LOCUS       DAWAEA010000001        17182 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_532_length_17182_cov_2.544754, whole genome shotgun sequence.
ACCESSION   DAWAEA010000001 DAWAEA010000000
VERSION     DAWAEA010000001.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 17182)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17182)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17182
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_532_length_17182_cov_2.544754"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1644..1653
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2741..2993
                     /estimated_length=253
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8387..8396
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14075..14084
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14992..15001
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15320..15421
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3526 a         4898 c         5116 g         3247 t
ORIGIN      
        1 ccctactatt ttgcggcggt ggaagcgctg ccgctggcgg acgatctggt catcgaccga
       61 acggtctccc tgaagggcga cctgctgtcg ctgaaggtct ccatgcccta ctccgacccg
      121 gacatgggcg cggtcacgct gggctacagc ctgaccacga cggaagacag cgaacccagc
      181 agcgagatca ccgtggaaag tcaggagcgg ctggtcacgc tgcggtattt cacctatagc
      241 agcatgacgg acgtgagcgt cactcagggg acgctgatca gccagcccac cggcgaagcg
      301 gccttcaccg tggccgacgg cgagcaggaa aagccgctgg ccgtggcctt ccttttccgc
      361 agccagacca ccaacggcaa gggggacgac ggtcgggaca gctgccgcat ccagtgggag
      421 ctgagcctga cccccgatca ggagaccccg gacgcgcagg agtttgacga atacgacctg
      481 aacttggata tgacctttgc ctcccatagc ccccagaaat cggccaccga gctgaacgcg
      541 gtgctgactg tgggcggcgg ccagcggacg gggcgcactt tcaccctcaa cggaaccacc
      601 cgcacccctt gggtgcccga tgcggttccc tccgaaacgg tttccctgtc cgatctgccc
      661 gaaagtgact ggaacgctct ctggcaaacg ctgtggagcg acgcgctgct ttccaacctg
      721 aaagtcggcg agtgatccta tttacatgaa aaagccagca gagagtgaag tgccccctat
      781 ttgtcaggct gtgtgatata acgtctgaca aatgggggaa ttttatatgc ccagtatgcc
      841 tttcttttaa caccggattt ttctacttat gcagatatga gcttatggcg tcagatggaa
      901 agcgttgcga aaaacagatg gtgcggcgct tattggcaga gcaaaggact aaccgtcatt
      961 cctaccatca gctggagtac gccaagcagc tatgatttct gctttgatgg tgtagaggaa
     1021 aactctattg ttgctgtcgg aatgatcggc tgtaagcaga atcgtctgaa tttcatgcgt
     1081 ggatattatg ccatgctaga aaaaattcat cccgaaacca ttctttgttt tggaacaccg
     1141 tttccagaga tgcaagggaa tattgccacc gtagactacc gtgcctctcg aaaggtggtg
     1201 cgctaatatg ggtggacgtg gagcttttgc agttgggaat aatgttcctt acagctatga
     1261 aacagttgga acgatcgatg gagttaaagt attgcagggg acttccggga aacacagatt
     1321 accggaatca tctcactcaa gtattgccta tttgaagtta aacccagacg gaacatttaa
     1381 catgatgaga atatatgatc aatgtcataa tctcagactt gagatcgcat atcattcaga
     1441 acagtctatt gcaaagggaa agcccaaagt acttcattat catgtctatg gaaaagagtt
     1501 cagccaaaag gcaaaatctc cctttgaaag aacaacgaaa gtgcttcaca aaaattcaaa
     1561 gatttataag caatacaaaa aattttttaa gggggtgcaa taaaatgctt aatggaaata
     1621 tcagagaatt tgttgataaa ctgnnnnnnn nnnataacgg aaaaaaatac ttttctcaag
     1681 gatatatcct tgaaaacggt ctatatcgct ttgaattgca acaatgggag ccggaaggga
     1741 aaatgctctg gtatgtagaa ggtctggatc atcaatcaag tttagacaaa tttctgaaac
     1801 agcctttgtt tgatgggaaa agattttggg acgctgaaca agaaatggaa tgggtagatg
     1861 attagacaag ggagcaattt atgacaggga atactttaac tgagtttatg gatgatttgt
     1921 tggctatggg cggaccagaa aaggaatttg tgtttcgtgg gaaacgatac tttatggaag
     1981 cacagcctta tgaaaaagat attacacaaa cggaatttgc aatatttgaa tgttttggag
     2041 atcaaaatta catttttcgt tgccatggga aaaaataagg aatgtgttga gcaatttgaa
     2101 aaagcaaaaa tttttgatgg actaactatt tatgaagcag aaccagaaat tgaggtttga
     2161 ttcggataat gtagtaaaca aggaatgaaa atatatgatt ggtggtaatc ctaatgattt
     2221 tccggacagg atttattctt gtcaagatac catttatatt tacaagggaa taaaatactg
     2281 gtttcagggg tatatgcctg attcaaatac agtacacatg gaagtaatac aatatcaacc
     2341 agcaaacaat aaagaaatat ggagccatga tgccgccact attgagcagt gtaagcaggc
     2401 ttttatagct gcacctctct ttaacggaaa aacattctgg gatgccgaac aagatattga
     2461 gtgggtagac gattaagggt gtaatttata tttcatttat tttaaggagg tgcctaaaat
     2521 tgaaaaatgg tgatgttcgt gagtttgttg atcacattca ttatggagat gagctttggt
     2581 ttctctatga aggaaaaaaa tactttctgg aaggatggac gaataacggc agtcttgatc
     2641 tctgcttata cgaaatgaca gacaatggag aaaagcatat ctggaaaggg aacgcaacgc
     2701 attatccagt tgaagcattc ttagaagcta aaatctggaa nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtccggg
     3001 gtgcaggggt actacccctg ctgctgccgg aggcccttgg ggtagtggtt tttgagctgg
     3061 ccattggagg ccttggcaaa ccctaagggg agagattcgt agcagaccag ataaaacccg
     3121 gagaaggagt ccgggcaggg aagtacctcg ccccggagat acgcccgggc ttcagccggg
     3181 gaaacagaaa acgatgggaa tccggcagtc gggctcattg ccagcgccaa tgcgtgatcg
     3241 ggctgaaata ccttgccctt caaagccccc agctgtaccc cggcccgtaa gaccctgacc
     3301 ccctgtaagg gcggcagctc cggcgcagaa agcagcgcgt ctcctaaacg ggcattggcg
     3361 gccggcaacg ccgcgccgac ggctttggca aaggcatcgt aggcttgtaa aagcggcttg
     3421 tccggcttgg ccagagcatc ccccggagca agcgcggacg cgtgcggttc gtcgccgcgc
     3481 ttgcgcagga aagccacaaa atgcccttcc cctttgaccc ggtggggata gagatgcagc
     3541 gtcccgccgg gcgcgtccag ttccgcccct tccccggcag gaagggtaaa cgcggcggtt
     3601 tcgcagtcgg aaaacgcctg ctgcatccag tcaatcaccc cttcgttttc ctcggtattc
     3661 agggtgcagg tgctatagac cagccgcccg ccggggcgaa gcatggccca tgcggaggtc
     3721 agaatgcgct tctgccgggc cgcgcatcct gccggggact gctccgtcca ttcggcgcgg
     3781 gtctccgggt ggcggcgaaa catgccctcg ccgctgcagg gcgcatccac cagaatcgcg
     3841 tcgaaaaatc ctgcccaccg gggagcgagc tgctccggct cggcagaaac gaccagcccg
     3901 ttggtcacgc ccattcgttc cagattgcgg ctgagaattt tcgcacggct cggtaccggc
     3961 tcgttgctga ccagcagccc ccgaccctcc agcgcgtcgg cgatctgggt gctctttccc
     4021 cccggggcgg cgcagagatc cagcacccgc tcgccgggcc ggggagacag cactgccacc
     4081 gccgccatgg cgctgggttc ctgaatatag tacgccccgg cttcgtgcag gggctgcgcg
     4141 cccgccgtgc tttcctgcga cagatagcgc cccgtccggg gctgccacgg caccggctcc
     4201 cccaaccctt cgatcagggc cgcgggatcg gccagcggct tgcggggatt gagccgcagc
     4261 ccgcgcacat agggctgggc cagcgcggca aaatagtcgt ccgcttctgc tcccagctgg
     4321 gcgcggatcc gctcctgaaa aaccggcggc aggggatagg cttcgctcat ggggaacctc
     4381 cgttcaaaac atagactccg gggcataaaa gaaaggcctt atgccgaagg cccttcctgc
     4441 tgatggttgt tgggattctt gggatagacc ggttcgcgga atgccttgtg cacaggcacg
     4501 gtaggctgcc agtcatggat ggaacgggtg tcgtcctccg catccacgcc cacgaaatcg
     4561 cgggccttac gggccagcgc cgcaaaggga ttgcgaggtt tggcctgtgc gctctccacc
     4621 cgggagcgtt ccgcgtcggc aaaggagtaa aagtcgggcg caggctcctg cggggccgtg
     4681 tcggacggat tccagccgat gttgcgctga aagctggccg cgtccaaacc gggatggatg
     4741 ggcgcgttgt tttcataggg ctgagcagtt ggttcgttgg gcgtgtaacc gtttggcgca
     4801 gacacatttt gcgtctgcgg agcggcgtaa agctgctccg gcggcagggg acgggcaaaa
     4861 cccgcctgca tatcccggat gtagttcaac ggctctggtt tggcagcgcc gaaggtctgc
     4921 tccatgccct gcgccgcctg ctggatcgtg tccgggcgca ccagcgtgtc gccgtgctcc
     4981 catccaccca gatcctcgtc aaagaccggg tcaaaaccgg cgctttcatt gggcaccgcc
     5041 caagtcgcct gctggggctg cgccggggcg gacgggttct gctgccagtg ctcgtcctgt
     5101 acggcacggt gctcctcccg gggcagatag cccgaagcaa cgtaggaagg attgccgccc
     5161 cggtagacca tagtgggttc ctcctccggc aggggttcct cccactgggc aggcgaaacg
     5221 ggctggggat tttccaccgt agggacgtat tcatgagaga aatcctgccg gcgctgggag
     5281 cggcgggcct tgcgacggtt gcgccagacg gtgtcgggtc gccagcgcag aaaatatacc
     5341 agccggtcgg ccacaaagcc gcccaccgcc agacagacca ccagaaattt ccagttgctc
     5401 tgaagaaagg aaagtgcgcg gctgccgttt tcactgttca gtgcagccca gatctggccg
     5461 atcagcagcc ggatccaact cagcagcagc gacagaatgg aattggtaaa gccgttcatt
     5521 caccggcccc tttcccgaaa aattatttgg tcagaagcgt gacgctggcc ttggagggcg
     5581 tagccgtcca ggtcagggtg tccgggtcgt ccgcgtccag caccagcatg accggcaggg
     5641 cgtattcgcc taccgtcagg ccggaggcgt ccacataagc ggacatcttt gcggctgaca
     5701 gccgagagac cgcgttctgc gggccggtca catccacgtc aatggtgcgc tgggccagtc
     5761 cggcggacag tccctccggg atattgcgca cggacagctt tactccgctg aagctctggg
     5821 tggtgatgat gggctcgatc tccacctgta cggtcacggt atcggtgctc agataattca
     5881 ccgtggacgg cttgcgcaga ctcagctccg tggcgaagcc ctcgctgcgg tcgtcaaggt
     5941 cgatggccga agtggtgaac agggtgtcca gcgtatccag cgcggtgccg tcgcccgcgg
     6001 ccagcacggt aacgggcgtg atggtcacgc tcttgacttc atagcccgcg gcgggttcgc
     6061 cttccagccg aaccagatcg gaaacggtca gggtcttggt gggatagaga ttctgctcca
     6121 cggtgacggt gcgcagggtc acgcccgcgt tggtcacccg cagcagggag ccgtccagtt
     6181 cgttgccgtc cgcgtccagc acccgcatgg acagggcgga acgcacctgt ccctccttgg
     6241 cgctgagccg ggacaggtca taatccgcca cgatgctggc gatctgatcc acaatggtgc
     6301 gggggccgct gacggccgcc gtgctggggg ccagctgggg cgtggaggca taatagccgt
     6361 cgggcgcgct gccggtcacg ttcagcgtga tcggcacgcg gtagttggtc acatactcgt
     6421 ccaccaccac agtcaggctt tccggcgaaa cggacgtgac ggtgccgtag gtcgtggtag
     6481 aggaggtctg aatgcgcagg gtctgctcgc cagtctcggt gatacggctc acatccaccc
     6541 gggggttata gttggaggcc tgtgcgttgg aataggagcg ctggggcaca tccgcccgca
     6601 gccgcacgga aagattctcg tcctccaacc cggacaccac gatcagcccg ttgccgatca
     6661 gcgtgtccgt accggtgacg gtgacgggca cgtcgctgaa gacccgctcc cgggtcagtg
     6721 tgggatcctg cgtgatcagg cccgcccaaa ggcagaccgc cagaaaaagg gacagcagtt
     6781 tccagcccca gttatgcagg catacccgca aaaaacgggc gggcgcctga cgcagcgcct
     6841 ggcggacgct gccggtctcg tttcgcttgg gttcggtatt catgccgcgt ccccctttcg
     6901 ccgtttggtt ttctccagca ggctggacag aaggccggtc tctttttgct gataaatgga
     6961 ggtaagcacc tgttccagtc ccgcccggtc cagatggcgg gtcagatgac cctcccgggc
     7021 cacggagatg gtgccggtct cctcactgac gatgagggag atagcgtccg tggtttcggt
     7081 aataccgatg gcagctcggt ggcgggtgcc cagttcccgg ctgatgccct tgccttcgct
     7141 cagggtcagg atgcaggcag cggccatgat gcggttgccg cgaatgacca ccgcgccgtc
     7201 atgaaggggc gtgttgggct cgaaaatatt ttccagcagt gcggaggaaa tttcggaatt
     7261 gagggtcgtg ccggtctcca ctacgtcctt caggccgatg cgctgttcga tcacgatcag
     7321 cgcgcccacc cggcggcggc tcaggttcag catacagtgg gtgatctcgg agacgatctg
     7381 ggcgctttcg ctgcccgtgg cccgctcgcg gatggctccc cggccgatct gctccagcgc
     7441 cttgcgcagc tccggctgga acaggatcac cagcaccacc gcgccgttgg ccaccacgtt
     7501 ctgcaagacc cagctcagag cggtcaggcc cagaaggtcg ctgatccagc tggccgcgat
     7561 cagcatgacc agtcccttca aaacggcgct ggcccgggtc tccttggtca gcatcagcag
     7621 cttgtagatc agataggcga cgataaaaat atccagcaca tcggcaatgg tggggcgatt
     7681 gaaaacgttc cagaccaggt ttttgatctg atcccaagcg ttgttcacgg tcaatccctc
     7741 cctccgaaaa ttcttttgaa aagccctgtc gggctctatg cgcgtttctc ttgtgcgcct
     7801 tctttgatta tactacatat agaggtagga taaaaccccc cggcggcagt ttttcgcagg
     7861 aatttacggg ttttgcaggg tggaaatttt cggaaaaacg ctttcagaac atcaccttcc
     7921 tgcctatgga aagagtcttg acacccgcgt gccgcaatgc tatactccaa gaaagatgta
     7981 tcatgcatct gtactgattt ttcaacggtc tgtcaagata cgtttcggtt actaaaggag
     8041 gttttctcat gcagatccac gtttacgatc acgcccagca ggttgggcag gcggcggcga
     8101 cgctgctggc cgctcagctg atctccaagc ccaactccgt gctggggctg gccaccggct
     8161 ccacccccat tcccacctat caggccctca tcagcctgta caaggcgggt gtggtggatt
     8221 tttccagcgc gtcctccttt aatctggacg agtacgtggg cctgccggca gggcatccct
     8281 gcagctacca cagcttcatg cgggagcagc tgttcgacca tgtgaatatg cccgccgacc
     8341 acatccacat tcccgacggc aacgccgccg acccccagac ggaatgnnnn nnnnnncgat
     8401 caggctatca ctgcggcggg cggcatcgac atgcaggtgc tgggcatcgg ccgcaacggc
     8461 cacatcggct tcaacgagcc ggacgatcat ttcacctgcg gctgccacgt ggtgaacctg
     8521 accgaaagca ccattcaggc caaccgccgc ttcttcgaca gcgaagcctc cgtgccccgt
     8581 caggccgtca gcctgggcgt gggcagcatt ctggcctccc gctgcatcct gctgctggct
     8641 accggcgagg ataaggcgca ggccatccac gacacggtct gcggcgatat cacctcccgc
     8701 acccccgcca gcatcctcaa gacccatccc aacgtgatcc tgctgctgga tcgggccgcc
     8761 gccgggatgc tgtaaagcac atcaaaaaga gcgtctccgc cgtttcgggc agagacgctt
     8821 tttctttctt ttctcaaata tcgtacacga actgtaccct tggcagcagc cgctccagcg
     8881 tcgcattcag atacacgggt tcccgattct ggtcggcggg gacgatatag gcctctccat
     8941 cctcggcccg cagcatcagc agcagctttt ctccctcgtc gcaggtggca gccatgcaga
     9001 agctcagcgg cagctcgggc cgctgggcgg cgtaatccag cgcttcctga aatttctcct
     9061 ccggcggcgc ggagtcggta ttgcgctccg cttcaaacac ccggcggtac aggaagctgt
     9121 atgggctgcc agaaccggcc accggcagaa aaaaacggat ttccagaaac tggcacaggg
     9181 acagataatc cacaatactc tggtgaaact ccgccgtgtg ttcccccaac ggcgcctgat
     9241 cgtccttcca cgaaatgggc tgccagaaag cgtagcccgg acggcggcac ccgggaatga
     9301 ccagcgcttc gtccttcgga ctgcggagcc agcacagggg ccggcggccc gtttccttga
     9361 tcgcggtctg ctggaagcgg accagataat cacgcatgat aggcaataaa gccgtctgca
     9421 tgaacatttc cccccttcat ccgaccgatt tccaccaaaa agtctaccat tcgccccggc
     9481 ggctgtcaac cgttgcagcc gctgggcttt cgttttttcc ggcctttttc cgttccgaaa
     9541 aggaatgagt tgcgaaaaag cctgcaatca tgtataatta gcctgacctg ctgtgcgtaa
     9601 cgggcaggcc agcattgtgg gcgcaccgcg ccgcacaggg gaggcaagga cttatggaat
     9661 gtaagatcaa aaatgatatc ggtacgatct atatcaccga agatgtcatg ctgaaggtgg
     9721 tcggctacgc cgcgctggaa tgctacggca tcgtcgccat gtcctcaaaa cgcgccaagg
     9781 acgggctggt gcagtggttg ggacgggaaa acctgtccaa gggcgtgcag ctccgtctgg
     9841 tggacgacat gctggatgtg gatcttttta tcatcgtcga atacgggatt tccatcgccg
     9901 aggtctgcaa gaccatcgtg cagaccgtgc gctataagct ggaaagcatg accggcgtga
     9961 aagtccgccg cgtgaacatc tccgtggagg gcatccgggt gtaaggacgc tcgccgtagg
    10021 catacaaatt acaagcgtat cgggaggaaa tgaccttgat ccaagccaaa acgatcgacg
    10081 gggtgctgct gcgggatatg gtggtcgcgg gcgccgcgct tttggaaaaa caccgggaag
    10141 cggtggacgc gctgaacgtt ttccccgtgc cggacggaga taccggcacc aatatgagcc
    10201 tgaccatggc ctccgccacc cgggaggtca accagaagga atacctgaac gcgggcgagg
    10261 ccgccgcggc tgtatcgaag ggcgcgctga agggcgcccg gggcaacagc ggcgttatca
    10321 cctcccagct tctgcgcggc ttcagccggg cgctggccgg agtggagcgc attacccccg
    10381 cacagtttgc cgccgcgctg aaaagcggcg cggagaccgc ctataaggcc gtgatgaagc
    10441 ccaaggaggg caccatcctg accgtggccc gcgtcattgc cgaggaagcc gtcaagcagg
    10501 ccgagcgcac gcccgacgac tacgacgcgc tgttcaccaa catcctctcc accggcgagg
    10561 tcattctgaa aaagacccag cagatgctgc ccgcgctgac gcaggcgggc gtggtggacg
    10621 ccggcggacg gggcctcctg tacatctaca tgggttatgc cgccgtgctg cggggcgagg
    10681 agctgcccag cgacgacgag gccgagggcg tggaaggcga cagctacgag gacgagcaca
    10741 gcctgaccgg cgagctgaag tacatctact gcaccgagtt cggggttgaa aacctgttcc
    10801 cggacatcgt ggaggaggat atcgattccc tgcgccgccg cctgaaccgc atcggcgact
    10861 gcgtgcgcgt caccggcgac ctgagcgccg tgaaggtgca cgttcacacc aatgagcccg
    10921 ccaaggctct ggaatttggc ctgagtctgg gcgagctgat cgacctgaag atcgaaaaca
    10981 tggcccagaa gcgccgggaa gcccagccca aggaagaacc gcaggccgag ccccagcccg
    11041 ccgcccagca ggaacccccg aaggagtccg gcatggtggc cgtgtcgctg ggcgaaggct
    11101 tcaaccagat tttccgcgac ctgaccgtgg atcaggtggt ggagggcggc cagaccatga
    11161 accccagcat cgaggatatt tccgaagccg tgggccgggt caacgccaag gtggtctatg
    11221 tgctgcccaa caacggcaac gtgattctgg cggcccagca ggcggccgaa ctgtgcgagg
    11281 acaagaaggt cgtggtcatc cccaccaaga acgtggccat gggcatcgcc gccgccatcg
    11341 cctgccagcc cgagctttcc cccgagcagc atgccgcccg aatggaggaa gccgcccagc
    11401 gggtgcgcac cggcaccgtg acctacgccg tccgggatac ggatctggac gacctgcaca
    11461 tccatcaggg cgacatcatc ggcctgtata acggcaagat ccagaccgtg ggcaacgacg
    11521 tgcaggatgt gacgctgcgg ctgatggaag ccatcgtcac cgacgaggac gaactcatca
    11581 ccgtctacta cggcaaggac accaaagaag acgacgcgca ggccctgacc gacgagctgg
    11641 cctcccgcta cggcgactgc gacgtggaaa tgcagatggg cggtcagccg ctgtactact
    11701 atctggtttc tgtagaataa atcatggaac ttgcatctct gaccgggatc ggaaaaaatc
    11761 gatcggaagc cttacacgca gcggggattt tctcgttgcg tgatcttttg tacgccgttc
    11821 cccaaaaata ccgggactgc agcgccgtca cccccgccgc gcaggcggcg gcagggcaaa
    11881 ggcagtgctt ctccctcgtc cgggagggcg aagcgaagct gtcccgtttc aaggggaaaa
    11941 tgacgctggt cacctgctcc ttttccgacg aaagcggcaa gctgaccgcc tgctggttca
    12001 atcaaccatg gatgaaggag aacctgaacc gaggcacccg cttcctgctg tgcggcccgg
    12061 tggaaagccg gggcggacgg ctgcagctga tgaacccttc gctggaaaag gaaaaccgca
    12121 tcgtgcccct gtaccggccc atcgaaggat tgccccagcg ggtgcatgaa cagatcgccc
    12181 gagaggcgct ggaatcgctg gacgaggtct gcccggagac gctgcccctt tcgctggtga
    12241 agcgctatga gcttcttccg ctgagagaag ctctgcgccg tctccacgcg ccccggacga
    12301 tggacgaagt ccagcccgcc cagcgccgtc tggcctttga gcagatgctg atgtatcagg
    12361 cgggcatccg catgatgcgg cggctgcgcc ggggcggcct gcccatggag gctcccgccg
    12421 aacgacagga ggctttctgg gcctcgctgc ccttttcccc caccagcgcc cagcgccgca
    12481 cccttgccga gatcgcccgg gatatggaat tgaaaggctc agagccctac gccatggccc
    12541 ggatggtgca gggggacgtg ggcagcggca agaccgccgt ggccatgggg gccatgttgc
    12601 tggccgctca aagcggctac cagagcgccc tgatggcccc gacggaaatt ctggcccggc
    12661 agcactggga gaccatgaag cccttctttg agaagaatgg cttcggctgc ggtctgctgg
    12721 tcagcggaat gcctgccggg gaacggcggg aggcccttgc ggccattcag gagggccgct
    12781 ggcaggcggt gatcggcacc cacgccctca tcggcggcgg cgtgcatttt caacgtttgg
    12841 ggctgtgcat caccgacgag cagcaccgtt tcggcgtggc ccagcgcacc gcccttctgg
    12901 aaaaaggcgg cgacatctcc accgatctcg cccctcatct gctggtaatg tcggccacgc
    12961 ccattccccg ctcgctggcc ctgagcctgt tcggcgatct ggatgtaagc gtcatcgatg
    13021 agctgccccc gggccgtcag cctgtaaaga cccggctggt gccggaaaac aggcgcgacg
    13081 gcctgtacga gttcatccgg caggagttga aagccgggcg gcaggcctac atcgtctgcc
    13141 cgctggtgga ggaaagcgag accgccgacg gcctgatggc ggtaaaaagc cacgcccggg
    13201 agctaaagcg aaaggccttc gcagattttt ccgtggggct gacctacggc actcagcccg
    13261 ctgccgaaaa gcaggcggcg ctggcggatt tcgctgcggg aaagctgcaa atcctggtct
    13321 ccaccacggt catcgaggta ggcgtgaacg tgcccaacgc ctccgtcatg gtcatcgagg
    13381 acgcggaccg ctacggtctg gcccagctcc atcagctgcg gggccgggta ggacgtggca
    13441 cggcccagag ctggtgcttc ctgatggcgg agccaaacga acgcctgcag gcgctggtgc
    13501 gcaccaacga cggcttccag attgcccaga ccgatctgga actgcggggc ccgggcgaac
    13561 tgctgggcac ccgccagcac ggcgaagctc tgatgcccgg cgcgggcgac atgacccttc
    13621 tgtaccgcgc cagccagtgc gtggaggaga tcagcaccga ccccgccttt gcggaggagt
    13681 gggagacggt gcaaaagctg gccggagctt atgtaagcga atccgccaga cgggtctccg
    13741 tcagctgaac ggcggttgaa aacatctttg gtttcgggta caatacccgg gaggtgattc
    13801 catgcacatt gtgctttacg agccggaaat cccccagaat accggcaata ttgcccgcac
    13861 ctgcgccgcg accggctccg tgctgcatct catcaagccg ctgggctttt cgctggagga
    13921 caaatacctc aagcgggccg ggctggacta ctggcgcatg atgcgctatg aggtgcatga
    13981 aaattttgag gagctgctgg aaaaataccc cggcgcgcgg atgcactttc tttccaccaa
    14041 agcgccccga agctatacgc aggtgcattt cccgnnnnnn nnnntacggc ccggatgatt
    14101 ttctggtgtt cggtcgggag acccggggtc tgccggaaag tctgctcagc cgggtctacg
    14161 accgctgcct gcgcattccc atggtgccgg gagcccgcag cctgaatttg agcaattccg
    14221 tagctattgt gctctatgaa gccttgcgca cgctggattt tcccggaatg caggaagcgg
    14281 gcgcgctgac gggccgggag gacgaaagcg ccccgtggat ggattacgta tgaccgttcg
    14341 ttcaatatag gaacggaaaa ctcaagccct tcgaaaggag gaacggcgga tggacgatcg
    14401 aaccagacgg tatcagcccg ggcaggagga tgagcccatg gagctcaacg acgtattgga
    14461 gccctatcgt ggctccgaag acggcgctga ccgtttcgcc gatgacggcg gggactatga
    14521 cccttatgcc tacggagaaa ccaacgacga ccttgcctat caggacgaag cctacggcgg
    14581 cgatgacggc gatgaagccg ccgaggacgc gtgggaacac gaaggccatg tgcgcaccac
    14641 catgggcgtg ctgaacacct tcagcgtgct gctgggcgtt tttgtgattt ttgtgctggt
    14701 ggccatgctg ctggggctat tttcgtggct caaggcggac attctgcact cgctgaccct
    14761 gctggagggc ggcatccgct gacgaggccc gcgcatggct ttccatccgg cgcggcacac
    14821 taaacagcgg ctttcggccg aaggaggaaa gaccatggac gctatggcgc gttttcagcg
    14881 ggactgcacc gcgttttttc agacgctcta tcccggcggg caaagtccgc tggtgttcgg
    14941 cgcggggctg gcgaaaaagc ccgtgcttgc ctcgttgtaa cgcccgcaga cnnnnnnnnn
    15001 nctgatcggc gaggctcccg gcgagcagga agccttgcag ggaaaaccct tcgtaggcaa
    15061 ggctggcaag aatctggaag gctttttgga agtgctgggg ctgcgccgcg gcgaaattta
    15121 cgtcaccaat accgttaaat tgcggcccac gaaaatcagc gccgcaggcc gccgggtcaa
    15181 ccgtcccccc acccgggagg aaaaggagct gtttcgccca tggctgatga aggagatcgc
    15241 gctggttcgc ccggacgcgc tggtaacgct gggtaacgtg gccctgcaga gcttcatccg
    15301 agacacggta ggccacctgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15421 nctccccgcc ggaaaaagag gcgttcaccc tgcccctgtt tcccctgtat cacccggcct
    15481 ccatcatcta caacccccac ttgcaggagg actacgggcg cgaccttcag gcgctccggg
    15541 aatccctgcg tgtaagatca gacgccgggg atgacaaatg agaccttttc cggaggaatg
    15601 ggacgaaaaa tcgagacatt tggacgcttc catgctacaa tgatcccatc aaccgggcga
    15661 aacgctcgaa ggaggaattg cggatggaag catttttatt ggagaacctg ccgattctcc
    15721 tctgttttct tttcggcgtt gtcctgctga tcgtcgaagt ctttatgccg ggcttcggcc
    15781 tgccgggcat ttccggcatc attctggagg gcgcggctat tgtgctgacc tatctgtggc
    15841 acggcggact ggcggcgctg ggcgtgacgg tgttgatcct ggccattgtg gccgtagcca
    15901 tatcgctggc gctgcgctcc gccaaccggg ggcggctgtc caagtccaac atcatcctca
    15961 aggaagcgga aacggctgaa aacggctact ccgccaccag tgcggcggac atgcaggtct
    16021 tcgtcgggcg aaagggcgtg acctccacgc cgctgcggcc caccgggctg gcggagttcg
    16081 acggcgttcg gctcaacgtg gcctcgcagg gggacttcat tcccaaggat acgcccgtga
    16141 tcgtcgatca cgtggagggg gcttccgtag tcgtgcggcg gctgcctctc caataaggct
    16201 tcattcccat tttatcaccg caaggaggaa agaacatgga tcttctctgg gctcttgtca
    16261 actcttacgg ctacggcagc aacagcctgt ccgcgctgtg gatcgtgctg gtcattgtac
    16321 tggcgctgat cgtggtactg gttttcctgc gttttgtgcc cgtgggcctg tggatcacct
    16381 cgctggccgc cggggtacac gtgagcattg gttctctggc cggtatgcgc ctgcgccgca
    16441 tccagcccaa gctgctggtc aatcccctca tcaaggcccg caaggccggt ctggacgtga
    16501 ccctttccaa gctggagacc cactatctgg ccggcggcaa cgtggatcgc gtcatcaacg
    16561 ccctgatcgc cgcccagcgc tccaacatcg aaatgccctt tgaaaaggct tccgccattg
    16621 atctggcggg ccgcgacgtg ctgcaggctg tgcagatgag cgttaccccc aaggtcatcg
    16681 agacccccgt ggtcgccgcc atcgccaagg acggcatcga gctgcgggcc aaggcacgcg
    16741 tcaccgtgcg caccaatatc gagcgtctgg taggcggcgc cggggaagaa accatcatcg
    16801 cccgcgtggg cgagggtatc gtcaccaccg tcggttccag cgagacccac aagcaggtgc
    16861 tggagaatcc cgacctgatc agccagaccg tgctgaacaa aggtctggac gccggtaccg
    16921 cctatgaaat tctttccatc gacatcgcgg acgtggacgt gggccgcaac gtgggcgctc
    16981 aactgcagat ggatcaggcc gaggccgacc gccgcatcgc gcaggcccgc gccgaggaac
    17041 gccgcgccat ggctgtggcc cgggagcagg aaatgaaggc ctccgtgcag gagatgcgcg
    17101 ctcgcgtggt ggaagccgag gccgaagtgc ccaaggccat ggccgccgct ctgcgggaag
    17161 gcaagatcgg cgtgatggac ta
//
LOCUS       DAWAEA010000002        16497 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_562_length_16497_cov_2.411994, whole genome shotgun sequence.
ACCESSION   DAWAEA010000002 DAWAEA010000000
VERSION     DAWAEA010000002.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 16497)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16497)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16497
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_562_length_16497_cov_2.411994"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2088..2097
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7791..7800
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15048..15152
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3218 a         5044 c         4934 g         3176 t
ORIGIN      
        1 actcctgacc ccaacactgc cagtgttgtg cgctaccaac tgcgctaatg ccccgcactc
       61 aacgttggat atcttaccac gtttttctgc gtttgtaaag acctatttct catttttttg
      121 agaaaaataa ggacgtttct gcgaaaaacg atggaggatg caatcaattt gctttagaag
      181 agttcttgta aagcaatctg gctatccgac cttgtaaaat cggacagcca gattaagaat
      241 taggttcagc cttgtttatt catacatcac agggttgaaa ggcctcaaac agcaggtgct
      301 tgagttctat aattattccc actcaatact ggccgggggc ttggtggtga tatcgtacag
      361 gacgcggttg cagccgttga cctcgccgac gatgcgggtg gcgatcttgc cgagcaggtc
      421 gtagggcagc tgggccacgt cggcggtcat gaagtcgtcg gtggtgacgg cgcgcagggc
      481 cagggtatag tcataggtgc gctcgtcgcc catgacgccc acgctgcggg cgttggtcag
      541 gacggtgaag tactgggaaa tcttgctgtc ccagccggcc ttggcaatct cctgacgcag
      601 gatcaggtcg ctctcccgga cgatgcggac tttttcgggg gtcaactcgc cgataacgcg
      661 gatggccaga ccggggccgg ggaaaggctg acggtagacc agactctcgg gcagacccag
      721 agctacgccc agacggcgga cttcgtcctt gaagagcatc cgcagaggct ctaccagact
      781 ttcaaagccc agctccttgg gcaggccgcc cacgttgtgg tggctcttga tgaccgcgct
      841 gcccttgacc tgaccgcttt cgatcacgtc gggataaatg gtgccctggg caaaatggtc
      901 gcgcttgccc agctttttgg cttcttcctt gaagacctcc acaaagtcgc ggccgatgat
      961 cttgcgcttc tgttccggat cggtcacgcc cttcaggtcg gcgaagaagc gctcggacgc
     1021 gtccgcgtga accagctcca ccgggaaggt cttggtgaaa atatccacca cctgatcgct
     1081 ctcgccggcg cgcatgaagc cgtggtctac gtatacgcag gtcagacggt tgccaatggc
     1141 gcggtagaga agcgcggcgg ccacggcgct gtccacgccg ccggaaaggc ccagaagaac
     1201 ggtgccgtcg cccacctgct cacggatctg gcgtacggcg atgtcaatgt aggcttccat
     1261 gctccagtcg ccgcggcaac cgcagatatc catcaggaag ttttccagaa ggcgggtgcc
     1321 ctcgtcggtg tgggtcactt cggggtggaa ctgcacgccg tacatccggc gctgatcgtc
     1381 gcccatggcc gccaccgggc aggacacggt gtgagccagc acccggaagc cttccggggg
     1441 ctgcttcacc tgataggtgt ggctcatcca gcaatggctg gaggcgctca tgccgttgga
     1501 aaggggattg tgcacatcgt ctacctgcat gacggtgcgg ccgtattccc gctgcgcgcc
     1561 gcgttcaatg ggcgcgccgt gcagatgggc catcagctgc attccgtagc aaatacccag
     1621 gaccggcaca cccatccgaa aaatttcttc atctacccgg ggagaatccg gatccaatac
     1681 gctggccggg ccaccggaga agatcacgcc ctgcggttcc aacgcccgta cttcctccgc
     1741 cgtcgccgtg tagggcagca ccagggaata tacgttcagt tctcttactc gcctggcgat
     1801 cagctgggta tactgtccgc caaagtccaa aatgactacg cactgcggtt tttccgccat
     1861 gtctgcttta ccccctcgtt attctgtcgg gacttaaaaa ttatagtgca aaaacggaag
     1921 aagggcaagg ggatgttttt ccgtttcggc cggttgattt ttttctgttt ggcaggattt
     1981 cgcggctgtt atttcccggt gaactatgct acaatgacat cgacctgccg gatgcgtccc
     2041 cggcggggat tttgctaaag aaaggatgct gcctatgccc aatttttnnn nnnnnnngtg
     2101 tttctggccg cggcgcagca gtaccagacc ccctttcacc tctatgacga ggcggccatc
     2161 cgccgcaccg cccgccgcct gaaagccgct ttttcaggcg tgccggagtt tcaggagtat
     2221 ttcgccgtca aggcgctgcc cacccccgcg attctgcgca ttttgaagga tgagggctgc
     2281 ggtgtggact gcgccagcat gaccgagctg atgctggtgc gcgcctgcgg ctttcagggg
     2341 cgggacatta tgttctccgc caatgatgtg ccgccggagg agttccggct ggcccgggaa
     2401 ttgaacgcct atatcaatct ggatgacgcg acccacgtgg ccaccctgcg ggataacggc
     2461 ggcattcccg aagaagtctg cctgcgctat aatccgggcg acgcgttcac cgtggcgggc
     2521 gagatcatgg gtgagcccaa ggaagccaag tacggcatga cccgggcgca gctttctcag
     2581 gctctgcggg agctgaaagc gggcggtgcc aagcgcttcg gcctgcattc cttcctgtgc
     2641 agcaatacca ccgacccggc ctattacccg gcgctggccc ggctgctgtt tgagacgggc
     2701 gtggagctga tggaggagac cggcctgccc ctgagcttca tcaacttgtc cggcggcatc
     2761 ggcattccct atctgcccga cgcgccggag gcggatatcg aggccattgg cgcgggcgtg
     2821 gcccgggaat atgacgaggc ctttacccgc cggggcattc ggggcatagc catcaagacc
     2881 gaactgggcc gctatatgac cggccccaac ggctggctgg tgacccacat cattcatgaa
     2941 aagaacattt accggcacta tctggggctg gacgcttcgg cctgcgacct gatgcgcccc
     3001 gccatgtatg gcgcgtatca ccatatcacc attcccgccc gggaagacct gccccacgac
     3061 cagctggtgg acgtgaccgg cagtctttgc gaaaataacg acaagtttgc cgtcaaccgg
     3121 ctgctgccca aggcggaggt cggcgacgtg ctggtgatcc acgataccgg cgctcacggc
     3181 ctgagcatgg gctacaacta caacggcaag ctccgctgca aggaagtgct gcgccgggag
     3241 gacggctcgt tccagctgat ccgccgggcc gagaccccca gggactattt tgccacgctg
     3301 gacgtggacg aggtctaccc caagctggga ctggactgac cgctgaatga aggaggattt
     3361 gcgaatgcga aagaaagcgt tcctgtgcct tgtgttttcc gccctgctga tctggggcgc
     3421 gctgctcccc gctctggcgg acgaggaagt gaccctgacc attctggata cggaggaggc
     3481 ccagattccc gaagcggccg aaaccgcgga gcctgccgag acccagccgc ccgtgggcaa
     3541 cgcgtccgcc gggggcgatc ccgccgcccg ggaggccttt atcgacggca ttatcgccat
     3601 ggcccagaag gaatacgaac aggcgggcgg caaggcgcac gaggcccagt acgccagcag
     3661 caactatgtg tgcaaaaact acaccgtcta tctgttccgg gccaacgcag gttcgttccg
     3721 tatggcggaa tttcccaacg tgcccctgaa aatcccggat aataagcccc gggaggaatg
     3781 caccgactac gtgtacggcg tggaatggaa ggatgtaccc gccagcgagg gcaatccctt
     3841 ctacgcggcg gcctctttcc gctacgatac gtccctgagc gcggaggaaa accggcagaa
     3901 ggcgcgggcg ttccttcagc aggtgcagaa gggcgactat ttccagatgg ccgccaacta
     3961 ttactacggc gtgggcgcgc acagcatgat cttcattgcg gactacgacc cggacacgga
     4021 caccgtccgc tggtgcgaca gcaacatgaa gggcgaaaag cgcaacggca tccgctacgg
     4081 ctatttgcag ttcgatgcgg tgaagaagat cgactggttc gtggacgctt tctgccgcaa
     4141 aaagtacggc gcgaccatct accgcctgcg ggacgatatt atccgggtgg cgcagtaggt
     4201 ctgtttggaa cggaagaaga aaggaaggga aacctgatga aatatgtgct tgtgatcggc
     4261 gacggcatgg ccgataccta tctgccccag ctggcgggca aaacgccgct ggaggcgctg
     4321 gcgctgaacg cttttgaccg ggcggcgggc tgctggtgcg gccgggcgct gacggtgccc
     4381 cacggcctgc ccgcaggcag cgacaccgcc attctcagca tttttggcta cgatccccgg
     4441 aagtattata cgggccgcag cgtgctggaa gcggcgggca tgggcgtgaa ggtgcctcag
     4501 ggctgcgtca gcttccgggt gaacctgtgc gccgtcacgc cggacgaaag cggcgcgctg
     4561 gtcatccaca gccacaacgg cggcaatatc cacgggcagg aggccgtcac cctgatgaac
     4621 gacctgctgg ccgatcccac cttcgcggcg atgcagaagg acgcggggct ggagatcacc
     4681 gttaccgata ccttccgcca tgtgggcgtg atgaaaacgg acgcgcacga cctgtccgcc
     4741 gtgcggctga ccgagcctca caacgtgctg gaacagccca tcgagcccca tctgccccat
     4801 ctgctgcggg agacggagga cgctgccgcc cggaagatgg ccgaggatat caaggccctg
     4861 atgctgcgca gctatgaagt gctgaaggat catcccatca atcagaagcg ggtggccgag
     4921 ggcaagctgc ccgccaacat gatctggccc tggggcgcgg ccacggccat ggaactgccc
     4981 tccttcaagg aaaaatacgg ccactggggc acggtggtca gcgccgttcc gctggtatgg
     5041 ggcatcgccg gtctagccgg actgaaaacg ccccatgtgg aaggggccaa cggcgatctg
     5101 gacaccaact atgagggaaa agtagcctgc gcgctgcagg cgctggccga cggggacgat
     5161 tttgctgcgg tgcacatcga agcaccggac gaaatggctc acgcgggcga tctgggccgc
     5221 aagctggaag ccatccagaa tgtggaatat cgggtcgttg gccctattct ggacgagctg
     5281 accatgcggg gcgaggactt ccgcctgctg gtgttgagcg accatcccac cctgctgacc
     5341 acccgcaccc acgacggcgc gcccgtgccc tatgctatct acgacagccg cacggtgaaa
     5401 aaggcgctgg aaaaggacgg ccgcgcgccc ctgcgcaagt tctgcgagcg ggagatggag
     5461 tccgaacccg tgctgatgga cggcaccgac ctgatgcggc tcttgtttga acaaatctaa
     5521 gggaaatccc aaaagcccgc ctttccatgc ggaagggcgg gcttttagcg tatggaataa
     5581 gctgcggaag ctcatttctt acgggttttt cggctcagga ctgcctgtac gccgagcaga
     5641 ctcagcgcgg ccagtgccag cagggcccac aacacaggac gactttcgtc tccagtggca
     5701 ggaggagtgc ccggaacagg ggttggagac ggagttacgc ctggggtcac ggtcggactt
     5761 ggacttggcg ttgccgtagg agaagcgtcg tacacattgc ggaaaaccag tttcggttct
     5821 ccctgcgccg tggccgtcag ctggtcgtct acgtattcga ccaatacctc caccgtataa
     5881 gacgcgccgt catacgtaac attcgacaga tcgccctgta cctctcggat ggtatagcgg
     5941 tgcagcccca cgtcctcgtg acggtactcg atatagttga aggcgatgct gccgtccgcg
     6001 tgattgacgc cccgggagac ctccgtgcct tgttcgtccg tgacgataaa atggaactcg
     6061 ttggccagca gggcgcggcc ctccagcagc ttgattccct tcagctggat ggacgtttgc
     6121 acgggcattt ccgccagacg ggtacacagc acattctgat actgttcatt gcggttgcgg
     6181 tacagatcca gctgataacc gagcggcagg gtatccaagg gcgtaatcag ctcctgataa
     6241 gctgcccacg ccccgggata ggtatcaaac ggctgatact gctggtaatg ctgggaaata
     6301 tccagcgagt ccgtataata ccagaccgcc agctgcgtaa tgcagcggaa ggtggcacgg
     6361 gttagaccgt atttttccat caggcctgat ccgttttgtg ggaaaccctg atagcagacg
     6421 gacaatatgg cctttttgaa cgcttcgggt tccatgaccc ggacgttttc gtcggtcatt
     6481 tggtagaact cttccgccgt ggcgctttcg atcttgtaca cggtctgtcc gccctcctgc
     6541 caggtaggcg gtactttctt cgaggcgttg taacagtacg ctacgcgatt ctctccgttg
     6601 ttcaggacgt acagcggatc tgaatacttg tattcctccc ggtcgcccgt gaaaacaatg
     6661 aatgattcat ccgcagcggc aaggcctgtt ggaatgacca tcgtcaaaac aatgaacaaa
     6721 gcaatcagaa actgaacggc gcttttcaca cccatcctcc ttccaagcat aaaatgtgac
     6781 attattttaa taaatagaaa acgaaattgc aatatgtaac agaatttttt ggagaaaaat
     6841 tttcaagcta tatgggaaaa acggtggaaa aggaacgaaa ctgcggctgt tcagcccgtt
     6901 gctcatgaaa aaaacaaggc tcctccgtca ggccggagga gccttgtttc ataaccccat
     6961 atttttgcgc atatggacca gcgcgttctt ttccagccgg ctgacctgcg cctgcgaaat
     7021 gccgatctcg ctggcgacct ccatctgggt gcggccctga taaaaccgca gcttcagaat
     7081 gtgccgttca cgctcgttca ggcggctcat ggcgtcctgc acgccgatct cgtccagcca
     7141 gccgttgtcc ggcttgcgct cgttggtgat ctgatccatg atgtacagcg agtctccccc
     7201 gtcgttgaac acgggctcga acaggctgac cgggtcctga atggcctcca gcgcaaagac
     7261 cacgtcctcc cggggcaggt gcagggcctc ggccagctcg ttcacgttgg gctcccggcc
     7321 cagcttttcg gacagctgat cccgggcttt cagggccttg taggcaatat cccgcaagct
     7381 gcggctgacc cggatggcgt tgttgtcccg cagataccgg cgaatttcgc cgatgatcat
     7441 gggcacggca taggtggaaa agcgcacgtt cagcgtggta tcgaaattgt ccagcgcctt
     7501 gatgagcccg atgcagccca cctgaaacag atcgtccacg ttttcgcccc ggttgccgaa
     7561 acgctgaata atgctcagca ccagacgcag atttccccgg ataaattcct cccgggcgtt
     7621 gagatcgccg ctgtgcacca gcggaaacag ctcccgcata cgctcgttgg tcagaacggg
     7681 cagggtagac gtatctacgc cgcagatttc caccttgttg atcgccatgt tggtttcctc
     7741 ccttgccggc tttcaacagg aagtatgggc aaaagggagg gttttcattc nnnnnnnnnn
     7801 cattccgact cgggcggaaa ggacaatcaa agggaaaacc cgacagaaaa caggtttttt
     7861 ccattcaaaa atcgaaaaga tacctgtcac taaatcgtct catcggaaag gggagaagca
     7921 gaatgaccca ggaaacggaa atcctgcgcc agtggatcgt ggaggcacga aacatcgtct
     7981 ttttcggcgg cgcgggggtc tccaccgcca gcggcatccc ggattttcgc agcgcccatc
     8041 agggcattta caataccccc ggcggccgca gctatgagga gatgctttct catgaatatt
     8101 ttgtcactca ccccgacgaa ttttgggatt tctaccgccg ggtgatgctc tacccggacg
     8161 cgaagcccaa cgccgcccat ctggcgctgg cggagctgga acgccggggc aagtgcaagg
     8221 cggtcgttac ccagaatatc gacggactgc atcaggcggc gggcagtgaa aaggtgctgg
     8281 agctgcacgg ctccgtccac cgcaaccgct gcatgaagtg cgggcgtttt tatccgctgg
     8341 agccgctttt gcagggcgag ggcgcgcccc actgctcctg cggcggcgtg atcaagcccg
     8401 aggtggtgct ctacggcgag ccgctggacg acatggtgat ggaagaagcc ctgagcgcca
     8461 ttgcccgctg cgacctcctc atcgtgggcg gcacgtcgct ggtggtctac cccgccgccg
     8521 gtctgatccg ctaccgcagc ccccgctgcc gtctggctct gttgaatcgg gacgcgaccg
     8581 actacgaccg cgccgccggt ctggtggtgc gggacgacct gacgcaggtg ctttgggacg
     8641 cggtagaaaa agcataaaag aaagcgggaa tgtctccata tttgagacat tcccgctttt
     8701 gatctgtatc ttcctttttc tgtgtagaat gccgccccgg catttcgcca attttgctgc
     8761 ggtttgtttt ccctgcggaa acggactgcg aagcggctga ggcgctgtcc tttcccggcg
     8821 gagaactgag ccagtgttga ggctttagag cggaagggtt tcttttccac cagcagctgc
     8881 actccgggcg cgcggcagta gccgccatgt cggaaaatgt ctgtccgctg ctttttattt
     8941 cagcagctcc agcagctccg ggaagggcga cagcgagcgg cgcaggatgc cgtgcatctg
     9001 ggcgatggcc acgccgtaat tggtgatggg caccccggca gccacggccc gctccacccg
     9061 ggactgcatc tcccggtcgt tgagcataca gccgccgcag tggacggcca gcgcgatgcc
     9121 gtcaaagtcc tccggaaatt cgccgccgga agtgaagcgg aattgcaggt cttttttgcc
     9181 ggtgtagccg ttgatccacg caggcagctt caccgtgccg atatcctcgc actggcgatg
     9241 gtgggtgcag ccctcggaga tcagcaccgt atcgccatcc cgcaggtgat ccagcgccgc
     9301 cgcgccccgc accatctgtt ccagctggcc cttgtagcgg gcgaaaagga tggagaagga
     9361 ggtcagggga atgtcctcgg gcacgtcccg gctgaccggc ccgaaggcct gcgagtcggt
     9421 gatgaccagc cggggcttcc ggcccagcgc cgccagcgcg gtcttaagct gcggaacctg
     9481 taccaccacg ttcagggcgt gatggtccag aatgtcccgg atggtctgct gctggggcag
     9541 gatcagccga cccttgggcg cggcggagtc taccggcgtg accagaacga cgatatcctc
     9601 cggctgcagc agatcgccca cgatgacccg ctcatttttc ggctggcgca ccacggcggc
     9661 aatggcttcc ttcaattcgt gaatgtggtc gccccgctgg gcgctgacgt agagctggtg
     9721 ctccccggcg gagggaagct cccgcagcag gtcgcatttg ttcagggcga tcaggtaggg
     9781 taccttccgc tcctgaaaca gcttcagcag ggcttcgtcc tggggctgca ggccgcgcac
     9841 cgcgtccacc accagtacgg cgatgtcggt cctggccagc acctgccggg cccgatgtac
     9901 ccgcatttcg cccagttcgc cctcgtcgtc catgccggga gtgtcgatga tgaccaccgg
     9961 ccccagcggc agcagctcca tggccttctg caccgggtcg gtggtggtgc cctttacgtc
    10021 ggagaccagc gaaaggttct gccccgtcac ggcgttcacc acgctggatt tgcccgcgtt
    10081 ccgcagaccg aaaaagccga tatggggccg gtcggcggag acggtcgtgt ttaggttatt
    10141 catgaaaaaa tcctcctgaa aaacagaata ggcgttccgc acaaaaatcg gaacgccttt
    10201 cttttccccg cgccggacgg ttttccctgc cgggagcccg gatagacact ggcttttttc
    10261 tgtttttaca gtcccgccgt cagcgcgccg cgcatttcct cccacacctc cgcgccgtcg
    10321 aagtcctcgg cttccagctc aaattccttc tcccagatca cgcggccctc gtaaacgccg
    10381 tcgggatagt ccgtcaccgt ggcgcggaga atccggttgt cgtagacgat ggcgaagctg
    10441 tagctgccgt cgggggaatc ctgccagcgg gagctggttt cagccctcag cacccgcacg
    10501 ggcgctagct ccgttccctg ccagcggtac acgccgtact cgcattccag ccccgccatg
    10561 ccgttgttgg cctgagtcca tacataaccc ctgtccgcat ccagcccgta gttggccagc
    10621 ccggcctcct gcccggggga cgtggcgtag acgtactgcc gcccgtccca gacgaagaaa
    10681 tcataaaatg cgttgctggc ccccagccgg gtgagcgcca ctacatcctc cacgccgtcg
    10741 aagttcacat cctccctgtg aagcacgggc tgctgccgct cgtctgcgac aaggtagatt
    10801 tcctgcccgt cgggggccgt atagcggtag atggcgccgc cgccggtaag ggccagcttt
    10861 tcaacgccgc tcccggcggc ctctgccccg gccccgcagg cggccagact ccccagcagg
    10921 cacaggacgg ccagccggga aaggaaacgg cggaatggga aacgcttcat gaaaaaacct
    10981 ccccgctctc attttctggt acggacatag aacgaacgaa caagaaaaag ccctgccgaa
    11041 gcagggcaaa aatttaattt ttttccagtt cttccgctac cagttcggcg cagaaggcca
    11101 cgtactgggc acaatggcgc tttttgtaat actctggcgt gcgctccgag ggcacgttgg
    11161 aaacggaaac gccgtttttc tccaaaagct cccggcagac ggaagtgccg tatttttcca
    11221 caaaacgctc atgcagctgc tgcacctgcg cgtacaggcg cttcttgccg tccagatccg
    11281 ctccgtcggt gtagccccgc agggcgctca acaccatgac catcgccgtg aacgcgccgc
    11341 aggtgaaccg cagcccgccg aggccgccgc ccagtccgct ggacaaaagc agggcccttt
    11401 cccggctcat gcccatgtcc tcggcaaagg cggcgtacac cgactgggcg cagttgcttc
    11461 ccgacaggaa cagctctcga gccagctccg ctttttcgtt gggattcatg gcttacttcc
    11521 gctcccgggg ctggagctgg tcgatggcgt tggcgatttc atccagccag tcgccgttgg
    11581 tcagctcgat catgcccttg tccgcactgg cggccagctt gcggtcgatg ggcagggcgg
    11641 cggtgtgggg aatgccgtac tgcttggcga tctcgttcac atggctctcg ccgaagacct
    11701 cgatcttctt gccgcagtcc gggcactgga cgtagctcat gttctccacc agaccgatga
    11761 cggggatgtt cagcatatcc accatcttga tggatttttc cacgatcatg cccaccagct
    11821 cctgaggcgt ggaaaccagc accgcagcgt ccaccgggat ggactggcac acggtcagca
    11881 tcacgtcgcc ggttcccggc ggcatatcga tgaacagcac gtccttatcg ccccagatca
    11941 cgtccgtcca gaactgggtc acagcgccgg cgatgatcgg gccgcgccag gctacggggt
    12001 cgctgtcgtt gggcaaaagc aggttcaggc tcatcacgtc gatgccggtg gtggtgtgca
    12061 cggggaaaat gcccgtgtcg ttgccctcgg ccttctgctt caggccgaat tcccggggaa
    12121 tggacgggcc ggtgatgtcc gcgtccagaa tgcccacctt caggcccatg cgctgcatgg
    12181 tgacggccag cagagacgtg accatggatt tgcccacgcc acccttgccg ctgcacacgc
    12241 cgatgacctt tttgatgtgg ctctgctcat ggggcttctg cagcaggctt tccggttcgg
    12301 tgcgctggcc gcagttctgc ccgcaggtgg agcagtcgtg tgtacattcg ctcatgattt
    12361 gtttccctcc gtaagtgatt gtcggaaaaa cccgctttca gcaggctttt tcgcaaaaga
    12421 ccgttttact cgtccgccgc ttccgcctgc ccggccagcg aggccgccag atgcgccgcc
    12481 tcccggatca ggctgtccag ccgctgctga cccaccccgg ccacatacag gccgcagcgc
    12541 tccagcggca ccagcacctt ctgggcttcg ctcaggctga tggccatggc gatggcggga
    12601 gacacctcgc ccagcatgga attggcgttg agcagaccgg taacgcccac gatgacgtcg
    12661 gcgcttttgc actgatagcg gatggcgttt tcgccggtag cgccctgatc cgcgcccgct
    12721 ttgagcatgg cggcggtggc ctgggtgttg gtgcccagcg cgatcagggg ttgagacggc
    12781 atttcccgct tcagggccgc gatcaggctt ttgcccacgc cgccgccctg tccgtcgatg
    12841 accagtatct tcatgcgtcg ccctcccagg gcatactttt gagccggatt ctggcccggc
    12901 tctgtatatc atacgcctga aaataagaat tttcaagggg gatccacact tcccggaacc
    12961 gctccagcag ggcgggactg cgtttttcga tacgccgcag ctgctcggcg ggggaaacgc
    13021 acagaaagac ggtcagcgcg tccgcagccc ggtagctttc ctcaaaacgg gggtgaaggg
    13081 cgtagctgcc ctccacgatc cgcacagggc tttggggaag gaagacggga cgggtcacgc
    13141 cgtggtggca gtcgaagctg ccgtaggaga aggccctgcc cgctatcaag ccgggcagca
    13201 cttccgaaag gaaacgttcg tagtggatgt tcccgccggg ctgattcagc cgttccgggg
    13261 tgcgcagctc cggcggcagg aaaaattcgt ccatgtggac aaccggcgcg ccgtacaggc
    13321 ggctcagttt ttcggccagc gtggtcttgc cggagccgca ggggccgtcc agcaccgcca
    13381 gcacgggcct gccctgtgcg cttcggtctt cgatggcgga aagcagcggc agcagaacgg
    13441 caaagtcgct ggaaaccacc cgataggcgg gccggtatgc cgcccggtag cggggggaat
    13501 ggctcgccgg gggataaccg gccgcgcggt attcggccag atagccgtcc agcgcagccg
    13561 ccgaaaaggg agccagcccc tcctgagcgg ctttccgcag cagggccagc tcccgttcaa
    13621 agcccggcag acgctcgtcg tcctccgtca tcggcggcac gtcctccgcc gtcagggtca
    13681 tcatccgggc cagccgctcc ggcggcaggg cccggacggc gggcgcagcc agattcatcc
    13741 ggcacagccc gtttccgatg aaggtaaaag gaggaaggtc ggcgcttgcc ggcgtttcgt
    13801 ccgcctctgc ccggatccgt tccaccagct gaccgtccgg cggcagcagg tggccgcagc
    13861 cgaaccggct ttggtacagc agcttcacgc cgtcgcaggg ttccatttcc ggcgtttctg
    13921 ccagccgttt atgaagcagc gcttcggtca tgctcatgga acgcgcctcc cttccgttga
    13981 tttcttccta acttattgct gttggaacag cttggccttt tccagcgcca gcacgatggc
    14041 ggggatcagc acgatctgca gcacgatgcc cggccatgcg ttgacgaagc cgcccatcca
    14101 gaaggccgcc agcgtgaagg cctgctccgt cagcagaccg tagatcggaa tgctgaccag
    14161 tccccatacc agacggccgc ccaccagcgc ggccagcagg gaaaggtaaa cgcccttcac
    14221 gccgccgtgc cagtggctgt agcatacgcc cgccagcgcg ccgtaggccg ccagttcaaa
    14281 ggccatggcc agcgccgtgg gcaccatggg cggcattccg aacagcacgc tgcgcagaag
    14341 gggcgtcaca aagcccacca gcaggcccca tttccagccg cacacaaagc cgcacagcag
    14401 caccggcagg tgcatgggca gcagcatccg gccaaagttg gggatgccgc ccgtcaggaa
    14461 cggcagcacc agccccgccg ccagcagcag cgcggagatc acgagacgtt tggttttcgt
    14521 agacatcaaa aaatcatcct tccgtcatga agacatgcaa aattcccttt tctcccttct
    14581 tcggaaggga aaaaggacgc cttcgtgaca tccggataaa aaggttggtt ccgattataa
    14641 cagagttttc ggcaaatgtc cagaacgccg cccgttacgc gccgaagcag gtgaacatca
    14701 cggtcagata gacgatgtac agtcccccgg ccagaaagcc catccagcgc tggaacttgc
    14761 ccttccacag ggcgggaacc agcacggcgg cgcagatgat gagacaggcg ggaatatcca
    14821 gcagcattcc ctgactctgg acgggcagag gcttgccgag gatcagagag cacaggggca
    14881 ggatcagggt cagatccatg atgttcgcgc cgatgatatt gcccacggac agggaggact
    14941 gcttcttgcg gatggcggtc agggtggtca ccagctccgg cagggaggtg ccgatggcga
    15001 tcatggtggc ggcgatgatg gcgtcgggca cacccagcag ggacgcgnnn nnnnnnnnnn
    15061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccgccacg gttttgccgc tgacagcggg
    15181 gcgctcctcg tccgccttcc gctccgtccc ctgctcccgg cgggcggaga tcaggctttc
    15241 cgccagaaag cccgcgaaaa tcaccagaac cagcagaccc tcggcgacgg aaagccggcc
    15301 gtcccgggtg aagccgaaca gcgacagaat agccgccagc agcaggcagg ccttcacgcc
    15361 gaactgcctg cgctccatca gacacgtcat gcacaccagc gacaggcaca taatcagccc
    15421 cgtattggcc gttaccgagc ccaccgcgtt gaccacggca atatccgcgt tgccctccag
    15481 cgccgcaaag acggacacca gcatttccgg cagggtggtg gcgaagctga cgatggtcgc
    15541 gccgatgatg aatttgggaa tgccggacgc ttcggcgatc catccggccg cgtccacgaa
    15601 caggtcgccg cccttgatga tgcatacaag ccccgccgca aacagaagga cgatccaaag
    15661 cggggtactg ccggtgaaat tcaggctcat aacaagctcc tctttgggat gaaatcatgc
    15721 aaaaaaagag actttccgca cgatcatcct atgtgatgtg ctaaaagtct cattacctgt
    15781 caatcaggcg catggccacc ggcggaggcc gggagatgtt ggccatgcct tgacaactac
    15841 tcccttttaa cggaaggtag catagcacaa caaaagaagg aagtcaatcc ggaaatcccg
    15901 ctgaaaaagc aggggcaaaa gcggccccga agggaagaaa acccatggtt ttcctctcct
    15961 cggggccgca gggcaacggg cgcggtcgta agccgagttc tgtcgttgga cgatcatcta
    16021 tctactcccc gcgtcgccgc gaggctctag cgatctcttt agaagcacga cgagcagccg
    16081 tatcgcttct ggtcggatct tgcatcgggt ggggtttaca gcggaaccgt gtcgccacgg
    16141 cccgggtgag ctcttacctc gcctttccag ccttacccgg acaaaagccc gggcggtata
    16201 tctctgttgc actctccttg gagttgcctc caccagccgt tagctggcac cctgctctgt
    16261 gatgctcgga ctttcctcat ggtatcccac gcgatcgcct ggccgcccca ttgcccgtcc
    16321 agcatagcac gcccggcggg ggaagtcaag caggaggtct tattcccccg ccttttccgt
    16381 cagcacttcc agcgccgtca gctcgtggcc gtcctcgtcg gtgtattcgc cgctgaaata
    16441 gaccgtgacc cggtcgccca tggcggggcg ggtcgcagac tcggcggagt cttcctg
//
LOCUS       DAWAEA010000003        13565 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_707_length_13565_cov_2.521244, whole genome shotgun sequence.
ACCESSION   DAWAEA010000003 DAWAEA010000000
VERSION     DAWAEA010000003.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 13565)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13565)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13565
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_707_length_13565_cov_2.521244"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    585..677
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9684..9693
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10513..10522
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13277..13327
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2475 a         4244 c         4136 g         2546 t
ORIGIN      
        1 aggtgtatca gaatcaaggg gccagcaagc ccgccaaatt cctcggtaac atcaaggcca
       61 tgctcacgcc ctttgccgcc ctgcaggggc gcatcaccga ggtgacgacc agcgagggtc
      121 tgaaggaaaa agttggtctg aaaggcaacg ccggcgtgca gatggccttc aagggcggct
      181 atacctatcc cttcatgatc tgggccattc cctgcttctg cgcgatggac ttcaccttct
      241 cgctgggcac tgcgttcagc atcggcctta ccgccgactg gcccagcatg tccaatatgg
      301 cgctggactt cggcaccggc gtgaccatcg ccattctggc ggagctgggc gtcagcggcg
      361 gcgtgggtgt aaaggacttt ttcagcctcg ccgtccgttt ctacggcaac atcaagccgc
      421 ttttgcggct gggcaagagc agcggcgcgt ccgttaccct gggcttcggg ctggaggtca
      481 cggcgcagat gattctgctc aaattgaaaa ggacgctctg gaagggcagt tggagctctt
      541 cctctaatgg aacaagcgcc ctgaacgcgg cgaacggagc ggaannnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnctg atctggtata atctggacga ttcctcgcgg cacggcgaaa
      721 tttacccctc catgggcggc gacgcccagc ggagcgcatc gggcaccgcg tccgattacg
      781 ctttcgccat ccgaagccat caggatatgg tggcggtaaa cgtcctcagc ggcgtgttca
      841 ccgaggggca ggatcacccc tccaagagca ccatgacgct ggcggtgctg cagcttcaga
      901 acggctcgct ggtgatgaaa aaatacgcgc cccacgtgac ggaaacgccg aacgccgcgc
      961 ggtatttgtc ctccccgatc gtatacttcg tccggcacaa caacgccacc gcagctgccg
     1021 aacctgtctg gttcctgaac gcttcctgcg cccgggagga catcaagagc ggcgagatca
     1081 ccgccgtcca ttccatggat ctggatcaga ccagcaccgc ttatcaggtc acgatcgacc
     1141 ccgtgcccga cggcatcgaa aacgtgcagg gtgtgcgccg gatgaccccc gccacgccca
     1201 ccaacgccac cttcaacggc gattatatca ccggctccaa tggctccaac cccagccttt
     1261 cctgctacta ctggctgaac gagaagcagc agctttccgt ccacctgaac aagaccaaac
     1321 gggttctgga cgaacacgtg gtcttcatgg aaaacatggt ggaatatgcc gtgaacgatg
     1381 cgcgggagtt cgttttctac ctgaaggaag gcttcgcgga ggacggctcg gagtgctacc
     1441 ggctgatggg cgcgacccgt cagggaaaca aggaatttac cgtgcgggat tacgacgtgt
     1501 gcctgtacgc ccagcgtttc cagaccgccc acgtgaacga cggctcccgg tacggcattc
     1561 actacctgta ctggacggag agcatcgccc ccgaccccga caacgccgat caggaagaac
     1621 gctaccgcgt ccggtgcgtg cgcttcgacc ggcccaccaa tacccgggcc accaatacca
     1681 tgagcgcacc cttcacgctg gtggagctgt cccactaccc ggccagcatt cacctgctgc
     1741 cggacggtac cggctattac accaccgatc tggaacaggc ccaaacgctg aatgaatccg
     1801 ccgtgaacag ccgccggctg atccgctttg agttcgagtt gaagatcgcc gccgagctta
     1861 ccggcgtggc ttccagcgac ccctgtgtga ccgccggcga aagcgccggg ctgctcttct
     1921 ccgtggaaaa caagggcaac ctgcccatct cccgcttcgg cgtcaccatc aagcagggca
     1981 gcaaagtcgt tcagcagatt ctggtggact gccagaatcc ccagaacagt gtgaacagcc
     2041 tgtttgcctc caacggcccc ggctattccg ttacccggct ggaaaacgtc tatgacgata
     2101 taaacggcga ccgttggctg gtgaccacta ccggcgacaa cggtcagatg gtagaggaag
     2161 cgctccatac cgaccttctg atgcccggcg gcgttcatac ctatgaagcg gccatcgaaa
     2221 ttcccgacga ttgggacgga accattgcgc tgaccgccga gctggaagcc gtctacgcca
     2281 tgacccagta tgcctcgctg atgcagggaa gcgccgtcac gcaagccaac gcggcggggg
     2341 acacggctct gtacgaggtc tgcgccctgc ccacgggcga agtgaccggc tatgccatga
     2401 ccaacgcgcc cggccagatc ggcgttggcc gggccaatac gctgcccgaa gacacctcga
     2461 aggagatcgg tgtgggccag ggcgacctga tgctggactg ccagccctat gtggatgcgg
     2521 acggcgagga atatgtgcgg gtcagcatcg tggggcgctc ccagacggac agcaccatcg
     2581 cgccgaccct gaccgccacg atggacggca aaatcgtctt cagtcacacc ttcatgcggg
     2641 ccatcgaccg ggacttcggc tatacgctgg acttgcccgc cgaattgctg ctggccggaa
     2701 aggacagcgg cgacgtgacc ttcctgctga ccgacaacga ggagggcaac gagttttcag
     2761 actttgataa cgagcgcacc gtatctctgg gcaacgagct gcgcttcctg aagcagcctg
     2821 aatccctgtc tcggacggaa ggggaagaag ccgtgttcag cgtggcgctg gccgggggca
     2881 agcagcccta cgcctaccgc tggcagcgga tggaccccaa cggcaaatgg acggacatcc
     2941 ccggagccac tcaggccgag taccgcattg ccgccgttca ggcgaaggac aatggcgctc
     3001 tgttccgttg catcgtgcag gatcaggatg gatgctctct gacctccgag gccgcttcgc
     3061 tggccgtgtt cagtctgcca cccaccaccg gcgactcggc tcaacccgtg ctgtgggcgc
     3121 tgcttctgct ggtgtgcgcc gccggttggc tgctgtgccg caggaagaag gcctgatttc
     3181 ggacgaacgg cccttccttt ggaaggaaaa tccgccctcc gggacgaaca taataggcgc
     3241 agggcgttgg ctctgcgcct tttcgcagtc attcagccaa agaaaggatc caagcattat
     3301 gagactggat aaatttttga aggtatcccg catcatcaag cggcgcacgg tggccaacga
     3361 ggcctgctcc ggcggtcgcg tcagcatcaa cggcaaggta gccaagcccg ccgccgacgt
     3421 aaaggaagga gacgagctgg aaattcgctt cggcaaccgg gtaggcaagt ataagatcct
     3481 gagcgttaag gagaccgtcc gcaaagaaaa cgcgcaggat atgtatcagg tgctggaaga
     3541 ggacgcgcag accaaagccg aacggagcgt gggcgaatga aggtctgcgc cgtgatcttg
     3601 ggcgggggca gcggctcccg catgggcgcg aagcagaata aaatttttct gccgctgcgg
     3661 ggcgtgcccg ctatcgtgcg ggcagcagct ccctttaccg gcctttgcgc ggaggccgtc
     3721 gtggtggccc ggccggagga acgggacgaa atgaccgccc ttctgcgccg ctacgggctg
     3781 gattccttcg tgaagaaggt cgtgccaggc ggcagcaccc ggcaggagtc cgtccgccat
     3841 gggctggacg cgctgccgga cgacacggac attgtgatga tccatgatgg agcccgggcg
     3901 ctggtgacgg aggacgtgat ccggcgggcg ctgcagtccg ctgtggagcg tggctccggc
     3961 gtggcggcca ttcccgtgac ggacaccatc aagcgggcag gcgatgacgg gcgggcgctg
     4021 gagaccctga accgggacga gctgcgggcc atgcagaccc cgcaggcctt tcagctgccg
     4081 ctgcttcgcc gtgcccacga attggcggag caggacggct ttctgggcac cgacgacgct
     4141 gccctgctgg aacacgccgg tctgcccgtc tacctgacgc tgggggacaa agaaaacctg
     4201 aaactgacca ccccggcgga catggccttc ggcgaggcca ttctgaccct tcgccgggaa
     4261 aaggaggcgc tggcatgatc cgcatcgggc atggcatgga cgtacaccgg ctggtggaag
     4321 gccgaaaatt gatcctctgc ggagtggaaa tcccccacac gctggggctg ctgggacaca
     4381 gcgacgcgga cgtggcggct cacgctctga tggacgcgct gctgggcgcg gcggcgctgg
     4441 gcgacatcgg caagctcttt cccgatacgg acgagcgcta ccggggcgcg gactccctca
     4501 tgctgctgga tcgggtgatg gaggcgttgg gcgaggcggg ctaccgtccc gtcaacgcgg
     4561 acgtgaccat cgtggcgcaa aagcccaagc tgcggcccta tatcgaccag atgcgggtcc
     4621 atctggcccg gcacatgggc gtggacgtat cgcaggtcag cgtgaaggcc accacctccg
     4681 aaggcatggg ctttgagggc gaggaaaagg gcatgacggc ccacgccgtg tgcctgatcg
     4741 aatccatctg aatttccaat gaaatcaagt gcaaaagctg accccatggg gtcgtttggc
     4801 atcacgccca tggagcgcaa tcgctggatg ctggcgctgg gcccgtcctt ctgggcgggc
     4861 tgttttgccg cgccgctttt ccagccgggc gcatggccgt gggcgctggc tttggcgggc
     4921 gtggggctgt ttttgcttgc gcgctttggc gggcgaaagg gcttcgccgg agccatgctg
     4981 tgcgcggcgg cgctggggct gctgtggacg acgccccgtc tgacgcccgc cgcgccttcg
     5041 ccgggaacgt atggggagat caccgggttc gtttacggcg aaccaaagca gcgttcggac
     5101 gaacgcctga cctttaccct gacggatatt cggctggatg gtgaacgctt ttccggccgg
     5161 gcctactgct ccctgtatta cgagggagag gagccgccgg agctgtacga cggagcggag
     5221 ctgcgctttg aggggcgagt ctatcagcca gacggcaaaa gcggcgctcc ccggttcgac
     5281 ttccgtcaat ggatgctgaa aaacggaatg cccttcggca tcaccatttc caagggcgtg
     5341 gaggtacgaa acaccccgga gagtgcccgt ccgcaggatg cggcggagcg ggtgcggcgc
     5401 agcctgaacg ctgccctgaa ccgggtcatg ggcgaggaag cgggcgttgc ctccgcgatg
     5461 ctctttaacc acaaggaagc catgagcgaa gatgagaccg ccgcctttcg gacgctgggc
     5521 atcgcccacg tcacggcggt gtccggcctg cacgtgggca ttctggcggc ggcgctgctg
     5581 ccgctcctcc gccgtttccg ggcgggaaga aaagcccggt ttgtgctgct ggccctgttc
     5641 ctgttggctt atagctggct gaccggcttt tccgccgcca gtcttcgtgc gtcggtcatg
     5701 ttcctgctgg ggcttctggc gcagggctgg aatcgaaagc cggagttcgt tctctctttt
     5761 tccgccatgg gcgcgattct cctgctggcc cccggttggc agcgctgggc ggaacgccgc
     5821 tggccggaag cccacggacg ttttcgcggg caaaagctgc gcgcccggct gtcccatctg
     5881 gcccatcagg ccaagcgggg actgtgcgtc tcgctggcgg cgcagttggg cgtactgctg
     5941 cccacagcgc tatattttca tcagctgccc ctgtacggcg tagcggtcaa cgtcctgctg
     6001 ctgccattga tcgggctgct ggtgcccctc tatgccgtca cgctgctttg ctcgtggatc
     6061 cccgggctgg gcactgccgt aggctttgcc gccgctctgc tgacgaaggg ctttaccggc
     6121 atggtttccc tgctctccca gctgccctat gcgtctgtcc gtgtagcgga gccgtcggcc
     6181 atctggctga tggctgcacc gcttctgctg atcttccttt cccctcgagt gtggctgaag
     6241 ccgctttccc gctgtgcggc aattttgttg actgtgctga ttgccgtagg cggcacatgg
     6301 ctgacccgtc cccctgagac ccgctatgtt cagctgtccg tcggacaggc cgacgcggcg
     6361 ctgctttttg acggggacaa gactgtcggg atcgacatcg cttcggacgg ttccgccgtg
     6421 atcgactatc tgctggccga agggcgcgat ctggacgcac tgtatctgac ccacctgcat
     6481 ctggatcacg ccggagggct aagcgaaatt cttaacgcgg gcattcagat tcgccaaatc
     6541 tatgtgccgt ggaacgcgga ggagcagcgg ctggacgagc gctcgctggc cattctggcg
     6601 ctggcccggg aacgcggcat tcccattacg tcccttgcgc ggggcgacga gctgcgctac
     6661 aataagaccg ccattcaggt gaaatggcct gaccgggact gccgtcgggg ccagaacgcc
     6721 aacgatctgc cgctggtgct ccgcatcgat ctggacggcg cgctcatcct caacaccagc
     6781 gacctgaccg gctcctatga gcgctacgcc gccgcgccct gcgacgtgct gaaagtggct
     6841 catcacggca gccgggattc cagcggcgag gaatttctcg cttttgtcag ccccaagctg
     6901 gccctcatca gctgcagctc gaccagccgt tccctgccgg gcgcggaaac gctggaacgt
     6961 ttggccgcgc agaacattcc cgtactgcgc accgaccaga gcggcgacgt gaccctcttt
     7021 gtggaaaacg gccggctgca ggtgcggccc tatcgcttac gatccttacc gggaggcatt
     7081 gaatgaacca taccgcgttt tttgagcagg tcaaaaaggg aaacatccag cccgtctacc
     7141 tgatggaggg cacggaggaa tacatcaagc agcaggcggt gcgccagctg tgcacgaagc
     7201 tgctgcctgc cgggctggag gagctgaacc tgactgacct gaccgacccg gaggccgacg
     7261 cgctcatcgc cgccgccgaa accctgccct tcatggcgga caggcgcatc gtcatcatcc
     7321 gggaatgcag ccttctgacc gccgggaaaa aggcggaaaa cgaggacaaa gccgctcaga
     7381 tcgccgaata cgtggagcgt atcccgccca ccgcctgcgt ggtgtttttt gtcaagggca
     7441 aggccgacgg acgcaagaag ctgtacacac tgctgaaaaa gaaaaacgcc attgtggatt
     7501 tctcccccat gagcgacgcg gaatgcgcgg actgggcccg gagaaccatg gcgcggatgg
     7561 gcaagcagat gacgcctcgg acggcggaca agctggtctt caccgtaggc cgggacgcgg
     7621 cgctgctcaa gcaggaaatg gaaaagctgg ccgcctatct ggccgaccgg ccagaagtga
     7681 cggaagcgga catcgacgcg atctgcaccc gttccaccga atgcacggta ttccagatgg
     7741 tggacgcgca ggtggcgggc cgcaacgacg tggccttcgg ccttttgaag gacatggtgc
     7801 gggccgggga ggatcgcatc ggcattctgg ccatgctgct gcgtcaatac cgcattctgt
     7861 accacatgcg ctgcctgatg gacgagcgcg caccccagca gagtcaggct tcgctgctgg
     7921 gcatcccgcc ctttgccgtc agccgcaccc agcagcaggc ccgccgctac gaccgggccc
     7981 ggctcaagga ggcctatgag agcctgctcg actacgagca gcagatcaag ggcggcctga
     8041 ccccgcagga gggctgcgcc gaaaatgctc tgctgcggct ggaagccatt ttgcgggcat
     8101 aaaaacaata gaaaaaagag acgcatggga agaaaaccct gcgtctcttt tttaggttat
     8161 caaaattttc agccgcgaac agcggtcagt ggtgcaaatt tcgagcttgt gccgtttgaa
     8221 gccccttcca tcggcagaat gcctttggga aacccatgct ggacgcttcg cgcccttttt
     8281 gcggctcgct tttcggagtg taacttcggg tctgctgccc atcttcgcgg caatccccat
     8341 tctcgatgct ttgcacgctt ttacggtctg gctctctgtt tggcatatgt cttggacgac
     8401 ggcaaggttt cgtctgccca cgttctcagg gcttcgtatc cttgtgcagc ccgtcccttg
     8461 tggggcacgg cttcgggtct gtaccgcttc atagtgctct caaccctcga tgctgcgcgc
     8521 tctccttcca ctgtggccat ggcttctcca aagctcccct tgcggggcat ccgccttccc
     8581 cctttgccgg aaccttccga atcgctgtcc ggccttttcc ctcgttttcc ttacagctta
     8641 ccttgacgga accgctcgat cagttcctgt gcgacgctaa aggtactggc aggggcggaa
     8701 agctggccac gggacaccca tttggcgtca gccagctcgc tccgctgaat cttcaagggc
     8761 agcgagctgt cagtctcccc ctgaaaggcg cacatcagcg acccgctgac gccccacggc
     8821 tggctgccta cataccgcaa gtgatgaacg ggcagtccga cctcctccat cacttcccgg
     8881 cggacggcct gttccagcgt ttcgcccact tccacataac ctgctaccag cccgtaatgt
     8941 ttccacgggc tgttggcact cttcgccagc aggatagcgt cgccgcaggt cagtgccacg
     9001 atgatcgccg gggcgatcat gggatagttc acctgcccgc aggcggggca gcgcagggcc
     9061 cgttccgccc cgtcgtgctc catccggccg ccgcagccgc cgcaaaagcg attccggcgc
     9121 atccactgcc acagatggtg ggcggcggtc agcacagtgc cggagcggca gtccatttcc
     9181 cgaaacagcc gcatcggccc ataggacagg ccgtttcctt gggggatgac ctcgtccccc
     9241 agcggatcgg gcgtaaagac cgcctgtccg tccgccgtca gcagcgcgat caggacggtt
     9301 ccctccggca acaggggttt gacctgccgg tagaccggca gacagggcac gctctctttt
     9361 tcaaccaaca gcacctgtcc cggctgaaaa cacagcaccg ggctttcgtc cttcggggcg
     9421 gcggcgcagg gctgcaccgc gatcgtcacg tcctcaaaca gcatcgtatc tccttctttc
     9481 tgaacccatt acaggaacct tgccgttacg ctttcctgta cctgccgcac ttcctccggt
     9541 gtgccgacgg ccacgatccg tccgccttct tccccgccgc cggggcccat gtcgatcagg
     9601 taatcggcac tgcggatcac gtccagatca tgctcgatga cgaccaccgt cgcgccgtgg
     9661 tccagcagcg cctgaaagac ccgnnnnnnn nnngaccgat ggtgggctcg tcaaagatga
     9721 aaacggaatc cgactgggtc ttgcccattt cctcggccaa tttcagccgt tgagcctcgc
     9781 cgccggaaag actgggcgtt tcttcaccca gcgtcagata gccaagcccc agttccttga
     9841 gtacctgcag ccgttgccgg atcagcttca gcccgtcgca ggcttccagc gccgtgttca
     9901 cgtccatgtc catccagtcc ggcagggtat ggtctccgtg ccgcaccagc tgggcttccc
     9961 ggctgtaacg gcttccccgg cattccgggc agggaatttc cacatccggg aggaactgca
    10021 cgtccagact aatgatcccc gtgccgtcgc agaccgggca gcggagcttg ccggtgttgt
    10081 aggaaaaatc cccggcctta tacccaagcg tcttcgcgtc cgccgtcctg gcaaaaacct
    10141 tccgcagctc gtcatggacg ttggcataag tggccacggt ggagcgcacg ttgatgccga
    10201 tgggggctgc gtcgatcagc ttcacttgcc gaacgccctc cgcctccact ccggccacgt
    10261 gttccggcag cttttcgccg tgaatggcgg cgttcagccc gggcgcaagg ctttccagca
    10321 ccagcgtggt cttgccggag ccggacacgc ccgtcaccac cgtcagccgc cccttgggca
    10381 tacggacttc cagcggcttc accgtatgga tggcccgggt agacaagcga atcgtcccct
    10441 gcgagaaaag ctcctccacc gggcagtgcg tccgtgccac ctgcgccgtt cccgccagaa
    10501 acggcccgat acnnnnnnnn nngaatcttc agcaagcgcc gcgggcgtac cctgagcgat
    10561 cacccggccg ccgttggctc ccgcctccgg ccccatctca atcatccaat tcgcctccga
    10621 aagcagctgc gtgtcatgat cgaccagcac cacggagttg ccgtcggcga tcaagtcctg
    10681 catcacaccg gtcaagcccg ccagattggc cggatgcagc ccgatggagg gctcgtccag
    10741 cacgtatagc acgccggtgg tacggttgcg cacggcgcgg gccagctgca tccgctggcg
    10801 ctcgccggtg gacagagtcg ctgccgcccg atccagcgtc aggtacccca gtcccagctc
    10861 cagcagccgc cgggccgtca ggtgaaagga gtcgcagatg ctttgcgcca tgggtctcat
    10921 gctttccggc agggaagcgg gcacatcggc cacccactga ttcagctggt tcagcggcat
    10981 ttggcagacc tccgccagcg tcagcccccg catcttcggg gcgcgggcct tttccgacag
    11041 gcgggtgccg ccgcattccg ggcaggtctc ctgctttagg aaacgctcca cccgcttcat
    11101 gccgctttcg tccttcacct tggccagagc gttttccacc gtgtagacgg cgctgtaata
    11161 ggtgaaatcc agctccgccg cgtcgttgga attttttggt ttatagaaaa tgtgcttttt
    11221 caccgccggg ccgtgataga cgatctcctt ttcctccgct gtcagctccc gaaagggcac
    11281 gtctgtccgc acgcccatgg cgcggcacac gtcggtcatc agcgaccaca tcagcgtttt
    11341 ccacggggcg accgcccctt cgtcgatggt caggctgtcg tccggcacca gcgacgccat
    11401 gtccaccgtg cgcaccgtgc ccgtgccgtc gcaggaggga cacgcgccct gcgaattgaa
    11461 ggacaattct tccgcggacg gggcgtaaaa ctttgcgccg cattccgggc agcaaagctc
    11521 gtgcccggcg gctacctcca aggtgggcgg cagataatgg ccgttggggc agcggtgact
    11581 ggccaagcgg gagaacatca gccgaaggct gttcagcagc tccgttcccg tgccgaaggt
    11641 gctgcggatg ccgggcacgc cgggccgctg gtgcagcgcc agcgcggcgg ggacgtacag
    11701 cacctcgtcc acctgcgcct gtgcggcctg cgtcatgcgc cgccgggtat aggtggacag
    11761 ggattccaga taccgccggg agccttcggc atacagcacg cccagcgcca gcgaggactt
    11821 cccggagccg gagaccccgg caatgcccac gatctggttc agcggtacgt ctacatcgac
    11881 gtttttcaaa ttatggaccc gcgcgccgcg gatctgaata tgatcgatca tcggtttctc
    11941 ctatgccgtt ttcggcggtg atggttcggc gcttccgcgc cgttccctca ctatagcaca
    12001 ggaaagggca aatgaaaacc cgccgcgtat cgaagcggcg ggacagtttc cttattcgtc
    12061 caaaattcga ccatcggtag aaatgggcat aagcataggc cgtacaatcg gcggcaatca
    12121 gcagtttggc cccatcgaaa tagggcgcat tcaccggcac cagacggatc tgcaccggcc
    12181 actgggaaag ctggctgggc atctcctgcg cagcggcggg cgcgggggca ttttcccggc
    12241 gaatggcccg ggccatcatg ccggggcagc cgccgtgaac gggtttgacg tgctccagcg
    12301 ccttttccgc ccgctccttc aggtgctttt ccaccgccgc ctcgtcataa gcggccgctt
    12361 cccgctccac aaaggtaatt gcgcccgtgg ggcaggcggg caggcagttg cccagtccgt
    12421 cgcagtaatc gtcccgcatc agccgggcct tgccatccac catggcgatg gccccttcct
    12481 gacaggcgga cgcacacgcg ccgcagccgt tgcatttttc ctcatcaata tggataattt
    12541 tgcgaatcat attcttccct cccaaaagtt cggtcgcttc ggggcaaggc ccctgcggcc
    12601 ccgcaaagcc gattcttcgg gagcgttatc cccgcttgac tttgcacgtt cagtctacta
    12661 caattcagct ggattgtctg ttgaattttc aacgaaagga aaaagcccat gcgagaaatt
    12721 ttaccgctgc ttcgttccac atccctgttt acggggctga cggaagagga aactgaatcc
    12781 cttctttcgg cgctgcacgg agaaatgcgt gcttttgacc gggaggagtt actgttgcgg
    12841 gcaggacaac ccgtccgggc cttcggggtg ctcttaagcg gcggcgcgct ggtgcggcag
    12901 gaagatttct ggggcaacca gaacatcctg tcctctctgc atccggggga ctgctttgcc
    12961 gagactttcg cctgtctgcc cggcgcggtg ctgaacgtgg acgtagcggc cagtcagccc
    13021 tgcgaggcgc tatttttgga aacgcagggg cttttgaccg gcggtctttc cgcccatgcg
    13081 ggcggagcgc ggtttttaca gaattttctg gtgaatctgg cgggaaaaaa tctccggctg
    13141 aacgagaaga tcacccacat ggggcagcgc agcacccggg cgaaggtact ttcctacctg
    13201 tcggcctgcg cccggcggca gggcggcgct gaatttgaca ttgccttttc acggcagcaa
    13261 ttagcggact atttatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13321 nnnnnnngat cgaagcggct tatcggcgga gctttgtcat ctgcgggacg aagggttatt
    13381 ggagttccac agggagcatt ttcggctgat aaagccgttg gaggaaggat gggagtaaag
    13441 gggatagggg ggattggcgc gccgactacg gcgagggagt gggatttgac gtcccatggg
    13501 tttcttgcca actttggcgt ccgaaggttt ccaaagggga acgaagggaa gcgggtatcc
    13561 agtgg
//
LOCUS       DAWAEA010000004        13344 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_724_length_13344_cov_2.647679, whole genome shotgun sequence.
ACCESSION   DAWAEA010000004 DAWAEA010000000
VERSION     DAWAEA010000004.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 13344)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13344)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13344
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_724_length_13344_cov_2.647679"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    10297..10392
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11851..11860
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2715 a         3896 c         4017 g         2610 t
ORIGIN      
        1 gctcgcgggt ctggtgtggt tttctggccc gtgcagggaa aaaacaggcg gcgcgtcgaa
       61 tttccgcttg cggaatgaga cgaatttcgc tatactctgg ggaaccgtgc acagcggttc
      121 cccggaaagg gcggaggcgc gggcttctgc caaagaactc ggggctggaa aggctccggg
      181 cgttttttga tttagggtga aggaagcgaa agatgatgaa gaagagttcg cgcatggcgc
      241 tggcggcgct ggtggtattt ctgttctgcc tgtaccggga cagccgggga aagggcgacc
      301 cggaaacggg cgacggcgtg cccttccggc agatgacggc ctcgcagaaa aagacgctgc
      361 tgctgggcgg cgccttctgc agtctggcgc tggccgtcgc cagcgcactg cagcagatcg
      421 gcatcgagca gggcacgtcc gccgggaaag ccgggtttat cacggccatg tacattcttt
      481 ttgtgccgct gggagaactg ctgatcttcc gcaaaaagct gcgctggctg ctgctgcctg
      541 cggtgctgct gagtcacggg cttatacctg ctgtgcatga aggcgggcgc ggcgctgacg
      601 ctggagggcg gcgacggcac gctgcttctg tgcgccgtgg cgtttactgc ccatattctt
      661 atcattgacc gggtcagccc ccgcaccgac ggcgtgaagc tgagctgcgt tcagttcgcc
      721 gtggcggcgg tagtcagcct gatcgtggcg ctgatcgcgg aaaagccctc ctgggacgag
      781 atcatccgct gctgggtgcc tattgcctat gcgggcgtca tgagcggcgg cgtgggctac
      841 accctgcaga tcgtgggtca gaaggatacc gaccccaccg tggcctccct gatcctgtgc
      901 ttggaaagcg tgtttgcggt gctgggcggc tggctgattt tgggggataa aatgacagcc
      961 cgcgaatacg cgggctgcgg catgatgctt atcgggattt tgctggctca gtggccggag
     1021 aagaagccgg cggcttcggg atccatccga ccgtaaagcg gaagatcacg aacaccagcg
     1081 tgctcaggaa ccaggtgatt gggtagacga agaccacctg gcgcaggtcg gtgaacgacg
     1141 ccgagaacag ggagatgtag atcacccgca ccagacacca gcagacggcg atcacgacca
     1201 tggggacgac ggccttgccg gtaccgcgca gaacgctggc cacggcgtgg gagaacgcca
     1261 tcaggaagta aaacggcgtg accgtgcggg catatcgaat gccgaaggcg atcatgtccg
     1321 ggtcgccgcc gaacagggac agaagctgcg gggccagcag gtagatcagc agcgccacgc
     1381 cctcggccat caccatgcag gagatcacac cgaagcgcgc cccctccttg gcacggccat
     1441 attccttcgc ccccaaattc tggctgacga aggtggtcag ggccagcacg aacgcgttga
     1501 tgggcaggaa gccgaaaccc tcgatcttga ggtaggctcc cgcacctgcg atagccgtcg
     1561 caccgaacag gttgatgccg gactgcacaa aaatattgcc aatggaaata atggaatttt
     1621 gcagacccgc cggaatgccc atatgcagca ctttcgccag catggggccg ttcaggcgga
     1681 cttttttcgg ctccacccgg taaatggcgt cggagcggat cagacggatg aagcccagca
     1741 ccgcgctgac cgcctgagag atcaccgtcg ccagcgccgc accgtccacg cccatgtgga
     1801 acacggccac gaacagaagg tccagcacca cgttggtgac ggaggaaata atgaggtaat
     1861 acagcggatg cctgctgtcg cccacggcct gcagaatgcc ggtggcggtg ttgtacaggg
     1921 taacgaacac cacgccggag aaatagatgc ggaagtacag cgccgaattg gcaaagacgc
     1981 tgtccggcgt gccgatccac cgaagcaggg tgggggacac ggccacgccc accacggaca
     2041 gcaccacgcc cgccagcagg ccgaaggcca cggtggtgtg gatggcggtg tgcatcttct
     2101 gatgctcctg cgcgccgaag tagccggaaa tgaccacgct cgcgccggtg aacaggccgc
     2161 tgaacaggcc taccagcagg aaaatgatat ggccggagga acctaccgcg gccagcgcgt
     2221 cgctgcccag aaagttgccc acgaccagag agtccacaat gttataaagc tgctgaaaca
     2281 ggtttcccca aaaaacagga agggcgaaac gcacgatttg ccggcggacg gagccgccgg
     2341 tcatgagcgt tacgccctga gaatgttcct cattattttc ccgggtgtgc ataggcgatg
     2401 atcatctccg caatttcttc aggggtgtgg tcgtcggtgc gcagcatcag gtcgtagttg
     2461 ctccgaacgc cccagttgcc atcggcaaag aaattatagt agctcttgcg ctgcttgtca
     2521 gtcttgcgga tcagttcccg ggccttgtct gcatccacgt gctccagccg catgatgcgc
     2581 tccacccgct tttccagcga ggcatagagg aagatgtggg tcacatgatc catatcccgc
     2641 agcacatagt cggcgcagcg gcccacgatg acgcaggact gctccttggc cagattggtg
     2701 atgacctcga actgggccag aaagacccgc tggttcatgg gcatctgcag cccgagcccc
     2761 acgcccgcgc cgaagcccgt gccagccgtg accggcagca tgtcggacac ggtggtcttt
     2821 tcatcgttgt gctcgaaaat ctcctgacaa atgccgctct tctgagccgc cagggtcaaa
     2881 agctccttat cataaaacgg aacgttcaat ttctgcgcga ccaatttggc aattgcccgg
     2941 ccgccgcttc cgtattcccg tcccacggtg atgacgaaat gttccttcat agccttttcg
     3001 ctcccaactg tggcatggtt tttgcagttt tcctttccac agtcccatta tacaacaccg
     3061 gcctcaaaag ttcaaacccc caaagaggag aaattcattc cgaaacgccc ggacgggcat
     3121 ggctgcttgc gtctgcggcc gcgtccgaat ataataagaa aagaagcgag aacacggttc
     3181 tttcggggaa gggggaaggg cgatggaatt tgtttccgtc cgggccacgg cggagcgctg
     3241 gggcgtttcc gtgcgcaacg tgcagcggct tttgaaggag gaacgcatcc atggcgcgta
     3301 caagtgcagc ggctgctgga tgatcccggc ggacgcgccc aagccggacg atccgcgccg
     3361 ggagcgcaaa aaggcccggg aagcaaaaac ggcggatgct gcggctccgg cggcggaaac
     3421 ggaaacgctt cggccacggc ggctggggta tggccttttg tccgtcatca tccccatgcc
     3481 ccggcgcgac gcggatgcgg tgctgcccct gctgaaggaa accgccctgc ggcgcgaata
     3541 cgaaatggag ctggcctacc tgcggggcaa ctacgagctg gccgcccgcc gcttcggcga
     3601 aacgcagccc gaggagccga cacggttctg cgccgccatg ctgacgatgc tggtctgcat
     3661 ccatatcaat aacggggatc tgtatgcccg agtagcccat gagatggaac aggaaatgct
     3721 catcggggac aaggactccg cccatatggc ggaggtggcg ctgacctatc cgtccctgag
     3781 catgaaggat atgggccggg tatcgcaatg ggtgcagaag ggcgactttt cccggctgct
     3841 gccggaagcc cggcccatgg gcatgtacct gagcctgaaa aatctgcagg gtcagcggca
     3901 ctacgccgag ctgctgtgcg cctgcaaggc ggcgatcgcc ctttgggaac gggaggacac
     3961 cttcaccatt ctggatattt atctgcggat gctctgcgcc acggcctgct acgagctgga
     4021 ccgggtggat gaatgcgacg atcatttgct ggaggccatg aagatcgccc tgcccaacgg
     4081 gttcgtcacg ccgtttgtcc agtcctggat gaccacgggc tgcgagatcg agcatctgct
     4141 ggagcagcgc tatccccagt ggagagaccc ggtggaacgg gtcagcatgg ccacatggaa
     4201 aaactggatc gctttccaca accgctacac ccgggagaat attactacgc tgcttaccca
     4261 gcgggaatac cgggtggcgc agatgatcgt ggccggaagc acctaccaga atgtggcgga
     4321 cgaattgtat ttgtccgtcg gcacggtgaa aaagctggtt tcatcccttt atgacaagct
     4381 gatgatccac tcccgggcgg aattagccga tatgatcatg taagcggtca aaatctgacc
     4441 gggatttgac ctggccgccc tctggcagtc cgccccgctt tatgctatac tgaaagacgc
     4501 cggcagcggc cggacggaaa ggagaacccc catgagcctg aatgaaacgg cccatcccta
     4561 caccatggaa gccctgaggg ccaaaaaagg atacattatt gatatggacg gcgtgatcta
     4621 ccatggcaac atgctgctgc ccggggtgga gcgctttgtg gattttctga aaaagaacga
     4681 taaacgattt ctattcctga ccaactccag cgagcgcgct cctcgggagc tgcagcagaa
     4741 gctggcccgg ctggggctgg acgtggacga gagccacttc tacaccagcg ccatgagcac
     4801 cgcggccttc ctcaagggcc agtgccctgg cggcagcgtg tacgctatcg gcgagccggg
     4861 cctgttcggc gcgctgtacg acgcgggctt caccatgaac gaggtcaacc cggactatgt
     4921 ggtgctgggg gagacccata cctacaatta cgaaaagatc gagcgggcgg tgcagctggt
     4981 gctgggcggc gcaaagctga tcggcaccaa tcccgacctg accggcccca tcgaaaacgg
     5041 catcgccccc gcctgcaagg cgctgatcgc ccccatcgag ctggccaccg gccacagcgc
     5101 ctattttctg ggcaagccca atccgctgat gatgcgccac ggcctgcgcc gtctgggcgt
     5161 cagcacggac gaggccgcca tcatcggcga ccggatggat accgacgtga tcgcgggcgt
     5221 ggaaagcggc atcgacacgg tgctggtgct ttccggcgtg acctcccggg aggaaatgag
     5281 caagttcccc taccggcccc gctatgttct caacggcgtg ggcgacgtgg caggctgacc
     5341 gattttcgcg gccttcgccg cagcggaaaa acaaaagggg aatgcaaatg cacggaattt
     5401 ctggcatttc ggaaaaaaca gcgcgcgtgt tgacggcgct gtttttcctg cttctggcgc
     5461 tggcgggctt cttgacggtg cgggattatg gtatgcccta cgatgaatac accgaaatgg
     5521 cgatcttcca cagcaacgtg gaggaatacc gcctgcattg gcccttcccc attaaggagg
     5581 gctatttcgc cagtgaagga ggtcagccca tttcccagag catcgaaaaa gatcacggca
     5641 tctgcgccta ttacccgctg attccgctgc tgccgtggct gcggcaggat atctacacct
     5701 attcgctggt gtggagcatc ctgaccgagt gctggttttt gctgggctgc atcgctgtgt
     5761 acggcgtgtg ccgcaatctg ggcatgggac ggctgatcgg ctgcgccgcc gcgctgctgc
     5821 tttatctctg tccccggttc tttgcggagg gacactacaa taataaggac gtagtgctgc
     5881 tgtgcctgtt cctgctgacc ttctggcagg gagcccggct gctccgcaag cccaccctgt
     5941 cccggggact gctgctgtcc tttttcgggg ccatggcgtt caataccaag atcgtgggca
     6001 ttttcgcgtg gggcctgatg ggccttgcaa cgatcgtttc cctgacggcc cggaagaaat
     6061 ggaaccgttc catggtaggg ctggccgctg tgaccatcct cagcttcgtt gggttttacg
     6121 tgctgctgac cccggccctc tggtcggacc ccatcggctt tttccactgg tgcatggaca
     6181 acgcggtgaa cttctcccgc ttcggcggcg aggtgctgtt ccggggcgcg cggttctggg
     6241 atatcaatca ggaaacgccc ctgccctggt actatctgcc cttcatgatc ctggtcacgg
     6301 tgccgctgcc cacactgttt ttggtggcgg cgggcagtgc ccgggcgctg gcgaagcctc
     6361 tgcggaagaa aacgcggaat ttgtccgatc cccggcaggt gctgggtctg gcctttctgg
     6421 cggcattcgc ggtgcccgtt ttgtacagct gcgtgggcaa gcccctgatg tacaacggct
     6481 ggcgacattt ctactttgtg tatgcgggca tggtgctgct ggcggccatg ggactggacg
     6541 gcattctgcg tgggctgcgg cagcggaagg cccttcgccg gacggcggcc gggctgacgg
     6601 cgggcttcct tgcgctgagc gccgtgggga tcgccctgaa ccatccctat gagtgcgcct
     6661 attataatgt gctgattccc cgagacgcta ccgagaccat ggaaatggac acgtggaacg
     6721 tgtgccccac cggcgcgtac cggcagctgc tgagccggac tcaggggcat gtgaccatcg
     6781 gctgctattt caacgacctg atcccggcgg cctacaaact gcccgccgac ctgcgggccc
     6841 ggctgaccgt cacccatgag gacgacgagc cgtaccttta ttatggggct acctatgcgt
     6901 gggtgtatca cgccgtgccg ccggcgggct accgggagct tttctccatc aaaagttacg
     6961 gaaataccgt gggaacggtc tatgagcggg tggaggaacc ctgagtgccg aactttattc
     7021 ctattttctc aaaaaacact ccgaattttg gagtgttttt ttcttgacat tcgtatcaac
     7081 ctctcctaca atgttacata cggtcagaaa gctggccaga actatataag gatgatacag
     7141 caagatggac gaacttcgat ttatccctat caataccgaa gacagcgacc ccggcgtatt
     7201 tgttcagggc attcacgtcc ctgcgcggct ggtagaagaa agcggtgtgt ggatccggga
     7261 ggcgggcagc gccattctga cccaggacca gtaccagatc gcgccttttt atcatacgct
     7321 gtggctggtc gctgacgagg gcgacatgac catgaccttc ggcaatcagc acctgcgctt
     7381 caagaagggc gaatgcttcg tcatcccggc gggcacgacg gactgccgtc tggaagaagc
     7441 ccgggacagc cgccttctgt ggttcacggt ggacggcgcg ctggccacgt ccttcctgcg
     7501 gcagatgaac gcccacaatc tggtgcccgc ccggcagggc atcctgccca atcaggtgct
     7561 gctgatccgg cagatcgtgc aggtgctggt gcgccacagc ggcaccgatc aggccagctt
     7621 ccagctggcg cagctgctct gggcgctgct ggcggcccat agcggccaca gcgtagccac
     7681 caacgcggtg ctgagccatg agatcgcccg ggtggtggac gccatgcggc aaagccagta
     7741 tcaggccaac ttcagcctgg cggaaatggc cgccatcagc cgtatgccca tggagacctt
     7801 ccgcaagcgc ttcaccacgg agctgggcat tcccccgctg ggctacctgc agttccagaa
     7861 gatggagcgt gccaagaccc tgctgcgggg cggcatgagc gtgcgccaga ccggcgtgga
     7921 gatcggtatg cccgacccct accatttctc caagcagttc aagcacatcg tgggcatgag
     7981 ccccaccgcc tacctcaagc acgtgggccc catggagccc gacggccggg cctgaccggg
     8041 gaaaaaagaa aagaaccttt tatttctgcg caagcctttg ctcaaaaagg cttgcgtatt
     8101 tttctggctg tgttatactg aaagacggaa ttttttccta cattctttct ttatgataag
     8161 gagattgcga tatgatccgg aagattggta tcctgaccag cggcggcgac agccccggaa
     8221 tgaacgcggc ggttgtgagc gtcgctcgtt gcgccgccat gaacggcatc cagctcatgg
     8281 gcatcaagcg cggctataac ggtctgctgg gcctgagcaa gaaccccgag gacgatatct
     8341 gcgccctcga tctggaaacc gtgctggata ttgccgacca gcgcggtacc ttcctgcgca
     8401 ccgcccgctg tgtggaattc aagcagcccg aagtgcgccg tcaggcggcg gagaacctgc
     8461 gccagctggg catcgacgcg ctggtggtca tcggcggcga cggcagcttc accggcgcta
     8521 tgtatctgtg cgagctgggt atcccctgcg tgggcatccc cggcaccatc gacaacgacc
     8581 ttggctacac cgaggccacc ctgggctatg acaccgctgt gaacgtgtgc gtggaagccg
     8641 tgcgctccat ccgcgccacc agccgcagcc atgaccgccc ccacgtggtg caggtgatgg
     8701 gccgcaactg cggcgatatc gccatgaaga ccgctatggc gaccggcgcg gaaatgctga
     8761 tcgttcccga agtggaatgg gatgtggacg aagtggccgc ccgcctgaac cgcctgatcg
     8821 agcagggcaa cacccgcgct acgctggtca tcagcgagca ctgctgggac aacatgaagc
     8881 ccttcgactg gcgcaagttc ctcaacgata acggcaagac cgtttatccc ggcgagccca
     8941 tcagcgctga gtatctggcc agcatcctca agcggaagtg cggcggcgcg gaggtgcgct
     9001 ccaccgtcat cggttacacc cagcgcggcg ctcagcccac cgcgcaggac agcgccttcg
     9061 ccttcgaggc cggccatcag gccgtgcagc tgctcaaccg cggcattgcc aatcaggcta
     9121 ttggcatccg tcacggccgc gtcttcaata tgcccatcat cgatgcgctg agcatgaaga
     9181 agaccttcga ccgcgaaatg tacaatctga tcaacaagct gtaagctgct tatggaaaac
     9241 cccgcctttt cccgtcatgg aaaagacggg gtttttcagc cgataggaaa ggtaattttg
     9301 tatgtttctt tccgattcgg cagagtgccg aaaaaccagt ccatttttct gcaaatttct
     9361 gcaaacgttc cctttttccg cgaaagcgac ttttcagaaa ttgatttttg cgctatacta
     9421 tgattgaata tacccgcgat acagcaaaaa ggagcgcgac ttatgacgga ttttgtaaac
     9481 aactggaata atgagcttct gacgactccc gtggctccgc tgggcctgat cgccatgccc
     9541 ggctgcgagg aaatgggcaa gctggtcaac ggctggctga tgaagtggca tgagcagcag
     9601 gaagccctgg aagacacgcc cctttactcc ctgatgggtt ccaatcggaa cgatttcctc
     9661 atcgaggccc gctgcccccg gttcggcacc ggcgagggca agggcatggt caaaagtact
     9721 gtacgcggat atgacattta tattctgacc gacgtgggag cctacaacaa aacctatcag
     9781 atgtacggcc gggaggtgcc catgtctccc gacgatcatt ttcaggatct caagcgcatc
     9841 atcggcgcta tcggcggccg cgccaagcgc atcaacgtca tcatgcccta cctgtacgag
     9901 gtccgacagc accgccgctc cacccgggaa agtttggact gcgctcaggc gctgcaggat
     9961 ctgcaaaatc tgggcgtgag caacttcatc accttcgacg ctcacgaccc ccgcattcag
    10021 aacgccgtgc ccctgatggg ctttgacagc gtccgtccca gctatcagat tttgaaggct
    10081 ctgttccgca aggataagga tctgcagctg gatcgggagc acatgatgat cgtcagcccc
    10141 gacgaaggcg cgctggatcg gaacatcttc ttctcctccg tgctggggct ggatatgggt
    10201 atgttctaca agcgccgcga ttacacccgc atcgtcaatg gccgcaaccc catcgtggct
    10261 cacgaatatc tgggagacga tgtggagggc aaggacnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10381 nnnnnnnnnn nnagctgaag aagcgtaagg ccaaccgcat tttcgcttcc gccaccttcg
    10441 ccctgttcac caacggtctg gacgccttca acaaggccta cgaggaaggc tacatcagcc
    10501 gcgtgatctc caccaacctg acctaccgca ccgacgaatt gaagaacgcc ccgtggttca
    10561 tcgaggcgga catgagcaag tatatttcct acatcattgc cagcctgaac cacgaccgca
    10621 gcctgagccg cctgctgaat ccctacagcc gcatcaagaa cctgatcgaa cggtatcacg
    10681 agcagcagac cgccgccggg ttccgtctgg tgtaagcgaa gagcatacat ggaacgacgc
    10741 tttttcggaa gcgtcatttt tttacttgac aattatatcg tcaatcgtta taattaaatt
    10801 atgatatatc gaacaacgat atatcgacgg gagagtgaaa cgaaccgatg aatgagaata
    10861 tggcgttgac ggaatccacc tactatatcc tgctggcgct ggcgcagccc cggcatggat
    10921 acggcatcat gcagcagacg gagcagctgt ccgggggacg ggtgcggctg gcggcgggaa
    10981 ccctgtacgg ggcgctgaac gccctgtgcg aaaagggctg gatcgtgccg gttcccgctc
    11041 aggaggaaag ccgccgcaag gcgtaccggc tgacgcagca gggcgaaagc gtcctgcggg
    11101 gcgaactgga acgcctgcgg gaactggtgc gcaacggaga aatcattctg gaacaggggg
    11161 cgaaggaata atggaacgca acgcggagac caaaaccgtt tttcgctggt ggtgggagtg
    11221 ggactttgac aaggaggagc agtggctgaa ccagatggct caatccggct ggctgctgga
    11281 taaggtaggg ttctgcattt atcgctttgt acgctgcgag cccggcgctt acagcgtccg
    11341 actggaaatg cacccctgcg ataaggatta tctttccttt atggaggaaa caggagcaga
    11401 gtatgtcggc cgtatgctgc aatgggtcta cttccgcaaa aagctgaccg acggcccctt
    11461 cgatctgttt tccgatatcg attcccgcat ccgtcatctg gaacgggtag ggaaccccct
    11521 gctggtgctg ggactggcga atttggccat cgggatcacc aattccattc aggccaacgc
    11581 cgaatatgca tggatcaatc tgctgtgcgc cacgctgctg atgtacggcc tgggccgcat
    11641 ccacggcaaa aaggaagcgt tggaaaaaga aagactgctg agagaataaa cagtcagagc
    11701 cgcatgagtt ttatcatgcg gctctcagac tgtcgaaaaa cccctgatcg accgcccgcg
    11761 cccgcgaaca gcgcttcgct ggttcgcgga aaaaatgctg cagcgtaaga cttaggggct
    11821 ccgcccctaa aaccccggca ggaggcactg nnnnnnnnnn cctccacttt tttgacacac
    11881 tgagagccgc atgagtttcg tcatgcggct ctgtttgtta ttcctgctga aacatccgtt
    11941 ccagctcgtc gtatttttcc cgggtaatga gcgcgtcctg cgggatgcct tccagccggg
    12001 cggcgtaggt ccagcggcca tagggagtga tatcccgaat cttgttcaga ttgacgatgt
    12061 agctcttgtg gcagcggaag aacaggtcgg ggggcagacg ctcctccatg tcgcccagcg
    12121 aatcgccggt gacgtaacgg ctgccgtctg ccgtgtacag cacggtggag cgttcctccc
    12181 gctggatcag cacgatatcc tcaaagctga ggaaactgac gccgccccgg tgatggagca
    12241 tcatgcggcc ctctacccgg ggccgggaaa gaggcggacg ggcaggctcc ggcgctgttt
    12301 ccggggatcg gagcaggttc cgcgcccgct ccagcgtctg catcacccgc tccaccttga
    12361 agggcttcac cagatagtcg aaggcgtata cctcgaaagc gtcgcccatg tagccctcgt
    12421 gggccgtggc gaagatcagc agaatggctg ggttcatgtc ctgaatgact cgggcgcatt
    12481 ccacgccggt catttcgggc atttctacat ccagaaaaac cacgtcaggc cgcagggtct
    12541 ccgccagctc gatggcctgt ttgccatcgg aggcttcccc ggccagcgtg aagccgtcgg
    12601 ccttttcgat cagccgccgc aggatcatcc gcattccgct gtcgtcgtcc gcgatcagta
    12661 cccgcagatc gccgttcatc cgcgccgccc ccatttccgc tggccgggcc gggtcagaat
    12721 ttcattcatg accacgccct gcacgatggc gcggctgtcc aacgacagca tgggggaggt
    12781 ttcctcatag aggttgggtt cgtccggcag gtgctgaacc gtaaattctt cctcatgacc
    12841 gtggcccagc tccgggtcgc acaggtcgcg gccctcgtcg ctgtccacgt tcaggctgcc
    12901 cacatagtcg ccgggccggg tcaggggagc ccgttccagc gaatgggaca ttccctgacc
    12961 ctcgtcggag aagccttccg cctcctggca gaagggctcc gggtcgaccg ttggggcggg
    13021 ggtaactctg gttacgctga cggggtctgt cggctcaaaa tgaatgggct tggggttcac
    13081 ctgtccccga cggtaagggg cggatttttt tcgggactgc tttacgccgc tctttcgaaa
    13141 cagcgatacg catacgccga tgataatcaa tgccactaac gtgtccatga gttccctcct
    13201 ttctgaaaaa cggtgagtcg gtcaggcatt acttgccgct tccttcgttc atccggggcg
    13261 cggcggcggg agcgctggcc ttggcgatgc cttcccgcat atcggtatcc gcttccagat
    13321 tcttcagctg atagtagtcc atta
//
LOCUS       DAWAEA010000005        13103 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_740_length_13103_cov_2.223099, whole genome shotgun sequence.
ACCESSION   DAWAEA010000005 DAWAEA010000000
VERSION     DAWAEA010000005.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 13103)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13103)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13103
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_740_length_13103_cov_2.223099"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1573..1582
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2830..2839
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7201..7210
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12223..12232
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2195 a         4220 c         3974 g         2674 t
ORIGIN      
        1 gcgtgctctt gcccacgccg gactgcccgg cgaaacagca gatgccctgc cgcatcacgg
       61 cccgcagttc gtccagcccc tcgcccgttt cggcgctgac ccggaggatg ggaacctgca
      121 gcggggtgta ttcccgcaca aggctttcat acacctccga cgaaaggtcg catttgttgg
      181 ctaccagcac cgggcggatg ctctgccggg ccgccatcac cagcagcgtg tcgatcagca
      241 gatagtccgg ctccggttgg ggagccaaca ccagcagcac cgtgcggata ttggccaccg
      301 gcggacggat cagttcactg tcccggggca gaatttcttc cacccagcca tgctcgtccc
      361 cgctggccgg ggtgaacaag atccgatccc ccaccatggg cgttacgtgc tgcttgcgga
      421 attttccctt ggcccggagc acgtgcactt ctccgtccgg ctgacgggcg tagtacaggc
      481 cgcccacgcc cttgacgatc acgccctccc gggcgctctg ttccatcaaa ctgtttcctc
      541 ctattccatg gtcacggact gctccagata gagcttcccg tccacataga cccggcagga
      601 cgcgctgccg ccgtaggcgg agctgagggg cgtgatcacg ctgccgctct gcccggcggg
      661 gtatacggcg gaatactgct ccacctcctg cccgttctcc atcagcgtta cccgaatggt
      721 tcgatcctga tcggcctttt ccatggtaaa gctgatctcg cccttatagc ccttgctctg
      781 cgtcgcgacg gtcagtacca ccggcgcgtc caacaccgcc agcgtccccg aaacgggatt
      841 ctgggccatg accatgccga tctggctttc gtccagcacc tccacataga cggtctcgcc
      901 gacggaaagc tggttttccc gcagcagctt ggccgcttct tcctcggtgc gggcggtcac
      961 gtccggcacc agcgtactgc cgccggaaat ggacagctcc agctcctgcc cgggggtaaa
     1021 ggcttcgccg cccttgggat tctgctgcag aatggtgccc agcggctctg tggacactga
     1081 tcggatgacc acgatgctga agccctgctt gcgcagattc ttggaaatat tgtcatacag
     1141 ctgccccacg caatcgggga tggtatcctg actggggccc agactgacgg tggccagcag
     1201 actgtcgccc cggctcattt ccgtgtccgg ctccggcagc tgggagatca ccaccccggc
     1261 ggggatgagg tcgctgttct gctcggtctg ctgccagccc agcccggcgc gtttggccat
     1321 cgccatggcc tcctccaccg tgtagccctc cccgtccggc gcaatgaccc ggttggtcac
     1381 ccggttgaaa atggccagaa tgccgaaggt cagcacggcc agcaccgcca cggtggccgc
     1441 ggcggtcaaa agaatgcccc ggcgagaata acggtgcttc ttcatccgcc ccgtgacctg
     1501 tgaaaccggg ccgggccggt agggtttgtt ctcggcaggg cgtttcgggg cctgcaccgg
     1561 ctgctgcctg ccnnnnnnnn nngcaggcag ccgggaaaag cccgtctgca gcgtttcggg
     1621 atccagcttg ccgtccttgg cctgcagcag cgcatccgcc atttcccggg cgctctggaa
     1681 gcggtccttg gggttcttgg ccagtgcctt catcaccacc gccaccacgg cgggcggcgt
     1741 ttccggcgcg tagtcggtaa taggcggcgg cgcgtcattg atgtgctgca tggccacgga
     1801 aacgggggta tcgcccacga agggcacccg gccggtgagc atctcgtaca tgaccacgcc
     1861 ggtggaataa atatcgctgg tggcctccac cacgccgccc atggcctgct ccggcgagga
     1921 gtaatgcacc gaccccacca ccgtatcccc cttggagatg gtgaaggcgt tggtcatgcg
     1981 ggcaatgccg aagtccgcca ccttcacgtg gccgtcggaa tggatcagca cgttctgggg
     2041 tttgatgtcc cgatggatga tgccgttgtc gtgggcgtgc tgcagggcgc ttaaaatgcg
     2101 cacggtgatc tggatggcgg tggtcgtgtt cagccgtccc ttctggcgga tcacgtcctt
     2161 cagggtgctt ccgctgacgt attccagcac cagatagcgg tattcgccct ccacgcccac
     2221 gtccagcagg ttgacgatgt tgtgatggct catcaggctg gcggcctgag cctcccgctg
     2281 gaagcgttcc aaaaattcct tgtcggaatt gtattcgctt ttcaggattt tgaccgccac
     2341 gctgtggccg gtgcggatat ccaccgcccg gtagaccagc gacatgccgc cctgaccgat
     2401 aaagtcctcc agctggtagc gctcggcgaa gatcatgcct ttcatcattc gccctgcacc
     2461 tccatcaccc ggcccagcac gaaggtgata ttgtccgtgc cgccgtgctc cagcgccagc
     2521 tccagcagac ggtctgcggc cttttcctca ttcagggtat tcaggatctc ttcgatgtcc
     2581 ttgtcgtcca ccatgttata caggccgtcg gagcagacca gccacaggtc gtccagctgc
     2641 ttgtcctcgg acagcacatc cacctccacc accgggtcga tgcctacggc ccgggtgatg
     2701 atgttgcggt agggatgctt cttcgccatt tcgggggtga tgacctgatt gcgcagaagc
     2761 tccccgacca cgctgtgatc ctgcgtgatc tgactgagct tgccgtcccg caggcggtag
     2821 gcccggctgn nnnnnnnnnc tcgccctccc acagaagggt catggtggtg cccatgccgg
     2881 tcaggttttc gtcgctgccc gccatgccgt atacgcggcg gttggcggcc tccaggccca
     2941 tgcgcagcgc gtcctcctcc ggtttttttc gggagagggc gtttttgacc acctgcacgg
     3001 ccacccggct ggcggtttcc ccgcccttgt ggccgcccat gccgtcggcg acgccgtaca
     3061 gccgtccctc cgccagcagg ctgtcctgat tgctggcccg taccagaccc tgatgtgttc
     3121 tgtggcaaac ccgcatgatg gtgttactcc tttgatgttc cttccagttc ccgccgccgc
     3181 agctggccgc aggccccgcc gatgtcggag cccatttccc ggcgcacggt ggcggagatg
     3241 tgacggtcct ccagcgtgtg caaaaagcgc tgcaccgcct ccggcgacgc ggggcgcagg
     3301 gcccgctcgt ccacatggtt caaggggatc agattcacat ggcactgcag atccctaagc
     3361 agcgccgcca gttcgacggc atgctccggg cggctgttga gccggtcgat gagcgcatac
     3421 tcaaaaacca cccggcggcc cgttttcttc acatattccc gcacggcggc catgacctcg
     3481 ccaaggggcc agcgccgggc gacgggcatg atctgccgcc gaatttcgtc attgggggcg
     3541 tgcagggaca ggctcagcgt aatgcccaga ttttcctctg ccaggcggcg aatctgatcc
     3601 gccaggccgc aggtgctgac cgacacgttg cgcaggctta aattcagccc tttttcctcg
     3661 ttgaccgccc gcagaaaccg cacggtgtgg tcgtaattat ccagcggttc gccgctgccc
     3721 atgagaacga tgtgccccac ccggcccttc tcgcccagat agcggttggc cagcagcacc
     3781 tccgagagca tttccccgct ggaaaggctc cggacgcagc cgttcagggt gctggcgcag
     3841 aaggcgcagc ccattttgca gcccacctgc gtggagatgc acagggagta gccgtaatgg
     3901 tagtgcatca gcacgccctc gatcaggttg tcgtcccggc agcggaacag aaacttgacc
     3961 gtatcgtcct tttgggaggg aaacacgccg tacagctcca tgggcagatc gtcgcaggtt
     4021 tccatcagct gctcccgcag ggttttaggc agattgctca tttccgaaaa gggcacgccc
     4081 cgggtgagcc agctgtacac ctgaccagcc cggaaagcgg gctggcccag ctccttgaag
     4141 taggctccca gctcgccggg ggtcatatcc agcaggatgc gtttttccgg catcagtagt
     4201 tcctccgcag acggacgata tagaagccct ccacctgatc tcggaagccc agcagctgca
     4261 gacccagcgc gtcctgctgc tggcgcagct ccgccgggaa ggacaggggc atgggttcgg
     4321 tggtatattc aggatgctct ttcaggaagg cttcgatctg cgccttgttt tcctccggca
     4381 gcacagagca ggtggagtac accagcagac cgccggggcg aacgtagcgg cacaccgtgt
     4441 tcagcagctt tttctgggtc tccacgatgg cgggaaggtc gctttccttc aggcgcagct
     4501 tgatatccgg cttctggctc aatacgccgg tgccggagca gggcgcgtcc agcagcactg
     4561 tgtccagcgt tttttccata tcgagccggt actcgcaggc gtccttcacg gcggtgcgca
     4621 ggttgtccag ccccaaacgg cggcgaacac catccagcag gagcgcccgc ttttcgtgca
     4681 gctcccacgc atagacccgg cccgtcagct gcatggtctc gcacagataa gcggatttgc
     4741 cgccgggagc cgcgcaggcg tccagaattt tcatgccggg ccgggcctgc accgcttcgg
     4801 cggccagcat gctgctctga ccctgaatgg aataaaggcc cgcctgataa tccgggtcaa
     4861 agcccggctc ggtcacgccc tccagcagat aggcatgggg agccacgcct gcccggaagg
     4921 acagattttt tttacggagc aggtcttcaa aaccatggtc gtccagcttg agcagattgg
     4981 gccggacggt ggtgggatgg ctggcttccg ggtctttttc cgcatccggc caggacagct
     5041 cgtccttttc ccggatcagg ttgcgcagca ccgcgttcac aaagcctgcg gaggcttcca
     5101 tgcccatcgt cttgaccagc ttcacggatt cgtccacggc ggcgctgtcc ggaacccgct
     5161 ccaaaaacat gatctggtaa acgcccagcc gcagcgcgtc ccgagtgacc gcgtcccggg
     5221 cgggatactc ggtcagctgg ttcagagcat ggtcgatctt cacttcgttt tccagcgtgc
     5281 cgtacaccag ttgcgtggtc aggcggcgct cctcgggctt caaatcacga ggcagggtct
     5341 tattcaggct caaggcagca taggcaccgt tttcgtgtac atccagcaac actttcagcg
     5401 ccagcttgcg ggcgctgggc tggacgggcg cggcaggctt ccggggccgg ttgccgtaag
     5461 ccggtttgcc ggagggtctg ccgaaggaag gctttccgga ggcgggcttt tcaaaggagc
     5521 gattgccgga gggctgggga cggcgttctc cgtcaaactt ccgggggccg gaggcagtct
     5581 tgccgtcata ggggcgcttc tcgtccttcc gggcgttttc tccctgcgga cgggcagggc
     5641 gatggccttc gggcttcccg gcatagggac ggcgctggcc gtcaaacttg ccgcccggac
     5701 ggcggtcgct tcgtttttcc ccggcgttgg acgcggggcg ttcgttctca ttcatagctt
     5761 tgcctcactc aatttctgtc cttcgggaat ggtgtgaccc agcaggtaag ctttagcttc
     5821 catccgcttt gcgccgggag cctgaatttc gcacagttca acggctccat ccccagtggc
     5881 cacgatcagg ccctttttcc ggtctgcgca cagcaccgtg ccgggctggc cgcagccctg
     5941 cgcggcgcgg gccttccaga tcttcagcgc gccattctcg gtggtcacat agccgcaggg
     6001 ccacgggctc atggcccgca cccggttcac gcactgggcc gtggtctcgg taaagtccat
     6061 ttcgcccatt tccttggtga tcttggggtc gtaggtcatg cggctctcgt cctgcttctg
     6121 ccgggggcag tcgccggcct cgatgcggcg cagagtttcg atcagcagtt cgccccccag
     6181 atgggacagc cgctcggtca gttccccggc agtctcctcc gggccgatgg gcgtttcccg
     6241 ctgcagcaga atatcgccgg tgtcgatgcc cgcatcggtg aacatggtgg tcacgccggt
     6301 gacggtctcg ccctgccaga ccgtccactg gacgggcgcc gctccccgat gccggggcag
     6361 cagggaagta tgcacgttca ccgtgccgat cttcggcaca tccagcactt cctgagaaag
     6421 gatctgaccg aaggcggcgg tgacgcacag gtcaggcgca aggcttttca ggtcgtccag
     6481 accgtccttg cgaatcttca agggctgaaa tacgggaatg ttctgcgaaa gggcgtattc
     6541 cttcaccggg gtcatctgca ccttgccgcc ccggcccttg ggtttatccg gctgggtaaa
     6601 gaccccggcc acctcgtagc cgttttctac cagcgcgcgc aaaataggaa cggaaaactc
     6661 cggcgttccc ataaaaacaa ctttcattcc ccgtcctcct cttcgtctcc gtccaccggc
     6721 tcgtattcct cgtcgccctc caaaatctcg cgatccatct tgtccacata gagaacgccg
     6781 tccagatgat ccagctcatg acacacggcc cgggcaaaaa actcctcggc ttccagctcg
     6841 atggcgttgc cctccgggtc ctgagcgcgc accttcacgt gattgggccg ggtgactacg
     6901 ccgctgcggc cggggatgct caggcagccc tcggggccgc tctgctggcc gtccgactcc
     6961 acgatgaccg ggttcaccaa ttccaccagt ccgtccccca cgtcgatgac cacggcccgg
     7021 cgcaggatgc ccacctgcgg cgcggccaga cccacgccgt tggcgtcgta catggtgtcc
     7081 gccatatctt ccagcagaat tttcagacgc cggtcgaatt tttccaccgg cttgcaaatt
     7141 ttgcgcaact tgttgtcccc gatcttcaca attttcctca gcgccatcga aaatctctcc
     7201 nnnnnnnnnn gcttgagcag cacatgctag cggtactgcc cccggatgcg cttgaccggt
     7261 gcttcgtccg cccggatcat cagcgtcttt ttcttctgca ctgggtggct cagaagatag
     7321 gtctgcaggg tatcgtacag ccgctgggac gtatcttttg cctcggcctc gtcggggctt
     7381 tccaccagca gacggcacag catggtaaag ggcggataca gtcccgcccg ccgccgggaa
     7441 aactccattt cgaaaaaggc ccggtagtcc tgcttcgctg ccgcctgaat gcagggatcg
     7501 tccggcttgt agctctgcac gatcacctcg ccgggctcct gcgcccggcc cggcccgcca
     7561 cctgcgtaat gagctggaac gtccgctccg ccgagcggta atccggcagg ttcagcgtca
     7621 tatccgccgc caccacgccc accagcgtca ccttgggaaa atccagcccc ttggcgatca
     7681 tctgcgtgcc gatgagcacc tgcgcccggc ccttgccgaa ctcgtccagc aggcgggcgt
     7741 gggcgtcccg gccccgggtg gtatcaatgt ccatgcggac ggttttgacc tgcggaaaga
     7801 gcttgtgcat ctcctcctcc acccgttggg tgccgctgcc gaagtagcgg atgtacttgc
     7861 tgccgcactc cgggcacacc gtgggcggcg gcttgaccgc gccgcacagg tggcagcgca
     7921 gctttcccgg atcctccggg ctttcccggt gcagggtcat actcaggtcg cactgctcgc
     7981 acttgaccac atagccgcag tttcggcagc tgacgaaggt attgtagccc cgtcggttca
     8041 ggaacaacat ggcctgatgg ccgctgtcca gacattcctt caatttctgg atgagcagcg
     8101 cgctgaaaat ggagcgattg cccgcctcca gctccttgcg catatccacc accgtcacct
     8161 gcggcagcgg tcggtcgccc acccggcggg gcatttccac cagcgtgtag tccccccggc
     8221 gggccatggc aaaggaaaga atgctgggcg tagcgctgga caggatcagc gcggccccct
     8281 cgttttcgca gcgctgcttc gctacccggc gcgcgtcgta gcgggggtgc ttatcgctca
     8341 gataggtctg ttcgtgttcc tcgtcgatca cgatcaaccc cagtttatcc accggggcga
     8401 agatagcgga acgcgcgccc accaccaccc gggcctgtcc cgaacggatg cgccgccact
     8461 catcatatcg ttggccgtcg gtcaggcggc tgtgaagcac ggcggtgttt tcgccgaagc
     8521 ggctgtggaa ccaacgcacc atctgggggg tcagggcgat ctccggcacc agaatgatcg
     8581 cgcccttgcc tatttccagc gtccgctgca ccatggccag atagacctct gtctggccgc
     8641 tgccggtcac gccatggagc aggaacgcgc cctgtccctt ttccagcgag gggatcatgc
     8701 tgtccagtgc ctcccgctgg tcgtcggtca ggggcggtgc gacggtacgg gccggttcca
     8761 gctccgtatc cggggcccgg aagacctcct tctcggtcag cgtcaccagt ccccgctccc
     8821 ccagcgcttt cagcgcgtcc cggggattgg acaccagctg ggccagctgg ccgaccccgt
     8881 gaggctggcc gtccttcaaa agccgcagca ccatggcccg cttgggcgcc cgccgcagcg
     8941 cgtccgcctc cttctccgcg tccacgccgg ggcagagcac ggcgcagagc tctttcttct
     9001 gctggatgcg cccggtgcgc atggcggcgg gcagcatcag ccgcagagtt tcggacaggg
     9061 ggcagtgatt ttcctccgcc agctgacggg ccagcgtcag aagctggggc agcagggcgg
     9121 gataatcgtc cagcaccgcc gctatgggcc gcagtttttg cagcggcacg tcggcgggca
     9181 gctggtcttc ctccaaaagg gacaccacaa agccgtccac ccggcgcttg cccagcggca
     9241 cgctgacccg gctgccctgc tccactgcca tgccctcggg tatccgatag gaaaaggggc
     9301 ggtctacgtt ggtatgcacc acgtccacga tgacctgtgc gatcatgtcc gctccccctc
     9361 tcctgtgttt ttacaagttt cttaacggaa gtaccgattt cgaggccggg gaacccggct
     9421 ctttttatgc cgcccggtct tgtgcagtgc ccggcggatg aggtgaatga tgaggaaaat
     9481 gacggcgaag ggcgtcaaca ccaccagcgc cagctccacg gacaggggcg gcagcggatt
     9541 gggatcagcc ttcacctcgg cctcgatctc ctccagcgtc aggggggcgt tatcccgccg
     9601 ctggatggtg cgcccggcga tcagatcgta gaccacgggg ctgccgccgt cggtgggata
     9661 ataggtcatg gtgcccatga cctcgccctt ttcaatgggc gcggagaaat cgcgcgtata
     9721 ctcgatcagc acggtctggc tcaggttgcg ggccatggcc tccagatcgc ttttggtgcc
     9781 gatgatattc accgtgcggg tggaggcctg cgcccgcact tccagcggca gacggcccaa
     9841 gtccgcatcg tccagcgaga agccggaggt ctccacggtg atgggatttt cgttgtacag
     9901 ttccagcggg gtcatgctga cgaactggct gaagccgtat tccatcagct ttttactgtc
     9961 cgaccaacgg ccggatttgc tggtgtagaa cagtacggag atcagctcca ccccgtcccg
    10021 ctccgccgcg cccacgtagc aatagcctgc ggcggcggtg tagcctgtct tgataccgat
    10081 ggcgtcagga taataagtcg aattggcccc ctccgggtcg tccttggtgg cctgattggg
    10141 gttgatccag ttctcgtttg tgccggtgat cgtccgttcc ttacgcacat tgctgctggg
    10201 cagggtatag gagaaggtct tggcgatctg gcggaaggtg tcgttctgca tggcggcctg
    10261 agcgatcttt gccatatccc gggccgtggt gtaatgattg gggtcgtgca gaccgctggc
    10321 gttggcgaag tgggtgccag tgcagccgta catggcggcg gcttcgttca tcaggttggc
    10381 gaattccgct tcattgccgg agatcagctc ggcgatcaac tgcgcgccgt cattgccgga
    10441 acggatcatg gtggcgtaga gcagatccag aaaattgatg gtctccccca cttgcagacc
    10501 ggtagtggag ctaccctcct ccacctgcgc cgcactttca gtcatggtga cctcctgtga
    10561 aaggtcgccc atcatcacgc ccagcagggt cgtcagaatt ttggtggtcg aggcggggta
    10621 catggtctgg tcggcgttct tttcaaagat cacgtcgccg gtggtggcct caatgaggat
    10681 ggccgccttg gcgtaaagct ggtcggcgct cagctcctcc ggcttttcag gatcgtaggg
    10741 gtccgcgctt ttagacaggg taggcgctat gtaggtatat ccgtttccat cctccgcccg
    10801 gaccgcggga acaggcagca gcatcagtac cagacagaac gccgcaaaca aagcggcaag
    10861 ccgttttctt ctttctttca tccgcctttc cctcctctcc gtaaagggga taccagtgcg
    10921 cctcacatcg gccatgcagt ccgaaggccg cagcatccgc cagagcctat gacaacactt
    10981 tattatacac ggaataggaa atttgtacaa gcaccggccc atccgggggc ggaagccgcc
    11041 cgaaaagcga aattgtacca tcggtttaga attttctgtc ttttctgtac caatttcatc
    11101 ttttcccacg gtgtttcccg tggtacatta aaaacaggcg ctgcccaagg cggcgcagga
    11161 aacggaggat ttccttatga agctatccct tgcccaaaat atctgccgtc tgcggaagca
    11221 aaacggcctg acacaggagc ggctggcgga agcgctgggc gtgaccttcg ccgccgtgtc
    11281 caaatgggag cggggcgtgg ccacgccgga cttaggcctg atggcggagc tggccagcct
    11341 gttttccgtg tcgctggacg cgctggcggg cttcgagctg cagcaaagct ccgcagaggc
    11401 gctggcccgg cggctcctcc atttgcagcg ggagaaacgc ttcggggaag ccgtgtccga
    11461 ggcggaggag gccctgcgcc gctatcccaa cgatttccgc gtcgtgtacc gctgcggctc
    11521 cctgtacgag ctggcggggc tggagcagaa ccggccggac tggcttcgcc ggggccggga
    11581 tctgctggag cgctcccttc ccctgctatc ccagaatacc gaccccgccg tcagcgatat
    11641 tttcattcag taccatatcg cccagtgctg cctcaagctc ggcgaggggg atcaggcgct
    11701 ggagattctg aaaaagacca acatctgcgg cgttcacaac gccctgatcg ccctgatttt
    11761 gagcagtggc cgtcatcccc tccgggaagc ggagtcctat ctgaccgacg cgatgggcga
    11821 tctgaccagt ctgtcggtac ggctgatgac ggcctatgtc tattactatc aacgaaaggg
    11881 cgatgactcg tccgctctgg acgcgctgca gtggctgtgc cacctgctgg attccctaaa
    11941 gacgggcccg gatgccgtga gctatgtgga taaaattctg gcctgctgtt gcgccgagtg
    12001 cgcccatctt tcccggaagc tggggcggcc agaggaggca gaggactggc tacggcgggc
    12061 ctgccggata gggacggcct ttgacgcggc ccccacctac cggatggaca gcgtcaaatt
    12121 ttgtgcggga accgtctccg aggccaccgt ctacgacgat ctgggcgaat cggcgctggc
    12181 ctccgtggag cggcagctga cggaggaaga ccatgacgac gannnnnnnn nngaaggagg
    12241 atcagccatg aaaaaccctg cctctatggc tcagcctgac ctgcggccct acacccggac
    12301 gtttttccgg ggcaaccgac taaattttgc tctggccctg acggacgttc tcctgagcgc
    12361 cgcggccaat ctgctgatct cctggcttct gcagcagatt atggacttga tcggcggcag
    12421 cggcgcgggc tggacgctcc gtcagctggc ggtcatcgcg gcggggctgg tggccggggc
    12481 ggctgtctcc tgtctgtttt cctacttttt caagccccgc tttctgacta gggccatggc
    12541 ccagtaccgg gaaactctgt ttggacgtct gatggaaaag ccgctggccg ctttcaccgg
    12601 ggaaaactct tccctgtacc tttcggcgtt gaccaacgac ctttctgtga tcgaaaaggg
    12661 ctgtctgacg aatcttttct ccctgacgaa tagcctgctg cttttcgtgg gtgcctttgc
    12721 catgatgctg tggtacagtc ccctgctgac gctagccgcc gctttgctgg cgctgctgcc
    12781 ggtgaccgcc tcccttctga tgggcgggaa ggccgccggg gccgaaaagc ggctgtccga
    12841 tcagaacgaa gcctgcactg ctgcgctgaa ggacgcgctg ggcggcttct ccgtcatcaa
    12901 gtcctttcag gcggaaaagc agatgtgccg actgtttgca gacagcgtac gcagcctgac
    12961 cagcgcccgc tgccgcaagg aacgcatcgg cattctcatt cagaggatgg gcagtctggc
    13021 gggcgtaacg acccagttgg gcgtgtttct gataggcgcg gcgctggccc tgtcggggaa
    13081 ggggctgacg gtcggtacgg tac
//
LOCUS       DAWAEA010000006        11846 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_863_length_11846_cov_2.545670, whole genome shotgun sequence.
ACCESSION   DAWAEA010000006 DAWAEA010000000
VERSION     DAWAEA010000006.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 11846)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11846)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11846
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_863_length_11846_cov_2.545670"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1315..1324
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4153..4162
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10199..10208
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10479..10488
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2183 a         3661 c         3585 g         2377 t
ORIGIN      
        1 ttcacataag aggtcttgat ggtcagttcc ttcttgaagg cctggaacgg gtaataggga
       61 atggcgcagt ccgggtcggt caggccgaaa atcatggccg tggcggcctt gcccgcgtat
      121 tcgatggcgt actgcaccgt ttcgggccgg cctacgcatt cgatggtgac gcggatgtcg
      181 tcaaagccgt ttcgggcaag ctccgccttc acgtcgtcct tcaagggatt cagcaccaga
      241 tcggccccca gcttccgggc cagatcgaag cgcttttcgt taggctccag catgacgacc
      301 cgcaccgcgc cgcaaagccg cgccagctgc agcatcatca tgccgatggt gccgccgccg
      361 atgaccatca ccgtgtcgcc gggcttgatg ccgcacagat caatgccgtg cagggcgcag
      421 gccaccggct cgcacagggc cgcttcttca aaaggcacgc tgtccggcac cttgaacacc
      481 tgccgttcca gcacggcgca gtattcggca aagccgccgt cgtagttgac gccggtggcg
      541 gccatggagt cgcagaagtg gggattgccc cggcgacact ggtcgcaggc gtcgcaggaa
      601 atgttggggt tcacagtcac atggtcgcct accttcaccc gggtgacggc gctgcccacc
      661 tgcgtgacga cgccgctgaa ctcatgcccc agaatcaccg gcgggttgca ttccgtcgcg
      721 ccctgcgcgc cgccgtaaat gtgcacgtcc gggccgcata cgccgcaggc cttgacctga
      781 atcagcaccg actccggccc tacctccggc atgggataat gctcgtctac tcgaatgtcg
      841 ccctttccgt aaaataccgc tgctttcata aaaatggatt cctcctttag taagtatatg
      901 gtttagggat gcgcccatag cgctttttcc cttccatcct tattcttcca gcttgggtcg
      961 cccatgttcc agaatgcggc gggccatttc cttgctgatg ccctccacct gacaaagctc
     1021 gtcctctgtg gcgttgaaga tggcctttac actcttgaag tgacggatca gctccgtctg
     1081 gcgcttggga ccgatgccct cgatggcgct tagctcgctc tggatgctgt ttttgccccg
     1141 cagggcccgg tgatgggtga tgccgaagcg gtgagcctcg tcgcggacgt actgcaccag
     1201 atgcagggcg ttggagcgcc ggtccagcac gatggggtcg ggcttgccgg gcaggtaaat
     1261 ttcctcaaag cgcttggcca ggccgaacat gggcacactg gctccggctt cctcnnnnnn
     1321 nnnngccctg ctcctcccgc tcgtgctttt cctgcaggcc gtgggtaaag cgacggccga
     1381 tgacctccgc catggaggcg aagtcgttgg ccccctccac ggtcttgatg cggaagtgac
     1441 ggtattcctt cttggcgggc aggccgccct cgaacaccac catgctggcc acgctcagga
     1501 tgccctgcgt gttggaaata tcgtatccct cgatgcgccg gggcaggttg ggcaggtgaa
     1561 gggcctccat cagctccttc atggccaagg tggtacgcat gtacttgacc tgctcgcttt
     1621 ggtaatgatg atccagcgcg ttctgggcgt tcttttgggc cagcttcacc agctccagct
     1681 tgtcgccccg ctggggcacg gtcagggtga ccgccccgcc cttgcgcagc cggagccact
     1741 gctccagctc ctccaccggc tctggaaggg tctgcaccag cacgttgcgg gggatcagat
     1801 gctcgtcgtc gtaatactgg gtcaggaatt ggctcaagat ctgggccggt tcctcgccgc
     1861 cgcagccctc catcaggatg gagcggcctt cctccatgcg gccgccccgg atgtgggtca
     1921 gctgcaccat ggcgtcctgc tcgttcatgg ccacggccag cacgtcctgc tccacgtcct
     1981 tggtctggat ggcgtgctgg cgctccatca atccctggat gctgtagagc ttgtcccgca
     2041 gctcggcggc gcgctcgtac tccatccgct ggctggcggc gatcatttcc ttttgcagct
     2101 catacaccag ttcccggctg tcgccgttga ggaaggccac cacccgcttt aggatggcgg
     2161 cgtagtcttc ctcggagatc aggcccgcgc agggggcctt gcattcgcct acctgatggt
     2221 gcatacaggg ccgccggggg gctttcaggg gcagggtcag gttgcaggta cgcaggggaa
     2281 agaaccgccg cacctcatcc atcacctgcc gcaccgtcgt ggccccgatg taggggccga
     2341 agtacttggc gccgtcccgg ctgtccatcc gccgggccag cgtcagccgg ggatagtgct
     2401 cgtgaaagtc gatgcgcagg tagggatagt gtttgtcgtc cttcagaaga atattgtaga
     2461 agggcttgta tttcttgatg aggttgcatt ccagcgtcag ggcttccaga ttggtctggc
     2521 acagcatcag gtcgaaatcg tccacccggc tgaccatggc cgccaccttg ggggtgtggc
     2581 tgccgtggaa atactgccgc acccggtttt tcaggttgac ggccttgcct acgtagatga
     2641 tctccccgcc gcttttcatg atgtagcagc ccgggctttc cggcaggagc cggatttttt
     2701 cctgcaaaac gtcattcatg ggaaagctct tccttctttc cgcgggtata gcgccgccaa
     2761 acgggccatg cgaaggcaag gcccatcacc agatagacga tcatcaccgc cacccgctgc
     2821 atcagcgagg cggccacgcc gctctggaac agcgctccca tctggctggc ggccacgccc
     2881 agcacggcct ccttcaggcc gatgttgccc ggcacgatgc tcagcaccgt gctcagccag
     2941 ctcagactgt tatacaacag cacctgatgg aacggcacgg gcgtgcccac cgcccggaag
     3001 cactccatgt acagcagaat ttggaatacg ttgttcacga tgaggcaaag aagcagctga
     3061 cgcagcatct tccggctgcg cagaagggcg ttgaagccgg tgatcacatc ccggagcagc
     3121 ttgtgcttct gcacaaaggc gggctggtga tcctccagac gcagggccag ccacaggaac
     3181 accgccacgc acaggatgcc cgcgcccagc gccgcccaca gaagaagcgg ccactggccg
     3241 ttcaggaagc ctgcgcacag aagcgccgcc agcatttgca gcagggcgaa catcacgccg
     3301 aacaccacgt tgcccgccgc catgctgacg cttttcacat acgccagccc cttggcccgc
     3361 ttgtaatacg tggcggaaaa gatcaggtcg gcccgcatgg gcagcacgta ggcgatggcg
     3421 ttggccacga acaccacgcc catccagtcc accagatcca gcggcacatc gtatacgctc
     3481 agaataatgc ggtggtagac gcagttcatc acgcagccgc acagggcgaa caggatcagc
     3541 agcgtcaccg tgcgcccgtc cagcgtgagc agctcccgca tttcgtccca gtggcggtac
     3601 aggtaccagc ccagcaggcc caccagcacg ataaacagcg ccatggagag aatctttttc
     3661 cagttcttgg gcagcttcat tgcttcgctt ctttctttca gcaggcttta ttttgttttt
     3721 cctgctcttt ttccatggcc cgcttggcca cgtcgttggt gtcggtcagg ttgcggtaga
     3781 ccacgaactt ctggtacaga aattccagaa cgaaattgag gagcatggtg agcaccagca
     3841 ccaggtcttc gttccagccc ccgttggtca gggcgctgcc gccccaggtg gacagagggg
     3901 tgaacaccgc gtagaacagc gccaccagtc ccatggcccg gggcacgctg gcgtcgctgc
     3961 ggaaggtgta gcggcggttg aaggtgaagt tccataaaac gctcaggcac agcgccgtca
     4021 ggtagctgcc ccagtaaggc agcttcagcg ccttggacag aagctcgaag ctgaccacct
     4081 gaatgaggcc cgcgctggcg gagaaaaggg taaacttgat ggcctgccat gcttcggctt
     4141 tggccttgcg gtnnnnnnnn nncaaaagga cttccccagt ataacacaga tagcgccgaa
     4201 aggcgaacac ttgcatcctt tcccgcgcag ggatataata acaatggaga ttttcaagat
     4261 tgcaagaaac cgcgccgccc tttcgttttt tggggggaag gcggcggaag aaaggctacc
     4321 gtcatgcaga gagtgaaacc gagaatgagc cgtcgcaaag cggtgctgat cctgatcgcc
     4381 gcgctggccg cggcgggcct tctgacgtgg ggaattctgt ccctggtgca gggccagcag
     4441 accaccggcg ttaccgctac caggctgccc tgtccctacg atgaaaacat caaggccttc
     4501 ggcaataacg tgctgtacta cgacggcatg agcatccact gcatgagcgc cacgggcgcg
     4561 gtgcgctggt cgttccagct gggaaccaac gcgggcttcg actgcgacgg cgagcacatc
     4621 gccgcgtggg tgggctccac cgtgtatatt ctggaccaga acggcaattc cagctacaac
     4681 gacaatctgg gcgaggaagt gcagttctgc cgcatcggca agcagtatct gggcgcggtg
     4741 gtcggcgaga ccgattcccc ccgtctgctg gtaaaggaca tggcgggcgc gcacatggac
     4801 gaggaagccg acgcttacaa aaacctgatc ctgctggact tgggcttcta cggccccaac
     4861 ggcgagtata tgtggacgct ggcgctggac gtgttcggca ccgccgccaa cagcatcctc
     4921 aacaccttcg aggtgggcaa gatgaatacc ggcgaggtgt cgctgggcga gaatatcacc
     4981 taccgggtgc tctacgacaa cggcgtgctg cgggtcatca acacccgcaa gatgcgtgcc
     5041 tttaactatc ggggcaccga ggacaccgcc cagagcgtgc tggtatacgg ctggcggctt
     5101 ctggacgatg aaattcccga acgcggcaac gcgctgatgc ttttcagccc cacttcccag
     5161 tccgacagca tttacgatat ccgggagctg cggctcattt ccggctccac ggtggataag
     5221 cgctacagcc tgcccgccag ctgcatcggc gcgacggtct ggaaccgcac catctacgcc
     5281 ctgtccgcca ctcagctttt gcgggccgga ctgaacgaca gccgttttac caccttcgac
     5341 ctgccccttg acagtcaggc cactcagctg gtggacaccc tcagcgacgg acgcatcatc
     5401 gtggcctgcg gcgaagaagt ctacgtgctc accctgcccc ggggcaatct cgtcagcggc
     5461 gtatgaccgc cccttttcaa aggaggaatg aagcctgaat cccattgatt tcgcggccct
     5521 tgccatcatt ttgatctgcg tgcttttcgg cctgtaccgg ggttttatcc agtcggtgct
     5581 gagccttggc ggaagccttt tgtccatcgt gggttccttt ctgctttatc cccggctggc
     5641 ggacgtggtc gcgtccaata ccgacatcac ccgcatgatc tccacctata ccgactccca
     5701 gagcctgttg ggcgatctgg atttgagcag tcaggcggtc agcgggctgg ccggggacgc
     5761 tattacgcag gtggtgcaga aggccaattt gcccgagcct atcggcaccc ttctgcagca
     5821 caacttgtcg gcgcaggtat tcagccccat gggcaatctg gccaccaacg tgggcgacta
     5881 cgtgaaccag accattttga gcgtgtccat caacgtgttg tgcttccttt tgtgcttcct
     5941 tgtctgcttt atcgtggtgc atatcctcat caatctggtg cgggccgtgt tccgcttccc
     6001 ggtgctcaag catctggact ggctggcggg cggcgcgttc ggctttctgg tgggcgtgct
     6061 gctgtgctac gtctgcttca ccgtgctgcc cctgatggag agcgtcatcc ccctcgaaaa
     6121 cttccgcacg ctggccgatc aaagcgtgct ggccaagacc ttctccgaca gcagcctgat
     6181 cgtctccatc atgaaccgaa aactgtaaag ctcttttgaa aaagtgaggg gtctcccctt
     6241 tctttttctg ttttctttct tttattttca ttgtcccgga ggtacgccat gagcgaattt
     6301 ttggcccccc tgaactacga ccgcgcccgg gaagccctgt gccgtctgag cgcctacggc
     6361 ggctacgacg agcagaaggc cgaagaagcc atgggggtgc tgcgggaatg ttacccgctg
     6421 gttttttcca aaaccgaaca ggagctcttt cccaagggaa gcgttctgct gcacctgcag
     6481 ggagccagcg tcaccgaccc gctgatcttc accggccatc tggacgtgcc agacgggcag
     6541 gccacgccgt tcatgcccaa tgtgccggac attcctctca gtgtgcccat gagccgcgcc
     6601 catgtagtgg ctttattgga agcgctggag gagctgctgc gggacggcta ccgtccgggg
     6661 ggcgacctgt ttctggccct gagcatggac ggtctcagcg gggcggaggg agcccgggcc
     6721 atggcggact atttgcaaaa gctgaacatc aagccctgct ttgtgctgga ctatggcggc
     6781 tacgccacga tggagtcctt ccgaagctat ctgcccgcga acgtgccgct ggccctgatc
     6841 ggcgtggcgg aaaaaggcct gttgatgggg caggtggaag cggagcaggt cgcgccgggg
     6901 gacgaccctg cagcgcggct cttacagggc ggcgcgcgtc tgacccgacg gccccggcag
     6961 gcgtctctct gcacggccag ccggaagatg ctggaagcca tcggccgcaa aacgcccggc
     7021 tttttcaagc ggacgatcat gctgcatcct ggcctgtttt tcccgctgtt gcgctgccgt
     7081 tggcacggcc gttccgtttt tcatcagttt ttcctctcgg agctgacggt ctaccatctg
     7141 gcggcctacg gcgaaccggc ctctccggcc cggcggggtg agctttgttt ccggcagacg
     7201 gtgatccccg gccagaagac ggcccgattc cgtcagcgcc tgcgccgccg cctgaaggag
     7261 cccggtctgc gcttccagac gcaggtggag tacgagcaca gccgtttgag cgcggccagc
     7321 ggcgaagcgt gggacgcgct ggagactgcc attgagattc aattcgagcg ggcggtgatc
     7381 gcgccctgtc tttgtccctt tatcacggac agccgtttct acgctgccgc gtgggacaag
     7441 gtctaccgtt tctccccctt cttagtcacc ggcgaggaag cgctatccgg cctctgcacc
     7501 gtcactggcc ccaaccttca aaccgccgtc caattcttcc gctccatgct ctccgtctag
     7561 gcaggggcag tgatccgcaa cctcaatcgt cgtctttggc actgccgtgc caaatcctcg
     7621 tttcggttga cggctgccgg tcgctaaccg cctgcggcgg ttgtgcccac tgggcgacgg
     7681 tgaacttttc aaataaaatt tgaaaagttc agtctgccaa aggcagaccg ggttgtccca
     7741 tggacaaccc gccttgcgtc ttcctctcgc cgcccttgac cccggactgc gcgccacagg
     7801 ggcatagagg gacatggggt gggatatgac cgcaaaggtt aaaacaaaca acgatttctc
     7861 aacgacttca gaaatgaggt tatcttatga agcaggatta catcaaggaa cttctatctc
     7921 aggcgaaggc cgccggcgtc gaggcggcgg aggcctatct gagcgaaagt gagagctttt
     7981 ccgccatgcg caacaacggc gcgctggagg actatcaatc caaccacacc cggggcttgg
     8041 gcttccgggg gctggtgaac ggccgcatgg gctatgcgtc caccgaagcg ctggacgacg
     8101 aatccatcgg tcagctgatc cggggcgtga tcgagtcggc cacgctatgc gaaagcaagg
     8161 acgagcagcc gctctatcag ggcggcgggc agttcccgga agtggcgctg tacaacccgg
     8221 agctggacaa ggtttctccc gaagccaagc tggcgaagat cgaagccatg gagaaccagt
     8281 gcaaggccac ggacgagcgg tatctgaacg gctacaatat gatcgacacc acaaagcaca
     8341 ccgtgcgcat cgccaacagc tacggcatgg atcagaccta cacggaaaac ttctgccagc
     8401 tgtactgcag cgccaacgtc aaggagaacg actgcgtttc caccggcggc tttgcgcaga
     8461 tcagccgtga ctttgacacg ctggatccca tcgcgctggg ccgcgacagc gtggagcagg
     8521 cggtgaaggg gctgaacgcg gagccggtgg cctccggcaa atatcgcgtg atcttctgga
     8581 atgaggcgct ggtcagcctg ctgggcgtat tcaacaccat tttctccgcc gagacggagc
     8641 agatgggcct gtccctcctg tccggcaagc tgggcgagac cattgccgcg ccctgcgtca
     8701 cgctggtgga cgatcccctg cgggccgact gcgtcggcag ccgtcccttt gacgatgagg
     8761 gcgtgccctc tcatcagcac atgctggtgg agaacggtgt tttccgcacc tttctgcaca
     8821 atctgaagac cgcgaagaag gcgggcgtgc agtccacggg caacggcagc aaggcggact
     8881 actcctcgcc ggtgcgggtg gctccctcca acctgtactt cgagccgggc gtgctttcct
     8941 ttgacgaact gctccggcag gtgggcgacg gcgttgtcat cacggaagta agcggcctcc
     9001 acgcgggcgc gaacccggtc agcggcgatt tctccctgct gtccaagggc tacaccatca
     9061 aggacggcca gcgggatcag ccgctggagc gcatcaccgt ggcgggcaac ttctacgagc
     9121 tgctcaaaaa catccgcgcc tttgccagcg acctccgttt cccctacggc cccatgggca
     9181 gcgccagcgc cgacgcgggc gaaatgacca tcagcggcaa ggcctgattt tcccttcgaa
     9241 aaatggaata acgtatacga tcggagccga aatggctccg atcgtttttc ccataacaaa
     9301 acgctccggc gcagggcttt tcttccctgc gccggggcgc attctatatt ccgaatttca
     9361 gcttagtccc cgatggcgtc cgtccgccaa ataaagcgca ccacgccttc cgcgtcgtcg
     9421 gccttgccgg cgtaggactg gtaggcttcg gtgtcatccg ccagagcgcg ggcgttctcg
     9481 aacaggggct tccattcatc gttgaccatg gaggtcagcg tttgcaggcc gtcctgatcc
     9541 agcgtctgca gccccgcgta cagctgggcc gcgccatcgt tcagagccgt agcaccgtcc
     9601 gtcagggcgg gcatgttggc gctgagctga tccacaccag cggtgtaggc ggtcaggccg
     9661 gtatagaact ggttgtagct gtccagctgg gccttgagca cctgcagact ctggaaagct
     9721 tccgtcttgg tctgcttggc ggcggtaatc tgctgggtca gaccgtccag cacttcggcg
     9781 taggtctcca ccgtcaggtc gggcacttgg ggattcaggg ccttgagctg ctggttcgca
     9841 gtttgcagca gcagctggaa gacctgcagc gcaccggcgt tcagggtctc gctgttttcg
     9901 ctcagggtgc tcagaccgcc ggccacctga ccaacgccgt cattcagagc ggaagcgccc
     9961 tcggacagtt gccgtccgcc atccaacagc tgggccatcg cgtccgtcag ctgatccata
    10021 tcaccatcgg cggcgtccgc caggctcagg tcaatgtcct tcaaaaggct gctccattcg
    10081 gtggtggcgg cggtgacggt caggggcagg gcgaagtctt ccacatcggc ggtgatctca
    10141 acggtctcgg gcagcttgcc gtccttgtct tccacgtcca gccgctcttt caggccggnn
    10201 nnnnnnnnat cgctgtccag aagggcggcg gacagaacgg caaaccgggc cttctgcccg
    10261 tcggcggggg tataggagaa acggatggtc acgcggccgc tcttaccggc catgtcggcg
    10321 gcggggactt cctcgccgtc caccagatag gtgatctgca gcgtcagggg cagctcctcg
    10381 tcgctgtcgc cctgatagta cacgtccttg ccgtcagcgt cccaggtgag gcgttcgccg
    10441 tcccgttcaa aggtggcgtt gccctccacc acggtcagnn nnnnnnnngc gggtcgcgtc
    10501 ggtcagttca tccagcttat ccgggttctt cagccagtcg ctgacgatga tccgccgggc
    10561 ctgaccctgc gcgtcggaga gaatgtacac ggtttcatcc tcggcggcag cttcggccat
    10621 cgccgggacg gcggcgcaaa gcagcagcgc cagagaaaga accagcgcgg aaatgcgctt
    10681 ccaattgctt ttcatggtga agatctcctc cttagttttt cactttcatg cccagcgtgg
    10741 tcttgcagac gaccttgtcc agcagcagaa gcaaggcggg caggaacagc agtacgctgc
    10801 ccatgctgac cagcgcgccc cgggccatca gcatgcacat ggagcggatg atgtccatat
    10861 cggagtacag agccacgccg aaggtggcgg cgaacaggcc cagaccgctg accagaatag
    10921 agggcgtaga cgcgcccagc gcggtggtca cggcttcccg tttggcgctg ccgcccacgc
    10981 gctccgcctt atagcgggtg gtcatgagga tggcatagtc cacggtcgcg cccaattgga
    11041 tggtgctgat gcagatgggg gcgatgaagg gcagggactg gcccagatag tgaggcaggc
    11101 ccagattgat gaagatagcc agctcgatga ccgcgatcag gatgaagggg agcgtcaggc
    11161 tcttttccac cagcaggatg atcaggaaca cggcgatgat ggacacggta ttgaccacct
    11221 ggaagtcgtg atcggtggtg tcgatcatgt ccttcatgca gggtgcttcg ccgatgagca
    11281 ttccggaggg gtcgtatttc ttcaggatgg actgaaggtc ggtgatctgc tcgttcaccg
    11341 cgtcgctggc cacccggtac tcggagctga tgagccacag ctgccacttg tcgctttcca
    11401 cgatgttgcg cacgctgtcc ggcagcacct ccgcgggcac ccggctgccc agtacgctct
    11461 gcagactgag cacatagcgc acgccgtcca cgttctccat ttcgtcctgc atggcccgca
    11521 gttccttgga ggacaggctg gtgtccacca gcaccatgtg ggtggaggca atatcaaagt
    11581 cgtcgatcag cttatggttg gcgatcacgt agtcgatgtc ctccggcagg cattgaccca
    11641 tatcgtagta gacctcgtcg ttggtcttct ggtagccgta cagggccgga tagaccagca
    11701 gcgcaaagat caccagcatc acggggaagg ccttggtgat gcccttggca aagccgttgg
    11761 cgttgcggat cagcggacgg tgacgggtct tctgcagggg gcggtccagc agcaggatca
    11821 gcgagggcag cagggtgacg cagccc
//
LOCUS       DAWAEA010000007        11576 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_891_length_11576_cov_2.450655, whole genome shotgun sequence.
ACCESSION   DAWAEA010000007 DAWAEA010000000
VERSION     DAWAEA010000007.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 11576)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11576)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11576
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_891_length_11576_cov_2.450655"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    531..540
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2170..2179
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3464..3473
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3788..3797
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6620..6715
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7048..7057
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7920..7929
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2260 a         3349 c         3603 g         2208 t
ORIGIN      
        1 gacccgcaca acggcgacct gcagggcaac ctttacgcgc cggatgtgac catcgggccg
       61 gacgggcggt attatctgta ctacgtgctc agcaatgtga atttcgtatc cgtggcggta
      121 tgcgacaccc cggcgggggc gtatcagttt tacggatatg tacactaccc ggacggtacg
      181 ctgctgggac ttgccccggg ggacgaggcg cagtttgacc cggcggtgct gacggagaac
      241 ggaaaaacgt atctttatac aggcttttgc ggcgtgggcg acccgaataa gcacggggct
      301 atggtcacgg tgctggaagc cgatatgctg acggtgcggg aagcgccggt ctacgtggtg
      361 cccagcacgg ccaccagcgc gggaacgggc tacgagggcc acgagttctt cgaagcgtcc
      421 tccatccgca aaaaagacgg gctgtactat ttcatttatt cgtccgttaa gttccatgaa
      481 ctgtgctatg ccgtcagcag ggaacccacc gggggctttg tttatcaggg nnnnnnnnnn
      541 gtgtgctggt tagcagcggc gatctgaaca tcgataccta caagcccgcc gaaaaatgcg
      601 cctactacac cgccaacaat cacggcagca tggtggagat cggcggcgag tggtatattt
      661 tctatcaccg tcacaccaac ggaaccaatt tcagccgtca gggctgtttg gaaaagctgc
      721 attttcgggc ggatggtacg gcaccgcagg cggaaatgac ctcctgcggc ggtactcccc
      781 tgcggggcga gggcgaatat ccggcttatc tggcatgcaa tctgttcacg gacgtagaaa
      841 agacctacgt cccctggtct ggctggatgg acgaccgctt ccccaagatc acgcaggagg
      901 gccgggacgg cgacgaggtc tacggctaca tcgccaatat gtgcgatcac gccaccgccg
      961 ggttcaagta tttcgactgc cggggcgttc ggcgcgtcag cgtgcgcacc aagggctttg
     1021 ccggcggaca gctggaactt cgcacggcat gggacggcga accgctgggc agcattccga
     1081 tcggctacgc caacgtatgg cacgacacgg cggcagacat tgccattccc gacggcgtgc
     1141 acgcgctgta tttcaccttc gcgggggaag gtcatctgca atttgcgtcg tttacgctgc
     1201 tgcgcgggga tgaataatgg aaaaagaaag agccggggga aatcctccgg ctcttcattt
     1261 gccaaaaaat ggagtggcaa gactgcctac agggagcccc tcaaacgtat gccacgacgc
     1321 gtcccgtagg gagaaaagag gcgcttcgca gctcaccctc cataggggct gttcacggac
     1381 aatgacgtcc tttttctgtt aattggcctt tcggagctta ccgatcagcg tgaacagcag
     1441 gaaggccgcc acgttggcga cgaccacgct ggctcccgtg gggacggaca gggcgaagga
     1501 cgcgcataat ccggcgaaaa agcacaccac ggaaacggcc agcgcacaga gcagcacctt
     1561 gcggaaactc ttgaacaccc gcatagccgt cagaccgggg aacacgatca ggctggagat
     1621 cagcatggcg cccatcactc gcatccccag caccacggtg atggcggtca gaaaggccag
     1681 cagggcgttg tagcgctcga ctttttcgcc ggtagcccgg gcgaaggtct cgtcaaaggt
     1741 cacggagaaa attttccgat agaagatggc gaacagactc aacaccaccg ccgccagcac
     1801 cacgctcagc ttcaggtcgc tttcgctcat ggccagcaca ctgccgaaca ggtagttgtt
     1861 cacgtcgccg gtggtgccgt tggacaggga gagggcgatc acgcccacgg ccagcgcggc
     1921 agtggagatc atagcgatca tggcgtcgcc tttgagcttg ccgcggctgg acagacgcag
     1981 cagcaggaag gcggccacca ccactacggg aacggcgacc gccatggggg caagccccag
     2041 cgcggaagcg acggccagcg caccgaagcc cacatggctc aggccgtcgc cgatcatgga
     2101 gtagcgcttc agcaccagac atacgcccag cagcgccgcg cacagggaca ccagcaggcc
     2161 cactacgccn nnnnnnnnnc gctcggacca taaaccgata ggaaaacatt tcgaccagag
     2221 cgtcaatcat ggctgcaacc tcccacaaag cgctgcccca ggggggaatg gcggtattcc
     2281 tcgggcgtac ccaggaaaat gccgtcatga gaaagatgca ggatgcggtc gccctgacgg
     2341 agcgccgcat ccagatcgtg ggagatcatg accacagtca accccttttc ccggtgcagc
     2401 atatcgatgg tctggtacat ttcccgggtc accatgggat ccagccccgc cacgggttcg
     2461 tccagcagca gcatggtgcg ggtggcgcac aaggcccggg ccagcagtgc acgctgctgc
     2521 tgcccgccgg acagttccgc gaagctgctc tttttgcggt cctccatgcc catcagcgca
     2581 aggtttcggt cgatcagctc ccgctgagcc ttggtgggga acaggttcag cccccggcag
     2641 ccggaggaga ccacctccca caccgaggcg ggaaaatcct tctgggcctc ggtgcgctgg
     2701 ggcagatagc cgatctccgt attatggaaa ccgggttcat aggtgatctt tcctttagcg
     2761 ggagcgatca gccccagcaa acccttgatc agggtggatt tgccggagcc gttttccccg
     2821 acgatgcaca ggtattctcc ccgttccacc gagagggaaa gatcctccag caccgtgtgc
     2881 ccctcgtagg cgaaggagca atggtcgcag gtaatcagag ccatgtcagt tcagtgcctc
     2941 cttcagcgcg tccgcgttgc ggttcatcag ggtcagatag ctggccccct cgtccatttc
     3001 ctgctgggac accgtgtggc agctgtggaa cagcagctcc ttcgcaccgg tctcctcggc
     3061 caaaatgtcc gccgttttgc ggctggaaaa ctcaatgtag aaaacgaccg ggatctgttc
     3121 ttcccggatg gcgcggatca gcgaaacgat ggtcttcacg ctgggctcac tgtcctcgct
     3181 gcagccagga aaggccgcgt catagcgcag gccgtaggcc gccgcaaagt agcggaaggg
     3241 gaaacggtcg ccgaaaacga ttagattccg cttgccctcc gccacgatgg tctcgaagcg
     3301 ctcgtccagc gcttgcagct tttgctgata ggcgacttcg ttttgggtaa agcgttcggc
     3361 gttttcaggg gaaagagcgc acagcttttc ccgtagggcc cggacgatca gctgcacgtt
     3421 tttgggggac gtccagacgt gctcgtccat ttcctcgtgg ctgnnnnnnn nnngtcttcg
     3481 tcgtggccat ggtcgtgctc cggggccatg ctctgggaat gctcctcctc cagcagggta
     3541 acgcagtccg ccatggcgaa ggtctccggc gcgtcgtccc ccagcgaagc cagcacccgt
     3601 tccagccaag tgtcgctttc gccgccggta tagaggaaca ggtcagcgtt ttgaatggcg
     3661 atcatatcct gcggggtggg ttcataggaa tgagcgtccg ccccgggcgg cagcagcagc
     3721 gtgacctccg ccgcgtcccc ggcgatgacc cgggcaaaat cgtaggcggg aaagacggcg
     3781 gctaccannn nnnnnnncga cgattccgcc cgtacaggcg aaagggaaaa aagcagcgca
     3841 atagcgcaga aaaaggcagc aatccgacga atcaatgggt ttcctccttg ttttgacagg
     3901 cggcgcacag gccgtagagc agcgtccgtc cctcgtccag cgaaaagccg tgatgatggg
     3961 acagatgctc cttgaacagg tcgatctcgc cgcagtgcag gtgctccata gcgccgcagc
     4021 gcacgcagcg gatgtgcagg tggctttccc ggcagcggtt gagctgataa tgggccgatt
     4081 cgccgctttc ctgcggggcg tagcggcgca gctccccgct ttcacacagg cgggtaatgg
     4141 cgcggtacac ggtggtcttg ccgatggcca gcccttcccg gctcaactct ttttccagct
     4201 cctgataggc ttcctccgcc gtcaggcagc tgtcgggccg gtttcctgct gacgggtctg
     4261 ataggaaccg cgggcagaca tggcgcaacc tcctttattt tgcttatttg aaaatgaaat
     4321 tcattttcaa aacggtatta taaggacttt ggtctgcgtt gtcaagtcaa aagttagcat
     4381 cggataattc ccatgcattg caattcgggc cggggtgcgc tatacttacc acatcccttt
     4441 cggatgtttt caccggtgtg ttttggccgg ggcaaaagca tccttcctga acgacagaaa
     4501 tgaaacccga gggaatctcg aagaaagcga ggcgagcaga aaaatgcgta tttctatgat
     4561 ctgtatcggt tccaccggcg acgtgcgccc ctatatcgtg ctgggacgcg aactgcagcg
     4621 ccggggccat caggtgacga ttaccgcttt ttccgatttt gaaaagacga ttaccggcga
     4681 agggctgggt ttccacggcc tgtccggcaa tgtgcgggaa tttatgggca atatcatgaa
     4741 gcccggcgtg aacggtgtgg cgtatctcaa tcaggtgcgc aactccctga aggatgtggt
     4801 ggatcccttc atgaaggatc tggaggacgc caccgccgag tgcgactgca tcatcggcac
     4861 gttcttcggg caggtgatcc gctccatggc ggaactgcgc cacctgcctt ttatccagac
     4921 ccagtatttc gtgatggact ataacaccga cgcgcccatc gcttccgccc ccggccagcg
     4981 tatgggcaag gcgtggaacg cggccagcta tcatctgggc tattttctca tcagcacgat
     5041 ggaaaagtac tatctggacg attggcgcaa ggagcacggc ctgccgcccc gcaagctgga
     5101 aaataaggct tcctatgaac tgaacggcca taccattccc gtgctgtatg cgctgagtcc
     5161 gctggtgatg ccccggcctg cggattgggg cgcgaacatc catatgaccg gctactggct
     5221 ggcggacaat ccgcaggaat acaccccgga gccggggctg cagcagttcc tttccgaggg
     5281 cggaaagccc atttatgtgg gctttggctc catggtcagc ggcgatatgg gcgaaacgct
     5341 ggagatcgtg ctggaagccc tgcgcatgag cggcattcgg gccgtgctgt ccaagggctg
     5401 gggcggcggc gaactgccgt cgaacctgcc ggataacgtc tatgtggcgg gcttcgtgcc
     5461 ccatgactgg ctgttccggc gagtacgggc cgtggtgcat cacggcggtg cgggaacgct
     5521 ggctgccggg ctgacggccg ggctgcctac gctggtcatc cccttcggcg gcgaccagcc
     5581 cttctggggc agccgggtgc aggcgctggg cgtcggccct aagccgattc cccgggagcg
     5641 cctcaccgcc cgcaaactga gccgggcgct gatcgagctg accagcgaaa agcgctatga
     5701 ggtagcggcc cgggagttgg gtatccgtct ggaaaacgag gacggcgcgg tgaacgccgc
     5761 caacatcatt gaacacgaac tgcgcaaata cctgcgccag gagggattgt cccctgtgct
     5821 ggtgcgcccg gaggagcatt catgagcgaa acgaaaacca tggcggatct gactcccggt
     5881 gaaagcggcg tggtggacac ggtgggcggc gaggacgcgc tgcaccggca cctgctggat
     5941 atgggcatta cccccggcgt gaaggtgctt ctgcaaaaaa cggcccccat gggcgacccc
     6001 atcgagatcc atctgcgggg ctatgcgctg accctgcggt tggatgacgc acggaagatc
     6061 acgttgaaat aacggcgctt ggacggcttt gccgggacgg cggcacgaag aaacgggctg
     6121 ccgccctgtt ttgacgaaac cgtcattttc aggcttttag ctgcgcattc caaaggaaag
     6181 gcgaggtttc ctcatgcagt atactttcgc gctggcgggc aaccagaaca gcggcaagac
     6241 gacccttttc aatcaactga ccggctccaa tcagcacgtg ggcaactggc ccggcgtgac
     6301 ggtggagcag aagaccggca ccatgctgca ccatcaccac ggcaagcaga cccacatggg
     6361 ccttccctgg cgggtgcgcc gggagcagca ggagggcaag ccggtgcccg atcagtacag
     6421 cgacgtgacc atcgtagacc tgcccggcat ctattccctc agcccctatt ccatggagga
     6481 gatcgtctcc cggaatttca tcgtcaggga tcagccggac gtggtcatca acattgtgga
     6541 cgccaccaat ctggaacgca atctgtacct gacgctgcag ctgctggagc tgggccggcc
     6601 ggtggtggtg gccctgaacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngggcg
     6721 agggcatcga cgaactggtg agccgggcta tggaggcggc agaaaacaag accgtgcccc
     6781 cgaagttgga catctgtgac ggcgaggccc atacggcgct gcacgccatc gcccatctgg
     6841 tggaggacga agcgaaggag catcacatgc agccccgcta catcgccacc aagctcttcg
     6901 agggcgacaa gccgctggaa gaagaactgg gtctgagcga ggagacccgg cacatcatcg
     6961 aggaaattct ttcctccatg gaaaaggccg tgggcatgga gcgcgccgcc gtcatggcgg
     7021 acacccgcta ccgcttcatc gaaaagcnnn nnnnnnnaaa agctgctgtc cacctgcttt
     7081 aagcggggac aaacggatga aaacacgctg accgaccgca tcgacaacgt gctgacccat
     7141 ccgctgctgt ccattcccgt gttcctgctg atgatgtttc tggtgttttt tctcaccttc
     7201 gggccggtag gcagcttctt tgccgatgga ttcgctctgc tgatcgacag cggcgtgtcg
     7261 ctgctggcgg gcgctctccg caatgcgggc gtggccgaat gggtgcagga cctggtcgtg
     7321 ggcggcgtgc tgacaggcgt aggcagcgtg ctcaagttcc ttcccaccat cgtgatcctg
     7381 tttctactgc tgtccattct ggaggacagc ggctatatgg cccgggcagc tttcctgatg
     7441 gataagcccc tgcgcaagct gggcctgaac ggccgcagct tcatccccat gatgatgggc
     7501 ttcggctgca ccgtgcccgc cgtcatgagc gcccgaagca tgaacaacga ccgggaccgc
     7561 cggtttacca tcatgctgac cccttttatg agctgcggcg ccaaggtgcc tatctacgcc
     7621 ctgttcactc gggcgttctt cccgggaaat caggtgtggg tgatgaccgg tatgtacctg
     7681 atgggcatcc tcgtcgccat cggcgctggg ctggtgctga agcgcaccgt gttccacggg
     7741 gacgcggctc ccttcatcat ggaactgccc gcctaccgtc tgcccacccc ggccaatgtg
     7801 ctgcggcaga tgtgggataa ggcgaaggac ttcctgcagc gggcctttac cgtgatcttc
     7861 ctggcgacgc tgatcgtctg gttcctgcaa aatttcacct tccggctgga aatgacctcn
     7921 nnnnnnnnng ctccgtagag gcctccaccg ccgtgatcac cggcctgatg gccaaggaaa
     7981 gcgtggtcag cacgctggcg gtgctgagcg gcgtggacgc ggaaagcgcc cagatggttt
     8041 cagtgatgag acagattttt ccctcaacgc tggcggcggt gtcctttctg gtgttcgtgc
     8101 ttctgtatat gccctgtgtg gcggcggtgg cggccatgcg ccgggaaatg gaaagcagcc
     8161 gctgggccta cggaactatt gccgcccaga cggcgctggc gtggacgctg gccacgctgg
     8221 tgttccagct gggccggctg ctgggactgg gctgatttcg agatcagccc tgcaaatttg
     8281 cagaatggcg ataaggttca gcgccccgca gggatgaaaa acagaatggt ttgaaatacc
     8341 gatggcacgg gccgcaacaa gggtgcgaag caccgagggg tgaggacagc ggcgaagccg
     8401 ctccgcagcc ccgaagcccg cgaacctgta gaaggcgctg gctgcatccg ccgatggttc
     8461 ccgctttcac tgaaaggaaa aaatatgggc acagtactgg acattttgat ggccggaaaa
     8521 tgcgccaaag gcacaaggga ggaagaaaaa catggctgaa accccttccg tcatccgcct
     8581 gtttgggctg gtgacggatt ccattgtaga cggccccggt ttccgggtgt ccatttttac
     8641 gcagggctgt ccccatcact gcgagggctg ccacaacccc ggcagccatg acccggcggc
     8701 gggcaaggaa tggacgctgg acgatgtgga aaagaaattc accggcaacc cgctgctgga
     8761 cggcatcacc ctgacgggcg gcgagccctt tcagcagccc gcagcctgtg ccgagttggc
     8821 ccggcgggcc catcagaagg ggctgaacgt gtggtcgtac agcggctaca cctatgaaaa
     8881 gctgcgggag atggcccgcg gggacgaggc ggtcaacgcc ctcctttcgg agattgacgt
     8941 gctggtggac gggcctttcc tgctgaagga gcggtcgctg gagctactgt actgcggcag
     9001 caaaaaccgg cggatcatcg acatgacaaa aacccgggag acgggcgaag cggtgctttg
     9061 gacgcccccg gaatggtaaa tggaataaga aaaaagcggc tctgtttttg ggagagccgc
     9121 ttttgtttcc ataaaaacgc ccccaaggcc tttctgtgtg ggaaagtggc ttcgggggcg
     9181 ctgtgcgtta ttcttcgtcc gagaatttca gcggatagga ttccaccgcg tattccagct
     9241 ttctgccgtt ttccttcagc acccggagca ccgcctcgat gtgggtgtcc ttggtgtagg
     9301 gagtgggggt gaaatcgttg tagtcggtgc ggctggaaat gcggcaggga atgatcttga
     9361 attcttcgct gctggccacg ccgtcccgga cgcgcacccg gagctggaaa atataggagg
     9421 acatatcgtc cggcttatta ttgcccgcaa agctgaaatt acccagcgaa tagcagatgt
     9481 acttgccgtt gtagtattcg atggggttga tgcggtggct atggtggccc accaccagat
     9541 cggcgcctgc gtcgatggtg gcccggccca gccgaacctg attgtcgttg ggggaatagt
     9601 ccttttccgc gccccaatgg tagctgacca cgatgaactc gcagccctgc gcccgcagcg
     9661 cggcaatatc ggccgggact ttttctatca gctcgtcgtg acggccgttg aaggtctgat
     9721 aggccagaga gccgatcttc acgcccttca cagtcatgat ggcgggttcg tcctcgctgg
     9781 cgtaggggat cccggcggcc agcagggtct ccttggtctc cttcaggcct tcttcgccca
     9841 tgtcgtcggc gtggttgttt tcgaaggaaa cggtgtcgat gccgcctgcg ggcagcatgg
     9901 cgacgtagct ggggtcggcc cggaaaagga aggaataatc ccgttttttc gggtttttcc
     9961 cggcagtggt cagcgtacct tcgaagttga ccatggtcat atcgtccgcc agaaagatat
    10021 cccggacgtt gcggaagggg aaggtaatat cgcccttctg ctttttcagt tccttctcaa
    10081 agatggagga gctggcctgc acgttagtgc cgatggtcac gtcgccggtg aaggacaggg
    10141 tcagcagaat ggaaccgtcc tgctcgtaga cgggcaggga agggtcgcgg gtgggctcgg
    10201 gggtttccag gggctcggcc tcgtcctcgc cgccctccgg gggaacgggc gtgccctcgc
    10261 ccgccaccac gttgccctga ttgtcgtaga tggtgatgga aacgtcctcc tcagccagcg
    10321 caaacgcgcc ggccagcagg gcgaagatca gaagaaaaga taagacacgg aagaatcgcc
    10381 ggtgatccat cggacatcgc tcctttcggg tcggaggaat ttcggtcaca gccggtcaag
    10441 gaagcgcttc atgcgctcca gcgcctcgtt gagctgagaa agcgccgtgg cgtagcagca
    10501 gcggatgtgc ccttcgccgc tctggccgaa ggccgtgccg ggcacgcaga ccaccttttc
    10561 ctctttcaga aggcgggtgc agaactcctc gctggtcagg ccggtcttct ggatgccggg
    10621 gaacacgtag aacgcgccca gcggctcgaa gcagctcagc cccatatccc ggaaggcctg
    10681 caccatcagg cggcggcggc ggtcgtagct ctggcgcatt tcgatcacgt cccggcagcc
    10741 gtcctcccgg gcccggcgca gggcctccag cgcggccacc tgtccctgac ggggcgcgca
    10801 catgatggcg tactggtgaa ttttgcacat cacgtccgtg aaaccggctg gggcgcagag
    10861 atagcccatg cgccagccgg tcatggcgaa ggccttggag aagccgttga ggtagatcgt
    10921 tcgctccttc atgtccggca gggacgcaat gctgacgtgt ccctgaccgt tgtaggtcag
    10981 ctccgcgtaa atttcgtcgg aaatgacgat caggtcatgc cgcaggatga cttcccgcag
    11041 cttttccagc tccgggcggc ccagaatgcc gccggtgggg ttgttggggt agggcaggat
    11101 cagcgccttc gtcttggggg tgatcgcctt ttccagcgcc tcgggggtca gcacgaagtt
    11161 tttggcttcg ctggtgggca cggccaccgg gctgccgcct gcgaagatca cgcagggggc
    11221 gtagctgaca tagctgggtt ctggcaccag cacctcttcg ccggggttga gcagcgcccg
    11281 cagcgccaga tcaatgccct cgctggcccc taccgtgacc agaatttcat tcttggggtc
    11341 gtaggaaata tggaaacggt tgtccacata ggcggcaatc tcctgccgca gttccagaag
    11401 gccccagttg ctggtgtact gggtctcgcc gtccagaatg ctgttgatgg ccgcgttgcg
    11461 gatgtggtag ggcgtgacga agtccggctc gcccacgccc agcgagatcg cgccggggat
    11521 ggtggccgcc acatcgaaga atttacggat gccggatttg ggcaccgccg ccacgc
//
LOCUS       DAWAEA010000008        11050 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_945_length_11050_cov_2.912597, whole genome shotgun sequence.
ACCESSION   DAWAEA010000008 DAWAEA010000000
VERSION     DAWAEA010000008.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 11050)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11050)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11050
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_945_length_11050_cov_2.912597"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5767..5776
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2285 a         3206 c         3520 g         2029 t
ORIGIN      
        1 ggcggagcca ttacgctggg catgatcgct tcgttcctga ccctgtcccg cagcttcgtc
       61 atgcccatct cgcaggtgag ccagcagctt tcctccgtca tcatggcgct ggcgggcgcg
      121 gagcgcatct tccgcctgat ggacgaaccc agcgagaccg acgaaggcta cgtcacgctg
      181 gtgaacgcca agcgggatga gaacggccag ctttgcgaaa gcgaccatcg caccggcctg
      241 tgggcgtgga agcatcctca cagcgacggt accaccacct acaccgagct gaagggcgac
      301 atcgtgctgg acgacgtgga cttcggctac acgccggaga agatcgtgct gcacgacatc
      361 agcatttacg ccaagccggg ccagaagatc gcctttgtcg gcgccaccgg cgctggcaag
      421 accaccatta ccaacctgct gacccggttc tacgacattg ccgacggcaa aattcgctac
      481 gacggcatca acatcaacaa gatcaagaag gacgatctgc gccactcgct gggcatggtc
      541 ctgcaggatg tgaatctctt caccggcacg gtgatggaga atcttcgctt cggcaacccg
      601 gacgccacgg acgaggaatg catcgaggcg gcgaagctgg tcaacgcgga cggcttcatt
      661 cggatgctgc ctcagggcta cgacacggtg ctgaccggcg acggcagcgg cctttcgcag
      721 ggccagcggc agctgatctc cattgcccgg gccgccgtgg aggacccgcc cgtgatgatt
      781 ctggacgagg ccacctcctc catcgacacc cgcaccgaga gcattgtgca gaagggcatg
      841 gacgccctga tgaagggccg cacggtcttc gtcatcgccc accggctgtc caccgtgcgc
      901 aactccgatg caatcatggt gctggatcat ggccgcatca tcgagcgggg cagtcacgcc
      961 gacctgatcg cccagcgggg cacctactac cgcctctaca ccggcgcctt cgaactcgaa
     1021 taaggcagtg ccttaagccg gactgcgcgc ctacggcgcg cagggtgctc gtgggacatg
     1081 cggcttggcc ggggttttat cgcgggtctg gcagggtttc cagccagctg gcgtgtacag
     1141 gccgccattc ggcggaaggc ctcatgactt acaaaaagac cagcatggtt tttgttccat
     1201 gctggtcttt tgttatgcat ttggggtcag gcgacggtat gcttgacgcg gtcatgctcc
     1261 tcggcgcgct tggcgttatt gaagcgatcc agcgtaccca cgaggtagcc ggtgatgcgg
     1321 cggatgcggt ggaagggctg cgcgcccaga tcgtaatcca gttctacctc gtcgccgcag
     1381 accttgatgt cgatatggcg gggttcatgg ccgaactttt tggtggcacg gtcgatatat
     1441 gcctggattt caggctgttc cagcgttccg ttgctgacag taacagtaca agccatagta
     1501 tgacgctcct ttatatcacc cagtcgggca caaaatatgg ttatttggac gataaatagt
     1561 ataccacgtt ttcggagggg tgaaacacaa aaaagaactt tataaatggg ggataggtca
     1621 ttagaaaaag caatggaatc tttccaaacg ccgccgcata ggcttttgca aaaccaatgg
     1681 ggaggggaac ggcgtgagcg aggtgcagag cattcagctt cccgcggcgg ggagcgtcac
     1741 cagccagacc gcgtccaacg gctggcgcag cctgctggaa agcaacatgg ggcggagagt
     1801 cgtggcggaa tttttgatcg gtcaggacgg catggcggaa aaaacggggg aaatttacgc
     1861 ggtgcagtca gattattttc ttttgaagga cgataacggc aattatgtgg ccagcgacct
     1921 gttcagcctg aaattcgtta ccttctgtcc gggctgcgaa acggccgccc agtccggcac
     1981 ggcagcacaa agcgaaactt cggcggatag cggcgcgtcc gccatggtgg taacgcctct
     2041 gcgcggcacg gcccccgcgg cggcgctgaa ttacgccaaa cggaaggccc gccggtaagc
     2101 ccaaaatcaa cagccgtttt tctccgacgg atgaaaccgc gcccgccgga gaaaactggc
     2161 cttgaaatcc tgctcgaatc gcgttatact gggggacgat tcaatacatc ttatccaaag
     2221 gagcaaatgt atggcaagac tgttcggaac cgacggtgtg cgcggcatcg ccaatacgga
     2281 acttacctgc gatctggcct tccggctggg acaggccagc gcctatgtgc tggccagcga
     2341 cgttcaccgg cccacgatcc tggtgggacg ggacacccgc atcagcgggg agatgctgga
     2401 aagcgcgctg gtggcgggca tttgcagcgt gggcgcgcgg gcggtgctgg tggaggtgct
     2461 gccgaccccg gccatcgcct atctgacccg gttctacgag gcggacgcag gcgcggtcat
     2521 cagcgccagc cacaacacgg tggaatataa cggcatcaag ttctttgaca aaaacggcta
     2581 caagctgccc gacgcggtgg aggacaagat cgaggagatc gtccgcaacg ggatgccggc
     2641 ggactttgaa gcgcccaccg gcgagcgggt gggtcgtccc gtgcggcagc gcaacgccac
     2701 ccggcgctat gtggacttcg tcaaggagac cacggatgtg cgtctggacg gcctgcacgt
     2761 ggtgctggac tgcgcgcagg gggccagcta tgaagtagcg cccatggttt ttaaggaact
     2821 gggcgcgcag gtcagctgct actatcacga gcctgacggc accaacatca acgatcactg
     2881 cggctccacc catccccgcc gcctgtgcga gctggtgcgg gagctgggcg cggacgtggg
     2941 ctttgccttc gatggcgacg cggaccgcct gatggccgtg gacgagcggg gctctcttat
     3001 caacggcgat caggtcatgg ccatgctggc cgtctatctg aaagggcagg gacggctgcg
     3061 gcagaacacc attgtggcga ccatcatgag taacatgggc ctggatatcg ccatgaagaa
     3121 gcacggtatc cgggtggaaa agaccaaggt gggcgaccgc tacgtgctgg aaaagatgct
     3181 ggcggacggc tttgtgctgg gcggcgagca gagcggccac gtgatcctgc tggatcacaa
     3241 caccaccggc gacgggctgg tgacggcggt gcaggtcatc agcgccatgg tgaaggccca
     3301 gcgccctctg agccgcctga gcgcggtgat gcagatcatg ccccagagcc agtacgcggc
     3361 caatgtatcc gacagccgga agtatgattt cgacaaaaat gaaaaaatca tggccatgat
     3421 ccgccagctg gaagaaaagt acaaggacaa gggacggctg gtgatccgtg ccagcggcac
     3481 cgagcctcgt gttcgggtga tgatcgaagg ccccgaccag cgggagatgg atcgggatgt
     3541 gtacaccctc agcaagctga tcgaaaaaga actgaattga ttttccgcac gtcaggagct
     3601 tcccggagca gcgttccggg ggctcctttt tttgcgcctt ccaacgttca caaaccgttt
     3661 atttctcttt ttacgcaaag tatggtataa tacaaaatgt atgattctat catgcgttca
     3721 ttatcgctgt ttgtttggag ggctctggta tgttcgggaa gctgatgaac cgctatttct
     3781 acggaaagag cggacaggga gattttgaaa aggaagacct gccccaaaac cggtggcagc
     3841 tgttctggga gatgctgcgg gtgcggtttt ccgcgctttt gcggctgaat ctgatgtatg
     3901 tggtcgtgtg gattcccgcc atcttcttca tcggccggtt cctgatgtac ggctattccg
     3961 ggctggtgaa tctgagcgat tttcaggcgc agctggaggc cggctccatc accgccgagg
     4021 cctatcagga gaatttcgcc ctgttccagg aaggaatgcg gtcgctgctg ttttccacgc
     4081 tggtgttcct gattccctgc atcggcatca ccggccccgc caccgccggt ctgtgctatg
     4141 tgacccgcaa ctgggcccgg gacgaacatg cctttatctg gtcggactat aaggacgcca
     4201 tcaaggccaa ctggaagccc gccctgctca acagcttcat caccggtctg gtgcccgtca
     4261 tgctgtatgt gtgctgcacc ttctacggct ccatggcgaa aagccagagc ggggtgttca
     4321 tcgtgcccga ggtgatctgc atcatgatcg gcgtggtgtg gctgatgatg cagatgtacg
     4381 tctatcccca gatcgttacc tacgacctga aatacaaggg cgtgctgcgc aacagtctgc
     4441 tgatggctat cggccgtctg cccatgacgg tgggtctgcg gctgctggcg ctggtgccta
     4501 cgctgatttt cgctgtcgtg tcgctgctga ctccctactt ccagtacgcg gtcatgatct
     4561 atgcgctgta ttacatcctc atcggcttca gtctggatcg cttcatctgc gccagctaca
     4621 ccaacggtgt gtttgacaag ctgatcaacg tcaagatcga cggcgcgcag gtgggccgcg
     4681 gcctgtacgt ggaggaagac gacgacgagg acaaggaaaa cgaggaaggc gcagaagccg
     4741 cggacgaacc ggcggctctc ccggacgaaa acaacgctcc ctccgaaaaa gaatgaacca
     4801 tgcatggacg gctccgccct tcggcaagct atgatcgaag gagggagcgt ctatgaacca
     4861 taaacagggc tgggcgtccg gcgcaatgcg tcggaacgcc tttgccttcc ttacccggca
     4921 ggagcgccgg aagtatgaga tcgcccggga gctggggctg ctggaccgcg tgaaggccgt
     4981 aggctgggcc gggctcagcg ccaaggaaac gggccgcatc ggcgggctga tgggccggga
     5041 ggcccgcgca gcgccgtcgg cagtgccgcc acaggcagcg ccggaatctc cggcagcgcc
     5101 gaatccgcag gcgacaaaag aaggagaatg aacgcatgta caccgcattt gcatccgtat
     5161 acgaccggct gatgaccggc gtggattatc agggctgggc gaatttctat cacgccctga
     5221 tggagcgcta cgggattccc cggggaaagg tatgcgaatg cgcctgcggt acgggcagcc
     5281 tgaccattcc gctggctgcc atgggctacc agatgacggg ggtggatatc agccccgata
     5341 tgcttttcga ggccagccag aaggcccgca aggccggggc gatgatcccc tttgtaaagc
     5401 aggacatgcg ccagctgcac ctgcaccggc agatggacgc ggtgctgtgc acgaacgatg
     5461 gcgtcaacta cctgcgcagc gaggacgagc tgcggcagtt ctttcaggcg gcgcatcggg
     5521 tgctccggga gggtggcggt ctgtttctgg acttgagcac cccctacaag ctgcgccggg
     5581 tgctgggcaa tcatttcatc ggcgacgagg acgaaaatat cgcctacctg tggcagaacc
     5641 gctaccatga gcagcagaac tgtgtggaga tgaatctggc cattttcgtc cggcaggagg
     5701 gcgagaccta tctgcgcatc ggcgaacagc agcgccagta cgcccatacg gcggaaacgc
     5761 tggggannnn nnnnnnggac gctcctgcac gagtgcggct ttaccgacgt agctgttttc
     5821 ggcgataagc acatggaagc gccgggcgaa aaggaatgcc gctggttcgt tgccgcccgc
     5881 aagccgcttg gcggcgcgga agtctgattt ccgggaaagg aacggaagaa aaatgagcga
     5941 gatcaaggaa ctctataatc gcaaggatca gatgatccac tttacgctgg cggacggcat
     6001 ggtgcgcggt ctgctctgca cggcggtgaa taccgttgca aagggcgcgg ccatccactg
     6061 cgccacgccg gtggtggccg ccgcgctggg ccgcaccctg atgggcacgg ccatgatggg
     6121 cgcgatgctc aagggggacg aagcctccgt taccgtcacc atcgatggcg gaggacccat
     6181 cggcaagatc atctgcgtag ccgaccggga aagcgtccgg ggatgcgtgg acgtgcccaa
     6241 cgtagagctg cccctgcgca aggacggcaa gctgaacgta ggcatggccg taggccggga
     6301 aggccgtatg tccgtagtaa aggatctggg catgaaaaat ccctatgtgg gccagacggc
     6361 gcttctgtca ggagagatcg cggaggactt tgccgcatat tatgtgcaga gtgagcagac
     6421 ccccaccctg caggcgctgg gcgtgctggt cagcggcgac acggtgctga gcgcgggcgg
     6481 cctgctgttg cagacgttgc ccggctgccc ggacgatatc gtcagccagc tggagctgcg
     6541 cagcccgctg ttttccgata tcagccggga attgtgctat gactccgccg agaatctgct
     6601 cagccagtgg ttcgacggcc tgaagcccgt ggtgctggag actcatccgc tggaataccg
     6661 ctgctattgc tcccaggccc ggatggaaaa ggccctcatc gcactgggca aggacgaact
     6721 gaccagtatg attgaggacg agatcgacgg cgcggagctg gtctgccact tctgcaagga
     6781 aaaatatcat tttacgcagg atgacctgcg ccgtctgctg gaacaggcga cgcgctgaca
     6841 gaaaggagcg ggcatggctt ttcagaatca cgaggggcag gtgcgttcct tccagatgcc
     6901 ggcctatttc tgggtcaggc gggcggaacg gagccgggaa gagggccagc ccgtccgggc
     6961 gctgtcgctg cttcgtcagg cgactcacga ggagccggag gagcgggact ggcggctgga
     7021 atatgcctgg cagctcaccc ggctttcctg ctatgaagcc agcaaccggg aatgtctgga
     7081 tctgctggcc cgctggcccg ccgcgttttc cgctttcggc ctgctgacgc tgaacatgat
     7141 ggcgctgggc cgcatgagcg agtgcatgga tgcttactcc atctataccc agttcattcg
     7201 gatgcacccc gacgccatgc cggagtggga cgaggacgtt tatgaggtgg aggatacgat
     7261 gttctccatg ggcaaggagc cggagcattt tcaccggctg aacgccctgc tgcgcatcgc
     7321 gggcaagtgt atggctcgtg gagattatca gcgggcggga gagctgctgg ttcgttcccg
     7381 gcaaacgccc ttttatgcgc cgtccccgga acgggacagc ctgatcgcag cctatttgtt
     7441 ccacaccgcg ccgaaggaaa acgcactgga agccattcgc gtcgccctgc actccaacgc
     7501 ccgttcgtcc tccgtattgc tgtccatggc cctgctgctg cggcggctga acgacccggg
     7561 ctggaaaccc gcgctgctgg ccgcggcgct gtatgccaaa acgccgccgg aggaagcggc
     7621 ggtgtgcaac gcggcggatc ggttccagtg cgcctatgtg gccctgggaa tgctgcgccg
     7681 cagcctgaag caaacgccca cacggttcag cgccgcctat aacctgtgcg tgctgcagct
     7741 tcgccggggc gatctggcgg agggaacgcg gatggcgcat ctgctgcggg agctggaccc
     7801 gctggatcgt caggtggagc ggctgtttgc caccgtgcgc cagctggaag aagcccaggc
     7861 ggacgaaacg gaagtccgcc gtgccgggaa gcagcagtgc ttttacggcg ttcccgacgg
     7921 cgtgatcgtg gcggaggacg tcatggagtt ttccagccgc ctgaccgatc atctgggcga
     7981 ttttttcacc gccctgcggc aggacgaacg gctgcgggga cagcttttgc gggtgctgca
     8041 tctgcacccc gacctttatc acatgatccc cgccctgtgc gaggaaatgc cggagctgga
     8101 cgctcagacc ctgctgcgtc gtctgctttt agacgagccg gaaagcatgg agatcgtccg
     8161 ggtgctcggt aatctgctgg agcactacgg ctgccagccg ccttatctgg tgcgccggga
     8221 cgggcatctg acgctgttcg acccctatgc cccggagccg gagcagccca ctttcctgca
     8281 aaggatgtgc ttccgccgtc tgcgggaaat ccgcagcgta ctgggcggct cggcattgcc
     8341 cagcgcgctg gccattcagg atcgcctgac ggaaagcgag cggaacgcat gggccggaga
     8401 tcggatgcac gtatgggcag cggcttttgt gacagtctat tcccagaagc acgggaatcc
     8461 cgtgccccgg ctggacggaa cgatgctttc gttccccgag cgctggaagg cgtatcgccg
     8521 ggcggtcaaa agcattcgca gagcatggaa tcagaaaggg agcgcaaact atggaagaga
     8581 agaatgaaaa gatggaactg atcgactttg acgacaagtt cacgcaggtg ctcaacgact
     8641 gggtggaaaa gaaccagacc aagttcgcca acgcggacga gatggaagcg gccatgccgg
     8701 acgtgtatct ccgcttcctg aatactcccg ccgactggtt gggcggcaag gcccccggca
     8761 acgcctttga cgatgtgagc gacgcggggg aattagtcgc cctgatgaaa cgttatatcg
     8821 aggaggaaat ccccgtgccc gatcctttgc tggatcgtat cgcggagatc gccgacgacc
     8881 agcccctgat ggagctggtg aaggacaagt ccgccccctg cgaagcccgc atgatgggta
     8941 tcgagctgct gcggcagatg gattccaccg cgcccatggt ggatttcctg cgctggcagg
     9001 tggagcggga ggacgaggac gatattctgg acaacgcgct ggaaagcctg cgtcagatgg
     9061 gcgagcaggt ggtcaagccc gccaagatcg ccttccatgc tgcggatgag gcgggcaagg
     9121 aagccctgct ggatctgctg gcggattatc ccggcgatca ggaggtcttt gacttcgccc
     9181 tgaaccagtt caagaacact aaggacaagc gtgcgctgta cgccggttat ctggccaagc
     9241 tggacgacga ccatgcgctg gaggccctgc tggacgtggc cgaaagcgac gatgtgacct
     9301 acatcgactt catcgagatt cgcagcgcca tcgagaggct gggcagcgag gccccggtgc
     9361 gggacttctc caacgatccc acctacaagg cggtcaagcg gcttcagctg aaataaccaa
     9421 cgatacccgg gggtgataca cgattgattt gtgaacgctg tggaagtttt cttcccgacg
     9481 acgcgctgac ctgcgaccat tgcggcgcgt ttctggggga taagggttcc tccgctatga
     9541 aagacacggg cgtgcgtgcc attcggcagg gacgctccgg ctcccagaag ccgacactgc
     9601 ccgtgcggca gggcggtatg cgggaatatg gagactatga tctttctccc ctgccgctgg
     9661 aaccggctga cagggcccag cgccgcagac cggtgcagcc catgagcgac accgtccgcg
     9721 gcagccgccc ggacacccgc aggggcgtgc cggtacagag cggccacctg cgggcgaatc
     9781 ccacgccccg aaaaagcgtg cgtctgcacc ccgtgcggcg gcacaacatc aactggatgc
     9841 tgatcggtct gatcgtggtg gtgctggggc tgggcgcgtt tatcggctac gaggtctaca
     9901 tggcccgcag cgacagcggc cagcgttcca cggctcggaa gaacgtgctg tccacctttg
     9961 aaccccagct ggagctggcc gtttccaagg acgcgacccg tacgacggaa aaggaagacc
    10021 tgcttaagca gtggaacagc gcgacggctc agtcctactg ggaagtggcg caggagtttg
    10081 ccgacggcgg cgatctggaa acagccatca ctgcgtaccg catggcggac attctcgatc
    10141 cggacaacta cgacggtctg ctggacatgg cctccaccta cgagatgatg aaccagaacg
    10201 acaaggcgga ggagctgtac ctgaacctga tcgaaaatgt cagtcccttc cgcagcgagg
    10261 cctacaccgc gctgatccga ctgtatcaga ataccgaccg gggccctgag gcggcggaaa
    10321 tgatgaagct ggcctacgag aacaccgacc gggaaacctt ccgcctgcag cgccgggaat
    10381 acattcccca aagtccgcag gtcaacctga gcgcgggccg ctacgacctg agcaagatgg
    10441 atcgggaagt ggatatttcc tcgccccaga gctttgatat ctactatacc tacgacgaca
    10501 acgccgtact gcctcaggac ggcatcctgt acgacggcgt cagcctgcac gcggaggaag
    10561 gcaccgtgac cctgcgggcc gtatgcgtta acggcacact ggtcagcgac gaactcagcg
    10621 tgagctacac cttctattat cccaccccgc ctgcccccaa gtgcaatctg gcccccaaca
    10681 cctacaagac cgccaagacg gtcagtttga gggccgggac ccgggaggaa tacaccaaaa
    10741 aagagcaggc ggagatggaa aagaatctca cctattacta caccatcgtc ggctccaccc
    10801 ctaaagccct gtttacgacg gcacgcccat tcagctgccc tccggcaagg tgacgctgaa
    10861 ggccatgtgc aaaaaccagt acggcaagct gtccagtatt ctggaggtgg gttataagtt
    10921 caacatcaag cccgcgccgc tggaaatgta cgccgagacg gacactctcg ccgatatggt
    10981 gctgctgacc accacccagc aggagtttga ggcggcgttc ggcagcccgc agcaggagat
    11041 cgacaccacc
//
LOCUS       DAWAEA010000009         9508 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_1170_length_9508_cov_2.537501, whole genome shotgun sequence.
ACCESSION   DAWAEA010000009 DAWAEA010000000
VERSION     DAWAEA010000009.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 9508)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9508)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9508
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1170_length_9508_cov_2.537501"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    252..470
                     /estimated_length=219
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7546..7606
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8372..8381
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1618 a         2871 c         2805 g         1924 t
ORIGIN      
        1 tctgctcccc cgcaggtggc gctgggcaga ctcaaatggc ggcgcttggg atgctccaat
       61 gcggctgaga tggtttcgca gcgcaggaaa acaggtttgt tttttgcggt tggcgctatc
      121 catacaccaa tagcaccgct tgggatactt caaaacggct tggatatttt tgcaacaaaa
      181 aaagccagcg tggtcttcta tccacgctga cctttttatg cgagccatga ggcccgtcgc
      241 cgaatggcgg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccctatgccc
      481 ttgcgcggct ccgccgcgca gtccggggtg cagggcttta gccctgctgc ggacggataa
      541 ggatgaaggg ggtcagttcg tcgccctgac cgctgggatt gtcggccatg gcgtcctgaa
      601 tctgctcgtc ggtcaggggc acggtgctgg acttgcccag ctgatcccgc tgcaggtcgt
      661 tggcgtccat caaaacgacc gggatgcccg tttcgcggct cagttcttca caaagctcgt
      721 tggggtgctc ggggttgatg agtgccatct cgtggtagag atcaaagctg gagcgctcgt
      781 agaagccgtc gatgccgccc actcccttgc cgcagacctt gtagaacacg ccgtgaacgc
      841 cgaaaagctt ggtcacagcg ctgcagaccg ccgccagcag cacccggggc aggcccacca
      901 tgtcgatgac cagctgcagc ttgtagggct cgtgcattcc cacgccggtg tggttgatgc
      961 ccgcaaagcg ccacaggaag ttggcccaga agccgggctt cacctgatcg cgggtcttga
     1021 cggacttgac gcacatgcac atgaccttgg cgccgaagct gagaatgtcg ccctcctgat
     1081 acagaggccg cacgtatttt tccaccagat ccttctggct ttcgcccacc tgcacaaagt
     1141 gggtctggat ggcgtagcgc tggtatttgc gcccgtcctt gccctcgatc acgccgcggt
     1201 caaaataaac gatgccgtcc ttctcccggc gctgctcctc cagattgcgg gaaggaatga
     1261 tccccataaa tcttcgcctc cgtacttttg ctgaatgttt ggataaggta gctttcccga
     1321 tttcggcctt cctgttgcac gaaaatcaca tgttttcgaa gaaaggttcg ccatcttcct
     1381 tgcacaggat acccatctcg tcaaaatgca gcggaatggg cttggccatg atgtacttga
     1441 gaatctcgtc aaagcggacg gattcggtgt aggtcagcag ggtgaacgcc acgccgtgcc
     1501 tcttggcgcg gcccgtgcgg ccgatgcggt gcaggtaata ctcgttttcg ttgggcaggt
     1561 cgtaattgat gacggcctcc acatcgtcca cgtcgatgcc gcgggccgcc acgtcggtgg
     1621 ccagcagaat ctggaacttg cccttgcgga agccctgcat cacctcgttg cgcacgctct
     1681 ggcgcatatc gccgtgcagg cactccgccg gatagcccgc gtccttcagc cgcttgcaca
     1741 ggcgctgggt catgtccttg gtgttgatga agaccatgat cttctggtaa acgtcgctgt
     1801 ccagaagata gagcaggttt tcatacttct gctcccgctc ggtgttaatg acgtactggg
     1861 tgatctgagg ccggttttcc ttggtggcct cgatgcgcac ctccaccggg tcgttctgat
     1921 acttccacgc gatggtcaac acgtcctgat tggtggtggc ggagaacagc accagctccc
     1981 gctcgatggg cgtggcttcg atgatccggg tcacgtcctt gacaaagccc atgttgagca
     2041 tttcgtccgc ctcgtccagc accatggtgt ggacggcgtc cagacggata ttgccccggt
     2101 tcatgtgatc cagcagtcgg ccgggagtag ccaccacgat ctggggattc tgctgcagct
     2161 gcttgagctg cttgacgata ggctgaccgc cgtacaggca ggcgatgcgc acgccgggaa
     2221 tgaactgggc cagcgcccgc agctcgtcgg tgatctgcag gctcagctcc cgggtggggg
     2281 ccagcaccag ctcctgcacc cggcggtcgt tcaggttcac gtattccagc atggggatgc
     2341 cgaatgcgca agttttgccc gtgcccgtgg gggcgatggc gatcacgtcc cggccctcca
     2401 tcatcagggg cagggtctgc tgctgcaccg gcgtcatttc ctgaaagccc atcttggcga
     2461 cggctttgag gatctccggg ctcaactccg tcgagtcgga aaatttcatt ggttctgtgg
     2521 tttccaattt ctgctccttc tgcgttcagg tcatgagcct gttattccgc gtgttgctga
     2581 tagtactgct cgatggcgcg gctcagctga tccgggcagg aggtattgcc ccggcactga
     2641 atgcccctca gacggttcat cacgtccttg gcgttctggc cctccaccat tttggccagg
     2701 ccctgagtgt tgccccggca gccgttggta aactggcagt gggtgataac gtcgttttcg
     2761 atctctaaat caatcgccgt agagcaggtt ccgcgagtct gataacgcaa gtaacgtcct
     2821 tcctttcctg cggcgtactc cgccgctttc ctcacgtctg tgacatatcc ataaatacgc
     2881 cacttaacgc tattcgacgt cttttccgtt tttcctgcca aaattcgcac gccctgcccg
     2941 gattcccata ggatgaaatg attctatcgc cgcgaggcgc aagggagcgt tgtcatgaaa
     3001 attctgatca cggactccga gcgcggtgcg tttctctgga aggatgggga agccctgcgc
     3061 ccgctgccct gtcccgccga atgcccctac tgcccggcgg ccagccggga gcggctgttc
     3121 ctctgctgcc gaaaaaaccg ggactgctgg tgcctcagcc gacgcacgct gcagacggaa
     3181 cgggtctttc ccgcgccgcc cgcgctggcg gcggcgtgtc cctcgccttg cggaaaatac
     3241 ctgtatctgc tcagcaccga agcagacgcg gtgcagacgg tctcgctgga aacgggcgaa
     3301 ctttgctatg ccgccccggc gggggtcttt ccccgttcca tgaagctgca cccttccggg
     3361 cggttgcttc tgtgtgccgg gggcgcggtg aacgaagcct gcctgctgaa tgcgcccggc
     3421 ctgaccctcc gccatgtctt ttatccgaaa agtccctgct tcggggcgga tttctggcgg
     3481 gagggcgtgg tgcttctgtg cgccgtggag ggagaaaatt tgcagacggc cgtgtgtctg
     3541 ggccggcccg ggcggccccg cacccgggag atccagcgtc tgccggggca gcccggagcg
     3601 ctgtgcgtct gcccggacgg gggcagcgcc ctgatcagca ccccggacgg gctgatgaag
     3661 ctgtcccttc ccggcggcga gctggaatgg aatctgccgg agtgggccct atgcatgggg
     3721 ctctgctgtc agggagggat ggcgctggtc tcggacgcgc tctgcggccg ggtctgcctg
     3781 ctgaacctgc gccgcccatg gaaaaaccgc attctctttc agggcacaga cgctcaggcc
     3841 tgttttctgc ccgaggagca taattcctcc ccttccgggg caaactcctg aaaaagcccc
     3901 cgggcttgca agaaggaggg aaccttatgg taaacacgat cgcattggtg ctggtcatta
     3961 tcggggccat caactgggcg ctgatcggcg tatttcagtt cgatctggtg gcgtggatct
     4021 tcggcagtca ggcggcggtc tggagccgca tcgtctatac gctggtaggc gcggcagggc
     4081 tgtggtgcat ctccctgctc ttccccgagc attccgccgt caaggagtaa gcgaagctgg
     4141 gaggcggcct ccatcagaaa ggcccggttg gtgggcttgc ccttgtccgg gtcgatggtc
     4201 gcgccaaaga aacgctcgat ttcgggaatg ctgcccatgg tgaacacgcc ctccacggcc
     4261 accctgaggc agcgctccac cgccccggcg ctgacgccga aggcgcgggc gcactccgcg
     4321 tacagccggt tgacgggctg ctgatccagt ccggggacgg ggacccggcg gcacaggcac
     4381 cacgccgcat agcgccgtcc cttccattcc cggcgcattc ccagcgcgtc cagaaaacgc
     4441 tctgccgccc gctgtgtccg ggcacggtga gccgctgcca gacagggcag cggctttttg
     4501 gcgagcacgg aaagcagcgc agccagcctt gcgccgctgc agtccgcagg cacggcgcca
     4561 tccgccgccg ggcgcggacc gtcctgccag agggccagca ccctcggcgg gcacaacggc
     4621 ggcgcggcgc acagcttttc ccacaaagac gttccgtcag cccgcaggcg gctgtccagc
     4681 agcagcacat cccagtgttc catttccagc agggccgcgg cctgaagccc gttctccgct
     4741 acgcgcacgt cccagtgctc gtccatcccg tacagccgaa gccgtacctc cagcgctttc
     4801 ttttcctcca aaatggcgat cagcagccgc aagacggttt cccccttttg ttttttcgac
     4861 cgaaagatca ttcaaaagga ggttccgtcc cgcggctgcg ctttcctgcc tcaaaatttc
     4921 gctttgtttg tcgaagaccg ggttattttc ccaaaaattc cgccgcctga ttgaccgcgt
     4981 ccaccacggc ctgtgaagaa accgtcgcgc cggaaatacc gtcgatatcc ttgcccagct
     5041 ccaagggcgg cgttttgccc acaaactggc ggatgaagct ctcgtctttc accttgccgc
     5101 ctacgccgtc cgtctcggca aaccgggcct cgcccacgtt cagcgattcg atggctccgt
     5161 cggcgttcag ccgtacccga tgacgctggc gttggcttcc cggacaggct gttccgtttc
     5221 cgccgcgccg cctgtttcgc cgccggtcag agactgcacc ttccccacgc cctgattcac
     5281 gccgtccacc acggcccggc tggtcacggt cgccccggaa atgccgtcga tattttcgcc
     5341 cagctttagc gggggcgttt ttccggcgaa ctgatcggta aactccggct ccttcgcccg
     5401 ggcccccagc ccttccgttt ccttgaagtc agggccgccc acgctgatcc cccgcagtgt
     5461 cccgccggtc tccatgccgg tcagtacctc gatggagccg ccgtacccct gtacggtctc
     5521 cttgaccgcg tagccaatgg tctggccgcc ctgcttcacc tcgtatacga agtccacgcc
     5581 gctgctctcg ccggggctca gctccgcaaa ctcgtccgcc tcgggaaaca tggccttcag
     5641 cgcctgctga ttttcgccca gattttgttc cgcaatgggc ttttccgtca tgaggttggt
     5701 agtggccagc gccaccgccg ctaccagcgc aatgaccgtc agcgccagcc atcccgccag
     5761 ctttttcttt ccgtccatac ccgcacgctc ccttcttttc cgtcttatgc tgtgaagccg
     5821 cgccgggttc tatgcgtcca aaacctgtcg aaggccgtcg cacggctgcc atttttcgcg
     5881 ccgggaatcg acaggccgca tttccggccc tgtctatact cgtttcagca tcattcgtca
     5941 tcaaagcgca caggaggtgc tttttccatg caaagcgaaa tgcagaccga agctggaaga
     6001 agaaatcccg cttccgtcaa agcgaaattt tcggctcgtt ctttttttca atgggcatat
     6061 gccacgctgg cctgttcggc ccggcagctg gggctttgcg cctgtttttt cctgctgccc
     6121 ttcgtgcgct ggttcggcat tccttcgccc tttgcggcgg cgctgcttct ggggctgaac
     6181 cgtccgccag aagtgttttc cctgctgggc atgacgctgt cgctggctct gcggctgctt
     6241 tggggcgtgg aaagcgaccc ttggcaatat gcggggctgt tctttctatg gttgttgctg
     6301 cggcgctccc gtccccggcc cggcattgaa acggcagcgc tgggcggcgc ggccatgatg
     6361 cctcgggcga tccagagcct ctttaccggg gaaccgctga ccatgctttt atcgctggcg
     6421 gcggtgccgg tgtgcatggc gatttccgca tggttccggg atgggctgga tcgaacgggc
     6481 tttgcgccgc cccgttttcg ggacaaggcg gtgcgaatct ttctggggct tctgctgatc
     6541 tccgggctgg gctattttca gcttttcggc gtttcgctgg gacaggccgg ggcggtgctg
     6601 ggcacgctgc tggctgccgg gcaggcaggc tgcgccgccg gggcggctgg tgggctgttc
     6661 tgcggattgg cgctggcttt cggcggtcac gactgccgca ttgctttcag tctgtctctc
     6721 tgcggacttc tgtgcggcgt tccctttctg aaaaagaaac gaccgctggg catggcggcg
     6781 gcgtttgcgg gcaatctgct ttcgtttttc gtaaccccgc tgacccagcc gaccctgggc
     6841 tggctggcgg tggcgctggg cgggctttgc tacgtgctat tgccaaaatc ccgtctggac
     6901 gaagtccggg agctgctctg tcccctgggc cgtcagtccg gcggcatgga cggagccttt
     6961 cttcgggaga ccatcgggcg gatgcaggaa tccatccgca gcgtcgccaa ggcgctgccg
     7021 caggtagcgg agacggttcc atcggagggg ttggagctgg gggaactgct ctgcgcccag
     7081 tgttccaacc gggagctttg ctggggacgt gcccgtgccc gcacggaaaa gctgatgagt
     7141 tccatgatgg aacgaagccg tcagggcgaa cccatcgagg aggacgcgct gcccgcctta
     7201 agtcaacagg gctgtttacg ggcggaatcc atcccggagg cggcgcagga ggcgctgtcc
     7261 ctgcaccagc agcggctgaa ccggcagaag cgcgcccgtt acgagcggga gctgaccctg
     7321 acccatctgg cggccatgct gggttcgctc agcgagctga acacggtcgc ggcgggggag
     7381 agtctgaacg acttacaggc ggcgcacgtc atccgtcagg ccatggacga gctgcacgtg
     7441 cccgcccagc ttctctacgc ccgccgagtg gacgggcacc tgcaggcggc gctgcaggca
     7501 gaagccatgt ttcccattca gaagccgctg gaggcgcttc ttcgtnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaagg gacacatcga
     7621 gctggaaaaa ctgccgctat acagcgcctc catcggcacg gcatcggtct gcgccgggca
     7681 gcgggactcc ggcgaagggg aaggcatttg cggcgacgcc agcgcggccc ggcgctgcga
     7741 cggcgggcgg ctgctgctca tgctctgcga cggtatgggg cacggcaagg aagcccatga
     7801 cttaagcgaa aagaccctcg aactgcttct gcttttgatg gaagcgggct acacccggcg
     7861 gcaggccatc acggcggtga acggcatcat gctgtcccag aaggagcagc cggagggatt
     7921 ttccaccgtg gacttagcgg acgtagatct ctggacgggc gacgttgcgc tggaaaagct
     7981 gggggcctgc gccagctggc tgatccgcgg cgatcacgtc aaaaagctgg aagcgtcctc
     8041 actgcccctt ggggtcatgg aagaagcgcg ctccacctcg gtagagtgcc gtctgcacag
     8101 cggggacatt ctggtgatgc tctccgacgg catctcggac gttttcccgg acgaagcgca
     8161 gatgcgcaaa atgctggagg aaagcgtcta cattcagccc cagcgcatgg cggacgcgct
     8221 catccgcaat gccattctgg cctccggcgg cacaccccgg gacgacatga ccgcccaagt
     8281 tctcctcctc atgacccggc agcaggggga gtacccctgc accccggact gcttgcaggg
     8341 gcatagaagt gatgcggctt gaatcggttt tnnnnnnnnn ngtgccacgg ctctcgggtt
     8401 tggtcgctcg aaatggggcg ggtgttttat ggaaggcgga tgggcgacgt ggtgtgcgga
     8461 tcatacaagt acggcgcata cagaaagcgc gaagaatacg gtccgccctg agttttatga
     8521 tttcctgttt caaagggact tcattgtttt gtccacagtt tcgtcttcct tggcattagg
     8581 aggaagacgg aactttctta ttattcccaa aagagctgct atttgccgcc tttccctcaa
     8641 aacccatatt tgaccgatcc tatatcctgt gctataataa actgttcata aatacattcg
     8701 ccgcccggcg gcgcaattac cttaacaaag aaaggctgat acccatgtcc ggacacagca
     8761 aatggcataa tatcgcagta aagaagggca agaccgatgc ggcccgcggc aaaatcttca
     8821 ccaagatcgg ccgtgaaatc gctatcgccg tgcgcgaagg cggcagcaat cccgaaagca
     8881 actccaagct gcgcgacgtt atcgccaaca tgcccaacga caacatcaag cgttccatcc
     8941 agaaggcttc cggcgagctg ggcaatgtgg attatgaaag catcaactac gagggctacg
     9001 cccccggcgg cgtggcggtc atcgtggaga ccatcaccga caaccgcaac cgtaccgcca
     9061 gcgatgtgcg tcactgcttc gccaaatacg acggcacgct gggcgtgacc ggcagcgtgg
     9121 gtttcatgtt cgacaagaag ggcgtgctgg tagtggagcg caccgccgac accgacgagg
     9181 acgaaatgat gatgaccgtt ctggacgccg gtgcggaaga catgaagacg ctggatgacg
     9241 ccttcgaaat ttacaccgac cccaacgagt tctccgctgt gcgtgagcag ctggaaaacg
     9301 gcggcatgac cttcctgagc gccgaagtgc agatggtgcc ccagaacacc gttgctctgc
     9361 ccgacgaaga gaccctcacc aaggtacaga agctgctgga catgctggaa gacaacgacg
     9421 acgtgcagaa cgtctggcac aacgccgagc tccccgaaga agacgacgag gactaagcag
     9481 gggcagtgcc cctgcacccc ggactgcg
//
LOCUS       DAWAEA010000010         9052 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_1248_length_9052_cov_2.348783, whole genome shotgun sequence.
ACCESSION   DAWAEA010000010 DAWAEA010000000
VERSION     DAWAEA010000010.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 9052)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9052)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9052
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1248_length_9052_cov_2.348783"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3629..3638
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4466..4475
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4712..4733
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8539..8548
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1832 a         2746 c         2676 g         1746 t
ORIGIN      
        1 tggagctgaa ggaaggcggc tggctgcaca gctacgacac ccccgtcaag gaaagcctgc
       61 gcttccccta tgacgaggaa ggctactggt accctgtgcg cgtgtgcaac atggtgctgg
      121 cctacaatcc cgagctcaag acccccgaag agctgcccaa gaccttcaag gagtttgccg
      181 aggatcccag cctgaagggc cgtatctcca tgggcaaccc tctgacctcc ggcaccacca
      241 tggcggccgc ggccagcctg agcgacaagt acggctatga gtacttcacc gctctgggca
      301 acaacgacgt gatgatcgaa tccggctcca ccgctctggc caagctgcag accggcgagt
      361 gcgacgtcat catgattctg gaagaatccg tgctgaagga gcgcaaggaa aacggctcca
      421 agatcgcctg catctatccc gacgacggcg ttatcctgat cccctccacc gttatgaccg
      481 tggcggaaga ccgcagcgcc aacatgaaca tcgaggcctg cgaagccatc accgattacc
      541 tgctgagcga agaaggccag aagttcatgg tggagggcta catgcactcc gtgctgaagg
      601 gcttctccga ggttcccttc gattccgtgg acaccgacgg cctgatcgag aaggacatgg
      661 gcgttgactg ggtgcgctgc tacaccatgc gcgacgagat ccgcaccaag ttccaggagg
      721 ccgttaccat tcccgaaaag aagtaaatcc ggttaacgca aatcgcaaag ggtgaataag
      781 gcgccgccgc tgcagccgac gaacgatgcg gctccgcctg atcctaccga catgccaaat
      841 gggggaagtt gtcctgacgg cggcttcccc ctatcgtata acgaccaatc aacatactgg
      901 ggagtgctgt tcatgcagac acagcttcaa cgaagcaaac atgatccatc cgaaacccgg
      961 tgtaagctga cgctgctgat cctgtttttg ctgggcgccg cgctgatttt gggcggcttc
     1021 tggggaatcc ggcagtatca gcagtctggc ttcgatcagg aagtaggcta ccggtatatc
     1081 caaaagctgt ccggcgaagt ggaaagcctg tatacccagt ccatccagcc ggtcatggcc
     1141 aggctgacgg acgcggagcg ggagtccctg accctcgagg ccgccgccct gctgaaggaa
     1201 aactggacgg ctgccgtcaa ggccgaccag agcgcccgca cggacgaaat gctggccggg
     1261 ctggaaggcg ataagctcca gcagaaaacg atggagctgc tttccatcac ctattccctg
     1321 aacgggccca aggtggcatc cgccgaaaag aagctgctgg cccaatggac ggacgcggag
     1381 cgtgaccaga accgggaagc cctgcttcgc gcgctgctgg acgaaaacgc gcccgtgccg
     1441 caggcggccg cttccgccgt gggcgaactc accggcagcg aacgggccgc cgcgctgatg
     1501 cagctgtttc tggtcagcgc caacgctcag aacgagcccc tgcctaccga ccatgtgaac
     1561 ggcctgaaaa aagcggcccg caacgccgag gcccgcattt ccgcctacga aatgacggcg
     1621 gacggttccg tgaccctgct ttcccaagtg ctgatctccc acagccgcac ggccattctg
     1681 ctgggcattt tgctggcgct ggacatcgcg cttctgttcc tgatgctgta taaggatcac
     1741 agctggcatc tggatttcaa gtgggccgtc atcctcatca ttgtggactt catgctggtt
     1801 ttccaggtgc tgcccatggt gtatctgatc atcaaggcct tcttcccgga gggcagcttt
     1861 acctttgagg tattccagcg gctgtacacc taccagatga atctgggcgc gctgaagaac
     1921 accctcatcg ccgccaccgt caccatggtg ctgggcacgc tgatcgcctt cccgctggcg
     1981 tggctggtgg gccgcaccaa tctgctgggc aaaaagttct tccgctccct gttcgtgctg
     2041 acgtacatgg tgccccccta cgtgggcgct atggcgtggc tgcggctgct gaaccccaac
     2101 gtgggcacca tgaatcagtt cttccgcatg attttcggtc tgggcgatac gcccggcccg
     2161 ctgaacatct acaccttggg cggactgatt tgggtgctga ccacgttcta ttatccctat
     2221 gccttcatca ccatctcccg ggccatggag aaaatggacc ccagtctgga ggaggccagc
     2281 cgcatttccg gcgcatctcc cctgctgacg gtgttccgca tcactctgcc catgatgacc
     2341 cccagcctga tcgcgggcgc gctgctggtc tttgtggcag ccatgagctg ctacggcatc
     2401 ccctccatta tcggcgcacc gggtaaggtg cataccgtca ccacccgcat catcgagtac
     2461 aacggtctgg gcgcacaggg catcagcgat gcgaccggcc ttgcggtctt cctgatggcg
     2521 ctggcgattc tcattctcta cctgagcgac ttcgtagtgg cccggaagca gtacatcacc
     2581 gtatccggca aatccacccg gcccaacatc gtggatctgg gcaagtggcg cgtcccgctg
     2641 acggtgctgg tgagcctgtt cgccaccatc gtggtgctga ttccctttgc caccatcctg
     2701 accacctcct ttaagatcga cgtgggcaag tccctcttcg actcggagaa cttcaccctg
     2761 acccagtggc agaccatctt ctcccgctcg gagaccatga gctgcctgaa gaacagcctg
     2821 atcttcggcg tggtcaccgc aacggtgggc atcgtggtgg cctgcaccat gtcctacctg
     2881 ctgcagcgga cgaagattcg cggccgcaag ctgccggact tcctcatcac cctcggctcg
     2941 ggcactccct ccgtggtcat cgcgctgggc cttatcatga ccatgaaggg cgacttcggc
     3001 gtgaacatct acaacacggc ctacatcatg atcgtcgctt atctcatcaa atacctgatg
     3061 atgggtatgc gtaccgtggt cagcgccatg agtcagattc acgtctcgct ggaagagtgc
     3121 agccagatct ccggcgcaag ctggacgcgc accatgctta agattaccgg ccccctgatc
     3181 ttcccctcca tcgccgcggg ctggttcctg atcttcattc ccagcttcta cgagctgagc
     3241 atgacgacgc tgctctactc caactccacc aagaccatcg gcttccagct gtacgagtac
     3301 tggaccttta ccagccagcc tcagagctgc gccatggcct tcggcatcct gatgatcgtt
     3361 gtcgtcctga acttcctgct caacaagctc accaagggcg aattctcgat ctgatttgcg
     3421 tcagccgttc catcccatct taaaacaccc cttccgattg atatgacccc tcttgccgct
     3481 ggtcgacaag gctcgaacca agtgcaaagg gatttcccac aaaaaggagt tggaatcatg
     3541 tccagcgtaa ccattcaaaa cgtgaccaag accttcggca acaccacggt gctggaaaac
     3601 tttgacgccg tgtttgcgga cggcgaatnn nnnnnnnngt gttcgacaac gtggcctacc
     3661 cgctgcagat caagaaggtg gacaaagccg ccattaagca gaaggtggag cgcgtgctgg
     3721 acgtggtgca tctggcccag taccgggatc ggatccccag ccagctgtcc ggcggacagc
     3781 agcagcgcgt cgcgctggcc cgggcgctgg tggccgagcc cgccctgctc cttctggacg
     3841 agcccctgtc caatctggac gcaaagctgc gcgagagcat gcgctttgag atcaaggaca
     3901 ttcagaagca gtacggcatc accgtggtgt acgtcaccca cgaccagacc gaggccatgg
     3961 ccatgagcga ccgcatcgtg gtcatcaacc ggggcgttat ccagcaggtg ggctccccca
     4021 aggtgatcta caatgagccc gccaatccct tcattgcgga ctttgtgggc aagatcgagt
     4081 tcctggaggg ccatgtggag gccggctgca tcgccctcaa cggctgcggc cagaagctgc
     4141 cctacgaagg cgacgccaag ggcgaggtca tcgtaggcat tcggcccgag catgtgaagt
     4201 acgccgaggc aggcaagggc acgctgcacg gcgtggtgaa gagccacttc tatttgggcg
     4261 acgtagacga ctgccgcatc gatctgggcg gcggcaagga gctgcgcatc atcgccgacc
     4321 cctacgagag cgtgggcgtg gaagcgggca aggccatcga gctggacgtg cgcaacttcc
     4381 tcgtctatcc cgccgagcag cccggcgaag acttccgccg catcctcacc taaggcaggg
     4441 gcagtgcccc tgcaccccgg actgcnnnnn nnnnntttct accgccgctc ggcaacaggc
     4501 ctcgcggctc gcggggctgg tgtatgaatc gccagttggc tgaaaaaggc cgccgttggg
     4561 cgacaggaag gggcaatgcc cctgcacccc acatcgcgcc ctatgggcgc ggggctgggc
     4621 gagggatggg gcatggccgg ggttttaccg ccgctcggca acgggcctcg cggctcgccg
     4681 gtctggtatg atttcctgtt agttgaatag annnnnnnnn nnnnnnnnnn nnnggcctca
     4741 tggctggcag cgtgtccaaa acacccttat ttttcataaa atcaaagcag ggaatgtttt
     4801 ctgaagtgct agaaaacatt ccctgcttta tatttttaac gtttttttga atgaatcaaa
     4861 aagagacatt cctctgacat gcagggtcat aatgccccac tccagaggaa tgtctctata
     4921 aacagtatat ccaatctgat taagccgtca atgaaaaatt aaaaaatttt ccatcaggct
     4981 gcacgttttg gagtccatta cattcataac acacagggct ggatggcagg tttattccca
     5041 ccagaccggc gagccgtggc acccgtcgtg ccacggcggt aaaatcccgg ccaatccgca
     5101 tgtcccccga cgcccctgcg cggcaaaacc gcgcagagat gggtgcaggg catcagccct
     5161 gctgctgaaa gtcggcaagg agggaggcga aaacgtccag agccgcttcg atgggagcgg
     5221 gcgtggacat gtcgatgccc accgtcttca gctcttcgat ggggctcatg ctgccgccca
     5281 aagtcaggaa acggcggtag gccgctacat cctcgggcgt gccgtgcagg atcttgtccg
     5341 ccagagccac ggccgcgcag aagctggtgg cgtatttgta gacgtagaag gaacggtaga
     5401 agtgcggaat gcgcatccat tcgttctcca cgaccttgtc cacccggcag ccgccgccgt
     5461 agtactgctt gttcaggtcg tagtacatgg cgttcaggga ttcccgggtc aggggctggc
     5521 ccgcttcttc catggcgtgg gccttcatct caaattccgc aaacatggtc tggcggaaaa
     5581 cggtggtgcg gaagccttcc agcagacggc cgatcagggc ctgctgcgcc gcctgattgc
     5641 ccgcaaactt cttgcgcagg gcgttgatgg tcagcacttc gttgcaggtg gaggccactt
     5701 ccgctacaaa gatggcgtag tcgctcttga tgaagggctg ggtgtggttg gaataatagc
     5761 tgtgcatggc gtgacccatt tcgtgggcca gcgtcagcag gccgtccagc tcgggccgga
     5821 agttcagcag cacgtaggga tgcacgccgt aggcgctgcc gttggagtat gcgccggtgg
     5881 tcttgccctt gttttcgtaa acgtcgatcc agccgccgtc cttggcggaa cgcaccacgt
     5941 cctgatagtc cttgcccagc ggggcgacgg cttccagcag cagctcatag gcctcgtcaa
     6001 aggtcagctt catctggaaa tccttggtca tgggcgcgta caggtcgtac atatggacgg
     6061 tggggatgcc gaaggcctcc ttgcgcatgc cgcagtaggc gtgcagcacg ggcagcgcct
     6121 cgtgaacggc cttgagcagg ttgtcgtaga cgctgacggg gatgtcgtcc gggtacaatg
     6181 ccgcttcccg ggcgctctgg tagccgtggg ccttggccag gaaaacgtcg ctctttacat
     6241 tacccgcgta ggtggcggcg aaggtggagc cataagcgcc gtaggccttc atcatgcgct
     6301 cataggcggc cttgcgcaca gagcggtcag ggctttccag catagagcca taacgggcat
     6361 gggtcagctc gaccatcttt ccgtcctcac ccttgacctt gccaagactc atgtccacgt
     6421 cggtgagcat gtcgtataca ttgcccgtcg cgccgaaaac ctcctgaccg ctggccagca
     6481 gggtctccac ctcggaggac agggtgtggg ggcgcatccg ctccagctcc ttcagcatca
     6541 cgtcgaaatc cgcaaaatcc gggtcggcga tgtagccttt cagggtagcc ttgggcagct
     6601 tcaaaagctc gggggagata aaggccacgg cggcgctgta ctgggcgtac agggactggg
     6661 tgcgggccat cacgccctga tagaaggaat ctccgttatc cacgttcagg ttacagctgg
     6721 cataggaata caggcggctc aggtgctcgt tggcctccgc ataggttttc agcagagaaa
     6781 gcacagtatc cttgccgttc ttcaacgttc cggcatagga ggccagctcc cgggcctttt
     6841 cctgaatcca ctggaaatcc ttctcccatt cttcctgaga ggcgtaaatg tggctggtct
     6901 gccactgggc ctgcgcggga acgtccttgc gcagcaaaac ttcgctcatg atgaaatcct
     6961 ccttgccgcc gtcaggcgga aattgttcaa aagtattgta gaccaaagga agggaaagaa
     7021 tcaaggggaa aaggcaaaaa cggacaaatc acagccgata gcagccgtag ggcacgtttt
     7081 ccctgcggcg ggcgcagcgg ctttccttca tgcattgaat agagcctgcc agctcccggc
     7141 gctcaaacag ctccgtcacc cccagccggg ccagctcccc ggcaaaggcg tcgatgcgct
     7201 cgcttctgcc gggcgcgtcc atgggcaggc gggccagccg cacgtatgca aagctcaggc
     7261 gctcccgctc cagccgctcc cggcgcaggg gatcggcggt cagcgacagc gcgtccgcca
     7321 gacagccgtc ggcctgctcg atgagccctt cgtccagata agccgcatcg ggcatgtcat
     7381 aaatgcccat gtgggtctgg gaggcggcgt tttccatcag agacaggcat tgcagcacgc
     7441 cgggctccgc ctccgggccg tagtagcccc gcacaaagga gcggatcaga ccgtccgtgt
     7501 ccgcgtccgc gttccagagc agcctgctca gcagataaat gcgcagcggc gcaaacgcac
     7561 tggtctttcc gggggaatag ttgccctgct ccagcacgcc ctctacgccc aaacggcgga
     7621 acagacgcag gttttccccg atcacatgcc agttggggaa gggctgcaaa taattgctga
     7681 aattggtggt gtaatcccac aggtacagat ggggcgcgat tttggcccag tcggccagat
     7741 cgtcggcgaa ggcctgtccg gacaaggcaa acccgtcggc ggcgttgttt tccacgtcga
     7801 tgccgtcgat ggcctgacgg cacacggcca tgggatggct ctgacagcac tcgatgctgc
     7861 acagccggac gatcacgttt tcccggggcc ggagataccg gggcgctttc cgggtataca
     7921 gataggcgaa ggtgtgaatg cggttttccg ggaattccgc ccggacagcg tccgccacgg
     7981 cgttcacgaa ggccagcatg gttccggcgt ggctttcctc ccgccggtcg atggcctgac
     8041 aatgctcgca ttcgcagtag ccgtaccagt cgttctgagc cacggagaaa atgcggcact
     8101 ccgggtgctc ccgcatccag cgtcgcactc cttcaatgca gatttccagc acgtccgggt
     8161 ttgtcaggca gagctggcta tgctcccgcc ggcgtttccc gttcaccagc gagaaatatt
     8221 ccgggtggga atcaaaccat ttttccgggg gaaccagctc ctcaaaggta tgggaatagc
     8281 gatagaagct gattttgccg cccatttccg gcgaaatctc cgcccgggcg gagttcagtc
     8341 cgcactgcag ggcgaaccgc ccgttcagcg caccccgcca gtacagatcc cgataggcga
     8401 aatcgggccg ggaggaaaag ctcatgggca tccactccgt gacggaaggc agaatttcac
     8461 agtcctcggc cagaaactgc tcccccagcc gctccagcag ggcgtccagg ccgggctcct
     8521 gcccggccag caggcagann nnnnnnnncg tatacgccgt agaccgccgc ccgggggctt
     8581 tcgccctgaa tatcggtatg acgtccagtc acggacacgc ggtagccctc gccccggccg
     8641 tgcccggcgg tcacggtgaa gggatacccc tccgggcgaa gctccgcgcc ggtcatggcg
     8701 gtcaggcagc ggctgagctc ggattgggcg aagggcatat tgccggtgat aaagacggac
     8761 tggggaaagc gcatggaaaa actccttttt ttggctcaat cggtgataga aacgggcgga
     8821 tggatggcgg tttcagtata gcagaaaggt ggagagaagg gaagagggaa agggggaaaa
     8881 gccctttggt gcgcccgcag gcgcaggcct tctgccggtg gggactgacg cgccgactac
     8941 ggcgcggaag tgggacttgc agtcccaagg ggcgttcata cttttctttc gatggcgaaa
     9001 gaaaagtatg caaaagaaag ccagcggcac ggcgactccg ggaaaaaggc tc
//
LOCUS       DAWAEA010000011         8919 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_1275_length_8919_cov_2.500000, whole genome shotgun sequence.
ACCESSION   DAWAEA010000011 DAWAEA010000000
VERSION     DAWAEA010000011.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 8919)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8919)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8919
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1275_length_8919_cov_2.500000"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6790..6799
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7713..7722
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1540 a         2854 c         2762 g         1743 t
ORIGIN      
        1 cccctgcgcg cctatggcgc gcagtccggg gtgcagtggc accgcccctg ctagtcgtct
       61 tgggatttgg tttcggcgag gaacttttcc agcttgcgct ttacgcgctg aagggcgttg
      121 tcgatggact tcacgtggcg gcccagtttg tcggcgatct cctgatagct cttgccgtcc
      181 agataggcgt tgagaacgtc ctgctccaga ctggaaagca cctgatccac ccggtcgcgg
      241 atggagacca gatcctcctg cccgatgatg agatcctccg ggttctgagc acggccttcc
      301 acgatcacat ccatgagggt gcggtcgctt tcctcgtcgt aaatgggctt gttgagggaa
      361 acgtagctgt tcaggggcac gtgcttctgc cgggtggccg tcttgatggc ggtgatgatc
      421 tgccgagtga tacatagctc tgcaaaggcg cggaagctgg acaggcgctc cggctgatag
      481 tcccggatgg ccttgtagag accgatcatg ccctcctgca cgatgtcctc gtgatcagcg
      541 ccgatgagaa aatagctgcg cgccttagaa cgcacgaagt ttttgtactt gtacagcagg
      601 tattccaccg cggcgtcgtc atgctgctgg gcctgtgaca cgatctcctc gtcggtcagc
      661 ccggcaaagc gggtggagta ttcccatgga ttttcctcgt ccggctcatc gaagtcctcg
      721 gacgctttct cgtccccttc gttgggatcg gtttcttccc cttcggaaag gatgggttcc
      781 agttcttcgt cgccgagttc gttcaggctg tccatttctt cctgttcgtc ccgctcgtcc
      841 gtcacggcag ttcacctcct gttcccgttt tccgcatgaa tgcggaaaat aaaatcagtc
      901 ttttcgcctg cggatctcgt ccagaagctc gtattgcgcc cggggcatgg cagcggcaat
      961 ggtggacgcg ccgctggccg gatcggggcg gtgctgatcc cgaccccggc tgcgggtctg
     1021 gttcagctcc caccatagct cccgggagct gatgcgggta gcgccccggc ccagaatgac
     1081 cgtctgctcc acattgtcgc tggtagccac ccgtgcctcc cggtacttgg gcatttcctg
     1141 gcactgccgt tcgatgtagt gatcggcggt ctctccatgc cgggtgtaga ccacggtcag
     1201 gccgtcccgc tcgtccactg tgcgcagggt gcggtcggtg cggtagccgt cgaacaccag
     1261 ccagacctcc tgaccgctgt acccggcgta atcctccaat aggtggatca gccggtcgcg
     1321 gcacacgtcc agaggccagt tctcccgctg cgcctgcggc catgcgccga tgacgttgta
     1381 gccgtccaca tataagagcg gtttcatggc ggaattaccg ggaacgcatg accgcgtaca
     1441 tcagcacgcc cgccgccacg ctggcgttca ggctgtccag atgaccccgc aggggcaggg
     1501 aaacggtctt gtcgcagcat tccagcaccc ggcggctgac gccctcgcct tccgcgccga
     1561 tgaccagcgc caccgcgccg tcaaacttca cgctggcata gtcctcgccc tccatatcgg
     1621 cggcgtagac ccaaatgttg cgctccttca gagcttcgat ggtgttgccc agattggtca
     1681 cgcgggccac gggcaggtat tccaccgcgc ccgcgctggc cttcaccgcg gcgggggtca
     1741 ggcccacggc ccggcgctcc tgcacgatga cgccgtgcgc gccggcgcat tccgccgtcc
     1801 gaatgatcgc gcccaggttg tggggatcgg tgataccgtc cagaagcacc aggaagggct
     1861 gctcgttccg ggcttcggcc agctccagca tatcctccag cgtgctgtaa tgataggcgg
     1921 acgcaaaggc cagcattccc tgatgattgt gggtaatggc gtccagacgg gcgcggtcca
     1981 cttcctgtac gggaatgtgg cgttccttgg ccatctgcac aatctcccgg gcggagccgc
     2041 tgagctcgcc ccgggccacc agaagctttt cgatgtcccg gccgctcttg agggcctccc
     2101 ggatgggatt gcggccgctg agcagattct cgttgggcac cacgtccggc tcggtctcca
     2161 cggcgggggc gggctgatag gtcacgggcg cggcatagcg gaacttgttg tagccgcttt
     2221 cctcgccgct gcggcgggcc ttgcgctgct cgtcgtagga ggcgggggtc acgttgctgc
     2281 cccgttcctc gtagcgaggt gcgtttttgg agaagcggta ttcgccctgc tggggcttgc
     2341 ggccttcgct gcgaccgtcg cgctggtcgc cgtcaaagcg gcgctcctgc ccgggacggg
     2401 cgctgccgta acgcttgccg gactccgagc ggtgatcgcc gccataaggc ttgcggtcgt
     2461 tattccgatg gtcgccgtag gacttgcgtt cgccgtcgaa ccggcgctcg tcggagcggg
     2521 aaccgtcgtg gtagcgcttg ccctctccgt tccggctgac gcctgctttg ccgaagctgt
     2581 atttgccggt gtagtggctg ccctcgtcgt caaagccgcc ccgattctgg gccacggcct
     2641 gtttttccct gcgggtcttt ccttttacgt ctttataaaa tgccatgttg tttcctgctt
     2701 tctttttggt tcgttccatt ttgtttcagg tgattcaggc ttctgctagg cctgacggct
     2761 gattttcccg gaggaaggct tcccgccggg cgcgcatttc cgcctgacgg ccgcagcttt
     2821 tggcgccttc ggggcagccc cggcggagac attccggccc cgcgtccgcg aacagggcgg
     2881 ggacttcctt gtagcagagg gcgaacatgc tctccgccag tgcgcgaatt tcccactggg
     2941 cccggctgca catgcgcagc gcgaagaagt gccgcagctc ccgggcgttc atggtcagca
     3001 tgatgcgggt ctcgcaggct cccggcagca cgaagcgcgc gtcctcgttg ctcttttcgc
     3061 ccgccgcgcc gagccgttcc tgccattcct cgtaccactg ctgcatttgc gccatctggc
     3121 cccggtattc ggcagctgcg tcctcgccca gcgccgcaat ggcgggggga acgatgtagc
     3181 cgaagccgtc cttatagctg acgtaccgct ggctctgcac gctgaagctg gcgatgcggt
     3241 gccgggtcag ctgggccagc agcacgcggc tgacgccctc gatcaaaaac gtgaaggaca
     3301 cgtgttccag cacgctttcg tgccccatgc tcatcagctt ttccaaaaaa gcctgctgat
     3361 cctgccggga caccttctcc atcagcttgt ccacattgcc cccggcgtag cacagccggg
     3421 ctcccagcgc gatctgcgcc tgcgcgtccg gcgtgtggct caaaagaagc acccgctgct
     3481 tgcactccgg cacggctttt ccccctctct ctgattgagt aacatcttat tttaatggat
     3541 tgccccggcg atgtcaaaca attcgccgat tctttccatc tgcccggaaa gatacagata
     3601 gcccatcagc gcctccaggc cggtggcccg gctgtagtcc tccggagact ggttctttgg
     3661 cacggcgtgt ttggcgtggg cgttctgccc ccggcgcagc acgtcccgct cctcctcggt
     3721 caggtggggc tcgatcttcc ggctggcctg tgcctgcgcc gccgcgttga ccagctgcac
     3781 cgccttccgg tgcagggctc ccgccttttc gccggaacgg agcaggctgc gccgcaccag
     3841 cagatcccac accgtatccc ccacataggc cagcgccaac ggcgttttca gccgggcctc
     3901 ccgggcggtc atcagggggg aaaacagttc ggtaggcatg ggctcctcct cttttccgtc
     3961 agtcatgggg ctcgtcccaa atcagggaga ccacgttttc cacgccgccc gcgtagcaga
     4021 acatatccac cggctgacag gcttccagcc ggtaaccgtc ctcccggagc agcttcacgt
     4081 cccgggcctg cgtaggcaca tggcagctca cgtacaccac ccgccggggc cgcgtccgca
     4141 gcaccgcgtc gatcaccggc tgctccacgc ccttccgggg cggatccagc agcaccacat
     4201 ccggacgaag gccccgctcc accgttccgg gcggcgttgc ggcgggcggc ttccaccgcc
     4261 tgcggcacga tttcgatgcc gatcaccttt tgggcgctgc ccgccatgca cagggaaatg
     4321 gtgcccgcgc ccgcgtaagc gtccacaacg atgtcctccg gtcgaagcgc cgcaaggcgg
     4381 atagcacatt cgtacagccg ctcgcactga tcggggttca cctgaaagaa ggaaaggggc
     4441 gcgatctcaa acgtcaggcc cagcagtgtt tcctcaatgg tatccgctcc ccacagtttt
     4501 cgggagctgc tgcccagaat gacgttattt cgggcccggt tcacgctcag gtgcagcgcc
     4561 cggaagccga gcatggcttt ctgcaaaagc tcggtcagca ttgagacctc cggcagcctg
     4621 tcggacgcgg cgaccagcaa caccatgacg tcgccccgcc ggttagaacg gatcaccaca
     4681 tgccgcaaaa gtcccttgcc cgtgacctcg tcgtagggct gcacccggca gcggctcatc
     4741 cacgtcttga ctgcgcccac gatggggctc agctccgtca tggaaatggg acagttctcg
     4801 atggaaatga tctggtggct gcggcggcga taaaagccca gttccggctc ccgccagctc
     4861 ccgcccaccg gcagggcggt tttgttgcgg cagtgccacg gctccgccgc ccctagcacc
     4921 ggcggcacct gctcggtcgc aagccccagt ccggcgatgc ggttcaggca gtccagcacc
     4981 tgcaggcgct tggcttccag cgtggcctcg taggtcatat gctggccgct gcagccgccg
     5041 catttgtcgt acaccgggca acgcggctgc gcccgctggg ccgacggctc cagcacctgc
     5101 accagccgac cgaaagcgta gttgggcagt gccttgacga tctgcacctc cgccgtttcc
     5161 ccgggcagca cccccggcac gaacacggcc atgccctcat gggaacaaac gccctccagc
     5221 tcgctgccca gccgggcgca ggaaagggtc agtcgttgat ttttctccaa agccccgctc
     5281 atgaatacca tccttccgtc ccgacgggtc agggcgtttt ttcgccggat acaaaaaatc
     5341 cggccgcccg cagaagaagg ccgcgtcaga cggcgctccg caggcaagcc ggaacgtgtt
     5401 tcagcagggt ttcccccggc agcaaatcaa aacccagttt gataatagca caacccgccg
     5461 ggggatacaa gagaaaatca gaagggtttc acggcgctca ggcccagcag cagctgggcg
     5521 gaataataga tgcccagact gaggtaatgc cacagggtgg gcttcttctg cacggtgttg
     5581 tagcacagcg tcatatcgga cgctacgaac agcgccgcgc cggccgccgc cagcacggaa
     5641 cgggtgccgg gagccaaaaa aggcagaggc agactgaacg ccagcagcgc cgacagcgcc
     5701 agtgcataaa tttgcagtcc cagcaggata aaccttgcgg gcactttatc ccggtagcga
     5761 tatacaaaaa tttccagcac ggcggcggcg atcacgaaca cggtcaggca ccaccagctt
     5821 agcacccggt agcgggacag ggccagcacg tacagcacgt gtcccagaaa gaacaggaat
     5881 cctccgatct caaagcgaat atccagcagc acgtcggcaa acacgcagac gcaaaggcct
     5941 agaaagatca gtctggcgta gtcgtcgctg ccgggcagga aaagcgccgc atacccggcg
     6001 aacatggcag ccatgatggt gggcagggct ttaaaaacca gggacagcac cttgtttttc
     6061 tgtttttttc cataaaagtg gaggggcata cacagtgccc acgtcagcag cactgtggcg
     6121 aggatcggga tcaaggcgtt ttcctcctta tttgattatt ccgaatccat tctggtgaac
     6181 ctatgatact gtttttaggg acgattgtca atcggaaagc cccgcccctt gtaggaaaag
     6241 cgggaaaatg gtacaataga cggccgaggg ggaacgaatg tgagcaaacg gacgggagcg
     6301 gcgctgtgcc tgatcggggc gctgtgctgg atgctggccg gtcttgccgg tacggtggtg
     6361 ggattcctga cctccgaggg gcgctttgag cgggcgctgc tccgacaggt gccgtgggag
     6421 gcgctgggca ttccgcagga ggaacttcgg gcgtttgccg gggagaccat gcggtatttc
     6481 cggggcgaga ccgaccgctg ggaaccagaa gtcaccgccg caagcgggcc gattgtgatc
     6541 tccgaggcct ttacgcggca tatggagacc gggcgaaacg gcatccgcac tgcccgggcg
     6601 ctggcaatcg caggcgcggt tctgggcgcg gcgctgatgg cgttggccgt ctggaaaaag
     6661 cgtttctcgc cggcggctta cgagctgggc gggtctctgc cgctaatgct ggccgccata
     6721 ctggggttgt gggccgcggc ggatttttcc gccatggggg aacaactgcg gcgggtccag
     6781 caggcggaan nnnnnnnnnt ggggctggct ccaccgtacc ttcatccccg atgggatttt
     6841 tcccgcttac gagccggtga tgctgctgtt tcccggcagt ctgttcgccg aatacctgcc
     6901 ccccgttctg acggccttcg ggctgacggc gctggccgtg ctgggtctgc cgccccttct
     6961 gcgggcggga tacagacggt tcccatggaa ccgttccaat gctgaaacaa aggatgaatt
     7021 gccatgaata ccatcccgga aatgcaagcc aagctggacg cactgcagca aaagcctgcc
     7081 gtgcgcattc tggccatgga atccagctgc gacgagactg ccgccgccat catcgaaaac
     7141 gggcggaaga tcgtttccag cgtgattttt tctcaggcgg acacccacgc cctgtatggc
     7201 ggcgtggtgc cggagatcgc cagccgcgcc cacgtggatg cgtgcgaccg ggtggtggat
     7261 caggcgctga aagaggcgga catggcgctt tccgacgtgg acgcgctggc cgtgacctac
     7321 ggccccgggc tggtaggcgc gctgctgacc ggcgtgaact gcatgaaagg gctcagctac
     7381 accagccgca tcccgctggt gccggtcaat catatcgagg gccatgtcag cgccaatttc
     7441 ctgaccacgc ctgatctgga accgccgttc ctctgtctgg tggtcagcgg cggccacagc
     7501 cacatcgtcc gggtgaacga ctatgcggac tataccctgc tgggccagac ggtggacgac
     7561 gcggcgggcg aagcctttga caaggcggcg cgggtgctgg ggctgaacta ccccggcggg
     7621 cccctgctga gcaggctggc cgagggcggc aacgctcatt tcctgacgct gccccatccc
     7681 catcccgacg gaaagtacga cttctccttc agnnnnnnnn nnccgccttt ctgaacgcct
     7741 gccacaagat ggagcagaag ggcgaggaac tcccccgggc cgatctggcc gcctccttcg
     7801 agcaggccgt ggtggacact ctgtgcgaaa aagcttcact ggctatggcg gatttcgggc
     7861 tgtccaccct gtgcatcgcg gggggcgtca gcgccaaccg gcggctgcgg cagcagttga
     7921 cggaactgag ccgcaagcag ggcttccgcc tgtgtatgcc ggaattatgg ctctgcaccg
     7981 acaacgccgc catgatcggc tccgccgcct attaccggct gcgcaagggc gagctggccc
     8041 cgttggaact gaacgccgtg cccgccctgt ccctttgctg aaaaaagaac gaccgtcctt
     8101 catattttcc gcttttctcc gccatactag agtgtgccgc ttacgcgtaa ggcagacggc
     8161 ccctcccggg gaaaggagca gaaaagatga aggacgattt ttatggaaaa aaacagccct
     8221 ttcgcagtcc gtggtggctg atcggcgcgg cggtgctgct gattgccgtg gcggctgcgc
     8281 tgctgtggcc cagtgaaagt ctggcccccg cgcaggagga gcagacccaa agcgccgcag
     8341 cggatcaggc cgcccggttg gaggacggcg cggagctgtt tcagcggttc acctacaccc
     8401 gctgcgacca cgtggtgacg cgccgggtca cggccccggt agagctgtac ggcaaaacgc
     8461 tggaagaagc gcagcccctc taccccgaat ggcgcatcac ggaattttct cccaaagcgc
     8521 tgcgaatgga gcgtcaggtg ccgctgttct gccccgatca tcaggtgttg atgcccaatg
     8581 ccgcagggac gctgtgcatc tatgaaaata aatacggcga tgcgctggcg ttggtcagcg
     8641 aactgtccgt cgccgtctcc gccctgcccg ccgcagcgca ggaggaagtg caggacgggc
     8701 tgggtttttc caccttggaa gaactggaag cctggctgga aaacatggag agctaagcca
     8761 tgctgaccgt ctttatccgc agcgttttgc tggtctgtct ggccgtctgc gccctgcggc
     8821 tgatgggcaa gcggcaggtg gggcagctgc agccctttga gctggtcatc gccatcatga
     8881 tcgccgatct ggccgccacg cccatggaaa gtctggaca
//
LOCUS       DAWAEA010000012         8778 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_1296_length_8778_cov_2.358936, whole genome shotgun sequence.
ACCESSION   DAWAEA010000012 DAWAEA010000000
VERSION     DAWAEA010000012.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 8778)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8778)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8778
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1296_length_8778_cov_2.358936"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    561..570
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1303..1312
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6105..6114
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7544..7553
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8072..8119
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1670 a         2559 c         2724 g         1737 t
ORIGIN      
        1 ggctgtacgt gggcggcctg accggtctgg taaagaagcc gggttccagc aacaacttcg
       61 ataccttcta tcataagggc acactgaaag tgcagcaggc tcccggcgcg gtgggctatt
      121 tgtttggcga tatggtcaaa gacggcatat atcagcaggc ctactatcac gggccgtcgg
      181 ccgcggccag cgagctgacc ggtcggcttg ccggcaattc cactttcgaa gcggcggtgg
      241 aggaagggtt ccaaccctat cagggagaag cgccgcttca gttcggtcag tcgaaaacct
      301 acaccgtaaa agaactggtt cccgaagggt acgaagccag ctacgtcacg gctgggggcc
      361 ataccattat caccaatatc aggaccaagc agccggacac ggctgaaatc gtaccgccca
      421 agactggcga ttcctttggg ctgtatacgg tgatcgcgct gctgttcctc agccttggcg
      481 cacttctgac gctggcagcg tcccggaaat cccggagctg atccaatgga aaagggccgt
      541 ccttcggggc ggctcttttc nnnnnnnnnn aatccgcaat cactttgcct atgcatggtg
      601 gcagtacgtg ctgctgatct gtctggcgat cttcggctgg aacctgctct acaccaccac
      661 ccgttaccgc agccccaacg accagcggct ggaatggtac tacggcggtt atcagctgga
      721 cggcgaaaaa aatatcgaca gcctgatgga cgacttgcac cagagccttt tctccgatct
      781 ggaggaagtg gaattccgca cggtaatgct ggatcagacc tacggctcca tgcagttgat
      841 ggtgtgggtc agcgcgggcg agggcgacct tttcatgctg gacagcgaat atttcagcca
      901 gctcgcccag agcgagggct ttgcggagct gaccccctat attgaaaacg gcacgctgaa
      961 cgtagacggc atggatctga gcgcgggcta tgtcatcaat caggatacgg gcaaaaagat
     1021 cctctacggc atcccggcca gccagctgcc ggggctgcag gaatacggcc tgatctgcga
     1081 cgatttctgt ctgggcgtga gcgtagccgg cgaaaacgaa gaagaagcca tccagctgct
     1141 gcaatggctg atcgacaatc tgcgcggcgg cagcgccgaa taatttttct atatttcagc
     1201 gcatcatttt tcctcctttg gggcatcctg tttcctgcgt ccgaaaaaag aatggaaaaa
     1261 gcaggaaagg aatgaaaaat gatgagtcca taccaaccgc agnnnnnnnn nnctttgcgg
     1321 ccgggaatat ccggcggggc tccacgtgat gggctgcctg atctgttttc cctgtgaaag
     1381 gaagctcctt cacccggaaa ccacgcctat tccccgcccg gttcggctcc gtctgaaccg
     1441 gctgtaccgg gagcgtatct gcgggttcta ggcggcgact gttttctctt ttgccgcctc
     1501 tattgaaagt ttttcggttt tttgttatgc tttgcaggtc gtatgattcc acacggcctg
     1561 tttttcattt ttagttcaag ggagaagggg cgaaaccggc atgggtcatt ttccttacga
     1621 cgcggtggtt ttcgatctgg acggcacgct gtttgacgcg gaggaaggca tcgtttcctc
     1681 ggtggtcaaa gccatgaagg aaatggggct ggaaattccg cagggcgctc agctgcggca
     1741 ggtggtgggg ccgcccctgc gttattcctt tcacgacctg ctgaacgtgc cttcggaacg
     1801 actggacgaa gctgccgacc gctacgccca tattttccgt tcggagggca tgtaccgcta
     1861 ttccgtttat cccggcatcc gcacgatgct ccgcgtcctg aaggaaaacg gcatttatgt
     1921 ggcgctggct tcttccaagc cccgtgatct gtgcgaacgc atcctgcgtc attacggcct
     1981 ccggcacttt ttcgaccggg tcatcggtga aacggacagc cacgccaagc tgggcaagcc
     2041 ggaaatgatc cgccgggcgc tgccggagcg ttactggcgc gccgccatgg tgggcgaccg
     2101 gctgtacgat atggaaggcg ccaaggctgc cggggtggac ggcgtcggcg cggtctacgg
     2161 ctgcggcagc gtggaagaac tgcaaaacgc cggagccacg ctgctggtca gcgacgcgga
     2221 cgagctgcgg gagctgctct gtccgggcgc tgccgtgccg cggggcttct tcctgtccat
     2281 ggagggccct gacggctgcg gcaagaccac acaggccaac ctgctggaac agagtctgcg
     2341 gcagctggga ttcgatctgg tgcgcactcg ggagccaggc ggctgcccca tcagcgaaaa
     2401 gattcgccag atcattctgg atacggaaaa cgccgaaatg tgcggcacct gcgaggcact
     2461 gctgtacgcc gcttcccgtg cccagcacgt gcatcaggtc attcgccccg ccgtggaagc
     2521 gggcaaggta gtgctcagcg accgctttgt ggacagctcc gtggcctatc agggcggcgg
     2581 acgggccatg ggcgtggata tgatccggca gatcaacgaa ccggcagtgg acggtatgct
     2641 gccggacgcg accgtttatc tggccatcga ccataccacg gcgatgaacc gccgtctgag
     2701 tgcctcccga cccgaccggc tggagatgga aagcggtgcg ttccacgggc gggtgcaggc
     2761 ggcctacgaa gaattgatca acagggaccg gcagcgcttt atcgtcgtta gcggcgacca
     2821 gccggtggag gagattgccc ggcaggtgct gcggcaggtg ctgaagcggc tggagccgga
     2881 ctgtgaacag gaggcgtgac caacgtggaa agaattgtca tcggcatgtc cggcggcgta
     2941 gacagctccg tggcggcgtt gttgctgaag cagcagggct acgacgtggt gggcgttttc
     3001 atgaaaaact gggaggaaaa ggacgagaac ggcacctgca ccgccgccga ggactgggag
     3061 gacgtgcagg cggtctgcga caagatcgac attccctact actccgtcaa ttttgccaag
     3121 gagtactacg accgggtgtt ttcctatttt ctggacgagt accgcaaggg ccgcaccccc
     3181 aacccggacg ttttgtgcaa ccgggagatc aagttcaagg cgtttctgga ctatgccatg
     3241 aagctgggcg cggagaaaat ggctaccggg cacttcgtcc gctcggaggt gcgggacggg
     3301 catcccgtac tgctcaaggg cgatgacccc aataaggatc agagctactt cctttatatg
     3361 ctcaaggagg cccagctggc caagtccctc tttcccgtgg gcggcatgac caaggcgcag
     3421 gtgcgggaga tcgcccggca gaacggtctg ccagtggcct ccaagaagga ctccaccggc
     3481 gtgtgcttta tcggcgaacg gaatttccgg cagtttttgc agggctttct gcccatgcag
     3541 cccggcgata tgcggacgga agagggcgaa aaggtcggcg agcacatcgg ccttgcctac
     3601 tacacgttgg gtcagcgccg gggactgggc atcggcggcc ggggcgacgg gcgcagctgg
     3661 ttcgtcatcg gcaaggactt ggagcataac gtgctgctgg tttcgcaggg cgaggatagt
     3721 ccccggctgt acagccgcta tgtgcgggct tcgcaggcca cgtggatcgc cgaaagcgcc
     3781 cccatggccg agggcgaaag ctgccgctgc accgcccggt tccgctaccg tcaggccgat
     3841 caggccgtca ccgtcacccg ccggggcgac gagctgctga tcgaggcaga cgagcttcag
     3901 cgggccgtta cgccggggca gagcgtggtg ctgtatcagg gcgacgtgtg ccttggcggc
     3961 ggcattgcgg aagaagcgtg gaaataaacg cctttcccta aaagccgtgt tgacctttac
     4021 ggttcacagg ctgcggcaaa ttttccattt ggggcttcgg ccagcggcga tcaatgggga
     4081 ttcctgctgt gctgcggctt atgagacaat ttcaacctat tcctttcaga atggcaaaag
     4141 tccttctgac tgcgctggtt gacctgcggt cggcaaatgg acccataaag gctgcgcctt
     4201 cgcaaacagc accgaagggt tttggcaagt ccaaagggcc gtttcccttt tcttggaacg
     4261 gcccttttcg ttggtcattt ttctactgca gccgggcggt gaaggtgatc agcgtaaccg
     4321 tcggcttatt gaacagccgc cccttcatgg ggtaatcatc actggccccg atgcccgggg
     4381 aaatatactg attcaggctg ttgatccgct gcatcccgcg aataccgtcg tccgagggga
     4441 accagccctt ttctggcaca tagatggccc ctccgccggg cagccgccac tggccgccgc
     4501 agtaatggcc gcacagcagc agcgaaatat tccgaaaatt gaagattttc tcctggtcgg
     4561 cccattcgat actttcccgg atataggagg tctccatggg cacgtggttc accgcgatct
     4621 gcaggtctgt gtccgtcatt tccttgagcg cctgctgggt acgctggtag gcctcgatgc
     4681 ggtagcccag cgcccggtac gccgcgccgc cctcggcctc atactgggtg ccctgcgctt
     4741 ccatgctctg ctgctggctg gtcagtgcgc cgatcaggcc gtccacgtcc acgtcgtaca
     4801 ggtattccgg ggagaaccac accgtttttt tgcccacggt ctgggagacc ggcgcgtcca
     4861 ggtagatcgc ccccgccttc tgcgcggcct ccacccagcc cgccagcacc ttcggcgttc
     4921 cccgggcggt ggaaagcacc ggctccgggt cctcgtcccc ggcgataaag taaatgggta
     4981 cgccctcccg cagggtattc aggttgtgga tcagcgtcag caggggttcg tcgtcgccgt
     5041 cccggcccac catatcgccg cttaatacca cggcatgata attcttcccg ttcagcaggg
     5101 atttccattg gctcagatca tccgccagtc cggaaccgtg caggtcgctg atgtgcagaa
     5161 cagtaaagcc ctcgaaggtc ttgtccagcc ccatgacgtg aaccttctcc gtctccagcg
     5221 acagcttctg gttcacgccc cggttggtca gccagacggt ggcggccagt gcggcaagca
     5281 gcgccagaac gaccagccac gcccccagat gcgagcgttt ttcccgctgc ggtgcgaaca
     5341 gatactcgtt tttacgtctt gccaaaagaa tcacccccac cttttctagc tttagtttac
     5401 gttgctttcc tgctccgcgt caatggagga tttctaagca aagtatgaca gttttccgct
     5461 gaaacaatac gaaaacgcag tattcgtaag aggtttgtca cagacggttt atacttgtaa
     5521 aaaagaaacg cgtctttttt cgttgtaaaa tctcctgcgg tctttctagg caatccccct
     5581 cgtctgtggt atactggaca ggtatattcc cctccgcgaa agttggcgaa cgcttatggg
     5641 gcgatccgac agcattcccc aaaggtagcc atggcgcgga ggcattcatt ctgctatgat
     5701 aaggtcagcc cgcagagatg cgggcggcgg aaaaggagct ctctttggcc aaggagaaaa
     5761 ccgtctacgt ctgtcagtcc tgcggctacg aagccgcccg ttggctgggc tgctgcccgg
     5821 actgcggaga atggaacact ttcgaggagc gccgcagcac ccctgctcct gctcccagca
     5881 aaaccgccga aaaagccgcc aaataccaga cgacccgcaa aagcgagatc gtggccctca
     5941 gcgaggtaga agaccggcag gagccccgct cctccaccgg caacgaagaa ctggaccggg
     6001 tgttgggcgg cggtctggtc accggcagcg ccattctgct gggcggcgac ccgggcatcg
     6061 gcaaatcgac gctgctgctt cagatctgcg cctatctcgg gaaannnnnn nnnnctgctg
     6121 ttgcaggtgg cgggcagtct gagccagcgc tgccgggtgc tgtacgtcag cggcgaggaa
     6181 agcgcacggc agatcaagat gcgcgccagc cgcatcggtg tgccccagga caagctgtat
     6241 gtgctggcgg aaaacgatgt ggagagcatt ctggaaaaat gcgagggaat gcagcctgac
     6301 gtgctcatca tcgacagtat tcaaaccatg gtgtccgagg acagcgccag cgcgccgggc
     6361 agcgtcagcc aggtgcgcca gagcgccgga gccatcgtcc gctacgccaa ggagaccggc
     6421 acgtccacct tcatcgtggg tcacgtgacc aaggaaggca gtctggcggg cccgcgcatt
     6481 ctggagcata tggtggacgc ggtgctgtac ttcgagggcg accggcagca tgagtaccgg
     6541 ctgcttcggg cagtgaagaa ccgcttcggc tccgtgaatg agctgggcgt gttcgaaatg
     6601 cgttccgagg gcatgattcc cgtccccaac cccagcgaga cgctgctgag ccagcgggct
     6661 cggggtgcgg gcggcagcgt ggtgttctgc acgctgcagg gcagccgccc cattctgtgc
     6721 gacctgcagg cgctggcggc caagtccttc tactccgtgc ccaagcgcac cgtgggcggc
     6781 atggattcca gccgggtggc cctgctgctg gctgtgatgg aaaagcgggc cgggaagaag
     6841 ctcttcgatc aggacgtgta catcaacgta gcgggcggtc tggccctgag cgaccccgcc
     6901 gccgatctgg cggtctgcgt ggccgtggcc tccagcctga tgggctttgc cctgtccccc
     6961 tccctgagcg tgatgggcga ggtgggcctg tgcggcgagg tgcgcccggt cagtcaggcg
     7021 gaacgccggg tcgcggaatg cgtccggctg ggatttacag agattctttt acccaagcag
     7081 aacatgaaaa gcatcaaacc catcgagggc gttcggctga ccggcgtgga cacgctgatg
     7141 caggcccttg cggtggtgac ggactgattc ccgctattgg cgggatgcgg aaaggcaggt
     7201 cgtttctttg aaaatgcgaa gcaaggccct tgaacggctt tcaagggcgc tgatcactct
     7261 gctgggggcg ggtctgggcg cggcgctggc catgctgctg actccgctgc tccggcgcgt
     7321 ttcccctcaa attttcgagc aattttccaa tcaggtgctg ctgtacgtgg tgctttgcct
     7381 gctgggcgcg ctgatctttt tcgccctcag catgtcgatc ctgacccgct gcatgaaggc
     7441 cgcggccgtc atcgagcagc actgggagaa aatgcccaac cggcagatcg tctggaccag
     7501 tttgggcctt ctgtgcggcc ttgccattgc ggcgctgttt accnnnnnnn nnnttaccca
     7561 gctgatcctg tcggcggggg ccagtcttct gacgctgtcc ttcagcgcgc tgacgtacat
     7621 ttttctgggc tatctgggcc tgcagatcgg caaccaccgc tatcattccg gcaagcatct
     7681 gccctccctg cgccgccgca agcgtcacac ggacggggat gaatccctgc tggaaaccgt
     7741 tctgtccgag gtttcggaag acggggatga aggcgaggac gagctgtccg ccggggagga
     7801 gtccgtgccc tgcaaggtgc tggatacctc cgtcatcatc gacggccgaa ttttggacgt
     7861 gctgaagacc ggctttctgg aaggaaaaat cgtcgttcct tccttcgtgc tggcggagct
     7921 gcggcacatc gcggacagcg gcgacagtct gcgccgcgcc cggggccgcc gggggctgga
     7981 cgtgctgaac cggatgcaga aggaactgaa ggcccccgtt accgtgatgg aaaaggacta
     8041 tcccgacatc gaagaagtgg acgtgaagct gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnt cgtagtggag aacggccgtc gccacgtggg cgagcaggtc
     8161 gcggtggtgg tgaccaccgt ccttcagacc agcgccgggc ggatgatctt ctccaagctc
     8221 aagtccgaag ccgaagggtc cgctgaagcc gccgctaaac gggcgatgta atcggaaaat
     8281 agaaaaacag cgctgccgaa gggaaatccg gcagcgctgt tttgatttgc tttatcgtac
     8341 ttccacccaa ttttgggccc gctccacggc ccggtgccag ccgtacaggg ccagatcccg
     8401 gttttgcgcg tccatctggg gcaggaaggt caggtcgggc ttcaccatgg aggcggcttc
     8461 ctccttgctg gcgtacaggc ccacgccgat gcccgccaac agggccgcgc ccagcgccgt
     8521 ggtctccagc accacaggcc gcaccacggg gatgcccaga atatccgact ggaactgcat
     8581 catgaagccg ttggcgctgg cgccaccgtc tacgttcatc tggacgggct tttcgccgcc
     8641 ccggtcggcc accatggcgc tgtagagatc gtggctctgg tagcagatgg cctccagcgc
     8701 cgcccggacg atgtgggccc ggcccgtgcc acgggtcatg cccacgatgg tgccccggct
     8761 gtacatatcc caataggg
//
LOCUS       DAWAEA010000013         8526 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_1349_length_8526_cov_2.387439, whole genome shotgun sequence.
ACCESSION   DAWAEA010000013 DAWAEA010000000
VERSION     DAWAEA010000013.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 8526)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8526)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8526
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1349_length_8526_cov_2.387439"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8037..8105
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1753 a         2470 c         2640 g         1594 t
ORIGIN      
        1 tgatgttggt ggcgtggtcg ccgatgcgtt ccagatcgtt gaccacgtgg aacagggagc
       61 cggtcaggtg cacgtcgccg gtgctgagct gcaggccctt gacctcgatc agctcccggg
      121 tgatctcttc gttcaggaag tcgatcactt cctcccggtt gtcaaactcc gccggttcca
      181 tggccttggg ggagaagaaa tagtccatgg cgaagcggta gttggtcatg acgatgtccg
      241 ccatgcgcat gacttccttg aaaagctgct cgaccgccac gggcggggtg gacagcaggc
      301 ggctgtcgaa atatttcagc cgcatgggct ccagaggctt gtcctcgccg cgtaccagaa
      361 ggtacgccag cttttccagc acgccgctca ggggcagcag caggagcgtc gtgaccagat
      421 tgaagcccac atgggcgatg gcaatctgca gacggatatt gtccccggca aacagggcga
      481 tccagtccgc cagcggcagg aacgccgcga ggatcaggaa gatcaccgtg ccgatgacgt
      541 tgaacaaaag atgcaccacg gcggcccgct tggcggtgcg gttggtgcca acggcggcca
      601 gcaggcaggt gacgcaggtg ccgatattct ggccgaacag aataaacagc gaggtattca
      661 ggctgacggc tcctgccgca gccagcgact gcagaatgcc gatgctggcc gcgctgcttt
      721 gcagaagggc cgtcaccagc gcacccacca gtacgcccac caccgggttg gcggcgtaga
      781 ccatcatatc ctgaaagccc ttccagtccc gcagaggctc catggcggag gacatcatgt
      841 ccatgcccag aaacagcatg cccagaccca tggcgatctg tccgcccagc ttgacggcgg
      901 ggcgattgcc cgccagctgc agcaccgcgc cgatggcggt gaagataatg gcgaagtcga
      961 tctccaccga cagcatcagc gaggtaatgg tggtgccgat gttcgcgccc atgatgacgc
     1021 cgatggcctg acttaattgc aaaagacccg cgttcacaaa gccgaccacc atgacggtgg
     1081 ttgcgctgct ggactgaatg acggcggtga tgaaagcgcc gatcaggaca gcggtcagct
     1141 tgttgcgggt cagaatttcc aaaaggtgct tcatgcggtt gcctgccgcg gtctccagac
     1201 catcgcccat ggtcttgatg ccgaagagaa acaggcccaa tcctcccatg agggacaata
     1261 ccatcgttgc gttcatgcgc aatcctcctg tgcatgtgcc gcgttccgtc ttccaacgcg
     1321 gccttccatt tttgctccga tttacaacgc ctagcagttt accatatgcc ctcccctgtt
     1381 gtaaagagtc ctggttttcc ctgtaacgtc cgcgaaaacg gtcgaagttt ggcggttttt
     1441 ggccgaacag gacaaaatgc gaagtttgta caaagaagca ccttgttttt tggtaaatga
     1501 aatcatataa tagaagcgtt gcatctgggt ctgtggttga aagccgatgc cagacgcggg
     1561 caaagcggtc cacgtaagcc gggcaaagcc ccggtgagca tggcgctttc tagaagtaag
     1621 tccggccgcc gcaagacggc gagagagggc gcgtagggcg ccgaagcgat gagcaaaccc
     1681 tccggccggc gagtgtgggg tcaaagacca ggtcagccag atgcaccaac ttaaacggaa
     1741 gtggaggatc cccaaaatgt accaggacaa aacgcttgtg tgccgtgact gcggcgctga
     1801 gttcgtgttc accgccggtg aacaggaatt ctatgcggag aaaggtttcc agaacgagcc
     1861 tacccgctgc aaggaatgcc gcactgcccg gaaggccagc cgtgccaatg cgccacgcga
     1921 atcctacgaa acggtgtgcg ccgcctgcgg caagcccacc accgtcccct tcgtgcccaa
     1981 gagcgaccgg cccatctatt gcagcgaatg cttcgccgct caccgtcacg gctgagtaag
     2041 ccgaaggtca gggaaaaatc ttttgcggaa gagttttttt caccgttgat tttcaaaaca
     2101 aggaaaccaa aacggaatca tcacgccccg ggcttcggtc cggggtttct gtttgccaaa
     2161 aagggtgcga agcaccgagg acgaggacag ccctgaaagg gctaccgcag tccgaagcct
     2221 gcgacgcgaa gcggggcagg ccgccaaaaa gggtgcgaag caccgagggc gagcacgccg
     2281 cgcagcggaa gcgcagtccg aaccaagcag tggtacacgc tgtatgtgac ccacggcgag
     2341 cacgccgcgc agcggaagcg cagtccgaag cctgcgatgc aaagcggggc gggccgccaa
     2401 aaagggtgcg aagcaccgag ggcgagcacg ccgcgcagtg gcagcgcagt ccgaagcacg
     2461 cgacgcgaag cggggcaggc cgctaaaaag ggtgcgaagc ccgtaacccg caggggaacg
     2521 ggctgtcgga gggcttttta cattcacttt ccctaaaaaa taaattacgt attgacaacg
     2581 gcactctatg gatataaact accgtctata tagaggattc tctatataga cggtaaagga
     2641 gtcgaaacca tgaactgctt tcaggcatcc attcccatta tgaaagccct ctgcgacgag
     2701 acccgtctgc agatattaag tatgctttcc gaacgggaaa tgaacggctg cgagatccac
     2761 cgggccttcg actgcaccca gcccaccatc agctaccata tgaaggcgct ggtggacgtg
     2821 gggctggtgc acgcccggcg ggaaggctgc gccacggtat acaccatcaa tcgagaaatt
     2881 tggcccgggg tagaggcgct gctgcacgcc atctgccggg ccgtgaaggg agaaaaggcc
     2941 cgtgagtgaa caaacgccgc tgctgcgact ggaaaacctg tgcaaggact atgggcaggg
     3001 gatgattctg catcacctga atctggatat tgaacaggga gaatttctga ccctgctggg
     3061 cccctccggc tgcggaaaga ccaccacttt gcggatgatc gccggactgg aaagcgtgac
     3121 cgagggccgc gttttgctgg atggacggga tattaccgat ctgccgccgg aaaagcggcc
     3181 gctgaacacg gtgttccaga gttatgctct gtttccccat atgaatgtgt ttcagaatat
     3241 cgcctacggg ttaaaagtaa agggagtcaa aaaggcggag cagaaggaac gggtggagga
     3301 agccctgcgg ctggtgcgcc tgtccgggta tgaaaagcgg atgccttccc agctgagcgg
     3361 cggtcagcgc cagcggatcg ccatcgcccg ggcggcggtg ctgcggccca aggtgctgct
     3421 gctggatgag cccctgggcg cgctggatct gaagctgcgt cagcagatgc agacggagct
     3481 gaagcggatg cagcaggaaa tgggcattac cttcgtttat atcactcacg atcaggagga
     3541 agccctgaac atgtccaccc ggatcgtgat ccttcgggag ggaagggtgg aacagatcgg
     3601 cacaccggag gaggtctacg agcatccgca gacgctgttc gccgccgggt tcatcggcca
     3661 gagcaacctg ctccggggcg aagtgacctc cgtccggccg gaaggccggc tgaccctgcg
     3721 tgtggaggat ggggagctgc ccgcaatcgc cgaccgtccc tttgccgtgg gcgaggaggc
     3781 ggtgctgtgc ctgcggcccc agcgggtacg ctatggcttc cagccccagc accagatgga
     3841 attgcagggc gtaatccggc agaaatacta ttccggcggg atgcagcata cgctgatcgc
     3901 cctcagcgac cggctgacgg tcaacgccgt gacacaggcc tccgaaatgg atcagttcca
     3961 gatcggcgac cgggtatacg tgggctggaa ccagacccac gcgccggtgg tgcccgacga
     4021 aaagctcgat ttggaagccg ccgagggggg aaagggatga accgtaagga caaacgcttc
     4081 gccctgctgc tgaagctgcc cctgtacctg tggacggtgc tgttcgtgct ggcggcgctg
     4141 ggctacgtca tcggcctgag cctgcagagc cggggcgagt tgatcggcgt gtccggtcaa
     4201 tggacgctgg acaactacgc ccgcctgcgg gaaccccagt attttcaggt gctggtgaac
     4261 agtctgcggc tggcggcgct gacgacgctg ctgtgcgccc tgatcgggta tcccttcggc
     4321 tatctgatgg cccggctgaa accggcttcc cgttccatcg tcacactgct gctggtggtg
     4381 cccttctgga ccaattccct catccgtatt tacggctggc gcattttgct catcggcaac
     4441 ggccccatca ataccctgct gatgaatctg gggtggatcc agcagccgct gaagctgctg
     4501 aacaccgagg gcgcggtgtt cctgggcatg gtgtacgcgc tggtgccctt catgatcctg
     4561 ccgtcctcca cggcggtgga aaagctggac tattccgtgg tggaggcggc ccgcgatctg
     4621 ggggccagcc cgctgcacgc ctttttcacc gtaaccgtgc ctctgaccct gtccgggctg
     4681 atggcggggt gcgtgctggt gttcatcccc tccacggggc tgttcttctt aaacgacctg
     4741 ctgggcggca gcaaaaccat gctggcgggc aatctgattc agagcctgat gaagagccgt
     4801 gacctgccca tggcctcgac cctgagcgtg ctgatgctgg ccgtcaccgg cgtggtcatc
     4861 gccctgtacc gcaaggcggg cggcgacgca gacggcctcg gcctgttttg aggaggaaaa
     4921 aacggatgaa aaaacattcc ttcggctcca ggctcttcct cggattggtg ctgctggtgc
     4981 tgtacctgcc cattgtggtg gtggtgatct attcctttaa cgacaattcc gcccggattc
     5041 cgctggcctt caccggctgg acgacccgct ggtacggcga actgctggac ggcaagggcg
     5101 gttatggccc agccctgctg ctgagtctgc gggtggcgct gtggtcagtg cttctgtcgg
     5161 tggttatcgg cagtctgggc gcggtgggca tgacccgcag cgctctgggc ttccggggca
     5221 gaccgggccg gttcgaccgc ttcatggagg ccttctccat cctgcccatc atgatcccgg
     5281 aaattgttct gggccttgcc ttcatggtta tcttcaacgt cctgggcgtt cgggacaacg
     5341 tggcccggct ggtgctggct cacaccacct tctgcattcc ctacgtgttc ctgtcggtca
     5401 aaagccggct ggtgggcatg gatcagtccc tgttcgacgc ggcccgggat ctgggcgcgt
     5461 cgccccgccg ggtgctgtgg gacattaccc tgcccctgtg ccgcccggcc atcctgagcg
     5521 gcgcgtttct ggcgctggcc atgagcctgg acgatttcgt catcagcttc ttcgtctacg
     5581 gcgcgggcga gggcacgctg cccctgaaaa tttattccag tgtgaaggtg ggggtcagcc
     5641 cccgggtcaa cgccttgtgt tcgctgatga tcgccgtcgt atttctggcc gtggcgaccc
     5701 agcggtacgt acaggccgtg cggaaggcaa gggaaaaacg tcaggcggaa tcgtaacgtc
     5761 cgcgtcccgt ctccggcggg agatccatct ctgtgatctc cgggctttgc ccgcgatcga
     5821 atagaacaat gcgtttccat ggaaactcag aaaggggttt tgaacatgaa aaaaggattg
     5881 tctctgctgc tggcccttgc gctggccgtg ctggctctgc ccgcgctggc cgtgggaacc
     5941 aagaccgtca acgtgctcag ctgggagggc tatgtggatg aggataccat tcaggctttc
     6001 gaaaatgaga cgggcataga cgtggtgtgg tcgcccatgg actccatcga cgacatgctg
     6061 ctgaaggtga cccagtccgg cggcgagggc tacgacctga tcctgaccag cgactattcg
     6121 ctggacatcc tgcgtcaggc ggggcttttg caggaactgg acaagagcct gctgcccaac
     6181 tacgaaaacc tgaacccgct gtacctgaac cagtacttcg acccggacaa tcagtacgtg
     6241 atcccctacg tggcgggctg cccgctgatc gtgtacgacc cggagcgggt gccctttgag
     6301 atcaccggct acgaagatct ttggaacgaa agcctgaccg actccgtggc ctgcttggag
     6361 cagtatcggg tgctgatcgg cgtgacgctg aaaaccctgg gctacagcat gaacgagacc
     6421 gacccggaca ttctggccca ggccagggaa aagctgatgc ccctgtacaa gaacatccgc
     6481 accttcggcg acatggaggc ctacgccgcc atgcagagcg gcgaggccag cgtgggcttc
     6541 ctgttcacgc cctttgccag cctgctcatg caggagttcc cccagtacaa gctggtgtac
     6601 cccaaggagg ggctgggctt cggtatcgac ggcttcgtgc tgaccgctgc ctcccagaat
     6661 gtggaaagcg cccatgcgtt cctgaactac ctgatggatg cgagggtggc cgcccacaac
     6721 gcggaatatc aggcctacat gtgcgtcaac aaggccgccg aggcggagct gagcgccgac
     6781 tactgggcca atcccgccta caatcccccg gaagaaatgc tgaaaaacgc ggagtatgtg
     6841 aagaacatcg gcgatgcgga atccctctac gcggacatct acgccgcctt taagctgcag
     6901 taacagccgc gatacggaag gaaggctcac tatgctgatt caaaacgcga aagcctttat
     6961 caacggaaaa tttcaggaaa agaccgacct tctgatccgg gacggacgga tcagcgccat
     7021 cggcaccggg ctggcggcgg agggcgaaga attgcttgat ctgggcggcg acctgctgct
     7081 gcccggcttt gtggacgttc atatccacgc cttcaagggc atggacacca tgcaggggga
     7141 ggaagccgtc cgccacatga gccgggaact gaaaaaggtg ggtgtggcgg cgttcctgcc
     7201 caccaccatg agcgccagcc cggagaacac ggttcgggcc ctcgcgggcg tgaaggccgt
     7261 catggagcgg cccgagcccg acggagccat cgtgctgggc gctcacatgg aagcgccgtt
     7321 tctggcggag gccaaggcgg gcgcccagct gaagcagtat tttgctgacc ccaacgagga
     7381 aaactggcag cgctataccg gcggactgga aagcgtcgtg cggctcatca ccatggcccc
     7441 ggagcgggcg ggggcgatgg agttcatctc cgccctgacg aagaagggca ttaccgtcag
     7501 catcggccat accaccgccg atgcggaaac cacccatgcg gcggcggagg cgggcgcaac
     7561 tcacgtgacc cacaccttca acgcccagac gcccctgaat caccgggccc ccggcgtgcc
     7621 aggcgcggcg ctggtggacg accggctcca gtgcgaggtc atttccgacg gcatccacct
     7681 gcacccggac attgtaaagc tcatcattcg gacgaagggc aaggaaaagc tggtggctat
     7741 caccgacgcc atggaagccg ccggaatgcc cgacggtcag tatcagctgg gcggtcagga
     7801 ggtctttgtc cacgaaggcg cggcccgtct gaaggacggc acgctggcgg gcagcactct
     7861 gaccatgatc cgcgctttcc agaacctgat acgtttcggc gcgacccccg aggaggcggc
     7921 gctgatgacc accaagaccc cggccctgtc catcggcaac gaagaactgg gcgagatcga
     7981 gctgggcgcg cccgcgatct tcagccgctt tgatgaaaat tacgccttca agggcannnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnngaaaa agttcatttt ccaacaaagc cggcggtgca gatgggaaaa ccatttgcat
     8161 cgctgctttg gatcagctga acgaaaaatt tttattttct aaaaaacgaa gggaaccgtc
     8221 tgacaggcgg ttcccttgct gtttatatac gctgcttttg atattcggtt ccccatgcct
     8281 ccatggaagc gatgatcggc ttcatggaca aacccagctc ggaaagggca tatttcactc
     8341 tcggcggcac ttcggggtag acggttcggg taatcagccc gtcgttttcc aaagagcgca
     8401 ggctgttcgt caatactttc tggctgatcc cgtccagatc ttttttcagc tcgttgaatc
     8461 gccagggacg cgccagcagg tttcggataa tgagcagctt ccatttgctt ccgatcaggg
     8521 aaacgg
//
LOCUS       DAWAEA010000014         8449 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_1366_length_8449_cov_2.407076, whole genome shotgun sequence.
ACCESSION   DAWAEA010000014 DAWAEA010000000
VERSION     DAWAEA010000014.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 8449)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8449)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8449
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1366_length_8449_cov_2.407076"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2045..2141
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1732 a         2480 c         2629 g         1511 t
ORIGIN      
        1 caacgacctg atcgcgcctg cgcggtcagc ggcgggattc tcaagggtta taacccttga
       61 gcgggggttt tcagggggca gagccccctg aagggccgtt gcacgaaaac gaggaacttg
      121 aggggctttg gccccgctcc ctccgttctt cctgcagcgg cccttttctt tggcacagga
      181 tatgcgaaga agggggaaac aaaatggcga cttatcatcg ggtcattttg aaactgagcg
      241 gcgaagcgct gggcgtggac ggacatggct tctgccatga aaaatttgaa gaggccgcgc
      301 ggatgctcat cgacgtgcag aaaaccggcg tggagctggc cgtggtcatc ggcggcggca
      361 acgtgtggcg cggccgccgg ggcgccgcca tgcgcatggg caccgtggcc gccgaccaga
      421 tggggatgct gggcaccctg cagaactgcc tgtatatgcg ggatacgctg gaacatctgg
      481 gcgcgaaggc tgcggtcatg agcgcactgg aaatgccccg gatcgccgaa acctatcata
      541 ccgtcgccgc gatggaaaaa ctccgtcagg gccagatcct gttgctgagc tgcggcattg
      601 gaaacccctg cttcaccacc gatacctgcg tggccctgcg cgccatcgaa ctggaatgcg
      661 acgcggtgct catggcgaag aatatcgacg gggtgtatac cgccgaccct cacgtagacc
      721 ccagcgccac cctgatttcc gatatttcct atggcgaagc cgccgaacgg ggcctgaagg
      781 tgatggacgc gaccgcgttc atcctgctgc gggataatca tgtgccgcag gtgcgcgtgt
      841 tcgggttgga gcctcccacc aatattctgc gggtgctgga gggcgacccc atgggcacgg
      901 tgctgcatcc ctgaccccga aagaaaaagg agaaacgaag aatggacacc aagcaaagcc
      961 ttgctaatgt gatcaccgag gccatcgagg cctgttaccc cggggtggac ggcctgccgg
     1021 atgcgtctgc gatcgccgac atgttggaaa ttcccccgca aaaagaaatg ggcgattacg
     1081 ccttcccctg ctttaagctg agccgcgccc tgcatatggg cccgccacag atcgccggaa
     1141 atctggcggg agccatttcc gccgggcatc tgtgcgaagc cgagcagcag ggcggctacc
     1201 tgaatttttt cctcaaccgg gataatttcg cccgggaaac gctggaaacc gtactgtccg
     1261 ccggggaaaa ctacggcgcg gggcaggaag gcgcgggaaa gaccatctgc ctggactatt
     1321 cctccatcaa tatcgccaag cggttccata tcggccacct gagcaccacc atgctgggcc
     1381 acagtctggc acgtatctac cgcttcctcg gctataagac ggtcagcatc aaccatttgg
     1441 gcgactgggg aacccagttc ggcaagctga tttatgccta caagacttgg ggcagccggg
     1501 aagaggtgga ggccaagggc gtgccggaat tgacccgcct gtatgtgaaa ttccatgagg
     1561 aagcggagca gcaccccgag ctggaagacg agggacgccg ctggttcaag gccatcgagg
     1621 acggcaacgc ggaggcgctg gaactgttca actggttcaa ggagctgacc ctgcgggacg
     1681 ccaagcccgt ttacgacctg ctgggcgtgc agttcgacag ctacaacggc gagagcttct
     1741 acgtggacaa gaccgaccgc gtggtggagg agctgcgcaa aaagaatctg ctgaccattt
     1801 ccgacggcgc ttccgtggta gacttggacg aggaaaagtc cggcatgccg ccctgcctga
     1861 tcctgaaaaa ggacgggacc accatctatg ctacccggga tatcgccgcc gccctgtacc
     1921 gtaaggagac ctatgatttc gataagtgcc tgtacgtggt agcctatcag caggatctcc
     1981 acttccgcca gtggttcaag gtcatcgaaa agatgggcta tccgtggtat aaggatttgg
     2041 ttcannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngaagggaca tgtgctcttt
     2161 ttggaggacg tgctcaacat ggccatcgag aaggctcggg ccattatcga ggaaaagagc
     2221 cccaatctgg aaaacaagga cgaagtggcc cggcaggtgg gcgtgggcgc agtggtctat
     2281 gctgacctgc agaacagccg catcaaggat atcgacttca gctgggatcg ggtactgagc
     2341 ttcgacggcg agaccggccc ctacgtgcag tacacccacg cccgctgctg cagcgcgctg
     2401 cggaaggctg agggtctgga acctgccgag ccggactaca gcgccctgtc cgacgactat
     2461 gcacaggagc tgctgcgcca gctgagccgc ttccccgaga cggtgcggga ggccgccaac
     2521 cgttacgagc ccagcatcat cacccgggcc gttaccgagc tgtgcaaggc ctacaacaag
     2581 ttctactatg aaaaccgcat catcgtggac gaggcccccg tgcgggaggc ccgcatccgc
     2641 ctgaccaaag cggtgaagga taccattaag accggtctgt atctgatcgg aatcgaagcg
     2701 ccggagcgga tgtaaaatgc tgctgaccag tacgaaaaat ccggtggtgc agtccgccaa
     2761 ggcactgctg accgccaaag gccgccggga gcaggacgcg ttcctctgcg acggcgagca
     2821 catgaccggc gaagccctgc gggtctgccc ggaaaaggtt tccgccctgt ttgtggagga
     2881 aggccagcag gagcggttcg ccccgttgct ggcgctggcc cggcagacgg aatgcgcggt
     2941 ctacgaagtg agcgcggcgg tgctggaaac catttcgcag gtgaaaacgc cgcagggaat
     3001 cgccgccgtc tgcggtctgc cgaaaccctg tccgctggaa cggcagggac aaaagctgct
     3061 cctgatggaa aacgtgcagg accccggcaa cgtgggcacc atcctgcgga cgctggacgc
     3121 ggcgggcttt gacggctgca tcctgacccc gggctgcgcc gatcccttcg cccccaaaac
     3181 catgcgggcc accatgggga gcctgttccg ggtgcccatg acctttgtgg aaaacagcgc
     3241 ggaggcggcc cgctggctgc ggacgaacgg ctgggccgtc atcggcgcgg agctgcgcgg
     3301 ccagcccttc tatcagcggg gcacccgccc ggacaagctc tgtgtgctta tcggcaacga
     3361 aggcgcgggg ctgacggagg aaattctcgc ccagtgcagc ttccgctata agctgcccat
     3421 gcggggcggt gcggaaagcc tgaacgcggg tatcgccgcg gcggtcatga tgtatgatct
     3481 gatgaatcgg taaatccaaa acgagggact gctttcctga aacggagaag cagtccctcg
     3541 ttttttgtct ttattcgtcg aaggaagaaa aggtgatctc atccccatcg taggagacgg
     3601 cgtatacacc cacgggttcc ggccagccgc actgttcctc caagcccagc gcctcgtcga
     3661 tctgctccca gtacaggtag tcgtcgatgc gcaggaacag gaagtcgcag cggttttccc
     3721 gcagcagccg gtaaatgttg tcggcggtca gctcgcccag acggtcgtaa tcctccacgg
     3781 gatactggac gatgtgcatc cgcatgggca gggcaaaata ttgataggcg tgggtcttgc
     3841 cgctgctgct tttgatctcc gcggatgcat agctgcagtc gatcagcagg gcgtggcggt
     3901 tggcgtattc ctcaaagggg aaagagtcca gctcgtcttc gtagtattcg atggcaaagg
     3961 ggcgaaggaa gccttcgtcg ttctggacgg gcaactgggc ggcgaaggcc tccggcggct
     4021 ggatcagcag catcaggggc aggatcgccg ctaccggcca atgggcgggc agcttcgccg
     4081 ggaaacgcca ggcctcgtcc tgccccgcca gcaggcggat cagcagaaac acgaagaaca
     4141 gcagtaccgg ggtgaacgcg gcgttcatgt agttcagcgc cacggcggcg atttcctcct
     4201 gcaccacgaa accagcaatc agcccaacgg tgtagcacag cgcggctacg gccatggcca
     4261 gcagcgcgcc cagcgttcgc cgacgctggg gtttgggctg aaagaccgcc agcacgatcg
     4321 ccagccccac gcccgccagc agccacggat aagcgctgtg ccagagggaa tcgccgaatt
     4381 gggcgaccaa cggctgcaac cgcgcccaca gaccgctgcc cagggaaccc atcaattcgc
     4441 cggttcgggg attgccgccc agcagactgg cgagggaagg cgcactgaca ctggaaacgg
     4501 caggggtcag cccataggcg gctttcgcca gctccgcctg ttcataggcc agctgggaat
     4561 gatagtggta caaaatcgac catgagaaat gcacgatgac gggcaggagc acagccagcc
     4621 agctcatcca gcgcagcgcc cggacgggct ggccggatct tttcacgccg gggatggaaa
     4681 acagcatgga cgcgccgatg accagcgcga acaacaggcc ggaatagatc ttcatgccgg
     4741 tcaacaccgc cgcggcgcac agggagggca gccacgaggc gggccggtcg tccctgcgga
     4801 agaacaggca caggagcccc gccgtcagca gcgccgccat ggggccgtca ccccgggtgg
     4861 aaaagacggt cagcccaaag gccgagaaca gataggccag cacggccccg gtcagtgccg
     4921 ggagccatgt gccttccagc agatcgccga tggccagaca ggcggccaga atcgccgatg
     4981 caatcaccat cagaagcccc gcgtcctgcc agccgaagac ctgcaccaca cagtattgca
     5041 gcgccgccag catggggaaa tagtcggtgt ggctcatgtt ggtatccatg tggatgggga
     5101 aacgcccgtc ggtgtacagc tcccggacga tgcgcatcca gtaggaatag gagtcatgat
     5161 cggtgatcag cacgtccttc agcgcaagga aacatccggc gcataaaagc agccagatca
     5221 gcagggaagg ccgcagcacc cgctgccgaa gttgccgctt gttcaaaagc agcagcacca
     5281 gccctgccgc cagcgcggcc agaccgcccg ccatgagaaa ataggccgtc acctgccgca
     5341 gcggcagcac gatcacgccg agataggccg tcagcagcat cccgcatacg gcgcacagca
     5401 gcgcctggcc cagcctcagc gggcgcacca gcgccatcca cgccagcgcc cagcccaaca
     5461 gcgaggcaaa ataccatcca tctgtcattg cattccgttc ccttctgggt gagataggaa
     5521 aaagggactg cgcagccgcg cagtccccca gagaataggg ccttagttcc cgttcgtagc
     5581 ggtcagcagt tcgatcacgc tggagggcag tttgctcagc acggtgtaga gcacctgcgt
     5641 ggcgttggtg ctgatggagg taagatactc ggtcagttca tcatcgctca tctgcagggg
     5701 ctgcacggcg ttttccgcgg tggcggattc gatggccttc agggcgttgt catccgcgtt
     5761 ttcggaggtg gtcacccgca gcgagaacag cgggcgctcg gaagcggcca gctccgtaat
     5821 ggccacggcg ttctcccggg cgtacaggga gacaagccgc tcggtctgat cgccctcggt
     5881 gctgccggta taggtgaagg tgatctggtt ttcgtccgcc agcatggcca gccagccgtc
     5941 ggttttgctg tccgcactgt tcagcacgcc cttgacggtc aggatgggct tttccttgcc
     6001 gtcgtccact tcgttcatag cgccgtccag cgtataggag ccgtcctcct cggtcagcac
     6061 gaaggccaga tcggcttcgg tctcgccgtc ctcgcccagc atcatggtgg cgctgtgatt
     6121 cacaacgccg tccttcgtca ggcggttgta ggcggcggtc atctggacgc tttccctttc
     6181 ggcttcgccg tcatcgtccg tcacggtcat ggcggaatcc atggtcattt ccagggccac
     6241 cagatgactg tcgtcggcat acatggtcat gaccacgtcc atcttttcca tgttgtccat
     6301 ggcctttttc agctcggcag cggcttcttc ctcggtcatg gagccggagt cggtcagcat
     6361 tttctgaacg gtggtgctgc tctgcagcgt ggccacgtcg tccttggtca gggtgatctt
     6421 gatgctctga gtggcagggt cgtgagcttc gctggtaaag ctgccctcgg tgatctccgc
     6481 ccggtccaga atgcccagca cgtagtccgc catcgcttcg tcgtcggcaa aggcttcctt
     6541 gatctgagcg cgcatttctt cgctgttgaa cgtctcgtcg ggcaggttga cggagggatc
     6601 ggccagcgcc gtccccttca gctcgttcat cataccggcc tggctgaaaa gctgggcgat
     6661 catgggcttg atctggctga caatttcttc gtccgcgctt tagtcggcag tcgccagctg
     6721 ggtcagcacc tgaccgatgt cctcggcgga ggcatagacg ggtttgtcgc ccagaatggc
     6781 gctgtccaga tacaggccgt cgccggtcat gcgcgtggcg gcgctgaaaa cgtcctcgcc
     6841 ggacaggctc agcgtcagca ttccgctgcc ttcgcccttc agaaagcgca cggtcagaac
     6901 gtcgaacaga tctttgatcg cgtccacaga cgccatatcg tcgccgggga tcagctgaaa
     6961 gctgtactcg gtagcggcca tggcgctcag gggcagcatg gcgcacagca gcagagccag
     7021 aaggactgcc agcagtttgt tttgtttcat cgttggtttt ctcctttcaa tgaccgtttc
     7081 gaacggttcc tcgaaaccat cttcatcata caacattcgg aagcgtttgt catgtgtttt
     7141 gggctgaaaa ccggcaaatg gagtgtaaaa ccttcctttt cggcgctgaa cgcacccgcc
     7201 gcaggggaaa aacagggtaa attctggata atccgccgcc tgtatgctat actagccagg
     7261 ataagggagg gatggtatgc gctttaccaa aatggaaggc attggaaacg actatattta
     7321 tatcaacggt ctggaggaaa ccattgccga cccggcggcg ctgagcgtgc ggatgagccg
     7381 ccttcatttc ggctgcggct cggacgggct gattttgatt ttgcccagca gtcaggcgga
     7441 ttttcggatg cagatgttca ataacgacgg cagcgaaagc gagatgtgcg gcaacggtat
     7501 ccgctgcgtg gccaagtatt gccacgaccg cggtctaacg gacaagaccg acctgaccat
     7561 cgaaacgggc gcgggagtga agcgactgca cctgaatgtg gaaaacggcg agacggtcag
     7621 cgtgcgggtg gatatgggcc tgccggagtt gaacggtcgg aagatcccta caacggcgga
     7681 cggagaaccc gtcgtcggcc atgtgctgac cgccaacggc catgaatacc cctgcacctt
     7741 tgtcagcatg ggcaatcctc atgccgtcac ctttgtggac gaccccgata ctgcccccgt
     7801 gaccacggac ggcccggtgc tggaatgcga ccccgtcttt ccccgaaagg ccaatatcga
     7861 gtttgtgcag gtgatcgacc ccacccacgt gaaaatgcgg gtctgggagc ggggcaccgg
     7921 cgagacgctg gcctgcggta ccggggccag cgcggtcatg gtggcctgtg tgctcaacgg
     7981 gctgacggaa cgccatgcgg agatcgccct gcccggcggc aagctgaatc tggagtggaa
     8041 cgagcaggac ggccacgttt acatgaccgg ccccgccacg ttcgtgtacg acggccagtg
     8101 gctgcaggac tgaaccccaa gacagaccct tcgcgcaaga cagatgaaca acaaggagga
     8161 accaaatcat gctgaaggtg aatccccatt atgaaaagct gcccggcagc tatctctttg
     8221 cggagatcgc ccgccgggtg aaggaatacg gacaggagca tcccgacgcg cagctgatcc
     8281 ggctgggcat cggcgacgtg actcgccccc ttgcccctgc cgtggtggag gccatggaga
     8341 aggcggcccg ggaaatgggc aaggccgaga cctttcgggg ctacggcccg gattacggct
     8401 atgattttct gatccaggcc attctggata acgattataa aggattggg
//
LOCUS       DAWAEA010000015         8318 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_1408_length_8318_cov_2.761346, whole genome shotgun sequence.
