggaaattg
      421 aaacaatgga cagaaacgag cttgaaaaac ttcagcttga aagactccaa aaacaagtaa
      481 agaatgttta tgaaaatgtt ccgatgtaca gagaaagaat ggataaaaag ggtgtaaagc
      541 ctgaggatat aaaagaactt aaagatcttg caaagttgcc ttttacaaca aagcaggact
      601 tgagagatta ttatccgttt gagctttttg cggcagacaa aaaggatatt gtaagaatac
      661 acgcatcgtc gggtacaaca ggcagacaga ttgtagccgg ttatacaaga aacgaccttg
      721 atatgtgggc agactgtatg gcaagacaga ttgcggcagc aggcggcgat gaaaattcgg
      781 ttgtccaggt gtcatacggt tacggcttgt tcacaggcgg tttgggtgca cacggcggtt
      841 ctgaaagaat cggtgctatg acaatcccta catcatcggg taacaccgaa agacaaatca
      901 gacttatgat tgaactcggt gcaactcacc tttgctgtac tccgtcatac gcaatgtatc
      961 ttggcgaaac aataaacgaa atgggcgtta aagaccaact tagtctaaaa gcaggtatat
     1021 tcggtgcaga gccttggacg gaagatatgc gtcatcagat tgagcagtcg cttggtataa
     1081 aggcatatga cgtatacgga cttaccgaaa tcacaggccc tggtgtatct tatgaatgtt
     1141 ctgctcaaaa tggtatgcac gtttgtgaag atatgtttat tcctgaaatt atcgaccctg
     1201 atacggaaga agtacttcct ccgggctcat tcggcgaact tgttttcaca acgattacaa
     1261 aagagggtat gccaatgatg cgttacagaa cgcgtgactt atgcgtactt gattatgaaa
     1321 aatgcgaatg tggaagaaca cttgtaagaa tgaagaaacc tgcaggaaga accgacgata
     1381 tgcttattat aagaggtgta aacgtattcc cgtcacagat tgaagaagta cttttgaaaa
     1441 tcggcggcga tattactcct aactatcaga ttattgtcgg cagagaaaat aacacagata
     1501 ctcttgaagt acaagttgaa atgtcggagg gcatgctttc tgatgatgtt aagtctgttg
     1561 cggcgattga aaagaatatc gttgcaaaat tacgcagtac gttgggactt ggcgcaaagg
     1621 taacgcttgt tgagccaaaa accatcacaa gaagtgaggg taaggcaaag agagttatag
     1681 ataaccgtaa acttcacgat taatagggag gtaaaagcta tgaaactgat taaacaaata
     1741 tctgtttttg tggaaaataa atcgggcaga ctttcggat
//
LOCUS       DAWACS010000305         1778 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6613_length_1778_cov_2.811376, whole genome shotgun sequence.
ACCESSION   DAWACS010000305 DAWACS010000000
VERSION     DAWACS010000305.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6613_length_1778_cov_2.811376"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          599 a          285 c          443 g          451 t
ORIGIN      
        1 atcgtaaatg cagataatca agttacggca gatataggca agagtgcaaa cggtgcggtt
       61 aagtatttct caagaaacgg tatggctgac gtatatcttg acggaaatat tataaaaaga
      121 ggaatagtag aaatacttaa tatcaaggac attaaaatac ctggtatgca caatgtcgaa
      181 aattatatgg ctgctattgc cgcagtaagc ggtttggtga gcaaggacgt tatagtgaac
      241 gttgcaaaaa ctttcggtgg tgtggaacac agaattgagc ttgtaagaac acttgacgga
      301 gttaagtatt ataacagctc aatagattca agccctaaca gaacgataaa cactctaaga
      361 gtatttccta ataaggttat aatgatagcc ggcggtaagg ataagggtat tccttatgac
      421 gaaataggac ctgcacttgc cgaacacgtt aaggtgctta tattaatcgg tgcaacatcg
      481 gataaaattc aagaggcgct tgacgctgaa ataaataaga ccggaaacgg taaagatata
      541 gaggtaattc gtgcaacgtc ttatgaggat gcagttaata cagcaagaag taaggcacat
      601 gacggtgacg ttgttcttct gtcaccggca agtacaagtt ttgatatgtt cagaaacttt
      661 gaagaaagag gaaatctatt caagaaaata gtaaatgagt tgaattaata tgagaaaact
      721 tataagaaaa ttaagtgata tgcgtggtat atcgggattt gaatatcgtt tgacagacca
      781 aattgccgat atgtttaaaa agtattgtga tgatgtaaaa atcgattctt taggcagtgt
      841 gattgcggta aaacgcagta ataaacctaa cgcacctaaa ttgatgattg aggcacattg
      901 cgacgaaata ggattgattg tcacatcaat tacggatgag ggatatttga cattcgcgaa
      961 tgtcggcggt gttgacgaaa gaacacttcc gtcaaccgaa gtgactgtac acggcaaaga
     1021 agatttgtgg ggagtaatag gcataaagcc gaattactta ctggagcaag gaaagacagc
     1081 caaattaaaa gaattggcgg ttgatacagg tttgagtgcc gaaaaagtta aagaacttgt
     1141 ggctgtcggc gacagtataa ctcttgcaca gtctgtaggc agactcggca aaaagcaatt
     1201 cagcggtaaa tcgcttgacg acagagcaag cgttgcggct ataatgcgtg ttatgaaaaa
     1261 tatcaaagat ttggatattg acattgatgt gtacgccgtt gcggcagtgc aggaggaagt
     1321 cggctgcaga ggcggtaaaa ccactgcata cggcattaat ccggatatgg ctgttgcgat
     1381 tgatgtgtgt cacggtataa cgcctgacaa ttcggataat gcgtttgaag tcggaacggg
     1441 tactgttctt tcggtaggtc ctaatctcca cccaaaactt accgatgaac tttttgcggt
     1501 atcaaaacgt cacggaataa aggtaagcac ggacgttgac ggcggcaata caggtacgga
     1561 cgcttgggag atacaagttg cgtgtgacgg tgtgccgacg gcacttatat caataccgct
     1621 taaatatatg cacacatcag ttgaaacttt ggcggtttca gatgttaaag cgacagttaa
     1681 tctgctgact gaatttataa aagaacagag gggagaattg caatggctga acttctgaaa
     1741 aaaatgtctg aaataaatgc cgtaagcgga aatgaaaa
//
LOCUS       DAWACS010000306         1778 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6620_length_1778_cov_1.528149, whole genome shotgun sequence.
ACCESSION   DAWACS010000306 DAWACS010000000
VERSION     DAWACS010000306.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6620_length_1778_cov_1.528149"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    251..260
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1450..1459
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          472 a          402 c          278 g          606 t
ORIGIN      
        1 tatacctgtc ttcttccatt tgaataactt cttttcaagg aaagaaagta ggaagtaaat
       61 aacaaggtat tcggcagcaa taagaaacat tgccataaac atatccgaaa ctctgaacca
      121 gtttttagag tctactatga tatatcctaa ccctatatcc gaacccatca tttctgcaac
      181 taccaaaccc gaaaatgcaa gtgccaggct tgttttcata cctgaaatga tactcggaaa
      241 tgccgcttta nnnnnnnnnn ccctcttgcg tatatggtca agcgccgaaa aaggctcgtc
      301 cattaacaat attctcggat tatttgcaag tgctcttgct attccgactc tctgtctcgt
      361 accgcctgaa aggctgtccg gataataatc cttatatttt tcaagtccta tcattttgat
      421 atacttcatt gctatttctt ctgcattcgg gtcttttcta accgatggac caaatgctac
      481 attctgcaat actgtgtacc acggatataa cgcgtattcc tgaaatacaa tccccctgtc
      541 agccgaaggt ccgtgtatca tatctttatc gagatacaag gctccgtaat ccggtacagt
      601 gtatccggca agaagattta gaagtgtact tttaccgcat ccgcttttac ccaagataca
      661 gacaaattcg ttatcttcga ttttaagact tatgtcttca agcactctct ttcttccgcc
      721 actactctgt ggaaattctt ttcttacctt atcaatcctt agcatttgac tcacctcatc
      781 ttttaataca aaaagcgatg gctgcgtttt tttatgacgc ctttgccatc gctttctctt
      841 tgtttatttg ttaaacttat tatagcagtt gaactcatta tgttatagta tactatgcac
      901 aaaaaccgac actttattta aacatttcat acaatgataa atgttttgtc attgacggta
      961 gatttctttt ttgatacaca tcggtaataa ttcgcatata ataaatttct cacattgtgt
     1021 cgtataattc aatctaaatc atatatatgt tcacttttat aaaaaaataa ccgtagcata
     1081 agctacggtt atctgcttta aattattact ttgaagccaa gaagtcatta atcttcttta
     1141 caagttcgtc agcgtcaacg ccgtgaacca tgcaagcttc ctcaagcgat tcaccctgtg
     1201 atgccgggca gccaaggcag tgcattccga tacccattag tattggagcg atttcttcat
     1261 ctatgcttaa agcctcaccg attagagtct tttttgttac ttgtgccatt attgtttcct
     1321 cctttttata tattaaaaca ttcttcaaat tcttgaccgt caatagtttc cttttcaaga
     1381 agtttttcag ccactcgtgt aagtacagcc atattttcag taagaatttg ttcagttttc
     1441 cggtattgtn nnnnnnnnnt ccgcgtcaat atctgcggct gttttttcgg aatatgcttt
     1501 tgagtgtgca aaatcacgtc cgataaacac ttcaccgccg tcatcatacg atacagggcc
     1561 gattgcctct gacataccgt attttgtaac catttgacgt gctatgtcag ttgcacgctg
     1621 aatgtcactt gacgcgcctg tgctgatgtc gtccattgta agttcttcgg ctactctgcc
     1681 gccaagcatt acaattattc tgtccaacat ttcatttttt gatatgtaca ttttatcttc
     1741 atcaggcaca tacatcgtat atccgccggc tctgccac
//
LOCUS       DAWACS010000307         1777 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6623_length_1777_cov_2.144599, whole genome shotgun sequence.
ACCESSION   DAWACS010000307 DAWACS010000000
VERSION     DAWACS010000307.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6623_length_1777_cov_2.144599"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          532 a          334 c          337 g          574 t
ORIGIN      
        1 aaacaaagtt gatggcggca tacggtacgg agctggaatt ttatgagggt gaaaaaatgg
       61 tacacgactg gattttggca acaccgtttt atcttgaggc atttaaagcg attgaaatgg
      121 tcggagaatt ttatgtgaac ggctaataaa aaagcaagct gatttcagct tgcttttttg
      181 atattattct gtaatataaa ttcttgtata gaacggaatt gtatttgcaa tccagttaat
      241 atcgcttggg tgaagacgta cacaaccgtg agaaatattt actccgacac gtccgtcata
      301 ttctgtaccg ttatagtaaa gcaatgttga atgaagtgca taaccgttat agaaacgaag
      361 tacaggacct acatagtagc ttggataatc ccattctgtg cgttcgatat attcaaattg
      421 acctgtgatg gtaggcgtat tccatgcacc taatgcacaa ggaaattctt tttgaagttc
      481 ccaattatat tgtgagcctt gatatactct tactttatat tcgtgctttg aaacccatac
      541 aagataattt gtacggcttg taataccgtt cttgtttaca ggtgttctgt tgcgatattc
      601 tttaaccata ggagattcac caatgataat atcaaggtgg tcgtcgaaat caagaacatc
      661 aagacttgaa ttaaacgatt cggcaagcgt tcttacagga acaaatagag aaccctcgat
      721 ataaaccgtt gcgtgaggtg cgtatgtatc gtttccgaaa atcgttgtat atgcactgtt
      781 tgcattaaaa ataacttggt ctgaaccgac gctgcaaact acgctgtcat atttagggtc
      841 gtatgtaacc tttaatccca ttgcctcacc gattgcgcga acaggcatca ttgcgatacc
      901 gtcaactatt cttgcacgag gtgaaacatc ttgaagaaag ttaccgctta catacataca
      961 aagttcttta tcgtaattcg gattaccgtt cattgcacaa ccgttatttt gtacatattc
     1021 gccgttttcg ttcaaatatc cgtacgtctg aacttcaaga atgccgtcag gctcaatctt
     1081 tttatcgtaa tatttcaatg aacgaagtcc cgaaacaagc tttaccttga atttttcgcc
     1141 catacgatat tgcggaacgt tgaatataag atttagcgaa gaagtatctt gagatacatt
     1201 ttcggtaatt gagccgagta attcatcgga actgctgtaa agttcgatag ttacgtcatt
     1261 aggtattatg tacttatgtg atgttatgtc aatattaaga acaaattgtg ttgataattc
     1321 gttcaccggt attgtcggcg ttgaagtcgg cttaactgtc ggctctgcgg tcggtgttgc
     1381 tgtttctgtc ggctcaactg ttgttcccgg tgaaacggta ggttcttctg ttacttgcgg
     1441 tgtttctgta ggtgcaggtg acgctgtttc ggcgaatgca cctattccgc tcaaaacgat
     1501 tgatacggca agaaacattc ctaaaaattt tttcatattt atctcccctt aaactattat
     1561 aaaataatca tatcacaccg aattatcaaa gtcaataaac aataaaagct aaacataaat
     1621 tcggattaat cactaaaaaa acacatcaaa ttttaactaa aatgatgtgc tttttttact
     1681 tgacttattt ttcacgaata aaaattcttt caatgtccgt acccaaaccg ctttgttcgt
     1741 caatgtcaaa aatacaaccg caaaattgtc cttttcc
//
LOCUS       DAWACS010000308         1770 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6653_length_1770_cov_2.619242, whole genome shotgun sequence.
ACCESSION   DAWACS010000308 DAWACS010000000
VERSION     DAWACS010000308.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6653_length_1770_cov_2.619242"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          521 a          363 c          286 g          600 t
ORIGIN      
        1 acacttaaca atttatgctt gtcaacataa tttgttttaa aaccgtttac ttcttcctcg
       61 tccaagaaat actcaagtgt taggtcaaag ttatctggat tatcaaaacc ttcgtccaaa
      121 aattttactg actttaacgt tttcaaagtc actatataat ttacacactg tactatattg
      181 ttaggtgtaa ttctaaaaaa ttcacttata ttcatagtct tcctccatat taacttacgc
      241 ttctaaatga cggtgctgcc attcccgaaa ctgtcttagt atctgttgtt gacgtgcccg
      301 taccatttgt gcctatccaa gttgattgat ttgtatagca agcctcaaag aaacaataat
      361 ttttcaatgt agccaaactc gccgccgaaa accataactt acagttagat gccatacaat
      421 aagcacagaa aatcactgac gccgatatat acaagtcaca atggttaata tgagatgcac
      481 taaaatagcc ttctgcttga agtatcttaa ttcggcagtt agcaagactg ttaaagtttc
      541 ctgttgtcgt ttctgattga ctaaatcgcc tacattcaat ttgtgtgcca ataatattca
      601 ttgtagagtc gtcctcaata tagctatgat tgctactaac agttgtgcct atatgcttta
      661 taaaaccgcc aaccatacta ccctcacacc tgtaaaaaac accattgcta agtgtgttat
      721 tacttgttgt aggaaatatt atatctgtgt caataatttc ggcagtacaa tctctaaata
      781 tccaccaaca aggatggctg ttatcatagc gttttgcagg caatgttaca gatattctac
      841 tattctttaa cttcacttta caattaaaga atattgaatc tgtgttgtgc attgtgtttg
      901 cataactgat tgaacaatta tcaatttgca gtacgtcata atcgttaaaa gcacctgcat
      961 taggactaat attacttgtg gataaaaaat ttatatccat atttgtaaga tgtaatgttg
     1021 gacttgtacc cacaaggttt gatataagaa aaatataact tgtgttgact ttagtaatac
     1081 ccctaccttg tccctcaatt acaacattct tcttttgtag aagtctgctc gacaaattat
     1141 atgtcccctc taaaaggata atttttcccc cgtctgttaa agagtccatt gctttttgaa
     1201 taactatatc gtcatctgtt cccgaacatt taaagtcaca gtaccttgtc accgtcgatg
     1261 aaccgactgt tataacagat ggcttacctg aaacaaactg cttatcagat gatagtgcta
     1321 cccagtatgt accatcatac acaaattcaa caatttcatt cgtcgaccaa gaattgtatt
     1381 gagttatagc atcatatgaa ccgtctgctc gtaatagctt tacttctttt gctcctgttg
     1441 aatttacatt tagcgtaact ttaccttgtg ttccggcgaa aggataagta aaccttacta
     1501 aaaccctcac tcctgttagt agtttaaaat tagtaatgct caatcttcta tcgcttattg
     1561 atggactgtc ggggcatact gcataaggcg gttgttgcca tatcggagca ccgcttccgt
     1621 tgctgattaa attatatccg gtcgttcccg cacttgtcgg cgcataccac gacttgcttg
     1681 ccgttgccga accgttatag ctcgttgctg agccgttcat tgtcagtgtc aatgaattag
     1741 gattttgcat tgatgtaggc ttgttggata
//
LOCUS       DAWACS010000309         1760 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6707_length_1760_cov_1.979472, whole genome shotgun sequence.
ACCESSION   DAWACS010000309 DAWACS010000000
VERSION     DAWACS010000309.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6707_length_1760_cov_1.979472"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          556 a          275 c          448 g          481 t
ORIGIN      
        1 tgtttacttt gttgattaca gccaagacag tgaaatttac agagtgttgt tccagcctac
       61 atcaaagatg attgaatcaa tcaagagatt taatccgaga aatgtatttg taatggagga
      121 ctaaaatatg aaagcgtgtt ttgcctctga aatgagaggt gtggacaagg cggcaagcga
      181 aatcggcgga ataccgagta ttgtgcttat ggaaaatgcc gccatggctt gtgtggaaga
      241 acaaaaaaag actttgcaaa cctttcggaa aaatcggtca cggtattttg cggaaaaggc
      301 aataacggcg gtgacggttt cgcaatcgca agacatctgt acaatatggg cattgatgtg
      361 gcggtttatt tggtatgcgg aaatgaattt aaaggtgacg cgaaaattaa ttttgatata
      421 atcaagagaa tgaatatcaa cattgatgtc atttctgata tagaggattt gaaatatata
      481 gtgcgttcgt atgacataat tgttgacgca attttcggaa caggcataag cggaactgta
      541 cgtggaataa gttatgacgt tataagtgaa attaatgata acgcaaagta tataatggcg
      601 gttgacgttc cgagcggaat taattccgac agcggcgaaa tttgcggcgt atgtataaag
      661 gctgacaaaa cagtgacctt tgtggcttac aaagtcggta tgcttatgtt ccccgcggct
      721 gattatgtgg ggcaggtaac ggttaagaat atttcaattc cggactatat aattgacgta
      781 cagaatttga agattaacgt aattgacgat aaatttgtgt gttcaaattt ccctaaacgt
      841 gaaaacaatt cgcagaaagg tgattacggt aaggtacttg ttatagcagg ttctgccgga
      901 atgacaggcg cggcatattt gtcatcacaa actgccgtta caacaggcag cggacttgtt
      961 actcttgcag taccgagcag tcttaacggt gccatggagg caaaaacgac agaagttatg
     1021 acagtgccgt taagcgacag aaacgggcgt atttcagcta acgcaattga cgaaatttta
     1081 aagtgtgtcg ataaggctga tacggtgctt ataggtccgg ggttgggaag atgtgacgat
     1141 gtttcggaag ttgttgaaag tgtgcttgaa tattcaaaag ttcccgttat aatcgatgca
     1201 gacggaataa atgccgtagc ggaaaatatg aaggctttaa gcagttgcac ttgccctgtg
     1261 atatttacac cgcatacggt tgaaatgtca cgtttaacgg gacttgagag ggaatatatt
     1321 gaggataaca gacttgttac cgcaaaggag tttgcggagg aaaacggagt cactttgatt
     1381 ttaaaaggac atcatacgat tgtaacaggt caggacggag aacaatatat taatattaca
     1441 ggcaattcag gtcttgcaac aggcggcagc ggtgatgtgc ttgccggaac ggttgcgtca
     1501 cttgtatcaa gaggtataaa cgaaaccgtt gcgtcagcta tggcggtgta catacacggt
     1561 ttggcgggtg atattgccaa agacaagtat ggaatcgagt ccgtaaccgc atctaaagtt
     1621 atggagtgta ttccgtgtgc tttaagacaa atattacaag tagaaaatta aatggctata
     1681 tgctataatt aagaagaact gaattaaatc tctcgcagta atgtggatga ttttctttcg
     1741 gaaagggagt agtataacaa
//
LOCUS       DAWACS010000310         1757 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6720_length_1757_cov_1.595182, whole genome shotgun sequence.
ACCESSION   DAWACS010000310 DAWACS010000000
VERSION     DAWACS010000310.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6720_length_1757_cov_1.595182"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    292..301
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    672..681
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1344..1353
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          552 a          271 c          424 g          480 t
ORIGIN      
        1 atatgacaga gtccaaaaac catcatatgt attcgcactt tatggcatgg cttatgacag
       61 atattatagg aatacagcct gacaatacaa acggttatag gattgatata aaaccatatt
      121 atgtaaaaga attaagttat gttcgcggtt attatagcac tgaatacggc agaattaccg
      181 tggaatggaa acgggaaaat ggagctgtaa atttatgtgt tgaggttcct caaaatgtaa
      241 atgcaactta caacggacaa aaactccgca caggggagaa taaatttatc annnnnnnnn
      301 ngatataagt atgtcattat atgaaaatac cattaagatt tacacgcaat atgattatga
      361 gcttggcgat ataagtaatg aagcaaatat taagctacta aaattgaggc tggattttaa
      421 ggaaaaaata aatcctgaac ctgttgtaat taaattttat gtaaaatccg agaatgtttt
      481 tgcgcaatgg agtccgactg ccgaatacat aaggaatata cgatttgact ggagcaaggc
      541 agaatgtaaa tcaagcgcgg cattcggcgc acctgtgcag tgcttatacg gagctgatgg
      601 tattaacagc atgacttgtg caatagcgga ctttaaaact cccgtctcga taaaatgtgg
      661 ggtgtgcgag gnnnnnnnnn nattgagatg gatatagaat ttttcacctg tatgacggct
      721 aagcgaagct cgtatgaaac tattataagg cttgatatgc ggaatattcg tatttatgac
      781 ataatgcgtg atgtttcaga gtggtggcgg aaatcaggtg atttgccgga tatgagcgtt
      841 ccggacatag caaaacatcc tatgtattcc acatggtaca gttaccatca gaacatagac
      901 acagaagcgg ttgttgccga atgtaaaaaa gcatatgagc tcggtatgcg cgcggtgatt
      961 gtagacgacg gatggcagac ggacaatagc gaacgcggat atgcttattg tggtgattgg
     1021 aagcccgcga taaaacgaat aggagatatg aaagcctttg tagacgcagt acatagtacg
     1081 ggaatgaaat ttatcctgtg gtacagtgtt ccgtttgtgg gtaaatactc cgatgcatgg
     1141 aataaattta aaggcaaatt tcttgacagc gccgataatg attattgcgt gcttgaccca
     1201 aggtatcgtg aggtacgaga ttacctgacg ggtatatacg aaaatgcagt tagagaatgg
     1261 ggaatagacg gatttaagct tgattttata gattccttgc gtatgacgga atcttcaaat
     1321 gaatacaatt cagaaatgga tacnnnnnnn nnntgataga gtttcgtcag aattatatag
     1381 gaccgcttat gcagacttac ggcaatatgc tcagagcaag cgactgtccg tatgatgctt
     1441 tgacaaacag aatgagaact cttgatttga ggatgatgtc agggtgcggt gttgtgcata
     1501 gcgatatgct gatgtgggat atgtcgggga gcgcggagaa tgcggcaagg cagataacaa
     1561 acgtattgtt cagcgtgcct cagatttcgg ttaagcttaa tgatataccg gctgaacata
     1621 tggagatgct taaattttat ttaagcttct gcgatagata ctgtgatgtt cttttagagg
     1681 gatctttaca tgtatatcag cccgaaatgc tgtattcaat ggcatatgcc caaaaaggca
     1741 atcgtgctgt tgcggtt
//
LOCUS       DAWACS010000311         1752 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6745_length_1752_cov_1.933412, whole genome shotgun sequence.
ACCESSION   DAWACS010000311 DAWACS010000000
VERSION     DAWACS010000311.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6745_length_1752_cov_1.933412"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    978..987
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          479 a          352 c          299 g          612 t
ORIGIN      
        1 atatcatcat ttgtaagaag ttttgtaccc gaatttgcaa gtatatagtc ccctgcataa
       61 ttcgttatac tcgtaatttc atatacagta tttccgttat acgtttccga cgtcggtaca
      121 gccgttatcg tgtaaggctt gccgtttatc tgttccgttg tactaccagc tgaaacagtt
      181 gcataatatt taacattatt ggttacaaat gtcattaaat gttttccggc atttgagtca
      241 tatataactt caccgcctgt acttagctgt gaagaaatgt ttgaagatga aactccgctg
      301 ttttgtgcag atgatgtatt tgacgatgag cctgtaccgc ttgaacttga tgtacttgat
      361 gatgaaccgc ttgaacctgt tcctttagat gatgtacttg atgagccgtt tgacgtatta
      421 ctcttagatg ttttgccgct tgacgatgta cttccgctcc ttgaggctga tgtaccgctg
      481 cctgtattac ctgttgcaga ccacgattgg ctgtttgatg atgtactgtt ttcatctctc
      541 ggagatgttt ccattgacaa ctcaggcgtc tgtacaggtt cttcacttac cgcaggtgtt
      601 gcaattacac tcggtgttga cacaactgcc actgacggtg caggtgtttc ctctattttg
      661 tttccgccgt tgacgttaga cacaagataa tacacaccta ttccggcagc agcaactgca
      721 agagcacaaa ttgcaccgat aagcacaata cgattacgtc tttgtttttt tcttttttca
      781 ttacggcgac gttcgatttc agacaactgt tgtttcttat cctccatcat tttctttacc
      841 tgttcacgac gtcttctttc attatcatca tagatttcat caccttctga atttgcttgt
      901 tcttcagaat tatcattttg tttatcaccc aaattttcat tcaactcggc ttgatgttcc
      961 tgatacgcat tttctatnnn nnnnnnnttt tgtttcttct gccgtacttg catcttcatt
     1021 aacttttgta ccgcaaaatt cacattcttg agcatcatcc ggtatctgag ctccacatac
     1081 tttacatagc atatattcta ccctccgatt tcaatatttc atacctattc aattatcatt
     1141 atattatgtt cctcatttca agtcaatatc gtattgttta caataaaaag acattttacg
     1201 tcaaccattt taattttacc caattttccg aattttaaca aaaataagga catcaattta
     1261 gctatttgta cgatatgtta agcatattat gtgtaaagga ggttatgaga tatgtttctt
     1321 ggcggtttat tttttgaatg gatacagact gttttaatac ttgccggata cgtttcgggt
     1381 aatgcgacat tcccaaagcc attaaacaaa agcgaagaaa agaaatatct tgacttgtat
     1441 gcaaaaggtg atgaaagtgc aaaaaatgta cttatcgaac acaatctcag acttgttgcc
     1501 cacattgcaa aaaagtatgc tgacgaaaaa aatcttgagg accttatatc tatcggcaca
     1561 atcggtctta tcaaaggaat aaacacattc aatcccgaaa aaaattcacg tctgtctaca
     1621 tatatttcac gttgtattga gaatgaagtg cttatggtta tgcgttcgtc aaagaaactt
     1681 caaaatgaaa tttccattga tgaaagtatc ggcacagacc gtgagggcaa caatatgact
     1741 ttttcggata ta
//
LOCUS       DAWACS010000312         1746 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6772_length_1746_cov_1.531047, whole genome shotgun sequence.