ACCESSION   DAWAEA010000015 DAWAEA010000000
VERSION     DAWAEA010000015.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 8318)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8318)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8318
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1408_length_8318_cov_2.761346"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2492..2501
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3852..3861
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6424..6433
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1623 a         2668 c         2428 g         1569 t
ORIGIN      
        1 gtctcggtgg gcagcccctc cgccctggga tggtgcggat cgaacaccgc cgccatttca
       61 tacagagaag cgctctcggt ggcgtggttc atgttgaagg acagggtctt gagcatgtcg
      121 cagaccagcg tgggccgcat gacggcggtg tcctcgccca gcggattgcg gatggccatg
      181 ggctgcatgc gcaggtcgct ttccggcaga cccagcttgg cgatctcctt ccggccggtg
      241 aaggaatagt tcatgatctc cagatagccc atgccctgaa ggagacggcc cagcttgttt
      301 ttcagatgct tcatggggct gacgccgccc tgcgtggtgc tgcccttgag gggcgtagcg
      361 ccgatgtggt cgtagccgta catccgcagg cattcctcgc agacgtccgc ctcgccgtcc
      421 agatcctccc ggaagcttca cgctggcagt gatcacctgc tgaggcttgg gttcaggata
      481 gaggtcgatc acgccgctga ccacgtcgcc cgcgttcagc acgttcacca gatggcaggc
      541 gcgatccagc gcgtccatca cggtggcggc gcagacgccg cgctcgaacc ggccgctggc
      601 ctcggtgcgc attcccagcg cgcggctggt aaggcggatg gccgtgcggt cgaaggcggc
      661 gcattcgaac atcacgtcgg tggtgccttc gatgatttcg ctttcctcgc cgcccataat
      721 gcctgccaga ccggtagcgt tgttcgcgtc gcagatgcac agatcatccg tccgcagctt
      781 ccgctccaca ccgtccagcg tggtcagcac ttcgccctcg cgggcccggc ggacgatgat
      841 ttcccggccc gctaccttgc tcaggtcaaa ggcgtgcatg ggatggccgt actcaatcat
      901 gacgaagttg gtaatgtcca cgatgttgtt gatggaacgc agacccgccg cgtgcaggta
      961 cttccgcagc cacaggggcg agggcgcgac gcgcacgttc cggatgaccc gggccgcgta
     1021 gcgggggcac aggtcgggcg cttccacgcg gactttcact tcgtcgtgaa tgtcgccgcc
     1081 cgcttcggtc acgtcggtct tgggcgcttt gaacgtggtg tccagcgccg ccgcggtctc
     1141 ccgggcaatg cccaccgcgc acagacagtc gggacggttg gccagaattt cgaaatccac
     1201 ggcgtagtcg tcaatgccca gaatggggcg cacgtcgctg cccacggcgt attcttcgtt
     1261 gaagatcagc aggcccgcat cccctacgct ggggtacagc tcctgcggca ccttcagttc
     1321 cgtagcggag cagagcattc cctcggagac catgccccgc agcttgccct tcttgatctt
     1381 cacaccgccg ggcaagtgag agccgtccag cgccacgggc accagcacgc ccaccttcac
     1441 attgggcgcg ccgcacacga tgtgcagggg ttcctcgccg cccacatcca cggtgcactg
     1501 gtgcagatgg gtgccctcta cgtcctcgca ggtcagcaca cggcccacga ccaccttgtc
     1561 catgccgtcg gccacgtcct ccacgccctc cacggccgtg ccggacatga tcattttgct
     1621 ttcatagtcc ttgggactga cgggaatgtc cgcgtattcc cgaatcatat tcatacccag
     1681 tttcatcgtt cattccctcc ttagcgaagc tgtcccagca ggcgcgcgtc gccctcgtag
     1741 agcagacgca tatcggtgat gccgtagcgc ttcatggcga tgcgctccag accgatgcca
     1801 aaggcgaagc cggaatactt gtggctgtcg ataccgcaca tttccagcac cttggggttc
     1861 accatgccgc agccaagcac ttccacccag ccggtgtcct tgcagatgcg gcagcccttg
     1921 ccatggcagg cggagcaggt caggtccacc tcggcgctgg gctcggtgaa ggggaagaag
     1981 ctggggcgga agcgggtgga aacatcgttg ccgtaaagct tcttggcaaa ggcttccagc
     2041 gtgccgcgca ggtcggacat gctgacgttt tcatccacca ccagaccctc catctgatgg
     2101 aacacggggc tgtgggtcgc gtccacctcg tcggcgcggt acacgcggcc ggggcagatg
     2161 atgcggatgg gcggcttgcg ggtcagcata gcgcgggcct gcatggggct ggtatgggtg
     2221 cgcagcacaa tgttgtcgtc catatagaag gtatcctgcg cgtcccgggc cggatggttc
     2281 ttgggcaggt tcatcagctc aaaattatag tgatccagct ccacctcggg accttccatc
     2341 acctcgaagc caaggccggt gaagcagtcg atcagctcgt ccatcaccag cgtgatggga
     2401 tgggcgctgc ccaccttcac agggcggcgg ggttcggtca cgtcgatggt ctccgccttc
     2461 aaagaggctt cccgctccat cttctgcagc annnnnnnnn ngtagcggat gtccttcagg
     2521 tccttcttga gcagctcggt cagggtgccc tttttgccca gcactttgac gcgcagcgcg
     2581 tccagcgcgg cggtgtcctt catttcggag aggttctgtt ccacctgctc ccgcagttcc
     2641 cgcagagtat ctgtcactgc catgataggg ttcctccttc tatcgtttca tcaaaatgga
     2701 atgggaatgg tgaagttcat gcttttccca gcaggtcggg gatgtcggat tccccgtata
     2761 cggggacgcc gcgctgacgg ctgaccaccc ggtctccctg ccgctcgctg ggagaccggg
     2821 gcgtcggcag accgcccagc tgttccgggc agacagggat cagccggtat cgcccggcca
     2881 gttcttccac gccgggctga ggcttgcttc cgccgtcgta gcggcacctc acccccagca
     2941 gacaggcgct gatcagcagc gttttcatgc cgttctcctt tcctgcatcg ccgttttcgg
     3001 ccatcaaaaa agccccgtcc cctcatggga cgaggcctga acgccgcgtg ttaccaccca
     3061 aattccgcac gggccgcagg gccgatgcgc tcttcgcctg taacggggcc tgaccgtttc
     3121 cccttactga aaaaggttca ggggaaaagc tcggaaggga atttcccgca gccaattcgg
     3181 acggcttgca gcaaggaacg cggagttcct gccgtcctct ctgagaatcg ctgtgcatcg
     3241 ggcgtttgtc ttcgtcatcg ccatgcgtcc cgaagccgga acgcggtgct attatagcac
     3301 ggaagccggg ctttggcaag gggcggcgcg cttttcggcc agcagacaga gggcggccag
     3361 ccacagcggc agggacgcca tggcgggcag cagataacga atgtacacct ccgggcagaa
     3421 cagggtggcc gccgccgtga tcgccgaggg aatcatcgta agcagcgtgt ttctccgccg
     3481 cagaatccat gaaacgtaac caagcagaat catgccgttg acgcagaagc ccatgctggc
     3541 aaaattcccg atcaggggca gccggtcgaa ccggaagtac cattccgtca gcgcccgctg
     3601 ccaactcatc agggggcgaa tctcttccag attgaagaac cgggtctcgt ggaaggcgta
     3661 gggataggtg tactgctgaa tatccgcgta gctgtgatag gtgggcacgt tgtgctgcag
     3721 gtcgaacatc cagtagttca tgttcagcat cgcgtccaga taatccacgg gatactgacg
     3781 gagctgcttc caccagaacc gcagataggc ctccggggaa tgctttcccc gctccagcac
     3841 gtccaccgtg gnnnnnnnnn ngggacgtac cgctccgcca gctgatcgta agcgatcatc
     3901 gtatcgacgg cggctttttc ctcatccgtt acgccctcgc cgccgtaaaa cttggccact
     3961 cgggccgtct gctggaacgg gatggacaga tagtcataga atctgacctc ttccagcccc
     4021 aaaaatcgcg tcgcggcagc gttggctccc gcgctcaggc cgaaggccag cgcgctgcac
     4081 accagcagac aggggatatt ccgccgcatc gccgggcggc ggcaaaggac gatcagcatc
     4141 gccacaaggc agggaactac cacggacacg ccgttgtagc gagtcagcac cgccagcagg
     4201 ctgtccgctg tcagcagtac ccagcgagat ttcttccggc agaaagcctc cggctccgcc
     4261 agaaactcca tcatcagcac gcccaacagc agcatccaca ccatgtactg cgcatccttg
     4321 cccagcgtgg taatccagcc gatataaata ggattgactg cgaacagcgt cagcgccaga
     4381 agctgcaccc agcgcggcgc ttttcgggtg tgcatcagcc acagggcata gctcaatgtc
     4441 agcatcaggc tgacagactg gaacagcatg attgcaaagt atgcccagtt cacattgccc
     4501 agcttttcgc ccagcaggaa cgcgccgccg tacagaagcg tccccgtggg cggaaaggtg
     4561 ttgttcttcg gcgaaaggcc gagaaccatc cgaatgggca tgaccacatc cagatgaatc
     4621 acgcccggat atttgatcgc gatctgccag ccgtaagcgg ccagcagcac cagaaaggga
     4681 aactcgaagg gatgcctgtc ccagaaagcg cagaagcgtt cctcttttcg gggacggctc
     4741 aacccctccg ccaacgcccg aagggcatac aggaaccaaa gagacatgcc cacaaaaatc
     4801 aacaggcttt tgaacagctg gaagctgttc tgccagatca gacgaacggt ctccccggcc
     4861 cgcatagcgg cgcacagcag cgctatgccg cccagaaaag cgctcagcag gtactcgccc
     4921 gcctttgcgc cctcagcccg gcgaagcaga taccgccggg ccagccagaa cgccagcagc
     4981 agtacgcagg tcgcggccag cggctccaga gtgaacgaac gggacgtgcg cccgatcagc
     5041 tgatacagaa agcctcccgc gtccaccgcg tccgggttca gctttcgagc tggcgtcata
     5101 tccagcccgg cggcgagggt catcaaaagc gctgccagcg cggcagcgcc cgtccgaagg
     5161 gtctttggtg actttttcaa aagcgttttt ccccgctttc ccctgtttta ttctgccgcg
     5221 ctttccttca cccggcggaa tttttctcga tccagatggc ggacgatggc aacgccggcg
     5281 atcaggccgc agcccatgcc cacgctccgc agccctgtca cggtataata cgtacaggcc
     5341 gccgcagcaa agaccgcaaa ggccacaatg ctgcgaacgc ggttgggctt aatgacccag
     5401 accacagaaa aaaagatata gggcacggcc agaagcagca ccgtgggcat gggcagcagc
     5461 gccagcagca ccccaaaggc ggtggcgatg cacttgccgc cgctgaaatg gtcaaagggc
     5521 gcggtggcgt ggcccagcac cggggccatc ataacggcgg caaactgcat aggttctatc
     5581 cccacggtat gcgccgccag atacacgggc agaaagccct tggacagatc gaaaaacagg
     5641 cacagcagcc ccgcgggcac gccgcagtgc tgaaacacgt tggccgcacc tgggttatgg
     5701 tcgtcgctga gggcgcacac gtccttgtgc agtaccagct tggggatcca ccggcagaac
     5761 atgatgctgc ccagcagata cccggcgacg atatgcagcg tccatacgac catgggatcc
     5821 tgcgtctgca agtagcggat catcattggt tcaagataaa ctgcgcgatc gtgtgggccg
     5881 cgtgagccgg gagcgtctcc cgctgggccc tgcgcatcct ttccgcccgc tccggatcga
     5941 tggcgagctg tatggcgaag gccacggcct cctcgtcgct ttgggcattc agcgccatgc
     6001 cgtgctgctg gaagaacgcc gcgttgcagg tctcgcagcc gggaatggcc ttcaccgcca
     6061 caaagggcac gttagccgtg gcggcctccg tgctggtcag cccgccgggc ttggaaagca
     6121 tcacgtcggc ggcccgcatg tataccggca cctgatcggt aaagggaacg ggccgaatct
     6181 gcccctccgc gccgtagcgc tcccgaatcg tctcacccag gctttcgtta tgccccacca
     6241 gaatctgcgc cagcatggtc tcgtctgccg cagccagaaa agcgtcgcac agccccagca
     6301 ggctttcgca gcccacgccg ccggtcatga tgaggaacag cttcttttct tcggggattc
     6361 ccagtctggc ccgcgcctcc tgccggggca ggggggaatc gaaggccggc gaaacgggaa
     6421 tgcnnnnnnn nnnccgaagg gatgcagctt gtcagcgggc aggccccggc ggatcagatt
     6481 ttccgacagg ctggcatgag gggtgaaata cccgtccagc tctgtttccg ccagaaaagg
     6541 agtgcaggta tagtcggtca tgatcccata ggcaggaacg gtgcttttgc cccgcttttt
     6601 cagagcggtc atggcctcca tgccgtacag gtgggtgcag actacatccg tatattgttg
     6661 cgcctccaaa aaacgattca gccgctcggc ataggccgcg ttagccagat acaccggcga
     6721 caaccagccc gtagcgtcgt acagcgctcc cgctttatac agcgcgccga aagccttggg
     6781 cgtgcggcgg atcataccgt tatagaagga agaaataaaa tgctgggcgt gttttccctg
     6841 aaaagaaacc gggtccatga tccgggacgg cacctccagc gcatcgaagg cgctatgcat
     6901 ggctttggca gcgctgttgt gcccctcgcc cgtaaagcag ctcaaaatca ggattctccg
     6961 ctccaaagtc gaacctcctt tcaggatcgc gtcaggcctc ggccgcaggc agcgcggtgg
     7021 cctgaacctc catcacgtgg cggccgtcgg tcttcagcac gtcaaaccgc cacttttccc
     7081 actggaagga atcccccggt ttggggatat tctcggtctg ctccatcacc cagccgccca
     7141 cagtgctggc gtccgcgtct ccgatctcct gatccagcgc gtcctccagc tcgtccagcg
     7201 gcgtatcgcc gaggatcagc agagagccgt ccttgccatg gcggatgttg acctgcacct
     7261 gatcgtgctc gtcccaaatt tcgcccacca gttcctccag aatatcctcc atggtgacga
     7321 taccggcggt gccgccgtac tcgtccgcca ccacggccat ctggctgcgg gtgcgctgca
     7381 tcagcttcag cgtcgtgctg acctgcgccg tttccggcac gaacaggggc ttcttcatca
     7441 gctcctgcag ggtgcagggg cccttctgca gccggtggaa gaaatccttc tggtgcaaaa
     7501 tgcccacgat atgatccagc gattcctcat acaccggcag acgggagtgc ttgctttcct
     7561 gcaaaatcgc caccacttcc tcctgcgtgg cgtccagcgg cacgccctcc accttgaccc
     7621 ggggcgtgag gatatccgtc aggctgcggt cgtcaaacag ctcgatgctg tttttcagca
     7681 ggtcgccctc gtcctcgttc atgccgccct cctgctgcac ctcgtccacc agcgtcagca
     7741 gctcgtcctg cgagaaggta cggtcgtttt cgaaatggaa catgcggcac agcagctttt
     7801 tccacccgga aaacaccgcg ttgatgggct tgagcaccac cagcagcacc cgcatgaccg
     7861 gcgttacggc catggcgaag ttttccggca tttccttggc gacgctcttg ggcgtgatct
     7921 cgccgaagat cagcaccacc accgtgcaca cgatcgttga aacggtcggg cccgaagacg
     7981 aacccagtaa attcacgaac agcaccgtgc cgatagaggc catcgaaata ttgacgatat
     8041 tattgcccac cagaatggag gacagcagac tatcgtaatt ttcagccagc ttcagcgcgg
     8101 cctcggcccg cttattgccc ttttcggcca tatttttcag ccgcgtccga ttcagcgcgg
     8161 aaaacgacgt ttccgtcgcc gagaaaaacg cagacatcac cagcagaacg accataatga
     8221 ttccctgaat caaattccca tccacagaaa aaacctcctg gcaggcaggg gcagtgcccc
     8281 tgcaccccgg actgcgcgct atgcgcgcag ggtactag
//
LOCUS       DAWAEA010000016         8270 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_1420_length_8270_cov_2.205843, whole genome shotgun sequence.
ACCESSION   DAWAEA010000016 DAWAEA010000000
VERSION     DAWAEA010000016.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 8270)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8270)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8270
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1420_length_8270_cov_2.205843"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2134..2143
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4579..4588
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5069..5078
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6139..6148
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7416..7425
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1688 a         2545 c         2474 g         1513 t
ORIGIN      
        1 gtaccgacta cgacctgacc cagcaccaga atcattccgg caagagcatg gaatatctgg
       61 atcccgtcac caacgagaaa tatatcccct actgcatcga gccttctctg ggcgtggacc
      121 gtgccgtgct ggccttcctg tgcaacgcct atgaggagca ggagctggag ggcggcgacg
      181 tgcgtaccgt gctgcacttt catcccgcgc tggcgccctt taaggccgcc gtgctgcccc
      241 tgcagaagaa caagctgggt gcgctggcca gcggcatcta cgagaagctg tccaagagct
      301 tcatggtgga ctacgacgag accggctcca tcggcaagcg ctaccgccgt caggatgaga
      361 tcggcacgcc gttctgcatc tgcgtggact tcgagaccga agagaccggc accgttacca
      421 tccgcgaccg cgacaccatg cagcaggagc gcgtgaaggt ggacgatctg gaagcctaca
      481 ttgcccagcg ggtgaaattc taaatcgtgt cccctttttc gggcgtggtc aggtttctga
      541 ctggctttca ccgcccattt ttgaaaaccg aacaaacggg ttccggcggg aaaacgtcgc
      601 cgggctctcg aacctgcgag ggacggctct gtgaatgggc cgtcccgtcg cgtgaaggag
      661 gatttccatg tccctatccg cttgggatca acgccagagc cgatggaacc ggcttcatct
      721 ctgcgttgtg ctgctgtggt ccatggctct gctgttggag cgcttcgtaa cggaaagagg
      781 aacgctgctg atccagaatg tattgatccc cctcagcttt ctcaccgcag gcgggtggat
      841 gttccgccgc cgggaaaagg ccggggcgga cttctggctt ttggccggtt tcctttgctg
      901 gtacgccgtc acccgtattc tgaacggctg ccattacctg caggatgaaa gcggcttcct
      961 gccaaggctt gcctgcttta ttctggcgat ctatccactg gctgcggaat ggaaagcgga
     1021 tcgccgggaa aaggcgtttc atttctgtct ggctctcttc acgggcgttc tgaccatact
     1081 ggcgtgggtc ttgctgtacg cggctattcg cggggagccg gtgcagcctt tttccttcgc
     1141 ccgggtcatc ggctttaacg atgtttacgg cgctcggctg aatctttggg atctgcaccc
     1201 caacgtgact gcggcgatct tcgtggccgg tttcggaatg agcctgtgcg gcgtctccct
     1261 cgcccggcgc tggcctgcca aagcggccta tgccgtggcc gccgtcagtt tgtttgcggc
     1321 ggccagcctc acggtcagcc gcacggccat gatcgccgcc tcgctggaag ccgccggttt
     1381 cgtctttctg ctgatcgccg ggcgactgcc ttcaaaaaga aaggcgctat gcgttgtgct
     1441 ggccctgctg ctggcctgcg gcacagccta cggagcctac aagggttatg aagccatcgt
     1501 cagcggcgtt gcgtcggcga gtcaggcttt gcagcacgcg gcagaaacga cgctccccac
     1561 gccccagccg gaactgccct ccgatgcgac ggaagacccc gcctcctctg cggctgattc
     1621 ggttcccgcc gcctcccaaa cagagggcgg ttcgcccgcc aactcggctg atgaggggat
     1681 ccttccccag aaccgggaac tgacgcagga tcttctcacc ctgaccgggc gtaccaacga
     1741 tatttacggc gcggcgctcc ccgttctcaa gcagcgcccc tctatcctgc tgaccggcag
     1801 tctggacggc gaaaccatcg gcatcgccac ccgcatggct gggcgaccgc tgtatcactg
     1861 gcacaactct ctgctgcaaa cgctgatgct cacgggcatc cccggttttc tggcggctgc
     1921 ggctttcctc gtgctggtgg tgatcgcagg cgtgcggatc ttcttctccg gcaaagcgcc
     1981 gctgtaccgg cagctgctgg tactgcctgt ggccgggctg tgcctgcagg ggatgctgga
     2041 aagctacctg ttctgtcagg tagaattgcc gttttacttc tttctggttc tttccggcta
     2101 tctgcttgcc tttgaccggg aaaaccgggt acgnnnnnnn nnnttctgcc gcggtttttt
     2161 cgtatttgca cccttttccc ccttcgtggt ataataaatt gttgcggctt atcgccggtt
     2221 gtcatcgtcc tcggtgctgt gcaccctttt ataaagagat ttggagtggt taaacctcat
     2281 ggcaaagaag tatgacgcca gcagtattca ggttctggaa gggctggacg cagtacggat
     2341 gcgtccgggc atgtatatcg gctccaccgg cagccggggt ctccaccata tgctgtggga
     2401 gatcgtggac aacgccatcg acgaggccat gggcggctat gcccatcatg tgaaggtcac
     2461 cctgtacaac gactgctccg ccagcgtgca ggacgacggg cggggtatgc ccgtggacat
     2521 ccaccccacc atgggcgtca gcggcaccga agtcatcttc accaagctgc acgcgggcgg
     2581 caagttcaac aatgaaaact acgcctattc cggcggtctg cacggcgtgg gcgcgtccgt
     2641 ggtcaacgcg ctgagccgtt ggaccaccgt ggacgtattc catgatttta agcattatca
     2701 agcccgcttc gaaagcgtga ccgacccggc cacgggcaag atcaccgccg gccacaccgt
     2761 gcagcccctg aagcagctgg gcaacacccg gcagcggggt tccttgatcc gctttctgcc
     2821 ggacgaccgt gtctttgaga ccattcattt caatcacgat accgtctgcc gccgcctgcg
     2881 ggaactggcc tatctgaaca agggactggc catcaccttt accgacgagc gcatcccgga
     2941 aaagcccgcg gtttctgccg aagtcgacgc ggacgcggcg gaggaaaatg ccgagaacgc
     3001 cgccagcgcg ccccagcgca ctcagacctt cctgtttgag ggcggcattc tggactatgt
     3061 gaaatacctg aacgaggata agacccccct ccacgacgag cccatctaca tcgagggcaa
     3121 gcgggaggac gtgatcttcc gggcggccat ccagtatacg gacagctaca cggagaatgt
     3181 gttctcctat gtcaacaaca tccccaccgg cgaaggcggc agccacgaaa cgggcttcaa
     3241 agccgcctat accaaggtga tgaatgattt tgcccgccgt ctgggggcgc tgaaggacaa
     3301 ggatcccaat ctggggggcg acgacttccg ggagggcatg accgccgtca tggtggccat
     3361 ggtcaaaaat ccccagttcg agggccagac caagggacgt ctgggcaata cggaagtccg
     3421 tccgctggtg gaagccatcg tgacccagca gctgaccgcc tattttgaaa acctgaaaaa
     3481 tcaggagctg tgccagaagc tggtggaaaa ggccgtccgc gccgcccatg tccgggaagc
     3541 cgcccggaag gcccgggacg tggcccgcac caagaacgcg ctggaagccg cgccgctggt
     3601 gggtaaactg gccagctgca ccggccgcaa gccggagctg aacgagctgt tcatcgtaga
     3661 gggcgacagc gcaggcggca gcgccaagca gggccgcgac cgccgttttc aggccatcct
     3721 gcctcttcgc ggcaagccgc tgaacgccga gaaaaagcgc atcgatcagg tgctcaacaa
     3781 cgaggagttc cgctccctca tcaccgcgct gggcaccaac atcgacgaga gtttcagcct
     3841 gaacgggctg aaataccata aagtcatcat cctcagcgat gccgatcagg acggcgcgca
     3901 tatccgggcc attctgctca ccttcttcta ccgttacatg cgggagctga tcaccgccgg
     3961 gcatgtgtat atcggaatgc cgcctctgta caaaattcag cgaggcaaca aggtgcagta
     4021 cgcctacgac gacgcggaac tggccaaact gaccaagggc agcgccaagg gctacaccct
     4081 gcagcgctac aagggtcttg gcgagatgaa ccccgagcag ctgtgggaga ccaccatgga
     4141 tcctgccaac cgcaagctga tgaaagtgac catcgaggac ggcgcacagg ccgagcggct
     4201 ggtcactatc ctcatgggcg acaaggtgga gccccgccgg gaatacatca gccagtatgc
     4261 tgacttcaac cgggaagaca gcttcaccac cctgaccgac agtctggaag aaggaggcaa
     4321 gcgctcgtga gccgcaaaga cgacaagacc ccggtcgtag aagaaaacat tctctccgtg
     4381 cccatggagg aggtcatgca tatgagcatg atcccctatg ccgagcacgt catcatggat
     4441 cgggccattc cccgggtgga ggacggcatg aagcccgtgc agcgccgcat cctctttacc
     4501 atgaacgaat tgggcatcac cccggacacg ccgtaccgca aatgcgcccg tatcgtgggc
     4561 gactgcttag gtaaatatnn nnnnnnnnaa atccgccacc tgcgtgggcg acgtgctggg
     4621 ccggtatcac ccccacggcg acgcctccgt gtacgacgcg ctggtgcgtc tggcgcagga
     4681 tttcagcatg cggggcacgc tggtggacgg ccacggcaac ttcggctccg tggacggcga
     4741 cagcgccgcc gccatgcgtt acaccgaggc gcgcatgacc ccgctggcca tgctcatgct
     4801 caaggacatc gacaaggaca ccgtgccctt ccggttgaat ttcgatgata ccttgaagga
     4861 gccggacatg ctgcccgcca gcttccccaa tctgctggtg aacggcgcgg gcggcatcgc
     4921 cgtgggtctg gccaccaata ttccgcccca caatctgcgg gagagcatca acgcggtcat
     4981 tgcccagatc gacaacccgg atatcaccgt ggacgagctg atgcagatcc tccccgcccc
     5041 ggactttccc accggcggca tcctgctcnn nnnnnnnnga cccacattga ggacggcccg
     5101 gcaggccgga agctgatcgt cattaccgaa atcccctatc aggtcaacaa atccgccatg
     5161 ctggagaaga ttctccgatt gagcgaggaa aagaaggcgg cgctgggctg catctacgac
     5221 atccgggacg agagcgaccg cacaggcatg cgggccgtga tcgagctgcg cagggacgcg
     5281 gacgtggaca aggtgctggc ctatctgttc aagtacagcg atatgcaggt caccttcggc
     5341 gtgaacatgg tggccattgc cgacggcaag ccccggctat tgagcctgcg ccggatgatc
     5401 cgctactaca tccgccaccg gaagaacgtc atcaccgccc gcacccagta cgagctggac
     5461 aaggccaagg cacgtgccca cattttggag ggcctgatga tcgccgtgga caatctggac
     5521 gaggttatcc agctcatccg caaaagcgag aaccccaagg cggccaaggc gggcctgatg
     5581 gagcgcttcg gcctgacgga aattcaggcg caggccattc tggacatgcg tctccagcgc
     5641 ctcaccgggc tggaaattct ggccctgcgc aaggaatacg ccgagcttct gaaaaccatt
     5701 gccgaactgg aagccattct gaaggacgaa aagaagctca tgaacgtcat caagaaggag
     5761 cttcgggccg tggccgatca gttcggcgac gaccgccgca ccaccattga ggaagtccat
     5821 aacgtggtgg aggcgctggt gaaggaagaa aaggtggcgg aggaagcgct ggtcaccttc
     5881 tcccgggaag ggtatctgcg ccgcagctgg cccaaggcca aaaaagcggc ccagcctgag
     5941 gacggtcagc cggacgatcc gcccgtgtat tcctttgaga cggacacgga tcacaccctg
     6001 ttcttcttta ccaatatcgg caactgctat cagctgaatg tgggcgcgct gccggagatg
     6061 aacaagccca aggatcgcgg ctctctgctc agcggcgtgc tggccgggct ggaaaacggc
     6121 gaagaacccg tctatctgnn nnnnnnnngc taaagcgttc cgccgccagc gagtacgccg
     6181 tgaagcgttc caagttcggc gcgctgaacc tgcgggaggg cgacagtctc cacgcggtgc
     6241 tttcgctgga cgtttcctcc gacgtgctga tgatttccga aaccggcatg tgcatccgtt
     6301 tccatgccga tcaggtgccc gccatgggcc gggtctccgg cggcgtgaag ggcatgaccc
     6361 tcgacctgag cgaccgcatt ctctgggcgg gccagcccgc cgcggcggat cagctgctgc
     6421 tctttaccga gcggggcttc ggcaagcggg tgccctacac cgattacgag ccgcaggccc
     6481 ggggcggcaa gggcgtgaag accttctatt accagaagag cggctccaat ggcacccgta
     6541 tcgccggggc ggcgctgatc accgctcccg ggcagaccgt ggtcatccac cagaagacca
     6601 gtcagcccac ccagctttcc gccgacgagg tcattctcca atccaagcag gatcgcggaa
     6661 tgccctacgt catggccctg atggacgaca ccgttaccgg cgtatccctg gcctgatccg
     6721 attgcccctt gtccagccct caaaaccgtg ctacactatc cctgttaata tcaacaaact
     6781 ggaggtattg accatggaac gacccaacgc gtggaaacaa tatgacgaag catccctgaa
     6841 cgagctgaag gagatcagcc aaaaataccg ggtatttctg gacaacggca aaaccgagcg
     6901 cgaatgcgtg accgaaaccg tcgctgccgc caaagctgcc ggttacatcg acctgaacga
     6961 tgccatcaag gcgggcaagg ccctcaagcc cggcgacaag gtctatgtga actgcatggg
     7021 caaggccgtt atgctcttca atctgggaga agctcccctg accgaaggcg tcaacatcgt
     7081 gggcgcgcac attgactcgc cccgcattga cttcaagcag aaccccttct acgaggacac
     7141 cgatctggcc tatgccgaca cccactatta cggcggcatc aaaaagtacc agtgggtggc
     7201 tcgtcccatg gccctgcacg gcgtggtggc caagaaggat ggcacgctgg tgaacgtggt
     7261 catcggcgag gacgcggacg acccggtggt gggcatcagc gacattctga tccatcttgc
     7321 gggcgaacag atgggcaaga aggccagcga ggccgttacc ggcgaggcgc tggacgtgct
     7381 gctctgcggc caacagctgg caggcgagga aaaggnnnnn nnnnncgtca aggccatgct
     7441 cctgtccatc ctgaaggaaa agtacgatct ggaagaagaa gacatgctct ccgccgagat
     7501 cgaagtggtg cctgccgaca agtgccgcga cttcggtctg gatcggagca tgctgctggg
     7561 ctacggtcac gacgaccgtg tttgctcctt tgcctcgctg gaagccatcc tccgcctgcc
     7621 cgccgtgccg aagcacacct gctgctgtct gcttgcggac aaggaagaaa tcggcagtgt
     7681 aggcgctacg ggtatgcggg ctcactattt cgaaaacgcc atggcggagc tgctggcgct
     7741 gacctctgag cagtacagcg accttatgct gcgccgggcg ctggccgcct gccggatgct
     7801 gagctgcgac gtgagcgcgg gctacgatcc cctctatgcc agctgttttg aaaagaagaa
     7861 tgccgcctat ctgggccggg gcgtctgcta caacaagtac accggctccc ggggcaagtc
     7921 cggctccaac gacgccaacg cggagttcct gggccatctg cgcaagatca tggacgacaa
     7981 ccacgtggcc tggcagactg ccgagctggg caaggtagac gtgggcggcg gcggaaccat
     8041 cgcctacatc tgcgccctct acggcatgga agtggtggac agcggcgttc ccgttctgag
     8101 tatgcacgcc ccgctggaga tcatcagcaa ggccgatctt tacgaagccg tcaaagccta
     8161 ccacgccttc atgcaggaag cctgacaagg ggcagcaggg ggtaaccccc tgcaccccac
     8221 tccgcgccca tagggcgcgg ggcgctcgtg ggaaagaaaa ttggctgcgg
//
LOCUS       DAWAEA010000017         8119 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_1452_length_8119_cov_2.528646, whole genome shotgun sequence.
ACCESSION   DAWAEA010000017 DAWAEA010000000
VERSION     DAWAEA010000017.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 8119)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8119)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8119
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1452_length_8119_cov_2.528646"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1526..1535
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7191..7200
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1581 a         2416 c         2455 g         1647 t
ORIGIN      
        1 aaagtactgc cccccctcgg ggcggcagcc ccaccgtccg cgccggagtc ggcgcgtcaa
       61 gccctcggcg tgattatgcg cccgcagacg cgctgtcggc tttccgttca ctgccccctt
      121 cgggccgcct gaagaaaacg aacgccccat agggcggcaa gccttgctcc cgcgccggag
      181 tcggcgcgtc aagcacccgg cagggggaaa tcctacggca aaatggccgc gcctgatgca
      241 ggcatccccc acctctgcgt cgaacccttt cttctatcag ggcttctttc caaaagaacc
      301 ctccccctct ctccctcttc tctttaatcg gaaggagcta acgaaatgca gttcttagca
      361 accatggaaa aactggtgct gcgctactat aacttctttc tggaaggcgt gcgcaatacc
      421 ctcattatcg cattcttcgc tgtcctgctg ggcaccctgc tgggtacgct gatggccatg
      481 gcccgcatga gccgcattaa gcccctgaag tggatcgcta ccgcctatat cgagttcttt
      541 cgcggaacgc ccctgatggt gcagctgatg ttcatcttct atggcctgcc catgatcggc
      601 gttaccttcc ccagcgtcag ctttattcct gatttcgatc gcttcgcggc gggtatcgtg
      661 gccatgagcc tgaacagctg cgcctatgtg gcggagatca tccgctccgg cattcaggcc
      721 gtggataagg gccagatgga ggcggcccgc agtctgggct tccatcacgg cgaggctatg
      781 aagttggtca tcctgccaca ggcggtgaag aatatccttc ccgcgctggg caacgagttt
      841 gtgaccatca tcaaggagtc ctccatcgtg tcggtcatcg gtatcgccga tttgatgtac
      901 cgggccaagg gcgttatcgc caagacgtat aacagtctgg aatgtctggc gatcgccgct
      961 atcatgtact ttatcctcac cttcattggc gggcggctga tcgccctgat ggaaaggaag
     1021 atggctcatg gcagaaaata aaatgattga agtccgccac ctgtccaagc gcttcggcaa
     1081 gctgacggtt ctcaatgata tcagcacctc tttcgacgca aaggaagtgg tcagcatcat
     1141 cggcccctcc ggcggcggca agagcacctt tctgcgttgc ctgaacctgc tggaaacgcc
     1201 ttccgatggg cagatcattt ttgacggcgt ggatatctgc gataaaaaga cggatatcaa
     1261 cgtccaccgg cagaagatgg gcatggtgtt ccagcagttc aacctgttcg agaatatgaa
     1321 cgtgatgaag aacctgaccg tggcccccat gcgcattcat aaaatgcctc aggccgaggc
     1381 ggaagccaag gcggaagagc tggtgcgccg ggtggggctg gcggataagc tgacggccta
     1441 tcccagccag ctttccggcg gtcagaagca gcggatcgcc attgtccgtg ctctgatgat
     1501 gaacccggag gtcatgcttt tcgatnnnnn nnnnngccca caagcgctct ggatcccgaa
     1561 atgatcggcg aggtgctatc ggttatgaaa gcgctggccg aggagggtat gaccatgatc
     1621 gtggtcactc atgaaatgcg ctttgcccgg gaggtcagta cgcggacgct gttcctggac
     1681 ggcggccgca tcgaggagga taagccctcc aaggagctgt tcagccaccc ggaaagtccc
     1741 cggctcatca gctttttgga aaaggtgctg taagtcatca aaaaacagga tggtagaaaa
     1801 gaccgtcctg ttttttgatg ctgttcattt ttcttaatcc ccttttcccg gcgcacggga
     1861 agtgctataa tgcggcgtat tccacccgta ccaaggaggg atttcccatg acctacaagc
     1921 acaccgaacg cgcctgcttc accggatata ttgtgcaggc ggtggtgaat aacttcgttc
     1981 ccctgctgtt tttgaccttt cagaaacagt tcggcattcc gttgtcgcag gtgacgctgc
     2041 ttattacgct gaattttctt ctgcagctgg ctgtggacgg cgcgtccgtg ttcttcattg
     2101 atcggatcgg ctatcggggt tcggcgctgc tggcgcatgg cttcgcggcg ctggggctga
     2161 tctttttggc ggtgctgccg tcggtcctgc ccagcgcttt tgcgggcctg ctgatctccg
     2221 tcctgttcta tgctgttggc ggcggcctgc tggaggtcat tatcagcccc attgtggagg
     2281 cctgcccatc cgagcataaa gccaagacca tgagtctgct ccattccttc tattgctggg
     2341 gcagcgcggg cgtggtggtg ttgtccaccc tgtttctggc ggtgttcggc agcggcagct
     2401 ggcgggtgct ggcgctgatc tgggcgctgc tgcccgtgac gaacggcatt ttcttcctga
     2461 aggtgcccct tgcgccgctg gtggaggacg gagaaaaggg gatgtcagtg ggcgaactgt
     2521 tccgctccgg catgttctgg gtcatcatgc tgatgatggt ctgcgccggg gccagcgaac
     2581 aggcggtgag ccagtgggcc tccaccttcg cggagaaggg gctgggtgtc agtaaggcgc
     2641 tgggcgacct gaccggcccc acggtgttct ccctgctgat gggcctgagc cgtctgatct
     2701 acggcaaacg gggcgacaaa atggacttga ccaagaccat gctgggctgc tgcggcctgt
     2761 gcgtagcggc gtacctgctc atcggcctga cgcagagtcc gctgacgggc ctgatcggcg
     2821 taggcctgtg cggtttctcc gtgggcattt tctggccggg cacgttcagc acggcgtcgg
     2881 cctccatgca ccggggcggt acggctctgt ttgcgctgat ggcgctggcg ggtgatctgg
     2941 gctgctccgc cgggccgacg gtggcgggcg cggtagccag cgccttgggc gataacctgc
     3001 gggcgggcat cctgtgcgga ttgggcttcc ccctgctgat gatcgtcggc gtactgctga
     3061 tgaagcgcat ggcggatcgg gccaagccgc aggcgtaaaa tttttatgga aaaaaccggg
     3121 tacagaggat agtttttact ctgcacccgg ttttttgaat tgcctgaaat ggatggccgg
     3181 tgtatacggc ccgccccgct ggcgccttat tcataaatat cggcgtgccg ggtactgccg
     3241 cgcttttact cagggctgcc agtcgctaag taccttcggc agttgtgccc actgggcgat
     3301 gatgaacttt tcaaatttca tttgaaaagt tcagtctgtc aaaggcagac cagctttccc
     3361 tagggacaaa ttaatattcg ggggtgcgga gcgggtttgc cgctgtcctc gccctttggc
     3421 gcgttccctt atttttgcgc gcctgtttgt gccattcgct ttcattatag gggaatcgaa
     3481 tgggaaggga cgttcttccc tgccgagggt cgggcaggtt cagaccttaa ggatatagcc
     3541 ttccttccgg ggcttgggct ggggagctgg ggcggcgccg tagcccaacg cgatggagcc
     3601 tacgccccgc agggtctccg gcagacccca tttccgcagc agcgccttgc ccgcttcgct
     3661 gtcgaaaatt tcccgctccc gatgcaccca cacggtggaa agccccagcg catgagcggc
     3721 cagcatcatg ttttccagcg cacagctggc gtcctccacc cacgggccga tggccgggtc
     3781 tgccagcgcc agcacgatca ccggcgcgcc gtagtaggga tccttgtccg tgcccatgac
     3841 ggcggcgttc agcttggaaa tttgttcccg atatttggga ctggtcacgg cgatgagggt
     3901 gggcgactgc ctgcccatgg ccgagggcgc gtaagttccg gcctccagca cctggtccag
     3961 cagttcttcc ggcacggggt cggccttgta ggcccggacg ctccggcggg tcttcaacaa
     4021 attcagcatt tcatgggaag cagtatccat gtggaaaact cctttcaaaa agcaatgctt
     4081 gtggaaaacc aaagggggat taccggctga cgcgcagctc ctgtcccggt tccagcggga
     4141 tgcgccgacc ctgccaggtc agcgcgccgc cgggacgatc ggtcaggacg cgcagctccc
     4201 gctcgtccca gcacagttgt acctgtccgc cgtccggcag gggaatgacg gtatccagcg
     4261 ccgatagccc ggagggtcgg ggctccagcg caaaggccct gcagccggga gcctcggccc
     4321 ggaaaccggc tacataccgg ccgatgaggt cgatggggcc cgcgccccac gcgtggcaca
     4381 ggcttttgcc gaagagatcg ccgtacatgg cgtagtggcc ggggaaggtc tgccgggggt
     4441 cgtattcctc ccagatggtg gtggcgccca gcgccagcat cccgccccag tagtcccgca
     4501 cctgatccag cattttttcc acccggccca gcttgcagtt ggcccaaagc tcgaaaaatt
     4561 tgaagtaggg cgtggtgatg gccggaacgc cgtcgttgtc cagcacgttt cggacgatgg
     4621 aagcccgctt tgattcgtcc gcgaaatcat acaggatggc gaaaatattg gcgtgacggg
     4681 tcacgttccg ccgcccggag gcgtaggtgt cgatataagc gcccttttcc tcgtcgtaga
     4741 aaaacttgtc cagctttttt gacagccttt cggcatgggc tgcgtaccgg ctgccgtcct
     4801 gcccagccag cgaagcgcag tctgccgccg cttccagcgc ccggatgaac agcatctgct
     4861 cggcacaaag cgggccgtcc ttgtccatgt cggcccagtc cataaagacc cagtcttcct
     4921 cccggccaac gaggaaccct tcctcattgg tgcgttccag acagaagtcc gtcagactcc
     4981 gcatcctcgg ccagatggcc cggacaaagt ccagatcgcc gtaggtctgg tagtcctccc
     5041 ggatggacag gagccagtag agagaataat ccacaatgga attcagatgc tgcagcagag
     5101 gctccttgcc ccgcagagcc cacatggtgc ggtggcaaat gtccgcgtcc atgtacagaa
     5161 accggctgac aaagtagctc tgatacgcgt cgcccgacca gacccaacgg tcccgcttga
     5221 tgccgtccag aaaaaattcc cggctgttga gttcaaaggt ataggccgcc gtgttccaaa
     5281 tatctgtcag cagccggtcg gagcaggaaa agcgcccccg gcgcttcagc ggcgtgcgtt
     5341 cgtattccac ccgcaggcaa agcttcgcct ggccgggaac gaaaacatag cggaacgccc
     5401 gggcagggaa ctggatcgaa gccgttccag cgggcacggt ctcgatcagc ggacagtcgg
     5461 atacggcccg cgcttcggtc tccgattcac cgaagcagac cgtcagctcc ggctggttct
     5521 ccatttccag aaagacccgg gcaaaggtct cccggccaaa gtcgtacagc gttccccgct
     5581 cgtcctgaat gacttcgacg ggatggagtt tctcatagat gaagggaaag acctccgggt
     5641 cctgctccgg cagggtaaat agggggctga aacccacggg tagcttttcc ccggcaaagg
     5701 gctgggccgt ccagccctcc gtgccggaca ggcttccttt ttccacatac aggcagggca
     5761 gcccgtcctc ccggaacact tccaccacga tctcatgcgt tccggcggac agggggattt
     5821 ccttcccgaa ggggtacagc ttaccgtcca cgcctacgtt gcccttgccc cgggcaagga
     5881 cggtcagccc ttccggggcg gtcagctctg cgcggcggaa aaaacggacg ctgtgccagc
     5941 agtcgtacac cggccagatc gccagccggt agtaatcccg ctcctgacgg cgggcgtgaa
     6001 gtttcagaga gtgacggagc tcgaagtctc cgggatacca gatccagcag ggcgtatgtt
     6061 ccataggcgc gttccccttt ttttgacgtt ctcctatgtt ttctccgaaa aaaggcgaat
     6121 ttccttgcgg aaaaagaaat tccctctccg atgaaaactc ggagagggaa ggaattacgg
     6181 tcgcggcgtg gagcgcacgc acagataact caagggcagg ctgaccaacg cggccaatcc
     6241 ctgtatcagc cgccaatggt cggtgaagaa agcccatgcc ttctggatcc agttgaccca
     6301 ttgcaggctt tgaatgaaaa tttcgccctc actgccgtac tgcaccagat caatggcctg
     6361 acggaacacg ggagccagcg tcagcaccca cagaagcagc ggccccagaa tgaccacggc
     6421 cagcgtgacc catttattcc gccgcaggca gcaggcaaac aggtacatca ggcacaacac
     6481 ttccagacag tccaacagaa gccggggaaa atcccccatc gtctgccgga aggactgggc
     6541 cagataggcc tccggcagga aggcgaaatc atagccgcac aggatgctga aatgttcatt
     6601 gtgtcggctg agagccagaa tgaggtagtt gttggcgagg cttagcagca gcgtacccag
     6661 cacaaacccg accatgcaca gggccagact gacgatctgg ctcagccata cggtgaaccg
     6721 ggacgtgccg aagcggcaca ggaacgtagc gccgcttttg gccagcatac agctcagcac
     6781 cagcatcaga aacagggtca gcgcccagtt ggctgaaacg ccggtgccct cgcaggtgga
     6841 aaagcccgtc agccccatga tcagggcgat gatatccgaa aggaggatca gcgccagcag
     6901 ccccagtgac agcttgccca gccgcagcag ggaatagcgc agcgcgccct tcatccggga
     6961 ggtgcgatag accgaaagtt tttcagccat gccgttccgc ctcctttgct tctgtcagga
     7021 atacgaacaa ccgctgcagg ctcatgggcg cggtctggat ggacgggtcc gccgtcagac
     7081 gctcccggtc ggcagggctg ttcagtcgaa ggtgccgcac cagcgaaccg gccatttctt
     7141 cctcccgcag cacggtcagc ccttccgtag cggcccgtac cgcatctggc nnnnnnnnnn
     7201 gatcagcacc gcggaattca gcacccgggc cacttcctcg atcagatggg tactgaggag
     7261 gaaggttcgg ccggacgcgc cgtccgggga gtgggtctcc tgccagagca ggtcgtagaa
     7321 acgttcccgc atgaccgcgt ccagccccag actgggctcg tcgaacacgg tcaggggcgc
     7381 gccggaggcc agccccacgc tcagcagcag ggcggtctgc atcccccggc tgaagccctt
     7441 caggcgtttc cgttccggca gctcaaaccg gctgagaagc tcctcggcgc ggctctggtc
     7501 ccaatgaggg aacaggccgc cgcacacgta aaacagttcc ttgatattgc tcagtttgcc
     7561 aaagtcaggg gtatcgccga tgaggcacag ccgggaaagg ctgctttccg gccggacagg
     7621 ctgcccgaaa agccggactt ccccctcgtt ggcccgaagc tggccggaga gaatacgcag
     7681 cagggtactt ttccctgcgc cgtttcggcc cagcagaccc acggtctggc cgggggcgac
     7741 ggagaaggta aggccgttca gcgcggtcat actgccgaag cggcgggtga cgttctgcac
     7801 ttccagtgcg gtagtggata tcatgggaaa ctcctttctg aaaaaggctg ggtggggctt
     7861 ttttcaaagg gttttggagg atgaaaaggg ggatatttgc tatgcggagc aaagccgctg
     7921 ttatggggaa tggcgtgttg attatttgtc ccgaggaaag ccaccagagt gtccgcaggc
     7981 gcatatcctt gctttatgga tacgccgact ccccgaaggg gtgcaggggg cgagcggaaa
     8041 gccccctgcc tgcatcgata ggcgacaact ttgccgaaag caattcccct tggtggggat
     8101 tgacacgccg actccggcg
//
LOCUS       DAWAEA010000018         8118 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_1453_length_8118_cov_2.264790, whole genome shotgun sequence.
ACCESSION   DAWAEA010000018 DAWAEA010000000
VERSION     DAWAEA010000018.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 8118)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8118)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8118
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1453_length_8118_cov_2.264790"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    697..706
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4845..4854
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7172..7181
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1615 a         2544 c         2383 g         1546 t
ORIGIN      
        1 tttcgctgct ctgcgtgcca tcgccctcga tatagctttc gtcgctgtgg gtgcagtaaa
       61 gggcgatctt ccgggcgacg gcgctgacgg gcgttgcgtc cgccacctga tccagccagc
      121 ccacgtcagg catatccgcg tcgccgatca gctccgtccg ggcggtatgg gcggattcgt
      181 ccacttccgt gacccggtag tgcttgttgt ccccggaaat atactcgtcg cccttttcac
      241 actggccctg atagcgggtc agcaccttgc cctttggcga aagcagcgtg tagatttccg
      301 gctcctccgc ccggctcagg cccggcaggc agcagcacag cgccgccagt aaaataaccg
      361 caatcgcttt cttcatggtt catttctcct tgtctcgcac gggattgcgc acggctgaat
      421 gcacgggctt ttcactgccc cggacaattc gctcggcaat ctcgccgatc agctccgcca
      481 gaatcacgcc gatcaggccg ctgattaccg ccgcatcgaa tacgcccgcg ccgcccagca
      541 ccagcgtttg ggaaattccc cgggcgctgt tgacgatggc tacaatgata tcggcaaaca
      601 gcacccccat gaccccgcag atgaacgcgc cccggcggga gcggcccagc acgtaggcga
      661 tcagcccggc gatgatgccg ttcaggtaca tgggatnnnn nnnnnncaga taggccgaaa
      721 cggcaaagac ggccccaccg gtgacgagcg cgcccacgat ggcccgaagc cgctccttcc
      781 acgtgcccgc ccggatcagc aggtagacgc acacgcccgc cggaatcagc gccccgccga
      841 tgctcaactc cactgtgccc agcttcaggg tgggaatcag cgtgccgaaa aacatcagcg
      901 cgatcaacag aagcgccgcc cggtcgctta agtacatctt atccagcacc cgctgggcca
      961 cgccgaagaa caccagaatg gcggcggcgg tcagcagaac cattcctacg gacataaaaa
     1021 ccccatcctt tcgaaaaatg tttcgtaaag atggggttac tttgcccgaa gccgggcggc
     1081 atcatacatt tttgcccatg ggagagctgt ttacgccccg gcgttctcct caaccagcgc
     1141 catttccggc actgacacgg tgaaacgcgc gccgcccacg tcaaaggtca gcctttcggg
     1201 aacgtgccgt ccgggttttc ctgcttctcg taccacgtgg agtcctctcc gcagggcgta
     1261 tcattgccgt cagggtaaat tttcagatac tgccacacct gttcccacag gtcgttttcc
     1321 gtttcgcctt cctccatagg cagggacaga cggaagctga cggtttggaa gctgacgctc
     1381 agttcttcca gcgtcagggg gcctttttcc gtggggacgg tcaaattcag gccggtttgg
     1441 ctcacagcgt tttccggtac ctgtgtggtg aaggaaattt tcaccacgtc catcaggtca
     1501 tatgccccgg aaatggtcag gctttcgaag ggctccatgc cgtaccattc ttcctccgtc
     1561 atgccgtccg ggatggggat ctggctgtcc cactccgcca gcgaggtatc gttggtcacg
     1621 aggatcttgt gttcccggaa ggcctgtgca aaatcgtccg tcttttgctg catttcttcc
     1681 tcggattccg tgccgtcaaa gaccacatcg gcgtattcca cctcaaagtt gggcttgaac
     1741 accttcatgg tcagcgtcca gcggacctcg cccgcagggc ggatcagatt gcagtcatcg
     1801 tccagcagca ccgcatccag cccgaagccg tagccctgcg ccgccagatc gtggccgggc
     1861 aggaaggggt acacgaagcc gctcatgaag tcgcccgcac cgaagctttc cacatccgcc
     1921 acataggtct ggccgtccac ctccgccgtc agtcgtggat acagatacag gtgcatatcc
     1981 gggtccttgg tggtaaaatt gaaggccatg gtgacggtct cgccgtcgta gagcacgtcg
     2041 ttggcggtca tggtcatgta ggcgttttcg taggtctcgt caatatcgac gatgctgttc
     2101 tgcagttcct ccgtcatttg ccccgcgccg ctgtaatcgg ccacggagta gcggctcacc
     2161 accgcatagg ccaccgcgcc cagcgccagc aggacaaggg tcagtaccag cgccagagag
     2221 acttttcgtt tcataggctg tttcctttct cggatccggg agagaacggc ccgctgctgc
     2281 gcttccggca gagagtccgg cagacagttc cgcaaggctt cccgaagctg cttttccgtc
     2341 atggaacatc ctctccttcc agagtcagtc gaaggttctt tttggctttg tccattttcc
     2401 ggtagaccgc cgagcggctc aggccgaggc attcggcgat ttcttcgccg ctcaggttct
     2461 gccagaatcg aaggaggacg atctgccgct gctcccgggg cagggcgttc acggcctcca
     2521 tcacttccct ttgggaagtc tccggggctg ccagcaggta ttgaccgcga tgcgggccag
     2581 ccaggcgcgc cggttctgaa gattcggcca gtcggcccga tggctccagg ccttgacgaa
     2641 ggtctcctga gccgcgtcct gcgccagatg accgtctttc agggtgacgg cgcacaggcc
     2701 cagcaccagc ccgccccatt cgtccatcat gcgggtcagt tccgcttctt cctcgtccct
     2761 gccaggtacc atggcatgga acatccgtat cccctccttc tttcgtgggg ttcgctttgt
     2821 acactcgtaa gacggaagtc gagtctgttt tttcccgttt ttcgaaaagt tttcttcgtt
     2881 tccgtccccg cggttgaaaa agccgcgttc ctgtggtatt ctgacggcgg ttttccccat
     2941 tcatccgcca tgggaggttt ataagatgag acatcatatt ttagtcaaat ggaaggaagg
     3001 gtgcaagccc ggcctggaac ccattcggga gctgtttcag gagacgctgg ccatcccggg
     3061 cgttgagagc gtggagcttc atcccaacgt gatcgaccgt cccaaccgct acgatctgct
     3121 gatcctcctg tgcatggaaa aaggcgcgct ggaagcctac gacagcagcg aggcccatca
     3181 ccgctggaag gaagtttatg gcgggatgat cgagaaaaag gcgattttcg actgcgagta
     3241 aaagtttggt tcatggcatg cgaacgggag ccgatgcact caagcatcgg ctcccgcaga
     3301 gtgtcaaaaa agtggaggtg caggaggcac tgcctcctgc cggagttata ggggcagagc
     3361 ccctaaccct tgtgccgcag cactttcccc gcaaaccagc gtagcgctgt ttgcgggcaa
     3421 aggcggccaa ctttaggttt ttcgacagac tgcgggagcc gatgcactca agcatcggct
     3481 cccgttttct attctaagcc gtttcttcca tgcagcgccg gacttcttcc ccattcagcg
     3541 cttccttttc cagcagggct tcggtcagcc gccgccacgc ggcttcctgc tggcgcagca
     3601 gcgacgcggc cagctgccgg gccatggaca gccattgctg ctgggcggct tcccgctgcc
     3661 cctgcgaaaa cagatagggt tcatccccgg cgggcagcat tccccattcg cagaccatgc
     3721 gctggcacag cagcgccgcc ttttcaatat cgttggacgc gccgttggaa atttgctcat
     3781 aaccgcccaa gtacagggct tcggcctccc ggcccgccaa cgctaccgcc atgtggtgca
     3841 atagctcctg tctgcggtga aagagctttt ccggcggaat ggccatgctg tagcccgccg
     3901 cgcccttgcc ggtggggatg atggagaccc ggcggatctg gctttccggc agaagcaggg
     3961 ccgtcagcag ggcgtgaccc gcttcgtggg cggcggtcag ttcccgttcg gccttctgtt
     4021 ccccgtcgtt cgggcgctcc atgccaaaga acattcggtt cagcgcttca tccacatcct
     4081 gccgggcgat ttcctccgcg tcccgcttgg cagcgcagat ggcggcctcg ttcagcaggc
     4141 tctccagctt cgccccggag aaaagagcgg tctccgccgc cagcgaagtc agagaaacct
     4201 ccggggccag cggctttttc cgggcgtgaa cctccagaat tttctgtcgt tcccgcacgg
     4261 ttggtcgcgg ccagcaccac gatgccgtcg ttttcccgaa atccgctcat ttccgacaaa
     4321 agggcgttga gggtctgttc ccgctcgtcg ctgcggttgt cccgcttttt gcccaccgca
     4381 tcgatctcat caaagaagat caccgctttc ccggccttgc gggccttctg aaacagcgca
     4441 cgcacccggc tggctcccac gcctacgtac atttccacaa agtccgcgcc gttgaccgcg
     4501 aagaacggca cgcccgcttc accggccagc gcccgagcca acagcgtctt gcccgtgccc
     4561 ggcgcgccat aaagcagcac gccccgaggg atccttgcac cgaaacggct gtatttttcc
     4621 ggcgaagaga gaaagctgac cagatcgcgc agactttcca gcgcttcttc gttggcagcc
     4681 acatcggaaa agtgaacgtc agggattgtc ccctcgctgc tgtttttcac cccggagaca
     4741 aagctctttt ccctctgccg tctgcccagc agcagaaatg cgggcggcag cagccaaagc
     4801 agcattagtc cgccgcgaag gccttccccg ctccgggctg acagnnnnnn nnnncagcgc
     4861 ctgtatcggc ttatgtttcg aaacgcgcat accatcacct caggtcctca aacggcaaag
     4921 aagcatcata gcgccgccgt ttgagaaaaa tcgtgtcggg tgaaagtata caggaattcc
     4981 atacggcaag taaatggtac ttattgtccg cgtttccagc gtatcagacg ggacagaaag
     5041 cgcaaagtat cagcggatat ttttgaatgg tttgcaaacg gagggatgcg gcgtgaaaaa
     5101 gaagaagcag aaagccagac cggccgcgga ggtgcgggaa aaattacctc ctgccggaga
     5161 agaaactctc ccctccgaag aaacggagca gccgtctgtg gcaacgcgtc cgcccttccc
     5221 ggaggaagaa aaaagcggcg aacaagccga aaaatttcag gagctttcgg cagagccagc
     5281 cgaggaaccg gcgcaggcga actcccccgc cgaagaagca ggcccggaag ccgcccagcc
     5341 ccaatggacg gtcactgcga gggaacgccg gacggcgcgg cgggaagaga aggcccggca
     5401 gaaggaggcg catcgggcga agaagcaggc ggcagacgag ctgcttcacc gggagcacga
     5461 catcagcggc ccggcagttg ggcacgcccg gccgctatca gaccgggctg ccggtccagc
     5521 ggcatcccct acgggcggcg ctgatggtgc tgctggtgct gggcatagcc ggaggaacag
     5581 cgggcttctt tgtactggac gtgcccaact ggcagccgct ggacgtgagc cgcatcaccg
     5641 ccgcgcccca gacgggccgc atgtacgacg gcgaaggcca actcatcatg accatcaaag
     5701 gcagccagaa ccgggtagtg atcccctttg acgatattcc cgagcagacg cggaacgtat
     5761 ttctggcggc ggaagacctg cgcttttacc gtcaccacgg cgtggacatg gtgcggctct
     5821 tcggcgcgct ggcagccaac atccgggagg gcggctactc gcagggggcc agtaccatca
     5881 ccatgcagct gatccgtcag agccatctga gcacgaagaa gaccctttcc cgaaaggccg
     5941 aggaaatctg gctggcatgg caactggaac gccgcatgag caaggacgac attctggcca
     6001 tgtatctgaa ctacatctat ttcggcaacg gtgcctacgg cattcaggcg gcggctcaaa
     6061 cttatttcgg cgtggacgcg cgggatctga ccctgacgca ggcctgcgcg ctggcggcca
     6121 gcatcaaggc tcccagctat tacgcgcccc acgtcagcgc ccagcacaac cgttcccgac
     6181 gaaactatat tctcaacatc atgctggaag aaggcatgat cgaccagcag acccgggacg
     6241 aagcggcggc ggaaacgctg gtgctggcgg aggtgctgcc agagaaggtt ccctacggct
     6301 ggtacgccga tgcggtgctg gacgaagcgg agaccctttt gggcatgacc agtgaagaag
     6361 tgctttccgg tggtttgtct attgatacgg cgctgagccg cacccatcag gacctgctgg
     6421 acaagcagtt caccgccgat attttcccgg caaacgccag cgacggcacc aaggtgcagg
     6481 gcgcggcggc ctgtgtggac accaacactg gcgaggtgtt ggccatcgta ggcgggcgga
     6541 gctacgacgt gcgccggggc ttcaaccggg ccacccggct tcgccgtcag cccggttcgg
     6601 ccctgaagcc gctggcggtc tacgcccccg ccatcgaccg ctacggctac atgacctgca
     6661 gcgtcctcaa ggacgagccc accaatttca acggctacaa gccccgcaat tccagctaca
     6721 cctactacgg caacgtcagc atccgcacgg cgctgaaaaa cagcctgaac gtctccaccg
     6781 tggccctgct caaccagatc ggcgtgaatt ccgggcggga ctacatggcc agcgttggca
     6841 ttccgctgga cgaccgggac gcgaatctgg cgctggggct gggggccatg acctacggcg
     6901 tttcgccggt gcagctggcg gcggcctacg ccccctttgg caacggcggc acctacaacg
     6961 cgccccatct gatccgccgc atcaccgacg cgtcgggccg ggtgctctac cagaaccggg
     7021 acagcggctc ccgggtgctg aaggctacct cggcctatct gatgaccagc ctactgcaga
     7081 ccgtcaccgc ctccggcacc ggcgcgaagc tgtcaggcgc gggtacgccc gtggcgggca
     7141 agacgggcac ggtcaacatg actggcgggg gnnnnnnnnn ncatctggat ggcgtgctac
     7201 aacagtgaaa tttcctgtgc cttttggatg ggctttgacg aacccgatca gaagcacaag
     7261 ctgcagggct gggtcagcgg cggcgacaac accgccgcca tggcccggaa ctatttcaag
     7321 gcgctgtacc agaaccggga caagccgcaa ttcacccggc cgggcggcat tattgcgctg
     7381 gagatcgacc agcaggccat caaatggcga ggcgaaccca tgctggccaa cgatctgacc
     7441 ccaaaaacct accgttacac cgagtacttc gccgaggaca accatcccac caaaaaaagc
     7501 gacgtatgga cgccgccccg ggcgctgaac agcttcaccg tttcccacag cgaggcgggt
     7561 tatcccgtgc tgatccttca accggcggac acggctctct accgggtcca gcgggacgcg
     7621 gtgggcgaaa gttttattct aacggaacta tacggttccg ccggggagac gctttatttc
     7681 acggacatgc aggccaaagc gggcgtagtc tatacatacc gggtgatccc cgttcacgcg
     7741 gagctgctga acaacggcgt tttgctggag ggcgtacagt cggtgcaggt ggcgcaggcg
     7801 aatctttcca ccggcttctg ggacagtgtg aagagctttt tcaccggcgg cgagcggaag
     7861 gaagacggtg tggtggagag tctggcggag aacgagcggt cgattttctg gggaaactaa
     7921 gagagaggat ggtcaagacc cgtctttccc cgatgaagca tgacgcgccg acggagggtg
     7981 cagaagtcta gcttacagac ccatgagttt cccgcaagct tcggtgcccg aaggtttcca
     8041 aaggggaacg aagggaagcg ggtatccagt ggatacaacc gccgaaggcg gttagcgacc
     8101 cggagttcag gcaaacaa
//
LOCUS       DAWAEA010000019         7883 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_1507_length_7883_cov_1.867527, whole genome shotgun sequence.
ACCESSION   DAWAEA010000019 DAWAEA010000000
VERSION     DAWAEA010000019.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 7883)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7883)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7883
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1507_length_7883_cov_1.867527"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2860..2869
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3493..3514
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4859..4868
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7402..7602
                     /estimated_length=201
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1497 a         2367 c         2333 g         1443 t
ORIGIN      
        1 ttggcgagaa acccataggc ggcagccccc ccttttgcgc cgtagtcggc gcgtcaggcc
       61 cgctggcgag attttttaca gccaagctga tacggcggtg cttgacagcc cccttttgtt
      121 gccgactaca gtgattaaac gctctgcacc ccctatcaca aataacgtcg aaaggagccc
      181 ttcccgcaat gaaacatacc aacctgacca tgggcatcct cttcggcttc ttcctgctct
      241 gcgccctgtc catgggcaac cgggtctatt tcgtgctggc gaccatcatt ttcttgagct
      301 ggatcttcgc ctttatcagc gtgcggatgg ccgagaaaac cgtgcaggtg tccaatgccc
      361 tcagcggtca gaaggtcaac cgcggcgaag tggtcagtat ggagatcgcc gtcagccata
      421 agggcgtgct gcccatcgct cccatcgctt tgcatatgcg cgccaccagc aatacccccg
      481 caggcgtgat ctacctgacc catttgggca aacgccgcca gcgggtgacc caccgcttcg
      541 tggccgacca cgtgggcgct atgttccccg gcgtggaaag ctatgtggtc agcgatattt
      601 tcggcttttt caaaaaagaa catcagcccg atctggcggg tcaggagctg ctggtgctgc
      661 ccgtgccctt cgaggtggag ccgctgacct tcgccgccgg ggatatgggc gtggaaacca
      721 tgaagcgggc catggaggat ccctccagcc ccagcgattt ccgcaattat cagcagggcg
      781 acccgctgaa acgcatccac tggaagatga gcgcccggaa gcgggagctg atcgtccggc
      841 agtttgagga accggccctg cccgacgcac tggtgatgct ggatacctcc ccgcctcatc
      901 tgaacgaggg cgtaccgaag gaaaatgccg cctttctgca ggacgcgctg ctggaaaccg
      961 ccgcctccgt ggtggcctgc cagatccggc aggataatca ggtgcggctg ccgctggtgg
     1021 gcgaccggcc catggagtat tcctcccata tgggtctgcc ggtgctgttg gaagaactgg
     1081 cccgctgcac ctttaacgag acggaacgct ttgagcgggt gctgatgatg cagatggccg
     1141 agctgcgcaa gctgggcgcg gtggtcgtca tcaccacccg gctgacctcc gaggtggccg
     1201 acctgctgat ccgcatgaag cgcatgggcc ccaatgtacg cctgtattat gtcaccttcc
     1261 gcaccgaccg gcccgagcgg ctggcgctgg tgggacggct gcagcaggcg ggcgtggagg
     1321 tgaactacgt cacgcctcag cttttccctg ctgggagcgc tgctgatcgg ccagtttttc
     1381 ctgccgggcg gcggcttttt cccttcttcg gtggaggccg tcaaggccat ttcactgtac
     1441 tttaacggcg tgacgaccgc gccgctgctg ttctcctccc agatcgcgct gctgctggcc
     1501 gtggtcgctg ccgtgatcgg ttataccttc tccgcccggg gcgtggggta catccccgct
     1561 accgccatgg tggtgatggt gcttttcggc ctgtggagca tgggcaagca gggtttcctc
     1621 tggtacgccg cccccgcgct ggtggcgctg ctgctcctga tcgcccagag ttcccatgaa
     1681 aaaagcaatc tgtttcaggt gctgcccatg gcagcgatgc tggtggcgct ggggcttttg
     1741 atcgcccccg gccagaacgt ggtgctgggg ccgctgcatg aggcggccat gaatctgaag
     1801 cagaccatta ccgactacct gttctttacc gagccccgca acgtgttcac gctgggcagc
     1861 tacggctatt atcccatggg cagcggcaag ctgggcggcg aggccgagcc gaccgagtac
     1921 ccggtcatga cggtaaagac ctcccagaag accctgctgc gggctgtgac caaggacgaa
     1981 tacactggcc gcagctggcg ggacacctcc tccgggcggc ggtatctgta cgtcaacccc
     2041 cgttggagaa gcctccgtca ggaagttttt ctggaaaata tgcccgccga gacggtgctg
     2101 aaggccagca acctgctgga tcagaaggcc atctccattc agatgcagaa cagcgcggcc
     2161 tccacggttt tcacccccgc ctatctgcgc tccctgacca cctacggcag catggtgccc
     2221 tatttcaacg aggccagcga gctgtttatc acccgggatc tgatctccgg cgacaagtac
     2281 accgtctacg cgcctgtgat cgagggcggc gacgcttcgc tgggcgcgct cgtcaacgcc
     2341 gcgcccaaga acgaccccta ttacgcccag atcgccgcca agtataccgc gctgcccgga
     2401 catctggaag aacgggtcta tcaggatatg cgcagcatga tcgccgacgc ggccacgccc
     2461 tatgagcagg cctgcgccat tctgcggcat ttgcagcgct actaccgcta taccctcagc
     2521 ccggtcacgc ccccggaaaa tcaggacttc gtcacctact tcctgtacgt gggcaaggag
     2581 ggctactgca cctacttttc gtccgccatg acggtgctgt gccggatggc gggcctgccc
     2641 gcacggtatg tggagggctt tctggcccag cccgattcca gcggtttcgc ctatgtgacg
     2701 ggccagaacg cccacgcgtg gacggaggtc tatttcgagg gcttcggctg ggtgcccttc
     2761 gatcctaccc ctgcccagca gaacgtggat cagcagcccc cgcagaacga cccggagccg
     2821 gaacccacgc cgacccccag cccggaaccg ccgcaggacn nnnnnnnnna gccggatcag
     2881 cccacgccca gcccggaaaa cccggagcag agcaacgaac cggacgatac cgataccccg
     2941 gacgacgagc ccgaccagaa agacccgccc tctccgtggc tgtggctgct gctcctgttg
     3001 ctggcggccg tggccgcggt ctgcgtcagg attttcctgc ggatgccgga tcggatggcc
     3061 aaaaagcagc agaacgtatc ggatcagatc ttcctgtatg gcagcgccgc ctataccctg
     3121 atgaagctga cgggccgcac gccccggaag ggagaaacgc ctctgcgctt tgcccgccgc
     3181 atggataagc agcgcgcctt tgccgtgccg gtcacgcccc tgtggcgcat gatggccctg
     3241 agcaactaca gccgtctgga acccacggaa aaactggccg gtcaggcgcg ggatatcttc
     3301 aacgccctgt acaagcctca aagtctgtgg gtgaagctgc gctttatgct ggccgctggc
     3361 tttgggaaaa actgctaccg cagtctggat acggagctgg tgcatcagga gccggaaagc
     3421 cccttcggca agtacaagga cagtctgaag caggctgcac aggcccctaa gaaaaacaag
     3481 cagaaataaa agnnnnnnnn nnnnnnnnnn nnnnctgatc ggccacgatc gcccgtgaac
     3541 agcgcttcgc tggttcacgg gaaaaaactg gggtacaaag tttcgtggct ccgccgccag
     3601 caccggcacg gggcagcgta tcctgcaccg ccgatttttg gacaaaaaag acccgccgga
     3661 agtgatcctc ccggcgggcc atttttttcc attcagcggc aaaatttttc gatgtactga
     3721 tccagacact tctggatgac cttcttcgca tcctccgcgc cccgcacctc gtccaccatg
     3781 gtctcctttt gctccagatt tttgtagacg gtgaagaagt gggacatttc cgtgaagatg
     3841 tgggagggca gctcgctgac atcgtggtag ctgttgtagg tgggatcctt gaaggggatg
     3901 gcgatgatct tttcatccag cttgccctga tccagcatgg taatgacgcc gatgggatag
     3961 cagcgcacca gactcagggg ctggatcacc tcgctgcaaa gcaccagcac gtccagcggg
     4021 tcgttgtcgt ccgcataggt gcggggaatc aggccgtagt tggcgggata gtgggtggag
     4081 ctgtacagga tgcggtccag gatcagggaa cccgtttcct tgtccagctc gtattttttc
     4141 ttgctgccct tttcgatctc gatgacggcg ataaagtcct ccgccgtgat gcgggaaggg
     4201 gaaatgtcat gccagatatt gctcataatc gttgctcctt tcatgatgga aagcggacgg
     4261 aacgctatgc cagcgcgatc cagtaccgct ttgtgcgaac gccgtcgtgc tcgtatgtac
     4321 tgtcgaacac gccgccgttt ttcacaatga ctttttcact ggcggggttg tcctcgctgc
     4381 aggtgaccaa cacccggctc agccccagct gccgggcgtg ggggagaagc tgccggagca
     4441 tttccccggc gtagcccttg ccccgctcac tgggacgcac ggaatagccg atgtgtccgc
     4501 cccatgtgga gagcaccgga tggtcgatgg aatggcgcag gtcgatcatg cccacgaccc
     4561 ggcggtcctt cagccgcagg gccagatagg tgtcggaggg caccagcccc tcggggcagg
     4621 tctcgggtcg ggtcaggcgg cgaacccgat ccagccaacg ggctgcgtcc tcaaaatttt
     4681 ccagcccgga gcagccgtcc atgctgctgc ccgcgtccag catttcctgc cggtaggcgc
     4741 gaatctgaga tagaaacacc accgagggcc gcaccagctg cagggaatcc gggccttcgc
     4801 ccagattgct gagcttttcc accaggcgag cgccccgttg ggctgtccgg ggcagcacnn
     4861 nnnnnnnngg tcgtacagct taaaggaatt ggtcagggtc aggaaggtgc agatggtaat
     4921 ggagggcatc acattgggaa tggtgatctt ccacagcgtc tgccagccat tggcgccatc
     4981 gatgcgcgcc gcctccagca cggtatagag cacctccacg aaggtctttc gggcttcgct
     5041 gtccagcccg gccatccagc tgtgaagggc ttcgtcggtg atgcggcccg tcatgtccag
     5101 cccttccaga cagacgaaat gcccgccccg cacctgccac gtcatggcgt cgtgctgcag
     5161 gatgcccacg gcgtccgact ccaccacggt gtaaacggga tgatagtaca tcagcatccc
     5221 cacgacgctg ctctggggaa tatagctgtg gatgcggtcc ttgacggcct gatagccctc
     5281 ggcgtagagc tggggccgat acatgcctgg cccgtcgaag ctgtaggcgg cccggagccg
     5341 ctgttgcgcc tgctcccccg caaaagacgc ggcgtagacc gacagattgc cgcccttgct
     5401 gtggccggtc agctccagcg gcccctccgt ccgggcggca atatccgcca catatcgggc
     5461 ggcttcctcc tgcgcgggca ccaccatgaa ggacatgttc atatcctcca cccagcctgc
     5521 gatattcagg tcggtgcccc ggaagctgac gcagaccgcg ccgtcgtcca gatcgtagca
     5581 ggcggcgcag aactggatat cttgttcgcg gtcgatgtgg ttcacatagt cgtgcacctg
     5641 ccagccctga tagcggggca gaccggcgca gacctccgtc agtcggtagc gcttctgctg
     5701 aaagcggtcc agcttttccg gctccacata gtcccgcaga aaggcccagc attcccggac
     5761 ggtatggctt tccccgcgat ccagaaagcc ctcgaagggc atgtagacca gctgggtcaa
     5821 aatcaggctg tccagcgcgt cgaaggcgcg tttcgccagc cgctgcccgc cgatctgaag
     5881 cgcgtggtcc tgaatgttcg gcataccgtt cctcccgtcc gcgccgccgg tcaaagaggg
     5941 ggcgcatcgg cattttagca cggctgggcg gggattggca aggggatgaa cttaaaccgg
     6001 gtagcgccgg tactggccgg gcgtaaagcc ggtgtagcgg cggaacagct tgcagaaatg
     6061 ggcttcgtcg cagaaaccca gatgaaaggc aatatccgcc acactgaggg tgtcgtcggt
     6121 caacagctgg cgggccttat ccagccgcag ctgaagccag tactgcttgg gcgaaagctc
     6181 cacgcaggcc cggaaggctc ggtagaaggc ggcttcgctc atgccgcaca gctctgccag
     6241 ccgggggatg ctcaggccgc ccgcaaggtt gcggcggatg tgctccaacg cgggctgcag
     6301 ggccatgagc cgctgcccgg caaacccggg actctggcgc agcaggtaga tcaactcata
     6361 cagcttggcc tgcaaaagca gctgggagcc gtgaacgtcc cagtacaggc tggcaagctg
     6421 gcggaggagc agctcgcatt gcccggccag cgcagccggg ggcacgatgg gggaaaactc
     6481 gcccagccgt gcgggctgcc cggcttcgtc cagcgccaaa aacttgacgc acaggttggt
     6541 aacgattctg gggccgtggg gcaggttgac gtttggatgg gcgggattgt ccacctgcag
     6601 ggcgaagcgg ctgccccggg gcgtgtagaa aatgtcgccc tgccggggat ggaacaccgc
     6661 gccgccattc agccgcaggg taaaggcgca gccgctttcc tccggcagaa agaacagcag
     6721 cgcgtcctcc ggccgggggg actccagata gctgtaatgg ccccggtcgg cccaaaactg
     6781 gggcacggcg cttacaagat gcaggcgcag agcgtcaaaa tcttccgaaa gcggcatgat
     6841 cttctgcctc ccgactgatc gaaaaataca aaagaaaaga gaaacatacc ttgcgcggcc
     6901 aattcgggaa ccgtacaatt atttccgtaa aagtatagca tgaacgttgc aaaatcgaaa
     6961 gaatctgccg aaaaaataca agcaggggag gaaaaccggc ggcggaacgc cgggaacagg
     7021 aacgatggaa aaatactttg gcgtttgcta ttacccggaa ctgtacccca aagagaaaca
     7081 ggaagccttt atccgggagg acatcgagaa tatgcgccgg gcccggttca acgtggtgcg
     7141 catggcggaa ttctgctggt gcctgatgga accccgagag ggcgtgtacg attttgactg
     7201 gctggaggag atcgtcgacc ggctggggca aaacggcatt tacaccgtgt tgtgtacccc
     7261 caccgcctgt cagcccgtct ggatggcccg ccggtatcct gaaacactgt atgtggacaa
     7321 tctgggcgtg cagcgcagct acggcgggcg gcactatcac tgctacaact cccccatttt
     7381 tcgggatctt acggaaaaga tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaacaaggc cttcggctcc
     7621 ctgttctggg ggcagcgctt cgacggtttt gaacagatag agccgcccgc cgccggttcc
     7681 cgggaagacg cgaaaagcaa tctgggctgg cggggcacca atatgcccgc cctgcggctg
     7741 gaatttgagc gcttcgccag cgacagtctg gtggattact tccgtctgca gcgggacgca
     7801 ctgcgccgct tcagcgacaa gcccgtcacc cacaacacca cccatctggg cactaacgcc
     7861 atcgactatt tcaagctggc ccg
//
LOCUS       DAWAEA010000020         7779 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_1530_length_7779_cov_2.247799, whole genome shotgun sequence.
ACCESSION   DAWAEA010000020 DAWAEA010000000
VERSION     DAWAEA010000020.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 7779)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7779)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7779
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1530_length_7779_cov_2.247799"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    478..600
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1464 a         2318 c         2474 g         1400 t
ORIGIN      
        1 cacccggaca gcctcgacaa gggcggcttt ctgcacggga cggttcatgt acatcaggga
       61 gtaaaagaag gtgacgttct caaaagaagc gtctgcaaag gcaagggacg ctgcgtccat
      121 cagcagcttc gggcaggaac agggcgcttc gtccagctcc tcctgacaat ggtcgatcat
      181 ggtgacggac gggccgtaca gccggccgat caccccttct ccgccgccgc ccacatccag
      241 aatggagtgt atgatagccg gggactatga ttttttttcg atgcaaagga gcagaacgat
      301 gcggatctac tctctgaacg gcgagcagtg gctcatgcgg caggcagggc aggaacagtg
      361 gcgcaaagcg aaagtgcccg gctcggtgta tgccgacctg atgcgggacg ggacccttcc
      421 cgaccccttt taccgggaaa acgagctgga ggcctttgcc ctgttggaaa acgatttnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 cgcgtatttg aagcggaaga aaccatgctg cagagccgaa aggtgctgct gcgctgcgag
      661 gggctggata cgctggccca tgtcaggctg aacggccagc ctgtgggata cgccgacaac
      721 atgcacatca cctgggagtg ggacgtgaag cccctgctgc tgcccgggga aaacgtgctg
      781 gagatccggt tcgattcgcc catccgctat gcgctcagcg agtatgaaaa gcgccccggc
      841 tggtgcagca cggacgcgat ccccggcttc cagcatatcc gtaaggccca ctgcatgttc
      901 ggctgggatt ggggcccccg tctgccggac gcgggaatct ggcggccgat cagtctggtg
      961 tgcgtgaacg aggtccggct ggaggacgcg ctgatcctgc aggagcatcg ggacggaaag
     1021 gtcttcctga aggtcacgcc ccatgtggaa tgcgagcccg gcgcggaatg gagctggtcg
     1081 gccgccctga ccacgccgga gggcgaacgc cgccccatgg acgaggacgg ccgcatcgtg
     1141 atcgaagacc cgcagctgtg gtggcccgcc ggttacggcg agcagcctct gtatcaggtg
     1201 tgcgttacgg ttgacgcgcc cgacggcccc cagcagtgga aacgccgcat cggcctgcgc
     1261 actctgtcca tctcccggga gaaggatcag tggggcgagg aattctgcca tatcgtcaac
     1321 ggggtgaagg tgttcgccat gggcggcgac tatatcccgg aggacaacct cctcagccgg
     1381 gtcacgccgc agcgcacccg ccgcctgctg gaggacgcga ggctggctca tttcaacacc
     1441 gtccgcatct ggggcggcgg atattatccc gacgatttct tttatgatat ctgcgacgag
     1501 ctgggcctgc tggtgtggca ggactttctc tacgcctgcg ccttttacga cctgaccccg
     1561 gcgttcgagg cctccatccg caaggagacg gagcagaacg tgcgccgcct gcgccatcac
     1621 ccgtcgctgg cccttctgtg cggcaataat gagatggaga tgttccaggc cgctgcgctg
     1681 aagctggata tgaactgcga gaaccacgag tttgccccgt ccgccatgca tcatcacgcg
     1741 gactatatca aaatgttcga gtccatcctc ccggaaattt gccgccggga agcgccccag
     1801 acgttctact ggcccgcgtc cccctccagc ggcggttctt ttgacgaccc caacggcgaa
     1861 aaccggggcg atgtgcatta ctgggacgtg tggcacggcg agaagccctt taccgagtat
     1921 cggaagttct tcttccgcta tacctccgag ttcggcttcc agtccttccc ctgtctgaag
     1981 accgtggaga gcttcaccct gccggaggac cggaacatct tctcccgggt catggagcgc
     2041 caccagcgca ataaggccgc caacggcaag attttgtcct atctgagcca gacctaccgc
     2101 tacccggaga gcttcgataa cctgctgtac acctcccagc ttttgcaggc ggacgcgatc
     2161 cgctgcggtg tggagcactg gcggcgcaat cggggccgct gcatgggcgc ggtgatctgg
     2221 cagctgaacg actgctggcc cgtggcttcc tggtcgtcca tcgactattt cggccgctgg
     2281 aaggcgctgc actacgcggc caggcgcttc ttcgccccgg tgatgatctc ctgtcaggaa
     2341 gaaggcgaag tgagccaaaa tccgcagatc aacgagttcc gcactgagcc tatccgggtc
     2401 agcgcccggc tgtgcgtcag caacgagacc cgggaggcgg tcagcggcgt ggtacgctgg
     2461 gccttgcgca ccccggatgg ggccgttgtt cggcagggcg aaaagacggt ggtcgtcccc
     2521 gccctgtcca gccggtggct ggaggaggaa gccttcccgg aggccagcgt gacggagcat
     2581 tacctgagct atgccttcgt cgtggagggc gaggccgtca gccggggcgc ggtgctgttc
     2641 tgcgcaccca agcatttcgc cttccgcaat cccgacctgc gggtctcggc gcaggacggc
     2701 gagatcacgg tggaagcgtc cgcttttgcc aaggccgtgt atgtggaaag caacgacccc
     2761 gacctgctgc tcagcgacaa cttcttcgat atggaccccg gcattcggaa gctgcaagtg
     2821 cttcggggaa gcgcggaggg gcttcgcgtc cgtagcgtat acgacatttg acagatgtgg
     2881 gggatcaggc cggggcgacc cgcttgcggc cctgtttccc tgcgctgcat tgattttttt
     2941 catgagaaag gcggatggaa ttgtctcgtt tttggatggg tctgctttta ataggcgtgc
     3001 tgctttgcgg cctttggttt ccggcgacgg cgcaggtgta tgaacagaaa atgcccgctg
     3061 actggcagga gaaggacgtt ctgcggataa cggccttcgc cgtgggcgag ggcgacgcgc
     3121 tgctgctgca atgcggcggc gaaagcatga tggtggacgg cggccccaag ccctttcggg
     3181 atcccatgaa ggcggccatg gagcagcggg gcatccgcca tttcaagtat ctgctgaaca
     3241 cccatagcca cgacgaccat atcgacggac tgtattacct gatgctgaac ggcttcaccg
     3301 ccgacgagta cctgcacccc tacaccgagt ggtgggcccg ggcggataag gagctgcatg
     3361 gacgcaccat caaactgacg gaaaagctgg gcattcccaa ccggcaggtg ggggaagggg
     3421 acacgctgac gctgggcggc gcggagctga agctgcaccg ctacaccaag gtgaacaaca
     3481 ccaatgccaa gtccctgctg accaaagtga ccttcggaca cgccacggcg cttctgtgcg
     3541 ccgatattac cggccgtgcc cagaagggtt tcctgaccca gcttgccccg gaagacctga
     3601 aggcggacgt gatcaagatt ccccatcacg gcatcacgcc ggtggagccg gattttctga
     3661 ccgccgtcag cccggagttc gctatcgtga ccaattacgc ggatgaaaaa aggctctcca
     3721 agctgatgta tcagttggag ggccgggaaa tcccgcacta tttcagcgga gagggaacgg
     3781 tggaactggc caccgacgga acggactggt acgtgtggca gacactgggc gaattttgat
     3841 tcagcccacg tccatgacca gcacctcggg acgctcactc atgtcccggg ccgtcagccg
     3901 caggaagggc acgcggggcg cgttcgcggc attgatctgc tgcaggaacg tctccacgcc
     3961 ggcaatgggc atgtccgggt cgaagtccgt tttgtaatgc atagggatgg tcagccgggg
     4021 acggatcagc tgacgcacct gcgcggcttc cgccgcgtcg atggtgtagt agccgccaac
     4081 gggcagcagc agcacgtcca cccggcccag ctcccgcacc tgcgcgggcg tgggaatgtg
     4141 ccccagatcg cctaagtggg cgatgcgcag tccttcgctt tccagcacga acatcaggtt
     4201 ttgtccccgg tgcgcgccgc catgatggtc gtggcagcag gggatgcccg tcagccgtac
     4261 gtcgggcgcg ggctgcacct ttccggccgt gtcgatcagc ttatattcgc cggtcaggct
     4321 gctgaccccg tcgtgatcgt ggtgatggtg gctgacgacg cacacgttcg cggcgatccg
     4381 gcgcttgggg taatcgtaga actccccata gggatcaaag gcgacaacgg tgcctttttc
     4441 ggtagtaacg gtgaagaagg aatgccccca gtattccagc agcatggctt acgctccttt
     4501 ctggttttgc agatggacgt tgagcagctg gccgcccagc gtgggcagcg gtttcccctg
     4561 ccgcagaagg gcggcgcagg tctcgtgaag ggatttgagt tctccgggtc gtaccgtcca
     4621 aagccacagg gcccggttca gctccatggg ctggacggcc agcggcgcgg ggtgcagcag
     4681 cagcagacgg cacagcgaat aggcggcgaa gtcttcctcc cgttttggaa aggcgtgaag
     4741 ttcaagggtc aaactgcccg ccatgtcctg ccagacagcg tccgcccggt caatgagcca
     4801 tagccccggc cgtttttccg gcaggagccg ccagcccaga cgggtcagcg cttcctcggc
     4861 cccggacagg tcgccgcccc ggcggggcag gtcgcacacc agcagcccct ccggcgtatc
     4921 gctgaagcgg agaaaacagc cgttagggct gaaaggctgc aaggcttcgt acagcgccgc
     4981 ttttgcatcc gtcagaaaga ccagctcatt catgggcttc gttttggcgc atcagccgaa
     5041 tctccatttc atgggccgcg gcgaactggc cgttcaggtc catctggtaa tggagcgaaa
     5101 tttccccgcc ttcttcatcc agccgcgttt tgagccgggt gcagaacagc gccagatcca
     5161 tgctgccgaa gggcgtgtca tactgcccct cgtagcgctg gccccggcgg aagatcatct
     5221 gggtggcgta gtccccgccg cgctccatca tcagtgtgtc atcctccagg gtgatggtga
     5281 tgggctgggg cggcagggct tcgtccagca cctcctcata ggtcagaacg gagcggccgt
     5341 cctccagcgt gagcctgccc gaggtcagca gggtaatggg ttcttcctcg tcgtcgcggc
     5401 gggcgacggc ctgcagggaa accagcacgg gaatcgtaag cgtactacca cctcattatg
     5461 gaaatccttg agaatgtact atatcacaga aaagaggaac gatgcaatgc tttgtctcaa
     5521 agcgcgggcg aaaatcaact ggacgctgga tattctgggc cggcgggccg acggctacca
     5581 tcagatggat atgctgatgc agtccgttcg gctggcggac accctctggc tggaggaaag
     5641 cgaccggctc acgctggagg acgcggggca gggctttcac accgaagcgg gggacggaga
     5701 ggcctgcgcc gcgcccgttc cttttgacga aaacaatctg gcggtcaggg ccgcccgggc
     5761 gctgcaaaag gccgcgggga tcgagaaagg cgcgcggata aggctgcgca aggcaatccc
     5821 ctccggcgcg ggcatgggcg gcggcagcgc ggacgcagct gcggcgctga tcggcctgaa
     5881 tcgcctgtgg aacttgcatt tttcgacagc ggagctggag cgcatcggcc tgaccgtggg
     5941 ggcggacgtg ccctttctgg tgcggggcgg tctctgccgc gccgagggcg tcggcgaaca
     6001 gctgacctcc ctgacccctg cgccaaagtg ctggctggtg ctctggcagc cctgcgatgg
     6061 actgagcacc ggcgaaattt tcacggcctt tgacacgacc tcggcggaaa cgctggcccg
     6121 ccctcagacc ctccgggcgg aggaagcctt gctggcggga gatttgcccg cattggcggc
     6181 ttccatgaac aatgtgctgg aaggggtcag cgcggcgaag cggccgcagc tggaacgcgc
     6241 ccttcaccgg ctggaagaac tgggcgcact gcgggccatg atgacgggca gcggctcggt
     6301 agtctacggc ctgttcgggt cggagggggc cgcaaacgcg gcgctttccg ggctggataa
     6361 ggaaaaaggc ttccgcgcag tgacctgcac ggaagcccaa ggggtggagt ttattcctcc
     6421 tctttcgtaa acccgaaccc ggcgatctcc gtcaccggca gggaaaacac gatggagccg
     6481 gcgggggagt cggggcccgc ttctttcaga atggaacgca tgatggccgt tttctgctcc
     6541 gtcttggtca agatcaggac gatttccttt tccgaggcca gagaaacggc gtagaatttc
     6601 tgggcgctat gagagccggt gcccttgccg tgcacaacgg tgccgccggt ggccccggcc
     6661 gcccgggcgg cgttcatcac cagatcggtg cagccctcgt tggcgatggc cagaatcagc
     6721 tcgtagtcaa aattcagctc cggggtatat ttgggcgatt gttcgccgct gagataagcc
     6781 aaggtctttc ctcctcccac acttttcacg ggtaccgcaa tggtgatgcc ctgccccggc
     6841 atccacagaa acagcttgcg cttcagctcc cggatcagct cccgggtttt ttccgggctg
     6901 gcaacgttca gaacgatgcg cttgaagttg ttctcgatgc ccagcagatc catcatgctg
     6961 cgggtggcgg tgccgcggcc gtgggtcgcg atgctcatgg gcatccccag ctgctggcag
     7021 agctccgtca tagcgtccag cgcgtccgga tggatgatcg aaatgatgta attcatcggt
     7081 tcacccccaa agttcgacga tatcgtcgtc gccgtattca ggctcgggct gcgcggcagg
     7141 cttgcggcgg ccgtagagca cgcccatcat ctggatggaa atcagcggca tcatggcaac
     7201 cagcgccacc acgccgaaag cgtcctgcag cacattgccg cccagcgccg cgctggctcc
     7261 catcatgaac tgcagcatga aggtggcggt catgggcccg gaggccacgc cgccggagtc
     7321 gaaggcgatg gccgtgtaga tgtccggcac aaagaaggtc aggcccagcg ccagcgcata
     7381 gccgggcacc aggaaccaca ggatggaaat gcccgtcagc acccggagca tgctcagccc
     7441 catggcgatg gcaatggcga tggaaaggct cagcttgatg gccttgccgg ggatcatgcc
     7501 ctcgctgacc tcttcgatct gcttttccag cacgtagacc gccggttcgg cagagatgat
     7561 gaaccagccc agcagcatgg ccagcgggat cagccagtaa taggtccagc cgctggtcag
     7621 ctccgcgccc agcaccgtgc ccagggagga aaagcccacg ttgaccccgg tcaggaacag
     7681 caccagcccc acatacgtgt acagaatacc cacgcagatc ttgaggaagg agcgccggtt
     7741 cagccggaag ctgcacacct gaaagatcag gaagatcag
//
LOCUS       DAWAEA010000021         7704 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_1550_length_7704_cov_2.546738, whole genome shotgun sequence.
ACCESSION   DAWAEA010000021 DAWAEA010000000
VERSION     DAWAEA010000021.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 7704)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7704)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7704
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1550_length_7704_cov_2.546738"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7305..7314
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1498 a         2423 c         2336 g         1437 t
ORIGIN      
        1 tgcggccagc atggccggcg tgctcatggt ggcgcccgtg ctggccaacg ccgccgttcg
       61 gcagggacaa aacctgctgc tttcctttcc ggaaaaggaa aacaccgccc ccgtgccggt
      121 ggagctgggg ctgcaggtcg cctttgaaga cgaatacctg atgatggtgg ataagcctgc
      181 acccctgccc tcggcttcct cgccccggca ggccctgccc acgctggaaa acgccgtcta
      241 cgcctatctg ggctgtccgg aggatttcgt ctaccggccc gtgaaccggc tggacaaagg
      301 caccagcggc ctgatgctgg tggccaagac cgcctacgcg caggatcaga tgcagcggct
      361 tttacacact gaccattttc agcgggaata tctggccgtc gccgaaggaa ggctgcccga
      421 aaaacaaggg ctgatcgacc tgcccatccg caaggccgac ggagccacag tgaagcggga
      481 agtcggcccg ggcggcaagg aagcccgcac gtggtactgg gtactgcggg aggaaaacga
      541 ccgcagtctg gtccggcttc ggctggatac gggccgtacc catcagatcc gcgtccactt
      601 aagcgcgctg ggctgcccca tcgtcggcga ttttctctat gggacggaat cggaggaact
      661 gccgggccgt ttcgccctgc acagctttcg ctgccgtttc ctgcacccgg tgaccggcca
      721 atgggtggaa cgggaaagcg acccgaagga gatttttgag cctctgctgg tgcgcagctc
      781 cacggcggaa gtgaagtcat aaactctcta taaaaggata tgcaaaagcg gtgggaaaat
      841 ttcccgccgc ttttatcgat tcattttttc agccgcttac ttattctcat acaccgaggg
      901 ggacaggatg cccacctccg gcaggttgcg gtacacctcg tcgtagtcca gaccgtagcc
      961 caccacgaaa gcgtccggga tcacaaagca ggagtagtcc accgtcagtt cggaaacgcg
     1021 gcgggcgggc ttgtccagca gggtggcgat cttcatgctg ttggcgcccc ggctgtacaa
     1081 aaggcgcttg agatacttca gcgtcatgcc ggtatccacg atgtcctcca ccaaaatcac
     1141 gtccttgccc ataatgctgc ggtcaaggtc cttaatgagc cgaacttcgc cggaggagac
     1201 ggtgctggcg ccgtagctgg acaccaccat gaagtcgatg gtcaggggca ggtcgatctc
     1261 ccggatcagg tcggcgaaga acaggctggc tcccttcagg atgcacacca tcacgggcgc
     1321 ttttccggca taatcctcgg tgatgcgctg accgagtttt ttgactgctt cggcgatctg
     1381 ctctttggtg aagaagatgc gttccatgtc ctgatacatt ttccgttcgt tttccattgc
     1441 cggtcactcc ttatggtctg atgataatcc agtgcagggg acagggcggc agcagagccg
     1501 ccataaacct tgggcggatg cgcggcgcag gctttccgag gaaaccatgc cggggatcca
     1561 caggatgtga tgatcgtccg ccagcagcca cagcgccggc cgaaggggcg cgtccagtct
     1621 gcggtcggtc aggaagcggc gcaggggctt ttcccccggc gctccgaagg ggcggagccg
     1681 gtcgccgggc agcggggttc tgaagcggca gcgctccacc tcgtcccggg tcagcagcac
     1741 ggcgtttgcg tcccggggca gcgggaagga gccgcttttc ggcggggtca tggtgatctc
     1801 ccagaaaccg ccgggcagcg gaaacgtcac cgggccgtcg gctccgttcg ctagcgtctt
     1861 taattccagc ggctccgaaa ccgtcagggg aaccagcggc tccccaccct gacggagcag
     1921 atgcagcagc ttttgccccc gcagcgcccg caaatcgccg ggcaaattca ttcccgcgcc
     1981 ggattccgct tgcaaaaggg cattgagttc cccgctggtt tcacgggaaa gctcccgttc
     2041 ctccgggtgc agcccggcca gcgcagcgcc ttcccggtag aaccgccgca gcgcccgcag
     2101 acgcagcgcg tcgggtccct cccggagagg gtcggtcaac agagcaaaca cgccatttcc
     2161 ctgccaatgg cagcggcggt acaggccgtc cgccagtgct tccagacaat cctcgtcctg
     2221 ccgcagcgtc tccgccgcag ccgccagatg ggaggccgcg ccgggaaaga gttcctccag
     2281 tcggggcatc agctccagcc gaagcgcatt gcggggcgta cagggcagat cgttgctttc
     2341 atcgtgccgg aagggaatgt tttcctcccg gagcacggct tccagttccg ctttggacaa
     2401 tgccagcagg gggcgcagca tcactccccg gccgaaggcc gtccgcggcg cgatcccgct
     2461 caggcccttt ccgcccgcgc cccgcagcag gtgcatcagc acggtctcgg cctgatcgtc
     2521 ccggtgatga gccgtcagca gcgcgtcggc ggaaagctcg tccatgcact gggcgaacag
     2581 cgcccgacgc ttttcccggg ccagcgtttc catgcccggg gtgttcatat cccccgtaag
     2641 cccggcctcc cggacagtga agggtacatt ccaacggacg cagagttctt ctgtcaggcg
     2701 ttgatccaac cgggtgcttt cgccccgaag gccgtgctgc acatggcacg ccgccagccg
     2761 aaagccgcct tcgtcccgaa gaagggtcag cccgtacaaa agtgccatgc tgtcgccgcc
     2821 gccgctgacc gccgccagca gcagggcgtt tttctgccac agacccgctg cctccagcga
     2881 gcgcctcagc gccgcaagca ggcgttcttt catgcctgac cttcccgaag catggcggtc
     2941 agttcttccg ccagcccctc cgtgtgctcc acggaagcca gccattgccg ggcgtcctgc
     3001 tcccggccca gcagcaggga caggaacagc ttcgtttttc ccgtgtgcag gcagacggct
     3061 cccgccgcct cctgcccacc aaacaggcac agggacggtt caccggggcg ggcggtcttc
     3121 agggcttctt ccagattgtc cgtccgacgg ggctgcaccg cttctgccgc aggaggcagc
     3181 gtgtacaacc gggtgatgag ccgggcggcg tactggcgga agtgccggct ggccgcttcg
     3241 caaaggcgct tggagtcggt gtcgatgcgc aggaacagcg tcgggtgttc gccctcggtg
     3301 acagagcagc tgagccggta tcggccccga tccgccagct cataggccgt gcggatctgg
     3361 cgtttttcac tcaactgggc ggcgtactgg ggcgcggcca gcagaatgcg gtcgcggtcg
     3421 ctgcggggca tccgccgctg gaacagttcc agtgcctccc gtcccccgcc cgttaaaaac
     3481 aaaacgccct gtactgcata ctggccttcc atcagggaac cgtccctcag gagttcgtcc
     3541 agatacagac acatgggcat gtattccatc aattccagct ttgccacaaa gggccagagc
     3601 tcctcgcggg tacaaaggcc cagagcgtcg atgcaaagaa gcagccgcag tcggttttcc
     3661 gcatcggaga tatggcgttt tgcgaatccc tccggcattt gctttccccc tctctggcct
     3721 tttccgcaca agtccggcga ataaagaaga aggatgcgcc gcaggcagct gcggcgcacc
     3781 caccaaatgc cctttcggtc accatttcaa cgagtgcagc ttggcctgcc cgaaggcagg
     3841 cgggcggcat taagccatga agggcttgat gtcgttgagc agatcatcgc aggaatcagt
     3901 gtagatctcc atggtgatgg gtttgctcag atccagactg aagatgccca gaatggactt
     3961 tgcatccacc acatgacggc ctgcacgcag atcaacgtca taaggataac gattcacgat
     4021 gttgacgaaa gacttcacgt tttcagccaa gctcagcttg atggtcacgg atttcatctt
     4081 gcattgtccc cctttctaat ttggaacgat gacattatac accagcgccg aaaaaaatgc
     4141 aagggtacgg gccggtcatt ccgtaagttt cagccatttt ccctcaaaaa gtacgttttc
     4201 cgcataagga aacttcgttt tcagggagcg ccgccagcat ttgctccgcc gcccccagca
     4261 gttcctcccg gcggttggga acgcgaccgc tgaccgcccg tttccacaga gcgtttagca
     4321 cgccgcccat gcggctcagc ggaactcccg ccgtgcggaa caggggcagc aggtcatccc
     4381 cggaaacggc cagtgccttc agcgacaggg gcgcgccgct ttcccgaagg gagcgaatcc
     4441 gtccggccag tccatcagcc tgttcccgct gtcccagggc cgagcgcatc tgacacacgc
     4501 cctccagcac ctccagccca gcgcgggcca gctgaacact cgttggttcg tccagcagaa
     4561 acgcgtccat caatcgaagg gccgcttcga cttcccggcg ggaaaagcgc aggtcttcca
     4621 gccttgcggc ggtctcggcc cggtcggccc ctgccagcaa tagcgccagc cgctgactca
     4681 ccggcgggag ggtttccatc cgggcctgat ttttcagggc ggacagattt tcctcccggc
     4741 agggcaggga acccagcagt tccggccatg ccccaatatg aagaatggta cgcagaccgc
     4801 tctccgtacc cgggcggcgg cgtttcagct gcggatagcg ccagtccgcc agcagggtct
     4861 tctgcaattc ctcccgcagg cgttcccggg cgatatcccg cagcagcggg gcatatcggg
     4921 tcaggctttc cagcagcgct tccgtcggct ccaagtccag ctccgcctga aagcgagcgg
     4981 cccgcaggat gcgcagaccg tcgtccttga gcacctgatc cgggtcggcg gtgacggtgt
     5041 gcagcacgcc ccggcggagg tgttccagcc cgccggtagg gtcgatcacc ggccccagcc
     5101 cgtccgggga aacctgccgg tacagggcgt tgacgctgaa atcccggcgc agggcgtcca
     5161 cggcgatgtc cgtggtgaag cgcacctgat ccggcttatg cccgcagcgg tagctgtcct
     5221 cccgaaaggt ggtgtattcg gccatatgcc gcgtaccatc cgggtcggtc aggtgcagtt
     5281 caacagtgcc gaaatgagcg gcccgcagat gggcccgcac ctccgtgccc tcgcacatcg
     5341 ccagcacggt ttcgggccgg gcggggccgc acacgtccac gtcgctgatg ggcagaccca
     5401 tcaggggatt gcgcaccgcg ccgcccacga tgtacagggg ggtatcagcc ccctgaaaca
     5461 ccgcggcggc gcgatggatc catgttgctt cgctcagcat ttcttttcgc tcaaggcaag
     5521 gaattcgttg atctcgccgc gcaccacgtc cagcgcgctg cgccaatagt ccacgctgtt
     5581 cacgtcgata cccacggagg cggccacatt acgcacggta tcgctgccgc aggaggccag
     5641 cagtttatcg tacttgggca cgaaagccgg gccttccttc tggaactgga cgaatacgcc
     5701 cagaccgaac agcaggccga aagcataggg gaagttatag aagtgcaggc cggtggagta
     5761 gtaatggctc ttgcacaccc acatggaggg atggcgcgcg tccttgctca ggccgtcgcc
     5821 gtagctctgc tcctgtgcgt ccagcatgaa gccgttcagt tcctgcgcgg aggggatatg
     5881 ggtcttacgg gcctcgatga cggcggattc gaacagatag cggctgtaga tatccaccac
     5941 ggtctgggtg gcctccatca ggctgttttc cagcagcaca agacgggtcg cgtcgtcggc
     6001 gctgcggcgg acagtctggc tcagcagggt ctcgttgaag atggaagccg tctcggccag
     6061 cggcatgggc gtctcggtca tcagcatggg cacagaggtc aggcagcggt tatgccacgc
     6121 gtggcccagc tcatgagcca gcgtgctcac gtcggagaag ctgcccacaa agttggtcag
     6181 cacccggctc tggtcgtgct gatggaagcc ctcgcagaac gcgccgcctt ctttgccctc
     6241 ggcggggaag aagtcgatcc agttgttgcg gaaggcttcg tccatcaggg cggcgatgcc
     6301 gggatgcacc ttggagaagg tgtcgatcag caggtcgtgg gcttcgtcca ccgtatagcg
     6361 gagctgcacg tcgcccaccg gggcgaacag atcatagaag ggcaggccat cgggatggcc
     6421 caacatttcc gccttgcggc gcaggtagcg tcggaaatcc ggcagtgcct cccggatggc
     6481 ggtcagcatg gcttccaacg tttcccgctc catgcgggtt tcggccaact gctgggtcag
     6541 cagatcggaa tagcccctcg cttcgcacag cgtcatggcc tgacccttga tggaggacag
     6601 gcagaaggcc atgggcgttt ccaccttggc ataggacttg agttcggcct cataggcctc
     6661 cttccgcacc ttggggtcag gatcgtaggc catgccgcgc acagcgggca gaggcaggct
     6721 ttcgccgttc agctccacgg tatggctgcc catcaggcgg tcacgcagct tggagaaagc
     6781 gtctccgccg tccagcgaca ggcgcagcgc ccacttttcc agatcggcgt ccagcatatg
     6841 ggcggactgt tccttatatt cccgcaggtg gaacgcaaga ctcttcaaat aatcgctctg
     6901 agcaatagcg ccttccaaat cgtccaccgc gccgatgaag cgaacccagg cgctgccggt
     6961 gagacgcacg tccaccatca gattactcag tcgatccagc agaagctgac cctgctggtt
     7021 attgcagtcg gtggccagcg aaagctgcac aaagccgaaa gtctggtaca gcagatcgtt
     7081 cagttccgtt tctgcctgca tcacggcggt cagtttttcc acgtcggagc cttcgctttc
     7141 ggggagcttg cgtccctcca gcgccagtgc gtggagcttt tcaatatcct tctcaagctg
     7201 cgggtcgtca aagcccttat aaaggaaggt aagatcccat tttccatttg ccatggtgtt
     7261 tccctctttt cgtatggatg atgtatattg aaaaagaacc attcnnnnnn nnnncccaga
     7321 ttatcctagc atggaaaagg tttcaatgca agaggggcga ggaagggatg aaaaaaggct
     7381 gacaggctcg ccagccctat gttatcatga aaacgtgttc ccgggaaacc aagagcacag
     7441 ggagagcgga taggctgaca ccagaaccaa acgccattca aggggcggaa gggaggtgag
     7501 agcctatgca gtccaaagca acggaagtga tcacgatccg caccaccgca acaacgacgg
     7561 taaagatcga gagaaccacg caggaaaaag aaagcaccac gatgcccgta atcatcgtag
     7621 tgctggacga taaaggctaa gcactaaaag cctttccgcg ctcctatctt accacacttc
     7681 ggaggtgaaa gtcaacggct gggg
//
LOCUS       DAWAEA010000022         7485 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_1602_length_7485_cov_2.591386, whole genome shotgun sequence.
ACCESSION   DAWAEA010000022 DAWAEA010000000
VERSION     DAWAEA010000022.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 7485)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7485)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7485
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1602_length_7485_cov_2.591386"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4347..4356
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1408 a         2364 c         2337 g         1366 t
ORIGIN      
        1 gatctgaacg aggtcttcga ccgggaaagc cacgtggacg agctgtgcga gacgctggcc
       61 gctcaccatg tggaccgggt gaaaaacaaa aaatgcacgc cgaaaaacgg aatgctctat
      121 ctggatatgc tcaacaatct ggaacgcatc gccgaccacg ccaacaatct ggcggagtcc
      181 gtcgatcaca agggcgaccc cgaccggctg ctgtggtaaa aagcaacacc ggcgcgaaag
      241 atattctctc ccgcgccggt gtttttattt ttccaatgaa atttcccgcc cgtccacggc
      301 ggcggctgtc agcgcgtcgt ggatgtaggt caatttcaac gagaatacgt cctgccgctc
      361 ctccagcagc cggaagaaaa gcttgtcccc ctcccacagg ggaaggctgc cgatcttctc
      421 tttttcgatc cattggaggt cgccctccgt gcaggggatg ggctggcctt caaagccgtc
      481 ggcggtatac aaaaaggtat attcgtccag atcgtcaatg acgaaggtaa tcacgccccg
      541 cagccgaaag cgggtcagcg tatagcccgt ctcctcccga acttcccgca ggatgcattc
      601 ctccggggat tcgccctgtt ccagatggcc gcccacgccg atgtacttgt cgtggttcat
      661 gtcgtttttc tttttggtgc ggtgcagcat caggtagcgc ccgtcctttt ccagatagca
      721 cagcgtggtc aggatcttgt tcatggcttc ggtcttcctt tcgcttcttt cgttccacgg
      781 aggtattata gcacagttgc cgctctggtt gcatccggga gaagaaacca gtataatgga
      841 acggccaata gggctgtttt cctcaacaac aaaagaaagg ctgaacgatc tatggacatc
      901 tttgcatatc tggaaggcat ggcggctcag cacgggccca gcggccacga ggcttccgtg
      961 gcccagtggc tgagtgagcg cttccgcccc ctgtgcgaca gcgtgaccat cgacgcgctg
     1021 tacaacgtta tcgccgtcaa aaaggccact ctgcccacgc ccgacggctc ccccgctccc
     1081 cgggtgatgc tctgcgccca tcaggacgag attgccctga tggtgtccga cattctgccg
     1141 gacggcaccc tgcgcatggg ctccgtcggc ggcgtggatc cccgcatcct gcccgcctcc
     1201 acggtgacgg ttcacgccac cggcgagggg gaaacgcgaa agctgaacgg cgtggtgggc
     1261 gcgctgccgc cccacctgat gagcgccgag gatcgaacga aggcctatac ccgggaggaa
     1321 ctgttcgtcg acctgtgcat ggccccggaa aaagtgaaaa agctggtgaa catcggcgac
     1381 ctcattaccc tgcagggccc cgccgtcaag ctgctgaacc accgcgccgc cgccaagacc
     1441 gtggacgacc gctcctgcgt gggatgcctt ctgctggccg cggaacggct ccagaaaatg
     1501 cagcatatgt gtgatattta ctttgtggct tccagtcagg aggaagtcgg cggccggggc
     1561 gcggcggtag cttcctatac cgtagacccc gatctggccg tggtgctgga cgtgaccttc
     1621 gctcccacgc cccagtgtcc gccggatacc acctgcccgc tggacgcgcc tgccaccgct
     1681 tacggcccct atgtgcagca ccggctgctg gaacggctga agcagacggc caaggaaaac
     1741 ggcgtcaccc tcaactgtga gcatacggag cgcagtaccg gcaccgacgc ggacgacgtg
     1801 cagatcgccc gggcgggcgt tccctgcgta ctgctcagtc tgccggtgaa gtacatgcac
     1861 accagcgtgg agctgttgga catgaacacc ctgtccgaat gcggccgtct gctggcgctg
     1921 tttgtggcgg gcattgacga gaaatgggag gatggcttat ggaactgagc aggctttgtg
     1981 aaatgcaggg catcagcggc cgggaagaac tggtgcgcat ggccattttc cgggaatgtg
     2041 cggaagtact gggcgaggaa aatgtgaaga tcgaccgaac gggcaacgtg atcgcccaca
     2101 aggcctgcgg gaaagcggac gcgccgcggg tgatggtggc cgcccacatg gacgaggtgg
     2161 gcctgatggt catcaacgcc gcggacgacg ggcttctgca ggtgcggaat atcggcggag
     2221 tcgacccgcg ggtgctggtg agcaagcggg tcaaggtggg ttacgacgtg cccgtccggg
     2281 acggaaagcc cgcgcaggag gctctgaacg gcgtgatcgg cgcgatggcg atccacctgc
     2341 agagcgccgc cgaccgtaaa aacgtgctgc ccattgaaaa cctttatgtg gatatcggcg
     2401 cgaaggacaa agacgaagcg ctggccaagg ccccggaggg aacgcccatc acctttgcca
     2461 ccccctccac ccccatcggc gacgggtgcc tgctgtgcaa ggcgggcgac gaccgggtgg
     2521 gctgctacaa cctgctgcgg ctccttcatg ccgacgtgac ctgcgatacg tggttcgcct
     2581 tcacctgtca ggaagaagta ggcagccgtg gcgcttacgg cgcggccttc cgcattcagc
     2641 ccgacgtagc cctgattctg gagggcacca ccgccaacga cgcgggcgag caggacgaga
     2701 cccggaaggt ctgccggctg ggcaagggcg tggccgtcag ctttatggac aaagcctcca
     2761 tagcgcagcc ggaactgttt gcggagttgc tgcgtctggc cgagggcagt ggcattgctc
     2821 atcaggtgaa aatggccgtg gcgggcggca acgacagcgg ccccattcag cggacgggct
     2881 gcggcgccaa ggtctgcgtt ctcagcgttc cctgccgcta catccactct ccgtccaccg
     2941 tgctttgtct ggaggacgtg gaagcccaat acctgctggc gaaagcctat ctgacccgct
     3001 gacttatact gaaacaaatc gatcggggga atcaagccat gtatgaactt ctgaaaactc
     3061 tgacctccgc ctacggcgct tccggccggg aaacgcccgt cgccgacgtc atcgaagaac
     3121 tggttcgcag ccatgtggac agcgtccgcc gggacaacat gggcaatctg atctgcgtca
     3181 aaaacggcac cgaccccaac ggaaaacgca tcatgctttc cgcccacatg gatcacatcg
     3241 gctttatcgt ggccgctgtg gaaaaagaag gcttcctgcg ggtagctccc gtaggcggca
     3301 tcaaccctgc cgtcagccag acccggcacg tcagctttga aaacggcgta cagggcgtgc
     3361 tggtgcgtca gcccctgaag gagggcgaga cggtcgccat gaagcatttc tacatcgaca
     3421 tcggcgcgaa ggacgcagag gaagccctgt ccatggtgca ggtgggcgac gtggccgttt
     3481 acgccaacga ctgtttccgg ctggggcagc atcgggtctc cgcccccgcc atggacgacc
     3541 gggtggcctg cgcgctgctg gtcagcctgc tcagcgatct gccttctgtg aaggacacgg
     3601 tcatcgccgt gttctccacg caggaggaac tgggactccg cggcgcaaaa acagccgcct
     3661 acggcgtgga gccggacgtg ggcattgcgc tggacgtgac cgccaacggc gacacgcccg
     3721 aggacaagct gcccgccgtg aagctgggcg agggcatctg cgtcaaaatc atggatcggg
     3781 gcagcatttc caacccggag ctggttcggg agctgctggt tgccggtgaa aaagcgggcg
     3841 tgcccactca gcgggaagtg ctccccttcg gcggcactga cgcgggagcc atgcagctga
     3901 cccgcggcgg cgttcgcgtc tgcaccctgt ccatcccctg ccgcaacgtc cacagcgcct
     3961 gcgaggtcat cgacctgcgg gacgtggatg gcggcaaaaa gctgctgctg gcctatctgg
     4021 agcgttaagc gccgcccggc gtctccgctc tcaacgcttc ttgattgcac aggccctccc
     4081 ctgcgggagg gcctgtcgct tttcccccgg ttgacgcggc ggcaaaaggc cgtataatga
     4141 aaccatcccg accaaagaaa atggagtaca cttatgaacg cttcaaatcc ttccatggaa
     4201 aaagccccgc gccatatggg gatcgagctg ctgcgaatcg tggccaccgg catgatcgtg
     4261 ctgcaccatt tgctagccca cggcgggctg ctggctgtct ttcccagccc cgggcccacg
     4321 gatgcggcca tgcgcctgct gaacgcnnnn nnnnnncctt cgcttactgc gcggtcaacc
     4381 tgtacgggct gatctccggc tacggcggca gcggacggtt tcgtccggcg cggctggtag
     4441 ggctttgggt gcaggcatgg tttaccggcg tggtgctgac ggcgcttttg tcgccgctga
     4501 ccccggtcac ggcggcggac tggcgcacgg ctcttttgcc gattggttcg caggaatact
     4561 ggtacctgac ggcctatttc ggcctgtatg tgctgtctcc cgccctgcac gctgccgtgg
     4621 agcggctttc ccgccgtcag ctgacgttga cgctgctggg ctgcgccgtt ctgttcaccc
     4681 tgacgccctc cgcggcggaa tcgctgagcc tgagtggcgg gtatcacgtg ggctggctgt
     4741 gcgtcctgta cctgctgggc gcctggctgc ggcgatacgg ttcgcccgcc cgttccaact
     4801 ggaagccgct gctgtgcttc ttcctctgcg tgggaatcgc gggcggatgg cagatcgccg
     4861 cgctgctgct caaggcccgg ggcattacgc cgcttatgga cggcgatcaa ctgctgcact
     4921 acaacagccc gttgatgctt ctggcttcgc tggcgctgtt tcggtggttc ctgcgtctgc
     4981 atccgtcggg ccgtctggaa cgcttcgtcc gctgggccgc gccgctgacg ctgggcgtgt
     5041 atctgatcca cgatcagccc gttttccgcc gggtgttcat cgtcctgcgg ctgaccggat
     5101 tcagcagtct gcccaccctg ctgatccccc cggcgctgct tagcgtatgg ctggtagtct
     5161 tcgccggttc ggcggcgctg gaatggctgc gcgttcggct tttcgccctc tgccgggtcg
     5221 accggctctg ccagcgggcc ggagaccggc tcaccgcgct ggcctgccgg cttttgccgg
     5281 aagaaaaagc ccgcccttga cccatcccgc tccatggact acaataagga aagaacctgc
     5341 ctgtccgtca ttcctacgga aatggcggac ggcagaaacc caacagggtt ccattcggaa
     5401 ggaggacatt tctcatgagt atctcccgga aatattttga caaaaccgcc cgcggcgagg
     5461 aagtgaccca atatacgctt accaatcaca agggagccag cgtcagcgtc attgacttcg
     5521 gcggcattgt gaccagcatc gtggtgcccg accgggacag aaagctggcc gacgtgaatc
     5581 tgggcttcga cgtggtggat acctacgacg gcaagggcgg ctgcatgggc gcactcatcg
     5641 gtcgggtggg caaccgcatc ggcggcgcgg cgttcgatct ggaaggccgc cactacgtgc
     5701 tgggcaagaa cgacggagaa aacaacctgc acggcggccc ggagggcttc agccagcgga
     5761 tgtggaaggc cgagcccatc gagggtcggg acggcgacac cctgcggctg accctgacca
     5821 gccccgacgg cgatcagggc ttccccggca agctggacgt gaccgtggac tacacctgga
     5881 acgacgcctg cgagctgggc atccactaca tggccgtcac cgacaagccc accctgtgca
     5941 acatgaccaa ccacgcctat ttcaatctgg acggtcacga cgcgggcacg gtggagaacc
     6001 tgaccctgca gatcaacgcc gacagcgtta ccgaagccga ccccgcgctg atccccaccg
     6061 gacggctgct gccccagaag gggctggcct acgacttcac cgaagaagtc cggctgggcg
     6121 acgtgctggc caagaccccc gtggatcccg tcatgcggga cgcgggcgga gtggacttca
     6181 actactgcgc gggccgggac cgggagacca agctgatcgc caccctgcgc tcccccaaga
     6241 ccggccggcg catggacgtg atcaccgacc agcccggtgt gcagtgctac accggccagg
     6301 ggcttgacca caccggcaag ggcggcgttc actacggccc ctacgcaggc gtgtgcctgg
     6361 agacccagca ctatcccgat gcgatccatc agccccattt cgaaagctac gtgctgcgtc
     6421 ctcaggacaa gtacgacacc ttcaccatct tccgcttcac tgcggaataa gcagtcgagg
     6481 gctttccgcc cggaaggagg cgcttgatgt ccactcagtt tcgcatgacc gacggcccgc
     6541 ttttggacgc tcagggccaa ctgatcgaaa aaggctacca gacccgtctg agccgcgtct
     6601 acgaccggag cgccatccgg gcgggagcgc tgcgcatcaa ggaatgggac tactattgcg
     6661 tgacctccgg cagcaaagtg ctggcgctga ccattgcgga caacagctac atggggctgg
     6721 acagcatttc cctgctggac gtagacgcag gctggcagca tacccgcagc atcatgcggg
     6781 ccttccccat gggcaagacc ggcctgccct ccaccagcgc caggggggac gtttgcgtca
     6841 gcggcaggga ttacttcctc cgctttcaaa acgacggccg cgtccgggtg ctggacgcgc
     6901 agatgggcaa ttttcagccc ggcaagtccc tgaaggccca tctggtgctg acggacgagc
     6961 cggaggactc catggtgatc gccacgccct ttgcgggcaa gccccggcac ttctactaca
     7021 atcagaagat caactgcctc tccgccgagg gcgaggtcgt ctttgacgga cgggtctatc
     7081 ccttccgcaa ggaagattcc atggcggtgc tggactgggg ccggggcgta tggacgtaca
     7141 aaaacacctg gtactggagc agtctgagcg cccgtctgga cggcgtgccc ttcggcttca
     7201 atctgggcta cggcttcggc gacgtcagcg ccgccagcga gaacatgctc ttctacggcg
     7261 gcagggcctt caagctggac aaggtggagt ttttcattcc gcagaccccg gacggcaggg
     7321 acgattttct gaagccatgg acggtcgcgg acaacgaagg ccgtctgaac ctgaccttca
     7381 cccctgccct ggaccgggcc agcctgacca gcgccctcgt catcgaaagc gaccagcatc
     7441 aggtcttcgg ctactttgac ggcgacgttc gcttcggcga tacgc
//
LOCUS       DAWAEA010000023         7381 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_1622_length_7381_cov_2.132269, whole genome shotgun sequence.
ACCESSION   DAWAEA010000023 DAWAEA010000000
VERSION     DAWAEA010000023.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 7381)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7381)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7381
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1622_length_7381_cov_2.132269"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1214..1223
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2567..2576
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1402 a         2258 c         2224 g         1477 t
ORIGIN      
        1 gccttggcga agccgccgtc caccaccttg cccagctcgt ccatattgtg gacttcgtgg
       61 atgtgggcct gctggaaatt cagcgccagc tgatacatgg tcttctggat atcgtccagc
      121 agtgcgggga tcacgctggt cagttcgtcc agcttggccg tctgcttttc gtaggtgtcc
      181 cggcggaaca gcacgcactg gccgttttcc agatcgcggg ggccgatctc cagacggacg
      241 ggcacgccct tcagctccca gtcgttgaac ttgaagccgg gggacagggt gtcccggtcg
      301 tccagcttca cgcggacgcc ttcggcctcc agtgccttct gcacctgagc gcacttttcc
      361 agcacgccgc ccttgtgggc cgcgatgggc acgatgacca cctgaatggg agcgatacga
      421 gggggcagct tcaggccgcg ctcgtcgccg tgggtcatga tgattgcgcc gatcagacgg
      481 gtggacacgc cccagctggt ctcgtagacg tattccagct tgccttcctt attggagaac
      541 tggatgttgt aggcttcggc gaagttggtg ccgaagttgt gactggtgcc ggcctgcagg
      601 gccttgccgt cctgcatcat ggcctccatg gagtaggtgg cccgggcacc ggcgaatttt
      661 tccttgtcgc tcttctggcc caccagcacg ggcatggcca gctcgtcctc ggccacctcc
      721 cggtagacct ccagcatgtt catggtctcc tcctgcgcct cctgcgcggt ggcgtggatg
      781 gtgtggccct cctgccacag gaactcgctg gtgcgcagga aggggcgggt gctcttttcc
      841 cagcgcatga cgctgcacca ctggttatac ttcatgggca gatcgcgcca ggagctgacc
      901 cacttggagt acatggcgca gaaaatggtc tcgctggtgg gacggatggc cagacgctcc
      961 tgcagctttt cgttgccgcc ctgagtgacc cacgccactt cgggagcgaa gccttccacg
     1021 tgctccgctt ccttcaaaag caggctttcg gggatcagca tgggcatata gacgttttca
     1081 tggccggtgg ccttgaagcg cttgtccatt tcctcccgga tgcgctccca gatggcgtag
     1141 ccgtagggcc gcatgatcat gcagccgcgc acgggggcgt agtccaccag ttggcttttc
     1201 aaaataacgt cggnnnnnnn nnngtgtacc actgagaaaa atccacgtcc cggggcgtaa
     1261 tgtgctccac aaaaccctgc ttcttttcgt ccgccattgt tttttcctcc tgttgatcgg
     1321 ctcgtttggg tatgaaaaaa ggcttcccgc tcccacgcac agccctgccg tgcgccggga
     1381 cgaagggagc cttcgcggta ccacccagct taaaacgcgc gcagcgcgcc gttttcacct
     1441 cgtgtgcgcc ctgtatcggg ggcgggccgt cggggattgg ccgacttcgc caaaggcggg
     1501 tccttcgccg ttctccgccg ccctttcagc cgaggagcgg ctctctttgc gaaaaaacga
     1561 tgcgaaatca ttcctttggg ccgggcgcag ggcccggaac agtgcctatc ttatcacagc
     1621 ggggcgggtt ttgcaagcgg aatcccccgc gttttcactc tttcacgacc tccacgcttt
     1681 ccacgccgca gcggcgaagc agcgtcacag cctccgggcc gatgcgctcg ccggacacgg
     1741 cgatggggat ggccggcggg cacgccaccg tgggcgagcc gcagacacgc cccagcgatt
     1801 tttccgcagg cacgcgctcc tgtgggcgca gcatggcttc ccggatggac atttcctgcc
     1861 ttgccttggg cagcggcagg ttttccggcg gcttaggcgg acggtcgttc cggcccagcg
     1921 cttccaccag ccgccccagc tcctccggac ggttctgggg cgtcagcatc agcaccagat
     1981 ggtcgccgtc ggcgtactcg cactccatcc cggctgaacg cagcagccgg gccagcccgg
     2041 ttcccgtctg tcccgccggg gcccgcaggg tcagccgcag ggggtcgctt tcctccgcct
     2101 gccagccgtt ttgccgcagg gtttgccggg tctcgtccaa aagggccgtc cattccgcca
     2161 atttccgacg gaacgccccg gccagatagg cgttgcaccg atccagtgag cacagcgtca
     2221 ggtaggacgg actggtggaa ccgaacagcg ccagtgcctg cctggattcg ccgcaaagct
     2281 cgtcagagac tcggcggctc aaatgcagat acgcgccccc ggtcagcacc ggcagggtct
     2341 tgtgggcgga atcgcagcac atgtccgccc ccagatccag cggatgcagg ggcttttcca
     2401 gaaaatgcag gtacgcgccg tgggcgttgt ccaccagcag cagcgtatta tgccggtgac
     2461 agacctccgc aaggccctgc acatccgcca tgccgcccag atagtcgggg ctggtgacgt
     2521 acacggcggc gagcggcgaa ggaagcgctg ccagcgcgtt ttccagnnnn nnnnnnttcc
     2581 agctgagcgg gcgaaatgcg gcaggaacac agggaatccg tcttctccgg ccacagccag
     2641 cgcacctcca gccccagcag cgccgctccg taaacgaaag ccttgtggac gttccgcgcc
     2701 gccaaaatca gcggcgtacc gccggtttgc cgcagcatcg ccagccgcag catggcgcgg
     2761 atgcactggc tggagccctc cgtggaataa aaggtacgcc cagcgccgaa cagggatgcg
     2821 gcgttccgtt cgctttcggc gatgatcccc ctcgcgtcgt acagctcgtc cgcaccggcg
     2881 atttccgtaa tgtcgaagct ctcgcagccc agaaagcctg cgcccttgtg gccgggcata
     2941 tggaagcgca ccatgccgga ctcctgatag cgccgcacaa aatcaacgat cggcgttttc
     3001 attctcctgc gcgtcctctt cggcgatttt catcataata gcgcactcga tgcgcttgcg
     3061 gaacagctcg cagcccggct cgtaaatgcc gcggatgctg cccgtggcgt gataggcgtt
     3121 ggcggcgcat ccgccggagc aatacagccg cgcccagcag tcgtcgcatt ccttccgggc
     3181 gtaggcgttg caggaacgga actcctcccg cagcgccgtg ttggtcacgc cctgccagat
     3241 gtcgcccagc ttgtactttt cctctcccac gaactggtgg cagggataca ggtctcccca
     3301 gggggtcacg gccatgtact ccgtgccgga gccgcagccg gaaatgcgct tgtacacgca
     3361 ggggccgccg atcaggtcca gcatataatg atagaaggtg atgggcctgc cctctttttt
     3421 ccggcgcagc atgtccttgg ccagaatttc atactgctcc ttcacaattt ccagatcctc
     3481 cggtgtcaaa gcagcgggat cgtcgggagc gcagaccacc ggctccatgc tcagctcggt
     3541 aaagcccaga tcggccatgt ggaacacgtc gttggtgaag tcagggttgc ggtgggtaaa
     3601 ggtgccgcgc atgtagtagt tctttccccc gcgggccttg accagcttct ggaatttggg
     3661 cacgatgcgg tcatagctgc ccacgcccgc gtagtctacc cgggtctggt cgttgatctc
     3721 cttacggccg tccagcgaca gcaccacgtt gctcatctct ttgttggcga agtcgatcac
     3781 atcgtcgtcg atcagcatcc cgttggtggt cagggtaaag cggaaattct tgccccgctc
     3841 cttttccacg ctgcgggcgt actgcaccag ccgtttgacc acggcccagt tcatcagcgg
     3901 ctcgccgccg aagaaggcca cctccagatt ggttcaggtt gcaggtatgg gaaacgtgca
     3961 ggcacagggc cttgaccacg tcgccgctgc gctccttgaa ggtgcccgcc agcggagcga
     4021 aggtgtccgg gctgaaaagc ttgccttctt tgaccagttc ctccacgtcg gcaatgcact
     4081 cccgcacgtc ggcctcggtc acgtcctccc ggcccgcgta tttgtcgcag atcagccgga
     4141 cgatctcctc cggagtgtgc ttgtcgtaaa gggcgatgat atcgtaggcc acttcgtcca
     4201 ccgcgtggat ggagccggag caggagtcca gcacgatgtt gtagccgtta agctgatact
     4261 gatgaaccac gcttggttcc tccgttcttt cgcagatgcg caaaaaggct gccgcagcaa
     4321 tcacggcgcg aaccgccccg cattttgccg caacagcctc ctgtggtttc caaaattact
     4381 tgttcttgtt ttcgcacttc tggttggcca cgccgcagct ggtcttgcaa gcagactggc
     4441 aggaggtctg gcattcgccg cagccgccct ttttggcgct ctcgcacagg ttccgggtgg
     4501 cgatggtctt gatcctcttc ataattcgca tctcctattc gtttcttccg acacgtaaag
     4561 cgccggagta ttggctttta caacaacagg gagtatagca cacgtctttg ggaaagtcaa
     4621 atgctatcca tggttttcaa tcctttgcgg aaaaaaatgg aaaaaatatg gggatcgccg
     4681 cgccggtcag aaccggcctt tttagaaaag cgcagtaacc gctcgccggt ttgcgcaggc
     4741 ctgatcgaca aaaagcgcgg aaattttttg cttgacattc cttcccgttc cccttatgat
     4801 gagaacagaa atacgaacat gtgttcgtat ttgaaaaagt tgttctgccg ggatcgggcg
     4861 ttgaaaatcc aaatcggatt ctctaaaatg cgcccatccg gcggcggatg caatgccccc
     4921 tgtgcgccgg gcgaggccat gcgctgtgac ggaaagggga gcggaaagga aagcgtatgc
     4981 cggaagaaaa ggaaacctgc atgaactcat acgacctgcc catttccctc caagataagc
     5041 tgaagcgggt ggacgagatc caccgcgcca ttacacgggc ggtggggaga atcgaatatc
     5101 tgaagctctc cgccctgcgg gccggcagcc gcccgacccc tgtgcgctac gggcgcggcg
     5161 tggaacgaag ccgcgtggag tacgcggtgc tggaaagtga cgccgagcgc tggggcatcg
     5221 gcgaactgca aaaggagctg gaggcgctgc tggaggacat tcgcccgctg gtggagaggc
     5281 tgcccgccgg agtgctgcgg gccgtggcca cccagcgcat tttggagggc atcccctgcg
     5341 gcatcatcag ccggaggatg catttcagcc gctgctatgt gtaccggctg ctggatcagg
     5401 cctgtgaccg gctctgccag atggagaaag cccggctggg gctgaagaaa aacgaaaaat
     5461 aaaggaaggc aatggaaaag gcggacaatc ggttgtccgt cctttttcat aaagtaaaaa
     5521 ttatgtaaaa aaccttcggg ggaggacttg catccctctt tatccggtgt tatcatgagg
     5581 acgttcggaa taatacattt accacgaaag cggggatata gaatgatttc ttttggtaca
     5641 ggcggttggc gcgccattat cggcgatgac tttaccaagg aaaacgtgcg caagctgatg
     5701 ttcgccctgg cccagcggat gaagcgggag gacgtggccg acaaaagctt ctgtatcggt
     5761 tatgaccggc gttttctgag ccgggaggct gcggaatggg ccgcggaggt catggcgggc
     5821 gaaggcattc acgtgctgat gatcaaccgg gaggccccca cgccgctgat catgttcacc
     5881 gtgcagtatt acgggctgga ctacggcatt gcggtaacgg ccagtcataa tccggcgctg
     5941 tacaacggca tcaagctctt caccaagggc ggccgggacg cggtggaaag cgtgaccaac
     6001 gaactgggcg cggcggccaa cgacgtcgat cccaaagaaa tccattccat cgaatacaag
     6061 cgtgccatcg cggcaggtct ggtgcaggag atcaatcccc agaaccagta tctggacgcg
     6121 atcatccgcg gcgtgaacat ggacgcgatc cgcagccgca acctgcagat cgtgctggat
     6181 cccatgtacg gcgtgagccg cacgtccctg cagaccattc tgttgacggc tcgctgcgac
     6241 gtgagcgtca tccatgagcg ccacgatacc ctgttcggcg gacggcttcc cgccccgaat
     6301 gtggacacgc tgggcgcgct gcaggcggcg gtgctgcaga accacgccga catcggtatt
     6361 gccaccgacg gcgacgcgga ccggctgggc atcatcgacg acgaggggca tttcgtccac
     6421 cccaatcagg tgctggtact tttgtactac tacctgctca agtacaagaa ctggcacggc
     6481 ccctgcgtcc gcaatatcgc caccacccac ctgctggacc gggtggccga ggctttcggc
     6541 gagacctgct acgaagtgcc ggtaggcttc aagcacattt ccgccgccat gcaggagcat
     6601 aacgccgtca tcggcggcga aagcagcggc ggcctgacgg tgcggggcca catcaacggc
     6661 aaggacggca tctacgccgc caccctgctg gtggaaatgc tcagcgtcac cggcaagtcc
     6721 atgagccagc tgatgaagga gatctacgcc gagttcggca ccgcctacat gagcgagttc
     6781 gactggccct tcgatcagga aatgaaggaa aagctgcacc acctgctgat ggtggaaaag
     6841 cagctgcccg ccttcccgat ccccgtggtg gccaccaact acatggacgg ctgcaaggta
     6901 ctgttcgaga acggctggat catcgcccgg ttcagcggca cggaaccccg gctgcgcatc
     6961 ttttgcgaaa tgcccacccg ggaggaagcg gagcgcctga ccggcgtgat ggcggatttc
     7021 ctcggtctgc ccatgcccgt caaaatgatt tgatctgtgt aacagtttcg ccggagcaaa
     7081 ggattgtgcc tttgccccgg cgaatttttt gattcggttg aaaaacctac gataatagcg
     7141 aaagcaggtg aaacccttga aatttcatca gaaaatgctg gccctttgcc tgacgctggc
     7201 cgtgctggcc tgttccgttg cagcctttgc cgaggaaagc acggcgaccg ctcccctgcc
     7261 ctgcggcgtt cgcttcggca tgagtctgga cgagctgagc gccttgctgg gcgaggacgc
     7321 actggtcgaa atctgggacg acgacgcagg cacaggctcc gtttccatgg aaaacgtcaa
     7381 a
//
LOCUS       DAWAEA010000024         7227 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_1663_length_7227_cov_2.138176, whole genome shotgun sequence.
ACCESSION   DAWAEA010000024 DAWAEA010000000
VERSION     DAWAEA010000024.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 7227)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7227)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7227
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1663_length_7227_cov_2.138176"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4056..4065
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6816..6825
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1346 a         2228 c         2338 g         1295 t
ORIGIN      
        1 ctctacgctt ccggcgcgcc ctacgactcg ctggaggagg ttatcggcct tcggggcgac
       61 ggagcctgct ccctgaagga aatgaacgtc agctacgcgc aggtggaaat gaaggacggc
      121 aagctcgatc tggctgccat ccgcacggcc atgaagccca acacccgcat ggtggcggtg
      181 cagcgcagcc ggggctattc cacccggccc agcctgatgc cggaggacgt ggagccgctg
      241 gctcggatgc tgcacgagga gtttcctaac gcctgcctga tggtggataa ctgctacgga
      301 gaattcgtct gcgaaaagga acccagcgaa tacggcgcgg atctttgcgt gggcagcctc
      361 atcaagaacc ccggcggcgg gctggctccc accggcggct atatcgtggg ccgggaagac
      421 ctgatccgcc gaatttcctt tcgcctgacg gcccctggca tcggccggga ggagggcagc
      481 tacgccgcca gctaccgtcc ctattatcag ggcctgttca tggcccctca tacggcggtg
      541 caggcggtca agacctctat tctggcggcg gcggtgtttg aagaattggg tatgcgctcg
      601 tctcccacgc cggacgctcg ccgcacggac atcattcagg cgctggaatt cggcaccccg
      661 gagcggatgg tggccttctg tcaggccatt caggccgcgt cgcccatcga cagcatggcg
      721 gagccggagc cttgggatat gcccggctat gccgatcagg tcatcatggc ggcgggcacc
      781 ttcgtgtccg gcgcatcgat tgagctaagc gccgacggcc ctatgcgcgc gccctacgcc
      841 gtgtacctgc agggcggcct gacctacggc cacggacgca tcgccctttg ccgtgcgctg
      901 gaagccatgc agaaaaaggg cactctgtaa aaaacatatc ccctcccgga agcgcatagt
      961 tttccctggg aggggatttt tgtgaaggaa cagacccgcg taaaggccgt gcggctgcgg
     1021 caggaggcgc cgcggacgga cgttttgaaa cctgtggccg cgtcacggcc cgcagaatgg
     1081 atcgacgctt cccaaaagaa ggatggattt tcccggctgc tgcgggcgga tcggctcatc
     1141 cgcaatctgg ctgctgctgc ggggctgctg ctggtggcgg tggcggtcaa aaacgccgag
     1201 ctgccccaaa gtcagtccgt gttttctgcg ttggaggaca gcctgaacac cgagtgggaa
     1261 gaaagcgtag gcaagctgag ctttgtggac agcctgctgc ccgctgaagt gcaggaggtc
     1321 tggcggcagg aggaaagcgt gacggtcttt gagccggtac agggcaatgt cgtccacgcg
     1381 tggtcccggc aggaacccta tctggaactg agctgcaata ctacagatgt gcgtgccgcc
     1441 gcggatgggg agatcatgtc tatcgcccat ggaatggacg aggaacgcat cgtccgcatc
     1501 cgccacgaag gcgggctgga aaccctgtac ggcaatctgg caagctgcgc ccacgaggtg
     1561 ggggaccggg tctatgccgg ggacgccttc gccacggtgc tggaaggcgc gccgctggcc
     1621 ttcgagctgc ggcggggcgg acgctccatc gaccccaccg ggctgattct gcccctgacg
     1681 gaatgagagg caagcggatg aagctgcggc tgaagattca cccggcaggg gccgtgctgg
     1741 cggcgctggg gcttatgctg gttccctcgg cgggaatgct ggcggcggct gcggccatgc
     1801 tgctgcacga gggcggccat gtgctggcca tggccctgtg ccgggtgaag gaatgccgca
     1861 ttgaactgac ccccttcggc ggcgtggcgg acgtgccggg ctttgagcgc ctgccgggca
     1921 ggcagcaatg cctgatcgcg ctggcgggcg tgccggtcag cgcggcgtcc gcatggctat
     1981 gtctgcgttt tgcaccgcag acggagtttt tccgcctgtt ttaccgcttc agcctgggca
     2041 tggcgctggt gaatgtactg ccggtctggc cgttggacgg ggcgcgggtg cttctgtgcg
     2101 cagcacggtg gtttggccgg gaggaaacgg cccggcgggc catgctctgg ctttcctatc
     2161 tgctggcggc gggaatgctg gcgctgggcc ttgccggggt ctttcagggc cgggtgaacc
     2221 tgtcgctgtt cctgctgccg ccgtatctgg cctacgcggc atggcagagc agcatgggcg
     2281 gaagtctgcg gggcgcggct gcggcactgg aaggagagcc ttggcccaaa ggaaaactgc
     2341 tgcgggcgct gccctttgtc tgccggggaa cgccggaaaa gttggaactg cttcgtctgc
     2401 tgcgcgccct tcccgagggg cgcacggcgc ttctttacaa gtttgacggg caaaccggca
     2461 ggctgacgga agtgcttgcc gcaccgaaga tcgccgaatc gctgacccgg caggacaaag
     2521 aatgaaaagc gccggcgctt gtccaaatcc agcaatttcc acgcggattg gatggacaaa
     2581 gcgggcgttt ctgtggtaca atctgaatga ttacgaaagt gttacgaacg gaggcacgat
     2641 tcatgcgaaa gcacacggca ctgcaaaaga tcgccgccct tctttcggct ctctgtctgc
     2701 tgatgggcgc ttttcccgca atggcggcgg acgtgggagg cgtggcggtt cagtccgccg
     2761 ctttgaccac ccagtctgcg cccctgaacg gcatggtgcg ggtctggctg tcctcgctgg
     2821 gcagcccctc ccggctggat ctgaccgtgg cgggcagcta cagtctgggc ggcgatttgt
     2881 cccgctcttt ttccagcggg acaagcctga ccgtcagctt taacagcgcc tccggccaac
     2941 tgacgctgtc ccagaatggc gtggtgatat acatgggcgg cgatttttcc ctgcgccgcc
     3001 acagcacctc cggcgtgaac ggactcaaga tcgcacaggc ccgggaaagc ggcaatccct
     3061 atcccggcga cctgtccttt caggcagtga agcagtccgg cgggagctat aagctctacg
     3121 tcatcgccca tgtctacatc gaggactatc tctacggcgt ggtgccctac gaaatgggca
     3181 actccagcag tgtagaggcg ctgaaggcac aggcggtggc cgcccgtacc tacaccgtgc
     3241 ggatgatgag ccgccgcgcg tccaaccgct acgacgtggt ggataccacc agcgatcagg
     3301 tctaccgggg cacgccctcg ggcaacgcca actgcgtcac cgccattgac tccactcggg
     3361 gtatcgtgct tcagtacggt ggcgagtacg tcatgaccta ttattccgcc agcaacggcg
     3421 ggcagaccga atccgcgccc catggcgtgg gcagcggggc atatgcctac tttaccgtca
     3481 aggacgaccc cttcgattac ggaaacccta gctccacggt gaaaaagaag accgtttaca
     3541 aggatctgac cgccgcgtcc aacccctccg ggctgatttc tctgctgggg cgcaaggcgg
     3601 cggctcagat ggggcagagc ggcgtcaccc cggtatccct tcagagcgtc acgccccata
     3661 cgcccaaata cgaggccccc agccgcctgt atacgaagat ggactttgag ctgaccgccc
     3721 gaaccgccag cggcagtctg caaaccgtta ccgtaacctg cgatattttt tccgagctgg
     3781 aaagcatgct gggcatgagc atccagtccg ccaaaaatga actttggagc gtggaggaga
     3841 cggcttccgg gttcagcctg caggcccggc gctacggcca cggcgtgggc atgagccagc
     3901 ggggcgcgat gtacatgggc aagctgggct atacctacga tgaaattttg ggcttctatt
     3961 acgagggctg ccagcgggtg cagaaaagct ttaccagcac cattctcagc gcctcctcca
     4021 gcgaggagga gaccaccgtc cgggaccccg ccgacnnnnn nnnnntgcaa gggtacggtg
     4081 aagctggtgt ccggcggttc gctgatcctg cgcagcggca agtatgattc ctcgtctgtg
     4141 gtcagcacgc tgagcaacgg caccatcgtg gaggtgctga cgaatgacgg cagctggtgc
     4201 tttgtgcgct acggcagcct gaagggctac gtgcccacgg aaaatctggt cattaccggc
     4261 accccccagg gttcggaaga agcagccacc acggtgcagg gcttcgtgac ggtcacggcc
     4321 aacgattatg tgaacctccg tcaggagccc agcttgagcg cgaaaaagct gggcacggcc
     4381 ccgaccggcg cggtgctgac cgtgttctcc ctttcggggg actgggccta tgtgcagtat
     4441 atgagcatca cggcctacgt ccaccgcaac tttatctctg ccctgaccac ggtctatccg
     4501 ggccagagca gctccggtgg cagtagcatg gccattgtga ccacccaaag cggcacggga
     4561 acggtgaatc tgcggacgac ggcctcgacg gtcggcgcgg ccattgccca actgccggtg
     4621 ggaagccggg tgacggtgct cagcgacgac ggctcctggt gccgcatcca gtggaacggg
     4681 ctggaaggat atatgatgcg ctccttcctt cgctatgagc aggacgcaca gccccccgaa
     4741 aacacccctt ctcccacgga atcggccgcg ccttcggaaa gcgcttcgcc ctccgaaagc
     4801 ccggccccca cgccccgtcc cgccggtgtt tccgccgcgg tggccagcca gctggtctac
     4861 ctgcggcagg agccgtcgga aaactccgcg tctgtggtgc tgctggcctt cggcgacccg
     4921 ctgacggtct attcccaggc cggggaatgg tggcaggtca gctgggaagg cagcatgggc
     4981 tatctgcccg cgtccgctgt ccgcctggaa acgtcctctg aaacgccggg ggattccaca
     5041 gctattcgca aggcccgggt gacgaccgtc tccggcggtc tgaatatgcg catggagggc
     5101 aagagcggca gtcccattct gacggtgatt ccgcgcaata cggtgatcga cgtgctttct
     5161 gtgggcaaca gctggtgtca ggtcagctac ggcggacgca ccggctatgt gatgactgtt
     5221 ttcctgacgc ttgaggaaag cacggtcacg ccgacaatgg cccccacggc ggcgccgacg
     5281 gtcgagccga cagtcgctcc gaccaatgaa cctgctgtat cgccctccat ttcgcctacg
     5341 gcgtcgcctg aaaaaacgcc ggaacctacg gcagttccca cacctgtccc cacagctgcc
     5401 gggcctcagt atgcgctggt gaccactgcc tccggcagtc tgaacctgcg cgcccagcct
     5461 tcggcaattg gagcgcttgt gactcaaatt ccccgttttg gcatggtgga ggtgctggaa
     5521 tacggctccg aatggtgccg cctgcgctat aacgcgctgg aaggatatgc gatgacccgt
     5581 tatctgacgc tggcttccgg cacggccacg gtggttccca cgcccagcgc tacgccggct
     5641 cccagcgcgg gcacggcccg ggtcaacacc gtctccggag ggctgaacct gcgtcagtcg
     5701 cccaaggcca gcgctaaagt gctgacggtc attccccgcc attcgctggt ggaggtgctg
     5761 gaaacgaccg gcagctggtc tcatgtgcgg tacggaacgt gggacgggta tgtggtttcc
     5821 agttacttgc ggtatgagac ggcgggttcc acaccgaccc cagtgccgac ggcaacgccc
     5881 gcctccggcg agacgaacgc gcctacgcca tcgcctgcac ccgtgaccgc cgcgccgact
     5941 gcgtccgtca ccgcaacgcc ctctcccagc cctgctcctg tactggacga tacgctggaa
     6001 gacgtgccga acggcattct ggcgctgatt gctccccggc agggagaaag ctatgtggcc
     6061 gtctggacgg aatgccggga acggggcgaa aagctggccg tgctgccgga aggctccatg
     6121 gcgcgtatca tacgccgggg cgaaacctgg tgcgaaattc agtatgatat gtacgatgca
     6181 tccaagaccg gctattgtct gacccaatgc ctgaatccgc tggagggaac atggaactga
     6241 aaccgggaga gagaatcgac gacctgcagc tggacgggct tcacatcctc caaaagaagg
     6301 atggcttccg cttcggtatg gacgccgtcc tgctggcgga tttcacccgt acccggcctc
     6361 gggaacgcat cgccgatctt ggcacgggta cgggcattct gtccctgctg ctgagtcatc
     6421 agcagcccga tacccagttt gaagccatcg agctgcagcc cgatatggcg gacatggccg
     6481 ggcgttcggt ggaattgaac ggcctttccg atcggattca cgtccacgcc atggatatgc
     6541 gtcaggcccc ggaagcgttc ggctacgaga aatttcacgc tgtggtgtgc aatccgccct
     6601 acggcaagca gaacggcacg ctgaaaagcc aaacggaaac ggtgtcgctg gcccggcatg
     6661 agcaggatac ctccattggt gagatcgtga aaagcgccgg ggcgcttctg cgcaccatgg
     6721 gacggctgac catggtgttc ccggcccagc gctttctgga actgtgcgac gagctgcgcc
     6781 gctgccgtct ggagcccaag cgggtgcgga tggtcnnnnn nnnnnacgcc aggcccggcc
     6841 tgcactggat gccgccgctg gtggtctacg atccgcaggg ccgggaaaca gcggaaatcg
     6901 aacgaatcta tcataagggc tgaactgcgc tcaacgcgtc tttctccgat tcatcggaag
     6961 aaaggcgcgt tttttgacgg aagaacagca cacccagatt ccacaaaagg gtgcagaact
     7021 gtcctgcccc ctgtgccagc agtacgccgt actgccgcag ctccggccgg gcgggcagaa
     7081 gccagatcaa caggagcgtc agcccggaaa cgggcagcga ccccagcatg gacagctttt
     7141 gcagccccag cgccgacaga atgccgccgg acacatgggc aaaggcgaac agcaggcaca
     7201 ggggcgcacc gaagcggaat agcgggg
//
LOCUS       DAWAEA010000025         7204 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_1671_length_7204_cov_2.141558, whole genome shotgun sequence.
ACCESSION   DAWAEA010000025 DAWAEA010000000
VERSION     DAWAEA010000025.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 7204)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7204)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7204
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1671_length_7204_cov_2.141558"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    900..909
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1281 a         2340 c         2017 g         1556 t
ORIGIN      
        1 catttccagg atcaggcgct gtgcgttcac ttcccgatcc agcttcatgc cttccgcaag
       61 gccgcgcagg taagcggtac gatccgtgat atttgccatg gtcttatcct ccaaatgaat
      121 tagcagcgct ccatatactc gccggtgcgg gtgtcgatgc ggatatggtc gccaatgttg
      181 atgaacaggg gcacctgcac gctgtagccg gtctcgacct tagcgggctt gtaggtgttg
      241 ctggcggtgt cgcccttgaa gccgggctcg gtgtcgatga cttccatctc cacgaagttg
      301 ggggcggcca cgctgaaggc gctgcccttg aagaacttca cggtcacgtt ggtgccttcc
      361 ttcacgtagg ggatagcgtc ttccacctga tccttgttca gcgggatctg ctcgtaggtt
      421 tcggtgtcca tgaagtagta cagttcgccg tcattgtaga cgtactgcat ttccttggtc
      481 tccacgatgg cgcggggcat tttgtcggtg gggttgaagc tgcgctccac caccgcgccg
      541 gtcatgatat tcttgatctt ggtgcgcaca aaggccgcgc ccttgccggg cttcacgtgc
      601 tggaaatcca cgatgttcca cacgccgccg tcccattcga tggtgatgcc ctttttgaaa
      661 tcgcccgcag taatcattgg tcattcctcc gttttttata tggtgtagaa ttggttgatg
      721 atacgccctg tgggcgaatc acaggataat gagttctctg gtggggctgg tcagggggat
      781 gcagccgtcc ttcttgacga tgacggagtc ctcgatgcgc acgccgccca caccgggcag
      841 atagatgccg ggttccacgg tcatgatctg gttttcttcc agaatggcgc tgcaggtcan
      901 nnnnnnnnng tcatgctcag gcggggttct tcgtggatgt cgatgcccac ggagtggccc
      961 agcccgtggc cgaagcgtcc ttcgtaaccg tggctgtaga tgtagtcccg ggcaatggcg
     1021 tcgatatcct tacaacattt gcctgccatg acagcgtcac gggcggtctg ctgggcggtc
     1081 agcacggtct ggtagacctt gcgcatctcg tcgctgggct gtcccagcgc caccgtgcgg
     1141 gtcatatccg cgcagtagcc gttgtacttc gcgccaaagt caaaggtaat catatcgccc
     1201 agacgaacct tgtagtcgcc gggcaccgcg tgaggcaggg aaccgttggc gcccgcagcc
     1261 acgatggtgg agaaggccag tcctgtcgcg ccatgggtca gcatgtcgaa ctccagcttc
     1321 agcgccagtt ccttttccgt cacgccctct ttgatttcgg gcagcagacg ttcgaaggct
     1381 tcgccggtga tgcggcaggc ctcggcgatg cggctcagct cgtcctcgtc cttgatctgg
     1441 cgaatgcgct gcacttcctc gtgcaggctc ttccattcca cgccgggcac ggccctgcgg
     1501 caggcttcga aagcgcggac ggtcaggtaa tcgtcctcat aatacagcgc gccggtctca
     1561 tgctctgcgc acagacggcc cacgatcgct tcatgagaaa cgcccttttc ggtcatttcc
     1621 accgaaaatc cgggagcctg cttttcggcc tgctccgtat agcggaagtc ggtgatgatg
     1681 gcctgcattt cccggccgat cagcaccaga ccctcgccgg tgtagccgga aagatagaac
     1741 atattgctgg gattatgcac cagcacggct tcgtgccggt ccagcgccag ccggtccatc
     1801 agtttcaaca ctctctgatt caacgataca tccctcgaat gcggtttagc cgcccagatt
     1861 ggcattaagc catttttcat tttaccataa acccaaaaac cgcgctacct ttttttgcat
     1921 tttagcgcgg tttttctgga tttttccaaa aacggcagag ttttttcgga tccgccggaa
     1981 gcatacgccc cattttcgac aggtcggagg cctttcttaa taccccaatg tttcctccac
     2041 aaacagcacg gtgatctttc ggccccgggg cgcgcggtcc accgccagcg tcaggcggca
     2101 catgctgtcg tgacagtccg ggccgcacat gccggtacgg gcgcaggcag tatccaaccc
     2161 ctgccgacgg gcgttgggcg ggcaggcggt tttcttgatg cgggcaatgg cggtattgat
     2221 gccgccgtcc accaccttgg aatggctgat gacgaaatag acctgctccg ggccgaaaca
     2281 cactgccgcc gcccggttgc ccgcgccgtc gatcagcacc agcttgccct gacgggtaac
     2341 ggcgttggcg gagcaaaggt acacgtccgc gccgatggcc ttgcggcgga tggggttgac
     2401 ttcttccttt ggcacgcccc aatgcgaata gacggtgcag cccttctcct gcagtttttc
     2461 cagaatgccg gtggcgcgca cgctgaccga tccgcctacg cccacagtct tcccggcggc
     2521 gatgtgttca ttcagataat cgcaggcttc ctgcgccgtg tgcagctcca ccacttcaaa
     2581 ctcatttttg cgcatgttgt caacaacctg ctgcagaaat tcatccatgg tccgtacctc
     2641 cccaatggct gggatgatgg ctcatccgct tccaaatcct tccctgaggg aatcggcttc
     2701 agtataacac aaatcatgtg aaaagggaat tttcacttcc gcccggtaaa tacgggaatg
     2761 gaaatgcgca catccaccgc ggcgcggccc gtgttcaccc gcatatcggc ccgaaggccc
     2821 gcgtcctgca tctgctccac gatctcgcgg atggcgttca ggtacagccg ataatcccgc
     2881 atcagcggct tcatatggcg gccggaggtc atgggcaggg gcatccgggc cagttcgccg
     2941 tctaccagcg tttccgtgcc cttggtgtcc agccccagct ccaccacgtg ccgcagcact
     3001 cgaagccgtc cctgctggcc gggcatccgc agcagcgcct cgccgatgga ttcgctcagg
     3061 tcatagtgcc gcagcagggc acagacctcc tcccccagat tgagcaggcg aatcttccgc
     3121 cgcaccgtct gcacggaacg gcctgtccgc tgggacagct gctgggtggt caggccgtcc
     3181 tgacggatga agctgcggta ctgctccgcc tcgtccagat aatggagccg ccccttcgcc
     3241 agcctgtcca cgaaagcgga gagccgttct tccagcggaa ccccgggact gatcaccgcc
     3301 gccacatagc agctgccttc ctcccgaagc cgctgcagct gctcctgtcc cgccaccaat
     3361 gtatagcgtc cgtccgcctc agcctccacc tgaatggccc cggccttggc gctgctttcc
     3421 gcccggttcg tttcctgaat gtcgctgaga ggtacccagc ggatctgttc ggctgtcggc
     3481 tgcatcccgt tcgccccttc ctcacgaaaa aatgaacgcg ccccgcgatc cccgcaggcc
     3541 ctcccccatg acctgcgaag cccacggaac gcgtccatgc ctgtgtattt ccctgcacag
     3601 gcggcttttc ctgccgagga aaggcgggaa tcgttcccca catttcgaca gcggcgggca
     3661 gtaaaaaaga gaccctgacg cgcagagtct ctcttggttt atgtttcttt tgtgccgttg
     3721 ccgcgccctt gagccgggaa cggcgaccgt gcaagggttc aggctttccg gcttcttttt
     3781 attttccgcc ggtctgtccc aacggctccc ggcccggcgt gccggctttt cggggatact
     3841 gtcgaacggt tttttccttt ttaggcaggg cgatgacgca atgctcccag tcctcctgcc
     3901 ccggcagcac gatgggcgtt cgttccagct tgccaccgcc cagcagacgg gcggcccgct
     3961 gtcccgcgtc ccattcttcc gacgcggcgg ggcctttata gcaaacagcc tttccgccca
     4021 ccttcacaaa gggcagcagc agctccgcca gcaccggcag cgccgctacg ccgcgagcca
     4081 ctgccagatc gaactgctcc cgccagcggg gatcccgccc gccgtcttcc gcccgggcat
     4141 ggcacagccg cacgttggaa aggcccagtt ccttcaccac cgcatccaaa aaattcagac
     4201 gcttttgcag cgaatccatc agcaccacat ccaggtccgg ccgggcgatg gccagcggca
     4261 agccgggaaa acccgcgcca gtgcccacgt cgatcaggct cgcgctttcg gggaacagtc
     4321 cctcgatgcc cagcggagcc agcgaatcca gatagtgccg cccgagagag gtctcaaaat
     4381 ccgtatcgcc cgtcaggttc atgacctgat tccactcggt caacagctgg tggtagcggg
     4441 ccaatcgttc cggggtggcg gggtcgtaac ggatgttcag cgcgtccagc ccgtttttca
     4501 gccgttccgg ccactgctcc cggctcacag cttcagtccc agataccgca cggcccaata
     4561 cttcccccat gccagcacct ccctgagatg attcttcaga aaatacttca cgcccgggcg
     4621 gcaggggctt cccacgccct gcgcgtccat gcccatatcc tgcatcagcc gcagagcccg
     4681 gggcagatga taatcactgg tgaccaccac cgggcggctg cacccaagct cctgcaaaat
     4741 ggcccaggca ttctgcagat tctgccgggt gtcggcgctc aaggggtcgc tgatgacgtg
     4801 atcctcctgc acgccctctt cgataaacag gcggcgcatc acgtcgccct cggcttccgg
     4861 ctccttcccg gccttgccgc cggtgaccac catatagcag ggatgctcgt cgtatacctt
     4921 ccgggccgcg tccatgcgcc agcgcagctg caccgacggt tccccgctgg gcaatacctg
     4981 tgctcccagc acgataatgc tgtcataatc cctgggctgc ggcacgctga cctcctgcgc
     5041 gtagatccag cccaccatcc cggcgtagca cactacgccc agaagcagca gaccgatcag
     5101 aattttccag aatgttctcc atggttttgc gcttttcttt cgcgtcatgc tttcagtctt
     5161 ccgtcctttt ttcggatctt atcagcaatg cgggtctctc ccgcagagct ggccaccttg
     5221 atgaccggct ctccgttgcg gtactcccgc tggatcagtt cgctggcgta caggctgaag
     5281 gtttccccgt ccgccacgat gatatgatcc gccagtgcca cttccagact agaaagcagc
     5341 gccgccagct ccgccgtcat gtccagatcg ctctgggagg gcacggcgct gccgccggga
     5401 tgattgtggc acagcaccac cgaatgggcg ttgtgccgca gcacggcctc cgctaccagc
     5461 cggggatagg cggctacctc gcttaaagag ccggtagcga tctgtacgtc ggccaatacc
     5521 tgcagctggc cgtccagaca aaccacgtaa aacgcttcct gacggcggcc cgccagcagt
     5581 ctgcggcaat gttcctgcgc ctccatgcgg ttgcggagct tttcccgctg cccggcgcgg
     5641 ctttcctcca cccggcggcc cacggcggca aacagatgaa gcagcacggc ggcgtactcg
     5701 cctacgccct ccaccctgca cagctcctcc tccgaggctt ccagcacccc gtggaagctg
     5761 ccgaagcgtt ccagcagtgc gtgagccagc ggattgacgt ttcgctgggg aatggcgtaa
     5821 aacagcatca gctccagcgc ctcatggtcg gcaaagccgt tcagcccttc cctgcggaac
     5881 cgttcccgca tccggccgcg gtgcccgtcg tgcgctcccg ccatggtctt ccctccccgc
     5941 gtttgtgtcc attgtgttag tccagtatac cataaacaag gaaagcttga aaaccccccg
     6001 ggtttgtgct agtatcagac tggaattgtt tctattgaag gaggctcggc catgaagatc
     6061 tatgaatccg gcgaggatta tctggagatg atcctgatgc tgcagaaaaa aaagggttat
     6121 gcccgctcca tcgacgtggc cgcagcgctg ggcgttacca agccgtccgt cagcgtggcc
     6181 atgaagaagc tgcgggaaaa cggctacatc ctgatggatg gcgacaatct gctgtccctg
     6241 accccggcgg gcgaagccat tgccgaccgc atttacctgc gccacactgt tctgacccag
     6301 atgtttgtgg cgctgggcgt ttcggaagag acggcccgtc aggacgcatg caagatcgag
     6361 cacgacctgt ccgacgagac ctttgaggcc atccgccggc atctggaaaa catcgaaagc
     6421 caaaaataag gagtttattc caatgaagca acggcgtctt tctccaaacg atggcggcaa
     6481 cggcatcacc gaaggcgtga tctggaaaca gctgcttctt ttcttctttc ccattctgct
     6541 gggcacgttc ttccagcagc tgtacaacac cgccgatgcg atcatcgtcg gtaaattcgt
     6601 gggcaaggaa gccctggccg ccgtgggcgg cgccaccggc aatctgatca atctgctggt
     6661 gggttttttc gtggggcttt cctccggcgc aagcgtcatc atctcccagt attacggggc
     6721 gcggaaaaac gccgaggtca gcgcgtccgt gcatacggcg gcggcgctgg cgctggcggg
     6781 cggtcttttg atgaccgtac tcagttattt cctgctgccc ggctttctgg tcaccatggg
     6841 caccccggtg gagatcctcg atcaggcgtc cacctacacc cgcattattt cgctgggaat
     6901 gctgccctcg ctgatctata acatcggttc cggcattctg cgcgccatcg gcgactcccg
     6961 ccgtcccctg tatttcctga tcgcctgctg tctgttcaac atcgtggccg acgtcctgct
     7021 ggtagtgggc gcgaatatgg gcgtggcggg tgcggcgctg gcgaccatcc tgtcccagtt
     7081 gctcagcgcg gtgctggtga tcgtcaccct gtgccgcact cagcagtcct atcagctgcg
     7141 tccccgggaa atccgcttcc atggtgcaat tcttcagcgc attttgcaga ttggtttccc
     7201 agcc
//
LOCUS       DAWAEA010000026         7153 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_1684_length_7153_cov_3.049310, whole genome shotgun sequence.
ACCESSION   DAWAEA010000026 DAWAEA010000000
VERSION     DAWAEA010000026.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 7153)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7153)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7153
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1684_length_7153_cov_3.049310"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6808..6817
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1366 a         2387 c         2052 g         1338 t
ORIGIN      
        1 cacgatgacc ggcgccgcgt ccgcccagat caggctggca tcgttcatgt ccgtatgggg
       61 gaagatgcgc tccccggcgt agcttgcggc ggagaaaccg aactcaccgt aaatctgttc
      121 caactcatcc gcggtgatat agtagcgcgt cctgctgccg aaggccatct gctggacggt
      181 ttcttccacc gtgaccagct tccattcgcc actgaccagc gcatagcact ccacggtcgc
      241 agtattcgcc gcaggagccg catcgtccgc aagcgcctgc accgcgccga ataacatcac
      301 catgcaaaaa ataaaggcca gaatcatctt cataaaaaga tttttctgac acttacgctt
      361 cttccctatg gaagcgttcg ctctctttcc acctgcaaag caagcctcat cgcctgctta
      421 ccagaacatc tcctcgcctg tcagggatga ggcctgacaa attcttagcg atatcttagc
      481 aaaaatgata ccatatcgat atatgaaatt caacttgcgt accacccctt ccatagggcc
      541 gaaaaaaata aaaatacaat ctgccggcaa aaataaccgg cagattgtat tttatgactg
      601 ctcgtcctct tattcgcccg ccagtttttc gatggcatgg gcgatgatgc gcacgttctt
      661 catcatgctg tccagcgtga tgtactcgtt ggcctgatgg gccagttcct cctcgccggg
      721 gaacacgcaa ccgaaggcca cgccctcgtc cagacacttg gcataggtgc ctccgccggt
      781 ggacaggcac tcacggggca gacccgtcac ctcgtgataa gcgtccagca ggcactggat
      841 cagcttagaa ttgggggcca catgatgggg ctccttctgg ctgtcgatgg tggcggtcag
      901 gccgtccgac acggcggcgg tcagggcggc gctcagggcc ttgtggtcgc acagaatggg
      961 gtagcggatg tccagcgtgg cgtacagtcc gttttcctca tcgtaatgca gaatgcccag
     1021 gttgcaggtc agggggccgc tggtttcgtc ctgacagcgg atgcccaggc tcagaccgtc
     1081 gtattccatg cccacggtat cggccagcgt gcgcagaccg ccggtcacgc ccagctcctt
     1141 caaaatcagc agcagctcgc ccagcgcgtt cttcgccgct tcggggtagg ccgcgtggcc
     1201 cgtcgcgccc aacgtgtgca gccggacggt atcgccattc acagccgcat ccgcctcgaa
     1261 gcccagcttc ttcgccgctt ccagcgcctt agcggccagt tcttcgccgc cgttcagctc
     1321 ggcgctggct tcgccgggca ccacgttgtg ccgttcgccc actcgaatgg agcgcaccag
     1381 cccgttcttt tcatagggag accgcaggct caccagcagc attccctttt cggtgttgat
     1441 gatgggatag ctggcgtcgg ggctaaagcc cgtgcggggc atatcgcagt gggccttgta
     1501 gtaggccatg tcgtcccacg aggtctcctc gctgcagccc aggatcagcc ggacttcccg
     1561 gttcagggga atgcccgctt ccttcacggc cagcatggcc agcagcgccg ccacggcggg
     1621 gcccttgtcg tccccggagc cgcgggcgta caggcggccg tccttcacca ccggctcaaa
     1681 gggatcggtg tcccagccgt ccccggcggg caccacgtcc agatgggcca gaatgcccag
     1741 cggatcctgc ccctccgggc ccatgcgcac gtcgccggca tagccgtcga agtcccgcac
     1801 atcaaagccc atggccttcg cgtcgttcag ggccacggtc agcattttgc gggtctcctc
     1861 gccgaaaggc gcgccgggga tttcctccgc ccggacgctg ggaatgcgca gccagcgatc
     1921 cagcagttcg ttcattttgt cctgcagctg gtcaatgatc tggttcaggt ccttcataaa
     1981 aatcttcctc cgtttccgca gtgatccaaa aatcaggctt tcagcgcttt ttccagccgc
     2041 ttgacggctt ccagcgtctg gctgtggggg caggcgaagt tgaagcgcag gaagccctcg
     2101 cccgcttcgc cgccgaagcg ggtgccctcg ctgaaggcaa cgcccgcctt gtgggtgcgc
     2161 tccgtcagct gcgccgtggt aaagccgtag gcccgcagat ccagccagcc cagataggtg
     2221 gcattcatgg gggccagcac cgcttcgggc agccgggcgt tcagttcccg ccgcagcagg
     2281 ttctccgcct cccgcaggta gtccagcatt ccgtccagcc acgggtcgca ctggccatag
     2341 gccacctctg tggcggtcat ggcgaaaata ttgccctgcg tcacgcccgt gctgtgcatc
     2401 tgctcctcga cccgcttttt cagctcctga ttgcgggtca gcagcaccgc ctgctgcaat
     2461 ccggccagat tgaaggtctt gctggcgctg gtcatggaca gcagccgggc gttttcgtcc
     2521 tgcacgatgc gcaggaaggg cgtgaccttg cctttcagct caaagtcctc atgaatttca
     2581 tcggagacca gccacacgcc gtagcgttcc agcacgttcc acagggcgcg gagctcgtcc
     2641 tccgaccacg cgtaccccac ggggttgtgg gggttgcaca gcagcatcac cttggccccg
     2701 gccttgcagg cggactcaat cgcgtccaga tccatgcggt agccgccgtc cggctgacgg
     2761 atcagcgggc agtccgccag ctggcggccg ccgtcccgga tgctggcgta gaaggggccg
     2821 tagaccggcg actgcacgat caccttgtcg ccgggttcgg tcagggccag caccgacgcc
     2881 cgcattcccg agaccacgca gggcagcatg agctgctgct ccgccgtcac cgtcaggccg
     2941 tggcggcgct gaaagaagcc cagcatggcc tcaacagccg cgtcgctctg ctcggtgtag
     3001 ccgtacaccg ggtgagccac ccgggcggtc agcgcctgaa cgatctcgtc cgcgcagcgg
     3061 aagtccatat ccgccaccca catgggcaga aggtcgcttc cctcccggcg gcgcagcgcg
     3121 tcccactttt cacagttggt tcccatccga tccaccggcg tgtcaaaaaa agaacgatcg
     3181 tacttcatgt cccagtttct cctttgtttt tctaatggat actattcctc gtcgggccgt
     3241 tccgcgggct gcgtgcagcg cagcacccag aagccgcctt ttttggtcgc ggcctccacc
     3301 cggccaaaca gtgtcttcag atagcggacg gcggactccg caccctgctg cttgcggatc
     3361 accagccaca gctcgccact tggttccaga cggcgggctc cctcggcaaa catggcgtag
     3421 atcacgcttt tgcccgcccg gataggggga ttctgcacga tccagtccat ccgctgaaag
     3481 ggaacgtccg cgtagccgtc gctctgcatc acccgggccg gtacgccgtt gcgctgggcg
     3541 ttgcggcggc tcaattccac cgcccgctcg tttacgtcgc tcatcacgac cgacaggccc
     3601 ggatagcgct tgcccagcgt cacgccgatc acgccgtagc cgcagcccat gtccagcaga
     3661 ctgccggtca gctcttccgg cagggcgttt agcaaaatct ccgtcccctt gtcgatctcc
     3721 gtgcgggaaa aaacgccgct gtccgtttca aagacgagct tcgccccccg ccagaggaag
     3781 tccacctgcc ccggcttgtg ggcgctggtg ggcgtttggg tataataatg gtcaggcatc
     3841 ttcgttcctt tccggctcgg tcagccccgc ttcttcccgc agggcggaca gttcttcctc
     3901 ggtcagctcc cggtactgcc ccggcggcag ctccgcaggc agccgaagcg caccgaaggt
     3961 caggcggctc aaggccgtca cttcgtgccc cagcgccccg aacatccgct tgatctggtg
     4021 aaacttgccc tcccgcacct gcacgcgggc gcggctttcg tcctccccgg cagagaggat
     4081 ctccatccgg gcgggcagag cggtgaagtc cgacagggta atacctcggg cgaaggcctc
     4141 ttcatcggca gcggtcagcc gccccgtcac cgtggcctcg taggttttcc acacgtgacg
     4201 ctttgggctc agcagccggt gagccagctc gccgtcgttg gtaaaaagca gcagccccgt
     4261 ggtgtccttg tccagccgcc ccacgggcag caccttgcgc cgggcgcagc tttcgggcaa
     4321 cagctgcatc accgtggggg cccggctgtc ccgagcggcg gtcaacagcc cggcgggctt
     4381 gtacagcatc aggtattggt aacgcttctc ctccacggcc tgtcccatca gctccacccg
     4441 gtcgtcgggc ttcagatgaa agccgccgtc ccggaccggc tcgccgttga cccggaccgc
     4501 gccggaacgc agcagccgcg ctgcttcgct gcgggttcgt tccccgctct gggccagata
     4561 acgatccagc cgcatggctt acgcgtccgc gcttcggtcg gaagcgccgt tttcctcatt
     4621 ttccgtccgc acccagtccg gcacgatggc cttgggcagg gtagggtctt cctccaccgg
     4681 ctgtccgctg cggggggcgg tgtgacgggg ttcccggacg gcaaaggcgg cggtcaggat
     4741 gcgccgcgcc ggaagcagcg gcatataggt caaaaagaag gtcagcgtca gcggcagcag
     4801 ggtcttgacc acatcggggc tgatctgcgg ccggccgttc cacgacatgg ccagcaccag
     4861 ctgctggcac agctccagaa aggtatcctt caactggaga gcggcgagcc acaggaaggg
     4921 aacacacagc agtagattgc acagcgccgt gcccagctgc gccatgcggc ggccccgcag
     4981 gcggcggcga atctcccggc gcaccgggcg gtcttcatgg ttggccacgg cccaaatata
     5041 gcgggtggcg ctgttgcgca ccgcgccgta cagcaggatc agcagcccca gccccaccag
     5101 cgccagcacg gtatacacgc cctgcatata ggagccctcc accgtttcca gcggcagatg
     5161 gccgaacagt ccccggaaga aattcaccac cgcgtaccac gtgccggaga cgttcttgcc
     5221 cagctcccgc aggccgtcca gcaggccctt gacggtgtac tgttccttca aataataacc
     5281 ccaggcgccc agcccggcca gcgggatggc ccatttgagc acgctcaaag catggcgcag
     5341 gctttccgcc agcttttcgc cgtactggcg aaggctcagc gcccggtcga tggaaaaatc
     5401 ccgggtacga ggccgctgca ccagcgcgtc cgcgaaggaa aaccgcagcg gaagcaccag
     5461 aaaaaccacc atcagtgggg aaatcaccca cagccaccgc cagagggaat ccgccgggaa
     5521 aacaaacagc gccgccagcg gagccagcgc cgccgcccgg atcagcagtg cgacgaggca
     5581 atacagcagc acgcgcacat aaaacagaaa tacgttttgg tttttttgca tcaggcagcc
     5641 atccttcctt gccttgggag ccgcttccaa aagccgaagc ggctcagggg ggatcgggac
     5701 acaaagtccc gtatcatata ttataaagca tcctccgggg gcttgcaagc ccggaggatg
     5761 cggcggattt ttctccgccg gtttatccat tctttttctg tccggggcga aaaagaaaat
     5821 ttatcgcaaa cgtttgtctt attctttttg ggtcacttta caccgatttt acaatgtggt
     5881 ataatagggg caccattcgg aaccgagaaa aagttccctg gaaaaataaa ggaggaacat
     5941 ccataccatg aaaaagactt tgtctatcgt tctggcgttg gtcatggtgc tgagcctgtg
     6001 cagctttgct tccgcggaag actactccgg ctataccatc cgcatctact ccaactccaa
     6061 ctccaccgag cgcaccaact ggctgatcaa cgaggccaag aacgccggct tcaccatcag
     6121 cattgacgac aactccgtca tctccggcga caccgccgcc attcaggccg ccaatgagaa
     6181 caaggacggc gacatcctgt tcggcctgaa cgagacccgc tggtctcagg tcatcaacgg
     6241 cacctacgag aacctgtcca tcatggactg gaaccccgac tgggccgaag aagtgggcga
     6301 atacaagtat gacggcaagg cctacggtct ggtcatccag aacgtgctga tgctgtaccg
     6361 caacgacgaa ctgggcacca acggcaccga gctgcacttc gaccactggg ccgacgtggt
     6421 gaactccggc tacacctggt accgtcagaa caaggtcggc ggcaccacca acgccaacat
     6481 caacagcgcc atgctgtacc ccttcaccga tcccaccagc cctgccggcg gcatcaccgt
     6541 ggaaggctgg aagaccctgt gggcctactg cgccggcggc aagagcggcg gcgatgacta
     6601 caagtacggc ttcacgcccc tgaaccgcgg cgacgtgcag gtttcttcct tctactcttc
     6661 ttctctgtat ggcaagatcg acgaggccgc ctctgaaagc gatcatcccc tgaagggcac
     6721 catggagcct gaaaactggg cgctggtgga catcgccgac ggcacctact acatcgccga
     6781 gtatctgggc attctgaacc gcgaaggnnn nnnnnnncga ggaagaaacc aaggctgtga
     6841 ccgctttcgc cgactggttc ggctccgcgg aagtgcaggc tgcctggagc gacgagttcg
     6901 actccttccc ctgcaaccag gttgccgcca aggaaatcta cggcgaagac atccccgcca
     6961 tctaccagat caagaacttc gccctgacca aggtcgaagg caccgacatg acctacgccg
     7021 agtatgtggc cgctcactcc gccgagtgga ccaacatcat gaccaacctc ggcttctact
     7081 gggccgacgc ttctcaggct cccgccgaac ccgactggga taacctgcag tgggacactc
     7141 tgacccagaa ggc
//
LOCUS       DAWAEA010000027         7042 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_1717_length_7042_cov_2.945900, whole genome shotgun sequence.
ACCESSION   DAWAEA010000027 DAWAEA010000000
VERSION     DAWAEA010000027.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 7042)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7042)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7042
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1717_length_7042_cov_2.945900"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6096..6166
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6623..6632
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1437 a         2124 c         2011 g         1389 t
ORIGIN      
        1 cgacccgcat atttccctac gcccctgcgc gccttaggcg cgcagtccga aagggggctc
       61 agcccctcag gggcactgcc cctgcctgac gggtctcgtt gtagtatctg acgatgtccc
      121 ggaggagctg gaagtgctcg aagtcctgcg tgtagacgat gttgatctcc cgcatcatgc
      181 tcaggttttc gatgggcagc accacgatct tcttcttttt cagctcgtcc atgcaggcgc
      241 tcttcgccag tacggaaacg ccgaagtccc ggcggatcag atccttgatg gtggcgatgt
      301 tgtcgatctc cagaaccacg ttgaaatcgt cgatgctcag gttctggctt tccagactgg
      361 ccacgaagag gttgcgggtg ccggagtccg gcagacggag gatcatcttc tccttctgta
      421 actcgccgat ggtcaccatg ccccggtggg ccagccggtg ccccggagag accgccagca
      481 ccagacagtc cgtgtccaaa atcaggtatt tcagactggg gtcgttgatc ttgccctcta
      541 cgatggcgaa atccagctcg taattcttga gcatgtcgtg cagattgctt tgagaatccg
      601 tgatgatctt gatggtcacg ccgctgcggc tgttcacata ccgggccagc gcttcggcaa
      661 tggcgttgct ctccgccgtg tgggtgatgc ccacggtcag ggaggtcagc tggcgctttt
      721 cactggtcag atccttgcgc agattgttgt agagggacag catccgccgg gcgtacttga
      781 tgaccatctc gccctcgtgg gtcacgtgca gctcgttgtt cttccgctca aaaatgcgca
      841 cgttcagttc gctctccagc gagcggatgt gctggctcac ggcgggctgg gtcagggata
      901 gctgttccgc cgcccgggta aagctccctg tttccacgac cttcatcagg gaatgaagct
      961 tcggatcgac catccctttc cccacccttt caaaaaattt ttatgaatga tgattatacc
     1021 ataaacttta ggaatgtgcc agcttcgtcc agcgccggcg cacaggctcc gccgtggcgg
     1081 gcttgtcgtc cagcagaccc tcctgccccc gcaggaaagc gacgaacatc gcgatggaga
     1141 tgcagcagca gatcacgtcg gtgatggggg tggcccagac gacgccgaag atgggcagaa
     1201 gccggttgag caggaacatc agcggaatgt ccagcagacc cttgcggagg atggccagca
     1261 caaaggagcg cattcccttg cccaccgcct gaaagaagga gatcagcgca taggcgcagg
     1321 cggagaaggg cccgcccaga cagaggatcc gcaggaaggc cgcgccgtag gtcacggact
     1381 cgctgccgcc ggggatgaac aggccgatca gctggcggct gaacaccagc gagacggtca
     1441 tgcacagggc cgccatggcg atagagagcg ccatggatag ggtaatggag gccttcatgc
     1501 gcttgtgatc gccggaggcg tagttgtagc cgatgagggg cagcacgccc tgcgacatac
     1561 cgcggacgat gctgtgggcc agcatattga ccttcttcgc cacgcccaga ccggcctgca
     1621 gggccgtgcc ggagaccgcc atcagcgcgt ccagcaccgc gtaggaaacg ttctcgcaca
     1681 gagtcatcag gcaggcgggc agaccggtgg tcagcacgtc ggcggggatc cggctgcgga
     1741 gggacttgcc cagacggagg gggctcagcc gcaggatgga gcgcttgccc tccctaagca
     1801 ccaccacaag gaagtacccc agcgcgatga cgttggacag ggccgtggcg atggcggcgc
     1861 ccagcgtctc atagccccgg ggcaggagga cgaacatgaa caggggatcc agcgcgatgt
     1921 tcatcacgcc gcccagcgcc acgccgatgc tggcctgcag ggaacggccc tcggagcgaa
     1981 ccagatgggc cagcagcgcg ttcagggagg tggccagacc gccgatgacc acggtgcagg
     2041 tcaggtactc caccgcgtag tcatgcacca gcggattgga accgcccagc aggtctacaa
     2101 agggcccggc gaagagccac gcgcccagcg aatacagcgc cgtcagcgct gcgcagcccc
     2161 agaaggcgaa ggaggccgta tccttggccc gctgcaggtc ccgcgcgccc agcgcccggg
     2221 agatgacgct ggctccgccc acgccgaaca ggttggagat ggcctgcagg aacataaagg
     2281 cgggcataca gaccgtggcg gcagtgatca tggcgtcgct gccggtcagg ccgataaaga
     2341 acgtatcggc catgttgtac aaaaccagaa tgatctggcc gaccaccgtg ggcacggcca
     2401 gcttgaagat cgcctccagc acaggcacgt cttcaaacaa cgtcttttct ctttccttca
     2461 taaccgtggt ccctccgttt ctaaaaaaat cagcggcggc cgcgcaaata tgccgcagcc
     2521 gccgcagaag cgtccgtgcc gtccgtctat gcgacggaac gacgtcttcg cctctgctgc
     2581 cgggacaagt tcatctgcga tttggaaaaa gatggtttgc tattcatccc ggcacgtcct
     2641 ttcctgtggt ttttggagaa gcctttcatg gccgttcccc ttgggatagg catattatat
     2701 gggtctttct ccaaaatatc caataatcat tccgtattta tcgataagaa atgcttatga
     2761 agaggaatgg acaaaagaac aaaagcgtct ccgcgcactg cggaaacgcc gggttcatgg
     2821 gctttgaagt cccttctgtg aaagttttat caatcttttg ccaaaatgga cgcaaacaaa
     2881 ccttaatgac cccaaaagtt ttcttatccg aggccccaaa ggcattctgc gattcaggcg
     2941 cgtccctttc cgaaaaaagc cgttttccgc ccaatgaaag cagaaaatgg catgagctta
     3001 tttccgttca ttcatcctgc ccacagtgca ggttccattc ttcttcctcg caggccagcg
     3061 gcagcagcac cgtgacggca gtgccctcgc ccagccggga ctcgatacgc agctctgccg
     3121 cgctgccgta gatcagccgc agccgatcac ggacgttccg catcccaatg cccgtagact
     3181 tgcggccctg ctgttcgctt tcctccgccg aaccattcag aatacgctcc acccgctccg
     3241 aaggcattcc ctcgccgttg tccttcacgc agattaccag ccggtcgccc tcgtcccgag
     3301 cagagatatc caccacagcg tgatcggtgc gcacgccgtg aaccagagcg ttttccagca
     3361 cgggctgcaa tatcatggtg gggatcaggg caaaggccgc gccggtggtg cagtgaaagg
     3421 gcgtttccaa attttcgccg tagatatgcc ggtaaatata ggcatactgc ttcagactgt
     3481 cgaactcccg cttgacggga ataaagcgct tctgcatatt gccggtgttc tgtcggaaaa
     3541 aagacgaaat atgctgcaca acttcgcaga tctccgcgtt gccgtcgatc ttggccagag
     3601 cgttgaccat ttccatggcg ttgtagatga aatgaggatt gatctgggat tgcaacgagc
     3661 gatacttgta ttccaacatt tcatactccg cccggttctt gcgggtctcc tcctcccgca
     3721 cctgcgccaa cagccgctgc tgatgctcca gcattctgtt gaagtggctg gccagtttgc
     3781 ctgtttcatc gcggctgacc accggtaccc gaagctccag atttccggcg gccgcctcgt
     3841 ccaccgaact caccagcaga tggatctgcc gagtcatgcg gtcggtgacc atcagtacta
     3901 ccagcgaacc aagcaccgcg ccgaccaggc cggtcaggat gccggtcagc gccgccgtac
     3961 gccgcacctg ccgcaggatg gattccggca gcaggcccac cgccgtccag tcgccctgct
     4021 tctggatgga aacatagtat ttctccccgg cggaaagctc cgccgcgccg gttcgagccg
     4081 cttcctccgc ggcccgtgct atgccttctc ccgcagcgcc gctgacggtc agggtctggc
     4141 cgtcggccga taaaaacgcc acggcgcagt gcaggttgtc gttgtaggtg cccagactgc
     4201 ccagttcttc ctgcttcacc cgggccacca gcttgcccat atgacgaaag ggcgcggggt
     4261 tgtacacatc ccggatcaga tagacctgcc cggtctccga tgtgcgccac tggctgcggc
     4321 cataggccgt gccccgcagc tcctcccgct ccatcagcgc cgccgcctcc tgacggaacc
     4381 ctgcggaggc ataggaagtg gtgaagctgt ttccccgatc cagccgcaga tacaccgcgt
     4441 catagccgga ctgggtggtg atggtctgat tcagggcata caaaagagac tgggtagcat
     4501 ggtaataccc ggcggaatcg gcggtcatat cccgcatggc gtacagctgg gcgctcaggc
     4561 cagtggaatt gacctgattg ttcagctgcc gggccaccgc ctcggtctgg ttgatgaaat
     4621 tgatgagcac ctgctcggtc aaaagctcca gatgggcctt ttgatcttcc agcatctgcc
     4681 gggtcgcgtt gtacgtcagc cccagcgaca ccatgcccgt agccagcaga atgaccacgc
     4741 accagatcag gatcagcttg acccggtaac tgaagacgcc gatttttttc atagcttggc
     4801 ctttcggttg cgatattcgg tgggtgtcat gtgctcgatg cgcttaaagg cattgtagaa
     4861 ggaaatatag ttgccgtacc ccaccgccct ggctacttcc tccaccagga tccgggggtc
     4921 agccagcatg gaacgggcca cgttcatacg cgcttccagc atatgctcgt gaaaggtcac
     4981 gccggtctcc ttgcggatca gggcggagag atagttggcg ctgacgcaca gcttatccgc
     5041 cacgtcgttg agctgaatgc cctcgatggc gtgcagattg atatattgca gagcattgcg
     5101 ggtcaaaagg gattgtttgg gcatatccat ccgccggagg aaagcctcgt tcagctcctt
     5161 caggcacaga cgcacatcct cctccgtgct gacgaaccag atgccgctca tcaccgcgtc
     5221 cagctcctcg tctgcgctca ggccgaaccg gcggcgcagg cctgtagtgt acagaaacat
     5281 catagcccgc aaattgctgt actggcggcc gctctgctcg gccaatgccc gggccgcttc
     5341 cgcggcaaaa ccgtccgtca gctccgccct ggcgatcagc tcatcgatct gccgctgggt
     5401 gtcggcgata tgctcctgcc ccactgggcc gtcgccctga tcctcgtaga aaacgcaggc
     5461 gcggtttccc ttgcgcaggg ccgcctccca cagggcgcgc ctggcctgca gataggcctg
     5521 acggatggcg tggctgctct gccccagccc gctgacgccg atacggacag ggttgaccag
     5581 ctcgtcaaag aggtcgtagg ccagttccgt ggcttcctcg tgccagccct tgccgtcgtc
     5641 cccccggggg acgaagatca ccgccagatc gtatagcagc agcgtgaccg tccgcatccg
     5701 gcgacgggcc agtaccgcct ccacccggtt gagcacagcc tgagaaaaat agcgctcctc
     5761 cgtctgctgc agcgccataa tgtagtaggt gtgaaattgc agccccgccc cggccagcag
     5821 ctcatgaacg ccctgtcccc gctgagaatc attggtcaaa agggtcagca gctgggcgca
     5881 cattttcagg ttttcggcct gttccagcgc cacatcgttt tccatctggc ggcgggtcac
     5941 ggctgcggcc cggcggacgg tgcgcatgac ttcttcattg tcgatgggct tgagcaaata
     6001 atcgaacacg cccagcttga tggcctgact ggcgtactga aactcatcgt aaccggtaat
     6061 gatgacaaag cggcagtccg gcacatcctc ccgcannnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcag cacgatatcc
     6181 ggcttcagct ggcggatcat cacgccgccg ctttccccgt cccccgcagt gccggccact
     6241 tcacacttca tcacgtccca gtcgatggtc ttcaccagcg acagcaccgt ggtagggtta
     6301 tcctcaacga ttacgacttt caggctcata gcggctcctt ccttatgggg gatgaacgaa
     6361 cgatcaagat ggactcatta taccaaaaag tgcccgaaac ggcaatgaaa gcagattttc
     6421 cccttcacaa aaaacagggc gggtctttcg gcccgccccg cagggtacag gaagtacggc
     6481 gttatccctt cacagcgccg gacataatac cggacacgat gaagcgctgg gtgctcaggt
     6541 acaaaaccag aatgggcagc atggccagca tgaagaaggc gaaggccaga ttcagttcag
     6601 tcatgttctc accgaaatag atnnnnnnnn nngaatttgc cgcagccgtc gataccggcg
     6661 gcttcttcca gatcccgggg gatgcccttt acgaagccgg tgatggtcag gacgctgatg
     6721 gagacctgaa agccggttcg ggccagaatg aagcccagat tggtgctgac cagtcccaga
     6781 ttatagatat tcatatacag aggaagcatg atgcactgga agggcaccag aatgccgctg
     6841 atgaacaggg aatagagcac cttatagaac ttggaatggt tccgagccaa cacgtaggag
     6901 gccatggagg tggtgaacag caggatcagc gtgcagggta cagtggtcag gatgctgttt
     6961 ttaaaaccga tcgccacata cttgttttcc cggatcacac gggcgtaatt ttccagcgtg
     7021 gggtgttggg gccatgccag cc
//
LOCUS       DAWAEA010000028         6744 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_1795_length_6744_cov_2.399462, whole genome shotgun sequence.
ACCESSION   DAWAEA010000028 DAWAEA010000000
VERSION     DAWAEA010000028.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 6744)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6744)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6744
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1795_length_6744_cov_2.399462"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6495..6504
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1302 a         2211 c         2002 g         1219 t
ORIGIN      
        1 cccccgcctc cgccgcgctc agggccgcca tcagccccgc cgcgccgccg ccgatcacga
       61 tcactttatt catgcagctt gtcgatatag acgctgtgcg cgttccatat tccctccaga
      121 cgcttgaaat gctcgctgag ctccgtgcgg cggctcatgc ccagagacac gatcagcgcg
      181 ttgatcagcg aaagcggcgc agccagactg tccacaaagc cggacatgtc cgtgtgagcg
      241 cacaggcaga tatccgattc ctcccgcagg gggctcatca cgccgtcggt catgccgatg
      301 acctgcgccc cgtgctgccg ggcgaagcgc atacattcca gcgtccgggc ggaataccgg
      361 ggaaagctga tgccgaacag cacgtcgccc ttctggatgc ggtcgatctc ctcgaacacg
      421 tcgccggtca gattttgcac cagcaccacc tcgtcgaaga tctggtgcag gtaatggtac
      481 agaaacatgg ccagcggcgc ggaagaccgc aggcccatga tatacagccg ccgggcggcc
      541 agaatgcatt ccaccgccat atcaaaatcc tgcgggcgca gctcgtccat ggtcttgcgc
      601 aagttttgca ggtcgttttt cagcaccgtg ctgaccacct cccggcggtc tgtctcctcc
      661 atcatgccta cccgctgctc ggcggtgagc cgctggcgaa ccatggagcg aagcttttcc
      721 tgcatttccg ggtagccctc gtagcccagc gccatggcga agcgaaccac cgtgctttcg
      781 ctgacgccgc actgcctgcc cagcatcacg gccgtcatgg acaccgcctt ttcatagtgg
      841 caggaaatgt actccgcgat gcgcttctgc cccttgctca ggggcttgcc gctcatattg
      901 agccgggtga tccattcctg tgggtcgtgc acttgatctt ctccttctgc gcggtcaggt
      961 cagcccagca gacccagccg gtcgaaatcc gcctgaggtt cgctgcccat gccgctcaag
     1021 agtgtcagag caggatgccc ctcattcaaa taccattcgt cgctgccctc ggcaggcggc
     1081 tccgattcgc agccctcctg caaccagaaa tacgtgccgg gcctgtcggc gtaacagcgg
     1141 tcgtagccgt cccggatgcc cgcgtcggac aggggcgcat acaccggtgc cttccactgg
     1201 gcgcggggca ggtcgggcac gttgatgttc agcacccgga agccgttttt gggcgcacgg
     1261 aagtatttct ccgccacttc cagcaccagc tctgccgcgt cctgcacatg ggcggggtcg
     1321 gccttgtagt caatggaggc cgcgatggac ggatgcccca gcagcgccgc ctcccgggcc
     1381 gcgcccacgg tgccgctgta atagacggcc aacccggcgt tgtagccgtt gttcacgccg
     1441 gagatcacgc cgtccaccgg ctccgtgacc agcccgccca agaacgccag ccggacgcag
     1501 tccgccggag tgccggtaat ggcgaagccc tgcgcgtttt cccggttcag ggggtaatcc
     1561 ttcacataca gcggatcgga aagggtgatc cgatggctgg cggcgctcat ctggccgctg
     1621 ggggcgcaca tgctgacccg gtggccccgc ttcagcgccg cttccatcag ggccataatg
     1681 cctgccgagc cgatcccgtc gtcattgacc aataaaaaat gcattgtttt cctccttacc
     1741 ggcgtacatc gccgctcaac gcgtcgatca ccattcgggc ggcgttcacc accgccgcca
     1801 ccgccagcgc ccaccagaaa ttctgaaact gaacgctgtt ttccaccagc gcggcagctg
     1861 tccacaccag ccacgcatcc accgccacgt acaaaagccc cagcgtgcag aagttgacca
     1921 ccttcagcag cagtctcaac aggggccgaa gcacggtgta gaccactgcc agcagcagcc
     1981 ccagcacgat ggcgttgctc atgtccaccg cccgcacgcc gtccatcagc tgggcgcaca
     2041 ggggcacggc cgccgccgtc accagaaaac gcaggatccc gcagaacatc tcatccctcc
     2101 cagtatacag gcagaaccag ccaattttca ttataacaca tttcccgctt tctgcatacc
     2161 cggcggaaaa gatcggccca aaaacgccca aaaatgaaaa aggactgccc tgaaaagagc
     2221 agcctccgct ttcactttcc cccatgctcc tttttccacg ccttgatggt ctccaggcgg
     2281 gtcttgaacc ggccttccag cccttcttcc gtgggatggt agtattcccg gcccaccaat
     2341 gaatccggca ggcactgcat ggcggtcagt ttgtccttat aatcatgggc gtacatatag
     2401 cccttgccgt agtccagctg ctgcatcagc cgggtgggcg cgttgcggat gttcagcggc
     2461 accggctcgg ccagctgagt caccgcgtcc ttcttggcct cgttgtaggc cacctccatg
     2521 gcattggact tgggagccag cgccagatag gtcacggcct gcgtcaggtg aacgttgcac
     2581 tcgggcaccc ctagaaaatg gcaggcctga taagccgcca ccgccagctc cagcgcccgg
     2641 ggatcggcca ggcccacgtc ctcgctggcg aagcgcacca cccgccgggc gatatagagc
     2701 gggtcttccc cggcttccag catccgggcc agccagtaga ccgccgcgtc cgggtcggaa
     2761 ttgcgcatgg acttgtgcag ggcggagatc agattgtaat gctcctcccc cttcttatca
     2821 tacaaaaggg attttttgga gatgcactgc tccagcacct cttcggtgac ggtggtggtg
     2881 tccccgtcca gatcgccgtt gagcaccacc atttccagcg tggacagggc ggaacgggcg
     2941 tccccgttgg caaactccgc cacggcccgc agaagctccg gggccatgac caccttctgc
     3001 ccgccgaaac cgcgagggtc cttcagcgcc cgttccagca gaccggtcag atcgtccgtg
     3061 gtcagggcgt gcagcacaaa caccttgcac cgggacaaaa gcgccccgtt gacctcgaag
     3121 gaggggtttt ccgtggtggc cccgatgagc acgatgctgc ctttttccac aaagggcaga
     3181 aaggcgtcct gctgggcctt gttgaagcgg tggatctcat ccacaaagac gatggtcttt
     3241 tcgccgtaca gggcgttgtg atcggcgctc tgcatcacct cgcggatctc cttgatgccg
     3301 ctggtgacgg cggaaaagtc gatgaacttt gccttcgtcc ggttggcgat aatgcgggcc
     3361 agcgtggtct tgcccacgcc cggagggccc cagaagatca tggaggaaat atggtcgccc
     3421 tcgatcagcc gccaaagcac ctttcccgga gccagcaagt gtcgctgccc cacaaattcc
     3481 tccaagctct ggggccgaag ccgggccgcc agcggctgca tgggctgcgg ggcggtaaaa
     3541 aagttctgct gttccatggg ttccctcctg cgaaaagcgc gaaaaatcag ttgcccttct
     3601 attatagttt cgccgggaaa gaagcgcaag tgcctccccg gcgtgacaag acggcaaact
     3661 atggtataat agcaaggaga atcctacgag ttgaccgccg ggggtgaacg gttccccctg
     3721 ctgaatggag gcctcatgag cgaaacgcgc aaaccctccg tccttctggt ggacggctac
     3781 agcctgatct tccgggcctt tcacgccctg cccctgatgg tgaccaagga tgggacctac
     3841 accaacgcgg tgcacggttt tttcagtatg ctgctcaaag cgctgggaga ttaccgcccc
     3901 gaggccctgt gcgtgatgct ggacgtccac gcgcccacct tccgccatac cctgtacccg
     3961 gattacaagg gcacccgcaa gcctatgccc gaggagttgc ggccccagct gcccctgatg
     4021 cgggaggtac tgaaagccat gcatatcgcc tcctttgaaa tggagggcta cgaagccgac
     4081 gacctgcttg gcacggcggc gcggctggcc aacgagcagg gctatgcggc ctatgtgctc
     4141 accggcgacc gggacagcct gcagctggca ggcggagaca cccgcattat cctgaccaag
     4201 acgggcattt cggaaagcct gctgctggac gcagagggcg tgaaggagca ttacggctac
     4261 cggcctgatc aggtgccgga catgaagggc ctgatgggcg acagcagcga caacattccc
     4321 ggcatcgcgg gcatcggcga aaagaccgcg ttaaaactga tctcccaata cggcagtctg
     4381 gacgaggtac tggcccatgc ggacgaggtc aagggcaagc tgggcgaaaa gctgcgggcg
     4441 ggccgggagt cggccctttt gagccgtgac ttgggcacca tccgccgcaa cgcgcccata
     4501 aagccggact ttgcccactg cacgtggcag gaactgaacg agggcatccc gcttttgcag
     4561 caatacgaac tgaaccagac cgccaaaagc gtggagcgtc ttttgagcgg cgaaaccgtt
     4621 cccgccgttc agccccgcac cactgccgca gggcagcggg cgggcggcgg caaaaaggag
     4681 accgggaaag cctctcccgc cacagccatg gcgcaggaga cggggctgcc ggggcttcag
     4741 gccgtttccg gggagaaggt cttcaccgac ccggacattc tgtgtcaggc cgtcgcggcg
     4801 ctggcggcgg aaaagccgga atttgccgtg ctccatctgg ggccggagga ggtcaccctg
     4861 ctgaccccct cgctggaatt atggcgcatg ccccttctgc gggatctgat gggcggtggg
     4921 atgtacgaag atcacattct gacggagctt cgtccggtgc tggaagccgt tcctctgatc
     4981 gtccacgggg cgaaggagct gttccatctg ctggagcgtt atcatctgcc ccaacccgcc
     5041 atccgtcagg acaccatgct gggagcctat ctgctgcaca cggctcagaa ggattataca
     5101 ctggccgcgt cactggaagc ggaatttgcc ggagaggccg tctccctgcc ggaaagcgcc
     5161 tctgcggcgg aagtctattc gatggctctg cgccagcggg agcggctcca tcagcgggac
     5221 atggaatccc tcctctggga catggagcag ccattgaccc gggttttatt tgagatggag
     5281 gacgaagggt ttctggtgga cttggacacg ctgaaccagc tcagcgacct tttcacccgg
     5341 gagatcgaag aaagccgtca ggcggtctac cggctgacgg aggtgccgga cttcaatctg
     5401 aacagcccca agcagctggg cgaagtactg ttcgacaagc tgggccttcc cgcccagaag
     5461 aagaacaaga acggggccta ttccaccgcc gccgaggtgc tggaggactt ggcgccctat
     5521 cacccctgca tcgagcccct gctgcgctac cgcaagctga gcaagctgca gtccacctat
     5581 gtggacggtc tgcggaagct gcggggccga gacggacggg ttcacaccac ctttgaccag
     5641 accgccacgg tcacgggccg catttccagc atggagccca acctgcagaa catccccatc
     5701 cgcacggcgg cggagatcca cggcgtttcc atggaagacg tcacgccgga aatgcgccgg
     5761 gcggcaaagg ccaccaactt cggcatcgtc tacggcatca gcggcttcgg ccttgcccgg
     5821 aacgcgggca tttcccgccc ggaggccaac cgcttcatcg agcgctattt tgagcgttat
     5881 ccgggggtgc gggccttcat ggacgaatgc gtccgtctgg gctacgaacg gggctatgca
     5941 gagacgatgt tccaccgccg ccgggagctt ttcgagctca gctccaccaa ccgcaacgtg
     6001 cgcaatttcg gtgagcgcgc cgccatgaac acccccgtgc agggcacggc ggcggacatc
     6061 atcaagctgg ccatggtaca ggtggagcgc cgtctggaag ccgaaggcct caaggcccgt
     6121 ctgatcctac aggtacacga cgagctggtc gtcgagtgtc ccatcgagga agccccccgg
     6181 gcctccgccc tccttcagga ggtcatggag aacattgtca ctctccgcgt ccccctcatc
     6241 gcccaggtct cccaaggcaa agactgggaa tccgcccact aaggggtagt accccttaac
     6301 cccggactgc gcgcctatgg cgcgcagggt gctcggggga catgctggtt agccgtaaag
     6361 ctaccgccgc tcggcgacag gcctcgcggc tcgcgggttt ggtgtggctc cctgttagtt
     6421 gaatagaaaa gaccgccatt cggcaacggg cctcatggct cgcagcaggg gcagcgccgc
     6481 ttgaccccac accgnnnnnn nnnntggtgt gggttcccat ttgttgatgt gtacagaccg
     6541 ccgtcgggcg acaggccctc cggctcgcgt cacaggccaa catgatttgg atccccctta
     6601 gccgtttgcc ttgcgagatc aaccgcccca cgccgaatca tcccaactcg cgctatttga
     6661 ccttcgcccc cacccaagcg gcggcgcaga cgagcggcga taggcagcgg agggaaaaag
     6721 gcccgcccat tttgacggcg gaaa
//
LOCUS       DAWAEA010000029         6629 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_1834_length_6629_cov_2.761485, whole genome shotgun sequence.
ACCESSION   DAWAEA010000029 DAWAEA010000000
VERSION     DAWAEA010000029.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 6629)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6629)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6629
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1834_length_6629_cov_2.761485"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1277 a         2105 c         2061 g         1186 t
ORIGIN      
        1 gaacttgaac gcgccgtggc tggtgccgat ggcgatggcc aggctgtcgc agccggtgcg
       61 ggtggcgaac tcttccactt cgtcgggatg ggtgtagctg gaatcctcgg cggaaacctt
      121 cacatcgtcc tccacgcccg ccagacggcc cagttcggcc tcaacggtga cgtagttctt
      181 ctggctgtga gcatattcca cgaccttctt ggtcagagcg atgttctctt cgaagggcag
      241 gtggctgccg tcgatcataa cgctggtgaa gccgccgtcg atgcagctct tgcaggtctc
      301 gaagtcgggg ccgtgatcca gatgcaccac gatgggcaga tcggtatcct cgatggcggc
      361 ctcgatcatc ttcatcaggt acacgtgctt ggcataggcg cgggcgccct tgctcacctg
      421 caggatcagg ggagcgtttt ccatcttcgc tccctcggtg atgccctgga tgatctccat
      481 attgttgacg ttaaaagcgc cgatcgcgta tccgccttcg taggcctttt tgaacatttc
      541 cttcgtattg accattggca tgggaaaaat tcctccttta atcagggtga tatctgcatt
      601 atacatgatt ttttcccggt tgaacagccc aaactttcct ttagagggca gaaacctttc
      661 cgagcggatc ttttcaggct tcctccgccg ggtcgcgccg ccgggcgggg cgcttttcgt
      721 cctcgggata gatatagctc ccggcggcag ggcgcgacgg cgcctttggc cacccggtgt
      781 tttttcatca gccgtaccca cagacggccc tccgatcgtt ttgccggcac agcaaaacct
      841 ccttttgctt tttctcttct aaacggccct gccccaacgg gattcccggc acggcctgtg
      901 ctcaatgcag cgcctctccc agcgcggcga cctgctcgtt ggacggggag tgattcttct
      961 gcttatagtc cattccccaa tcaaacatcg cgcccagaac cggcttcagg ctctcgcccg
     1021 tttccgtcag ggtgtattcc acccggggcg gcacctcggg ataggctttg cgcgtcaaaa
     1081 ggccgctggc ttccatatcc ttcagatttg ccgtcagcac tttttgggat acgccggcca
     1141 gcgacttttt cagttctccg aagcgcttgg tcccagtgaa cagatcccgg aggatcagca
     1201 ctttccagcg atctgaaatc agggaaagcg tcgtttccac cgggcacatc ggaagttccc
     1261 tgttttctgc catgaaagca cccctccatg cattgcgggt ttgtgaacca gtcagaatga
     1321 ttataacgac ccgtatttga tccgtcaacc gattttcaga cgggcgcagg gcaatggaaa
     1381 aggagattcc gtaccctcag ccattttttg catcctgcca gactgccgtc ttcctccttc
     1441 ttcccttttc ccccttctgg gacaagcaca ttgacggata gtgttttttc gctataataa
     1501 ggtagaatag agcgaattat cggcatatgc cgatgcttat gtatgaagga gagaagtccg
     1561 atgcctgccg cttccgctta ccgccgcgcc cctttggtca accccgccta tgtgcccttg
     1621 tccgacgccg ccctgcaccc cgaagggacg atgaaagccc ggatccagca ggtgctgaac
     1681 atggtcaaca cccgccatat gtccacgctg gactccgtgc tggccaccaa gctgggcggc
     1741 tatgcccagc tgccccccat gcccgacgtg gaggccgccg cggcgctttt gcccgccgat
     1801 ccgtcccgct ttgtggagat cgtatggagc gcctccatgc tggcggccat gagccgggat
     1861 aaagagggaa tgctgcgggt gctgggagcc atgcgggcct ttgcccaggc gctgccccag
     1921 ctgagcgagg aagccctgtt cgcctgcggc gcggacgcgc tgcggctgac ggtcgatctg
     1981 taccgccgca cgggccagcc cttccttttg aacctgctgg agaccatgcg gggccagctg
     2041 ccggacgtgt ccggcctgat gcacatgttc cccttccagc gggagtaccg gcgggaatcc
     2101 cagggcaata ccccggagga aaaagccttc tacgaccgta tggatcgctt cgccatgggc
     2161 aagggcgcgg cggacgcgct ggccatgacc gtctttctgg gccagtacag cggctccggc
     2221 cgggacgcgg ctgcgccgct gacgggtctg aacgccctga accgctatca cggaatgccc
     2281 tgcggcgcat ttgccgccga cccctatctg gcgggccggg accccgcccg ggcggtggag
     2341 ctggacgcgc tgtgcgcgca gatcgaggcc tacgcggacg cgctgtgctt caccggtgaa
     2401 aacgcctttg ccgagcggct ggagctgatg tgggaaaacg ccctgcccga ccttctgacc
     2461 gaagaaggcg ttcgcacgtt ggaacccacc aaccgcctgt cggacgacga gagctgtcag
     2521 cttcacgccg tgcccgaaaa ggaggatgta agcgccctgc tgcgggcgct gtacgccctg
     2581 cggcgtagcg tctggctgag ccgggacgac agcaccctga cctatatgct gcccgtggag
     2641 agcggctgca ttacccggct gggcggcgtg cccgtgcgca tgaccgccgc tgtgaaggga
     2701 ttgagccgca aggagatcac cctgcaggcc gaactgaagc agcccgtcca gttcacgctg
     2761 gaactgcggg tgccccgctg ggccgacagc gccacggtca gcgtaaacgg cggcaagcct
     2821 gtgcaggctc agcccggaga aaaattcccc ctgacccggc tgttccagag cggggatacc
     2881 atcgtgctga cgcttgcctc gtctccccgg ctggaaaccg gctaccgggg cagcctgtcc
     2941 gttttctgtg gcagcacgct gatggcgctg cccctgcccg gcgcagagat ggaatggcgc
     3001 tatgcgctgg tgtccggcga aagcgtcacc gtcagcgagg aggacggcga gctgaacgtg
     3061 ctggtcacgg cctgtgaagc gcccctttgg aaagaaaaga agggctttat cctgcccccg
     3121 ccccagaact gccagatggg ccccgcttac cgtctgaccc tgatccccta tgcgggctgc
     3181 ggcggacgca ttgccgcgtt cccctgtgtg cgggcataaa agatgacgga aaagaatggg
     3241 atgaatttgg agcggctgcc cgttttatca ttagcgccca tggcgggcat caccgactgg
     3301 cccctgcggg tgctgtgcta ccgaatgggc gcggaatacg cctgttcgga gatggtctcc
     3361 gccatcggct acctgtgcgc caagcccggg aaccgggtat atacccagct tttgcaggta
     3421 cacccggagg agaccaatac cgcatgccag ctgttcggca aggacccggt cgtcatgggc
     3481 gaggccgccc agaggatcac cgagctgggc cggttccgct cgctggatat caacatgggc
     3541 tgccccgccc ggaaggtggt ctccatcggg gaaggcagcg cccttttgct caaccccgat
     3601 caggcctacc gcgtcatgga ggccgtggtg aaccacaccc atctgccggt cacggtgaag
     3661 acccggctgg gctttgacaa aagcaccatg aacgccgtgg agctgggcaa ggccgcccaa
     3721 tcgctggggc ttcgctggat gtgcgtccac ggccgtaccc gggagcagca gtactccgga
     3781 caggccgact acggcgccat cgcccgggtg cgggagcagc tttccatccc tgtgatcgcc
     3841 aacggcgacg tattcgcccc cgaagacgcg ccgcgcatcc tgcgggagac cggctgctcc
     3901 ggcctgatga tcggccgggg agccatgggg aatccgtggc tgttccgggg cgcgcgggag
     3961 gcgctggacg ggcggcccgt cacgccggtc acggcccggg agcgcatcga cgtggcgctg
     4021 ctccatctgg aatggatggt gggctacaaa ggcccccggc agggcgtgct ggaaatgcgc
     4081 aagcatctgg gccactacat cggcggaatg cgcggagcta cggccctgcg ccgggaactg
     4141 aacatggcca aaaccgagga ggagctgcgc ggactgctcc tccgcctttg ggaagaagtc
     4201 cgcggtcagg aggatcaaga atgaaaaacg caatcaaaag agcggcgaag gcgctggcgc
     4261 tgatcctgtg cctctgcctg acggcgggcg ctgccccggc caaagcggac gcgctggaca
     4321 tggtcaatct gctgtctccg tggatcgagg gcgacgaagc gcagctctcc gtcagctacg
     4381 agatcaagaa cctgctgccc tataacgaca gcaccctgac cctgctcaac aacgtgctca
     4441 agcatatcac cgtggagggc cgcgtcagcg ggaatgatac ctccatgggc atttcggtgg
     4501 acggggacag tctgttcacg ctggaggaga cgcaggaaaa cggccgctcc gtgcttcgga
     4561 cggagctatt gcccaaccgg gagctgacct ccgcccaaag cgcgctggac gcgctcttcc
     4621 cggaggacga gaccgctgaa ccggcagaag aaggcacgga cgcgctgccc ggcggttcct
     4681 ttgacctgaa cgcggccctg accgaggcgg aagcctgcta ccgccagctg accgacggga
     4741 tccagcccta cgccgaggaa aagaaagcca actacacgat caaaaacgtg ggcaagggca
     4801 aatgggtgcg gctggccaag ctgaccgccg aacaggccga gaccctcaag ccccagatcg
     4861 cggcggtgct gtcctgcggc atggacgaag ccttccgcca gcaaatggaa agcctgaccc
     4921 ttaaaaaggg ctttaccgta gccctatacc gcaccgccga ggatggggac gatcttgccg
     4981 tctacatcaa gggcaacgcg gccatcgagg acgtcaacta taccatcgcc taccagtggg
     5041 ccttcgcccg gggcgaaaac ggcaacgata cggacaccta caagctggaa tttaaccggc
     5101 ccaaagggaa gatcgacaag cggacggcgg aggccagccg cagccagtcc gtcagtgagg
     5161 acgggcggaa gctgaccgtc cagagctcgc tggaattgaa ggaaggcaag ctgaccctga
     5221 ccaccacccg gaaatacgac cttaccggcc gggacgcgga cggcggcaca gcggtgtccg
     5281 gcagcatcgt ggacaccgtc aagcagcagg attccaagga aaagaccagc tccactacct
     5341 ccaccgagct ggtgcccgag ctgaaatggg cgggcggtgt gctcagcggc acaatcaacg
     5401 tagcccggct gaacggcaaa actcccgcgg gcgaatatgt gcttcacttc ggcgatacgt
     5461 ccttcgaccc ggtgcaggca ctggccgctg ccagcagcag cgacgactcc gcccccaccg
     5521 tgcagatcac catcgacggc tccagcgtgc agcagaacac ggacacactg caggccatgc
     5581 agtccggctc ctatcaggta ggcgaaacac ccgtgggcgt gacggaatac accgccccct
     5641 ccaccctgac gaccgtcgat ctggacaccg ccgacgcggc cgcgctggcg gcgctgcagg
     5701 gcgagctgtt ccaacgggcg gcgggcaagc tgctccccgc gctggcggcg ctgccggacg
     5761 gcgactccgc gctgctttcg gattccatgt ccgacgagga ttatcaggcg tttttgaacc
     5821 tgctgggact gtaaagctca agccatcaaa ggaggccgac ccatgggaaa acggaacgga
     5881 ctactccgct tctggggcgc gctggcgctg tgcctgtgcc tgtgcactgg cgcgctggcc
     5941 gaaagcctgc agggctacac ggtggcggaa aagctgatca agcagctgga cgcgggcagc
     6001 ggcttttccg gcacgctgga agtgcagacc gacggggccg acgcgcccct ttccttccag
     6061 tgggactaca tccacacccg gaaaaacagt gagaccgtgg agcagcagcg gctggatctt
     6121 cggctgatgg acggcgagac cgcccgcaat accttccgct ttcaatggga agacccggct
     6181 ctgcgttttc agaccgacct ttggtccggc cagtggtacg agctcaacgg aacccgtacc
     6241 gagcaaagcg agggcagcgc gggtgcagcg ctggacactc tgctggccga cacgctcaag
     6301 gcaaacggca ttcccgtatc ctttctgcgg atgctgccgt tgctttcctc gatgaacacg
     6361 ccggacgaat ggctggcccc cgcactggag acctgcctga cccggatgga catctggatg
     6421 gagggctacc ggggacgcac agatatcggc aagctggagg acggcacagg cacgctcagc
     6481 gcccagtacc gcatcgcgcc ctccaacgtt aaggcacagc tgaagcagct gattctggac
     6541 gtgctggggg acgaagacct gatccagcgg ctgaaaaatc actttggcga ggaacagacg
     6601 gccttgtatc tgaacccgga atggcagcc
//
LOCUS       DAWAEA010000030         6527 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_1867_length_6527_cov_2.357849, whole genome shotgun sequence.
ACCESSION   DAWAEA010000030 DAWAEA010000000
VERSION     DAWAEA010000030.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6527)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1867_length_6527_cov_2.357849"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2386..2395
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4409..4418
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1216 a         1900 c         2174 g         1217 t
ORIGIN      
        1 gagccgttgg gccgggccgt cgtcgcgctg ggcctttgcc tgtcgctggc gggcggtctg
       61 ggctgccgct atccgggaga tacgctctac ggcgtgcaga atatgagggg caacagcgct
      121 tggatgagtc ttgtaagcgg cgaagcggcg cagtacgacc gggaaatgac ggaacgggag
      181 cggcttctga atgacgatac ccagcccgtg gtaacgctgg aaccacttac cgccgtgccg
      241 cgcatcttta tggaggatga gctggaaccc ggcgcggtct acgacgtgcg gcccagcctg
      301 tgccgctact acggcaagga agccattctg ctggcgggag aggaggaacg gccgtgaaag
      361 cgttttgtcc ttcttcaacc acgccagcta cgacgatctg ggctattcca tccgcaccca
      421 cgagcgctgg cgggaaacgg gcagcttctg gcagacagtg gaagcagcgg cggaaaatac
      481 ggcctccatt cgttacacat ggcaggggac gttcttctcc tgctttctgg cggcgctgca
      541 gcccgccttg ttcggggaaa gctggtactg gatcaccacg tgggtgctga tgggaacgtt
      601 cctgctggcg ctgggcgcgc tgacccggca gctggttcgc cgggtgatgg gcgcgggcac
      661 ggcggaatgg ctgatcctgt tttccctttc cgcgctggta atgatgcagc ttacccccag
      721 catcagcgaa gcctatttct ggtggaacgg cggcatggtc tattccgtgg tttggtgctg
      781 tctggccctt cggacggctt tatggctccg cttggaggac agccggaagg ggcggcttgc
      841 gtgctttttg agcatggcgc ttttgagcgt ggcagtgggc ggcggcaatt tcgtcacgcc
      901 gctgttcggc tgcgcgctgg acgcgttgct gctgttcttc tccttccggc agaaggaaaa
      961 gagccgcttg ggcaagctga cctgcttcgt tctcatgctg gcggcctttg tctacagcat
     1021 gaccgccccg ggcaacgccg tgcggggaca gaccctttcc gggggcatga gcgccgtcaa
     1081 ggccattctg gaatccttct atttcggcat tgcactgatg agccagtatt tttctccggc
     1141 actggcggcg ctgtggctgg caacggtgct gcttctcagg gagcggctga tgcagagccg
     1201 gttttccttc cgttatccgc tgctggcggt gggcgttttc gtctgcctgt actgctgcca
     1261 gctcacgccg accctgtaca ccggcaacta tctgggagac ggccgggcgc aggtattgca
     1321 gtatgacaac tatgtgctgc tgtccacggc gctggtcgtg tatctggccg gttgggtgat
     1381 gaaacggtac ggaaccggac aggtcagccg cccgccgacc cgggccgggg ccgctgctgc
     1441 ggcggcgctg cttctgctgg gcatgatgag cttccagcgg acggacgggc agcttccggg
     1501 cgtacagaac accctgaccg tcagcaccct tcggtcgctg gtcagcgggc aggccgccgc
     1561 ctttgacgag gccatgaccc gccgggacga tgaaatgaac gatgcatccg tccgagcggc
     1621 acaactggat ccggtggagg acatcccgga ggcgttcatg ggcgaggtgc tggatacgga
     1681 catgaagaac tacgtgctga gcctgtatgc cgagtattat cagaaggaca gcgttgcgct
     1741 gaaaacagag gcgcaggacg aaccataaga aagggggaac cagcttgctt ctggaaggaa
     1801 agaatatcgt gctgaccggc gcgaaccggg gtatcggcaa cgccatgctg caccgcttcg
     1861 ccgaggaggg gggcaatctc tgggcctttg cccgcaggcc ctcggagact tttgaacagg
     1921 actgccggga aacggcggag cgcttcggcg tgtgggtgga gcccgtttac tgcgagctga
     1981 ccgacacgga ggccctgaag gcggccttca agcagatcat ggtggagaaa aagcccctgc
     2041 acgggctggt gaacaacgca ggcgtcatgg gcgaggataa gatgttccag atgacctccg
     2101 ccgaggaaat gcgccgcacc tttgaggtaa atttcttcgc catggtggag gtcagccgtc
     2161 tggccaccag gcttatggcc cggaacaaag ccggagccgt ggtcaacgtg gccagcatcg
     2221 cagggatcga cggcgacagc cggttggact attccgcctc caaggccgcg gtgatcgccg
     2281 ccagcaaaaa gatggcccgg gagctggtca gcgtgggcgt ccgggtgaat gtgctggctc
     2341 ccggctttac ggataccgag ctggtggcgg ggctgagcga aaaagnnnnn nnnnntccaa
     2401 aattctgatg caccgcaagg cccgcccgga ggagatcgcc aacgtggcgg cgttcctgct
     2461 cagcgacctg tccagctacg tgaccggtca ggtctggcgg gcggacggcg ggatcctttg
     2521 aatttctggt tttctaaaga aatcatagag tacgacagga gtgcatgaaa atgacgaaca
     2581 tggaaaaata cgtgaaggct ttctccgagg cgctggaagt gcccgaatcc gacgtacccg
     2641 ctctggtgta cggcaagagc gaggcgtggg acagcgtagg ccacatgacg ctggtggccg
     2701 cgctggagga tgcgttcgac atcatgatcg acatggatga tattattgac ctgagctctt
     2761 ttgaaaaggg aaaggagatc ctgaagaagt atgatgtgga actttgagcg cttcggggac
     2821 aggacggcgc tgctggatga acacggcgcg agcctgacct accgccaact gaagcgggag
     2881 gccgacgcgc tgtgcgccgc cgccggcgga cgctgcctga ccttcaacct gtgccgcaac
     2941 acgctgggca gccttttggg ctacgcgggc ttcgtggagg gcggcgtggt gcccgtgctg
     3001 ttgaacgagg aaatggatcg ggagctgctg aagggcctgt atgacaacta tcatccggcg
     3061 tttttgtggg ccccggagga tttcgcatgg gacggctgcg agccggtgta ccaaagcctg
     3121 ggctatcacc tgctgaaaac gccctttggc cgggaagcgg agctgtatcc cgagctggga
     3181 ctgcttttga ccaccagcgg ctctaccggc agccccaagt ttgtgcggca gagctaccga
     3241 aatatccggg ccaacacgga cagcatcgtg gaatacctga agctggatga aaccgagcgg
     3301 cccatcacca ccctgcccat gaactacacc tacggcgtta gcatcctcaa cacccatctg
     3361 gacgtaggcg cgaccatcct tgtgacggag cacggcatcg cccagcggga attctgggat
     3421 ttcttccgcc gggaaaacgc caccagcttc ggcggcgtgc cctacaccta tgaaatgctg
     3481 gaccggatgc gcttcttccg catggatctg cccagcctgc cgtgggaaaa gagtctggaa
     3541 aaggtgggcg gcattggaat cgccattccc ggcggacagt tccggctgat cgccgccgac
     3601 ggcagcgaaa tcacccagcc cggcgtgacc ggcgagctga aatactacgg cgataacgtg
     3661 acgctgggct atgccgtctg tcaggctgac ctgcgcaagg gcgacgagcg gggcggcgtg
     3721 ctggaaaccg gcgatatggc gcaggtggac gcggacggct actataccat cgtgggccgg
     3781 aaaaagcgct ttttgaagct gtacggcaac cgggtcaatc tggacgagct ggagcagctg
     3841 ctcaaggcgg cgttccccgg cgtggacgtg gcctgcggcg gcgtggacga tcatctgatg
     3901 ttcttctcca ccgacgaaaa caggctggcc gagatgcgcg ccttcctgtc cgaaaagacg
     3961 aagcagaacg ccgccgcctt ccggggcatg gcgctgccgt ccattcccaa aaacgacgcg
     4021 ggcaagaccc tgtaccggga gctggagaaa tactatgatt gatctgcagg cccttcccga
     4081 gctggccccc tacggcatgg cccgggagga aaagcgggcg tttctggccc gggaactggg
     4141 tgagctgacc cgttttcacg gcgaacactg cccggaatac gggcggctgc tggcggcact
     4201 gggcggacag ccggggcaga cggacgattt tgaaagcctg ccctttctgc ctgtgcgcct
     4261 tttcaaggag ttttccctga aaagcgtgcc ggacgaagcg ctggcccgca ctatgacctc
     4321 ttcgggcacc acgggccagc aggtcagccg catctatctg gatcgggaga ccagagcttt
     4381 gcagtccaag gtgctgtccc gcatcatgnn nnnnnnnncg gggatcctgg ggttcagcat
     4441 gttcggccgg gacaagatct acgcgctgga cgagaacatg cagctggata ccgaaggcct
     4501 gaaggccttt ctggaaaagc acgaagggga ggatattttc ctcttcggct tcaccttcat
     4561 gatctggctg tattttgtga aggaacttcg ccgcacgggc tatcgtcccg atctgagccg
     4621 gggcgtgctg gttcacggcg gcggctggaa aaagctggcc gaccagcata tctccaccgg
     4681 ggcgttcaag caggccctgc aggagacctg cggtctgacc cgcgtccaca attactacgg
     4741 catggtggag cagaccggct ccatctacat ggaatgcgaa tgcggccatc ttcacgcgcc
     4801 ggtgtggtcg gatgtgacca tccgccgccc ggaggatttt tccgtggccg caaccgggga
     4861 aaaggggctg gtgcaggtgg ccagcgtgct gccccgcagc tatcccggcc acatcctgct
     4921 gaccgaggac gagggacgca ttctgggcga ggacgactgc ccctgcggcc gcaagggcag
     4981 gtattttgaa attctggggc gcattcagcg cgccgaggtt cgggggtgca gcgataccta
     5041 tgagcaacgc cgttgaacgt ctttgcggac gggagccgga aagcgggctt cggcccctgc
     5101 ggccctttgc gccggaggcg ctggactttc tgtcggccct gtccgcagcc cttttgaagg
     5161 atcgggaggc gaaggcttac ccggacgtga tcacctttgc ctatttctgc cgcaaggccc
     5221 atctggcccg gctgcaggag gaatacgccg ggcagctgga cgaccggctg ggccggggcg
     5281 tggtgttcca catcgcgccc agtaacgtgc ccatcaactt cgcctactcg ctggcggcgg
     5341 cgctgctcag cggcaacggc agcgtcgtca aggcctccag ccgggatttt gtccagaccc
     5401 gcatcgtctg ccgggccatg gaaaagctgc tgacggagga cttcgcggcg ctggccccct
     5461 acgtctgcgt ggtgacctat ccccgggagg cgcaggaagt gacggaagcg ttcagcgccc
     5521 gctgccacgc ccgggttatc tggggcggcg acgaaacagt gcgccgggta cgggaagcgg
     5581 ctttgccgcc ccgggccgtg gaggtggcct tcgccgaccg ctacagcctt ttgtgcatcc
     5641 gccccgaagc ggtgacggcc ctttcggcgg acgagctgga acggctggca aaggatttct
     5701 ataacgacac ctatctcacc gaccagaacg cctgcacctc gccccggctg atctattggg
     5761 cgggagaggg agacagccgg gaagcgtcgg agcgcttttt ccgtgcgatg ggcgcttatg
     5821 cccggcagcg ttacgatctg cccgccgtga cggcggtgga caagctgacc gccgcctgcc
     5881 gtgccgccat gaagctggaa ggcgcggaga tcgtccatgg ccccgacaac tgggtcacac
     5941 gggtgcgggt aaaggcgctt tgccccgagc tgtacgagct tcgaagcccg ggcgggttct
     6001 ttctggaata cgccgcgccc acgctggacg cgatgcggga tttcgttacg ccccgggtgc
     6061 agacgctgag ctatctgggc ctttcaccgg aggagctgag ccgcttcgtg ctggaaaacg
     6121 gcctgaccgg catagaccgc atcgcgcctg tgggacatac catggatttt gccctgacat
     6181 gggacggcta cgacctgatc cgtaccctgt cccggaagat cagcgctctg taagcaacgc
     6241 aagaaacgag ggatcctacc gatgaacaag cccatgcttt ccttcgtcat tccctgctac
     6301 cgctccgagc ataccatcgg cgcggtggtg aaggagctga cggacactct ttccacccgg
     6361 ccggaatatg aatacgagat cgtgctggtc aacgacggca gcccggacaa caccgcgggc
     6421 gcgctggaag cgctggcccg gcagacgccc cgggtggtgt ttgtggatct gagccgcaat
     6481 ttcggccagc acagcgcgct gatggcgggc tttcatcagg tgaaggg
//
LOCUS       DAWAEA010000031         6464 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_1893_length_6464_cov_2.656577, whole genome shotgun sequence.
ACCESSION   DAWAEA010000031 DAWAEA010000000
VERSION     DAWAEA010000031.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 6464)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6464)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6464
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1893_length_6464_cov_2.656577"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3231..3240
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1278 a         2090 c         1913 g         1173 t
ORIGIN      
        1 acgccagaag ggaaaaggcg caagcagatg gcgcaggtga atctggaaat agtattatac
       61 ctccaccacc cccgtggcaa cccgctccac aaacaccttg tcgtgttcca ccagcagcat
      121 cgtcggctga tactgccgaa tcagtgtctc caactgcacc cggctgaaca cgtcgatgta
      181 gttcagcggc tcatcccaaa tgtatacgtg cgcaggtgtt gcaaggctca aagccagcag
      241 caccttcttt ttctgcccct gactgtagtc ccgcatatcc agcgcaaact gctcccggga
      301 aaaatccatg ttgcgcagaa tggctttcac cagcgtttcg tccgcgcccc gggcggcgat
      361 ggctccttcc agcgggccgg acaggccgct cgtgtcctgc ggcacatagg agaccaccag
      421 cccggaggcc agacggacgc tgccgtgcag cgcttcgccc tcgcccacca gcgtcttgag
      481 gatgctgctc ttgccgcagc cgttggggcc ggtcagcgcc agccgttcgc cctgacggag
      541 gatgaactgc acgccccggc agacttcccg tccgtcgtag cagaccgcgc cgttttccac
      601 ggtgatcagc tcctgcttgg gatggtgcag tgtgcgcagc ttcagatcgc ccacccgttc
      661 tacattgtgc aataggccgc tcttttcctc ggcggcacgt tcccggcgct gcaccgccgc
      721 cttggcccgc ttcatcatgg cggcgctgcg cgcgcccgcg tagccccggt ccaccgctgc
      781 ctggacgctt tccggcacgt gaaatttgtc cttctccgcc ttctgggcga actgcgaagc
      841 ctgacgggcg ctctgattca gccggccgat ctcttttttc agcccctcgt tccgcttgag
      901 ctcgtaggcg ttctgccggt caaactgctc ctcccaggtg tcataatcgc cctgaatgac
      961 ctgaaagccg gttttgttga tgctgaggat gtgatccacg cagtcgttca aaaaggcgcg
     1021 gtcatggctc accagcagaa agccgtcctt tcggcgcagg tattccgcca cggcctgacg
     1081 acccagcaga tccagatgat tggtaggctc gtcgatcagg ggataagcgt cctcccgggc
     1141 gaacagagcg cagagcagtg ccttggtctg ctcaccccgg ctcagggtgg agaaggaacg
     1201 gtacagcgcg tcctccgtca cttccagcag ccgcatttcc ttcatcagct tccactccgg
     1261 ttcccctccc gcggcctcct gcatcacgtt cagggtcatg tcctccggat cggacacgga
     1321 atagggaaaa tagacaggct gaaggggcag gctgatctgc ccgccgtggg gcagctcgcc
     1381 catcatcagc cgcaaaaggg tggttttgcc ccggccgttt cgcccggtca gccccagccg
     1441 ccagccggtg tccagctccg cgctccagcc ggggaatacc ggctcactgc ttccatcata
     1501 ggtaaaagtc aaattctgaa tcgaacagcg catgaaaaaa gcctccttcc tgatagggca
     1561 ggggaggcat ggcggcgcgt ctgaaaaacg cgcaaaaaaa gcggagtcct cgggactgcg
     1621 ctttttcact gccacggcaa aacaagccct gcggaaacat tcatccgccg gggtctgttt
     1681 ttcctccccc attcaaatgg gttcatggca gtgttatttg cgcatcccga ccctccgttt
     1741 cgtttatcaa attcggggaa cgtcatcgtc tgcgttccaa ttcattgtag cagactcgct
     1801 gacagagtgt caagaacgat ctttttcttc cccgatcagg ccttttttgc ggtacaggta
     1861 cacctcgtcg taccagaagc cctccggagt ctccgccgcg gcgatcaggt cgtaggtctt
     1921 caaaatgtcc tccggctgac ggccccgggt aacgacgtag tcgcacacgc cgtcgtgaat
     1981 gtagcgcacc tgttcgtcca gcatctcctg caggggaacg ttggtctggt ggtaatactt
     2041 tacgttaggc gtgatctggg cggcggtgta gaagcccgcg tccataaagc cgtaattgag
     2101 cagcgtggcc cccggcgttt cagcgatctt ggcggcaaac tggtactgca tggtctcctc
     2161 ccggggcagg ccgaaggact ccttcacatt ggggctgaaa accgggcaca gcagcacgca
     2221 cagcccgcac aaagccgccg cgatacaggg ataagcccgc gtggacagcc cggccagccg
     2281 cttttccagc agaacgccca gccagccgaa aaagcagggc aggaacgccg ccagcgccag
     2341 cggataatag gggtaggtct tgccgccgat gaacacgccc agcgctccca gcaggaaggg
     2401 cagaagccag ccaatccgct gccagccggt cttgccccgg ccaaagcagg acgcaatcac
     2461 cccggcgcag actggcaggg cacacagggg gcttcggctc agccattccc agccgctttt
     2521 cagaatctgc ttcgcccgcc agagcagccc gccctcgccg ctggagtaaa ggaatagatt
     2581 atcatacaaa taagttttca gccagtcggc caacgcgccg ttcaggccga aatagatcag
     2641 ccacggcagg gcggacagca gaaagcccag aagcagcccg cccagagagc gaaagaatcc
     2701 cttcgccccg ttttgccggg cagccagaag gcagcccagc agcagagcca ttccctgcgt
     2761 cccgcagagg gtgaatttca tccagaacac gcagccgaag agaaaaccgt cccccagcag
     2821 ccgcccgagg gaaacgggct tttcgctttt cagcgactcc agccagtgca gctgcaccag
     2881 caggatcatg ggcagggcca gttcctccgc actgtcccct gcctgaaagc agtaggacgt
     2941 gtacacgcac agcgccgtca gcgggatgga gagcagcgcc gcccgtcggc ccgccatggc
     3001 cttgaccgcc cggtagcctt ccagcaaaaa ggccgccgca aacagaattt ccagcaccca
     3061 tacgccggtg aagtccgccg ggcagaccag cgcgcacagg gcgtggagag catagatcag
     3121 cgggcctttg tggtcgtaca ggtcgcggta catcacccgg cccgccagca tccccttgcc
     3181 cgctgaaaaa taaatattcg cgtctgacca gtcgttgatg ggataggccg nnnnnnnnnn
     3241 gaaaaacagc gccgccgccg cgacgaccag cgccagcagc cccattcgcg cccattcccg
     3301 ggcagaaagc ttcgtcaatc caagcgcctc cttataaaaa gcatgaaacg cctcttcaat
     3361 gtagcacaaa cccgctccgt gggcaagcat ttttccccct tccgccgcat accctgtggc
     3421 gaacgagggg aggaatggcc atgcgccgtg gcgcggtgct cagcgcgctg ttattgaccg
     3481 cgctgcttct ctatttatgg cccggactgc tgaaaggact gtggacggac acggacaacc
     3541 gaatggccgg cgagctgcgt cccgcccagc gccgcaccct gaccctgtgg tggttccccg
     3601 gggaaatggg cgatcagcgc tgcatcagcc gcctgtgtac ccgtctggaa aaggaaaccc
     3661 ccggactgcg cgtctttctg cgccgggcgg acgcggcgga gctgacggct cccgacgcgc
     3721 ttctgccgga cgcggtgctg ttcggcacgg gggacgtggt caatcccgag cagctgtttc
     3781 ttccgctgac gctttcggag gacgacggga atctttcctc cgggaaaagc ggcggcgaga
     3841 cttatgccct gcccctctgg tacgaggaaa acgtgctgag cgttccgaat ggctggctgg
     3901 aagaaaacgg aacgcccacc gttaccgtcc agccccattc cttattggga cagaccgccg
     3961 ccaccccaaa accggaaacg gacgagacac cgtctatccc gtggcggagg ctattggcag
     4021 aaaatgcgct gtgggtcagc cccggcacgg cagggccgca gctggcgttc accgcgccgt
     4081 cttctttgcg ggaggagctg gtctcggcgg cgcttcattc ggcggtaaaa ccggtcaagg
     4141 gtacggcacg ggtctggacg cgtcgggagc atctggccgc cgctcaaaag gaagccctga
     4201 cagccttttc actgcggcct gctgtcagcg accgggtacg atatgcggcc ctttgccggg
     4261 acacggcgga gacccgcgcc ctgcttgcct ttctccgctc ggaggactgc cgggaggaag
     4321 cggaagccgc cgggctcctg cccgccgaac aggcggattc cctgcccaac gccttcgccc
     4381 acacccggga ggagctgaac gccctctgcg cggacggcct gcgacggggc cttgacccgg
     4441 tggaaatcct gcttcggctg cgctgaccgg gccgggcttt ccatgcatag ttcccccgaa
     4501 aattaccata ctaagggcat gaatgaaact tcggaggcga taccatgact cagatcacga
     4561 cgcatcatcc ccgcagcggc tggaaggaag acgaagcccg gcttttgttc gacgccgtcc
     4621 gtgccgccgc ggaggacggg cgttccctgc gggaggtatt tcaggaggtg ggcggccagc
     4681 tgcagcgcaa gcccaactcc atccgcaact attactatgc ccgggtgcgg gaaacgccgg
     4741 atctttctcc ccggcccacg cccttccgcg cctttacccc ggaagagctg cataccctgc
     4801 tgcgggaagt gctcatgggc cggggacagg gggaaagtgt ccgcgcctgc gtcacccggc
     4861 tttccggggg cgaccgttcc caaatgctgc ggatgcagaa caagtatcgt tctgtgctca
     4921 aaaaccgccc cgagctgttg atggaggcgg cggaagaact gcggcaggag gggctgccct
     4981 gcccggagaa ggtggtggtt ttccgccggt acggccagcc tgtgccggga aacggcgaat
     5041 tgggcgcgct caatcgtcat cagagcgacc cggaggtgcg gcggatgctg gacgcgctga
     5101 atcatcttct ggaacagcgg gacagcgccc cacctcccac ccccgcttcg gaggaagccc
     5161 ggcttcacgc caaatggctg gaggcccggc aggaggccga ccggctcaag gtggaggtcg
     5221 atctgctgaa gatggcgctg gaagacgaac gcagcgcccg ttccacccct gcggagtaag
     5281 ctccgcaccg ctttttcccg cgcattgtat atggaaaaat cgccgttgat atgctacaat
     5341 aggagcagta tcaacggcga ttgatttctt cacggaggat gactcatgga cgaaagcacg
     5401 atccgcgaaa aactgtcccg catcaagtgt tttatgctgg acatggacgg gaccttctat
     5461 ctgggcgcca atcttctgga cggttcgctg gactttctgg aaaagctgcg ggagactggc
     5521 cggacgaatc tctttctgac caacaattcc agcaagtccg ccgaggtata ccggcagaag
     5581 ctggcccgta tgggcgtggc ggagcgctac cgcaacgtgc tcacctccgg gcaggcggcg
     5641 ggcagctggt gtctgcggga aatgcccggc aaaaaggcgt ttcttctggg caacgatatg
     5701 cttcgggccg agctggaaca gatgggcgtg gtcatcgaca acgcccgccc ggactacgtt
     5761 cttatcgcct acgacaccac gctggactat gccaaaatga ccgccgtctg cgactttgtc
     5821 cgggcgggtc tgccttacat cgccacccat cccgacttca actgccccac agagacgggc
     5881 ttcgccccgg acatcggcgc gatcatcgcc tttatcgagg ccagcacggg acgccgcccc
     5941 gatctgatca tcggcaagcc ctacggcggc atcgtggagg aagccctgaa ggccaccggc
     6001 cttcagaagg acgagctggc catggtaggc gaccgcttgt acaccgacat cgccaccggc
     6061 cgcaatttcg gcattttgag cattctggtg ctcaccggcg aagccaccct gcaggacgtg
     6121 gaaaaaagcg acgtcaagcc cgacctgatc ttcgaccgcc tttcctccat gaaccccttt
     6181 ttgcaggggt agtacccctg caccccggac tgcgcgcctg aggcgcgcag gggcgtagag
     6241 ggccatgcag cttggccggg gttttaccgc cgtggcacga caggtgccac ggctcgcggg
     6301 tctggtgtgg atccctgttt attgtcgtgt aaagaccgcc attcggcgac gggcctcatg
     6361 gctcacaaaa caggccagcg tgattttagt ttcacgctgg cctgttgcgt tgcaaaaata
     6421 cccaagccac aagaaagcat cccaccccgc aaggggtaag cctg
//
LOCUS       DAWAEA010000032         6413 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_1906_length_6413_cov_3.551903, whole genome shotgun sequence.
ACCESSION   DAWAEA010000032 DAWAEA010000000
VERSION     DAWAEA010000032.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 6413)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6413)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6413
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1906_length_6413_cov_3.551903"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1207 a         2007 c         1947 g         1252 t
ORIGIN      
        1 gttctctttc cagaactacc ccgccatccg gggcaagtcg ctggacatga accagtggac
       61 gctcattaac ttcttcggcc agcaggacta tgagttcctg accaaccggg gcaatctgaa
      121 gacccgcgta ggctctatct ccggcctgtt cgccaatgcg gacacggtgg gcggcatccg
      181 gctcagcttc gtgctttccg ctatcgcggc ggcgctggcc tgcgtcatcg tggtagtggc
      241 ggtgaactac atcttcaaga acaagggcaa gaagcgttcc acggtgctgg aatacagcct
      301 gctgttcccg tggctgctgc ccaccatcct gatctgctat tcctaccgca cctatttcaa
      361 ctccgacagc gtatggtatg tgttcaacaa caacctgtac tggaaggaga acgtgcggtt
      421 cctgatcgtc atggcctaca cggtggtgaa gctgcccttc gccctgcgca tgatcaaggc
      481 ggccttctat gccattgacg aggagctgga ggacgcggcc cggaatctgg gggccagtcc
      541 gctggtcacc ttcctgagag acaagctgcc tatcgtgctg cccagcgtac tggcggtatt
      601 cgcgctgaac ttcaacgccc tgttcaccga gtacgacatg agcgccacct tccattccag
      661 ctacggcaag agctacgcca tggtcatcca gaacatgtgc gccgaggaag gccgggacgg
      721 ctacaacgtg aacgcctccg gccgtcgctg tgcgtcgacg gtgttcatca tgatcgtcag
      781 cggcttgatc ctgtatctgg tctacggcgt cggcgcccgc gatctgggcg accggctggc
      841 catcaaggaa aagcgccgca agcgctggga gaaattctcc ggccatttca aaaaagaaaa
      901 gaagatcgct gcttaaggcg ggctactgaa aaagggaggt gcagaaatgc gcctcccctt
      961 ttttcacccc ctttttcagg aattttcccc ttcctcggtt ttcagaaatt tgcgcatcat
     1021 atagccggta aattgcagat aggccacttt ccaccattcc atgttggtct tttcccggac
     1081 ggcgacccgc tggcccagcg gtaccttggc cagaaccgcc gctttcagcg agggcttttt
     1141 gcggagattc accgtgctgc cgctttccgc cgttacctgt gcgcaggtct cgcccgtctc
     1201 cgggtcagcg ggggagtaat cgacggcttt cagatacccg gcccactgcc agcgggacag
     1261 ccggtcgtcg atccgcacct tgggcggggt ggcgttgatg atctgaagcg gctctacgct
     1321 gaccaccagc ccgatatggt agaaatcctc cgtcagattc gggtcgtaat acgcgccgcc
     1381 gggcttgtat tcgcccttca tccgactcag gtcggagcgg cttttgaaaa tcgccatgcc
     1441 cggttccagc ctggtggagg cggtcacacg cttcatctcc cggcagtaga tgcgggcgat
     1501 ccggttggag ccgtggtaga tacgctcccc aaactggcgg taggcgtaca caaacgcccc
     1561 ggagcagtcg atccccttgg cgttgctggt gccgggagtg acatagggcc agcccagcat
     1621 ggagtgaaac acctgaatca attttcgaac gggaatcacg gagcctcaca tcccgcctga
     1681 tccgcgtagt gggcataggc cctcaggacg aaatgccggg gccgtccggg acggctccct
     1741 tcttcgggca gaatgaaccg aatgggcggg gcgtccacgg cgtgagcagc gccgtcgtgg
     1801 tgggcgggca gggtaagggc gcgaaacccg cgcagctcct ccgcacctcc ttccaccacc
     1861 tcatgggcgc tcagacccac gaagacccgc cgccccgggc acacattctg cagggtcata
     1921 gccacgtcta gcatcctgcc ttcgctgtcg ggcaatgttt cggtgattcg aacgcttttg
     1981 atggattcgc agggttcaaa ataaagatgg gtctccccgg cgggcgtttc ctcggtacag
     2041 tggccgatgc gaacggccgc gccctctccc attcccgcgc cgacgggctg aaaatgcaga
     2101 tcattcatgg aatctcttcc tcctgaaatt caaaggtcag gcacgccgaa aaaagcggcg
     2161 ttccattgcc agcctatgcg cggcggacgg aagggtgcgg cataaagcac cttgcaggac
     2221 gcatagtctg ccccaaaggg ggaagagatc atgacggaac ttcatcaaaa atttctgctg
     2281 ctgcgcccca tgcggacggg ggtcagcggc tttgcccgaa tgcagtggga acggggacag
     2341 gcatgggtgc agctgaacgt ccggggcgcg cccggtgagg gcattcgggc gttctggtat
     2401 tccagcggcg gcgaagcccg ggaactgggg acctgcgccg ccaacgcccg gggcgaggct
     2461 tcgctggaag gctccgtccc cggcgaccgt tacgcgccgg aacggctgca ggctctgatc
     2521 gtggtggaaa acaacgtcgc gccccggccc ctgctcatcg gcctgtgcac cgagaccagc
     2581 gccgggagcc tgctggacgc gaaaaacgcc agtctgtccc tgtgcgacaa gctggcacgg
     2641 gcggcggtca aacgccgtca gccggtcgaa ccgaaaccga cggacgctcc cgtccagcgc
     2701 aaggaaacat tggagagtcg cacgccaaca gccgcccaag cgccggtttt cgcgcctgat
     2761 gcgtcctcca agcccgtgtc cgccccaacc ccagcgcggg aaaaatccct tccgcgggaa
     2821 atttttctcc cagcgctgga ccctgcgccc tacgtgcagg ccatggagcg gcctatgaaa
     2881 gccgcgccgt ctccccggca ggacagcgtt gcgccgactt ccgccgtacc tcccccgcag
     2941 ccggagccga ccaacgaaaa aaagccgtcc cgcgcgggcg tacccgtggg acggcttccc
     3001 aatctggaat ggccgaaagc gtttcgatcg cttcgggagt attttgtcaa atgcccgccg
     3061 gacgcgccct tcgactggcc gggctggcgg ttcgtgcggg tgtcccggca gggggacggg
     3121 ctgtggatcg gtcgtcggat caaagacagc cgggtctgtc gagtagccta cgccatgcgg
     3181 ggcgcaccgc cccgggatga ctcccggccc tatcagccgg tgcggggccg gaacggcgag
     3241 ctttaccgtg tcctggtgca gcggacgtag gaccactccg caccattcgt cagcggcgtt
     3301 ggtaatttct tttttatcac accccattac atcaggcgaa aatttcattc gccccacatc
     3361 cccttatggt atgctcattc cgcctgatga gacaggatcc agtcgcacag ctccttgggg
     3421 ctgcggaaca gatgcctggc cccggcgttc ttcaagtgtt cctccgagcg gaaaccccac
     3481 agcacggcgg cgcagtccac gcccgcgttg cgggccgtct gcacgtccac gtcgctgtcg
     3541 cccacataga gagtgtgaca cttggcgctg ccaaattcct tcatcacatg cagcaccgtg
     3601 tccggctccg gcttgcggcg gatgccctcc cgctcgccca cagccagcgg aatgctgggg
     3661 aaataatact ggcacagggc cttgacattg gggtcgttct tgttggagac caccgccagc
     3721 ttgaggccct tttcctgcag cgcgtcgatc agctcgggaa tgcccggata gggcgcggtg
     3781 gtatcgttat tgtggagaaa gtagtaccgc ttgaaggcct gcagcgtcat ttcgtaggcc
     3841 tccggatccg cgtcgcattc cggcggcagc gcccggcgga tcagcatccc gatgccattg
     3901 cccacgaagc ccttcacgtc ctccatggta tgctccgggt atccgcactc ccgcagcgcc
     3961 tgattggtgg cgttggtcag atcctccagc gtattcagca gtgttccgtc cagatcaaag
     4021 atcacagtgg aatagctctt catcagaaca tccctttctt cccttggcgt ttgatggtaa
     4081 aacaaaagcg gaggtgcagg aggcactgct gccactcaat cgtcgcaggg actactgccg
     4141 tagtccctgc tcgtttcgtg tcacacctgc gggtcgctaa ccgcctacgg cggttgtgcc
     4201 cactgggcga cggtgaactt ttcaaattct atttgaaaag ttcagtctgc caaaggcaga
     4261 ccgggttgcc caagggcaac ccgccttgcg ccttccctgc ggtgcctgcc ggggttttag
     4321 ggcggcgaga aggagcgggc atccagtgga tgcaactgcc gaaggcagtt agcgactgac
     4381 agccgtcaac cgaaacgagg atttggcacg gcagtgccaa agacgacgat tgaggttgcg
     4441 gaacggagac tcagtgcctt atgccgcagc actttccccg cgaaccagct taagggtgcg
     4501 cagcaccgag gacgaggaca gccctgaaag ggctaccgca gtccgaagcc cgcgccccgt
     4561 aggggagcgg gccgcagagc gctgttcgcg ggcatctgca cgccatccgc atttttgaat
     4621 catcgtccca aaggaaaata tctttgcctg cccttagaac aggcagatat actttctcca
     4681 ttttcggaaa aaatcctgcc ttattttttc atgcgggaaa gccgccgtcc acgcccagca
     4741 cctgcccggt gacaaaggcg gactccgggc cggaaagcca cagtacggct cgggccacgt
     4801 cctccggggt gcccagacgg cccaaagggg tctcgtcggc cagctcggcc agatcgtccg
     4861 aggtgaagga gttgagcata tccgtgcgga tcacgccggg gcagacgcag ttcacccgaa
     4921 cgccgctggg gcccgcttcc ttggcaaggc cgcgggtcag cccgatcagc cctgccttga
     4981 cggcggagta aggcacctcg cagctggcgc cgatgcggcc ccaaatgctg gaaatattca
     5041 cgatggagcc ttcccgccgg gagatcatcc ccggcagcac ggcccggcac aggtaaaacg
     5101 cgccgctcag gttggtatcc accatgcgcc gccactgctg gtcggtcacg tcggtcaaaa
     5161 gctcctgcga ggcgatgccc gcgttattga ccagcgcgtc cacgtggggc agacaggctc
     5221 ccgcctcctg caccgcccgg cggacggctt cgctgtcggc cacgtcgcac tgcagggcga
     5281 tggcgcccgt ttcccgggcc agtgcttcgg cagcttcccg gctctgacgg tagaagaacg
     5341 ccgtctgcca acccgcctgc cggaaggccc gcacgcaggc tgcgccaata ccccggcttc
     5401 ccccggtgat cagcgccgtt ttcatgcgga cgcgcctgcc ttttgcgcct cggcagccgc
     5461 accgggcttg ggttcgtgga actcgcagcc ctcgttgtag tagaccttct ccggcggctg
     5521 ttccagttcg tagggattgg tcagacagtg cttcattacg gcgaagagct tggcgtagat
     5581 cgcgccgctg cacacgcgct cgtcggcggt gatgccgatg ggcaggatgc acttgcggct
     5641 gatctcgccc ttcatgtccg gctgattgct ccgctggggc gtgcccatag agaaaaacag
     5701 actggtattg ccgaagttgt agatgtggtg gtacacccgg gtcatgccga tggaggccat
     5761 gttggtcaca tacatgctgg tatggaaggg cagctcgtcc agcagcgcct tgggggccag
     5821 cccatagcga tccagcagct tcaccagtcc taccacggcg ttgggcacca gcggcagctt
     5881 cagcacagcc ttggccagct taatggcgaa gctggggtcg tccgccttgc ggcctttttc
     5941 aatggtctcc ttcacccggg cgctcacgtc gaaaatggtg tcgctggggt cgaagcgaat
     6001 cttgacggcg ttttcctccg cgctgccgtc gggcgtgtcc atcagcaggg taaagctgac
     6061 ggtcagctcg gtgcggttgt agaactgctt gttcatgata aaacgattgg tctccggcac
     6121 ctgcgacacg ccccgcacat aggacgcgat cagcagctcc ataaaggtga tcttatgccc
     6181 ttcccgggct ttttcggcga tgtagcgcat gaggggttca tagtccgcgt cgtgggagag
     6241 aaacacctga gcgtcgcaac gctggggcat cagatagggg gtgatggcaa cgatggggtc
     6301 gacgtcgtga atgcggcggc cctccgggcg gaaaccaaac atgggcatct ttcctttctt
     6361 ttcgggggag aatgaatgca aatgataaat gagaatgaag gcctttccaa ggc
//
LOCUS       DAWAEA010000033         6403 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_1911_length_6403_cov_2.283869, whole genome shotgun sequence.
ACCESSION   DAWAEA010000033 DAWAEA010000000
VERSION     DAWAEA010000033.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6403)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1911_length_6403_cov_2.283869"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4870..4879
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5952..6158
                     /estimated_length=207
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1289 a         1706 c         1855 g         1336 t
ORIGIN      
        1 ttcccttcgt tcccctttgg aaaccttcgg gcaccgaagg tggcgagaaa acccatggga
       61 cggcaagtcc cactcccgcg tcgaagtcgg cgcgccagac ccatgcaagg ggccgcttcg
      121 aagcatcgtt tttccggggc caataagccc catggagtct ctgcccccat tccttgacat
      181 tcccttattc ggggccccaa ctccccgatg ccgaatcgct ttcttttcct tcttttcttt
      241 cgcagccgaa agcaaaagga gtcccccctc cttcaggctg aatcctgccc catcctggta
      301 cgtcttttcc gtgggaaatc cgcgcctgcg aaaaagatcg gggacgacct tccgttcagg
      361 attttccttg tttttttcct gaattgacct gtcgtgattc ggccttcctg cggcgcagcg
      421 ggaatcccgc tgcgccgtgg gtaaatctga catacgtaag ccaaaaccct tggaataaaa
      481 cactttcgtc agtggaaaaa ttgaatttcg ggagagatca aaaatgacta gaacgccttt
      541 gaaggagcgc tctctgcccg gctatacccg taaagaggaa atcgccaata tgacgaccca
      601 catcgtggga gccggcgtgg gcgtgatcgt gtttgccctg tccatctgga tcgcctttgt
      661 tcatcagaat gtgtggagcc ttgtgtccgg cgcggtgtac ggcctgagcc tgatcctgct
      721 gtatacggtt tccagcgtgt atcacggcct gattccctcg ctggggaaga aggtgatgca
      781 ggtcatcgac cattgcacca tctataccct gatcgccggg acgtatacgc ccgtggtgct
      841 gtgcgccatc cggcccgtct acccccggct ggcgtgggcg ctgctggcgg cggagtgggg
      901 cacggcggca gtggcgacgg tgttttctgc catcgaccat aaaaagttcc acgcggtgga
      961 aatgaccgcc tatctggtga tgggctggct gtgcgtggcg gcctaccgcc cggtggtgga
     1021 atgtttgagc cgggagggct tcgcgtggct gctggccgga ggcgtggcct ataccatcgg
     1081 cgcggtgctc tatggcctcg gcagtaagaa aaatcccatt tttcacacgg ttttccacgt
     1141 gtttgtggat atcggcagca ttctgcacgc cgtgtgcgtg ctgtgctacg tcctctgacg
     1201 gcggtttgac acccggcatc ggcaatgata caatagccct agcgaaaata cgcgaatttt
     1261 ctgcaaagga aaagagggaa cgctatgacc attgccgtgc tgatcccctg ctataatgaa
     1321 gcgaaaacca tcgccaaggt ggtcagggat taccgcgccg ccctgccgga agcggatatt
     1381 tacgtctatg ataataactc caccgaccat accgacgaca tcgcccggga ggcgggggcc
     1441 attgtgcgct acgagtaccg tcagggaaag ggcaacgtga tccgctccat gttccgggag
     1501 atcgacgcgg actgctacct gatgatcgac ggcgacgaca cctacccggc ggaaaacgcc
     1561 cgggaaatgg tgaatctggt gctggaacgc aaggtggaca tggtggtagg cgaccgtctg
     1621 tccgccactt attatacgga gaataagcgt cccttccata atatgggcaa ctggctggta
     1681 aaggggctgg tgaataccat cttcaagggc agcgtcagcg atatcatgac cggctaccgg
     1741 gcgttcagct atcagttcgt caagtccttc cccgtgctga gcaaggggtt tgagatcgag
     1801 acggaaatga ccattcacgc gctggataag aatctgagcg tccagagtgt gcaggtggaa
     1861 taccgggatc gtccggcgga cagtccctcc aagctgaaca cctactccga cggcatgaag
     1921 gtgctgcgca ctatcgtgcg gctgtataag gaataccggc ccatgagctt cttcgggctg
     1981 ctggccgcgc tgatggcgct ggtgagcgtc attctgttca tccccatttt tgccgaatac
     2041 ctgcataccg ggctggtgcc ccgtttcccc acgctgatcg tgtgcggggt gatggggacg
     2101 atggcgctgc ttttgtgggt gtgcgggctg attctggaca ccgtggccaa gaagcatcgc
     2161 cagctgtttg agatcaacct gaacctgctc aacggccagc gccggaggga acgggtatga
     2221 cggcgccccg gacgaacgaa aacagaaacg gactccgctt ccgaaacgcg gccctttgcg
     2281 ccgcgtttgg cgcgttgagc gccatgagct ttattgtcag cggagaaaac tatcagggta
     2341 tcagcgaccc gcccgccagc agcgccagct acctgtggtg cgcgctggct ctttgctttg
     2401 ccctgctgta tcacaccgtg tacgggcgca ggaagctccg ccccgcgccg gcgaacgggg
     2461 cgttcgccct gatcttcggc gtggtaaacg ccttcggcgt gtggctgtac agctatgata
     2521 gctgggcggc gctgaaaaat cccctgacgc tggggctggt gtgtctgcag gctgtggggc
     2581 agtctttgcc catgctggca ggcttcacat ggctccatga ctggatgcag aagaccttcg
     2641 cggcgctgcc cgctgtggag gaagcaccgg ccaaggcacg ctggtattgc cgtcacccgg
     2701 tactggggag catggcggcg ctgctggtgt gctggtcgcc cttcctgatc gcctttttcc
     2761 ccggttccgt ctgctgggat ctgggcgaaa tggcggcgca gtacttcggt ctgcggcaga
     2821 tcaatacctg gcacccggtg ttcctgacgg ggctgtacgg ggcgcttctg agcttcggac
     2881 ggctgttttc cagcgataat ctgggcacag ccctgtatat gctgctgcaa acgctggcct
     2941 tgagctatgc cttcgcccgg acgatggagc tgctgcggcg ctggggactg ccccgctggt
     3001 tccggctggc ggcgctgggc tttttcggtc tgacacctct cttcggcggc tacgcccagt
     3061 ccatctgcaa ggataccttc tacacggcgt ttctgctgct gttcgcgctg aacgccatgg
     3121 aagtgctggc gggccgggag cagggaaaat ccccgtcccc tgcggcgctg gccgggctgt
     3181 tcggctgggg actgctcagc tgtctggttc gctccaacgg gctgtatgtg gtgctccctt
     3241 cggggcttct gctgctggct gtgggggcga agggcaaggc tcgtctgccg ctggccgcgt
     3301 cgctgggcgg ctgcgtggcc tgcgctctgc tggtttccaa cgtgctgatc ccggcgctgg
     3361 gcatcccgga cgaaacggcc tcgggcattt attccgtgtg cttccagcag agcgcccggg
     3421 tgctgcggga tcacggcgat accgtcaccc cggaggaata tgccgaaatc gaccgggtgc
     3481 tggacgcgga aaagctgccg gaactctacg agaccaacat ttccgacccg gtgaagttca
     3541 ccttccgcta ttccgggcag ggtgcagccg tggaaaaaga agccctgaag cgctatgccc
     3601 agacgtggaa gaagatggcc gcagaatatc ccttaagcta tgcggaagcc ttcttcggcg
     3661 gcagcacggg ctattacgcc ttcatcccca aaatggaggg ccccagctac aacaatcagg
     3721 ccggcaaccg gctgatgttc gaaacctata ccttggggga cgatcccggc tacgtccaca
     3781 cccgccagat cggcttcctg tccaaggtgc gaacactgct ggccgcctat gccaggggct
     3841 ggcggcggat tccgctgctc agtctgctgt actgctgcgc aacgtatacg tggctgctgg
     3901 tgggcgtggg tctgtatctg gcccgccggg ggcagtggcg gcggctggcc gcgttcctgc
     3961 cggcgctgct gagtttgggc gtatgtatgc tcagcccggt gaacgactac ttccgttatt
     4021 tcctgccgat tgtggccatg gccttcccat tgacaggctt tgccctgcgg ccaaaagaaa
     4081 actgaaaccg acggcccgct tcccataagg aggcgggctg ttttgatgga gcgaaaatac
     4141 ggctacagtt tgagaacatt cagaaggagc tggcagaggg aaaacaatgc ggttttcagt
     4201 gcggcggggg gtcaagaccc tcgctgcact ttgtacagcc gtattcctgc cggctaacct
     4261 caaaataccc tgtgaggctt tgccgtgtgc agtgcccggt cggatatgga caaacaagga
     4321 aagggacgta gtccgaaacc tgcgcttaat ggagaacata ggctgcgctg gaaatgactg
     4381 aaagagatga agcgcatagc caagtgcgga gttaggccgc tgggcaaaac ggagcgggcc
     4441 gtaaatttcc gacgttaaaa agcgccgtaa atgcgctttt ttgacaagcc gtgttagccg
     4501 ctttagaacg accgttctct ggaacgtcgt ttttcgacat aggactcgtt tgggcggacg
     4561 actgaatctt agcgaaaaaa agacgcaatt ctaacattat tttcatgttt cgaagtattt
     4621 ttgttgtttc tgtgcattgt ttcttcacga gaggatatag gaagactgga taaacccaaa
     4681 taattcaagc gtttttgaaa tgggaaatac gtgccgatat aaacaacaga aaacggcatt
     4741 ttgtaccatt ttggttcttt ttacttttgt tacaaattcg atttgcggaa aaatccacgg
     4801 ttttccggga aaaagacgca gcaatcaggc catttaccga ggttttgaca tgatcggtca
     4861 ggttcgcagn nnnnnnnnnt ttcgggaatg gcgcgcttct gcatggtact gtgtcacaat
     4921 gtaaaataat gattctattt tgcacgaaaa cgacaaatca agagaaactc gtgctatact
     4981 ctgaaaaaac aggggaaagt tacaaaaccc gcaaggattt taggaaatgg aggaacgagg
     5041 gatggaaggt tatcgaattc tgatcgtgga agatcacgtg cagtcacgca caacgatggc
     5101 ggagtacctg cgcaggcaag agggcgtcgc gctggtacgg gaggccggaa acggcgcaga
     5161 agccctcaaa ctgctgagcg aagaaccgtt tgagattatg atcaccgaca tcatcatgcc
     5221 tcaaatggat ggctacgcgc tgctggaaga aatgcgccgc agggaacttc cgtccaaacc
     5281 ccagaccatt gtggtgtccg cgctgggacg ggatgatttc gtcacccggg ctattgagct
     5341 gggagcgacc ttctacatgg tcaagccctt cgatatgcgc catctgatga gccacatccg
     5401 cgatttgtcc ggcggaagga tcagaccggc ggatgccgca cccactccga gagagacaca
     5461 ccgggtgcaa acgctggacg agcggttggg cagcctgttc ctcaccattg gcattcccgc
     5521 ccatatcaaa ggctaccaat tcctgcgtca ggccgtcaaa atggtggtgg acgagccgga
     5581 tatgatcaac tgcatcacca aggaactcta ccccggcatt gcccggcact tcggcacgtc
     5641 tgccagcaaa gtggagcggg ccatccgcca cgccatcgaa gtggcgtgga accggggccg
     5701 gatcgagacc ctgaacaccg cctttggctg caaggtctgc accccggagg acaagcccac
     5761 caacggcgaa ttcatcgcca tgatcgcgga taaactctct atggagcgca gcgcgtaaag
     5821 aaacgcaaca aaattccgca gcgcagcaga gcgcaaaaat gcggcaggta aaaatggtaa
     5881 agaggccggc gccattcctg agaaaaaacc gtcagggcag gcagcgggcc tctttggtat
     5941 agaaaatgaa annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt ttgacctcgt ccagttgact
     6181 ttgactaccc gttaccgccg cttcgggctg gacggcggcg gatgcgcccg tgatggaatc
     6241 cacagggatg gataagacgg cacatacggt cgctaccgtg tcgaaagctg gcgtagtgcc
     6301 ccggttcaaa aagccatcca gcgtactttt tgggattccg gcggcgtcgc tcagattctg
     6361 aacggtcatt cgctgggcgg aaagggctga tttgatacgg ctt
//
LOCUS       DAWAEA010000034         6270 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_1960_length_6270_cov_2.251327, whole genome shotgun sequence.
ACCESSION   DAWAEA010000034 DAWAEA010000000
VERSION     DAWAEA010000034.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6270)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1960_length_6270_cov_2.251327"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2467..2476
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5448..5457
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1159 a         2018 c         1824 g         1249 t
ORIGIN      
        1 cgatcgccca gcttcacccg gagcacgccc agcgcgtccc ccgctttcag aggcgcttcc
       61 acttcttcgg gaagctccac ttccaagctg agctgttttt cctgccccag ctttatcagc
      121 atactcagac ctgaacccgc cgccgcgtcc acctcgtcct gcattcccag atggactttc
      181 accttctggc ccagccgatc gcccttctgc agcaccgtca cccgtttgta gccgttaaag
      241 ccgtaatcca gcatggcccg cgcttcgttg aaccgcgtct ggctggcgga cgcgcccagc
      301 accacggcga tcagcctcat gccgtttttc tccgccgtgc agctcaggca gtatttggag
      361 tcatccgcgc tgcctgtttt cagaccgtcg cagccgtcgt agaaccgcac cagccggttg
      421 gtgttggtca ggtcggtgac ccggccgctg gggtgggtca acgtgtccat ccacaccgcg
      481 ccataggtga aatattccgg gtgggtgcac atttggcagg aaagcagcgc cacgtcccgg
      541 gcggtggtgt gctgaccctc taccggcaga cccgtgcagt tgacgtacac ggtgtccgtc
      601 atgccaagct cctgcgcccg ctcgttcatc cgggcgacga aatccgtttc cgtgcccgcc
      661 agatattcgg ccagcgcata ggcagaatca ttggcgctgg cgataatggt ggatttgagc
      721 aactccttga gagaatagac cgctcccgcg tccagcagtg cctgactgcc gatctgcccg
      781 gcagcggccc tgctgaccgt cacctgatcc tccagcgaga ccgttccttc ccccagcgct
      841 tcaaaggcca gcagccgcgt catcagctta gtgacactgg ccacggggcg ctgctcgtcc
      901 gcattcttct cgaagatcac cgtgccagtc tccgcctcca ccagcatggc ggaaggcgcg
      961 gtgatctcca ccgggaccgt ttcttccatg ggagccgtcc gcaccgccgc cgtgccgagg
     1021 ggcacaaggc ccagcgccag cagcagcgca cacaggcgct ttcctcgatg tccgatcata
     1081 tcaaaaagct ccttccgttt ctatcctgaa aacagcatat ccacggaagg agtaaaataa
     1141 accagtgtga aaaattggga attacagcgc ttcgatcacg ccatccacca acgcccggaa
     1201 ggtgtgcttc acccggcggc ccacttccag cacttcttcc tcggtgaggg ccttatccag
     1261 aatgcccgcc gccatattgg tgatgcagct cagacccagc acgtccatgc cgctgtgccg
     1321 cgccacgatg atctccggca cggtggacat ccccaccgcg tccgcgccca gcgtgcggat
     1381 catgcgaatt tcagcggggc tttcatagca ggggccgttc atccagcaat acacgccctt
     1441 atgcagcttg atattctgct tttccgccac ctgcgcagcc agctcctgca ggctgcgggt
     1501 catagcgtag gtcatgtcgg ggaagcgcac accgaacgcg tccagattgg ggcctgtcag
     1561 cggatttttg ccgctgaggt tgatgaaatc gctgatgacc atcagcgcgc cctcgccgaa
     1621 gtccagattc acaccgcccg ccgcgttggt aagaatgacg gtcttcacgc ccagcagctt
     1681 catcacgcgg atgggcatgg tcacctgcca ctgttcgtag ccctcgtagg aatgaaagcg
     1741 tccctgcatc atgcagacct ttttgccgtg cagcgtgccg cagtgccagc ggcccgcgtg
     1801 ccccggcgcg gtggataccg ggaagccggg aatatccttg taggcgatgc tcttggcgtt
     1861 ttccaggctt tccacatagt cgcccagacc gctgcccagc accacgccga tctccgcccg
     1921 gccgcaggcg gcttcgatcg tgtcggcggc agtttgcaga gtcttcatat attcttcagg
     1981 ggtcattttc atttctcctt tcattttggg gaaggggacg gcgaaacgct gcccctccgt
     2041 caaggccgtt attcctgacc agccgtttta ctcacagctt ttccagaaag ctgacggcgt
     2101 tgaaacgctc gggaacgccc agatattccg ccaccgtagc ggccatatcg gcgtaagtgg
     2161 cccgggtgcc caaatcggtc agccccttca tgcccttgtg ccagaccaga atcggcacat
     2221 attcgcgggt atgatcggtg ccggtatagg tggggtcgca gccgtggtcg gcggtcagga
     2281 tcagcaggtc gtcatccgtc atcttcgcct gaatttccgg cagacggctg tcgaaatact
     2341 ccaatccctc ggcatagccc ttcacgtccc ggcggtggcc gtacacggag tcaaagtcca
     2401 ccagattgac aaagatcagc ccggaagcgt ccctatccat ggccttgagg gccgcgtcca
     2461 cacaggnnnn nnnnnngtga atacaccgtg agctttcaat acgtccagca gtgtttcccc
     2521 ggtgggttcc agcgagaaat cccggcggcg gggcgtgcgg gtgaagtggc ccttgcccgt
     2581 gccggtgaag gggcgcgcaa tgacccggcc cacggcatgg tcgcccacca gcatttcccg
     2641 ggcaatgcgg cagatgcggt acagctcttc cacggggatc acgtcctcat ggcaggcgat
     2701 ctgaaacacg ctgtcaccgg aggtatacac gatagggcag ccggtgcgca tatgttcttc
     2761 gcccagttcg tccagaatgg cggtgccgct ggcgggataa ttgcccagtg tcttgcggcc
     2821 gatgcggcgt tcaaactcgt ccattacgtc cttggggaag ccgttgggat aggtagggaa
     2881 cgccttgtcc agcttcaggc ccatcatttc ccagtggccg gtggtggtat ccttacctgc
     2941 ggagacctcc atggcccgac cgaacgcgcc cgccgttttc tccggcacgg gatagcccag
     3001 cccttcgatc cggccaaggc ccattttttc catataaggc aggtcaggat gcgcctgttc
     3061 ccagatatgg cccagcgtat tcgcccccac gtcgccgtac ttgtccgcgt ccggcagtgc
     3121 gcctgcgccg acgctgtcca gaacgatcat aatcacgcgc ttcattgcct gttcctttct
     3181 gacggctaaa gccgtctcaa gcattgtttt cgggttttca cccgtttttg tccctttgat
     3241 tatactgttg aagaaaagtc cccgtcaagc cccgctgctg cgggcagcca ccatgggcgc
     3301 ttctcccggc ttcgcctccc ccagcagggc gcggtaacgc tcgtccagca gcgccgggtc
     3361 aaagcccgtt aaaaacagca gatgtttttt cggcatggga tgcagacaga accagttcgg
     3421 gcattgatgg atcagccgct gccagagtgt cacctccgaa aagccccgct tgagttcgtc
     3481 gtccacctgc gttagattca ccgtcaggcg gcagagaggt ttcagcagtt cgaccggaac
     3541 catcacaaag gacaggtcgg gaatggcgtt gaccgtggcc agatgaatgc gcccgtccat
     3601 cagcggtgtg aaatccggct cggcctgcgg gcagatggga tgcttgagcg tatccatatt
     3661 cacccacgcg acgctgtcga agtccgggtg ctgttcggcg gtcttgagca gcagcagcgg
     3721 cttggagcgc tgaagccact gttcttgcgt catgccctta ggcagatagc tgcggggcag
     3781 cagactgtta tccgggcagc ttctggcgtt ggggtacagg ctgcgaagcg ttcgttcctg
     3841 cgtgccgccg gtatagaggt acaacggcag cgcttccagc gccatcagga aacggaagcg
     3901 cagcagatag ttcatgatgg gcttgcgccg ctggggcagg tcgatgaagg ccgtcaccag
     3961 aagtggctgg cggatgcgac ggcttttggg cagaaagcgc tgcttctgtt ttttccacaa
     4021 aagggcggct ggcagctgct gggcatacgt ttcctcccgg gtaaacagcc cccaccgcgt
     4081 ccgcaggtcg gagcgccgat cctccggcgg cccttcccgg aaaccggcca actgctcgaa
     4141 acgggccagc gtagtgccgg gcaggacttc ccgcagaggc cgcctcagca gggcgggcgg
     4201 ttcctcccgc agaggccaga gcgtcggcct gtccagcaca tacacgggaa tggccgacgc
     4261 ataggctgcg aaggacagcg tttccggtcg caggtccatc cggcgaagcg tttccgtcct
     4321 tgcgaagatc agcccggggc acaccaccat cgtagggggc ggcgcggcgc tttccaccag
     4381 cggaagcccc cggacgatct tcgcgccttc cgcctcgaac cgttcggaca gacccggcag
     4441 ataggcttgg ggcggatagg ctccgtccgc cgcgccgatc atgccggtca gcagcgcctc
     4501 ccggcgggct gttttggcga agcgccgcaa gagctcctga tcccacttgg ggccgaagtc
     4561 atattccccg gccagcagca gcgtatgact ggcgctatcc tccagcgccc cggcgaagcc
     4621 ttcccgctcg ccgtaaaaat gcacctgagc ctgcagcccc atccgaggcc acacgtccca
     4681 aaattccttt tccagtctac gggacaaaga aaaccgcagc cccgcaggca cttccgccgc
     4741 ccgcatggcg cggctcacgg tcagcgccgc cagttccgcg tcgggtattt tccccaaagc
     4801 caccagaatg ctggatatgg gcatgggttc atctctcctg taatggattt caggctttct
     4861 ccacgtactt ttcgatcagc cgcagtgtat tctccacgcc cttcacatgg ctgcgctcat
     4921 aaccatggga ggcgtacacg cccgcgccga tcagggcgta gcggatatcc cgtccggagc
     4981 gaagcgccgc gcccgcgtcg gagccgtaga aggggtacac atccacggcg tagtccagct
     5041 gatgcttctg gcacaggtcg atcagctcgc cggtcatgga atagtcatag gggccggaag
     5101 aatccttggc gcagatggac acctgctgct cggtgcagct gaggccctcg cccacgcagc
     5161 ccatatccac caccagcaga tcctccgtat cctccggaat accggaggac gcgccgtggc
     5221 ccacctcttc gtagacggag aagaacaggg aagttttccg ttcgggctgc acttcgccct
     5281 cggccacggc ccgggccaac tccagcagca ttcccgcgct gagcttatcg tccagaaagc
     5341 ggctgcggat atagccggag ggcgttaccc gcgcccgggg gtccagacag atgacatcgc
     5401 cgttctggat gcccagcgcc agcacgtcct cccggctctg gacgtttnnn nnnnnnnatc
     5461 cagcaaaatc tccagcgtct tgatatcgcg cttgccctct tccagctcgc ggttcacgtg
     5521 aacggaggcg ttctgcattt ccaccacgcc ggtatactga cggccgtccc gggtgacgac
     5581 ggtcacattt tccgtctcca cggcctgaca ggaggggcct cccagcagag caaagcccag
     5641 tcggccattg gctttgatct cccgcaccat caggcccagc gtatctacat gagcagccag
     5701 tgtcagcggg tggccctcgc cgcccagcgt acacaccacg cagcccttgc gggtacgggt
     5761 gggggcgaag cccatttccg tcagctgatt cagcagccag tccgttacat tcgccgtata
     5821 gccggtaggg ctggcgatcg ccagcaggtt catcgcctga tcgacggcgt tttgtgtatt
     5881 catattcaag aactctcctt ttaatatggg gtgatttcag gggggcagca aacagaaggg
     5941 tgcatggagt ccccatgttt cttttcggga taaggggtgg aaaatcctgc cagggtggga
     6001 tgaacctgtg acccaaaggg cataaacggc ccggtgcgat tacggggccg aaggggtgca
     6061 ggggatgagc aggaaggccc ctgcttacgg cgttatatgt taactttgtc tgaaatagtt
     6121 tccctgctgg ggcatgacgc gccgactacg gcgcgaacgg tggggctgcc gccccgaagg
     6181 gggtagtact ttctttcgaa gcgaaagaaa gtaccaaaga aagccagcgg cacggcgact
     6241 ccagaggcgg cccgccccgc tgcgcgttat
//
LOCUS       DAWAEA010000035         6198 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_1989_length_6198_cov_2.572196, whole genome shotgun sequence.
ACCESSION   DAWAEA010000035 DAWAEA010000000
VERSION     DAWAEA010000035.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 6198)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6198)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6198
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1989_length_6198_cov_2.572196"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2440..2449
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5891..5900
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1235 a         1920 c         1917 g         1106 t
ORIGIN      
        1 tatcagcagg tgattgacaa cgccacggaa aaaatgaaga agaccacctc cgtttttcag
       61 acggaactgc ggtccatccg cgccggacgc gccaatccgc agcttttgga tcgcgtaatg
      121 gtggattatt acggtgtgca gacccccctg aaccagatgg gcaacgtgtc ctcgcccgaa
      181 ccccggatgc tggtcatcaa tgtgtgggat cagaaggccc tgccgctggt ggaaaaggcc
      241 atcctgaaga gcgatctggg cctgaacccc tccaacgacg gcaagctgat ccggctggtg
      301 atccccgaac taaacgagga gcgccgcaag gagctgacca aaatggtccg caagagcgca
      361 gaggacgcga aggtggccat tcgctccatc cgccgggacg ccatggagca ggtgaagaag
      421 ctggaaaaag acagccagat caccgaggac gaccgggcca aggccgaaaa gaagattcag
      481 gaaaagaccg acgaggccat caagggcgtc gataccatct gcgctcagaa ggaaaaggag
      541 atcatggagg tctgatgcgc ctgacctttg aacatttgct ggggctgccc gcggaggcgg
      601 ctctggccat cctccacgcc agcggcatca tggatgtgga ggtagttccc accgccgcac
      661 cgccgcgccg ccaccccgtg ccggaggatc agtaccgcac ggaggagggc gaggccgccg
      721 gatatgcctc cacccgcgtg gtcgccgtgg aggaggacgg ccggcggctg atcgtttccc
      781 gctttttgac gggtctgcga tcggaaacag ccaacgaaaa cccgcaggag gaatgaccct
      841 cctgcggatt ttgcctttta cggttctttt tcccggtttt tctcatcctg ccagaccaac
      901 tgctccaaat attccccgct ggtcaggcgg cgcgacgcgg cccgttccag cgcgtggcga
      961 tacgcctccc gatccatctg tacgcagacg ctcaccttgt ccggcgcgca caggccgaag
     1021 ggttcatcca gtccttgggg gtcggtaaaa agtctgctct tcatgggcgc tccctccgct
     1081 gcatcctatg ccgcagacgg cggcgggttc catggggcag tattccccgg cgaaggctgt
     1141 atcatacgga aaaagtcaaa acaggcctgc ttcttttggg agaagcgggc ctgtttgcta
     1201 tgatcgtttt taattccggg cagtgaccag atgcagcagc accttgacgc atttttccat
     1261 ttcgggcacg gagacgtact ccatcaggcc gtggccgttg tagccgccgg tgggcaggtt
     1321 gggacagggc aggccctccc aggaaagccg cgcgccgtcg gtgccgcccc ggatagcgac
     1381 gatctgtggt tcatctccgg cggagcggat ggcgtccagc gcccgctgcg tcacttcggg
     1441 gtaacggtcg atgacctccc gcatgttgcg gtagctgtcc ttcagggtga cgcgcacggt
     1501 gcccgcgccg tacttctggt tcaggtaagc ttcggcggcc agcatcatgg cctccttggt
     1561 ctccagtttg gccgcgtcat gatcccgcag aatgtattgc agcacagcgc cgctttcatc
     1621 gccctgaatc ccgcacaggt gatagaagcc ctcccggccc tccgtgtgct cggggcactc
     1681 ggcggcgggc agcatggcgt tgaactccat ggcgatcttg ctggcgttct tcatcttgtc
     1741 cttggcgctg ccgggatgga tgttgaagcc cttgacctcc accagcgcgg aggaggcgtt
     1801 gaagttttcg cactcgtatt cgcccacgcc gccgccgtcc accgtgtagg cgaagtccgc
     1861 gccgaagccc ttgacgtcaa agtcgtccgc gccctcgccg atttcctcgt cgggggtgaa
     1921 gcccacgcaa attttgccgt gcttcaggtc ggggttggtc aagagggtct cgcacagggt
     1981 catgatctcg gcaatgcccg ccttgtcgtc gccgcccagc agggtggtgc cgtcggtgac
     2041 gatcagctca tggcgcttca ggcattcaaa ttcctccggc ttcatgacca cgccgttgcc
     2101 cacgtccagc tcgccgccgg catacaccag gcagcgggga tgcaccgggc cggtgggcac
     2161 ggcgggggcg gtgtccatat gggcgatcag gccgatggcg ggctgatcgg gcacattggc
     2221 agggatctcc gcgtagacgt agcaggtggg gctgacacga acattctccg cgcccatttc
     2281 cttcagttcc ttttccagca gtcgggccat atcccactgc ttttcggtgg agggatgggt
     2341 tccgctggtc tcgctggagc cggagtcgat gaccacatag ttgagaaacc gttctttcac
     2401 attcatggga cagtacctcc ttgaaaaaat caaagctacn nnnnnnnnng gtgagaacga
     2461 tgttgctttc ccgggccagc agggcatgct tcggcgcggc ggggaaggcc tcgacgctgc
     2521 cgccggagtg gcagagaagc atcatttccg cgaaatccat cccggcggcc atggagaagc
     2581 ccaagccgcc ggaaaagcgg ggattgacct ccagaaagaa gaattcgtcc ccgtgacgga
     2641 tgaactccat gttcaccacg cccaccagcc ccacagcctc cgcaatggcg gcgcagacgg
     2701 cctccagcgg atgaccgggc agcacctcca cggtcatgcc cagcccgctt ttgttgcgca
     2761 gaagctcctg ccgggcggcg gcgcgggtat gaccgaaagc gtcccgggcc acgtccacac
     2821 agaaaatatc cccttccaga aaaggctgca cgatgcagtc ctcccggccc gccagtgccg
     2881 tctggtagct cttttcgtcc gccgccacgg cctgtcccag actggaacgg ccagaccggg
     2941 gtttgaccat caccgggaag ggaaaggggc agggctgccc cggcacggca atctccgtgg
     3001 ggatcaggcg gcagacctgc ttttccgcca ggaaacggtt catcgtgtcc ttgcggcggc
     3061 acagggcggc gacccgctcc tccggcacgc agaccgtaca gcccagcgcc cggaattcgg
     3121 cttttcgagg ggccagcgcg tccacctccg ggtcggtcag ggggatgaga aagtccagcc
     3181 cgttccgacg gacggcctcc ctcagctgct ccacgtaggg ctcgccctcc gtggccagca
     3241 gggcctgaaa aaactccaac tctccggcca gcgccgtcca ttcccgggga taaatatcgc
     3301 agccgaatac ggcgtggccc aaggcctgat agcgccgggc caccggggct gcggccatgg
     3361 aaccgatggc ggtcagcaga atacggctca tgcttgcgtt cctcctttgg acgttccggc
     3421 ggccttgcgg cgcaggcctg cagccgtctg cacaaggcag cggaagctct ccggcttgaa
     3481 cagcaggctc atgccgccgt agaggagtac gcccgccgcc gtctgcacca gcagcagggg
     3541 caggctgccg agaggcagac ggcccagcgc gctgacggcc agaaacatga caaggctcat
     3601 gcctatgggc ggcagaatat cccgaatctg ttcccagtag ccgtaatgga tcagcttttt
     3661 gttggggaag gcgttcacga acgacgagat caccgtggaa accaccgcgc cccacacgat
     3721 gggcagcaca ccctcaaagc agaacacggt gacgcacaga atggcgatgc cgtagctttt
     3781 tttgataatc tccagcttca ggaacacgtc gctgcggccc atggcgttga tggcctgcag
     3841 gttggcggtg tggatggggt aaaaggcgaa gtcgatgcag cagatctgca ggaagggtac
     3901 gcagggcagc catttgtccg tcagaagcag gctcaccagc ggacgggcca ccgccgccag
     3961 ccccgccatc agcggcagca ccagaaagct ggaggccata atggagcggc gcatggtctg
     4021 cttcatccgc tccagatcgt cctgctgctc ggataaaacc ggcagcatca cgctctgaat
     4081 ggagccgttc accgcgttca tcagaagctc cgggaactgc ttgccccggt tgaaatagcc
     4141 cagcgcctcc gaggaaaact tcttgccgat gacaaaggaa cgcaggtgct ggtagcccgt
     4201 atccatcagg ctggacaaca gcagcttcca gccgaaggag aacaggctcc gcacccggcg
     4261 gagggaaaag cagaaatgag gccgccagcg caccagaacg agcagaagga cgcacaggct
     4321 gaactgattg gtcagctgct gccccaccag cgcccatacg cccagcccct tcagggccat
     4381 ggtgatacct acgccgccgg acagcaccgt ggcggccaga ctggccgtca tcagcttgcg
     4441 gaaggacatt tcccgggcaa tgaccgcgtt ctgtacgctg gtcagcgccc cggggaacag
     4501 gatcagcgcc agcacccgca ggtaacttgc cagcccgacc atagcgaaat agcgctcgat
     4561 ggcgggcgcg gcgaagaaca gcgccgtata gagcagcgcc gcaatggcga gccccagaaa
     4621 aaacacggag ctgcggtcgg tctcgtcgat ctcggcccgc tggatgaggg cggtgttcag
     4681 gccgctttgc acaaacacct gcgcaatggc cgtgaagacc aacaacaggg acaaaacgcc
     4741 gtaatcgtcc ggggtcagca gacggcccag caccagactg accacaaagg cgacgccctg
     4801 attgccgaag ctctccagca gcttgaacag gaaggaggaa gccgcggact ggctgcgttt
     4861 ctcgctcata gggtcacgcc tttccgagga tcaggccgca gatgcgctcc acgtcgccgt
     4921 ccgccagttc cgggtacagg ggcagcgtca ccacctgccg ggaggcgcga agcgcaatgg
     4981 gcgtttgagc cgagtcgaag cccggacggt tccgatagca gtcgaagtcc gtaatcagcg
     5041 gataaaaata cttccgggcg tggatatccg ccgccgccag ccgggcgcag atctcgtccc
     5101 ggcttccggc ctgaccggtg aaccgcacgg gataataggc gtagttggac acaacgcccg
     5161 tttgcttctt cgggcagacg atcccttcgt ggccggacaa aaggcggtcg tacagctcca
     5221 ccacatggcg gcgcttttcg atgttttcat ccagatggcg caggttgcac aggcccatgg
     5281 cggcgtggaa ctcgctcatt ttggcgttgc cgccggggta gagcaccgtc tcctcgtcct
     5341 cgatgccgaa gttccgctcc cattccagct gccggttcag ctccgggtcg gcggtggtca
     5401 cgcagcctcc ctccacggtg tggaacacct tggtggcatg aaacgagaac atggccgcgt
     5461 cgcccatccg ggcggcggac acgccgtttc ggctgacgtt gaaggcgtgg gccgcgtcat
     5521 agatcagctt cagcccatgc tttttggcca tggcggacag ggctccgtcg tcgcagaggt
     5581 tgccgtacac atgcaccggc aggatggcgc aggtgcgcgg ggtaatgagc gcctccacct
     5641 tcgcgggatc catggtgtag tcgtcctccc ggatgtcgca gaacaccggg gtcagcccgg
     5701 cccggacgat ggcgtgagtg gtggacgcaa aggtgaaggg cgtagtgatg acctcgccct
     5761 tcagccccag cgccttcaaa agcgcctcca gcgccagatg gccgttggtg aacagcgccg
     5821 cgtagggcac gtccagccgc tccttcagcg ctgcttccag ctttttcagt ttttcgccgt
     5881 tattggtcag nnnnnnnnnn aacgggtcac attgatacgc atagccatag cgcctttctt
     5941 tctgcggccc gtcaaaaggg gcgcggccgc cggaaattcc gctgatagta tatcatggag
     6001 gcgaaagaat ggcaaattgg gaagcaaaaa gagacgctca gcttagaagg tgctccatag
     6061 ggatttttaa tcccggaaaa aagtgcggca tagtcggtag aaatatcggc tatgccgctt
     6121 ccttgttcat tgggaatttc tactgcgcca attgtagacg agccactcgc caatgtggat
     6181 tcggagacag ccgaaaag
//
LOCUS       DAWAEA010000036         6189 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_1997_length_6189_cov_2.931040, whole genome shotgun sequence.
ACCESSION   DAWAEA010000036 DAWAEA010000000
VERSION     DAWAEA010000036.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 6189)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6189)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6189
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1997_length_6189_cov_2.931040"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    310..347
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1199 a         1897 c         1863 g         1192 t
ORIGIN      
        1 aattttcgcg gccaggttct cgttcttttc gatgtgggtc agttccatca acggggtatg
       61 gccgatcagc tggtcggcgg aagtatagat cttagacatg agaatcgctc ctttttcagc
      121 tttgttttga taggggattt ttcgtcaggc aaagggaagc cgacatcgaa aaaccgtctg
      181 ctgggagagc cgcctcatga ccattgctcc ttttattcca tacctacttg gtgggttggt
      241 ggtattataa gacgaaacca aaaacttgtc aaccgatttt ggcaaaaaaa tcgtcccttc
      301 cgtagaaaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtc ttgttattcg
      361 cagtcctcgc agccggtctc gtgctggaag gccgcgtagg ccgcattcag gcacagccgc
      421 aggaagcgtt cgccctcggc ccggttggcg ggggtgtacg cgccgttcat caggatcacg
      481 gtgaagaagc catccagcgg gctgaccgca agggcgctgc cgctggcggg atcccgcaga
      541 ccgtagccgt cgctgggcca cagcagcccc agaaacggat tctgccgctt agtgatgtgg
      601 aaaccgtagc cgcgggcggc gttcatgccc cgggtgcgct cggtggagac cagatgcacg
      661 gcccgctggg acaggaatac gccgtcctcg gtgcggccgt tcagggacag catggaggcg
      721 aagcgggtcg cgtcctccag attggtgaac aggcccgaat ggcccgccac gccgtgcagg
      781 aaccgggcgt tgccgtccag cggacagccg ggctgcagta cgccgttttc gtcccggatg
      841 gaggtggggg ccacatcgct gccggagggc aaatagcccg tgcggcccat gtgcagcggg
      901 gcggtcacaa agcgcttagc cgcttcgtcc agcggcatat caaagacccg ttccagcagg
      961 aaacccagca gcaggaagcc catgccggag tcggacacct tgccgcctac cgggctggcc
     1021 agcggatgcc gcagcagcgc gcccagcgcg tccttgtcgc tgcgggcttc ctcctgcagc
     1081 agaaagtggg gcgacagacc tgaggtctgg gtcaacagct gagccacggt gatgttcttc
     1141 ttgtccggcg gcacttccgg cagaaaacga tccaccgtgt catctggccc tagcagtccc
     1201 cgctccacgg cgatcatgca caggggcatg gtcacgaaga cctgcgtcag cgcccctacg
     1261 tcgtaccggg tggagtgcgt cacctgccgc tccccgtccc gcaccagata gccggacacg
     1321 ttggtataca gcactttccc ccgctgcccg gcggcaatgg cgcagccggg aaacgcgttt
     1381 tcacgaatgg cctcttccag caaatggtcg accgcgttcc acataaggcg ttcttccttt
     1441 cattcagttc ttttttgggg gagaaaaaag tccccctgct ccgcaatgta cgtccatcgg
     1501 gcttaggaac ggaaaagccg ccccgggctt gcttccttag cagaaagaaa gaagccctat
     1561 cccgccaccc tgattatacc ggctgcgggg gcggttttca atgttttttt gcgtgaggaa
     1621 gggcaaaaca aaacccggcg gggaaaaccg ccgggtgaag caactggaaa gaacagcggc
     1681 agattccgcc ggtcaggttt ggcgggaacg ctcgatggat ggaggaatcg gttcccacct
     1741 cggcggcacc gccggacagc ccatccccgc cttggcggca cgggcctcca catagcaccc
     1801 gcataaccgg caggttccgt cgaaaagctc ggcgcagtcc cggcagacgt tcagccgccg
     1861 ggagatttct tcgggggcgg cgcgaaactc ctccggcaga gaagcgacat agtctgcgat
     1921 cagctccgcc aattccttgc cctcgggaaa gtcgcccagc agacagcggc agggcggtgt
     1981 catcccagcg tgatctcggc aacggcacag gcgggcagac gaacgctgac ccggctgccc
     2041 gtgagggtca gaccctccag cggccgaacc tccgcgggcg cggaagtgaa atcgttatac
     2101 atatccatgc tgccggacag aatgcggccc cgcacgccgg tggcgtcgaa gtccttcagc
     2161 aggatgtcca gctccatttc gtccgtgggg gaggcgttgg agcaggtgat ggtcagaagc
     2221 ccgtccttca ccgacgcgga ggcgctgagc tggggcaggg tcacgccatt ctcgccgatc
     2281 tgcgcgcact gcacgtccac gtccgcctgt gctgcgtcct gatgggcttt gtacaggtcg
     2341 aaaacgtgat aggtgggcgt tttcaccgta cggtcgccct cggtcagcag catggcctgc
     2401 agtacgttga ccgtctgggc cagattggcc atggacagcc ggtcacagtg gcggatgaaa
     2461 atatccagcg tcagggcggc gacctgcgcg tcccgcagag tgttctgctg gtataggaag
     2521 ccggggttgg tgccgggctc cacctcgaac cacgtgcccc attcgtccac gatcagcccc
     2581 acccggtgct ccgggtcgta gcgatccatc acggccagat gaccctcgat cagcccgtcc
     2641 attttccgag cccgatccag cgtttcaaaa tactgcgcct tggtaaagcc ggtggcaggc
     2701 agcttgtgat cccatccgcc gggaacggta tagtagtgca tggtcaggcc gtccatgaag
     2761 cgcccggcga tggacatgac cttattcgtc cagtccgtgt cgccctcgtt ggggccgcag
     2821 gcgatgcggt acagccggtt gctgccatag ttgcggcagt aggtctgata gcggcggaac
     2881 tcgtcggcgt agaactccgg ccgcatattg ccgccgcagc cccagctttc gttgcccacg
     2941 ccccagtatt tcaccgccca cggctcccgc tggccgttgg cccagcgctc ccgggccacg
     3001 gtggagtcgc cgctgctggt caggtattcc acccactcgc tcatttcctg cacggtgccg
     3061 ctgcccagat tggcgttgat gtagggttcg cagcccagct gacggcacag ctccatgaat
     3121 tcatgggtgc cgaaggtgtt gtcctccacc acgccgcccc agttggtgtt gaccatgcgc
     3181 ttgcggtttt ccttggggcc gacgccgtcc cgccagtgat attcgtcggc gaagcagccg
     3241 ccgggccagc gaagcacggg gatctttacg gcccgcagcg cctccaccgc gtcggcgcgc
     3301 atcccgttga cgttgcggat cttgctgttc tcgcccacgt acaggccgtc atagatacac
     3361 cggcccagat gctccgaaaa atgcccgaaa aggtgccggc gaatggttcc cttggaatgc
     3421 ttggcggata cggtcagttg tgccatgggg actcctttct gcgctgaagc gatcgaaaat
     3481 caatgaaaaa atgatggtac aggcggcgct gccttttgcc gagagaggga cgaagcccgg
     3541 cggctgatga aagcgctaac agccgaaagc ctgtttcgct ttcagcataa cagattttcg
     3601 ctattttgca agaacctttt ttaaccggct ctccgcagaa taaaattttc ttcttcgcgc
     3661 cagcctaccg gcgaggtgat gttgacatga tgccgaagga agaactgctg cagtatatcc
     3721 acaaaaccgc tgacatgggc gtttctgggc tggaggatat gctgcccaaa acggaagatc
     3781 aggagctttt ttccacgctg gagaagcaga agacccagta tgaaaaagtc cgtcaggagg
     3841 cccggcggct gctggagcgg cagggcgaaa gcgttaacga cgcgtcggcc atggccaagc
     3901 tgtccgccaa tatgatgagc gcgggcaagc tgctgtttga tgccagcccg gaaaaaatcg
     3961 cggagatgac cattcagggc accaccatgg gcgtgaccaa gaccatccag caccttcggg
     4021 actacgacgg ggaagacgaa agcgccctcc gtctgggaga gcgccttctg gaaattcagg
     4081 aagaaaatgt agaggaaatg aagcggttcc tgtagcttac cgccacctgt ccttggaaag
     4141 gaagaacagc gccagcgccc ccgggcctac gtgcgcgccg gtgacaggct cgtacttcac
     4201 cgtcaggaac tgggcgttcg ggcaggattc ccggatcagc ccttccagcg tttccgcgtc
     4261 ctctttacag tccgcgtggg caatgccgat cagctgcttt tcgttggggt cgatcttctt
     4321 ccgcagcgcg tccgccagat agcggacggc cttttttcgg ccgcgcacct tgctcgttac
     4381 gatgatgcgg cccagttcgt ccgattccag aatgggcttg atctgcagca cggagcctac
     4441 catggccgcc acgcggctga cccggccgcc ccgctgcagg tgcatcaggt cgtccacggt
     4501 gaacacctgc tgcgtgcggc tgcggtgatt gcgcagcatg gccagcactt cgtccatgga
     4561 cgcgcccgcc ttgcgcatcc gggccgcctc cagcaccagc aggccctcgc ccagcgacgc
     4621 gccccgggta tcgaaggtct cgatgcggac gttggggttt tcctcccgca cccgctttga
     4681 cgccgattcc gacgcggcat acgcgccgct gatgccggag gacataccga tgtacagcac
     4741 gtcgtcgcca ttcgccgcgc ggcgggtcat ggcttcataa taagcctcct gcgccaccat
     4801 ggaggtggtg gcccggacgc cggagcgcag ttttccataa tattctttgt cgtcaaaatt
     4861 ttcggtatcc atgcaggtat attctttgcc gtccagaata taggcaaagg gcatccgctc
     4921 cagctcccat tcctcacaaa ccggcgtggg caggttggcg gatgtgtccg tcaggataaa
     4981 ataactcatg tgcgtgcgca cctcccataa gaagtctgct ccatcatagc cccgaaggag
     5041 gggtagagca aggaaagaac cggggagggg cactctccga aaagtggagc tgcaaaccct
     5101 tgatttttct ggcactctac aaattgtaga gtgccttttt tgaaactcta ccgcccgttt
     5161 tcatatcgta gagcgatcct acaggatcgt aggttgcaca aggctccggc ggcgtgctac
     5221 catgagctcg caacaagccc gatggcgggc agtgaaaagg agtgaccctt ctcatggcag
     5281 actacattct cagctgctgc tccacggcag acctgtccaa ggagcatttc gattcccggg
     5341 agattcacta tatctgcttc cacttcatgc tggacggcgt ggactacccc gacgatctgg
     5401 gcgtatccgt cccgtttgac gagttctacc gccgcatggc ggaaggttcc gacacccgca
     5461 ccagtcaggt gaacgtgtcc gaatatgtgg agtacttcac ccccttcctg gaggcgggca
     5521 aggacgtgct gcacgtcagc ctgtccggcg gcctcagcgg cacggtgaac gccgcccgca
     5581 acgccgccag catcctcggc gagcgcttcc cggagcggaa gatctacgtg gtggattctc
     5641 tgggtgcgtc ctccggctac ggccttctga tggacaaagc cgccgacctg cgggacgacg
     5701 gcatgagcat cgaagcgctg cgggactggc tggaagccca caagctggac gtgcatcact
     5761 ggttcttctc caccgacctg accttctaca tcaagggcgg ccgcgtgtcc aagacggcgg
     5821 gcttcatcgg caccattctg ggcatttgtc ccctgctgaa catggacgac aagggccgtc
     5881 tgatccctcg ggaaaagatc cgcaccaaga agcgggtcat tcgggagatc gtggacaaga
     5941 tggagcagtt tgccgagggc gggctggact acagcggcaa gtgctacatc agcgaatccg
     6001 cctgcatgga ggacgccaaa gccgtcgccg cgctggtgga agcccgtttc ccccatctga
     6061 acggcaaggt ggagatcaac agcatcggca ccaccatcgg cagtcacacc ggccccggca
     6121 ccgttgccct cttcttcttc ggcactcccc ggcaggacta agcaggggcg gtgcccctgc
     6181 accccggac
//
LOCUS       DAWAEA010000037         6185 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2000_length_6185_cov_1.904241, whole genome shotgun sequence.
ACCESSION   DAWAEA010000037 DAWAEA010000000
VERSION     DAWAEA010000037.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 6185)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6185)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6185
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2000_length_6185_cov_1.904241"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1823..1834
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3100..3342
                     /estimated_length=243
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5444..5527
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5797..5806
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1086 a         1831 c         1729 g         1190 t
ORIGIN      
        1 taaacgtatc caccgtcacg gtcactttat cagccagcac ctccgggtcg gtcttgcccc
       61 agcgctgcag catatttaca tagccctgaa tgacggcgat gggcgtgcgc agctcatggg
      121 acgcgtcgct aacgaactgc ttctggctgt tgtagctcaa ttcgatgcgg tccagcattc
      181 cgttgatgac cagcgccaga tccttcagct cgttcttggt gccctccacg ctgatacggt
      241 cgctcaaatt gttggcgctc agggtggcgg cggtctcggc catgtcggtg atgggcttga
      301 gcaccttctg attcagccgt ttccgatggc ggacgaagta gcctacccgc agaagctcga
      361 aggccagcag cgtccacagc agcaccgaca gcacccgcag cggctcaccc agttcccaga
      421 caaagaccgt tccgcccaga atcgtatttt catacagcag cactcgcggg ccctgccgaa
      481 tttccgaaaa gaacaggccg atccggcccg gccagccccg caggtcatcc tccaacggcg
      541 tttccagcac atacactgct gaaaacggtt ggcttgtcaa ctgctcggcg ctgtaagcgc
      601 tcgtttgggc aggctgctct gccaataccc ggtcgatggc ctgattcagc atcggcccct
      661 gggcggcaaa aaacgcgacg ctcaccgcca gcagcatggg cagggtcgtc cgcagcagct
      721 ggccgctggc atacagcgcc acccgcagca ccagtgaaag ccgcaggttg ctcaaaagcc
      781 ccatcacggc gctgtgtacc ttccgggaag cccgattcag cgccgccacg aaacgttccg
      841 agggcttttg ctgccgccgc tgctccttcc gctcctgacg tgcctgtttt tccgtctcct
      901 gctgagcttc cagttcttcg atccgcgtat ggagccgccc cagcagagaa ttttcggtct
      961 ccacggtttc gccgtccaga ataacggcgg tcttgcgctt attcctgctc ataacggaac
     1021 atatacccca cggcgcggat ggtgtggatg gtcttgatgc cgaaacggtc gtcgatcttc
     1081 tggcgcaggt agcgaatgta cacgtccacg atgttggtat cgccggtaaa ctcgtacccc
     1141 catacgttgg tcagcaggtc gtcacgggtg atggcggtgt tctcgtgctg ccacaggtaa
     1201 ttgagcagat cgaactcctt cttggtcagg ttgataggct cgtcgccgta gcccgcgctg
     1261 tagctggcgg gctccatgtg cagctttccg gcgctgcggg ccccggtgct ctgggccgtg
     1321 tccgcccggt gctttttcag cgccacccgg atgcgggcca gcagttcctc gatggcgaag
     1381 ggcttggtca tgtagtcatc cgcgcccatg tccagcccca tgaccttatc gctcacgtcg
     1441 tccttggcgg tgagcatgat gatgggcacg tcgctctgct ggcgcagacg gcggcagacc
     1501 tcgatgccgc tcagttccgg cagcatcagg tccaaaatca aaagatcggg cttccagtcc
     1561 aggcagcgct gcaggccgga gcggccgtca taggcgcact gcacctcgta gccctcgtgt
     1621 tccagttcca gtgtgacgaa gcgtgcgatc ttggcttcgt cctccacgat caatatcttg
     1681 ctcaatcaaa gcgccccttt cccctatggc ctgtccttta ttaccgtatt tccgcctcgt
     1741 ggtagccgtc ctgatcctcc cgcacgtcca cggtggcgcc gtcgggaaac tgcacgttca
     1801 ccggcgtggt gccgctggcg ggnnnnnnnn nnnnacgatt ccgaggcgtt tccgctttcg
     1861 gcgtagcgtc tttttttccc gtttcggcgg attcggtctg cttctccgtc tcctgcgtaa
     1921 agcggctgac cgtctgacgg acgttctttc gaacggtttt cagcatttct tccacggaat
     1981 cggcggaggc gctgcgggtc aggcgaacct tatacccaag cacccacgag aggatcaccc
     2041 cggccagcac cacatagggc gcggccagcg ccgccacgat catcaccagt gcgttcagat
     2101 agacgatgca ggtgtcgccc ttagtgatct tcaaatgcag ccgcaaaagg aaatccacca
     2161 cgccgtgaaa gccgttgtcg gcgggcgcgt tcttcccctc ccccttcagg cggccgttcc
     2221 gttccagatc gaccaacgcc cgggccagac tgccgtcgtg atattccagc aggttcacgg
     2281 cctcctcgta ggtgatgccg ctttttttgc ggaggatctc tatatcttca atggtgtacc
     2341 ttgccacacg ctcgtctcct tccgtccttt ttgacgcatt cagcatacca cattcccgaa
     2401 aaaaatgctt gaagaaatgc tgaaacattt ctgagaatct catgagcggc gcttgacagc
     2461 cttttccatt catggtacgc tttttgagaa attagttgcc tatgcaacca tttagatgca
     2521 ggagaaaaaa gatgccttcc tttacccata gtctgaacat cattggccgc tgcactcaga
     2581 tgcagcgcgg cgccgtcctg aaagaattgg gcgtttgcgg cggtcaggtg ccctatctgc
     2641 tgcggctgtg ccgctgcccc ggcctgacgc aggaagaact ggccaaggcg ctgtatttca
     2701 acaaatccac cgtcacccgg cagatcgccg cgctggagcg gcagggcctt gtgcggcggg
     2761 agccctgccc ggaggatcgc cgctgccagc tggtctaccc cacggagcgg gccttggaga
     2821 tcgtcccgaa ggttcggaag ctggtctgcg gctggaacga tcatctgctg gccgagctgt
     2881 cggaagatga gcgtgaccag ctgcaggccc tgatggagcg ggtctcccag cgggcgcggg
     2941 actacatcga ccgggaagtg gggtgggacg catgaaacgg atcctcggat atctgaagcc
     3001 gctgctgaga cggatgctga cgggctttgg catcaagctg gcgggcacgg tgatggatct
     3061 ggccattccg tggatcttgg cccacatcat tgacgaggtn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntgatggac ttccggctca
     3361 gtctggtact gctggccacg ctgcccctgc tggcgttggg cattgcgctg atctcccgcc
     3421 ggggcctgaa gctgtacgga accgtgcagc agaacgtaga ccgcatggtg cgcaccgtcc
     3481 gagacgacat taccggcatt cgggtcatcc gcgccctgtc caagggcgac tatgaatgcg
     3541 cccatttcca gcgggacagt gaagcccttt cccgcagcca gattcatgcc gactcggtca
     3601 tggcggccac caacccgatg atgcagctat tgctcaatct ggggctgacg gcggtggtgg
     3661 tctgcggcgc ttactgggtc aatctggggc tgaccaagcc cggcagcatc attgcgtttc
     3721 tgacctattt caccatcatt ctcaacgcca tgatgtcgat aaacaaaatg ttcgtcatgc
     3781 tctcccaggc ggcggcttcg gcggatcgta tcgccgaagt gctgaacctg ccggacgagg
     3841 tgccgcaggg cggactggca cggctggaaa gtcgctcccg catcgttttc aaggacgtgt
     3901 gcttttccta tgcccagtct gcccgcgggt acgatttagg ccccgtgacc ctttccgtag
     3961 ggcagggcga aacgctgggc gtgatcgggg ccaccggcag cggcaaaacc acgctgatcc
     4021 agctgttgca gcgattctat ccgctgggca aaggcgctat ctacgtggac ggcaagaaca
     4081 tcgccgggat ggaggaagcc cggcttcacg ggctgttcgg cgtggcgttc cagtcggacg
     4141 cgttctttgc cggcaccatt tatgaaaata tcgatcttgg ccgtgggctg ccccgggaag
     4201 ccattgaaaa ggcggcccgc tgcgcgcagg cggaagaatt tattctgacc acgccggagg
     4261 gctacgagca tcccgttcgg gccaaggcgg tcaacctgtc tggcgggcag aagcagcggc
     4321 tgctcatcgc ccgggcgctg gccgggaacc cggagatcct cattctggac gattcatcca
     4381 gcgcgctgga ctaccgcacc gacgccatga tgcgctccgc cgtccggcag gaatacccga
     4441 acaccacgct gttattggtg gcccagcggg tcagctccgt caaaaatgcc gaccagattc
     4501 tggtgctgga agaaggccgc atggcggggc tgggaacgca cgaagccctg ctgcgggatt
     4561 gctcgctgta cgcccagatc gcgaaaatcc agatgggagg ggacgacgat gccgccgcgt
     4621 aacggagtga ttcaaaaacc gcgcaacgcc aaaggaacgc tgcgccgcct cgggcgctac
     4681 ctgtggcaga gcgcgccgct gctgatcctg gcgcttggcc tgagcgtagc gggaaacctg
     4741 ttccagctca tcggccccac cctgtgcggc aaggccatag acgcggtggc gggcgggccg
     4801 ggaaaggtgg attttgacac ggtgttttcc tatgcccggc taatggtgct gttctacgcc
     4861 gcatcggcgg ggctggggta tctggtgccc cagatcgtgc tgcgcatcag ccagaagact
     4921 gtgcgtatga tgcgggccga tctgttcgcc aagctgatgc gcctgccggt gcagtacttc
     4981 gacacccggc agaccggcga tattttgagc cacatcagct acgacatcga caccgtcaac
     5041 acctcccttt ccaatgacct tgtgcagatt ttgaccagcg tcatcaccgt ggcgggcgcg
     5101 ctgagcatga tgatccgtat ttcgccgcta ttggtgctgg tgtttgcggt cacggtgccg
     5161 ctttccgtgc ttttcacccg gttcatgacc cggcgggtac ggcccctgta tcacgaccgt
     5221 tccaagtgtc tgggcgaaat gaacggcttt gtggaggaat atgtttccgg ccagaagacc
     5281 atccgggcct atcaccggga ggcggacgtg ctgaatcgtt tcgatgaaaa gaacgaagcc
     5341 gccgtggaaa gctactaccg ggcggagtac tacgccagca ttaccggccc gtcggtgaat
     5401 ttcatcaaca acctttccct gacgcttatc agccttttcg gcgnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnagc tgatggacga gccggaggag cccgccgacc tgccgggggc gaagccgctg
     5581 gacgtgcggg agggccaagt ggacatcgag cacctgaact tcggctacga ccctcaccgg
     5641 ctcattctgc gggatgtgaa cctgaccgcc gctccgggca gcatgatcgc catcgtcggc
     5701 cccaccggcg cgggcaagac tacgctggtg aacctgctca tgcgcttcta tgacccccag
     5761 tccggggcga tcgccatcga cggccagtcc atccacnnnn nnnnnngtgc tgcaggatac
     5821 atggctgttc aacgccacgg ttcacgacaa catcgcctac gggaaccccg gcgcaacccg
     5881 ggaaaaggtg atcgaagcgg ccaaggccgc ccacattcac agcatgatcc agcggctgcc
     5941 caagggctac gacaccctgc tgaccgaaga cggcgtgaac atttccaagg ggcagaagca
     6001 gctgatgacc attgcccggg ccatgctgca gcccgcccac ctgctgatcc tggacgaagc
     6061 cacctccaac gtggacatcc gcaccgaagc cctcattcag gaggccatgc gggcgctgat
     6121 gcaggataag acctgcttcg tcatcgccca ccggctgtcc accatccgca gcgccgacct
     6181 gatcc
//
LOCUS       DAWAEA010000038         6120 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2022_length_6120_cov_1.950866, whole genome shotgun sequence.
ACCESSION   DAWAEA010000038 DAWAEA010000000
VERSION     DAWAEA010000038.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6120)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2022_length_6120_cov_1.950866"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    302..311
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1900..1938
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3833..3842
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5384..5488
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1238 a         1814 c         1829 g         1075 t
ORIGIN      
        1 atcctcaatt tcttcctgat ggggtggacc attcagcggg ccgtggggct ggacgaaaag
       61 gccgccgccg ccaagctgaa gctgtcgcag atgctgcgga tgctgatgct gatcgtcatc
      121 tgctgcgtag gtatcgcgct gaactgcttc cagacggtag cggtgatcgt gcccctgttc
      181 ttcccgcgta tcgcggtggc cttccgtcca ctgtttgaca aaaagaacgg tcaggccacc
      241 tccgggggga aggacgatgt gaagatcgat taaacgcaga agggaaacct tccgcgttga
      301 tnnnnnnnnn nggcagaagc gcggcgaaag ccggccgctt ctgctcatca ttaccaaagc
      361 aaaggaggga tgatccgaaa tgaatcgaaa atccgcgcgg tttcgtgcgg tggacgttct
      421 gctgatcgcc atgatgatcc tgccgctggt agcggggatg gcgctcaaga tcctgacgac
      481 tccagcgtcg gaagggatcg agatcagcgg ggcgcgcatc tactttacca ttcccatgcc
      541 ggtgcaggat ctgcccatta ccgaaagtca ggtgaactcc tggctggtga tgatctccat
      601 cctggggctg tgcctgtatc tggtccacgg catcaccgtg aagccgggca gcaggcgcca
      661 gctggtggcg gaatggatcg tagaaaagac caacggtctg gttcgggaaa acatgggcaa
      721 gcgcttcatg ggcttcgcgc cctttgtggc gggcatcatg gccctgtccc cgttttccag
      781 tctgatgagc tggtcggcct ccggccccct acttccgaca tgaacgtcgt ggccggatgg
      841 gccattctgg tgttcgtgct cattacccac tacaagctcc agggaggcct tgggccatat
      901 gtgaaggggt tcttcgagcc ggtgccggtc tttgcgccct ttaacatcat cggcgaattc
      961 gccacccccg tatccatgtc gttccgtcat tacggaaacg tgctctccgg cgtggtcatc
     1021 tccacgctgg tggcctatgc gctgcagggc cttagcaagc tgctgctggg ctggctgccc
     1081 ggcatcctgg gaagcttccc cctgctgcag gtcggccttc cggccattct atcgctgtac
     1141 ttcgatattt tcagcggcct gatgcaggcg ttcattttcg ccatgctgac catgctgtat
     1201 atcggtacgg ctgcccccga ggaggaataa gcaggcggcc ctgccaaaag caacgcaggg
     1261 aagcccattt gtccacacac gctgcaaaca aggaggattc tatttatgga actcgctatt
     1321 ggtatcattc tcgccggctg cgccatcggc gcgggtctcg ctctgatcgc cggtatcggc
     1381 cccggtattg gtgaaggcaa cgccgttgcc aaggcctgtg aagccatcgg ccgtcagccc
     1441 gagtgcaaga gcgccgttac ttccaccatg atcatgggct gcgccatcgc ggaaaccacc
     1501 ggtctgtacg gcctggtcat cggcatcctg ctgatcttcg tggcccccaa cctgtttgtg
     1561 aatattctcc tgaaggccat tggctgacgg atccctgaat tggaaaggca atagtatatg
     1621 agcgtacagt cattggacat catttcggtg aacctgtggc agatcctcat ttcccttgcc
     1681 aacctgctga ttatctacct gatcctgaaa aaagtgcttt tcgccaaggt gcagggcgtg
     1741 atgaacaccc gccagagtca ggtaacggaa atgtacgacg cggcggacaa gagccgcacc
     1801 gacgccgaaa gcatgaagca ggaatatgaa gaaaagctgg ccggggcccg ggacgaggcg
     1861 gacaaaatcg tccgcaatgc tacccagacg gcccagcgcn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnntg cttacggacg tgcggggaga gatctccgac ttggccgtca
     1981 gcatcgcatc caaggtcgtg gaacgcgaga tccgcaagga agaccaggat acctttgtgg
     2041 atgaatttat caggaacgtg ggtgaccggc agtgacggag cagagcagag agtacggcga
     2101 aggcctgtac gccctgtgcg cggaggaagc gctgtccggg gacgtgctgg agcagctgga
     2161 cgtgctgaac ggcagcttca aggctcagcc cgattacctg cggcttttgt ccaacatgtc
     2221 tctggcccgg gaggagcggc tggatattct ggacaggacc ctgcggggac aggtacaccc
     2281 ctatgtgctg aatttttgca agatccttct ggaaaagggg cttttgcatg aattctccga
     2341 ctgcgtgaag gcctaccggg cccgttatct tcaggataac ggcattgtgg aggccagcgt
     2401 caccaccgcc gtcccgctgg acgacgggca gcggaaaaag ctcatggaaa aactgagcgc
     2461 catgaccggc aaacagattg ttttgaagga acggcttgac ccggcggtgc tgggcggcgt
     2521 tcggctggag atggacggca agcgctacga taataccgtc cagagccgcc ttacggccat
     2581 ccatcgggcc atcggcggtc aggcctgata acaggcggtg agacaatgca gttaaaaccc
     2641 gaggaaattt ccaagctcat caaagagcag atcaagcatt acgaaaataa gatttcacag
     2701 gcggataccg gcaccgtcat catgatcggc gacggcatcg cccgggcctc cgggctggac
     2761 aagtgcatgg ccaacgagct gctgcgcttc tccaacggcg agtacggcat ggcgctgaac
     2821 ctggaggaaa actccgtggc catcgtcatg ctgggcagcg acgagggcat ccgcgagggc
     2881 gacgtggtgg agcgtaccgg caccgtggtg tccgtgcccg tgggcgaaaa gctcatcggc
     2941 cgcgtggtca acgcgctggg ccagcccatc gacggcaagg gccccgtgga aaccagccag
     3001 tactatccca tcgaacgcaa cgcccccggc atcatcgagc gcaagggcgt gtccgttccg
     3061 ctgcaaaccg gcatcaaggc catcgacagc atgatcccca tcggccgggg ccagcgcgaa
     3121 ctcatcatcg gcgaccgtca gaccggtaag accgttatcg cgctggatac catcctgaac
     3181 cagaagggca aggatgtgat ttgcatttat gtggccatcg gccagaaatg ctccaccgtg
     3241 gcccagatgg tggataccct gaccgcgggc ggcgctatgg attacaccat tgtcgtgtcc
     3301 gcgacggcca gcgaacagtc ccccctgcag tacatcgcgc cctattccgg ctgcgccatg
     3361 gcggaatact tcatggatca gggcaaggat gtgctgatcg tctacgacga cctgagcaag
     3421 cacgcggtgg cctaccgtgc cctgtcgctg ctgatccgcc gtcccccggg acgcgaggcg
     3481 taccccggcg acgtgttcta cctgcacagc cgccttctgg aacgggctgc ccgggtggac
     3541 aaggcctacg gcggcggctc cattacggcg ctgcccatca tcgagaccca ggcgggcgac
     3601 gtttccgcct acattcccac caacgtgatc tccatcaccg acggccagat ctttctggag
     3661 accgagctgt tccactccgg cgttatgccc gccgtcaacc cgggcatttc cgtcagccgc
     3721 gtgggcggca acgcccagat caaggccatg aagaaggtgg ccggcagtct gaagctgctc
     3781 tacagccagt accgcgagct gcagggcttc gcccagttcg gctccgatct ggnnnnnnnn
     3841 nncacccggg ctcgtctggc gcagggcgag cgcattgtgg aagtgctcaa gcagaagcgg
     3901 aaccgtcccg tggccgtgga aaatcaggtg tgcattttct acgccgtgac tcacgatttc
     3961 ctcaaggaag tcaaggtcag cgacgtggcc gagtatgagg ccgggctgta cgagcgcatg
     4021 gaggcccagc attccgacgt gctgaacgcc attcgggaaa gcggccagct gagcaaggaa
     4081 accgaggagg ctctccggca ggggctggaa agctacaccc gcgacttcct cagcgcccgg
     4141 aaggccggct aagggaaagg aggctgcaac atggccggag cgtccatgaa agacatcaag
     4201 ctgcgcatca agagcgtgga aagcaccatg cagatcacca aggccatgca gcttgtggca
     4261 acctccaagc ttcgccgcgc caaggagcgg atggaagcca gcaaacccta tggtcagatc
     4321 tccggcgcag ccatggccgc ggccgtggcc cgctgcaccg accccaacgt gcccttcgtc
     4381 accccccgcc cggtgaagcg ccgctgctat gtggtcatcg ccggggaacg gggactggca
     4441 gggggctaca acaacaacat tttcaaggcg gtggccgccc acagcgaaga aaccgcctac
     4501 tgcgtgctgc cgctgggccg caaggccatg gagaaatacg cccatctggg cgtggagctt
     4561 ctcagcgacc agtatgcccg ggtggaaagc atttccgtag gttcctgcta cggactggcc
     4621 aagcggctca tcgagggcta cacaaacgag gagttcgatg agatctggct ggcgtacacc
     4681 tccttccagt ccatgatgac gcaggaggtt cagctggagc agctgctgcc catccgcaag
     4741 ccggaaacct cggaacctct gccggtatcc gccgtgtacg agctggaacc cgccgacacc
     4801 ctgaaggcgg tcatgccgga ctttttggcc ggacggctgt acagcgccct gtgcgattcc
     4861 tttgccagcg aggtagcgtc ccggcgcaac gccatggatt ccgctaccaa gaacgcgggc
     4921 gaggtgatcg acggcctcag gctccgcttc aaccgcgccc gtcagggcag cattacgcag
     4981 gaaatcaccg aaatcgtggc cggtgcggaa tcgtaacggc atcgcgaaag gagaacacct
     5041 attgtgagca gtcaaaacat tggcaaagtg gtgcagatca tcgggcctgt gctggacatc
     5101 cgcttcgagg acggctgcct gcccgaactg ctcaacgcca ttgagattca gaacggcgac
     5161 aagaaaatcg tcgccgaggt ggcgcagctg atcggcgaca acgtggcccg ctgcatctcc
     5221 atgaacgcca ccgacggcat ggtgcgcggc atggacgcgg tggacaccgg aagccccatc
     5281 accgtgcccg tgggcaacga atgtctgggc cgcatcttca acctgctggg gcaggccatc
     5341 gacaatcagc ccgacccgga agccaaggaa cgctggccta tccnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnngc ccccagctat gaggagcagg aaacctccac
     5521 tgaaattctg gaaaccggca tcaaggtggt cgacctgatc gccccctacg ccaagggcgg
     5581 caagatcggc ctgttcggcg gcgcaggcgt gggcaagacc gtgctgatca tggagctgat
     5641 caacaacatc gccaagcagc acggcggtct gagcgtcttc gccggcgtag gcgagcgcac
     5701 ccgtgagggc aacgacctgt acaacgaaat gaaggaaagc ggcgtgcttt ccaagaccgc
     5761 gctggtctac ggccagatga acgagccgcc gggagcccgt atgcgggtag ccctgtccgg
     5821 cctgaccatg gctaccagcc cacgctggcc accgagatgg gcgcgctgca ggagcgcatc
     5881 acctccacca agaagggctc catcacgtcg gtgcaggccg tttacgtgcc tgcggacgac
     5941 ctgaccgacc cggctcccgc cactaccttc gcccatctgg acgccaccac ggttctgagc
     6001 cgtgccattt cctcgctggg catctacccc gccgtggatc cgctggactc caccagccgc
     6061 atcctgaccc ccgagatcgt ggggcaggag cactacgacg tggcccggca ggtacaggga
//
LOCUS       DAWAEA010000039         6005 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2079_length_6005_cov_2.396471, whole genome shotgun sequence.
ACCESSION   DAWAEA010000039 DAWAEA010000000
VERSION     DAWAEA010000039.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 6005)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6005)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6005
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2079_length_6005_cov_2.396471"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1086 a         1859 c         1910 g         1150 t
ORIGIN      
        1 tcgctcttgt agcgcttgag gacaaagtgg gtggccgtgc cggtgacggt ctccagcgtg
       61 ctcagcttgg tggagacgaa gaaggccagc tccttgaggg tgtgggcctc caccagcagc
      121 agcaggtcgt aggcgccgct catcaggtac acggacttga cctcgtcaaa gcggtagatg
      181 cgctccgcta tcgcgtcgaa gccctgatcc cgctggggcg tgacccgcac ctcgatcatg
      241 gcctccaccc gttcacggtc ggtgcggtcc cagttgataa tgggcgcgta gcgcaggatc
      301 acgccgcttt tttccatctg atcgatggtc tgagctactt cttccagcgg cgcaccggtc
      361 atggtggcca gctgttccag cggcacacgg cagtcttcct tcaggatttg caaaagctcc
      421 atttgcagac tttgcatttg ggttcctcct tgcttaaaat aggccaacga tctggccgtt
      481 gtcgtctacg tcgatcttca aggctgcggg agccttgggc aggccgggca tggcaatgat
      541 gctgcccgcg aaagcgacta cgaagcccgc cccggcgctg agccggacgg agcgaatggt
      601 gagctcgtag ccctccggcg ctcccagttt ggcggggtcg tcgctcatgg aatactgggt
      661 cttggcaatg cataccgggc aagagccgta cccctctttt tccagcgctg caaggccttt
      721 cagcgcctca gcggagaaat tcaccgaacc ggcgtgatag atgcgccggg ccacggcttc
      781 gatcttctcc cgcaggggaa gctcgtccga gtaggtgaag gaaggcttgg ggcagggatt
      841 ttcgtccgcc gtgcggcaga ccagctccgc cagttccatg gttccgctgc cgccgtgcgc
      901 ccagccgtcg cagggggcac agggcacgtt ctgttcgacc agccgttccc gaaccagctg
      961 aagctcctcc tccgaatcac cggagaagcg gttcagcgcc accaccaccg gcgtttgcca
     1021 tacgtggcgg atgttggaaa tatgactttc cagattggca agcccctgcc gcagggcttc
     1081 ggcgttcggt tcgctgtagt gtcctttttc cacgccgccg tggtgcttca gcgcccgcag
     1141 cgtggccacc agcacgatca cgttgggcca caggccgttc atgcggcact tgatgtccag
     1201 aaatttttcc gcccccagat cggctccgaa acctgcttcg gtgacggtgt agtcgctcag
     1261 cgccatagcg gttttcgtgg cgacgacgct gttgcagccg tgggcgatat tggcgaaggg
     1321 gccgccgtgc atcaggcagg gcgtgtgctc catggtctgc accagattgg ggcagagagc
     1381 gtcccgcagg agcgccgtca tggcccctgc cgcgcccacc tgcgcggcgg tcacaggctc
     1441 gccgccgaag gtatgggcca ctacgatgcg gcccagccgg cgcttcaggt ccttcaaatc
     1501 agccgccaga cagaagaccg ccatgatctc gctggccgcg gtgatgtcga agccgtcctc
     1561 ccggggggtg ccgttgatcg gcccgcccag gccctgcacg atctggcgca gctggcggtc
     1621 gttcacgtcc agacagtgcc ggaaacaaat tttccgcggg tcgatgccca gggcattgcc
     1681 ctgctgcacg tgattatcca ccatggcgca cagcaggtta ttggctgcgg tgatggcgtg
     1741 caggtcgccg gtaaaatgca ggttgatatt ctccatgggc accacctgcg catagccgcc
     1801 gccggtcgcg ccgcccttca tgccgaacac cggccccagc gaaggctccc gcagggccag
     1861 catggcgttt ttgcccagtt tgcgcattcc gtccgccagc gccacggaca ccgtggtctt
     1921 tccttccccg gcgggggtgg ggttgatggc cgtcaccagc accagccggg cgcgggcgtc
     1981 ccgctggggc agcgccgccg ggcccacctt ggcgatatac cttccatagg gggagagggc
     2041 ctcggttgcg atgcctgctg cctgtgcgat ctcctcaata ggtttcattt ctgcggtgcg
     2101 ggcgatttcc aaatctgtcg ccactgtgtc ccctcctttc gcccataggg cgtataaaga
     2161 tacgggattc agtataacgc aaatggagaa tggatgcaag cgcaagcatt gacgcagccc
     2221 gccccatccg ttataatgaa aggtacgctc cgcgcaacgc tatttcggaa aggagagaag
     2281 caggacgaaa gccatcgtac ttcatccgtc caaaggaaag attcagggca tgatcctcgg
     2341 cgcatggctg acttctctgc tttgtctgct ccttccgccg ttcagcgtgg cggcggtgtt
     2401 cataatgccc ctgtttctct gcccactggc aggctccaaa aaagaggcgt gggcctaccc
     2461 ggtggggctg gcggcgatcc tgctgggcgc gtcgctgtgg acgcggcagg ttttcttccc
     2521 ggtgtggctg ctgctggcta tcgcgcctcc gctggtgctg gcgtaccgtt ttccccacat
     2581 gaagctggaa tttcggcagt ttctgttctg gtttgtgggg gcctatgctt tgagcgccgt
     2641 tatggtgctg gcgtggccgg tgatggccgg ccttggctcc ccggcggaat ggatcagcgc
     2701 ccggctggtg gaactgctca aacaatccaa cagcggcaac gcgctgctgt accggctgac
     2761 ggcggcgggg tatctttccc tgccggagga aagcagcgcg ccgtcactgc tcagttccct
     2821 gctggatcag cggcccatga gccgccagct gtatctttcg ctggagcttc gtttccgcca
     2881 gagcctgacc gcgctgctgc cctccctgtg gatgcaggtg atcctgctgg gtgggctgat
     2941 gaccggcctt cgggtgtgga agacccgctg cacctttctt ctggtggacg aggagaaaaa
     3001 gaccatcccc acgatggcga tctctcccgg cttttccggg ctgtccttcg gccgggggct
     3061 ttgcctgctg tttctgggcg ctggcgctgc ggggctgctg atctccatca gcaccatcaa
     3121 tgacgtggcg ctgcagctgg cccgggtgct ggtcagcttt tgttccatcg gcttccgtct
     3181 cagcggcgca atgacgctct gcggcctcat catgcggcag gacgaggacc ggcggctgtg
     3241 ggcgggtctg ctgaccggcg cggtatgggg gctggccccg gcgatcctgt ttctgttagg
     3301 cattacggat cccatgctcc attatcggag ccggggatcc tttcaaaagc aaaaggagga
     3361 tgaatagatc atgaaggtta ttctgttgtg cgatgtaaag ggaaccggga aaaaggacga
     3421 tattctcacc gtgtccgacg gctttgcccg caactatctc ttcccccgca agtgggcggc
     3481 ggcggccacc cccggcgcgg tgaaggagat cgagcgcaag cgcgccaacg aggctcgtct
     3541 ggaggcggag cgccgggccg aagcccagaa gaccgccgat ttcctcaagg gtaagaccat
     3601 caatctgcag gtgaagtgcg gtgataaggg ccgtctgtac ggcagcatca ccaatcagga
     3661 gatcgtggac gccctcaagg cccagcacgg caaggagatc gacaagcgca agctggaatg
     3721 cgacgcggtg cgccagaccg gcgactacga cgtgaccgtc accgtgtaca acggcatcaa
     3781 ggctgccatg aagctccacg tggagccgat ggcgaagtaa aggaacacgc ggcgcaccgt
     3841 tcgcggacgc ctcataccgg caaaaaaatc atgcaccagc aggaaaggag cgaacgcccc
     3901 gatggaaagt caggattata ttccggcgct gccgcccagc agcgtagacg cggagcgctc
     3961 cgtgctgggc gcggtgctgc aggacagcgg tgcggccacg cttgcctttg agaccctgtt
     4021 gccggaggat ttttacaccg ccgagcaccg ggaaattttc tccgccatgc agacgctgca
     4081 tatcgcgggc aatcccatcg atctgatgac ggtggggaac gagctttccc gccggggcac
     4141 gctggacagc gtgggcgggc ccgcctatct gctggaagcg gtgcgcttcg tgcccaccac
     4201 ggccaacacc cgcacctata tcgaaatcgt gcaggaaaag gccacgctgc gcaagctgat
     4261 cgccgcgtcc cagaccatca gccgccagtg ctatgcccag accgaccctg tgcccgacgt
     4321 gcttcgggat gcggaacggc tgatcttcga tatcgtcatg cgtcggggcg gcgcggaaac
     4381 gctggaaccc attcagaagg tgctgctttc caccttcgac cgcattgagg aattgagcct
     4441 gctgaaaggc aagctggcgg gtgtgcctac cgggttgtac gatctggatc ggatgctcac
     4501 cggccttcac ggcggcgagc tggtgctggt gggggcgcgc cccgccatgg gtaaaacctc
     4561 catggcgctg ggcgtgacgc tgttcgccgc ccagaaggca cacaaatgcg tggctttttt
     4621 cagtctggaa atgcctgcgg agcagatcgg cctgcgcctt ctgtgctccg cggccaacat
     4681 caacatgcag cgcgtccgca gtgggatgct gtccgatgag gaatgggtca agctgggcga
     4741 cagcattaac gccctgagca actgccgcat ctacatcgac gatacttccg gcctgacgcc
     4801 ctcccagctg cgttcccgct gccgccgtct gatgatggag caggggctcg atctggtagt
     4861 gctggactat ctgggcctga tgggcaccga caagcgggtg gagaaccgtc agctggaagt
     4921 cagcgagatc agccgccagc tcaagtccat tgcgctggaa ctgaagattc ccgtgctggc
     4981 ctgcgctcag ctgagccgcg ccaacgcctc ccgcgaaaac aagcgcccca tgctcagcga
     5041 cctgcgtgat tcgggcagca tcgagcagga cgcggacgtg gtcatgttcc tgcaccggga
     5101 aggctactac gccgccgcgg gcgggccgga ggacggcccc aagcccgacg acaaccaggg
     5161 cgaagtcatc gtcgccaaac agagaaacgg ccctatcggc accgtcaacg tggagtggca
     5221 ggcggaattt gcccgctaca ccaatctgcc cggcactcgc gtagccgtat acgcggacta
     5281 aatctgtcag aaatcagggg gagaacatgg cacagctacc cattgccgag cttggcaagg
     5341 atcaaaagtt tgaaggcttt ctgctggttc gttcggcgga tcagcgcacc ggcggcaacg
     5401 gcgcaaaata tctggatatg aacctgaccg accgcaccgg cgaggtcaac gccaaggtct
     5461 gggatggcaa caccccctgt cccgcgcccg gctccgtcat caaggtacgg ggcacgacgc
     5521 tggaatacaa cggtcgcctg cagctgcggg tggagcgtat ccgcaccatg gcggagggcg
     5581 atcccgtcga tctgtccctg ctggctccct gcgccccgga agccccggag gccatgctgc
     5641 aggagcttta tgacacggcg gacgctttta ccagcgaacc tttgcggctc atcacccggg
     5701 aaatgctgga tcgtttccgg gaaaagctgc tgtattatcc cgccgcccag cggatccacc
     5761 acgccgagcg gggcggactt ctgcatcaca ccacggggat gctgcggctg gccaaggcca
     5821 tcctgaccca gtacacgtgg ctgaacgccg acctgctgct ggcgggcgtg atcctgcatg
     5881 acctgtgcaa gacggaggaa atggactcca acgacatggg cgtggtgcgg gactacagcc
     5941 gggagggtct gctgctgggc catctggtgc tgggcgtgaa ccgcattcag gaggcggcca
     6001 acaag
//
LOCUS       DAWAEA010000040         5817 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2173_length_5817_cov_2.033322, whole genome shotgun sequence.
ACCESSION   DAWAEA010000040 DAWAEA010000000
VERSION     DAWAEA010000040.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 5817)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5817)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5817
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2173_length_5817_cov_2.033322"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5450..5459
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1202 a         1865 c         1710 g         1030 t
ORIGIN      
        1 gtgatcggca aggaacacct gcgaggactg gaggacggca cgatggtgct ggtgtgcaac
       61 cacggcgagc tgtacggccc ggtggtggcg aatctgtaca ttcccgtcac cttccgcccg
      121 tggaccatcg cggaaatgat gaaccgggac gtgatcatcg agcacatgta tcagggcacc
      181 atgatgcggc agaaatggct gcccgaaagc tggaagcggc ctatcctgcg cataatcacg
      241 ccgctgcttc tgtgggtgtt cgacagtctg gaagccattc ccgtctaccg aaacgacccc
      301 cgcaagctga tcggcacctt ccgggagacc atcgacgcga tgcaggccgg ggacaacatc
      361 cttcttttcc cggaaaacga cacaggtcac gccgtgggcg aacggggcta cgcatcggaa
      421 ggcgtgggca ggctgttcac cggctttgcc atgatcgcgc cgctgtatta tgccaaaaca
      481 aagaaaaaag cggttttcat tcccgtgtat gcctccaaaa agacccgcac cgtccgcgta
      541 gggcagggga tcgaatacca gccggacaac ggggccaacg acgaaaaaat gcgcatcgtc
      601 gacgggcttc tttcccagat gcaggccatg tacgaggagg agaaatagcc ccgccttcct
      661 ttgacttttc ggccctccaa agcccgacat tcccgttttg ccttgacgga taagcccatt
      721 caggatacaa tagccttgct tttcatccgg cggccgtatc ctgaagggct tcttttatac
      781 cgctatgatc ttttcttccg agggaaacgg cgcggacaag gcaaaaccag gcgacagaaa
      841 ggaagaatgc ccatgtttgg acgccgcccc gaaggaaagc gggtcaagca tctcgacccc
      901 atcgttcaga ttacccccta cctgatgcca tggtgctgcg acgcgcaggt ctttttggcc
      961 tacgacgcgg aatacgagcc gctgctgcgc tacatgagcc acaagagccg ggagggcgtg
     1021 aagatcacct ttatggagct gatcatcgcc gcctatgtcc gggccatctc tcaggtgccg
     1081 gaggccaacc ggttcatcat gaaccgcaaa ttctacaacc gtaccgagct ggcggtgagc
     1141 ttcgtcgtcc tgcggcccac ggaagacggg cagcccgagg aaaacgccgt caaggtgaaa
     1201 ttcgacccca gcgacaccat cttcgaggtc agcgcccgcg tgaagcagac cattgaagaa
     1261 ggccgccgga cggacgatga cagcgccgcc atcaaattgg cctccgccct gctgaaggtg
     1321 ccccttctgc ctaatctggt ggtgcatgtc gccaattttc tggatcacca cgatctgttc
     1381 cccaaggcgc tgatggacgc gctgcccttc cataccagcc tgtttgtaac caacatggcc
     1441 tccatcggca tgacgcgggt gtatcatcat atctacaatt tcggcgatac caccatgttt
     1501 ctgtcgctgg gctgcccaga gcgttcccac gcggtggacg ctaccggcaa ggtgttccgc
     1561 aaatgcaccc tgcccatggg catgaccgtg gacgagcgca tctgcggcgg cgcggtgtac
     1621 gcccggctgt ttgcgtccat gaagcactat ctgaaacacc cggaggagct ggaagcgccc
     1681 ccggacaggg tgtattacaa cgagggcgcg gaatatcatg tgcccaagcc ggacaacgta
     1741 gcgctgcccc attccgtcac tctgaacgca tgatcaaaat ttccgccgtg ggatagcgct
     1801 ggcgaatgtc ccatttttcc cactcttcat tataagcaaa gcaccgggcg ctggccccgc
     1861 caaagcccag cgcgtcgcag tggaacccct gcaggggctc cactgctttt tgtatgcgtt
     1921 cgtcgctgag ggctccgtcc atgcgggtgc gcctgccaag gcaaatgcgc agctgggtgc
     1981 ggtcgccgtc ccagcccacc tccagccggg cggctttggc cagccgcagc acggtctggg
     2041 tggtctccgc atccagaagg ccgctgaccc ggtcctcccc aatggcggcg ctgccggtat
     2101 attccaccgg attgctgccg gtgcggggca tccgcccggc cagcgccccg ggcacggaga
     2161 aggcctcgtc ctcgatattg ccccgggcaa aaaccgtctg actttcctgc gtctgctgtt
     2221 cttcgtcgcc ttcctgcggc tgggcttctt cctgcagggc cggattgacg gcgcacaccg
     2281 caatgatggg atccttcatt tccgtatcca gatcccgcag aaggcaggtc accgtatgcc
     2341 gaggcgtcag cttggctttt tccagccggt ccataaaaat atccagatag gtgctcagcc
     2401 gcatcccgaa atccggctta atggcgtgca gcacctcctc gccccggccc agcgccacgg
     2461 cgaccatggc gttgggccgc atggtggaga tggtggtgat cttgtgcaga agcggctgca
     2521 gccgctcccg ggaagccagc tcgtagccga agacgcacaa tttcagctgg cagaaattca
     2581 cggtgcaggg cgtggtggcg gtcagcacgg ccatcgcgtc ctcaaagctg gctcccgtgg
     2641 cggtgaaaac cgagtattcc ggcgtggcgg aagtatcgtc gccgctgccg ttcaccgggg
     2701 tctgaacgct tagggtcagc ccgccgttct ccgccgcgtc cacggcaaaa cacagggcgt
     2761 aggtttgccg ctccatcacg gagatcacgt cgcagcctgt aaggcagccc aggcaaaggc
     2821 ccagcgtcca gaaaatcagc cagaactttt tcatgctttc tcctttcggg ggcggcacag
     2881 acaaagggcg caggccgcgg ccagcgacag cccgtaccgg gctggcagga tccacgggat
     2941 cccgtccagt atggcggagg gtgaaaacgc cagcaggatc agtccgggca gcagcagagc
     3001 aggcggccac aggcccttgg ggatcttcgg aaaagcccgc gccaaaagca gcttgcccgc
     3061 cgtggtcgtc cccagcaggg aaacgggcag catcagcatc cagaacagaa tgcccagccc
     3121 gtagatcaga atattggagc cgtattggtt cagccccgcc agtttgcggc ctgcgtccca
     3181 gctttcctcg ggctgccacg gttccagcat gctcagccac agcgcccaga ggacgcagca
     3241 caggagcgga acggcaatga acagccagtc cttccgtttt cctctgctgc cgggctccgg
     3301 gcttccgcgc ctgagaggga acagctccag cagcactacg ccccatacgg ctcccatgcc
     3361 cggcacagcg gccagcagcg ttttgccgat cccgtccccc agcaggggat gcagccgttc
     3421 cagactcaca tcgccgccgg agaagaccgt ggagcatagc accagcgccg ccagcagcca
     3481 gcgcagcgta tacgccccgt aggccgcgcc cttgtcccgg ctcagccagc tggataccac
     3541 cgggaaaaac gtcaccacca gcggccatgc cagcggcgtt ttcgccggaa tcagatcgga
     3601 aatcagcgaa ttggaggcca gcatacacag gtatgcgtcc atgcacaggt tagggatcaa
     3661 cagcagcgcc cagtaacgcc ggccgccttc cagcccgccc cggcaggccc gccgccaaag
     3721 gccccataac agcagtgtgg ggacggccag aaaaacagcc gcctgccagg tggtctgctg
     3781 cagatggtca tagccgaact ggatcgccca taaaagcagc tgtgtcgtca gctgcatggc
     3841 tcccagcacc cgaaaggctg cccgaagccc cgcgtcccgc tcccgctgca tggcgctggg
     3901 gccgtctacc tgcgtccggg tctgacgtgc ccggcggatc catgcgatca ttctttattc
     3961 ctcccgtcaa agcggcgtat ccgaccatag gcccgcttca tttcctccgg gttgctcaaa
     4021 taggtgcgca ggcgctgtcg gaaggtgggg gcgcgaagca gcaaatcggg gttatgggta
     4081 cggcgggggc ttcccggggc cagaaagggc tggcccaggc tctccatggc cgccacccag
     4141 cacaacagca tcactacggc atagcacagg ccggaaatcc ccgtaatgcc cgccgccagc
     4201 agcagcacca cctgccccat ccgaaaggcg aaggacagcg gatagttggg catggcgtag
     4261 cttcccagcc cggagagcgc caccacgatg atcagcaatg ggctgaccaa ccccgcgtcc
     4321 accgccgccg tgcccagaat cagcgtactg accagtccca gcgaggaacc catcatgccg
     4381 ggaatgcgaa tggccgattc gttgatgagg gcgaacaccg ccaccatcag gagggtttcc
     4441 tcaaacagat tgatgggcac gttggtacgg ctttgcatga tgctggtcag cagcgtcatg
     4501 gggatggtga tgggatgata caccaccaga ctgacgaaca ccgcgggcag cagcagcgtc
     4561 accagcgccc ccagcaagcg cagaagccgc atgaaggtac cgtactgcca gcgcatatag
     4621 ctgtcgtccg gcgcgtggaa caggtgccac aggctgacgg gaagggccag cgcccggggg
     4681 cttccgtcca gcagcaaaac ggcctgtccc tccatcagga agctgaccgc ccggtccggc
     4741 cgctccgtgc ccaccacctg cggcaggggc gcgtaggggt cgtcctcgat gagctgctcc
     4801 agcgtgccgg aggtcagcac gtaatccagc tccacgccgt ccagccgccg ctgaagctcc
     4861 tccaccgtct cccgggggca cacgccgtcc agatagcaaa gcgccgcctg ccccggcgtt
     4921 tccgtgccca ccttccgaag ctggcagacg aagttcggcg agggcagccg ccggtgcagc
     4981 agcgtcaggt tatcccgcag ggtctcgtta aaagcgtcat ggggccccac caccaccgtt
     5041 tcgtttcggg ggttggagac gggccgacgg gcataggcgc gcaggtcgat ccgcagggcc
     5101 tgatcgcagg tatccatcag caccagacac aggcgccgga gcagatcgtc cagcgcctgc
     5161 gagacctctg cgccgctttc cacctccggc acggtcagca cgctctgcat gacctgctct
     5221 gcggcgcttc gtcccgccag agcgagcggg ctgttcatca tgggccgaag aatatgctgg
     5281 gccatctgct ccccgtcgga aaggccctcc acatacaaca gagcgcagtc ctgccccagc
     5341 gcatcgaagc gtctcagaac cacgtcgtcg tttacgtcca gatggagccg ttcctgaagc
     5401 gccttcaggt ttttttccag attccgggaa aaaatcgtct gcatgtgtcn nnnnnnnnnc
     5461 accccgagca aatctacaga tgtctcattt ttcaaaagga cggacgaatc ttcatgacgg
     5521 tcgatcagtt ccgcagggaa gaaaacggcg gctatgcctg tcttcgtcct tataccgctg
     5581 aaattgcgga gaccatggag gactatcgga atatgcccga cgaggcattt gtaaaaaaag
     5641 cctacaacac cttcatgtcg ggcgcgaggt aaaaaagcat cggaaacacg accttgtttc
     5701 cgtgcacagg tgcgatttcc ggcttttgct gacagcgcag ttgcaaagca ggcgttgtac
     5761 aaaaatggat aaggagaatt atatggagtg gctgaatcct tttggactgc tcatcat
//
LOCUS       DAWAEA010000041         5795 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2180_length_5795_cov_1.608014, whole genome shotgun sequence.
ACCESSION   DAWAEA010000041 DAWAEA010000000
VERSION     DAWAEA010000041.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 5795)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5795)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5795
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2180_length_5795_cov_1.608014"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    214..342
                     /estimated_length=129
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2248..2257
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3687..3780
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4815..4843
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1059 a         1688 c         1524 g         1262 t
ORIGIN      
        1 cgctgaatga tggcctgacc tccgccctga tctccttcct gagtacactg gtgtttcagg
       61 tggcggccgt actgctcttt cccctgctgt gggggctgga cggcatctgg ttctccatcg
      121 tggcggcgga gatcatggcc gtgctggcca ccatcttctt cctgctgaaa aagcagaaga
      181 aatacggcta ttgatcctcc tattcaaaag cggnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncctgccta gttgatggag
      361 cgggcgtcgc tgaagggata gaccacgtat cggacgtgac ccaagatcat gtcccgggta
      421 atagcttcag gcacaccgtt ggtcaggttg cggcagtcgt ggctgttgtc gcggttgtcg
      481 ccgcagacaa aatactcgtt ctcacccagc gtaataggta acatgctgaa accgttctgg
      541 ttccgggacg gcgtcagata gggttcgtcc accacttccc cgttgcgaat caatacgttg
      601 tccacaaaca tcagcgtgtc gccgggaacg cccacgaccc gcttgacgaa attcgtgttg
      661 ccccggtcgg gataatggca gatgaccacg tcgccgtgct ggggatcccc cgtcagatat
      721 tccggcttgg tcacgaacat gatctcgccg ttggccagcg tgtcgtccat gctgtggccg
      781 tccacccgta ccggctcaaa gaggaaggtg cggatgacca aagcgataat gaccgccgcg
      841 cccagcgtca ggatccagct caaaatttcc tttttaacat tcacaggctt cttttccttg
      901 ccttttttcg tttcttctgc aggctgcaat tcctgttctt ctgccgcctg aacgttttcc
      961 gctgcattgt ccgtctgcat tcccgtgttc ctccttcgaa ttgcccggcc atctgccgca
     1021 gcgtttccgc ctccggcacc ggcccctgct ccagtatttt cttgcggcgc ttcacaaatt
     1081 ccggccgttc catcataacg acgcgtccgc agccaaggca tttgattttg atgtccgcgc
     1141 caatgcggat cacctgccag cggtcgctgc cgcagggatg ggccttgcgc gtctgaatga
     1201 cgtcgcccag acgaatttct tccacgctgt acgcacctcc ggcatgatga tgaaagaatt
     1261 atacaacctg accgcggctt ttttcaatcg ttgtactcca ccgtctgctg gtaggccttg
     1321 taagtgctgg tgtgcagaat ttcccgcttg aactcttcgc cattcttata ccagaccttg
     1381 taggtttcca ccacgtagcc cgtgcgggcc ttgacagtga tcttgttggt gccgtaagga
     1441 agctgagcgt tgtagacgta cttgacctcc gtaggcgggt ccatggtgcg gatgacctgg
     1501 gattccaagt cgatgctgac cccttcgccc agcgtcatgc cccaaatttc agcggacatt
     1561 ttccgatcct cgtaataggt gatcaggaaa accgggtagt ccgtgttgtt tttgaacttg
     1621 aaatccagat tgggccagtt gaccgtcgcg tcctcaccct tattgacata ggagctgggc
     1681 cacgcgtggg gactgcgctc cacgatctcc agattggccc tggccacggc gttgaaaatg
     1741 gtggaggaca cctgacagat gccgccgcct acctcctcaa tgctctgccc ggcggcgatg
     1801 gccccggcag acttgtagcc cttttccacc gtgcgctgcc cggtggtctg gttgaagctg
     1861 aaaatctcgc cgggcatgag cgccgtgccg ttgatggctt cgcaggccag acggatattg
     1921 gtgttgcggt tggaatcctt cgtagtggta gtcgtgtagg cggccaccat ggtaaagcct
     1981 gccgccagcg tgtccttggt gacggacggc tgggtgatga ccgggtccac ggtaacggtg
     2041 gcgttcttct cccacttgtc cagcgcggcg cagacccgct catagagcag atcggcgtcc
     2101 agcgtcacgc ccggctgttc gctggaaaag gtgaagctgc gggtcttgta gtcgaaggtc
     2161 aaaatttgcg cgtcgatggg atcgcgggtc acataggcgg cgatctcatc taccagcgcc
     2221 cgcaccgctt cgtgatcgta ggtagcgnnn nnnnnnnttc cagcacgttg cccagatccc
     2281 gggcagacgg cacattggac gaatcgaaat tccacgtttt atccccgata gcgaccgtga
     2341 cggaaaactg tcccgccgtt tcccccgtgg tcttagacag cgcttcccgg gcttcctcca
     2401 ccgtcatgcc gcccacgctg accccgtcta caaatacgcc gggatagatc acgttctggg
     2461 ccatgtaatt tttgtactga cggtaggaag cggcggcccg gctgtcataa cgcagcagct
     2521 cgccgttgat aaaggcgtaa tttccaaagt ccttccagaa atatcccgcc atttggggca
     2581 tcatcatcac gcccagcacg cacatgccga aaaagcacag ggcaatgatg aacacataca
     2641 ggaaattctt ccacggcttc gtttctctgc cccgcccgcg gtctttgccc ttcgccggtt
     2701 tccttctgcc cggttccggg cggcggtttg gctgtccctc cggcggccgg cttcccgccc
     2761 tgcgccgaac cggaagggac tggaccgacg cgcgccagtc cagatcgtcg tcgaaaagaa
     2821 actcctgttc ttcctcctca tagggagaaa ataacggccc gcccatggtc ttttgcagat
     2881 ccgccgggcg gacaaattct ccctgttcat tcacgatcag ccgtttcttt tcttccggcc
     2941 tcgtctcata tcctccgccc aatgccgcca tagccgtcct cctgctccat gggagaagcc
     3001 gccttctgaa ggattcggaa gctcagcact tccaggcttt catccagatg cacgttctcc
     3061 accactgcat tctccggcag gtacaggtct acgggcgcaa agttccaaaa cccgcgcacg
     3121 ccgcaggcgt acagtcgctc cgccagcccc tgtgcggcgc gggcgggcac ggccagcagc
     3181 gcaatatcca ccgggtgctc tcttacaaaa cgttccatgc agtcaatatc ctgcacgaac
     3241 aggccgtcga cctccctgcc gatctttttc tcatcccggt caaagatggc aatggtctca
     3301 aagccgttgc gtggaaagga atcggagcgg gccatggccc tgccgatatt gcccccgccc
     3361 acaataatca tctgatgggt ctgatccagc cccagaagat gaccgatatg ttcccgcagt
     3421 ccgctgacgg agtagccgtc cccctgccgc ccatagccgc cgaagcagtt gatgtcctgc
     3481 cgaatctggg acgcggtcag tcccatgcgc tggcccagtt ccttggagga gatctggcgg
     3541 acgctttccc gttccagctc cttcaaatgc cggtaatagg caggcagacg cttcaccata
     3601 gcgtcggaaa catacatgga tcattcctcc gatttctatt ttttcccgaa ccgccggatg
     3661 aatgcggcca gtcatggatg ggtcagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 aaaatgccga aagggttcat tcgccccaca ggccacgccg cagcgcgtcc gaacggttgc
     3841 ggaggcactc ctgcatgacg ctggtgcggg ggtccacgtc ataaccgtgg aacacgccct
     3901 tgatctcgtg cagctccacc gttccgcccg cttcttccag ccgccgggca taagccacgc
     3961 cttcgtcgtg caggcagtcc agctcggcca cctccatata ggtcaggggc agttgtttgg
     4021 gcagctccat ttccataggc gatgcctccg ggcaggtctt tccttccaga tacatgcgcc
     4081 acatttccgc gttgtgctcg gcgttccaca tgggcgtatc ggtatatttc ttcatggatt
     4141 cggtttccat gcgttcatct gccacgggat agatcagcat ctggaatttc ggcgcgggca
     4201 agccccgcat ttccgcgtcg cggcagaggt agatcgccag tgcgccgccg gcgctttccc
     4261 cgcccacggc gatgcgttcc gggtcaatat gcagttcctc cgcgtgctcg caggcccatg
     4321 cataggcggc aatatcatcc tcccgggcgg caggataggg atattccggc agcagccggt
     4381 aatccggcat cagcacccgg cagttggcct cccgggcaaa cagcgcgttc atccagcgat
     4441 gctggggcgc ggccccgaag ccgaagccgc cgccgtgggt atagatcatc agcggcagcg
     4501 tttcgtccgt gtccttgggt tcggagatca gcatctgcat ttctccgccc ggcgagggga
     4561 tggtgaccca gcgctgctcc accttgcggg aggaacctgc gtgcagaagc agctccatcg
     4621 gtttatggga caccttcaaa aataaagcgg taaacggtgt gtggaacggt tcaaacttac
     4681 ggtattccgg ctgcacccag ccccattttc ccatacgtcg tctcctttgg tttcaatatc
     4741 agggtcatac ggctcttttt tgaaagtgct cggcatgacg acctgtttcc cattataaaa
     4801 agttcccgtt tttcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaagctgg attttcccgg
     4861 ggcttcctgt tatgataaag atacgattta ttttctattt aggaggtaat tccatgactc
     4921 cccataacac ggctgaaaaa ggccagatcg cgtccacctg tctgatgccg ggcgatccgc
     4981 tgcgggcaaa aatgatcgcc gaaaatttcc tgaccgacgt ggagtgcttc aaccagaccc
     5041 gtgggatgct gggctacacc ggcaactacc aaggcaagcg cgtgtccgtc atggggcacg
     5101 gcatgggcat tccttccatc ggcatttaca cttacgagct gttctccttt tatgacgtgg
     5161 agaatatcat ccgcatcggt tccatcggcg gtctggacgc gtccctgaat ctgggcgatg
     5221 tgatcctggc acagggtgcg tccacggaca gcaactacgg cagccagtac ggtcttggcg
     5281 gcacctttgc ccccatctgc gactacggcc ttctgaagtt ggccgaccag acggcggaaa
     5341 agaaggggct gcccgtcaat gtcggcaacg tgctggacag cgacatgttc taccgcgcca
     5401 acccggaggt ttttgacaag tggcgcgcca tgggcattct gggcgtggag atggaatgct
     5461 ccgcccttta catgaacgcc gcctatctgc acaagaaggc gctgggcatt ctgaccgtca
     5521 gcgacatcat cggcgacgac agccgcgcca tgacggcgga agaacgccag accatgctga
     5581 cgaacatgat cacgctggcg ctggacatgg cgaaggagct gtaaggaagc agggctgtgg
     5641 ctttgcactt tcccggcggc tttagtattc gatccggctc atttttcagc atagcaacag
     5701 acggcacggc agtacaagcc gtgctgtttg tttttctaga ggaagaaatc tgctagcagt
     5761 ggtttgccga gtgtttgccc cgaggaaaga acccc
//
LOCUS       DAWAEA010000042         5721 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2214_length_5721_cov_2.956936, whole genome shotgun sequence.
ACCESSION   DAWAEA010000042 DAWAEA010000000
VERSION     DAWAEA010000042.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5721)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5721
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2214_length_5721_cov_2.956936"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    787..1015
                     /estimated_length=229
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5003..5012
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1059 a         1706 c         1718 g          999 t
ORIGIN      
        1 cgagcgggcg gggcgggcct ccaccgggcc gttttccgtg tgcagcgtca ggcggaaggg
       61 cgtttcctcc gccgccagat ggttcttgat atagttgtcc tgcgcgcctt cacagaccag
      121 ctccagccag ccgccgctga ccgcgtccag cgccagtgac acgaccccgt tggtcgcgtg
      181 cagtcgggag tgatacatag agggggcctc cattcagttg ttttgtcacc atgatagcgt
      241 gaaaacaagg cgttgtcaat ccttctggct cgatgtgaaa agaattgttt tgtcaggaag
      301 caacatatct acttgacatt tttctttcat ctccgtatga tgaaggggct gataaaacgt
      361 ttttttggaa gaactggata caacgcccca tttcattttt cacccagaag cgaggaggtt
      421 ttttcgatga aaaaggctac cattctcagc ggtatcgacc gtcttcagct ggcggacgag
      481 cagcttcggg gccgccgggt gggtctgatg accaacccca ccggcatgaa ccgggagctg
      541 aaaagcacca tcgacatcgt caacgagcgc tacaacctga ccgccatgta cgccgtggag
      601 catggcatcc ggggcgacgt gcaggcgggc ggcaaggtgg agacctgcgt ggatcccgag
      661 accggcgtga ccgtgttcac cgccttcggc gaaacgtccc atttcacgga cgaaatgctg
      721 gacgccttcg acgtgctggt tttcgacatt caggacgtgg gcgcgcgctt ctacacctat
      781 ctgtatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncccgc
     1021 tggagggctg ggagcggggc atgtatctgg acgacaccga cctgccgtgg gtagcgccgt
     1081 cccccaactg cgccacgctg aacgccgccc tgtgctacat cggcacctgc gtgttcgagg
     1141 gcaccaactt aagcgagggc cggggcacca cgctgccctt tgaggtcatc ggcgctccct
     1201 tcattgacaa cgccctgctg gaaaagaaaa tgaacgggct gggcctgccg ggcgttcact
     1261 tccgccgcac gtccttctgc cccaccttca gcaagcatca gggcgttctg tgtcacggcg
     1321 tgcagatgca tgtgctggat cgggagacct acgattcctt caccgacggc ctcctgctga
     1381 tggataccat ccgcagtctg taccccgagg agttccagtt cgtgcgctgg agcgaaaaat
     1441 ccccctattc catcgacctg ctgctgggca cggacgaata ccgcaccggc gcgctgacgg
     1501 cgcagggcct gatggacaaa tacgcgccca aagtaaagga atttgtggag gccgccaagc
     1561 agtaccggct gtattgaacc gcctgacttc ctacattttc aatcaggagg acttttttca
     1621 tgagttggac gttgaaaccc ctgacaaggg agcacatttc cgccgccgcg gcgctgttta
     1681 accagagtgt cgccgacggg gaaatgatct tctccccgct ggacgaagcg gtctttgaac
     1741 ggcgcttctt tcagcccggc gcgcacgcct tcgccgctga agaaaacggc gaactgatcg
     1801 gctttatcca cggcgcgtgc aagacggcat tcctcaacaa ggaaaacaac gaaaacaccc
     1861 ccggctatct gtccgtggtg gtggttcgga aggacaagcg gcggcagggc gtgggttccg
     1921 cgctggtggc cgcgctggag gacgctttcc gcgccgcggg caagcgcagg gccgcctgca
     1981 acgacagcac ccccgtccac atgaactgga ccatccccgg cacacccggg catgaccaca
     2041 acaacgcccc cggcgtggac gaacgctgcg aaggctatcc cttcctgctg caccggggct
     2101 atcaggccgc cttcacggag atcgccatgt atctggactt gagcaaattc cagtgggatc
     2161 ccgcgctgga tgcgaaaatc accgcgctgg atgccgaggg catccgggtg ggccgttgga
     2221 cgcctgactg cggcacggag cacgaccggc tctgcgacca cgtgggcagc gagtactggc
     2281 ggcacgtgct ggataccgag ctggaagcgt ggcagaaaaa tgagcccaac gccgacccgt
     2341 ccctctggcc ggacggccgc cgccctgccg ggccgcgccc cctgctgact gccaccaccg
     2401 gcgggcacat cgtggcctac accggccctg tggatctgca aaaaagcggc cggggctggt
     2461 ttaccggcat ctgctgcgac ccggactacg gcggccgcgg catcgcctcc gtcctgttca
     2521 acctgctcat gcgggagttc gtggccgagg gcgcgaagtt cagcaccctt ttcaccggcg
     2581 agaccaactt cgcccagaag atctacagcc gcgccgggtt ccacatcgtg gcccgctttg
     2641 tggggctgac caaggaactg tgatccatcc atcctttctt ttcggggact gcttccgccg
     2701 cgccaaggcg caggggaggc agcccctctt tttccgtgct tttccgcccc ttcccccgcc
     2761 gggaaaaaga tgctataatc ttttctgcta gagcgttccc tcatccgggc cgcttttcct
     2821 caattcacca aggaggtttc ctcaaatgaa cgaaccgatt ttgggcgtta ttttcgatct
     2881 ggacggcgtg atcgtctcca ccgatgattg tcactatttg gcttggaagc gcatggccga
     2941 cgaagaaggc attccctttg accgcaaggt gaacgagcgt ctgcggggcg tgagccgtat
     3001 ggccagtctg gagatcattc tggaaaaggc gacgaagacc tacaccgagg agcagaaggt
     3061 ggaaatggcc acccgcaaaa acggctacta tgtggagctg atccagcagc tgacggcgga
     3121 cgccatgctg cccggagcca tggccacgct ggagatgctc aaaaagcgcg gcgtgaagat
     3181 cgccattggt tcctccagcc ggaacacccc catcatcctg cggcagatcg ggctagacgg
     3241 ttacttcgac gcagtggccg atggcaacca gatcacccac agcaagcccg acccggaggt
     3301 tttcctgctg ggcgcgaagc tgctgaacct cgaccccaaa aactgcatgg tggtggagga
     3361 tgcggacgcg ggcgtggagg cggccctgaa cggcggtatg cgggtgctgg gcgtgggctc
     3421 cgccagcgcg aaccccaagg ccacctttac cgccgccagc cttgcggacg cggactttga
     3481 cgaaattctg aaataactca tcccaagcga gaggagaacg accgaatgtt agacaagcat
     3541 cttgccatgg aagcgctgga cgccgctcta tccaccggcg gcgactttgc ggagatcttt
     3601 gcggaggacc ggcgggatca ggccgtagcc ctgcaggaca gccacgtgga gcgggtgaac
     3661 tcgggccgtc tgcacggcgc gggcgtgcgg gtatacgacg gaacgtccgc cgtatacgcc
     3721 tacaccaacg attttggccg ggacggcctg ctgaagtgcg ccaaagaggc ggcttcggcc
     3781 atccgcgccc ggcggcaggg tcgcacggcg gttcagccga tggaggagcg gcatttcgtc
     3841 aaccgtcatc ccatctggat gaagccctcc gaggtggaca ctcgccggaa gatcgacgtg
     3901 ctccgggcgg cggacaaggc ggcccgctcc gcaggcgagg aaatctctca ggtgctctgc
     3961 gccttcaccg acagcgagca gaacgtgctg atctgcaaca gcgaaggcct gtacgtcgcc
     4021 gatacccgcg tttattcccg cctgcgctgt caggcggtag cctccaacgg cacggagaat
     4081 cagtccgccg gtgaagcgcc cggcgcggcc cggggcttcg actatttcct tgacaccatc
     4141 gacccggctc tgatgggtct ccgggcgggc aaaacggcgg tgaaaatgct ccacgcgccc
     4201 ttctgcccct ccggggtgat gccggtggtc atcggcagcg gctttggcgg cgtgatcttc
     4261 cacgaggcct gcggccacag tctggaggcc actgccgtag ccagcaacat gagcgagttc
     4321 tgcggcaagc tgggccagca gatcgccgcg ccctgcgtca cggccatcga cgacggcacc
     4381 cttcccaacg aatggggcag cgagaacgtg gacgacgagg gcatgcccac gaaccgtctg
     4441 gtgctgatcg aaaacggcat tttgaagaac tacatgatcg accgtctgaa cggccgtcgg
     4501 atgggcatgg cccccacggg cagcgcccgc cgggagagct acgcctacgc gcccacctcc
     4561 cggatgcgga acaccttcat tgccccgggc tgcgacgatg aggcggagat gatcgccacc
     4621 atgggcgacg gtctttacgc agccagcatg ggcggcggtt cggtgaaccc cgccacgggc
     4681 gagttcaact ttgcggtcaa cgaaggctat ctcatcaagg acggcaagat cgcccacccg
     4741 gttcgggggg ccagtctggt cggcaagggc ggcgacattc tcctgcaaat cgaccgggtc
     4801 ggcaagaata tggtcatggc gcagggcatg tgcggcagca tgagcggctc cgttcccgtc
     4861 aacgtcggcc agccctggat ccgcgtcaag tccatcacgg tcggcggccg gcagcagggc
     4921 taaagccctg cacccgtcac tgcgcggctt tgccgcgcag gggcgtagag gacttgcagg
     4981 ttggccgggg ttttaccgcc gtnnnnnnnn nngctcgccg gtctggtgtg gattcccgct
     5041 ggttggccgg tacagaccgc cactcgacag aaggtctcgt ggcttgcgca aggggcagtg
     5101 ccccttgacc ccagactgcg tgctattcgc gcaggggcgt aagaagacat tcggcttggc
     5161 cggggtttta ccgccgtggc acgacgggtg ccacggctcg ccggtctggt cggcgaaatg
     5221 ctggttggcc ggaaatggcc gccgctcggc ggaaggtctc gcgcaagggg cagtgcccct
     5281 tgaccccgga ctgcgcgccg caggcgcgca ggggcgtaga gggacttgca ggttggccgg
     5341 ggttgtaccg ccgtggcacg acgggcgcca cggctcgccg gtctggtgtg gattcccgct
     5401 ggttggccgg tacagaccgt cgtcgggcaa caggccctcc ggctcgcagc aagggccagt
     5461 gagcttcaga aaaaacgttg gctctttgcg ttgcagaaat atccaacttc atgtcgcaac
     5521 attccaaccg ccattatgag tatggtttat accatctgct gaatcaaaac caagcctgtt
     5581 tttctgtgct gcggcatcat cccagcacac cgaagcatcc caagcatcgc catttaagtc
     5641 tgcccagcgc caactgtggg gaaaacgagc ggcgataggc agcggaggga aaaaggcccg
     5701 cccattttga cggcggaaaa a
//
LOCUS       DAWAEA010000043         5634 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2266_length_5634_cov_2.089622, whole genome shotgun sequence.
ACCESSION   DAWAEA010000043 DAWAEA010000000
VERSION     DAWAEA010000043.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5634)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2266_length_5634_cov_2.089622"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    330..406
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4856..4937
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1116 a         1647 c         1688 g         1024 t
ORIGIN      
        1 tgtcggattt ctgccgggcc gtcaggacga gcgccgcttt gatatgaccg atattttcct
       61 gaccgacgag gaaatcgagt tccccaaagc cgccgaaacc accgtggatg cactgggcca
      121 gtggaagcag aaggattggc cgggaaagct tccctctgcg gcggcgctga aggacttttt
      181 gcagacggag tggaaaaaag agcagccgcc cctgccggga cggaaccgct tcggcggatg
      241 cacggagcgg cagctggaag ccacgggctt tttccacgtc cagaaggaag acggacgctg
      301 gtatctggca gacccggacg gctgcgtgtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggct ggattcgcgg
      421 cgtggaagac cagatggacg atctgccaga cccaaggggc cccttcgccc cggcctatga
      481 gctggccgag gaaaacgagc tgtaccgccg aaaattttcc ggcatgttcc cgaaggatac
      541 ccttctgtac gccccagcca cggccaatct gatccgcgct ttcggcggag aatggtatga
      601 ccgctggtgc ggactcaccg cccgccgcct gcgggcgtgg gggatcaaca cgctgtccat
      661 gttcagcgac ccggagttca tccgccgctc gaagatgccc tacgttatta tgctcaaggg
      721 ctatcccgcc acccggcgca tgatctaccg ggacttcccg gatgtgtacg ccgaggaata
      781 cgaagaaggc tgccgccggt tcgccgccca gctgacggcc tatcgggacg atccgctgct
      841 gatcggctat ttcctgaata atgagcccac gtggggcttt gtggaaggca tcctgctggc
      901 ggagcgggtg ctggagaacg ggcagggcac cgctaccctg cgggagctga tcgcctttct
      961 gcgccgacgg tatggggagg atatttccgc gctgaaccgg gcgtggcggc tggagctttc
     1021 cgattttgaa acgctggaaa agggcctgtt ccgggcggcg gaacgctccc cggaagccga
     1081 acgcgacctg tctgacttta ccggccagat gatcgaccgc tatgcggggc ttcccgccca
     1141 atacgcccgg gaggtcgcgc cacgtcacct gaatctggga atgcgcttcg ctcatgtgaa
     1201 ccatcccgga atgctgcggg ccgcaaggca tttcgacgtg ttctccctca actgctatgc
     1261 cgccgacccc acggaagcgc tgctggcagt gcaaaaggcg ctggatatgc cggttttgat
     1321 cggcgagttc catttcgggg cgctggacgc gggattgccc gctcccagcc tgttccatgt
     1381 gaaggatcaa aaggcccggg gcgacgccta cgcccggtat ctgacccggg cggcggctca
     1441 gccgtgcagc gtaggggcgc actacttcgc ctataacgat cagccccttt ggggccggta
     1501 tgacggcgaa aattatcagt tcggctttgt ggacgtgtgc aaccgcccct acgagccttt
     1561 cggcgagggc gtgcgccggg ccaacgaaga aatttaccgg gtcaaggagg gacttgccgc
     1621 gcccgaggac ggcgagaccc aacgggaatc gtgaaaagat ccattcgaaa atacaggagg
     1681 caacgcccat gtttcagaaa accaaagtca cggatgaaat tcgtctgatg gcggcttacg
     1741 actctcccgc cgccgaagaa atgcccatgt tcgcggaaaa ccgggtgcat cagcgctcca
     1801 gcggcaatcc ctatcccaat aaggtggtca tgcgggtgga tcgggcccac cgttccgaaa
     1861 agccctttcg aatcatcaca ttggaaaacg aatatttgaa gatcgagctt atgcccgaac
     1921 tgggcggccg catctacgcc gccatggata aacgcaccgg ctacgacttc ttctaccggc
     1981 agcacgtggt gaagcccgcg ctgatcggcc tgctgggcag ctggatctcc ggcggtctgg
     2041 aatttaactg gccctgtcac caccggccca gcacctatat gcccgtggac gtgcaggtgg
     2101 aggaggaaga aaacggcgcg gtcacggtgt ggatgagcga aaacgagccg ctgaaccgca
     2161 tgaagggcat ggtgggcgtt cgcctgtgtc ccggagaagc acggttcgat acccggatga
     2221 aggtgtacaa cggcacgccc gcccggcact ccttcctgtg gtgggaaaac gccgccgtgc
     2281 ctgtgaacga gcagtaccgg ctggtgttcc cgccggacgt gcattacgtc cagttccact
     2341 accgcaagaa tgtgacggaa tatcccgtgg cctccggcgt gtacaacggc attcggatgg
     2401 gcgacggcac ggatatttcc tatcataaga acacgcacca gcccacgtcc tatttctgcg
     2461 cggccacgaa gtacgatttc ttcggcggct acgacgaagg caagcgctgc ggcgtggtgc
     2521 atgtggctga ccgccatgtg agcgtgggca aaaagatgtt cacctgggcc tacaatcagc
     2581 tgtcccgcag ctgggagcgg gcgctgaccg acaccgacgg cgcttacgcc gagctgatgg
     2641 ccagcagcta ctccctcaat cagccggatt tcgcgtggct gtacccctac gagagcaagg
     2701 aattttccca gatgtggtac cccgtgggcg acggcggcgc gcctttctgc gcctgccggg
     2761 aagcggccct gtccgtggaa agcggccgtc tgcggctgca ggcctctgtt cccctgcggg
     2821 atgcgaagct gatcgtcaac ggcgcggagc accgcttcac cgccgggccg gagcaggctt
     2881 tcgacctgcc tctgcccgag gagatcgccg cgttccagct ggtagacaac cggggtaaga
     2941 cgctgctgcg ctatgaaaag cctgtggaaa acgaactgaa ggaaatgccg gagaccatcc
     3001 cggacaatcc taccatggat cagctcaaga gcgcacagga attgtatctg ctgggcgtgc
     3061 atacggagca gtaccgcgac ccggccattc gtcccgccgg gtactggcgg gaagccctgc
     3121 gccgggaccc ggatcatctg ccctccctga tcgcgctggc caatgacgag ctggcccact
     3181 tccgcccgga gaatgcccgc aagctggccc ttcacgcgtg gaaggtggcc acggtgcgca
     3241 atttccatcc cgaaagcggt gagctgcaat acgtcatggc ccgcatcgag gaagcactgg
     3301 gccgggagga cgaagcgctg gacagctatc tgcaggccgc gtgggcgcag gacgcgcgct
     3361 cccgcgccat gacccgggcc gcgctgatcc agctgcgccg gggacagtat gacgatgcgc
     3421 tgaccagcgc ggaggaagcg ctgaaccagc acgcccataa cgagaccgct ctgctgatcc
     3481 gctatctggc gctgaaaaag ctgggcgacg accgtgcaga ggaagcgctg cgccgggcgg
     3541 aggcctatga cagcctgaac ctgaccctgc gggcgctgaa atacggcgcg tctcccgagc
     3601 tttacgccac gctggaaagc gacccctgcc agtcggcgct ggatatggcg caggatcttt
     3661 ccgaaatggg ggagacggag ctggccgcag ccattctggc tggtctgccc accccatgcg
     3721 cccagacgga gtacgtgcgc tggcacctga ccggcgaccg ggcggcgctg gaaaaagccg
     3781 ccgggctgga agcgggcgcg gcttatcccc tgcgggagat tgagcaccgg gcattgacgg
     3841 aagcagtgaa ggcgcatccc gaggacgcga acgccctgaa tctgctggcc tgtctggaat
     3901 accatatgga aaaccacgaa aaggcggacg aattgtggag ccgcgccgcc aaggccgacc
     3961 ccaacgccta catgcccctg cgcaatctgg cggtggctcg ttacagccat ctgaaccgcc
     4021 gggacgaagc cctgcctttg ctagacaagg cgcttgccct gcatcccggg tacggccagc
     4081 tggtgtggga aaaggcttat ctgatgaccc gtctccactg cgaccccaac gaaacggccg
     4141 cctttgtgga gaagcacggt tacgagcggg aggatatcgc catcgaactg tgccgggcgc
     4201 tgaatctggc gggccgctat gaagaagcgc tgaacatcct gctgggcaag aagttcacgc
     4261 cctgcgaggg tggagaacac gccgtggccg agcagtatat gttcgcccat cacgcgctgg
     4321 gccgggggct gtttgcggat ggacagtacg aacaggcgct ggagcatttc cgcgccgctc
     4381 agaccctgcc ggaaaatctg ggcgcgggcc tgtggaacga ggtactgctg gtgccccacc
     4441 agtatttcga ggcccgctgt ctggaagaac tgggccgcgg ggacgaggcc cgggcactgt
     4501 acgatcatat tctgatcttg aaggtggact atttctccaa catgaacctg cccgaactgc
     4561 cctgctggca ggccatggcg ctgaagcgca cgggccgggc tccgcaggcg caggagatga
     4621 tctccgccca cctgcacaag caggaaaacg ccgcctttgc ccgggatgcg ggctactaca
     4681 agaccacgcc cttctttatc tcctatatgg aggacgcgca gaccctgcgc cgggccgggt
     4741 gcgactggca gtccgccatg gcctgctggg cggcgggcga ccggcagacg gccgccgaac
     4801 tggctgcgct ttccctgatc ggcgagccct ccaacttgta tgccctgctt ttgagnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnctt tgccgctccg gcacagggct gtttttcgtg tgaaagaaaa
     4981 gttttgttca atttctaaaa ttccttattg acaagtattg ttcaccctgc tataatcaag
     5041 gcagatggaa actttggcaa cgaaacgacc gttccgggta tggaaaatca ttcctttgtg
     5101 aaaaggtgct tgcatcttcc ccggaaagtt gttataatta ccacacagac gaaaaaacat
     5161 tgcgtttgat tcgaatgggt ttccaaagca aagttgcttt tcctcttttc cattaaaccg
     5221 gtttgatggg aaaaaggtcg aaaagcaaga cgtttctttt tttatttttc atacggaaag
     5281 gagcccttgc cccctcccgc aagcacgtgg caaatcggat ctggcaaaac cgttacattt
     5341 acatgatggt catcccggta ctggtgtaca tgatcctttt caagtacatg cccatgtact
     5401 tcctgcgggc ctccttctac gactacaagc tgctcaaggg ctttgaaggc tccaagtttg
     5461 tgggcctgaa atggttcgag cgccttttgt ccagccctga tctgtggcag tacatcaaaa
     5521 acaccctgtc gctgaacctg ctgtcgctgg ccatcgtctt ccccgcgccg ttggtgttcg
     5581 ccattctgct gaatgaaatt cacagcgcgc cctacaagaa gatcgtccag accg
//
LOCUS       DAWAEA010000044         5602 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2291_length_5602_cov_2.200829, whole genome shotgun sequence.
ACCESSION   DAWAEA010000044 DAWAEA010000000
VERSION     DAWAEA010000044.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 5602)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5602)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5602
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2291_length_5602_cov_2.200829"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    369..378
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1022 a         1874 c         1562 g         1134 t
ORIGIN      
        1 gtgtttccag tgttcccacg cactgcggcg catccgctcc gtttcctcct ccgtcaggga
       61 ggccagttct tccagccgtt tcagcacgcc gtaagcccct tcttccggct ggtacaggaa
      121 gccgatgtcc ttcgtcacca gttccgggat gccgcccacc gacggggcga tcaccggcac
      181 gccccagcgc atggcctcca tgatggagac cggcacgcct tccttttgac tggtgttcag
      241 aaacacatcg aaagcccgct tttcgtaaaa tttttctact tctttcgcgt ccagatcgcc
      301 cagcagttca taaaggatgt tatcctttga gtccagcttt tccgccgcca gccgccgcag
      361 ctccgtttnn nnnnnnnngg gcattcccgt ccactgggac agcgcctcca ccaccatggg
      421 cacctgcttg atgggcacca gccgggcgca ggtgatgatg cggaaaatgc cctgcgcaaa
      481 ccggggtgcg cgcttccagc tttccaccgg atggccgcca gagccgatgg acaaaacatg
      541 catccgcttt tcgtccacct tgccgcgcag gtattccatc atctgctttc gggccacccg
      601 gctgatggga tacagcccgt cggcctgcgc ggcgataaag cccttcatca gataggggtt
      661 cagggggttg cgttccgtgt cgatgtcata ggcatggccc cgggccacga agcgcagccg
      721 gggatgtttc cgcttggaca gggctgcggc ataagcgtcg tagctcatcc agaaggaata
      781 cagcgtggtc tgcgaggcca cgcagcttcg cagcagcttt tccgtccagt agtgcatttt
      841 cagcccccgc acccggaaca gatagagctt gagaaagttc ttcagcgtca gcttgccatc
      901 cttgcgcagg tggttcgcct cctgccacaa ttcccggcaa aagggcgcgc cgagccatgc
      961 ccgcagcttg tccagcccgg ttcgggccag cggaagctcc acgccgtccc ggttcagatt
     1021 gctgaacccg gtatggctga ccacgaacgt ccgctcaaaa tgctcggtga gccatcgggc
     1081 ctcctgcatc aagaaggaat tgttcttgcc attgggcagg acggcgcagt tattcagata
     1141 gatgaggttt cgttccatgg tatccccagc ctttctagta cgggtttgaa ctgctgttcc
     1201 caccccagat gttcccgggc ataggcccgc atccgggcgg gaagttccgc gtcaaggcgg
     1261 ctttctttgg cgaagcgcag aatttccgcg ccgtcgggaa tggtctcgtc atccggcaga
     1321 tggcggcaca tccgctcgtc ctccggcata tccgggtcgt tgacggcgta gaagaagggc
     1381 agcccccggg ccatgtattc ccgctgcttc aagggccggc tgacgatctg tcccagccgg
     1441 tggatgccaa gcccgcccat gccgatatca caccggtcgg tcagctcgtc cagcgctgcg
     1501 ccctgtaccg agccgagaaa ctgcgctttt tcgcccatgt tcagttcctg cgccagcctt
     1561 tgccactctt ccagtgaacc gtcgctgctg ccgcccgcga aatagatcac atagtcctcc
     1621 tgcaccgggg ccatggcccg cagcagccga tcgtagccct gcccccgggt catactggcc
     1681 agcgccagaa tagcgggcgg gcccgcctcg ccccggctcc tgtgcagggg caggctgtcc
     1741 gcgtccacgc cgttggtgat gttcatggcg gggcggccgt tcagcgtccc gccggcttct
     1801 tcgccgatca gcgtgtacag gtcgatcatg ggctccacct gccggaaaac ataatcggca
     1861 tagcgaaata ccggttcccg cagcaggttc gtctttttgc cgttttcgaa gggataggtg
     1921 ggatgctcca tcaccagctt ggccccgctt tccttcacag cctgaaaggc ggcgggcgcc
     1981 ttggaaaacg tcatcatata gcgcatatag accagcgcaa agggcatctg gcgaacggcg
     2041 tttttcatgc cttccatcag atcgtcaaac aaaaacgtat ggctgtaccc cggcagccgg
     2101 gaaaaccggc tgcggtgcat cagcttcttt tcctcccccc ggcacagcca gattcccgcc
     2161 tcatcccagc ccagatacca cgctcggtgg cccagccgct ccatggccgc catctgtccc
     2221 tgaaacttgg ggatcagcgt ttcctgaccg ttcagggggt atttcatcac aaacagaata
     2281 ttcgccatca gccttttgcc tccagctctt cccggtacag cgccgccatc tgctcaagca
     2341 ccgcgttcag actatagtcc cgaatcacgc ggcggttgcg ctcccccatc tcccgccgca
     2401 cctccgggtg ctccagcaga aagtgaagat cctgtgcgaa gccctccgcg tccatgggcg
     2461 cgcgcagcat tcccccgtcc atggggccga tgagatcggc attgccgcgc acgttggata
     2521 ccagacaggg caagcctgcc gccatggcct ccatctgggc catgggaagc ccttcctgca
     2581 gggacgggaa cagaaacacg tccgaagcct gcaaaagagc gggcacatcc gaccggaagc
     2641 ccagaaaatg cgtgcgctcg gccatgccca gcgccgcggc cagctccctg agcgaatcct
     2701 cccgctcccc cacgccgcta aaaaacacct gcacgacctt ctccccggca caggcccgca
     2761 gcacggtctc gtggttcttg cgcacgctgt gttcgcccac gcagatcacc atgggggcgt
     2821 ccggcccaat gcccaattct gcccgcacgg ccgcccggtc cgccggtggc tcaaagcggc
     2881 tcagatccac gccgatccca ttggtgcggg ccacccgccg ggccgggaag cgccgggccc
     2941 gggcctcgtc ctcccggttg atggtaatca gcaggtcggt gaaccgggcc agaaaccgtt
     3001 ccgccgggta gaacagcagc cagttgcgaa gcggcgcacc ggtgaaaaaa tggaacccgt
     3061 gggccgtata caaaacccgg gttccccgct tccgggctcc ccgggccgcc agacggccga
     3121 ccatgccgcc tacgggcgta tggcagtgaa tcagggcgta atcctcccta tccaccagct
     3181 ttttgatttc ccgatacact tgaagattct gccgtccgaa gggcgaacgc tgaaagggca
     3241 ggtcaaaaaa acggtcgcag taaggcacct ccgtcaccgg cttttcggtg tcgttgcagc
     3301 tgcacagatg cacctcgtac ccctgctcct gaaaccattt catatagggc aggtggaata
     3361 ccagcacatg gccgcgcagc atatgggcca caaacagcac cttttgtttc atgtttgctc
     3421 ctccgtcagg gcgatggagg aagcaaaatt ccattcttcc tccccgcgga aacattcgct
     3481 catggcctga tcgtaggtca ggctgccgaa taccttgccg aacagactgc ttctccggcc
     3541 aagggcccac agcaatctcc cgggccagtc cgtcggtaga gacatattcc cggttctggg
     3601 gaaaatacaa tccgccctgt ccgctttcca gcagacggcc catgaacagg cacagattgc
     3661 ctatatacag catactgcgc tgattgccgc agcggggaaa catcggcgtt ttcaggatca
     3721 ggcggcgcag gctctgatag ttgccccggc agccctttcc gtaaatcatg ggcgggcgca
     3781 gcactgccac cgtgaagcgt tcgtccgcca gcgcggcgat gtcctgttcc gcagcccatt
     3841 tgctggcgcc gggtctccgc tgtgatgtgt cccgtcaccc tgccgtaaac gctcatggtg
     3901 ctgaaaaaca cgaactgccg aacgccctgc gccttggcgg cccgggcgat ctccacggcc
     3961 agccggtggt tcacctgctc gtacaggggc ctgttttcag gcgtttcctt tcggtgggca
     4021 atgcccgcca cgtgcaccac ccggtcatag ccctgaaagg ctttttccgg cagcggcccg
     4081 cgcacgtcca gctcatccgc ttccacgccc tgcttccgca gcgcgcccaa aatcgcgccg
     4141 cccagatagc tccccgcgcc ggtgatgagt acccgcatta cggctgtccc tccgcccgtt
     4201 tttctttggt ttctcccgtt tcatcggagg gcttttcttc cttgccctcc acgatgccct
     4261 gccggttgat gaccttgacg atggtcagca gaaagcactt cagatcgaac cagaaggtga
     4321 tgtgccgggc gtactcgccg tcccggcggg ctttcttttc cagatcgcgg ctgagataat
     4381 cccggccgtt cacctgcgcc cagccgctga ggccgggacg cacgtcgttg gccccgtact
     4441 gatcccgcag ggcgatcagg tcatactgat tccacagggc gggtcgtggg ccaatcaggg
     4501 acatttctcc cttgaggata ttccacagct ggggcagctc gtccagactg gtcatacgca
     4561 ggaaacgccc caccggcgta atataactgg tagagcccgc caaatcgttg gtgggcacgt
     4621 cgtggggcgt atcggaacgc atggtgcgga atttataaat gctgaacagt ttcttgtgca
     4681 tccccacccg cttctgggaa aagatcaccc cgcccgggct gtccagccgg atcgccagcg
     4741 cgatcagcaa aaacagcggg cttagaacga tcagggcgat cagggacagg atcacatcca
     4801 gacagcgttt cccatagcgt tgatacatgc gcgttttctc catctcttgt ttgcttcgtt
     4861 gccttaaggg agacccagct ggccctccag cttccgcagg gtcttttccc cgatcccccg
     4921 gacgcagagc aggtcttccc aataacggaa agccccgttc tgttcccgtt cgccaatgat
     4981 ggcggcggcc agcttggggc cgatcccctt gacggtgcaa agctcctccg cgtccgcctc
     5041 gttggcgctg acgcggccct cggcggtgga ataagacagg gtctctcccg cccttgcggc
     5101 ggctttcggg gcaaggttcc acgccacatg ccccagcttc aagcgcgaaa gcgtcagagc
     5161 gcccgcgccc aaagccagca gcagcgccag aagtcccgtc agaatctgct tggttttaca
     5221 caccgctctt gcggactttc tggcccgcag cggtatcttc cagaatatag cccatggcct
     5281 tgatctggtc gcggagctcg tcgctgcgct tccagtcctt gttcttacgc gcctcagccc
     5341 gctcgtccac caatttctgc acctcggggt cgatgccgtc gtccttcttc atcaaaaggc
     5401 ccagtacatc ggtcatgccg aggaaggtct cctgtccaaa ttccacagcc ttgcggctca
     5461 cgtcggaagc gtccagcttg aagacctccc gcaccagctc gaagatggag ctgagcgcgt
     5521 cggcggtgtt cagatcgtcg tccatggcgg cgatgaaatg ctccttcgaa gcggtgcagg
     5581 cgtccatgaa ggccttttcg gt
//
LOCUS       DAWAEA010000045         5591 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2298_length_5591_cov_2.097182, whole genome shotgun sequence.
ACCESSION   DAWAEA010000045 DAWAEA010000000
VERSION     DAWAEA010000045.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5591)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2298_length_5591_cov_2.097182"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3459..3468
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1072 a         1671 c         1648 g         1190 t
ORIGIN      
        1 cttctacgcc gagcgggagg ctttgctgac cgagatcggc gtagacatgc gggaatttga
       61 gcgggtggtg ctgctttccg tcattgaccg ccactggatg gatcacatcg acgacatgga
      121 caacctgcgg gatggcatcg ggctgcgggc ctacggccag cagaacccgg tacaggagta
      181 ccgccgggag agctacgaca tgttcaacga catgatccac ctgatccggg aggagacggt
      241 acgccgcatt taccagagcc gaatccagcg cctgccggag cggcggaagg ccgtcgatct
      301 ggaaaagacg caggccggtc tacagggcga gaacacgaac ggtcaggcga agaagggcgg
      361 cgcgcccgtg aacaagtcgc agcccggtgc ggtcggtcgg aaccagccct gcccctgcgg
      421 cagcggcaaa aaatacaagc actgctgcgg gaagggctaa agcccttcac ccattaccga
      481 tctggtcgaa ctgaatgccg attggcgata caaggccgcc gctcggcgaa gggcctcgcg
      541 gctggcggga aggggcgctg ccttttggcc ccggactgtg cgccgatggc gcacagggtg
      601 ctcaggggaa agcaggttgg cagcgtttac cgccgtggca caacaggtgc cacggctcgc
      661 cggtctggtc gaactgaatg ccggtcggcg atacaaggcc gccgctcggc gaagggcctc
      721 gcggctggcg gaaggggcgt tgccttttgg cccccggact gcgtggcacg atactacata
      781 catctaccgt ctcagaaagg aaatggaaga attatggcga tgattgatac ggaacgatat
      841 gctcaggagc tgagcaaact gcgcgagacg gtggagaagg ccgccgatgc gctgcatctg
      901 cctcagctgg aggaggagct gaccgagctg aaggaagaaa tgaacgaacc cgaattttgg
      961 aacgatctgc agcgctctac gacggtcaac cgcaaggtgg gacagattga gggcaagatc
     1021 aaccatgtag cccgtctgcg ttcccgggcg gacgacatcg aagccatgct catgctgctg
     1081 aacgaagaac ccgacgagga aatgggtgcg gagtgcgaaa aggaactggc cgacctgacg
     1141 ggcgatgcgg acgctctgga gctggaaacc atgatgcgcg gcgaatacga cagctgcaat
     1201 gcggttttga gcctccacgc gggcgcgggc ggaacggagg cgcaggactg gacatccatg
     1261 ctatatcgaa tgtacacccg ctactgcgaa cgccacggct tcaaggtgac ggtcaacgac
     1321 attctggaag gcgacgaagc gggtctgaaa agcgtgacct tccaggtgga gggtgaaaac
     1381 gcctacggtt tcctgcgcag tgagcgcggc gttcaccgtc tggtgcgtat ctctcccttc
     1441 gacgccaacg cccgccgcca cacttccttc agttcgctgg acgtggcccc cattctggag
     1501 ggcgatcagg acttcgaggt caacatggag gaagttcgca tcgacaccta ccgtgccggc
     1561 ggcgctggcg gtcagcacgt taaccgtacc gactccgccg tgcgtatgac ccatatcccc
     1621 accggcatcg tggcccagtg ccagaatgag cgcagccaga tccagaaccg cgaggtctgc
     1681 ctccgcattc tgaaaagccg cctgttggag ctcaagcagc gggaacgcga agaacagatg
     1741 agcgacatca agggcgaaat gaaaaagatc gaatggggca gccagattcg ttcctacgta
     1801 ttccagccct acaccatggt caaggatcac cgcaccggct atgaaagcgg caatgtggac
     1861 gacgtgatgg acggcaatct ggacggcttc atcaccgcct atctgaagat gcagtaatgc
     1921 attttcagca gggaaaacca cgaggattca aggaaaatcc ggaaaggaag ccatcctgtg
     1981 aaagctgacc ttaacgccgc ggcgcgccgg gagcgggtgc tccgctatgc aagctacgga
     2041 ctgatggtgc tgtccccctt cctgctgctg ggggttcttt cgttgatttt gggccgcagc
     2101 gcgttgaatt cctggcccgt ctggacggat gaactggact aacgccgtcg gctttgccac
     2161 gggctacagc ggcctttcgg agatcgttcc ggaggccggt accctgagcg ttcacggcct
     2221 gacggccatc gtgttctatg gctggttcgt caagctcttt ggactgagcc acaacaccat
     2281 tgtactgtgc aacgccctgt gggtctcgct gggggcgctg ctgttctgcc tgctgctgaa
     2341 gccaaagccg ctgacctgcg tgtatatcag cgtcctgctg gccacctatg cgccggtggt
     2401 gctgtatgcc ttcacctcca tgacccagct gtgccagtac ggcgttctgc tgatctatct
     2461 ggcctttctg ctgcggtatg ccagaaaccg caagccgggt tggctgtttc tgtctctcgt
     2521 cagcgtggtg ctgatgtgct gtttccgcat caactatctg ctgctggtgc tcccggtggc
     2581 atgggtctgc ggcggcggta aaaagctctg cctgaaaacc gtgctgttca gtctgctggg
     2641 gctatgcctt gtcattggcg tttatctgct caccagccag tggtcggctc cctatgccca
     2701 aggcttcccc tatgccctgt ctcaggcggg cagctttgcg ctgatggtgc ggatgctcat
     2761 gagccatttc aaggccaatc tgatcggctt ctttgccttc taccgctaca gccccgtcct
     2821 gtggtggttc cgggtgttct atctggcgat gctggcgctc tgcctgttca aggccttcct
     2881 gcaggtccgc aaggacggta agggcctgaa attgggactg aacgccgcct cggtgtggtg
     2941 cttcctgtgg ctggccagcg cgctgggcat cgtgcttctg ttcaatgatg taaacgcatc
     3001 gacacggatc gtttcgaccc gcctgcggcc aatgaaagcc tgtccacggc catgagctat
     3061 atcacctacg accctgcggc ggacgatccc tttgacaata ccgtccgcac cgatctgagc
     3121 ggcgaacggc tcctgatgga gctggacgag gggctggggc tgcagtacgg ctggttcacc
     3181 ccggacagtc tgggcaaaag ccggtggatc ctgaccgata cactgaaggg agcgctggaa
     3241 ggctacgagc aggtttattc cacccacgga gccgccgtct accgcaagac cgaagcgccg
     3301 aaggagcctt aaatgacgca aaagaaacca atcgactgga aaacatggct gcttctgctg
     3361 ctgtgtacgg cagggctgtt ctggttttcc taccggcttt tcgccctgct ttgctaccgg
     3421 cccagctccg atctgagcat ccacatgacg tgggcgggnn nnnnnnnntt cgtcctcatt
     3481 gccttgctgt tcttccggga ggaaacgctg cggggctggc gggccgtgct ggccgcgctg
     3541 tgctgcgtgc tggtatccag tctgcgcctg gccttctata atcctaccgt ctatttgggg
     3601 gtcggcacgc ccaatacgtg gcacagccct acccagatga tctccatggt gtggatgctg
     3661 ctgtgcgtgc cctacgtggc ccgctgctac gatgacggca tggccctgac cggcacggag
     3721 cactcccggt tgaactggaa gcgtcaggcg cttctgtgtc tgctgctgct gggcagtctc
     3781 gccgcgaagc ccacctttat gcagaccttc cttcccgccg cctgcctgtt ctatctggtg
     3841 caatggaatc gccgccccaa agccacgccc tttgtggtgc agaccctcct gtgggtgctg
     3901 cccgccgtag cgctgatggt gctacaatac ctgtactatt tcggcatcat cgtgccctat
     3961 cagagcggca tggtgctgga aatgagttgg gccaagctgg gcagcgtcct gctggccacg
     4021 ctgctgatcc aggcctttcc cctgtacgcc ctgtgggtct gccgccgtca gaagaaagac
     4081 accctgttct ggctgaccct gtggatggat gtgacgggcg ttgtggaatt tctgatcctt
     4141 ggcgaatccg gccgccgggc cgccgacggc aacttcgcct ggggcatgat gggcgcggcg
     4201 ctcatgctgt gggtggtcat gctgccccgg ttcctccggg atatccggga gcgcggcaag
     4261 ccgtcccccg ctcactgggc ggggttgggc ctgctcctgt ggcatcttgg ttccggcatt
     4321 tattatctgt attatctggt cacgtccggc aatgcgctct gagcgccgcc ctgctgcgtc
     4381 aggccgctga gcgtccgata ttccgccagt ccgctggcaa tggcttctgc gatcttccgc
     4441 tgatattcgg gagacagcag aagcttttct tctgccgaat tgctgatgaa gccgcactcc
     4501 accagcgccg aggggagtcg ggagcgcagc acgtaatagt tccctgccat ggccgtgcgt
     4561 tcctttttcg gcttcagccc ggcgatcaga tgcttctgca atacgccggc caacagccgc
     4621 cctgcgtccc cgccccgctg gtaaaacacc tgcggcccgc tttcgctgcg gctgcggtat
     4681 tcgttcatgt gaatgctcaa aaacacatcc gcctgatttt ccaacgcgat attcacccgc
     4741 tgctgaaggt cttcccgctt gcgggccttg gtggcggtgg ttccttcctc gcaaaggcaa
     4801 atgtcctccc gccgagtcag gatcaccctg gccccctgtg cggccaattc tttttccacc
     4861 gccaacgcga tttccagatt gatgtccttt tcccaccgtc cgctgtcccg ggcacgagcg
     4921 ccgccgtcat agcctccgtg gcctggatcc agcgcgaccg tcatcccggc cagcgcggct
     4981 tctccccgtt ccgtggtctg gacgtccggc agcgcctccg gctgcgtccg caggcacaaa
     5041 catacggcca tgcagctcag cagcagcaac atacatcccc tgaatcgaga ctttttcctc
     5101 tccagcagct ccgctaccgc tctcatccag ccatttccct ccatttttgt ctgaacctat
     5161 gcatattcgt tgcaacatat gaccgaaata tttccatcca tagcattcaa tatacaaaag
     5221 ccatgacttt cccatgacgt tttcctaact ttccacatag ttttccactg aaaaagccct
     5281 gttttgcccg gcaaaaccca ttttatgtgg aaaacttcct gttgaaaacc ctctgtataa
     5341 aagttttcca catacgggaa aattgtgctt tctttgtgga aaagcgtggt ctctgtcgaa
     5401 aaaacatgcc gcgatcagtc gaaagcccca aaataccgtc atttcaggaa gaaatccatg
     5461 gatatcgatc agacgcagga ttgctgcttt acccatgagg aaaactggtt ccgttaccgg
     5521 gtaggggcca tcattctgga ggaaaactcc gttcttgtgg cgaccaacga cgcttgcgac
     5581 ttttgctact c
//
LOCUS       DAWAEA010000046         5532 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2326_length_5532_cov_2.645061, whole genome shotgun sequence.
ACCESSION   DAWAEA010000046 DAWAEA010000000
VERSION     DAWAEA010000046.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5532)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2326_length_5532_cov_2.645061"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4583..4611
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1054 a         1781 c         1690 g          978 t
ORIGIN      
        1 gggctgctcc ttccgggtga cgcgcaccag cgccttttcg ctgtccttgg tgaggaaagc
       61 ccgcagcacc gcgtgatcgg gctggggcgt tcccctggtc aggcagagat agcgcttgtg
      121 gatgcgccgc tgccggaagg cttcttccat agcggcctgc gcgtcctcgt cccggcacag
      181 cagcagaagg ccgtccgtct ggttgtccaa acggtggcac aaaagatgtt cccgcgcgtt
      241 cgattcctgc cggcgaagct gccgcgccac caactcggtg atggtctgtc cgctctttgc
      301 gtccttctgg cagctgatcc ccgcgggctt gcgaaccacc agcacccggc tgtcctcata
      361 caaaatggga atttccggct cccgaatttc ctcgccgggg gtatagagcc gcacctccgc
      421 gccgggaatc agcacggcct gccgatcgac tcgcttgccg ttcagcttca cgtcccgccg
      481 atccagcgcc tcccgaacgg catactccgg cagctggggc agcatccgcc gcagccaggg
      541 aaaaagccgt cccggagcga tgtcctccgg gatggtcaag gtatacagtt tcatcttaat
      601 gctccagcgc cgctttcagg cccagccagc gcggcgttcc ttcgtacaga cggcgcagcg
      661 cgtcccgcat ttcccgggtg ggcagcaccg tgacggattc ggccagcacg ctctccattt
      721 cggaaaggga ggcgttctgc ttggccagca tccgcaagtc ctcggcaata tcgttcacgt
      781 ccagctccgg ccgcagcgcg ccccgcaggg cggcacacat ggcttcatgc aggggcactt
      841 cctccggcag gatgccgttc atgcccccgg ctaccatctg ctggctcatt tcaaagctgt
      901 ccatggcgcc cacctgctgg cgcttcacca gctgataggg gttcgccaga ccggggtgca
      961 gcaggatcat gccgcccgcc ccgtcggtga tgtaatcaaa ggaggtctgc agatagcgcc
     1021 gggcgatctc ccgggccatg gggtcgccgc gctgcatcac gccgcccaga aagaattcca
     1081 tggcctgtgc cgcgtgcagc agaccggtca gatacaaaag tccatgtacc gtcgcgtcat
     1141 agcgccacag ccgttcccga tccatagccg cgccgtcggc gctcatggcg gtcaaaagcg
     1201 gctcctgcag cgcggcgggc agggtcagat accagtcgtc cccttccgcc cgaaagctgc
     1261 accacaagcg gctcaaaagc gcctccgccg cgcccagatc gtcccagtcg gtcagcagca
     1321 gctctccgtc gttgatgagt agcttttcca aaagctgatg ctctcccgcg cccacgaacc
     1381 gggcttccag cggaagccgg gccagcacca tgccccgcag ctcgtccagc gagcagagcc
     1441 gctgctggcc gggccgaaaa tcaaaggaat gctcataaag ggactgcgcg tccagctccg
     1501 tcgcttcagc ggaaaaaagc gccgccgaag gatgggcaaa aagcctgcgt tcgcaggcct
     1561 cgtactgttt ttcccgcatc atgctcatgg tcatttcact cagatcggta tgaaccgtgg
     1621 attcccgcag catggctttc cccccttttc gttgcatcta tccattataa cgggcccccg
     1681 tgcggtgtca aggttctttc cccagccaat tcataggaat ggcggggaaa atccccttat
     1741 tccgggaagg tcaccgtcac tgtgccgtcc gcttcgcttt cggcggcggt catccccagc
     1801 agaccttcga gggtctcggc gggcagatag agccattcgc cgtaggcgcg gatgtcccgc
     1861 ggccggagca gctgacgcac cccgttcagg gtgatttcca gattgatggg cgttcccgcc
     1921 tgattgaggc cgtaggacag ccggtacagg ctgccgccca tggcgaaggc atagccgggg
     1981 ttatccacgc tgtccgaggg cagaatggtg gctccgtggg ccagcagcac ttcccgcagg
     2041 ggcaggtaca atgcgccgct ttcgctgcgg cagggcggaa tgaccgcttt gtcttccccg
     2101 gtcaccgtct gcccgtccac gacgattgtc ccattctcta taggttccag cacggacagc
     2161 ggcgcttcgg tcgccgccat gggcacggga gtgtccgtgg gaatcggcga cggcgaaggg
     2221 gtaggcgcag gggtatcggt aggcgtggga gaaggctccg gcgtatccgt aggcgcgggg
     2281 gtggcatagg cagcccaata atccgcccgg cgcactttgc cgctgtcata aagcacagac
     2341 agggtattcc ggcccgcgat accgtccgcc gtcaggccgt ggctgtattg aaagcgttcc
     2401 actgcccgcc gggtctgctg gccgaaacgg ccgtccagat cgccttcata atagccgtac
     2461 tcggccagcg cctgctgcag gcgcagcacc tcgtcgccct tatcgcccgt ctggatggtg
     2521 tggtaatccg ggttgacgga tacctccacc gtaggcacgg cggtggggcg gggcgtaggc
     2581 tccgccgtgg ccgtcaccgc cggggtgggc gtgacaaccg tagccatata agcttccgcg
     2641 tccggcatac tctgcacgga ggacgcgtac accatcgtca tatagacaag cagcgttttc
     2701 agcagatcca tcgtttgacc tcccggtttg atttagtccc gaagggcgcg ccgcccgtgt
     2761 ctgcgccggg caaagcgctt cagcggtacc cggcagaaaa ccacgccgtc cgcccgaaga
     2821 gagcgcagcg cggcgccgag ctcccggggc gtatagcgtc cgtcctgcgc cacggcgacc
     2881 gtcatatcgg ccaccgcgcc cagcatcgcc gcgtcgctgg ctgtccggca gggcggcgcg
     2941 ttcaaaatca cataatcgaa ggattgggcc gcgtccgtca ggaaggcggc aaagccggga
     3001 cgggcggcaa tccggctgag gaatcccggc tcatggcaca cgtccatgaa caaaagtcga
     3061 ggaatgggcg tagacaaaag cgccgtgcgc agcgcgtcct cccgctgaag acagtccatc
     3121 acgtccatcg tgccctgcac gcccagccat ttccgcaggg cgggggcata ggtatccatt
     3181 tccatcagca gcacttcgaa gccctgcgcc gccagttcgg aggctgtcag cagggaaaga
     3241 gtggcgctgt cctcgtcctc cagaccggcc atcaggatgg aacagccggt atgctgcggg
     3301 cggttacgca gctgcagaac cagctcccgc acgttttccc gggtcagctc gtccatgccg
     3361 tcgtacagcg tggacgcctt gccccgccta cgttcccgca cccagcggcg caggcagtcg
     3421 tcgtattccg ctacgcctgc cagacagggc agctgggcgg gcggttccag tggatcccac
     3481 tgaacccgtt cccggggagc agggatcagc agcgccagca acgaaaacaa cgcaaaagaa
     3541 cccagaaacg ctgccgcgat cttgacgggg cggttaggcg caaaaggcac ttccgatacc
     3601 tcggcccgcg acaccagatg gacctccgcc gcgcccatcc ggccggacag ttcttccagc
     3661 aaagcgtccg ccacggcgtt ggcaaggccc gccgtcacag cagggtcttc tcctacggct
     3721 tccaccttca gcatatggcc gccgtctgcc tgacggatgt acagccgcgt tttgccatcg
     3781 cttcgcgtgc tttgcagcac cttttgctga aattcagggg tattgaccag caggtcgcag
     3841 tcccggcaca gcatccgggc caactccgca gcgtccgcat cggcgcgctg tccggcgggc
     3901 agcgcgtaca ccgtacatgc cgcacgatag cgctccggct cccaacggac agtatagacg
     3961 gccatggcca gcgttaccgc cgcgcagaag aaggcgatca ccggcaaagc ccgcagccat
     4021 tttcgacgaa gccgtttcac gcacagcacc tcttttcccc ttccaaaaat acaagacaag
     4081 acggctatag aatagcacag aaaagcgggg ttttgcaagt tctgcaggac ttcggcacgg
     4141 attccgggtt tctttccctg tttttacatt tttaacagaa tgtaacaaaa tcattgagga
     4201 aagaaaacgt tttgttcaga aaaaaatggg agaaagatgc aaggattgga cggttcgttt
     4261 cgtttcaata taggtacgaa ggatgcatga agcccggagc gcgccgaact ccggggcaag
     4321 gatggcgagg aacaggaatg acgaactttg aggagctgta cgagaaatac gcaaacgacg
     4381 ttttgcgcgt cagctatttt tacctggggg accgccagcg agccgaggac gtctgtcagg
     4441 acgtctttgt caggctgatg accaacgcgc ccgacctgca ggagggccac gaaaaagcgt
     4501 ggttgctgaa ggtagcgctg aaccgctgcc gggacttatg gcgcggcgcg tggctcaaac
     4561 gggtcacgct gggaagcccg gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngagctggtg
     4621 cccgccccgg acgaccacca gcgccgggag gacgaggagg ccatgatgtc ggccgtccac
     4681 gccctgcccc ccaccttccg ggaaaccata ctgctttact actatcaggg ctacggcatc
     4741 ggcgagatcg ccaagatgct ggaactgccc gaaggaacca tctccagccg tctgtctcgc
     4801 ggacggaaaa agctggagga actcctgaaa gagagaggga tcgagccatg aagaaactgg
     4861 aacaactgcc tgagattgcg gcgcgtcagt taggcgggtt ggaagccacc ccggccctgc
     4921 tggctaaaat caagctgaaa gcggcggaag aacgcactcc ccgcaagccc gcgtggaagt
     4981 ctgtcgccgc gctggccacg gcctttgtgc tgctggcggt gggcgcggta tcgtggcaat
     5041 tccttcccaa agaaacgtcc tccggcgacg gcgagctgat gcacacccgt tccgccggcg
     5101 gggagatccg ggagacggag gtgcccgtga ccgccgacct gccctcgggc agcatcacac
     5161 tgggcgacgg ccggaagggg agctctgatt cgctctttgc cagtgcggac aacggcgttt
     5221 tccccatgct gatcgtcaac ggcgctactt atcggatgct cacctacccg gacggcattt
     5281 ccgacgggct gctgggcagc tcgctgggca cggtgaacga gtacaacgtg gaacccgcgc
     5341 tgggttccgg cagcatcgtc agcaactcgg ccagtcaggg cgaaacggtc tacgagatcg
     5401 acggtttcag cggcgctctg gccgcggcca aggtcaacgg cagcacccgc gttttccagc
     5461 gggtcagcta tgcgggcacg gccaccatcg gcagcgaagg tctgaacgac acgctctgct
     5521 cggcctctca gg
//
LOCUS       DAWAEA010000047         5443 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2369_length_5443_cov_2.014291, whole genome shotgun sequence.
ACCESSION   DAWAEA010000047 DAWAEA010000000
VERSION     DAWAEA010000047.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5443)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2369_length_5443_cov_2.014291"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1096 a         1623 c         1765 g          959 t
ORIGIN      
        1 ccggccttct gaccggcggc gatattctgg cagcgctgac cccggaggtc tgcgaaggca
       61 tgaaggagat catgctgtgc agcgtgaccc ttcgccacga acaggatcgt tttctggatg
      121 atatgaccat cgacgagttc cgcaggcggg cccccctgcc tgtgcggctg gtggactgcg
      181 acggccagtc gctctacgac ggcctgcatg gccgaggtct ggactgaacc atcgggaata
      241 aggaggaaac catggccaaa cccttagttg ccattgttgg caggcccaat gtgggcaaat
      301 cgactttctt caataaaatc gccggacggc gcatctccat cgtggaggat gtgcccggcg
      361 tgacccgcga ccgcatctac tgcgacgtgg agtggatggg ccgtcatttc agcatggtgg
      421 ataccggcgg tctggatatg cgcagcgagg acgtgctgct ttcccagatg cggcggcagg
      481 cggagatcgc catggaaacg gcagacgtga tctgcttttt ctgcgatggc cgtaccggcc
      541 tgaccccgga tgacgaggac atctgtcatt acctgcgcaa gactcacaag cccattttgc
      601 tggtggtcaa taagatggac tacgcgggcc tgaacgacca gctgtacgaa tactacaacc
      661 ttggtctggg cgatcccatc gccatcagcg ccaccaacat gctggggctg ggcgacctgc
      721 tggaggccat cgtccgccat ctgcccccgg ctcagcccga cgaggaagaa gaggaagaac
      781 acgtgattca gctggcgctg gtgggccgcc ccaacgtggg caagagctcc ctgtccaaca
      841 agctgctggg gcaggagcgc accatggtca gcgatattcc cggcaccacc cgggacgcaa
      901 tcgataccct ctttaccgat acggacggca cccgcttcaa catcattgat accgccggaa
      961 tgcgcaagaa aaaggcggtg gaggatgaaa cactggaacg ctacagcgtg ctgcgctcca
     1021 tcgccgccat cgaccgctgc gacgtggccc tgctgctgat cgacgcccag accggcgtga
     1081 ccgaacagga taccaaggtg gccgggctga tcctcaactc tggcaaggcg gtcatcgtgg
     1141 tggtcaacaa gtgggacgct atcgagaagg acacccacac catggaggcc atgcgcaaga
     1201 agatcctctc cgatctgaag ttcatgagct acgcgccggt gctgttcctc tccgccctga
     1261 ccggccagcg ggtgaatact gtgctggacg cggtgaaagc cgcctatgct cagtactcca
     1321 aacgggtgcc caccggcgtt ctgaatgaag cgctggccga tgcgcagatc agcctgcagc
     1381 cgcccgtctc cggcggccgc cgcttgaaga tctactacgc tacccagcag agcgtctgcc
     1441 cgcctacctt cgttctgttc atcaacaacg aggaactgat gcacttcgcc tatcagcgct
     1501 atctggaaaa ccacttccgc aagtgcttcg gcttcgaggg tacgcccatc cgcttcatcc
     1561 tgcgggaaaa gaagcaggac ggcaagaact gacgaaacgt ctgaaaaacg catacatgct
     1621 ttttcgtcaa accgcgcaca ctgtccccaa agaaaggatg ggatggtatg cgcggttttc
     1681 gcacggatct ggccatggaa agcgtggggg aggagagcgg acggctggag ggcgtcagca
     1741 tgagcgccca ccagatcggc ggcgtgaagc ggacgcggat cgtgatccag tcccgcaggg
     1801 cggcggaggt gctgcagcgg cccgtgggcg aatacatcac gctggaatgc ccggaactgt
     1861 cccgatgcgg tcagccggag cggccaatgc tcatccgcct gatcgcacag gccgtgcggg
     1921 cgctgctgcc caaggagggc gaggtgctgg tggtggggct gggcaaccgc aacgtgaccg
     1981 ccgacgcgct gggcacccgg gtggtggagc ggacgctggt gacccgccat ctgggggaaa
     2041 gccttgcccg ggaaatgcgg ggccgtctgc ggggtgtcag cgccattgcg cccggcgtgc
     2101 tggggctgac cggcatcgaa acggcggagc tgtgccgggg gctgatccag cgggtacgcc
     2161 cggcggcggt cgtcgccatt gacgcgctgg ccgctttcga cagcgggcgc atctgcagca
     2221 ccatccagat caccaacacc ggcattgaac ccggcagcgg cgtgggcaat caccggatgg
     2281 gcctgacgga ggaaacgctg ggcgtgcggg tcattgccgt gggcgtgccc atggtggtgt
     2341 acgcctccac cattgcccgg gatgcaatgg cccggctgat ggaggaatat cacgtggatt
     2401 ccggcgacca ttccgaagcc gccgggcggc tgctgcagca ggtggcggac ggctttctgg
     2461 gcaatatggt ggtcacgccc cgggagatcg acgaaatggt gctcagcgtg gccgatctgc
     2521 tcagtgaggg gctgaaccgg gcgcttcagc cttctctgga cgaagaaacc ctgcatacct
     2581 accttcacgc ctagcatatc cttctgggaa aaaccccgtc aaaggcggtg attcccacgg
     2641 aatctttctg gaaaaaacga atgctttatc tggcggcgct gggcttttgt ctcggcgtgc
     2701 ttctcggaca gctgctgccg ttggcactac cggcagagga gccggcggtt caggcggcct
     2761 tttcttccac agcggagacg gcgcagcctg aacaggagcc ggaaggagaa aacctgtttg
     2821 ccgttccccg gcagaacgat gcagagccgc ctgcggaaac gcccggccct tcttccgcgt
     2881 ccgcaggaaa ggcggacgga tttgtgctgg aagtcctgag tggggcggcg gagacgcaga
     2941 cgaagggccg cattctgatc taccacaccc acacctacga ggcctacgag cagcaaacgg
     3001 acgcgcccta tgaggaaacc acgaaatggc gcacggcgga cgagcgcttc aatatggtgc
     3061 gggtggggga cgagctggcg gcgctattga ccgggctggg ctatgaggtg gagcacgacc
     3121 gcaccgcctt tgaaccgccg gtgctggaca gtgcctacac ccgctccctg accatgctgc
     3181 agcagcgtct tgcggcgggg gagcgttatg atctgatgat cgacctgcat cgggacgctt
     3241 atccaggagg cagtcagggt ggggccaacg ccgtgacggt gggttcagcc aagctggctc
     3301 ggctcatgct gctggtgggc aagggcgagg ggcagacggg ccggggcttt gatgaaaagc
     3361 cggactggga ggccaacctg cgcatcgcgc aggccgtgac ggacgcggtg aacgcccaga
     3421 ccgagggcct ttgccgaaag gtcatgctca aagcgggccg ttttaatcag catgtgcagg
     3481 tgggctgtct gctggtggaa gtgggcaaca accgaaacac gctgggggaa gcgctggctt
     3541 ccatgcccta tctggccgac gcactggacg aagcatggag cagcctgaag ggcaactaaa
     3601 cgttccgact tgaaaaaccc ggcgcaagca ggtataatct tcaaagaatg aacggcgctc
     3661 cgccgggaat ttcggagggg tttaccttgg atcaacgtcc tgtcggcctg ctggacagcg
     3721 ggctgggcgg aatcagcgta ttgggcgagg ccctgcgcca gctgccaaat gaggattatg
     3781 tttattacgg cgataccgcc aacgcgccct atggggacaa aaccccggag gaggtgctgg
     3841 ggctggtgca tcaggcggcg gaacggctga ttgagctgcg ctgcaaagcc atcgtcatcg
     3901 cgtgcaacac ggctaccagc gtttccgccg ggaagctgcg gcaggagctg gcactgccca
     3961 ttatcggcat ggaaccggcc ttgaaacccg cctccctgct gccgggcgag ggcaaaattc
     4021 tggtcatggc cacccgggta acgctggcgc tgcccaagtt tcaggccttg atggctcaat
     4081 acggccggga tgcggtgccc gtgccttgtc ccggcctgat ggaatgcgtg gagcggggcg
     4141 agttggaagg cccgcaggtg acggcgctgc tgcggcagct gctgggcccg tggctttccc
     4201 agccggtgaa ggcggtggtg ctgggctgca cccattatcc attccttcgc aaggccatcg
     4261 ccgccctgtt cccggcgcaa acgaaagcga atctgcccgt gcgggcgaaa aagaggtacg
     4321 ttcatgaaga aagtcatgct gatcttcggc acccggcccg aagccatcaa aatgtgtccg
     4381 ctggtgctgg agatgcgcaa gcatcccgaa ctggaaacgg ttgtctgcgt tacgggtcaa
     4441 caccggcaga tgctggatca aattctggaa tgcttccacg tggagcccaa gtacgacctg
     4501 agcatcatgc agaaaaatca gaccctgttc gatgtgaccg tcaacattct catcagggtc
     4561 aaggaagtat tggaaaacga aaagcctgat gtggtgctgg tgcatggcga cacctccacc
     4621 gcctttgtga cggcgctggc ctgtttttat ttgcagatcc ccgtgggcca tgtggaggcg
     4681 gggctgcgca cctacaatct gtattcgccc ttcccggagg aattcaaccg gcaggcggtt
     4741 gacctgatct cccaatatta tttcgctccc acggagacca gccgtcagaa tcttctgaag
     4801 gaaggcaagc cggacgagaa aattttcgtg accggcaaca cggtcatcga cgcgctggcc
     4861 accacggtgc ggcacgacta tcagcatccc gagatcgagt gggccaaggg cagccgtctg
     4921 ctgctgctga ccgcccaccg ccgggaaaac ctgggcgaac ccatgcggga gatgttcacc
     4981 gccattcgcc gcattgtgga cgagttcccg gacgtaaagg tgatctatcc catgcatctg
     5041 aaccccgtcg tgcgggagat ggcccgcgcc tgctttgcgg gcgaggagcg ggtgcatctg
     5101 atcgagccgt tggacgtgct ggatttccac aacctgatgg cacattccta cctgatcctg
     5161 accgacagcg gcggcattca ggaggaagcc cccgcactcg gcaagccggt gctggtcatg
     5221 cgggatacca ccgagcgccc ggagggcgta gcagcgggca cgctgcggct ggtcggtacg
     5281 gacgagcagc agatctacgc cgccgttcgg gagcttctga ccgaccccgc cgcctaccac
     5341 cagatggagc acgccgtcaa cccctacggc gatggcaaag ccagccaata catttgcgac
     5401 atcctcagca gggggtgacc ccctgcaccc ccgaactgcg cgc
//
LOCUS       DAWAEA010000048         5385 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2407_length_5385_cov_2.338649, whole genome shotgun sequence.
ACCESSION   DAWAEA010000048 DAWAEA010000000
VERSION     DAWAEA010000048.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5385)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2407_length_5385_cov_2.338649"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1295..1304
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2778..2787
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3686..3762
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4542..4551
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          961 a         1726 c         1518 g         1073 t
ORIGIN      
        1 gctgatcctc cgtgccgtca aagccctgcg aacgcagaaa accgtccgtc aggcgctcgt
       61 tgcccgcccg cagcagccga gtgcgggaac cctcggcggc agtcagcacg tccttgagca
      121 cgttttcgta ccagcccacc tgatcctgat agcggtaatg aaagcccccc tgacgatact
      181 tggcgctggc ccggggataa atgctgtcct cgtgctcgtt cacgccgccc atgacctgcg
      241 aggcgtccag ccacacatcc acccgcagcg ggccctcttc ggatgaaaca gtggcggaca
      301 cgatctcggc cgtttcctca taggcgggca tggacagcag ctccagcgac ggggacgctt
      361 ccaccgtccg tccctccacc ttccagcggg aacagccggt aagcagcatc agcgccagca
      421 gacagaccgc cagcttcttt cctcctgcgc agcggggctt tcgcatccgc gccgcctcct
      481 ttcgtatcgt caatcgtctt cttccgtgcg aacccggcag gcttcctcca ccgcctgtaa
      541 atagccgggg ccgttgcggt acataccgtc atacaaacgc atataggccc gataacgccg
      601 gggggcggcg tattccaacg cccggtttcg gcagtctctg gggcttttcg gctgatcgca
      661 cagctcccga acggcctgtg ccagcccgtt ataatccccc atgggcacgg tgcggcccac
      721 ctcgtccgtg acgatctccg gcatggcggt cacatcccag caaagcacct gcgtgccgca
      781 ggccagcgct tccaccagcg tcatgcccat ggtctccgca tggctcaggc tgacgcacac
      841 atccgccgcc gtgtacaggg cgcacaggtc gttcaggttg cccgtgcggg gaatgcccag
      901 caccgatttt tccggcaggg aacgaatctg atattcgtcc agccccaccg ccgccacgca
      961 gtattccggg cccagttttt ccgccagttc cagcaggtct tccagtccct tgcgcggctc
     1021 ccacgtgctg gccacgctca gcaccaaatg ccgtccctcc gcttcgtcca gcccgtaata
     1081 atgggcggcg ttgcgcagga aggcttcgtc cgcgcagggt gcaaacgcgt ctgtatcgat
     1141 gccattgggc agcgcgtaca cgggatattg ccctaaagag gagctttcca cctgcgcttt
     1201 cagccactct gacggcgtgg tcagcaccag atggggcagt ccgctgaaca acgtccgttt
     1261 ttcccgccag tttcgggcgc tctgatcccg gaacnnnnnn nnnnctccgg ggatattccc
     1321 gcaaaaggct gcaatggccg cagccgctct gccagcgcag acagccctcg ccgcaggctt
     1381 ccgaccgccg ccgtctgcct cccccgtcca ccggcgcagc gtcctccccc agcgaaaaat
     1441 aggcgcagtg ccccgtaaac gcccagcagt cgtgcagcgt ccacacggtg ggaaggtcgt
     1501 tttccttcaa ataggcaaac agtgtaggca gatgaagata ggagccgtgc agattgtgca
     1561 gatgaacgat gtccggcttg aactttttca gctgcgccac cagcttttcc gtttcccggc
     1621 ggccaaagaa gccctgacga tcggtcagcc gggaaagcag cgtgtagcct accgtctgcg
     1681 ccgtgctgcc gatgcggtag ccgggcacgt ccgaggggca gctgcccctg gcgtagcaca
     1741 aaagcgcccg gtggccctcc ctcatggcct gacggcatag ctccacggca atgcggccag
     1801 tgctgaaggc ggctgtcacg ttgatgtgaa cgattctcat gagcgggcgc tcccttccgt
     1861 ttccaacttc tgctacctgt tttccttctg agaaaacgct ccgggagcgc cgctcccttt
     1921 tccgggtttg ccgcgccccc gaagcgtttg gactcttagt aattttcggc tttcagcttg
     1981 aaataggcct tcggatgacg gcagaccggg cagacctccg gcgcgctggg gccgatgacg
     2041 atgtggccgc agttggagca ctcccagatg cggtcgccgt cccggctgaa tacaagccct
     2101 tcccgcacgt tcgccagcag cttgcggtag cgctcctcgt gggtcttttc gatggcccct
     2161 acgccctcaa acagccgggc gatttcgtca aaaccctcct gccgggcctc ctccgccatg
     2221 gtggcgtaca tgtccgtcca ttcgtaattt tcgccgttgg ccgcgtcctc cagattgacc
     2281 tcggtggacg gcacctcgcc gccgtgcagc agcttgaacc acagcttggc gtgctccttc
     2341 tcgttggcgg cggtttcctc gaaaatctcc gcgatctgca catatccatc cttgcgggcc
     2401 ttagacgcat agtaggtgta cttgttccgc gcctgacttt ctccggcaaa ggccgtcatc
     2461 agattggctt ccgttttggt tccctgcagt gacttcacga tgttctcctc cttgccgccg
     2521 aaatgcctcc ggctttcctt accttgtatt ctttccaaaa aatgacccgg tgaatccgtt
     2581 ttttcaatag ggtctctgtt ttttccaacc atcagagacc ggcgagccgc gaggcccttc
     2641 gccgagcggc ggtaaaaccc cggccaaccg gctttccctc gagcactccc gcgctctatg
     2701 ggcgcggcag actgtcgccc cccttgattg gccgtcagcg cccgcgaaca gcgcttcgct
     2761 ggttcgcgga aaagtgcnnn nnnnnnngca ctgcccctgc caaggtttgg cagaagccta
     2821 agaccgacca gtggtctgtg tgaagggcgt attggatcag gggatcgacc tgctccgatc
     2881 cgggacgctc ggccatgttg ctgcggagac gcagcccctt ttccaccccc accagatgat
     2941 tgtccaactt gctgtcgtat agataggagt aactcttgac cgagttggac atattgcctt
     3001 cgatggtggt gacggcgtag accccgtctc ccctgtcctc cacgtccacc accgaaccga
     3061 tgtgtacggt tcggtattcg ttgctcatgt ccgcataaat caccagatag cccggctggg
     3121 gtatgcccac aaagcgcccc atggacagga aaccgtcgta ctgattgcct acctgccctt
     3181 cccgaaggaa ctgtacctcc ggtgggtttt ccttgtccac atcgcccttt ttcacaggct
     3241 ccgccccggc ggcgttggcg cagtaaatgg tgaagaccga gcaccagcct acttcattct
     3301 tcttgcgata gtaccacttg gcatacttgt tgtacttggg cagacgggcc ctctgcacct
     3361 ccgtaaactc ccggcgaagc acgtaaagat agttgcggat cagttgaggc atggcgtcgt
     3421 ccgaaacgct gaccttcaag acctgctccc cagcagaggt gcggtacgtc agcgtggtct
     3481 cccccgctga aagcgccgtg accatgccgt cctccgagac ggacagaatg ctttcgtccc
     3541 ccgagatcag ctcgccctcc aaggcgggca atggccgctc tgcctgctca tcatagggga
     3601 tcacctccgt ggatacaaag ggcgcagcca ccgtctcccc ggtgcgaagc tcccattcgc
     3661 cgtccgtcaa agcctgctga gcgggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaaaacgat ctctccctcc
     3781 gtgttttcct tccgattata ccataaactg ccccaaaaca aaatctcctt tgtaatggac
     3841 ccgcgattgt gctatcatgg aaggtgaaag gagaggacat ttcttgaaac tgctcctgca
     3901 tatctgctgt gccccctgct ccattatgtg catcgagacc cttcgccagg agggcattga
     3961 gccggtgggc ttctgggata atcccaacat tcacccctat accgaatacc gcagccggaa
     4021 aaactgtctg gtcgattatg ccaaagacat cgggctggaa ctgatcctgc acggcgatta
     4081 cggtctgcgc ccctttgttc agaccgtggc ggacaacatc gacggccgct gcgtcaactg
     4141 ctaccggatg cggatggagc cgaccgcccg ctatgccgct gaaaacggct ttacccacat
     4201 ctgcaccacg ctgtttgtca gcccgtacca gaatcacgaa ctgctgcggc aggttacgga
     4261 ggaggaagcg gccaaatacg gcgtggagct tctgtaccgg gacttccgtc cgtggttccg
     4321 tcaggggcag gacaaggccc gggagctggg gctatatatg caaaaatact gcggctgcgt
     4381 tttcagcgag gaagaccgct accgcaagcg caaaaaaaag gctgagccga agccggagga
     4441 gggcaagcta tgagcgaccg tctgctgccc tccggccaat ggctgaagga tcgcctgtcc
     4501 acccgctggg tgggcaaaag cgagatcgtt tacgaagaag annnnnnnnn nccctccacc
     4561 aacaccctgc tgaaggagtt ggcccgtcag ggcgcgcccg cgggcaccgt ggcggtgtgc
     4621 gactttcaaa gtgccgggcg cgggcggctt cagcgagtct ggaaagcggc tgcgggagaa
     4681 acgctgcccg tatcgctgct gctcaagccc cgtcttacgc cggagcagat tcccctctgc
     4741 acgctggggg cggcagttgc ggcttcgcag gccatcgaac aggtctgtcc cggcctgaaa
     4801 gcgggcatca agtggcccaa cgacgtggtc atcagcgggc ggaagtgcgt gggcattctc
     4861 tgtgaggggg cttttgaccc ggagggacgg ctctgcgtca tcatgggcgt tggtttcaac
     4921 ggaaatcagc gggagtttcc gccggagcta tccgcgtcgg ccacgtcgct ttttctggaa
     4981 aaacagcgct gcgatcccgc cgccgcgccc gtggtcctac gggagcttct agccgccttt
     5041 ctggaagcgg tggagcgggt catcgaccgc ttggaggcgg gcgggcttcc cgccatcctc
     5101 ccggaatacc gtgcccgttc ggtgacgatc ggctctacgg tacgggtcat tgcgcccacg
     5161 gaggaataca tcggtcaagc ggagggcatg gacgacgcag gagcgctctt agtccgggat
     5221 gcttccggct ctttacgggt ggtcgtctgc ggcgacgtat ccgtccgcgg tctcatgggc
     5281 tacaccccag gggcagtgcc cctggacccc ggaccgcgcc catagggcgc ggggtgctcg
     5341 gagaaaagac agttggccgg ggttttaccg ccgtggcaca acagg
//
LOCUS       DAWAEA010000049         5324 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2442_length_5324_cov_2.005504, whole genome shotgun sequence.
ACCESSION   DAWAEA010000049 DAWAEA010000000
VERSION     DAWAEA010000049.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 5324)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5324)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5324
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2442_length_5324_cov_2.005504"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    629..638
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1130..1139
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1541..1603
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3825..3834
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          999 a         1619 c         1564 g         1049 t
ORIGIN      
        1 cagaactgcc acgcgctgac gtaggcatgg agctttcctc agggcaacca atcagcacgg
       61 cccgcactgc cacgcgctgc atttgccggt tgctgtatct gtggtcgcat ggggaaagaa
      121 acgaacgcgc attcagggcc gcaagccctg ttcccgcact ggaactggca aaagcgagag
      181 aaaatgcgct tatagccgca ctgcccccgc tccctgcggc gcagccccat cacccgtccc
      241 catgcggcac ataaacttcc ttatccgttc tgctccgtcc ctttctcccc ggcaggaact
      301 tccgtctgct tctcttcctc acttttccct aattcctcca gcctatcccg cagctttttg
      361 ggaatgggca ccccgcagcg gcctaaattc tccaagaggg aaagggcttc gttgctgatg
      421 taaaaccaca tgaccgccgt ctggaacatg gcgctgccct gtcctacgta ccagtccagc
      481 agcacgctta accctacgac cagcagaatg accgccttgc gcagaagccc cttccagccc
      541 gcctgactgg acagcctgcc gtgggcggtt ttccggctgc ggcccagcgc cgccgccgaa
      601 gcacagaagc tgggcacgcc gggaatcann nnnnnnnngg cggtacgccc ctgcccgttg
      661 gtcatgccgt gaagaaaacc ggcgatggcc gccccgccgc cggccagaag atcgagggta
      721 tttgcaaaaa aatcccgcat gatccgccac tcctttccgt ctggcaagga tatgacgagg
      781 catgcgggtt tcatgcgccg ggcttattcg ggacgatcca ccacgggatc gtcgccccgg
      841 tcgttcaggt agcggtcgat gatgaaacgg ggacgctgct tggtttcgtt gtaaattttg
      901 ccgatgtatt cgccgattac acccagcgac aaaagctgaa tgccgccgat catccagatg
      961 gaggccaatg tgctggtcca cccggccacg gtgtgcccgg cgatccacga gatcagcgtg
     1021 tacagcagca tcaccagcga aatgaggaaa accacgatgc ctgcattggt gatcagccga
     1081 aggggcttga cggagaagct ggtgatgccg tcgatggcaa aggccagcan nnnnnnnnng
     1141 gaagcctacc atgggcgcca gtccccgcgg gaacagattg acctccttga actcggccag
     1201 cgcgttcagg gcccggttgc tcatcagccg gtagtccgcg tggttgaaga cgatgtccac
     1261 ccccatgccg ctcatcagcc ggtagaacat cagcgcggtc tcccgcttga agaagctgtc
     1321 cttttcccgt ttgctgcgca caccgtagac gatttcgcag ccctcgcggt gcttctcgat
     1381 gaacttgtcc atggcgtcca tgtcgtcctg cagatccgca tccatggaaa tgcagatgtc
     1441 gcagcggttc cgggccgtca tcagcccggc cagcagcgcg ttctggtggc cccggttccg
     1501 gctcagcttc acaccctcga aaagcgggtt ttccttgtgc nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttttgat caccagccgc
     1621 cgggacgttt ccggcagcac ttcttcttca ttataacagg gaatgacgac catcagtttt
     1681 tccgccatgg tgaagcctcc ttgtctggct aaaaattatt gcacttcaaa gggttccgat
     1741 tccacggaaa gggtctgatc cttccagtgg atctgggccc ggacgcggta cgtgcccgcc
     1801 ggggcggggg aaccatcccg cagactgccg tcccagtaaa gggtagtgcc cgccagtccc
     1861 atgggcctgg tagagcggcg gtggcacaga cgctccacca cctgcccctc ctcatcgaga
     1921 atggctacgc tcaaagaaca gggagaatgg tgccccacaa ataccgccag ttcccgcccg
     1981 gtttccgggg cgaaggaacc gctgatctcc acggtggggg cggacggttc ttcctcgggt
     2041 aatacgcaaa gcacccggcc cgcgtggacg cgcagcttgc cccggtcgat gctgaccagc
     2101 tgtaccagcg cgtagccgta ggcgtcgtcg gccagctcgt ccagccgaag ggtacgctgc
     2161 ttccacccgg cggagaccgc gccttcctga tcgccgaagg cggagaagcg ctcggagtca
     2221 tcgtagatca gctgggcgtt ttcaaactcc caccggccct ctcggcggta taccagctga
     2281 taggccaccc gcaccgggcg catagcaatg tattcgatgg tgatctcccg gctgtcccga
     2341 tccaggatca ggtcggaaaa ggtcacgtcc atgatggggg agcgggaaag gcccgtgggt
     2401 agcggctccg acaggtcgta acggaagacc acaaaatcgt cgttctgggg atagacctgg
     2461 ggcgtggagg cggcatgatt cagcagcagg tagtcgtaaa gctccttcag ggtgatgctg
     2521 ccgtcgtggt tctggtcggc cgggtagccg caggcggcgc tgagcgcgtc gcacagggcc
     2581 tgcgtgaaat aaaacgcccc ctgccggtcg ttctgcggtt cgtcctctcc ggcgctccag
     2641 taccagcttt cctcccgggc gccgctggag gcgatcacct tgaaatcgtc ccccagaaag
     2701 cagggcggct cgtcccattc ggtcatgccc ttgccgatga aagcgccgct gttgcaagcg
     2761 tccagaatga gcaccttcgt gcccgaaatg ccgtcgaagg cagcttccag cgctttgggg
     2821 gtcagacggt tctccgtctg gccgtcgctt agaagcaagg caggttccac accgttttcc
     2881 gggtcgtata ggccgtgggt gctgatatac aggtagctga cgtccccggc ctccgcaccg
     2941 ccgaaaaccc gccggatcag ctctgtcagc tgctcgacgg aagaaacggg ttcggcgggg
     3001 accatgatct tttccagcgg cgtcgccgcc gattggaaca tttcctgcat ggcatagacg
     3061 ttgttggtgg agctggggta ggcgctgggc tgggtgacaa aatcgtccac gccgatcagc
     3121 agcgcccgct cccggcgaac ggaggcttcc tcgcctcgga cggcaggaaa aggccaaagc
     3181 gccgcaaggc agaaaagcgc cagccaaaaa gcgaatcttc tgcgaagcgc caagcgtctc
     3241 cctcctcccg tctcatggat gcacaacgcc tattgtacac tattttcggc gattcgtaca
     3301 gacggggaag gacggagaaa tctggttatt cctccaccag ccagttgtcc gggtcgtccc
     3361 agagctgctg gcgcaggagc cgggcaatga gctgctgggt ctcccggtcg gccagcccgt
     3421 tgacggcatg gccgggcaga aattcctgct gcagcgccat cactgccgcc cgagtgtggg
     3481 agccgtaagc gccggtcagg tgcagctcgt ccagaaagcc cagctgataa aggttttgct
     3541 gaagggccaa caccttgtcg ccctcgttgc cggagcgcat ggtggtgtct accaaatctt
     3601 cgcacaggcc gaagcccagc acctgactgt tgtccagcgg gtagaccttc cgctgcaccc
     3661 ggtcaggatt gttgccctcg atgacgtgca ggtacacatt gccttccgca tcccgggagc
     3721 agtactcgat cagcgccacg tggtcggtat ccccggcggg gttgtagctg aaaaacacca
     3781 gatcgccgga gcgggggatg aactcatttt tgcttaaatt atgannnnnn nnnnagccac
     3841 tgcgcgcccc agtccgggca gacacccttg cggtagacga aacggccccg cttgataaac
     3901 cagttccggc ctgtgttctg gccggaatag ttgggataga tcacgttgag caggtcttcg
     3961 cccagacttt gctgggcctg atccacgcac cagcagatga actcagcgca ccactccgca
     4021 taggggtcgc cggaccactc gccgtatttg gtgtagttct gtgccccctc ggcgtagccc
     4081 agctcgcccc gggccacgtc cagaagccgc tggacaaagg cgggacgctc cccttcgggg
     4141 atgggagtat ccgtttcctc cccgagggcc gcgccgcccg ccgaaaccag cagacagcag
     4201 acggtcagcg ccgccgcaag ccgtttgatc cacatgatgt cgttcccctt tccggcccgt
     4261 cagcggccaa aggcctcaat gccccgcagc agctcaaatt cccgaatgtt gcgcacggtg
     4321 gtgtacattt tccgggtcag gacggagccg gtgcggtaga acaggcagat caggggcaca
     4381 tcgttggcaa actgctgctg aatggccatg gaggcatagg cgaaatccgt ctgattttcg
     4441 caggtacgca gctggtcgat cagatcggtc atatcgctgc tgacgtagcg catgtagttg
     4501 cctgtgttgc ccttgcgcag catgaagccg taatcgggca ccacatccat ctggaaggcg
     4561 cacagggcca gctcgtaagc gccgctctgg agcttgctct gcatgtcctg atagctgacg
     4621 gcggtggtct tgctgatgcc gatgcccact tccgccagca tttcttcgat caggttggca
     4681 gtctcgtagc gcacgtcgtt gtccgggtcc tcataggtga tgagctgaag gtacaggtgc
     4741 ttcacgtcgt tgcccacgat cttgtccagc gggccttcgt tgtccagatc ggtccagccg
     4801 tcctcttcca gcagctggcg ggccatgtcc gggttgtaaa cgaagctgct ttccaaactt
     4861 tcgtcgtaca gccagctgtt ggagggcacc atggcgtcgg aatccagcgt catgcccatg
     4921 tacacgttgt tggaaatctg gttgacgttg atggcatatc ggatggcctg ccgcaccttc
     4981 aggctttcca tggggaaggt ctgatgattc atcagcaggc attccatctg ccgggtattg
     5041 taggcgatgg acagggagct aatgccgctt ttgtactggg cggcggctac tgagcgggta
     5101 aaggccgtgt ctacccggcc gtattcgtag gcggtgatga gttccttatt gctggagaaa
     5161 aatgaagcca tgatctgctg gacctggggc tgagtctgcc accagttggc gttggcgtcc
     5221 agctgcatat agctgcccgg ctcaaaggcg ctgatgacat aagggccgct gcccagcgga
     5281 ttggcctgct ccacctgtgc cgcgggcact acggggaagg tcat
//
LOCUS       DAWAEA010000050         5218 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2508_length_5218_cov_2.537672, whole genome shotgun sequence.
ACCESSION   DAWAEA010000050 DAWAEA010000000
VERSION     DAWAEA010000050.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5218)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2508_length_5218_cov_2.537672"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    536..632
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4480..4489
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1085 a         1610 c         1547 g          869 t
ORIGIN      
        1 caagcagctg gacgaggtgg cctatgtgcg cttcgcgtcc gtgtaccggc agttcaccga
       61 cgtgtccagc tttatggacg agctgaacca gctggttcgg gaaagcaagg cccttcggga
      121 aaaagaataa gcttcgcgcc ccgcagtttt caatctgcgg ggcgtttttt tgtccaattt
      181 ggctgtttcc agtccttcca gccttttctg tcgaaaaacg gcagggtttc gggaaaaaac
      241 atcgaaatta gaagtcggga acatttcccg aaatcgcaat tttcattcat caaaaggaga
      301 ctgatgcgca tgaatcggat cctggaacgg gaggcgctca acccgacggt cacacggctg
      361 aaggtggagg ctccgctcat cccccgcaag gcggaacccg ggcagttcgt cattctgcgg
      421 acggacgagg acggcgagcg gatcccgctg accgttgcgg attttgaccg ggaggaaggc
      481 agcgtcaccg tgatctttca ggtggtgggc gcaacgaccg aaaagctgaa tcaccnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcgatatg cttcacgact tcgtagggcc
      661 gctgggccgg gccacccgca ccgagggcct gaaaaaagtg gccgtggtgg gcggcggcgt
      721 tggctgtgcc atcgcgtacc ccgtgaccaa aaaactgcac gagcagggct gcgaggtgca
      781 cgccatcgtg ggcttccgca atcaggatct ggtcattctg gaaaaggaat tccgtcaggc
      841 cagcagccgc ttcgtgccca tgagcgacga cggctcctgg ggtgaaaagg gccttgtgac
      901 cgacgctctg cgcaagctga tcgaaagcgg cgaacagtac gacgaggtca tcaccatcgg
      961 gcccctcatc atgatgaaat tcgtctgcaa gctgacgaag gaatttggcg taaagaccgt
     1021 ggccagcatg aaccccatca tgattgacgg cacgggcatg tgcggcggct gccgcctgac
     1081 cgtgggcggc aaaatgcgct tcgcctgcgt ggacggcccg gagttcgacg gccacgagat
     1141 cgacttcgac gaagccatgg cccgcaacgc cgcctaccgg gacttcgagc gtcatgccta
     1201 cgaagaaacc tgcaaccttt tcaagggggc gaagaccgat gcataatccc tccaagcaga
     1261 aaacgcccat gccggtccag gagccggagg tccgcagccg caactttgac gaggtagcgc
     1321 tgggctacac cgaggaacag gccgtgtcgg aggcgcagcg ctgcctgaac tgccgcaaca
     1381 agccctgcgt gggcggctgt ccggtcagta tcaacattcc cgcctttatc cacgaggtgg
     1441 ccgaacggaa tttcgagggc gcgtatgaga tcctttcccg ctccacggcg ctgcccgcgg
     1501 tctgcggccg tgtctgcccg caggaaagcc agtgcgaaag ccgctgcgtc tgcggcatca
     1561 agggcgagcc ggtgggcatc ggccggttgg agcgcttcgt ggccgactgg cacagcgcca
     1621 acgccacgga aaaacccgtc cgtcccgcgc ccaacggcca caaggtcgct gtgatcggct
     1681 ccggcccctc cggcctgagc tgcgcgggcg agctggcgcg gaaagggtat gcggtcacgg
     1741 tgttcgaggc cctgcatctg gccggcggcg tgctggtgta cggcattccc gagttccgcc
     1801 tgcccaagcg catcgtgcgc cgggagatcg agaacctgca ggcgctgggc gtggatgtgg
     1861 agaccaacgt ggtcatcggc aagacgctga ccattgacga gctgcaaaag gaatacggct
     1921 tcgaggccgt attcatcggc agcggcgcgg gcctgcccaa gttcatgggc atccccggcg
     1981 aaagctggaa gggcgtatac tccgccaacg aatttctgac ccgcatcaat ctcatgaagg
     2041 cctaccagcc cggcagcgcc acccccgtgc agcacggcaa aaaggtagcc gtggtgggcg
     2101 gcggcaatgt agccatggac gcggcccgct gcgcccgccg tctgggcgcg gaagtcacca
     2161 tcgtctaccg ccgcaccgag gctgagctgc ccgcccgcaa ggaagaggtg gagcacgcca
     2221 aggaagaagg catccagttc cgcttcctga ccaatccgtt ggagattctg gcggacgatc
     2281 agggctgggt gcggagcatc cgctgtcagg aaatggcgct gggcgagccg gacgcttccg
     2341 gccgccgccg tcccgtgccg gtggagggca gcgagatgga gctgcccatg gactgcgtca
     2401 tcatggcgct gggcacctcc cccaacccgc tcatcaagtc caccacagag ggtctggaaa
     2461 cccagaaatg gggcggcatc gtggcggacg cggacacggg gctgacctcc cggccgggcg
     2521 tatacgccgg cggcgacgcg gtttcgggcg cggccacggt gatcctggcc atgggcgcgg
     2581 gcaagactgc cgcctcggcc atcgaccggc agctgatggg ctgagcttct tcgatcctcc
     2641 gagaaaaacc gggcggaagg gggccaaaaa ttttcctttc gccccggcat ttcccaaggg
     2701 aaagttcgaa gggctgaata acgccgcatc cattgaaaac actggtgttt ttcaatcgtt
     2761 catatcctta ttgataaggg gtatatataa aaataattat tgcaaaaaaa cacgatttgc
     2821 aggaaaataa gacgaatcac gaaggaaggg gttattttct atgaacaaaa tcacgatcat
     2881 cggagcgggc aaagtcggtt ccaccatcgc gtacacgctg tccgtcatga atctggccaa
     2941 ggacatcgtc atgatcgaca tcaaccacga actggcgctg ggcgaagcgc tggacatccg
     3001 gcagggcacg ccgttctcca gcaactgcag cgtttatgcc ggcgactatc cggacgccgc
     3061 caactccaac atcgtggtca tcacctccgg cgtggcccgg aagcccggcc agagccgtct
     3121 ggagctggcc cagaccaacg tgaacatcat gaagtccatc atgcctcagg tgaccaagta
     3181 tgcgcctgac gccatgtaca tcatcgtcag caaccccgtc gacatcctga cctatcagtg
     3241 gtgcaagcgc tccggcctgc cggagaacca catcatcggt tccggcacca ttctggacac
     3301 cgcccgtctg aaggcccgcc tgtccgagta ctacaacatc agccagagca acgtgcatgc
     3361 ctacgtgttc ggcgagcacg gcgactcctc tttcgtgccg tggagtctgg ccaacatctc
     3421 caacatcccc gtcaccgact tccgcaacaa ggtggaaggc gacatgtcca tggctcctga
     3481 gctgaacacc gccgatgtgg agcagtacat ccgcaagtcc ggcggacggg tcatcgcccg
     3541 caaaggcgct accttctacg ccgtggccgt gtccgtgtgc cacatctgca agtgcctgct
     3601 ggacggcatg gacacgaccc tgaccgtctc caccatgatg cacggcgaat acggcatcga
     3661 ggacgtgtgc ctcagcgtgc tgaacaccgt gggctgggaa ggcgtgcact ccaagctgct
     3721 ggctcccctc accgaggaag agaccggcct gctgcgccac agcgcggacg tgctcaagga
     3781 cgttatcagc aatctggaca tctgattttc caagccgcaa ggaggttttt cccatggaat
     3841 accgcatcga acatgactct atgggcgaga tgaaggttcc cgccgaccgg ctttgggccg
     3901 cccagaccca gcgcagtcac gagaacttcg aaatcggcgt cggcatcgag accatgcccg
     3961 ccgagatcat ccacgccttc ggcatcctga agaaggccgc cgcgctggcc aatcaccagc
     4021 tcaagcctga aaagatgacc gatgaaaaga ccgacgtcat cacccgcgcc tgcgacgagg
     4081 tgatttccgg ccagctgaac ggccacttcc cgttggtggt gtggcagacc ggttccggca
     4141 cccagtccaa catgaacgcc aacgaagtgg tggctaaccg cgccaaccaa ctggcgggcc
     4201 agaagctggt gcaccccaac gacgacgtga acatgagcca gtcctccaac gacaccttcc
     4261 ccacggcgat gcacatctcc gcggtgatcg ccatcgagga taagctgctg cctgccatcg
     4321 agcatctggt ggccaccttc aagaagctgg aagcggagaa cgagggcatc gtgaagtccg
     4381 gccgcaccca tctgcaggat gcgaccccca tcaagttcag ccaggagatc agcggctggc
     4441 gttcctctct ggagcgggac gcggagctgc tgaagctggn nnnnnnnnnt ccgggcctga
     4501 aggagctggc gctgggcggc actgccgtcg gcaccggcct gaacgccccc aagggcttcg
     4561 gcgaagccgt ggccgcgcag gtagcgcagc tgaccggcaa ggatttcatc accgccccca
     4621 acaagttcca cgcgctgacc tccaaggatg agctggtgtt cgctcacggc gccatcaagg
     4681 ctaccgcctg cgacatgatg aagatcgcca acgacgtgcg ctggctggct tccggccccc
     4741 gggacggtct gggcgagatc cacattcccg agaacgagcc cggctcctcc atcatgcccg
     4801 gcaaggtgaa ccccacccag tgcgaggccg tcaccatggt ggccgtacag gtcatgggca
     4861 acgatgtggc cgtgggcatg gccgcttctc agggcaactt cgagctgaac gtgttcatgc
     4921 ccgttgccgc ctacaacttc ctgcagtccg cccggctgct ggcggaagcc atcgtttcct
     4981 tcaccgacaa gtgcgccgtg ggcatcaccg ccaacaagga caagatgtac cacaacctgc
     5041 acaactccct catgctggtc acggcgctga acccctacat cggttacgaa aacgccgcca
     5101 agaccgccaa gaaggcctac aaggagaaca tctccctcaa ggaagcctgc gtggcccttg
     5161 gcttcctgac cgccgagaag ttcgacgagg tcttccaccc cgagcaaatg gtctaacc
//
LOCUS       DAWAEA010000051         5041 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2615_length_5041_cov_2.350983, whole genome shotgun sequence.
ACCESSION   DAWAEA010000051 DAWAEA010000000
VERSION     DAWAEA010000051.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5041)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2615_length_5041_cov_2.350983"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1932..1941
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1048 a         1654 c         1477 g          852 t
ORIGIN      
        1 gccccatggc ggcggtcatg cgcttgatgc ggtatttgat ggtgttttcc gtggtgtgca
       61 gggattcggt catgtcgaaa tagcgtgctc cccgcaaaag ctcccgaagg atgtccaggt
      121 ccagactgtc catcccggcc agcagttcct tgaaacggaa gacctccccc acatgggggt
      181 cctgatagaa atcgccgggg aactccgacg ctggttccgc cggcagcgtg tccgccatgc
      241 cgggttcatc cgccgcttcc aaccggcagg gtacccagac gcagcggcga atgtccggct
      301 ccttttccag cttgcggcat agacgcacgg cctgtgcccc gcaggcgaat aaatccattc
      361 ggaacagaga aatttcttct tcccggggaa gtgcctgttc accgaaggcg tacagccggg
      421 ggccgccgcc gggcagcaga cggcgcagcg tcatggccgc cacgtcgttg gcgcagagca
      481 ccgcatccag tttgtccgcg tcccgtgcca gccgctgggc ggcttcgatc agattgccgc
      541 tgttttcgta aattttaccc tcagggcatt ggcgcagaaa ggcttcccgt ttcacctcgt
      601 ccgccgctga ggacggatgc acggcaaaca gcgcccgtct ttccggggta cgggcggcaa
      661 agatggccgc ggcttcctcg taatccggcg cggcgaaccc gcagcgaacg ccctgcggcg
      721 gtacgcccgc gatcaggcag ggaaggttcc gggccgccag ccgggccaga cggcttttta
      781 accagcctac ggcgctgccc accagcagaa cgggttcctc ggcggcttcc gcctcttccg
      841 tcagcgtttg cagctcccag ccccgttttc gggcctcctc ccggatgccc gccagatagg
      901 cctgcgccca gagattggcc ccgtaggaag gttcgatcag cagttggatc attctttcgc
      961 cccgtctcga ttcgttggat gtaaataaaa tattatatcg ttcttttatc tgaaatgtca
     1021 agccaaaaat gcagccgaaa gggcggcgca tcttgtgttg aaacctgccc cgcagacagg
     1081 tacaatcagc ctatccaaag gaatttcccc caaaaacgaa aggaaggttt gccgatggac
     1141 gttattctca aacatgaacc tatgaccatc ccgacctatg agccttatgc cgccaatacc
     1201 ctgcccttct ttctggaaaa gaaggcctat cagggcgcca cgggccgcat ttatcccgta
     1261 ccctataccg accgtctgtc caacaagccg gtggataagg attacgattc ctacacgctg
     1321 gaaaacgagc acgtaaaggt cgtgctgctg ccggaactgg gcggcaagat ccacggcgcg
     1381 gtggataagc acaacaacta cgaattcatc tatcagaaca ccgtcatcaa gcccgccatg
     1441 gtcggcatcg caggcccgtg ggtatccggc ggcgtggagt tcaactggcc tcagcaccac
     1501 cgtcccacca ccttcatgcc cctggaagcc accccgaaga agaacgccga cggttccgtc
     1561 accgtctgga tgggcgaagc cgagcccttc caccggatgc gcggcatggt gggcatcacc
     1621 gtgtatcccg gccgctccta tgtggaagcc aaggccgtgg tttacaaccg caccgattct
     1681 cctctgccct tcatgtggtg gaacaatctg gccgtgcggg ttcatcccca gtacaaggcc
     1741 actttcccgc cggatgtggc ttacggcaac gaccatgacc gccgcgccgt cattcccttc
     1801 ccggtgatga agggcgtgta caagaccgcc cgtcccttcg attacggcaa gggcacggac
     1861 gccacctggt tctccaacat caagctgccc acctccgtca tggtgatgcg tggccagagc
     1921 cagatgaatt tnnnnnnnnn ntgccgcaga cgcgggcacc gtgaccgttt cctgccacca
     1981 caccagcgtg ggcaagaaaa tgtggacctg gggcgacggc cccttcggcc gtgcctggtg
     2041 cgccaacctg accgacaacg gcgaccgcta catcgagctg atgaccggcg tttacaccga
     2101 taaccagccg gacttcacct atatcatgcc cggcgaaacc aaggtgttca cccaggtatg
     2161 gtaccccatc catgccatcg gcgaagccaa gaacgccacg ctggaaggcg cgctgtccct
     2221 gaacgtgaag gacggtcaga tccatttcgg cgcgctgccc acctgcgagc gcaagggcgc
     2281 cgtcgtccgt ctgaccctga acggcaaggt gctttacgaa aagaccgccg atctgtcccc
     2341 cgaaaacgcg ctgaccgact cctgccccct gcccgagggt gcgaaggaga ccgacctgtg
     2401 cctgaccctg agcgacgctt ccggcaaggc gctggtcagc tatcagccca tcgacatttc
     2461 caagaagcag ccccccaagg cccggcccat cccgccggat cctcaggatg tggccaccgt
     2521 cgaggagctc tacctccacg gcgcgcatct ggagcagtac aagcatcaca cctatgtgcc
     2581 cgaggattac taccttgagg gcctgcgccg ggacgccacc gacctgcgct gcaatctggc
     2641 catgggccgc tccatgatcg agaagggcga ctacgtcagc gcccgccgct atctggacgc
     2701 tgccattgcc aagctgaaga tgcgcaacga caaccccgcc gacaccgaag ccatctacca
     2761 catggcccgt ctggagcggc tggaaggcca tctggacaag gcttacgatc tgttctgggc
     2821 cgcggcgtgg cagtacagct ggcgcagccc cagctattat gaaatcgcct gcatcgatct
     2881 gtgccggggc gatcaggcgc aggccatcga gcatctggag ctggcgctgg aaaccaacgc
     2941 ccgtcacttt gccgcccgtg ccattctggc ctacctgcgc agcaacgcag atgacctgaa
     3001 ggccattctt gaaatcgcgc ctcaggacgg cctgacccgg ttcctgctgg gccgtctgac
     3061 cggcgaagcc acgctgagcg cctatacgct gggccgcagc gaggacatga ccgacatcgc
     3121 gctggatctg gctcacgccg ggctgaaggc cgaggccgaa gcggcgctcc gcgcctgcac
     3181 cgcccccagc cagatgctgt attaccatct ggcccacctg accggcgaag cgccccagca
     3241 ctgcagtctg gattactgct tccccaaccg gctggaggac atccccgccc tgcaggcgga
     3301 cgaatggcag gcgcaatatc tgctgggctg cctgtactat gaccgcatga actacaccgc
     3361 cgccatgacc gcgtgggaaa aggcggagaa gctgaaccca gcggatgctt acaccaagcg
     3421 ctgcatggcg caggcgtgct tcgaccatct gagccagccc gcccgggcgc tggagctgat
     3481 gaagcaggcg ctggaactgg cccccgacaa cgcccgcatt ctctacgagc ttttgcagat
     3541 ggagaagaat ctgggcttct cggtgaagga tcgcctccac ctgctggaga cccacagtga
     3601 gctggccaag atgcgggatg actgcttcct ggacgagatc atcctctaca cgcaggacga
     3661 gcagtacgaa aaggcccgag agctgctgct gagcaagcgg ttcaacatct acgagggcgg
     3721 cgaaggcaag ctgacccgcc accacggctg gctgtacacc ctgatgggcc agaaggcact
     3781 ggaagcgggc gatactgaaa aggcactgga atggctgaag accggcctga tctatcccgc
     3841 caattacggc gaaggccgcc actacagcgc gcaggagggc aacgtctact actacaccgg
     3901 cctgtgccat gaagcgatgg gcgacgccgc caaggcgaaa gaggcctatc aggaagccgc
     3961 cggtcagccc agccagatca ccgaaatgac cttcttcacc gcgctggcgg aagccaaact
     4021 gggccgggaa gaagacgcgc gcaagacctt ccggagcatg gtggaggaag gcgaaaagcg
     4081 gcaggcctcc agccaccgct ggggctactt tggcgtaggc atggccgcgc ccctgcccag
     4141 cgagctggac atcaagcgca tgaacctgat cgacgcgcac ctgctgatgc tgctgggcaa
     4201 agcgggcctc gggcaggatt accagagcga tctggacgct ttgaaggtct acgatcccta
     4261 caacagcaag ctggacttct tccagaagct gggactggtg aaataaacca gacatacaaa
     4321 aacacgggct gcgggaaaaa tccctgcggc ccgtgtttga ttgatgcact tacagcgcgg
     4381 caaatacctt cgtcccccga tttttcagcc atgcatacag cagcgcgcac agtgccgccg
     4441 tcagcagaca gaaaacggct gggatcaccg cgggatgcag attccggccc agcgccatgg
     4501 ccgctccgcc cggaagaagg gcgtaggccc agccgcctag cagggccaag gccacgctgc
     4561 caccctgctt gacgggggta atttcgctgg tccagtgcag gttgggcatt ttcaggccca
     4621 tggtcagccc gaacagagcc aatagcagca cgctcagcag actgccggcc gccgccagca
     4681 gcgacagcgc cgatgtcgtt catggaggcc agcagacagg cgaccgagat cgccagcgcc
     4741 gccagcacgc tgtccgcgcc gggaagaacg gccttcagaa gccccaacca ttctccgcct
     4801 ttgagcagga gcagcacgcc tgccgccggg atcatcagca cgcccagacc gcagttcagc
     4861 atgtagttgg ggctggaaac aaaacgccgg cattcttttc ccagcagcgc tgtaggcagg
     4921 ctgcataccc gggctttctt ctcccggtag cgggccttgc ccgtgctgcc gccggaggtc
     4981 gccagccgca ggaagctgcg ggaaagcagc agccaggtca gggccgtcag caggacgacc
     5041 g
//
LOCUS       DAWAEA010000052         5025 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2631_length_5025_cov_1.994769, whole genome shotgun sequence.
ACCESSION   DAWAEA010000052 DAWAEA010000000
VERSION     DAWAEA010000052.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2631_length_5025_cov_1.994769"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    720..729
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1165 a         1392 c         1375 g         1083 t
ORIGIN      
        1 ggcgaaggtt tcatcccggt aataaaccac tgcttcgtca ggattttccg tagcgtacag
       61 gcgtttgccc ttgccctccg caatcagctc cgtaatctga tccatgcttc aaccctctta
      121 catcgttatg gcttcgtcaa aaagccgcct tttccgctct attgtagcat cttccgccct
      181 caagcgcaaa tcacacggta atatttttca cccatttgcc atttttgagg atccacaagg
      241 ccgggaacac cttggcgtac atcaggaact gtacgatcag caccgctacc caaagggaaa
      301 tctttcccgg caggaaaatg cccatcagca cggcggcggg cacgggcacc gcccacatat
      361 agccgctgtc cagcagcatg gcgttgcggg tatcgccgcc ggcacgcagg cagaagaagc
      421 agaatccgta cacgaactgc agcgggcaca gcacgctcat gatgagggag atcagcgtcg
      481 ccgtgtgcct gagttccggc gtcagctgat aggcctgcgg cagcagcacc gacagcaggc
      541 aggacagaag cattcccgcc atggcgatga ccacgtccag ccccagcagc ttcttgcagc
      601 tttcccgggc ctccttcagc cgattcgcgc ccagctcggt gccaatcagc acggagaccg
      661 ccgccgaggt gccggaggcc aggatgaagg cgaacatatt gcacacctcc gcaatggagn
      721 nnnnnnnnnc cgggcgtagc accagaagta caggttcagg cccacgctcc acaaaagctc
      781 gttagccgtc agcggcgcgg ccttcacaaa gaaggtgcgc acggttctgc cgggcaggcg
      841 cagggcttct ttcaggttcc agttgaaata catccgtttg ccgcaaagca gcaccagata
      901 gaccagcatt tccagtgtcc gagcgatcaa tgtgccccac gccgcgcctg ccacgcccag
      961 cgcgggcgcg cccagcttgc cgaagatcag aatatagttg cagacggcgt tgggggtaat
     1021 ggtgcccaga ctgaccagca tgggcagccg gttcttgccg atggaacgca gtgaaaacac
     1081 acatacgttg ctgatggccg taggcagata gctgacgctg acccccgcca gataggcaag
     1141 gcccagcagg acggtgtttt catccttcac aaacagtccc atgatctgcc ggggcagcag
     1201 ctgcagcagc acaaaaaaca ccgcggagat cgccaagctc aaaaacacct gcagggaaaa
     1261 caaaccctga caggtcttgt ggtcctccgc gccgtagtac tggctgatca tcagccccgc
     1321 tccgccggtc aggccgaaaa gcagacagtt gaagatggtg tagggtttat tggccacgct
     1381 gacggcgctc atggccagcc cgttcagatc cagactgccc accatcaggt tatccaccat
     1441 attgaaaagg ctgttgatga gctgctgcac cgccacgggc agcatcacgc ccagcgcacg
     1501 gcggcaaaat tccttatccg cccggtagag ccttaatcgt tccgaaagct gcatctttgg
     1561 tcaagctccc atttcttcca taatattaaa aacatagcgc cgcacgcgcg ccaaaattca
     1621 ttgtattctt tctctttgcg ctttgtcaag ccggaaacgg ccgtgcgttg ccaatccttc
     1681 caaaacatcg tataataaac cttcgtgaaa acccgttcaa aggagggatg acgttgtacc
     1741 gcattttcat cgtggaggac gacccggtca tcgtgtccgc cctgtgcgcc catatggaaa
     1801 aatggggctt cgaatgccgg ggcgtggagg atttccgcaa agtaacggag gaatttgccg
     1861 cctatgcgcc ccatctggtg ctgatggata tttcccttcc tttttatgat ggctaccact
     1921 ggtgcgccga gatccgcaag ctgagcaaga ctccgatgat tttcctttcc tcagcggcgg
     1981 aaaatatgaa cattctcatg gcgctcagcg cgggcgcgga cgatttcgtc cagaagcctt
     2041 tcgacctctc cgtgctgctg gccaaggtgc aggctcttct gcggcgcacc tacgatttcg
     2101 ggcaggcggc tcccacgctg ctcagccatg agggactttt gtacaacccc gccgacggca
     2161 cggtggaatt tcaggggaaa accgtccccc tgagcaagaa cgaaagccgc attctgggcg
     2221 ttctgctccg gcacaaggga gagaccgtca gccgggagaa cctgatgaac gccctttggg
     2281 agacggactg ctttgtggac gaaaacaccc tgaccgtgaa cgtgacccgt ctgcgccgcc
     2341 ggctggaaag catcggcgcg ccggacatga tccagaccag aaagggcgtc gggtatctga
     2401 tctcatgaaa agaaagctgt ttggaacggc ccttggaggc tatcttcggg aaaaatccgg
     2461 ctggcttctg ggctttgcgg gcatgatcgt ctgcttttcg ctgatcggct gggcttgcgg
     2521 tctgccgggt tccgtggcgc tgtacggtgc gctgctctgc ggcgcagggc tgctggcagg
     2581 atgcattatc ggcttctggc gacacctgcg gcggctttgt ctgcttcggg agctggacgc
     2641 gctgcgggac cccgccgagc tgccgcggcc ccgcacccag atcgaaacgg agtacgaccg
     2701 gctgctgcgg cagctgtacc gggaaaaggc cgagctgttg gcggaaagcc gtcaaagcag
     2761 ccgccgccag ctggacacct tctccctgtg gacgcaccag attaaggtgc ccatttccgc
     2821 tatgaagctg ttgctgcagg atcggcccga cgcggccagc gccacgcttt tgtgcgagct
     2881 gttcaaggtg gagcagtacg ccgacatggc gctgaattac gcgcgaatga acgcctcctc
     2941 caccgactac gtgctgcgcg cctgcgatct ggacgcgctg ttgcggaagg tactgcgccg
     3001 gttcgcgccg ctgttcattc agaagaagct gcggctctgc tacgagcctg tgtcctattc
     3061 gctgctgacc gacgaaaagt ggttttcctt cgccgtggag cagctgcttt ccaacgccgt
     3121 caaatacacc aaagccggtt ccgtcaccgt gacggtggac gcggaaaagc agcttttgac
     3181 catttccgac accggcatcg gcattgcccc ggaggacctg ccccgggtat tcgagcaggg
     3241 ctacaccggc gaaaacggtc gggaacagca gcgggccacc gggctggggt tgtacctgtg
     3301 ccgggaggcg ctgacccggc tgggccattc cattcggctg gatagtacgc ccgggcaggg
     3361 aaccacggcg tttgtggggc tggagcggga cgaacgggag tatgaataaa aaaacagaga
     3421 agactttttc agaaacagag aaaggaaaat gatatgttct catacaccgt ggaaaagaat
     3481 gcaagcaaac agacctttga aatggtatgc cgtctgatag aatctcactt cacagggatc
     3541 gttaaggaaa agctgctcga ggacgtggac ggaacagcca ttcaattcta ccattttgat
     3601 agcaaaacca tcaaagtatg caatgatttc gaaatagatg ctgtatatgt ggattctgaa
     3661 atagaattgg gatgtgttct atatgaataa agcattttct aaataggagg taaaaaaatg
     3721 atcaaaaatt tacaagtgtc tgatctcgtt gaattttttc aggatattcc gatttacgtg
     3781 ggagatgaac aaggctgcga aatgcttttc aaaaaagagt atcctcatgg tttgtctttg
     3841 acggtctact tcgatgtcta tggagaaaaa agtgaaatga ccatttcctg tccggaattt
     3901 cccatcgcaa catttttgtc gagaattgaa agattagaaa tcaaatcgaa cgttatccgt
     3961 tttatatgcc gcgatcagtc tgtagctgag ctgcaagtag aaccaagtcc tttcttaaaa
     4021 gtccatggat gttctgttcc gaaagattcg atttaattta tgccgcttgt aaaaagccca
     4081 aatgctgata tgacagatac agccaatctc tttgcaagcc gttgagtgac ctgaaacgga
     4141 ggtattatgg tcagtctgga aagagcaaaa gaactcgcca taaaagaatt ttccgaatac
     4201 ccgatcaggg agataatcga cctcggaaac cgctgggctt tttgctatga ttccggagaa
     4261 ccgccggttc ccggcgtctg cgcagttacg gtcaaaaaag gagacggaaa aataggctat
     4321 ctgcctgttc caccgttaga gaatcttgac attctgagca acggaattgt tattcaatcc
     4381 atcaaatagc acaactcatc tttctccgct tatttctttt aagttttgcc cgctgcgcac
     4441 aaaccttacc cttttgtaag gttgcaagcc cgaattgtaa gccaacgcca tggatgcgcc
     4501 gcttcgcctt ctgctatact ttcggagcgc ttggcgcaaa ggaggttttt tgcaatgtcc
     4561 atactcaagg tcgaacattt gaaaaaggtc tacaccaccc gcttcggcag ccagcaggtg
     4621 caggcgctcc gagacgtgaa tttttcagtc gaggagggcg agttcatcgc catcatgggc
     4681 gaaagcggca gcggcaaaac cacgctgctc aacattctgg cggcgctgga caagcccacc
     4741 gagggccatg tttttctggg caaccgggag ctttccgccg tcagtgagaa ggaaatttcc
     4801 gctttccgcc gggatcatct gggctttgtc tttcaggatt tcaacctgct ggacaccttc
     4861 agcctgcggg acaacatcct gctgccgctg gtgctggcgg gcacgcccta ccgggaaatg
     4921 gagcagcgtc tgaagccgct ggcggacaag ctgggcatca cttcgcttct gtccaaattt
     4981 ccctatgagg tctccggcgg acagaagcag cgggcggctg tggcc
//
LOCUS       DAWAEA010000053         5001 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2648_length_5001_cov_2.051557, whole genome shotgun sequence.
ACCESSION   DAWAEA010000053 DAWAEA010000000
VERSION     DAWAEA010000053.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5001)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5001
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2648_length_5001_cov_2.051557"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          751 a         1583 c         1543 g         1124 t
ORIGIN      
        1 ctgtacctgc ttcaaaacag acgcaatgtg gatgccgtat tcgatgttgt tgtccagctc
       61 gaagaggatt tccggcgcgt agcggataat catctgctcg cccagctggt gacgcacaaa
      121 cccggcagcc tttttcagcg cgtccatcat gggcttgcgg ttctcttctt ccagaatgct
      181 gacgtaaatt ttggcatagc gcaggtcacg ggtcacgtcc gctcgggtga cgctgtaggt
      241 gccctgaatg cgcggatcgg acaggtcttc ccggataatg cgatccacct cccggcgaac
      301 ctgctcgctg atgcggtcaa tacgctgata actcaatgcc tttcctcctt tggcagcaaa
      361 ggttctgtga gggaaggccc gtccttcccc ctattccaaa attggccgcg cagcgcgcgg
      421 ccaaaataag caaacctgtc cggggtccag aggtactacc cctggtcagc gttcgatttc
      481 ttccatgatg aagcactcga tgatgtcgcc ctctttgatg tcgttgtagc cgtcgaagct
      541 gatgccgcac tcgtagcccg cagccacttc cttcgcgtcg tccttgaagc gcttcaggct
      601 gttcagcttg ccttcgtaaa ccacaatgtt gtcgcggagc agacgtacct gcgcgttgcg
      661 ctgcatcttg ccgtcctgca cgtagcagcc tgccacggtg cccgcgccgg tgatgcggaa
      721 ggtgttgcgc acctcggcat ggcccagcag aacttccttg tattcgggag ccagcagacc
      781 cttcatggcc ttctcgatgt cttcgatggc ctgatagatc acgcggtaca gacgaatgtc
      841 cacaccctcg cgatccgccg cttccttggc gttgttgtcc gggcggatgt tgaagccgat
      901 gatgatcgcg tcaaacgcgg acgcaaggtt cacgtcgtcc ttggtaacgg cgccaacggc
      961 gctgtgcagc acgcggacgc gcacctgatc gttgctcagc ttctccagcg cctgcttgac
     1021 ggcctccacg ctgccctgca cgtcggcctt gacgattagg ttcagggtgg tcatcttgct
     1081 ctggtcgatg cggctgaaca gattgtccag cgacaccttg gtagaggaag ccacccgggc
     1141 ggccttcagc ttgtcgcggc gttcctcgac cacctgacgg cccagacggt cgtcttccac
     1201 ggccatcatt tcgtcgcctg cgctgggcac atcggtgaag ccggagatct ccacaggcat
     1261 ggagggaccg gcggacttca cgcgctcgcc cttgtcgttg accatggcgc ggacgcgacc
     1321 gctggccaga ccggctacca cgttgtcgcc cacatgcagc gtaccgttct gcagaaggac
     1381 ggtggccagc gggccgcggg ccttatccag cttggcctcg ataataacgc ccttggccat
     1441 gcggttgggg ttggcgcgca gctcgcgcac atccgcctcc agcaggatca tttccagcag
     1501 gttatccacg ccttcgccgg taagggcgct gacgggcacc atgatggtat cgccgcccca
     1561 gtcctcgcag accagaccgt agttggtcag atcctgcttg atgcggtcgg ggcttgcgcc
     1621 tggcttatcc atcttgttga tggcgacgat gatcggcact tcagccgcct tggcgtggtt
     1681 aatggcctcg atggtctggg gcatcacgcc gtcgtaacac ggccgtcgat ggtgacggta
     1741 tacgcgccga tatgctgcgt aatgccgccc gcttcgccgg cagtcacgtg agaatgacgg
     1801 atgtagtcca gcagcgaggt cttgccgtgg tcgacgtggc ccatgatggt gaccacgggg
     1861 ggacgggtga ccagatcttc ctcagcgtcc tcgaagtcgg tctccttcag gtcatcctcg
     1921 gcggtcttgg ccagattcag ctccagcgtc acgccgaagt cgctgcagac cagactggcg
     1981 gtatcgtagt ccagttcgct gttgatgttg gccatcacgc ccagcatcag cagcttcttg
     2041 aggatctcac cggcgggctt gccgatacgc tcggtcaggt ccttgacggt gatggtctcg
     2101 gcggtcatgt aggccttttc gatcttgatg ggggccatca gctgctgggc gctgggtttc
     2161 ttgacccggg cccggcggcc gccgcgcacc acgtcgtcgt cgtagccgtt gaactgatcc
     2221 cgggccagct gcttgcggtt tttggccacg cgctccgggt catgctgacg gacatagttc
     2281 ttcttgttgg ggtcataatt gctgacccgc tccttctcca tcacgggagc cagatcaacg
     2341 gagggcttgg agcgtcctcc gcccatggga ggacggttgc cgccagccgg acggccgaag
     2401 ccgcctgcgg ggcgctgcgc tccgccctgc ggattggcag gacggccata gggaccctgc
     2461 gaattggcag gacggccata cgggccttgc gggttcgccg ggcggccata cggaccctgc
     2521 ggattggcag gacggccata cgggccttgc gggttcgccg ggcggccata cggaccctgc
     2581 ggattggcag gacggccata cgagccttgc ggattcgccg ggcggccata gggaccctgc
     2641 ggattggcag gacggccata cggaccctgc ggattcgcgg gacggccata ggggccggcg
     2701 ggacgggggc cgttgcccac aggacgcgcg ccctgctggg gaacgggacg agcctgctgc
     2761 tgcacgggcc gcggagcctg ctgggatgcg ggagcaggcc tctgcgccgc ctggtgggca
     2821 gcagcgggct gcgccggagc attatgctgc tggggcgctg ttttgacgct ggcaggctgt
     2881 gccggggcag gcacggcctg agcgggcttt tcgggtttgg ccgcgttctc cttcgccgtc
     2941 tggggggctg cggcgggagc gggcttcttt tcttcctgaa ccttttgttt ctcttccagc
     3001 ttttgcacgg gctgtttctc ctcctgcttc acgggtgcgg gagcaggctc cttcttttct
     3061 tcctttgcct taggggctgc tgccgggacg gttttttctt ccgccttcgg cgcaggggac
     3121 gcttcttttt tggtttcgga cgcttccgct ttgacgggag cggcgggctt ggcgggagct
     3181 tccgccttaa cgggcgcttt ctccgtggcg ggcttttctt ccgccttcgg cgcaggcgcg
     3241 gtctggacgg gcttgggctc gtcatccgtg tcaggcatgg tgaaggcctt ggcgtgctga
     3301 ctgatcagcc gctcctgctc cgcctgcttt tcttcggcgc ggcggcgctc ctcctcccgg
     3361 gcaaactggt tttcctgctt tttcgccagc tgcgccagag catccgcgtt cttgcgcatc
     3421 ccggagagct tgctcagcgt ttcttccgcg cccttcaaca gttccctcgt tgcatccttg
     3481 agctttactt tggtcatgta ccccttgcac ccccatgtat tttacagtcc gcggggactg
     3541 tcaatgttcc tgaaaataag tcaggagttt atcggccatt cgcccggcag ggacggagat
     3601 caccatccgt ccgggcttgc cgatcgcgtg ccccagcgcg ccggggctga tacggtaaag
     3661 cggcacctga tgactggcac aggcgtcctc cacccgcttg agcgtgtttt cggacgcgcc
     3721 cgcgtccagc agcgccaaag cgcattcgcc gccgcgaatg gcggcaacgc aggcttcctg
     3781 accgctgacc acctgaccgg cccgcataca caggcccaga aaggacagtg ccctttcctc
     3841 cgcagtttcg ttcatgcctt gctttcctcc agcagacgat tcatggtctc ggtcaggctc
     3901 tgattgacct ccggggtcaa cttcacctcc agctggcgat ccagctggcc ctgtttcagc
     3961 gcccgcttta gacagtcggc gctgtagcag cagtacgcgc cccggccggg ctttttcccg
     4021 gtgggatcca gactgacctc tccctccggc gaacgcacta cgcgcaaaag ctctttcttg
     4081 ggcttcatct gccggcagcc cacgcacatg cgcatcggaa ttttcttcgg ctttacgggc
     4141 atccgaaggc cctccttctt tggaatgaaa taggcggctt acagcgtgtc gtcgtcctca
     4201 tcgtcgccgc tcagttccag cggcaggtcg cgcagctccg gcagaccgga ttcggccatg
     4261 gcttccggag tggcaggctc attgtacaac gcctgatagc ccatggcaag attttcttcc
     4321 atcggctctg gctcttgata tcgatgcgcc agccggtgag cttggcggcc aggcgggcgt
     4381 tctgtccttc cttgccgatg gcgaggctca gctgattatc cggcaccacc acgcggcagg
     4441 ccttctccgt atcggagatg aacacgctga gcacgtgggc ggggttcagg gcgttggcga
     4501 tgaactcggc ggggtcggcc gaccacttga tgatgtcgat cttctcattc ttcagttctt
     4561 ccaccacttt ttccacgcgg ttgccgcggg ggccgacgca ggcgcccacg gggtcgatgg
     4621 tgacctcggt ggaatggacg gccatcttgg tgcggctgcc ggcttcccgg gcgatggact
     4681 tgatctgtac cacgcccgca gcgatctccg gcacttccat ctcgaacagg cgcttcacca
     4741 gtccgggatg aatgcggctg acgcgaacct gcggcccgtg gagcatttca cggccggtgc
     4801 ggttcacttc cagcacatat actttaatgt ggtcgtcgat gtgatattcc tcgccgggca
     4861 tatattcgct gggctccatg atgccctcgg tgcggcccag ctccacatac acgcccttgg
     4921 gctccacccg ctgcacgatg gcggtgagga tctcgttctc cttgtcggag tattcctcat
     4981 agatgcggcc ctgctccgct t
//
LOCUS       DAWAEA010000054         4997 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2650_length_4997_cov_2.253339, whole genome shotgun sequence.
ACCESSION   DAWAEA010000054 DAWAEA010000000
VERSION     DAWAEA010000054.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4997)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2650_length_4997_cov_2.253339"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2236..2245
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1046 a         1516 c         1476 g          949 t
ORIGIN      
        1 caaatcccaa tccgcgccgg agtcggcgcg tcaggcccca ccggggcagg ggtttgcgcc
       61 tgcgggcgca ctggtggctt tccgctcgcc cccagtcccc ttcggatcgc cacctgaagc
      121 aatcgagcgc tccatagggc tgcaagccct ctccccgcgt cacagtcggc gcgtcaagcc
      181 ctcggcggga actttttaca gcaaaactgg ctttttgcca ccgcttgccc aaatgaaacg
      241 aacgctgccg gtaataaaac cggcaagatt ttttaccccc caatcccttc ccttatgaaa
      301 ggagaccccc tatgcctacc ccttgcgatt ccgttatccg cgtcaccctc gatgggaaaa
      361 ccgacgcgtt ccctgtgaat accaccgtcc agaccgtgat cagccgcctg ctggacgcgc
      421 ctgcggctgc cgcggtcctc ggcgtaatgc agggcggcgt gtgcagggag ctgaacgaat
      481 ccctgactaa agactgcgaa ctcaccagcc tgacctaccg ggacgaagaa gggcggcgta
      541 tttatgaacg ctctctgcgc ttcctgatgc tggcggcgac attgcgcctg catcccggca
      601 gaagggtgcg ggtgctgaac tccgtgggct acgggctgta cgtccggctg gagagcgagc
      661 tgacccacgc ccaggttcgg gagatcgagg cggaaatgcg ccgcatggcc gaagctgatc
      721 ttccctttga aaaggaacat tggacccggg aacaggccgt gcagtatttt gaaaagatgg
      781 gctggacgga taaggcggag ctgatgcgct accgcccccg ccgggagatc atgatgtacc
      841 gcatgggcgg tctgtgtgaa tatttctacg gcgcgatgct gccttctacc ggctgggtga
      901 aaaccttcag cctgaagccc catttccccg gcatggtgct gatgatgccc tcgcccgccg
      961 acccgagcca gtcggccaaa tatcaccctc ataccaagtt tttgcgggtc ttcggccagt
     1021 cgcaggaatg gtgccatatt ttgggggcca ataacgccgc cgacgtgaac cggatgattc
     1081 aggagggcaa gctgcgggag ttcatccggg tcaacgaagc cctgcacggc aagtccctgt
     1141 cctatatcgc cgacgagatc accagtcgcg gagccaaggt ggtcatgatc ttcggcccgt
     1201 cctccagcgg caagaccacc ttcgccaacc ggctggccgt ccacctgcgg gtgctaggcc
     1261 gtcagccccg gctgatctcg ctggacgact tctatctgga ccgctccatc ctgccgctgg
     1321 agaaagacgg aaagcctgat ctggaatcca tcgacgcgct ggacgtgccc cttttgaccg
     1381 aaagcctcga agcactgctt agcggcgaaa aggtggaaat gccccagttc gatttccaga
     1441 tttctgcccg cagccccgag actcatcccc tgcagatggg gccggacgat attctgctgg
     1501 tggagggtat ccacggcatg aacgaccgca tcacctcgga aattcccgag cagctgatgt
     1561 acggcattta cgtcagcgcc ctgagctgca tcaatctgga cgatcataac cgcatccgca
     1621 ccacggacgt gcgccttctg cgacgcatcg tgcgggatgt gaagatgcgc aatacctccc
     1681 ccgaaaagac catcgacatg tggcccaagg tgcgggccgg ggaggaacgg tggatcttcc
     1741 cctatcagga gcgggcggac gtgatgttca acacctccct gcattacgag ctgccggtgc
     1801 tcaagaccat cgcctatgaa atgctgctgg ccattcccca aagtgcgccg gaatatctgg
     1861 cggcccaccg gctgctgaaa aacctcaatt atttcctgcc cgtggatgga aaggtcatgg
     1921 acgagatccc cccgctgagc atcctccggg agtttgtggg cgaatgttcg ttttacgaca
     1981 ttcattgaca gaaagcagac aatatagtat aaataagcgg ttatcttcac cctcccaggc
     2041 aacgaaagga tgttatccca gtggaagtac tgtcccagat cctggaaata ctcaacgcta
     2101 ttctgagcgg ctggttgatg ctgatgctgg tgtatcagct ggtgattacg tttttcggct
     2161 acaagcgcaa tacgaaggat tatcaggacc acgcgccgct gatgcggttt ctggtgctgg
     2221 tgcccgccca taacgnnnnn nnnnngaggc cgtcatcggc gacatcgttc agaacctgaa
     2281 cgagatggag tatccccggg aaatgtacga tttctacatt ctggcggata actgcaccga
     2341 ccgcacggcg gagatcgccc gggggctggg ggccaacgtg ctggaaagct ggaaggacag
     2401 cccggacgcg cctaccggca agcccatcgt gctgcaaaag gccctccaga cgctggggga
     2461 ctatcagaac cggtacgatc tggtcatgtt tttcgacgcg gataatctga tcgacaaaaa
     2521 catgtttgcc gaggtcaaca gccagtatct ggacgccaac ggtcaggcgg atattatcca
     2581 gtgctatttg ggctgcaaaa acaaaaaagg catcgtggcc atgttctatt acatgaccta
     2641 tacgatcacc aaccgttttt tccagtacgc caagagccgt ctggggctga acagcgtgat
     2701 cggcggcacg ggctttgccg tgtccgcgga gtacctgcac cgccggggcg gctggacggc
     2761 catgagcctg accgaggact tcgaactgca gatcgagtcc acctgtgagg gcaagcgcat
     2821 cctgtggaac cacaacgtgc gcatctatga tgaaaagccc accgccttcc gggccagctt
     2881 ccgtcagcgc acccgctggg cccagggcca ctggttcgtg gcgttcaaga acacgggcaa
     2941 gctgttccgg gcgctgttcc agaagaagat ccgcttctcc gagttcttct ccaccttcct
     3001 gtacatgtac agcctgacgc cctacgttat tctgatgttt cagctggcga tgggcgcgct
     3061 gatgcagatt tttgtgtggc tgggctggct tcccgctacg ccgaagaccg cctcgcccaa
     3121 cagctggctg accatgaact ggcccagcat tgtgctgttc ttctattcct ttgtttttct
     3181 gttctatctg gcggactgga tggacaaccg ctgccgtatc gagtggaaga aattcgtgcc
     3241 catgctggtg agcgtgttcc tgaacacggt tctggcgggc tgggcgcagg tggtcggcct
     3301 gttcaagcac cgcaaacaga accagtgggt caagacggag cataagattc acgataagga
     3361 cgatatgacc gtgaacttcg acgtggccgc gcctgagccg gaagcggaag ccgctgccga
     3421 agaagaaacg gtttcctgcg aagaaaacgc cgcagcacgt ttacattccc ttcttccgct
     3481 ggtaaaatag ctgcaattaa aggaagaaag ggtggtttta tgctggaaga gaatctccgc
     3541 accgggctca cgttcgacga cgtgctgctc attcctcgtg aaagccacgt gctgccgcgc
     3601 gacgtggaca cctccacgat gctgactccc accatcaagc tgaacgtgcc gcttctgtcc
     3661 gccgccatgg atacggtgac ggacgcccgt ctggccatcg ccatggcccg ggaaggcggt
     3721 ctgggcatca ttcataaaaa catgacgatc gaggagcagg ccgcgcaggt tgacaaggtc
     3781 aagcgcagcg agcacggcgt gatcaccgat cctttttatc tgtctcccga acacctgatc
     3841 cgggacgcgg aggcgctgat gtcccgctac cgcatcagcg gcgtgcccat cacccgtgga
     3901 aagaagctgg tgggcatcct gaccaaccgc gacctgcggt tcgagacgga cgattcccgc
     3961 cccatcggcg aggtcatgac cagcaagaac ctgatcaccg ctcccgtggg caccacgctg
     4021 gagaaggcca aggagctgct ggcggcccac cgcattgaaa agctgcccat cgtgaacgaa
     4081 gagggcgagc tgtgcggcct gatcaccatc aaggacattg aaaagagcac caagtacccc
     4141 aactccgcca aggatcacaa cggccgtctg ctgtgcggcg cggccgtggg cgtgaccaag
     4201 gacgtgatgc agcgggtcga agcgctggtg gccgctaagg tggacattat ttccatcgat
     4261 acggctcacg gccataacag cggcgtggct gaggtgctgc gcatgatccg cgcggcttac
     4321 cccgacctgc ccatcatcgc gggcaatgtg gctaccgccg ccgccacccg tgccctgatc
     4381 gaagcgggcg cgaatgtggt caaggtcggc atcggcccgg gcagcatctg caccacccgg
     4441 atcgtggccg gcatcggtgt gccgcagctg acggctgtgg cggactgcgc caaggctgcc
     4501 gagggcaccg gcgtgaccat catcgccgac ggcggcatca agtattccgg cgatattccc
     4561 aaggccatcg ccgcaggcgc gcacgccgtc atgatcggca gcctgtttgc cggtgtggac
     4621 gagagccccg gagatatgga gatctatcag ggccgcagct tcaaggtgta ccgcggcatg
     4681 ggcagcctgg gcgccatggc gcaggcccac ggctccagcg accgctactt ccaggaggcg
     4741 gacaagaagc tggtgcccga aggcgtggaa ggccgcgtgc cctacaaggg accgctggcg
     4801 gacaccgtgt atcagatgat gggcggcctg cgctccagca tgggctactg cggcaccccc
     4861 accattgagg atctccgccg ggacggcgag ttcatccgca tcaccggcgc gggcctgaag
     4921 gaaagccatc ctcacgatat ccagatcacc aaggaaagcc ccaattattc ccggatgtaa
     4981 tgcaaatgga tcagcgg
//
LOCUS       DAWAEA010000055         4990 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2653_length_4990_cov_3.087943, whole genome shotgun sequence.
ACCESSION   DAWAEA010000055 DAWAEA010000000
VERSION     DAWAEA010000055.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4990)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2653_length_4990_cov_3.087943"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1088..1097
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2940..2949
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3655..3766
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4234..4243
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1099 a         1350 c         1273 g         1126 t
ORIGIN      
        1 gttcgcactc atcaagcgcg tcctgcgatg tatccgtctg cacaacgttg gaatggtgct
       61 ccagaatcag ccgatcgtcc cctacaaact tccgaatctc tttcgcgttc tgttccagaa
      121 tcgacagcag cggcgaggtg aaaatcaggc gttttttccc aaattgtttc gcgtgcgcca
      181 gtgcataccg cagtgaactg agcgtttttc cgccgccggt cggaacgttc agccgataaa
      241 tgccggacgc tttctccgca aacgctccgc acgtttcgga aattttctgc cgcgcgttgt
      301 cgatctctcc atcgtgcggc ttcccctgca gcttttcttc cagataggca agccgcgccg
      361 cccagaccgg cgtcatatcc tccggcaaat tcggcggtgt gcgttcctcc ataaacgccg
      421 ccgtatcata ccggtctccc tcgatcacag cggacagaag cagtcgggcc aacagtccga
      481 ccacgaaaaa atattcgtga tcatcgacgt attccctctc caactgttcc aaaatcggat
      541 cgatctcccc cactgcggaa gaaaagagcc gttctatttc ctgcttggaa attccctgtg
      601 aaaataagcc ctgcacagac tcctcatagg agattccctg cttttcagcg cggtacttca
      661 gcccgattcg ccgcgtggga tccacgcagt caaacaggcc atggtgcgcg ccgatagcaa
      721 acgcgagcag ttcggacgcc atgacgccgg tacgaaccgg ccgcaggcat tctgccgccc
      781 gccgcgccgc accgcgcagg tgctcccgca acgtctgcca aacgatttgc ctgccctctc
      841 gcttatgcgc ccaatattcc atcatatatc accagccttc gtttatttgt gaaaaacagc
      901 cgtatcaaat gttttctgcg gaaataatat aacccttgtt ttcgagaaag ttcagcattt
      961 tcttttctaa gtatttattt tgtaaataaa tttatatggc ggaataaaaa aatggtaagc
     1021 atatacttga ccacttgatt tttatgcctt gtttgatata ctagagaagt cttgcattac
     1081 acaagtgnnn nnnnnnntgt tacagtgaga cttggctggt tctgcagcac aaaaaacggg
     1141 cttgattttt cccactacgg tcggcactat gcctactcaa tcagcgctgt ttgggatgtt
     1201 tcgccgcggc ttggatattc ctgtaacaca aaagaccggc tctataataa aagttggggt
     1261 aatagccaaa aaggaagaca aatgattcgc aagctgataa agtgtcattg acgagaaaac
     1321 acacaggagg cgaatcactt gtccggaaaa gagtatacaa cagaactgct gaaattggaa
     1381 gatgcccaga ttgaaagaat ggaggaagcc gaagaagaaa ttattctgca aatcagcctg
     1441 aagcgaaaaa tgcatacctg cccaaggtgc aaagccaaaa ccgatcaggt gcatgattat
     1501 cgcattagaa ccgtaaggga tctgagtatc cgcggaaagc ctttaacact gctgtaccgc
     1561 agacgcagat acttctgtcc gtcatgcgga aaaagttttt cggagacttg cgcattcctt
     1621 ggcaaatatc agcgctttac ctatcaggtg atcgacagga ttatgcagct gctgcatcgc
     1681 agatggagca tgaaggacat tgcgaaggac acgcatacct ctgtcagcgg tgtctcgcgc
     1741 tgtctggcgc tttacccgca gggtaaacca agcgcgctgc cccgggtgct ttcctttgat
     1801 gaattcaaag gcaacgcaaa cggtgaaaga tttcaatgga tcctgaccgc tcccgaagaa
     1861 cggcgtatct tggatattct gccggacaga aaggtttcca ccatccaatc ctacttgcgt
     1921 tccttcccca accgacagga cgtacagtat gtggtgatgg atatgaacca agggtaccgt
     1981 gatattgcca aagtcttctt tccgcaggca aaaatcgtga ttgaccgttt tcatgtagcg
     2041 cgctactgca cttgggcgat ggacgatgtg cgcagagatg ttcaaaagca tctgttgcct
     2101 gcttcccgaa agcatttcaa acgctccaga aggctgctga ttgccagacg tgcctcgctc
     2161 tctgaggacg acagggccgc tgtggacgtg atgctccatt tctccgagag gctgtatcag
     2221 acttacgctc tgaaggagct tttctttcag ttcatggacg caaaaagcag ctccgccgct
     2281 gaggagttgc tctccaaatg gtttgatgcc tgcgaccgtc ttcagcttca cgaattctct
     2341 gcttgcagac ggatgctcaa aaactggaag ccctatatcc tcaacgcttt tgactgctct
     2401 tattccaatg cttttactga aggctgcaac aatgccatca aagctctgaa gcgcgttgcc
     2461 tttggctttc gcaatttctc caacttccgt gcccgcattt tacaggctgc gaatccttct
     2521 caccccaact tttgacaaag agccggaaaa aaacagaggc tcgtgacctg ttcgagagcg
     2581 ccggggtcca attccccgga ggttctatgc aggacggcct catccatagt atattaaaaa
     2641 atgattcccc tgtcaacagg aaatttgtgg agcagactga aacgacgcag tttaagcgct
     2701 ggtttgggga cagcaaggtg gtggacaggg acggcgagcc gctggtggtg taccatgcca
     2761 cggatgcgga ttttaccgtg tttgaccgtg acaaactggg ggaaagaacg aaacaaagag
     2821 atatagaaga ttttaacatg gatatggaag acagcgttgg gcttttgaga agcgcagagc
     2881 tgggcttctg gtttagcgaa aaaaatctgg cagaagatga atacggagga cgggatgtan
     2941 nnnnnnnnng caggaaagcg ggaacaccca agtcatcgcg ctccacggtg gaggcggcgg
     3001 gggcgggagt cgtttccgcc gcgggctgct gcgctgcttg ctggggctga gcgccggtct
     3061 gcggctgctg cgcctgcgca ttctgggcgg gagcctgata ctgctggccg tattccatct
     3121 gcggcatcac gggctgctgc acattgccgt actgcattcc ctgcaccggc tgctgcattc
     3181 cgtaatcagg ctggccctag gtctgccggg gctgatagcc gctgggctga ccatagctgg
     3241 catagtagtt ggaaccctgc tgcatcatgg gcggcatggc cgcaggggtg ggaatggaag
     3301 aaccgcccac ataaccgccg ccgaagtcgc tgtgcatgga ttcgtcctcc atgggcatat
     3361 tgaagctcat gtcctcatag gaagaaccag aggagaaatc ctgacgaccg gaagaaccat
     3421 aggtgctgcc ggaatttccg ttggagaagg aacgcgtcca gtcccgttcg ccgccgttct
     3481 tggattttgc gtcctccttc ttggggaagg gcgttttctc aaagccggta gcaatgaccg
     3541 tgatgcggac ttcatcgtcc agcgaatcgt caatgccagc gccgaagatg atgttggctt
     3601 cgctgtccgc ggcctgcatc accaaactgg cggcctcgtt gatatcgata atgcnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtcc agattgatga
     3781 gcgcgggcac ggcgatcaga tcgctgatgc cctgaatgcc ctgccgcagc acatcgtccg
     3841 caatgcggaa ggcttccagc atggtcgtgc ccttggtgac cacctgcagc agacggtcgt
     3901 tggggatcac gaccagcgta tcgacccgct gtttcaggtc gatcacgccc atttcggcgt
     3961 ttttcatgcg ctgcttgccc tcaaacagga agggcttggt gaccacagca atggtcaggc
     4021 agttcaaatc ccgggcaatt tcggccacaa tgggagccgc acccgtaccc gtgccgccgc
     4081 ccataccggc ggtgacaaat accagatccg cgcccttgat cgcctgagcg atctcctcgc
     4141 ggctttcctc cgcggcacgg cggcccacat ccggaaccgc gcccgcgcca agacccttag
     4201 tcagtttttc accaatctga atggtggtgc tggnnnnnnn nnnctcatgg ccagcgcctg
     4261 acgatcggtg ttgatggcga taaattccac gcccttcaag cccgcgtcca ccatgcggtt
     4321 gaccgcgtta ttgcctgcgc cgccgcagcc gattaccttg atcgtagcaa agctctgttg
     4381 atcctcgatc tgaaattcaa gcacagtgca tccccctagt ttttgtttcg ctcaggtttt
     4441 atccgctgct cagccgccca agaggcttcg gaagaagtcc ttgatgccgc ccgaaaaacc
     4501 gcttgcggga acatttctgg attcaatggt atcgatgatc aggtccatca ggcccatggc
     4561 gctggcgtaa atggggctgg acagcttaat ggtacgcttg acagtgtcgc gcacaggctt
     4621 ttccagcttg gccgccagat aatcccggcc gccccggttc aggctcaggc cgccgccggt
     4681 tcaggctaag gccgccgccg gtcaggtaga tgttggacca atcgcccagc ttcatgccgc
     4741 tttcgtcgat gcattcccgg atcatctcgg cgatttcgtc cacccggggc tccagcacct
     4801 cggccacctg ctcctgcgta aaggtatccg ctttcacgcc gtcgccgccc gtcaccgtat
     4861 aggattgcga gatggtggaa attccataca catattcgcg cttgatctgt tcggcggatt
     4921 ccagcgaaat gttcaagccc tcggccacgt cggcagcaat atgtccgccg cccacgggaa
     4981 gcgtcttctg
//
LOCUS       DAWAEA010000056         4933 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2693_length_4933_cov_1.885404, whole genome shotgun sequence.
ACCESSION   DAWAEA010000056 DAWAEA010000000
VERSION     DAWAEA010000056.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4933)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2693_length_4933_cov_1.885404"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    264..451
                     /estimated_length=188
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    800..904
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2107..2236
                     /estimated_length=130
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          761 a         1464 c         1280 g         1005 t
ORIGIN      
        1 cggcggggca gcccaccaca aaggcgtcct caggccccag cagcccgtta tcctgcagat
       61 cccgcaccgt gccccgcaca aagcggacaa tgtcctcatt ccgggccggg tcgtaggtaa
      121 agcggctctt gaagcgcacg ctgagcccgt cggccagcgc gtctgtatag gcccgttcac
      181 ccacaacgat ttccccgttc agcattccca cggcgcttaa aaagctgcgg gagccgccta
      241 ccggcagaat gacgggctcg atcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nacggctgtt ttcctccgaa tattccatgg
      481 cccggaagcc cagcccgttc agcagccctt ccacagcgcc ggctatttcg tcccgctcgt
      541 cgccgcccag atcgtaaacg gcctccatca ggtcggcaag acgctccgcc atggcggaac
      601 tgccctgctc ccggccccgc agactgtcgt tgagataggc aaagtcgttg gcgcagcggt
      661 cgatagcgcg taactgcccg tccagaacgg ccagcagccg ttcaacatcc ggctgaaggg
      721 tcacgcgcac ctcgtcgtgc ccggtggctt ttcggctttt ccggcgctcg gtcagcaatg
      781 ccgccgaacc ggcgatcagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnncccaac acgatgccgc ggaaatagca gcacagcccg ctgaagatca tcaaaagcgc
      961 cgtcagcacc accagccaag ccttgccacc ctgcttttca gtcttccggg actgcgccac
     1021 ccatgaggtg gcagccaccg gcgtttccaa aaagcccaca gaacccttca ccagactgaa
     1081 cagcactccc atctgctgcc ggagcacttc gtcgctctgt tgagccagcg tgttctgctc
     1141 ggtctgcatc aaagcgtggc ggatggcata cacctgatcg gctacgctgg tctggtcttt
     1201 cagcgccgcc cgcagggatt cccgctgctc ttcccagcat tcaaaggcgc tcatgggttc
     1261 gttcccttct gggacttctt tgcggacatg gacttagcgg tggaagccgt ctttttcgcg
     1321 gtcgaagtct tggcagcgga agtggtcttc tttgcggacg tagactttgc ggcagaagtc
     1381 gtcttcttcg cagacgagga cttcgcggca gaagtcgccg tattcttcgc ggacgtggat
     1441 ttagctgcag tagtcgtggt tttcttcgtt gccgtggact ttgcagcaga ggtggttttc
     1501 ttcgctgtcg tagacttcgc agcggaagtg gtctttttcg cagctgtgga cttagcagca
     1561 gaagacgttt tcttcgttac cgcggatttc gtggcggaag ccgtcttttt cgcagtcgaa
     1621 gtcttggcgg cagaggtggt tttcttcgct ggcgtagact tcacagcaga ggtggtcttc
     1681 tttgacgtcg caggctttgc gtcagaagtc gtcttcttcg cagccgtgga cttcgcagca
     1741 gaaggattct ttttcgccgt tgtggatttt gccgtggtcg aagctttctt cgtcgttgtt
     1801 tttttgctgg aagaagtctt cttctgcttc gtttcctcgg caccggtcag cccggacagc
     1861 acattgccaa tgccctccaa cagcccgccg gaggacgcag cgttccccgc cagatcggag
     1921 agcgcgtcct cgatggtatc cagaaaatcg ccggtggaga cggagccttc gcccagcata
     1981 tcgcttacga tggattccag cagtcccttt ttggccatga cgaacccctt cttaaaattg
     2041 atcttaaagt tttttcaaag cgtccagttc ttcctgcgtc agcggccgcc acatgccccg
     2101 gggcaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnngggc ggtcttgcgt ccgtccacca tcacgcctcg ttccagctgc
     2281 cgggcttcct ccggcgtgac ctgattgctg acccgcgcca gataggtctt gtccacctcc
     2341 atggagggat gcagcatccg ctcggtcagg tcgccgtcgt tggtgagcag cagcagcccc
     2401 tcgctgtcgt agtccagccg ccccaccggg tacagccgga cgggataatc ccggaagcga
     2461 tccagcaccg tggcgcgtcc ctccgggtcg ctgacggtcg ttacctcgcc agcgggcttg
     2521 tgatatatga tataatgctt ttccacttcg ggccggacgc gcttcccgtc cacgcagatg
     2581 acctgatcgt cctccacctg cacgcccatt tccgtaattt tctgaccgtc taccgtgacg
     2641 tggccctcgg cgatcagctt ttccgccgcc cggcgggaag ccactccgca ggaggccaga
     2701 tacttttgca gccgcatggc gttcctccct gtgcgggctt ggcccgctgg aaaatagtat
     2761 agcggaatct ttccctaaaa acaagacccg gcccgcctgt cacggcgaac cgggaatgaa
     2821 aacgttgttt acagccgctc cacgtggtcg ctgtccacaa aggcgtggat cagcttgcac
     2881 cggggaacgg gcgtggggga gagcgtcaga caggccagaa tttcgctggc cgctcgggcg
     2941 gcgctttcct cgctggaggc atgaacggtc gcgactgcct cgccggggcg aagctcgtcg
     3001 ccgatgcgct tgtgcatcac gaaaccaacg ctgtagtcga tggggtcggt cttctgcttg
     3061 cggcccgcgc ccatggcctg agccgtgttg cccagacgga tggtatccat gctccggatg
     3121 tagccgccct ccacggcagg aacgtcccgg atgatcttgg cctggggcag ctggctcacg
     3181 tcatcgcagc aggcggggtc gccgccctgc gccacaatca tctgccgcag cttttccaga
     3241 cccgcgccgc tgtgaagcgc cgccatcagc ttgtcccggc cctcggctac ggaggacgca
     3301 gcgccgccca gcagcagcat ccgcgcgcca agctccactg ccacggtcag cagcgggcct
     3361 tcgacccggt tgttcagcac atcgatggct tcttttactt ccagagcgtt gccgatgtga
     3421 ctgcccagcg gttcgcccat gtcggtcacc agcgccacca tcctgcggcc cgcgtcgcag
     3481 ccgatacgaa ccatggtctg ggccagctcg atgctggctt cggtggtgtg catcagcgcg
     3541 cccgcgccgg tctttacgtc cagcacgatg gcctgtgcgc cgctggccag ctttttggag
     3601 acgatgctgg aagcgatcag cggcagactg tccaccgtag ccgtcacgtc ccgcagggcg
     3661 tacagggtct tgtcggcagg ggccagattc ttcgtctggc caatgatggc gatgccgatg
     3721 tcctgcacct gccggataaa ttcttcttcg ctcagtccga tgcggaagcc ggagatgctt
     3781 tccagcttat ccagcgtacc gccggtatgt cccaaacccc ggccgctcat cttggccacg
     3841 ggcacgccgc aggcggccac cagcggggcc agcaccagcg aggtggtgtc gcccacgccg
     3901 ccggtggagt gcttgtccgt gggaacgccc ttcagcatgg acagatccat catatcgccg
     3961 gaatgagcca tcgacagagt cagccgcacc gtttcttccc ggttcatgcc gttcagacgg
     4021 atagccatca gcagcgcgga aagctggtaa tccgggatgg agccgtctgc cgcgcccttg
     4081 acaaaatagt caatttcttc cgcggaaaga gcctggccga atttcttttt ttcaatgatc
     4141 tccaccatgt tcatcggaag tacctcacag ttggatcagc attctgccgc agttttcgca
     4201 ttcgatcatc ttcgcgccgc tcttgaaggc gcggagggag gcctgcggaa tattcatgtt
     4261 gcagccgccg cactggtcgc cgtacaactt ggcgataggg cgggcgcaat gctgcttgat
     4321 ggcacgatac ttggccagca gctccgagtc gatgccctcg gtcttttttt cggcctcggc
     4381 gcgcttctgc tccagtccct tcatctgctc cttgtagagc gcctcgtatt ccaccttctg
     4441 cttgtcgtag gcggccttca ccttggcgta cttgacccgg atatcctttt ccatccggtc
     4501 gcgatccgcc gcgtccttct ggatgcgctt gagctcctgc tcgtactcgg tcaggtcgcc
     4561 cagcagcttc tgcgcgtccc ggctcatatc ctgcgccaga ttcagatcgg tgggcggcgt
     4621 ggcttccatc tttttggtca gcgcggtcag ctgctcctcc atccgggcaa tggcgacctt
     4681 gatcacgtcc acccggtcca ccatctgagc cacttcttct tccatctttt tgacggcgtt
     4741 ctgctgctcc accaaaaatt cccggttctt cttcagcgcc aaccgggcgg gatcgcgctt
     4801 gatctcggtt tccagcgcat caacggccac gtcggcctgc tgatactgcc aaagcatttc
     4861 catctgttcc ataaggcaac ctcctttaag gttctgggag aaaacgggtc agcggacagg
     4921 ctccttgggg aaa
//
LOCUS       DAWAEA010000057         4925 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2700_length_4925_cov_2.896304, whole genome shotgun sequence.
ACCESSION   DAWAEA010000057 DAWAEA010000000
VERSION     DAWAEA010000057.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4925)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2700_length_4925_cov_2.896304"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4204..4213
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          930 a         1614 c         1478 g          893 t
ORIGIN      
        1 taggccagaa tgacccggcc gttgagatag aaccggatgc cggtcaggct gcgatcgtcc
       61 gccgcttttt tcaaaagcag ctgggaaaag gccgatccca gcggggtaat gagatagatc
      121 aatacgcctt taagcatggt cggtcaccac cagccatacg cccagcagca ctaccagaat
      181 gcccgccgcc tttcccaaag tcaggctttc gccaaagaat aaaatgccga acagacaggt
      241 ccacagggtg cagacgcctt tgttggaata ggcaaagttc agcggcatcc gttttaaaac
      301 ctgctgccac aggatgctgt acgcgcccag cgccgccacg ctgaaaaaca gaaagaggag
      361 ggtcatgggc cagttggcct gactcagata gcccgaggcc agcttggtga gcactgtgac
      421 caccgcgtaa agcaacagcg tgccgtgcag cagcagaaaa gcctgcaggg aaaccgaaga
      481 ttttcctttt atttctttcg attctttcaa tcaggcttcc tccttttgaa gatttgaaac
      541 cgggagcgag accgccagct cccgcagcaa gccaagcgtg ggactgtcgc ccagacggca
      601 ggacacgggg ggaagcagct cgtagaaaaa gggcgactcc gtccagcgga ccagggaaag
      661 ctccccgaag gcggtaaagg ccgtgcgcac ctgtacgggc gtgaggcccg actgctgcgc
      721 cgcttccgca acgctgcggc acagggtccg ccgaatcagc cggtatagtc cccggcagct
      781 ttcgtcgtcc ggggcgatag ttcctgccag cgccgaaagc gcccggctcc ggggcagaac
      841 gtgaagctcg gcctccggca gacggcgctg ccagagcacc ccctcgccgg ggaataactc
      901 gccgtccagc agccagacct gcgcgtaatg gccgctgacc agctccaacg ccggggcggt
      961 cagcagcgtg tgaaaactgc gggggtctga cggcgcggaa accgctgtgt cccaatccgc
     1021 cagacgcagc gctgccagcg ctgaggcatg ggtgcggctg accgccagaa tgccccgatc
     1081 cagccgggac agggcttctt tcagcccgtc cagcgtgtcg aaggcttcct tcaccggggg
     1141 cgtcccctcc tgcgccgccg ccagacacag cccggacttg acggcgttgt acagagccag
     1201 ccgctccggc tcgccgtccg gggcagcaaa ttgctcctga acggattcct gcaccggcag
     1261 gagcgcctga acttccgcct gtaaggaagt aacgccccgg aaggtgttct ttcccagcgc
     1321 gaagaccgcg tccacccgtt ccggcagtgc gctggccttg ctgcccatgg aaaaggcaat
     1381 gcccggcagg agttttcctt cctgccgcag ggagagcttc agatgagcgc cctccgcgcc
     1441 tacagcccgc cgttcctcca gcgccgcact ccggcacaga agcaggggcg cgggattttc
     1501 acagccgaag ggagccagca ggttcagctc gtcatagagg gcatcggtgc attcgtccag
     1561 ccgaatctcc gcgtcgtaga gctgggcagg caccagacag gccgggtcgg ccttgcgtac
     1621 ctccgcttgc aggcgggcgc ggaactcatc ctccttgtcc gccgataagg ttacgcccgc
     1681 cgcctgctca tggccgccgt agcgcaggag aatatcgtcc agcgcctgta ggcaatgatg
     1741 aatgtgaatg cccggcacgc tgcgcagact gccgtgcaaa agcccgtttt cccggctcag
     1801 gacgcagacc gggcagtgat actgattgca cagccgcccg gcggccaagc cgatcacgcc
     1861 tacgtgccat tcgtccccgg aaacgatcag agccggttct tccagaaaat tatgatcgag
     1921 ggccgcttcc tgcgcttgac gcaccagacg gctctcggcg ctcttgcgcc ggtcgttgag
     1981 ggcgttcagc tccgccgcca gactttccgc ctccgagggg gaagccgtca tcatcagacg
     2041 cacccctttg cccgcatcgt ccagccgccc ggcagcattc agccggggcc ccagctggaa
     2101 ccccagcgtg tccgaggtca ctccgccgcc gcaggcgctc acgcttaaaa gcgccttgat
     2161 ccccggacgg cgggcctcct gaatggcttt cagtcccagc gagaccagca cccggttttc
     2221 cccggtcagg ggcacgatgt ccgccaccgt ggccagcgcc gccaagtcca gatattcccg
     2281 gcaggcttcc agccccagaa gggcctgcgc caccttgaac gccacgcccg tgccgcacag
     2341 gcggcggtag ggatagctgc ccagcagcgg gttcagcacc gcgtcgccgg gactttccgt
     2401 caggggcaga ccgtggtggt cggtgacgat caccttcatg cccagctctt tggccagcag
     2461 gacttcctcg tgattggtaa tgcccaggtc caccgtcacc agcagccggt atttttccgc
     2521 cagcttccgc accgcgtcct tgttcagccc atacccctcg gcccggaggg gcgtgtgggg
     2581 agcggcgacg attccgtacc ggcgcagagc ctgcgtcatc agcgaggccg cgcagatgcc
     2641 gtctacgtcg tagtcgccgt aaaccaccgt gggaattttt tcgtcccggg cctgacggag
     2701 cagcgcgacc gccttgtcca tgtcctgcat cgccatgggg tcgtgcagct gatccaagga
     2761 gggatgcaga aagacctccg cttcggcagc ggtctcgata ccgcgggcgt aaagaagctg
     2821 cgccgccgtg ccgcgatagg gcagcagcgc agccgccgcc tgcggggtca gcgggcgggt
     2881 gtttcgggga agaagacgaa gcaagggaac ctcccatccc gccagagggc ggaaacatca
     2941 tttcaaaacc caaacgggtc agccgctttt tgcaaggcac tttaccctgg ggagaaggca
     3001 tacaaggttc atcgtatcct tcccgcacgc catccagcat gcagcgtttt caccctgcca
     3061 gaccggcgag ccgcgaggcc tgtcgccgag cggcggtaac tccgcagcca agccgttttc
     3121 cctcgagtac ccccgcgccc cacgggcgcg gtgtggggtg cagggggaca ccccctgccg
     3181 cgagccgcga ggcccgtcgc cgagcggcgg ccttggggac gaaagcgtcg ggaacgcgag
     3241 gccctctgcc gagcgttccc aaagcgccat acttacctgt atagccctgc gcgcctgcgg
     3301 cgcgcagtcc ggggttaagg ggcactgccc cttactggtt gaggggctgg cgggcgatgg
     3361 tgtcttccat gatctgcttg cgttcatcgt cgctgatgcc ggccagaatt gcgttgatgg
     3421 aatccatcag ggggctgccc ttggccagac cgaccgcgat ggacgtttcg ccttccgcca
     3481 cggtgaagcc ttcgccctcg tcaaaggaca gataggtcaa ttccggattg gccatagtgt
     3541 ccgccatggc tccggggcct tcggcaatgt agccgtccac cgcgcccgcc ttcagcgcca
     3601 cgatcatggc cgggaaggtt tccatggcca tggccttgtt cacgccgggg atctggtcga
     3661 tcaggccgta gtggaaggtg ttcagctggc cggtgatact ggccccgctc agatcagcca
     3721 gcgtcttggc attggcatag gggccgtcct tgcgaacgac cacaacgggc tgcacgttgt
     3781 agtacgcgtc ggtaaagtca atggtggcct tccgttcttc agtggggctc ataccggcga
     3841 tgatcgcgtc gaacgcgccg gtcatcacgc ccatgggcag accgtcccat tcggtcttga
     3901 cgatcaccag ctccttgccc agcccttcgg cgatcttccg agcgatctgc acatcgtagc
     3961 catcggcgta tccgccgccg ccttcgatct caacggcata ctcgctggca ttggcctgcg
     4021 tccagttgta gggggcatag ttgcactcca tgcctacgcg gaaggtgcct tcggcaaagg
     4081 cgctggcgca gcacagcatc atcatggcgg caaggaacag ggcaaggatc tttctcatcg
     4141 tggtttcctc cttcaaaaaa tggggaatga aaaaagcagg aacggcgcaa cgccattcct
     4201 gctnnnnnnn nnngcagaaa aacggtcgaa tagcgcccca cagccacttt tggctgtgac
     4261 agtcgacgaa atgttattcc gtcgcccaac ggacgcgctc acacccgaac gcccgtttcg
     4321 gcggccagcc ctttcgcgcg cggtcacagc ccggtgaggg gctcccgcgc ggtactgatg
     4381 cagtccgcgc ctctattccc ttccagcttg gccggaagtt atgcctattg taccccacaa
     4441 tttttgtttg tcaaggggcg aaaccacaag tacaggaaaa atacacccta cacccgcagg
     4501 gccatctcga tctcgccgtc ctcgtcctcc gcgccggtgg gcgcaaagcc gtagctttcg
     4561 tacaggcgca gcgccggaag gttttccttg tggcaggtca gcttcaccag atcagaatcc
     4621 ccgaagggct tctggcggat gtagttcaga aacagtccta ccgccgtttt gccgtacccc
     4681 ttgccctgct gctccgcgtc gatcatcacg tgccagaggt cgtactcctc ctcgtcgtaa
     4741 tcgtagatca ccatgccgta gcccaccatt tcgccgtcgg ccaccacgcc gaagggcgtg
     4801 cactgcttgt aatacacgta cgcctgcgcc agactgcgga tgggatggga aacgaagcgc
     4861 tcctgttctt ttttcagttt caggttgaag cagtcgatga agttgctttc gtcaatgcgc
     4921 cgaag
//
LOCUS       DAWAEA010000058         4894 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2720_length_4894_cov_1.856375, whole genome shotgun sequence.
ACCESSION   DAWAEA010000058 DAWAEA010000000
VERSION     DAWAEA010000058.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4894)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2720_length_4894_cov_1.856375"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    209..423
                     /estimated_length=215
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          893 a         1338 c         1549 g          899 t
ORIGIN      
        1 cgctgttcta cgccctgcaa aatctggaca cgtcccggct gtcggtcatc acctgcgacc
       61 cggcggcgct gcgccgcctt accggcacag acgtcagcgg cgcgccggtg gattatctgg
      121 aagaaattct gcaggaacgc ggcgtattcg atcagggcct catggtggga ctgtacgtga
      181 tgcagctgga ttacatgggg caaatgacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnggatgcg gacacgtctt tgcagggaac ccgcggcccg gaaaaggggc agctttgcta
      481 cgcggtccat ggagaaacag tgacgcagga gccgttttcg ttcttctatg aatacacgga
      541 aattcagcgc ccctcggcgg ggctgtacac acagcgggcg gacggcttca ctgtggagac
      601 ggagcagggg acggtcagcc gggaaaatgg cctgtacacc gtgcgactga caggccgggt
      661 gggcaacgaa gggatcacgc tccattggcc ccttcaggca gtcgagggcg cggaaacgct
      721 ggatttgtcc caactgacgg ggacgagcgt ccgggcgatc gattcgctgt tgctggattc
      781 cgttctgccc aacaccccgg aaacggcgga ggacagccgg gaagaacgtc aggttttgac
      841 cctgcgggac aaacgatacg ttttcgttcg gcaggacgat cccttcgccg atggaaagga
      901 agaaaatccc tttacattga cagatattca ctggaatgaa gaaaacggac agctggaagt
      961 aaatcttcag gtggatcata cgaagctaaa aagcggctat tatcgacttg aagtggaagc
     1021 aaacctatct ggacgacaaa ttctatggcc aaaagtggac tggatcgacc aactgggcac
     1081 ggagatcacc aacagtcaga tcagtacgtg ggaagccttc acccagcatt tgtaccgcta
     1141 cggcaggcgg ctgaaaaacg tgcccaagca gtttgaccac gcgtggggcc cggccacctc
     1201 caagccttat caggacgtgg caattcccga cttcccgccg gtgtaccggg cgctgcatct
     1261 gcaaagtctg gtgcagcagc ttcgggaagg ggccaatgtg gaaaacgttc cgctgatccg
     1321 ctgcgttttt gacgtattcg tggccgatat gcccgaaccg tagggcaaag gagaatgatg
     1381 atgggaaaag gaagaagccg gcttcgctat tccaaccgaa cgaaggtgct gctgtgctat
     1441 ctggcggcgt tcgggctgcc ggtgctttgg atgctggcgg ggctgttttg gggatatccc
     1501 tataagctgg cgggtagcgc cccccatatc gccgagacct tattggggct gttccagggg
     1561 caggcgaggc tcgcgaccgc cgtatccgcc gcccagacgt ctgcgggagc gtcgccggag
     1621 ctctggcggc aggccttgca gcaccgggaa cagcaatggc tgctgttttg gacggcggtt
     1681 tgggccgttg tctggctgct gacgctgctt ttgcagctgg gctggcggct tcggcatcag
     1741 aagccctgct tcgccgcccg gaacaccctt cgcgccattc gagattaccg gctgacccag
     1801 actgtgatct gggcggtgaa tctggcgggc ggggcggcgc tgtggctgct gggcgtgcag
     1861 ttcgtgcagg gccgcaccgg ctgggactat gtcatttatt tctgtccttt tctgctgaac
     1921 ggtctggcgg ctatcgtctg ttttcggctg gccgcgccgc cggtgctgag cgggaaggga
     1981 gcgttcttca agcgtctgta ggaaaggggg atgcgccatg ggctattatg tgctggcggt
     2041 aggcggcacc gggaacaaaa ttctggaaag cctggtctat ctggcggcag tggacgggct
     2101 gtatacgcag gacgagtccg ggaaaacggc gccgctcccg gagatcacca tgctgtccgt
     2161 ggacgtggac acggcctgcg gcaacaccac acgggccaag cgggccgcgg aaagctacgg
     2221 tgagctgcag cgggcgtttc ggcaatatcc ggcggagcat cccggctttc acacggcgct
     2281 gcaactggag cgttggagca tgaacctgtc ccgccgggcc atgtccgtgg ataaaatggt
     2341 ggaaaatcat cgccgggatc agctcctgag ccgcaccctt ttcagccgca cggaatccgc
     2401 gctggagtac agcgagggct ttcgcggcca tcccgatctg ggggtgctgt tttttcacga
     2461 cctgctgaac tcgctggacg atctgcgccg ggaaggtcag ccggacgaaa tgaaccgcct
     2521 gctggatcaa attcaggcgg agctggatcg ggacgagccg gtgaagctga tcctgtgcgg
     2581 cagcatcttc ggcggcacgg gcgcgtcggg cattcccgcc gtggcgcggt tcctgcgcaa
     2641 gcgcttcgtc gcccgcagcg accgttttga aatgggcgcg ctgctgatgc tgccctatta
     2701 ccgggtgccc gcttctctgg cggacgagga caccgaaatt gtggtgaaaa gcgacgattt
     2761 tttcgacaag gcccgcacgg ctctgcaata ctacggcatg gagggcatga tccgggacgg
     2821 ggagcaggac gaaaacggcg tgttcgacgc gctgtacctg ctgggccttc ccccggaggg
     2881 cttcgtgacc acctgccgct actccaccgg ctcccaaagt caggaaaacg acgcgcatat
     2941 gctggaatgg ctggcttccc gctgcatcgc aaagtttttc cgcaccgggt ttcggggcgg
     3001 ggaacagcat cacatggact gctactatta ccagctgcat acaccgtcct tctgctggca
     3061 gagcttcgat cgggaggaga cgctgtaccg ccggggctat ggctcgctgc tgaaggcggc
     3121 agcgttgttc ttcgccgagt gctaccccac gctgaaaagc tgcgtttcgc tggacgatcg
     3181 tcaaagcgcc cgggtcaact actgcgcgcc ctattttgcc catcgccctg catcggagcg
     3241 ggaaaagctg gaaggacttc tgaaaacgct gtataccttt ttgaatttct acgccaactg
     3301 gatgatccag atcgtgcgca ccctgccgcc cattctttgt cagcccgcag aggcagaggg
     3361 ccatttgccg gaaaacgacc tgctggatgg agaagggatg gacgagctgc ttctgctgct
     3421 gacgcagtat ggccttcgca cggaacagcg catccgcgct gaaatgagcc gggcggcgga
     3481 aaagctccag cgggagctgc atcatctggt cgtccgccgg gtgcccgacc gccagacggc
     3541 ggcgaagatc atcgcagggc tgggtggcgg tcaacggctg ggagccgggg cggagggggc
     3601 cgtctgctcc tttatggcca cgcttcttcg ctgcgccgga gaggaggaag accgatgaga
     3661 gacgaagctg aaaagcgccg gttggatcgc caaacgccct atttgagcct gctgcccgaa
     3721 atggacgagg ggatgcacat cggctatcag gaaaaggcgg gcatccaatg cgatggcaat
     3781 cagaagccgc gggaatggct ggaaaatttt gcccgttttc tgaaaacgcc gggccgggag
     3841 gtgggctatg ccatcccgga cgtgctggcc agcgaacaac agcttcgccg tctggcccgt
     3901 ctgccgtggg aacaggccat gacccatcag gaaatgcgcg attaccgggg cattctggcc
     3961 ctgctattgt tgtgggatct gttggccgag gacggctcct gtgcggagct gcggctgtat
     4021 accatggagc cggacgaacg tggcggtttt gtccgggccg tgggggaggc tctttccccg
     4081 gcgcgcgccc gggaagggct gcaggtattc accctttgcc gcacccagtc ggagcatccc
     4141 gagccggttc cgctgtgcct tttgtcccat tccatggtgc tgatgcccgc cgccaatctg
     4201 ggcgacctaa gccgactgct gccgccatcc gttcgctggt acgaccggga aaaacgctgt
     4261 tttctcgacc cctgcggcta tctggacgag cggatgatct ccgtgctggt gccccggctt
     4321 cgtctgctgg cggcgctgaa cgaggacgtt tccgcccaca gcccgattta ttccccggac
     4381 gcgccccttt gcgctttgct gaatcggatg atccgggaga tcctgcaaag ccgggaaaac
     4441 ggccggcagc ggctgaacgc gggagacgaa aatgccgtcc gtgcgttccg cacgagggtc
     4501 atggccgttt atgggctggg ccgtcaggag acgggcctgc ggctgacccg ggagcacctg
     4561 ccctgtcagc tgaacggctg ggaaaagaat cccctgctga agcagctggt caatgcggag
     4621 taccccctgc cggagctccc ggagggggaa gaagtccagc tttatttgct ggacggcgcg
     4681 gcctttgccc gccagtccga cgcggcgctg ctggaacctg ccggaacgga aggggaagat
     4741 gcgctgcttc gccgtatcgg agaggaaatc gccctgctgg atcggtggga cgagggctgg
     4801 cagcggcaga tgagcctgac ccttgcggaa ttggccgatg agatagaaaa tcacaccggc
     4861 ctgtgcccct gcgttcccgg gctgctgcgg cagt
//
LOCUS       DAWAEA010000059         4869 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2735_length_4869_cov_2.878272, whole genome shotgun sequence.
ACCESSION   DAWAEA010000059 DAWAEA010000000
VERSION     DAWAEA010000059.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2735_length_4869_cov_2.878272"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          888 a         1516 c         1542 g          923 t
ORIGIN      
        1 ctgcgcgcat agcgcgcagt ccggggtcaa ggggcactgc cccttgcgcg agccgagcgg
       61 cggtaaaacc ccggccaaga tgcatgtcgc cctacgcccc tgcgcgcaca gcgcgcagag
      121 atgggtgcag ggctttagcc ctgctggtgg ttgcggttag ggccgcggag gaggtcgccg
      181 ggggcgacgg ggaagggaaa gcgcagacgg agcagcgttc cggctacgct ggccgtctgg
      241 attgactcgc ccgtctcggc cagaaccatg gagccgaccg tgaagggatg actgcctgtg
      301 ggggtcaggg cttccagctc gtcgcccacg tagaagcggt tcttcaggcg gatcagcgcc
      361 ggttcgccgg gcgcggcggc ttccagtacg tcgcccacgt attccatggt ctgggaaaag
      421 ccctccgccc cggcgacggg ctggggcgcg ccaaagtaga agcccgtgtt gctctgccgg
      481 tggctggctt tgttcagctc ccgcatcagg tcgggcacgg cccggcggaa ggcctcctcg
      541 ccctccagct ccagtagatc cagcgcccgg cggtagaccg ataccaccga cgctacatag
      601 taggccgtct tcatccggcc ttcgattttc aggctgataa cgcccgcgtc ccgcagctcc
      661 ggcagatggg gcagcataca aagatcgtag gccgagtaga gataggttcc ccggtcgtcc
      721 tcgcagacgg gcaggtattc gcccggacgc ttttcttcca tcagatgata ctgccagcgg
      781 cagggctggg cacaggcgcc ccggttgccg ctgcggccca ccagtgcgtt ggataacaga
      841 caccgcccgc tgtaggccac gcacatagcc ccgtggacaa aggcctccag ctccagctcc
      901 gacggcagga gggcgcgcat tccccggatg tcctccatgg acagttcccg ggacaggacg
      961 atccgctctg cccccacggc ccggtgcagg tgcaacgccg tgggcgcgtt catcacatta
     1021 gcctgcgtgg agatgtggat gggcagcccc ggcacccgct catgcagggt caggcaggct
     1081 cccagatcgc tgacgatggc cgcgtccgcg ccgcagcgca gagcggtctt gcccgcctga
     1141 gcaaagccgt ccagctccct gtctgtgggc aggatattca tggtgacgta gaatttcgcg
     1201 ccctgctggt ggcattgctc cgctgcttcc gcaagggccg cttcgtcaaa attgcccgca
     1261 aaggcccgca ggccgtactg cttcatgccg ccgtataccg cgtccgcccc gaagcgcagg
     1321 gccgccttca gctgctccat gcccccggcc ggggccagca gctccattct tttgcgttgc
     1381 ttcaaagtca aattccccgt tcctgatcgt aggcctgcca caggggcgag tagatggcca
     1441 cggcctgatt gtgctcctcc agcgtcttgg agaaatacag ctcgccggtg ctgggatcct
     1501 tgctgcagaa gtagaggtac ttctccgcca cgtagctttc gtccgggtac agggccgcgt
     1561 aaatggcctc gggggacggg ttgcagatgg ggcccagcgg cagaccgctg acaatgtagg
     1621 tgttgtaggg tgaatccacc tgcaggtcgc tggagcgaag ggccatgcgg cgttcgcctg
     1681 tgatatagtg aatagtgggg tcgctctgca gcttcatgcc gctgttcagg cggttatgga
     1741 agacggcgga caccttggca aaatcggcct tcttggcttc tttttcgatc atggaggcca
     1801 gcgtcagggt ctgatccatg gtcatgccca gctcggcggc ccgctcctgc cactcgttgg
     1861 agaaaacata gtccgtctga tccagcagtt ttttcacgat gtccttgggc gtggcgttta
     1921 ggtagacctc ataggtgttg ggggccagat agccttccag caggtatttg cggctgttca
     1981 cgttctgggt cttcagctct tcctgaagga agtaatagtc cgtcacgccc tcgccggtct
     2041 tgcacaggtt caggaattcc gccgtatcct gcaaaatgcc cttttccttc agataattgg
     2101 cgatgttctc gatggtgtag cccggaatga tcgtgatatc cgtcacggtg ggccgtccgt
     2161 cgccggtggt cagaagctgg gcgatctgga acagatccat gctctttttc accttgtaat
     2221 cgcccgcctg gatcttctgg cccattccgg cgaagtcgca gtaatacttg aacacggagc
     2281 tgttcttgat cagtcccgcg ctttccagat tgcgggatac ccggctcagg ctgttgcccg
     2341 tctccacgga gaaggcgatc tccgtatcat cggcagggtc taccgggctt aagtacttgt
     2401 cctgcacgtg gctccacacg gtggacagca cgccgaacac gatcagcagc gcgcacaggc
     2461 cgatcaggac gggccggaaa attttccaca ggccgctgaa ccagtacagg ccgtattttc
     2521 ggtcctcccg cagggattcg tagtcgtaat cctgctgttc ttccggcaat atcggttgat
     2581 tctttctgcg tttcaatggg tttcctcctt gctcagccga ggggaatatc cagcccggcc
     2641 tcccgtgtga ttttttccat cagctgggcc accagctgct gcaccttcat ctgggccagc
     2701 agatattcgc tggcctcctc gctgtcgtat aaaagggcgc tgagctgctc gaaggcgcgg
     2761 gtatcttcct cccggggttc gccgccagaa agggccgcca tctgcaaggc gctctgggcc
     2821 gcggcgaacc ggcgcagcag cgccgccgtg ccttcatcgg ccatcacgct ttcccgcagg
     2881 gccgcgtagc gcctccagct ttccgtctgg tgcagggcgc tcagcagctc gtccgccgcc
     2941 cggcgcaggt tctcgtccat ggtaccgccc tccctgtggc ttgcttccag aaaatcccgc
     3001 ccttagtata acgcatcttg agggaaattt cattccctat tttctccctc cgccggtaac
     3061 atacccgcaa agagccgcgc atatccttgc accagccaga cgaaggagga atgaacgctt
     3121 tgagtgagca gaactttgat ctgtaccgcg atattgccaa acgaaccgac ggcgacgtat
     3181 acatcggcgt ggtcgggccg gtgcgcacgg gcaaaagcac actgatttcc cgaatgatgg
     3241 aggaactggt tctgagccgc atggcggcgg gcccccggcg ggagcggctc accgacgagc
     3301 tgccccagag tggaagcggc aagaccatca tgaccaccca gcccaaattc gtgcccagcg
     3361 aggcggtcag cgtcacgctg gacgatcagt cctccgtccg ggtgcgcatg gtggatagcg
     3421 tgggctatct ggtggagggc gcgctgggca ccgaggagga cggcgcgtcc cggatggtgc
     3481 acaccccatg gttcgactac gacgtcccct ttgagcaggc ggcggagatc ggtacccgaa
     3541 aggtcatcac ggatcacgcc accatcgggc tggtggtcac caccgacggc agcatcgccg
     3601 acctgagccg ggaggcgtac cagccgccgg aggagcgggt ggtgcgggaa ctgaaggaga
     3661 tcggcaagcc ctttatcctg atcctgaaca gccgccaccc ggaggcggag gaaacgctgg
     3721 ccctgcggca acggctcagc gagcgctacg gcgtgcccgc cctttccctg aacgtaaagg
     3781 aaatgggact ccccgaaatt cagcaggtct tcgaagaagt gctttatcaa tttcccctgc
     3841 gggaaattcg cgtttccgtg cccggctggc tcaacgcgct ggacgagggg cactggctgg
     3901 tgcagcacgt gctggagcac gtgaaggaag ccggggcgaa ggtcagctgc atgcgggatc
     3961 agacggtctt tgccggggcc tttgcggaca gtccctacac ggacggtctg cggccggacg
     4021 gcatcgagct gggcaccggg cggctgaact tttccctgcc cctgaaggac ggcctgttca
     4081 accgaatttt aggcgaggaa tgcggcacca ccatcgaggg cgacgcgcat cttcttcggc
     4141 tgatgaagga actggtggcg gccaagagcg agtacgacca cgtggcggac gcgctgcgca
     4201 gcgtccggga gacgggctac gggctggtca cgcccaccat gaacgagctg accctgcagg
     4261 agccggagat cgtccagcag ggcagccgct tcggggtacg gctgaaagcc aacgcgccca
     4321 gcctgcatct gatccgggtg gacatcgaaa cggaggtctc ccccgtcgtc ggcacggaaa
     4381 agcagagcga ggaactggta aagtatctat tggaggaatt ccagaacgat ccccagacca
     4441 tttggaacac gaacttcttc ggcaagagtc tacacgaact ggtccgggag gggcttgcca
     4501 acaagctgat gcggatgccc atagacgccc aagagaaagt acaggagact ctcagcaaga
     4561 tcatcaacga aggcaacggc ggcatgatct gcatcctcct ctaacaaggg gcagtgcccc
     4621 ttgaccccgg actgcgcgct gtgcgcgcag gggcgtaggg cgacatgcag cttggccggg
     4681 gttttaccgc cgctcggcga caggcctcgc ggctcgcgca aggggcagtg ccccttgacc
     4741 ccggactgcg cgctatgcgc gcagagcgct cggaggaaag cagattggct cctttctacc
     4801 gccgctcggc ggagggcctc gcggctcgcg ggtctggtgc agattcccgc cagctgcaca
     4861 gcaaagacc
//
LOCUS       DAWAEA010000060         4836 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2759_length_4836_cov_1.702364, whole genome shotgun sequence.
ACCESSION   DAWAEA010000060 DAWAEA010000000
VERSION     DAWAEA010000060.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2759_length_4836_cov_1.702364"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    812..973
                     /estimated_length=162
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4388..4578
                     /estimated_length=191
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          953 a         1354 c         1410 g          766 t
ORIGIN      
        1 catggtcatc gatcaggagg ccacggacaa gctgcggcag gaagcccggg acgcggtgga
       61 gcccgtggtg tacaagcagg ggcagaacat cgtgctggcc cgggagcggg tgacggccaa
      121 tcagctggaa atgctgcgct cgctgggaat gctggataac gacaccttcg acctgaacat
      181 ttatatcggc gcgttcctgc tggtgctgct gagcatgatg gtgctgctca tcgccctgat
      241 tttgctggat ccaaccccta tctgaccccg gcactgctgg gcgtgatgat gctcaccggc
      301 ctgatcggcg cccgggcggg cgtggccggg tgtctcaccc tgacggtgct ggtcagcgcg
      361 ctgctggcgg gcggcgatac cagctatact tcggagatcg tgaacctttt gttcatttcg
      421 tttatctccg gctttctggt ggtcatcatt cttcatcgga agccccaccg ccagcaggtg
      481 ctgctgtgcg gcttcctgac cggcctttcc aatatggtca tcatcctcac catcggcttt
      541 atgaccaaca actccatggg ggtggtgatg aacaacgccc tgtggagcgc cctgtccgcc
      601 gtggtggcgt cggtgctgtg catcgcgctg gacgcgctga tggaggcggt gttccgtctg
      661 gcgacccccg ccaagctgct ggagctgtcc aaccccaatc agcccattct gcgccggctg
      721 atgatcgaag cctccggcac ctatcatcac tccatcatcg tggctaatct ggccgaggcg
      781 gcggcggaaa cggtgggggc caatcccctg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnacatcgg caagctgaag cgccccatgt actttaagga aaaccagctg
     1021 ggcgaaaatc ctcatgagca taccaatccc tatatttccg ccgccattgt caccgcccat
     1081 acccgggacg gcgtgatgct ggcccagcag tatcatctgc cgcaggaaat tcaggatatc
     1141 atcatcgagc atcacggcga cacgccggtg atgtacttct accataaggc gctgaaacag
     1201 gcggacgggc aggctgtgga cattgccgat ttccgctacg acggccgccg tccccagtcc
     1261 aaggaaagcg ccatcatcat gctggcggac acggtggagg ccgccgtccg ttccatgccc
     1321 gatcccacgc ctaaagccat cgaggagttt atcgccaagc tggtgcgggg caagatcgac
     1381 gacggtcagc tgaacgacgc gcccctgacg ctgcaggaca tcagcaaaat ttgccgcgcc
     1441 tttacgacgg tgcttaaggg cgtgttccat gagcgcatcg agtacccctc catcagcccg
     1501 gcggcagcgg cccgtctggc ggcccatcag cccaagggcg aggaaggccg ggaggaaagt
     1561 caggtggagg cccgggaact gaaacgggtg acggacgtgg ccgaggccga gcgggaagcg
     1621 gaggaagttc tcagcgagga gagcgtggag ccctccgagc aggagatcga agctgcagag
     1681 gaacaggcgg aaaaggaaga actggaagag tcggagaagt ccgccccggt ggaagaagta
     1741 aaagacggca gggaacggcc gtcttcggag gaacaggatc aatatgagga ttgaatggga
     1801 gtgggaaagt ggaaccgcta cggacgcgcc ctttgcgctg gtggcggact gttgtctggc
     1861 ggcggagggc gtgaagctgc cctgcgcggt acacgtgctg ctgaccgggg atgagcgcat
     1921 tcacgagatc aaccgggagc agcggggcgt agaccggccc acagacgtgc tcagctttcc
     1981 cacggtgcgc tacgctgccg gcaaaaccgc ccgggacagt gaaaagcgcc tgaaagcgga
     2041 atgggacccg gagctgcacg cctgtatgct gggggatatc atcatttccc ggccccgggc
     2101 ggcggagcag gccaaagagt acggccacag cgaacggcgg gaactatgct atctgctggc
     2161 ccacgctctg ttccacctga tgggctatga ccacatggag gaagacgaga agaaggagat
     2221 gcggaaaatg gaagaaaagg ccctgaacat ggccgggatc ggccgggata cggaaacttc
     2281 catcacagac ggcgaactgc tggaactggc gcgtcgggcg ctggaataca gctactcccc
     2341 ctacagccat tacgcggtgg gcgcggcgct gctctgcgcc gacggccggg tctttaccgg
     2401 ctgcaacatc gagaattcct ccttcgggct gaccaactgc gccgaacgca cggcgctgtt
     2461 caaggccgtc agcgagggcg cgcgggagtt caccgccatc gccattgcgt ctaacggcag
     2521 tatgccctat ccctgcggcg cctgccgtca ggctttgaac gaatttgcgc cggagctgcg
     2581 ggttctgacc atcgcgggcg atggaagcac cgacgacacc accctgcggg cgctgctgcc
     2641 tcacggcttc ggccccaaag accttcccga ctaaggataa aaggagttac tatgcagcat
     2701 tctggtttta tcgccattat gggccgcccc aacgtgggca aaagcactct gatgaacgcc
     2761 atggtgggcg aaaaagtggc catcgtctcc aaccggcccc agaccacccg caaccgcatc
     2821 atgggcatct atacggagaa ggacgcgcag atcgtctttc tggacacccc cggcctgcac
     2881 acgccccgca cccgtctggg cgaatacatg atgaaggccg ccaaggacgc gctgcagggc
     2941 attgacgcgc tgatcgtcat ggtggacgct accgccgtgg gcaagcacga cgaccagatc
     3001 gtggaggaca tgagccgcta cgaggtgaaa aaggcgctgg tgctcaacaa gatcgacctg
     3061 ctgcccaagg aatccctgct ggcgctgatg cagcgctatc aggcggcggg gtatgatgac
     3121 atcgtgccca tcagcgccgc caagggagac ggcgtggagg aactcaagca gctgctgatc
     3181 agctacctgc cggaagggcc gcagtacttc ccggctgacg cggtgaccga tcagcccgaa
     3241 cggctgatct gcgcggagct catccgggaa aaggcgctgc tgcacctgcg ggaggaggtg
     3301 ccccacggca tcggcgtgga aatgatggcc atgaacaagc tcagcgacaa tctgatggaa
     3361 atccacgcca ccatttactg cgaacgtgct gcccacaagg gcattatcat cggcaagcag
     3421 ggagccatgc tcaagcagat cggccaggaa gcccgtcagg acatcgagac cctgctggga
     3481 atgcacgtcc acctgcagct ctgggtcaag gggcgggaaa actggcgcaa ccggatggat
     3541 gatttgcgca cgctgggata cgaggataag gaataaaaac aggagccgcc cggataccca
     3601 ggacggctct ttttagtgct ttttacgctt ttgcttcggc tggttttcca tgccgaagct
     3661 tttttcggcc catgtaaaca gcaggggatg cagccccacc agatagaaca tcgccagcgc
     3721 tacgcccagc atcgcgccgg tgttggtgcc cagcaggtct tgcagccagt gcctcacaag
     3781 cgtgtaaaga cccgcaaagc ccaccagcag gccggagagc agtcgcagca gctttacgcc
     3841 ccagccgcaa tccgtggtga agcgcacctt gcggcgctcc aacagccagc cgcacagcat
     3901 tcccagtaca gcaccagccg acaggaaaaa atccttctgc atcttcaccg ggtccaccag
     3961 cagggaaccg tcggcggccc ggtcggcggg atagctcttt gccagcacat accagatgga
     4021 caggccgcaa agggccgcac caatggccgt tatcagcaga tcgcggtttt gaccttcatc
     4081 cgcccagcgc agcagacggg ccagcagaaa ggcgcacaac acgcccgtgg ccgcgccgac
     4141 gagtacgtcc gccgaagaac gcacccgcct tgctggaatg gccactgggg aaggaatacc
     4201 ccgtggcggt ggaaagggct ttctccacag gatggagcgc cggttcccgc acccacgggc
     4261 ggtaaacgca gaagccgagc ttgagcacgt tgttgagaag gtcgctgccg ttgagggtaa
     4321 agagcagccg ttggcccagc tcccggctga agcaccagta gacgatcagc gtaaccgcca
     4381 taggcagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnaa aggctttccc gcaggccttg cagccccagc agatagtgca
     4621 gatccatcgc cgtccccgcc ctttctggtt atttggtgta tttgccccgg atgcgggtct
     4681 ggggagccag ccccggcatg gaagtgatgc ttcggttctt gaccacgttc agcaccagcc
     4741 cgataccgcc catgttggtc actaggttgc tgcctccgta gctgaggaag ggcagcggaa
     4801 tgccggtaac aggcgtcagg cctgtggtca tgccta
//
LOCUS       DAWAEA010000061         4820 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2770_length_4820_cov_2.603358, whole genome shotgun sequence.
ACCESSION   DAWAEA010000061 DAWAEA010000000
VERSION     DAWAEA010000061.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2770_length_4820_cov_2.603358"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4384..4393
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          886 a         1533 c         1423 g          968 t
ORIGIN      
        1 catctccttc tcggcgatca ggtcctcgat gttctccttg gtgcgcatca ggtcttccac
       61 ggacatggtc tcgaaaccgg cggcgggaga cttcacgttc acgccgtggc ggcgcaggaa
      121 atcgtccacc ttgtcctcgt tgtgcttata ggcgcagaaa ccgaccacgc tcacgcccac
      181 accaatcaga atgcccttga ccaacggatt gttattcaac atacaacctt atcctccttg
      241 tcgtttgttt ctttcgggaa agttagcctt tgtgcgccat catttccatg ccctgacgga
      301 acatgctgcc tgcaaaggcc agttcgcttt cgctgcccgc catgggcgtg agagtgagct
      361 gatctttgcc gcgcttgatc aggtagggct gctcccgggg agcccggggg atcagatgac
      421 ggccaaaggt gataatccac gccagcaggc tggcgcgcac cgcgccgggc ttgccgacgg
      481 aattgatgag atagaccacg gacatgacct ccgggtcgaa gctgccgcgc acatggagct
      541 cctgatagcc atgctccacc acggcggcca gcgttgcgcc cgtggacagc cagcgggtga
      601 actgggtcag cgccgagccc gtcagggaca gcgcgccgtc cagcccgatg aagaaaagcg
      661 ccagatagcc gctgggggcc atcgaaaagc ccttttcctc gcaatgcttg atgtgcagaa
      721 gccccgcgtc cgtctgggcg gcataggccg cgccgaggcg gacgatgagc ttttcttcac
      781 tgatcgcgtc agggtcgtat tcgcacagca gggtgcggat acgcgggtta taccggcagc
      841 ttaagacccc cgggacgcgc cggaagggtt cgtccgatgc gactgccgtt tcaaactgca
      901 gccggagccg tccccggatg gcggacagca cttccatatg agaggttccc tgttcgatat
      961 gcattatgcc ctcccatccc cgggcttcat gaacatcagc cgcagggagt tggccaccac
     1021 cagaatggtg gaggaattgt gcagaagcgc gctcatcatg acggaaatac cgctggccgc
     1081 gcccagaatc aggcccacag agttaatggc gatgaccagc gcaaagttct gatgcacgat
     1141 gcgcatggtg gcgcgggaca gccgccgcag agcggggatc atcatggggt cgtcccggtt
     1201 gatgatgacg tcgctggttt ccatggcaat gtccgtggac ttgctgccca tggagatgcc
     1261 cacgtcggcg taggccagcg ctggagcgtc gttgatgccg tcgcccacca tgaccacgcc
     1321 gttgccctgc acctgcagct tcagcacggc ctcggccttc tgctccggca gcagctgggc
     1381 gcgatagcca tccgcgccga cctgctcggc caccgtccgg gcctgcgact ccatgtcgcc
     1441 ggtcagcagc tcgatgtcgc ccacgccgtc gtagcgcagg ctgttgatgg cccggcgcac
     1501 gttctcccgg gggctgtcca tggcgtaaag cacggccacg atctcgctgc ctacgcccac
     1561 atagttgggg atagcgcccg tttcaccggg caggggagcg gcggccttca caccgttttc
     1621 ccgcatgtat tcgatattgc ccacgcggac ggtctttttg cccgccaacg tgcggctgcc
     1681 gcgggacacc tccgtcacca cgtcgccgtg cgccttgggg gacgcgccca gctcccgacc
     1741 gtagcgcacg atagctccgg ccaacggatg ggtgctggtc tcttccgccg ccatggcgta
     1801 ggagagcact tctttctcgc tcatgccctc tttgaggaca acggcggaaa cgatcctcgg
     1861 ccggccctcg gtaatggtgc cggtcttgtc cagcagcacc gtgttggcgc tgctcatctg
     1921 ctcgatgaac gtgccgccct tgatgagcac gccctgcttc accgccgtgt tgatggccgc
     1981 ggagaaggcc gttgccgtgg acagcttgat gccgcaggag tagtcgatga ccagcatctt
     2041 cagcgccttc tgggggttct tcgtcaccag ccacaccgac aggcagagca ggaagttcag
     2101 cggcaccaga tagttggaga atttgtccgc gtagcgctgg atgggcgctt ttttcagttc
     2161 gctggcctcc accatgccca cgatgcggga aatgacggtc tggtcgccga ccttggcggc
     2221 ctgcacgtga atgttgccgc tcttgaccac cgtgccggcg aatacttcgt cgccttcccg
     2281 cttctccacg ggcacaaatt cgcccgtaat ggggctctgg tctaccactg ccgcgccgta
     2341 gacgactttg ccgtctaccg agatcttttc gcccgtgtga accgccaccg tgtcgccgag
     2401 gctaatctgg ttcatgggct tgcgctccag cttgccgtca ggcatgacct tccacgcgtc
     2461 gccctcgtcg gcggagagca tctttttgat ggaggaacgg gtgcgctcga tggtgtagga
     2521 ggtcatcagt tccgccagat cggacagcgc caggatcatc agcgccgagt gagcgttgcc
     2581 cagcagcaga ctggccacga tggacgtgac cgtcaggaaa tccgcgttgg ggcgcagatc
     2641 ccggcgcagc ccttcccacg cgcttttcgc catgggggcg gacaagccca gactggcaag
     2701 ggcttccagt gaagtgaagt tcgccagcgc gccgcccatc aaaggcgcgg tcagcagggt
     2761 gttgcccagc accagcgccc cggcgttcac ggccagccgc ttggccagac gggaagtggt
     2821 catgggcgtt tccgccgctt cctcaccgct ttcagcgtgg agctggcgca aggccgccat
     2881 gctgtgacgg gccagtacca gatcggcctg ctcggacagt tcggcggact ccaccatgcc
     2941 gcggtcatac tgcaaaagga tgccgccggt gcagacggtc acccgcgcca gctgcacgcc
     3001 gggaatggaa gccagctccg cggcaatagc cgagctttct tcctttagat aacggagccc
     3061 ctccgcggtc agccgcaacc gtcccggcag actgtgggcc acgcgcgttt tcataaaatc
     3121 ttcctgatgt tctttccgaa gcatattatc ccttctttgg ctgcgggagc cgcaggatca
     3181 gccgctgacg gacgatctct tccagctcct tttcgccggc gttctgccag acgtcttctt
     3241 tcgaaatgca aatgccctcg tctaccacga tatccaccca gcgcagaatg gcgcgggtgt
     3301 tggtcacagc cgcgtcatag cggatgagca ccgttccgat gcggggattg accttcgccc
     3361 ccgatacgcc gggcagcagc tgaagcacgt cctgaatata gtggagatag ggcagcgcct
     3421 ctttgggcag cagatgatat ttggaaaaac tgatccgcag ccgccccggc aggtcgcatt
     3481 ccaccttggg gtgcaggcac agccgcagga agctttcttc cattttattc attgctttgc
     3541 gctccaaaca gcttggaggc ccaccacagc agcgtcatgg ctcccggcgc ggcccagccg
     3601 tatttcgtct tgctgcgcag ggcagccacg gtcagcgcgc cagcggtcag cgtcttcgcg
     3661 tccagcagac cgtccgtcgc gtccagaatc ccctgatcaa cagcgttcag cagcagcttg
     3721 atcccttcct gcaccttgct ggcgggggcg gtcttgacct tgccatccag ccccatcagc
     3781 ttcatcatgg cgcccagcac cacgggagcc tgcaccttgg actcgtcata gcagatcagg
     3841 acgctgccgg ttcggggctc cgccttcacc tgatgaatca cgcccgtgct ttccagctgc
     3901 tgaaccattt gcccggtcaa ggcgggctcc tgcgttacgg cgggcacata cagccgcatc
     3961 cgtcccggaa ggctggagcg gacttcggcc accccgcgga aagaggggag tttcatcaaa
     4021 ttgtgtttgg gaggcttggg caggagcacc gcagcgcccg cagccaacgc cgcccatccg
     4081 atggcattca catgaatccc tcctgaaatg tgaaacgttt tccgtaaaat tagtctatgc
     4141 taactttgcg ggctaaatca aggcaaaggg ttcctttgcc gtttcctatt ttatcacggc
     4201 gcgttttttt gtcaatattt ttgtttttgg tacacaggat tttggcaaag aaaaaagcgg
     4261 cttccgaaac ccgggaaccg ctggaaggac aggactcaac agtcggatgc tcacgcttct
     4321 ttttggagat ccagccggac gatctgcatt ttcatcatgc cggcgcccaa gcgggctaaa
     4381 aagnnnnnnn nnnagacgga gaaataggcg cctacgtcct gaatgttggc gcttttgatc
     4441 cacgttttca gcatcagccg aacggccttc tttcccgctg cgtcaaacac cagcctgccg
     4501 ccggggaacg cgtccgccat ggccgtgaac agcgcccgaa cctgctcgtt cagaaaataa
     4561 tagaacacgc cagcagcgaa gaaaaccgcg ccgccggcag cgtcaatgcg gtcaaaccac
     4621 gaaaggtcgt tcaggtcaca ggcgatgttt tcctcccgct ccccggcggg gagcagctgg
     4681 ttccgcacgg cgatcacgtc gggaaagtcc agattgtaga gcctgcagga gccgttgtca
     4741 caggcgcggc ccgtagcgtc cagaccgcag cccagattga ctaccgccgc gttggggtgg
     4801 gttttcagat agtcccgcac
//
LOCUS       DAWAEA010000062         4801 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2783_length_4801_cov_2.809524, whole genome shotgun sequence.
ACCESSION   DAWAEA010000062 DAWAEA010000000
VERSION     DAWAEA010000062.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2783_length_4801_cov_2.809524"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1017 a         1626 c         1414 g          744 t
ORIGIN      
        1 ccatggtctc ctccgcgtcc gcaggggcca ggaagttgta gtaggcgtac tgataggggt
       61 gattttgcag gatgcccacg ccttccgccg ccatgcacag ggccagaatg cccgcgatgg
      121 ccgcgccgtt gagccctttc gcccgccgca gcagggtctc cagcccccat gccgccagca
      181 cgataaaccc ggcgtacagg aagtagaaat gccgccagcc gttgtagatc accggctggc
      241 gcagcatcgc ataccccatg aacagcgccc acagcagcgt ggcgcacagc agcagtagct
      301 tctccggctc ccgcaggcac ttgcccggat ggcggaacca ttcccgtgcc gcagccagct
      361 ggcctacggc gcacaggggg aagaaataca ggggcagggt gtagaacatc atccggggca
      421 gatagcttcg gggcaggggg gcgaccgcct gatcgtaccg ctggccccgg aacagcacca
      481 cgcccgtcca ccggctgaac tgcgaggcat ttttcaagac gtactgaaaa tattccagcg
      541 ggccgtccca tgcggctggg gtcagcagca ggaagaaccc gccgaaggag accagcgccg
      601 tcagcgcggc cagcaccgca gagcggttcc actgctttcg ggccgtcaca gagaccagcg
      661 ccgccacgga cagcatcccc cagatcgcca gcccgacgat cttggtattg gccgccagcg
      721 cgcccgccag cgagaagagc agcgcccgca ggggcgttgg gcttttccac agccgcaggg
      781 ccagatagaa gcaccacagc accagcgtca gcagggccat gtccttgttg ttgtaatgcc
      841 cctccgcaaa cagacggggc gacagcagca tcatggctac cccggccagc gccgggccga
      901 agccaaggcc catttcccgc aggagcagat acagccccaa cagccccagc gtgaacaatc
      961 cccagctgta ggcgtgccac agccaatgaa gcagggcgga gtcggtttcg gcaagccgca
     1021 gcgccggggc cagcaagtaa taggcgctct ggccatggtc tttttccaca ctttcggaaa
     1081 tacgctgaac gcccagccgg tcgtagtagg ccaccgcctg cctgtctccg aagcgaacgg
     1141 catactcctt cagattttgc cgcagaatct cctgttcgga cggctcgtca aagtatacgc
     1201 cgtaatcggc ggcggtgcaa aggccccatg taaaaatcaa tgtaaaaatt aacgcaaggc
     1261 caaccaccga agcggatatt tttttctttc tcacggctga acgtcctcct ttggcgcgca
     1321 gacgcagcag atcatggctc cgtagctatc gaaccggcac agaatttcct ctgccgccgg
     1381 aaggacttcg cccgccagca ccgcatagct ggtgttcacg atccgatagg cagagccgct
     1441 ttcctcactg tccgggtaaa cggcttcgaa ccgggcgcgg cgctcctcgg gccagtgatc
     1501 cagattgctc aaaacgccgc tgcgggtacg attgtccgtc cacgtcaccc ggatgggccg
     1561 gtcggcaggc gcgttttcct ccagccagcg aagggccgcg tcaatgccca cgttccagta
     1621 gtccagctcg tgccgctgct ccagcccgtt tcggctgacc agcggctgat agtaagcgaa
     1681 ctggaaggga tgattcagcg caaggcccgc gccctgcagg gccagcaggg cgcaaagccc
     1741 gcccgcggcc agccgccggg gaaccgtccg ccgtgccaga tgcgttcgaa tgcgccccag
     1801 cgcccagccc gccatggcca tcatggggaa gaacagaaaa taggcgtgcc gccagccgtt
     1861 gtagacccgc acccgcatga ccatcatgcc cagcagaggc agcagccagc aggaaaggca
     1921 cagaaccgcc gcaaagctct cctgcccgca cagggccgag gctttctgac gggcacaccg
     1981 gcgcaggacg agtcctccgc ccagcaggat caaccccagt tggaccagag gcatggtcag
     2041 ggcgatcatc accggcaggt aatagaacgg cgggcgctcc cacgaaaggt cgatcacccg
     2101 gccacagaac agcaccgtgt tgttccagcg ggaaaagctg taggagttgc gcagcacgta
     2161 ggtcaggtat gccgccgggt cccgaagcag agccggggtc agcagaacat agcagccaag
     2221 agaccccagt accgccacga gtaccgcccg ccatgtgcgg cgggtgaagt gcttttccag
     2281 caacagcgac agaagggcca taccgccgca aagaaggctg accgccccgc cgatgatgcg
     2341 ggtattggtg cagaaacccg ccgccagcgc gaagcacagc gcccggcccg ccgtgggctt
     2401 ttcccgcagg cgcagggtct gccacagcac caccgccgcc agatccatca gagccgcgtc
     2461 cttgttgttg taatgcccct gcgcgaacag acgggggcag cacagggcca gcagcgccgc
     2521 cgccgcggcc agcgggcggg agaggcccat ccggcggcag acggcataca gggcgtacag
     2581 gcccagcgta aagagcgccc acgtccagac gcgccaccag tgggtgaact gctggtcggt
     2641 cacgttcggg tcgcaggcca gcggcgcaag gggatagtaa gccgagctgt tgtgatccat
     2701 ctcgatgctc tgagacagag gaatgacccc catggccttg agcgcctgat gaaagcccgt
     2761 ctcaaagggc aggagctcac cgtattccat cagggccatg cggtaaatgt tgatctcgcc
     2821 cttttcatcc cagttacggc cgtaatcgtc tactgtgcac agccccagca ggaagatcag
     2881 cgcaaacaga agcgcgatcg ccgccttggc aagccgctgc cgggtctcgg gccgcagtct
     2941 gcggccgatt gcttcactca ttcgccttgc tcctttgtcc atgcccgatc acggtcaatg
     3001 cgcatcagtg tgcgtccata ggcgcggatc gtcacggctt cctcctgctc ctccgacggc
     3061 tcccatccgc tgaacaaggc gtaggtggga ttggacagca catattccgc cgcgccctcc
     3121 gtcgggataa agcgccgccg cagccgtggc tccatcacat agagggcgtt ctccagcccg
     3181 gcccgggccc acaggtcggc gggcgctatg atgatctcgc cttccgcctg cccggccagc
     3241 gtttccagcg cgttcagaac gctcacgttc cagtagtccc gttccagatc gtcctgcccg
     3301 gtcagcagac ggacgacggg ctgctcatag gcgtactggt acgggtgctg ggtaacgata
     3361 cccacgcccg tcagagccat gcacaggctc aacagaacgg tcaaaaggcg gcttgccaga
     3421 cgcttgcctt ccatcagccg gtacagaccg tgcagcccgt aggccgccag caccagcatg
     3481 ggcccgtaga gaaagtagaa atgccgccag ccattgtaca ccgtggcccc ggccgccaca
     3541 gccgccccaa gcggcagcag ccataggagg gtgcagagca gcagtcccag cgcccggtcg
     3601 ctgcgcccaa tgcccttttt ccagcttttc agcgccccca gctgaccggc ggcgcacaga
     3661 aacagcaccc acagcggcgt ggtcgctgcg atcatgtagg gcaggtaaaa cggtggaagc
     3721 cggtcttttc ccgccacagc gccggggtca gcagggcgta aaacccgata aacccgaaaa
     3781 acgccgccag cccggccgcc caaaccctgc ccccgccctg acggcggagc agctgccgca
     3841 gcagcacaaa cagggcgcac agcccccaaa gggcgaagcc gatcaccttc atgttggcac
     3901 agaaggctcc cgccaacgca aacagcagcc ctcgcaggac gcccggacgc ttcatcagcc
     3961 gaagggccgt ccagaggacg cagagggtca gggccatcag cacggtatcc ttgttgttgt
     4021 agtgcccctc cgcaaaaaag cgggggctca gcaacaagaa acaggccgcc gccagactga
     4081 caccccggga cagctccagc tcccggcagc agccgtacag cgccagcgca cccagcgtga
     4141 ataagaccca gcaccaaccg tgccatagaa tcatccggct gtgctcggtc aggttctcgt
     4201 ccatcactac gcccgccagc ggatagaacg ccgccgcgcc gtgatcccgc tcgatgcttt
     4261 cgctgatggg caaaagttcc gacgtggtgc cgttgccctg ctcctgccag taggcaaagg
     4321 cgctttcgtc cgctcccgcg gcccggacat actcccatag attcatccgc aggatgtcca
     4381 tttccagccg ttcgtcccac gggcggccat attggggaac cgtcaccagc cccaaaacca
     4441 gcaccagccc gaaaaacagc ggtatcagcc agcggggaag cgtccttctg tgcataaaag
     4501 gctcctttcg tgcagcgaaa cgactgtttc agaatgctct gctctaaata gaaacagcgc
     4561 ctctgccact ccgctctgac ctctttgggt caagacggtt caaagcagag acgttgcgtc
     4621 gggttctcaa accattaggg ggaaaaggaa aaaagtcgta attccaattt gcaagctgtt
     4681 ccgcctgtat caactgaccg tgtttaaaag gcgctgcccc ttgcgcaagc cgtggcaccc
     4741 gtcgtgccac ggcggcctgt ttcagccaac ttgcagaaaa tcacatcaga ccggcgagcc
     4801 g
//
LOCUS       DAWAEA010000063         4756 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2814_length_4756_cov_1.841949, whole genome shotgun sequence.
ACCESSION   DAWAEA010000063 DAWAEA010000000
VERSION     DAWAEA010000063.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4756)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2814_length_4756_cov_1.841949"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2307..2346
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3265..3274
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3845..3854
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4373..4517
                     /estimated_length=145
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          881 a         1423 c         1342 g          905 t
ORIGIN      
        1 cgtcctcctt tgccctgtgc ttcttccgat tttcccacaa tttagcatat cccagccatg
       61 aaaacaagca cgtttcttat ccccaaagac gacgtgtgtg aacttttcca gcaaaaaagc
      121 acggagagat ttttctccgt gcattcgtcc ccctctaagg ggattattgt ttttcccggc
      181 gctgccccag cttttccacg cccagcagcg ccagcaccag aaataccggc gcatacagga
      241 acaaatatct cggccagacc tcgaacagca gctgataagc cgtcagcccc agcagggaca
      301 gggcgcagag ctgcacggcg tactccctcc gccacaaaag gacggcggca aggcagccca
      361 gcagcaccat tagccagagg cactgcatca cggcccggta agcgggattc catgcgccct
      421 cccgatagaa gattttccgc aggaacagcg acagacggtc gttgcgccgc accggctcct
      481 ccaccagata gctgttgaag gcgaaagtgc cgtctgcgta agccttatag gctttgacgg
      541 aaaagaagtg cagattttcg cccagggtac ggttctgtac ttcttccagc gcccgggcca
      601 gattggcgcg ggtgcgctcc ttcgccgtgg caaagctgcc ggaataggtc acgccctcgg
      661 cggaatgacc gccccagtac tggtcatcca gcccgatcat gagataatgg gcgctgccca
      721 gcgcttcctc cggggccgtg gagcccgtca gcagcttgac ggagcctttt tccagcgcca
      781 taccgggcag aacgcccacg ataacggcgg caaggacgca cagaccccgc ttccacaggg
      841 cggcgttttt cttgcggaaa aggaaacgga tcgcgcccag cagcagagcg gcgatcagca
      901 ggatataggc gctgggcttc accgccccgc ccaatgagca gcacagcgcc gccagaccat
      961 agcgcaccgg cgctttccag tccgtcaaca ggatcagcat tcccagcacg ggaaacagac
     1021 aggcgtaggt gtccgtatag ggcatgatga taaagggcga aaacagcagc cataccgccg
     1081 ccagcccaaa accgacccaa cgaacagagg gacgcaccct gagccgccgc agcaccgcca
     1141 tggccgcggc cacgctgaga ttggtcagta gcgccgcgcc gtaaacctcc aacacatagg
     1201 gctcggccaa gcccagcttc tttcccagcc gatacaccag cgacaggagc accgtcagcg
     1261 gcgcgttgtt ggggcactga cggaaataga cgagccattc ctcggacagg ggctgaccca
     1321 aggccagcgc ttccgcacga catccgaacg gaagaagccg gctgcggcag cttggccggc
     1381 cgaactctca gccgccagac ccacaggccg gtcagccctg ccgccgccag cagcagaacc
     1441 cagttttcca cgcagaagcc atgggcataa tacccctcca tggcccgctg gcccagcgtc
     1501 agaagcccca gcacccacag gagcatggcg gcgtacagcc agcagaaaag gggagaaagc
     1561 cagtcgcggc tttctccctt cgaggttttg gtcatgcttt cacctgcttg gcgaaggtgt
     1621 ccttcagccc caccacccgg ttgaacacgg gcatttcagg ggtggaatcc tgatccttgc
     1681 agaagtagcc cacccgcagg aactgatagg tgtcgcccac attggcctcg cccagcgcct
     1741 tggaaagata gccgtggcac acttccaggc tgttggggtt cagcttcttg aggaaatcgg
     1801 tcttgagagt ggcactggac accacattct cctcgccgtc ggcctccgcc ttggcttcgg
     1861 cctcgctctg agcagcctcg gcggctgtct cctcctcggt ctccttcatc atcaggggct
     1921 cgtacagacg agcttcgaag ggaatattct cgctggcgct gacccagtgc agcgtgccct
     1981 tcaccttgcg gttggcaccc tcggtgccgt tgcgggtgtc catatccacg gtgcacagga
     2041 cggcggtcac atgaccgttt tcgtcctttt cacagccgtc gcagcgcacg atgtacgcgc
     2101 ccttgagccg cacctcgttg ccggggaaca tgcggaagta cttcttcaca ggcacttcca
     2161 tgaagtcctc ctgctcgatg tagatctccc gggtcagcac caccttatgg gtgccccatt
     2221 cgggatgatc gggatggttt tccatttcca gttcttccgc ccggtcctcc ggccagttct
     2281 gcagaacgac cttcagggga tgcaccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnngccc agatcctcgc gcacgcagtg ctccagcagc gccagatcga cggtgctgtc
     2401 cgccttggcc agaccgatgc ggtcgatgaa gtcatgaatg gcggcggggg tgtacccccg
     2461 gcggcgcatg gcgcacagcg tgggcatccg gggatcgtcc cagccggaca catagccctt
     2521 ttccaccagc atccgcagat aacgtttgga catgatggtc tgggtcaggt tcaggcggct
     2581 gaactcgatc tgccggggat ggccgggcag catattctgg gcgtgctcca ccacccagtc
     2641 gtacaagggg cggtgatctt catattccag cgagcacagg ctgtgggtga tgccctccag
     2701 cgcgtcgccg atggggtggg cgaagtcgta catggggtag atgcaccact tcttgccggt
     2761 gcggtgatgt tccttataca ggatgcggta catggccggg tcgcgcatat tgatgttggg
     2821 gctggccatg tcgatcttca tccgaaggat catgctgccc tcgggatgct tgcccgcttt
     2881 catttcctca aacaggcgca gactttcctc cacgggacgg tcgcggtagg ggctgttctt
     2941 gccgggcttg gtcagggtgc cgcggtattc gcgcacctgc tcggggctca gctcgcacac
     3001 ataggccaga ccgcgtttga tccagtccac agcgatctcg tagcacttgt cataatattc
     3061 gctggcgaag aacaaaccgc ccttccagtc gaagcccagc cagtggatgt cctccatgat
     3121 gccgtccacg tactcggtct cttccttggc ggggttggta tcgtcaaagc gcagattggt
     3181 cacgccgccg aatctttccg cagtctggaa atccgcgaac atggccttgc agtggccgat
     3241 atgcaggttg acgttgggtt cgggnnnnnn nnnncatcct ttcctgtatt gacggctgat
     3301 agcggcggtt ggccgcagaa agcagtcgtt tcgcccatta tagaacgaaa acgggcgttt
     3361 ggcaaggggc gcgggccacg cgcagcccta tttccctctt gttctttggg tctggcagga
     3421 ctatactgtg ggggagtttt tggaacgtca agggagatgg aaattcatgt gggctgtgta
     3481 tgcggtgctt tcctcggtat ttgccgcgct gacctcgatc ttggcgaaag tcggcatcga
     3541 cggggtcaat tccaccctgg cgacggccat tcgcacactg gtggtggtgg tcatgtcctg
     3601 gggcatggtg ttcctgacca acgcgcaggg cggccttgcc gaggtcagcc ggaaaagctg
     3661 gctgttcctg atcctgtccg gcctggccac gggcgcgtcg tggctgtgct actaccgggc
     3721 actgcagctg ggacaggcca gcaaggtggt gcccatcgac aagctgagcg tggtcatcac
     3781 gctgattctg gcgtgggtgt tcctgcacga gcagttcaac atgaaaagcc tgatcggcgc
     3841 ggcgnnnnnn nnnnaagccg gtcacgctct ggcggcgaac tcggcaaagg cattatccac
     3901 cgccgtttca aaggcgggat agcgcatgga cttatggatg gctttcaggg tcttgggtcc
     3961 cagccccatg cccttgccca gacaggccca gacttctttc attttgcaca gcacaaaggc
     4021 ttcggtgccg atctgcgccc ggtagccgtc atagagcatc tggtgaaacc ggcggagagc
     4081 ttcgacgctc tccgtttttc cattgtacag attcacaagc cccggatgat acagaaagcc
     4141 ccggccgcac atgacgcttt ccgttccggg atagcgccgc tcgaactccc gcagcgcttc
     4201 agcggtgaaa agatcaccgt tatagcacac ggggagtttg ctttcccgaa gggtgtagtc
     4261 gtaccactcc cgatgcgggc ttccgccgta ttgctccgcc cggctgcggg gatgcaggat
     4321 cagccgccgg gccgggtatc ggttgaacag ctccagcagg gcgggccatt ccnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnngga cagcccagat tgatgttgac ctcctcgtag cccagcgctt
     4561 tcagcttgtc cgccgcccag agaaagtcct cgctgctgtt ggacaggatt tggggcacgg
     4621 ccttcatacc ggcgttattc tccggcagaa cgtccgcctg ctcccgatgg gtcagcgact
     4681 ggccccgggg gctccaaaag ggcagaaaat attcgtccgc cccatcgaaa caggcgtgct
     4741 gggcgctgcg gtacag
//
LOCUS       DAWAEA010000064         4734 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2830_length_4734_cov_2.136354, whole genome shotgun sequence.
ACCESSION   DAWAEA010000064 DAWAEA010000000
VERSION     DAWAEA010000064.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2830_length_4734_cov_2.136354"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    460..469
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3124..3246
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4045..4054
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          867 a         1439 c         1413 g          872 t
ORIGIN      
        1 caccgtcacc ggcacgaaca gaggctcgga ctcggtgacg atgaccacgc ccaccgcatc
       61 gttctgggtg tacagcgccc gggagggcac ctccatgcaa tccacatatt cgcccagctg
      121 agcccggcag gtgcgcatat acagcacatc ggacacatcg cccagcacag ccagtcgaac
      181 cagcagttca ccgccggaac gggtggagga cagcacctgc gcgtccacta ccgtgctgga
      241 aaactgttcc agcaccagct tgtaggtgtc cccgtcccgg gggctccacg tgtcgttttt
      301 caccagcatc aggccggcgt agttgccttc cttcaccagc cggtacaggt cggtgcggcc
      361 cctggcggcg gtgccacggt cgggcttcac gccgttgatc atggaacgca cctgagaggg
      421 cgtgtagctt tcataggcgc tgggggtcag cgcgtattcn nnnnnnnnng ctctgctgca
      481 tggcccgctt gggcttgatc cagctttcga tgcgcttttt ctgggtggct tcgtcgtcgt
      541 agagccggtt caggcgcata tcggtggaat atttggtgcg gaagtagtcc tgccgctggg
      601 tgatggccgt ggccagaatc tgctcctgat tggtcagatt gccccgtgcg ccgtgcacca
      661 gactgcgcac ctccagtcca cgctccagca cctcgttttc cagccgctcc atgcgctggt
      721 caaagtccgt ttcgctggcc agaaggcttt gctgatagtc gttgatggcg tcccggtcgt
      781 tctgcagggc ggtcatttcg gcggtactgt agccggtgga atacacgtaa caaagaatat
      841 cgccgctgta gaccacgctg ccctcctggg ctacgtattc gatggactga acgccctcgt
      901 cgtcaaaggc cgtctcgttt cggacgatca gcgcgtcacc ggtataggtg gtgccgatgg
      961 aactggcctt taccgtagcg gtggcggtcc gctgcccgga gccgaagcgg ctgtaggcat
     1021 aaacgccgcc gatgatggcc gcgcccagaa gaagcgccaa cacccgtttt ccgccgcccg
     1081 gtttccgggg ctttttcggc ttcttgagct tcttccccgg ctgattgtag gggttgggct
     1141 gaatgggagg catctgcggg ggcatattgg gcggcacaaa ggctccgccc tgcatgggct
     1201 gggaaagatc cggctgctgc atgatgggct gctggccgaa gtcctgcacc ggctgaccgt
     1261 atacgccgcc ttggggaaca aaggctcccg ggtgctgaaa gggcggcatt tgctggggct
     1321 gctgggaaaa atccccatac cccggcatga ccggcggcat ctgctgcgta taatcaaagg
     1381 gctgaccctg cggcacaaag gcgccgggct gacccgcgcc gtaaggggac gcgaaggttc
     1441 cgtctgcggg ctggccgttc tggggcggaa cggcgttggg cgaaaagccg gagggcgctc
     1501 cgccctgatt gtagaaatcc ggatcctgct gcatcgccgt tctccctcct tctgtgaatt
     1561 cgatcgtatt tcagtggggc gcgccgccgg tcagcgggcg ccctccatgg cgcgcagacg
     1621 ttccagcacc cggcgttcca tgcgggaaac ctgcatctgg gaaacgccca tccgtttggc
     1681 cgtctcccgc tggcccagcc gctcctcata gcgcaggagc aagagttgtt tttcctgcgg
     1741 ggtgacgcgg ctcaggatcc attccatcca ctggccgcgt tcctggcgtt ccagctccgg
     1801 gtcatcttcg cccaaaagat cggccagatc ggtctcgccg gtctcgtcca ccggcgcgtc
     1861 cagcgatacc acttccgtct gccgtttcag gttgatgagg gcgatcattt cctcgggagt
     1921 aatgcccatt tcctgcgcca gctccatggc gctgggggtg cgcaggtgct cctgctcgaa
     1981 gcgctcctgc agccgggcca tctggtacag ccgctgccgt gcgtcccggg gagcacgcac
     2041 catgctgccc ttatcccgca gataattgcg cagatcgccg gtgatggtgg gcaccgcgta
     2101 ggtaacgaag cgcaggttcc ggtctggctc gtagcgttcc agcgccttca gaagtcccat
     2161 ggccgccacc tgctccagat cctcccgggg cacgccccga ccggtgaact tggcggcaat
     2221 ggctttcgac agcgggagca gcccctcgaa cagacggtca cgcagctccc gatcccgggt
     2281 ctgggcatat tcgtgcagtc cctgtgacag ttgctcgttt ttcatcggag ccaccgccta
     2341 ctctgccaga gaaccgcaaa tgccgtacac gccgtgctcg tcggtctcca gacggacttc
     2401 cggcagcagc gtttccagca cgcagcgggc gatctccgcg tccagcgcat ccgccgggcc
     2461 ctgtcctgct tccccttcgg cggaaaagcg gaattgcagc cgtccttttt ccgcccgggc
     2521 gctgagtacc agcgtttcgg gggtggtggt ctgatgaagg aggcagtcgc agcattcgtt
     2581 gacggcagtc tgcaggtcgc cgatcagatc agcgtccagc cccgccagca tacccagccc
     2641 gctcagggcc atgcgggcga tcagccagtc ctcctgccgc gccgacagcc gaagaaccat
     2701 ttcgtggggc ctgtccatag ccagtttgcc tccttccggc ggaaataccc aaggatagcc
     2761 ataattgtaa cataaatgta aagaacaacg caagcaatta agaaaacttt gttacgcctt
     2821 tttcatgatt cttttcggga gttttggcag aaaagccggt ttttttcggg aaatggcagg
     2881 aaccgctggg cgcacaggca caggggcgtc agagccgcca caaaggagaa gaaccgtgcg
     2941 tcctgcccgc aaagcagcag catggtggcc agacagtagc acaggagggg aaagaccggc
     3001 aaaagccttt ccgtgccgcc gtggtacagc gcccacaggg tcgcgagcag caggagcagc
     3061 agctgcgcgc cggtgttccg gctcagccag cgcaggggaa gaaattccag cggcgcggac
     3121 agannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnntgga aagcaggctc gtcatcgtct gagactgaat cttttcccgg aagaatgtga
     3301 acttgatgga attatagtcc gaccggcggt aggtgttctg ccagccgttc tcgtcggcca
     3361 gcgtgtccag aaacgcccgg gtctcgccat ccagaagctc cggctcccgc tggcgggcgt
     3421 tgcacagcag ggtcatgggc aggcccacgg cctcttccag cgtgttgtcg ggatagacga
     3481 tgtccagccg gctgtacagc ccgccctgga tcagcacaag gcagccaagg cagcacagcg
     3541 cacccgtcag cacacctttt ttctgcctgc ggaaatagca cccggcggcc agcagcccca
     3601 gcggaagggt cagcagaaag ccgttctgcc gcaccagcgt ggtaaaggcc agcgccagcc
     3661 cgaagcagag agcgttgcgg gcgctggaaa gccacgcgcc ccgggtgcgg atcagctgaa
     3721 ccgtctgccc gaaaagtagc agacagccga tggtcatggc gttgtccttg ccgacgtaca
     3781 tcatcgtgtt gcgcacgggc tccatcagcg ccgtcagaaa ggcgcatgaa aggcacagcc
     3841 agcggggcgc gccggtgcgg cgcacagatg cggcggcata acccagcgcc agcgagaagc
     3901 acagaatctg caccagcacc gcgaagctgt aatggttgta cacccgcgtg accagccaga
     3961 tcagcagcgt atggaacacc gggtgccagt tgttgaactg gccgctttgg gcctgctgcc
     4021 attgattggc cgcgtcgtag ctgannnnnn nnnnagtcct tttgctgaac cgccgcgggc
     4081 cgcagccggg gacaggccca caggaacata agaaacagcc ctccgctgaa aagcaggaac
     4141 cacagaaccg ccgcggcggt ctgtacgccc ccggtcagga acgcgctctg gctgaaataa
     4201 agcacatagg ccccgtaaaa cgccaaacac accgcgcaca ggaaaaacca tgcgcggtgt
     4261 gccttgcttt gccaatgatc gtgccggaaa catgaatgca ccttcatcgc tcctttgtgg
     4321 aaaaagacga tcattccgaa acaaattcgt cgtaaatggc cagaatggcc tgctgaaatt
     4381 ctccgctgtc cacgcccacc agaatgctca gctcgctgct gccctggtcg atcatgcgga
     4441 cgttgatgtg atagcggctc aacgcggtga acagccgggc ggcggtgccg ggattgtgca
     4501 ccatgccccg gcctacggtg gcgatgatgc ccatgttatc gtgaatggtc agggtgtcgg
     4561 ggttggtggt ttcccagatg cgccgggtca gctgttcctt caccggctcc agttcggcag
     4621 tggaaagcac cacgcacatg gtgtcgatgc ctgtgggcat atgctcaaaa gacaggccga
     4681 actcctccac cgcctgcagc accttgcggc cgaagcctac ctcgccgttc atca
//
LOCUS       DAWAEA010000065         4697 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2857_length_4697_cov_2.544377, whole genome shotgun sequence.
ACCESSION   DAWAEA010000065 DAWAEA010000000
VERSION     DAWAEA010000065.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2857_length_4697_cov_2.544377"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1974..2137
                     /estimated_length=164
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          882 a         1384 c         1415 g          852 t
ORIGIN      
        1 gtactttctt tcggcgcgaa agaaagtacc aaagaaagca cgcggcacgg cgactacggg
       61 aaaaagccct ttactgccca ttttgacggc ggggctcgca atgtcgcgcg caatgaattt
      121 gggcagcttt cacttactct tggagcgcgc tcgtgctcgc ctttttccgc cgtcaaaatg
      181 ggcgggcctt tttccctccg ctgcctatcg ccgctcggct cccccacact tggcgtaggg
      241 catactcaaa tggcgatgct tgggatgatt cggcgtgggg cggctgattt tgcaaggtaa
      301 agggttggtg gtgtccctaa atcatgttgg cctttggtgc gagccggagg gcctgtcgcc
      361 cgacggcagt ctgcatacgc caacaaacag gaaaacacac caagcccgcg agccgcgagg
      421 cccgtcgccg agcggcggta actccgcagc caacttgctt tccctcgagc ggccccgcgc
      481 ccatagggcg cggtatgggg tgcagggggt caccccctgc cgcgagccgg agggcctgtt
      541 gctcgacggc ggtctgtacc agtcaacaaa caggaaacta caccagacag gcgagccgcg
      601 aggcccgtcg ccgagcggcg gtaaaacccc ggccaactag ctttccctcg agcgccctgc
      661 gcgcctatgg cgcgcagtcc gaatggggct cagcccctag ccctgcctgc ggggaaggag
      721 gaagaaggcg gcggccagga ggatcagaag ggggagggcc acgagaaggg cgacgcccat
      781 ggtctggctg cccacggtca gggaggggcg gaaggcgctg gaggccataa tgtccagcga
      841 gatttcgccg ctgggaacca gctccttgag aacagcggtc agaaactgct cgttgaaggt
      901 gctttggtaa atgtactcat cggtcagcat ggtgctgttg ccaagggcga acatccggct
      961 gacgttgccg ttggagtgca tccgatgggc gtatagggcc agagaaagct cgccttcccg
     1021 gtcgccctcc tgcttgtcca gcgtgttcct gccgtccgtc agactgcgca ggtagccgtg
     1081 gggtccggtc ttcagcaccg tggcgacggt caggctgctg tccggctcgg caggatcctc
     1141 aaaggcgctg gcggcaggca tcaggagcac gtcgtagccg ctgctgagca aagcggcggt
     1201 catgtccagc tcgcagacct tcggaatgat gtagagaggt tcaccgtagt aggtgtcggt
     1261 gtcctcctcc ccggccacgg ccacgcccgc cagaggcacg atgccgtagc tgcgcagaaa
     1321 ggccagatag ttgggcacgc cctcgatggg gtcggtgtag tcccgcacgg caaagacgtt
     1381 gccgccgttt tgggtgtagg caagcagggc ttgggcttcg tcctcggtca ggtctttttg
     1441 cagaccgacg atgaacagac agtcgagccc gtccagtacg tcccccgtgc gcaggttcac
     1501 gctgcggctg tcgatgttgt tgctggtgag gaagttggtg aagttttcca gattttccag
     1561 cgtcagctcg ccgtgaccct gcaaaaagcc cacggtggga atttcctccc gggtgacgta
     1621 tacgatggcc tccgtcaggg tcttttcgta gctgagaccc tcgatctgga actccccggc
     1681 ctcaatgttg tagccggtag tgaagaaatt ctgataatcc agcaccttgt agcgcccggt
     1741 ggccgggcag gagacgatga cggaatccgc cgcaacggag gtggtctcgt cgccggtgaa
     1801 ccgggacaga atggccgggt tccgggcgat gtctgtggga atgacgctga tgtggtcgga
     1861 cagcaccttg tagcggttca gcacctgcag gacctgctcg tcctcgtccc cgttctggta
     1921 gagaagatac agctccacgt cgtcctgcac ccgctccagc gccttttgcg tttnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcc atgaaacgct gacgttgctg
     2161 gccagaaggt cggtcatcca cagaaagaaa ttcgctccga agcccgccac cgcgccggtg
     2221 atctggtttt tggcaaagca gctcaccagc agatccagcg aaagaaagct caggctttgc
     2281 agcgtgaagc ccagataacc cacgaaccat tccccggggt agaccgtgcc gtaaaaggcg
     2341 atgatcaggg tgtacagatt gctgcacaga atggtcaaaa gcagcaccgc cgccgccgcg
     2401 aagtatttgc ccagcaccgc cgaggccagc gataccgggg cggtcagcag cagctggtcg
     2461 gtgcgcttct gccgctcctc gcagatcagc cgcatggtca aaagcggaca cagcagcatg
     2521 ttcaaaagcg tcagctggga caggaaggac agaaggtcgc ccgacaggct tttcaggttg
     2581 gaaagataaa agatcagccc ggccagcgcc agaaacacgc ccatgaacac atagcccacc
     2641 ggcgtgcggt aatagccgag aaattcccgt ttgaaaatgg ttctcacgtc cgatcttctc
     2701 ctttcactcg tccgcgatgg cctgcaggaa tacctgctcc agatcgccgt tctggcggct
     2761 cagatgaagc agcggcacgc ccagtcggct caaaagcgca aacagccgcc gctccggctc
     2821 gctttctccg gcagcgaaga ctaaggtcag cgtggttcgg tcaggatggc tgtccggctg
     2881 gacggtcagt cgccgcaggc cgtccaactc ccgcagtcgg ggcacgatgg atttttccgc
     2941 cgcgcctacc gtcgccagca gggaaaccgc gtccgtgctg tccgtttcgt ccagcgccat
     3001 ggcggtcttt acctgcccct gatgcagaat gaccacgtgg tcgcaaagct gctgcacctc
     3061 gctgagaata tgggaagaaa acagaatggt cttttcccgg cccagacggc ggatgagggc
     3121 gcgaatttcg gtgatctgct tgggatccag ccccacggtg ggctcgtcca gcaccagtat
     3181 ctccgggtcg ccgcacagcg cctgcgccag ccccgcccgc tggcggtagc ccttgctcag
     3241 gtgggacagc agccgccgct gcatctgagt caggccgcac agctccatga tctcatccac
     3301 atgagcgggc acggcccggc tttccacccc tttgagccgg gcggcgaagc gcaggtattc
     3361 ccgcacggtc atttcgtcgt acaggggcgg gacttcgggc agatatccca gatgcttccg
     3421 ggcctcctcc ggctccagca gcgcgtcgtg cccggccagc agggcccggc cctcggtggg
     3481 ggccagatag cccgtcagaa tattgatcag cgtggtcttg cccgcgccgt tctgccccag
     3541 cagccccagc accgagccct tttccacatg cagcgacaca tcccgcaggg cgtacagcgc
     3601 gccgtagcgt ttggtcacat gctgaatatc gatcacagaa aaacctccct tggatggcaa
     3661 aatggccgtt tctatcataa cacattcccg ccggtcacgt gtgactgaat tgtaaatctg
     3721 aaggggaggg aaatgcaaaa aggccgcagc tgcggcctgt gattgtcaaa aaagctcgcc
     3781 tgcggcgatc tttttcgcca gaagctgtcg gtgctgtttt cagcaaagct gaaaactccc
     3841 cgcccgaccg ggtgctgcgc acggcgaagc cttacggggc catttgccga ccgcaggtca
     3901 gccggtcgat acgaatacag tcagaggggc tgccgcccct ctgacaactc ccctgaagag
     3961 tttttcgaca gatactgttg cggagtgcgg cgcgaggcgg ggctgttcac aaaaagggtg
     4021 cgaagcgccg aggggcggga acagcggcga agccgctcct cagcccctgt tttcatccat
     4081 ttccctcagc tcagccggta cacccgggcc tcgtagggac ggagagcgaa cgaatcctcc
     4141 cgggagacag gcaggttggc gatcagcaat tcggcgtccg cccagcccat ggccgacagc
     4201 tccagcctgc cggcggcttc gccggaaaag ttgcacagca ccagcaggcg cgtatcgtcc
     4261 agacggcgtt cataggcgaa aatctgcgga tcctccggaa gcgccagccg gtaatcgcca
     4321 tacacgatga cgggattttc cctgcgcagg cggatcaggg cgcggtagta attcagcacg
     4381 ctgtttggat cgttctcctg cgcggcggcg tggatgaaag gatagttggg gttgacgcgg
     4441 atccaagggg tgccggtggt gaagccggcg ttgggcgtat cgtcccactg cataggggta
     4501 cgggcgttgt cgcggccctt ggcccggatg caggccagca tctcctcggg cttcacgtcg
     4561 ccgtcctcca ccagctccct ccatgcgttg agaatttcaa tatcccggta atcctcgatg
     4621 cggtcgaagg gaacgttggt catgcccagc tcctcgccct gatagatgta gggcgtaccc
     4681 tgcatcatat gcagcag
//
LOCUS       DAWAEA010000066         4680 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2863_length_4680_cov_3.435676, whole genome shotgun sequence.