ACCESSION   DAWACS010000312 DAWACS010000000
VERSION     DAWACS010000312.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6772_length_1746_cov_1.531047"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    559..568
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    938..947
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          611 a          267 c          335 g          513 t
ORIGIN      
        1 ccgtaagagc agacggcgtt tacggcagca atacaagcgg cagaagcgag cctcagccgt
       61 attctcattc ttattcatca gcgataagag cgccggaagt gtgggcgtta gagggtaata
      121 caggcgcata tgtttataaa aagtcaataa gctatgacgg ctacaagtca atgtcctcaa
      181 aagttctgct ttctcaggca aaattgaaat ttgacagcga tacacctaca aacgcatata
      241 tctatatgtc ctctaacagt aatcattcat caggtgcgat tgcttgtgac ataggcttga
      301 taggtgcgcc tgcaaataac ggtggctggt atctgatagc aagccgaaac aataataaca
      361 gcaataccac atcatcagcc ggaatgaaaa ccttttacag ttcccccatt gtgcagtcaa
      421 cattagtaaa cggcgaatac agaccgaagc atgatatata tttatactat acatacggcg
      481 acggaacggt atattgtcag gtgcagaatg taataacggg agtagcgcaa gagggatatg
      541 tggacgatta cagatttann nnnnnnnncg tcattagttc ccgatattta cgatagtacg
      601 ggaacgcaga cggcaggcga tataaaatgc ggcgcatatt taaaaaatgt aatctggagt
      661 gaaaataaaa tttataagca atctttatgg aaaggtacag cgtacagctt cgcaggtaat
      721 aattcatcta ccacaaatta tttactgaca tacgacagag ataatgcaag ctgtaccgct
      781 acaagcgaca gagatactat aaacattttt tatgacgctg catatgaaca ataataacaa
      841 gataagttaa gagaaaggaa tgttttacta tgaaaaaggt aattttaggt ttaataattg
      901 gtatagttat atcgacgggc acaatatatg caaaacannn nnnnnnncat atgtccctat
      961 tagagctatt tcacaggcct ttggaaaacg tgtggattgg aatgacgaaa caaaaaccgt
     1021 tgaaatctgc caaccatttt cctatcaaga agttatgaaa gctcaagatg taattaaaac
     1081 attttttgat gattttgcag aaaataatta tactgaaatg aaaaaagttc ttagcgagcc
     1141 attttctcaa tatgatattt ctaatggtgt atatggtatg aaatcagcca cattatcggg
     1201 aattgagtat atagaagaaa gttcagattg gtctactgat acattgattt ttaattgcac
     1261 tttcgataat gtggagttta ctcctaacgc aagtaataat aatgggaata acaaaggagc
     1321 ttgttatata ttagttcata aagatagaga tacatacact ataagtaatt ttgtttctgg
     1381 actttaaaat actttaagga taaaacctca atatttttct tatacagaat aacttattta
     1441 caaaatcaat ataatatggt acaatatggt acaatatggt tacttttata atgtttttgt
     1501 aataaggagt gaacagcgta cagccacaat gtgtggattg tcaaaaattt atgcgctatt
     1561 ataggattat agggactaat aaaaagtcgg acttgtcaaa aaaatttgtt tgataggttc
     1621 ggctttttta gtttctaatg cgaaaaatga ccgtttaaga ctaaataata tagaaagcgt
     1681 aatattatgt gtatttatga cagatagtat tatgcttttt tctttttata cggcaatgct
     1741 aaatat
//
LOCUS       DAWACS010000313         1742 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6794_length_1742_cov_1.670421, whole genome shotgun sequence.
ACCESSION   DAWACS010000313 DAWACS010000000
VERSION     DAWACS010000313.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6794_length_1742_cov_1.670421"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          661 a          286 c          288 g          507 t
ORIGIN      
        1 tcagtgcttg caactgataa ataaactttg gagagattaa tttgaaaaag atatttttat
       61 tattagtaac cactttattt ttactttcat tatatggatg taacacttct tcatcaagca
      121 ataaccccac tgaacaaaca accgtaacga atacagatga tttatctctt gattcagata
      181 ttgaaacaac agaattaaat catgattatg aaatttacaa taacggcaaa aaggtggata
      241 ctttaaatct ttacggctta tttaaacaaa ttgataacgg cttcatatat tcaaaaatca
      301 ataaagactc tccaaatgaa atggaatact acagatatta tttagataaa aaaaacagtg
      361 taaaacttgg ttcaataaaa aactggtcta tgcaaattca cgataaagct tatgtaaata
      421 atcacctttg ctttacgtca agcacgggcg acattacaga cgaagaaaac agaactcttg
      481 aatttattga tatcgacctt aataaaaaca aaatgacgac tgtattttct gacaaaggcg
      541 gatttccata tgacactgtt acaggagcag acaatcttgt cataatcaca aaagtgctag
      601 acaacggtac ttctttggaa ctgtatgata ccgaaactca aaaattcaag caattaaaat
      661 atttagagtt tgatgataaa aacagtatcg gggaaactat aagaaaagtt tcaattgaca
      721 aaagtacaaa aacaatctct cttttggttt tggattgcat ttcacagaac gaagcttctt
      781 taaaaataga aacttatgac tatgatatga attttctcaa aagctatgat atatccaaca
      841 tttcagatga ttcaaacgaa ttaattcagg gcgtacaggt ttttgatttt aaaaacaatt
      901 atctattcta tcaaaatttc agcataacaa gatgtatagg atatatagac agcgataagc
      961 tgaaaaaatc cgacatatca gaagatgtag atgatacttt ttcaatagta tccgcatctg
     1021 aagatgacag tgatacaaat ttattgtata agagggcgga atcaagttca gaaaataact
     1081 acatatatct ttttgataca accaacaaaa caatgaaaga aacaaaattt aatattgaag
     1141 aaaaagggta cactataggc ggtatatcac ggacaggaaa agataatttg atgatattga
     1201 tgtcccctga taaggcagac aaaaaaagcg accttaattc aagaatatac tttacaaaac
     1261 taagcgactt aaattttcag taaacaggcg gtaaaaatca attaaaatcc attttgcaaa
     1321 ttatctgtca aaatcctgca aaaaatttaa tttatgggca atactgctct ttacttttta
     1381 aggtatttat tgtataatta gcggtaaagt aaaaaggtat atttaatgtt acaaaacacc
     1441 ttttgctcac aataaaaagg ataggaggga caaaacataa tgatcagcgg tgctgaaatt
     1501 atggtaaaat gtttggaagc cgagggcgtt gacatagtct ttggttatcc cggtgcggct
     1561 atctgcccat tttacgataa agtttacgat tcgagcatta cccacattct tgttcgtcag
     1621 gaacagaacg ctgctcatgc ggcaagcggc tatgcaagat gcagtaatcg tcccggtgtt
     1681 tgcgtggcaa cttccggtcc cggtgcaacc aacctcatca caggcattgc aaccgcctat
     1741 ac
//
LOCUS       DAWACS010000314         1741 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6799_length_1741_cov_1.827402, whole genome shotgun sequence.
ACCESSION   DAWACS010000314 DAWACS010000000
VERSION     DAWACS010000314.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6799_length_1741_cov_1.827402"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          595 a          264 c          423 g          459 t
ORIGIN      
        1 aaaagataat tcgtgatgca aaagcatatc ttatggaaat acacagaatg tcggaggaac
       61 aggcacatcg atttatacaa aaaagaagta tggatacagg tgcgaagttt attgatacgg
      121 caagaatgat tttaaatatt taatggagat gaagtaaatg cagacattta agttttcaaa
      181 aatgcatggt gcaggaaacg attacatata tgtaaattgt tttgaggaaa aagtcgagaa
      241 tataaacgaa acggctaaag ccgtaagtga cagacatttc ggtatcggtt cggacggact
      301 tgttttgata tgcccgtctg atgtggctga tttcagaatg gatatgtaca acagtgacgg
      361 ctcacaagct gaaatgtgcg gaaacgcaac gcgttgtgtc ggtaagtatg tgtacgacag
      421 aggtcttaca gacaagacac agattactct tgaaacgttg gcgggtataa aaattcttga
      481 cctaaatgtt aaagacggag aagtgcagac tgtttgtgta aatatgggca gtccggaact
      541 tgttccgaaa aatattccga ttgactcaaa tcttgacaga tttataagtg aagatgttga
      601 agttgacgga aaggtttata aggttacagg cgtgtcaatg ggtaatcctc atgcggttac
      661 atttattgat gatacggaca gtcttgaaat agaaaaaatc ggtcctaagt ttgaaacgca
      721 taaattgttc ccaaagaaaa tcaatacaga atttgcacag atagttgaca gaaacactat
      781 aaaaatgcgt gtttgggaac gcggtgcagg tgaaacactt gcctgcggta caggtgcttg
      841 tgcaacaatg gttgcggcaa atttaaacgg tcttgttgat gatgaggctg atcttgtact
      901 tttgggcggt acacttcata ttaagtggga taaaaatgaa aataaagtat atatgacagg
      961 tccggcaaag tttgtatttg acggaacaat aactatataa gagcttgtcg acaaaatgtc
     1021 gacagctcca aactgataaa tttgaacata gaacagaaag gaacaaataa aaatggcaaa
     1081 ggtaaatgac aattatttaa aactaccggg aagttactta ttctcaacag tcgcaaaaaa
     1141 ggcggctgca tatacagagg caaatccaga caaagaagtt atcaagatga gcataggcga
     1201 cgttacaaag ccgttggctc ctgcggtaat tgaggcaatg cacaaggcag ttgacgaaat
     1261 gggtacggca gagggatttc acggctacgg tcctgagcaa ggttatgatt tcttgagaaa
     1321 caagattgtt gaaaaggatt atgcggcaag aggtattgac gtaaaggctg atgaaatatt
     1381 cgtttcagac ggtgcaaaat cagactgcgg aaatattggt gatattttct ctgttgacaa
     1441 taaagttgca gtgtgtgacc ctgtttatcc ggtatacgtt gatacgaatg ctatggcagg
     1501 cagagcgggt gactttacag acggtaaatg gaataatctt gtatatatgc cttgcaagga
     1561 agaaaacgga tttatgccac aacttccgga agaaaaggtt gatataattt atctttgttt
     1621 cccaaataac ccaatcggtg ttgcggcaac aagagaacag cttaaaccgt gggttgaata
     1681 tgcaaagaag aatgatgcgg ttattcttta cgacagtgcg tatgaggcat ttattacaga
     1741 c
//
LOCUS       DAWACS010000315         1719 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6912_length_1719_cov_1.719952, whole genome shotgun sequence.
ACCESSION   DAWACS010000315 DAWACS010000000
VERSION     DAWACS010000315.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6912_length_1719_cov_1.719952"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    550..559
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          518 a          337 c          308 g          546 t
ORIGIN      
        1 ggtataggcg gtgtgttgct ttgttcgctc ggctgtatca ttccgtcatt tgtcatatgc
       61 tttatactcg catatttcta ctataaatac cgcactgtcg gcggagttca gacggtttta
      121 tccgcccttc gtcctgctgt tgtcgcactt ataacctccg caggcacatc tatactaatg
      181 ctcgcacttt tccaaaccga tatttttact gcatcgttaa aagatattcg ttttgtcgaa
      241 ctcggtatat tccttgtggc gctgttttta ctcagaaaat ttaaaacgaa cgcaattaca
      301 attattcttg gcagtggtgt tgtcggaacg ttgatttata tgctttttgg aatggtataa
      361 tatttcttta aaatttacac taaaatagtg gacttttatt gatttttgta ttataatagt
      421 gattgtcgaa aaacatatta aaacggagga ttattatgat aagacaagtt ataaaaatag
      481 atgaagataa atgtacaggt tgcggtattt gtgccgatgc ctgtcaagaa ggtgcaatag
      541 ggattgttgn nnnnnnnnnc ctgtccgact aatgcgattt cttttgaaac ccgtgaagcc
      601 gccgcatttg atgaagatgc ggtaaagcaa aatatgcaga aagcaaaaga acaagaaaac
      661 cctgcgtttg tctgtccggg cagtatggca aaggctataa agcacaatac cgaaaccagc
      721 acgcctgttt caacgagcca actttcacaa tggccggtac aaatcaagct tgtacctgtc
      781 aacgcaccgt attttgacaa tgccaatctt cttattgcgg cagattgcag tgcatacgct
      841 tacggcaatt ttcacaacaa gtttataaaa aacagaatta cactaatcgg ttgtccgaaa
      901 cttgacgata ttgactacac cgaaaaattg caagcgatta tcgaaagcaa caacattaag
      961 agtgttacga tagttcgtat ggaagttccg tgttgcggcg gtattgagta tgccgcaaaa
     1021 acagcgttgc aaaacagcgg taaattcatt ccgtggcaag tttatacaat cggtacaaac
     1081 ggtgaaattt tagattaatt tattaacata aaaaagagaa tgctgtcgca ttctcttttt
     1141 tatggtttaa catccaatcc tgtactttca attacaataa ccgatttaat tctcttcccg
     1201 tttccataaa ctgttttcgc cgtaacttta caacgattat acataccttc tttatacaag
     1261 aggtcaagca caacactaat cggcttatca cttttcttta aatgcttaca taatattttg
     1321 tattcgtcca tcgaatcgtg atgaacaatc ttattgtcca aaatatattc aagtgcattt
     1381 tgtatgtaag tgccaaacat ataagaatgt ccttcggcaa gttttgtctt tacatcaatg
     1441 gtattgttta taaaataata ctcaagtgca atggcgtcaa taaggtcaag tgccgtttca
     1501 aaactgcgtc ttagcatatt gagtttactc aaaacaccca tttgttcact gctgtccgaa
     1561 acagacgcat aataaattga acaatcttca cggcttgcaa ggtagaaaat tttaatcgac
     1621 agccatataa catcttcacc gaatacattt ctaacctgta cggaaacgcc tttattccca
     1681 gccattttag attcgcgaaa tgcctttatt gccttttct
//
LOCUS       DAWACS010000316         1711 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6960_length_1711_cov_1.269324, whole genome shotgun sequence.
ACCESSION   DAWACS010000316 DAWACS010000000
VERSION     DAWACS010000316.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6960_length_1711_cov_1.269324"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    223..310
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1408..1417
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          323 a          434 c          424 g          432 t
ORIGIN      
        1 atctgttgct gtaccgtgac cgttatcgcc atcaagcgaa gccgtgttgg taatcgttgt
       61 gccagccgca ctttcaagca cctgcacgtc aaaagtgatt tcagccgatt cgcccggttc
      121 gagatcacct acgagcacag acagcgcatt gccgctcaaa tgatacagcg caaattcgtt
      181 gttgatatac acgccgccta ccagcttaac accagtcggg agnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn cgcttgcaga cttctttacg atgggagcag cgttgccctc gtcaatttgt
      361 acgccgccgt ccggcatggg cgtttccttg ccgttatcat cgaggatagc ggtattgaca
      421 atgaacttgc cctgcgaacc atcatctacg attacatcga agctaagaac ggtctgtgcg
      481 cctgctgcga tagcatccgg cgtgaacgta attgttttca tcgtagcatt gtacgaataa
      541 ttgaccgttg ccgaaccatt ctcctgcacg tttcctgcaa aacttacgcc gtcaggcaga
      601 acgtcaatca ctttcatgtt ttcccaatca gcggtagctg ctgcaccatt ggaaaggcga
      661 atggagtagg tgatcgtatc gccaactgct gcggtggtct tgtttgcggt cttattgccc
      721 gcatgaccgt ctgtcttacc atcttcaatg tccacaccgt tatctgtacc gctcttgtcc
      781 ggcgtattgc tgccgctgac gagggcggta ttttcaatgt gcataccgta agcgtcgctg
      841 ttgacggtta catcaaagca aatcgccttg gtctggttcg gcgcgatctc accgagcgat
      901 acggtcaggg tcttgccggt attgtcgtac tgatattctg cggagtagcc gtctacggtt
      961 acattgccgc ggaaggtcag tccgtccgga atggtatcgg tcataaccgc gtcgcggata
     1021 ttcacatccg caccagtcgc attcgctgcg tttacggtgt aggtgatcgt atcgccaacc
     1081 ttggcagtgg ttttgtctgc ggacttggaa acggtcagca gcgcgtcacc gtccgcgatt
     1141 gctacaccat cgtccttgtc ctgcaccggg tctgcattgt cagaggtcag cgttgcaagg
     1201 ttctggatgg tggtgttgta cgcggattca ttcaccgttg cggtaaagcc gatgatctgc
     1261 tttgcgtccg gctcaatcgt gccgacattg acggtcagca ggcgggtatt ctcatcgtag
     1321 gtataatcgt tggtgttctt gccgttcaga gtgacagagc catactcgaa agttagtcca
     1381 gccggaagaa catcggtgat aacgccgnnn nnnnnnngta ataacgccgt tctcaacagg
     1441 aacagttgcg gtatcatcgt tctttacggt cagcgtgtac gcgatcttat cacccacgcg
     1501 ggcagaggac ttgtttgcag tcttgtcgat ctgcggcttt gctttgccgt caccaacggt
     1561 tacgccgttg tccttgccgc tggtgtccgg cgtattgtcg gacttcatca cagcggtgtt
     1621 gtaaatggtc ttgccgtatg cagcggtatt caccaccgca acgaaagtta ccgctgcgct
     1681 tttctcaata ccgagatcac cgaccttgac g
//
LOCUS       DAWACS010000317         1711 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6957_length_1711_cov_1.615338, whole genome shotgun sequence.
ACCESSION   DAWACS010000317 DAWACS010000000
VERSION     DAWACS010000317.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6957_length_1711_cov_1.615338"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          544 a          286 c          317 g          564 t
ORIGIN      
        1 taccgcttcc acaacaaagg cagaaaatgt gcaatccgta ccgacaagga cagcttccac
       61 ttcatcaatc acatcgtttg gaaatcgtat acttttgctt gttgtttggg gaatttgcgg
      121 aattttaaac tttttcattg cgtgaactgc tcctgtatta cattatatat tactattgta
      181 tgcaatttac acaaaaattg cgtattactt ttgtcttgca ttttgtatta cgcaatgtta
      241 taattgaatc aatgaagctt catatctaaa aaatttaata aggaggcaat aagtgattac
      301 tgttattggg tatattctga caactctcgg ctttagcgga cttgcatatt cgttatttac
      361 tgtgaataca aacaaaagat attcgtggac atctccattt tcttcatttg aaaaaacaat
      421 tattgcagtt gctataattt catttattat tctgcttgta ggaattatta tggttatatt
      481 ttcttttgtt aaaaagaaaa atgaggataa gcttaattca cttaaaaatt taggaaataa
      541 caataaatgt ccaaattgcg gtcttaatgt taccgcaggt attaacgaat gtccacgatg
      601 ccaccaaaaa cttaataata ttgacggagg tttttataat ggcaagaaca attattaacc
      661 tgccactgcg aaacagtgtt gatgaatcat acaacactgc tgtaaacatt ttgcaggcaa
      721 acggttttaa agaagtttcc tacaacggcg aaactgtatg gaaaaaaggt acaggacttt
      781 tgactgctat gcaatacata aaaattgaat ttacagatac aacgctcgtt gtatctggtt
      841 gggtacaagc aggtgtcggt agtgttggcg gaaacgaaat ggcacttaac ggtattgttg
      901 ccgctgtacc aaaaaaatct gttatgaagg tcattgaaca gattaaggca agtctgtaaa
      961 cagaggtaac aatatgataa aattaagaaa aatatcaatg gtgttatgta taattacgat
     1021 gttgtttgcg gttatgtcat taagcggttg cagtaacaac agcataaccg gaatatgggc
     1081 atttattgac gacgatgggc aagttagcga aaattcaaaa atgtatttta atgatgacgg
     1141 ttcttgcaac gatattgatt atcatggtcc gacaagtgct gaccctgtgt catacaaggt
     1201 catgagtgat ggaaaacttg tattcacaat ggaatgggac ggcacatcat catttgacct
     1261 tgctcaaagc gaagaggaag ccatgaatga ttatcacaaa tattatattt caggcgacac
     1321 attgattttc aggaaaagca catacaagag agtataaaat tacaccccga ttcgtttgaa
     1381 tcggggtttt gtttattatg aatatgattt tatctgaaca tatactgtgc ggtgtcgagg
     1441 actgactcca ttgtcttaaa ggctttgttt gcctttttct tgagttttgg gttgctgtct
     1501 accatatttt ttccgacaat acccatgcag acgcctgccg tcattcctat tgcaacgcct
     1561 ttcatcacat tcatcattgg acttgtattc ataatcacac ctcccaatct tgcaaattca
     1621 ttgacaaaat gtgtgttttt tcatctttgt cacacggctt tgtcaaaaaa acttgcatta
     1681 ttattgttta cgatttaaaa tgtatttaaa c
//
LOCUS       DAWACS010000318         1710 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6963_length_1710_cov_1.743807, whole genome shotgun sequence.
ACCESSION   DAWACS010000318 DAWACS010000000
VERSION     DAWACS010000318.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6963_length_1710_cov_1.743807"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    460..469
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          470 a          383 c          252 g          595 t
ORIGIN      
        1 gtccttgatt gtgtccgctt accaaaaagt atcccgcaaa gcctgacgca accgccaaaa
       61 atataaccgt tacaaccaca attacaataa tagcgatttt attgtttggt tgatacccct
      121 cagaattaaa ttgttgagat tgttcatttg tttttacaat tttttcaccg gtcattggat
      181 caaagttata gtcatcttta ataatttttt cgccggtcat tggatcaaag ttatagtcat
      241 ctttaataac tttttcaccg gtcattggat caaaattata atcttctctg ttcatatcat
      301 tatcccccta tctgacgtat gcacttgtca catatccgac cataccgtta taataaacct
      361 tatcatatcc gttgtacgag ccgagatatt caactgccgc tccgacagga atttcacaaa
      421 tgtattccgc cggctctgac ggtgtttttc ttaaatacan nnnnnnnnna attttgcact
      481 cctgaaatat gataaatcgg atttccgcct gtgtagacat gaggatctgt tgatgtcaaa
      541 tattccgctt tggcataacc gatttgtccc ttgtatttta ttttgtagaa accgtttgta
      601 acatcctcaa tatatccgac aggttcaccc caagggattg tacagatatt ttccgaattt
      661 tcttccgcat actttcttaa atatattgag ttctgtacat taaaaacata cattgtgaat
      721 ttaaccgata catcggacga gcctgtcgca tgttgcaccg gttcagcagg tttggcattt
      781 ttattgcctg cctgttgaat tgcaatattc gcgtcttccg taagatactt tgtcattaca
      841 aagccttctt gtccgccata cttaattttt gaaaaatcat cattttcctt ttttacaaat
      901 tcaacctgcg taccaagcgg aattgtggta atctgttcac cgttttggtc cggagttttc
      961 ctcaatgtta cgctttcatt cacatttaca atgtacataa ccgttgtcgg attttctgtc
     1021 ggtgccggtg ttgcaagagt tttatcgaca accttgtcac acaacacata tccgtccacg
     1081 ccgtttactc ttatcttata aaaaccgcca tgcgcaagtg ccaaaaagct taccgtttca
     1141 tttgcttttg tctgcataag cactgccgag cctttatcgg gattttcata aacattcgta
     1201 ccgtcatcta aaattgtata tgacatacct tcattaatgc tgtttaaact tacatttccg
     1261 ttgcttgcat tattgaatgt tgacgcatac ttttgatatg caaaaacaac tccgccgaca
     1321 gacagcaatg caactgccgc acatataacg caaacagcga ttaatttgtt ccttgaattc
     1381 attcccctca tcctttcgct taacagattt aagtttttat tacagatata caaaattata
     1441 taataataat taaatatatt atatcatttt cgacaacagt tgtcaatata ttccacaaaa
     1501 taaaaaacgg tttaaatttt tcgtttaaac cgttcttcaa atttatacca cagattccat
     1561 tgtacctttt tcccaacaga atatacgcat tgtttcaccg ctatatcctt tgtattttta
     1621 cctgccattt tgcagtccgc cacctctacc actgcatgcg gcatatttgt ttttctccaa
     1681 agctgcgggt caaaagcgtc ctcctaatac
//
LOCUS       DAWACS010000319         1707 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6971_length_1707_cov_1.883172, whole genome shotgun sequence.
ACCESSION   DAWACS010000319 DAWACS010000000
VERSION     DAWACS010000319.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6971_length_1707_cov_1.883172"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    566..575
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          573 a          234 c          430 g          460 t
ORIGIN      
        1 atatattggt gtcgggtgag ttttttgaat ttccgtcggg aagaaacaaa cttcatatcg
       61 taaattcaaa cagtcaaaat atgacggccg aaatttcata tacaccgtat tttatgtacg
      121 gtgaacattt tgacgacttg gaatggggtg acggaaatgc ttagaatatt accgtataac
      181 agtgttacgt ttgacaaggg tattgtaatc ggtgacgcgt atgatgtgcg tgttacgtat
      241 gaaataaacg gtgaacgcag gcttgatttt tcgcacccga taaatgaaaa gtcggaaatt
      301 ataagtgaaa ataaaatcgt tgtatgcgag ggtcaggcat acagaattat aaaggtgtca
      361 aaaacaatcg gtgaaaagaa ttttattgcg gcggaatgca gtcacgtcta taatgcggac
      421 gcatcgaaca tacatattca aaatattccc gatttaatcg gaaaaactcc gtcttatgtt
      481 ttggggcaga tatttaaaaa tacgaagttt tcgattatga cggacagtga attgacaaaa
      541 gtcggattga aacgagttga ttacannnnn nnnnntttct atggataaga ccaatccgta
      601 tgaggcggtt aaggctctta tcgaaaactg cggtaagggc gagatttatg ccgacaatta
      661 caagattgcc cttgtggagc gtataggcgg tgaaagctgt ttaaggcttg acctttcaaa
      721 gaatatgaaa gatatttcga tagaaaggga tataaccgat atggtgacga agttgtaccc
      781 ttacggaaaa gatgacgcac atatcggaag tgtgaacagc ggaaagcagt atattataag
      841 tgaaaacgcg gatatttacg gtgttcgtga ggggtatcgt gattataccg attatattga
      901 accgagtaaa atattaagac gcgcacggtg ggagtttgac agtgaaaatg aggaaagaat
      961 tgacgtgcct tgtgttaata taacaggcgg atatgccgat atttcaaagc tggcggatta
     1021 tgcggacgag aaaattaata tcggtgatac ggtgactgta atcgactgcg gaaacgagat
     1081 tagagaacgc gtcataaggc ttgaatatta tccttatcaa agtgacgata ccgttatatc
     1141 tgtcggcagg gtaaaaaagg atttgttttt ctatcttgaa caaataggaa ctcttgcaaa
     1201 acgttacaaa aaagtatcga caacaggcgg taaagtcaag gcgaaatcgg taagcggtgt
     1261 tatttcacag tccggaatga aaatagacgg tgaaaacggt acggtttcgt tgttatcgga
     1321 cattattgag gtgtcaacgg acggtgacgt aaaaacgcag attggcaatg tgaacggtca
     1381 gtttgtgttt aatattacgg acaacaacgg caattcggct gtgaatatta cggacaaggg
     1441 caatatgaat tttaagggcg attttgaaac ggaaaaattg agcgtggggg ataatgttat
     1501 tacacaggac agtaacggta tgttgtgtat taacggtaaa aggatattgg tggaaggaga
     1561 ataagaatga aattagattt taattttgac ggaaaaactt tgctgaaaaa atggtggcaa
     1621 caggtgaaat cgaattttca gacggtgcag gatgaatata atacgttaga ggataatttg
     1681 agtgaagaaa taaatcaacg tgtgaat
//
LOCUS       DAWACS010000320         1707 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6975_length_1707_cov_1.771186, whole genome shotgun sequence.
ACCESSION   DAWACS010000320 DAWACS010000000
VERSION     DAWACS010000320.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6975_length_1707_cov_1.771186"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1240..1249
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          518 a          295 c          405 g          479 t
ORIGIN      
        1 ccttacaacg gaagaaggac ttataggaaa attattcgca ccgttcggta taaaaatagc
       61 acatacaaga atcggaattg tagtggcgct tatatttatc ggcataccgt tcgttgtaag
      121 agcggtacag ccggtgcttg aaaaacttga tccgtcatat gaagaagcgg cgggagcgct
      181 tggtgcaaac agaggaacga tatttcgtaa agtcatttta cccgaaataa ttcctcctgc
      241 acttacaggc tttggcttgg cactcggaag atgtatagga gaatacggca gtgtaatttt
      301 tatagcaggc aacaagcctt atgaaacgga aataacaccg cttataatta tgtcggaatt
      361 acaggacttt gactataaca gtgcaacggc aatagcactt gtgatgcttg cattctcatt
      421 tgcaatattg tttattgtaa atcttattca ggcgagaaac acaaaaatat taaaaggagg
      481 aagatagtat gcagaaaaat gaaggcatta aggttgttaa atggatattg attgccgtca
      541 gcgtattatt cgcatttgta ttcttaattc ttccgcttta tacggttata tccgttgcac
      601 ttcgtaacgg tgtgggaaaa tattttcagt acataaccga taaagatacg ataagcgcgg
      661 taaagcttac cgcaaaagca actttgtcgg cacttgttgt aaacacattc ttcggactgt
      721 ttgcggcatg gttcttgaca atgttccggt ttaaaggcaa aaaggttttg acaactctta
      781 ttgatattcc ggtaacggtt tcacctgtta tagcaggttt gatattcata ttggtattcg
      841 gcagacaaag cgtgctttat ccttatttac agcaagccgg tataaaagta gtgtttgccg
      901 taccgggaat tatattggca acgatatttg taacgtttcc gtttatatca agagaaatta
      961 taccgatact tgaggaacag ggcacagagg aagaagcggc ggcacttatg ggtgcaagcg
     1021 gatttacgat tttcagaaaa gtaacattcc ctcatataaa atgggcattg ctttacggag
     1081 tggtactctg tacggcaaga gcgatgggtg aattcggtgc ggtatcagtc ctttcgggac
     1141 atttgagagg taaaacgaat acgcttccgt tgcaggttga aatacttttc ggcgacaata
     1201 aatatgtacc ggcatttgcg gtagcgtcaa tacttgtgan nnnnnnnnnc aactattctg
     1261 cgaatgattg cgggtcttga aactccctac agcggagata ttataatcga cggtgtgaga
     1321 gtaaacgacc ttgcggcaag caagagaggg attggctttg tatttcagaa ttacgcactg
     1381 tttcgatata tgacggtgta tgataatatc gcattcggtc ttagagttca gaaagccgat
     1441 aaaaagaaga tagacgagag agtaagagaa cttattaaac ttataggtct tgagggtttg
     1501 gaaaaacgtt atccgagtca gctttcgggc ggtcaaagac agagagtggc attcgcaaga
     1561 gcgcttgcac ctaatccaga gcttttactt cttgacgaac cgtttgcggc gattgacgca
     1621 aaagtaagaa aagagcttag aagctggctt agagaaatga ttgaaaagct tggagttaca
     1681 agtatatttg taactcacga ccaagac
//
LOCUS       DAWACS010000321         1707 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6974_length_1707_cov_1.836562, whole genome shotgun sequence.