ACCESSION   DAWAEA010000066 DAWAEA010000000
VERSION     DAWAEA010000066.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2863_length_4680_cov_3.435676"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1107..1350
                     /estimated_length=244
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2308..2317
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1004 a         1237 c         1270 g          915 t
ORIGIN      
        1 attgcccctt ctgcagaaag cacaccgtcc ataccgagac gaagtaaccg gaaggagata
       61 ttgacatggc tgaagagaag aaggtcagta aagatcgcgg caagtggttc cgcgaaatga
      121 gaagcgaact gaagaaggtc gtttggcctg atggcaagac taccgccaag aacaccggca
      181 ccgtcctgct gtgctccctg atcgtgggcg cggcgatctg gatctttgat gccgtggccg
      241 tgctggcggt ggataccctc atcggtctgt tctaaggtac aggacatggc tgataacgca
      301 aagtggtatg tagcacatac ctattccggc tatgaaaacg cggtaaaggc cgccatcgaa
      361 aagtccgtgg tgaaccgcgg gatggaggac atggtcctca agaccgagat cccgatggag
      421 acggtcaccg aggtcaccga gaccggcgtg atgaaggaag tgcagcggaa ggtatttccc
      481 ggctacgtcc tcatcaagat ggtcatgacg gacgatacct ggcacctgat ccgcaacatc
      541 cgcggcgtca ccggcttcgt gggcgaggcc aacaaggcta tccccctgac cgaggacgag
      601 gtggcggcgc tgggcatgga gaagcgcgag atcgtcgtgc tctaccatgt gggcgacacg
      661 gtgcgtatca ccgaaggccc gctgtccagc ttcacaggta ccgtggagga gatcgagccg
      721 gagaagaacc gggtgcgcgt ggtcgtatcc atgttcggac gcgagacccc cgttgatctc
      781 gaactggacc aggtcgaggt cagcagcaac tgagcgcaga gcgtctgcgc ggaagtccgg
      841 cgcaggtgtg ccggcaaagt gggagagacg ctgcgcgtct cgccaaaagc ggcaagtatc
      901 cccgccgcta ccacatttta ttgaaggagg tgcctacaaa tggcacagaa agtagttgga
      961 tacgtcaaac tgcagatccc cgcgggcaaa gcaactcccg caccccccgt tggcccggcc
     1021 ctgggtcagc acggcatcaa catcgctgcc tttaccaagg aattcaacga gcgcaccaag
     1081 aatgacatgg gcctgatcat ccccgtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gagttcaacg agcgcaccaa gaatgacatg
     1381 ggcctgatca tccccgtggt catcaccgtg tacgttgacc gttcctttag ctttatcacc
     1441 aagacccctc ccgcggctgt cctcatcaag aaggagtgca acattgagtc cggttccggc
     1501 gttcccaaca aggagaaggt cgccaccatt tccaaggcct ccattcagaa gatcgccgag
     1561 ctgaagatgc gcgacctgaa cgccgcgtct ctggaagccg ccatgagcat gatcgccggt
     1621 accgcccgtt ccatgggcgt cgtggtcggc gagtaaggag ggtgtaagaa atgtttagag
     1681 gtaagaaata tcaagagagc gccaagcaga tcgacaaggc tacgctgtat gatcccaagg
     1741 agggtctgga gctgatctgc aagaccgctt ccgccaagtt cgacgagacc gtggagctgc
     1801 acgtcaagct gggcgtggac tcccgtcacg ctgaccagca ggtccgcggc gccatcgtcc
     1861 tgcccaacgg caccggcaag tccgtccgcg tcctggtgtt cgccaagggc gacaaggccg
     1921 aggctgccaa ggccgccggc gccgactacg tgggcgagat ggacctggtg gagaagatcc
     1981 agaaggaaaa ctggttcgac ttcgacgtgg tcgtcgccac tcccgatatg atgggcgtcg
     2041 ttggccgtct gggtaaggtc ctgggcccca agggcctgat gccctctccc aagtccggca
     2101 ccgtgactcc cgacgccgcc aaggctgttc aggaggcgaa ggccggtaag gtcgagtatc
     2161 gtctggacaa gaccaacatc atccactgcc ccatcggcaa ggtctccttc ggcgtggaga
     2221 agcttcagga aaacttcgac accctgatgg acgccattgt gaaggccaag ccctctgcgg
     2281 ctaatggcca gtatgtccgc tcctgcgnnn nnnnnnncgc tgacaagctg tttgagaact
     2341 attccgccct catcggcgct atcatcaagg ccaagcccgc cgctgccaag ggtcagtata
     2401 tcaagtcctg tgtggccgct tccaccatgg gccccggcgt gaagatgaac gccaacaagc
     2461 agctgtaatt ttgtgagggc accgcgatac cgactccacc gcacagctca ccggcagatt
     2521 tcccgcaggc ctgcgttgaa gcctcttgaa atacacaaag tattcctgcg agacttcgcc
     2581 ttgcccagcg aaaaatctgc gcggagatct gaacggcggc tcggtatctt ggtacccggg
     2641 gcgctttcag gaagtgtcct gaaaatgccg gaaaattgtt atctttggct tgacaagctg
     2701 tgaagccttg gatataatat tggttgcgtt ccaaagacag caggtggcgc aagccgtaag
     2761 tcctgccgag gatggcaagt cattttgatt gcttttaccg tcctcatgtc ctttggcatg
     2821 aggacggttt ccttttttga acgtaccgac aattctaacg gaggtgaaat caaagaatgc
     2881 ccaacgcaaa agttctgtct gaaaagcagg cgatcgtggc ggcgctgacc gaaaagcaga
     2941 atgcagccgc cggtgtcatc gttgattaca agggcatcac cgtggcagaa gataccgagc
     3001 tgcgcgcgga gtgccgtaag aacaacattg agtacgctgt catcaagaat accctgctgc
     3061 gtttcgcttt caacaactgc ggtctgaacg aactggacga tcagctcaac ggtaccactt
     3121 ctctggctat ctgtgttgac gatcccgtcg ccccggcccg tgtgatggcg gactacgcaa
     3181 agaagctgaa ggacaagttc gagatcaagg gcggttttat ggaaggcaag gtcgtctcca
     3241 tggagaccgt gaacgcgctg gcgtccattc ccgccctgcc cgtgctgcag gcgcaggtcc
     3301 tgggcaccat gctggcccct atttctggtc tggcttgcgt gctgaagcag attgccgaga
     3361 agaacggcgc tgccgccgag taattcggct ccctttccat cgcaggcaca gggcctgcac
     3421 aacatcaatt taaatcttac aaataggagg ctatcacaat gtctgagaag attactgcta
     3481 tgatcgaaga gatcaagggt ttgaccgttc tggagctgtc cgagctggtg cacgctctgg
     3541 aggaggagtt cggcgtttcc gccgccgcta tggctgctcc cgccgccggc gctgctgctc
     3601 ccgccgctga ggagaagacc gagttcgacg tggtcctggc tggcttcgac gccgctcaga
     3661 agatcaaggt catcaaggtc gtccgcgaaa tcaccggtct gggcctggcc gaggccaagg
     3721 ctgccgtcga gggcgtgccc cacaccctga aggaaggcct gtccaaggac gatgccgagg
     3781 ctatgaagaa gcaggtcgaa gaggccggcg gcaaggtcga gctgaagtaa gttcttttgc
     3841 aagcaaaaaa cagccaccca tccgggtggc tgttttttcg cggccggaac agccagcttt
     3901 tcccgcttta caaagccccg cggcgaattg ccgggtggtc gtaaaacagt aaaatcaaaa
     3961 aatggtgaaa accttgtgtt ttcaaggggc ggcgtgactt cggtcacgcc gtttcctttt
     4021 tcatcagttc attcagaggg ataaagccca gcccgtcgta cttgatgtgg atgtgctgta
     4081 cccgtttgcc gctggacttg tccggtgcgt ccacatagat ggcagacacc agttcgcgca
     4141 gcgcatagcc gtccaattct tcaataccca catacttgtg cgctttctga atgaacttct
     4201 caatgctttc gttctgtcgt tcctgtactt caatctcctg ctgcaaggtg atcgcttccg
     4261 cttccaactg cttctgctcc gcttcatagg tctggctcag catatcgtag cgttcatccg
     4321 tcagccgccc gctggcgttg tcctcataaa tcttgatgaa tcggcagtaa cgccgcagat
     4381 gcttccgccc ggcgagagac gggcgaatga tacgcgaaga ccgagataat tctttgcaaa
     4441 ctgatcaatg ggcgttggca aagctgatcg gtcgggcttg tccgcctcat ccccgaaaaa
     4501 gaaccggaga tgtaactttt tcgtgtacaa ctactcatgc ccccttgaca ggatgagcaa
     4561 ttcaggatat actatgagta gttggattgc tcagccatta ttatacgcac ttcaagtgct
     4621 cttaccaata gacttgcgca atttgatgtc gcaaactttt tgtgaacagg agtgattacc
//
LOCUS       DAWAEA010000067         4671 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2872_length_4671_cov_2.063258, whole genome shotgun sequence.
ACCESSION   DAWAEA010000067 DAWAEA010000000
VERSION     DAWAEA010000067.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2872_length_4671_cov_2.063258"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    870..879
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1897..1906
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1036 a         1373 c         1265 g          977 t
ORIGIN      
        1 cgccaatgtg gattcggaga cagccgaaaa gattgaagac attcttgccg aattatccgg
       61 tggcattgtt tttgttatca cgcatcattt ttcggatgat aagaaaaagg cttttgcaaa
      121 agagttccgc tttagtcata accacgtgga acacggagga atatgatgcg ggaaatcgta
      181 agggcaagac atgttctgct tgaattcaac ggacgtgaca aaaaaaagca agcagtattg
      241 acaaataact gcctaatact gcctgctttt tcgtttatta ttgctatgca cagcgcgtta
      301 cacaataccg taaaacgcat actcctagca catttttatc ctgatcctca aaagaaaacc
      361 tctacaagag tgcgctgtaa ctcaaatagc gcaacgtatc cctgtttttt cacaatgaca
      421 aagccaccga tgaatcagca tttctgcttt ccaatcggtg gttttgtttt gccgttcggg
      481 aaacgtctta aaagctcaat ttcttgaaat ccctaagttg acgtttcttt ggagcgtctc
      541 tttttcaaaa acagtctcgc ttacagcacg ttggaagaag tgcactccac cagcacgctg
      601 ccgtccttac ggcggctcag cttggccttg ttgccgctgc cgtcgtccag ggtcatcttt
      661 tccatttcgt tcagggtcat gaagttcacc gcgtcgcttt ccacaaaggc cacccagttt
      721 ttgcggtcgc ggacgggctt ctcggtatca gcggtcattt cttcgatgga tttgcgttcg
      781 ttgctcatcg gaaagttcct ccttgaaaaa tcgttcctga aaatgatacc gcagtctttt
      841 caaaaatgca agcggacggc cctcaggtgn nnnnnnnnnc ggtagcggct gtccagcgag
      901 cgggcgtagg cgttcaggct gcggatcagc tcctgacggg cggtatagtc cgccggcacc
      961 accacatcga agatcagatt gatgcgatcc tgccccatca ccatgcggaa gtcgtgcata
     1021 ctcagtgccg ggtcgatctc ccggagaaaa tcgttcatct gctggcgcac ccggttggtg
     1081 gtctcgtcct cggtggcgat gggatccatg tggatgcaga tgggcatgtg cagctcccgg
     1141 ccgatctccc gttccgcatt gtcgatgatc tcgtgaatgg ctacgatatt gcactggtcg
     1201 ctgacctccg catggacgga ggcgaagcag cggcccgggc cgtagtcgtg gatgaccaga
     1261 tcgtgaatgc ccagaatgcc ctcgtaattc agaagcagtt cccgcagacg gcggcttttt
     1321 tccgggtcgg gcttgccgcc cagcagccgg tccaccgtgt cccggcagac ctcatagccc
     1381 gccttgagca cgatcagcgc caccaccaga ccgatatagc catcgatgcg ccagccgaac
     1441 agggcgcaca gcccggtgga gatcagcacc gcgccggtag ccagcacgtc gctcaggctg
     1501 tccttggagg cggccagcag ggtgccgttg tcggtagccc ggcccagacg gcgatagaag
     1561 aaatacagcc atgccttcac cgcaatggaa acgaccagca ccgtcagtac cggccacgat
     1621 acgctcagcg ccgcgggcgt gaggatggcc tccatgccct ccttcaagag ggtaaaggcc
     1681 atcacgagga tcagcagtcc cacgcccagc gagcccagat actccatccg gccgtggccg
     1741 aagggatgct ccgcatccac gggcttacgg gccacgcggg tggagaccag cgccacaatg
     1801 ctgcccgccg cgtcggacag gttgttggcc gcgtccgcca gaatggccac cgagccgctg
     1861 agaagcccga tcagaaattt gccggcagac agaagcnnnn nnnnnntgcc cacgcaggag
     1921 cccagcgtgc cgtaggcggc gcggacttct ccgttttccg tattttcata atccttcaca
     1981 aaacggcgga tcagccatgc cgtcattttt cattcctcca ctgcgcgccg aaagacgcgg
     2041 atctcttttc attataacat tcttttcccg aacagtatat gctttggccg aaaaaagttc
     2101 gaatgccgaa aattgttttt tcttcttacg atgacttttt cgtatatccg ttatgcagat
     2161 tttcattctg aaaaatgcaa atttcagttt aaaaataaaa agagccgttt ccgcacttta
     2221 cttattttgc gtcatatgct atactatgtc ctgctgcggc aacggctgcc gctcctcctg
     2281 ccccaatggt gcattttgca ggatggaatc aaaaaacttt catttgttca ggccgagcct
     2341 gataagggag gttccgttat gccccgcacg atttccgaaa aagcccggcg gatcgctcca
     2401 tccgctaccc ttgccatgga tgcgaaggcc aaggccctga aagcacaggg cgtcagcgtc
     2461 gtcagctttg ccgccggaga acccgatttc gatacgcccc tgcctatccg tgacgccatg
     2521 aaggaagcta tggatcagaa tatgacccgc tatacgcccg taggcggcac ggccgaactg
     2581 aaggaggcca tcatccatcg ttttgaagag gatcacggcc tgcactatga cccgcaggag
     2641 atcattgtct ccaacggcgc gaagcattcg ctctataccc tgttccagac cattctcgac
     2701 cccggcgacg aggtcatcat ccccacgccc tgctgggtca gctacccgga aatggtgcgc
     2761 atggcaggag gaacgcccgt ttttgtcgaa atgaaaaagg agcacggttt cgtgcccacc
     2821 aacggggaaa ttctggccgc catcacgcct cgtaccaagg cgttcctgct gacatccccc
     2881 aacaacccca ccggctgcgt ctggaatcgg aagcagatgg aaaacgtgat gcagttggcc
     2941 gtcgagcacg atttcttcat cgtctccgac gaaatttacg agaagctgct ctacaccggc
     3001 gaaaagccct gctctatcgc ctccctcagc gaggaggcca aggctcatac actggtggtg
     3061 aacggcgtca gcaagtccta cgccatgacc gggttccgca tcggctatac cgccggcccc
     3121 cgggacgtta tcaaggccat gaccaactac cagagtcagg ccacctccgc gcccaacagc
     3181 gccgcccagc acgccgctgc cgtggcgctc agcggcgatc aggcctgtgt ggagaccatg
     3241 cgtcaggcct tccagcagcg ccgggaccgc atcgtggagc ggatcaacgc cattcccggc
     3301 ctgagctgca ataagccaga cggcgcgttt tatgtgatga tggacgtgcg gcccctgctg
     3361 ggcaaaaaga tcgacggtca ggcgatcgcc accagcagcg attttgccaa cgccctgctg
     3421 gaaaagcagc acgccgtggt ggtgcccggc gagtccttca tggcggacgg tttctgccgt
     3481 ctgagctacg ccaccagcat ggaggccatc gaggagggta tgaagcggct ggccaatttt
     3541 gtaaaggagc tgaactaaat gctcgaattc agcaaaaaaa ccaaccagtt gatgcagtcc
     3601 aagtataagt atcagctgca ggaagtggag gaacccaacc tctaccggga aattttcgat
     3661 tacgaggcca tcccgaagat cgccttcaac aaccgtctgg tgcctatctg cgtaccggat
     3721 gagatctgga tgacggatac caccttccgg gacggccagc agagcgtcag tccctttacg
     3781 ccccagcaga tcgtgaagct gttccagctg gaatcccggc tgggcggccc caagggtctg
     3841 atccggcaga gcgagttttt cctctatacc cccaaggatc gggaagcact ggagatgtgt
     3901 caggatctgg ggttggaatt cccggagatc accacgtgga tccgggccaa cgaaaaggac
     3961 ttcgagctgg tgaaggcagc gggcgtgaag gagaccggcg tactggtttc ctgcagcgac
     4021 taccacatct tcaaaaagat gcacctgaac cgctctcagg cgctggaaaa atatctggcc
     4081 atcgtcaagg cggcgctgga tcgcggcatt gtgccccgct gccacttcga ggacattacc
     4141 cgggcggact tctacggctt cgtgctgccc tttgcggagc agctgatgga tctgagcgcc
     4201 gaaaccggta ttcccatcaa gatccgtgcc tgcgacacca tgggctacgg cgtcagctat
     4261 cccggcgcgg ccctgccccg cagcgtgccc ggcatcgtct acggtctgca ccattacgcg
     4321 caggttccgt cctctcagct ggaatggcac gggcataacg acttctacaa ggtggtcagc
     4381 aacgcgggca cggcgtggct gtacggctgc tccagcgtga actgctcgct gctgggcatt
     4441 ggtgaacgta caggcaactg cccgttggaa gccatggcca tcgaatacga ggccctgcgc
     4501 ggcaccaccg acggcatgga tctgaccgcc gtgaccgaga tcgccgagtt catggagcgg
     4561 gaaatcggat tggagatcag cccccgtcag cccttcgtgg gccgtcactt caacgtgacc
     4621 cgagccggca tccacgccga cggcctgctg aaagacgagg aaatctacaa c
//
LOCUS       DAWAEA010000068         4651 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2891_length_4651_cov_1.967363, whole genome shotgun sequence.
ACCESSION   DAWAEA010000068 DAWAEA010000000
VERSION     DAWAEA010000068.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2891_length_4651_cov_1.967363"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    234..243
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          923 a         1382 c         1435 g          901 t
ORIGIN      
        1 tttcggccag aatatcctga atatccgcct gataagggcg gtgggtcact ttccaggttc
       61 cgttcaggga aagagtcatg gaaaagctcc ctttttattc ttcttcgggc ggctgctcgt
      121 ccgcatcgtc tccgccctgc agataggaca gcaggttctc ctgattgacc agttccatca
      181 gctcggcggt gctttttacg cccatcttct gcagaatgtt ggggatgtgg gatnnnnnnn
      241 nnnccggaac tcctgccgca tccgctgggc gatctcctga cggatgggcg aagttccgcg
      301 gtaagcatcc agaatcgcct catggatctt ctccgtggag gcgtttttga acaggtagtc
      361 caccgcgccc atttcgtagg cgcggaaaat agtcgcatca tcgtcgtaaa cggtcagcat
      421 gatgatcttg gtctccggca gaatgctgag cacctgttgg cctgccttca ggcccgcgtc
      481 ccggctttcc atttccatgt ccagaatcag cacgtcggga tggtagagca cggcggaggc
      541 cacggcctga tagccgtttt ccgccattgg cagcagctcg atgtcctcca tggagcccag
      601 cgaataggca taccatttcc ggatggactg gtcgtcctcc gcgatcagta cccgaatctt
      661 catgcgaccg cctcctttgg ggtcaattct ttctcgtcag cgctgaattt cttcccagcg
      721 cacggccgtc agtgcgccgc cttcgccgat gccgcacaga tgcagacaga cggagtcgtc
      781 cgaccagccc ttgggcaggt agacccggcg actgcttccg ccccgaacct cggggtagcc
      841 ttccgttttc agttccaccc ggccgcacac gtgaccgtgg gccaccagcg tggcttcgat
      901 accgctgccc tccagcacca gtgtgcgggc gcggttctca ccctcaggca gaatgccgga
      961 cagcagcact tcctgtcccg gagacagcga tacgggcagc cgcactgcct tgccgccctc
     1021 ggcgggacga atatttctcc agacgttcac atccgcgccg ctcatggagc cttctgcgat
     1081 ccggcggccg ctgagcaggt gcgcccgtcc cagcgtggtg tggctgaacc ggcgaaccgt
     1141 atgcagccgc agcgttgcgc cgccgcgcag gccctccggc aggttcagcc gcagcaggga
     1201 aacgcccggc tgcagcacgc ccacgggctg atcgttgacg aagaccgtga cctgagaatc
     1261 cgggtattcc agctgcagcc agaagcagtc gcagtcgggc cggaaataga ccctgcgctc
     1321 aaaatccgcg tccatggggg cggagggata gctccagctg ttcagagtcg tggccagaat
     1381 gcggtcagtc tcccgcaggt cgggccggtt ctgacggcgg tacttgggat aggggaagat
     1441 ataccacaga tcggtgatct cctgctgctc cacaatgcag gccgcgccct cgatgccctt
     1501 gccgctgccc atatgcaggg agggctggcg cacgtcgtcg aagttcacgt ggccccagga
     1561 ctgggcgcgc acctgccatt cgccttccgc tgcgggcagc agaaggtcgg aaccatcgca
     1621 gtacagcgct ttcgttccgt ttccgttgct gatccacagg aaatcggcgg ggctgagcag
     1681 catgacgggg gcgttgccct tcacggagaa acggtagaat acgtcgccgc ggtactgacc
     1741 ggcgttctcc atggagcaga gccggtctgc cagcgggtga gccagcccct tcacggaaac
     1801 gtcctgcgcc cggaaatggt tggtctcccg ggcagatacc acggagggga tcgcctcggt
     1861 cagcgggggc agggagggac actcgaaaac ggcttccgag cggggcagaa tgcgaacccg
     1921 cacgccgttc agttccgtcc attcccggcc ggtcacgatg tgcttttcgc cgcccacaga
     1981 gagcatggcc cgggcgtttt cgccgcccac aaagctgact tccagcccgt tttccgtttg
     2041 ccggaaggac agaatttccg cttccgccca caggatggaa gcgcccttgc agccatagcc
     2101 ggacagatcc agcttccacg gcaaaatgac ggtcttgtgc ccgtgcactg tcaggctggc
     2161 ctcgccgtct tcggtgtcaa aacggaggat tccgtctttt tcgcccagat tggacgcgcc
     2221 cataagccat ccgcagacca gctccaccga cgggcacagg tcagcgtccc gtccgtcctc
     2281 gcccagcagc tgggcgcagg ggaagtcgca gcgcagcgtc gtaggcgcgg cacagggctt
     2341 ggaggcggaa agcttttcgc ccagcgcatt catcatggaa gccatcaacc gggcctcggc
     2401 ggcttcctcc cgcagacggc cgtccacggt gatcatggaa acaaagtcat agtcggaggc
     2461 catcagcgcc aggggcttgg caatatcgga ggcccagttg ctgatgccgt tggtcatgtc
     2521 gaaatccgtg ccgcctacct gattgtaggg gctgatgagc cgggcaccgc acagcagctc
     2581 ccgcttgagg aaggcgtgga cgcggttggt ctcggtgatc atcagcgggc cgccgcgctg
     2641 ggccgccagc tcccgcatgt ggtggagctg ggtttccaga tgggcgaaat agtcgtcgga
     2701 ataggcgttg aaggcggggt aaatatcctc cacatagccg ctgcagccct gcacgtcgca
     2761 ttcgcccgca caggcgatca ggggcacatc gatgcctaat cctcgggcga tctcatgcag
     2821 ccgggtcata tagccggcgg gatcggtgca gccgtagaag tccagctcgt tttccagctg
     2881 tacggccacc acgctgcccc cgtgggtgaa ctggcgctca gcaacgatgg gcagcacctt
     2941 ttccagccag ccgctcagtg ccttcagata ggtctcgtca ttctgacgca gcaccgcgcc
     3001 cggctggtac agccagctgg gcagcgcacc gccgtcccac tcggagcaga tgtagggacc
     3061 gggccgggcg ataacataca gcccgtgccg cgccgccagc gccagaaagg cggacacgtc
     3121 atgctcgtct tcgaagtgcc attcgccggg gcgcagctca tggaagttcc agggaatata
     3181 gacgtcgatg cagttgtagc ccgccatgcg gagctgatcc atgcgctcct cccagttctc
     3241 ccggggaatg cggaaataaa acagcgacgc gcacagcagg gtcgtcgcct tgccgttcag
     3301 ttgaagctcg ttttcccgta gggtgaccgt gctcattgga attatccccc tttttgcagc
     3361 tgttttcatt ataggagccg ttttgaaaat gtcaattttt catgccggag aacgcgatgc
     3421 cttcggtgat ctgcttctca cagcagaaat agaggatcag aatgggcaat agcgcgcaca
     3481 ccgtaccggc catgaccacg ctgtattcca tggaatactg gctgcggaac aggctcagac
     3541 ccagctgcac cgtccgcttt tccggcgtct gcaggtagat cagcggagcc agatagtcgt
     3601 tccatacctt gatgaaggtg aagatcacca gcgcggaaat ggcggacttg gacaggggca
     3661 gcatgatccg ggtgaagata cggacgtgac cgcatccgtc gatgatggcc gcttcgctca
     3721 attccttggg caggctcatg aagaactgcc gcagcaggaa aatgccgaag gagctgaaaa
     3781 gctgcaggag gatcagcgcc agatgggtgt cgtacaagtg catcttcgag atcaggatga
     3841 actgcgggat catgaccgcc gtccacggga tcatcatggt gctgatgaac aggccgaaga
     3901 agaagttctt caggggcact tccatgcggg cgaaggcgta ggccgccgtt gccgacacca
     3961 gaaactgcag tgccgtggcc accacggtga tcttgatggt gttaaggtag tattgcagga
     4021 agttgatctc cgtccacacc cggacgtagt tgttccagtt gggcgggatg ggaaactgga
     4081 tcagggtgat gaccatgata atggcgaaca gcgtgtaggc gatggccgaa gcatagccca
     4141 tcttcacgtt gacgaagccc tcggtgtaga tgcggaagac cagcacgttg gtcgcccggc
     4201 cggggccgcc cttggtcagg atgttgatga ggtcgaactc ctggaacgcc gcgatgatga
     4261 tcgtgaccag acacaggaaa atggtggggg agagcatggg gacggtaatg ttggtgaagc
     4321 actgacgctt ggtagcgccg tcgatctccg ccgattcata cagatatttt gggatcccct
     4381 gcagaccggc cagcaggatg atcatgtaat agcgcccagt tttgatccag gagccatccg
     4441 ggcgggttgg aaatgcccac catgcgcaaa agctggttca gcgggccgct gggggcaagg
     4501 atcaggttcc atacggtagc aacggaaacc atggaggtca ggtaaggaaa aaagcagatg
     4561 gtacgcagcg ccgttttgcc gcgaattttg gaattgagca gcatcgccac cagcagggcg
     4621 acgatcagca cgccgggcgt aaataaaagg g
//
LOCUS       DAWAEA010000069         4610 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2920_length_4610_cov_2.353019, whole genome shotgun sequence.
ACCESSION   DAWAEA010000069 DAWAEA010000000
VERSION     DAWAEA010000069.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2920_length_4610_cov_2.353019"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1123..1132
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1020 a         1391 c         1207 g          982 t
ORIGIN      
        1 cgtttttacc agctcctgcc gctgggaaag cagctccagc agggccttgg tcatggtctc
       61 gggggtcatg tcctcctgca tcagcacatg agccagcccc tgccgctggt agcttttggc
      121 gttctcgatc tggtcgcccc ggctggctcc cagcggatag ggcaccagca gcataggctt
      181 gtgcagggcc agcagctcgc tcagggtatt gctgcccgcc cggctcagca caatgtccgc
      241 cacagccagc gcatccggca tttcaacgga caggaactcc ttctggcagt agccgggcag
      301 atctttcaga ctctcatcca gattaccctt gccgcacagg tgcagcacgt ccatgtgggg
      361 cagcagctcc ggcagggcct tgcgcaggca ggcgttcacg ctctgggcgc ccaggctgcc
      421 gccgggcatc aacagcacgg gtttgctgcc gtcaaagccc gccagcgcca gtcccgccgc
      481 ccggctgccc tgaaacagct ccggccgcag gggcgttccg gtcatcacgc ccttttcccc
      541 cagtgccttg gcgcattcgg ggaaggtagc cgccacccgc catgcgaact tggcgcagat
      601 gcggttggcc agtccggggg tcaggtcgct ttcgtggcag accgtgggca catggcacat
      661 ccacgcgccc atcaccaccg gcacgctgac gaagcctccc ttggaaaaca ccacgtcggg
      721 cctgagcttc cgcatcaggg agacgctttc tgccgccccg gcaatgacct tgaaggggtc
      781 ggaaaaattt tgcagactga aatagcggcg cagtttgccg cttttcaccg agtgataggt
      841 aatgcccgga atggcggcga tcatctgatg ctcgatgccg ttctccgtgc cgatgtaatg
      901 gatttcatac cctgctttct tcagccgggg gatcaggctc aggtgagggg tcacgtgccc
      961 cgctgtgccg ccgccggtca gtacgatccg ctttaccatg gaacaacctc cgtttccgtc
     1021 atccgtattt cagtatacca cacaaatgca agggatgcga ccccggcggc ggaaggttca
     1081 gccgattcgc ttcaattcgg tgttcagacg gctcaggata cgnnnnnnnn nngtccgaag
     1141 cggagcatga tgatgcgctt ttcccgggga ttgagccgct ccagcgcgtc ccggaggata
     1201 tcccgctcga tctccctgtc cagattgacg gacacggtgt cgggccgggt tcccagtacg
     1261 tcgctcagca gcagctcgtt gccgtcccag tcggtcttca gcggttcgtc cagactgacc
     1321 tccagccgca gccgggagga cttgcgcagg aacatcaata tttcgttttc gatgcaccgg
     1381 ctggcatagg tggccatctt gatcttttta tcgcagtcga aggtgcccac ggccttaatg
     1441 aggccgatgg tgccgatgga gatcagatcc tccaatggaa tgcccgtatt ctcaaaccgg
     1501 cgggaaataa agaccaccag ccggagatta tgctcgatca gggtggagcg ggccgcttcg
     1561 tcgtgatttt tcagccgccg ggtcatctcc cgctcctcct ccgggctcag gggcggcggc
     1621 agagggtccg gccctccgat atagtaaagc tcctgcggaa acagttttgc caatagccgc
     1681 tgaaaccgaa gctgcaaagt caagctgcgc tgatccatcg gtttttcctc cttgtatgcg
     1741 ccgggcggcg ctattcttcc agactgaccg gcactttttc gggcagcgcg gccaacggca
     1801 ccagcgcctg aataccgccg tactcctgcc cggtgaccgc caccaccacg tccgcgtcgc
     1861 agctgtgccc gttcaggtac aggcggcagc tgtcgggccg gaacacgggc agcaggctgc
     1921 tccccgccgc ggtacgcacg ctgatgagcc tcatccgtgg cggaagctcc tgatgctgca
     1981 cccagcgggc gatctccggc agcagcaccc gcacggctcg aaaaggcacc accaacaccg
     2041 gcaggccggt gatcgggtcc cgcagaagat tgcccgtatc caccatggcg ggcagcagta
     2101 cgctgtgctc gcccacggtc agctccacct gccgcaccgc cgtcatggac gagggggaca
     2161 gcgccgccag ccagtacagc agcaagcaga gggccgccgt agctgcccac gccgctccgg
     2221 gcggggccga ccgcagcagg acgctcagcg ttccgccgca gagcagggcc gcgcacagcg
     2281 tggtcaccag acagcgcagg caggcctgcc agccgaaatg tccaaagcag agaacgaccc
     2341 ctgcgggcag aagcaaaagc cacaggggag aagggagaaa aagggcgcat aatgcgccgg
     2401 caccgcccag cgccgccgcc agcatccatc gggacgggcg gggaagaggc agtcccgcca
     2461 gcctgccgcc cagcgggtag gcgcaggcgc aaagtcccat gttgcaaaga agataggttt
     2521 ccccatggat catggccttt cgctccctcc gcttcctgtt caagcccagt atacggcccc
     2581 cggcggcaaa agagcgtcga agcaaagcgc acggattgtc gaaacggcgg gcccacgcct
     2641 tcccagccta tgagcgcaaa gggaaaaaca gaacgaaaaa agctccgaag caaaactgct
     2701 tcgaagcgga agggggctca tatgaaagaa atgcccgtct ccgtggggag gcgggcattg
     2761 tttcaggcag gcatttcgtc gtaagcgtag ctgtcctgat acatcgtttc cggggcaatg
     2821 tcgatttcgc cgtccagcca gaccacaacg ccgtgttcca ccttggcgtt tttgaacacg
     2881 ctgtcgtcct tcaagggttg aaacgcggga taatccagca acggcgttgc gtcatacagg
     2941 cgtttttcgc cggaagcaaa ggtcaggatc atcattttat catccagcgg ctttacggaa
     3001 agaacccgta tttccttcat gggtgccgcc gcataggcaa taccatcttt gataaacata
     3061 gactgcctcc ttatgccaac ggagcaattt tttcaaaagg ttcgccgcga acagctttgt
     3121 tccacgcggc atagacttct tcttcgtgga gggccagcca gcccaccacg attctgagct
     3181 gtttcacagg cagagctccc gccagcaatt caccatcaac gccaacagac gcttcatatt
     3241 ccccatagaa cacatgaaca tggggcttgt tgtgctgaac ggtatcgttg taaagcagtt
     3301 tgataatgat gcccgcaaag cggcacaatt ccggcatggt aagcacctcc tgacaaggat
     3361 agtatagcat aggcgggcgg gagttatcaa ggaaagataa gggcggggga aatataaata
     3421 gggagattaa aaagtgcccg tctccgttgg ggaggcgggc gttgttttag gtggggattg
     3481 ctttcattgt tttaccaaag tttccagtcg gagacatcgc attgaccatc ctcattattc
     3541 ccaaccgcaa ccactgttcc atctgctttt aggccgacgg tatggtcgcc tcctgccgca
     3601 atcgcaataa tatccgtcca agaactaaca ttgcactgac cacacttatt atatccaact
     3661 gcaaccacag ttccatctgc ttttaggccg acggtatgaa ggcttcctgc tgcaatcgcc
     3721 acaatgtctg tccaaaaacc aacatcacat tgaccatact cattatctcc aaccgcagcc
     3781 acagttccat ctgcctttaa gccgacagta ttagcatttt ttgccgcaat cgccacaata
     3841 tctgtccaag aactgacatt gcattgacca taggaattat ttccaaccgc agctacggtt
     3901 ccatctgttt ttaagccgac ggtatgacag agtcctattg aaacagccat aatatctgcc
     3961 caagaaccaa catcacattg accttgacca tatttatcac ttccaaccgc atcaaccgtt
     4021 ccatctgctt ttaggccgac ggtatggtag tctcctgctg aaacagccat aatatctgcc
     4081 caagaaccaa catcacattg accatcctta ttattcccaa ccgcacccac ggttccatca
     4141 gcttttaaat taacagtata ggcatctccc gctgaaatag aaggaataaa gtttttttga
     4201 atttctgaag cttgtgttct cgcatccaaa taatcgccta ccattctgaa atgaagtaaa
     4261 gcataaccca tatttccaga ctctttttct tcttttgcca aattgtattt tgcttctaaa
     4321 atacgctgga cagcgtcttt gtaatcacca gcattttcaa aggccttgga agagttttcc
     4381 cattcgccag cagcaagatg cctttcggct tgagcgtaat aaatgctctt ccaacggtct
     4441 gcggcatcgc tatagctgtc tattgcttgg aaagcagcaa tagctccttc ttcatcacca
     4501 ttagcaagca gcttttcagc tttaacataa tatggttcta agacacgttg ggcagcatct
     4561 ttgtaatcac cagcactttc aaagctttta gaagccagct tccattgacc
//
LOCUS       DAWAEA010000070         4608 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2921_length_4608_cov_2.157698, whole genome shotgun sequence.
ACCESSION   DAWAEA010000070 DAWAEA010000000
VERSION     DAWAEA010000070.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4608)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2921_length_4608_cov_2.157698"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4380..4389
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          807 a         1434 c         1419 g          938 t
ORIGIN      
        1 ctaaatctca aattcgtgtg tcgagcagct tgacatagcg gtcatggaag gcggtaaagg
       61 tgcctgcatc cagttcatcg cggatacgct ccatcaggtg gttgtagaac cacaggttgt
      121 gcataacggc cagacgcatg cccagcagct cctctgcctt gaacaggtgg cggatatagg
      181 cccgggagaa attctggcag gtgtagcagt cgcaatgctc gtccagcggc cggaaatcgt
      241 gggcgtactg ggcgttcttg atgaccaccc ggccatcatg ggtgaagcag gagccgttgc
      301 gggcaatgcg ggtcgccagc acgcagtcga acatatccac gccccgcagc gcgccctcga
      361 tcaggcagtc gggagagcct acgcccatca ggtagcgggg cttgtcctcc gccatgtggg
      421 gcatgatggc ttccaacatt tcatacatca ccggcttggg ttcgcctacg gacaggccgc
      481 cgatgccgta gccgaagaaa tccatatccc gcagggtctt ggcgctttcg atgcgcaggt
      541 ccttgaagaa cgcgccctgc acaatgccga acagcacctg atcgtcgtcg gtatggtgct
      601 gctggcaacg cttggcccag cggtgagtgc gatgcatcgc atcctcggcg gtggcgtggt
      661 cgcagggata ggcggtgcac acgtcaaagg ccatcatgat atcgctgccc agcgcgtgct
      721 ggatatccat gctgatctcc ggggagataa agcggtggct gccgtccaga tggctctgga
      781 aggccacacc ttcttccttg atcttgcgga tgccggaaag ggagaacacc tgaaacccgc
      841 cgctgtcggt caggatgggc ttgggccacg acatgaactt gtgcaggccg cccgcctcgc
      901 gtatcaggtc ttcgccgggg cgaaggtgca ggtgataggt attggagagc atgatctccg
      961 tgccgatttc cggcatttcc cggggagtca tggccttgac ggtagccacg gtgcccacgg
     1021 gcatgtatac cggcgtatgg atatcgccgt gcggggtatg cagcaagccc agccttgcgc
     1081 cggactgctt gcaggttttg agaacctcaa acgaaaactt ggatgttgcc actgaattgc
     1141 tccttaaacg cgcgactggc acgaagccag aaacgcggtg atctcttttt cataaggccg
     1201 gaaagcgggg ttttccgggc aggcggtgaa ggtcatttct ttcttgaggg tgggatgggt
     1261 cagacgaagc accgcgccga acagggcgat gtcccgtccc cgctccccgt ggccgtaacg
     1321 catatcatag cggatgggat agcccagatg gctgagctga acccggatct gatgcttccg
     1381 gccggtttcc agccgcaagt gaaccagtgc cgttccgtcc cgtgcgccca accgttcgta
     1441 gcgcagccgg gcttctttgg ctccgggcgt accggcgggc accgtccgca ccagcccggt
     1501 cttttcatcc tgcaaaagcc agtcgcacag cgtgccctcc gcaggcaggc tttccccctc
     1561 caccacggcc agatattccc gttccatatg atgaacgcgc agctgttcgc tgagccggga
     1621 ggccgccttg ctggtgcggg caaaggccac cagaccgccc acgggccggt ccagccgatg
     1681 caccagcccc agatagacct cccccggctt ctggtatttt tcccggatat actgcttgca
     1741 ctcgtcctgc aggctcaggt ctccggtctc gtcgctctgg gtcagcttgc cctgaggctt
     1801 gaccaccacc agcaggtgat tgtcctcgta gacgggctta atcatggggc gtccaccggg
     1861 ccgttgcgcc gcagggcagc accccgccgg aggtcacggc cagcgccaga tcgtccgcct
     1921 ccgcccgtcc gccataccgg ctgcacaggc agcgctgggc cacgttctgc agcaccgtat
     1981 agctgaagcc cgtggtgtag ccgttgatga ggaagaacag cggcgtgtcg ctcatggcct
     2041 gtgcgcacag atcgaccagc ccgtacagct cgttttccag cttccagacc tcgccgttgg
     2101 ggccccggcc atagctgggc gggtccatca gaatgccgtc gtaatgattg ccccggcgca
     2161 gttcccgctg cacaaaggcc tttgcatcct caatgataaa gcgggtgttt tcctccggga
     2221 tgccgctgag gtcgcggttt tccttggccc agagcaccat gcccttggcc gcgtccacgt
     2281 gggtgacgtg ggcccctgcc gccgcgcagg ccagcgtggc cccgccggta taggcgaaca
     2341 gattcagcac ccttggctgg cgcttccagc cggacgcgga acggatctgc tcctgcataa
     2401 aggcccagtt ggcagcctgt tccgggaaaa ggcccgtgtg tttgaagccc gtgggacgca
     2461 cgtagaattt cagcccgtcc agaccaatgg tccacttgtc cggcaatttt tcccggaaat
     2521 cccaatgccc gccgccggtc tcggagcggg tataggtagc ttgcgccttc ttccacagtt
     2581 caggccgggc cttgggccag atcacctgcg ggtcgggccg gcgcagcaga atcttccccc
     2641 accgttccag cttttccccg tcgccggtat ccagcacttc gtagtcccgc cattgatcgc
     2701 aaagatacat gtatttctcc ttcagacaaa cggccccggg cgggcggcgg cctgcccggg
     2761 gcaatcaggg tttaggcctt ctgaacgctc agcttggcct ttttgccatt gatgtcgcat
     2821 tccttgccgt ccactgcctc gccggaggtc agttcgtcgg ccagaacgct cttcatgatg
     2881 aagtcgcggc ccgcctcgat ggcggcgctc agctcgccgt cggcctgata gaccacctga
     2941 atatggtcgg tcacatcgta gcccgcgtcc ttgcgcaggt tctgcagctt ggagatcacc
     3001 tcgcgctgat agccttccac gatcagttcg ggggtcaggt tgcagtccag caccacggtg
     3061 acgctgccgt ccgtctgggc ggagaagccg ggcttctggg tggcttcggt gagcacgtcg
     3121 tccttttcca actcgacggg cgtgccgttt acgtcgaatt tcagggtttc gccccgggcg
     3181 aaggtgtcca ccacgtcatt gccgtccatg ccctgcagcg ccgtgcggat gccgcccagc
     3241 agcttgccgt acttcgggcc cagcgtgcgc atctggggct tgaggttgta ggtggtgaag
     3301 gcccgcgcgt cctcggtgaa gaccacgttc ttcacgttca gctcgtcctc ggccagctcc
     3361 cggaaggcct ggtcaaactc ggtgcccttc acatacaggc aggcagaggg ctgacgcacc
     3421 ttcaggttgg cgccggagcg gctgctcagg cccaggctga ccacctcccg cagggcggcc
     3481 atctggcggt tcaggtcttc gtcgatcagg gcttcatccg ccacggggaa atcgcacaga
     3541 tgcacgcttt ccggcgcgtc gggattcacg gagatcacca gattctggta aatatcctcc
     3601 gccatgaagg gtacaaaggg cgcgatgacc ttgctcagcg tcaccaacac gtggtacagg
     3661 gtgacaaagg cggcttcctt ggtctcgtcc atgcccttgc cccagaaacg ctcacggcag
     3721 cggcgcacat accagttgct caggtcgtcc accatgtcgg tgatggcccg ggcggcctcg
     3781 ggaatgtgat aattgctcag gttgtcgtcc acgtcccgga tggtgctgtt cagacggctc
     3841 aggatccact tgtccatcag ggtcagactg accttgtcca tggggtgatc cttggggtcg
     3901 aagttgtcga tattggcgta cagcacgaag aacgcgtagg tgttctgcag ggtggacatg
     3961 aacttgcgct ggccttcgtt gacggcctcg tcgctgaagc ggctgggcag ccagggagcg
     4021 ctggccgtgt agaaatacca gcggaccgcg tccgcgccct gcttatccag caccgaccac
     4081 ggatccacca cgttgcccag atgcttggac atcttgcggc cctccgcgtc ctgcacgtga
     4141 cccatcacga tgcagttttc aaagggcgcc cggtcgaaca gcagggtgga aatggcttcc
     4201 agcgtgtaga accagccgcg ggtctggtcg atggcttcgg aaatgaagtt ggccgggaag
     4261 cgcttttcga agatatcctt gttttcgaag ggatagtgcc actgggcgaa gggcatggag
     4321 ccggaatcgt accagcagtc gatgacgtcg gtgacgcggg tcatgggctt gccgcattcn
     4381 nnnnnnnnnc acttgatctg gatggcgtcc acataggggc ggtgcagctc cagatcgtcc
     4441 gccacggggc cgatggccat ttccttcaat tcctgacggc tgccgatgac gtggacatgg
     4501 ccctcttcgc agcgccagat gggcagcggc gtgccccagt agcgctcgcg gctcaggccc
     4561 cagtcatgga cgttttccag ccacacgccc atgcggcctt ccttgatg
//
LOCUS       DAWAEA010000071         4606 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2924_length_4606_cov_2.036256, whole genome shotgun sequence.
ACCESSION   DAWAEA010000071 DAWAEA010000000
VERSION     DAWAEA010000071.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4606)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2924_length_4606_cov_2.036256"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    287..379
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2581..2590
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3745..3823
                     /estimated_length=79
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1076 a         1119 c         1568 g          661 t
ORIGIN      
        1 gcacaggccg gtcttcggcg tgatgctgac ggaacggcgg aaccggcatt ttcagcggac
       61 gggccagcga atcgaagtga tgacggagga atggaacgtc cattatgagc gaaccggcgg
      121 agagatccct cgggagacgg ggcgggagag gcacttcttc ggcggcgtgc ccatgaccga
      181 atactggaat aatcagcggg aacggggcga ttttgaaggc gtgaaagcgc tgatcgacgc
      241 ttacgacgcg ctgcagagcg accgggtaaa cgacaagcag cagtttnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnc tgaaagccta tgaggggtta gccccgaaaa agctgctggt
      421 tgggcaggtg ccctttgtgg aggatgcgga gaaagccatg gaagaaatgg aaaaggaaaa
      481 gaggagataa aaagagctgc cgaagggcgg ctttttttat agaaacaaac ggaagggaga
      541 aaagagcatg gaagaaatga cggttcacga acccacggaa atggaacggc tggcgctgga
      601 acgggaacag acgctgcggc agcgggaggc ggcgctggag atgcgggaac gccgtgcccg
      661 ggcgcaggag ctgctgaccc agcgaaatct gccggcggaa ctgacggact gcctgaattt
      721 ttcggatgac gagagtctgg aaaaaagtct gaatgcgctg gaaaaggcgt ttctggaaca
      781 ggtggacaag gcggtgggcg aacggatgaa gggcgcggcg ccacgaagcg gggaaagcgc
      841 gctttatgcc ggtcagcttc ggtcggcatt gggactgaaa taaggaaaag aaaaaggagg
      901 aaaaagagat ggctaattcg atcacactat tcaaacaatt tgtgccgctg ctggacgagg
      961 tatacaagga ggccagcaag acggcggtgc tggacggccg cccggagctg gtgcaggcgg
     1021 gggcgaacgc caacgagctg gtgatcccca agatgacgat ggacggcctg gcggactaca
     1081 gccgcaacgg cggctacgtg ggcggcgcaa tgaacctgac caacgagacg gtgacctgca
     1141 acttcgaccg gggccgtatg ttcgtggtag atcacatgga caacgcagac acggcgggcg
     1201 tggccttcgg aatgctggca ggggaattta tccgcacgaa ggtggtgccg gaactggacg
     1261 ctttccgctt tgcgacttac gcgggcgcgg ctggggtcgg ttcgggaagc ggtacgctga
     1321 acgacggagc ggcggtggtg gccgcactgc gggaggccat caacgccatg gacgcggatg
     1381 aagtgcccat ggaggaccgg gtgctgttta ttacgtccgg cctgctgggc aaggtggagg
     1441 atctggacac caccaagagc cgggaagtgc tgcgccgctt ctccgacgtg gtggcggtgc
     1501 cacaaacccg cttctatacg gccattcacc agaacagcgg cacggtggta cagaagggcg
     1561 gcgagacgac cagcgacgag acggcgggcg gctacgccaa ggatgaaagc ggcaaggaca
     1621 ttaactttat ggtgatccac cggggcgcgg tgatccagtt ttccaagcat atcgcgccca
     1681 aggtggtaac gccggaacag aacccggacg cggacgcgtg gaagttcggc taccgtcagg
     1741 tgggcgtggc ggatgtgtac gagaacaaga ccgccggtat ttacgtgcat cacaaggcct
     1801 aaatgcgccc ggaaacagtg aaaggagagc cgcatggaca agcagaaagg gctggaagcg
     1861 ctgcggcttc ggctggaaat ttcccatgag gagcaggaca cgctgctgac ggcgcttttg
     1921 gaggatgcgg agcagtatgc gctggggtat accgggcgaa gcgtcctgcc tgccgcgctg
     1981 gaaggggcgg cgctggaact ggcgatcctc cgctaccgcc gacgcggcat ggagggggaa
     2041 accgtccatg gagaaggcgg cttacagacg actatggaag ggctgccttc ggcgttggaa
     2101 aagctgctga atcgctaccg gagagcgaag gtggtctgat gggactgagc cgcggaagaa
     2161 tgagaagact atggcttcat cagcccaaag cacagcggat cgcggacggt atggaaccga
     2221 cggagctgaa agaaggtgtg gggatcgcgc tggaggcggc gggagaatgc cggttccgca
     2281 tcgcggcacc gccggagcgt tggctgaccc atcagcgggc ggttttgaaa agcatctagg
     2341 aggcatggat gggactggaa aaagtggggg cggcgatagc cgccggtgca gcgagcgccg
     2401 tggaacggtt gtgcatggaa actgcggaaa atgcgaaggc gttatgtccg gtggaatccg
     2461 gagaattgaa gggcagtatc gcgccgcagg tcatgggccc ttcgggaagc gtagccgccg
     2521 gggcgggcca tgccgcctat gtggagctgg gaacctggaa aaaggaggcc cggccttttt
     2581 nnnnnnnnnn aaaaagcggc ggaggaaatc gcccgggaga tcagaaacgc gattcgggag
     2641 ggatgaactt gagcgcggaa ggagagaaac ggcgcattct ggaagcactg gggagcatcg
     2701 aaacgctgaa aaacgtcagc cgcagctggc cccgccagca ggcggcgctt ccctgcgcgg
     2761 tggtgacatt ggccggggag cgacgggcag acgaacggga tggggccgag tatctgacgg
     2821 aaacggaata ctatgtgcgc tgctttgccc ggacgggaac ggaagcggat gaactggctc
     2881 cgaaaattcg gcgggagatg gaaaaactgg gctatcagcg ggaatttgcc tgggaagaaa
     2941 gcagtgaagc ggtttttcag cgggtggaac ggtacaaaaa gtggatcgga taaaagagag
     3001 gagaatgaga aaatgagcca gaaacgagcg gcaacgggat tcaagggcat cgcgctggcc
     3061 ccggtgacgg agaacacgct gaccagctac aagacctcgg cggcggaaag cctgccctac
     3121 gcgggcagca tgagccgcac ctccaaggag agcagtaccg acctgtacta tgacgacgac
     3181 ctgtacgccc agatcaagaa cgtcatgggc gaggacgtgg agctgcacat ggcggaggtg
     3241 ccgctggaac gcatggctgc gctgggtctt ggcacctatg acgaggaaaa cgacacgctg
     3301 gaggcggact tcaacgccgc cgggaaggag tacgccctgc gcttcgtggt ggacacggtc
     3361 agcggcctgc ccttctactt caactaccgg ctgttccagc tgacgggcat caagttcgat
     3421 aacttcacca ccaagcagga cagcgtgacc gtgtgcgagg tcatcatcac cggcgtgttc
     3481 aagcggccca acatggcggg ccttgcgccg tgggcggtga tgcagctcaa ggacgacaag
     3541 agcaatcagg ccaagtgcac ggccttcatc acggcggcgg agacgaagcc ggccacttaa
     3601 aaaagcaggc cgggggacgg aaaaccatcc cccggcgctt tctaaggacg aataaaagga
     3661 ggccccatga cgcgctcggc agagtactgg aaacggcggg cggaagccaa catggacggc
     3721 gtgcagcggg gcgcggagca gcgcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggctggg ggcacggctg
     3841 gaacgggaga ctgccgctca gcgggaatgg acggcctcta agagctacca gagcgcccgg
     3901 aagatgatgc gggacatgac cggcggggtg gtgagccaag ccgtgcccga cctgcagggg
     3961 cttctgcggg ccatggacac cgcgtggtcg ggccggaact actccgcccg cgtctggcgc
     4021 aacacggacc agctggcgca gatgctggaa gacgagatcg aagccgcctt tttgagcggc
     4081 aagagcgtcc gccggatggc gaacgccatc atggatcgct tcggcgtggg ctaccgggcg
     4141 gcggaatgcc tgatgcggac ggagaccagc tacgtgcaga accagacagc cgcccaaagc
     4201 taccgggacg cgggctgcac ggactacgaa attctgaccg ccagcgaccg ccgcacctgc
     4261 agccgctgcg ccgcacaaaa cggcaaacgc taccccttca cggccatgca ggcgggcgaa
     4321 aacgccccgc cctttcaccc gaactgccgg tgcacaattt tgccggtggt aggggaagaa
     4381 gagcggctgg cgaaaaaggc cgggccggag gaactgggct ttgccgttgc aaagaccggg
     4441 gaaagtggta taatgtcaga gaagaaagtc atcatggatg ccatttcttc agaaaccgtt
     4501 accactaaaa tcaatattgc caagcaggag agacatataa aaaataatcc ttcctacatc
     4561 gagggaagaa gttatcttta tggatcatta gcagacgctc aacgtt
//
LOCUS       DAWAEA010000072         4575 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2947_length_4575_cov_2.141814, whole genome shotgun sequence.
ACCESSION   DAWAEA010000072 DAWAEA010000000
VERSION     DAWAEA010000072.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2947_length_4575_cov_2.141814"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    238..247
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2202..2367
                     /estimated_length=166
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3016..3071
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          865 a         1333 c         1298 g          847 t
ORIGIN      
        1 cgatgaggca tggatttgcg cctgcaggag cactgggggc tttcctcggg caaacaatcg
       61 agcgaccccg ttccccatca tattccccct gtcccccgca cacactatcc ccatcccccg
      121 catatcctcc tattgcccgg cacaggtaaa ctctaaggaa aagaggtcat tcgctatgca
      181 tccgtatcgt gtaggtatcg tgggcgcgac cggaatggtc ggccagcgct tcactctnnn
      241 nnnnnnnccc ctggtttcag gtgacctgtc tggccgcgtc cgcccgcagc gcgggaaaac
      301 cttaccggga agcagtggaa ggccgctggg cgttcaagga tcgccccatt cccgctaacg
      361 tggcggatat gaccgtgctg gacgctgctc aggtggagga agtggcctcc cgggtggatt
      421 tcgtcttctg tgccgtggat atgaagaagg acgagatccg cgcgctggag gaacgctacg
      481 cccggacgga aacgccagtg gtgtccaata acagcgccaa ccgcggcgtc agcgacgttc
      541 ccatgctgat ccccgagatc aacggcgacc acgcggcggt gatcgaggct cagcggaagc
      601 gtctgggcac gaaaaacggc ttcatcgccg tcaagcccaa ctgctccatc cagagctacg
      661 tgcccgccct gtggcccctt ttggattttg aacccaagaa ggtcatggtt tgcacctatc
      721 aggccatcag cggtgcgggc aagaccttcg agcgctggcc ggaaatggtg gataacgtga
      781 ttccctacat cggcggcgag gatgaaaaga gcgaaaagga accgctgaag attttcggcc
      841 atctggaaaa cggccagatc gtcaacgcat ccgaaccggc tatcagcgcc cagtgcctgc
      901 gggtcgcctg ctccgacggc catatggcgg cggtcagcgt ggagttcggc aaaaagccct
      961 ctctggagac cattttggaa cgctgggcca actgccatcc cgcaacggag ggattgggac
     1021 tgccctccag cccggagaaa ttcctgacct acttcgacga ccccgcccgg ccccagaccc
     1081 gactggatcg cgacctttac ggcggcatgg gcatttccat cggccgtctg cgggaagata
     1141 ccctgttcga ctacaagttc gtctgtctga gccacaacac cctgcggggc gcggcgggcg
     1201 gcggcgttct cagcgccgaa tacctgtgca agctgggcta tattccccac aaataaccgc
     1261 ctgacaggag gaaaaacatc atgatacaac ctctgtttcg cggcgcggcc accgcgctgg
     1321 tcacgcccct tcgggatggc aaagtggacg tggacgctct gcggcggctg gtagagcagc
     1381 agctggaaaa cggcgttgcc gcgctggtca gctgcggcac caccggtgag ccctccaccc
     1441 tgtcgctgga agagcgggag ctggtcatcc gggagacggt gaaagccgtg aacggccgcg
     1501 tcccggtcat ttgtggcacg ggggccaact gcacccagac cgtcatcgac aacgagcgcc
     1561 gcttccgcga cctgggctgc gcggcccagc tggtggtcac gccctactac aacaagacct
     1621 cacaggaggg cctgtacgct cattttatgg ccattgccga gcatacggag ctgcccatca
     1681 tcctctacaa cgtgcccagc cgcaccacgc tggaaattct tcccgatact ctgcggcggc
     1741 tgatcccctc gggccggttc atcgccctga aggaagccag cgccaatctg tcgctggtgc
     1801 tggaaaaact ggacgccatt gacggacggc tgaccctgta cagcggcaac gacgacctga
     1861 cctatcccct gctggccatg ggcgcgcagg gcgtgatctc cgtagtgtcc aacgtgctgc
     1921 ccggggaaat ggccgcgctg tgtcaggcgt atttcgacgg cgacgcgcag gattccctgc
     1981 gccgccagat tcgcctgatg cctttcatcc gtgccctgtt ccgggaggtc agccccattc
     2041 cctgcaagtg cgccatggaa atgatgggcc agtgctccgg ggagctgcgg ctgcccctga
     2101 tccccgccgc agaggaaacc agaatggcgc tgaaaaagtg cctgacaggg cttggatatc
     2161 tgaaaagcta aaaaccgagg gacagagcca tgctctgccc cnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnatg ggccaggaag tgacggccgc agccgcagag
     2401 gctcaggtgg agatcgtctg cggcgtggac gtggcctata cgggtcagcc gtcggattac
     2461 cccatcgtga ccgctttcga tcaggcgacg cagaaggccg acgtgctgat cgacttttcc
     2521 cgtccctcgg cgctgccgga gcttttgcag ctggtacgga aagaaaaaat gccggccgtg
     2581 ctgtgtacga ccggttataa cgaggcggaa ttgcagatga tcgaggaagc cagccgggaa
     2641 atccctgttt tccgcagcgc caacatgagc cttggcgtga acgtcctgcg ccatctggtg
     2701 caggaggccg cccgcgcgct gggtgaggac ttcgacgtgg agatcgtgga gcgccatcac
     2761 cgcatgaagg cggacagccc cagcggcacc gcgctgatgc tttacgactc ggtgaaggaa
     2821 gccacggaaa ccctgcgcca gccggtattc ggccgctatg ggcgcaccgc ccagcgccag
     2881 cccggcgaag tgggcatcca cgccgtgcgc ggcggaacca ttaccggcga acacgaggtc
     2941 tgcttctatg gcgacggcga agaactgatc ctgacccatc gtgcggaaaa acgttctctc
     3001 tttgcccggg gtgcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn ntcctgatgg acaagtctgc cggaaagtac gacatgcagg acctgatcga
     3121 agaaatgctt cgttaaccct cggcggacgg gccgttccag tcgtccagat ggctctgcag
     3181 ggtggacaaa atctcatgca ttacatagcg ctggccgcgg cttagaccgt cggggccgct
     3241 gtcgttacgc acggtattca ggctgtcgca cagaaggtaa tccacgccca cgtgcagggc
     3301 gttggccagc gctaccagtg tttccagact ggccttgcgg gagccgcgct ccagatggcc
     3361 gatgaaggaa gtagtcacgc ccacctgccg ggccagctgc tcctgagtca tgccctgctc
     3421 cacccggcgc ttgcggattc tttttcccaa cgcgaaatag tccatatttg acgctccttt
     3481 cgttcaaaaa cttcgttcgg aaaaatattc catttttcga catatttcgt ggtaatttgt
     3541 accatcatct ttagaaaaaa acctccgttt catttttcgc tttgcggggg atgagcggag
     3601 gttttttgcg tttgttaata ggggcgttcc tttccgaaga agcacacggc taccgtgccc
     3661 gggccgcagt gcgcgccgat cacggggccc acatagcggg tggaaatatt gcccagaccg
     3721 gggatcttct cccggagcag ctgtaccagc cggtcggcat cctcgggcgc atcggcatgc
     3781 agcacataca tttccgcctc ggcggggtcg tcgatgttct gagcggccag ctccgccagc
     3841 gtgcgcagcg cctgcttgcg gccccgcacc ttatccgtgg aaagcagctt gccgttcttc
     3901 ccctcggtga tgatgggctt cagatccagc atggtgccta cggtagcggc cacggcgctg
     3961 atgcgtccgc cacggcgcag gtaggtcaaa tcgtccaccg taaagaaggc ctgcgaacgg
     4021 ctcttgtttt cttccagcca cgcggccact tcgtccatgg tcttgccctg ccggtacagt
     4081 tcatgtgcct tcagcaccag aatgctctgt ggcagcgaaa tgctcagcgt gtccaccaca
     4141 atcatgcggc gctcgggata acgctgcaga agctcctccc gggcggcgta gatattctga
     4201 atggtgccgg acagctggct ggaaaacgcc acgaacagga tatcccggcc tgcggcgaac
     4261 acgggctcga agtattcctc gtaggcggcg gtaggcagca gggatgtgac cggcgcggcg
     4321 cccttgcgca tggcgtcgaa gaactccttt tccctgccgt tgcggcccag atcgtcaaag
     4381 tattccttgc cgtccaccat gtagggcatg tagaccatgt tcagttccag ctcgtctaca
     4441 aaccgaaacg gcagatcgga gtccgaatcc gtgaagaatg cgtatgaatt catagttcct
     4501 ccttgtgtca gatgattcca tgaaatgata tgatggcgta taaatccaat gctattgtag
     4561 cggatttttg tctga
//
LOCUS       DAWAEA010000073         4516 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2991_length_4516_cov_3.384219, whole genome shotgun sequence.
ACCESSION   DAWAEA010000073 DAWAEA010000000
VERSION     DAWAEA010000073.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2991_length_4516_cov_3.384219"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4272..4281
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          925 a         1282 c         1322 g          977 t
ORIGIN      
        1 caaacggcgt gccctgcgcg aagggcatgg actgctttaa ctcttttgaa accgccctcg
       61 cgcgggcaga ggattctgag ccatgccatg ccgacatatc tgttttccgg cgtaaactgc
      121 cgggaatctc ggctttcaca acagtgaaat tccagatatc gtccacaagg cagggttttg
      181 tcccaaatat cgtccaacag cgttaaactc cgcaagcatt cgtcacagcg tggaaaaggc
      241 cgatggctct gcctctttcc gagtggatga tgggctgttt gtaacgaacc ccgtatcgac
      301 ggttatatat ttttctccat ccagttcatg tctttatgag tctgggtatc ctccatatca
      361 tcccccaaat cgccgtggat ttcaagaata tcgtctatac atatcccatt gccggtgtag
      421 gtttgtcaaa catattggac aatgaactca atgggggaaa gccaatggag gggtctcata
      481 ggaaagttgt tggaacggga atgatgaacg gcgagctgct ttgcaaagtc atccagagag
      541 tagaagtcgt ggcaagaata gaagcgcttc tgatcctcac ggtggctgcg ctcgactttg
      601 ccgttgtgcc gcggcgtata cgggcggatc agcttgtgcc ggatgcccag ttcggcggct
      661 gtcttttcaa acagcgtctg taaatcgcgc ttgctgttgg aaaagcgatt ggtgaactca
      721 aaaccattat ccgtctggac gcattccacc cggatgccgc ggcgtgcgta ccatgccgtc
      781 agcttgcgaa gaaaatccgc tgaggagtaa gtagattgct ccggataggc ggcgagaaag
      841 cgcagccgcg tgaactcgtc gatggcggtg tactgaaaca ggcgaagatc agggtctgcg
      901 atgcagctgc gcggaacgac ctttacgtcc acctgaacgc gctctccggg atgggtcatc
      961 tgctcataag gctttggctt gtaagccttt ttgggttttg ggtccggaaa catccccagc
     1021 cttcgcatca cgcgaaacag gctttccgga caacgtgtat agccgcgccc tcgcaaacga
     1081 tgccacagtt cgatcatccc cagggttgga ttccgacggc gcatgtcacg aatgagcttc
     1141 agctcggctt ccgtgtgttg attcggatga ccgtgcgggc gtctggactg gcagccgagg
     1201 gacgccacac ttccatccca acgctgcttc cagaagtaga tgtacgaccg gcttttgttg
     1261 tactttcggc tggcacggct aacgccgtat ttctcggcat acttcatcag cgattgccga
     1321 tacgccatgt cttgtgttat actcttgctc atgagggata tggtctcctt tcgccgagtt
     1381 gtgatgtggt aactcaactt tagcatatga gaccctatct ctctttcctt ttttgtgttt
     1441 gtccaagatg tattgtagtc ctacaccttc ctttttgggg atcgtcccct ccccgttgca
     1501 ggggaagggg gtggtgtggt atgatggagg gagaaaagaa tgggggaggg aataagcgtt
     1561 gaaaagaagg cggagacggg gactttcctt tggcagcgtg gtaatgatcg ttttgaccgt
     1621 ggccgtgctg gcggggttcg ggacgatgat cccccggttc accggcagcg aaagctttga
     1681 ccagaacgcg gcgcagctgg ccgtggcgct ggatcagacc atcgtgaatt tctcctccaa
     1741 tttgggagcc atcgcttccc agaaggaaac gacgccgccc gcgcccctgt gggcgacggc
     1801 cacccccatg cccaccgcca cgccggttcc tacggcggtg cccaaggcaa gctttcggct
     1861 gtgcgccacg ggaatgctga attggagcgc atccattcag aaatccgtgc agacggacag
     1921 cggctatcag tacgccgacg tgctcagccc gctgctttct cagctgaacg ccgatgtgac
     1981 cgtagcgacc ctgcgcaatt ccctgatcgc cgatgaaaaa atctccgacg gcaacgcgga
     2041 tgcttcgctg gcggccgcgc tgttccagag cggcttccgt gcgctgaatc tggggcacct
     2101 caacaccttg gattacggtc tggacggcct gtggaagacc cagagcaccc tgaccgacgc
     2161 gggggttctg cccttcggag cgtacccgtc cgcccagcgg cgcagcacgc tgacgctgac
     2221 caacgcgggc ggcgtgaagc tggccctgct gagctatcag agcggcgtca gttccgaggg
     2281 ccggaaggcc gtgtctgccg acgaccgggc ctacgccgta ggcgagctgg agctgtccgt
     2341 cattcaggaa gagatcaccg ccgcccggaa cgcgggcgcg caggtggtgc tggtatccct
     2401 ctgctggggc aaggaaaacg ccacaaagcc caccaacgaa caaaaagagc tggcgcaggc
     2461 catcgccgac gcgggcgcag atattctgct gggcgtgaac cccaaggccg tccagacggt
     2521 ggagatcctc accgccgacc ggggcgacgg ccgctatcac ccggtgctgt gcgcgtacag
     2581 tctgggcagc ctgctgacct ccgaacggca gcagcgcacc aatctggcgg gcgtgctgct
     2641 ccacgcgacg gtggaatatg accctgccac cgacaccgtg gccttcgacg ggctgaccta
     2701 cacgcctacc tacaactggc gcaccaagga aaacggcgtt taccgctacc gggtctaccc
     2761 cagcaatctg gccgaatatc cggcggacgt gaacaaggat cagctgaaca tcatggccaa
     2821 gtgccggaag atcattcagg acgtgatggc agacagcggg tttacggaac aataatatta
     2881 taataggaaa agaaggaatc tgaaatgaaa ctgctggccg tgggaaacag cttttcgcag
     2941 gacgcctgca cctttcttca tcagacggcg caggcgcagg gactcgatct ggaagtgacc
     3001 aacctgtata tcggcggctg ctcgctggaa atgcattacc agaacctgct ttccggcaag
     3061 gccgactatc agtgtcagca gaacggtcaa tttgacggac ggatgatctc actgccggaa
     3121 gcccttgcca tggagccgtg ggacgtgatc gtcacccagc aggccagcca tttcagcggt
     3181 tggatggaca gctacgagcc ttttctgacg gagctgctgg aggatttccg caaggcccgt
     3241 ccgcaggcgc ggctctatct gcacgaaaca tgggcatacg aacggagcag cgatcacggg
     3301 gcctttttcc gctaccattg cgaccaaatg gaaatgtatg accggctccg ctactgctac
     3361 cgtaccatgg cccgtcagca cggcctttcg ctgatcccca gcggcgatgt gatccagcgg
     3421 gcgcgcacga aggagccttt ccgggtgcag gacggcggac gttccctgtg ccgggacggc
     3481 ttccacatga gtctgagtta tggccgctac ctgctggcct gcgtgtgggt ggggcggctg
     3541 tttcacgtat ctgtggcgga caatcccttt attcccgacg gcgacgaggc cgtagagccc
     3601 gctttactgg cgctgctgcg gcagacggcg gacgaagtgc tggcggagca gcgcagtcag
     3661 gaataaggcg ctttccgttt tccgccactc cgaccattaa aaccccgcaa ggcgtaaggc
     3721 tttgcggggt ttctctatcc atttttcatt tcccaatatc tcccttttga ctcccgtttc
     3781 tgcacccttt gggggtcaaa acgggggcaa aatttttcaa aaagcgccgt tatggacggt
     3841 tcaaaggttc aaacttgccc gccttcggcg gccgagatac ggaacataaa gaaagaagac
     3901 tgcctgacgg cagccttctt tcgtataggt tatttttccg ttagttggcg cgcaccttga
     3961 tgctgggcca atcatagatg ccaacgggcg tcatgttgaa gtcctccacc tgggcggaca
     4021 ggatgacggt ctcgttgcgg ctgttcagat agcagccgac tggatcctcc aggtacagga
     4081 tctccgcagc gcgctggtag gccttttcgc gccattcctg atcggtggtc ttggcagcgc
     4141 cgttgagcag ctcgtccact tcggcgttgg agtagaagcc gtagttgttg ctgttcttgc
     4201 cgtcttcttc agtagcgaag cggcgcttca gaccctcgtc gccgtccagc gtgatgtagc
     4261 cggcgcccat cnnnnnnnnn nccagtcggg gaattcttcg ggggtcaggc cgttccacgc
     4321 agcggagaag gtagcgcggt caaccacgac cagattgcac ttgatgccca cttccgccag
     4381 ctgagcggcg gcgatttcac cggcctgcac acccttgttg tacacgtcgg tgcacatgag
     4441 gttcatagcc agccaagctg ccgaccttgg agacaaaagt gttgagcgta tttttaccaa
     4501 agtacccatc cggcgt
//
LOCUS       DAWAEA010000074         4514 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_2993_length_4514_cov_1.856919, whole genome shotgun sequence.
ACCESSION   DAWAEA010000074 DAWAEA010000000
VERSION     DAWAEA010000074.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2993_length_4514_cov_1.856919"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    306..361
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4044..4053
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          871 a         1246 c         1396 g          935 t
ORIGIN      
        1 gggctggccg tcctgttttt tgagcttttc aggagccatg acggcaggac gataatcggc
       61 aggagccgcc gcctgaatga taacatcctg agaagcacag tgggtcatca ttgcgtcgta
      121 gagattttgg gtagacataa ccggcacgac cgatactccg gcaggagccg tcaaatgaac
      181 cgggccggaa accagcgtga cagaagcgcc gcgggcctgt gcggccgcag caatggcgta
      241 acccattttg ccggaggaat cgttggtcag gtaacgaacc gggtcgatac gctccatcgt
      301 gggacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 ntccacgatc tgcgcaggct cgctcatgcg gcccgccccg gtgtcgccgc aggcaagaaa
      421 gccgctttcc ggaccgacaa agtggacgcc gcgggcctgc agggtacgca gattggcctg
      481 tgttgcctcg gccgtccaca tgcccgtgtt catggccgga gccagcagaa tgggcgcttt
      541 ggtggccagc agcgtggtgg tgagcatatc gtcggcaatg ccgcaggcca gcttggccat
      601 cagattggcg gtagccgggg ccaccagaaa cagctcggct ttctgagcca gcgagatatg
      661 cttcacgtcc cagctttcgg tgcgggtaaa ggtatccaca cagacgggcc gcttgctgag
      721 ggtctcgaag gtcaggggag ccaccagacg ggtggcgttc tccgtcatga taacatccac
      781 ctcatggccc agtttcttca gccgggagac cacctcgcag gccttatagg ccgcaatgcc
      841 gcccgttacg cccatgacga tgcgcatgga attactcctg ttcctccggg tcgcgctcgt
      901 aggtgagcag accgcggttg atttcttcga tagcgatctt caggggcttc atgtccttgg
      961 ggtccaccag aggctgagcg ccgtccacca gctggcgggc gcgcttgctg gcttccacca
     1021 cgagggtgta gcgggaatcc actttttcag acagttcaac gatggtagga ttgacaatgg
     1081 ccatggaatg ttcctccttc aaataatggg aagtgatggg cagacggacg aatcagtcgt
     1141 ccagaatatg gggatggaag cggctggtgc gctgcttctc ggcgcggaca atggattgca
     1201 gctgggtata ggccagctcc agattgtcgt tgatgatggc gtactggtaa tgatccacct
     1261 tgcggacttc gccccgggca ttgttcagcc gacgctcgat ctccaccggg tcgtcggtat
     1321 tgcgggtatg cagacgcacg cgcagatcct caaagctggg gggaaggatg aaaacgctga
     1381 cataatcgct ggcgttttcc ataacggaaa gcgcaccctg aggatcgatc tccagcacca
     1441 cgttcaagcc ctcggagatc atcttttcca caggctcccg cagggtgccg tagctgtggc
     1501 cgtgcacgga cgcgtgttcc agaaaggcgt tttcctccac caactgggcg tacttcgcgt
     1561 cgtcgatgaa gtaatagtga acgccctcga tctctccctc tcggggctga cgggtggtga
     1621 cgctgcagct gagccggaag cttgggtcgc tgctcaaaag ttttttgacc aaagtgcctt
     1681 ttcccgcgcc ggacggcccg gaaataatca gcagcatccc cttacgtgca atggaaacag
     1741 ccatggatca agcctctctt tcctcatctt tggagttggg gtctttatcg gtaagcctgc
     1801 ccgcgatggt ttcgggctgg atggcggaaa ggatcagatg atcgctgtcc gtttgaatga
     1861 cggcgcgggg gcggcggccc tgtgtgcagt ccaccacccg gtgcctttcc cgcgcgtcct
     1921 gaatgagccg cttgaccggc gcactgtcgg ggctggcgat ggcgatgata cgatccgccg
     1981 ccaccatatt gccaaagccg atgttaatga gtcgaagcaa tgaaagttcc tcctgtcgga
     2041 agcgtcagac tacgttctgc acctgctcgc gcagcttttc aatcacgcat ttggcggcga
     2101 ccacccgccg ggcgatctcg ctgtcggaag ccttggagcc gatggtgttg acctcccggt
     2161 tcatttcctg cagaaggaag tccagcttct tgcccgcttc ctgggcggtg tgaatcgtgt
     2221 cggcaaactg ggcaatgtgg ctttccagcc gactgagttc ttcgtcgatg gcgcagcgat
     2281 cagccatgat ggctacttcc tgcgccacgc gctggggatc ggcctcggcc acctgccatt
     2341 cggccagccg ggcctgcagg cgtttgcggt aatcctccgg cacctgcggg gcgcgctggg
     2401 cgatttcatt gcgaagggtg gaaagcgcgt ccaaattgga aagcagatcc cgggcgaggg
     2461 cttcgccctc ggtgtggcgc atccgcagga atgcgtccac ggcttcccgg gcggcttcca
     2521 gcgccagcgc cgtgacggct tccgcgtcct cgtccgcctg acggagggaa taagccccgc
     2581 tcatggagag cacctcggcg gcatcgggtt tcttgcagaa gtccagaaga tcttcgcaat
     2641 catgccacgc cttgcggaag ctgtggagaa gctcccgatc caacaccacc gtctgagcgt
     2701 cctcccggtg attggcgtag ttgataaaaa tgtccacatg gccgcgccgc acgtcgcttt
     2761 cattcagaaa ggaacggaac gcgtcctcca gaaaggaaag atttttggga gcgcggaagg
     2821 acaaatccag aaaacgatgg ttgaccgcct tcaattccac ggtgatttcc cgaccgtccc
     2881 gccgggcggc gccacggccg tagcccgtca tgctgtccat ggggtgcgcc tcctttcgat
     2941 gtaaccttta ttataccatt ttatgaagcg ttatacaagg tttgactttt agctttcatt
     3001 tggggtatat atgagggaca agtaaggaaa ggcaggggca aaccggcctt ttcgaaggac
     3061 ggaggaaaag agatgaaact gaactacaag cggacgctgc tggtgggctt cgcgttcctg
     3121 tccatctgcg cgttctggca gctgtacgac aacgtaatcc ccctgatctt aaaaaacacg
     3181 ttccatatga aggacagtct ggtgggcgta gtgatggcca tggataacgt gctggcgctg
     3241 ttcctgctgc cgttcttcgg caagctgtcc gacaagtgcc acacgaagct ggggcggcgc
     3301 acgcccttca ttctgggcgg cacggctgcg gcggtcatcc tgatgaatat cctgccctat
     3361 gcagacaatc atgaaaaact gctgctgttc gtgatcccgc ttgggctgct gctgattgcc
     3421 atgggcacgt accgttcgcc agccgtggcg ctgatgccgg acgtgacccc caagcccctg
     3481 cgctccaagg ccaacgccat catcaatttg atgggcgcac tgggcggcgt gttcacgctg
     3541 ggcgtgacgg ggctgctggt ccacgcgggc gctacgggcc ggaacgacta tacgatgctg
     3601 tttctggcgg tgtcgctgct gatggcgatc tccgtgatcg tgctggtgct gacggtgcgg
     3661 gaaaacaagc tggctgcgga agtggcggcc atggagaccg acgaggaaaa agaggcggag
     3721 gccgaggaaa agaaggaaag cgggaaggcc ttcggcgaac tggccccgga cgtgcggcgc
     3781 agcctgtggc tgatcctgtt ctccgtggcg ttctggttca tgggctacaa cgccgtgacc
     3841 tcggccttca cccggtacat gcaggtacag tggggctatg acatcaaggc ggcgtccctg
     3901 tgcctgatgg tggccaccgt gggcgcagtg ctgagctatc tgccggtggg cattctgtcc
     3961 agcaagttcg gacggaaaaa gctgatccag gcgggcgtga tcctgctggc ggtctgcttt
     4021 gccacggcgg gtctgtttac cgcnnnnnnn nnnagatcag caagagcggc gacatgggca
     4081 agtagacggg atattactac actttctcca tggctgcgca gatcattacg cccattttga
     4141 gcggtctgct gctggaacac gtgggctatc agaccctgat gccctatgcg acggtgatgg
     4201 tggcgatctc ctttgtgacc atcagtctgg cccggcacgg agacaacaag cctgagccgc
     4261 ccaaaagcaa actggaagcc tttgatgtag gagatgactg aacatgacgg tggtatggtg
     4321 gattcttgga attttggcgg tactgtttct ggtatatctg tggctgatcg ccccggcggc
     4381 aaaaaagccg gacgacagtg cgctcaaggg atggctgtat gcgcatcgtg gattgcacga
     4441 cggcaatcat caggtggcgg agaacagcct ggaagccttc cgacgggctg tagaggccgg
     4501 gtacggcatg gaac
//
LOCUS       DAWAEA010000075         4486 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3017_length_4486_cov_1.763033, whole genome shotgun sequence.
ACCESSION   DAWAEA010000075 DAWAEA010000000
VERSION     DAWAEA010000075.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4486)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3017_length_4486_cov_1.763033"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4093..4204
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          987 a         1218 c         1344 g          825 t
ORIGIN      
        1 gtgtcggggg agaaggcaaa gtaggtgaac tgacccgcgt cagcgtccgc cgtctgatag
       61 aacaggaagc cgttttcgtt ttccacgtcc atggcggtgt actcatgacg gaagatttcg
      121 ttgccgtccg cgtccacgcc ggagatcgtg ttgccctcga cggtcatctt cgccacgccg
      181 cccagatagt agcagtcaaa ccgcatactg tcgggatcag cttcgtaagc gtcgatggct
      241 tcctgaccat acaggtcgcc ggtgcacata tacagcagat aggaaacggt gtcctcggcg
      301 gcttcttcgc ccaccagcgg ggtgacgttg ttcagccacg cgtcgtgata ctgttcttcg
      361 gacagaacgg ggaacagctc cacgaaggtg ccctgaatct ggctgagaaa atcagcgtct
      421 tcagccgtcg caaccgcgct gaagctgaac aggcatacca gagaaagcag cagagaaagc
      481 cattttttca tgatggaaaa ccctccttta acaaggttag tttaatttaa ccgcatggga
      541 gcataccata tttgacgtga aaaaactatt ccgttttggt ctgaatagcc cattttgttt
      601 cgttgcctgc ggcacagcgg taggcgctgg gggtaacgcc gtagcgggcc ttgaaagcgg
      661 cggaaaattt gctggcgctg tcgtagcccg cgtccatggc aatctccacg atgtgtttcc
      721 ggctgcttaa aagctccacg gccgcctgtt ccagccggta ttcccggatg taatggtaca
      781 ccggcatccc gtagacctga cggaaaagcc gctgcaaatg ggaaacggaa atctggtgct
      841 cctctgccag ccgggccaga gaaacatagt tgtttcgatt cgtcagcaga tgatcccgca
      901 aatggcgggc cagccggagc tgctccgccg agcagtattc ccgggaaagc tcggcccggg
      961 ggatggtctc caacaggctg aacagttcca gcgccttcag cgtcagcgtt ctgcggtcaa
     1021 aatagggcag attttcaaac agttcccgga aaacgtgttc gcaccggggg cctgccccgg
     1081 ctacggcata ccattgggag gccagcaggt tctccttgag ggcagaaaag ttacaggcca
     1141 gcggggcaac gttgcggcgc atccacgctt cggcgggggc gcagtccacc gtgatgcaca
     1201 cgccctcata atagcccagc ggaaaatcgg aatgagagtg cagtccgtga acgccgtcaa
     1261 aggtgctgac cgccatatcg cccggcttga gcaccgccag atcccgggaa gaaaagtggc
     1321 tttcgaagcg gccgttaacg cagaagttga tctccagccg gtcccgttgg ggtcggtctt
     1381 cggggaaccc atgggtttcc agctgcgtca gcatgaccgt tacgccgtcg aacagcggat
     1441 agacccgcat ggttccctga ccgtccgcac agggtatgtt ttcctgctcc acgccgctga
     1501 aaccgtctat gatacggatg tagctcacga tttttctcct ttgtgctttt tatcgggcgc
     1561 cttccagctg aagcagccgg gccttgcgct ccacgccgcc tgcgtagccg gtcagactgc
     1621 cgtcgcttcc caccacccgg tggcagggaa tgaggatgga tatgggattg tgccccaccg
     1681 cgccgcccac ggcctgcgcg ctgcgaacgc ccagccgtcg ggcaatttcg ccgtaggtca
     1741 tggtttggcc gcgaggaatg gtcaaaagaa tgttccacac cgtctgccgg aagggggaac
     1801 cctgcggatg caggggcggc agaaagcccg ggtcttttcc actgaaataa acgtccagcc
     1861 agcgagcagt ttcggtcaga acgggcgttt ctttttcctg atagtcaacg cccaaaaaag
     1921 acggaaaata tttttgtgcc tcgaaccaga gacctgtcag gccggtttca tctgccgcca
     1981 gcaagatgga gcccaatgga gaagcgtaat gggtcagaaa aagcatatgc cgcggctcct
     2041 tttcaagaga attgagggaa aaggaaagaa atacgctttt gttatatcag ccctttttcc
     2101 gatttgtcaa gcgtatgccg ctgaaagacg gtcaggataa tttggcaaaa acggggaaga
     2161 atcacctgcg gaggtgatga aatcatgagt cccaaggaac tgttgtatct cgaagatgcg
     2221 ctgggccatg aaaaatttct gaagacccag tgccagcagg ctgcgcaggc cttgcagaat
     2281 ccgcagctga agcagtgcgt ggcccaatgg gaggaaaaac acgcccagct gtttcagaag
     2341 ctctacggcc tgatgtaagg gaggaaaagc aatgaacgcg gacaaggaga tcatggaaaa
     2401 cctgctcaac acggtaaagg gcgtatgcga cctgtatatg cacggcacca tcgagtcctc
     2461 gacccagaac gtgcacgccg cctttgacga cgcgctggaa gagagcctgt gtatgcagaa
     2521 cgaaatttac aatcagatgt cccagcaggg ctggtatccc atggaacagg ccccgcagca
     2581 gcagatcgac aaggtgcgcg acaagttcgc caacgcctga caaccccaga gaacggaagc
     2641 ccgccggcgt cgcgctgcgc gggcttccct tttgcccgca gaagtctgtc gaaaggacgc
     2701 ttaccttgac ggaatggggc ggaagtgtta tctttattct cggagtattg gaacctgaag
     2761 aaggatcaga acggttctgc gctgggcaac aggcgtggaa gaaacggaga ggtgaatccc
     2821 accgatgagt acccttaccc ccggcccccg caggcgttcc cgccgggcgg aacgggcggg
     2881 cgatatgttc cgtccggaag aacaggccgg aatggaaacc tatcaggctg aaaatgaaaa
     2941 tccgccggag gatgcgtccg ttttttcgca ggacgcgccc cagatggaga agccggactg
     3001 gtcagcttat taccgccgac cggcagaaga aacggaaaac gagaatccgg gagagccgaa
     3061 atacgcctac gcgccgccgg tacggctgcg ccatttcggc tacggaaagg gacaaagcgg
     3121 cgaggagccg cagctccttc cgctggaaga tgcagacgat ggggaagagg cggacgctgc
     3181 gcagccgggc gcgacggtgt accatccccg acaggtctcg tggaacgagc ctgtatcgga
     3241 aaaggaaaaa cacaagggct accgcgttga agcggaggaa gaaaaggaac tttccgaccg
     3301 ggcacggcgg aagaagctcc gcaaaggagt cgtcatcggc tgcgccgtgc tggttctggc
     3361 cggggcagga tatgcctttc ggcagcagct gtcagagtgg atcggcagcc tgaccggcca
     3421 tacgccggag gtcagcgccc ccgcagcggc agtggctacg cccgcgccgg tacgcagcta
     3481 tgacgcggct gtgccggtca gcgtcaccag ccgcacaagg gaggccattg aggcgctgtg
     3541 cggcggactg gaactggaaa acgtggtggt tcgggatacc agtatcctgt cccgctgcgc
     3601 ccgtcaggac ggccggtacg attattatct tttcagcgcg gcggacggaa ggcttctgtg
     3661 ctattttgag aatctgggcc cggatgacac gttccccatg gcggggggcg gctactatgt
     3721 gcgtcagtcc ccgtggctga ttcaggacga cggttccgcc atgctgcgac tgactgatgt
     3781 ggaggccgcg ctgggcgaaa aggtaacgcc tcatcccatg atgaacgggt gggcgctgct
     3841 ggaagcggaa agcggcgacg agtggaattt catcaacgcg gacggaacgc tgctcagccc
     3901 cctgtggctg gaaaaagcat ggcccatgac cggcgactat acgctggggc tggtggacac
     3961 cggcaatctg aacgacacgg agacccgcta cacgctctac attctgggca gcgatggcag
     4021 cagcgtgcgt tggcgcagcg aagctacacc cgacggcgtg gtgatcagcg cctgcggtct
     4081 ggcctatctg gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnngccagc ggcagtatga ttttctgtat gatgcaatcg cccctgcgga aagcgatctg
     4261 cagtggcgca cggacgctta tcagggcacg ggaggaaccg ccgctctgcg ctacgtcagc
     4321 ggcgctgctt acccccagcc gctttctcac tattttcggc tggaaaagga cggggaggaa
     4381 cagtatgtgg ccctgtccac cgcatccgcc tgcccggtgg agctgaactg aaatacggag
     4441 gaacccaatc atgaaacgaa ttttggccct tgggatgacc ctctgc
//
LOCUS       DAWAEA010000076         4480 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3023_length_4480_cov_2.429153, whole genome shotgun sequence.
ACCESSION   DAWAEA010000076 DAWAEA010000000
VERSION     DAWAEA010000076.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3023_length_4480_cov_2.429153"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2783..2792
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          873 a         1390 c         1332 g          875 t
ORIGIN      
        1 agcgccgccg aaagcctgcg tgaacatctg gcccatgccc atgcccgcgc ccatgcccac
       61 gcccataccg gccatgccat tggggttgtt ggcggcgtcc cgcatggcct cggcggcctg
      121 gaactgggcg tacttgccca tatcgcccag cacgcccatg gaggtgcgct tatccatggc
      181 cttttccact tcttcgggca ggctcacgtt ttcaatgaca aagttggtca gggtcagacc
      241 cagcgcttcc accttggtat tcagcgcgtt catgcagtac tggctcaggc cgctgtagtt
      301 ggcggcgata tccagcgcgg ggatcttact ctgggcaatg gcgtcgctca ggccgctgac
      361 caccatgctc ttcagctggc cctccacgcc ttccacggtg aacaggctgc tggtgccgaa
      421 gcactccttc atgaaggttt cgggttcctt cacgcggaag gcgaaggagc cgaaggcccg
      481 gatgcggatc atgccgaaat cggcgtcccg catcatgacc gggttggcgg tgccccactt
      541 ccggtcgagg aactgcttgg tgttgatgaa gtacacatcg ctcttgaagg gagaattgaa
      601 gccgaacttc cacgccttga gcttggtcag gatgggcatg ttctgagtgc tcaactcata
      661 acggccgggc tggaacacgt cggccagctt gccctcgttg acgaagatcg ccgcctggct
      721 ttcccgcacg gtgagctgcg cgcccatcat gatctccttg ccgttcatgt catatttgta
      781 caccatggta ttctggctgt cgtccgtcca ctcgatcacg tcgatcagct ggctggtcac
      841 caaatcctta aaaatcccca tattgttctc cttactgccg ccgcaggcgg acggcttttt
      901 tatttcagat tttcagcggc gaaggtcgac aggcccggca ccagccgcac ctcggcaacc
      961 tgcatatgca gccccagata gtggtcgatc tgctgggcgg tctcctccac gtaggcgcgc
     1021 accgcgcttt ccagcagggt gccgtccccg gtgacggggt agcagagcac ccgcacctct
     1081 tccggggaga tcacagccac gtgcaggtac atatcccgct gccgcgcgcc gaaaaagttg
     1141 cgcagggcgg tttccagacg ctcgctgccg taatgccagc gactggtcag gaaggggtcg
     1201 tccatatcca tggtcaggcg ggccagcacg cagggcacgt tgggcttttc cctgcatcgc
     1261 agcatgaaca gacgggcccg cagctccttc tccgtgggga tcagtccgca gacgatgccc
     1321 ttcagcagcc gttcctgact gtgggaaaga gagcccatca gatcgtattc gtcgttataa
     1381 tgatcggcgt gaaggtttcc cagcatccga tccagcgccc ggatgacctt ccattcctcg
     1441 tccggggtct tttcaatggg aaaacggagc attgccgggc actgttcttc cacaaagcgg
     1501 ttcagctcgt caaaagccgg gccgtcgtcc acggcgatcg cgtcgatctg gtgcttctgc
     1561 atacattcca gcgcttcctg cgtggtctga cgcagacgcg gcggcttgaa gcccaagctc
     1621 tcccagcctt ccatcgcggt gaacaagtcc ctcacactct ggtttccggt gacgatcagc
     1681 agcctataca cgcgtttccc tccatattgg tcaaaatgac acgaatttat tataccctgt
     1741 caccgggaaa atttcaatcc ttcccggatt ttggctgaaa aaaggcggtt ttgagcaaat
     1801 gccgccgatt ggttcccggc cttttcaggt gtataataaa ggggaagcgc gctcattggg
     1861 ctgcgcggaa gggagcataa ggatgaaaac aggactggtt ctggaaggcg gcgggatgcg
     1921 cggcatgttc accgccgggg tgttggacgc cttttacgcc cggggccttc attttgacgg
     1981 ggtgatcggc gtttccgccg gggcggtctt cggggtgaat tttctatccc ggcagccggg
     2041 acgcgtcatc cgctacaaca agcgctatat ccgcgacccc cgctacatgg gccccggccc
     2101 gcttttaagg accggcaatc tgttcagccc ggactttgcc tacggcacgg tgcccacgga
     2161 gctggatccc tttgatcagc agagctttca ggcctcgggg gtaccctttt acgccgtggt
     2221 gaccaacatc gagaccggcc atgcggaata tcccctgatc gaaaacgtgt ttgagcagat
     2281 ggacacgctg cgggcctctg cgtcgctgcc ctttgtttcc aagcccgtgg cggttgggga
     2341 cggattgtat ctggacggcg gcatcgtgga cagcatcccc ttcgaggcca tgcggacgct
     2401 gggctacgac cggctggtag tcgtcctgac ccgggacggt tcctaccgca aggggcccat
     2461 gccctccctg cctatcgacc tgtggtacgg aaaatatcct gccctgcagc agcggctccg
     2521 ctgccgtcac gaggcctaca atcaaagtct cgaccgtctg cgccagctgg agcaggacgg
     2581 cgtcgccaag gtcatccgtc cccgccgtcc catcgaaatg ggccggttgg aacgagaccc
     2641 ggcggtgctg cagcgggtgt acgatcaagg atatgccgtc ggccagaaaa cagagctgta
     2701 aagaaaattc cccggttccg aagcaaattg ggcgctgcgg aatcgtggga actttttact
     2761 tcacatgaaa atacttatgc agnnnnnnnn nnggacaggg ccgccagtac gatcagcccc
     2821 cactggcttt ccacgccggg caccagcagg catacgcccg ccgacaggaa cgccaccaga
     2881 cacaacagca tcagtggaat gatgttctgc ttctgccggc tgaaatcggt gaaaaacagc
     2941 acggcggcca cggtcagccc ggcaaagcac acgatgggca ggaggttccg aatgcggccc
     3001 ggggcaagga tcaggtcgat cagcaaaaga aggccgcagc actgggccag taccttggtg
     3061 aactggctga cccggttgta ttccaccatt gtcggggcta ccagatcgcc ccagagcatc
     3121 cacagccccc acagggccac cagactccac gcgctgccgc ccaggcacag gttcaccacc
     3181 gggcaggcca ccgccgccgc cagaaagggc cagcgggccg cgcagatcag ctgatgccgc
     3241 tggatgcggc gcttgggggg gatgggatag acgcactcaa ccttcataca gaacgcttcc
     3301 tttcacggca tagggcacct gatcgccgtc cagcaggcgg cacagtgctt cttccagcga
     3361 ggggtcggcg gtctgcttgg cgatggtcag cacggccttg ccgccgcagg tcaccagcgc
     3421 acagcccgca cgattcgtca ccagcgtgcc cagcaaaaat tccagatgat ccacccggct
     3481 ctgcatctcc gggggcaggt tcaccacacc cagattggac agggtgttgg tgaaaatttt
     3541 atcccccaga aagccgtaga tcacccgggc gatgggggcc ttgataaaca gcggcacaaa
     3601 gcgcaccgag ttcaccagcc gggtggagga atggatcatc tcggtcatgg cctcccggga
     3661 ggctttctcc gtcagctgat gctgaatttc cggcagaatg tcgtccagtg tggtcacctg
     3721 ctccatatcc agtcgaatgc cgcagtacat ggaaaaattg cgcagcgtgg gcgagggata
     3781 gaacttgcgc atattgacgg gcacctgaat gttgaagctg ccgcccatgg cctccgtggc
     3841 ccgccgtccc gccaaaaaca gaagccccag cagataggcc gtgacggtgg cgttccggtt
     3901 gcgggcagcc tcccgcagct tttccgcgtc catttcgaag tgcaggatgc gacaggggtg
     3961 caccggggcc agccggccgc tgagctgcag ggccgcccgg tcggtcaggc cggagccctt
     4021 gccccggcga tctgccgccg ggaaagcgtt ttcgttttcc ccggccagcg gcggccgagt
     4081 cacgtccaga atgccgtcgc cgcaggggca gtccgtgccc agcagccgca gatattccgc
     4141 cagcagggtc ttgaggaagc acatgccccc ggtagcgtcg gtcagaatgt ggaaaaactc
     4201 cgcgctgata cggttttcat aatacagcac ccggaagctc tgagagccgc tggcggcgat
     4261 cttcagcggt cggacgggaa tatccgtctc cggctggatg cagtagcgac gtttggtctg
     4321 atccagataa tgccagaaaa agcccttgcg cacggtggtg gcaaagccgg gaaaacgctg
     4381 gatggtgaaa tgcagggcga tctgcagaag ctccggcttc accggctcct tcagaaccgc
     4441 cgccagccgg aacacagaca tctgcccgtg cttcatggac
//
LOCUS       DAWAEA010000077         4480 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3024_length_4480_cov_2.255819, whole genome shotgun sequence.
ACCESSION   DAWAEA010000077 DAWAEA010000000
VERSION     DAWAEA010000077.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3024_length_4480_cov_2.255819"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    312..321
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          806 a         1529 c         1236 g          899 t
ORIGIN      
        1 cagcagctcc acatgctctt tgatcttttc cgccgtgaag cgcatttcct cttccgtatt
       61 ctccgggcaa aggctgatgc ggatggcccc gtccatcacg gagttcgggg ttttcatggc
      121 ggtcagcacc gccgaatgcc tgcctttttt ggacgagcag gccgagccgg tgcccacgta
      181 aatgccgtcg tcctccagcg catgcagcag cgtctccgcc cgaaccggcg ggaatgccac
      241 actcagcaca tgccctgcgc tgcggtcatc ttccgcccgg aagcccacca cggtggtctc
      301 cggcagcttt tnnnnnnnnn nccgcagacc ggcgatgccg caggtgtttt ccgtgccgct
      361 gcgcaggtcg tcctgctgtc cgccgcccac gatctggggc cggaaacgga agccatcctg
      421 ccagaccagc gcgcccacac ccttagggcc gtgaatctta tgtccgctca aagcatagga
      481 ctgcacgccc agctttttca gcgagaaggg cagacgcatg aagccctgca ccccgtccac
      541 gtgaaggacg gccttggggg ccagccggtc gcgcagcgcg gcgatctccg caatgggcat
      601 gatcacgccc gtctcgttgc agacctgcat cacgcagatc atggccgtgt tttccgaaag
      661 cattccttcc agcgcttcca gatccaccga gccttcccgg gtcaaaggaa tctcgacggc
      721 cttgcagccg aaacgggctt ccgcctcccg gcaggcgttc tttaccgccg catgttcagc
      781 ggcggtgtag agcacctcgc ccttgccccg cagggtttgc aggtggccca gaatagccag
      841 attatcgctt tccgttcccc cggaagtgaa gatcacattt ttagccggaa cgcccaattg
      901 atccgccagt gccttccggg cggcggtcaa ctccttttcc gcctgcatgg cggggccgta
      961 cagggcggac gggttgaaat agacctcttc catggaatga accatcgcgt cccgcaccgc
     1021 ctgactgggc tgtgtggtcg cgctgttatc cagataaacc atgtcgttcg cgcctctttt
     1081 tcagaagatc aggcccgcca ggcggtaggc ggcagatact tgcggatcat gcctaccagc
     1141 tcctcggagg gctccagaac gatgatggtg cggttgtttt ccttctgata gtagaagtag
     1201 tacagcttgg ctccccggtt caggaaccag tttttccgat tgatgccggg catgttgatg
     1261 agcttgcgga actcgttgga atccacggga ccgaaggctt ccacattctt cacccgcatg
     1321 gtgcccagcg ccttgcgctt ctgattgttg aacacctgag caaagtccag atcgccgttg
     1381 gtgaaagtgt actcgtattc cgttttcagc cgatccttaa acaggaacag cagcaccgcg
     1441 ccgccgatga agatcaccgt ctcgatcagc gcgggcacac tgaactgata cagcagggtc
     1501 tgcagcatca gcagcccgaa caggccgaaa atcaccagca tgatccagga cagcacgtag
     1561 aggatgtcat caaaggtacg attgtgctta accacgactt cttccataaa gtgatccata
     1621 gtattccctc ctgtgtaatg ccttttaagc aaataaaatc atggccacca ccacgctcag
     1681 ggcaaacagg ccgctgtcaa agccatacag gccgccgatc atgaccgccg tgcccgtcac
     1741 catagccgaa tgggagctgg gcatgccgcc cgacccgtga aagcgggaaa aatcccacac
     1801 atgctccaca tgatgataaa tcggaatctt caatgcctgc gccacgaacc agctaatcag
     1861 cgcggcgaac agcacatgat tggctcccaa gcctaaaatc gagtgcatag gcgctcctcc
     1921 ttccgcctgt cagacgctgc gggtcaaagt ctgacgggcc agttcggtca ggaatttccc
     1981 gcgttctccg aaggactcca tggcggcggc agcatcctcg acagcctgtc tcgcgtcctg
     2041 gtgagcctgt tccacgcccg ccacagcggg ccaggtcatc ttgccccgct gggcgtccat
     2101 gcccacatcc ttgcccagca gcttgacgtc tccttccaga tccagcaggt catcctcgat
     2161 ctggaatgcc agccccagtc cccggccata ccggcggccc aattccagtt gggcttcgtc
     2221 cgccccggcc agcatcagac cggcggtcag cggcgcggtc agcaggtcgg cagtcttgtg
     2281 caggtggatg tagcggacga tgtcctttcg gggcggctgc ccctccatgg tcacgtccaa
     2341 tacctgaccg gccaccatgc cccgaacgcc cgcccgttcc gcgatctccc ggatggcagg
     2401 cagcgcccgt cccggtgctt tctgcgtcag cggcgcggcc agcatggtct cataagccag
     2461 gttcagcaga ccgtccccgg ccagaatggc catggcttcg ccatacaccc gatgattggt
     2521 gggctttccc cggcgaaggg cgtcgtcgtc catggcgggc agatcgtcgt ggatcaagga
     2581 ataggtgtgg atcatttcca gcgcacaggc gaagggcacg gcctcctgcc agtcgtcccg
     2641 cagcaggtga tacgcagcca gcagcagcac cgggcggatg cgtttgcccg gcaacagcag
     2701 actgtagcgc atggcgctgc gcaggggctg gggcacctcg ccctcggcgg gggcttcctg
     2761 cgccggttcc gtcaccggcc ggcccggatc gggcagcagc agaccgtagt tctccagggc
     2821 cgcctgaacc gcgtcaaagt aggcgttcat gctctgccgc tcccttccag ctcgctcatg
     2881 ctgatctgcc cctccatagg ctgatcggcc gcttcgtcgg ctgcgcttac gcgaatctcc
     2941 ttcagcgtgg cctgcatccc ttccagccgt tttttcagtt cggcggtcag agccacaccc
     3001 tgctcataaa gcttcatcag cgcctccaga ggcagctccg actgctccat ctgggtggcc
     3061 atcacttcca gcttttgtag ttcttcctca aaagaagccg ttttcttgac cgttctcgcg
     3121 ggcatattct tcccgtcctt tccatgggtt caacgctcgg tttccaccgt ctctacccgg
     3181 gtcagcagac tgccgtcctg cagcatcagc cgcacagcat cgcccgccgc cgcgtcggcg
     3241 gtacttttca gcagacgacc gtccttcaac gccacggcgt agccccggcg cagggtctcc
     3301 tgcggcccgg aggaacgaag ccgtgtctcc gcatgacgga gccgcgcttc catctgctgc
     3361 aatcgctggg atgcggcgct gtccagcctt tgccggacgt tttccagccg taggccatac
     3421 cggctcaaaa gctgttccgg ctcccgagcc ttcaagcggg tctccgcctg tttcagccgc
     3481 gcttccgcat cgctgaaagc cagcgtcagg cagcgttcca gccgctccgc ctgctggtcg
     3541 atctcgtcca gcagagcctc cctcggctgc acgcacagtt ccgccgccgc cgagggcgta
     3601 gcggcccgca catccgccgc aaaatccgca atgggaaaat ccgtttcatg gcctacggcg
     3661 ctgaccaccg gcgttcgaca cgctgcgatc gcccgggcca cgatctcctc gttgaagggc
     3721 cataagtcct ccatgctgcc gccgccccgg cccacgatca gcacctctgc ctccggcatc
     3781 cgatccagcc ggcgaatggc cgcggcgatc tccctggccg ccccctcgcc ctgcaccttc
     3841 accgggcaga gatacagcgg catggccggg tttctgcgcc aagtgacggt acagatatcg
     3901 tgcaccaccg cgcccgtgga actggtgacg atgccaacgc cccggggcag caggggcagg
     3961 ggctttttca tcgccgggtc gaacagcccc tcggccagca gcttctgttt cagccgctcg
     4021 aagcgcagat acagctcgcc caacccttcc tgctccatcc gttcgccgta aaactggtag
     4081 gagccggtct tcacatacag gctcacactg cccgtcagcc gcacgctcat gccgttttcc
     4141 ggctgaaagc gcaccgtctg agccgcctga cggaacatga cgcagttgat ggccgcttct
     4201 tcgtccttca gagtgaaata ccagtgcccc gacacatgac gcttgaaatt gctgatctcg
     4261 cccgtgatgc agatattttt cagcatcacg tccgacgcca gcgtccggcg gacgtattcg
     4321 ttcagctgag cgacgctcag ggtcatccgt tcgttggtca tagcgacgcc ttctccgccg
     4381 cagacaccgt gttttccatc agcatggcga tggtcatttt gcccacgccg ccgggcacgg
     4441 gccagatcca tccggctttt tccttcacgt gctcaaaatc
//
LOCUS       DAWAEA010000078         4407 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3096_length_4407_cov_3.349494, whole genome shotgun sequence.
ACCESSION   DAWAEA010000078 DAWAEA010000000
VERSION     DAWAEA010000078.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3096_length_4407_cov_3.349494"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3441..3450
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4104..4113
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          827 a         1338 c         1421 g          801 t
ORIGIN      
        1 agtatgtgct gctgatgatt gtgaatgtgt tctatatctt cgtaggcatg atcatggata
       61 ccggtgcggc catcatcctg ttcgcgccca tcatcgggcc cgtgctgacc atggtgggtg
      121 tgaacccggt acatctggcc atggtcacca ttatgaacct gaccatcggc ctgctgacgc
      181 ctcccgtggg cctggtactg ttcaccgccg tcaacgtggg aaagaggccc ttcggcgccg
      241 tggtcaaggc cttctggcca tttatcatca tatcctatct ggcgctgttc gttgtcacct
      301 ttattcccca gatcgtcacc tggctgcccg gcgcactggg cttccagatc gccctttcat
      361 aagcaatgaa ctttgcgggc gggcagaaag cgatgaaatg ttggcccgct atcaggctgc
      421 ggcctgatgc cgcggcgcgg aaaaaatcgt tttctgcccg ccccgaagta tgttctgaca
      481 ggagagataa ccatgcgcat cggtgttttt tacgcgtact tcccctatga ggacagcgtg
      541 gactggcttg ggaccctgcg ccgggccaag gcctgcggcg cggaccatgt ggagctttcc
      601 tccatgcggc tgctccggca ggaccggccc ctccgccgcg agatcgtgga gctggcccat
      661 gagctggacc tgacctatac cttctgcacc tcccttcccc cggacgggga tgtggcctcg
      721 gaggatcccc gccagcggca gcgcggcgtg gacgccatgc gccgcaacat cgagctggca
      781 gcggaaatgg agggcgccgt catcggcggc atgctgcacg gctccaccgt gaagcccggg
      841 gtgccggtgg ataacgccac ccgccagaga agactggagc acagcgccgc cgccgtgcag
      901 cagatggcac agaccgcccg ggcctgcggc gtgcgcatgg gcctggaaat gtgcaaccgg
      961 tatgaaaaca gcatgctcaa caccgtgtcc caggggcttg ccttcctgga catggtggca
     1021 agcccggctg tggggctgca tctggacaca taccacatga atatcgaaga ggatgatctg
     1081 gccgccgcca tcatacaggc gggggaccgg atcgtgaacg tccatgcctg cgacaacaac
     1141 cgcaagctgc ccggcatggg ccatgtcaac tggagggaga tcctgctggc ccttcacgcg
     1201 gcgggataca ccggcgctct ggccacggaa tgcttctccc atccctttgg cgtgtggaca
     1261 aatgaccacc gcatctggcg cagttatgtg aaagacggca ttgaccagga cctgaagcgg
     1321 tccatggcct atctgcacgc gctggagcgg gacctgtaat actgattctt gattaaaaaa
     1381 tttaatcaag aatcccgtgc gctgtgcgca cgcacagcgt gtgaaaacac gctgtgacgt
     1441 ctcatacaaa acctgacgca ccttcggtgc tataaaaagc ttttcaagct ttttaacagg
     1501 ttttagtata aaaacgagta caaaacggcc tgcgggccgt ggttgataga gcaaattcga
     1561 ggtgtattgg aatgaacctg tttgatgtat cgggcaagaa agtgattttt accggagcca
     1621 ccggcggcct gggccagagc atggcggagg gcctgctgga ggcgggagcc gaggtggtgc
     1681 tggtgggcgc atccgggcgg tgccggacgc tggcggagga ataccgggcc agaggctgga
     1741 aggcccacgg cctgccctgc gacctggcca gcacggagga gatccgggac tgcttcggca
     1801 aggcccttga cctgctgggc ggtgagatcg acgtgctggt aaacgcggcg gggatccagc
     1861 ggcgcgcccc cagcgaggac tttccgctgg aggactggca ggcggtgata gacgtgaacc
     1921 tgaccgcgcc gttcctgctg agccgcctgg ccggcaagca gatgatccgc cagggccggg
     1981 gcggcaggat catcaacatc gcctccatgt gcagctattt cggcggcagc accatcccgg
     2041 cctattccgc gtccaagggc ggcatcgccc agctgaccaa ggccatgtgc aacgactggg
     2101 caaaatacgg catcaccgtc aacgccatcg cccccggtta tatggacacg cccatgaacg
     2161 ccgccctgac ggatccccgg aacccccggt tcaaggagat cagcgaccgc attcccgccg
     2221 gacgctgggg caccggcgag gacatgaagg ggctgaccat ctttctggcc tccggcgctt
     2281 cggactatat taacggcgcc gtcatcccgt gcgacggcgg ctatcttgtg aaataagtga
     2341 ggaggcagtt gatatgagca gatatctggt ctgcgtgtcg gacaagcggc acgcatcgta
     2401 tgacatcgaa agggacatgc tggcgggcat cggcgcggag ctgcggctgt gcagctgcgg
     2461 caccgaggag gatctcatcg cccagtgcgg ccaggcggac gccgttctgc tggatctggc
     2521 ccccatgacg gccagggcca ttgcgggcct ggagaagtgc cgggtcatca gccggtacgg
     2581 cgtgggatac gaaaacgtgg acctggacgc cgcgtccaag gcgggcatcc aggtgaccaa
     2641 tgtgccggat tactgcatgg aggacgtttc cgaccatgcg ctggcgctga tgctgtcctg
     2701 cctgcgccac attcccatgc gggaccgcat ggtgcgccag ggggcgtgga atctgcaggc
     2761 caccagcttc cgcctgaagg gccggaccct gggcgtgatc ggcgccgggc gcatcgccag
     2821 ggccctgatc cgcaaggtca gcgggttcgg tctggcggag gtgctggcct atgaccccta
     2881 cctcagtgcg gaggagctga gccggatcgg cgtgcgcaag gtggaactgg cggagcttct
     2941 ggcaaaatcc gacatcatct ccctgcatct gcacgccaac gcggaaacct gcggcatgat
     3001 ctgccgggag acgctggagc agatgaagga aaccgccatt ctcatcaacg tcagccgcgg
     3061 gccgctggtc aatgacgacg acctgctgga ggcgctgcgc agtcaccgaa tcctggcggc
     3121 cgggctggat acccacaacc acgagccgct gggcgcggac tcccccttct gccggctgga
     3181 caacgtggtg ctgacggacc acaccgccta ttccaccatg gaaggcgtgg aggagctgaa
     3241 aaccaaggcg gcccagaacg tgatcgacgt gctcaccgga cggccagtcc gttacccggt
     3301 gaatcatctc taagcgatac tatttatatt tagaaaggca agtgatcctt tgtgaacagt
     3361 ttggaaaggc tccggaacgc ggcggtggtt cccgttgtgg tccttgaaaa cgcggatgac
     3421 gcggtcccca cggcgcaggc nnnnnnnnnn ccttccgcac cgacgccgcg gaggattcca
     3481 tcgccgccgt gtcccgtcag tgcccggata tgctggtggg cgccggtacg gtgatcaccc
     3541 tggagcagtg caaaaaggcg gtggagatgg gcgcgggctt catcgtgtcc cccggcttca
     3601 gcgaggaggt cgtggactgg tgcgtgcggc acgatgtggc cgtgaccccc ggctgcgtga
     3661 cccccacgga gatcatgtcc gccatgtccc acggcctgca ggtggtcaag ttcttcccgg
     3721 ccaacgtcta tggcggcctg gacgccatga aggccctgtc cggtcccttc ggcggcatcc
     3781 ggttcatccc caccggcggc gtgaacgaaa agaacctgag cgattatctg gcggcgccgt
     3841 ttatccacgc tgtgggcggc agctggctgt gcagcaaggc ggacatcgcg gcgcatcgct
     3901 ttgaccgcat cacggagctg tgccgccggg cccgcgccgc ggcgctgggc ttcgagctgg
     3961 tccacgtggg cgtcaacgcc cccgacgcgg acgcctccat ggaactctgc cgcatgctgg
     4021 acgcggcctt cggcctgggg gtcagacccg gcaattcctc caatttcgcg ggaagcgccg
     4081 tggaggtcat gaaggccccg tatnnnnnnn nnngggtgtg gacgccaaca ccggctcgct
     4141 gggccagggc gtttccattg ccggcggcat ggcgcttgcc gcaaagtata agggcgagaa
     4201 gctgacggcc gtttacctgc ggcaggattt cggcggcttc gccatccatc tggttcagaa
     4261 ataaggaggt ctgcaccatg aaattcatga cattcggcga gatcatgctc cgcctgaaag
     4321 caccgggaat gacgcggctg ctgaccgatc actatctgga ggccaccttc ggcggcggcg
     4381 aggccaacgt ggccgtctcc cttgcca
//
LOCUS       DAWAEA010000079         4346 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3152_length_4346_cov_1.961547, whole genome shotgun sequence.
ACCESSION   DAWAEA010000079 DAWAEA010000000
VERSION     DAWAEA010000079.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4346)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3152_length_4346_cov_1.961547"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3147..3261
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3960..3969
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          877 a         1274 c         1229 g          841 t
ORIGIN      
        1 ctatgccgtg gaaatctggc agcggctgaa ggggctggcc ggacggcggg ttattgcagg
       61 gctgttcctg ttttgctgct tcttcggttc cgcgctgacc gtggcccggg aatggatcag
      121 cgactatcag gcctacagcc cctcggatat tcaggctgcg gagtacatcc gggaaaacac
      181 gccggagcat tcggtatggc tcaccggcaa cgagcatctg aacccggtca gctcgctggc
      241 gggacggacg atcctgtgtt ccagcgaatc gtacctgtat tatcacggct tcgacacctg
      301 gccccggcgg ctggagatcg ccgccttctt tgagaacccg gcggacaatc tgtcgctttt
      361 gaagaagtat caggtggaat atctctacgt cagcgcctac gagcgcagcg ccagctggtt
      421 cgacctgaac gaaagcgcgc tggaagcgct gttccccgtg atctacgagg atgagggcgg
      481 ccagcaccgt attttccaag tgccggagga ataccgccaa tgaaccgatt tttgcgctat
      541 tcgcaggaaa aagggcggaa gatccgcatg atgctgatgc tgaacggcca gcttgcgcag
      601 aagacggcgc ttgtgttaag ctatgacgag caggccgccg tgctgcagct cggccagcgg
      661 aagcagcccg tcaccgtgcc cctttccgat attctcggct gcgactacgc ccggggcgat
      721 tcgggcgagg aatgacgcgg cctctcgccg ccttcctcaa aaaagacaga aaagagggcg
      781 aaacaatgtt ccataaaatc aaagattatc tggaaaaaca tcccaagcag tacgagttgc
      841 tgcggtacgt cgtcgccggg gggctgacca cggtgctgag catggtcatt tcctatggga
      901 tggagttcct gctggccgcc cgacccgaac ggacggcggg ctttttgcag tgggtgatcg
      961 actgcatcaa cgccgccaca gctcagcagg tgatgatcgc caacgccgtc agctgggtga
     1021 tcgccgtgct gttcgccttc tggatcaacc ggaagatggt gtttcaggtg aagggcggca
     1081 ccggcgcttc cgtgctgcgg gagctgggag agttcgcgct gggccgggtg ctgagcttcg
     1141 ccctgttcga ggaaggcatg gcctatctgc tcaagctgat cggcgtgagc aatgtggtca
     1201 accgcatcat tgtgctcgtc gtggtgatga tcttcaatta tgtggtcagc aagttctggg
     1261 tattcaaaga aaaaaaggaa aaagcctgac cagcatccgg cagacgccat acgccgccgc
     1321 cccgtgaagc agcctgagca gaagcagctt cccggggcgg atgcttttgc ccagaaacag
     1381 caggttctgc atcagaatgc tcagcccgcc gaagctgcat agggcgcaca gcaggggcag
     1441 atcgggcgta ggggaaacgt ccagcagggc atgggctccg ccgccgatct ccaaaacggc
     1501 ccataatatc gcaggaaatt ccgggtgcgc cgccgcccag cccggcagca gcgccgaaag
     1561 tccctgccga aacagcgccg ccaaaatgga aaacagcatc atggcgccgc atactgacag
     1621 cagcgccggg ccggtctggc gcaggctgtc gggcagcgcc tccagcaggg acgcgcgccc
     1681 cgccgtccgt tcctccgggc ggacgggctc cgccttctcc atggaggaaa aacggcggga
     1741 cagcctgtgg atcagcccgg ccgcagccag cgcccccagc cagtgggcgg tcagcatcag
     1801 ccagcctccg gccgggtttg gcaggcggcc gcccagcgag cctaccaaaa acaaagggct
     1861 catgaccccc gtcgccgccg ccatgggcag cagctcccgc ccggcaagcc cgccccgctc
     1921 ccagagccct tccagccgct gggccgacgc aggcgacccg gacagccagc agaagcacac
     1981 cgtcatccac cgctgctccc gcccgcccag ccgggcgcac aggccgttta gcaccatcat
     2041 gggaaacagc gcgggcatca cgctgcgcac aaacagcccc gccgcgccgt tgaccgcgtc
     2101 cagcaccgta cccgaatcca gcagcatcag cgccgccagc ccaaaaagcc acgccatgaa
     2161 acttcctcct ttgccaagcg taccgccgct tcgtccctgc ccgcggcgtt ttttctgaaa
     2221 ttgccggatc tcgctccgtt ttttccgccg aagaccgtct tttttcaggc cgcaaaacag
     2281 cttatgcacc ttgattttcc gtcatttccg gcccgccggg agaatttcgt ttcaaaaagt
     2341 tcacagttta tgcaacaaaa cgtcacgttg gtacgtcata ctatatgtga ggtgatcgca
     2401 tggcaacgat tttgattttt tccgaaaact ggcagatcgc gcataagctc tgcgattcct
     2461 atgcaaagga aaaatacgac gcaagattat gcagcgatcc tgaccagatt tccgccgccg
     2521 cggatgaaaa cggcgcgccc ctgatggtga tcgacgcggc aatgaaatgg gtggtctgcc
     2581 gtccgatatt cgagcggtgc cgccagacgg gaagccccat tctgttcgtc accagcgacc
     2641 ggcgcatgag cgaccatctg ctggccctgt atccgggccg gtcggacgtg gtggtgacgc
     2701 ccttcagccg caagacgctg gcggaaaaga cccgcattct gctgggcggc gaaacgcccg
     2761 cccgggagat ctccgtggac gaaacagcgc aggtggccct gctcaacggc cgccgggtcg
     2821 agctgaccgc ccaggaaatg gcccttctgc tggccctgat gaatcagccc gacacccccg
     2881 tcagccggga acagctgctg cgggaggctt ggggatacca gagcatgggc gagacccgca
     2941 ctgtggacgt ccatgtacag cgtctgcgca aaaagctggg cgaggactgg atcgagacgg
     3001 tctacaaatg cggctaccgg ctgcggctgg cctgagggaa aaaacaaaaa gcaatccccg
     3061 gcaggacagc gcgatctgtc ctgccggggg taattgattg gaaaaaacgc agtagaacga
     3121 agaaaaacgt atcttccgga tcaaagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn ncgtgctttt cctgcgggaa gggctttctc ggtatgtccg
     3301 gtgttcacat ttttatgacc ttttcagcga acggttcttc cgaccgttcc tttacgcgct
     3361 tcgctccatg gagagtttgt ctgcgatcat ggcgatgaac tcgccgttgg taggcttgtc
     3421 ctccggggtg cataccctgt atccgaaggc agtgttcaga gtctcgaccc ggccccggtt
     3481 ccatgctacc tcaatggcgt ggcggatggc ccgctccacc ttgctggcgg atgtgccgaa
     3541 ataacgggca atgccgggat acagctcctt cgtgatccga ttaatcacat cgggatcggc
     3601 caccaccatt ttcacggcct gacgcaggaa ctgatagcct ttgatatggg ccggaatccc
     3661 catggtcaaa aacagactgc ccagccgctc gtccacattt tgacggcagg gactttttcg
     3721 gagatcggag ggcaaatttt ccgccggagc agtctttctg cccgtcaggc tgcggatgtg
     3781 gcacatcaga taacgcggct caaagggctt gaccatataa aaggcggccc ccagctcgat
     3841 cgcccgggca acaaaatcat cccggcccag cgcggacgct acgatcacgc cgggctggta
     3901 gggcagctta cgaagctcct ccagcagggt gtagccatcc attcgaggcg tgatcacatn
     3961 nnnnnnnnng gtttcctctt tcaacagccg cagcgcatca aagccgtttc cggcttcgcg
     4021 caccagcgcg atttcctcct gcctgcgcag aaattctgcc atagcgctgc ggttctgaga
     4081 ctgagcatca acgatcaaga tccgggtttg ttccatggtg attgcctcca ttactgaatg
     4141 atattcctcc agccgaaatc agcgcggttt ttctttcggc attggttcct ttttcgttct
     4201 tttttgataa gcgctgtgtt ttttctgttt ctccattgcc ggattttttg actggcggtc
     4261 cactttgtgc taaaatacag gagaaatttc cctttttcgg gaactgacgt tttggatata
     4321 gcacgttcga atgcaaaatt caatcc
//
LOCUS       DAWAEA010000080         4310 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3183_length_4310_cov_1.841128, whole genome shotgun sequence.
ACCESSION   DAWAEA010000080 DAWAEA010000000
VERSION     DAWAEA010000080.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3183_length_4310_cov_1.841128"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    486..495
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2091..2100
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          889 a         1384 c         1151 g          866 t
ORIGIN      
        1 catctggccc gcgtctgcct gcgtgtcata aagaacgctg ccgtcctccc ctacccaggt
       61 cagacggtag ctttccggct tcaggcggga aaggtagctt tctccttctt cctccacggc
      121 ggagaccgcc agactgagct cgtcccgcag ctggccctcc tgcaggtttt caaaatagcc
      181 gtacagacaa tccagaataa tcagaaagct ggccaacagc accacgcccg ccaccagcag
      241 gatggagcgg aaaattcgtt tattcatact ttggttcctc cagccggtag cccacgttgc
      301 gcacggtctg gatcatgccg ccgtaggggc ccagcttctg ccgcagggtg aggatgtggg
      361 tgtcgatggt gcgggtctcg ccgtaaaagt cctgccccca caccttgtcg aacagctgat
      421 cccgggtaaa ggcaataccg gggttctgca ggaacagccg cagcagctca aactccttgt
      481 aggtcnnnnn nnnnncagca ccgccttgac gcaggacacc agctccatca ccccgaaggg
      541 cttggtcagg taataatccg cgcccaagtc cagcccctgg attttgtcga tctccgaatc
      601 cttcgccgtg gccatgacca ccgggatatc ttccagattc gggtcttccc gcatacggcg
      661 caaaagctcc acgccgtcga ttccgggcag catcacgtcc agaatgacca gcgtgggctt
      721 ttcctgctgc aaggcctccc agaaggaggt tccgtcctca aagccgcggg cctccatgcc
      781 cgtggaacgc agggcgtata cctcgatatc ccgaatgctg gcgtcatcct ccacgcacca
      841 gatcatgttc agtcctcctt gtgggtcccc gtgacggaga agatcaccca ctgcgcaata
      901 ttcaccgcgt ggtcgccgat acgctccaga tacttggcga tcatcaacag atccagcgcg
      961 tactcgccgt ctccgggatc ctgcgcgatc ttttcgatca gccgtttctt cacctgatcg
     1021 aagtagtgat ccaccgtatc gtcatcccct acgacttttt ctgccaacag tatatcatgt
     1081 tttacgtaag catcaacact ttccgtcacc attttgatgg cggcggcggc catttcggcc
     1141 atgagctcat catcctccga gccgcgtcca ttcaggaaac agacgatctc tgcgatatcc
     1201 acctatgcgc tccatgtccg tgatcatttt cagggcggcg gagatctgcc gaaggtcccg
     1261 ggccacaggt tgctgctgca gcagcatttt caggcacatg gactcgatgt tgcgctcctt
     1321 ctggtcgatc tccgcatcca gcggggccac cttccgggcc tgttcggggt cgcccgccgt
     1381 cagcgcctgc gagaccatgg cgatcacttc ttcgcacagc gcgcccattt cgatcagttc
     1441 tttattcagc tccgccagct gttcgtcaaa acggcttctc atcagccaaa cctccccgta
     1501 atatagtcct cggtgcgctt gtcctccggg ttggagaaaa tcttttccgt gctgcctacc
     1561 tccaccagat cgcccagcag gaagaaggcc gttcggtcgg aaatacgcac tgcctgctgc
     1621 atattgtgta tttttccttc agctccatcg ccagctcctc gatcttggag gtggaaatgg
     1681 ggtccagcgc gctggtgggc tcgtccatca gcagcacttc cggctggacc gccagcgccc
     1741 gggcgataca gagccgctgc tgctggccgc cagaaagacc cagcgcgttc tttttcagcc
     1801 ggtcttttac ctcgtcccag atagcggcgt cccgcaggga acgctccacc acgtcgtcca
     1861 gccgggcgcg gtttttcata ccatgggtgc gggggccgta ggcaatgttg tcataaatgc
     1921 tcatggggaa cggattgggc ttctggaaca ccatgccgat ctccctccgc agctcgttca
     1981 cgtccatgga gggctcgaaa atatcctgct ccttgtagcg cacctgtccc tcgatcttca
     2041 cgccggggat caggtcgagg atggtcttgc tctccttgac ggtgacggtg nnnnnnnnnn
     2101 tttcagaaac gtggatttac cgcagccaga agggccgatc agcgcggtga tgctcttttc
     2161 gggaatgtcg atattgatat ttttcagcgc ctgatggttt ccgtaccaca ggcacagatc
     2221 cttgacagtg atgatattgc tcacgtggtt ttccttcctt tccgcttcat ggggaacagg
     2281 cttacagccg cttcttccgg cggtaatacg cgcccaccag atcagccgcc agattgatca
     2341 gcagggtcag caccatcagg atagcggcga tggcgaaggc cacgccgaac tcgccctgct
     2401 ccttggcgta gacgtacagt gccacagtca gggtcgcgcc cgcgctgcgc agtccctgaa
     2461 agaatccgtt cagtgcgtgg gcgaaaccgg ctgtgaacaa cagcgccgcc gactcgccca
     2521 gaatgcggcc cacggccaga atgcagccgg tgataacgcc gtccacgcag ccgggcagca
     2581 ccaccgtgcg gatgacccgc cacttgcccg ctcccagccc gaacgcgccc tcccggtagc
     2641 tctgaggcac ggttttcagg ctttcctgcg tggtgcgcat gatggtgggc aggttcatga
     2701 tgaccagcgt cagcgcgccc gccaacaggg aagtctgcat attcagaaac tgacagaaga
     2761 acagcattcc caccagaccg tatataatgg aaggaatacc cgaaagcgtc tcagcggcgt
     2821 actcgatcac cgccaccagc tttttattcc gggcgtattc cgtcaggtaa atggccgcgc
     2881 ccacgcccag cggcagcacg atcagcagcg tggcgatgac gatatagacc gtattgagaa
     2941 tatccggcag aatgccgatc tgattgcgca gatagctggg cttggtggac agcagctccc
     3001 acgtgatatt gggaatgccc ttccacagca cgtatccgat caggaacaat accagcgcgc
     3061 cggtcagcag gacggagaag agcatgccgc tgcagccccg acgcgtagga catttcactg
     3121 gccaccgccg tggtcaggaa gcggacgctc tggaacaggg cgggcatgtt gggcacattg
     3181 ccggacacca tcatcaccgc catagcctcg ccgatggccc gacccacgcc cagcaccacc
     3241 gccgccgcga tcccgctgcg ggccgctggg acggataccc ggaaataggt ctccaccggc
     3301 gtagcgccca gcgccagcga cgcgttctca tactccgagg gcacagcttc cagcgccgtg
     3361 acggacacct tgatgatgga cggcaggatc atgatcgcca aaacgatcat ggccgccagc
     3421 aggctggaac cgtccggcac atgaaacagc tggcgaatgc cgggcaccag caccagcata
     3481 cccaccagac cgtataccac cgacggaatg cccgccagca gactcacagc ggactgcgtc
     3541 accgccttca cccggagaga ggccaccttg gccagataca ccgccgcgaa aaagccgatg
     3601 ggcacgccca gcaggatggc cccggccgtt ccgtaaatgc tggtcataat aaagggcagg
     3661 ataccgtagg acggctccgc cgccgtagac gcccatttat cgccgaacag aaacgggatc
     3721 agcccgatct cccgaatggc gggaatgccc gaaatgacca gataaaccgt aatcagcaat
     3781 acacaggcca cagtgatcag acccagtatt agaaagaccc catgtaccgc cgtttcaaaa
     3841 atccgcttgc cgtttttcac gataagcctc ctttggtgcg tccggcccat gggccctccg
     3901 cttttccgag caaaagaata agcgatttct gtcaaatcag aaatcgcttc tatgtcaaat
     3961 ccatgtaaaa tggtatctga agcccttatt tttctttggt ttcccagtca gccagcagac
     4021 ggcgggcttc ttcgctgtct cgatcgatct gggctttcag ctcccccaga ttttcaaacc
     4081 gatgaatgcc ccgcagaaag ccggtcagcg tcagccgcag ctcccggccg tacaggtcgc
     4141 cccgatagtc ggggaaccac gtttccaccg tcaccacggg cgacgaatcc acggtgggcc
     4201 gggtgccgat attggtcacg cccacaaagc gccggccgtc gatatccgca aaggaagcat
     4261 acacgccccg ttctttgggc agatgctccc ccggttccag ttccagattg
//
LOCUS       DAWAEA010000081         4293 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3205_length_4293_cov_2.395706, whole genome shotgun sequence.
ACCESSION   DAWAEA010000081 DAWAEA010000000
VERSION     DAWAEA010000081.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3205_length_4293_cov_2.395706"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2293..2386
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3412..3442
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3826..3912
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          752 a         1258 c         1318 g          753 t
ORIGIN      
        1 gtgcagcaga actgcgacct ttccgccaaa gaggcgctgg aggtcctgtg cgaaatggtt
       61 cacgccgagc ccgtgcagtg catcggccgc cgcttcgtgc tctaccgccg gagccgtcag
      121 cccaagatcg aactgccgtg acggaaaaaa acacacaaac gcagggagac cgaacagacc
      181 cggagcggat cattgccgct ctgcgggagc tgcggtctcc ctttgcgctt tatgagcagg
      241 atattcatca ggccgtggcc cggcggctga gggacgcagg gctgcccttc cttcacgaaa
      301 agccccttac ccccggctgc cgcatcgatt tctggtgcgt ggggatcgag atcaaaaagg
      361 gcaagccccg gcccgccgcg ctgctggaac agctgcggcg ttacgcggtc tcggacgaga
      421 tcgccgcgct ggtggtgctg acccagcgca gcgttcgtct gccagcagcc gtctgcggaa
      481 agccgctggt tcagatcacc ctgaaccagc tgtggggcgt cgccctgccc tgaagcagga
      541 ccgagggagg ttctcattta tggaagaaaa tcaggacttc aatctgctgc cgccctattt
      601 ggtagattcg gacggcgcgc aaagcccgga aagccagacc cagcgcacct acggcaccct
      661 gagctacaac gcccggcgga aatgctggac ggtaaagggc gaccccatgg tgacggagct
      721 gtgcaagcgg ctgtttcccg gcagcgccac ggcccgccgg ggcgaagcgc ggttcacggc
      781 ccaccggcgg gtggtgggcg acctgaactg gctgatgctg cgctatcccc tgctggtgaa
      841 gcccgccgac cggcagcgct gggaaaccgc gctggaggaa gcgcgggagt acgccgttca
      901 caaggaactg gcccagcgga tgccggaaaa ggtgcagccc gaccccacgg tattcaaggg
      961 gcagctgact gactttcagc aggaggggct ggcgtttctg ctgcgctgcg accgctgcct
     1021 gctggcggac gaaatgggcc tgggcaaaac ggtgcaggcg ctggcgtggc tgtgccagac
     1081 cggggcctat cccgccatgg tggtggcccc gccccatctg atgcgcaact gggaaaacga
     1141 gatcgcccgc ttcgtacaga agccggacgg ctcgcccctg cgggtgcatg tgatccacgg
     1201 cctgaagccc tacgccctgc ccccggcgga tatttatctg atgcattatt tgctgctgcg
     1261 gggctggaag gaagccttgc cggacatgga cctgccaacg gtcgtttttg atgagattca
     1321 ggagctgcgc cattcgggca cggagaaata ttccgccgcc agcctgctgg ccgaagccgc
     1381 ccagcgggtg gtgggcctga gtggaacgcc catctacaat cagggcgcgg agatctggaa
     1441 cgtggtgaac attctggact tccacgtgct gggggattat gagagcttta cccgggaatg
     1501 gtgctacggc tatggcaacc gcatcgtgca gaagcccgag ctgctgggcg agcacctgcg
     1561 ttccgagggg ctgatgctgc gccgcaccaa gcaggaggta ctcaaggaac tgccgcccaa
     1621 gcgccgtctg gtcatgacca tcgacagcga cgagggcgtt taccgccgcc tgatggagcc
     1681 ggtgaaccag acccttcgcc tgatgcggga gaccgccgac gccaacgcct cccagcgggc
     1741 gctatgggaa atggaagtag agcggggcga gcggcaggcc acgggcatcg ccaaggcccc
     1801 cgccgccgct cagttcgtcc gggcgctgct ggagggcggc gaaaaggtgc tgcttttcgc
     1861 ccatcatcac gaggtgatgg acatttacaa gcgggaactg aaaagtttct ctcccgcctt
     1921 tatcaccggc agagaaacgc ccgccatgaa ggaacgcagt gtggagcgct tcatgacggg
     1981 ccataccgat ttgtgctgca tttcccttcg ggctgccagc ggcctgaacc tgcagcgggc
     2041 tacctgcgtg gtgttcggtg agctggactg gtccccggcg gtgcacagtc aggcggagga
     2101 tcgggcccac cggatggggc agaccgattc cattctgtgc tattatctgg tcagtcaggg
     2161 cggcagcgat caggagatgc aggacgcgct gggcctgaag gtgagccagt tcgtagggct
     2221 gatgggcgac acgccccagt cggagacaga cgcgctgctg ggggcgcagg aggcccgcca
     2281 gcacgtggaa cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcct gcggggccag
     2401 acagggattg gagcgcagca gccgggctac cggcaggccg aacttcaccg ccagactttc
     2461 cagcgtttct cccgtgccca tggtgtaggt ttccggcacg tcgcagggca cgttctctgt
     2521 gggcacgcac agagtctgcc cggtgcgcag ccgatccaga ttcacgccgg ggttggcgct
     2581 ttccagcgta tggcggctca acaggttctt caactgaata tccgctaccg tctgcccctg
     2641 ctccacctgc acccgctgat ccacggggca ggaaggcgca ggggtttcgg tttccgcacc
     2701 gtccgtggag tctgtgctgc cggaattggt tgttccgccg gtagagccgg tgctgccggt
     2761 ggaaccagtc gtgccgccgg cagagccggt gctgtcggaa tccgtggtgc cgctggtaga
     2821 gcctgtattg ccggaacccg tggcaccgcc cgtggagccg gttgaactgc cgctggagcc
     2881 tgcgttgccc gttcccgtac cggcagaggg ggtatcgtcc tcctcccggg gaatgcacag
     2941 cacgtcgccc accatcagcc gggccgggtg gatatcccgg ttggcggcca gcaggtcccg
     3001 caggggaacg cccagcttat gggcgatcag atagaagctg tcgccccgtt tgacggtgta
     3061 ttcctccgca cagatttttt ctgtatgatt ctgggtcatg gaaaaattct cctttcggtg
     3121 gattctccct ataccctatg cgtcccgaaa gcccccgcat gacagaagcg agcgaaaagg
     3181 gggttacggc tttcccgcgt cccgttccag cgcctcccgc agcagctcga cggcgtgctt
     3241 ttccacacgg gatacatacg acctcgatat ccccagcatc ttggcaatct cgtgctgggg
     3301 atgcaccgca ccgtccttca aaccgtagcg caggcttagg accagccgtt cccggggcgg
     3361 aagggtgggc agggcccgct tcacgccaga cagggtgata cgacggcgga cnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nngtgcccag cggctcgtca agactcacgt ctccctgttg
     3481 ttttctgctc tggcgcagag acatgaggat ttcattttca atacacctcg ccgcgtaggt
     3541 agacagcgac gtgcccgtat ccatgtcgta gctgcgcacg gccttgatca gcccgatgga
     3601 gccgatggag atcaggtcgt cgaaggtgca gccggggatc ttgtatttcc gggctacgtg
     3661 gacgatgagc cgcatgttgt gttcgatcag cttttgacgg gcgtcctcgt cgccctcccg
     3721 catccgcaga agggcctccc gctcttcttc tgcgttcagc ggctttggga agttctgccg
     3781 ccccgaaatg taccccagta aaaataaaca gtcccgaatg atccannnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nngaataacc ccagctcgca gacgccgatc tgctgcagct ccacccgcag
     3961 gtctttttcg aaaagcacgg gatccatcat gtgccagcga tagaagcccc gcatgggcgg
     4021 gcagtcccgg ttgaagtaat cgttgtggaa attgtcgtcc cgggaataga aggggtagcc
     4081 catgaagggc gtgcagtagg tcttttccag catatggccc tgctcgtccg tctggccgcc
     4141 gaaggaccac gcgccgccga agtaatcctc cgtgccggtg gagcactggc tgggaaactc
     4201 cccgtcgccg tcgatgtaga acttcatttc accctcgccc caccagtagc gttccagtgc
     4261 ggagatgccc agataggtgc ggatatagtg ccc
//
LOCUS       DAWAEA010000082         4283 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3219_length_4283_cov_3.375591, whole genome shotgun sequence.
ACCESSION   DAWAEA010000082 DAWAEA010000000
VERSION     DAWAEA010000082.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3219_length_4283_cov_3.375591"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1504..1513
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2400..2409
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3061..3070
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          842 a         1407 c         1237 g          767 t
ORIGIN      
        1 gtcagactgt tccggccctt aggccggaat tggatgcccc gaataaattc ggggcatcca
       61 accgttcggc tcccgcagac cgaagcttgg gccgagaggg agcgcgtgcc cagtgggcac
      121 aactgccgta ggcagttagc gactggcagg cctgagcaaa tacaagagcc gggcacggca
      181 gtgcccggaa cgccgatatt tgcgaataac gcgcagcggg gcgggccgcc tctggagtcg
      241 ccgtgccgcg tgctttcttt ggtactttct ttcgcgccga aagaaagtac tgccctcctc
      301 ggggcggcag acccaccgtc cgcgccggag tcggcgcgtc aatcccctcc gcggaaaggg
      361 gtatgcgcca atgggcgcac caaagggctt tccgctcgcc ctttggaaac cttcgggcac
      421 cgaagctggc gagaaaccca tgacggcaag gcccaatctg taccgggtcg gtacgtcagg
      481 catccagtca gtgcggcccg cactgccgcg cgctccgctt gttgtttgcc tgaacgcctt
      541 cggttcgccc tctgaagttg ggtgagcccc atagggctgc aagccctgct cccgcaccgc
      601 agtcggcgcg tcaatcccca cccctttttc caaaaagaaa ggaatccccc tctatgcagc
      661 atcctcccaa aaaatcctcc cgtaaaaaac acccctggct cctgcctgcc ctgctgaccg
      721 ctttgctggc ggcagccgca gctgtcctcc tgctgctgtg gaagcagcag gccgtttcca
      781 gcgccatgcc ggactcaccc gccgaccggc agaaaaatct ttcgcaaacg gacgcggcca
      841 cgctgctctc cctccgcgtg cagcagctga acggcgagga ttataccctc caatataagg
      901 acggctccct ttggctggag gaaaacaccc ccatcagcga aagcgtcagc gctgaaatgc
      961 tggaagccgt cacgctgatc tccaccaccg atcaggtggc cgccgatgtg gaggaagtcc
     1021 gcgaacacct gacggacatg gggctggagc caccgcagat caccgtgacg gcgacctact
     1081 ccgacggcag gaccgaaact ctggagatcg gcggcgaggt gccggagacc acctaccgct
     1141 acctgcgctg gtccggcgac cccggcgtgt atatgggcga tatcggcatc ggcgaggtct
     1201 tttccatgac ccggaaccgg ctgatcgccg tcgaacagcc ggaaatctcc acagcgctgg
     1261 tggacaccgt ccaactggaa aacgccgccg gaacggtcag cgttcgcttt acggtggata
     1321 gcgccggcta tgcctccgcc gccctgaccg ccccggaaaa ttacccgatg gacgcagaaa
     1381 aagcccaaag tctgcaatcg gccctgaaca atttccgttt gggaacgccg gaagggatgc
     1441 tgaccgacga gacccgcgcc caatatggtc tggagacccc catggccgtg ctgacccttc
     1501 accnnnnnnn nnncccttca tcagcaggcc gggtacgcca gccgcaccaa cgacgaaggc
     1561 caactggttt cggaggaaat ttcagaacgg aactttgttt tcaccttcgg cgactttggc
     1621 agcgattatt tctactcctg cgcctatgaa gggcgtgtat atcaggtcag ccgcctgttg
     1681 acggaggcgc tggtgcaggg cagctgggag tcctggtgtg ccacccggcc cgccgatctg
     1741 ggaaaatctc ctcgctggca gatcgtgctg accaccgagg gcggcacggt gcgcaccatc
     1801 gccgcctacc cgctggatac cttctccgac gcgctgatgg tggacggcgt aattcgctac
     1861 tatgtgcata tcgaagcgct ggacagcgtg ctgggcgaat tcagcggttc tctgacggac
     1921 tgaccgattg agccgccgcg tccgcttcca gcaccgcctt catcaactgc aggagcaccg
     1981 gccccagcag aagccccaga aagcccgtca gccgatagcc cgcatacatg gccatcatgg
     2041 tcagcagcgg ataaagcccc aactgacggc ctacgatccg cggctcggcg atctgccgga
     2101 tcacgatcac gcccacatag gcgcaggcca tgaacacccc catgcccgtg ctgccgccga
     2161 taaacgacag caaactccac gggatcagaa acagccccgc gccgatcacc ggcagcgcgt
     2221 cggccacgcc gatcaacacc cctgccgcca gcccgtaggg aatgccgtaa atcacaaaag
     2281 ccagcgtcag cgccgccgtc acgatcagtg acacggtcag ttggcttttt acttgcccaa
     2341 ataaggccgt cagcaggttt ttccgtacga atccgtaccg ctgccgccag ctcacgggcn
     2401 nnnnnnnnnc gtccgacgaa aaaatccccg gatgcgctcc cggtcggcgg aaaaataata
     2461 ggtgcccatc accgtcagca ccacgcccag cagggcgtta ggcagggaca ttgccgtgga
     2521 aaacgctccg ccggtgaccg ccgccgaaag gcttcccgcc cagcggagaa ggctctgctt
     2581 cagcgcgtcc aacgcggcgg cgatgccctg cgaaacgctg gatggcaata ggcggttcag
     2641 gtcttcgtac catcgctgca aatagggcag ggctacgctg tccacccatt gaatgaactg
     2701 gggcacattt tttacaagcc cggtgaactg gatagcgagc tgccgcagca ggcccgccag
     2761 cgccgctccc accacgccga acagcagcag aatggcaatg ggcgcgccga tcttccggcg
     2821 gatatggaat ttgcccacgc ccctttccag ccgaaggagc agcggttcca gcagcatggc
     2881 gcacaaaagt gctgcggcga agggccacac atacggcagc gcggccccca gcgccgcgac
     2941 ggccagcagg gtgccgccca gcagcgccgc ccggcggggc agaccgttcc aacggcggtt
     3001 ccagtttcgc agtccctcca atgtcttgcc ctcccgatgg tttttgaggc aaaaaaaccc
     3061 nnnnnnnnnn gggccgaagc tatgcttttt tagaaatgtc cgccgcctcc gccgtggctg
     3121 gtgccgctgg aagaggtatg cacgccgctt ccgccgcctc ccccatggct gccggtattt
     3181 tccggcgggt ctggcttgcg cacccgggtg gtggtggtgc gcagataggt gtcctcctga
     3241 tcggtcagct tcatggcgcc gttatcctga taggaataat gataggtgct gcccttcagg
     3301 ctgtagcgcg accgaacgca ggccacgtag atcaggcaca ctgcgaagcc aatgaccagc
     3361 gccagaatga tctcattctt ggtgagggcc ttatgccggg ccgtgagccg ctggccggtg
     3421 accacatcgt agcggtactg tccctcgggg atgcccgccc ggacgtactg ccgcacgatg
     3481 gagatcatct gggacgcgcc ctgcgcgtaa gcgcccgccg acagatagcg ggtcacttcg
     3541 tcgatggcat tttcgatccg tgcgtcggtc atgtagtcga tcatcgcgcc ggtggtagac
     3601 aggtaatgat agcgatcgtc catgtcgatg tagtacagaa tgccgctttt ttcctcatcc
     3661 agcccgaagc cgccctgctc atagaacgcg tcggcgatct gctgggcgct gcccttgtgg
     3721 ctctcgttgg acgtgacgat gacgaaatcc atgcccgttt cttcctgaaa ggcctggatc
     3781 tcctgttcca gctcctgaac ctccgaggcg gtaaacagtt ccgcccggtc gaagacccgg
     3841 tcgccgtccg ccagcgcggc gcttgcccac aggcaggaca ggaccagcag cagggcaaaa
     3901 atccgttgat aaattcttct catcgttctc tgccccctta gaaaagaaaa taagccagca
     3961 cgcagtagag cgcggccaca atcagcccgg ccagcagccc gtgccgccac agccggccct
     4021 gatccaccgg cagcttgccg cacacctgcc cggtacagcc gttcatgacg tagtagtagg
     4081 gatcgtcctt gttcttcttg ttgggatagg tcagcaccca cgtaggcagc agcacgtact
     4141 tggtcttgat ctttttcacc gccgccgtgg tgctggcgga gcaggtctga tactggctgg
     4201 ccgtgtcccg cagcatggag ttcatgtatc cctgcaattc ctgctcgatc tcctgccggt
     4261 agctgtccgc ttccagatcc cgg
//
LOCUS       DAWAEA010000083         4265 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3234_length_4265_cov_2.049169, whole genome shotgun sequence.
ACCESSION   DAWAEA010000083 DAWAEA010000000
VERSION     DAWAEA010000083.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3234_length_4265_cov_2.049169"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          876 a         1447 c         1193 g          749 t
ORIGIN      
        1 cctcatacca gtcctcctcc ggcacttcca gcccggcgat gcagaaaaac tcgtcgctgg
       61 ggaaggcgaa ggtggtgccg agggtacggc ggcattcctc ctgaaagggc gcgatctttt
      121 tcagaagccc ccgggcttcc tcccgggtaa acgtccgcag cgggaacagc ccctcccgga
      181 atttggtcag ccccaccggc accagcgcgg ccgtttcggc ggcgggtgcc agtgaacgca
      241 ggtcgttcag ggtgcgcatc agggcttcgc cgtcattctg ctccgggcag accacgatct
      301 gacagtggaa gtggatgccc gcttccttca gccgggtcag ccgctccatg atgttcccgg
      361 caaagcggtt gttcatcagc cggcagcgca gctccgggtc ggtggtgtga acgctgatgt
      421 acaagggtga agcgtggcgg cggagaatcc gctcgaattc ctggtcgttc acgttggtca
      481 gggtgacgaa attgcccatc atcagggaaa agcgccagtc gtcgtccttc acgtacaggg
      541 tctggcgcag tcccggcggc atctgggcga taaagcagaa ggcgcaatta ttgtagcagg
      601 tgcgcggact cagggtcatg ctctccccga aatgaagccc catgggctcc acctcgtact
      661 ttcggacgga cagatgcagg ggctttccgt cgcgttccag ctccatttcc agctggcggt
      721 tagcgatcaa cgcctgatag tcgatctcgt cgataatgtc ttccccgttg atgcgaagaa
      781 gcacgtcgcc gggaagaatg ccggcccgat gggccgccga accgcgatcc accgcggtga
      841 tctcatggcg cataaagttc ctccttcggc gatcccggcc agcacaggcc ggaaaggccg
      901 ttgtcattat gccctgtttc ggtcaggaag tcaagaaaga gcggcccggc tttcttccga
      961 aggggtgcgg cccgcttcgc cccaatagaa ctggaacaga taaacgccgc cctcttcgcc
     1021 gccctgcatg gaggcgatct cttcctccgc cttttccgac agaacgtcga tcagctctgt
     1081 gggctccgga accaccatgc cccgggccag atatacgtcg tcccgcagaa acagctcggt
     1141 atccagcttc tgctccgtga cgccgaagcg gctcaggcac ttcatgtatt cgggcgaacg
     1201 gaacgtccag ccgccccaga acagcagatt cttgggaacg ccgagatctg tgcgggggaa
     1261 catacggctg tccgcgcaca ggcttccgtc gtagaagtac aggcaatccg gatcctccgc
     1321 cgccatccag tccagcgtgg cgaagggatc cagtatttcc ggattcttcg gctgccgggc
     1381 cagcgcctgc gcggcgggaa cggcgtagtt caccgtcagc gccagcgcca gtgccgtccc
     1441 ggcaaacagc gcggccttcg ccgccgtccc cggctttctc ggcaggcaga gcggcagcag
     1501 tccgaacgcc agcgcggcaa ggggcaaaat cgcctgcagc gccgcccgga cgggaagccg
     1561 tccattccag cccagataca gaaggaacgc gcctgctgcc agcagtcccg ccagcagggt
     1621 cagaccctgc cacagcccat cccgcttttt ccaaagcagt cctaacaggc acaaaagcag
     1681 cgcgccgccc agcgccagca ccgacctcca cgccacggga tccaactccg gcagcgccgt
     1741 caccttccgc cacgcgcttt gcagccgcgc ggcagggtca gccttccacc ggctgtccag
     1801 ctcgtccgcc agccggtgga agctttcttc ggtcagctcg ctgcccagaa agcaccactg
     1861 ggccagcagg ttggcctcct tccgcgtcca gcccgccagc gccagctctt cgtccgtcag
     1921 gcccttgatt ccataatagt ccaccacccg gccgttggcg ttctgccagt tcaggtagtc
     1981 ctccatcccg gcagccttca ttttggccgc gcgccacaaa ggcagggctc ccagcgccag
     2041 cacgatcacg cacaagccgc agagcagcgg cttccacgag cgctttttcc tccggccaaa
     2101 gccgaaatcc cgggccgcca gcggaaccag cgccagaccg cagtacaggg ccaccggcag
     2161 cacgcagatc agccgcaggc cgccgcacag ggccgtcagt cccagcgcca gcaagatagc
     2221 ccggaaaatt tgcccgtcgg tatagcgctc cccgtcgatg gagcaaagct gcagaacagc
     2281 cgccgccccc agcaccgccg ccgtaatggt gaaggtcaca tggcaggcgt aggtcatcgc
     2341 gaaaagcgtc agataaaccg cacccgccgc agcgcccagc gccattgaaa agccccgctt
     2401 ggcaaagcac tgcatcagcg ccttgacgct gaccatgcag gcaagccaga tcagggtaag
     2461 ctgcatccag ctgaaccatg gcacctccgg cgcggccagc cccagccagc gaaggggcgt
     2521 ggacagcagc ggatgcagaa acgcgtgagc ctccgggatc acgccggtgc cgaagcccat
     2581 atactgccgc agaatggcgg tatcgtcatt catggcatat ttcaaatcac caaaaagaaa
     2641 gatcgcggcc atcagcgccg tgctcatgca cagccctgtc agccacggaa tccatgccgc
     2701 ctttttcttc aagctgtttc atcctttccg aatcggtttc agcaaccatt ggattatacc
     2761 aaaaaagccc tttcggcaca agcggcggac gggtgcggaa acccttgtcc acaggcggtc
     2821 acaaatgtaa cgattccccg gaaaaaccgg tcttttccac aggccgcgtt gacattcttt
     2881 tttgtgcaat ggtataatga agcttaccat gcggtctgtc cgtgtggcgg acgcgcattc
     2941 cgatctatca acacaagcta aggagggttc ccctgtgaaa aaactggtct cccttttact
     3001 gatcctgacg ctgacgctga gctgcgtatc ggctctggcg gcttccaaaa gcgcgaaata
     3061 cagcatgagc accgaaaacg tcacggtcct gcaggatgtg ccggatcgcg agatcgccta
     3121 caacaaggac gacctagcgg tcaaccccgt catccccggc gaaagcccca ccaccggcct
     3181 gccggtagcg gacagcgacc gctatatgcc catgctggtg cagatcgcca acgagctgcc
     3241 caagagcaag ttcaagtaca acggcaccaa caccatcagc gccggcgttt actcccgcgc
     3301 gccctggggc ggccagtatg cggacatcgt gtacgaaggc gttctgtacc gcaccggcga
     3361 gacccgtatg accttcctct tcagcgacag cttcgcggaa ggccagcccg tcagcgtcgg
     3421 ccccatccgt tccgcccgtt acggccacgc gctgctgcgg gaagagtggg gcggcggtct
     3481 ggtgttcggc ggcggccccc gtgcccagaa caacaacatt acctctttcc tgaaggaact
     3541 gggcgcgctg taaaagaagg ccgccatgga tctggtgcac accaatgact ataacgacta
     3601 ttcctcccgt gtggacggtc tgcagcgccc caacaacctg aatgcggatg tggtcggcct
     3661 gcgcaacacc atccccgaga ccaccgtagc cacgccccat cccttcctgt tcaccgatga
     3721 gagcccctat accgacggct acgagactgc ctacgccatc aatctggact ggggcagccc
     3781 caactggatc tctcatttct actatgacga aaacgagaac ctctacctgc gctacagcgg
     3841 catcgtgccc tacgccacct acgcggatgc ggaagccgcc gcttccaaca gcaccatcaa
     3901 gaacatcgaa gaccgcgaga tggagcagat gagcttctcc aacgtcatca tccagcgggt
     3961 tccctacgag ttcgccaacg gctcctatga tatgccccag atgcagtccg ccttcaccga
     4021 cggcaccgtg gccaagggaa atgccgacat cttcatcggc ggccgctaca tccccggcta
     4081 ctgggtgcgc gagtccgtct cctcccccac cgtctacttt gacgacaagg gcaacgagat
     4141 ccagctgacc cgcggcaaga ccttcatcgc cgacttccct ccggaagcgc tgctgaccta
     4201 cggtatgaac tccgccaact aacggacggt ttttcccccg agccttacgg cttggggggt
     4261 ttttt
//
LOCUS       DAWAEA010000084         4257 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3241_length_4257_cov_1.712756, whole genome shotgun sequence.
ACCESSION   DAWAEA010000084 DAWAEA010000000
VERSION     DAWAEA010000084.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3241_length_4257_cov_1.712756"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    293..302
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3615..3624
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          847 a         1304 c         1089 g          997 t
ORIGIN      
        1 tcactacctg tgccgcttcg gcccttatcg caaaaaattc atttttagga acctaaactt
       61 tcttcaaaat aactgctcgc cgcaccaccg tgcacttcgc cgtagcagca cctaaacccg
      121 cttcattcaa cgcgcgttct tccctcagca aagctaaaac ccaatcgacc attttccctt
      181 tttttctgca tttttcttta tagaaatgta atggaaatac ttttgcaata cactagtttt
      241 tttttttttt ttagaaatgt aatattttcg taatgtaact aagatataat acnnnnnnnn
      301 nnatataata cttttggagt tgggatggat gcatgtcttc gtcggtttct ccccgaccct
      361 tctttcttaa aacgccgcac gccagcggga tgcggatcac ctggtggcat tctgcggcgt
      421 ttccccaaaa gagaagagct gcctttgcag caggcagctc ttcttgtttt tccccctgac
      481 gggggctttt ttatttcctc ataaagagaa tccccagcgt gttgggcccg cagtggctcg
      541 tcaccgtaga gcctgcgtcc gtctccaaaa tttccgcaaa gggcggctcg tgctgcagca
      601 gcagctcctt caccgcctgt acgtccgcag gatcgcactc ggtatgggta atgaagattc
      661 gatagggatc cacatcgtcc cggccagcca gacgatcctc cacataattc aaaagcacct
      721 gacggaaacg tccgcgatat ttcttggtag gcgccatttt gccgcccatg acctcaatgc
      781 agggtttcag atgcagcacg ttggccccca tcatggtaat ggcgctgcag cggccgccct
      841 tgtgcatata gtccagccga tccagaatga agctggcctc caccttgttg gtcagtcggg
      901 tgcattcctc gtagatttcc ttggcggaag cgtcggcccg ggccatttcc gcgcccttca
      961 gcaccaaaag cccctgcccg gaggacagat tccggctgtc cacgacgtac acttggtcca
     1021 tctcctgtgc ggcggcgcag gcgttctgat agcagctgga aaattcgctg ctgatgcagt
     1081 aatgtacgat cgagtacccc ttttcccgcc aactgcggaa cacgcgaacg tattcttcgg
     1141 tgttgacggc gctggtcttg ggcagctcct tgcattccgc tacatgctga tagatcttcg
     1201 ccggggtcac ttcgataccg tcgcggcccg tgaaatcgcc caggatcacg gaaagcggca
     1261 caatttcgat gtcatagcgg ctgaccagtt ccgacgagag gtcgcaggtg ctgtcggaaa
     1321 tgattttaac gttcatcgtg ttccctccct gttttattgc aggttacgaa atttatcata
     1381 cccattcgga gggtgtcaat caataggctt ccttccaaaa atgcacaaat ttttttcatt
     1441 tcgtctctta tgtccaaaaa aatccccctt ggcataccaa aggggattcg ggaaaatcag
     1501 gaatccttct cgtccggccg caccggcttt tcctgaagca atggcagacg attcagctcc
     1561 tgccgaacac cgccccacag gcagcccagc gccagcgccc caaagccgca ggcccagccc
     1621 atcaggcggc tgtaaaaccg cgcccgcaag agctccggcg tgcggagctc catcacagtg
     1681 gccgtgtcct gccacacgtt ccagaaggcg gcgccaatgt ccttgggctc taccagcacg
     1741 gcgggcaccc actccgggaa ggtgaatacc ggctggatca gcacaacgaa cagccccgcc
     1801 agcgcggcca cacaggcagc atagccgatc accagcccca ctgcccgaag cagcagccag
     1861 cccagcagcc gggcggcata ttcctcccga atcttgctcc gccacgcggc gcatacccgg
     1921 gaaaacagcc ggttcaatat gcggatgagg tacagcgcca gcacccagcc ggaaatgacc
     1981 aacaaaagcc gcaaaggcat cttgccgttc atgctttcct taggcagact gtgggtggag
     2041 ccgctttccg acagctgttt tgcggcgctc tgaaacgcag cccagccgcc cgcacccttc
     2101 accggtttgc cctcaaagat cacgcagctc atgagttgcc tggaagacac cagtggccga
     2161 tagggcacgt acaggctgta ttcctcctga tcgcccaccc gcttttgatc ccgtacaatg
     2221 ccgatcacgc ggtatttttc gccggaaaga acgatctctt cgctcagtgg ctcggcatac
     2281 tgaaacagcg ccacggccag ctgctcatcc agaagggcga ccttttcgcc gtaagtgtac
     2341 tcgtccgggt aaaaatagcg gcccgtgtac agcgcgaaag gctccacgga cagatagttc
     2401 tcgtcgagcc ctacgagccg cgcggaggca gctttttccc cctcgacggt ttccactgtg
     2461 gcgggctggg ccaccgcata cagggtggtc ggctcgcagg agccgtccag ctccaccgcc
     2521 gcataaacga tcaccgccac ggccagcgcc agcagaaaca gcagttttcc gcgttctttc
     2581 caaaatgctt tgattgccat cactgtccgc tccccggtca ctccgtatat tccatcacag
     2641 gcttgccgtc ctccacggtg cggtcggcct tgtcgatgat ctgctgccag cccaggtctt
     2701 cttccacgct gacctgggtg tcactgcggt cgatgacggt gacggcggtc tttttggccg
     2761 tcatgccgga ggaagacagc acgccgccat agttgtactg agccacgaaa acgtactccg
     2821 tgccgccctc agagcgcagg gccgacgcgg gtataatggt ggcgttcttc cgggagcgga
     2881 agacgatctt cgcgtcgatg ctgccgtttt ccatcagctt gctcatgccg cccgcggcgt
     2941 aaagaatatc gtcggtcatt tccatctccg cgtatttgcg gccgtcgctg tcgttaacga
     3001 tctggctgac cttggtgcgg taggtgctgt aatcgccctg cacctccacc ttcgcgccct
     3061 cgctcacgtc ctttttcagg tcggtcacgt ctgcccgaag cacgggctta tcctcttcct
     3121 tggcgatggt atacaggatc ttgtcccccg aatacacgtc gccgcttttg atgtccagcc
     3181 ccaccacata accggcgctg gaggccgtca caacgctcat gctctgctgg gcctcctgca
     3241 gagccaccat gctgtcggaa agctgctcca tttccttcac cagcgcgttg cggttcttaa
     3301 tgtacttgaa tacgtcgtcg gctaccttga tcttcttgtt ttcgtagctg tcgaagaaat
     3361 cctgccgagc ttgatcgaaa ttctgcttgg ccgtaacggc ggcgcggatc agggaggtca
     3421 cttcgctgct ggccttggcg gactgggctt tcgccagcca cgtgctctgg tcatagctca
     3481 gttccagccc ctccttagcg gcggccagac gagccttgct ttccgcttcg gacagcgcgt
     3541 cctgcttggc gatcatatcg tcgtacagat cgttctgcat actctgcttg ctgtttttgc
     3601 ggttggtgat gtccnnnnnn nnnnctcctg cgcctttttg gcgtagctgt cggtcagctc
     3661 ctgttccttt ttgtcgtaat cgttgataac ggcggtcagg atggtatcgc ccttttctac
     3721 atacaaaccg gtctttacgt aaaccttatc caccgtaatg ggataatcct tggccttggt
     3781 cagggtgatc tccgttttta cggggaaata gggcgacagc gccacggcca ccttctgctc
     3841 gatgcgccca acatccgccg tcaccagctt caccttgggg gaggggatgg tctggatggt
     3901 gcgggagaag tacatacaga tggcaatggc cgccgccaga atcaagaacg ccttgagaac
     3961 cttgcttttc ttgctcatgg gagtctctcc tgttatttta gttcgctgag ctgcacgccc
     4021 agcaggatat catcctgaaa atacagatac accagcagca tcggcagaat gtacagcgcc
     4081 gctccggcga aggccacgtc ggcagagtcg gtgttgatct ggttgaacat gacgctcagc
     4141 ggctgctggc tggaatccgt cagatagatc agcggctgct ccaccagatt ccagcagtcg
     4201 gcgaagctca gcgccaccga agcgccgatg atgggccggc agaccggcag caccacc
//
LOCUS       DAWAEA010000085         4237 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3264_length_4237_cov_2.397896, whole genome shotgun sequence.
ACCESSION   DAWAEA010000085 DAWAEA010000000
VERSION     DAWAEA010000085.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3264_length_4237_cov_2.397896"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1338..1347
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3186..3195
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3687..3696
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          874 a         1340 c         1193 g          800 t
ORIGIN      
        1 ccttgatatc cgtgttgggc ttttcgtctg taaccgagac cccgctgacg ccggcgtcct
       61 ctccgttttc gtaagacgtg taagcctcaa tggtgctgtc atctatcaca tcctctgtga
      121 cctcttccgc catagcgacg gcaggcagca gggatactgt catccatacg cagatcaaca
      181 gggccagtgc ccgttttacc gtttctcttt tcactgtgtg cgctcctctc cggtgtggtg
      241 attctctgtc actattttcc cgtcactgaa atagacggta cgatctgtat aaagcgccat
      301 ctgtggatca tgtgagctga gaacaaaggt ggtgttccgc tcccggaaga tctcacacag
      361 cagctccatg acctcctggc cggtgcggct gtccagattc ccggtaggct cgtccgccag
      421 cacgatggag ggctccgtaa taatggcgcg ggcgatactg acgcgctgct gctgaccgcc
      481 tgacagctcc gcgggccggt gatctacgtg tccgcgcagt cccacgcggc tcaatgcctg
      541 ccacgccagc cagttccgtt catcctccgg cacgccccgg atggccagtg gcagcgccac
      601 attctccaac gccgtgtatt ggggcagatt gttgtagctt tggaacacaa agccaatata
      661 gcggcggcgg aaccttgtcc gctgctcctc gtccatagcc tccaaatgat gcttgcctcg
      721 gacaatgatc tcgccctccg tgggcggctc aagacctgcc agcatattca gcatggtgga
      781 cttaccggag ccgctgcggc ctgcgacaca gacaaattcg ccttgcagca cggacatgtc
      841 cacgccatcc agcgcacgaa ccgtgttgcc gccgacggtg tagtgcttct tcagccccac
      901 cgtcctgatg atcgtttttc gttcactcat agttctctct cgctatctca aatgtggagg
      961 gcggcagcac atagttccgc tcctccgccg tcaggccgaa tttgatcttg atgcgctcca
     1021 gcttccgcac ccacgggtcg cccagcgccc acagcaccag aaacgtacac agcccgtgac
     1081 agaaatcgaa gaaaaagctg gatagatagg ccgcgcctat cgtctgccat gtaagggggc
     1141 ttacataccc gatgatgaaa tacaggttca tgatccagcc gtacaaaaag ccggacagga
     1201 aaccataaag gcacagccgc aggggcgcat ggtcggtgag ctgccgcacg aaatgcagca
     1261 ggtcgcccct gttcgtattc tccggacaca gcctgtccca cagcttcgcc gtgaagtggg
     1321 ggcggcttac tcccttgnnn nnnnnnncat tttgcaggat acccgccaga aagcccacca
     1381 gcccccatgt gaacatctgc catggcgtcc acggcccctg tccgaaaatg aagttgctga
     1441 caaaggcggt cagtgcgccg gtaagaaagc ccgcctccgg cccgaaggcc gccgccgtaa
     1501 tgataatgat ggcggagcag ggcttgaagt tgggcagcgg gatgatctca aacaccgtgc
     1561 ggcctaccac ggcaatggcc gccatcactg caatcggcac cagctcgcga gcctggggct
     1621 tcttcttatc gaataccagt atgaagggga tggcggacac cagcaggaac agcaggccca
     1681 gcagaatatc cgagtttaat atgatgtagt cccagaaggt acgggagcct accggattga
     1741 ccgccggacg gtggctgtgg taatagccga aggccaccat cagggcgggc agcaccagaa
     1801 ggatcaccca cgcggcggct ctggttcttt tgctgacaaa gagcttcgac acgacgcgat
     1861 cacctcctcg caggtaatgt ccatggggaa gtgctccgcc gcgatgcggt tggcgtcggt
     1921 ggtatagaag cggttgcccg cgaagaaggc atgtggctcg ccctcggaca gcagcagtcc
     1981 gtcaaacatc aaggcgcagc ctgtggcgta ccgggcggcg aattccacat cgtgggtcac
     2041 caccagaata gacgcgccct cccggcacag tgccagcagc agttgcgcca gctccgcctt
     2101 ggcataggcg tccagcccct tggtgggctc gtccagcagc agcaccgtgg cctcccgcaa
     2161 cagcagcttg cccaccgcca gacgctggat ctcgccaccg gacaggtcgt agggatgccg
     2221 cgccagcaaa ggcgtcagct ccagcctctc cgccatctgc acggccttag ctttgtcctg
     2281 ttcggcactg tccagcagct ccttctccac cgtgtcggcc acaaacagcg accgggcgtt
     2341 ctggggcagc atacacagcc gctgaccctt tgccagcttc accttgcccc gatagggctt
     2401 ccgaaagccg gccagtgcct gcatcagcgt ggttttgcct gcaccgttgc cgcccagcag
     2461 gcatagcagc tccccctgat gaagcgttac atctgtgccc cgcaggacat ccttgccctt
     2521 ttcggtatag cggaaccaaa gctccttgcc ctctaaaacg gcgaggtgct ttgcttcctt
     2581 ctcaggcttt gcggtttcag gcgcggaagc ctccgccgcg atgggcagca gctgctccaa
     2641 gcggcggcgg ccgtcccgta ccgtcagggg cagatcctcc gtttgctcca attcgctgaa
     2701 aatacggacg gcggcgggaa gtccgaaata gatacggctc ttttccgcgc tgccggatag
     2761 ctgccgcgcg atctcagccg gtgcgccgtc cgaaaggata cggcccttat ccatggtcac
     2821 cacacggtca gcggcgggga atacctcgtc cagccgatgc tctgtcataa ggatggcaac
     2881 gcccagctca cggttgatcc gctgtacggt cgccagcaga ttccgggcag ccagtgggtc
     2941 cagcatggag gtgggctcgt ccaaaatcag cagcttcggc tccatggcca gtgccgaggc
     3001 cagattcatc agctgcttct gcccgccgga cagctccgtg gtcttggcac ggaaccacgg
     3061 ctccatgccg aagaaagcgg ccatctccgc cacacggcgg cggatcaccg gcacgctcag
     3121 cgccatattc tcaaggccga aggccagctc gtgccatacg aaattggtga cgatctggtt
     3181 atccgnnnnn nnnnnctaat ggcctccttt gtttcaagga taccgggcag gaacatcagc
     3241 acagcgtaaa agacaaacat ggtatagctg gcactgccca gcagcaccgg cgtcatggcg
     3301 ggatagaact ccatataccc gtgaccgaag gcgcgggcgg taatgcagat gcctgccact
     3361 gccagaatga ggcccagcgt ccgggcgtcc cggccatcga aacggaacag gtggaaggtg
     3421 gtacgccgct tggtaccgta gccccgggct ttcatgctgt ccgccgtttc caccgccgcc
     3481 tccatgctca tggtcagcag caccgacagg ttccgcaggg cgctgcccag cctttgcagc
     3541 aggcgtgtcc cctcatcgca gagcatcgcc tgcgcctgac ggatctcgcg gcggttctgt
     3601 tggagctgtg ggatgaaccg cagcgtcatg gtgatcagca gcgacgtacc gggaaagggc
     3661 ttgccgaaca ggtacaggaa cttatcnnnn nnnnnnaaca cataatggcc gcgcacaggg
     3721 agaagccgga caccagaccg tagcacacgg cttccagcgt gtaccacagg tgattgatgg
     3781 taaaaagcat ggtcacgccc cgatggttga atagcggatt ggcgagggcg atcagcaaaa
     3841 acataggccc cacaaaccgc atggtgtggc cgtaggccct ccagcctcgc agccggatgg
     3901 aaaaccagct ggcgccgaga aaggacagcg ccaccgtaac ggggtgcatg catcccagcg
     3961 tcagacccag caccagcacg aagtacgcca tactgaccgc cggatgcagg ttcccgaaga
     4021 aacgcatccg ggtatcagtc atggttatcc ttccaatcct gagcaccttc ccaccaaccg
     4081 ccatctagat caaggccaag gtcgcaggtg taattccact caatgacctc gccgggcttg
     4141 acgtagtaca cgccgcaacc gtagttggga taccagtcgt tgacacagta catccagccg
     4201 ctccagcggc cgccatcgaa ctcatagagg ttgttga
//
LOCUS       DAWAEA010000086         4189 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3306_length_4189_cov_1.801161, whole genome shotgun sequence.
ACCESSION   DAWAEA010000086 DAWAEA010000000
VERSION     DAWAEA010000086.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3306_length_4189_cov_1.801161"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2258..2456
                     /estimated_length=199
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          837 a         1270 c         1067 g          816 t
ORIGIN      
        1 gcgttaaaca gcgtgctctt tcccacgttg ggcagtccta cgataccaag tttcatagcg
       61 ttcctcttct cctcagtaaa tccatggtaa ttcattcttt agtatagccc gaacccataa
      121 aaaaagcaat ccgaaccgcc gttcttttcc tttttgcccc atctttccct tgtcaatcgc
      181 tcttttcctt cctataatag agtgactctg cgccgcccgg cgcagccaaa tccatctcgg
      241 aggcgacagc catgaaactg cgttctcagg aagtccgcgc cctcgcgccg gaaaacgatc
      301 ctctgaaaat cggcatgggc tggtcggcgg acgatctgtc caagccccaa attttgatcg
      361 aaagcacctt cggcgacagc catcccggca gcgcccatct ggatcagttt gtccgggaat
      421 ccgcccgcgc ggtcaacgag cacggtggaa aagccgcccg ttatttcgcc acagatatgt
      481 gtgatggcat cgctcagggc catgacggca tcaattattc cctgccccac cgggaagcca
      541 tggtcgatct ggtcgaggcg caggccaacg ccagcgtcta cgacggcggc gtgttcatcg
      601 ccagctgcga taaatccgat ggaggctcat accctgccga aggaatacac cgtgaacgac
      661 cctgcctgca agatcaacga actgctgacg ctggaacaga tcggcaagtt cgacgcatgg
      721 gaaaagaccg gcgtgatccc ggagaagcag ctggactact ataagcatca cgcctgtccc
      781 agctgcggcg cgtgttcctt tatgggcacc gcctccacca tgcagatcat ggcggaagcg
      841 ctgggtctga tgctccccgg cacggccctc atgcccgcca ccgcccccga gctgaaacag
      901 gccgcttacg acgcgggcgc gcagctcatg aagctggtgg aaagcggcct tcgggcaaag
      961 gatatcgtca cccaaaagag ctttgaaaac gccatcatgg tgcacgcggc catttccggc
     1021 tccaccaacg ccaccatgca cctgcccgcc atcgcccacg agttcggctt cgagctggac
     1081 gcggacgctt tcgaccggat gcaccggggc gctcattacc tgctgaatat ccgcccctcc
     1141 ggcgattggc ccgcccagta tttctattat gccggcggtg tgccccgggt catggaggag
     1201 atcaagtcca tgctccatct ggacgttctc accgttaccg gcaaaacgct gggcgaaaat
     1261 ctggaagacc tgaaacgcgg cggcttctat gagcactgcg acgagcttct gcaaaagaag
     1321 gcccatgagc tgggcctgtc cattgcccgg acggatatca ttcattcctt cgaccatccc
     1381 aagggcaccg acggcagcat cgccattctc aagggcaatc tggcccccga aggcagcgtc
     1441 atcaagcaca ccgcctgtcc gaagaatatg tttcaggcca ccctgctggc ccgtcccttc
     1501 gacagcgagg aagaatgcat ccacgccgtg ctgaacggtc aggtgaagcc cggcgacgcg
     1561 atcttcatcc gctacgaagg cccccgcggc agcggaatgc cggagatgtt ctataccggc
     1621 gaggccatct gtgcggatcc caagcttgcc tccagcgtgg cactcatcac cgacggccgc
     1681 ttctccgggg ccagccgcgg cccggtcatc ggtcacgtca gcccggaagc cgccgcaggc
     1741 ggtcccatcg cgctggtgga ggagggtgac ctcatccgca tcgacgttcc cgcccggaag
     1801 cttgccatcg taggcgtgaa tggccagccc aaaacgcccg aggaaatgga gcagattctc
     1861 caggaacgcc gggcccgctg gcagcccaag ccgcccaaat acacccgcgg tttgctgaag
     1921 ctctactccc agcacgccgt cagccccatg aagggcgctt atatggaatg agtccgtctt
     1981 ttaggcagaa aaccctttgc catagtgtct ctcaagatcg caacttcttc cgatttgcgc
     2041 tgcaatgcgg tatgctcttc gttttttcga attcctgaaa aggctactgc agttcggagc
     2101 gtgcgaccaa ggaaggccta aggatgacta gcctcagacc gtgcagccaa gcgcaagctc
     2161 agcgcagggc ttggccatcg tacactgtgg ggcagaccac aatagtttct ggcgaaaaag
     2221 ctcgcctagg gtaatctttt gataagctgc aaaaaccnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntgct
     2461 catcacgtag ccgaagcgct tcccatactt cacgtaagcc catccgtcgt ccaggtattc
     2521 ccgaacgatg atcttcgtgc cgtagggaat gttgccgatc ttgttgccgg tattgctctt
     2581 gggcgtactc cgcagattca gggacttccc gtctttcgta tacacatagc gtgtatccgc
     2641 cagcgcaatc agcggcagca gcgtcgtcgc aacaatcacg acaatgcaca ggaacgcaag
     2701 cgtctttttc atccgaagcc tctccttccg caaaccggat ttcccgcagg gaaaagaaca
     2761 aaccctgcat ggcgaatgtt ttgcttcata atattaacat atttttacaa aaaagtcatc
     2821 actttatcga aagatttgtt tcgtttctaa ttccttttgt aaaataaaaa caacattatt
     2881 tcaactatcg tcctccgcaa agaatcccca attcatttcg aatcacaaag gaatcccaac
     2941 tcttcgtttc tacaaagaga ccgccccatc tgccaaaagc agcttggggc ggtttctctt
     3001 tatgcgtttt tcagcaccgc ttcatcactt cggcgaagaa gcagatggtg tccttgagct
     3061 gggccacggg gatgcgctcg ttggcgttgt ggatcagtcc ccgctgctcc ttggacatgg
     3121 gcatgccgct gaaacggtat acatgctcgc tgatggggcc gtagtggcgg ctgttgctgc
     3181 ccgctagcat cagatagggc gagaccagca cgccgggata ggcggcgccg atggcgctct
     3241 tcagccggtt ccacggctcg tcaccggtct gggagactgt gctgggttcg ccgccggaga
     3301 cgagcttcac ctcgacggac ggatcgtcca cgatggtctt cagccgggcg atggccgcct
     3361 cgatggtgtc cccgctgatg agccgcaggt tgatgcccgc gtgagcagtg gagggcagca
     3421 cgttaaaggc tttggagccc tgagccatgg tcagggcaca ggtggtgcgc accagcgcgt
     3481 tcagctcgcc tccagacctg cggcacacca gatccagcag cggcccgaag caccacaggt
     3541 tggcgaacag cagcttgtag ccaaagctgg attcccgacc cagaatatcg aacatctccc
     3601 gggcgggagg ggtcagggta aagggcatgg cgtgatcctg aatgcggctc agcgcccggc
     3661 ccagaatgcc cagcgcctga cggctggccg gagcgctggc atgaccgccc ttgccggacg
     3721 cggtcatgtc cacgctgacg gtcccctttt ccgccgttcc gatcagcgcc gcgctcttgc
     3781 tgacgccggg aaatacccca tccacgatag cgccgccctc gtccagcaca aattccggat
     3841 gaatgccgcg ctttttcagt tcttccacaa tggcaggcgc gtcgccgccg aaaatttcct
     3901 catcgccgcc aaaggcgaaa tacacgtcgt tttccggcac gaagccctgc ccaatcagca
     3961 tttcagcggc ttccatcacg ccgtgcaggg ttcccttggt gtccagcgtg ccacggcccc
     4021 aaagctcccc gtccttcacc accgcgccga agggcggctc ctgccattcg ttttcgttca
     4081 ccggcaccac gtcatagtgc gccatcagca cggaaggctt cgcactgctc ttgcccttcc
     4141 agtggtacag cattccccgc cggcccagaa tttcccgagg gcaggtttt
//
LOCUS       DAWAEA010000087         4159 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3332_length_4159_cov_2.315789, whole genome shotgun sequence.
ACCESSION   DAWAEA010000087 DAWAEA010000000
VERSION     DAWAEA010000087.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3332_length_4159_cov_2.315789"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    290..299
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3510..3673
                     /estimated_length=164
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          752 a         1149 c         1276 g          808 t
ORIGIN      
        1 aatctgggag cggcatcgtc accgctatga gttcaacaac gattaccggg agcgctttga
       61 aaaggccggc atggtcgtgg ctggacgcaa tcccgagcgg gatctggtgg agatcgtgga
      121 gctgcccgat catcctttct tcgtagcggt gcagtatcac cccgagttca agagccgtcc
      181 caaccggccc catccgctct tccgggagct gatcggcgcg gcgatgaagc atcagaaaga
      241 gcataacgcc tgaccggcgc ggcgcgggtc tccaaaatgg gacctgcgtn nnnnnnnnna
      301 atggaatagt gaaaatcagg atgaagttgg atgttccggc ttgatccaac caactgatat
      361 tattcaacgg cgtggaagga gaacggaaat gcataactct caaggactgg aaaaggtgct
      421 cagtcttcgc caggaaattc tggcgggaga cgagaagcgc ctgaaggagc agcgggatgc
      481 cggaaaactg accgcgcgcg agcgcattgc caagatcgcc gacgcgggca gctttgtgga
      541 gctgtttgcg ctggtgagcc ggaacgatga gggcgcgggt gtggtcaccg gctatgccac
      601 catcgaggaa cgcccggtgt acctttttgc tcaggaattc accgtgcgcg gcggtgcgat
      661 gggccagctg caggcagcaa agatcgtcaa gctgctggaa ctggcgcaga agaccggcgc
      721 tcccgtgctg gcgctgatgg acagcgctgg cgtgcgcatc gacgagggtg cgcaggcgct
      781 gagcgcgtat tctgaaattt accagaagat ggcccggatg agcggcgtgt gccccatggt
      841 ggcgctggtg ctggggccct gtatcggcgg cgcggcgctg ctgtctcagt tggcagacgt
      901 gtccatcgtg gctgaaaagg taggccagct gatggtcttc ggaccgcagg tcatggccgc
      961 catgaacggt gagcaggtgg atgcggagaa gtttggcggc gcgaaggtcg ctgctgaaat
     1021 gggcgcttgc gctctgaccg ccgccagcga ggacgaggcc ctgctgaagg cccgtcaggt
     1081 gctggcgctg ttgccctcca gcaatctgga agacgcggag atcgtggacg cggacgacct
     1141 gaatcgtctg ctgcccgcca tcgatcctgc tgacgcggac gctctgctga acgccatggc
     1201 cgatcagggt acgctgctgg agctgtacgc ggcttacgag ccctccgtga aggtggcgct
     1261 ggcccgtctg ggcggctaca gcgtgggtct ggtggccgcg aatggcaagc tgaccgccgg
     1321 tgcgctgcag aaggccgccc ggtttgtgcg cttcatggac tgctacggca ttcccgtggt
     1381 cagcctgctg aacacctccg gtgtggaagt gaacagcgtg aaggagcagg gctggctgtt
     1441 caaggcgcag agccagctgc tttacgccta tgccgaagcg accgtgccca aggtggccgt
     1501 cgtgaccggc gacgccatcg gtcaggctta cgtcgccatg ggcggcaagg ccatggccga
     1561 tgtgacctac gcgtggccct ctgccgtgat ctccgccctg acccccgagg ccgccgtggc
     1621 ggtgctttat caggacgaag tgaaggctga taaggaactg agcgtggaag aagcccgcgc
     1681 caagtatgcc tccgaatatg tggagaacgt ggccggctgc ctgaacgcgg ccaagcaggg
     1741 catggtggac gatgtgatcg aacccgccca gacccgcgcc atgctgatct ctgcgctgga
     1801 aatgctcatg agcaagcggg attccaatcc ccccaagaag catggcaacc tgcccatgta
     1861 acgccgaaag gaagggttta ccatggaaaa attcaccttt ggtttaggcg tggcaggtct
     1921 gggcatgatc gtggtatttg cgggattggt gcttctgatc ggctttatca cgattttggg
     1981 cgtggctgcg aaggaacgca aaaagcctgc gcccaagacc gagcaaaaag cggaatcggt
     2041 cgctgctccc ccggtgcaga cggccgtcgc tgcggacggc gtgtccccgg aagtgatcgc
     2101 cgccattacc gccgctatcg ccgccgtctg ggaaagcccc aacgggtttg tggtgcgcca
     2161 tgtaaagcgc gtttccaagg cgcccgcctg gaagcgtgcc ggacgtgacg aacagaccta
     2221 cagccgttac taatcattca ttgtttttgt aaggaggata caccccatgc gtcagttcaa
     2281 cattaccgtc aatggtgttc agtattccgt ggccgtggag gaagtgggcg ctgcgtccgc
     2341 tcctgtggcg gctcccgtcg ctgctcccgc ccccgtggct ggcggtgaaa agatcaacgc
     2401 tcctatgccc ggcaacattc tggacgtgaa ggtcgccacc ggcgccagcg tcaagaaggg
     2461 cgacgtgctg gtcattctgg aagccatgaa gatggaaaac gagatcatgg ctccttgcga
     2521 cggcaccgtg aagcaggtgc tggtcaacaa gggcgcgacc gtcaacagcg gcgatcccct
     2581 cgtggtcatc ggctgagatc gacttcgttc tttcccgtat tttgtaaaga aagtgagttg
     2641 atttcaaacc atgaacgttg gcgagagctt gttgaaactc tgtcaggatt ccggtattta
     2701 ttccctcctg caggatccca agccgctgat tatgatcggt ctggcttgcg tgcttctgta
     2761 tctgggcatc gtcaagggct atgagcccct gctgatggtt cctattgcct ttggtatgct
     2821 gctgaccaac cttccgatcg ccggtatgta ccacgaggaa ctgttcgcgg gcggtcatgc
     2881 caactggaac ctgttcggcg gaagtattct gttcaattct tccaacctga tcgatgcggc
     2941 gcaatacaca gtgacagcgc agggttccat tctggacgcc agcaatcagc tgctgggcac
     3001 catcgtggat accatctccg gctccgcagt ggccttcaac gcctccggcg cgctggtggt
     3061 ggatggtcag gtggcctatg attccgtacg gatcatcatg aatgccgcgg gcgcttatct
     3121 ggcggacgga catgtgtata gcctgctggg cgtcgaactg gcgactgccg gtgaagtcat
     3181 cactcccggt ctgctggact acctgtatct gggcgtgaag cttggcatct atccctgcct
     3241 gatcttcctc ggcgtcggcg cgaccacgga cttcggtccc ctgatcgcca accccaagac
     3301 cctgctgctg ggcgctgccg cgcagctggg tatcttcatc acctttatcg tgtgctactc
     3361 cgtcatgggc tttacgcctc aggaagcggc ttccaccggc attatcggcg gtgcggacgg
     3421 ccccacggcc atctacctga ccggcaagct ggccccgcac ctgctgggcc ccatcgcggt
     3481 ggccgcttac agctacatgg cgctggtgcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnagcgctc catcgtgatg gaacagctgc ggcccgtttc caagaaggaa
     3721 aagatcattt tccccattgc cgtgaccatt atcgtcagcc tgttgctgcc ctctgcggct
     3781 ccgctggtgg gctgtctgat gctgggcaac ctgtttaagg agagcggcgc ggtggatcgt
     3841 ctgaacaaga cggcccagaa cgaactgatg aacatcgtga ccatcttcct gggcatcacg
     3901 gtaggcgcga cggcgacggc ctccaccttc ctgcagccca agacgctggg catcatggtg
     3961 gtgggcgtgg ttgccttcgg cttcggcact gccggcggcg tgctgctggc caagctgatg
     4021 aacaagcttt ccggcggcaa gatcaacccg ttgattggtt ccgccggtgt gtccgccgtt
     4081 cccatggctg cccgcgtcag tcaggtggag ggccagaagg ccaatcccgg caacttcctg
     4141 ctgatgcacg ccatgggcc
//
LOCUS       DAWAEA010000088         4106 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3386_length_4106_cov_2.432980, whole genome shotgun sequence.
ACCESSION   DAWAEA010000088 DAWAEA010000000
VERSION     DAWAEA010000088.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3386_length_4106_cov_2.432980"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    314..453
                     /estimated_length=140
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3675..3684
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          761 a         1166 c         1270 g          759 t
ORIGIN      
        1 gcacgatatc gtcttcatcg acgacgacgc gcgttatatg tacgacctcc ggcatgaggt
       61 ccgtttcatc aatgccggcg aatacgaact ggccagcgac cagatgttca tgggtttcct
      121 gtgcggcatc gtgtcccaga acttcgatct gggtctggtt ttcgtcgacg cgttcaagaa
      181 gctcttcaag gcggatctgt ccacctctga atggttgttc gagcgtctgg agaccatcag
      241 caagaaccac aatgtggact tcgttctttc catcagcgct gatgtggaag aactgcccgc
      301 gttcatccag aagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccgttag agcgccgata cgcccggacg
      481 gccctgccct gtacgctcca cccggtaaaa gccgatcaga ttggtggggc gggtatggtt
      541 gtagacctcc tccacggcgg aaaaatcctc caattcaaag cggatgctca ggccgcagcc
      601 aacggccacg tcccggggcg tggtgatgat ctgcacgcgt atgcccgccc ggcggagggc
      661 agcttcaaat ttcatcacct gctgccggga gcggaaggac gcgatgccga atgtttcctg
      721 cataggttca tttccccctt tccttctcat acagtatacg gcgcggggcg cgcaagtgtg
      781 agaggcggaa atgaggaaga aaaaagatga tctatctgga taacgcggcc acctcctatc
      841 ccaagccgcc catggtcgtc cgggccatgg cgggcacgct ttcccgtctg ggcggcaacc
      901 cgggacggtc ggggcacaag ctgtcccttt gcggcgggcg ggtgatcgaa cactgccggg
      961 aaatactggc ggaggagctg gaagccccgg cggcggagca gatcgttttt acctccgggt
     1021 gtacggaggc gctgaatctg gccattcggg gaagtctttt ccggggagac gaggtgctgt
     1081 gcagccacgc agagcacaat gccgtgatgc gggtgctgaa aggactggaa cagcagggcg
     1141 agatcaccgt gcgggtggcg gagccggaca gccggggact gctttcgccg gaagctctgc
     1201 ggcaggcgac gaccccaaaa acggcgctgt gcgtcttgaa ccacgccagc aatgtgaccg
     1261 gcgttgtcca gccagtgcgg gaactggcgg acgcgcttcg ccccatgggt atccccgttc
     1321 tagtggacgc ggctcagacg gcggggatcc tgccggtatc gctgaaaaaa ctgggggcgg
     1381 atatgatcgc cctgccggga cacaagggac tgctggcccc ccatggcacc ggggtgctgg
     1441 cgctggggcg ggggatgcag ccccggccgc tgctggcggg cggcacgggt tcccaaagtg
     1501 agagcatggt gcagccggag tcgctgcctg accgttatga aagcggtacg cccaatctgc
     1561 cgggaattgc cgggctgctg gccggggcgc ggtttgcctt ccggcacaga gcggagatcg
     1621 aggaatacga acgcgggtta gcccggcgga tgcgtcaggg tcttgggcaa attcggggat
     1681 tgcggctgct gggaagcgat ggcgcgccta tggtgggcgt ggtgagtttt gtgcccatga
     1741 gacgggacgc gggggaggtg tgcgaccgtc tgtcggagga aggcttcgcc ctgcgggcgg
     1801 ggctgcactg tgcgccgtcc atccatcaat ggctgggaac actggaaacc ggcgcgatcc
     1861 gggccagcgt gggcatttac aacacggagg aagaaataga ccgtttcgtg gaaacggtgg
     1921 aaaaattggt tcgataaaaa atgccccggc attcgttttt ctgacgaatg ccggggcatt
     1981 ggaatgaaac ggggttattc ctcctgcgga aggagatccg tccagccgaa ggggtcgggc
     2041 aggcggcccc actggatgcc ggtcaggttg tcgtacaggc gctgcgccgt ctcgccgatc
     2101 tcaaaatggt tgatgatata cggcttgtcg ttgtagtaca gctcgccgat agggcagatg
     2161 accgccgcgg tgcccacgca ccacgcttct tccagcgtgc ccgcatcggc ggcttccgcc
     2221 agctcgtcga tgctgatgag ccgttcttcc accggcagac cccagcttcg catcagttcc
     2281 agacaggaca tacgggtgat gccccgcagg atggagccgg tcagcatggg cgtgaccacc
     2341 ttgccgccca gcttgaacat gacgttcatg ccgccgcctt cctccacgta tttgtgatgg
     2401 atggcgtcca tccacaaaac ctgcgagaaa cccttggccg tggcgcgctc tccggcccgg
     2461 ttggccgcgc cgtaattgcc gccgcatttg gcttcgccgg tgccgccccg gacggcgcgc
     2521 acgtcttcgg tctccaccat gattttcacc ggcttcaggc cgctggcaaa atagctgccc
     2581 accggggaaa gaatgatgac gaacatcgcg tcatgaacgc catgcagccc cagctttggg
     2641 tcgttggaga acaggaaggg acggatatac agggacgtgc ccggcgcagt gggcacccag
     2701 cgctggtcca gcgtgaccag cgtgcggatg gcttgcaggg catcatccgg gttcaactgg
     2761 ggcagcccca gccggtcaca ggagcgattc agccgggcta cgttttccca gggacggaac
     2821 agctgcaccc gtccgtcagg acgacggtaa gccttcagcc cttcaaaaac ctccgtgccg
     2881 tagtgcagca cgctggaggc gggggacagg gtcagggggc cgtagggcac gatgcgcgcg
     2941 tcatgccagc ccctgtcctc ggaatagtcc atgaggaaca tatggtcggt gaaatagttg
     3001 ccgaagccca gcttttcagg ctcggggaga atgccgtgaa cgggagaatg agtgattttg
     3061 atttccataa cgcaaggctc cttttgaatg aatttgaagt gactgcggaa acgggatcac
     3121 agctctttca ggtgctccat gttctggcga atgcccgcgc agcactggtg gaaggtctgc
     3181 ttttcttcgg cggtcagggg cagctcgatc acctgctcca cgccgtttgc gcccagtaca
     3241 cagggaacgc ccgcaaacag gccgctttcg ccgtattcgc cgcacagctc catgctggcg
     3301 ggcataacgg ccttttcatc gtgaaggacg atggaggcca tccgggccgc tgtggtggcg
     3361 atgccatatt ccgtgcagta cttgccggag aaggttaccc agccgccgtg aatggcttcc
     3421 ttcgtcaagg cttcccggtc gaagcggaag cgctcgtccc ggcgcgccca ctcctccagc
     3481 gacattcccc ggaagctgac gcaggaccac ggggcaaact gctgattgcc gtgctcaccg
     3541 agcatatagc aggtgatgga cttatgatcc agccctgtct gacgggccag cgcgctgagc
     3601 atccgggagg tgtccagccc ggtgcccgtg ccaaagactc gtcccttggg cagccccagc
     3661 agcagcgcca gctcnnnnnn nnnnccccgg acggcgggaa tggtgaagtc catttccgtg
     3721 acccggttgt gattgccgcg cagcagttcg atcttgccga cgctgttgac gatcagatcg
     3781 cattcgccca gatcggaaaa atctccggca cgaacggtga cccggtgctc aaaataggcg
     3841 acggaatccc ggagatcctg cacctcgctg gcgaccttct gttcgttctg atccaccagg
     3901 atcagctcgt cggcaatgcc ctgcaccgcc aacgaatagg cgacgtgcgc acctacgtgc
     3961 cccagcccca gaattgcgat gatgcgtttc tttgccatgc ttgcgacctg cctttctctg
     4021 tctttacgga ggataggatg accagaattc ggcgcggaat ttctcctgaa aaaaggaaaa
     4081 gcggccccgc ctgctttggc gaagcc
//
LOCUS       DAWAEA010000089         4102 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3391_length_4102_cov_1.878181, whole genome shotgun sequence.
ACCESSION   DAWAEA010000089 DAWAEA010000000
VERSION     DAWAEA010000089.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3391_length_4102_cov_1.878181"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    256..265
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1415..1424
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          743 a         1384 c         1133 g          822 t
ORIGIN      
        1 agcttcagca ggccatatcc gtcgtgttcc tgcagagcgt ggccgcattc gtgagccgca
       61 acgcccaccg ccgccacact gtcggagccg tacaccgagt cgctcagccg cagggtattg
      121 ctgcgggggt cgtaatggtc ggtcagggag ccgttcactc gactgatggt cacgttggtg
      181 ctgccgcccc ggctgagcag catccgcgct gctgcttctg ccgtcaggcc gcttttcgtc
      241 ctgacccggc tgtagnnnnn nnnnnctctt cacattgaac tgcgcgtaca gacccagcag
      301 caggcccgga atgaccaaaa tcatcgtcca gtcataataa aagtaaggca tatttttcct
      361 ccttatcgga acatttccgc attttaccgc acgatttcaa cgcccagcag gccgcacagg
      421 atcaacgcct gttccgcact gatccgaagc ccctgcaggt cggacggcgt cattcggatg
      481 ccgtccagct gacaggtgct cacgtccgct cctttcaagg gcgtatggct ccattgagag
      541 cgggaaagga cgttttcttc cagacgaatg cggcccagtc tgcactccgt ccagctgctt
      601 tcccgcagag ggctgcgaag aaattccacc cgttccagct tgcactgagc cagcgaagcg
      661 taatccatca tgcagtccgc aaaggtcacg tcgttcatcg ccccctctgc gaacgtcatg
      721 cccgtcatgc ggcagtgttc aaacagtacc cggcgcatgg caaaagcggg gaaaaacagg
      781 tttaccattt cctccaaggg ctgccgctca aagcgccgac cctccacatc gtcctttgtg
      841 cggatcgcct gacggacgat ctcctccaac tccccgtcct cccgcagctt ggccgggatc
      901 acgggcgctt ccggcttcca gtttggctga tgattcatac attctcctgt atacgccgtt
      961 tcttcccggg gcattctagg gccggggagg gatcaaattg cgtcaacttc gttgggtctt
     1021 gtcgggcttc cttttgttgt ccgttctgct ttccgcgtcc gcatgggcgg atgaatatac
     1081 ttcggaggaa cagcggctgc tggaagccta ccattcgggc gaactgctgc ggctgcacat
     1141 tctggcggac aacgacgcac cggaggccca gcagctcaag ctggccgtcc gtgacgcgat
     1201 cctgacctgt tttgccgccg agctgaacga agcctccact gcggacgaag cctttgcccg
     1261 gctgtgttcc ctgcttccgc agatggaagc gctagccgcc gataccgtcc aaagggcggg
     1321 ctttgacctg ccggtctccg ccgaagcggg cgttctgtcc ctgcccgaaa agcgctacgg
     1381 gcaggtggtg ctgccggagg gggactatcg ggggnnnnnn nnnnatggac agatcattct
     1441 gccggcgggc aattaccgcg gccttcggat caccctcggc agcggcgaag gccgcaactg
     1501 gtggtgcatc ctgtttccgc agctctgtct ggctttggcg gatgaggaac catgggtctc
     1561 ccctgcagcc gaaccttctg aattgccgtc tcccgcatcg tccgagcttc agtgggacag
     1621 cctgcgcgtc ctgcgcaact ggctgctcct gccatcagcg ccgtgtgctg cggacgtagt
     1681 tcgcccggaa aacgatcagg gggactaaca ccgccacgcc cagcactgcc gcgccgcgca
     1741 gcccgttcat caaaagcgcc gccacgccca atgcaatgct gaacacgccg ctgaccatgg
     1801 agatcacgcc gtagcggcgg gcgaattttt catccagctt gccggatttc tgatcggccc
     1861 aaaagttgtt gatcaaatca tactggcccc ggaagcggat cattgcgccc atccacaggt
     1921 acaaaatgcc ggtcagccat acgaggatac accagaaaag cacaggtttt cccccttttt
     1981 ccatgtttgt cgttccttgc cgggtttcat tatagcaaat ccggggcaat aagaaaagcc
     2041 ccgcctgtgc ggggctttcg tttattcttc ttccgtaaaa ttttctttcg cttccggcat
     2101 ttcctgacag acgtacagga gcgcatcctc gccgttgtcc tcgtagtagc gcttgcgcac
     2161 gcccagctgg ataaaatgca gcttgcggta cagagcctga gcgatcagat tggtcttgcg
     2221 tacttccagc gtcatgtact gcacgcccag attggcggcg tactgcatca gcgcccgcat
     2281 cagcgcctcg ccaatgccct cgccccggcg gcttttcgtc accgccacgt tggtgatgtg
     2341 cccctcgtcc aaaatgagcc acgccccggc atagcccagc accgcgctgt cttcctccgc
     2401 cacgatgtac cgggcgcagg cgttgcgggt catttcttcc tgaaagctct gcaggctcca
     2461 cggctcgggg aaggtttcca cctcgatggc gtgcaccgcc tcagcgtcgg ccaaggtcat
     2521 gcggcgatag gtcacgctca tgcttttccc tccgccgcca gtctggcggc ccgggcccgt
     2581 tccgcttggg gtgcacgcag ataatgaggc tgaagggtca ggtagtccac ggcgttctcc
     2641 cgattctgcc acgccagcgc cgctacggcg gcgggcctca ggtagctcat ctgcgcaggg
     2701 gcaaacaccg cccgctgtcc caaacgcgct tcgatcgccg tgcggtagcg ctccacaccg
     2761 tcgcccagaa agcagagctt ctgctccggc ttggccgcta caagcgcctg atccagataa
     2821 tcctccagct tggcggcggt atcctccatc acccgctggg gaacccttcc cggctgaaac
     2881 gccgcgccgt atacctgtcc cgcacgtgcg tcctgaatgg ggcaaatcag gtcgggcatc
     2941 aagctcacgc cccacgccag cgcctccagc gcatccaccg ccacgcaggg cttgcccgcg
     3001 ccgtgggcca tacccttcac tgcgctgaca ccgatgcgca cgcccgtgaa ggaaccaggc
     3061 cccacgaccg ccgcgtacag ctcgatctcc gaaatatcgg tggagcactt gttcagcgct
     3121 tcttccacca tgggcatcag actgacggag tgggtcagct tgttcaccgc cgcgccctcg
     3181 tacaggatcc gcccatcttc cagcaccgcc acgccgcaga ccggcccgga ggtgtcgatc
     3241 gccaacagtt tcatccgttc ttcgtctcct tcagcttttc cccgtcgatg gtatgaaagc
     3301 cgcctacggg ccgcagggtg atctcccgtt cgttgtcgtc cgtggggatc agggtgatct
     3361 ccagataggt ctccgggatc acttcttctg cctgactggg ccattccacc acgctgacgc
     3421 cctcgccgta gaggtattcg tccatggaca gctcgtaaag ctcctcggcg ctttccagcc
     3481 ggtaccaatc gaagtgatac aatggcaggc ggccggtttc gtacatctgc atgatgggaa
     3541 aggtggggct cggcagatag ccttcgatcc ccagtccccg ggcgatgccc cgggtcagct
     3601 cgctcttgcc cgcgcccatg tcgcccagca gcaggatcac gtcgccgggc tgcagctgct
     3661 ccgccagccg ggctcccaac gtccgggttt cctcgggaga atgcgtctga tacgtcacgg
     3721 tcaaggctcc tttccaaaag gggagacggc ggggccctgc ccgcgccccg gcaggagaca
     3781 gtacctcctg cacctccgca taattttcta taaaacgatt gaacagcgag acgctgccac
     3841 cagcgtagga agaaacgatt ttggcgctgg cacagtcaaa tcaatacaga ccttgaagca
     3901 ccacgccgcc aagcaagccg tgaaagctat cgtgccgcgg cggccttccc cgcctacccc
     3961 aaacaggcaa cccccccgcc cacatagcgg acggaagcgg gttcaggcct ctagcccagc
     4021 caaaccaact cggccttgga tgccccctcc gccaagcgag ccacggcacc tgtcgtgccg
     4081 cggcggcctt ccccgcctac cc
//
LOCUS       DAWAEA010000090         4078 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3407_length_4078_cov_1.909769, whole genome shotgun sequence.
ACCESSION   DAWAEA010000090 DAWAEA010000000
VERSION     DAWAEA010000090.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3407_length_4078_cov_1.909769"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3470..3479
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          780 a         1187 c         1293 g          808 t
ORIGIN      
        1 atgaactggc gcacgttctg cgcagcaaga acagcggccc ctttgcggtc acactggata
       61 ttctcttcga tgacccggcg gtgtacgaac gggtcaagcg cagcggcaag atcacgccgg
      121 agaacatcgc ccgtctgtac gggctgaagg tggaagatat caccgacttt gccagctatg
      181 accccggtct cggctttaag atcacctacc tgcgcaagac cgcgtcgggc acggtggggg
      241 atcgggacgt gtatggcgcg cagcagcacg cgccgctgat gaacattgaa attcccgacg
      301 agctttgaac cgggactgtg ggagaggagc gtgaacccgt gaagcgggaa gtgctggaga
      361 aaatttacgc gggctttctg ggcatgaacg ccgggattcg gctgggtgcg ccggtggagc
      421 ctgccttctg ggacagcgaa aacattcgca aatattacgg tgagcttcac ggctacgtga
      481 aggattacaa gcattttgcc gcggatgacg acgcgaacgg ccccgtgttt ttccttcggg
      541 cgctggacga tatgccggtc ttcggcgagc ctgcgcctca ggacgtggcc gaggcgtggc
      601 tgaactatgg ccgggagggc gtaggcctgt actggtgggg cggttacggc gtgagcaccg
      661 agcacaccgc ctatctgaac ctgaaaaacg gcatccccgc gccccagagc ggttccattc
      721 atcagaacgg aaagacgctg gcggaacaga tcggcgggca gatttttatt gatacgtggg
      781 gcctgatcgc cccggcagac ccggcccggg cggcacggta tgctgtgacg gcagccagcg
      841 tttctcacga cggcgaaggg cttcatggtg cggcctttat agcggcggct atctcaaagg
      901 ctttcgagac ctccgacatg gaggagatca tcgccgcagg gcttaagcag attccggcgg
      961 actgcctgta ccgcaaggta ttcgacgcgg tggaacggtg ctatcgcaag gatccgcagg
     1021 actggcgggc ctgcctggcc atgctgcagg cggaatgggg ctatgacaag tatcccggcg
     1081 tgtgccacat catccccaac gcgggtgtgt gcgcgctggc gctgtattac ggcaggggcg
     1141 acttcgcccg caccatcgaa ctggcctcca tgtgcggctg ggacacggac tgcaacgcgg
     1201 gcaacgtggg caccattctg ggcgtggccg taggcccgca gggcattgac gagtgctacc
     1261 gcggccccat caacgatgag atcgtgcttt ccggcatttc cggctatctg aacattctgg
     1321 atattcccag ctacagcaaa aagctgttcg gactggcctg ccgtctggcg ggcgaggaat
     1381 gcccggagcc tccggcgcag gatgggacgc tgcactacga cttcaccctg cccggcgcga
     1441 cccatggctt ccatgtgtcc gacccgatcc gctgcatggc gcaggccagc ccggaacatt
     1501 cctttgattc tcagaccctt tcctttacgc tgccggacgt ggagggcggc gtgatcgaag
     1561 aggtgggcct gaagctgacc agcctgtctc aagccaaact gtacgatgcg ggttcgctgt
     1621 acctgacgga tttccgtctg gaaggcaagg gccattgtac gctgaatttg agcctgagca
     1681 aaaaggagtt tgcgtccgtg ctgcccttca gccataacca tggcgcgtgg gagttggtcg
     1741 acggccgcat ggaggtcatg agcctcggcc atgccgaagc catgaccggc ccctattttg
     1801 cccgtgacat gaaggtttcc ggtacggtca ctcctctttc cggcacgagc cacctgatga
     1861 gcgtccgcgt gcagggagcc cagcggggcg tgtacggtgg tttcggcgta gtggacggcg
     1921 ttcgttcgct ggtcatcggt aagaccgttc atggcgtgtg gcagctgctg atctcccggc
     1981 cctttgactg gcaggaagac catgaatacg ccctgacgtt ggcggcggaa ggccagacct
     2041 ttaccctgac gatggacggc ggggagcctc tttccgtccg ggatgatacc gcctgcgcct
     2101 acggcatggt ggggtacgct caatacgcca tgggccgcac ggctttcggc aatttgaccc
     2161 ttgaagaacg ttaaaaaacg gaaaagcgat aaaggagtat atccatgaaa aacagacagg
     2221 aattgaaaca aaaccccgag ctcctcaagg ataagtgccg gggcgcgatc atcggcattg
     2281 ccatcggcga ctccttcggc gacgcttccc gcaagccgga caatcagctg agctacggca
     2341 tcaccacgga tttccacaag ggcgcgtcct ggtccaccga cgatacggag ttcgccctgc
     2401 tgaccgccaa gaccattctg gaatgcaagg gccagctgac cgacgagcac gtggtgaaag
     2461 cgtggctgga aaacgtggcc acgcaggatg aattcaagcg gggcggcgcg tccgaatttg
     2521 aggccagccg caacctgcgg cggggtctgc ggcctccgct gtccggccag tacaacagct
     2581 attgccactc cgacggcgcg gccatgcgca ttgcgcctgt gggcgtgtac tgcgcgggcg
     2641 acattgacgc ggccatcgcc atggcggaaa cggacgcgca tatcagccac tacggcgagg
     2701 gcatctgggg cgcgcaggcg gtcgccgctg ccgtggcctg cgccatggcg gacggaacca
     2761 tcgatgagat tctggcggcg gccatgaagc ccattcccga ggggacctgg ttccgggcgg
     2821 ctatggaaaa ggccttcgcc attgtggatc gggcggaagg caacttcctg aacgcgtgga
     2881 tgcccctgca tgacgaactg tggtgctcct acaaggccac ggtctccgaa gcggtggccg
     2941 aggctttcgg cgtgctgaag ctggtcaacg gagacttccg caccggcgtg gtggccgccg
     3001 gcaacttcgg tcgggacgcg gataccatcg gcgcgatcgt tggctccatt ctgggcgcga
     3061 agcacggcgc gtccactatt cccgcccact gggtggagaa gccccgttat cccaccggca
     3121 cctgcctgac cttcgccaag ggcgtggata tgctggcggt ggccgatgac ctgtgcaaac
     3181 tgattctgga ataaaccaac gattggaaag gagcagccca tgcagaccag cttgaaacag
     3241 cggcccaagt ccaagggaca gcggctcctg atgcggatat ggcaggatcg ggtgctgtac
     3301 ctgctgttgg ctccggccat gctgtatttc gtgtttttcc gcctctggcc catctggaac
     3361 atgcggctgg ccttcttcaa ttacaaggcc cgctccgagt ggacgtgggc ggggctgaag
     3421 tggttcgaga tcattttctc gtccaacgga tttttcacca ttctgggcan nnnnnnnnnt
     3481 acaggtgagc acctatctgc ctcacttcct gtcgtgggtg gttatcgcgg gtatctttat
     3541 caacattctt tcgcccacgg gcggcattct caacgacttt cgggccctgt tcggcctgcc
     3601 tgccgtggac tacatgactc agaaggacac catcatgggc gtgctgttcg cgtcctccct
     3661 gtggcgttcg ctgggctggg actccatcat ctactttgcc gccattatcg gcatcaacca
     3721 gtcgctgtat gaagcggcct atatggacgg cgcgacccgg atgcaggtga tccgctacgt
     3781 ggtgctgccc gcactgatca cccccatggt gaccgccttc atcctgaatc tgggcttctt
     3841 cctggatgca ggcttcgatc aggtgttcaa cttcaccaac gacgcggtca actctaccat
     3901 cgatattctg gatacctaca tctatcgttt gggcctgact cagggtcagt acgaggtctc
     3961 cactgccgtt ggcctgctca agggcgtcgt gggcgtagtg ctggtgctca ttacccacgg
     4021 ggtgtccaag aagctcaccg gcacgggcgt gtggtaagaa aggaaggtgc ggaaaatg
//
LOCUS       DAWAEA010000091         4064 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3417_length_4064_cov_1.976802, whole genome shotgun sequence.
ACCESSION   DAWAEA010000091 DAWAEA010000000
VERSION     DAWAEA010000091.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3417_length_4064_cov_1.976802"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    558..567
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1165..1174
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2917..2926
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          763 a         1348 c         1133 g          790 t
ORIGIN      
        1 ccggtctcgc ccaagatcag gctgatcacc agcgcaggca gcataaacag cgccacaaac
       61 cggagcacaa tggcgatcag ccgggcagtc atgcggtaat tcattatttt tgtcccccgc
      121 caaaaatatc attcagcgcg ttaaagctcc gatcggactt ggtgacgatc accacggtat
      181 catccacggc gaaatagtcg ctgccactgg gaaccagcac cttgtcccgc cggctaatca
      241 gcgccaccag cagattgggt ttcaggggca tgtccttcag cgctacgccc agataccggg
      301 tgctttgggt gacgcgaaat tccagtgcct ccacccgttc atccaccaga cggtgcagcg
      361 tcaaaacgcc ctcgccctgt cggttctgca tggcgcgcac ataacggaga atgttggtgc
      421 tggtcagctg caggggcgtg accacacact cggcctcgtt ggcgggcagc agggcgcagt
      481 attcgccccg gttgatcttg gtgaccacct tgggcacccg gcgggaattg gcgtacatgg
      541 cgatgaccat gttctgcnnn nnnnnnnggt gttgatggac gtgccgtcgg cgttgatgac
      601 ctctgtcttg ggcagatcgg cgctaagctc gtcgcagcga tccggctttg tctccagcag
      661 cttgacgtga atgccagcgt ccgccagcat ccgggccaga taaacggccg tccggctgcc
      721 gccgatcagc atgaccgagc ggatcttctg ggtctccatg cccatccact tcaccatctg
      781 caagagggac tgcaccgtgg aggtgacata gatatgatcc ccgacctcca gacggaagga
      841 gccgtcggga atatagactt ccttgttgcg ctccactgcg cagaccagta cccgcacctt
      901 caaagaggtg tacaggtcac tgagaaccat gccgtccagc ttgccgcccg cgcgcaccgg
      961 cagcgccacg atctcggcgc ggcccttggc gaaaggctcc cgtttcagga aagacgggta
     1021 ttgcagcaga ccgaaaattt ccttggcggt ggtgcgttcc gggttcaccg tcatacttaa
     1081 tcccagttct tcccgcaaaa gataggtttc ctcggcgtaa accgggttgc ggatgcgggc
     1141 gatggtgtgg gggcagccca gcttnnnnnn nnnngcacag caggttggtc tcatccgcac
     1201 tggtcacggc gatcatcagg tcgcacttgc ccacctcggc ggcctgctgc accttgacgc
     1261 tggcaccgtt tccttccagc accatcacgt ccagccgctc ctccacctgc accagcgcat
     1321 ccttgcggtt gtcgattacc agcaggtcat gcccttcccg gctcaattcc cgggtcaggg
     1381 taaagccgac cttgccctcg ccaatcacta cgattctcat agtctactcc ttcgcaaata
     1441 accccatcgt aagtctgcat gaagcagcaa tcatcatagc actttgcccg caagattgca
     1501 acgggcgcga aaggttcccc ctccgcgccc atcgattctt tgcagttcgt caacgaaagg
     1561 ttccgctttt ggggttcagc agcgcataca cataggtgtc cttccactgg ggaagaccct
     1621 gttcgtccgt ccagaaatac acgttttgac gaagatgcgc ttcccgcgcc atgcccagcc
     1681 gttccagcag ccgccatgac gcttcgttcc ggggatcgca ctccgcgtag atgcgatgcg
     1741 tgccctgccg gaaggcttcg ccgatcagag cctgacagcc ttccctggca tagcccctgc
     1801 cccaatgttc gcgggccagt acatacccca gctccagcga ctgaaactcc cggcggccca
     1861 gatagagatt gccgatgtaa actccgtctg tcttccgttc catcgcccaa aattccccgt
     1921 gatccagccg agaagccaac gtttgccggg cttcctcccg gctcatggga cggtaaggct
     1981 cgtagcggac gacttccggg tcggaaacac aggcatagaa gccgtccaga tcgtcctccc
     2041 ggtaggggcg aagaatcagc cgctccgttt ccagccgggt catgcccgtt cccccgccgc
     2101 ttcttccgcc agcttggctg cgccccacag cgcgtcatgt gcgggcggaa cgacctgcgc
     2161 cacggtctgt aaagcctcct cggccagcgt gcggtaaatg ccctcgtgat ccagcaggcc
     2221 gcccagcaac gccagccgag gcttctccat attcaggcgg cgggccagcg cctccacaat
     2281 tcctcgaagt tcctgtgcgc cccggcgaag gatagccaat gcggcttcgt cgccctgctc
     2341 ggcacattcc accgtgaccc ggctcagcgc ggcgactcct gccttgcccc ggttctggta
     2401 gtatacatag ttcaggatat ccccggtgtt cttgccgcca atgaaatgca gcacgctgtc
     2461 tcgcaaaagc gccgcgtcca tccgtccatc cagtgcccgc acagcggcgg acagcgcgtc
     2521 ccggcccagc gcatagccgc tgccctcatc gtcgatcaga tggccgaagc cacctacccg
     2581 ggccgtttca cccgcttcgt tcaccccgaa gccgatggag cccgtgcctg cgatcaggat
     2641 gcagccgggg ccgtccaccg cgccccgcag ggcgatctcg gaatcgctgc gcagcagcag
     2701 cttccccgca aatccggtcg gtttcagttc ctcccggatg atctgttcca gttcgccgca
     2761 ggaaacgccc gcaccgccca cgcagatggc gcggcattcc tccagactgc cccagtcggc
     2821 gaacatctcc cgcagcagtt tccgaaaggc ctcctcgccg atggcggaaa tattgaaagg
     2881 cccgtatttg cggcgttcgc tctgcctgtc ccggtgnnnn nnnnnncgat aaacatccgt
     2941 tattccctac tttcattggt ggtttgctgc ctacgcgcag cttgacaatc ccgattctcc
     3001 gggctacact gaatgcaaaa tcatccttcc caaaggagga tcttccatgg aacatccgat
     3061 tctgaagctt cttcaaaagc cgtcccgcat cgtgctgggc ttcatgtccg gcaccagcgc
     3121 ggacggcgtg gacgcggccc tctgccgcat cacgggctgc ggcctgacca cgcaggtgga
     3181 gcagctttcc tttaccttcg tgcccttccc gcagaaagtc cgagacgaga tcctccgcct
     3241 tgccagcggt gagaccgcct atgcggcgga cttctgccgc atcaactttc tgctggggca
     3301 gctgtactgt gaggcgcagg tagacctgat cggcaaccac ggccagacgc tctggcacat
     3361 ccccgtggag gaggaatatc tgggccgtcg ccagcgcagc accttacaac tgggcgaaga
     3421 cgcgctgctg gcagaatgct tcggctgccc ggtggtgggc gattttcgcg tccgggacat
     3481 ggcggcgggc ggattgggcg cgccgctggt gccttacacc gaattcctgc tgtaccgccg
     3541 tccagacgaa tgggtagccc tgcaaaacat cggcggcatc ggcaacgtga ccgtacttcc
     3601 ggcgaactgt acgctggatc aggtctttgc ctttgatacc ggccccggca acatggtcat
     3661 cgacgcggtg atctcccggc tgacgaacgg ccgcatgacc tacgacgacg gaggagccat
     3721 ggcggcgcag ggaaagcttc accccgaact gctgcgctgg atgatggacg acccctatct
     3781 ttcgaaaaag ccgcccaaga ccacgggccg ggagctatac ggccccgttt acatagaccg
     3841 gctgatggaa aaagccggga cgctgaacgt cgccccggcc gatctgatga acaccgtcac
     3901 ccaatttacc gccgagacca ttgcctacgg gctgcgccgg ttcagtcctc aactgcccca
     3961 aaggctgatc gtgggcggcg gtggcagtct gaaccgctgc ctgatggagc agcttcgtca
     4021 ggtgctgccg gaatgccgcg tcatgaccaa cgaggatctg gggc
//
LOCUS       DAWAEA010000092         4057 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3422_length_4057_cov_1.823088, whole genome shotgun sequence.
ACCESSION   DAWAEA010000092 DAWAEA010000000
VERSION     DAWAEA010000092.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3422_length_4057_cov_1.823088"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3149..3158
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3599..3749
                     /estimated_length=151
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          755 a         1098 c         1280 g          763 t
ORIGIN      
        1 gaagtgggtc cagggcatag ccctgggagg aggttggatg gagaagaagg agatcgtccg
       61 tttttcccat gtgacgaaac aatacccggg aacgctggcg aacgacgacg tcagcctgag
      121 cgttcggcgg ggcgaagtgt tcgcactggt gggggaaaac ggcgcaggaa aaagtacctt
      181 gatgaacttg ctgtacggaa tgcagccggt caccatgggc gaaattttca ttgacgggga
      241 aaagatcggg ccgcagcatt gcccggaaaa ggccatggag atgggcgtga gcatggtgca
      301 tcagcacttc aagctggtgc ccagctttac cgtggcccaa aatatcctgc tggggcatga
      361 accgaaaaag ggattgagct atgattttca aacggcgcgg gacgctgtgc ggaaactgtc
      421 cgagacctac ggcctgtatg tgaacccgga cgatgtgacc ggcgaactga gcgtgggcct
      481 gcagcagcgg gtggagatcc tcaaggccct gcgcaccggc gcaaaggtgc tgattctgga
      541 cgaacctacc gccgtgctga ccccccagga gacggacgag ctgttccgcg tcattcggca
      601 tatcgtggaa gaacagggca tgacggtgat cctgatcacc caccggctgc cggaggtcat
      661 gcaggtcagc gaccgagtgg gcgtgatgaa gcgcggccgt ctggtgcagg taatggagac
      721 cgctcagacc agcgaacggg agatcgcctc cctgatggtg ggacgggatg taatcttcga
      781 cgagctgcgc cagcagcatg aaatgggcga ggaaaagctc cgggtggagg gcctgtgcgc
      841 cctgaacgat cgaatgctgc ccgcactgcg acaggtgagt ctgtccgtcc gcgcagggga
      901 aatcgtgggc gtgtgcggcg tggagggcaa cggccagacc gagctggctg aatgcattat
      961 gggcatgcgt ccccagaccg gcggacgggt gctgctttgc ggcgaggacg tgagccggaa
     1021 aacgccgggc gagattcgga gccggggcgt gagcttcatc ccggaggacc gcatgactac
     1081 cggcgtggac ggaaaggcca gcgtggcgga aaacctgctg gtgggcaagc agcgtcggcc
     1141 ggagttcagt ctattgggca gtctgcacct gcgccggcac cgggtgaagc agtatgccgc
     1201 cgaacaggcg gaaaagttcg atattcgcat ggccagcgtg gacgacccgg tatcctcgct
     1261 gtccggcggc aacatgcaga aggtggtcat cgcccgggaa ttcagctttg attccccggt
     1321 gctggtcatc agccagccca cccgcggcgt ggacatcggc gcgatcgagt tcattcacga
     1381 gcgcatcatg cagaagcgca acgagggctg cgccattctg ctcatcagcg ccgatctgga
     1441 cgagctgttc cgtctgagcg accgtctggt gacgctgtac gaagggcaga tcaccggctc
     1501 tttccagacg gaggcaatca cccgggagga gatcggctat tacatgaccg gcggcgggaa
     1561 ggacgaggaa agcgcggcaa aggaggaatt gcggcatggg taacggcttt ttccagagtg
     1621 ttcgggcgct gcttcgcctg aaaccggcgc agaagctgaa ccgggactac ctgatctccc
     1681 tgctgtgcgc ggtgctgctg gcctttctgg tgggggctgg catcatgctt ctgtgcggct
     1741 tcgaccccat ccagtgctac ggcgcactgt ttgaaggagc gctgggcaaa ccccgggcca
     1801 tcggcaatac gctggccaag gctacgacgc tggcgctgtg cggccttgcc atgagcgtgg
     1861 cggccaaagc ggggattttc aacgtgggcg gcgaaggaca gctctttatg ggcgcactgg
     1921 ccgccgccgt ggtgggcgcg caggtaaagg gcgtgcccgc gtgggtcgtg gtcattctgg
     1981 cgcttttggc gtccctgctg gcgggcgggc tgtacgcatg ggttcccggc gcgatgaagg
     2041 tctactggaa ggtggacgag gtcatcacaa ccatcatgct caatagcgtg gccatcttct
     2101 tttgcagcta catggccaac ggccccctga agaccacgga gaagggcatc gcctccggca
     2161 cggccagcat tatgaaggaa gcgggctttg ccaagctgat ccccctgagc aacctgacca
     2221 cggccatcct gtttgcggcg gtggcggcat ttttcacgtg gtacctgatg acccgttcct
     2281 ccgtgggctt cgaaatgaag ctgaccggcg ataacgaccg ctttgcggcc tacggcggca
     2341 tttccgccaa aaagctgatg ctctggtcca tggtgctgtc cggcgcgatc tgcggcctga
     2401 cgggtatgct ggaggtgttc ggcctgcaca agcgcttcat gaccactatt tccaacgagt
     2461 tctactttga cggaatgctg gtggccatga tcatgcgcta tcagccattc ggcgtgattt
     2521 tgatgagcct gttctttgcg gtgctgaata tcggcagccg ctccatggag ctgtccgccg
     2581 gaatttccag cgaggtcatt ctgattgtgc agtccatcat catctttttc atggcggcgg
     2641 agggcggcat ccgcacggcg ctgcagaacc gctccgccat ccggaaggcc cggcgggaag
     2701 ccaaggcgaa ggaggcgggc gtatgagcga aattttcaat gtgcttctgc tgcagaacac
     2761 cctgcgcacc gccacgccgg tggtgctggc ggcgctgggc tgcctgatga cccagcatgt
     2821 gaacatcacc aacatcggta tcgacggcat gatgctcatc ggcgcgtttt tcgccgtgct
     2881 gggcagctac tactgcggca gctgggcggc gggactggcc tttgccatcg tgttcggcgt
     2941 gattctgggg ctgctgtact acgtgctggt gattcgcttc cacagcgatg aatttatcat
     3001 cggcgtggcg ctgaacatct tcgcgggcgg actgaccact ttcctgctgc gcacgatctt
     3061 tcaggtgaag ggctccttct ctgatccgcg catcttacct ttgcccacca tcaccattcc
     3121 gggagtaaag gacatccccg tgctagggnn nnnnnnnncc cgtggggatt ccacctgcgg
     3181 gccagcggcg aatatcccga cgcgctggaa gccagcggcc gaagccccga aaagatgaaa
     3241 ctcagcgcca gcgtgctctg cggcgtactg agctgcatgg cgggcgcaca cctgtcgctg
     3301 ggctacctga ccatgttctc cgagaatatg tcggcctccc gcgggtatgt ggccttcgcc
     3361 tgcgtgatct tcggccgggg caatccgccc atcgtatttc tggcggcgct gctgttcggt
     3421 tttatcgaag cgctgggtct gcggctgcag aagtacgtcc ccagcgacct gaccggcatg
     3481 gccccctatc tggtcacggt cattatgatg gtggctgtgg tgctgtggga gcgaaaaaag
     3541 aaaaacaaag cggtcaaagc agaaggataa ggaaaaagcc ctcgctgcgg tctggaaann
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt tccagccggg agctgttcca ctgcagggcc
     3781 agctcccgct tttctccgtc ccagacgaag gactgatctt cgcttaacgc cgggaaaacg
     3841 gtcccctctc ctgtggtcag gacacagcgt tcgacctgct cggtggcata ctgcacttcc
     3901 agaaccagct ggttcgcttc gtgttcccga accgtgtagt cgcgccatgg ggcgggtgtt
     3961 tcagccagcg ccgaagaacc gcacagccag acgaacagca gcaatagaaa acgttggatg
     4021 attcgaggca tgaccttccc tcctgtcgtt gatgaag
//
LOCUS       DAWAEA010000093         4046 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3432_length_4046_cov_2.817840, whole genome shotgun sequence.
ACCESSION   DAWAEA010000093 DAWAEA010000000
VERSION     DAWAEA010000093.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3432_length_4046_cov_2.817840"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    271..322
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2283..2292
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3372..3381
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          754 a         1169 c         1341 g          710 t
ORIGIN      
        1 gagaacagct ggctgcaatg gctgcggcct tacgatccca acgcggaatg ggtggcttat
       61 tgcacctccg cgctgacggc agacggcgtg gtgcagggcg tgctgctgta ttcctccccg
      121 gtgcaggaaa tgatggaccg cctgtcctcc ctgcagaccc ggatggtggc gtacttcctc
      181 atcgcggcgg cggcggccat ggtgatcgcg ctgatcttct cccgagtgct gacccggcct
      241 atcgtgtccc tgaccaagag catccagcgc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nngttcaata ccatgtccga aaagctgcag atgctggata
      361 ccagccgcaa tcagttcgtc tccaacgcca gccacgagct gaaaacgccg ctggccacca
      421 tgaagatcct gctggaaaac atcatctatc aaccggaaat ggacgcggag atgcgtaccg
      481 agttcctgac cgatatcaat aaggagatcg accggctgaa cagcatcatc agcgaccttc
      541 tgacgctggt gagcatggac tcgaaggcca tgcgcctgaa ccgcaccacc ttcagtctgg
      601 cgcaggtgat caccgatacg gctcatcgtc tccagcccgt ggccgagagc catcatcagg
      661 aattgaagct ggccttgaac gaccgctgcg aaatgtacgc cgactgcgcc aagctgacgc
      721 aggtggtgta caatctgatg gaaaacgcca tcaagtacac gcaggagggc ggcaccatca
      781 aggtgcggct gatccgctcc ggccgggacg cggtgatgac cgtcaccgac aacggccccg
      841 gcattccccg ggaggatcag gctcatatct tcgaccgctt ctaccgggtg gacaaggccc
      901 gcagccggga gaccggcggc acgggtctgg gcctgagcat cgtgcaccag atggtgctga
      961 tgcacggcgg cagcgtgtcc gtggaaagcg aagaaggcag gggttccact tttaccgtgg
     1021 aactgcccat ccatcaggga tgagccaagg agagacgttt atgaaaggaa atcgaaagcg
     1081 ccggggaatg ctggcggttc ttctggcggc gctgtgcggg ctgctgtgct cctgcacccg
     1141 ggcagcgaat ccgctggtac aggccgaggc cacgccgctg ccaggcgtgg agcagcgcct
     1201 tcacgccgcc accgccgcgg aggacaacgc cgcttcgtgg gatgtggtgc tgtatttccg
     1261 ctatctggat tattccatgc tggcggggga aagccgcacg ctgaccgtcc gaaaggacga
     1321 aagcagcgaa gcggcgcttg tgcgtgcgct gctggccggg ccgtccgccg gggagatgaa
     1381 tcttcgtcgg gcgctgggag aggacgtagg gctgaaggaa gtcaccgcca gcggtgatat
     1441 gctgttcatc accctaacca attccctgct gctggacggc attccagacg gctggcgctc
     1501 ggacgagttc tgggcggagg aagcccctgt ccgccgccgg ctggccatgg ccagcattgc
     1561 ggacacggtg atggagaatc tgccctacac ccgggtgcag attttgatcg acgggggaga
     1621 gagcgccgcc atccggctgg atcggagtta ttacctgacc ggggaacagg gccccgccga
     1681 cccgctgagc cgggacgaaa gcctgctgct gagcccgtac aacaccgccg ccctgctgat
     1741 ggaagcatgg cgaagccggg attttgatac gatctacgat ttcaccgtgg cggaggacct
     1801 gccggtgcca gagcagttcc gggcggagat cgacgcggcc cccagcctgt cggactacac
     1861 gctgaccgcc gggatggtca acggcgaacg tgccgccctg aatgtagacg cttccctgct
     1921 gacggacggc gcgtccaccg ccctggaggc atggccgctg acgctggaaa agcagggcgg
     1981 cgtgtggaaa attccgctgg agaccctgcg gcggctgatg gggctgtaag gaaaaaagat
     2041 agcgcaaacc gccaatccca ccgaaaaaaa ggagagagga ctgccgtgaa agggaagaaa
     2101 cgcgcgttgg cgctgctgct ggcggcgctg ttgccggggc tgtgcgcctg tcaggccggg
     2161 agcagtctgc ccgcgtccga ggcgtccccg gagatcacct tgcctccggc ggttcagagc
     2221 tatgtggcgc ccatcggtga cgcggcgctg gaatacaccg ccgacgctac gctgtacctg
     2281 ccnnnnnnnn nngccgtggt cagcccggtg acgttttccg catcccggcc ccgggtggaa
     2341 agtctggtgc gggcgctgct gaaccactcg ggcacgtcca ctgccgccgc gctgggcggg
     2401 aatgtaaagc tggcgctgta cggggccaac ccggtggagg tcagccggaa cgcggccacg
     2461 gtgaatctgg cggcttcggc gctgcaactg aaccggggcg acctgtacat tgcttttcag
     2521 gccatcgcca acacgctgac ggagctgaag gagatcgact acgtgaactt cctgatcgtc
     2581 gatcatcccg tagggctgga cgtggccaac accctgccca tggggtcgtt cagccgcagc
     2641 ctgagcgagg atctgtcctc ggtctacgaa cagcggctca gccgccgggt ggggctgaac
     2701 gaaagcgcgg aggacaagtc cctttccgcc aacgtgacgc tgtattaccc gctggcgggc
     2761 gtgccgggac tgcttagcga ggtgcgctct ctgagctttg ccaaccagaa gccgtccgat
     2821 atgatcgtcc agatcatgcg ggaattgagt aagggcgcgt cggtttcgga gatcgactcg
     2881 ccgccgctga atctgctggt ggagatgctc accggcgacc cggaggtcgc ctattccgac
     2941 gagctgggcg gcagcgtcat tacgctgaat ttctcctatt ctttgaacga catgctggaa
     3001 aaggcgaacg tgagccgggc caactgcatg gcttcgctgt gctacacgct gtgcagcttt
     3061 ttccccgccg cgtcgggcgt gacggtgacc attggcgagc agccggtgga gacactgatg
     3121 ctgacggaca gctttaccag ctccatcgtc tttaccgatc aggtgatgaa gcgggcggat
     3181 tttgccccgc tgctgtgcga cctgtgcacc ctgtactttg ccgacggcga gggccgaggc
     3241 ctacaggcca gcaggcggcc ggtggtctat tatcagaagc agaatccccg tgcgctgctg
     3301 ttggagctga gcaagggccc gcagccctat gacgaggttc ccgggctggc ggcggtgatg
     3361 ccggtgggca gnnnnnnnnn ncctgtcaga cgcggacatt ctggggctgg cgctttccgg
     3421 ccggacggtt ttggtgaatt tcgccccggc atttctggtg gcggggcagg gcatggacgg
     3481 ccagcaggag cggctatttg tctacgccat gacgaacacg ctctgcgaga accagacgat
     3541 cggcagcgcg gcgtactttg tctccggggc ggtgccggag gggttctccg gggagatcta
     3601 ctggggcggg gagttttatc ccctgaaata aatgggaaag cggaagcggc gcggagtgtg
     3661 cggcttccgc ttttttgtgc gggcattcag gcgcggggat atttttcggc ggggcctgac
     3721 gcgccgactc cggcgcggga gtgggacttg ccgtcccata ggcgttattt tcagaggacg
     3781 aaccgaaggg gtgcaggggg cgagcggaaa gccccctgca agaggcatta catgacaact
     3841 ctgccggaag cagcttcctg ccgggggcat gacgcgccga ctccggcgcg gatgttgggg
     3901 ctgccgcccc gaggggggca gtactttctt tcgaagcgaa agaaagtacc aaagaaagca
     3961 cgcggcacgg cgactacggg aaaaaggctt ctattgccca ttttgacggc ggggctcgca
     4021 atgtcgcgcg caatggagtt ggatag
//
LOCUS       DAWAEA010000094         4035 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3446_length_4035_cov_1.932663, whole genome shotgun sequence.
ACCESSION   DAWAEA010000094 DAWAEA010000000
VERSION     DAWAEA010000094.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3446_length_4035_cov_1.932663"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3783..3798
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          782 a         1246 c         1232 g          759 t
ORIGIN      
        1 ggttcgtcgc cggggtgaat atcctcaaat tcccggtttt cctccaacac ggacaattcg
       61 ccgcacatgc tcttcatcag ctccttgtag ccggacagct tcagggcctt gcaaagccgc
      121 atgacggagg aaacgctgct gccgcaggcc tgcgccagct cctccagcga catgccggag
      181 gcctgacggg ggtgctgcgt caggtaatcg gcgatctgct gctctttggg gcttaatgat
      241 gcgtaggatt ctttgatgcg gaaaaagcat cctccggctg cggccatggc tatcctttcc
      301 ttgtgtccag tataaaggaa agttttcctt ctgtcaaatg aaaaaagaaa agttctatgc
      361 aaaaattgaa aaacagcatt gacaattact tgtcatactt ctataatgaa gctggctcta
      421 aaggagatgt ttccctttcg ataaaaaaac aggtttttga aggagggttc ttgttatgat
      481 cgatctgaac cgccgtcccc ggatgggact tttgctgatc tcccccgaac gcttccagac
      541 cattggcgag ggcacggccc gcggcagcta caaggaacgc aagcagaagg aagccgaatg
      601 gatggccaag gaagcgtccg ctatcgcgga agtgaccttc cccggcgtga tctacaccgc
      661 cgaggatgtg aagcgcgcca tcgacgcttt cgtgaccgcc aaggtggact acgtgctggc
      721 tatttacctc agctgggcgg aggacttcgc gtggattcgc ttcctgcggg atatgcccgc
      781 ctgcccggtg ctgttctgcc atcggatgcg ggacaagatc gaactgaagg acacccatga
      841 tgacgacgag ttcgccgaat atctctgctg cggcggtttg gtaggctcgc tggaagccag
      901 cggctccgtc aagcgcttca accgccccat gctggaggtc tacgccggta cctggacgga
      961 agttctggcc cgcgctaaga ccttcggaaa cgccgcccgg gcccgcactc tcttccggga
     1021 aagcaccatg ggtctgctgg cctgcatgaa cgaggtcatg tggagcacct acgtggatcc
     1081 ctacgccatc ttccgggatg tggggccgga gctgcggttc ctgtccgtgg cggagctgga
     1141 agacaccgtc aacaccgttt ccgacgcgga cgccaccgct gtgatgaagc gtatcgcgtc
     1201 ccagtatgag gtgctgccca acgtggacga gggcaagttc ctagcctccg tccgggcctc
     1261 catgggcatg gagcgtctgg cggaaaagaa ggagctggac ctgctggtgc tcaacgatat
     1321 cgacactgtg ctgttccagc atatcggtct gcgccccggc ttctggccca cctcgcccga
     1381 ggtgaagacc cttatcgtgc ccgaaggcga catcggcggc cgcatcgcct gcttcgcgct
     1441 gaagctgctg accggcggcc atgtgaacta tatcgagcct ttccacatcg acctgcccaa
     1501 ccacggcttc gacgcgggtc acgcgggccc caacgattac accgatcccc ggggcaagtg
     1561 caaaatttcg tccgacgtgc gttttgccaa gaccaagtgg aaatacgccg gcgcgccctt
     1621 cgcgtggtac gtgttcccgg aaggcgaaaa gaccatgctg cactgctctc agcagacggg
     1681 caagttccag tttgtggccg cccagatcga ggccgtgcat accgaccact tcctggcgac
     1741 ctattcccac agccatttcc gccccgtggg cgaaagctgc accgagctgt tcaccaagct
     1801 gctccggcag ggcgtgaccc agcactacgg catcgtggac ggcaacgtgc aggctgagct
     1861 gcaggatctg gccatgatga tgaatttcga atttgagcgc gtgtaagtgc tgaaaaagaa
     1921 cgacccgagg aatcaaaaag gagagacgaa catggaacgg atgaagaaaa tcggaaccct
     1981 tccccttcgc agcgctgccc aggtgggccc ctcccgtatc ggcatcggct ttgaaaagct
     2041 ggatcgcaag gtgttcgagc cggaaaaggc ctacgatctg gtagccgcta ccggcgtaca
     2101 ctacgtgcgg ctccagtccg gctggcagcg cacggaaacc gaaaaaggta aatacgactt
     2161 cgcgtggctg gatgatatcg tggatcagat catcgcccgg ggcatggagc cgtggatcga
     2221 cctgtgctac ggcaacgagc tttacactcc catggcgaag gaatacttcg gcgcggtggg
     2281 ctgcgcgccc atcgctaccg aggaggagcg ggagggctgg cggcgctacg tcaaggccgt
     2341 cgtgacccgc taccgcggca aggtgaagtg gtacgagatc tggaacgagc cggacggcca
     2401 gtggtgctgg aaaaccggcg taagccccga ggaatacggc gatttcgcgc tggccacggc
     2461 tcgggccatc cacgaggccg acccggaggc caaggccatc gcgggcgtgc tgtgctatgt
     2521 gaatctgccc tattgcaagc ggatgctgga ccggggcgtg gcggcggagg ccgacgcggt
     2581 ctccttccac cgctataacg ccaacgagct ggacgcgctg aacgaaatgc aggccctgcg
     2641 ggccctgctc gaccgctaca atcccaaggt gcagatcatt cagggcgaaa gcggtaccca
     2701 gtccgattcc cggggcgcgg gcgcgctgcg gggcggcgcg tggacgcccc ttaagcaggc
     2761 caagtacctg ctgcgccacc ggctgatcga tctgtccacc gatatggtgt tcacctctca
     2821 tttctccgcc atggatatga tcgaagccct gaatggcaaa gtcggcgaca aggcctccta
     2881 tctggacttc ggctatttcg gcgtcattca ggccgatttc aacgaggagg ggctggccac
     2941 cggcgagtac acgcccaagc tgtcctaccg ggcgctgcgg cacctgtgca ccctgtttga
     3001 cggaaaggcg cagccggagc agctgcccgt ccgccgggtg gtgaatccct ccgcccgggt
     3061 gtttgataag gacgcttccg acagccgtct ggtaacgctg ggcttccgcc ggggaaacgg
     3121 cacggcgctg gcctactggg aagccgccga cctgatgcgg gagacctacg acggcaccgt
     3181 cagcttccag ctggctggcg tgaaggacgc gccccgtctg gtcgacctga tgaccggcga
     3241 cgtttatcag gatgtaggcg gcagctttct gaaatacggc gttgccgatg aaaagggcgc
     3301 gctgcttccc ggcagcgtgg gcgaagcgcc cgcccgagcg gacgaatcgc cggagaaggt
     3361 ctacgaggct tttgaggaag tcatccgccg ggcgcgggag actgcgccgc tggccggagc
     3421 ctgcgcctgc ttccccggcc cctttgacta cacccacggc gttttccgga tgaagcacaa
     3481 gtttcaggcc ctcttcggct gctccatcct gccgcccttt caaaaggcgg gtctgcccgt
     3541 ccgcttcctg cacgactcca cctcctacat tctgggcgag gccttcgacg gaacgcttca
     3601 gggagccgaa agcccctgct gcgtgatgct gggcaccggc cttggctaca ccatgatgaa
     3661 ggacggcaag gttctgatcg atgaaagcca gaccccggcc ctttccctgt ggtgcacgcc
     3721 cttccgggac gggatcgccg aggattacgt atccacccgg gccattcagg ccgcctatgg
     3781 ccnnnnnnnn nnnnnnnngc gaagccctgt ccgggctgat ggggatcatc gaccggcgct
     3841 tccatccgga caagctggcg ctgggcggcc agattgcccg ctccgccgac ctgctccatc
     3901 tgagccttcc cttcccgtgg gaggtctctc accacctgga tgacgccgct ctgcggggcg
     3961 cggcctacta cgccctgcat tcccgggagg actgcaccgc cgtgctgccc aaaatccagc
     4021 tctgaccatc ccgct
//
LOCUS       DAWAEA010000095         4009 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3472_length_4009_cov_2.690693, whole genome shotgun sequence.
ACCESSION   DAWAEA010000095 DAWAEA010000000
VERSION     DAWAEA010000095.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3472_length_4009_cov_2.690693"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          686 a         1232 c         1281 g          810 t
ORIGIN      
        1 tttggcacgg cagtgccaaa gacgacgatt gaggttgcgg atcactgccc ctgccctgct
       61 tagatgacgt tgtgctcttt gaggagcttt tcgatgtcgg tgccggcgat cttgcccata
      121 agagccttga aggccagctt ttccaagagg cccgcgtcca tgtccgtgac cttcttgccg
      181 tcccggagct tcacggtggc gttgagcagg tcgcgcacgt cgtagacgct gccccagacc
      241 tcgggcacgc cccgatccac gttcagcagg gtgtagacgg cttccatacc ggtgcgcatg
      301 gagtactcgg tagtgaagat ggtgtcgcgg ggcgtttcgg cgaactggcc caggaaggcg
      361 aagttcacag cgccgtccgg cacgaccttc gggcggtcgc cgtaggcccg gggcatgaag
      421 aacgcgtcga tgtagggcat catgacgggc acggtgttgg cgctgtgctc ggccatgtct
      481 tcgatctcat ccaccggcac gccgatgtga tacagccact ccatgcagat ctccttaccg
      541 gtgcattccc gcatgggctt tttgacatag tcgccgggct tatcggtgaa caggctgtac
      601 acccatacca ggcagtgatc cttgggctgc tcccggaact ggggctgacg gttgatggtc
      661 cagctcatga gccagctgga atccttcacg gtgacgatgc cgccggtgac gaccttgccg
      721 gtgaagggat cgcgcttgca gatgttcttg atgtagggga tgatgcgctg atctagcgtt
      781 tccacggtgg cgctcatcca gttggtctgc tcggggtcgc tgcagaactt gtccggatgg
      841 ccgaaggaag gatcctgcgc ggcgatcttg cgccacatat cccagccgcc gccgggcttg
      901 agctccgtgt tgtaggctgc gggctcgttc tggctgccca tggaggagtt ttccacacag
      961 ccgccgttgg tgatgaacac cagatcgttc tccgtcaggg cgatggtatc ttccttgccg
     1021 tcccgcagaa cttcgatggc ggtagccacc ttgtgatccg gcttgctgca gtcgaacttc
     1081 acgttgacca cctgcacgcc gaagtggaat tgtacgcccg ccttttccag atacttcatc
     1141 atgggcagga tcatgctctc gtactggttg taacgggtga agcgcagggc tttgaagtcc
     1201 ggcaggccgc cgatgtggtg gatgtagcgc tgaaggtagc gcttcatttc cagcgcactg
     1261 tgccagttct caaaggcgaa catggtgcgc cagtacagcc agaagttgga gttcagcacc
     1321 tcgtcatcga agaagtcggt gatcttcttg tcctgcaggt cctcgttggg ggtgaagaac
     1381 agcttcatga tctccatggc gcccttgtcg gagatgtcga acttgccgtc ggtgtgcgcg
     1441 tcctcgcccc ggttgaccgt ggcgcggcac agagagtagt tggggtcgtc cttgttgagc
     1501 cagtagtact catccagcac gctgacgcct tccgtctcaa tggagggaat ggagtggaac
     1561 agatcccaca tgacttcgaa atggttgtcc atttcacggc cgccgcgcat cacgtagccg
     1621 atattctcat acttccagcc gtcgcacgcg ccgccggcgg tggggccttt ttccagaacg
     1681 tggatgtggt cgcccttcat ctggccgtcg cggaccagat agcaggccgc cgtcagggcc
     1741 gcaaggccgc tgcccacgat ataggcggac ttttgatcca cgccttcggg cttgcgggga
     1801 cgggcaaagg cttcgtaatt gccgctggaa taatacatgc tgaaatcctc ctcattttga
     1861 ccgggttggt tcaggaaata ttcatcttcc gccgggaggg agctttcgtc gccccgcttc
     1921 ccttgcggtg attgtaggat agtcttttcc caaaaagaaa acaataaaca caaaagccgc
     1981 cgtgatgaaa ttttcgtcac ggcggctcat gtgtcagaat tttgggcacg aagcttgatt
     2041 tgatgaaatt ttgttcacgg aggattccgt gtctgatttt tattcccggc gcagcaccac
     2101 cacggccagt tccgtggggg gcagggcaaa ggtgtgcccc tccccggcgg cgctcagggg
     2161 caggggggct tccgtctcag gcgcatcctc cgtcaggacg acggcgcgcc acgttccctc
     2221 gggcagggta acggtcacgc tgccctccag accgccctcc cgggcgcagg cgatcagcac
     2281 gccgccgtcc ggcaagtcat accggcgggc catcaccgtg ccgcagctgg cgctcaggcc
     2341 cagcgtatcc cggaaaaggc cccgcccgta gcgggtcagc cacgcccggc gcagatcatc
     2401 ggtcttcctc acccggggcg tctggggatc atcgggctga tagcagttgt cctccatcag
     2461 gtcgtaatgc cagaggtaca taccgtccac aaaggcgcgg tagagcagct tgcaggcccg
     2521 gcgggccata tgctccggac gcatgccgga atggcggcgg ggattcatca tgtcgatcag
     2581 tccctggcgg gggaaggtgt aacggtacag ctccgggaaa gccccggcgt gatcatagaa
     2641 catagtggta atcagaccgc cgcagatccc gggcatgtag gtgtcgttgg cgccctccac
     2701 cagcagggcc acgccttttt ccggggcttc ctgggccagc cgggtcagca ggcggcgcag
     2761 gccggtgttc cagccgtccc gcacgccggg atgatcgtcg tgggcttcgg agaagcacag
     2821 gcaggaggta gccatggcca gctgatccag atacaggctg tccgcgccga tgtcatgggt
     2881 cagccagtgg aacacgctga gcagctcgct ctgccacagc tcggcctgac tgcacatgga
     2941 ggcgaaatac tgattgcggg agccgtactg ctccagccag ggcgagccgt cccggttctg
     3001 gataccgccg tttttccaca gggcttcccg gtcggggaag acggtgttgc acagccgggc
     3061 gttcacatag aaggccacgt ggccgcctgc ggcgatgacc gcgcgcacgc cgtcccgcag
     3121 ctcctcctcc gtgcccagat tggggttggg ccggtactgg gggaagccgt agtcgaagcc
     3181 gctctcgttc cagccgctga gcaggatatg gttcaggccg aacgccttgg cttcttcata
     3241 cagccgggga atatccttat aggtgtggac aacgccgccg gtctggtact ggaaatcgta
     3301 atgcgccatc aggccggggc tgcggtcgaa ccactccggg tgcagggggc cgtggccctc
     3361 cgcatccacg ggcacggcat agcgggtccc gtccggctcc gtcacggggg tggattcccg
     3421 ccacgtgcgg tagtcgtccg ccgcccagtg ccacgagccc tgatggggcg cgacgatgca
     3481 ttcctcgctg tgccacgtct gcccctccgt caggaacaga taatgcccca cggccacgtt
     3541 caggccgggg ttctccaggc cgaagctctc cgcccgcagg gtcttcatcc ggccggagac
     3601 gttgcggcag gtcaggtaca ggccgtcctg ctcgttttcg tcgaacacgt ccagccagag
     3661 catggtgcat tcgccggaat agggcagctc ccacacggac gcgccccggc tgtccttcac
     3721 gccgcagggg ccgttgaggg cggtggcgta gtcgtattcc tgccacttcc aatgggcgac
     3781 gcggggcggg gccgtcaggg tctccaccgg gttcatcacc ctctggcccg cgtggcgggg
     3841 catgtacagc gcgtcgtcct cccacgtttc tcccagccac aggccgttga cgcaggggaa
     3901 cagggcgcgc tccacctccg ggccgccgtg gttttccacc gtcagggaca gatggatccg
     3961 ctcgtcgccg ggcagaagcc gggcttccca gcggacggcc acgggcacg
//
LOCUS       DAWAEA010000096         3980 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3499_length_3980_cov_2.532484, whole genome shotgun sequence.
ACCESSION   DAWAEA010000096 DAWAEA010000000
VERSION     DAWAEA010000096.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3499_length_3980_cov_2.532484"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          690 a         1332 c         1144 g          814 t
ORIGIN      
        1 tgttgggctg caccacttgg cgcaggatgg gcgtggccac gttctgcagc aggtcgatgt
       61 cggtcttcag gccgatgatc tgctgcagat actggatgga ctcgttcacg ttggtctcgc
      121 ggacggtggt gttcatggtg ttttccaccg cggcagtcag gttggagcac atttccgtca
      181 tctgctcctc cgtggcccgg agaagcgtgt ccagctgata atccgccagc gtcagctggg
      241 tcagcaggga gcgggcgtat ttcatctccg cctcggtgaa ctgatagctc ttctgcttct
      301 gctgatcgtc aggggcaacg gtctgcagca cccgctggcc gtacttctgc acggctgtgg
      361 cctgtgtcag gacggactgc aggtcctgca tcacctcggc ggagataccc tccttgtaga
      421 cgtaggtagg ctccaccgag gcggcggcgg cttcctgctg ccgggtggtg gagatctcgt
      481 ccaccacgtc cttggacgcg gtgatggtag tgggggcgat gtcccccacc ttcaaattat
      541 agcgctgggg cgtgaccgcc agcatcagca gcaggcacaa aaccgctgtg cccgccagaa
      601 aaatatacac gcattgagcc gtggaggaat gcagggcgcg ggaaagatcc tcccagctcc
      661 attttttctt tttgggattg cgcttggcgc gaatcatgct ttagcacccc tgtttctctc
      721 ataggcctca taggccttca ccacggtctg caccagatcg tgccgcacca cgtcggactg
      781 ggaaagccgg acgatggcga tctccggaat ctctttgagc acctccaacg cctgcagaag
      841 cccgctctgc ttgccgtagg gcaggtcggt ctgggtcgcg tcgccggtga cgaccacccg
      901 actgcctgcg ccgaagcggg tcaggaacat tttcatctgt tcgggcgtgg tattctgggc
      961 ttcatccaga atgataaagc tgtcggacaa ggttcggccc cgcatatagg ccagcggcgc
     1021 tacttccagc gcgccccgct cctgaagccg ctggtagctt tccgcaccca tcatttcata
     1081 cagcgcgtcg aacaagggcc gcaggtaagg gtcgaccttc tggctgaggt cgccgggcag
     1141 gaagcccagc ttttcgcctg cttccacggc ggggcgggtg agcacgatgc gctccacctc
     1201 tttattgcgc agggcggcca cggccagcgc catggccaga taggtcttgc cggtgcccgc
     1261 cgggcctacg gcgaaggtca gctcgtgctc ccgaatggcc ttcacatagt cgtactggcc
     1321 cagcgttttg cagcgcaccg ggcggccccg gtgggtgatg gccaccacct gcgcggccag
     1381 ctcgtccagc cgatccagac cgtcctcctt caccagcgcg caggcatagc gcagggtgat
     1441 gcggtcgatg ggcgtatggc gttcttccag ctccatcagc ttttccagca cctgcagggc
     1501 ccagcccacg ttttccgcct cgccctcgat cttgatctcc gagccgcgca ggctgagctt
     1561 tacgcccaac tcgccctcca gaagcgacac attgcgctcg ttggcgccaa acagcgccag
     1621 ataagcgtcc attccattca gcgtcagata ttcctgaacc gtttccgtca aagatccagc
     1681 ctccgatcag gtggcgggct ttccaatttc cgtccgccat tcgccggtca tggacacggc
     1741 gaaaaattcc ccctctatca tacaataatc tacccatttg tcaatgatct catcgccccg
     1801 aagggcttct ttcaagcgtg tttccgccgc cgcgagggct tcccgtcggc actcctcgcc
     1861 gtccgcccgg acacgttcca gcgccgcctc ccggtaaagg agcattttgc gccagacggg
     1921 gaaaaaaacg cctgccaccg gctgcacgct ctcgtagagg ttcatggcga ggtactccgg
     1981 cttttccgtc tccgccggga actggaacca cggcgtacac agctgcgtca ccaccgtttc
     2041 ccgccccgtt tcccagcttt tcacctgatt cagcggcacc cgcacccgga cggtgcgcca
     2101 gcagcgggcc gtaacgctgc cccgggcctg caccgcgttg accgtctcgt cccggccccg
     2161 ctcctgccca cggatcagca cttgcccttt gcggacaacg gctcccggac ggacggcggc
     2221 agtcccggca aaaacctcga tcgtctccac cacgccgtcc cggctggcca ccagatcacc
     2281 gggctggccg gaatccagtt ccggcatggg atgaccctgc gtgcattcca cccgcagggt
     2341 catcccggcg gcatagacgt gaaaccacgc cgtttccggg taacgccagc tgagcttttc
     2401 ttcccacgca gccgcgtcaa gacccgcccg cagcattccg gggcgaatcc cttcctccgc
     2461 cagataggcg gcgatatcgg cctgataggc ccccgcgccg acgatttcca cccgccagat
     2521 acgctgactc ccgaggagca gcatcagcag cgccagcgcc ataccgaccc acagtccggg
     2581 ccgcgctttc cagctgctcc acactgccga aacccgctcg gggcgtactt cccggatgcg
     2641 ccagcccttg tccgcagcga tctgcgtcac tgcggcaaaa tccgccgtcc ggcactggca
     2701 gcgcagggtg cgccgtcctg tgcggcgcac atcctccaac gggatttctt cctgctgcat
     2761 cagcgccaga aaccgttcca gattcagacc gcacaccgta aaggagcatg tcagcagggg
     2821 agatgagagc tttttcattc cttcacccct ttggccggta ggagagcttc tcgatcttgc
     2881 ccagcaccac cagcccgtcc cgatcgtagc gggacagaga caggttttcc ccggtgatcg
     2941 acatcagccc gcactgggtg cgcaggacga tctcctcctg cgtacaggca tagatgccct
     3001 gatgctgctc cacaatgagc ttttcgtccc ccagcagtac ggagcggggc cagcccagca
     3061 ccagctcttt gggaatttcg ttccactttt ccagcatccc ccggcctcct gcccatggtt
     3121 ttgggcaagg atatgcgcga aaacaggcga acatacgcaa agacccggcc cgcctttttt
     3181 caggcagacc gggtgcattt tatcggctat tcctcgctca tgcggtagcc gatgcctact
     3241 tcagtaaaga tatatcgggg ttcgtccggg ttcggctcga tcttccggcg gatattggcc
     3301 atgtggactc ggagaatctt gttatcccga ctggcatagg ggccccacag ctccttgagc
     3361 atcgcccggt aggtgatgac ttggccggcg tgtttgccta gcagcgccac gatgcggaac
     3421 tcgttcgggg tcagccccac atccttcccg gccagataga cccggcattt gcggtaatcg
     3481 atcagcaagt cgcccacctc ataggagccc ttcagggcga tggaatcgtc ttccagcgcc
     3541 gtgcgggtgt ggcgcagggc ggtgcggatg cgagccagca gttcgatggt tccgaagggc
     3601 ttctgcagat aatcgtccgc gcccagatcc agcgcgtgtg ccttatcctc ctccatgctg
     3661 cgggcagaga tcacgatgat gggcgttttg gcccaggagc gtacccggcg gatgatctcc
     3721 gtgccgtcca gatccggcag gcccagatcc agcagaatgc agtccgggca gtgggaggag
     3781 atcatttcca gcgccttgac gcccgtgtcc gtccatagcg cctcataatg gttggtggcc
     3841 agcgtcgtgc ggagaaactt gctgatgccg gggtcgtcct ccacgatcag gactttgcag
     3901 ggcgcgctat tcgccatgtt cttcctcctc ttctctcggc agccagaacc gaatcaccgc
     3961 gccgcctccc tgccggtttt
//
LOCUS       DAWAEA010000097         3968 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3509_length_3968_cov_2.236903, whole genome shotgun sequence.
ACCESSION   DAWAEA010000097 DAWAEA010000000
VERSION     DAWAEA010000097.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3509_length_3968_cov_2.236903"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    334..346
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2816..2825
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3631..3640
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          838 a         1142 c         1245 g          710 t
ORIGIN      
        1 accttgtgat agtacagcgg ccgcagcagg gcgatgatga gctttacgcc aaagcggttc
       61 ttgtgacgct tcaccaccac gttttccagc tgctcccgca gggcggggtt gctgccgcct
      121 tcttccttca gggtcagcca gcgggccagt ttggcaaaat gccggttgtt catgggaaag
      181 cgcagcacgc atacgatggc catagccagc aaaagcagcg gcggcacgat catccagcgg
      241 tgcagcgcca tggaaagggt ctctagggtc agcgtttgcg gcaggaagcg gggcagacac
      301 agaaacgtca ccatccccaa tgcggccatc tgcnnnnnnn nnnnnnggtc atagccgtcc
      361 atccggcctt ccagcccgaa tcgggccacg tccgccatgc ggttttccag atgagtcagc
      421 gctgtcatgc tcatgcccag cccgccgccg cacaggcaca gcgccagata actcaacgtc
      481 aggctggtag agggcagctt cagctgccgg tcgaacagaa tcagcccata ggcccagaga
      541 aacagcccgg ccaatagaat ttgcaccgaa ccctcccgac gcagggcccg gagcaccgcc
      601 tccgccgccg cgtgggtgac cagcgtgcag agcgcagcca gaatcgtgca gagaaccagc
      661 tcgtccaccg tcaggccgat gaacaggtac gccagcacca acgtcagctg cagggccatc
      721 agaacgatga actgcagctt ccgataggcg ttgaaggcgt tcagctggtt cagtccgacg
      781 ttcagctcgt tcagcgtttc gggggagggc gtttcttcca gctcgtccaa ggcgatggtt
      841 ttccggctgc gccagtggct gaagccctcc agcgccgcgc ccagaacgta accgcataga
      901 aggagcaccg cttcattgac ggttaggcag cttagcagcg gccatgccgt tagccccagc
      961 gacagcaggt ggcacagaaa gcacagcccc cagaacgccc gcgggctgac tgtttcccgg
     1021 agccggcggc cccaaaggcg gctgcacaga ttttcccgca gggcggcagt ggcgacagcg
     1081 gcaaacagcg cccacagcgc cgccgaggac agcgccatgg ggtaaatggc gatcatcccc
     1141 agcgtgccca gcagcatcag cacggtcagc gcaaggcaga cccggcgcat ccggcgggtc
     1201 aggaacgccc gatcacggcc ccgcagaagc cgccacagca tcagcgagac ctgactgttc
     1261 agaaacagcg cgcagcccag caccggcagg gacgttaacg cgctggaaag aatggaagcg
     1321 tacaaaaatt gataaatcat ccgaagggac atcattgccg ccatgtccgg gcgggccgtg
     1381 cggataggag aacggctggc ttcagtcgag gcttgcaggg gggctcctcc tctcatcagg
     1441 aaacagatgg gaaataacag gaatgacagc gtctattgta acatagcctt ttccggaaag
     1501 gcaagaagtc cctgaacacg gccataggga aggcatattc gcccccttcg ccgcatacct
     1561 tgatacaaag ttttaggagg aatgcaggat ggagcggaaa caggacggcg gggtccagac
     1621 cccggaaatt cttagagaag aactgcccat gcagcgcttt gaggggcaaa agaccagtca
     1681 ggcggtggtg gagggagaag tggcgcttcc cggcggcctg cgggaggaga cccgcgtcct
     1741 gtgctccgaa gccatggcgg tgctggaaca gacggatgtt cagccggggc gggtggacgc
     1801 ggagggcaaa gtcaccttcc atgtgctcta tacccagggc gacccgacgc tggtcagcgc
     1861 gttggaggcc tcagcggagt ttacccacag cgtagatttt cccggcgcgg aggacggcat
     1921 ggccgccgtg ctcagcctga gcgtggagca cgtggaggcc agcgcccagg gcgggcggct
     1981 gcacctgatg gcgattttga aggcccagac ccggcttttc tcggatgagc ccctttccgt
     2041 ggtaacaggc gttcacgggg ccgaagggct gatgctgaaa acggagacgc tctcctgcct
     2101 gagcgcggcg gcttccggca cccgggaggt gctggtgcgg gaggaatgcg agctggccga
     2161 cgttctgcag atcgcggaca ccctgtacgc aacggccttc gccaccgtgc aggacgtgat
     2221 gggcggcgag gaaaaggcca cggtgtctgg caatatcctg ctggaagtgg ttcaccgcag
     2281 tcagatgccc tcccggcccg tggtcgtgac ccggcacaca ctgccctttg aggagacggt
     2341 gcctctgctg ggggaaaacg gcgatagcct ttgctgcgac gctgtggtca aggacgtggc
     2401 ggtgctgtcg caggagggga ctggcgaggg cgaacgcact ctgcgggcgg aggtgctgct
     2461 gggactgacc gtgcgagcca ccgccagccg ggacgtatgc ctgatgctgg acgcgtacac
     2521 cacacagggc gatctgctgg agcctgtgac ccagcctgtg cggcgcgcac tgaagcatca
     2581 tcagctgcat acggcggaaa gcggcaaaat gacgctgctt ctggatggtc agcccccggc
     2641 ccgcacgccg ctccgtgcca gcctgcggcc catcgtgacg gatgtgagcc gttccggcgg
     2701 caaactgaac gtggagggca tgatggaagt cacccttctg tacatgacgg acgacagcga
     2761 cgtgccccag acctatcaga cggaggaacc cttccgcatg agctacacct gcgagnnnnn
     2821 nnnnnttctc tggcaccggc agggataaaa aaacagcgcc atgcgggaaa tcgcatggcg
     2881 cttcttatat atgtatgttc ggaaaaatca ggcgtgcttg cgatggcgaa gcagcacgaa
     2941 acctgcgccc gccagaacca gcagtccggc ccatagcagc ggcgcggcat ggtcgcccgt
     3001 ctgcggaata gtttcgacta cggtaaaggt tctgttgtaa ttggaggaat caatatcgag
     3061 ttctctctcc gtaaaggttc ccgcttccgt ccgatacctt accgccgcgt tgtaggttcg
     3121 cacctgatag gtaccggccg gaagattgta agggatcagg acggagattt gacaggtaaa
     3181 ggaattttcg cccgtccacg taatgctcgt gtattggggg tctttttcaa tgtaggcgtt
     3241 ctttacggtg gaagctctct tgccggtcga gagatcgacc aatacaaatt cgctgtcgtc
     3301 ggtcagattc acttcctcca gcgtaagatt tttcgcgggg agagcgtcaa taggcatcgc
     3361 gctcggttgt tccagcagca gacgaagctt gccgctggac ggcgcggcgg taacttcgcc
     3421 gcccactacg tcgatattgg ctttgataca gcccgtcatc atcacggcgg agagaagcag
     3481 caaaaacaga agggaaacaa cgcgtttcag ggtgcttttc aaggggaaga ctcctttcct
     3541 aagcaaggga aaaatggaat atggctattt ttcagcatag cataaggaaa aacgattcgc
     3601 aagctcgttt tgcacttgct tttttacttt nnnnnnnnnn gcaggcgcgg cgttttctgc
     3661 tgctgcacca cgggctgatc ggcgcgtatc gtttccgggg aaagcagggc gcgtatgatt
     3721 ttgtgcgtca ggcgggctgc atccagttcg acccggtaga tgtgtgcggc aaaaacgccg
     3781 agctgacgct gcaatcccgg gtacagggct ttacgaagca gaccctctgg cagcttttgt
     3841 accgtgaccg gctgctggtg gattatcccg ataagaatct gtctatcctt ccaacagagg
     3901 actggcccta tttcgagcgc taccgccgca gcgcccggga gggcggccgg cagttcgagg
     3961 gtcttgcg
//
LOCUS       DAWAEA010000098         3961 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3520_length_3961_cov_2.060164, whole genome shotgun sequence.
ACCESSION   DAWAEA010000098 DAWAEA010000000
VERSION     DAWAEA010000098.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3520_length_3961_cov_2.060164"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2737..2746
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          786 a         1132 c         1233 g          800 t
ORIGIN      
        1 agttcacctt ctttctggct gtgcccgtca tgatcggcct gagctttatc aaaattctga
       61 aattcggctt tgccttcacc agcagtgaac tgatcgtact gctgctgggc tcggcggtgg
      121 cgttcgtggt ctccctgctg gtggtgcgct tcctgatgaa ctacatccgc aagcacgact
      181 tcacggcctt cggctggtat cggatcgcgc tgggcattgt ggtgattctt gcgctggtgg
      241 tgctgaaata aggacgctgg aatacgcccg ccttcctctt ggggaaggcg ggctttgtgc
      301 tgccctgctc gaaaccgctt tcattcgccg ggcttgacag ggtgaaggtt tctgagtaag
      361 ataattttca tcatacaaac gtttgcgcga agaggcgcag ccgagagacg gaaaggatgc
      421 ggtgcgcggt gaaatgtacc attaaagacg tagccaaggc tgcgggggtg tctccctcca
      481 cagtcagccg ggctttgcat aataatccgc gtatcagcga acaggcccgg gaacggatcc
      541 gcgcgctggc caaggagatg ggctttcatc ccaaccagat ggcccgcagt ctggtcaacc
      601 gcaggacgcg ggtgatcggg atcgttttcc cggccaatct ggccgaaagt ctggggcacc
      661 cgttttaccc gcaggtgctg caggggctgg gacagaccgc ggcggaacgc cgccactatg
      721 tgctgctggg cacggggcag ggtggcgaga ccacgccgga agccattcgt cagctggtgg
      781 acagcggcta tgtcagcgga ctggtgctgc tggctgccga ggaggggatt cagcccccgg
      841 aggacgtgcc cacggtggtc atcggccatc cctgcggcgg gggcctccat tacgtggatt
      901 ccaacaatct ccgggccggg cagaccgccg cagagtatct catccgccgg ggacaccggc
      961 gcattgccct gctgggctat gacaagcagt atatggtgac cacagaccgc cgggcggggt
     1021 acgagcaggc gctgcgcttc aatggtctgc ccctgcggga ggattatatc gtcccaagcc
     1081 gctacatgga taacgccacc gatcaggcgg cattgctgcg gctgttccgc cagcccgatc
     1141 atcccaccgc cgtggtctgc atggacgacg cgcaggccat cagcctgacc cacaccttaa
     1201 gcgccatcgg cctgaaggtg ccggaggacg tgagcctgat cagcttcaac aacaccgagg
     1261 ccgggcgctt ccatcatccg cccctgacct cctttgacgt gaacccctat cagctgggcg
     1321 tggaggccat gaacatgatc ctcgatctgg tgaaggaaaa gcccgtctcc gccagccggg
     1381 aagtgccctt taccctggtg gagcgggaaa gcgtccgagt gctgaaggac tgacaaaaag
     1441 caaacagaga ggaaaagatc ggaaatgcgc aacattcatt ttggctccgt ttatcatatg
     1501 ccgtggctgg aataccgcta cgccctgccg gatggcagcg tatgtctgcg gcttcagacc
     1561 ggccgggatg agtttaccaa ggtggaggct cgcgttacca gcaattacga catgcccgag
     1621 tttttcctca aggctcacgt gtacccgatg cagccggtgg cgcaggacga atggcacacg
     1681 tggtatcagg ttacgttcat gccccacgac cggcggctga aatacctttt tgtgctgcag
     1741 agcgacgagc aggtgttcaa gctggacgcc tccggcctac actgcggcga ggattatccc
     1801 gaggacgtga gcgaggcgtt cgcctttgct tacgcttatc ccaccgagcc tacgccccag
     1861 tgggcccgcg gctgcgtggg ctacgaaatt ttcccggacc gcttccgccg gggcgaaggc
     1921 tctcagacgg acgaaacgct ggagccgtgg accagcgacc gggtgcagaa cgaataccgc
     1981 ttcggcggca acctgcgggg cattatcgag gccgtgccct atctgcacga cctgggcgtg
     2041 gaaatggtct acacaacgcc cctgtttttg agcgacacct cccaccgcta caacaccttc
     2101 gactattatc aggtagaccc gctgctgggc acggaggaag acctgcgcga gctggccgac
     2161 gtgctgcaca tgaacggaat gcgcctgatg ctggacggcg tgttcaatca ctgcgggctg
     2221 cagttcgccc ccttccggga cgcggtggaa aacggcgaaa attcccgcta ccgggactgg
     2281 ttcttctttg acaataccga agaatgcggc tatcagacct tcggccactg gccctatatg
     2341 cccaagctga atctggccaa cccggactgc gccgcctatt ttctggacgt gggccgctac
     2401 tggctgcgct cctgccacat cgacggctgg cggatggatg ttagtcccga gatctggccg
     2461 gacttctggc gcaagttccg caacatgctc cgggaggaaa atcccgacgg gttgatggtg
     2521 gcagaatgct gggacgacag ccgccagtgg ctctccaccg gcgacatgtt ccattccacc
     2581 atgagctacg tctggagccg caatgtgtgg aaccgcttct gctaccgcaa gatcagcctg
     2641 cgggagttcg acgcggtgaa caaccgctct ctgatgctct atcccgaacc cgtgacgcag
     2701 gttttgtgga acttcctgga cacgcacgat acggccnnnn nnnnnnttcc ccggcagtcc
     2761 cattctttac tacggcgacg aactggccat ggagggcgcg gacgatccct tttgccgttt
     2821 tcccatgccg tgggaacggg tgaaatatag cgatatgcac cgctattacc ggtgccttgc
     2881 ggaaattcgc cgggagcacc ccgtgctgcg ctacggctcc ttccgcacgt ggcaggtgca
     2941 ggaggatggg ctgtacgcct atctgcgcgt ggcgggcggg gaaaccgtcc tttgcgtgct
     3001 gaacacatcc gataagccgg tacacgggct gctgcccctg ccggagagca tggcggagca
     3061 gagtgctctg aacgaattga tgcattcttc atccgtcgcc gtgcgcgccg gatgcctgca
     3121 ggttgccctg cgtccgggcg agggcttgat tttctgctga atccaaagta caatacctct
     3181 atcagaccgc aaggaggatt ttttccatga aagagctggt agaggccatt cgcacttatg
     3241 gcaagggcat tggaacggat attgtcaagg tggatatgtt tctcaaccac cggctggata
     3301 caaatttact ggtcaaaatg ggtcaggctt tcgccgatgt ttttgcgtgc gaaaagcctg
     3361 accttgtttt gaccatcgag gccagcggca tcgccgcggc gctgactacg gccatggcct
     3421 gcgggaacat tcccgtggtc ttcgccaaaa agagcagcac ccgcaatgtg accggcgacg
     3481 tatatgaatc gacggtcttc agctttaccc acgggttgga aaaccacgtc cgcgtggcga
     3541 aggaatatct acccgccggg gcgcgggtct tgatcatcga cgattttctg gccaacggcg
     3601 aagccgccga ggggctgtgc gatctctgcc gtcaggcagg tgcgaccgtc gtcggcatcg
     3661 gcatttgtgt ggaaaaggcg tttcagcccg gccatcagaa gctggagaag gacgggtaca
     3721 aggtacacgc gctggcgaag gttttgggca ttgaggacgg aaaattgctg gtagaggact
     3781 gaaataagcg cagcggcggt gagtggaaac gctgctaagg ggaaatgccc cgttggagaa
     3841 ggaagcgcag cttcggcgca ggagtgggac ttgccggttt atgggtttct cgccaacttt
     3901 ggtgctcgaa ggtttccaaa ggggaacgaa gggaagacgc aaggcgggtt gcccttgggc
     3961 a
//
LOCUS       DAWAEA010000099         3941 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3551_length_3941_cov_1.641019, whole genome shotgun sequence.
ACCESSION   DAWAEA010000099 DAWAEA010000000
VERSION     DAWAEA010000099.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3551_length_3941_cov_1.641019"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    379..403
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    845..854
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3279..3396
                     /estimated_length=118
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          755 a         1077 c         1193 g          763 t
ORIGIN      
        1 ccttctggca gaagccccgc gtggtcatgc tggcgctgac cggcagcttc gcgctgctgg
       61 tgctggtgct gatccccggg atcggcgtgt atgtgaacgg ttcccggcgc tggctgaaca
      121 ttgcgggcct gagctttcag ccgtcggaac tggcaaaaat cgcctcggtg atgtatctgg
      181 cctccacgct cagctttcgg ctcaaacagg tggagcggct gtggacgggc attcttcccc
      241 tgctggtgat cccggcggcc atgtttctgc tgattttgca gcagcccaac ctgtccactg
      301 ccgggagcat catgatcgta gccatattga tgatgctgct ggccggggcc aaatggaaac
      361 atttgagcct gatgggcgnn nnnnnnnnnn nnnnnnnnnn nnnccgggga ctggggcagg
      421 gacggcagaa gttttcctac ctgccgtacc cggaaagcga cttcatcttt gcgattatcg
      481 gagaggattt cggcctgttc ggctgctgcg tggtcattct gcttttcgtg gcctttgccg
      541 tggcgggaat gcgtattgcc ctgaactgtc aggatcgcta cggttgtctg ctggcggcgg
      601 gcattaccgc catgatctct gtacaggcct ttatcaacat cggcgtggtg gtgggcattc
      661 tcccaacgac cggtcttcct ttgccttttt tcagcgcagg cggcacctcc atttcccttc
      721 tgatggccgc cgtcggcatc ctcttcaata tttcccgtca tgcatgaaga cgttggggcc
      781 tttgcggccc caaaccctgc agtaagggcc ttgcccttaa caagctcatg atcgaccgca
      841 cattnnnnnn nnnncggcgc tctcacacat ctcccggcag cgcataggat gggtcatgcg
      901 atgcttgacg gaggtggaaa tatggacgcc ttacggattc ggggcggaaa acgcctgagg
      961 ggcgaatgga cggtgcagaa cgcgaaaaac gcggcgctgc ccatcatggc cggagcgatt
     1021 ctgacggagg ggaagaccgt tctgcgggac tgcccgcggc tcagcgacat cagcgatatg
     1081 ggggaaatcc tccgaacgct gggctgcggc gtacataggg acggcggaac gctggagatc
     1141 gacccgggcg ggctgtgcgg ctacgaaatg cccgaagaac tctccaaacg catccgttct
     1201 tccatttttc tgctgggccc cattctggcc cggttccgca gggccaacgt gtccctgccc
     1261 ggcggctgcg agatcgggct gcgtcccatc gacctacacc tgagcggcct tcggcagttg
     1321 ggggtgcaga tggaggaaga aggcggcgtt atccgctgcg acgggcgcaa catgcacccg
     1381 gctaccgtcc atttcgacta ccccagcgtg ggcgcaaccg agaacgtgat gatggcggct
     1441 gcattgcttc ggggcgagac cgtgctgaat aatgtggctc gggaaccgga aattctggac
     1501 ttgcagcgct tcctgaacgc catgggggcc aagatcaccg gggcgggcac caattccatc
     1561 cgggtgcagg gcgtgtcccg attgacctgc gtggaatacc gacccatgcc cgaccgtatc
     1621 gccgcaggaa cgctgctggc cgcggctgcc gtcaccggcg gaagcgtccg actgtggaat
     1681 gtgccggtgg aggaaatgct ggcggtgatc gccaagctgg aagaaatggg ctgcgtgatc
     1741 tcccggcagg agaacgccct gacccttcac ggccctgagc ggctttccgc cttcgctcag
     1801 ctgcagaccc agccccaccc gggtttcccc acggatatgc aggcccagat gatggcgatt
     1861 gcttcgctgg cgaagggaac ctccgtggtg gtggagaatg tatttgagaa tcgttttgct
     1921 catgcgggcg acctgaaccg catgggggcg aatattctga tcaacggccg cacggcggtc
     1981 attcgcggcg tggagcgcct gagcggcgca cgggtcacgg cacgcgacct ccgcggcggc
     2041 gcggcgctgg ccatcgcggg ccttgcggca cagggggaga ccatcgtgga acgggcccgg
     2101 ctgatcgacc gcggttacga gagcttggaa acggccctga cccatctggg ggccgatgtt
     2161 cagcggattt cagtggaaag aggagaggaa cacccatgaa acacaaacgg aacgcccctt
     2221 ccagacagga gcgggaagaa tttgaagcct atggcgagta ttcctatgcc tatctgcacg
     2281 accggcagac aaaggagggg cagcccgacg aacccgtcag cccgccgcct gcctcctccg
     2341 ctgccgccag tgaaaagcgc cgccagaaac ggcgcagggg cgtattgatc aaaatggtgg
     2401 tgctgctggc ggcgctgtgc atcctgatca tcgtgctgca ggagacggtt tttcggctgg
     2461 acgcggtgta cgtcatcggc ctgaaggagc attctcccca gcaggtggtg tcggcttccg
     2521 gcctgtacaa gggccggaat atcttcaccg tcagtgagga cgaaatcgcc aaaaatctgg
     2581 aaaaggatta ttccatcatc tatcagggaa tgcagatcga ataccccagc accatttacc
     2641 tttatgtgga ggaacgcaag cccgtggccg tcatgcgctg gctgggcagc cagtacgagc
     2701 tggatgcgca cggcatggtc atgagcgaaa gcgacagcat gacgctgccg gaggggatgc
     2761 cgctggtcac cggcttccgc gtcacggcgg tgcacgtggg gcagaccctt tccctgcgca
     2821 gccagaagca gatggacatt tactgccagc tgctcagcga actctcttta caggaatatg
     2881 cggatgaggt cacggaaatc aatctttccg atacggaaaa tctttatttg atcacgcagg
     2941 agggcatttc cgttcgcatg ggcgacggag aatacctgcg cgccaaggtg ggggccatcc
     3001 gcacctatat cgcctacttc cggcagctgg gcaccgattc cggcgttctg gacgtttcca
     3061 tccctgaaga cggaaaattc atgcctgaac ggtaacattt tttctcatca aagacggaat
     3121 ttacaaaaaa gtgaaaaaaa agtgggattt ggcttgcgat ttcctgttaa aacgggtaca
     3181 ataaatgctg gtacattggg tgccctgttt gcgtaacctt ttgtttgttt caaagcgccc
     3241 aacgcctgcc gtcaggggtg cctgcggctg attccagann nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntggc acgtcgaaga tcgtgacgct
     3421 ggtggccgaa acaagcggct ctcagcggtg cgatattgtc ggcgcgggga tttccatgta
     3481 cgacggcttt ttggacggcg agtggaacaa taccatggcc ctgaacgagg ccattcaaaa
     3541 atccatcagc gaggcggaaa accagtccaa acgcaagatc cgcgagatca acgtgggtgt
     3601 gccaggcgag tttacccgcg tttacgcggt ggagaccagc gtgaccattc agggggccga
     3661 cccccgggta acgcctcgcc atgtggaaaa gatcttcgcc aacgcggaca agcgtctttc
     3721 tcctccaccg gcgaggccat gctgtatctg ctggaagaag accgcgaccg cacggcgctg
     3781 ctgatcgacg ttgggtatct gtgcaccgat gtgatggccg tggagggcga cgcggtgatc
     3841 ttccagaaga cgcttcccgt gggcggcgga catattgctg ccgacgtggc cgagggcctg
     3901 aatatttcgc tggaatccgc cgaacagatc aagcgcgaat a
//
LOCUS       DAWAEA010000100         3917 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3575_length_3917_cov_2.051269, whole genome shotgun sequence.
ACCESSION   DAWAEA010000100 DAWAEA010000000
VERSION     DAWAEA010000100.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3575_length_3917_cov_2.051269"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2031..2040
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          795 a         1134 c         1253 g          725 t
ORIGIN      
        1 agacggtgct gtctaccctg acggcggaac gcaccctttc cgggcagggc acgaaggaaa
       61 aggcccgggt agtgatcgag gaacccagca tcgccgggct ggaacggctt ggctcattcg
      121 aacggttgat gatctggatc gtggtgctgg cggtgctgct gggcacggcc atcgccgtgt
      181 acgcggcgtg gaggagctat cagcctatcc gcaagctgta cgccaagtac ggcggcagcc
      241 agaagcccag caacgaactg cagacgctgg aagacttgct gagtaactcc ctgaaacgaa
      301 acagcttttc ccaaaagcag atcgaggaac agatggaaca gctggacttg cagcaatcgt
      361 ggctgaagca gcagctggtg atgatgctca tcaccggcaa cgacagcccg gtggtcaagg
      421 aattgatcca gaaaatgggc ttcgaaatga gtcacgccca ttttgccctg tgcttcctgt
      481 atctgcaggg cgacgagggc gaccgaacgg gactggtgcg cagcattgag gatttctccg
      541 acgaggaatg caccctgtat gcggcggaat tgcaggaaaa ccgcgagtat atcgtgctga
      601 tgaactttca ggaggaagaa cagtgccggg agctgctgga acttttgtcc gacagtctgg
      661 aaagccggaa tttgtcggtg cgggtgcagt ggagccgcac ctgcagccag ctgaacgaga
      721 tcgcctccgc agccatcgaa acgctgaaca cgccgccggt ggcgctggcg gtggacaacg
      781 ccgaagccga ggaagaagac gcgctggagc agatggcggc gctgacggaa agcggaagca
      841 catcgcaggc gctggccctg ctggagacca tgatcgccca gacggaaaac cgttatccgt
      901 cctatctgat gcgcatttat atgctcaacc gtctggtgca gcagatgatg acgctggcct
      961 cccgggaagg cgtgaccctt ccgcccaagt ctccagggca ggacccggac agcgtacggg
     1021 aaacgctgga acagctggtg cgcgccctgt gccgcaaggg ggcgcagcgt tcgtccaccg
     1081 aaaagcccga gggcggcaag accgccgctt atgtgcggga gcattgtctg gacggggaca
     1141 tcagcctgtc ctccacggcg gaggcgctgg gcatttccac caagcaggtg tcccggctgc
     1201 tgcgcatgga ggtggacatg actttcaagg aatatctgct gcatctgcgg atgtccgccg
     1261 cgcaggattt ccttcgggag gaaggccttt ccattgcgga aactgcgggc cgggtaggat
     1321 atttcaacat ttcccacttc atcaagtgct tcaaggccta taccggcatg acccccggcg
     1381 aatggaaaaa gctttcgtcc gggcggacgg cagaataaga aacgcccttt cttcggaaac
     1441 ggtttgctcc gaagaaaggg cattttcgcg tggtcattcg atctcgttta ctcctgctgc
     1501 agcatccatt cgataaaaag tccgtagccc atggtagggt cattgacgaa tacatgcgtc
     1561 acagccccta tgagccgacc gtcctgcaga atgggactgc cggacatgcc ctgcacgatg
     1621 ccgccggtct tttgcagcag acgctcgtcc gtgacccgca gcaccatact gcgctgggcg
     1681 ggggaggact ggcgggccac ttcgacgatc tcaatggcgt attcctgcat ggaggcgccg
     1741 tctaccgtgc agagaatgga ggcggggccg gtgtggacgg cgtccttgcg gccaatgggg
     1801 agcccatccg ggtagagagg atgggcgggc gcactgtcca gccggccgta aatgccgaac
     1861 tgattgttca ggcggatatt gcccagcacc cggtgctctc gaagaaagct gcccttcaat
     1921 tcgcccggaa cgccggcctg ccccttgcgc acgtccacca catccgcttc catcaattcg
     1981 ccccgggaaa gggtcaaaat ctgctgagta tccgcgtcgg tgatggcgtg nnnnnnnnnn
     2041 cgtgccccaa cgcgccgtag cgcagcgggc cgttttcctc gacctgtccg taaaaggaaa
     2101 gcgtgccgac cccggcggta gaatcccgca cccatgcgcc gatgcggtag ctgccggtgg
     2161 ctccgtcctg ccggggttcc agcgtcagac gaagctcgcc gctgccccgg cggatggtga
     2221 ggggcatggc ccggctgccg ccctcgttga ccagcgccgt cagttcctcg atgctgtgca
     2281 gaggcttgcc gttgacctgg gtgatcacgt ccccggcttt cagccctgcc agccgcgccg
     2341 ggctgaaagc gccggtcagg tcgctggtgc ccaccaccag tacaccctcc gtctgcaagg
     2401 ccacgcccac ggcctgcccg cccgggtaca ggcgcagatc gtcccgaatc tccaccgtga
     2461 cctgacgcag gggcagaagc cccagcaaac tgacggtggc cctggtctcc gagccaccgt
     2521 ccagcgtgtc gccggtggcg gaaagggcct gcacgtccgg ctcgtccgcc gtcagccgca
     2581 ggggaaaggg aagctccgcc gccgtttcct gcccggaagc gatgaccaaa cgctccggca
     2641 gatgccgcag cgcttccgcc tggggagaat aaaagcccag cagccaaaag gcgcatagca
     2701 gccaccccgt cagccgtttt tttcgtcctg ctcttttggg tcctctcatg gttcaaacgc
     2761 ctctttccgc tttgcattgc acgaaagcat tgtttgcaga aagaggcgtt ttgaatgatg
     2821 ggaatggctg tcagttcgct tgaaaaatta taaatgcaga accagcgctt ttcgggattg
     2881 agaagggcct gatcccgttt ttggacgaaa gcggcggctg aagtacagcg cgtcccgaag
     2941 cccagccgag cggcttcggg gacgcttgtt tgcgaatagg gagtgttttc gaaaacgtcc
     3001 gtctcacgat tcctcgccgg aggcgtgcat ggaacggtat tcgctgggtg tgcagcccat
     3061 ttcttcctta aagacccggg caaagctgac cgcgtccggg tagcccacct tgctgccgat
     3121 gagatagaac ttgtcgttgg tctcccggat caggcggcag gcttcctcca tgcgcggatg
     3181 cttgcggtaa cggctgaagg acgtgccgca gaccttgcgg aaaatggtgc tgacgtagtt
     3241 gggatgaagc tccagatgct cggccagatc ggtcagggtg aaatcctcgg caaaatgggc
     3301 ctgaatgtat tcgcaggcgc gcttgacgtg ggtctcgtcg ctggtggtga tctgtccctg
     3361 tatgcggctg atctgcaggt cgatgatgcg ggtcatgctg cggtagagcg tttcggcgga
     3421 atgggtctgc tgaatggcac agatgatgtc gtccgccgtc agctccatct ggctgaacac
     3481 cgccacgaag gaagagtttc cctgcagggt ggcgtagatc ataccgcaga gaagctgacg
     3541 aagggaatcc accgggggat actgggcgca ggcccggcgc agcagaagca gcagcgtctg
     3601 atggtagctt tcgacctgca ggcggcagat cgcgtccgtc agatcggaga tcagcgtgga
     3661 atagggatag gtctccgggc cgtcctcacc gggcaggtcg aagaagcggc gttccagcag
     3721 cgtttggcat acagaaagct gctccggcgc gtggggcaga agaaacatgg gggaaagaac
     3781 ctgcgcggag ttccccggca gcagaccgcc catggcagta tactggggct gatcgggaaa
     3841 gatacacagg ctcaggcaca tctgccgctt ccggccctga aacagggcgc agcccgtctg
     3901 caaaagcggc tgaatgg
//
LOCUS       DAWAEA010000101         3917 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3576_length_3917_cov_2.030813, whole genome shotgun sequence.
ACCESSION   DAWAEA010000101 DAWAEA010000000
VERSION     DAWAEA010000101.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3576_length_3917_cov_2.030813"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2206..2215
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          702 a         1273 c         1110 g          822 t
ORIGIN      
        1 cgcgacgatt gagtggcagc ggcgcctcct gcacctccac ttttttgaca agctgaaacg
       61 tccccgggaa gaatcatctt cccgggggcg ttttttggat tcgtctttac aaaatcagca
      121 tcgcatcccc gaaggagaag aagcggtact tttccgctac ggcgatattg tacacccgca
      181 gggcttcttc ccggcccatc agggcggaga tcagcatcag cagggtgctc tggggcaaat
      241 ggaaattggt caccagcgcg tcggtggcct tgaaatggta gccgggcatg atgaaaatac
      301 ccgtgtcgcc gttttgggcg tggacgagac catcctcggt gctggcggtc tccagcgtgc
      361 ggacgctggt ggtgccgatg cagacgcagc ggtgtccctc agcccgggcg cggttgattc
      421 tctcagcggc ctccggcgtg acctcgtaat gctccacgtg catctggtgc tggctcacgt
      481 cttcctcctt caccgggcgg aaggtgccca agcccacgtg caacagcacc ggcacgatgt
      541 cgactccctt ctgacggatc gtttccagca gttccggcgt aaaatgcagt ccggcggtgg
      601 gggcggcggc gctgccctct tcccgggcat agaccgtctg ataccgttcc cgatcctgca
      661 aacgctcgtg aatgtagggc ggcaaaggca tctgtcccag ccggtccaga atttcctcaa
      721 aaacaccctg ataatgaaaa cgcaccagcc gcgcaccctc cggggcccga tccacgatct
      781 ccgcccgaag aaggccgtcg ccgaagctgc accatgttcc cggagccaac ctgcggcccg
      841 gacgcaccag cgcttcccac aggtcttttt ccagcctctt cagcagcagg atttccaccg
      901 gcacatgggt ttcttccttt tcccccagca gccgtgcggg aatgacgcgc gtgtcattga
      961 tgaccatcac atcgcccgcg ttcagaaagc ggggcaggtc gtaaaaatgc aaatgctgaa
     1021 tggagtgatc cgcccggtca tacaccagca agcgactgtg atcccggggc tccacagggg
     1081 tttgggcgat cagttcttcc ggaagattgt agtcaaaatc cgaggtcttc atattctcat
     1141 ctgctcctgt ccatcatccg caggctgctg gggcttgcgg cccatatgct cgtaggcggc
     1201 cggggtgacc acccgtcccc ggggggtgcg catcataaag cccagctgca tcagataggg
     1261 ttcatacacg tcctcaatgg tggtggcatc ctcgccggtc atggcgctga gggtgtccag
     1321 ccccaccggg ccgccgccga acttgtccat ggcggtcagc agcatggtgc ggtccagctt
     1381 gtccagcccc agctcgtcca cgccctgcat attcagcgct tctctggcca catccagcgt
     1441 gatcacgccg cccgcccggg tctgggcata gtcccgcacc cggcgcagca tccgattggc
     1501 gattcggggc gtgccccggc tgcggcgggc gatctccaga atgccctccg gctcggcgtc
     1561 cacgttcaag atgcccgcgc tgcggctgac gatggcggaa agctcctccg gagtatacat
     1621 ttccagccgg aacagaatgc cgaaccgatc ccgcaggggt gcgctcaaaa ggcccgcccg
     1681 ggtggtggct cccaccagcg taaagtgcgg cagatccagc cggatgctcc gggccgtggg
     1741 gcctttgccg atcatgatgt ccagcgcata atcctccatg gccgggtaga gcacttcctc
     1801 cacggaacgg ctcaggcggt ggatctcgtc aatgaacagc acgtcgccgt ccgccagatt
     1861 ggtcagaatg ctggccaggt cgccgggccg ttcgatggcc gggccgctgg tgatgcggat
     1921 gttctgcccc atttcagcgg cgataatgcc cgccagcgta gtcttgccca gtccgggcgg
     1981 gccgtaaagg aggatatgat ccagcgattc ccggcgtttc agcgccgcct cgatggaaat
     2041 atgcaggttt tgcttcacac tttcctgccc gatatactcc cgcagggtct ttggccgcag
     2101 gctctgttcc tgctcatcat ccccctgacg gaagcctgaa gatacgatcc ggtcgtccat
     2161 gccgttcctc ctgtcggttt atccctgctg tgccatgtgg cgcagnnnnn nnnnncttca
     2221 aggcagacgc ggcctcctgc ggcgaatacc ccagcgcctt gagggcctgc atggcctcgc
     2281 tgagcgcgtc gccggcgggc gcgtcgctgt ccgttacggc ggcgatctcc ttcagctcat
     2341 ccgcgccgct gagggcctcc ttggccagct tgtccttcaa ttccagactg atgcgctggg
     2401 cggtcttttt gccgatgccc ggagcccggg acagcgccgc cacatccccc gtcaggatcg
     2461 ccagccgcag gttggacagg ggcatactgc ccagaatttg cagcgcgctg cgggccccga
     2521 tcccggaaat agcgatcagt cgccggaaca tctcccgttc ctcggtctgc agaaaaccgt
     2581 acagctccat cgcgtcctcc cggacgttca ggtgggtgta caggcggcat ttgtccccgg
     2641 cggcgggcag ggaggacaag gtattcatgc tgcacatcag ccggtagcca acgcccccgg
     2701 cagtcatcac gatggcctcg ccctcgcttt tttccactac cgttccttca attaaagcga
     2761 tcatccgtat cgttcctcat ttcatgcgga attggccgcg ggccatcccg gcggcggcgt
     2821 gggtcagggc aatggcgatc gcgtccgccg cgtcgtccgg cttggcgatc gtctccatat
     2881 gaagcagcat cttcaccatc tgctgaatct gcttcttctc ggccccgccc cagcccacaa
     2941 tggcctgttt gacctgcatg ggcgtatatt cgtaaagctg atccgtatac tcggcgcagg
     3001 cggcgatgct cacgccccgg gccgcgccca cgttcagcgc ggtggtcacg tttttggcga
     3061 aaaacagctc ctcaaaggcg atctcctccg gctgaaagcg ctggagcagc gcccgcatgt
     3121 cctcgccgat ctgcttcagc cgctccggga agcgctgatc gggcgtagtc agaatacaac
     3181 cgtactcgat caggcgctgc cgtccgggct gcgcgtcgat cacgccccag cccagcgtgg
     3241 ccaaaccggg gtcgatgcct aatattacca tatttttccg tccttcccaa taaaaaaacc
     3301 aaaattctcc tgaaaaaagc cttgcatttc ttttcagggt ttgttataat ctgttttgtt
     3361 gcgtggggca ttagcacagt tggtagcgcg cgacattcgc attgtcgagg tcaggggttc
     3421 gactccccta tgctccacca gtaggaaccg ttcaaacggt tcttttttgt tgccagaaaa
     3481 agagtataac ggtatttttt cgggttgtca atcaaaaaaa gcctgtcccc acagcctttt
     3541 caaagcggag acaggctttt tccgttactt tccaacccgg aaatcttcga atatcgcttc
     3601 cccgaccccg gacaacgcca gcccgtacat accatgggtg tatggcactt ccgcttcggc
     3661 gattttctgt ccgtcgatga acaattccgt ctttgcgccc cgagcaacag ctttcagctg
     3721 atacggctga ccggctttcc agtcgaagtc tgcctctgcc agcggccgat agccctccgg
     3781 gctgcccagc cagattcctg cttttctcgg tgcggtcaac cccgccagta cgtgccgcag
     3841 ggtgcccatt ccccgcacca gcagacaggc tccgcccgac agcggcgttg accggcaggg
     3901 aagagtcaca tccccgg
//
LOCUS       DAWAEA010000102         3915 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3580_length_3915_cov_1.294301, whole genome shotgun sequence.
ACCESSION   DAWAEA010000102 DAWAEA010000000
VERSION     DAWAEA010000102.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3580_length_3915_cov_1.294301"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2515..2756
                     /estimated_length=242
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          729 a         1092 c         1133 g          719 t
ORIGIN      
        1 gcccctttac caagcgctgc aatcagcagc tggaggaaat gaccggcgcc tgcaaggcgc
       61 tgctgaccac ctccggcacc tcggccatgg agctggcggc gctgctgctg gacgtgaagc
      121 ccggcgacga ggtcattgtg ccgtcgttca ccttcgtcag cacggccaac gcctttgtgc
      181 tgcggggcgc gaagatcgtc tttgtggacg tggatcctgc caccatgaac atggatcccg
      241 cctgtgtgga ggctgccgtt acctcgaaaa ccaaagtcat cgccccggtg cattacgcgg
      301 gcgtctgctg cgatatggac gcgatcaacg ccattgcgga ggcgcatcac ctgaaggtga
      361 tggaggatgc ggcgcaggcc gtgagctcca cctacaaggg acgggcggcc ggcagcctga
      421 gcgacgtggg ctgctacagc tttcatgaga ccaagaattt cagcatgggc gagggcggcg
      481 ctaccgtcct gaaccacccc gagctggtgg aacgggccga gatcatccgg gaaaagggta
      541 ctgaccgcag ccgtttctac cggggtcagg tggacaagta cacgtgggtg gacatcggtt
      601 cttccttcct gcccagcgaa ctgaacgcag cctatctgct ggcccagctg gagcagcggg
      661 aaaagatcac cgcgcgccgc atggcccgct ggacggaata cgatcagggc ttgcgggact
      721 tggaggaaca gggccggctg gagcggatga aggtgcccgc cgagtgcggc cacaacgcgc
      781 atatgtacta catcaagctg cgggatcagg aacagcgggc cgcactgatt tccttcctga
      841 aggaacggga gatcagcgcc gtgttccact acatccccct gcacagcgcc aaggcgggcc
      901 gggagtttgg cgtgttcgcc ggggaggacc gctacaccac ccgggacagt gaacggcttt
      961 tgcggctgcc gctgtactat gaaatgaccg gcgaggaaag cgccaaagtc attgaatcgg
     1021 ttcatgattt cttccgtcag gagaaataaa ggaggaacga ttttatgttg aaaaggaatt
     1081 ggagcctgac cggggtcagc cttgcggcga tcatcttcta cgcggtctgc ggcctgctgc
     1141 tgcttctgct gccgtcggaa gcgctggcca tcgccaacta cgccattgcg gcgctgctgc
     1201 tggtgatggg cgtggtgtgc attatcggtt acttccgggg cagcgtcatc atcggccagt
     1261 tcggtttcct gctggcggtg ggaatggtgc tggtgacgct ggccgtggtg atgttctgct
     1321 atcccacgct gctggcgagc ctgctgcctg tgatctgggg cgtgtccctg ctgatcggcg
     1381 gcttcggcaa aattcagttt gccgccgacc tgcgccgctt cggcgaccgc cgctggtgga
     1441 cgctgctgct gggcgcgctg ctgtccttcg cgttgggcat tctctgcctg gccaacccca
     1501 tttacattgc ccagaccatt ttcgttttca tcggcatctg cctgctggcc gaagccgtgc
     1561 tggatctggt gacgctcctg atggttcgcc gtcatctgcg ccggtttgag aaggccatgg
     1621 aggtctgaaa atactcgttt tatccgccgt ccccattccc ggacgttcat gggaatgggg
     1681 acggtggttt tcgtaagtct ggaacgaacg gacgccacga caccctcgca aacgatggac
     1741 gctaatggaa aaccgccctc ttccccggca cttggggaaa gagggcggtt cgtttgtatg
     1801 ccggtcagcc cttgacgatc tccccggcct tggtcagggc gatcttggtg atgaccgggc
     1861 cggtcagctc gtaaatgagc gtgcccgcca gaatgatggc ctgaatctga cggccgtact
     1921 gaggcagctt ggtcagggtc atggtggcca gaccgatggc cacgccagcc tgcgggatca
     1981 gggtaaagcc cagatatttc tgcacgttgg ggtgagcgtg taccatccgg gaaccaagca
     2041 tcgcgcccag ccatttgccc accacgcgga acagcacgta gcaaacgccc acaaggccta
     2101 ccgtgggcag cacgcccagg ttcagctgtg cgccggagat gacgaagaac agcaggaaca
     2161 gcggcggcgt gaagcggtcg gtctgctcga tgaccacatt ggcggtcttg cacacgttgg
     2221 tcatcaccgc gccgatcatc atgatgcaca gcaggttgga caggccccac agtcagcgcc
     2281 agcttgttgc cccgggaatg gaagaagctc atgcacaggc tcagcacaaa gccgatgacc
     2341 gtgccgatga ccagcgagcc cacgatctcg cccagcggga tgagtgccat gttgactacg
     2401 gtcatgggcg cgccgctgac catggcctgc gccacgctga tggagatggc ataggccatc
     2461 aggcccagcg catcgtccat ggccaccacg ggcagcagca tttcgcacac cgggnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgcg
     2761 gcgggggccg tggcggaggc gatcgcgccc agcgtgatgc acatgggcag gtcaaagccg
     2821 ggtacgccgc acagggacag gatgatcagc acgatgtcca cgatcagcac cgcgcccagc
     2881 ccctcgcaga tggtgatgac cacgggcttt gtgccgatgc gctttaagta gctcaatttg
     2941 aactccgcgc cgatggagta ggcgatgaag cccagcgcgg cctcggtgat gatgctcagg
     3001 ctgtccgtca ttttctgagg aagaatgccc agtacggagg ggccgatgag cagaccgccg
     3061 atcagaaagg ccgtcacgtt gggcatatga accagcttaa tgaaacggct caccagcaga
     3121 ccggccataa ttgcgatgga aagcgccaat agggtcgtca ttgctgattg gcccccaatc
     3181 cttccacaaa tagggcgggg accgcaaaca taatgccggt gtccgggtga tccagaccgc
     3241 ccaccacctg acgcaccacc agcttggctt tgtggacttc atcctcggcg agcaccatca
     3301 tcagagtctt gctgctttcc cgggccgggt caaaaagctg acgcagcgcc ccgaacatgg
     3361 gcaggtcgtc cccgcttttg tcaatgacgc gcatcatgcc catactgtcc agcacggtgc
     3421 agccgtgaat gccgctttcc agcagccgtt ccaacagcgt ttccaggtat tcggttcgat
     3481 tgagcaccag aaatagtacc tgcataaatt tctctccctt gccaaaatcg gacggactgc
     3541 acgggcttcc ggcaaccgcc gcaaccattt attatagatt gtttttggca taatgcaagg
     3601 actttcggca ctttttagcg ctttattttt gcgggtttcg ggcataaacg gagggggaga
     3661 cgcccacgat gcttttgaag acccggttca tacccgcaaa cttcccgaac aggcggcgga
     3721 agtagcactc actcatgccg cacagagccg ccagttccga tacgggcgtg ttctcgttcc
     3781 agtggagctt cagatattcc acccctgccc ggatgggata acgcttttcc gattcgccca
     3841 cacagaagga cagcacgtgc cggagcagcc gatacgcctc ggctttgacg cacaccatgg
     3901 agcgtttcgg cgcat
//
LOCUS       DAWAEA010000103         3895 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3596_length_3895_cov_2.150260, whole genome shotgun sequence.