ACCESSION   DAWACS010000321 DAWACS010000000
VERSION     DAWACS010000321.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6974_length_1707_cov_1.836562"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          458 a          389 c          279 g          581 t
ORIGIN      
        1 ctctctgcca ttaacgtatc ctccccgatt atttgttatc tttattttct tcttttttat
       61 cttccgactt attttcagcc tcacgtcttg gctgttgagc agaagcttta cggaatgtag
      121 cacccataag ttttcttata tcataaatct gctctgaatt tgtttccgaa acagctacgc
      181 ttacttgcat tgtaacgtta ggcgtctgta cattatgata atgcacaagt tcttcaaggt
      241 actgcttaaa gtcctctgca cgctgaacgg tacactcttc aaacataccg ataaactggt
      301 cacttccgtt atatccgaca aatccgtaac ttgacgcggc tctctttagc accttaccga
      361 atactttcaa aagtgcatcg cctgcctcac gtcctccctt gtcattgaca tacttcaagt
      421 tatcaagctt aataagcaag aatacaaatg agtctttaag cttatcagct gcatactttt
      481 caatctcaat atcacacatt gctctgttcg gaagtcctgt cttacggtca gtgtacataa
      541 tataatcaag gaagtctttt gaacggccga gtataatagc accgatacaa ccgccgaaga
      601 agaatatgat aatcgcaaga acgatataca ttcttacatt aatcttctca cttgtcgaaa
      661 tacttgaaag tgttgaaacg ttatatgcac cgatatattg gttgtattca tcaacagttg
      721 tcgaaacact tgaatacata tcaccaagct tttgtgaaag ttcttcaagg ctctgttcga
      781 tttcgtcctt attgctcata tcatcagatt gagcgccgtc agtaaatacg gcaagcagct
      841 ttttcttatg ctcaaggtca acttcaagtt tttccttttc tgtttcaatg tccacgtact
      901 cattaataag agtatcgtat gttgtttcac tctgaacgtg accgtcctct tgattccatt
      961 catcatacac attcttcaag atgtagtcac ttgcgtcatt gccctcgtca ctttgtgtac
     1021 cgtagtgata ttcaacgccc tctttattct tatcactgta ctttgtaata aggtcattta
     1081 agtcgttgat tttctcctcc atattctgca aatcaagttc atatgtactt atatcactct
     1141 gatacttctt aataagagtt tccttatcgc ctgtaacctc ttgttccaat ataagcgaat
     1201 aaatctgcgg aatatcgtaa tctatatatg acttataaat ttcatacaag tcactgaatg
     1261 aatatcccgt ttccgcactt ctgtaattcg gctgagtttc ctttttagca gcaagatagt
     1321 cggaaatatt aagcgcccac gcgtcaagga tttccacacg ttcaatataa tcatactgac
     1381 cgtccgttcc gcgcacgtta ttattaggca atgtttgttg gttgatatat ttttcaccgt
     1441 atgttgcgaa gtaattcttg attatcgaat caagcacggt tcttgcaaaa tcttggctgt
     1501 actcactgcc gacagagaac gacacaacat agtctgtagg gaaataagta tattcttcac
     1561 cggcttccaa aagagcctgt tttttaactt cttcgtccgg agggataatt tccgtaacct
     1621 tacattttga acggattgcg tccgcaccga tattcaagtt aaggtcctct aatacgttgg
     1681 ttattaccgc cgccgaataa atttccg
//
LOCUS       DAWACS010000322         1706 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_6980_length_1706_cov_1.854028, whole genome shotgun sequence.
ACCESSION   DAWACS010000322 DAWACS010000000
VERSION     DAWACS010000322.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6980_length_1706_cov_1.854028"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          610 a          252 c          367 g          477 t
ORIGIN      
        1 gggtagtctt gacaaaaaca agggcgcttt gtttgtaaac gcaggtattg catttaaaaa
       61 tttccaaaag gcatatgatg aaatacttgt gcagcttgaa aatgttaaaa acggcgaaat
      121 atcacagctt gaatatgagt caagcattaa tgcaattata aattcgcttg aatcatacaa
      181 ggacgaccaa gatatgttgc agtcattcta tttgggacag aaaatagtcg gaactgatta
      241 tgatatagac acgcttaaag aaaagataag caagataacg atggaggacg ccgcaagagt
      301 gtttaaaaac gttaaacttg aaacagtgta tttcttaacg ggaaaggagg atgcgtaatg
      361 gaattgcagt acagaaaaaa tgacaaaaca ggtgaggaac tgtattacac aacacattct
      421 tcaggactcg gaatatatat aattcctaaa aaagattaca gcaaaacgta tgcaatattc
      481 ggtacaagat acggctctgt cgattcaaag tttgtcgtac cgggtgagaa tgagctaacg
      541 gaagtgcctg acggtatagc acattatctt gaacataaaa tgttcgacca gccggacggc
      601 agtaatgtat ttgacaaatt ttcaaagtgc ggcggcaatg caaatgcgtt tacatcattt
      661 aatataacgg cgtatctgtt ctcggcaacg tctaatatcg aagaaaatat cgaaacgctt
      721 atggactatg ttcaaagtcc gtattttact gaggaaagcg tacaaaaaga acagggtatt
      781 atcggacagg aaataagaat gtatgacgac agcggaagct ggaagttgtt ctttaatttt
      841 ctgaactgcc tgtacaaaga acaccctgtt aaaaaagaaa ttgcaggtac ggttgaaagt
      901 ataagccata taacgcctga atatttgtat aagtgctacg atacatttta taacttgtca
      961 aatatgtcga tagttgttgt cggaaatgtt gaccctgtta agatttctaa ggttattgaa
     1021 aacggtgtaa agaagaatga gccgtttgaa gagaagatta aaaaggtata tccgacagag
     1081 cctgatgaag tggcaaaaca ttacgcagaa caaagtttga gcgttgcaat gccgttgttt
     1141 atgacaggtt ttaaagatac aaataacggt ttcggcggtg atgagctttt aaagaaaagt
     1201 atagaaacga atattatttt aaaaatgctt ttcggaagaa gttcaaagtt ttataagagc
     1261 ctttacgaaa aaggacttat aaacaacaga ttttcacttg aatacacaat gcagcccgat
     1321 tatgcgtatt catcaataga cggtgaaagc agtgacccta aggcagtgta tgaggcggtt
     1381 ataaacgaag ttgaagaaac aagaaagaac ggtctttcaa aagaagattt tgagagaatt
     1441 aaaaaggttg tttggggcga atatatccgt tcacagaatg atgtagaaga ttatgcagtg
     1501 acatttttac agatgttgtt tatggatatt gattatttca atttctatga tgtgtataag
     1561 acaataactt ttgaagatgt tcaaaaacgt tttgaagaac atttcgtcaa agaacattcg
     1621 gcattgtcgg taatcaatcc tctgctcgat tattattttg actgccaatc tcccgataag
     1681 gtttttgagc ttatcaaaaa taccgt
//
LOCUS       DAWACS010000323         1702 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_7005_length_1702_cov_1.588342, whole genome shotgun sequence.
ACCESSION   DAWACS010000323 DAWACS010000000
VERSION     DAWACS010000323.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7005_length_1702_cov_1.588342"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    649..658
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          561 a          265 c          368 g          498 t
ORIGIN      
        1 cgattatgat gagtgttgat actgtggaag tgagcataac gagaaaatgg cacagggcat
       61 atattgcact cggttcaaat atgggtgaca gcaaaggtca ccttgacggt gctgtcaaag
      121 aacttgacgc ggataagtgt tgcagagtga aaaaagtgtc ggagtttatc gtgacagagc
      181 ctgtcggcgg tgttgaacag gatgattttt taaacggctg tcttgaactt gaaacacttt
      241 acacaccgta tgaattgctt gattttttgc ataaaatcga acaggcacat aacagagaac
      301 gcattgttta ttgggggccg agaacacttg atttggatat tattctttat gacgatgttg
      361 ttatgtacga ggacgattta atcatacctc atatcgaaat ggaaaacaga gaatttgttt
      421 taaaaccgct ttgtgagata gcgccttata taaaaaatcc cgtaaatgga aaatcaataa
      481 aacaactttt aagtgaaatt caaaaataaa taattttgta caaaatattt ttatactttt
      541 tatgcaaaat aatacgatat gtcacttgaa aaatatcgct acttatgata tatttgtaat
      601 gtaaatgtaa aataaggtcg tagacggacg aaaaccgtca aagagagann nnnnnnnnat
      661 ttaatacata tccagtaaga aggagaatta caatgacaaa caactttata agtaacgaaa
      721 caccgatagt cggcaagctt agtatcataa gtatgaaagg ctgtgaaggc ataacggcta
      781 agattgatga atatcttaga gcttttagag gttatgagga tacagaaact tatgttactc
      841 atgtaacttg tccgagattt ggtacaggcg aaggaaaatg cgttatcgat gaaactgtaa
      901 gaggtcatga cgtgtatatc atatgcgatg tgttcaatta cggtgttacc tataaaatgt
      961 acggagttga aaatcatatg agtcctgatg accactatca ggatttgaaa cgtgttattg
     1021 ctgcaatggg cggtaaggca agacgtgtta ctgtcgtaat gcctatgctt tacgagggca
     1081 gacagcacag aagaaacggt cgtgaatcac ttgatgcggc acttatgctg caagagcttg
     1141 ttcatatggg cgtgtcaaat atcattacat ttgacgcaca cgatccgaga atttcaaatg
     1201 caattccact tgcaggtttt gatgatgttc aagcgacata tcagatgata aaggcacttg
     1261 tcagagctgt tcctgatata tcactaaaca aaaaagatac tattgtaatc tctcctgatg
     1321 aaggcggtat gtcaagatgt atgtactatt catcagtgct aaagcttgac cttggtatgt
     1381 tctataaaag acgtgattac tctgtaatcg taaacggcaa aaaccctatt gaggctcacg
     1441 aatatttggg acgtgacgta aacggtaagg acgttattat cgttgacgat atgatttcat
     1501 cgggtgaatc tgtaattgac gttgcaaata accttaaaaa gcagggtgca aaggcaattg
     1561 ttttaacacc gcattattat tcggattcca taagttatga tgattttgtg gcaaggcgta
     1621 acaatgctta taatttgctg aaatcatctt tgccactcga aagtcctaaa cttattttat
     1681 cggcagaggt gtatatttcc aa
//
LOCUS       DAWACS010000324         1692 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_7050_length_1692_cov_2.355528, whole genome shotgun sequence.
ACCESSION   DAWACS010000324 DAWACS010000000
VERSION     DAWACS010000324.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7050_length_1692_cov_2.355528"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1308..1317
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          587 a          278 c          405 g          412 t
ORIGIN      
        1 atgggcattt tggccgccac tgtttttcct ataacactta taatcacttt aataataatg
       61 aatttgcaca aaaaaagtct gtacaacacg aaatatttgt gatataattc caaatataaa
      121 taaattatga aggaagtgtt atgaagatga agaagaacaa aattcgtgtg atttcagcgg
      181 tggtttctgc gataatgctt gcaagcagtg caagtgcgtt tgcaaaattt gacgcatata
      241 atgatcccga tggttttacg cttggtcatt atgaggtgga agtaaatcaa gaacaacgtg
      301 ccgatacaga aaaattgtat gagcaaagac caaaaaatga aagacagttt gaaaatcttt
      361 caagaggttt ggtggcagta ccggaagaaa acggaacgct tgtaagctgg cgttttttag
      421 gtacggattc aaacagcttg acatacaatc tttattgtag cggtgaaaag ctgaatgaca
      481 agccgataac tacgacaaac tttttccata ccggagcgag tacaaatgca gagtatactt
      541 taaaagaagt agagaacggt gaagaaacag gagtagagta tacgactacg gcatgggata
      601 aaaattatat tggctttaaa gtaacggagc gtgaaggata caacattgat gacggtgcgg
      661 ttgcagacct tgacggtgac ggagaatacg aaattttgtt aagacgagtg ccgagtatgg
      721 acgttaacac aagaacatca tatcctgtta tcgaggcata caaaacagac ggtacacata
      781 tgtggacaat cgatataggt cctaacgaaa ttaatgaagt agatataaac ttccttgcat
      841 atgatatgga cggcgacgga aaagcagaag ttataatgcg ttcgttcgag ggtacaacag
      901 acggaaaagg caatacaaca ggcgatacga acggtgacgg aataaccgat tattcaaagt
      961 cggagagcaa tttggcaata tttaaggaca gacaatacat agtttcaaca cctgaatttc
     1021 tttcgatata tgacggcgaa acaggtgagg aaacagacag aacagaccta aaaccgtcaa
     1081 aagaaccgct ttcggactgg tcatacagat acagtgatac aggacgtctt acaaagagag
     1141 caagccacta tcttttcggc ttggcatatc ttgacggtgt aacaccgagt gttgttatgg
     1201 tgcgtggtgc gtgggataac gtaagagcgg cagcatggca tattgaggac ggaaagttta
     1261 aagaagattg ggtacacaat accgaaaaca aagatgatgt aaacagtnnn nnnnnnngct
     1321 atatggcgtg ggcttgtcac gaaacatcac agcttatggc gaatatcgag tatcacgatg
     1381 caagaacagg tgaagttcaa tggggttaca gcaaaaataa agatacaggt agaagtcgtt
     1441 cggcggatat agaccctaca cacaaaggct ttgaagtatg gggctcaact gcaacaattc
     1501 ctgccaatat cagcggtgaa aatatagctg atacatggaa tggttttaag ttcagaaaaa
     1561 ttgacggtac tgttgacagc gacacaacta ttccgatgaa tttcaaggtt tattgggacg
     1621 gtgatttact tagcgaattg cttgacggta caaccgtatc aaaatataac tgggaggcca
     1681 agtctgttga cg
//
LOCUS       DAWACS010000325         1688 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_7068_length_1688_cov_2.309859, whole genome shotgun sequence.
ACCESSION   DAWACS010000325 DAWACS010000000
VERSION     DAWACS010000325.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7068_length_1688_cov_2.309859"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    467..476
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          523 a          255 c          373 g          527 t
ORIGIN      
        1 cctattaata tacatcggaa aggaaaagaa aaatggcatt tgtaagtgaa acgcatttga
       61 cagtaagata cgcagaaacc gatatgatgg gaattgtcca tcattcgaga tattatcctt
      121 ggtttgaagt tgcgagaacg gatttcatca aaaaaatcgg tatgagctat accgaaatgg
      181 agaaaatggg gataatgtta ccgcttaccg aaacagctgc aaagtattat tacggtttaa
      241 aatatgagga cgaagtgctt gtaacttgta aaatgacaaa gcttaccgtt gcaagatgtg
      301 aattcactta cgaagtctat aaacttccgg aaaagaagct tatgactgtc ggaaagaccg
      361 gtcacggctt tgtaaaccgt gaatttgtac cgataaatct taaaaagcta tatcctgaaa
      421 tatggaacaa aatgcacgac cttttggagg attaaaatat gaaaatnnnn nnnnnngcac
      481 aagggaatag gtgtgtgcaa cgtaaaaggc tattcaaccg attccgttgc acagattacc
      541 gtttctatgg cattgtcatt ggcaagtcat ttgaatgaat ttaataacta tgtcaaaagc
      601 ggaaagtata tgataagcgg tgtgcaaaac cacttgaaac cgtattttaa tgagcttaaa
      661 ggtaagactt ggggtgtaat cggacttggt aatatcggtc agagaacggc tgatattgct
      721 tatgcactcg gtatgaaaat tgtgtcaaat tcacgcagat accatgaggg gtatgagtgg
      781 cttgaacttg acgaattgtg taaagttgcg gatattatat ccgttcatgt accgcttaca
      841 agtcaaacta aaggtatgat agacgaaaaa cgcatttcat taatgaaaga cggtgttata
      901 tttattaatg tttcacgcgg tgctgtggca gatgaaaacg cacttgttac ggcggttgaa
      961 aacggcaaaa taggcggact gggtgttgat gtgtattcgg ttgagccgat tacgaatgac
     1021 agcccgtaca atcgaatttt agaccgcagt aatgttatat taactccgca tatggcatgg
     1081 ggtgcgtatg aagcgagagt cagatgtatg aatgagattg ttaagaatat agaaacgttt
     1141 atgatgggcg gtaaacgcaa cagagtagat attgatatat agaatagagt ttggcgacga
     1201 tttcaaaaaa ttaagtaata aaaaggggac actaaatttt tagtgtcccc ttatgctaat
     1261 ttgatttaat ttcagttaat ttaatatcat cagtcgcttt gattttttct ttttcggaaa
     1321 gtgtaaatga atacgaatta ttataagcaa cggccaaatc ttcgcttaca cgagctttac
     1381 cgcttgatga ataaattaca agagttcccg tacctgtggc gatgtatcgt ccggcaggga
     1441 tttctgacgg gcaagtatag ctttggtctt tctttaatgt atatgttttg cctgtctttt
     1501 cagttgtttg taatgacgag ttttgatttt gcagttcctc aatttgtgag tcgagttcag
     1561 atgacttttg atttaattcg gtaatgtcat tttttaattt gtcgttcttt ttctttgcct
     1621 ccataaagta attgtttacg gtggtttttg tactcaattc cgaatttagg tcatttatct
     1681 gactttcc
//
LOCUS       DAWACS010000326         1688 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_7072_length_1688_cov_1.452541, whole genome shotgun sequence.
ACCESSION   DAWACS010000326 DAWACS010000000
VERSION     DAWACS010000326.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7072_length_1688_cov_1.452541"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    416..430
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    913..922
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1144..1153
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          510 a          286 c          308 g          549 t
ORIGIN      
        1 ttgctgattt tcagatgtta catttgtgtt ttctgtttga ttttccattg taatcttcct
       61 cctactacat atagtattat ttttatgata ttaaaatata taattaaata gtattcttat
      121 atgtattcaa tataagaata taggattatt ttagcataga atatataatt gtgtcaagag
      181 gaggaatcgc tttgaaaaaa tttgaaccta aaaaacctga aaaagaagtt atttcactta
      241 gattggatag tagaatgtta gaagaaatca acagttgggc tattgaaatt gatataagcc
      301 gaaatgaatt tataaatcaa tgtatagatt ttgcgttgaa aaacatagat actgatttgg
      361 ttgaaagtgt ggaaagaaaa cataatacaa acggcagagg cggacgcagt cagatnnnnn
      421 nnnnnnnnnn aagaagatga agaaaaccaa aaaaaagatg agggctgacg tcctcatctt
      481 ttcttgggtt ttgtatttaa tttagtattc tgttttacat agtatatgct tctcgaagca
      541 tatgaatttc ttttttataa ccgtcaagct catttggtgt ttcaagcaaa atcggaatac
      601 cctgtaattt agggtgattg ataatcttaa ccatagtatc aaaaccgata ctgccttcgc
      661 cgattttttc gtgtctgtct ttatgacttt caaaagggtt tttactgtca ttcatatgaa
      721 ttgcttttaa tctgtcaaga ccgataattc tgtcaaattc atcaagaaca ccgtcaaggt
      781 cattaacaat atcatatcca ccgtcataaa tatggcaagt atccatacac acgcccattt
      841 tattatcaag tttaacaccg tcaagaattt ctttcaattc ttcaaaactg cgacctattt
      901 ctgtaccttt acnnnnnnnn nntcgttgtc atatcgggtg taagtacagt attaagctgc
      961 tttacgataa aatcaattcc gatttcaacg ccttgtccga cgtgactgcc cgggtggaag
     1021 ttgtacagct ggtttggtgt atactccata cgtcttaaat cgtcagccat tgtatttaag
     1081 gcaaattcgc gagtttcttc tttattggaa cacgcattaa gcgtatacgg tgcgtgagcg
     1141 agannnnnnn nnnttttacg tcttgttcgt caatagcttt cgctttacct ccgcgtggat
     1201 tacgcgtgaa aaattgaaaa gtatttgcgt caatgctctt tgcctcttta cccatattca
     1261 tatagccttt tgcggctgac aagtgacaac ctatatttaa cattctgatt tcctcctata
     1321 atatacttgc attcataaca ttattatagt ataattgaag aataaaatca acaataaaaa
     1381 ttaggagaaa tatttaatga ttacagtaac aaacgtaagt ctgaatttca gcggacaaaa
     1441 tctgttttca gacgttaatt taaaattcac accgggcaac tgctacggag taatcggtgc
     1501 aaacggtgcc ggaaaatcta ctttcctgaa aacgatttcc ggggagctgg accctacgac
     1561 gggttccatc atgctggctc cgggagagcg tctttccgtt ttgagccagg accactttaa
     1621 gtgggatgct tttacggtga tggacacggt gatgatggga cacacggtgt tgtgggatat
     1681 catgaagc
//
LOCUS       DAWACS010000327         1687 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_7074_length_1687_cov_1.882966, whole genome shotgun sequence.
ACCESSION   DAWACS010000327 DAWACS010000000
VERSION     DAWACS010000327.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7074_length_1687_cov_1.882966"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    388..397
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          546 a          276 c          369 g          486 t
ORIGIN      
        1 atgcacaaca tagaaagcgg aaaccaatcg cggtatgcta ttgttacggt agacttaaac
       61 ggattaaaga atataaacga cagccgtgga catcaaatgg gtgatattta tattcaaaac
      121 ggtgccgacg cgatacgaaa agcgatggaa ggtactgatt ttatcggtta cagagtcggc
      181 gacgatgaat tttctgttgt attaaaagac tgcgatattg atatggttaa gaattttgcg
      241 gacagaatcg caagaatggc agacagtatt aatcgcggag gaattaagac ttctatgtca
      301 atcggttatg ccaagttcaa cgcggaaaaa gacagaaact tctctatgac aatggaacgt
      361 gccgacgcaa tgatgtacga aaacaagnnn nnnnnnnaaa taaaataacc tctgacaaat
      421 catacttgtt cagaggttat tttttgtgcg attttacttt actttttctg aaaataatta
      481 tataatataa ttatctgtat ttaaggagaa aaaatatatg gttggtttta atttaataaa
      541 cataataatc aatgtgccta taacgcttat agcgttgacg ggacacgaat ttgcacacgg
      601 ttgggtatcg acaaaacttg gtgacccgac acccgaacgt gagggaagat tgactttaaa
      661 tccgcttgca caccttgacc ctgtcggtac gttgttgatg attttgacag gcttcggttg
      721 ggcaaaacct gtcggtgtaa atccgtcata ttataaagat acaaaaaagg gtatggcact
      781 taccgcggct gcaggaccgc ttgccaattt gattatggct tttgcggcat tgcttatata
      841 tgctatactt cacgttttat gtataaagct cggtttcggt acaagcgttc taaacggtgt
      901 tgcaactttt gcacagcttt ttgcgataag aaatctatgc tttatggtgt ttaacataat
      961 tccgatacca ccgcttgacg gtgcaaaagt acttggaatg tttcttacga acaaagcata
     1021 ttacactatg ctgagatacg aaagatattc tatgctttta attatggtgc tttcgcttac
     1081 cggcgttctg aacggcataa tcggaacagg tgtgaatttc ttctttaacg gaattatcaa
     1141 cctgttaaac agtgcgatta acttgatatt atagggtgaa aaatggataa gatattatat
     1201 aagcttgata cgtttgaagg tccgcttgat ttgcttttga cgcttataca gaaaaacaag
     1261 gtcagtatat acgacatacc gattgttgag ataacggcgc agtatcttga ggcgattgac
     1321 ggaattgaag aggctaatct tgataataca agcgaatttc ttgttatggc gtccaatctt
     1381 ttgtatataa aatcgaaaat gttacttcct aaaaatgagg aagaagagga cgaggaagat
     1441 ccgagagaag aacttgcgcg taggttggct gaatatcagc agtttaaaga ggcgtcaaag
     1501 gagcttagga agagtgagtt tacaacaaaa gaaatggtgt ttcgtgagcc tgaaaaaatt
     1561 aaatttccta tgcccgaata cgatataatg cacgatacaa aagaactgat tgatgcgttt
     1621 aacaagatat ttcaacgcag acttcgcaag gcgaaaccag aaaaacgtgc gttttcaaaa
     1681 attgtcg
//
LOCUS       DAWACS010000328         1686 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_7082_length_1686_cov_2.302269, whole genome shotgun sequence.
ACCESSION   DAWACS010000328 DAWACS010000000
VERSION     DAWACS010000328.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7082_length_1686_cov_2.302269"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          433 a          381 c          291 g          581 t
ORIGIN      
        1 aaacgtattt tttattatgg tgtttaaatg ttaatgtgtg ttgcataata ttcctcctaa
       61 ttcattgcat acaaaaagca cgctatatgc gtgcttgaca tacatttttt attgtgttat
      121 aatttagata taagaggaac ggtaaacagc cgtttctaat acattagttt atatttagtg
      181 tagaaaatat tttctaccca aataaccgtc ctattgcgtt agggcggtta tttctttaat
      241 atccatacaa taagcaaaat caatacaagt tgtattgtgg tttcactcat aatatttcct
      301 ttccgaaaca gagccgccac cgctccccat atatcaaggc ttttcagcct atttttattc
      361 tacactatac ctcatataat gtcaaattac gtttatttta cagtgctttc ttcactggat
      421 agtagttcat atccttaaac cagttttctt caagttctgt ctttgtgaca ccttccggca
      481 aatcgctttc gtcaaagtat gcgtaatagt tgttgtcaaa atcacgttgt acggctgtgt
      541 atgtagcctt tgcagtttgc ttttccggtg caccacttga cgctttagtc ttaccgccta
      601 cgtttgacgc aaagctgtac gaacccttgt aatatcttac ataacggtat gagccgtcgg
      661 atttcataat tctccacgca acaccgaaat aaacggtttt tgtatcgttg ccgacctcta
      721 ctacaccgtc tttttgtgtc agtccacgcc acattgaatc aacttccggt ggaatatcgg
      781 catttgtgat gtcgtgacct aatttttcaa tgtagtttga tgtttcatac gcaccgttat
      841 cggcatcaaa aacatcactg ccgcctgcgt ctgtcggtgc aatttcgaca gtacctctta
      901 aattatacgg gtcaccatat gttgcgccct ctgatgtgtc tgttttaact gcgaaaaatg
      961 tgtacttgtc cacacctatt gtaggtagtg gttttctttt ctctgtattt gccataaatc
     1021 aatcattcct ttctactact ttcgtaaatc tcattgtcct atgttttatg cttttatcat
     1081 cgggatttgg tacgtccatt gtcatttcgt gataatattc attatcagtc aacaatttat
     1141 ataccctctc cgacaattca aaacacgttt gcggataatc ggcgtaaata tcaatctgaa
     1201 cagtcgtatc attcgtaaca accgtattgt catatgacat tgagcctttg tccgttagcg
     1261 tgtaatatgc tattgcaggc aatttattaa aattatcggg ataagcaaaa catacactta
     1321 caccgtctat ttgctttaaa atatcccgta attccaaacc aatatcaaac accgtacccc
     1381 tccttgaatt ttgcgattat ctcgctgatg ttatttttca gtgcaggtac gaggaacggc
     1441 tgtggtgctt gccccgacgt tgtgtaaaat cgaccgccac tgtaatacgt ccagtgtctt
     1501 tttgacgtat gcgaaacaga tttgtcgccc tttgagcctg tgccgaattc gacataaata
     1561 ccgtaatcgg cagtcggacc gattgcaaca ctgtcaccgt ccacttggct tacgatactg
     1621 ccttttaatc gccctgttgc aacaggacag tttgccactg cgtgcgctct tacgacttca
     1681 cccgcc
//
LOCUS       DAWACS010000329         1686 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_7085_length_1686_cov_1.977928, whole genome shotgun sequence.
ACCESSION   DAWACS010000329 DAWACS010000000
VERSION     DAWACS010000329.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7085_length_1686_cov_1.977928"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    867..876
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1406..1415
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          451 a          378 c          295 g          542 t
ORIGIN      
        1 atgcccggaa caagcaagtc catagccgca accgtctttt ggtcatcgtt aagtttcata
       61 tagaaagcct tgatttcctt cggataatct gttacaaaca acggacgctt aaatacctgt
      121 tctgtaaggt atctttcgtg ttctgtctgt aaatcgcagc cccattccac cttattgtca
      181 aacttaacct taccggcttt tgaatcttcg ataagtatat ttacagcctc tgtatatgtt
      241 acgtgagcaa actcactgtt tacaacgtgg tttagacggt caagaagttc cttgtcgata
      301 aattgattga agaactgcat ttcttcggga gcattttcaa gacaatagtt aatgatatac
      361 tttagcatat cctctgcaag ttccatatcg tcttttaggt ctgcgaatgc gatttcaggc
      421 tcaatcatcc aaaattcagc cgcatgacgt gttgtatttg aattttcggc tctgaatgta
      481 ggaccgaata cacaaatcct ctctcctgga agaattggtg aattgcatac gcaatcattg
      541 aacgcactct gaacactgcc gagaatgtat ttgtacgcgg tcttaaatga gcgactgtac
      601 gcaaatattc aaagctgtgt cttttcttct gtaacggata atccggtgta gattcaccgt
      661 cgattacaac gttttcagcc tttaattcaa acggctgttt tgcgtccggt gtaagtacaa
      721 gtgtacctgt tgccgtaatt gcagctgcaa tgtttagctt tgaaagttcc gcaaagttat
      781 caagtttatc cgactcaatt actatctgca aattcttaaa aaatgtaccg tcatttaatt
      841 cgataaaacc gaaattcttt gaaattnnnn nnnnnntagt agctctgtgc caccgcttcg
      901 cattttaggc acctcccctt aaggagaggc ttattatagt agctttattc ctttttcttc
      961 acaagtctta cgcatatcgt ccggccatac agatgcctgt acttcgccga tatgagcctt
     1021 accaagcata tacatacaca atcttgactg accgataccg ccgccgattg tataaggaag
     1081 ttccttatta agaagtttct tatggaaatc aagttcaagt ctgtcctcac agcctgcaat
     1141 cttaatttga gaaagaagtg aatcttcgtc aactctgata cccatgcttg aaagttcgta
     1201 agccatatca agaacaggat aataaacaag aatatcaccg ttaagctccc aatcgtcata
     1261 atccggtgct ctgccgtcgt gtctttgacc cgacttcaat tctttaccga tttgcattag
     1321 gaatactgct ttcttttcgc gaagaagttt attttctctt tctttcggtt caagatcagg
     1381 gtacatatcc tcaagttctt gcgttnnnnn nnnnnaacgt aatttcctca gggaaattat
     1441 tttcaatacc gctgaacttt tggcatactc tgctttgagt cgacttcata gcggtatata
     1501 tacgttctac aaaactcttt aaagtttcaa cagttctgtc tgacttattt ataacagcct
     1561 cccagtccca ttggtcaacg tacatagagt gaagattgtc cacttcctca tcacgtctta
     1621 tggcattcat atctgtatac aagccctcac ccggattaaa accgtatctg ccgagagtca
     1681 ttctct
//
LOCUS       DAWACS010000330         1677 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_7127_length_1677_cov_1.313810, whole genome shotgun sequence.
ACCESSION   DAWACS010000330 DAWACS010000000
VERSION     DAWACS010000330.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7127_length_1677_cov_1.313810"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1020..1029
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          632 a          277 c          370 g          388 t
ORIGIN      
        1 gtctgccacc ggcaataagg caacggctaa tgcaactatt cctataagta aggcagataa
       61 tccgcagaaa acattaacaa tagaatacga cacgatgttt gaaagtgctg atgatgcgat
      121 tggtgcatca agaggtatgt atgctaaaac aaaggaagga aatgaactat ggcttacata
      181 tttcaccgca tcgtcactca gaacagctat tactaatacg ggaggaaact ggtgctatga
      241 acaagcaatg tctataaaga ataaccaatg gcaccatata aaacttgaac ttcatccaaa
      301 cacaggaata ttttcaatat ggctagatgg aacaatgctt caagataatg tgagttttgt
      361 aaaagaagga agttcatttg atacatgtaa aggagccttt gacactttaa aggaaggaat
      421 aacagacttg cgattctatc atacagcttc aaataatatt gaaaatgcga cgtatataga
      481 taacgttaaa gtaacagaag tatcatattc ggaggaggaa attataccac cggcaaaaat
      541 acaggaagca actccacaga taagcattga ttatataaat gaaacactta ccggctttga
      601 gtcacaggaa ccgtatacga taaaggtggg tgaaggtaat gctaaagata taacgcttgg
      661 cgagggcgtg acgacaatat cgcttgatga tgaaaaaatt ggttatgccg gcaaattatt
      721 gtctattgaa attgtaaaaa aagcacgcaa taccgaaaca tataccgaca gtgacgtgca
      781 acagttgaca gttaaagcac gtcctaaggc acctacaacc gttcaaggtg taaatgctac
      841 agaaataggc ggcaaaggta aactcacagg tatgaatggc atgcagtaca aactcaagag
      901 aacagatgaa tggagtagta cgcagttggt tgatacagtt gaggttgatg caggcgaata
      961 caatgtccgc aaagctgcaa cagatacaga ctttgcaagt gaggagacaa caattacagn
     1021 nnnnnnnnng aacatataca attaatggtt tagatgttac attaacagat aacaagttat
     1081 cattagctaa ttatataaca aatgaacaga taacgctttc gatagtaaag aagggtaata
     1141 atgtgacaac ggttgcgagt gaagcacaga ctctaatagt taaggcacgt cctgcagcac
     1201 cgacaaaatc ggaaatcata gtaacacaac cgagtgtgat tggcggcaaa ggtactattg
     1261 caggtatcgc agacactatg gaatatagta cgaataatgg tattaattgg actacgggcg
     1321 atggagatga tataggtgat attgagcctg gaaccacata taaaatccgt tataaagctg
     1381 taagtgcaga cgaggaagcc gaaaggcagt ttaagagtgc agaatattcg gttacaatta
     1441 ttgcgtatga tgcaatgccg gaaacgcagc ctacaatctc gattaactat gtaaacgaaa
     1501 agctaacagg ctttacggaa ggctgtgact atataataaa aatagatgat ggcgtagcga
     1561 cggataaaga taatgtaaca gaggatatcg acattgacaa cacatatttc ggtcataccc
     1621 taaaaatcgt aaaaaaaggt gatggcataa aaacaagcaa cagtgaagcg tttgaac
//
LOCUS       DAWACS010000331         1654 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_7243_length_1654_cov_3.125078, whole genome shotgun sequence.
ACCESSION   DAWACS010000331 DAWACS010000000
VERSION     DAWACS010000331.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7243_length_1654_cov_3.125078"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    567..576
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          520 a          286 c          360 g          478 t
ORIGIN      
        1 acttcaccct ctgcaccgat aatatcgcct atatcaagct ttttgaaacg agcgtattcg
       61 tcctcgccca aaatatcctt tttaacaaaa agctgaattt gtccgtctat atcagaaacg
      121 tgaacaaaac cgactttacc catacctctc tttgacataa tacgtcctgc gattgaaaca
      181 gtctgaccgt caaattgaga tacttgtgcc tttatgattt gcacttcgtc actgacgcgt
      241 ttctttaatt cgcggtcttc ctctgtatag ttttctttaa gttgagcaga tgtgtgtgtt
      301 ctctcaaatt ttgtgatttt aaatgggtca cgtccgtcat tttgaagttc tgtaagctta
      361 tctctgcgga cttgcaaaag tgccgataat tgctcttgtg tttgctctgt gttttccatc
      421 tattgaaaac cctccttaaa tctaattctg aatatactat ataattatat actattttta
      481 ttgaatttac aatgactttt tgagaaaaaa gtcatatttt aagcagaaaa ctgcgtataa
      541 aattatattt tccattgaca taagggnnnn nnnnnntgag aattatataa aatacagttt
      601 agaatattac ggaacggaga tttttaaagt gaaaaatcct acagtaaaaa agatatttgc
      661 gtgcgtggct gtgcttaccg tgttgacagg ttcggcacaa ggtgcgtaca aagttgaaat
      721 agatgacgat acatatgtat cggacgaagt atattttgcg cctgtgtcaa cttgggacga
      781 agtcaaggca caacgcataa atttaaagca aggcaaggtt ttgttttacg cgggcaaaga
      841 aaatgagccg tataaaattg cggcggaggt tatgccgctg aatacgacaa acaagaaaat
      901 tatatataaa tccgaagata taacgattgc gacagttgac gaaaacggtg ttgttacacc
      961 gactgacaaa ataggtgata cattcattga tataaggtgc ggaaacgcat tgtcaaagtt
     1021 gaaagtaagt gttgttaagg gagtagaggg cgttgcaatg tcgcaaagtg aaatgacatt
     1081 gtatgcagat aagcctatta ccgcaaaact tacggctatg gtttcgccga cagacgcgac
     1141 tatacaaaag gtcagatggt acagtgacga cgagtcgatt gcaactgtgg acagtgaggg
     1201 acttgtatcg ccttgcggtg tcggaacaac cgacatttac gcaaaaacgg aggacggcga
     1261 ctatacggca aaatgcacgg ttaccgttac aacatgggaa aaacgtaaag aggatatacc
     1321 tgttgtgtac accgactatg atatgacggt tgatgaaatg gttgaagaac aaatgaccgc
     1381 agaaccgact gtatttacaa acggtgtgtt cccggcgagt gaggaaaatg ttgaacagta
     1441 tgtaaatccg gaaaatcttg tatctggata tgaaaaatat cagtttatgg atttgagtgt
     1501 gtcaaacaat gtcgattccg ctacacttga cacttattta aagggtaaag gagttttgga
     1561 cggtcacggc agtgaattca agaaagcggc tgatgataac aatgtaagtg aagtatatct
     1621 tgtaattcat tcatgccttg aaacaggtaa cggt
//
LOCUS       DAWACS010000332         1648 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_7293_length_1648_cov_1.479598, whole genome shotgun sequence.
ACCESSION   DAWACS010000332 DAWACS010000000
VERSION     DAWACS010000332.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7293_length_1648_cov_1.479598"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    959..968
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          406 a          400 c          271 g          561 t
ORIGIN      
        1 agacaacatc agcatttctt tcaagtccgg tcataaacgc agcttcggcg attgcgctct
       61 ctaaattatt ctttttttcc gcagctcctg taagcgtagt atgtgccgcg tattcaccgg
      121 caaatacctt tgtgtttctg tcatacttat cataacgcgt gtcatttgcc aagaaccatt
      181 ctggtgacat ataataatgc tcgtctacag cataagtaaa actgtcattt ttagagctgt
      241 tttctcttat ccacttccaa gcctcatcaa acgatgttcc gcttgcagac ggtccggatg
      301 ttgaaatgag cttaatatca ggatatacct tatgtatttc ctgttcaaac gcttcgtagc
      361 ggtcatacca ctgattacca tttatatacc attgctcatt gcctattcct atcatggaaa
      421 gattgaacgg ttcttcatgt cccatatcgc atcttacttt tccccatgta gtcgatgagt
      481 ccccgttagc aaattctata aggtccagtg cgtcctgaat gtactgataa aattctgatg
      541 tatatgtttt tccatcgctt tcaaataccg gcaccgtctc tttactctga tattcacacg
      601 ccataccgac atttacaaca ggcacagcat cacagtccaa atactcgcat aacttaaaat
      661 attcgtaaaa acctataccg tatgtttgat tataatgttt aaaaccgttg tcaagtccgc
      721 tgttcgtatg acacgaccaa cggctccagt tctgttttct ttcttctaca ggtcctacag
      781 tatctttcca attatacctg tttgcgagat tatagccttc aattatgcag ccgccgggaa
      841 aacggaggaa tcccggattt aatgctttta atttctctgc caaatctttt ctgaatactc
      901 catcaacagc atctgcggga atacatgaaa tcatatcaaa gtccgcgctg ccctctccnn
      961 nnnnnnnncg gtcttatcag ggactatcgt tgcctcgtac tttgtccata tgtcggtcag
     1021 attatcagca actgttgttt gagccgccag agtgccgccg ctgtagaccg atgccttgat
     1081 tctctctgcg taagtccctt ttttcgcaaa cagagacact ttgtacggct tacccgcttc
     1141 catgtatata ccgtcatatg cctgattttt aaatcctttt tgtatgcctg aagctgtaaa
     1201 ctcaatataa ttcttattat tttcgttcaa accttctgtt gattttgtga gcatagatgc
     1261 tccgcttccg tttgacggat atacgctcca gccataaagt ccgtcataac ttacagatcc
     1321 ctttccattg ctcttttgag cttcaaatga acagttttct atcatttcag catataatcc
     1381 gccatctgct gcgtaattta tatcctcaaa gaacagaccg tacatatttt gttgaatatc
     1441 tacgcctttt ttctggctta tctccagctg ataatcataa tattcctctc cgagtacata
     1501 tacgtcatag cttcttgtaa gcgtgtgcgt atcaaacata attgttgcgg taagcgtaac
     1561 ctttttcttg cctttttcag gtctttttac gctgcctgtg tttgatatta cgtcggtatt
     1621 tgaacttttc catacaatac tatatccc
//
LOCUS       DAWACS010000333         1639 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_7328_length_1639_cov_2.007576, whole genome shotgun sequence.
ACCESSION   DAWACS010000333 DAWACS010000000
VERSION     DAWACS010000333.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7328_length_1639_cov_2.007576"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    536..545
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          538 a          264 c          393 g          434 t
ORIGIN      
        1 tttagcaaag aagttaacag tataatatat gaggcggaca tttttgtccg ccttttgtta
       61 gataagggta aaaaacaaac ggttacgttt gatataaaag agaatggggt ataacactta
      121 ttaaaggagt agaatagatt tatgagaaga acaaaaatta tttgtacaat gggtccggcg
      181 accgacaacg aagatgtgct tagggatttg atgcttaacg gaatggatgt tgcacgtctt
      241 aatttttcac acggaagtca tgaagaagca ttagaaagaa tcaacagaat taaaaaggtc
      301 agagatgaac ttaacattcc tgttgcaatt ttgcttgaca caaaaggccc cgaagttcgt
      361 ataaaggact ttaaggaagg caaggttgaa cttaaagaag gtcaaaagtt cactttgtgc
      421 acagatgacg ttgtgggtga cgaaaatcaa gtttctatca cttatgcaaa tcttccgaat
      481 gacgttaagg tgggaagtaa aattcttatt gacgacggtc ttattgagat ggaagnnnnn
      541 nnnnntcgta agaactgccg atgatataag agaaattaaa actcttttga gagataacgg
      601 cggaagagat ataagaataa tcgcaaagat tgaaaatgcc gagggcgttg ataatattga
      661 tgatattatt cgcgaaactc acggtataat ggttgcgaga ggtgatatgg gcgttgaaat
      721 tgatatgcat gacttgcctg tgcttcaaaa gagccttatc aagaaaacat acagagcagg
      781 taaggttgtt attacggcaa cacaaatgct tgattcaatg ataagaaacc caagaccgac
      841 aagagcggag gcaactgacg ttgcaaatgc aatttatgac ggtacaagtg cgattatgct
      901 ttcgggtgaa acggctatcg gaaaatatcc ggttgaaacg gtcaagacaa tggcaacaat
      961 cgcggaaaga acggaaaatg atattgacta tgtaaaacgt cttgacagaa tgaatttcga
     1021 ttcgcgtatg gacgttacaa acgctatcag ccatgctact tgtacaacgg cacatgattt
     1081 gcatgcggcg gctataattg cgttgacata ttcgggcggt actgcaatgc agctttcaaa
     1141 gttcagacct acttgtccga ttattgcacc gacattgagt gtaaaagcaa gacgtcagct
     1201 taatctttcg tggggcgtta tcccgattat gagtgaaaca agaaagaata ccgacgaact
     1261 ttttgaccat gcggttgaat gtgctcaaaa gacaggtctt atcaaagacg gcgaccttgt
     1321 tgttataaca ggcggtgcac ctatgggtgt tgcgggtacg actaatatta tgaaagttca
     1381 ccttgtcggt catatacttg taagcggccg cggacttgat tcgattaatg caacggctaa
     1441 tgtatgtgtt gctacaagct acgaagaact taaaaagagt ttctgtgacg gcgatattgt
     1501 tgtaacaaac aacgtcacaa agaatatgat acctattttg aaaaaatcag caggtattat
     1561 ttctgaagaa gtgggcgagg cgaataatgc aagcgtgctt gcagttgcac ttgatattcc
     1621 tgtaattgtt gcggcggca
//
LOCUS       DAWACS010000334         1630 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_7379_length_1630_cov_1.758095, whole genome shotgun sequence.
ACCESSION   DAWACS010000334 DAWACS010000000
VERSION     DAWACS010000334.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7379_length_1630_cov_1.758095"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1342..1419
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          549 a          277 c          284 g          442 t
ORIGIN      
        1 gattgttaat cacttatcaa ttccaaataa agccaaacaa cctcttatat tgattaacaa
       61 tcttcctctt aaatataaga agaaacagaa tcaaatcacg gctgtcacat ttatgcacga
      121 tggaaatgaa cttctttgat tcgtggcggc atattcaaaa aaattttcag gagattataa
      181 aaatgaaaaa aaatctaatc gcaataattg cactaacagc agcacttgct gcaagtacat
      241 ctgcttttgc agacacagta caaatttcag aagaagaact taataagcct gtttcgacat
      301 atgcaccgga tgctatgccg acaccggaaa ttcaaggcga cattatgctt ttgaacggtg
      361 aagaaacaga cgtagcagca cctgttgaaa ctgcaagtta tatttctgta gacgtaacag
      421 ttgtaaaaac agacatcgac gttgacggaa ttatcaaaac aacaacagac gttaataaca
      481 aggacgatca aaacaatact gtaaatctaa agattacaga tgatacactt gtatatgaca
      541 atttgggtaa caaaaaagct ctaagtgatt tgactgatgg ttcaaaaatt actgtattta
      601 caggctcata tgaaccaact ccacttattc ttcctgttca gtacacagca aatgtaatta
      661 tcatcaacgg cgaccaagaa ggtaatgtaa atatagatac atatcttgct gatgaagaag
      721 gctatacaaa tgccgcaaat actttgaaaa ttgcaacagc tgacgataca aagattgttg
      781 ataaggatga aaaagaatat aagggtgata taaataaaaa tgatcttatt gtattctacg
      841 atgtatcaac aaagagtatc cctgctcaaa caacacctac aaaggttgta gttcttggtg
      901 aaaacgaaat tgctctaaag cagattgaag ctgcaaaaaa tgctactccg gctcctacag
      961 cttcacctaa agtaacagaa gctccgcaag taagctatgc aggtcttgta aatgttgtaa
     1021 tcggtgataa gaatgtttca gatgtatatg caaaggataa tacaacaatg gttcctctaa
     1081 gagaagttgc agaagccgca ggtttcacag taacttggga tgcagaaaac agagcagtta
     1141 ttcttaatga cggtgtttac agtctaaaga ttggcgaaaa cagctatgtt aagggtaaga
     1201 tgatgccact aacactaagt gccgcacctg aaattgttaa tgatctaaca tatgttcctg
     1261 ctgaattttt cgcagaagta acagaatcag ctacagttga cggtacatca cttgttgtaa
     1321 cagctaaata atttttgaaa annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ttgtattatc aattgttttt
     1441 aaaactaaat gtcatttgtg cgtttacttc gtttgttata ctcattaacg gaccgacttg
     1501 tacagttata gggtttgcag tatcttgcaa actgtatgca cattctaatg tgatagttcc
     1561 tcccggttgt acttccgcta atgacggttg tgcgtccatt acatcgggca tcattgtagc
     1621 tacattacat
//
LOCUS       DAWACS010000335         1628 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_7391_length_1628_cov_2.205340, whole genome shotgun sequence.
ACCESSION   DAWACS010000335 DAWACS010000000
VERSION     DAWACS010000335.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7391_length_1628_cov_2.205340"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          533 a          269 c          392 g          434 t
ORIGIN      
        1 gttgtacgct aatagaaacg aacggaaaaa atattcttgt tgactgcgga atggaacaag
       61 gtgctgatat atttgaaaat caagaaatcc ccgtaaatcc aaatgatatt gaatgtgtat
      121 tgcttactca tgcacatatt gaccattcgg gtaatttgcc gttactttac aaaaacggtt
      181 ttcgcggtaa aatttacgcg acgaatgaaa catccgattt atgcgaaata atgttgccgg
      241 acagtgcgca tattcaagaa actgaggcga aatggcgaaa cagaaaagca aagcgtgccg
      301 gcagggaaga atatgttccg atttatgata tggacgatac tatcggtgtg cttcagcaat
      361 tccgtccgtg taattatgat gagaaaataa gaattttgga aaatgtcgaa ataagatttc
      421 acgatatagg tcacttgctc ggctcgtctt gtatagaaat ttggataaca gaaagcggta
      481 ttacaaagaa aacagttttc agcggtgacg tgggaaatac aaatcaaccg attataaaag
      541 acccgactcc gatttatgat acggacgata ccgattatct tgttatagag tccacctatg
      601 gaaacagatt tcatacggaa gtacctgatt atataacatt gcttgcgggg gaatttcaga
      661 gaacatttga caggggcgga aatgttgtta taccgtcatt tgctgtaggc agaacgcaag
      721 agcttttata ctatatacgt cagattaaag aacaaaatct tgtaaaaggt tacggtgatt
      781 tttctgttta cgtggacagt cctttggcga atgaggctac cgcaatattt ttacagtgtg
      841 acgtaaaatg tctggacgaa gaagcaagag cgttggtgga cagcggtata aatccgctta
      901 cattcagcgg attaaaactt gccgtttcaa cggatgagtc tgttgcaatt aattttgacg
      961 aaaagccgaa agttataata tcgtcaagcg gtatgtgtga ggcgggaaga atacggcatc
     1021 acttaaaaca caatttgtgg cgaagagaat gtttgatttt gtttgtcggc tatcaggcag
     1081 agggaacgct cggcagaatg ttgtgtgacg gtattaagaa tgtaaaattg tttggtgagg
     1141 atatagaagt aaatgccgaa attaaaatgc ttgacggaat aagcggacat gcagacaaga
     1201 acggccttat aaagtggctt aaaacattta agaaaaagcc tgaaattgtt tttgttaatc
     1261 atggcgagga aaagtcttgc gatgaattta ccgattgtat cagaaatgaa tacggctata
     1321 attctgttgc accgtacagc ggtacttgtt acgatttgct gacaggtgaa gtggttgtac
     1381 agacgtacgg catacgcgtg cagaaaaaga aaactacaaa acgtgctgcg actgtattcg
     1441 gcagacttat tgccgcggcg gaaagattgc ttgcagttgc aagaagttgt gagggaatgg
     1501 ccaataaaga tttggcaaag ttcgcagacc agattgatat gttgtctgaa aagtggaaaa
     1561 gatagataac caccccacag tcgactgcgt cgacaactcc cctcaatggg agccatacaa
     1621 aaaacctt
//
LOCUS       DAWACS010000336         1622 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_7431_length_1622_cov_1.601149, whole genome shotgun sequence.
ACCESSION   DAWACS010000336 DAWACS010000000
VERSION     DAWACS010000336.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7431_length_1622_cov_1.601149"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    708..717
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1319..1354
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          409 a          367 c          267 g          533 t
ORIGIN      
        1 cactcatttc caaaatacgt tttgaacgcg gtgtaagcgg aagttcggtc tgctgtggga
       61 gagaaactcc gataccgata tattcgttta ttttatcttt aaccttttcg gcggttacgc
      121 cgtttttctc aagcacttta gcagccactc cgctaccctc tcgaataagt cccataagaa
      181 gatgttcact gccaatatag ttatgaccca tttccgcagc cgcatcgtga gcctcgctaa
      241 tcgcaattct tgctttttct gtgaaatttg agaataacat aataatcaac tccttttctt
      301 ctatgtgtta tttgttattt agatattgtt tcttattaat tctgcacgtt ttttatcacg
      361 ttcattcggt gataattcct taccgtcaca cacaagcgcc ggctctgaaa gcaccataca
      421 ttcaagcggt gcaattttgc tttcctttgg aattataccc aaactttgtc caagcattac
      481 atcggacaaa agtgatttcg cctctgatga cgacacactt cgtgcatatt taagtgtacc
      541 gtatgagcgg aacaatctgt ccgcaatgac gtcataattt ttttctttaa gcatatttct
      601 tgcttttgtt tccatatcga caatctgatt aaccacatta gtcaattttt cgattacctg
      661 actttcggaa agtcccaaag taattctgtt tgaaatctga tacaaatnnn nnnnnnncct
      721 ttgtaccctc accgtaaaga cctcttactt caatgccaag actgcctgcc gaagaaataa
      781 ctctgttgat attctcggtc attgtaagtg caggcaagtg catcattacc gacgctctca
      841 tacctgtacc tgtattagta gggcaagctg tcaagtaacc gaaatcatta tcaaaagcat
      901 atgtgatatt ttcttcgatt acatcatcaa ccttgcttgc aagtgagtaa gcctcgtcaa
      961 gtgcaaagcc gtcttttatg atttgcagtc tgatatggtc ctcctccata agcataatgc
     1021 tcattgtccg gtctttgctt atcataaccg acttaccttt gccctcgcac ataacaggac
     1081 ttataagatg ttcttcggca agctcctgtt ttctgataag cggtgtatta tcaaggtcaa
     1141 taaagtcaaa atcctttgcc aatgtcgaat taccgcccaa aacagcgttt ttaatttttt
     1201 ctgttacttc ctttgtgtcc tttagggcat tcgggaacgg tgttttatca acatttctcg
     1261 caagacgtat tcttgttgaa acaactatgt ccgaattatt atttttatac caaatcatnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgccgc cgaaagttct tgctttagtg
     1381 tttcgtactt tctctttaat ttaagttctt cgcctgactt tgacggtatc ttaccgacat
     1441 gaacggtact cggatgaatt tgccgtaatg tttcggttac aggacctctg aacactttgt
     1501 aacattcact gcacccaagc ttgccatctc gtctgaaatc ggaataagta cgtccgcaaa
     1561 cgggacattt cttttcctct ttataagttg tcggctgcat aaaccaatca aacccatcaa
     1621 ac
//
LOCUS       DAWACS010000337         1619 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_7444_length_1619_cov_2.197570, whole genome shotgun sequence.
ACCESSION   DAWACS010000337 DAWACS010000000
VERSION     DAWACS010000337.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7444_length_1619_cov_2.197570"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          440 a          354 c          283 g          542 t
ORIGIN      
        1 gctgtaggtg tgtaaatttt gaagcttgcc aaatctacag gatatgtacc ttggaaacag
       61 aaatatttct gtgtttctgt atatgtagaa ctcatagctt cagctgttgt ttcgcctaca
      121 agagtacctg cgtcattata taccttaacc cagtaagttc ctacgctttc atctgcagaa
      181 acaattactc tgtaccaatc ttcactgttg acacctggaa ttgactgagt gccaaccgtc
      241 aaagcaccac ttccgtatga tactgtccag ttaggattat tagttgcatt attcggtgta
      301 ttaccgtagt gaccgaatgc cattgcaccg cctgtaaatc taaccttcat atcaacgata
      361 tattgtgacg cttgttctgc aagttgatat acaccgacag ttgaaccgcc gccaccgttt
      421 gatgcaaatt gaagatactt tgtattatct tcatccttct taagtgttag agttcttaca
      481 ccgtttgaac cgtatattga ccagccgtta agaacaagat cctttgaatt gattgtattt
      541 gatgtagctg tgtatcctac aagagaatcg tcatatgaac tcatgttttc aggataaccg
      601 gcatccggag ctatattagt tgaaccgctc gcaccgatta cggcaaacgg agtagatatt
      661 tcaaatgatt catcaagata tgttaccttt gcagtaacct taccgaacca gtttgaaaca
      721 ccgtttctta cattgaagta agcagtggta tttccgttag ggctttcacc gtcagtaccc
      781 tcacctgtac cttctgcctt tgtaagtgta atatagccgt cctcgttatc aagacctgta
      841 actgtccatt ctacattaag accttccttg tttgttaggt cgccgccata tatacctgtt
      901 gccttaattg caataggctt atgtacaggc tcgccttcat taggctggtt aattgtagta
      961 ttaagttcag aagcaaattc aacttttgat agagtttctt ctgccttttg accaaactca
     1021 tcatacttat ctgttgtacg aacaacaatg ttatccaact tcataacact ctgataacgt
     1081 cctgacaaga ataagatacc cgtaatctta tcattaacgc cgtttgtagg aacaatcaac
     1141 ttgtctacaa ccttttcacc gctcttatct gtgatattga cagttgccaa gccttcgttc
     1201 ttgtcaacca atattgtata gtggtaccaa gctgcgtctg taagagttac ggttgccgcg
     1261 tcaacgtcat taatcttaca gttattagaa ttacctgcca tatttatctt gaagatataa
     1321 tttgaagtca tacctgtgtt tgcagtcggc atagttcctg atgctacaac aaattgtaca
     1381 tcatctttgc ttgacttagt taatgcaagg tcaaattcta ccgcatactt atcttgagaa
     1441 gacaaatcaa catcaaatgc cgatgttgca cttcttgtat tcttatctgc gtcagtgtac
     1501 tgtgcatatt tagtattatc tgtacccaaa gtaataccgt ctgcatagtt tgctgatgcc
     1561 caaatattct ttaatgcaga tgtatctgct acatcttcat agtcctgagt atatatctc
//
LOCUS       DAWACS010000338         1615 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_7463_length_1615_cov_2.394872, whole genome shotgun sequence.