ACCESSION   DAWAEA010000103 DAWAEA010000000
VERSION     DAWAEA010000103.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3596_length_3895_cov_2.150260"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2385..2394
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3621..3630
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          765 a         1157 c         1098 g          855 t
ORIGIN      
        1 agtccgaagc ccgcgccccg taggggagcg ggccgcagag cgctgttcgc gggcaatgac
       61 ggccaatcag tttttttcga cagtctgagg tgcggaaata cacgcgcctt ttctttattc
      121 aacagcgctt tgcagcccaa gtggcctcat aaagcgctac agaaattcac ctcatgaaac
      181 aggtcatccc atcccaaggc aatcacaatc cccatcatta ccgcatttca tcgctccatt
      241 gctccaacgg agtctcccac tttccctcct atcaaaaacc ccggtgcggc ttcatatccg
      301 caccggggtt ttccatgtgt tcattagata ttgcagggaa tggcgctgga ggagaacaaa
      361 atctgctgag tctgctggcc cgcaatgccg tccatttcca gtccgtgcac ctgctggaag
      421 gtgagcaccg cgttggtggt ggcggtgtcg taggtgccgg taatgtcgcc gtcgtagtac
      481 ttcagctcgt acagcgcgta ctgcagctgc tttaccttgc tgccggtgtc gccctgctgc
      541 agcttggagt agctgccgct ggcggaagtg ccgccgtaca gcaggcgctg ggtggtgggg
      601 ccggccatgc cggtgacccg taggcccatg gccttctgga agtccttcac cgcctgctca
      661 gtgccgctgc cgtagctgcc gtcataatcg ccgttgtagt agcccttggc ggacaaagcg
      721 ctctgcacag cctttacatt ggagctgttg gaaccggagg tggaggaaat ggtcgtgtaa
      781 ccattgctgg aagccttcgc gccgtagctg gccgggctgg tactgctgcc ggaagagctg
      841 ccgccgctcg agctggaacc gccgttgata gcggcataaa tagcgttctg agtcgctttg
      901 cccgcgatgc cgtccgccgt caggccgttg gcctgttgga aagcagtgac cgctgccgta
      961 gtgccggagc cataatcgcc gtccaccgag ccactgtagt agcccagcgt tttcagctgc
     1021 tgctgcaggg cgcgtaccgc gctgccgttg gtgccggggc gaatggagcc aagattggct
     1081 accacgctgc tggccttctt cgcggaggaa gaatacagct tggtcagggt cgtaggcccg
     1141 gccacaccgt catcataaat gctgttgcgc ttctggaagg ccttcacagc cgcttcggtg
     1201 gtctcgcaaa actcgccgac cgccgtgccg gtcagatagc tggaagaact ggaactactg
     1261 gaactgctgg aagacgaact gctgctgttg gtggcagagg ccttcacggc gttcttggaa
     1321 tacagcgccg tctgggtgtt cttgcccgcc ttgccgtccg ccgtcagctt gttggcccgc
     1381 tggaaagcct tcacagcttc cgccgtgccc gcgccgtact tgccgtccac cgagcccttg
     1441 tagtagccca gatccttcaa ccgctgctgc agggtcttga cctccgaacc ggaggaacct
     1501 tccttcagcg tggaggaagc ggcggcggaa gaggtcgtgg gcttgggcgt agccgtcgcg
     1561 gatgccgaag aaggccgcgg cgtggcggtg gtcaggccca cggtgatggt gccgccggag
     1621 ggcgtgctgg tagcggtcac gcctgacgca tcggtgctgc tggcgggcgg attggtggcg
     1681 ttatcatcgc caaaagtcca cgctgaccag tccatctgct gcccgctgga cgaagtagga
     1741 gcgggcgtag gcgtattggt cggggcaggg gtgtcggtaa taaccgtctg gaaatcctgc
     1801 tttccgctgt tatccagagc gttgcccgca tccaccacac ggtcaggctc catatagcag
     1861 gccgtcatca gcgcccaggc gacgatcagg atcagcgcca gcgccaagcg tttcgcggcg
     1921 gacgctcccc ctttgcggtt atcgctgcgg aattgcttca ttttatccac acctttcatt
     1981 gggcggttct gaaatgttac aatcttgtaa cgaaacagca gtggaatacc actacagtct
     2041 caatgggatt ataccgtcaa aacccaacta aatcaatacc caatattctt gtaacaatca
     2101 tagaaacgaa tgggattccg tttttcatgc aggaaagtga aatatttttg tcacatttta
     2161 gcattccagt accgtcactg actgatccag tcgaagagaa tataacgcgc attcccgcac
     2221 ggggtaaggg gtggcctttt ccagtgcctg ccggtacaat gacagctgcc ggcgataacg
     2281 gggaatcagc tcctccgcgt tttccacatg gtcagtctta taatccgcca gcacccagcc
     2341 gccgtcctcc caaaagcaca ggtcgatcac gccctgtacg atggnnnnnn nnnngttcag
     2401 tgatatgcag attgaaattc cactcccggc ggacgtttgt cgaacgcagc atccgctgac
     2461 ccagcgtcga aaggaaaaac ttttccacat gggccagagg aatcagcgcc gcttcttcgg
     2521 ccgtcagcac tccgcgtacc accagtgaag ccgtttcttc ctttatataa gcgtacagct
     2581 caccgcagtg cgctcgaacg ccgtccagcg gcagcagacc cagcagttta tgggtcagcg
     2641 tgccccgcag cgctccggcg ttttccacag gggttgtgag aaaagccggc tcctgtgcaa
     2701 tggcgctgag cagtcttggc cgctgcaccg tcaggggaat gcgtttgctt tcggcggtct
     2761 cgatctcctc ttcatctgcg gactggggct gtccctgctc catagcgcgg cacagcgccg
     2821 tcacgcccag cttcaagggc aaatgttcat attccggcaa gggggaaaga gggtcgccca
     2881 agctgggcag ctgggcatcc tgcttcgatg gccacaattt ttccaaatcg ggcacttcat
     2941 tagaggaatc ctcctctttt tccatatcct gtgaactctt ttccgcaggg atgtggaaaa
     3001 ctaccctcca aggggcactt ttatgtggaa aacctgtgga attgtcgaaa agctctggtt
     3061 gaccacaggt ttcccataag aatgatgtgg gaaactcatc gcccacctgc tggttcaatt
     3121 catcccatgg cttgatagac tggatcagcc aatccagata gcaggctgcg gaacggacag
     3181 ccgccaaatc gttgatttca aagggtttgc gaagcacgtt ttccagaatc gattctaaag
     3241 catttttttc gtccgtagac gaaccaatca tcactaaacg atttttcggc cgtgtcatcg
     3301 ccacgtacag aagacgggct ttttcggccc gttcctccgc gccgagacag agctgaatcc
     3361 cgctctgcat aagggtttgc cgctttgtgc gcttttcctc attcatatac agaagggaaa
     3421 cgcccagccg gtcgtgcagg ctgacggtcc tggtctgccg cttgcggctc agacccgtcc
     3481 ccatccccat cacatacacc gtgggaaatt ccagtccctt ggatttatgg atggtcatga
     3541 tgcgcaccac gtcctcccac gggttgacca ccgtaggcga gctgctgtcg cccacctccc
     3601 gctcccggat ggagtccaga nnnnnnnnnn tccgccgggc tgcgcgccgt agtgggcata
     3661 caagcctgtg cgcatgagtg catcccaaag atattcctga gggctcatgt gccgaaacag
     3721 gaaccgttcc tcttccagcc gggtcagcac cgcccggcag cgctcctgca tggggtcgcc
     3781 ctgcaagtcc gccgtaccct gcaccgcgtc atagaagccg ggcgcgtccg ggaagttcag
     3841 ccggatatgg gccagctcct gctcgccgaa atcgaacagc gggctgcgca tcaca
//
LOCUS       DAWAEA010000104         3878 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3616_length_3878_cov_1.705990, whole genome shotgun sequence.
ACCESSION   DAWAEA010000104 DAWAEA010000000
VERSION     DAWAEA010000104.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3616_length_3878_cov_1.705990"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1438..1447
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2735..2756
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          650 a         1255 c         1095 g          846 t
ORIGIN      
        1 gtttcccacc agcagactga agttgctgac ccggaagtac agttcctcgc ccagcagggt
       61 gcaggtgccg tcagtgcgga actccgcctg cgtgccgctg gcggactccc acgaaccaag
      121 aatcagatag gcccggcgtt tcagcttgct ctccgcgtcc cgatagccgg ttgcctcctg
      181 atagaaaggc atggcccgat agggctcgtg gttccgatac agctggtcgg cgtaggaata
      241 gcacgcctcc tgatagagct gctccagatc gcggtaggct ctgggcagat tcgtccggtc
      301 tacgtcctgc aaggcccgaa tggccgcagg atagttttct tccgcaaggg cctcccgagc
      361 tgccgcagag gcggcgctgt agagctgatc gtagcagtcg gaagcctgct gggccgcatc
      421 ttcataatcg cccagtgcgg caaaggcgtc tccggcggct ttcagctcgc ccgcgtcttt
      481 ctgagccagc gccagctggt agcggcaggc gttgatccgc tccagcgccc gttcgttatc
      541 gcccatctgt tcatacaggt cgatggcttc ctgatatttc tgttcgttct ccagccgcgc
      601 gccctcgttg aggatcattt cgttcagggt gtccacggcc tgcgtgtcgt ccagcttgcc
      661 gacgttctcc aacgcagcct gcacgtcgcc gctttcctga tagctctggg cgatcttttc
      721 ttcggtgtcc ttcgcccact ggacgctgtc ctcataatct cccaactggg taaagttttc
      781 cagcgccgca gcataatccc ccgacgcaaa ctgcgactgg gccagctggt agcggcactg
      841 atttttcagt gcctcactgt cctgatagcg gcccagcaag tccagcgttt ccagtgcggc
      901 ggcataatcg ccccggctga tggcggtctg ggccagctgg tagcgcacgt cctgaagcaa
      961 gctttcgctg tcctgatagc cattcagccc ggccagcagc tgctcggcgc tctgatagtc
     1021 gctgttttcc accgccgttt tagcctgctg ataccgacac tgctgcaaaa gctccgccgc
     1081 gtcggcgtat tgtccggcac tttccaaaag gggtgcggcc gcgtcatagg cctgctgctc
     1141 catcagctgc acggccctgt gataatcgca gatctccgcc tgtcccaggc tgtccagata
     1201 gtcggggatt ttccgaaacg cttcggacgc gccctcgtag tcctcctgct ccatccgcgc
     1261 ccggccatac tggtacaggc agtcgttgcc cttggccagc gcgtcctgat aatccgcgat
     1321 ctccaggtag cgctccccgg cagtcttcaa atcgccgtca gccagcgcct tttccgccag
     1381 ccgatagacc gccgcctgac gctgttcctc cgcatcctcc cagccggagg acagcagnnn
     1441 nnnnnnnggt acagtcggat ggcttcttca tcgttgccct cgtccgcctg cttggcggcc
     1501 agccggtaat agcactcccg gactttgtcg tccgcatcct gatagccgct cagattcata
     1561 tacagggcca gcgccacgtt ctcgttgccc gtgctcagca gttcctccgc ctgttcgtag
     1621 gcgcactgct ggcagcgttc cgggctgtcc tcatagtcgc ccaaactctg gaaagtgtcc
     1681 atggccttgt cgtacagctt gtccgccaga tagctttcgc ccagcaaata taccgtcttt
     1741 tgccgctgat cctcgctgtc ctgataaccg ttcagaccgg ccagcagctt ttccgcttcc
     1801 gtcaggttgt ccgtgccgcc cgcatcccgc aggtcgaggg ccttctgata atcacattcc
     1861 tgctgacgct ccaacgcatc ccgataatcg cccatggcgg caaaagccgt cttggcctcg
     1921 tcgtactgct tggcggtcag cttctccacc gcggcgttat agcgcagggt gggcagcacc
     1981 cacagcacgc cgaacagcac ggcggccagc gccagcacgc ccgcgacact ccagcggatg
     2041 acgcggttct tccggcggcg ctgctgacgg cgctgcttgc tttcttcttc cagcaaacgg
     2101 ctgttttcct tgcgggcctc agtcgccacg tccgccagtt tcttctcatg gacggcgatc
     2161 acctgcctca cgttttcccg gctgaagctg gcgaagaccg catcccggcc ccgttcgcag
     2221 cggcagcacc gggctgaatc cagcgaattg gcccgtccgc agacgcagac ccacgcctgc
     2281 tgctgcacct ccgggtagcc catcgcgtcc tgaccagcca cgaaacgcag ttcgtccagc
     2341 gcccggcccg gctgcaggtt gttgggctca tagttcacca gcggtgcgtt gcccttgcgc
     2401 cagatggtcg cgtcgtcaaa ccagactttt tcgatggtga cttccgcttc cttgacgttc
     2461 ttccactcgt tgggcaccat catcacactg aaccgctcgc ccggggcggc cttcaggccc
     2521 tgcatccgct cggactggcg gaacagcagc cggttgtcct gatcgtagca gctcagcgtc
     2581 agaagaaggc tgaccaccac cttatgggac agattattca gcacaaacgt acattccgaa
     2641 acgtcttccg ccggcatggc ggactgccac agttccaccg gacacgtcag atcaatcttc
     2701 atggttcacc ctcccggttt ttccattgtg gatcnnnnnn nnnnnnnnnn nnnnnngccg
     2761 gcgcccgtcc acagggtcag cgcataggct tccttgatgt tcatgcaggc agcgatttcg
     2821 ttctgcacag gagtggtatc aaaggtgaaa ccgtagatgg gcagcttctt ggaaccgccg
     2881 tagaactcgg cgaacttctg ctggaagtca gcaccctgac ccttggtggg gggcagcagg
     2941 gtcacgttgc ccacgccgtt gacccacacg ctgtagctgt tggtccgagt atcggtgaat
     3001 tcggcttcgc ccgcgtcgtt caggttgtaa tgcacgcctt ccacgccgta gcacagcagg
     3061 gtaatcacgg taggatcgga gttgagcagg ttcaggaact tcatggcttc cacgggatga
     3121 gcggagttgg cggaaatagc catcatcgcg ccctgcacgg aggtattgtc gatgtagggg
     3181 gcgtcgtagt ccatcagata ggccacctgc acgccgcggg cctcagaggt ggtcacttcg
     3241 tagccataga gggacacttc ggtggagatc aggtagttcg cggtgttctg ggcattgcgc
     3301 cacgcgtcgg tcgcagtctc gccgatggcg atagcggggt cgatgtagcc ggcctggaag
     3361 tattcgcgca tctgcttggc gaatttctca aactcagggg tggcgaactt gccgaccagc
     3421 tcggtgccgt aagcgccggg ggcggacacg tcttccttgt tgtagtagta gctcagcagc
     3481 aggttggcgt cgccgggaac gatgctcacg ccgtccacga tgcgctccgc cacagcgcct
     3541 tcaaaaatga agggatagaa ggtcttgcct tcggcttctt ccccggcctt gaccgtggcg
     3601 aagatgtcgc cccagccgta gaaaccggcg ttcttcacat cgtccagcgt gtagccgtac
     3661 ttttccagca gggtcacgtt cacgtcccag cagttcatgg tggcgaaatc cttaaagccg
     3721 gggatagcgt agatgccctt ttcgccgtcc tcgccctcgg tcatggcgcc ttccagcagc
     3781 agagcgggca gagcggcttc cacgtccttg ccgtactcgt ccagcaggtt gtcttccgga
     3841 tcgtccagac gaacgaacgc gcccttcttg gcataggg
//
LOCUS       DAWAEA010000105         3810 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3711_length_3810_cov_2.158988, whole genome shotgun sequence.
ACCESSION   DAWAEA010000105 DAWAEA010000000
VERSION     DAWAEA010000105.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3711_length_3810_cov_2.158988"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          773 a         1182 c         1150 g          705 t
ORIGIN      
        1 cgtggacgca gagatggcgg tacagggcac tggcgtgggg cgcgttgtgg atgcggtagg
       61 tataccgctt gcccgccgcg tcgaaccggg cgtgaaacgc cgccgggacg taccgccctt
      121 ccagcacgcg gatgtcggca ggcaggcagg tgttcagggc aaagggaaac ttctccggcg
      181 gaatgcggct ggcagtatcg aaatgcaccc gctgccccag cgcgtgaacg cccgcgtccg
      241 tccggctgga gccggtaacg gtcagcgtgg cctcgccagt cagcttccgc agggcttctt
      301 ccaatacctg ctgcaccgcc agcgcgttct gctggcgctg ccagcccgcg taggccgtgc
      361 cgtcatagct gacagtcaca agcacccggc gcttgccctc cggacagcag cccttgatca
      421 ggggcggaag ctgggtcata gcagattcca ccttctgcac agaatggtca gaagcagcgc
      481 cgccaccgtc accagtgagg cctgccagtc gatcttctga aaatgcagca cccggagccg
      541 ggtgcggccc tcgccgccgt gatagcagcg cgcctccatg gccatggcca gctcgcccgc
      601 ccggcggaag gcgctgacga acagcggcac cagcaggggc accatggcct tggcccgctt
      661 gatgagattg ccgctttcaa aatccgcgcc gcgggccatc tgcgccttca tgattttgtc
      721 ggcttcctcg atcagggtcg ggatgaagcg cagggcgatg gtcatcatca tggccgtcgc
      781 acagggagat gggcgaggtg gtcagcgtca gcaccgaggt gcctgctacc agaaagatca
      841 gccgcatgca gaaatgcacg gcggtaatca gcccttcccg ggtaatgcgc agaatgcccc
      901 agctgaccag caccgtctcg ccgctggaaa aaaacaggtt caacacaaac gtcaatatca
      961 gcagaaagcg caggggcttc acggttttca acagttgcgc cgctttcagg cccgacattt
     1021 tggcgcacag gatgagaaac gccagcggaa tgacgtacca caccgggttg gacacgcaga
     1081 agatcatggc gatataaacg atggtcagca ggatcttcgt ccgggggtcc agctggtgaa
     1141 ccagggattt tcccggcaaa tactgcccca gcgtaatgtc cttcagcatg ctttttcctc
     1201 cttctggaac acttcccggg gcgtgccggt cagcaccatc ttcccccggc tcatgaccac
     1261 cagccgggtc gccagcgagg aaatatcgtc catattgtgg ctgaccatga ccaccgtcag
     1321 cccgcccgcg gcgtgcagct cccggatcat gcccagaatg ctgcgccgtc cggcagggtc
     1381 cagtccggcg gtcggttcgt ccagaatcag cgttttaggc cgcatggcca gcacgcccgc
     1441 aatcgccacc cggcgcatct ggccgccgct gagttcaaag ggcgagcgct ccgcaatatc
     1501 ctcataaacg agtcccactt cctgcagggc atagcggacg agttcgtcca cctcctgctc
     1561 agaaaggccc agattcttcg ggccaaaggc tacgtccttg gcgacggtct cctcaaacag
     1621 ctggtactcc gggtactgga acaccagtcc cacccggcgg cgcacttcca gcaggctcac
     1681 gtctttttca gtaatgtcca gcccgtccac caccaccttg ccggaagtgg gcttgagcag
     1741 accgttcaaa tgctgcacca gcgtggtctt gccggagccg gtgtggccca gcagaccgat
     1801 cagctcgccg tcctcaatgg tcaggctcac gtcctccagc gccgtggcgg caaagggact
     1861 gtccgtcata taggtatggg tcagatgctc tacttggatg ggcatagtac gtctttcagc
     1921 tcctttacca tgtcgctgac ggtgagaatc ccttccggga gaggcagtcc cttgtcccgc
     1981 agggtctggg ccagttcggc catgggcggc acgtccagcc caagtgcctt gatctcctcc
     2041 acccgggaga acacctgccg gggcgtgcct tccatcgcca cggaaccgtc gttcatgact
     2101 accagccggt cggcaacggc ggcttcttcc ataaaatggg tgatccagac cacggtcatg
     2161 cctttttccc ggttcagctt gcgtacggtc gccagcacct ccgcccggcc ggaagggtcc
     2221 aacatggcgg tggcttcgtc catgatgatg agttcaggct ccatggccag cgcgcccgcg
     2281 atggccaccc gctgcttctg gccgccggaa agcatgtggg gcgcggtctc ggcaaactcg
     2341 ctcatacgca cggcgctgag ggccgcctct acccggcgaa ccatctcgtc ctgtggtacg
     2401 cccagatttt ccagcccgaa ggccacgtcc tcccgcacca cgttggccac gatctggtta
     2461 tccgggttct ggaacaccat gcccgcatgg cggcggatat tccagaccct ttcctccgtc
     2521 agggtgtcca tgccgcacac cagcacccgg ccctccgtgg gcaggaacag ggcgttcatc
     2581 agcttggcca gcgtgctctt acccgagccg ttatggccca gcaccgccac aaagctgccc
     2641 cgctccacct ccagcgtcac atggctgacg gcgggccgtg cgtcgggttc gtattgatag
     2701 ctcacgtcct ccatccgaag gggaaggacg ttcggtttct gctccatagg gcttttctcc
     2761 ttattcaggg aatcagttag gatgcgcaaa aagcgcgtct cattatagcg caggagcggg
     2821 ggaaggtcaa acatatctcc gcagctaaaa aaactttccg tcaaattccg ggcttttctt
     2881 ttgccgaaaa agggagtata ctagtcttga ttaccttgag agggggttct ttcatgacgg
     2941 aaaacggaac caccaaattt acgcccgtta tggaaggcgc acgggatgcg ggagcgatcc
     3001 tgaaccatgt ataccgggcg ctgcaggcca agggctacga tcccgtcacc cagatcgtcg
     3061 gctatctgat ctccggcgat cctacctata tcactagcta tgggcaggcc cgcagcatga
     3121 tctgccgtct ggaacgggac gagatcatgg aggagctggt tcgggtgtac ctgaaaggac
     3181 tggacggctg atgggacgcg tgatcgcttt ggatgtgggc gacgtgcgca tcggcgtggc
     3241 cgtgaccgat cccacgggaa ccatcgccca gccgctggag gtatacaagc gggtcggcta
     3301 cgggccggac tgccggtatg tacaggagct ttgccgccgt ttcgacacct cgacggtgct
     3361 gctcggactg ccgctgaata tggacggcac ccggggcgga caggcggaaa aggcacaggc
     3421 tttcggcaaa gtgctgtcgg acgccgggct ggacgtgcgc tatcaggact gacttatggc
     3481 gaatgaagaa aacaacaatc tggtgcagct gcaggatgaa aatggcaacg acctgaactt
     3541 tgaacacctg atgacgctgg agcatgaagg cagctactat gtgctgctgg aggccaccga
     3601 ggatatggat gactgcaagg aaggcgaagc catcatcctg aagatcatcc gggacgaaga
     3661 cagcgacgaa gacgtttacg ccaccatcga ggacgaggac gaattcaacg ccgtgttcga
     3721 aaagtgcatg gccgccatgg aagaagaaga cgccatggcc gacgcactgg agctggaacc
     3781 cgacgaagac gagggcgacg aggagtaagc
//
LOCUS       DAWAEA010000106         3739 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3800_length_3739_cov_2.535831, whole genome shotgun sequence.
ACCESSION   DAWAEA010000106 DAWAEA010000000
VERSION     DAWAEA010000106.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3800_length_3739_cov_2.535831"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          769 a         1043 c         1146 g          781 t
ORIGIN      
        1 ggtcgggcac ttgatgctgc ccttgggcgg gatcggctca ttttcgttga gcggtcgctt
       61 gcccggattg ccctcaagga ttttgagcgc cgtgggcttg ggctttctgc ctctggttgc
      121 catgctggtc acctcccttc gcaaaagatc atcttattaa gtcgtaagaa cagcttcata
      181 cggaagctcc cgcccatccc gaagcacgct gatgggtgcg ttcggacagg ctgctcggaa
      241 ccgctccaca atgacgctgg cgtagcgcgg gtcaagctcc atggtgtagc aaatgcgatc
      301 cgtctgctcg caggccatga gggtactgcc cgaaccgcca aaggtatcca gcacgatggc
      361 gttgggtgcg gagctgttct taatggggta tgccagcagc ggcagcggct tcatggtcgg
      421 atgcagcttg ctctgggtgg gccggtcgta tttccatacg gtggtctgct tgcggtcgga
      481 gaaccatttg tgcttgccat tgggcagcca gccgtacagc acgggttcat gctgccactg
      541 ataggggctg cgtcccagca ccatggtgtt cttgacccag atgcacacgc cgctgatatg
      601 aaagcctgct tccttgaacg ccttgcggaa gttcagccct tcggtatccg catgaaaaat
      661 ataagcggat gcgccttcgg ccagatgcgg cacgatggcc cggaacgctg acaggaggaa
      721 gtcgtagaac ttcccatcct ccatatcgtc gttctggatg gtgagcgcat ccttggtacg
      781 gcccacgatg gacacattgt acggcgggtc ggttacgacg agattcgcct tgctgccgtt
      841 catgagcagg gcgaggtttt caggctgcgt ggagtcgccg cacaccatgc ggtgacggcc
      901 caacatccaa acgtcgccat cctgcacgaa gggctgcagc tcatccgcgt cgatgtcgca
      961 gttatcgtcc ttcacgtcct tgtcatggac tttggagaac aggtcgtcca cctcggcagc
     1021 atcaaagccg gtcgcgccca gatcataccc ggaaagctga agatcggaca gaaggtcagc
     1081 cagcgccttg ggttcccatt cgccgaccgc tttattgagg gcaatgttca gggccttttc
     1141 atcctgcggg ttttcaatat gaaccactac gcagtcaatt tccttcacgc cctccgcgac
     1201 cagcacctta taccgctgat gtccgcccac gatattgcca gtgacctcgt tccagattac
     1261 gggatcgaca taaccgtagg tggtcatgct gcgcttgatc ttttcgtagg ccggatcacc
     1321 cggctttaag tccttgcgcg ggttgtattt cgccggtttc agcttttcga ccggcacctt
     1381 ttgcagcttc atatctgtgt tcatgcattc tcctttatca gcataatttc cctgatttcc
     1441 agcagaataa taagggaaat ttctcttagt aggttgacgt ttcccttatt ctatggcata
     1501 atttaaggga aaccgattgg agggtaaggc aatgagagaa tttcattacg cagcgattcg
     1561 ggagcagaag tgggattccg agatactggg cctgattgcc gccatctata aggaagctgg
     1621 aaagcaggag atgtatctta agcagcgccc ggaggagttg gaaaagctgg tagagattgc
     1681 gaagattcag agtaccgagg cttccaacgc catcgaaggc attgttacca caagcacgcg
     1741 cattcgccag ctggtggaag aaaagaccac gccacgtaac cgcgatgagc aggaaattgc
     1801 cggatatcgt gacgcactga gcgtcattca tgagaacttc gacgttatcc cgatcacccg
     1861 caattacatt cttcagctgc acaaaatcct ttacagccat atgaacaatc ctatggccgg
     1921 tcagacaaag agcgtacaga actacatcag cgccacttat ccggatgggc ataccgagac
     1981 gctgttcaca ccccttgcgc ccttcgaaac gcaggacgcg ctggatagca tctgtaatga
     2041 atacaatcgc gctataggca atctggaagc ggagccgctg attcttatcc cggttttcat
     2101 ccacgacttc ctgtgcattc atcctttcaa tgatggaaat ggccgaatga gccgtctgct
     2161 cactacactg ctgctgtacc gcagcggctt ctatgtgggc aaatatatct cattggaagc
     2221 caagattgcg aaaaacaaag atctttacta cgatgcactt cgacagtcgc agacaggctg
     2281 gcacgatggc caggaagatg cgattccctt tatcaagtat ctgctgggta tcgtcctttc
     2341 tgcctatcgc gattttgaag agcgcttttc cctcgtggag aagaaacagt ctgcgcttga
     2401 aatggtgaat caggcaacac tccataaaat cggtcgattt accaagcagg atattcggga
     2461 gttgtgccct tcactgagtg tcagctccgt cgagggtgct cttcgcaagc tggtcgcatc
     2521 cggcgaactg gcacgagaag gcagcggcaa aagcacctgc tattaccggc ttaaatagcc
     2581 attgggaata ccaccagaga tcgtccttcg gggcggtctt tttttgtttt ggggctaata
     2641 cccccacccc tgaattttgc gaaaattcac gcgagagggg gccgcggtct ccagccggaa
     2701 cagcccaagg attcgatccc cccttggggg cacagcggaa cgcagcggcg ccgggcgggt
     2761 gcggcgaaaa ggcggtggtg tgcctcggcg ggccggacgc gggcaaggcg ctgtgtcggg
     2821 cggcacagcg ggcgctttcg gcgcgggaag ccggggcgtt ccttccgggc ggcgggccgc
     2881 caacggggcg cgggcggggc ttggccgcgt ggggcccata aaggccgcgt gtcgggcgcg
     2941 acggcgcggg gcgggcacgt tgccgggcgg cgggcgcggg cggtatacgg cggctttccg
     3001 gggcggacgc ggggaaggcg taagccgggc ggcggtatac ggtgggcggg ccgggtatag
     3061 ccggggacgg cgggcctatt gccggggcgc ggcgggcggc ggcccaggac gggcagaaaa
     3121 cgctctgagc tgcgcggctt gtgtagggtt acgggccaca ggtgggaagg acggcagcgg
     3181 cgcgggcgaa aaggcccgtt tccgctatat aaggaagcgc ttttcgccgg ggcgcgggct
     3241 ttgtcgattc agagctgatt tgcccccgaa accttgtcgc tttggtcggg ttgctttttc
     3301 ggcggcccgg agtgatgaat gtgtcacgcc gggcggggcg ggcgaaaagc cccgccggaa
     3361 aacgaaacac gacacagcga aaggagcgca cccccatgaa aaaccagact ttcggcatcg
     3421 agattgaaac caccggtata ggccgggaac gcacggccaa ggccatcgcc gcgtacttcg
     3481 gcacgacggc acagtatcgc ggccgccacc tcgacgactg gcgtgtgcct atgcccgacg
     3541 ggcggcactg gacggtcgaa agcgacggct cggtcaccga ccccgacgcg gaggtggtta
     3601 gcccggtgtg ccgctacgag gacatcggga tggtacagga ggtcgtccgg gccatccggg
     3661 cggcgggcgc aaaagccgac ggttcctgcg gcatccacat ccacatcggc ctcggcgagc
     3721 acaccccgca aagcctgcg
//
LOCUS       DAWAEA010000107         3734 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3808_length_3734_cov_1.774123, whole genome shotgun sequence.
ACCESSION   DAWAEA010000107 DAWAEA010000000
VERSION     DAWAEA010000107.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3808_length_3734_cov_1.774123"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2892..2901
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          825 a         1064 c         1106 g          729 t
ORIGIN      
        1 ggaatacaca tacgccacca tcagcgtcag cacggtggtg atgacgcagc agacgctcgc
       61 cacgaacagc gtgttcagaa accagcgggg aaaattgaac agcgacgtat ccgtaaacag
      121 gcgcacgtag ttctgcagcg tcatgatgcg cggccacaga tagggcgtaa acgcgccctt
      181 ttcgccccgg aaggaggaca ggatcagcca gaacaccggc accatccaaa gaatggaaag
      241 cacggtaaga agcgtgtgca gggaaacgga gacccccagc ttgcgcaggt tctttctgcg
      301 ccgcgccggg cggtcgatgc gggtcgtttc agtcttcatt ggaaggtatc ctcctttttc
      361 acggctccgg tgtggttgta caccgtcagg gacagggtgg agacgatcat gaagatgaaa
      421 atgccgatgg tagaggccag cgcgtagtcg tgctgatcca ccgtaagttt atacagccac
      481 gtgacctgac gaacttgcgc accggccccg cgccgtcgat ctcggcgctt tcgtacaggt
      541 cggaggggat attcatcaga acgccgctga aggccatcat tgtgtaggga atgccgatcc
      601 acatattgat gaccaccacg cagacgcgcg ccagcgtggg atcctgcaaa aagggcaggg
      661 gagagtcgat ccagcccagc tctatcagcg ccacgttgac ggccccctgc ggctccagcg
      721 cccgggagat cagcagaagg ctgacgaact ggggaatggc gatggaaatg ataaacaggg
      781 tgcgccacag cttcttcagc cgcacggccc tgtgattgat gaccacggcc agcagcatcc
      841 ccagaatgta gttggtgaag gtggccagca ccgcccagac catggtccac gccagaatgt
      901 ggaagaaggt gtgggtcttg atggcattgc caaggaaaat atccgttaca ttggtcagcc
      961 ccgtccacgt gaacagcttt cccgggggct gatgattggc gtcgaagttg gtaaaggcga
     1021 tcagcaccat aaagacgatg ggcagaacag taaaaagaag cagcgtcgtc atgggcgcag
     1081 ccagcagcgt gacgtggaaa cgctcgtcca gcagggtctt tccttcctcc cggaggtgga
     1141 gaagctttac cgtgcttttc agattcagga accatacata cagaaaaata gcggtgacgg
     1201 ccagcgtcag tacgctgtag agcaggatga gcatggaatt gtcgccgggc acccgctggt
     1261 agatctggtt ggcttcatcc cagacccgca gctgcgtgtt ttcgcccaag gtgccgaaat
     1321 gggacaggta cttccagcca aagccgaaga aaaaccagaa gtacagcgcc tccgcaccca
     1381 gatacagcag tcccttgacg atctgcccga agcgaaagca gccgctgccc atcaccacca
     1441 gcgacaggcg ggtgaagaaa tctccttttt tccagaaggt tttccagaag ccgctgaatc
     1501 ggtttttcac gggaaaactt cctttccgca agaaaatctg gcgcggccga agggaatagt
     1561 ccctccggcc gcgaatggtt cgttagttct gcgcggcgac ggtctgctcc accagctgat
     1621 ccagcatggc ctgcaggtcg ctgccgttgt gcgctaccag ctccgcgccg aaagcgccgg
     1681 caggagtcca gtagccgccc agcacgtgct ggctgatagc gtaggcgctc tgcgcagcca
     1741 aagcggccag agcgggctcg gaagccacgg cttcggaagc ggccacgttc acattggagg
     1801 ggccgtagcc cagcgcctca aagcggcgca gctgagactg ctcgttggtc aggtactcgg
     1861 ccagctccat ggcctccacg ggatgagcgg tctgggtgtt cacgcccaga atcttgcagc
     1921 ccaggaagga acccatctga acctgttcgc cgccgcaggt gaaggtgggc agcttgcagg
     1981 cggcgtagtt gtcgcccagc ttttccttca cagcggaaga gatccacgtg ccgcagatgc
     2041 cggcgcagat ggcgtcgccg atggagccct gcagaagatc ctgcccgccc gcaaggaagg
     2101 cgggatgatc gcacagcgcc ttgatggctt cggcggcggc cagacccttt tcgttgttga
     2161 actggcagcc gttgcccagg aagaaggagg ccagatacca gccgttggcc acgtccatgt
     2221 tgaacttctt gcctgccttt tcggcggcgg ccagaatacc gtccagcgtc tgcacgtcct
     2281 cctcggtcag gacggacttg tcatagtaga ggaagtagcc gttgtcggcg gtcatggggt
     2341 agccgtacag cacgccgtcg taggtggcgg cgttgatggc tccggcggag ttttcggcga
     2401 tgatctgatc ccggttgcgg gtcacctcat acagcgcatc ggccttcacc agcttgataa
     2461 tctggtcgtc cgggtaggag aacacgtccg ccgctgcggc gggatcctcc agataacggg
     2521 cctgcgcgtc ggcctcgccc accacgccgt aggtgaactt ccactcgttt tcgggatgct
     2581 cggcggcaaa ggcgttgcaa agctcctcca gcagcgcctg atcgggctgg gccgaccaga
     2641 ctttcagttc gatcgtctcc gcacaggcgg ctgcgctcag gcagaccatg agaagggcca
     2701 tcagcataca aagctttttt ttcatcggtt ctttcctcca ttttcaattg atttcggtct
     2761 caccgttgct tttatcataa ggctttgacg ggaatgcgtc aacgaaaaat cacgaaagcg
     2821 ctttcgctcg aaggctcggt ggacggctgg tctcatttta tgccgcttga ggaaggaaaa
     2881 gccgtgaaac annnnnnnnn ncgcctgtaa ggagcatctg ctgcaaatcg gtagaaacaa
     2941 aaaacgccct tttcgaaaag gcgtttttcg tgggattttc aaatcttctg cattttcaat
     3001 ccgtgccggg tgcagtacca gaacagcgtg ccgtgagcga agaaaggaaa acgagcttcc
     3061 agactccatt ccgggtacag ctttttcagg atcagcgtat cgttggaaag aaacgcgata
     3121 aaggaaatgt agtgctcccg acccatttcg tggccggagg tcacgtacca ttccccgtcg
     3181 gaaagctccg catggagccg ttcctccggc gcggcggcct gtacgggcag gggagagagc
     3241 tttcgtccgc agcagaccgc gtcggcctgc tccgtggaaa acagcagatt gccacagccc
     3301 gggcaaacga agaagttcag ctttttcaaa ttgccgctgc tggaaccgtg ctcctgaagc
     3361 tccccgtaca aaagggcctt cacatccacg tccagcgccc gggccagcgt gggcagcagg
     3421 gaaatatccg gcacgccgct gccgcattcc cacttggaaa ccgtcttgtc gctgacgcag
     3481 agcttttcgg ccagctccag ctgcgtcatt ccgttcttct gccgctggga gcggatcacc
     3541 gctcctgttt tgatgggatc catttcgtcc acctcctttc ccatcatagc cgaaaagcgg
     3601 ccgccgcaca atcaacgctt cgtagataag cggaaaagta tggaagatcg tctcttgaca
     3661 ggcgtttttt tatgcggcta tactagggca aacctggcaa cggacaggga aacgatggga
     3721 cggagggata aaaa
//
LOCUS       DAWAEA010000108         3705 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3846_length_3705_cov_2.037534, whole genome shotgun sequence.
ACCESSION   DAWAEA010000108 DAWAEA010000000
VERSION     DAWAEA010000108.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3846_length_3705_cov_2.037534"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    295..418
                     /estimated_length=124
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          680 a         1040 c         1153 g          708 t
ORIGIN      
        1 ggtcagtacc gcgtccacct gataaaagaa gccgggaata tcccccgcgt gctggttttc
       61 caacgtgatt ttggcatggc tggcaaaagg catgggcagg aagcaattca tgccccgatt
      121 gggattgacg gccacgggca gggaattgac ggagcagccc cgggcaaagc cgttcaggaa
      181 gaaatcgccc agcggacact ccaccgaggg cgtttcctca ccgtcccagt acatacgcag
      241 caccagatcc cgcaggacaa aacggccttt gggcgtggcg tccgtcacgg tacannnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc
      421 tggatcgcac cgctgccggt cagctccgcc agcgtgaccg tctcacccgc ggcgatactg
      481 ggaatgcagg gcgaaccctt acggcttttg cccagagcgc tgctggcctg acaggctgcg
      541 cccttggccc cggtacggtt ttcccagttg acggatacgg aacggcccga tttcagcagg
      601 ggcagactgc ccatcaaaga agtccacatc gtttgtgcgc ctcgtttctg aaagaataaa
      661 accgtcggca gggtcgcctg cggcggcatt ctagtcattc tatctgcaca gcctaaactc
      721 ctgccgatcc aaaacccggc tatacaaact tgctttttct gcttataata aagaaggcct
      781 tgatcctgct tttttttgga aaaagaacga aaggaaccac tgctttgagt gaagaaactg
      841 ttttctccga ccggcgcaag ccgctgagcc gcaagacatt gtctcccgga cgggcgcttt
      901 gtctgggatt tttgacgctg attttggcag gcgggctgct attgacgctg cccgccgcca
      961 gcgctgacgg aacgtccttg ggccttttga agggaatgtt taccgccacg tccgccgtgt
     1021 gcgtcaccgg cctttccctg attgaggccg ggcgtgacct gagccttttc ggacagatcg
     1081 tgctgctgtg cctgatccag gtgggcgggc tgggcttcat ggccttcgcc acgctgggca
     1141 tggtgctgat cggcaggcgc atttcgctgc acagccgcat tttgttaagc gagtccatga
     1201 atcagaacgg cctgtccggc atggtgcggc tgagcctgtg gtttttcgcc atggcgctgg
     1261 caatcgagct gacgggcgcg gcgctgctgg cctcgcggct gattccccgc tacggaacgg
     1321 cccggggcct gtggctgagc ctgtttacgg cggtgagcgc cttctgcaac gcgggctttg
     1381 acctgttcgg caacggcgac agcctgcttg ccttccggca ggatcccgtg gtgctgtgga
     1441 cgctgatcgg cctgattcag ctgggcggca tgggcttcgc agtgatgctg gagctgctgc
     1501 gccaccgcaa gagtctgggt cggatgtccc tgcacaccaa gctggtgctg gccgtcaacg
     1561 gggcgctgct ggtgggcggc tcgctggccg tattcctgct ggagggacgg aatccggcca
     1621 cgctgggccg ggagggcatg accctgtggg acaaagtgac tggcagcgtt tttcaggccg
     1681 tcacctgccg cacggcgggt tttgccgccg ttagtcagga tgcgctgaac gactccacca
     1741 agctgctcag ctgcctgatg atgctggtcg gcgcgtcccc cgcgtccacc ggcggcggta
     1801 tcaagaccac cacgctggcg gcgctgctgc tgctggtgca cagcgtggtg cggggccggg
     1861 atcggatcac cgtcttccga aaggaaattt cgcaggacac cggccaccgg gccgtggcgc
     1921 tgaccttcat tgccacctcc ttcgtgctgg ccgttacctg cgccctgtcg attctggagc
     1981 gccgacacgg tgtcagcctg acgaatctgg tttttgagac ggtctccgcc gtttccacca
     2041 cgggcctgag cgcggcgggc acgggagtgc tgcgccgcag ctcacaaatt ttgctgatgc
     2101 tcctgatgta tctgggccgg gtaggcccgc tgacgctggc tacggcgctg atccgccggg
     2161 aaaaggacgg cgcgcggaac cgggtgcgct tcccagagga aaagatcatg atcgggtaag
     2221 gcagcgggct ttgagcgcaa aacgaaaaaa cgcaatcact tatataggga gaaaggactt
     2281 tcatcatgaa gaacaagcaa tatctggtgt tcggtctggg acgcttcggt tccagtatgg
     2341 cccgggctct gtgcgcccag ggacaggaag tgttggctgt ggacagcgat caggagctgg
     2401 tcaaccagat cgcgccccat gtgacgcagg ccctgcagct ggacgcgacg gacgaggagg
     2461 ccctgcgctc gctgggcgtg aagaatttcg acgcggcggt ggtggccatt gggcagaaca
     2521 cccgggacag catcctggtc agcgtgctgc tgaaagaaat gggcattccc tacctgatcg
     2581 ccaaggccaa cgacgacctg cacgccaagg tgcttcggaa gatcggcgtg gataaggtgg
     2641 tcttcccgga gcgggacatg ggcgttcggt tggctcgctc catcgtcacc ccctcggtgc
     2701 tggatctaat ggaattgagc ggcgattacc agctggcgga gatcctgctt ccgtccgggt
     2761 gggaggggga aagtctgcgc agcaccgatg cgcgccacaa attcggcgtg aatatcctct
     2821 ccgtccaccg ggaggggcat tatctggtgg cccctgcccc ggatatgccc tttcaaagcg
     2881 gggacaccct gctggtgatg ggccgccggg aagatgtgga aaagctgcgg aagtaaacga
     2941 aaatcggaat tttttccaaa aacgggaaca aaatggagtc cctccaacgt catacatata
     3001 ggaggtctgt cggtcatagg ctgataacgc ccggacaagc aggcccgaaa ggatgaaaat
     3061 cagatgaaac ctacgcctga aggatatttt gaccgccccg tgtgccagcc cgtcgatgtt
     3121 caaggagacg gcaacggcat tctgttgatg cacggcttta ccggcagccc cgcccatatg
     3181 cggatgctgg cggacgatct ggcggagcgg ggctacaccg tgcgctccat ctgcctgccg
     3241 ggccatgcct ccaccgagga ggaaatggcg aaggaaagct ggcagagctg gctgcaggcg
     3301 gccaagaccg ccgttctgga aatgaagaag acctgcaaga ccgtgacggt ctgcggcctg
     3361 agcatgggcg gcgtgctggc tctgctgctg gcagagcaga tgcaggtgga cgcgtgtatt
     3421 cccatctccg cgcctatggc cacgaagaac cgcttcctgt gcctgtcggg ggtgctgggc
     3481 aaggtgatcc cgcgcatttc gtggggcgat cacccggaac ggagcgtggc gctgaacgcg
     3541 gactacgatt tcggctacac cggcttcccc acggccaagg gcgcggacct ttaccacctg
     3601 atccggctgg cccggaagaa tctttttaat attacctgcc cggtgctggc ggtgcagtcc
     3661 gaagacgatg aaacgatctg ggaaggcagc gcgtactgca tcctg
//
LOCUS       DAWAEA010000109         3682 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3877_length_3682_cov_2.831266, whole genome shotgun sequence.
ACCESSION   DAWAEA010000109 DAWAEA010000000
VERSION     DAWAEA010000109.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3877_length_3682_cov_2.831266"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          656 a         1199 c         1074 g          753 t
ORIGIN      
        1 ggcccagaat ggcgcgggcg atgggcagga agtcctccac ccggtcggcc agctcggcgg
       61 cagtgcccac gggcagttcg ggaatgtagg ccttgcgatg gcgcagaccc agattgtagc
      121 tgcagttcag cagaccgcag tacgcgtcgc cgcggccgtc gtgcaggtcg ccgtcgccct
      181 cggcggcagc cacgtacatc acagggccat caaaccagtc ggccagcagg gtctcagggg
      241 tttcggggcc aaagttgccc aggaaaacca ccagcgcgtt cacgccgtgg gccagcacgt
      301 cgtctactgc tttgcgggcg tcgcgctcgt tttccacggt cacggggcac tcgtacagtt
      361 cggttttgca ggccttgtgc agcgcggtgc ggcggcgctc cgccagcgcc atggggaagc
      421 agctgcggct gacggcgatc aggcccagtt tgacttcggg aatgttctga atcatggttt
      481 ttctctcctt tttttgtggg ttgcaaatgg aaggtcaatc cagtcgggaa agctcggcaa
      541 aactttcctt catggcggga tagagttttt cgtacaggcg gtaataccgc tcgtactgcg
      601 cgccgcgctc cgggtcgggc tgctgggctt cgttcaggcg gatgaccttc tcgcaggcgg
      661 aaggaatgtc ctcatacacg ccgcaggcta cgcccgccag aatggccgcg cccaaggcag
      721 gcccttcccg gttggctacc gtgcgtacag gcatctggaa catatccgcc atcatctgcc
      781 gccagaaggg gctggtgccg cctcctccgc aggccagcat ggcgtcaggg gccactttca
      841 tgccccgcag cacgtccaga ttctggcgct gggagtacat cacgccctcc atcaccgccc
      901 ggagcatatc ccggcgggta tgaatggcgc tgaggcccac gaacgcgccc cgggcgttgg
      961 agtccagcag agggctgcgt tcgcccatca gatagggcag gaacagcaga cgattggctc
     1021 ccagcgggga ctgagccgct tcttcgttga gcaggacgta gggatccacg cccatttctt
     1081 tcgctgcctg gcattccgcc tgacagaagg tgtcccgata ccacttgagg gacagacccg
     1141 ccgccagtgt gcagctcatg accgtatacg cgccgggaac cgccgcgcag aaggtatgca
     1201 cccggccagc cgggtcgatc tgcacccggt cggaatgggc gaacaccacg ccggaggtgc
     1261 cgatggtggt aaaggcgcgt ccctcgacca ccacgccggt gcccacggcg gcgcaggcat
     1321 tgtcgccgcc gccggcggct accaccgtac cttcgcacag gcccgtggcc gccgcagcct
     1381 ccgccgtgac gcggcccgcc gggtcgctgc tttccagcag gggcggcagc agattcttgt
     1441 caatgtgcag cttttccaga atttcttccg accagcagcg gtgggacaca tccagcagat
     1501 tggtgccgct ggcgtcgctc atttcggcgg cgtattcgcc ggtcaggcgg tagcgcacgt
     1561 agtccttcgg cagaaggata tgggcggcct tctcataaac ctccggctcg tgcttgcgca
     1621 cccagagaat cttcccggcg gtaaagccgg tcagggccgg attggcgctc agctcgatca
     1681 gccgttcctt accgatggtc tgggtgatct catcgcattc ctcctgcgta cgtccgtcgc
     1741 accacagaat ggaacggcgc agtacgcggc cctcccgatc cagcagcaca aggccgtgca
     1801 tctggccact gatgccaacg cccttcacgt cctcgggctt cacgccgctc ttggcgatca
     1861 ctgccttcac cgtgtctcgg gcggcgttcc accagtcctc gggttcctgc tccgcccaac
     1921 cgtttttggg ctgatacagc gggtattcga tggtctcgct ggcaacggcg cggcccgttt
     1981 cttcaaacag aaccgtcttg gtgccggagg tgcccaagtc aattcccaac aggtatttca
     2041 aatttctcat cccttccgat tccgcgaaac cgttgtccat ccttggaaac ggggtttcca
     2101 gaatgttagt aaacaaagtt taataactag gctatatggt atcactttcc ccgaaggatg
     2161 tcaaggcgtt ttcccccgta tgaaaagcac cggccatggt tctccacagc cggtgttttt
     2221 caaaatcaga ttacttttcc agggttcaga atcccgttgg ggtcgaatac cttcttaata
     2281 ccccgcatga gctccacctg ctcacagcca aggctttcct ccaaaaacgc ccgtttggca
     2341 tggccgatgc cgtgttcgcc gctgacctcg ccggacaaaa gccgcgcttc ggaatacagg
     2401 cgttccatga ccttttcgac ggtttcatgc cactgctcgt ccgccacgcc gtcctgacac
     2461 acatagatat gcaggttgcc atcgcccgcg tggccgaagg aacagatgcg cacctgcatt
     2521 tctttgccga tctccacgct ctttttgaca aaggcggcga tccgatcccg gggaaccacc
     2581 acgtcgcatt cgtccatgct ttccgtgctg cccttgatgg cctccaaaaa cgcgccgcgg
     2641 gcattccaga tgctttcctt gcgctcgtcg gtgtccgcca gcagcacatc caccgcgccc
     2701 agcttcaggg ccagatcggt cagggtatcg atggagggcc gcagcgcgtc gtagctggcc
     2761 ccgtccagcc gcaccagcag atacgcgtcg gcgctggtgt cggggaactg cttgcccaga
     2821 taattttccg cgcaggcgat gacctcccgc tccataaact ccacggcggt aggctcgcag
     2881 ccgcagcgca gcacctccgg caccgcctcg atgcacgcgt ccagatcagc aaagggaatc
     2941 agcagcgaca cgttggtctt gggttccggc accagtttga cggtcagctt ggtcaggaag
     3001 ccaagcgtac cctcgctgcc caccatcagg tcgatcaggc tgtagccact ggaggttttc
     3061 acgttcttgc cgcccagatg gagcacgtcg ccgttagcca gcaccacttc catgcccagc
     3121 acatagtccc gggtcacgcc gtagcgcacg gcgcgcattc cgccggcgtt ggtcatggcg
     3181 ttgccgccca tggtggcggt cttttcgccg ggatccggcg gataaaacag ccccgtgccc
     3241 tccaggctct tggggaactc catcagcaga acgcccggtt ccacggtggc ggtcatgttc
     3301 ttcgcgtcca cttccaatac tttcttcatt ttttcagtgg aaaggacgac gccgccgtcc
     3361 ttgggcacgc agccgccgca caggccggtg ccggagccgc gaggtgtaac ggcgatgttg
     3421 cgctcgttgc agtatttcag cacggcgctg atttcttcgg tcttcagggc ctgcagcacc
     3481 gcttcggggc gatagctgcc atagatggtc atttcatcgt ggtcgtaatc cggggaaata
     3541 tcctcgccgg caaacacccg gcccggcatg accgattcaa agaaagcaag atcgttcgaa
     3601 tccacggttt tgaacggctt catgctcttt cctccttcag cttctggatc atccggggca
     3661 cgatctggta caggtcgccc ac
//
LOCUS       DAWAEA010000110         3677 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3887_length_3677_cov_2.601601, whole genome shotgun sequence.
ACCESSION   DAWAEA010000110 DAWAEA010000000
VERSION     DAWAEA010000110.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3887_length_3677_cov_2.601601"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          669 a         1200 c         1094 g          714 t
ORIGIN      
        1 ccgatggcca cgatgctgtc ggcttcggcc ttctggaagg cggccacgcc gtgctgcacg
       61 ttttcaatgg tggggttggg cttgatgtcg ctgtaaatgt cgtaggccat gccggccttg
      121 tccagaagat cggtcacctt ctgggccacg ccgaacttca acaggctggc gtcggtgcag
      181 accagcacct ttttcttgcc ccggttctga agctccggga cgatattttc gatggcaccc
      241 ttgccatgat acgaaatgct gttcaggaca atacgatctg ccatgattgc gttcctcctt
      301 tggaatcaat ggactgattg ccgaacccct gacggagttc ctaaaagaag ttttcgacct
      361 tttaggacga tttccttcct tgggattatg aagggtttgt gaactttctg gatacagcgt
      421 cccaaaacat ccatgcagcc ccgtcagctg aagaacagcg cccgaatgtc ttcctccgtc
      481 aggctgctca acgccgtttc gcccgaaatg accacccggt cgaagatggc ccgcttgcgc
      541 tggctcagat cggtgacttt ttcttcgatg gtgccctggg cgatcagctt gatcacatcc
      601 acgtccttgg tctggccgat gcggtgggcc cggtcggtgg cctgatcctc ggcggcaggg
      661 ttccaccacg ggtcgtagtg gatgaccaga tcggccccgg tcaggttgag gccggtgccg
      721 cccgctttca aggagatcag gaagaccttg ccgtcgccgc cgttgaaacg gtcgcagagc
      781 cgctgccgtt cctccgggtg ggtgctgccg tccagataca ggcaggaaac gccctcccgg
      841 ctcagccgtt tgcggatgat ctgcagcatc cccacgaact ggctgaagat cagcatccgc
      901 cgcccggaat caatggcgga atggacggtc tgcaccagca gctccagttt gccgctggtg
      961 ccctcgtagt cgggcaggaa cagcttgggg tggcagcaga cctgccgcag cttcagcagc
     1021 acgctgagca cctgcattcg cgcccggggc agggagccct cgtccagcgc ctgccccaca
     1081 tgctgccgca gggtggccag cagcgtctga tagacccgct cctgctccgg cggcatggcg
     1141 gcgtacagat actgctcgtg cttttcaggc aggtcgctga gcacctcgct cttcaggcgg
     1201 cgcatcagga agggacggat ccgctcccgc agctcctccg cccgttcgcc gctgccgtag
     1261 cggcgcagga acgcggcctg ggtgcccaga tagccgggca ggatgaagtc gaagatgctc
     1321 cacagctcgc cggtgtggtt ttccatgggc gtaccggtca gcgccacccg cacctgcgca
     1381 tccagctcct tggccgccag cgccccctga ctttgggcgt tcttgacgga ctgggcctcg
     1441 tccagcacgg cgaagcgcag gggaatgtcc ttatagtatt caacgtcccg gcgaagcagg
     1501 ggatagctgg tcaggatcac gtccaccgaa tcgtcctttt tcaggtcggc gatggtgtga
     1561 cggcgttcgt cccgctgccc ggtcaccacc cggacgatga gcccgtcgtc gaagcgcttc
     1621 atctccgcca gccagtggta ggtcagggaa gtaggggtga cgacgatgct tttttgagaa
     1681 ccgtccttct ttttggcggc ggacagggcg gcgatgacct gcagcgtctt gcccagcccc
     1741 atctcgtccg ccaaaatgcc gcccatccgg gcctcataca gcgaaaggag ccagtcgctg
     1801 ccccggcgct gatagggaga cagcttcttt tgcagcttct ggggcatata gtcctttttc
     1861 gggggctggc tcagggtttg aatggccttt tccgattcct cgtccgtctg agcgtccgcc
     1921 ccggccatgc gaagcatact gatcatgtag gcggcccggt acgcgccgaa gctcaactct
     1981 ctcgtgtctt ccggaacgtt gggttcgtcc agctgggccg cgtccagcag ctcctgcacc
     2041 acgggcgcaa ggcccatcat atcccgcacg tccagaaatt cgccggtttt cagccgcaca
     2101 tactgctgcc gctgggcaat ggcgttcagg atgggcaaaa gctccggcgc gggcttgccg
     2161 tcctccagca gggagaatac cagccgcccg ccccgcatat ggaacgaagc cttggccgag
     2221 aaccggcggg gcttgacccg ctcgaaggcc tgcgaagcgt aaatggcgca gtgccgggac
     2281 agctccgcca cgccctgcgt acagaagtac aaaatttccc gggagcggcg gaggatgatg
     2341 cggcccttgt gaaccacaaa gccgaagtcc gccagcagat ccagcaccag cccttcttcc
     2401 cggccgtccc gcagcagcat tcgtccgtcg ggaagcccct gcgccgccgg agccgcgtcc
     2461 tctccgtccc gaagggcgaa gggcagcacc acggtctccc cgtagtggaa ttccattcgg
     2521 gcttccacgt tgccgtccac cagatccaga taggccacgg gcttcaacgg ctccgacacc
     2581 agccgggccc gcagctccgg cgaaggcacc accgacccca cggcggacag cgcgggcagc
     2641 agcacggaca gcacctcctc cgcattccgg gcgggatagc ggaaggaaga accctcgccg
     2701 agcaacagcc ggcacacccg ggcctgagag gaatacagat gcgtgaccgt attgcccaac
     2761 agcacatacc gcgcgtccgg cgtgatcatc cgcagctgct ccgtaccctc ggtggacacc
     2821 cgcagctctg tgggggtcag gttgacagca aagcacaggg gcagctcctt tttccggatg
     2881 ccgctgtgca tctgcttctg ttcgccaaat tgcagctgga agggatagtt ttccagatag
     2941 cgcatgacgc tctgcaaaaa aggcccggtc agcagtacaa aacgcccatc gcattgcagc
     3001 acggaggcag cgtcgctttc ggcggctccc tccggcaaca ccgcgggctc tgattcattt
     3061 ttcagaggaa tatagctcat cagcgccgtc agaaagcgct cgtcctcccg ggaaaagcgc
     3121 atactgtcgg gccggtaggt aaacttgggg gacagggtca gctccgttcc gctgacgaag
     3181 caggccagca ggtcggcgat atttttgacg gcgtacagcc gctcctgccc ggccgacagg
     3241 cccagaccga tccgaccgtc ctcgtacagg cgcagcaccg ccagcagacg gacggtctcg
     3301 ccgccgggca tggcccggtt cagggcgctc aacatccgtt gtcccaacgc cagctcgttt
     3361 tcctgctgga aacgcttgag cgtgccgtcg ctttcggccc gcagcaaggc ggccgtcacg
     3421 tgctggcagg gctgctccgt ttcgccgcag gtgcacagcg cttcgccccg ccgggtaaaa
     3481 gtcaccgact gcacgtccgt gccgcccact tcgtagcgca gaccgttttc cacctctatg
     3541 ggctgggaga ccagtccccg gcggtaaagt ttttccccgt tctgaaaggc tgccgtatcc
     3601 accccgatgg attccaaagc aatcatccgc atcctcccaa atcaaaacca atctttttat
     3661 atttctggaa aaggttt
//
LOCUS       DAWAEA010000111         3677 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3886_length_3677_cov_2.615682, whole genome shotgun sequence.
ACCESSION   DAWAEA010000111 DAWAEA010000000
VERSION     DAWAEA010000111.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3886_length_3677_cov_2.615682"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2222..2231
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          764 a         1126 c         1145 g          632 t
ORIGIN      
        1 ctacttcatg accattcccg aagccgccag tctggtgctg caggcggcca gcattgcccg
       61 gggcggcgag ttgttcgtgc tggatatggg ccgcccggtg aagattcggg agctggccga
      121 acgcatgatc cagctctacg cgccggaaaa cgggcagaag atcgagatcg tctacaccgg
      181 cctgcgcccc ggtgaaaagc tctacgagga gctgctgctg gacggcgagg gcatttcccg
      241 aacagaaaat cggaagatct tcattgccaa accggaacag atcagtgtag acaggctgga
      301 agccatgctg gataccctgc gccagtgcat tgaggaaggc ggcgatatgc gcgcctgtct
      361 gcatgagctg gtacccacct tccgggaacc ccagcaggtc aatcagaacc acgcggaagc
      421 cgcagaaagc gtcagcgcat gagccacgaa gaactgatgc ggctggcgct ggaggaagcc
      481 gggcaggctg cccgggaggg cgaagtgccc gtgggcgcgg tgctggtccg gcagggtcag
      541 gtgatcgccc gggcccacaa cctgcgggag cagcggaacg accccaccgc ccacgcggag
      601 cttctgtgca tccgtcaggc agcagagctt ttgcacactc ggcggctgaa cgggctgacc
      661 ctttacgtca cgctggagcc ctgccccatg tgcgcagggg ccatggtaat ggcccagctg
      721 gagcgctgct tcttcgccgc ccgagatcag cggcagggct gctgtgaaag cgtgctggcg
      781 ctgacgcagg accccgcctt ttaccaccgg gtgccctgcg tgggcggcct gatggaggaa
      841 gaagcccaaa cgcttcttca agattttttt gcaaaacgcc gtacaggaga caagcatcat
      901 gaacactgag atcaaagcca ttatctccga tatggacgac accctgctga acgacgaggg
      961 caagctgtcc gactacaccg tcgccacgct gcgcgagtgc atccgccggg gcatccacgt
     1021 cattcctgcc agcggccgca cccagaccag catagccccc tacgccgctc aaatcggcgt
     1081 ggactgcccc tacatcgcct gcaacggtgc gcagcttatc tcccccgacc atcaggtgct
     1141 ggaggaagag accattcccc cggaaaccgc ccgggccatc gtccgttacc tgcagaacga
     1201 gggcttctac gtgcaggtct accgaggcga gtacttctac tacgccaaag agtgcgaccc
     1261 ttccgaaaaa taccggcatt cctccggcat cgactacccg gtgccgaaaa ttctcagcgt
     1321 caccgacccc agcgaggtgc agcgggtgat gccgctcatc aaggaggctt tccggggaca
     1381 ggcagacttc accatgtcca agcccttctt catcgaaggc atcgccccca acgtttccaa
     1441 gggcaacgcc ctgcggcggc tgacgaaacg gctggacctg accccggaga acaccatggt
     1501 cttcggcgac agcctgaacg acatcagtat gctgaatttt tcccacaaca gcgtagccat
     1561 gggcaacgcc cggacggaga ccaaacaggc cgcccgcttc gtctgccgga gcaatcagga
     1621 agacggcgtg gctcatttcg tagccgagca tgtgctcggc cttcccagca cctgacccaa
     1681 actgcaaagg aggaaccggc atgatcgcca tcgaggaatt ttgtcaggcc ctgaatctgg
     1741 aaccgctgac gcaaaccacc cggaaggaaa tggacatcaa cgtcaccgac ctgaaccggc
     1801 ccggaatgca gttctgcggc ttttatgagt tcttcgccgc cgagcggcct caggtcatcg
     1861 gcaaggtgga gatgacctat ctggaatcca tcgagcccac cgtgcgggaa aaggtgctgc
     1921 ggcgctacct gagctacgac ctgccctgca tcgtctgctg ctggggcatg accccgccca
     1981 aggagctgtt gcagatcgcc acggagcggg atattcccgt ctttatgagc cgggtgaaaa
     2041 ccaccaaatt caccctgcag gccatcatgt acctcaaccg ggtgctggcc ccccacgtga
     2101 cccgtcacgg cgtgctggtg gacgtttacg gcgtaggcgt tctcctgacc ggcgaaagcg
     2161 gcgtgggcaa gagcgaggcc gcgctggagc tggtcaagcg gggccatcag ctggcggcgg
     2221 annnnnnnnn ncggacgacg tggtggacat ctgccgggtg tccgacgacc ggctggtggg
     2281 cgaagcgccc gaaatggtgc gtcacttcat ggagattcgc ggcatcggca tcatcgatat
     2341 ccgcgccatg tacggcgttg gctccgtcat tctgtccaag tccatcgaca tggtcatcca
     2401 catggaaaag tgggactcca ccaaggagta cgaccggctg ggcctgacgg aagagacggt
     2461 cagcattctg ggcgtaaagg ttccctatca ggtcatgccc gtgaagccgg gccggaattt
     2521 ggccatcatc atcgaggtgg cggcccggaa cctgagcctg aagcgcatgg gctacagcgc
     2581 cgcccacgag ctggaccggc ggctgaatga gatgatcgcg cagaactcct cgaacgggga
     2641 cggagcggat tcataaaaca acggttattt tctaaaaata tgggcagtac gggaggatgg
     2701 aatcatggta tacattggca ttgacgtagg cggaaccagc attaagatcg gcgtggtgga
     2761 cgagcagggt cagctgatcc atcagggcga gacccccacg ctggtgggcc gcccctatca
     2821 ggagatcatc gcggacatgg gccactgcgc cctgaaggtg ctggatgagg ccaagctgac
     2881 ggtggacgac gttcactccg tgggcatcgg aattcccggc attgcggacg cgaagaccgg
     2941 caacgtcatc ttctgcacca acatgggctg gaaggacgtt cccctgcgca gcgagctgca
     3001 gaaatacatc aacaagcccg tctttattga caacgacgcc accgtggccg gctttgccga
     3061 gagcgtggcg ggcgtgagca agggcacttc ttccagcgtg ttcctgacgc tgggcaccgg
     3121 cgtgggcggc ggcatcgtca tcaacggccg tccgtggagc ggcttccacg gcgtgggcag
     3181 cgagatcggc cacattccca tggacatcgg cggcgtgccc tgcacctgcg gcaacgaagg
     3241 ctgcctggag cgctactgca gcgccacggc catcatccgt atgggcaagc agatcctgca
     3301 gcagcacccc gaaagcatga tgatggaaat ggtgggcggc gacccggaga agctcactgc
     3361 caagattgtg ttcgacgcgg cgcgggagct ggacaacgcg gccatgaagg ttttcaccac
     3421 ctatgtggac tatctggcca aggcctgcta caccatcatt gcgttcctcg atcccgaggt
     3481 catcgtgttg ggcggcggtg tgagcaaggc cggttccttc ctgctggacg cggtgcgcgc
     3541 ccgggtgccc aagtacctgc tgttcaagac ccttccctat tcccggattg aactggcccg
     3601 tctgggcgcg gacgccggca tgatcggcgc ggccatgctg ggcaggcagg cctgattttc
     3661 ccaaaacgct gaggagg
//
LOCUS       DAWAEA010000112         3664 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3899_length_3664_cov_2.267387, whole genome shotgun sequence.
ACCESSION   DAWAEA010000112 DAWAEA010000000
VERSION     DAWAEA010000112.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3899_length_3664_cov_2.267387"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1685..1694
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3116..3125
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          728 a         1087 c         1105 g          724 t
ORIGIN      
        1 aagctgagga acgaccgctc cgccgggaca atcacccggg tgcctggcgt ttccggcacg
       61 gcgatgttgg accaccagta catgtgcttc gtttcgtccg tgcgattctc gatccggcag
      121 cgcaggtaca gcgtgggcga atcctcccgc acccacgcgc tgacagagta taccacaccc
      181 cggatgcgct cgaactcata cagccgcagc acctcgccct cgggcgtttg atcgaccgcc
      241 gcaaagaccg gcgagcaggt cagcggattg tgtcccttga tgcccacgtt gaactcgacg
      301 ccgccgctga accacgcgtt gcgcaggccc agattgcccg gctgaaatac cggattgacg
      361 tacaacagtt ccttgcgctc atccttgtta tagatggaat acagccgtcc gcccagctcc
      421 ggcaggaaca ctgcgcgcac gtgatcgttt tccaccacgg cggcctgaaa cgcccggggc
      481 ttcttttccc ggtcgtagcc gtcctgcagc cggtagggca gcatggtggg aatcatgccc
      541 tgaccgatgt atttcttttc ttcctccgcc accttgtccg tcatttcata tccggcgtgg
      601 atatagctga cgttcttgat gtccggcatg gggttcagcg gcccaagcga ggccgcggga
      661 atcacgagct tttcaaagtg taccatgatc gtctcccctt cagcagcttc cattttttcg
      721 ccgaacgacc cccttcgggt cgccccggcg ctgtcattca gtttcttcac gctttccgtc
      781 ctgcttatct tatcacggcc ttctccctgc cgcaaggaaa atcattcgac tttcccggtt
      841 tttccgcagc gagcggaacg actttttcgg cgcgctgtgc taccatcgtc ttatccgaaa
      901 aaatagtccg aacctttccc ccttctcagc cgttataatg gtgtaaatgg gcccacgaaa
      961 gaaaggggag atcaaccgga atggaggaag aacgttttgt cagcgaggtc cgcgccatgg
     1021 agcgcacgct gtaccgggtg gcgcggggat atctgcgcac ctgggacgag tgcgcggatg
     1081 cggtgcagga agcgctgtgc aaggcatggg acaagcgaaa gcgcgtggac gaaagctatt
     1141 tccagccatg gctgacgcgc atcgtcatca atgaatgcca caatatcggc cgaagaaaga
     1201 agcggctgat ccccgtggcg gagccggaac gggagcccgc gccggagccc gccctgccgg
     1261 agctggcgga cgcgctggag gcgctgccgg aaaagctgcg gctgccgttg agcctgcatt
     1321 atctggaggg cttctctgtg gaggagatcg cccggatatt acgcatccct gccggaaccg
     1381 ttaaatccag actgcatcag gcccgaagcc agcttcgcgt tcaatggctg gagacggaag
     1441 gggacgaaac ggcttcccgt ccgccctatg gccgttcatg gaaaggagca gaaaaatgac
     1501 cgctgaaaag atcaagcatt ggcaagatcc ttacgagcct accccggagg gctttcatct
     1561 ccgggtggaa aacacgctgc gatccttgca gcagaaggag aatgccgctg tgccccggat
     1621 ccccgtccgc cgggcagtgg tgctggcggc gctgctgctc acgctggcgg gcgcggccta
     1681 tgcgnnnnnn nnnncgtctg ggcgtgctgg attttctgac aacccgcatc tggagcggcc
     1741 ctgcggcgga agacgtgcag ccgggcatta cacaggattt ccgcgctgaa agcgaatcgg
     1801 ctgtcctgca tctggacgca cgggatctgt atcagaatga agacaaattc tctctttgtg
     1861 tgcatatttc cccgaaggag ccggagcgct accgtctgct gtacgaaacc gatatcggcg
     1921 ccgacggcga gcatttcgac cggatctggt ggaacggaaa gcagctttcg tttgacgaat
     1981 ggctgccgga gggcaagcag atgctggtgc tttccgtccg gcagatgacc gtcggcggcg
     2041 tgaccgtgcc cgtcagcatg gactggctgc cggaggagca gggcgagacc ttcctgctgg
     2101 aagcaccgtg ggaacgggcc agaagccaga atgccaccgt gaatcctgac ggaaccgtca
     2161 cgatccggct ggagctggag accgatgttt acggaatcgg tcagccccag accagcaccc
     2221 tgaccctgca ggttccggcc ggtcaggggt ttgccaaaaa cgaaaaggag gaagccaaat
     2281 gaaagccttc aagtggattt gggtgagcct gatgattctc tgcctgctgt gccctgcttt
     2341 ttctctggca gaggcaggcg aaccccgttt ctgtctcccg gaggaagtgg atctgtcgca
     2401 ggtgcatgaa atcgacgggc gtttgaaggg agagatcctt cttccgtcct gcgaggggga
     2461 cagcggttcc tatcgtctgc tggtggactg ccccgttccg cagggctttt cggccaagca
     2521 gcaggttcgc cttcaggtgg agtatcagaa gatcgaaaag cagcagatga gcgaagcgct
     2581 gtcttctttg gggcagaacg tttccgccga tcagatgcgt tccagcagct ccggctgggc
     2641 cagccgtttc ctttactatt ccagcaccgg cgcggacatc aacggcagcg attcctattc
     2701 ccatccctat acggggaagc tctgccaaag cctgtccgcc gggcatgaag cggaaatggc
     2761 cgcggccaag gattttgccc gcgcactgct gaaaaagctg ggcgctaaag ccagcgagcc
     2821 gtttctgcag gcgctgcgcc tgacgcccga ggacgttctt tccgcctaca gcgctacgga
     2881 aagcagcagc acgtggtatg aagagattcg tcaaagcacg gaagcccggc tgtccggcaa
     2941 ggatcaggtc acgctgcttc gcgcccagta tgaactgctg gatctgcccg tcatgtttca
     3001 attttactat caggaagggg aagattcctt cggggaaagc tcctatttgg agctggtgat
     3061 gggcgacgac ggccagttga agcagctggc cctccgcagt ctgcccgtcg tgacgnnnnn
     3121 nnnnngaaga atggctccgc ctgtgggtgg ccaacgtctg gtggccctcc tcggtcgctc
     3181 aggattacga gatcaccggc gatcctctct acggcgatta taccagatac gccaccgaag
     3241 aaacgctgac ggccattctt ccctgctggg tgggaaatga aaagggctgc ctggttcccg
     3301 gctggttcac cgaatgccgc tgcaccgttc tcaaggacgg cagcacctgc ggctattccg
     3361 acgcctatat ggacgcgctg accctgaccc gaatcttttg aggatgacaa aaagcccggc
     3421 gtgcattcag cacgccgggc tttttgaaat ccgtattttg cgcccgctgg catcccgcgc
     3481 cagacctgca ggcggcggca actccacagc caagccactt tccctcgagc gttcccgcgc
     3541 ccaaagggcg cgcagtccgg ggtgcagggc ggcgagagga agcgtgcatc cagtggatgc
     3601 aaccgccgta ggcggttagc gaccggcagc cgtcaaccga aacgaggatt tggcacggca
     3661 gtgc
//
LOCUS       DAWAEA010000113         3657 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3907_length_3657_cov_1.868406, whole genome shotgun sequence.
ACCESSION   DAWAEA010000113 DAWAEA010000000
VERSION     DAWAEA010000113.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3907_length_3657_cov_1.868406"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2717..2808
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          793 a         1083 c          899 g          790 t
ORIGIN      
        1 ccagcgcagc ggcacagacc agatccatca gggggtagag ggcttccaga tcggcggtct
       61 gttccgccgc ggccatggcg cagcccagca tcatcacgag tgcaacgacc catgcaaatg
      121 tctttttcat ccactgcttc atattccgta ttcctcctaa gctatcgtgt atgaacacca
      181 cgcaggtgtc ttcctcttat aagacgaaca acccgcggga tttcatccaa gtttggcaat
      241 atattctgct gcggcgatgg tacagatggg tctcccgatc tgtggtcagg gcgattttgc
      301 tttcttcccg cgcaaggctg cggggttttt ccgctacgat ctgcacgctc tccggcaacg
      361 ccgaagcatc gtggggtttc agggcgtaat agtcgacgct ccccgccagc gtgtgcagaa
      421 acgccgcttc ctccaccccg gaggccgaag cagacagcac cgcgacccgc tttccacgat
      481 agaacatgcc gtcgcaggtg gcacaatagc tgacgccgcc gcccagcagt tcttcttcgc
      541 cgggaagaag tacgggccgg gctgcaccca tgcacaggac gacagtcctg gcttccagca
      601 cgtcgttttc cgccagcagc aggaagcaat ccccactcgg catgatctga cgcaccagcg
      661 cgtttttttc ttccgcgccc aacgccaaag cctgctgacg aaaccgttcc agcaattctt
      721 tgccggggat ctgcggcagc ccggggtaat tgtcgatccg ctctgcgccg ccgagccagc
      781 ctcccgtttc cgccggggcg atcacagcgg cgctcaagcc gcgcatccgg gccgtcatgg
      841 cgcaggacca gcctgcgggg ccattgccga caatcgctat atccacaagc ttttcctcct
      901 ttgccatttc tttggagtga actcagtata acaaatccgc cgaaaaagca caagagaaac
      961 gttataagtg taacatattt gacacaaaaa gaaacattta gaccttattc gccttctcct
     1021 tttttgaaac gctcctctac ggtgttgcgt ttccatgcga ccagagctcc ggcaaaaatg
     1081 ccgcacggat gccaactgaa ctgttcaaac ccaagctgag aaagcatttt cattgcacgg
     1141 cggcgcgtga tgttttccgt tgcttccgca ggagtttcac gggtcgcatc cagcgcgctg
     1201 cgaaggggcg caggcagatt ctccagcccc atcaccagct gtccgccttc cttcagtacc
     1261 cgatgaacct cccgaagctg attttccata gcggattcat ccaaccggcg gggtctgagc
     1321 aggacggcgt gaaaagcttg atcccgccaa gggatatccc ctgcggcggc atacaccaga
     1381 tcggcgctgc gcaccagacc acgggtggag cgaaccagtt ccatatcgtc ggctacgccg
     1441 caaacttcac attccatatt gcgcagcaga tattccgcca tcaggccgtt gcccagaccc
     1501 acgtccagca cccggtcgtc acggtcgatc actgcccatt tcagcaggca gcgttccatc
     1561 gtgctgagcc ttgggtggcg cgacggaaaa tgcgcccgtt cgtaggttct gcttcctgcc
     1621 tttgaaatca tacaaggcct ccgtccattg cagtaggggt tctccttacc gtcaaataca
     1681 gaaagaaaac cctgccggta aacacaccga cagggtcatt atatcatatt ttcttcaaaa
     1741 atgccatatt tttgtaatat tttttcaata tttaagaaac taattcatat ttttcgttca
     1801 ctgcaataaa aaagacaaaa caaaaagctg atgctcaaat gtgcatcagc ttttatactg
     1861 catcttacag gcacttactg cgccttcgcc agcctcttgg ccagacgagc ctttttgcgg
     1921 ttcgcggcat tcttatggat aacgcccttg gtaacggctt tatcgatagc ggaggtggtg
     1981 acaccgtaca tagtagcggc gttcgcaggg ttttcagcga cctccgattc aaacttctta
     2041 acggcggtct tgaccttggt tttcacgatg cgattctgaa ggttcttggt ttcgctgacc
     2101 ttcacgcgct tgatagcgga cttgatgtta ggcaacgtct ttcacctcct tggtattcat
     2161 tcagtcgcag ggtatatttt atcatgcgat ccctgaaaaa tcaatacctt ttttccaatt
     2221 ctttcgccac ctcttttcag ggcagcagca ttcctgccgc taacgccgcc acgcaggcgg
     2281 cggcagccac cgtgagcaaa cttttgccca tccagccaag caccagtgcc accgccgcgc
     2341 caatggccga agcggtcagg ctgcccgtgg cggaaaacac tgcgggaaag gtcatggctc
     2401 ccagtacggc atagggcaca tagtatagga aggatcgtac aaagggactc ttgactttgc
     2461 tgcggatcgc cgccagcggc agcatacgga tcaggtaggt cacacccgcc atcaccagca
     2521 gatatttcag aaaagccgac cactccatca gtccagcgcc tcctgttctt tctccgattc
     2581 atcctgcgac gcatcagggg gaacgggcat cagccacgct cccagcgcgg aagccgtcac
     2641 agcacagagg atgatgcgga acccgccgga aataaaggaa aagaacgaaa gaaactcaaa
     2701 catgcagctc atggcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc cagcgcatcc
     2821 gtcacgctct tgggcagaat cccgcctgcc agcgctccca gcgcggtgcc cagcacccag
     2881 ccgatgaacg gaccgttggt caggccgtac agataagcct tgcccacctt gcccggctgc
     2941 tgggacgcta ccacgaacac ctcgtccgtg ttgcagaagg acagaagcag ccggtggggc
     3001 gtggtcatgg tttcgtccag tttctggctc aggctcaagc tcatcaacgc ataacgcagg
     3061 ttgatggtca gctgggtcaa agccagttcc gtcaggcttc ctccggcggc catcaactga
     3121 aggcccgcca gctggccggc actggtcaca ttgaaaaagg agatcagaac cgcctgaatg
     3181 ggataaagtc cctccatcgt cgcctgaatg ccaaaggcaa aggctaccga aaggtagcct
     3241 aatgcaatgg gcaagccgtg acgaaggcct tttacgtagt ttttcaaaac attaccttct
     3301 tctgcgcagt ttgtttacga cggttccggc tccgataagg gtcaggatcc agccgatcag
     3361 gcccattctt ttcccgttgc cgcgacgatt tcgacgcgcc tttcgcgccg ggtcgctctg
     3421 gctgaacacg atcatcacca gaatggccag aaccagccag ccccgagcgc ttaacccgcc
     3481 ttcgtcctgc tcgtgggtct cgtgatactg ctgataatcg gcggtatttt ccgtgatgga
     3541 gccaatgacc tgctcccaca gttcagaacc agtggaggac tctttcgaag tgaccgtctc
     3601 ttccgtctgg aacagaccgt tcaggctgat atcggcgtta tactgccgtt ccagcag
//
LOCUS       DAWAEA010000114         3651 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3918_length_3651_cov_1.848721, whole genome shotgun sequence.
ACCESSION   DAWAEA010000114 DAWAEA010000000
VERSION     DAWAEA010000114.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3918_length_3651_cov_1.848721"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    334..452
                     /estimated_length=119
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1812..1821
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          528 a         1245 c          921 g          828 t
ORIGIN      
        1 gtggacatgg tgctgcgcgc cggagagggc ccgcagcatc cgcagcgcgt cctccatctg
       61 atcgcagctt tccttggtgc ccgtgtccac cagatagtcc tgcccattga gccgcagcag
      121 gatcgcatcc gccttcccca cgttcaaaaa ataagcctga atctgatcct ctgcatttcc
      181 tgccggacac gctatccaga acagcagtgc gatcagcaga caaacagccc accggcctgt
      241 tcgtttcatt ctgcatccct ccctttttat tatatccgcg agggatgctt tttgaaaaga
      301 gaggccgcgt ccatttttac aagttatttt cttnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggtctacg atcagcccca gcagggacag
      481 cacctgcaaa accgccgtca ccagctgcag caccgcgtcc tgcgtcacca ccggggcaat
      541 gtcgaagtac cccagccact ggtataccgt gctgacgatg aagctgagga acgtcgccag
      601 ccataccttg tttttcagtc ggaccgtcca gttgatcttc atgtttcctt tcctcctttt
      661 ctgccctgtc aggcaataaa cttgttttcc tcgcagcatt cccggtagat gtcccgaatg
      721 taccgatagt cctcctcgaa aacgccgttg gtttcattgg tgcgcgtcaa tagcatctcg
      781 tatttttcgt tcaggtcgat gcggtccatt tcgttgcggt cgatcttgct gtccatctca
      841 gatagctgct ggcgcagatc gccggtcagg gcgttgccga tctttcgcag cagccatacc
      901 atgggcgaga cctccatggg cttcaccttg atgcacgagg ccagcagcgg gagcagccag
      961 ccccagtgtt tttccagcca gtccaaaagc gcttccatcg gactacccct ccagatagcg
     1021 gttcatcatc cacgcccggc ggcgcacgcc gtcggtgcag atggccgtga ccagcgacca
     1081 ttcgcccttc tgccgctcca caaggacggt ctcgccgctt tgcaccttcc agtacagatt
     1141 ttctgtcttg caggcacgtt tccgaagctt gaccgggttc ccgtcgggcg tgcgcactac
     1201 cgccgaaaag gcgctctttg cgtctcccag caccggctcc gctgcgccag aaacttcgcc
     1261 ctccgttccg atctccgcgc ccagcgtgac ccccggcttg acttccgcgc cgtagtcgat
     1321 ctcctgcgct aagcccacgt gcgtccatcc gttttgcagc gtggagccgc atacccggcc
     1381 catgctctgg gagctgtgca ctacgtcgca ggccctgcgc tttccgtttt tgtccgtgtc
     1441 ggtcaggacg ttctccccca cgtacatgcc cacatgggag aagtccccga agccgtcccc
     1501 ctgataccgg gccggaaggc cgctttcgtc atcctcatgg ataaacagca gcgcccccgg
     1561 cgccagtttt ccctcggctc gtgcgttctc aagggtcccc agccacgccg cacttcgcgc
     1621 catgtggttg ctgccccggt agtccatctg gccgcccacg ttccgcacgg cctgttcgat
     1681 cagggcctga cagtccatct cctgatagct gtggtagctt tgggccagcg ccaccgccgc
     1741 cgcggcggcc atttttcctg ttggtctcat tggttccctt cctctctgaa aaaagaggga
     1801 cggatgaact cnnnnnnnnn ngcccccttt cttgcggccc gttccacgcc ttgcggcgcg
     1861 ttacgggctt cgggcctgcg gtacgttgtg tcctcggccc tcgttccgct tcgcggaacc
     1921 tttctttcgg cccgccacgc tttacgccgc aagccccggt tccccgttac tccccgtccg
     1981 ccgtgccgcc gtactccgta ggcaccagct ccggcaggcc actgtcgatc agcacttccg
     2041 ccacgccctt tttcagggcc tttggcacct gctcaaaagt cgtcttgccg agaatgaccc
     2101 gctgcgcaaa gaacatagcc atcatgatac accattcctt tccaaagatg atttttattg
     2161 cgaacagctt tacagccgtt atcgcctttg tcggcccgtt ccacgccttg cggcgcgtta
     2221 cgggcttcgg gtttgcggag cctttcaggc tgtcctcaac cctcggtgct ttgcaccctc
     2281 cttttcggcc tgcccccctt cgggtcgcag gcttcgccgc tgcggatggc ttgagcggtt
     2341 gcgcgtcccg aatcttattc gggccgtgca attccagcca aaggctggaa cagtctggcc
     2401 acgttccgtg gacagactgc ccttgcatgt cctcacggct cggtgctttg caccctccac
     2461 atttactttt catctgcgta caccaccgtc gccatttcgg cgatacagtc ctccacaaaa
     2521 tcgtttcggt cgctgatggc cttgatctgg gctttcagca tggggatgtc ctctttcaat
     2581 gtctgggctt cctgctgcgc caccgcaagg gggctgtcca gtttttcgcc gccccggtaa
     2641 aaatgctccc cgtcgtaggt gtcgccgacc gccacgggca aatccccgca gggaaccgcc
     2701 ccctcaaatt ccgccgcgtt gggcgggtat agcaccatca cgttcgttac cacgccgttt
     2761 tccaccagtg cataaaacgt ctcgttcacg tttcttcctc cttaccgatg atttcggatc
     2821 acggcaatac cgctgccgcc cttgcctacg ttcgttaggc cgtagtacat gccgcctcca
     2881 ccgccgccgg tgttggccgc gccgctggtc tgcgggttcc cgttccaggc cccgtttccg
     2941 ccgcccccgc tgccgccctt ggcctgtgcg gagccgtttc cgccagcacc gccgccgccc
     3001 gcgtacaggg tgccggtgga ttcgccaaac tcccgcgtgg tcgtcccctg ccccttgccg
     3061 ggggaacccc aatggtcgat cccgatgttg gcggcgtcct gcgggtcgcc gtttccgccg
     3121 tccgagccgc cgttgttcac ctgagtattg ccataagcgc cgccgccgct gccgccgccc
     3181 agcttcgtgc cgccgttcgc cgtcacgcca agggcgctgg tgtttccgcc aggcgcaaag
     3241 gcgctctgtc cgcccgcgcc gatcacgatg ctgtaggccg tgttcacctc cacctgaatg
     3301 ctcctctggg tcttggtgta cccgccgctg cccgccttgc cgtagccgtt gttcgcgtcc
     3361 cagctgccgc tgccgcccgc gcagcccccg ccaacgcaga atacgtccag cttgccatcc
     3421 cacttgccaa ggttggtaaa cttcagcgtg ccgctgctct tgaacttgat gcgccagttg
     3481 ccgcttccgt cgtcgatctt ctcgctggtg ccgctgtagg tgtaggtcat gttggggtct
     3541 ttcaccgtca gcgtggccac ccggctggtc acttccccgg ccttgttggt caccacacaa
     3601 taaatgctgt ggctgccgac gctggcgccg ctccagctga cggtggcgtt g
//
LOCUS       DAWAEA010000115         3647 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3925_length_3647_cov_2.861915, whole genome shotgun sequence.
ACCESSION   DAWAEA010000115 DAWAEA010000000
VERSION     DAWAEA010000115.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3925_length_3647_cov_2.861915"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          690 a         1170 c         1046 g          741 t
ORIGIN      
        1 gctcaccggc cgcgtttttt ctttcttcct ccatcgtcct aaaaaacctc ccgatgtgga
       61 ctggcccgcc aaagttgcgt gcggacaact tctattatat cgtcccccgc ctttcgatgc
      121 aaccggggtg acgccgcgcc ccctctggac ttgcggggcg catccagggt aagtggggcg
      181 ggagtggaaa tggtgatgga tgaatgttgt tgggggctta tctcgatact gccgcgaaaa
      241 gaatggacaa atgagccatt tctgcggatg atattgggat tttgccacag tttccttccg
      301 gcagacggcg tatgaactgg atctcgctat gcgtggcctt tgtcaccgaa ggaaagaact
      361 cgcttttttt cagcgttttt gattaatcat tgagaataat aaagaaaagc atgggaggaa
      421 acaaaatgaa gccagagaaa gccatggagc catctggccg ggagctgccc gggttccgca
      481 aaaagtcctt tgccctgccc tatcgcagcg ggatgatctg gttcgagcat ttggacggac
      541 tggggcgttt ttgccagggg gcggacagta aagtccgccg ggagctttcc cgacggtggg
      601 cccccttcag catggccgtc cgctttgaat cggatgtgga aaaagccaag gaatggctga
      661 ttctcgaaaa ggcataaggc tattttccgt ccaacgcgcg gcacagccgc cggtcagcct
      721 cccgttcggc cagcaggccg tcggcgtaat gggtccgaag ccccgccagc gcatctggcg
      781 tcagacgctc caccagtgcg acgagcgcat cgtctcccgc gtccagatcg gtgagcaggt
      841 ccagcaagtc ggccagcgaa tggacgtttt ccccgatcag cgccgcctgc tcctccgggg
      901 aatccgcctg aaccagccgt gccaacaggc gctcataatc ccgactgtcc atgctttcgc
      961 cgtagaacag ctccggctct gggacatttt catcggcggg cagcagaaac gcccgttccg
     1021 gctttcccag ctccagtgcg gcacggacgg cttcctccag cttgggcaga cattgggcgg
     1081 catagcgcag cgcccggccc ggcagcgcca gctgctcccc cagccggttc agcgcatttt
     1141 ccagcgccgg acggatcatc gttctgtttt cgaaatctcg ggccagcttt tccacctcgt
     1201 cgggcgttaa gtccagcgcc agcggagagc gtccgcacag cgtcagcccc agcgccgaca
     1261 ggcagaccgg ctcgaacagg ttcaccggcg cgccggcgta ttcccggctc aggcccagca
     1321 gcagccggtg taccgcccgg ggaggaaagg tgcgcagaaa ggcgttttct ccgtcgatct
     1381 cctgcagata gcgctccata aattccacgc cctgcaaggc gggccgaccc agcagaggcg
     1441 gataatccgc tgtgatgtga atttcatggg cggcaaactc cggccggtac agtcgaaaga
     1501 acccgttgac ccctccctcg atggtggaac gccagaatcc gttcggggtc tgaaacaatc
     1561 ggctcaggat cctccgctgc aaaagccgca ccgtccgcag cttttgacgc acccggcaaa
     1621 gcccttcgtc gtacagttcg ttcaatggcc gttccttcag cgctatcgtg gcctgttccg
     1681 aggcggcata gcccttcagc gccacgccaa ggacaaattg cagggaacgc agcatttccc
     1741 cggcggtctc caccggcagg gagccgcagc cctccccggc atattcggcg gccatgcggc
     1801 gcagcagtcc cggcagctgt gcctgcaaac gggcaaggtc atcctctgtc agcagcccgc
     1861 aggcaagggc ttcctccgtc agcgaagcga aataatcccc ggggcgcagc ctttgggggt
     1921 cgatcccctg tccatttgcc agagcgttgg cttccatcgt ttcttccttt ctttgcgcat
     1981 caaggaacaa agtttccatg cctgttccgc aaaattctcg ttgacagggc tgtctgcgac
     2041 gagtatactg caagagaggc cgtcctgtac agctgatccg gggaattgga ccccggcgcg
     2101 ggcatacagg ctacgggcct cggacttttt tcagccctaa gcgccggttt gaccggcgtt
     2161 ttttgtttat ctgctttggg aaaaatgccg ctcaccgcca tccgaaatcc tccgggcggc
     2221 cgccctgccg caaatgccgg ggcagctgca gcagaaagca gtcgttcatc agctccatgc
     2281 agccccggca ggggccgtcg aacgcccggc gcatctgttc cagcagccag ccgtcaggca
     2341 ggctgtccgg cagctcggca cggcagtcgt agaacagcgc cgccagttcg gccagctgct
     2401 gcccctgttc ttcgcgagtc agccacgggg aaccggcgaa aatctccacc agcttttcgg
     2461 cggcgctttt gccgaactcg atacgtcccg ttcggcgcag ggcttcgccg tgggcttggg
     2521 cgaccgcccg cgcctgagct tccgtcagcg gcagtccccc ttccgccttg cggctgactg
     2581 ccagcagacg gtcgacttca ccctcggcgt ttcccgccgt caaaccccat tccagcttca
     2641 tggctgttcc tttcgcagcg aaaagagcag ctgttccagc gcttcccact gaaattcctc
     2701 ctgcgaaatt tcaaactgct ccgccagaaa ggctccctgc cgctgaaggg tatcgttcaa
     2761 cccgctcaag gccgaccata gcaccatcgt gcagcgctcc ggatgcagag ctgcccgcac
     2821 ggtcccttcc gcctgtccct gccggatcat ctccgccagc cgctcgttga tccgctgccc
     2881 cagcgccaat atttcgccca cggtcggatc gttgccgtcc agccgctgcg gctgtacctg
     2941 ctgaacctgc gccgccgact gaggacattc ccgcaggtaa cggagaatgg cttcgcaggc
     3001 ggcaaaatac cgaatttcga aggttttccg gcccacggcg gcttccagcg ccccgtccag
     3061 ctgccgaagt ccctttcgga cgatctggcg aaggaaatcc tccttgttga catagtaggc
     3121 gtacagcgtc cgccgactgt agccggacgc tttggaaata tcgtcgatgg tggtctgttc
     3181 gaagcccttt tgctgaaaaa gctcctctgc cgccgccagc atccgctccc ggtgcagcgc
     3241 tgccacggct tccttctgat ttgcgcccac agaagtatac ctccgtatta aaaagtatac
     3301 tattgatata ccacattgaa ccgaattttg caatagaaaa accgccccgg aaaaattccg
     3361 gggcggctgt gttgattcag aaaaggagtt ccgatcaggc cttgccgttg cagcccagca
     3421 cgtccacgat cttgtggcgc accatgttgc ggatgccgtc gcgggcgtcg gtcaggtact
     3481 gacggggatc gaagtgatcc ggatgctccg cgaagtgctt gcggacttcg ccggtgaagg
     3541 ccagacgcag gtcgctgtcg atgttgatct tgcagaccgc catggtagcg gccttgcgca
     3601 gcatggcctc gggcacgccc tgagcgccgg gcatatgacc gccgtac
//
LOCUS       DAWAEA010000116         3639 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3940_length_3639_cov_2.478237, whole genome shotgun sequence.
ACCESSION   DAWAEA010000116 DAWAEA010000000
VERSION     DAWAEA010000116.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3940_length_3639_cov_2.478237"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1691..1700
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3125..3258
                     /estimated_length=134
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          705 a          980 c         1111 g          699 t
ORIGIN      
        1 gaccccgctg agatccaact gcggcaagct ctgcggcgcg gcctgctgag aaagcgacga
       61 gaccggcgaa aacggaatgc tgctgttccc ctatgaggag tggttctacc gcaagcccat
      121 tcccgatttt cccttccatc tgtcgccgga cgattctctt tacaagggcg gcaagcggct
      181 ggtctgcgaa ggcacctgcc cccgggaata tcgcccgctg gcctgtcggt tgttcccgct
      241 gcgcattcgg ctggacgcac aggaggacgg acaggcggtc aaggcagtgc cggagatcga
      301 cccccgggca tggtgctgct gccccctgcc ggaaatgggc ggcctgcggg ccatgagcgg
      361 cgaatttacc gaagccgtcc gcaaggtagg ggagcgcatg atgcgcaaca ccgatctgct
      421 ggaggcacta tacggcgaac aacgcctgat cgacgaaatg aggcgcttct gatgaaggtg
      481 atcgtcattg gctgcggcgc ggcgggcgct gctgcggcca ttgccgccgc acaggccggt
      541 catcgggtca ccgtgctgga tcggaaccga aagcccctga aaaagctggg cgtgacgggc
      601 aacggacggg gcaatctgct caaccgggga gagctccgct actacggcga ttcggatttt
      661 gccctgcggg tattggaaca tatgccccgc ccggcagtag aggagttcct gcaaaacgcg
      721 ggtgtttccc tgaccgagga ggacgaaggc cgggtctatc ccgcctgcta tcaggcctcc
      781 gcagcggtgg acgcgctgct tgcgcagatg gatgcgctgg gtgtggagcg ccgccagaac
      841 gtgcgcgtca cggcccttcg tcgggaagac agcggctttc tgttggaagg cgtggaaagc
      901 gtttacggcg aggaaaaaag caaaaagaac ggcaaggtga aaaaaggcga gctgctggaa
      961 gaacggccgg ttcgatttcg ggcggatcgg gtgatcgcgg cctttggcgg cgcagcggcc
     1021 ccggctcacg gcaccgacgg cacggcctac gggctgttga ccgagttggg gcatacgctg
     1081 catacgccct gtcccgcact ctgcccgctg gtgacggcgg ctccgatccc ggaaaagctg
     1141 gcgggccagc gcgtccgggc gaagctgact ttatgggaca gcgcgggcaa actactggat
     1201 cagcgccggg gcgagctatt atttacggcg gacggggtca gcgggatcgc cgccatgcag
     1261 ctggcgcgga acgtgcgccc cggctgcgtg ttatccatcg atctgacgga agcgctgacc
     1321 ggcgtggcgg acacggattt gggtgcgtgg ttacaggagc gtttgaaaaa actggccggt
     1381 cgcacggctg cgcaattttt caccggcgcg gcggttcctg ctctggcaga cgagattttc
     1441 cgtcaggcgg gagtcccgca gggggaaaca cgggtctccg aatggagtgc ggaggaagtt
     1501 agggcgctta gccgggcgat tcgggacttt cgactggtac tttccggggt acggggcttt
     1561 gaggcggcgc aggccacggc gggcggcatt tccgcctctg aatttgatcc tgccacgatg
     1621 gagagccgtc tgatccccgg cttatacgca acaggcgagc ttctggacgc ggacggcgac
     1681 tgtggcggct nnnnnnnnnn gtgccacggc tcgccggttt ggtgtggtga tatgctggtt
     1741 ggctgagaat ggccgccgct cggcgacaag ccttgcggcc agtggccagg ggcgaagggg
     1801 aagaataatg ccgattgacg aagaaaaaca tagcctcgga ggcaaacaaa aatgaaacaa
     1861 aagcagcgcc tatcccgatg aaaagggaag agcactgctt ttttgattca tttacagctg
     1921 ttctaccttc acccagcgcc gctccttgga cgaacgcacg caggcttcca cgaaggccgt
     1981 gccccggaag ccgtcttcga aggaagcgta gggctgaggc gaggtacgcc agctttcgtc
     2041 tgcaatggag cggtaaaacg catccacgga attgcgcagc gcatccgccc atcccatgac
     2101 gtgccctatg ggcagggtga tgtagggtct cggttccggc aggtgcgggg gttcggatag
     2161 ataacggaag taccgacgcc tttttcaccg atccgcaggc gatccggctc ttcctgccgc
     2221 cattccatgg aatagttttc acagtccacc gtcagacgca ggtcgttttt atggccgtta
     2281 gaggctttac tggtcagcag ctggccgggc gtaccatcgg cgaagcggag caggatcatg
     2341 ccggtgtcct cggttgtcat gggatgcgct gccccatccg gcccccggcg gacgggatag
     2401 tgggtggtca gctctgccat gacctccgcc actggctgcc ccagcacggc gcaggcggta
     2461 tccacccaat gaatgccgat gtcgttgatg caccgggcga taccggtatt ttccattttg
     2521 gggctccaat ccgtttcctg cgaggcgctt tcctgcaggt agcgtcccca cagcatcagg
     2581 ggacggcccg gcagaccgtg ggcgaaacgc gccttcatct cctgcacggc ggcgttcata
     2641 cggtactgat ggttcagtcc cagcgccatg gggtgctccc cggccagacc gatcagccgt
     2701 cttgcacctg cggcactttg ggccagcggt ttttcggcat aaacgtgctt tccggccagg
     2761 agggcggctt cgttgatggc ctcatgcaga aaatggggcg tacagttatg gacgacctga
     2821 atgtccgggt cagcgatcat ttgccgccag tcggtatata ctttttccac atgatagcgc
     2881 tctgcggcgg catgagccgc gttctcatcc gcgtcgcaga ctgcggcgag tcttgcgtcc
     2941 ggcgcacggc agatcgcgtc tatatgctgg ggcccgatga agcccagtcc gatgacaccc
     3001 gcgttgataa aattcatcct catccctccc gattgaaaaa ataaaacgaa atttatattt
     3061 tgattgtaac ggcaactgcg gcaaatgaac atataggaga atcaaggatt tctttgtttt
     3121 gtccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnac acttggtttg acggactttt tccctttttg tattggctgg
     3301 gcggcctgct gccgtaaaga ggcgaaaaag gaaaatttat gattttgata tacaaatgta
     3361 tggatttcca ctattgttca aatcaacgaa aagccctaca ataaacatat catcccaaga
     3421 caccatccaa atagatagga aagagggctt ttccatgttg aattatcagg tgaagatcgg
     3481 tctggcaccc atgcgccgcg acgtcactcc ccgtccgggc atctttaact gggaaaaggc
     3541 ggaagaacgc ggccacgcgg ccgtggaata catcgagaag cactttgctt ctgagaacgt
     3601 cagcttcgtt gacctcaagg gcatcaatcc cgtcgatgt
//
LOCUS       DAWAEA010000117         3638 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3942_length_3638_cov_2.501256, whole genome shotgun sequence.
ACCESSION   DAWAEA010000117 DAWAEA010000000
VERSION     DAWAEA010000117.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3942_length_3638_cov_2.501256"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2445..2454
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          727 a         1142 c         1172 g          587 t
ORIGIN      
        1 cccggggcgt gtgcgaaatg gatgaaacag gccgcttaac ctccctgacc gagcgcaccc
       61 acatcatcac cagcgccgac ggcccgctga tgaccgaaga cctgcacacc tacacccgtc
      121 tggagccaga cacagtggtc agcatgaata tgtgggcctt cccgcattcc atgatggccg
      181 cgctggaaaa gggcttcccc gtgttcctgc gggagcagct gtccaagaat cctgaaaaag
      241 ccgaatacta cctgcccatg gcggtggacg cggaaatgca ggccggacgg gccggcgtca
      301 gcgtcaaaac cacccatgac aagtggtacg gcgtgaccaa cccggaggat aagcaggacg
      361 tggtggacgc ggtggcccgc atggcggaag ccggtctgta tcctgccccc ctgtggccgg
      421 aggcataaat ggaaaaacgg agacccaaag cgggcactgt ggtgatgatc gctctgctgc
      481 tggcgctgac cggcgtatgc gtgtacctcg cgccccgcac ccgggacgct tatcaggagt
      541 accgcacgga aagcgaagcc acccccacgc ccacggcgga taacgccaac atgatggccg
      601 ttaccctcga ccccaacgcc acccccacgc caactatgct catcctcaag ctgggcacgc
      661 agggcgacga ggtgaagaag ctgcaggagc ggctgtatca gctgggctac tataccggcg
      721 agatcgacgg ccagtacggg caaggcacgg cgcaggcggt catcgccttc cagacccagc
      781 acggcattgc cggggacggt ctggccggag aggagacccg aaacctgctg tattccgacc
      841 gggcggagat gttccgtccc acgccaaccc ccacggaaac gccctcggtg ctcaccatgg
      901 gcgatcggaa tgacgcggtc aaggcgttgc aggcccggct gaaggagttg ggatatctgg
      961 acggcagcgc tgacggcgac ttcggcgggg ccaccaaggc ggcggtggtt tggttccaga
     1021 cccagaacgg cgaaaccccg gacggcatcg ccggggccaa aacgctggcg ctgctgttca
     1081 gcgacagcgc gcccaaggcg gatcggactg cgagggcgac cgcaccgccg cgcaggcgct
     1141 cattcccatg ttcgaggccg ccaaggccga cggcgtgacc ggctggcaga tcagcgcagg
     1201 ctaccgcagc gtcaagtacc agcagaagct gtttgacagg caggtggccg agtaccagaa
     1261 tcagggcctg agcgtcgcca aggccaaaag cgccgccagc aagctggtag ccgatcccgg
     1321 ggccagcgag catcacacgg gtctggcttt cgacatcacc gtggccggaa ccaccttcaa
     1381 gggcacggag cagcagaagt ggctgcagaa gcattgctgg gactacggct tcatcgtccg
     1441 ctatcaggag gacaaggaag ccatcacggg ctttctggcc gagtgttggc acatccgtta
     1501 cgtgggcgta cagcacagcg tggtcatgcg ggacaagaat ctctgtctgg aagaatatct
     1561 gggcgagacg gattgacccc tttccctatt ttgagaaaaa aagagtacga gtagtatgta
     1621 tgatctgatc gtcatcggag cagggcacgc gggatgcgag gcggcgctgg cagccgcccg
     1681 catgggcgtc agcaccctgc tccttacctt ggacatgaat tccgtggccc tgatgccctg
     1741 caatcccgct atcggcggca cgggcaaggg ccatctggtg cgggagctgg acgcgctggg
     1801 cggccagatg ggcttgtgca tcgaccgcac cttcctgcag agccggatgc tgaaccgggg
     1861 caagggcccg gcggtacaca gcctgcgggc acaggcggac aagcgccgct atcatgagga
     1921 catgatggcc accctgtttt ccaccgatca tctgaccatc cgtcaggggg aggccatgga
     1981 gctgctgacc cagaacgggg ccatcagcgg cgtgcgtacc atgaccggcg aggaaattcc
     2041 ctgccgggcg gtggtcatct gcagcggcgt atatctgaaa agccgcatca tcatcggtca
     2101 gtattccaaa aacagcgggc cgcaggggct tctgcgggcg gaggggcttt cccagtccct
     2161 gaccgatctg ggtttcgagc tgcgccgttt caagaccggc acgcccgccc gggtggacgt
     2221 tcgctccatc gactttgaca agatggagcc ccagcccggc gacgacccca ttgtgccgtt
     2281 ttccttcctc acggtggccc agcagccgga catcggggtg aagtatccct tggaaaaccg
     2341 ggccatgtgc tatctgacct acaccaacga ggagactcac cgcatcattc gggagaatct
     2401 gcatctgtcc cccatggtgc ggggggagat caagggcacc ggggnnnnnn nnnncgttgg
     2461 cccgcgctac tgcccgagca ttgaagataa gattatgcgc ttctccgata aagaccgcca
     2521 ccagctcttt ctggagccgg agggcctttc cagcccggaa tggtacgtgc agggcatgtc
     2581 ctccagtctg ccggattttg tgcagtggca gatgtaccgc tccatccgcg gtttggagcg
     2641 ggcggtgctg gtacggttgg cctatgccat tgaatacgac tgcatcgact cccgggagct
     2701 aaacctgacg ctgggcagcc gccgagtccc cggcctgtat tttgccgggc agatcaacgg
     2761 cacctcgggc tacgaggaag cggcggcgca ggggctattg gcgggcatca acgcggcctg
     2821 ctggctgcag ggcaaggaac ctctcatcat tacccgggac atgggctatc tgggcgtgat
     2881 ggtggacgat ttggtggtga agggcaccga cgagccctac cgcatgatga ccagccgctc
     2941 cgaatatcgg ctgaccctgc ggcaggacaa cgccgacctg cgcctgacgg ccttgagtca
     3001 caccagaggt ctggcctccg accggcgcat ggcgctgatg gagcgcaaaa aagcccagac
     3061 ggcagagctt ctgcaaaagc tgcatacggt caagctgcct tccggcgaaa agcgggatag
     3121 ctggctggag cagcacggac agcccgtgac ccctgccacc ttatcggcgg aggacttact
     3181 gcgccgtccc gccattgact tggcggcgct gggggagcta tctcccgaat tggtcggtta
     3241 tgcgcctgac gctgcgctgg aggccgagct ttccatcaag tacgagggct atctgaaaaa
     3301 gcagcaggag cagatcgccc gggcccgggc catggaggac tgggtcattc cggacgacat
     3361 cgactacaac gccatcgaaa gtctccgcat cgaggcccgg cagaagctca gcgccatgcg
     3421 tccccgctcc ctttcgcagg cgggccgcat ccccggcgtg aaccccgccg acgtcgccgt
     3481 cctcatggtc tacctccggc ggggctgagc cccgcaccgc ggactgcgcg ctgcgggcgc
     3541 gcaggggcag tgcccctgca ccccacaccg cgcccatagg gcgcgggagc attcgaagga
     3601 catgaggctt ggccgggatt ttaccgccgt ggcacgac
//
LOCUS       DAWAEA010000118         3605 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_3978_length_3605_cov_2.825915, whole genome shotgun sequence.
ACCESSION   DAWAEA010000118 DAWAEA010000000
VERSION     DAWAEA010000118.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3978_length_3605_cov_2.825915"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2857..2866
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          706 a         1127 c         1079 g          683 t
ORIGIN      
        1 aatgaagaca gcgatgcaga aactgcccgc agcagtacgg aaaaattccg atgccactaa
       61 taaagcgggt gcatcctgta acattcagcc tgcgcctttt ccaattctgg aaactatgct
      121 ataataagtt gttaagcaga cttttcagct gccgcaaagg agggtcttcg gatggaacgt
      181 tccgcttctc agccgctggt gctggcctcg gcttccccca gacgactgga actggtgtcc
      241 ctgctgggca ttccctttga agtgatccct tcccgggcgg aagaaaacga cgtgaccggc
      301 tccggcaagg agcgggtgcg ggtgctggcg cggcgcaagg gcgaggaagt ggccgcccgt
      361 ctgccggggc ggaccgttct ggcggcggat acgctggtct gtgtggaaaa cgaggtgctg
      421 ggaaagcctg tagacgaagc cgacgcagcg cggatgatcc gtttgctcag cggcagaagt
      481 cactgggtgt ataccggcgt ttgtctgctt ttgccggacg ggactgtccg ggaggaggtc
      541 tgcggcacag aggtctgctt cgccgagctg gacgaagacg agatcctgcg ttacgccgcc
      601 tccggcgagc cgctggataa ggccggtgcc tatgcggtgc agggccgggc gggtgcgttc
      661 gtccgctcca ttcacggccg ccccaccaac gtgatcggtc tgccgctgga gaccgtggcg
      721 cggatgctgc gggaagcagg ctggaaattg ctgggctaaa aaacgacttt catactgaca
      781 atagattgga tgtgattccg tgggctttgt agcgcacgat ctgggcgttg atctgggtac
      841 cagcaatacg ctggtctacg tccgtcataa aggcatcgtc atcaacgaac ccaccatcgt
      901 ggtggtggac tccaccaacg agcgcaaggt gcgcgccgtg ggcgacgacg cgaaaatcat
      961 gctgggccgc accaccgacg gcgtgatggc cgtccgtccc atgcgggaag gcgtcatcac
     1021 cgactttgac atgacaggca ttctcataca gttctttatg cgcaaggcca tcggctccag
     1081 ctatctgctc cggccccggc tgtttttaag ctatccctgc tccatttccg ccatcgagcg
     1141 ccgggccgtg ggtgaagccg ccaagtacgc cggggcccgc aaggtctttc tggtggaaaa
     1201 gcccttcgct gcggcgctgg gctgcggtct gcccgtattt gagcccaacg gctgcatggt
     1261 ggtggacatc ggcggcggca ccaccgacgt ggccgtggtc tcgctgggcg gcgtggtcat
     1321 cagccactcc atccgcgtgg gcggcgtcaa aatggacgaa gccatcatca actacatcaa
     1381 gcgggaattt aacattatga tcggcgaccg cactgccgag gaggtcaagc tggatctggg
     1441 ctccgccctg cccctgcacg gcgagcgccg ggcccgtatc cggggccggg acatggtcac
     1501 caacctgccc cagaccacag agatcacctc cacccaaatc tatgaagcca ttcaggaacc
     1561 ctgtcaggcc atcctgcggg ccatcaaatg ggtattggag cgcaccccgc cggaattggc
     1621 ggcggacgtg atgcaccacg gcatctacct gaccggcggc ggctccctgc tctacggcat
     1681 ggatcagatg atcgccagcg agctgggtat cccggtgctc ctgtccaagg agcctacgga
     1741 ctgcgcggcg ctgggccttg gcaacatcat tgaaaactac gaccttctgc agcatctggg
     1801 ccgcaccagc ttcctgcatg aattctgacg gggcgtttga cccttctgac tttgctcaat
     1861 acccggaggt gaacccatga agctgaaggt acccaaaaaa gagcggcgca agagcggcga
     1921 cagccgaggc aaacacattc tgcgcaatgt gatgatcgtg ctgtgcgtgc tggcgctcat
     1981 ggggctggcg ggcactcacc tgctcaagct catcaccggc aacgaaatgc tggccatccc
     2041 ggaaaacggc gtcgccgccg tcattacgcc cctgcaaagc ggctttgctt ccgtggtcaa
     2101 ctggatcagc gattattttt acaccttgaa actgcgctcc cggctggaat tggagtataa
     2161 taatctccgg caggagaatg agcagctcac ctatcaggcg atgctcgcgg aggaattaca
     2221 gcacaagctc agcgtctacg agaacctgtt tgacgaggtt tccgacaacg aacagctcaa
     2281 tccgctggtg gccacggtca tcggccggga aagcggcaac tacttttccg ttttcaccat
     2341 caacaagggc acccgggacg gcattcagga ttacatggcc gtcaccatga acggtgctct
     2401 ggtgggctat acctacaaca ccaaaactac cagcgcttcc gtgcgtacca tcatcgacag
     2461 cgaggccagc atcgccggtc tgatctccac ttcccgagat cagggcacgg tgcggggcac
     2521 gctgggcatc gacggtcagc ccatgtgccg catgtactac ctgccggagg aaaacctgcc
     2581 ccgccccggc gatcaggtgg tcacctccgg cgtcggcatg tccttcccca agggcatccc
     2641 catcggcacc gtgcgggaaa gcacccgcgg catggagagc aacaagcagt acatcgtggt
     2701 ggagccttcc gccgatttcc agcacatcga gtacgtcatc gtcctgcgct accagccgga
     2761 ggccgaagcc gtggaggatc gttcctccag caccgactcc ctgacgctgg tgccgctgga
     2821 gaccatcctg cctgtgccca cggtgctgat cggcagnnnn nnnnnnccac cgggacggcc
     2881 tctacgcccg ggccctcctc cagcggcggc gacagctatg aatacaaggt gccctccggc
     2941 ctgcaaaacg gcgcgacgga taacagctac ggcttcacgc tggcgcccac cgccacgccc
     3001 acgccgaccg ctcccccgct ggatctgtcg gtagaggagg actgaccgtg tttaccaaag
     3061 ccctgaaagt cgccgttctg acgctgctgg cctacctttt gcaggctacg gcggcggaat
     3121 acctttccat cgcggacgta gcccccaatc tggcgctggc gatcatcgcc atcgccaccg
     3181 tggcgctggg ccgcaagtac accttcgtca tggctctggc cgtgggctac ctgatcgaga
     3241 tcatgacccc catgctggat tacatcagcc tgatcctgta ccccgtagcg gccatgctca
     3301 gcgcgctggt cttctccgac aagagtgagc gcaagctgga agaagaacgc acgctgggcc
     3361 ggcgaaccaa ccagtggaac cctcacctgc gcacccccct gtgcgcggcc ctcagtgtga
     3421 cggtctttga ggccgtccat ctgctttaca cctacttaag cggcgtttcg ctggacgggg
     3481 gccactggcg gcgcgccctg atcgacgtac tgtactccac gtttctggcc ggggtgctgc
     3541 aattccccgt gcgctggtgg ctgggggtct ataagctgaa aaaggcccga taagccgcac
     3601 tttcc
//
LOCUS       DAWAEA010000119         3576 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4009_length_3576_cov_1.798353, whole genome shotgun sequence.
ACCESSION   DAWAEA010000119 DAWAEA010000000
VERSION     DAWAEA010000119.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4009_length_3576_cov_1.798353"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          731 a         1030 c         1166 g          649 t
ORIGIN      
        1 ctgaagtaca gtgcgtctcc gaagcccagc cgggcggcga tctcactcac gccgagggtg
       61 gtggtacgca gcaggcgaca ggcctcccgc atttgcagat cgtagtgata ttgcaggggg
      121 ctcatgcccg tgtttttccg aaaaagcgct gccagatgct tgtagctgag ataaaagcgg
      181 gcttccaacg tctcggaacg gaaggcctcg ccgcagtgct cctgcaaata ccgggcgagc
      241 tcgtccgcaa gggaaggcgg ctcctccgcg gcgcttgact cccaaagggc gcaggccgtc
      301 agcagctcgt gaaggcgcag cgggtcgttc cacgggtctg tgctgcggcc gtcctcgaag
      361 cggccgacat aatcccggat cattcgttcc agccgggaac cggccagccc ggtcagcttt
      421 ttgggcagag ccagcgcccc gatctgcccc gtccggcggt cagatgggtc aaaaggcggt
      481 tcgccgcagg ggggcagttc gaagtggagg tagtaccatg aggttcccgc cggaatctgc
      541 cgctgccccc agtgatgcac cccgcttttc agaaacagca gctcgcccgg ctggacatcg
      601 tagggcacgc cgtcctcggt cacgtagatc gtgccgctgg taacgtagat cagcacatga
      661 aaaggggcga tccgatccac atgcacaaag ggcgaggcga tcaccagata ttctgccgcc
      721 cggagccgcg gcagctcccg gttgctgaaa agaatttcgt tcatcggtaa aatctccatg
      781 tttataggga aaaatgccat gttttatccc tttcggcagg atgataatac aggtacggga
      841 aaatgtcaat gcagacaaag gagagagcaa aatgaaggta cagcggcaga tcgaccccaa
      901 aagctatcaa agcgccaacg gatttattta tcattaccag cagctggtga aggacgtggt
      961 gatcccctac cagtatcagg tgctgaacga tcaggtggag ggtgtggaaa aaagccacgt
     1021 ggtcgccaac ttccgcaacg ccggacggat gctccggggc gaggatccgg gggacggctt
     1081 ctatggcatg atctttcagg acagcgacgc ggccaagtgg ctggaagcgg tggccagcgc
     1141 cctgacccgc ttcccggacg cacagctgga aaaagaagcc gacgagctga tcgagctgat
     1201 cgcccaggcg caggacaagg acggctatct ggacacccgg ttcacgctga aggatcggga
     1261 caagcgctgg acgaacctgt gcgaggggca cgagctgtac tgcatgggcc atatgatgga
     1321 ggccgccgcg gcgtactttg aggccacggg caagcggacg ctgctggacg taatgattcg
     1381 caacgcggaa tacctgtatc agcggttcgt ggtggagggc cagccgggtt acccgggcca
     1441 cccggaagtg gaactggcgc tgctgaagct gtaccgcgtc accggcaacg agcactgcct
     1501 gaagctggcg gagcatttta tcgacgtgcg cggcgtggac agccacttct atcaggagga
     1561 agcgaagaag cgggactgga cggagtggca tatgaacgtg gaggacaccc agtatatgca
     1621 gtgcgataag cccgtccggg agctgagcga cgcgacgggg cacagcgtcc gcgccatgta
     1681 tctgtacacc gccatggccg acctggcctc tcagactgaa gacccggcgc tgaaagccgc
     1741 ttgccgccgc ctgtgggaaa gcacgaccca tcggcggatg tatgtcaccg gcggtatcgg
     1801 ctccacctgt cagggcgagg ccttcaccgt ggattacgat ctgcccaacg acacggtgct
     1861 ggcggggatg cagctggacg gcaagcgttt cttctatgtg aacccgctgg aagttatccc
     1921 cggcgtgtcc ggcaaggcgg ttacccacct gcacgacctg cctgtgcgtc ccggctggta
     1981 cacctgcgcc tgctgcccgc ccaatgtggc gcggctgctg agttccttcg gtcagtatgc
     2041 ctacggccag aacgagacca ctgcgttctg ccacctgtac gccgagggcg aggtgcggtt
     2101 tgacaatggg ctgcggcttc agtgcgagac cggctatccc tatgagatgg aagtgcgctt
     2161 ccgggcgctg gaaggcaagg gacagctggc cgtccgcttg ccgggctgga gccgccatac
     2221 gacgctggta tggaacggcg cgcctgccga gtatgagacc cgcaagggct atgcctatgt
     2281 ggagcttcag cccggcgacg agctggtgct tcggctggac ggcagcgttc atcaggtcta
     2341 tgccaacccc aaggtctttg aggacacggg ctgcacggca ttgcagcggg ggccgctggt
     2401 atactgcttc gaagaagtgg acaacggcga cgtgcgtcgc ctctctctgc gcccggagag
     2461 ccagtggaag gcccttccgt tggctccggg gctgctgggc ggcattgttc ccctgcaggg
     2521 cgaaggactc cgtacagcgg ccatggaggg cctgtacgcc tatgagcggc ctgcccgcac
     2581 cccggcgacc ctgaccgccg tgccctacta tacctggggt aaccggggcg aaggcagcat
     2641 gagagtctgg ctgccggaag cataaaaaaa ctaatcaaaa cgcccttccg cgggaaatgc
     2701 ggaagggcgt tcatgcgtaa aaaaagaagg tgcgccgggg ctttaggccg gcttctgtcg
     2761 caacactttc cccatgggcc agcttataag gggcgcagcc ccgagaaggg aggacagtgg
     2821 caaagccgcc ctgcaacgcc gaagcccatc ccccgcaggg caggctgcga agcaatgctt
     2881 acgggggctg gcagcctttt ggacaaatga aaccgccctt ccgctgaaaa tgcggaaggg
     2941 cggtttattt gaaaagaaag atcagatggt gtgcttgact cgctcggatt cttcagcacg
     3001 cttggcgttg ttgaagcgat ccagattgcc caccagatag ccggtgatgc ggcggatgcg
     3061 ctcaaaggca tgaccatcct cgtggcggcc gcacttggga cagctgtcgc cgatgatgcc
     3121 gttgtagccg cagacggggt cgcggtccac cgggtggttg atggagccat aaccgatgcc
     3181 ctgatcgtgc atgtagcgga cgaccttttc aaatgcgtcc agattcttgg tggggtcgcc
     3241 gtccatctcg atgtaggaga tgtgacccgc gttcgtcagc gcgtgatagg gagcctcgat
     3301 ggcgatcttc tcaaaggcgc tgatgggata gtacaccggc acatggaagg agttggtgta
     3361 gtactcccga tcggtcacgc cggggatgat gccatacttt tccttgtcga tgcggacaaa
     3421 acggccgctc agaccctcgg cgggggtggc cagacaggtg tagttcagct tctcccgctg
     3481 gctgcactcg tcgcagaact tgcggatgaa gcccacgatc tccaagccca gattctggct
     3541 ggcgggatcc tcgccgtgat gcttaccgcg cagggc
//
LOCUS       DAWAEA010000120         3572 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4018_length_3572_cov_1.797839, whole genome shotgun sequence.
ACCESSION   DAWAEA010000120 DAWAEA010000000
VERSION     DAWAEA010000120.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4018_length_3572_cov_1.797839"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    985..994
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2423..2503
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2742..2751
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          714 a         1110 c          988 g          659 t
ORIGIN      
        1 cctccggatt gaacagctgg cccacttcga tggcgcgtct ggcctcggca tagctttcgc
       61 ccagcagagc aaggttattc ttgctttcgc caacgcccgc cgccatattc agagcggctt
      121 cttccatcag ggtctcctgc acggccagca cgtactgttc cagctccgcc gtgccgtcga
      181 tgccctccat atccttaata agggccgctt cccgacggtt catttccacc agcacgtccg
      241 tttcgcccag cggcagcaac tcgcccaacg tactgtaggc gctggccttg tcggtatcgt
      301 ccagctggaa cacaatgacg cagcgattca tttccaatgg aattttatgc tccgccgccc
      361 gagccgccag ctccgcgcca gtcagctcct gccgcagcgc cttgcggtat acatcggaat
      421 agccggaaat ggaaagactg ctcttgagca ggctcatgac catagcgtcc gcaaggcaca
      481 gcacgtcgcc cgcgccgggc gcagaggcct gcgccgccag atacagcgcg ggatgaatat
      541 ccagaacccg caccagcgcg tcgcccacat gatggttgat cccggaggcc aaagcagaag
      601 cgggcacggc aattcccggg ccgtcctccg gcacgatgca ggctccgtcc gcgtccaaca
      661 gacgcagggg aagactcaat tcctgaaaaa ctttggcgat tttgcgaaga taccgctttt
      721 caatatacac gcgaggcttg ctcctttcct gtcccggcca gcgccggaag gccaaaaggc
      781 cgcatccatt atagcggatt ccgacaaaaa agtacagtca aaaccctttg ggaaagaagc
      841 ggaaaagttg taaaaacaaa ggaagaccga aggaaacaaa gacgaagtta taccgggtaa
      901 tcgtccaggc gctcgatatc tgcgcccagc agtcgcagct tttcaggcag attctggtag
      961 ccacgctcga tatgtcccga ggggnnnnnn nnnnagcagc gtttccccct ccgccatcag
     1021 cccggccagc atcagcgccg cgcccgcccg gagatcggta ctgcgcaggc tggttccccg
     1081 cagcggatag ccgccctcca ccagcgccac gttgttctcc acccggatac gcccgcccag
     1141 acggcagagt tcagccacgt gcatgaagcg gttctcgaac acggtttcgt ggatcatgct
     1201 ttgcccgtgg cagtgcaagg ccaccacggt catcggggcc tgcatatcgg tgggaaagcc
     1261 cggataggct aacgtacgca cctcgaaggg cgagactgcc gaaccgctga gccggatgcc
     1321 cgcgtgggtc tcccgaatgc caacgcccgt ctccgccagc ttgaacagca gagagcgcag
     1381 atgctccgca caggctcccc gcaccagaac gctgccctcg gtgacggcgg cggcgcacag
     1441 cagcgtcccc gcctcgatgc ggtcgggaat gggccggtat accgccccgt gaagccgctc
     1501 cacccccaaa atggtgatgg tctcggtgcc cgccccagaa atgctggccc ccatcaggtt
     1561 caggcagttg gccagatcga cgatctccgg ctccttggcc gcgttttcca gcaccgtcag
     1621 tccccgggcc agtgcggcgg ccatcatcag gttctctgtg gcgcccacgc tgggaaggtc
     1681 cagataaatt cgcgccccgg aaagccgtcc cgacagctgc cgcttgccgc ccagcggatg
     1741 cacctgcgcg cccaaggcct gcaagccctt caaatgaagg tcgatgggcc gctgcccgat
     1801 ggcgcagcct ccgggcaatc ccacacacgc actgccgctg cgggccagaa tggggccgag
     1861 gatcagaatg ctggcccgaa ggcggcgcat gttttcctca tggagcgggc tttggggctg
     1921 cagggcgcag acggagacct ccgttccgct gcgtgtcacc tgcgcgccgc aatcccgcaa
     1981 gatggtcaga agcgtttcca catccgtgat gcgtggcact tccttcaata ccagtggttc
     2041 ctccgtcagc aatcccgcgg ccagcagcgg cagcaccgcg tttttggcgg cgtgcacctc
     2101 cacttcgccg ctcagcaccg ggctgtgacg gatgagataa gttgccatgt tcatccctcc
     2161 tgtcgcggtc attttgaccc cggcgcaaaa gccgcatgag gggtggaaag gtttttcagc
     2221 cgccccgaaa agcttttttc agccgttctt cggcaggctt tgtcagcata gcgtccgttt
     2281 tgcgcctgca gccgtccctt ggctggcggc atttgtctcg cccgttcttt tccgaaacgg
     2341 gaaagcgtgc tataataaag ccagtttttg gaaaggatca ggttccgatg gatcgatttg
     2401 tcttgaaggc tccctacccc gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntctggcc cggggcgtgt
     2521 tggagggcaa aaaaaatcag gtgctgctgg gcgtgaccgg cagcggcaag accttcacca
     2581 tggcctccgt catcgaacgg gtacagaagc ccacgctggt gctggctcat aacaagacgc
     2641 tggctgccca gctttgcagc gaactgcggg ctttttttcc ggacagcgcg gtagagtatt
     2701 tcgtctccta ctacgactat taccagccgg aggcctacat tnnnnnnnnn ngagatcgac
     2761 cgtctgcgcc acagcgccac cgccgcggtg cgcgaacgca aggatgttat catcgtcgcc
     2821 agcgtcagct gcatttattc catgggcgac cccagcgaat atgaaggcat gatggtcagt
     2881 atgcgccccg gaatgcagct gagccgcaat cagctcattg cccgactggt ggacatccag
     2941 tacgagcgca acgatatgga cttttcccgc ggcagcttcc gcgtcaaggg cgatgtgttg
     3001 gagatcattc ccgccaacga gcgggaacac gccctgcgca tcgaattttt cggcgacgag
     3061 atcgaacgca ttcgggagat cgatgcggtc agcggcaacg ctatcagcac gttggagcac
     3121 gccgcggtct tccctgccac tcattatgcg atggacaagg aaaagatgct gcaaaacctc
     3181 agccacatcg aagaagatct ggcggtgcgg gtgaaggaac tggaggacga gaaccagccc
     3241 ctttacgccc tgcggctgaa gcagcgcacc aattacgatc tggaaatgat gcggcagatc
     3301 ggctactgtc agggcattga aaactacagc cgttatttcg acggacgcaa gcccggcgac
     3361 gcgccttact gcctgctgga ctatttcccc aaggattacc tgatgattat ctacgaaagc
     3421 cacgtgaccc tgccgcaggt gcgggccatg tacaatggcg atcacatgcg caagaaaagt
     3481 ctggtggact acggcttccg tctgccctcc gcctacgata accggccgct gctgttcgac
     3541 gaatttaacg agcggctcaa tcaggtcatc ta
//
LOCUS       DAWAEA010000121         3558 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4038_length_3558_cov_2.316015, whole genome shotgun sequence.
ACCESSION   DAWAEA010000121 DAWAEA010000000
VERSION     DAWAEA010000121.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4038_length_3558_cov_2.316015"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    355..421
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2935..3024
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          632 a         1000 c         1058 g          711 t
ORIGIN      
        1 ccccgccgat ccgcagaccc ttgagcccgg cgttgtcgat cagcgtcccg gcaaactgac
       61 cttcgggccg cttgaaggtg ctgccgcagc cggggacgga caggggctgc ttttcccggc
      121 gacgggcggc gaactcccga atggtggcgc ggatggtctc cagatcgccg gggtgcaggg
      181 caaccgttac cgacagaatg gcgctgggca cggcttcgtc cgaaaaacgg gaatggcggt
      241 agctcatttg cagctcggcc ggggtatagg ccacggtctt gccgctctgc tccatggctg
      301 tcaccagcgt gaccacgtct ttcagctctc cgccgtaagc gcccgcgttc atatnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 ngcccacgga tgccgccgtc ctttaccagc aggttggagc cgttgccgat cagcgtgacc
      481 ggcacgtcca gctcccgagc cgcctgaaac gcggcgctga gctgctccgg tccgtccggt
      541 tcgcacagaa gctgagccgg gccgcccaga tgcagcgtgg tataacggct catggggacg
      601 ttttccatca gctgtttttt ggagaaaacc ggcagcagcc gttcgtacag cgtttgcatg
      661 gctcccgctt ccatggcgcg tctcctttcg ttccccaaac ccgggggtca aattctgatt
      721 ctgattgtat cgcagtttgg gcaggatgtc aaaacgaaac cggaaaaagt gtacgccaaa
      781 acgcgggcgg tcatgcgctg cgggggaaaa cgggcttcgt ttcccgctga ttgggcggtt
      841 tgtcaagcca aaaaaatcat gatatactgg gcaggctgac cggacttcgg tttctcgaag
      901 caccctgtgc aacgaagaaa acatgagatg ggcacgagcg ccaacggcgg cgtatatcga
      961 atgtttcacg ccggcagtga acgaagtttc cagaagtgaa cgaacggctt ttccatgacg
     1021 gtaaaattcg aatgcatagc aaacaaagga aaggaagaat ccccatgaaa cagcttacgt
     1081 ctcggcaaac caacgccgct gctttggcga tcgccgccgt ggcggcctgg cggctgccca
     1141 atgggtacgg gctgacggag aacgacagcc tgtttgcggg cagcgcgatc acgcttatcc
     1201 tgctggcggg gctgtacctg ctgctgaaaa acgcctttca gatcacgaat cggcggctga
     1261 atcggacggc ctattttctg ggcgggatgt tcgccgtagt gacggtggtc tgcgccgacg
     1321 tgcgggccaa caacggcttt tcggcgttcc gatggctgcc gttcctgtac ggggcgctgg
     1381 cggcggcgag cttcacggca gtgtacggcg cggcgctggc gctgctgttt cagggcatgg
     1441 atcgcctttc cgcgtccccg gcgaaagcgg agtcagtttt cagtaaggta ttgggcaact
     1501 gggcttttgc ctttgccctg ctgctggtgt gctggctgcc ggtatggctg gccttctggc
     1561 cgggcatttt cgcggccgac tccgcgacac agttttatca gtattataac gaggatttca
     1621 gcgcccatca cccgctgctg catacgctgc tgctgggctg gtgcgtcatg accggcgtgg
     1681 atctggaccc agagggctac agcacgctgg gtgtggcgat ctattccgtg gtgcagatgg
     1741 tgctgctggc cgccatgatg gcatgggcgc tgcgctggct gcgcaagcac cgcgcgcccc
     1801 tttggagccg cgtttgcgtt acgctgctgt tcgccctgtt cccgctttac tccattttct
     1861 cgttcagctg ccagaaggac attctgttca gcggattggg actgttgttt ctgttggaat
     1921 tggtggacgt gtggcaggaa ggcttcaagg cgctgcgctc cccatggcgc gtgattcggc
     1981 tgacggtcat taccgtgctg atgctgctga tgcgcaataa cggcgtgtac gccctggccc
     2041 tgctcttgcc ctttgccgtg gcgtgggcca agggggcgcg tgtgcggctg ggcgcgctgc
     2101 tgctggcctg tatggcgatg tacttcggcg tgaataccgg cctgaaagcc gcgttggagg
     2161 cggagaacat cgaccgggtg gagatgctgt ccatcccgct gcagcagatg gctcgcactt
     2221 tgcagcagaa ccccgacgcg ctgcccgacg acgaagcgcg ggagctgatc gagaccctgt
     2281 acccagacgg ctttgcggag tattattccc cggtgctggc cgacccggtc aagtgggcca
     2341 gcgattacga tgtggtggag gaacggatgg gcgatatcct gtccctgtgg gcgcggatgc
     2401 tgccggtgaa tctcaagccc tatatggaag cgttcctgat ccagaacctg ccctatctgc
     2461 tgcccggctc cgagatgatc tactatctgg acaccgaatc ccgggtcagc gtaatggagg
     2521 agaccttccc gctggtggag caaagccgca ttcccgcgct gaaggagctg taccgccagt
     2581 acgacaagac ccttaccctg ctgggactgc cgggcgtgcg gctgttgagc gacgcggcct
     2641 tccagatctg gctggccatg gcggggctgg cgctggccgt ttaccgccgc cagaagggct
     2701 acatggccgc gtttaccttt atcctgaccg tgtggttcac ctgcctgatc gggccggtgg
     2761 ccattatgcg ttacctgctg atcgccttct acggtgtgcc ggtgctgctg gccgggatgc
     2821 tggctgcccc ggaatgttcc acttccctct cccccaaaaa gtcggattga aaatgacaac
     2881 cgtatccgta accctctttg gatagggtcg ctctgctgct gccgggcggc cctgnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnaatcag ccttcgggta ttccatctcc cgaaacggcc
     3061 tgtgcaacga ggtgaaaaca tggcgggtat aacctacaaa tgccccaatt gcggcgctta
     3121 cctgaccttc gacccggaca gtcagcaatg gaaatgcccc ttctgttcct ccgcctttcc
     3181 cgaatcggag ctgaagggca aagcggaagc tttccaaaaa gaagccgaaa aagaagccga
     3241 aaaagaagcg aaagagccgt cctccggcgc gcaggtggtt tatcactgcc ccagctgcgg
     3301 cagcgagatc atgaccgatg aaaccaccgt ggctacccac tgctactact gccacagccc
     3361 cgtggtgctg cagggcaagc tgacggccga catgaagccg gacgaggtac tgccctttac
     3421 catcggcagg gagaaggcca tcgaggaaat ctccgaatgg atccgcaccc ggaaattcgt
     3481 tcccaaaaag ttcttttcca aggcgcagat tcaggaaatg agcggcgtct actacccgca
     3541 cttcgtcagc gaatgtca
//
LOCUS       DAWAEA010000122         3536 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4072_length_3536_cov_2.277219, whole genome shotgun sequence.
ACCESSION   DAWAEA010000122 DAWAEA010000000
VERSION     DAWAEA010000122.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4072_length_3536_cov_2.277219"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    437..446
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          698 a         1125 c         1058 g          645 t
ORIGIN      
        1 ggctggactt ggccggaatc gcctgttcca gcggttccgc ctgcaccagc ggcagtttgg
       61 agcccagtca cgtgctgtct gccatggggc tttcgcccga tatggcccgc agcagcctgc
      121 gcttttccct gggccgggac aataccgccg aggagatcga ccgggtgctg gccgtcctgc
      181 cccccattgt cgaagacctg cgggcgcgcc tgcgctcctg accctgtccc ttccgtttgt
      241 taaagaaagg aatctctatg ccggatattc atggaaacat tgagggcatc cgcaaaagcg
      301 ttctggacga aatgaagacc ctgtacgacc tccagctgga gccggatgtt tttctcccac
      361 aggaagtgct gtccgccctg tgcggctggt cagcctcctt caaccgggaa gtggccctgt
      421 acgtcacccg ctacggnnnn nnnnnnccag cagcgcttga gcatgatccg ctgcatccac
      481 acccacccca gcgccacggg tcagctcagc gacgttgacc tgagcgccct tctgtccatg
      541 cgctttgacg ccatgtgcgc cgtgggcgtg aacgcggagg gccagcccac cagcacccag
      601 tgcgcgtttc tggatcagca tgcctccgcc gggtatgtgc tgacggagct gctgcccgcc
      661 cgccgtctgc cccagcagga ctggctggat catatccagc aggccgacgc ggcctatctg
      721 gccgaggagg aaaaactgca aaaagatcat gagcgggcgg tcctggtagg catcgaaagc
      781 gaggaatcgc tggacgagct ggaggaactg gccaagaccg ccggagccga aaccgtgctg
      841 cgggtgctgc agaagcgccc ctcccccgat accgccttct gcatcggcaa gggcaaggcc
      901 gaggagcttt cccttcgctg tcaggccgtt caggccgatc tggtcatctt tgacgaggtc
      961 ctctccgggg tcatgcagaa aaatctggaa gaagtgcttc acatcaaagt ggtggatcgc
     1021 accgcgctga tcctggatat cttcgccgcc cgggcttcca cccgggaggg caagctgcag
     1081 gtggaaatgg cccagctgaa ataccgcagc cagcgtttgc tggggcaggg gctggtgctg
     1141 agccgtctgg ccggcggcat cggcacccgg ggccccggcg aaagcaagct ggaggtcaac
     1201 cgccgccgca tccgggagcg gctcactgac ctgcgccggg agctggacga aatggagaag
     1261 cagcgcaccc tccgccgcca gagtcgggaa aagggaggcg tgccggtggt ggcgctggtg
     1321 ggctatacca acgcgggcaa gtctactctt ttcaaccgtc tcaccggcgc ggacgtatat
     1381 gtgcaggatc agctgttcgc cacgctggac agcgtcagcc gccccgtgaa ccttccccac
     1441 ggcaccaaag ccctgctggt ggataccgtt ggctttatcc gcaagctgcc ccacgaagtg
     1501 gtggaggcct tccatgccac gctggaagaa gcccggctgg ccgacgtgct ggtgctggtg
     1561 tccgacgggg ccgaccccca gctgctttcc cgccgtcata ccgtcggtga agtactggac
     1621 agtctcggtg ccgtagacgc gccccgcatc gacgccctga acaaatgcga cctgctctct
     1681 ccctccgccg acgtgctgcc cggcgcgctg cccatcagcg ccgctacggg ccaggggctg
     1741 gacgagctgc tgaccgccat cgaggacgcg ctggacgcgg gcacggagga agtctccctg
     1801 ctggttccct tcgcccgtta tgccctgacg gacaagctgc gccgccttgg cagcgtcttt
     1861 cgcaggaaca caccgccgag ggcgtggtgc tgcgctggcg ggccgaaaag gcaaatgtgg
     1921 attacgccct ccgggaaggg gctgtactcc ttgaaacaga aagaaaatag ttgctatatt
     1981 attacaaaaa tgatacaata attgtagtgt gtccctgttt cccggcggct ctttcccggg
     2041 gctgccgaag aaagcacaga caaaattctc gtttaccagt tggaaagggt gtgaggaatc
     2101 gtggtgctgg gaaaaatgct ttccatcgtc ctaaccggcg cagcgctgct ttctggaagc
     2161 aaaaatgccg ccctgcctga aaaagatatc gacggcacca tttttctggt caatcgccag
     2221 cacgtcatca gtgagcagta cgtccctgaa cccctgcgca aaacgcaggt ctacggcatg
     2281 tcccagagta tgcgggaaga cgcggccacg gcgctggagg agctgtttca ggcggctcag
     2341 tccgagggcg tcaagctgtc cagcgtgtcc ggctaccgca gctattccaa acagtctctg
     2401 ctttatgacc gcaagaccaa acgcaccggc agcgaaacgg aggccgacaa gcttgtggcc
     2461 cgtccggggg ccagcgaaca ccagctgggg ttggccatgg atctggccac ccgcaacagt
     2521 tcgaccctgt ccgaaaaatt cggctccact cccgaaggcc aatgggtgta cgccaactgc
     2581 cagcggttcg ggttcatcgt ccgctatctg aagggctacg aggacgtgac cggctacagc
     2641 ttcgagccgt ggcacatccg ctatgtgggc ccggagcacg ccgaggccat cgccgcctcc
     2701 ggccttccct ttgaaacgta tctttccggc tggaagctgg aactttatga ttatctgctt
     2761 catcatgcta tgaactgagg tgctcccatg aggaatttcc gccgcgtttt gctggtctgt
     2821 ctcctgtgcc tgatgctgct ccccgtttcc gccctctgtg aagacggcgt agccatgggc
     2881 ggctcggagg tgaaagagcc ggaggtccgc tggacctacg gcgtttcgct gacggccctg
     2941 cagtccccct atctcattct ggtcaacgag cagaacccgc tggataagac cttcgagccc
     3001 tcccctctgg tgaagatgac cagcgtcaag cgggccacct ccgccaccat ctacctgcag
     3061 gaggcctgcg ccaaggcgat cgaggaaatg ttcgccgccg ctctggaggt gcaggaatac
     3121 acctacacca ccgtcaattc caagggcgag gaaacagagc acaccgccag ctatcccaac
     3181 ggcatggtgc tcatgctcaa gagcggctac cgcaagtacg gcacgcaggc caccatctat
     3241 cagaactatc tggcccgcaa caacggcgtg gacgacggct atgtcaacaa gcccggcgcc
     3301 agcgagcacc agagcggcct gtgctgcgac atcctcaacc gggactacgc cacccgggaa
     3361 tatatgacgc aggatttcaa atacaccccc gaagcccagt ggatgaagga aaactgcgcc
     3421 cagttcggcc tgatcctccg ctacactgag gacggcgaag cagccaccgg catcaaattc
     3481 gagccttggc acttccgcta tgtggggcgg gaagccgccg gttacatcag ctccac
//
LOCUS       DAWAEA010000123         3519 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4089_length_3519_cov_2.295901, whole genome shotgun sequence.
ACCESSION   DAWAEA010000123 DAWAEA010000000
VERSION     DAWAEA010000123.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4089_length_3519_cov_2.295901"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3199..3208
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          770 a          919 c         1097 g          723 t
ORIGIN      
        1 gcctactcgt taagcaaaaa aatagattta ggcgagctaa aatgcctgca aaaaaggaaa
       61 aatgcatatg cggaaatgct gtcaggctcc cgtagggcaa tggagaaggt catttccaat
      121 tatcagacaa aagtctgcta ttactttttg aaattgatgt cagtcaaaaa agcaaagcag
      181 gacaagctgc tgatcttacg cttgcagata gatgacgaac ttttcctgaa caacgcttct
      241 tatggaatcc aatgccgtgg aatcttccgc ctgctctggc gcgccctgca aacgcaaaag
      301 gcggagtggg tctatttcgt ttttttcgtg ctgggaaagc cctatatgcg ttatctggcc
      361 ttgaaagaaa aaatgttatg gagggatgga agaaagtgaa gcataaaacg acgcttcatt
      421 ttctggcgga gcgcaaccga agatatattc ccggcctgat cttgctgacg ttgctggaag
      481 tggcccgcgc ctatctgggc gtggagttcg ccatggtcac caggcaggtg atcgactgcg
      541 cccagggcgg gtcggcggaa aagctgacac gggccgcgct gctgctggcg gcggtggtcg
      601 cggggatcat ggtgctgtac gcctcggcgc aatacctgcg cgggtggctg cgggccacgc
      661 tggatcgcga gtggaaaacg cgcctgtcgc acaagctgct gcacggcgat tatgccacgg
      721 tatcggccta tcacagcggg gaactggtca accgtctcac cagcgacgtg gaggccgtca
      781 acggcggcgt gatcgccctg atccccaatg tggccgccct gttgacccgg ttggtctccg
      841 ctgtgtggat gatggccacc atggagccga cctttaccct gctggcagtc gcctgcgggc
      901 tcgtcgttat cggcgcgacc ggctttttcc gccagcggct taagacattg cacaagcaga
      961 tgcaggaagc caacggaaag gccaacggct ttgtgcagga gatcatggaa aagctgctgg
     1021 cggtgcaggc catgagcgtg gaagcggaaa cggaagcccg cgagaagaaa attctggaca
     1081 gccgctggaa gctgcaaatg cggcagcagc ggttttcgat catgggcacc acgggcatgt
     1141 atctggcctg ttacctgctg gaaggcgcgg cgctggtctg gtgtgcgctg cggctgatgc
     1201 agagcgaaat ttccttcggt acgctgaccg ccgtggtgca gctgaccggc cagttggaag
     1261 ggccgattct gaacttctcc ggctatattc ctcaatatgt ggccatgctc gccagcgcgg
     1321 agcggctgat ggagctggaa gaaatttcgg gcgttcccgt tcaaaaacga gacgtgcaga
     1381 cgctctttgg ggccgatacc tgcattgagg cggaggggct gtgcttcgcc tatgacgaca
     1441 cgaccgtctt tgacagcgcg tctttccggc tgcccttgca gggttctgcc gccattacgg
     1501 gaaattccgg catcggcaaa agtacgctgc tcaagctgct gctggggatt tacaaaccaa
     1561 cctcaggacg gctgctgctc agaacagggt cggaagaaat cccgctgaac gcggaaaccc
     1621 gcggtctgtt cgcctatgtg ccgcagggaa atctgctttt cagcggcaca ctgcgggaaa
     1681 acctgctgct gaccgcgccc gacgcgacgg agcaggacat ggagacggcg gcgttcgtca
     1741 gctgcatgga cgaatatctg cctcagctgg agcatggctt cgatacggtg ctgggggaaa
     1801 gcggcgcggg cctgtccgag ggtcaggcgc agcgggtcag catcgcccgg gccgtgctca
     1861 gcggcgcgcc ggtactattg ctggatgaag cgacctccgc gctggacgcg gtgacggaac
     1921 agcgcgtgct ggaacggctg aacgcgctgc cgggccgcgt ctgcatcgcg atcactcacc
     1981 gcccggccgc gctggcgctg tcagatcgcc aaatggtgat cgcagacgga aagatcacgg
     2041 ttcagacatt acgaaaagag gaaaaagcat ggatcagata agtcaggtcg tactgtctct
     2101 gctgcgttcg gcgctgtggg gagagaaaat tagttgccct gccgatacgg actgggaagc
     2161 ggtaacgaag gagctgcgta tgcaggccgt tttgggcgtt gtcgccggga ccgacctgcc
     2221 ggaaagcatc ccggcggagg tgcgtacccg atgggaaggc ctggcaatgc atcagggtct
     2281 gcatttttat catcttctgc acgatcagga cgagcttctg accctgctga aggaaaacgg
     2341 gatccccacc gttatcctca agggcatggc cgcggcggtg tactatccgt cccctgaaat
     2401 gcgctctatg ggcgacgtgg attttctggt gcccagagga aaggacgagc agacttacga
     2461 actgctgctg gccaacggct acgagctgtt tgaggaaaag gacgcggtcg acaagcatat
     2521 ttccttccaa aagggcgagg cgcgctttga aatgcaccgc tacttcggcc gctttgaccg
     2581 ggtcgacgcg atgaagtaca tggacgacct tctgcaaaaa gaaatgcaga gcattgaatg
     2641 gcgcgcctgc tcgggaagca gctttccgat gctgtcgtcg ctggccaacg gccttgtgct
     2701 gctggaacac gccgccggtc atttccggga tggattgggg ctacgccaca ttctcgactg
     2761 gatgatgtac gtccattcct gcctgacgga tgcgttctgg aacgaagcgt ttcagtcggc
     2821 ggcgcaggcc atagggctgg ataacttcgc caaggtgctg accaaaatgt gtcagaccta
     2881 cctcggcctg gcgggcgacc tgcaatggtg ccgggatgcc gacgacgaaa gctgccgcca
     2941 gatcatggac tatgtgctgg aacagggcaa ctttggcttt aagcaaatgg atgtggaagg
     3001 caaagtgatc cgtctgttcg gcaggcataa cgggctcgtc gagtggtgcc gtcttttgca
     3061 gctttccggc atgaatcact ggggtgcggt tcagcgacat cccattctca agcccttcgc
     3121 ctggatctat ggtataggcc gctatctgca tctggcgctc ggccggaaaa acgccatcgg
     3181 gctcgtccgg gaagaaaann nnnnnnnnga ccagcgggaa aaaatgtaca cggagctggg
     3241 agtcaatcga agcaaaatcc cagtcgttct gcaggacgat cagtttgtag agcggaagca
     3301 gtgacgaatc ccccgtgtgg gacaaaagaa gaacggcacc ccggatgtca gcgtggtatg
     3361 tgcggctgac atccggggtg ttttgctata gaaccattct ttgccatcgc cccctttttc
     3421 tgttacaata aacagagaaa tcatcatgcg gtcagggaag atagaatgct gaaacagctc
     3481 aggtattttc aatcggtggt tcggctgaac agcttttcg
//
LOCUS       DAWAEA010000124         3483 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4132_length_3483_cov_1.993291, whole genome shotgun sequence.
ACCESSION   DAWAEA010000124 DAWAEA010000000
VERSION     DAWAEA010000124.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4132_length_3483_cov_1.993291"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1863..1872
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2497..2506
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          597 a         1062 c         1086 g          718 t
ORIGIN      
        1 tgggacaggt gtagatgggc ttaagggtgt cgggagaaag cccgccagcc ttcagcgccg
       61 cggcgatctt gtcgttgagc aggttcatgg cgttgggcag ctgctggctg gagccttcct
      121 tccgctctac cagactggcc cggatgccgc ccagcaccag tgcgtggcgg gcgttgagca
      181 gctcccgcag accggggcag cgttcacagg cttcgtccac ccgctgctcg tacaggcgca
      241 ggttgtcctc ccggcgcttc atatagtcct gctgcagctg caagatgatc tgatcccggt
      301 tcacagcttc ttcgcctcct caatatcggc ctgactcatg ccgctgtatt cgtctggatc
      361 gtaatcccgc tgactgtatt gctgctcgat gacctttttc acgggcgcgg gggtcttggc
      421 ccgctcaccc gcggcggctt ctgcaaagcg gcggtgttct tcttcggcct cggcgatggt
      481 ggtgatgtcc ttatcccgcc agccggaaag cagcttgtcc atatagagca tgggttcccg
      541 gccgtgacct cggcggaggt cgtcagcccc cggtcgttcc acgcttccag catcagcagc
      601 acattctcga tggtgtgctg ttcgccccgc tttttaccca cctcccgggc cgccagcagg
      661 atcacgtcgt ctgtggccag ggcacgcatg gcctgataca ggctgcgcat cccctcgtcc
      721 accacggaga cccggctgcc gaaggcggcc agcatggccc gcacccggga ggactcgtcc
      781 tcctcgccgc gcagctgctt ttccaccgcc gtggtggccg taccgccgct gcggtcgcgg
      841 atgccggaca aaatgccgtc cagatagcga aagctggggt tggtgcccgc cgtggtctca
      901 ctgcaggcca cctcgatggc cttgggcgca tacccccatt cctgtgtcca cttgacgtac
      961 aggtcgatct cgtccatggt gggggaacgg tggacgttga agcggctcag gatcttcttg
     1021 gcccccttcc acgcggcttc ggagcggctg aataccgctt cggcggcttc gggggtgcgg
     1081 gcattctgtt cacacagctc gttgaccacc ttctgcgcgt ctttgaaagg gaacttgatg
     1141 ccccgggtgg agatcatgtg gcgaatcagc atgatgacca cttccggggg cagcttctgc
     1201 tgctccaccc actcgtaggc cagcacggtc tcgctgccgt gcagctgccg ccgggagccg
     1261 aacaggtcgc agagcgcccg ggcaaattcc tcgtagggtc ggtcctgcgg cgcggattgc
     1321 ttcatcaaca acgcctgctg gacactggca aaccgataca ccggcggctg atcctgcacc
     1381 cggctgacca gacggcaacg ctcccagtat cggagcgcct gttccacgtc ggcccgatgc
     1441 atttccagcg ccttggccag atcgtccagc acgccctctt cttccagcat accgccgtga
     1501 gcatacatca ggccatacag atagaccttt acatagccgt ccggggcggc gggcataaat
     1561 tcgcacaaaa aggcgttgga gaccgccgta gcgtcccaga gcagggcgga ggtatcgcat
     1621 tggaaggtgg gcatgttgaa aaacctctct tagtgcaggt tgcagccgta cagaccgctt
     1681 tgttggaaag cggcggtcag gtagggcttg gtgcgcatca gcgcggcgta cagggcggaa
     1741 ccctgctcgg ataaagtgta ggggacgatc aggcggctgg aaagcgcctc gtctttttcc
     1801 ttttggaaca gatgcaggcc gcagccggtg agcaccggca ccagcagcgt acatacggag
     1861 cgnnnnnnnn nncagccggt ctgttggaca gcctcccgcc agacgcgcag ggcttcgtca
     1921 agctcctgct gggtcgcgtc ctcccgctga gtagcggcgt aggcagtctg cagcagggaa
     1981 gcaacggact cctgcgaggt ctgcttcacg ggctgaccgt tgacggtctc tgtttcgcta
     2041 ttggaagaag caccgctttc ctgctgattg gccagtgcgc tgacgtcaaa ggcgatctgt
     2101 gcgccggaca gagcggggga caaatcgatc agttcactgc cgttttccag ccacgtctga
     2161 acgcgcatca ggggcgcgcc gtcgccctgc agggtataag cggcctgttc ctgtgcggaa
     2221 agctcgctgt tggagaacac gggaatcagg tcaaaacgga aaacggtgtt gcccttgggc
     2281 aatccgcttt gtctgcggta ctgatccacg ccgtcgctca ccagatcccg cagggagaag
     2341 gtcagcgtaa cgccgtttaa ggagaaggcc acgtgttcca catgcttgtt cagcagctgc
     2401 tgaacgtgcc agtagcccag ccgcagggtg cggccctgcc agaggggcgt gccgttccca
     2461 tcggtttgat agccgccgtc tttgctcagc tcggcannnn nnnnnnaaag ggccgctcct
     2521 cgccgctttc ggtgtcccgg ataatggaac cgaagtaagt tttatccaga ttcagcatat
     2581 cggcaagccg gtactgtacc ttgagggacg tgccgtcgct gaagtggatt tcttcgtaga
     2641 ccgggtcgat cacgctcaac gggtcgaagg gcagatagtg gtgatagttc cagtcgatga
     2701 aaaaatggcc gctgtccggg gacagcacga aacggtaatg gttgaccgtc tggccgtttt
     2761 catccacggc ggacagtttg tcggtggaat aggcgtagtt gccgtatccc tcgcccttgg
     2821 cgcgggtcag ccggccccgc agtcggtccc ggccgcaaag ttcgtcggtg tcaaactcaa
     2881 attccgggtc gtcttcgccc ttctgcttgg tcgcgtccag cgcgtagacg tagatcaggc
     2941 ggcgcttgat ctccagcggc ataacgactt ttccgggcaa atattctgga tcgtcttcca
     3001 gaacggtaat ctcggcgtaa tagctgcctt ccttcgtggg cggggtctgc aaaggcgtgc
     3061 cgtcctccag gaagtaagcc actttgcatt tccggggcag ccgggagatc tggaagggct
     3121 gaccgttgta ggtcttgctc tgcccggccg gttcgtaagc cgcccgggac tcgatggtga
     3181 tggactggtt tttgtagaag gtgaaagcgt ccgccgttgc ggcgctgagc cgcgcgtcct
     3241 tttcccgggc ggcaaagacc cgcagactga ccacgtcgcc cagctccagc ccgggcaggt
     3301 acagcgtggt ttcgccgtcg gcggcataca gggtctgtgc ggaggcgttc cacgaatccg
     3361 tcagggaaac gggcgcgtcg cctaccacat actgtacgcc ccaaacgccc gcgtttttca
     3421 gcgtgacgga caccacgccc cgattggtca gggacgcggc ggtgatttcg ggcgcttcgt
     3481 cga
//
LOCUS       DAWAEA010000125         3475 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4141_length_3475_cov_2.722515, whole genome shotgun sequence.
ACCESSION   DAWAEA010000125 DAWAEA010000000
VERSION     DAWAEA010000125.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4141_length_3475_cov_2.722515"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2212..2221
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          758 a          971 c         1105 g          631 t
ORIGIN      
        1 tcttctgggt ggcccgcatg atcttctccg gcatggagca gatgaagaag gagcccttca
       61 agaccgtgtt catccacggc cttgtccgcg atgacaaggg ccgcaagatg tccaagtccc
      121 tgggcaacgg aattgacccc cttgagatgg ccgagaagta cggcgcagac gctctgcgct
      181 tcaacctgat caccggtaac agccccggca atgatatgcg cttcttcgtg gagaagtgcg
      241 aagccatgcg taactttgcc aataaaatct ggaatgccag ccgcttcgtc atgatgaact
      301 tgtccatcga caaggtcgag ctgcccgaga agctggagct ggaggacaaa tggatcctca
      361 gcaagctgaa taccttgatc cgcgaggtca ccgacaatct ggaggcctac gagctgggcg
      421 tggcatccgc caagatctac gacttcatct gggacaccta ctgcgactgg tatatcgagc
      481 tgaccaagac ccgcctgaat ggcgaggatg cgcaggcaaa gcaggccgcc gagaatgtgc
      541 tgtgctatgt gttgctgcgt atcctggaac tgctgcaccc gttcatgccc ttcatcaccg
      601 aggagatctg gcaggcgctg ccccacgagg gccggtacct catctccgcc gagtggccgg
      661 agtatcagga tgcgctgcac ttcccggagg ccgaggccgc tatggaggcc gtcaaggacg
      721 ccatctccgc catccgtgcc cgccgcgccg agatgaacgt gccgcccagc cgcaaggctc
      781 aggtcatcct tgtgacggcg cagccggaga actaccagtt gggcgcacac ttcatcaccc
      841 gcatggccta cgccagcgag ctgacggtgc tgaccgccgc gcctgccgac ctgaccggca
      901 tggtgaccgt cgccactcac gacgccacgg tgtatatgcc catggcggag ctggtggaca
      961 tcgccaagga gctggagcgc atcgccaccg agcgcaaaaa ggcggaggag aacctccggc
     1021 gcatcgaggc caagctggcc aacgagtcct tcacctctcg tgccccggag aacgtggtca
     1081 acgcagagcg cgaaaaggcc gaaaaggccc gggcgcttat tgctaagctg gacgcctccg
     1141 ctgcggcgat gaagctgtaa agccatagtg cttcacagtt attctgatgt aaaaacggaa
     1201 gcaaagccca gcccgccgtg tgcaggaccg gacaaacaaa aaagacgcct ttcggcgtct
     1261 tttttgtttg ttcgcaaacg gaaatatgcg ctaaatacac ggctcgctcg ctgtgctgga
     1321 tcgaccgccg ttccggcacc gaaaaacatg gtgctttcca aaatttttcg cccttctgac
     1381 ggatttcgac aaattccgcg cgtcggatag tctataatca acgcagaaca tcgaaccgcg
     1441 gagttttttc tggcaggcac ggcctgcggc acacaccaaa gaaaaaggag cgttcaaatg
     1501 gaagtatcgg caaaacacga gggccaaacg ttgacattct ttctggtcgg cgagttggac
     1561 caccatgggg caaaaggact gctggaaaat ctggagcgcg agatcgagcg gacgctcccg
     1621 ctgtcgctgg tgctggattt ttccggcgtc acatttatgg acagctccgg catcgccgtg
     1681 gcgctgcggg gttggcagcg gatgcgggag atgggcggcg ccgtaacgct gtatcatgtg
     1741 gcgcagcagc caagaaaggt gttcgaagcg tcgggcgtcg gacgcatggt gacgatcgtt
     1801 tgaggaggga gtacataata tgacacgagc tgaaaactac gtgacactgg aattcctgag
     1861 ccgcagcagc aacgaggggt ttgcgcgggt ggcggcggca ggctttgccg cccaactgga
     1921 ccccacactg gatgagctgg gggacatcaa gaccgccgtc agcgaggcgg tgaccaacgc
     1981 catcgtccac gcctatccgg atcagttggg taaggtggtg atgaagctga agatattgaa
     2041 aggggggatg ctggagatca cgatccgcga ctgggggcgc ggtatcgaaa atgtggagca
     2101 ggcccgccgt cccatgtata ccaccggcgg cagcgagcgc agcggcatgg gctttacgat
     2161 catggagagc ttcatggatc ggttggtagt gcggtctgtt ccgggaaagg gnnnnnnnnn
     2221 ntcgcgccgc ggctggcggt acgatgagcg accggctggc actgctggaa cgggcccagc
     2281 agggcgatga ccgggcggag gcgcagctgc tgacggagaa cgcgggattg atctggtcca
     2341 tcgtccgtcg ttacaatggc tgcggtgtgg agacggatga cctgtatcag ttggggtgca
     2401 tcggctttgt aaaggctgta aaggggtttg acctcacata cgggacgcag ttttccacct
     2461 atgccgtgcc gaaaatcgcc ggcgagatac gtcggttcct ccgggatgac ggcccggtaa
     2521 aagtgggccg caccatgcgg gaaaaggggc agacactttg ggcggcacgg gaacggctgc
     2581 aagggcgtct gggctgtgag ccaaagctgt cacagctggc agcggagacc ggcatgacac
     2641 cggaggagat cgccgccgtg gatctggcgc tgatcgcacc ggactctctg cagcaggaga
     2701 ccgcggaggg cctgacgctg gagtccacac tatgtgccga ggagcccagt gtctctctgg
     2761 tggagcgtat cgccctgcgg gacgccatcg acgcactgcc ggagaaagaa cggaagacca
     2821 ttctcctgcg ttttttcaag gggttgacgc aggagcagac cgcccgcatc ttacaggtat
     2881 cgcaggtcca ggtctcgcgg ctggagcgcc gcagcctgca aaagctgcgg gacctgctgg
     2941 cagaggagtg acttcgggca gagacggcgt aaaaaggggg gcgtctcgac agcagcgcga
     3001 ttccgaatgt aaaatggatc aggacagagg gggctctatt gcggaacgct catcccgtta
     3061 tcggcgggat gggcgttccc tcgtaacgga aaggtgaaac gcgtttccgg cagatgtctc
     3121 ttagccgtcc gttccggagg caccaggcag attcgccaag gcaccctgcc ctttttcgta
     3181 gtccaaaggc aaaaactccg tcatggtccc ccgataccgt ttcggcataa acgtgttctg
     3241 gcaccgggga ttttcgcgct cccgaatggc gacgctttgg aaaaactgtt tccacagcga
     3301 gcgaaagtac aactcgtcct ctccgggcag cgcgggctga aagtgctcca tccgcataat
     3361 gcgggaacgg cctccggcat acagcagcaa ctctcgatgg gtgcggtcgt agataaaaaa
     3421 ctgctcgttg gcgtaacgct ggcaaaaatg gccgcgcagc agcggcagca cacgg
//
LOCUS       DAWAEA010000126         3470 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4149_length_3470_cov_2.549634, whole genome shotgun sequence.
ACCESSION   DAWAEA010000126 DAWAEA010000000
VERSION     DAWAEA010000126.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4149_length_3470_cov_2.549634"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          612 a         1081 c         1054 g          723 t
ORIGIN      
        1 gggtctggta ggcttccctg caggggaaaa tgattgtgaa aatagctcgc cgttcaggcg
       61 ggctattttc gttgtagggg ttgagcccca gcgcctcggc ttgtgcggca gaccttcccg
      121 gcagcatggc atgaagcggg aaagttgccc gtatcgctat aacgcgagtc gattttccac
      181 tcaaaaaatg tgaaaaaccc gctttcttct gtctgttgaa gaaagcgggc tttttatgcg
      241 tttgagaggg gtcaatgctt ggaaagcagg gcggtgacgg tcataaatcc ggcggcggcc
      301 ccttctacca gaatgacctg accgttcagg gtaaagaggg atagcagcag cttggaatga
      361 gccagaccgg cggtttccag atactgatag cacagagtca cggccagcag ggccgcgccc
      421 gcgtagagcc acggctgcaa gcccttggag tggcagcgca aacctgccag cggaatgatc
      481 agcgccagca taacgcccag cagcagaccc ttgagcagga aagaccagaa cagccctgcg
      541 gccagaatgc cgtccagcca gcggtacaaa aggcacagca ccagacagag cagcagcggg
      601 caaaacgccg ctaccagctt acggagcaac atggctctcc tcctcccttt ggagaaaaag
      661 cagggtctcc tgccgttctt tgtcggtcat gggggccgtt tcgaacaggt cggggcgcat
      721 ccgggccgtt accagcaggg cctgctgccg ccgccatgcg cgaatcttgg catggtcgcc
      781 gttcagcagc acttcggggg cttccacgcc ctcaaattcc cggggacggg tatactgggg
      841 atattccagc agaccgccgg tgaagctttc ctcgtcggtg ctgtcgctgc tgcccagcac
      901 gccgggcaca tatcgggcca ccgcgtcgca cacggccatg gcggccagct cgccgccggt
      961 caggacgaaa tcccccagcg acagctcctc gtccaccact gcgtcgatgg cccgctgatc
     1021 cacgccctcg taatggccgc agagcaggat gaagttctct tcctgcgcca gctccaccac
     1081 cttttgctgg gtgaaggtct tgccccgggg gctcatatag atacatttgc ccgtataccc
     1141 ctgcgcgcgc agatggcgca ccgcgtcgca gatgggctgg gccagcatca ccatgcccgc
     1201 gccgccgccg aaggggtaat catcggtgtt tttatgcttg cggtcgctga aggggcggat
     1261 gtccaccggc tcgatctcca ccagcttttc cctgcgggcc cggcccagaa tgctggaatg
     1321 gagcacgctt tcaaacattt cgggaaagat ggtcagaatg gcgatcctca tataccgcta
     1381 cctcgttcag ggtttcttcg ttgagtacga tgcggccagc ctccacatcc agcgagacca
     1441 ccaccttctc aatgatgggc accatgacct cgcctgccgg agtgtcgaaa acaaaaatgt
     1501 cagtgccgcc gggggtcagc acatccttca gaacgccgat ctcgccgccc ttggtatcca
     1561 cggcttccgc gcccagcagg tcggcgatga agaactggtt ctccgccagc tccgccgcgt
     1621 gggcccggtc tacgtagagc atggcccccc gcagggcttc ggcggcgttg cggtcgttga
     1681 agccctccag cttcaggtac acgtcgtcgc cctgcacccg gcaggcctcg atggccagcg
     1741 gctcaaagcc ctctccggtt tggcgcagca ccgcgtccag ctcctcgaag cggtaagggt
     1801 cgtcggtgaa atggcggagc ttgacctcgc cccggatgcc ctggggccgg acgatctcgc
     1861 ccaccagcag atgcgtttgc ttcataaaaa cgttctcctt agaaagcgcg caccggcagc
     1921 ctttcgggag accggtgcgc gatgggttat tgaatttcca caaagacggg cttggcgtcc
     1981 ttggcggtag cggcgcgaac cacagaccga atggccttgg cgatgcgtcc ctgcttgccg
     2041 atgaccttgc ccatatcctc gggcgctacg tgaagctcca aaatcgtcgc ttccggcccg
     2101 acggtctcct caacggtcac ggcgtccttg ttgtccacca gcgaccgcgc gacgtattcg
     2161 acgagctctt tcatgaggct tagccctcga tgacgccgct cttcttgagc agaacgcgga
     2221 cggtgtcggt gggctgagcg ccggacttca gccacttctg ggccttctca gcgtcgatct
     2281 tgatggtggc ggcgggctcg gtcatgggat catagtagcc gatctcctcg atgaagcggc
     2341 cgtcgcgggg gctgcgctcg tcagccacga ccacacggta gaaaggcttt ttcttcatgc
     2401 ccattctctt caaacgcatc ttaaccataa ttctgttcct cctagtattc tgattggtga
     2461 gggatttgta tctgaatcgc cagcgggatg cccgggcggt gttcagcgga ccgggggatc
     2521 aaaaggaaaa gcgccgtttt cccctgcgca tcagcttgtt gcccatcatt ttcttcatca
     2581 tgtccttggc ctgctcgaac tgacggatga gggcgttcac gtcctgcacg gtggtgccgc
     2641 tgcccgccgc gatgcgcttg cggcggctgg cgttgaggat gccggggttc cggcgctccc
     2701 ggggcgtcat gctgcggatg atggcttccg gcttcttcag ggcgttttcg tcgatctcca
     2761 cgtcctgaag cttgttggcg ccgggcagca tgctcagcat ggactgcagg ccgcccattt
     2821 ttttcatctg ctgcatctgg ctgaggaaat cctccagcgt cagctcctgc gggccgcgct
     2881 tgaccagctt ctcggcttcc ttttcatcga aggcctcctg cgccttttcg atgagggtca
     2941 tcatgtcgcc catgcccaga atacggctgg ccatgcggtc ggggtagaag ggctcaatgt
     3001 cggagagctt ttcgcccgtg ccggagaact tgatgggctt gccggtgacg gccttgatgg
     3061 acagggctgc gccgccgcgg gtgtcgccgt ccagcttggt cagaatcacg ccgtcgatgc
     3121 ccaggtcgct gtcgaaggtc ttggccacgt tgacggcatc ctgaccggtc atggcgtcca
     3181 ccacaaacag gatttcctgg ggcttgatct ctgccttgat gcggcgaagc tcctccatca
     3241 gcgcggtatc gatctgcagc cggccggcgg tgtcgatgat cagcacgtcc cggccgtagt
     3301 ggcggcactg ctcgatggcc tcctgcgccg tcttgaccgg gtcctgcgtg ccgtgctcgt
     3361 acacgggcac gttcacctga gagccgacca cctgcagctg cttgatggcg gcggggcggt
     3421 aaatgtcgca cgcggccagc agcggctttt tgccctgctt ctgcaggtag
//
LOCUS       DAWAEA010000127         3466 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4152_length_3466_cov_1.499853, whole genome shotgun sequence.
ACCESSION   DAWAEA010000127 DAWAEA010000000
VERSION     DAWAEA010000127.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4152_length_3466_cov_1.499853"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    815..856
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1543..1742
                     /estimated_length=200
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2056..2065
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          560 a         1009 c          982 g          663 t
ORIGIN      
        1 cccatgggca gtgcggccac ctgtacgcag ctgcgggcgg gataaacgcc atcgaagaac
       61 tccgcataca ccgtattgac ggttttgaag tccgccaagt ccttaacgaa aatggtggtc
      121 ttgaccacat cgccgtagga agcgcctgcg gccttcagga tctcgcccac attgcgcatg
      181 gaggcgcgag cctgcgcttc cacgccctcg gccagcttgc cggtctccat gtcgatgccc
      241 agctgaccgg aggtatagac catatcgccc accttccagc cctgcgcata ggggccgatg
      301 gcggcgggag ctttttccgt gtgaagtacc tgtttcatga ttcatttctc ctttttagtt
      361 tcattcatcg gggaacctgc ttccagcaga cggcggttca gttcccgcag ggggtcctcc
      421 ggcagcttga cgacccgacg gtcgtaggtc atgatgccat tcagctcgtc ctccacgtct
      481 gtcagctgcg tataaacggc ggcgcacagt ccccggggga tggcgggcag aatctgctcc
      541 tccatcagct tgcggtaagc ctgccacagc gcttcccggt cggaaaagcc cttgtagcca
      601 tagttcacgt cgttccagca gtggccctcc agccgcaggt tgtagccgcc aaattcgctc
      661 aagatcaccg cccggcccag cttgtccggc cggaaacgat aaggccggaa atacacgtgc
      721 aggctttgaa ggtcgccgat cttctggtcg tgccagccgc tggcgtggtc gatggggcgg
      781 ctttcgtcca ctgaacggat gatgcggcag gcgtnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnattt ttgactgcaa tgaccgccag gatggacacg atgcggcagg
      901 cgtccgccgc gtcaaactgt ccccagcctt cgttgaaggg cacccacagc acgatggagg
      961 gcacgctttc cagctgcgtg atcatttccc acagctccgc gcggtattcc tcccgcccct
     1021 gccgactttc ccgggcaaat ttccgatagc ggttgtcccg atgatgaatg ccggtgacca
     1081 gtgggaagct gatggtggaa aagtgatact ttccgccgcc gttgggcata tcctgccaca
     1141 ccagcagtcc cagtcggtcg cagtgataat accatcgcat gggctccacc ttgatatgct
     1201 tgcgaagcat attgtagccc aactttttgg cagtctgcag gtcgaaaatc atcgcttcgt
     1261 cggcgggagc ggtgtataag ccgtcgggcc agtatccctg atccagcagc ccgttatgaa
     1321 aacaggggcg attgttcagg cgcaggcggc gaacgccatc cttcccggcg cggacttccg
     1381 ttttccgcat ggcgaaatag ctttcgaccc ggtcttcccc aaaatgaagc tccaacgggt
     1441 acagccatgg gtcgtcgggg ctccacaagc gctgatcccg caccggcaga cgcacgggtg
     1501 ttccgacagt aaacgcaaag cgctcgccct gcaacacggc ggnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nncccgggcg gctggcgaac acggtcaatt ccaattctcc gcggtcgata tccgggatca
     1801 gacgcacccg ctggatgtag gcttccggca cccattccat ccagaccgtc tgccaaatac
     1861 cgctttgagg ggtgtaccag atgccgcccc gcttcgtgcg ctgcttgccg cgggtgtgcc
     1921 aggacgcgtc cgtatcgtca tagacccgga cgatcagttc attctttggc tgaataaagg
     1981 ccgttgcgtc cacagtaaag gcattgtagc cgccccggtg ctcccaggct ttttggccgt
     2041 tgacaaacac cgccgnnnnn nnnnncctga tccaccgcgc cgaaatgcag cagcagccgt
     2101 ttgcccgcgt tccagttttc cggcaaagca aaatatccgc ggtaccagag cgtctctgcg
     2161 ggccgaaggg tgcggcccac gccgctcagt tcgctttccg gcgaaaaggg caccagaatg
     2221 gagccgtccc aactttccgg aaagggttct tcggggaggg tgggacgaat ggcgtattcc
     2281 catgggccgt tcaggcactg ccagccttcc cgccgaagct gggggcgagg atattcggaa
     2341 agaggcattt cccggtccaa tttttctcca aaaggggtca gcatcgtctc cttctccttt
     2401 ttgcagcaaa attcaggctt tttgagtata ccctatcccc gccaaagttt caaggcggtg
     2461 ccggggcttc ggggcacggt cgaataggct ttcctgagcg ggaggcgagc aaaatgaagc
     2521 aaagcgcggc gaaacaaacc ctgaagatca cggcggtcaa cggcattgtc cgtgccctcg
     2581 gaatgctgct gcgtattctg ctgtcccggc tgctgggggc ggagatcatg ggcattgcgg
     2641 agctgagcca gagcgtccat atgctggcca ttaccccgct gacctccggg ctgccgctgg
     2701 cggtgagccg catgacggcc aagacccgga cggaggaccg cacaaagccc ctgctggccg
     2761 ggctttggct ggtgcgcata gcggcgctcg tgctcatgcc cctgatgctg cttttttcgc
     2821 cgcagctggc acggctgacc ggcgatgtgc gggtgcttcc atccctgtgg tgcagcgcgc
     2881 cctgcatcct gattctgggc tattcggcgg tatacaacgg ctacctgtac ggggtggaac
     2941 gcagcctgct gcccgcgtgg tcggagctgg tggagcagct gctccggctg acgatctgcg
     3001 tagggctttt gttggcgctg ccccatctgg cggccccgtg ggcggcggcg gtgccggtct
     3061 tttccaccat ggccgctgag gtgctggggc tggcctttgt actggcggta cttcgtctgc
     3121 cactgtcgcc catggagacc gcccgaagct ggcgaaagcc cgtgctgcgg ctggcgctgc
     3181 ccaccaccgg cacccggctt gtgcaaatgc tgctccgggc cgtggaggcg gtctggatcc
     3241 ccctgcggct gcaggcctcg ggccttgccg cccgggaggc cactgcccgg ctgggaatgc
     3301 tttccggcat ggtgacgccc gttctgatgc tgccctgcgt gttcaccggt tccctgtcca
     3361 tggtgctttt gccgaggatc gcgttggcgg agaaggaacc caaagaactt cggcggctgg
     3421 tcggcaagtg cttcctgacc gccgccccag ccgggctggg cagcac
//
LOCUS       DAWAEA010000128         3463 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4157_length_3463_cov_1.619718, whole genome shotgun sequence.
ACCESSION   DAWAEA010000128 DAWAEA010000000
VERSION     DAWAEA010000128.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4157_length_3463_cov_1.619718"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    413..540
                     /estimated_length=128
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    863..1001
                     /estimated_length=139
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          541 a         1021 c          942 g          692 t
ORIGIN      
        1 gacggcctac ccgcagatag tcgtccacag ggtgcacctg ctccagcccc agcgtagcgg
       61 ccagcgaatc ccccccgccg ccttccgcct tgtcccagtt ggtgtgggcg gcaattaggg
      121 acatgcggtt ttccacgatc tgggaaacgg cctgtccgat ggggtccgca tagtgtatgc
      181 gccgcagcgg gttgaatatg agcggatgat gggtcacgat cagctgtgca tggagacgaa
      241 ctgcctcccg taccagtgcg gaggtcgcgt ccagcccgaa gaccaccgtc tgcaccggca
      301 gccgttcgtc gccgatcaaa agccccacgt tgtcgaaatc ctcggcactg tcaaaggacg
      361 caaggtcgtt cagccagtcc agcgcgtccc gaacgttcat caggctcata agnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 ccgccgttcc agcaagtcgc gccacagcgg cggacgttcc cggagcagta ccggccccag
      601 cagcagctcc cgctcgtcgt agacgcgctc gccgtccaac gcgggggtgc attgcatcaa
      661 cagataatat cgccgatttt ccttgacagg ctgttcctga cggatgcggt agccgatccg
      721 gcacagggta tcccgcacaa ggggcaggtc ggtttgagcg cccagcacca gcgatgcgcc
      781 gtggagccgc ccgcagcctt cttccaaaat ccgggagagg gtctgcccgc ccatgcctag
      841 gacaaaaacg gtttccgccg ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nattctttgc tttgttcaga
     1021 gaaggcgcgc tgatgtccgc gaccagaacc tgctttgccc ggccgctttg caaaagcgcg
     1081 gcgctgagcc ggccgtggtc ggctccgatg tcggcgcata ccccgcaggg ctgcgccagc
     1141 agaagggccg cctgcagacg gctgtccaga tggatcatac tcatcttagt ccagataatc
     1201 ccgcagtttc ttgctgcggc tgggatggcg cagtttccgc agagccttgg cctcgatctg
     1261 acggatgcgc tcacgagtta ccttgaattc cttgcccact tcttccagcg tgcgctggtg
     1321 tccgtcgtcc agaccgaaac ggagccgtag caccttttcc tcacgggggg tcagggtgtc
     1381 caacacgtcc agcagctgct ccttcatcag ggtgaagctg gcggcctcgg cgggggcggg
     1441 agcgtcgtcg tcggggatga agtcgcccaa gtggctgtct tcttcctcac cgatcggcgt
     1501 ttccagagac acgggctcct gcgcgatctt gatgatctcc cgaaccttgc tctcggaaat
     1561 gttcatcacc ttggcgatct cttccggcgt aggctcccgg ccatattcct gcaaaagctg
     1621 gcgggagacc cggatcagct tgttgatggt ctccaccatg tgcacaggaa tgcggatggt
     1681 gcgggcctga tcggcgatgg cgcgggtgat ggcctgacgg atccaccacg tggcataggt
     1741 ggagaacttg tagcccttgc ggtagtcgaa cttgtccacc gccttgatga ggcccagatt
     1801 gccctcctga atcagatcga gaaacagcat tccgcggccc acgtagcgct tggcgatgga
     1861 aaccaccaga cgcaggttgg actcggtcag ctttttcttc gcttcctcgt cgccctcttc
     1921 catgcgcttg gcgatctcga tctcttcctc tgcggacagc aggggaacct ttccgatctc
     1981 cttcaggtac atgcggacag ggtcgtccac gctgatgcct tcgggcacgc tcaggtcgat
     2041 gttttccggc tcgatcagtt cttccggggg aacggggtca aattcgttca ccacgtccac
     2101 gcccaggctt tccagcactt catagacctt gttgatctgg tcggcgtcga tctccagctc
     2161 catgagctgg ttcgctactt cttccttggt aatggagccc ttggtcttgg cttcctcgta
     2221 cagttcgttg agctttactt tgaccgcttc ccgcgatgga ttttcactca accgtctcac
     2281 tccttctatg tacacagggg gaatcgttta cttcagcttt ttccattctt ctgccaatag
     2341 ataggcctga cgcagttcct cttgctgccg ggcttcttgg gtattggctt ggcttttggg
     2401 taattcaatg gtttcccgtt cccggcggag ctttttcagg cagtcctgcg ccatgtgcat
     2461 cagcccttcg tcgtccaact cagtgctgat gctgagaatg tcgccgatga ccgcccggcc
     2521 ttcgtcatcc ggctggcgct ccatcagcgc cgccgggctt tcgccgccca gcagggcctc
     2581 gtgcacggga ttgagcaaag tcgtccgggg aaacgctgcc ttccggtagt cggcctgtgg
     2641 aaagcaccgc cagcagcgtc cgggccgccc agtccacctg agagacctct ttggcctttt
     2701 ggcggaagct ctcccgcttg ggcacgtcca ccttttcagg ggcggcaggc cgaacgccga
     2761 tctgttccag cagcacttcc cggtcaaagc cggtctccat ggccagccgc cggacatagt
     2821 tttccagctc cacgggctcc ctgaccttgc ggaggaaccc tgcgcaggct ttggcgtact
     2881 cggtgcggcc ttcctcggcg gacatatcgt gcttctcttt ttcccgaagc atacggtagt
     2941 acacgccgga gatgggcttc agggcctcga aagcttccag cccttcctga cggatgaact
     3001 cgtccggatc cagcccgccg ggaaaatcca gcaccttcac cggaacgccc gtctcctcga
     3061 gaatatccag ccctcggaga atggcgtgct gcccggcgct gtcgccgtcg taggagagcc
     3121 agacttccgg agcgtagcgg gacaggagct tggcctgttc cggcgtcagc gccgtgccca
     3181 gcgtggcggc tacgccttcc acgccgaact ggctcaggga gaccacgtcc atgtagccct
     3241 ccaccaggat cacccgcttg agccctcgcg ccttgcgcag cagattggct gcgaaaacgt
     3301 tgtatcgctt attgaaggcg ggggtatcgg aggtgttgag gtacttgggc tgcacgtcgc
     3361 cgatggcgcg cccgccgaag ccgagcacgt tgccgtaggc gtcgatgatg gggaacatgg
     3421 cccggttgcg gaacatgtcg aagacccggc cgtccctttc cag
//
LOCUS       DAWAEA010000129         3445 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4192_length_3445_cov_2.159587, whole genome shotgun sequence.
ACCESSION   DAWAEA010000129 DAWAEA010000000
VERSION     DAWAEA010000129.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4192_length_3445_cov_2.159587"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          658 a         1075 c         1070 g          642 t
ORIGIN      
        1 tcgttttttc cgtcctctat tatagccgcg ccgccgccgc cgcgcaaggg cggaaaccgt
       61 ttgtgaatat atggttaaaa gtgtcccgct gtctattgac ggcgctggcg catcctgcta
      121 ctctttacct tgtaagaatg atcctctctg gcgaatcggt cattcggaaa ggaaaaacct
      181 gtgtgagcta catttccttt gaaaacgtta cgaaagaata ccagatgggc ggcgaaaagg
      241 tgctggcagc caacggcgtc agtttcgaga tggagcaggg cgaactggcc gtggtgctgg
      301 gccccagcgg tgcgggcaag accacgattt tgaacctgct gggcggcatg gacaaggtca
      361 cctccggcaa gatcgtcgtg ggcggcaagg agatcaccgg cctgtccggc cgggagctga
      421 cggaataccg gcggctggac gtgggcttcg tgtttcagtt ctacaacctg atgcccaacc
      481 tgaccgcgct ggaaaacgtg gagctggccc gtcaggtgtg ccccagagcg ctggatcctg
      541 tggaaatgat gcggcgggtc ggtctgggag agcggatgaa caacttcccc gcccagcttt
      601 ccggcggcga gcagcagcgc gtttccatcg cccgggccct gtgcaagaac ccggcgctgc
      661 ttttgtgcga cgagcccacc ggcgcgttgg actccaagac cggcgcgcag gtgctggctt
      721 tgctccggga cgtgagccag acctaccaca ccaccgtggc gatcatcacc cataacgccg
      781 gatcgagagc gtggaaacca acgatcaccc cgccgccgtg gaggacatct catggtaaag
      841 aaaaacattc ttggccgcaa gctgggccgg gacatgtgga aaagccggat gcagtttgtg
      901 gcggtcattc tgctctgtgc actgggctcc tggtgcttca gcggtctgga cgcgctgtgg
      961 cgtaccatcg acctgacggc caacacctat tttgacgagg gcaacgtggc agacctgtgg
     1021 gtcaacatga acatggatcg ggaagcgctg gagaccctgc gcaattttcc cgacgtggag
     1081 gatgtgcagg cccgcttttc cacacaggtg aaggccgacc tgccccacga gcccgtgctg
     1141 cagctggagg cctacgacgg cgtcatgcgc atcaacgttc ccaggatggt cagcggcgaa
     1201 gcgctggaac ccagcgatct gcgtggctgt cttctggacg acggctttgc ccgggccaac
     1261 gggctcgggc tgggcgataa gctgaccctg aaaactgcag cgggcgacta tgatttctgg
     1321 atccgcggca cctgccagag cgccgaatac ctgagcgaca acaaggatac cacctacgat
     1381 cctctgaact acggctttgt gctgctgtcc gccaaggcgc tgcccacgat gccgctgaat
     1441 caggccacga tcatcctgaa agaccgcagc cgcagcgacg cggtggaagc ggccatcagc
     1501 gaccgatacc cggctgcgct gatcgtcaac cgggacaccc agcgcagctc ctccgccgtg
     1561 cagtcggacg tgaccatgtt ccacaacctg tgctatctgt tcccgctgat ggcctttgcc
     1621 gtggcggcca tgatcgtgct gaccaccatc acccgcatga tggaaaacca gcggctgcag
     1681 atgggcactt tgaaggcgct tggcttccat ggccgccaaa ttcgcagcca ctatatgtgc
     1741 tacgccctct acccctcgct gatcggctcg ctgctggggc tgttcgtggg gcggcacacc
     1801 ctgcccagca tcctgtggga gctggaaatg gctcatttcc agtttcccta ccggctgaat
     1861 cctccggtgt catggtcgga atggctggtg accgggctgg gcgtgctgct ggccgtggcc
     1921 atctgctacc gcacctaccg gcagtccggc cgggaaacca ccgctcagct gctgcgcccc
     1981 aagcccccca aggcgggccg gaagctgttg ctggagcgct tcccccggct gtggagccgg
     2041 atgggcttca acgccaagat ggtcacccgc aacctgatgc gcaacaaggg ccgctccctc
     2101 acctcactgg tgggcgtgct gtgctgcacc atgctgatca tcacctccat gggcctgcag
     2161 gaaagcgtgg cctactccgt aggcaagtac tacaacggca cgctggccta caccgcccgg
     2221 gccaacctga ccggaaccac cgatcaggcg gaaagctacg gaaagcgcat cgaggcccgg
     2281 cgggtggagg cggtcatgga caagaccgtc agcatccgca ccggcgaaaa gagccgcacc
     2341 accaccctca ccgtgcttca ggacgaccag cggctgcttc tgctgggacc ggacaacgcc
     2401 tgggcgcccc tgccggagaa gggcgtggtg ctgagccaga agctggccca atatctgcgc
     2461 gccagcgtgg gcgacgtcat ccgcctctgg ctggccggag acgacgaggc cattaccacc
     2521 acagtctcgg ccattgcgcc catcgacatc ggcctgatgg ccttcatggg ccgcagccaa
     2581 tgggagggct gccggaaggg cgctttctcg cctacggccc ttcttttgct ggagcccacg
     2641 cagcgcgggc tggataccgt caacgcgctg gacgagctgg acgactggga atacccggat
     2701 cagcagtatc tggacaccat gaccgtgctg gacgcggtga ccggcgtttt caagctgatg
     2761 ttcttcatcg cgctgggact ggccttcgtg atgctgtaca acatgggcat cctgaacttc
     2821 atggagcgtt cccgggaata cgccaccctg aaggtgctgg gctatcatca gaaggagatt
     2881 cggcggctga tcaccacgga aaacgacctt ttgaccggcc taggcgtgct tctgggcgtc
     2941 ggcccgggct tctggctcac cggcgcgatt ctggaaagct gcgccagcaa gaccatggag
     3001 ttcgtgccat tcgtttcgtg gaagtccgtt ctcatcggct gcgtcgtcac ctacgccttc
     3061 tccgtcttcg tcacccgctt tctggcccgc aaggttcggg gcatcgacat ggtggcggct
     3121 ttgaagagcg tggaataaat cggaaaaaaa cgggaggaat tcaggatgaa aaagaaattg
     3181 atcagcctcg tgctttgcct gctgatgctg gcccccctct gtgcggcggg tgcggaagaa
     3241 gccgccgtct ccacggccaa cgccacagtg gacgcggcga acaccgttca actgaccgct
     3301 ccctttgcgg gcgtgctgct gccctatgac tggaacggcg gcgagcggat ctcctccggc
     3361 gacgtggtgc tggagatgga caccaacaag ctctacgccc ccgcaaacgg cacactgcag
     3421 gccgttttcg cggaggaggg cgtcc
//
LOCUS       DAWAEA010000130         3437 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4207_length_3437_cov_1.681549, whole genome shotgun sequence.
ACCESSION   DAWAEA010000130 DAWAEA010000000
VERSION     DAWAEA010000130.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4207_length_3437_cov_1.681549"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    643..728
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2058..2067
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          683 a         1003 c          972 g          683 t
ORIGIN      
        1 tcctcatcgt tcttcttcgc agccagacga atcaggtggg gcagcagatt cagcgccaca
       61 tccttaaagg ccagcgtggg accgtggaac agctccagca cgtgcacgtc cggcatcagg
      121 gtatgcagcg gcgtgacggc ggggtcgtca aaatgcttgt cgtcataggc tgcgttgacc
      181 gactgctcga tctcggcaat ggtatagtcc tccagataat cccgaaggat ccgcgccgcc
      241 tgctcctgat agctggcccc gcacagggaa gccatgcgtt cgctggaaag ctgggggaac
      301 atgctgggca catatagtcc gccatccttg gccagccccc acaaaatagc ctgagatgcg
      361 gtcacgcacg caccgccgcg ggtggaaaag aacgacatag gcacacactc ctgtctttcc
      421 ggaaaagccg gaaattctac ttcgatccaa tcctatcata caacgtcccg gcgggcggtg
      481 taaagggctg gcccgtcttt tctcagcatt ttctcatttc ttcccaagcg gcgcttcagt
      541 tactttgaac gcgcggcttc ttttctctgt ggacaaaaag tgctatgatg aatccgtacc
      601 aagcgccaaa ggcgcgggaa agggagaaga aaccatgacg cannnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnngc tattacgaag ccgtggagaa aaaacggcag gaggaaaagc gccgtaagca
      781 ggacgcgctt tttcccattg aaattacggc gctttatctg gtactgggcg cggttttcca
      841 tctttggcat ccgggttggc tgatctttct gaccattccg ttgcactata tgcaccccaa
      901 aaaccggctg gaacagctga gcaaccccat tctggtcacg ctgatctatc tggtgctggg
      961 atttttcttc aacctgtggc atccgggctg gctggtcttt ctggccatcc cgctgggcgc
     1021 aatagcgaac cactaacgaa cgaaggaaac ggggaaaaag aaaatgcgca gagaaaccat
     1081 caccaaaacc gtcaaaggcg gcattacctt cggcagcgcg ctggccatgg tcatcagcta
     1141 tgtgaactgg cactccgtgg gctgggccat ctttcacggc ctgctgagct gggtgtatgt
     1201 gatctattat ctcattcggt attaaaaagc ggcttgtaaa acccgccccg gacgatgaaa
     1261 agtccggggc gggttccttt ctaaaaatag tgaacgcgca gaaagtattg ccttctgccg
     1321 ggcaggatag aggtactaaa accttaagct gtgctccttc tccggcaaag ccatttcaag
     1381 ggacaaacgg cagcaaaagc ccgaagcctg tggtcacaaa gaatggacaa acagggaacc
     1441 ccgtcccgga taaagagaag cccgggacgg gcccttatgg agtttcagcg aatgaacgtt
     1501 gctccgtggc gctgcctagg ggccacaact ccaaaaagcg ttccacttca taggcataat
     1561 cccgctccgg gacgccttcc gccacgatca gcggggagga agccagcgtg cggccgtcca
     1621 gcatcaggcg gacttcgccg atttcgtcgc ccagctggaa cccggcgggc agttcgtccg
     1681 gcagggtgta ttccagcgta ggcacaagcc ccttccgcac cggcgcgccc agctcccgcc
     1741 cggtcacgat ggagacggtc tcccgaacac cgtcctgcac cggcaggacg cgcacgcttt
     1801 ccccggcggc aaaagccgtg aagtactcgt attccgcaaa gcccatgtcc aggatccgct
     1861 ccgcctcctg aaaccagtcg gggcagttca gcaccacgcc gatcagctcc aacccgtccc
     1921 gcttggcccc gaagaccaga cagcggcccg ccgccttggt gtagccggtc ttgatgccgg
     1981 tggccccgtc ataatcggtc agcaggcggt ttttgttgtt gaggatgcgg ggatagcttc
     2041 gtccctccca tgggatgnnn nnnnnnntgg ttcatggcct cccgggcgat cagtgccaga
     2101 tcgtaggccg tggtgtggtg gccgggggtg ggcagaccgt tgggactgag aaacacggtg
     2161 ttctggcacc caagctccgc tgcccgttcc gtcatccgac agcagaaagc gtccacgctg
     2221 ccgccgatgt gctcggcgat ggcaaccgcc gcgtcgttgc cgctggccag catcagccca
     2281 tagagcaggt cgcgcagggt gatcttttcc ccttcggaaa ggtagatgct ggtgcccggc
     2341 acaccgaagg cgtttcggct gaccgtcacc acatcgtcca gattcccttc ttccaacgcc
     2401 agcagggctg tcatgacctt ggtggtggag gccatgggca gggcggtttc ttcgttgcag
     2461 cggtagagaa cccgcccggt gtttttttcg atcagcaccg cagccttggc ggaggtctcg
     2521 atgagctttt cctcctgggc gcgggcgtgg ccgttgagga gggtcagcgc cgcgcagcag
     2581 acgaacagaa acaatcgacg gatctgttgg aaaagacgat ctttcattgc agctcgccgt
     2641 ccagaagctc atcgtcggtc tttccgaaga ttttgttttt cttcttttcg tccttggcgt
     2701 ttaattcctc gatcagctgg ggcagaagct ccaccatgcg ttccatggcg ccggttgtct
     2761 gggcgggcag cattttcacc tgcttgtccg tcaccaccag aaagcccacc ggctgcaccg
     2821 atacgcctgc gcctgtgccg cccgcaaagg ggaagatgtc cgtgggcacg ggcttttgct
     2881 tgccgtcctc tacggcgtat tcgccgccgc ccgccacaaa gccgaagctg accttggaga
     2941 tgggaatgat ggtggtgccc ttctgatcca tgaccggcgc gcctaccacc gtgtttacgt
     3001 ccaccatgcc gcggatgctc tccatggtgg tctgcatcat attgtcgatg ggatgttgca
     3061 tcgttgttcc tccttgaaaa tgagtgtggg aatgggtcag gccgcggctg ccggccgttc
     3121 gccggacgcg tactgcctgc ggctgcgcag gtagaccgaa cccatcaggg ccatcgttgg
     3181 aattagactg cccagccgca ggccgactat accctccacc agcgcttccg tgccgcttcg
     3241 ggagaaatcc gcctgtaaat gacagcggaa gacctcggga agcgcgccgg tcagccgcag
     3301 ggtctccgtc agggtgctta gggcgcagaa gcacagggcg ttcagcgccg cgtcctccat
     3361 ggaaaaacgc aggaagaccg cgcatttccg caaatggagc atccgggcaa aatgccgcag
     3421 cactgccttg tccgtca
//
LOCUS       DAWAEA010000131         3409 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4249_length_3409_cov_2.481813, whole genome shotgun sequence.
ACCESSION   DAWAEA010000131 DAWAEA010000000
VERSION     DAWAEA010000131.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4249_length_3409_cov_2.481813"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3135..3144
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          613 a         1053 c         1021 g          712 t
ORIGIN      
        1 ttccttggcc ttctccactt cctcatccgc cagacgctgg gcgctgagca aaatgctctc
       61 cagcgtggag ctggtcttgg cgatgggcgt ttctacaggc tgggcctgcg ccacgggcgc
      121 gggctttacc gcttcgcggg cggccttcag ctcgtcctga gccttctgca ggtcgctctc
      181 caaagaggtg atcttctcct gcatgttggt catttcgtcg cagatctcgt ccagaaattg
      241 gtctacatcg tcccggtcgt atccgccgcc cttgagggca aactctttgt cgtaaatatc
      301 gtcgatcgtg atggccatgt gggtcgctcc tttcatgcgt tgggctttat ggatttcgct
      361 tgtctccgaa gagaagcatt ttgaggggat aacgtccttt tcgggtgggg gagctgatct
      421 cctgaacctg cagacggccg aagccccgca cggagatcac gtcacctgct ttcacctgcg
      481 cgtctgtgcg ctgcatgggc agatgcctca atttcacctg accgccgttg atgagatcgg
      541 cggctttgga tcgggacagg cggcagccgg aggctaccac cgcgtccagc cgcgggctgg
      601 cgacggtgtc gcgccactct gttccttccg ggggagccag ttcgggcagc tccgataaca
      661 gcgccgtctg aaggcggatt cgaccggcgg agagcagact gtcccgcaga tgctctgcca
      721 tgctgtgctc gcagaacagg atggcccgct ccgcttccag cacgatgtcg ccaatgcagt
      781 gccgctccaa gcccagcccc atcacactgc ccagaatgtc ccggtgagcg ggcgcactct
      841 ggtggggcca gcggagctcc agcgcatcca tgggaaactc gggaacttca tcctccgggg
      901 aaaagcaggc gatcacccgc tctgcgtccg gatagccgcc gttcagcact gtgaagacct
      961 gcgccttttt tccggctgtt aaagcccatt gcgcttcagc cggggaaaga aagccggtga
     1021 acagcggcct gtcctgtcga aaactgcgct cggccagctc ctgaaagcgt cggcgcagca
     1081 gttcctcgtt ttgctcttct cgcatggact tacagcgccc gctgaagcat acgcagcagc
     1141 tgaagaagaa tatcgatcac cagacacacc gccagcgggg aaaagtcaaa ggaggaattc
     1201 atcagcttgg ggaaccactt gaacagaagc tgccggaagg ggttcaggat tccctccatg
     1261 tagggacgga gaaactccat cacccggctg cccggggcca cgaaggaaag cagacaatag
     1321 atcagcagcg cggtgctgac gacctgcagg aagagaatca ggataccgaa gagcgtggat
     1381 agcatggaac gcgcctcctg tgatcactgg ggatagtcca ccgcccggta gcgaccggag
     1441 acctgtccgg cagtaccggt ggcggcatag ctgcccgtga agctgcccgc cacggcggaa
     1501 gccatgctga tggggtgatt ccgctgggtg ccctgcgcga agaaccgggc cgtgggggat
     1561 tcggtgctga aggttcgggg tgcgggctgc tgcggagcct cataggaagc ggactgttgg
     1621 gcttgctgat aattttgagc gggctgctga tagctgccgc tggattgctg tgcctgctgc
     1681 tgataaccgg cggcgggctg ctgactctgc tgcgcctgcg cgtaaccggc ggagtagccc
     1741 tcctgctcgt agcgctgacg ggcgtaaccc tgggcttcag gagccgccgc agccttctgc
     1801 atggcgggat ccagcaccac cttcaccgtg ggggcggaga tgaggtaaat gccccggggc
     1861 gaaattttgt tcagcgcgca gcccagcgtg taggccgcgc cgctgagcat gtccacacag
     1921 cgctgccgct cgctgtcgct ggcgataaac tccatgttca ggaacacgga agcgttggtc
     1981 ttgatatagc cgatgacgtc cttgcattcg ttgcggctgc gaaggagcac cacatattcc
     2041 acgtgggtat aagccatgcc ttcggggttc tgctgcatcc cggggaacat gaccacgttg
     2101 ccgttcaccg ggccgtaacc ctgcgcatag ccgttggggg ccattccctg ctgtgcgggg
     2161 gctgcctgtt gagcgggcgc cgcctgctgc atgggctgct tctgcgcctg agcgcgctgc
     2221 tgggctgcgg cctgcgccgc cgcctgtttc tgctgctcgt aagcggcctg ctgctgttgg
     2281 taggcttcca tttccagatc gcgcttgctg cggtagggaa cccgtccgcc atattcgccc
     2341 tcgccgcgtc caaagggatc cagcggttcc cggcgctcgg aggtttcccc ggccgcctga
     2401 ctcatctcat cctcgatggt cgcgccgccg taagcgtcct gcgcgtcccg ggaagcataa
     2461 tagccgcttt cgtgatccgc gctgccgcga atgagccggt cactcttttc cgacagccag
     2521 ctgctcacct tctgaaagat acccaatgtt caaaaccccc ctggaatcat tgccggtgca
     2581 atgctcagtc ctgatacagc gccgtaccta cgcgcaccat ggtcgcgccc tcctgggcgg
     2641 cgatccgata atcccgggac atccccatgg aaagctcccg catttccacg ttgggcagag
     2701 cctcgtcccg aagctggtca aataaacggc gcattccgcg aaacagcccg cgcagttcgt
     2761 cctctccggc atactggggc atgatactca tcagcccttc tacatgcaga ccggaaaaat
     2821 cccgcatttt ccgaagaaac gggagaactt cctccacagg cataccgccc ttctggggct
     2881 cctgcgcaat gttgacctga accagcaccg gcagcgcccg tccccgctgc ccggcgcggc
     2941 ggtcgatctc ctgcgccaag tccatccgat ccagcgcgtg gagcaggcat acatgatcta
     3001 ttatatattt aactttgttg gtttgcaacc ttccgatcca gtgtaaatga aattccgggg
     3061 aaatttcgga aagtttgggc agggccacct gcgcccggtt ctcgccgata tcccaaattt
     3121 ccatgggctt gaggnnnnnn nnnnagcttg gcgggaggag agtcctgata acggttttcc
     3181 gctagcgttt ccagtacttt tgccacattc cggcgcagcg tttcttccgt cacactgtcc
     3241 agaagcggcg gatgaacggc cggagccgtc tgcaaatcca tggtttctcc ctccctttga
     3301 aatttggtgg atcggtcaga acagacgcac ggtcatgccg tcggtcaggt agccggttcg
     3361 gatggaggtg acgtaagcct tgccgccttc ttcccgcagc accgtcacc
//
LOCUS       DAWAEA010000132         3383 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4298_length_3383_cov_2.116587, whole genome shotgun sequence.
ACCESSION   DAWAEA010000132 DAWAEA010000000
VERSION     DAWAEA010000132.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4298_length_3383_cov_2.116587"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2619..2715
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          624 a         1084 c          964 g          614 t
ORIGIN      
        1 cccggaacag ctcgcccgcc tcgcaggcgc tgccgataat cagcccttcc cggtacttct
       61 cgatatcgaa acggggcatg ttggggtggc ggtagaaata gctgacgttg ctgtcggaga
      121 tcagatggtt caggttctgc atgcccttct gggtcttgca cagaaggatg atatgatagc
      181 tttcgcccat gctctgccct tcgatgacct gatccagatc ctgcagggta ttcgcgccct
      241 tttccgccgc gccctcatac attttaatga ggcattgagc ggtagccgcc gcgtcgtgga
      301 cggcccggtg ggcgtttttc aggctcacgc ccagctgacg gcacagcgcg cccagccggt
      361 agctcttctg gttggggtac agccgccggg cgaaggtcag cgtatccagc accggcgcgt
      421 ggaacgaaat gcccatgcgc tcgcattcgg cgttgagcat tccgatatcg aaggaagcgt
      481 tatgggccgc taccgccgcg tcgccgataa aggccagcag cttggtcacg ccctccgccg
      541 ggctttcctt gccctgcagc atcaggtcgg aaatgccggt gatctccgtg atcttgggct
      601 tcagcggcac gcccgggtcg cacaggaagc tgagctcgtc cacgatctgg ccgtccacca
      661 gcttgaccgc gcccacctcg ataatgcggt cgtggaccgt ggaaaggccg gtggtctcaa
      721 agtccagcac cacaatgggg ctggaaagga gccggtcgtc cgcgtccttg acgataggca
      781 ccatatccac cagatagccc tccacgccgg gaatgagctt aatgttgttt ttcttggccg
      841 cgccaaaggc cgacgggaac gcctgggcgg agccgtgatc cgtaatggcc acggcaggat
      901 ggccccaccg ggccgctgtg gccaccagct gggcggcttc ggcggtagcg tccatggtgg
      961 acatctggct gtgcatgtgc agctcgatgc gcttttcctc gcagttgtcc atgcgctcca
     1021 ccttgggcat ctgctgcata tcccgcacgc cgatggacag ttcacccaaa aaggtatcca
     1081 tgcggcagtc gcctcgcaga cggacccgca tgccgttctt gatctggtct acctgctcct
     1141 tcacccgctg gcgttcctcg tcggtgggcg gcacaggctc ctcgcccatg ccccggcgct
     1201 tcatgcggta ctgatagaaa gccttgcagt aaatcgtgct ggtcaggtcg taaacggcga
     1261 aggtcaccag caccgtttcg ccgcccttca gttccttggg ctcgttcacg ccggtcacgg
     1321 tgccctcgat gaccacaatg ccgctgtcct cggccagctc cacaatgtcc accggcttgt
     1381 cgccgatggc gcgtcccttc aacacgggat agcggctgtc cggcttgggc ggcggcgtat
     1441 aggccggttt cggtgcggga gtcgctttgg gcttgggctt gggtgcgggg gccgaagcga
     1501 ccggcgctgc gccggggatc tccatagcgg cggcctgcgc gtccagcgcc gcgtcgtccc
     1561 acgggggcgg ctcttccatg ctcccggatg cgccggaacc gtcgaagccg ccggccagcg
     1621 cgggcgagaa ggagaagccc cgctcctccc gcattttctt cgtccaggct tcccgctcgc
     1681 cgcagactgt cagacccacc gtgaccttca cccggaaaat atcgtagatg cactgggcca
     1741 cccgcttatc cagctgatgg gaggcaaaat actgcatgga gatattatcc gggcaggtca
     1801 gcaggatccg tccgttttcc atgctccagc ccacgtcgtc cagccacgcc agcagcgccg
     1861 ggctctggcg gcgcagaaaa tccgtcagca cggacttata cttgtccaaa tgaccgagaa
     1921 agtcctctcc cagccccggg ctggcgatac gcagggctac gttcacctgc ggaaaaagct
     1981 ccttcagggt gcgctccacc tgcaggaaat cattttcccg caccagttcg tcgcacagga
     2041 aactcatata ggccttattg gccgcttttt tgtacaggac ccgctcgatg tgcagctttc
     2101 cctgcaggga gggcaccgcc gcctccaggg cctgcagcat ttccgttttg ttcattcgtt
     2161 cgttcccttt ccgttttcag gggggtgagc cagtaaaaga ccgatcagcg gcagcgcgcc
     2221 caatgccagc ggcaaagcgt agcgcagata cgcgcaaggg gcaaaccgaa cagaagcgct
     2281 ccccggttcc gccgaaaaac cgccagccac agcgccccga agaacagcca gcaccagaag
     2341 gccggttcga tgagggcgga caccagcggg atccgccggt attcgttttc cacaaacaac
     2401 cggtccagcg tttccatcaa tcccggccac aaagaggcgc ggctgacgcc gtaaccgggc
     2461 tgagcggcgg tctccatgta gccgtgtccg ccccgctccg catagatgtc cagatgggtg
     2521 gtatcgtcca ggggccagta gcccttggtg agatacgccc atgcatccgc gaaatccgca
     2581 ggatattgcc gcaaaagtcg cccgtacagc ttcaaaaann nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnntcagt tcttcctgct gccggacgta cacccgggag acctgcgcca
     2761 gcggcacgct gagcagctcc cgcagaccgg tcttgctggg atgcagcgcc gccttcatgc
     2821 cctgattggc cccggcaaat ccgattatcc cgcacagaag cagcactgcg aaacgcctgt
     2881 gcccttcttt gggcagaaga aaccagccgg agaccagcgc gatcacaacg gctgccgctc
     2941 cgttgggccg caagaggcag ctcaaagccg tcaaagcaac gactgcaacg gcatagcccc
     3001 gctttttcag ccgtttcggc tcccgctgcc cctccgccaa caggcaaaga aggggcaaca
     3061 gagccgcgca gaaaaacaag tccttggtgg tggacatcac cagcagcgga aatccgggca
     3121 gcagaccgta ggccgtcgcc agcccgatac gaaagccgcg ttttacaccc agacggcaca
     3181 gcattgcgtt ggcataagcc agcgcagccg ccaccgcaca ggcctgcact gccgtataca
     3241 gggcgatccc cgcgttatag ctgcccatcg cgccgcccag ccggtaaaac gccgccagca
     3301 gcagcgtatg cagcggcgga aaagctccat tgtactgtcc tgtggtaaac tgcgccgctt
     3361 cccccgctga atcataattg aac
//
LOCUS       DAWAEA010000133         3378 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4303_length_3378_cov_2.338850, whole genome shotgun sequence.
ACCESSION   DAWAEA010000133 DAWAEA010000000
VERSION     DAWAEA010000133.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4303_length_3378_cov_2.338850"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    896..905
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1699..1750
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2659..2668
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          665 a          975 c          965 g          701 t
ORIGIN      
        1 gcttccttgt tagttgaata ggaaagaccg ccattcggcg acgggcctca tggctcgcaa
       61 caaaggccag cgtggatttg actccacgct ggcctttcgc attgcgaaaa tagccaagat
      121 acggtgaaac ttctcaagca tcattggccg agtaagcaca ttgccaacct cgaattggaa
      181 aacaagtctg cttatggatg tagcgaagcc gtccaacctg cgccgtaaca tctcaacccg
      241 catattttta agtctgcctc acgccaactg cgggggagca gagcggcgat aggcagcgga
      301 gggaaaaagg cccgcccatt ttgacggcgg aaaaaggcga gcacgagcgc gctccaagcg
      361 taagtgaaag ctagtcaacc tcatcgcgcg cgacattgcg agccccgccg tcaaaatggg
      421 cagtagaagc ctttttcccg tagtgccgtg ccgctggctt tcttttgcac acttttcttt
      481 cgccatcgaa agaaaagtgt gagcgcccca tgggacggca agtcccactc ccgcgccgta
      541 gtcggcgcgt cataccccac ccggggcaag gggtatgcgc ctgcgggcgc accaaagggc
      601 tttccgttcg cactttgggc acctttggac actgaagttg gcgagactcc cccgccgtag
      661 tcggtgtgcc aagcccccca gcagcgaatc ttcttacggc atagttgtcg tacaccgatg
      721 caggcagggg gctttcttcg gggcaaacga tcggcgcgga gcgcacggca gtgcgatccg
      781 cttgttggtt acctgaactt tagattgctg tctgccatcg gcagtcatac ccgccgggtt
      841 cacgattttc ttcggctccc tcgccgaaaa taccaacagc cccgccacgg cggcgnnnnn
      901 nnnnnaaaat aaaaccttcc gccccttaat cggagcggaa ggttttcaaa tctgtttttt
      961 caggcttgcg ccagcggctc aaaatccgca acgccgtgct tgcacagcgg gcagatgaag
     1021 tcgtccggca gcacgtcgcc ctcgtagatg tagccgcaga ccttgcagac gaagccggtc
     1081 ttgccctccg tctggggctt gggcttgaca tgcttctggt agtaggtgta ggtcatggtc
     1141 tcccggtcgc tcatcacccg cgcttcggtg acggagcaga tgaacattcc gtgggtgccc
     1201 agatccacat actgctccac cttcagggaa aacgccgcgt tgatgtactt gggcaggatg
     1261 gccagcccgt tgtccgaacg gatgggcgcc atgccggcaa atttgtccgc cgcgcggccg
     1321 ctctggaagc cgaaggtctc aaagaccttg aagggcgcgt ccacggacag gcagttcacg
     1381 ttgagaatgc cggtctgctt gatgacgtga tgggaatagt tggccttgtt gatgttgacg
     1441 gcaatacggt tgggattgtc ggaaacctgc gtgacggtgt tcacgatcag gccgttgtcc
     1501 cgcttgccgt cgttggacgt gaccacgtat agaccatagc cgatgcggaa cagggcgctc
     1561 atgtcgtgct tgtcggccat ttcgctgtcc cgggccgcat attcccggca aagctccttc
     1621 gccagctcgc ccagctgctc ccggttttca gcggacagcg cggaacggat ggtcaccggc
     1681 gtgcccacga atgtgatgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn ttttcgatca gctccaccgt gcggttctgg tagttccgct cggtcagatg
     1801 ctcgataaag gtgcgcatga aggggaacac atcggcgttg taggtggtgg tggccagcac
     1861 cagctttccg tagcggaatg cgtctgccac agcctgcgcc atgtcgcacc gggccagatc
     1921 gtgcacgacg accttcgggc agccgcccag acggagcttt tcggccagca gctcggcggc
     1981 cttgccggtg tggccgtaaa cggaggtgta ggcgatcatc acgccgtcgc tctccaccgc
     2041 ataggaagac caggtgttgt acagctccag cgcttcggcc agattgtcct ccaccaccgg
     2101 gccgtgcagg gggcagatct tttcgatatc cagcgctgcg gccttcttga gcaggttctg
     2161 cacctgcgcg ccgtacttgc ccacgatgcc gatatagtag cgccgggcct cgtccgccca
     2221 gtcctcgctc acgtccagcg cgccgaactt gccgaaaccg tcggcggaga acagcacctt
     2281 ttcggtggat tcatagctga ccatgacctc cggccagtgc accatgggcg cgaacacgaa
     2341 ggtcagggta tgtttgccca gtgaaaggga ctcgccgtcc ttcaccacca ccttgttttc
     2401 gcacaggcca gggccgaaga actggtcgat catggcgaag gtcttggcgt tggccaccac
     2461 cgccgcctcc gggtaggcgc tcagaaagct ggcgatattg gcggaatggt cgggctccat
     2521 atgctgcacc accagatagt ccggcttccg cccgcccaga atcagggcca gattgtccag
     2581 ccattcatgc tgaaaatgcc ggtctaccgt gtccatcacg gcgatcttgt catctagaat
     2641 cacgtaagaa ttataggcnn nnnnnnnngt atactgaccc tcgaacaaat ccacctgatg
     2701 gtcgtttacg cctgcgtaaa gaatatcctt ggtgatgaac ataagccgca tcctttctgt
     2761 tctatgggag tttccggggg gaacagcccg cccggaaagt gcatctttgg ggtacaagta
     2821 aatgataccc atttttccga aaagtgaaac cattttttcc acagttcctg cactttttcc
     2881 attgcccaaa gcactgcgtt cgccatctgt ccgcgcattg acagagggcg tttcttcctc
     2941 tacaataggt tggatgaaat gtgatgcgat aaaggaggct ttccctttga atattttgat
     3001 caccggggct cggggcttta tgggcaaaaa cctgcgttcc gccctgacgg gccggtacgg
     3061 agacgcgcac cggctgatgc tgctggacat gccccacacg gaggaagaac tgctggccgc
     3121 cgcggcggaa gcggatttcg tctttcatct ggcgggcgtg aaccggccca cggacccggc
     3181 ggattttcag aaagggaacg ccgattttac ccggcagctg ctgacgctgc tgaaagagcg
     3241 cggcaagcgc ccaccggtgc tgctcagctc gtccattcag gcggcgctgg aaaatcccta
     3301 tggtcagagc aaattgagcg ccgaacaggc cgtggcggac tatggccggg agacgggcgc
     3361 ggcggtgtat ctttaccg
//
LOCUS       DAWAEA010000134         3356 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4341_length_3356_cov_1.974553, whole genome shotgun sequence.
ACCESSION   DAWAEA010000134 DAWAEA010000000
VERSION     DAWAEA010000134.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4341_length_3356_cov_1.974553"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2915..2924
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          620 a         1046 c          968 g          712 t
ORIGIN      
        1 ttttcgtttt ggttgacggc tgtcagccgc ccgattggat acaaaaaagc ccccggagaa
       61 aggcaagccc catggcttct tcctccgggg gattcgtttg tgggtcagtt tttttcttcc
      121 gaagcccgaa gtgcttcttc aacggccttg cgccagtcgt tgtcctgctc caggggttcc
      181 atttcttctt ccacaggcag ctccgccgga atttctggat cctccgcagg agtgggggtg
      241 ggctcgtcca gattgctgga aatggccgga cggttcgcct tgccgcgctt gctcctggtc
      301 tcctgattct tgggcttgtt ctccgggttc tgctggggtt tctgccactt gatctcctcc
      361 accgggaagg tcttgagttc cgtggtgtcg cccttggtca ggcggacggt gacagtctcc
      421 ttcagaatgt tcaggtcggt cacggtgcca acgccttcgg gcgtttccgc ttccttgccc
      481 agcttgggca tcttcttgcg ggtggcctcg taatggtctt cctcaaactt gaggcagcac
      541 atgagccgtc cgcacacgcc gctgatcttg gtggggttca gggaaagatt ctgctccttg
      601 gccatcttga tgctgaccgg ctggaaatcc cccagaaagc tgccgcagca gatgggccgt
      661 ccgcaggggc ccaacccgcc tagcatcttg gcctcgtccc gcacgccgat ctgccgcagc
      721 tcgatgcggg tgtggaaaac ggaagccaag tcctttacca gcgaacggaa gtccacccgg
      781 ccgttggcag tgaagtaaaa gaggatcttg gtgttgtcga aggtctcttc cacgcctacc
      841 agcttcatat ccagcttgtg ctcttcgatc ttccgctggc agattttgaa cgcgtccttt
      901 tcgaaggcct cgttgtccgc cgcgtggcgc gcgtcctgag gggtggccac ccgcaccacc
      961 tgcttgaggg gggaggcgat caggtcgtcg tccacctgac gcacgccggt aataacctga
     1021 ccgtattcca gaccccgggc cgtttccacg atcacgaagt ccccggccac gggccagagc
     1081 cggccgggat caaaatagta cagctttccg gcatttttga accggacgcc tacgattgcc
     1141 gccatgtttt ttgttcctcc aatattgtca tcatcagctg atccacggtg gactgccagt
     1201 tcacctgacc ggcccggcgc ttccgcgcca gaaagaccgc gtccagcatt cggttcaggc
     1261 ccgcttcgtc cgcctgagga agacaggccc gccacgcggc gggatactgg gccagcgccc
     1321 cttcgggcag aagccccgcc ttggcgagca tcgcctgatg cagggcgttt tccagtaaga
     1381 tcaggtattg atcggcagtt tcccgctgat cctttaaccg ggtggacacc ttcaatacgt
     1441 ctgcgtcgct caggagggac agcgcgtccg ttaaaaattc ccggtctttt tcctccagcc
     1501 cgtcgccgtt cagcacttcc agcgccatgc cgatgttgcc gcccgccagc cccaccgccg
     1561 tggggatccg ggcggggtca cagcccatcc gggtcagcgt ttccgtcatc agctcgtccg
     1621 gccagggacg catcttgatc cgggcgcagc gggaagcaat ggtgcccagc gtggctgtca
     1681 gttcgtgggt catcagcaga aagaccaccc gcgctaccgg ctcctccagc gatttcagca
     1741 gacagttctg ggcctgcggg gtcatttttt ccaccggctc gatcagcacc acccggtagc
     1801 cggtgccgaa ggaatgctgc cccaccgcgc ggatcacgtc gcgcacccgg tctacgccga
     1861 tctgcttgcc gtcgtccgca aacagggaga tcacgtccgg ctcgttgtcg tcccagaccc
     1921 tccggcagcc ctcgcagacg ccgcagggct tttcgtcggt ttcggacgta caaaacaggg
     1981 cgctggccag cagcctggca aaagttcgtt tgccaagccc ccgagcgccc gtcaggagat
     2041 atgcgtgaac catttccccg gtttgaaact ggtgaagcgc cgttttacag ctggcgctca
     2101 ggcggccgta atccgtaaag accggccgcc ccgcctcccg tggtatctgt gccaagctct
     2161 ttccgtctcc ttacagcttc acaaactgat ccaccgtgag cacgaaaata gtcgcgccgc
     2221 ctactaccac ttcaatggga tagggggtgt aggaccccgg catacccacc atggacgcgg
     2281 gcgagggcgc gatctgcttg cggctcttgc aaaccttttc gatcagatcc atggcctcct
     2341 gaaaacgatc gtcctctacg cctaccagca gggtggtgtt gcccgcccgc agaaagccgc
     2401 cggtggtggc cagcttggtg acgccgaagc cgtcgttcat cagttgtccg atcaaacggg
     2461 aagaatcttc atcctgtacg atagcgatga tcagtttcat cctcgttcct cctctttttg
     2521 gttgcccgat tttccatttc agtataccgc aaatctttcc tgcggttgac gggcgcggcc
     2581 cgccatgcta caataatact ggtgttttgg gcttttcaga ggggaaatcc cttgcaccgg
     2641 cccgagttat cctgtacaat aacagggttc gggaaagaaa ttcaactttt acaaagaagg
     2701 agcgtgaccg ccgtgcgggt ctccaaaaag gccattctgg cccagctgga aaagacctat
     2761 ccggacgcga ggccggagct gaacttccgc aatccctatg aaacgctggt ggcggtgatg
     2821 ctctccgccc agtgcaccga caagcaggtg aacaaggtca cgccggcgct gttcgaacgc
     2881 tatcccgatg tggaaagcat ggccgccgcc agcgnnnnnn nnnntgcccg gggatatgga
     2941 cgcgctgcaa accctgcccg gcgtggggcg gaagaccgcc aacgtggtgc tggccaacgc
     3001 gttcggcgtg cccaccattg cggtggacac ccatgtgttc cgggtcagca accgtctggg
     3061 gctggcggat gcaactacgg tggaaaaaaa ggaggagcag ctgatgaagg ccattcccaa
     3121 acaggattgg gtggccgccc atcactggct gatcttccat ggccgcagag tgtgccacgc
     3181 ccgaaacccg gagtgtgagc gctgcaccct gcgggaatac tgcaaggcgt attacgcggg
     3241 cgatacgacc ggcccgaagc agaagcaggc ggcccggaaa agcctgaacg cctgacgaaa
     3301 ccctgacgga ggaaaaaaca tggcatcatt tgtagatcgc gttaccatta ccgcga
//
LOCUS       DAWAEA010000135         3352 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4349_length_3352_cov_2.087352, whole genome shotgun sequence.
ACCESSION   DAWAEA010000135 DAWAEA010000000
VERSION     DAWAEA010000135.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4349_length_3352_cov_2.087352"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          544 a         1131 c          957 g          720 t
ORIGIN      
        1 cgatgggtgc ccacgatcat gtccacttcg cccttttcca gctttttcag ggtctccttc
       61 tgctcggcgg cggtgcggaa ccggctgagc atctccaccc gcacgtcgaa gccggaaaag
      121 cgcttcttga tggtgttgta gtgctgctgc accagaatgg tggtaggggc cagcagggcc
      181 acctgcttgc cctccaccac ggccttgaag gcggcccgca gggctacctc cgtcttgccg
      241 taccccacgt cgccgcacaa aagccgatcc atgttgtgag ggctttccat gtcggcctcg
      301 atctgggcca cggactgctg ctgatcctgc gtcagctcgt aggggaaggc gtcctcgaac
      361 tcctgctgcc acggattttc gtggctgaag gcgtagccgg ggtttttggc ccggcgggcg
      421 tacagcgccg ccagatcgaa ggccagcttt ttcagccccg ccttcacctt gctcttttga
      481 cgctcccact cgccgccgcc cagatggttc agcttgggca ggctgtgctc cgcgccgatg
      541 aacttctgca cccggtcgaa ctggtaggcg ggcacgtaga gcttgtcgtt tccggcgtac
      601 tgaatttgca gatagtcccg ggtcacgccg tcgcccttca tctgggtcac gcccagataa
      661 acgcctacgc cgtgcaggtc gtgcaccacg taatcgccgg gcttcaggtc ggtaaagctg
      721 gaaatccgct cccccgcgtt ctgcttcttc cgggcccggt gataggcgga gccgaaaata
      781 tccgtatcgc tgagcacgca caggtgcgcg ttcgggtgca gaaagccctt gttataggag
      841 accggcagca gcaccacttc ccgcaggatg atgttgccgt ccagcgattc gcagtacacg
      901 gaggggatgt tcatttcctc tagggttttc tgcagcctgc ggccccggtt ctcgccgccg
      961 gtcagcagga tgatggcgca gttctgctcc ttccacgccc tgatgtcctt gcccagtgcg
     1021 gccagctggt tctgataggg catgggcggc ttggagccga aggcgatcag cttatccggc
     1081 agaagccgcc ccagcgaggt ctgcacgtta tcgctcagcg ataccacggg ccgcttttcc
     1141 cagtcggcca gcagctcgtc ataggagaag cgcaggtttt tttgctcgtc gaaggcgtcg
     1201 cccctcagcc gcgcgtcctc gtaggcctcg acaaattcct cctgcaacac cttcaggcgc
     1261 ccggcgtact gctcgggccg gtcgcacaaa agaatgggat cgtccagata atccagcgcg
     1321 gaaaccgtct gttcgcacag cacgttcatc cacatgggcg cggtgcgcag ggtctgtccc
     1381 gcttccacca gcgcggcttc gtccagatgg cgacgctcgt cgggcacgtc cttccggaag
     1441 gcctccgccg tttcttcctc cgggcgggct tccttcgcag ctttggccgt cttgcccagc
     1501 ttggcaggtt tgccgtccag caggtcttcg ctgagctcca gcgcgtccag ttcggaaagg
     1561 aactcatcca gtgccggcag cgtatcgccg ttatccagcg tggtctcccg cgcctgaggc
     1621 tcctccgcct gcgccttctc ccccgcgccg gtctcgatgg cccggcgaag ccgctccgcc
     1681 gccgcttccc ggtcggtcac caggcactcg gtggccgggg cgatacgggc cgtggacagc
     1741 cgctcgatgc tccgctgact gatgcagtcg aaccggcgga tgctgtccac ttgcgtgtca
     1801 aaaaactcga tgcgcagggc gttgccctcg ttgggcggga acacgtccag aatggagccc
     1861 cgcatggcgc actggccgca gccctccacc atggagaccc gctcgtagcc gctggccagc
     1921 aggcggttca tcagttcctc cgggggataa tcccggcctt cctccagcgc gattacgctc
     1981 tggcggaaaa ccgccttcgg cacgcagcgg tccagcaggc tttccaggct gaggcacaga
     2041 atgcgcgcgt ttttccggca aagctcatcc agattgcgca gccgcagcca cgtgctttcc
     2101 cggctggacg cggcgcggct gaactgcaga tccctcggcg gcagcacgcc gcatttttcc
     2161 ccggtcagcc gctcaatgtc ctgcgcatag cgctgggcca ccagctccgt ggagcaaagc
     2221 agcaccaccg ggcggccggt cttgcgccgc agcgccgccg ccagaaaggg ccgttcgccc
     2281 tcagcgattt cataaacgga cgtacagccc gcccggccca gccgttccac cagcgttttc
     2341 cactcgtccc ggctttcgta ggcgctgaaa agcccctcgt tcattctttt ccttcctccg
     2401 cctgctccga agccgccgcg tcgtccccct ccggggccgc tttcttcgtt gacttctcct
     2461 tctttggctt cttggtatta tagcggttca tagcgctgtt aatcccgttt ttcagatatt
     2521 ccagcaccgc gtcggcggcc tgctcgtagg ccttgtcggc gatctcctgt tcctccgccg
     2581 tctgataatg actcaacacc cagtccgcca gattccagcc gaggccccgc tggcccaccc
     2641 ccacgcgaat gcgggggaaa tcctccgtgc cggtctccgc cacgatggaa cgcattccgt
     2701 tgtgggtgcc tgcgctgccg ttgggccgga tgcgcactgt gccctgcgga atgtcgatat
     2761 cgtcatagat caccagcacg ttttccggcg ggattttata gtagtccatc agttccttca
     2821 cggccagacc gctcaggttc ataaaggtct ggggcagggc caaaatcacc ttctgcccgt
     2881 ccacgaagca gtcggcggtc tgggaatagc ccttttcccg gctgatagtg acccccagct
     2941 ttttttccag cacggccata acgtcaaagc ctacgttatg ccgggtatgg gcatactgct
     3001 ggccgggatt gcccagcccg acgatcagat acatcgttcc cgttccccct ttacggacaa
     3061 acacgttcgg cggggactgc attgccgccc ccgctttttc gcagctattg tagcatgaaa
     3121 gcgtgctttc ggcaagggaa agcggccctt cctccgctga aaaagcgtct cccccatgct
     3181 tgctctttgg ggagacgctt cctttattca atttactgca aaataagcct tttttagcag
     3241 ctgtgcgcca ttcagcgggg ccagcttact tttcccgctt gaactgccgg aaagaaaagt
     3301 cgccgatcag aagcggaaac aggacttgtc cgtaattcac gccgccccag cc
//
LOCUS       DAWAEA010000136         3337 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4377_length_3337_cov_2.436929, whole genome shotgun sequence.
ACCESSION   DAWAEA010000136 DAWAEA010000000
VERSION     DAWAEA010000136.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4377_length_3337_cov_2.436929"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3041..3108
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          653 a         1099 c          893 g          624 t
ORIGIN      
        1 gttgtactgt cctgtggtaa actgcgccgc ttcccccgct gaatcataat tgaacatacc
       61 gggaaaatag gccagataat agggcagcca gcacacaagc agcgccgcaa acgtcatcca
      121 gaacgtccgt ccaggcgtgg acgcgtcttc tttcgaagcg gcggcagcgc cagccgttcc
      181 cttcgccagc aagaggaaca agccccctgc cgcgggagca caggcaagac tcgccgccag
      241 caacagcaat ccgccactct gcaccgttcc ggcgctgtcc agccgcgccc ccagcagctg
      301 gcaaaaggcg aaaatcagcc cgaacgccag cgaaaaccgc agaagccgcc tgtctttctc
      361 cgaaagaacc gcttttccag ccgcccacag cagcccggcc aacagcagca tggccgccga
      421 atgggtcgta tcggcgctcc agcggagcag gcaggacaaa agacctgcag ccgccagcgc
      481 acaggccgcc gctccaaccc cggccctttt ccccactgct tccagcctca ttccgttttt
      541 ccttcccgtt cccgggcgat ctcccgggcc cgggcgatca gctgtcccgc cagatcgccc
      601 tcgatgcgcc ggggtgcttt tcctttttca aacagcgcgc ccacggcgta ggctccgccc
      661 tcgcctccgg cgttctccgc cgcgctcagg ccgccgccgg ccagccccac gtccgcctcc
      721 cgggcctcgc cggggccgtt gaccacacag cccatcaccg ccacggtcat gggcacgcgc
      781 atgtcggaaa gttcttcctg caccctgcgg gcgatggccg ccacgtcaat gccggtgcgg
      841 ccgcaggtgg ggcagctgac cacgttgacg tagctcttcc gcaatccgca ggcccgcagg
      901 atgtcaatgg ccgcctgagc ttcagggatg gggctgcccg tcagagatac gcgcaccgta
      961 tcgccaatgc cctgcaaaag cagcgccccg atgccgatgg cgcttttgat attccccgcc
     1021 ccgggcagac ccgcttccgt cacgcccaca tgcaggggat aatccgtctc cttcgccgcc
     1081 agctcgtagg ccgctaccgt cagcggcaca ctgctggcct tcagggacag cacaatatca
     1141 tgaaaaccgg cttcttccag cagacgggca tggctcaggg cgctgtccag cattccccgg
     1201 gcggtcacgc cgccctcccg gcgcagaata tccttctcga tgctgccgga gttcacgccg
     1261 atgcggatgg gcacatgatg cattcgggcg cagtccgcca cccggcgcac cttctccata
     1321 ccgccgatat tgccggggtt gatgcgcacc ttggctacgc cccgctcgat ggcccccacc
     1381 gccagatcgg cccggaagtg gatatccgcc accaacggca ccggcgaacc gtccacaatg
     1441 gcgggcagcg ctttcaggca gtcctcatcg tacacgctca gacgcacgat gtcgcacccg
     1501 gcctgcgcca gctcccgaat ctgctgcagc gtggccgcag cgtcccggct gtccgtattg
     1561 gtcatgctct gcacgctcac cggcgcgccg ccgccaatgg gcacttttcc aatgaaaaaa
     1621 gaacgggtag cgctcatgca aaaaccgcct ttcaaatgca aaatatccat aactcaatga
     1681 agggaacggc ataccgccaa gcggggcagg ccgcatcaag ggtgcgcagc accgagggtt
     1741 gaggacaccg cgcagcggta ccgcaaaccc gaagcctgcg acgcgcagcg gggcaggccg
     1801 catcaagggt gcgcagcacc gaggacgagc acgccgcgca gcggcagcgc agtccgaagc
     1861 ctgcgacgcg cagcggggca ggccgcaatg attataacat aaatcgcttc ccaaaggaaa
     1921 gagacagccc ctccatggag gaaacggaaa aaatattgtc caactttttc ccagcgttga
     1981 caggcccggt tcaccgtgct atgctaacaa tcgtatcctg tacataaagt ggaggcctat
     2041 gcggtttccc tgacggaggc ggatatgcac cagagccgtt tttaccgcac acagctgacg
     2101 cggctgatct ttcaggcgct gttatatatt gggttgttcg tttctttttt tggacttttg
     2161 tccatcagca atccccagct tttgaatttg agccgtaccg cggccaccac catgggtgtg
     2221 tttgcggtat gtctttttgg gttgaccatg gtgtacggcg gctttgagat cggccgcaag
     2281 ctcaagcgtt ccgtctttgc cagcctgtcg ctgaccactt ttttcacgga cgcgatctcc
     2341 tacctttttt tgcagatcat gaacgtgaat cctcaaaacc cggaggccaa cgccacgctg
     2401 accctgtggg gcgaggattt ttatctgctg atgtgtgcct ttgcgctgca gatccttttg
     2461 atttacctca ttatttcgct ggcctatttc ttctatttcc gcatcaaccc gccccggcgc
     2521 tgtctgatcg gcacctcctc gcaggcgttg gcggagcatg tggcggtcaa gctgaaatcc
     2581 ttcccccagc gctaccggct cagcgaagtg atccactacc agtgcccgga cgtacacgag
     2641 accattctgg agcacgacac catctttctg gcgggcgtgc ccgacacgga ggaaggcgca
     2701 ctggaagcct tctgttatca gtacaacaag agtatgtacc tcatggccga gctggaggat
     2761 gtgatcatct ccaccgcgga aagtaccgtg ctggacgaca cgccctttct gcacattcac
     2821 cgcacggaaa tgacgctgat gcagcgtttc ctaaaacgtg ccttcgatat tgttttcagc
     2881 ctggcggggc tgatcctgct cagtcccatt ctgctggcga cggcggcggc gctgaagctg
     2941 gcgggcaacg gttccatctt cttccggcag aagcgggcta ccatcaacgg caacgtcttt
     3001 gagatcatca aattccgcac catgtacgaa gacgatcccc nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat gtggaaaagt
     3121 acacccggga agtgccggag ttccgctacc gccagcgcat gaaagccggg ctgaccgggc
     3181 tggcccagat cgagggcaag tacaacacct cccccaagga caaggccatt ctggacctgc
     3241 tctacatcga aaacttcacc ctgagctacg atttcaagct gcttctgcgc acgtttacca
     3301 ttttcttccg ccgggacagc accgagggct tcggcga
//
LOCUS       DAWAEA010000137         3296 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4424_length_3296_cov_1.848812, whole genome shotgun sequence.
ACCESSION   DAWAEA010000137 DAWAEA010000000
VERSION     DAWAEA010000137.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4424_length_3296_cov_1.848812"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    232..490
                     /estimated_length=259
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          606 a         1018 c          831 g          582 t
ORIGIN      
        1 accacgaaca gagccgtcat acaggagtcg atgccttcca gattccaagg gagaatctgc
       61 ccaagcgccg cgcccagcag cgtgcccaat atccaatagg catagctgag cactgccacg
      121 aggaacagca cattcttccg ttcgggcagg tcgcggggca gactgcagtc caccgcgtag
      181 gtttcgtccg tcagcgtatg gaccatcagc ggcgctttct ttcccatgct gnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn ccacaaagct cagggcgtag aacgtctggc ggctgttcat cagcagcgcc
      541 gtcacggcta cggtcatcag ggaagctccg cccgccatca gggacaccag cacaaactgg
      601 aacgcgcccg tatacaccgc cacgctggac aaaagcgaca ggtaccacgg atagcccgcg
      661 tccgacatgg tgatgccgaa cgcgatcgca ataaaaacat agctgagcaa aatcggcagt
      721 gattctctca gcgctatttt ccagacttcc ttcttcatcg ccctgcaccc tttcgtaatc
      781 ccatcctcgt tgacgggaca gcgccacctt agcacagggc cggggcggat agcaatgaca
      841 gaagcgggct gtttttttcc gaaaagcaaa aaggacggag cgcatcgtcc gtcctcgttt
      901 atctcaaaag cgaaggggca ggaatcgacg cctccggccg aagtttttag agacagaagc
      961 tttcatagca ttttcctgcg aaccagtttc cgccccttta aggtcacttc gcagcctgcc
     1021 agaaaaacag ccgtccacgt ccttcgttca ggctcttttg cagactgggt tcttcgccgc
     1081 ttaaaagcag ccgtccatcc gtttccccgt tcagcagaac ggtaacgggc agactttgct
     1141 cgccggcgga aaacacgcag cggtattttc cggctggaag gccctgcttt tcctcgtcca
     1201 gcaaacggag cgaccactcg ttttctccgt gggtcagttc gacccggcgg ctgtagggcc
     1261 tgcccagcag cgcccgggcc ccggaaacgt cgccctgagc cagcttctgc cggatggcgg
     1321 tagagctgac ctcctgcccg tgcagggtga tgggcggcac cacgctgacc tcgaagccca
     1381 gcgccgctcc caatgcctgc agcagcgccg gtgaaccact tccgcctgcg ccgaagctgt
     1441 gattataacc gcaaaccacc gccttggggt gcagacggcg cactagttcg cccacatagc
     1501 attccggcgg ctgatcccgc atctgctcgt caaagggcat agcaaagaaa acgtccgccc
     1561 cggcatgaga gatcagagag atccgttcct ccagcgtggt cagcagccgg ggagcctttt
     1621 cgggggcgat cagctccata gggtgccggg caaaggtgca gaccaccacc ggcgcgccgt
     1681 agcgcatatg cagcgtccgc gcccgttcca gcagcacctg atggccgata tgcacgccgt
     1741 cgaacatgcc cagcgccagc acgctggatt cttcgctgtg tttaccgttc agaatgactt
     1801 tcatcttgat cccctcactc gcccagccag gtgcgaatct tccattcatg gttctgccga
     1861 cgggctacgc ccatcagttc gcccgacagc cacagacagg cgtcgccgcc ctccgggact
     1921 ttttctccgc cctcaaaggc agaaaccggc agcatgccgc cgttgcgcag aggcttttcc
     1981 agccgggcgg gcgtttccag ccacggcaaa tgccgcagaa ccgtctccgg cggcagcagc
     2041 agcgtttcga ggtcgtcgct ttctttggcc tgacggagcc gttccagcgt gaccgccgtc
     2101 tgcagagtaa aggccccact ttccgcccgc agcaaaaagc gcatatgggc gggacagttc
     2161 agcgcttcgc ccagatcgtt gcacagagtg cgaacgtaga agcccttgga gcagtgtact
     2221 ttcagcagcg cgccgtggtt ctccgtcatg ctctgcagtt ccagtgaatg taccgtgacc
     2281 tcccgcttgg gaatttccgc cgtccgcccc ttccgcgcca gctcatacag tcgctgcccg
     2341 tcctgcttaa gcgcgctgta catgggcgga cgctgccaca gctgccccgt aaaggagaaa
     2401 atcacttccc gcagggccgc ctcgtccggg taattttccc cggatgcgat cacccgcccc
     2461 tgcgcgtcct gcgtgtcggt ggccgcgccg aaagcgacct ccgtcacata gaccttttcc
     2521 ttctcctgca tgtagtcgaa cagcggcgcc gccttgccga tcatcagagg cagcaccccg
     2581 gaggcctccg ggtcgagggt gccgccgtga ccgaccttgg ctccattcag cagccagcgc
     2641 acatagccga ccacctgcga ggaggtcatt cccggcggtt tcagaatatt cagaaagccg
     2701 cagagaggct ttttcgcttc actcatgagt ttttttgctc ctccaacgcc tgatgcagcg
     2761 ccgtttcgac ttccctcaca gcctgtgcag cgggcatacg cagcgtgcag cccgccgcct
     2821 gcggatggcc gccgccgccg aatcgccggg caacctgatc cacccgatag ggcggcagtg
     2881 cccggaagct gatcttcgtc acgttttctt cctgctcgtg cagataatag gccatgcaaa
     2941 cgccctgaat gtcgatggca taatccacca cgccttcggt ttccaccggg ttcaggccgt
     3001 tttgacgaaa ctgttccagc gacgtgcaca gccctgcaat acagccgccc tccgttaccc
     3061 gcagagacgg cagcgtcacg cccagcagac ggacgaaatc ctcccgcttt tcacggaaca
     3121 gaacgcggct gatctccgca aggggcagcc cggcctccat cagcccggcc atcatttcaa
     3181 agcattcgcc gttcacgctg tcgtatacaa aattgcccgt gtcggtagac agggccgtgt
     3241 acaggcagac ggccgcttcc cgtgaaagag ggacttccag agcacggtac aaccgg
//
LOCUS       DAWAEA010000138         3272 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4460_length_3272_cov_1.980727, whole genome shotgun sequence.
ACCESSION   DAWAEA010000138 DAWAEA010000000
VERSION     DAWAEA010000138.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4460_length_3272_cov_1.980727"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2401..2410
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          755 a          883 c         1150 g          474 t
ORIGIN      
        1 atcgtgaccg aagcgggaaa gatcagcccc gagccggagt tccctgaccg ggaaagactg
       61 aaacagtacc agacggacgc aaagtacgcc gatcagctgt tggaggcgct gcgggggctg
      121 tacgcctatg aaaccagtct gaaaaaagaa gcggacggcc agtgcgacac gctggacggt
      181 cagctgaaag ccctgaccgc cgccatcgcc gacgggcgca atcggaacca gcgctttgac
      241 gagctggaac agtggaaaca ggagttggcc ggtcttctgc aaaaacaaag cgccgtagag
      301 gagcgaaagg ccgcgctggc ggcggctcgc agtgctcaac tgctggggcc gaccgatcag
      361 ctgcgccagc ggaaccggca aagtctggac gcggaaaaga cgcaggccgc ccggctgacg
      421 caggaaaaac aggcgcagga agccagcctt cccgcgctgg aacaggcggc gaagcaggcc
      481 cgggagcagc ttccgctggc ggaggaaaac gtccggctgg tcaaacgtct ggaaagctgc
      541 ctgaaatttc tggaaaagcg gcagaaggac acggcggaga tggaacggct ggaccgtttg
      601 cagacaaagg ccttggagga cagcaacagg gccgagcagg aatatctggc cgtgcggcag
      661 gcttactacc gcagccagag cggattgttg gcccgggaac tccgggaggg cgagccctgc
      721 cccgtatgcg gcgcgaccca ccatcccgcc cccgccgtcc tgcaggaagg cggggcgaca
      781 caggagcagt ttcaggcggc ggacgatcac cgcaagcggc tggaagaaca gctgcataaa
      841 atttccagcc agaccgccgc agctcgggct gcattggaga ccctgcaaaa gaccctgcag
      901 gaggaaggaa tcggcgacga ggacacggaa cagagcgtcc gtcagcgaat gaaagaagcg
      961 gacgcagccg cccggaaaat tcgggaagca gcggaacgga tggaaaccca gcggagtcag
     1021 tgccagagcc gtctggaaaa ggccgccgtc ctgctggaag cctgcctgaa aaagacaggc
     1081 gagctggaaa aggaagccga ccggctggac agagagtttg ccgcagggct ggcgcagcag
     1141 ggcttcgagg acgaaacggc ttaccgcgcc gcctgcctgc ccgcggaaga aacggaacgg
     1201 ctggacgggg ccattcgccg ctatgaagaa aaaaagaaat cgctgaacga ccgggttcag
     1261 gagcgggaaa agcagctgaa ggacagcgca cgggccgata tcgaagccct gctgcagcag
     1321 cagaaggcca tgacggagca gtaccggcag gcgcaggagc gaagccgggg ctgcgaccgg
     1381 cggctgacgc tgcatcagga ggcgggaaag gccatccgcc gggcggcgga gcagaagaaa
     1441 gcccacgccg aggaatgggc ggtgctggac gacctgtacc gggccatgag cgggcagctg
     1501 agtcagaagg tcaagatcac cttcgagacc tacgtgcagc agtattattt caagcaggtg
     1561 gtagcggcgg ccaacaagcg gctgacccag atgaccgacg gcatgtttgt gctgcggctg
     1621 aaggaagaag cgaaggatcg ccgcagccag tccgggctgg atctggacgt gctggaccgg
     1681 gccacgggcc agtggcggga cgtgtccacc ctgtccggcg gcgagagctt tctggcttcc
     1741 ctgtcgctgg cgctgggcct gtccgacgtg gtgcagagcg agagcggcgg tgtgcggctg
     1801 gaagccatgt tcatcgacga gggcttcggc acgctggacg aaaacgccct gcgcaacgcc
     1861 atggcgctgc tgtcctcgct ggcggacggc gatcggatga tcggcgtgat ctcccatatg
     1921 cccgagcttc gggagcgcat cgacaagaag atcgtggtgc ataagaaact gaccggttcg
     1981 gagatcgagc tggaatattg agcaaaatcg gaaaggaaaa ccaacatgaa atacggattg
     2041 cagatgtatt ccgtgcggga cgcggcggaa aaagacttgc tgggcacgct ggaaaaggta
     2101 gcggcgttgg ggtatcacta cgcggagttc gccggtttct tcggctattc ggcggaagcc
     2161 gtgcgggaca agctgctgga atgcggcatg gaggtctctt ccacccacac ggggatgcag
     2221 gaactggcgg atgatgtgat cggagagacc attcgatatc ataaaacgct gggcaatcgg
     2281 ctgatcgttc ttccgtgggc cgatctggac aatcagcaaa agctggacga ttttgtcgcc
     2341 caaacagaaa aggtgcaggc acgactggcg gacgagggca ttcggctggg ctatcacaat
     2401 nnnnnnnnnn ctcctgcatt tgggagcagc tggaagaacg cacccggctg gacttggaga
     2461 tcgacaccta ctgggccttc gtgggcgggc gcgatccgct ggaaatgatg gagcggctga
     2521 aggaacgcgt ccgcatcatc cacatcaagg acggactggt ggacggcagc ggaaggccgc
     2581 tgggcatggg ggaagcccct gcggcggcgg tctaccgtaa ggccgtggag ctgggcgacc
     2641 ggctgatcgt ggagtcggag acctgccagc cctcgggact ggaagaagcc cgcatctgca
     2701 tggatttcct gaaagcgcag gaataaactg cgcagaacgc cgaaaggccg cgccggaaaa
     2761 cggctgcggc ctttttcgtt gagaaaaata ggggaagggc ccggcacgat tgcgggaaaa
     2821 ggtttatcgc agccgcttca gcacggcggc gggattttcg cccttttcgg cgcagaaggc
     2881 tttgtagaag ttggcgtagc tgcggaaccc gcattccagc gaaatggcct ccagcgaaag
     2941 gtcggagtag cgcatcagcc gctgagcttg ttccagacga agaagcgtca ggcagcggtg
     3001 gggcgtggtc tgataccggc ggcggaaaat gcggattaga tagctctcgc tgacgtgcag
     3061 ctcggcggcg atttgggcga gagacgcgcc gtccttcaag tggtactgca actgctgcag
     3121 ggcgctttcc gccagccgtt ccgcaggggt ttgagggcgg ctgtggcaca aaagagtgag
     3181 aagctggtaa aacaggctgg tgcgctccaa aagcggcgcg cccagctgat cggcctcggc
     3241 gagactgcgg gtgagggcct gcgcctccgc ag
//
LOCUS       DAWAEA010000139         3253 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4484_length_3253_cov_1.839275, whole genome shotgun sequence.
ACCESSION   DAWAEA010000139 DAWAEA010000000
VERSION     DAWAEA010000139.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4484_length_3253_cov_1.839275"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    269..473
                     /estimated_length=205
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          606 a          932 c          852 g          658 t
ORIGIN      
        1 gccgtggact ctgcagggga tcccgcaggc ccttaaaagc gccatgaaca gcgttccctt
       61 cgtgttgcac tgcccgtacc cgtctgccaa aactctggag gcgggaatgc tgtcgtccac
      121 attatacccg aactgtatct cgtcccgtac aaaaagatag atggatttca gacgctccaa
      181 ttcttccatt tccttccagc cttttttctc tatcaactgc cggatttggg cgtttgaata
      241 gtccagcata ggggtttcca tcaaatagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctcctga
      481 gccttcacct tccgatattt tggaaattcc tcgccgatcc tgttcagcgc ctcctgatcg
      541 tccagctttt caaagtcctg gctttcgatc agtttcccgt ggatgaaaga aaaatcgtca
      601 ttcaagggaa agaccatcag cgcgtcagga gaattgcccc aagcgtccgt aatgccgaac
      661 tccgcccctc tgcggaagcc gaagcgggga taataatacg gttcccccat aatgataatg
      721 cccgcagcgc cgaatgaccc ggcaaggcca agagtttccc gcaccagtgc gccgccgatg
      781 tcgttgccct ccatcatcgg ctccaccgcc agcggcccga acgtgacgac ctcgcgccgg
      841 gtatcgccgt ccacgatcca tgctcttgtg tagtagatcg ccccgacgat ctgtccgttc
      901 cactccgcga cccggctcag ttccggcaca taatctcggg agtctcggat gatccgcgtc
      961 agcagatgct ccgtgcatcc cggccaatac ttgttccaaa agctgcgcat gaccatctgt
     1021 tccgtctggt gatagtcggc aggcgtttcc tgtctgataa tcagatcctt cagcattttc
     1081 cggctccttt tctggatgaa agcggcgttt ttccgaatgc agacttgcgt ccccgtctag
     1141 ggaatatcct atcatacgtt ccatagaatg gcaatgacgc aaaacggccg cagcatattt
     1201 ccatgccgcg gccgtttgaa tttctatgcg cccttcgtct gaaaaaggcg tttggttttt
     1261 acttgcggta cagcacttcg ctgcgcttgg gaccggtgga gagcatccgc accggcactt
     1321 cgatttcctt ctcgataaat tccacgtact tgcggcagtt ctcgggcagc ttgtcccatt
     1381 cggtaatacc gcgcacgtcg ctcttccagc cgggcagcac ggtgtacacc ggcttggccc
     1441 gcatcaggga aggcgtggag gggaagtccc gcaccagctt gccgtcgatc tcgtaggcgg
     1501 tgcagaccgg gatctcatcc agatagccca gcacgtccag attggtcatg gcgcactcgg
     1561 tagcgccctg cgccatgcag ccgtaacggg tagctaccgc atcgaaccag cccacccgac
     1621 gaggacgggc ggtggtcgcg ccgtattcgc ccgcgtcgcc gccgcggtcg cgcagttcct
     1681 tagcttcatc gccgaacagt tcggtagtga aagggccagc gcccacgcag ctggaatagg
     1741 ccttggtcac gcagacgatg tcgctgtagg cggtcggagg aacgcccgcg cccacggtgc
     1801 cgaaaccggc cagcggagaa gaggacgtgg tatagggata gatgccgtga tcgggatccc
     1861 tcagcgtacc cagctggcct tccagcagga tggttttgcc ttccttcagg gcctggtgca
     1921 ggaagctggt ggtgtcgatc accatgggcc gaatctgctc cgcctgcttc ttcaggtgag
     1981 cgatcagggc ctccaaatcg atctcgggct tgtgatacag gttgcgcagg gtggcgttct
     2041 tctgctccac cgcgctggaa agccgggcgc gcagcatgtc ctcgtcatac agctggcaga
     2101 cctgcacgcc gatcttcagg tatttatcgc cgtagaaggg agcaatgccg ctcttggtgg
     2161 agccgaagcc cgccttgccc agccgttctt cctcatactt gtcaaaggcc acatgatagg
     2221 gcagcagtac ctgtgcccgg tcgctgatgt acagcttggg catgggcacg ccgcggctga
     2281 ccagactgtt cagttcctcc acgaaccgct ccacgtccag cgccacgccg gggccgatca
     2341 cattgacgat gttgctgtgg aacacgcccg agggcagcag gagcagcgca aatttaccgt
     2401 aatcgttgat aatggtatgg ccggcgttgg cgccgccctg aaaacgaacc acgatgtcgc
     2461 tgttctccgc catcacgtcc gtgatctttc ccttgccttc gtctccccag ttggcgccta
     2521 cgatgcttcg aaccataact gatctctcct tcactcgctt ctgcggccgg gacaaaaata
     2581 agcataggcc attccccttt atactatatt taagcaagaa atgtcaagcg gacagaggag
     2641 gtcttttgca tgaaggagaa ccgtcttttt ccccaaaaat gccgaagggc gctgctggcg
     2701 ctggccgccg ccgggcttct gagcttttcc ggcgcgctgg cggcggacta tgccgacggc
     2761 gtttatcagg atctggcccc cggctacaac gacgacgtga tcgtctccgt cacggtgaag
     2821 gacggcagga tcaccgagtt cgacgcgaaa aaccgcaacg gcaacgaatc cgaatacttc
     2881 ggaaaagcct tggagggcat ccgtcaggcc gttttggaaa agcagggtct ggatggcgtg
     2941 gatgcggtca gcggggccac cggttcttcg gacagtatgc tgaccgccat gaaaggcatt
     3001 ctggagcagc ttcgcgcagg cggcggagag ggcagcgtca gcagcgaaaa cacgccgtct
     3061 gcccagccct cccccacggt cgatcccgcc acggcgcagg tcttttccgg gcttggcagc
     3121 acggccaatt tccgcaacgg ccccggaaag gacgcggagg gcacgccggt ctattccttc
     3181 aacatcgcca tggcgaacgc gctctttgac cgggagggca aaattctgaa cgtatttgtg
     3241 gacgtgtatg aag
//
LOCUS       DAWAEA010000140         3238 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4514_length_3238_cov_1.716305, whole genome shotgun sequence.
ACCESSION   DAWAEA010000140 DAWAEA010000000
VERSION     DAWAEA010000140.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4514_length_3238_cov_1.716305"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1244..1253
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2452..2461
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          658 a          969 c          916 g          675 t
ORIGIN      
        1 caccagcaca ccggcgttct tgatcttttc cagtacgccc tccgcagagg cggacacggc
       61 gcaaatgatc agacacaggg ccaacgcgat cgcgaaaatt ttcttcatca tggtttcgtc
      121 cctccaaaat cgttagaatg tttattcacc ttacgtgaat gattatacat ccgttttgca
      181 tgttgtcaag aggaaaaatg aaaaaagttt cacttttttt gcgtggagtt tttagccggt
      241 cagaagcagg cgctcaagtc cttcaggcgc tccatggcct cctgcagctt ttcatccgaa
      301 acggtgcagg caatgcggaa atgtccctgc cctgccttgc cgaatacgct tccgggcacg
      361 gccagaatat gggccttttc caaaaggaac tgacagaatt ggccgtcggt catgccggtc
      421 ttggcaatgc cggggaacag ataaaacgtg ccccggggcc gctgcagggt catgtagggc
      481 agcttttcta tgcattcgga tgcataaaat actcgatccc gatatttgca tatgtactcc
      541 tcggcgatct ggtgccgcag ggccagcgcc cggatggccg cccgctggga aatggaggga
      601 gcagtgtacg tcagcgcatt gctgataaac tgaaacaccg aaatcagctc cgccggggcg
      661 atgaccacgc cgacccgcca gccggtcatc aggaaattct tggagaagct gttcagcgtc
      721 accgtgcgct ccgccatacc gggcagggtg cgcatgggaa tgaactcgcc ctcgtacaga
      781 taccgggtgt agatttcatc cgccagcacc agcaggtcga actccttggc tatgccggcc
      841 agcatttcca gcgtgtcccg gtcgtaggcc gcgccagtgg gattgcaggg ggtgttgata
      901 ataatggccc gggtacgggg cgtaatggcc cggcgcagct cctgctcatc gggtcggtag
      961 gcgttttcct cataggtggg cacttccacc accacgccgc ccgccagttc gatctgctgg
     1021 gcgtagctgg caaagtacgg ggccagcacg atcacttcat cgccggggtt cacgatgcca
     1081 agcagcgcct gcgccatgcc gatgctgctg gaggtggtca ccagcacttc ctcccgggga
     1141 atgtcctgcc cgaagtcctc tttccacgcc tgaatgaccg cgtccaccag ctccgggtct
     1201 cccttggggt cgccgtagtg ggtgtagccg ttcagcgcgt cggnnnnnnn nnnccgcata
     1261 acgggccaac gcctcggtgt caaggctcag ccctgccggg cgggtttcaa aacgtttggc
     1321 aacaaaggaa cgcatctttt cctctcccct tttacttctt ttctttcacg taaggcacca
     1381 cggtgctgcc gttgggaata acatacaggc tcttgccctc cagatgagcg gccttcatca
     1441 gctcgtccac gtcggagaag gccttcagcc ccatgggggc cactgtctcc tgcggcacag
     1501 aggtcagcat gagaatgttg tagcggtgcg cctgttcgca gttcagcagg aagatatagc
     1561 cgggcacggt gaaatgctcc cgcaggcgct tttccatcgt gccgcccagc agatcgtcga
     1621 tccatccgaa atactcggcg gggccgccgc cgtcccgggc ctcgatgagc aaaatcagcg
     1681 tgccgccggg cttcaggccg gtttccacgt tgtcgatggt cttggtgccc tgatacaggg
     1741 acatatcctt ggggaaaccg ccgcagctgg cgatgatcgc gtcggccttc tccggcactt
     1801 tcacccggta gatgcggtcc accgcctcgc aggccttctg ccatgaggtc aggtagtgac
     1861 cggcataaat ttccgccagc tgcatttccg cgttcatcac cagattcacc atatacagct
     1921 ggggaaccat ggccgcggcc tcgcacatat cgtcattgag gggattgtcc ttcaccttgc
     1981 cgttgccgat gcggggattg ctcatcaggc aggccgggtc cagcgaatag gcatggttgt
     2041 ggcagatggt ctccatgccg ctgatgccgg gcaggatgct cttgcgtccg ccgccgaagc
     2101 ccgccatgac gtggtgggtg caggcgccca gacagatcac ccggtcggcg tgagcggctt
     2161 cggcgttgat ccacaccggg gtgtcgtggg ccgtggtgcc cagatacacc agatccttcg
     2221 ccagacagtc gtggttcacc acccgcaccc ggtcgtatac gccgtccgtc accagcgtgc
     2281 gcagctcttt ttcatcgccg ccgtcgtgag tgccgttggc cacaatgatg gtcacgtgat
     2341 cctccgggat gccgcactcg tcccgcagat aatccaccag cggcgggatc accttaccct
     2401 gccgcatcca gaaccgggac atatcgctga tgaccagcgc caccccttcg cnnnnnnnnn
     2461 ntgggatttt ccagcgcggc ccgcagggta tcgggaatgg aggcggctgc gggcacatga
     2521 ttcccgtgca gctcccccaa cacctgacct tcgtccagag gaatgacgac ctccccgtcg
     2581 ccgtagcgga accggtactc tttcatgccc atgcgcctcc atgaatccat tcatatctat
     2641 gcatcgaaat gcatatgcga aattttatca cgggcggcgg gttttgtcga ggaaaaacag
     2701 gaagagtaca attttcattt tctttcggga aaatccccca caatgtccct ttacacgccc
     2761 gcttcagctg gattataatg aaagtacccg tcagagcggg tgatcggctt tcatcaacta
     2821 aaattggata tggaggatat gcctatggat cgcaaactgc atatcggtat tatcggctgc
     2881 ggcggcattg ccaacggcaa gcatatgccc tcgctgaaaa agatcaacac ggtggagatg
     2941 gtcgctttct gcgacattgt gagggaacgg gcggaaaagg ccgccaagga atacggcacc
     3001 ccggacgccc gggtgtatac cgactacaaa gagctgctga aggaagatgg catcgacgtg
     3061 gtgcatgtgc tgacccccaa caagcagcat tccttcatca cggtggacgc gctggaagcg
     3121 ggcaaacacg tcatgtgcga aaagcccatg gccatcaact ccgccgaggc caagaagatg
     3181 ctggacgcgg ccaagcgcac cggcaagaag ctgaccatcg gctatcagag ccgcttcc
//
LOCUS       DAWAEA010000141         3234 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4523_length_3234_cov_2.727273, whole genome shotgun sequence.
ACCESSION   DAWAEA010000141 DAWAEA010000000
VERSION     DAWAEA010000141.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4523_length_3234_cov_2.727273"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    898..945
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          685 a          877 c          975 g          649 t
ORIGIN      
        1 gcagggaacg ttgctgctgt atctacttct gcagctgaaa aagtcaggtg tgcgctgtat
       61 accggctctg ggagcggcac tggcggtgtt acaggcgtgg ctgcttccgc tgtgtctgca
      121 gtattcgacg gcctggctga cagcgttggc cgccatgtgt ctattggtgc ggtttcagag
      181 ccgctttgaa aaagacgggg ttgttctgtt tttcctcggc acgggcatgg tgacatctta
      241 tgtggatttt ctgacctatc cactgattgc ctttggcctg ccctgcgtcc tctggctgat
      301 gctggaaaag gatcgtcaga agaatgcact tttgctgctg gtacagttgg cgctggcttg
      361 gggaatcggc tatgcaggaa tgtgggctgg caaatggctg ctcgttcttc tttttggccg
      421 tgccggggaa ggcagcatga ttcttgcggc catggaagaa agaggactaa gtgccgctgt
      481 cagcccgtgg agcgcaattc tgcggaatgg gagcgtattt tggcggaaac cctataagct
      541 ggcgctgttt tgcgtgacaa tttggatgat agtccggctg attcggctgg ggaaaaatcg
      601 caggaaggtt tcgtggaatc tggcaatggc tctgctgata acggcggcca tgccgctggc
      661 atggtatgcg gtgatgaaca atcacagcca tgtccactat ttctttacct atcggaattt
      721 ggcgataagc gtgtttgcct tgggatgctt ggcttctttg cttctggaag ataaaagaaa
      781 agcaaatgaa aaaggcttgc cagtatagca ggccttttct cttgcgattg cttttttacc
      841 caaaacacgc tatgatataa gatgatgaaa tttacttgct tggagggaac aacagttnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncggct gtcccatatt
      961 cgcaattttt gcatcattgc ccatatcgac cacggcaaga gcacgctggc ggaccgcctg
     1021 ctggaaatga cgggcgtgtt tgagaagcgg cagatgacgg agcagatttt ggactccatg
     1081 gaactggaac gggagcgcgg catcaccatc aagtcgaagg ccgtgcgcat ggagtatcaa
     1141 gccaaggacg gagagactta cgtcctcaac ctgatcgaca cccccggcca tgtggacttc
     1201 agctatgagg tcagccgggc actggcggcc tgcgaaggcg cgctgctggt ggtggacgcg
     1261 acgcagggca tcgaggcgca gacgctggcc aatgtgtata tggcgctgga acacgatctg
     1321 gagaccgtgc cggtcatcaa caagatcgac ctgcccagcg cccagccgga ggaggtcaag
     1381 cacgagatcg aggaagtgat cggcctggac acctccgacg cgccgctgat ctccgccaag
     1441 acgggcgaaa actgcggcga cgttctggaa gccatcgtca ggcacatccc cgcaccccag
     1501 ggcgacgcgg aagcgccgct gcaggcgctg attttcgacg cttattatga caactaccgt
     1561 ggcgcaatct gcttcgttcg ggtgaagcag ggcacggtga agcccggaat gcggatgcgg
     1621 ctgatgcaga ccggcagaga ggtcgacatc gtggaggtgg gcaccttcca cccgggctac
     1681 gagtcctgcg acgagctgta tcccggcgac gtgggctaca tcgccgcctc catcaaggat
     1741 gtggccgaca cccgggtggg cgacaccatc actgacgcga accgcccggc ggacgcgccc
     1801 ctgcctggct accgcaaggt cacgcccatg gtgttctgcg gcatctatcc cgccgacggc
     1861 gcggattacg acaacctgaa ggacgcgctg gaaaagctgc gcatgaacga cgcgtccctg
     1921 tccttcgacc cggagacttc cgtggctttg ggctacggct tccgctgcgg attcttgggc
     1981 cttctgcaca tggaggtcat tcaacagcgg ctggaacggg aatttgattt ggatctggtc
     2041 actaccgcgc ccagcgttat ttaccatgta tacaaaacgg acggttccat ggtggagatc
     2101 gacaatccct ccaacttgcc gcccatcggc gagatcgagc acatggagga accctacgtc
     2161 accgccacca tctatacgcc gcaggagttt gtgggcgcga tgatggacat tgccgtggac
     2221 aagcggggca ttttcatcga catgcagtac gagggcggcc gcgtcaagct gatctacgag
     2281 attcccctga acgagatcat ctacgagttc ttcgatcagg tgaagagccg cagccggggc
     2341 tacgccagct tcgactacga actgcacggc taccgggaga gcgatctggt caagctggac
     2401 attctcctca acggcgaaat ctgcgacgcg ttttccatca tcgttcaccg ggacaaggcc
     2461 tacgcccggg gacggagcat cgctgaaaag ctgaaggacg tgattccgcg gcaactgttc
     2521 gaaattccca ttcaggcggc catcggcggc aaggtcatcg cccgcgaaac cgtccgggcc
     2581 atgcgcaagg acgttctggc caagtgctac ggcggcgaca tcacccgcaa gaagaagcta
     2641 ttggagaagc aaaaggaagg caagaagcgc atgcgccagt tcggcaccgt cgaagtcccc
     2701 agcgaagcct tcctcgccgt cctcaaaatg aacgacgact agcagggact gagcccctgc
     2761 accccacacc gcgccctgcg ggcgcggggc cgctcggggg acatgccagt tggccggggt
     2821 tttaccgccg ctcggcgaca ggcctcgcgg cttgccggtc tggtgcggct ccctgctgct
     2881 tgaacagtaa agaccgccat tcggcgacgg gcctcatggc tcgctaaaaa ggccggcgtg
     2941 gatttgaaat cccgctggcc ttttgtgttg caaaaatttc caagctacat tgaagcatcc
     3001 caccccgcaa ggggtaagcc tgccccgcgc caactgcggg ggagcagagc ggcgataggc
     3061 agcggaggga aaaagccttg ccctttttga aagaggaaaa gagtgagcac gagcgcgcac
     3121 gatagtaggt aataactatc caacaatgct gcgcgcgaca ttgcgaactc cctctttcaa
     3181 aaatgggcaa taaagggctt tttcccggcg ggtgagcgca gcgaactgtc ccga
//
LOCUS       DAWAEA010000142         3230 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4529_length_3230_cov_1.636850, whole genome shotgun sequence.
ACCESSION   DAWAEA010000142 DAWAEA010000000
VERSION     DAWAEA010000142.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4529_length_3230_cov_1.636850"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1737..1746
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2830..3013
                     /estimated_length=184
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          577 a          934 c          935 g          590 t
ORIGIN      
        1 agtggccagt tccaagaaat accgcatttt cggccacggc ggatgcggcc ccactgccgc
       61 cgccatgatt ttggccaatc tactgacctc ggaagaactg acccggctgg atcagttcac
      121 cgaaaatggc gagggcttct atttctgccc ctgctcggtg aaccagtttc actgccagag
      181 ctatcacgtg aaatatcacc cgaccaagcc ggaggaattt cagcgctatt tgcccattct
      241 catcggcaat atggccaccg gcaacaactg ctggggactc aaggcccgga gcaccaactg
      301 gggcagcagc ctgaattatc tggaaaagct ggcggacggc ttcggccttc agatcgagaa
      361 aaataccgat cttcacgaga ctgtcgctgc cctgcaggat cacagcaaac agcggctggc
      421 gctgacctgc gccacgtggg gcagcccctt caccctgtcc agccatttcg aggtcatgtg
      481 ctacgccgac gacgaatggg tctactttct cgatcctctg cgggacaaca actatgccaa
      541 gaaccgcaca ggctcgctga tcgacgttgt ggctcccggt gtggtgcgga tcccgcttga
      601 caaagtgtac gactgcaata tttcttccta ttatatcgtc gaacggccct gaggcgttct
      661 cgccggggca ggaggaaaaa atgaaaggga actccatcca cgggctgacc acggaggaag
      721 ccgccaagct ccgggaagcc gggcgcggca atcagcagcc caaaagcaaa gacggcggcg
      781 taggcgagat cctgcgccgc aatgtgttga ccctgtttaa tctgctcaac gtcattttgg
      841 ccctgctgct gcttggtacc ggcagctacc gcaacatgct cttcatgggc gtggtcgtct
      901 ccaacgctct tatcggcacc attcaggagc ttcgggccaa gcgcacccat gaccggctgc
      961 agcttttgag ccgcagtcag gtctccgtcc tccgagacgg ccgggaagtc cctctttccc
     1021 cggacgatct ggtagagggc gacgtggtgc tgctgcgccg gggcgatcaa attcccgcag
     1081 acgggcaggt cctggagggc gaagcgctgg cggatgaatc catcctgacc ggcgaaagcg
     1141 aaaagattcc caaaaagccg ggcgataccg tctattccgg cagttatctg gtggaaggac
     1201 ggctgaccct gcgcctgacc gccgtgggag cccagagtta tatgggccgt ctgcaggccg
     1261 cggcccggca ggtgaagcgg cctaaaagcg agttaatggc ggacatgaac cgcatcatcc
     1321 gctgggtgtc cgccctgatc gtcccggtgg gtttgctgct gtactggaag caaaccgccg
     1381 tattgggact ggctcactcg gaggcggtgg tcaagaccgt cgcgtccatt atcggcatga
     1441 tccccgaagg gttgatgctg ctgacctccg tgtcgctggc ggcaggtgtg gtaacgctgg
     1501 gccagaaaaa aacgctggtc aacgagctat acggcatcga atcactggcc cgggtagacg
     1561 tgatctgtgt ggacaaaacc ggcaccctga ccagcggcaa aatgacgttg gaacagacga
     1621 tccccgcgcc ggatgtgtcc aaggaacggc tgcaggatat actggcttcc ttcctgtgcg
     1681 gcatgggcgc ggacggttct accgccgcgg cgctgacgga ggcctgtccg ccccggnnnn
     1741 nnnnnngcct ttttccagcc agcggaaatg gtcgctggcg gtattggaag gcgtgggaag
     1801 cgtgatcctc ggtgcgccgg aacggctgct ttccggcgcg gagctgaaaa aggcgcagga
     1861 gctggcagcg caagggcttc gggtgctggc cctgatggag agccccgaac cgccgtccgt
     1921 tcaggccgat gacgttcaac tgcccgccca gcgaaagctg ttggcgctgc tctgcctgtg
     1981 cgacgggctg cggccgcagg ttcgccagac cgtcgagtat ttcggccggg agggcgtgca
     2041 gatcaaggtg atctccggcg acagcccgct gaccgtttcc agtgtggccc agcgggcggg
     2101 cattcccggc gcggaaaagc agatcgacct ttcccaactg gagggagaga aggactacac
     2161 ggccctgagc cgggaataca ccgtattcgg acgggtttcc cccaacgaca agcgggagct
     2221 catccgcgcc ctgcagcggg acggccatgg cgtggccatg gtgggcgacg gcgtgaacga
     2281 tattcccgcc ttgaaaacgg cggactgctc gctggccatg gccgggggca gcgacgcggc
     2341 ctgccgggtg gcccagatga ccctgctcag cggtggtttt gacgcaatgc cccagattct
     2401 tctggaaggg cggcgggtca tcaacaacat cacccgggct gcgtccctct ttctggtgaa
     2461 gaacctgtac agtatgctgc tttcactggc actgctgttt ctgccctttc cttatccctt
     2521 tgcgcctatc cagctgaccc ttatcagcac tctgaccatc ggcgcgccgt ccttcattct
     2581 ggccctgcag cccagcaggg aaaagacgca gggacgtttt ctggaaaata tcctgctgcg
     2641 tgctctgccg ggcggcatct gcacggcggt gctgctgctg gccgccctgt gtctgcggga
     2701 gccgctgggg cttgccgccg cgcaggaaag cactctctgc accgtggtgg ccgctgtctc
     2761 cgggctggtg acgctggcgc tgacctgtct gcccttccag tggctgcggg cctgtgtggt
     2821 ggcgctgatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnaagcggc tggaacgggc tttccgccga aagaaaaagg cccccgggaa
     3061 accttctttc ccggagaccc ctgcagcccg ctgacgcttc agccctgcga catttgcggc
     3121 gcttctctgg tgaagccgct cttccccgtc cacggattcc agacgtgata ccatccatct
     3181 tccaaaatgc ccattccata ggtttcatac caactgggcc attccatcac
//
LOCUS       DAWAEA010000143         3206 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4578_length_3206_cov_1.717233, whole genome shotgun sequence.
ACCESSION   DAWAEA010000143 DAWAEA010000000
VERSION     DAWAEA010000143.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4578_length_3206_cov_1.717233"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1301..1310
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2263..2272
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          643 a          996 c          968 g          579 t
ORIGIN      
        1 catcagaccg tgggcctgca cagtgagcgg gacgcgaagc tgaacgtgct tcgttttggc
       61 ttcatccgct accccatgat cgacgcgggc gaatgcttca ccatggcccc catcgactgc
      121 tatccccatc tgggcgactg gcacgcgggc gcgaagctgt accgggcctt catggaggaa
      181 aaaggcggct ttgtgccgcc cgtcaatccc gagtggagcc gggacatgac cggctggctg
      241 cggctgatct tcaagcagca ccactgcgag ctgaactgga gctacgccga cattcccacc
      301 atgtatgacg agctggaagc ctgcgggctc aagaccctgt atctgctggg ctgggaagcg
      361 ggcggctttg cccggctctg gcccgatgaa cgcatgggcg gcgaaaaact gctgcgggag
      421 ggcatcgagt atgtccaccg aaagggcggc aaggtgctga tgttcctcag ctatgccctg
      481 atcgaccatc acagcgactt ctatcagaag gaaggcggcg acaaggccac catccgctct
      541 atctggggcg aggatattcc ctttgccgaa acctactgcg gcgagggcac ctatcggaaa
      601 atcggcaatc cggccatgcc catgtatctg gcctgccccg gcgcgccgat ctggcaggaa
      661 aagatgaagg aatccgccaa aacctgtctg gagctggggg cggacggcgt gctgtacgac
      721 atcggctcct ggtatcccta cttctgctat gccgagggcc acacccacgc caagccgagc
      781 cattctcacg aaagcaaggc ggccaactac accggtctgc gggaatacgt caagaccttt
      841 gggcaggaca agatcatcct catggagcac aacacggaca ttttcggcca gtccatggat
      901 atttcccaca gcgccaacac ccagcccccg gtgcgtctgc gctccccgga aacagcccag
      961 cacgccacgg acgtgcgcga cgatctgttc atgccggagc tgtaccgcta cactttcccg
     1021 gaattggtag ccaccaaccg ggaatgcggc gaggacgagg aaagctacaa ggccatgtcc
     1081 gggttcagtt tcctgtatgg tctgcgcttt gacatgacca tctaccgctg ctgcggctct
     1141 ctgcgggata ttccccggta cgccgcctat ctgaaggagc tgtccgccct gtacgagcag
     1201 tacggcgact atctgcttcg gggccgcttt gtggacagcg acggctttat ttcggacaac
     1261 gaaaacgtgc tggtcaaggg ttgggaaggc tgccgcggcg nnnnnnnnnn ccctccgcag
     1321 ccgccaaacc ggccgcgccg tgaccgtcgt cgtccccgcc gagcgcgccg ccgccgccgt
     1381 gatcgacgaa gcgtaaaacg cataaagaga cgttggggcc tatgcggccc caaaccccgc
     1441 agttaagggc tcatgccctt aacaatccca ccattgaccg cgcaatgcgc ggtcaaaaag
     1501 gggttcaggg caaagccctg acgaggggtt taggggcgag cagcccccaa cgtctcttct
     1561 tattcgtccg tgagcggcag ggtgcggcaa aaggcgcgga aggcctgcca ttcctcccgg
     1621 gcggcacagc ctgcccagag catcaggttc ttttcccggt ggcgcagcgc cgaggaagat
     1681 aaaaatagct tctgctgaag cacctccggc ttttcgccct gaagtaggcc gaaaaatagc
     1741 tgccggtcgg tcggatcgca gcgatcaagc agcgtttcca gcgccatcag ccgcagggtt
     1801 tcgctgtcgc cgtaaaagtc caccgcttcc cgaagcggcg tggaaagccc ttcccccgca
     1861 ggcctcagcg gccggttttc cgtggattcc cgaaccgcta cgcggcagtg tacctgcacc
     1921 gccacgctgg ctgtcggctc ctgccgggcc agaaaggcgt acaggctcac cgcccgccgc
     1981 cccagctcgt cgtggttcag tgacgcgctg gtgatcgccg gctgcacagc ccgggccagc
     2041 gaggaatcgc catagctgag caaatataaa tcctccggga cggcaacgcc gtcctttttc
     2101 agctgtttca aaagcgccgc cgccgcaatg tcgttggcgc agatcacgga atcaaaccgg
     2161 cggatatggg gcgcgaagtc cgcatagcac tccgtaaggg aaccgcggtt ccagaacacg
     2221 ttcggctccg ccgccccggg aaagcagcgg ctccacgcgg cannnnnnnn nngtctcctg
     2281 cttggtcagg tctgccgctg aattggggtt cagggcaaag aaggccgtct tttcccggcc
     2341 ccagtcctga aggtatttca gcagcgtccg ggtcgcttcc gcgtggttca tggataccgt
     2401 tccctctgcc ggagccccct gcgagggatt gacgctgatc agcacgcagg aaatgcccag
     2461 cgcgcccagc cgcgcctgcg ccgccgggac ccagctcatg gaggtgccca tcagcatcac
     2521 cagacggcgc tcgcggtcga agagcccgtc ccagtcgatc tgctcctcct gcgccgcgtc
     2581 cagcagcacc agctcgtatt tttttcgggc agcttcccgg gcgatgccct ccagcgtctg
     2641 ccgggcccag agggagtttc ggtaatccgg ctccaccaga acgggaatgc gcatggagac
     2701 ggcctccttt gacgaacttt ttcgttatga tagcacatgg gagaaacgaa ggtcaataaa
     2761 ggctttcgcg caccgtttct ttgcgttgct tgccgcaacg ggccattttt cgtacaatga
     2821 ggagtgccgg caagaaaaga aagaagggat gcccgatgct gagtgaaaat atcagggccg
     2881 cacggaaagc cagagggctt tcgcaggaag aactggccgc aaggctgaac gtggtgcgtc
     2941 agacgatctc aaaatgggaa cagggcttgt cggtgccgga tgccgacctg ctcatcggcc
     3001 tgtcggaggc attggaaatt cccgtcagcg ctttgctggg cgaaaccgtg cccccggaaa
     3061 agccggacga aatccagatt ttgtccgaaa agctggaagc gatcaatctg cagcttgccc
     3121 ggagaaaaat cgcccgaaga aagcttctgc gcggggcgct ggcggcattg tgcctgatcg
     3181 ttctggcagg gttggcgggg ctgtac
//
LOCUS       DAWAEA010000144         3188 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4603_length_3188_cov_2.029684, whole genome shotgun sequence.
ACCESSION   DAWAEA010000144 DAWAEA010000000
VERSION     DAWAEA010000144.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4603_length_3188_cov_2.029684"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    691..713
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1121..1160
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          557 a          957 c         1076 g          535 t
ORIGIN      
        1 ggcgtaaagt atggcgaatc ccacctttac cattactctg gaaaacggcg gcgtgatgaa
       61 gggcgagctc tacccggaga tcgctcctca gagcgtgggc aactttattt ctctggccaa
      121 cagcggcttc tatgacggat tgatcttcca ccgctgcatc cccggcttca tgatccaggg
      181 cggctgcccc gagggcaccg gcatgggcgg ccccggctgc ccaacccgct caaacacacc
      241 tatggcgtgc tgagcatggc ccgcagcatg atgaaggaca gcgccggttc ccagttcttc
      301 atcatgacca gcgacagccc ccatctggac ggccagtacg ccgccttcgg cagggtgctg
      361 gagggcatgg aggaggcgga gaagatcgtc tcccagcgca ccgaccggat ggaccgcccg
      421 ctggagcctc agcgcatcca gtccattcgc gtggagacca acggcgtgga atatcccttt
      481 acgaagctgt aagcggtttc cggcggcgga ggggcagacg ccctttcgcc gccttttctc
      541 tggaaatgaa atgctgaagg gcttcggccc ggaggggaga aagtgccatg aaatcgaatc
      601 atcccgttcc ggagctgctt tgccctgctg gaagcgaaga agcgctccat gccgccgtgg
      661 acagcggcgc ggacgcggtg tatttgggaa nnnnnnnnnn nnnnnnnnnn nnngggcgac
      721 gtgaaaaagc tgctggaccc cagcgaggta ggcggaacgc cctttcttgg catcagcaag
      781 cctgtgctga aggcccacgg ctcctctgat gcccgggcca tccgcaacgc cgtgttccgg
      841 gcggtggaat acgcccacct gcatcatgcc cgggtgcatg tgaccgtcaa cacgctggtg
      901 aaggagcagg agttttccgg cgttggggag accctgcgaa ccgtcgccca tgctggcgcg
      961 gacgcggtga tcgtgcagga cttgggcgtg gcggcgctgg tgcgggacga gtttcccacg
     1021 ctgaagctcc atgccagtac ccaaatggct ctttccaacg ccgacgacgc ggtttttgcc
     1081 cggaacttcg gcttcgaccg ggtggtgctg gctcgggaat nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn gacgaagcgg caaccggggc cggtgtgccc aaccctgccg
     1201 aatgcgtttt gaatggaacg gccgggaggc ggcgtggctt tcgctgaagg atctgtgcgc
     1261 ctaccccgtc ctgccggagc tgtgggatgc gggcgtatgc tccctcaagg tggagggacg
     1321 gctgaaaagc ccggaatatg tggccgtagt gacggaagtc taccgccggg cgctggatga
     1381 tctggcggcg gaacggtttg acccggaaga cccggccccc atggaaaagc tgcggcagat
     1441 cttcaaccgg ggcggattta cccggggcca tgcgggcggc gcggaggacg cggccctgtg
     1501 cgcgccggaa cgggtgagcc acgagggtct gccgctgggc acgatcacgt ccgtccgtgg
     1561 aaatctggca cgggtacgat tgattcgttc gctgcacgac ggcgacagtc tgcaactgcg
     1621 ccagcggcag gacgacgacc tgcgctattc cggcccggag accccggccg gtcaggaggc
     1681 agtgctgcgg cttcggcccg gtctgacggc ggcccccggc acggaggtgg cgcgtctcag
     1741 cgacgcgaag cagctggaag cggcgcgcag ccacgctccc aaacccattc ccgttcaaat
     1801 gcgcggcttc tttgacctga accgccccat gacgctgacc ctctctgatg gggaaagcgc
     1861 ggtgacggtg gagggacacg ccctttgcgc tggaaacacc ggacgatctg gcgcttgagc
     1921 tggcggacgg cctgtttttg ccggtcagcg ccctgaacgc cctgcgccgg gagggaatcg
     1981 cggctttgac cgctgcgaga atcgaagcct tctggcagat gggacaggaa acgcataccc
     2041 gggccagaaa accgtggggg aagactctgt ccccgactgg cggcgagccg ttgaatgcgg
     2101 attcgctgac cgtctgcttt tccgacccgg caatggcgga cgagctgctg caatgcggcg
     2161 cgaaccggct ctggttccag ccccgcagct atcgtctgtc agaactggaa caggatttga
     2221 accgactgcc tgacggctgc tggctggtgc tgccggtaca atgcaccgaa cccgagctgc
     2281 acggcattcg ccgggcggcg gagacggcga agctgggcgg tatagtggcc cagagcatgg
     2341 ggcagatccg ctttgccgac cgttggcccg tggttttcgg cgacgcgctg ccggtgacga
     2401 accggcgggc gctggaagcc ctgaggaagc tgcccgtcag cggcttcacc ctgtggccgg
     2461 aatggacgga gcaggaacag cgggagctgc tgcccctgcc gctgcccgcc ctgtgcccgg
     2521 tctatggccg ggagcggctg atgattctga accactgccc ggcccgggtg agggagggcc
     2581 tttcccacgg gcgggaggcc tgccggttct gccgcacccc cgatcaggcc tgcgggcaga
     2641 tggagccggt gctgcgggat gggcggggct atgggttccc cctgcggcga acggagttct
     2701 cggagggctg tcaggtgaat gtctacaacg ccctgcccac cgatcttcgg gccttcgacg
     2761 gcgaacgccg ggctttgggg gcggggatgc tggcgcgttt caccaccgag cctccggagg
     2821 aacaacggga gatcacgaga attttcgcgg ctctgcggga cggtcaaccc gttccgtccg
     2881 ccgtgaacgc caccgccggt cactggcggc ggggcgtgga ataaaccttc cggcatttgg
     2941 aaggaaagga gacccttatg gaacaggatc gtgcgctgcg cgtactggaa tttaccaaaa
     3001 tacgggaaat gctggctcaa cacgccatca ccgaggaggg caagcagcgc tgtttggaac
     3061 tgacgcccta ccatgatctg ggcgacgtga accacgccct tgcggaaacg gaggaggccg
     3121 tggtgctgct gacctatctg ggccagaacc cgctggtcag ctatccggac gtgcgggagt
     3181 ccatcgcc
//
LOCUS       DAWAEA010000145         3172 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4629_length_3172_cov_2.274623, whole genome shotgun sequence.
ACCESSION   DAWAEA010000145 DAWAEA010000000
VERSION     DAWAEA010000145.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4629_length_3172_cov_2.274623"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1015..1024
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2668..2677
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          650 a          937 c         1006 g          559 t
ORIGIN      
        1 tcggccccaa gggcttcacc ggcgaaaagt acggcggcgc gacctattgg gacaccgaag
       61 cctactgcct gccggtctac atggccgtgg ccggtcagga cgtggcccgt cagctgcttc
      121 tgtaccgcta tctgcagctg gacggcgcgt atttcaacgc caagcagcag ggcctgaagg
      181 gcgcgctgta tcccatggtg acctttaccg gcatcgagtg ccataacgag tgggagatca
      241 ccttcgagga gatccaccgc aacggcgcca tcgctcacgc cattttcgac tacgccacct
      301 ataccggcga cgaggaatat cccgtgaagg aaggactgga cgtgctgtgc ggcatcgccc
      361 ggttctacac cgaccgttgc cacttctcca agcggaaggg caagtacatg atccacggcg
      421 tgaccggccc caacgagtac gagaacaacg tcaataacaa ctggtacacc aaccgcatcg
      481 cagcctggag catcgcctat ttcctcgaac aggccgagaa ggccggggaa gcccgccggg
      541 ccgagctgaa catcacgccc aacgaaatgg cccatatgca agacgtggtg gaaagcatgt
      601 actatcccga ggacaaggaa ctgggcattt tcgttcagca cgacaccttc ctcgacaagg
      661 acttggagcc cgccgccgcg ctgcccgccg aggagcggcc catcaaccag cactggtcgt
      721 gggatcatat cctccgcagc tgctacatca agcaggcgga cgtgctgcag ggtctgtatt
      781 tcctcaatca cctgtacgat acggagacca tccgccgcaa cttcgacttc tacgagccca
      841 tgaccgtgca cgagagcagc ctgtctccct gcgtacacag cattctggcg gcccagctgg
      901 gctaccggga gaaggccgtg gaaatgtacc agcgcaccgc ccgcctcgac ttggacaaca
      961 tcaacaacga caccgtggac ggcctgcata tcaccagcat ggccggcagc tggcnnnnnn
     1021 nnnnatggcc cacggcttcg cgggaatgcg caccgtgaac ggcctgagcc tgaatccctt
     1081 cctgcccgac tgctggaagg gctacggctt ccagttccag taccgcggcc gggtcatccg
     1141 gctggacgtg aagcccggca aggccatcgt ccgtctgctg gaaggccagc ccgtggaaat
     1201 gaccctctgc ggtgaaaaag tcctgctgga aaaagaaacg gaacacgcca tctgagtgcc
     1261 tgaacccact taaaaccgaa cctcaaaccg cctcccgcat aggatgctgt aaggcatcat
     1321 ggcaggaggc ggttttcatt gagctggaac tggttgattg ccgggggcga cagccgcgcc
     1381 ctccggctgg cgcaaaggct gaaacggggc agaggacagg tgcagacgct gggacttctg
     1441 gaaggagatg aacagacggc gcggcccgag caggcggacg caatcctgct gccctatccc
     1501 tggtcggtgc gcggcgggct gatccccacg ctgacggggc tgacgctcca cccggaaaac
     1561 gtgctgcggc aggcttcgcc gggaacgctg gtgctggcgg acggcggtct ggaggatacc
     1621 ccggcgatga acgaggtgcg ccgccgggag ctgcgggtgc ggcgatatca ggacgcgccc
     1681 ggttttgcgg aaagaaacgc ggccatctct gccgaagggg cggtctttca cgctatgggg
     1741 gagacggacg ctacgctgga agggctgccg gtgatggtgc tgggctacgg cctgtttggc
     1801 cgggagaccg cccgccggct gaacgcgctg ggcgcgcgcg ttacggtatt ggcccgtcgg
     1861 gaagaagccc ggcagctggc tcggtcggat gggatgcgcg cctgtccctt cgaagcattt
     1921 atcggatttg cgggggagac gaagctgctg ctgaacacgg tacccgcccg gctgctgaac
     1981 cgggaacggc tgtcggcgct gccaaaggac tgcgtgctgc tggaactggc cagcaccccc
     2041 ggcggctttg acccggaggt ggcccgaagc ttgggcctac ggcagagaaa tttgcccggg
     2101 ctgcccggaa aatacgcccc ggacgcggcg gcggacgcgc tggaagcggc ctgccgctac
     2161 tttttccgac aggaaacgga gggacggacg cgatgaaaaa gaccaccgtt ggctttgcca
     2221 tgacgggctc ctactgtacc ttcgaaaaaa atctggccca gatggaagaa ctggtccggc
     2281 gtggctacga agtgctgccc atcttgtcct ataacgccgg ggagctggac acccgcttca
     2341 tgaccgcaga gcacctgcgg gagcggctgg tgtccattac cggccatgag cccatcgaca
     2401 ccctgcaggc ggcggaaccg ctgggcccga agaagatgac cgatctgtac ctgatttcgc
     2461 ccgcgtcggg caatacgctg gcaaagctgg tgaatggaat ttacgatacg ccggtgctgc
     2521 tgggggctaa gagccacctg cgcaatgagc gcccgctggt gctggccgtc tccaccaatg
     2581 acgggctggg cattgccgcc cagaatatcg gacggctgat ggcgtggcgc agcgtatact
     2641 ttgttccttt ccgtcaggac gacccggnnn nnnnnnngtt cgctggtggc ggattttgcg
     2701 cagattccga aaacgctggc ttccgccctg tgcggcgtgc agctgcagcc catgctggca
     2761 caggaaaatc gtgtcgggaa tggagaaatt ttgtaacaaa cttgcaacag aatgcgtttt
     2821 aaggtaaaat agtttcgcaa actttttcac accgatcggg aaagggggag gacggaggat
     2881 gcgtacaagg ctgacagctc ttttgctggt tcttttgatg gtttccggag tggcctttag
     2941 cgaggcggag accgtaaagg tagaggagat ttccgcggcg atgggagaaa gcaaggtctg
     3001 ctatccgcag ctttccggcc tctccgatcc gtcggtgcaa aagaagatca acgacgacgt
     3061 agtgcagtcc ggcgatatcc cgtccctgct ggtcaaactg acgacgggcg gcagtgtaca
     3121 ggccgattat cagtgcttct ggaatgaatg ggtcttttct gcgctgctga cc
//
LOCUS       DAWAEA010000146         3151 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4664_length_3151_cov_1.792636, whole genome shotgun sequence.
ACCESSION   DAWAEA010000146 DAWAEA010000000
VERSION     DAWAEA010000146.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4664_length_3151_cov_1.792636"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    642..651
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          679 a          873 c         1012 g          577 t
ORIGIN      
        1 gcagatggac caggcgcagc tgctgtttgg ggcgcttcgc atcggctttt ccgccattgc
       61 ggagcagtac cccgcgtatt ttcggctcac gacccgcaag gttcattgag ccacgacctt
      121 gatagataaa ttggagggta ttacaatgat gaagttgaat cttcagctgt tcgctcataa
      181 gaaaggtatg ggttctaccc gtaacggccg cgacagcgaa tccaagcgcc ttggcccgaa
      241 gcgtgccgac ggtcagttct ctctggccgg caacattctg gtccgccagc gcggcaccca
      301 catccatccc ggcctgaacg tgggcatcgg cagcgacgat accctgtacg ccaaggtgga
      361 cggtatcgtt cgtttcgagc gtctgggccg cgacaagaag caggtcagcg tttatcctgt
      421 ggctcagtaa tgacaaagcc agaagaccag tacggtttca aaccgcgctg gtcttttctt
      481 ttgtttgaat cataaggcga gggcacaggg gaaaaattcc gcctttctat ggacgcacat
      541 ggaacagata tggtaaaata ttccaagata ttttaggaga ggtgattaca tggattccgt
      601 gattcgggat atcgatctgg acccgcaggg gattcagaaa annnnnnnnn ntgaaagtgg
      661 tggtcagcgt tcatctggaa gcgaagaccg cctatctggc gcaggtgatc cacgaaggcg
      721 gcggcgaggt gtacgccacc ggctccaatc ctttgtccac gcaggacgat gtgtgcgccg
      781 gactggccag ccggggcgtg accgtgctgg cgacccacgg ctgcacactg gaagaatatc
      841 atgacttcca gtgcagggcc ctgtccgtga agccggacgt cattatcgac gacggcggcg
      901 atatggtgca tatcctgcac gaagagcacc ccgagtgggc ggtcaacctg cggggcggct
      961 gcgaggagac caccaccggc atcattcgcc tgcgcaaccg ggccaaggcg ggtcagctgc
     1021 ggttccccat gttcaatatc aacgacgcgg actgcaagca cctgttcgac aaccgttacg
     1081 gcaccggcca gagcgtgtgg gacggcgtga tgcgcaccac caatctggtg gtggcgggca
     1141 ccaccgtcgt ggtggccggg tacggctggt gcggcaaggg cgtggccatg cgggccaagg
     1201 ggctgggcgc gaaggtcgtt gtgaccgagg tcgacccggt gaaagccatc gaggcagtga
     1261 tggacggctt taccgtgctg cccatggcgg aggcggccaa ggtgggcgac tggttcgtca
     1321 ccgtgaccgg ctgcgacggt gtgatccgcc ccgagcatat gctggaaatg aaggaaggcg
     1381 ctatcctgtg caacgcgggc cacttcgacg tggaggtgga cgtggccggg ctgcgcgctc
     1441 tggcgaagga aagccgcgaa gtacggcaca atatcgaggg cttcctgctg cccaacggca
     1501 aaaccattta tctgctggcc gaggggcggc tggtgaatct ggcggcaggc gacggccatc
     1561 cggcggagat catggacatg agcttcgccc tgcaggccga gtgcgcccgc cgtatggcgg
     1621 agtgcggccg ggacatgaag cccgatctgt actccgtgcc ggcggacatc gacaaggcgg
     1681 tggccacccg gaagctgaca gcgctgggcg tgtccatcga cgttctttcc gaggaacaga
     1741 aagcctatct gggcgtataa tgcgcccgaa agcagagaag aacatacata agaaagggga
     1801 aatgttctga tgaagcgcag aatcggtatt ttgaccagcg gcggcgactg cccgggcctg
     1861 aacgcggcta tccgcggcgt tgcgcgcgct gcttatgaaa tgtttgacgc ggagatcatc
     1921 ggcatccgaa acggctacgg cgggttgatc gagggcgact atcaggaaat gcgcaagaag
     1981 cagttctccg gcatcctgac cttgggcggc accattttgg gcaccagccg cacgcccttc
     2041 cgcaatatgc ggatcatcga ggaaaacggc gtggacaagg tcgccgccat gaaaaagacc
     2101 tataaggacc tgaagctgga ctgtctggtc acgctgggcg gcaacggcac ccacaagacc
     2161 gccaacctgc tcagtgaaga aggcctgaac gtgatcggtc tgcccaagac catcgacaac
     2221 gatatttacg gcacggattt cacctttggc ttccacacgg cggtggatat cgccactgag
     2281 gtcatcgacc gcatccacac caccgccacc agccacggcc gctgcatggt catcgagatc
     2341 atgggcaaca aggcgggctg gctgacgctg tactccggcg tggcaggcgg cgcggatgtg
     2401 atcctgctgc ccgaaattcc ctacgacatc aaccgcgtgg cggagaccgt ggaggcccgg
     2461 gccaacagca agaagccctt ctccattctg gccgtggccg agggcgcgat gagcgtggag
     2521 gagtccaaga tgaagaagaa ggagcgggaa gcctaccgtg cttcgctgcc ctacgactgc
     2581 atctccaagc gcattgcggc gcagctgcag gaactggtgg gcgtggagga ccgcagcgtg
     2641 gttcccggcc acatccagcg cggcggcagc cccagcgcct atgaccgggt gctgtccacc
     2701 cagttcggcg tacacgcggc tcagttgatc cgggatgagc attacggcgt gaccgtggcc
     2761 ctgaagggca acaagatcat ccacaacgac ctgaaggacg tggccggggt gcccaagccc
     2821 gttcccatgg acgacccggt ggtcgtcact gcccggaata tgggcatcaa tatgggcgac
     2881 tgattgtttg aataaaacaa gcttcgcggg gcagctgctc cgcgaatttt tttattcaga
     2941 caaaataaaa gggccggttc tgctggaaaa tgcgaagcaa ctgcgggatc gacaactgat
     3001 aaggaaacct tgccacgcgg ccgggaatat gctatgctga ctgtcgaacg acgggaagtc
     3061 cgtccgaggg aggaaaggcc atgctgcgga cgctgttttt agtgtttttg aagatcgggg
     3121 cgtttacctt tggcggggga tacgccatga t
//
LOCUS       DAWAEA010000147         3146 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4674_length_3146_cov_2.447752, whole genome shotgun sequence.