ACCESSION   DAWACS010000338 DAWACS010000000
VERSION     DAWACS010000338.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7463_length_1615_cov_2.394872"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    657..666
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          418 a          373 c          313 g          501 t
ORIGIN      
        1 aaactgcata tgcattccct tttggtcaaa gaatgctctt atgtatgacg caaagttctg
       61 aagtcctgca tttccgctca atgccgacgg gtggaacttc tgattgtaaa gtgtaccgtt
      121 tgatgctctg ccatgttcaa gttttgcaac tgagtttgcc gcagccgtag gaccttttgt
      181 atcagcgccc tgtcttggac ctacaccgtc cgcaatcggt gtattggcaa gtctgccgtc
      241 aggtgtcgca cctgtctgct caccaagagg aacgttagcc gatacaggat acaatcctgc
      301 ctggaacata cctccgcgag gatttttatg tttttcaagt tcttttgcgt atatattagc
      361 cacatcacgg gctaacatgt caatttcttc aatgtcgttt ccgtacttcg gaagttcatt
      421 tatcatatca agtatttctt cataacgctt tctctttgtc ggatcctgtt cacctgtaag
      481 gaacattctg caaatatcgg caacttggct ttcattaacg ctctttcctg ctttagcaag
      541 atttataaca acttcttgag tcgcattttt agccgcctta ggacttaaac cttgtccgta
      601 attatggata agtgcgtctt taagttctgc aattgtgact ttcttttcat caaatannnn
      661 nnnnnngcaa taccgaagcc ttgcgggcct gtgaagttat aatgagcgcc gccctcttgt
      721 acagtcttac cctctttaac acaatcttca accatacatg acaggaacgg tagaggaact
      781 ctttccgaat gtgcggtatc tatcgcatta atagcattaa ccataagacc aatcatataa
      841 gtttcctgtg ctttatatgc gttgtagaat tcttcaaatg tcgtcatatc ttcaacatcg
      901 cctgtcttga tactgatttg agcaccctta tcaacaccgt ttgagaacac aagttcaagt
      961 ggacgacaca tattaaagaa tgccgcgtca tgccaaccgt ctgtcttaaa cggtttctgc
     1021 ggttcaacac aaccgataat gccgtattcc cttgcatctg caagagtaac acctctgctc
     1081 ataagtgcag gaatgatgac ttcatcgttg tagtacgcag gcaagcctac acccgttctt
     1141 gtaagctctg cacatttaat caaaaattca tttggcgtgc cgttccatat tcttaccgag
     1201 aatgacggag caggtaattg agtgtgcatt gacgcttgaa tacataggaa tgacaagtca
     1261 tttgttgcgt caagaccatc tgcgttttgt ccgcccgccg ttaaattttg gaacaagctg
     1321 tatcctgcaa agccgtatga cgatgcctca tcccttgctt tattaaggtc gttaaactta
     1381 acccaaatac agtcaagtat ttcctgtgca aattcgcgtg taagcgaacc gttgtcaatg
     1441 ctgttcttat aatacggata caaatattgg tcaaaacgtc cggcagaaat tgagtgaccg
     1501 cttgattcaa tttgaagtaa catctgtata aaccaaaatg tctgaccagc ctcatagaag
     1561 tttcttgcag gattttcagg cacgtttgca cagttgtttg caattacaag caatt
//
LOCUS       DAWACS010000339         1608 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_7515_length_1608_cov_2.160335, whole genome shotgun sequence.
ACCESSION   DAWACS010000339 DAWACS010000000
VERSION     DAWACS010000339.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7515_length_1608_cov_2.160335"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          452 a          390 c          228 g          538 t
ORIGIN      
        1 attctttctt cattgcaaga ccttcttcaa tatcaacatc aataatttca tgttgttgca
       61 tacatacgat tctgttctgc ttaccttcaa gtaaaagctc aactgcctta caacccattt
      121 cacttgccac aactctgtca cgaactgtcg gactgccgcc tctttgaacg tgaccaagaa
      181 tagtagctct tgattcaatg cctgtctttt cttcgatttc tttagccatt tcaataacac
      241 cgccgatacc ttcggcaacg ataataatag agtgctttct tcctcttgat ttactctcaa
      301 gaataggacg aagtacgtct tcgtcaaaac tccactttct ttcaggaaca agaataacct
      361 cagcaccgca agcgatacct gcctgaatag caatataacc tgccgctctg cccattactt
      421 cgatgattga acatctttcg tggctctgtg ctgtatctct gattttatcg actgcgtctt
      481 taacagtgtt aagacaagtg tcgtaaccaa ctgtatactc actgcaactg atgtcattgt
      541 cgattgtacc cggaagtgcg atagtcggaa gacctgccgc acataaatct cttgcacctc
      601 tgaatgaacc gtcaccgccg acgacaacaa gaccgtcaag accgaatacc tttgcaatct
      661 caacagcttt ctttacgcct gcctcttcct taaattcaag acaacgtgct gtttgaagaa
      721 tagttccgcc tctttgaaga atttcagaaa cagatcttgc attcatttct tccaattcac
      781 cgtttataag accgttatat ccacgtctta cgccaaagac tttaagtccg taatatgaac
      841 ctactctgac aaccgaacga attgccgcat tcattcccgg tgcatcacca ccactggtta
      901 aaacacctat tgttcttaca tttttgttct ccataatttg tcaatcctcc cacgaatttt
      961 taaaacagtt cgttttcaaa aattaaatcc tgtgctgttt ccagctccgt actcggtaca
     1021 agtacctcgt aacaactact ctcatttcca ttgcagttat ttgtgcgtct taatttggaa
     1081 attactttat tcgtcaaaag tacatctata agcttttttg ctgtttcgat tgactgcgat
     1141 acataaatta ctgtccacat aaaataaacc tcctgccgat aaggcaaacc ttatctcgca
     1201 tctgacatta gcttaccatg tttcgtgtat atctcagcag caccgttaat ctttattgct
     1261 gttgtaacat ctgtgaattg agcaagaaat acctctccct tatcaagttt ctccgtatga
     1321 tgaaactttg tatccttgcc gcttgtaagc cctataatat tcacaccgtt ttcaagtgct
     1381 tttactacaa tatactcatc cataattaat acctcgtttc ggtaattttg caaaatacta
     1441 catctataat ataatatagc atttcgcaaa atatttcaag agtaaataca ataaaatttt
     1501 taattatgca agtttatgtt ttcgtgaata ttgctacttt attttaacat tatcctctcc
     1561 gaagatattt tttaagtcat aaattgcatt ttctgtaccg ttaaacca
//
LOCUS       DAWACS010000340         1599 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_7566_length_1599_cov_1.812176, whole genome shotgun sequence.
ACCESSION   DAWACS010000340 DAWACS010000000
VERSION     DAWACS010000340.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7566_length_1599_cov_1.812176"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          478 a          297 c          291 g          533 t
ORIGIN      
        1 cgtcatgtaa gacgtatgcg taaattgaaa acgaatttta atcctgagga tttttcactg
       61 taacagtgct tgacatgaca tttgttaagt gttataattc aaatagagag taggagcagt
      121 aaaacgattg tttctactca tgtagtttgg ttttagtaga agaaatctac attcaaccgc
      181 cctatgccag tagggcggtt atttctttat tacccataca ataagcaaaa tcaacaataa
      241 ttgtaccgtt gtttcactca tagctgaatc ctttccgaaa caaccgccta ccgctcaaac
      301 tcaatatccg agccgttagg ctgtttataa tatatcatat ttttcggtta tgtgcaagca
      361 aaccgctttt tttaaaattc ttttccgttt aaaatttcaa cgtaatcttt ataattttcc
      421 tccaaaagtt ttggggtatc aagtccgtaa ggacattttg atttacattg attacagtgc
      481 agacaatttt ctatcttttt cattttagcc tgaacagccg gagtaagctg aagttcggac
      541 ggtgaacgtc ttagaagaag tgacattctt gcacagttat ttatttcgat accgacagga
      601 caaggcatac aatatccgca accgcgacaa aattcgcctg caagcatttt tctgtcatta
      661 tcaataagtt ccttaatttc acttgtcaca gacggtgggt ttgtgttgta actcaaaaat
      721 tcgttaagct cactcatacg ctgaacaccc caaataggaa gtacgttatc atattgtgcc
      781 ataaacgcat aagcggcggc ggaatttgat ataagaccgc ccgaaagtcc tttcattgct
      841 ataaaaccga gattattctt tttacagttt tcaactatcg caatatcttt atccgttgca
      901 agatagcaga acgggaactg aattgtgtcg taaagtacgg agtcgatagc ctcttgtgcg
      961 actgcaagac ggtgatttgt aagaccgata aaacgaattt ttccttgtgc ttttgcctca
     1021 agcattgcct cataaagtcc tgtaccgtcg ttcggtttcg gacagaatga cggattgtgg
     1081 aattggtaaa tatctatgta atccgttttc atattgttaa gacttgtatt taggtctttc
     1141 caaaaatcat cggcattttg tgcggcggtt tttgtggcga tgtatatttt gtcacgaaca
     1201 tccgacagcg cataaccgat tttttcttca ctgtctgtgt aaaaacgtgc ggtgtcaaaa
     1261 aaattaattc cgctgttata cgcatgaaga agtatttctt ttgcggaatc aaagcttata
     1321 cgttgtatcg gcaatgcacc gaatccgttc ttacagactt taatttctgt ccttcctaaa
     1381 gttacataat ccatatgttt tcgcctcttt aaatcaagtt ttcttaaatt atatcatata
     1441 gatttaccac attcaacata aaaagcaatc tttgcaatat aaaagacaat tattgaataa
     1501 caaaaatgtg tgtgatgtgg taaaatagaa ataacattat aatggaggaa ttttgtttat
     1561 gaaagagggt aaagttttct gttttcatca cggctggca
//
LOCUS       DAWACS010000341         1593 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_7605_length_1593_cov_2.239922, whole genome shotgun sequence.
ACCESSION   DAWACS010000341 DAWACS010000000
VERSION     DAWACS010000341.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7605_length_1593_cov_2.239922"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          471 a          317 c          303 g          502 t
ORIGIN      
        1 tcacggttat gatgtcgagg accttattaa aggttttatc cgcgacaaaa ggtttggctt
       61 tgaagaagtt acatatttac ttcttttcgg cgaacttcca aataaaaatg agcttgatat
      121 tttcacaaag acactttcac atatgcgaac tattccgccg tcatttgtgc gtgatattat
      181 catgcaagca ccgagtaagg atatgatgaa cgcactttca agaggtgtgc ttacgttgta
      241 tgcttatgat gataaagccg acgatgtgtc acttccgaac gttttaagac agtgtttgca
      301 gttaatcgcg caattgccgg tgttgtcggt ttacggctat caggcattta agtattatca
      361 tgaaaacgac agttttatcg ttcatgcacc taaaccggag ctttcgactg cggaaaatat
      421 acttcatatg ttgcgtaacg acagtcaata tacggagctt gaggcgagaa ttcttgatat
      481 tgcgttgata cttcacgccg aacacggtgg cggtaacaac tcaacattca ctactcacgt
      541 agtcacttca agcggtacgg atacctattc aactgttgcc gcgtctttgg gtgcgctgaa
      601 aggtcctaag cacggcggtg ctaatattaa ggttacgcaa atgtttgagg atatgaaaaa
      661 gaatattaaa aactggaatg acgataaaga gattgagcaa tatctttctg atttgcttaa
      721 taaaaaagcg ttcgataaat ccggacttat ttacggtatg ggtcacgcgg tttattcact
      781 ttccgatccg agagcgaatg tttttaaatc atttgttgag agcctgtcga aagaaaaagg
      841 cagagaagac gaatttaatt tgtattcaag ggttgaacga cttgctccgc aggttatcgc
      901 aagcgaacgc aaaatgtata aaggcgtcag tgcaaacgtg gacttttatt cgggcttcgt
      961 ttacagtatg cttgacttgc cgatggaatt gtatacacct atgttcgcaa tcgcaagaat
     1021 tgcaggttgg tcggctcaca gaattgagga acttgtcaat ccgggtaaaa tcattcgtcc
     1081 cgcatataaa agtatcgcac ctcataaaga atatgtccct cttgacaaca gagattaaaa
     1141 ataaactccc aatgcattgc attgggagtt ttgtattaat atcaattatt cataaaatct
     1201 gcaattttat caatcaatgt tatgtcagcc ggtagccaat ttacaccata taattcatct
     1261 ttattcaacc atttagagtc ttccgcctct ttcaaaataa tatctccatc tactattacg
     1321 cataaaaagc atttcatatt caaatgaaat ttaggataat catactcaat ggtatcaatc
     1381 aacttaccca ctttaatttt tactgtaagt tcttcttgaa tttctcttat taatgcctgc
     1441 tgtggcgttt caccgacttc aatttttccc ccggggaatt cccattgtcc tttgaactca
     1501 ccatatcctc tttctgttgc aaacacctca gttgtatttt caaaagaatc aacaataact
     1561 gccgctgcta cttctattgt tttcataata cta
//
LOCUS       DAWACS010000342         1592 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_7614_length_1592_cov_2.010410, whole genome shotgun sequence.
ACCESSION   DAWACS010000342 DAWACS010000000
VERSION     DAWACS010000342.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7614_length_1592_cov_2.010410"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          563 a          258 c          354 g          417 t
ORIGIN      
        1 ctttcggata tacttaatgt aataggaaag aacggtatcg acatcagcgc attgtcgatt
       61 gcggatacta ctgatttcgg tattgtgaga atgattgtaa acgacccgga taaagcggct
      121 gaaattctaa agagcaataa tcttgtcgta aaggttacgg acgttattgc acttgcggtt
      181 gctgataaac cgggcggact tgcaggtgaa attgaaaaac ttaaaaatgc aggtatttct
      241 attgaatata tgtacgcatt tatcggtaag agcgacaagg gcgcacttgt aattgttcgt
      301 gttgaaaatc ccgaaaaggc acttgaagtg cttaaagacg aaaatgtaac ggttgtttca
      361 cctgaagaag tttacagaat gtaaaacaac ttttgcaaaa taacgataat ttaagagcag
      421 taatctatga ttactgctct ttttgtatgt aaaaatgcac aaataaacaa tattaaaata
      481 tacaagctgt acagttgtca caaggaaaag caatttttgc aatatttaaa acaagatatg
      541 caattcttta atgtttaaaa tttgatataa ttaaatcaat cgttaaagca tataaaagag
      601 gaggaaatga gaaatgaaga aaaaaataag tattttttgt tcactgctgt tggcattaca
      661 gatgatgttg taattagcgg tagttccggt ttttgcagac gaagaactaa cttctgctgt
      721 atacgaagaa ccggaaagtc cggcggttac atataatatg aacgtcgact ggaagttcaa
      781 aaaagcggca gacggaagtg aatatccgct tgctaaggca tcggcaagcg tcgcaaaaga
      841 cggaaagaac ttttacgatg tagattatga tgacagtgat tgggatacgg tcagtgtacc
      901 acacccgata aatgctgaag attcattcga cagctcgtgt gatgacaaag gtgagggagg
      961 actttacaga ggttttatgt tttacagaaa gcatataaca attcctgaat cagacgcagg
     1021 caagaaactt ttccttgaat ttgaggcagt aagaaactct gtttatctat atgtaaacgg
     1081 cgccatagtc ggctactatg aagccggtat cgcagcaaca ggctttgata ttacagacta
     1141 tgttaaaccg ggtcaagata accttatagc agttgcaaca gataatgcgg ctgacagagg
     1201 tcaggctgat tcatcaaagg taacaaaaga aacaaaaccg ggaagcacac cgggtgcggc
     1261 agacggttcc ggttatcaat ggaacacaaa ggacttcaac gaagttcaag gcggtattac
     1321 aggtgacgta attctttacg caaagggtaa gacttatcaa acattgccgc tatataataa
     1381 cctaaaaaca aaaggtaact atatttatgc gacagacttt gatataagac aaaagtcggc
     1441 aacaatcaca gttgagggcg aagtaagaaa tgaagacaca gataaggacg ttactttgga
     1501 agtaaacgtt gttgaaatgg ttgaccctaa caaggactca acagacgttg acgcagacca
     1561 tacaaagcaa ccttatttga gatattcatt tg
//
LOCUS       DAWACS010000343         1573 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_7722_length_1573_cov_2.424901, whole genome shotgun sequence.
ACCESSION   DAWACS010000343 DAWACS010000000
VERSION     DAWACS010000343.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7722_length_1573_cov_2.424901"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          520 a          230 c          375 g          448 t
ORIGIN      
        1 tataaagaat ttctacgcaa gatatgtacc cagtgcaatg tagaagattc ctcaaataca
       61 gaagatttta attcttcttg ggcgtggaat acatataatc aagatatgca gactatggaa
      121 tataagacta ttttggacgg caattcatat gaactgaata ttctcggcaa tatggatatg
      181 ttcaaaagtg tggatataaa cgtgtataag gcggataaaa agagcggaaa ctatacatat
      241 ataggtaaat acagcgattt ggacggtgac tgggaagcag gtatttttaa agataatttt
      301 aacggcaaaa tgcttagatt atgtggtaaa aatatatcgg ttaatcttgt cggaaaatat
      361 gacggttacg agatttattc gactccgatt atattaaacg gcaaaagaag caatgttcgc
      421 attatgcacg ataccgaaaa agacagttac aaaattatcg gtgcttgggg cggtcttgat
      481 tcgacaaacg gcagagcata tacgaattta aaaaagattg gtttctttga taaaataact
      541 cctattctta cggtatatga cgtggaacac aactcaaacg acaatataac aggttcatgg
      601 actttaaaaa tgttcggcgg tgtgaaaaaa gcaaatattt cagacggagc ttatattttt
      661 gaatacgaac ttactgacat atacggtttg aaacgacgcg gtactgcggt taaagcgaat
      721 gcgggcggcg gaaacatcag atttgaataa agagggttta acaatgggtt atacagaaaa
      781 tttaatcgat tttatcggaa aaagtacgga ttgttaccat gcggtcaaaa ccgcaaagga
      841 caggcttgac gaaaacggtt ttacggaact tcttgagggt gacaagtggg atgtacaaag
      901 cggcggtaaa tattacgtta tacgaaattc gtcatcaata attgcgtttg aaatgccgaa
      961 aaaagatttc ggtgcgttta tgatttgtgc ctctcatacc gattcgccgt cattcaagct
     1021 aaagcctgat tttgaaataa atgcggacgg ttgtataaag ttgagtgttg agggttacgg
     1081 cggtatgata atgtcgtcgt ggcttgacag accgctgtcg ataagcggaa gaattgttac
     1141 ggcaaagaac ggtaaggttg aaacgaaact tgtcaatata gacagggatt tgcttgttat
     1201 tccgtcactt gcaattcata tgagcagaga tattaataag ggctatgaat ttaatataag
     1261 acgtgatatg tgtccgatag cgtcggagaa aaacggcaga attgaggata ttattgcaga
     1321 aaatgtcgga gtagataaag aggaaatatt gggttttgat ttgtcgcttt acaatcgtat
     1381 gcgcggtact gttttgggcg cggacggaga atatttttcc gcaccgcgaa ttgatgattt
     1441 acagtgtatg tacagtacac ttgagggatt tttaaattcc gaaagtgatg ataatgtaac
     1501 tgttttcgcc gcgtttgaca atgaagaagt cggcagcggt acaaagcaag gtgctaaatc
     1561 gtcatttttg cgt
//
LOCUS       DAWACS010000344         1573 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_7724_length_1573_cov_1.856390, whole genome shotgun sequence.
ACCESSION   DAWACS010000344 DAWACS010000000
VERSION     DAWACS010000344.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7724_length_1573_cov_1.856390"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          403 a          371 c          238 g          561 t
ORIGIN      
        1 aatcagttgc tctaccaact gagctacacc agcgaaaatg aaatataaga gagagccgaa
       61 gctctctctg taatattttt agttaaacaa acttttagca atcgtgctga ctgcttttcc
      121 gtctgctctt cccttaactt tcggcataac gtattccata accttaccca tatctttcat
      181 ggaggttgcg cctactgctt taacgccttc ttctacaata ggacgtagct cgtcctctgt
      241 aagctgttta ggaagatatt ccatcaagca ttccatttcc tgttttaact gagagattaa
      301 atcctctctg ccactctttt catattcagg aagagagtcc ttgcgttgct ttaattgctt
      361 tgcaattacc tccaaaatat cctcgtcatc aagagttact ttgttgtcct tttcaacctg
      421 caatattgca gtacggacaa gttgtactgt atttttgcgt acagtgtctt tgtttttcat
      481 tgacgatttt agatcttctg ctaatttttc ctttaaagac attatcgtca tctccttatg
      541 ttgctaatta aaaattagaa ctttcttttt cttgcagctt ctgatttctt tttacgcttt
      601 acgcttggct tttcatagtg ctcacgcttt ctgatttcag acatgacacc tgacttagca
      661 cattgacgct tgaatctgcg aagtgcgcta tctaatgatt cattatcttt tactcttact
      721 tcagacattg tttcccctcc ctccggcaag tcaattctaa aaacggactt gatggactca
      781 gttatacact gtttgtacaa caatatctat tataccttat tttacataga tatgtcaaga
      841 tattttagtg atttttataa agatttgttt atttttttag ctgccgacaa atttatcggc
      901 agctgtattt attatttcaa tgtttttgac aaacgataca tcactacgca ggtccaaagt
      961 tttgtatcat caagccccaa tccgctgccg gtgccgacaa ttataccgtt atccacgcac
     1021 caccgaacag cctcttttgc ccagtcgggc atattatcat cgatctaatt ataaatcata
     1081 ggattttagt ttttgtaaat ctcattatgc aaaacatcaa ctcttgcagt ctgttccgaa
     1141 atcatttgtt ttaattcttc atattgcgac attgtaagtt cctcctccat tttttcgccg
     1201 actatcgcgt cgcttattgc ctcggcaatt ctgtctgcgc cgacttcatt atataaatcc
     1261 gcgtccgatt cggtgtcgac aaagcacact tctataagca tcgacctcgc attacttctg
     1321 cgaatgacat tcaaatttga gccgtttttt atacctctgt ttataaaccc aagctccgcc
     1381 atattttcac ataccagctc cgcttgttta aaaacttctc cgttataggt ataaacctca
     1441 gttcctctgc cgccgccgct gttaaagtgt atcgaaacga acaaatcaag cggctgtgca
     1501 tttgctttat caacaatctt tttcaaattt ccgcttaccg tagaagaata atcatcagtg
     1561 caatcaacca ccg
//
LOCUS       DAWACS010000345         1570 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_7734_length_1570_cov_1.972937, whole genome shotgun sequence.
ACCESSION   DAWACS010000345 DAWACS010000000
VERSION     DAWACS010000345.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7734_length_1570_cov_1.972937"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          434 a          375 c          231 g          530 t
ORIGIN      
        1 gatatttacc tctgaaatat tatatacacc aatcccaata accgtccttc atagacgcat
       61 ttccgacact gtactcaagt tcgggatttt tcacgctgac tttcataccc tccgaaacgg
      121 aattaacgat aaacgcatta ccgccgatag tcgtaccctt accgacaacc gtttcaccgc
      181 cgagaatagt cgtacccgaa tatatcgtaa cgttgtcact gatagtcgga tgacgtttaa
      241 cgccctttaa ctgctgacct tttcttgtcg aaagtgcgcc taaagtaacg ccttgatata
      301 tttttacatt atcgccgatt tcggtagttt cacctataac aatacctgta ccgtggtcta
      361 taaagaaata tttgccgaca gtcgcacccg ggtgaatatc tatgcctgtt gtgctgtgag
      421 catattccgt cattattctc ggaatcatag gcacgcccaa aagccacaat tcgtgagcaa
      481 ttctgtaaac agtgattgca aacactcccg gataacagaa aattatttca tctgtgctgt
      541 atgccgcagg gtcaccgtca tacgcagctt gaatgtctgt atttaagtat tctcttattt
      601 tcggtatttt ctttaaaaat tcaaaaacaa cttcttccgc tttttcctct gcctgtgcct
      661 tactgcactc tctgcataca tcttgatttg caaacgcgtt tgatacctgt ttcttcaaat
      721 gatactgtat aaattccagt ctttcaccga caagaaactt taagtattca cttctgacct
      781 tgttgttgtc aaaaaagccc ggaaaaagca cttttcttat ttcatctata atttctataa
      841 gaatatcctt gttaagcata ttctcttcgt caattctcgc tgtaatgtca cattcattgt
      901 agctgtctaa aatcacattg actaaatctt ctactttatt cgccatatgt aaacctcctt
      961 atgtactatt ttatgcactg ttcctatagt ctatcacaca tatcgaaaat tgtaaactaa
     1021 atttcaggtt ttgtcaccgc gtcgggatta accttatgtt cgccgataaa tttctccatc
     1081 caaatcatat cgtaccactt gtcgaacttg taaccgcact ttttgaatgt gccgataagt
     1141 ctgaaaccca tatgctcgtg gaactgcata ctgttgtttg tcaaatgccc atcttcattg
     1201 tccgtatacg caataaaagc atacatatta agaatgtttt gttttttcaa tctttcttca
     1261 agttcaagat acaacttctt gccgtatccg ccaccttttt tgtcctctct tacatatata
     1321 gtcgtttcaa cgcaccaatc atacgcctta cgtcctttga acgttcccgc atatgcataa
     1381 ccgacaattt catcgtcctc aaccataaca atgtaaggat acttttttga aatattttca
     1441 atacgacttc taaactcgtc tacagacgga gtttcatatt caaacgatat tgttgtattt
     1501 tcgacatacg gacggtatat ttcaaccaac acttccgcgt cggataattc aacatttctt
     1561 atataactca
//
LOCUS       DAWACS010000346         1564 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_7770_length_1564_cov_2.027833, whole genome shotgun sequence.
ACCESSION   DAWACS010000346 DAWACS010000000
VERSION     DAWACS010000346.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7770_length_1564_cov_2.027833"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1294..1303
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          385 a          329 c          296 g          544 t
ORIGIN      
        1 aagcctgttc tgaaagcaac attatcaagg cgggtttcaa tcattgtaag aagaactgca
       61 cctgtctgtc cctgagcttt agcagccttc tcataatagg tgtggaactg cttttcaaga
      121 atgccgtaga taaacttaac tttctgcttt tcgttaagct gcatagcata ttcgctcttc
      181 ttacggcgat tcttactata agaatttcta aatgtatcct tatttgtata acccataaca
      241 gccggtgaaa taccgagtgc tttgcaacgc tttgcaatag gttctgaatt ctttgccata
      301 ttttactaca cctccaataa actatacacg acgtcttttt ggtggacgac agccgttatg
      361 tgggatagga gtaacatctt tgataagact tacttctaag ccggctgttt gaagagctct
      421 gatagcagat tctctacctg agccaggtcc cttaacgtat acttcaacat atttcatacc
      481 atgttccata gcagcctttg cagcggtttc agcagcagtt gcagcagcaa atggagttga
      541 ttttcttgaa ccacggaaac ccatttctcc ggcagaagcc caagaaacag cattaccgtt
      601 catatcggta atagttacga ttgtgttgtt gaaggttgat tggatatggg cagcaccacg
      661 ctcgatgttc ttcttctcac ggcgtttgcg tgagcctgtg gtcttcttag tagccatact
      721 tatttgttcc tcctactatt tcttcttgtt tgctatggtc tttttaggac ctttgcgtgt
      781 acgagcattg ttctttgaag tctgacctct tacaggaaga ccctttctat gtcttacgcc
      841 acgatagcaa ccgatttcta taagtctttt aatgtcaaat gctgtgtttc tacgaaggtc
      901 accttcaaca gtgaggttgt gggtgatgta gtcactgagc tttttaaaat cgtcttctgt
      961 taagtctctg caacgggtgt caggattgat gccagttgct ttgatgattt cgttagaggt
     1021 tgtgcgaccg ataccgtaga tataggtaag gccgatttca actctcttgt cattaggtaa
     1081 gtcaacacct gaaatacgtg ccattttaca gtttacctcc gattatttat tcaggattag
     1141 ccctgacgct gtttgtgctt tggattttca cagataacca ttacttttcc attgcgctta
     1201 attactttgc acttttcgca aattttcttt acagaaggtc ttactttcat tctgaatatc
     1261 ctcctaatta ttactttgag cgccatataa tacnnnnnnn nnnccttttg taagatcgta
     1321 tggggacatc tcaatcgtta ccttgtcacc gggaagtata cgaatattat tcattctgag
     1381 cttcccactg atgtgggcta taatgatgtg gtcattttgt aatgttactt taaaagttgt
     1441 attaggtaac acttcaacca cggtaccctc aacttctatc atatctgact ttgacatctt
     1501 atacctcgct aaaattttat tatttacgat tgatgttttt ctatttaact ggaatcacat
     1561 tacg
//
LOCUS       DAWACS010000347         1561 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_7788_length_1561_cov_1.938911, whole genome shotgun sequence.