ACCESSION   DAWAEA010000147 DAWAEA010000000
VERSION     DAWAEA010000147.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4674_length_3146_cov_2.447752"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    315..396
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    713..722
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2127..2136
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          514 a          987 c          936 g          607 t
ORIGIN      
        1 cctccgctgc ctatcgccgc tctgctcccc cgcagttggc gctgggcaga ctcaaccaat
       61 gcgagttggg atgttacggc gtaggttgga tggcttcgct gtatccataa acagacctgt
      121 ttcccaactc acagctggtg gtaggcttgc tcaaccggcg cagcatgtat ttttttgcaa
      181 cagcaaaggc cagcatggat ttgaaaccaa gctggccttt ttagcgagcc atgaggcccg
      241 tcgccgaatg gcggtctttc ctattcaact aacaacgaat ccacatcaga cccgcgagcc
      301 gcgaggcccg tcgcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngggg cactgcccct gccttagtgg
      421 ccgatggctt cgtggaagga ttcgaagaac tcggggcagc tccaggtgcc gcaggcgctg
      481 accgtgtcgt cgcccaccag aagctggagg gtcagaccgt tggtcaggcc gatgtgcagg
      541 gagccggaac cgctcatgcc cgtgctctgg taggacgcat tgtccagaag ggtctgcatc
      601 agggcctgcg cgtccgcgtc ttccacggtg ccgacccctt ccagcgtcat ccagctgacc
      661 tgagaggccg aacagagcgt gtcgttcaga ctttcgctgc cgatggtggc tgnnnnnnnn
      721 nngtgtgggg tgcagggggt caccccctgc ccaagtcggt ggcaaggacg gagagctggc
      781 cgtaacgggc ttcggcggca agaccggcca aaccgtgaag gccgcaggca gcctgtagga
      841 cttccagatc ggtcatgctg taggcccccg cctgacggcc cgccagcagg gcacagagaa
      901 cgccggtcag cgcatcgccg ctgccgccct tggccatggc gggcgtaccc agtacgttca
      961 gggcctcgcc ctccttggcg atcagatgac tgacggcatt tttcaaaaca atggccgcgc
     1021 cgtattgccg ccgcagttgg cgcgccgctg cttcctgatc ggctaagacc tgcgatacct
     1081 cgcagcgcag aagccgcgcc gcttcggcag gatggggcgt tagtacgcag gaagataggt
     1141 catagccctc gtccgaatga gaaagcagat tcagcgcgtc cgcgtccacc acggtaggca
     1201 ggggacggct gtcctgccgg cggcgcagag accccagcac acgtctcacc atctcctgcg
     1261 cccatgggcc ttgccccagc ccgcagccca tgcccagaca gtccgcctga ttcagggcct
     1321 tttccagcag cagaaacgcc gacgttgggt cgtcctccgg caggggcaga caggtggcgc
     1381 aggggcataa ggtctgaatg atctgccaga tgttgtcggg acaggccacc gtgaccagcc
     1441 cggctccggc ccgcagggcg gcgccggcgc tgatggcggc ggctcccgcc atgcctttgc
     1501 tcccggccaa tagcaccacc cggccatagg ttcccttgtg gctcagatgg cggcgtggcg
     1561 gcagcagacg gcgcaggtct gatggctcca gcacgtcaaa cccggcggaa gtcccctcgt
     1621 ccgaaggaat gccgatagag accacgtcca cctgcccggc aaaatccagc ccgtaccccc
     1681 gataaaggcc gggcttgggg cgatggaacg tcaccgtgcg gtcggcacgg acggcacagc
     1741 ccagaatcac gcccgtggtc ccgtccagct cggagggaat gtccactgcc agcgtgggga
     1801 cgcgctcgtg ctcgttcatc atggcgcata gcgccagcgc cgttccggtc agcgggcggc
     1861 tcaaacccgt gccgaacaga ctgtccacaa agcagcagac gttctccggc agggacggca
     1921 gcgggccgtc cgtcagacgg acgacctcca cggcgggggc ttccgcctgc agacagcgca
     1981 gctgatgctc cgccgcgtcg tttagcgtgc caggcaaaag ccagcacacg gcccgcagtt
     2041 ccgggcggct ggcagtcagc agacgcatcg ccgccagacc gtccccgccg ttgctgccgg
     2101 ggccgcacag gcacagcacc gttccgnnnn nnnnnnccgc cgccgcgtcg gccacatgcc
     2161 gggctgcgct ctccatcaac tggagaccgc tcgtcccggt gcggttcata taattttctt
     2221 ccagacgctt catttcgtct ggggtgatgg tcttcagcaa ggttctttct ccttgattcg
     2281 tcagccgttc atgcccggat cggcgttcgg gtcgaccgtg ggcagaggcg taacggcggg
     2341 atcgggcgtg gcggtctgcg gcgcgtctgt cgtgaccgca tcctgcgccg tgtcggcagt
     2401 gggctgcgcc gtcgcggcgt tctgctcgtt agagggccac gggtcgcggt ccacatgcat
     2461 cgtgcaggcg ctcatcaggc acaggcagca aagcagtgcc agcaccagac agattttttt
     2521 cattttcatc ttcggttcct cttttcgggt cagttttcgg aggacgaatc gccggtctcg
     2581 tccgcgtcgt gggattcctc cggcgcatct tccttgggcg tttcttcctt ggaggtcagc
     2641 gcgcccttca aggcctgcac ggccttgtcc agcccggtcg ccaccagtgc cgcgccctta
     2701 gccagatagt ccgccgtctg gctaaccgcc tgacccgcct gttttttcag attttccgtc
     2761 cgttccgcaa attccgggct ggacagggta tcattcagct tctgtccgaa ttgctccaca
     2821 gcggcgctga cgttctggac ggtctcctcg gccttgcggc aaacttcgtc cttcagatgg
     2881 tgcagcgcgt catacaccgc gcccatcgcg tcgtctacgg cctgtttggc cttttcccaa
     2941 tcgttgttcg cgttcatcgc ttccatcctt tccgcagccc tctttttttc ggaaggctgc
     3001 gcctttatta taatgtacgg gcagggaaag tcaaattcct ttccaatctg ccggaaatat
     3061 ggtatcattt gctgggaaag gagtgcattg gaatgattcg caatatcgtt tttgatatgg
     3121 gcatggtgct gctgcgttac gagccg
//
LOCUS       DAWAEA010000148         3133 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4693_length_3133_cov_1.918129, whole genome shotgun sequence.
ACCESSION   DAWAEA010000148 DAWAEA010000000
VERSION     DAWAEA010000148.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4693_length_3133_cov_1.918129"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1641..1839
                     /estimated_length=199
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          546 a          887 c          904 g          597 t
ORIGIN      
        1 ggttaaaacc gaaggtcagc gcgccggaag cgttgcggcc cgtagagaac aggccgaaga
       61 acaccagtcg gatgccttcc catgcgcttt gcaggccaag gcccttggtg gtcaggccca
      121 cgatcaggat caggacgctg cccgctacca gaccgatcag aatggagatc agggaagcca
      181 gcaccgagcg ggtgccttcc ttttgcagaa agtttttcat gcctccgcct ccttctcctg
      241 ccgcttggcg ccggccatgt acaggcccag ctcctccacg gtgacttgct tgggatcaaa
      301 ttcgcccacg atctggccct cgtacatgac cagaatgcgg tcggaaaggt tcatgacctc
      361 atccagctcc agcgagacca gcagcacggc cttgccctcg tcccgctgct tcaccagctg
      421 gccgtgaatg tactcgatgg cgcctacgtc cagaccgcgg gtaggctgca ccgccacgat
      481 caggggcagg ttgcggtcga tctcccgggc cacgatggcc ttctgctggt tcagcttttc
      541 ggcgtacttg cgggtctcat cgaaacgaat gaagccgtgg ttctggaact gcttttcctc
      601 aaagcgctgc aaaaccatgt tctgctccag cgtaaagtcc agaatcaggc cgtgcttgtg
      661 ccggtcttcg ggaatatggc tcatgcccgc ctcgctgtgt ttgcggatgg agacatgggt
      721 gatatcgtgc ccttccagcg tgatcttgcc gtgagagggc ttttccagcc cggtgagggc
      781 gtagaccaga tcggtctggc cgttgccgtc gatcccggcg atgcacagga tctcgccggc
      841 acgggcagtg aagctcacgt cgctgaccgc gtcccgcttg tagagcttgg agggcacgtg
      901 cagatgctcc acctccagca caggcttgcc gggatgggcc tcgtccttgt ccaccaccag
      961 ctgcacgggg cggcccacca tcatgcggct cagttcttcc ttggtggtgt tggcggtctc
     1021 cacggtgccc acgtagcggc ccttgcgcag cacggtcaca cggtcggaaa cctccatgat
     1081 ctcgttcagc ttgtgggaaa taaacaggat gctctttccc tcccgggtca ggccccgcat
     1141 gatctccatc agttcttcga tctcctgagg ggtcagcacg gcggtaggct cgtcgaagat
     1201 cagcacctca ttgtcgcggt agagcatctt aagaatttct acgcgctgct gcattcccac
     1261 ggtgatgtcc tcgatcttgg cgtccagatc caccttcagg ccgtaacgtt cggacagctc
     1321 ctgcaccttt ttgcgggccg ccttcttctg caggaagccc attttggtgt cctccgctcc
     1381 gaggatgatg ttgtccagta cggtgaacac gtcgatcagc ttgaaatgct ggtgcaccat
     1441 gccgatgtgc aaagcggtag cgtcgttggg gtcgttgatc tgcacctcta cgccgtcttt
     1501 tttgatgacg ccttcttccg gctgatacag gccgaacagg acgctcatca gcgtcgattt
     1561 gcccgctccg ttttctccca gcaaagcatg gatctcgccc tttttcagct gcagggtcac
     1621 atcatcattg gctttaatgc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt cttaagagtg tcaggcctga
     1861 cggaaaaacc cgtagaaacg aggcgaaaca atggacagac aggagctggg gcagaggatc
     1921 gtcgattgga cgcccctgct gtatcatgtg gcgtgcggcc tgctttccga tgagagcgac
     1981 cgggaggacg cggtgcagtc ggccatcgaa aaggcatggc gcaaggcgga ttccctgcgg
     2041 gagccggaca agctgaagcc gtggctcgtg cgtattctgg tcaacgaatg ccacgaggtt
     2101 ctccggcgca gaaagcggga aattcccacg gagaccctgc cggaggaaag cgcccccccg
     2161 ccgcaggagc agcgcgagct tcgagagctg gtcatgagcc tgcccgaaaa cgagcgtttg
     2221 cccatcgtgc tgcattatct ggagggtttt tcaacacagg agatcgcgtc cgcgctgcgc
     2281 tgccccaagg gcaccgttct ttcgcgcatg agccgcggcc gcaggcaatt aaaacgcttc
     2341 ctgacggagg aatgagcgat gaaagattgg gattcccttt ttggtgatgt gccggagagc
     2401 ttttcccggc gcgtccgtga tacggtggat cggctggagc ggcaggaaag cgcccgccgc
     2461 ccggccttcc ggctgcggcc tgtagccgtg ctggcgctgg cactctgcct gctggccgga
     2521 acggcggcgg ccttgagcgg attgggcgtg ctggacacgc tgggccggga gctgcgggcc
     2581 tatctgcagc ccgaagccaa agaactggtg cagaccaccg tttcccagac ggccagccag
     2641 ccccggcacg cggcctttac ggtggaagaa gctgtccacg acgggcggca gatgtacctt
     2701 tccgtccggg tacacccgga aagcgacgct ttgctcatgg acttcaacgc cgaggcttcg
     2761 tggagccgca gctggccgga gcatcctgcc cagaccgacg acggctcgga aaccttcgct
     2821 caagcggcgg ccgtgcaggg caaggaactg gttcgggccg actgtgagcc tgtggatgaa
     2881 aacggaacgc tgctggatcg ggccgtcgag gtacattacg acggcgacga cctgctctac
     2941 aacctgtcct ttgcgtcgga gagcggaaag gcctccctgc gcctgttcac cgacgaggtg
     3001 tacagcgagg actggaaaca gagccgcagc ttcggtacgc tggacttgga ggtcaacgtg
     3061 accgaacggg gcaaaatcta cactgcggat actcccgttg tctttgcgga cggcgagctg
     3121 gaattgaccg ggc
//
LOCUS       DAWAEA010000149         3128 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4700_length_3128_cov_2.333225, whole genome shotgun sequence.
ACCESSION   DAWAEA010000149 DAWAEA010000000
VERSION     DAWAEA010000149.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4700_length_3128_cov_2.333225"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    524..533
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2055..2064
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          666 a          953 c          861 g          628 t
ORIGIN      
        1 cccttgagca gatgagaggc cttaggatcc agccccacga agggttcgtc catcaggatc
       61 agcttgggtt cgtgaatcca tgcggaaata atggccagct tctgcttcat gccgtgggaa
      121 taagccgcaa tgggctgccc cagatcgccg gtcagctcga aaagctcggc gtatttccga
      181 atgcgttcct gccgaacgtc cgcctctaca gcgaaaacgt ccgcgatgaa gttcaaatac
      241 tggatgcccg tcatgaactc atacaggtcg gggttatcgg ggatataggc catctgccgc
      301 ttgcagcgga gcgggtcggc ctgaagggat acgccgccga tgagaatctc tccttcgtcg
      361 aaggcctgaa tgcccgccac ggatttcagc gtggtggtct tgcccgcgcc attgtggccg
      421 atgaagccgt agatttcgcc cggagcgata tgtagactca gatcgtccac cgcttttttg
      481 tcgccgtagg ccttggtgag atgttgaatt tgaagcattg cttnnnnnnn nnncttttct
      541 ccttgcttgc atcggatttt ttcgtttcca tagaaaaacc gtccaaacga tttcatttct
      601 gaacatcgtt ctgacggtta gtataacaca cgttgtgcga attgcaatga tgattcgtcc
      661 tattgtcaaa atcagtgtct caaaacgtcg ctttcgcgtc cccgatcgcc tttcgaaagg
      721 ctgcgtcccg gtcgaaggcg ccgtccacga agccgggcac ttccttcagc gccttgcaca
      781 ggctcaggct gaagccggtc agaatctgct gcaccttctg ccgggaatag gggcagttgc
      841 ccgtgaccgt cagcgtacac agactatgca ccgtcagcca catatcccgg aaatagcggt
      901 cggcagtagg gccgtccatc cgataggtct gcatcagtcg gtcccgcacc tgttcccgta
      961 cccggcccat ggcgtccagc gcgctgcact gtccctcttc ggggcgagtc atgtacaaca
     1021 gctgatacag cttcggttcg tcctgcgcaa actgcagata ttgaacgccc acgcccagaa
     1081 acggcggttc catcgccagc cctttttcca catactgccg gaaaatattc tccgcaaaca
     1141 gacgcacttc ttttttcagc tcgtccatcg tgtggtaata ggtaaaaatg ggccgggtag
     1201 aggtgcccag caccacggcg acctcccggg cggacaccgc cccgatgccc ttttcccggg
     1261 cgacggtcag cgcggcgttg gtgatctcgt ctttggtaaa cttcggctta ggaggcaatc
     1321 cgatcatcct ttctccgtca aaagcaacgc gtccagttct ccggtcatgt tgcaaaacat
     1381 atgttatata acaagatgat tataggatct gcctggcggg aagtcaagct aaatcaatgg
     1441 aaaaaaaccg aaccagcgcc tgaaaggatc atcacctgcc gttcacaaat tctttgaaaa
     1501 atgttcgtct ttcctccaac ctattttcct ttttttctgc tatcctttga aacgtaccga
     1561 gggaggaaac caaaaggctc cccggtagag atcccaaaag gagaaaatca ccatgaaacg
     1621 gaattggaaa cgaatcaacg ctttgttttt agcgatttgc cttttgtttg tatcgtcctt
     1681 cgccctggcg gagggaaacc ccggggaccc gccggacgga cagccgcctc agggacaggg
     1741 tggaaccccg ccggaaaagc ctgacggaga agcccccggt gaacccccgg ccggtgatat
     1801 gggcggcggc ccgggcggca tgggcggccc cggcggcagc agccagcccg actcctacgc
     1861 cgccgtgcag accgtatcgg aagacacgca gctttccggc gtaacgctgg attccgtggc
     1921 agcggatgaa aacgcgctgc tggtcacggc gggcaacgtg caggtaacgg acagcaccct
     1981 gacccgtaac tccacggatt ccaccggcgg cgattccgcc agcttctatg gagtcggcgc
     2041 ggcggcgctg gtgannnnnn nnnnctccac catcaccacc gacgcgaacg gcggcgcggg
     2101 cgtattcgcc tacggcagcg gcgttgctac cgtagcggac actaccatcg acaccacgca
     2161 ggacacctcc ggcggcattc atgtggcggg cggcggcact ctgtacgcct ccaacctgac
     2221 cgttatcacc cgtggaaact cctcggcggc tatccgctct gaccgtggcg gcggcaccat
     2281 ggtggtggac ggcggcagct acacctccga gggttccggc tctcctgccg tatacgtgac
     2341 ggcggatatc accatctcca acgcgcagct gacggccacc ggctcggaag ccctttgtct
     2401 ggaaggcctg aacagcgtca gtctgaccga ctgccagctc agcggcaaca tggccgacct
     2461 ttcccagaat gacaacacct ggacggtcat cctgtaccag agcatgtccg gcgactccga
     2521 agtgggcaag ggtaccttca ccatggagag cggctccctg acctctctga acagcggcct
     2581 gttctacacc accaacaccg aaagcgaatt taccctgcgc aatgtgcaga tcaccgcttc
     2641 ggacgattgc gagtacttcc tgcgctgcac cggcaaccag aaccagcgcg gctggggcca
     2701 gagcggccag aacggtgccg actgcgtttt caccgccgcc cagcaggaaa tgaacggcaa
     2761 cgtgatctgg gacagcatct ccaaccttga cctgtcactg acggagggca cggttttctc
     2821 cggcgcggta ctggatgacg aaagctgcgc gggcaatggc ggcaacggcg gatgcaccct
     2881 gaccattgat gaaagctcca gctgggtcgt taccggcgac agcgtcgtga ccacactgaa
     2941 ctgttccggc tccatcgtgg acgcggaggg ccgcaccgtc accatcgtgg acagcaacgg
     3001 caacgtgctt tccgagggtg aaagcgaata taccatcacc gtgaacacgc tgcagagcac
     3061 cgcagtctga aaaatcccat cgaaaaagcc ctgattggcc gtcattcccc gcgaacagcg
     3121 ctctgcgg
//
LOCUS       DAWAEA010000150         3121 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4714_length_3121_cov_1.851924, whole genome shotgun sequence.
ACCESSION   DAWAEA010000150 DAWAEA010000000
VERSION     DAWAEA010000150.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4714_length_3121_cov_1.851924"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    402..467
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          614 a          982 c          872 g          587 t
ORIGIN      
        1 ccttactcct tgacttcttc ctgttccttt ttcaaacggc ggcggcccag caggaaccac
       61 acgatggacg cgcagaaggc cgaagccgca tcccagatca ggtcctccat ggtgtcctgc
      121 agagcttccc ggcccaccag ctccacgccc tgcgccgttc ggaatttttg catgttcaga
      181 cccagcaacg cgtcgaagga atattcgtaa atttcccaca acgcgcccaa cgccactgcg
      241 aagcagaagg cgaacatcgc cgtaaatccc gggctcagcg acacggaaat tttgccggag
      301 cggttcagca cgtccacgat ggtaatgccc agaaggctca gcatcactgc ggagaagctg
      361 tgcagaagcg tgtcccagaa gggcaccaga tagtagaagt cnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngta caggaacata
      481 tagtactgaa cgtacagcac gtccggcacg ggcagtttcc agcgccgggc cgcccgggag
      541 ggcagcagca tgaccaccag acccagaatg cactgggtga gcatgagcag ataatcgctg
      601 cgggtcttac cctccgtgcc tgccgggagg gactccggcg cgcccgccag ccgaatggcg
      661 gaataaacag atgctgcggc cagcgacaca aacaggatga tctcgaaaac gcgggatagc
      721 gtatggtgac ggtttgcctt ggactcgggt gtttttttca tcgggcggtc tccttgcaca
      781 gttcggggat aaagtcagtc tgttcacctt ccgcacggcc cattccaccg gcagaaagca
      841 ctttatcagt atacgggaat cagtgggaat tgtcactagt cagaaatccg attgtgcaca
      901 aatccagcgc aaaaatcccg aaacaggcaa agcccgttcc gggaaaactt actattccgc
      961 attttttcgg atcagcgggg caatcagccc ggcaaagccg tggcagcagc tggcccgggg
     1021 actgaggaac tcccacaacg tgcccgtctg ccgggccatt tcgccatagg aggcgcggat
     1081 ttcgtccaat acctgcccct tgcggccgta acgatccagg atcagcatcc gcagcagctt
     1141 gccgataaaa gcgttggagg ggtacagccc tgaggccgcc agccacgcgc cgtccggggc
     1201 aaagaggttt tggaacaatt ccggcgtgtc gcccggggtg gacaggttga aaaagaacgc
     1261 ataatactgg caggtctccg tgcattcctc cgtcacggtc aacccgccgt cttcctgccg
     1321 caaggcccgg tcgtgccaga gcgtgccgtc gaaaaacacg tcccgcaggg tatgccgtac
     1381 ccgcgccgct ttttccgcca gcgcggggcg gtcgtacagc cgtgccaccg cttccagcgc
     1441 cgccgcgtag agcatattgc tgggggcgtt cacatcctgc accagctcgt tggccttaga
     1501 ccactccacg aatacccacg cttccagctt ttccagcagc cccagctcgt tttcaaagcc
     1561 ctcgaaataa tgcagcgcgt ccaacaggtg tttttctgcc agttccgcca tttcccggtc
     1621 gccggtgcgg cggacgtact cctccgtttc cagcaggagc cacagcatcc agttggggat
     1681 aaagttgccg ttgcgcgggt cggaggggta gcacatgggg atcacgcccg gtttcagcca
     1741 cggatagcgt tccggcagga aatagttttc cagataggtt ttttccgtcc gattggcccc
     1801 ggtcagcgcc cattcggccc gccccatgaa gaagctgtcg cagctccagc ccgcccgttc
     1861 ccgggagggg cagtcggtaa aaagatcgta gctgttctgc cgcagggtct ccacggcggc
     1921 atggtcgatc tgccgaagca ggccgtctct tacgtactct ggcagcggcg gataatcctc
     1981 cgccggggcg gcggcttcca gcaggtgcag acggcggatg gtgcagcgac cctcggcgca
     2041 gaccagcttg aggtattgca tcgctgccgg ttcaaaggcc gtgaagcgat gaacaccccg
     2101 gctgaacgtc agcttcatag ccccgatcag gctcattcgg agaggattca cgtccccatc
     2161 cgtcaaaatt tcatcataca gggcgtacaa aacgccgggt tcatcccatt cgatttccat
     2221 cgtaaggaag cctgtttttt ctccatcaaa acgtagccac gcgccgtctc cctttttcag
     2281 acgaaggggc agcgcaaaag gcacggaaac gggcggttcg tgattcggca gaatggcgga
     2341 aatgtcccgg gtcagcagag tcgtgcactc ctcccggcgg tagccgccga tcagctcccc
     2401 tacgccggtg gtgcagcggt cgtcccatgg gtgcgcgtgg acaaaatccc acgaaacggg
     2461 cttctgcttc accagccagc ggggcgcgat ctccggcagc gcaggtaaag accgccgcgc
     2521 cggaatcagc cgcttgggct cgcagcctgt cagcggttcg gccattcccc gcccgctgcg
     2581 ccagccgttc cagtctcccg tcaggtcgta gacctcgctg aacccccgct gatagctgta
     2641 gcgttccacc ttctgctccc ggtagggcag acgcaggccg acgaatctgc cgccctctgg
     2701 aagggttcgt gcgatacatt ccccgtccgc ccagacctcc gcctgcagga agggcggctg
     2761 atcggccaga aaaaacgtgt tcacccgggg cacgttgatc tcgatcgcca gatggctgat
     2821 cctcggcccc gctgcgcccg tcaggtcgat ctcgctgacc cggtgccagc cgtgggcgca
     2881 gcgcaccggc ccgtaggcca gatgttcccc gttcagatag acgtgataat gcccggctgc
     2941 ggccagcttc agcacggcgc tgtgctttcc ttccaaactg agcggcgcat aaaacccggc
     3001 ggtcaggttc ttttcgtcct gccgcccctg cagccatacc ggcaaagact gttccatcat
     3061 ggagcacgtc ctcctttgcc ctgtgcttct tccgattttc ccacaattta gcatatccca
     3121 g
//
LOCUS       DAWAEA010000151         3120 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4716_length_3120_cov_2.528548, whole genome shotgun sequence.
ACCESSION   DAWAEA010000151 DAWAEA010000000
VERSION     DAWAEA010000151.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4716_length_3120_cov_2.528548"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1795..1804
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          642 a          937 c          865 g          666 t
ORIGIN      
        1 tatgcgccta cgggcgcacc aaagggcttt ccgctcgtcc tttggaaacc ttcgggcacc
       61 gaagttggtg aataacccat gggacgacaa gtcccaatcc gcgccgtagt cggcgcgtca
      121 tgccccatcc aggacaaggg atgtgcgcct gtaggcaccc caaaaagctt ccctcttttc
      181 tgcgcaaaaa acaccggcag gttcccccac cggtgcaact cttttaactt tttccttact
      241 ccatcggctt aaacccttcc cccagcactt catgcgcgtc gctgataaac acaaacgcct
      301 gcggatccat ccgatgaacg atgttcttca gccgccccaa ctcctgcgcg ctgaccacac
      361 agagcaccac gggacgctcc tgcccgctga acccgccctt ggcgtccagc atggtcacgc
      421 cccggccgat ctcgtccatc agagcctgtt tgatctcctt cccgtgcagg gagttgataa
      481 aacaggcctt ttccgtcgtc aggccgttca tcaccacatc gatgagtttg gaagaggaat
      541 agaggcagat gaaggcgtac agggcgtact ccgcctgaat ctggaagccg gccagcagca
      601 ccaccagaca gtcgatgatg aacaggatca cgcccacgga gagataaggg atcctttggt
      661 gaatcacccg ggcggccatg tccgtgccgc cggtggtggc cccgcccttg agaacgaagc
      721 ccaaaccaac gcccagcagc acgccgccga aaagggagcc cagcagcgga tcgtgggtca
      781 tgggctgcag gggcaggaag tcgatcagca gggaaaagat caccgtcgcg cccagcgaac
      841 gcacggcaaa ggtgcgcccc atggagcgat agccgatcag aaacagcggg atgttcatca
      901 aaaggctagc tgtgcctacg ggccagccga acagatagtt gagcacggtc gccagaccag
      961 tcacgccgcc gggggcaatg tgattgggca ccagaaacca cggataggcc atggctccca
     1021 gcagacagcc cagtaagatc tgcccccagg cagtgatccg ttctttggtg aaccattcct
     1081 tcatgggttt tgttcctctc tttctccctg ttccaaggga tagggacgca tatccagcga
     1141 atgccgcagc gcttcctcta cctgttccag cctgcccggc ggaagggttc ccaaccgccg
     1201 atccagcctt gttttttcca gcgtgcgcac ctgttcgcac agcacggtgg acggctggcg
     1261 cagcccgccc tcccccattt ccagccgcac atgggtcggc aaaggcggct tgcgggggct
     1321 ggcggtcagc ggcaccgcga tcaccgtagg cgcgcggcgg tttcccaggt cattttggac
     1381 gatcagcacc ggccgccgtc cgccctgctc gctgccgacg acgggatcca gccgcgccat
     1441 ataaatatct ccgcgtttca cgcatttcac tctcccggac ggcaaaaatc aggccgcgct
     1501 aaggaaatgc gcggctcaag ccccattcgg agcccatacc gcccttttca ttctatgcgt
     1561 gtgataacga gcgtgcgctt gggttatggt aacattttcc cggctttccg tcaaggtggg
     1621 gaaaagccgg agttccgcgt ccagcctttc ttttcccctt gacatgcccc attcgccgcc
     1681 ttatattgat acctgtttga ccctcatcaa ggaggcctcg tcatggaact gttcatcctg
     1741 attctggaga tcgtaggcac ggtgtcgttc gccctttccg gcgcgatgac cgggnnnnnn
     1801 nnnntctgga tcccaaatat gtgctgatgt ccattgtcac gtcgctgttc gtgttttccc
     1861 cggtggtgcg gcgggtgctg ttccacagcc gcaagctgta cgacgcgctg atgctgatct
     1921 ccgactctgc cgggctgggc atttttacgg tgtacggtgc ccgggtcgcc attggcgcgg
     1981 gctatgagga aaacctgttt ctggtgctgt ttgtggcggt gatcaccggc gtgggcggcg
     2041 gcgtgatgcg cgacctgttc gcgggcgacc ggccctatat cttcgtcaag cacgtgtatg
     2101 cctgtgccgc gctgctggga gccattgcct ttgcggcgct gaaaccgctg gtagggctgg
     2161 acggtgcgac cgtcatcggc ttcgcgctga tcgtcgtcat ccgcttctgc tccgcccgct
     2221 tccactggag cctgccgaaa gcccgcttat aacgccttcg gaaagtccga accgccgcga
     2281 tctcccgcgc cgccttgctt tgcgcctttc atggaaaaat tgtgggtgaa ttgtggttaa
     2341 attgttgaaa aaaatttttg cccatggaaa tcatgtggaa aagatttgga aacttagacg
     2401 acgtctgaat ttgcgcgcgc cccgttttcc actcccccca catatagagg gattgtatgc
     2461 tatatttagt attgacgaaa ggtcaaatcc accatataat gttgtttgtt tccggctggt
     2521 gacgatttct gtcggaagcc gagccacaaa ttccatactt gaaaatagaa ggagccctga
     2581 gatcatgatc atttccatca agaagaggga cggacgtgtc gtgccctttg acccgagcaa
     2641 aattgagcac gctattgccc attgttttat gggaagcggc agtcagaaaa gcgatgaaac
     2701 ggctcaggag cttgcggcgc tggtcgtctc tcagttggaa aacgacgaga acatcccttc
     2761 tgtgccttcc gtggagcagg tgcaggacgt ggtggaacgc gtgctgatcg aaaagggctt
     2821 cgtgcgttct gccaaggcgt acattctata ccgcgccgaa cgcagccgtg tgcgcgagat
     2881 gaacacccgc ctgatgaagg tgttcgagga catcgccagc aaggacgcgg tggacagcga
     2941 catcaaacgc gaaaacgcca acatcaacgg cgataccgct atgggcagta tgctcaagtt
     3001 cggcagcgaa ggcgcgaagc agttctatga aatgtacgtg ctggatcccc gtttttctgc
     3061 tgcccatcat gacggggaca tccatatcca cgacctggac ttctacaccc tgaccaccac
//
LOCUS       DAWAEA010000152         3120 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4718_length_3120_cov_1.944535, whole genome shotgun sequence.
ACCESSION   DAWAEA010000152 DAWAEA010000000
VERSION     DAWAEA010000152.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4718_length_3120_cov_1.944535"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    790..799
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1908..1917
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          392 a         1137 c          890 g          681 t
ORIGIN      
        1 atagatatgc agtcctacaa cggcggcgca tttttcaatg gcctgcacgc tgtcgtcccg
       61 ccactgctcc cacgcctccc ggctcttgtc cagccgctcg atctccgcgt cccgcatggc
      121 ctcgtagcgc ttttccagtg cggagaccac cccgtccgcc agctggtcga tgctgtccgc
      181 gtcccgctcc ctgatctcct tcttgaggtc gtagaccttc tcttcccagg ccatgatctc
      241 ctcggcggtc atctggtgcg gaaagtttca ccccggcggc cagcagggcc gcctgtgcct
      301 ccttggccat ggcgataatg gcttccagcg cggcaatggc ggggctgttg tccacgtcga
      361 tgttgccctg catgatgagg ctctgccggg tcgcctccgc ccagctgatg acgctgctca
      421 ggtcgctggc catgctggcc gcggccgcgt ccacgctttc gtctgcggcg gtgacggcgt
      481 cgtccagtcc ctcgaagttc ttccccagcg ccgccgtgga gattcccgcg gccttgcact
      541 tagcgcgcag ctgctccgcc acccggttat attctgcgcc gttcttctcg cagctcttca
      601 gctgggcggc cagctccttc acctgctgga tggccgtttt ctgcctgccc agcgtctcga
      661 tctgccggtt gcacttctcc acgctggcca tggacgcgtc aaactgcttc tgggtgttct
      721 ccgcctcgct gcccatctcc ccgaaggcgt ttgcgtaggc ggacgcggct tccgcgccct
      781 ccgccaaagn nnnnnnnnnt cagggtatac gcgccgctgg ccgcgtcctc cacccgctgg
      841 ctgaaatcct ccacgccggt cacggactgc atcaggttat ccagcagggt ttcgacggcc
      901 acgccgcttt cctcgctcag ccggttcagg aagctcagca ggctgttgtc tccctccgtc
      961 aggaaggccc agctttccag cagtgcctcc tgatcgaagc tctccccgct cagggccttg
     1021 ccgatctcct ccgtcagcgt gttcatggcc ttgcctacgt ccgcgtcggt gatggcgtac
     1081 tcgaaggcct gcagcatctt cgtcacgtag tcgctggcgg cctccgcgct gtcaaactgg
     1141 ctcaggtcga tctggaacat ctgacgcagc gcgtcggcgg tggcctgatg gtagctgtcc
     1201 ccccgggcaa ggatcttcgt ctggatctcc tgcgtgccta gctggtacca ctggacgaac
     1261 tcgctgtcgg cctgccgcag ctgcgcaagc agctcctgcc gctggcgctt cgcgtcgtcc
     1321 agctcctgct gggaaaaaat gctgtcgctg ccggaaagcg cccggtcggt ctcgatctcg
     1381 tcgatcagcc cgttcaccgc tttcaggtcg tcggccacct cgcccagcgc ttcgccgtag
     1441 tccttcatgg cctttagccg ctgggtgccg gtgttggcgc tctgctgctg tttcaactcc
     1501 tgcagctccg cctgttgaag ggcccgcagg ctggcggcgt actggccgtt ggcgtctgcc
     1561 gcgtcccggg cgttgtccgc cgtcacgccg taggcgtccg ccagctcgtt ctggatgcgc
     1621 accatttcca gatgttcggt gtagtccaga ttttccttgc tggtcagctc gttatagcgc
     1681 tgcaccagct ggttcagccg gtcgtagcgg gcctgctgct ggtctaccgc gtcctgactg
     1741 gcctgcagct gcgccgcttc cgcttcctcg gcggccttcc gggcgttctg tacggcggtc
     1801 acgatgcccg caatgatgcc cgccacggcg gccagccccg ccagccagcc catgcccgcg
     1861 ccggtcaggc tcaggctgga cagcagcttt ttcaccgtgg caagcccnnn nnnnnnncac
     1921 cagccccgtc atggccgttg cggcggtagt cagccccgcc gtcacctccg gcgcggcttc
     1981 cgcaatgccg cccagcgccg tcaccacgcc ggtctgtacc tccgtggcca ccccggcaat
     2041 gccgctcatg gcgtcgccga agttctgcca cgccaccttc ccggcggcgg ccgcctgagc
     2101 ctgactgccc gccagcgtgc cggagagctt ttccagatcg cccacctgca tagccgtttc
     2161 ggccatgatg ccctcgtatt cggcctgcac cttctgggcc tgggtcaggc tggtggtggt
     2221 cacgccgatc ttcttggcgt aatcctccca catcttcgcc acattcttgg tcacgcccgc
     2281 gttgtccacc agaatgctgt tctcgttctt gatgccctcc gtggcggtgg tcacggcctc
     2341 ggacagggtg tagttggcct gacggccaaa gctggccgcg tccttcaggc gcaggatggt
     2401 gttcgtggcc tgatccagcg tatacccgcg gctgagtcga aaaacgcgtc cgtcaccccg
     2461 gccagcgccg cctgcatctg atccgcgccg atgttctggg cgctggaaac ggattccagc
     2521 ccctgcatgg cggcggtgta ggcgttgctg gcctcgatgc ccgtctggat ggccccggtg
     2581 atcttgctga acgtcacgcc cgccgcggcc gccagcgacg cccacgcggc ggactgggcc
     2641 gcggcgctct tttccgtctg ctgctgagcc tcctgcgccg tccggcggat ggagctggcc
     2701 agctcgtcca gcccgccgct ttcctccccg gccacggtga tgagcacctg taattcttcc
     2761 agcgtcaaag catttccccc tctcccccca gaaaggtcat cgggtctacc tcctccgtct
     2821 cctcccgatc agggtcatgc agacggttcc attcttcaaa aatcggcccg atctcgtcca
     2881 tgtagtagtc gttcagcagc tcccgcttgc tgatgccgat gctcagcgcc gcggcgatca
     2941 gcccctgaaa ccagtgttcg ctccgcccat gagagcctgt gcccgttttc ccagcgctcc
     3001 catggttttg ataaaatttt cgatcccgtt gacctccagc cacgcctcgg ccatttcacc
     3061 gaggccgtcc agcccgatgc gcgcgtcttc cagcagcgcc tgttcctcca cgccgcttag
//
LOCUS       DAWAEA010000153         3120 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4717_length_3120_cov_2.361501, whole genome shotgun sequence.
ACCESSION   DAWAEA010000153 DAWAEA010000000
VERSION     DAWAEA010000153.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4717_length_3120_cov_2.361501"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    212..333
                     /estimated_length=122
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    650..688
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1623..1632
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          528 a          952 c          835 g          634 t
ORIGIN      
        1 ctcgtattcc atgcccttca ggtcgatacc gggcatttcc gcgtcatact gcacttcctt
       61 gccgctgaaa acggaggaga ccagcgcctt gcccatgatg taatgtttgc cctccaccgt
      121 gatgcggcag taggtatcat ggggattgac gcacagcgcc aggttgctgg gcagcgtcca
      181 gggcgtggtg gtccatgcca gcaggtaagt gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncccttga ccttgaacag cacgaacgcg
      361 gaggtttcct tcacattctt gtagccctgc gccacttcgt ggctggacag ggcggtgccg
      421 cagcgggggc agtagggcgc gatcttgtgg cccagataca gcagaccctt ttcgtaaatt
      481 ttcttcaggc tccaccactc ggactcgata taatcgtcct tgtaggtgat atagggatgc
      541 tccatatccg cccagtagcc gacccggtca gacatttctt cccattcatg ctggtatttc
      601 cagacgcttt ccttgcactt cttgatgaac ggctcgatgc cgtaggcctn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnngt gcgcaataaa gggctccagg ccgtatttct
      721 cgatctgatc cttgccgtcc agacccagct gcttttccac ttccagctcc acaggcaggc
      781 cgtgggtgtc ccacccggcc ttgcgcagca catccttgcc cttcatggtc tggaaacgcg
      841 ggatcaggtc cttaatggcg cgggtctcaa tatggccgat atgggggcgg ccgttagccg
      901 taggcgggcc gtcaaagaac gtaaagcgtt ccgcgccgtc ccggcagtgg aagcactttt
      961 caatgatgcc cttttccttc cacaggcgga gcgtttcttt ttcgcgctcc acaaacttca
     1021 tgtccgttgg aaccttgcga tacatgttgg ggatgcctcc ttagtgaaga atggagaatg
     1081 attcccggct gaccgggagg atgcgggccg aacccataaa aaaagtccgt cccgaaaacg
     1141 ggacggagaa tgatggacat catacttccg cggtaccacc cgaattgacg gcgaatcgcc
     1201 gcccgctcct gtgtgctctg tatcgggagc tcccgggccg tcctcccctt tgtggctcga
     1261 gcggccagct ccaaggcgat gggcttctgc tgcctgccgt ccggcttgca ccctcccgga
     1321 ctcgctttgg cagaggaatg gcaaaagccg cccttgtcat tgcttcggat gtattatagc
     1381 gaaaaatcag aagaaagtaa agccccaaaa accttttgag cctcgttatc gggcgaaccg
     1441 ctcacttttt cttctttttg cggaagggcc agaccgccgc ccccgccgcc agcacgaagc
     1501 agacgacgga atagacggac aggtctccgg ccagcggcgg cgttgcgcgc agaagcgcgc
     1561 cggtcaaaag gagcccgcag ccaagcagcg ccccgcagac cttttccacc gagccccgca
     1621 ccnnnnnnnn nnatgatgct cgatgcccag attggcttcg cccttcaggc cggtatgaag
     1681 gatatctgcg atcaatgcgg ggatttccag cgatttgcga gtgctttcat acagcgcgcc
     1741 cgcgtcctgc gtcagctgac tgcgccagtc cacgtctgcc agcacccgcc gtcccaaccg
     1801 ggccgtgacc acgctcatca cgttcagctc cgggctcaag tcggccacaa cgccctcgat
     1861 ggtggccagt ccccgggcca gcatggtcag gctggagggc atggcgatgc cgttctggtg
     1921 catgacctcg gtcaagtcct gcatgacctc ggccagatcc atggtgccca ggtcggcgga
     1981 ggcgtacttt tccagcaaat cctccatatc ccggtacagc cggtgcttgt ccggcttctg
     2041 gcggaattcg cccagcccca gcaccgcgtc ccggcaggcg ttcacgtcgc cccgggccac
     2101 gccggtaatg gccttgccga tcagctcccg ctgccggtcg ctgagggagc ccatcatgcc
     2161 catgtccagc cagacgatct tgccctcccg cacccgcagg ttgccggggt gggggtcggc
     2221 atggaagaag ccgtcgtcca tgatctgctt gacgtaattg tccgccagct tggccccgat
     2281 ctcgctaagg tcgtagccgg cggcggccag ctcgtccttt tgggcgatgc ccacgccgtc
     2341 gatgcactcc atcaccagta cggtggtggt ggtgtagtcc cggtacaggg tgggcgacgt
     2401 gacaaaagcc acgtcggcgt tcagcttgtg gaaccgctcc agattggcgg cctcggtctg
     2461 gaaattcatc tcctcctggg ccaccaccca catttcgtcc aagacctgat tgaagtccac
     2521 cagttcgttc accggggcgt acttgagcag cttgcaggcc tgcttcagca gcaggatgtc
     2581 acggcccatg atctcgtgga tgccatcccg ctgaaccttc accaccactt tttcgccggt
     2641 ctgaagggta gcagcatggg cctgcgcaat ggacgcgctg ccaagcgcct gctgatcgaa
     2701 cgacgaaaag acctcttcaa gaggccttcc gtaagcgttt tcgatcaggg tcttcacctg
     2761 ttcgaagggc atgggcgcca cgtcgctgcg aaggcgggtc agcgcctcgc agtaagcctt
     2821 gggcagaata tccgaccgca tggaaagaat ctgccccagt ttgataaacg tcggccccag
     2881 ttcctcgatg atggcgcata gcttgtccgg cgtgataccc ttggtgatct cgtggcggcg
     2941 caccacctcc acgatctcca tcaggcgctg ccggttgtcg ctccgaccgt tattccgcgc
     3001 tgccatcctg ctcgtcctcg tctttcgctt cggtttcctc cgccgtcttc ccggcccgct
     3061 cccgggcggc gatgcgctcc ttcagcgcgg ccagctgttc ctcattcata ccgtccacag
//
LOCUS       DAWAEA010000154         3108 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4738_length_3108_cov_1.899116, whole genome shotgun sequence.
ACCESSION   DAWAEA010000154 DAWAEA010000000
VERSION     DAWAEA010000154.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4738_length_3108_cov_1.899116"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    297..385
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2199..2275
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          598 a          840 c          799 g          705 t
ORIGIN      
        1 ctcatgcctt tgtcctgata gcagaccagt tccttctggg ctttttccag cacgcttggg
       61 tcgatcgtag cggggcctgc ggcaaaattg tatactcgtt ccatcttcat ttcctcccaa
      121 cacccttgcg gggcaatgct ttctttctgc gtgggacgtt ttttgcccta tcggcgtttt
      181 ttcgcccttg cggatatatt acgcaggaga gccgtaaata gcaagttttt tctttgtatt
      241 ttttatgtgg aaatgagtat gaaaaaaggg acggttttcc gtcccttttc tagcaannnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnncttcc tttattcaat ttcttccccg cgctccaccc
      421 gggcgatcat ttccacccgg tcacggtggc gctgttcatc ggagaaatcc gtggtcagcc
      481 agatgcggag gatatccttc gccagctcgg tgcccagcac ccgggaaccc agggcgatca
      541 tattggcgtt attatgcagg cggctcatcc gagcgctgta ggtgtccgag caggtaacgc
      601 agcggatgcc gggcacttta ttggccgtga tgccgatgcc cacgcccgtg ccgcaaagga
      661 taatgccgcg gtcgcactct ccacttgcca ccgcacgggc agcccgcgcg ccgtaaaccg
      721 ggtaatccac gcttttgcgg tcattggtgc cgaaatcctt atattccaac cccagttcct
      781 tcaaaacctc gattgccgat tccttgagat cgaatccacc atggtcgcac gccagcgcta
      841 tcattcgact ttctcctcct gtgctccggg gctgcttccc cgtcttttcg aaaaatccga
      901 cttttatcat accactcagt gggtgaattg tacagagaaa gacgtgcaaa ctatcgacga
      961 ggtgaacctc atgatcgtca tggagaacgt ttgcaaatcc tatccgaaaa gcggcgggcg
     1021 gggcgcgatc cgcgtactgg acggcgtttc cttccacatc catccccggg aatacgcggc
     1081 ctttatcggc cccagcggca gcggcaaaag cacgctgctg aacctgatag gctgtctgga
     1141 ccttcccacc tccggccgtt acgcgctgga cggcgtagag gttccctccc tcggcgtccg
     1201 ggcgctttcc gccctgcgcc gggacaaaat cggctttgtt tttcagggct ttcagcttct
     1261 gcaaaagctg acggcctggg agaatgtggc ctttcccctg cttctgaagg gggtggagga
     1321 gccccgccgc cgggaggccg ccatggagac cctgcggagg gtgggcatgg cggaccgggc
     1381 ggagcatcgt ccctgccagc tcagcggcgg acagcagcag cgggtcgcca tcgcccgggc
     1441 gctttgcgcc tcgccccggc tgcttttatg cgacgaaccc actgccgcgc tggacccttc
     1501 cacccgggac gagctgcttt cgctctttgc ccgtctgcat caggaggggc acaccatcgt
     1561 cctcattacc cacgatctat ccgtggcccg gcgggccgag cggcgttttc gggtagacgg
     1621 cgggcgggta atagaggaag cttgatcgac ttcctttgcc cctttagact ggaaaaaaca
     1681 ggatttataa aaaacagaaa ccccgcaagc cttttctggc tgcggggttt ttcatggtac
     1741 gcccggtgcg attcgaacgc atgaccttca gagtcggagt ctgacactct atccagctga
     1801 gctacgggcg catatgggct cttgcgagca aaaggtatga taccacgaac tgaaggcgga
     1861 agaaagcgtc tgatatccca cccgggaagc cacctccgta atgggcagtt cggtggtttt
     1921 cagcaggttg atggcggcct tgacccgcac atggtacagg tattccaaag ggctggtgtg
     1981 catggtacgc tcaaattttc gtcggaagga cgtgcagctg atggcgcaca tgtcggccag
     2041 ctgctgcagg gtgatcggct cctgataatg gttggcaata tacagcaccg ctgtggatat
     2101 ctcattcatt aaggagggaa gtaccgtcag cggcgctctg ttttgcagca tccgccggag
     2161 catcacacat aattcgccca ccttgagccg caccagctnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcgac
     2281 agcccaagca gcattttctc caaatcaaca tccaaaaatt tccagccgct gatgctctcc
     2341 gggtcgcttt gggcgatatg caccacgttg ggcgctatga cggatacgtc tccccgctgg
     2401 tagcggtata cacgggtgcc gatgtaaaaa atgccgctgc cctgcaggca taccccaatt
     2461 tcggtggtca ggtggtaatg catatgatcc gccgggtggg gccgggaata atgcagccga
     2521 tccagataag gggcatgaat cgggaaatgc tcgtccaact ggatcggcat ggcacggata
     2581 gagtcctgta actttggaca aatctgcaaa gtttcatccc ctttttgcat tgaaaattca
     2641 ttttctgcca cttataatag cagacaaacg gactcttggc aagaacgtga agggggcctg
     2701 cttgtgaagt atcgttacca gctagggaaa aaacactatg agacagccca gacgaagacg
     2761 gctgccatct tgctgctgcc caccctgatc gcgctgacgt ttcttttttt gatcccggtg
     2821 gtgcggctgt tttctttgag cctgacctcc accaacacca tcaccaacca gtccacctat
     2881 atcgggctga aaaattacgc ctatctgctg aaaaacgcca tgttccaaaa ggcgtttagc
     2941 aatacgttgt ttttcaccgt taccaagctg gcgctggaga tcgttatttc tctggtgctg
     3001 gcggtcatgc tggatatgta cattccgtgg cgcaaatttc tgcgcatcag ctactttctg
     3061 ccggtgatcg tgccggtggt agcgtccagc attatctgga tgtggctg
//
LOCUS       DAWAEA010000155         3087 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4774_length_3087_cov_2.293536, whole genome shotgun sequence.
ACCESSION   DAWAEA010000155 DAWAEA010000000
VERSION     DAWAEA010000155.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4774_length_3087_cov_2.293536"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    400..607
                     /estimated_length=208
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1088..1310
                     /estimated_length=223
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1535..1544
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          563 a          801 c          787 g          495 t
ORIGIN      
        1 gtcaacgacc ttgtggcggc actgaaaaag gacggccacg acttctccgt gggcatcccc
       61 atggatacgc ccattcccaa ggcggagcgt gtggtctccg ccggtaaggg catcggcgaa
      121 aagaagaaca tgaagctggt ggagggcttg gccaaggccg ccggcgcggc gatcggctcg
      181 tcccgtcccg tggcggagac gctgaaatac ctgccccttg accgctatgt gggtatgtcc
      241 gggcagaagt tcaccggcaa cctctatatc gcctgcggca tctccggcgc cacccagcac
      301 ctcaagggca tcaaggatgc ctccaccatc gtggccatca acaaaaacgg caacgcaccc
      361 atcttcaaga actgcgacta cggcatcgtg ggcgacgtgn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnctt gccctctttg aaaactgctt tcccattccg cgcggcgtca gctacaacgc
      661 ctactgtctg ctggacgaga aaacggtgct gttcgacacc gtggactggt ccgcctgccg
      721 ccagctgctg gagaacctga accacgtcct ggcagggcgt gagctggact atctgctcat
      781 caaccacctg gagccggatc acgccgcctg catcgaggag atcctgctgc ggcaccccaa
      841 ggtgaaggtc atctccaacg aaaaggcctt catgctcatg cggcagttcg gcttccatgt
      901 ggacgaccat gagtgcatcg aggtgaagga gggggacacc ttctccttcg gccggcacac
      961 cgtcaccttt gtgggcgcgc ctatggtgca ttggccggag gccatggtga ccttcgacct
     1021 cactgacggc gtgctgttct ccgccgatgc ctttggcacc ttcggcgcgc tggacggcaa
     1081 gctgtttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gaggaaaagg
     1321 gcgtgctgat cgtctacgcc tccatgtacg gcaacacgga gaacgcggcg caggcacttg
     1381 cggcaaggct ctgcgacaag ggcatgagcc gtgtggcact gtacgatgtg tcctccaccc
     1441 acgtgtccca gctgatctcc gaggcgttca agtacagcca catcgtgctg gcctcggtga
     1501 cctacaatct gggcatctac cccgccatgc acgannnnnn nnnngcaaaa ccgcaccttc
     1561 gccctcattg aaaacggctc atgggcggtg aagtccggcg acctcatgca aaaattcgtc
     1621 aacaacgagc tgaagaacat gacggtgctc aacgagcggc tcagtctcgc ctccgcgctg
     1681 gatgaggaca aggccgttga gctggagacg ctggcggacg cgattttgga gtccgtgcag
     1741 tgaccggcgc ggggagcgga agaaagaaat aggctgccgg aaggctctgc cgcctccctg
     1801 ccggggagac cgtattccgc gagatggggc ggacaagcac gatcccgcgc ttgtccgccc
     1861 tttttccaaa agaggcacag cgagtcagaa cgtaacaaaa cgagtttcgg aaaggagatg
     1921 ctactatgaa ttacagtgct atgatccaaa accgcaagtc cgttcgcgcc tttcgcggaa
     1981 aggaggtgcc gaacgaggca ctcgcccagc ttcgcaccta ctatgaaaag acctgccccc
     2041 gccttgttcc ggagatcgcc acggagctga ttgtgctgga caaggacgcg cagccggcac
     2101 tggaaagctc cgcgggctac cagcagttcc tcatcggcgc gccgcactat ctgctcctta
     2161 tgtccgcccg gcacatccac gcgggcgtca acgccgggta catgatggag gatcttgtcc
     2221 tcaagctgac ggagctggac atcgacacct gctggctgac ctttaccgac agcgacaaga
     2281 tcaaaaaggc tctgtccctt accaccccgc tgcaggtggc ggccatcgtg gccttcggct
     2341 acggggaaaa gacggcgaaa aagctgcgga tgaacattca gagcatgtcg cagatcgatg
     2401 tgcaggccga gcagcagtac tacgcgccga aaaagagcgt gcatgagctg gtccacatgg
     2461 aaagctggag caacaagtcc gggcttgatg aaatgatgga cttctatgac gatatgctct
     2521 ggcaggcctt ctacgccgcc tccctgtccc ccagctacct caaccgccag ccctacggct
     2581 tccttgtccg cggccatagc atctatctgg tgcagcagga ggacgcctac accgacaacc
     2641 ttgacgccgc gctggatctg ggcatcgtgc tgctccactt ctccgccgtc gcctcccagt
     2701 gggcgggaag ggtgcgctgg gagctttccc cggatgcacc ggacggcctg cctgcagggc
     2761 atcgcgcggc ggccgtgtgc cgggtgtaga ggtgctgctt tcagccccta ttgggagcat
     2821 ttgcttcaga tagaaaaacc ggccaatgcc ggttcaaaac agaaaatcag gaggtgtttt
     2881 tccgatggcc atccgaacgg taacggatgc gatccgctat gtaggggcgg acgacaacac
     2941 attgacactc tttgaaaacc agtaccctgt ccagcctatg ggggtgagct acaactccta
     3001 tatcattttg gatgagaaga tcgctgtgat ggacagcgtg gacgcacggg cggcggagga
     3061 atggctttcc aatgtggcgc aggtgtt
//
LOCUS       DAWAEA010000156         3030 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4862_length_3030_cov_1.905546, whole genome shotgun sequence.
ACCESSION   DAWAEA010000156 DAWAEA010000000
VERSION     DAWAEA010000156.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4862_length_3030_cov_1.905546"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1740..1896
                     /estimated_length=157
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          609 a          848 c          917 g          499 t
ORIGIN      
        1 tacggctgtt actggacggc ggaatacaag ggccagtatg tttccatgct caccgaggaa
       61 gagctggctc aggccaaaac gctggtcatc tcccaccacg agggcctgat ggagcaggat
      121 ccagagcacg ccttcaccgt tcccctgtcc cggtaagacc gcaaaaaaat acaaaccaaa
      181 tgcacccgga gcgcataacg cgccccggcg caaagcggcg cggccctgaa ggcagcgggc
      241 agggagcccg cgaaaagaga aaggggtatt ctatatgaac gcttatactt cccgcgtttt
      301 ggaagggctg aagcagcgca acgccaacga aagcgaattt ttgcaggcgg ccacggaaat
      361 tctggaaagc atttcccccg tgtttgacaa gcatcccgag tacgaaaagg ccggtctgct
      421 ggagcggttc gtggagccgg agcgggtcat tcttttccgc atcccctggg tggacgatca
      481 gggcaaggtg caggtgaacc ggggctaccg ggtgcagttc aacagcgcca tcggccccta
      541 caagggcggt ctgcggttcc atcccagcgt caacctgtcc attctgaaat tcctcggctt
      601 tgagcaggtt ttgaagaaca gcctgaccgg cctgcccatc ggcggcggca agggcggcag
      661 cgactttgac cccaagggca agagcgaccg ggaggtcatg gccttctgcc agagcctgat
      721 gaccgagctg taccgccaca tcggcgcgga cacggacgtg cccgccggcg acatcggcgt
      781 gggtggccgg gagatcggtt atctctacgg ccagtacaag cgtctgaccg gcctgtacga
      841 gggcgtgctg accggcaagg gcctgtccta cggcggcagt ctggcccgca cgcaggccac
      901 cggctacggc ctgtgctact ttgtcaagaa tatgctggag gccaacggca agaccctgtc
      961 cggccagacc gtggtggtca gcggcagcgg caacgtggct atctacgcct gtgaaaaggc
     1021 cacccagatg ggcgcaaagg tcgtcgccat gagcgactcc aacggctacg tctacgacaa
     1081 ggacggcatc aagctggacg tggtcaagca gatcaaggaa gtggagcgcg gccgcatcag
     1141 ggaatacgcc gcccgcgttc ccggcgcgga atatcacgag ggctgccgtg gcatctggac
     1201 cattccctgc cacgtggccc tgccctgcgc cacccagaac gagctggacg cggacggcgc
     1261 gaaggctttg atcgccaacg gctgctttgc ggtgggcgag ggtgcgaaca tgccctccac
     1321 gctggaagcg accgaactgt tcctgcaaaa cggcgttctc tttgcccccg gcaaggcggc
     1381 caacgcgggc ggcgtggcca ccagcgcgct ggagatgagc cagaacagcg cccgtctgag
     1441 ctggacgttc gacgaagtgg acgagaagct gaagggcatc atggcaaaca tttttgccaa
     1501 gtgtgccgaa gccgcgaagg aatacggcga caagcccacg aactacgtca tcggcgcgaa
     1561 cattgccggt ttcctgaagg tagccgacgc catgatggcc caaggcaacg tctaaggcag
     1621 ggcaggggca gtacccctgc accccggact gcgcgccggt ggcgcgcagg gcgctcgagg
     1681 gaatgcagct tggtcggggt tttactgccg tggcacgacg agtgccacgg ctcgccggtn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngaaa aagtgtgaag caaagtcatg
     1921 ttttcaataa gattatcaga aaggaaagaa tgttaaaaaa cgggattgcg cccggaatgg
     1981 tttgtttata taatagttgc agaagaaagc gcgcctttga ccgggcggaa aggaacgttt
     2041 gaatgaagaa ggacaaggat cgttttgcgc cgtatcctcc catgggctgg aacagctggg
     2101 actgctacat gtccgccgtg acggaagcgg agctgctgga aaacgccctc tatcaggcgg
     2161 agcatctgct cccctacggc tgggaatacg ttacctgcga tattcaatgg tacgagccca
     2221 cggcggggac gcggcccggc tgcgaataca ttcccttcgc gccgctgtgt atggacggcc
     2281 gaccccagct ccatctgcct atggaacagc gatatgttcg gcacgcgaaa ggatcatccg
     2341 ggcgcgcagg cgtattacga cagtctcttt gaactatatg cctcctgggg cgtggatttt
     2401 gtgaaggtgg acgatattgc ccatactggc gaggccgaat ccggcggata cgcggcggcc
     2461 catgagatcg agatgatccg ccgggccatc gaccgctgcg gacgcgcgat cgtgctgagt
     2521 ctttctcccg gccccgcgtc cgtggaaaaa gcggggcacc tgagtcagaa cgccaatatg
     2581 tggcgcatca ccaacgattt ctgggacgac tgggccctgc tgaaaaatat gttcacccgc
     2641 tgcgagatct ggcaaagcca cgtcggtccc ggctgctggc cggactgcga catgctgccg
     2701 ctgggccgca tcggcgtaaa gttcggacag tcccggcaga cccgcttcac ccctgacgaa
     2761 caaaagaccc tgctgaccct gtggtcgatc ttccgttcgc ccctgattct gggggcggag
     2821 ctgcccagtc tggacgaagc cacccttcgt ctgctcacta accgggacgt gctgcggctg
     2881 aaccggcact ccttcggcgc gcggcagatc gtccgggacg acgcgcacgc cgtctgggtc
     2941 agccaggacg aagatggttc ccggtatgcg gcgctgttca acctgtccga aaagccgcgg
     3001 gaagtgtcgc tggatctccg ggagctggag
//
LOCUS       DAWAEA010000157         3026 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4869_length_3026_cov_2.280714, whole genome shotgun sequence.
ACCESSION   DAWAEA010000157 DAWAEA010000000
VERSION     DAWAEA010000157.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4869_length_3026_cov_2.280714"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    384..393
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          540 a          984 c          831 g          661 t
ORIGIN      
        1 aagtcccact ccgcgtcgga gtcggcgcgt caaaccccgc cgatgggctt ccttacggcc
       61 aaggcggcac atactgttgc ttacagtggg ctcccctcgg ggcaaacaat cggcgcaggc
      121 cgtactgacg tgcgctccgc ttgttgtttg cctgaactat tgggggatcc cccctgcccc
      181 attcccgcgc caatcagcac actttcttcc cccttccccc aaaaaataca atgattacgc
      241 cgtgttcctt tttccctttt gggataacct accattgccc cgccaccgcc ttttcacacc
      301 gggagaccct cccctccgct gccccgcccc cctttgcttt tttggttgcc tgtggtatag
      361 tatgcgggac gactcccgta ccgnnnnnnn nnncctgcgg cccgcgtgcc ggagcgtcga
      421 taagcctatc gaaattgttt caggaggacc tcttcgaatg aaagaaatcc tgttgaccct
      481 cgttaccgcc gtcgccgcct acctgctggg gtgtatctcc acaggcacca tcgtgtcccg
      541 ccgggagggc gtgaatatcc gcaatgaggg cagccataat accggggcca gcaacgtgct
      601 gcgggtactg ggcctgaaag acggcctcat tacctttctg ggcgacttcg ccaaagctgc
      661 cctcgcctgc ctgatcggca acctgctggt gccgtccgcc ttcggcatcg agggtcttgg
      721 acggatcgtg gcggcgctgt tcgtggtcat cggccataac tggcccgtgt tctatcattt
      781 tcagggcggc aagggcgtgg cctgctccac ggcggtggtg ctgctgatcc accctctgtg
      841 cggcctgccc tccatcctgc tgtgcgtgct ggtcattgct gtgaccaagt acatcagcct
      901 tggcagcatg accatgctgc tgacctatat ggtgctaatc tggatcgtgt gcttcggcca
      961 gtgggcgctg tgcatcttcg ccacgctttt gttcatcatg tgcgtatacc gtcaccgtgc
     1021 caatatccag cggctgctca atggcacgga aaacaagatc ggccgccgcg tccagcccca
     1081 gaacactgaa aaaaaataaa attcggctta tctattgaca aaatccgccg gtcatgctat
     1141 cctgccagac gacaactgaa tgcgtgtcct ttaaccacgg tcccgtgagg ccgggaaggc
     1201 tgcaaaagat atgccagtga atggtcgaaa tgtgtgtatc tgccttgccc tgccgtgggc
     1261 aaggcagttt ttcattttct accaaaggcg cgcagggttg atctttccga ctggaggaga
     1321 acgaaccgat gaaactcgtg tacgacgtca accagaggcc gcccctgaaa aagaaccttg
     1381 tgtatgcctt ccagcagctg ctggccatca tggccgccac cctgctggtg ccgatcctgg
     1441 ccgacgccac cggcctgtac ctgaaccagc ccgccgcgct gttcggcgcc ggaatgggca
     1501 ccctgttcta cattctggtg gctacccgcc gcaagagccc cgtgtttctg ggcagctcct
     1561 tcgccttcat cagcccgctg gcaagcgccg tggcgtatgg cttcggcggc atccttctgg
     1621 gctccgtgtt cgccggtctg gtgtatgtgc tgatcgctct catcattaag aagaccggct
     1681 ccgcatgggt caacaagctg ctgccccccg tggtcatcgg ccccaccgtg gccctgatcg
     1741 gtttgagcct gtgcggcagc gccgtgaaca acctgaacaa caccgccgcc ggcagctaca
     1801 atctggtttc cattctggtg ggtctggtgg ccttcttcat caccgtgtac gcctccgtga
     1861 agggccccaa gagcatgaga atggttccct tcatcatcgg cattctgggc gcttacgtca
     1921 tcgcgctggt gatgaccctg atcggcaacg ccaccggctg cgaagccctg cagctggttc
     1981 acctgtccgc cttcgacaac atgcagctgt tcaagctccc ccggttcacc ttcctgggca
     2041 tgttcggcgt gtacaaggac gctcccaaca ccatcaccag ctgggctgac gtgctgagcc
     2101 tgttcctgct gttcgccccc gtggccttcg ttacgctggc cgagcatatc gccgaccaca
     2161 agaacgtgtc cagcatcatc aaccgcgacc tcatcaccga ccccggcctt gaccgcaccc
     2221 tgatcggcga tggcgtaggc tccatcgtgg gcgccttctt cggcggctgc cccaacacca
     2281 cctacggcga atccgtgggc tgcattgcca tcacgggcaa cgcctccgtc agcactatcg
     2341 cgctggccgg tgtgtactgc atcctgctga gcttcttcga ccccttcgtc tgcttcgtca
     2401 actccatccc ctcctgcatc gtgggcggcg tgtgcatcgc gctgtacggc ttcatcgcgg
     2461 tcagcggcct gaagatgctg cagcctgtgg atctgggcga caatcggaac ctgttcgtgg
     2521 tcgctgccat tctggtgtgc ggcatcggcg gcctgacgct gaacttcggc tccgtgcaga
     2581 tcagctccat cgccaccggc ctgatcgtgg gcatcctggt gaacgtgatc ttcaacaagc
     2641 gctctgccga ggaaaagggc gaagccaagg ccgaataaga aaagccttga cgcaactgca
     2701 actttggggt atgtttctga caaaggaaca tacccctttt ccaaaccgaa aaggcttctg
     2761 tttaggagga ctacgccatg tcagagcagc agctcaaagc caaactgatg gacgcgcccg
     2821 ccatgcagcg ggccctgagc cggatggctc acgaaattct ggagcagctc gatcccccgg
     2881 aaaacatcgt gctggtgggc atcaagcgcc ggggcgtgcc gctggcccag cggctggccg
     2941 cgctgatcga gcgtttcgag ggtgtacggg tgcccgtggg cgatctggat atcaccctct
     3001 accgggacga cctgactacc ctatac
//
LOCUS       DAWAEA010000158         3009 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4902_length_3009_cov_1.937373, whole genome shotgun sequence.
ACCESSION   DAWAEA010000158 DAWAEA010000000
VERSION     DAWAEA010000158.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4902_length_3009_cov_1.937373"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          607 a          899 c          998 g          505 t
ORIGIN      
        1 atgtccaaga gcctgggcaa cggcgtggac ccgctggaga tcatcgacaa gtacggcgcg
       61 gacgcgctgc gcttctcgct ggccatgggc gtcagccccg gaaacgacac ccgctattcc
      121 gacgagaagg tggaaagcgc ccggaacttc gccaacaagg tctggaacgc ctcccgcttc
      181 gtgctgatga atacccagac ccgggagacc atcgacccgg ccaggctgct tctgcccgac
      241 cagtggatcc tgtcccggct gaaccgcacc atcgccgagg tgtccgccca catggaggac
      301 ggcgatttcg gcatcgcggc ccagaatata tacgacttcg cgtggagcga gttctgcgac
      361 tggtatatcg agctgagcaa gggccggctg atgggcgagg acgaggagca gaagcagaac
      421 gtacgcgcgg tgctgctgta tgtgctggaa ggtctgctga aactgctgca tcccttcatg
      481 cccttcctga ccgaggaagt gtaccagtac ctgccggagc atgacggcat gctgatcctg
      541 gctcgttggc ccgaggtcaa gcccgaatac gacttcccca aggaagcccg gcagatggag
      601 ggcctgatgg aggtcatccg ctccgtgcgc aacctgcggg cggagatgaa cgtgcaggcg
      661 ggcaagcgca cccatatcgt cctgatccct gaagagggct gggaggagac gctggccatg
      721 gccgagccct atctgcagcg catggcctat gccagcaccg tagaactggg aggcaaggag
      781 gcactggccg gtcagaaggt ggtcagcgcc gtgtgcgccg caggcgagat ccgcattccg
      841 ctgggcgatc tggtggacat tgacaaggaa atcgcccgtc tggaaaagga acagaagaac
      901 ctgcagggcg aaattggccg ggccagcggc aagctgaaca accccggctt cctgaacaag
      961 gcccccgccc atctggtgga gcaggagaag gaaaagctga aggtaaacga aaccaagctg
     1021 caggcgctgg aggcccgcat tgccgacctg aaggcgggtg cctgatggac gtgctgctgg
     1081 ggaccaccaa tccctctaaa gcggctatgt ttgaaaacgg gctggcgggc gaggacattc
     1141 gctttctgac cctgcgggag gtttccattc cgggcgaacc ggaggaaaac ggcgcgaccc
     1201 cggcagaaaa cgcccgccgg aaggcggagt actacgggcg gtacgccgat tatgtgatct
     1261 gcaacgattc cgggctgtat ctggccgggc ttcccatgga ggacgcccgg ccgcccggcc
     1321 tgcacgtgcg cacgccgggc ggcgcggcgc accggctgga cgacgacgag atgatcgcct
     1381 attacgccgg attggcacat tcgctgggcg gatgggcgca ggcgtgctat ctgagcggct
     1441 acgcggtgaa aacgcccgaa aaggtgtttg tttatcagga gcggatggaa gatcggacgc
     1501 gcgatctttt ttggttgacg gatacgccct gcgtggaacg gcaccccggc tggccgctgg
     1561 attccctgtc ctattacagc gaccgaaaaa catctttcct ttcgcccaag ccgccggtac
     1621 tatccggccc cgaggacgat cgggaatgga aacgccttct ggcgtttctg aaagaatctc
     1681 tggggctgtc gtcccggagc cgataaggag tacccatgga atttcaccat attccggttt
     1741 tatttgacga agtgatggag tggatgcggc cccgtgccga cggcgtttcc gccgacggca
     1801 cgctgggcgg cggcggccac agcgagggta tactgcgcct gtccggcggc acggccacac
     1861 tgtacggcat cgaccgggac gaggacgcga tccgcgccgc cagcgaacgg ctgaaaatct
     1921 acccgggctt tcacgccatt cacggcaact tccacgacgc aaaagcgctg ctgaccgccg
     1981 cgggggcgcc gcccctgacc ggggctttgc tggacttggg cgtcagcagc caccagctgg
     2041 acgagggcga ccggggtttc agctatcacg aggatgcgcc gctggatatg cgcatggatc
     2101 agagtaccgg cctgacggcg gcggaatatc tggcacaggt cacgccggag gaatttcgcc
     2161 gcattctgac cgactacggc gaggaaaaat gggcggcccg tattgcacaa atttttgcgg
     2221 aaaagcgggc gcagaaaccc attgagacca cggccgatct ggtggcggtg gtggatgcgg
     2281 ccattcccaa ggccgtgcgc cggaaggacg acggccaccc ggcccggcgg acgtttcagg
     2341 cggtgcgtat cgccgtgaac gacgagctgg ctccgctgga aaacgcgctg cgggattttg
     2401 tggagctgct ggctcccggc gggcggctgc tggtcattac cttccattcg ctggaagacc
     2461 gcatcgtcaa gaataccttc cgggccatgc agcatccctg cacctgtccg cccaaagcgc
     2521 ccatctgcac ctgcggcaag gttccgctgg gcaaagtgct gggaggcggc ggggtcaagc
     2581 cctccaatca tgaagtgcag gagaatgtcc gggcccgcag cgccaagctg cgcattttcg
     2641 aaaaaaacag ggaagagggg gagtaaagga tgccgacgct ctatgtggtg gctacgccca
     2701 tcggcaattt gggggacatg agcccccggg ccattgaaac cgggcacccc ctgcatcagc
     2761 gaccccggca gcgtccttgt gcgggaagcg gcggagcggg gcattcctgt agtggcggtt
     2821 tccggcccca cggccatggc ctcggcgctc agcgtcagcg gcatggacat ttcggaattt
     2881 accttctacg gctttctgcc ccggcaaaag aacgaactgc gggagaagct gaccgacatg
     2941 gcctggcatt cgaagatcgc cgtggtgcac gaatccccgc accgggtcat cgcgcttttg
     3001 cagcaggta
//
LOCUS       DAWAEA010000159         3003 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4913_length_3003_cov_1.863636, whole genome shotgun sequence.
ACCESSION   DAWAEA010000159 DAWAEA010000000
VERSION     DAWAEA010000159.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4913_length_3003_cov_1.863636"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          572 a          978 c          846 g          607 t
ORIGIN      
        1 ctccacatca tcccacttca gcatcctgtt cataggcggg cggcagttgg ttcgatattc
       61 gaatcggctg tttctccagc gccgcctcgt acagataggg cgcgtccgtt tccggcaaag
      121 agcctgtcca catgcggtgc aggatcgccc caaaggcaaa ggtgtcgatg gccgggccca
      181 cgtctgcctg ctctcctgcc atgcgcagaa aggtctccgg ggccagatac accgggtcgc
      241 cctctgtgtc gccgccgggt gcgggcggat cgtccgccag aaagccgctg tccagatcga
      301 tcagccgggc ttcgtagccc agttccccct tacggagaag cacatgctca ggcttcagat
      361 ccgcgtgaac gatcccctgc agatgaagcc gctgcagaca cagcgccaga ctgagcaaaa
      421 tttcccgctt gtcctccatg ggcagcgtct caacggcgct ttcccccgtc agacggggtt
      481 cgggaatctc ctccgaaacc gcgtaatagt gcagcccgaa gcggaagaag tccgttaccg
      541 gcaccaaggt gtcgccgatc acgcaggaca tggcgctgta caggcgtcgc tttttttcct
      601 cgaaacgacg gcagcgggcg agctgctttt cccgcaggac ggggttggcg ttctccgccg
      661 ggtagaccgg ggaaagaaat tccttcagaa aaaagcgccg ctgtcccctc cgggcgatgc
      721 accagcgggc actgccgctc cccgccgtct gcagcggggt cagcagctca taggctccaa
      781 tctttttcat gctgaacttc ctcggttcgg tcatagccgc gctgatagcg tttccagctt
      841 tcccgcagcg cgtccgcgtc cccggaatgc tgccgcaagc tgttcagatg ctcctgccac
      901 agcgccgtca ggcggggttc aaaataccgc ttcatttcgt caaaatggtc aaatccgaag
      961 ggcagacagc agagagtcgt gtcgtccccg gccagacggc ggatggccgc cgccagacgg
     1021 cgttgccagg tagccgcatc ctgcgcgccg gtcagcgaag ccagaaagat ctcctccagc
     1081 tccatggggc taaacacgca gccgtatacc ccgtccgtgg cacagatcag cagccccttc
     1141 ggcgggagtg taaccgaacg cagatgaagc cgcaccggcc ggtcggcgct gacgcacagg
     1201 ctcaacggcg cgtcgcagcg cagacgttcc agctcgtcca tgcggaattt cacatcgtcc
     1261 cgggtataca ggtgcagccc gtcctcatcc agcacatagc cccggctgtc ccccgcccac
     1321 agaaagcagg cccgttctcc catatcgtac agcgccatgc acagcgtggt aggcaaaagc
     1381 cgctgcatac tgcccacgat gcggctgctg ccctctgtga gccggtgctc ctgcacaaag
     1441 gcgttcaggt cggtttccag cgtttcctga ccctctgccg gtgttttcgg gagacaacgg
     1501 caggcagcgg cccaacgctt cacgctctgc gtcgccacgc gggaggccgc gtatgcgccg
     1561 gtctgaccgt ccagctccgc atagcgtcgg ctgcccagcc cgccgcagcc gtcggccacg
     1621 cacaaaaggg acaccggcga tttggataaa atcgaatagc tgtcctcccc ctgccggggc
     1681 agaacgccgt gattcaacgt taaaatacgg gaagaaatac aaagtccccc ttaaatcgtg
     1741 ttgcagacgg agtccagcac ctgcgaatac tccacgtcct ccaagccctc gctgaccgtc
     1801 tgcacattga tcacctgtcc ccactcggaa gaaatgcggt tccattccgg cgcgtccggc
     1861 gcaaagatca gcaggcgttt ggcattttca tccatgcagc cgcccagctg ctcatcctcc
     1921 caccagaggg tcagctcgtc gaagtcctgc gccatgccct ccgggtacca cggggcgatc
     1981 ttgccatggc ccagttcatg ggtgggcgcg tccgaccaga cgacgataat atgccgcttc
     2041 ttgaccccct cccgggtcca gtcgctgcga atggcgtagg ccagagcttc cagcccgtcc
     2101 tccggaatat cgccgccgcc cttggcatgg atgccttcca cgcagtcccg cagcatttcc
     2161 gcctgctccg gcagggtgaa aaaatcgctg gacagcatag cttcctcgcc gtcggcaata
     2221 tagtcccgga aggcgatcac cttcacccgc agctggtgga tggtcttgtg ctttttctcc
     2281 atttcccggg tgatatcctg atacagattc agctcgttct gtttcacaaa atccagcaca
     2341 tggcgcatac tgccggtgga atcgatgcaa aataccatat cgacgttgta agtaagctga
     2401 taccccgcca cggttcgtcc tcctttgagc tcgtaatgaa gaatattgtc gtatcatggt
     2461 aacattctgc gcaaagcggc ggatttcctg ccgtttttca gaaggaaaac cgtgccgggc
     2521 agcgaaatga agtaggtgga ttgattttgg aaaggactgg agctttcatg aaattccgga
     2581 agacgctggg cgttctatta acccttttgg gctgtatcgc tgcttttctg gggctggcgg
     2641 cggcggtcgt gccgctggtc gccaacgcgc agctgcagtt ggtgctggac tcctttgcca
     2701 cgccctcccg gagtctgttg gtcaacgcga tcaaccaact gatgactttt gttctctctc
     2761 attccctgcc ggttctgctg gggggactgc tggcgcggcg gttcttttca ggcgattcgg
     2821 gaggaacggg ccaatgactt cgccccggcg gcgcaaagtc atgccgcttc attcgccagt
     2881 gtgaaatcca cggagcgcag cccctatcaa cggcccgaag cgcaggagcg cagtccctac
     2941 cagcgcccgg aggagccggt tgttcaaccc ttcgatcgtt cggacgattc ctttcccgcg
     3001 ccc
//
LOCUS       DAWAEA010000160         3000 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4918_length_3000_cov_2.076401, whole genome shotgun sequence.
ACCESSION   DAWAEA010000160 DAWAEA010000000
VERSION     DAWAEA010000160.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4918_length_3000_cov_2.076401"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    422..431
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2118..2127
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          556 a          862 c         1011 g          551 t
ORIGIN      
        1 ccgccatgcc ccattatggg gacagctgca ttaccctgcg ttacccggag gacacgtccg
       61 tccgcagcgt cagcccgctg ctgaacgctt tgctgggcga aggggcggac ggctggcttg
      121 cagaaccgtt ttcccagggg cttttgatgg cggaggggga aacaactccc tttgccccgg
      181 aaatgacccc ctatcttcgg gtagaaggag ccaagaaaac cacgtggttt gctccgcccc
      241 tttccccgtc gctggcccga tggctggaag aagccggcga acgccccgga gagcacgagc
      301 cccatttgct gccggaaagt ctgtgctgcc gcctcctgcc ggaaggggtg gcggaggccc
      361 gctttgcgct gggctgccgc gtggacgcgc ccgaagggat gcgccggggg cagatcacct
      421 tnnnnnnnnn nccgctaccg cctgtgcgcc ctgccggaag aaggcggcgc gtctgcgctg
      481 gacgctgcgg ctttgccctc cgtcacggtc tggcccaacg tgcgcctgaa cggcgggcag
      541 tggcggcagt actttgtcca cacccatcag ccggaaaatc tggacgtatg gtcgctttcg
      601 caggagggtt gggtgcaggg cccatggaaa acagcggaga accgccgctg gcagaccgcc
      661 tgtgtggaac gtttcccggc catgatcgtg ctcaaacggg gcgggatcag tctgggcgcg
      721 ctggtcaacg accggcccgt taccctgatc cgccacgagg aagaagcagc cgccgggttg
      781 gatttcggca gtatcgccac cacggtgatg ctgcgtcttg gcgaaaagac ccagcccgcc
      841 gcgctgccgg aaggactgta cgggcggctg ctgggccatt cggaagaagc atggctgacg
      901 gacgaatttc tgtcggagca gccggacgca agcacgtgct acagcgtgat ggagatgttt
      961 ggagacgaac ccgaaaaatg gcggggcgta ttgcaggacg ggcacattta cgcgccccgg
     1021 agcctgacgg cgctgctgaa caagcccagt cgcagcctgt attacgacct gaaatggagc
     1081 gacgagaact acgccctgcg ctgtctgcgg ctgtttttga aacaggtcat gctgcaaacg
     1141 tctctggcgg cgcttttgag cggcgggcct gccttaagct ggcgagtcag tatgcccggc
     1201 gcgctgccgc cctatcgtca ggaagcttat ctggaaatgg tgcgggggct tgcccgggag
     1261 gtggccaagg aaacgggaat gccgctgagc gctcggttcc cggcggtgct gtacgcgctg
     1321 gaaaatcagg cggacggctg gtatttcctt tcccgcagcg aggtggacgc ccggggcggg
     1381 tatctcaatc tggatattgg cggctccacg gcggacttga gcctgtggct gggcggaaag
     1441 aaccacgcgg cggcggaaag ctccctgctg ttgggctgcc gccagatgct ctatacctcc
     1501 tttgcgcggg ggcacggggc ggagtttgcg gcggatttcg cccaaactcc cggctccatg
     1561 gccgaagccg ttgcaatcat cgcggaccgg ctggatcacc cgggatcctt gggacgggaa
     1621 gcccaaaaga ccatgctgct gatggacgat ttctttgcgg cctatgcgcc ggaaattcgt
     1681 caggccatgg aagaagcccg cgccgggggc agggtcagct acacggaatg tctgctgctg
     1741 ttccagtttg ggttcctgtt ctatctggcc gggctgatga tggagcgggc gtggcaggag
     1801 ccggagctgc agcccctgat gccggtgaag ctggtggtct gcatcgccgg gaacggcggg
     1861 cagctgatga agaccctttc tagggaacag gcggacggct tgtgccgcat ggcgctggaa
     1921 gccttgagcc gggagcatcc caccgaagca ctgatgccgg tgcagagcga cgcgcccaag
     1981 cgggaggcgg cgctggggct tctgtatgaa gacggcgact tccaaagcac actgcagagt
     2041 cgggaacgct ggaatgggac ctttaccgcg tccggccagc cggaagaaaa ccggctgacg
     2101 gcctatctgc tgcgcttnnn nnnnnnnggc tccgtccgcc cggatggaac tggaaaccat
     2161 ctgggccaac gaacgggcca aacacccgga ggacgacttc gccgcgtggg tcggtagcct
     2221 gacggccttg aaacagctat ggaaaatttg acggaaagaa ggaaaaacga tgctgtattt
     2281 tgaaaacgat tattgcgaag gagcccatcc ggccatcctt cggcggttga tggagaccaa
     2341 tctggaaaag cagtccggct acggccatga cgcttactgc gacagcgcaa aggaaaaaat
     2401 tctgtcagtc tgcggctgtc cggaggggga cgtgtacttt ctggtgggcg gcacccagac
     2461 caacgctacg gtgatcgcct cgctgctgcg ccgctatgag ggtgtgatcg ccgccgctac
     2521 ggggcatatt gccgctcatg aggcgggagc tatcgagttc accggccaca aggtactgac
     2581 cctgccccag catgacggca agctggacgc gggggaagtg gagcaatatc tggtagacta
     2641 ttatcatgac ggcagctatg accatatggt gttccccggc atggtgtaca tctcccatcc
     2701 aacggaatat gggacgctgt attccctgcg ggagctggaa gccctgtccg acgtttgcgc
     2761 ccggtatcat ctgccgctgt ttctggacgg ggcccggctg ggctatgggc tggtcagtcc
     2821 cggaacggac gtgacccttc gggatattgc ccggctgacc acggtgtttt atatcggcgg
     2881 caccaaagtg ggcgctcttt gcggcgaagc cgtggtgttc ccgaaaggtg cgccggagca
     2941 tttcttcacc atggtcaagc agcagggcgc tctgctggcc aagggccggc tgctgggcat
//
LOCUS       DAWAEA010000161         2993 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4934_length_2993_cov_2.520422, whole genome shotgun sequence.
ACCESSION   DAWAEA010000161 DAWAEA010000000
VERSION     DAWAEA010000161.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4934_length_2993_cov_2.520422"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    842..851
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          595 a          922 c          956 g          510 t
ORIGIN      
        1 ctgctgggca gcgaggtcat ggaaattcac gcgccgctgg cgattttcgg caacggcggc
       61 accatttccg aggtcagcta cagcgtcaac acccgcgtct atgcgggcac gaccctcttt
      121 acgctggaaa acgagccgga taccgacaac taccgcaagg ccctgtccga ccgcaacgaa
      181 aaggccgggc agctgcagga catgctgatg ctccagacca gccgcaacgt ggtcgccccg
      241 gcggacggca cggtgcagct gatcggcgtg gaggaaggca aaaagaccgc ctctgccgac
      301 ggctccggcg atgtgaccgc ctttacgctg ggcagcggcg gcgcggtcaa gatgaccgtg
      361 gaggtggatg agctggacgt ggccaaggtg caggtgggtc agagcgcgga ggtcacgctg
      421 gacgctttct ccggggagaa attagccgcc gaggtcagcc gcatttcccg catcggcaag
      481 ccctccggca gtatcaccgt gtatgagacg gacctgatgc tgaccgggga tgaccgtctg
      541 ctggagggca tgaacggcac cgccgtgatt ctgtccgaac ggaaggaaaa cgtgctgctg
      601 atcccgctgg gagccgtcca cgaggacgaa aacggctcct atgtgaaccg ggtcactgcg
      661 gacggctcca cggagcgaac cgccattgaa acgagcctgt ccgacggcag caacgcggaa
      721 gtcctttccg gcctgaacga gggcgacgag atcgcctaca cccccgcctc cacccggaga
      781 atgttcggct tcggccccat gagcggggcg ggcttcggag gggtgcagcc atgacggaaa
      841 annnnnnnnn ncggcgagga agtccgggcg ctggaccacg ccaccctgac catccgggaa
      901 ggggagttcg tggccattat cggccccagc ggcagcggca agtcgacgct gatgaacatc
      961 atgggctgtc tggacaccgc cgacagcggc gaatacctgc tggacggtca gcagatcgag
     1021 cactattccg aggacgagct ggcccgtatc cgcaatcgga aaatcggctt cgtatttcag
     1081 agctttaatc tgctgcccaa gatgaccgcc caggaaaacg tggagctgcc tttgatctat
     1141 cagggcgtga gcgcggcgga gcggcggaag cgctcggccg aagtgctgcg ccgggtgggg
     1201 ctggaaagcc gcgccgggca caagccaacg gagctttccg gcggccagca gcagcgggtg
     1261 gccgtggccc gggcgctggc gacggagccg gcgctgatcc tggccgacga gcccaccggc
     1321 aatctggact ccaagaccgg caaagagatc atgagcctgt tctgccagct gaacgaagcg
     1381 ggcaagacca ttattctcat tacccacagc gaagccgtgg cccagcaggc ccaccggcgc
     1441 atccgcatca tggacggcca tgtgcgggac tgcggcgacg agaccgaccc cttcggcact
     1501 gccgaagtat cccccgaaaa ggcaggtgag gacgcatgag catccaatgc ctgaaaatgg
     1561 cgtgggaatc catcaagagc aacaaaatgc gctcctttct gaccatgctg ggcatcatca
     1621 tcggcgtgac cgcgctggtg acgctggtct cgctggtgaa tggcgccaca ggagccatca
     1681 ccagtcaggt ggaggcgctg ggcaacgaca tgctgatggt cacggtcatg gatgataaat
     1741 ccgcgcccct gcggctggaa aatctggagg agatcagcag gctggacggc gtgcggcaag
     1801 cgtcccccag cggctctatg agcgcccggg ccaagcgcgg ctccactgag acctccgtca
     1861 gcgtctacgg cgtgttgccc gcctacgcgg acattcaggg tctggagctg gatcggggcc
     1921 gcttcctgaa aacggcggat gtggacaacc gctcctatgt ggcggtgctg tcccacgatc
     1981 tgcacgagac cctcttcggg gacgaggacg ggctggatca gttcatcacc gtcggcgggc
     2041 gcagcttccg ggtggtgggc gtgctgaagg aaagcgactc catgatggtc tccatgatga
     2101 acagtggcac gctgtatgtg cccttcacgg tggaaagccg catggcgggt cagccggaca
     2161 tcatgtcctt ctacgtttcc tcctccggca gcaccgacga cgcggagact gccgtcaacc
     2221 agtatctgct gcggcgcttc cagcaggatg aggactcttt cagcatcctg aatatgtcca
     2281 gcgtttcctc ggccatggat accatcaccg gcaccctgtc cctgctgctg ggcggcattg
     2341 cggccatttc cctgctggtg ggcggcatcg gcgttatgaa tatcatgatg gtctccgtca
     2401 ccgagcgtac ccgggagatc ggcatccgca aggccatcgg ggccagccgg cgcagcatca
     2461 tggcccagtt cctgatcgag gccctgctca tcagcctgct agggtgcttc ttcgggctgg
     2521 cgctttccgg cggcatactg gccattgcca ccgcgctgaa cagcaccatc gttttcagca
     2581 tgtcgccgtc ggtggtgatc ctggcggtgg tgttttcctc cggggtgggt ctcattttcg
     2641 gcctgtaccc ggccaacaag gcggccaaga aacaccccat cgaggccctg cgctacgaag
     2701 ggtaagagtc aaacgggacg cggcggaacg ctgcgccccg ttttttctgt cagccgggaa
     2761 gggaaacgga ggaagcccgg cgggaggaaa gtccaagtaa attcaccctc tgaccgcccg
     2821 gcacaagtcc catccactga ccgtaaagcc ctcctaaaaa cttgccgcgg cttctcaacg
     2881 attcccccaa ccccttccca tctttcaggc agaacgtctt tcggcgctct gctcagtatg
     2941 tcaaaaaagt ggaggtgcag gaggcactgc tgccactcaa tcgtcgcagg gac
//
LOCUS       DAWAEA010000162         2971 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4990_length_2971_cov_1.541495, whole genome shotgun sequence.
ACCESSION   DAWAEA010000162 DAWAEA010000000
VERSION     DAWAEA010000162.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4990_length_2971_cov_1.541495"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1090..1099
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1648..1657
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2399..2408
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          518 a          870 c          887 g          666 t
ORIGIN      
        1 gttttctgac cgtgcgctgg gtcacatatc aggcgggcgg agcgatcata agcgagctta
       61 catccttccc tatccccttg tcgaaggaat ttcagcagaa gctggagacc gagcctttta
      121 accagctgat gtcccttggc tatagcagcg aatttagcac ggacgatttt cttgacccgt
      181 ctttgctgcc cgtttcgggg aaaatcctcc aaaatcatct ccagtccgaa gttctgcggc
      241 tcagctggct gacctgctac ggggaaaacg aggatttgaa ctgttccttc gggctgaaca
      301 ccggcacttt gatggatacc gataccctga ggatcggtgt gctgcccttc gacctgtttg
      361 cggacgatct gaccacattg ccctgcacct ccgaggagct gaccgcccag ctggaccgga
      421 ccgggctggc ggtggtatgc aaccccgatc ccaacgaccg ccttcacctg cggacaaagc
      481 ccagccggga agcgaattcg ctgggcaagt tctggaacgg cacgcctgtg cgggtgctca
      541 atgagcggga cggctggtgc caggtggaga tcggcacaga cgggcggctt ataggctgga
      601 tgatgaaaaa gtatctagtc accggcgcaa agatggatca ggtcacaccc cgcttttccc
      661 aacagacctt gcgggacgat aaggcggaaa ccgaaacgcc catctatacc gacctaagcc
      721 tgaaggagcg ctattgcacc cactccggct gggagctgat gggcgtggcg gacgaccggc
      781 tgtacgtcgt cgtcaccgac gagggcgaga caggctatgc gcccatggag tggttttttg
      841 acggaaacgg ctgacggagc cggcccacca gacgggtggc gcggggaaaa cccgtgccgc
      901 tcttgacttt ccgggcgctc ttttctataa tcagggtggt ttgccactca tgcggcaaaa
      961 acgcgcaaag gaggctgcac ggcttgacgc atcgcaacag cgtccgaagc agctggcttt
     1021 ggggcgcagg cattacggcg ctggcgtggg gctttttgct ctgcttcagc gacatttact
     1081 ttgctgccan nnnnnnnnnt tcatcctgcg cgccatgacg ggctttcagc cgggcggcac
     1141 gcctgatttt cacccgtttc tgctgggctt ttatgtttat cccctgcgct ggctacagcg
     1201 cttttttccg ggcgtcgcat ggtattccct tttggagctc cttctgctgg cgctggccct
     1261 gaccgtgatt ttgaaaagcc tgctgcaatg ctggctccgg gcgggccgac ggctgcggac
     1321 aggtcttctg ctgtgcgccg gttatgccgc tttgtatggg tcgaactatc tggctcgggt
     1381 tacctttacc gtcacgggag cgatgctggg ggccgctgcg gtggcgcaga ttttgtccat
     1441 cgactgtcaa agcgcctccg accgttccct ccttcgggcc atgacgctgg cgatactgct
     1501 gctcattttg tgctacggac tgcgccagct ttcgctcgta ccggtgctgg gctattgcgg
     1561 gattgctttt ttgctgcgct tttgttcatt tttcgggttt ggaaaacaaa cgaagcgctc
     1621 cgcccggccc atgttggttg cggcgggnnn nnnnnnnacg cgcttcagtg gcagcaggcc
     1681 cgcagtcagg tgctggatta tatcaatctg aaatccctgc cgtccgaggc ccttgaaagc
     1741 gtcggctgga cggacgagca gcgcatcctg ctggacaaat ggaacaccat ggatgaggcc
     1801 atctccacgg aggccctccg ttctgtgcgg aaaaactggt acaattccga aacgaccacc
     1861 accgccggag ccgccattga ggaccttcgg tggcgctcgc cgtggttcgt tcagaccatg
     1921 gtcgtgctgt tcgggctggg gctgtttgcc ctcttctgcg ccttccggaa ccgtcgggag
     1981 aatccatggc ttccgctggc gctgcttatg acgggacttc tgtgctttct gtttttctgc
     2041 tatctggcgt tgaagggccg tctcccctac cgagcagtga cggtggccct actgcctgcg
     2101 gcggcgatgg tcttctgtct gttgggagaa tgtcccctgc ctacggcccg gaagggctgg
     2161 agggccgcgc tgggcattct gctggcggta agcatggccg cgccgctgcc agccctgctg
     2221 ggcgctgtcc ggcggcgtcg ctccccgtgg gactacaacg cccacgcgga tatggacgcg
     2281 caggcgctga cccacccgga tcttttgctg atctactcca ccgagctggt gaacgacatg
     2341 cgcatgttcc ccgacacgtc tcagggcgta ccgcaaaacc tgaccttctg gggcggatnn
     2401 nnnnnnnnct ggctgcggcc cgaacttcgc ttcatcagcc tgaaggaagc gccggacgaa
     2461 gcgctgctgg cctacctgcg ctccgagctg gggccggtgg aatgggaggc ggagaaggtt
     2521 tccgccgggc tgtatttctt ccgctttttc cagccctgac gtactgaaaa aacaggcttt
     2581 atcaagcttc atccatcttc atcagaaagg ctggttttcc atgaaaaaac tgtttcagga
     2641 cttcaaaaaa ttctgctccc gcggtaacat tctggagctg gccaccggcg tgatgatcgg
     2701 cggcgcattc accgccatcg tcaactcgct ggtgaatgat atgctgatgc ccgtcatcgg
     2761 cctgctgact ggcggtgtga acctgagcgg cctattcttc gcactggact tccagagcta
     2821 ccccagcgcg gaagccgcca acgctgccgg ggtgggcacg gtgaactacg gcgctttcct
     2881 gcagaacatc ataaatttca tcatcatcgc cttctgcgta ttcctgctgg tgaaggccat
     2941 gaccggcggc ggcccggacg gctcgtcgga a
//
LOCUS       DAWAEA010000163         2968 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_4998_length_2968_cov_2.072434, whole genome shotgun sequence.
ACCESSION   DAWAEA010000163 DAWAEA010000000
VERSION     DAWAEA010000163.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4998_length_2968_cov_2.072434"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          625 a          895 c          928 g          520 t
ORIGIN      
        1 gctttggccc cagcctgctt gcggccattc ataaggagtt cgacctgcac tgcgacgtgc
       61 atctgatgat ctccgacccg ctgaaatatg tggaaaactt cgccaaatcc ggcgcgggca
      121 ccatcaccat ccatcaggag gccaatgatt tcctgccctg cctgcggaag ctgcgggagc
      181 tgggcgttcg ggtgggcgcg tcggtgaagc cgggcacctc cgtcgaaacc ttgcgggagg
      241 cgctgccgct gctggatcgg gtgcttctga tgacggtgga gccgggcttc ggcgggcaga
      301 agctgatgcc ggatgtgctg cccaaggccg cccagctgcg ggctatgggc tttaccgggg
      361 atatcgaggc cgacggcggc atcaatgggg acaacgcctc ccagctggcg cgggtcggca
      421 ttgacacgct ggtgatgggc accgcctttt tccgggcgga ggagcccgaa gcgctggtgc
      481 gccgtgtgca ttctctctga aaaaatggca agaatgagaa gcgtcccctt tatagcagga
      541 aaggacggtc ttctcatgaa accgacgaaa tccaatcagc acggggaagc gctgtttacg
      601 ctctatcccc ggaagaaaac catgaagcgc ggccccgtgc gtcactgagc cgcgctgatt
      661 ggaatgggaa aggaatgaaa ccgttttttg aattttgacg aactgccgct gcttccgcag
      721 ctgttgaccg cggttaaagc ctgcggctat gagaccccca cccccattca gcgggatacg
      781 attcccaccg ttcttgcggg caaagacgtg ctgggctgcg cccagacggg caccggcaag
      841 accgccgcct tcgccctgcc gattctgcag cggctttatc aggagcggcc ttccgggaaa
      901 actcggaata ttcgggcgct gatcctgacc cccacccggg aattggccct gcagattcag
      961 gaaaacttcg tggcctacgg caaagggctg ccgctgcgct cctgcgtggt tttcggcggc
     1021 gtggggcagc agccccaggt ggaacagctc aagcggggcg tggagattct ggtggccacg
     1081 ccgggccgtc tgaacgactt gatcggtcag ggctttatca gcctggccaa aattgaaatt
     1141 tttgtgctgg acgaagccga ccggatgctg gatatgggct tcatccacga tgtcaaaaag
     1201 atcatcgccc ttttgccgga aaaacggcag acgcttttct tctccgccac catgcccgcc
     1261 gaggtggaaa agctggccat gagcatcctt cacgatccgg tcagcgtgaa ggtagacccg
     1321 gtcagcagca cggtggatac catcgatcaa agcctgtatc tggtggacaa ggccaacaag
     1381 aagcatctgc tggccgagct tctgcgccgc ccggaggtgg aaagtgcgct ggtctttacc
     1441 cgcaccaagc acggggccga ccgggtggtg cgggagctga gccgggaggg cattcaggcc
     1501 cgggccattc acggcgacaa gagtcagggc gcccggcagg aggccttggc ccagttcaag
     1561 cagggcaaga tccacgtgct catcgccacc gacatcgcag cccgtggtat cgacattgca
     1621 ggcctgtctc acgtgttcaa ttacgacctg ccccatgagc cggaatccta cgtccaccgc
     1681 atcggccgca cgggccgggc gggacacgaa ggcgtggccg tcagcttttg ctgtatcgac
     1741 gaaatgaagg atctcaaggc cattgaaaag ctcatcggca agcagatccc ccggaaggaa
     1801 agccagtggc ccatgcaggt ctttaccgaa acggtgaagc agccgcccca gccccggccc
     1861 tccaagcggg agcggatggc ggcggaagca agccgttcgt cccagcccgc caagcggctt
     1921 gaaaaggcgg gaaaaccggc cgttaaagcc gcgcttcccg aaaagaggga cttgaagaac
     1981 gcccggatgg agaaaccggc gcggcatgaa aaggcggctg ccgaaaagcc cgttcgggcg
     2041 gcgaaagcgc caagacagca ggagccccag cgccctgcac gggaacccaa gccggttcgg
     2101 gagccgaagc ccctgcaaaa ggctgaacgt cagccgaagg aaacggcgtt tcgcgccacc
     2161 ctgctggaac ggcctaacgc ccggcccgtc cgcccgaagg agggactcat gtttctggaa
     2221 gcgccttttc tggacgacaa gcccgcccgg aaagaggaag aaaagccgtc catgacctat
     2281 cagcagtata tgcagaagca gcgcgccgcc tcccggatgc ggttttccac cggggaagac
     2341 cgccgctgac gaagttgaca aaacggaaac ctgacgtgct gaagaggaaa aagccatgta
     2401 cattgccgat ctgcacattc attccaaata ttcccgggcc acctcctccg actgcgacgc
     2461 gcctcatctg gacctatggg cgcgccgcaa gggcatcggg ctggtgggca ctggagattt
     2521 tacgcatcct gcctggcgtg ctgaactact ggacaaactg cagcctgctg aggacggcct
     2581 gtacacactg cgaaaggagt tcgccctgcc gtccggcgtg gccggcgggg gagaccccgt
     2641 ccgctttgtg gtatccgggg agattagcac catctacaaa aaggacggca agacccggaa
     2701 ggtgcatcat gtgatccttc tgcccggtct ggacgaggcg gaggaactgg cccaccggct
     2761 ggaagccgtt ggcaacctgc acagcgacgg ccggcccatt ctgggcatgg acagccggga
     2821 tcttctgcag atgaccatgg acgcctgtcc ggacgcgatc tttattccgg cccatatctg
     2881 gacgccccat ttttctgtgt tcggggaatt ttcccaattt tccacgctgg aggaatgcta
     2941 cggcgacatg accggctgca ttcacgcg
//
LOCUS       DAWAEA010000164         2967 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5000_length_2967_cov_2.223558, whole genome shotgun sequence.
ACCESSION   DAWAEA010000164 DAWAEA010000000
VERSION     DAWAEA010000164.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5000_length_2967_cov_2.223558"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          511 a         1001 c          903 g          552 t
ORIGIN      
        1 gtacaggcac tgtacgccga agcccagctg gggatcgaac aggaagtagt agatctcctc
       61 cagcacgccc tcggagggca tgttgtccac atcgtgcttg tggggcggga agccggccca
      121 gttgccgggg gtgatgtagg cctcgccgat ggtcagcttt ttggcgttgg tggagccgtc
      181 cacgatgaag ctggtgtccc gctcccaggg cttcacgccc agccggacca cccgcacgtc
      241 cgcctgatga atggcctgag gctcgctgtc cacgtcaatg ggggtggagc agacggcgat
      301 cttgatattc caagggctgt cgatttttac ggagcagttg cggggtgcat agaagcactc
      361 ggccttgggg ttttcgaaca cgttgcggcg tgcgcccacc atgccgtaat ccttgccgtt
      421 ggcggtgaag ttggccttgc cgcccagaat gataaaggcc acttcccggt ctttcgtctc
      481 ataggtgtag ctctggccgt cagaaagctc caaaatgcca aattccagat ccttcaggct
      541 gcacttgccg atttcgcaca gaggggtgta gccgttttta ggggtataag tctttttgta
      601 attcatgctt gctgcttcct ttctttcacg tattgtatcc attcaccgct ggtttcctcc
      661 cagtcggcga cgatgccgta atccgcgcag gcgaagatgg gggcgtttcg atccttgttc
      721 accgccacca ccacgccgga ggagctcatg cccacggtat gctgaaccag tccgctgacg
      781 ccgaaggcca gatacagccg gggccgcacg gtgattcccg tctgcccgat ctgatgagga
      841 tagccgatcc agcccgcgtc cacggcgctg cgagtggctc ccacgccgcc gcccagcagg
      901 gccgccagct tctccagctt ttcgaagccc tttttgctgc ccacgccctt gccgccggtg
      961 acgatcacag gcgcgccctg cagggaaacg ccgctttccg ccggggtgaa ggccaaccgc
     1021 tccagccttt ccgggcttgc cgccagcttc agcggccgca gggcgcagtc tcgcccgtcc
     1081 accggctgag gaatgggaaa aacaccgggg cgcacgctgg ccatctgggg gcgggccgtc
     1141 ctgcaaagga tttccgccgt caggttgccg ccaaaggccg ggcgaacctg tttgagcagc
     1201 cccgcgtccg tcagagacag ggcggtacag tcggcggtca ggcccgtgtg cagtctcgcc
     1261 gctacccatg cggacaggaa ccgcccgcga atggtggccg ggaacagggc cacgtccggc
     1321 tgcagctgcc ggagcgcgtc cgcaatccgc tccgcctgag cgcagtcgtc cgtcagcctt
     1381 cccaacggaa ccgcttcgtc cgcgccaaag gaggcaaaag cggaaagggg cggctccgcc
     1441 gtctcggaca gggccagcac cctgccggac ggggtcagct gacgggcctt gcccagcagc
     1501 tcggcggcag cgcttccgtc gctctcgcac agcacggcaa tggtgatcgc agcggaattt
     1561 tcaatttcag gggaagccga agcagccggg gccgcttcgg cctgcccgga cgcgcccagc
     1621 gccagtcctt ttgcttccag cgctttcatg acctccgcaa aggaggtctt gacgcagggc
     1681 cgaacgccca cgggccgggc gctgatatgc gttacccggg tgggcgacgc tttcaggccg
     1741 cagtcagccg ggtcaatgcc caaatcctcg ggttcgcaga tgcgcacctg agcctgagcc
     1801 gcccgccgca gcccggaaat ggttgggaga cgcagcgcgt gactccattc gcacagggtc
     1861 ataaccgcag gcagcggagc tttccattga aggctccccg cttcggtcag ttggatggcc
     1921 ttaagacagc cgtccgcaat ctccccctct gtggcgttta ccacgcaggg tcgatccaac
     1981 atggccgcca ccatagggcc gacctgcccg gtctcgccgt ctatcgcccg gcggccgcag
     2041 aggatcaggt caaacccgcc cagcgccttt tcggcctgtg ccagacagtg agccgtcacc
     2101 agcgtgtccg cgcccgcaaa tcggggtgag gtcatcaaca cggcttcgtc cgcgccccgg
     2161 gacagtgcct cccgcagcat cccttcggcc cgtctggggc ccatggtcag gacggtcacg
     2221 gttccgccgt gctgctcctt cagctccagc cccagctcca acgcgctttc atccgccgga
     2281 ttgagcacct gcgccacaaa ctcccgttcc agcgtcaaat cgccggtgag cttcacttcc
     2341 gtggtgtcgg gcacctgctt taagcaaatc aggattcgca tggtttctcc ttagagtttg
     2401 tgataaatgg gggccagcgc cgcctgaatc tccttataga gcttaaagtg ctcgttgtag
     2461 aaatccacgc cctccgggtc gggcagcgtc acgccctgct ccctgacgca gcgctcgacc
     2521 gcgtccgcat ggctggcgaa cacacccacg gccacacctg ccaacatggc gctgcccaag
     2581 ctggaatcca cgttctccac cgttttcagg gggatattca gcatatcggc cagaatctgc
     2641 cgccagagac cgcttttcgc gccgccgccg atggccacgg cctgcgtggg ggcaatgccc
     2701 agctccacca gcgtggcgaa gcagtccttc atggaatagg ccacgccctc cagcaccgcc
     2761 cggttgaaat gggccttcgt gtggaagccc gccgcgccga cgaagctggc ccggagcctg
     2821 tcatccagat agggcgtgat ctcgccctgc agataggggt ggaagaacag cccttcgcag
     2881 ccccgggata cctcctccgc cgcccggctc atctcgtcaa agctgccgcc gaaggtgtca
     2941 cggtaccagc ggttgctggc agcgcag
//
LOCUS       DAWAEA010000165         2965 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5005_length_2965_cov_1.743986, whole genome shotgun sequence.
ACCESSION   DAWAEA010000165 DAWAEA010000000
VERSION     DAWAEA010000165.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5005_length_2965_cov_1.743986"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1900..1909
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2347..2367
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          632 a          904 c          879 g          519 t
ORIGIN      
        1 ccgctgcacc atgagctgtc cccacgcggc catccgccct gtgctgctga ccgaagagga
       61 gaaggcgcag gttcccgccg ggtttgtcac cgcaccggcc aggggtctgg gcaaggccgc
      121 tccggcgtat cacttccgta tgcaggtatc gccctacgat tgcctgggct gcggcgtctg
      181 cctgacggcc tgtcccgcca agggcgcact gaccatggct cccttcgagg agatgaaagc
      241 cgagcagctg ctgtttgatc aggtggccat gaacgagacc tatttgaaga aggacgtcat
      301 cagcgataag agcgtgaaga acgtgcagtt cgccaagccc tatttccagt tctccgccgc
      361 ctgtgcaggc tgtgccgaga cgacatacat caagctcctg agccagctgt tcggtgacca
      421 catgtatgtg ggcaacgccg cagggtgctc ctccgccatc agcggcggcg cacccatcct
      481 gccctactgc aaggactgcc acggtcacgg ccccgcatgg gagcactccc tgttcgagga
      541 caacgccgag tttgcctacg gcttcttcca tgcgcaggac gccatccgca aggagctgct
      601 gatccgtctg gacgccatca agtccgaagg tattgccgca gaggctgtgg aggcatacca
      661 gaacgggtgg aataagcacg agacctcccg cgccgtcaca gacgtgctgc tggccgctct
      721 ggaaaaggcc gagcagacgg aggacgtgaa atacatcctt gaaaacagag aatatctggc
      781 caagaagtcc gtctgggctg tgggcggcga tggctgggcg tatgacattg gcttcggagg
      841 tatcgaccat gtcatggcgc agaaccgtga cgtgaacctg atggtactgg ataccgaggt
      901 gtattccaac accggtggtc agtcctccaa ggccacgccc atcagcgcca cggcgaagtt
      961 ctccgccggc ggcaagcagg tagccaagaa ggatctgggc ggcattttga tgcagtacgg
     1021 ctatgtatat gtggcccagg tggccatggg cgccgaccag gcccagacgc tgaaggccct
     1081 gcgggaggcc gaggcctacg acggcccctc catcgtcatc tgctactgcc cctgcctgga
     1141 gcagcacatc aaggccggca tggggaactc ccagctggag atgaagaagg ctgtggagtg
     1201 cggctactgg catctgtatc gctacgatcc ccgccgcatc gcggagggaa agaatccctt
     1261 ccagctggac tctccggagc ccgacagcga aaagctcatg gacttcctca tgggcgaaaa
     1321 ccgcttcgcc gcgctgaaga acaacttccc ggagagagcg gacgcactct atgccaaaga
     1381 aatcgccgat gtaaaggcgc gatacgccaa gtataagagg atggcggagg aaaactgaag
     1441 cctgcttccg tatactgagc gatcacagcg ggggaatcgc tccaaccaag cgattccccc
     1501 tttttttcaa accatttgat aattgccgca aggagacgac tctcatgtat cagactatcc
     1561 gttatgaaaa gcaagatcaa atcgccatcg tgaccattga ccggcccgag gctctgaacg
     1621 ccctgaatgg caccgtcatc acggagctgg aactggtggt ggcggcactg gagaacgacc
     1681 gcgacgtccg ctgcatgatc ctcaccggcg agggccgctc cttcgtggcc ggtgccgata
     1741 tcggcgccca gtatccgctg aatctggatg gcggccgccg ctggggtcag cggggcagcg
     1801 ccctgttccg ccgcatcgaa aagctggagt tccccaccat cgccgctgtg aacggctttg
     1861 ccctgggcgg cggctgtgag ctggctctca gctgtgacan nnnnnnnnna cccagcgcct
     1921 gccccgccgc gtgggcgtgg ccaaggccaa ggagctgatc ttctccggca agatgatcaa
     1981 ggccgacgag gcggaaaaga ttggccttgt caacgccgtc tacgcccccg aggccctcat
     2041 ggacggtgcg ctggagatgg ccaagtcctt caccaagaac gctcccatcg ctgtgaagta
     2101 tgccaaggtc tgcatcgacc ggggtatgca gatggacatt gacgacagca ttgccgtgga
     2161 gaacgagctg ttcgccatgt gctttgccac cgccgaccag aaggagggaa tgggtgcgtt
     2221 cctggagaaa cgttccgcgc agtttacaaa tacctgacag gaggaaacga caatggcact
     2281 gatgaccgcg aaagaataca tcgactccct gcgtaagctg aacacccgtg tgtatatgtt
     2341 cggcgannnn nnnnnnnnnn nnnnnnnggg tggatcaccc tatgatccgt ccctccatca
     2401 actgcgtggc catgacctac gcgctggcgc aggacccccg ctacgaggag ctcatgaccg
     2461 ccacctctag cctgacgggg cggaagatca accgcttcac ccacctgcac cagtccgccg
     2521 acgatctggt gaagaaggtg aagatgcagc gtctgctggg gcaaaagacc gcctcctgct
     2581 tccaacgctg cgtgggcatg gacgccttca acgccgtgta ctccaccacc tatgagatcg
     2641 acgagaagtg cggcacaagc tatcacgaga atttcaagaa gttcctggtc tacgtccagg
     2701 acaacgacct caccgtggac ggcgccatga ctgatcccaa gggcgaccgc tccaaggcgc
     2761 cccacgagca ggccgacccg gatatgtacg tccatgtggt ggaacgccgc gatgacggca
     2821 ttgtggtctg cggcgcgaaa tgccaccaga ccggctccat caacagccac tggcacatct
     2881 tcatgcccac catcgccatg ggcgaggcgg acagggatta cgctgtgtcc ttcgcctgcc
     2941 ccaccgacgc cgaggggctg tacat
//
LOCUS       DAWAEA010000166         2936 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5056_length_2936_cov_2.712947, whole genome shotgun sequence.
ACCESSION   DAWAEA010000166 DAWAEA010000000
VERSION     DAWAEA010000166.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5056_length_2936_cov_2.712947"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          587 a          994 c          918 g          437 t
ORIGIN      
        1 cgctcgcccc ctgcacccct tcgggcctcc ccggtgcatc gcgacaaaaa gcttgcgcct
       61 gcgggcgcgc gggagctttc ctcggggcaa accattggcg catcgctccc ccctgatgga
      121 aaaattcgtt tcccccatcc ccttttcccc aaaggaggtc tttcccccat gaagatcatc
      181 gccctcaccg gcggtatcgc ctgcggcaaa accacgctgg caaatatgct ccgtgagctg
      241 ggcgcgcccg tcatcgacgc agacgccatc agccgaggcc tcaccgcccc cggcggaaag
      301 gcccttcccg ccctccggga ggccttcggc ggcgaggtct ttcaggacgg ccagaccctg
      361 aaccgggccg cgctggccgc aagggtgttc caagacccgg agcagcttcg gaagctcaac
      421 gccgtcaccc atccgctcat catcgaacgg atgcagagcg aaatcgagcg ctgccggaaa
      481 tccggcgcgc ccgtcgttgt attggatgtg ccgcttttgt tcgagaccgg ccttgaccgg
      541 ctggcggacg aaacggtgtg cgtttccgcg cccctgccgg tgcagctgaa gcggctcatg
      601 acccggaacg gcctgacgga ggagcaggcc atgcgccgca tccaaagcca gatgcccttg
      661 tgcgaaaagg aaaagctggc cgaccgaacc gtctccaccg actgtccgct ggaggagctt
      721 cgccccagga tgggccgcct ctaccggcaa tggctggagg aagcccaagc ataaaggaga
      781 gcctatgatc catcccccct atcccctccg ctgccaggac gacgacagcg gcgcgcccgt
      841 tgattattcc tacgtgtcct cggtgaacga aggccgcagg cgggcccagc agaagaatat
      901 ccacgaggag gacgaacagg ccgcgccccg gcccctgatc cgccaccggg ccgaccccta
      961 cgaccgggag cgggtagacg cttacggcac ccgcgcgccc atgcggtatc cggaacgcac
     1021 cggcaccgtg cagatgctgc ccacccggga ggaagaagcc atcccggaga acccctacga
     1081 ccgccccgcc gcgcgccgca ggagccagcc cctgcaggag ccgcaggtca tcgtgacgga
     1141 ggacggctcc cagttcgacc cgcccgccat ggccaagctg cccgaatggt accgggtagc
     1201 ccagcagaat gcctccacca ccgacgatcg gatgcgtcgc gcccccaagg tacaggctgc
     1261 gccccgggtg gaagtggagg aagatgacca gcccgtgcag tatgatccgc tgggccgtcc
     1321 cgtggcccgg cgggcggaac cccggcagga gcatcgcttt caggggtccg ccgaccgcta
     1381 cgccgaggcg ggctatccgc cggagctttt gcaggaacag cgggagctgg acgaaaagca
     1441 ggaagtcatc tcccgccgcc gtcggcatgg cgcgcagcag gcggtcagcg agcggcggca
     1501 ggaggcgtac cggcgcagct taagcggcga gcctggcagc gaccggcgct cctatccccc
     1561 caaccgggag gaatacgcct atcgccgcca gctgacggaa gaagaacgcc agcgccgggc
     1621 ccaggcggca gaagaagcac tggcccggca ggaagccgcc cgaaatcgcg ggcaggccta
     1681 ccggcaggca gctcaggctt ggggcgctcc cggtgaagca agggaaaatc cctacgcttc
     1741 cccttatgaa gtgcgggagg agccccgccg cgcccctgcc cggctggaag agccggaaga
     1801 ggaaacgtcg ccccggctcc gcatcccgtg gctgggcatc gccgtgttcg ccatggccgc
     1861 cattgcggtg gggctgtggc tgatgcaact gaatttccag acccagaccc aacaggtggt
     1921 acaggaacgg gcgcaggcgc ggatcgccat ccgggaaaag cacccgctgg aataccggga
     1981 gctgatcgag cgggaggccc gcaagaacaa cctgaatccg gcctttgtag aggccatcgt
     2041 ctttaacgaa agttccttcc gcaccggcgc ggaaagcagc gtcggcgcac ggggcctgat
     2101 gcaggtcatg gaggacaccg ccgccgacat cgccaaggcg ctgaatgtgg aaaactattc
     2161 cttcgatctg ctctacgacg cggagaccaa cgtgaccttc ggctgctatt atctgggcaa
     2221 gctgagccag cgcttccgag gagacccgct gctggtggcc atggcctatc acgcaggcgg
     2281 caatcaggtg cagaactggc tgaacacctc tctgtacagc gccgacggcc agaccatcct
     2341 tctggacaat ctgcccgacg gccccacgaa gcagtacgcc gttcgggtca aacgagattt
     2401 tgccatttac cgccgcctgt actatgacac tttggaggac gacgcatgaa aaaacggctt
     2461 ctggcgcttt tgacgattct ttgcctgctg tccggctgca cggtttcgct ggcggaaaac
     2521 gtgggcgatt cgctggtggt gggcatggtg tccacccgca ccacggagat ccggcccctg
     2581 acccctcagg aggcggggat ggtgtccctg tataacgtgg tgtacgaaag ccttgtcacc
     2641 gtggacgaca acggaattcc ccagccctat ctggccgaaa aatgggagga gagcaacagc
     2701 ggggagacct ggaccttcac cctgcgggaa aacctgcgtt tctccgacgg ttcggccctg
     2761 accgcggacg atgtggtagc cagcgcccaa tacctgctga cgctggccaa aaacgaggaa
     2821 gccgaggaca acggcttcta ccagaccctg cgctacacca tcagctccat cagcaaggca
     2881 gacgagcgca ccgtcatcgt caaggccgcc cggaaatact acggcgtgct ttacag
//
LOCUS       DAWAEA010000167         2901 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5113_length_2901_cov_3.154603, whole genome shotgun sequence.
ACCESSION   DAWAEA010000167 DAWAEA010000000
VERSION     DAWAEA010000167.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5113_length_2901_cov_3.154603"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1866..1875
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          532 a          911 c          994 g          454 t
ORIGIN      
        1 tggacttctc cgcgctggtg gggcaggcgg ccgccgagcc ggaggacggc ttccgggacg
       61 gggccttcgg ccagtgcgtg gccctttgca ccagcggcac caccggccag agccgggtct
      121 tcgtctatga cggcgaggcc atcgccgagc aggtgctcaa ttccaagctg atctatgagg
      181 aaaaccgacg catcgtggag gaggccaacc ggcgctcgct ggcgtttttg cccttccatc
      241 acgtgctggg cttcatggtc gatctgctgt ggtgcagctt tctgggctat gccaacatct
      301 acctgaggga ccgcaccccc gcgaccatca tcgaaacctc ccgccgcttc cagccggagc
      361 tggtggtggc cgtgccgctt ctggccaaca acctctgcac ctccctgcgc aagcaggtgg
      421 cccgggagag ccgcctgaaa cgggcgctgt tcgcctcggc gaagaatttc agcctgaacc
      481 tgcagcgggt cgcgccggag gcgggcctgt ggctggcgga gaagatcctc ttccgccgca
      541 ttaccaaaag catgctgggc acggaggtca agtgcatcat tctgggcggc agccacacgc
      601 cgcaggagca gctgaagctg ctcaacgctc ttggctatta caccgtctgc ggcttcggca
      661 tgacggagac cgccgtcacc agcgtggaga tgagcaagcg gctgaagaag cgggtcagcg
      721 gctccgtggg caagcccctg cgcagcgtgg aataccgcct gaaggatcag gaaagcagcc
      781 gggttcgccg gggcgaaatg ctggtgcggg gcaaaaccgt tcacaccggc cgcctgcacg
      841 acggccggat gctcccgccg gacacgctgg agggcggctg gtaccccacc ggcgacatcg
      901 tccggctgaa gcggggcgac cgggtgtttg tggagggccg cagcaaggag gtcatcatca
      961 acgaatccgg cgaaaacgta tacccggacg agctggagga gaccttctcc gggctggaaa
     1021 gcgtacatca gtacacggtg ctgggcctga aaaagcccgg cagggatcag cactatgagg
     1081 acattgctct ggtcatgagc gtgggcgacc gctacgggga cggggaggcc atggaggcgc
     1141 tgcggggccg ggtggcccag cggaaccgga agctgcccgc cgtgaagcgg ctgacccggg
     1201 tgctggtcac gccggaggag ctgccgctgg tcaacggcat caaggtaaag cggatcgccc
     1261 tgcgggagat gatcgcccag gagcggctga cctgccggga attgtccctc agcggcgcgc
     1321 cccggatgga ggaggaaaag ccggacggcg ctccggacgc ggccgccggg caggacgacc
     1381 ggattcgccg caccgtccgc aggctgtacg ccgaggcgct ggacgtgaag gagggcagct
     1441 tcggcgacga cgagcatttc atcgacgatc tggggggcga cagcctgcag gtcttgtcgg
     1501 tttccctcaa ggtggaggag cagttcggcg tgatgctttc cgccgaggaa tacggccagt
     1561 gcaccacggt gaacgacctg acccgggtca tccggaacca cctgaacggc gggacggagg
     1621 aaaaagcgga ggagggcggg cctgttgccc ccattacccg ttttgaggac gcgccggaat
     1681 atcaggcctt cgcccggcgt cagcaggagc tgaacgcctc cggcgagggc aatccctact
     1741 tcgtctgcca tgactctccc ctgcgggata ccagcctgat ggccgggcag gaggtgctca
     1801 acttcggcag ctacaactac gtgggcatga gcggccgcaa ggaggtgcag gaggctgcca
     1861 agtccnnnnn nnnnncgcca gcggcagccg cctgctggca ggcgagaaaa gcctgtatca
     1921 ggaattggag cgggaaatcg ccgactggaa gcacgcggag gacgcgctgg tctgcgtggg
     1981 cggccattcc accaacgtga cggtggtggg caacttctgc ggcaagggcg acctgatcgt
     2041 ctacgacgcg ctggcccata actccatcga gcagggctgc cgcctgagcc gggccgtttc
     2101 caagcccttc ccccacaacg acccggaggc gctggaaaac atcctgaagg tacaccgctc
     2161 ccacttcgcc aaggtgctga tcgtcatcga gggcgcgtac agcatggacg gcgacgtcgc
     2221 cgatgtgccc gcctttgtgg ccctgaagaa gaagtacggc tgcttcctca tggtggacga
     2281 ggcccacagc gcctgcgtca tcggccagac cggcggcggc gtggacgagt acttccatct
     2341 ggagcccacg gacgtcgata ttaagatggg cacgctttcg aaggggctgg gcacctgcgg
     2401 cggctatctg gcgggccgga aatgcctgat cgagtacctg cgctacaatc tgcccggctt
     2461 cgttttcagc gtgggcattt ctcccgcgct ggcggcgggc acgctggagg ccatccggca
     2521 gctgcgtcac aacccgcaga tcatggcgga catgaagcgg aacattcggt gcttcgcaga
     2581 ctgcgccaaa aagcaccgct tcgacgtctg tctggcgggc gagaccgcca ttctgcccgt
     2641 gatggtgggc agggacgagg acgcctttgc tctgagcaac gaaatgcgcc gccggggcgt
     2701 gttcgttccc ccggcggtgt tccccgccgt gcccaagaac agggcccggc tccggttctg
     2761 cgtcatcagc gagcacaaac ccgagcagat cgagcgggcg ctggacgtat tggaagaggc
     2821 ggccgccgcc ctgaacatct ccctcccccg cgtgcagcag gggtagtacc cctgcacccc
     2881 acaccgcgcc catagggcgc g
//
LOCUS       DAWAEA010000168         2887 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5132_length_2887_cov_2.322034, whole genome shotgun sequence.
ACCESSION   DAWAEA010000168 DAWAEA010000000
VERSION     DAWAEA010000168.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5132_length_2887_cov_2.322034"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          514 a          855 c          946 g          572 t
ORIGIN      
        1 tttttccgcc gtcaaaatgg gcgggccttt ttcccttcgc tgcctatcgc cgctcggctc
       61 cctcgcagtt ggcgcgggga catactcaac ccctgcaggg gggatgcttt ggcgtggctt
      121 gtgttttttc gcaaggcaaa aggccagcgg gaaatcaaat ccacgctgac ctttactgca
      181 agccgcgagg cctgttgccg agcggcggta aacctgcgat caagttgctt tcctccgagc
      241 gctctgcgcg catagcgcgc agtccggggt caaggggcac tgccccttgc gcgcagtccg
      301 aaaggggctc agcccccctg caggagggag tttggctctt tgccatcgcg gaggacggcg
      361 aggaagtagg agccgtagcg ctccagcttg gcctgaccaa cgccggagac ctccagaaat
      421 tcgtcctcgg tcttggggcg cttctgcacc atgtctcgga gggaagcgtc ggagaagatc
      481 atgtagacgg gcagatggaa gtgggcggcc agcagggcgc ggagggtgcg gaggttctcg
      541 tattgggcgg cctcccagcc ggtgaagggg atcgactcct gacggcgctt gcgctcctgc
      601 cgggcttctt tggcgggcac gccgtagaga cggcccctgc ccgtgtctac ctcgaagggc
      661 agctcttcgc agacggaaca gctgccgcag tcgtccggcg cttcctcccc aaaatagcgc
      721 aggagaaagg cccgcagaca tcgcttgctg gtggcgtaga aggtcatctg cttcagccgt
      781 tccagatccc ggcgctgcag ctcggaccgc tcgtccgcgt ccagctccgg gttgtcgtcg
      841 gaatgctcga tcatccagcg ggcggtgatc acgtcctgcc cgctgtagag cagcagacag
      901 tccgccgggt cgccgtcccg cccggcacgg cccgcctcct gatagtagct ttccagatcc
      961 cggggcatgt tgtagtggat gacgaagcgc acgtccgcct tgtcgatgcc catgccgaag
     1021 gcgttggtgg ccaccatgat gcgggcccgg tcaaacttga aatcgtcctg attgcggcgg
     1081 cgctcctcgt ccgtcagccc ggcgtgataa cgggtggcgg aaaagccgcc ctgccggaga
     1141 cgttcgcata cgtcctccac gttttttcgg gtggagcaat agacgatgcc acaggcgtcc
     1201 gggtcggctt cttccagata acggagcaga tagcccggct tgctggtggg cttgaccgag
     1261 agaaagcgca agttggggcg gttgtagccc gtgaccagcg tgtagggctc ttccagttcc
     1321 aaaaggcgga tcatgtcccg gcgtacctgc tgggtagcgg tggctgtaaa ggccgccacc
     1381 gggggacggt gaggcagagc acggacgaaa tccggtacct tgagatagct ggggcgaaag
     1441 tcctgccccc actgggaaac gcagtgggct tcgtccaccg ctagcagaga aatgggcgca
     1501 tgggtggcaa agtccagaaa tgcgggggtc tgcaggcgct cgggagccac gtagatgatc
     1561 ttgtacgcac cgtttttcgc ccggcggatg gcctcctgct gctggccggg ggtcaggctg
     1621 gaattgatgt aggccgccgc cacgccgctt tgcttcagcg ccatgacctg atccttcatc
     1681 agggcgatca ggggcgatac caccagcgtg atacccggaa acagcaaagc gggcagctga
     1741 tagcagacgg atttgcccgc tcccgtgggc atcaccccga gaatgtcccg tccgctcatc
     1801 agcgcccgaa tcaggtcttc ctggccctcc cgaaaggagt cgtggccaaa ccattttttt
     1861 agggattctg ttaattccat ggatcagctt tcgcgttgca aaaaatgctc agttgtaggt
     1921 gtaggccagc accacgccgc ccagattgtc cagactggcc tgatagccgc tggccgggtt
     1981 gggcaccggc agggtgaagg agtcgccctc cgcgccggag atacggtaga tctcgtaccg
     2041 cagcgcgccg tcctcgtaga cggtgatgtg gggatgggcc atgaggaact ccagatactc
     2101 ctccagacag tagtccatgg tgcgcataac ggcggcatga gcgctgccca catagcggaa
     2161 cagcagcagc ttggcgctga tgccggtctt gtaggtcttg ctttcccagt cggaacgggg
     2221 gaagccgtcc accgggaagc ggaagacgat gccgtccttc cagcagtttt caatgaaata
     2281 ctggccgcgc ttgtcctcgg agaatttgcc cagcgtgttg gcgatggccg catcccggtt
     2341 ataaaggccc atctggaacg caaagccgga ctgatattcg ctggtgccgg ggtagttgac
     2401 gtccttctta gtctgctcga tcaggatgtc gccggaatac tggctgctga gacgctgcat
     2461 ggcgtcctcg aaaagcttgg tctgctcctc gttgctgcgg tagccctgac ggacgatgta
     2521 gctgtcgtag cctgcttcgg tgcagcggtt gatgagggac agaaggccgc cataggcggc
     2581 ggggaagagc tgcaccgtgt tatcggtgac ggggatcttg tagccgctgg tggagccgac
     2641 gctgatcagc ccttcatagc tgtagtcaga ggggacgctg tgccacgggt tgatcagcac
     2701 cagcccgccc tgcagcagct cggagcgact catggtgacg gtggagacgt tttccagccc
     2761 ggtcttgccc tcgtcctcca cgcgccagac cttgccgtcc agcgtctttt cccacgaggg
     2821 gaggttctcg tcgacggtat cgccggagat gggatcgctg aaagcgctgt tggcgcccgc
     2881 ccgaagc
//
LOCUS       DAWAEA010000169         2830 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5238_length_2830_cov_2.073514, whole genome shotgun sequence.
ACCESSION   DAWAEA010000169 DAWAEA010000000
VERSION     DAWAEA010000169.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5238_length_2830_cov_2.073514"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1173..1182
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          526 a          924 c          807 g          563 t
ORIGIN      
        1 atgcagaagg taaccgatac gcccatctac ctgacgcccg gccgcggaaa gctccatttc
       61 agcgaagaag atatgcgcag gtacgccgag gacgtggccg tgcggctgga atacctcgtc
      121 cggaagcgca atgtaaagca cctggcctat tactgctttt ccaacgaatt gtcccagctg
      181 accaacggag cgctgcaaaa ccgcctgacc acctttcagc gctatcacga gctgctctac
      241 gacgcgttcc agcgccacag gctgcccctg ggcctgctgg ccaccgacgc ggccatggac
      301 tggagctcca tcgactgggc catggagcat atgaacccca tcacctgcga ttactgcggc
      361 cactggtacg aacggggcga cgcctgtgac ctttcctttt accagcgttt ttacgagtgc
      421 tgcgcagaat atgccgtcaa ggccgcgcga aaggaaaagc attttcttct cggcgagttt
      481 ggcgtctcca aggaaaacac cgcgcttcaa ggcaccgccg tcaaggacgt atgcacctat
      541 tttgactccg gcgattacaa gggcctttcc ctcatgctgg cggaagcggt ggcggcggca
      601 gtcaacgccg gcgtttttgc cctcatgagc tggacgttct gcgattatcc cgaccccatg
      661 gtcggcccag ctacaccctc tgcgagccat tcttaggcgg gggcgtccgc atccgctaca
      721 acaaatgggg cctcctgaaa tgggaggagg acggctcttt tactccgcgc cctcattact
      781 ttgcctttgg cccgatcgtc cgttacctga agcggaattc caagatcctg caaaccgttt
      841 cggatgatcc tttcctccgc ggctgcgccg tcctgacccg caggggaaga ctctcccttc
      901 tgctggtcaa ccgttcccgg gaggaaaagc cgctggagct tcagcttccg caagcctcgg
      961 gcctgccgct tcgggtattt ctccaccgga ccgacgcccc ggactggaac cccttcgccg
     1021 accttcccgc ctttcaggcc gaggttgccc cctcctctcc tcgctggatc atgccgcccg
     1081 aatccatcgc cgttctcacc gatgattaca cccccgctcc tcgccatgtg gaagcggaga
     1141 tcctcggcac ggacggccag ctcgtctgga agnnnnnnnn nncgtggatt gggacggcaa
     1201 tcagtaattt ctccgggaag agcagctgct cttcccttct tttctatttt tcattctgcc
     1261 cgccggactt ttggcaaaat tttccctctt tcttcggcga tcccaccaaa gcattgactt
     1321 ttcttccttc atctatcata atgaagcgaa atccctccgg ccttttcaaa gccgccggtt
     1381 ttccatttcg atcattcgac aggagagata ctcatgattc gtaccgatcg ccgcgccccc
     1441 atggagaccc gtccctgcgt cattcagccc ggttttgtgg agaccgccga cggaagctgc
     1501 ctcatttcca ccggcaagac ccgcgtcatc tgcaccgcca gcgtggaaga atccgttcct
     1561 ccctttcttc gagacaaagg acagggctgg gtcacggcgg aatacgccat gctgcccgcc
     1621 tccaccgggc ggcgcaaggc ccgggacggc atcaagaagg acggccgcag tgtggagatt
     1681 cagcggctca tcggccgctc gctgcgtcag gcggtggaca tgaccaagct gggcgaacgc
     1741 accatcacct tggactgcga tgtgctcacc gccgacggcg gcacccgcac cgccagcatc
     1801 accggcgcgt atgtggcgct ggtcatggcc gtggacaagc tcatgaagga cggccttttg
     1861 tccgaaaatc ccatcatttc tcaggtagcc gccgtcagcg cgggcatcgt ggaggacgag
     1921 cccctgctgg atctctgcta cgtggaggat tcccgtgccc agacggacat gaattttgtc
     1981 atgaaccacc ggggagaatt tattgagctg cagggcaccg gcgaaggccg cgccttcacc
     2041 aaccgggagc ttcactgcct gctggatgcg ggccgcgccg gggtgcgccg tctgatgaaa
     2101 gcccagcggg aggccttacg cggcatcggc ggccatctgc tgcccctgcc catggtggtg
     2161 gtcgccagcg gaaacgcccg caagctgcag gagctcaccg ccatgttccg gggcgtgtgt
     2221 cagccagtct ccatgaaagc cgtaggcttc gagggcgaca ttgacgaaaa cggctccacc
     2281 tttgaggaaa acgcccgcat caaggcggaa tgcgtcatga aggagaccgg cctgcccgtt
     2341 ctggcggacg acagcggcct ttccgtggac gcgctgggcg gcgcgccggg catctacagc
     2401 gcccgctacg ccggggagca cggcaacgat cgggccaaca acgagctgct gcttcaaaac
     2461 atggacggcg ttgccgaccg cgcctgccgc ttcgtctgcg ccatggcgct ggccctgccc
     2521 gggcaggaga cccgcgtcgt ccggggcgag tgcgagggcc ggctgctgac cgccctggag
     2581 ggcgagggag gcttcggcta cgatcccctg ttcctctacg agagcggcaa aaccttcgcc
     2641 cagatgtccc ccgaggaaaa aaaccgggtc agccaccgct tccacgccgt cgaaaaaatg
     2701 cgggaagttc tccgggagct gttctgatct tctccgtctt tcccatttca tcccgaaagg
     2761 aagcgctgct ttttccatga agcgtgtcat tgtggtttct gactcccacg gcatggagga
     2821 tcgtctgcgg
//
LOCUS       DAWAEA010000170         2824 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5247_length_2824_cov_2.103648, whole genome shotgun sequence.
ACCESSION   DAWAEA010000170 DAWAEA010000000
VERSION     DAWAEA010000170.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5247_length_2824_cov_2.103648"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          547 a          818 c          889 g          570 t
ORIGIN      
        1 ctatctgctt gctcccactg tggcctttgc cgtgctgctg ggaatcagcc tgacgcagcc
       61 ggagacccgc gaccggcgga tcttcgcatg cctgaccgcc ctgtcggcgt tcctttgcaa
      121 cggggccatg acgctggcga actactatcc cctgcggatc gtattctggc cggtcattct
      181 gatgatcgcc gcctgcgcct tactactgcc gacgctggaa gggccaaaga cagcttgggc
      241 gctgaacggc gcctcgctgc tgctttgttt cctgctggcg gtcgatatgc tacaggcgct
      301 ccccaacgat tacaaccgtt accgccttta cgaggcgcga acggcggaga tcgttgccct
      361 tcgggaggaa ggtgagactg acattcacac cttcggcgtg ttgagccgct ccaaatacga
      421 tatgttcggc cccatcgagc tgggcagcga ctcggacagt tggctgaatc aggcgaccgc
      481 tcgctatttc ggcgtggaaa agattgcggc ggatcgtttt gaataaatca tcacgatata
      541 aggagggttt cacatgaagc aactttttga aggcagcatc accaaagtgc atggcatccg
      601 cgtgggacag gcccagaacg acgcggccaa aaccggcgtg acggtcgtgc tttgcagtca
      661 cgacggcgcg gtcatgcgct cggatgtgcg gggcgcggct ccgggctccc gggaaaccga
      721 cctttgcaaa ccggagaata cggtggagcg cgtgaacgcc gtagtacttt ccggcggcag
      781 cgcctacggg ctggacagtg cctccggggt catgcgtttt ctggaggagc acagagccgg
      841 ggtggacatg ggggtctgca aagtgcccat cgtgcctgcg gcggtattgt ttgatctgaa
      901 ggtaggcgac gctcacgttc gcccggatgc ggccatgggc tacgaggcct gtgaaaaggc
      961 cggcaaggaa gtccatcagg gcgcgttcgg tgctggcacg ggcgcgacgg tgggcaagct
     1021 gatccccggt acggttcccg cccccagcgg catcggcacg gccagcatca cgctgggctg
     1081 cggcgtgact gtgggcgcta tcgtcgcggt caatgcctgc ggcgacgttt acgaagccaa
     1141 tactgacaag gtactggcct gcggccatct ggccgacggc aagccggtgc tgtgcgaaaa
     1201 tctgctctac ggcagcgcgc ccatccccga aatgctgaag atcggtaccc cctccggcca
     1261 gaataccacc atcggcgtgg tcgccacgga cgcagtgctg accaaggcgc aggccctgcg
     1321 catggctacc tgcgcccacg atggctacgc ccgcaccatc cgtccggtgc atacccagat
     1381 ggacggcgac accgtctttg cgctgggcac cggccgcatt gacgcagacg tgaacccggt
     1441 gcagctctgc gccgctgcgg cggaagtgac ggcccgcgcg gtgcagaacg cgatttgggc
     1501 ggtacagcac gcatgatcgg gctgggaatc gatctatgtc aggtggagcg cattgaaaaa
     1561 gccatggcgg cccacgccaa tttcctttcc cgctattaca cggaagaaga acaggcttac
     1621 atcgcgcagc gaggcaaggc tggcccacag agcgccgccg ccatgttcgc cgccaaggaa
     1681 gccctgctga aggccatggg cgtaggatta agcggcggca tttccctgcg ggaaatcggc
     1741 gtgtgccatg atgaaaacgg tgctccccac tacaagctga ccggcgctgc ggcggaaaag
     1801 ttgcgcgtca tgggcggcgg aatgatctat ctaagcctga cccacgaagg cggggctgcc
     1861 gctgcggtgg cggtcatcga acgggcctga aacgatttgc agggctgcgg tattcactgc
     1921 cgcagccccg ttgattttct cattcatttt tcggaggaaa cgtatgcttt ggaacgccat
     1981 tgtacaaaaa gagcccattc ataaaggctg gtcctgcgac aaaaaattct gcgcgcaaac
     2041 cgctgatggg acgaaatacc tgctccggct cacgccccat gaaaaaagcg ggacgcggcg
     2101 gcagttgttt gacctgctgg gccgcgtggc ggaaaccggc cttcccatgt gtctccccgt
     2161 agagttcggc tcatgggaag gcggcaccta taccttttat acctggatcg acgcgctggg
     2221 gatcgaggca ggccgtatcc tgcaaaaaat tcacagcatt cccgcgcccg ccggtcagga
     2281 ggactgggcc agtcgcttcg ggcgcaaagc caatgcgaaa atccagaaat accgtggctg
     2341 cgggctgcgt ttttcgggcg atgacgcggt gatccgctat ctgaccgagc accgggaggt
     2401 tttggagggc cgtcctcaaa cctttcagca tggagattat catatcggaa acatgatgct
     2461 ttgcggcagc cagctaatcg ttattgattt tgaccgtttc gattttggcg acccatggga
     2521 ggagttcaac cgcatcgtgt ggtgtgcgca ggttgctccc ccctttgcct ccggcatggt
     2581 ggacggatat tttgacgggg atccgccccc ggcgttctgg cagtgtctgg ccttttacat
     2641 cgggagcaac acgttgtcct cggtttactg ggccatagat tacggcgaag aagaggttca
     2701 ggtcatgctg cgtcaggccg gggacgttat ggactggtat gacggttttc agcgagtgat
     2761 tcccggttgg tattcctctc tttgaatatt caagaagccc cgcattcttt cgaacgcggg
     2821 gctt
//
LOCUS       DAWAEA010000171         2818 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5262_length_2818_cov_1.951140, whole genome shotgun sequence.
ACCESSION   DAWAEA010000171 DAWAEA010000000
VERSION     DAWAEA010000171.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5262_length_2818_cov_1.951140"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    289..298
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2025..2193
                     /estimated_length=169
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          582 a          820 c          784 g          453 t
ORIGIN      
        1 aagcacgtcc ttgaaggtga agaagatcat gatgccaaac agcagcacat agccgcacag
       61 gtgaacgccc gcttcgatct tctgattgac cggcttgcgg cggatggctt cgatcagcat
      121 gaaaatgaga cggctgccat ccagaccggg gatggggatc agattcatca ggcccagatt
      181 gatggagatg atgaccagca gatagaggta aatttcaagg ccgccctgct gggtctgctg
      241 cgccaccaac gacaccacgc ctacaggccc cgccgcgtcg ttcagcccnn nnnnnnnnac
      301 cacgcggagg gcagaatgga gctggcgggc attttttcat agccctgagc gatggtcacg
      361 cccacccggt aacgttccag ttcgctgtcg tactggggag tcacggtgaa tgtctgctcc
      421 tgaccgtccc ggagaacggt gatttccacc ggggcggagg aagcgtccgc gccgatggcg
      481 ttgctcacgt cctgcacggt accgttttcc acttcggtct ggttgacccg gacaaaaata
      541 tccccggcct gcagcccggc ttcggcggcg ggcattccgg cttcgacctc ctgcaccaca
      601 ggggtggtgg cgaccgcgcc gtaaacgccc atcagcgcaa tggccacgac gaaggccaac
      661 acgaagttca tgcccggtcc cgcaaggacg ctgagcatcc gtttccaaac gggggcctta
      721 ttgtagttgc ggggatcgtc cttcgtgctg ccgttcgggt cgtcgtccgt gtcgccgtag
      781 aacatgcagt agccacccat cggaatggga cggatggaaa aagtagtatc gcttttcttg
      841 ctcttgtgct gccaaatgac cgggccgaaa cccagcgaaa attccttgac agcgatgccg
      901 cacagacgtg ccaccgcaaa gtggccgaac tcgtgcacca tgatcaggat gcctaacaac
      961 aggatcgcta acagaacata caggatcgtc atacggattt cctttccggg ggacggaaaa
     1021 aagcgtcccc gtaaagctag tatatgcggc ccggcatgga cagattcaca gggtcagtcc
     1081 gccgtcagca gggacgcagc cacgtcccgg gcacgacggt cggcctccag aatttcgtcc
     1141 agcgaaccgg cggagagaac gcccagacgg ttcagggtct cctccacgat atcataaatt
     1201 cgaccgatgg tcatgggatt cccgcctgcg gattgtaacg gttgttgtaa caaatgagcc
     1261 acggccacct cgttggcggc atttagctcg cagcaggcag cgccgcccgc ctgcaggcac
     1321 tcatgagcca gccgcaggga ggggaagcgt tccgggtcag gccgctcgaa ggtcaggccg
     1381 gacagggaaa acagatccag cggcgcgccg ccggtgggca ggcgttccgg gtagctcatg
     1441 gcgtacagaa tgggcaggcg catatccggc gtgcccaact gggcgatcag cgcaccgtcg
     1501 gcgaactgca ccccactgtg aatgacgctc tggggatgaa cgaccacctg aatgcggtcg
     1561 tcctccacgc cgaaaagcca cttggcttcc atgatctcca gcgccttgtt gaacagggtg
     1621 gcgctgtcga tggtgatttt ctgacccatg acccagttgg gatgcttcag cgcctgtgcc
     1681 ttggtggcgc tgcggatggc ggctttatcc cacgtgcgga aaggcccgcc ggagcaggtc
     1741 aggatgatct tgtcgatggg attgcccgcc gcaccctgca aacactggaa aatggcgctg
     1801 tgctcgctgt ccaccggcag gagggaatgc tcgcccgcct tccgggctgc ttccatcacc
     1861 agatggccgc cggtgaccag tgcttcttta ttggccagca gcacccgttt gttttgaagg
     1921 aaggcttcca gtacgccttt caagcccgcc atgcccacca cggacaccag cacatcgtcg
     1981 cagtccgtct gtgcggccag cgtcagacat tccgggccga agacnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttaccat gctttcgaac aggtcaccca
     2221 cctgaccggc aaagccgccc agcagaccta ccagcacgtc gccccacaaa gcggggtagg
     2281 gcgcagcgcc gcccagcgag aacagccgtc ccaccaccac ggcgcacaga acgctgccca
     2341 gcagtccgcc gacagaacca gcgacggtct tatgagggct gatatggggg cagagcttgg
     2401 ggccgcccac ggcactgccg atgaaatagg cgaaggtatc gccgcccacg gccactgcga
     2461 acaccatcac cagcagcatt ttctgcaggg aggccgggga atccagcagg ccgatgaggc
     2521 acatacaggg cagcactagc gtaatcatgg gcagaacgct gaccagaata tccaccagat
     2581 cggggtcttc ccggcgcatg acctgcagca ccacaaaaaa gcccagcacg gtcagaatcg
     2641 ggataatagc cagcgaagaa tagaacctca tcgcagggac ggccagacac agggccacat
     2701 aactcgtcca gcaaaccggg cggtggccgc ctaccttcag agcgttcagt tcctcgtgga
     2761 tcgccagcat cgtggccaga aaaacggcta ctgcaaaata ccagccacca aggcagag
//
LOCUS       DAWAEA010000172         2804 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5287_length_2804_cov_1.626410, whole genome shotgun sequence.
ACCESSION   DAWAEA010000172 DAWAEA010000000
VERSION     DAWAEA010000172.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5287_length_2804_cov_1.626410"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2415..2574
                     /estimated_length=160
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          537 a          747 c          762 g          598 t
ORIGIN      
        1 cgggccacga agctgcccat gctgtgcccc aacaggaaat agggcagagc ggggtattgg
       61 gcctgcgttt cccggcgcag agcgttggtg tcttcgatca gcgcgtccca gccgtcgttt
      121 tcggcaaaat agcccttcac cacggcgttc tggccatggc ccagatgggt gtggcccacg
      181 acgatgaaac cggcctgatt cagcgccttg gcgggcgcat cgtagcggtc gatatgctcc
      241 gccatgccgt ggacgaactg caccactgct ttgggctgtc cctctgcggg ccacagtcgc
      301 ttttccagtt cttgcccggt gacggaacga aagcttcctc ggatcgtctc tgccatgaaa
      361 tgctcctcct tcaggcccac aatcgagcgt ctttctttca ttatagcatg ttgcccggaa
      421 aggcacaatg cccattcgcc gggggaagga ggggacgaaa aaaagaccgc tccttgcgga
      481 acggtctctt ggtttcagct cttagtactg ctgattgcga ttgcgctcga agcactcgcg
      541 gcaatacacg gggcgatcgc ccttaggctg gaaaggcacc tgggtggtgc agccgcagcc
      601 atcgcagatc acttcgtaca tctggcgctc agcacgattg gggttctcgc ggttacggcg
      661 agcaacgcgg cactcgtggc agcgggtggg atcgttctgg aatcccttgt cagcgaagaa
      721 ctgctgttca gaagcgggga acacgaattc cttgccacaa tcgcggcata ccagcgtctt
      781 gtcttcaaac attgttggtt ccctccaaat ttggtttgtt ctgtttgccg ttgggtgaag
      841 taacctgcgt cttgggtttc cagcttccga acaaccgggg aaggaacctg taccgcatga
      901 ccctacctag ggcaacacac gcattatatc atgacctgag aagaaatgca aagatttttc
      961 taaaaaactg tgtttctctt ttgagaaaga aagtgaaacg ttgcaaattt cctgtttttt
     1021 ccgttataat agcaggacgg aagggccttt gagaacggaa aaaacaggag gttgactctc
     1081 atgtttgatt atctggactg gcgcggcgac ctgagcttcg aaaacgcccc catcaacgcc
     1141 gttgacctgt tgatcttcgc cactttgtcc tactgccccc tggaacggat gccgggcgga
     1201 ttcacgcccg gaacattggc acagctgacg gcggaactgt atccgccgga cgcggctttc
     1261 cgtaacgacg ctgaaaagca gcgctgccag ctgtggaaga cagcggcgga gagcgagcgc
     1321 ttcggcgggg tgcaggtgca ggagttcgcc tatcatttcg acccggccgg ggaaaagcag
     1381 ttttccgcca cactgttcca gctggacgaa aggacggctg tggcggcctt tcgcggcacc
     1441 gattccaccg tggtggggtg gaaggaggac tttaacatgg gctttgagtc gccggtgccc
     1501 gcccagaccg acgcggtgga atttctgacg gccgccgccc ggcagtttga acggctctac
     1561 acctgcggcc actccaaggg cggcaatctg gcgctgtacg ccgccgtgcg ttcgcctgcg
     1621 gacgttcgcg ccaaggtgct gggcgtgtac agcttcgatg gccccgggtt ggacgatcag
     1681 actgccggca gcccggaata tgaagaaatt tcttctcgaa tctgttcctt cgtgccggaa
     1741 tcttccatcg tgggtatgct gctggattat cacgacgaat ataccatcgt ggacagcgac
     1801 ggcgtaggca ttttgcagca taatcccttc ctgtggcaca ttcgcgggcc gcgtttccag
     1861 acccgggcca acctgacccg ctccggccgc tacgccgatc ggacgctgca cgactttctg
     1921 gcccagtgca cgacggaaga gcggcaggtg atggtggaca ccctatttga attggtcgcc
     1981 gccacgggcg cgcagcgggt gaaggagatc ccggcgcagg caggcggcag caatctggac
     2041 gcgttgctgg gcctttcgga aaacggggga aacacgacgg tcagtcagta aaggcgcgcg
     2101 tcgcgtcccc aatagacaaa accctgcccg gactttgact ccgggcaggg ttttgcatgg
     2161 ctgcggcaaa ggcagcttac aattcgtgat agtacaggca gatgttctga taggtaccgt
     2221 ccttcatcag gaaaccgttg ggaacggttc cgatctgcac aaaaccaagc cttttgtaca
     2281 agtgacgggc atggacgtta ttttcgacta ccgcgttgaa ctgcagcaac ttaaatccgg
     2341 cttttttcgc ctgtgtcagg caatccgaaa ccagcttttc cccatatgtt tgccgcgcac
     2401 cgccgaatca acggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccagct
     2581 gcgggaaagc aacgccttcc gtgacgactt cattccagat acggttcatt tccggaatat
     2641 cctgttcgcg atattgtcga atttccatat catccatgcc tcccagcttt ggtgctttgc
     2701 cctgcgggtt attttctctg cagcttttca aactgatagc gcggtttgcc gaggctcttt
     2761 ttcccatatt cgatggcttc tgccgggcaa tagcagatac aggc
//
LOCUS       DAWAEA010000173         2783 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5333_length_2783_cov_1.873534, whole genome shotgun sequence.
ACCESSION   DAWAEA010000173 DAWAEA010000000
VERSION     DAWAEA010000173.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5333_length_2783_cov_1.873534"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    875..884
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1366..1379
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          567 a          817 c          824 g          551 t
ORIGIN      
        1 aatgccgatg atgatgggca ggctgaactc ccggatggaa gcaacgccca ggatgtacag
       61 ggtcacgatg gtcaccagcg tggtcagcgt ggtgttgatg gtacggccaa ggctctcctt
      121 cacgctcagc tccacgatct cggagcgggg ctgaccggcc agcgcgggct tcttgttgtt
      181 ctcgcggatg cggtcgaaga tcacgatggt gttgttgatg gagtaaccaa cgatggtcag
      241 catggcggcg atgaaggaag agttcatctg gatcacgttg cgcaggatga ccatgaagct
      301 gatcatgatg agcacgtcat gcagcaggcc tgccacagcc gcgacaccgc tgaacaggtc
      361 gaaacggaac gcgatgtaga tcagcatcag cacggcggcg atcagcacac tgaaaatggc
      421 gttgcgaacc agcgtcgcac cggccaccgg gcccacatag ctggccgtgg cgttgtcggt
      481 gtccatgttg ggatattttt ccagcagagt cgtttccaga ccattctgca gattctgcac
      541 ttcgtcctcg tcagacaagg taggaacgcg gatctgcacg gcggtgcctt cggagccgac
      601 ggtggaaacc acgaactcct cgatgccctg cgccttcagc gcttcttcca catcggcctt
      661 atcataggtg gaacccatgt cgtactgcag gttcaggccg cccgcaaagt cgatgccata
      721 gttgatgcca tggccggtca ggctcatgac gacggcgacc agcatgatca cgatggaaat
      781 gcagaggcaa attttcgcgc gattctgaat tttcatctta cttcgcctcc ttcacggttt
      841 cgtccgcagc gggctttacg ttagccgcat acagnnnnnn nnnnttggac cagatgcggg
      901 ccacgcgggt cagcaggaag cgggtgatca ggatcgcggt caacatgctg gcgagaacgc
      961 ccaacagcag cgtggtagcg aagccctgca cggagccggt gccgaagaac agcagcacca
     1021 cggccgcgat gatggtggtg atattcgcgt ccaccacggc gctcatggcg tgcttgaagc
     1081 cgatgcgcac ggcgtggatg gtggcccgtc cggcgcggac ttcctccttc acgcgctcaa
     1141 aaatgaccac gttggcgtcc acggccatgc cgatgcccag gatcacgccg gcgatgccgg
     1201 gcagggtcag ctggatgccg ggaaccacgg cgatgaacag gaacagcaga atgatgtaga
     1261 tgcacagcgc ccagtcggcc accagaccgg ccagacgata gcggaccagc atgatcagca
     1321 tgacgagcag gatgccgatg atggcggcct tcacgctggt gcccannnnn nnnnnnnnng
     1381 cgtggcggat atggtatcca gcttatcctg ccgcagatcc agcggcagcg cgccgctctg
     1441 cagctgcagg gcgtagttct gcgcctgctc ctcggtgaag gagccggtca ccagcacctg
     1501 gccgccgtaa atggcgctct gcacagtggg actcatcagc acgttgccgt ccagctgaat
     1561 ggtgatggcc ttgccgatgc tcttggcggt catatcgccg aagatattcg cgccttcctt
     1621 ggtcagcgtg aagctgatgg cgggcttgga gttttcatcc ttggtggcgt aggcggtctg
     1681 cagggcgctg ccgtccatga agacgttgcc gtcggagtcc aggaactcca gcttggcggg
     1741 agagccgatc agatccagca cggcggcgtg atcggtcaca tcggggatct ccacgcggat
     1801 gccgctgacg cccaccttgc tgacggtggc ttccggatag cccttctcgg tcagacgctt
     1861 ggcgatgata tccatggtgt tctgcagcag gtaatcgaag ttgttgcctt cctcccgcag
     1921 ctcgtcgctc atggtggctt cgtattcgac gtacacgccg cccttcaggt caaggccaag
     1981 tgcgatgctc tgaggccagt tgtccacgtc ggtggtgggc agccagggca gcagcttgta
     2041 caagccgcgg ctatccagcc atgtgccatg cacgcccaga taacccacgg cgatggtgag
     2101 taccagtacc acgcacagag ccagaatggc cgaagccttc ctgctcattt tggcgtggcg
     2161 acgattgttg actttcattg tttgtcaacc atccttccgt ccgtattttg ttttgtatga
     2221 ttttattttc cgcagctgcg gaaagaaaat ccgggaagat cagccgcagg cgacgtcctg
     2281 ccggaacaat aaatacgctt catccagata acggccccgg cgcagcacgc agccgttgga
     2341 gtcccggctc aaaaccagac ggggcagcag gcacagcggt gcgcagaaaa acagcgccgc
     2401 cagcatccaa aaccagcggg acgcactgtc ccccgcatcc gcccacaccg gcagcgcgca
     2461 ggcgctcatc tcaaggccgg tcaggggcag attttcctgg agaaccgttt ccgtttcggg
     2521 cgcaacgacc aacgcgctca aaagcagcag aaaaaggccg cacagcagtg ctaccagcag
     2581 cattttgcca aacttcatac ggccttcccc tccttccggc atatggacgt tccgctttcg
     2641 catgggtcta ttatagtgtg tgaatcgttt tccgtcaagg gaagaaaaag ggaaaggcca
     2701 cgccgccgca gcccttcccc caccgttaat agattttcac cgtcagacct tcgtccgctt
     2761 catattcctt gttgatcgtc tcg
//
LOCUS       DAWAEA010000174         2728 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5462_length_2728_cov_1.612046, whole genome shotgun sequence.
ACCESSION   DAWAEA010000174 DAWAEA010000000
VERSION     DAWAEA010000174.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5462_length_2728_cov_1.612046"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    679..688
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1472..1481
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1913..1922
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          491 a          852 c          736 g          619 t
ORIGIN      
        1 ctgcttgctt ttttgctgat tttgtgtatg atgggaacag tcattccaat gaacggagga
       61 acccttccat gcagaaacgg gcgctggcgg tgcatgatct ttcctgtgta gggcgctgct
      121 cgctgacagt ggcgctgccc attctttcgg cggcgggcgt gaacacggcg gtgatcccca
      181 cggcgctgct ttccacacat acgggcgaat ttaccggcta tacccatctg gatctgagcg
      241 accagctttc gcccatcacg gcccatcttg ccacgcttgg gctgcatttt gacgcttttt
      301 acagcggcta tctggcctcc ggggcgcagg tggagcagat tctttccatg ctcgaccagc
      361 tctgtgactc cgagacccat atttttgtgg accccgcttt tgccgaccac ggcagacgct
      421 actctctgat cgacgagtcc atgcccgccc agatgcgccg tctgtgcgcc cgggccaaca
      481 ccatcgtgcc caacctgacc gaagcggcct ttttgctgga tcgtccttat cccggcgagc
      541 actatgaccg gacgctgatc ggcgattact gccggggact gctggatttg agcgctgaga
      601 acgtcatcat taccgatgtg agcttttcgc cggaggaaac cggcatcgcc attttgagcc
      661 gcagcagtca gcaaaacgnn nnnnnnnntg tttcacggaa cgggcgacgt attcgcctcc
      721 ttcgcgctgg cggggctttt gcaggacatg ccgctgaagc ccgccgccga gcgggcgctg
      781 gacctgaccc atgagtccat cgaatggacg cttcgggaag gccagccgct gcgctatggc
      841 gtgcagtttg aacgggtact gaaaaaactg taaatcaaaa cccgccgggg tcaaatcaga
      901 ccgcagcggg ttttgctttt attcggccag cgccagtttt cgcttggatt tcataaagat
      961 gctcacccaa ttcagcgcca tgccgacgcc ggaaaacacg ttggcgatca ccagccgcag
     1021 cgtgtcgttc atgagatttc ccctttattt ccttggtttg atgatacagg aacccacgaa
     1081 cgggagccat atttctttct tacgatcttc tcgctttttc caaggaaacg ggcgaagttt
     1141 ccccattttc cccgaagctt tcccggagaa gctgtcgccc ctctttttcc caccggctga
     1201 cctgcgtctg agacacgccg aaccgcctgg cgatctcctg ctgactcagt ccgccaaaga
     1261 aacgcatcca cagcacctgc ccatagggcc gtcccagact gcgcagcgcg tcccgccaaa
     1321 ggatctggtt ttcgaaggtt tctgcattgt ccggggcggc ggccaacagg cgttcatccc
     1381 gggtaacgga gctcattcgt tcccgggcgc tgagcatggc ggccagcttt tccggcggct
     1441 ggcccatccg ctgcgccagt tcctccacgt tnnnnnnnnn nccgtccgtt ctcctgctgc
     1501 cattcttccc gccgccgttc cgccgccgtc aacaggcgca gttcctcccg ggggatgttc
     1561 atcggggcgt ttcttcggca cagttcccgc agtgcgccca gtgcaaaggg aatggcatag
     1621 gtggaaaatc gaacgccgcg tttttcatca aagcgggagg cggcccacag gagcgccgcc
     1681 tctgcttcgc cgatcagttc ttccatgggc acgccccggc cttgaaaccg tttggcgcac
     1741 gctaccgcca gcccgaaatg ggcgcggttg ccctcagccg atctcgtcca gcattcgctc
     1801 acggtgcaat cccacctcac cggtgatgta tttgcgcatg gtgacggtcg tgcctttgcc
     1861 gacggcgctt tccacctcca ccgcgtccat aaaggtctgc atgacgcaga agnnnnnnnn
     1921 nnaagcccat gccgctgcgc tcgttgccgt cgccggtgga ataaaagggc tgcatggccg
     1981 cgggcacatc ctcgataccg cagccgtcgt catggacacg aacggtcagg agccgtcggt
     2041 cgccgtactc cgcgcacagg ctgaccatgc cctcctgatc ccggtaaccg tggacgatgg
     2101 cgttcgttac cgcttcgctg acggcggtct tgatatctgc catttccggc agggtgggat
     2161 tcaactgcac cgcaaaagcc gcgatcgcca cccgggcaaa ggattcgttc tgcggacagg
     2221 aagaaaaatg aagctccatt cggttttgca ttactgccgc cctccccttt gatgatgttc
     2281 caccgtgatg acccgctgca ggcccgtcag ccgaaacagc ttttcaatgg gcgactgcat
     2341 atttttaacg ctcagcgtgc cgccccgctg ctgcaggatg cgcagccgtc ccagcagcac
     2401 gccgatcccg gaggaatcca taaaagtcag gtcgcccaga tccaggcaca ggtgccgcac
     2461 gcccgggtcc tccaaaattt catccagctg gcggcgaatt tggggggcgg tgcagtgatc
     2521 cagctcccca gccagcacca cggtgacgct gccgtacatt tccttgacat tttgcatgaa
     2581 aatgaacctc catggttttt gtcctgtgta ggtttcgtgt agggttcacc cttatccttt
     2641 gctgaaaaat gggagcttat gcattctttc ccgtctttcg tgcgcccttt tttacagaaa
     2701 tacgccgggg tcttccgttt ttcttttt
//
LOCUS       DAWAEA010000175         2722 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5476_length_2722_cov_2.373828, whole genome shotgun sequence.
ACCESSION   DAWAEA010000175 DAWAEA010000000
VERSION     DAWAEA010000175.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5476_length_2722_cov_2.373828"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          547 a          762 c          809 g          604 t
ORIGIN      
        1 ctcctcggtc atttcgatgg ggccggcggg gatgtcggcg atgtacttga cgatatgcac
       61 accgtagctg ctgatatagg gagcagacac atcgcccacg ttttccacgc tgaacgcagc
      121 ctccacaaag ggagccacat agttggtgga ggcggagctg acttcatagc cgctggtctt
      181 gtagggctcg gaagtcatgc cggggtcgga cgcagtgccg tcttccttca cgccgtattc
      241 ggcgatcagc tcgtcaaagt ccacgccctc gtcgatcttc gcatagatct cgtcgatctt
      301 gtcagcaata gaggccagaa tggcggcctt agcttcgtcc acctgctcct gggtcacggg
      361 ctccgcagtg ggttcggcag tcgcgtcggc ggcagcggtt tcttccgcag cgacggtgtc
      421 ttccgtgtcc tccgcagctt ccacctgctc ctccagacgg gcctgcagat cctgatagtt
      481 cttcatcagc tcgtcggcca cgggcagcag gatgtgcttc accgcgcgga aaccggcagg
      541 acggtaccag gcgtagtcca tgctgctgtc ggtgccgtag tacatggcgt acatggccat
      601 ctgatccacg tagttgttgt aatccacata cgcggcgatg tcgttttcat acagggcgcg
      661 gtcggattcc accagctcct gatacagggc ctccacttcc tcgtcggtca cggtcacgtc
      721 ctgcacgatg gtagcctcca gcttatcgta gagggcgtac agtttgtagt tttccttcag
      781 gctttcgggg ctgtagccca gatcgttgta gtactgcacg gcttccgcgt tggcggcgtc
      841 cttgtcctcc tggctgcttt catcggtcag ttcggagtgt tgctggctga tatagttcga
      901 aatggcgttg tcccagtcgg tctgggcgtc attttccgca tccgccgctt cttcgtccgt
      961 cagggcgatg cccagctcgg cgatcttggc ctgcataatg acctcgttga ttttgttctg
     1021 catggccacg gcgcggaaca gctccacatt ggcgctgtcg gtcaggtcgt agtagttgcc
     1081 gtaggaggag accagactgt catagaccgg ctccacgtcg gcccaggtca gctcggtgcc
     1141 gttaaccgtg gccagcacgt ccgtatcctg aagctcggcc gcggcagtgt cttccgccag
     1201 cgctgcggcg gagaacagca ggcacaggca gagcaggaga gagagaatct tcttcatgaa
     1261 agcacaatcc tttccttcgg gaaaatggtg ccgcgaaccc ttccgcgtgt gatgctaaag
     1321 attttcagcg gcgaatcttt cccatttcgg gtttcattat cgcacagctt tctacatttc
     1381 gcaagggttt tacctttgaa aggaagaatg ttacagtcta ttcacaggtt gtacacctgg
     1441 cagcgatatg attcgacggg gcgcgtcaaa tcccgcctta tggaggaacg aaggatggaa
     1501 gtgctgatct ggaaagcccg aagaatgctg gaatcgcggc aggaaggccg cgtcctgctg
     1561 cgctgcccca tagcgctggg ccgtgagcct gtcggcccca aggagcggga gggcgacgga
     1621 cgcacgccgg aggggacgta ctacatctgc cttataaaag aagcgggcaa atacggcaaa
     1681 agtctgggcc tgagctatcc ctccccggag gacgcggccc ggggcttcgc ggcgggaagg
     1741 atcgacgaag gagcgcttga cgccgtgcgc cgggcatggg caaaccgggt gcgcccgccg
     1801 tggggcacgg cgctgggcgg agaaatctac ctgcacgagg gcggcacggc ttccgactgg
     1861 acgcagggct gcatcgcgct ggagtcagcg gacatggccc ggctgtttga gcgctggcag
     1921 gacatcgaga ccgtcaccat tcttccctga agaaattccc aaagaagttt tagagggttt
     1981 ctcatccaat tctcatcggt tttgcagggc ttcctcaagc accgggcggc gcggcgtggt
     2041 atgatatctt tcgtcaacag gaaaacaacc tgacgctgga tttgtttgaa ggaggatata
     2101 cctgatatga aagccaacaa tgttttttcc gttctttacc gtacccgggt caaggttcaa
     2161 aaagacgaca cccccattct gaacctgtcg ctgatcttct ccattatcgc agtctgctcc
     2221 gcgccgtggc tggccgtgat cggtctggtg gtggcgctgg tgctgggcta ccgcttctcc
     2281 atcgagcgca acgccgctga attttccggc agctttcagg atgtggtgaa cagcgccgcc
     2341 gccaacgtca agggcgcggt caattccttc accggcacgg aaaacgaaag cgaagaaaag
     2401 aaagacgaag aataataata cacagctcat ggaaaacgtc ctgcagcatt gtcgcaggac
     2461 gtttttgggt tatcaaaagc caaatcaggc cgaccggccc cttttttgct cccaaaaaag
     2521 ttactaagat tggtcgttgg cggatggacc ggcggaacgg gcagcggcga catgggcact
     2581 tcggttacga atacggtcgc tcagacgacc aattcttatt gcatcgccga cattaccatt
     2641 gaaaaatgca gccatctgga aaaagggctg tacgtgggcg gcctgaccgg tctggtaaag
     2701 aagccgggtt ccagcacaac tt
//
LOCUS       DAWAEA010000176         2719 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5482_length_2719_cov_1.843093, whole genome shotgun sequence.
ACCESSION   DAWAEA010000176 DAWAEA010000000
VERSION     DAWAEA010000176.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5482_length_2719_cov_1.843093"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    237..246
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          481 a          796 c          907 g          525 t
ORIGIN      
        1 cgggcggccg gagatcagcg gggcgtaaat cagctgaacg cgctgttttt gacggtctat
       61 gccgggatcg ccctgttggc cctgctgtgc gggctgtgga tcgccggaaa cgcggagctc
      121 tttttcggaa gcaagtattc cggggaggag ctggcgctga ccagaacgct gatggtgctg
      181 ctgacctgca atctctcctg cacgttcctt accacggtgt tcacggcttt tgccggnnnn
      241 nnnnnnacgt atgcttctgc ctgggaaagc tgaaaatgcg cttcacgctc cggggggcgg
      301 attttcgggt gatgggcgaa gtggcctcgt tttccgtgtt cattgccctg aacagtctca
      361 ttgatcagat caactggcag gtggacaagg tcatcctcag cgccgtgcgc ggcagcgcgg
      421 ccacggcggt atacggcgtg gccgcccagc tgaacacgat gtatgccgga gcctccacgg
      481 cgatttccgg cgtgtttacc ccccggatcc accgcatcgc ccaggagccg gaccgcacgc
      541 ggcggctggc gcagtacaac gacctgttca tccggaccgg gcggatccag ttcctgctgc
      601 tgagccttgt gtgcagcggc ctggtaattt tcggactgcc ctttatccgc ctgtgggccg
      661 gagcggggta tgacgacgcc tattacatcg ccctgctgct gatcctgccg gctacgatcc
      721 cgctggtgca gaatgtgggc atcgagatcc agcgggcgga gaataagcat cgcttccgct
      781 ccctagtata cacggcgatg gccctgggca atgccgcact gagcatcccc atgggcattc
      841 gctggggcgg catcggctgc gcggtgggaa cggctctgtc cctgctgctg gccaacggac
      901 tgatcatcaa ctggtattac cagaaaaaaa tcggcctgga tatccgccgc tttgccgccg
      961 gacagagcgg aatcgccctg gtgatgctgg ccatgggcgc ggcgggctgg ctgctgacgc
     1021 ggccgctgga tatgagccgc ccgtgggtgc tgctggccgc cattgcggcg tattccctgc
     1081 tttattttgc ggtatgctgg cgcttcgtca tgaacgaaaa cgaaaagcgg ctgctgcacc
     1141 cgctgtataa actgcttcac aggaggtgaa cggcccatga acacctttct ctgcgagaga
     1201 atatcctgca caggctgcgg tgcctgcgca gccgtctgcg cggccggggc aatagcgctg
     1261 cggccggatg aggagggctt tttatacccg gagacggacg ggagccggtg cctgaactgc
     1321 ggcgcctgcg gggctgtctg ccccgtggcg ggcgagaagg aaagccgtcc cgttctccgg
     1381 gcgctggccg cccgctgccg ggatgaggcg ctgcgccgca gcagctccag cggaggcatt
     1441 ttttcgctgc tggcgcagcg ggtgctggcc cggggcggcg cggtgtatgg cgcggccttt
     1501 gcgccggacc tgcgcagtgt gcggcatata cgcgtggatg aaccggcgca gctggcgctg
     1561 ctgcggggct ccaaatacat acagagcgca ccggaggaca gcttttccga cgcggcgcag
     1621 cagctgaagc agggacgatg ggtgctgtat tccggaacgc cctgtcagac ggcggcgctg
     1681 aagcgttttt tgggggacga cccggaacgg ctgctgacgg tggatgtcat ctgccacgga
     1741 gcggcctctc cggccccatg gcagacctat ctggacgagg cggagcgctc tgccggtgcg
     1801 cggccctgtg ccgcgaacct gcgggacaag agccgcgggt ggaaaagatt tggcgctgca
     1861 ctggacttca gcgaccggac acacgtgtac ggaacccagt tggaggaccc gtttctgcgg
     1921 gtctatcttc agaatgccgc gctgcgggag agctgttacg gctgcccctt caagggggag
     1981 cattttgccg gcgacatcac gctgggcgac ctttggggca tccgccacct gtcgctgccg
     2041 ccggagctga acgatgacgg aggcacgtcc ctggtactgc tccgttccca aaagggccgg
     2101 actctgtggg aaagcgcggc ccgggaggcc ttatccgcag agatcgacgc ggcgcaggcg
     2161 ctggcctgca acagcgcggc actgcggccg gtgaagcggc cggccgcgcg ggacaggttt
     2221 ttctccctgc tgcggcagga gggcctgcag gcggcggcaa agcaattgtg cccgctgtcc
     2281 ttcaaggagc ggatcaagcg cctgcttggc cgctgataac cgtatttttt ccgggagctg
     2341 tgacccggat aaaatagaat ttgaatgaaa ggtgagagaa catgttgaaa accaacaagc
     2401 cgtggactcg tgccctgtcg actgcgttgg cactgtgtat gctgctgtcg ctgctgccgg
     2461 tgtcggtgct ggccgtccag cgcgaagcca caaccgagac ccagagtgat gcatactact
     2521 ccgtgatctc ccagaagaac tataaggttt ctcctggcat cacggagaag gagatcattc
     2581 tcaacaacag cacaggcacg gatcagaacg tatgccatgt gctggaagca gatctgagca
     2641 atccctatgt aaccgtgatg cccagctata aggatatgaa gctggacagc tgggtcaccc
     2701 ctatggcaga gtccgattg
//
LOCUS       DAWAEA010000177         2717 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5490_length_2717_cov_1.688204, whole genome shotgun sequence.
ACCESSION   DAWAEA010000177 DAWAEA010000000
VERSION     DAWAEA010000177.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5490_length_2717_cov_1.688204"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    748..757
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          535 a          663 c          911 g          598 t
ORIGIN      
        1 acatgaagct tggccgtggt tttaccgccg tggcacaacg ggtgccacgg ctcgccgatt
       61 tgatagggcg atgcactgca ccccggactg cgtgccatgg gcgcgcaggg gcgtagggga
      121 catgaagctt ggccgtgttt taccgccgtg acataacggg tgccacggcg ccggtttggt
      181 agggagatgc cctgcacgcc ggacagcgca ccatgggtgc acaggggagt agcgggacat
      241 gaagcttggc cgtggtttta ccgccgtggc acaacgggtg ccacggaaga acgtgttggc
      301 ggagggagta ttgccgccag gccaacgggc ctcacgattc tcgtgagagg cgattttccc
      361 ggcaggatgg gccgaaatac gcccttaggc taacggcaga gtggtatttt ccgccgccgg
      421 gctttcttcc cggcggcgga aaaaacgaaa ggagggggag agatgcaggc acaaaggaaa
      481 catcggcttc attggaagca atggttatgg ggtattccgc ttggactgct ggcgatctgg
      541 caatggacgc ttttttccaa tatgatccgg caattatttt tgggatttct ggtcatgctg
      601 gcagcgctgc ctctgatgaa ttttctggaa aaaagagtct caacaggctg ggcggctacg
      661 ctggccatgg cggggctcag cgtgggagca gggttatttc tgttgctgct gatcccgcca
      721 ctggtgggtc aagcacggca gatcggcnnn nnnnnnngga ctataccggc aggtggggga
      781 gatactggaa caggcggaag cgtggcttca gagaaacggc gttgcggtag atcagcgaat
      841 gcgggaaagt ctgttggaaa agggcgaagg gcttctttcc gacacggcgg gttctctgac
      901 aaaacggtta ggcggcgtag caggcgggat cggccgttgg atgctggctc cggtattcgc
      961 cttttatctc ctgcgggatc ggcggcgcgt tgccgactgg ctgctgtccc tccttccgcc
     1021 gggacgccgg gagatgacag tgcgcatcgt ccgggaaatg ggccgggaaa gcgtgggcta
     1081 tctgcgggga caactgctgg tatcgctggc ggtaggcggc tttaccgggc tggggcttct
     1141 tttatgcggc gtgccgtcgt ggctgctgct gggactgttg atgggcattc tggagtggat
     1201 cccctatgta gggccttttc tgggcggcgt gctggtggcg ctgttcgcat ggcccgaggg
     1261 ctggagtcgg atgctgtggt cgctgggtgt ggtggtgctg gtgcagcaac tggagggcgg
     1321 aatgctgtcg cctcagttga tgagtgagac gacccggctg catcccatcg tggtgctgtt
     1381 gtgcgtcatg gcgggcggcg cggccggggg cgtggcggga gtgctgctgg ccatcccagc
     1441 ggtgctgtgt gtcagggccg ccctgcgggt catccagctc aaacggctgg aagaacagtt
     1501 tgaggaaacg taacgcgccg gattcccctt gcggaggaat ccggcgtatc tgtatatcgt
     1561 cgcggcaaag ccgcgctgcg gtttgtcgcg ctcggaacgg agggtggaga acgcgcgggg
     1621 aacagaagga atttggctcc ttatgaatat cgtcccggtt tgccgcccgg acggccttgc
     1681 accgccgtcc gagccatctt acgtaagagc ttccctaagt gccgcatccg aagcgcattg
     1741 gtttgtcgct tccttgtcga caccatcaga ataccagaca aaaatgagga aatcgtgaag
     1801 aaacgggaaa ggttttttga agcctgtgag gaaaacggca gaaaagtcgg tttacggctt
     1861 cttttccggc ccaagggcgg aggtgtctac cttctgctga tggatagcat acagtcgggc
     1921 atagtgttcc gcttcattgg aatttgtgac cgcggaaggg atcagcccgg tgcattccgt
     1981 ggcggaactc aggttttcta cttcggtgat agggggattg ggaaggactg tttttctctc
     2041 ttttttcatg gtcaaacctc ctgacggggt tcagtttatc ccaaaaagaa aacaaagaaa
     2101 catggaaaag tataggcaat gttttctttt gaccattttg ggcaggggag gtcttgaaga
     2161 agggcgcagc cttgcttttc agggcgcggc ggtctataat ggattggcta ccttcagcag
     2221 cggaggagaa atgcagatga aacttttctg ggatgacaaa cctttttatc gcggaaacac
     2281 ccactgccac actactcgca gcgacggacg gcgcagccct gaagaggtga tcgcccttta
     2341 ccgggaggcg ggatacgatt ttctgaccct gaccgatcac cgcaagctgt cggagcagga
     2401 gcatttcgaa gaaaatctgc tgatgatccc cggaatggaa atggattttc tgtatcccac
     2461 tcaggcgctg catatcgttg gcatcggcat gaacgagtcc ttcgcccagc agcgggaaaa
     2521 gtggtgcgag tcgcctcaaa gctgtatcga caccatgcgg gcctgcggcg gattggcgat
     2581 tctggcgcat ccgcactggt cgctgaatac gctggatact atgcgcagcc tgaagaacgt
     2641 gacggcggcg gagatttaca ataccgtttc cggcacaccg tggaacggcg ttcgggcgga
     2701 cagttccaat attctgg
//
LOCUS       DAWAEA010000178         2713 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5495_length_2713_cov_2.285929, whole genome shotgun sequence.
ACCESSION   DAWAEA010000178 DAWAEA010000000
VERSION     DAWAEA010000178.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5495_length_2713_cov_2.285929"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2135..2144
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          479 a          817 c          952 g          455 t
ORIGIN      
        1 gtgatcgaca tggactggca cctgaccgac gtgccctatg gcagcggctg gacgggctac
       61 acctggaacc gggagctgtt ccccgacccg ccccgcttcc tgcgggcgct gcacgaccgg
      121 ggaatgcgcg taacgctgaa cctccatccc gccgggggcg tgcagcccca cgaggaagcc
      181 tacgaaagcg tctgccgggc gctgggccgg gatccggaaa agaagcaggg cgtggatttt
      241 gatgcggcgg acgagggatt cctgcgggcc tatctggact gcctgcatca tccgctggaa
      301 gaagaaggcg tggatttctg gtggatcgac tggcagcagg ggaataccag cgccattccc
      361 gggctggatc ctctctgggt gctcaacgag ctgcattatc gggataacgg tcgggacggc
      421 aagcggggcc tgatcctgtc ccggtatgcc ggccccggca gccagcgctg cccggtgggc
      481 ttttccggcg ataccatcat gagctggaaa tcgctggcct tccagccccg gtttaccgcc
      541 atggcggcca acgcgggcta cccgtggtgg agccacgata ttggcggaca catgaacggc
      601 gttcgggacg acgagctgtc cgtccgttgg ctgcagctgg gggttttttc gcccatcctg
      661 cggcttcatt ccagctgcag cccctttacc ggaaaggagc cgtggagcta tgggctggaa
      721 gcggagctgg ccatggagga cgcgctgcgc ctgcgccacc ggctgctgcc ctatctgtac
      781 acggccatgg agcgcacctt ccggctgggg gaggcgctgg tgcggcccct ttattaccgc
      841 tggcccgaaa gcccggaggc ctactccgcg cccgatcagt acctcttcgg cccgtcgctg
      901 atggtctgcg ctgtcacccg gcccatggac gcccggctga aaagggcctc tgtcaccgcg
      961 tggctgccgg aggggcgctg gtttgacttt gccaccgggc aaagctatac cggcggacgg
     1021 ctgatgaagc tgtggcgcgc gctggatgaa tacccggtgc tggccccggc cggggcgatc
     1081 gttcccctga gcgaagagcc ccgggcggac atgatgcctg cggaaatgac cctgcggatt
     1141 tttgccgggg cggacggggc cttcgacctg tacgaggacg acggcgagag cgtggagggc
     1201 gagacggtgc gcacggccct tcggttctcg tggaaaaccg gcgagctgac cctgcgggcg
     1261 gagggcgcgc tgcatctgct gcccccctcc agaatctggc atgtggaggc cgtcggcttt
     1321 gccaaaacgg cggtcagcct tgagggaaaa gcgctggaaa cggcctgcga tccggctcga
     1381 aacgccctct gctggacggt ggagctgcct gtggacgacg cgcttcacac ccttcggctg
     1441 gaacgctgcg ccgtggcgga ggacgactgg aagcgccggg cggaaaaacg gctgcaggcc
     1501 atgcagacgg ccaacgatga gaaggaagcc gtctggcggc tgttgaatca gaaaaaacgc
     1561 ggcgcttccc tgctgggcac gctgcggctg acggcgaaaa cgccgggcct ggccgaatgt
     1621 ctggaagaat gcgtttttgc gcaggatgaa acgagttttg acgcatgcgg ggaggaaaaa
     1681 ccatgatacg ccatctgcca gaagggctgg tgccctttga agaatgcggc tacgaccgca
     1741 agcccctgca tccgcaggaa ggacaggaaa ttcggctggg ctgtctgtgc gacggcgcgg
     1801 ccccggtatg ggagagcgcg gacggacgaa gcattccggg ccgcccggga acccgggagc
     1861 attgctatac atggtcgctt ccggcggaga agggaaccct gcggtatcgc tttgtcagcg
     1921 gagaggaaag caccccgtgg ttcgaaacgg acattcttcg ccccgtttcc gtgacccggc
     1981 cccttcggac agggcgcggc tgggccgagc tgattcctgg cgtatatctg tgcgccgaag
     2041 gggagaaaga cggcgcggcg ctgacgctgc ggaaggcccc caaggaggcg gaaagccccc
     2101 tgcgctttgg cagcgaagcg ctctggtcgc tgggnnnnnn nnnnggacgc tttcctgcac
     2161 ggaaatcacc ctccttctgc ggccggacgg aaagcccgcc cggatggaaa cgacgctgcg
     2221 gggcggtcag cgccatgtgt acggcaccgg cgagcgattt gacggcccag accagcaggg
     2281 ccggggaagc tgcggacagg tggtggagca tttcacccgg cagggcccgt ggagctatct
     2341 gccgatgccc atgttcctga cggacggcgg gttcggctgg ttccgggacg cgggagaaaa
     2401 cgtccgcctt gcctttggaa cggaggttca tattgagtct gccgcagggg aaaagatcac
     2461 cgaccatctg ctgctgggaa cgcccgccga acagcttcgg gcctatctga atctgaccgg
     2521 cgaatgcatg ctgccgccgg agtgggcctt cggactgtgg atcagcgcca acggctggca
     2581 ctgcgacgcg gacgtggatg agcagctctc catgttgaaa aagtacgatt gcccggcctc
     2641 cgtcatggtg ctggaagcgt ggagcgatga aagccccttc taccgctgga acgcggtctg
     2701 gcctgacccg gcg
//
LOCUS       DAWAEA010000179         2696 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5528_length_2696_cov_2.224158, whole genome shotgun sequence.
ACCESSION   DAWAEA010000179 DAWAEA010000000
VERSION     DAWAEA010000179.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5528_length_2696_cov_2.224158"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          536 a          809 c          798 g          553 t
ORIGIN      
        1 cctcctttgt cgtgactgac ccctgctgat gcgggcagga accgcgttat tataccagaa
       61 aacgccgctg gttgcaatcg tgacgcgggt ttcggcggta ttctgcaagg tttttatggt
      121 cttacgaaat gtttcacgtg aaacaatttg actttcgttt gcgactgtat ttgtcagtgc
      181 tgaaatgttt cacgtgaaac atttcagtct atgctgggac cgattcatgc gttccgatga
      241 tcgaaaattt tcccttgcac ttgatcaaat aaaaacggaa tgtttcacgt gaaacattcc
      301 gtaagggctc agaaaaagga gaaaattaca gcgccgcatc ctgccgcaga agctctgcct
      361 tgtcggtgtg ctcccaaggg aggtcaatgt ccgtgcgacc gaagtgacca taggcagccg
      421 tctgccggta gatggggcgg cggagattca gcattcgaat gatgccgtcg gggcttagat
      481 caaagtgctt ctggaccaga cggcccagtt cgtcctccgg cagcttgccc gtgccgaagg
      541 tgcttacctg cacactgacg ggctgtgcca cgccgatagc ataagccaac gccacttcgc
      601 attcgtccgc cagaccggcc gccaccagat ttttagccac ataccgggcg gagtagcagg
      661 cggagcggtc taccttggaa gggtctttgc cgctgaacgc gccgccgccg tggcgggcat
      721 agccgccgta ggtgtccacg atgatcttgc gcccggtcag acccgtgtca cccgcggggc
      781 cgcccagcac aaagcggccg gtggggttga tgtatacctt ggtgtcctgc ttccagaggt
      841 gctggggaat gaccttgtga atgacctcgg tgagaatgcc gtcttccagc tcccggcggc
      901 tgacctgctc gtcgtgctgg gcggaaatga ccactgtgtc cacggccacg ggcttgccgt
      961 cctcatattc taccgtcacc tgacttttgc cgtcgggtcg gagccaaggc agcattccat
     1021 tcttccgtac ctcggtcagg cggcgggtca gccggtgggc cagcgagata ggcaggggca
     1081 tcaattctgg ggtctggtta caggcatagc cgaacatcat gccctgatcg ccggcaccgt
     1141 gggaagcttc gaccgcctgt ccgcgggttt ccagcgcgtt gtccacgccc atggcgatat
     1201 cgggactttg ggcatgaacg gcggtcagta cggcgcagct gttgccgtcg aagcaaagat
     1261 cgctgctggt gtagccaatg tcccggatca cctgccgggc gatctcgtcc atggcaacat
     1321 aggtagaagt ggtgatttcg cccatcacct gcaccaggcc ggtggtggcg caggtctcac
     1381 aggccacgcg ggccatgggg tctttggcga ggatagcgtc cagcaccgcg tcgctgatct
     1441 ggtcgcaaag cgggcgcgag aaggattgta tttacgacct tatctgccag cgtttgcacg
     1501 ctgcgggaat tgccacctcg cttgcgcagg ttggcgggtt tcatcgggcc cgtccctcaa
     1561 ccactcttca taaggtgttc agttgttacg ggcagtataa cagaggaggc cgcagggtgt
     1621 caataggcat tggcggggga atgtctagtt catgacggaa ctcaggatgc tctcctaaat
     1681 aaaacggctt ttggtcccaa ctacgctcca tatccaaacc agccccatat atgccagaat
     1741 tccagcgaac cggcgctgac ggcggatgcg atcacacagg cagcgaggaa cagtacaaag
     1801 aggatcacca gcaccacgga agccgaccgc agacggagac ggcattcctt tttcagctgg
     1861 ggatgcaggg gcagagcagg cagccccgcc cggttccggg ccgctgccag cgcgttgctc
     1921 cgctgacggc cataggcgcg gcaaagctga cggaggaact cggcatagta caccgtgcct
     1981 acggcactca gaccgcacag agccaacagc atcagcccgg ccagcaccgc gcagaggcgg
     2041 ggcatggagg gcagcagccc ggcgatgttc cagccgaaga tcaggcatat gcccgccacg
     2101 gcgcatacca ccgtaaaggc tgccataacc accagccatg ccgccagcac taccatgccc
     2161 agcgcagcca ccacgtccat ggcccccacg ccgaaacgaa tcagatgggc cgcagggccg
     2221 gagggcgctt ttccgccttc gtccgccgcc tcaaactgcg ccgccagttc cgcgggtgcg
     2281 cccagcttcg cggcgatctc ctcctcgctg aagccgtcgg gcctgtttca gggcaaagtg
     2341 ctgctcgtat tcgcttacca cgtcctccca atcggggatg tgacgcttct gcagttcatt
     2401 ctgaaaagaa gccagaaatt cattcttcgt catgaggatc ctcctccaat agatcccgta
     2461 ccgtttttgg gcggcgtagc tttcccgtcc cagctgggtc agccggtaat acttgcgggg
     2521 tgggccgccg ctttcctcct gcaggtaggt gtccagcatc ccgtcgtttt tcagcttgcg
     2581 caagatgggg tagacagtgc cgtcggccag ctccatacgc tgagccagct ggctggaaat
     2641 ttcgtacccg tagcagtcgc ccttatgcag aagggccagc acgcacagtt ccagca
//
LOCUS       DAWAEA010000180         2696 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5530_length_2696_cov_1.705793, whole genome shotgun sequence.
ACCESSION   DAWAEA010000180 DAWAEA010000000
VERSION     DAWAEA010000180.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5530_length_2696_cov_1.705793"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    372..414
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          508 a          835 c          742 g          568 t
ORIGIN      
        1 gatcctgact caggccgatc agttcctgcg ctttggcctt cacctcgtcc gtccccgtcc
       61 agcattcgcc ggtgtccagc tccgtcagct cagcggcggc cttctgttcc accagatcgt
      121 ccccccggcg gctctggcgc aggtagggcg gattccgttt caggcgatag gtgtgctgac
      181 ggtgctgaaa taccagctcc acataggtat ccgtgtcccg ggcggcatag tccgaccgaa
      241 aggatttgga cgcgcggcgt tccctgcccc cggaaccctc gccgtaaaaa gcgaagctga
      301 tagcgtcgaa aatggtggtc ttgcccgcgc ccgtgtcgcc ggcgatgaga aaaatgccat
      361 tttcgcaaag cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntccgca
      421 gactgcgcag aatctccata tgccgggggc cgggcgggca gtcgttattc tgcagccggt
      481 aaaaatccgt gaacaattcc tccgggcttt tgttctccat ggcttcccgg gccagcacgt
      541 caatatccgt cttggttttg gaattgacca cggaaaactt catcatgttg ggaaatacgg
      601 tgctcaccgt cacccgtgca tccgggggcg gtagctcgtc acgcagcgtg acccacacat
      661 agtcctccga atagggcatg gccatcactt cgtccagcat tccctccacc agccgcatat
      721 cgtgaggggc gttcagcggc acgatatgga cgctgacctc ccctttttcc cccatttcca
      781 ccaccgcgac cgattttttc tggttcacct cggaaaaaga atacttcatc agggaaccag
      841 cgtaacgcag ggtgtcccgg gtgcatttct gaggcttgtg gatgtgaccc agcgccacat
      901 aatcaaaatc ggcaaacacc gccgcgtcga tgttgtccag cccgcccacg gtcttttcct
      961 cggagtcgga gatctccgcc ccggtgatga actggtggca caccagcacg ttccgcttgg
     1021 ccgtatccac cggcgaatgg cgtagcaccg cctgcaccgc gtcctgatag gtctcgatct
     1081 tctcatcggg atagaaccgg cgcacctgta tgggcttcaa aaacggcagc agactgacca
     1141 ccagttcccc gtgctcgtcc tgtagcgtca cctgctgcaa atgaccgtca aaggcctcgc
     1201 tgacgtacac gcccgagctt ttcaaaagcg acgaaaaata ggaaattcgc tgggcggagt
     1261 cgtggttgcc gctctggggc gaggctttct ggtatacgtc cccggcgatc agcaccgcgt
     1321 cggcccgctc acttcgggcg atctcctcca cctgacacag cagccgaacc tgatcctcca
     1381 aaaatgaaaa gtcgttcagg cgtttgccca gatgcaggtc cgccagatgc agaaatttca
     1441 tcgtctgttt ctcctttgta tcccattttt taatgcttca cggactgcct tttccctctg
     1501 tcagcctctt cattgtatcc catcggccct tccctggcaa gacgcaaaat aggcttctgg
     1561 ctgttcccta aaccggtttt tcgtgttaca atgcttcggg cgtatgccca gggaggtttt
     1621 tttcatgatt catgtcagcc agcccatgac caccgtgccg gagcattttt ccacgccctt
     1681 gcaggagctg gtctaccgta ccctgacccg gctctccatt tcctttcagc gggtggaaaa
     1741 cgaccccgcc atcaccatgg aggattgtca ggccatcgac gaccgcctgc aaatgcagac
     1801 cgtcaagacc ctgtttttgt gcaaccgtca gcagaccgcc ttttacctct tcgtcacccc
     1861 gggggacaag ccctttcgca cgaaggattt cagcgccgcg ctgggcgtgt cccgggtatc
     1921 cttcgccacg ccggaacagc tggagcggct catgggcaca gtggtcggct ccgccacggt
     1981 gttcagtgcg cttctggctt cggcccggga ggtgcgcgtg gtcttcgacc gggcgctggc
     2041 ggacagcgaa tggtacggct gcaccgacgg caccaccacc ggctacatga agctgcgcac
     2101 acgggatgtg atcgaccggc tgctccccta caccgcccat acgctggaat gggtggaggt
     2161 gtaaacgcac ggcgcttttc gccgacttca tcccattcga atgccatttt tacctgaaag
     2221 gaacgaaggc ccatgacccc tgaaatcgaa atttcccaag cgacgctgaa cagtctgacg
     2281 aactgtctgc tggtggacat gcgggacgcg gaaagcgtgg actacggggt tatccccgga
     2341 gcggttccgc tgccgccgga acggctggaa gccgaggtgc cctccctctg cgccggaagg
     2401 cccgtggtac tgtactgtgc cagggggcag ttcagcgtgg acgcggcgga agaactgcgc
     2461 cgtctgggcg tggaggccta cagtctggag ggcggctacg cggactggct gctgtatcga
     2521 atgcagcggg agcaggagac cagtctgcag cagcagatcg aggagggtat ccgcaaaacg
     2581 tggcacaagc ggcttttctc caaatttgcc aaggccatcc gcacctacga tctggtcaag
     2641 cccggcgacc gtatcgccgt gtgtatttcc ggcggcaagg actccatgct catggc
//
LOCUS       DAWAEA010000181         2691 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5545_length_2691_cov_1.982929, whole genome shotgun sequence.
ACCESSION   DAWAEA010000181 DAWAEA010000000
VERSION     DAWAEA010000181.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5545_length_2691_cov_1.982929"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1104..1113
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          595 a          728 c          855 g          503 t
ORIGIN      
        1 aaggtacact ggcggcagct tatcattctg gcagagatcg tgctgctggc ggcggtgggc
       61 ttcctgccgg aaacgctgaa caccaccgcc aacgccgtgg tgtccttcgt gtgcgccatg
      121 caggtgcaga ccttccgcaa ggtgcggggc catgcctacg ccagcaccat gtgcatcggc
      181 aatatgcgca gcggaacgga ggcgctgtgc gcctacttcc atacgaggga taaggaaata
      241 ctccatcagg cgctgacgta tttcggcgtg gtgctgctgt tcgcgctggg cgcgggtgtc
      301 ggcagcatag ccacgctcca ttggggcgcg gggacgatct ggctgtcctg cgggctgctg
      361 accgtcagtt ttctcattat gttcatccac gacgaggaac agaagctgaa cgaataaaaa
      421 cagcaccggc ggcgttaccc acacgctgcc ggtgctgctt tgaggtagat cctcatggaa
      481 aagaaaaagt atggttggct gaaccatgtc cttatcatag caggtcgaat agtgtttgtg
      541 aagcgcatct ttgttcttga aaacatgagg acttgtatag tataatgctc ctcgaaacga
      601 ggtgcgccat gagtatttcc aaatatcagg tctttctgaa aactgtctcc tgcggcagct
      661 tctccaaggc ggcggaggcc atgaacttca cccagtccgg catcagtcac gccatcaact
      721 cgctggaaaa cgagctgggc gtgaagctgt tgagccgcaa ccgcggcggc gtggtgctga
      781 cggcggacgg acgggcagtg ctgcccaaga tcgaaaagct gtgcgccgcc caccactccc
      841 tgatgcagac ggtggagggg ctgaaggaca tggacagcgg cttggtgaag atcgccacgt
      901 tctccagcgt gtccgcccag tggctgcccc gcatcctcaa gagcttcggg aaggtgtacc
      961 ccaacatcga gtttgaagtc gtcaccggcg atttctatga ccagatcgag ggatggatcg
     1021 tcaacggaac agtggactgc ggctttctgc gtctgccgtc ggtgaagcgc ttgcagacct
     1081 acgccctgca tcgggacgaa ttgnnnnnnn nnncccctgt gaccatccct acgccgaccg
     1141 cgacccgttc cccgtgtcgg cgctggcaga ggaacccttc atccagctgg aggagggcga
     1201 cgactacgag atcatggcgg cgtttgacga gatgggcatc cgccccaacg tgaagtacat
     1261 cgcacgggag gacaggacta tcttggctat ggtgtcggag ggcctgggca tcagcctgct
     1321 gccggagctg atggtgcgcc acagccccac gcccatcaag gtatgtcatg cgccgctgca
     1381 cttctaccgc accatcggca tcggcgtcaa ggacaagaag gctctttcca actccacccg
     1441 tctgttcgtg gactatgtgc ggacgtgggt ggcggaaaac gggaatacat aaacgttcaa
     1501 ctttcgcagc gaatacatga gaatgcgtca tacagcgcag agaggaacgc agttcccata
     1561 cggctttgcg ggaatgaaca aacaacaccg caaaaatgtg tcgttccgcg aaacaaaaac
     1621 cctcttgcaa aacgacggag tgttcatcat aatagcaccc gtagtccccg aaaagtgaat
     1681 aggcttaccg gttcaaggcg ctgcggcgct gcgatgaaag atggaatgag ggtcagagtc
     1741 cccgcgaaca ggtcactgtg aggcctcccg atgtggagga taagtcaggt acacgccgga
     1801 aatgccacga atgcaagcgc ctgaaaccga gatgcatgaa cgggggcttc gcgcccgttt
     1861 acacgagaga agcgtcggca tttgccggcg ctttttgcat gaagtcaggg cacctatggg
     1921 ggacggaaag gtgcttgaaa aaatgggaga acgcaggaaa tacgtccgca gggacgccac
     1981 ggtggaacgg ttgtccgcta tctttctgga ggacatggag gggagcgtaa agccctccac
     2041 gctggcgtgc taccgccgca acatccaatg ccacatcctg tcggcgctgg gagcgcgcgt
     2101 ggcggcggaa ctgacggcgg cggagatcaa cgactacatc cagcagttac aggaggacta
     2161 ttcgcccaag ctggtgcgag aaatcggtgg ccttctgctg cggatcgtag gtatggccgg
     2221 tgtgggctac ggcgaggatg tgacgctgcc gaaggtacgg cagaaggccg tggaggtatt
     2281 caccgagccg gaactgaagc agatggggca ggtcattctg cgccgtccgg acaggacggg
     2341 acttggcgtg ctgctgacag cctacaccgg gttgcggctg ggagagctgt gcggcctgca
     2401 atggcaggac gtggacgtcg gcgcgggact gctgcacatc cagcggacgg tggagcgtat
     2461 cgcgcagata ggcggcggga cgtgcctgac ggtgcagcca ccgaaaacgg aaaattccga
     2521 acgctggata cccattccga aagagatgct gcggatgctg aagccagaga caaagcggcc
     2581 ggacagctac ctgctgaccg gcggggagat cgtccctgac ccgcgggcga tgcaataccg
     2641 ctataaggtg ttgttggagc gctgcggcgt gcggtatcgg aatttccatt g
//
LOCUS       DAWAEA010000182         2679 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5575_length_2679_cov_2.198171, whole genome shotgun sequence.
ACCESSION   DAWAEA010000182 DAWAEA010000000
VERSION     DAWAEA010000182.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5575_length_2679_cov_2.198171"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1943..1952
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          548 a          910 c          718 g          493 t
ORIGIN      
        1 ggcttgaatt gcagggcccg aaccacgatc actgccagcc agagcaccag cagccccaga
       61 aggatccata ccagcgtcat ttgtttccac tctccttttc cgttccgatc atgcctttcc
      121 gatacagaag ccgcgccgcg ccgatggcga tcagcgcgcc cacaggcacc agcacgctga
      181 cgctgctgct caggctgggc accagcagga acagcaccac catgaacagc atgggagccg
      241 ccgcgatctt cgggttcttg gatacgaaca ccacgcccag cgcgccgaac agggacggca
      301 aaatgttgtc aaaggcgggc ttcagcaccg gggattccag caccggctcc agcagccgca
      361 gaccgaacac gccgatggcg atcaccacca ccgtcacaat agagctggac gcaatggcga
      421 tggtagagat cacctcgccc tcctccgtcc cggccttgac cttggccgcc tgcatggcgt
      481 tcagggcgca gggcactttc aggttggtca gattgcccgt cacgaagccc agataggttc
      541 cgccggtgcc cagcatgggc acgtaggtca gcacctcgat aatgcccacc agccagtaca
      601 tgggcgctac gcccagcagg gccttcagca ccgccgtcag cggcggccac gcattgtaat
      661 acacgcagat ggccagtgga acggccagca tcatcataag aagcagcgcc gtccagacgc
      721 gcccgtaggt atattgtttc tcctcatagg gagacagctg cttgtccatt ctctttccca
      781 ccctcttaag ccatcagcgc cgtaatggga atgctcagcg ccatcgcgcc caacatgcac
      841 aggggcaggg cgtactgctc cagccacgtc catttcagca cccggatcag aatgccgcac
      901 acgcccatca gcagcgccga gaccagcgcc accacgatgg ggataaagcc cggcacgccg
      961 tcccgcacat tggcgaacag aagccccaga aacgcggaaa tcatccccag aaacagcgcg
     1021 tccatcaaga tcccgcgcca cttttcatcc ttgccgccca ggctgttcat gcccttctgc
     1081 agctttttca gtccgaacag gatcaggacg atgcccgcaa taatgcccag cgtcatcgtc
     1141 cacgcgatca ctgcgaaggt ctgggcgctg gtaatggttt ccgtcacccc ggcaccgacg
     1201 gcgttggcgg cgatggtggc tgcgggcagt tcgtaggtca cagcgcccag aatactcagc
     1261 cgcagccacg ggaagggcag gccgagaaaa cgattcagcg tcagtacgcc gatcaggatg
     1321 gacacggccg gtgccagcga gaacaatccg ctggacagga tcgtcttgcg aatggtcccc
     1381 ttggacaggt tcagctcccg ggcgcgccgc acggccttca ccagaaagaa gaccgattcc
     1441 gccaacaccg cagcgatcac gatgccggac aaccagaaca gaaacgggtc attgacggag
     1501 aacggcatat tctcccacct cccaggataa gttcaggatc aatacttccg cattgtagca
     1561 ttcctgcaag tccgcgtcaa tggcgggttt cagcgttcaa tggtgtcgtc ctcgaagcat
     1621 tcccgcatca gcccggagcg gcccatcact ctcccctgtc cttccaggtg ggacgcgtcg
     1681 cccagcccgc agcagcggaa gatgatcttt cccttggtcc tttcctccgt taccagcgtc
     1741 gtcaccgaag cggtgggcat aaagaacgcc tgccacaaca ccgccggaga gatcatcatc
     1801 agatgggaca gaatcgccat gctgatgccg aaatggcaga acagggcgat ggttttgtga
     1861 ttgttttcgc ctgtatacag gtttccctga cgaatcagcc cgtagcgggc caacagctcg
     1921 tccaccccga cggcagtttc gtnnnnnnnn nncccatcct cccgccactg ggtgggatgc
     1981 agcagcttct cctgcgccgt ccagtactgg ggcatcaggt tccacggaca gcggcgcatg
     2041 ccggtgtacg gctcgtcgaa cccgccccag aactccctca accacggcag tgtctcgccg
     2101 gtgcgcccca gcgctttcag cgtgacgccg gcggtgtcct gcgcccgtcc cagcggcgaa
     2161 agataaaagt cgtcaatagc ggccgactgc agccgccggg ccagcagttc cgcctccacc
     2221 cagccctgct ccgtcagcgt gtcgtgctca taatcggggt ctccgtgccg gatcagcaaa
     2281 attttcattg ttctttcccc tttccgctgc cttgcatttt taccactata tcacattttt
     2341 cccgcgccac caactttttt cggccatgga aaaaccctcc tgcgaacttt tccgtccgca
     2401 ggagggtctc tttcctgatt tcttttccgt caggcgtcga tggaatcgtg gaactgcatc
     2461 ggcaccttcc gtccatacac ccgcatggag cgcatggcca tgcccggcag gctcttcagg
     2521 atgcgcaccc ggaaggcgta gccccgcatc ttgcccagcg ggatgatccg cttgtggccg
     2581 atgctctgtc cctgagccag cggcacccag cgatccagtc cgtccttggt gcaccagtag
     2641 gcgggccagc gggtctccac cacccactcc agcacatgc
//
LOCUS       DAWAEA010000183         2673 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5589_length_2673_cov_1.961039, whole genome shotgun sequence.
ACCESSION   DAWAEA010000183 DAWAEA010000000
VERSION     DAWAEA010000183.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5589_length_2673_cov_1.961039"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          568 a          794 c          747 g          564 t
ORIGIN      
        1 gtacaccggc gatggccgcc agcgcagagc cgatggcgaa ggtcacggag atagtgcggt
       61 tcacgttcac gcccatcagc tgggccgcgc ccttatcctc gctgacggcg cgcatggcct
      121 tgcccatacg ggtcttgccg gtgaaccatg tcaaagccag catgatcaga atgcaggcca
      181 gcaccgtcac aatggccacg tcagaaataa tcagctggcc atcaaacaga cgcaccggtc
      241 cgcagaaggt agctcatgcc gatggccgta atcagcaccg ccagcgaggg cgcgcttcga
      301 aggggcttgt acgccagccg ttcgatcgtc atacccagca gcgtgcagac gaacatggct
      361 gccagcacgg acagccacgc aggcagcttc aggtattggg taacgcagaa ggaaatgtaa
      421 gcgccgatca tgatgacgtc gccgtgggcg aagttcagca tcttggcgat gccgtacacc
      481 atagtgtaac ccagtgcgat gatcgcgtac acacttccca gactgatacc gttgatcagg
      541 ttggaaagga acgacaagtg catcaacccc caatctctct tgtggaaaaa ggaaccgttt
      601 ctattgtaat tccaaacatt caaaacaaca agcggcaaaa tgtccgtctc tgttcttttc
      661 ccgttttatt ttccgatata taggcaaatg gagagagccg ctcatgcagc gttctccatt
      721 tgccgtcaga acgtttccgt ctgctcagaa ggcgggcgag ttcaccgccc ttccttctca
      781 ccgtccaaat atcggacggc aatgggcccg ttactgggct tcttccacgt tgtcagcgcc
      841 cacgtacgcg ccgtccttga taacggcggc gtaaggagtc ttgctcactt cgcccgcggc
      901 gttccaagtc atgacgccgg tcacgccgtc cacggtgacg gtgggcatat aggcgatcat
      961 ggcttcgcaa atctcctcgg cgctcatgtc agcgttgatc gcgccgtcct tcagagcctg
     1021 atagatcacg tatacacagt cataggtatc ggcggagaac tgagtgggga tctcgccgta
     1081 cttttcttcg tacttggcgg tgaaggccgc gctcttttct tccttggagt ccgcagagta
     1141 gggggtcagc agcataatgc cctcggccag actggtgtca aagccttcca tggtcaaaag
     1201 gccgtccatg ccgtccacgc cgaagaacac gggcttgtat tccaccgcag cggcctgctt
     1261 gaagatcata gaagcagggg tgtagtagat gggcaggaac accagctcag cgccgttgtc
     1321 cttcgcgtcg gccacctgca cggagaagtc ggtggtgtca tcggtgaagg tggtcacgga
     1381 gacgatctcc aggcccagct ccttggcctt ggcctcgaag gtctgataaa tgccggtgga
     1441 gtacgcgtca gcgttattgt agatcacggc gaccttgctg gccagcttat gctcggcgat
     1501 gtactcagca gaagcggagc cctgcgccgg gtcggagaag cagacctgga acatattgtc
     1561 cttatcggcg gtcacgctgg gggaggaggc ggagggggtc agcatgaaaa cgcgctcgtc
     1621 atacgccaca gcggaaacgg ctacgcaggg cgtggtggtc acgcagccga tgatcagctg
     1681 agcgccctta tccatcaggc tgttatacgc attgacggac ttttcggcgt catgttcgtc
     1741 gtcctcggcc ataaactcga tctgagggcc gcccaacgcg ttcacctcgt tcacggcaat
     1801 ttctccaccc tgttttgcag aagtgccata tacggcagct gcgcccgtca ggggtccaat
     1861 gaaaccaatc ttcagtgcgc cctcagccat cgcgggcagg gcgaagcata ctaccatcat
     1921 cattgcaact accatagtga ccagtttctt cattgtcttt tcctccttct aaacttccta
     1981 tccttcttac ctgtccggga gggaggctct ctgacaggat tggtttatta tagccacgtc
     2041 cgccccccca tgcaagcatt ttccgcccac tttcctgttt ttgtattgtt tttttcaaac
     2101 cccggccaac attccaccat tcccgccatt cacaaggggt agtacccctt gaccccggac
     2161 tgcgcggcag ggggtgaccc cctgcacccc acaccgcgcc catagggcgc gggggtactc
     2221 gagggaaagc agattgactg cggagttacc gccgctcggg ggctgagccc ctttcggact
     2281 gcgcggcgga gccgcacagg ggcgtaggga gacatgcggc ttggctgcgg agttaccgcc
     2341 gctcggcgac gggcctcgcg gctcgcgtgt ctggcagggg tagtacccct gcaccccgaa
     2401 ccgccgtcgg gcgacaggcc ctccggctcg ctaaaaatgc cagcgtggat atgaaatcat
     2461 actggtcttt tacgttgcaa aaatctccaa accgcatcga agcatcccaa gtggtgatac
     2521 cattaagtat gtttcacgtc aaacgtggaa atataaccca gcctgttttc tgcgctgcaa
     2581 aaacgtctca gctgcatcga aacatcccaa gcaacgccat ttgagtctgc ccagcgccaa
     2641 ctgcggggga gcagagcggc gataggcagc gga
//
LOCUS       DAWAEA010000184         2659 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5616_length_2659_cov_2.368280, whole genome shotgun sequence.
ACCESSION   DAWAEA010000184 DAWAEA010000000
VERSION     DAWAEA010000184.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5616_length_2659_cov_2.368280"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1873..1882
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          443 a          805 c          858 g          543 t
ORIGIN      
        1 ccagaccggc gagccgtggc acctgtcgtg ccacggcggt aaaaccccgg ccagctcgca
       61 tatcccacga gcaccctgcg cggcatagcc gcgcagagat gggtgcaggg ctttagccct
      121 gcctgcggta gtcggggagg agcttggggg agacggcagt ggtgtcgatg cccgcgtcgg
      181 cgcattcctt ttcgaaggcg gcgatgtgag caagggtcag cttgccgacg gtgacttcct
      241 tggcgtgggc ggcggcgttc tgaccggcgc tgcggccaaa gacgatgata tccagaaggg
      301 agttgcccat caggcggttc cgaccgtgga tgccgccgac ggcctcgccc gccacgtaaa
      361 ggttctccac gttggtggtc atgccgttaa cgttgatgtc cagaccgccg ttttggtagt
      421 gcagggtcgg gtagaccaga atgggctcct tgcggatgtc gatgccgtac tgggcaaaca
      481 tgcgcatcat ggcgggaatg cgggcctcga tggtgccttc gccgccgatc atttcgatca
      541 tgggggtgtc cagccagacg gcttcgccgc tgccggtgtc cacaccctcg tgccggtcag
      601 agcactcgcg gatgatggaa gcagcggcca cgtcgcgggt ctccagcgga tgcatgaaga
      661 ccttgccgtc ccggttgacc agcttcgcgc ccagagaacg gactttctcc gtcaccaacg
      721 cgccgaaaat ctgctggggg aaggccgcgc cggtgggatg atactgcagg gtgtcggcat
      781 agagcagctt cgcgcccacg cggtagccca gcaccagacc gtcggcggta gcgccgtagt
      841 ggttggaggt ggggaagccc tgatagtgca tccggcccgc gccgccggtg gcgatgatga
      901 cggtcttggc ccgggccacc atcagttctt tggtttccat gttcatcagg acggcaccgg
      961 cagcacggcc gttttcgtcc agaatcagct cgatagccgc ggtgaagtcc accaccggga
     1021 tgccacggtt cagcacttcg tcccgcaggg tgcgcatgat ctccgcgccg gaatagtcct
     1081 tcgccgcgtg catacgcttg cgggaggtgc cgccgccgtg ggtggtgatc atggtgccgt
     1141 cgggagcctt gtcaaactcc acgcccagat tgttcagcca ctgaatggcc tcgggcgcgt
     1201 cgcacaccag cttggacagc agttcccgct tggcggcaaa gtgtccgccg ccgaaagcgt
     1261 ccaggaagtg aatggcaggg gaatcgttgg gcttgtccgc cgcctgaatg ccgccctcgg
     1321 ccatcatggt gttggcgtcg ccgatgcgca gcttggtgac gatcataacg ttcgcgcccg
     1381 cattgtcggc ctcgatggca gcggaagcgc ccgcgccgcc gccgccgata accagcacgt
     1441 ccacatcgta gcgcacgtca ttcagatcca cgtcgtcagc ggaaatgcgg ctgtgagact
     1501 gcagcagctc gcacagctcg tgcggcacgc tctcgccctt gttgggaccc accttcaggg
     1561 tggcaaactc gctctttttg tagtccggat ggtaggcggc cagcaggtcg tccttctcct
     1621 gggcggtcat gcgggcgggc tcataggcca cgttggccgc gcgggccgct tccacagcct
     1681 tcagggattc ttgaaactgt tcggaataca tgcggctccc tccttacttt tcaatctcgc
     1741 ggtggttgta cagctctttg agttcttcca ccggcttgcc catgagggct tcgatcaggt
     1801 cggtgaaatc gccgttcttg atctcctgca cccggtcttc cagatgctgg ctcttagggg
     1861 ccagatactt gcnnnnnnnn nnatacttgc cgttcaggcg gcgggccagc atggccacct
     1921 ggggatggga gatgccggcg gggcagcggg aggagcacac gccgcacatg acgcagtcga
     1981 agctctcctc ggcgcacttg tcaaattcgc cgcgctgggc gtaggcgatg tactgcatga
     2041 cgttcagctt ctgagtgcag ctcttggtgc aggcgttgca gcccacgcag gcgtaaattt
     2101 cggggtagag ctgcatcatg atctgctcgg tgggcttgat cttttcgatg tcgtagacct
     2161 gcttcaccag cgggaagaag ggcagggtcg ccacgtacat attgtcttct accttggtct
     2221 ggcaggccag acaggtcttc agctcccggt cgcccttgat gcggtagatg gtggcgcagg
     2281 cgccgcagaa gccgttgcgg cagccgcagc cgcgcaccag ctggtagccc gcgtattcca
     2341 tcgcccgcat gatggtcagg tcgtccggaa cctgatactg cttgccaaac aggaaaatat
     2401 tgaccatgtt tgccatgttc ttgatctcct tctcaatact cgttgggcag ttcgtccagc
     2461 tggtcgcagc ggaacacggg gccgtccttg cagacgtact tcgcgcccac gttgcagcgg
     2521 ccgcacttgc cgatgccgca cttcatgcgc agctcgaggg tggtgtagac ctgcgtcttg
     2581 tcaaagccca gctcgatcag gcccgcaagg gtgaacttga tcatgatggg cgggccgcag
     2641 aggatggcca ccttgtttg
//
LOCUS       DAWAEA010000185         2626 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5691_length_2626_cov_1.886037, whole genome shotgun sequence.
ACCESSION   DAWAEA010000185 DAWAEA010000000
VERSION     DAWAEA010000185.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5691_length_2626_cov_1.886037"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2024..2039
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          478 a          848 c          719 g          565 t
ORIGIN      
        1 atcagttttt ccacgccgct taaaaacagc gcttcatact cgctgtggta gcaccccaca
       61 taatcccgct ggaagggcac ccgccaccag ccgccctcca tcgcgccttt ttcgtctacc
      121 accttcactt tgtcctgcag ctcgctccat tggggagcca tggtggagag ctcatagccg
      181 aaatagggca gtaccttgat gccccgccgg tggcattcat ctacgattgt ccgcaactgg
      241 tgagcggtaa attcgctcag ttcgaaccag ttctggcact tgttccattt ttcgtgcagg
      301 atgagcgtgg taacaccctt ttccttcagc tcgtcatagc gtcccgccag cacgtcgata
      361 tagttgccct tgaccttggc gccgcagtcg atatggaacg cattgtgaat gtacggctgt
      421 ttggggaagg gctttaccgg cgtggcctgc aggccgaagc agaacgtgac gggatgatag
      481 gcgtatacgc cgtccgcagg gtcagccgtc cacgttttgg gctggctgtc cagcaggcgg
      541 acgcgcagga tcagctgttt gccatcccgc accagctcca tggccctgtt cgggtcttcc
      601 ggctgccagt ttcgggcgct ttccgcatac catccaaggc cccgttcctc attgcccagc
      661 cacagcaggg ccttgaaggg catggacgcg ttctgcgcgg ggacggcccc gctggcggac
      721 atggtcccca tgggccgctc tgttccgtca gccagcgaga tcacgctgtt tgggaacata
      781 tggaacagcg tggccgcctc cgcccgcagg ggaatttcca gccacagccg gtccagccgg
      841 aaccgcatgg gcttggcgtc cgccacgccg aagacctgcg ccaccgtccg gccccggggc
      901 atcagcttca ggtcgatgtc cacgccgccg tcgtagccga tgcgggtgca ggtatccaca
      961 ataaagcgtt cggactgttt cgcgccgcat acgatgattt cctcgtcgct gcgccgctga
     1021 ataaagctct cggcctcgtt ttcggagtag ttgtcgtccc agtgggacgg ctccccgtcc
     1081 tccacggcta cgacccgcac aggcgcggcc agcagctcca cgccctgaga acgaatggag
     1141 cggagcattc cgtccgcgcc tacggtatag tcccggccaa tgacgtgaat cgtatgttcg
     1201 tagttctgac tttccgcccg cagcggcaga aacggctgag gaatcttcat ccggcattct
     1261 cctttttctt tcttgcgttt tatggggaat cagtggatcc gaaagtccag cgctttgggc
     1321 aaggggtatt tttcatccgc ggcacgaatc agcttctcgt cggcggggtc gtagggaacg
     1381 cccgtctctt tggcgtacag gcggcggtac agctcgaacc acagatagga aaccgacagc
     1441 tcgccctcct tcacatcggg catgacaaac gcccggttca ggatttcggc agcttcttcc
     1501 aaccggccga tgtgcagcag cgccacagcc cgcaaaagcc gcagccgtcc gtaggcctgt
     1561 aattccggcg aaagctcgtc ataaagctcc aaccactttt cgtcgctgcc gtcctccgtc
     1621 aactgcatgg ccgtttcaac ggccagcgcc cggcagtccg gcttcagccg aaacgcattc
     1681 agcagcattt cgtgcgcctg tttcggaatg tgatattcat tcttatacag catagccaga
     1741 ttccgcagtg cccacgggtt ttcgcagacg gccagcgact gctcccacgc tttcctcgct
     1801 ccgtccacgg aaccatctcc gtaggcggcc atcgcgtaac ggataactcc ctgctgcaat
     1861 aggctatacc agttctgctc aggcagcgcc tccagctttt ccagccattc ccggcccatg
     1921 acatagcctg ccggctcctc catggggtcg gggcaaggaa agtgtccctt ttccagcaaa
     1981 tcgagccaaa ccgcgctttc cgggtcgttt gtcttcggga aggnnnnnnn nnnnnnnnnc
     2041 ctcaccgccc ggacagcctc gccatactcg ccgtgcagca ctttcgggtc gcccttcagc
     2101 agcgtgtatg cctcctgcca ctcccacacg gtgcggcccg ccatggggat gtgttccagc
     2161 tgagtatgag ccagcccggc ctgaatttcg atataggcgg agcctttctc gctgagccac
     2221 tcgttccagt tccttcctcc ctgccccatg ccccatacaa acattttccg tccaaacagg
     2281 cggcgggtgg agcattgcag caggccatag ccctcttcat tggcggatgc gatccacttg
     2341 ggcgactcgt ccgggatctg gtaaaagaaa tcccgggatg aagggattcg agtaggatag
     2401 ctcacgtcca cgccatccgc cattggaatg gccgtcttgt ccagaatata gtgatcggcg
     2461 ttgtagaagc tcaaaaaaga ctgctgcgcc gggacgatca cccgggtgcc cggcgtttcc
     2521 ggcacggcga tattggacca ccagtacata tacttcgtct cgtccgtgtg gttctcgatc
     2581 cggcagcgca ggtacagcgt gggcgaatcc tcccgcaccc acgcgc
//
LOCUS       DAWAEA010000186         2614 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5719_length_2614_cov_1.325127, whole genome shotgun sequence.
ACCESSION   DAWAEA010000186 DAWAEA010000000
VERSION     DAWAEA010000186.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5719_length_2614_cov_1.325127"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    514..523
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1130..1263
                     /estimated_length=134
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2009..2018
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          442 a          735 c          738 g          545 t
ORIGIN      
        1 gcatcccgta gccgtagccc ttgtggctgc ccagcgtttc cgtgttgccg cccaaaggca
       61 tgatgccgcc gccgttcttg gcgacgatgt tcttcagcac gtctttcgca tcggtggaag
      121 gatgtccgtc cttgtccagc gcccagccct ccggcagggg cttgcccatc ttgttgtaca
      181 cttccagctt gccgcgggtg accacagtgg tggacgcgtc aaagaaaaag ggatagggct
      241 cggcgggcac cgccagcgcg atggggttgg agccgatcat ggccatacgg ccaaaggtgg
      301 gcaccatgat ggcctcgctg ttggtcatgg ccatgccgac cagcccctga tcgcaggcca
      361 ttttggcata gtaaccggca atgccgaaat gattggaatt acgaacggac acgatcgcca
      421 tgccgacttt tttcgccttt gcgatggcca gctccatggc ctgatggctc accagctgac
      481 ccatggcgtc gtgcgcgtcg attacggcag aaannnnnnn nnntcgtgga cgatctccgg
      541 cttggcatga acgtcgatca tacccttctc aatgcccttg tgatagcgca ccaggcgctg
      601 catcccgtgg ctttcgatgc cgtacaggtc ggacagaagc agtacgtcgc tgatgatccg
      661 ggcttcgctt tcgctgaagc cgaacttttg gaacgcatcc atgcagaagg tgttcagttg
      721 gtcgtaggaa tagtgcaggt actttgcggc cattggaatc ctctcctttg gggtgatgga
      781 atggagcgcc tttgtcaaaa ggcgcgggaa aacgagtacc ccgctaaggg gattcggaaa
      841 ccgggcaaag atttcctgcc ggagcccggc cccggcccgt ctttcgccca tttctttccg
      901 gcttctgaac gcagaggagg cttcccgcgc cttctatgag cttacagacg ttcggacagc
      961 atcacgaaca gatccctgta ttcatagccc tcgggcacag tggcgctgag cgcttcggtg
     1021 gactgggggc aggcggcgac catgatcttc gcgtcggtgc gggtgatgtc gttccagcgg
     1081 cccgcagcta ccttggcccg cagctcgggc aggtaacggc ctacgacggn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnatgcccc agtccttgta ctcatggcga aacagcgccg catcatagct gtccagcacg
     1321 acgaccttct tcgcgccgga ggccttgatg gccgcagcgg ctttcttggc ctgcacgcgc
     1381 acgtcctcca caaagcccat cagatcgccc agcgcggagc cggaggaagg ctcgttcttc
     1441 agcacggcgt acttcacacc ggccttgtcc aggatcttca gcagggcctc ggccacctcg
     1501 ggggtggaat aacgggccgt ttcgcccagc cataccagca gatcgccggt ttcgggcgcg
     1561 tcggtcctga ccttgctctc agacgcgccg tacatgttgc cggtcttctc gatcacgtcg
     1621 atcaagtccg tcacatactt gggggcaacg cccgccacca cggactcggt gcggccttcc
     1681 cggatgaaca gcgggggctc gaagccggtc acgcagtcgt tggtgcaggc tccgcacagc
     1741 atgcattcgt acatggtctc ggcggtatcg gcttccagct tcatgccgcg ggtaatcatg
     1801 ctcaccagca ggcccttggc ccggggagtg ttggtttcct tgccggtcac cagctgtacg
     1861 gggcagaggt ggcggcacat ccagcaaaag cggcacgcgt ccgcgtgttt cttggatttc
     1921 tcgctaaaca tcgtttctct ctccttatcg gttgccacta gaatcatgca aaaaatgaaa
     1981 cgtcaaagcc ttatggcgcg atagctgann nnnnnnnngg gtgcaggggc actgcccctg
     2041 ccagggcttc gccctggccc cattttacgt tctgggaggt cagcttctca agcgttcgcc
     2101 gtgtgcgggt tataggccca gcttgtaggg gttcatgatg ccgttggggt ccagcccctt
     2161 cttcaaactc tccatgacct gcatggcggg gccgtactgc tccttcatca gacgggacag
     2221 cttcaggccg atgccgtgat ggtcgttgat gacgccgccg ttctccatgg ccgcgcgcac
     2281 accagcgttc caaatctggt tgtgcagacg gatggcctcg tcggggtcgg agggcacgtg
     2341 ggcgggatcc acgatgaaac gggtgtagtt catgcagccc cactcgtacc agtggctgga
     2401 atgagagatg tacttcacat aggggaattt ttcgttgatg accttcttca tggccagata
     2461 aattttttca atgttggcat aggtggccac ggtgtccatg gtgccgaaca tctggggaat
     2521 atccatgatg ttgccggggt agaagaaggt gatgcggttc tcgtaccact tttcgccgta
     2581 ttcgccgccc agatcctgcg cgccccactt ggcg
//
LOCUS       DAWAEA010000187         2609 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5728_length_2609_cov_1.942835, whole genome shotgun sequence.
ACCESSION   DAWAEA010000187 DAWAEA010000000
VERSION     DAWAEA010000187.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5728_length_2609_cov_1.942835"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2129..2138
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          545 a          839 c          687 g          528 t
ORIGIN      
        1 ttcagcagtt cttccgtgtt gattcgcacc tttatgatca ccacgcgccg ggatagccca
       61 gcgttacggc tgcattttcc gctccggcac gcatgcaggc cgggatcgac tcctttttca
      121 gccacgccat caggaatccg gcaataaagc tgtcccccgc gcccatggta tccaccacgg
      181 agcacggaac gatctggccc ggatagaagc tttcgccgtc ataggcaagg ctcccttttc
      241 cgcctcgggt ggccaccacg atctgggggc ccagcgccgc tacctccaga atccgcgcct
      301 tgaggctttc ctcatcggag gcgtcatctg agaaaaacgc cacgttcaca tggggcagcg
      361 cgatcctgcc cgcctcatcc agcgggcgct cggccccgtc aaaggccacg gggatcccca
      421 tggcgcgaac gtcggccagt tgactttccg tatgccccca cagtccggtg accagcagat
      481 tgtatccctt gaggaagtcg aaatccgcct gccggagtct gaaatccgcc atgacgccct
      541 catcataatc gccgaatacc cgctcaccat tgatgcgctc tacatgggtg aaggcagttt
      601 tgccgggcag aatctgcaca tggctgacgt ctacgccctt ctcttgcagc gcggcgcaca
      661 tctgttttcc aaaatcatcc gtacccaccg cgcccagata ggcggactgg ccgcccagac
      721 gccttacata caccgaaaca ttgaccggat ttccgccggg aaagctctcg ccggtgttgt
      781 cataataatc cacacagttg tcgcctgcgc acgcaatttt tatcataata actcctttgc
      841 cctttgcagc gccagtcctg cggcgccggt aggatcgtcc atatagtgag tcaccagctc
      901 caacgtggcg cggccgtcat agcccgcctg ccggaagtcg ctgacaagct gtttcaggtt
      961 caatacaccg tctccgggga tcagatgggt ttccgtcacg ccatcgcagt ccaccagatg
     1021 caaatgggcc attctgcttc ccagatggcg gcaatagtcc accgggtcct cgccctgcaa
     1081 taccggcggc accacatcgc acatccccat cagccacggc gagtccaccg cctccagcag
     1141 ctccttgagc tcttgaagcc gtgtgcaggt gttgctctcc agaggggaca gcgtttccag
     1201 cagaaggaca acatgctccc gttcggcagc gcgggcaaga tgctccacag agcggcgcag
     1261 ccgtgcgcgg cgcaattccg ccgggcactc gccgccgtgt cccacggaaa tcagcatctg
     1321 cccggcgccg atcagcccgg cggcacggac agacacctcc agatacgcca tacaattgcg
     1381 ccactgcttt tccgtcccca gcatatagtt atagggatag gcattgtgct ccggcgtata
     1441 gacaggcacg ggaatgccgt aatgctcctg caagcggcgg atctcccgat agtcctctat
     1501 cacagccggg gcaaaggcgt ggggatagcc gccccaaagc tctatgtaat cataccccag
     1561 ttgcgcagcg tctgtaaagc atttttccag cggcagacgc tgataaccgc tggtaaacat
     1621 cccaaatttc ataagcaaac ctctgaaaaa agggcggggg aacccgccct ttttcacttc
     1681 atacgctaac ttccggtagt accggcgaat ggccatgtcg tggccgctga tggcttccac
     1741 atgggcgtcg atgcgattgg ttaccgcgtg cataatggca tagcacaccc gtccgcggta
     1801 cttctcatcg ataccctcca gcggataatc atgggtgtcg ataatggtga agttctcgca
     1861 aatctgcggc aggaacttcg ccacacgcag gcccaggggc cgctgggcat cctcgcccac
     1921 aaagaccgtg accggcgtat ccttgtccac gatttccagc ataccgtgga agaactcggc
     1981 actgtggatg gatttcgtcc gaatccagtg catctcttcc cagtagcaca tggcgtagga
     2041 ataggtggcg ccatacaggc agccggcgcc cacaaagtag tggagcttgt cctccagatg
     2101 cttgcgggca aagtcctccg caaagggann nnnnnnnnca atcttctcga tctccaccag
     2161 atccttggcc agataggcgt ccatctgggc ataataggtg tcatagtcgg ggaagttgcc
     2221 ctgacggcgc aggaagcagt ccgccaccat aaagaacttc agctgttcat tgcccttgta
     2281 gctgatgcag taatccacca gagtggccag ctcagcatcc ggcttgtcaa tgaaccccag
     2341 cacctttgcg ccgtggctct gggccagacg aaccgcatcc acaatctccg tggtggtgcc
     2401 ggtcacagag gagagaacca caagggttcc cgcgcccaca cgcttatcgc ctgtggtgca
     2461 atactctgcg gcgttggtgg cgaaggtatc catgtcggta tgctccttga gatagcatac
     2521 cacctgcata cagctggccc atgtgccgcc gatgcccagc cagcagaggt ttttcacgcc
     2581 ctctgccagc agacggacga tcagctctt
//
LOCUS       DAWAEA010000188         2596 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5760_length_2596_cov_2.144431, whole genome shotgun sequence.
ACCESSION   DAWAEA010000188 DAWAEA010000000
VERSION     DAWAEA010000188.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5760_length_2596_cov_2.144431"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          552 a          780 c          769 g          495 t
ORIGIN      
        1 gtgctataat atgcttgagt gcgtctgaac tttttttcgg cgcgactcgc tttgccccgg
       61 atgggttatc ctattgctga aggaaacata ccgctgtcat caggagatgg aatatgaacg
      121 gaaatcgcat gaaatattca tccacgccca cctatgggat gcagaaacgc tcgccgctgg
      181 gcaaaaagcc cctgtttcaa ccaaagagcg ttatgggtcc cgattttgcc cgggcgaccc
      241 agcagtctac cgccgcgcca gccgccgact tttttaccgc gccggtgcag cagcccggag
      301 tgccggtaca gccggtcaat cccatgcagg cggggagcgt tccctatgcg gccgcgccgt
      361 atcagcagcc ctttgcgggc ggctatccca tgggcggaat gaccccggga gccatcccgg
      421 cggcgaatcc gtggatgacg gcttccccca tgatgcccca gcagggcagc ggaaatcttc
      481 cgccgctggg caacacggtc aaccctttgc aaagcagcgg gtttggcgtt cgctcccagg
      541 gctttgtgcc gccccagacg gcggccgcgc ccaatgtgca gcccgcagcg ccggggccga
      601 tgggatatgc gccccaaacg cccctgcagc ccatgaatcc catgacgaac gtgggctata
      661 tgcagtcggg cgtgcccttt ctgaatcagg gaagcatgaa ttacgccccg gtgaatccgg
      721 ccacgatgca gcaaaacgcc gcgccgcaag ccggtttcat gccgggaacg ccccagaata
      781 tccctatgca gggcggcgtt cccctgcccg gcagcgccca gcagggcggc catgccccga
      841 agcagcgcaa gccgctggat cttgacctgc tgttcggcat tttcctgttt gggctgcttc
      901 cgcttctgtt tgtgccctgc atttttgttt ccagcagtct gaacatcctg cgctacctgt
      961 tcatcggcct gagcgtggcc gggctgggcg tggtctggta ccggcagatg tactcttcca
     1021 cgattcgggt ggtgctgtcc gtgatcttcg tgtcgctgag catcatcgct ctgtccctgt
     1081 ccatgcaggg cggacgggat ctgcgtcaga cggctgctgg cagcgcagtg cccgccagtg
     1141 cccaaagcac ccttgccccc gatgaaagcg cggttcctgc ggcggcggag atcaatccta
     1201 cgcccagccc cgaacccacc ccggtgagca agccggaagt ccagcagcgt ctggaaacct
     1261 tcatgaacaa ctgggcgggc aatcagactg aagccatggc cagccttgtg cagcccagct
     1321 gggcttccca gcaggaaaac gctgcggccc agcttttcca gacggtgttg aataaccgca
     1381 ctcccctgag ctacaccatt gaggaagtgg gcggcacgga tgacgacacc agccgcagca
     1441 tcatcatgac cgccaccatc gacaagcaca acggcaagga tcccaagacc taccgcttca
     1501 atgtgctgat ggtcaaagag ggcaaccagt ggtatgtgga tcccaattct ctggccagca
     1561 acgacgagca gcagaccgaa gacgtggtgg tcaactccaa ggacaacgtg acgcagaccg
     1621 aggctccccg cactacggtc agccccgcac ctcccgcaag tacgctactg tattacgtac
     1681 accaattcgt taagcccctg tctggtatgc ggcgcgccca catcgacgct gcctacggac
     1741 gcccccgctg aataacacag aaggaacggt ctgaccatgg aaaaaaccaa tgtaatgcgt
     1801 cttttggacg cagccaagat cccttacggt tcctacgaat acgccgcgcc tgatggagcc
     1861 ctcagcggcg tggaggtggc ccgccagctg gggcaggaag aagaacgggt cttcaaaacg
     1921 ctggtgacgc aggcagcctc ggggaaatat tatgtatttg tcattcccgt gggagaagaa
     1981 ctgaatttga aaaaggcggc ctccgccgtc cacgagaaga agatcgagat gatcaagagt
     2041 aaggagcttc tgcccttgac cggctacatt cacggcggct gttcgcccat cggtatgaag
     2101 aagccgttcc ctacggtggt ggacgaaacg gcgcagcttt gggacacgat tattttcagc
     2161 gctgggaaaa tcggctatca ggtggaggta tccccggaga atttaggaaa gatgattcct
     2221 tacacctttg cggatgtgac gcaggggtaa ggggaagtct ttccaaaaga aaatgaaaaa
     2281 actgccgctt ctgaagaaat tcaggagcgg cggtttttat atggccggaa gttcgggcaa
     2341 acggccagcg gagcgcatgg agtgagggcc gcgccgattg gctgccccga ggaaagtccc
     2401 ctgcccgtga cggtgtatgc caactttgcc gtaaaagcaa gggattaagg gatgcggttt
     2461 ccatggttat ctcgccagct ttggtgcgtc cgcaggcaca tacccattgc cccgataggg
     2521 ctcgacgcgc cgactccggc gcgggtggtg gggctgccgc cccgaggggg gcaggacttt
     2581 ctttcgaagc gaaaga
//
LOCUS       DAWAEA010000189         2576 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5807_length_2576_cov_2.335581, whole genome shotgun sequence.
ACCESSION   DAWAEA010000189 DAWAEA010000000
VERSION     DAWAEA010000189.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5807_length_2576_cov_2.335581"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1998..2007
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          582 a          724 c          821 g          439 t
ORIGIN      
        1 cttctattcc tatccctaca ccagtgagcg agccaaggaa aaggagctgg atctggcgcg
       61 gatcctgtcc gagagcctgt gcggcatccg ggtgcatgtg gtgcccttta cggacattca
      121 gatgcagatc cacgaaaagt gccacgagga ctacaccacc atcatcatgc gccgctacat
      181 gatgcgcatt gccgaggcca ttgcgaagaa agaacacgcg caggggctga tcaccggcga
      241 gagcatcggt caggtagcca gtcagaccat gtacgccctc tgctgcaccg acgaggtggt
      301 ggatatgcct gtgttccggc cgctgatcgg ctttgacaag caggaaatca tcgacatcgc
      361 tgagaagatc ggcactatgg aaacgtcttc cttgcccttt gaggactgct gcactgtctt
      421 tacgccccgg caccctgcca ccaagcccaa gatggagatc attctggacg gggaaagcaa
      481 gctggatacg gaggccctga ttcaggcggc cattgacaac gaagaagtgt tcgagctgta
      541 agtcagaagc tcccgccaaa aaaacaggcc agcagcgcgc cgggcaggcc cagaaggccg
      601 gtcagcgccg aagcggcggg gcccgtaggg atagaaattc ccatgggaag gaagattcgg
      661 ctcagcagga agcatagaat gatgccaagg aacagccgct ccaccagaag aagcagccct
      721 ctgcgcgggg tggagcggag gctccagaac cacaaagcgg ccaaaatcag ggtaaaaacg
      781 ccgaggatcc acagacggac ggccatggac gcacgcctcc tgtcagtcag agaatggtac
      841 aggctatgcc cggctggctg ggaagatgcg gaaacccctg cttttttggc cagaactacc
      901 gaaatacgaa agaattgtca aaatcccttg cgattcccga acatttcctg tataatgccc
      961 gtgggataaa ttttgtcatg cgggacaaaa tttgtcccga cacgatgctt gggaaaaggt
     1021 ggcgagggga cgatgcagga taattatttt accctgattg aacggctcgc ccccgacttg
     1081 gcgcaggaga tcgagcgccg ggcgctggtg ctggaacgca ttggcgcgct gcagcctgtg
     1141 ggccgccgcc agctggcggc aaggctgaac ctgccggagc gggaagtccg cacggtggcg
     1201 acgctgctca aggagcacgg gctggtcagt ctggacgccg ccggaatgag cctgacgcag
     1261 gaagccagcg acattctccc catggcgcgg ctgttcagcc gggagctgcg gggcctgacc
     1321 aagctggaga ccgcgctttc cgctcgctgg aacggcacga aggtctgcgt ggttcccggg
     1381 gatgcggatc aggacgaaca tgtgcttcac gaagtggggc gctcagcggc cgcgcggctg
     1441 cggagcttcc tgcagagcgg ctccacgctg gcagtgaccg gcggcacgac catcggtcag
     1501 gtggcggcga cgctgcccgg cggtacgccc atgaacgtca tggtggttcc tgcccggggc
     1561 ggtattggcc gctcgctgga aacgcaggcc aacaccatcg cctgcgagat cgcccggcgg
     1621 ctgggcggtc atcaccgtat gatgcatctg cctgacctgc tggatgagca ggccctgaac
     1681 gagatgcgca agctcaagga aatcgacgaa acgctggcgc tgctggaacg ggccgacgta
     1741 gtgctgcacg gcatcggccg ggcagacgaa atggcccgca agcggcagct tcctcccgcc
     1801 gtggaggcgg aggtgctcaa acggggcgcg gtgtccgagg cctacggctg ctatttcgac
     1861 cgaaacggta aaatggtatg ctctgcctcc accgtggctc acgatctggg caagctgaaa
     1921 cccgactgcg cgtggatcgc cgtagcggcg ggcacgggca aggcggaggc catcgacggg
     1981 gctatgcggg cgaagccnnn nnnnnnnggt aacggacgag ggcgcggcca aggcgattct
     2041 ggcgctgccc gaaaaggaat gtttgcacgc ctgaaaaacg gctttgcaat agaaattttc
     2101 atctgctaag gagtgttggt tatggcgaaa aagaccattg aagacattca ggtcaacgga
     2161 aaaaaggtac tggtacgctg tgatttcaac gtgcccatgg acgaaaacaa agtgatcacc
     2221 gatgagaacc gcctccgcgg cgcgcttccc accattcagt atctgatcaa gaatggcgcg
     2281 aaagtcattc tgtgctctca tctgggccgt cccaagggag agttcaacat gaagtattct
     2341 ctgcgccccg tcgccgagcg tctgagccag ctgctgggtc aggaagtgaa gatggcggac
     2401 gacgtgatcg gcgaatccgc ccacaagctg gcctccgaac tgaaggacgg cgaagtgatg
     2461 ctgctggaga atacccgctt tgagaagggc gagaccaaga acgatcccga gctgagcaag
     2521 gctctggctt ccatggctga tctctttgtg aacgatgcct tcggcaccgc ccaccg
//
LOCUS       DAWAEA010000190         2576 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5809_length_2576_cov_2.201111, whole genome shotgun sequence.
ACCESSION   DAWAEA010000190 DAWAEA010000000
VERSION     DAWAEA010000190.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5809_length_2576_cov_2.201111"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          464 a          799 c          800 g          513 t
ORIGIN      
        1 ggcggagatc tcccgcacgt ttttcagctg catcttgtgg tagcggtcca gcatttccgc
       61 cacggcccag ctcagcgcgc cagcggcctt gtgggggtcg ctcaccacgg ggatcagcag
      121 atgtggaatc acattgtagc actgcaattc caccactttg ggatcgatca tgatcatgcg
      181 cacgtcttcg gggctggtgc ggtacagaag gctgttgatg atggcgttga tgcagacgga
      241 tttaccgctg ccggtctgac cggcaatcag caggtggggc atcttggcaa tgtcgcacag
      301 caccggcttg cccgcgatgt cccggcccag cgccaccgtc aggggcgact tggcgctttt
      361 catttccggg cttaagagca cctcagccag cgaaacggac tgaatttccc ggttgggcac
      421 ctcgatgccg aacaggttgg tgccggggat ggggatctcg atccgaacgc cgccgttggc
      481 cgccagatcc agcgcgatat tgtccgcgat gctcatgatg cgcttgacgt tcacgccgct
      541 ggacagaaga cccagctcga accgggtcac ggcggggcca tgagtgactc gctgcacctt
      601 ggcgggaata ttgaagcttt ccagcgtttt ttccagccgg ccggcgtttt cagtgtcctg
      661 cgcacgagtg tccttgatct cccgctgctg caggttcaaa agctcaatgg aggggtacac
      721 atagggcgtt tgccgccggg gctcatcgtg aatttccgtc accggggcgg tacgggtcac
      781 agggccggtc tgctgggcga tggaggtcgg gcgcttgtag gctgcatccc gatccacggg
      841 ttccgccgca gcaggggccg ttttcacagg ctgggccgcg gcagcatcca gcgtccggga
      901 agccgccgca ggggcgaagg tctgcatgga agcagcagac gcgccgggat tggacgaagc
      961 gggcgcgaag gtctgggaag ccgccgcggg agcgaacgtt tgagccgccg caggcgcaaa
     1021 attcggggca gaagcggcgt tttgcaaagg cgcggcagga gcggctgttt cctcccacgg
     1081 gggcgtttcc ccgctgcggg tggcttcctc gttcccggcc aattccgcgt cgatctccgc
     1141 ctttcgcttg cgaatctgtt ccagccgatc cagcgcccgc cgcttggagg aagacagaat
     1201 gaccgcgtct ccttccggct cctgcacagg ctccggctgt tcaggcacgg tctgggcgga
     1261 cggataggac ggcggcgcgc cgaaggggga acggttctgc ggggtcttgg ccttcttgcg
     1321 gcccttgcgc caaagaggct ccgcatcggc gggcatgacg atctcgttat acatttccgg
     1381 ctgctggggc tgctgcacct gctgccacgg ctggggagtc accggcgcgt aatagggctg
     1441 accgttgggg gcgtacatca gcgtatatcc cggaggcacg gcctgctgag cctgggccgc
     1501 catctgctgc tggtacatct gctgaggctg ctggggctga tcgtccagcc gttccgcctt
     1561 ctggggcttg cgccgccgca gaaacgcggg atagttcaag ctgttctgct cctcggcggc
     1621 ctgctgctgc gcttgccact gctgctgggc gtactgctcc tgcgcctgct ggacttctcg
     1681 tttctgcttc cgttcgccgt tcttttcctt gatggtctcg atcatctgca ggaagtcgaa
     1741 atgggtcatc agcagcacac aggcgatgca gatcaccccc agcaccgcca cgccaaaggt
     1801 cttgcccaga aacgtccagg cgggccacgc cagcagcatc cccagcgcgc cgccgaacac
     1861 gccgctgccg gaggaaattc ggtatccgaa gcggatcagg ttccagtagc cgtccgcctg
     1921 tgccagcggg gggctgtatt gggtctgatt gtactgcacg ccatagtcca tcagcccttg
     1981 cgtgccgacg gaactgagca gattgaaaat gcccagcacc gccagaaata acaccagcat
     2041 ggcgaaatag ctgcgctttg gcacattttt cccggcagtg aaaatgcaca gcgcgccgcc
     2101 ccagatgagc agcacgctga tgccgatgca cagactgccg ccaagcccct gcatcacctg
     2161 tttgatcttt tcaaaggcca tgccctgaac gccgcccact acggccagca gactgacaac
     2221 gcccaaaatg ctcataaaaa cgccgagtcc ggcaaaaaga gccagggtac ccggctcata
     2281 agctttttgt tttccgtttt gattggccat gaatcgatgg ttcctccatg ctcagcacag
     2341 ttgaatacag acaagaacgc ccgtttcatc cgcgtacagg cgcagttcat gttccccgaa
     2401 gcggtagacg tcgcagttcc cggcggaaag ctgataatca taagccatgc tttccgtcat
     2461 gacgatcact tccgccggtt cgcccaagga ggactgccac tccgtgcgga ggctcgtccc
     2521 caccttcagg ccgtacagag agccccggcg aagctgaacg ccccgaagct gcgaat
//
LOCUS       DAWAEA010000191         2574 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5814_length_2574_cov_1.563319, whole genome shotgun sequence.
ACCESSION   DAWAEA010000191 DAWAEA010000000
VERSION     DAWAEA010000191.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5814_length_2574_cov_1.563319"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          544 a          790 c          660 g          580 t
ORIGIN      
        1 ctccacttct tcgcggccgc cgccggactg catttccttc aggcagctca ggcaaacgtc
       61 cttgccgggc agcgcggggg ccgcgttgca tacggagcaa agttccatat cgtcattggt
      121 aggctcgccc ttcatctcgc gaaggcatac cttgcagtat ttttcatcct cccggatata
      181 attcagatcg caccgtgggc atttgaccag tttcatgtga tttttccctc catcaaatca
      241 gggccttgac cctgccagaa tatcctgcca gccatttcca tggaaataag cttccatttg
      301 tattgcatct aaatgacaaa tatattacga ttttattacg cttttggcgt aaatcaccgt
      361 atatgggagg tagactatga aaaccaaaat gttcacagct ttggcagcgg ccggtctttg
      421 tacggccatg gccttcaccg ctttcgcaga cggcgcgccc gcttcacaat tcggtttttc
      481 cggctggacc taccgtcagg aaacggattg cacaccccgg cagaccgtct gcccgaccgc
      541 cacgtcggaa cctgtcagca ccccaaagcc tgccgcgacc gttcagccgc cggtcattcc
      601 cagcgagccg acccgctgtc ccattcagga gcctgtgttg acgccggaat ctgcgcctac
      661 cgccgcaccg acgcaagcgc ccgtgatctc gccggttcaa acgcccgaag cgaccgccgc
      721 gcccacggaa acacccgctc ccacttctgt tcctacgaag aggcccacgc aacagccggt
      781 tcctaccgtg gcggaaacgc cagaacccac gagaaatcca gtgattgcaa cgccctctcc
      841 ttccacgggc aatgattata ctacgggttc catcactgcg caagaggaaa atgcatttct
      901 tttgctcaat caggaccgtg cggccaacgg tcgctctgca ctgacgctgg atccgtccct
      961 ttgcgcgctg gcccggctga agagtcagga catgaacgcg aaccattact ttgcccacac
     1021 ttcccccaca ctgggcagcg cggcggagat gcttcgaaac cacggctatg ccttcacctc
     1081 cgtcggggag aatatcgccc accacgccac cgtggcgaag gcgcaggccg cgtttatgtc
     1141 cagcaccgga caccgcacca acattcttgg tagtcagtgg acgaaggtcg gtatcggcgt
     1201 atgctccgac agccagggat atgtctacgt gacccagctg ttcgttcgtt aaaattcgac
     1261 aaatggaaaa gagacaggcg ttgtcctgtc acttttcggc ttaccatcgg gaaaagcgcc
     1321 ccgtccgccg gcccggccat ccgctttccg tttttcgacg cagataggga tccgcgccaa
     1381 taccgacggc ccgaaggacg cttcggcccg ccatgctgcc ccgaggccgg acgtaggtgc
     1441 tcaatgccgt ccggggcgtt tcctccaccg ggcggaaggg gtcttctgta ggacggacgt
     1501 gaagataccc gtcggaatag accggctccg ccgccttcat tcggggcaga gccggaagaa
     1561 gcacagcctc gctggtttgc agagcgggac gtttccaaag ggaacgctgg atcatgcaaa
     1621 ccctcctttc cttcatgcct tccatgctgg tttgggaagc tgcttcatcc tattcatttt
     1681 acggggaaac gtgtgcggcc agccattcct cccgggtaat agcatacacc cgggtaaagg
     1741 tattcaactc gtccgggtag gtctccacca ggcgcatccc gttctttttg gctacgccgc
     1801 aggaagcctt gttatcgtac ttcatatagg aatagacttc cgagagctgc aagtcctcaa
     1861 aggcatactg caggcctggg ctcgccgctt cggaagcata gccccggcgc tggtaacccc
     1921 gatgaatgtg atagccgatc tccggcagca tttttccgtg aacgcactgc atggccatgc
     1981 cgcagtcgcc gatcaggcgg ttttcttcct tcagaatgac cgcccacagg ccgaaaccgt
     2041 agttctggta gttttccaga tccccctcga gccactgccg caccttttct tcatcaaagg
     2101 gcgcgggata gtgcttcatg gtttccgcat ccgaaaggat cgcataccat gccgcgagat
     2161 cgtcgtttgt cagttcccgt aaaaccagcc gttccgtttc caaccgcata acgccacatc
     2221 ctttttaaga tgaatgaaaa caaaatcagg atcgcttcga agactgcctt cccgcgccag
     2281 taaaaatgaa attgaagaaa tgcaaatcta cattttattg taggggcttt tttgcatttg
     2341 tcaatggcgc atctgggttt ttgccgcatg gaagcctttt ccgcggcgca cactatggac
     2401 ggcaaactgc gcccttcggc agggctggta atggcaggga ggtctgaaaa atgcaatggt
     2461 gggtcgccgc agcgggcctt gcgctgctgt ttgttttctt tctcagtact cgaaaaaagc
     2521 cgtttttccc ccgtcaggaa tcgttttcag aagaaacgct gtccgccgga atgg
//
LOCUS       DAWAEA010000192         2564 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5839_length_2564_cov_1.838980, whole genome shotgun sequence.
ACCESSION   DAWAEA010000192 DAWAEA010000000
VERSION     DAWAEA010000192.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5839_length_2564_cov_1.838980"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    217..226
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2200..2313
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          495 a          752 c          758 g          435 t
ORIGIN      
        1 atcgacaatt cgctcaaaag cggcatcagc cagtccgacg cggccagcgg cgaggccatg
       61 cggcgcatcg tggacgcgtg gaagctgggg accgccaagg cggcgctgaa cgcccaaaac
      121 ccggtttcac aggtgctgag cactctgcgc cagcgggaac gcagcgacac catcaaggcc
      181 gtctgcatgg cccatcccgg cgaaaacgga cagaccnnnn nnnnnnaaaa tttgctgaat
      241 agcagtttga attgactcta ttgtaccgct tttcgggata aatggcaaga tgggagttgc
      301 gttccgcacg ggggtttgtg gtatactgtg acggtaggca ttgcgctcat gattcaggag
      361 cctttccaaa ggaggaaccg tcactttgaa atggttttct tttccccgtt ttcttcggcg
      421 ggggaacggg aaaaaagcgc cgtcccagcc cgaaaaggct gtggagacgg ctcatcagcc
      481 ggaggacgag aacgcccgga gcgacaaact gcccgccccg gaaaagggcc gcggccccat
      541 cacccataac ccggtttcac aggtgctgag cgctctgcgc cagcgggaac gaagcgacac
      601 catcaaggcc gtctgcatgg cccatcccgg cgaaaacgga cagaccgtca tcgccatcgg
      661 cttcatgggc accggcaagc ggatgtacga ctggatctcc aacctgcgct tttccacgga
      721 ggaaggcttc caccggggct tttatcagct gtgcaccttc tttgaggaaa gcgtggaaca
      781 gatcgttttc ccggcgacgg cggcccgtca ggggctggaa aagctgacgc tgggtgatat
      841 cctttcggat atgcgctccc tgcgcagccc ctaccggctc tggatggcgg gacacagtca
      901 gggcggcgcg gtgatgcaga tcttcaccaa ccggctcatt cagcactggg gcgtgcagcc
      961 gcagaatatg gtgggctacg gcttcgcttc gccgacggtg gccaccgggc ggcttctgta
     1021 cgacccggcc agctacccgc tgtaccacct gctcaattcc gatgatctgg tgccccggat
     1081 gggcggactg ctgcatttgg gcctgtgcat ggaataccct gcggacgacg cactgcggac
     1141 ggcggcctac gggctggatt ctctgggcat caacgaggaa gcgtaccttc gcctggcccc
     1201 ctaccgggac gccatgcagg acaccggcag cgtgctgacc atcctgtatg cctttctggc
     1261 ggcgcttcgg gagcatatgg aggagagtga accgggcaaa cggctggagc agcagcggct
     1321 gctgagctgg ctcggcaagg ccatgtcctt tgcgggagag aaagcctccg atgggttgga
     1381 ctgggcgctt cagcgcatcc gggaagggta tgaggacctg atgcaggctt ccctgcccac
     1441 acgaaaaatt catacgctga tcggcgaatt tcgggtgctg ttccgtgaga ttcctctgcg
     1501 gcgcatcctc agcgccattg ccgagctggg cgagccgccg caccacatca tgcgggaaaa
     1561 gcagccggga gcctatgcgt ggatcgtgga aaacgggctg gacaagctgc gtccctttat
     1621 ctggactacc caatccggcc aactgccccg gcgcagctat gcggaaggaa aggtcaaggc
     1681 tgtcgcagtc tggaacgaat atcccgtgcg ggtctcccgc cgtcatcgcc ggcccgcccg
     1741 gcgggcggag ccccgcggca ttcttcgccg cagctattcg gcccttcgtc ggagataagc
     1801 ctgtttcatc cccagaaaag atgctatact agtggacgaa atccatgaat acaaaagaag
     1861 gagggcgaag ccatgggagc gaatcagggc tttctgcgtc cgacaggcaa tctggcccgt
     1921 cgggaaaaga ccgtgtcttc gcctgctccg tctcagtggg agacgacacc ttatccctcc
     1981 aacgaccttc gggagggcct gaccaatagc agcgcccgtc catcgccggc cgcgctgcgt
     2041 tcccccctga ccccggccaa ggcgatgaag accgccacac ccgccccggt gcagccgatt
     2101 cccaaacaga caccgctgac cgaggaggat tggcagctgg aacggccggg tcgcctcgtc
     2161 cgccgggagc cggagaaagc gctgtccctt gtgacgagcn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaaaacgt cgccttccat ggctttccgc
     2341 ctactggaag atcgtcccgt ccccgatcag cccgaagaaa tgacagggga ggaagtatcc
     2401 gcgcctgtgt ccgtttcttc tctgccggaa acaccgcccg ccgaacctga acaggcgctg
     2461 agcgcttctc cgctggacga cgcgctgttt tccgacctgc tgcaaagcaa cccggatatc
     2521 atgggattgt tggaagaccc ggaggactgg ccggaggacg ggga
//
LOCUS       DAWAEA010000193         2548 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5879_length_2548_cov_2.534697, whole genome shotgun sequence.
ACCESSION   DAWAEA010000193 DAWAEA010000000
VERSION     DAWAEA010000193.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5879_length_2548_cov_2.534697"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2055..2064
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          469 a          790 c          780 g          499 t
ORIGIN      
        1 gctaactgct cgtcccacgc gtccagctga ttggcaaaat cgctgacgcc ctgcaatttg
       61 tcctgccgaa gcagcgcatt gcggctttcc agcacgctca ggtagttttt cagcgcccgg
      121 acatagccgg ggcggatctg gctgagctgc atatccacaa agcgccgccg ggccgcgggg
      181 ccgtcccgga cgatgcgcag gtcttccggc gaaaacatga ccaccgtggc gtggcccatc
      241 aggtcggcga tgcgctgggc gggcttgccg tacagaagca cccgcttctg gggcttttcc
      301 ataggggtca ggcgaatttc gatctcgtgc cgtccatcca gacggcgggt ctccccgtgc
      361 accaaagcgg ccgcttcccc ctgcgccacc atttcccggt cggcagtggt gcggtggctc
      421 cggcccagcg aaagaaggtg catggcctcc agcaggttgg tcttgccgct gccgttaggc
      481 ccgtaaagca cagtgatccc ctcgctgggt tccagttcca gatagggata attgcggaac
      541 tgcttcaggc gaagggtggt aaagtgcatg aaaaatgggc ctcgctttcc agtggggata
      601 aatttaccgg ctgatcttca ccgtgacggc gcgaccgccc agcggcttgc cgaacacgtc
      661 ccgacccttg accgcgtagc ggcggacgat cagcgcatcc ggcgcgtcca cggcaaggaa
      721 gccggtctgc agcatgggtt tggcctgacg ggtctgcaag atctttttca gggcgcggcg
      781 catggccttg tcgcccttgt cccaggccat gggagcccgg ttccacgggt cggccatgcc
      841 ctccatgccc agttcgtcgc cgtaatagac ggtaggcgcg cccggcaggg cgcacagcaa
      901 gcggaaggct tccagcacct tggcctgcgc ggcttcccgt tgttcgggag tcagccggat
      961 atgagcggcc tccgcccgat ccatctgggc aataccctcc tgatcgtagc ccgccatggc
     1021 gttgaggatg cgcaccctgt catggctgcc cagcacattc atcaaagaat aataaaacgg
     1081 cgcggggtag acctcctgct ggtgcaggat gatccggcga agctgctgcg cgtcgcattt
     1141 gccggtgaaa aagtcgatca cggcccggcg caggggatag ttcatcacgc tgtccagact
     1201 gtcgcccaga cagtaggagc gcagcgcgcc gtaggacacc ttgttggacg cgtcctccca
     1261 gacctcgccc agcagcaccg cctccgggtc ggcctgcttc acggcccggc gcatggcgga
     1321 caccagcggc atgggcagct cgtccaccac gtccagccgc cagccgcagg cgcctttttt
     1381 cacccagcgg ggcaaaatgc cgtcctccgg gtccagcaga tagcgctgat aatcggcgtt
     1441 ttccttattg acggcgggca aggtgtcgaa gccccaccac gtgcggtagc ggtcggggta
     1501 ctgctcgaag gtataccacg gataataggg cgattccgtg ctctggtacg cgcccagcga
     1561 atcatagcgg ccgtagcggt taaaatacag gctgtcggag ccggtgtggc tgaacacgcc
     1621 gtccagcatc acgcgcattc cgcgggcctt cgccgcgcgg atcagcccgt cgaaggcctt
     1681 ccggtcgccc agaatggggt cgatctcggc atagtcgccg gtatcatagc ggtgattggt
     1741 gcgggcccgg aagatggggt ttaagtacag caccgtcacg cccagctcct gcagctcatc
     1801 caacttttca gtaatgcccc gcagggtgcc tccgaagaag tccagcgccc ggttgtccct
     1861 ggtgacgggg tccggatcca gcagcggcgc ttcgtcccac tttttgtgga aaaccgcctc
     1921 ggggtgtgcg gctttgatct tgcggctccg tcccggatga ccggcctttt catcccggaa
     1981 gaaacggtcg ggaaaaattt gatacaccac ggcgtgacgc aaaaattccg gggtctgata
     2041 ggccgggtcg tacannnnnn nnnncgtaat aggcccccac gcctcccagc tgatcgtagg
     2101 acgtgccata gcgcgtgtcg ccgccgggcc ggtgaatgat gaaatcgtac cagaacagca
     2161 tgggcttttc aggcacgaga atcgtgcttt caaaaagatg ttcccgggtc tgctgcatgg
     2221 ggtagcgggc ctcgccgccg tcccaggtgc gcagagtgac gtgttccgct tcgtcgcaca
     2281 aaaagcggat ggtgacggaa gcgcctgcgg gaaccgggcc gatggggtcg cgatactccg
     2341 catcgtagga atcatgataa agcatggctt cccgttcctc cttcatggct ggctttgcat
     2401 ttacgcccta tcatatcatg aaaccggcag acggtgcaaa cccttcccgc ataaaattcg
     2461 gctggagata attcccgctg ccctgacgaa cggtttccag tagagccgtt ctttccattt
     2521 ttatgcgcaa atctggcaat gattgtcg
//
LOCUS       DAWAEA010000194         2537 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5906_length_2537_cov_1.992345, whole genome shotgun sequence.
ACCESSION   DAWAEA010000194 DAWAEA010000000
VERSION     DAWAEA010000194.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5906_length_2537_cov_1.992345"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    371..451
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          431 a          743 c          718 g          564 t
ORIGIN      
        1 acgcccgcct gaggccagac ggcataattg gcgttactgg tataggtcgt aaacggattc
       61 aaaatggaaa tggtattggc acggctaaag gtagtcacac tcttaccgcc gctttgtaca
      121 gtcgctttca gcgccattgt ctccgattgc ggatccgctt cttcgaccgc ttccgctgta
      181 agctccggca gcggcgttgc gtcaggctcg gcagtgggtt ccggggtcgg ttcaggcgtg
      241 gccgtaggct ccggcgtatc cgtgggagcg ggcgtatcgg tcggtgcttg cgtaggcgct
      301 tccgtttcta caggagcatt cgtctccacc ggcagcgcgt cgccgccgag cgccttgatg
      361 tccttcaggg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgcccgttt gaacggaagc atcgtcttcc
      481 gcagcgaagg acacggttgc ggaacaggtg ctgtccacgt attcggcgtc tgcatccgtc
      541 ctgtactggg caaaataagc gccggtatag tcgtcggtca gttccgcctc caccgtccag
      601 gtgcgaacgc cgtcagcttc ggacatctcg cagttttcat ccgtcagctg catctcggca
      661 acggatgcgt cgtactgatc caccacgcgc acagccgtta ctgtatcgga agtacggaag
      721 gtaaaggtca caatagacgg aatggtacca ttttcgggct ggcaggtgaa atcctgcatt
      781 tccggcaaga ccgccttggc cgccgcaata ctgaccgtag taccggtgcg gacgccttcg
      841 ctttccgttc cgtcattctt gacggcatag acggtgaagc cgcccacata tgcgtcttcc
      901 aaggtgcagg taggcatcca gatcagttcg ttgctgttct cgatggcctc gccttcctcc
      961 accaacgcct gactgtaatc tctggcatac agcgtattcc catcgtcatc ggccacgcgg
     1021 acgcctttaa tattcttgga ggtctgaatc gtcagccgga gggcggcagg cgcttccgta
     1081 tccgaggtgg aagccgtgaa gctcttgatg ggttcttctt ccggaacgat catgttcttc
     1141 acgttggaga atgccgttac cacagcgttt ttcacgggag agatcagttg tcccactttg
     1201 ccgccgctgg actcccagtt cggcagacaa atacccgcca gcagaccgcc gatcaccagc
     1261 aggacgatca gaatcggcag gagcacaccg ccacggcgct gttccacttc ttcttcctgc
     1321 tcgtcgtcgt tttcaaagtc gtagggtttc cgtgggaccg gcggctgctg ccgactgtcc
     1381 tcgccataaa cgggacggcc ctgcgcccga taggcatacg gctgttcgct tttttcctgc
     1441 gggacacggg tataattttc tccttcggct acaggacggc gcggcggaac cggccgttgg
     1501 gcgggggtgc gcatttcctg gccagccggg cggcgctgag ggacagtggc tccctgtgcg
     1561 gagcgcggtt gcccgttctg gggaacgttc tgcccagcag tagaaggccg ctgagccggc
     1621 cggcgctgcg gattcggtgc gccctgagga cgcgtgctct gacgagcgcg aggagccgcg
     1681 ccattctcgg attgtgagac ttctccgtgc ctttcgggag cggctccgcc gcgaggcatt
     1741 gccgtacgtg cccccgtgta cacaccgccc ggaacggccc tgcgggactg aacaggcatt
     1801 tcactgtcgc ccgcctggaa gggatcaaat tcaggctgaa cctccaccgt ctgttccgct
     1861 ttaggagctt ccgccgactg ttcttcttcc tgttggcggc gttccatgcg gctgcgacga
     1921 cggcgcggcg gttccgcgcc atcggaagag gctttttctt ctgcgctctt ttcggtcgtt
     1981 tcattggcag aaacggcaga agtgctattt tcggaagctg ctttttcctg cgcttccggc
     2041 tcctgcacgg aagtgcggcg gttgaccatc tgcacaggcc gcgcgatccg cgtaggggca
     2101 gcgtcgccca tcatacggaa atcggacata gccgcattgg acagcggtac gccgccctgc
     2161 tgaatctccg ggccgaaagg cgcaggatcg tcatccagga agctgccgct tccaccgctt
     2221 gggcggcgcc acgccgaata cgggtcagta ccatcggaag actgtgcgcc ttcgccattc
     2281 gcataggccg ctttattcca ttcggaattg gcttcctgag caggcttttg gtaaaagtcg
     2341 ttggaatcag gctggtcgcg atagaaatca ctcaatttct tgcactcctt atcatccttg
     2401 gttactcccc cggggaaccg gctgccttca caaaacggga acagctctcc cggcttcggt
     2461 cgtttcatag gattgacgca tctgtcaatg cgtataccac catttattat accaatattc
     2521 ccattgcaga acaagcc
//
LOCUS       DAWAEA010000195         2536 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5911_length_2536_cov_1.841193, whole genome shotgun sequence.
ACCESSION   DAWAEA010000195 DAWAEA010000000
VERSION     DAWAEA010000195.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5911_length_2536_cov_1.841193"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    707..716
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          620 a          629 c          758 g          519 t
ORIGIN      
        1 gaggtgaact gctccatcat gaagcggctg gcgctggcct gccgggatgc gtggatgaag
       61 gccatgtacg tttccaacgc cgtgtggctg tcctccgaat gcctgacgca gatcgtgttt
      121 tcccttctgt acattgcggg cgtatactgg ctgggcggca agatggtctc cttcggcgtg
      181 atccttgcca tggcttccta tgtgagccgc ttctggcagc ccatcaccaa tctggcgaac
      241 atctacaact cttttgtgaa taacatcgcc tatctggagc gcattttcga gaccatggac
      301 gagccggtgg aggtgaagga cgttcctgac gcggagcccc tgaaggcgct ggacggcgaa
      361 gtgaccttcg aaaacgtgga gttcggctac gagaagggcc agactgtgct ggaaaacctg
      421 aacctgcatg tgcgggccgg ggagcgcatc gcgctggtgg ggcccaccgg ggcgggcaag
      481 tccacggtga tcaacctgct gtgccggttc tacaacctgc ggggcggacg cattctgctc
      541 cactgcgccg acggaaccac gcaggacatt gcgggcgcga cgcttcattc cctgcgctcc
      601 cagctgggca tcatgctgca ggacagcttc ctgtttgcag gaaccatcat ggacaacatc
      661 cgctacggac ggttggacgc tacggatgag gaggtcatcg ccgcggnnnn nnnnnnacgc
      721 cacgccggtc aacgagcggg gcggcggtct cagtcagggc cagcggcagc tgatcgcctt
      781 tgcccggacg cttttgtccg acccgcgcat cctgattctg gacgaggcca cctcctccat
      841 cgacacccag acggagcgcc tgctgcagca gggcatcgaa acgctgctgc agggccgcac
      901 cagcttcatt gtggcccacc ggctgtccac catcaagagc tgcgaccgca tcctctacat
      961 cggcaacaag ggcatcctgg aagcgggcag ccacgacgag ctgatggcca agaaagggta
     1021 ttattacgag ctgtataccg cccagacgca ggcagcctaa aagagaaacg aaaaacgcgc
     1081 cgcacggaag gaaaacgtgc ggcgcttgcc cgtcgcagaa aattggcgga atatttggca
     1141 ttccgtattg actgtcccct tacaggccgg tttacaatga gggggaggtg agaaggatgc
     1201 ttatcaacga ggcggcgagg aaatgcggta tcaccaaaaa agcggtccag tattatgtgg
     1261 agcaggggct ggtcgatccg aaagtgctgg aaaatggata tagagatttt tcagaagagg
     1321 atacgaaagt actaaagcgg gtcgttctat atcggaaatt aggcttgagc atccgggaaa
     1381 tccggcgtgt gcttgaggat cgcggggaaa tcagcggcat tttttatcaa agagagctgg
     1441 aactggagca ggaaaaaacc aaacaggaat ggttgaagcg gctggcggag aagcaatcga
     1501 ggatcttgag ccggagattg atccgctcag ttccaatacg atcattatcc aaaaactggg
     1561 cgagctgttt ccggggtatt atgggaaaat catccgtctc catttttcca gatatctgac
     1621 gggaaaggtg gagacggagg aacagaaaaa agcctttcat cagatcatcg aattctttga
     1681 tcgggttccg gagatccgtt tgccggatga tctgcgggaa tatctggatg cctgtatgga
     1741 agaaggctcc ggagaagagg aaaccgagag gctccggcgc gttttgcagg aacaggagta
     1801 tgcccttcag aatctggacg agtttgtcag gaatcataaa aaggtattgg atgattacca
     1861 aaaataccgg caaacggagg aatatcagaa ttcgcctgcc tgccggctga tggaaacgat
     1921 gaaacagatc tgcgcggcca atggatatga cgatacgttt atccccgcca tgagaaagct
     1981 cagtccgctg tataacgagc attacgaaca actgctgaaa gcaaacgagc gatttacgaa
     2041 aagctatccg gaatatctgg catagcggtt gccgattcag acggggctgc tttcttccgg
     2101 tttaggaaag agcggaggaa acgcatgaaa gaggcttatt tcgtttatcg acccggaccg
     2161 taaaaaatac gctcaggagg agaagcaatg tactatcacg catcgccgtt cggcgggatc
     2221 acccaactga agccgcatgt ttccaatcat gggatcccgc tggtctactt ttcgaaaaaa
     2281 agagaaaatg tattggtgta cttgagcaac gccgttgaga agtattgtaa ggagaccggc
     2341 tttttgtatg aggggatcta ccagaagtgg ggtccgtatg gctttaacag ggacgggcgc
     2401 cagcggctgg aggaatacta ccccaacgcg ctgatcagta cctatcaggg cgtgtccggt
     2461 tatatctatc gtgctgagac catttcggag gccggttttg ccgttccaat cccggatgcg
     2521 gccgcaagca gcgtgc
//
LOCUS       DAWAEA010000196         2535 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5916_length_2535_cov_1.610484, whole genome shotgun sequence.
ACCESSION   DAWAEA010000196 DAWAEA010000000
VERSION     DAWAEA010000196.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5916_length_2535_cov_1.610484"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2154..2306
                     /estimated_length=153
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          457 a          755 c          707 g          463 t
ORIGIN      
        1 agccgggaga tcctgatccc cgtgctggaa aatcgcaaca acgccacgtg gatccagcag
       61 tgcaaaggca ttcacgagga agaaagctac acgctgctgg cccgcgacac ctatggccgg
      121 ggcgaaatga ccaccctctg cgtgccggaa tccttctccg acctgaaata tctgccggtg
      181 cccatgttgt cctatatgcg tcgggagttc cgggtgaacg gcgtgtatct ggaaggcgcg
      241 cccatgatca gcctgttcca ctacgataac gacagcttta tcctgtaccc ctatctggat
      301 cgggagaccc acgataccga tattttcctg catgtgcagg acgccaaggc cctgacggag
      361 gtggtctccg gcaagcgggt cgaaccgctg tacaccgaag cgggcgaggc tgtgttccgg
      421 atccatgccg tggtgggcaa gttccgcggc tatcatgtgg aacggtaaaa ggtctctgga
      481 tggaaaaacg gagaggctgt atgtccgctc aaacgccgca ggaatgccgt aggtacgctt
      541 tgcactgcat ctgcgcatag gcgttgcctg ccggagcttt aggagcaaag tgccacccct
      601 ttccgcgaaa cagccaaaac agaatctcat caaaaatcaa aaggctcggc cttccttatc
      661 gagggccgag ccttttggcg ttcctttcag ttttccagct cataaaccac atattcttcc
      721 cggaagcggt agcccagcgt ttccgccaga tgggcggagg ttttgttggc cgcgtcccag
      781 ctggggaaaa gcccccgttc cagacattcc agcatcagcc gggccccgca ggcccgcgcc
      841 accccttcgg gcagccgttc cgccatttcc cgcaggtctt tgggcacgaa gccctccgca
      901 ctgtgctcaa aggcgtatcg ggtgcgggga gaagccgcct tgccgaagac acgccgaatt
      961 tccgccgccc atgcgtcgtt ctggggtgtc aggaggacgt atgggcgtcc gtcaggccga
     1021 aagcgcagca gttcttccga gggctgaccg acgaagaaag caaagtcgcc cagcactgcg
     1081 gcggcggagc aaaggggcca ttcggctacg gcgtacacgc cgcccattat gccgctcagg
     1141 caggaaagta ccatcgtatc ttcaaaggag ccgaacagtt cggcggcttc cgcggggttt
     1201 tccagccggg taatcatggg cagccctccg ttctgaatgg gggaatcatt caatgggaat
     1261 ttcagcggcc tgtgcgatgc cgttcagata ggtcttgaag gaacgggaaa agcccgtcac
     1321 cacctgctgc tggaaggcgc tgtccggggc gctcagctct ttttccagcg cgtcccgcag
     1381 ttttttccgg gtggagtcgc tgcgcaggtg cttgaccaga ttccgtttgc gcagaagcag
     1441 gggcagattg gccttcatca tggccccggt aagcgcgtcc ataccgaaca gtgacacagc
     1501 cgtgcccagc agcgctcccg ccagaaagcc cagagggccc gccgcgatca gcgccacgcc
     1561 gctgccaccg cacagaaggc ccagcagaac gctgacaatg accgtcatca gcgcgccgat
     1621 caggttcatg cccgtcagcc ccttcgcgtc cagcggcagc ttttccgggc cggagctgac
     1681 tgccgtcagg ttcagggcca tttccttgcg gggtacctcg tagcggtcgc agatgggatc
     1741 caccagcgcc tgcagctgcg tccccacttc atccagccag ctgcgcaggg ccggggtcag
     1801 cgcatcccga gccatggggg agtccagcac ctccgtgacc cgctgcttga gctgggcgtc
     1861 catttcttcc attgtgccga tacggccctt ctgccagtcc gttaccacgg gcacggcggc
     1921 ttcctcgatc aacagattca ccagcgcctc cgttacaggc gggatcagct ggggcatggc
     1981 cttgcgagcc gtgtaggcga cccgttcctc ggtgatctcc ttttcgatgg ccttgcgaaa
     2041 cgcccgcagg ttggagtcga tccacccggc gtagctcaag cccttggcaa tggaaaattc
     2101 cggctccggg cagcagacca ccgccgcgtc ccggaaggac tcacggcaaa gctnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnngtcc tgcaccgcct gggtaaagct gcggccctgc
     2341 agcgcgttca gcggcttttc cgtcagctgg cgcgccaagt cgccgtccag cttgagagtc
     2401 agcttctgca cgccgtcgta gcagatgcgc agcacttttt tcaccgaggc ggacggatcg
     2461 tccaacaacc gggtatagta ttcttccttc aggcggcggg cttcgatttc gcaatagctg
     2521 taccagcttc ggctc
//
LOCUS       DAWAEA010000197         2534 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5919_length_2534_cov_1.943526, whole genome shotgun sequence.
ACCESSION   DAWAEA010000197 DAWAEA010000000
VERSION     DAWAEA010000197.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5919_length_2534_cov_1.943526"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          521 a          776 c          784 g          453 t
ORIGIN      
        1 gcctcatgtc cttcgaatgc tcccgcgccc tatgggcgcg gggtggggtg caggggcact
       61 gcccctgcct agcccctgct ttcgcggaag taggcgttgc ccagaccggc cgggggctgg
      121 tcttcccgct tcttgcgcag gctggtcagc accagaacaa tgatcgtgac cagatagggg
      181 agcgccttga agatctcctg catactccgg tccagaccgg gaacgtagag atagagaatg
      241 taaagcccgc cgaagaggac gctgccccag atggcgctca ggggccgcca gcgggcgaag
      301 atcaccagcg caatggccag ccagccgcgg tcgccgaaac cgttgtttac ccacgtgccg
      361 ccggtgtact ccatgacgaa gtaaagtccg ccaagcccgg ccagcatccc gccgatgcag
      421 gtggaaagat acttgtaaag aggaacgttg atgcccgccg catccgccgt gccgggattc
      481 tcgcctactg cccggagatt cagacccacc cgggtgcggt agatcatcca gctgaggatg
      541 atggtggcaa tgatggcgaa gtaggtcagg aagccgtagc tgaacagcaa ttcgcccacg
      601 acgggcagat tgctgagaaa gggaatcttc gcccggaaag cgctggccgt gaccttggtg
      661 gagatctgac ccacgccgcc cgccagcttg gatagggagc cgccgaagaa gttgccgaag
      721 cccatgccga aggtggtcag cgccaagccg gtcacgttct ggttcgcccg cagggaaatg
      781 gtcagcacac tgtagagcag tgcgcccagc ccggagcaga ccagcgcgca gaggagcgca
      841 atcagcatcc ccacgatggg agaggggttc gccgcgctgt tttcataaag gaacgcgccg
      901 atcagcccgg caacgccgcc catgtacatc atgccgggaa cgcccagatt caggttgccg
      961 gttttttccg tcagaatttc gccgttggcg ccgtagagaa tggcgatgcc ctgcgcaatg
     1021 gccttttgca gaaatacaat gatgatgctc acgccttcgc cgcctccttt tcccgcttgc
     1081 gcagctccac ctgatagtgg atgaagaact cacagcccaa aatgaagaac aggatgatgc
     1141 ctgtgatgat gtcgctggcg ttttcgttca gattgaactg ggtagcaatc tgtcctgcgc
     1201 ctttttccat aaagaccaag aagaaggcta caaagaccat ggcgaaggga ttcagcttgc
     1261 ccagccacgc cacgatgatg gccgtgaagc cgcgcccgcc tgccagcccg gtggaaatgg
     1321 tgtggcccac gccgctggcc agcagataac ccgccagacc gccgatgccg ccggaaatgg
     1381 ccatggtgcg gatgatgacc cgcttgacgt tgataccggc gtagtgggcg gtgttttcgc
     1441 tttcgcctac cacggcgatc tcatagccct gcttggagta gttcatgtac aggtacatca
     1501 gcaccatcag caccatcacg atgatgatgg gcagcaggta gccgttgccg ccgatggagg
     1561 gcagccagcc ggccttggtc tggttgttga ttaggcccac gtggttggaa cccttggggg
     1621 tctcccagta gacgatggcg aaggatacca gctgcatggc gatgtagttg agcatcaggg
     1681 taaagagggt ctcgttggtg ttccagcggg ccttgaaaaa cgcaggaatc atgccccaca
     1741 gactgcccgc cgcgattccg gcaatggggc agagggcgta cagcgcccag ctgggaaggt
     1801 cggcaccgta gatcatcacg gcggcggtgg ccatggcccc caccagcacc tgcccttcgg
     1861 cgccgatgtt ccagaagcgc atccggaagg cgggcagcac cgcaaagccg atgcacagaa
     1921 gaatggccgc gtcccgcagg gtgacccacg tgcgccgggc cgtgcctacc gcgccgctcc
     1981 agatcccggc gtatacgctc aggggattca tgcccgtcat gcccataatg acgaacgcgc
     2041 agaccaccag cgccagcaaa atggcaacca gacggatcag cgacgcacgg acgaaggtca
     2101 cgccctcccg cttgacgata tgaaacaggg gttctttggt ggatacctga ctcatgcttc
     2161 cgcctccttg tccgcttcgc gatagccgtg gttggtcatc agcaggccca cgtcctcctt
     2221 ggtggcggtg cgcgcgtcta caatgccgct gatcttgccg tcagccagca ccagaatgcg
     2281 gttgatgtcc agtccgcgga cgggataggc caccatcagc acctgcggag aagaagaaat
     2341 ctcacggcct accagcacct tctgcacgtt gccgccgctc atgcggccca cgggccattc
     2401 cacgctgggg gtgaccactt ccagctcctt catgatggtc tcggccagct cccggggctg
     2461 cttgcgctcg gagaagaagc cgtggccgtg gcggtaggag cgcagcatca tgttgtccgt
     2521 catgcccatg gagg
//
LOCUS       DAWAEA010000198         2516 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5958_length_2516_cov_1.909386, whole genome shotgun sequence.
ACCESSION   DAWAEA010000198 DAWAEA010000000
VERSION     DAWAEA010000198.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5958_length_2516_cov_1.909386"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          572 a          677 c          818 g          449 t
ORIGIN      
        1 ttttgcgaga attaaaaaga acccatatca atctcaagag gaacgcccgg tcatccggga
       61 aggaaaggac ggaagaatat ggcggcgaaa caacccaagg tggaaccggt caaaaagccc
      121 tatctgaagg gctctttctg cggaaaggac gcatggaaga ctgtgccgaa gcgctttgca
      181 ggcatgttga tgatctgctt cgtttacgcg attgcgtcga tgattatgaa ctttgacagt
      241 ctggcgatgc gcatcgtcgt gtcggtgatg atcgtggctg ccgcactgta ctacgtgttc
      301 gccaagggca tggaacaggg caccggcgat gtgggctacg gcgaaatgat gtaagctcgc
      361 cagcaggagg gcaaaaccat cgacaaactg gacagcgacc gctgctatca tccgctgaag
      421 ggcttttttg aggcgctgct gggcgcgctg ccctatgtgc tggtgtcgct ggtgttcgcc
      481 gtgatgacca agcccaccac ttattcactg ggcacgctgc cctcctggac gcaggaaatg
      541 cttttgcagg atgaatttgg cgacgcgctg cgctactatc aggagactca cgccttgacc
      601 gcactggaaa ttctgcgggt catcgtgcgg atcatgtgta tgcccatgat gagcatcgct
      661 acctatctgg gcaccgacgc ggctctgctg gccgagcgcc tgagccccct gttcctgctg
      721 ctgcccgccg tcgcctacgg cgtgggttat ctgcagggcc cggtgcagcg ggagcgcatc
      781 aataccggca tcaagatcgg cgtgaacaag aagcagcgca agcagcagcg ggaaaagaag
      841 gcccgcaaaa aggccagcgc caaaacgccg gaaaggctga tctgacccat gaggatcatt
      901 gcgggaagcg cccggggaac gccccttttg gcgcctaagg gcatggacac ccggcccacg
      961 caggacaagg tgaaggaatc cctgttcaat atgctgcagg gccagctgga agacggcgct
     1021 gcactggacc ttttcgcagg cagcggcgcg ctgggactgg aagccgtcag ccggggcgca
     1081 agccgggccg tgctggtgga tcagtcgcgg gaagcggccc agtgcatccg ccgcaacatt
     1141 gagaagctgc gctttcagga tcgggcggag ctgctcacct gcgattggaa acaggcggtt
     1201 tcaaagctgg cccgagaggg acggcggttc gatctggtgt ttctggaccc gccctatcgg
     1261 atggacgact tggggcctct gtgcgacgag ctggacgggg ctgggctgct gaacccggat
     1321 gccgtcgtgg tgtgggaacg ccgcaccggc acgaaaaacg agctatctcc ggcgtttgca
     1381 ctgatgaaac agcgcgccta tggggacacg gaaatgcttc tgtaccgtta tgaaggccgc
     1441 gcaaaggagg aaggcgtgtg aaaggattat gcgtctatcc gggaagcttt gacccgccca
     1501 cgatcggtca tctggacctg atcgagcggg gagcctgtat tttcccggag atgatcgtgg
     1561 cggtgcttca aaacccggcg aaaaagaacg cattcaccgt ggaacagcgg atggacatgc
     1621 ttcgccgcgc ctgcgctcat ctgcccaacg tacgcataga cgctttcggc gggctattgg
     1681 tggattatct gcggaagacc ggggcggaga ccgtcctgcg gggcgtgcgc acggtcaacg
     1741 attttgagac ggaatttcaa atggcgcaga tcaaccggca gttatttccg aaggtggaga
     1801 cgctgctgct gcccgccagc ccggaggaag gcttcatcag ttccagtgcc gtgcgggaga
     1861 tcgcccattt tggcggggat attacgccct ttgtgcccgc tgaaattttg gaggatatca
     1921 aggccggtct gaacgcagac tgaccatggg aggaaagaaa aatgcagtct gacgatattt
     1981 tgaatttcaa acagaacatg agcgaactgg aagaactgat cctgcaggcc aagaaggtgc
     2041 ctctgagcgc catgtgcatg gtggacaagg atcggatgag ccgcattctg gaagcgctga
     2101 cgaaggagat gcccacggtt ttcactcagt gccagggcgt ggtggacaac cagatgtcca
     2161 ttctgaagga ggccagcgac cgtgcggagc agaccaagca gaccgccacg gctcgcgcca
     2221 atcagcttct gaacgacgca caggcacagg cacagaagac ggtggcggcg gcaaaccagc
     2281 aggcgcagga cacggtgaac aaggccggtc agcaggccaa cgccatgctg caggaggcgc
     2341 aggagcgggc gcagaacatg gtgcaggacg cggagcgccg cgccgcccag ctggtcagtc
     2401 agcaggagat caccgcccgg gccaacatgg aggccgaaga gctgaagcag tccacgaagg
     2461 aagaaatcga gcggctctat cacgacgtat accagcatat tgacgaagtg ctggct
//
LOCUS       DAWAEA010000199         2512 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5973_length_2512_cov_2.132275, whole genome shotgun sequence.
ACCESSION   DAWAEA010000199 DAWAEA010000000
VERSION     DAWAEA010000199.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5973_length_2512_cov_2.132275"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1113..1184
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          463 a          805 c          702 g          470 t
ORIGIN      
        1 acaaagaacc gtcccagatc ccgggaagcc tcaccagtgt tcaccgagtc ggagaatacc
       61 tgcagacctg cttcttcgat gaccccgccg tccacctccg ggatgcggaa gttgattccc
      121 ttccagcagc cgttttcaag ccggaagtct tccccctcat aatcccgatg atcgaaggcg
      181 gtgcgcacat agggtcgcat ccgaagcggc tccgtaccgc cccatttttc catcagaagc
      241 cgcatggaca gcgtctgccc cgggtagacc cgcggcgaaa aaaccgggct gtagcgcccg
      301 tcctcaaatt ccgcccgagt atagaaggtc ttgaaaaaca gccgggtctc catgcccttt
      361 tgcaggcggt tgaagaccac ctgcagagag cgctttcccc gataactctg cgccgtgctg
      421 ttgctcagct gcagcacaaa gggattgctg acctgcatcc cgtgggtgga accaggcagt
      481 tcaaagtcga agtgcagccc ttcttccgcg cgaagctcct ccggcacaga ctgactcagc
      541 agctcatagc cccgccgggc gatccgtttc gccagcgacg gcaggtcaca ctggttgatc
      601 tccccgctga ccccggaaag aatggccgta tcatgaaagg gctcccgata gcagggtttg
      661 atggggtcca gcccgccgaa agcgcccaga atggagccta cgttgcccgc gttgcagtcc
      721 gtatcccagc ccgccaacgt ggcgatctcc acgccccggt tcaggtctcc gccgctgtac
      781 agaagcgcca tgacgcacac gcccgcgttg gggatcatgg ggcaaacgcc ggagtagcgg
      841 tcatagcccc aatccttttc cagcatggcc cggcagtcct gccagcgctc cgggtgagcg
      901 tggtaaaatt ccagcaccgc gttcgtgacc cgggcataat cgccgtcttt ggggatggtg
      961 cgcagggcac gcagcacgat ctcctccacg ctctggctgg tgaaggccgc agcgttggcc
     1021 gccgccatga agcgtccgcc ggacacgccg tcgtcaaaat gggccaccgc cgccagccgt
     1081 ccgcccagtt ccgcggcgcg ctcgtcctgt ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcgcca gccccagaaa
     1201 atccacgaaa atttgtccgc cgacgctttc gtcctcgccc ttcagccccc gcaggtcttc
     1261 ccttttcagg ggcaacggtg cgccagagcg gatcaggtgg taggaggtat ggctggtgga
     1321 tttgcccacg ccgccccacc agaagaagcc ctttccctcc cgcacgaaat ccacccacgc
     1381 atgggcaaag tccaatagct gcgggtcgtc cttttcttcc agcacctgaa agaaatacgc
     1441 cgggccgttg atgtcgtcat ccgcgccgaa cacccgatag ggccgcagat agccgtggat
     1501 gcgttcgcca tataccgccc ggatgcgctc gtaagtccag ttttccgcct ccacaggcgc
     1561 gccgagacgc acgccgatgc attttcccag cacggcggca tacacccgct cggcatagtt
     1621 ttccggaagc attttctcac ctcactaaaa aacgcgggaa gggagggacg acgcaggggc
     1681 gttgccccta caccccacaa gggcttcgcc cttgaccccc tattgaccgc gcatagcgcg
     1741 gtcaaagtgg aggtacagga ggcactgcct cctgttgcgg ggtttggggg cgcacagccc
     1801 ccaaagtccc ttccttcccg cgaagaatgt ggatcattcc atcacggtcc ccagatactc
     1861 gctgatcttg tcagcgccgg cggcgttcca ggcctccaca aaggcgttcc aatcgctgtc
     1921 ggtattcttg tcgccgcgga tcacgtccac cgcgtactcg ttgaacaggc tgatcatggc
     1981 atcccagtaa gcggagtatt cgctgggcag gttcacggcg ttgtccgcca ggaagtactc
     2041 gctggcgata tccagagagg tcaggcccgc ttcgcccatg ggagcgtcgg cttcgtaggg
     2101 gatgttttcc agaccgatca ggctgtcgta gaacacgccg taccagtcgg gagcgccttc
     2161 gatgggcacg atcttgttat ccacgatgtt gtagtggaag ccttccacac cgtagagatc
     2221 cagcacgcgg ccctcggggc tggccataaa atcgaagatg gcgaaagcgg cttccttcac
     2281 ttcggagtca gcgctgataa cgcggccgcg ttcttccttg gacacgtcca cggcggcgca
     2341 gtactggccc agttcagact tggcgggggg cagcacggtc agggccgcgt ccgcgccgtt
     2401 ggtggcctgc atggagttgt cgtacacgtt gatggtcgcg ccggtcttac cgatcagcat
     2461 ggcggccttg ccggagtaga acttttcttc agccacgtcc catgtgttgg tc
//
LOCUS       DAWAEA010000200         2502 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_5996_length_2502_cov_2.165509, whole genome shotgun sequence.
ACCESSION   DAWAEA010000200 DAWAEA010000000
VERSION     DAWAEA010000200.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5996_length_2502_cov_2.165509"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          555 a          736 c          725 g          486 t
ORIGIN      
        1 ccgtccgcga ggttccgtgg gacgaagcca gccgcttcgg catcatgaac accaatgagg
       61 acaacagcat ttccgaattt gaggaaaagc ccgcccatcc caagagcaac aaggccagca
      121 tgggcgtgta catctttacg tgggagaagc tgcgccagta tctggtggcg gacgcggaag
      181 accccaacag ctccaacgac ttcggcaaga acatcatccc caagatgctg gacgaccagc
      241 agcgcatgtt cgcctatgat ttcgagggct actggaaaga cgtgggcacc atcgaaagcc
      301 tttgggaggc caacatggat ctgctgaccc tgcccatgcc catcgacctg tacgacgcca
      361 agtggcgcat ctacggccgc aaccccggcc tggctcccca tttcatcgcc gagggcgcca
      421 gcgtgcgcaa cagcctgatc accgagggct gcgaagtcta cggccatgtt gatcacagcg
      481 tgatcttctc cggcgtgacc atccgggagg gcgccacggt gaaggacagc gtggtcatgc
      541 ccggcgcggt cattgagcgc ggcgcgcagg tgcagcgggc catcgtgtcc gaggacgcgg
      601 tcatcggcgc gggcgcgacc gtaggcgagg ccacgggcaa tatcgccgtg gtcggtacgg
      661 gcgtgcatct ggctgccggc ggggaagtca aggccggaga gcagtatgcc ggttcctgaa
      721 accctttcgt tccaatgaat gatgaagtga ggaatccgtt atgaaagacg taatgggcat
      781 tatctatacc ggtgaaaagg acagctttct gcgcgaactg acgctgcagc gcgccattgc
      841 cgccgttccc gtggcgggcc gctaccgcgt gatcgacttt ttcgtcagcg ccatggtcaa
      901 cagcggcgtg cgcaacgtgg gcgtgatcat gcagaaaaac taccacagcc tgatggatca
      961 tctgggcagt ggcaaggaat gggatctgca cggcaaaaac gacggccttt acattctgcc
     1021 tcccttcctg acccgtgaaa atgtgggcgt ttacaatggt tcgctggatg cgctccattc
     1081 caacatgaac tacctgcgcc gcagccgtca ggactatgtg cttttctgca acagtctgat
     1141 ggtcttcaac cccaacttca acgaaatgtt ccaccagctg gacgacagct gcgacgcggt
     1201 gctgatgtac agcaaggacc cctctctgcg gcggcctgaa tacggcacct atctggacgt
     1261 ggacgaaaac ggcatggtga ccgacctgga aatggatccc acccatcccc ggtatgaaaa
     1321 cacctgcatg gacaccctgt tcatctccaa aaagctgctg gtagaactga tggaccgggc
     1381 cttcgcccac ggctatcacg gcctgatgcg ggacgtgctg cagcgcatga tccgcgacgc
     1441 gggtctaaaa gtgaagggct acgaatatca tgatctgtgc taccgcatgg acagcatcca
     1501 aagctacttc cagttcaatc tggacatgct caataccgag ctgcggcaca acttcttccg
     1561 ggaggatcgg cccatctaca ccaaggtgcg cgatgaaatg cccgcccgct acatggacga
     1621 agcggtcgcc gtcaactcca tggtggcgga cggctgcatc atcaacggtc gggtggagca
     1681 cagcgtcatc ttccgcggcg taaagatcgc caagggagcc acagtcaaga actgcgtcat
     1741 catgcaggac gcgcgcattg acgagggcgt gtatctggaa aactgtattc tggataagca
     1801 ggctcatatc cagaaggacg gccgtctgat cggcccaaag agctacccca tcgtgctgtc
     1861 caaaaacatg gtgatctgac ctggcgtttc cggcgcgtcc gttttcccga cggacgcgca
     1921 atttcaacaa caaggagtgg atattatgaa gatcctgttc acctccagcg aatgcgttcc
     1981 ctttatcaag accggcggtc tggcggacgt ggtgggcgcg ctggcccctg tgctggccaa
     2041 aaaaggccac gatgtgcgcg tggttctgcc cctgtatacc gccatcgact ccaagtggca
     2101 gcagcagatg gaattccagc ttcactttga ggtgcagctg ggctggcggc ggcagtactg
     2161 cggcgtgaac atgctcaaaa aggacggcgt gacctattat tttctggata acaagttcta
     2221 cttcggccgt ccctatgtct acggactggg cggagacgaa tacgagcgct acagcttctt
     2281 ctgccgcgcc gtgctcaacg ccatgccgct tctggacttc aagcccgaca tcatccatgc
     2341 ccacgactgg cagagcggca tggtgcccgc cctgctgaag atccagtacg cgcatctgcc
     2401 cttctattcc aagatcagga ccgtatttac catccacaac ctgcagtatc agggcatctt
     2461 cggcattaag gaagtgcagg acgtgctggg tctgggcgac gg
//
LOCUS       DAWAEA010000201         2498 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6008_length_2498_cov_1.783054, whole genome shotgun sequence.
ACCESSION   DAWAEA010000201 DAWAEA010000000
VERSION     DAWAEA010000201.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6008_length_2498_cov_1.783054"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1773..1782
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          399 a          822 c          728 g          539 t
ORIGIN      
        1 ggataagcct gccatccaca cgctgggtaa tcttaaagcc tacctgatcg ttgacggcgt
       61 acagctcgtt caggcgctgc agggaacggc cctcccgatc tgccagccag taatagctga
      121 agtctccgta caggatggcc ttgctgcccg ccgtgggcag gggcatgtag ttggacacca
      181 gcaccttctg gttgagcagc gtgtcgggct gccccgccag cagccccagc tgccagatga
      241 aattgccctg accgtccttc agcttgcgca gcagcttcag cgtggcgtcg ttcatgatcc
      301 acagcccttt gcggcggtat ccgatcttga gcgaatgctg caggtcgatc agctcgtccg
      361 ccgtaatggc cacggaagac gcggcggtca cgcccagctc cgcacccagg gtatcgtgca
      421 gcagaccggt gggcttatgc gtaccattgc ccgcaagaat ggcttcttcc tcggcggcgc
      481 ccacgcgacg ggtgaactcc cctgcgatat aagccgccat gtcgaaggcg ctgtcgtgga
      541 gcagctcctc ggagatgcgg atcatgcagc ccaccttgtg ggcgcccagg gtgatctgac
      601 caaaggcgtc gtcgctctcg ggaatggcgg cttcctcctc gatccaggaa gccacgccct
      661 tgctggtcac cagcgggatc ttgcgatcgc cggaggatgt ggtgatctta tgcaccagcc
      721 cgcgcatgat gttttcctct tccagcccct gaatcagctg attctcgaac tcgtccggca
      781 cggtgtagcc gccctcggac agcgtgccca cctgcagcgc gtcccgcacc tccgggctgc
      841 ggcggtccag attgcgcatg tgcgcccaga aggcgttttt gtacgcgtcg gacgcgacgc
      901 cctggcgggt ttccggcgcg cgcaggggac gggaagtcag ggcggggcgc acgggcgcgt
      961 tcagctctcg ctcgatctcc gcgacccgct cctcacgctc gatggcgtga cccagatcca
     1021 caacctcctg ctccatacgt tcatacgtcg cagtatcctc gccggacatc aggccgttct
     1081 cgccgctgcg ctcatccaga aacgccttcg ccttgtccca cacgtcgctg cgcttctggc
     1141 gcagatcaag aattttgctc atggtgtttt tcctccttct gatcattgga ttgttcattg
     1201 gaattggagt gctcatatcg ccggctggcg ggcatgagca aacccagccg cctttgcagc
     1261 tgggtcacgg gaataccgga gggttccggc ggggcaacat cggcggcgtt ttccacggct
     1321 tcgcccatgc cgccagatac tgccgtctgt tcgtcgtcag gcaacgccat cgaaggatga
     1381 gcttcgtccg cagtctcctg cttctcagcg cgggtcaatc tgggcttatg gcgatcctgc
     1441 catgcctgta ccttcgcctt ggcgaagtcc agatccgccc tgcgctctcc ggcgttctcc
     1501 ggcgcgccgg gcttgccgtc ctcggagatg acgtcaatga atccgtattg cagcgcggac
     1561 tgcgcgtcca gccacgatgt ggcgctcatc atggcggcca cctcctggcg gctctgctgg
     1621 caccggcgct ggtacacgtt caggatgctc tccttgcacg cctgcagcag tcggatagag
     1681 tccatgagct cccgctcgtt gccccaggcg acgacgctgg ggtcgtggat catccagaga
     1741 ctgcccgggg tcatctccag cctgtctgcc gcnnnnnnnn nntctctttt ccgtacagcg
     1801 cttcgtgaag ggactccggg gtgatctcgt caccgaacca gacttcatca tcgatatagc
     1861 cgttcaagct gagttctctc aattcgcatc ctcctccgtt tcttccgccc agacgatacc
     1921 ggccagcaca aaaaggacgc agtatacgca gaccgaattg ccgtaggcct tgtattccgc
     1981 gcagtccgtg cggggatccc tgagccattt gacgattcgg ttccggctgg gcgcctttgc
     2041 cgtcttgccc gtggcacgat accattcctc gaacacccct gcccagtaac gaatttcctc
     2101 gtctgttggc ctctctgtac ccagctgttc cgtccagccg tccggattcc cctgcaatcg
     2161 gcagcattcg cgtggtgtga gcctgcgcac gatgtagctc gtttcgggcg cgatcactgc
     2221 gccgggaccc tgcgccgtga gggtaggtac cttttccctt ccgatgttca tgctgtactg
     2281 cgcgttctga ccggcggaaa aacaggcgcg atccatggcg tatgccgcgc cttccggctc
     2341 ggagggtgtt tcatccatgc ccaccaaggg cggatccttg tagtcccgcg ccatgagcgg
     2401 cggggatttc tcctgatcca cctgcgtaaa gctgccggtg gtcatggcat agacggcgtg
     2461 gcggtctgac gtatccagcg tgaacgccac ctcgcggt
//
LOCUS       DAWAEA010000202         2491 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6022_length_2491_cov_1.898604, whole genome shotgun sequence.