ACCESSION   DAWACS010000347 DAWACS010000000
VERSION     DAWACS010000347.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7788_length_1561_cov_1.938911"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          510 a          253 c          368 g          430 t
ORIGIN      
        1 ataacaccgt aacagcacgt tgataagatt gcggggcaga ttaaggatat tgccgatatg
       61 cttaatatca gctatgataa atttatccgt acaaccgacg attatcacgt taaggctgtt
      121 cagcagattt ttaaaaagct ttatgatcaa ggtgatattt ataagagtga atatgaaggt
      181 tggtactgta caccttgtga atcattctgg actgaaacac agcttgtaga cggtaagtgt
      241 cctgattgcg gcagagaagt taaaaaggct aaggaagaag cctatttctt caaaatgagt
      301 aagtatcagg acaagcttat ggaatacatc gagtcacacc ctgaatttat tcaacctgaa
      361 tcacgtaaga atgagatggt taataacttc ctaaagccgg gattgcagga tttgtgcgtg
      421 tcaagaacga gctttacatg gggtattcct gttgagtttg accctggaca tatcgtgtac
      481 gtgtggattg acgcactttc aaactatatt acggcccttg gctatacacc ggaagaaaag
      541 ggcgaattgt ataataagta ttggcctgca gatgtgcata ttatcggtaa ggacattctt
      601 cgtttccata caatttattg gccgattatg cttatggcat tgggcgaacc gcttccgaaa
      661 caggtgttcg gacatccttg gatgcttgtc ggtgatgaaa agatgagtaa gtcaagaggt
      721 aacgtaattt atgcggagga ccttgtaaag catttcggcg ttgacgcggt aagatattat
      781 tcacttcacg aaatgccttt cgcacaagac ggtacaatca cttatgaagt gtttatcagc
      841 cgatttaaca gtgaccttgc aaatacactt ggcaatcttg taaacagaac tgttgcaatg
      901 attaataagt attttgacgg tgaaattcag agcggtgacg tacacggtga ttttgatgat
      961 gatttgaaag ctgttgcaac aggtgcgatt gataagatta ttaagaagat gaatacactt
     1021 cacgttgcgg atagtatgga cgaaatttgg aaagttgttg acagagcgaa taagtatatt
     1081 gacgaaacaa ctccatgggt gcttgcaaag aatgaagacg ataagccaag attgggtaca
     1141 gtgctttata atcttgttga aacaatcaga attattgcgg cattgttaag ttcgtatatg
     1201 cctgaaacat caaagaaaat cgccgaacaa atcggtgcag atgatttgag ctttgaaagt
     1261 acaaagacat tcggtgaaac aaaggcgggt acaaaagtag gcgaggccgt tccgctgttc
     1321 caaagaattg acgcagaaaa gaaacttgcc gaacttgaag aagaattcgg tcagccggaa
     1381 gaagaaaaga ttgaaattgc accgtataaa gattatatcg agtttgaaga atttgaaaaa
     1441 cttgatattc gtgtcggtaa ggtacttgaa tgcgaaaaag tgccaaagtc atcaaagctt
     1501 cttagattta cattgcaagt cggaagtgaa acaagacaaa ttctttcagg tatagcaaag
     1561 t
//
LOCUS       DAWACS010000348         1559 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_7802_length_1559_cov_1.788564, whole genome shotgun sequence.
ACCESSION   DAWACS010000348 DAWACS010000000
VERSION     DAWACS010000348.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7802_length_1559_cov_1.788564"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    665..674
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          454 a          254 c          321 g          520 t
ORIGIN      
        1 atttatcacc gttatttttt atataattat atgcgtccaa acgttcttga gtcggacgga
       61 ctgtaatatc atattcggct ttggcaaatt tatcgagaat atcaagaaga tgatttttta
      121 tttgcccttt acctgaatgt ggattaaaaa taaaaagcat tttctttgtc atagaaatca
      181 cctcaaactt ttaagtattt tatatataaa ttataccttt ctaaaagaaa atagtcaaga
      241 aaatacttgc cattaatgta gtttgcgtgt ataatattac tattatgcaa caaataggag
      301 ggaaaataat tgcatatagt aaaggaaaag agattttaca ttaatctgct cacaataaca
      361 ataccgattg cactgcaaaa tcttatcagt tttgcgacaa gtatgatgga tacgattatg
      421 ttgggacgag cagacgatac gggtgtgttt ttgtcagcat catcgcttgc aaatcagccg
      481 tttttcattt tatcgcttgt ctgtttcggc ttggcgggtg catcaacggt acttagtgcg
      541 caatattggg gaaaacgtga tactgcgtca atacggtcga tttattcgat tatattaaaa
      601 gttgcgtttg tcatttcaac acttatggca cttgcggtac ttatatttcc gcaagccgtg
      661 atggnnnnnn nnnnccgtga tggggatata ttcaaacaat gatgaaataa tcgcggcagg
      721 aatatcatat ctgcgtatta tcggttttgc gtatattttg tttggtctaa gcaatacgat
      781 gatatgctgt ataagaagta tagagcttgt gaaaatatct gttgtggtaa atattacatc
      841 gttctgtaca aacgtatttt tgaattgggt gcttattttc ggtaatctcg gagcaccggc
      901 gctgggtatt cgcggtgcgg cgattgcaac tctcacagca agaatacttg aatttttgat
      961 agtaagtata tacattttct gcattgacaa acgtcttaat tttaaaccta agcatttgtt
     1021 cttgtttgac aagatatttg cgcttgactt gtttcaacac ggattgcctg tatttataaa
     1081 tgaagttatg tggtcgctcg gtgtatcagc acaagcggca atactcggac acattaaata
     1141 ttcagccggt gaccccgttg cagccaattc aatcgcgtca atggtgcaac agctttcaac
     1201 ggttgttata ttcggtatcg ctaatgcggc aggaattata ataggtaaat ctatcggttc
     1261 gggaaaaatc gaaagggcaa aggttgaagc aaaaacatta aaatatttaa gctatgttat
     1321 cggcgttatt gcctgcggag ttatattgct tttgaagaat tttgttatca atttctacac
     1381 aattccgccc gaaacaaagg cacttgcaaa tgaattgatg gtaacgattg cgttcataac
     1441 tatatttgta tcggtttcat ctgttacgat tgtcggaata cttcgcggcg gaggagatac
     1501 aagattttgt atggttgcgg aaattattac gctttggtgt gttgcaacac cgcttgcac
//
LOCUS       DAWACS010000349         1553 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_7833_length_1553_cov_2.465955, whole genome shotgun sequence.
ACCESSION   DAWACS010000349 DAWACS010000000
VERSION     DAWACS010000349.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7833_length_1553_cov_2.465955"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          441 a          325 c          267 g          520 t
ORIGIN      
        1 ttactaagtg tattaaaatc tcctgttgat acctctgttt gactaaatct tctgcattca
       61 atttgtgtgc ctacaaaaga aattgctgag aatgattcga catagctatg tgtactgctt
      121 atagtcgtac ctatatgctg tataaatcca ccaaacatag taccgttgca cgcatagaaa
      181 acaccgttgc taagagtatt gttactacca ctcggaaata aaatgcccgt gttggtaagt
      241 tctacagtac actccctgaa tacccaccaa caaacgtgac tactatcata tatctttgca
      301 ggcaatgtta ctgttatttt actgttgctc aactttacat tacaattttt aaatagtgaa
      361 tctgtattat gtagtgtgtt tgcatatgta attgaacaat tatcaaactc caacgaggta
      421 tagtcgcaaa aaacaccaac atcaggggac caattagttc tacaagcaaa attaatatcc
      481 atattcttaa acactgcggt tgcgcttgtt tcagatgttt tagatataag aaccttatta
      541 cttgtattga ttttcgtaat ccctttgcct tgtccctcaa ttataatatt tttactatgt
      601 gtgagttgag ttgacaaatt atatgtgccc tctaaaagaa taattttgcc accactattg
      661 ggtagcgaat caatagcttt ttgtatgaca atccctgcat cttcatattg gttatacata
      721 tagtccacat atcttcctgc cacctcatca gttgtcgaac ctatagtgat aacagaagaa
      781 tttctaccac ctgatgtata acctgatgat ccgacaataa cccaattcgt accgtcatat
      841 acaagctcta aactctcaaa agcactccaa gattttgatg aggttattgg tgcgtagctt
      901 gcgacatcgt tatcaaaaaa tgcacgagca ccgaatttta taataggttt agctccggta
      961 ttgcttacgt ttagagttgc tccttcttta gaggtatgtg acgaattaaa tttaacaaca
     1021 attcttgcac ctataaccaa tctaaagttt gatatagaaa cagttttcgc cgccacatta
     1081 ccctgagtgg tacattctgc ataaggtgga ccttgccaca ccggtgcgcc acttccgcta
     1141 cctatcaagc tatatcctgc cgttcccgca cttgtcggcg cataccacga cttgcttgct
     1201 gttgccgaac cgttataact tgtagccgat ccattcattg tcagtgtcaa tgaattagga
     1261 ttttgcattg atgtaggctt gttggatagg tcagtatatg accctgtaaa tgctactgtt
     1321 tttaggtcgg caaaaaattt ctttatcttg ccaaacaatg tacttaattt ttcaccgctt
     1381 gctatattgg ctcgtgtgct tgcctcagta aatgtcggcg tttggtcgtt ggttgcaaca
     1441 tttggtacat tgtccaatcc aatctgttct gctgtgacat catgcggatt ggattgattt
     1501 gcaatatgac taatgaaatc tgctattgct ttcataattt ttccaaacga aac
//
LOCUS       DAWACS010000350         1553 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_7831_length_1553_cov_3.331776, whole genome shotgun sequence.
ACCESSION   DAWACS010000350 DAWACS010000000
VERSION     DAWACS010000350.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7831_length_1553_cov_3.331776"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          365 a          366 c          294 g          528 t
ORIGIN      
        1 gcaatataac acagctgtaa tttgcggcag cattgataaa cgccgccttt ttgattgata
       61 tatttttaac atcatttttt atcatccatg tttcaactcc ctgtatataa tatatacact
      121 aaaattcaac ttgtcaaaca tattttttct gacaaaaaaa gacagatgaa acggtttaaa
      181 tttcatctgt ctttttatca gtccatagtt acagtaggcg gaatttgtcc gctgtaaaca
      241 ggtacgtcaa acgataatgt tgcgtctgtg aatgacgaga atatcttttc aatgcttacc
      301 gcctgcttaa ccgcccagcc aatatccgtt gcatattggt gagtacccgg attttcaggg
      361 ttccaaagca ttttataaag tgtgttctgt ctgccatccg aactgttcac atagttttcg
      421 cttatccact ttgcaccgcc cttaatagcc gcctctacac tcgtccagcc ttgactgtat
      481 gctctctgtg aaccgtttcc gacagggttt gcgtcatatg caccgatacc gaacaagtta
      541 taaacggttg tgccgttgac ctcaacaccg gttgcaagct gtgaagtacc atttccggat
      601 tcaaggcaag catgtgcaac aaggtaaact tcgctgacgt tatattcttt agccgcctcg
      661 atgaatgccg ctcccattcc tttcattacg cccttatcgg caagataatt attaagcgtt
      721 tcttcgctga cattattcgg ttttgaaagg tcaaggaact gatatttata tgcgtccgta
      781 tagaaactgt tcggattcat atattccgct gtttcggact ctgtcgcata tgacattccg
      841 ccgtttctcc aaattttcgg cggtgggttc aagcccatct gtattcttat aatctgtggg
      901 aatgatatgt catatgacgt gtatgttaca gttccctccg tatccaaatc agaaacttca
      961 tcattcaaat tgaatgggtc ttttgcgtcc gattcggtta cgttgacagt aaacgtctgt
     1021 ttctttccgt tagtcgtttc aaccgttata ttcgccgtac caacgctgac accgcgtatt
     1081 ctgcccgctt gacttactgt cgctatcttt gtatcgtcac tcgaccattt aagcgttttg
     1141 tttcgtgcgt ttgacggtga aacggttact atcgcgttaa cactgtcgcc gacttttatc
     1201 gtcattgcgt cagtattttt tacggataaa gtctgtacgt caacgtatga atttacgaca
     1261 tttacaaagc acttcgcctc aaagcctccg tcctccgttg ttgccgttat ctccgtcata
     1321 ccgacgccga caggctttac tctgccgttt tcgtccacac gtgcaatctt tgtattttta
     1381 gatttccatg ttatgttttg gttcgttgcg tttttcggga atatttctgt ctgtaaaagt
     1441 ctgccctcac cgcctgtata aagcgttatt gtgcttgtcg gcataaatat acccgttaca
     1501 ggttgtctta ccgacatagt agcggttgca ctctcttttt gactgtacaa cat
//
LOCUS       DAWACS010000351         1550 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_7856_length_1550_cov_1.713712, whole genome shotgun sequence.
ACCESSION   DAWACS010000351 DAWACS010000000
VERSION     DAWACS010000351.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7856_length_1550_cov_1.713712"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          428 a          307 c          189 g          626 t
ORIGIN      
        1 tttcccgact gagtgtagtt ttcttttcgg ttgataatcc tatacattcc ttttcaatta
       61 actcaagccg ttcattataa ttactgagtt cggaattgaa actgccactt aaaattttaa
      121 attgttcatc acttacaatt ccgcttaatt tatctttata aagctgtaca gagtcatttt
      181 gtaattttgc gattttattt tcaatttgtc tcttttcttc ctgcaatgtt gaaagccgat
      241 tttgagaatc tcgtttcttt tgaagtttta tctcgttttc atcaaaatac gtttcagaca
      301 ttttatgcaa ttcttttaaa atcaaatctt ctaaatcaat ctgcctgata ctgtgtgtat
      361 tattgcatac tccagtgcta ttctttactg tacgacatct gtaatactta tatcgttgtt
      421 tactgcattg ataactcatt ctccacattc ccgaaccaca ttctttacaa aatacaattc
      481 ctcgaagtgg atttatttcg tatgtattgc ttttaaactg cttatcattt ccctgtcgat
      541 tttttaagcg atcttgcact gcattccata attcttcatc tattatcggt tcatgcatat
      601 tcggaacaac tatccattca cttcttggta atttaattcg tttcttattt ttataagaaa
      661 tatttgttgt tttaccttga acgattgctc ctgtatacac ttcttgacgt aatatataat
      721 atatagtgct atcacgccat tgccttgatg aaacattttc agcattcgga tttcgataat
      781 ttaacccttg caattctttg tacacaccag gaggtaatat ttgtttttca tttaaggctt
      841 ttgctattcg tatatatccc ataccctgtt tgtacatttc aaaaatactc ttaacaactt
      901 ctgccgccgg ttgatcaata ataaatttat gcttatcttc gggatttaat ttatacccat
      961 acggtgcata aggacaacaa tattcacctt ttattttctt atggtgcaga gttcttcgta
     1021 tgttatctga taaatcctct aagtaccatt cattgataag tccatttatc tgacgtgctt
     1081 tcttatttcc ggcaacattt gtatctgcgt ggtctatgat acttacaaaa cgtacattcc
     1141 attcaagaaa tttaccatgt atgtatttct ctatcatttc catatccctt gaaaatcttg
     1201 attgtgtttt acacagaaca atgtcaactt ttccttcttc gcagtccttt aacatttgat
     1261 taaatgcagg tcggtttctg tcagctccac tataatcttc atcgcaataa atattatgta
     1321 ttttccagcc ttgttcaata caataattta ttaacataga tttctgattt tgaatactct
     1381 cactctcatc tattacacac ctcttatctc tgtcctcatc agacaatctt acatatatat
     1441 ctgttaaatt tctcataact gttacctcct ataaataggt gtaaccgctt ttgtatgtaa
     1501 taatagtata acatacaaaa gcggtaagtt aaagcttatt ttcacaaaaa
//
LOCUS       DAWACS010000352         1549 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_7857_length_1549_cov_2.319946, whole genome shotgun sequence.
ACCESSION   DAWACS010000352 DAWACS010000000
VERSION     DAWACS010000352.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7857_length_1549_cov_2.319946"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          514 a          259 c          384 g          392 t
ORIGIN      
        1 ataaagtgtg cggtaaacca ctttcaaagt gggttattga cgcgtctaag gcggcaggtg
       61 cggacaaggt ttgcgcggtt gtcggtcata aggcagaaac ggttaaagag gtactcggcg
      121 atgtgtgcga atttgcattg caggctgaac aaaagggtac aggtcatgcg gttatgcagg
      181 ctatcgatgt tataaaaaat tcaaagggcg aggttgttat tctaaacggt gacactcctc
      241 ttataactgc cgaaacaata aacaaggcta ttgaatatca caaaaataac ggcaatcaag
      301 caactgttat aaccgcaatt cttgacgatg cgacaggtta cggaagaatt gtaagagata
      361 atgacggttc tgttttgaaa atcgttgagc aaaaggacgc aagtgaagaa gaaaagaaaa
      421 tcaacgaagt aaattccgga atgtacgttt ttgacgcaca gtcgcttgta tatgcacttg
      481 ataagattac tccgaacaac gcacaaggcg aatattacct tacagatact cttgaaatac
      541 ttctaagtgc cggtaagaaa atcggaggtt acgcaatttc ggacaatgac gaaatcagag
      601 gtataaacga cagagtacag cttaatgaag ccgagaagat tatgcaaaag cgtattaatg
      661 aataccatat gagaaacggt gttacaatgc gtaatcctga aagcgtttat atcgaagacg
      721 gcgttgaaat cggcaatgat actgaaattt gtcagaacgt cacaataaag agcggtacaa
      781 aaatcggctc tgactgcgtt atcggttcgg gaagtatgct tgacagagcg gttatacatg
      841 acggcgttga cgtgttaagc tcagttatac ttgaatcgga agttgacgag ggtacacacg
      901 taggaccttt tgcgtatatc agacctaatt gtcatgtggg taaggaagtt aaagtcggcg
      961 attttgtcga acttaaaaat tcaaatattg acgacggtac aaagatttct caccttacat
     1021 acatcggcga cagcgatgtc ggtaagaggg taaacttcgg ctgtggtact gtaacgtgta
     1081 actatgacgg taagaagaag tacagaacaa caatcggtga cgattgcttt gtaggctgta
     1141 atacaaactt cgtttcgccg attaatgtcg gtgacggcgt ttatattgcc gcaggttcga
     1201 caataacaga agatattcct gaaaacagcc tgtctatcgc gcgtgcaaga caggttaata
     1261 aagagggttg gaaggataaa cgtaagtaat atgagtttta aagatatttg ggcagataaa
     1321 ttatctgccc tttttggacg taaagaggag attataccaa tgtatttaat agtgggactt
     1381 ggaaatccgg gtaagcagta cgatatgaca cgtcataata tcggatttca cacaatagac
     1441 tatattgcgg ataagtacgg tgcgaaactt accaaattaa agtttaaagc ggtgtacggt
     1501 gaggcgacaa taagcggtga aaaggtgtac cttgtaaaac cgcagacat
//
LOCUS       DAWACS010000353         1537 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_7938_length_1537_cov_2.085695, whole genome shotgun sequence.
ACCESSION   DAWACS010000353 DAWACS010000000
VERSION     DAWACS010000353.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7938_length_1537_cov_2.085695"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          479 a          244 c          364 g          450 t
ORIGIN      
        1 atattattcg gcgtaatgtt tataccgcag gaactgaatt tgcttgcaaa cagccttaca
       61 tcgcttgcgt gcggtgcaca ggttgaaagt ttcagaaaga tacagggcaa cggtatggcg
      121 actacaatgt gcataggtaa cttgcgtatg gcaacgcaat cgatatgtga ttattggttt
      181 acaaaagaca aacccgcaat ggaaaaagga tttttgtttt tcggcataat tgcaatcttt
      241 acgataggtg cgattatggg taacgtatct gtcagattgt tcagcgaaaa ggctattttg
      301 gtaagcagta tattgctgat aacggcgttt tcgattatgc ttattaataa ggaagattga
      361 ggataatatg gaacaggaaa ttttaaaaat tgaaaatttg tattttgatt attccgatac
      421 atctgtattg cgtgatgtga attttacatt acataagggt gattttctcg gtataatcgg
      481 tgcaaacggt gcgggaaaaa gtacactgat aaaaataatt ttaaaaattt tgccgtacaa
      541 caagggtaaa ataacacttt tcggagaaga tttatcgaag tttaaagaaa attataaaat
      601 cggttatgtt tctcaaaaag caaattcgtt taacagcgat ttccctgcaa cggttaaaga
      661 ggttgtaatg gcaaatctgt acagcagacg cggacttttt aaacgctaca gaaaagagga
      721 ttacgaaaaa ctaagtacag tgcttgaaaa agtgggaatg tcgggatacg aaaaccgact
      781 gatcggtaat ttgtcgggtg gtcagcaaca gaggatattt attgcaagag cgttgatttc
      841 ggaacctgaa ttgttgctta tggacgagcc gacagtcgga gttgatgcga agtcggtaaa
      901 acagattatg tacataataa gcgaactcaa caaacaggga atgacgatta ttatgacaaa
      961 ccacgatact ccggaacttg tcagagtatc ggacaaactt ttaatattct gcgaacacgg
     1021 caacggagaa tttgtaagcc gaaacgacct tacaatgcaa cagataaacg aaatatttgc
     1081 aggaaagagg gtgcatcatc atggataata tttttcagta cgcatttatg caaagagcat
     1141 ttgtagttgc ggcaataatt gcggtgattg caccgtgtat aggcgtttcg attgtactta
     1201 aaagactttc ggcgataggt gacgcaacgt cacattcggc gctggcaggt atcgcattcg
     1261 gacttttgct cggaattaat ccgattttgg gcgcggtgat gttttcgatt tttgcagtta
     1321 tcggaataga ggttttcaga aaatcgttcg gtaaatattc ggaaattgca acggtggttg
     1381 ttatgtcggc gggtatcggt cttacggcgg tgttgtcggg atttataacc aacggctcaa
     1441 ccaacataaa cagctttttg ttcggctcga tagttgcaat aaccgatttt gaattgtact
     1501 taacgattgg actcggcata gcggttatta taacttc
//
LOCUS       DAWACS010000354         1531 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_7980_length_1531_cov_1.282520, whole genome shotgun sequence.
ACCESSION   DAWACS010000354 DAWACS010000000
VERSION     DAWACS010000354.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7980_length_1531_cov_1.282520"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    981..990
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          505 a          303 c          332 g          381 t
ORIGIN      
        1 agagttaaag tcactaataa aatatatttt atacttttga tatattactc ttaatatatt
       61 gatattacca atatttatct taactttaac ataaatcaga tttataatta tgcagagaat
      121 gaattttttt cagatacgga ggtaaactaa tgaaaaaact tactgcaaaa gcattagccg
      181 ttctaatggc gtgtacaatg attccggcaa cattaccgat tgtttcaaat gccgaagtca
      241 acgatgtcat agacggtaca agtgctgtac tatacagcca cgatgcttct gacagaccta
      301 tggaatcatt aaacagaggt cttgtcgttc aagcattgaa tggcgataac tacttatctt
      361 ggcgacttat ggttgacgag gacgaagtgt acggaacttc agaaagcaat gtaccattca
      421 atatataaaa acggtcaatt tcttgcaacc gaaacatatt cgacaaacta tatcgatcct
      481 aacggtacaa gtagcgacac atatcaggta gcgccgattg taaacggtgt tgagggagag
      541 aaatgtgatt ctgtcgctcc gtttgcaagc ggctcgaatt actttgatat tcccgttgac
      601 agaccgaagt caacgcttac cacaacaacc acaataacca ccgatgagaa cggaaacgaa
      661 ttacctgaaa atcagtggca cacggaaacc aaagtaaatg aatacactat cggtgatacc
      721 tcatgcggtg accttgacgg tgacggagaa tatgaactta tagtaaaatg ggactgcgct
      781 tcgcgtgata attcacaagc aagtcttaca ggaaatgttt atcttgacgc atacaagctt
      841 aacggcaaaa aattatggcg tattgattta ggtaaaaata tccgtgcagg tgcgcactat
      901 acccaattcc ttgtttacga tttcgacatg gacggtaaag cggaagttgc ctgcaaaacg
      961 gctccaggtt caattgacgg nnnnnnnnnn tgcgggaaaa tacgtttccg aaacaagcag
     1021 cgttgaagaa atcagaaatg ccaacgacaa taccgtcagc tacgtaaatc aaaacggcta
     1081 catattggac ggtaacgaat attttaccgc atttgacggc gaaacaggta aaacaatcga
     1141 tacaatatac taccctatcc cacgtcttga ctacgaaagt tggggtgata caaacggaaa
     1201 ccgctgtgac agatacgttg caagcgtcgc atggcttgac ggtcaacgtc cttatgcggt
     1261 ttactggaga ggttactaca tgggcagaaa cggcagacag cgtcacgggg catgcggtat
     1321 cagtcttgaa aacggcgttc taaaccctaa atacaaattc gatacataca gcgaggatac
     1381 cgacgcatac actcctggta atgaaaaata tgtcggcgag ggtaaccaca atatgacggt
     1441 tgcggacgtt gacgacgacg gtaatgatga atttataagt gcaacgcttt gctatgaggt
     1501 aaacgacgag gacaagctta tgccgaagtg g
//
LOCUS       DAWACS010000355         1522 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_8031_length_1522_cov_1.869802, whole genome shotgun sequence.
ACCESSION   DAWACS010000355 DAWACS010000000
VERSION     DAWACS010000355.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8031_length_1522_cov_1.869802"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          492 a          311 c          296 g          423 t
ORIGIN      
        1 atactaatat tacaccaaat cacaaaatat tcttgtctat tttataccaa tccttttttc
       61 gtttttgtgc tacttctaca atttttcttc gcttgcaaaa aatatattgg cgcattgcac
      121 aaagccgtgt tattcttagc tcatgtggag tttaaggaaa tgggagagca tgaaacgaaa
      181 ggagagtttt atgaaaaacg aatcacaact aaagagttca aagaggggcg tactgttaag
      241 acttgccata tttatggctt tgatgatttc cgcattcatc ataccaagtt cagctttggc
      301 tgatttcggt tacaccgagg acagtacaaa ttacaccata gacacgggtg caaaccttgt
      361 gtttaaagta aaacgttcaa acggtgatat ttcatcgctt atttacaacg gaactgacta
      421 caacggctac acaaacaaaa attcacacgt tgaaaccggt cttggtcagt cggacgtaac
      481 aatttcacag ccaagcagtt cagtaataat ggtcaaagta gtttacggaa cattagagca
      541 atactacgtt gcacgcaaag gcgaaaacaa catctatatg tttacatata tcgccgatga
      601 cagtgtaacc gtaacacgct acattgtcag acttaaaccg tcactattcc ctgttttgaa
      661 tacttctaac agttggtata gttcgtattc aacccttgag gcaaaggata tatttaccga
      721 cacctcaacc ggttacacat attcaaaaca ttattcagat acaagagtaa tggattataa
      781 ttacacaggt atatcaaacg gcaacgtcgg tgcatacatc gtacgcagta atcacgaaaa
      841 agcatccggc ggtccgttct atcgttctct tatcagagat aatacaaatg ttgccgtaaa
      901 tctttacgaa atactttact acggtatggc acagaccgat gttaagcgtt acggcttgca
      961 aggtccttat gtacttgcat tcactgacgg cggcgaacct tcatcaaaat tatatgccgg
     1021 caatttaaag acagattgga ttgattcgct tggcattcac ggttgggtcg gttcaagcgg
     1081 cagaggcaga gttgccggag taggtatcaa aaatatgaaa tcagattatg agtacgttgt
     1141 aggtttctca aatgacgagg ctcaatattg gacaaaagca tcttattcaa atggatactt
     1201 ctcctgcaca aatatgctgc cgggaacata cacaatgaca atttacaaaa acgagcttgc
     1261 tgtttacaca actgatgtaa cggtaactgc aggcggtaca aaaattttaa attcaataac
     1321 aattaccgat gacccgtcag acaatgacgt aacatggaga atcggcgatt gggacggtac
     1381 accgcttgaa tttaagaacg gttcacttat gactaaaatg cacccggcag acagtcgcgt
     1441 tgaatcttgg aaaggcaact acattgtcgg cacatcagac gcatcatcat tcccttgtta
     1501 catctttaaa gatgtcaata ac
//
LOCUS       DAWACS010000356         1519 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_8044_length_1519_cov_1.684426, whole genome shotgun sequence.