ACCESSION   DAWAEA010000202 DAWAEA010000000
VERSION     DAWAEA010000202.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6022_length_2491_cov_1.898604"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          438 a          721 c          760 g          572 t
ORIGIN      
        1 ccttgggaca gtcgggatgc agataaatgg tcagctcggc ggtgtgccga tagccgcttt
       61 ggggacggat gggctccgcg ttggcaaagc ccagaggacg gcccgtttca tcctccgcga
      121 tcagaaaagg atattgtttc gaaagctccc gaatgctttc cgcataggcc gccacgggct
      181 ttgcgcggtc gttgtagctg gcctcggtgt tgtcgatgta gtagcggtac acttcctgaa
      241 gaaaggccgc gtcgctttcc cgggcggcgc gcagatggat attacccatc attttcccat
      301 gcctccgcac agcttgagaa tggccgcctg ttcctccggg ctgttgccca ccaatagggt
      361 tttgaaattg gtgtacgtca ccagacccgc cggggagttg gaggactttt tcacatattt
      421 ccgcagggtg tagtccaccg gcagggccgg gtgattgcgc cccttgctga agtaggccgc
      481 cagcttggcc gcgtacagca gcgtcgcgtc gccgggctcc tgctccgtat ggacgatcac
      541 atgagagccg ggaatgccct gtgcgtgaag ccaggtctcg ttgccccggg cgtgaagggt
      601 cagccggtcg ttctggatgg agtttttgcc cacctcgatg accgttccgt cgggagacgt
      661 gaagcggtag ggcttgccct cctgcagctt cttctgcttg cggggattgc cctccggccg
      721 cagaaagccg ttctgcgcca gcaggtagcg aatctcgccc atgtccgccg tggtctggca
      781 gttttccaga tcttccagcg cgctttccag cagcagcagg tctttgtcga tctgcaccat
      841 ctgctccgcg gcatactgtt ctgccacctt ggccttgcgg tattttttat agtagttctg
      901 ggcgttctga ttgggcgttt tgcgggcgtc cagcggaatt tcgacctccg gcatcccttc
      961 ctcgtagtag ttctgtacgc ggatgctttt ctggcctttt tccatcaggt aaagattggc
     1021 ggtcagcagg ttcttttcgg tctggtcgct ctgccgcagg gtctccagca tgatgctttt
     1081 tttcttttcc acccgctcca gcgcgctttt gatgtgccgt tgcagaccgg cgcttttctg
     1141 ctgcatccgc atccgcaggt cgcgccccag atagaaatcg tccatggcgc ggctcaggct
     1201 ttccatgggc ttctgatttt cttccgggaa gctgcggtag gggaagggga agaaatcctg
     1261 cgccagcccg gtctcgtcat agagtgtgac aggggagaaa cggtctgcca gacccgcaaa
     1321 gaagtccgcc agtttgccac acacgagagg aagattcagc tcgtccagct gggaggacgg
     1381 ctccacaccg atctgggcgc agacttcctt ggagcacagc cccgccatgc cgctgactga
     1441 ctccatcagc gccttgctca gggacatttt caggccggaa aggcggtcaa gcagcgtttc
     1501 ggcggagatt tcctccgggg tcagcttttc ctgccggggg ggaagatggt attcgtctcc
     1561 cggcagcagg gtgcgtaccc ggctcatttc gctgttgacg tggcggatgg cgtccacaat
     1621 cacgccgtcc tgccccacca gcgtcaggtc gctgtagcgc cccattactt ccagatagag
     1681 tgttttttct acgtggtcgc tcatttcatc cagcgcgtcg aagcgaacgg ccaaaattcg
     1741 gtcgccgaat aactgctcca cccggaggac ccgcgccccc agcaggtgct tgcgcatcag
     1801 catacaaaac atgggcggct cggcggggtt ttcataattc tgtcccgtca gctgggcacg
     1861 agcctgattg gcgttggcgc tcaaaagcag ccggtgattg cccccgccgg agcggatcag
     1921 cagcgtcagc gcgtcccgtt cgggctgatt gaccttatcc acccggccgc ccgccagctt
     1981 ttcatttaat tccctgacca tgaaggacag ggtaaatcca tccatcggca tgatgaaacc
     2041 tcctgtttgc cgcgccgccg gaggcttccg gcgtacgctt ccgtcatgat agcatatccg
     2101 gcgcaaaagt acaattcgtc atgttcgctc tctttggttc atacattgaa agtgcaatcg
     2161 acgcagagga gaggattggt ttatgaagat caaacgggcg gacattgagc ggatattgat
     2221 tctgtgcgcg gcggcgctgg tggtggttct ggcgctgcgg agcggcggac agaccacgtc
     2281 gcaggtgctg gtggagcaga ccagcgcgtc cacgaagggg gacacggttt ccgccgtggt
     2341 gtattacgag gacggggacg gctatctggt gcccgtgacc tgtcaggtgg aaaagacgga
     2401 cggcattgcc aaggccacgc tgaacctgat ggtcaagagc agcgaaaacg acatgcaggc
     2461 ggcacgtctt ggtctgcgga cggtgatccc c
//
LOCUS       DAWAEA010000203         2470 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6074_length_2470_cov_1.701035, whole genome shotgun sequence.
ACCESSION   DAWAEA010000203 DAWAEA010000000
VERSION     DAWAEA010000203.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6074_length_2470_cov_1.701035"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1909..2130
                     /estimated_length=222
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          463 a          655 c          721 g          409 t
ORIGIN      
        1 gatcagttcc ttgacgctgc ggaagggagc gaagcccatg accagcggtg acaccacggg
       61 acgcaaggtt tttctgcaca gtgcgaaaag acccgatgaa acgcaggaaa agcgcctgaa
      121 cgctttcctg tgcaagcggt acggggaagg cgcggaactg gtctgggtgg aggatcacac
      181 gctggaaaac ggcttccgtc tggaatccgg ggacgatgtg tacgactgga gcccgcaggg
      241 ccggttggaa cagttggccg accgcttgaa agcggtggac gtatccgaac agaatgtgat
      301 cccgcttctg cgggacacgc tggaaagctg gacgcctaag gcactggtgc acactaccgg
      361 caaggtattg acggtggagg acggcattgc cacggtttcc gggatgccgg acgctttcta
      421 tggagaaatt ctcctgtttg aaggcggcat tcgcggcatg gtgcagaatc tggatcggga
      481 tgagatcggc tgcatcctgt ttgatgacga cgcagcaatc cagcagggca gcaaggtgtt
      541 ccgcacgggc cgggtggcgg gcattcccgc cggcgacaat tttctgggcc gggtggtgga
      601 cgcgctggga agccccatcg acgggctggg ccccatcgag gccgacggct accgtcccat
      661 tgaagcgcct gcgccggaca tcatttcccg tcagcccgtg aatcagccca tggagacggg
      721 gcttttgacc atcgactcca tgttccccat tggccggggc cagcgtgaac ttatcattgg
      781 cgaccgccag accggtaaga ccgctattgc gctggatacc attttgaacc agaaggggaa
      841 gaacgtggtc tgcgtttacg tggctatcgg ccagaagacc agctctgttt cccagctggt
      901 ggaaacgctg cgcaagcatg atgcgctgga ttacaccatc gtggtcagcg ctacggccag
      961 cgactccgcg cccctgcagt atatcgcccc ctacgcgggt tgcgcgctgg gcgagtactt
     1021 catgcagaag ggccgggacg tgctgatcgt ctatgacgac ctgagcaagc acgcagtggc
     1081 ctatcgggcg ttgagtctgc tgctgggccg ctctcctggc cgtgaagcct atcccggcga
     1141 cgtgttctac ctgcattccc gcctgctgga gcgggcggcg cacctgtcgg acgaacgggg
     1201 cggcggcagc atgacggccc tgccgatcgt ggaaacccag gcgggcgacg tgtcggccta
     1261 tattcccacc aatattatct ccattaccga cggccagatc tttctggaaa gcgagctgtt
     1321 tttcgccggt cagcggcctg ccgtgaatat cggtctgtcc gtctcccgcg tgggcggcga
     1381 cgcacagacg caggccatga agaaagccgt cggcgccatc cgtctggaac tggcccagta
     1441 tcgggagttg gaggtcttta cccagttctc cagcgatctg gacgagggca ctcagcggga
     1501 actgcagaac ggtcaggcgt tgatgcggct gctgcgccag cggcagtacc atccctaccg
     1561 gcagcatgag caggtcatcc tgctgatggc cgcgcaggga catctgttcc agcacgtgcc
     1621 gctgaatcgg atggacgaga tcgtcgcgga actgctccgt catgtggagc aggaggaatc
     1681 cgtcctgtgc gcccggatcg aggaaacggg caaggcaagc ccggacgaac tgcagcgtct
     1741 gcaaaagagc gccgcggcct atctgagcca gttcggcgcg cccgcagcaa aggagtgaga
     1801 aaccgtgcca agcgcccgtg agatccgcgt ccggatgaac agcatccagc agacccggaa
     1861 gatcaccaac gcgatgtacc tgatctcctc ggccaagctg cgcaaggcnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gccggtatct gtatccggac gagggcgagc
     2161 ttccgccgaa cggaacctat ggctttctgg tcatcaccgc tgacaagggg ctggccggag
     2221 cctacaacca gaatgtgctg aaggaagcac accgcctgat gcaggagcat ccgcagacca
     2281 agctctttgt ggtgggggag tacggacggc agtatttcca gcgccatcag gtgcccatcg
     2341 agcgcagctt tctctatacc gcccagaacc ccactatgca gcgcgcccgg gagatcactg
     2401 cggaactgct ggatcggttc gacaagggcg aattgcagaa aatctttgtc atctatacgg
     2461 acatgaaaaa
//
LOCUS       DAWAEA010000204         2459 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6100_length_2459_cov_1.779118, whole genome shotgun sequence.
ACCESSION   DAWAEA010000204 DAWAEA010000000
VERSION     DAWAEA010000204.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6100_length_2459_cov_1.779118"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          469 a          822 c          653 g          515 t
ORIGIN      
        1 ccaatagaga atatcccgta caatgagagt gtacctcgga tcatgttcaa caagcgggaa
       61 ccgattatat ccgcaggata aactgcacac tacactattg tgggaattgc agtaattggg
      121 aaaaacaatg cagtcgaaag aactatctct gctggcatcc agcaactgtt ccatattcca
      181 aaactcataa atacgactga tacggtctga aagtccttta tcatggtaca atatcgtctt
      241 tatcgcttcg atagcaatcc ctttcgggtc acaaactgcg attatcataa aaatccacct
      301 tatcttacaa atattatccg taagataagg tggattggtt tcataaattt gtggagtttt
      361 tttgctgcct gctgagcatc agctttccgg ccgcagcatt ggcgagcgtt gcggcaatga
      421 gaagccccag cgtgttccat ttggcgagga catccttggc acaggggttc agggaaaagc
      481 cggtttctat ggctttgccc actgcgggca gcaagccgcc ccagtaggaa aagtcgctcc
      541 accgggcggg ggtgatccag ggcgggagca accgtagcag ggcgggcagg acgagcgcaa
      601 gcagaaaagc gccgcccaaa acaaaccagc acgtttcctt tgggcacccc ctgcgcaccc
      661 ggccgcccag ggtgcgggcg gcggccagca gcaccagccc cgccgggcag agggcatagg
      721 ccgtcagggc ggccatccag gggaaaggga aatagagggt cacctgctgt gcgatccccg
      781 ccgcacgcaa aagctgctgc accgtctcca ccggcggcgc agcgtagccg ccccaaaact
      841 cgatgttgga aaagccatgg tcaaggcttt ccccgcacag ggccaggctg gcgctgctgc
      901 gaaagagccc gcacacgcgg tagatgtctc cttctatctc caattcctgc cccagcacgt
      961 cctcgctgcc ccagagctgc cacgcggcag cggttgagac cgcgcatttg ccattctcct
     1021 gcgcagcggg gaaccgcccc tccagaagca cggcaggata caccgcgtca tactcgccgc
     1081 aatatagaat ggcagtgatc ccgcaggttt cccagcggct ttccgccgat ccctctgcct
     1141 ggctccaaaa agcgggaaca agcgcgctcg ccccatcctc ctgccgcaaa gcagaccgca
     1201 gcgcttccag ctgccgcccc gtgaccggca cgtcggagcg caccgaaacc agcggatagg
     1261 ctgcccacag cctccccgca aaccaaacag cgatccccca ggaaaccgcg catgccaaaa
     1321 gcagcaccgc ctttcgaatc ttcatcccgc tgtacgcaca cgtgcgccct cctgcaagct
     1381 cttcgttgtg gcggccacca ccggcgcacc ggtcgtataa ttccgcaggg ccgccagctc
     1441 gttgttgcgc tccacgacct ccacataaac cttgcgcacg atgttttgca gccccaaaat
     1501 agtgttgcgc tcctccaccg aatagacgaa acccccgccg ttatcactgt gaagggcgct
     1561 gaccggcagg cagcagtcat attccgtctc ggaaagccgg attgtgagtt ccgcttttcc
     1621 cacctgccaa tccgacccgg tcagcgcagc ggaaagcacc acgcctccat cctgctgaga
     1681 aacggacgaa ttgattttca cgctttcgga ctttgctccc tgcatgaccg tcaccgtaac
     1741 gccggcggta tcctgcaccg cgtcctcggc ctccagcgca aatcggatct gataccctct
     1801 ttcggggtcg gaaatgctcc cgcccacctg gccgctggtc tgcccctgca taagctgcag
     1861 agattccacg gtacccgtct gcggcgcaca gaagacgccc tcctgctcgt ccaatttttt
     1921 cagcgtctcc acgctacttt ccatttcccg gatctgttcc gccagaaccg tggcactggc
     1981 cgcattggcg gcggctgttt cactggcggc ttccacgctt ttctcgtagg cgtgccgggc
     2041 gtcgtttcgg gcctgctccg cctgttcggc ggcctgcgca gccgtgcgag ccgcttcttc
     2101 ccgttggctt tggacgcttt ccagcgctgc gtccgcctcg ttgagccggg cttccgcttc
     2161 ggccagtgcc tcccggtttt gcgtttcctc tgccgtaagc tgcgtcagct ccgcttcgat
     2221 ccggctgatg atttcctcgt ccggcgattc ttcctgctgc agcaactccc gctgtgcctg
     2281 cttctctgcg atctcgcggg catattgggc ggccttttcc tgccgttcgt tcagttcgcg
     2341 ctgcagggca tcccgctcgc tgccggcgtt cgcttctttc tccgcccagg ctgccgcgct
     2401 gttctccgct gcctggtagg cggcctgcag ggcctgctgg gctttttcca gatcatagc
//
LOCUS       DAWAEA010000205         2442 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6159_length_2442_cov_1.518643, whole genome shotgun sequence.
ACCESSION   DAWAEA010000205 DAWAEA010000000
VERSION     DAWAEA010000205.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6159_length_2442_cov_1.518643"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    269..402
                     /estimated_length=134
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    994..1126
                     /estimated_length=133
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1928..1937
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          449 a          604 c          648 g          464 t
ORIGIN      
        1 cgaggccgtc tggtcagccg tactcccgtt tttcatgggc agatagccga tttggccccc
       61 gaagcccgtg ccgccattca tcagctgccg ccggtgcaca taggcccctc ccacgccggt
      121 gccaagggcg atgaagaaat agtcctcgaa ggtgtccgtc agcgcatagg ccgcggcggc
      181 cacgtcgttc agcaccgcag ccgggacgga gaaggtctcc tccagccact tcttcaaagg
      241 aacgcccgtg tagccgggga tattttcann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcctatat ccatggccaa
      421 aactttcatg tttttcctcc gtatgcgggg aaaagggagg aaagcttccc ccgctttcgc
      481 cgcgtcatgg ttttagtcat accacattgg gatggttttt gtcaatgact cggcggtacg
      541 gtgtccgggc ggcgtttcta ctcaaacgca cacgcccggc tcgatggaga aaacgtgcca
      601 tagaacgctt gtttttcggc cctgaaaggg aatagaggat aagcggaaag cccctggtaa
      661 gcggctatgt tcgacaaact ttgccgtgat actgtgggct taccatccgg gtgctccccc
      721 atcttgcatc aaatggtgtg gcttgggatg tttcaaggtg gctggaatgg ttttgcagcg
      781 cagagaaaca agcttagttg ccgacctaca ttgggcgtga actataccca attagcacca
      841 gatgaaaagt cgtgtggtag ttggaggatt tctgtaacac caaaggccag cgcgggttgc
      901 aaatccacgc tggcctttgg ttcgagccat gaggcccgcc gccgccgaat ggcggtcttt
      961 tttattcaac tgacaaggaa accacaccga accnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtat ttcgcagctc
     1141 tccctatgcc cctgcgcgcc gtaggcgcgc agtccgaacg gggggctcag tccccgattt
     1201 tgccgagaac gatgaaacgg gcgccatcga aggtgtagac acaggtggaa agcataagaa
     1261 gccggtcttc gaaggtcact tctatgcccg tgtcgtaagg cgtggaggtg atagcccggt
     1321 gaatgaactt gttgaagtcc tcctcggtgt cgaaattggt gggataggta aacgtgtagc
     1381 cattcactgt gtaggcggcg aaaacggcca cgtcgtaatc gccgtcaggg gtcatcagac
     1441 ggatggtctg gtgagcctcc cagtactcgc gactttcgta ctggtgaaga ccgccgaaca
     1501 tggagccgcc acgcatgtgg tggccgtgaa gaatgttcac cgtgtcgctg aaatcgccgc
     1561 ggttggcttc gtccatcatg atggaaccga agcggttctg cgtaccgtcg ggaaaatgct
     1621 tcagatagta ctcgttgtcc tcgccctgaa ccacaggata gtctatcacg ctatcgtcca
     1681 accgaatcca gccgacgatg tccggcatgg tctgacgcag cttggcaaag tccttttcgg
     1741 agacggcctg ttggggctgt tcggactcca cgtccagata agagggcgga agcgtggcct
     1801 gcggcaccca ctgtgcgtct gcggtggacg gcgtggccgt ggggcccggc aggacggtcg
     1861 ccagcgtggg aaggctgacc tctccgccgt cgtctgccgt cccttcactg ttccgggaca
     1921 gggaagcnnn nnnnnnntaa agttcaagca gcgccagcgt acccagcacc agcgcggcca
     1981 ggatcagcac tgttagaaga aaaacaagca gggcgcgacg tgcgcccccc tgtttttttc
     2041 gatttttggg ataacggcgg ctcattgcgc accgccctcc ttcttgcgga gggaacggaa
     2101 aacgaatgcg gcggcaatca gcagcgccgc gcatccggcc acaagccacg gccagttttg
     2161 gctgacgccg gtgggggtca gcgtgccggg cttactggag tatttgttga cgaagagcgc
     2221 cagatcagtc ttggcttctg aaccgttcag ccgcatggcg acagcggtct cgatcccgtt
     2281 ttcgccgtta aaaacactga ccgtcacgtc ataggtgcgc cggtcatacg tcagattggg
     2341 gttgacgttc ttcgggatct gcgtgatcgt ataatgataa acgcccagct tcgtgaaggt
     2401 gatggggaac accacgtcca cctcacgcgc atagcctacg gc
//
LOCUS       DAWAEA010000206         2438 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6166_length_2438_cov_2.399496, whole genome shotgun sequence.
ACCESSION   DAWAEA010000206 DAWAEA010000000
VERSION     DAWAEA010000206.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6166_length_2438_cov_2.399496"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2155..2164
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          468 a          742 c          780 g          438 t
ORIGIN      
        1 cgagcgcggc acgctcctgt ccggcgggca gaagcagcgc atctccattg cccgggtgtt
       61 cctgaaaaat ccgcccatcc tgattctgga cgaagccacc agcgcgctgg acaacgaaag
      121 cgaacgggtg gtgcaggatt cgctggaaaa gctcagcaag ggccgcacca ccttcaccat
      181 cgcccaccgg ctgaccacca tccgcggggc gacctccatc tgggtactga cccaggacgg
      241 cattgtggaa aagggcaccc atcaggaatt gatggcgaaa aaaggcaagt attacgaact
      301 gtacagcatg tatacggagg atctttaaga tgacagaaat aaaggagcag aaccgctttc
      361 ttcccccgat gcggatgcat ccggcttacc gccacggcga tatgaccccc tggggcggcg
      421 aacagctgaa aacggtcttc ggcaaggaca tccctgacga gcgcaccggc gaagcgctgg
      481 aaatgagcgt gattcccgga ctggaaagca cggatgacga cggcgtgacc ctgacggtgc
      541 tgatcgagcg ctacggctcc cggctgacgg gtctgccgga aggcacggaa tttcccctgc
      601 tgctgaagct gctggcggcc aaaggcatcc tgagcgtaca ggtccatccg gacgacgaat
      661 acgccaaagc gcacgagaac aagctgggca agaccgaagc atgggtgatc cttcatgccg
      721 aggagggcgc gtcccttctg tacggcatcc gggagggcgt gaccatcgac gagctgcgcc
      781 gggccctgaa tggcggcgag gacgtggagc cgctgatcca gcgggtttcg gtcaaggcgg
      841 gcgacgtgtt ctatatgccg gcgggcatgg tgcatgccat cggcggaggc attctgcttt
      901 acgaaattca gcagtccagc gacgtgacct accgtctgtg ggactttaac cgggtcaacg
      961 ccaagggtga aaagcgaccc ctgcacattc agcagtcgct ggacgtgatc cgccctgagc
     1021 tgaagggcct gcgggcccat atgccggagc atccggcgca ggagatcacc cggctgctgg
     1081 acgtgcccgc ctttacgctg gactgcgtgg ccgcggacgg cgcctgcatc ctgcctgcgg
     1141 cggagacctt ccgggtgctg accgcgctgg agcctctgac tctgcgctgg ctggagggtg
     1201 agatggaact gaaggcggga gacacggtgc tgctgcctgc cgcctgcccg gaggttggac
     1261 tggaaggcca tggccgggcg ctggtctccg gcgttgggag cagggcatga gcaagttcta
     1321 caacggtttg atccgtctgg tgcgcttcgt tctgccggat atggaaacgg tctgggagga
     1381 acctttcgac ggcgaagcgg cggtcttctg ccccaatcac gccgcctcct tcggccctat
     1441 cgctatgtgc gcttttttct ctctgcggga tcagtgccac ccatggctga acgcgggcat
     1501 catggagcgt aaggagatcc ccgcctatgt ccggcaggac tactggtggg agccgggctg
     1561 ctttttcgag ccgctgctca atatgaccct tccctacctg atggctctgc tgctgccgcc
     1621 cattctgggc agcgtgcccg gcgtgccggt ctatcacgac aaccgggtca tccgcacctt
     1681 ccgcaaaagc ctggaatacg tggcgcaggg agagcatctg gtcatctttt ccgagcagcc
     1741 cgccggtcac ggcagcagcg aaatggagct gaaccggggc ttcctgcagg tggcccccct
     1801 gatctgtaag cgcacgggca aggcgctgaa gttctacccc gtccacgtcg accgggcggc
     1861 gcggcgcatc accgtctcca aacccgtgcc ctacgacccc gccctgcccc tgaaggagca
     1921 ggaggacggc atcattcagg cgctttccaa gagcattctg ggcaaatgat cgtgcaatga
     1981 taacagacga gccgctcttg atcgaacggc ccgttcctgc ttcatcatct cggcggagtg
     2041 ctgctggggt gtaggacggc gaaaaaacgc cggatggatg ccgaggggcg cgcccgacgc
     2101 ttttccctct tttttctgtg ctggccgtcg agggtcaagc agctccgtca agccnnnnnn
     2161 nnnntcccgt tcccccgccg aactctctgc gcggcggggg atgaccggca aagatacttc
     2221 tccacccaat cccatccccg aaaggagtcc tccccgatgg ttcaagtcgt agccgcgctg
     2281 atctggcgcg ataaccgctt catgatctgc cagcgtccgg aatataaggc ccgcgggctt
     2341 ctgtgggagt ttgtgggcgg aaaggtggag ccgggcgaga ccaaagccca ggcccttgcc
     2401 cgggaatgcc gggaggagct ggacgtgcag gtggaggt
//
LOCUS       DAWAEA010000207         2400 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6263_length_2400_cov_2.243497, whole genome shotgun sequence.
ACCESSION   DAWAEA010000207 DAWAEA010000000
VERSION     DAWAEA010000207.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6263_length_2400_cov_2.243497"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    221..230
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          474 a          702 c          797 g          417 t
ORIGIN      
        1 gctgggagtc cctttcatcg gtctgtacgg ggcctgctcc accatgagcg aaagcctttg
       61 cctggcaggg atgctgctgg acggcggtca tgcccagcag gtggtctgcg ccgccagcag
      121 ccatttctgc accgccgagc gccagtatcg ctatccgctg gaatttggct cccagcgtcc
      181 gcccacggcc cagtggaccg taaccggcgc aggagccacg nnnnnnnnnn cgccgccttt
      241 gtgggccgca aggaagggga ggggccgctg ggacactgct ttgaccatgt ggccaaggac
      301 gaaatgtacg ggcaggagag cttcgaactg gcggaacggc agctgtatct ggacgctatc
      361 gccatggcgg tcgagaaggg gcgggagcgg atcgagaacg tgcagtttct gctgggcggc
      421 gacctgctga accagatcat caccgcgtcc ttttcggccc gggagctggg agtccccttc
      481 atcggcctgt acggggcctg ctccaccatg gcgcccgccg cagccgatac ccttctttcc
      541 cttttccggg atacacagac cggcccggcg gactatgacg ctatcgtgac cggcgatctg
      601 gggcaggtgg gacacgacct gctgatgcag ttgatgcggg agcagagaat gccgttgatc
      661 gacgagcgat atcacgactg cggcctgctg atcttcgacc gggaacagca ggacgttcac
      721 gccgggggaa gcggctgcgg ctgcagcgcc gccgtgctga acgggtactt cctgcctaag
      781 ctgcaaagcg gcgagctggg gcggatcatc tttatggcca cgggcgcgct gatgagcact
      841 acctccagcc agcagggaga gaccatcccc ggcgtggctc acgcggtggt gctggaaagt
      901 ccgcaaaggg ggaagaagga atgaatatcg tctggatgta tctgaaggtg tttctggtgg
      961 gcggcgcact gtgcggcgtc ggcgagcttt tgaccctttt gacaaagtta accccggcca
     1021 gaattttggt ttgctacatt ctgaccgggg tggtgctcag cgcagtgggt tggtatgggc
     1081 cgctggtcaa gttcgccggg gccggggcga cggtgccgct gaccggcttt gggcacgcgc
     1141 tgtgtcaggg cgttctgttc agcgtgcagg aaagcgggct gatcggcgcg ttcaccggcg
     1201 ggctggccgc cacggcgggc ggcatcagcg cggcggtgtt cttcggggcg ttggtagccc
     1261 tctgcttcaa gccccgttcc aagtagtcag cccttctgcc tgcgggtaaa cagacggaga
     1321 aatccgctca ggaaacgggg tgcgcggacg cagatcatct tcacagccat gctttcacct
     1381 ccatgcccgg ctgattttga gcaggagaac acccagcgcc atcagcccga cgcccagaag
     1441 cgccagccag gcccagcagg gaatgcagca gaacaggagg atcacgcccg ccagcacgag
     1501 cgcatagccg agaatcattg cccctttgtt ttctccgccg gggtagcagc gttcataggg
     1561 tttcgccatg gcagttcacc tcgctcgttc cagcatatga cgagcgaggc gaaaatgtgc
     1621 tatttgcttt gctgaaattg ccgcagcacc gtcagcgtga ccgggacgga cagcagaaag
     1681 gagagcaggt cgctgacggc ctgactgagt tcaagacccg tcaaaccgaa gaaatgggac
     1741 agcaggaaca ccgccgggat gagaaaaagc ccctgtcggg cggcggccag cagcgtggcc
     1801 cggcctgcgt agccgatggt ctgcagcatc atgttggcca tgatgatgaa ggaaaacagg
     1861 ggcatggtga cgcactgcat ccgcagagcc gcggcgccga tctcccgcac gtccgggttg
     1921 tccttgatga acagcccgac gatctggggg gcgaaaatca tggcgacgat ggccacgacg
     1981 cacagcacga tggcggacag ccgcacacag aaccagaatg cgtcccgaac ccgtcggtaa
     2041 agccctgcgc cgaaattaaa gccgcacacg ggctggaagc cctgcccgaa gccgatgatg
     2101 gcggagttgc agatttgcat gatccggctg acgatgctca tggccgcgac cgccgcgtcg
     2161 ccgaagccgg gccagcgagg gaacgccgcc ccggaaaatt tctttggcag tctccgcgtt
     2221 caggcagaag cggcggggct tgatgggaat attgcccgca cggctggtca tgaacagaag
     2281 caccgcaaaa ctgacggcct gtccaatggc cgtggcgata gctgcgcccg ccacctgcat
     2341 gtccagcacg aaaataaaga cggggtccag aatgatattg aggatggtgc cagcggtaat
//
LOCUS       DAWAEA010000208         2382 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6314_length_2382_cov_1.770520, whole genome shotgun sequence.
ACCESSION   DAWAEA010000208 DAWAEA010000000
VERSION     DAWAEA010000208.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6314_length_2382_cov_1.770520"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1472..1570
                     /estimated_length=99
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2017..2038
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          519 a          625 c          673 g          444 t
ORIGIN      
        1 gatcagcttc gccctgtgtc tggataaaga cggccaggtg acgcaaatga tcaatattca
       61 acagccatcc gaaaaaggaa aggcgctgat gccgcagact atcagaaatt ttcctgcacc
      121 ggtaaagcgc accgttggca ttgcttccaa cttcctgtgg gacaactcaa cctatctgct
      181 gggcgttgat caaaagggaa agccgaagcg cagccaggat tgctttcgcg ctgccgcaag
      241 tctgcaccat accgtattgg atggcgtgga ttcgcccatt gccaaggcga tcctgacttt
      301 cttcgacacc tggcagccgg aaaaagcgat ggaacatccc gccttgtccg gacagtatga
      361 ggcagttacc gcaggcggga atctgctgct tcgggtggac ggccgctatt cacatgagga
      421 taccgccatc cgccaggcgt ggcagcggta tcgggacggc gcaggcgagg acgccgtgcg
      481 gatgcagtgc cttgtcacag gccgcgagga tgagattgcc gccacccatc ccaccatcaa
      541 gggtgtacgg gatgcccagt ccagcggcgc aacgctggtg gggttcaatg cgcacgcctt
      601 ttgctcttat gggcgggagc aaagctacaa cgcaccggta ggcaagtacg ccgcatttgc
      661 ctacactgcc gccttgaacc atctgctggc gaaaaggaac aatgtgcagg tcatcggcga
      721 taccaccgtc gtctgttggg cagagggcgc agagcctgct tatcctgatc tgtttatggc
      781 gttcctgtcc ggcaatatgc catcaggctt atcggaaaag gatctgcagg ctgccttgaa
      841 aaatctggca aagggtctgc cgagcacgga cctgggcgtg gatccggacc gcacctttta
      901 cattctgggg ctggcgccta acgcggcccg gctctctgtc cgattctttc tgcgggacag
      961 cttcggcagg ctgatgcaga acgtcaatgc ccactatgag cggctggaga tcgtaaacgc
     1021 taagcccggt gtgctgtccc tgtgggcgct gctgcgggaa acggtgaatc cgaattccaa
     1081 ggataagtct ccttcaccgg ccatggcagg agacacggca cgggccatct tttccggcgg
     1141 gctgtatccg gcgtcgctgc tggaggctgt catgctccgt attcgtgcgg agcggaacat
     1201 cacatggggc cgcgcggcca tcatcaaggc gtattatctg aaaaatccac atacggactg
     1261 tccaaaggag gttttgactg tgagtctcaa cgaagccagc accaacattc cttacacgct
     1321 gggccggttg ttctccgttt atgaatcggc gcagcaggcg gcaaatcccg gtatcaacgc
     1381 gaccatcaag gacaagtatt tcaactcggc ttgcgccacc ccggcggctg tgttcccgat
     1441 tctgctgaag ctggcaaaca gtcatctgaa annnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn gtgttgaagg gattgctggg cgagagctat cctgcccgta tgacgctggc
     1621 ccagcagggc tccttcgacc ttggctacta tcaccagaca cagaagcggt atacgaaaaa
     1681 ggaggagaat gaaaatgtct gagcctatca agaaccggta tgaatttgtg gtgtttttcg
     1741 atgtagagaa cggaaatccc aacggtgacc ccgacgcggg caacatgccc cgtatagacc
     1801 cagagaccgg ctacgggctg gtgacggacg tctgcctgaa gcgaaagatc cgcaactatg
     1861 tggagatggc aaagggaggg aagacgggct atcgcatcta catcaaggac ggcgtaccgc
     1921 tgaacaccag cgacaaggaa gcctgcgcct ttgtgggcgt ggacgcggac aagctgaagg
     1981 atgccaagaa gaaggacgag catctggacg aaaagcnnnn nnnnnnnnnn nnnnnnnntg
     2041 ccggaatttc tacgatatcc gcaccttcgg cgcagtcatg actaccttcg tcaagggtgc
     2101 gctgaactgc ggacaggtgc gcgggccggt gcagctgggc tttgcccgca gcgttgaccc
     2161 catcatgccg caggaggtta ccatcacccg tgtggccatc accacggagg cggacgcgga
     2221 gaagaagggc accgaaatgg gccgcaagta cattgtaccc tacggcctgt accgggtgga
     2281 gggatatatc tccgccaacc tggcccgcaa gactaccgga ttctccgagg atgacctgaa
     2341 cctgctgtgg acagccatcc tgaatatgtt tgaaaatgac aa
//
LOCUS       DAWAEA010000209         2381 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6319_length_2381_cov_1.599312, whole genome shotgun sequence.
ACCESSION   DAWAEA010000209 DAWAEA010000000
VERSION     DAWAEA010000209.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6319_length_2381_cov_1.599312"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1192..1263
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2122..2230
                     /estimated_length=109
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          446 a          630 c          657 g          467 t
ORIGIN      
        1 ggtttacccc gtgtatgtgg attccaatgc catggcgggc cgagcgggaa cctacgttca
       61 tgaccgctgg gaccggctgt gctatctgcc ctgcacggcg gagaacggct tcgtgccccc
      121 gctgcccgat cggccggtgg atgtgatcta cctctgctac cccaataacc ccacgggtac
      181 ggtgctgacc aaagagcagc tgaagccctt tgtggattat gccaaggcca acggagccat
      241 catcgtgtac gacgcggcct acaaggcctt catcaccagc ggcgcacccc ggagcattta
      301 cgaggtggag ggcgcgaagg atgtagccat cgagtgctgc agctactcca aaaccgccgg
      361 cttcaccggc acccgctgcg gcttcatggt catcccccac ggggtgaagg gcattggggg
      421 acagggccag ccggtggagc tgaacgccat gtggaagcgc cgcatgggca gcaagttcaa
      481 cggggtcagc tatcccatcc agcgggccgc cgaggcggtt tattccagag acggctgggc
      541 tcagtgcatg gacagcatcc gctactatct gcgcaatgcg caggtgatcc gggaagggct
      601 ggaaaaggcg ggctacaccg tgttcggcgg cgtggacgcg ccctatatct ggatgaagac
      661 ccccgacggc atggccagct gggatttctt cgacctgctg ctgaaccgct accatgtggt
      721 cggcacgccc ggcgcgggct tcggcccctg cggcgaaggt tacttccgtc tgacggcctt
      781 caatacgctt tttcatgtgg aaggccatag aagatgcgag gtcaagccgc tttttcagtc
      841 agacggagca gacagaccgg ctcggtgaag tccagcagga aggagatctg gccgtttttt
      901 tcctgcacag gcagtggagt ttccgcgcct tcggcattca gcagtgcaaa gccgtagttt
      961 tggaaagact cggcaggcag cgtgatcttc atctgcccat ttgcctgaga cagacggccc
     1021 gccgtttcaa acgcgacgct tagcagcaaa gtatcgtccg ccccttcgcc catgcgtacc
     1081 accgcttccc cggcgggcag gcgatcgttc atgacggcga cagagctgtt ttccacaagc
     1141 gccagctgcg ctccgccgct tcccttgccg caaactgggc agagctggaa gnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnncggtgaa gctttcgctg cagcgggaac aggtggaaac cgtatagccg tcggcggtgc
     1321 aggtcggcgc gaaggtctgg gacacatatt cgtgacccag cgccggagcc tccgaaaagg
     1381 tggtgtagtc gcagcgggtg cagtcctcat aggcgttcca gcccgcgtcg atgcaggtag
     1441 cgtccagtcc ctcatgctga accagatcgt ggttcagcgg cgcgatcgac cagtaggtgt
     1501 tgagatcgcc gcgagtacag accttatagg ggtgccagcc cttctctgtg caggtggggt
     1561 ccttcccctc atattcaaca aaatcgtgcc ccagcgcatt cagctcccga tagggctttg
     1621 tggaatagtc acagtaggca catttgttat aggcatccca gcctttgtct atacaggtga
     1681 cctcccgagc cggatagttt cgcagcaaat gggtacccaa tccaccgaag gatgaattac
     1741 atttgccgca tttcgccggc tgctggcagg ttgccgattg aacgatttca tgataggcaa
     1801 aataggtgaa gccacagctc tgacattcgg cttggtgcca ttgatcgtct gtataacggt
     1861 aagtataagc tttcccagtg cattcgccgt tgggattgcg gcacgccagc gctgtgctgc
     1921 ataaaagggt cattaacagc gccagtccaa gcgcaaacca gaatttttgg gggattctaa
     1981 ttgctgaaaa aaggcgggct tccgagccgg tgtgcagttg cttcatcctt gtttcctccc
     2041 tgtcggtcga gttggttcgt tttcagatag gggatcgttg tacggggtaa agcgagtttg
     2101 cgctggcagt ctgtcatcgt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn ctctttcact gacctttaca aaaatacgaa aatgcaaaaa agaagcgtcc
     2281 gaagacgctt cttttagact gtcgaaaaac cctgattggc tgccatcgcc cgcgaacagc
     2341 gctctgcggc ccgctcccct acggggcgcg ggcttcggac t
//
LOCUS       DAWAEA010000210         2373 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6332_length_2373_cov_2.717429, whole genome shotgun sequence.
ACCESSION   DAWAEA010000210 DAWAEA010000000
VERSION     DAWAEA010000210.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6332_length_2373_cov_2.717429"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          547 a          757 c          718 g          351 t
ORIGIN      
        1 acatcaacgg tcaggccacg gtggaaaaca ccctgtttga cagctgccgc gtgatgcttg
       61 gctccgacga cgtgggcatc gtgaaggact ccgacgtgcg gcacatcacc gccgccgagg
      121 cgcaggccct gatcgaccag tggaaccggg aacatcccgc cacgccggat cagcccacca
      181 cctccgtagc gggctattac atcaccacgg cggagggcgt gccgctgcgc tacggcccca
      241 acgcctatac ggccaccatc gcccagctgc cgctggacag cgtggtctac gtcacccagc
      301 agcagacggt gaacggcgtg gactggtatg tggcttacta caacggcaac gcgggctaca
      361 tccgcgccga ccagatgctc cgcaagatga ctgaagcgga aatcaacaag tatatggccg
      421 gtcagaaaac gactgcgcct acctcgccca gcaagacggc cgcgccttac gacccctacg
      481 gaaagtccag ctacggctat gtaaccagca attccgtcaa cttccgcgag acccccaacg
      541 gcaaaaagat gaagacgctg agcaagtatg ccttcgccta catcatcggc tccaaggagg
      601 tcaacggcgt tacctggtac tatgtcaacc agaatggcac catgggctgg attcacggag
      661 actacttcca tcagctgaat ctgaccgagc tgaccagctt cctgaacagc aatgaatacc
      721 ggcaggggct ggccaacaac tccagcagta cgagcagcag caccaacaag gacaatacgt
      781 ccggcaacac ctcggtgaag cccagcggcg gcaattccgg ctccgccacg gcggggaata
      841 tctcctctgt ggaggactgg aacgtgggca cgtggaagaa caccggcgtg accacgacca
      901 ccagctatgc gcccttcaat ccctatgcga ccgccacccc cgcgccctcg gccagcgtat
      961 cgccgtccgc cagcgcggag ccgacggaga ccttcgtcat cggcacgatg atccccatta
     1021 cctatgacga cgaaagcaag gagacccaga ccggctcggt gccgtggggc ctgatcggcg
     1081 tgggcgtggt actgatcggc ggcgcgggcg gtgcctatgc ctacgcggtc aaccagaaca
     1141 agaagcgccg tgcggctgcg gcccgtgcag cggccagccg ccgtgcagcc ggaaccggca
     1201 cgcagacggg cgcatcccag agcggcagca gccaacaggc acagaatccc tacgcccgcc
     1261 gggctgtggc ggctccgccg gtaacaggcg cgggcaccca gcagcggcag cagaaccaga
     1321 atccgacggg aacgacgaat ccctacgctc gtcccagcac gccctacgga acccagaata
     1381 atggttcttc ctatggagcg aacggatcga atccgagcgc agtaaacgga acgggcagcg
     1441 cggcgaatcc ttacggcggc acttccgcct atggcagcac caatccgtac agcccgtcca
     1501 acaatcccta cagcaacggc agcattgtgg gcagcgagac aaagtcctcc tacggaacgg
     1561 agacccagcg ctcgtccttt gccccggctt ctcagcagcc cgaggaacgt ccggccgcca
     1621 cggccggaac gcccgcgcag cggacgaacc cctacgcccg gcccattgaa cccgtctccg
     1681 gtgggacgac gcagtccggc cagccgcagg ctttctccac tggcagcgag gagccccgcc
     1741 gccggtctac ccggatgcag cgctaccacg ccgcggaaga ggaagaccag aacaaataaa
     1801 ggatcctgat cccaaaggga cgccgtgaaa atggcgtcct ttttgattag agaatagggg
     1861 aaaagcgtca aagtcttatt ttaaggggag ctcgtgttga tttcggcagc gatggacttt
     1921 tggatgatta cgtttagaaa ttttgaaggc ccctctgcaa gctgcggggc aagccaattt
     1981 tgcagtaaaa ataccccggt ggggattgac gtgccgacta cggcgcgggg gtggaacttg
     2041 gcgtatgggc ttttttcggt aggagaaacc gaaggggtgc aggggggaac ggagggaaat
     2101 atcagcccag tgggctgtcc gccgaaggcg gaaatgaccc ggagttcagg caaacaacaa
     2161 gcggagcgca cggcagtgcg ggccgcgccg attgtttgcc ccgaggaaag ccccctgctt
     2221 gtagcagcac acggcaactt tgctgtaaga aaagccctcg ccggggcttg acgcgccgac
     2281 tccggcgcgg atggtggggc tgccgccccg aagggggaag tactttcttt cggcgcgaaa
     2341 gaaagtacca aagaaagcac gcggcacggc gac
//
LOCUS       DAWAEA010000211         2331 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6457_length_2331_cov_2.074253, whole genome shotgun sequence.
ACCESSION   DAWAEA010000211 DAWAEA010000000
VERSION     DAWAEA010000211.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6457_length_2331_cov_2.074253"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          371 a          756 c          677 g          527 t
ORIGIN      
        1 ctgctgggca tgtgcaccaa gtttgccgag gtcacactgg ccgtacattt ccgggaacgg
       61 aaccagcacg gcgactacgt aggcgggccc atgtactata ttcagaacgg tctgggccgg
      121 aaatggcgct ggctggccta tgcctatgcc ctgctgggcg cgctggcctg cttcggcatc
      181 ggcaacacca cccagatcaa caccatcacc gtggccgtgg gttccgtgct gaccagcttc
      241 aacctgatcg gcgagacggc gctccccacc ttccggtgga tcttcggcgg gctgatggcc
      301 ctgcttctgc tgatcgtcct gctgggtggc gtgaagcgca tcggcaacgt ggcggaaaag
      361 ctggtgccct tcatggcggt gttctatatc gtgctggcgc tgggcgtggt gttcagccat
      421 ctggaccgga ttccgctggt gttcgaacag atcttcgcag gtgctttcaa tccgtcggcc
      481 tttaccggcg gcgtggtggg cagcttcttc ctaagcatga agaaaggcgt ttcccggggc
      541 atcttctcca acgaggccgg cctaggcaca ggctctatcg cccatgcctg tgccgagacc
      601 gaccacccgg tccatcaggg ttatttcggt atcttcgagg tgttcgccga caccatcgtc
      661 atctgcactc tgacggcgct ggtcattctg gcaagcggtg tgcccattca gttcggtcag
      721 gcggccggcg cggagctgac catcagcggc tttgtggcca cctatggcag ttgggtgacc
      781 atcttcaccg ccatcgccct gtgcagcttc gccttttcca ccgtgctggg ctgggggctg
      841 tacggcgcgc gcttcgtgga atacctttgc tcctcccata aggcggtcaa gccctttatg
      901 gtggtgtttt ccctggcggc cattctgggc gccacgatgg atctggggct gatctgggat
      961 ctgagcgata ccttcaacgg cctgatgtcc ctgcccaatc tggtgggcct gttcctgctg
     1021 agcggcacgc tgttcaagct gctgcgggaa cgggccaaag cggacgagac ctcgccgctg
     1081 gccgctcggg attaaaaccc aaaagcgcct gccgcagtta ggagcggcag gcgctttttt
     1141 atacctgatt catttgttcc agtatgcgtc ggcgcgtctg ctgaagccgg tccatcacct
     1201 gcatcagctg ctcgtcggtg cgttccaccc gctccttggc atcgctgatc ttgctcagta
     1261 ccgtccaatc gctgatgaac ccgtcccaca ccagatcggc cacacgccag aagccggaca
     1321 gctccgccga ggcgctgcac tggacgcgca cgtccttcaa ctcccggttg aaccgttcca
     1381 gcgcctgacg caggctctcc aactgctgct gggccctgtc catgcgggag tgcttcatca
     1441 gcccggacag catcccgccg cccagcaggt cgtagattcc ccagcccctg gcgctgcgca
     1501 ggctgtccag cacctgctga gcctgccggt gggcttcctg cccggccccg atggcctcct
     1561 cggtttcccg aagaagagaa gcatagtcgc tcatagtctt gcccttattc ctccgcgccc
     1621 tccagaattt tcagcgtgga gctggtgccc agacggctgg cgcccgcctc caccatggcc
     1681 agcgcctcct gccaggagtg catgccgcct gcggccttga ccttcatgga cgcggggatg
     1741 gaagccttca tcaaagccac atccgccacg gtggccccgc cggtgccgaa gccggtgctg
     1801 gtcttcacat aatccgcgcc ggccttgacg caggcctgac aggccagctt cttttcctcg
     1861 tcggtcaggt agcagcactc aatgatgacc ttcaccagcg tgccgttcgc ggcttccacc
     1921 acgccccgga tgtccttttc cagcagttcc agattgccgc ttttcagcgc gcccagattg
     1981 atgaccatgt ccacctcgtc cgcgccgttg gcaatggcat cctttgtttc aaaggccttg
     2041 acggcggtgg tgttagctcc cagaggaaag ccgatcaccg tgcagacctt gaccgggcta
     2101 cccttcagct cttcggcggc caaagccacg tggcagggat tcacacaaac gctggccgtg
     2161 cccagctccc gggccgtggc gcacagctcc cgaatctgct cgggggtagc agtggccttc
     2221 agcaaagtgt ggtcaatcat agaggcgatc tgttgtttcg tcatatgttt tccttccttt
     2281 ctgtgtccat atggatgttg gatttttgtt tttccttgca tggtagttat g
//
LOCUS       DAWAEA010000212         2330 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6461_length_2330_cov_2.524835, whole genome shotgun sequence.
ACCESSION   DAWAEA010000212 DAWAEA010000000
VERSION     DAWAEA010000212.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6461_length_2330_cov_2.524835"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          450 a          732 c          727 g          421 t
ORIGIN      
        1 ggcctacggc tgcgccacct tcctcaaggt gctggacaag ctcagcaagc agtttggcag
       61 tctgcgcatc cgcagcgata attttaacgt gatggtgtcc gcagccgtgc ccatcagcaa
      121 ggaggccgcg ccccagctga gcaaggaaaa ctggcgcacc gttattcagg agcagcttca
      181 ccgctacaag gaggacggct tcgaccgcat taaccccagc atcctcaagg ccgacctgca
      241 ggccacctat cccgattttt ccgagcgggc gctgggcttc aagcgcttca gcgatatgct
      301 caaggcattg gaaaaggacg gcctgctcac cgtggagctg gacgagcagg gaaccatgct
      361 catcaacgtg ctgtaagcaa aaggagagat caacatgccc aaaggctttg cccgcgtcgc
      421 cgccgccagt ctgcccgtcc atctggggga cgttgcggcc aacgaacagg aaattctcag
      481 cgccatggag ccgctgcgcc gtcagggcgt gcagctggcc gtttttcccg aactgtgcct
      541 gacgggctac acgctgggcg atctgctgct gcagccgctg gtgcagacgg ccagctggga
      601 gagtatgctg cgcattgcgg aaaaaacgga cagttccatg gctgtggtca tcggtctgcc
      661 ggtgaacgtg cgcagccgcc tgtacaactg cgctgccgtg ctctatggcg gacggcccat
      721 cggctttgta cccaagacct atctgcccaa cggcaatgaa ttttacgaga cccgctggtt
      781 cgtcagcggt gcggaggcgg acgaaacgct ttcctgcacc ctgcccggcg gggaagtctg
      841 ttccgccctg cttcggcccg atcagctctt tgatctgggc ggattcacct tcggcgtgga
      901 gatttgcgag gatctgtgga cgcccattcc gcccagctgc cagctggcgg tggatggggc
      961 ggacgtgatc gtcaacccca gcgcctccaa cgcgctggtc agcaagcacc agtaccgccg
     1021 tgagctgctg agtcagcagt ccggccgtct ctacgcaggc tacgcctacg cgggcgcggg
     1081 ttttggcgaa agcaccagcg atctggtttt tgacggctac accgccgttt acgaaagcgg
     1141 acgtgccttg agcgaggggc tgcgcttcca gcgggagggc ggcacggcca ttgcggatat
     1201 cgacgtgcag cggcttcgct atcagcggca gcgcaacggc agcttccacc agatgccggc
     1261 cggaaagcgc aagcccgcct ttgtgagcga cacgcctgtg gcggaggcgg cgctgccgct
     1321 catccgccgg ttggacccgc tgccctttgt gccccaaggt acggacgcgg atggacggct
     1381 ggaggagatc gtccacattc agatgaccgg cctgatgagc cgtcttgcgt ccattcactg
     1441 caagcatctg gtggtgggcg tttccggcgg attggacagc acgcttgccc tgctggtggc
     1501 cgcgagggcc tacgacgagc tggggcttga ccgcagcggc attcacgcca ttaccatgcc
     1561 cggcatgggc accggcgcgc gcaccaagtc caacgcggac attctgatgg agcgtctgcg
     1621 cgtcagttcc agcgagatcg ccatcggccc ggcggtgaaa cagcattttg ccgacatcgg
     1681 gcaggacgag aacgtccacg acgtgaccta cgaaaacagt caggcccggg agcgcacgca
     1741 gatcctcatg gactatgcca acaaagtggg cggcatcgtg ctgggcaccg gcgacctgag
     1801 cgaagcggcg ctgggcttct gcacctacaa tggcgaccac atgagcatgt acaatgtgaa
     1861 ttgctccgtg cccaagacac tggtgaaaag tctggtgcgg tacatcagca cgcattcctt
     1921 tgacgggcag gtaacggacg tctgccagga catcattgat acgccgatca gcccggagct
     1981 gctgcccgtc agcgagggcg agctgaacca gcgcaccgag gatattctgg gcgactacgc
     2041 cctgcacgat ttcttcctct atcacatgat ggacagcggc gcttccccgg aaaagctgcg
     2101 tctgctggcg attcaggcgt tcgagggcgt atacgccgct gaaaagatcg acagccagtt
     2161 ggagaccttc ctacgccgtt tcttccagca gcaattcaaa cggaactgcg ttcctgacgg
     2221 ccccaaggta ggctccgtca gtctcagccc ccgcggcgac tggcgtatgc ccagcgacgc
     2281 gaaccgcacc ctctggcagg gctaaagccc tgcaccccgg actgcgcggc
//
LOCUS       DAWAEA010000213         2327 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6473_length_2327_cov_1.835827, whole genome shotgun sequence.
ACCESSION   DAWAEA010000213 DAWAEA010000000
VERSION     DAWAEA010000213.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6473_length_2327_cov_1.835827"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          471 a          702 c          662 g          492 t
ORIGIN      
        1 gggtggaagc tcgtctgtgc ctgttcaagg gcgaaaacca cgagctgtcc cggggcggca
       61 agccgaagaa ccgcgtcaag cgtctgcagg agatcacccg ctggatggat cactacctgc
      121 ggccctgacc ccgcgaaaat caacgataca gaaaggaagc atttttcatg cgtattagcc
      181 tgttggagcc catcggcgtt accccggaac tgatcgaaga actgtccgag ccaatttgca
      241 aggccggtca cgaatttatc tactacccgg aaaagaccac cgatcccgct gaactgatcc
      301 gccgcagcat gggctgcgag gtggtcacca ttgccaataa tccctacccc gatcaggtgg
      361 ttctggccgc cgatcagctg aaaatgctga acgtggcctt caccggcatc gaccacgtgg
      421 ggctggaagc ctgccgcgcc aagggcgtgc agatctgcaa cgccgccaat tattccaacc
      481 agaccgtggc tgagctggtc atcggcatga ccatcgacct gctgcgccat atggacgagg
      541 cccagcagcg ggtgcatggc ggcggcactg ctgccgggct gaccggccgg gagatcatgg
      601 gcaagaccgt gggcatcatc ggcacgggcc gcatcggcat gatcactgcc aagctgttcc
      661 tggccttcgg cgcgaaggtc atcgcctact cccgcaccga aaagccggaa gccaaagcgc
      721 tgggcatcac ctatctgccg ctggccgacg taatggccca gagcgacatc gtcagcatcc
      781 acacccccaa caacgcctcc acccgcggac tcatcagcgc ggaaatgatc gccctgatga
      841 agcccaccgc gctgctgatc aactgcgccc gcggccccat cgtggacagc gccgctctgg
      901 ccgcggccct gaacgagggc cgtctggccg gtgctggcgt ggacgtgttc aatcaggagc
      961 cgcccatccc ggacagcgag cctctgctgc acgcccgcaa tgccctgctg actccccata
     1021 tcgcctttgc ttctcaggaa tccatgattc gccgcgcccg gatcgtcttc cagaatatcg
     1081 aagcctatct ggccgggaag ccggagaacg tctgccagta ctaagtcccc ttcttttcct
     1141 cccgttgaca cgccgtgcca acgggttttt tttatcaagt ttgacctata ttttactttc
     1201 tcagggtggc cgttttttct tttctctggt aaaatgattt ttgtatctgg aagaagcaag
     1261 agaagaaacg aggaatgaag atggcttatc agacgattgt gatcgattac gcgcccaagg
     1321 ccaagagcat ggccgccgaa gtcgagaaaa aggccaacga gatggagcag gaggactggg
     1381 agctggtgtc gttcgccatt accaactccg ccaaggggat tctggtcttt caccggccgg
     1441 ataacgattt tagagattaa cgcgtttcat gcagaaaagc ctgtgcctac ggacaggctt
     1501 tttttgcgcc attttttcgg taaacaggca tagtgctcct ttgctttcgt tttcttatgc
     1561 catttgtcca aatccgccgc tctgaaaaaa gagcggcggg tcaatatttg tttttcagcc
     1621 ctgcagcatc atctgatagg tgggcatggg ccaatagtcg gcccccacca gcgtttccag
     1681 catatccccg gcagcgcgca gctccgccat ggcgggcacg attcgatccc gggtggcctg
     1741 tgccttttcc agcgtgtcct gaaggggcgc gatctcgttc agagcgtttt gcagactgtc
     1801 gcagcccttc gtcagccgat ccatctgacg ctgaatgtcg gtcagcagcc gggtctcggt
     1861 ctccatgggc agcgtggggc aaacctgctt cttctggacg gcctcctgcg ccaccgtgcc
     1921 ggaataggcg atggcggcgg gcaggatcat tcgccgggca atatccagca tgctcagggc
     1981 ctcgatgtcg atgaccttga cgtagttttc cagcttgatc tcgtagcggc tgtgaacctc
     2041 ctgcgcgctg aagaccttct gccgctcgaa caaatccaga tttttctggt ggatgtaata
     2101 aggcagtgcg tccaccgtgc tggaaagatt cagcaggccc cggcgggcgg cttcctccac
     2161 ccaggacgcg tcgtagccgt tgccgttaaa gatgatgcgc atatgatccc gcagggtgcg
     2221 gcggatcagc tcatccacgg cggcggtgaa gttctccgcc tgttccagct catcggccat
     2281 gtgcatcagg gtgtcggcaa taatggtgtt cagcaccacg ttggggc
//
LOCUS       DAWAEA010000214         2324 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6483_length_2324_cov_1.280740, whole genome shotgun sequence.
ACCESSION   DAWAEA010000214 DAWAEA010000000
VERSION     DAWAEA010000214.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6483_length_2324_cov_1.280740"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    427..436
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1868..1877
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          483 a          647 c          712 g          462 t
ORIGIN      
        1 ggccgacgtg ctggtgggca acggcgaata taccttcacc aagatcggcg tagaggatca
       61 gttcggtcag agcggcaagg ccatggacgt gctgcgggcc tacgacctgt gcgcggatca
      121 gatcgccgct cgtatcgctg cgaagatctg atttttccaa aaagaaagaa accgggaggc
      181 tccaaaaggg gcttcccttt tttggtttga ggcaaataaa agcgccccga agcaccagcg
      241 cttcaggggc gaaaaagaga cgttatccat gcgaacggga atagatgccc gtctgtatct
      301 cccggatata ggcttccttt atgggcaggc catcggcggc ttccgcgtcc cggacggcct
      361 gttcccggtc gtaatccagc tggcgcagcc gaacttccag cggcgctgga atgcggcctt
      421 cttcgcnnnn nnnnnngcgt agcagcattt gggcacgccc atggcgttgc cgacgctgcc
      481 ggtgttgacg atgatcccgg gggacagggt gcgcatggcc tgacggtgcg cgtccgcata
      541 gagcagcccg tcatagggaa cgcccttcgc gtccaccaga aaggattgca gcagttcccg
      601 gtcgtccgcc acggtgtaca gctccggcat gacaggccgt ccgtggaaca agcggaaccg
      661 gcggccactg aaccagaatt ccgtctcctg gggcagctcc cggagacaac gcagtcgttc
      721 cgggcccagc tgatcccagt agtagtgatc tctgggaaaa gtcctgcatc caacgccgta
      781 gtcccagttc ccgccgatga tgagctggca gtgctcgaaa gcccactcat aggtaaaaag
      841 attgctgggc cccttaccga caatatctcc cagacagacg atccgatccg gcttcgtcag
      901 ggccagatcc cgttccagtg cttccgtggc gggacggttg ccgtgcagat cggccacaag
      961 ggcaatgcgc atcgtccatc ctcccttaca caatcattcc atggcagtat agcagatttt
     1021 tgcaccgcaa acaagtttca ttgtcataaa ctttttttca gccggagggc gtagagcttt
     1081 gcgaagtgga ggaggcgggt tccctgtccg tgccggacgg ccttagcggc ctgtatgccc
     1141 tgatgcagga ctgcaatccc tttgcgtccg cctatgtgtt tcggatgcca aaggggcggg
     1201 cgctgatgag cgtggcctgc accaccatcg gcaatcctaa gaccgcgctg gagctgctgg
     1261 agaactggcc acagattttg aacgctctgc agggagaagg caagtccgtg atgatcgaac
     1321 ccacctatcc ccgggtagag gcgcagtacg accgtcagac gctgcacgtt caggcgattc
     1381 tggaaacggc ggacggcagc ggcctgcgcg tccggctgga cggcgtagcc ttctatcagg
     1441 gaaatgacct gatcgagctg tggcaggcct ggccggaggc caccacctat cagctgatgc
     1501 cgctggccct gcaggaactg cgggatgatc tggccacgct gaaggaattg acagacagct
     1561 ttcagtttca ggattcaagc cagagtcagg aggaaagcct atgaagaccg ttagcgtgat
     1621 cgtgccctgc tataacgaag aagaagcagc gccgattttc tatcgggaat ttttgaagcg
     1681 cacggcggaa atggactgcc agtgggagtt ctggtttgta gacgacggca gcaaggacgg
     1741 cacgctggca gaatttcgcg ccctgcgggc gcaggacgag cgggtgcatt tcatcagctt
     1801 ttcccggaat ttcggcaagg aaagcgccat gtatgccggg ctggaggccg ccacgggcga
     1861 ttatgtgnnn nnnnnnnatt atgtggccat tatggatgta gacctgcagg atccgccgga
     1921 gctgctgccc ggaatgctgc agagcattga gaaagaaggc tacgactgtg tagccacccg
     1981 gcgcgtcacc cggaaggggg aaccgctcat ccgcagcttt ttcgcccggc agttttaccg
     2041 cctcatcaac cgcatctcca agacggagat cgtggacggc gcgcgggatt ttcgcctgat
     2101 gacccgccag atggtggaca gcattctcag tctgaaggaa gtcagccgtt tttctaaggg
     2161 gattttcagc tgggtgggct accgcaccaa gtggttggaa tatgaaaacg tggagcgggt
     2221 agcgggggag actaagtgga atttctggaa gctgttcctg tattccatcg acggcatcac
     2281 cgccttttcc tccgcgccgc tggcgatcgc gtccattacc ggcg
//
LOCUS       DAWAEA010000215         2322 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6486_length_2322_cov_1.742832, whole genome shotgun sequence.
ACCESSION   DAWAEA010000215 DAWAEA010000000
VERSION     DAWAEA010000215.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6486_length_2322_cov_1.742832"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    735..744
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          530 a          647 c          666 g          469 t
ORIGIN      
        1 catcggcagc ccccagatga acttcttcaa cgtgaccctg gagaaggaag gcaacgacgt
       61 tgtggccaag ttcggcgaca acaagatcac catccccacc agcaagctga gcaagttcac
      121 tgacgagagc tacatcggca aggaagtgat catgggcatc cgtcctgaga acatccatga
      181 cgaagacgtg ttcgtgcaga ccgccagcag cgccgtcatc aacgccaagg ttgaagtgac
      241 cgagctgatg ggctctgaga cctatctgta cctgtccacc agcggcaagg acgagaacat
      301 catcgctcgc gtcaaccccc gcaccaccag ccgcgccggt caggatgtgc aggtcgcctt
      361 cgacgtgaac cacctgcatt tcttcgacaa ggagaccgaa gtgaccctgc tgagccgcta
      421 aggtttgctg aaaatcgaat catcgccgcc gggagaaatc ccggcggttt ttctgtgctc
      481 ctttttgaaa aagctgtcct gctgatagga tgaacaaaaa acgaagatca atggttgaca
      541 atccgttata gaaatgataa tatcatcttg tggttaatca aaactaacta caatgcattt
      601 ttgacagaac gaatggaggc ggagcaatgg cacagcgcat tctgaagagg aaactggtgg
      661 gacttggcta cgccgcggct ctggtggccg tgggctggtt ggtaactaac gtcttgtaag
      721 ggcgaaaaag cgggnnnnnn nnnngcaagg gtgctgtgta cggcacgcct tgctctgctt
      781 atttttaagg agggtttcat catgagcagg tacagcaagg atgacatcat tcggatggtg
      841 catgaggacg aggtggagtt tatccgtatg cagtttaccg atatttttgg ccagctgaag
      901 aacgtggcca ttacggcttc tcagatcgag aaggcggtca ataatgagat catgatcgac
      961 ggcagctcca tcgaaggctt tgtgcgcatc caggaatccg accagtacct gcgtcctgat
     1021 ctggatactt tcaccgtact gccctggcgg ccccagcagg gcaaggtggc ccgcctgatc
     1081 tgcgatgtgt acaatcccga cggcacgccg ttcgtgggcg atccccgggg cgcgctgaag
     1141 cgggtgctga agaaggcggc tgatatgggc tacagcttca acgtaggccc ggagtgcgag
     1201 ttcttcctgt tccagacgga tgaagagggc aagcccacca ccgccaccaa cgatgaagcc
     1261 ggttactttg atctgggccc gctggatcac ggcgaaagca ctcgccggga aatctgcatg
     1321 acgctggaaa agatgggctt cgaaattgag gcttcccatc acgaagtggc cgccggtcag
     1381 catgagatcg acttcaagta cgcccccgcg ctggagtgcg ccgataagat catgaccttc
     1441 aagctggcgg tcaagacgct ggcccagaag aacggccttc atgccacctt catgcccaag
     1501 cccatcttcg gcatcaacgg cagcggtatg cataccaaca tgtccctgtt caaggacggc
     1561 aagaacgtgt tcttcgatga aaacggcgag aagaagctgt ctgccgatgc gtatcatttc
     1621 atcgcgggcc ttttgaccca cgtgagcagc ctgaatgcca tcctgaaccc gctggtcaac
     1681 tcctacaagc ggctggtgcc cggctatgaa gctccctgct ataccgcatg gtccgcttcc
     1741 aaccgttccg cactgatccg catccccgcc gcccgcggac aggcgacccg cgtggagctg
     1801 cgctgccccg atccctcctg caacccctat ctggcgctgg cagtgtgtct ggccgccggt
     1861 ctggacggca tcgagcgcaa gctgacgccg cccgagatca ccgagaacat cttcgccatg
     1921 acccccgccg agcgcgaagc caagggcatt cacagtctgc ccggaactct gcatgacgcg
     1981 gtagtggcgt tccagaagga taagctgatc tgcgacacgc tgggcgagca cattgtcagc
     2041 cagtatgtgg cgggcaagga aaaggaatgg gatgagtacc gcacccatgt cagcgattgg
     2101 gaactgggca agtacctgat cgcctactaa gaaatgcttt ccgttccttc cgccggggct
     2161 gccgaggacg aacgctccgg cggaaggaaa gccctgtttt gaatcttttt ggaataagga
     2221 gaggatgccc tatgagtcgg ctgatcgttg cctttgaatc ggaagtgacc aggcagcggg
     2281 tgtgcgagtt gtttgcggcc tcctcttttc ctgtgcgcgc ct
//
LOCUS       DAWAEA010000216         2308 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6517_length_2308_cov_1.711052, whole genome shotgun sequence.
ACCESSION   DAWAEA010000216 DAWAEA010000000
VERSION     DAWAEA010000216.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6517_length_2308_cov_1.711052"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    531..540
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1612..1621
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          515 a          649 c          675 g          449 t
ORIGIN      
        1 tcgacattcc tacccagctg agaaaggcag agcgcgctgc gttcccgccc ctgcaccgcg
       61 ctatcgtcta cccggtgcgg aagttcattt ttcggaaatc caagcagctt tccgctaaga
      121 aaggataaaa gccaaacgaa tggagatcag agaataccga cagtacgacg aacgggaaat
      181 cctgctcctt tacgccagtg tgggctggac ggcctatacc gatcagccgg aaacgctgcg
      241 caggggcttt gaaaattccc tgctgacgct ggccgcttac gaagaaaacc ggctgatcgg
      301 cgttcttcgg gctgtggggg acgggcagac gatcgttttt gtgcaggact tactgatcct
      361 gccggaatat cagcgcagag gcgtgggtac ggcgctgctt cgggagctgc tggctcgata
      421 cccgcaggta cggcagatcg aactgacggc ggacgacagc gcgtctcttc tggccgttta
      481 tcgaaagatg ggttttgtgc ccatgagcga gctgggctgc tgcggactga nnnnnnnnnn
      541 gcgtgggaat ggacatcaca ttgtagcggg ccgccacgtt tggcgcttcg tccacgttca
      601 gctttacgac cttgcagccg gatacctccc cggcgatctc atccaccacg ggactgagca
      661 tcttgcaggg gccgcaccag tcggcataaa aatcaaccag cacggtttcg gcgctctgca
      721 gcacttccgt ctgaaaattc tgttcgttta ctttggtcac agccatcgca ggttcctcct
      781 ttatcatcgc ccgccgaagc gggcctctgt taaagtatat ccgttcgaag cgccggtgaa
      841 accatatcgc cgccgcttcc tttttagtat aagcgaagcg gcggcttcat gcaaacattt
      901 ttccgcagaa acgacaaaac ctcgcatttc agcaaagaat tgtcagggta ttttccctgc
      961 gcaaaggcaa aatagcgttg aacccacatc aaggaggtga aaaaaagatg gcaaaacaga
     1021 gcggttcctc ccggaacacc cagaccaccg tgcccgaagc gcgcgccgcg ctggataaca
     1081 tgaagtatga gatcgcccgg gaactgggca tcaacttcaa tcagggctac aacggcgacc
     1141 tgagctcccg ggaaaacggc tacgtgggag gctatatggt gaagcgcctg atcgaacagg
     1201 cggagcggca gatggcttct cagggcggaa agacctcccg atgaacggga aatatttgaa
     1261 tgaggtgaag ggaaatgtat cagaatcagc agagcggcac caacaacggc ggcatggtcc
     1321 ccgaggccaa gcaggccctg aacaacatga agttcgagat tgccaacgaa ctgggcatca
     1381 atctcaagca gggctataac ggcgatatgc ccagccgtca ggccggttat atcggcggtt
     1441 acatggtcaa gcgcctgatc gaacaggccg aacgcgccat gagcggcagc cagaagtaag
     1501 cttctgaaaa aagagaggcg gcacgatgaa cgcatcgcgc cgtctctttt ggtttgtgga
     1561 gaatcattcc tttgactttt cagcgctttc ctgcgtggcc agccatttgc gnnnnnnnnn
     1621 ngacgaagac tcgcacaaag cttttggcaa agccgtcctg attcagatag cccagctgct
     1681 cggcgatctc gccgcttttg cggctcttgc gaagctcgcc agacgcccac tgcatctgca
     1741 cgccctgcac atagtccgtg aaagttttgc cggtctgctg gtggaactgg cggctgaaat
     1801 aggaatagtt caggtgcatc tgggcgcaca gctcccgcag atggacggga cggcccgggt
     1861 tggactgaac gtagctgacc gcccagagca ccgggttgtt ctgcttgagc atctgatcgt
     1921 tggtcatggc ctcggaatag aacgcggtgc ggctctgacc gtccagccgg gcgatctgca
     1981 gggcgcgtcc ggcctgacgg agccgttccg ccgtgagcgg cccgggcagg ctggcaccga
     2041 tccagacgtt ttgcccggcg caccattgct caaagaccgt gtgctcgtcg gaaaaaagca
     2101 tcagctcgcc gccgagggtc agataagacc agcggaaggg cagcaccgta tcccttggca
     2161 gtgggggaga gagccgaacc gcctgaacga cggcgggagg gcggtgcata ccggcttttt
     2221 cgaaccactc cggggcgatc tccgaaggct cctgcgccag cagggccttt tccagcttca
     2281 tccggcccag cagccattgg ttttccaa
//
LOCUS       DAWAEA010000217         2290 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6577_length_2290_cov_1.941387, whole genome shotgun sequence.
ACCESSION   DAWAEA010000217 DAWAEA010000000
VERSION     DAWAEA010000217.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6577_length_2290_cov_1.941387"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    252..382
                     /estimated_length=131
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          389 a          675 c          653 g          442 t
ORIGIN      
        1 gaattgcttg gaggagcagg gtttctactg tgcctttggg gtcatcgact cccagaacga
       61 ggccagtctc gccctgcacc ggcgcttcgg ctttgtggag caccatcgca tggacaacgt
      121 ggccttcaag cacggcaaat ggctgaccac ggtctacatg cgcaagcagt tccgcgcgcc
      181 ggaaggcact cccgccgaga cgctgccctt cacggaagca ctgcgttcct atccccgatg
      241 actggtggca gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nncttttctt tcggtggcga aagaaaaggg tgcaaaagaa
      421 agccagcggc acggcgactc cagaggccgc cctgcccaga cgccgttggc gtggggcata
      481 ctcagccctt gtggcttggg atgctagggc gtgactggag catgtttgca acacaaaagg
      541 ccagcgtggg tttgatttca cgctggcctt tattgcgagc catgaggcct gtcgccgaat
      601 ggcggtcttt actatacaat ttgcagggag ccgcaccaga ccggcgagcc gtggcacctg
      661 tcgtgccccc gagcagccct gcgcgccttt ggcgcgcagt ccggggtgca ggggtagtac
      721 ccctgcccta gttgcggagg ctgtggaggg gggcggggat gcggccgccg cgctcgatga
      781 attcgtcgtt ggcagcagga ttcacgggca tgacgggggc atagccgaag aggccgccga
      841 agttgactac atcgccggcc ttcttgccgg gcgcgggaat gacccgaacc gccgtggttt
      901 tgttgttgac catgccgatg gccgcttcgt cggcaatgat gcccgcaatg gcattggcgc
      961 tggtgtcgcc ggggatggcg atcatgtcca gaccgacgga gcagacacag gtcatggcct
     1021 ccagcttttc aatggtcaga cacttggcct cggccgcttc gatcatgccg atgtcctcgc
     1081 tgacggggat aaacgcgccg ctcaggccgc ccacgtggct ggacgccatt acgccgcctt
     1141 tcttgaccat gtcattcagc atggccagtg cggccgtggt tccgggcgca cccaccatct
     1201 gtaggcccat ttcctgcagg atgtaggcca cgctgtcccc ccgggcgggc gtgggagcca
     1261 aagaaaggtc gacgatcccg tagggcacct gcagccgctt ggcggcttcc tgcgccacca
     1321 gttcgcctac gcgggtgatc ttgaaagccg tgcgcttgat ggtctccgcc agcacgtcga
     1381 agggctggcc gtgaatttcc tgcagggccc gctgcaccac gccgggcccg ctgacgccta
     1441 cgttgaccac gcactccggc tcgcccatgc cgtggaacgc tcccgccatg aagggattgt
     1501 cctccaccgc attggcaaat accacgatct tggcacagcc gaagccgtcg ctgtccgccg
     1561 tcagctcggc ggtcttttgg atgacatgcc ccatccggcg cactgcgtcc atgttgatgc
     1621 cgctgcgggt ggagcccacg ttcacgctgc cgcagagacg ctgggtatcc cggaaagcct
     1681 cgggcatgga gtccagcaaa atctcctccg cccgggtgga ccccttgtgc atcaacgcgc
     1741 cgtagccgcc gatcaggttg acccccaccg ccgcggcggc ctcgtccagt gccttggcga
     1801 tcagcacggg gttggtatcg gaaatcatgc tcaccggggt gacgctgatg cgcttgttca
     1861 caatgggaat gcccagttcc atctccagtg cttcgcccac aggcaccaga tttttcgcca
     1921 gccgggtcac tttttcgtaa acggcctccg ccgtctcccg gtcggtgttc tttttgcagc
     1981 tcagcagact gatgcccatc gtaatggtgc ggatgtcaaa atgctgctcg tgtatcatct
     2041 gcatggtctg cagaatttca ttgacttcga tcatgctgcc acctcagatc cgatgcatgg
     2101 cgtcgaaaat gtccgcccgc tgaatattga ccgcttcgcc cagttcgtcg cccacctgat
     2161 tgagacgagc ggaaatatcc gcaaaggaca cggacgcatt cgttacgtcc acgatcatca
     2221 acatggtaaa atagccgtcc agaatggtct gggaaatttc cagcacgttc acgttcaatt
     2281 cgtatagagc
//
LOCUS       DAWAEA010000218         2289 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6581_length_2289_cov_2.514324, whole genome shotgun sequence.
ACCESSION   DAWAEA010000218 DAWAEA010000000
VERSION     DAWAEA010000218.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6581_length_2289_cov_2.514324"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          425 a          716 c          670 g          478 t
ORIGIN      
        1 attttcaagg gctcgtccgc agccgatgac cacgttgttc tggggatcct ccgcgcagga
       61 gcagggcact ttcagcgtat cggcaatggc ttcgcacagg ccgaacagct ctgcgccgcc
      121 gcccgacagc aggatgccgt attcgaaaat gtccgccgcc agctcggcag gcgtatgttc
      181 cagcacgccg tggattcctt cgatcagctg ctgcagcgga tcgtccagcg cctcggtcac
      241 atcgtcgctg gtgatgcgca tggtcttagg caaaccggtg atcagattgc ggccggtcac
      301 ttccatataa agctgctccg accggggaat gacggatccg atgttgatct tcagttcctc
      361 cgccgtccgt tcgccgatga gcagattgtg cttgcgccgc agatggcgaa tgatcgcatc
      421 gtcgaagcgg tcgccgccca ccttgatgca gtcgctgacg atcaccttgc cgaaggacag
      481 caccgcgatg tccgtcacac cggcgctgat gtccacgatc atggtgccgt aggcctcgtc
      541 gataggcagg cccgcgccca gcgccgccgc cagcgaacgg ttcaacagtt gggtgcgtcg
      601 cattcccgcg tcaaacatgg tggagatcag cgagcggcgc tccatttcat ttacggcgct
      661 gggcacggcc agaatggccc ggggccgggc gaacatatgc ttgccgacgg ctttcatcac
      721 gaagcccttg agcatgaagc tgaccagctc gtagtccgta atgctgccgt cccgcaaggg
      781 ccgcagcgcc agaatattgc cgggggtacg gccgatcatc cggctggctt cctcgccgaa
      841 ggcgatgatc tcccggctgt cccggtcgat cgccaccacg gatgcctccc ggaggatgat
      901 ccccttgccc ttcatgtaaa tgagcacatg ggaggaaccc agatcaatgc caatatcatt
      961 gacgccagcc atcggtcatt accctttctt tctgcagaaa tgaaaacagg ccggaagggg
     1021 aattccgacc cctgcatttt cttattcgcc attcgattca tttttcctgc tttggtattt
     1081 ccggcgtgtc gcttcgccgc cccgcagatg cctcacggcc ttgttgtagc tcaggatacg
     1141 cctcacctgc gcggccagcg tctggctcag ctccggcagg cgctcgtgca tgtccttatg
     1201 tacggaagac ttgctgaccc caaaacgccg ggcggcggcg cgcaccgtcg ccccgtgctc
     1261 cgcgatatac tgcccgattt ccagacagcg tgtttcaatt tcttcccgca tgtccatccc
     1321 tccccggagg tttgggaaag gatatgcggc ctgggacgga aaattgcttt gttccctccc
     1381 ggggttgtct ttacaggaag aaaatgatac aatgctttcc atccccttta ccaaaggagc
     1441 ctttgttttg attaccttct gcaccctttt cagcggttcc agcggaaacg cctccgtgct
     1501 gcgcaccgaa agcggcgcgc tgctgatcga ctgcggcatg agcggcaagc aggtgctggc
     1561 cgccctgcag gcggcgcata tcgccccggc tgaactgcgg gccgtgctgg tgacccacga
     1621 gcacagcgac cacgtcaagg gcgtgggcgt gatctcccgg aaattgaacc tgccggtcta
     1681 tgccacggcg ggcacctggg agggcatgtc aggagccgtc ggggaaattt ccgccgctca
     1741 tcaggtgctg gtggagccag gggaaagctt tttcatgctg gacgggctgg aagtcaaccc
     1801 cttctccatc ccccatgacg ccaacgagcc tgtgggctac cgcatttatc tgcccggcgg
     1861 cagcagtatc gccgtagcca ccgacctggg ccatttttcc gaaaccgttc gagacgcggt
     1921 gctgggcgcc aatgtggtgc tgctggaaag caaccacgac cccgacctgc tctgcggtgg
     1981 aactgctcaa cgccggagcc cgtcacctgc tgctgggcca tctaagcagt gaaaacaaca
     2041 cgccggaaat agcttttcag acctcaaaag ccgcgttgga aaatgaagga gcccgcgtgg
     2101 gctcggacgt gacgctgcac gtggccagtc gttatcagcc cagcgtggtt tataccattc
     2161 gttgactcga tctgcatttc tggatgaagg agggatcagc cctgtctgtc accgtgctgt
     2221 gcgtcggcaa gctgcgggag cgcttttacg cggacgcggc gcaggagtat ctgaagcgcc
     2281 tttcccggc
//
LOCUS       DAWAEA010000219         2286 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6595_length_2286_cov_1.650381, whole genome shotgun sequence.
ACCESSION   DAWAEA010000219 DAWAEA010000000
VERSION     DAWAEA010000219.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6595_length_2286_cov_1.650381"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1507..1516
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          466 a          679 c          653 g          478 t
ORIGIN      
        1 ccaccgtcat ccagacctcc ggctccatgc ccggcctgaa ggccttcgtg gcggcggtgt
       61 tcggcggcat cggctccatt cccggcgcgg tggtgggtgc gttcatcatc ggcatttgtg
      121 agaacctcat caagggactg gacgtgatcc tcatcaaatc cggcgtgacc aagcagatgc
      181 tgggactgac cactttctcg gatgcgttta ccttcgcgct gctgattatc attctcatcg
      241 tcaaacccac gggtctgttc ggtgaaaaaa cgacggataa ggtgtgaggt gagaaaaatg
      301 accattcccg aaaaagctcc ccggatgcaa aataccaagc ccagtctggg cgcggtgatg
      361 accggcgtga tcgtgcttct gatctatgcg gcggtcttcg cgctggaaca gatcatcccg
      421 cccacctcca tgcttttcac cgtgctgaaa aagggcgcca cctacgcgct ggtagccgtt
      481 tccatgaacc tgctcaacgg cttcacgggt ctgttctcgc tgggacaggc aggctttatg
      541 ctcatcgggg cgtacaccta cgccattctg accattccgg tagaaagccg ggccgtggtg
      601 tatcagtact atgacggcgg cctgatccag ttcgccatgc ccgtcattcc cgcgctgatt
      661 ctggccgggc tgatggcggc ggccttcgcg ttcctgatcg gcctgccggt gctgcggctc
      721 aagagcgact atctggccat tgccacgctg ggctttgcgg agatcatccg ctccatcttc
      781 cagtgggaca aactgggggc cgtcaccaac ggttccaata tgctccgcct tttccccacc
      841 tttaacgatt tcaacatccg aaacgcggaa ggagaagtca cgctctactt ttccaccacc
      901 attccattcc tgatcgcggg cgtatgcatc ggcctgatcg tactgctgct ccattccagc
      961 tacggccgcg ccttcatgtc catccgggac gatgagatcg ccgccgaggc catgggcgtg
     1021 aatctggccc ggcacaagca gcaggccttc tgcatcagtt ccttctttgc gggcgtaggc
     1081 ggcgcgatgc tggccatgta tcagaactcc gtgcaggcca agagcttcac ctcggccatg
     1141 acctacgaaa ttttgctgat cgtggtcatc ggcggtatcg gttctgtcac cggcagctgc
     1201 ctgagttcgt tcctgttcgt tgcctgctcc gaatggtggc tgcgctttct ggatcagaag
     1261 cagatgatcg gcacatggga agttcccctg ctccgcaacg gcttccgtct ggtggtcttc
     1321 tccatcatca tcatgatcgt ggtgctgttc ttccggcagg gcatcatggg cacccgtgaa
     1381 ctcccagatc tcttccgcaa aaaaacgcag aaagggggcg cggtgaaatg agcaatggcg
     1441 tcaagaccat tctggagctg gaaaacgtga ccatgcagtt cggcggcgta gtcgccgtgg
     1501 acagccnnnn nnnnnnacgt ggacaagggc gagatcgtgg ccctcatcgg ccccaacggc
     1561 gcgggcaaga ccacggcctt caacgtggtc accggcgtat acgcgcccac caacggcaat
     1621 gtgaaaatgc ggggcaagat catcgcgcag ggtcatccca caggcaaaat gaagaagctc
     1681 tacgccggaa gccacatcgg cgaatacggc aagctcatct ccaacacgcc ggacaagatt
     1741 accgaaatgg gcgtggcccg cacctttcag aatatccgtc tgttcaaggg catgacggtt
     1801 ctggaaaacg tactggtagc ccgtcacatg cgcatgaagg cggggctttt ctcggcggcc
     1861 ttccacctga acatgcagga agaaaagcgc atccgtcagg aatcgctgga tctgctctac
     1921 ctgctgggcc ttggggacgt agcggacgaa aaggcttcgt ccctgcccta cggcaagcag
     1981 cgctatctgg agatcgcccg ggcactggcc acagaacccc ggctgttgct gctggacgag
     2041 cccgccgccg gcatgaaccc gcaggagacg gacgaattaa gcgaatttat cctcaagatc
     2101 aaggaagatt tcaagctgac gatcttcctc atcgagcacc acatgaatct ggtcatgggg
     2161 atcagcgatc gaatctacgt gctggacttc ggccgactga tcgccgaggg cacgcccgag
     2221 gatattcagg cgaatcccga cgtgatcgcg gcctatttgg gggtggagga agaatgagta
     2281 tgctgg
//
LOCUS       DAWAEA010000220         2282 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6610_length_2282_cov_2.476426, whole genome shotgun sequence.
ACCESSION   DAWAEA010000220 DAWAEA010000000
VERSION     DAWAEA010000220.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6610_length_2282_cov_2.476426"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    267..411
                     /estimated_length=145
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          344 a          710 c          652 g          431 t
ORIGIN      
        1 gcagaggggc tcggcctgcg ccgctgaaaa ttccgccacc agatgattct ccgtgttttc
       61 ttccacgaga gctgacgcgc ccagccccgt gtaaggcccg ccccacgtga tccgcacggc
      121 cgggacagct tccacctccg gcacgatgat gggtacgtcg atggtcaccg tgtctccgtc
      181 ccgcagattt ttgccgtctc cgtgccggat cacatactcc cccacccagc gttcaggcag
      241 cgtttcgcgc agttctcgaa tggaaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncaagcggct
      421 tttccacatt gagcagatat tcctcatggg tcttggcgcc ccagctgtcg tcgccgccca
      481 cgcccatctg ctgcttggcc acgcgcacca ccgtgttgta gaccggcggc aattcgttgg
      541 gatgacgggc gttttccagc tcgtgggggg tatagggcag cacggaggtc tccatgccgt
      601 tttctgccgc gcacaggaac cgcaggcccc ggccgcgata gtccgtcact tccgcccagc
      661 gcacgccggt gcggttgccg cattcctgag gcagcacata gcgggagacg ttctcctgcg
      721 ccgtggtctc ccacaggccc agcttcgcgc catggcagcg atccacgtag ttttcgcccg
      781 gccccatgcc gtaatagcgc acctgatcgt actgcgcgtc cagcatgaac agcatcccga
      841 actccggcat gtcgcccagt tccttcaccg ggtcgtagcg cagctccgcg tccacccggc
      901 cgcaggggtg cacggtgtag cgcaccgcgc actgggccgc cggggtggtg ggcagcgcgt
      961 agagataggt gatgctcacg ccgccgtcca cctcgttctg cacctgcggc ttgtccatgg
     1021 gcagacagta cagggacgct agcttccact ggccgtagcg ctgaggcatc cgattgccgt
     1081 agtcgttttc cgtgggggcg cgccagaagt tgggcatggg cgcggctttc agcatctcca
     1141 cgccgcctgc gcggtaagag atcatcttgc cctgcgtttt gctgaacagc acgctgaaat
     1201 gctcgccggt cacgcccaga ttgttgaagc cgtcgaccac gcgaaccggc gcggcgggaa
     1261 cggcggtctt cttttcctct acccgccacg tgccctgacc gaacgccact tcatagcccg
     1321 ccgcggccca ctcggtggcc tcccgctgac ggaaggacag ggtcacggtg tactcgccgc
     1381 cccgggtctg ttccgggaag ggcaggggca gggtggccgt ctcgccgggg gccacgtcgg
     1441 tcttgagctc ccgacgggcg atctccctgc catcccggct cagcgtggcg acgcaccgcc
     1501 acgcggcggt gctggtgaac aggctgtgat tgcggatggt gacgctgtcc cggctcacat
     1561 cggcaaacac actctggtag ttgtacttga cctcctgcat cttgggggac tcgctgccat
     1621 cgccgaacac gatgccgttg cagctgaaat cccagtcggc ggggcgttcg ccaaaatcgc
     1681 cgccgtagaa atacgcgtcc ttgccgtaac gatccttcac gcggatgctc tggtcgatga
     1741 agtcccagat gaagccgccc tgatacaggg gctcttcata ggcgtactcg gtatatttgt
     1801 gcatcgcgcc gttggagtta cccatggcgt gggtgtattc gcacaggatg aagggcttgt
     1861 cccggtgctc ctccagatac tgacgaatct tccagacggg ggtgtacatc tggctttcca
     1921 tatcggtggt gtcgttgtag cgccggtcgt gggccgtgcc ctcgtaatgc accagccggg
     1981 tatcgtccag ctcccggaag cgctggctca tggcgtaggg cacgctgccg cccagcgatt
     2041 cgttgccgca gctccagatc aaaatggaag gatgattctt gtcccgctga taggtggact
     2101 cgatccggtc cagcatttgc gccttccagt cctcccggtc tccgggaacg gcaaagctca
     2161 gcggcttctt cccagcgccg atctgctccc acacgccgtg ggattccaga ttgctttccg
     2221 caatgacgta caggcccagc tcgtcgcaca gggcgtacac cgcgtcatgg tcggggtaac
     2281 gg
//
LOCUS       DAWAEA010000221         2277 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6630_length_2277_cov_1.252475, whole genome shotgun sequence.
ACCESSION   DAWAEA010000221 DAWAEA010000000
VERSION     DAWAEA010000221.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6630_length_2277_cov_1.252475"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    296..473
                     /estimated_length=178
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          451 a          603 c          637 g          408 t
ORIGIN      
        1 ggatcatgcc gtccagcgag aagcggaaca cattgtcata ctcccgcacc acggcgaccc
       61 ggcctgtgag gtcggtcaga ttgtacacca ccgaccactg ggtcatgctg gtggcttcct
      121 cggtctcggg ctgaccgatg acggccagca gggccatgac ctcggcttcg ctcatcacgc
      181 cttttttgaa ggcgagggtc tgctcgatca ggtcgtagcg atccttgccg cgcaccggcg
      241 cgtagggcac gttttccgtc aggtagaaat tggtcacgta attctgatcg tacacnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcatatc
      481 atactgatcc agcagctcca gtgcttcgtc cacggtggcg gcgcgatcca gcaccagccg
      541 cagggccacg gtggtggaaa ttttgttctt gcccgtgtcc tgcagcgtgg ggtcgccccg
      601 gagcttcagt acgctcattg ccagcccctt ctcgttcatg ccgtccatca gcaggtacgg
      661 gaagaacgcc gccatggata gatcggtggt tccgtcgttc agggtaccgg cggtgtagcc
      721 gatccagccc acgttggcaa gaccgatgga acgatagccg ttttccggcg cggtgcggac
      781 gagaacagcg gtcatatcca ttttataatc gaaattgcgg ccgtagagaa actttccctc
      841 ctcgctctgc gcggtaaagg cgctgcatcc cgcgtccacc gcgcccaccg aggcggttcc
      901 cccggccagc agggtcttct gcaaaaagcg caccatgccc tccacgtcgg tcacattggc
      961 ttccagcagc gcgtccagct ggtaatcggc ggtataatcc atggtataga gcttaccgtc
     1021 gttcacatcg gtcagggtgg ccacagagac gcgctgttcg tcggttttca gccacgcggc
     1081 cgggtttgcg ctctcggcca tggccggggc agtgctcagg agcatcagga caatcaggaa
     1141 cagcgcaagg cattttctct tcatcaattt tgttcctcct tctttttcat ggcaggacgt
     1201 acatcggata gacggatcat ttcgttgaat gattgaaata taccagaatc ctcccaaaaa
     1261 tgcaatgatg aaatcggggt tccatatata tcccttgcat ttcaaggaat gtgccggtat
     1321 aatcatggga cggcggcctg aaaccgccgc gagcgaattt tgcttgaaac acacgcctgc
     1381 gctgggcgca ggcggatgga ggcttggtat atggaaacga cgcgcagggc gagaaccgtc
     1441 tgtatgccgg tgctgcccct tcggggaatg atggtattcc cccatatggt gctgcatttt
     1501 gacgtaggca gacccaagag cgtagccgcg ctggaaaaag cgatgaccgg cgaccagaaa
     1561 atttttctgg tggcccagaa ggatgcggag aacgacgacc cgacccggga cgatctttgc
     1621 cgggtaggca ccattgctgt ggtcaagcag gcgctgaacc tgccgggcga cagcatccgg
     1681 gtgctggtgg agggcctgca gcgggccgtt ctgctggagg tgtccgagga agaaccctgc
     1741 atgatggcgg acgtccgccc ctcggtggac accagcctga aggacagccc cgaaatgaag
     1801 gcgctggtgc gaaccacgca ggaatacttc gaacagtact gccagaccat tctggagcag
     1861 ctcaaggccc gggacctgct ggagacactc tgcggcatcc tgctgcggga gacggagctg
     1921 gctgacgtgg aaaagcaggt gcaggcccgc atcaagaagc agatcgagcg caaccagaag
     1981 gattattacc ttcgggagca gatcaaggcc attcagactg agctgggcga caaggacggc
     2041 accgatgtgg aggagcttcg cgagaagctg gaaaaaacgc ccctgaacga tgaagcccgg
     2101 caaaaggctg aaaaagagct ggaacgcctg tcccacatgg ctcccggcac gccggagatc
     2161 ggcatcagcc gcacctacgt ggagtggatc ctcgacctgc cctggggcaa gaccaccacg
     2221 gacaatctgg atctgaagcg ggcccggcgc attctggatc aggatcacta cggactg
//
LOCUS       DAWAEA010000222         2269 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6655_length_2269_cov_1.538844, whole genome shotgun sequence.
ACCESSION   DAWAEA010000222 DAWAEA010000000
VERSION     DAWAEA010000222.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6655_length_2269_cov_1.538844"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    337..471
                     /estimated_length=135
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    776..785
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1213..1298
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          347 a          585 c          702 g          404 t
ORIGIN      
        1 atggggaaaa cgccgtctgc gcctacaatc tggcccagcg gttcgtggtg gagcattccc
       61 ggttcggcat tcccatgctg gtcagcagcg aatgtcccca cggccatcag gcgctggacg
      121 gctatctgct gccggtgaat ctggcggcag gggctacctt tcagccggag cttctttatg
      181 aggcaggaaa ggtctgcggg cggcagcttc gggagctggg cgtggacttg gccctggtgt
      241 ccatgctgga tatcctgcgg gatccccgct ggggccgcag tgaggaatgc tacggcgaag
      301 atcctttcca tgcggcgcag tttgcccgtg ccatcgnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctgcagaaa
      481 atcctccgcg gggagttcgg cttcgatggg atcgttatgg ccgacggctg cgccattgac
      541 cggctgaacg accagaccgg cagtacagtg gcttccgggg cgctggccct gcgctccggg
      601 atcgacatca gcctgtggga cgagggcttt tcccggctgg aggaagcact ggaaagtggc
      661 ctgatcgacg aaaaagttct gaatcgggcc gtggagcggg tgctgacgct gaaattggaa
      721 cggggcctgt ttgagcatcc ctacctcccg gaagaacgaa aaccttccgt cttttnnnnn
      781 nnnnnaacgg cagcgctgat cggcccggcg gcggacgatc tgtaccgtca gctgggcgat
      841 tacacgccgc cagtggagtc ggacgcgggc tgtacgctgc tgcagggtct ccgggagctg
      901 gccgacccgg aaatgcgtct ttggtacaac gacggcagcg accccaagcg ggctgcggcg
      961 ttggcggcga aggcggatac ggtggtgctg gcgctgggcg gctcgtccag ccggttcgaa
     1021 caggcccgtt ttgacaaaaa cggcgcggcg ctgaacggca gcatggactg cggcgagggc
     1081 gtggacagct cggcactgcg gctcccggac ggacagcatt cactgttcgc taaggtgcgg
     1141 gccgccgcga aaacgctggt cacggtgttg atcgttggac ggccctatgc cgtgccggag
     1201 atcgccgaag ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct tttatcccgg cccgtggggc
     1321 gggcaggcat tggcggaagc cctgctgggg cggttcagtc cgtcgggacg gctgcccgcg
     1381 tcctttcccc agcatccggg ccagctgccg gtgtactata accccaaggg gggcacggtt
     1441 ccgtggaact atgccgacct aaaggatggg cctctgttct ccttcggcga tggcatgggg
     1501 tatgggcata ttttgtaccg tgattttgag ctgctgccga agctgctgcc ggagggacgg
     1561 ctccctgcgg acgatacgcc ggtgctggaa ctgaggttcc gggcggaaaa tcccggcccg
     1621 gaggaggaac acgccgtgcc gctgcttttc gtcaccgatc tgggatcgga cgtggcgcgc
     1681 cgggtgcggg agctgaaggt ctttgccaag aaagctgtcc cggcggggga gaacacggca
     1741 ttttccctga cgctgacggc ggacgatctg gccgtgtgga accgccgcat ggaacgggaa
     1801 ttgcagcgcg gccgtttccg gctgcggctg gaggacggcg gccgcctcct ttatgaaacc
     1861 gtttgggagc tgaaatgaat caaaaaatcc ccgccgcagt ttcgggaggc caaagcgcgg
     1921 cggggattct gttacgtttt cacttagttt ccgcactcca cggaaatttc attgaagagc
     1981 ttgagcacct cgtccagctg ggtgtcctgc ttttcctgcc cggcgcggtc cagcacgacc
     2041 ttgccctcgt ggagcataag cagccggtcg ccgtattcca gcgcgtagcg caggttatga
     2101 gtcaccatca gggtggtcag cttcttttcc ttcacgattc ggtcagtcag gcgcatgatg
     2161 atgtccgccg ttttgggatc cagcgcggca gtatgctcat ccagaatcag aaattcgatg
     2221 ggggtcatgg tgctcatcag cagcgccatg gcctgacgct ggccgccgg
//
LOCUS       DAWAEA010000223         2268 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6659_length_2268_cov_1.802531, whole genome shotgun sequence.
ACCESSION   DAWAEA010000223 DAWAEA010000000
VERSION     DAWAEA010000223.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6659_length_2268_cov_1.802531"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    211..220
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          517 a          687 c          597 g          457 t
ORIGIN      
        1 gtgcctggaa gagtgcttcg acgctttcgt ggagcccaat ctgatccagc ctaccttcat
       61 caccgactat cccgtggcga tctctccgct ggccaagcgc aagaaggacg agccggagat
      121 gaccgagcgc ttcgagttct tcatcaatgg ctttgagttc ggcaacgcct attccgagct
      181 gaacgacccc atcgaccagc gcggccgctt nnnnnnnnnn gccaactccc gctccttcgt
      241 cacccaccac aacgcgctgg acattcccat gtacctgcgt attgagaccg agctgtacct
      301 gaagcggctc attgtgggcg gcttcgacaa ggtgtacgag gtgggccgta tcttccgcaa
      361 cgagggcatg gacacccgtc acaatcccga atttaccacc atcgagcttt atcaggccta
      421 tgccgactat caccagatca tggatctggt ggaggagctg tacatctacg tcaccaaaaa
      481 ggtctgcggc acgctgaaga tcccctatct gggcaaggag atcaacatgg aaggcccgtg
      541 ggcccgcatg accatggccg aggccgtgaa aaaatactcc ggcatcgact actacgcctg
      601 ggaaagcgac gagcaggccc gggcggactg cgaagcccac gacgtgcatg tggaaaagga
      661 cgcggtgaag ggccagtgcc tggaagagtg ccatgaaacc catcggcacg cccgctccct
      721 ccgccaaggc ggcggaagaa aaggctcccg agacggcggc tcccgctgcg gaaaccgtgg
      781 ccgagcctat cgacttctcc aaggtggaga tcgagcctct gttcaaggat tatgtggact
      841 ttgagacctt ctccaagagc gacttccgcg ccgtgaaggt caaggcctgc gaagcggtga
      901 agaagtccaa gaagctgctt cagtttacgc tggacgacgg caccggcacc gaccgcacca
      961 ttctcagcgg cattcatgcc tattacgaac cggaggagct ggtgggcaag acctgcatcg
     1021 ccatcgtcaa cctgccgccc cgtcccatga tgggcattta gcctggaaaa tgctattgcc
     1081 cttattcccc tgaaaaaacg tatatgcctt tgcttttcaa ctctttatcg aaatataaaa
     1141 agaccgctct tccagctgta tggaaaagcg gtgtcttttt atcacaattt cgaaccccat
     1201 caggcttcca cgttggattt cgtgggcagc gcacgctgca cattaccgct gcgcaggcaa
     1261 cgggtacaca cgttcatccg cttcggctga ccattcacca gaacgcgcac attctgcacg
     1321 ttgggagccc aaacgcgatt gctcttgcgg ttcgagtggc tgacgttgtg gccgctcatc
     1381 gcgccctttt cgcacacttc gcaaaacttg cccatcatta cacctcctaa cgggatgttt
     1441 ggattcataa cagcttgact attttagcac gctttttccc atttggcaag gaaaatttcc
     1501 gttttcccga cattttccct gaaaatgttg tgtgaagccg tctccgggtg atacaataaa
     1561 cccggaagag aaagaaaagg agaacgctca tggcctttgc ccatctgcat ctgcataccg
     1621 aatacagcct gctggacggc gccaaccgca tcggcccgct ggtcagccgc ctgaaagaac
     1681 tgggctttac ccacgccgcc atcaccgacc acggcgtgat gtacggcgcg gtggattttt
     1741 ataacgcctg ccgggacgcc ggcatcaccc cggtcatcgg ctgcgaggtc tatatctgcc
     1801 gcaatcgttt cgaaaaaagc ggcgcggccc gggaatacag ccatatgatc cttctgtgcg
     1861 aaaacaacac gggctatcag aacctcatca agctggtcag tgccggcttc accgagggct
     1921 tctattataa gccccgcatt gactacgacc tgctgaagca gcacaccgag ggcctgatct
     1981 gcctgtccgc ctgcctcagc ggcgacctgc ccaaaatgct gctggagggc cgctacgacg
     2041 acgcgaagac ttacgccctg caaatgcagg agcttttcgg cagggatcac tactacatcg
     2101 agctaatgga tcacggcatc cccgaggaaa agaccgtgct gccccggctg gtgcgcgtcg
     2161 cgcaggaaac aggcatcccc atggtggtca ccaacgactg tcactatctg aaccgagaag
     2221 attcaaaggc gcaggaggtg cttctgtgca tccagaccgg caagaccc
//
LOCUS       DAWAEA010000224         2260 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6678_length_2260_cov_3.331973, whole genome shotgun sequence.
ACCESSION   DAWAEA010000224 DAWAEA010000000
VERSION     DAWAEA010000224.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6678_length_2260_cov_3.331973"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          527 a          692 c          627 g          414 t
ORIGIN      
        1 ttgtttgcct gaactccggg tcgctaaccg ccttcggcgg ttgtatccac tggatgacgg
       61 tgaacttttc aaatttaatt tgaaaagttc agtctgccaa aggcagaccg ggttgcccaa
      121 gggcaacccg ccttgcgtct tcccttcgtt cccctttgga aaccttcggg caccaaagtt
      181 ggcaagaaaa cgaagggact gtaaccatcg ctctcgcgga caaatattcg ctttttgaaa
      241 agaacaaagg ggggctttcc tcgaagcgcc cctcactgcc gtgagctccg ctgtcagcgc
      301 ttcaactgcc acgcagtgca gaacccctcg ccgaccggct gcgcaccagc cctcaaaccc
      361 cgtttttctc cttcaccccg tccccgaacc tgtccagcgc cagcgccgcc gcaaacccgg
      421 ccagcgcaca ggccgcgcag gcgatcccct gcgatacgct gtcataatgc aggggcacga
      481 tcatcagcgt agacgcgcat accacgccca gaatcacgtg ataaaccagt gcatagtggg
      541 tccccagcaa gtggttcaca aaccgggcgg tcagaagcgc cgtcaacaga atgccgcaca
      601 gcagcggcag aatgcatccc cactggatct gcacaagcga aggaaaggcc ccgccctttc
      661 ccatccagaa ggcgaaaaca tccttcaccc acgtggcgat ggaaccgatg cccgccgtca
      721 taggctcgta cagccccatg aaaatcagga tggacgagga cgaaagtccc ggcaaaacca
      781 gactcagccc ccataccgcc ccgcaaaaga aataccatcc gctgtttggc tgaatggccg
      841 cagcagaaga aaacttgaac aggctcaaca accccagcag gatcacaaag ctcaacccga
      901 acgcccagcc gcaacggacg ttccgtccct tcagtcccgc ctcccgccag agagaaggga
      961 tcgtacccag gatcaacccg aagaacagac aggtcacgat ggccgaatct gccccgaagg
     1021 ccaactccac caccttcgac agccctacaa acccaatggc gcagccgatc agtacgggaa
     1081 taaacagccg gtagtactgc cggaacgcct tgcccggatg ggatagaagc gccatcatgg
     1141 gctgatagat gccgaacagg acgcacatca cgccgccgct gacgccgggc aagattgcgc
     1201 ccacgccgat cagcgcgcca tgcagcacgt gcatcagcca ttgaccggcg atcattttcg
     1261 ttgtttcgct ggaatgagcc attctttttt tcctccttca ttggcaaact gcccggtcat
     1321 catagcatac ccgcggcagt tttcccataa gtctgtggaa aaacagacag gttcttaacc
     1381 tgtttcaaaa ttttatgaga ataatgccgc tttttcttta acggcccgtt tatttacggt
     1441 ttcttcacag gtttgcagtg agttcttcat gcgggtcgtg tatgatagac tgggaaagcg
     1501 taccggcgcg ccgccttttt tgcggctgcc ggaattatgg aaggaaagag tggaacgcat
     1561 ggaaaacgca aagaaaaaga gcagaaagaa aaaagtgctt atttgggttc tgatcgtcct
     1621 gctggcgctg gccgggggcg ggtggtatgc catgaagcgg atgaacgacg cgatgcagac
     1681 tgccgccagt gctcagaata cctacaccgt ggcccgcggc gacgtgtccg tcaccatcac
     1741 cggcagcggc aaattggaaa cgcccgatac ggcggatgtg accctgcccg tgggcgtgaa
     1801 ggtggatacc ctgtttgtga aggagggcga ttcggtcaag gcgggcgacg tgctggccgc
     1861 gctggacggc gattccctgc agtaccgggc ggcggagctg agctccgaac tggctgcgct
     1921 ggatcagcag ctgggctccc gcaagacccg cagccgcatc accgccccgg tggaaggccg
     1981 catcaagtac cagcccgcag ccgcgggaga cgatgtgatc gaagccgtca acgagtacgg
     2041 ctgtttggcg atcctttcca ccgacggcag tatggagttg cgccttcaga ccgacgcggc
     2101 tctgtccctg aacgacaagg tcaccgtcca gtgggacggc ggctccgccg agggcacgct
     2161 ggtcagttgg ttggacggcg gctggctggt aacgttcagc gacgaaaagg ctccctatca
     2221 ggaaacggcg gaagtatatt cggactccat gctgctgggc
//
LOCUS       DAWAEA010000225         2259 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6680_length_2259_cov_3.540381, whole genome shotgun sequence.
ACCESSION   DAWAEA010000225 DAWAEA010000000
VERSION     DAWAEA010000225.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6680_length_2259_cov_3.540381"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          353 a          771 c          700 g          435 t
ORIGIN      
        1 cctcccgccg gggcgtcacc gtggcggact atgcccagac cggcagcctg gaaacgctgc
       61 gggccatcat ggatttccat ggcggtctcc cggatgccaa aacggcgggc agtatccttc
      121 agctgcgcta ttatctggag ggccccgcca tggaggagct ggctgcgcgg cacacaccgg
      181 cagacatggc agcgctgcag gccttacagc gccaggcgga gcaggcggcg caggagggga
      241 cggatgcgct ggccgaggcc ctgttccgct accaccgggg catcaccttt ctcagcggca
      301 acaccatcac accgctgctg ttcaacgcct ttacccgggt gaacctggag ttctggaagg
      361 agtatatcca gcttaccggc atcgaccgct gtctggaacg gctggcccaa ttcaccgccc
      421 atatcgaggc gggggaggga caggccgcct cgtccctgct gcggcagggc ctggagcagt
      481 tcatcaccca ggtgcggtag cttccgtcag ctcttcggag taaacacccc cggtcgaagg
      541 ccccgcagcg caaaaacacc cggcctgacg gccgggtgtt tttactatgc ttaccgacgc
      601 ttgccgccgc cgaaaccgcc gccgccaaag cctccgcctc gggagcttcc tccgccgcgg
      661 gaactgccgc cgaagccgcc gccgctgaac ccgctgccgc cgctgggacg actgggccgg
      721 ctgggccggg gcgcactggg tctcggaccg ccgccgggcc ggttattggg ccggggcggc
      781 ctcggtgcgc cgggcctcgg gccgccgccc atgggaggcc ggggaccacc gctgtagggc
      841 ggccggggcc cgccgccgtg aggcggccgc gggggtcggg gtgctctggg aggccggggc
      901 ggcgcgtacc gtctgcccca gaaaatggga tagtacaccg gacgcaccac gtttaccggc
      961 gcgcccacga tataccgccg gcgatagcgg ctgtaccgca gagcatccag caccatccac
     1021 accacgaaga taccgatgat cagcaccacc acgctgccga tggacgtgcc gccgctgctg
     1081 gccgccggtg ccgccggggc gtaccagccg ctgtcattgc tgtcgccgcc gtccagcttc
     1141 gcctgggcgt agaacacgtc cgcctgccgg aagaaggctg tcacggcact gtcgaagtcg
     1201 ccgctgtaat aggtggtctc aatggcctgc cgcagctgat tttgctgggt gtcggtcatg
     1261 gtgtagccca cattgtcgcc gcagtagacc tgccagtcgc cgtccttcac cagcagcagt
     1321 atcatgtcgt tgcgtcccag cccccacttc tcgcccaggt tggcggcata gtcctccgcg
     1381 tcccagccgg tcagatggtc gatgctggcc accgccgtaa tggcgtaata cttgctgttc
     1441 cagctgtcgt tgtagtcccg gatcagctgc tccgtctgtc cgttcagcag accggcctcg
     1501 tcacagatcc agtcctggta ctgcacgtcc agcagctccg tgggcttctt ggcgataaag
     1561 ccgtccttgc ggctctggcc gatgaacacc gcgccggcaa tggccagcat cagcaccacc
     1621 agtgccacgc cgcgtttttc aaaaaacttc atacctgttc ccctttccga aggagattga
     1681 tcttattttc agctccgcag ctccatgagc ttctgcttca gctcaccctc gatcttaccc
     1741 agcgccccat tttgcgctgc gcgcaaaacg gggaccccgt atgatctgac atcctcggac
     1801 gaaatgaatt cgtcctgcgg caaggcttcg gcaagccgaa acgcttgtgc gcgccgtccg
     1861 gcgcgtgtgc ggctttgccg cgcagtcgag ctgggctgac ggtttgtgtg tttcgatcag
     1921 ctccgcagct ccatcagctt ctgcttcagc tcaccctcga tcttacccag ctcgacttcg
     1981 gcctccttgc gcttggccgc gccctccttc tggatgttca gcacctcgtc cagggtggag
     2041 atcagctgct ggttggtgtg ctgcagcgtc tcgatatcga tgatggagcg ctcggcctcc
     2101 ttggcggtgg cgatggtgcc catcttcagc gtatcggcgt tcttcttcag cagatcgttg
     2161 gtcatctcgg tgacggcatt ctgtgcggcg gtagcctgac ggccgtgctc catgcccagg
     2221 gccagcacca tctggctctt ccacaggggg atggtgttc
//
LOCUS       DAWAEA010000226         2257 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6686_length_2257_cov_2.198456, whole genome shotgun sequence.
ACCESSION   DAWAEA010000226 DAWAEA010000000
VERSION     DAWAEA010000226.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6686_length_2257_cov_2.198456"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          435 a          705 c          672 g          445 t
ORIGIN      
        1 tcgctcattt ggtttcctcc ttcaggctca gtacggaacg gttcttccag aaatagtcaa
       61 tgccggacca cagggtcatg gccacggaag cccagaccat cagcttgccc accgtgaagc
      121 cgccggcgct gtaggcccgg ccgtccagca ggaaaacgat cagggtcaac agctgggtgc
      181 aggtcttgat cttgcccaac tttcctgcgg cgatcacgct gccctgctgg gaagccacca
      241 gccgcagccc gtccacggcc agctcccgga acagcaccac aatacaggcc caggccggaa
      301 actcgcccag accgcacagg gcgatcatgg tgctcaaaac cagcagcttg tccgccaccg
      361 ggtcaatgaa tttaccgaag gtggtgacga tatgctgccg ccgggcaatt tgcccatcca
      421 gaaaatcggt aaaggcggcg atgcagaacg cgatcagcgc tgcggtgcgg gcggtctgtc
      481 cgggcaggta cagcagcagc acgcacaccg gcaccagaca gatgcgcagg atcgacagac
      541 ggttgggaat attgaggttc ttcatggacg tcaatcctct ctgatgcgtt cggcacgcag
      601 gtcgtagacc tccgcgtcgg tgatacgggc gcgaacgaac tgccccagat cggcctcgga
      661 aacgccggtg agcttgatca cgccgtcggt gtccggcgct tcctgcgcgc tgcggcctac
      721 gccgtggcct ttgccgtcca cggtctccac cagcaccttt tcttccgtgc cgatccgcag
      781 ccggttccgt tccagcgaaa tcgcctgctg agcggtcatc agctcatcca gccgcttctg
      841 cttgatctct tcggggagct ggccgtccat ccgggcggcg ggggtgtcct cttccggcga
      901 ataggtgaaa gcacccagcc ggtcgaactg aatttcccgc acaaagccca tcagataggc
      961 gaactgctca tccgtctcgc cggggaagcc cacgataaag gtagtgcgca gggtgaagcc
     1021 catttcccgg gcccggtgca gcaactgccg ggtcttttcc gggtcgccac ggcggttcat
     1081 gcgcttcagc agcagcgggt cggcgtgctg caggggcaag tccagatatt tgcagatgtt
     1141 gtcatggcgc gccatagcgt tcagcagttc ttcgtcgatc tcatccgggt agcagtagag
     1201 cacccgcagc cattccacgc cgggaatttc cgacgcccgt tccagcagct ccggcagctg
     1261 ggaatggccg acccggtcga tgccgaagcg ggaggtgtcc tgcgccacca gcacgtgctc
     1321 cttcacgcct ttttccgcca gcgtgcgaat ttcatccagc acgtcttcca tctcccggga
     1381 gcggaagccg ccccggatca ggggaatggc gcagtaggcg cagcggttgt cgcagccgtc
     1441 agcgatacgg atgtaggcgg aatagggcgc ggtggtcagc acccggctgc cggacaggat
     1501 ggtattgtcc cggccgcaga agctctggcg cttgccgtgc agggccgcct ccagcgcctc
     1561 cggcagacgg gggtactggc tcacgcccat cagcagatcg atctccggca tatcagtcat
     1621 gagctctttt tcatagcgct gggccagaca gcccgtggcc accagcacct tgcacttgcc
     1681 ggtgcgcttg tattcggcca tctgcagcag agtgtcgatg ctctcctgct tggcggggtc
     1741 gatgaagccg caggtgttga cgatcagcac ctcggcctcc ttttcatccg cggtaatggt
     1801 atagccgtgc tccttcagga tggaaagcat cagctccgta tccacccggt ttttgctgca
     1861 gccaagggaa atcacgccga ctgtgggcac tcaggtcgcc tccgaaatta aaaatcaaaa
     1921 acgggataac aaaaggcgaa agaacccaaa aatgttcttt cgcccttcat tttatcatac
     1981 ttttccggga ttgaaaaccc ttttcccgtt cggtgctaca gggcgttttc gcccttggaa
     2041 ggcaacagag aaagctcctc cagaatccag tccagctttg cctgccattt caggaaatcc
     2101 atgcagcgga tgtagggggc ttccagccgg tcatggaggg ctttggcggc ggaaagctgc
     2161 tcaacggtcc gctggcagag cagttcatag gcgttctggt cgaagcgttc ttcctcccgg
     2221 ggcttcaggg caaagagccg ttccggcggg agcgcgt
//
LOCUS       DAWAEA010000227         2245 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6724_length_2245_cov_1.568950, whole genome shotgun sequence.
ACCESSION   DAWAEA010000227 DAWAEA010000000
VERSION     DAWAEA010000227.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6724_length_2245_cov_1.568950"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1495..1504
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          423 a          613 c          703 g          496 t
ORIGIN      
        1 catccgcacc attaaggccg tggttcagac cgccgttgct gccatcagca ccgccgccgt
       61 gctggcggaa gtgggctggc tcatggacgt ggacggtcag tacggctttg tgaaagcggc
      121 gaacgtcagg acggccgcca gctttgttca gctggattgg cggatggaat catcagatca
      181 ataggaacaa acgccccggt cagttcgccc ggggcgttct tgattcccag cgccgatttc
      241 gctataatgg gatgaaacca tcggagaaag gcggacggag agaatgacag gcaaggggct
      301 ctttcggaga atgctggggc tttgcggcat cctgctggcg gggatcgccg taggctttgc
      361 gctgctgacg gcagtgtttt tgctgccggt ggacgcgatg gaggagcatg tgctggcttc
      421 gatccccgct ttgagcggcg aatggggcac gggggaggag tcctacgagc aggtgctcaa
      481 gggatacgcc tccacccagc tggataattc caccgatgct tatatgctgc tgagcgccat
      541 tcatcgttca gataaaagcg cagtggatca ggccgtgaat gtctatacat ggcaggatcc
      601 agacagcttc ggccagtacg gcacactgct gcgctatgga aaaagcggct cggagggaat
      661 gcaggaaaac gccaccgccc gttactggct gggctatctg gtggtgctca agcccctgct
      721 tctgttcctg aactatatgg atattcggat gctgaacatg atcgtgcagc tggggctggt
      781 catgctgctg tgctgcctga tgcagaaacg ggggctgggc cggtatgtgc tgcccttcgg
      841 cctgtcccta ctgtgcatta cgcccggcgt gacgtggctg tcgctgcaat tttctaccac
      901 gctgctggtg gcgcttgccg ccatggccgt gctgctctgg aaaccgcagc ggatgaaaac
      961 gccgatggca gaggatgtgt tcttcctgct gacgggaatg gcgaccagct actttgattt
     1021 tctgacctat cccattgcgt cgctgggaat gccgctgatc gtgtggctgc tgctgcaccg
     1081 ggacgagacg gcacaagccc gcctcgggcg gatggtgcgc tgcggtctgt gctgggcatt
     1141 gggctatgcg ggcatgtggg ccggaaaatg ggcgctggcg cttttgtacg gcagcgaggg
     1201 cttttggtct acgctggtgg gcagtctgga agtgcgcacg tcccacgagg tgcggggcgt
     1261 gggggagatc agccggttgg acgctctgtg gaaggccgcg tccgtgtttt tcaagaagcc
     1321 ctacttcatg gccggggcgg cggcgcttct cggctatggg gccgtatttg cccgccgcag
     1381 ggtcaaggga tttccactgg aaaaaaacag ccgcggactg gccgccgcgc tgggttgcgt
     1441 ggcgctgctg ccgctggggt ggtatctttt taccgccaat catacctaca atcannnnnn
     1501 nnnncgccct gtgcgtgacg gttttcggcc tgacgggcgc actgacgtgc cgcctcccgg
     1561 caagaaaaaa gaaacaaaat tgaattacgt tcaaatttca tttggaaaat cggagttctc
     1621 ctattgctaa ctccgatttt ttcttgtatg ataggcgagc gagacaaccc aacgccgcac
     1681 gccggccgtc ccgggatgat cccccgaagc ggctgcgcat ttcaaatatg tcccatatcg
     1741 ttgcttcctg attcgacaag accaaaggag aatatttgcc atgcctcaaa caaagacaca
     1801 ggccggaggg aagaaagccc ggttgctgtg gtacttcctc aggggcagca agcggtttta
     1861 tctggccgct gtgctgtgtt ctgtgtgcat tacttttctg gacatgctga cgccgcagtt
     1921 gatccgtatc accatcgaca acgtgctggg acagctgccg ctgaacgtgc ccgcctttct
     1981 ggaggctgcg tggcttcagc tgggcggcgt ggaagccctg cgcacccacc tgtggtagat
     2041 cgcgctggcg gtgatcgcca ttgcactggt gatcgccttc ttccgctacg gcaacatgac
     2101 gctgaccacc aaggcttccg agactttcgt gcgcaccatg cgcaacgatc tgttcgccca
     2161 catccagcgt ctgccctttt cgtggcatat gaaaaaccag accggcgaca tcatccagag
     2221 atgcacgcag gatgtcgacc tcatt
//
LOCUS       DAWAEA010000228         2245 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6726_length_2245_cov_1.386758, whole genome shotgun sequence.
ACCESSION   DAWAEA010000228 DAWAEA010000000
VERSION     DAWAEA010000228.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6726_length_2245_cov_1.386758"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          381 a          745 c          639 g          480 t
ORIGIN      
        1 ccttcgatag agtcattctg tctgcccgtc ctgccaagtt agaagaaaag actctgcaac
       61 gcgttcgccg catggaagct cccattcatt cctgcgtatc cggcggcaga tattttccgg
      121 cttcgctttc ttccagcgct tccacactgc ggaggcgctt ttcgatgctg cgagtgcgca
      181 caaaggcact gtcgatgctg tcgctggcct gacgaagctt ttcctgcgtg tgctccagca
      241 ccttggcaaa gttgccgaag tcggtcttga ccgcccccag cagcttccag acctcggcgg
      301 agcgctgctc gatcgccagc gtgcggaagc ccatctgcag gctgttcagc agtgccagca
      361 gtgtactggg ccccgctacc agaatgcgct gctcccgctg cagggtctcc actacttccg
      421 cgttgcgcat gacctcggca taaaggcctt ccagcggcag gaacatcacg gcgaaatccg
      481 tggtataggg cggaacgatg tatttgccga tccgcttgcc ctcggtgcgc acggcgttca
      541 tcagggcgcg ccgagccgct tcgcaggctt cgccgtcgcc cgcatcggcc gcttccgtca
      601 gccgggcgta atcctcctgc gggaatttgc tgtcgatggg cagatagacg ggccggtctt
      661 ccttgcccgg caggatcaca gcgaactcca cccgctcctg tgagccaggg accaccgcca
      721 cattccgcgc atactgcgac gaggtcagcg cctgttccag cagtgcgccc agctgcatct
      781 cgccccatac gccccgggtc ttgacgttgc ccagcatccg tttcaggtcg cctacgccgc
      841 tggccagcgt gtgcatctcg cccagagact tatacacctg ttctagccgc tggctgacct
      901 gcgcaaagct gtcgttcagc cgcttttcca gcgtgctgtg cagcttttcg tccactgtct
      961 ggcgcatttc ttccagctga cggcggtttt cgttggcaag gcgctgcacg ccttcgtcca
     1021 aggtgcggcg cagggactcc atacgggcgt cgttgcgctc caacaggctg ttgagctgat
     1081 caccctgcat tttcaatacg ttggacagaa gggagatttg gccgtcgctg gtgcgctgca
     1141 tggtctcgga cagttcccgc cgctgggccg cgctttgctc cagcaggtcg tcctccatgc
     1201 cttccagccc cttccagagc tgctcctgaa agcggcgaaa ctcctgccgc tgcctgcgat
     1261 gaccctgcac caccagcgcg atcagcacca cagccagcac gatcaaaacc agcgccagtc
     1321 cgcctaaaat cagcgtcgaa accgaagcgt tctgcatctt tggttccttt ctgcatcccg
     1381 gattttggtt cggaatgcgg taaataggaa tggccgcgca atgcgcggcc agaagaagtt
     1441 tacatttccc ggcggccctc catggccttg gaaagggtga tctcatcggc gtattccaga
     1501 tcgctgccca ccggcacgcc gtgggcgatc cgggtaacgc gaatgcccat gggcttgatg
     1561 agccgcgcca gataggaggc cgtggcttcg ccctcgatgt ccgggttggt ggccaaaatg
     1621 acctctttca catccggatc gttcaaacgg gccatcaatt ctttgatgcg gatatcctcc
     1681 ggccccacgc cgtccatggg ggaaagagcg ccgtgaagca catggtacag cccttgaaaa
     1741 tcccgcatcc gctcgatggc ggccacgtcc cgggggtcgc gcacgacgca gatttggccg
     1801 ttatgccgcc gttcgtcctg acagatatcg caaagctcgt ccgtggaata attgccgcac
     1861 acgctgcaat accggatgga tttgcggccg ctgtagatcg ccgtcgccag ctcccgcaca
     1921 tcttccagcg gcatactgat gatgtgatag gccagccgct gggcgctctt ggtgcctacg
     1981 cccggcagac gggacagctg cgccaccatt ttggcgatgg gttccagctg cgccatggct
     2041 tagaacatgc ctcccatgcc gggagccagt ttgcccagct cgctctggcg ggtctcctcg
     2101 gcggtgtgca gggcctcgtt caccgcggcc atcaccatgt cctgcagcat ttccacgtcc
     2161 tcgggatcca cgcactcggg cttgatctgg atgccggtca cttcatgctt gccgttgacg
     2221 gtcacactga ccatgccgcc gccgg
//
LOCUS       DAWAEA010000229         2233 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6772_length_2233_cov_1.481635, whole genome shotgun sequence.
ACCESSION   DAWAEA010000229 DAWAEA010000000
VERSION     DAWAEA010000229.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6772_length_2233_cov_1.481635"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1626..1635
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          355 a          714 c          635 g          519 t
ORIGIN      
        1 tgaatccttc cgcaccgtat cgccgcttct gttctgctcc ctgtgtaccc tgctgactgc
       61 cgcttccatg ctgggctttc tggatacgcc gctgtacagc ttttccggct cgttcctgaa
      121 gctgccccgg tacaccctgc aatggttcct gatggcctgt gtgatgatct gcgccgcgct
      181 ggctgcgctt tcggacgttt ggcccaagct gtgcgactgg gcaaagcact ggctgcgccg
      241 ccgtcaatgg cgtcttcccg ccggaatggg ttccttcctg cgtcagcttc ccgtatggtt
      301 ctgcgcgctt ttgtgcgtcc tcggcatggt gcagggtacc aaacagaccg gctacgccgc
      361 cagcttttac cgcagcagcc ggaacgtgat ggaaaacgaa gacatcctgc tggacaacgg
      421 ctttcggaag cgctacggcc tgatgatggc ggtagcagac gcgctgccgg aggacgagac
      481 cattttgatc acgcagtccg ggtatcagta tccgctgaaa ggcaaaggct accttctgca
      541 aagcaatcct attgccccca ttctgaatat gacggcagaa gagatccccg gcgaactgga
      601 acgtctccgc gtgggtgttg tcatcaccag tccttccttc tgggatgacc ggtatctgat
      661 ccgttccacc ctgagcgact atctgagcca gctgcccccg gagcagatcg ttgaaacgga
      721 aacgcttcgc ctctatctgc tggatccctc gctggttccc atcgcgcagg ccgcgctggc
      781 agcgcaggat tgacgtgcgc accgctccgt gctatgcttt gcccgttcct ctgagtgacc
      841 cattcttgat tttgtggagg tttgcatgaa ctgtcccaaa tgcggttcgc cggtaacgcc
      901 cggcgcgccc ttctgtcccg tatgcagcga acccctgcag cagccggtcg gcgcgccgca
      961 gggctttccc aacgatccca acgcagtcta tggccaacag cccgtttatt cccagcagcc
     1021 ggtgaatccg ccggtcaacg gctatcccaa cggctacggc ggctatcagg ccggccagca
     1081 gcccggatac cagccaggct atccgcctta cacccagcag acctacccca ccggctatca
     1141 gcagccctat caatatggag agatccggca aaatcccttt tccagcgctg tcagcgagct
     1201 gccccatgtg ttctccctca gcttccggaa tccgggagag gcgttccaca gtctgctgga
     1261 acggaacgac acggtcacag ggccccttct ctgcggcgtg acgcttctgc tcagcttttt
     1321 tgccggaatg accgtggccc ggggtctggt cagcgtcttt ttctccgtgg tttcttcgct
     1381 gacggggatt agcctggccg ggagtgccag ttccatgaat cagggcatca actatgtagc
     1441 cggacgcatt gcgccttccg taggcggcat tacggtgctc tgccagcttt tgtccatgct
     1501 ggtgccgctg ctggtctgct gcgtgtacct gatgggcatc tgcaaggtgt ccttctcctg
     1561 gccggtgatt ttggggctgg tgtgcattcc cagcattccc tcggctgtgg ccgcgctggc
     1621 cggaannnnn nnnnnacgtt tgtctgcatc ctgtgcggga tggcgttcag ctatctgcag
     1681 ctgggcgcgc tgctgcatta tgtcaccggc cgctccgagc agcagctttt cctgcccaaa
     1741 accgcctgca ttctgatctc cctgctgctg accctgcttt tgttcctgct ggtgggcggc
     1801 ggtttgatgc ggaatgtgct gaactacatg gttcagttgt tatccaacgt gggggcactg
     1861 ctgtgaagcg ctttcacagg cgctggctct gggcggcaat ttcccttctg ctggccgtgg
     1921 ccgcctcgct gtttctgctg cttcgtccgc agaatgcgcc gctgtccgct ccagccccgt
     1981 ccgctgcgct tctgcaggca gacgatgggc tggacgtgat cgaccttcag gcggaatttc
     2041 tgcccgaaag ccgctccctg cgggtcacgc agcgcatgac gctgacctcc cgcgtcagcg
     2101 aaagccggga tacgctggtg ctgcgcacct ggcccaacgc ctatcagagc atggaaacct
     2161 ctccattgac cctgaccgaa ttatacgacg cggcttaccc ggacggtttt tccaccggcg
     2221 cgctggtgat gga
//
LOCUS       DAWAEA010000230         2218 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6819_length_2218_cov_2.305594, whole genome shotgun sequence.
ACCESSION   DAWAEA010000230 DAWAEA010000000
VERSION     DAWAEA010000230.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6819_length_2218_cov_2.305594"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          341 a          671 c          663 g          543 t
ORIGIN      
        1 atatagtcca catcgggctt ttccatctgg cccagaaaca tccgggcgta ggagctcagg
       61 ctttccacat agcgccgctg gccctcggcg taaatggtgt cgaaggcaag actggtcttg
      121 cccgagccgc tcaggccggt gaacaccacc agcttgttgc gggggatctc cagcgtcaca
      181 tttttcaggt tatgctctcg ggctccccgc acaatcagtt tatcctgcaa aatagccacc
      241 tcgtctgttc cttcggaatc ctgtttcaaa cgcgaaagaa tgttcgcatc aggtattcta
      301 ccatagggtt tttgatcccg caagaagttt ccggttttca agggatggtt ttttctgcta
      361 taatgaacgc ccgggcttcc cttttttccg ggagccccga ttcccctgca cagaaaggaa
      421 gcagcgccat gcgagaccgt tccgtagacc tccagcgagg cattctggtc gtcctgatgc
      481 tctacgccca ccttcttcag tttttcggcg acctccagcg gtttccgtcg attcatttct
      541 gggtgatggg aatcagcctg ctggcctttc ccgccttcgt cttctgcttc ggcaggacgg
      601 cggcgctggc ctatctgaaa aagcccttcc ggcaggcgct gccccgcatg gggcgcacct
      661 tcgggcgctg tctggctgcg ttttatgtat ccggcgcggc cttccgggtt ctgcgggagc
      721 atcggccgct ggaggccgaa acgctccggg gcattctcct gctcagcgat attcccggct
      781 ggtcggaatt tctgatcgcc ttcgccctgc tgatgctgtc ggccatcctc ctgtttcccc
      841 tgctgcggca gctcgcccgg cggcgctggc tctggctgcc gctgagtctg ccctgtgcgc
      901 tgtgctgctt tcttccctat gaccgcgtca cgcttcccca gctggccctg ctgataggcg
      961 gcaaaaatct ggtgacctac ccgatcgtgc aatactttcc tttctttctg gcgggcgtgg
     1021 cctatgcgga cgcgagcttt cgtgaacggc tgggcatgag cggcgctgcg ctggccttca
     1081 gcgccgcagg cctcgtccgc tatgtccgcc acggcctgcc ggagcggttt ccgccccact
     1141 gggcgtggat tgtcctgccc tgcttcgcca tcgccctgct gacggcgctt tgctacggtc
     1201 tgtgccgcct gcccggcgaa aaatactggc tgcccgtgga atggctgcgg cgggcgctgt
     1261 gccgtctggg cggcgcgtcc ctgtattatc tggtgggcag caatctggtg ctattcacgc
     1321 tggcaggaag tgagattgct cccgtttccg ggcgtttctg gccgtggaat cagtcggtcc
     1381 agtcccctgt cggggcggtc agctggctga tcgtgctgct ggcgcttctc tggttcgtca
     1441 cccgtctggc cgggcgcagc tttgccgccg aagtgcgcaa acgataagcc atatttctat
     1501 ttgacaacct ttttccatca tgctatcctt tcagcgcggc agtgttttgc cgcgctgcta
     1561 tttttaggaa gaagggcgga tgcgcatgat cggcgttctg gattacggca tcggaaacat
     1621 cggttccatc ctgaacatgc tcaaaaaggt gggagtgaaa gccaaacctg tcaccacggg
     1681 tcaagcgtta tccacctgcg accggctgat tctaccgggg gtcggggcgt tcgatcaggg
     1741 catggcgctt ctgaaccaga gcgggatgcg gggcgcgttg gacgaagccg tggcttccgg
     1801 caagccggtt ttgggcattt gtctcgggat gcagatgctg gggcttcgca gcgaggaagg
     1861 cgtatcggac ggccttggct acattccctt ttccatgcgg cgttttcgtc tggacgatca
     1921 cccggagctg aaagtgcccc acatgggctg ggatcgggtc gagattgtcc agcgagatgc
     1981 ggcgttggtt caggggttag agcagcccgc ccgtttttat tttgtccaca gttactatgc
     2041 ggtctgcgag gacgagcgcg acgttctgat ggaatgcgac tacggcgttc attttgctgc
     2101 ggccgtccat cgtggcaacg tctggggcgt tcaattccac ccggagaaaa gccaccgttt
     2161 cggtctccaa ctcctaaccg ccttcagcag ggggtgaccc cctgcacccc ggactgcg
//
LOCUS       DAWAEA010000231         2213 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6832_length_2213_cov_1.747451, whole genome shotgun sequence.
ACCESSION   DAWAEA010000231 DAWAEA010000000
VERSION     DAWAEA010000231.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6832_length_2213_cov_1.747451"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    243..486
                     /estimated_length=244
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    803..812
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1572..1581
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          414 a          556 c          583 g          396 t
ORIGIN      
        1 ggacggcggc atccgtgggc tggtcgttca tctgggagag cgtttttccg aggtcagcga
       61 ccccgcgccc ctgccggacg gccgcttcgc catcaccgcg caggcgggcg cgtccctgca
      121 gaagctcagc aaggcggcgg cggatcacag cctgaccggg ctggaatttg ccgcgggcat
      181 tcccggcacc gtgggcggcg cggcctatat gaacgcgggc gcttacggcg gtgagctgaa
      241 agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnngatc gccgccgtac agaaggcggt gcaggaggac agcggcgtat ggctggagcc
      541 ggaggtccgc attctgggag aggacgcgcc ggtttccctt ctgtaaagcc cagtcaatac
      601 aaacgaggtg caaaagtatg cagtacctga tcctgaccgg gatgagcggc gcgggcaaga
      661 ccgtcgccat ccgttatctg gaagacctgg gcgcgctgtg tgtggataac ctgccgccca
      721 tgatgctggt gccctttatg gaggcctgtc agaacaccaa tctgcacaag cccatgatcg
      781 ccatcgcggc ggacgtgcgc tcnnnnnnnn nngccgtgac ccgcatgatc gacgaggccc
      841 ggctggtggg tttccacatc gacacgctgt ttttcgaggc cagcgacgag gtgctcatca
      901 accgttacaa ggagacccgc cgggagcatc ccctgagcgg cgagggcctt tcgctggaag
      961 aagccatccg ggaggagcgc agccgcctgc agcccctgaa ggaagcggcg aaccatctgt
     1021 tcgataccag caatctgcgg ccccgtgccc tgcagaagat gctcagtgag attgttctgc
     1081 atgccggaag cgagtctccc ctgcgggtgg aggtgctcag cttcggcttc aagcggggca
     1141 ttccccggga aggcgatctg gtcttcgacg tgcgcttcct gcccaatccc ttctacattc
     1201 ccgagctggg ccaccacacc ggactggatc aggatgtgaa ggactttgtg ctggggcacc
     1261 ccgtcacgaa agaatttctg gaaaaatgca tggacatgct ccgcttcctg ctgcccaact
     1321 acatcaacga gggcaagcat cggttggtca tcgccatcgg ctgtaccggc ggggcgcacc
     1381 gcagcgtggc catcgcggag tatgtggccg ccgagcttca gaaattgggc tatggagtca
     1441 acgtcaacca ccgggacatt gcgctggagc aggctcactg ggccgcccgc agcgagtatc
     1501 agaagggcga gccgtaacgt ttcggttttt caaccccgtt tttccgtcca tcttgcggaa
     1561 aagcggggtt tnnnnnnnnn naaaaatttt gtcaaaaaat gtcaaattta tttcgctgca
     1621 tcaatcgaaa cctaccatga aatagcggat tcgttgacaa gtcatgacac acatgttata
     1681 cttgcaacag ttttcaggcg aaatgaggcg gcattttacc ggcatttcaa gtcctgcaag
     1741 ggcatacggc gtttcaatgt tccttaacat tttgtaatat atcaatttca tattccgtgc
     1801 agggaagggg tatattacac gatgaacagg caatatggag gcgtcggtat tggagaagca
     1861 gcttaaaatc gtatactact acaacagcgg gttttcggtg caggtgggca gcacgctgtt
     1921 catcttcgac tactgggaag gcgaaaatcg gagcctttcc gcgtcagtcc gcatccggcc
     1981 ggagttgttg aaggcctacg aacgcatcta cgtcttcatc agccacgctc accccgacca
     2041 tctggatccc gtggtataca cgtgggccaa ggaagggctg cccattacct acatcgtttc
     2101 cagcgatatg cccatcggca ccatcggcaa acggctggct cccctgcagg aaaaacagtt
     2161 gacgcaggac atctgggtca aggcctatca gtccaccgat ctgggcgtgg cct
//
LOCUS       DAWAEA010000232         2206 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6863_length_2206_cov_1.963738, whole genome shotgun sequence.
ACCESSION   DAWAEA010000232 DAWAEA010000000
VERSION     DAWAEA010000232.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6863_length_2206_cov_1.963738"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    580..589
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1207..1336
                     /estimated_length=130
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1582..1591
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          469 a          588 c          656 g          343 t
ORIGIN      
        1 ggtggaggca cagaatccac agccgataca ggcattgttg acacggtatt tcataaaaca
       61 ttgctccttt cctgaagccc atgaatggga ctttcaggga aaggatgccc cggcgaggca
      121 aagctcatac gcacggtcag ccggggcggc gaagcgtttc cacaggctcc tgccagagcg
      181 cgtaaagatc gttcatccag ccgtacacgt ccatgctata gaccttttgc aggttttccg
      241 gggtgaagac cgcttcaatt ggcccctgcg ccacggcgcg gccctcgttc atcagcacgg
      301 catgggtgcc gtaccgacgg gcgatgctca ggtcatgcac caccgacaac gcggcccggc
      361 ccggcgtttg cagccattgg ccgatcaggc taaacaggct ttgctggtac accaggtcca
      421 gatgattggc gggctcgtcc aacagcagaa tttccggctc ctgagcaagc acctgagcca
      481 gaaaggcccg ctgcacctcg ccgccggaca gttccagcag acttcttttt cgcagcagct
      541 tcatgccggt cagctccaac gcccgttcga tcgcgtcggn nnnnnnnnna cgcaaattcc
      601 gcccgtttag acgaatttca ccttcatagg gcacgccttg ggcgatggcg gagatcagcg
      661 aacttttccc cgcgccgttc ggcccgacca gcatgagcca ttggccctcc tccagcgaaa
      721 aggaaagacg atccaccact gtatgcgcgc cgtaacgcac cgtgatattt tccgcattca
      781 gcatggttta tccgtccttt ctgctgcggt agaagatgac cacaaatacc accgagccga
      841 tcagagacgt gaccacgccg atgggcagct caatggggga aagcaccgtg cgggcggtca
      901 ggtcggccag cagcaggaat accgcgccca gaaacaggga ggcaggcagc agccgtttat
      961 ggttggggcc ggtcagcatc cgggtcatat ggggcgtgac cagccctaca aagccgatgg
     1021 tcccgccgat ggacacgcac gtgccgatca gcaccgagac ggcgatcatg atctccagct
     1081 tgacccggcg gacggaaacg cccacgtgcc gggcattttc ttcgcccacg gcaaaggcgt
     1141 tcagttcccg gccccgggac aggatccagc cgccgcatgt cagcagcgcg acgcccagca
     1201 aagtggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnttgc tcccggccag cgaacccatg gtccagaagg tgatggtgcg
     1381 cagtttttct cccgaaaagg cgatcagcag gctgatgagg ctggacgcga acatggagaa
     1441 aatgacgccg atcagaatga tgctgttggt gctcaggctg cggtcaaggg cataggcaag
     1501 ccccaggatt atcagaagtg acagaaacgc gaagacgatc gccgtcacgg cggtgccgcc
     1561 atagggcagg ccggggaagg tnnnnnnnnn ngttgtcggt cggcgcgcgc caaaagttgg
     1621 ggcgcagcgt ggagccgtcg gccagcgggt tccgcaaaag tccctgcagg gctgcgccgc
     1681 acagggagag agcagcgccg gtcagcgcta cgcatagcac ccgggggagc cgcacggaaa
     1741 aaacaacggc tttcgggaat ccctccggca aaggcagccc ccagagggcg ttccacaggg
     1801 cggcaagggt agaatctatg ggaatggaca cgctgccgat gcaggtgcaa aggagcatgg
     1861 taaaaagcaa aatcagggtg gcggcgagcc acagggggaa gaaacggcgg gggggtttca
     1921 tgagggaacc tcccggaaag agtaggggtc agtgcgctga aagcagcgcg gagtggaaag
     1981 aggtgttcca tggtgcttct ttgttttgat agttagaaaa aggatggcaa acaacaggcg
     2041 gagcgcacgg cagtatgggc tgtgccgatg attaccctga ggaaagtgtc taatgcatcc
     2101 gtagacgcat catctctgtc ccgatggggg cccgaagggg gcagggggcg aaagctgctg
     2161 cgtaagacaa ccctgccgta aggaaacccc agctggggga atgacg
//
LOCUS       DAWAEA010000233         2205 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6870_length_2205_cov_1.678605, whole genome shotgun sequence.
ACCESSION   DAWAEA010000233 DAWAEA010000000
VERSION     DAWAEA010000233.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6870_length_2205_cov_1.678605"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    600..609
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1360..1416
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          483 a          585 c          639 g          431 t
ORIGIN      
        1 ggagtcagct acatggagaa cagtgtgggc tggcatggca aggcccccaa cgacgaggaa
       61 tacaagattg ccatggagga gctgaacgca cagctgacgg aactggaggc acaggaatga
      121 gtgaggtgaa aaagatcgcc acccgcgaca gctacggcgc ggcactggtg gaactggcca
      181 aggaacatcc cgacctggtg gtgctggacg ccgacctggc cgccgccacc aagaccggcg
      241 tattcaaaaa ggtatacccc gaacgtcatt ttgactgtgg cattgcggaa agcaacatga
      301 tggccgcagc tgccggcatg gctgccatgg ggctggtgcc ctttgcgtcc agctttgcca
      361 tgttcgccgc gggccgcgcc tttgaacagg tgcgcaactc catcggctac ccccacctga
      421 acgtcaagat cggcgccacc cacggcggca tttcggtggg ggaggacggg gcttcccacc
      481 agtgctgcga ggattttgcg cttatgcgct ccatccccgg catgacggtg ctttgcccct
      541 ccgacgatgt ggaagcccgg gccgccgtca aggctgccta cgagcaggaa ggtcccgtcn
      601 nnnnnnnnnt cccgtctatc tgcgtttcgg acgcctggcc ctgccggtgt tccacgacga
      661 agccaccttc cggttcgaga tcggcaaggg cgaacagctc accgaaggga acgacgtggc
      721 aatcctggcc accggtctgg aggtgaacga ggccctcatc gccgccgaac agctgaaaag
      781 cgagggcatc cgcgcccggg tcatcaacct gtgcaccgtc aagcctctgg acgaggagat
      841 cgtagtcagg gcggcccggg aatgcgggga acattccatc ttcggtggcc tgggcgaggc
      901 ggtggccggt ctgctgggcg aacagtgccc cacccccatg cgccgggtgg gcgtcaagga
      961 cgtgttcggc cactccggcc ccgcctggga gctgctggaa cagttcggcc tgcgcagcga
     1021 tgccatcgtg gctgccgtgc acgatgtgct gcgctgacca gctccggccc ttcaccctct
     1081 gtgcgtcgat tctggccgca gattttgccc atctggcgca ggatgtgcag gccgccgacg
     1141 ccgccggtgt agatgccttc cacatcgaca tcatggacgg ccactttgtg ccggccatct
     1201 ccttcggcac cggtatggtg cgcaccatgg gccgcctgac cggcaagcct ttggatgtgc
     1261 atctgatggt ggagaatcct ctggactttc tgccggaact ggaggagctg ggggtaaaga
     1321 gggtctcggt ccattacgag atcccgggcg ggccggaagn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntatc tgcccgggag caacaaaaaa
     1441 atcgccgccg cccggaccct gctgcgagag agggagagtg tggctgtgat tcaggtggat
     1501 gggggaatta cctccgttac actacctggt gcctatgcgg cgggggccag aagtattgtg
     1561 tcgggaagca ctatttttag tggagccata gatgccaacg tggtctcttt gcgacagaca
     1621 ataggaaaaa acacataaac ttatattgta agaaacggaa ctacttacaa aagatatcta
     1681 actaaataga ttcaaaccaa aaagagtgct gactaaaata cagtcagcac tctttttggt
     1741 ttgctatcat gtgaagaatc atcggcaccg attacgatac tgtgcggggg ttatttggtg
     1801 aaattggcga aaaaccgaag taaagtgact ttggttggca tatcctaaat agtgtctaca
     1861 caagatcagg tcaaaacagt acaccaaata gcaatgcaag taacggtcat actcccgctg
     1921 ctgcttgcga tttcgtttag ccggaatgac gatattcatt ccagcatcag cggcataagc
     1981 aatgatttga ttggtgtcat acccacgatc ggccagtaat gtttccgccg ataatccatc
     2041 aatcaagtga atagcttctt tgcaatcagc tcgggtaccc tctgtgataa gtactcggac
     2101 cggcatacca tttgcatcca cggcaaggtg aatcttggta aacactattt agggggagag
     2161 gtgtggagag ggggacaagc aaaaaaccgc tcagttctgg catta
//
LOCUS       DAWAEA010000234         2203 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6878_length_2203_cov_1.819832, whole genome shotgun sequence.
ACCESSION   DAWAEA010000234 DAWAEA010000000
VERSION     DAWAEA010000234.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6878_length_2203_cov_1.819832"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1085..1136
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          376 a          665 c          631 g          479 t
ORIGIN      
        1 tcaaagtagc ggatgggcag ggtctgcata tgggcgaaca tatcgtcccg cacgtttttc
       61 aaaacgccct gcgccaccac caccatcagc cggttgtaca ggaagccgca gaccatgccg
      121 gtcacgtaaa tgcaggccat gtacaccagt tgggtcagca ggccggagaa gtccgggttg
      181 atctggccga tcatgggggt gatgtagtcg tcgatcaaag tcttgatgaa ggtggaagcc
      241 gtcacgctgg caatggtgtt gcccagaatg cagaacagca ccaaaaccag acgcaggcgg
      301 tagcggccca catagcccat cagccgggcc acggtggctt tcaggttctg cggcttcgcg
      361 ccgcccctgg gtccgcgggg tcctcgcatc attctgcatc gcttcctttc atctgggatt
      421 cgtatacctc gcggtaaata tcgctggtct tgagcagctc tcttggcacc ttttcaccgg
      481 cgcagatcat ggccagcagc tccttttccg cgcggacata ccggtcatcc tccctatttt
      541 tcccgtagga ggaaacggta ttcgcctccc tttgcggctc ggccttcggg gcgcggcgct
      601 tgccgacctg ctccatcagc gtctcttcgg aaaagccggt ttcgatggtg agcctgcgca
      661 cgaagcgctc gacctcgacc gggtcctcct gccggcgcag cacctgcgaa gcgtccacgg
      721 cgtatttggc gcgggcttcg gcgttggaaa gatcgtattt tgccgccagc gtatccagcc
      781 ggtaggtggt ggggtcgatg gagcccaaat cgcttagggc ctgtccgccc ttggcgcgga
      841 cgtattcgtc cgggtccatt ccgccgggca cgacggtcac gcgcgcgggg acgttttcct
      901 gctcaaagag gtcaaggccc cgggcaatgg cgcgctggcc tgcgccgtcg ccgtcgtagg
      961 tgatgagaat ctgcggggcg tagcgcttca tcagccggat ctgctcgttg gtcagcgccg
     1021 tgcccagggt ggccacgcac ccgtccacgc cgtgctgata gagcgatacc acgtccatat
     1081 atccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaga
     1141 attcaaatat ttgggctgtt cgtctcccat gacgcgcccg ccgaaggcga tcacgcgccc
     1201 gcgggggtcg aagatggcga acatcacgcg ctcgcggaag gcgtcaaaca ccttgccgcc
     1261 ccgctccagg gcgaggtttg cgtcgatgag ctcctgcttg gcaaagccca ggtcggaaag
     1321 atgggtatac aggctcgacc agcccctggc cgttgcgccc aggccgaaat gccggatcac
     1381 gttgtcgcgg atgccgcggt tgtggaggta ttccagcgcc tgcgcgccgt cgggcgtata
     1441 cagggttttg tgatagaatt ttgcggcctc gaccagcgcg gcatagagcc ggtcgcgatc
     1501 ctttttcggc cccgtgtcct cgccgcgctg caattccggt atgggcatgt gcgcgcgctc
     1561 ggccaggtgc ctgaccgcct cggggaattc catgtgctcg atgttcatca caaaggtgat
     1621 gacgttgccg cctgcgtggc agccgaagca atagaacatc tgcttttcct gatcgacgga
     1681 aaacgaaggg gttttctcgc cgtggaaggg acagagcccc caatgcttgg cgcccttctt
     1741 ctgcagcgtg acgtattcgg agacgaccga aacgatgtcg gatttatcgc gcacttcgtc
     1801 gatccactcg ctggggaacc tgcctgccat gcgcttcctg tctcctttcc gccgtcgatc
     1861 ctgctgcccg cccattcaca ctttgttcgt tgtttgaaaa acgaaccgat agttatattc
     1921 tctggttttt ctgcaaatcc ttctgctttg cgcttttttc tcacaaaaaa agcgtctctt
     1981 ttcccttgct tcgtcaaaaa gctgaaagat tcggcatttt ggcgtttcgg gatattctgc
     2041 ggagagaaag ttccttcccg gctgttttct tctcagcggt gcgcgcatcg cctccccttg
     2101 tgtcagggga gtcaaagaga ccattctccc ccctgagcgg ggggagctga cgctcccccc
     2161 tgcacccccc tctcccgccc ccctgacggt tacagacgga cag
//
LOCUS       DAWAEA010000235         2201 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6888_length_2201_cov_2.372787, whole genome shotgun sequence.
ACCESSION   DAWAEA010000235 DAWAEA010000000
VERSION     DAWAEA010000235.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6888_length_2201_cov_2.372787"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          371 a          699 c          670 g          461 t
ORIGIN      
        1 catttgtggt agctcagcag aagattcacg tccgcagcgt aatccgccag cggattttcc
       61 acaaactcat gttctttcat gggatgaggc agacagcgct cctgcccgca ttgttcctcc
      121 ggctcgtaga gagaagaaag cagcatgtac aggaacaccg cgtcgtagct cagggtcatc
      181 cggccggccg cgccgtagcg ccggcccaaa ttgcggcaca ggccgcagta gtaggcacga
      241 tagcgtttcc ggcgttcttc cggcatttcc gccgaagcgg tcacatagcc gaacatcagt
      301 gtttctcctc atgagagtcg ctgaaatccg cgtccacagc gccgtcggcg tcgtcggcgg
      361 cgctgttgtc cgtgtagcgg cccactgcct gcagggcgtt ggccagctcc gccatgcggc
      421 gcttcagctc gccgtcatcc gtgccctgca tggcctgttc cacctgcttg cgcagacctt
      481 cgatgcgttc cttgtcctcc cggctcagcc tgcgctgggc gtttttggat tcgtaaagca
      541 gctgctccgc ctgattgcga acctcggcgc tctccttgcg gcgcgcgtcc tcggcggcgt
      601 agcgttccgc gtcccgcatg gcctgttcga tctccgcgtc ggacatgtgg gtggactggg
      661 tcagggtgat ctcctgatgc ttgcccgtgc ccaagtcctt ggcggacacg tttacgatac
      721 cgttggcgtc gatggagaac gtgacctcga tctgcggaat gccccgcagc gcccggcgga
      781 tgccggtcag gcggaagcga cccagcgtct tgttgcagct ggcgatctcc cgctcgccct
      841 gcagcacgtg gatgtccacg gaggtctgga aattggcggc ggtggtaaac acctgactgc
      901 gctgcaccgg cagggtggtg ttgcggtcga tgatccggga gaacacgtcc cccacggtct
      961 caatgcccag cgaaaggggc gttacgtcca gaagcagcag ccccttgacc tgacgggaca
     1021 gcacgcccgc ctgcaggcac gcgcccatgg ccacgcattc gtccgggttg atgtccttgc
     1081 tgggctcctt gccggtcagc cgcttgaccg ccgcctgcac ggcggggatg cgggtggagc
     1141 cgcccaccag cagtacccgg ctcaaatcgc gcgcctgaag gccagcgtcc cgcagcgcct
     1201 gctccaccgg gccgacggta gcttccacca gatgggcggt cagctcgtcg aacttggccc
     1261 gggtcaggtc gatctccaga tgctttgccc cgtccgggcc ggaggtcagg aacggcaggc
     1321 tgacggtggt ggtcatggcc gcgctcaact cgatcttagc cttttccgcc gcctcccgaa
     1381 tgcggctcat ggcggtctgg tcgtgggtca ggtcgatatg ctcccggcgg cgaaactcct
     1441 ccaccagata gttggacacg cacgcgtcga agtcgtcgcc gcccagccgg ttgttgcccg
     1501 ccgtagccag cacttcgatc acttcgttgt tgatgtccag aatggacacg tcgaaggtgc
     1561 cgccgcccag atcatacacc atgatgcgct gggaaccgcc cttgtccacg ccgtaggcca
     1621 gcgccgcggc agtgggctca ttgatgatgc gcagcacgtt cagtccggcg atgcggcccg
     1681 cgtccttggt ggcctgccgc tgaccgtcgc tgaaataggc gggcacggtg atgaccgcgt
     1741 ccgtgacggt ctcgcccaga taggcctccg cgtcctgcct gagcttttgc aggatcatgg
     1801 cggaaatttc ctggggcgta tatttcttgc cgtcgatgtt tacccggtta tccgtaccca
     1861 tctcccgctt gatggaaaaa atggtgcggg atccgttggt cacggcctgc cgcttggccg
     1921 ccgcgcctac gatacgctcc ccgtttttgg taaaggccac cacggacggg gtcgtccgtc
     1981 cgccctccgt gttggggatg accacggctt cgccgttttc catcgtcgca acgcaggagt
     2041 ttgtggtgcc aaggtcaata ccgatcagtc tgctcatagg agaaaagact cctttcctaa
     2101 ggttctttct tatcataacc tatattttcg gaaaaagcgt agcgtaaaaa tgaagaaacc
     2161 gtaaacaata cgccttggga gggaaaaaac gtgaatttgt a
//
LOCUS       DAWAEA010000236         2200 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6895_length_2200_cov_1.527273, whole genome shotgun sequence.
ACCESSION   DAWAEA010000236 DAWAEA010000000
VERSION     DAWAEA010000236.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6895_length_2200_cov_1.527273"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1210..1403
                     /estimated_length=194
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1911..1920
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          420 a          560 c          682 g          334 t
ORIGIN      
        1 ccgtctggcg gacggcgtat atatctacct gcggctctgg acggatcgaa gctttcagca
       61 tttggctgtc cgggaactga aaaagctgga cggccccttc gacgtggtgt tttccaccta
      121 tgcgccgttg agcgtccatc aggcggcata ccgggcgaaa aagtccggtc ttgcgcggat
      181 gtggatcgcc gatttccggg acgaggtggg aatgcccttc cgctggctgg agcccttcca
      241 tcggcggtat atgcggatgc tgcggaaaaa cgccgacctg ctgactacgg tttccaaagg
      301 aatgctggac gcgatcggca ttagcgaccg gggacgaatg ctgtccaacg gctttgaccg
      361 ggaggacttg ctggaggacg aaacggcacg gcaggaggac ggccgcttcc gggtcgtcta
      421 ctgtggcacc atcgaaatgg gccgcaaggg tattccgccc cgggatctta cgcccttttt
      481 ccgcgcgctg cggggcgtga tcgatcaggg aatgttgaag cgcgaacagc ttcggctggt
      541 ttacgcgggc cgggagggtg cgctgtttac ggactacgcc cggcagtgcg gcctgaagga
      601 ctgcgtggag aaccacggcc ccgtctcccg ggaggaatcc ctccgcttgc agcgggaggc
      661 ggatgtgctg ctgctggcct cgtggaatac agcgggccgt cagggcattc tgaccgggaa
      721 aatgtttgaa tacatgatga tgaaccgccc cgtggtatgc tgcgtaggcg gcgaactggc
      781 gggcagcgag ctgaaacggg tgatgacgga aacgggcatc ggtttctgct gcgagcaggc
      841 tgccggggca gccgatcaac aggcgctgga agcctatacg gcggagctgg ttcgccgctg
      901 gcaggaaaaa cagccgctga tgcaggaaaa gaacgaaacg gctgtagaac agtatcacta
      961 tccgcagttg gctcaaaaac tggacgactg gattcgggaa ctggaaaggg aggatgcatg
     1021 atgaagaaac ttcttcgttg gaacgcgctg ctgctggcgc tgtgctgctg cctgaccgcc
     1081 ttcgcgctgg cggaggaaga gccggtggtc atcaccacca gcgtggatac cctgacggta
     1141 gcggtgggcc gcattgtgga ctacgagctg tccatttcgc cccggagcgc caaaaaaggc
     1201 tatacctttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnncagcaag gtcgatccca cggccaccaa ggtattggac
     1441 gtgatcgtgg tgcagccggc caaaagcgtg aaggcccagc tggatcagga gaccctctcc
     1501 gtggggcaga cggcgcagat caccgcttcg gtaagcccgg aagacgcgac cctgcagggc
     1561 gtgacctacg aaagccgcaa gcccgaggtg gctgcggtgg atgaaaacgg cgtggtcacg
     1621 ggcatgagcc ggggcagcgc caccatccgg gtgaagtccg aggacggcta tgtccagacc
     1681 agcgtgaccg tacaggtgcg tcagccggcg gaagaaattc agctggaact ggccaaggcc
     1741 acgctgcccg aaggcaaaac ggcgaccgta aaggccactg tgctccccaa ggacaccaac
     1801 aacaagaaac tgacgtggag ctcctcggac gagagcatcg ccacggtgaa ccgggagggc
     1861 cgcgtgaagg gcattcagcc cggaacggtc accatcaccg ccgccagcgc nnnnnnnnnn
     1921 gtgccggagg taacgggctc catcgacgtg caggtggttc ggctggccaa gtccgtggcc
     1981 tttgagcaaa aggaatacga cgtgatcctg ggtcagacgg cgcagctggc cgttacggtc
     2041 ggcccggagg atactaccga taagtcggtg acgtacaagt ccaacaatcc gaagaaggtc
     2101 tccgtggacg agaacggcgt ggtcacggcg ctggctgcgg gcaaggccac tattacggcc
     2161 accacggcgg acggctcccg ccgtcaggcc acgacggtgg
//
LOCUS       DAWAEA010000237         2189 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6930_length_2189_cov_1.245548, whole genome shotgun sequence.
ACCESSION   DAWAEA010000237 DAWAEA010000000
VERSION     DAWAEA010000237.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6930_length_2189_cov_1.245548"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    390..476
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1547..1556
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          396 a          652 c          597 g          447 t
ORIGIN      
        1 aactacgact atttcagcgg cgttgaatac ggcttcgacc tgcgtcagcc ggtgggcagg
       61 cgggttttcc ggctggaata tcacggaaag ccggtgcagc cggaggattc cttcacgctg
      121 tgcctgtcca gctatcgggc cacaggcgcg ggcgattttc cctattacgc tcagtgtccc
      181 cgggttcggg aaatccagac cgagatcagc gagctgatcc tgaattattt tacggctcac
      241 ccttatgtgg aggtggagga aaagcctgcc tacacggtca ttcgtccctg aaaaacgtac
      301 agaaaaaagc cttccgggaa gcgcccggaa ggctctcagt tgtcgaaaaa ctcctttggg
      361 gagttgtcag cggggccccg cagcccctcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatca
      481 acggctgcgc gccggtgacg gggtccatgt ccatctgcat aacgtccttc atgtactcgt
      541 tccatcggtc caccacggcg ctgcgggcct gcacctgagc ggcggtctgg acgctgtcgc
      601 attcataata gccgaacagc tcgttgccca tgttccagat ggtgtagttg cgaatcccgg
      661 cttccttgag gacggctttc atttccggcc agagatgagc gtgccggtcg acgtactcgt
      721 ccagcttacc gggcaggacg gtggctttcc atgcgtagcg ttccatgacc tgcccctccc
      781 ccctcagcca agaaaagcct gattcagctt tacgccgaac tcgtcggcaa tggccttcaa
      841 atccgcgtcc cggatggtct gaagcacggg ccgcccggaa gaaacgacct tcatataaat
      901 ttccgctgcc ttttcgatgg tgtgcatcaa gccgaaggcg gtgtcgaaat ccggcccgga
      961 gacaaacagt ccgtgatgag cccagaccgc cgcgtcgtat tccttcatca gtttactggt
     1021 ggccagcgca atgtccgcgc cgccgggcac catccacggc accacgccta cgccgccggg
     1081 aaacaccacg gggcactcgg tggcgctctg ccacagggcg cgggtaaagt cgcgggcggt
     1141 cagaggcaga atgtaggtca tggcgatcac attggccgga tgggcatgat agatcacccg
     1201 gcaggctcca ttggtcgcgg tcttgcggac gctgtggttc agaaaatggc tagggaactc
     1261 gctggtgggc ttgccgctct tttccagtcc ccacacgatg cggtagctgt cccctgcctg
     1321 attgatctcg cagatgcaaa gattatcctg cggggccagg atcacattgc gcaggtactt
     1381 gccgctgccg gtggcgatga aatattcccc tttcaggttg tctgcctgta cgcccagttc
     1441 cacccacggg ccgggcgttt tcttgaaaaa tggccggcac tgcgccgctt cttcctccgt
     1501 catgcggtag gtcagattgc cgccgttgcg ttcgtgccag ccctgcnnnn nnnnnntcgt
     1561 tgcacaggcg gatatagccc tgcagtacct ccaaatcgaa aatgctcatt tcagtttctt
     1621 cctttcagca cgtcggcttc gtatttttcc accgtctgga accagccctc gttctccggc
     1681 acgccgcact gacggcagta ttcctcccac accgcgccga agggcagggt cttaagctcc
     1741 tcctgcgcca tcagaagctc ggtgaatcga tcctcgtcct gcaaagttct cagccggtca
     1801 tggggcatca gcaaggccca cagcagggcc ttttccacgt tgcgcgcgcc gatgacccac
     1861 gccgccaccc gattgatgga cgcgtcgaag aaatccagcc cgatcagcac ccgctccgtg
     1921 gcgttgcagc ggacgatctc tgtggcgatc tcccggattt catcctcgaa gcgcaccacg
     1981 tggtcgctat cccagcgcat gggccgggtg atatgcagcg gcagcttgtc gaaatagcac
     2041 agcatggcgg gaatcttgtc gctgacaaat tcctgcgggt gataatggcc gttgtccagc
     2101 aggttgtaca cgccggaacg ccgcgccgca taggcggtgt agaactcgtt gctgcctacg
     2161 gtgtaggctt ccagcccaat gccgaacac
//
LOCUS       DAWAEA010000238         2173 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6977_length_2173_cov_3.317753, whole genome shotgun sequence.
ACCESSION   DAWAEA010000238 DAWAEA010000000
VERSION     DAWAEA010000238.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6977_length_2173_cov_3.317753"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          404 a          723 c          677 g          369 t
ORIGIN      
        1 cggcccgggc ggcagccagc agcacgttga tggtgccgcc cacagtgacc atatcgaaga
       61 atacgtcgtt gcccaccaga tgatcggccc tggcgtatac cacgccgccc cgatcctcaa
      121 tggtggcccc cagcgtttca aagcccttca aatgctggtc gatggggcgg gagccgatgt
      181 tgcagccgcc gggataggcc acctcggccc gtcctccccg gcccagcagc gcccccagca
      241 gataataact ggcccgcaga cggcgggtct gccggtaggg aacgtggtat tccttcacgt
      301 tggccgcgtt gaccaccatc cgctggccgg gacggtagtc cacctccgcg cccagttccc
      361 gcagaatatc caccagcgcc cgcacgtcct cgatgtccgg caggttttcg atggtgcagg
      421 gctcgttgca cagaagcgcc gccgggatca ccgccaccgc cgtattttta ccgccgctga
      481 cggtgatctc ccccaccagc ggattgccgc cggtgatcag cattttgtcc ttgctttcca
      541 taatgcgggc ccctgcgggc cgccctcacc tcttttcgca gtccagaggt tcctgcgtca
      601 ttgtatccaa aaagtgtcag ctttcattat acacgatttg ctgtttcttg gcaaggctgc
      661 ccgcccgcgc cctttgcgtt caggggattc cacgttcccc ggcggaaatg caggctgaac
      721 agcaggcaca gcagcaggtt gtgggcgatc atgcaggccc acgccgcctg aagccccagc
      781 cccagaagcc gggtgcagac gaaggtgccc gcgatgcgta cgccccacat gcccgtcagg
      841 ttgaacagga agggaatcag tgtgcgccca acgccctgca tcatgccctc gatgacgatg
      901 ggcacgccgt agaagggctc ggagacggcc accatccgca gcacggtgct gcccagcgcg
      961 atgaccctct cgtcggagga gaacatcgcc atcaggggcc gggcgaaggc gaacaaaagg
     1021 gccccggaaa cggtcatcag ccccacctcc agcggcagga tggtgcgggc cagccgcttc
     1081 atgcggtcgt tgtcgccagc ccccagcgcg ttgccggaca gggtggcggc ggcggtctgc
     1141 atgccgaagc cgggaatgta gaaggcggac tccaccgtat tggcgatggt atgggcggcg
     1201 gtggccgtct ggcccagcgc gttgatcatg gaggcgaaca ccacgtagcc cagcgaggtg
     1261 ccgaagcgct gcagcatatt gggcagcgcc accttcaggc aggggcgaag cacccggccg
     1321 tcgggccgca ggctgcagcc cttgggcgac accataggat gcctccacag caccgccgta
     1381 atcagcacgc cgccgaccgt gtaggaaatg gcgctggcga tggccgcgcc ctccacgccc
     1441 atccccgcgc cgtatacgct gacggtgtgg gaaaacaggc tgatctcccg ggtggggtag
     1501 atcagcagga agttcagcac gatgttgatg aggttcatca atacgcccac ccgcatgggg
     1561 gttttggtgt cgccagcagc ccgcagcacc gtgccgaaga ggatgctggc cgtgcggaag
     1621 agcatgggca cgtacagaat gaagaaatac cggtgggtca gctcccggat ggcgggatcc
     1681 acctgcatcc ataccggcac catggggctc aggctcaggg tcagcgcggt aaagagcgcg
     1741 cccacgatgc aggccaccat ggcggcctgc gccgaggcct gatgggcctc ctgcttccgg
     1801 cccgcgccgc aggctttggc aatgtaagcc agaaacccga cgcccagcgc ggagatggag
     1861 ctgcccacca tccagcctac ggtgctggtg gcccctacgg cggcggtggc ctccgtgccc
     1921 agcgccccca ccatggcggt gtcgatgtac tgcaccagcg tctgcatcag ctcttccagc
     1981 atggtaggcc atgccagagc gaaaatcacg ggcagcatgg cccagcgctc cttcgcgcgg
     2041 ttctttgact gattcaaggg aacgttcctc cgaaatcatt atcgcttcag ccagacagtc
     2101 tcgttgacga tgggggtgta gctctgaatg agcttgacca ccttcgcgcc cacgttcacg
     2161 tcctcgtaat cca
//
LOCUS       DAWAEA010000239         2173 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_6978_length_2173_cov_2.066572, whole genome shotgun sequence.
ACCESSION   DAWAEA010000239 DAWAEA010000000
VERSION     DAWAEA010000239.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6978_length_2173_cov_2.066572"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    558..567
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          429 a          679 c          632 g          423 t
ORIGIN      
        1 cccggaacac attgcggttg atgcgctgca gcacatggct ccgcccttcg gattcaaaca
       61 ggaaggtatc gttgatatgg ccgttgccaa ggggaacagg gtctttgtcg agcccggcgg
      121 ggcagaactc ccgcgccagc gcgcgcagct gttcgttgga atagtccatg atgtttctcc
      181 actcctgatt catcagaaat cggcgctcgc ccgcgcccga acagattcag tattatatta
      241 tatcatgaaa cgactttggg aacaagtacg gaggttttgc cccccggcag agcaaaaaat
      301 cgcctgcacg tccgcaaaag cagacaggca gacacattgg ggctttaacc aagaatcagc
      361 cgttcggcct cttcatagct ggccgcgacc gcattggctc catggttttc cggggcggga
      421 cgaccgttgg tgaacagaat gctgaacacc ccggcggcgt tggctgcagg gatatccgcc
      481 gccgcgctgt ccccgatcat cacggcgttt tcgggtgcga cgcccatgcg gcgcagcgcc
      541 gcgtggacga tggcgggnnn nnnnnnngct gaatggccgc cacgccgttg gtgaccagac
      601 agatgggcat tttttcggcc acccgggcgc aaaaagcctc cgccccgggc agaaggatgc
      661 tctggcagct gagccggtcc cggaaataaa ggtgcatctc ctccggggag cgccgcaggc
      721 ccaccgccgc aagaaagttg gccatgcggg ccacctgcag ctccggcagg gtcgtcctgc
      781 cctgctccag cagcgcccac tgctcccgat tgatccggtg ataggtctga aaattttctt
      841 cccgcacggg aatgtcaaaa tgggtaaaca gctcagtcag ggcgttctgt tcgcccgcgt
      901 caaaatcaaa cagcgtgccg tccgcgtcgg ccagcagcag ctcgtaagcc atcttccttc
      961 atcctttccg tttgccgccg cccttggggg cgcttcggcg tttcaatgcc ttgggggacg
     1021 cgacagaata accgaagaag cccagcggct tgcccacggc gcagtattta ccgtggacat
     1081 aggccaccaa ctccatatcc tcttcccgga tggagccgga ctcggtaaag tattcgtccg
     1141 ccacatagac ctgttccacc tccgccagaa acagatcgtg gctgcccagc tcctgcacgc
     1201 ttttcacccg gcagcacaga taggcgggcg cttccgccac agcgggggcg tgagtaagcg
     1261 gcggcgcagg gatgggggtc agcccgcagg cctcgaattt gtttacatcc cggccgcttt
     1321 tgaccccgca gaagtccacg gcccggcaca ggctgcgccc ggtcaggttc agggtaaatt
     1381 ccccggtgcg ccggatcaac tcgtggctca accggctttt gcggatgctg acagagacca
     1441 tgggtggcct ggtgcacacc acgcccgtcc atgccacggt gatcaaattg gcctcatagc
     1501 cgttttccgg gtcggcgcag cccagcatca ccgccgggat gggacttaga tacgtcgtcg
     1561 ggccaatagg ccggaaagtt tttccagtgc cgggcttcac gcgatctccc ccttgtgtct
     1621 ggtttccaaa aagtgtacaa tgaatcagtt cctctgtcaa cgctcgctca aaggagggtc
     1681 ctcccttgcc gaaatatcat tatcgctatc gaaaacccca gcggcggcat ttccccgtcc
     1741 ggctggccct ggtgctgctg gcgctgttgg cgctgtgtat ctatcccttt atcgaggcac
     1801 ggacgctgac ggtagacgag cacacggtgc aggtggccaa cctgcccgcc aatctaaaaa
     1861 acttgaagat cgtctacgcc acggacatcc atcagggccg ctggttctcc caaagccggg
     1921 tcaacgagct ggtcaagacg atcaacgggc tcagtgccga tctggtcatt ctgggcggcg
     1981 actatgcaga ggacagcgac ggggccatcg aatttttcga gaacctgcct tccatttccg
     2041 cccggatcgg cgtatacgcg gtgctgggca accacgaccg caccgagccg gaaagcaatc
     2101 tttccgtgct catcggcacc atcaagggcc ggggcatcat cccgctggtg aacaacgtgg
     2161 cggcggtcaa gcg
//
LOCUS       DAWAEA010000240         2148 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7059_length_2148_cov_2.042045, whole genome shotgun sequence.
ACCESSION   DAWAEA010000240 DAWAEA010000000
VERSION     DAWAEA010000240.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7059_length_2148_cov_2.042045"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          452 a          724 c          581 g          391 t
ORIGIN      
        1 ctggaacagg ttgaaacgga tgccctgctg cgccgcgtcg tcgccttcga tggtcacatc
       61 gcaggtagac cagcgctgct tccaggcttc tacgtgctcc ttcttcaggt cttcatagcc
      121 cgtttcccgg gcgcggctgg ccgccttcac cgccatggtc accagcaggt tttcctcgtg
      181 atcccggtcg gtgaagcaca gggcaaactt ttccagagaa acagtttccc cggcggcggc
      241 cttgcccgcg taagtcagct ccacgtagcc ttcgtccagc ttgcgctcct tcagctccag
      301 ctgatcggac cagcagctca ggcccgcagc cacggcgaag cgcggtgcgc cgaagggatt
      361 ctccttcgtc ttggtcagaa gggccagtgc gtcctcggtc tgctcctcct gcagcatatc
      421 ccagaaggtc tcgtcatagt tgctgtccag attgcgcaca ttcgcgtcca gatagggctt
      481 catgacgatg tcggcttcaa actcgggcgt gatggaatag ttgaccgcca gcagctcctt
      541 ctgtgccagc gaaacaaagc gttccgcctt tacttcgatc ttgccgttgg gcgtccacag
      601 ggtgaaatgg cgggaaaaaa tgcccgtctg catatccagt tcccgacgaa agtccagcac
      661 atccgccttg gccagatcca gcggcttgcc gttgatctcc acatggatgc cgttcaggcg
      721 cacggcgttg atgaccttgc cgaagtactg gggatagccg ttcttccacc agcccacgcg
      781 ggtcttgtcc gggaaccaca cgccgccgat ataggtgccc agatgggtat cgccgctgta
      841 gtcttcctca aaattgccgc gcattcccat atagccgttc ccagtggagg tcaggctttc
      901 agaaagacgc attttgtcag gctcaaagcc cgtttcaatg actttccagg gatggatctc
      961 gtatgccatg aatccgttct cctttcaggc gcaatcgttt gtattgcaga attatggtaa
     1021 acgattcagg cgcgattgtc aagcgcgccg gggacttcgg ccggaaaaaa gcatgggcag
     1081 aaacaccagg cccagacacc caagacccac cggcgtatac aggccgtcca caatgcccgc
     1141 aaacccaacg gaggaaacag ccagcggaag ccccatggtc agcgcggcgg aaagcggcgg
     1201 ctttacgccc cggctgtcca gatcgtcccg aagggcatag agtaccgccg tcagagtggt
     1261 caacaccgcc agatacaaca gcgccacgca gacgacatac cccgtccgcc ggaagctgcg
     1321 gaacagccga acgatcggga aagcctcctg ctgcagaccg ggaaaacgca ggtacagcgc
     1381 gttggccccg aacaaaagcc ccgtcagcac aaggccggtc atgagggccc ggcgatcaca
     1441 gcagcggcat ccgcgcgccc ccttttcgac ccggcaggtc acgccgatgg cgaccgtcat
     1501 gttcatgctg ccgtaggcca ccgcccgaat ggccgcgaag atcagcccgc ccggcggggt
     1561 ctgctcctgc acgacgcttt cccccatgcc cggccggcgc agcagcacca gcatcacgcc
     1621 gatgaaaaac accgtcagaa acccgctgac cgccgacagc gggccaacgc cccgaaagcc
     1681 cagcatccac gccagaatca gtgtgcccgc cgcgcccaat ggatatgccc actcggacgg
     1741 ccaaagcagc tgcaccagat gaccggcagc ggaaaccatc gccccgcctg tcaccagcat
     1801 cagcatggcg acgcatcccc gggccaaagc gcccagcttc gtatcggaaa agcatttcag
     1861 tccgcaccag cagccggcat tttcctgctg tgcgcgccgc atacacagtg cgcacagaaa
     1921 caccatcgtg accgccgcca gcaggatcag gccccaggac gcagccccat agcgggtgaa
     1981 aaagctgtag atctcccgtc ctgaaacaaa acccgcgccg atcaccgcgc ccacgcaggc
     2041 cagtacgctt cttcgatcct gatgattcat ggcagactcc cccttctttc ctttttccaa
     2101 ggtatgaggg gcgcgttttg ccttatgccc cgtcgctttc caaaattt
//
LOCUS       DAWAEA010000241         2146 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7075_length_2146_cov_1.516021, whole genome shotgun sequence.
ACCESSION   DAWAEA010000241 DAWAEA010000000
VERSION     DAWAEA010000241.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7075_length_2146_cov_1.516021"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    261..521
                     /estimated_length=261
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          368 a          609 c          519 g          389 t
ORIGIN      
        1 agcataacat ttgccctagg acaggaattg ggagttgatc agaaaagata ccagaagttg
       61 caagacagct ttgaactggt gtatatttca ttgtcatttt cgctgatact tgtagcctac
      121 atatatatcc tccctttttt gcagatctat acagaaggga taagtgatat cgactatatt
      181 gatcggattt tgcctgccct ttttgtagtg attgcgctct tggaaaatag taggctatct
      241 tctgcgaaag caattcacgt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcctgtggg cggcgatcac
      541 ctgcgtggtt gtgatcccgt tgttcttcac agtttcatcg atgtttgacc gagaaagcta
      601 ccactatgca aagtctatca ttgccccata tatacagcga atcttccgcc gctctaacca
      661 gcagtccgca gactaaaccc gcctatgctt cacttcattt acgcgcagcc cccgttactg
      721 atcattttcc tgatggccgc agcgcttgcg ctgtggggct gtcgcgctac aaaaattcat
      781 atgacgctcc actggaaccg ggggaacctc attctaagtg ttccgatggc gatagccata
      841 ctctatacga ccatacttag ccgctcggag gggaacacag gacttgtcct cacccccttc
      901 gccgccctcg cggccgcgcg gcagcagccg gagctttacc gggagatgct gatgaacgtc
      961 ttcctcttct tcccactggg gctgacactg tccaacgccc tgccgcgcac atggcaccgc
     1021 tggcggcggc ttgccctcac gacgctgacc ggctgcgtcc tcagcgcggg gatcgagtac
     1081 gcgcagtacc gctttgccct gggcatggcg gagacggacg acgtgatctg caacacgctg
     1141 ggggcgttcg tcggcgcgtc ctcgctgctg ctggcccacg ccatggaaaa acacaaggaa
     1201 aggcccacga ccatgaccct gactgccacc gaaacacaat ttctgcatat cgccaagact
     1261 gccgtctccg gcggggaact gcccacggag gccgtggact ggcctgccat gtttacgctg
     1321 gcaaaccagc agaagctgct gcccatcctc ttcgaggccg tccgcaagac gcccgctgca
     1381 ggggagaacg ccccactgtt cgcggccatc aagcagcagg tcatcggaca ggtgctgaac
     1441 cagaccgtgc gctcggcgga gttcgccgac ctctaccgca gcctccgggc cgccgggctg
     1501 caccctgtcg tggtgaaggg gcagctttgc agcaggctct accccctgcg ggaccaccgc
     1561 atcagcgcgg acgacgacct gttcatcccg gagggcgagt ttttcgcctg ccaccaggcg
     1621 ctgctggcaa acggcctgac caccgacacg cctgcggacg agttgcccac ggcggacgag
     1681 gtgtcctaca caaaaaaaga cagcccgctt tacatcgagc tgcaccgcca cctgttcgac
     1741 tccgcccagg acgcccatga cgagctgaac cacttcttcg cgggcatcgc ccccgtggag
     1801 gtggacggct tcctgaccat gccgccccat aagcacctgc tgtacctgat tctgcacgcc
     1861 tacaagcact ttgtgtacag cggcatcggc ctgcggcagt tctgcgacat cggcctctgg
     1921 gcgcgggcgt accacgatcg gatcgactgg cagcgcctgc atgaccagtg cgccggggtc
     1981 cacgcggcga ccttcgccgc cgcggccttc cggatcgccc gggcgtatct ggacatcgac
     2041 tttgacctgc ccgggctgtg ggacggcgat gtagacgtgg agccgctgct gcacgacacc
     2101 ctttgcggcg gcgtgtacgg ctccaacagc tacacccgcc tgcact
//
LOCUS       DAWAEA010000242         2145 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7076_length_2145_cov_1.898565, whole genome shotgun sequence.
ACCESSION   DAWAEA010000242 DAWAEA010000000
VERSION     DAWAEA010000242.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7076_length_2145_cov_1.898565"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          406 a          639 c          730 g          370 t
ORIGIN      
        1 aaatgatccg ggcggcggaa aaactgggct tcgtcagcct tgggttttcc ggccatgcca
       61 gtcagggctt cgacttggaa tactccatga cggcagagac tcagcgggcg tatttcgaga
      121 ccgcgcgggc gttgggctct gcggcgaagc tgcacgtctg ggccgggctg gagcaggata
      181 ccttcgcccc ggacgcgctg aaagcggaga accgccgggc ggcggattat gtgatcggct
      241 ccgcgcacta cgtcagtctg gattttcacg gcagaacggt ggcagtggac ggcgaaccgg
      301 cgctccttcg gcagtatatc gaagaaaaat gcggggaaga cgcgcttgaa ctggcgcggc
      361 agtacttcga tgttcacgtt tccgggctgc ttcgggatcg gccggacatc atcggccatt
      421 tcgatctggt gcggaaatac gcgctgtcgg agagcctttt cgacgaaacc agcccggaat
      481 accgccgcct ggcgctggac gcgctggaaa gagcgttccc ctgcggcggc gtgctggagg
      541 tgaacaccgg ggccattgcc cgaggctacc gggacgaccc ctatcctacg gcggagctgc
      601 tgggcgcatg gcgggagatg ggcggacgca cgaccatcac cagcgactgt cacgacgcgg
      661 ccaagctgga ctgcgccttt gaacgggccg cggagctgct tcggcagacg ggttatcgaa
      721 ccgtgctgcg gctgggcacg ggcgagacgc tctggcagga agaaaatctt tgaacggatt
      781 ttcagtgaat aggaggaaag aaccatggca gattttgccg caatggtagg cgcgcagcgg
      841 aagtattttg actcacaggc cacgaagcct tacgaatttc gccgggagca gctgaaaaaa
      901 ctgcaaggtt ggatgaccaa aaacgagcag gccattctgg acgcgctgca tgaggatctg
      961 ggcaagagcg actacgaggg ctacctgacc gaggtggcca tggtcaagca ggaactgacg
     1021 gacgcgctga agcatcttcg aggctggatg aagccgaaaa aggcgctgac tgccatgggc
     1081 cagctgccgg gcacctgccg cattctgtcc gagccctacg gagtggtgct catcatgtcc
     1141 ccgtggaact atcccttcca gctgacgctg gccccgctgg tgggggcgct ggcggcgggc
     1201 aactgcgcca tcgtgaagcc gtcggcctat tcgcccgcta cctccggcct cgttcgccgc
     1261 atggcggagg aactgttccc gccggagctg gtgaaggtgg tggagggcgg acggacggaa
     1321 aacgccgccc tgctggagga gcacttcgac tacatcttct tcaccggcag ccctgcggtg
     1381 ggccgtctgg tgatggaaaa ggcctcccgc cacctgaccc ccgtcagtct ggagctgggc
     1441 ggcaaaagcc cggtcatcgt ggaaaagagc gccgacgtcg ccctgaccgc ccggcggctg
     1501 gcttggggca agtgcctcaa cgcgggccag acctgcgtgg cgccggacta tgtgctggtg
     1561 gatcgcagcc gggagcagga gctgatcgag gctctgatcg gggaaattcg gcgaatgtat
     1621 accagcgcgc cgctggccag cgaggatctg cccaagatcg tcaaccagcg ccacttccag
     1681 cggctggtgg ggctgctttc cagcggctcg gtggccttcg gggcccagat cgacccggcg
     1741 gaacggaaga tctcgcccac cattctgacg gacgtggagt gggacagccc cgtgatgcag
     1801 gaggaaatct tcgggccgat tttgcccatt ctgacctatc ggaatctgga cgaggccatc
     1861 cagcgcatcc gccagcggcc ccggcctctg gccctgtacc ttttcacccg ggacaaggcc
     1921 gtggaggaca aggtgctggg ctccgtcagc ttcggcggcg gctgcgtcaa cgatacggtg
     1981 ctgcatctgg ccacgtccca catgcccttc ggcggcgtgg gggagagcgg catggggggc
     2041 tatcacggcc cgtggagctt ccgcaccttc tcccacgaaa agagcatttt gaagcgctgg
     2101 gccaagcccg atatcgccct gcgctacgcg cccttcaggg gcaaa
//
LOCUS       DAWAEA010000243         2128 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7129_length_2128_cov_1.440425, whole genome shotgun sequence.
ACCESSION   DAWAEA010000243 DAWAEA010000000
VERSION     DAWAEA010000243.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7129_length_2128_cov_1.440425"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    923..1017
                     /estimated_length=95
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1435..1585
                     /estimated_length=151
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          389 a          542 c          549 g          402 t
ORIGIN      
        1 gaatcgcatc ggtcttcttc ttgtcgatga agtcgctgac aatcttggcg gggctcacgg
       61 ggtcgctcat gccaaaggca aaagcagagg ggccgttgag cacatcgtcc agaccttcga
      121 tgttcagctc gtgcagggca cggcgaacca gcgtgttctt cagaacgcag tattccacgt
      181 tgttctgacg gaactgagcg cgcagggcgg taacgtcttc cacggacagg ccgctgaagc
      241 tgaccacgat gacgctctgg gcatcctgaa acttcttcac gatgtcgctg acgacttgct
      301 ttttgatctc gagattcttg ctcatttgag ttgcacctcc tttttgaaat ggtcgattcc
      361 aaaaagaaaa cccttgcagg cgcaagggcg aacatgtcgc aggggatttc tcccgcctta
      421 cacctcggcg ggcgctgctt acgcttctta tgagggacag gccctcaccc gctgtcttcg
      481 gcataggttt ccaaagaatc gtttatgatt atacggattt ttcccctgtt gtcaagcgtt
      541 ttttccgctt tcttccgcgt tttcgaaggg ctccggctca tttttcggca cttggcagaa
      601 aggctcggaa atatgtttgt taaagaaatg gacgaagggc ccaaggcaaa gggcgcaggc
      661 cagcgttccc cagcccacga tgccgcccag caggaacgcc agcagcgtga acgtgccatc
      721 caccgcgatg cggcaccaga aatagggaat tttgggcagc cggtcatcca gcgtcagggc
      781 aaggctgtca tagggggaag cgcccaactc cgaggactgg tacagggaca cgcccagact
      841 gacgaagggc agcgccagca gcatgatgat aaagcggggc aggaaagctt ccgggaaggt
      901 aaagagctgc ttgagcagcc acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngca
     1021 tcggagccag cgccatgccg accttgttcg ccagagccag cacaaaggcg gtgctggggt
     1081 cgttgcccaa cagagacatc cgaaaaatcg atacgctgat cccaatcaga aaattgccca
     1141 gaaacaccgc gatcagccgc cgcgcagtat accgtttcac gcttcgttcc atccttcatt
     1201 cacaagatta ggggaaagcc caaagagagc gccgcgctcc tgtcggaagg cgcggcgctc
     1261 cctgttcagc ttgaacagga gacggcgggt cagtccaccg ccttggtttc gatttcgtgc
     1321 ttcagcttgg ccaccagatc gtcgatggac atgttgccaa tggtctcgcc cttgcgggta
     1381 cgcacgttga ccacgttctg ctctacttcc ttgtcgccca gcaccagcat gtagnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnatgaa ccgctcgatg gagcccagca ccacgcggtg
     1621 gatcatcacg ggacggtgct tttcgccgtc cgcgccggta taggtcaggt caaagcgctc
     1681 gggcaggttc atatccagct ggatggtgcc gcactgccat gtacggccca ggcagtcctg
     1741 caggtggaag tcgatcttgg ggccgtagaa cgcgccgtcg ccctcgttga cctcgtagtc
     1801 catgtgcagc tgctcaagag cggtgcgcag gcttacgctg gtaaaccagc atgccgccgg
     1861 ggcagttcat gggcttgatg gcgaagtcca tgtcgtcgat cttggtggtg tacatgttct
     1921 ccttgtagtg atcccagtgg ccggagcgct cccacagggc ccggttgagc atgatgggcg
     1981 tcttgatctc ctcatagccc gcttcccggt gaatctcgcg ccagtagtct tccagacagt
     2041 tgcgcaggat cattcccttg gggtagaaga aggggaagcc ggggccctcg tcccggatgt
     2101 cgaacagatc cagctcgcgg cccagctt
//
LOCUS       DAWAEA010000244         2127 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7133_length_2127_cov_1.211873, whole genome shotgun sequence.
ACCESSION   DAWAEA010000244 DAWAEA010000000
VERSION     DAWAEA010000244.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7133_length_2127_cov_1.211873"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    407..416
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1205..1253
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          431 a          526 c          630 g          481 t
ORIGIN      
        1 ccgaagaacg ctttttcgcg ccttcggtct ttgcagacgg tcacacgcct gccgcgtccg
       61 tctgagcctc ttcgggaacc gggtcttcca gccccagcag tgtgctcagc gtcagacatt
      121 cgaagccctt gtccagcaga ttggggatca gcttggtcag gcagcgcacg tccttggcgt
      181 tggcatggaa aagcagaatg ctgccgttct gcgtgttctt cagcgctttt ttcgcatcgg
      241 tggtatccac gtcccagcgg acggcatgga cataacccgc cttatagatg gactctacca
      301 cccacatatc gctctttttg cccttcttgg aggacagatt gccgtagggc gggcgcatga
      361 gctgcatggg gtagtggtag cccagcacct tgtccagctg ctcctgnnnn nnnnnngcct
      421 tgcccaccac gaaaaacgta acgggaatgt tgtattcttc gcacagatcc agaatttcgg
      481 tcacatgttc ccggctgtag cagtcgtcca cggacagggc gatctgcgga acgtcacggt
      541 cgccgtggaa aacggtaaat ccctcaggaa cggtcgtccc ggcgctgccg agcgcggggg
      601 aaaggagcat cgccagaaga agaagaacgg aaagaaaacg ccgggatacg ctgcgcatgg
      661 gattgcctcc tgcatcgggg aaagccccgt tttttcggtc gtggttctca tcctatcaaa
      721 cgaaggaaaa ggagaaaaac ttccagctta tgtcggcttt ttggggaaaa agccaacatg
      781 aaatgatacc atggacgaat cggaaataca aggacttgta taattttctt ttgccgcgcc
      841 agactgtagc cgtaaggggg aatgaaaatg ctgggttttt tgttcgcggt gatcgccggg
      901 gcggccatga gcgtacaggg cgtgatgaat acccgcctgc aggagaatat cggcctgtgg
      961 gaggccaacg cctttgtgca gggcacagcc tttgtgctgt cgctggcggt gatgtggatc
     1021 ttcggcaagg agcacctgcg cagtttggcg gaggccccat ggtggaacct gctgggcggc
     1081 gcactgggac tggtcattac cgttacggtg atgctgtcca tcaagggtct gaacccgacc
     1141 attgccattt cgacgatttt gatcgcccaa ctgctggtgg cggcggcgat cgactatttc
     1201 ggctnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngccgggg
     1261 tggggctgat gctggcgggc gtggtgctga tgaagtggac gaagtgaaaa gcacagaaaa
     1321 aagtcatgct ctttgacaaa gcatgacttt ttcaaacggc ttgtttgtta ttctgcggtc
     1381 gtatcggtgg cgggggtgtt gttcagaaag gcattggtca gggtgttgag ctcgttgatt
     1441 gcgtcggtga gcttgctgag cgcgtcgttg atcttctgcc cgctggccag acaggaatcg
     1501 tagttgtcga tgatggcgta gatgttgtcg aaggtgctga cgtcgtagag ccgtccgacc
     1561 tcgccgtaca tgctgacgtt cagatcgttg accacttcga tgccgtggac gtactgccgt
     1621 acccgcgcca gcacgctgga tgtgctggta gcgcccagac ggctcagatt gttgacactg
     1681 gaaagggcgt gctgcatctc gctgcgaatt ttggcgttgc gctgggtctc gaagctggcg
     1741 gaatccagcc cgcccacgcc cgtaaagcgc aggatcagga aagccgtcag aagcaccagc
     1801 aggatcacca gaatgggctt gtaacggttg gagcgcatca cgttttgata gctgctgcgc
     1861 tgataacgca tcatgggatg ttcctcctcc ttctaaaaaa gcctttcggg aagggagata
     1921 ccatcagcag tatagcacaa gtggattgaa aatgcaaaaa ataccccctt tttcgtctga
     1981 aaagggagca ttacgcataa aaacaggatt attttccttc gatgtcgctg gtgcagtggg
     2041 ggcagcgggt cgcgtccttt tccacgacgg acttgcagta ggggcacttg cgggcggctt
     2101 ccttcacggc ttcttccttg tgcagca
//
LOCUS       DAWAEA010000245         2120 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7154_length_2120_cov_1.645521, whole genome shotgun sequence.
ACCESSION   DAWAEA010000245 DAWAEA010000000
VERSION     DAWAEA010000245.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7154_length_2120_cov_1.645521"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1333..1342
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1637..1788
                     /estimated_length=152
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          350 a          588 c          582 g          438 t
ORIGIN      
        1 gggcgtggag accaccttgg gcgaggtgcg gtagaggacg gttccgtccg gggaaacggc
       61 ctccttcagc agataaggcc gcatgagccg tccgccgttg agaacggagc aggcggcggt
      121 cagcagctga atgggcgtta ccgccacgct ctggccgaag ccgatccgcg ccagatccac
      181 attcttgacg gaattcacgg gtattagtat accactttcc tccccctgca agtcaatatt
      241 cgttttcgac cccaggccaa aagcgtgcag gtactggtaa aaccgctggg cccccatgcg
      301 cagggccagt tccacaaaga ccgggttgca gctgttctgc agggcctgcg ccatggtctc
      361 ggccttgtgg ggactgcccc agcacttgat ggtgtcccca tccaccttga tcttgccgga
      421 gcagtagaag ccgtcctccg gcgtggtcac gccgctgtcc agcgcagcgg cgggcgtcag
      481 aatcttgaag gtggagccgg gctcgtagga atcggagatc aggcggatgc gcatcaggct
      541 ttgcagcgcg tccagctgtt cccggggcgg gtcgttgggg tcgtaatccg gcttggagca
      601 catggccagc actgcgccgg tatacacgtc catcacaagg gcgtgaacgg cctgtgcctt
      661 gttgacctcg tagcactccc gcatggcctt ttccacgatt tcctgaatgt tcgcgtcgat
      721 ggtcagacga atggtgctgc catcctgcgg ctcaatgtag agattgccgc tgtcgtagat
      781 agggcgggct ttgccgtcca cactgcgcat actgcggccc gcttcgcctc gaagcagcgt
      841 ttcgtactgc tgttccagcc cggactggcc cacgccgtcc acgttcacca agcccaaagt
      901 ctgggtcaga aacgcgcccc gcaggtagac gcgacggaca tcctcgtcaa acaaaagtgc
      961 ggatgcggcc gcagcggtct ccgcatcctt actttgcttt agttgacgca gctggtctgc
     1021 cgtttcccgg gagacctgcc gtttgagcgt tacggaggat tttcctcgct gagaggcctt
     1081 ttccgccact ttttcctcgg aaaggcccaa aatcgggctg atggcccggg caaatgcctg
     1141 cacgtcctgt acctttccgg gttccacact gacgatatag gccgtggcgc tcatcaccag
     1201 cgtttgaccg ttggtatcca gaatattgcc ccggcgggca tagaccgttc cttcccgcgt
     1261 ccactggcgc acgccccggc tggtcagttc ggcggagcgt gcggtggtca gataggcgat
     1321 ccggcacacg agnnnnnnnn nnaagaaaaa tcagcaggac cagaagggtc agaactcttt
     1381 tccgaatgtg cagctccgca ggcatgatac ttcccccatt ttacggtttc ggtgctaatg
     1441 ggatgtatgt tgccttttag gcggatagaa ccctttcgac gagcagtctg ttttttcggc
     1501 gttagttgcc ggaatccgca gagacgtgga tcgttccggc gggaacgtcg gcttcgttcg
     1561 ccacgcttgt attgacactt tcctccgttt cagcggaagc ggtcacagcg gcggggcggg
     1621 tgtccacagc caccagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc gctctttttc
     1801 cagcatcagg gcctgtgtgc ggcgacggcg ctgacgatcc ggctgaagcc ggcccgttct
     1861 caggtcgata tcgctggcgg gcagatatac gctgcttttc acctgaaaac gctcggtggg
     1921 cacatactgt tccgcgccgc gctgggcaaa gctgtcgtaa gtgtacacat attgttccat
     1981 ggttgcttct cctttccgta ttcgttacgg aaaaccttta ttcttccatc atattgccga
     2041 tttcgtccag cagggcctcc cggtgctccc ggaagaagag atcctcgtcg tcggccgtct
     2101 tgcggtccac aatgcggatg
//
LOCUS       DAWAEA010000246         2106 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7210_length_2106_cov_1.900536, whole genome shotgun sequence.
ACCESSION   DAWAEA010000246 DAWAEA010000000
VERSION     DAWAEA010000246.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7210_length_2106_cov_1.900536"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          353 a          657 c          649 g          447 t
ORIGIN      
        1 tgtcggtata gggcggattt tcgatggtgc cgatctgata gaaaaatgcg tccagcttcc
       61 gttcggcggt gacggcggga tccagattcc gcagggcgcc tgcggcagcg ttccgggcgt
      121 ttttcagcgg ctcgtcatgg gtcttgttgt acgcttccag cacgctcagg cgcatgatgc
      181 actcgccctg aatttccaga aggccctgcc acggaatgga cagaggcacg cagcggatgg
      241 ttctggcctg cggcagaatg gcctctccca ccacgccgtt gccgcgggtc gcggcctgaa
      301 tcagctggcc gtgatcgtag gtcaggttga tggtcaggcc gtccagcttg tattccaccg
      361 cgtattgcag cggcggcaga ggttcttccc ggccgtcgga cagctgagcg tggagcttct
      421 ccgtgcgggc gatccaatcc cgcagttctt ccttgctctg cactttgccc atactccaca
      481 ggcggctgag gtgcttatgc tgctcaaaat ttttcagcgg ctccgcgccc acccgatggg
      541 aaggagagtc gggcagcacc atgaccgact ctttctccat ctgacgaagc tgattgtatt
      601 tttgatccca ctccgcatcg ctcatggggc tgtcgccgcg ggtgtaaaag agaacggacg
      661 cccggttaag ctcgtccacc agagcgcgca tttcaagctg cgtcatgtca gtcgaccacc
      721 ttcacaatgg gggcgatgga cagggcgaat tccttgctgc cgtaagcggt aaagctgatg
      781 acgatgcggg cgtcattgcc gctgccgcga atttccagca cattgccctc gccgaattta
      841 cggtgcatga cccggtcgcc cacctcaaag atcgcgggca ccgcgtcctt gggcacgctg
      901 gccgccgccg agggggtgaa gcccttctgc acgccgggaa cgcccagcgc gctgcggcct
      961 gcgcccagcc cggagcccat ggcatggcgg ggagcccgct tgggcgcggg atgcgagtac
     1021 gcgtccatca cgccggggaa gctcttttcc cgctggctga tccactggtc gtccagcagc
     1081 cgttcgggaa tttctttcag gaagcggctg ggcgcgttgt aattgacctg attatacagc
     1141 atccgctggg ccgcgtagct gatgaacaac atctcccggg cccgggtgat ggccacataa
     1201 cacaggcggc gttcctcctc cagacgggta tcgtcgtgca ggctgcggcc gttggggaag
     1261 atgccttcct ccagccccac catgaacacg atgggaaatt ccagaccctt ggcgctgtgg
     1321 atggtcatca gcgtcacgtt ggaggatttc aggtcggcgt tgtccagatc ggtcaccagc
     1381 gcgatgtttt ccagatagtc ctccaaagaa ggattatccg cggccttttc gtattcggcc
     1441 accgcgccca gaaactcctg tacgttttcc atccgggcct tgccctcgtc ggacaggtcg
     1501 cgctcataca tttccttcag gccggtgcgt tccagcacgt gctccacaaa ttcgctgacg
     1561 cccatgtcct ctcgggtcag caccagctcg ttcatcagct cgccaaagtc gctgacacac
     1621 ttgcggggcc gggcggacag gctttcggga atatccacca gcgcgctgta caggggcatg
     1681 tccttctcgg cggcgtagcg gctcagctct gccacggtgc tgtcgccgat ggagcgcttg
     1741 ggctggttga tgatgcgctt caggctggcg tcatcggagg gattgacgat ggcccgcaga
     1801 taggccacga tgtccttgac ctccttgcgg tcatagaagc gcaggccgcc gtaaatacgg
     1861 tagggaatgc ccgcccgcac cagcatttct tccagcacgc ggctctgggc gttggagcgg
     1921 tacaatacgg ccatatcgct gtattcccga tggcggcggt gctgctgggc gatgcggtcg
     1981 cagacccacg ccgcttcttc ccgctcgtct ccggcggcga acagcttaat gggttcgccc
     2041 gcgggcagct ccgtccacag ggccttttcc atgcggcccg cgttatgggc gatgacctga
     2101 ttggcc
//
LOCUS       DAWAEA010000247         2105 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7212_length_2105_cov_2.137073, whole genome shotgun sequence.
ACCESSION   DAWAEA010000247 DAWAEA010000000
VERSION     DAWAEA010000247.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7212_length_2105_cov_2.137073"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1341..1350
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          350 a          625 c          557 g          563 t
ORIGIN      
        1 acttcgttga tggtgtccat caggctctgg ttggtctgct tcagggtctc cagatcgatg
       61 atgccccgtt cggcctcctg tgcggtctgg atggtgccag tcttgagcgc ctcggcgttc
      121 ttgcgcagca gttcgttggt catgttggtc acagccgtct gggccttcag cgcctcctga
      181 ctgcggtgca ttcccagtgc gatcacgatc tggcttttcc aaagcggcag cgtgttggaa
      241 agggtgctct ggatccgttc caccagcaga ctgtcattat tctgcaaaag ccggatctgg
      301 ggagccatct gcagggccac ctgccgggtc agcttcagat cgtgaagctt tttttcgaag
      361 cggtcgcagt tggcggcaag gtcgttggcc cgctgagcgt ccatcgcgtc gccggtctcg
      421 gcggccttct ttttcagctc ttccagctcg ccctctcgaa cttccttcaa tttctgctca
      481 ccggcgatga tatacatcgt cagttccttg aaatactggg tgttcatatc atacaggtga
      541 ttgaacatgc tcacgtcctt gagcagctgc acctgatgat tttccagctg gctggcgatg
      601 gtgtccacat tcgcgtccac cttatcgtac ttggtctgca tatcactgac ggccttctgg
      661 gcgttccaga acaggccctt gatgccgctg ggcttttcgg cggcggcgct gaagcccttg
      721 atctcgttga ccagattcac cagcatatcg cccacttcgc cggtgtcctt ggtgcggatg
      781 gtggccagcg cgctgtcgct gaaatcggcg atcttcttct gagcgtccgc gccgtaaagc
      841 agcacgtggt cggagttatt cacgtcgatc tttttgacaa actcctcgat gctcttttta
      901 tcttcttcgg tcagcatggt ggtgtccatc tgcttgaccg cttcgctggc ggcctgcgcc
      961 gctgcggtgg cgcttggggc tgcctgcgcc atggcttcca gctgctgagt cgtttcctcc
     1021 agctgcactt tttccttggc gaccgacgaa ggatcgaggg tcaaaacagg ggtctgctgg
     1081 gtctcattgt tctgctgatg aatttcactc atgggaaaat tcctccttca atcgctctat
     1141 cgccggtttg atgggactca ttcttctttt ttggcctgtg ccgcggcctg accgtcatac
     1201 acggggtaga ccccgccgga catcgtagga gcctgaggca tatgcttccg tgcctgttcc
     1261 tctgcgccgc tgacggcctg ctgcacgcta tgatccgtgt gaagggtcgg cgcgctttgc
     1321 cgggtctgcg cctcctgcgc nnnnnnnnnn tttggcctgt tcggcggctt tttccagttc
     1381 gctttccgtc aggccgtccc gcttgagcat ctgttccagc acgtcgatat cggtggcgat
     1441 gtccagcata tcgtccttat aaagattctt aagcattttc tgacagccgg tgatcaccac
     1501 gcccagcgcg tcgtcaatgc ggtgctgtgc ttccttcgcc tcagccatgg gcaggttgcg
     1561 ctcgtccagc ttgcgatagc tcttgaccat cttgagcgta gtgggcaggt aatagtccat
     1621 gaacttgcga atctgcgggg cgcgggcagg attttcatac acggcctgaa aaatcgccgc
     1681 gcagttttcc tccagatcgt ccagcttggc cgacaaagac tcgtcgggga tcagatcgtt
     1741 ttccttccgg atttccgcga tcatttcccg gccctggttc aacagcgctg acaaacgcac
     1801 tcaccgccag ctgtaccgcc agactgaaca gaccgggaat cttaaaaata gaactgagca
     1861 gcacgaacgt cacgccgaaa gcaaccgcac ccgcgccgtt gccgctcttt tttttctttc
     1921 ctttactcat tgcaggctcc ttcatccatc ctctatctca gccggggcgt tttcgcccgg
     1981 caacttggcc gacgcattgg attataccat atttcccctc atctgaaaag aagaacccgc
     2041 tcgaaacaac ctttgccctt cctaaaagtt ttctgaaaat ttcatgagca caaggggcag
     2101 tgccc
//
LOCUS       DAWAEA010000248         2070 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7360_length_2070_cov_1.641191, whole genome shotgun sequence.
ACCESSION   DAWAEA010000248 DAWAEA010000000
VERSION     DAWAEA010000248.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7360_length_2070_cov_1.641191"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          387 a          649 c          599 g          435 t
ORIGIN      
        1 tccgtttttc ggaaccgtta caaacgctca ttgttttccg cgggctgttc atcgccccgg
       61 cggatctcgg tgactttcac cttccgcagc agcacgattt ggccgatccc cgcccccagc
      121 tggagcagca cacccagcag gaacgaaacc tcgtctgcaa tcgtttgatg gacaatacac
      181 gcaatgatgc tgccgatggc agccatccct gcgcacaccg tcaatcccca cgcgctgtga
      241 accatgcgca tccaggcatt gtccataccc ttgcgggtga aatcgccctt gtgcagcgcc
      301 cattcgacca gccccatcag gaaatagccg gtgggcagca gcccgcagac gaaggggagc
      361 agcgcataaa aggtctgcgc ggtacgccct ttgctgaacc cgcccaacac aaaagctccg
      421 gcggcaatca gcccgagcgc ggtcagcagg aagatcatct tccgcctttc gcgcccttcc
      481 agctcgatat ggtacatcga gccgatgtag tgataaaccc cttttgcatc cggcgcgtag
      541 tcggaattat accggccccg gccgaaacgg gtgcttttga cgtttttttt ctgactcatt
      601 gcagctttcc ggcgtcaaag ccttccagcc actccttggc cttggcggcc accttgcgca
      661 gcgcctccgg gccgaagggc gtctccagcc ccgccgcgtc ggtcagctgg cggaaggtgc
      721 gggttccggc gagcttcacc agatccatgt agcgcttcca cgccgcggcg tgatcctctt
      781 gcgtcagcgc ccagaactgc agcgcgaccg actgcgccag gcagtagtcg atgtagtaga
      841 aggggctttc gtagatgtgg tgctggcgct gccagccctt gccgtcgcca tagaagggaa
      901 tgtcgtcccg gcgcatccag ggcatataga ccttcagcag ctcgcgccaa atattgtggc
      961 gctgctccgg ggtgtattcg ggatgcgcat acacctcatg ctggaagtga tcgaccatcg
     1021 tgccgtaggg gatgaaggtc agcgcggcgg acaggtgctt gtagcggaac ttgtcctgct
     1081 gatcgccgaa gaaatcccct gcccagggcc aggcgaagaa ctccatggac atggagtgga
     1141 tctcgcagct ctccagcgac ggccactggc attcgatggg aacgatgttg cgcgccatat
     1201 atcccgcaaa ggcgtgaccc gcctcgtgcg tgatgacctc gacatcgccc tgcgtgccgt
     1261 tgaagttgga gaagatgaag ggcaccttgt aatcgggcag gctcgtgcag tagccgccgc
     1321 cgaccttgcc cttcttggcc accacgtcca tcagttcgcc gtccagcatc acgtcgatga
     1381 actcggcggt ttcgggcgac agctcgtggt agaactttct gccgtttgcc aaaatgtcgt
     1441 ccgccgtgcc caccgggcgg gcgttgccgg agcggtagaa cagcgcttca tcggcaaagc
     1501 tcagcggata ctgaacgccc aggcgctcgg cctgcttttt gcagacctct tcggccacgg
     1561 gcacgagatt ctcccgcacc gaggcgcgga aggcttcgac gtccttctgg ccgtagcagt
     1621 tgcgctgcat tctaagatac ccgagagata tataattgtc aaatcccaat tttttcgcca
     1681 tacgggtgcg aatggcaacg agcttatcgt agatctcgtc cagcttttcg ccgttggcgg
     1741 tataccacgc gccctccgcg atccaggcgc tgcggcgctg ggtgtcgtcc ttgctctgct
     1801 tgaagggcgt catttgcgcc aaggtatagg tgctgccctc aaattcgatc tgtgccgagg
     1861 ccagcaggtt ggaatactcg atgcaaagca ggctttcctc ctgcatatcg ggcacgattt
     1921 ctggcgagaa actcctggcc tccaattcga ggttcaggaa catcacctcg ccgtacttct
     1981 tctcgaagtc cttgcggaag gggctggtca gcagcgcctg ctcccaggcg tgggtgcttt
     2041 cgcgcagcat ggggcgagcc ttggccagct
//
LOCUS       DAWAEA010000249         2041 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7471_length_2041_cov_4.192346, whole genome shotgun sequence.
ACCESSION   DAWAEA010000249 DAWAEA010000000
VERSION     DAWAEA010000249.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7471_length_2041_cov_4.192346"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          409 a          630 c          628 g          374 t
ORIGIN      
        1 ttaccgccgc tcggcgacag gcctcgcggc tcgccggtct ggtgtggttt cctgtcagtt
       61 ggcgtatgca gaccggcact cgacggaagg tctcgtggct tgcgctacag gctaacgtga
      121 tttgaaaatc actttggctt tttgccttgc aaaagcggcc accccacgcg gaagcatccc
      181 aattcgcact acttggcctt cgccctcacc caagtagcgg cggagcagag cggcgatagg
      241 cagcggaggg aaaaagcctt gccctttttg aaagaggaaa agagtgagca cgagcgcgca
      301 cgatagcatg agttagttcg ccgactaaat tgcgcgcgac attgcgaact ccctctttca
      361 aaaatgggca gtaaagggct ttttcccggc gggtgagcgc agcgaaccga ggtcgaggac
      421 agcccctgag ggcagtctgc ccacggaacg tggtcagact gttccggccc ttaggccgga
      481 attggatgcc ccgaataaat tcggggcgtc caaccgttcg gctcccgcag gccgaagccc
      541 gcgacgcgca gcggggcggg ccgcctctgg agtcgccgtg ccgcgtgctt tctttggtac
      601 tttctttcgc gccgaaagaa agtactgccc tcctcggggc ggcagaccca ccgtccgcgc
      661 cggagtcggc gcgtcaatcc cctccgcgga aaggggtatg cgccaatggg cgcaccaaag
      721 ggctttccgc tcgccctttg gaaaccttcg ggcaccgaag ctggcgagaa acccatagga
      781 cggcaagtcc caatccgcac tgtagtcggc gcattatgcc tccggtagga aattttttac
      841 ggcagagttg ttgctctccg acacaagcag ggggctttcc gctcgccccc tgcacccctt
      901 cggaccgtag tcgtcgaaat tttgacggaa tgaacagaaa actttcttcg ggggcaaacc
      961 atcggcgtgg cccgtatccc cctccctcta acccaatatc cctcagaaag gagccactcc
     1021 ctatgccgac agaagtctat gaaatcatag ctcagggcgc acggtactgg ttcctttttt
     1081 tgatggccct gatcgtgtgg cgcagcttcc gctggtatcg gaaggatcgc cgccagtata
     1141 agaaacgcct gcgcctgctg cccgacgcgg gctacgtagg cgaactggtc acgatccgcg
     1201 gcaatgccca gctgcccacc ggcactatcc tgcccgtgtc gtgggaaggt attatcggct
     1261 tcgggcgcgg ctgcgatatt ttcctgcctg ttccgggcgt ggcccccaag cattgctggt
     1321 tctcctatga tcgggaggac gggcttcagc tggaacccta ccatggccgc accgtgcagg
     1381 tgaacgacca gacccggacg ggccggggcg aaaacctgag tatgcatcac ggctcccgcc
     1441 tgtatgtggg ggacgtggag ctgcgtctgc ggatgttcgc gggctttgag accgccgcac
     1501 gggcccatgt gctgcgggac gagccgcagc ccggcggcgg gcaactggcg gcagcagctc
     1561 ccgtcatgac gcaggaacaa ttcctgcgtt ggcagcagca gtacatgatg caggccgctc
     1621 agcagcagac gatgcagcag caggcctatt tccagtggat gacccagcag tgggccgctc
     1681 aacaagccgc gatgcagcgg cagatgcagc aaaacgccgc ggcccagcca acaatgcagc
     1741 aggaagaacc cccggaggag ctttcttcgt ttgaaccgca gaaaaccccg gagatggagc
     1801 tgcctcaggc agagcctgat ttcgatatga ctgcgccgac cgtggagttc cataccggcg
     1861 aaaacttcta tccgccggag atgaccgatg cggaggaagc cgcatggccc tacgcgccat
     1921 ggccggagga tttgcagccc ccgtctgggg agggcttcgt ttctcccaat gcggacgacg
     1981 accagaccga cgcggccgca ccgcctaaga gcgcctacgt gggcgaggat gaggcgcaga
     2041 a
//
LOCUS       DAWAEA010000250         2034 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7498_length_2034_cov_2.424457, whole genome shotgun sequence.
ACCESSION   DAWAEA010000250 DAWAEA010000000
VERSION     DAWAEA010000250.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7498_length_2034_cov_2.424457"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          307 a          606 c          686 g          435 t
ORIGIN      
        1 cagtccgggg tgcagggctt tagccctgcc acggcggaaa acccccggcc aagccccatg
       61 tccccctacg cccctgcgcg caaggcgcgc agtgatgggt gcagggcttt agccctgcta
      121 tctgccggcg cgcttctggg tgacttccca gccctggtcg atggccttct ggtgggaggt
      181 ggcatagggg ccgatggcct cgacctccgc cttcgtgcgg gggaagtgga cgcagagatg
      241 gcccgcgaaa ttgttgatat ttttgttgtt gtaggtgccg tggggcgtgt tgtgggtgga
      301 ggccatgtag acagaaccgt cgctgaacat gacccagacg ggcttcgggt tccacgtggt
      361 cttgccgaac gccttgttca tcttcgccgt gtcctcggcg gtgatcggga tcgcgtccgc
      421 gtgagcaccg ttggagaaga tggtcagatg ccaggaaagg ccggtggaga agtcgtagac
      481 cgtcacgttg ggatacttcc gagccttggc gcggatctcg gtgtaccagt tggcatagcg
      541 gacgctggcc gcgctgatgg tggtcacgtt gggcgcggtg gtgggggtga cgatggtggt
      601 cgtgccgccg ttgttgacgg tgccgctggt ctggttcagc ttcttcgtgg tggcggaacc
      661 catgatgccg tccgccttca ggccgttggc ccgctggaag gcgatcaacg caaggctggt
      721 ctggttgccg aacttgccgt ccgccttgcc ggtcagatag cccagcgcga tcagccggtt
      781 ctgcatggcc ttgaccgctt cgccggtagc gcccttgtac agggtgacgt agggaaccgt
      841 gttggaaacg gctccgctgt ccgtggtgcc gtcgcccagc ttggcctgcg tggccgcgcc
      901 cacaatgccg tccggactca ggccgttggc ctgctggaag gccttcaccg cgcggcgggt
      961 ggaggaaccg aacttgccgt cggcggaaac gctcagatag cccagcgaga tcagcttatt
     1021 ctgcagggtc tgcacctcca cgccctggct gccgtagcgc agcgtggccg ccttggagga
     1081 ggcgatcacg tcggaattgg cgttgtcagc ccgcttggcc gcgttgctgt aaagcacggt
     1141 ctgagtgttg tagcccgcct tgccgtcggt ggtcaggccg ttcgccttct ggaaagcccg
     1201 cactgcggcg atatcgtccg tcagatagat gccgtcctga cgggaggtgt agtagcccag
     1261 ctcctgcaga cgaatctgca gccgcttcac atccatgccc tgactgcctg ccttgatggt
     1321 gacgcagttt tccttggtga tgggcgcata ggtaggttcg ggggtctcgt tggcggcgat
     1381 cacaggggtg gcggtgggca ccgcgtacac cgggtgcgcc gcaaacagca ccgcccgggt
     1441 ctgaatgccg catacgccgt ccgcgtccag cccattggcc ttctggaact tcttaagggc
     1501 ctttacgctg tcgtcatcaa aggtgccggt gactttgctg gtcaggtagc ccagctcggt
     1561 cagacgggtc tgcaccagct tcacatcatt gctcttgtcg cctggacgaa ccacgtcctt
     1621 gggcatttcg ggcacgggag tgggcgtggg ggtcacggcc acggaggcgt ccagcgccca
     1681 aatgctgtaa agaagattct gatcggtagc ggaaagcgcg ccatccacca ccagactgtt
     1741 cttgcgttca aattcctcaa tagcggacac cgtcagcgcg tcgcatacgc cggagggagt
     1801 cgaggtgtag tagcccagct ccttcagccg ggtctgggcc ttggtgacca cttcgccctg
     1861 gccgttgagc ttgatgaccg cacccgcgat gggcggaatg gtcttgacgt gctggcggaa
     1921 gctgttcaca tccttgggcg tgccttcgta gatcagcagc tgcagttccg cgtccgcctg
     1981 ccccgtctgc tccagcccgt tgcgcttctg aagcagcttg acggcctgct cggt
//
LOCUS       DAWAEA010000251         2032 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7512_length_2032_cov_3.083966, whole genome shotgun sequence.
ACCESSION   DAWAEA010000251 DAWAEA010000000
VERSION     DAWAEA010000251.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7512_length_2032_cov_3.083966"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    268..277
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          410 a          634 c          504 g          474 t
ORIGIN      
        1 ctcccggata gcgctgtcct ccatcgaaga aatccagaga cgaagaattg gcttgcggca
       61 tccagccatt tcatacacca gccggaagat cagctcacct tcacgcccgg catcacaggc
      121 attgacaatg gtatccacat cggggcggtc catcagcgcg cgaagatttt caaaagcgtc
      181 ctccttgccc ggtgccagca cataacggaa cggctccggc agaatgggaa gatcgtcata
      241 acgccatttc ttgaaatgct cgtcatannn nnnnnnnagc cacccgcatc catcggggat
      301 accaggtgcc caacgcacca ggatacgaga aggccgctgc cctcataaaa gccatcggcc
      361 ctggatgaag cacccagggc cgacgcgata gattttgcta cgctcggttt ttccgcaata
      421 atcagtttac tcattctttt cctccgtttc ctcgtcctcg gtcagatatt cttcttcatc
      481 ctccaggtcg aaatcatcca ggtcagaagg gactttctta ccctgtttcg gtttcagcac
      541 caggaaatag accgccgcgc cgcctccggc tgcaaggatc agcagcaccg ccaggatcgc
      601 cccggtattg cttttctttt cgggttccgg attctccggt tcggtgggct ccgcagcttt
      661 ctgttctgtg ccaacacatt cactaaggtt ttttacgcag accgggcaag cggtattgac
      721 cgctccggcc tggcacttct ccgtacaggt gcagacaata ggtgtttccg ccttttctcc
      781 atcctccgtg agtgccatga gatcggcttc atccacctgg ttcagaaaat gcacggtttc
      841 ctcgccctcg tcgtcacggt cgataatgag atagaacacg ttaccgttct tcgtttccac
      901 cgtaatgaac tgcttgccgc tccgagtgct gccgccgata tcgtcgatca gcgaaaggtt
      961 tccgtccggc gtcagggcgg caccgccgct gtcgcccata agctgctcaa acagctcgct
     1021 caatttatcg ggatcaatgg agaaggaaaa gctgctgtca ttttcgccgc ccgtatcgct
     1081 gagcgtcata tagtcggaat aaacatagcc gatcttctcc ggcagccgga ccatatacca
     1141 gtcgccgtcc gtgccgacga cctctaccac cgtcccgttc ggaagctgcg taaaagcgta
     1201 gttatccagc ccggcgcccg tgcgaacatt cagcttgccg ccgttggtgg tgacgattcc
     1261 tgtttttccg gatttctccg tgccaataga gccaaacagc gcggagatca gttcggagag
     1321 atcgtccccg gtgatatccg cgctgtcagt cggttccgtc tgtgcttcgg aggcagggac
     1381 tgcatccccg ctgcttgcaa aagcgaccgt agaaaaggcc gtcatgcaga gcataacggt
     1441 cagcagcacc gtaagtgtgc ggatcaggtg cttattcttc atcgtcggcg ccctccatca
     1501 tgtcgggatc agcggcgccc tccctttcca tcatcccttc cggcagggag attattccgc
     1561 tggcgtaggc gcgcaggaag gcggtcagct ccttgttgtc catgtggaca gcctttacca
     1621 tgcggacgat ctccagattt tcttcctccg caagctgggc ctccagcgca cgaaggcgtt
     1681 tctgctgctc cgtgatcttc tcccggacct tctcaatatc acgtcggatc ttctcagtag
     1741 ttgccatagt caaataaact cctttccaaa tcgttctctc catgaatagt tgcgtccaag
     1801 cccctgaacg gttttcaggt tggcccgtgc gttatcctcc aatgccagcg cacgccggaa
     1861 caaatccggg taatgctccc gcagatcgat gatctcctcc gcccgcatac tggggcagaa
     1921 aaaacaggag gactttcccg gctgcggcag gccagccgcc tcgattgccc ggatacacgc
     1981 ctcccggtcc cagccccatt ccatcagggg ataccacttg ctgtacttcg gg
//
LOCUS       DAWAEA010000252         2030 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7526_length_2030_cov_2.457215, whole genome shotgun sequence.
ACCESSION   DAWAEA010000252 DAWAEA010000000
VERSION     DAWAEA010000252.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7526_length_2030_cov_2.457215"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1264..1273
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          372 a          597 c          627 g          424 t
ORIGIN      
        1 gggaagctcc tcttgcattt ccgtaccgtc ctccgcagag tggagaaggc tcacataagt
       61 gccgtcgtac tccttataac ggagcgtttc cggggaggtg gtcagcacgg cgccgtcagg
      121 gtaggtgtaa gagccggaac gcagcggctt tttgcggaaa agagcaggcg caaattccgg
      181 ttcgccgctc tgattgaaca gagacacgtc cagttcagcg aacaggggga cgttgctttc
      241 ggcgtgaccg taacgagcca ttcttcgccc tcataggttt catcccgccg gtcgtattcc
      301 cagtgggaga cccacaggtg cagcgtgtag ctttctccgg cggtaacggc cgtgcccgcg
      361 tcgatttcca gcgtgtaaag gacgctgccg tcctcctgcg ggtggctgga cacgccgtag
      421 caggcggggg aaagcgccgg gccgccgtcc acggcgatct gatccacggc gatttcggca
      481 tagatcagct ttgcgccggt ctcggcggct ttttcgccgt aggtgggcgc gtccggaccg
      541 gggtgaatgg cgttgtagcg gtcggtgccc acgggcgtgt cgggatcgtc ctcttcctgg
      601 ggcagcagca cgatcttcgt gccgtccacc gggcggatgg tcgccacggc atacaggccg
      661 ctgtcgatgt aggtgacttc gtgcaatgta tagcgcacac catccagatc aatgctgctt
      721 tccggctggg cgctcttgcc tgcctgcagc ttttcctgcg ccgcagggcc ataggccgcg
      781 ccgaacacct cggccacccg gggagagaac accgcgtagg ctgcggcggt cagcagcgcc
      841 aatagcagcg cgcacaccag cgccagccgc agggcgcgcc gcaccggcgc gcggtgggga
      901 gcatggtcgg gggactgagc cagtgtctgt tccacgctct gccggaagct ctccggcgtg
      961 gagccgtaca gttttttcag gttttcagcg ttcatccgtc tacctccttt aacgcaaggc
     1021 tgctgcgaag ggcctgccgt ccccggtgaa gccgggattt cagcgtgcct tgaggaaggc
     1081 gcagaatgcg cgccgcctcc gctacggaat agccctccac gtaatacaaa accatcggca
     1141 gacgatattt ctcctcaagg gacgtgaaca gttcatacag atcgggcaga gcgtcagggg
     1201 ccgtttcccg ctcgggcaga tcgttcatgg gcgtttcccg tttgcggctg cgcaggatca
     1261 ggtnnnnnnn nnngatcgcg cagcttttcc cgcttccgca gcgccttttc gatggcgctc
     1321 tggacagcgt cctgccggtc gcaaagctgg ggcagaaggg tggtggacac gcggtagagc
     1381 gtatcctcca tggccacaac gcgccggaca aattccgctt gtgtcatatc gtacctcccg
     1441 gatggaatgg atgaaaagca cttttcaccc atacagatgt atgacaatgg caaaaggttg
     1501 caaaaaaaac ggctctttaa gagaatttcg cggaaatatt gactttccct gttcggcgtg
     1561 ttacgattcc tgacggaaac catatgatgg cttcttcggg gcggggtgaa attccccacc
     1621 ggcggtacag cccgcgaagg agaaggaacg ccgttccgtt ctccccgatt cggtgaaatt
     1681 ccgaagccga cggtaaggcg cagcgcgcca agtccggatg atagaagaac agccgcctgc
     1741 aaggtttttc gtatgcaatc caaatccaaa cgtctcacga cccgtcaact gactaccatc
     1801 gccattctgg ccgccatttc ggcggtgctg ttcatggtgg agatccccgt ggtgctgttc
     1861 tacaagctgg atttcagcaa tctgccggtg ctgctgggag cctttgccat ggggccggga
     1921 cccgccacgc tgattctgct catcaagagc ctgctggggc ttttgcacac gtcctctcag
     1981 ggtgtgggcg agctggcgga ctttctcatg ggctttgcca tgatcctgcc
//
LOCUS       DAWAEA010000253         2029 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7534_length_2029_cov_1.122594, whole genome shotgun sequence.
ACCESSION   DAWAEA010000253 DAWAEA010000000
VERSION     DAWAEA010000253.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7534_length_2029_cov_1.122594"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1335..1344
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          410 a          600 c          557 g          452 t
ORIGIN      
        1 gaacaaagca ccaggctgcg catgatcggc gtgctggcca ccggcttcaa cgtggtggac
       61 attgacgcgg cggcggaacg gtgcattccc gtttgcaacg ttccgtccta tgccacggag
      121 gccgttgcac agtatacctt cgccctgcta ctagaactct gtcaccgtgt cggggcccac
      181 gatcgagcgg tgcaggaggg ccgatggaca cagggcaaag aattctgctt ctgggattat
      241 ccccaaatcg agctcaatgg caaaacaatg gggctgatcg gttacggacg catcgggcag
      301 gcagtcggac ggattgccgc cgctttcggc atgaaggttc tgcacttttc gccctccggt
      361 cgggaaggcg gtgattctct ggaagaagtg ctgcgccaaa gcgacgttct ttccctgcac
      421 tgcccgttga cagcagaaaa ccatcatctt atcaatgctg aaaccctttc catggtgaaa
      481 ccgggcgttc ttctgcttaa caccgcacgc ggctccctga tcgacgaagc cgctctgcga
      541 aaggcgctgc tgaatgggca ggtagccggg cccgccgtag acgtagccga gcgggagccg
      601 atcccggcgg acagtcccct gctggggctg aacaactgtc tcatcacgcc ccatattgcc
      661 tgggcggcca gagaggcacg ccaacggctg atggatactg tggtggagaa cgtccgcgcc
      721 ttccagcagg gaaaccctgt caatgtagtc aatggagtga gtctttcccg ttaacgcccg
      781 tttcatcttc catctatccg atttttctct gttttccctt ccgtctcccg caaggcactg
      841 ttcctgcacc gttttcccct cattttccct tttccattcg tacacgcgcc tgatattcct
      901 tgggcataca gcccatgtat ttccgaaaca ccttggaaaa ataataggcg ttttcgtagc
      961 ccagccggtt ggccacctca tagatcagat ggtttcccgt ttccagcagc tcgcaggccc
     1021 gtttcatttt catctgattc acgcaatcca caaagcccac cgaaagctgg cgcataaaca
     1081 gggaagacaa ataatttggg gaaatgtgca ccgctgcgct gacatcgttc agggtcaggc
     1141 gctcctccac gtgctcctga atgtagcttt tcgccataga ccagcttgat gcggctgcgg
     1201 acattagccg cgccgatgcc gcttttgtcc gcagattcca cctgtccgtt cagcacggcc
     1261 agccgctgtt cgtccatgcc cacgccgttg tcctctacca ccagatgcag cacaccgtcc
     1321 tccgccgtgg ccgannnnnn nnnntcacga tcttgcagaa atgctctgcc ggttccaccc
     1381 catgaaagat cgcgttctcc accaacggct gcagcacgaa tttaatgatc cgatagtcct
     1441 gtaagtcctc gggaacaaga atatcgcagt cgaaggagtc catatagcgt acggactgga
     1501 tggtgatata gtgccggagc aggttcagct cctctcgaag ggggatccgt tcgccgggcg
     1561 aacggaccag attgcgcagg agcgccgtca gggatccggt cagggtacag atgccgatat
     1621 tcttttgcaa tttcgccatc cagtggatgg aatccagcgt gttataaatg aagtggggat
     1681 aaatctggct ttgcagggcg gcgatctcgc tgtccttttt ggcgttggct tcggccaccg
     1741 cctgttccat cagcccctga atgctgaccg tcatctgatt gacggtcgcg cccatctggg
     1801 caaagccgac gagcagctgt tccagctccg ggtcgggaga caagtcgttt tccgctatgc
     1861 gggccagccg gttccgcagt cggtcggcgg ggatggagat attccgggtg cagattctga
     1921 tgcagatcag ggccagcaac agcgttccca gcaggatcag acacagcatg gccgcaacct
     1981 gcatcctctc ctgccgggga gacgtgacct tcatgatgca ggatacggt
//
LOCUS       DAWAEA010000254         2021 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7564_length_2021_cov_2.388098, whole genome shotgun sequence.
ACCESSION   DAWAEA010000254 DAWAEA010000000
VERSION     DAWAEA010000254.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7564_length_2021_cov_2.388098"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          325 a          668 c          631 g          397 t
ORIGIN      
        1 cgtttgttta ttccttttgg gctgccaacg cggcaagtgc ttcgtgcacc agctgggaca
       61 gtcgttccgg gtcggcccgg ccccgggtca gggccatggc cttgcccata agggcttttt
      121 ctaccgtgag tttgccccgc aggtagccct ccaccatgga gggattgtcc cggagggcct
      181 gctccgctgc ctgacggaga acattctcgt ccgtcacaag gaacaggccg tgttcctgcg
      241 cgtaagtctc tggctccacg tcctcgtcaa acagcgcggc gaggatcttt ttgccggtag
      301 agctgttcac ccggccttcg tccagcagat tgctgagcgc cgccagacgc cggggcggaa
      361 tgggcaggct ggtctcgttc ctctcggttc cgggcgtttc ccgcagggtc agccgggcga
      421 agacctcgcc caacagcaga ttggccagcg ccgctggatt ccgagcgtca gccgccgccc
      481 gcataaaata atccgccagc cacttttcgt ccgtcaaaag ctccgccgcc ttgcggggca
      541 ggccgtactt ctgctggaaa agggcgcgtt tttcgtccgg cagcataggc agcgaagccc
      601 ggagttgttc cagctcctcc gccgaaaaga ccaccgacgg caggtcgggt tccggaaaat
      661 agcggtaatc gcccgtcgtc tcttttcggc gcatgacgat ggtcttcccg gtgcgctggt
      721 caaagcgcag agtctcctgc agcaccgtgc cgcccgcgtc cagaatttcc gcctgccggg
      781 cgtattcggc ccgaatggcc cgttccacgc tctggaagga gttcagattc ttcatttccg
      841 tgcgggtgcc catgggctgg cccggacggc gaaggctcag gttcacatcg cagcggaggc
      901 tgccctcgtt catgcggcag tcgctgaccc cggcggcggt caggatggcc cgcagctttt
      961 tcagataggc gctggcttcc tccgccgtgc gaaagtccgg ctcggtcacg atctcgatca
     1021 ggggcacgcc gcagcggttc aggtcgatag ccgttacgcc gggccggtcg tgcagcagct
     1081 tgcccgcgtc ttcctccacg tggatgcggg taatgccgat gcgtttcgtt cccccatctg
     1141 cgagaatttc cacaaatccc gcttcgcaca gggggtggaa aaactgggtg atctgatagg
     1201 ccttgggcag gtcgggataa aagaaattct tgcggtcgaa gctgctgacc gggttcaccc
     1261 ggcagttcag ggcaaagccc gccagcgcgg ccagctccat ggcccggcgg ttcagcacgg
     1321 gcagcgtgcc cggcagcccc atgcacaccg ggcagaccag cgtgttgggc ggtgctccgt
     1381 agcggttttc gcacccgcag aacgccttgg tgcgggtctt cagctccaca tgggtctcca
     1441 gacccaccac cagttcatag cggttttccg tcatgcccgc gctccttcct gcccggccgt
     1501 caggcgttca tagggatatg agcctttggg gagcggacat tccgtttcat accgggccgc
     1561 cagccggaac agcaggggca gcgattcccg gccgcccatc agcgtcatgc ccatgggaag
     1621 cccctccccc gaaagtccgc agggcaggct caaagccgga agccccgcca gattggcggg
     1681 cacgctgtac aggtcagaca gatacatctg ggcggggtcg tcctgctttt cgccgatggg
     1741 ccacgccgtg gtggccgtgg tggggaacag cagcgcgtcc gcggatccca gcacccgttc
     1801 cagctccttc tgcaaaaggg cacgggcctg cacggcacgg tcatagcttt cgacccgttg
     1861 ttcttcggtc aggctgtacg cgcccagcaa aatgcgccgc ttgacctcca tgccaaagcc
     1921 ctcccggcgg ctttcccggt acagctggtg gatttcctcc gccggggcac gatggccgta
     1981 gcgaacgccg tcgtaccggg ccagattgct gcccgcttct g
//
LOCUS       DAWAEA010000255         2013 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7595_length_2013_cov_1.904494, whole genome shotgun sequence.
ACCESSION   DAWAEA010000255 DAWAEA010000000
VERSION     DAWAEA010000255.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7595_length_2013_cov_1.904494"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          343 a          553 c          677 g          440 t
ORIGIN      
        1 cccaccggca gctggagcag atcgagtact gtctggaaca cgacagcatc ccctgcgtgg
       61 tgtcgggccg ggaaaacttt tgggaggacg gcaaggtacg gggcatgttg ggctttttcc
      121 gctccctgca ggagccggaa aacattgcgg ccttgagcga cgcgctgtcc cttttgtgga
      181 aagttcccgc gccgctgatt cagcgggcgg gggagacttt gcggacagcg aaagagccgg
      241 ccgcccttcg ggaaagactg gcttcctttg aggtgctggc cccgtggctg gacgcggcgg
      301 acgagcttcg gccccagctg acaaaggcaa agccccggaa attgctggaa cggctacggg
      361 agctgtgcgg gctggaaaat tccaagccgg tgtcccggct gctgaacgcc tccgtgttcc
      421 agaatcgaat ggaggaattt ttggaacgga cgctgctggg cgcggagggc gacctgcagc
      481 gccggggcga gggagccgtt caaagcggcg cggtgcgtct gatgacgctg cacgcctgca
      541 aggggctgga attcccggtg gtctttctgg cgggcctgaa cagcggcgtt ctgccgctgg
      601 agcgggagaa aagtcccatg aatgtggaag aagagcggcg cctgttcttc gtgggcatga
      661 cccgcgcccg ggaggaactg atcgtcagct atggcggtca gccgtcggcc ttcgtggatg
      721 aactgccgga gagcattcat ccgcagccgg ttcgcacccg gaagcctatg gtgaagatgg
      781 agcagcttag cctgttctga caatgatttt gaatgcaaaa gagggaaaag catggtttcg
      841 ttgttgctgg cggttattta tctcgccttt atcagtttgg gcctgccgga cgcgctgctg
      901 ggctccgcgt ggccggtgat gcagggggcg ctgaacgccc ccctttcctt tgccggagcg
      961 gtctccatgg ttatttccgg cggaacgatt ctctccagcc tgctgtccga ccgcatgacc
     1021 cgaaagctgg gcgcgggact ggtgacggcc ctgagcgttt tgatgacggc tgcggcgctg
     1081 ctcggctttt ccctgtgcag cgcgccgtgg atgctgatcc tttgggctgt tccctacggt
     1141 ctgggggcgg gcgcggtgga cgcggcgctg aacaattacg tggcgctgca ctattcctcc
     1201 cggcacatga gctggctgca ctgcatgtgg ggcgtcggcg ccagcatcag cccgtttatt
     1261 atgagcctgg ccctgcagcg aggctggggc tggcagatgg gctaccgctc ggtctcctac
     1321 atttagctgg ccctgacggc gctgctgttt ctcagtctgc ccctttggaa aaagcggacg
     1381 aatgagacgg cggaaagcaa cgaatccgtt gaaccggcca aacgtcagag catcggactt
     1441 tctgggcatt acccttggcc gttttctgaa tggatttatt gcggatcgtt tcggcgacaa
     1501 gaccatgatc cgcgccgggc tggggctggc ggcggtcagc attctgatca tcctgctgcc
     1561 gtggcagctc tgtccgctga ttggtctggt gacgctgggc attggcagcg cgccgatcta
     1621 tccctgcatc attcattcca cgccagagtc ctttggccgt gaaaattcac aggcgatcat
     1681 cggggtgcag atggccagcg cgtacatcgg ttcgacgttt atgccgccgc tgttcggcct
     1741 gatcgcccaa tacggcaata tcgggttttg cccggtatat ctgatgatct ttgcggtttt
     1801 gatgatcgtt ctgtacgggc tgtttacgaa ggggcaggga aagaaggcgt aaggaaactc
     1861 ggttcggcgt ccgaaggggt gcgggggcgg aaagccgcct gcctgtgaca gcgtgcccag
     1921 tatggctgta agaaacccct ttccggggct tgacgcgccg acttcggcgc gaattgggac
     1981 ttgccgtccc atggggcgtt cacacttttc ttt
//
LOCUS       DAWAEA010000256         2010 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7612_length_2010_cov_1.907417, whole genome shotgun sequence.
ACCESSION   DAWAEA010000256 DAWAEA010000000
VERSION     DAWAEA010000256.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7612_length_2010_cov_1.907417"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          405 a          670 c          548 g          387 t
ORIGIN      
        1 gtccggggtg caggggcact gcccctgcct accacatttt ggccattttg gagagcttgt
       61 ccacctgctt ctgaacctgc ttcttcattt tctggttgcc gggcatcagc atcgcacccg
      121 cgccgaccgc gaatccgacg gcccccgcga ccgccgcttt ccaagcgctc attcccatgc
      181 ctcccttctg gaacgttccc gaaattccgc ccatgtcatc agctcggata ccatcgtctg
      241 ctttccgtcg cctgccaccc ggtaggaaac cgtgtaggca cagcgcccca gaagcgcgta
      301 aaccggcccc ggcgtgacct ccagcgccac cagccgcagt gtcagcccgc tgaaaagcgc
      361 gtcgctgacc agccccgccc gctcgccggt ggtcagatag gcttgcaggg cggaatccgt
      421 caggccgtcg ggcagcggct ccggcttggc cccggccaaa acgcaggtgc gccccaccag
      481 acgaacgcta ctggaaggca gccaccgcgc caggccgatg cctttacgga tcaccagccc
      541 ccgcagccgc cgggcggatg cgtccgcgat cgcccgttcc acacatccga tctgccggtc
      601 ctgccagatg acgggcattc ccgcgattcg attcagactg gtgagcatcg cttcgttctc
      661 ctttccgcgc cgtccgcatg aacgtatggc tatcttgccc cggccgacgc ttttttcgac
      721 aaacaatgct tgccagcgcc gccctctttc gtccgtaaag gaagaaaagc gaaattgcaa
      781 actcaaaggc cgggaaattt ctggaaggag acttttctca aaagccgacg ctccttcttt
      841 tcccaaacac aggatggaaa cggttggaaa aaacgcgctt ttctgcccct ctgattggct
      901 tggatccctt tattttccag tatttttgtg catgaagccg ccctctctgt tcgtcggata
      961 ggcgaatggc acggaaaacc aaccgcgcca taaaagaaaa ggaggtattt catcatgcaa
     1021 aagacccgtc tatacaaaag atgcctcccg gtgatcgctc tggttctggc tctggccctt
     1081 ccgctggttt cgctggcgga aagctataag gtcgtgaccg gcggcggtct gaatctccgc
     1141 aaggagccca gcctgaactc ttccgtgctg ggccagtacc ccacggtaac ctggatgacc
     1201 gtgctggaaa ccaccggcga ctggagcaag gtgcaggtca atggtaagac cggctacgtt
     1261 ctgtccaagt acctgaccga ctccaacgtc aacaataccc tgaccgtgcg caccaacacc
     1321 ggcatcggcc tgaacgtgcg caccgcgcct tccctgaccg gcgatatcgt gaccagcttc
     1381 aagaacggct ccaccgtgac cgtgctgagc aagggcaacg gctggtataa ggtgcagcag
     1441 ggcgacgtgg ccggctacgt tgccagccgc ttcctgtccg gcaacgcggg cagcaccacc
     1501 aagcccagcg gcaagcaggg cgtggtgaag aaccccggcg cgaatcaggt cctgctgctg
     1561 cgggaaaccg ccagcaccga cgcccgcgtt ctgggctatt accgcaacgg caccgccgtc
     1621 accattctgg gcgaaagcgg cagcttctac aaggtgagcg taggcggcaa gtccggctac
     1681 atgatgaaga agttcatctc cgtggtgggt tcttcctccg gcgctgcctc caccctgccg
     1741 aaggccccct tcaccgccaa gctggtgaac cccaacggca acggcatcgt gaatttccgc
     1801 aaggcccccg gcctgagcgc ctccatcatc aaggcgtacc ccattggcac cgagattcag
     1861 gtgaccgaag tgggcgatgt gtggtgcaag gccaccatcg acggcaccga aggctatgtg
     1921 agccgctact tctttcaggt cgtgaaataa gacggctttc ccttcccaac agcaaagacc
     1981 agcgtgttgc gaatacacgc tggtcttttt
//
LOCUS       DAWAEA010000257         2007 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7621_length_2007_cov_1.877561, whole genome shotgun sequence.
ACCESSION   DAWAEA010000257 DAWAEA010000000
VERSION     DAWAEA010000257.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7621_length_2007_cov_1.877561"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1286..1303
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1666..1784
                     /estimated_length=119
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          385 a          514 c          631 g          340 t
ORIGIN      
        1 acagatggtg gagatcgaga agctgacctc cgagcacgat atggaggccc tgcgcggtat
       61 gatcgaagcc catgtgcggg agaccggctc cgaaaagggc cgccgcctgc tggaaaactt
      121 tgcggaattc gccggaaaat tcaagaagat cataccccgg gatttccgca aaatgctggg
      181 tgccatcgcc cgctacgaag ggcagggcat gaccagggag gaagcggaac tggaagcgtt
      241 ccgggaaacg acggagggca ggtgagcgca gatgggaaaa aaagatggat ttttgctcta
      301 tgagcgccgc aatactccgg cgcaggagcc tgccgggcgc atcggcagct ttgaggagtt
      361 tcatacgccg ctgccgctgc aggagcggcg caggcaggcg gcccgctgca tgaactgcgg
      421 ggtgcccttc tgccagagcg gcgtggagct gggcggcatg ataacgggct gcccgctgca
      481 caacctgatc ccggaatgga acgatatgct gtatctgggc aatacccgcc atgcgctgga
      541 tcgcctgacc aagaccaact gttttccgga gttcacgggg cgggtctgcc cggcgctctg
      601 cgaagcggcc tgcacctgcg gcctgaacgg cgacagtgtg accgtccggg aaaatgagct
      661 ttctattatc gaagaagcct tcgaaaaggg ctggatgcag ccccagccgc ctcggacccg
      721 ttcgggaaag cggatcgcca tcgttggaag cggccactcc gggctggcgg cggcttatgt
      781 gctcaaccgc cggggtcatt ccgtgacggt attcgagaag gcagaccgcc ccggcgggct
      841 gctgatgtat ggcattccca atatgaagct ggacaagaag atcgtggagc gccgcattcg
      901 cctgatggag gccgagggcg ttgtcttccg taccggcatg aaggtggggg aagacgtttc
      961 cgccgatgaa ctccgccgga cgtatgacgc ggtgctgctt tgctgcggcg caggtcagcc
     1021 ccgggatctg aagctgcccg gccgggatgc ggagggcgtg tatttcgccg tggactttct
     1081 gaccgccgcc acccggcatt tgctggacgt tacgccttcc atcgatgcaa agggcaaacg
     1141 ggtggtcatc gtgggcggcg gcgatacggg caacgactgc gtaggcaact gtctccgcat
     1201 gggctgcgcc agcgtcaccc agctggaaat gatgcccaag cccccggaaa atcggctgga
     1261 aagcaaccct tggcccgaat ggcccnnnnn nnnnnnnnnn nnnaccagtg acttcttcgt
     1321 gggctggtgg tggctgcttc tgctgatcgc cgccgggttt gtggggtgca tgcccatggt
     1381 gccggaagcg ttcggcgtgg gcgtgacgga acgcggacgg gtggtcacag aggactatca
     1441 gacggaagcg ccgggcgtgt tctgcgcggg cgatatgcga cgcggacagt cgctggtggt
     1501 ctgggccatc cgggagggac gggaagccgc ctgccggatc gaccgatatc tgatggggta
     1561 cagtgaattg gaaggctgaa aaagaaaaaa gaaagagcgg cgcgcctaca tgaacgcgcc
     1621 gctctttctt cgttgacaag ctccgtcaac gtttctgccc gaaagnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncttgtt tttgtgtttt
     1801 gaaaaaggaa ccgaaattat tcgccctcag cggactgaat ggaagccatc acgaaggaga
     1861 ggatggcggc aaagccttcg tcggacttcg gagagcctgc aacctccaga acatatccgg
     1921 cttcggtcgt aaaggcgatg caggcggtgt cgctttcttc cagaacgtag gtcacagcag
     1981 gcagaccatt cacggtcacg tcctcca
//
LOCUS       DAWAEA010000258         1984 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7716_length_1984_cov_1.556765, whole genome shotgun sequence.
ACCESSION   DAWAEA010000258 DAWAEA010000000
VERSION     DAWAEA010000258.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7716_length_1984_cov_1.556765"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    280..307
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    743..758
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          393 a          582 c          629 g          336 t
ORIGIN      
        1 gaagcggaac tgtactgcat ccacacgctg ggctttcgcg gtgaggcgct ggcctccgtg
       61 gcggcagtgg cgaaagtgac ttgcaccacc aagaccgccg acgcggagtt cggcgtgcgc
      121 tgtcaggtgg agggcggcga gtttaccgac atccgtcagg ccgcctcgcc ggtgggcacc
      181 accttcgtgg tggaggattt gttttatacc ccccccgtcc gccgaaagtt cctgaaaaag
      241 cccgccattg aggccgggct ggtgtcggac tatatgctcn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnngca aggacgcttt ccgccagatg atccccgtca gcgggcacca gagcggcgtg
      361 gccgtcagcg gatttatcgg cgtgggggag ctttcccggg gcaaccgtca gcagcagagc
      421 tttttcatca acggacgttt tttccgcagc ggcgtattga gccgggcgct ggaaaacggc
      481 tgcgagggca gggtcatgat cggcaagttc cccatgtgcg ccctgtttct ggaaatgccc
      541 tatcagaacg tggacgtgaa cgttcatccc aataagctgg aggttcgctt tcaggacgaa
      601 agcgccgtgg cggaggccgt ccggcagatc gtctatgacg cgctgcatca ggaacagctg
      661 ggccagcggc ttcgcgcccc ggcttcggaa aagcctgcgc ccgttacgga aagcggtttt
      721 accgtgacgg agctgcccgg cgnnnnnnnn nnnnnnnnca agacgacgcg gcattagaac
      781 agaaagacga agctgccgca gagaatacag cgcatcttcg cccagtgact gtgcccgttc
      841 agacgggggg cggcggcgcg ttccatgcgc cctacggcgc agcaccccgg acggtctttc
      901 agcccgcggc cccgtccgtg tgggatcagc cccggaacgc ggctcccaag cccgccatgg
      961 gatgggggct ggctgaaaag cagtccgcag cgccggagat ccgggtaacg tccgcgcagc
     1021 cggtgtttgc caagaatgag gaaaccggcg aacccgctcc ggtgctccct gaaacgcagc
     1081 ttcaaccgcc cgaggctgtt cccgatgagg cgttacagcc cccggagcag acccgccttg
     1141 cggaggtgga gaccccggaa gaggccgccg ccgttcccag gctggtgggc atcgtgttcg
     1201 atacctattg gatcttcgag accggcgacc gtctgttgtg gctggatcag cacgccgccc
     1261 acgagcgcat tctctacgac cgtttcatga agcgctacga cggcgagcag atctctcagc
     1321 ggcttctgtc gccccagctg gtgcagctct ccggccggga tatggcgctg tttgaggaaa
     1381 ttcagcccta tctggaaaag gcgggctttc aggcggaagc atttgacgag cgcagcgtgg
     1441 ccgttcacgc cattccgacc ctgttcggcg aaaacggcga cccgaaggaa ctgtttctgg
     1501 aagcgctgga tcagtggcag cagggccggg gggaaaagac cagcgaccga atgcgcagtc
     1561 aggtggctca aatggcctgt aaacacgcca tcaagggcgg cgacaggctg aatcagcagg
     1621 aaatacaggg ctttctgaag gaaatgctgc aaagccagtc catgcccacc tgcccccacg
     1681 ggcggcccat cgtggtggag accacccgct atgcgctgga aaagcgcttc aagcgcattc
     1741 aataatggaa acacagaaag gaatgccgtc gatgaagccc aaggttctgg ccctgacggg
     1801 atgcaccgct tcgggaaaaa gcggccttgc cctgaagctg gcccgggaat acccggtgga
     1861 gatcgtctgc atggacagca tgcagattta ccggcgcatg gatattggca ctgccaagcc
     1921 cacgctggcg gaacagaagc tggttccgca ccacatgctg gacattttgg agccggagga
     1981 aagc
//
LOCUS       DAWAEA010000259         1981 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7728_length_1981_cov_1.705607, whole genome shotgun sequence.
ACCESSION   DAWAEA010000259 DAWAEA010000000
VERSION     DAWAEA010000259.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7728_length_1981_cov_1.705607"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1444..1453
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          365 a          573 c          644 g          389 t
ORIGIN      
        1 ggtcagcgcg tcccgcttgg aagtgggggg cagcgtatag ctcaggcagg tgacgatgcg
       61 gcagaagtcc gggtcgctca ttccggcccg ggccagcagc gccagatcat aggccgtcgt
      121 atagtgtcct tcatcgtgat agccgtgcgg attagcaaaa tgggagtctc ccatgccgat
      181 ctgcgcggct ttttcgttca tccgttccac gaaagcctcc acactgccgg cttccagcac
      241 ggcgacggca ttggccgcgt cgttgcccga acggatcatc agtccgtaaa gcaggtcgag
      301 cttgcgcatc tgttctccgg gcgtaacggg caccagcgaa ctgtctgccg gaacgtccgc
      361 cgcctcctgc gggatggtga cgatgtcctc cagaccgccg ctttccaggg ccaccatcag
      421 cgtcaggatt ttggctcccg gctggcagac tcgaacaaca cttcgccgct ggggccgtcc
      481 atcagtatgc agctcttcga aaaaagataa ttcccgttca gcgtatcggg ccgggtgaag
      541 tcgtaatact ccggggcctg ccagccatag gaggacaggt cgacgttcaa caggcggacg
      601 gtgtccgggt cagccaaacc gtccggctcc cgctgaaaaa tgccccagcc ttttgcttct
      661 gcctgaaact tcgtcaccgc cgccttggtg gacgaaccga acgctccatc agccatttca
      721 tggagaaaac gaaggtcgat cagccggttt tgcagcgccc ggacccgctc accccggctg
      781 cctgtgcaca gggtggaaag cagcgcgtcg ttgctttcat caaacaaaag ctctatcgtt
      841 ttttgacctg caacgccgtc ctgcgtcagg ctgtgtccgc caagctccgt cagcagccgt
      901 tgggcttccg tgacggcggc ggaggtttgc ttgccgtaga tgccgtccgc cttgccgctc
      961 agcaggccca gttcgatcag cctgcgctgc agctgcgtga ccgccgcgcc ggaatcgccg
     1021 ggctggcagg tctcggcagc caacgccccg gcaggtagaa gcagacacat gcacaaaatc
     1081 agcagcaggg cgcgtttcat cttcggtagc ggctcctttc ggtgggaaaa atcggttctg
     1141 aaaatataga cgaatgaaac ccgcggaatg ttgcataggt tttggcggag ggatgcagta
     1201 tgaaacgggg ttatccgccg gtatggctgg ggattcttat attgacgctg gctgttttct
     1261 ggcgaatggc gggcgcgccg gtgacggtgg aggagtttgg agccgtggaa acgcccttct
     1321 ggcagatgag agtgctggct cctgcccgga tggcgcggat cctgcgcttg tggttttccg
     1381 ccccggcgga accggccagt gatctgcttc tccgggagga tgcagaggaa gaaggtctgt
     1441 cgcnnnnnnn nnngcacagg cggtcgccgc ccgtacccga gcggtgcggc agcagctgga
     1501 cgggggctgc agccgtcatc cgggtgcgga tatctgcggg gatagcgcct gttgtcaggg
     1561 atacgtgggg gaagcggcct gccgggaaaa atggggcggg gagtttgaac tctaccgcaa
     1621 acgggtgatg caggccgtgc ttgaaacgcg ggacgagctt ctccgttacg acggtgagcc
     1681 cattaccgtg atgtatcacg ccatgagcgg cgggcacacg gaggacgtac aggcggtgtc
     1741 ttcccaaagc ctgccttatc tggtcagcgt ggacagcgcc ggggaggaaa gcgcccgggg
     1801 attttatacg gacgcgactt tctcctacga agaagcggct cgtctgctga atgcctactt
     1861 cagcggcctg aatctgacgg cccagcgtct gcggcagacc ttctccgtgg cggcctatac
     1921 cgacagtgga cgagtcaagg cggttgtggc cggggaacat acgctggacg cggcggaggt
     1981 t
//
LOCUS       DAWAEA010000260         1978 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7741_length_1978_cov_2.089964, whole genome shotgun sequence.
ACCESSION   DAWAEA010000260 DAWAEA010000000
VERSION     DAWAEA010000260.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7741_length_1978_cov_2.089964"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          388 a          664 c          570 g          356 t
ORIGIN      
        1 gtgctcgtcc gtgccggtca ggaaataggg gtcatagccc tccagcttct tgaagcgggc
       61 ggcggtgtcg gcggcgacgc tgcagtaggc gtggccgatg tgcaggttgt cgctggggta
      121 gtaaataggc gtggtgatgt agtacttctt ctggttttcc atgaggctct ctctccttct
      181 ggccgttctt ccttcggcat tttttccaaa tagaccggcg atgatttttg aaagtttccg
      241 gcaatgaaaa agcctctccc ggtcattgcg aggaatattc attattatag cggtttttgc
      301 cgggctgtca atgggaaaac gcgagttttc agggctttca gccgccggaa agcagccttc
      361 ccgccagcca gaccccggcc agcatccccg taagggagca gagggccgca taacccgccc
      421 gtttgtcctt cacgccgctg aaatacacgc acagggtata gaagatcgtc tcgctggagc
      481 ccatgacgat gcaggccgtc atgcccacgg cgctgtccgg gccgaagcgc tccagcaggg
      541 tctccagcat gcccagagag gccgaaccgc tgaggggccg caccagcacc agcggagcca
      601 cctgagcggg caggcccagc agcttcagga ccggcgcgca aatatggcat agcagcccca
      661 gcaggcccga ttcccgcatc agggcaatgg cgcagagcat ccccgccaga ttgggcagca
      721 cccgcaccgc cacctccatg ccctgccggg ctccccggat gaagccctcc agcgcgtcca
      781 gccccttcca cccggcggcc agcagcagaa gcagaatcag cagaggcagc agcaggtcgg
      841 aaatcgtttt catgggcgtt cctcccggcg gcggctcagg cgataacaca aaagccccag
      901 tcccgcaccc accagcgtgg aaaaggccgt gcaggccagc gtgggcagca ggatggcgtt
      961 ggggtcggcg gaacctgccg ccacccgaag ggtcagcacc gtggtgggaa acagctgcag
     1021 ccccgtggcg ttcaaaatca gcagcatata cagggcgtga cgtacctgcg gattttgctt
     1081 ctgctcctcc tccatccgcc ggacggcttc catgccgctg ggcgtggcgg cgttgcccag
     1141 ccccagcaga ttggccgtca ggttcagcgt caccgcctgc cgggcttcct cgccccgcag
     1201 ggtgggcatg acccgccgca gaaaaggccg cagcagccgg gccagctttt cgggcaaacg
     1261 cagctccttc atgatctcca tcaggccgca gaaaaacaga tagcccgcca tcagccgcag
     1321 gctcaggctc acggcggacg aagccccgtc cagcgcggcg ggcagcagcg cccccgcccg
     1381 gcccgtcagg cagccgtaca aaagcgacgc ggccatcatc cccagccaga tccacgacaa
     1441 cagcaaaatg attccccctt ccgcggcttg tacagcctat gcggaagggg gcgggatgat
     1501 gtgcggccgg agggcttcag atccaccact ccggcatgag ccggtccagc gtgaccacgc
     1561 ggcccgtttt ccagtccagc agaatttcga ttttctgata gccgtcgggc atgagctgcg
     1621 ccatcatttc ccggcaagcg ccgcagggcg cgcccgtcct gccgtcgggc atcagcgcca
     1681 gcacccgggc gatcttctgc tcgccgcagg tcagcatgtg gaaaactgcg ttccgttccg
     1741 cgcagatacc cagcgtacag gccgtatcaa cgcatacgcc ggtataaatt ttccctgact
     1801 ccgatagaat agccgccgcg accccgcccg cctccacata ttgggagatc acacggccgt
     1861 tctgcacggc cttggcggca tcatacatct ctttccaaat cgcatccatc attttttctc
     1921 ctttgctgcg ataaaatttt accctcattt caaggaatcc ccttcgccta tccaaacc
//
LOCUS       DAWAEA010000261         1977 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7747_length_1977_cov_1.688345, whole genome shotgun sequence.
ACCESSION   DAWAEA010000261 DAWAEA010000000
VERSION     DAWAEA010000261.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7747_length_1977_cov_1.688345"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1585..1691
                     /estimated_length=107
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          368 a          564 c          569 g          369 t
ORIGIN      
        1 cccatcgagc gcatggtggc cgacgtgcct caggtctatg cgggtgcggt cattgagaag
       61 atcggccgcc gccgcggcac actggaaagt atgcacggcg tgaaccgggt gcgtcttgag
      121 tttctggttc cttcccgcgg cctgttcggc taccgcagcg agttcatgac cgatacccgc
      181 ggcgagggca tcatgagctc cgtatttgaa aagtacgagc tcgtcaaggg cgatattccg
      241 caccgcgctt tcggcgcgct ggtctgcttt gaggacggcg agagcaccag ctacggtctg
      301 ttcaatacgc aggagcgcgg cacgctgttt atcgggcccc agaccccggt gtacaagggc
      361 atgatctgcg gtcagaacgc ccgcccggat gatctggtgg tcaacgtgtg caagcagaag
      421 cacgtcacca acatgcgcaa cgcgtccgcc agtgaagaaa gcctgcgtct gatcagcgtt
      481 catccgctga cgctggaaga gtgtctggag tttatcgacg acgacgagct gctggagatc
      541 acccccaaga gcctgcggat gcgcaagcgg gagctgaacc acgagcagcg ggcccgtgcc
      601 gcctaccgcg ccaaacagta atgcccttac ccgatcgtga acgcaaagga gggaaccgca
      661 tgttccgtca agctgtccgc cgtgccctcg ccctgctgct ggccctgtgc cttttgcccg
      721 cagcggcgct gggcgaggcg gcctctctcg ccgaagaatg gcccgtggat tccagcgccg
      781 ttacccgcgc cgattttgac ctgagcgtcc agctccatgc ggacgctttc ccggccgacg
      841 gcctgacgga ttatcggggc tgggaaacct ttctcagcaa gcttcggctg tccggcgtga
      901 tcgatctgca gcgcttcccc cggcccaaca accgtatgta tatggaagcg cagctccttt
      961 tgaacgaccg ggagctgaca cagttttcct ttgaggatta ctggggcttc cgctacgtgc
     1021 gcagccccgc gctgtgcaac cgaagcattc atttccagat gaacaactat ctggaattta
     1081 tgctcaagcc ttatgaatac tacgagcttc ccacccagta tattgccctg ttgacctatc
     1141 ccgaagtgac ggcttatctg gcggaatgct atgaggacgt gctgactcct tacctgcatg
     1201 gcgagggaaa tcggacggtc tcctacgatg atctgtatca gatgtgtcag gagctggaca
     1261 cggtcatgac ggacgcgaag gcggagcagt cctacttctt cctgctggcg ttgctgatcg
     1321 atctgggcat ggccgatcag gtctacgacg gattaagcta tctggaagat tatctggatt
     1381 ggctggatcc cgaacaggaa ggcatgacca tcacggcgca gggcgatacc gaccgctacg
     1441 aggtgggcgg gcatgtcctg ttcacccgca cccggggaga aaacggcctg tccttccgac
     1501 tgtctctgcc cacggaaaac gacgaggagc tggcgctgga atacgaccag acccccggcg
     1561 cgaacggcgg cacggacgtg aatgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nctgacagtt tccgttgagg aggaaagcct gatcgacctg acgctgaacg
     1741 ccgacggtct tccgggcgag acggacacgc aggcggtcgg ccatctgaac tttgccgttt
     1801 ccggcagtgc ggtggaggag actgcctatg ctttccagtt ccggctgaca cgctccgccg
     1861 cccagccgcc cttccatgtg accttcggcg tggactggct gcatcccgaa acggagaagc
     1921 ccgccttcac gcttctgtac aacgccgatg tggaaatgaa ggacgaaagc gcgctgg
//
LOCUS       DAWAEA010000262         1967 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7786_length_1967_cov_2.994770, whole genome shotgun sequence.
ACCESSION   DAWAEA010000262 DAWAEA010000000
VERSION     DAWAEA010000262.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7786_length_1967_cov_2.994770"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    741..750
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          310 a          582 c          629 g          436 t
ORIGIN      
        1 gcggtgggac ggtaaatgtc gcacgcggcc agcagcggct ttttgccctg cttctgcagg
       61 tagttggcca gctttccggc catggtggtc ttaccggcgc cctgcaggcc gcacagcata
      121 taaatagtag gcacggaaga cgaccaggtc agccgggcgt gctcggagcc catcagggcc
      181 gtcatttcct cgctgacgat cttcatcacc tgctgggccg gggacaggct gtccagcacc
      241 tgctggccca cgcagcgctc ggtgactttt ttgcagaaat ccttgaccac caggtagttg
      301 acgtcggcct ccagaagggc catgcgcacg tcgcgcattc cctccttgac ggcctgctcg
      361 gtcagcttgc cccggccggt cagcttggag agcgcgctgg ataatttgga actgagaccc
      421 tcaaaggcca tgggcgtcct cctcctcttg ctgaatccgt tggatctgtc gggccgcctc
      481 gtcggtcagg gcgcgagcct cggtgggaat gtcctgcccg gcgatagcgc cgagcagctc
      541 ctgcacccgg gccagccgct ccagcatccg ttccatgcgt cccgccgcgc cgagggcggc
      601 ttcgcaggcc cggagctttt ccgaagagcg gacgatcaga tcgtgtacgt tctggcggct
      661 tacattcatc tgccgggcga tctcgcccag cgaaagatcc tcgtcgaaat gaagctgcat
      721 ggcctgaagc tgcttgtccg nnnnnnnnnn aaaaccccgc cgtaaaaggc gcacagggtg
      781 cccatttcca cgctggcttc cagccgggct tcctgcgggt tggggacgga cggttccttc
      841 aaggcgacgg ctcctttccc gctgtcaatg ctttttcctt gacagccaga tgattatagc
      901 cgaaaaatcg ctttctgtca acgaaaaaat cccgcaaaac ggagtttttc aggggactgg
      961 tcaatttccc ggaaggatgt tataatgata cattaggaaa agtacttttc accggcgaag
     1021 ggaggcggac ggatgccaat gggaaaaggc ctgcggcggg cagtctgcgc cctctgcctg
     1081 ctggcgctgg gcttcagcgc tttgccgctg tatggaatga ccgggtataa ccacccttat
     1141 tatgacgatt tcgggttttc cgccgcgccc catcaggtgt ggaaggagac cggcgatttg
     1201 ggcgcggccc tgaaagcagc gttgaaaaac gccgctcaga cccgctacac gtggcagggc
     1261 acctacacgg gcaccctttt gagcaatttg cagccgggta ccttttcgga ggagctgtac
     1321 tggatctccg gctgcttcct gctgacggcc tttctcggat gcttctggtt ctttttcgcc
     1381 acggtgtttg gccggcgggg gctgggactg atgcgctggg agaccaccgt tctttcttcc
     1441 ctgacgctga cggtgatgct ccagttcatg cccgatatgg gcgaggcgtt ctactggttc
     1501 aacggcggcg taggcaatgt gttcatttat tcgctgatgg cgctgagcgc ggcgctgctt
     1561 gtgcggctgt accggacgga aagccggggc ggcgctgcgg ggctgacggc cgttcttctt
     1621 ttgtgcatgg ccgccttggg cggcggaagc tacagcggcg gactgttcag cctgtgtgcg
     1681 ctggcctgcg tgctggcgtg gctgtttggg aagcgccgcc cccggcgctg gcggttcctt
     1741 ttgtgctttg ccgtgctggc ggcgggattc gtttacagca tgtccgcgcc gggcaacacg
     1801 gtgcgcgccg ggatgatcgg ttacacctgc ccgccggcga aggcggtggc ccgttctctg
     1861 tacgagggcg tgattttgat ggcgggctat gtgcgttttc ccctgctggg gctaacggcg
     1921 ctggtttcgc cccttctgtg gcgggcggcc aaggatagcc cctacac
//
LOCUS       DAWAEA010000263         1964 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7794_length_1964_cov_2.053431, whole genome shotgun sequence.
ACCESSION   DAWAEA010000263 DAWAEA010000000
VERSION     DAWAEA010000263.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7794_length_1964_cov_2.053431"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1260..1384
                     /estimated_length=125
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          357 a          571 c          536 g          375 t
ORIGIN      
        1 ggtaaatatc gctggtcttg agcagctcgt catgggtgcc cagcgccgct acccggccct
       61 gatccagcac cacgatctgg tcggcctcca tcacgctgcc cacgcgctgg gcaatgatga
      121 tcttggtcat gtcgcccagc tcatccttca gcgccttgcg gatcagcgcg tccgtagccg
      181 tgtccacggc gctggtggag tcgtccagca ccagcacctt gggctttttc agcaaagccc
      241 gggcgatgca gaggcgctgc ttctgaccgc cggacacgtt ggtgccgccc tgctcgatgt
      301 gggtctcgta cttatccggg aagccctgaa taaagccgtc cgcctgcgcc agctcgcagg
      361 catggcagat ctcctcgtcg gtggcgcaga ctgtccagat cgtagcggcg cacgtccacg
      421 ccgccgacct ccacgctgcc ctcgctgacg tcgtacagcc gggggatcat ctgcaccaga
      481 ctggatttgc tggagccggt gccgcccaaa atgccgatgg tctgaccgct ttggaaatgc
      541 aggttcacat gatccagcac cgggttctcc gagcggctgt tgtagcggaa gaccacgtcg
      601 ttgaagtcca cggagccgtc cttcacatcg gtgacggcgt tttccgggct gacgatgtcg
      661 ctctgctcgt ccagcacctg agcgatgcgc tcggcggaag cccgggagat gacgatcatg
      721 acaaagacca tgctcagcat catctggaca aaatctgcat ggtgtaggtg aacaggctgg
      781 tcagttcgcc ggtggacagg cccagcgccg catcgccgcc gcaggccacc accgccttgg
      841 cgcccagcca ggaaagcagc agcatacagc cgtagatgca gaactgcatc aggggcatat
      901 taaaggccag cagcttttcc gccttgctga agtcctgata tatctcttct gaaatttcat
      961 cgaacttctg aatttcgtgc tcctcccgca cgaaggactt gaccacgcgg atgccgttca
     1021 ggttttcctg caccacgttg ttcagacgat cgtagcgctt gaagacccgc tcaaagatgg
     1081 gatgcgcgtg ggaggccacc agatacagcc ccacgcccag cacggggatg aaaatggcga
     1141 aaatgccgga cagcttcgcg tcgatgccga tggccgccac cagcgagaag atcagcatga
     1201 agggggaacg caccgccgtg cggatgatca tctggtaggc gttctgcacg ttgctcacgn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnncagcag caccagcccc gccttccaga tatagggcat actgccctga gcgatgccct
     1441 tgtcgatcag gctggccatg atcagcggaa tgatgcattc catgaccact tccagcgaga
     1501 cgtacacggg cgctttgatg gacgcgggct tatattcccg cacgcttttg agtaactttc
     1561 gaatcatcgt gtcaatccct ccacgttcct ttctccattc tttcgtaaac ggtacagatg
     1621 gtatggccgt agcttcggat ttccagcagg acgcgccagt tccgggggac ggtcacgccg
     1681 tagatacggc cgtaagtggt attcatcaac agataggacg cgtcctccgg ctgctccgtg
     1741 acagtcagaa gggctttttc ctccgccgtc agggcggaaa gggcctgttc cagccccagt
     1801 tggctcagat cgtccatgcc ggacacggtc aggggcaggg cttcgttccg ctcggagccc
     1861 agcagaagcc gcagggcgtt gaccgtgctc acgtcccagt agtccagttc catggtctcc
     1921 tccgcgtccg caggggccag gaagttgtag taggcgtact gata
//
LOCUS       DAWAEA010000264         1960 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7817_length_1960_cov_2.362205, whole genome shotgun sequence.
ACCESSION   DAWAEA010000264 DAWAEA010000000
VERSION     DAWAEA010000264.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7817_length_1960_cov_2.362205"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    878..887
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          439 a          548 c          574 g          389 t
ORIGIN      
        1 cggggcatta gcgcagttgg tagcgcacaa cactggcagt gttggggtca ggagttcgaa
       61 tctcctatgc tccaccaaca agaaaaccct tgagtcgaaa gacttgaggg ttttcttttg
      121 tgtcttgaaa atgatccttg ttttttatca tcggatcgtc attggcccat ttttggaaaa
      181 gcaataatat agtataaatg tcgaggccaa caacaatctc tatgccattc cttctgcccg
      241 caccgtaagt atgtttgtac aaaaaggtag cgaatattcg tgttttccct tataatgata
      301 attggctaga agaaacaaaa aggagaaccc catatgaacg ccgaccaact ggctcaatac
      361 ttcgaccata cccagctgcg cgcctatgcg acggacgctg attttgagac cctctgcgcc
      421 gaaagcagaa aatatcattt ccgaatggta gccatcaacc ccgcccccgt ccggctgtgc
      481 aaacggctgc tggccggtac ggaaatccat gtaggcgcgg ccatcggatt tccgctgggc
      541 cagtccaccc gcgagatcaa aaagctggaa gccgaaaacg cgctggaaaa cggcgcggac
      601 gagatcgact acgtggtcaa cctgacgcgc ctgaaagaaa aggactatac cttcatcgaa
      661 aaggaaatgg aagggatcgt ggagacctgc cgcgcgcgcg gagccatttc caaggtgatc
      721 ttcgaaaact gctacctgac gcaggaagaa aaaatcgcgc tgtgcgatat cgcccgcagc
      781 atccgccccg actttgtcaa gacctcgacg ggcttcggca ccggcggcgc caccgaggag
      841 gacgttcggc tgatggtgga gcgcacccgc ggcgaggnnn nnnnnnnacg agtacgtcgg
      901 cacgggcgtg agcgaaatcg gcggcgaggt gaaggtcaag gcggccggcg gtatccgcac
      961 gctggacgcc gctcttcgga tgatcgacct cggggtatcc cgaatcggtt ctacggccag
     1021 cgtcaaaatc atggaagaat ttctggcgcg ttgaccccga aaagttaaaa tatggaaagc
     1081 gcccccgcag gcaaatccct gcgggggcgt atcgtttttt cttatcgggc cagccgggtg
     1141 aacaggtctt cgaaaagctc gtcgcggcgg atcatataga catagcggca cagcggacgg
     1201 cggaagtcct cgctgtaatg gtgatcgtac tggggaatgg gcgtgggaat cagcctgtcc
     1261 gccatgaact tcccctcccg attcaacagc cagccgatgg tggtcacatc ccagattacc
     1321 cggctccaga cgtgcccctt cgcataagcg tttgcggcgt cctccgtctg ctgcaccaga
     1381 taatcgcaca ggggattttt gccccggagc cagtattcca gctcgggccc ggtggtggtg
     1441 aaatgcgtga ccacgccgtg gcagggcagc agcaccagcg gcacgccgga gtcgaacgcc
     1501 acccgggcgg cggctacatc ctgcaggatg ttgaattcct gattatccgg ccaatgcagg
     1561 gcgctgccgc ccagccagac cagcaccatg cggtcggcta tctccggctg catcagcagg
     1621 gcggacgcta cgttggtgat ggccccgatc gccgccacgt acaggggatt ttcagtggtg
     1681 tgctccatgg ccagctgtgc cagcgcctgt gccgcatcgg atggaacggg cgttttttca
     1741 tcgggcagat aggtggtgga gccccggtag accggcacgt tttccccggc cagcgacagc
     1801 agcttcaaaa tttcctgata gcttttctcc atgccgtcgg cagggccggt ggaacgctca
     1861 ttatgaaagg gcgcggcgca cagcgccagc agatgcagcc ggtcctgcgc gcgcagggcg
     1921 taggaaatgg ctaactggtc gtcgatttcg ttataagcgt
//
LOCUS       DAWAEA010000265         1960 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7818_length_1960_cov_1.410499, whole genome shotgun sequence.
ACCESSION   DAWAEA010000265 DAWAEA010000000
VERSION     DAWAEA010000265.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7818_length_1960_cov_1.410499"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    947..1006
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1348..1455
                     /estimated_length=108
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          378 a          489 c          531 g          394 t
ORIGIN      
        1 catgatcgct ttctgatttc tgttccgcaa acaaagaccg ccgcagaaag cagaactgca
       61 gcgatcctgc agccgttctc gccgtatacc gggcagacgg aggccgtttt ctgtccgaac
      121 aggctgcatt ccatcgtctg gcactcgagg gcgccttttt tgccgcagcc cttgcgctac
      181 caactgagcc atacgaccca ttcattgtga gccgtcttcc gacgacgttg ccagtcatca
      241 taaaggcttt tggaaaagtc aagcataaaa cgggcatccg aaaatgtttc ttgaaaaagg
      301 gaaaaggcca gcgcggcggt ctcgtttgtg gcggcgcaga tggacaacgc caccagcact
      361 tcgtcgctgt gcagccgggg attgtggttg cccaaatagt cgcacttgag ggaccgaagc
      421 ggactgatgg cggtggggga gatcagacgg atcttcttgt tgatgccggc cagttttttc
      481 agcgcgttca gaagcactgc ggcggacggg cccagcaggc tggaggtctt gccggtgaca
      541 atggtgccgt ccggcagctc cagcgccagc gcgggggcgc tggtctcctc ggcccgctga
      601 cgggcagcgg cgatcacagg acgcatttcc gggttcagcc ccgccttctg atagatcatt
      661 ttcagcttgt ctacttcgct ctgctccgtg tgaccctcca ccagcaggca gcgggccgca
      721 aagtagcggc ggatgatctc ctgatgggcg gcttcccggc agacttcgtc gtcaatgatg
      781 cagttgcccg ccatatttac gcccatgtcc gtgggggatt tgtagggcga agtgccgtaa
      841 atgcgctcga atacggcctt cagcacgggg aagatctcca cgtcgcggtt gtagttgacg
      901 gtggtctcac cgtaggattc cagatggaaa gggtcgatca tgttcannnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngctg ccggggccgg
     1021 gagccgtcac caccaccagc ggacgggtcg tttctacata ttcattgcgg ccgaaaccgt
     1081 tgtcgctgac gatctgttcc acatcgtagg gatagccctc aatggtaaaa tggcggaata
     1141 cccgcactcc aaggttttcc agccgctgct tgaacaggac tgcgttgtgc tggtcgttcc
     1201 agcgggtcat gaccacgctg ctcacgtaca ggccgatgcc gcggaacgcg tcgatcagcc
     1261 gcagcacgtc cgcgtcgtag gtaataccaa ggtcgccccg taccttgctt ttttcaatat
     1321 catccgcgtt gatgacaatg accagttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnccctg gaagcgtgat agtcgtcaaa gagcttgccg ccgaattcca
     1501 tgtagagctt tccgccgaaa gtgtcgatgc gctggcggat acgctcggac tgcatggccg
     1561 tgtatttgtc attgtcaaac ccgatctgca tatgtatctt tcccctttcc agtgtacaac
     1621 ccatcttatc atgacatttt tcggaagaca agggaaagat ttgacgaaaa cggagaaagt
     1681 ttccgggtgg atgggcggcg aaagaaatgc tataatgcag aaaaaaggaa agggagagat
     1741 tcctgtgcgc cttgcggcag gaaaaatgct tcgcctacat ctgccgggtg ctgcaggtga
     1801 aaccggaccg gaaactgcag tatttccttt gcgaaacgcc ggaggaggta ggggcgtatt
     1861 acggcgatga ggaaccgtgc gacgggtttg cccgggaacc cgaccgaatc tatgccgtgt
     1921 acaatgagcg ctgccgctgc gacgggtttc atgaggacgc
//
LOCUS       DAWAEA010000266         1957 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7824_length_1957_cov_2.961094, whole genome shotgun sequence.
ACCESSION   DAWAEA010000266 DAWAEA010000000
VERSION     DAWAEA010000266.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7824_length_1957_cov_2.961094"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          422 a          583 c          578 g          374 t
ORIGIN      
        1 aatagaaaag accgccgtcg ggcgacaggc cctccggctc gcataaaaaa gccagcgtgg
       61 atttgaaccc acgctggtct tttgcgtttc aaaaatatcc aagctacatc gaagcatccc
      121 aagcaccgcc atttgagtct gcccagcgcc agctgcgggg gagcagagcg gcgataggca
      181 gcggagggaa aaaggcccgc ccattttgac ggcggaaaaa ggcgagcaca ggagcgcgca
      241 taagcgtagg agttatctgt ccaactaaat tgcgcgcgac gttgcgagcc ccgccggcaa
      301 aatgggcaat agaagccttt ttccggcggg tgagcgcagc gagccgaggc cgaggacagc
      361 cccgaagggg ctaccgcagg ccgaagccca cggcgcatag cgacgtgggc cgccttcgga
      421 gtcgccgtgc cgcgtgcttt ctttggtact ttctttcgct tcgaaagaaa gtactgcccc
      481 catcggggcg gcagccccac cgtccgcgcc gtagtcggcg cgtcaagccc ccggcagaaa
      541 aatgggctgc gcctgcgggc gcaccaaagg gctttccgct cgccctttgg aaaccttcgg
      601 ataccgaagt tggcgagaag cccacaggac ggtaagtccc aattcgcgcc gaagtcggcg
      661 cgtcaatccc tacataggag ccgttctccc ggcgtagctg ctgcgcactg atgcaatcag
      721 gggggcacgc ttccacgatt gctccagcaa aaacaacatc ccccacctca cgcaaaattc
      781 cccgcctcgc gctgcaagat ttctcccccc tccatcgtcc tatggatgaa ttcccaaatt
      841 ccggaaaggg aggaatcccc catgaaacgc cttgccctgc tgctgtccct cctgttgtgc
      901 gtcctcgctt tgccaagcgc ctgcgccgca cagggagaat ccgaactgct tgccgcctgt
      961 ctggggcagc gctacccggg cttcacggaa gccgcctacg cggaatgggg caacaccgct
     1021 gcggcggcgg tatttgtcca taacggccaa aagatcctgt gcctgttgga gaataccaac
     1081 ggccagtggc agatcaccgt ggataacccc aacgcccttg accagaccaa agaactgcct
     1141 tcgctcctgc tggacagcga tcagtccctt tattggagct atgcggagta ccccgagacc
     1201 acctattcca gctaccggga gacgggcggc gtttgggctg ccccgacgga gcaggtcgtt
     1261 tcctgggccg ggaaagaata tatcgtggaa gatgtttttt atcaggatgg ctggatcaag
     1321 catctgtgga gactggagga cgaagaaggc aatttgatcg agcagacggt ggatccttct
     1381 ttcccggccc cgtggctggc ggatatgacc accctgcagg ccttcgacct gtcccgcttc
     1441 ccggtgctgg gatataccga atacgagggg gaatggcccg accgaacctt tctcgcgcag
     1501 gccgccgcag cgctgatgcc gaatgatacc ttcctcagcg gctcttttgc ctatggatat
     1561 cttcgttttt tgatgcggaa atcggacgga accaaggttt tcgtgggcgt ggatgacgac
     1621 gaaggccacc ttcggcagga cgcgtactac ggggtggaaa acttcactaa ttcgctgggc
     1681 tggtttgagg atgcgtgcgt gacgctggat aaataccgtg cggaggacag ccgttggggc
     1741 gttatcagcg tggaaggcgg cgcgctttcc tttggcccgc agtgtatgag aagcgatgaa
     1801 aactacatgc tgctctattt cggttcccat ccgtggaatg atatgacgaa gatcgactgg
     1861 acgacactgc caaaaacctt ggaggaagcg gcggactgca tgaacggcag cggctatgcc
     1921 atggtgaaaa atcccgatcc cgccgaccgc cttcacc
//
LOCUS       DAWAEA010000267         1955 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7833_length_1955_cov_2.782105, whole genome shotgun sequence.
ACCESSION   DAWAEA010000267 DAWAEA010000000
VERSION     DAWAEA010000267.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7833_length_1955_cov_2.782105"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          420 a          614 c          575 g          346 t
ORIGIN      
        1 atacacgccc cattcgccga tgagcagggg cagcagcaga gcgttgccga tacgcatcac
       61 cagctccacc acgccggaga tcatgggggt cacggtgtcg cctagtccct gcagggtgga
      121 gcggtagacg aacagcaggt acagcatgaa cagccccgcg ctggccacat acagatagcg
      181 gcagcccacg gtgagcacct gctccactag cgcgggatcg tcgttgatga acagggacag
      241 gacagagcgc ccgaacagga cgaccagcag cgcgatcacc agcgccatgc ctacgccgat
      301 ctgggcggaa cggcgcagcc ccagccgcac ccgatccagc tttttcgccc ccagattttg
      361 cccggcaaag gtgctgaccg cgctgccaag ggagttgccc gccagctcga tcaggcccgt
      421 cagccgggtg gccgcattga agcccgccat gaagataaag ccgaaaccgt tgaccacccg
      481 ctgcagcacc agcccaccga tggagatgat caggttttgg aaggcaattg gcacgcccag
      541 ggccatcaat ttgcgcatca ttcggggatc gggtctcaaa tcggcccgtg ttatccgaat
      601 gaccggcatt ttccgcagcg ccagcaggca gatcaggctg ctgagacatt gggacatcac
      661 cgtggcgatg gaaacgccgt tcacgcccca acggaagacc gccacgaaca gaatgtccag
      721 cccgatgttg caaagggcgg cggtggtcat ggcgatcagc ggcgtgcggc tgtcgcccag
      781 cgaacgcaga aagccggaca gaaggttaaa cgacatcaca aataccacgc cgccgaagat
      841 gacccgcagg tattggcagg tcagggcgat ggtgttttcc ggcgaacgca gcgctgtcag
      901 catgggccag agcagcagct cggacagcag cgtcagcccc acggtgatgg ccgcggaaag
      961 aagaatgctc tgcccggcag cccgccgcag cccggcataa tctccggccc cgaaggattg
     1021 ggcgatctgg atggaaaagc cctgcgccat gcccatggcc agccccagca cggtccagtc
     1081 cagccacccc gccgccgaaa ccgctgccag cgccgttacg ccctccacct gtccaatgac
     1141 cagcgtgtcc accagactgt aaaactgctg aaatagattg cccgcaatga tgggcagaca
     1201 aaaggacagg atcagcttcg tcggattgcc tcgcgtcatg tcccgggttc gtgccgccat
     1261 ggtgtgccct ccccaaaagt aaaaatgaaa cgtgactaca ttcgcttccc gccgcccaat
     1321 tcctgccagg tggatgaaaa tagttttgca ttctgaggtt cttttgggga tggagcagct
     1381 tttgattgct tttgtgcaaa gggtagagtg ggcacaggaa ctcctgaagg aaaattgtgg
     1441 cgagcggaaa gccctttggt gcgcccgcag gcgcataccc ctgccccggg ggatgacgcg
     1501 ccgactacgg cacgggagca gggcttgcag ctctatgggg cgttcacact tttctttcga
     1561 tggcgaaaga aaagtgtgca aaagaaagcc agcggcacgg cgactccaga ggcggcccgc
     1621 cccgcttcgc gtaattcgca aatatcggcg ttccgggcac tgccgtgccc ggcccttgta
     1681 tttgctcagg cctgtcagtc gctaactgcc ttcggcagtt gtgcccactg ggcacacgct
     1741 ccctctcggc ccaagcttcg gcctgcggta gccccttcgg ggctgtcctc gtcctcggtt
     1801 cgcttcgctc acccgccggg aaaaagccct ttattgccca tttttgaaag agggagttcg
     1861 caatgtcgcg cgcagcacaa ttggactgcc aacatgaacg ttcgtgcgcg ctcgtgctca
     1921 ctcttttcct ctttcaaaaa gggcaaggct ttttc
//
LOCUS       DAWAEA010000268         1954 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7839_length_1954_cov_1.378620, whole genome shotgun sequence.
ACCESSION   DAWAEA010000268 DAWAEA010000000
VERSION     DAWAEA010000268.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7839_length_1954_cov_1.378620"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    408..417
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    746..755
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          408 a          539 c          608 g          379 t
ORIGIN      
        1 cggcggcgtg agcatcttcg acaatcccta tttgaacgaa gaagacattt cgcccgacga
       61 ggctctgggc gaggaaaact acatcgaccc ctatgacacc ggcgcggaca cgggatcgct
      121 gctgagcgcc agctgtgctt ccggcggtgt ggagaccgtt tacccctacg cgggcgcgac
      181 gccgatcccc ctcgacccgg tggatctgcc gacccccacg cctcatccgg agctgacctt
      241 taattacgcc acctacaccg ctggttctct gggcctgtcc tttgactgcc ccagcggctg
      301 gctggtggac gaatctcaga cggacgtttt tgtcctgact gagccggaaa cgcagatgca
      361 tgacggacag cagtgcatta tcaccatttc cgcagagcct gtcaccgnnn nnnnnnncta
      421 cagcgagaag gatctgaaaa agcaggtcac ccagcgcctg aaggaaattg gttccgtaaa
      481 cttcaccgtc tggaatccca gcctgacggc tacgcgcacg ctgttggatt ccaagggtgt
      541 ttatgccaac tacacgggca ctctggccaa cggggtggaa ctggcaggcc gaatcctgta
      601 cgccagcacg aacaacaagc tctatggcgt agagatggtc tatccccggg actaccggga
      661 cgactacctg aatattttcg ccaagatccg cgaaaccatc tctgctcaat aaggcaggca
      721 gggctgaagc cctgcacccc acaccnnnnn nnnnncttga ggggaagagg gctggccggg
      781 gttttttacc gccgtggcac ggaaggcacc acggctcgct ggtttggtag ggtaaaagtt
      841 ctgcgccttg gattatgggc gatgtgcgca ggggcgtagg ggaacgcgca ggttgtctgc
      901 gggaatacat tacatgaaaa aaagagacgg gagctgctga cggcggctcc tggtttttga
      961 tggggagatg aagaaaggca aatcggaaaa gtctcatggg agggacaaaa gtgcaaggaa
     1021 tgctcgattg tactgtttct gacacggatg gatattgacg cgtctagaaa agcagggtat
     1081 tctttggata caatctgttc tttccgccag cggggcggaa gacgcggaaa agcacatgca
     1141 gcatcaagga ggaatccctt tcatgaagcg atccagcctt tggcaggaca tgacggtact
     1201 gcatcagaac cgtcttcccg gaagaaccac gtcgtttcct tttcccgatg aagccgccgc
     1261 cctgcggggg gaacggagcc tgtcggacgg gtatctgtgt ctgaacggcg tctggcagtt
     1321 ttactatgcc gccgacggcg tgctgcccaa gggaatctgt cagggcgggg aagaagaaaa
     1381 cgtgctgtgg gacgagttgg acgtgccctc ctgctggcag atgcacggct acgggcggcc
     1441 ccagtatgtg aatgtcagct accccattcc ctttgacccg ccctatgtgc cggacgaaaa
     1501 tcccacgggc gtataccgcc gggttttccg cctgccggaa agctggcggg gacgccgggt
     1561 gacgctgaat tttgacggcg tgaacgcctg cttcttcgta tgggtcaatg gccgggaagt
     1621 cggcatgtcc aagggcagcc gtctgcctgc ggagtttgac gttaccgact gcctgaagga
     1681 cggcgacaac accctgaccg tgcaggtgct gcaatgggcc gacagcacct atccgggacg
     1741 tggtctgccg ggtggatctg gaaaaccatt accgggacgg cgtgctggac gtggaggcgg
     1801 atgtgcccgc cggatgcgaa gccgtcgccg cgctgtatca gggggaaagg aagctcgccg
     1861 aagcggcgct ggttcagggc cgtgcccgct tctccgtgaa ggactgtctg cgctggacgg
     1921 cggaaacgcc tcacctgtat accctgctgg tgtc
//
LOCUS       DAWAEA010000269         1954 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7837_length_1954_cov_1.622433, whole genome shotgun sequence.
ACCESSION   DAWAEA010000269 DAWAEA010000000
VERSION     DAWAEA010000269.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7837_length_1954_cov_1.622433"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1501..1510
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          336 a          615 c          543 g          450 t
ORIGIN      
        1 tccggggcca acaacagcgg aatggccccc gcctccgtca gagaacgcac atagtcccga
       61 ttgatatgat attgattttc ctccgtattc atgctgcctg aaatgccaat caatggctga
      121 cgatgcatga aaaccccgcc cttccttttc ttttgttccg aatttagctt atgctgcccc
      181 attcgatctt ttcaaaatac gcggcgtttt gccgcccatt ttcttatttt tcgcagttgt
      241 tcacgccgtt ctgaacggct tcttcttcca cttcccgctc cgcgtcccgg agatcgtcct
      301 ccgccagctg cccgccctcg gctgcccgaa gatcgtccgc cgtcagggtc atcagcattt
      361 cccgggtaac gtccggcagc gccgccagcc ggttggcctg tgccgccacg gtacgctcaa
      421 acagattgcg cacgccgcgg gcattgccga agcccttcac gtccagactc aacagtgcca
      481 gcagactgcg taaaagtccg cgagcgtcct ctgccagctc gtagccgctc ttgcggcagc
      541 gcatatcgaa aatatccatc atttcctgca ccgtatagtc cgggaaattt aagaaacgat
      601 tgaaacggct ttccagaccg ggattggaat gaacaaattc ctccatcagc tccgtatagc
      661 ccgccacgat cacgatcagg tcgtcccggt gatcctccat ggccttcagc agggtgtcca
      721 ccgcctcctg cccatagtca ttgccgcccc ggttggtcag ggcgtaagcc tcgtcgataa
      781 acaaaacgcc gcccatggcc ttctgaatgg cctcaccggt cttgatggcg gtctgaccca
      841 catagcccgc taccagcccg cttcggtcca cctccaccag atgccccttg ctgagaatac
      901 ccaagctctt gtaaatgcgg gccatcagcc gggccaccat ggtcttgccc gtgccgggat
      961 tgcccgaaaa caccatatgc agggacagat cattcaccgg caggccgttt tccttgcgca
     1021 ttttctgcac cgtcaccaga ttgatgaggc tttccacctc ttttttgacg gtgtcaaggc
     1081 cgatgtactc gttcagttct tttttcagat cctcgatgtt ctccggcggt tcctcctcaa
     1141 cctgttccgt tgctgcagga gtctgctgcg ttttcttttc ttcctcggct ttcccggcgg
     1201 gagccgcggg aacgtctgtc gtttccgcag agggcgaggg cacttgcggc gcagacggta
     1261 cgccccacag atcgtccgcg atccgattca ggttgttccg ggtcatctga ttgataacgt
     1321 ccatttgcag acggatgatt tcgtctctgc gatccatggt atttattcct ccatcagcgc
     1381 cgtccggggg atgaacgcac ggcacggctg attttctatc gtagtttcca cataggccca
     1441 ttcgctgttc aggaaggcca gcagcgtcac gctgtcccct gtccgcaggg cggcgagggc
     1501 nnnnnnnnnn gatttagccg atgcggccct gctggccgtt tccgatggaa tagctgacca
     1561 gtgcccagcc gtcctccgtt acgccgtaaa cctccagatt ttcatccgtg tccgtttggg
     1621 cgttgcctac tcgataggcg ttttccgagg gagccgtgta gaccgcctcg gtctggcggc
     1681 ggcggaagga agcctccggc tggagggaca gaaggggcgc ggcggcctgc gcctgcatat
     1741 agggtgaaag cgtcggcgta ggcgcggcgg taggccccgg cgtgaccggc acgggcgtat
     1801 ccgtggggac gggggtgtcg gtcggcgcag gggtatccgt gggcgcaggg gtgggggtag
     1861 gaaccggcgt gggactgggc gtaacaaact ctgccggggc caccgcgtcg cccatgcggt
     1921 tcagcacgta ccccttgggg gcttcgccgt ccgt
//
LOCUS       DAWAEA010000270         1953 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7843_length_1953_cov_2.130137, whole genome shotgun sequence.
ACCESSION   DAWAEA010000270 DAWAEA010000000
VERSION     DAWAEA010000270.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7843_length_1953_cov_2.130137"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          375 a          542 c          701 g          335 t
ORIGIN      
        1 ccttttacct ggggagaaga aggactgcgg gaacgctgcg agggctaccg gcagcgcctg
       61 atggagccgg tgcttcggat gcgggaaggg ctggtgcagg cccgggacgc ggctgcgtcc
      121 atgacggcgg tgttcggcct tttgaaggat gtgggcgctt acgagcgcct gcagcgggag
      181 gaaactgagc ttttggaaaa aggactggct gttcgagcgg ggcagaacag tcaggtatgg
      241 cagacgacat tgactttgat ggatcagatg gtgcggctga gcgaccggca gaggatcccc
      301 ctcaagcaca tcgccacccg gatggagtgc ggattttccg cgatttcgct ggcggctctg
      361 cctccggcgg gaaaaatgct ccatgtgggc gtactgggtc acgctttgtc ggaaaacatg
      421 gaggccgtat tcctgctggg cctgaacgac ggcgtgctga cccgagagac acaaagcctg
      481 ttgagcccgg aggaacggca ggacgcacag gaaaagaccg gctgttttct gggcgtcacg
      541 gatgaaagcc ggaacctgtt cgccaaactg gacttgaagc gggcaatgac cctgccgtcg
      601 aagctgctgt ttttgagcta tgccaagacg tcggtggagg gcaaggcgct tcggcggctg
      661 aacctgctgg atacggtgga aaagcagctt ttaccgtcgc tgccccaatc cccggagccg
      721 gaggaaagtc tgcctcaatc ggcggtgcag gcgttggcgg agttgagtgt gcagctgcgg
      781 gcccatctgg acggaaaggg cggcagcgag ctgaccccgg acatggcgga gaagctgcgg
      841 aagctgctgc ggagccggga gaccgctcct gccacgctgg ggctgctgcg cgcgctgcgt
      901 tacgacggca gcgcgcagcc catcaccccg gagacggccc gggcgttgta cggcggggaa
      961 acgctgtccg tcagccggtt ggagcagttt gcgggatgcc ccttccagca tttcctgaac
     1021 tatgggcttc ggccgcaggt gcttcgggaa tggaaggtag accccatcga aacgggcaat
     1081 ttttatcacg cggcgctgga cgggttttcc cggctggccc ggcgggagaa gggctacccg
     1141 gcggtctcgc cggaacgggt gcggcagatg acggaggaag ccattgcgcc gctgtacgac
     1201 gaactgatga gtggccccat gggggacggc gaccgcagcc gcgcccggta cgatcaggcc
     1261 aaaaacgcgg tctgccgcgc cacggaggtc atcacccggc atctggcggc gggcagtttc
     1321 cggctggatc ggacggaagc cgccttcggt acggaaaagg gattgccgcc catcgtgctc
     1381 tgcctgccca ataggcggga gatcatgctg cggggtcgta tcgaccggat cgaccgctgg
     1441 gatcatgagg ggacggtcta tttccgggtg atcgactata aatcgtcgga acaggcgctg
     1501 gaagcggcca agacctggtg gggacttcag cttcagctgc tgctatatct ggaggtctgt
     1561 acggcttcgc tgcccggctc caagcccgcc ggggcgttct atttctatgt ggccgacccg
     1621 ctggtggaga ccgccaccga cgcggcggag gccgtggagg gcgaaatgcg caagctgttc
     1681 cggctgcggg gcatcaccct gtgtgatgtg gaaattctgg aagctatgga caaaggcgag
     1741 gaggccttcg tgctgccgcc tgtcctgcag aaaagcggcg aactgcgcaa ggacgcaaag
     1801 gcgctgacct tgccccagat gaaagccctg atggagcacg cccggcagac cgccgcggcg
     1861 ctggcgcagg aaatgatgga cggcaagacg gacatctgcc cggtacggga cggcggacag
     1921 gcctattgga accgatgcga ttaccagagc gta
//
LOCUS       DAWAEA010000271         1944 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7880_length_1944_cov_1.881419, whole genome shotgun sequence.
ACCESSION   DAWAEA010000271 DAWAEA010000000
VERSION     DAWAEA010000271.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7880_length_1944_cov_1.881419"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    902..911
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          427 a          559 c          591 g          357 t
ORIGIN      
        1 acgattgagg ttgcggatca ctgcccctgc cctgcccctt agcgggagcc cttgtcgtag
       61 gggatgcctt cggccttggg cgcacgggag ctcttggagg tgaaggccag aacgatcagc
      121 gtgatcagat acggcagaat ccgatagaag ttgatgttga tcccgatttc cttcagcagg
      181 aagatgccgt cgccattcag atccagactg gcgtagctgg ccgccacaca cttgaagaga
      241 ccgaagagca gcgccgcgcc cgccacgttc aggggcttcc agttgccgaa gatcatcacg
      301 gccaacgcca ggaagccgaa gcccgctacg tcgccgttgg cggagcagtt tgccgtggtc
      361 atggcgtaga caaagccgcc caagccagcc agcgcgccgg aaatggtcac gccagcgtag
      421 cgcatcttca ccacgttgat gcccagactg tccgccgcct gaggattttc accgcaggag
      481 cgcagccgca ggccaaagcg ggtcttgtac aggatcaccg ccagaatgac gtagatgaga
      541 atgcataggt aggtggccag ataaaccttg ttcaggaagg tctcggcaaa gaagcccagc
      601 ttatcggtct tggcgtaacc cagcatggtc tttttgatca tgaaccagct ggcggagtcg
      661 ccggtgtaca tctgcagggt gttctggttg gccagcaggc ggatgaagaa cagcaccaac
      721 gcgggggcca tcaggttcag ggccgtgccg ccgatggtct gatccgcctt caggttgatg
      781 gcggcaaagg ccagcagcag actgtacacc ataccggcca gtgccgccac cagaacagag
      841 atcagcacca cgaggaagac cggcatttcg gcaggcagca tggtcagggt gagaacaccc
      901 gnnnnnnnnn ngccatggcc agcacaataa acccttgcag cgccggccag tccttggcgg
      961 ccatggccgc gtcaaaccag ccccactgct gtaccagacg cacaaacaaa acgccgatga
     1021 acgcgccgaa gatcataatg ccctccagcg ccagattgat gatgccgctg cgctcggcaa
     1081 aaatgcccgc cagcgctacg atcatcaggg gaaccgcgta gatcagggtt tgacgaatca
     1141 gaaaactcat ttctccgccg cctccttccc atctccggca ggccggtcga tgttttcact
     1201 gttgttggtc tcctgggtcg cgggaatgat cttttcctcc cgatgacggc ggcgcttgcc
     1261 gctgccggac agccacatgc ggatcaccag cgagaacgcg gccagataca cgatcacggc
     1321 aatgatgacg tccgtgatgt attcgttgta cgcggtcagg ttggtcagct gcaggccggc
     1381 aatatccagc atggacatga acatgcccgc gaagatcaca ccgatgggat ggttggaagc
     1441 cagcagggct acagggatgc cgttgaagcc cacggcgggc agctgctggt aggtggacca
     1501 gaaaaactcc gtgttgccgc tgaggtagta cagggagccc gccgcgcccg ccagcgaacc
     1561 ggcgatagcc atcgacagca cgatgttccg cttgtccttg atgccggcat agcgcgccgc
     1621 atagcggttg gagccgcagg ccttgagctg ataaccgaag gtggtcttgg aaaggatgat
     1681 gtacacaatc accgccagca caatggcgat caggatgctg ccgttgacct gagagccggg
     1741 gaacagctgc tccagcccca ttttggaggt ggccacgccg ttggccgtgg tcttgtaaat
     1801 gtagctggtc ttggtgtttt caatgacgtt cttcaggttg gacacgtcaa agacccacgt
     1861 ggccagattg gcggcgatcc agttggtcat gatgcaggcg atgacctcgt tgatgttcag
     1921 cagagacttg agcagaccgg gaat
//
LOCUS       DAWAEA010000272         1943 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7883_length_1943_cov_2.578390, whole genome shotgun sequence.
ACCESSION   DAWAEA010000272 DAWAEA010000000
VERSION     DAWAEA010000272.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7883_length_1943_cov_2.578390"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    234..379
                     /estimated_length=146
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          330 a          600 c          531 g          336 t
ORIGIN      
        1 attgcgagcc ccgccgtcaa aatgggcaat aggagccttt ttcccggaag ggtgagcgaa
       61 gcgaaccaag gtcgaggaca gccccgaagg ggctaccaca gaccgaagcc cgcgacgcat
      121 agcggggcgg gccgcaagag tcgccgtgcc gcatgctttc tttggtactt tctttcgcgt
      181 cgaaagaaag tactaccccc ctcggggcgg cagcccctcc gttcgcgccg gagnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnt tgtatccact ggatacccgc ttcccttcgt tcccctttgg
      421 aagccttcgg ggcccggaaa gctggggagt ttatccccat ggggagacat ccccaccgcc
      481 ccgcgccgcc gtcggcgagg caggccgttt ctccctattc cccgtttttt tcgcttctct
      541 tcttccacgc cagccagtcc ttgtccagca cggtgacatg gggccgggtc atgatcgtgt
      601 cgcatggaaa atgctggcac tggccgcaga agtccagccc ccgctccttc acgcaatccc
      661 gggtaaagca gtctcccgta acatgggctt cctcgcagcc ggggcagccg cctttcaagt
      721 acgtgggaca ggcgccgcag taaaaaccgc atctgccgag taaactttca tccatcctcg
      781 cattctcctc cataaagacc cacaaacgtt tagatcacat actccggcgc agtcggcccc
      841 gtcatcaggt ccgccagcgt gattccgtca aaaaacgcgt tcgtccgtcg ggcgtattcc
      901 tgccacatgg gcagcgtacg gcaggaagcc gcccggggac agggcgcggg cccgcagctc
      961 aggcagctca ccggggccag atcgccctcc gtcagacgca gcacgtcccc cacacggatt
     1021 tcctcggcgg gacggcccag acggtagccg ccgcccttcc cggactgccc ctccagcaga
     1081 cgggcccggg ttagcagggg cacgatgcgc tccagatatt tcagggaaat ttcctgccgg
     1141 ttggcgacct ccttcatggg cagataggag ccgccgccat gctcggccag atcgaccaga
     1201 actctcaggg cataccgccc gcgggtggaa atcatacgaa aacctcgctt tgttcattgg
     1261 gacagaaaaa ggcggccccg gctttgaagc tgccggggct gtgccgcttt gggaaaactt
     1321 agtccgcaaa catcggcgtg gaaagatagc ggtcgccggt atcgggcaga aggacgacga
     1381 tatttttacc tgcgttctcc gggcgcttgg ccagctggat cgccgcccag accgccgcac
     1441 cggaggaaat gcccaccagc acgccttcgc tgcggccgat catgcggccg gtggcaaaag
     1501 cgtcctcgtt ggagacaggg atgatctcgt cataaacctt ggtgtccagc acttccggca
     1561 cgaagcccgc gccgatgccc tgaatcttgt gcgcgcccgc cacgccggtg gacagcaccg
     1621 cagaggtggc aggctccacg gccacgacct tcacgttagg cagcttggat ttcaggtact
     1681 ggcccacgcc ggtgatggtg ccgccggtgc ctacgcccgc tacaaagatg tctaccttgc
     1741 cgtcggtgtc ctcataaatt tccgggccgg tggtctcgaa atgcgccttg gggttggccg
     1801 cgttcacaaa ctggccgggc acgaagctgt tgggaatttc gtgggaaagc tcatccgcct
     1861 tggcgatggc gcccttcatg cccttggcgc cctcggtgag caccagctcc gcgccgtagg
     1921 ccttcatcag ctggcggcgc tcc
//
LOCUS       DAWAEA010000273         1940 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7894_length_1940_cov_2.349602, whole genome shotgun sequence.
ACCESSION   DAWAEA010000273 DAWAEA010000000
VERSION     DAWAEA010000273.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7894_length_1940_cov_2.349602"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          382 a          549 c          604 g          405 t
ORIGIN      
        1 ccagtagcct tcctcgtcat aggggaagcg caggttttcc ttgacggggg tgtcgtagct
       61 gtgcagccag ccgccttcct tcagctccag gctgtaggcg ggctcggcca ccagcatcat
      121 gtcgcagccc agcgtgccgg tttccatttc gccggccagc ttggtctgca gggagccggt
      181 gccgccatag aagaagaagg agccgtcgtt gcccggggtc aggttgggga attcggcctt
      241 cagggcttcg tccatcatat cgatgacgaa ctggtacatg gaggtgtaga tcataacggt
      301 gccggtcacg tcctcgttca cggccagcgc ggcgggagcg acgcacagga gcatggcgat
      361 gcacagtacc agagagacga ttttcttcat gagacaggtt cctcctttaa cttcgtgaag
      421 ttctattttt acattggcgt accaatgtag ggtacagtaa aaaagcgcgc gaaataaggg
      481 ggttatcgag ttgtatctca tccttttggc aattcctatt ttaccagaaa acttctccct
      541 tggcaagcaa aaaatgtctg aaacatatct atttttatcc aaaaacgccc ctttcagagt
      601 gaaaggggcg ttcagaagcc gttaagattt caggcgtttt ccagcgcctt tttgccaagg
      661 agataagcgc cccacaggcc gctgacgggg tacagggccg ggggaacgat gtagtcatcg
      721 gtatggttca gcagacggtc ggactggacg taaccgccca gcagacgcag ggtctcctgc
      781 cgcaccagcg ggaacaggcc ctcccgctgc atcacgccgc cgcccagaat gatcttttcg
      841 gggctgacgg tgacgatcag gttctggcac atctgggcca gataatgggc ctcgatcttg
      901 aaaacgggat gatcgtccgg cagctcccgg gcgtcgccct gcacccgcgc gccgatggcg
      961 gggccggcgg caaggccttc cagacagccg tcatgatagg ggcacacgcc gtggggaatg
     1021 gggtcgtcgg gatggggacg gagcagcata tgccccactt cggggtgcag cagaccgtga
     1081 acggtatggc cgcccaccac cacgccgccg ccgatgccgg tgccgacggt cacatagacc
     1141 acattgctgc agcccttggc cgcgcccatt tccagctcgg ccaacgcagc ggcgttcacg
     1201 tcggtgtcga tggcggcggg aatgctcttt ccctgcagca gttccggcag cagggggtaa
     1261 tccttccatt tcagcttggg ggtggaggtg atgtagccat aggtggggga atccgggttc
     1321 gggtccagcg ggccgaagct gcctacgccc agcgcgtcga tcttccagcg gtcgaaaaac
     1381 tgctgcatgg gcggcatggt caccgccggt tcttcggtgg ggatggtcat tcgttccagt
     1441 tccttgccgg tctcgtcaaa ggcggccagc accattttgg tgccgccggc ttccaatgcg
     1501 ccgatggtca tgtgcgttcc ttctttcctg atgataagcg ggaagcaatt taaaagttct
     1561 catcatgatt ttatcccaac cggggcattt tggcaaggga ggaagccggg caatatgatt
     1621 tctcattgcc cccatgggcg gtttgcgcta caatgaaggg gaaagtttta tcgtcattcg
     1681 gaaggacgga gccggaatga attttacccc tgaacagcaa agcgctatcg acgcgaaaaa
     1741 ccgagagctg ctggtatccg ccgcggcggg cagcggcaag accgccgttc tggtgcggcg
     1801 cattcttcat ttgatccggg acgagggcta ttccatcgac cggatgctgg tggtgaccta
     1861 tacccgcgcc gccgccgctg agctgcggga ccggctggag accgccatgc gggacgcggc
     1921 gctcagcgac cccgccatgg
//
LOCUS       DAWAEA010000274         1932 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7934_length_1932_cov_5.969100, whole genome shotgun sequence.
ACCESSION   DAWAEA010000274 DAWAEA010000000
VERSION     DAWAEA010000274.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7934_length_1932_cov_5.969100"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          448 a          517 c          633 g          334 t
ORIGIN      
        1 aaatcgaggc caaaaactga ataaaaggtc ttgcgaagtc gtgtggctgt cgctgtaaag
       61 gcggtatatc attaccgtca ggcggcagtt ttgaaggaac ggggtgatac cccctgcgga
      121 cggcgctgtt gacgtaagga atggaccaaa gcggcagaaa tgccgtgtgg cgaatagcag
      181 ccattggagc gaacgctctg agaaggtagg ttcaggtacg agaagggcaa aagcccttcg
      241 tgacgttagg gcagaagaat tgcacgatgg aggatcagtg ttgtacagcg ggcgatctct
      301 ccctgacggc ataggcggga gtgcgcgctg gcgagattcg tcagcgcatt ttcatttctg
      361 ccggatcacc gctggttttc tttggaaaac cggcgttttt ttgtaaggga ggatcacgtc
      421 tatgtccaac aggaatcccc gcgagagatt tgactacaag agttggtttg agcgtaatgt
      481 ggccaagcgt accgtggcgg cgctggaagc gcgggacgcg gagtttgccg aaaagcacgc
      541 ggatacgccg ctggcgcatt tggcgcaata cgtcggcaat cgtgctgaac tgctgcggca
      601 cacgcccagt ccctgcgaga tagacggcgg cacgtttatc gaggagcgct tcggcggttg
      661 gaccgcagta ttggagatgg cacacctgcg cccaccaacg aaagagccca agttgaaaga
      721 cacggcacgc tacaagcggg aaaaagccgt gcaggagccg ctgttctacg aggaatccga
      781 gcgcaagaag aaggcgaaac gcgccaaggc cgcagccagt catgcggcgc agcaatcacg
      841 gctgaaagag aaaaagcggc tggagcagga aaagaaagag gctcgtgatg ccgctgcccg
      901 ccagcgcgaa attgagaaag cggcaaaggc cgccgagcag gaggtgtcct gctgagttac
      961 ccttgtcaag ccgagacagt ctgaacggag actgtcagct ttccataagg gtgggagggg
     1021 aaaagccgcc cctcccacct cctctctttg ttgaacggca agaccaaaac cacatacaaa
     1081 agccaatatg ttacccgccc ctgcggggca ggtaacggga aaaatacgcc ccatctgcgg
     1141 ggcgagaaac aggaatagga gcaaacacac atgatctctt tgagtgaaat actgcatacc
     1201 atcgcccagg cgctgatgat cccctgcctc attatactga taattctgat ggccggggcg
     1261 gtctggcaga tcggcgacat cgtggttgag tacatcgcgg agcgccgcaa gcataagtgt
     1321 aacgtccccc agctgctgcg cgacgtacac gcggcgggcg cggacggcct tgcggagctg
     1381 atcgaaacca gcggcctgct gcggcggcag aagaaggccc tgctggagct ggcggagagc
     1441 cgcggcctgc cgaaggacac cctgacggca ctggcggagc gtctgctggc cacggaggaa
     1501 gcgcggaacg cccgcaccac ctccatcacg gacatgatcg ccaagctggg ccccatgttc
     1561 ggcctgctgg gcacgctgat acccctgggc cccggcatcg tggcgctggg acagggcgac
     1621 acagtgacgc tgtcggagtc catgaacgtg gcattcgaca ccaccatcgc cggcgtcatc
     1681 agcgcggcgg tggccagcgt catctcccac atccgcaagc ggtggtacaa cgacgacatg
     1741 gtgtcgctgg aaacgctgat ggaggcagtt ttggaggagg tcacgagcga tgttgaaggg
     1801 taagttttcc tcccgccgcc gggtgacgga ggaggtggac gccatgtccg ggctggcgaa
     1861 cctgtcggac gtgatgctgg tgttcgcctg cgggctgatg gcggcggtca tactgaactg
     1921 gaacgtggat ct
//
LOCUS       DAWAEA010000275         1929 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7952_length_1929_cov_1.778015, whole genome shotgun sequence.
ACCESSION   DAWAEA010000275 DAWAEA010000000
VERSION     DAWAEA010000275.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7952_length_1929_cov_1.778015"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    222..379
                     /estimated_length=158
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1310..1476
                     /estimated_length=167
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          429 a          464 c          378 g          333 t
ORIGIN      
        1 ggcctctatg gtggaagata gagacgaacg gcagcacgca ctggaactgt tgtgccgcaa
       61 gtatgcacca gagcaggggg cagaaatttc tcctgccgca gcccgtggtg ccgaaaatac
      121 ggctatcctg cgtattactt tgacagaaat tcatggtaaa cgcaaagata cgtaatatct
      181 ccgaatgaaa aataaaaacc ggaacaatca cgtaaagatt gnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnna gctaatacag gcaatgcacg cggccgctag cagcgaaaat
      421 gttagggaat ccaaaaaagt cgcatgccag cgttcctgaa tgagcagaaa cagaatgagg
      481 aacaccacaa agaggataag cagcgaaacc gggtcgctca gtccacgaaa aatcgtcaca
      541 tgcacatgag tgcaaccgtc tttttccgtc aaaaatccca tcgctttgtt atacgaccag
      601 cgtccccgtt cagccgcctc tttgtcgcag gtgtaggcca tactgaaaaa gcggcctttc
      661 acccacccgg tatagcgttc cgccttaaaa attttgcggc tgtccagcgc gtctttcagc
      721 gcctgctggc aagcttccat aggctgatcg gtttgaatat gcatacgccg ctcctttcct
      781 tagtgccggc cggcaccacc accgcggctt gagccaccgc cgccatggaa tccgccgcca
      841 ccaaaaccgc cgccgcggaa accacctcca agaccgccgc cgattccgcc gccaaaaggc
      901 ggacgaggac cgcggggtgg acgcggggga cgcggaccac gcgggggacg tggccctcta
      961 ggaccacgcg ggccatgagg ccaaataaac ggaaagaaga aaaaggaagg accacggcga
     1021 tatcctccgc cgcctgctcg ccgcaaattg gaaagaatca cggccagaac caccacaaag
     1081 ataacaacga atacggtaaa tcctattgtt accataccag ggttatctgc atagtcatac
     1141 tggtattcat agtaatactc atcttccggc tgataggtac cggcatagat actttccacc
     1201 cggtcataaa gttgggcaaa tgttttctgc acggcggcat catactgccc ggcggcaaag
     1261 tcgggttcca tatacgtatc gagcaggttt tgaatggttc cgctggtaan nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngccc tgtcccttgc aacacatagt
     1501 aattgtcttc gccaatcgcc agcagaatca ggatgccatt attacgatct ttatcgccaa
     1561 tgccccagct attaaatagg tcataggcat aatcatcgat agcctgtccg tccaaaaaat
     1621 ccaccgtcac cacacagatc tgcgcaccgg tctgctggta cagcgtctga ttttgagaaa
     1681 caatggtatt ttctgtgctg ctatccaata cattggcctg atcgagcaca taaaactggc
     1741 tgccaggttc aggaacggcc gcctgcaccc caggcgccag ccctaccagc atgaccgcca
     1801 cacacactac aatggccgtc agccaccctg ctttacgcag cgcttgtctg ccaatttgtt
     1861 tcatattttt tcctttctat cccaccccgc atgttacggg gccgcaatct acaaaaatag
     1921 ttccaactc
//
LOCUS       DAWAEA010000276         1927 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7959_length_1927_cov_1.835470, whole genome shotgun sequence.
ACCESSION   DAWAEA010000276 DAWAEA010000000
VERSION     DAWAEA010000276.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7959_length_1927_cov_1.835470"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          412 a          537 c          581 g          397 t
ORIGIN      
        1 tttcgtgcga ccattatttc ttcataaaga aataatgaat acaatttata ttatgttcat
       61 gatataaatt gtattcatat tatattatat atttcagctt cgtgtcaagc tatgatgaca
      121 gcatgaggtg atgacatgaa acgtgaaatt cggatcacga aaaagaaggg ggaagacgga
      181 cacaaaatcg tgtctgtgcg aatgcgggag gatacgctga aaaagctgga cgaactggcc
      241 gagcagacca accgctcccg aaacgagctg atcaacctgc tgctggcgga agcgcttcag
      301 gaggtatcag tggtggagga aaactgacgg ctttagccgt acccagcaac ggacataaaa
      361 atggggctgc gaaaaattct catagttgtt ccgcagcctc ttatttttcg tttttttcat
      421 cgttgcattt tggtgtttgg ccttctcagg atagcatttt ccccgttttc atgctatact
      481 ttccgtggtt aaaaatgctg atttcaaaag cccggagggg tttattttgc aaaagagcta
      541 tgcgttgttc gattttgacg gaaccctgcg aaagggggat tccatcgttt ccctgtgcct
      601 gtatgcccat cggcgaaagc tttgcaccac cggccagctg ctcaaaggta ttgcctacgc
      661 cgcaggctac gggctgcacc tgtgctcggc cgagcgcgcc aaggcggcgg cgctgtgctg
      721 gatgaagggg cggctcgtgc cggaagtgga ggccttctcc atggacttct gcctgcacgt
      781 gctggcgccc cagctgtacc cggaggggct gaaggccctt cggcaggagc aggcccgtgg
      841 agccagcgtc ctgctgatca cggcctcgcc ctcgttttat ctggagccgc tggaaaccat
      901 gctgggcgta gaggcggtca tcggcacccg aatgaacgtg gacgaccggg agcgctacac
      961 cggcgacatc tgcggcgata actgccgggg agtacaaaag cccctgcggc tggcggaata
     1021 tctggccgcc cggggcgacc ggctggatta cgacacctcc tcctcctacg gggacagcgc
     1081 cagcgacctg aacatgctgg agctgtgcgc ccggcaggtg ggcgtgaacc ccaagcccaa
     1141 gctgcgcaag gcgctggaag gccgcgaaaa cgccgagatc gtccactggg ctcccccgga
     1201 gaaagaataa cctatttccc tacaaaagca ctcaaaggag gaaccgccat gcagcgagcg
     1261 ccggactttt ctcaggccga tcaggtgatc caaaaagcgc tggaacagga ggtctttccc
     1321 gccgccagcg tgctggtggg ccgccgggag aaggtgctgt tccgccgggc ctacggcagg
     1381 ctttccattg aggaggacgc gggcctttgc aacgaacaga cccgcttcga tctggcctcc
     1441 gtttccaagc cgctggcggt ggggatgctg gctctgcggg ccatggaaag cggcaagctc
     1501 tgcctgtggg acaagctggg cgcctttatc gacgcgcccg ccgacaagca ggagatcacc
     1561 atcgcgcaga ttttgaccca tacggcgggc tttccgccgg ggctgcacct gtggcagctg
     1621 gcccgcacac cggagcagag caccgagctg attctggccg caccgctgga tttccagccg
     1681 ggaacccggg tgcagtacag ctgcacgggc tttatcctgc tggggcagct gctggaatgc
     1741 ctctacggtc tgccgctgaa tgagctggcc ctgcaggagg tcttctggcc gttgaaaatg
     1801 aaaaacacct cttacctgcc cgccgggggc aacatcgctt ccaccgaaat gcaggacgac
     1861 ggcttctgcc tgaccggggt cgtccacgat gaaaacgccc ggtttctggg cggagtcagt
     1921 ggcaacg
//
LOCUS       DAWAEA010000277         1923 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_7977_length_1923_cov_1.466274, whole genome shotgun sequence.
ACCESSION   DAWAEA010000277 DAWAEA010000000
VERSION     DAWAEA010000277.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7977_length_1923_cov_1.466274"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          442 a          580 c          558 g          343 t
ORIGIN      
        1 ggaactgcaa aacgcggaaa aacgcgtggt cggcaccaag caggtgcttc gcgccctcaa
       61 cggcggcatc gccgcccggg tctttctggc gaaggatgca gacggtttca tctaccaccg
      121 ggtcaacgcc ctgtgcgagg aaaccaaaac ccctgtcacg atggtggata ccatgcagga
      181 gctgggaaag ctctgcgccg tcggcgtcaa aaccgccgcg gcagccgtgc tcaagtaagc
      241 gcggcgtacc aaggaactat aaagtgtctt tgggttggac gctcttatag ttcaggaggt
      301 gcttttcttc aatgccgacg atcaatcagt tgatccgcaa gggacggcaa aaggctgcca
      361 accggtctac ggctcctatt ctgcagggct gcccgcagaa gcgcggcgtg tgcctcagcg
      421 taaagaccac gacccccaaa aagcctaact ccgcccttcg taagatcgcc cgtatccgtc
      481 tgaccaatca gatcgaaggt acgtgctaca ttccgggcat tggccataac ctgcaggagc
      541 acagcgtggt gctgatccgc ggcggtcgtg ttcgcgacct gcccggcgtt cgttaccaca
      601 tcattcgcgg cgctcttgac acggctggcg tggccaagcg gatgcaggcc cgttccctgt
      661 acggcgctaa gcggcccaag aactaagtaa ccctcgcgat tcagttactg aggcgatcag
      721 agatcgccca acgaaggagg aaatcaaatg ccccgtcgtg gatttgtccc caagcgcgag
      781 gttctgcccg atcccgtaca cgggaccacg gttgtgacca agctcatcaa ccagatcatg
      841 ctggacggca agcgcggcac tgcgcagcac atctgctaca ccgcctttga aaccgtcgcc
      901 gaaaagaccg gcaagagcgc ggacgaagtg ttcaacgccg ctctggccaa cgtcgctccc
      961 cagctggaag tcaaggcccg ccgcgtgggc ggtgccacct atcaggtgcc tatcgagatc
     1021 cgccccgaac gccgtcagac tctggcgctg cgctggctgg tggaattcag ccgcaagcgc
     1081 ggtgaaaaga ccatggcgga acgccttgcc gccgaaatca tggacgctgc caacaacacc
     1141 ggcgcggctg tgaagcgcaa ggaagaaatg caccgcatgg ccgaggccaa caaggctttc
     1201 gcccactatc gctggtaagc gatcccttcc cctggacgca ggcgcgtccc ccacacatct
     1261 taatgtgtat ctgaaaggag accctcatct atgcctagaa gtcacccttt ggaaaaggtg
     1321 cggaatatcg gcattatggc tcatatcgac gccggtaaaa ccaccacgac cgagcgcatc
     1381 ctgttctaca cgggacgtac gcaccgcttg ggtgagacgc atgaaggttc ggccaccatg
     1441 gactggatgg agcaggagca ggaacgcggt atcaccatca cttccgctgc taccacctgc
     1501 cagtggaaag gacaccggat caacatcatc gacacccccg gccacgtgga ctttactgtc
     1561 gaagtagagc gcagcctgcg cgttctggac ggagccgttt ccgtgttttg cgctaagggc
     1621 ggtgtagagc cccagagcga aaccgtttgg cgtcaggcca gcaagtacaa tgtgccccgc
     1681 atggcttacg tcaacaaaat ggacatcatg ggcgcggact tcttccgcgt tgtcagcatg
     1741 atgaaggaac ggctgggcgc caacgccgtg ccgatccagc tgcctattgg caaagaagcc
     1801 gatttcaagg gcatcatcga cctcgtccgc atgaaggcgg aagtctatta cgacgacgag
     1861 ggcaaggatg ttcgcgatga ggagatcccc gaggatctga aggaaatggc cgaggagtat
     1921 cat
//
LOCUS       DAWAEA010000278         1912 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_8018_length_1912_cov_1.841142, whole genome shotgun sequence.
ACCESSION   DAWAEA010000278 DAWAEA010000000
VERSION     DAWAEA010000278.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8018_length_1912_cov_1.841142"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    324..333
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          381 a          590 c          565 g          366 t
ORIGIN      
        1 agggcctgcg ctacgccatg gtcatcatgc tggaaaaaac gtcgcaggag aacgtgctca
       61 agctgcgctg ccggctgttc gagcggctgc agtatcagga cacccggtac ttcgaccgca
      121 accgcaccgg cgaactgatg acccggatgt ccggtgatct ggactggtgc cgtcacttca
      181 tgtcctacat gagctatgcc attgtggact gcgtgtgcat ctttttgagc accctgatct
      241 gctttttctt caccagctgg cggctgaccc tctttctggt gatggtgacg ccgctattgc
      301 tgctcatcac caatctgtac tccnnnnnnn nnngcccggc ccctgttcgt gttcatgcgg
      361 gaaaagctca gcgaaatgaa cgcgggggcg caggagaaca tcgcgggcaa ccgggtggtg
      421 aaggccttcg cccgggagag ctttgaagaa gaaaaattcg aaaagcgcag cgccgccttc
      481 cgggacagcc agctgaacat caataagaag tggctcagct tcttcccgtt tatcgaaatt
      541 ctggcccacg ccatgaccat catcacggta tttctgggcg gctacctgat catcaagggg
      601 cagctgactc ccggccagct gacgatcttc tcccagctga cgtgggcgct ggccaacccc
      661 atgcgccagc tgggcaatct catcaacgat ttccagcgct tccagacctc ggcccataag
      721 gtgatggagc tgtattgcag ccgccccgcc attcaggatc ggcccacggc gaaggatcat
      781 ccgtccatga agggcaaggt ggagttccag cacgtgaacg cggcctttga ccgcacggag
      841 gtgcttcacg acgtgtcctt cacggcggag gcgggcagca cgctggccat catgggcccc
      901 acaggctccg gcaagaccac gctgatcaat ctgctggccc gctgctacga cgtgaccggc
      961 ggcacggtgc tggtggacga taccgacgtg cgggactgga agctgcagca gctccggggc
     1021 gggctgggtg ttgccacgca ggatgtgttc ctgttctccg acaccgtgga cggcaatatc
     1081 gccttcggca atcaggagtt gacggaggac ggcgtgcggg aatgcgcccg catcgccgcc
     1141 gcggatgact tcatcgaggg cctgtcggaa aaatacgata ccatcatcgg cgagcggggc
     1201 accggcctgt ccggcggcca gaagcagcga atcgcgctgg cccgggcact ggccatgcac
     1261 ccgtccatcc tgatcatgga cgataccacc tccgcactgg acagcgagac cgagcattac
     1321 atccagcagc agcttctgca cctgcccttc tcctgcacca agatcatcat cggccagcgc
     1381 atcagcgccg tcaaggacgc cgaccagatt ctggtgctgg aaaacggccg catcaccgaa
     1441 cggggcaccc acgaggaact gctgaagaac cacggctact actggcaaac ctatgccctg
     1501 cagaacgacc tgccgatgga aggagctgct tcgtaatggc gcgcaacaaa tttgacgtgg
     1561 acgagacgct ggagtctccc tttcaatgga aacacctgaa acgggccttt cagtacattg
     1621 gccgccaccg cttccagatg ataggcgcgc tggcgctgag cgtgctggcc tccatctgca
     1681 ccctgtatac gcccaagatc atgtcccggg tgctggacga ggtggttccc gccggggatg
     1741 tgcagacgct ggtgcgctgg gcgctgatct actgcgggct gatcgtcgtc agcatcgtgt
     1801 ttaccgtcat ccgggcccgg gtgatggcct acgtcagtca ggagatcatc ttcgacatcc
     1861 gccgggatct gttccatcat ttgcaggagc tgcccttcag ctactacgac ag
//
LOCUS       DAWAEA010000279         1911 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_8030_length_1911_cov_1.391703, whole genome shotgun sequence.
ACCESSION   DAWAEA010000279 DAWAEA010000000
VERSION     DAWAEA010000279.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8030_length_1911_cov_1.391703"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1377..1474
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          389 a          509 c          518 g          397 t
ORIGIN      
        1 ttcggtattc agtatcccat tgagcgtcat gacccggacg gtgcgctgct tttgaccaat
       61 tttgccttgg aaatctgcgg cgcaaaggcc gactggaata tggacaccat cattgatctg
      121 gcgctggatg aaatcagaca ggacgcctct gccgacggca agattctctg cgctgtctcc
      181 ggcggggtcg acagcaccgt ctgtgcacgg ctggtcagtc tggctgccgg tgcccgcttg
      241 cgctgcattc tggtcgataa cggcatgttc catgaaggcg agccggaact gatccagcac
      301 gaatttctgg aaaatttggg aatctctctg gagcggatcg acgcacaggc ggaatttttg
      361 gaagccctcc ggggcgttac ggatgaaaaa cggaaagcga aaatcatttc cgaacgcatg
      421 attcaggttt tggcccgaca aatcaacgac gaccctgagg ttcgaaccgt cgttctgggc
      481 atgaatttca atgacgttct cttcgggcaa acgcccgtgg ctgcgctgca gaatgccaca
      541 actgaacgcc aaccgatcta tttgcagccc ctccaaaatt tgttcaagga cgaggtacgc
      601 cgcctggcgc agtctctttc gctgcccgct tctctttgcc tgcggcagcc ctttcccacc
      661 agcggactgg cctctcgcat tttcggcgaa gtgacggaga aacgtctgca gctggtacgg
      721 gcggcagacg cctgtctggc cgaagaaatc gcggaaagcg gctttgagcg caagctttgg
      781 caatattatt ccgtgctgtg tcaaagcccg gacatgcccg gctggtacac catcgttctg
      841 cgcgccctgc aggcgggccg taccacggct accagcgccc ggttgccttt tgacctgctc
      901 gaacgggtct cccaacggat gctgcgggag ctgcccggcg tttccagagt gggttatgat
      961 ctgactcctt cccgccatta cggcgagcag gaatagtctt tttcgtctca tcatacatca
     1021 aattttacga agggaagtgg tttggatgct gatctgcgac acccacgccg acacactcca
     1081 ccggatgcaa tataccaaga ataccgatat cacccttaaa aggctgaccc agccgggcca
     1141 tacgtgggtt caggcgctgg cgttgtttgt gggcggcaac ggactgaaag gcgacgatcg
     1201 ctatctaatc gagcgtgaat tggacaattt tgaaattttg aagaagaagg gtttccacca
     1261 gatccgcagt attgaggaag cgctgcccga caaggtaaac gccattctcg cccgcgagtc
     1321 cgcgctcgaa aagcatctcg cggaggaggg aagaaaggaa gaatccgctt ctgcaannnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnctgatt tggaacaacg ataatctgct
     1501 ggcccacccg gccgtcaagg ggtctgacaa cggcctgact cctttcggct ggcaggtgac
     1561 caaagaactg cgcaagtacc atattgcgct ggatatttct cacctgaatg agcgcggttc
     1621 ctgggagctg atggaaagca acgttccgcc tatggcctct cacagctgcg cccgggcgtt
     1681 gtgcgaccat ccccgcaacc tgacggacga gcagcttcgg gcgctgttcc acgcaggcgg
     1741 ctatgtgggc gtaaacttct acccggtctt tttgaaggag tcgggtcagg cgacggtaga
     1801 tgacctgatc gaccatatcg cctatatgtg tgatcttggc ggcgagcgct gcgtgggcct
     1861 tggcagcgat ttcgacggaa tcgactgcta tcccgaagcg ctacgaacgc c
//
LOCUS       DAWAEA010000280         1909 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_8037_length_1909_cov_2.215210, whole genome shotgun sequence.
ACCESSION   DAWAEA010000280 DAWAEA010000000
VERSION     DAWAEA010000280.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8037_length_1909_cov_2.215210"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    658..753
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          299 a          616 c          505 g          393 t
ORIGIN      
        1 gtccctgcga cgattgagtg gcagcagtgc ctcctgcacc tccacttttt tgacacactg
       61 aaagaagcgt ccgaagacgc ttcttttgga aggttgattg agttgtcgaa agaatgccac
      121 cttcgggaat tactcctcat ccagcagctc cagatcggga tcttcgtcca cgtcggcgtt
      181 ggcacgttct tcgtcggtgg gggcttcttc cttggctacg ccctttccag cggccaaagc
      241 ctgcttggct tcgtcccgga tgatcttgtc gatctcggcg gcgatttcag ggttgtcctt
      301 caggaacagg cgggcgtttt cacggccctg tccgatgcgc tgatccttgt agctgaacca
      361 cgcaccgctc ttgtggatga tgtcccgatc cacggccata tccagcagcg taccctcagt
      421 ggagatgccc tcgccgtaga tgatgtcaaa ctcggccacc ttgaagggcg gggccacctt
      481 gttcttgacg accttgacct tggtgcggtt gccgatcact tcggagccgt tcttcagctg
      541 ctcaccccgg cgcacgtcca gacggacgct ggcgtagaac ttcaacgccc ggccgccggt
      601 ggtggtctcg gggttgccgt acatcacgcc caccttttcg cgcagctggt tgatgaannn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncttgcgc agggcctgac tcatcagacg
      781 ggcctgcagg cccacgaagc tgtcgcccat ttcgccctcg atctcggccc ggggcaccag
      841 cgcggccacg gagtcgatga ccaccatgtc gatggccccg gaacgtacca gcgcttcgca
      901 gatctccagc gcctgctcgc cggtgtccgg ctgggaaatg tacagctcgt ccacatttac
      961 gcctagcttc cgggcgtaga cgggatccag cgcgtgctcc gcgtcgatga aggcgcatac
     1021 gccgcccatc ttctgggcct cggccacgca gtgcagagcc acggtggttt taccgctgct
     1081 ttcaggcccg taaatttcta ccacgcggcc ccggggcaga ccgcccacac ccagcgccat
     1141 atccagctcc agacagccgg tagggatcac ctgcagattc gcgtccgccg ccgcgtcgcc
     1201 gaatttcatg accgcgccct tgccgtaggt cttgttcagc tgggccagag cgctttccaa
     1261 cgctttcatt ttttcttccc gaagatcgcc ctgcttgctg gcgatctctt ttttggccgt
     1321 atccttcttc ggagccatgc ccttttcccc cttacgtatc taagtcgaat gatcgtttgt
     1381 tatttagaat gcaggacgtc cagcgccagc cgcagcgccg tttctaccgt ctggcggcgg
     1441 acgctctgcc ggtcgccgtc aaagtgaaag cggcgggcga cagttccctc ggcagtgctc
     1501 atgcccagcc agaccgtgcc tacggggttt tccggcgtgc cgccggtagg gccggccacg
     1561 cctgtagcgc tcagggccac gtccaccttg agcagattcc gcgccccgga ggccatctgc
     1621 agggcacagg cctcgctgac cacgcccacg gtgtcgatca cttcctgcga aacgcccagc
     1681 acctcgtgct tcacacgggg gtcatagctg accacgccgc ccatcagcac cgccgacgcg
     1741 ccggacacat ccgccaccgt agcggcgatc aggcccgccg tcaggctttc cgccgtccca
     1801 agcgtcagcc ccttttcggt aaattgccga accacctgcg gagccagaga ggactcgctc
     1861 atttggtttc ctccttcagg ctcagtacgg aacggttctt ccagaaata
//
LOCUS       DAWAEA010000281         1901 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_8064_length_1901_cov_1.711809, whole genome shotgun sequence.
ACCESSION   DAWAEA010000281 DAWAEA010000000
VERSION     DAWAEA010000281.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8064_length_1901_cov_1.711809"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    243..267
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1028..1091
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          358 a          540 c          488 g          426 t
ORIGIN      
        1 ttcgtgccag atggaaagca cgctgcccag tgcgatattg gttttgccgc cggtttgagc
       61 atagatttcc ttaccgaaag taagcaggtc gtacaggggc tgttccaccg ccaccggcgc
      121 gatcccagcg ttttggttca cggtataaag attattcacg ccctcgtaag cctgataaat
      181 atcgaacagc cggtggtaaa ccttcagttc gtcatggacg gtctgcaaaa tttcctgcgc
      241 agnnnnnnnn nnnnnnnnnn nnnnnnntgc gtgtccgcat agaccaccac ctgtcaaaac
      301 gtgtcgaaca cgtccagaaa gctggtctcg taccgtttgc tttccgcggc cgcacagggg
      361 acaagggccg cccaaagcag cagcgccgcc atccatcccg ccagtttcct catgtttttt
      421 cctcccaacc tgtttccgtt tcgctatgaa aagtttcttc cccgggctgc cggattcctg
      481 ttttctgctg taaaacgcct ttttctccat actaccgtca aacctatgca ttttcccgat
      541 tctgtgctat aatgaagaac tgtgcaggat tctgcacctt agttcctgag gcagggggag
      601 gaaacgtcgt gacgcttaaa cccaaaacgc agaaagccgt ccgcacgggg ctgtggcttc
      661 tgctggacgc ggttttgatc aatctggcca tgatcctttc gcaggttctg cgctatacca
      721 ccaccatttc ttattctttc tttcagattt atttccgtat cgccccggct atgactttga
      781 tggggctgat tttgtttgcc gttttcggcc tttaccgcac catgtggcag tacgcttcgg
      841 cggaagacct gctgcgggtg gtactgtcca cgctggcgct ggcggtttgt acctatctgt
      901 attccgtcat cgccaattat tttgtgcggc cccagaatct tttccggctg caccggctga
      961 tctacccgct tttctggctc atcagcatgg cgctggtggg cgcatcccga ctgatctacc
     1021 gctatgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nttggctggg ggccaatgtg gtgcatgaga tgcagcacgg aaactatggc
     1141 agctgtgtgc ccgtcatcgt ggtggatgac gaccagcagc gcaccggctc cagcctgagc
     1201 ggcgtgcccg tggtgcgcgg cagcggcaat attctcaagc tggccagcga ttattccgtc
     1261 gacgacatca tcatcgccat ttccaccccc aaggccgacc tgaacccggt gatcgaaaaa
     1321 tgccttgcta ccggctgccg ggtcaagcgg gtcacgcccc tgcagaatct ggaagggagc
     1381 cggcccagcg ccggggccct gcgggaattg aacatcggcg atctgctggg ccgcccggag
     1441 gaacagctgg atatgacgca ggtggctgcc tttgtgcggg ataagaccat cctcatcacc
     1501 ggcggcggcg gaagcatcgg cagtgaactg tgccgccagc tctttcccct gcagcccaaa
     1561 cggatcgtcc tatatgacat cagcgaaaac tatatgtacg acctgtattc cgatctggaa
     1621 cgggaatacc ggcaggatct ttctcaaaag ctgatcctgt gcgtgggttc catccgggac
     1681 gaacagcggc tggatgaagt tttcggctgc tacaagccgg agatcgtcct ccacgctgcg
     1741 gctcataagc acgtgcccct gatggaggac tgccccgatc aggcagtgaa aaacaacgta
     1801 ttcggcacca acctgacggc gctggccgcc atccgccacg gggtcaggcg cttcgtgctc
     1861 atttccaccg acaaggccgt caaccccacc aacgtcatgg g
//
LOCUS       DAWAEA010000282         1896 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_8086_length_1896_cov_1.777295, whole genome shotgun sequence.
ACCESSION   DAWAEA010000282 DAWAEA010000000
VERSION     DAWAEA010000282.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8086_length_1896_cov_1.777295"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          339 a          645 c          521 g          391 t
ORIGIN      
        1 gcctcatgct ccgcgctgat ctcccggtac agacggcagg aatcccactg accgttggtc
       61 tcggtcagca tggctttcag cggcaccggc gggattttca tctgccggaa cgtctgcaga
      121 agctggttca tcagcatcgg cgtaaagccc gccatcacca gcatttcgtc aggaaaggtt
      181 tcttcgcagg gaacattttc cccgcctgtt tccagtccgc acaggtcgtt cagcgtcagg
      241 ccgtattcat cgggccgaac gtcccgcagc aagacccgca gccgcatggc ggcaaagcga
      301 attttcgacg ctttttctcc cgacagatta tagcaaagca gcgtaggctt catcgttctt
      361 tcctcccaag ccgaaaatga ggtttctccc ctcatggaga taccccatct tagcacgttt
      421 gaaaccacaa atcaaagccc ggacaggttc gaccggcccg ggctgaaaaa cttgcttatt
      481 tttcggtttc cccaaagaac ttttcttcca gcatggcgca cagggtatgg taaatgggca
      541 gatggtattc ctgcacgtcg gcgggcgtat gggccggagc gatgatagcg cagtcgcaca
      601 gttccttcag ttgtccgccg ctgccgccgg tcaggccgat agtcgaaagg cccagcgctt
      661 tggccacccc aacggccagc gccacgtttt tggcgttgcc gctggtgctg atgcccagaa
      721 ccacgtcgcc gggctttccg tatcctgcca cctgctgagc cgccgtcagc aggggttcca
      781 gatcgttctg cacagcggtg gacagcgctc cgtgctgggt caggtcgatg gcgggcagcg
      841 cctgctgcag cagttcagcc gtccccggca gcaggctttc cgtttgagcg gcgatcacgg
      901 cgcgcttttc ttccgggaaa ggccgcttca ggtaaaagcc cttcatcagc tcgcctacga
      961 tatgccccgc gtcggcgcag ctgccgccgt tgcccacgat cagcagcttt ccgccccggc
     1021 tgtagcaccc gcgcagcagc agataagcct ccatcaccga gcctcgcagt tcatccagcg
     1081 ccgggtagcg gtggaacagc cgctcgtact gttccaacac gcgggatttc cgctcgctgg
     1141 tctcttccat ggggtccacg tcacagccgt acagtgccgc cataatactg gccagatcgc
     1201 ccagtgcctc tcccgtctgg gccggaacga cctgcacccc ttccagcgaa aaaggaatcg
     1261 cctcccgctt caattccgct tccatcgccg gacgcagcag gtcttcgcag cgcacaaaaa
     1321 tgctgccgat gacgatcttt tccgggttga gcgtgtccgt caaaagggcc agtcccttgc
     1381 ccagcatggt tccgatatgg tcaaagaagg ctttcgcgtc cgcgtctccg gtccgggcat
     1441 aggccgccag cagcttcgcg tccgcttctt ccgggccgtg tccgtccctg acccatgcgg
     1501 gagtgtgtcc ctcccacacc attttttccg tcagggcgcg agcctgccgc tgaatacctc
     1561 cgccgctggt gaatccctca aaggagcccg ccttgccaaa gcccaccggg ccgtcctttt
     1621 ccagccgcag atggccaatc tcgccgccca tatccgtgca gccccgaagg agctttccct
     1681 cggcgatgat gccgcctccc atgccggtgc ccatggtcag gaagatcata tcgctgcatc
     1741 cccggcccgc gcccaatttc cattccacca gtgcgcaggc gttcgcatcg ttctgcaggt
     1801 agaccggtac gccgcagcgt tcctccagca tggctttcag gggaatgttg acccagccgg
     1861 gcagattggg cggacacagc accatgcccc ggcggg
//
LOCUS       DAWAEA010000283         1884 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_8137_length_1884_cov_2.402406, whole genome shotgun sequence.
ACCESSION   DAWAEA010000283 DAWAEA010000000
VERSION     DAWAEA010000283.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8137_length_1884_cov_2.402406"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          387 a          536 c          550 g          411 t
ORIGIN      
        1 gcggggaatg gcgaaaaacg gcccaatggt cagatacagc gcgcaggtga agaagtagct
       61 gtacgggcgg ctgaccttgg agctgagctc catcaggccg tcgctgcgac tgatgcccat
      121 ggcggcgatg cccagaaggg gcaggcctac gccggtaatg acgaagccaa tgatcgcctg
      181 ccacatattt tttccggcga gctgtcccat gtacgcgggg aagatcagat ttcccgcgcc
      241 aaaaaacagg ccgaagagca tggtagcgat gagcacgttt tccttttttg tcaaccgttc
      301 cttcatcccg atcacatcct tgtgcattat atttgcaata atataacacc aattctggta
      361 tggcgcaagg acaatttcaa taaagtacgg ctccggaaat tgtatttata ttgttttttc
      421 ttttcggacg aaaacagaac cgtttccgcc cgccttttct gccctgcagg cggtttttct
      481 tgcgctgggg cccgttttcc cgtataatgg cgttgccgtc ttcttccctg ttttcttttt
      541 gattttattt tggactgata cggaggactg caacattatg caacagaaag gaatggtttg
      601 ggtcattgga gcatcgggcg gactgggccg cgctacggcg caggccttcg cccaaaacgg
      661 ctggagcgtg attgcggggg cccgctcctt cgctggggga gggcgttacc gtccttccat
      721 tggacgtgac gagcgaggaa agctgcacgg cctttgtgaa aaaggccctc ggcatggaaa
      781 gccgggtgga tgtgctggtc tgcgctgcgg cgcagctggt gctgggctcc tgcgaaaata
      841 ccacggtgga cgaattggac aggctgctgg acgtcaacgt gctgggcgcg gtacggatga
      901 tccagcgggc cctgcccctg atgcgcgcgc aaaaaagcgg aaagattatt ctgttctcat
      961 ccattaacgg gcttctgggc attcccttcc agagcgccta taccgccagc aagcacgcgc
     1021 tggaagggta cgccgagtgt ctggccatgg aaaccgcaga tttcggcatt caggtctgtc
     1081 tggtagagcc gggcgaccat cggggcggca gcagccacac ccgtcttcac gccaaaaatg
     1141 aaacgaacac cgtttaccag aaggcgtatg aaaacacctg cgccatcatt catcacgacg
     1201 aagaaaacgg cctctttccg gaccggctgg gccggaaaat cgtgcggaac gcccagcgac
     1261 ggcatatgcg cttccgtctg agggtcgcca agcccgacca gcatctggcc gtgcttttgc
     1321 atacccttct tcccgccagc ctgaatcgga aaattctctg ttcctattat cgtgctcatc
     1381 tctgagacaa gtctctgaag gtacattctg aggaggaaaa aaattgaatt catcttacgc
     1441 tttggagcac aactgtgctc ttcttgagca atccaaaacg ctggagaccc tgttccgcct
     1501 gacctgcggc catggggacg cggacgctgc gctttttctg gaaaacggcg aggaaaagcg
     1561 ctggtcgtac cgccaatatg aggccatgac ccggcaatac gccgcctgcc tgcgccgggc
     1621 ctttccggag gagggttata tcgccatctc cgtggacacg tgcaaggagt ggttctccct
     1681 gttctgggga gtgatcctct ccggccacaa cgccctgctg atcgacgcgg cctgcgccga
     1741 cgatacggct ctgcggcttc tgcgggaggc cgactgccac gccattgtca cccagcgccg
     1801 ccgcaccctg ccgcaggacg tgcgcagtct ggacttctcc gcgctggtgg ggcaggcggc
     1861 cgccgagccg gaggacggct tccg
//
LOCUS       DAWAEA010000284         1878 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_8175_length_1878_cov_2.136039, whole genome shotgun sequence.
ACCESSION   DAWAEA010000284 DAWAEA010000000
VERSION     DAWAEA010000284.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8175_length_1878_cov_2.136039"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          351 a          544 c          638 g          345 t
ORIGIN      
        1 tccgggaacg gggcctgcat ctggtgctgt ggcagatccc cgccctgaag gcgctgaagg
       61 actgcccgga cgcggagccc tcccgccggg actgggagga ggccgtgtcc aaagggtatg
      121 tgattcgtca ggaggacggc gcgccctatg tgattccgga caagtggttc gccggaagcc
      181 ttctgccgga tttcaccaac ccggaagcct gccgctggtg gtttgacaag cgtcagcctc
      241 tgctggaaat gggcgtggaa ggattcaaga ccgacggcgg cgaattcctg tttggcagca
      301 atatccgtct ggcggacggc acgccgggcg gcgaggccca caaccgctat cccatgcagt
      361 atgtgaaggc ctatcaccgc tggatgaaag aaaacggcgt ggagggcgtg acgttcagcc
      421 gggccggata cacgggcgcc cagaccgtgc ccattcactg ggccggagat cagctgagca
      481 cgtgggaaga actgcggggt cagctcagcg cgggtctcag cgccgggttg tccggcgtgc
      541 tgttctggag ctttgatatc ggcggcttcg ccgggaaaat cccggatcgg gagctgtacc
      601 tgcgggccac ggccatgggc tgcttctgcc cggtgatgca gtggcacgcg gagccgcgaa
      661 acggccagtt cttcgccacc ggcgatccgg aatggaacaa cgaccgctcc ccgtggaatc
      721 tggccagccg ctggaacgcc ccctccattg cggaaatcgc ctgcgccttc gcccggctgc
      781 gggagcgtct gcggcccatg ctctacgaag aagcgcagtt ctgcgtgcag gagggtcgtc
      841 ccatgatggc ccatctctgt ctggactttc cggacgatga acaggcgctg gcctgcgggg
      901 acgaatacct gctgggccgc cgtctgctgg tcgcgcccat cgtccagccg ggggcggagg
      961 gacggcgggt ctatctgccc aagggccgct ggaagcattt aacggcaagg tctacgaggg
     1021 cgggcgaacg gtggaactgt cctgtcccct gaacgaagcg ctggtttttg agcgggagga
     1081 ggaagaaaca tggaactgcg caatatgacg atctattcca tctttgtgcg caatcacggc
     1141 ggaacctttg ccgccgtgga aaaggatatt ccgcgcattc gggcgctggg ggcggacgcg
     1201 gtgtggctgc tgcccattca cccgatcggc cagaaggaac gcaagggcgc actggggtcg
     1261 ccctatgcca ttgcggatta ccgggccgtc aacccggaat acggcacgat ggaggacttt
     1321 cgccatctgg cggacgccgt tcaccaaaac ggcatgaagc tgatgatcga cgtggtgtat
     1381 aaccacacct ccccggactc gtggctggcg cagcatcacc cggaatggtt ctaccataag
     1441 ccggacggca gcttcggcaa ccggatcggc gaatggtcgg atatcattga tctggattac
     1501 ggcaatcccg gcctgtggga ctaccagatc gagacgctga aaagctgggc ggagatcgtg
     1561 gacggcttcc gctgcgacgt ggccccgctg gtgccgcttg atttttggct gcgggcccgc
     1621 cgggaggtgg agacggtgcg gccggactgc ctttggctgg cggaatccgt ggagccggag
     1681 ttcatccggg acaaccgaaa ccgcggcatg gtcagccaca gcgactgcga gctgtatcag
     1741 gccttcgacc tttgctacga ctatgatatt tacggcgatt ttctgtccta tgtgcagggc
     1801 aaagccgaac tgacggacta cgtaagggct ttggagcggc aggagagtat ttatcctgcg
     1861 gactacgcca agctgcgt
//
LOCUS       DAWAEA010000285         1869 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_8222_length_1869_cov_1.777288, whole genome shotgun sequence.
ACCESSION   DAWAEA010000285 DAWAEA010000000
VERSION     DAWAEA010000285.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8222_length_1869_cov_1.777288"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          309 a          588 c          603 g          369 t
ORIGIN      
        1 gccgcgcccc tgcgtctgtt tctcttcttt ttcaaacacc tgcatcggcc actcgtgttc
       61 cacggacggg atgcggatgt gcgtcagctt ttcaatatcc gccagatatt ccttttcctc
      121 aaagtcgcag aagcttacgg ccacgcccgc gtgccccgcg cgccccgtgc gcccgatcct
      181 gtgcacatat gtctcgggga tattgggcag atcatagttg aacacatggg aaaggccgct
      241 gacgtcaatg ccgcgggcgg caatgtccgt ggccaccagc acgcggatac ggccgtcctt
      301 gaagcggccc agcgcgtcct gccgtgcgtt ctggcttttg ttgccgtgga tggccatagc
      361 cccgatgccc gcgcgggcaa gctcccgcac aacgcggtcc gccccatgct tggtgcgggt
      421 gaacaccagc gcgctggtca cctccggttt ttgcagcagg ccgtcggcca gctcctcgat
      481 ctcccgcggc atggtggccg agaacagcag cgtctgcttt tgccccggaa ggcaggcgag
      541 cacccgcttc acatcatgta caaagcccat gtccagcata cggtcggctt cgtccaatac
      601 gaatatctca acatttttca ggtcgatata gccctgtccg atgagatcgt tcaggcggcc
      661 gggcgtggct gtcaggatat ctacgccctt tttcagccgg gccacctggg gattctggtt
      721 cacgccgccg aaaataacgc agtgtgcaag gcgcatatgg cggccgtagg ccgcaaagct
      781 ttcgtcgatc tgcagcgcaa gctcgcgggt cggcgtgaga atgagcgctc ggatggggcg
      841 cccgcccgaa ggtgctggcg cagccgccag acgctgcaga atgggcatgg cgaacgcggc
      901 ggtcttgccg gttccggtct gggcgcagcc cagcaggtcc cggccctcca gcaccggcgg
      961 aatggccttg gcctgaatgg gcgaaggctg ggtgtagccc tgctcttcca cggcgcgcag
     1021 aagggggcgg gtaagggaaa gttcactgaa attcatagat gatgctgctc attcctgcgg
     1081 ggaatgcccc gctgtgtagt gggtattcct ccgccgggct gggcggagca tatacggttt
     1141 tattatagca cgatttacag caggatgcaa aaccgccttt gggcctttcg aggggctgct
     1201 tttccgcaaa acaaagcccc ctcggtgagg gggccggacc gccggctttg cctgcgggca
     1261 gcttctcaca agggggcttt gcagtgtgac ggatcgtgtt tttggggtca gccctgaatc
     1321 tcccgggctt ccagataata gcgcttttcc tcggcatggc ccatgctgaa ggtcttgcgg
     1381 ggcagcacgc cgcccagcac aacgcccttc aggatgtcgg ccttgtcaaa cgggggcagg
     1441 atgaggccca cggccccctc ggccaccatg cggcgggcgg tgtcgtcgcc gtggatgtag
     1501 tccacccggc tgccggggca tttggccagc acctgctgca caaagttttc ggcggtgccc
     1561 acggccagcg gctcggcggg ggtgtcaaag ctgacggcgg tttcccggcc gtccggcgtg
     1621 gccagggtga agctctggcg gcccttcggc gcctcgccca ggtgcaggcc cagatggcgc
     1681 tcctcgccgt aggcgtgcag ctggcgcagc acctcgtcgg cgctcacatc aaacagcacc
     1741 cggggaatgg actcgatctg gatggaatcg ctgtacaggt tgcacacctc ggccaggcac
     1801 cagcgggcgg gatgagtctc ccgctgctcg gggggcaggg tggacttcag ctcctcccaa
     1861 taggccttg
//
LOCUS       DAWAEA010000286         1849 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_8313_length_1849_cov_1.597547, whole genome shotgun sequence.
ACCESSION   DAWAEA010000286 DAWAEA010000000
VERSION     DAWAEA010000286.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8313_length_1849_cov_1.597547"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          347 a          549 c          573 g          380 t
ORIGIN      
        1 tgctgcacga tgccgatggc cctgcgcagg gattccagcg ttacggtttt cacatcgcgg
       61 ccgtcgatgc acacatggcc ggacgttgcg tcgtaaaaac ggggaatgag gttgcacagc
      121 gtcgtcttgc cgccgccgga cggccctacg agagccagct tttcaccggg gcgcacatcc
      181 aggcttaaat cgtgcagtac tttattgtgg tcgtccggat attcaaagct cacatgctcg
      241 aaggtgatgc gtccctgcac attttccagg ggctttgcgt tctcgtcgtc gaagatgtct
      301 acatccgcgt ccatgatctc gcagaagcgc tcgatgcccg tcatgccccg ctggaactgc
      361 tccgcgtagt ccaccacccg gcggatggag gtcagcagcg tggaaatata cagaagatac
      421 gccatgtagt ccgccgcggt gatcttgccg tcgatgatga acagcgcgcc cacgatgacc
      481 accacgatgt acatcacgcc gtcaaaggag cgggtggtgg tgttgtagcc cgccatatag
      541 cggtatttgc ggcggccgat gcggacgaac tcctcgttgc cccggtcgaa tttatcgatt
      601 tccataggct cgttggcgaa gctcttcacc acccggatgc ccagcaggct gtcctccacc
      661 tgagcgttga tctcgcccac ctgatggcgg ctctccttga aggctgcgcg catcttcttg
      721 ttgaaatgcc gggccagcac cagcatgatg ggcagcatga ggaagatgat cagcgtcagc
      781 accggattga tgcccagcag gatcacaaag ggcacggtga tcttgatggc ggcgatcagg
      841 aattcctccg ggcagtggtg cgcgaactcc gttacgtcga acaggtcgct ggtgatgcgg
      901 gccatgatct gccccacctt ggcgttgctg taataggcga agcccatctc ctgcagatgc
      961 tcaaacaggg cgtggcgcat gtcccgctcg atataggtgc ccatcacgtg gccccgggcc
     1021 accatgatgt agttggccac agtatcgacc aggcgcagaa gcacgtatac gccgcccacc
     1081 ttcagcacat aggccacggt cagggcggcg gcgccgctgc tggccagtcc ggtaatgctg
     1141 cgcacgatca tgggcagcac caggtcgcac aacgtggtca gcatggcgca gaacaggtcc
     1201 agcgccagca ttccccgata ggggcggaaa tagggcagaa agcgccggac cagctcggag
     1261 gttttcatgg tgcgttggtt gccgttggtg gatttcgttt gcatgggaat gatgctcctg
     1321 ttctgaatgc tcctttggta tcggttgaaa aaattattct gcggacggcg cggcaatcag
     1381 ggtcgtctgc ttcagcagta cttccagatc ggccttgtgc ccgatggggc agcggacggc
     1441 gtaggccgcg tccgccgtct ggccgaagac cagcacgtcc gcgccgtttt ccatccaggc
     1501 cgcgtccatg ccgccgatgt agtacatcgt gtcgccgttg agatgataat cctcgccctt
     1561 cagcagggca atggccgaaa cgggacggat gctttccacc gtcagggatt gtccgtcctc
     1621 aaaggcatag gtcagcgttg cccggcgggc gtattcgccc tgaaaagcgg tgtcgtacac
     1681 ccgggaacgg gtcatggaca gggcgctgcc gtacaggcac aggaccggct ggccgaagat
     1741 ctccgccagt ccctccgggc tggtatcgcc ggggctttcc acggcgctca tgggcagggt
     1801 gatgggctgc gtctgcgaag gctgctgggt cagctgctcg tcctcatcc
//
LOCUS       DAWAEA010000287         1839 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_8363_length_1839_cov_2.714686, whole genome shotgun sequence.
ACCESSION   DAWAEA010000287 DAWAEA010000000
VERSION     DAWAEA010000287.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8363_length_1839_cov_2.714686"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          314 a          562 c          559 g          404 t
ORIGIN      
        1 ttatttcgat tttcagctta gcccttgccc tgaccgatca ggttgatcag gccgccggcc
       61 atcagcatat cccgctggcg ttcggtaacg gacaggttca tcgaaatggt gaagcccttg
      121 gtcacgttct gcaccgtcac cttgccggtg gcctcggcca cctcccggcg cacgtcagga
      181 agggcgatct catcgtcccg gtcgaaacga tcgtagtcgc tttcattcgc aaattccagc
      241 ggcagaatgc cgttattgat caggttggcc cgatggatac gggcgaagct cttggccagc
      301 acgccgcgca cgtgcaggta caggggcacc agcgccgcgt gttcccggct ggagccctga
      361 ccgtagttat gaccggccag caggaatccg ccgcccgccg ccttggcgcg ggccgggaag
      421 gtcgcgtcca ccggggtcag gcaatgttcg ctgagcttgg gaatattgct gcggaagggc
      481 agcaggtcgc tgccgctggg catgatatgg tcggtggtga tgttatcctc cattttcagc
      541 aacacctgcc cggaaaggct gtccgccagc gcgtctgcct tggggaaggg ctggatattg
      601 gggccgcgga cgacctccac ggaagcggct tcttccggcg aagccggggg aagaatcata
      661 ttgtcatgca ccgcaaaatg ctccggcatt tccaccgtca ggtcggcatc cgtctcacgg
      721 ggatcggtta gcacgccggt cagggcggtg acggcggcgg tctcgcagct ggtgaggtaa
      781 atgcctgcac tcttcgtccc gctgcggccg aagaagttac ggttattggt gcgcacgctg
      841 acgccgttgg tcttgggact ctgacccatg ccgatgcaga agccgcaggc ggtctcgcag
      901 atgcgcgcgc ctgcttcgat catatcgtgc agcgcgccgt tctggctgag catactcagc
      961 acctgacggc tgccgggagc gatgaccagc gacacctcgg gattcacggt tttgcccttg
     1021 aggatggccg ccacgcgcat catatcctga tagctggaat tggtgcagga gccgatacag
     1081 acctgatcca ccttgatggg gcccgcctcc cggacggttt tcacattgtc gggcatatgg
     1141 ggcaaagcga ccatgggttc cagcttggac aggtcgatgt ccacttcttc atcatattcc
     1201 gcgtcagggt cggcaaacag gggctgatag tcttcctcgc gctcctgcgc cttcaggaag
     1261 cggcgggccg cttcgtcgct ggggaacacg gaggtcgttg cacccagttc cgcgcccata
     1321 ttggcaatgg tggctcgttc ggggatcgtc aggctttcca cgccctcgcc ggtgtattcc
     1381 atcaccttgg ggcagttcag gtaatatgcg ccgcctccca tagccacggc cacgtccagt
     1441 ccgcccgcgc caatggccag cattcccagc gcgccgcctg tgggggtatg gctatcgctg
     1501 cccagcagcg tccggcccgg cttgcccacc cgttccagct gcacctgatg acagataccg
     1561 ttgccgggct tactcaaata gataccgtgc tttttcgcca cggtggtaat atactgatgg
     1621 tcgtcggcgt tttcgaagcc cgtctgcagg gtattatggt cgatatacgc catgctgcgt
     1681 tccgttttca cccgatccac gcccatagcc tccagctgca ggtaggccat ggttcccgta
     1741 gagtcctgcg tcagggtctg atcgattcga atagcgattt cctcgcccgg caccagctcg
     1801 cctttgagca ggtgattttt tagaattttc tgtgtcaga
//
LOCUS       DAWAEA010000288         1837 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_8376_length_1837_cov_1.560045, whole genome shotgun sequence.
ACCESSION   DAWAEA010000288 DAWAEA010000000
VERSION     DAWAEA010000288.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8376_length_1837_cov_1.560045"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    332..381
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          345 a          583 c          482 g          377 t
ORIGIN      
        1 acaacctgcg ccggaagttt cagcccatcg cccgggaaat gggcttcgtg tgcgaaaacg
       61 gcggtctgaa cgcggtgggc cgagagatcg tcagcatcca ccccatggat cgcgccgctg
      121 cgctgggcat tacccagacc ctgcaagggc ttggattgag tattctttta agcggcgttc
      181 gctgctgcta tacgctggcc agcgaccgtc ccttctgcga cagcatggtc tataccctgc
      241 gcaacaccat gaccatcgtg gacgattttt cccagatcga cgatgatttc atcaagctgg
      301 cagcctatca tcccggcacc atcgacccct tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn ncgcaaatgc gaccacgtat tggacgtatg gcgcgccatc
      421 attcgccacg gcggcgaact gccggacgtt atcgaataaa attggttctc ataaacggtt
      481 ctcgctccgg atggggcagg ccgtttttct ttcccagttc gatcagcttc agcatccacg
      541 ccatattctg ggccagcgtg cgcatgatct gcaggccttc ttcgtccttc cgcacgtcct
      601 cgggctttgc gccgtgcacc tgattccagt actgggaggg caccacaggc atacaggaaa
      661 tggtgaagaa gcggttgata tcgtcaaagg tggacacgca gcctccccgg cgggcgctga
      721 ccaccgctgc agcgggctta tactgcatat ggttcttata ggcgtagaac agtcggtcca
      781 taaaggattt cagctggccg gaaatgcccg cgtagtacac cggcgtaccg aacacatagc
      841 cgtcgaactc atccagcctt gcgccgatac ggtccaccgc gtcgtcgaac acgcagcgtc
      901 cctcccggcc gcaatagccg cagccaatac agccgtggat gtcctcgtga cccacgtgga
      961 gaatctccga atccacgccg cactttttca attcccgctg cacttcctcc agcgcggtat
     1021 acgtacagcc cttttcgtgg gggcttccgt tcaaaagcat cactttcatt gtttctttcc
     1081 tccgtttcaa cgctgccgca tgggctgccg cccctgcacc ctgccaaggg ctatgccctt
     1141 gacccatttt tgaccgtgca tagcacggtc aatgtcggga ttgctaaggg cgaagccctt
     1201 aactgcgggg tttggggccg cacaggcccc atcgtctccc cttattagta tgccacgttt
     1261 gagagggcca tgcaagggtc agcgggcggg gaaccacatt tcggaggaaa cggcgaaggg
     1321 gctgtccttc tgaacggcgc tcaaaatacg cgccgcgtcc tcgccctgcg cgaataccgg
     1381 actgaagtcg ttttccggcg cagagccgga ggtcagcacc ggcagcagtc gcagcgacac
     1441 gcccgcgtag ccctgctcgt caaaccgcag cgccgcctga accaacaggc cgtcgaaggt
     1501 tttcattttg tgggtgccgc caaacaccag attgcccaga ctatagacaa tcaccgcgcc
     1561 gtcctgccac atgagggtga gtcccgatca ccagatcggc cccgccgttg atcgcctccc
     1621 gggccatttt ttcctgccgc tcgttatgcc gggcggcata ttccttgccc cagtggcagg
     1681 agtagacgat cacctcgcag ccttcctccc gcagggcctg caggtcctcc cgcatgatct
     1741 ccggcttttg ccgccagacc gtctcccggc agccgccgaa accaatcttg tggccgcgaa
     1801 tctccgtcac ataacgatag gtgtagccgc tgtaggc
//
LOCUS       DAWAEA010000289         1830 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_8412_length_1830_cov_2.080563, whole genome shotgun sequence.
ACCESSION   DAWAEA010000289 DAWAEA010000000
VERSION     DAWAEA010000289.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8412_length_1830_cov_2.080563"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          354 a          600 c          544 g          332 t
ORIGIN      
        1 catacggtct atccgccgct gccctcgctg attcctctga cctgcggcgc agcatgggtt
       61 tccgccctgc agggcttgag cctgaaaaac gtacgactga ccctgaaacg gggcaagaaa
      121 gtcctttata gcgacgtggg cgagatgctt ttcacccact tcggtatttc cgggccgctg
      181 gtactgtccg cgtccgccca catggcagag ctggccccgg aggatgtgga gctattgctg
      241 gatctgaaac ccggcctgac tgccgaacag ctggacgcac ggctcctgcg ggagctggac
      301 gcttccggca aacggcagct gcacaccctt ctgaccacgc tgtatcccac cagtctggcg
      361 gaggaaatgc ctgcgctctg cggcatcccc gcgcaaacgc ccgcctgcga gatcacccgg
      421 gagcagcgcg cccggctggt caaagcgggc aaagaactgc cccttcccgt gaacggcacc
      481 cggcccctga ctgaagcggt ggtcacccgg ggcggcgtca gcgtgaagga gatcgacccc
      541 gccaccatgg aaagcaagaa aattcacggt ctgtacgccg ccggcgagct tctggacgtg
      601 gacgccatga ccggcggctt caacctgcag atcgcctttt ccaccggtcg tttggccgga
      661 atgtccgccg cggaacccga cgctttttaa cacccacaga attggagttt gaacccgcat
      721 gaactacatt gttttggatt tggaatggaa ccagcctgtc agctacgcat ccgccgccta
      781 ccgaaaggtg ggcagcaagc tcctattcga agtcatccag atcggcgcgg ccaaggtgga
      841 tgagacgatg cagatcgtcg attccatctc cattcccatc tgtcccaccc actatctgac
      901 cattcatccc cgagtcaagc gcatgaccgg gctgacgcag gagattctct gcgacgcgcc
      961 tgctttttcc gagggcatgg agcggttcat ggactggtgc ggagacgact gcgtctgcct
     1021 gacctggggc ggcgacgata tcagcgttct gcaacagaat gtggatttct tcaaatttgg
     1081 tcggcccctg ccccggatgt acgatctgca gcggctctac gccgccgaaa tgggacgcag
     1141 cgggcaggta gccctgaaag cggccatgga ggccctcggc atcgacccgg acgagaaccg
     1201 cagctttcac aacgccaagg acgacgccta ctacacggcg ctggtatggc agaagctgcc
     1261 ccatcccgag aaagtactgc agtacgagga gcacgccaag aagctgggtc acagcgagcg
     1321 tcgttcccgg cttcgttttt ccgaaacggt gccttcggtg aaggaagccc ttgccagcga
     1381 caagctgacc gcgccccgct gtcccacctg tcagcagcct actcagctca agacggagct
     1441 gatcccgcag gcggcgggcc gctttgtggc cctcgccgag tgcgcccatc atggcccgct
     1501 ctttgtgcag gcccgtttcg ctcttttgcc agacggccag aaggggatgc gtttgaccat
     1561 ccagcccgcc gaccgccaga cgaaggccta cgttcacacg aaggagctgc aataccagct
     1621 caagcgcaag cggggcgact acgtcaacgt cgatttcgac gatctgcaag acgccctcac
     1681 ctccgacatg cccttcgact aaggggtagt accccttacc ccggactgcg cgcagtgggc
     1741 gcgcagggtg ctcgggggac atgcggcttg gccgtgaagc taccgccgct cggcgacagg
     1801 cctcgcggct cgccggtctg gtgcggctcc
//
LOCUS       DAWAEA010000290         1829 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_8424_length_1829_cov_1.446449, whole genome shotgun sequence.
ACCESSION   DAWAEA010000290 DAWAEA010000000
VERSION     DAWAEA010000290.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8424_length_1829_cov_1.446449"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    479..506
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1298..1516
                     /estimated_length=219
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          335 a          472 c          480 g          295 t
ORIGIN      
        1 cgtacaggcc atatcccgac aaaagcagga acacgtccac gccggaatag ccgtaaaaca
       61 gcaatttttt cagcagcccc tccccgatgg gcagcttcat atggcacagg atcacgccca
      121 aaatcgccag tccctttaac tcatttcgaa aaaggctcaa atcgctcttt cgttccacgc
      181 gtccgcgctc ctctcgttct ccattcgttt ttcgttcttt tccttcctaa tgaaaccaca
      241 aaaccgccaa aaatgcaatc ccggaggggc gctccctttg caaaccggcc aaggcatggt
      301 ataataagcg gaacgtatgc gggcggctgc cgtcaggcgg ccttttcccc cgccatgcgt
      361 ccagaaaagg agcgatgtct catggatcgg gatctttcca ccgggagccg ggaagtggcg
      421 cgggccgcga tcatgatcgc catgacccaa agccgcgagg aagaacagca atgccgaann
      481 nnnnnnnnnn nnnnnnnnnn nnnnnntttt ctctcctgca tggtgcgcat cattgagcgg
      541 gcggtggtcg ccgccaagcg cgagggcctg attgcggagg aacacgagga aggatgcgtg
      601 gccggggccg cccgggaagc gctgagccag atttcctcca aggcgctggg catgagcgtg
      661 ggcggaaaga tcggcgtggc ccgctgcagc ggagacatgg ccgtggcgct ctttttcggc
      721 gtaggtctgg gccatctgga cgaaatctgc atcgggctgg cgcaccgcgc catctgaccg
      781 gctgtcaaac aacatattac gcaaagggat gggacgaacg atgctgctta caatcgctct
      841 ggacggcccg gtcggcgcgg gcaaaagctc cgtggccgac ggcgtggccc aaaagctggg
      901 gatcctgcat ctggatacgg gagccatgta ccgcgcgttt gcgtggaaag cgctccagaa
      961 aggggttgcg ctggaagatg aagccgctct ggaagcgctg acggcgaccg acctgccgga
     1021 agtccgctat gaaaacggcg aacagcacac ctatatggac gggcaggatg tgaccggcct
     1081 gatccgcacg ccggaggtca gcatggccgc cagtaccgtt tccaaggcgg cagcggtgcg
     1141 ccgggccatg gtgaagcggc agcaggagct tgcctcccgc cagagtatgc tgctggacgg
     1201 ccgggatatc ggcacccggg ttctgccgga cgccacggtg aagttttacc tgaccgcctc
     1261 gccggagatt cgcgcccgcc gccgcttcga cgaaatgnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnntcgc ctggcttctg tgccatattt tcatgcctgt cacctatcat
     1561 ggactggaaa atgcgcagga ggacgcgccc tacatcttga tttccaacca caacagcatg
     1621 gtggatccca ttctgatcgc atggggctgc ttccgctacc agattcattt tctgggcaaa
     1681 aaggagctgg aatccaaccc gatcctgcac tggtttttca acaagctgga catgatcccg
     1741 gtggaccggc acaacatgga catgcacgcc ggccgggcct gcctgaagac cctgaaggag
     1801 ggccgggtgc tggggatttt ccccgaagg
//
LOCUS       DAWAEA010000291         1826 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_8442_length_1826_cov_1.684924, whole genome shotgun sequence.
ACCESSION   DAWAEA010000291 DAWAEA010000000
VERSION     DAWAEA010000291.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8442_length_1826_cov_1.684924"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1034..1152
                     /estimated_length=119
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          355 a          514 c          513 g          325 t
ORIGIN      
        1 cacctatacg ttcctgatcg agaactacgg caacgaagcc gcggaagaaa cggatgcggc
       61 gatcgtcgcc gacgtgttca acccgattct gcagaatctc tccgtcacct tcaacggaac
      121 cgcatggact gccgggacgg attacaccta tgacgcgacc accggccgtt tcgccaccgt
      181 ggccggaaag gtgacggttc ccgccgccac gtatacgcag gacgcggcga ccggcgtctg
      241 gacgaccgcg cccggctcca gcaccctgac cgtcaccggc accctgtaag ccaaagacgc
      301 ctgcgccccg ccgcgttttt tctttgcggg aagatgacaa tagagtacca atttgcaaat
      361 atgggttgac aagcctttcc ttggatgact ataatcaaaa tgcgctaaca tggctcttaa
      421 gggccttgaa gtatcgcaat agaggagagg ttatcatgaa cattccggaa atggaagcgg
      481 tctgtaagca ggtacggcgg gacattatca ccatgaccgc cgctgccggt tcgggccatc
      541 ccggcggctc cctgtccgcc acggagattt tggtcgccct gtattatgat ttgatgaagg
      601 tggatcccaa gaaccctaac gaccctgccc gcgaccgtct ggtgctgtcc aagggccacg
      661 ccgcccctgc gctgtacgcc gtgctgggcg aaaagggctt cttcgacaag aaggagtttg
      721 agggcttccg gcagctgcac aatattctgc agggccaccc cgacaagaaa aagtgccccg
      781 gcgtggacgc ttccaccggc tctctgggtc agggcgcgtc cattgcggtc ggcatggcgc
      841 tgggcgcgaa gcacaccggc aacccctgcc acgtgtacgc cgtgctgggc gacggcgaac
      901 tgcaggaagg catcgtttgg gaagcgggca tgtccgcggc tcactataag ctggacaacc
      961 tgaccttcat cgtggataac aacggtctgc agatcgacgg caccaacgac caggttatgg
     1021 gtctgggcaa tatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnacgataag atcgtcgtgc tggacgccga cctggcccag gcgactcaga
     1201 cctgcatttt ccagaaggcg ttcccggagc gtcactttga tctgggcatt gcggaatcca
     1261 atatggtgga cgtggccaac ggcatgagct gcatgggcct gatcctcttc tgctccacct
     1321 tcgctgtgtt tgcgggccgc aactttgagc agattcgcaa cggcgtgtgc tatcctcata
     1381 acaacgtgaa gttcgccttc acccatgcgg gcgtgaccgt gggcgaggac ggcggcaccc
     1441 atcaggctat cgaggatatc gccctcatgc gcgttctgcc gggcatgacc gtgatcgtcc
     1501 cctgcgatgc caacgagacc tacaaggccg tggaagcggc tgccgcgatc gacggccccg
     1561 tgtacctgcg cctggcccgt ctggcgacca ccgtcttcga tcccatgccc ttcgaagtgg
     1621 gcaagggcaa cgtcatgcgg gacggcaagg acgcagtcat ctttgcctgt ggcctgatgg
     1681 tggaacagag cctgatggcg gcggaactgc tcaaggaaca gggcaaggat gtggcggtcg
     1741 tgaacctgca caccatcaag ccgctggatc gggagctggt ggcccgttac gctcaaaagt
     1801 gcaagaacgt ctttacggcg gcagaa
//
LOCUS       DAWAEA010000292         1819 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_8468_length_1819_cov_1.403628, whole genome shotgun sequence.
ACCESSION   DAWAEA010000292 DAWAEA010000000
VERSION     DAWAEA010000292.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8468_length_1819_cov_1.403628"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          366 a          550 c          485 g          418 t
ORIGIN      
        1 tagaccgaaa tcggtgtaga gcttggcggt agagtcgtga taattgcccg tgcctagatg
       61 gagcgtgcgc ttccagcccg cgtccgtctc ccgctcgatc agcgtgatct tgctgtggca
      121 cttgagcccc ggaaagccga aactgacccg gcagcccgcc cggcgaagcc gctcgcccca
      181 gaacaggttg ttttcctcgt caaaccgcgc ctgactttca aacagcacgt agacctgctt
      241 gccattctcc gcagcatggg ccagcgccgc cacgatggga ctgttgccgg acacccggta
      301 cagggtctgc tgaatggtgc gcacggaggg ctcctccgcc gcccgctcca ccagatgcac
      361 caccgggtca aagctgtggt acggatggaa cagcaggtag tccttttcct cgatgcggtc
      421 gaaaatatca tcgcccatca actccggcac cgccttgggc ggcatgggcg tgtatttcag
      481 ttcaggccgc ttgaccatgc cgtacacggc catcatcagc ttattcagat ccagcgggcc
      541 cgtgacacgg gagcgctgct cccgctgaac gtccagccgt ttcatcagcc aggtcagcat
      601 ttcctcgctc atgcgttctt ccgcttccag ccgcatcacc tgaccgctgc ggcgcatccc
      661 cagcatttcc cgtacggcgc cttccatgcc ctccggcgta cactccggca gctcgaaatt
      721 ctggttgcgc aggatgcgga acatgccgca atgctccacc gtttcccggg ggaacagagc
      781 gttgacatgg tggcgaacca cgtcctccag gccgatcaga cattgatcgt ccctcgtttc
      841 ggaaaggtcg tagatacgcg gcagcgacgc gggcagcgcc accagcgcga agcggctcct
      901 gcggccccgg ctgcgggaca gcttgatgca cagatgcagc tgcttctggc tgaccgccgc
      961 atcctgcggc agctccttca aaaagggcag aatggagtcg tgaaatagct ccttttcccg
     1021 gtcggactgg gtatggctca gcgaaaacac tgggtacagg cgaatgccgt gcaggtacag
     1081 ttcgctgctg atgccgtcaa aaagcagata ctgctggtta ttctggctca ggatctcttt
     1141 attgaccttc tggtacagtt ctttggaggt caatccgccc agttgttttt tttctgcttt
     1201 tttccctttt gcagcccgaa aaacctttgg atagcgcacc tgcatgaact catccagatt
     1261 ggaggtgacg atcgcaagga atttagccct ttccagcagc ggattcttcg gctgatccgc
     1321 ctccgcctgg acgcgccggt taaactggag ccagctcagc tcccggtttt gaaatgctga
     1381 cgtttgtgcc tttcccatgc attcggcctc ccattgctct tttgcatacg ttagcaattt
     1441 aaaattatac cacaaccgcg cccccgctgt acagagggcg ctttggccgg atgataggaa
     1501 catggacgct ccagatcagc agccattcgg cattgaagcc ctgctgccgc ccgtggtata
     1561 atcagacgac gcggttaaat gagacttacc gcaattcata aggaggaatg gtcaaatgtt
     1621 attcctgctt caactcgtat tatactgtct tctgtatatt ctgctggtga aatgcgccgt
     1681 aagggacagc gggcttcact gcctgtactt ttatccaaag gaatatattg aggaagcgca
     1741 ccgctgcgga attgcggaca aagccgccgt catgaaaagc ggcaagcgct ttatgattcc
     1801 cttctgcatc atcatcttc
//
LOCUS       DAWAEA010000293         1807 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_8520_length_1807_cov_1.888699, whole genome shotgun sequence.
ACCESSION   DAWAEA010000293 DAWAEA010000000
VERSION     DAWAEA010000293.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8520_length_1807_cov_1.888699"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          390 a          587 c          511 g          319 t
ORIGIN      
        1 ggataggaca acgcttgccg cccaccagca tgaagccctt gccgctgacc gcccggtgat
       61 agccgtcgcc atagcccacg gccacggtcg ccacccgaat atcccgatct gccacgaagg
      121 tgcagccgta gcccaccgga tcgcccgcat gaagggtctt gaggcaggaa acctccgtat
      181 cccaacgcag cgccggttca aagggcagct ctgtggggac gggcggatag ccgtacagcg
      241 aaatgccctg ccgcaccatg gcgaaatcgc cctcgggcat cagaagcccc atggcgctgt
      301 tggacacatg ggcgggaatg gacgggtcga agcaggagcg cagttccttg aaccgggcca
      361 gttgctgccg ggtaaaggcg ttgatcccgc cgttttccag ctgttcgtcc gccatgcaga
      421 aatgggtgta aatgccggtg gccttcacat ggggagcatg tttgagagcc tccgccagcg
      481 cttcggcctc ctgaaaagtc cgcagaccga ttcggcccat gccggtgtcc agcttgatgt
      541 ggatcaatgc gtcccggccc tgcttttccg cttctttttc caaaatttcc accatttccg
      601 gggtaaagac ggtctgggtc aggtcgtggc ggacgcattc ctccgccgct tcctccatgg
      661 cgggacccag caccagaatt tcgccggaaa tgccgccttc ccgcagctcc acgccttcct
      721 ccgccagcgc tacggcgaaa tgcttcgcgc ccatggaatg aagggcggtg gcgacgggaa
      781 ccatgccgtg cccgtaggcg tcggccttga cgaccggcat gatctccgtt ttccggggca
      841 aaaggctctt ctgtaaaaga tagttctggc gaatctttcc caaatccacc gtgaccacgt
      901 tgttgcgcag catatttcag actccttatc gcttgctgaa agaaacggcc caaaattttc
      961 cgaaaaaagt ttgcccattt tgcgccggtt tatgcctgtc attttcagca cgaggacaaa
     1021 cgtcccgccg ctgtgaaaat gcaactttca gtataccacg attcaccgct attacaagat
     1081 gcgtttcatg ccggggatgc gccgcaggag ccatgcaccc gccagcgcgg cggcgaacac
     1141 gacaacctcc cagcaaagtg ccgccccgaa tgcgggcatt ttctcgcaca ggggcagaaa
     1201 gagccggttt ttactttcgg cgatcaaaag atcctgtaac agatagaccc cgaaggcgca
     1261 gccccccagc tcggtcagca accgcccgct tttacgggag ccgcaggcgc agcgaaccag
     1321 ctggaacagc gacagcgacg cggcgatcac cggcagggcg ggggccgtcc gctcgtccat
     1381 gaaccagtac atccgcccgg gctccacccg gtcgaattcc agccgggtca gcacccagca
     1441 gacggccagc gacaaagcca gcgagaccgc cgcaaggaga acggaattgc ggcggggctc
     1501 ccggtatttt gccagataat ggcctgcgaa aaacagcccc agataggcgc aggggatcag
     1561 ctgatccaga tatttcggca gcgccatcac cggaacataa tggctcaaaa gcggccacgc
     1621 cgcgccgacg gtaaaggtca gagccatcaa gtactcatag tcccgctttt ccatggccga
     1681 tgccatcctc tgccagaggg gcaacgtcag cagcaggccc agatagagcg gcagatacca
     1741 gaacccgtcc gtcacctcgc cccgccagat cttgccaaac agcgcgccga agtccaccgc
     1801 ccgggtc
//
LOCUS       DAWAEA010000294         1806 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_8522_length_1806_cov_1.684180, whole genome shotgun sequence.
ACCESSION   DAWAEA010000294 DAWAEA010000000
VERSION     DAWAEA010000294.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8522_length_1806_cov_1.684180"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          331 a          552 c          581 g          342 t
ORIGIN      
        1 gtccggggtg caggggtact acccctgccc ggtagtcgag gggggattgg ccggtgtgct
       61 ttttgaagat gcggctgaaa tgggccacgt cgctgtagcc gaccagcgcg gcaatgtcat
      121 ggacgcgaca ggcggggtcg gccaacagct ccttggcgcg ctccacccgc agggtgttga
      181 tgatgtccag aaagctctgc tgcaggtgcc ggttgatgag cttagacagg tgccactgac
      241 tgacgtagac gtggtcggct acgtcgctca gggtcaggtg ctcggtgtag tgctggcgga
      301 tgtagtccat ggccgcccgg gcgatgaaag cgcccgcttc gccggagctt tcggcgggtt
      361 cggcctctgg ctccaaagcg gaaggcgcag ggggagcctc gtccagacgg gccttcatgg
      421 tggaaatggc ctcccggagc tgctccatgt tgctgggctt gagcagatag cggcatacgc
      481 ccagattcag ggccttctgg gcatagtcga agtcccggta ggcggtgagc actgccacct
      541 gcatggccgg aaaggcgctt ttcagcgcgg cgatcatggt cagtccgtcc atgttgggca
      601 tacagatgtc cgtcagcagc aggtcgggct tgacctcgcg gatcagcttc agcccctcct
      661 gtccgtccat ggcgaccccg gccacctcgc agtcgtattc cgcccatggg aaagcccgct
      721 gcaggccctg taaaataatt tgctcgtcat cgaccaaaac aacgcggtac atgatatgat
      781 cgtcctccta ttcctgccgc tgaaagggat gcagggcaaa gacctgccgc catagctcct
      841 cgggcgtgat gcggccgtca gccagcgaag ggatgagccg gaaccagagg gtgcgtgcct
      901 ccgggctcat agcgtcctgc accgggctga gccgttcggg atggctggcc agcatggcgt
      961 ccgcactttc ccgaagcgca ccggtgaagc cgtagccgcc cagcgcccgg gcattatcgc
     1021 cggtagccag ataactgagc agatgcaccg ctgcgtcctt gcgagggctg ttccatgcct
     1081 tgcgggtcag gtaaaagccc atggaaacgc cccggatgat ggcactgcct tccccctgcc
     1141 gggaaggaaa gggcaaaacc accgtggaat ccatgcgatc cgccggcaga ctgttggcaa
     1201 accagctgcc gtcgatctgc atggcggctt tttcctgaag gaacagctgg ctggcttcgg
     1261 aagcggtgat gacctccgtg tccttcggga agaagccctc ccgctccatc tgcagcagca
     1321 gcgccatgcc ctccgtccag ctggcgggcg cttcctcgga agatgccggc cgctggcgat
     1381 gatcctcctc tccgccacag cacaaaaggc aaaactccgc cacataatga ggctgatcgc
     1441 tgagggagac tgctatgggc acaatgccgt tttctcggaa gaccctggcg gcggtttcca
     1501 gcttttccca ggtggtgggc agctccagct cgtaacggtc gaacagatcc ttgttgcaga
     1561 acagaccctc ccagtactcc cggacgggga tggcatagac ggagccatcg gcctcccggc
     1621 acagttcgct ttcgggcagc tgcagttccg ggtaggcggc gttgatctcc gccacggaaa
     1681 cgacccggct taaaatcggc gcgctgtccg ctgtgccgac gaagtagaac agcacgtcgg
     1741 cctcgtttcc ggcggcaaag tccagcagaa cgccggtttt ccattcctcg ttggaagggg
     1801 tggacg
//
LOCUS       DAWAEA010000295         1803 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_8531_length_1803_cov_1.919336, whole genome shotgun sequence.
ACCESSION   DAWAEA010000295 DAWAEA010000000
VERSION     DAWAEA010000295.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8531_length_1803_cov_1.919336"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1514..1523
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          379 a          552 c          563 g          299 t
ORIGIN      
        1 gaggcagaga cacgtacagg aacgccgtcg ccccggagga gccgatggag cggggcatgg
       61 ccagcgcgga caaaagccac gttaccagcc gggccacgaa cgccagcagg aacagcagaa
      121 tgggcagctt cttgtctttc ttcgccagca cggcggcaat gccagccagg atcagcacca
      181 tggtgatcca gctggaaatg ctgaaggact tgaacagctt ctgcatcagg tcttccactg
      241 cggcggcgtt cagttcgccg tccttggctt cttcctgata gatggccttg cccttggaat
      301 cggtgggatg gagcagttct tccggcgcgt ggaaggtcag ggtttccagc ccggagacgt
      361 tcaccgtggc cagcaccttg ttcttaccgg gcttggtgaa ggagacggtg gtgccgggca
      421 gcagcacgaa ggccagcgac accagcgcca gtgccagcgc gatcaccttc aggcggctct
      481 ttttgggaat ggggttaacg aagtacggct cgttggcata acgaacgccc agcacgccgc
      541 agccggtgct ggccagggtc agcagcgtca gcagcatggg cgtgagcgtc aggctgccca
      601 gacccagctg gcacagctga cgggtggcgg tggagaacat atctccgctg ccaaggctca
      661 taaggcccat caccgtagcg cccacgcaga tcagcgccag cacgctcagg ctcagcggga
      721 accagcggtc caccttgggc aggaaggcca gcagcgccgt cagcgccagc aggatcatcg
      781 ccgcgatcag ataggcgttg ggcgcggcgg tggaggaacc gatggtaaaa atgccctgaa
      841 tgttattggc gtcgttggaa tagggcacca gcacttcgat atcgccggtc agcacgctgc
      901 cgtaaacgcc cttttccaca gcctcggccc ggaggttcgg ctcgtttcca agcatttcct
      961 tccacagcgg cgtggagcgg ttcatggccg ccgcgtctgc cgggtcagcc gtgatgcttt
     1021 ccggcacgga caccacatga gagggcagca gcaggcacag catggccaga atagccagca
     1081 cgccgcttgc cagccggaac gtccgatcgg tgatcttcat ggccggagct tccttcagtc
     1141 cgacgaagcc cagaaccacc ataatcagac cgccgatggc cggggcgtag atccacgcct
     1201 gattttccag aagcagggta aagaggatcg acccgcagat gttggtcacc tgcagggaga
     1261 aggtaaaggt cagggcgatg gaaaggaagg acagcacaat ggacacgcgc gtccaccgct
     1321 cccccgccac cagcgccgca gcggccagcg ccagcagcac cagggaggga tagatattgt
     1381 cgcggtagac gcgcagcgta ttttccgccc aggaggccgg ggtatacagc gtgacggcga
     1441 atacgaacag ccgtccccag tttgtgcgca tcacgggctt cagcgccgcc agcagcacca
     1501 gcacgactat ctcnnnnnnn nnncgccgca gcggccagtg ccagcagcac aaggcccgcg
     1561 atcagcatcc agttgtcaaa ggtgatcagc ccaagctggg gaacctccgc tgtgggaagg
     1621 cagtccgccc ctttgagcat aatgtctccc acggagaccg ttttgggaaa cagcgccgcc
     1681 gtttcctctc ccagctggct atggacatac accttggccg cgggaaggaa cagcgtggct
     1741 agaacgatgg caaggacgat gataccggcc cagcgctttt tcaattccga ccccttcttt
     1801 ctt
//
LOCUS       DAWAEA010000296         1788 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_8617_length_1788_cov_1.388921, whole genome shotgun sequence.
ACCESSION   DAWAEA010000296 DAWAEA010000000
VERSION     DAWAEA010000296.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8617_length_1788_cov_1.388921"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    855..864
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          385 a          497 c          537 g          359 t
ORIGIN      
        1 cacagccgtt ccagccgccg gagcacattg gccagatgac tgccgaacac gccgcggtag
       61 gtgtgaattt cgtcgatgac aatgtattgc agattttcaa acaacttcac ccacttggta
      121 tggtggggca gaatattggc gtggagcata tccgggttgg tgaccacgat atggcccgcc
      181 tgacggatgg atttccgggc ggcgttttgg gtatcgccgt cgtaggtgta ggccttgata
      241 tccatatcaa gagcctggat catatcatac agcccgctga cctgatcggc ggaaagcgcc
      301 ttggtaggga acagatacag cgctcgtgcg tcctcgtttt tcaaaatggc gctcagaacg
      361 ggcagattat agcacagggt cttgccgctg gcggtggggg taacgacgac gaagtctttc
      421 ttctcctgcg ccagttcaaa gctttgggcc tgatgggtat acagccggta aatgccccgt
      481 ttgcgcagca cttcctgaat gcggggatcc atgctttcgg gaaaatcagc gtattttccc
      541 tccgtagcgg gcagcgtgcg ccaatgggtg acacaactca taaatttggg attctgacga
      601 agctggccca acagctgatc cagattcaca ggaaggcccc ctttcccgaa aaaacataga
      661 gaagcgaaca aacgtgcgcc ccgctggtat tatagcaggt gcggggccgg aatgacaagg
      721 ggaaaatgca gcgcttgacc agcttcgccg aaaagggtat gataaaggag gaaacctttg
      781 atttttcatg gatataggag caaagcatgg cctatcaagc attatatcgc cgctggcggc
      841 ctcagacctt tgaannnnnn nnnnaccctg cggcatcaga tcgaaacggg gcagatcgcc
      901 cacgcctacc tgttctgcgg ctgccgcggc acgggcaaaa ccaccatggc caagctgatg
      961 agccgggcca tcaactgtct gaatcccaac cacggcgacc cctgcggcca gtgtgaggtc
     1021 tgccgggcca ttcagacgga aagcaccatg gacgtgttgg aactggacgc ggcctccaac
     1081 aacagcgtgg ataatatccg cgaactgctg gagcaggtgc gctatcccgc ccaaatggga
     1141 aaatacaagg tctatatcat cgacgaagtt cacatgctgt ccactgccgc cttcaacgcg
     1201 cttctgaaaa cgctggagga gcccccggcc cacgtggtct ttattctggc cactaccgag
     1261 ccacagaagc tcccggccac catcctgtcc cgggtgcagc ggtacgactt tgggcgtatt
     1321 ccgtcggaat tgatgatcgg ccgcatgaag gaagcgctgg acgagcttca tatgaccgct
     1381 gaacccgagg cgctgcagct ggtggcgcgt gccgccgagg gcgctatgcg cgacgcgttc
     1441 agcattctgg atatgtgcgt ggctgcatcg ccgaccggga cgcggcgggc gttctgcgca
     1501 aggtggacga gctgatgcgc tccggccggg agccacaggt gtttctcaag gatctgagca
     1561 gccattgccg gatgctgatg accgtgcagg cggtgaagga gaacgccgcc gcgctgctgg
     1621 atgtgaccac cgaggacgag cagcgccttc gggaacaggc ggagcgcttt tcgtttcatc
     1681 ggcttttgcg ggtgctggac ggctttcttc atgccgaggg agagcttcgc ttcgcctcca
     1741 cgcccagaat cggactggaa atcgcggccc ttcacgcctg tgaagccc
//
LOCUS       DAWAEA010000297         1786 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_8627_length_1786_cov_2.634315, whole genome shotgun sequence.
ACCESSION   DAWAEA010000297 DAWAEA010000000
VERSION     DAWAEA010000297.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8627_length_1786_cov_2.634315"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          322 a          598 c          552 g          314 t
ORIGIN      
        1 gctcacagcc atccgccgcc cggcgctgtt cagcggttcc cgctgatccc gcccgccgct
       61 ttccagcacc cgttcataaa gccgggatgg actcaaaata tcaatataaa aggtgccttc
      121 cagctgcagc tggcgaaaaa tttcccgttc ggtctgaagg gtcagctgct cgggcaccag
      181 cagcaccaca cttttttcct gccggttcag ccgacccagt tcttcgatca acggcgcaag
      241 cagtcgatgc ggccgggccg tcaggatgcg aaccttctcc gccacaggca aacctccttt
      301 caaaaatcaa aagctctggc attccgccgt tcttcatgat atgatagcac cacggaaagg
      361 aagggacaag ccatgtcaga tcccattgtg ggcattgcca ccgccgccgg ggaaggcggc
      421 atcgccatcg tccgcatcag cggagacggt gcggcggact tattccagca ggtgttccgg
      481 cctgcgggca agcgccgtcc gccctatgaa agtcaccggt tgatgtatgg ccacgcggtg
      541 gacggcgccg aaatgataga cgaatgtatg ggcgtggtca tgtacgcgcc ccacacctac
      601 acccgagagg acgtgtgcga gctgcacacc cacgggggcc gggtggcggc ggcgctggtg
      661 atgaaggctc tgatccgctt gggggcccgg cccgccgaag cgggcgaatt tacacgccgt
      721 gcctttatga acggccgcat cgatctgtct caggcagagg cggtgatggg ggtgatctcc
      781 gcccaatccg ccgcggcgct gcgggcgcag gaggcgcagc tggagggcgg ccccagccgt
      841 tttatccgtc aggcacagga agacctgacc cgcctgttgg ccgggctgga agcccacatc
      901 gactacccgg acgagatcga cgaagccgaa gcgctggacg gtttgaccgg cgggctgaac
      961 gccctgatcc tccgtctgga aagcgccgtg gacgagcgca gcgcccgcat tctgcgggaa
     1021 gggctgcgtg tagccctctg cggccgcccc aacgcgggca aaagcacgct attcaacgcc
     1081 tttttgggcg aagaccgagc catcgtcacc agcattcccg gcaccacccg ggacgtgctg
     1141 gaggaaagct ttccgctgga cggggtcaac gtgatcctgt ccgacaccgc cggtctccgg
     1201 gacagtgacg acgaggtgga gcgcatcggc gtggagcggg cccgcaaggc gctgaaccgt
     1261 gcggacgccg cgctgctgtt ggtagacgct tcccagcgcc tttccccgga ggacgaggcg
     1321 ctgctgcgcc agcccattcc ctgcccctgc gccattctgc tgaacaagca ggatgccggt
     1381 gaaacggttc gtccggccga tctggcctct cttgccgcag gccgtcccat tttcccgatc
     1441 tccgccctga ccggcgaagg gcttacccct gttcgggatt ttcttcgcgg cttcctaagc
     1501 cagcccaacc aatggcagct ggtgcaggag cggcacatgg ctctggcccg gcaggcggcg
     1561 gaccgtctcc gtcaggctca atccagcctt tccaccggcg cacccctcga ccttgccgcc
     1621 atcgacctgc acgaagccct ttatcttctg ggccgcatca ctggcgacag cgtagacgag
     1681 cagcttctcg acgacatctt ctcccgcttc tgcgtcggca aataggcagg gcaggggtag
     1741 tacccctgca ccccggactg cgcgcctatg gcgcgcaggg tactcg
//
LOCUS       DAWAEA010000298         1785 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_8630_length_1785_cov_1.310405, whole genome shotgun sequence.
ACCESSION   DAWAEA010000298 DAWAEA010000000
VERSION     DAWAEA010000298.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8630_length_1785_cov_1.310405"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    438..522
                     /estimated_length=85
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1139..1191
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          423 a          443 c          440 g          341 t
ORIGIN      
        1 ccgccccgtc ccccagacgg aacgcgccgt cctgcctcca gagccgggca gagcctaccg
       61 cccggtcctc ccgaaatacg attacctgct gggcctgatc gtccagcgcg tcccgaccgc
      121 ggcccaaaac cgcccgccgg atgctcagcg gcagctcgat gttgctgccc accgggtacc
      181 atctgccctg taccatctca aaaaccccct tcatcatttg gaaaggaaat ggattaccat
      241 ggaacatgaa aagatccttc gcatcaatga actggccaaa atcaaaaagg aacgcagcct
      301 gactgccgac gaagaggaag aacgcaaggc ccttcatcag gaatatatcg cgggttttcg
      361 ccagaacatg gaagccgtgc ttcaaaatgt gcgcgtcaag cagccggacg gcagcctgac
      421 gccgctgaaa aagaaagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnctcaagga ttttatttgt
      541 gaaaaagccc ttgtatcttt cgggcacttc gggtataata aagacgaacc aaggggtaag
      601 aagtaagaga cgagatcaaa acagaaagta ggcgattccg ttggacagca aggatcagat
      661 tttgggcggg aatcccgccg aggaagaaga agcccgcgat ctcatcgaag gtgtcgatga
      721 agacggtaac aatgttctgc tggaagtggt tcgctatttc ttctacaacg gcgaagaata
      781 cgttgtattg ggcgatgctc acgatggctg ctgcgactgt ggatgcgaac attgtgacca
      841 tgatcatgac gaggacgaag aagaggaagc cgtcaacctg tatatcatga aggttgtgga
      901 atccgaagag gacggcgagg aaatggaatc cttcgttccc gtggaggatg acgacctgct
      961 cgaaaagctc attgaggtcg ttcaggctga ctttgaagcc gacgacgaac tggacgatta
     1021 acggtttttg tttcccccat accagttttc tttcccctgt tttttccatt caaccggctg
     1081 ctgccgtaac gatagatttt tggagaagaa agcgggcagg tcacaaaact gaataatcnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtgcggtat
     1201 cgtccgcgcg ggttctcttt ctctaaaaag tctgttgcaa tgaaagaagg cccgtccatg
     1261 cagtgcgaac tttccccgct ccatacccgc cagccctatc gtttcgtggt cattttgtcc
     1321 ttctgcggcg aacagttggt gctgagccgc cataaacaac gcacgaccta tgaaacgcag
     1381 ggcggtcaca tagaacccgg tgaaacgccg gagcaggccg cccgccggga actctgggag
     1441 gaatctggcg cggcagaata cgatctggtt cccctgtgcg actactgggc cggagacgaa
     1501 cacggctggg ctaccgggca ggtattcgtg gccgatgtgc ggcgctttga agccctcccg
     1561 gaaagcgaaa tggcggaaat ttgttttttt gacacgttgc cggagaacct cacctatcct
     1621 gatcttactc ccaaattcta tgaatttgcc ctgaaaaagc gctgcaatgc taccccaaag
     1681 ccatagaaaa acgcccgatt tgccaacgca aatcgggcgt tctatgaacc atacataaat
     1741 ccttatccgc gggcagcctc ggcaaacgtc gcgaacaacc ggcgg
//
LOCUS       DAWAEA010000299         1782 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_8637_length_1782_cov_1.924146, whole genome shotgun sequence.
ACCESSION   DAWAEA010000299 DAWAEA010000000
VERSION     DAWAEA010000299.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8637_length_1782_cov_1.924146"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          395 a          570 c          528 g          289 t
ORIGIN      
        1 gccccagacg gtggaagaac tgtggcaggc catccagaat tacggcggtc tgaccatccc
       61 cgcaggcctg acgcaggagc aggtggatgc catccgcgcc caggccaacg atctgctggc
      121 ggccatcgat gaaatccccg cagacaccct gccggaagaa caacagcagc gcttgcagga
      181 ggcccggacg tatatcgccg ccgtggctga tgctgccgac cctgccgcca tgcagacggc
      241 tttgcagaat gctgtcgatt gggcgcacca gttcgccccc gaactgcccg atgacatccg
      301 ggacaagctg aacgccgctg tcgaagcctg gtgggccagc ctgcccgagg acagcaaagc
      361 ccagctggaa gaactggttt ccggcgtggg tcaactgacc cagtaccggt tgggcctgct
      421 gcaatacgaa cagggagcgg ctcagctggc tgccgcccgt gccgagctgg aagccaacgc
      481 ccccaaactg gccaccgcca aggcccagct ggaggatggc tggaagcagt actatgacgg
      541 cctggcccag tacgaggacg gcaaaaagca gctggcggct gcccgcgccc agctggagga
      601 tggctgggcc accctgcagg acaaaaaggt gcagatggcc gatgcccgcc gccagatcga
      661 cgacgccaaa ggccagctga gcgacgcccg caaaaagctg gatgacgcca aaacaaccat
      721 tgccgaaaac ctgcaaaaac tacgggacgg agaaatagaa tatgaggacg ccaaggtcga
      781 agccgaaaag gccctggccg atgcccgcgc gcaaatcgag gacggagaag ccgccctgga
      841 cgatgtggag taccccacct ggtacgtctg ggaccgcagc aaaaacgtca gctatgcgtc
      901 ctttaccgcc aatgtggata agctcaccgc cattaccacc atctttccgg tattcttctt
      961 cctggtggcg gcactggtgg tatccaccac catgacccgc atggtcgagg aagaacgcct
     1021 gcagatcggc acgctgaaag ccctgggcta ttcgggccca gccatcatgc aaaagtacct
     1081 gatctacgcc ttcacggcgg ctgcagcggg cacagtagcc ggactggccg tcggcttcaa
     1141 ggctttcccg tccatcatct ggtccgccta tgaaatgatg tattatatgc cgtctattgc
     1201 caccccctgg cggttgagcc aggcgttgct tgcgggcggc acccttatcc tgctcaccgt
     1261 gggcatcacg gcgctggcct gccgcaccac cttgcaggag aacccggcgg ccctcatgct
     1321 gccccgcgcc cccaaggccg gcaagcgcat tttgctggaa cgcatcaccc cgctgtggcg
     1381 gcggctgccc ttcagctgga aggttacacc aagcagtacg gcgacattta ccactacgac
     1441 ctgatgacca tcgtcaccaa gcaggaggcc accgaaagcg gccctgttta cggctacctt
     1501 tataataagg agaatgtcac cgattcgctg actgtcgcca tggaatccac ccggcaggat
     1561 ggccccaacg gtgagatgga cgtctacctc atggttcccc aggacgtgga caagtttgct
     1621 gagtttgctg acctgcacga gcgcctctcc cgcaaggcgg tgccgctggg gcagcagggc
     1681 gtggtcgtga ccgaaaagat ggccaagacc ctgggtgtaa aggcaggtga caccattacc
     1741 ctgactaaca gcgacgataa gacagctgac ttcactgtca gc
//
LOCUS       DAWAEA010000300         1759 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_8741_length_1759_cov_1.282277, whole genome shotgun sequence.
ACCESSION   DAWAEA010000300 DAWAEA010000000
VERSION     DAWAEA010000300.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8741_length_1759_cov_1.282277"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1294..1303
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          327 a          509 c          529 g          384 t
ORIGIN      
        1 ttctactctg acagctgggc cgaagtcggc gatgtggcta ccaccctggg tgccaacaac
       61 gacgctgtgg gcctgcccac cgagacctgg tccctgacca agttcaccgt ggaagagtac
      121 gaagcgctgc tggcctccat caaggacggc accgttgcca tcgacaacac tgcgctgaac
      181 aacgacgaag tcgtgaacgc tggtctggaa aacgtgacca tcatctacga ataagccata
      241 gggcaaattc aaaaaggagg agcgaaagct cctccttttt tgataaaaat gaaaatacaa
      301 aagccgcccg gggtttcccg gacggctttc gcctgtctga aattacttca catcccaggg
      361 gttgggcacg aaatcgccgc cgcggcagaa tttcaggtac ttcagcgcgt gcatctggct
      421 ggtcatttcc caatcgccgg cgtataccgg gtcgtggtac ccttcgatgc agacgtcgcc
      481 ctcgtagccg ttttcatgca gaatggagaa aacgtcccgc cagttggtgt cgccgaagcc
      541 gggcgtgcgc tcgggggcga aatccttggc ggcgaagatg ccgtggtggc tgaccgcatc
      601 ccagtccaca gaggtgtcct tgccgtggag atggacgatc ttcttggccc acacccgcag
      661 ctgggcgatg gggtcgatca gctggatcag ctgatgggca ggctcccatt ccagacccag
      721 tgcgtcggag ggaaccgcgt cgaacatcag ctcccatgcg tcggggttga tgccgatatt
      781 gcaggtggcg cttcacgccc cggtcttcgg cgcgcttggc cagctcggaa aagacttcct
      841 tgaaccgcgg aatggccgcg tcgatgctct ggccttccag cgcgcccgcg aaggtcgcca
      901 cggtcttggc cccgaagaga tgggcgttgt cgatggcgta ttccagatcg tgcagctggg
      961 attcgttctg cagggcgttg cagtagtagc ctaccgtgga aacataggtg cccgtgcctt
     1021 ccagcattcc catcaccttg ggggccaatt ccttcaggtc gatgccgctg taggtcatgt
     1081 ggaagttgat ggcaaagcac tcaaagccct tgtccttcat gccggggagc cactgctccg
     1141 cctgctgtcc gggaatacag gtaccgattt tgatctgttc cataatggtt ccccttgcgt
     1201 atggttttgt ggggttcagt ccagcttcag ttcgccgttc tcaaagtaga cgggacggtt
     1261 ggtctcggcg gaacggtaga tggcttccaa aatnnnnnnn nnngctcggg cttggtgacc
     1321 agaggcttgg aatcgtccag cacgtgagcg atccatgcgc gagcttccag ctccgccggg
     1381 tcgccgccgg tggtgccgtc gtagaaggct acgccgcctg ctttcaggtc aggctcggtg
     1441 acgaaggtct tggagtactt ttcgccgttg atgcgcaggc cgccgtcctt gcccagcata
     1501 tccgcgccgc ccttgtcgcc gcagaggatg gtcttggcct cgttcacatc cagcgtgttc
     1561 agcgcccagc tggcttccag atagatggtc gcgccgttct tcatcttaat gaagccgaag
     1621 gcggaatcct ccaccgtgaa ctttttggga tcccaggggc cccacgcatt ggcggagttt
     1681 tcgtgatccg ccagcttgcg gaaggtggag ccggtcacca tggcgggctc gtagttgttc
     1741 atttcccaca gggtcagat
//
LOCUS       DAWAEA010000301         1757 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_8749_length_1757_cov_1.599295, whole genome shotgun sequence.
ACCESSION   DAWAEA010000301 DAWAEA010000000
VERSION     DAWAEA010000301.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8749_length_1757_cov_1.599295"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    717..726
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1357..1366
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          377 a          473 c          556 g          331 t
ORIGIN      
        1 aggtgcagga gatcctgcag aagtacaacg aattgcagga catcattgcc attctgggca
       61 tggatgaact gggcgaggaa gaccgccagg tggtgctccg cgcccgccgc atccagcgct
      121 tcctgtccca gcccttccat gtggccgaaa agttcaccgg cattccgggc cgctacgtgc
      181 cccgggagga gacgattcga ggctttgccc gcatcgtgga cggcgagctg gatcaggtgc
      241 ccgaagccgc gttctacaac gtgggttcca ttgacgaagt gctccagaaa gcgaaggaga
      301 ctgcggaatg aacacatttc gtctgcatat tctcgccgcc gaccgggtgt tcttcgaggg
      361 cgactgcacc tcgctgatcg tgccgacggt acatggccag tacggcgttc aggcccgtca
      421 cagcaacatg atcgccgctc tggtgcccgg ccagctcacc tttcgctgcc cggacgcgcc
      481 ggaacggctg gccgctgttt ccgccggaat gatcaaggtg gaggataacg acgtgctggt
      541 tctggtggac gcggccgagc gcccggagga tatcgacgcg atccgcgccc gccagcatgg
      601 acagctccgc gcaggccacg cccgtgccgc ggtcgttgtg cggatggacg gagatgattg
      661 ccgcgtcgcg gttgggcaga tggcggatga aatattcgat ctggtcggcg taggtgnnnn
      721 nnnnnnccac cttggcccgt gccctgaacc gcctgcgggt caaggaacac cgggaactgt
      781 aaaaaaatat ccctgctttt cctgaaaacg ggaaagtggg gatgtttttg cctgtaggct
      841 gcgcagaatg aaaaaagcag caggaaagta caaggtaaag cacgatcttc caattgttcc
      901 cacccggagg atatgataca atgaacgcac aaacaaccaa tgggaggaaa atcagcatga
      961 cgaccgaaga attgcgggcg ttgatccgcg atatgagcct gcaggagaaa atcgcgcagg
     1021 taagccagat cggcatggag agcttttttg ccaattccac tgagcctacg gggccgatgc
     1081 gaaagtttca tttgagccgg gagcagatgc ttcaggcggg cagcctgatc ggtgccttcg
     1141 gcatcgacga gcaacagcag caggaaattc ggcgactggt gggggaaacg aagcaccgta
     1201 tcccgccgct gatcatgtgc gacgtgatcc acggaatgcg cagcatcttc ccgatcccgc
     1261 tgggtatggg cgcttccttc tctgagaaaa acgcggagat catggcccgc accagcgccg
     1321 aagaagccac ggctcggggc attcacgtga ccttcgnnnn nnnnnnattt cgcggcctat
     1381 gggctggtgg aggcggggca ggattacggc catgtggacg tgagccgggt ggaaatgtac
     1441 aatacctatc ttccgccctt taaggccgcg ctggatgctg gctgcgatat gctgatgccc
     1501 gcctttacgc cggtggaccg ggtgcccgcc acggccaacc gctggctgat cggtcaggtg
     1561 ctgcggcagc agtgggcgtg ggacggcatg attatcactg actggggttc catcgacatg
     1621 atgctggtgc aggcatttgc cgaaagtatg gaagaagctg tggaacaggc cttcaacaac
     1681 accgttgata tggacatgat gagctacgcc tatttcggct gtctggaaaa gctgattcga
     1741 gagggcaagg tcaagga
//
LOCUS       DAWAEA010000302         1754 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_8762_length_1754_cov_2.066510, whole genome shotgun sequence.
ACCESSION   DAWAEA010000302 DAWAEA010000000
VERSION     DAWAEA010000302.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8762_length_1754_cov_2.066510"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    597..606
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          330 a          540 c          541 g          333 t
ORIGIN      
        1 gctggcaggt gtcgatcaga tcatccagcc atgcaatggc ccagtcccga atttagtccc
       61 gggccagatt tagcaccagc tgatgtcgct gctggatgcg gggccgggca gggtcgtgaa
      121 tgacccagtc ggggtgcgcc cgatacaggt cgctgtccgg gttgaccatt tccggctcca
      181 cccacagtcc gaagagcatc cccttgtcgt gacaggcctt ggagatagcc tgcagcccgt
      241 ggggaaaacg ctccgggtcg gcaaaccagt cgcccagacc ggccttgtcg tcggtacgct
      301 tgggcatcca gccgtcgtcg atgacgaaca gctccacgcc cagcggcgca cacttgtcga
      361 tcagcgccag acagttgtcc tcccgcacgt caaaggcata gggataccac gaattgtaga
      421 tgacgggccg cacggcgtgg gccttgtcgg tcttattgcc ccggggcatc aggtggtcga
      481 actgccagtc gtagaagacc tcgctcatgc gctcgaagcc ccggttcgag aagcctccgg
      541 tgaactgggg cgtttcaaag cactcacccg gcttcaaaag gtacttggtg tcgaaannnn
      601 nnnnnngtgt cgaaactgtt cacgccgccg gtgacagtga ccattttgcc gaactgccgc
      661 tccacggtga tatcgaaatt gccgctccaa tggagcacgc cgaagaacac ctgccccgtt
      721 gtttcggtgg ccgcgccgtc ctgatccagc gcaaagaagg ggatctgctg agcggcggcg
      781 caggtgatgc ggttgttttg cagcacggtg cgggcctggg tcagcatcag ctgatttttg
      841 gtgtactcgc agccccagtt gccgctcaga tgggtcagcc gatagtccca accaaagggc
      901 agctgccacg cggggctctt catctgttcc agctccagcg cgccctcctc gccgttgcgg
      961 atgaccagcc atttgctcag cagcggcaga tcggcccagc cccggtaatg cagctccacc
     1021 gtgacggcat aggccgcgtc ccgggcgacg acggtcaggt ggtcgccgtc gatcaaatgg
     1081 ctgtcgtaca aaagacgcag accccggctg ccgtccgggt ggcgcgccca cagcaccggg
     1141 tcgccgtaat cgctgggctc catggtgggc agctcggcca cgccgtcttt gcgcgcgtcc
     1201 acgcccttgg agggctcgta ggccgccagc attgtctcgg ccagcggcag cagagaatcg
     1261 tcgctgctca gtgacgatcc ccagtacagg tggcgcagcc gtccatcggc agcaataccg
     1321 aaggcgtagg acatttcaga agtccgaagg tgaaagagcc ggtgagcttc gtcaaagtga
     1381 atacgcatgg ggaaaccctc cttgtgaagt tttcattcgc tcaagagaaa cgttcttctt
     1441 ttaattgacg gtctgccaca ggttttgcag gggaaccggc cgggcttcgg gctgctcctg
     1501 cccgtccagc agcgggtaca tccattccgc aaagcgccgg aaagcctccg ccgccaacgg
     1561 atagggacgg ttgcatacgt cgatcaggcc gatctggtag cattcgccgt cgaagcggcc
     1621 cagatagggc tggtcgctgt attcgaaata gtgaacgccg gtcaggtggg gctcctgcgt
     1681 gctctgctcc agataatacc gcagcgcctc ggcccgctgg gactgcgtgc ccgtatggta
     1741 cagaccccag gaat
//
LOCUS       DAWAEA010000303         1741 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_8834_length_1741_cov_1.547450, whole genome shotgun sequence.
ACCESSION   DAWAEA010000303 DAWAEA010000000
VERSION     DAWAEA010000303.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8834_length_1741_cov_1.547450"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    324..338
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1041..1065
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          339 a          489 c          552 g          321 t
ORIGIN      
        1 gaggaactgc gcaccggcgg tgtgctctct cccacggagg tgggcacagc catcatggag
       61 accagcggca aactgagcgt attccccacc tcccaaaagc ggcctgtcac cccggaggac
      121 atgaagctga aaaccggcaa cgaaggcatt ccgctgacgc tgatccttga cggccagctg
      181 gaacagaaca atctgcccct gctgggcaag gacgagagct ggctgcggca gcatctgggc
      241 agcatgggct tttccgagat tcggcaggtg cttctgtgct acatcgatac ggacggcggg
      301 atgagcgtcc acggtttccg gggnnnnnnn nnnnnnnntg ctttcatccg gggaggtgag
      361 gtggtagtgc gccgttccgt gatcctgatc tccgtcattc tgacgctttg cattgcgctg
      421 ggcatgtgga tggacgtttc ccagcagcgg gtcgcccggg aatatctgga ttccgtccgt
      481 ctgatccgcg atctggcaga cagcggccag caggcgcagg cctacgatga gcagcgctat
      541 cttcacgccc agtggcagca cgacgctgtc tggctcaatt accttatcag ccaccaccac
      601 acccgggccg tcagcacggc catggtggag ctgtccacag cgctggaacg ccagtgggac
      661 actgagacct ttcaggcact ggacgctttg gaggacgcgc tgcgcgacgt ggagcagagc
      721 gatttccctt attgggaaaa cattctgtag agcctttctt ccacttgaca gggcctgttc
      781 aggcggggaa actgaaagaa acttcttttg aattttcggc tgtacggagg aaaaagcatg
      841 gagacctacc aagcggaagt tctggtcaac gagcggtttc atctttcgga ggggcctgtc
      901 tgggacgcgg aaaccggcgt tctgcacttt gtggacatca agggctgcac ggtgaacacc
      961 tatcggctgt cggacggaca gcgcaccagc acggaggtca accagaacgt gggctgcttc
     1021 gccctgcgcc gtcagggcgg nnnnnnnnnn nnnnnnnnnn nnnnntttta ggactgacgg
     1081 cgggcgttta cctgcgcagc gaggacggac ggcggatcga gaagctgacg gagccggtct
     1141 acgaccccat gacccgttcc aacgacggca aatgcgaccc gcaaggccgt ttctggtgcg
     1201 gcacctccga tctggtgaag ggcgaaaagc ggggcaagct ctacctgctg cccggcgacg
     1261 gccgctgcat cgaaatgctg gaccggctgg aaatttccaa cggtctgggt tttgcgggcg
     1321 acacgctcta ctttatcgac tctgcccggc acagcgtgga cagctaccgg ctggacgagg
     1381 acacgctgac tctgcacgac catcgggtga tcgcccccat cgaggggaac atggtgcccg
     1441 acggcatgac catggacgaa gagggaatgc tctgggtctg ccactggggc ggtgggtttg
     1501 tgggacggta cgatcctcgg gacggccgtc tgctggcccg ggtggagatc cccgcatccc
     1561 agtgctcgtc ctgctgcttt ggcggcccgg atatgcagac gcttttcatc acctgcggag
     1621 cggtcaactg cccggaggag tccgaagccg ggaaggtatt tgcggtgcac ctgccctatc
     1681 gcggggtgga aagctggaaa taccgggggt aaagcatccc cggaaaaccc atacaaaaag
     1741 g
//
LOCUS       DAWAEA010000304         1736 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_8858_length_1736_cov_1.749554, whole genome shotgun sequence.
ACCESSION   DAWAEA010000304 DAWAEA010000000
VERSION     DAWAEA010000304.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8858_length_1736_cov_1.749554"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          353 a          499 c          506 g          378 t
ORIGIN      
        1 cagtgcctcc tgcacctcca ctttttcagc aatttgaggc gcggccattc ccgcgccttt
       61 ttgtgcctgt tttctgttac gcgcccgcgc cgctgcggtc ctgcgcccgg atgcgcccgc
      121 agatcagcgt gcacacatcc ccggcaatct ggatcagccg ggggtcccga agcatggcca
      181 gcttttgatc gaaccttttg ccgatggccc cgatcaggaa gggacagccg atcagccctt
      241 cttcaatggg cgggaaaagc ccggcttcca tggcgtcgga gaaaatcttc gccgcccgga
      301 tcatgccgcc ttcccggcgc agcagaacgg tcacactgcc ccacgggaac cgctcagaca
      361 cctcgaagtc atagttttcc tgagcggggt atgtccattc cgggtgagaa aaacgggagg
      421 tcagccggtc aatgctggac tcgtccagca gctgctcatc cagccatgcg ggctgggcgc
      481 ggaagcagcg ggcaaaggcc gcgaccagcg cccgttccag cctgtccagc gtcaggttcg
      541 ggttcagctc ccgcagagtg accatgcggt tctggatggg agtctcatcc tgcagggacg
      601 ggtccttgcg acgggtgcgg aaatagtgct ccatcatgtc cagcgctgtg ttatacaaca
      661 gcgtaccgtg atgcatggcg acggaaccgg ccttgtagaa agtgttctgg cagaagatac
      721 gtccgcctaa gcgcaggctg cccatggggc ccatctccca cggaaccccg acggacgaca
      781 gggcttcgcc gatcaggtgc agctgtcggc cggcattgaa ctcgcttttc ggcaggatca
      841 ggctgaaatt caatgtaccc atgtcctgat agctggctcc gccccccgac agccgacgaa
      901 cgatctttcc gcccgattcc agaaaggcct ctaccttgca ctcgtaccac gggttctgat
      961 tccgtcccac taccacggcg tggcggttct gggacaaaaa aagaatggcc gtgttttcgg
     1021 gcagggtgtc catcaaatgt ttctcgatcg ccagattgcg aaacacatcg tttccacgcg
     1081 caatcaggca ggcggtgcgt tgtatcattt cccttgctgc ctttccctgt gctgttcttt
     1141 ggcaaatagt cccggcttat atcccggacg aacgattctt gctccagctc agtatagcac
     1201 aaatgaaaga taagagaaag cccctttgca ggagaagcgg aaaatcgctt tttttcgcgg
     1261 gtttggagcg ggaaggttgc tttttccctg acgaaccgtt acaatgggca aaacgcttcc
     1321 gggaggttct atatggatct gtcgattcgc agaatgagga aagaggactt gggctggctt
     1381 tatcgtcttt tgtccgaccc ggaggtcatg cgctggctgg aaccgcccta cagcatggag
     1441 caggcggaaa cattcctgtt ctgtgcggga ttgtcagaaa gtcccctgat ctatgctgcc
     1501 gaggaacagg gcgtgcccat cggttatgtg atcgatcacc cttatgatga gaaaagcagg
     1561 gagatcggct gggtgctgct gcccgaatat tgggggcatg gctatgcgtc tgccctgact
     1621 gatctgctga ttcaaagagc cagacaggaa cgcaggcagc tggtgatcga atgcgctccc
     1681 cagcagcagg caacgaaacg cattgcggta cggaaggggt ttcgttatca gggctt
//
LOCUS       DAWAEA010000305         1717 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_8945_length_1717_cov_1.604693, whole genome shotgun sequence.
ACCESSION   DAWAEA010000305 DAWAEA010000000
VERSION     DAWAEA010000305.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8945_length_1717_cov_1.604693"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          361 a          518 c          507 g          331 t
ORIGIN      
        1 gcacctgctg agcgcctccc agaccggcta ttctgggcag attttttacg atgggcagga
       61 gctccgagac atccgccgtt cctctctgta tgacctgatc tccgtcattc agcaaaacgt
      121 gttcatcttt aacgccacca tccgggaaaa catcaccctg tttgcggatt tcccgaaaga
      181 agcggtagag cgggccattc gcctgtccgg cctttccggg ctgattcggg aacgcgggga
      241 ggactatctc tgcggggaga acggaagcgg cctgtccggc ggcgaaaagc agcgcatctc
      301 catcgcccgc agccttctga aaaacgcgca ggtgctgctg gtggacgaag ccaccgccgc
      361 attggacgcg cagacggctc atcaggtctc ccatgccatt ctggacttaa gcggcatgac
      421 ccgcatcgtc gtcacgcaca cgctggaaga aagcgcgctg cgccgctacg acgcgatcct
      481 gaccctgcgt gacggctcca tcgcggaaca gggttctttt gaagaattga tggaacgccg
      541 ggggtatttc tattcgctgt ataccgtttc ccaataaagc ctctcgaaaa cccaaccggg
      601 attcaacctt tctcgcccgc cgggtcattg cgcccggcgg gttttgccgc tattctgaaa
      661 gccgttcagg gagcccgggc ccggacagaa catccatcct gtatgacgga ggtttcctta
      721 tgagtaccct tgatttcggc ggaaggatca agacccttcg cctttccaaa agcatgacgc
      781 aggaacagct ggcccagcgg cttcgggtca gcccgcaggc cgtatccaaa tgggaaaacg
      841 gcgtgaccct gccggatatt cagctgctgc ccgagctttc cattctgctg ggcacctcga
      901 ttgacgcgct gttttccatg acggacgaca agcggatgga gcgcatcgac aacatgctct
      961 gggatcagcg ctttctgtcc cgggaggaat ttgagaccga ggagcgtttt ctgaaggaca
     1021 aatgtctgga gccggacgcg caggcccggg ccacgcttct tctggcggag ctatactgca
     1081 agcgcgcccg ggaataccgg gagctggcct ccccgctggc ccgcaaggcc atcggtctgc
     1141 tgcccgaata tgacaaggac gcccacacgg cgctgttcga ggcggaaggc ggcgtcttcc
     1201 cggactggaa tgacgccaat catcaccgaa ccatcgagtt ttacaaggat ttcatcgccc
     1261 gtcacccgtc ccagcgcagg gcgtacttct ggctgctgga tctgctcatt gacgacggcc
     1321 gctgcgagga agcgcgggct tatctggcca aggccgaccg gctggaaaca agctatcgtt
     1381 ctgatctgta tctgggcatg attctggaaa aagaaggcaa gccggaggaa gccgccgcct
     1441 gctgggagcg catgacgcaa aaggatccgg acaactggca gagctggttc gcccgggccg
     1501 accggctggc aaagcgctgc gcctatccgg aagccatcgc cctgtatgaa aaggcgctgg
     1561 cgctttcgtc ccggccccgc tttaccgacc cggtcaacgc catcgcccag ctgtatgaaa
     1621 ttcaggggca atacgaaaag gcgatcgaat tttatcagaa ggagatcgag atcgtccagg
     1681 aggactggaa cgagactcag ggcgaggcca tcgacga
//
LOCUS       DAWAEA010000306         1712 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_8964_length_1712_cov_1.662643, whole genome shotgun sequence.
ACCESSION   DAWAEA010000306 DAWAEA010000000
VERSION     DAWAEA010000306.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8964_length_1712_cov_1.662643"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    388..397
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1286..1355
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          356 a          485 c          503 g          288 t
ORIGIN      
        1 gtggaaaaga cgcgtgaaga atcctacttc ttccgcctgc agaagtacgc cgaatggctg
       61 attcagtaca tcgaggagca tccggatttc atccagcccg cctcccgcaa aaatgaaatg
      121 atcaacaatt tcctcaagcc cggcctgcag gatctgtgcg tcagccgtac ctccatccag
      181 tggggcatcc cggtggactt cgaccccaag cataccgtgt acgtatggct ggacgcgctg
      241 accaactaca tcaccgcgct gggctacact accgatcacg acgagctgta ccggaagttc
      301 tggcctgccg acctgcatct ggtgggcaag gagatcattc gcttccacac catcatctgg
      361 cccatcatcc tgcacgcgct gggattgnnn nnnnnnnggt ggaccccgtg gtgctgtgca
      421 acggctacgg cagcgacgcc atccgctact tcctcatgag agagatgccc ttcggcagcg
      481 acggccagtt cagctacgaa gcgctgctga accgcatcaa cgccgatctg gccaacgatc
      541 tgggcaatct ggtcagccgc accgtggcga tggtagagaa atactttgac ggcgaaattc
      601 cccagcacgg ccagtggaac gacgtagaca aggccctgat cgcgctggcc gagggcacgc
      661 ccgacaaggc ccggcagcat atggacgagc tgcagttctc gctggcgctg caggacatct
      721 ggcagctggt gggcgagtgc aaccgctata tcgatgtgaa cgcgccctgg gtgctggtaa
      781 agaccaacga gggcaaggaa cggctgaaga ccgtcatgta tgtgctgtgc gagtgcattc
      841 gcatcgtggc gatcctgatc gcgcccacca tgccccgtac tcctgcccgc atctacgagc
      901 agctgggcat tgccgagggc gagctgaccg gctgggagtc cgcttcccgc ttcggcctgc
      961 tgcctgcggg caccaaggtg cagaagggcg aggctttgtt cccccgtatc gacgtgaaga
     1021 aggaactggc agacatcgtg cacaccccca aggcggaagc gcctaaagcc gaggaaaaga
     1081 agcccgccaa gaaggaagaa aagaaagagg agaaactgcc cgagggcgtg atctccattg
     1141 acgatttcgc caaggtgcag cttcgggtgg cccgcattct gaccgccgaa aaggtggagg
     1201 gcagcgacaa gctgctgaaa ctgagcgtga agctgggcga aggtgagccc ctgcgcaccg
     1261 tggtcagcgg cattgccagg ttctannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccatc tagggccgtc aggtggtgct
     1381 ggtcagcaac ctgaagcccg ccaagctcaa gggcatcaag agcgaaggca tgatcctgtg
     1441 cgcctccgat gcggaggaca aggtgctcaa gctctgcact gtggaacccg gcatggagga
     1501 cggcgcgtat atccgctgag ccgccgaacg atatgatgaa tttgtttgat tcccattgcc
     1561 atctggaaga cgagcgcttt cagggcgaag tggaacaggt gctttcccgc atggcagcgg
     1621 cgggcgtgaa ccgctgcatc tgtgcgggaa gcgatttgga ttccagccgc cgtatcgcgg
     1681 cgctgaccgg cgagcacgcc cccatctacg gc
//
LOCUS       DAWAEA010000307         1704 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_8988_length_1704_cov_1.910249, whole genome shotgun sequence.
ACCESSION   DAWAEA010000307 DAWAEA010000000
VERSION     DAWAEA010000307.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8988_length_1704_cov_1.910249"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          308 a          537 c          476 g          383 t
ORIGIN      
        1 tggttttgtt ctcgaagata aagttgttca tcgtccatgc ttcctttctt tattcacaga
       61 tccagcgggc gatattctgc gcccgttcca ggtacgccgg gtttttccgg caggtatcgg
      121 cattgccgca atggggaaag acctctgtgc gctccgcctc caccgagcgc tgtatgctgg
      181 gcagatcgac gatcaactgc acgtgatgct tcatttcatc cacgtccgaa ccggtgcagg
      241 tgagcagcac gccgatcttc ttcttcggcc ccagctgata atttttgcag gcgaacgtgc
      301 agcacaggcg attgatcacc agcagcgcct gtgccgccat gcagttgtag taaatgggcg
      361 cttcggcaat cagaccgtcg cacgcatgca gcgcgcttaa aacggcgcgg aaatcgtcct
      421 tctgcacgca ttcgccgatc ctggcgcagc cgtagcagcc ccggcagggg tggatgtcca
      481 tttcgcgcac gttcaggcgc ttcacctccg tgccgcagcc catcgccgtt tctgcggtgg
      541 cttcgatcag cgcgtcgccg ttcccgccca ccgtgggcgt ggttgtcagc agcaatacct
      601 ttttcatttc gcctgccctc catgattcat ctgtttttcc cagaaggccg ccgcgtcgtt
      661 cagccagccc tccgccactg tgccggtgcc cagcccgaag ccgtgtccca gtcccgcata
      721 gacgtgaaac tccgtgggtg tgcccagctc gctgagcagc tccagccgcc gctgcatcag
      781 ccgccagctg gcaatgccgt cgctgtcgcc cacgcaggca taggtgggcg ggtcaagctc
      841 gctgtattcg ctgtggccgg tgtattgcag gatgacggcg gcaggccgcg gtagatcggt
      901 ttcgccaaac gccgccgtgc cgtaggagcc gacccacgct gccatgcgtc cgcctgccga
      961 accgccccag agggaataat tgatggtatc cacctccagc atttcagcgt tttcaaagat
     1021 gaagcggatc gcccgcgcca gatcctcgca cgccgtctgc gcgccggggc ggtaaatcag
     1081 cgcaaaggcg ttatagccct tcttcgaaag ctccaatgcg tgcgggaaac tgtcgtgcat
     1141 cgcgcccaca taggcgaatc cgccgcccgc attgcacacg gcgaatttcg cgccgggcgt
     1201 acccttgaag aagaataggc ccgtgtcgcg cttgtcggga tcggcggcct tctccgcctc
     1261 ggtgtaaatg tcgtaaaaga tgatttcgcc gttcaggacg cgattgcgca gggtgttcac
     1321 gatctccacg gttttgtccg gatcaatgtg gctgtaccac gtcaggcgca gattgcccag
     1381 cgtatcaccg ctgaaatagc ccgcgtccac cggaaagagc agccgcccgt aatcgccgaa
     1441 ggcgggatcg tcgatcaccg tctgaatggg cgtatttcga tcaaacattt ccgtctgcgc
     1501 tccttccgca tgagcggctc cggctgtcag tgccagcagg cagattgccg tcagcacccg
     1561 gcaaagccgc ccgaaggttc ccgcttgtct catgctttca ccccttcttt tggtttcgcc
     1621 tttattataa gaaaagcgag gcttcaggag aagtctcgct ttgttcatga gtatatacct
     1681 ttgggttata ttaaccgtac agtt
//
LOCUS       DAWAEA010000308         1701 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_9007_length_1701_cov_1.629405, whole genome shotgun sequence.
ACCESSION   DAWAEA010000308 DAWAEA010000000
VERSION     DAWAEA010000308.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9007_length_1701_cov_1.629405"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1188..1197
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          463 a          415 c          484 g          329 t
ORIGIN      
        1 gtcgtacgac cggccgcctt ttttgcgtcc ttttgacagg gagggtgaat gaaaacgaac
       61 gacaaaaaag ggtttttcgg gcgcgagctt cgatgaaaaa ccgcgttatg aacgaaaaac
      121 gacgcgaatt tggggtggag agataaaaat agcgagcggc gaactgcggg gggaaattcg
      181 cggttcgctg ctgttttgca atagatgctt gatgcaaaac aaactgtgcg ggagatccaa
      241 tggcgatgaa ggataaaaaa ataggaaaaa gattttgaat tgcgaaatat aaaatcttct
      301 cttacagttt agagaaaaaa caacagcctg aaaaaagatg ggcatattct gccgttttaa
      361 ggggaaaaaa gagggaaaag gagggggata acatgacata tccatatttg ttaaggaaga
      421 aaaacgtttt gataacgttg acaaagggca gaaggaggag taaaatacag acgtttgatt
      481 tgaacagaaa ccaaacggat tgacgcaaaa gatttcggcg atgcaaaaag gaggaaaggc
      541 gcatgagccg caattttgga aaggtctgga actgcgtaag caccgtcctg gtggcgctcg
      601 tcgtgctgct ggcggcctta cttgccggag ccaggctttt tggtttgcag gttttcacca
      661 tcctgtccgg ctcgatggag cccaagtacc cagtcgggtc gctgatttac gtaaaatccg
      721 tcgatccttc gcaggtgaag gagggggacg tgatcacctt cctgctctcc gataccgtca
      781 ctgcgaccca ccgcgtggtg gggatcgtgg atcagggcga cggcgcgctc cgcttccgca
      841 ccaagggcga cgccaacgac gcggaggacg cggcgctcgt ccaccagaac aacctgctgg
      901 gcgaaccggt cgcctgcatt ccgtacctcg gctattttgc cgcctgggtg cagaacccgc
      961 cggggagcct catcgccatc ggcgcgggcg tgttcctgct ggcgctggtg ttcctgcccg
     1021 atttgctggg cggaaacaag gagaaaaaga aaacagatgc ctccggggat gaaaaagaca
     1081 catccccgaa cacataattt ttgaaaaaca ggaggaagtc cccatgaaga acaaaacgaa
     1141 ccgtaccgtg tcgatcaaga tcgctgcgtt gatcgccgcc atcgtgcnnn nnnnnnnata
     1201 tcaacgcgac gcttaccgaa accaagacgg cattcaagat cgtccccggc gtggacatcg
     1261 acaaggaccc cgtcgccacc gtcgaggcca atagcgagga ttgctacctc ttcatgcagc
     1321 cgactgagga aaattggccc aaattcaaga ccaccgaggc gggcagcacc acccgcaagg
     1381 tggaatacga gattgcggac ggctggatcg agctgacgcc gaagcacggc gtttattacc
     1441 gcgaggtcac caagagcgac aaggatcagg tcttccacgt gctcaagaat gacaaggtga
     1501 ccgtgagcag caccctgacc aaggaagaag ccgacgccat caccggcacc cccaagctga
     1561 ccgtggccgt ttacgccgtg cagaaggaga acatgggttc tgccgccgag gcttgggaga
     1621 aggcaaatgc ccaataattc gtctttattt ttcggtgctt ctgtttttta cacaatcttg
     1681 aatggaaagg aagtcccagc c
//
LOCUS       DAWAEA010000309         1690 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_9073_length_1690_cov_2.032416, whole genome shotgun sequence.
ACCESSION   DAWAEA010000309 DAWAEA010000000
VERSION     DAWAEA010000309.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9073_length_1690_cov_2.032416"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          345 a          476 c          578 g          291 t
ORIGIN      
        1 atgcgaaact gcgacaaaag aaatggagtg ttgaatcgaa tggcgcgttc attggccaga
       61 cgggctgcca tgcagcttct gtacgaacgc ctgtcaggcg gcgaggcgga tcaggaatcc
      121 ctggagatgg tatacgagca gttatccgaa gagggggaaa agcctcagcg cgtgaatccg
      181 ggcccggagg atcgggccta tattgacgat gtgctgcagg gtgttcagac ctatcagcag
      241 gagctggacg accagatcgc cagccattct accggcgaat ggaccatcag ccgggtaccc
      301 catgtggatc tgagcattct ccgccttgcg gcctacgaga tgctccaccg cagcgacgtt
      361 cccgacaatg tggccatcag cgaggcgatg gagctggcct cccggtacag cgagccccgc
      421 agcagccgct acatcaacgg cgtgctgggc gccatggaac gcgacaagca ggaaggctga
      481 tctccatgga cgcagtgttg ggtctggata ccagctgtta taccacttcc gccgccgcgg
      541 tggacgcgca gggaaaggtg atcgcctttt cccgcctgct gctccccgtg gaggggggcc
      601 agcgcggcct tcgccaaagc gaggccgttt ttgcgcatgt aaggcagctg ccgcaggtga
      661 tggaaagcct gatgacagaa ttgccgccgg aggcgacgat tcgcgccgtc tgcgcgtcct
      721 ccaagcccag ggacgaggcg gacagctata tgcccgtgtt tcagaccggc tgcggctacg
      781 gacgggggat cgccgccaca ctgcgggttc cgtttttcga aacgacccat cagcaggggc
      841 atatcgccgc aggatgcatc ggccttccgc cgctggacga gcgctttctg gcggcacagc
      901 tatgggaaaa gcaggtctgt accccggagc ggatcgccgc cgagaccttc gatctgctgg
      961 cccggacggt cagccggatg ctggcagcgg gatgcaagtg ctatcaggtg cgtcaggcgc
     1021 tggtggtagg cggcgtagcg tcttcaaagc tgctgcgcgg acttttgcag cagcggctga
     1081 ataagctccg ttcgccggtg gaggttttct tcggagcccc ccgctacagt gcggataacg
     1141 ccgccggggt agcggagctt gggctggaac aatacaggca gtggaaggaa caccacgcct
     1201 gaaacaggcg cttgattcga aaacagggag ggacgggcaa tggcggcgaa gattctggac
     1261 ggcgcggcaa tggcgctgga ggtcgaaaaa gaactgaaac agcgggtgga agccctgcgg
     1321 gagcgcggcg ttgtgcccgg cctgtgcgtc attctggtgg gcagcgatcc tgccagccag
     1381 acctatgtag ccaacaagga aaaagcctgc gctcggctgg gaatccattc ccacaccgtc
     1441 cgcatggggg aggagactac tcaggcggag ctggaagctg agatcgaaaa ggccaatcat
     1501 gacgaaagca ttcacggcat tctggtgcag ctgcccctgc ccgctgggct ggacgagcac
     1561 cgggcactgg cgctcatccg cccgggaaag gacgtggacg gtttccatgc ggtgaacatg
     1621 ggtcgtctgg ctcggggaga ggattgcgtg gtggcctgta cgcccaaggg agcgctgcat
     1681 atgctcaagg
//
LOCUS       DAWAEA010000310         1687 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_9087_length_1687_cov_1.951593, whole genome shotgun sequence.
ACCESSION   DAWAEA010000310 DAWAEA010000000
VERSION     DAWAEA010000310.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9087_length_1687_cov_1.951593"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    425..453
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1373..1414
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          345 a          464 c          436 g          371 t
ORIGIN      
        1 aaaaccatcg tctcgtcccg ccctgtcgtg cgctctacct gttggtgagc cataactggc
       61 ggcaaaatag acccatgacg atccctttct tcgtgacagt ccagcggtgt agccgtcaaa
      121 gcatccccac tgctggaagg aatgccccct gcactcattt ccgaacgttt tgtcctatct
      181 gtctctgctt tgtactcatt tcgcttcttg acacgatcga tattcggtat tacaatgtta
      241 aaggttcgaa aaaatccgta cattttgcaa gactgaaagg agaaacgttc atgaaaaagg
      301 ttctgtctct tgtctgcgtt cttctgctct gcgccgcttt gatccccgcc gccgtggctg
      361 aagaagccgc ctataccgag acggtggtgt ccatccccaa tggcgaccat gatattcccg
      421 cgacnnnnnn nnnnnnnnnn nnnnnnnnnn nnntctgcgt tcccaacggc gaaggcaagt
      481 tccctgctgt ggtgatgctc cacggcaccg gctccaaccg cgacgaagcg ggcaacggct
      541 ataaaatggc tgctcctgtg ctggctgaaa agtatggcat cgcgaccgtt cggatcgact
      601 tcatgggcag cggtgattct accgccgact acaccggtta caccttcgaa agcgctgtga
      661 gcgacgccgc cgcggctgcc gcctatatgc agacgctgga aaacatcgac gctgataaga
      721 tcggcgtgat gggctggagc cagggcggca ctgacgcgct gctggccgct gcccgtcatc
      781 ccgagctgtt caagtctctg gtgacctggg ccggcgctcc cgacctgagc gatatgctga
      841 ccgatgagct gtatgaagaa gcgaagaaga acggcttctt cgtcatgacc ttcgactggc
      901 gcgattctct gaacgtgtcc ctgcagtggt gcgacgatgt gaccaacacc gacgtgctgg
      961 ctgagttcgc cgccggttac accggccccg tgctggccat ccatggtaag gaagacacca
     1021 ccgtggaccc cgagtggagc aacaagatcg tggccgccag caaaaacgag gcttccaaga
     1081 cctacttcat cgagggcatg gatcacacct tcaacgtgtt ctccgaggag gacttccact
     1141 ccctgtacaa tgcggtggac gctaccggcg agttctttaa ggccacgctg gcggactgaa
     1201 gctgaccccc tgccagagcg tttgctttgg aaaatcggaa aaactgctgc aaagctctcg
     1261 ttttgcagca gtttttttgt gcacgtttgc gcgctattcg ttttttcagc agccgtatta
     1321 tgcgcaaaga cagctcgtca cccgatagac cggctggcgc cgtccatgaa acnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnattgcc cgtcagggtg attcttgggg
     1441 tgctcgccgc gccgtcgaag gaaaaatgga tattgtgata ggaaccgtta ttgatgcggt
     1501 aacaaaagaa ttccgccatt tcttttctgt ttcttccggg acagtccaga acgacgcaga
     1561 tctcgtcatc gtttttctgg tcgtccagca gaaagtcata tacgacggcg tgatgcttgg
     1621 ccagaatact gggacgcacc gccgggtgac ggacaaagcc ctccacctgt tccggggtga
     1681 aatgcca
//
LOCUS       DAWAEA010000311         1684 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_9108_length_1684_cov_1.799877, whole genome shotgun sequence.
ACCESSION   DAWAEA010000311 DAWAEA010000000
VERSION     DAWAEA010000311.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9108_length_1684_cov_1.799877"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1025..1110
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          406 a          362 c          441 g          389 t
ORIGIN      
        1 gcatggaaca ggagcggggg tatttcgttc atcccagcag ctatgtggat gaagatgtga
       61 ttgttggcaa gggaacgaag gtatggcatt tctgccatct gcaatccggc tgccgtatcg
      121 gcgaaaactg ctcgctgggg cagaatgtga atatctccaa caatgtgatc gtgggcgacg
      181 gctgcaagct gcagaacaac gtttcgcttt acgagggcgt gattctggag gatcatgttt
      241 tctgcggccc gtcctgcgtt ttcaccaacg acctgacccc tcgggccagg tatcccaagg
      301 gacgagccgc ctataaacag acgcgtatct gcgaaggtgc gtccatcggg gccaacgcca
      361 ccatcgtgtg cggccacacc gtgggggcgt gggccatgat cggcgcgggg gccgtcgtga
      421 ccacggacgt gccgcctcac gcgctgatgc tgggcgttcc ggcccggcag gtggactggg
      481 tgtgcgaatg cggcgcacgt ctgcacggga agaaaatctg cccggaatgc ggccgtgaat
      541 gggataacga cgcgcttaaa gacgaaaaat aagaaatttt ttctacaaat aaaatatgct
      601 tacgattata aaacaatgag agtaagcaca ttgcttcatt cgtatcgttg aaaaaacttc
      661 gagaatgtcg gaaacttttg attggctgcc actcctgtga aaaaagagat aagggacaaa
      721 ttttgaccat cagtcagata ttctcattta tactccattt gatggggaat gtaatgcttc
      781 aaattctctt ggccatcata aaggtacgtt gacatagcca aacgaggggt gtataaaaaa
      841 tagagatagt tcttaaatcg aaagaaggtt cttcacatgg gtttgttgat ccttgtcatt
      901 gtgttcgttg gattgtttgt atttctgttc gttgctgctt tcaaacaggc gaagaaagaa
      961 gaacaggagt gggaagaaaa gaaagtcgct catagagcgt ggctgaaaaa gtttggctac
     1021 gacannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctatcgtttc gtgcgagctt attacaaaag
     1141 taagctctac cacaactacc gatggcggtt tagctgcggc tgcaatcggt gggcttgtcg
     1201 gcggcagctc tggtgctgtt gttggttcca atatggctcc gcgaaccacc acttttaata
     1261 ccgatgtaga gggtgttaaa atctatttta acgatgccaa caatccctcc atggtattaa
     1321 aattgaaaaa agaagcgtct atcgccttat atgacgcact ttatgttatt ttggctcaaa
     1381 aataaataca gccccgaggc tcaagagctc cggggctgca ttttactgga cttctttcaa
     1441 aattgcatcg acaacctgat cctgttcttc gcgggtaata tagggagaaa gtggcagggc
     1501 cagcacccgg ctgcaaagat cggcgctgac ggcacagtcg cagacctgct gatcgtggat
     1561 ggatgcaaag gccgtctgct ggtgcatccc gcggggatag tagatcatgg cggggatctc
     1621 ctgccccttc agaacggcca tgacccggcg gcgttcatcg gcgtctcgca gcaggatgga
     1681 atac
//
LOCUS       DAWAEA010000312         1684 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_9106_length_1684_cov_2.011664, whole genome shotgun sequence.
ACCESSION   DAWAEA010000312 DAWAEA010000000
VERSION     DAWAEA010000312.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9106_length_1684_cov_2.011664"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1171..1180
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          310 a          569 c          460 g          335 t
ORIGIN      
        1 tccagccccc aaaggcggcg gagacgctgc cttccctccg ttcccagctt ttccaagcag
       61 gcaaacagtt cctccggcgt atagcgaaag cgcatatccg gcatttcgaa ctggctgtcc
      121 acggccttca aagcgggata gccccgcttc ttatccccgt aaataccgta aaggcaggct
      181 gcggaaccca gcgcgcctac gatggtcaaa atcagccacg ccgtgctgct cattcctttt
      241 ccccctgccg cagggtcagc cgggtgcagg gcacgcgacc agacagaagg tcatccagac
      301 ggcactgggc gtggccgatc atcacattgg tgccgtgctc cactgcccgc agggcaaagg
      361 ccagcttggc ctttgcgccg ttggcgcccg cccggctggc tccctcgcag tggcccatca
      421 gctcggccac cttctgccgc atttcttcaa tattgtccgc ttccacctgc tccaccagcg
      481 tggaggggtc gtccttgttc aggtaaatgc catgggtgcc cgtcagcagg atcagatttt
      541 tggcgtgaac cagccgcgcc accacggcgg cggtctcgtc gttatccacg cattccacca
      601 cttccatgcc ctcggcccgg cggctggcca gctccagctt gctgacttcc tcgtcgttca
      661 ccgggtcgtt gtagttgatg atgggaatgg cgttctgctc cgccgcccgg tacagcatct
      721 gccggatgtg ctcccgccgc tggggatcgt tgaaatgggt gtgctccacc aacacctgcc
      781 gcacgccgta ctccggacgg atgaaggtac ggtaattgtt catcaggatg gactggccct
      841 gtgccgaata gtccaccttg atctgctcct tttcaccggt cagctcgtgg ccggtgcgct
      901 gaatgtagtc cagacggccg atctccgtcg cgccgctgga gaccaggatc atgccgggcc
      961 gcagcgccat ggccaggcgt tgaaatacat tatagtcaat gacgctgtct tccttctgga
     1021 tcagcgccat actgccgatc ttcacgacca gatcaatgtt ttgcatctta cgcgttctcc
     1081 cttgcttctg tcagaacatg catccatttg cggctcttga gtcggggaat gccgattgcc
     1141 attttgacga cttcctccac gatggtgcac nnnnnnnnnn ggtaaacggc ctccagcggc
     1201 cagtgccagc agagggcagc cagcccggtc aggggcacgc cgacgatcca caaagtcccc
     1261 gcctccagag caaggctgaa caccgtgtcg ccgccgctgc gcagcacgcc taccacgttg
     1321 accgtattca gcgcccggaa ccagatcgca gcgccgccga tggtcaaaat cagctgggcc
     1381 ttctgccgca cactttcgct caggccggag aacaggttca ccatgggcca gcgcagcaga
     1441 tagaccgcca cgcccagcag aagccccgcc agcacagccc cggagatcag ccgtttgccg
     1501 tacagatagg cccgttcctt atcgccgctg cccagcgtct tgcccaccag aatggcacag
     1561 gcgttcatca gggcgaaaat cgccacccaa accagatcgt tgatggtgtt gcacacgccc
     1621 atggtggcta ccgccgcgtc gcccatgcgg ccatagaata cgctgaacat ggtggtgccc
     1681 agtc
//
LOCUS       DAWAEA010000313         1679 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_9139_length_1679_cov_1.801724, whole genome shotgun sequence.
ACCESSION   DAWAEA010000313 DAWAEA010000000
VERSION     DAWAEA010000313.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9139_length_1679_cov_1.801724"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          356 a          455 c          511 g          357 t
ORIGIN      
        1 ggcatgaagc atttcgtgcg ctttgggttg tacgagatca aaggctccat caatgtccaa
       61 ttggcggaaa agacgggttt ttctcaggaa gacgcgcagg tcgtgaagga gtgcctacgg
      121 acgctgtttg taaacgatgc ttcttcagcg cgtcccgaag gctcgatgga ggtcgtgaaa
      181 ctttattggt gggagcataa ttgcaaggac ggccagtatt cttctgcaaa ggtgcaccgc
      241 agtgttcatg tgactgcgaa ggatattgcc gcgcttccga atagtgtgga ggactatacg
      301 atcacgcttg acgaactgcc cggtctgatg ccagagatca ttgagggagt gtaagcggaa
      361 acggcctgaa aaaccaatcc gtttcgcact gcgggggaat gggaaaaagg gatagaccct
      421 ttccgtcctg atggtgcgct tcttcctact gaaaacaccg ctgccctacg gcttttacga
      481 ccggggcagc ggtgtttgat tcataagaaa tggaaaattc ctcggcctta cgcctgaaag
      541 aacgccgtgc agagccggac gaaagcgtcc tgatctgccg cacccatggt ctcgcaggcg
      601 gagtgcatgg ccagaatcgg aacgccgatg tctgcggcag gcatggccag cagggacgag
      661 gccatggagc ctaccgtgct gccgccgggc aggtcggccc ggttcatgta aatttgcagg
      721 ggaatctggt tggcgtcgca aagcccctgt atgaccgcgc aggtctccgc atcggaggaa
      781 taacgggggc tgcgcttgag cgcaacgccg ccgcccagca ccggcgcaca ggaaaaatca
      841 gcgtattcgg gatgattggg atgcatggcg tgggccgcgt cgcaggaaag cagcatcccg
      901 ttcagacagg cgtccagcag cgccgtgcga tccagcccca gcgacagggc cagtttttcc
      961 agaatcaccg tcagcgtgct gctgtccgcg ccccggcggg tgttgctgcc gatttcctcg
     1021 ttgtcgtaga gaacagccac gttgataccg tcgccctgcg catccgccag ccacgtgttc
     1081 aggatcgcac cgccgtaggg ctcaacgctc agacggcagc agccgccgga gatcagttcg
     1141 ggctggggct tgatacgcat acagggccag tctgtgtggg ccgccgcaat gcggatcacg
     1201 tctttctgcc ctacggtcac ggctgccaga aaggtgccgt tttccacaaa atagcgtccg
     1261 ccccggtgaa cggagaaggg cttgttcagc gcaaggggtt caaagccccg ctccgtcagc
     1321 cagtgggccg ccatatctac ggtatgccac ggcgaatgac cgccgccgag ccatttcatc
     1381 agttcattgg taaattcagc catgttctta gctcctttgg tgggttctgc gtgcgggcaa
     1441 accgctgccc cgcgccgcgt tcaagggtat tatatttctt tttggtcaaa gaaacaagtc
     1501 ggaatcccac agccgaaaac gtggaaaatg tggaaaaaac cgcttgctta tggtacaatc
     1561 gggttgtcct gttcaggacg ttacaggagg tcttggtgtt tcagatattg ttcgtctgcc
     1621 atggcaatat ttgccgcagc cccatggcgg aatttgtcct gcgggatatg atcgcccgg
//
LOCUS       DAWAEA010000314         1677 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_9154_length_1677_cov_1.629470, whole genome shotgun sequence.
ACCESSION   DAWAEA010000314 DAWAEA010000000
VERSION     DAWAEA010000314.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9154_length_1677_cov_1.629470"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    252..287
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    756..765
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1308..1430
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          333 a          405 c          397 g          373 t
ORIGIN      
        1 gcggtagccc agcatttcct tcgggcagct gcgcagaatc agggactcct tcaaccgatt
       61 ggagtccaag tcataggtca catctgtgtc gtgataaaca ttctgataca cggcctcact
      121 gcgctgattc ttcagcgcag cctctggctg aagctggtca tcgtctgcca aagtggaagt
      181 ggtactgctg attgccgcag tactttcgcc tgcgggaacg cagatcatat cctcaccagg
      241 cagttcctcc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa ggataggcta
      301 tatccattcc ggctgacagt gatcccgccg gcggtgttca gttccgcggg taccgagaca
      361 tcccacatac cggcggcatt ctgataaacc gtctcgccgt cccgcgtagc cacaggcaga
      421 aggcgattat cgatatcctc ccactggcca tctttttgat aatgaacggc tgcaggatag
      481 ataactacct gccgcacgcc gttggccagc agaaattctt tctggttttc gccccgtcgc
      541 tccaggactt ctcctattac atcgggaggt gtctctgcga tgttttccgt tgtcagcggc
      601 cacaggtccc ccaactcttc agccatgacc tgcacgggga gcaggtatac ggtcagaagg
      661 agcgccagga aacaggacag tatgcgtttc cacagtttca ttcgtttttc tctctttctc
      721 tttgtattca ggcgctggtt tgtgcgtcta tttatnnnnn nnnnngtccc acttcctatt
      781 tcgcaacaaa attcgacata ttttgcaaaa tttggaacaa ttaccaatat gccgcattga
      841 aattgaacct tttgactaag aaaattgtcc gtatgttccg gaagcatatg acgaatcgat
      901 ccggaaaata tctggtgttc tgcgccaata aggagcatat ggatgagctg ctctgtcatg
      961 tgtcccagtg attcggcgga gtagacccgg agccccatgt ttaccgggcc tattcggagg
     1021 acccggaaac cagccaggcc tttgctgctt tcaaggcgga cggcagtgcg catttgaagc
     1081 tgcttttttg tattgacatg ctgaacgaag gcatccacac gccggatgtt tctggagtta
     1141 tcctgtttcg ccctacagtt tcacccaccg tctacaagca gcagataggc cgggccctgt
     1201 cctgcggctc agccagaact ccgctgatac tggacgttgt caacaatgtg gagaatttat
     1261 atagtatcgg aaccatccag gaggagatgc aggcggctat cacctacnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn attttaccgg
     1441 gagcacggac acctgaatgt gccgaagcac tataagacgc cggagggtta ttctctgggg
     1501 gcctggctgc aaacccagcg ccgtgtgcac tgtgggtccg ttccgggtac gctgacagag
     1561 gaacaggccc gccggctgga tggcatcggg atggtatggg acgtccagca gctgtcgtat
     1621 ttgacggaag aacgcatccg gcagctggat gaactgggct ttatctggag caaggta
//
LOCUS       DAWAEA010000315         1674 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_9172_length_1674_cov_1.545398, whole genome shotgun sequence.
ACCESSION   DAWAEA010000315 DAWAEA010000000
VERSION     DAWAEA010000315.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9172_length_1674_cov_1.545398"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          340 a          459 c          528 g          347 t
ORIGIN      
        1 gttcagcacg gggccatcgt cgtaggtcag ggcgatgatg gggagggcgc tgttgtcggt
       61 ctgacggtcg gcttcggcga tcataaattc ccggtagtcc cggtacagca gcggaacctg
      121 taccagctgc catttgccgt ccaccgcgtc ctccagggga aaggtcagca gaaaacgccc
      181 ggcgcaggga aggaaaggca ggtcggccag cgaagccgta ttggccagct ggcggatcgc
      241 cgcaccatca tgagatgtat caggatagta gagggcaaag cgttcctcga ttgcccggct
      301 gatcatctgc cagaccatgc tcccctcggg aaaaacgtcc cgcaggctta gcggcccgcc
      361 gtccagcaga tcgtagctga aaacccggct ggtcaggtag acggtctcct cagtgtcgaa
      421 ggtatcgttt cggctgcgcc ccgtgcgaat ggcgcggccc gtcagaagga agcccgccca
      481 gctttctccg ctgatgcgga aggtagagga aagctccagc acttcatccg gggaggaatg
      541 ggcctgaagc tcctgccaga tttcctccag ggcggcccgg agcgcctgat taacgctctc
      601 ctgacaggtg acgggcaggt cggcctcggc ctgccgtccg tccgcagccg tccaggactc
      661 ggtctccatg cggatggtca gttcctcggg aaaggggatc accacgtcgt gaccagcgag
      721 cgcgggaacg caggggagca gaaaaacgag cgccgcgacc catgcggcgg ctctgcgaag
      781 cagcgttgct ttcatgccgt ccctcctttt ctgacaggaa ggatagcata tccgaccggc
      841 ggtttcaagc ggagcggggg cgaaaacgca aggatttata ccttgcacac cgccggtttt
      901 cggagtacaa tataattgag gaaaaatctt gaaattctgt attgacgggt tatctcattg
      961 gggatataat gtctatgttg atatgagtgg tggagtctgt caccgaggcg aaaaacctcg
     1021 gttcatatcg aactgtgaga cattgtcccg caaaccgaac attatttcca ccgcccagca
     1081 tcgttcatcc gacgattggt gacggtgttt tttgtgggca aaattgcgca gggaaggagc
     1141 ggcagccgcg gagacacgtg cccgagaggg cgtatgcgat gcctttgaaa gatcctcagc
     1201 ttttttcgag tgaacgcgat gcgaacgcat ggtattcatc cctgattcta ttttataaag
     1261 gaaagttgag gatgctctca tggctgcttg gttgatcgtt ctgggcgtga tcgtgcttgc
     1321 gattgcctat gtgttctaca attattttcg gattcgccgg atgggcgaag gaaccgccga
     1381 aatgatggaa atggcggaaa ttatccgcag cggcgccgtg tgcgtgctgg gaatgcgcag
     1441 cgctacctat gccaacgtgc gcaccgccaa ccgcgcccgg gagactctgt ccatcggcga
     1501 gaccgtgaag gtggcgctgt gcggcggcag catttccggc ctgagtgtgc aggccttcgg
     1561 aatgctgggt ctggtgctgg tgctggtcat ctggaagggc atcgaccctg ccgccacggg
     1621 ccggggtctg ctggccaacc tgccctgcac cccctccatc atgcgggtca cgac
//
LOCUS       DAWAEA010000316         1673 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_9175_length_1673_cov_2.344870, whole genome shotgun sequence.
ACCESSION   DAWAEA010000316 DAWAEA010000000
VERSION     DAWAEA010000316.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9175_length_1673_cov_2.344870"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    519..528
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          356 a          499 c          460 g          348 t
ORIGIN      
        1 ggcgtgcttg aagatcacca gcgagtcctt cacgcccttg gcccgggccg tgcggatgta
       61 atcctgcccc agcacgtcca gcatactgga cttggtcagc cgggtgatgt aggccattgg
      121 gtacatggcc agcgacagca ccggcagcac gtagttggga ttggaggtgc tccatacgcc
      181 cacccactta agctgcaggc agaacaccag cagcagaatg gtggccagca cgaagcttgg
      241 cacggacacg aacagtgtgg tgacgaaaat gatgatccga tccggccact tgccccggca
      301 cagggcggcg atggaaccca gcaccagacc ggccaccacg gcgatcagcg ccgccatcag
      361 acccagccgg gcggacacgc cgaaggattc accgatcacg tcgctgatct cgcggcccgt
      421 cttgagcgaa acacccaagt cgccgtgcag caggttgcgc agatagttga cgaactgctc
      481 gcccagcggc ttatccagac cgtagcgcgc ttccaactnn nnnnnnnncg tcgccgggtc
      541 gatggctttt tcgctggaaa acgggccgcc cggaatggcg ttcatggcaa aaaaggtgat
      601 ggccgcaatg atcaaaacgg tcaacagcgc cagtagaata cgcttaatgg agtaccgcag
      661 cattcccttc catcctttcc tggccggatc cccggaatca tcggaagatc cgccgcacag
      721 ggaaaccctg tgatttttca aattatagag acaacctgcc ggagtttcaa taaaaataca
      781 ctgaaaatac accaattctg tatggaaata caatcaaaaa acaaaattcg gtaatttgag
      841 cccttttctg cagttgttac aagtacttct attgacaaaa tgtggtagac agatgtaacc
      901 tattctcaga aagcggacgt accccttatt tcccgaaggg ttcacgccga ggaaaggagc
      961 attcaccatg cgtaccgctg ttgtctacaa ttttctgctg gaagcaaatc tgatggcttc
     1021 catcgcgatc ctgctgatgc tgcccattcg ccgttttctg agaggacggc tgggcagccg
     1081 ggccatttat tttgcgtggc tgctggtggc cgttcgtctg ctctgccccc tgaccctgcc
     1141 caacccggct atcaacggca tccgctctcc ctttgccatg gacagcgcca ttcgtcccat
     1201 cgccgggcag ctgatggtgc gctttcagga catactttcc cgggtggagc agctgatgga
     1261 ggaacatccc gccctgcccg gcagcgcggc tgtggcaaaa gacatgaacc atctattgga
     1321 aggcatgtat aatgggatgc tgcccctgac gttgatgaaa atttatctgg ccggggctgc
     1381 gctggtgctg atctggttcg cagccgccaa tctccgcttc cgtctgcggc ttcgggctgg
     1441 gcggatcgag cccatttccg ggcggctgct ggaacagtac gagcttctgt gccgccagcg
     1501 gaacgtgcgc cccattcccg tttattttac cgaccctctg cccagtgcct gtctggtagg
     1561 cgttttccgg ccctacatcg ccctgcccct gagcgcaccg ccccaggaag cggtgcaggt
     1621 gctgactcat gaggtgtgcc actacaaggg cggcgaccat ctgtgggcac tgc
//
LOCUS       DAWAEA010000317         1666 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_9222_length_1666_cov_1.843575, whole genome shotgun sequence.
ACCESSION   DAWAEA010000317 DAWAEA010000000
VERSION     DAWAEA010000317.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9222_length_1666_cov_1.843575"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          355 a          464 c          548 g          299 t
ORIGIN      
        1 cgtgctaagc tcccgaacct tcgagatcgg ctcggccggg atgcgggatc aggacgctat
       61 gcccctgctg cgggagggaa tgcggcggct tcagtcggca ttgaacgaaa cggtgcatct
      121 gggcgttcgc tccggcaatg agatgctgta tatcgacaag ctggactcca cccgtgcgat
      181 ccgcatgacg agccgaatcg ggacccgtgc gccgctgtac tgtacggcgc tgggcaaggc
      241 gctgctttcc ggcatgacgg acgagcagat tcgccgcctg tacgggcagg aagaactgaa
      301 agcctacacg gcccatacca ttacggacgt ggacagactg cttgagcaga tcggggaagt
      361 tcgccgaaat ggctacgcag tggaacggga ggaaaacgaa cccaacgtct gctgtgtggc
      421 ggtgccactg tgcagcgcgg cgggggaggc ggtctacgcc atgagcgtgt ccgcgccgtc
      481 ctttcgaatg gaggaaacag aattgaggcg ctgtgtggag atgctgacga gcgttcgcca
      541 gaagctcgaa ccctatctgc aaaccgtctg acggtttcaa gctggaaata ccactttatc
      601 gtttatgagg gaggaaagaa ccatggatat gatggaaaaa ctgtctctgg ccgggctggt
      661 gccggtcatc aaggtggaag acgcggccga tgcggtgccg ctgtgccggg cgctgtccaa
      721 gggcggcctg ccggtggcgg agatcacctt ccgcacggat gcggcggagg aagccattcg
      781 tcgggtacac gaggaactgc cggaggtaat gctgggtgcc ggcaccgtgc tgaccaagga
      841 gcaggtagac cgggccgtag cggcgggcgc ggcctatatc gtttcgcccg gccttaatcg
      901 ggagatcgtc cgttattgca gggaaaaggg cgtgccgatc attcccggct gcgccaatcc
      961 cagcgatata gaagtggcgc tggaggaagg cctgaagact gtcaagttct tcccggcgga
     1021 ggcactgggc ggcatacaga tgatcaaggc catgtccgcg ccctatggcg gcgtgacctt
     1081 cctgcctacc ggcggtatta acgaaagcaa tttgaatgat tatttgtcct tccccaaggt
     1141 cgtcgcctgc ggcggcagct ggatggtccc gcaggatgcc atcgccacga aggagtggga
     1201 acgcatcgag cgcctggccc gcagcgccgt caacgccatg ctgggactgg aactcaagca
     1261 cgtgggcgtg aacagcggcg acccggagac tgccgcgaag gacgcggcgc agttcgcacg
     1321 actgctgggc tgggagctgg atgaacagcc catcgccacc tttgtgggcg gcggttttga
     1381 gatcatgaag atccccttcc ggggcaagaa cggccatgtg gccatcggct gcaacaatat
     1441 caagcgcgcc cgctggcaca tggagcgccg cggcttcgtc ttcgacgatg acagcgtcat
     1501 catgaaaaac ggcaagctgc gcgccattta ccttaaggac gacatcgccg gttttgccgt
     1561 gcatctgctc caaaaataag gcgtacaacg cacaaacagg aaaggaaatc agcccgtgaa
     1621 ccagaaagtc gttaccttcg gcgagatcat gcttcgcctg tctacg
//
LOCUS       DAWAEA010000318         1661 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_9246_length_1661_cov_3.105230, whole genome shotgun sequence.
ACCESSION   DAWAEA010000318 DAWAEA010000000
VERSION     DAWAEA010000318.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9246_length_1661_cov_3.105230"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    402..421
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          301 a          535 c          494 g          311 t
ORIGIN      
        1 tttacgcggt caagggcaac aacgattacg gctgggaagc gcccgtcacg caggtgatct
       61 ccgtgggcaa ggcgaagctt ctgctctgcc acggccacac cttccatgta aagagcagcc
      121 tttaccattt ggaatttgaa gccgccgatc aggacgttca ggccgcctta ttcggccaca
      181 cccatttagg ccatctggaa tacgccaacg gctgtctatt cctgaacccg ggcgcagtct
      241 gcaactacgg ccccaatctt cccgtctatg ccgagcttct cgtctccgac gacggttcag
      301 tccatgcatc tctacagcag ggctaaagtc ctgcacccac ttctgcgcgc atagcgcgca
      361 ggggcgtagt gggacatgcg gcttggccgg ggttttaccg cnnnnnnnnn nnnnnnnnnn
      421 nggctaaagc ccttgaccca tcaccgcgcc catagggcgc ggggctactc gaacgacatg
      481 cggcttggcc ggggttttac cgccgtggca caacaggtgc cacggctcgc cggtctggca
      541 gggcgagttg ccgcttgacc cccggcagcc gccccatgtt ttacaaaacg ctgccacatt
      601 ttcccgttct catgcgtctt ataaggggcg caattgtcga ttttcgtcag gctttccatc
      661 agaatcgagg ttttgctcat gagtcccttt atttatctgg acaacgccgc caccacggcg
      721 gtcaggccgg aagtcctttc caaaatgctg cccttcttct gcgacagggc gggcaatccc
      781 agctccattc acgcagctgg gcgagaggcc cgcaaggcgg tggagaacgc ccggcggcag
      841 gtggccgcgg cgcttcatgc cgaggcccgg gaaatttact tcaccgccgg gggcagcgaa
      901 agcgacaact gggccatccg ctgcgctgca gcagagacgg gcaaaaagca cgtcattacc
      961 tccgccgtcg agcatcacgc ggtgctgcac agctgccgcg cgctggaacg ggacggcttt
     1021 cgcgtcacct atctgccggt ggacgaaaac ggtctggttt ccccggacga cgtccgccgg
     1081 gcgctgacgg acgacacggc cctgatttcc atcatgacgg ccaataacga ggtcggcacg
     1141 ctggaacccg ttcgagagat cggtcagatc gcccgggagg cgggcgtgct gttccacacc
     1201 gacgcagtgc aggccgccgg ggccgttccc atcgacgtaa acgactggaa cgcagatctg
     1261 ctttccctct ccggccacaa gtttcacggc cccaagggcg tgggcgcgct gtacatccgc
     1321 aaagggacgc gcatccacaa cctcatcgag ggcggcgcgc aggaacgggg gcttcgggcg
     1381 ggaacggaaa acgtgcccgg catcgtgggc atgggcgatg ccctttcgct ggccgccgcc
     1441 gagctatctg aatacacggc ccatgttacc gcgcttcgcg accggctgat tcacggagta
     1501 ctgtccgccg tgcctgacgc acggctgaac ggccatcctg tcctccgcct tcccggaaat
     1561 gcgaattttt cctttcccgg ggtggagggc gaaagtctgc tcatgcggct ggacttggcc
     1621 ggaatcgcct gttccagcgg ttccgcctgc accagcggca g
//
LOCUS       DAWAEA010000319         1661 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_9250_length_1661_cov_1.084682, whole genome shotgun sequence.
ACCESSION   DAWAEA010000319 DAWAEA010000000
VERSION     DAWAEA010000319.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9250_length_1661_cov_1.084682"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    715..906
                     /estimated_length=192
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1274..1283
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          264 a          492 c          401 g          302 t
ORIGIN      
        1 cggcagggct ttgacgcggt ttacgaccgc accgtccgct ggctgtccgc ccgggaaaag
       61 cctcttgccg acccgaaact tcacgagctt ctgaacacca atctgttctt cgcctttttc
      121 tacgcttccg gccgcaccgt cgacacggag gaattgtgcc tgatgaccag ccgaagcccg
      181 cgctactacg tctccgccgc gtactgggat cgggacagtc tgctgtgggc cttcccggcc
      241 attctgacgg tggacgcggc ctatgcccgg gaaatcctga cctacgtgtt cacccgtcag
      301 gcccggaatt tcggcgttca cagccgcttt atcgacggaa ccatgctgga acccggcttc
      361 gagctggacg agctttgcgc gccggtcatc gccctggacc gctacgtgga aaagaccggc
      421 gataccgctt ttgcggcccg gccctttgtg cgggacggtc tcgaagaaat tctacgcaga
      481 atggaggcca aaaagcatcc ttccgtccca atgtatgaga ccttcctgca gcccacggac
      541 gatatgcata accatattta cctgacctac gacaacgcac tggtgtgcta tgcgctgcgg
      601 gcgctggcgg agctgctgaa ccgtccagag ctggcggaag aagcggaacg cacccgccgg
      661 gcagtttacg aacactgcgt cacggagcag aacgggcggc ccttcttcgc gtggnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnncgag agctatccgc tgtccttcgc cggacacccc attgcggaga tcggctgcaa
      961 gcacgcgccc catccgtgga tcctttccat ctgcaacagc ctactcagtg gccacgcgga
     1021 cagcgctctg ctgcacctaa gccgcacgcc gctggataac ggcgttgcct gtgaaagcgt
     1081 caacgaagat accggccttt gcgagaccgg cgaagccttc gccacctgtg ccggtttcct
     1141 gagctacgca ctgtactgcg cctgctgcaa agactgatct ttatcattac agcaaaccgc
     1201 cccggcaatg ccggggcggt tttttgtatg ttttttttca ccgttcccga tcaggccatc
     1261 cgctgctgta ttcnnnnnnn nnnggtcgat caccgcaagg ttctgctgct gcgccgataa
     1321 atcgggattc tgcactcccg cccgccatgg agtcccgtcg ccttttcgcc cgatggagcg
     1381 cagattgccg ccgatgctca tcagcaggct gtccacgccc tgcgcttcca ttttccgcgc
     1441 tgtcaactcc accgcatagc ccttggcgat ggctcccacg tccagcgaca tttccgggtc
     1501 ggccagataa acggtactgg cgttcggatc cagcaccatc tggtcaatat ccgtatgttc
     1561 gcccgcccgg tacagctcct ccgccgaggg aaccgccgcg tgttccggat cggccagccc
     1621 cagctcccgg tattcgtgcc agatggaaag cacgctgccc a
//
LOCUS       DAWAEA010000320         1655 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_9287_length_1655_cov_1.458125, whole genome shotgun sequence.
ACCESSION   DAWAEA010000320 DAWAEA010000000
VERSION     DAWAEA010000320.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9287_length_1655_cov_1.458125"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    295..385
                     /estimated_length=91
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          378 a          494 c          434 g          258 t
ORIGIN      
        1 aaacggcgaa tacagcttca acgtccagaa ggacgcccat cagtggggcg accgggcttc
       61 cgacagcctg aaggtgatcc tggacagcgc catcatctac aacgaagccg gcgctgaaaa
      121 gcgtgaccgg gatcagatcg tggctgtgaa cgccctggac gacgtgaccg cgggcgacgg
      181 aaacatggtg gacggctatc tctcccgcag cgacatcgcg ggcggcatga agcagatcat
      241 ggcccaccag agcgacgaaa cccctaacga aaaggtgacc gatgctatcg aagcnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnntgctg gcactgaccg atgacaatgc catcgaatac
      421 acctttgaaa cctacaagaa tggcgtcaag acccagatca ccgaaaccct ctacgctcat
      481 ggcagcaaca tgatgcccta cgccgaaaag acccccgacg gcgtggacgc agcatccttt
      541 cctgatcccg attgggaaat gacttactat gtggcggaag gccagaccac cgaaaacggc
      601 aatcccgtgg tgaccgcgga taagcctgcc ggcgcttccc acatcctgac cgtgcaggat
      661 ctggcgggcg tggacatcta caccgaagaa gggttggagc tctttgataa gctggcctcc
      721 atgaccacca tcgacgaagc cattgaaatc cagggtaact ccggttggaa cgtcagcgct
      781 gtggacagcg tgggcaagcc tgctcagaag gctcaggacg gccccggcga acccgccaac
      841 ggcaccttcg ctggcgcgac ctggttcccc tgcgctgtga ccatcgccgc cacctggaac
      901 aacggcctgg cccgcgctga gggcgtggct tacggacatc aggccatcct ggctggggtc
      961 ggcgtggcgt atgcgcctgc catgaacatc caccgcagcc cctttggcgg ccggaacttt
     1021 gaatactact ccgaggacgg cttcatctcc ggccacatcg gcggcagcgc catcgaaggc
     1081 atccaggcca ccggcaccaa tgtgaccgcg aagcacgcgg ccctgaacga cggcgacacc
     1141 aaccgcggcg gcaacaccac ctgggccaac gaacaggcca tccgtgaaat ttacctcaag
     1201 acctatgaaa tcgcctgtga ggactacggc tgcaacggca tgatgggttc tctgaaccgt
     1261 atcggcatga cctggttcca ctatggcatg tacaaaacca tcatgcgcga cgaatggggc
     1321 tggcagggcc tgctgatcac tgacggcgac ggtatgggcg gcgacgctta caataccccc
     1381 gttgcgatgc tctgtgtgaa cggcaacatg ctctcccgca gcctgtacat cgacgcccgc
     1441 gccaccgttg ctgcatatgg cgacgccacc gattatgtct atggccgcta catgctgcac
     1501 aacaacatcc gcaatgccct ctaccagtac gccagcaccc tgaataacgc tgaataagaa
     1561 ctgatacctg ctgcgcggcg aaaccccggc ttaaaacgcc gcgcagcttt tcaatcaata
     1621 gaaggagcaa atgaaagatg aaaaaagttc tatcc
//
LOCUS       DAWAEA010000321         1654 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_9293_length_1654_cov_1.691057, whole genome shotgun sequence.
ACCESSION   DAWAEA010000321 DAWAEA010000000
VERSION     DAWAEA010000321.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9293_length_1654_cov_1.691057"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1252..1271
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          334 a          508 c          416 g          376 t
ORIGIN      
        1 gcatattata gcacatattg cattcttgta aacaaaaaga cgcatttttg ccttatttgg
       61 gagacctgtc cagaaaagaa aacaaaaaag acgaacagcc gctctgtttc gctgtccgcc
      121 ttgtccacgc tgaaagccat gcgggtcaaa accgcttaat ccagcacaat agcctttcgt
      181 ttgccgtttt ttcctacggg atattcccga agccgaatga caaaatccag attaacgacg
      241 gtttcctttc cgtccttttc aataacggcg gcattgtccg ctatttcttt caaaatcccg
      301 tccaccgttc ccgaagagat cgtgctggcg atcacatcct tgccaagaaa ccgctttgca
      361 agctcctgca tttcgttatc tccgtttctt cttttgattc tcgtttccag atatttcgcc
      421 gattcctttc ggttcaggaa aagaatcaga aaaatcagca ggatcggaaa agaactcatc
      481 caccaagtct gcataccgtt cctctttccc ctgtctttac tttgccaaaa aatccgagat
      541 tagtgcgcag gcttcctcca ccggctgctg atccatctgg agccagggaa tagccccgtc
      601 ccggcggaac cacgtcatct gccgcttggc aaactgcttg atagccgtgg ccaacagccg
      661 gttcagttcc tcctcgctgg gaatcgcgcc ggtcagatat tgggtgatga gcttgtattc
      721 cagccccagc ttcagtagaa attcctcgct gacgccggat tccatcagac cgcgcacctc
      781 ctgaatcatg ccctcctgaa agcggcgggt catgcgctcg tcgatgcggc ggcccagcac
      841 ctcccggggc caggtcacgc ccaggcgaag acagtcgtac cgaggatgtt tggtaggaac
      901 cgcgtcgtcg ccgtcatgga gcttttccag cacccgcatg acccggttgc ggttgttggg
      961 ctccacgtcg ctctgtggac atttctgcat cagcagggcg taaagctccg ccgtggtcag
     1021 cttttccagc tcccggcgat agctcagatc gggcattttg ccggaaagct gatacccatc
     1081 cgccactgcg ttcacatata gcccggtacc gcccaccaga aaggggactt ttccccgggc
     1141 cagaatatcg tcgatggcct cataagccag ccgctgaaaa tccgccatgg agaaaaaatc
     1201 gcccggctcc accacgtcga tcaggtggtg aggcacgccc cgcatttcct cnnnnnnnnn
     1261 nnnnnnnnnn nggccacctc cacgcccagt gcgctcttgc ccgaggcatt ggtgcccgcg
     1321 acgacgatca gctttccttt cgcctccggg gagcgctgat aggcgatgcg ctctgtgccg
     1381 tcctgcatcc acacaatgcc gcagcggcgg aagccgttgc gctcgaaggc ccgctgcatg
     1441 ggcagattgt ccgcgtgggt gtcgccccga aggttctcgc agcgtttcag cgcccactgg
     1501 acgatgaagt ccaccatgcc cttccgttcg ccagaggacg ccacccggtg cagggtctga
     1561 tagggcaggt cgttgagcca cgccccgttg atgcggcggt agttggggtc ttctccctcc
     1621 agcagcatga aggcggcgta cagatggccg tttt
//
LOCUS       DAWAEA010000322         1650 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_9315_length_1650_cov_1.653918, whole genome shotgun sequence.
ACCESSION   DAWAEA010000322 DAWAEA010000000
VERSION     DAWAEA010000322.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9315_length_1650_cov_1.653918"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          318 a          450 c          521 g          361 t
ORIGIN      
        1 ctccgggttg atggaatcat agcccagcag ggtaggggcg ctcatggcgc acaggccggt
       61 aatgaaggtc atgaccgtca ggctgaacag ggtggggatc agggtgggga aaaccacctt
      121 catcagcacc ttgaagggct tcgcgcccag attgcgggcc gcttccacgg tgttgtagtc
      181 gatgccgcgg atggcgttgc gcaggaacag catatggttg gaggtacagg cgaaggtcat
      241 ggtgaacagc accgcccgga acccggagaa ccagtccttg ttgaagcccg ggatcacatt
      301 cgccagccag gtagtcagca tcccgttgga atcatacagg aacaggtagc cggtcaccag
      361 cgccacgccg gagtagatca gcgtggtcat gtagcccagc agcagtacct tcgcaccctt
      421 gatgtcgaag tactcggtca gaagcacgat actgatgccc acgaagttca ccgtgatgac
      481 cagcgaaatg gccagcttca gggagttcca tacatagctg ggcatgttgc ccgcaaagaa
      541 gaagcggatc acggccagcg ggtcggtctt gccgtctgca cccttgacgg tgaagatctg
      601 cgtgatggtg ttcaggcagg gaagcagcat gaagccgaag aacagataca cgaaaaaggc
      661 gatgccgaag accttccaga tgtactccgg cttcagccat gaggctttct ttgtcatggt
      721 tccgccccct tacgcttcgg cgggcgcatc gtaggacatg acgtccgacg gatggatgcc
      781 cacggtgacg gtctcgcccg gctcgtagat gttgatgccg tcgttctttt cgatggcctt
      841 gaggatctgt ccgtccacgc ggatgtagta cttgatgtac aggccgtagt attcccggct
      901 ttcgatcaca ccctgcagct gcagctcatc gtccgccttg ggccggttca cccgcagacg
      961 ctccagacgg atgaagtggt gctggttttc cggcagcttc atttcgccgc agatggtctc
     1021 ctccagccgg ttgatgtcgc cgatgaagtt gcagacgaac tcggtcttgg agtgattgta
     1081 aatttcattg ggtgtgccga tctgttcaat gtagcccttg ttgaacacgg cgatgcggtc
     1141 ggacagggtc agggcttcct cctgatcgtg ggtgacgtag atggtggtaa tgccgaaatc
     1201 ccgctgcatc tttttcagct cgttgcgcag ctgcacgcgc agcttggcgt ccagattgga
     1261 caggggttca tccatgcaga tgatggaggg gcccgtcacc agcgcgcggg cgatggccac
     1321 gcgctgctgc tggccgccgg agagctggct gacggctttt ttcagctgct cgtcggaaag
     1381 gtctaccttc cgggcgatgt cccgcacctt ctggtcgatc tcggtcttgt tcagcttctg
     1441 aattttcagg ccgaaggcga tgttgtcata caccgtcatg gaggggaaca gggcgtagct
     1501 ctggaacacg atgccgatat tccgcttttc gatcggcacc cgggtaatgt ccttgtcgtt
     1561 gacgctcacc gtgccgctga ccggctcgat gaagccggtg atggtgcgca gcgtggtggt
     1621 cttgccgcag ccggaggggc cgagcagcgt
//
LOCUS       DAWAEA010000323         1647 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_9336_length_1647_cov_1.273241, whole genome shotgun sequence.
ACCESSION   DAWAEA010000323 DAWAEA010000000
VERSION     DAWAEA010000323.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9336_length_1647_cov_1.273241"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    549..762
                     /estimated_length=214
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          285 a          458 c          404 g          286 t
ORIGIN      
        1 aggctatcac ctggtgaacc agtacattac gccgtccccg tcgcccagca cgaccccgag
       61 tcccacgccc gaaccgagtg taacccccag tcccacgcct acggccacgc cgagtccgac
      121 cccgagtccc acgcctacgg cgccttatac acccccgaaa accggggatg aatcgccgat
      181 ggccctgtgg ctggccttgc tggccgcaag cgtcgtcctg ctgggctgga gcctgcggaa
      241 gcgtaagcac ggataagaaa tttcattttc aaaaaatcaa agcccgggcg gttcttctgc
      301 cgtccgggtt ttctgctgca aaaatgaaga agaatggcgg gagaggcgca ggtcttttcc
      361 atctgcgcgg gcagtgctat tccccaccct gctgccagca ggagcagccc ggcccgtagt
      421 ccgggcggga atgggtcgtg accagaatca catcgtcgcc gatgcggcgt acatcctccc
      481 aaggaatgac cagaccgcat ttttcgccgc gcagggattg cagaaaggaa gtctcggacg
      541 gcaccaccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntcccaagg aatgaccagc
      781 ccgcattttt cgccgcgcag ggattgcaga aaggaagtct cggacggcac caccagcgcg
      841 gtaatgcggc tgtcctccac gctgaattcc agatccatca ccttgcccag ccgccggccg
      901 tcctgcacgt cgatcacctc tttggtgcga agctctgaca gcgtcaaccg tctgcctcct
      961 ttcttaccat gcgtttatgg gggagtacta acagcctatg cgccgaaaaa cggaaatgtg
     1021 cgctctatgc caaaagaccc cgtcctgccc ggagaagaac ggacaggacg gggttcgtca
     1081 gcagcgatta tgcggcggtt ttggattttg aagtgtgatt tttacggctt tttccgctcc
     1141 cgcagggctt tccagtccac ccgaccgtcg ggcagaaagg ggacgccttc cccttccaac
     1201 agggcgcggc gtagttcgga ttgcccgccg ccggcaatgg agccgtccgc cttgacaacc
     1261 cggtgccacg gaagttcctc agggcagcgg cgcatggccc agcccaccat ccgcgccgcc
     1321 cggggacggc ccagcatttg ggcgatctgg ccgtaggaca gcgcctgccc cttggggatt
     1381 tgcgccacaa tggcgtaaac ctgctgaaaa aatccgtttt tcggttcgga atttgctttg
     1441 cttgccatat ttttacaatt ctaacttttt tattttgtca gcccatccag ataggcccgc
     1501 agccgggcaa cgtcgccgtc agcaaagcag tagcccgtca tgccggtgct ttccgcgccc
     1561 tgaacgttgg cggcgctgtc gtcgatgaac aggcattcct ccggcttcag gctgaatttg
     1621 tcgcacaggc gcaggaaaat gcgtggg
//
LOCUS       DAWAEA010000324         1646 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_9339_length_1646_cov_1.754871, whole genome shotgun sequence.
ACCESSION   DAWAEA010000324 DAWAEA010000000
VERSION     DAWAEA010000324.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9339_length_1646_cov_1.754871"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          353 a          519 c          493 g          281 t
ORIGIN      
        1 gtggaaacgg gcctgtccga tgaaaactac gcgcaggtat tgagcggcct tgacgaaggc
       61 gacgtggtgc tcattacccg ggaggcttct tcttccagca ccgacagccg ggatggcggc
      121 tttgacttcg acagtatgag cttcgacatg ggcagtatgc ccagcggcgg cgacatgccc
      181 agcggcggct tccccggcgg gggcggcgga agcggcaacg gcggcggccg ttccgacaac
      241 cgttaaggag gcttttccat gatcgaaatg caggacatcg taaagcaata cacgctgggc
      301 ggcgagatca tctacgccct cgaccacgtt tccctgcggg tggaaaaggg cgaatacgtc
      361 accatcatcg ggccctccgg cagcggcaag agcacgctga tgaacctgat cggctgtctg
      421 gacacggcgg acagcggcac ttacgataag ctgaaaaaca agcccaacca gctttccggc
      481 ggccagcagc agcgctgcgc catcgcccgg gccattgccg tcaacccgtc gctgatcctt
      541 gccgacgagc ccaccggcaa tctggatcag aagaccggcc gggaaattct gaacattttt
      601 gacgagctgc atcaggccgg gaataccatc gtcctcatta cgcacgacct caaggtggcc
      661 ctctgcggcc aacggatgat ccgcattgag gacgggcggc tctacgaacc gaaggaggcg
      721 gacgcggatg atctttcagt cgtttaagat ggccctgaag gccattgccg gcaataaaat
      781 gcgctctttt ctgaccattc tgggcgtggt gatcggcgta ctggccatcg tggtgctggt
      841 ctccatcggt cagggggcca acagctccgt caccgccagt atcgagggca tgggcaccaa
      901 cctgatctcc gccaccatca acgcccggcg gacgaacccc gtcacagccg acggcctgaa
      961 cgagttggcg gaaaacgaag ccatcgccta cattgcgccg ctgaccaacg tttccggaac
     1021 ggttaaggcc ggcgtgaaca cctatgacga cgggacggtg cagggcacgg ctcccggcta
     1081 cgagcacatc cgcaactgga cagtggccga agggcgcttc ctgacccagc ccgacgtcga
     1141 caaccgcagc tttgtggcgg tcatcggcgc ggaggccgcc agcgaaatgt acggaaccac
     1201 ccacgctgtc ggcgagacct tcaccctcag cggctacacc atgacggtgg taggcgtgct
     1261 gaaggccaac ggtgaaagcg ccagcggcag caacgacaat caaattctga tcccctacac
     1321 gctggcccag cggctgtcca actccacggg catttccagc ttctacgttt ccgccgcatc
     1381 ctccgatcag gtgacacagg cgcaggcggc ggtggaagcc tatctggaaa aggcctttga
     1441 gaactacaac acccgttcct tcggcaccca gtacagcgtg tacaaccaga cggaaatgct
     1501 ctccaccctg agcgacacca cggccaccct ttccctgata ctgggcggca ttgcgggcat
     1561 ttccctgctg gtgggcggca tcggcattat gaacatcatg ctggtcagcg tcagcgagcg
     1621 tacccgggag atcggcattc gcaagg
//
LOCUS       DAWAEA010000325         1638 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_9390_length_1638_cov_1.492104, whole genome shotgun sequence.
ACCESSION   DAWAEA010000325 DAWAEA010000000
VERSION     DAWAEA010000325.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9390_length_1638_cov_1.492104"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    687..696
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          371 a          478 c          370 g          409 t
ORIGIN      
        1 tgcatgaccc attttttatc ttcccagttt cggttggcta taataggtat ttgtccttgc
       61 tgcggaattt tatcaaataa aatattgcat cccacccaac cggctctttt cgccgtagaa
      121 gccagtggtt tgcgtcgttc aaccatatca ggtacaaaaa aatttttggg aatgatccac
      181 aactgatcaa cgatcaattc tttcggatcg taactcataa tcaaaaaatc cggattatcg
      241 ttgcttgtga ttcgttgaat aaaagactca taagctccat cggcaatctt ttgaccaatg
      301 tttccttttt tactctttag ttcatattga ttttggcatt cagggcaata aaaatcagca
      361 acttttctgt tattagggaa atgcataatt ttcggatttc cacaccttgg gcaataaata
      421 tgctccccta tccatgtttc cgtcaatatt cttgcttgtt gagaatggct tctatactct
      481 gcgcccaatc ctgaattcag ctgaaaatct atcgtactca tctctatctc ttattttcgc
      541 tttctttcgg aaacaacctc acgctctcct cgtccataaa ggcgggggta taggcgctgt
      601 ccgccgcttc cttttcctgc agatagccgt ccagccccag ctccatcatg tgctccaata
      661 cctgccggta ttcccggccc gtcaggnnnn nnnnnntctg tgcgcacatg gccttcaccg
      721 ccgccatcgc cacctcgaaa taatccggtg cggcagccag cgcggcggcg cttttttcgg
      781 aataatactt tagatcgggc aggaagatat ccaccagtcc ccgggcattt tccaccgttt
      841 ccagcgtttc ataaccgctg gaattccaca ccaccggaat gcccggcttc cggatggaaa
      901 gagcctcgaa aatcgccggg gcgaaatggc tgggcgtgac cagattcaga ttatgcccgc
      961 ccgtttcctc cagccgccgg aacaattccg ccatctggcg gggcgtcatg gcccggccca
     1021 gctcgtgatg gctgatgggc tcgttctggc agaacacgca ccccagcgag cagccgctga
     1081 aaaacaccgt accgctgccc cgcgtcccgc tcaggcaggg ttcctcccag taatgggctg
     1141 cggcgcggga caccaccggc agcgtcccca tatggcagaa gccttttccc tcgtgttccg
     1201 tgcgttccgc gttacagtgg cggggacaaa cgttacattt catgctgtca acctcccaat
     1261 tcggcggctc cggggccgcc ccttgccatt cagctcaaaa ttggttataa tcggaatgac
     1321 gaacccgtca aaccttagga aacggaggaa cccatcatgc tggaattcaa atttgacacc
     1381 cagcttctca tcgagggcca caacctctcg gaggacgcca tcaacgaata catcaccaac
     1441 aacctcaagg gcgactgtct gctggctgtg ggcgacgagg atcttatcaa gatccatttc
     1501 cacaccaacg agccctggca gctgctggaa tacgcttcct ctctgggcga gatctacgac
     1561 atcgtcgtcg aaaacatgga gcgtcaggaa aacggtctgc agggctagca ggggcagtgc
     1621 ccctgcaccc cggactgc
//
LOCUS       DAWAEA010000326         1637 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_9394_length_1637_cov_2.512642, whole genome shotgun sequence.
ACCESSION   DAWAEA010000326 DAWAEA010000000
VERSION     DAWAEA010000326.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9394_length_1637_cov_2.512642"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          315 a          491 c          565 g          266 t
ORIGIN      
        1 ggtggacgac gagacaaggc cccatacgct gcgcggcacg ctggaattcg cgtttgacca
       61 atgcactcct gttccgctgg aagaggtgga gcctgcttcc gagattgtga ggcgcttcaa
      121 gaccggagcc atgtcctacg gctctatttc gcaggaggct catgaatgtc tggccgaggc
      181 catgaaccgg ctgggcgggc gctccaattc cggcgagggc ggcgaaagcg aagaacggct
      241 gaacacggag cgcggctcca agatcaagca ggtcgcctcc ggccgcttcg gcgtgaccag
      301 cgaatacctg atgagcgccg aggaaattca gatcaaaatg gcgcagggag ccaagcccgg
      361 cgagggcgga cacctgcccg gcagcaaggt ctatccgtgg attgccaaaa cccggctgtc
      421 cacccccggc gtgagcctga tttccccgcc gccgcaccac gatatttact ccattgaaga
      481 tttggcgcag ctgatctacg acctgaagtg cgccaacagg cgggcgcgga tctccgtcaa
      541 gctggtcagt gaggcgggcg tgggcacggt ggccgcgggc gtagccaagg ccggggcaca
      601 ggtgatcctg atctccggca gcgacggcgg caccggcgcg gcccaccgca cctccatcca
      661 caatgcgggt ctgccgtggg agctgggcgt ggcggaggct caccagaccc tttcgctgaa
      721 cgggctgcgc tcccgggtcg ccatcgaggc cgacggcaag ctcatgagcg gccgggacgt
      781 ggcgatcgcc tgtatgctgg gggcagagga attcggattt gctaccgcgc cgctggtctg
      841 catgggctgc gtgatgatgc gggtgtgcaa tctggacacc tgccctgtgg gcatcgccac
      901 ccagaacccg gagctgcgca agcgcttcaa gggtaagccg gaatatgtga tgaacttcat
      961 gcttttcgcg gcggaggagc tgcgggagat catggcgcgg ctgggcgtac gcagcgtgaa
     1021 ggaactgatc ggccggggcg acctgctgcg ggtacggcag catcaggtta cccgccgggc
     1081 cgcgtccatc gacatgagtc ggatcgtcgg ccggtgcatc aacgaatacc gacgcccgga
     1141 ggacaccttc gacttccata cggaaaagac ggtggacgag cgggtgctgc tgaaaaagct
     1201 gaacttgaaa agtgtgaagc cgcagagcgt cgagctggac gtttccagca cggaccgggc
     1261 cttcggcacc attttcggca gcgaagtgac ccgcgtccac ggcacgtcgc tcaaggagga
     1321 tacctatacc atccgggcag cgggcggcgg cggacagtcc ttcggcgcgt ttattccgaa
     1381 gggtgtgacc atccgtctga ccggcgacag caatgactat ttcggtaagg gtctgtccgg
     1441 cggcaaactg gcggtccgcc cgccggaaaa cgcccgcttc cgggcggatg aaaacatcat
     1501 catcggcaac gtggcgctgt acggcgcgac cagcggacag gcctacgtct gcggcgtggc
     1561 aggcgagcgc ttcgccgtgc gcaactccgg cgcggtagcc gtggtggagg gcgtaggcga
     1621 ccacggctgt gaataca
//
LOCUS       DAWAEA010000327         1636 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_9407_length_1636_cov_1.380139, whole genome shotgun sequence.
ACCESSION   DAWAEA010000327 DAWAEA010000000
VERSION     DAWAEA010000327.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9407_length_1636_cov_1.380139"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1039..1197
                     /estimated_length=159
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          299 a          423 c          439 g          316 t
ORIGIN      
        1 cttccgtcct gcggattctg gaattgaaga atcggctggg gctgatggac gattcccggc
       61 agccgcagcc cgcgcctacc gcggagcagg tgtctgaaaa ccgccgcaaa gcgcttcagg
      121 cggcgctgca aagctgcgtg ctgatgaaga acgacggtgt gctgcccctg aaacccggtg
      181 ttcacttggc gctggccggt tcccacgcgg acgaacacgg tctgctgggc ggctggcaca
      241 cggatggccg caacgaggaa acgccctccc tgctggatgc gttccgggcg gaaaagaacg
      301 tgatcctctg caagcctgaa gaagccgatg tgatcctttt tgcctgcggc gagcagcagg
      361 atctgaccgg cgaggcctgc agccgggcct atcccaagct ggaggaagaa cagctgcagg
      421 agctttacgc cctgtccgaa ctgggcaagc ccatcgtgat ggtgctgttc tgcggccggg
      481 cgctggtgct gtcggatgcg ttgcccttct gcaatgcctt ggctccgccg cgggctatga
      541 tcaggtggtg cagttcccct tcggcttcgg cctgagctat acgcagtttg aaatttcgga
      601 tatttgcgcc gatactgacg tgattcaggc aaatgagccg ttccatgtga ccgccaaagt
      661 ccgtaatgcg ggcaaccggg ccggagaaac ggtcatccag ctctacagca acgttcatgt
      721 gccaggccag cttacgccgg tgcgttccct gaccgactgg cagcgcattt gcctcggcgt
      781 gggtcggcaa atggaagtga ctttcgaact gacccttgcc cagctttccc agctggacgc
      841 tgaaggtcgc accgtaacgc cacgtggaaa ggtgcgcatt gctgtaggcg aggacagcca
      901 cgcgccgttc acagtgacgc tccccgtaga ggcgtaaggg aatacaacaa aatcccgaca
      961 gagccattca aagctttgcc gggattttgt ctgttgcggc gaaattatac gcagtgaaag
     1021 atcatgttga cctcgtggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnctg
     1201 aaattttcgc agcaggacga aacctaccag ttggttttca tcccaaatgt caaaggcgac
     1261 ggtgtcctcc cgctgcacgg cccgcccgat catctccggc gtgcccaccc attcccgctg
     1321 ttccggcagc acggacacct tgatttcact ggagggggtg aaggtcataa ggaaagccct
     1381 tctttctgat ctgtttggtg caatgctgaa aatacagttc ttaagctcgc ttcttctgct
     1441 gcttttgatt gttctctgcg atccgttccg agaaagaaag cggtgccgcg cgtctgccga
     1501 gctgctccgt aaagcggcgg gaatacgcga tataaagacc gtacacatat ggcattccca
     1561 gattggtttc cagaagcgaa ttgacgacga gcgtatggag cttttctcct gccgtggcaa
     1621 agcccgcgct gcggat
//
LOCUS       DAWAEA010000328         1633 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_9423_length_1633_cov_2.031686, whole genome shotgun sequence.
ACCESSION   DAWAEA010000328 DAWAEA010000000
VERSION     DAWAEA010000328.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9423_length_1633_cov_2.031686"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          285 a          478 c          531 g          339 t
ORIGIN      
        1 aatttgcctt actcggtcag ccgtccgacg acctcgtaga gatgctccaa ttcttcggag
       61 ctgttatagg aaagggtgat cttgcccttt ttctcgttgc cggtcagcac agtcttgagc
      121 cccaacgcct gccgcatggt atcctgcagg gtgtgaagct cgggggtcag ggggcgggga
      181 gcggcggatt tcttcaccgc cggggccgcg ggttcttccc tggcaagctc ttccagtttg
      241 cgcacggaaa gggattctga tacgcagcgc cgggccaacg cgatctgctg ctcggcggag
      301 tccagccccg ccagtacccg ggcgtggccc gcactgagtt cgccggactg caccagcgtc
      361 agcacctgct tgggcagggt gagaaggcgc agggtgtttg cgatggcggg acggctcttg
      421 cccagctttt gggcggcctg ctcctgcgtg tagccgcatt cgtccatcag ggaacgaatg
      481 gcggaggctt cttcgatggg gttcagatcc tgccgctgaa ggttttcgat cagggcggcc
      541 tccatctgct gctcgttggt caggtcgcgg acgatgcagg gaacggtgga aagccccgcc
      601 agccgggaag cccggtaacg gcgttcgccg gctacgatgc ggtagcgcat tccgtcctgc
      661 accaccagaa tgggggagag aacgcccgcc gagcggatgc tttccgccag ctgttccagc
      721 gcttccttat cgaaatcctt acggggctgg gcggtgttgg gatcgatgtc gtcaatggcg
      781 atctcctgca ccacggtttg gaaaagctcg tcattctggg gcagcaggac gtccagtccc
      841 cgtcccaatc cggaacgctt catggttttc acagactcct ttgcggccgg caatccttac
      901 cgattgcggc ggatgatctc ctgcgccagc tggcggtagc tttccgcgcc ctgactgcgg
      961 ggatcgtaaa gatgaatggg aacgccgtgg ctgggggctt cgcccagacg cacgttgcgg
     1021 gggatggcca cggaaaagac cttatcccgg aaatgcttct tgacggattc caccacctgc
     1081 aggctcaaat tggtgcgccc gtccagcatg gtcaaaagca cgccctcgat ctccagggag
     1141 ggattgaagg tatgctttac ccggctgatg gtgttcatca ggctggatac gccttccagc
     1201 gcgtaatact cgcactggat ggggatgagc acccgctggc aggcggtgag ggcgttgagg
     1261 gtaaggagac ttagggacgg cgggcagtcc acaaagacga aatcgtaatt gcgttccagc
     1321 cccatgagga cgctcttgag gcggtattcc cgctgatcca gatccaccag ctccagttcg
     1381 gcaccggcca gacggatgtc ggcgggcagt acgtccaatc ccttgacggc ggtgtgcttc
     1441 aggcaggcgt tgaccggcgc gcggcccatc agcacctcat agatcgaggt ggaacctgcc
     1501 tgcagaccca gaccgctggt ggagttgccc tgaggatcca gatccaccag aaggaccttt
     1561 ttgcctgcgt ccgccagaca ggcggtcagg ttgatggcgg tggtggtttt gcccacgcct
     1621 cccttttggt tgg
//
LOCUS       DAWAEA010000329         1630 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_9446_length_1630_cov_1.800635, whole genome shotgun sequence.
ACCESSION   DAWAEA010000329 DAWAEA010000000
VERSION     DAWAEA010000329.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9446_length_1630_cov_1.800635"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    428..563
                     /estimated_length=136
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          320 a          484 c          422 g          268 t
ORIGIN      
        1 gcgctggaac cgccccgccg cctccgtgga ggaatgcaac tgcatcgagg aaaagaaagc
       61 ggacgcttcc ttcgaaaacg gccaaattca tttcaccatc ctgccctatg agatcaagac
      121 cttccgcatc cgggaacagg aggtgtgacc atggactttc tgcccatgcc ccactccgtc
      181 cgcaaagggg agggactctt ccccctgcgc tatgataccc gcatcgtgct ggaaaacacg
      241 gagccttccg cgtttctcta cgcccagatg ctgcaggagg acattcatcg ctttaccgga
      301 ctgaaaccgg ccattctccg gggaacggcc cagccgggcg acgtgattct ccgggcggat
      361 gcgtcgttgg aggaaaccag ttaccggctc tccattacgc cggaaaaagt gctcgtctgc
      421 ggcggcannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnncctcaag cgctacgccg acctgctgtg ccgctacaaa
      601 atcaacgaat ggcagctgta catcgagcat acctatctct tccgcaatct gagtgaagcg
      661 tggcgggggg atacgcccct cactgccgag gagatcatgg agctggatcg ctactgccgc
      721 gcccggcaca tcgaactgat tccttctctg tccagcttcg gccacatgta caggattctc
      781 agcaccaaaa cctgctgcga cctgtgcgag ctgcccgatt cggaaaagat cccgttcagc
      841 tacacttacg ccggcgacca tcacaccctg aatgtgtcca accccaacgc cgtggatttc
      901 atcaaggggc tcatcacgga gtaccgcgct ctgttcacgt ccaaaaagtt caacatctgc
      961 tgcgacgaaa ccttcgatct gggcaagggc cgcagcaagg tgctggcaga cgaaaagggc
     1021 gagcacgccc tttacatggc ccacgtggtg gagctgtgcc gctggctgct ggcgcagggc
     1081 gtgaccccca tgttctgggg cgacatcatg tggcggcacc cggaagccta cgccgaaatt
     1141 cccgaaggcg tgatctgcct gaactggggc tatctgcccg agcagcggga aaacgaagtc
     1201 cgcgatctgg cccagatggg cgcgacccag tacgtctgtc ccggcgtatg cacctggaac
     1261 cgctggctgc ccctgacgga gaacagctac aagaacaacc gggccatgtg cggccacggg
     1321 cgcaaatacc acgccatcgg cctgctgaac acggactggg gcgactacgg ccacatctgc
     1381 cacccgtggt tctccgtgcc gggcattctc tacggcgcgg ccttcgcatg gaacgcggac
     1441 aacgtgccct tcgacgagat caatcaggcg atttccctct tcgagtacgg cgaccgggac
     1501 ggaaccttca tgaaggcctt cgtccatctg accgatcacg aaattttccc gtggttcaac
     1561 gccgtccgct ggatcgaagc cgacgacccg gagaaacgcc gggagcagct gaaggagatc
     1621 gaccccgcgc
//
LOCUS       DAWAEA010000330         1628 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_9462_length_1628_cov_1.117610, whole genome shotgun sequence.
ACCESSION   DAWAEA010000330 DAWAEA010000000
VERSION     DAWAEA010000330.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9462_length_1628_cov_1.117610"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    955..964
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1224..1360
                     /estimated_length=137
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          299 a          425 c          460 g          297 t
ORIGIN      
        1 cgggaatatc aggcggacga tctggaatcc atccgccaat gggtcaacga cgagtccacc
       61 acctgcttcc tttccacggc ctactggccg ccccagacca tggtggatac ggaagaattt
      121 ctgcagcgga tgcttcagag cagccacaac tcctttaatt tcgtcattgc agaccgggaa
      181 agcggacgct atctgggtca gctggacatg ttcaccgtgg actggcggct gcgctgcggc
      241 aagctgggca tggtgatcgc cggggcggaa aaccggggcc ggggtcttgg cacggaagcg
      301 ctgctgcttc tggaaaagca cgttttcctg acgctgggac tggagcggct ggagctggaa
      361 gtccgcatgg acaccgccgc cgcccgccgc tgctacgagc gtgccggttt ccggctggaa
      421 ggcgtgaagc gccacgcgtt tttcgcaaac ggagccttct gcgatgtggg gatcatgagc
      481 atcctccggg aggaatacgc ccggtaacgc ggttcagttc aaatgcatat tctacaccat
      541 cccttgtttt tcggctttcg ccgtttcgag ggatggcgtt ttttcggcct gaaagccgcc
      601 tacgggaggc agtattgact ttcaggccga aaatcggata aaataaagct tcggaggcgg
      661 ctagctctcc gtgatattgg aggaaacgat tccatgggaa aaattgtggt caaattcggc
      721 ggcagttccc tggcggacgc agctcagttc cgaaaagtgc gcgatattat ccgaatggat
      781 ccgcgccgct gctacgtggt gcccagcgcg cccgggcggc gttttccgga ggatgaaaaa
      841 atcaccgacc tgctctacca gacctaccgc gcccagcgaa acggcgaaaa catcgagccg
      901 atttttcgcc gggtacggga gcgctatctg tccatcgcgg aagaactggg gcttnnnnnn
      961 nnnngtggaa cagctcatcc gggaaggagc cagcgaggat ttctgcgcca gccggggcga
     1021 gtatctcaac ggtctgctgc tggcggacta tctgggcttt gcctttctgg atcccaaaga
     1081 tctggtgttt ttcaacgacg acggcacctt tgacagcgaa cgcaccaacg atgtgatggc
     1141 cgagaagctg aaagccgtgc ctcaggccgt gatccccggt ttttacggta gcatccgggg
     1201 cggcgaagtc cacacctttt cccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gggcgcgacc gtgctccacg
     1381 aggacagcgt atttcccgtc caccgggcgg gcatccccac caacatccgc aacaccaacg
     1441 ccccggagga gatcggcacc atgatcaccc acggcgcggt ggaggaacct aacccctatg
     1501 tcatcaccgg catcgcgggg cggcttggct tcagcgtcat ctctgtggaa aaggccatga
     1561 tgaacggcga ggtgggcttc ggccgcaagg tgctgcaggc ggtggaggag ttcggcctgt
     1621 cttttgag
//
LOCUS       DAWAEA010000331         1625 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_9474_length_1625_cov_2.671338, whole genome shotgun sequence.
ACCESSION   DAWAEA010000331 DAWAEA010000000
VERSION     DAWAEA010000331.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9474_length_1625_cov_2.671338"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          301 a          518 c          477 g          329 t
ORIGIN      
        1 ctccacgatg agtccctgac cgacggtcag acccatggct ttggtcacgg tgagcagatt
       61 ctccattttt ccctgctcga tgtagccggt cagcagcaac agccgtccgc cgttttccac
      121 atactgaacc agcatttcgg cctcgtcggc gctcaaatcg ctggtcggcg cgttaatcat
      181 gaccacgctg gcgtcctcgg gcacgctctc catggaaagc agactcagcg tctcggtctc
      241 cacgttgtcg cccgccagca gttcctccat gtagtcgctg agttcgcttt tgccatggcc
      301 ggtaagttgg tacaccttag gcaggttctg gctggacaca tagtgaatgg cgttggtcag
      361 cgcgttctcg ccgtcaaagc tggtggaggt atcgtactgg taggtgctgt agttcatgct
      421 gtagtcggtc acataaatgt cgctgtagct gaccagtcgg ctgcgtccgc cgccgtccac
      481 cagcacgctg ttggcataca gctggttgat gtccagatca taaacgtcca aaaacgtggg
      541 ctgctccgtg ggatccacgt tggacagatg aatgtggctg ttcagctccg catagctctg
      601 caacaggcgg gagatggtat cgtcctcgtt gccgcgcagg gccagcaggt acaggttcac
      661 atccgtatcc agactggcgg cgatgcgccg ggtctggtcg gacagggtat acagggactg
      721 cgccgtcagg ccgaacacgc cgtccacaaa gctatggagc gttcttttca cccttcccgg
      781 cgttcgtgaa ggatctggaa ccctttggcc ggaagacgga gcgtctgccc cgccaactcg
      841 ccgatgccgt agtccgcctc cagcgagcgc gtcaggcggc agacctgctc ccggggcagg
      901 gggttaaaaa caaaaatgta ccgttccgtt tcggaaaacc gggtcaccac gctgagacca
      961 taggccatct ccaccggcgg cagcgaaacg ccgcagcgtt ccagcagcca gcgaagcagc
     1021 gcgggcgcgt cctcgggggc cggagccgtg cccagataca gggcgctgcc ctttccatag
     1081 ggatgctcgg tcagcacagg ccagccgtcc cggtaagtgc cgcgaaatac gcccagcacc
     1141 tccgcgccct cggattccag tgtttcctgc cagaagtggc ttcggatggt cagttccccg
     1201 atccgcaccg gcgcgtcggt ctggccgtca aagaccggct ccatctccac cacatgcatc
     1261 ccgaaaaagt ccgtcatgcc caccgggctt ttttcgaaaa tgccgctgcc ccattcatcc
     1321 ctgctgaacg ccatggtcat ggccagcagg tggccgccct tttcggcata gtcccggcac
     1381 ttccgacgga agtcctccgg caggatgggc gcaaagggca caaggaccag ccggtagccg
     1441 tccagcggat ggtcggcgcc gatcaggtcc acattcacgc ccagcgccgc cagctgccct
     1501 accagctgct gcgcatagcc ttcgtaggta aattccttgt ggatgggctt gatgcggttg
     1561 gcccacaggc tgttgtagtc cagcaccacg gccacttcgg agaccacctt ggtgcggttc
     1621 aggat
//
LOCUS       DAWAEA010000332         1621 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_9500_length_1621_cov_1.446360, whole genome shotgun sequence.
ACCESSION   DAWAEA010000332 DAWAEA010000000
VERSION     DAWAEA010000332.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9500_length_1621_cov_1.446360"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    411..450
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    939..1011
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1381..1392
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          348 a          379 c          485 g          284 t
ORIGIN      
        1 gaatggatcg cctatgccct gcgcaaggcg ctggtctggt cgtttctggg ggagaaagtc
       61 catgtggaga tggatcgtcc catcgggacg gagcatccca agcacccggg gctttattac
      121 cccatcaatt acggctatgt tcccggcgtg ctgggcggcg acggggagga actggacgta
      181 tatgtgctgg gcgtttcgga acccgtgcag acttttgacg ggcgggtgat cgggatcgtg
      241 caccggcagg acgacaacga ggacaagctg gtcgccgcac cggacgggag gatcttcgat
      301 caggcgcaaa tcgcccagca ggtgtatttt caggaaaaat actaccacag cgaggtggag
      361 ccgctgtatc atcactcctg cggtgtgatc cccttccggc ggggcgaaaa nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gaggggagac tgagcgggaa acggtgcttc
      481 gggaggcgtg ggaggaaacc ggctgccggg cgcggctgct ggacggcttc cgggaggaga
      541 tcacctacgc catggaggac cgtaaggggc agaagcgggt cacgctggtg ctgggccgtt
      601 tggagggaga attgtccctt cgggaggaag agattgccgc cgcccgctgg atgagcgccg
      661 aagaagcgct gccccttttg cacaaaggct atcggacgat tctggacaaa gcggaggaat
      721 ttttgtcaaa acagggctga acggacaaaa ttcccctttt cacgccaaaa acccgccccg
      781 gaaacccttg tatttcctgc agtgatgtgg tatactgacc ctgcctttca ttcgaaggca
      841 ggtttctggt accatttcga cgaaggatta cagaaagaag ggttttcgaa aagagtgggt
      901 atcgcttttc ttttctgctt gaaggcaagt gacagcatnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggcaaatgc
     1021 ctgctggtat ttatcgaccg ggagggcggc atcgggctgg acgactgcga gaaatatcac
     1081 cgggcgctac agccactagt ggaggatgtg gattacgaca tcatggaggt ctccagcgcg
     1141 ggcatggatc gccccatcaa gacccggcgg gattttgaga aaaaccggga cgcgctggtg
     1201 gaagtccggc tgtttgccgc gcagaacggc tccaagctgt atcagggcct tctgaaggac
     1261 tttaccgacg aggaagtcgt gctgcagccc gcggagggcg aactgctttc cttcccccgg
     1321 aaggccgtgg ccatcgtcaa gcctgtgatc gactttgacg aggacgacct tcccgacgat
     1381 nnnnnnnnnn nnaataagaa tatcaataaa caggagtaaa agacaatgag ggttacgaaa
     1441 aagaatcagc agcagaacgg gcaccgggag atcgtggacg cagtcaaggc gctgtgctcc
     1501 gaaaaggata tcgagccgga ggttctcttc agcgcgattg aggaggccat gaaggccgcc
     1561 tacaaaaaga acgtctcccg ggacgaggac gcgcccagca acctgtccgt caccatggat
     1621 c
//
LOCUS       DAWAEA010000333         1597 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_9629_length_1597_cov_2.413748, whole genome shotgun sequence.
ACCESSION   DAWAEA010000333 DAWAEA010000000
VERSION     DAWAEA010000333.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9629_length_1597_cov_2.413748"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          262 a          511 c          488 g          336 t
ORIGIN      
        1 cctccgggcg gctgaacctg catgacctca tcagcgaccg ggtagccctg agcgacatcg
       61 gcacggcttt cctgccaggc ccccgcaacg gtaagacggt catcctgccc tgaaccccat
      121 tcgaggatag gagggcccga cgaaagtgct gggaatgttg tatctgctgt tctttctggc
      181 ggggggcgta ttcctgacaa ggatgctgct gccccgccgc aagcccgtgc tgcgggtgta
      241 tctgggcctg agtctgggcc ttttcctgat gatgtggctg ccggtgctgt gggcttacgc
      301 ggtgcggttt tcctatacgg cccacgccct agcggcaggt tcgctggccg cgctgtgcgt
      361 ccttggctgg ctctgccggg ataagaccgc ccccgcgcct atggacgagc ggcagaaaaa
      421 gcttctgctg gcgctggccg tgatggtgat cccgctgggc gcagtcagcg ggtatctgca
      481 atatacccac tccatccggc tggctgcgga cggctcctat cacgtggggc agagtaccta
      541 cggcgactta agcctgcatc tggccatctg caccagcatc atcaacgcca aatttccgct
      601 ggaaaacagc ctgatgcttg gggccaccat ggcctatccc tacctgtcgg actcggtggc
      661 gtccacgttc tacctgttcg ggatgccgct gaatacgtcg atggccttca ccggcaccct
      721 gatgatgctg ctgacctacg ccggctacgg cctgctggcc gaccagcttt gccggaggaa
      781 gggcgcgcgc tggctggcct tcttcctgct gttcctgaac ggcgggctgg gcttcttctt
      841 caccctgtcc ggccgggtag aggacggcgt gaccaccacc ttctgggata acctgcaaac
      901 ggtgatgcag ggctactaca agaccccaac caatcagccc gaccccaaca acctgcgctg
      961 gtccaatatc gtggtggatc tgctgatccc ccagcggggg ctgctgggcg gctggaccat
     1021 gctgatgccc tgcctgaatc tgctgctccc gcccctgtgc cggggcgaaa agcacgagac
     1081 ccgggcgctg gtgattctgg ggatcatggc gggcggcctg ccccttctgc acacccacag
     1141 ctatctggcg ctggtgcttt tgtcgctggg aagctgcctt tactgcgtgt tccgggacgg
     1201 cgaaaaggcg ggccgctggc agcggttccg cccatggctt ctgtacgggg ccattgccgc
     1261 agccctgtcc ctgccccagc tttggtgctt caccttcgta caggcaacgg aaagcaatca
     1321 cttcctgcgg ttccagttca actggtgcaa caaccggggc tgcagagggc tggtggatcc
     1381 ctatttcttc ttctatatca agaacgttgg ctttccctat ctgctgatcc tgctggcgct
     1441 gctgaagcgg aagcccagag agctggaagc ccccggcgcg gcggaagaag cggcggcggt
     1501 acgccagtac cggctcatcg cctgcggcgc gttcctgatc tacggcattg cggagttcat
     1561 tctctttcag cccaacgaat acgacaacaa caagctg
//
LOCUS       DAWAEA010000334         1591 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_9670_length_1591_cov_1.578125, whole genome shotgun sequence.
ACCESSION   DAWAEA010000334 DAWAEA010000000
VERSION     DAWAEA010000334.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9670_length_1591_cov_1.578125"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    662..706
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          320 a          453 c          442 g          331 t
ORIGIN      
        1 tcctgccctt catggtattc cccatctaca ccagcctgag caagatggac cccaagctgg
       61 gcgaggccgc catggatctg ggctgcaatg aggccaagac cctgctgaag gtgaccatgc
      121 cgctgagcat ccccggcgtg atcagcggga tcacgatggt gttcatgccg tctgtgacca
      181 cgttcttcat tccccgggtg ctgggcggtg gctccacgca gatgttcggc gacctgatcg
      241 agaccacgtt cctgaccgcc ggcgactgga atctgggcag tgccctttcc ctgatcatga
      301 tggtgctgat cctgatctcc ctgtccatcc tgcgcaaggc ggatcctaac ggggaaggag
      361 gcggcatcgc atgaagaact ttttcaagaa gttttacctg gggatcattc tggtggcaag
      421 agccgggcca agtgggaggg cttctccttc cgctggtacg gcgaactgtt ccagaacgcg
      481 tccatcatga acgccctgtg ggtgacggtg tctatcgcgg tgatcgccac ggtgatcgcc
      541 acggtgctgg gcacgctggc cgccatcggc atttacagca tgaagaagcg gcctcaagcc
      601 atcatgatga ccatcaccaa tttgcccaac accatgccgg acatcgtgac cggtatctct
      661 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngagg cggcgctgga
      721 tctgggcgct acgcccggct acgcgctgac tcatgtgatc gtgcccgagt gccgtcaggg
      781 catcgtcacc ggcgcgctgc tggccttcac cctgtcgctg gatgatttca ccatcagcta
      841 tttcaccacc agtccgttgg tgcagaacct gtctacgctg atctattcgg ccaccaagct
      901 gggcatcagc cccgtgtact atgccctttc ctccctgatg ttcgtggttt tgctgattct
      961 gctgttgatc gtcaaccgca ggaccgccga gcccaatggc ttaggaaggg ccggacggct
     1021 tttccttaga cagagattgc cctgtcaaaa aagatgatag gaggaatccc acatgaaaaa
     1081 aatggttgcc tttctgctct gtatcgccct gctgcttccc gcctgtgctc tggcgcagga
     1141 agtggtgaac gtgttcaact gggaagacta tatcgacgag tccgtgctgc aggacttcga
     1201 agccgaaacc ggcattcacg tcaactacat gcgctttacc accaacgagg acatgctggt
     1261 gcaggtggaa gccaatcctg gcgctttcga cgtggtgttc ccgtccgaat actgcgtgga
     1321 gcgtctggcc aacaagggcc ttttggccga gatcaactat gacaacattc ccaatttcca
     1381 gaacatcaag gaaagcctga aaaaccccag ctacgacgag ggcaacgccc attccgtgcc
     1441 ctatcagtgg ggcgctctgg gcatcctgta caacaccgag atggtggacg cggaggacgt
     1501 gaagacctgg aacgtgctct ggaacgagaa gtacaaggga caggtgctga tgatgaacag
     1561 catccgcgac gccatgggca tcgcgctgaa g
//
LOCUS       DAWAEA010000335         1584 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_9715_length_1584_cov_1.680183, whole genome shotgun sequence.
ACCESSION   DAWAEA010000335 DAWAEA010000000
VERSION     DAWAEA010000335.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9715_length_1584_cov_1.680183"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1233..1242
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          366 a          438 c          374 g          396 t
ORIGIN      
        1 catctgccgc acctcgctca ccgtatcggc acttagaatt atgcggtatt gcctattctt
       61 tattacttat tctttttttc gcgcccaact cctgacggtc tctctgccta attttctttc
      121 gtcaaacttg ctattttccg ttttctccaa actttcacac acttgtttgt aaataatcat
      181 gaatttatca caaatgtatt gacgatgggg caggtttggc gtatgataca gatgttgaaa
      241 caaatattcc ggtaaacatg aaatttgaga aacaatagtt cgaagccgtc ttcgtgtggc
      301 agccagccgc ggggtatttg ttgccgggta accggggcgc aaggcagtat tggccttggt
      361 ttacccattt ttgtggaaca cgccgctttc gcttgcaacc ctgatagcgg cattataaaa
      421 aggaaaacca tattaaggag gataccccat gaaaaagtca gtcagccgta ccttggccat
      481 ggctttggcc gcctgcatgg cagcaggcac tttggccgcc tgtggaagca gcggttcttc
      541 cgcggtcgct tccagcgaag ctgagtctac cgcagattcc actgccgctt ccaccgaatc
      601 cggcaccccg gattactcca atgaggaccc catcaacatc cgaatttatc tgtcggataa
      661 tgctaccctt ccttataaag aagactggct cacctttacc gaagtgcaga agcgctgcaa
      721 cgtcaacctg gacgttgagg ccattcctat tgcggactac tctaccaagg tttctctggc
      781 actgaatacc ggcgagaacg ttcccgacgt catcctgtac cagtccacca ccggtgagaa
      841 tgcttctttg gctctgaacg gcgctctggt tcccatcagc gactacagcg actggacccc
      901 caacttcaac gcccgcgttg aggaaatgga cctgcaggac gatgtggata tgctgaacct
      961 gcaggacggc aagcgctact atatgccgtc tttgaccgat aagccgttct acgacggcgg
     1021 cctgatcctg cgtgaggatt atctggaatc caagggtctg gaagccccca agacttttga
     1081 tgacttgtac gagatcctga aggcttacaa agctgattat cccgattctt acccgctcac
     1141 cattctggct ggtccccgcg ttctgttccg tatgacgatg ccttccttcg gcatcagcgt
     1201 gggcaaaaac agcgccgatg gctcttatgt tcnnnnnnnn nngagctatg attacgacaa
     1261 caaggatttc tttgctggcg caattgatga taagtgcaaa gaatacttcg cattcctgaa
     1321 caagctctac tccgaaggcc tgcttgaccc cgaaatggct gaccccattg atggtgataa
     1381 gtggagccag aagttggcta ccggttcttc catggccacc tacgcttact atgaccagat
     1441 cggcggcgtg gaagctgcca gcgagatcga cggctttaaa ctgcagatgt atgcaccgct
     1501 ggaaggcccc gcaggtgccc accaccagcc caagtcccgc accggttccg gcatcatgtt
     1561 ccccaaaaag actgctgaac gcga
//
LOCUS       DAWAEA010000336         1582 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_9727_length_1582_cov_1.292076, whole genome shotgun sequence.
ACCESSION   DAWAEA010000336 DAWAEA010000000
VERSION     DAWAEA010000336.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9727_length_1582_cov_1.292076"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    264..409
                     /estimated_length=146
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          269 a          459 c          440 g          268 t
ORIGIN      
        1 cacgacatcg ctccctacgg tctgcgcaac ctgctggcca ccgttctccg ggacgagctg
       61 accgcgctgg gcttcgatcc tgcgcccgcc agcatccggg tgaataccgt cagcgacgcg
      121 cgtctgtccg gcgaaggcaa gcgcatggac accctgtacc tgacggtgca gatcagcgga
      181 cgcacccggc aggacgcggc cgttccggcg gtcgattccg atcaggagga agtcagtctg
      241 gtgctggacg gcagcgccga aacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gccagcgcgg
      421 cgcggaatgg atggcgggac gctatcagaa aacctatcag gaaattctcg ctttctacta
      481 tccgggcatg tccctgatgc gctacgactt tgccgaatca gctctgcccg ccgccccgga
      541 agcgctggca gctacgcccg gccccgcgcc tacgcccacg ccccggccca ccctgatgcc
      601 ggtgagcgag cagcccaccg acggccagtg gatcgcgctg gtaacgggca tcgacgacga
      661 cagctccctg aacctgcgtt ccctgcccga tctgagcggt gatatcgtca tgcggcttta
      721 caaaaatcag cggctgctgg tgctggaacg ctgcgaggag gaaggctggg tcaaggtgcg
      781 caccgacgcc gccgagggct acgtcatgga aagctacctg acggcggaat aacaggacca
      841 tcccttctat tcaacgatcc ctgctgccaa agggcgcggt ttccttcggc ggcagggata
      901 gcttttttcg tgtttttgtc gtatactgtc gaaggacccc ttgcatttca gccatggctg
      961 acgatgccga aaggaggcga cccgcatgaa tccctctccc cgtccgggcg ttcagccgcc
     1021 cgagtccgcc tatggcggcc cctacggctc cccggagcct actcccccgc cccatcggag
     1081 gcgtagtcgc cccggccggt ctcgccgctt tgtgcgcggc tatctgatgc tggtgggcgc
     1141 gctgaccacg ctgtacgtgc tggtgcagct gctggtgcgg ctctttattg aaataggcaa
     1201 atggatgccc cctgcctgag aaaggtggaa cccatgaaaa aattcattcg aaagctgttt
     1261 accgtcattt tgctggcggc gctggtcttt acgggcattc gcttcgggcc tgcgctctac
     1321 gcccgaattt tcggcggcgg caacgtccag tggatcagcg aacgtttcag cgagaccctg
     1381 aaggaaaaga atcacctggt ggtttatgag gtagagacta ccgggcagga gaccgtgacg
     1441 caggatgcgt ggcttttcgg cacggtgcag aaagtggtcg taccctacac cttccagatg
     1501 agctacaccg tcgagcgggg ccaagacggg aactggctga tcgtcgtgac caacgcccaa
     1561 acgccctcag gcggcgagac cc
//
LOCUS       DAWAEA010000337         1558 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_9880_length_1558_cov_2.129075, whole genome shotgun sequence.
ACCESSION   DAWAEA010000337 DAWAEA010000000
VERSION     DAWAEA010000337.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9880_length_1558_cov_2.129075"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          285 a          430 c          507 g          336 t
ORIGIN      
        1 atgatctggg cggctatctg cgccgccggg ccgcgcggct tctgcccgcc tactggcctg
       61 tctgccttgt ctggctgtgc acgatgatcc cggcgtttgg cttttcgcct gtacagacct
      121 tgcagaccat tgcgggcaac gtgctgatgg tgggctatct ggcgggcgcg ccgctgaata
      181 tcaactggta cccctcctgc ctgctgctga cgctgctgct ggccccggct gtttacgcct
      241 gcctgaaaaa cagccgaaag cccctctgga cgctgctttt gctgctggcg gcggcgttcg
      301 gcgtgggaat cagctatttc ggcaacgcac agcttctgct gttctcccgg agcatgggtc
      361 tggccatgat ggaacgcggc aaactgcaaa accctctgcg gggcgcggcg tggccggttt
      421 ttctgaccgt ctgctttgcg gcgggcttgg gcgttttgtg cttcggtgag ttcaaacacc
      481 cggaaatgct ggaatacgag ggtctgtact ggtactcctt cttcctgatg atcccgcccg
      541 tctgtctggg gctgggccgg ctattccggc tgggcgggga gaaaaacgcc gctttcgcgc
      601 ctttgcgggc gctgggcagc gcttcctttg aaattttcct gttcaacgtc tggatcgagg
      661 tgctggtgaa aaaggtctgg tgctgggaac cctgctgggg ctacctgtgg atgggattgc
      721 tgagcatccc actgggcctt ggctggcatt gggtcgtggg cagaatgacg cggcgaatgg
      781 agggccgaaa gactcatgca gtatcgtaaa gaccgctatg gcaatcccat ttccgcgctg
      841 ggcttcggct gtatgcgctt tacccgcaag ggcggagcct tcgatatcga caaggcggaa
      901 aaagaggtgc tccgcgctat cgaactgggc gtgaactatt tcgacacggc ctatctgtac
      961 gtgggcaacg aggcctgtct gggcgaaatc ctatcccgga accacctgcg ggaaaaggtg
     1021 aacattgcca ccaagctgcc ccagcacaag atcggcagcg ccgaggggat cgaaagctgc
     1081 tttcaggagg agctgcgccg gctgaaaacg gattatatcg actattatct gatgcatatg
     1141 ctgaccgata tcgcttcgtg ggagcggctg gaaaagctgg gcattcggga ctggatcgcc
     1201 cggaagaagc gggaaggcgc gattcggcag atcggctttt cctttcacgg caatacggaa
     1261 atgttcctga aaattttgaa cgcctacgat tgggatttct gccagatcca atacaattat
     1321 atggacgaaa acgcgcaggc gggccgaaag gggctgcggg ctgcggcgga gaagggcatt
     1381 cccgttatca tcatggagcc gctgcggggc ggacggctgg tggagctgct gcccgaaaag
     1441 gccaaagccc tgatggagcg ccatacgccc cggcgcagcc cggcggagtg ggccctgcgc
     1501 tggctgtgga accagccgga ggtgacctgc gtcctttccg gcatgaacag cctcgata
//
LOCUS       DAWAEA010000338         1557 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_9891_length_1557_cov_1.065912, whole genome shotgun sequence.
ACCESSION   DAWAEA010000338 DAWAEA010000000
VERSION     DAWAEA010000338.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9891_length_1557_cov_1.065912"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1110..1289
                     /estimated_length=180
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          283 a          412 c          362 g          320 t
ORIGIN      
        1 aatctccgtt tctgttgttt tggtatactt cgccaccttt tccggggtgg aaaggtcgaa
       61 gatcggcgtc cacgtgtccg gcatccgcac ggaatagtac gcgctgattt ggcagcctcg
      121 tatggctttt tgagccatat agcccgtcgc gccgggggtc gttccgtaag tggcgacaaa
      181 gtacaggtat tccgtctgaa aagaggcttt ccccaaaaac tccctgacga tacttggcag
      241 gctccaatca taggtgggcg acacgatccc gatggtctcg tcggcaaaaa cataacgatt
      301 ttcccggatg cagtccacga tcgacagcgt cggctgtccg tcggcctgtg caagccgcgc
      361 cgcaacgtac tggcagtttc ctgtggcgga aaagtataaa atcattggtt ttcctcccca
      421 atatccactt ttgggcattt ctttcatgcc aacgcgcatc aaaagtcgga aagggatttt
      481 gccgcaaaac aagcatccat ttttccaaat tttcacacaa aaagtgtctt ttttcaagtc
      541 ttgttttttt cacccatgtt ccggtatgct ggaagcacct tttcgaagcg gacaggggta
      601 acgaaaaacg gcggggattt ctccccgccg cttttgataa tcgcatgttt ttcatgcgcc
      661 gcaaattttc actgctgttc cagcacccag tcggcggctt tccgcaggga atccaccacg
      721 ggcacgcccg cttccagcag agtctcccgg ctttgatgac cgcgggctac cagaatgcac
      781 cgggtcccca atgcacgggc cacctccgcg tcatgcaggc tgtcgccgat catgacggtg
      841 gcctccgccg gaacgccgca gctttgcaga tattcccggc caatggcctc cttgctgccc
      901 gcgtagatat cgccccggcc cagaatcgca tcgaaccgac tgttcagccc gtacagcgcc
      961 agctggcggc gcagcatggt ctgctcgctg gcggaaagca ccgtctgccg cagtccggcc
     1021 cggtggaagc gttccaccgt ttccaccgcg tcttcgtgca gggggatggt gtccatgcag
     1081 cgctcgtatt cggccatcca cgcatgggcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng tcatcgaaaa tctcatccgt cacgcccgcc
     1321 cgctggtaat acagccgcac gggaaaggtg aactgccggt gatattcctc cacgccgctc
     1381 agcgtcggca gatgaaaggc cggaaagatg ttgtttcgca cctgaatgct gtattccaga
     1441 tcatccagaa gggtgccgtt ccagtcccat aaaacctgct ggatcttcat catcaatagg
     1501 ccttggcgaa atagatcagc ttcttggcgg gcttgccgca gcagacgcag tgatcgt
//
LOCUS       DAWAEA010000339         1550 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_9931_length_1550_cov_1.787960, whole genome shotgun sequence.
ACCESSION   DAWAEA010000339 DAWAEA010000000
VERSION     DAWAEA010000339.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9931_length_1550_cov_1.787960"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          298 a          472 c          442 g          338 t
ORIGIN      
        1 caaccatttc cgccacttca ggattgttca gcgtctcgct gacccggtcg aacacccgct
       61 ccttgttgtg atcccagatc agcaggcaga cgtcctccag atgatggcgc tttagggctg
      121 gatagagatg gtgctgaatg aactcgcgct cctgagcggc gttcatcagg caggaatccc
      181 acgtctgcac ggccttgggt tcgttctggg aggatagcat cttcacatct acgcccgcct
      241 gctgataggc ttcgatgaag cggcagatgt attcggccca caggctgtaa tattcgggct
      301 tcagttcgcc gccatgcagc cgggaatgat tggtcttcat ccattccggg ggactccacg
      361 gggtcagcat catgccgatg ggccgtccgg cttcctcctg cgccatcttc accagcggaa
      421 ggacgtactt ctccgaacgc gccgggtcaa aatccttcaa ttcaaagtcg tccggggtat
      481 tatcggagca atagatgccc gcggagaaat cgcagctgtc cacggaggtc cggcccagcg
      541 tgtaggcgtt gccctccgtg gagaaatagt cgtgagccag cttgcgctga agctcctccg
      601 gcattttggc gaacacatag ccggacgcgt cggtgaacgc gccgccaaag ccctgaatgg
      661 tctggtactg cacctcgggg tacagattga tcagccggtt ctcctgctcg gtcagcgtct
      721 cgtcccagtg aagctccgcg acgctttttt ccttttggcc gtccagataa cgggtcgtta
      781 ctttcttcat cttcatcctg tcctttcggc aatggtgcat ttcttctcaa agatgacctt
      841 cggtttgggc gaatgtatcg gcagtctgat cggcaggcac ccatgcgtcg tcgaaatgga
      901 tgatgacgcc ggtatcggcc agcaccttct gaattgcgga cacaggcgcg tcataaacgg
      961 gcttttgctc gtccaatgcc agcgccgcgg caatcccggc cgcctgaccg gtcagcacgg
     1021 cgggcgggat cacccgcagc acgtcccagc cccagctgga cgcggaagcg ctgcggccgc
     1081 aggtcagcag gttatcgaag ccatgcttga ccaaggcgcc gaagggcacc tcgaacagcc
     1141 ggtcccgatg atcgaaatcg caaatggctc caatcgaggt ttcctgatgg cggtacagat
     1201 catccatggt caaggtcgca tcgccgtcga tatggcgagt ggtgcgcagc tgggcctcgg
     1261 cgggcaggta ctgcacgtcc cgctccattc ggggctgatc cttcaccttc tcaaacagct
     1321 tgagctgatt ctgaatcaga taatcgctga caacctccac ggacgcgccg gggaatttgg
     1381 gcatatccgc cgggtgatac tgcccgtaca gggacgcatt gccgccgtga tagctcacac
     1441 aggccagacc cacatcgttt ttctccaccg cctgtttgca gtgggccaaa tcggtgccat
     1501 aggccaccat ggtaaagaaa ttttcgccgt ccaccgtggg gactcctgcc
//
LOCUS       DAWAEA010000340         1541 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_9986_length_1541_cov_1.925303, whole genome shotgun sequence.
ACCESSION   DAWAEA010000340 DAWAEA010000000
VERSION     DAWAEA010000340.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9986_length_1541_cov_1.925303"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          306 a          417 c          533 g          285 t
ORIGIN      
        1 atcctgcaca atggacaggt gcgccggggc gcggcggtcg cctttgcgct ggcggcttcc
       61 atggcgctgg tgggcgcttt cgccacaggg gagcgggcct atgtcctccg tgtgccggag
      121 acccagacgg ccatcgccgc ccattccgtc ctgacggagc aggaggccgt ggaggcggcg
      181 cagcgcttct ggcggcgccc tgacgtgagc tggaacgtct ccggactggt atggtcggcc
      241 agcgaatggg gcagggaaga atggctggtg atggccgcgc cgaaagacga tgcgcgaacc
      301 gttcgtatca cgttggaaaa agagacgggg tatatcacgt cctttgaaaa tcccttctgc
      361 atggccgaaa cctcctatgc taccatccag cctttgtcca ctgcggaggt cgatcaggtg
      421 ggcatgggcg cgctggcttt tgccgacgcg gtttacccgg gctcgtccaa tcggatcgag
      481 ggcatagaca attacggcga aggaacgcac ggggacgacc ggatcatttc gttagccggc
      541 gttgcggaca cgggcatctt ccgcttcgac atccagaccg ccccggaatg gaaaattgtt
      601 tattaccgta tgccttctga gaacgccgac ggggatgacg gcggggaagg caacggctga
      661 ccggcattca aaaggaggaa tatcagatga aacgacggct ttcgatctgg ctggccctgt
      721 gtctgctctg cctgtacggg acggcgttgg gagaagaaac cgtggtggag ttcgcaaagg
      781 cgcgcgggat gaatatcatg gagctgactg ccgggttgac ggaaatctac ggctacacgc
      841 aggaggaggc cgaccaattc ctgtgcagcg ttaccgaaac ggaaagtacg ctggaatatg
      901 tgctttaccc ggcagagcat ccgaactggc gctataccca cgtattgaac cgaaaaaacg
      961 gcgcgtgtga ggacgatctg tcgcccttca gcggtactaa tccgccgggc tatgagggct
     1021 ggctgcgggc gatcctgcag cgggcggcgg aagaaagctg gtttgaaaac tgggatgaag
     1081 cggccatgac ggcgctgtac gatcagatga cccagtggga gtttcgccgc tcgtgcaggg
     1141 gctgttcgtt tccaccttcg gggatgagtt gagctggacg gagcccgtcc gggaatggcg
     1201 ggacgaggtg ctggcgcagc acggcctgaa ccgggaaacg gtcagtaccg cgccggttca
     1261 gggcgttcag cgctggcagg cggagggacg catcaccggc aaaatgacgg tcgtggagtt
     1321 cgcaggccaa ctgccggaag aacttcaaag cctttatgta aatgaacccc atctggccgg
     1381 ttggagctgc ctgtcaggcg ccatgcgtct gccggagacc gcaggccgca gcgccctgat
     1441 cgcctttggc cggggcgagg aacggctgct ggtcatggct atggtattag aaggcgaact
     1501 gcacctgctg cctgtggggg agcggacgct tcttccggga c
//
LOCUS       DAWAEA010000341         1535 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_10039_length_1535_cov_1.577027, whole genome shotgun sequence.
ACCESSION   DAWAEA010000341 DAWAEA010000000
VERSION     DAWAEA010000341.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10039_length_1535_cov_1.577027"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          280 a          486 c          438 g          331 t
ORIGIN      
        1 cggttcacca gctcgcccag gctgcggaag gagtctacct gatcctcact gatcaggccg
       61 tcggtgtccg ggatgcagcc gatgagcagg ttggcgttcc ggcccacgct ggtcaggtac
      121 cgctggaaca gatactcggc gctgtaggtc ttgtccgcgt ccatggcatg atacatccag
      181 ccgcccttat aggcgtacat cacgtcccgc accggcatgt ccacctccac gggagcccag
      241 acagggtcca tggggtcgcc gggagagtcg ggggtcacat tgtcccgctc cggctccgtc
      301 tgatagtgag cgcaggacca gcactccggc acggccacgc cctgttcgct gccgctccac
      361 cgcagcacgt tcgtgatgcc cgggcagccg ccgaagcaga tcaggttggg ggcgattcgc
      421 ctgagcaggc cgggaatatc cgggccgccc ttttcgggcc ggagaatgcc gccgtcaaac
      481 cagatctcaa acagggggcc gtagcccgtg atcagttctt ccagctgccg cagcagcagg
      541 tcgttcagct ttttccgatt cagcgtcccg tcctcccggg tgcaaaggtc gctcagatag
      601 cgtccgccca gactgtaata caggcccggc ttgatgccgt acttatggca ggactgcaca
      661 aattccccca ctacgtcccg gggaacggcg gaattgccca catgataatc atgcaccttg
      721 gtaggccaga ggcagaagcc cgttccgtgc ttggccgtca gcagggcgta ccgcgccccc
      781 gcggccttgg ccgtgcgcag ccattgatcc gtgtccagct ttttgggatt ccagcactga
      841 atatccggga tggggtcgcc gaatttccat tccggtgcaa agacctccat atcaaaatgg
      901 aaaaagaccc ccagtccgct ctcgtgccat tccagcattt ccgccgtcgg gtttgccagc
      961 gtcatcgtgt ttgtcctcct gtttgaatta ggattcagct tctcttcttc taaaaataga
     1021 aagaagccca gccgaaaaag ttccggctag gcattcgata cggatcgtca atttccctgt
     1081 ttggtgttca gcacttcctg ccagagagtg ttgtacaccg tcagccaggt cgcgtccaga
     1141 tcgttgaaat attcgcagtt ggctacagtc tcgtcggagg ggttcatgac gggattggaa
     1201 gcgtagtcct cgcccatcag ctcgatggcc gcgtcgttgg ggcagcagta gccgatggct
     1261 tcgcagttgc gcacggccac ttcagggctg cacagatagt tgatgaactt ttcggcgttc
     1321 tccttgttct tggccgccgc agggatcact gcgcagtcca cccacacgtt gctgccctcg
     1381 tccggcacgg agtaggccag cttgtcgttg agctcgatgg cgtattccgc atcgccggag
     1441 tagaccaccg ccagcgcagc ctccccggcc accatcttat ccttgaactc gtccagaccg
     1501 taggccttca ccatgccgga ctgcttctgc tggat
//
LOCUS       DAWAEA010000342         1531 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_10069_length_1531_cov_1.206640, whole genome shotgun sequence.
ACCESSION   DAWAEA010000342 DAWAEA010000000
VERSION     DAWAEA010000342.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10069_length_1531_cov_1.206640"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    613..622
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1076..1213
                     /estimated_length=138
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          240 a          402 c          437 g          304 t
ORIGIN      
        1 gtatcaaggt catgtccgac gaggaaaaaa tcgcgctggt ggatcgcctg tcgaaatacg
       61 gccgggtata tatttcctcc gagggccccg tgccagacga attgaaggac aagctgctga
      121 aaacgcccta ccatctgatc caccacgtgc tggccttcgc ccagctggta ttcagcgaag
      181 gggccaccat tgccagcgag gcggctgtgc tgggcgttca tgccctgtac gtcaacacca
      241 ttcagagcgg ctccacccgg gagcagagcg agcgttacgg cctgttgtac tgcttccacg
      301 acccggacga gcgtttccgt caggcggcgg acaaggccgt ggagctgctg gaaacgcccc
      361 atctggtgga aatgggtcag gccaagcgac gcaagctgct ggaagaaaga gtggacatca
      421 acggctactt catgcaggaa atggaccggc tggccaaagt ggggaggaaa ggcaaatgac
      481 gtggctggat ctgggcaacg gctggcggaa tctattcctg ttgtcggcct ttctgcaggc
      541 cgtttcgctg gggacgctgt ttgcagcccg ccgctggttt tccaaaggga gccgtctggc
      601 ctgttttctg acnnnnnnnn nngatcccgg cgcttttccg gcggtgactg gcttttgtcc
      661 gccgtctttg ccattttgac aaacccgcgc tgatcgcgct gtgtttcgcc ctgtgtatgg
      721 tcattttgct ggccccggtg tgcgtccgct tcctgtccag catgaactgc gcggaagcca
      781 gcgacgcggg cgaatacctt gcgctggcgc agcgctactg cactgaccga aacgttagcg
      841 aactgctgga aaaaaacgag accgaagggc atttccgcgg caacagccat tttccctcgc
      901 tggagctgta catgagctac ggactgttcc atacgggcgg cgacagcttc ggctaccctt
      961 acgataaacc cgcctttact gccattgggt tactgctgtt ctatgtagcg gcggcctacg
     1021 gcgcgctgct tttgaccttc tgccgggaaa agaagggctg cgcgctgctg ggcgtnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnctcagtg tattggcctt tgcgggactt cagccccgca gccgcagcgt
     1261 aaaagcctat ctcggcaagc ttctgttcgc ctttgccctg tgctttacgg tgatgagtac
     1321 ccacgtggta tgcttcgtgg tgctgccctt cgtggtgatc gcctgggtga tctggcgctg
     1381 gctggaatcc ctgatgctgc gccggggcgg cgcgggccga acccttctgt ctgccgtagg
     1441 cgtggcgctg gcaggcgcgg cgggcaccgt taccgcctat ctgggcaacc tgtggtgctt
     1501 ccgccagtgg ggggaaatgt ccccctggcg g
//
LOCUS       DAWAEA010000343         1519 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_10142_length_1519_cov_1.461066, whole genome shotgun sequence.
ACCESSION   DAWAEA010000343 DAWAEA010000000
VERSION     DAWAEA010000343.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10142_length_1519_cov_1.461066"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          332 a          446 c          472 g          269 t
ORIGIN      
        1 cgtcaaagac gcgcagaaaa gggtgcaggg catagccccg cctcgtggat gcggccgtcg
       61 tcttgcagaa gaatgcggtt gccggtaagg ggctcgccgt cgagcgtggg ggcggagcag
      121 gttttggaaa tgtggaaacg gtaggtggcg ctgtaatagc gataggtgat gtgaatggaa
      181 tcccatgctt cgggaaggac ggggcgaaag tagagaacgc tgccttcctt gtggaaccca
      241 agcagttctt ccaataagat gaactggtac catgctgcgc tggcggtgta ccaagtccgc
      301 ccgccgcgcc ccagctggga ggggtgcgcg gaaacatctc cggccatcac gtatggctcc
      361 gcccggtatt gatccgccgc ttggcggtca aagccgcgat ataccggcag aagccgcagg
      421 gccagctccc acgcacggct cgactgaccc aagcggccat aagcgcccat ggcccaacag
      481 gcggcgctgg cggattgatc gccgttttct cgattacccg gtatgaaagt gggccggttt
      541 tcctcttcga agggcggcgt aaagagcttg agcagccccg cgtccggttc gtagagcagc
      601 tgccagacac tctccatggc aagctgacag cgctccttgg aaagaccgca gaaaacagcc
      661 cagacttggg gtcgcaggtc aatgcggcat tcgcggttgg aagacgcgcc gatcttctcg
      721 ctgccacggt cataggctcg cagataccac gtcccgtccc aaccatgccg ttccagagtc
      781 gtcaataact cgtcgcggcg ctcggagaga cggtgggcac ttttttcatc tgcataaggg
      841 agaaaacagc ggatgacctc gcataggaac atacccagcc agatgctttc cgtattcgcc
      901 gcatccgtac cgtcgcttaa caaaggaaaa ccatgttcgc ccagccgtat ctggtcgatg
      961 gcccggagac agtgttgcca taagggttcg atcatttcgg aaacttctgg cgtgaaacaa
     1021 tgctcctgct cgccttcttc cggcggttcg tcccgcagat agggaacgct ctcctgcaga
     1081 atggccgtgt cgccggtggc tctgacgtag gcggcggtga caaaaggcaa aaacagcgga
     1141 gcatccgaag cgggaatacg cacgccgcag cggtgcggat gccaccagat ttgtacgtcg
     1201 cctccctcaa attgatgggc tgcgcagcga agcagcagtt cccgcacaac ctccgggctg
     1261 gtgtaaagga gagcaagcgc gtcctgcaac tgatcctgaa aaacacaggc cccgctggac
     1321 ggttcgaagc cagctcgcat caaaagccgg gaggcaatca cctgataagg tagccagcgg
     1381 ttcagcatca ggcacagcgc ttcgtccggc agatcgaaac gcagcccgcc taggcggcgc
     1441 tcccagcgtt ggcggcagtg gtgcagacgc agggtagcgc catccgcccg aaaggcggca
     1501 acggctgctt ccaaacggg
//
LOCUS       DAWAEA010000344         1517 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_10157_length_1517_cov_1.410397, whole genome shotgun sequence.
ACCESSION   DAWAEA010000344 DAWAEA010000000
VERSION     DAWAEA010000344.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10157_length_1517_cov_1.410397"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1032..1041
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          310 a          444 c          425 g          328 t
ORIGIN      
        1 tgaaaaaagc catcgtagag cggctgaaaa agcggctccg ggtggatctg ctgcctgaaa
       61 ccggcccccg tgttcccatt gaaatcgcgc tgtaccggga tcaggcccgg ctgactatcg
      121 acatgagcgg cgacgcgctg aaccgccgcg gctaccgcac ctggaacggc gaagcgcctc
      181 tgcgggagac gctggcggcg gcgcttctgg acgtgactcc gtggcgtacc cggatgccat
      241 caaggccgct gcccgtcccg cggggatcga tcgaagcttc gtctgtgagg agtttccctt
      301 catgaaagac gtgcccatgg cagcgattcg tcaggccgaa cgggacaaga taccgactga
      361 gcgctctttt tcgatcgcgg gcagcgatat tgacccggag gcactggaac tgtgccgccg
      421 ccatattcat caggcgggct ttgacgggca gatcagcgtc cggcagcagg acctgcgcac
      481 actgcaagtt cccgacgagc caggggtatt tctctgcaac ccgccctacg gcgaacggct
      541 gggcgacaag cgggaagcgg aggctcttta ccggcaaatg ggccttttgc tgtagcgtca
      601 cccaggctgg aagctttgcg ccatcacgtc tgactttgcc tttgagcgct gcttcggtcg
      661 ccgggccacg cacaaacgtc gcctgtacaa cggtcggctg gaatgcgagt tttatatttt
      721 tgaataacgc aaaaacgccg cgcagcaatg acttgcagcg cggcgtctct tttggcttag
      781 ggaatctgaa attctaacgt ggttaactgc ccattccatt ccgaaccgtc gcagcgataa
      841 tactgccacc ggctggttcc gtctgtcaat gtataacaaa tggcaaagac ctgtccttcg
      901 tcgatcggca tggggtaatt gtttgcagga agaatgtcac cggtcaactt ggcggttttg
      961 aaatcgtcat aggcttcttc cagccggtaa aatttccgag aaaggtactt tccttccaaa
     1021 acgccggaaa gnnnnnnnnn nctcgccaaa ataaccggcc tgatattcct taccgccgat
     1081 ggtgaaaagc gctgctcgaa tcggttcgcc cgtcagccct tgggtggaca aatcggtgct
     1141 gtgcagccgt tcaccgtagg tctggcgaag ctgattggcc atgcagggca cctgccctga
     1201 ccagtccagc gcctccagcc cggtatgagc cgctccatag atccccatga tgctttcacc
     1261 attcagcagg gcatattccc gcagaaaatt ttcgaccatg gtattttccc gatacacatc
     1321 gtccttggta cggtaatact gctgaccctg ttcgatattc tgccgggcca actgcgcctc
     1381 ctcggaatcc gcaaggtcct gacgggtcaa atcgttcaga tagcgcctcc cggtgctctg
     1441 gtactggtgg cccacgtccg tcccatgaaa gaccgtttcc gggcaggttt ccttgatctg
     1501 ccggtaaaag cggctga
//
LOCUS       DAWAEA010000345         1513 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_10180_length_1513_cov_2.291495, whole genome shotgun sequence.
ACCESSION   DAWAEA010000345 DAWAEA010000000
VERSION     DAWAEA010000345.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10180_length_1513_cov_2.291495"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          295 a          464 c          469 g          285 t
ORIGIN      
        1 cccagaaatg aacgccgttt tccaccattt ccacccggtc gggaacggtg ccgtacaaca
       61 ggccggtctg ctgggtcagc ccttccagct cccgcacggg aacgtcgttg cgctcataaa
      121 taccggcggg gtggacttcc tccgccagcg cgtccacgat gtcctgcttg aactgctcca
      181 tgccgaggca cagcacctgc agcactacga tatcgccgaa tttgtcggcg atgacggcag
      241 gcagaccgtc gctttccgca aacagcagac ggcagctgtt caggtcagca aaggtacggc
      301 ggtagtcgat ggccgttttc acctgccggt ggatgaaagc gctgtcgatg ggttcttctt
      361 tccggctcag gatgcgcagg ctgatctggg agcgggggtt gtagaccgcc atggccagaa
      421 accggccccg gctggacacg acgcgcacta cgtcgccgtc ctgacagggg ccttcgaccc
      481 ggtcgatatc gctgcggaac acccacgggt gtccgctgta gacccgctgt tccttcttgg
      541 ggtgcaaaac cactgtaggc atggaatgtc tccttctgtt tgcgttactt catcacacgc
      601 cgcaggaaat cggcggcgaa ggcgggccag tcggcgttat ccggcacgat ctggcctggg
      661 tcgttcacgt tggcggtctc atggttgcac agggacaggc cgtggccgcc aaaggggtaa
      721 atgtgaattt ccgtgtccac cttgagggaa cggagctttt cggccaaaat cagcgagttc
      781 tccacgggaa cgcagccgtc cacgaaggtg tgccacagga aggtgggcgg acagttctcc
      841 gtcgcccagt tttccagcga atactgagga tgctggcttt cgtcctttgt gccggacagc
      901 gctacaaagg agccgcggtg ggcgaagggg ccgccgctga ccacaggata gctgagaacc
      961 atggcgttgg gccgcacgtc ctcaggggtc aggcccattt cctgccacag ctccgcccgc
     1021 tgccacagta cgcccagact gcccgctgcg tgtccgccgg cggagaagcc catcacggcg
     1081 attcggttgg ggtcggtgtg ggtctccccc gcatgagcgc gcacccacgc caccgccgcg
     1141 gccacatcct gctgaggcaa gggatagcgg ttgggcgcta cgcggtacca caccacaaag
     1201 cagttgaagc cctccggcag gaaacgcagc gcgatcggct cggcctcccg ctcacttaaa
     1261 aactcgtagc cgccgccggg gcagatcacg atcgccgggc ggcgtaccgc cagatcgatc
     1321 tctttagata ccgcgggcac ataaacgtcc agcgcaattt ccgcaccggc ggaggtagga
     1381 atggacagcc gttcgcagcg catcataaac aaaatctcct ttggtttgtt tcggaaaggt
     1441 tctgcatcaa tatacccgcc tgagggcgga aagtcaaggt ttttcgcgcg tgagggcgat
     1501 ggaagagaaa atg
//
LOCUS       DAWAEA010000346         1500 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_10271_length_1500_cov_1.933564, whole genome shotgun sequence.
ACCESSION   DAWAEA010000346 DAWAEA010000000
VERSION     DAWAEA010000346.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10271_length_1500_cov_1.933564"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    340..355
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          311 a          395 c          471 g          307 t
ORIGIN      
        1 ctatgtcagg gctttggagc ggcaggagag tatttatcct gcggactacg ccaagctgcg
       61 ttttctggaa aaccatgacc ggccccggat gacccacctg ttccccgacc gccgcacccg
      121 gctgaactgg ctttgctgga tgtttttcca gaagggcacg gctttgctgt acagcggtca
      181 ggagtgggag aacgatcacc ggcccgacct gttcgacccg gatcccattc ccatggaaga
      241 ccccgccccg ttggagggac tgatccggcg gctgaccgcg ctgaagaagg acccgctttt
      301 tgaaattgga gcctattccc ttcgggcctg cccgggcgan nnnnnnnnnn nnnnntggag
      361 ctgccggacg gccggtatga aaacctgctg gagccgggaa cgttcctgcg ggtggaaagc
      421 ggcctgcttt cgctggatgg aaacccggca gtattgcaga gctgaaaacg ccgccggttg
      481 acagggccga aacagggggc tataatctgc ctatacccct gaacggatgg aggcggcaga
      541 tatgaagcaa tgtatggatt cccagaacct gcaccgcagg ctgaaaaaga tcatcgggca
      601 ggtacaggcc atcgaccgca tggtggacga ggacgttccc tgcgaagacg tgctttccct
      661 gatcaacgcc gccaagtccg ccctgaacgg ctgcggcaag gtggtgctgg aagggcatat
      721 caagcactgc gtccgggacg gcatcgagca tggcgacgcg gaaaaaacca tcgagggctt
      781 cactaaggct gtggagcgct tcgccaatat gcaatagacg aaaaaagccg gcagactgtg
      841 tcggcttttt tatttgttca attgacaaca tataccccta tatgtataat aagcatatac
      901 ctaccagggt atagaaatgg gcttcattca tgaaacggct catggagaaa ctggaacact
      961 tgctggaact gggcggcatt cgtaaggacg tgacgctact ggtcatttcc ggggcggcgg
     1021 ttatctgcag cctgttgaaa tttcagcctt ttccctttga tatggcgtgg atcgccattg
     1081 tactatgcgg tctgcccatc attctggaag cggtcgtcgg gctggtgacg gcctttgaca
     1141 tcaaggcgga cgtgctggta tcgctggcgc tgatcgcctc gatctgcatc ggggaggaat
     1201 ttgccgctgg cgaagtggcg ttcatcatgc agttgggcgc tttgctggaa gaactgaccg
     1261 tggcgaaggc ccgggccggg atcgagaagc tggttcatct gaccccgcag acggcgcggg
     1321 tgctgcggga gggccgggag gagatcgtcc ccgccgagca ggtgcgggta ggcgaccgca
     1381 ttcgtgtgct gcccggtgaa agcattcccg tggacggcgt gatcgtttcc gatcagactt
     1441 ccatcaatca ggccgtgatg accggcgaat ccctgccggt ggataagact gtgggggact
//
LOCUS       DAWAEA010000347         1500 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            NODE_10275_length_1500_cov_1.035294, whole genome shotgun sequence.
ACCESSION   DAWAEA010000347 DAWAEA010000000
VERSION     DAWAEA010000347.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10275_length_1500_cov_1.035294"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    298..412
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          321 a          404 c          385 g          275 t
ORIGIN      
        1 cgtttttgtc ggtgagctgc atctcgttca aggcccgctc cacccggtag tactgcaaat
       61 actgcatggg cgtcatgtgg gtcgcctgtt tgaaaaaacg gcagaagtac ttgcggtcca
      121 gatgcaccgc cgaagccaga tcgtccagcg tcaatttctg gctgtagcgg ctttcgatca
      181 ggtcaaagac ggtcttgagc tgcagcattt tcttgtcctc gtcccggggc tgctccaccc
      241 gggtaaaact gccccgggcg taaacgccgc cgaagaagac gtacaaaagc cccagcgnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntcgatctc
      421 ccccattttc tcccggcaca gtccgggggc gctcaggagc atccgcagat cgaaaacgat
      481 gcattcatac acgcagtcgt ggggcgttcc tgcgtgaagg cttcccgctg ggaagtacgc
      541 cgcttcgccg ggatgaagct ccatgctttc cccgtccagc gacagggaaa aggtgccgga
      601 cagtacaaag agaatttccg tttcctcatg ccagtggtag ggcatctcat agcgggcgtg
      661 gtttcggtca atatagtgat agtccagcgg aaattccatc gacccgcgct gcttggcttc
      721 atgatattgc aaagttctca tttccgcaac cctcccgtgc aaaaaagaga atattgtccc
      781 gctatttgcc atgataaaac aggcgggggc aggccgcaag ggctaagatg aacatatcag
      841 gaatctgcaa ataaatgagg tgatttttga tgaattccca ggaaatcaag gatcagatct
      901 gcgacgtatg ccacaaaatg tggcagctgg gctgggtagc cgccaacgac ggcaacgtat
      961 ccgtcaaaat ggaggatggc acctactgga tcacgccatc gggcatcagc aagtccttta
     1021 ttacccccga taagctgctg cgggtggacg cggccatgaa cgtagtggaa ggcccggaaa
     1081 actacaagcc ctccagcgaa atgaaaatgc acatgcgctg ctacgaagtc cgggaggacg
     1141 taggcgcggt ggtgcacgcc catccgccca cggctacggg ctttgccgtg gccaacaagt
     1201 ctctggacga ctactgcatg atcgaaacgg tcatcaccat cggctccatc cccgtcgcgc
     1261 cctacggcac gccctccacc aacgaagtgc cagattcgat cacgcccttc ctgccggagc
     1321 atgacgtgct gcttctgcag aaccacggtg cgctgaccgt gggctgcgac ctgatcaccg
     1381 cctactaccg catggaaacg ctggaacact atgccaagat cagcctgaac gcccacctgt
     1441 tgggcggtgc gcaggagatc ccccgggaga agatcgacca gctgctggat ctgcggaaga
//
LOCUS       DAWAEA010000348         1297 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            k99_2155_length_1297_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWAEA010000348 DAWAEA010000000
VERSION     DAWAEA010000348.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2155_length_1297_cov_4.0000"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          259 a          423 c          359 g          256 t
ORIGIN      
        1 aggcgatacc atagaggatc agcatcaggg cgatcaccag cggcgtatgc aggtgggtct
       61 cgatatagtc gtcgaacagc agggtcacga cggcccccgg aatgcaggcc actactacct
      121 tgagccagat ggagagggtg tccattttga ccgcaggctt tgttttatcc ttgaactgga
      181 aggggaacat cttctgccag aacaggatca ccaccgccag gatcgcgccc agctggatca
      241 ccacgaagaa catatccttg aatgcgtcgc tcacgttcag ggtcagaaat tcgtccacca
      301 acagcatgtg gcccgtgctg ctgatgggca gccattccgt cacgccctcc acgatgccca
      361 gaaaaatgac cttcaaaatc tccgtaaagc ttagagccat ctggaaagac ctccttgcaa
      421 gccggttttc gcttcccctt ccgggaacga tcaccggcag ggcgcttctc aggctatgct
      481 tttcacctga gcattatagc atacccacgc aagatcggcc agcggttccg ccttttcatt
      541 ccagcccaag actttccagc gccgtcatga gagacgcgtc catggcccgg taaggcttcc
      601 acaccagcac aaactccacc ggctcccgga agtacagcag atccttctcc atcgccttgg
      661 ggccgcagct gttggaaccg atgccgccct gccggtaatc cagcgacagc acggtcttac
      721 cctcggattt caggtcatag gtatgcttgg cccgggtcag gagttcgtcg gaataatcgt
      781 gcgcattgaa gctgagccgg ttctcccagc ccttttcaca ggcgatgagc agcccgtctc
      841 ccagcgcgtt ggtcacggtc agggccttca cgccgccgcg ggcgccgttt tcctgcggac
      901 ggatataggg ttcgtgcagc tctgcgacct tcgcttcgta cagacctact tccgcgcccg
      961 ccagcaggtc gggatagttt tcccggggac cccggccata ccacgccaca cggtcaaatt
     1021 cctccggcag ctccagctgc agccccagcc ggggcagcgg tggcagattg tcccgcaggg
     1081 ggatgaactg atttgccagc cgcagcgtgc cgtcggcgga cagggtgtag tccgtttccg
     1141 cctgcagcac cggcccgaag gatgtcggcg cgcagaccca gaccgcgtga acgcagaccg
     1201 cgccgttttc ctgccgccaa ctccattcgg tcagacggcg ctgcaggcgc tccagcccga
     1261 acttgcgcca atggacgctc aaatgtacgt cgttgtc
//
LOCUS       DAWAEA010000349         1160 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            k99_6204_length_1160_cov_3.0000, whole genome shotgun sequence.
ACCESSION   DAWAEA010000349 DAWAEA010000000
VERSION     DAWAEA010000349.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_6204_length_1160_cov_3.0000"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          206 a          341 c          352 g          261 t
ORIGIN      
        1 gtggtctccc agagctgccc cgggccgtat ttgggatagc ggaactcctc gatgagggag
       61 gtctgcacct gctgctggcg gcgctccttg gacatgaaca ccttggagag catgtccctg
      121 aggatgccca tgatgctcag ctccttggtg cgctgcttgc cccagctggc gtcgatctcc
      181 cggggatggc ggccccagag cttctcagta tagccctcaa agaacatgga gtatagcttc
      241 ttgccgaagg aattgatgta gaaattttcc agattgtcgt tgggccgctt gtggatacag
      301 gccgccagat agctgaagcc cacctgcatg gtggtggcaa agcccatggt cttcagcgtt
      361 tcccacttga ggctgatggg atagtcaaaa aagtgatgct gataatagat gcggctgacc
      421 cggttgcggg tgagcatgac ccggtcttcc ttttccgggt cggggccgcc ctcggccaga
      481 ggcacctccc ggttcaagag ccggtcgtcc atggcaggcg caccctgcag gggcatcatg
      541 tccgcccacc actgcatcac ccggtcgtcc ttgctgaaaa agcggtggcc gccgatgtcc
      601 atccgattgt cccggtagcg gacggttttg gaaatgccgc cgatctcctg actgcgttcc
      661 agcacggtca cgtcgtattc gccggttttc agaagctcgt aggcggctgt cagccctgcg
      721 gggcccgcgc cgatgatgag cacttttttc atgtgcgtat tcctcgtttc gtgattcgtt
      781 ggcagaaaat gaaaaaacag gatcgcttct tttgaaacgg atgataaggc ggggaaaggc
      841 ttaccaggcc agcccttcgt ggcccgtttc cgtcaccagc acctgaattt cccactgggc
      901 cgacggcttg ccgtcctggg tgtagatggt ccagtcgttg tccgcgtcga tgtagatgcg
      961 gttggtgccc atgttgacca tgggctcgat ggtgaagatc atgcccgggg ccatgatcat
     1021 gcccgtgccc cggcgagtga catagctgac gaagggatcc tcgtggaact ccacgcccac
     1081 gccgtggccg cccacttccc ggacgacggt atagccgttc ttccgggcca ggctgtgcac
     1141 cgcttcgccc acgttgccga
//
LOCUS       DAWAEA010000350         1146 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            k99_7950_length_1146_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWAEA010000350 DAWAEA010000000
VERSION     DAWAEA010000350.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_7950_length_1146_cov_4.0000"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          262 a          327 c          355 g          202 t
ORIGIN      
        1 gatcatgacc cggtgggcag caacggacag ggtgtgcggc aggtgtatga ggagatcaag
       61 gccgcaggtg ttcaggacgt gacgctgaag ctctacgagg gcggacggca cgagatgttc
      121 aacgagatca accgggaaga ggtctacgcc gacctgatcg gctggctgaa cagcaagctg
      181 gcctgacccc aaagaacacc gaagaaagaa ggcggaaggt tggaatccga gcagttaacg
      241 gcggtggtgc aggacaccac cttccgcaac gatgaaaacg gctatacggt gctgcgggtc
      301 acggtggggc gcaccaccca gacggtggtg gggcaaatgc cggaactaag ccccggcgaa
      361 aacgttacct tcgagggcag ttggacggag catcccgcct acggcaagca gtttcacgct
      421 ctcaaatgca ccatcacccc gcctacgggc aagaccgcca ttgaaaaata ccttggttcc
      481 ggcctgatcc ggggcatcgg cccgtctacg gccaagctga tcgtgcggca tttcggggaa
      541 aacgcgctgg aaattctgga cgagcatccc gaacgcctga ccgaggtcag cggcatcggc
      601 aagaaaaaag ccgccatgat tctggaaagc tacgcccagc agatgggaat gcgccgggtg
      661 ctggtgtttt tacagaacta cggcatcagc cccaatctgg ccatgcgcat cgccaaatac
      721 tatggtgaaa atacggtgga gctggttcgg gaaaaccctt accgcatggt gatggacatc
      781 gacggcgtag gctttctgac cgccgaccgc atcgccctga gcatgggcat ggatccccag
      841 agcgagttcc gcattcgggc cgcgctgttt tatctgctca gcgaggcggc caacggtgcg
      901 ggccatacct acctgccccg agaactgctg ttggagcggg ctgcggccat gctgcacgcc
      961 cccgcggagc tggtggacgc gcaaatttcc cacgcgctgc tggaaaaaat gatgatcggc
     1021 ttttccattc ccgattgcga aaacgctctt tgtctgcctt tatattatct ggcggaaagc
     1081 gagatcgctt tgcgactgac ccgactgatg cacgcccgcc cctacaagcg ggtgcagggt
     1141 ctgaac
//
LOCUS       DAWAEA010000351         1133 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            k99_7819_length_1133_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWAEA010000351 DAWAEA010000000
VERSION     DAWAEA010000351.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_7819_length_1133_cov_4.0000"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          203 a          345 c          330 g          255 t
ORIGIN      
        1 cttctggcca ggacggaagc gttcacaaag gataccgcaa cgccataatc aacggcttcc
       61 gggcaggtta tatccagaaa accatacagc ttcaggggct cggcgctgag ggccatatag
      121 gcctcgtcct gattgagcag gagggtgaag attcccttcc gctcgttttc gtcgctgcgc
      181 atcagcgcta caaggtcgta gaaacggccg ctgagaagga tcctgctgaa ggcttctgcc
      241 ccgtcgcaga tcgcgtcctg ctcgtccttg gggacgccgg tccagccggg atcccacgcg
      301 gtcagcctgg gaaagcgcca cgtctgattc cagtactgcc ggataacgcc agcggcatcg
      361 aagcgaagca tcagcgcgcc gtgccggtcg ttggcgggaa cgtcgacgtg ataatcctcg
      421 ccgatgcgtt tgacctgcat ttccccgtct gtaaccgctt catccacgaa aggattggcg
      481 aagaagcaga gggcgaattg acgcgcggtt gtttcgtcgt cgatgagagt gatggttttt
      541 cccagcgccg ccacgggcag gaaaaggagc agagtcagac agcacagaat ctttcgcatg
      601 accgaacctc cttggatggt atctgcctat tcaacgtttg cacgggcata tttcatccaa
      661 aaacaggaaa aagcagcccc ggcgaaaatt tgccggggct gcacctatct atgaaagttc
      721 ccttacggat tggtcttttg ggcgtcggcc aacttcttgg tcagctcgtc gatctgcgcc
      781 tgcagcacgg cggtctcatc ctcggcggcc ttagcggcgg cagcggcgtc ttccttggcc
      841 ttggccagtg catcgtcacc tgcggcctta gcggcttcca gatcggcctt ggcctgcgta
      901 gcggcggctt ccgcgtccgc cagcgccttt tccagagagg ccttcacgtc ctccagatcc
      961 ttggtcagct gatccttgtc ggcggcagcg gtctcgtttt ccttggtcag cgtctccacc
     1021 tgcgcttccg cttcggtcag cttggcctgc agttcttcca gagaagcggc cttttcgtcc
     1081 acgtcggcct gcagggtctc ggtctgctcg gtcagttcct ttacctgcgc gga
//
LOCUS       DAWAEA010000352         1122 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            k99_667_length_1122_cov_6.0000, whole genome shotgun sequence.
ACCESSION   DAWAEA010000352 DAWAEA010000000
VERSION     DAWAEA010000352.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_667_length_1122_cov_6.0000"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          202 a          388 c          333 g          199 t
ORIGIN      
        1 cgacgggtct gatgtcatat cgcgaggatc gctccccgcc agcaggttca tgaccaatgc
       61 cgcgtccccg caggtcttgc acagcgggcc gatctggtca aaagagctgg ccattgccac
      121 cagaccgtag cggctgacgg tcccatagga gggcttgatc ccggccacgc cgcacagcgc
      181 cgccggctga cggatgctgc cgcccgtgtc gctgcccagc gccaccggcg tcatgcccgc
      241 agccacggcg gcggcgcatc cgcctgatga cccgcccggc acccggcttg catcccacgg
      301 attgcgggac gggccgaaaa agctggtttc ggtgctgctg cccatggcga actcatccag
      361 attcacccgg cccagcagcg gcattcccga ctcctgcagc cgccgcaccg ccgtggcggt
      421 atagggcgag cggtagtttt ccagcatccg actggcgcag gtgcaaagtc tgccctccac
      481 gcacagatta tccttcacgg caaaaggaac gccgtccagc cgggacaggg gctttccctc
      541 ccggcggcgg cggtcggacg cccgggcttc ctcccgggcc gtatccccga aaacctccag
      601 aaacacgttc agtgtggagt tttgcagggc gatattttcc aaataagcgt ctgtaacggc
      661 ttcagacgaa aaactcccgt tttccagccc acgcgccagt tccgaaacag tcagcgcgca
      721 cagggcgagg gagcggcggt tccctaccat gagcaaacag tctcctttga tcttatgcca
      781 gcgtgtgcgg cacggtaatc agcccgtctt cggcggcagg ggcgttagcc agcgcctgat
      841 cccggtcaag gctggcggac ggctcgtcgg cccgaagcgc ggcacaacct gtctcccggc
      901 ggaactccgt ctcggcggtc tttcccgtaa ggggcggcag cgccgccgag cgcagcaccg
      961 agggccattg ccgccggagg atttcttcct cctccggggt cattgccagc attcccgcat
     1021 cgtccagcag acgactccat tccgatttca tagatagtgc tcctgttctg gttccatcct
     1081 gattttgaca acagcattga agcccgcaac gcgctgaaag ca
//
LOCUS       DAWAEA010000353         1122 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            k99_18473_length_1122_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWAEA010000353 DAWAEA010000000
VERSION     DAWAEA010000353.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_18473_length_1122_cov_4.0000"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          161 a          367 c          326 g          268 t
ORIGIN      
        1 gccgtccgct gcgcgggact gctgctgggg ctgatggcgc tgctgcttct gccggactat
       61 cacgagtcga tctactggtt cggcggggct atcaactaca ccgtcatgtc ggcgctggcc
      121 atgctcctgt tttccgccgt gttccgggcg gcgctggata ggaaagcgcc ggtctggcgg
      181 ctctgcctgt ggtgcgtcgg atttttctgc cttggcggca ccaactggat gacccccgcg
      241 tcctccatcg tgctctatgg ggcaatggcc gtctggattc tgattacccg ccgcccgaag
      301 cgcctgttgc tgccctacct gtttctgctg ctgggctttg ccacagcggc gctggctccc
      361 ggcaacgccg cccgtcagga aacgatgaac gccaacgtca gctttatcag cgcgtttctg
      421 tcctccctcc agcacagcgt tctctatttt ttctccgacg ctcggtactg gctgtttttg
      481 ctctttctgc tgccggtctt tcgggaaatc tacacccgca cgcgcttttc cttccgcgcg
      541 ccgctgctgg tctttgccgc gtccgtctgc gtcatggcag cggccatgtt cccggtgctg
      601 taccagtgca gctattgggc tccacggcac acagacctgt gttttctcct gctgtgcgtt
      661 cttctgccgg tcaacgcttt ctggctgctg ggctggtttc tgcgccgggc gggctatgag
      721 ccgaaagccc cggactcctc cctcaaaaac agccgcgccg tctttggcat gggcgccgcg
      781 ctgcttttcc tgatcggggc gctttccctg ccgaacatga ccttttcccc gctgcgattt
      841 gactgcaccc tgcagccggt caaagtcgcg gcccgtctgc tggacggcag catgagccgg
      901 tacagtcagg cctacgacgc gctggtcgcg gagatcaagg cccatcccgg ggaagaaatc
      961 cacagcgcca gccagcccca ggacgatatt ctcaacgcca actggctgta caccggcgat
     1021 ccgcaggatt ggcggaatca ggggctttgt ctgtactacg gcgacggctg cacgctcgta
     1081 tacgacccgt aattttcaga aaagaggatg ctttcgtgga aa
//
LOCUS       DAWAEA010000354         1113 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            k99_4642_length_1113_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWAEA010000354 DAWAEA010000000
VERSION     DAWAEA010000354.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_4642_length_1113_cov_4.0000"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          193 a          364 c          325 g          231 t
ORIGIN      
        1 ccctggttga gcagcaggta ttccagaatc ccgttttcct tccgggtcag ggccacggcc
       61 tcgccgttga cctgtgccgt ccgcttcacc gtgtcgaagg tgacccgccc gcagctcagc
      121 agcgcgtcgt gctgcacgaa gctgcgtcgg gtcaggctgc gcacccgggc ctccagctcc
      181 tccagatgaa agggcttgac cagatagtcg ttggcgccgc aatccagccc ctctacctta
      241 tcgctgatcc ggctgcgggc ggagagaatc agcacccggg tctcccgatc ccgctcccgc
      301 agcgccttca acagctccat gccgtcccgc ccgggcaggt tcagatccag caggatcagg
      361 tcgtacttct ccgccagcag ccagtccagc gcggaatcgc cgtcatagca gacgtctgcc
      421 tcgtagccct tctgcttcaa actcttggcc agcgtgtcgc acagcgcccg ctcgtcctcc
      481 acaatcaaaa tccgcatgcc gcgcctcctc tgcgttcgtg tgattacgtc cagtatagca
      541 taccggtata aaatgcgcat aaagttttcg gttttaacgt ggattttaac ccccatatcg
      601 tataatgatc ctgcgagaaa aaacaaagga gggattcccc atgaatctca tttcatcggc
      661 cgcgggcagg cgcgccgcga ggctgggcgt gctgatactg ggcgcggcga tgctggccta
      721 cggcgcgtgg cggggcgagg ccgccacggt gctgtccaag gccatccgca tctgtctgga
      781 gtgcgtgggc attggatagg aagaaggcaa agcccaaggc accgcccgtt tccggctttc
      841 tcgcccggtt ccgggggctt ctgcaggcct gcgccgtact gctgaccaat ccgcacctgc
      901 ccaacctgct caaggggcag atttaccgag ggaaagccaa gaacgtctgc gtgccggggc
      961 ttaactgcta ctcctgtccc gccgccacgg gggcgtgtcc cattggggcg attcagtcgg
     1021 tgatcggatc gtccaaattc aagttttcct actacgtcac cggcacgctg atcctgctgg
     1081 gggtgctgct tgggcggttc gtgtgcggtt tcc
//
LOCUS       DAWAEA010000355         1078 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            k99_657_length_1078_cov_3.0000, whole genome shotgun sequence.
ACCESSION   DAWAEA010000355 DAWAEA010000000
VERSION     DAWAEA010000355.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_657_length_1078_cov_3.0000"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          248 a          299 c          345 g          186 t
ORIGIN      
        1 agcgcggcga tcaccgccag aggcgatgcg gacgtccgcg tggctgcgga tggtctgagc
       61 gagattttca ccacgcttcc ggacggcact gcgctgaagc tgctggccgt cgagggcgac
      121 tgggcgaagg tggaagtgga cggtcagatc ggctacatct acaaggacag cgtgtccggc
      181 gtggaatggc cgaaggccga gccgaccgag aaccccgacg aggagaacat gaaggtcacc
      241 atcttcacct cccgccgcac ggtggtcgag cccggcgaga ccatcaccct gaccagccgg
      301 ctggagggct tcgaaaactg tgaaatcgtc gtttaccagt ggcagtatga tcgcggaaac
      361 ggctttgaag atatcccggg cgcggacgcg gacacttacg cctttgcggc ggatgtggag
      421 acgctgagct acgactggag actggtagtt tactacgcat aatccgttcg ccaaccgatc
      481 gcaaacgaat tgctgaaaaa gctaggagga atcgcaatgg caaaaatgaa aagattcatg
      541 gcaatgctga tggcaatgct gatgattctg caagtgatgc ccgtcgaagc gatggcggag
      601 ggaattgaag gcctgaagag tcgggtctcc aacgtcatca ctggcagcac cttcttcacc
      661 ctgacgttca ccgtcgaggg tgaggacggc aatgacgacg tcatcacgca gtacgtcgag
      721 gccggccata cgccggtgct ccccgaagcc cccgcgaagg caggatatac ctttgtgggc
      781 tagtttactg ccgatgatac cgaggtgacg aaagaaacgc tgatcacttc ggacatggcc
      841 gcggtcgcgc ggttcacgtc catcgcgacg gtgaccgtca cggtgaacta tgtcgccgag
      901 gacggaacgc aggtggccga ccccacggtt cgcgagtatc tggcgaatag cgaggacatc
      961 atcaactgcc ccgcggaagt ggtacataaa ggcgtgacgc tctatcccga gcagacctcc
     1021 gtcacggtga acgccgacct gctgaataag gcagtggacg gcgcggtgac cgtcacgg
//
LOCUS       DAWAEA010000356         1076 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            k99_5528_length_1076_cov_3.0000, whole genome shotgun sequence.
ACCESSION   DAWAEA010000356 DAWAEA010000000
VERSION     DAWAEA010000356.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_5528_length_1076_cov_3.0000"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          196 a          304 c          338 g          238 t
ORIGIN      
        1 acgccgattt gtatgttgta tgccggcagc agctggtcgt tgcccatgta atcctttttc
       61 aggcgcatga acgtggcgga aggatccgtc ctggcatagc tgtttcgctc gtcgccgcag
      121 atcgtcagct tctagcggta ttcctccagt gtgcgcgtat atgctttaag gcgctcgtaa
      181 cggcgctgcc ggatcgtttt gcggcgtcct ctgccgggta caaaagccgc ttcgtccagc
      241 aaagtcgtga tttttgcaaa cagccggtaa cgggatttct ccgtcgcctt cttccacacc
      301 caggaatact tgttggcgtt ggccttcagc ttcgtcccat cgatgtagag gtgcgataaa
      361 tccaccttct cctgcgcaaa gatggccgga ttcaaaaaaa tcccagacct ttcagcctga
      421 ggaattgttc agacagtccg aaaacgatca tagatatccg agaaatcatc gggaatattg
      481 ggggcggaag agggttcgga gttttcggac atatgagagg cgtttgaagc ggaggcattg
      541 acgcggggcg gattctgcgc ggcgggccct tttttcggtt gggcgggctc ttccccgtcc
      601 gcggcttctt tcatcgccgg cgcgtaggga atcaggccca ttccggccgc ttcccgctgg
      661 atttccgggg gaagatccct ggcgtccacg atgaagccgt tccaggagag catccagagg
      721 gcgggactgt cctcaatcag ctccggcagc agccattgcg cgtccagcgg agacagccga
      781 accagtctgc ggatttcggc ggccctggcc gacgcggtgg cgggcgcgag gccgaagggg
      841 cggctcagct cgtcggcggt gcggtgaatg ggcatggatc tgtcgaatat gaggttgttg
      901 gccgcaatgg cgtagacgat tcccgccgcc caggtgttcc tccggccctt cagcagcggc
      961 gaggggcgct tgcggcacag cttttccagc agccgcaggc acagcgcgcg gtagtcctcg
     1021 ttcaggaacc ggccgcagta atccgaaatc atttcggcaa gctcgccgta gatcgg
//
LOCUS       DAWAEA010000357         1065 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            k99_10347_length_1065_cov_3.0000, whole genome shotgun sequence.
ACCESSION   DAWAEA010000357 DAWAEA010000000
VERSION     DAWAEA010000357.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_10347_length_1065_cov_3.0000"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          232 a          323 c          312 g          198 t
ORIGIN      
        1 attccgagct gggccgtcag gatgaaagct actatgtctg cgttaccgca caggatgcga
       61 agacctatct gggcttcaag cacgaggact cctgcgaaaa gttcttcgcc gccgccgaaa
      121 aggcgcagga gacccatgag ctgatcgact atacccagta catcaacgcc gttccctccg
      181 tgcccggcac gcaggtgatg atccccgccg gtacggtgca tgcctccggc cgcaatcagg
      241 tcattctgga gatcggcagc ctgaccgtgg gttcctacac ctacaagctg tacgactatc
      301 agcgcatcga tccccagacc ggcctgcccc gccccatcca cctgaagatg ggccgcgacg
      361 tcatccaccc cgagcgcccc gccgaattcg taaaggacaa tctggtcaac cacggcggta
      421 tcgtccgtca gggcgaagac tggtgcgaga aggtagtcgg cgagcacgac cttctgtatt
      481 tctccctgcg caatctggtg ttcaccacct ccatggagga cgacaccaaa gacgatttcc
      541 acgtgctggc gctggtggac ggcgaaaagg tgcgtgtgga atccgtggag gatcccagcc
      601 gttactttga cctgaacatg ctggacatcg ttgtggtgcc cgccaccttc ggcaagtacc
      661 gcgtggtgaa caagggcatc ggcgtggcgg tggttcacaa gaccatgctc aagccgtaag
      721 gcggaggaga tcatccatgt acgagcttca gggaaaaacc atgatctacc ggctggacag
      781 ccggggccgt tccgtcagct gctatcaccg ccagaccggg catgagtata tccacacccc
      841 cggcgcggtg tggaagctga tctacgctgt gaagaacacg gaacgggtag aggttcccgt
      901 ctgggcggag gagcagcgct tctccgtcca gaccggggaa aacagcctga ccctttgcta
      961 tgacgggctt accggcgatg agggccggaa gatcaacgca tctctgaacc ttcattttca
     1021 gatgaacgat ctgggcctgc aggtctgggc cgatctcgaa aaccg
//
LOCUS       DAWAEA010000358         1064 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            k99_4876_length_1064_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWAEA010000358 DAWAEA010000000
VERSION     DAWAEA010000358.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_4876_length_1064_cov_4.0000"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          216 a          328 c          332 g          188 t
ORIGIN      
        1 ggcccgggag atcgccgcgg cgggcgtgga gatcatctcc ctgaccaagt tcggttcctc
       61 caccatcagc gacctggcct ccatccggct gttcaccggc agcaccgaga ccttcatgcg
      121 cagcggcgcc atgagctccc gtatctgcca gatgttcatg atcgacgtgc tgtatacgtc
      181 cgtgtgcagc cgcacttata aagagattcg tccccatctg gaaaagaccc gggaggccgc
      241 cctgcggatg catacggccc gcggcgagac caacaaggac taaaaaacgt tcatctatat
      301 atactaggag ggtatttcta tgaacaaggt tctcaacggc atcgaccggc cggaagcact
      361 ggatcaggaa ctgaagggcc tgcggctggg cgtggtcacg ggcggcggcg cgatcaaccg
      421 ggaactgatc cccgtggtgg acgtgctctg ccagcgctac aacgtcacgg ccctgtggaa
      481 caccatctac ggcatccggg gcgaatttca gtacggcgaa gacgtgccgg aatacaccga
      541 taccgtcacc gggcggcagg tggtcagcat cttcaaccgg gagcgcatcg ccccttcgga
      601 agaaatgctc gcccaggtgg acgcggtggt cttcgacatt cgggaagcgg gcacccgctt
      661 cttcgaatat ctgcactgct gcgccgcgct gatgaaagcc tgtgctaagg cgggcaagaa
      721 gctggtcatc cccgaccgta tcgcgcccat caacggcgta acggtggagg gcacggtctg
      781 cccgtccacc atgcacctga tcgtaggcga ctatgagctg cccagccgaa cggcgctgac
      841 catgggcgaa tttgcccggt acgtgaacgg cgagttcggc gtgggctgcg atttgcacat
      901 catccccgtg gagggctgga aacgcggctg gtacgcggat gagaccgacc tgccgtggct
      961 gctgccctcc cccagtctga acagctgcac cgccaacctg ttgtatgcag ggatgtgcgt
     1021 attcgagggg gtcagcacca tcagcgaagg ccggggcacc agca
//
LOCUS       DAWAEA010000359         1032 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            k99_17703_length_1032_cov_3.0000, whole genome shotgun sequence.
ACCESSION   DAWAEA010000359 DAWAEA010000000
VERSION     DAWAEA010000359.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_17703_length_1032_cov_3.0000"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          222 a          285 c          266 g          259 t
ORIGIN      
        1 cttcgtatac tcctccggtg ttcgttcttt tttgcgctga gaacagcagc aacacttttt
       61 aggttccatg cacagcacct ctttcggttt gcatacccca tctgggtatt ttgattatat
      121 acccccacag ggtatatgtc aatttatttt ttcattttct ctcaaaaaag ctgcctgtcc
      181 tgcaaaccag gtagagaagg tcgaaaatga tccgaaagtg tcttcaaaag gcataaatgg
      241 acactttcca aaataaaacg cccgtgctat aatgacttca catcacacca aaggagcgat
      301 tttcatgggc gtttttgatt ttcccctttc gaagctgaaa acatacgaag gcatcaatcc
      361 caagcctgtt gattttgaag aatactggga gcgtggtctg cgcgagatcg aaaccatcga
      421 cccgcaggtg cagctgactc ccgctgcctt ctgccacccc acgttggact gctttgacct
      481 gaccttcacc ggtgcgcacg gcgcgcgaat ccacgctaaa tatgtgcgac ccaagaaatt
      541 ttcgggccgt ttgcccgcag tcatgcgttt tcatggttat tcgggccgtg cgggagactg
      601 gaccaatcat tttgcgatgg cctgtgcagg ctttgccgtg gcggcgctgg atgtgcgcgg
      661 tcagggtgga cagagcgtag acgttggcgg tgtcaagggg aacacgctgc acggccatat
      721 catccgcggt ctggacgatc ctgatcccga ccggctgctg taccgggata tttttctgga
      781 caccgcgttg atgacccgca tcatttcgtc cttcccggag atcgacgaag gccgtctggc
      841 cgcggacggc ggaagtcagg gcggcggtct gacgctggcc tgtgcttctc tgagcaacat
      901 tcagctggcc gctccctgct atccgtttct gagcgactat cagcgcgtct gggaaatgga
      961 tctggacaag gatgcctacg ctgaactgaa agagtacttc cgccacttcg accccaccca
     1021 tgcccatgag ca
//
LOCUS       DAWAEA010000360         1026 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            k99_14748_length_1026_cov_3.0000, whole genome shotgun sequence.
ACCESSION   DAWAEA010000360 DAWAEA010000000
VERSION     DAWAEA010000360.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_14748_length_1026_cov_3.0000"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          240 a          286 c          311 g          189 t
ORIGIN      
        1 atgtataaaa caataaaaat aatacgataa gtaaatttga aagcctagcg aatgggaccg
       61 gcttcgcggc cagagcacgg aaaatctgtc cggggagcat tcaaaggcct tttttggcct
      121 tccagtggtt ccacgccttt tcgatttcca cgataaacag ggggctcagg gccaggcccg
      181 ccaccaccag ccactgaacc atgttcagcg gaaccgtctt aaagaccgca gacagagcgg
      241 gaatggaaat gacggagacc tggagggcca ggcagaccag gaaggaatac agcattttca
      301 ggttgccgaa aataccgatc tcaaagagag atttctcact gcggacgtta aaggcatgga
      361 tcagctggga gcagctcagc acggcgaagg ccatggtccg gcccgccgct ggggctccgg
      421 cgccatcgaa gaacacccgg ccgatggtga aagccagcag agagatcatg ccgatgaagc
      481 agccctcgat ggcgatgctg taggtcatac ctccggcgaa caggcttttt ttggcgtcgg
      541 tcggcggctt tttcataata tcctcatcca ccggctccac gcccagggcg agagcgggca
      601 gactgtcggt gaccaggttc acccaaagca gctggattgc cagcagaggg gtgggcagcc
      661 gcatgaggaa agcggccagc acggtgatga tttctccgat attgcaggac agaagaaaat
      721 ggacggtttt tttgatgttt tcaaagattc cccgtccctg ccgcaccgcg gccacaatgg
      781 tggaaaagtt gtcgtcggtc agaatcatat cggcggcgcc tttggctaca tcggtgccgg
      841 aaacccccat agcgcagccg atgtcagcgg ccttgagggc gggcgcgtcg ttgatgccat
      901 cgccgatcat cgcggtcggc gacgtctccg agaacccggc gacgatacga gccttgtcct
      961 cgggacgcag ctcggcatgc acgtcgccga tcccggcctg cttcgccagc gcagcagcgg
     1021 tgcggg
//
LOCUS       DAWAEA010000361         1007 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            k99_17898_length_1007_cov_3.0000, whole genome shotgun sequence.
ACCESSION   DAWAEA010000361 DAWAEA010000000
VERSION     DAWAEA010000361.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_17898_length_1007_cov_3.0000"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          202 a          274 c          264 g          267 t
ORIGIN      
        1 caaaagctcc atctgcatgc ccgcgtcttc cacgctcatg gggcgcatag ggaaggtctt
       61 gtgccgaacc accttgggct cttcttccag ctcggccaca ggttcttcgg catattcgag
      121 gtcgatcgcc ttgaaggcgt cgctgcggaa acgatcctgc agcttggtgc ggtgcttgtg
      181 gatctggcgc tccagcttgg ccagcgcctg atcgatgcaa acgtaaagat tgtcgtcact
      241 ggaagcctcg gcccgcagcg tcacgccctc aaagggaacc gtgatctcac atttccggcg
      301 gccgtccttt tttttcgtca gacggactaa aagctcggtg ttgggatcca gataacgttc
      361 cattttggcg gtttttttca gaacacgttc gctgatggcg ttggaaatga ccatatcttt
      421 gccggtaatc gtagtcttca taaaatggca gctcctttaa caaatgatag gcgttagggt
      481 cagaaaatgt gcgggtgcca atggtaccac gctcctttcc ggttcgggtg agtgaaccgc
      541 ctgatatttt tctctcgctt cctcatctgt ttctcttgcc gattatacat ttcgacacga
      601 gggaaaaaat ccctgctttt cccttgcaaa aagtgttttt tttctttata attgggcagt
      661 acggcctttt aagccgcagt acgcttcagg aaaggagttc ctttatgcgt cataaaccga
      721 ccctcacgca ggaagaaaaa cttgttcgaa tgacggaaag ccccctgccc gggctgattt
      781 tttcgctggc ggtgcccacc atcgtcagta tgctcatctc cgccctgtat aatatggcgg
      841 acacctattt tgtggggctg atcggcaacg ccagcgccac gggcgctatc ggcgtggtgt
      901 ttcccctgat gagcctgatg caagccattg ccttcatgtt cggccacggt tccggcaatc
      961 atatgtcccg ctcgctgggc gcgggcgacg tggacgacgc ggaaaaa
//
LOCUS       DAWAEA010000362         1002 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            k99_20707_length_1002_cov_2.0000, whole genome shotgun sequence.
ACCESSION   DAWAEA010000362 DAWAEA010000000
VERSION     DAWAEA010000362.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_20707_length_1002_cov_2.0000"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          218 a          305 c          291 g          188 t
ORIGIN      
        1 ctgcggagca aagcgccgct ggtgcccgct tacatgagcg ccaagccccg tcttttccgc
       61 agaatccacc tttactatgg cgcgcccatc caaaccgccg cgttggctga aaagggaatc
      121 aataaggaaa cctgcgacga gcttttggaa aatatccggc agtgctaccg ggatatggcc
      181 cagaaatacg gcgctgattt tcccggcaaa atgaaagatt gatttttttc tgcatttttt
      241 cgaatttcct ttcagtcaaa aagaaggaaa atgaaaaaac gcggcgaatt gatacacgtg
      301 tatatctgga ggcgaacgga tgggcattga aatcgcccga catgcgggct tctgcatggg
      361 ggttcggcgc gcggtagacc gcacgctgga agcggctgaa agcggaggcg agatcgttac
      421 cttcggcgag gtcgtccata atcctcaggt gatcgaaaag ctggaagcca tgggcgtata
      481 cgccattcat tcctgtgaac aggccgccgg gaaaaccgtc atcatccgaa gccatggtgt
      541 gtcccccgac atcacccggc agtcggagca gaccgcccgg caggtgatcg acctgacctg
      601 tccctttgtg gcgcggctcc acgacgtggt ttcccgctac agcgcggacg gctccccggt
      661 gatcctcatc gggcagagcg accaccctga agtggtgggc accatcggct gcgtctgcgg
      721 cccctgctac accatctgca ccccggagga agcccaacag cttccgcccc tgaaacgggc
      781 gctggccgtc tcccagacca ctttccccca cgagcgttgg gaggctatcc tgccggttct
      841 ccgcgaaaga gtggaagacc tgaccgtgca ggacaccatc tgtaccgcta ccgtgctccg
      901 gcaggccgaa gcgcgccgtc tggcggcctc ggtggacgcg atgctggtgg tgggcggcaa
      961 aaccagcgcc aacacccgca agctttacga gacctgcaag tc
//
LOCUS       DAWAEA010000363         1001 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Candidatus Limiplasma sp. isolate gSpreadC_ID_635
            k99_13069_length_1001_cov_2.0000, whole genome shotgun sequence.
ACCESSION   DAWAEA010000363 DAWAEA010000000
VERSION     DAWAEA010000363.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993128
            Sequence Read Archive: SRR3737004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limiplasma sp. (human gut metagenome)
  ORGANISM  Candidatus Limiplasma sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Candidatus Limiplasma.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Candidatus Limiplasma sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_13069_length_1001_cov_2.0000"
                     /isolate="gSpreadC_ID_635"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2952930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          210 a          293 c          303 g          195 t
ORIGIN      
        1 attatggacg cgcagcaggc ggacggctgg ctctgcccct gcacccaaga ggaacgcgca
       61 gcctatgacg tgtgggcggt gttcatcatt tgcaaagcac tgatggtcta cggtcaatgc
      121 agcggagata gcagggcggc agagaccgtt tatcgggcca tgaaaaatct ggagggccat
      181 ctgacccact gcaccctgtt caactggggc caaagccgct ggttcgaagc cctgatcccc
      241 ctgcaatggc tgtacgagca gcggcccgaa ccgtggatga tcgagctggc cgtaacgatc
      301 gcgtcgcagg gtctgtccta cgagcgagtg ttcgcctgct ggaaggatca gcagccagac
      361 ccccggggcg tgtggcggca gcagactcac atcgtcaacc tgatgatggc gctgaaaagc
      421 gaagccgtat tctccggcct ttccgggcag gaacccggtg cgttcgcccg aaaaatgtac
      481 gagcaactgc gaaagtacca cggaacgccc atgggacaca ttcagggcga cgaatgtctg
      541 gcggggaaaa gccccatccg cggcaccgag ctttgcgctg tggtggagga aatgtattcc
      601 tgcgagctgc tggcctccat caccggcgag cccttttggc tcgatctgct ggaacggctg
      661 gcgttcaact ctctgcccgc cacgatctcg gcggacatgt ggtcacacca gtacgatcag
      721 cagctgaatc agatctcctg ctccattcag cagggaacgc ccatatttcg caccaacggc
      781 agtcagtcca atctctttgg gttggaaccg aattatggct gctgtaccgc gaatttttcg
      841 cagggctggc ccaagctggc actgtccgcc ttcatggaaa cagaaaaggg actcctgtcg
      901 gcagtgctgg ttccttcctc tgttcagctg gaacgcggcg gcgaaaaggc gcgggtcacg
      961 ctggaaaccg agtatccctt ccgggacagt ctgctgtata g
//