ACCESSION   DAWACS010000356 DAWACS010000000
VERSION     DAWACS010000356.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8044_length_1519_cov_1.684426"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    643..652
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          475 a          244 c          330 g          460 t
ORIGIN      
        1 tagtttttcc gcgacggcag ataaagcctg tttcgttgtt gccgcagtcg cgagtaccgt
       61 ttgtaaatat atgaatattc tccggagtga aaccgcagtc acgcacaaga taaagtgctg
      121 acgcaaatgc ggaaagtcct ccgcctgtga tatatgcctg ctttttcgta agagtttttg
      181 tgtgttctgc aagcattgtt ttgtgttttt tgtgctttga aataacaatc gcagtagtgg
      241 cggcagccgc tgttacagcc gcaccgattg caataagttt tgtaagatcg ctttttttca
      301 taataacacg ctcctaaaaa tcgttcaatc aattctattt acttattata tcacaaaaaa
      361 caaattttgt acagtaaata ttaccctgca aaaagtgaac atactgtaaa cattttgatt
      421 gaaaaaaatg tcggattatg atataataat ctatactatc gttaaaggag aatatattat
      481 gaagaggatc ggaaaaaaag gtaggtttgt actgctgctt gttcttcaaa ttctggttgt
      541 tttaacaatg gtagcggaaa tattcaacag aaatttctat aatgtatttc tgtgtctgct
      601 aactttggtt ttatttaaca ttccgagatt tgtagactta aannnnnnnn nngataccgt
      661 actccataca attaacggat ttataatggc ggcgataggc tttgcactta ttgatatttt
      721 aaatcaagac ccgagatttc atattaatat gtcaccgttt tttgtagcgt tcgtggcatt
      781 ttgtttttct atgacggtcg gagttgtttg ggagttcttt gagtttggta tggaccaaat
      841 tttcggaatg gatatgcaga aagattttat cgtgaatgca atacattccg caacgcttaa
      901 cccgaacgga cttaacggca cggtcagctt aaacggcata acaagtacga ttgtgaacta
      961 taacggagga cagtacgtta tagagggagg gtatcttgat gtcggaataa tcgatacaat
     1021 gaaagacctt atggtgaact gtatcggtgc ggttgtattt tcgattatcg gaatacttta
     1081 cataaagcac agaggtaaag gaaagattgc gaaaagtttc attccgctta tgaaaacaac
     1141 agaggaaatc gaagaaactc aaaaaatgat tgaagaattt aagagtaaga caaggagaaa
     1201 taagaatgag aaagttaagc ttaatattgg cgtcaataat gacggtgaca atggcattgg
     1261 ttccgacagt acaggcgagt gatattacgg taaaggtaaa cggtgaggaa attcatcccg
     1321 aaatggcgcc tgtaattgtt gaagaaagaa cacttgtacc tcttagagcc gtttcggaga
     1381 cacttggctg tgacgtgtcg tgggacgcag atacaaaggg cataacactt tgcgacggaa
     1441 gtaatctgta ttttacttgg attgacaaag atcacgcatt taaaacttcg gcaactgcac
     1501 ttgaagatac aactgtaat
//
LOCUS       DAWACS010000357         1515 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_8065_length_1515_cov_1.465753, whole genome shotgun sequence.
ACCESSION   DAWACS010000357 DAWACS010000000
VERSION     DAWACS010000357.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8065_length_1515_cov_1.465753"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    267..276
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1283..1292
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          515 a          298 c          246 g          436 t
ORIGIN      
        1 gcttattaca gtgaatttta cacaacaaac tttacagtta attcacttat taaggatatg
       61 aaaagttcta tagattttat atcaaattta tctgagaatt aaacacaaaa ggcagccgac
      121 attatagtca gctacctttt aatattctct gaatcccaca aattcaaata caacttgttt
      181 actgtatttc ccatcagtaa ttatttccgt aacatttctt tcactgcacg cagtaacaac
      241 ttttatatca cgttccttca gcaagtnnnn nnnnnngtta attactgtat atgcaagtga
      301 aaattcaccc tggcataaaa cacacttcgg atttttttct aaaatatcac caacacattt
      361 ttttgccata gtcgatattt ggctttttgt ataagttggc ggaacattcg gaaacggtat
      421 atctaatatt ttgccatact gttgtgcctg ttctgtctgc tctttactcc aatttttact
      481 cggatgattt gaaaaattta taaacattta atgtcttcct ttataattct tattacctct
      541 attttatcac acttacaaac aaatttctat atttatgtaa aatttttatt tagcaaaatg
      601 tataatacag aaaattaagg aaacaatact aaatactaca atgtatatgg aggaacacaa
      661 aatgaatcaa caacaaaaaa aggaaacaag aaagctacca agcttttaca tagcactttg
      721 ttgctgtgta ttggcaatcg gtatagccgg ttattttacc gaaagacaca attcagactc
      781 atcaagtatg ctaagcaatg agatagccga taacagtggc agcgaagttt tttcggattc
      841 aaccgacaaa ttcacatctt ccgctctgaa cgtttcagaa gatgaaactc aagaggcgtc
      901 atcagcaaat gtagaagaga gttcggaagt tgtatctgat accgaaaccg ctcaaagtac
      961 cgacactcaa accgatatac ctgttatgtc cgagcaacaa gtacctgtcg aggactatgc
     1021 agtcaacaat cccgacgtcg accaaaccgc agttatcgta tcatccgaac aacctgcatt
     1081 tattatgcct gtaaacggaa atacacttga aggatattcg gacaaattaa aatacaacga
     1141 acagctttcc gattggcgta cccacaacgg cattgacatt gccgtcaaca caggttgcag
     1201 tgtacaagcc gtcgctgacg gcgttattga cgaaacaggc aaagacgcaa tgggcgaata
     1261 tgtaataata tctcacgccg cannnnnnnn nnttataaca aaatatatgg gacttgaaaa
     1321 cattgaaaat cttactgtag gtaaagaaat aaaatcaggc gaagttatcg gaacgtcagg
     1381 taaatgtacc ggtgaaaatg tgaccgatcc gcatattcac tttgaaatgt caaaagacgg
     1441 agttttggta aacccaaccg actaccttcc tcaacaataa aagcaaaatg agcctgtcga
     1501 cattttgtcg acagg
//
LOCUS       DAWACS010000358         1511 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_8087_length_1511_cov_2.385989, whole genome shotgun sequence.
ACCESSION   DAWACS010000358 DAWACS010000000
VERSION     DAWACS010000358.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8087_length_1511_cov_2.385989"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          414 a          373 c          250 g          474 t
ORIGIN      
        1 taatgtcata tgaaagaacg ctgtgatgag caccgccggc tttaatccaa cattcagccg
       61 aaacagcaag tgacggaagc ggtttccaca ttacacccgc aacaggcaat ttaggcattg
      121 gctttaacgg attacaagcc ttaatatcat ttacgattaa acgcattctg ccgcccatat
      181 caacaagcga agcaacgatt gcgtcaccgt tctttgagtc aaatacaagt cttgcaggat
      241 cgctcttacc cccgatacca agaggatgta cctgaatttt cggcttatct gccgcaacaa
      301 gcgggcaaac ttcaagcata tgcgcaccca aaatatgttc attacctttt tcaagatggt
      361 atgtatagtc ctccataaat gcagtaccgc ctgtcatgcc ctcagccatc gacttcatta
      421 taacgtccat agcggcaact ttccagtcac cctcggcacc gaaaccgtaa ccgtctgcca
      481 tcatattctg tgcagcaaga cccggaagtt ggtcaagacc gtgcaatgta tcaaaatgag
      541 taacgaatgc actgtaatca aactgttcac acatttttct tatagcgact tcttctttcg
      601 cctgataacg aacagcgtca atattatccg tatccattgt ataacggctt tcatattcag
      661 ccatttttgc atcgatttca gcctctgtaa cttcattcaa aatgtcaaca atatcgccga
      721 ttggtctgta ttcaacgtcc caaccgaact tgatttcagc ctcaacctta tcaccctctg
      781 taacggcaac ttggcgcata ttatcgctga aacgtaccat tttaagctct tggctgattg
      841 cataaccgca agcacttctc atccaatttg caagctttgt ttgaggttct tcgtcctccc
      901 aataacctac gataaccttt ctcgccattc taagtcttga accgataaat ccgtgttctc
      961 tgtcaccgtg tgcagactgg ttaaggttca taaagtccat atcgatttca ttccaaggaa
     1021 tttcacgatt aaactgtgta tggaaatgac agtaaggctt tttgaagtcc tttagaccgt
     1081 ttatccacat ctttgacgga ctgaatgtgt gcatccaagc aattacaccg gcacatttat
     1141 catcatatga tgcctctttg aaaacttttt taatttcagc cggagttttc actgtcggct
     1201 tgtacacaat cttacaaggc attttgcctg acgcgttaag accgtccacc atagcctgcg
     1261 aatcctttgc aacctgttcc aaagtttcag ggccgtaaag atgttgactt cctgttacaa
     1321 accaaaattc atattcttta attgatttca ttggtttctc tctccttaaa tacataatac
     1381 tctgttttgt gtgtaaattt cacactatat atttattata tattaatcgg tttatttttt
     1441 caataggttt ttgtatgttt cgtccaaata ttttttcaca ttatcgcact tcataaaccc
     1501 cgacattata c
//
LOCUS       DAWACS010000359         1509 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_8110_length_1509_cov_1.790234, whole genome shotgun sequence.
ACCESSION   DAWACS010000359 DAWACS010000000
VERSION     DAWACS010000359.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8110_length_1509_cov_1.790234"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    269..278
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          395 a          378 c          226 g          500 t
ORIGIN      
        1 gccgagtgca agagcggatt tatcgcattt tccactttcc gccatgcgtg acgcttttgt
       61 ttcaacaccc gaaaaattca tatacgcacc gtcaacggct atcggaagtt tttcactttt
      121 ttcagccttt agagcaagct gttccaaagc cttaccggca ggaaaatgca agcccatttt
      181 tacgcccact ctgtcaataa attgtccggc acttatatcc ttacttccgc cgacaatttc
      241 attgtcaaaa ttaaatccgt tgtaacggnn nnnnnnnnat atattcccgc cattatgtgt
      301 ccgtcttgat gcgaaaattc atacaaaggc acacccaaag tatcagcgac aacctttgca
      361 tacccctcac ctgcaaggaa tacgggcata tatgatccct ctacacatct cggtcttgta
      421 ctgactccaa ccgccttaac atctttaaaa tcaatctgct ccgacaattt ttcatacatc
      481 accggcatta atttcatatg ctgaaaaacg ccgtcacttt gacgtattcc tctttcaccg
      541 tcttttacgt ataaaatctg tcgtacattt atatcggttt ttccgtcagt ccacgcagtt
      601 gacgttgtgt aattgcttgt atcaaagcct attgtactca cgctaactcc ttaaatacat
      661 tacccaaaag tccgtttacg aatgtagggt caccgtcatc gccgtaacgc tttgcaagtt
      721 caaccgcctc atttatcgca actttcggcg gcacgtcgtc catatacttc atttcaaata
      781 ttgcaagctt cattattgaa agtgatgctt ttgaaattct tgaaagcgtc caacctgctt
      841 tcaaatactt tgcaaccgta tcttcaagct cgctttcgtg ttccttcact ccgtcaataa
      901 tctgtcttat ataaccgaga tttgtttcac attcgggacg cgcatttaaa agctcctcca
      961 aaattacgtc catatcatcc tcgtgcatat tcatttggaa cacttgtgta aaagcagcgt
     1021 cccttgcttg acttctcgtc tgttttttca tatttttatt cctttcccaa aataataagg
     1081 ctacttaaat tctatgttaa aactatacca caataacata aaaaaatcaa ggcttatgcc
     1141 tataagacac aatccttgat ttattttctc agtcttgaat atcctcatcg tccatagaat
     1201 cttcatcagt gtcaacagtc ggtaaaggtg tttcgtttac gttgctgtca agttcgattt
     1261 tttcttcttt ttccttttca aagcttacgc cttcgatatg gatattaacc ttttgaacat
     1321 caagtcctgt cattgtttca acgctgtttt taacgttttc ttgaatttcc caagaaagtt
     1381 ccggtattct tacaccgtaa tccaccacaa tataaaggtc aatcgttgca gttgtttcgt
     1441 tcatatcaac cttaacgccc tttgacggat ttttctttgc accgaacatt tcggcaatac
     1501 cgccggcaa
//
LOCUS       DAWACS010000360         1507 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_8120_length_1507_cov_2.125344, whole genome shotgun sequence.
ACCESSION   DAWACS010000360 DAWACS010000000
VERSION     DAWACS010000360.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8120_length_1507_cov_2.125344"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    574..583
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          537 a          222 c          350 g          388 t
ORIGIN      
        1 agtcagatgg tttttgacct tgaacagagt gttgacctta caaaaattga aaaagaggca
       61 acaacaagat tgggtatgca aagacctgaa aagtatcaga caatatatgt aaacgtaaag
      121 caagatgata tgacagagaa aactgccggt gaagtagagg gttttggaca cggtattgtg
      181 tctgtattga aaaagattgg tggtaatatt gtacaatttt tcagcataaa ataaaaatgg
      241 acttctataa ttataaaatt atgaaaaatg tacatttatt aaagtattaa aaggaattga
      301 taaattaatg aaaccatcat taaatgaaat taaaaagaaa acgatattta tggtgtgtat
      361 cgttgctgta ctgcttgcgt tgcttgtttt gcgagtggca tattggcaga taataagagg
      421 tgacgagctt tcgagcaagg caaaggcaca acagcagggc agcagtattg ttacagcctc
      481 aagaggagat atttacgacc gtaacggtaa ggtgctggcg gaaagtgcaa cggtaaacac
      541 acttatttgc aatcctaagg atattgaggc ggannnnnnn nnnaattcaa gataccaagt
      601 tataaaaaaa cgtcttacga acgaacagac cgaggcggta aagaaattaa gagattcaga
      661 tgttgacgca acgattgcaa aagcatttaa aggggtatat tttgaaaatg attcaaagag
      721 atattatccg ttcaacgttg caccgcatat tttgggattt acgggatatg acaataacgg
      781 tttgcaggga attgaactta ctttcgacga tgagcttatg gggcgtaacg gaagtataga
      841 aactaatcag tcggcaaagg gtacaacact taaagaccaa caaaatgagt atttgaacag
      901 ttctcaaaac ggtgatgacg ttgtgcttac gattgatgaa acaatacagc atttccttga
      961 aaagcacctt gaagaagcgg ttacggaaaa caagcttaaa gagggtgcgg caggtattat
     1021 tatgaacccg aaaacgggcg aaatacttgc aatggcaact aagcctgatt ttgacgtaaa
     1081 taatccgtat gatattgacc agtttgaaaa atatgcgata gatttgggat atgatacatc
     1141 agatgacaca ggtgacgatc ccgatgcaac accgaaaccg acagaagatc cgaataattt
     1201 aagtgacgag gccattgcga aaataagaaa taaaatgtgg agaaacaagg ttatttcgga
     1261 tacatatgaa ccgggctcga catttaagat tattactgcg gcggcggctt tggaagaaca
     1321 cgttgtagac cttaattcaa cattctattg tccgggattt aagattgttg ccgacagaaa
     1381 aatcggttgt gcaaacaaga gcggtcacgg cgctcaaaca tttgtagagg gtgtaagaaa
     1441 ctcttgtaac cctgtattta tggaattggg cgaaagactt ggatatgaga aatttatgga
     1501 atatttc
//
LOCUS       DAWACS010000361         1500 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            NODE_8171_length_1500_cov_1.606228, whole genome shotgun sequence.
ACCESSION   DAWACS010000361 DAWACS010000000
VERSION     DAWACS010000361.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8171_length_1500_cov_1.606228"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          491 a          279 c          325 g          405 t
ORIGIN      
        1 ttgttatact ttgctgtgac ggtaatattt tcggcggaag caataacaac tgctgttgcg
       61 atccatgctg cgacccttgc tgtgacaatc acaaaaacga ttgctgctaa aatctagtgt
      121 acttaaaggt gccggcatat cggcaccttt tttagtgcaa aataaatttt acaaaaaatt
      181 aataaaaaac agtagtaatc cttacgtttt tgtgatataa tatacgttaa taatgcgata
      241 attccattct ttgggattac acagaccgat attatttatg aggagaaaaa taaatgagta
      301 tattcgacaa aatattcggc acatattctg acagagaact aaagagaata cgtcctatcg
      361 cagacaaagt tatggcactt gaggacgata tggcaaaact ttcagatgca gaattaaaag
      421 ccaagacacc tgagtttaag gaaagactta aaaacggcga aacacttgat gatcttcttc
      481 ctgaggcatt tgcggtaatg cgtgaggcaa gctggcgtgt acttggaatg aaacacttct
      541 atgtacaggt tatcggtggt attattcttc atcagggacg tatcgcagaa atgaaaacag
      601 gtgaaggtaa aactcttgtt tctacacttc ctgcatattt gaacgcttta accggtgacg
      661 gtgtacatat tgtcaccgta aacgattatc ttgctaagcg tgacagtgag tggatgggaa
      721 aagtttaccg ttatcttggt atgagtgtcg gacttattat tcacgagctt gacaacagtg
      781 aaagacgtga ggcatatgcc gctgacatta cttacggtac aaataacgaa cttggtttcg
      841 actatcttcg tgacaacatg gttgttcata aagaagaaat ggtacagcgc ggtcataatt
      901 atgcagtagt cgatgaagtt gactcaattc ttatagatga ggcgagaact ccgcttatca
      961 tttccggtat gggcgaggac gcaaatgacc tttacagagt tgccgatatg ttcgttaaac
     1021 gtcttaaaaa ggttgtcgtt acagaacacg acgacaaaca gcttgacgaa gatgtagacg
     1081 cagattatat cgttgacgaa aaagcaaaaa cagctatgct taccgaacag ggtattaaaa
     1141 aagctgaaca aggtttcggt atcgaaaacc tttccgaccc tgaaaatatg aaacttcaac
     1201 accacatcaa ccaagccttg caggctaacg gtgttatgca ccgtgaccaa cagtacgttg
     1261 ttcaagacgg cgaggttatg attgttgatg aatttacagg acgtataatg ccgggcagac
     1321 gttacagtga cggtttgcac caagctatcg aagcaaagga aggcgttaaa atcgaaaacg
     1381 agtcaaagac tcttgcaaca atcacattcc aaaacttctt ccgtctttac aacaagcttt
     1441 cgggtatgac aggtacggca cttactgaag aagaagaatt ccaacatata tataagcttg
//
LOCUS       DAWACS010000362         1324 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            k99_8883_length_1324_cov_3.0000, whole genome shotgun sequence.
ACCESSION   DAWACS010000362 DAWACS010000000
VERSION     DAWACS010000362.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1324)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_8883_length_1324_cov_3.0000"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          459 a          259 c          299 g          307 t
ORIGIN      
        1 aaacgacggc ataacatgga cagatttgta tacaatcagt acggcgattc cgtcaggtca
       61 gtacacaaca ataagcagta cagatttggc tgatcaaaac gcatatcgtt acttcagata
      121 cactaacgac accaatatga caaatattgc agaatttttg attgacgcaa ctccgtcaga
      181 gtaaatggca gacggagctc cgagcgttac ggatattgat gaaatgagcg atgattttga
      241 aaatgcaaca aatatcttta atgcaagtgc aggagatttg tcggcagacg gaaatcaagt
      301 gtatgcaaca ggtcttgaaa gattcggtaa cgtatttgtt cctgttaaag caactgcaaa
      361 agcagaactt tcagaggcaa agtcacttac aagcaaggat aaattcagac ttacattcga
      421 tatgttctca ggttgggagc agaacggtaa ggaaaataca ttctctgtta aagacgcaga
      481 cggcaacgag gttgtaggat ttacaatcac aggcggcgga tataatctta atcaaatgcg
      541 tatcggcgga acagatgtac ttgccgatgt atcggaaaaa cctgtaatcc aatgtaaatc
      601 aactgatatc agaaacggtg catttcgagg tgcaaacggt tggcagactt catcacagaa
      661 gtacgcaaac aatacgggat ttaataaaaa ggtagaaatc ataatagacg gaacaggttc
      721 ggcaagcgta agctttactg gcggtgagac tgatgtaagc tacacaggaa caataagcac
      781 acctataagt gttaaatcgc ttgaattaac aggttcatac aacggtgcaa gaggaagagt
      841 tgtttcatat gataactttg acgcagatgt aatatcatac agcagtgagt tggcggcacc
      901 gacgccgact cctgaaccaa ccgcagcacc gacagttcct gaaagcggcg agcttatcaa
      961 tatgaatttt gataacggcg acttaacaag tacatcatca tacggcaagg caacaggtac
     1021 accgaaattt gttacggttg acaataagaa gtgtattcag tttgacggaa caagcggaac
     1081 ggttgttacg cttacagacg caaacggcaa ttcgcttttg acaggtcaga aaaatattac
     1141 gatttcattt aaggttaagc cgacaaccac aacaacatca tggtggttct ttgccgcacc
     1201 aaacggcagt gcacagacat atcaaaagga acagtatctt ggtgcaatga caaacaacgg
     1261 cacacttaca tccgaaagat ataataactc cggtacaaga agtcagtcgg caacaggtgc
     1321 atat
//
LOCUS       DAWACS010000363         1288 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            k99_19660_length_1288_cov_5.0000, whole genome shotgun sequence.
ACCESSION   DAWACS010000363 DAWACS010000000
VERSION     DAWACS010000363.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_19660_length_1288_cov_5.0000"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          369 a          286 c          214 g          419 t
ORIGIN      
        1 ctttataagc cttgccggca cttctcggag atgctgcaag cacttcgatt gtataccatg
       61 ggtggtcgtt aagaagtgta ataaaacgct gtccaaccat acctgttgca cctataatac
      121 ctactttgta attgtctttc attttgactc tcctttgttt tttataaaat aaaaagcagt
      181 agtcagaaac tactgctatg tttgcatata acaaaaacag cgatagctcc ccaccttacg
      241 atgacagtca taccgctgtt aacgatatgc ccagaatgaa tatccgcaat aatattcaaa
      301 cttcggcact gtgtcctttc cgtatacatc aaagataatt gcatatctcc gtatacatac
      361 tgatacacaa gcgcacctct accttgctta tatattatta tactaccact attaattgta
      421 tgtgtcaata ggaaaaatat tattttttta ttacattatt taaacgttgc aaattcctgt
      481 gcccaataaa taccgtatga tcctcctcgt gcaagtccta cgcctatata ttcaaaatcg
      541 ggatttaata tatttttgcg gtgtcccggt gaattcatcc aagaatccat aacattttgc
      601 ggtgaagact gacctgcggc tatattctcc cctgccgaag aatatgttat tcctgcggct
      661 ttcattctgt caaacggagt ttgtccgtca ggagtattgt gtgcaaagaa gtttctgtct
      721 atcatatctt cacagtgact ttcggcaaga gcggcaagcg aatcgtccca ttttaacggt
      781 gcaacattat attttgcacg ttcttcgttt acgagattca gaacttcata tccccaatca
      841 tcaaaatcac tttcattatc ggtttcaata taaactgtat gcgtgtcacc gtcccaaaat
      901 acctctgcac cggccgattc cgatacagcc cttaacggta caagggtgta atcattgtag
      961 attatagggg gcgtattaag ctcgacagac gtatcattga catacatcgt attttcgttt
     1021 ataaacaaag ttatggttaa gtcgtctttt tgagccgtta cacgctgttc gtcgccaaac
     1081 cactgtacaa ccatatcaag attttcaaat atcgctctga acggtacaag agttgaatcg
     1141 ttttgtatta ttggtgactg ctcaaatatt aattcatctt cgtttagata taccgttata
     1201 tcatcatcgg caaaaaccgt cggtgacata atcatcagtg ataaaaaaag tgctgctatt
     1261 tttttcatat catatccctc ctcaccgc
//
LOCUS       DAWACS010000364         1269 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            k99_28756_length_1269_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWACS010000364 DAWACS010000000
VERSION     DAWACS010000364.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_28756_length_1269_cov_4.0000"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          370 a          288 c          154 g          457 t
ORIGIN      
        1 cgtcggcata tttcatatta atcttgtttt tcttcttttt tcttttcttt gaaaaccatc
       61 ttaagcaaat caataccgat tatcatattt tcggtaaacg gattaattac aaccgcctca
      121 acaggattct tttcatcata cactactgta acgcattgct cgaagttaag tttcaataat
      181 acaccagacg gtgacttttg aaccatagat tcttcatcag taaatatagg cagaaatact
      241 ttcttatcac tacttgtcaa aaatgccgga ttaaatacag ttccctcttt caatcttaca
      301 ttaccgtttt caagtttttc aaacgggtct tccacattac atactgacgg aagcaaaaac
      361 tttctgcctg acagcatttt taatacagca atcaaattct gttgatttct ttcttgttta
      421 aatgctttca cctttgattt aaaaacttca ttttcaaaag ctatgtcgtc tttaggtgct
      481 tgttgcggtt gctcttcagc tttttcttct ttgttgccgc tcattatttc atcataagcc
      541 ttggatattt cgtcctttag ttcattatcc tcttgagttg aaactttttt cgtttcttct
      601 tttttgttct ttttaaatat tgccattttt caatcctcac tatttatcat attacttcta
      661 ttatacatat taacaaatag tttgtcaatg acaaaaagtt ttacgcctcc ggcattacta
      721 ttgcggcaat aatataggca agcacacctg taccgaatgc aagacacgat aacaatacca
      781 cacccaatct tactaaagta gaatcaatat tgaaatattc ggcaataccg ccgcacacgc
      841 cgcatatttt tctgtcagtt gaacttctaa acagtttctt cattataatc accctccata
      901 ttatctattc cgaattcttt aaagaaatca tttatatcat cattataatc atcatacata
      961 ttattcggct ctattcttgt ttcgacgtta attccaattc cgcgaagtgc tttttcaaca
     1021 tcagtgccca tcacaacacc gcttgcacac aacgcaattc cgagtgcgac cgacacagca
     1081 cagataataa gtacaacttt tgttatagtc tttctcattt tgtcctcctt atccatcacg
     1141 cttttttatt aaaaagtctt gagcctaaag caacgatttt tctgattatc cacggaaaca
     1201 gttttatgca tacaaccaca gtcgcccaag ctataaacaa tcccaagccg aagaatatac
     1261 atccaacac
//
LOCUS       DAWACS010000365         1229 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            k99_719_length_1229_cov_3.0000, whole genome shotgun sequence.
ACCESSION   DAWACS010000365 DAWACS010000000
VERSION     DAWACS010000365.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_719_length_1229_cov_3.0000"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          311 a          291 c          186 g          441 t
ORIGIN      
        1 atgtgaacaa gaccgtcaac accgccgatg tcaacaaatg caccgaatga tgtcagtgcc
       61 ttaacagtac cttgatactt cttgccaact tcagcctctg cccagaactt ttcttctttt
      121 tcttttcttg cagcctcaag aataactctg actgaaccga taacacgtct tcttctttcg
      181 tcgatttcga ttatcttata gtttacagtt gttcctacaa ggctttgaag gtcctgtaca
      241 aatcttccgg atgcctgacg tgcagggata aatacctgag aaccgtctgt aagtgcaata
      301 acaccgccct taacagcctt tataatcttt ccttccaaag tctcgcctga ttcataagct
      361 tctttgattt tgttccagct ttccatagct accaatttct ttcttgatag aactacttta
      421 ccctcagcat cgtttacgcc tacaacgtaa accttgattg tatcgccttc ttttactaca
      481 tcagacggct taagtgcagg gtcatcagta agctgatcag ctgctaattg tccgttatac
      541 ttaaagctac ccaaatcaac gatgacctca ttattgcgaa cttctacaac tgttccctct
      601 acaatgtccc cgttattaag agttttactt tcatactgtg caaacagttc tgcaaagttc
      661 tcctcatttt ttaaattttc actcatagcc tttactacct cctcgataat actgccgggt
      721 gtagacgctc ctgcagtaat acctatttta ttatatgttt tttcttgagg taaatcctca
      781 aaagtttcga tgtgataggt gtgtttacag tttcgctttg atatttcaaa cagctttctt
      841 gtatttgagc tttcctttcc cccgattaca atcatcatgt ccgaatttct cgaaagtttg
      901 tctgcctctt cttgcctatc cttcgtcgca ctacatattg tatcaaaaac tagggtactt
      961 ttgcaagttt tttttacatt ttgtacgatt tcaccaaaaa aatctctatt aattgtggtt
     1021 tgtgctacaa tacatatgtc ttttgaggct aaattaccgt caatttcact tttcacgtcg
     1081 ttaattatga cagctttgtt atcgcaccag ccatttattc caataacttc cggatggttt
     1141 ttatcgccga caataataat ttgatacccc tctttgtgat gtttcgatac aattttatgt
     1201 atctttgcca caaaaggaca ggtaaggtc
//
LOCUS       DAWACS010000366         1169 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            k99_5249_length_1169_cov_3.0000, whole genome shotgun sequence.
ACCESSION   DAWACS010000366 DAWACS010000000
VERSION     DAWACS010000366.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_5249_length_1169_cov_3.0000"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          408 a          204 c          270 g          287 t
ORIGIN      
        1 aagtatcgca gacagtgctt gcaactttcg gaacgggcgg taataatcag ccgtttgtcg
       61 tgcatacacc aaaaacttta aaaatcagat gcggatgtga cggcggtggt aaaggtgcat
      121 tgatacaaga aaataagtcg gcaacattaa gctgtaataa tgaccagacc ttatttgaac
      181 cgaaagcata tgggatatgc tcaaacgaca gcaattcgat gaagtctgac aatccgaaca
      241 gcggaatata tgcggcagat acttctcgta ccattgactg cggaggtgta aatccgtcat
      301 ctaatcaagg aggtatggct gttgttgcat tgcagggttc aataattgga cgtaaggaga
      361 aaaacggacc gaacggcagc ggatttaatc aggatacatc atttacatta aatacagttg
      421 accgacatgc agttgcatac ggaattgacc gtgctacatt taatcaagga caaaatgcgt
      481 tatatgattt tgcaatagaa aaagagaaac agccgacaat ggtggcaaaa ggtccgggag
      541 cggtagccga acctgcatat tcggcaagca aggcatcatt ctttacaagt gccgaaaaag
      601 aatgtgcaaa tacacttgtt gcaagcgatt acaaagaccc tccgcttgta aatgatacaa
      661 acggtacgga atatatagta agacgtctga cacctaaaga atgtgctctg ctgcaagggt
      721 tccctgtatg gtggtgtgac ggtttggaaa cagaaaatcc tacggaagaa gaaattcaga
      781 aatggtcgga tatttttgaa aatcacagaa aagcactttg taaaagtaca aaaccgaaaa
      841 caagaaatca gattataaag tggcttaaaa atcctcattc cgacagtgcg gaatatacga
      901 tgtggggaaa tggtgttgca cttccgtgcg tgttctatgt attgaacgga attgctcact
      961 atgctgaact cacaaattct gtaacgtaat attgtgtact atacaacttg atatattttc
     1021 tgcatgacgg taatatgtgc ttaacaaaaa aataaaggag gattgccgta atggaaatta
     1081 aatataatct gacaggaacg gaaagaaagg ctctcgtgaa aacagttagc aatataatcg
     1141 gagaaaagtt taaatatctt ggagcaccg
//
LOCUS       DAWACS010000367         1082 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            k99_15960_length_1082_cov_3.0000, whole genome shotgun sequence.
ACCESSION   DAWACS010000367 DAWACS010000000
VERSION     DAWACS010000367.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_15960_length_1082_cov_3.0000"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          375 a          169 c          253 g          285 t
ORIGIN      
        1 aaatagcttg cttttgttgc ttgagaatgt gaggttgtga aaatatgaat ttgggatttt
       61 atatgcctac aaaggttatt gtgggaaaga attgtgtcag agaaaacagc gatgttttca
      121 aaataggaaa gaaagcactt atcgtaacag gcaaaaattc cgcaaaatta agcggcgcac
      181 ttgacgatgt caaatatgca cttgaaaaga ataatattga atatgtaata ttcgataagg
      241 ttatgagtaa tccgacagtt gagtgtgtgt atgacggtgc ggaaacggca atggaggaag
      301 aagttgattt tgttattgcc ataggcggcg gctcgccgat ggactcggca aaggccattg
      361 cattgctggc agtatcggac attgaaagaa agaatttgtt tgacggcaac ataggcaata
      421 aggcacttcc tatgatacat attccgacaa ctgccggaac cggttcggag gttacaccgt
      481 atgcgatttt gacaaaccac gaaaaacaga caaaaaagag tattgcgtca ccatgccttt
      541 tcccaacata cgcattgctt gacgcaaaat acacaaaaaa tctcggactt gccactacgg
      601 tcaatatcgc aatagattca ctttcacatt cggttgaagg aatgatgtca aagagggcga
      661 atataatggc tgatgtaatc gctaaagagg ctgttaaaat tattgcgtca acatttgacg
      721 atttgattag tggtgaactt acggaagacg acagagaaaa actgctttat gcgtcaatgc
      781 ttggaggtgt tgttatatcg caaacgaaaa caacagcggt acacgcaatg ggatattcgc
      841 ttacatattt caaaaatatc gaccacggca gagcaaacgg attgttgctc ggtgagttta
      901 caaagtatat tgaaagaaat gataaggaca gagttaagga aatattgtct tgtatgaatt
      961 tagacagtgc agaagaattt aaaaatattc ttgcgaaaat tttgggtgat aaagaaaagt
     1021 ttgaaaaaga agaacttgaa ttgtatgcag aaattgctca aaatgcaggc ggtacactaa
     1081 ac
//
LOCUS       DAWACS010000368         1059 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            k99_11249_length_1059_cov_3.0000, whole genome shotgun sequence.
ACCESSION   DAWACS010000368 DAWACS010000000
VERSION     DAWACS010000368.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_11249_length_1059_cov_3.0000"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          351 a          158 c          252 g          298 t
ORIGIN      
        1 gcacttggca caaagtggga gattgaggac aggaacgagt cgaagtcaga aatatcacac
       61 aaattgagca tatacaaaaa tgcggttact caaaaaggta tggatacata taaatctttg
      121 caggagcaaa tgaaaaagat aaaacgtgaa gaggaaatgt acaaattaca aaaaatgcat
      181 accgcaacgc tgacaagtct tgaaaacagt tatgacaccg ttgaggcaaa taagaaatat
      241 cttcttgcga ctatagaaaa aaatgggatg aatatagaaa aaatcgtgaa aagcgtgaat
      301 tttgacgtaa agaatatgca aagtgtgatt acatcgttgt tcaatcagac tataagtgca
      361 ataaagtcga ttaaggtatc gtcaaattct tattctgaca acagaaatat cagcataaga
      421 ggggacagcc gtgatatttt gaacgcactt caaaacagag tcggactggc tgtatcatac
      481 ggtaattaca attaaagggg gattatatga ggaatggttt tatatttaaa gaccgtcatt
      541 cttccgattt cgatgtgacg gtgaggacta aatcaagacc gatattgccg agcgtaaaag
      601 agaatattgt cgatttgccg taccgtgacg gcggatatga tttttcaaaa gctaatcctt
      661 tcggacgtga gttttataat gacaggatat tcacggttac gtttaacata tatgccgata
      721 atcttgcgga aatgcagagg aagctgtctt tgctttctct gtggctttgc ggtgagggtg
      781 agctgatttt tgatgatatg ccattttcaa aatggcgggg gaaagtgtcg gacgaggtta
      841 tatatatgcc tgaacacagc ggtcgtaagg cggttatgga ggtgtcgttc agggcagaac
      901 cgttttgtaa atgtattttt gatacggaag gcccggcttt ggaaatgcct tttatgttag
      961 atgcaaatat acctattgat atttcgtgtt tgtttaaatt taatatttcc ggcagcggtg
     1021 atataagtgt atacaatttc ggtgacagac ctgtaagac
//
LOCUS       DAWACS010000369         1057 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            k99_28390_length_1057_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWACS010000369 DAWACS010000000
VERSION     DAWACS010000369.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_28390_length_1057_cov_4.0000"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          338 a          208 c          259 g          252 t
ORIGIN      
        1 cctaaaggac agcgaaggaa acgtacttga cgttcaaaag acaacaacag gttttcgtaa
       61 ggttgaatat gatataaacg acggcggtct gaagattaac gaccagcctg tatggcttac
      121 aggttacgca cagcgttcaa caaatgagtg ggctgtaatc ggtgtggcaa acgattggtt
      181 gcaagacctt gatatgcaat ggataaagga atcaaattca aactttatcc gttggatgca
      241 cgttgcgccg aagccgtcac aaattcgttc gggtgataag tacggtgtag tatcggcgtg
      301 tccggctggt gataaagagg ctgacgttga cggaagagca tgggatcagc gtgttgaggc
      361 aatgcgtgac gcaatgattt atttcaaaaa ctcgccatcg gtaattttct gggaagcagg
      421 taacaacgca gttacagcgg cacatcaaca ggaaatgaca gacctaaaga atactcttga
      481 cactaacggt gaaagattct gcggttgccg tacaatttca tcaacagacc aaattcaagc
      541 ggctgattat gtcggcacaa tgcttaacag acatgcaagc aatgcaaagg catcaatggc
      601 ggctataaag aagtatgttc ctattatgga aacagaatat gcccgtgaag aggctccgag
      661 acgtgtatgg gatgacttct caccacctga ctatgattac agaaataagt ggcttggtgc
      721 aggtgcgtca aagacggacg gttacgatat acacgacctt acatcggaag atttttcatt
      781 ggtagacgca agagcttaca atgaatttta ttcagaccgt gtaggcggtt tatcaggtaa
      841 tgactactat tcggctgcgg ctatgatggt atggtcagac tcaaatatgc acaacagaaa
      901 cacaggtact gaaaactgcc gtacatcagg taaggttgac cctgtaagaa taaagaaaga
      961 cgtattctat acaatgcaga caattcagtc aacgacacct aaattgcata tcgtaggtca
     1021 ctggaattat ccgcaactta gcgacgatac atataac
//
LOCUS       DAWACS010000370         1039 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            k99_9592_length_1039_cov_5.0000, whole genome shotgun sequence.
ACCESSION   DAWACS010000370 DAWACS010000000
VERSION     DAWACS010000370.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_9592_length_1039_cov_5.0000"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          248 a          267 c          176 g          348 t
ORIGIN      
        1 aaatatcaaa ttctttgccc aatctgattg cactgcaaat atcgtctgcc ctgtgtgcgt
       61 gaattttaac aggtatctgt tttttgagta caggtatcat tgcgtcaagc tttatatcga
      121 attcaggctt gtcgtgttct tcgctgtttt cattatatgc gtcaagctga ttcatataat
      181 ctcttgattt tataagcaat tcgcgtatca aagccattgt tcccatacgc gtaacaggtg
      241 cctcgtcctt ttcattgtac acgcattttg ggttttcacc aagtgcaaat ttcattgcaa
      301 caggtgtttt aataatcata tcatctacgc atattccgta cgtcttaacc gccgcaaact
      361 gtccgcccac agggtttgca ctgcccggtc ctgttacaac ggttgtaaca cctgcgttat
      421 acgcgtcttt aaacccacgg tcaaacggat ttattccgtc aatcgcacga aggtgcggca
      481 ttatcgggtc gctgtcctcg ttaccgtcat caccctcaaa tccaagcgaa tcttcaaaca
      541 ttccgacatg acaatgtgcg tcaatcagtc cgggcataac gattttaccc tctgcgtcaa
      601 taatcttatc cgcctcaaat actttatctt cacctacata ctgtatcttt tcatcaaaca
      661 atatacagcc gttcttgtac ttttcgcctg tcattgttac tatttcggca ttttttataa
      721 gtgtcttcat agttttatcc ttttaccttt tatatgcttt taattccgag ttcattcaca
      781 taattaaccg cacttgtcgc agcgattgca ccgtctgccg ccgctgttat aacctgtctt
      841 aacggtgata ctcttacgtc acctgccgca aatatacctg ttatgttagt cgcaaggtac
      901 atatccgtct tgacaaatcc gtgctgacaa cattcaaccc cgcaatcttt tgcaagatcg
      961 gacgacggct ttacgcctat tgcgacgata gcgcccgata cgttcaaaat actcttttcg
     1021 cctgttaccg tgtttctaa
//
LOCUS       DAWACS010000371         1038 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            k99_22724_length_1038_cov_3.0000, whole genome shotgun sequence.
ACCESSION   DAWACS010000371 DAWACS010000000
VERSION     DAWACS010000371.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_22724_length_1038_cov_3.0000"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          361 a          185 c          229 g          263 t
ORIGIN      
        1 atctaatatc tattataaaa tatctaagta caaaagtaaa gcaaaacagt tagaaaattt
       61 acaactgttt tagaaatctt ataattgtaa tgccctatgt gggagtgcta taatataatt
      121 aacggataat gtaatcttaa ggaggagaga aaaccaatga ataagaaaaa gattataagc
      181 acaatcttgg cgtgtgctat gcttcctttt ggcggcttaa tcagtgcatc tgctcaagat
      241 acaaaaccga catatgtaca gcttaatccg gctgacgcgt caccttttaa caacggtgag
      301 ttccaaggct ggggtactgc actttgctgg tgggcaaaca gattaggtta cagtgaaaaa
      361 cttaccaatg cggcggcaga ggcatttttc tcagacgaag gattgggact tgacatcgca
      421 cgttacaact taggcggcgg tgacgatccg acacataatc acatcaaccg ttctgactca
      481 aaagtaccgg gcgtgtacag tgattacaaa ttgtcaagtg acggcaaaga tgtagagagc
      541 attacatatg acattacgaa agaccaaaac caattaaata tcgcaaaagc agcactaaaa
      601 gcaaatcctg atttgtattt tgaaggattt tcaaattccg caccgtattt tatgactaaa
      661 acaggctgta caagcggcgg cggtactgta aacagtgacg gaacagttac gtcaaacgga
      721 aatttgaata acttaaatga cgatatgtat gatgattttg ccaagtttat tgcagacgca
      781 acaaagcttt tcaaagataa cggcatagag tttaagagct attcacctat gaatgagcct
      841 gatacggatt attggggtta cggctcacta aaacaagagg gctgtcactt tgatccgggt
      901 agaagtcagt caaagatgat tacggaaaca agaaaagcac ttgacaatgc aggatttgcg
      961 gacgtgcttg tggcaggtat ggacgaaaca attttgaaga aaacagcaaa caatcttgga
     1021 agtcttacag acgaggca
//
LOCUS       DAWACS010000372         1033 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            k99_1694_length_1033_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWACS010000372 DAWACS010000000
VERSION     DAWACS010000372.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1694_length_1033_cov_4.0000"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          339 a          212 c          232 g          250 t
ORIGIN      
        1 aaaatgtggt gccgaccttg ttataataga aacaatgtct gacactcttg aggcaaaagc
       61 cgccgtactt gcagtaaagg aaaacacttc actccctatt ttctgtacaa tgacgtttga
      121 cgaaagctat aaaacactta cgggcggtga cgtcaaggtt atgtcggcga ttatggaagg
      181 acttggggtt gattgtgtcg gtataaactg tggtcttggt cctgctcaaa tcggtgaaat
      241 gatgactgac cttgccgaaa tttcttcaat tcctataatg gcacagccaa acgcaggttt
      301 gccgcaaatc gaaaacggca aaacagttta caatgtatta ccggaacaat ttgcagacga
      361 atgtgaacat atggcaaagc ttggtgcgtc tgttctcggc ggttgttgcg gtacaacacc
      421 tgaccatata agaagtcttg ttgaaaaatg caaaaactac aaaccgatag tcgaagaaaa
      481 aaacatcact gtcgttgcgt catattcaaa aacggttgta ctcggaaaag gtcctgtaat
      541 tgtcggcgaa cgtatcaatc cgacaggtaa gaaaaagttt aaagaagcac ttagaaacgg
      601 cgacatagac tatattctaa acgaggcatt tgcacaacgt gacgcaggtg cacacatact
      661 tgacgtaaac gtcggactgc ctgaaattga cgaatgttcg acaatggaga aagcggttaa
      721 ggcaatcagt gccgctgtaa atcttccttt acagatagac agctccgacc ctgtaacaac
      781 cgaacgtgca ttgcgtattt acaacggtaa acctatggta aactccgtaa acggcaagga
      841 agaaagcata aaagcaatta tgccgatagt tcaaaaatac ggcggtgtac ttgtcggact
      901 ttgtcttgac gaggaaggta tccctcctac tgccgaacaa agatttgaaa ttgcaaaaaa
      961 aatccgtgac agagcgtcag aatacggcat aaagccgaaa aaccttgtaa tggacgcact
     1021 tactcttaca att
//
LOCUS       DAWACS010000373         1031 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            k99_5440_length_1031_cov_3.0000, whole genome shotgun sequence.
ACCESSION   DAWACS010000373 DAWACS010000000
VERSION     DAWACS010000373.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_5440_length_1031_cov_3.0000"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          349 a          202 c          247 g          233 t
ORIGIN      
        1 aatggagtca cagccgggcg gcgaatatca cccaaatcca aattcgtcaa caccgactct
       61 tgaccagttc ggacgtgact ttacagctat tgcaaaagag ggtaaatttg accccgtaat
      121 cggcagaagt aaggaaatcg aacgtgtaac tcagattttg tcaagacgta caaagaataa
      181 cccgtgcctt ataggtgaac cgggtgtagg taaaacagcc gtaattgagg gacttgctca
      241 gaaaattgcg tcaggcgacg ttcctgaacc gttaaagaca aaacgtcttg tatcggttga
      301 cctttcgagt atggttgccg gtgcaaaata cagaggcgaa tttgaagaac gtttgaagaa
      361 agttgtaaat gaagttcttg cggcaaagaa tgtaatattg ttcattgatg aatttcatac
      421 aatcgtcggt gcaggcaatg ccgaaggttc gatggacgcg tcgaatatcc taaagccgtt
      481 ccttgcaaga ggtgaattgc agcttatcgg tgcgacaacg cttaaagaat ataagaaata
      541 tatcgaaaaa gacgccgcac ttgaaagacg tttccaacct gtaacagtcg gtgagccgac
      601 agtcgaagaa acggttgaga tattgaaagg tatccgtgac aagtacgagg cacatcacag
      661 cgtaacaatc acggatgacg cattgaaagc cgccgcaaca ttgtcgtcac gttatatcac
      721 agacagattt cttcccgata aggcaatcga ccttattgat gaggcggcat ctaaaaaacg
      781 tcttggttca cagacagaac ccgatgattt gaaagaaaaa gaaaagaaac ttgaaaaatt
      841 gcagtcggaa aaacaagagg ctataactgc acaagacttt gaaaaagccg caaaaatccg
      901 tgatgaagaa aaggtattga aagaagaagt cgaaaaacta aaatcttcat ggaaaggtac
      961 aggctcatct tcgggacttg ttgtagacga ggacgatatt gccgacatct tggcagactg
     1021 gactcatatt c
//
LOCUS       DAWACS010000374         1022 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            k99_9198_length_1022_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWACS010000374 DAWACS010000000
VERSION     DAWACS010000374.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_9198_length_1022_cov_4.0000"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          264 a          235 c          213 g          310 t
ORIGIN      
        1 gtagggtaga caacttcttc tctccagtca ccgaaaaggt cggcttgaag tgacggattt
       61 gcttttgtgc cgttgattga aacacaatca taatttccgg cactgaatat attgctcata
      121 ttacggccgt tccaatcggt gatatttgca ccgttaagca agttgtcgta taaatctccg
      181 tcccagaaaa tacggaaatt cattgaaggt gtatttctgc ctgtaagtga agtggcagtt
      241 gtgaaatcag tgccgccgtt tgactgtctt gcagagtttt ttgcactcca aatttgatag
      301 tatccgccgg caccgacatt cgccataaca ccgcgacctg tgtcatcgga cgcaccttcg
      361 tggaacataa tattgcctgt ttcggcgtca ataacagaca taccgaaatc catagttatg
      421 tcattaccgc taagattgtt tgttccgctg tcctcgtgga ctgtgaaaaa ttcaaaaccg
      481 gtatgtgtag ggtcgtagtt tcctatatga agtgcgtcac cgtgctgtaa atatgtacac
      541 catttaggac gaaattcatt gtcgtcattg acttccatac ataatgcgcc tgtgataaat
      601 tcgtctttgc cgtcattatc cacatcggca acggtacagt tgtgattgcc gtttccgaca
      661 tattgataag caccgtcaaa atatccctct tgaccttttt gcgtatcaaa acgataagta
      721 ggggataaag ctgtaccgtc ccagcttata ccggcaatac ttgtacgctg tttgccgttt
      781 ctgccgaaat agtagcccct taaatatatt gcatacggct tttcgccgtc aagataagca
      841 atgtcggata caaaacgatt tgaacggtta ccgaaatcgt cgccgtaatc tacaccgttt
      901 tcacttccgc gagttgtcgg aagattaata gtgtccattg cgacacctgt ttcaccgttg
      961 aatactgtca aaaattcttt accttcggta attctgccgt aacctctgta atcggcagtt
     1021 ga
//
LOCUS       DAWACS010000375         1019 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            k99_13079_length_1019_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWACS010000375 DAWACS010000000
VERSION     DAWACS010000375.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_13079_length_1019_cov_4.0000"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          282 a          208 c          155 g          374 t
ORIGIN      
        1 tacagactgt ccgccgtact gattacttgc aaccgctgca ataatctgcg ttgtaactgt
       61 acaggcaacc tgaaaactct taggcgattc aatcatttta ccgttcataa cagtaccgtt
      121 gtcaagcata tccttgatat ttataagaca gcagttaaaa atcggctgta taaaataatc
      181 cgcgtcatga aaatgaagta tgccgttttg atgtgccatt gtgatatgct ccggcaacag
      241 catacgtctt gtaaggtcac gcgacacttc acctgctata tagtcacgct gtgtagacgc
      301 gtgaacagta ttcttatttg aattttcctc cgcaagctcc ttgttttcat ttctgattag
      361 ctttaggatt gattcatctg ttgtattcga ctttctcaaa agactgcgtt ggtatctgta
      421 tatcatatac tttttcgcaa gtacatactt gttgtgtgca acaagcgttt tttcgattat
      481 atcttgtatc gtttcaacgg gaattacttc cttgtctgtt ttttcaacgt ttcctatcgc
      541 ctcttctatc aagtcgtcac tgactttttg cgaattgtcc acctcaacat tcgctttttt
      601 aatggcggct gttatcttgt cagcattgta ttcaacaatt ctgccgtcac gcttctgtac
      661 tttcactttc ataacccctt ctctgtataa aacactatat acagtattat cgctcaaaaa
      721 aaatatgcag aaagcacgtc tttatgcgtg ttttatgcaa atctcaaata ctatgtaacc
      781 actatatttt gtgtccttga tatttttgta tatctatatc ttgtattata ttatagcaca
      841 gttggattat aaatgcaata cattttattt tacaaattga atacaatata aaaaagcaag
      901 cacaattttt atgcttgctt atcaatatat tgtatttata taaaataatc cggaataaaa
      961 gtttccctca tatcaatgta gcccttaaaa tctttttgcc gcttgccgtc tatcgtaaa
//
LOCUS       DAWACS010000376         1011 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            k99_17235_length_1011_cov_5.0000, whole genome shotgun sequence.
ACCESSION   DAWACS010000376 DAWACS010000000
VERSION     DAWACS010000376.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_17235_length_1011_cov_5.0000"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          322 a          155 c          241 g          293 t
ORIGIN      
        1 cttcctttac agaatagaac atcatattaa taccgaccat cacgatatgg gctttttgta
       61 cagtccgtct tgcgttgcgg catataagct tacaggcagt gaagtgggta aacgtgcggc
      121 acttttggcg gcggataatt tgatgaaacg ttttcacgaa aaaggtcagt tctttcaagc
      181 ttggggcaaa ataggtgata aggacaatta cagacttata atagattgcc ttatgaatat
      241 gccgttgctt ttctgggcga gcaaggtgac aggtgataaa aagtatgaag agaaagcaca
      301 ggcacatata aaaacagcga tggaaaatat cgtgcgtgac gataattcga catatcatac
      361 atatttcttt gatatggaaa caggtaaacc tacaaagggt gttacttgtc agggtaatcg
      421 tgacggctcg gcttgggcga gaggtcaggc ttggggaata tacggcagtg cggttgcgta
      481 ttctaaaaca ggaaataaag aatatttgga tatatttaaa cgtgtaacaa aatattttat
      541 ggaacatttg ccgaaggatt tagtgcctta ttgggacttt gattttgata ccggcagtga
      601 tgaaccgcgt gattcttcag caggtgttat agcggtatgc ggtatgcttg aaatggctaa
      661 atatcttgat aaagatgagg ctgaatatta tacgcagacg gctaaaagat tgcttaaagc
      721 aattattgat aattgtgccg ttaaagacag taaagagtct aacggacttt tacttcacgg
      781 tacatacgca aagaaaacac cgcataatac atgtaataac ggcggtgttg atgagtgtaa
      841 tacatgggga gattatttct atatggaggc acttacacgt ctgacaagcg actgggatcc
      901 atattggttt tagtatatag acaataaaaa aataccgtag tcgtaagact gcggtatttt
      961 tgatatgtcg taaaacttat ctctttgata acgttttgat atcttgaaaa g
//
LOCUS       DAWACS010000377         1007 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            k99_8999_length_1007_cov_3.0000, whole genome shotgun sequence.
ACCESSION   DAWACS010000377 DAWACS010000000
VERSION     DAWACS010000377.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_8999_length_1007_cov_3.0000"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          313 a          150 c          256 g          288 t
ORIGIN      
        1 atcttgttgc gtcggaggaa tatgagccgg gaacgggttg gtattacagt gaatggctta
       61 aaaatttgtc tgacaatacg tcaatgccga ctgtcgaaat aggtaagaag attgtggagg
      121 actatataaa cggtcctgat tcgagttttt ttgattcaaa tacgctttca atcgtagatt
      181 tgagagaaat tccttataca tattcaaagc tatgcgaaat aatgcagcag gaagaaggag
      241 ttttggacag cagatattcc gtaatatcac aggcaagata caatgcaaaa tcattcggtg
      301 acggtgagta tgaacagatt gatataaaat catatcttga agaatgcggc aagagtgata
      361 cggaactggg caaggtgctt agaagtgcgg ttaagtattc gggcagttcg tcttacaatt
      421 cgaacggact tgcaatgtat ttcccgtatg actaccccga ctattacgcg gaaataaaaa
      481 cagaaacaga taagttcggt tataacggct acaacagctt tttcgataga tttttgagca
      541 ttatgggtac aggacagatg aattattcga gtgagaatga ttacagtgat tattcttggt
      601 atgacaatac aaattacgat acctataatg tatcagatga gttggaagta aaggacaagg
      661 gtgattattt tgcattgtcg ctttcggacg aggaatggga taaaataaac ggaattaatg
      721 tatgggtgta tgttgatgac ggcgatggtt acgttgactt gggttcggac aatgtgtatg
      781 aatttgacga tgacggtgat ttaatggttg atttcgatta ttattgggtt gcattaaacg
      841 gtcaggttgt tccgttttac tttgaatacg aaacgccgga gaacgtaaag gacggataca
      901 catacggcta tgtccctgcg gtgcttaacg gcaacgagca gattgaaatg atgatttatt
      961 gggacgaaaa gcaccccgac ggttatttgg caggttacag accgtac
//
LOCUS       DAWACS010000378         1006 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate gSpreadC_ID_591
            k99_22048_length_1006_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWACS010000378 DAWACS010000000
VERSION     DAWACS010000378.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993094
            Sequence Read Archive: SRR3737002
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (human gut metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_22048_length_1006_cov_4.0000"
                     /isolate="gSpreadC_ID_591"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          327 a          165 c          203 g          311 t
ORIGIN      
        1 gttacagaga cggatattca agcggaagca gcaagtattc gtcaatgagc gattcagaac
       61 ttatgcgcaa agcaagaaat aaagactttt cgggaaacag ctcaaaacaa ataggtcagg
      121 caaaagctat gggtgacgaa cttaaaagac gaggcttgag atgaacgata aaaagtttgt
      181 agagtataaa tgctcatatt gcggcgcaac aatcactcgc gcgacaaatg ccggcagacc
      241 tatgccgggc gtttgcccta gaaaaggcaa aacacatgac ggtcgcacaa aaccgcatac
      301 atgggttatt aacagaaaat tttaaggaga gttacaatgt ataaaaagat atcagcgata
      361 gctcttataa tcataggtat aatgtcactg agaacatcac ttttcggagc ggcattctgc
      421 atattgttag gaatattttt attgactcat taatatcgct tattttggta actctatatg
      481 taaacttcct tttgactttg tcggttgaaa ggaagttttt tttataaaat tttttgatag
      541 gacgtaaatt gtccaagtgt gtgaatataa taaaagaaac ggagtacaaa aatctgtact
      601 ccgttcagtc tgtatcacta agggacaaag ctgttttaat caagcgatat atcttttcct
      661 tttcaacatc tgtgcggttt gaaagcataa cagtaatacg gtttaaaaat acatcatcat
      721 tgcttgcggg tttgtggttt gagaaaaatt ccgaaagcga aatgttgaat accgtacata
      781 acttttcaat cgtcagaatt gtaggatttt tttcgttccg ctctatttga cctaaatagg
      841 cgggagtgat attagcggct aaagcagttt gttcctgtga cagacgtgcg gctttgcgta
      901 aattgcgcag ccttagtcca atattatagc tcatagcatt caccaatttg tattttatag
      961 tatagaattt ttcttttcaa actgttatag tatatatttt tttaaa
//
